Gfra3 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Gfra3
Entrez Gene ID	14587
Full Name	glial cell line derived neurotrophic factor family receptor alpha 3
Synonyms	GFRalpha3,Y15110
General protein information	Preferred Names glial cell line derived neurotrophic factor family receptor alpha 3 Names GDNF family receptor alpha-3 GDNFR-alpha-3 GFR alpha-3
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 18 Map Location: 18\|18 B1
Summary	This gene encodes a cell surface glycoprotein and member of the glial cell line-derived neurotrophic receptor (GDNFR) family of proteins. The encoded preproprotein is proteolytically processed to generate the mature protein. This protein mediates binding of the ligand artemin to the ret receptor tyrosine kinase, and this interaction may regulate thermal pain and axon regeneration. Homozygous knockout mice for this gene exhibit impaired proliferation of cultured neuroblasts and impaired development of the superior cervical ganglion. [provided by RefSeq, Aug 2015].

mRNA and Protein(s)

mRNA	Protein	Name
NM_010280.4	NP_034410.3	GDNF family receptor alpha-3 preproprotein

Pathways from BioSystems

Homologies

Homo sapiens (human)	GFRA3	NP_001487.2
Canis lupus familiaris (dog)	GFRA3	XP_005626660.1
Pan troglodytes (chimpanzee)	GFRA3	XP_517954.2
	GFRA3	XP_001112630.1
Mus musculus (house mouse)	Gfra3	NP_034410.3
Rattus norvegicus (Norway rat)	Gfra3	NP_445850.1
Bos taurus (cattle)	GFRA3	NP_001077216.1

Gene Ontology
Bibliography

Related articles in PubMed

Visceral motor neuron diversity delineates a cellular basis for nipple- and pilo-erection muscle control.
Furlan A, La Manno G, L?bke M, H?ring M, Abdo H, Hochgerner H, Kupari J, Usoskin D, Airaksinen MS, Oliver G, Linnarsson S, Ernfors P
Nature neuroscience19(10)1331-40(2016 Oct)

Inflammatory and neuropathic cold allodynia are selectively mediated by the neurotrophic factor receptor GFR?3.
Lippoldt EK, Ongun S, Kusaka GK, McKemy DD
Proceedings of the National Academy of Sciences of the United States of America113(16)4506-11(2016 Apr)

Hox Proteins Coordinate Motor Neuron Differentiation and Connectivity Programs through Ret/Gfr? Genes.
Catela C, Shin MM, Lee DH, Liu JP, Dasen JS
Cell reports14(8)1901-15(2016 Mar)

Expression and functional studies of the GDNF family receptor alpha 3 in the pancreas.
Nivlet L, Herrmann J, Martin DE, Meunier A, Orvain C, Gradwohl G
Journal of molecular endocrinology56(2)77-90(2016 Feb)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

cold allodynia is mediated exclusively by a single molecular pathway, suggesting that artemin-GFRalpha3 signaling can be targeted to selectively treat cold pain.
Title: Inflammatory and neuropathic cold allodynia are selectively mediated by the neurotrophic factor receptor GFR?3.

GFRa3 is expressed at the surface of a subset of Ngn3-positive endocrine progenitors as well as of embryonic alpha- and beta-cells in the mouse pancreas.
Title: Expression and functional studies of the GDNF family receptor alpha 3 in the pancreas.

We found that artemin, the specific GFRalpha3 ligand that evokes heat hyperalgesia, robustly sensitized cold responses in a TRPM8-dependent manner in mice.
Title: Artemin, a glial cell line-derived neurotrophic factor family member, induces TRPM8-dependent cold pain.

Suggest that enhanced GFRalpha3 signaling in visceral afferents may contribute to development of colorectal hypersensitivity.
Title: Modulation of visceral hypersensitivity by glial cell line-derived neurotrophic factor family receptor α-3 in colorectal afferents.

GFRalpha3, the receptor for artemin, is increased following axotomy and regeneration in heat-sensitive C-fibers.
Title: Enhanced artemin/GFR?3 levels regulate mechanically insensitive, heat-sensitive C-fiber recruitment after axotomy and regeneration.

The following Gfra3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Gfra3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu05882	NM_010280.4 Latest version!	Mus musculus glial cell line derived neurotrophic factor family receptor alpha 3 (Gfra3), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Gfra3 ( NM_010280.4 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_034410.3 Mus musculus glial cell line derived neurotrophic factor family receptor alpha 3 (Gfra3), mRNA.

Price & Availability↑

CloneID	OMu05882
Clone ID Related Accession (Same CDS sequence)	NM_010280.4
Accession Version	NM_010280.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1194bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-29
Organism	Mus musculus(house mouse)
Product	GDNF family receptor alpha-3 preproprotein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC145591.5 and AK046542.1. On Aug 15, 2015 this sequence version replaced NM_010280.3. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC066202.1, AK046542.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGGGCTCT CCTGGAGCCC GCGACCTCCA CTGCTGATGA TCCTGCTACT GGTGCTGTCG 
TTGTGGCTGC CACTTGGAGC AGGAAACTCC CTTGCCACAG AGAACAGGTT TGTGAACAGC 
TGTACCCAGG CCAGAAAGAA ATGCGAGGCT AATCCCGCTT GCAAGGCTGC CTACCAGCAC 
CTGGGCTCCT GCACCTCCAG TTTAAGCAGG CCGCTGCCCT TAGAGGAGTC TGCCATGTCT 
GCAGACTGCC TAGAGGCAGC AGAACAACTC AGGAACAGCT CTCTGATAGA CTGCAGGTGC 
CATCGGCGCA TGAAGCACCA AGCTACCTGT CTGGACATTT ATTGGACCGT TCACCCTGCC 
CGAAGCCTTG GTGACTACGA GTTGGATGTC TCACCCTATG AAGACACAGT GACCAGCAAA 
CCCTGGAAAA TGAATCTTAG CAAGTTGAAC ATGCTCAAAC CAGACTCGGA CCTCTGCCTC 
AAATTTGCTA TGCTGTGTAC TCTTCACGAC AAGTGTGACC GCCTGCGCAA GGCCTACGGG 
GAGGCATGCT CAGGGATCCG CTGCCAGCGC CACCTCTGCC TAGCCCAGCT GCGCTCCTTC 
TTTGAGAAGG CAGCAGAGTC CCACGCTCAG GGTCTGCTGC TGTGTCCCTG TGCACCAGAA 
GATGCGGGCT GTGGGGAGCG GCGGCGTAAC ACCATCGCCC CCAGTTGCGC CCTGCCTTCT 
GTAACCCCCA ATTGCCTGGA TCTGCGGAGC TTCTGCCGTG CGGACCCTTT GTGCAGATCA 
CGCCTGATGG ACTTCCAGAC CCACTGTCAT CCTATGGACA TCCTTGGGAC TTGTGCAACT 
GAGCAGTCCA GATGTCTGCG GGCATACCTG GGGCTGATTG GGACTGCCAT GACCCCAAAC 
TTCATCAGCA AGGTCAACAC TACTGTTGCC TTAAGCTGCA CCTGCCGAGG CAGCGGCAAC 
CTACAGGACG AGTGTGAACA GCTGGAAAGG TCCTTCTCCC AGAACCCCTG CCTCGTGGAG 
GCCATTGCAG CTAAGATGCG TTTCCACAGA CAGCTCTTCT CCCAGGACTG GGCAGACTCT 
ACTTTTTCAG TGGTGCAGCA GCAGAACAGC AACCCTGCTC TGAGACTGCA GCCCAGGCTA 
CCCATTCTTT CTTTCTCCAT CCTTCCCTTG ATTCTGCTGC AGACCCTCTG GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_034410.3
CDS	168..1361
Translation	MGLSWSPRPPLLMILLLVLSLWLPLGAGNSLATENRFVNSCTQARKKCEANPACKAAYQHLGSCTSSLSRPLPLEESAMSADCLEAAEQLRNSSLIDCRCHRRMKHQATCLDIYWTVHPARSLGDYELDVSPYEDTVTSKPWKMNLSKLNMLKPDSDLCLKFAMLCTLHDKCDRLRKAYGEACSGIRCQRHLCLAQLRSFFEKAAESHAQGLLLCPCAPEDAGCGERRRNTIAPSCALPSVTPNCLDLRSFCRADPLCRSRLMDFQTHCHPMDILGTCATEQSRCLRAYLGLIGTAMTPNFISKVNTTVALSCTCRGSGNLQDECEQLERSFSQNPCLVEAIAAKMRFHRQLFSQDWADSTFSVVQQQNSNPALRLQPRLPILSFSILPLILLQTLW

Target ORF information:

RefSeq Version	NM_010280.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus glial cell line derived neurotrophic factor family receptor alpha 3 (Gfra3), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_010280.4

ORF Insert Sequence:

ATGGGGCTCT CCTGGAGCCC GCGACCTCCA CTGCTGATGA TCCTGCTACT GGTGCTGTCG 
TTGTGGCTGC CACTTGGAGC AGGAAACTCC CTTGCCACAG AGAACAGGTT TGTGAACAGC 
TGTACCCAGG CCAGAAAGAA ATGCGAGGCT AATCCCGCTT GCAAGGCTGC CTACCAGCAC 
CTGGGCTCCT GCACCTCCAG TTTAAGCAGG CCGCTGCCCT TAGAGGAGTC TGCCATGTCT 
GCAGACTGCC TAGAGGCAGC AGAACAACTC AGGAACAGCT CTCTGATAGA CTGCAGGTGC 
CATCGGCGCA TGAAGCACCA AGCTACCTGT CTGGACATTT ATTGGACCGT TCACCCTGCC 
CGAAGCCTTG GTGACTACGA GTTGGATGTC TCACCCTATG AAGACACAGT GACCAGCAAA 
CCCTGGAAAA TGAATCTTAG CAAGTTGAAC ATGCTCAAAC CAGACTCGGA CCTCTGCCTC 
AAATTTGCTA TGCTGTGTAC TCTTCACGAC AAGTGTGACC GCCTGCGCAA GGCCTACGGG 
GAGGCATGCT CAGGGATCCG CTGCCAGCGC CACCTCTGCC TAGCCCAGCT GCGCTCCTTC 
TTTGAGAAGG CAGCAGAGTC CCACGCTCAG GGTCTGCTGC TGTGTCCCTG TGCACCAGAA 
GATGCGGGCT GTGGGGAGCG GCGGCGTAAC ACCATCGCCC CCAGTTGCGC CCTGCCTTCT 
GTAACCCCCA ATTGCCTGGA TCTGCGGAGC TTCTGCCGTG CGGACCCTTT GTGCAGATCA 
CGCCTGATGG ACTTCCAGAC CCACTGTCAT CCTATGGACA TCCTTGGGAC TTGTGCAACT 
GAGCAGTCCA GATGTCTGCG GGCATACCTG GGGCTGATTG GGACTGCCAT GACCCCAAAC 
TTCATCAGCA AGGTCAACAC TACTGTTGCC TTAAGCTGCA CCTGCCGAGG CAGCGGCAAC 
CTACAGGACG AGTGTGAACA GCTGGAAAGG TCCTTCTCCC AGAACCCCTG CCTCGTGGAG 
GCCATTGCAG CTAAGATGCG TTTCCACAGA CAGCTCTTCT CCCAGGACTG GGCAGACTCT 
ACTTTTTCAG TGGTGCAGCA GCAGAACAGC AACCCTGCTC TGAGACTGCA GCCCAGGCTA 
CCCATTCTTT CTTTCTCCAT CCTTCCCTTG ATTCTGCTGC AGACCCTCTG GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Visceral motor neuron diversity delineates a cellular basis for nipple- and pilo-erection muscle control.
Nature neuroscience19(10)1331-40(2016 Oct)
Furlan A,La Manno G,L?bke M,H?ring M,Abdo H,Hochgerner H,Kupari J,Usoskin D,Airaksinen MS,Oliver G,Linnarsson S,Ernfors P

Inflammatory and neuropathic cold allodynia are selectively mediated by the neurotrophic factor receptor GFR?3.
Proceedings of the National Academy of Sciences of the United States of America113(16)4506-11(2016 Apr)
Lippoldt EK,Ongun S,Kusaka GK,McKemy DD

Hox Proteins Coordinate Motor Neuron Differentiation and Connectivity Programs through Ret/Gfr? Genes.
Cell reports14(8)1901-15(2016 Mar)
Catela C,Shin MM,Lee DH,Liu JP,Dasen JS

Expression and functional studies of the GDNF family receptor alpha 3 in the pancreas.
Journal of molecular endocrinology56(2)77-90(2016 Feb)
Nivlet L,Herrmann J,Martin DE,Meunier A,Orvain C,Gradwohl G

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Gfra3 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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