Gab2 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Gab2
Entrez Gene ID	14389
Full Name	growth factor receptor bound protein 2-associated protein 2
Synonyms	AI463667,D130058I17Rik,p97
General protein information	Preferred Names growth factor receptor bound protein 2-associated protein 2 Names GRB2-associated-binding protein 2 GRB2-associated binder 2 Grb2 associated binder 2 PH domain-containing adaptor molecule p97
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 7 Map Location: 7\|7 E1

mRNA and Protein(s)

mRNA	Protein	Name
NM_001162477.1	NP_001155949.1	GRB2-associated-binding protein 2 isoform 2
NM_010248.2	NP_034378.2	GRB2-associated-binding protein 2 isoform 1

Pathways from BioSystems

Homologies

Rattus norvegicus (Norway rat)	Gab2	NP_445869.1
Danio rerio (zebrafish)	gab2	XP_692935.3
Homo sapiens (human)	GAB2	NP_536739.1
Bos taurus (cattle)	GAB2	XP_002699116.2
Pan troglodytes (chimpanzee)	GAB2	XP_001164121.2
Canis lupus familiaris (dog)	GAB2	XP_542285.3
Gallus gallus (chicken)	GAB2	XP_001234549.1
	GAB2	XP_001093917.2
Mus musculus (house mouse)	Gab2	NP_034378.2
Xenopus tropicalis (tropical clawed frog)	gab2	XP_002936593.1

Gene Ontology
Bibliography

Related articles in PubMed

Cooperative interaction of hepatocyte growth factor and neuregulin regulates Schwann cell migration and proliferation through Grb2-associated binder-2 in peripheral nerve repair.
Shin YK, Jang SY, Yun SH, Choi YY, Yoon BA, Jo YR, Park SY, Pak MG, Park JI, Park HT
Glia65(11)1794-1808(2017 Nov)

Increased levels of Gab1 and Gab2 adaptor proteins skew interleukin-4 (IL-4) signaling toward M2 macrophage-driven pulmonary fibrosis in mice.
Guo X, Li T, Xu Y, Xu X, Zhu Z, Zhang Y, Xu J, Xu K, Cheng H, Zhang X, Ke Y
The Journal of biological chemistry292(34)14003-14015(2017 08)

Inhibition of the Gab2/PI3K/mTOR signaling ameliorates myeloid malignancy caused by Ptpn11 (Shp2) gain-of-function mutations.
Liu W, Yu WM, Zhang J, Chan RJ, Loh ML, Zhang Z, Bunting KD, Qu CK
Leukemia31(6)1415-1422(2017 Jun)

Deletion of Gab2 in mice protects against hepatic steatosis and steatohepatitis: a novel therapeutic target for fatty liver disease.
Chen S, Kang Y, Sun Y, Zhong Y, Li Y, Deng L, Tao J, Li Y, Tian Y, Zhao Y, Cheng J, Liu W, Feng GS, Lu Z
Journal of molecular cell biology8(6)492-504(2016 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Data, including data from studies in transgenic/knockout mice, suggest expression of Gab1/Gab2 is up-regulated in activated macrophages in pulmonary fibrosis; both Gab1/Gab2 are recruited to Il4r, synergistically enhancing downstream signal amplification. (Gab1 = growth factor receptor bound protein 2-associated protein 1; Gab2 = growth factor receptor bound protein 2-associated protein 2; Il4r = interleukin-4 receptor)
Title: Increased levels of Gab1 and Gab2 adaptor proteins skew interleukin-4 (IL-4) signaling toward M2 macrophage-driven pulmonary fibrosis in mice.

High Gab2 expression is associated with myeloproliferative neoplasm.
Title: Inhibition of the Gab2/PI3K/mTOR signaling ameliorates myeloid malignancy caused by Ptpn11 (Shp2) gain-of-function mutations.

Gab2 may be a disease-associated protein that is induced by pathogenic factors to amplify and coordinate multifactor-induced signals to govern disease development in the liver.
Title: Deletion of Gab2 in mice protects against hepatic steatosis and steatohepatitis: a novel therapeutic target for fatty liver disease.

Given that GAB2 is dispensable for normal hematopoiesis, GAB2 and its effectors PI3K and SHP2 represent promising targets for therapy in Ph(+)hematologic neoplasms
Title: Distinct GAB2 signaling pathways are essential for myeloid and lymphoid transformation and leukemogenesis by BCR-ABL1.

Down-regulation of Gab2 has a protective function during M. tuberculosis infection, revealing a potential negative regulatory role for Gab2 in immunity to TB.
Title: Growth factor receptor bound protein 2-associated binder 2, a scaffolding adaptor protein, negatively regulates host immunity against tuberculosis.

The following Gab2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Gab2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu08427	NM_001162477.1 Latest version!	Mus musculus growth factor receptor bound protein 2-associated protein 2 (Gab2), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$391.30 $559.00
OMu05827	NM_010248.2 Latest version!	Mus musculus growth factor receptor bound protein 2-associated protein 2 (Gab2), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Gab2 ( NM_001162477.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001155949.1 Mus musculus growth factor receptor bound protein 2-associated protein 2 (Gab2), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OMu08427
Clone ID Related Accession (Same CDS sequence)	NM_001162477.1
Accession Version	NM_001162477.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1998bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-24
Organism	Mus musculus(house mouse)
Product	GRB2-associated-binding protein 2 isoform 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC074047.6 and AC155933.3. Transcript Variant: This variant (2) uses an alternate in-frame splice site in the 3' coding region compared to variant 1. This results in a shorter protein (isoform 2) compared to isoform 1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC103524.1, AB018414.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAGCGGCG GCGGCGGCGA CGACGTGGTG TGTACCGGCT GGCTGAGGAA ATCGCCTCCC 
GAGAAGAAGT TGAGGCGCTA TGCCTGGAAG AAACGCTGGT TTATACTTCG GAGTGGCCGA 
ATGAGTGGAG ATCCAGATGT TCTGGAATAC TACAAGAATG AGCACTCCAA GAAACCCCTG 
CGGATCATCA ACCTGAACTT GTGTGAGCAG GTGGATGCAG GCCTGACCTT CAACAAGAAA 
GAGCTGCAGG ATAGTTTTGT GTTTGATATC AAGACCAGCG AGCGCACATT TTACCTGGTG 
GCTGAGACAG AGGCTGACAT GAATAAGTGG GTCCAGAGCA TCTGCCAGAT CTGCGGCTTC 
AATCAGGCTG AAGAGAGCAC AGACTCCCTG AGGAACCTTT CTTCAGCCAG TCATGGTCCC 
CGCTCTTCTC CAGCTGAGTT CAGCTCCAGT CAGCACCTGC TCCGAGAACG GAAGTCCTCA 
GCCCCTTCAC ACTCTAGCCA GCCTACTTTA TTCACGTTTG AGCCCCCTGT GTCAAGCCAC 
ATGCAGCCTA CCTTGTCCAC CAGTGCACCT CAGGAGTATC TCTACTTGCA CCAGTGCATA 
AGCAGAAGGA CAGAAAATGC AAGGAGTGCC AGCTTCTCTC AGGGCACCCG GCAGAAGAGT 
GATACAGCTG TGCAAAAACT TGCCCAGAGC AATGGACACT GTATCAACGG CGTCGGAGGT 
CAAGTCCATG GCTTCTATAG CCTTCCCAAG CCAAGCCGAC ACAATACAGA ATTCAAAGAC 
AGTACTTATG ATCTCCCACG GAGCCTGGCT TCCCATGGCC ACACCAAGAG CAGCCTCACA 
GGGTCTGAGA CTGATAACGA GGATGTGTAC ACCTTCAAGA TGCCCAGCAA CACCCTGTGT 
CGGGAACTTG GAGACCTCCT TGTGGACAAT ATGGATGTCC CAACCACTCC TCTCTCAGCC 
TACCAGATCC CTAGAACTTT CACACTGGAC AAGAACCACA ATGCCATGAC AGTGGCCACT 
CCTGGAGATT CAGCCATAGC TCCCCCACCC CGGCCACCCA AGCCAAGTCA GGCAGAAACA 
CCTCAATGGG GCAGCATTCA GCAAAGACCT CCAATCAGCG AAAATAGCAG ATCTGTAGCT 
GCTACTATCC CCAGGCGCAA TACCCTCCCT GCAATGGACA ACAGCCGACT CCATCGAGCT 
TCTTCCTGTG AGACCTACGA GTACCCGGCA CGAGGCAGTG GGGAAAGTGC CAGCTGGTCT 
GCTGAACCTC CAGGAAAGAC TGCCGTAGGT CGATCAAATA GTGCCAGCTC TGATGACAAC 
TACGTGCCCA TGAACCCAGG TTCTTCTACC CTGCTGGCTA TGGAACGACC AGGGGACAAC 
TCCCAGAGTG TCTACATCCC CATGAGCCCA GGACCCCATC ACTTTGACCC ACTTGGCTAC 
CCGTCCACAG CCCTTCCTAT TCACAGAGGC CCCAGCCGAG GAAGTGAAAT CCAGCCACCC 
CCGGTCAACC GAAACCTCAA GCCTGACAGA AAAGCAAAGC CAACACCCCT TGACCTGAGA 
AACAACACTG TCATCGATGA GCTGCCCTTC AAGTCACCTG TCACCAAGTC TTGGTCCAGG 
ATCAACCACA CCTTTAACTC CAGTTCCTCC CAGTACTGCC GTCCTATCTC CACACAGAGC 
ATCACCAGCA CAGACTCGGG AGACAGTGAG GAGAACTATG TCCCTATGCA AAACCCAGTA 
TCTGCATCCC CTGTTCCCAG TGGCACTAAC AGCCCAGCTC CAAAGAAGAG TACTGGCAGT 
GTGGATTATC TCGCCCTGGA CTTCCAGCCG GGCTCCCCAA GCCCTCACCG CAAGCCATCC 
ACATCATCTG TCACATCAGA TGAGAAGGTA GACTATGTCC AAGTGGATAA AGAGAAGACC 
CAGGCCCTGC AGAACACCAT GCAGGAGTGG ACAGATGTGC GGCAGTCCTC CGAACCTTCC 
AAGGGTGCCA AGCTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001155949.1
CDS	76..2073
Translation	MSGGGGDDVVCTGWLRKSPPEKKLRRYAWKKRWFILRSGRMSGDPDVLEYYKNEHSKKPLRIINLNLCEQVDAGLTFNKKELQDSFVFDIKTSERTFYLVAETEADMNKWVQSICQICGFNQAEESTDSLRNLSSASHGPRSSPAEFSSSQHLLRERKSSAPSHSSQPTLFTFEPPVSSHMQPTLSTSAPQEYLYLHQCISRRTENARSASFSQGTRQKSDTAVQKLAQSNGHCINGVGGQVHGFYSLPKPSRHNTEFKDSTYDLPRSLASHGHTKSSLTGSETDNEDVYTFKMPSNTLCRELGDLLVDNMDVPTTPLSAYQIPRTFTLDKNHNAMTVATPGDSAIAPPPRPPKPSQAETPQWGSIQQRPPISENSRSVAATIPRRNTLPAMDNSRLHRASSCETYEYPARGSGESASWSAEPPGKTAVGRSNSASSDDNYVPMNPGSSTLLAMERPGDNSQSVYIPMSPGPHHFDPLGYPSTALPIHRGPSRGSEIQPPPVNRNLKPDRKAKPTPLDLRNNTVIDELPFKSPVTKSWSRINHTFNSSSSQYCRPISTQSITSTDSGDSEENYVPMQNPVSASPVPSGTNSPAPKKSTGSVDYLALDFQPGSPSPHRKPSTSSVTSDEKVDYVQVDKEKTQALQNTMQEWTDVRQSSEPSKGAKL

Target ORF information:

RefSeq Version	NM_001162477.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus growth factor receptor bound protein 2-associated protein 2 (Gab2), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001162477.1

ORF Insert Sequence:

ATGAGCGGCG GCGGCGGCGA CGACGTGGTG TGTACCGGCT GGCTGAGGAA ATCGCCTCCC 
GAGAAGAAGT TGAGGCGCTA TGCCTGGAAG AAACGCTGGT TTATACTTCG GAGTGGCCGA 
ATGAGTGGAG ATCCAGATGT TCTGGAATAC TACAAGAATG AGCACTCCAA GAAACCCCTG 
CGGATCATCA ACCTGAACTT GTGTGAGCAG GTGGATGCAG GCCTGACCTT CAACAAGAAA 
GAGCTGCAGG ATAGTTTTGT GTTTGATATC AAGACCAGCG AGCGCACATT TTACCTGGTG 
GCTGAGACAG AGGCTGACAT GAATAAGTGG GTCCAGAGCA TCTGCCAGAT CTGCGGCTTC 
AATCAGGCTG AAGAGAGCAC AGACTCCCTG AGGAACCTTT CTTCAGCCAG TCATGGTCCC 
CGCTCTTCTC CAGCTGAGTT CAGCTCCAGT CAGCACCTGC TCCGAGAACG GAAGTCCTCA 
GCCCCTTCAC ACTCTAGCCA GCCTACTTTA TTCACGTTTG AGCCCCCTGT GTCAAGCCAC 
ATGCAGCCTA CCTTGTCCAC CAGTGCACCT CAGGAGTATC TCTACTTGCA CCAGTGCATA 
AGCAGAAGGA CAGAAAATGC AAGGAGTGCC AGCTTCTCTC AGGGCACCCG GCAGAAGAGT 
GATACAGCTG TGCAAAAACT TGCCCAGAGC AATGGACACT GTATCAACGG CGTCGGAGGT 
CAAGTCCATG GCTTCTATAG CCTTCCCAAG CCAAGCCGAC ACAATACAGA ATTCAAAGAC 
AGTACTTATG ATCTCCCACG GAGCCTGGCT TCCCATGGCC ACACCAAGAG CAGCCTCACA 
GGGTCTGAGA CTGATAACGA GGATGTGTAC ACCTTCAAGA TGCCCAGCAA CACCCTGTGT 
CGGGAACTTG GAGACCTCCT TGTGGACAAT ATGGATGTCC CAACCACTCC TCTCTCAGCC 
TACCAGATCC CTAGAACTTT CACACTGGAC AAGAACCACA ATGCCATGAC AGTGGCCACT 
CCTGGAGATT CAGCCATAGC TCCCCCACCC CGGCCACCCA AGCCAAGTCA GGCAGAAACA 
CCTCAATGGG GCAGCATTCA GCAAAGACCT CCAATCAGCG AAAATAGCAG ATCTGTAGCT 
GCTACTATCC CCAGGCGCAA TACCCTCCCT GCAATGGACA ACAGCCGACT CCATCGAGCT 
TCTTCCTGTG AGACCTACGA GTACCCGGCA CGAGGCAGTG GGGAAAGTGC CAGCTGGTCT 
GCTGAACCTC CAGGAAAGAC TGCCGTAGGT CGATCAAATA GTGCCAGCTC TGATGACAAC 
TACGTGCCCA TGAACCCAGG TTCTTCTACC CTGCTGGCTA TGGAACGACC AGGGGACAAC 
TCCCAGAGTG TCTACATCCC CATGAGCCCA GGACCCCATC ACTTTGACCC ACTTGGCTAC 
CCGTCCACAG CCCTTCCTAT TCACAGAGGC CCCAGCCGAG GAAGTGAAAT CCAGCCACCC 
CCGGTCAACC GAAACCTCAA GCCTGACAGA AAAGCAAAGC CAACACCCCT TGACCTGAGA 
AACAACACTG TCATCGATGA GCTGCCCTTC AAGTCACCTG TCACCAAGTC TTGGTCCAGG 
ATCAACCACA CCTTTAACTC CAGTTCCTCC CAGTACTGCC GTCCTATCTC CACACAGAGC 
ATCACCAGCA CAGACTCGGG AGACAGTGAG GAGAACTATG TCCCTATGCA AAACCCAGTA 
TCTGCATCCC CTGTTCCCAG TGGCACTAAC AGCCCAGCTC CAAAGAAGAG TACTGGCAGT 
GTGGATTATC TCGCCCTGGA CTTCCAGCCG GGCTCCCCAA GCCCTCACCG CAAGCCATCC 
ACATCATCTG TCACATCAGA TGAGAAGGTA GACTATGTCC AAGTGGATAA AGAGAAGACC 
CAGGCCCTGC AGAACACCAT GCAGGAGTGG ACAGATGTGC GGCAGTCCTC CGAACCTTCC 
AAGGGTGCCA AGCTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gab2 ( NM_010248.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_034378.2 Mus musculus growth factor receptor bound protein 2-associated protein 2 (Gab2), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OMu05827
Clone ID Related Accession (Same CDS sequence)	NM_010248.2
Accession Version	NM_010248.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2001bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-24
Organism	Mus musculus(house mouse)
Product	GRB2-associated-binding protein 2 isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC074047.6 and AC155933.3. On Jul 2, 2009 this sequence version replaced NM_010248.1. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF104244.1, BC103525.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAGCGGCG GCGGCGGCGA CGACGTGGTG TGTACCGGCT GGCTGAGGAA ATCGCCTCCC 
GAGAAGAAGT TGAGGCGCTA TGCCTGGAAG AAACGCTGGT TTATACTTCG GAGTGGCCGA 
ATGAGTGGAG ATCCAGATGT TCTGGAATAC TACAAGAATG AGCACTCCAA GAAACCCCTG 
CGGATCATCA ACCTGAACTT GTGTGAGCAG GTGGATGCAG GCCTGACCTT CAACAAGAAA 
GAGCTGCAGG ATAGTTTTGT GTTTGATATC AAGACCAGCG AGCGCACATT TTACCTGGTG 
GCTGAGACAG AGGCTGACAT GAATAAGTGG GTCCAGAGCA TCTGCCAGAT CTGCGGCTTC 
AATCAGGCTG AAGAGAGCAC AGACTCCCTG AGGAACCTTT CTTCAGCCAG TCATGGTCCC 
CGCTCTTCTC CAGCTGAGTT CAGCTCCAGT CAGCACCTGC TCCGAGAACG GAAGTCCTCA 
GCCCCTTCAC ACTCTAGCCA GCCTACTTTA TTCACGTTTG AGCCCCCTGT GTCAAGCCAC 
ATGCAGCCTA CCTTGTCCAC CAGTGCACCT CAGGAGTATC TCTACTTGCA CCAGTGCATA 
AGCAGAAGGA CAGAAAATGC AAGGAGTGCC AGCTTCTCTC AGGGCACCCG GCAGAAGAGT 
GATACAGCTG TGCAAAAACT TGCCCAGAGC AATGGACACT GTATCAACGG CGTCGGAGGT 
CAAGTCCATG GCTTCTATAG CCTTCCCAAG CCAAGCCGAC ACAATACAGA ATTCAAAGAC 
AGTACTTATG ATCTCCCACG GAGCCTGGCT TCCCATGGCC ACACCAAGAG CAGCCTCACA 
GGGTCTGAGA CTGATAACGA GGATGTGTAC ACCTTCAAGA TGCCCAGCAA CACCCTGTGT 
CGGGAACTTG GAGACCTCCT TGTGGACAAT ATGGATGTCC CAACCACTCC TCTCTCAGCC 
TACCAGATCC CTAGAACTTT CACACTGGAC AAGAACCACA ATGCCATGAC AGTGGCCACT 
CCTGGAGATT CAGCCATAGC TCCCCCACCC CGGCCACCCA AGCCAAGTCA GGCAGAAACA 
CCTCAATGGG GCAGCATTCA GCAAAGACCT CCAATCAGCG AAAATAGCAG ATCTGTAGCT 
GCTACTATCC CCAGGCGCAA TACCCTCCCT GCAATGGACA ACAGCCGACT CCATCGAGCT 
TCTTCCTGTG AGACCTACGA GTACCCGGCA CGAGGCAGTG GGGAAAGTGC CAGCTGGTCT 
GCTGAACCTC CAGGAAAGAC TGCCGTAGGT CGATCAAATA GTGCCAGCTC TGATGACAAC 
TACGTGCCCA TGAACCCAGG TTCTTCTACC CTGCTGGCTA TGGAACGACC AGGGGACAAC 
TCCCAGAGTG TCTACATCCC CATGAGCCCA GGACCCCATC ACTTTGACCC ACTTGGCTAC 
CCGTCCACAG CCCTTCCTAT TCACAGAGGC CCCAGCCGAG GAAGTGAAAT CCAGCCACCC 
CCGGTCAACC GAAACCTCAA GCCTGACAGA AAAGCAAAGC CAACACCCCT TGACCTGAGA 
AACAACACTG TCATCGATGA GCTGCCCTTC AAGTCACCTG TCACCAAGTC TTGGTCCAGG 
ATCAACAGCC ACACCTTTAA CTCCAGTTCC TCCCAGTACT GCCGTCCTAT CTCCACACAG 
AGCATCACCA GCACAGACTC GGGAGACAGT GAGGAGAACT ATGTCCCTAT GCAAAACCCA 
GTATCTGCAT CCCCTGTTCC CAGTGGCACT AACAGCCCAG CTCCAAAGAA GAGTACTGGC 
AGTGTGGATT ATCTCGCCCT GGACTTCCAG CCGGGCTCCC CAAGCCCTCA CCGCAAGCCA 
TCCACATCAT CTGTCACATC AGATGAGAAG GTAGACTATG TCCAAGTGGA TAAAGAGAAG 
ACCCAGGCCC TGCAGAACAC CATGCAGGAG TGGACAGATG TGCGGCAGTC CTCCGAACCT 
TCCAAGGGTG CCAAGCTGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_034378.2
CDS	76..2076
Translation	MSGGGGDDVVCTGWLRKSPPEKKLRRYAWKKRWFILRSGRMSGDPDVLEYYKNEHSKKPLRIINLNLCEQVDAGLTFNKKELQDSFVFDIKTSERTFYLVAETEADMNKWVQSICQICGFNQAEESTDSLRNLSSASHGPRSSPAEFSSSQHLLRERKSSAPSHSSQPTLFTFEPPVSSHMQPTLSTSAPQEYLYLHQCISRRTENARSASFSQGTRQKSDTAVQKLAQSNGHCINGVGGQVHGFYSLPKPSRHNTEFKDSTYDLPRSLASHGHTKSSLTGSETDNEDVYTFKMPSNTLCRELGDLLVDNMDVPTTPLSAYQIPRTFTLDKNHNAMTVATPGDSAIAPPPRPPKPSQAETPQWGSIQQRPPISENSRSVAATIPRRNTLPAMDNSRLHRASSCETYEYPARGSGESASWSAEPPGKTAVGRSNSASSDDNYVPMNPGSSTLLAMERPGDNSQSVYIPMSPGPHHFDPLGYPSTALPIHRGPSRGSEIQPPPVNRNLKPDRKAKPTPLDLRNNTVIDELPFKSPVTKSWSRINSHTFNSSSSQYCRPISTQSITSTDSGDSEENYVPMQNPVSASPVPSGTNSPAPKKSTGSVDYLALDFQPGSPSPHRKPSTSSVTSDEKVDYVQVDKEKTQALQNTMQEWTDVRQSSEPSKGAKL

Target ORF information:

RefSeq Version	NM_010248.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus growth factor receptor bound protein 2-associated protein 2 (Gab2), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_010248.2

ORF Insert Sequence:

ATGAGCGGCG GCGGCGGCGA CGACGTGGTG TGTACCGGCT GGCTGAGGAA ATCGCCTCCC 
GAGAAGAAGT TGAGGCGCTA TGCCTGGAAG AAACGCTGGT TTATACTTCG GAGTGGCCGA 
ATGAGTGGAG ATCCAGATGT TCTGGAATAC TACAAGAATG AGCACTCCAA GAAACCCCTG 
CGGATCATCA ACCTGAACTT GTGTGAGCAG GTGGATGCAG GCCTGACCTT CAACAAGAAA 
GAGCTGCAGG ATAGTTTTGT GTTTGATATC AAGACCAGCG AGCGCACATT TTACCTGGTG 
GCTGAGACAG AGGCTGACAT GAATAAGTGG GTCCAGAGCA TCTGCCAGAT CTGCGGCTTC 
AATCAGGCTG AAGAGAGCAC AGACTCCCTG AGGAACCTTT CTTCAGCCAG TCATGGTCCC 
CGCTCTTCTC CAGCTGAGTT CAGCTCCAGT CAGCACCTGC TCCGAGAACG GAAGTCCTCA 
GCCCCTTCAC ACTCTAGCCA GCCTACTTTA TTCACGTTTG AGCCCCCTGT GTCAAGCCAC 
ATGCAGCCTA CCTTGTCCAC CAGTGCACCT CAGGAGTATC TCTACTTGCA CCAGTGCATA 
AGCAGAAGGA CAGAAAATGC AAGGAGTGCC AGCTTCTCTC AGGGCACCCG GCAGAAGAGT 
GATACAGCTG TGCAAAAACT TGCCCAGAGC AATGGACACT GTATCAACGG CGTCGGAGGT 
CAAGTCCATG GCTTCTATAG CCTTCCCAAG CCAAGCCGAC ACAATACAGA ATTCAAAGAC 
AGTACTTATG ATCTCCCACG GAGCCTGGCT TCCCATGGCC ACACCAAGAG CAGCCTCACA 
GGGTCTGAGA CTGATAACGA GGATGTGTAC ACCTTCAAGA TGCCCAGCAA CACCCTGTGT 
CGGGAACTTG GAGACCTCCT TGTGGACAAT ATGGATGTCC CAACCACTCC TCTCTCAGCC 
TACCAGATCC CTAGAACTTT CACACTGGAC AAGAACCACA ATGCCATGAC AGTGGCCACT 
CCTGGAGATT CAGCCATAGC TCCCCCACCC CGGCCACCCA AGCCAAGTCA GGCAGAAACA 
CCTCAATGGG GCAGCATTCA GCAAAGACCT CCAATCAGCG AAAATAGCAG ATCTGTAGCT 
GCTACTATCC CCAGGCGCAA TACCCTCCCT GCAATGGACA ACAGCCGACT CCATCGAGCT 
TCTTCCTGTG AGACCTACGA GTACCCGGCA CGAGGCAGTG GGGAAAGTGC CAGCTGGTCT 
GCTGAACCTC CAGGAAAGAC TGCCGTAGGT CGATCAAATA GTGCCAGCTC TGATGACAAC 
TACGTGCCCA TGAACCCAGG TTCTTCTACC CTGCTGGCTA TGGAACGACC AGGGGACAAC 
TCCCAGAGTG TCTACATCCC CATGAGCCCA GGACCCCATC ACTTTGACCC ACTTGGCTAC 
CCGTCCACAG CCCTTCCTAT TCACAGAGGC CCCAGCCGAG GAAGTGAAAT CCAGCCACCC 
CCGGTCAACC GAAACCTCAA GCCTGACAGA AAAGCAAAGC CAACACCCCT TGACCTGAGA 
AACAACACTG TCATCGATGA GCTGCCCTTC AAGTCACCTG TCACCAAGTC TTGGTCCAGG 
ATCAACAGCC ACACCTTTAA CTCCAGTTCC TCCCAGTACT GCCGTCCTAT CTCCACACAG 
AGCATCACCA GCACAGACTC GGGAGACAGT GAGGAGAACT ATGTCCCTAT GCAAAACCCA 
GTATCTGCAT CCCCTGTTCC CAGTGGCACT AACAGCCCAG CTCCAAAGAA GAGTACTGGC 
AGTGTGGATT ATCTCGCCCT GGACTTCCAG CCGGGCTCCC CAAGCCCTCA CCGCAAGCCA 
TCCACATCAT CTGTCACATC AGATGAGAAG GTAGACTATG TCCAAGTGGA TAAAGAGAAG 
ACCCAGGCCC TGCAGAACAC CATGCAGGAG TGGACAGATG TGCGGCAGTC CTCCGAACCT 
TCCAAGGGTG CCAAGCTGTA A