Fn1 cDNA ORF clone, Mus musculus(house mouse)

The following Fn1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Fn1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OMu27265 XM_006495697.4
Latest version!
Mus musculus fibronectin 1 (Fn1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1301.30
$1859.00
OMu27267 XM_006495699.4
Latest version!
Mus musculus fibronectin 1 (Fn1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1301.30
$1859.00
OMu27268 XM_006495700.4
Latest version!
Mus musculus fibronectin 1 (Fn1), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1301.30
$1859.00
OMu76976 XM_017315775.2
Latest version!
Mus musculus fibronectin 1 (Fn1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1301.30
$1859.00
OMu76977 XM_017315837.2
Latest version!
Mus musculus fibronectin 1 (Fn1), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1091.30
$1559.00
OMu76978 XM_017315848.2
Latest version!
Mus musculus fibronectin 1 (Fn1), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1091.30
$1559.00
OMu85006 XM_030248098.1
Latest version!
Mus musculus fibronectin 1 (Fn1), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1301.30
$1859.00
OMu85007 XM_030248135.1
Latest version!
Mus musculus fibronectin 1 (Fn1), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1091.30
$1559.00
OMu17493 NM_001276409.1
Latest version!
Mus musculus fibronectin 1 (Fn1), transcript variant 3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1301.30
$1859.00
OMu17547 NM_001276408.1
Latest version!
Mus musculus fibronectin 1 (Fn1), transcript variant 2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1301.30
$1859.00
OMu17657 NM_010233.2
Latest version!
Mus musculus fibronectin 1 (Fn1), transcript variant 1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1301.30
$1859.00
OMu23089 NM_001276410.1
Latest version!
Mus musculus fibronectin 1 (Fn1), transcript variant 4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1091.30
$1559.00
OMu23188 NM_001276411.1
Latest version!
Mus musculus fibronectin 1 (Fn1), transcript variant 5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1091.30
$1559.00
OMu23220 NM_001276412.1
Latest version!
Mus musculus fibronectin 1 (Fn1), transcript variant 6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1091.30
$1559.00
OMu23284 NM_001276413.1
Latest version!
Mus musculus fibronectin 1 (Fn1), transcript variant 7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1091.30
$1559.00
View Old Accession Versions >>
OMu27265 XM_006495697.3 Mus musculus fibronectin 1 (Fn1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1301.30
$1859.00
OMu27267 XM_006495699.3 Mus musculus fibronectin 1 (Fn1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1301.30
$1859.00
OMu76976 XM_017315775.1 Mus musculus fibronectin 1 (Fn1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1301.30
$1859.00
OMu76977 XM_017315837.1 Mus musculus fibronectin 1 (Fn1), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1091.30
$1559.00
OMu76978 XM_017315848.1 Mus musculus fibronectin 1 (Fn1), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1091.30
$1559.00
OMu27268 XM_006495700.3 Mus musculus fibronectin 1 (Fn1), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1301.30
$1859.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OMu27265
    Clone ID Related Accession (Same CDS sequence) XM_006495697.3 , XM_006495697.4
    Accession Version XM_006495697.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7359bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product fibronectin isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006495697.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    ATGCTCAGGG GTCCGGGACC CGGGCGGCTG CTGCTGCTGG CAGTCCTGTG CCTGGGGACC 
    TCGGTGCGCT GCACCGAAGC CGGGAAGAGC AAGAGGCAGG CTCAGCAAAT CGTGCAGCCT
    CAATCCCCGG TGGCTGTCAG TCAGAGCAAG CCTGGCTGTT TTGACAATGG GAAGCACTAT
    CAGATAAATC AGCAGTGGGA ACGGACCTAC CTAGGCAACG CCCTGGTTTG TACCTGCTAT
    GGAGGAAGCC GGGGTTTTAA CTGCGAGAGC AAGCCTGAGC CTGAAGAGAC TTGCTTTGAC
    AAATACACTG GGAACACTTA CAAAGTGGGT GACACTTATG AGCGCCCTAA AGATTCCATG
    ATCTGGGACT GTACCTGCAT CGGGGCTGGG AGAGGCAGGA TCAGCTGTAC CATTGCAAAT
    CGCTGCCATG AAGGGGGTCA GTCCTACAAG ATTGGCGACA AGTGGAGGAG GCCACATGAG
    ACTGGTGGCT ACATGTTAGA GTGTCTGTGT CTGGGAAATG GAAAAGGGGA ATGGACCTGC
    AAACCTATAG CTGAGAAGTG TTTTGATCAT GCTGCTGGGA CGTCCTACGT CGTGGGGGAG
    ACCTGGGAAA AGCCCTACCA AGGCTGGATG ATGGTGGACT GTACTTGTCT AGGCGAAGGC
    AATGGACGCA TCACCTGTAC CTCCAGAAAC AGATGCAACG ATCAGGACAC CCGGACATCC
    TATAGGATTG GAGACACGTG GAGCAAGAAG GACAACCGAG GAAACCTGCT TCAGTGTGTC
    TGCACAGGCA ATGGCAGAGG GGAGTGGAAG TGTGAGCGAC ATGCTCTACA AAGTGCTTCA
    GCCGGATCTG GCTCCTTCAC TGATGTCCGA ACAGCTATTT ACCAACCGCA GACTCACCCC
    CAGCCCGCTC CCTACGGCCA CTGTGTCACC GACAGTGGTG TGGTCTACTC TGTGGGAATG
    CAGTGGCTGA AGTCGCAAGG AAACAAGCAA ATGCTGTGCA CGTGCCTGGG CAATGGCGTC
    AGCTGCCAGG AGACAGCCGT GACCCAGACT TATGGTGGCA ATTCAAACGG GGAGCCCTGT
    GTCCTCCCGT TCACCTACAA CGGTAGGACC TTCTATTCCT GCACCACCGA AGGGCGGCAA
    GACGGACATC TGTGGTGTAG CACAACTTCC AATTACGAAC AAGACCAGAA GTATTCCTTC
    TGCACAGACC ATGCGGTTTT GGTTCAGACT CGAGGCGGAA ATTCCAATGG TGCTCTGTGC
    CACTTCCCCT TCCTGTACAA CAACCGGAAT TACACCGACT GTACTTCTGA GGGTCGCAGG
    GACAACATGA AATGGTGCGG CACCACCCAG AACTACGATG CCGATCAGAA GTTTGGATTC
    TGCCCAATGG CTGCCCACGA GGAGATCTGC ACAACCAATG AAGGGGTCAT GTATCGCATT
    GGGGATCAGT GGGATAAGCA GCATGACCTG GGCCACATGA TGAGGTGCAC GTGTGTGGGG
    AACGGTCGTG GAGAATGGGC CTGCATCCCC TACTCCCAGC TCCGAGACCA GTGCATCGTT
    GATGACATTA CTTACAATGT GAACGACACG TTCCACAAGC GTCACGAGGA GGGACATATG
    CTGAACTGTA CCTGCTTTGG TCAGGGCCGG GGCAGATGGA AGTGTGACCC CATTGACCAG
    TGCCAAGATT CAGAGACCCG GACATTTTAC CAGATTGGTG ACTCCTGGGA GAAGTTTGTG
    CATGGTGTCC GATACCAGTG TTACTGCTAC GGCCGTGGCA TCGGGGAGTG GCACTGTCAA
    CCTCTGCAGA CCTACCCAGG CACAACTGGA CCTGTCCAAG TAATTATCAC GGAGACCCCC
    AGCCAGCCCA ATTCCCACCC CATCCAGTGG AATGCCCCGG AGCCTTCACA CATCACCAAG
    TACATTCTCA GATGGAGACC TAAAACCTCT ACGGGTCGCT GGAAGGAAGC TACCATTCCT
    GGCCACCTTA ACTCCTATAC CATCAAAGGC CTGACCCCAG GTGTGATCTA TGAGGGACAG
    CTCATCAGCA TCCAGCAGTA TGGCCACAGA GAAGTGACAC GCTTCGACTT CACCACCAGC
    GCCAGCACCC CTGTGACCAG CAACACGGTG ACCGGAGAGA CTGCGCCCTA CTCTCCTGTT
    GTGGCCACTT CTGAATCTGT AACTGAAATC ACAGCCAGCA GCTTTGTGGT CTCATGGGTC
    TCAGCCTCCG ACACCGTGTC AGGCTTCCGG GTGGAGTATG AGCTGAGCGA GGAGGGAGAT
    GAACCACAGT ACCTTGATCT CCCAAGCACG GCCACTTCCG TGAACATTCC TGACCTGCTC
    CCGGGCAGAA AGTACATTGT CAATGTCTAT CAGATATCTG AGGAGGGAAA ACAGAGCTTG
    ATCCTGTCTA CCTCACAGAC TACAGCACCT GACGCTCCTC CAGACCCTAC CGTGGACCAG
    GTTGATGATA CTTCCATTGT TGTTCGGTGG AGTAGACCCC AGGCACCTAT CACAGGGTAT
    AGAATTGTCT ATTCACCTTC AGTAGAAGGC AGTAGCACAG AGCTCAACCT CCCTGAAACG
    GCCAACTCCG TCACCCTCAG CGACCTGCAG CCCGGTGTTC AGTACAACAT CACTATCTAT
    GCTGTGGAGG AGAACCAGGA GAGCACACCC GTTTTCATCC AACAAGAGAC CACTGGCACC
    CCAAGATCTG ATAACGTCCC CCCTCCGACG GACCTACAGT TTGTGGAACT GACTGATGTG
    AAAGTCACCA TCATGTGGAC CCCTCCTGAT AGTGTGGTGT CTGGATACCG TGTGGAGGTC
    CTGCCTGTCA GCCTGCCCGG GGAACATGGG CAGAGGCTGC CTGTCAACAG AAATACCTTT
    GCTGAAATCA CTGGGCTGTC CCCTGGGGTC ACGTACCTCT TCAAAGTCTT TGCTGTGCAC
    CAGGGCAGGG AAAGCAATCC TCTGACGGCA CAACAGACCA CCAAACTCGA CGCTCCCACT
    AACCTCCAGT TTGTCAATGA AACTGACAGA ACAGTTCTGG TAACGTGGAC TCCACCTCGA
    GCCCGTATAG CAGGCTACCG ACTGACCGCG GGCCTGACCC GAGGAGGCCA GCCCAAGCAG
    TACAATGTGG GACCCTTGGC CTCCAAGTAT CCCCTGAGAA ATCTGCAGCC TGGGTCTGAG
    TACACCGTGA CCTTGGTGGC TGTGAAAGGG AACCAGCAGA GTCCCAAAGC CACCGGAGTC
    TTTACTACCC TGCAGCCTCT GCGCTCCATT CCACCTTACA ACACCGAGGT GACAGAGACC
    ACAATTGTGA TCACCTGGAC CCCCGCTCCA AGGATTGGCT TCAAGCTGGG TGTACGACCG
    AGCCAGGGAG GTGAGGCACC CCGAGAAGTG ACTTCAGACT CTGGGAGCAT TGTTGTGTCT
    GGCTTGACTC CAGGCGTGGA ATACACTTAC ACCATCCAAG TCCTGCGAGA TGGCCAGGAG
    AGAGATGCAC CGATTGTCAA CAGAGTAGTG ACACCGCTGT CTCCACCGAC CAACTTGCAT
    CTGGAGGCAA ACCCTGACAC TGGAGTGCTT ACTGTCTCCT GGGAGAGGAG CACTACCCCA
    GATATCACTG GCTACAGAAT AACTACTACC CCCACGAACG GGCAGCAGGG GACCTCTCTG
    GAAGAAGTGG TCCATGCTGA TCAGAGTTCC TGCACTTTTG AGAACCTGAA TCCTGGCCTG
    GAGTACAACG TCAGTGTTTA CACTGTCAAA GATGACAAGG AAAGTGCCCC TATCTCTGAT
    ACCGTTGTCC CAGAGGTGCC CCAGCTCACT GACCTAAGCT TTGTTGATAT AACTGATTCA
    AGCATCGGCC TGAGGTGGAC CCCGCTAAAC TCTTCCACCA TTATCGGGTA CCGAATCACA
    GTAGTTGCGG CAGGAGAAGG GATCCCTATT TTTGAAGATT TTGTGGACTC CTCAGTAGGA
    TACTACACAG TTACAGGGCT GGAGCCTGGC ATTGACTATG ACATCAGCGT TATCACTCTC
    ATTAATGGCG GAGAGAGTGC CCCTACTACA CTGACACAGC AAACGGCTGT CCCTCCTCCC
    ACGGATCTGC GATTCACCAA TATCGGTCCA GACACGATGC GGGTCACTTG GGCCCCGCCT
    CCGTCCATCG AGCTGACCAA CCTCTTGGTG CGCTACTCAC CCGTGAAGAA TGAAGAGGAC
    GTTGCAGAGC TATCCATTTC ACCTTCAGAC AATGCCGTGG TCCTAACAAA TCTCCTGCCT
    GGGACAGAAT ACTTAGTCAG TGTCTCCAGT GTCTACGAAC AACATGAGAG CATCCCTCTC
    CGGGGAAGGC AGAAAACAGG TCTCGATTCC CCAACTGGTT TTGATTCTTC TGATATCACC
    GCCAACTCAT TCACTGTCCA CTGGGTGGCT CCTCGGGCCC CCATCACCGG CTACATCATC
    CGCCATCACG CCGAGCATTC TGTCGGAAGA CCCAGGCAGG ATCGAGTGCC GCCCTCGCGG
    AATTCCATCA CCCTCACCAA CCTTAATCCG GGCACCGAGT ACGTTGTCAG CATCATTGCT
    GTTAATGGCA GAGAGGAGAG CCCGCCACTG ATTGGCCAGC AAGCCACAGT TTCTGATATT
    CCGAGAGATC TGGAGGTCAT TGCCTCCACC CCCACCAGCC TGCTCATCAG TTGGGAACCC
    CCTGCCGTCT CTGTGCGCTA TTACAGAATC ACCTACGGAG AGACAGGAGG AAATAGCCCT
    GTCCAGGAGT TCACTGTGCC CGGAAGCAAG TCCACAGCCA CCATCAACAA CATTAAACCA
    GGAGCAGACT ACACCATCAC CCTGTATGCT GTCACTGGCC GTGGGGACAG TCCAGCAAGC
    AGCAAGCCAG TTTCCATCAA TTATAAAACA GAAATTGACA AGCCGTCCCA GATGCAGGTG
    ACAGATGTCC AGGACAACAG CATCAGTGTC AGGTGGCTGC CTTCAACTTC TCCTGTGACA
    GGCTACAGAG TGACCACCAC TCCCAAAAAT GGCCTAGGAC CATCAAAAAC TAAAACTGCC
    AGTCCAGATC AAACAGAAAT GACCATTGAA GGTTTGCAAC CCACTGTGGA GTACGTGGTT
    AGTGTTTATG CTCAGAACCG GAACGGAGAA AGCCAGCCCC TGGTTCAAAC TGCAGTGACC
    AACATTGATC GCCCTAAAGG ACTGGCATTC ACTGATGTGG ATGTCGATTC CATCAAAATT
    GCTTGGGAAA GCCCACAGGG GCAAGTTTCC AGGTACAGGG TGACCTACTC GAGCCCTGAG
    GATGGAATCC GGGAGCTTTT CCCTGCACCT GATGGTGAAG ACGACACTGC AGAGCTGCAG
    GGCCTCAGGC CGGGGTCTGA GTACACAGTC AGTGTGGTTG CCTTGCACGA TGATATGGAG
    AGCCAGCCCC TGATTGGAAT CCAGTCCACA GCCATTCCTG CGCCCACCAA TCTGAAGTTC
    AGTCAGGTGA CACCCACCAG CTTTACTGCC CAGTGGATAG CACCCAGTGT TCAGCTCACT
    GGCTACCGGG TGCGGGTGAA CCCGAAAGAG AAGACAGGAC CAATGAAAGA AATCAACCTT
    TCTCCAGACA GCTCATCGGT GATTGTGTCA GGACTCATGG TGGCCACTAA ATACGAAGTC
    AGTGTCTATG CTCTCAAGGA CACACTGACA AGCAGACCAG CCCAGGGAGT CATCACTACT
    CTGGAGAATG TTAGCCCTCC AAGAAGGGCC CGTGTGACGG ACGCTACAGA GACCACCATC
    ACTATTAGCT GGAGAACAAA GACAGAGACA ATCACTGGCT TCCAAGTCGA TGCCATCCCA
    GCCAATGGCC AGACCCCAGT TCAGAGGAGC ATCAGCCCGG ATGTTAGAAG CTACACCATT
    ACAGGTTTAC AGCCAGGCAC TGACTACAAG ATCCACCTGT ACACTCTGAA CGACAATGCC
    CGGAGCTCGC CCGTGATCAT CGATGCCTCC ACCGCCATCG ACGCACCATC CAACCTGCGG
    TTCCTGACCA CCACACCCAA CTCCTTGCTG GTGTCATGGC AGGCGCCCCG TGCCAGGATT
    ACTGGCTACA TTATCAAGTA TGAGAAGCCT GGATCCCCTC CCAGAGAAGT GGTCCCTCGG
    CCCCGCCCTG GTGTCACGGA GGCCACCATT ACTGGTCTGG AGCCAGGAAC CGAGTACACC
    ATCTACGTCA TTGCCCTGAA GAACAATCAG AAGAGTGAGC CCCTGATTGG GAGGAAGAAG
    ACAGTTCAAA AGACCCCCTT CATCACCAAC CCTGGGTATG ACACCGAAAA TGGTATTCAG
    CTTCCTGGCA CAACCCACCA GCAACCCAGT GTTGGGCAAC AAATGATCTT TGAGGAACAT
    GGCTTTAGGC GGACAACGCC ACCCACTGCG GCCACCCCCG TCAGGCTTAG GCCAAGACCA
    TACCTGCCGA ATGTAGATGA GGAGGTCCAA ATCGGTCATG TTCCCAGGGG AGACGTAGAC
    TACCACCTCT ATCCTCATGT TCCGGGCCTC AATCCAAATG CCTCTACAGG ACAGGAAGCT
    CTCTCTCAGA CAACCATCTC TTGGACGCCG TTCCAGGAGA GTTCTGAGTA CATCATTTCA
    TGCCAACCAG TTGGCACCGA CGAAGAGCCC TTACAGTTCC AAGTTCCTGG AACTTCTACC
    AGTGCGACTC TGACTGGCCT TACCAGAGGG GTCACCTACA ACATCATAGT GGAGGCACTG
    CAGAACCAGA GGAGGCACAA GGTTCGGGAA GAGGTTGTGA CTGTGGGCAA CGCTGTCAGC
    GAAGGCCTGA ACCAGCCTAC AGATGACTCA TGCTTTGACC CTTACACGGT TTCCCATTAC
    GCCATTGGAG AGGAGTGGGA GCGGTTGTCT GACGCTGGCT TTAAGCTCAC ATGCCAGTGC
    TTGGGCTTTG GCAGTGGTCA TTTCAGATGC GATTCATCTA AATGGTGCCA TGACAACGGT
    GTCAACTACA AGATCGGAGA GAAGTGGGAT CGGCAGGGAG AAAATGGCCA GCGGATGAGC
    TGCACATGCC TCGGGAATGG AAAGGGAGAA TTCAAGTGTG ATCCCCATGA AGCAACGTGC
    TATGACGATG GGAAGACCTA CCATGTAGGA GAACAGTGGC AGAAAGAATA TCTCGGAGCC
    ATTTGCTCCT GCACGTGTTT CGGAGGCCAG CGGGGCTGGC GCTGTGACAA CTGCCGTAGA
    CCTGGGGCTG CTGAACCCAG TCCCGATGGC ACCACCGGCC ACACCTACAA CCAGTATACA
    CAGAGATACA ATCAGAGAAC AAACACTAAC GTAAATTGCC CCATTGAGTG CTTCATGCCG
    CTAGATGTGC AAGCTGACAG AGACGATTCT CGAGAGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006495760.1
    CDS299..7657
    Translation

    Target ORF information:

    RefSeq Version XM_006495697.4
    Organism Mus musculus(house mouse)
    Definition Mus musculus fibronectin 1 (Fn1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006495697.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    ATGCTCAGGG GTCCGGGACC CGGGCGGCTG CTGCTGCTGG CAGTCCTGTG CCTGGGGACC 
    TCGGTGCGCT GCACCGAAGC CGGGAAGAGC AAGAGGCAGG CTCAGCAAAT CGTGCAGCCT
    CAATCCCCGG TGGCTGTCAG TCAGAGCAAG CCTGGCTGTT TTGACAATGG GAAGCACTAT
    CAGATAAATC AGCAGTGGGA ACGGACCTAC CTAGGCAACG CCCTGGTTTG TACCTGCTAT
    GGAGGAAGCC GGGGTTTTAA CTGCGAGAGC AAGCCTGAGC CTGAAGAGAC TTGCTTTGAC
    AAATACACTG GGAACACTTA CAAAGTGGGT GACACTTATG AGCGCCCTAA AGATTCCATG
    ATCTGGGACT GTACCTGCAT CGGGGCTGGG AGAGGCAGGA TCAGCTGTAC CATTGCAAAT
    CGCTGCCATG AAGGGGGTCA GTCCTACAAG ATTGGCGACA AGTGGAGGAG GCCACATGAG
    ACTGGTGGCT ACATGTTAGA GTGTCTGTGT CTGGGAAATG GAAAAGGGGA ATGGACCTGC
    AAACCTATAG CTGAGAAGTG TTTTGATCAT GCTGCTGGGA CGTCCTACGT CGTGGGGGAG
    ACCTGGGAAA AGCCCTACCA AGGCTGGATG ATGGTGGACT GTACTTGTCT AGGCGAAGGC
    AATGGACGCA TCACCTGTAC CTCCAGAAAC AGATGCAACG ATCAGGACAC CCGGACATCC
    TATAGGATTG GAGACACGTG GAGCAAGAAG GACAACCGAG GAAACCTGCT TCAGTGTGTC
    TGCACAGGCA ATGGCAGAGG GGAGTGGAAG TGTGAGCGAC ATGCTCTACA AAGTGCTTCA
    GCCGGATCTG GCTCCTTCAC TGATGTCCGA ACAGCTATTT ACCAACCGCA GACTCACCCC
    CAGCCCGCTC CCTACGGCCA CTGTGTCACC GACAGTGGTG TGGTCTACTC TGTGGGAATG
    CAGTGGCTGA AGTCGCAAGG AAACAAGCAA ATGCTGTGCA CGTGCCTGGG CAATGGCGTC
    AGCTGCCAGG AGACAGCCGT GACCCAGACT TATGGTGGCA ATTCAAACGG GGAGCCCTGT
    GTCCTCCCGT TCACCTACAA CGGTAGGACC TTCTATTCCT GCACCACCGA AGGGCGGCAA
    GACGGACATC TGTGGTGTAG CACAACTTCC AATTACGAAC AAGACCAGAA GTATTCCTTC
    TGCACAGACC ATGCGGTTTT GGTTCAGACT CGAGGCGGAA ATTCCAATGG TGCTCTGTGC
    CACTTCCCCT TCCTGTACAA CAACCGGAAT TACACCGACT GTACTTCTGA GGGTCGCAGG
    GACAACATGA AATGGTGCGG CACCACCCAG AACTACGATG CCGATCAGAA GTTTGGATTC
    TGCCCAATGG CTGCCCACGA GGAGATCTGC ACAACCAATG AAGGGGTCAT GTATCGCATT
    GGGGATCAGT GGGATAAGCA GCATGACCTG GGCCACATGA TGAGGTGCAC GTGTGTGGGG
    AACGGTCGTG GAGAATGGGC CTGCATCCCC TACTCCCAGC TCCGAGACCA GTGCATCGTT
    GATGACATTA CTTACAATGT GAACGACACG TTCCACAAGC GTCACGAGGA GGGACATATG
    CTGAACTGTA CCTGCTTTGG TCAGGGCCGG GGCAGATGGA AGTGTGACCC CATTGACCAG
    TGCCAAGATT CAGAGACCCG GACATTTTAC CAGATTGGTG ACTCCTGGGA GAAGTTTGTG
    CATGGTGTCC GATACCAGTG TTACTGCTAC GGCCGTGGCA TCGGGGAGTG GCACTGTCAA
    CCTCTGCAGA CCTACCCAGG CACAACTGGA CCTGTCCAAG TAATTATCAC GGAGACCCCC
    AGCCAGCCCA ATTCCCACCC CATCCAGTGG AATGCCCCGG AGCCTTCACA CATCACCAAG
    TACATTCTCA GATGGAGACC TAAAACCTCT ACGGGTCGCT GGAAGGAAGC TACCATTCCT
    GGCCACCTTA ACTCCTATAC CATCAAAGGC CTGACCCCAG GTGTGATCTA TGAGGGACAG
    CTCATCAGCA TCCAGCAGTA TGGCCACAGA GAAGTGACAC GCTTCGACTT CACCACCAGC
    GCCAGCACCC CTGTGACCAG CAACACGGTG ACCGGAGAGA CTGCGCCCTA CTCTCCTGTT
    GTGGCCACTT CTGAATCTGT AACTGAAATC ACAGCCAGCA GCTTTGTGGT CTCATGGGTC
    TCAGCCTCCG ACACCGTGTC AGGCTTCCGG GTGGAGTATG AGCTGAGCGA GGAGGGAGAT
    GAACCACAGT ACCTTGATCT CCCAAGCACG GCCACTTCCG TGAACATTCC TGACCTGCTC
    CCGGGCAGAA AGTACATTGT CAATGTCTAT CAGATATCTG AGGAGGGAAA ACAGAGCTTG
    ATCCTGTCTA CCTCACAGAC TACAGCACCT GACGCTCCTC CAGACCCTAC CGTGGACCAG
    GTTGATGATA CTTCCATTGT TGTTCGGTGG AGTAGACCCC AGGCACCTAT CACAGGGTAT
    AGAATTGTCT ATTCACCTTC AGTAGAAGGC AGTAGCACAG AGCTCAACCT CCCTGAAACG
    GCCAACTCCG TCACCCTCAG CGACCTGCAG CCCGGTGTTC AGTACAACAT CACTATCTAT
    GCTGTGGAGG AGAACCAGGA GAGCACACCC GTTTTCATCC AACAAGAGAC CACTGGCACC
    CCAAGATCTG ATAACGTCCC CCCTCCGACG GACCTACAGT TTGTGGAACT GACTGATGTG
    AAAGTCACCA TCATGTGGAC CCCTCCTGAT AGTGTGGTGT CTGGATACCG TGTGGAGGTC
    CTGCCTGTCA GCCTGCCCGG GGAACATGGG CAGAGGCTGC CTGTCAACAG AAATACCTTT
    GCTGAAATCA CTGGGCTGTC CCCTGGGGTC ACGTACCTCT TCAAAGTCTT TGCTGTGCAC
    CAGGGCAGGG AAAGCAATCC TCTGACGGCA CAACAGACCA CCAAACTCGA CGCTCCCACT
    AACCTCCAGT TTGTCAATGA AACTGACAGA ACAGTTCTGG TAACGTGGAC TCCACCTCGA
    GCCCGTATAG CAGGCTACCG ACTGACCGCG GGCCTGACCC GAGGAGGCCA GCCCAAGCAG
    TACAATGTGG GACCCTTGGC CTCCAAGTAT CCCCTGAGAA ATCTGCAGCC TGGGTCTGAG
    TACACCGTGA CCTTGGTGGC TGTGAAAGGG AACCAGCAGA GTCCCAAAGC CACCGGAGTC
    TTTACTACCC TGCAGCCTCT GCGCTCCATT CCACCTTACA ACACCGAGGT GACAGAGACC
    ACAATTGTGA TCACCTGGAC CCCCGCTCCA AGGATTGGCT TCAAGCTGGG TGTACGACCG
    AGCCAGGGAG GTGAGGCACC CCGAGAAGTG ACTTCAGACT CTGGGAGCAT TGTTGTGTCT
    GGCTTGACTC CAGGCGTGGA ATACACTTAC ACCATCCAAG TCCTGCGAGA TGGCCAGGAG
    AGAGATGCAC CGATTGTCAA CAGAGTAGTG ACACCGCTGT CTCCACCGAC CAACTTGCAT
    CTGGAGGCAA ACCCTGACAC TGGAGTGCTT ACTGTCTCCT GGGAGAGGAG CACTACCCCA
    GATATCACTG GCTACAGAAT AACTACTACC CCCACGAACG GGCAGCAGGG GACCTCTCTG
    GAAGAAGTGG TCCATGCTGA TCAGAGTTCC TGCACTTTTG AGAACCTGAA TCCTGGCCTG
    GAGTACAACG TCAGTGTTTA CACTGTCAAA GATGACAAGG AAAGTGCCCC TATCTCTGAT
    ACCGTTGTCC CAGAGGTGCC CCAGCTCACT GACCTAAGCT TTGTTGATAT AACTGATTCA
    AGCATCGGCC TGAGGTGGAC CCCGCTAAAC TCTTCCACCA TTATCGGGTA CCGAATCACA
    GTAGTTGCGG CAGGAGAAGG GATCCCTATT TTTGAAGATT TTGTGGACTC CTCAGTAGGA
    TACTACACAG TTACAGGGCT GGAGCCTGGC ATTGACTATG ACATCAGCGT TATCACTCTC
    ATTAATGGCG GAGAGAGTGC CCCTACTACA CTGACACAGC AAACGGCTGT CCCTCCTCCC
    ACGGATCTGC GATTCACCAA TATCGGTCCA GACACGATGC GGGTCACTTG GGCCCCGCCT
    CCGTCCATCG AGCTGACCAA CCTCTTGGTG CGCTACTCAC CCGTGAAGAA TGAAGAGGAC
    GTTGCAGAGC TATCCATTTC ACCTTCAGAC AATGCCGTGG TCCTAACAAA TCTCCTGCCT
    GGGACAGAAT ACTTAGTCAG TGTCTCCAGT GTCTACGAAC AACATGAGAG CATCCCTCTC
    CGGGGAAGGC AGAAAACAGG TCTCGATTCC CCAACTGGTT TTGATTCTTC TGATATCACC
    GCCAACTCAT TCACTGTCCA CTGGGTGGCT CCTCGGGCCC CCATCACCGG CTACATCATC
    CGCCATCACG CCGAGCATTC TGTCGGAAGA CCCAGGCAGG ATCGAGTGCC GCCCTCGCGG
    AATTCCATCA CCCTCACCAA CCTTAATCCG GGCACCGAGT ACGTTGTCAG CATCATTGCT
    GTTAATGGCA GAGAGGAGAG CCCGCCACTG ATTGGCCAGC AAGCCACAGT TTCTGATATT
    CCGAGAGATC TGGAGGTCAT TGCCTCCACC CCCACCAGCC TGCTCATCAG TTGGGAACCC
    CCTGCCGTCT CTGTGCGCTA TTACAGAATC ACCTACGGAG AGACAGGAGG AAATAGCCCT
    GTCCAGGAGT TCACTGTGCC CGGAAGCAAG TCCACAGCCA CCATCAACAA CATTAAACCA
    GGAGCAGACT ACACCATCAC CCTGTATGCT GTCACTGGCC GTGGGGACAG TCCAGCAAGC
    AGCAAGCCAG TTTCCATCAA TTATAAAACA GAAATTGACA AGCCGTCCCA GATGCAGGTG
    ACAGATGTCC AGGACAACAG CATCAGTGTC AGGTGGCTGC CTTCAACTTC TCCTGTGACA
    GGCTACAGAG TGACCACCAC TCCCAAAAAT GGCCTAGGAC CATCAAAAAC TAAAACTGCC
    AGTCCAGATC AAACAGAAAT GACCATTGAA GGTTTGCAAC CCACTGTGGA GTACGTGGTT
    AGTGTTTATG CTCAGAACCG GAACGGAGAA AGCCAGCCCC TGGTTCAAAC TGCAGTGACC
    AACATTGATC GCCCTAAAGG ACTGGCATTC ACTGATGTGG ATGTCGATTC CATCAAAATT
    GCTTGGGAAA GCCCACAGGG GCAAGTTTCC AGGTACAGGG TGACCTACTC GAGCCCTGAG
    GATGGAATCC GGGAGCTTTT CCCTGCACCT GATGGTGAAG ACGACACTGC AGAGCTGCAG
    GGCCTCAGGC CGGGGTCTGA GTACACAGTC AGTGTGGTTG CCTTGCACGA TGATATGGAG
    AGCCAGCCCC TGATTGGAAT CCAGTCCACA GCCATTCCTG CGCCCACCAA TCTGAAGTTC
    AGTCAGGTGA CACCCACCAG CTTTACTGCC CAGTGGATAG CACCCAGTGT TCAGCTCACT
    GGCTACCGGG TGCGGGTGAA CCCGAAAGAG AAGACAGGAC CAATGAAAGA AATCAACCTT
    TCTCCAGACA GCTCATCGGT GATTGTGTCA GGACTCATGG TGGCCACTAA ATACGAAGTC
    AGTGTCTATG CTCTCAAGGA CACACTGACA AGCAGACCAG CCCAGGGAGT CATCACTACT
    CTGGAGAATG TTAGCCCTCC AAGAAGGGCC CGTGTGACGG ACGCTACAGA GACCACCATC
    ACTATTAGCT GGAGAACAAA GACAGAGACA ATCACTGGCT TCCAAGTCGA TGCCATCCCA
    GCCAATGGCC AGACCCCAGT TCAGAGGAGC ATCAGCCCGG ATGTTAGAAG CTACACCATT
    ACAGGTTTAC AGCCAGGCAC TGACTACAAG ATCCACCTGT ACACTCTGAA CGACAATGCC
    CGGAGCTCGC CCGTGATCAT CGATGCCTCC ACCGCCATCG ACGCACCATC CAACCTGCGG
    TTCCTGACCA CCACACCCAA CTCCTTGCTG GTGTCATGGC AGGCGCCCCG TGCCAGGATT
    ACTGGCTACA TTATCAAGTA TGAGAAGCCT GGATCCCCTC CCAGAGAAGT GGTCCCTCGG
    CCCCGCCCTG GTGTCACGGA GGCCACCATT ACTGGTCTGG AGCCAGGAAC CGAGTACACC
    ATCTACGTCA TTGCCCTGAA GAACAATCAG AAGAGTGAGC CCCTGATTGG GAGGAAGAAG
    ACAGTTCAAA AGACCCCCTT CATCACCAAC CCTGGGTATG ACACCGAAAA TGGTATTCAG
    CTTCCTGGCA CAACCCACCA GCAACCCAGT GTTGGGCAAC AAATGATCTT TGAGGAACAT
    GGCTTTAGGC GGACAACGCC ACCCACTGCG GCCACCCCCG TCAGGCTTAG GCCAAGACCA
    TACCTGCCGA ATGTAGATGA GGAGGTCCAA ATCGGTCATG TTCCCAGGGG AGACGTAGAC
    TACCACCTCT ATCCTCATGT TCCGGGCCTC AATCCAAATG CCTCTACAGG ACAGGAAGCT
    CTCTCTCAGA CAACCATCTC TTGGACGCCG TTCCAGGAGA GTTCTGAGTA CATCATTTCA
    TGCCAACCAG TTGGCACCGA CGAAGAGCCC TTACAGTTCC AAGTTCCTGG AACTTCTACC
    AGTGCGACTC TGACTGGCCT TACCAGAGGG GTCACCTACA ACATCATAGT GGAGGCACTG
    CAGAACCAGA GGAGGCACAA GGTTCGGGAA GAGGTTGTGA CTGTGGGCAA CGCTGTCAGC
    GAAGGCCTGA ACCAGCCTAC AGATGACTCA TGCTTTGACC CTTACACGGT TTCCCATTAC
    GCCATTGGAG AGGAGTGGGA GCGGTTGTCT GACGCTGGCT TTAAGCTCAC ATGCCAGTGC
    TTGGGCTTTG GCAGTGGTCA TTTCAGATGC GATTCATCTA AATGGTGCCA TGACAACGGT
    GTCAACTACA AGATCGGAGA GAAGTGGGAT CGGCAGGGAG AAAATGGCCA GCGGATGAGC
    TGCACATGCC TCGGGAATGG AAAGGGAGAA TTCAAGTGTG ATCCCCATGA AGCAACGTGC
    TATGACGATG GGAAGACCTA CCATGTAGGA GAACAGTGGC AGAAAGAATA TCTCGGAGCC
    ATTTGCTCCT GCACGTGTTT CGGAGGCCAG CGGGGCTGGC GCTGTGACAA CTGCCGTAGA
    CCTGGGGCTG CTGAACCCAG TCCCGATGGC ACCACCGGCC ACACCTACAA CCAGTATACA
    CAGAGATACA ATCAGAGAAC AAACACTAAC GTAAATTGCC CCATTGAGTG CTTCATGCCG
    CTAGATGTGC AAGCTGACAG AGACGATTCT CGAGAGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu27267
    Clone ID Related Accession (Same CDS sequence) XM_006495699.3 , XM_006495699.4
    Accession Version XM_006495699.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7164bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product fibronectin isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006495699.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    ATGCTCAGGG GTCCGGGACC CGGGCGGCTG CTGCTGCTGG CAGTCCTGTG CCTGGGGACC 
    TCGGTGCGCT GCACCGAAGC CGGGAAGAGC AAGAGGCAGG CTCAGCAAAT CGTGCAGCCT
    CAATCCCCGG TGGCTGTCAG TCAGAGCAAG CCTGGCTGTT TTGACAATGG GAAGCACTAT
    CAGATAAATC AGCAGTGGGA ACGGACCTAC CTAGGCAACG CCCTGGTTTG TACCTGCTAT
    GGAGGAAGCC GGGGTTTTAA CTGCGAGAGC AAGCCTGAGC CTGAAGAGAC TTGCTTTGAC
    AAATACACTG GGAACACTTA CAAAGTGGGT GACACTTATG AGCGCCCTAA AGATTCCATG
    ATCTGGGACT GTACCTGCAT CGGGGCTGGG AGAGGCAGGA TCAGCTGTAC CATTGCAAAT
    CGCTGCCATG AAGGGGGTCA GTCCTACAAG ATTGGCGACA AGTGGAGGAG GCCACATGAG
    ACTGGTGGCT ACATGTTAGA GTGTCTGTGT CTGGGAAATG GAAAAGGGGA ATGGACCTGC
    AAACCTATAG CTGAGAAGTG TTTTGATCAT GCTGCTGGGA CGTCCTACGT CGTGGGGGAG
    ACCTGGGAAA AGCCCTACCA AGGCTGGATG ATGGTGGACT GTACTTGTCT AGGCGAAGGC
    AATGGACGCA TCACCTGTAC CTCCAGAAAC AGATGCAACG ATCAGGACAC CCGGACATCC
    TATAGGATTG GAGACACGTG GAGCAAGAAG GACAACCGAG GAAACCTGCT TCAGTGTGTC
    TGCACAGGCA ATGGCAGAGG GGAGTGGAAG TGTGAGCGAC ATGCTCTACA AAGTGCTTCA
    GCCGGATCTG GCTCCTTCAC TGATGTCCGA ACAGCTATTT ACCAACCGCA GACTCACCCC
    CAGCCCGCTC CCTACGGCCA CTGTGTCACC GACAGTGGTG TGGTCTACTC TGTGGGAATG
    CAGTGGCTGA AGTCGCAAGG AAACAAGCAA ATGCTGTGCA CGTGCCTGGG CAATGGCGTC
    AGCTGCCAGG AGACAGCCGT GACCCAGACT TATGGTGGCA ATTCAAACGG GGAGCCCTGT
    GTCCTCCCGT TCACCTACAA CGGTAGGACC TTCTATTCCT GCACCACCGA AGGGCGGCAA
    GACGGACATC TGTGGTGTAG CACAACTTCC AATTACGAAC AAGACCAGAA GTATTCCTTC
    TGCACAGACC ATGCGGTTTT GGTTCAGACT CGAGGCGGAA ATTCCAATGG TGCTCTGTGC
    CACTTCCCCT TCCTGTACAA CAACCGGAAT TACACCGACT GTACTTCTGA GGGTCGCAGG
    GACAACATGA AATGGTGCGG CACCACCCAG AACTACGATG CCGATCAGAA GTTTGGATTC
    TGCCCAATGG CTGCCCACGA GGAGATCTGC ACAACCAATG AAGGGGTCAT GTATCGCATT
    GGGGATCAGT GGGATAAGCA GCATGACCTG GGCCACATGA TGAGGTGCAC GTGTGTGGGG
    AACGGTCGTG GAGAATGGGC CTGCATCCCC TACTCCCAGC TCCGAGACCA GTGCATCGTT
    GATGACATTA CTTACAATGT GAACGACACG TTCCACAAGC GTCACGAGGA GGGACATATG
    CTGAACTGTA CCTGCTTTGG TCAGGGCCGG GGCAGATGGA AGTGTGACCC CATTGACCAG
    TGCCAAGATT CAGAGACCCG GACATTTTAC CAGATTGGTG ACTCCTGGGA GAAGTTTGTG
    CATGGTGTCC GATACCAGTG TTACTGCTAC GGCCGTGGCA TCGGGGAGTG GCACTGTCAA
    CCTCTGCAGA CCTACCCAGG CACAACTGGA CCTGTCCAAG TAATTATCAC GGAGACCCCC
    AGCCAGCCCA ATTCCCACCC CATCCAGTGG AATGCCCCGG AGCCTTCACA CATCACCAAG
    TACATTCTCA GATGGAGACC TAAAACCTCT ACGGGTCGCT GGAAGGAAGC TACCATTCCT
    GGCCACCTTA ACTCCTATAC CATCAAAGGC CTGACCCCAG GTGTGATCTA TGAGGGACAG
    CTCATCAGCA TCCAGCAGTA TGGCCACAGA GAAGTGACAC GCTTCGACTT CACCACCAGC
    GCCAGCACCC CTGTGACCAG CAACACGGTG ACCGGAGAGA CTGCGCCCTA CTCTCCTGTT
    GTGGCCACTT CTGAATCTGT AACTGAAATC ACAGCCAGCA GCTTTGTGGT CTCATGGGTC
    TCAGCCTCCG ACACCGTGTC AGGCTTCCGG GTGGAGTATG AGCTGAGCGA GGAGGGAGAT
    GAACCACAGT ACCTTGATCT CCCAAGCACG GCCACTTCCG TGAACATTCC TGACCTGCTC
    CCGGGCAGAA AGTACATTGT CAATGTCTAT CAGATATCTG AGGAGGGAAA ACAGAGCTTG
    ATCCTGTCTA CCTCACAGAC TACAGCACCT GACGCTCCTC CAGACCCTAC CGTGGACCAG
    GTTGATGATA CTTCCATTGT TGTTCGGTGG AGTAGACCCC AGGCACCTAT CACAGGGTAT
    AGAATTGTCT ATTCACCTTC AGTAGAAGGC AGTAGCACAG AGCTCAACCT CCCTGAAACG
    GCCAACTCCG TCACCCTCAG CGACCTGCAG CCCGGTGTTC AGTACAACAT CACTATCTAT
    GCTGTGGAGG AGAACCAGGA GAGCACACCC GTTTTCATCC AACAAGAGAC CACTGGCACC
    CCAAGATCTG ATAACGTCCC CCCTCCGACG GACCTACAGT TTGTGGAACT GACTGATGTG
    AAAGTCACCA TCATGTGGAC CCCTCCTGAT AGTGTGGTGT CTGGATACCG TGTGGAGGTC
    CTGCCTGTCA GCCTGCCCGG GGAACATGGG CAGAGGCTGC CTGTCAACAG AAATACCTTT
    GCTGAAATCA CTGGGCTGTC CCCTGGGGTC ACGTACCTCT TCAAAGTCTT TGCTGTGCAC
    CAGGGCAGGG AAAGCAATCC TCTGACGGCA CAACAGACCA CCAAACTCGA CGCTCCCACT
    AACCTCCAGT TTGTCAATGA AACTGACAGA ACAGTTCTGG TAACGTGGAC TCCACCTCGA
    GCCCGTATAG CAGGCTACCG ACTGACCGCG GGCCTGACCC GAGGAGGCCA GCCCAAGCAG
    TACAATGTGG GACCCTTGGC CTCCAAGTAT CCCCTGAGAA ATCTGCAGCC TGGGTCTGAG
    TACACCGTGA CCTTGGTGGC TGTGAAAGGG AACCAGCAGA GTCCCAAAGC CACCGGAGTC
    TTTACTACCC TGCAGCCTCT GCGCTCCATT CCACCTTACA ACACCGAGGT GACAGAGACC
    ACAATTGTGA TCACCTGGAC CCCCGCTCCA AGGATTGGCT TCAAGCTGGG TGTACGACCG
    AGCCAGGGAG GTGAGGCACC CCGAGAAGTG ACTTCAGACT CTGGGAGCAT TGTTGTGTCT
    GGCTTGACTC CAGGCGTGGA ATACACTTAC ACCATCCAAG TCCTGCGAGA TGGCCAGGAG
    AGAGATGCAC CGATTGTCAA CAGAGTAGTG ACACCGCTGT CTCCACCGAC CAACTTGCAT
    CTGGAGGCAA ACCCTGACAC TGGAGTGCTT ACTGTCTCCT GGGAGAGGAG CACTACCCCA
    GATATCACTG GCTACAGAAT AACTACTACC CCCACGAACG GGCAGCAGGG GACCTCTCTG
    GAAGAAGTGG TCCATGCTGA TCAGAGTTCC TGCACTTTTG AGAACCTGAA TCCTGGCCTG
    GAGTACAACG TCAGTGTTTA CACTGTCAAA GATGACAAGG AAAGTGCCCC TATCTCTGAT
    ACCGTTGTCC CAGAGGTGCC CCAGCTCACT GACCTAAGCT TTGTTGATAT AACTGATTCA
    AGCATCGGCC TGAGGTGGAC CCCGCTAAAC TCTTCCACCA TTATCGGGTA CCGAATCACA
    GTAGTTGCGG CAGGAGAAGG GATCCCTATT TTTGAAGATT TTGTGGACTC CTCAGTAGGA
    TACTACACAG TTACAGGGCT GGAGCCTGGC ATTGACTATG ACATCAGCGT TATCACTCTC
    ATTAATGGCG GAGAGAGTGC CCCTACTACA CTGACACAGC AAACGGCTGT CCCTCCTCCC
    ACGGATCTGC GATTCACCAA TATCGGTCCA GACACGATGC GGGTCACTTG GGCCCCGCCT
    CCGTCCATCG AGCTGACCAA CCTCTTGGTG CGCTACTCAC CCGTGAAGAA TGAAGAGGAC
    GTTGCAGAGC TATCCATTTC ACCTTCAGAC AATGCCGTGG TCCTAACAAA TCTCCTGCCT
    GGGACAGAAT ACTTAGTCAG TGTCTCCAGT GTCTACGAAC AACATGAGAG CATCCCTCTC
    CGGGGAAGGC AGAAAACAGG TCTCGATTCC CCAACTGGTT TTGATTCTTC TGATATCACC
    GCCAACTCAT TCACTGTCCA CTGGGTGGCT CCTCGGGCCC CCATCACCGG CTACATCATC
    CGCCATCACG CCGAGCATTC TGTCGGAAGA CCCAGGCAGG ATCGAGTGCC GCCCTCGCGG
    AATTCCATCA CCCTCACCAA CCTTAATCCG GGCACCGAGT ACGTTGTCAG CATCATTGCT
    GTTAATGGCA GAGAGGAGAG CCCGCCACTG ATTGGCCAGC AAGCCACAGT TTCTGATATT
    CCGAGAGATC TGGAGGTCAT TGCCTCCACC CCCACCAGCC TGCTCATCAG TTGGGAACCC
    CCTGCCGTCT CTGTGCGCTA TTACAGAATC ACCTACGGAG AGACAGGAGG AAATAGCCCT
    GTCCAGGAGT TCACTGTGCC CGGAAGCAAG TCCACAGCCA CCATCAACAA CATTAAACCA
    GGAGCAGACT ACACCATCAC CCTGTATGCT GTCACTGGCC GTGGGGACAG TCCAGCAAGC
    AGCAAGCCAG TTTCCATCAA TTATAAAACA GAAATTGACA AGCCGTCCCA GATGCAGGTG
    ACAGATGTCC AGGACAACAG CATCAGTGTC AGGTGGCTGC CTTCAACTTC TCCTGTGACA
    GGCTACAGAG TGACCACCAC TCCCAAAAAT GGCCTAGGAC CATCAAAAAC TAAAACTGCC
    AGTCCAGATC AAACAGAAAT GACCATTGAA GGTTTGCAAC CCACTGTGGA GTACGTGGTT
    AGTGTTTATG CTCAGAACCG GAACGGAGAA AGCCAGCCCC TGGTTCAAAC TGCAGTGACC
    ACCATTCCTG CGCCCACCAA TCTGAAGTTC AGTCAGGTGA CACCCACCAG CTTTACTGCC
    CAGTGGATAG CACCCAGTGT TCAGCTCACT GGCTACCGGG TGCGGGTGAA CCCGAAAGAG
    AAGACAGGAC CAATGAAAGA AATCAACCTT TCTCCAGACA GCTCATCGGT GATTGTGTCA
    GGACTCATGG TGGCCACTAA ATACGAAGTC AGTGTCTATG CTCTCAAGGA CACACTGACA
    AGCAGACCAG CCCAGGGAGT CATCACTACT CTGGAGAATG TTAGCCCTCC AAGAAGGGCC
    CGTGTGACGG ACGCTACAGA GACCACCATC ACTATTAGCT GGAGAACAAA GACAGAGACA
    ATCACTGGCT TCCAAGTCGA TGCCATCCCA GCCAATGGCC AGACCCCAGT TCAGAGGAGC
    ATCAGCCCGG ATGTTAGAAG CTACACCATT ACAGGTTTAC AGCCAGGCAC TGACTACAAG
    ATCCACCTGT ACACTCTGAA CGACAATGCC CGGAGCTCGC CCGTGATCAT CGATGCCTCC
    ACCGCCATCG ACGCACCATC CAACCTGCGG TTCCTGACCA CCACACCCAA CTCCTTGCTG
    GTGTCATGGC AGGCGCCCCG TGCCAGGATT ACTGGCTACA TTATCAAGTA TGAGAAGCCT
    GGATCCCCTC CCAGAGAAGT GGTCCCTCGG CCCCGCCCTG GTGTCACGGA GGCCACCATT
    ACTGGTCTGG AGCCAGGAAC CGAGTACACC ATCTACGTCA TTGCCCTGAA GAACAATCAG
    AAGAGTGAGC CCCTGATTGG GAGGAAGAAG ACAGATGAGC TTCCCCAACT GGTTACCCTT
    CCACACCCCA ATCTTCATGG ACCAGAGATC TTGGATGTTC CCTCCACAGT TCAAAAGACC
    CCCTTCATCA CCAACCCTGG GTATGACACC GAAAATGGTA TTCAGCTTCC TGGCACAACC
    CACCAGCAAC CCAGTGTTGG GCAACAAATG ATCTTTGAGG AACATGGCTT TAGGCGGACA
    ACGCCACCCA CTGCGGCCAC CCCCGTCAGG CTTAGGCCAA GACCATACCT GCCGAATGTA
    GATGAGGAGG TCCAAATCGG TCATGTTCCC AGGGGAGACG TAGACTACCA CCTCTATCCT
    CATGTTCCGG GCCTCAATCC AAATGCCTCT ACAGGACAGG AAGCTCTCTC TCAGACAACC
    ATCTCTTGGA CGCCGTTCCA GGAGAGTTCT GAGTACATCA TTTCATGCCA ACCAGTTGGC
    ACCGACGAAG AGCCCTTACA GTTCCAAGTT CCTGGAACTT CTACCAGTGC GACTCTGACT
    GGCCTTACCA GAGGGGTCAC CTACAACATC ATAGTGGAGG CACTGCAGAA CCAGAGGAGG
    CACAAGGTTC GGGAAGAGGT TGTGACTGTG GGCAACGCTG TCAGCGAAGG CCTGAACCAG
    CCTACAGATG ACTCATGCTT TGACCCTTAC ACGGTTTCCC ATTACGCCAT TGGAGAGGAG
    TGGGAGCGGT TGTCTGACGC TGGCTTTAAG CTCACATGCC AGTGCTTGGG CTTTGGCAGT
    GGTCATTTCA GATGCGATTC ATCTAAATGG TGCCATGACA ACGGTGTCAA CTACAAGATC
    GGAGAGAAGT GGGATCGGCA GGGAGAAAAT GGCCAGCGGA TGAGCTGCAC ATGCCTCGGG
    AATGGAAAGG GAGAATTCAA GTGTGATCCC CATGAAGCAA CGTGCTATGA CGATGGGAAG
    ACCTACCATG TAGGAGAACA GTGGCAGAAA GAATATCTCG GAGCCATTTG CTCCTGCACG
    TGTTTCGGAG GCCAGCGGGG CTGGCGCTGT GACAACTGCC GTAGACCTGG GGCTGCTGAA
    CCCAGTCCCG ATGGCACCAC CGGCCACACC TACAACCAGT ATACACAGAG ATACAATCAG
    AGAACAAACA CTAACGTAAA TTGCCCCATT GAGTGCTTCA TGCCGCTAGA TGTGCAAGCT
    GACAGAGACG ATTCTCGAGA GTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006495762.1
    CDS299..7462
    Translation

    Target ORF information:

    RefSeq Version XM_006495699.4
    Organism Mus musculus(house mouse)
    Definition Mus musculus fibronectin 1 (Fn1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006495699.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    ATGCTCAGGG GTCCGGGACC CGGGCGGCTG CTGCTGCTGG CAGTCCTGTG CCTGGGGACC 
    TCGGTGCGCT GCACCGAAGC CGGGAAGAGC AAGAGGCAGG CTCAGCAAAT CGTGCAGCCT
    CAATCCCCGG TGGCTGTCAG TCAGAGCAAG CCTGGCTGTT TTGACAATGG GAAGCACTAT
    CAGATAAATC AGCAGTGGGA ACGGACCTAC CTAGGCAACG CCCTGGTTTG TACCTGCTAT
    GGAGGAAGCC GGGGTTTTAA CTGCGAGAGC AAGCCTGAGC CTGAAGAGAC TTGCTTTGAC
    AAATACACTG GGAACACTTA CAAAGTGGGT GACACTTATG AGCGCCCTAA AGATTCCATG
    ATCTGGGACT GTACCTGCAT CGGGGCTGGG AGAGGCAGGA TCAGCTGTAC CATTGCAAAT
    CGCTGCCATG AAGGGGGTCA GTCCTACAAG ATTGGCGACA AGTGGAGGAG GCCACATGAG
    ACTGGTGGCT ACATGTTAGA GTGTCTGTGT CTGGGAAATG GAAAAGGGGA ATGGACCTGC
    AAACCTATAG CTGAGAAGTG TTTTGATCAT GCTGCTGGGA CGTCCTACGT CGTGGGGGAG
    ACCTGGGAAA AGCCCTACCA AGGCTGGATG ATGGTGGACT GTACTTGTCT AGGCGAAGGC
    AATGGACGCA TCACCTGTAC CTCCAGAAAC AGATGCAACG ATCAGGACAC CCGGACATCC
    TATAGGATTG GAGACACGTG GAGCAAGAAG GACAACCGAG GAAACCTGCT TCAGTGTGTC
    TGCACAGGCA ATGGCAGAGG GGAGTGGAAG TGTGAGCGAC ATGCTCTACA AAGTGCTTCA
    GCCGGATCTG GCTCCTTCAC TGATGTCCGA ACAGCTATTT ACCAACCGCA GACTCACCCC
    CAGCCCGCTC CCTACGGCCA CTGTGTCACC GACAGTGGTG TGGTCTACTC TGTGGGAATG
    CAGTGGCTGA AGTCGCAAGG AAACAAGCAA ATGCTGTGCA CGTGCCTGGG CAATGGCGTC
    AGCTGCCAGG AGACAGCCGT GACCCAGACT TATGGTGGCA ATTCAAACGG GGAGCCCTGT
    GTCCTCCCGT TCACCTACAA CGGTAGGACC TTCTATTCCT GCACCACCGA AGGGCGGCAA
    GACGGACATC TGTGGTGTAG CACAACTTCC AATTACGAAC AAGACCAGAA GTATTCCTTC
    TGCACAGACC ATGCGGTTTT GGTTCAGACT CGAGGCGGAA ATTCCAATGG TGCTCTGTGC
    CACTTCCCCT TCCTGTACAA CAACCGGAAT TACACCGACT GTACTTCTGA GGGTCGCAGG
    GACAACATGA AATGGTGCGG CACCACCCAG AACTACGATG CCGATCAGAA GTTTGGATTC
    TGCCCAATGG CTGCCCACGA GGAGATCTGC ACAACCAATG AAGGGGTCAT GTATCGCATT
    GGGGATCAGT GGGATAAGCA GCATGACCTG GGCCACATGA TGAGGTGCAC GTGTGTGGGG
    AACGGTCGTG GAGAATGGGC CTGCATCCCC TACTCCCAGC TCCGAGACCA GTGCATCGTT
    GATGACATTA CTTACAATGT GAACGACACG TTCCACAAGC GTCACGAGGA GGGACATATG
    CTGAACTGTA CCTGCTTTGG TCAGGGCCGG GGCAGATGGA AGTGTGACCC CATTGACCAG
    TGCCAAGATT CAGAGACCCG GACATTTTAC CAGATTGGTG ACTCCTGGGA GAAGTTTGTG
    CATGGTGTCC GATACCAGTG TTACTGCTAC GGCCGTGGCA TCGGGGAGTG GCACTGTCAA
    CCTCTGCAGA CCTACCCAGG CACAACTGGA CCTGTCCAAG TAATTATCAC GGAGACCCCC
    AGCCAGCCCA ATTCCCACCC CATCCAGTGG AATGCCCCGG AGCCTTCACA CATCACCAAG
    TACATTCTCA GATGGAGACC TAAAACCTCT ACGGGTCGCT GGAAGGAAGC TACCATTCCT
    GGCCACCTTA ACTCCTATAC CATCAAAGGC CTGACCCCAG GTGTGATCTA TGAGGGACAG
    CTCATCAGCA TCCAGCAGTA TGGCCACAGA GAAGTGACAC GCTTCGACTT CACCACCAGC
    GCCAGCACCC CTGTGACCAG CAACACGGTG ACCGGAGAGA CTGCGCCCTA CTCTCCTGTT
    GTGGCCACTT CTGAATCTGT AACTGAAATC ACAGCCAGCA GCTTTGTGGT CTCATGGGTC
    TCAGCCTCCG ACACCGTGTC AGGCTTCCGG GTGGAGTATG AGCTGAGCGA GGAGGGAGAT
    GAACCACAGT ACCTTGATCT CCCAAGCACG GCCACTTCCG TGAACATTCC TGACCTGCTC
    CCGGGCAGAA AGTACATTGT CAATGTCTAT CAGATATCTG AGGAGGGAAA ACAGAGCTTG
    ATCCTGTCTA CCTCACAGAC TACAGCACCT GACGCTCCTC CAGACCCTAC CGTGGACCAG
    GTTGATGATA CTTCCATTGT TGTTCGGTGG AGTAGACCCC AGGCACCTAT CACAGGGTAT
    AGAATTGTCT ATTCACCTTC AGTAGAAGGC AGTAGCACAG AGCTCAACCT CCCTGAAACG
    GCCAACTCCG TCACCCTCAG CGACCTGCAG CCCGGTGTTC AGTACAACAT CACTATCTAT
    GCTGTGGAGG AGAACCAGGA GAGCACACCC GTTTTCATCC AACAAGAGAC CACTGGCACC
    CCAAGATCTG ATAACGTCCC CCCTCCGACG GACCTACAGT TTGTGGAACT GACTGATGTG
    AAAGTCACCA TCATGTGGAC CCCTCCTGAT AGTGTGGTGT CTGGATACCG TGTGGAGGTC
    CTGCCTGTCA GCCTGCCCGG GGAACATGGG CAGAGGCTGC CTGTCAACAG AAATACCTTT
    GCTGAAATCA CTGGGCTGTC CCCTGGGGTC ACGTACCTCT TCAAAGTCTT TGCTGTGCAC
    CAGGGCAGGG AAAGCAATCC TCTGACGGCA CAACAGACCA CCAAACTCGA CGCTCCCACT
    AACCTCCAGT TTGTCAATGA AACTGACAGA ACAGTTCTGG TAACGTGGAC TCCACCTCGA
    GCCCGTATAG CAGGCTACCG ACTGACCGCG GGCCTGACCC GAGGAGGCCA GCCCAAGCAG
    TACAATGTGG GACCCTTGGC CTCCAAGTAT CCCCTGAGAA ATCTGCAGCC TGGGTCTGAG
    TACACCGTGA CCTTGGTGGC TGTGAAAGGG AACCAGCAGA GTCCCAAAGC CACCGGAGTC
    TTTACTACCC TGCAGCCTCT GCGCTCCATT CCACCTTACA ACACCGAGGT GACAGAGACC
    ACAATTGTGA TCACCTGGAC CCCCGCTCCA AGGATTGGCT TCAAGCTGGG TGTACGACCG
    AGCCAGGGAG GTGAGGCACC CCGAGAAGTG ACTTCAGACT CTGGGAGCAT TGTTGTGTCT
    GGCTTGACTC CAGGCGTGGA ATACACTTAC ACCATCCAAG TCCTGCGAGA TGGCCAGGAG
    AGAGATGCAC CGATTGTCAA CAGAGTAGTG ACACCGCTGT CTCCACCGAC CAACTTGCAT
    CTGGAGGCAA ACCCTGACAC TGGAGTGCTT ACTGTCTCCT GGGAGAGGAG CACTACCCCA
    GATATCACTG GCTACAGAAT AACTACTACC CCCACGAACG GGCAGCAGGG GACCTCTCTG
    GAAGAAGTGG TCCATGCTGA TCAGAGTTCC TGCACTTTTG AGAACCTGAA TCCTGGCCTG
    GAGTACAACG TCAGTGTTTA CACTGTCAAA GATGACAAGG AAAGTGCCCC TATCTCTGAT
    ACCGTTGTCC CAGAGGTGCC CCAGCTCACT GACCTAAGCT TTGTTGATAT AACTGATTCA
    AGCATCGGCC TGAGGTGGAC CCCGCTAAAC TCTTCCACCA TTATCGGGTA CCGAATCACA
    GTAGTTGCGG CAGGAGAAGG GATCCCTATT TTTGAAGATT TTGTGGACTC CTCAGTAGGA
    TACTACACAG TTACAGGGCT GGAGCCTGGC ATTGACTATG ACATCAGCGT TATCACTCTC
    ATTAATGGCG GAGAGAGTGC CCCTACTACA CTGACACAGC AAACGGCTGT CCCTCCTCCC
    ACGGATCTGC GATTCACCAA TATCGGTCCA GACACGATGC GGGTCACTTG GGCCCCGCCT
    CCGTCCATCG AGCTGACCAA CCTCTTGGTG CGCTACTCAC CCGTGAAGAA TGAAGAGGAC
    GTTGCAGAGC TATCCATTTC ACCTTCAGAC AATGCCGTGG TCCTAACAAA TCTCCTGCCT
    GGGACAGAAT ACTTAGTCAG TGTCTCCAGT GTCTACGAAC AACATGAGAG CATCCCTCTC
    CGGGGAAGGC AGAAAACAGG TCTCGATTCC CCAACTGGTT TTGATTCTTC TGATATCACC
    GCCAACTCAT TCACTGTCCA CTGGGTGGCT CCTCGGGCCC CCATCACCGG CTACATCATC
    CGCCATCACG CCGAGCATTC TGTCGGAAGA CCCAGGCAGG ATCGAGTGCC GCCCTCGCGG
    AATTCCATCA CCCTCACCAA CCTTAATCCG GGCACCGAGT ACGTTGTCAG CATCATTGCT
    GTTAATGGCA GAGAGGAGAG CCCGCCACTG ATTGGCCAGC AAGCCACAGT TTCTGATATT
    CCGAGAGATC TGGAGGTCAT TGCCTCCACC CCCACCAGCC TGCTCATCAG TTGGGAACCC
    CCTGCCGTCT CTGTGCGCTA TTACAGAATC ACCTACGGAG AGACAGGAGG AAATAGCCCT
    GTCCAGGAGT TCACTGTGCC CGGAAGCAAG TCCACAGCCA CCATCAACAA CATTAAACCA
    GGAGCAGACT ACACCATCAC CCTGTATGCT GTCACTGGCC GTGGGGACAG TCCAGCAAGC
    AGCAAGCCAG TTTCCATCAA TTATAAAACA GAAATTGACA AGCCGTCCCA GATGCAGGTG
    ACAGATGTCC AGGACAACAG CATCAGTGTC AGGTGGCTGC CTTCAACTTC TCCTGTGACA
    GGCTACAGAG TGACCACCAC TCCCAAAAAT GGCCTAGGAC CATCAAAAAC TAAAACTGCC
    AGTCCAGATC AAACAGAAAT GACCATTGAA GGTTTGCAAC CCACTGTGGA GTACGTGGTT
    AGTGTTTATG CTCAGAACCG GAACGGAGAA AGCCAGCCCC TGGTTCAAAC TGCAGTGACC
    ACCATTCCTG CGCCCACCAA TCTGAAGTTC AGTCAGGTGA CACCCACCAG CTTTACTGCC
    CAGTGGATAG CACCCAGTGT TCAGCTCACT GGCTACCGGG TGCGGGTGAA CCCGAAAGAG
    AAGACAGGAC CAATGAAAGA AATCAACCTT TCTCCAGACA GCTCATCGGT GATTGTGTCA
    GGACTCATGG TGGCCACTAA ATACGAAGTC AGTGTCTATG CTCTCAAGGA CACACTGACA
    AGCAGACCAG CCCAGGGAGT CATCACTACT CTGGAGAATG TTAGCCCTCC AAGAAGGGCC
    CGTGTGACGG ACGCTACAGA GACCACCATC ACTATTAGCT GGAGAACAAA GACAGAGACA
    ATCACTGGCT TCCAAGTCGA TGCCATCCCA GCCAATGGCC AGACCCCAGT TCAGAGGAGC
    ATCAGCCCGG ATGTTAGAAG CTACACCATT ACAGGTTTAC AGCCAGGCAC TGACTACAAG
    ATCCACCTGT ACACTCTGAA CGACAATGCC CGGAGCTCGC CCGTGATCAT CGATGCCTCC
    ACCGCCATCG ACGCACCATC CAACCTGCGG TTCCTGACCA CCACACCCAA CTCCTTGCTG
    GTGTCATGGC AGGCGCCCCG TGCCAGGATT ACTGGCTACA TTATCAAGTA TGAGAAGCCT
    GGATCCCCTC CCAGAGAAGT GGTCCCTCGG CCCCGCCCTG GTGTCACGGA GGCCACCATT
    ACTGGTCTGG AGCCAGGAAC CGAGTACACC ATCTACGTCA TTGCCCTGAA GAACAATCAG
    AAGAGTGAGC CCCTGATTGG GAGGAAGAAG ACAGATGAGC TTCCCCAACT GGTTACCCTT
    CCACACCCCA ATCTTCATGG ACCAGAGATC TTGGATGTTC CCTCCACAGT TCAAAAGACC
    CCCTTCATCA CCAACCCTGG GTATGACACC GAAAATGGTA TTCAGCTTCC TGGCACAACC
    CACCAGCAAC CCAGTGTTGG GCAACAAATG ATCTTTGAGG AACATGGCTT TAGGCGGACA
    ACGCCACCCA CTGCGGCCAC CCCCGTCAGG CTTAGGCCAA GACCATACCT GCCGAATGTA
    GATGAGGAGG TCCAAATCGG TCATGTTCCC AGGGGAGACG TAGACTACCA CCTCTATCCT
    CATGTTCCGG GCCTCAATCC AAATGCCTCT ACAGGACAGG AAGCTCTCTC TCAGACAACC
    ATCTCTTGGA CGCCGTTCCA GGAGAGTTCT GAGTACATCA TTTCATGCCA ACCAGTTGGC
    ACCGACGAAG AGCCCTTACA GTTCCAAGTT CCTGGAACTT CTACCAGTGC GACTCTGACT
    GGCCTTACCA GAGGGGTCAC CTACAACATC ATAGTGGAGG CACTGCAGAA CCAGAGGAGG
    CACAAGGTTC GGGAAGAGGT TGTGACTGTG GGCAACGCTG TCAGCGAAGG CCTGAACCAG
    CCTACAGATG ACTCATGCTT TGACCCTTAC ACGGTTTCCC ATTACGCCAT TGGAGAGGAG
    TGGGAGCGGT TGTCTGACGC TGGCTTTAAG CTCACATGCC AGTGCTTGGG CTTTGGCAGT
    GGTCATTTCA GATGCGATTC ATCTAAATGG TGCCATGACA ACGGTGTCAA CTACAAGATC
    GGAGAGAAGT GGGATCGGCA GGGAGAAAAT GGCCAGCGGA TGAGCTGCAC ATGCCTCGGG
    AATGGAAAGG GAGAATTCAA GTGTGATCCC CATGAAGCAA CGTGCTATGA CGATGGGAAG
    ACCTACCATG TAGGAGAACA GTGGCAGAAA GAATATCTCG GAGCCATTTG CTCCTGCACG
    TGTTTCGGAG GCCAGCGGGG CTGGCGCTGT GACAACTGCC GTAGACCTGG GGCTGCTGAA
    CCCAGTCCCG ATGGCACCAC CGGCCACACC TACAACCAGT ATACACAGAG ATACAATCAG
    AGAACAAACA CTAACGTAAA TTGCCCCATT GAGTGCTTCA TGCCGCTAGA TGTGCAAGCT
    GACAGAGACG ATTCTCGAGA GTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu27268
    Clone ID Related Accession (Same CDS sequence) XM_006495700.3 , XM_006495700.4
    Accession Version XM_006495700.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7074bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product fibronectin isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006495700.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    ATGCTCAGGG GTCCGGGACC CGGGCGGCTG CTGCTGCTGG CAGTCCTGTG CCTGGGGACC 
    TCGGTGCGCT GCACCGAAGC CGGGAAGAGC AAGAGGCAGG CTCAGCAAAT CGTGCAGCCT
    CAATCCCCGG TGGCTGTCAG TCAGAGCAAG CCTGGCTGTT TTGACAATGG GAAGCACTAT
    CAGATAAATC AGCAGTGGGA ACGGACCTAC CTAGGCAACG CCCTGGTTTG TACCTGCTAT
    GGAGGAAGCC GGGGTTTTAA CTGCGAGAGC AAGCCTGAGC CTGAAGAGAC TTGCTTTGAC
    AAATACACTG GGAACACTTA CAAAGTGGGT GACACTTATG AGCGCCCTAA AGATTCCATG
    ATCTGGGACT GTACCTGCAT CGGGGCTGGG AGAGGCAGGA TCAGCTGTAC CATTGCAAAT
    CGCTGCCATG AAGGGGGTCA GTCCTACAAG ATTGGCGACA AGTGGAGGAG GCCACATGAG
    ACTGGTGGCT ACATGTTAGA GTGTCTGTGT CTGGGAAATG GAAAAGGGGA ATGGACCTGC
    AAACCTATAG CTGAGAAGTG TTTTGATCAT GCTGCTGGGA CGTCCTACGT CGTGGGGGAG
    ACCTGGGAAA AGCCCTACCA AGGCTGGATG ATGGTGGACT GTACTTGTCT AGGCGAAGGC
    AATGGACGCA TCACCTGTAC CTCCAGAAAC AGATGCAACG ATCAGGACAC CCGGACATCC
    TATAGGATTG GAGACACGTG GAGCAAGAAG GACAACCGAG GAAACCTGCT TCAGTGTGTC
    TGCACAGGCA ATGGCAGAGG GGAGTGGAAG TGTGAGCGAC ATGCTCTACA AAGTGCTTCA
    GCCGGATCTG GCTCCTTCAC TGATGTCCGA ACAGCTATTT ACCAACCGCA GACTCACCCC
    CAGCCCGCTC CCTACGGCCA CTGTGTCACC GACAGTGGTG TGGTCTACTC TGTGGGAATG
    CAGTGGCTGA AGTCGCAAGG AAACAAGCAA ATGCTGTGCA CGTGCCTGGG CAATGGCGTC
    AGCTGCCAGG AGACAGCCGT GACCCAGACT TATGGTGGCA ATTCAAACGG GGAGCCCTGT
    GTCCTCCCGT TCACCTACAA CGGTAGGACC TTCTATTCCT GCACCACCGA AGGGCGGCAA
    GACGGACATC TGTGGTGTAG CACAACTTCC AATTACGAAC AAGACCAGAA GTATTCCTTC
    TGCACAGACC ATGCGGTTTT GGTTCAGACT CGAGGCGGAA ATTCCAATGG TGCTCTGTGC
    CACTTCCCCT TCCTGTACAA CAACCGGAAT TACACCGACT GTACTTCTGA GGGTCGCAGG
    GACAACATGA AATGGTGCGG CACCACCCAG AACTACGATG CCGATCAGAA GTTTGGATTC
    TGCCCAATGG CTGCCCACGA GGAGATCTGC ACAACCAATG AAGGGGTCAT GTATCGCATT
    GGGGATCAGT GGGATAAGCA GCATGACCTG GGCCACATGA TGAGGTGCAC GTGTGTGGGG
    AACGGTCGTG GAGAATGGGC CTGCATCCCC TACTCCCAGC TCCGAGACCA GTGCATCGTT
    GATGACATTA CTTACAATGT GAACGACACG TTCCACAAGC GTCACGAGGA GGGACATATG
    CTGAACTGTA CCTGCTTTGG TCAGGGCCGG GGCAGATGGA AGTGTGACCC CATTGACCAG
    TGCCAAGATT CAGAGACCCG GACATTTTAC CAGATTGGTG ACTCCTGGGA GAAGTTTGTG
    CATGGTGTCC GATACCAGTG TTACTGCTAC GGCCGTGGCA TCGGGGAGTG GCACTGTCAA
    CCTCTGCAGA CCTACCCAGG CACAACTGGA CCTGTCCAAG TAATTATCAC GGAGACCCCC
    AGCCAGCCCA ATTCCCACCC CATCCAGTGG AATGCCCCGG AGCCTTCACA CATCACCAAG
    TACATTCTCA GATGGAGACC TAAAACCTCT ACGGGTCGCT GGAAGGAAGC TACCATTCCT
    GGCCACCTTA ACTCCTATAC CATCAAAGGC CTGACCCCAG GTGTGATCTA TGAGGGACAG
    CTCATCAGCA TCCAGCAGTA TGGCCACAGA GAAGTGACAC GCTTCGACTT CACCACCAGC
    GCCAGCACCC CTGTGACCAG CAACACGGTG ACCGGAGAGA CTGCGCCCTA CTCTCCTGTT
    GTGGCCACTT CTGAATCTGT AACTGAAATC ACAGCCAGCA GCTTTGTGGT CTCATGGGTC
    TCAGCCTCCG ACACCGTGTC AGGCTTCCGG GTGGAGTATG AGCTGAGCGA GGAGGGAGAT
    GAACCACAGT ACCTTGATCT CCCAAGCACG GCCACTTCCG TGAACATTCC TGACCTGCTC
    CCGGGCAGAA AGTACATTGT CAATGTCTAT CAGATATCTG AGGAGGGAAA ACAGAGCTTG
    ATCCTGTCTA CCTCACAGAC TACAGCACCT GACGCTCCTC CAGACCCTAC CGTGGACCAG
    GTTGATGATA CTTCCATTGT TGTTCGGTGG AGTAGACCCC AGGCACCTAT CACAGGGTAT
    AGAATTGTCT ATTCACCTTC AGTAGAAGGC AGTAGCACAG AGCTCAACCT CCCTGAAACG
    GCCAACTCCG TCACCCTCAG CGACCTGCAG CCCGGTGTTC AGTACAACAT CACTATCTAT
    GCTGTGGAGG AGAACCAGGA GAGCACACCC GTTTTCATCC AACAAGAGAC CACTGGCACC
    CCAAGATCTG ATAACGTCCC CCCTCCGACG GACCTACAGT TTGTGGAACT GACTGATGTG
    AAAGTCACCA TCATGTGGAC CCCTCCTGAT AGTGTGGTGT CTGGATACCG TGTGGAGGTC
    CTGCCTGTCA GCCTGCCCGG GGAACATGGG CAGAGGCTGC CTGTCAACAG AAATACCTTT
    GCTGAAATCA CTGGGCTGTC CCCTGGGGTC ACGTACCTCT TCAAAGTCTT TGCTGTGCAC
    CAGGGCAGGG AAAGCAATCC TCTGACGGCA CAACAGACCA CCAAACTCGA CGCTCCCACT
    AACCTCCAGT TTGTCAATGA AACTGACAGA ACAGTTCTGG TAACGTGGAC TCCACCTCGA
    GCCCGTATAG CAGGCTACCG ACTGACCGCG GGCCTGACCC GAGGAGGCCA GCCCAAGCAG
    TACAATGTGG GACCCTTGGC CTCCAAGTAT CCCCTGAGAA ATCTGCAGCC TGGGTCTGAG
    TACACCGTGA CCTTGGTGGC TGTGAAAGGG AACCAGCAGA GTCCCAAAGC CACCGGAGTC
    TTTACTACCC TGCAGCCTCT GCGCTCCATT CCACCTTACA ACACCGAGGT GACAGAGACC
    ACAATTGTGA TCACCTGGAC CCCCGCTCCA AGGATTGGCT TCAAGCTGGG TGTACGACCG
    AGCCAGGGAG GTGAGGCACC CCGAGAAGTG ACTTCAGACT CTGGGAGCAT TGTTGTGTCT
    GGCTTGACTC CAGGCGTGGA ATACACTTAC ACCATCCAAG TCCTGCGAGA TGGCCAGGAG
    AGAGATGCAC CGATTGTCAA CAGAGTAGTG ACACCGCTGT CTCCACCGAC CAACTTGCAT
    CTGGAGGCAA ACCCTGACAC TGGAGTGCTT ACTGTCTCCT GGGAGAGGAG CACTACCCCA
    GATATCACTG GCTACAGAAT AACTACTACC CCCACGAACG GGCAGCAGGG GACCTCTCTG
    GAAGAAGTGG TCCATGCTGA TCAGAGTTCC TGCACTTTTG AGAACCTGAA TCCTGGCCTG
    GAGTACAACG TCAGTGTTTA CACTGTCAAA GATGACAAGG AAAGTGCCCC TATCTCTGAT
    ACCGTTGTCC CAGAGGTGCC CCAGCTCACT GACCTAAGCT TTGTTGATAT AACTGATTCA
    AGCATCGGCC TGAGGTGGAC CCCGCTAAAC TCTTCCACCA TTATCGGGTA CCGAATCACA
    GTAGTTGCGG CAGGAGAAGG GATCCCTATT TTTGAAGATT TTGTGGACTC CTCAGTAGGA
    TACTACACAG TTACAGGGCT GGAGCCTGGC ATTGACTATG ACATCAGCGT TATCACTCTC
    ATTAATGGCG GAGAGAGTGC CCCTACTACA CTGACACAGC AAACGGCTGT CCCTCCTCCC
    ACGGATCTGC GATTCACCAA TATCGGTCCA GACACGATGC GGGTCACTTG GGCCCCGCCT
    CCGTCCATCG AGCTGACCAA CCTCTTGGTG CGCTACTCAC CCGTGAAGAA TGAAGAGGAC
    GTTGCAGAGC TATCCATTTC ACCTTCAGAC AATGCCGTGG TCCTAACAAA TCTCCTGCCT
    GGGACAGAAT ACTTAGTCAG TGTCTCCAGT GTCTACGAAC AACATGAGAG CATCCCTCTC
    CGGGGAAGGC AGAAAACAGG TCTCGATTCC CCAACTGGTT TTGATTCTTC TGATATCACC
    GCCAACTCAT TCACTGTCCA CTGGGTGGCT CCTCGGGCCC CCATCACCGG CTACATCATC
    CGCCATCACG CCGAGCATTC TGTCGGAAGA CCCAGGCAGG ATCGAGTGCC GCCCTCGCGG
    AATTCCATCA CCCTCACCAA CCTTAATCCG GGCACCGAGT ACGTTGTCAG CATCATTGCT
    GTTAATGGCA GAGAGGAGAG CCCGCCACTG ATTGGCCAGC AAGCCACAGT TTCTGATATT
    CCGAGAGATC TGGAGGTCAT TGCCTCCACC CCCACCAGCC TGCTCATCAG TTGGGAACCC
    CCTGCCGTCT CTGTGCGCTA TTACAGAATC ACCTACGGAG AGACAGGAGG AAATAGCCCT
    GTCCAGGAGT TCACTGTGCC CGGAAGCAAG TCCACAGCCA CCATCAACAA CATTAAACCA
    GGAGCAGACT ACACCATCAC CCTGTATGCT GTCACTGGCC GTGGGGACAG TCCAGCAAGC
    AGCAAGCCAG TTTCCATCAA TTATAAAACA GAAATTGACA AGCCGTCCCA GATGCAGGTG
    ACAGATGTCC AGGACAACAG CATCAGTGTC AGGTGGCTGC CTTCAACTTC TCCTGTGACA
    GGCTACAGAG TGACCACCAC TCCCAAAAAT GGCCTAGGAC CATCAAAAAC TAAAACTGCC
    AGTCCAGATC AAACAGAAAT GACCATTGAA GGTTTGCAAC CCACTGTGGA GTACGTGGTT
    AGTGTTTATG CTCAGAACCG GAACGGAGAA AGCCAGCCCC TGGTTCAAAC TGCAGTGACC
    AACATTGATC GCCCTAAAGG ACTGGCATTC ACTGATGTGG ATGTCGATTC CATCAAAATT
    GCTTGGGAAA GCCCACAGGG GCAAGTTTCC AGGTACAGGG TGACCTACTC GAGCCCTGAG
    GATGGAATCC GGGAGCTTTT CCCTGCACCT GATGGTGAAG ACGACACTGC AGAGCTGCAG
    GGCCTCAGGC CGGGGTCTGA GTACACAGTC AGTGTGGTTG CCTTGCACGA TGATATGGAG
    AGCCAGCCCC TGATTGGAAT CCAGTCCACA GCCATTCCTG CGCCCACCAA TCTGAAGTTC
    AGTCAGGTGA CACCCACCAG CTTTACTGCC CAGTGGATAG CACCCAGTGT TCAGCTCACT
    GGCTACCGGG TGCGGGTGAA CCCGAAAGAG AAGACAGGAC CAATGAAAGA AATCAACCTT
    TCTCCAGACA GCTCATCGGT GATTGTGTCA GGACTCATGG TGGCCACTAA ATACGAAGTC
    AGTGTCTATG CTCTCAAGGA CACACTGACA AGCAGACCAG CCCAGGGAGT CATCACTACT
    CTGGAGAATG TTAGCCCTCC AAGAAGGGCC CGTGTGACGG ACGCTACAGA GACCACCATC
    ACTATTAGCT GGAGAACAAA GACAGAGACA ATCACTGGCT TCCAAGTCGA TGCCATCCCA
    GCCAATGGCC AGACCCCAGT TCAGAGGAGC ATCAGCCCGG ATGTTAGAAG CTACACCATT
    ACAGGTTTAC AGCCAGGCAC TGACTACAAG ATCCACCTGT ACACTCTGAA CGACAATGCC
    CGGAGCTCGC CCGTGATCAT CGATGCCTCC ACCGCCATCG ACGCACCATC CAACCTGCGG
    TTCCTGACCA CCACACCCAA CTCCTTGCTG GTGTCATGGC AGGCGCCCCG TGCCAGGATT
    ACTGGCTACA TTATCAAGTA TGAGAAGCCT GGATCCCCTC CCAGAGAAGT GGTCCCTCGG
    CCCCGCCCTG GTGTCACGGA GGCCACCATT ACTGGTCTGG AGCCAGGAAC CGAGTACACC
    ATCTACGTCA TTGCCCTGAA GAACAATCAG AAGAGTGAGC CCCTGATTGG GAGGAAGAAG
    ACAGGACAGG AAGCTCTCTC TCAGACAACC ATCTCTTGGA CGCCGTTCCA GGAGAGTTCT
    GAGTACATCA TTTCATGCCA ACCAGTTGGC ACCGACGAAG AGCCCTTACA GTTCCAAGTT
    CCTGGAACTT CTACCAGTGC GACTCTGACT GGCCTTACCA GAGGGGTCAC CTACAACATC
    ATAGTGGAGG CACTGCAGAA CCAGAGGAGG CACAAGGTTC GGGAAGAGGT TGTGACTGTG
    GGCAACGCTG TCAGCGAAGG CCTGAACCAG CCTACAGATG ACTCATGCTT TGACCCTTAC
    ACGGTTTCCC ATTACGCCAT TGGAGAGGAG TGGGAGCGGT TGTCTGACGC TGGCTTTAAG
    CTCACATGCC AGTGCTTGGG CTTTGGCAGT GGTCATTTCA GATGCGATTC ATCTAAATGG
    TGCCATGACA ACGGTGTCAA CTACAAGATC GGAGAGAAGT GGGATCGGCA GGGAGAAAAT
    GGCCAGCGGA TGAGCTGCAC ATGCCTCGGG AATGGAAAGG GAGAATTCAA GTGTGATCCC
    CATGAAGCAA CGTGCTATGA CGATGGGAAG ACCTACCATG TAGGAGAACA GTGGCAGAAA
    GAATATCTCG GAGCCATTTG CTCCTGCACG TGTTTCGGAG GCCAGCGGGG CTGGCGCTGT
    GACAACTGCC GTAGACCTGG GGCTGCTGAA CCCAGTCCCG ATGGCACCAC CGGCCACACC
    TACAACCAGT ATACACAGAG ATACAATCAG AGAACAAACA CTAACGTAAA TTGCCCCATT
    GAGTGCTTCA TGCCGCTAGA TGTGCAAGCT GACAGAGACG ATTCTCGAGA GTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006495763.1
    CDS299..7372
    Translation

    Target ORF information:

    RefSeq Version XM_006495700.4
    Organism Mus musculus(house mouse)
    Definition Mus musculus fibronectin 1 (Fn1), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006495700.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    ATGCTCAGGG GTCCGGGACC CGGGCGGCTG CTGCTGCTGG CAGTCCTGTG CCTGGGGACC 
    TCGGTGCGCT GCACCGAAGC CGGGAAGAGC AAGAGGCAGG CTCAGCAAAT CGTGCAGCCT
    CAATCCCCGG TGGCTGTCAG TCAGAGCAAG CCTGGCTGTT TTGACAATGG GAAGCACTAT
    CAGATAAATC AGCAGTGGGA ACGGACCTAC CTAGGCAACG CCCTGGTTTG TACCTGCTAT
    GGAGGAAGCC GGGGTTTTAA CTGCGAGAGC AAGCCTGAGC CTGAAGAGAC TTGCTTTGAC
    AAATACACTG GGAACACTTA CAAAGTGGGT GACACTTATG AGCGCCCTAA AGATTCCATG
    ATCTGGGACT GTACCTGCAT CGGGGCTGGG AGAGGCAGGA TCAGCTGTAC CATTGCAAAT
    CGCTGCCATG AAGGGGGTCA GTCCTACAAG ATTGGCGACA AGTGGAGGAG GCCACATGAG
    ACTGGTGGCT ACATGTTAGA GTGTCTGTGT CTGGGAAATG GAAAAGGGGA ATGGACCTGC
    AAACCTATAG CTGAGAAGTG TTTTGATCAT GCTGCTGGGA CGTCCTACGT CGTGGGGGAG
    ACCTGGGAAA AGCCCTACCA AGGCTGGATG ATGGTGGACT GTACTTGTCT AGGCGAAGGC
    AATGGACGCA TCACCTGTAC CTCCAGAAAC AGATGCAACG ATCAGGACAC CCGGACATCC
    TATAGGATTG GAGACACGTG GAGCAAGAAG GACAACCGAG GAAACCTGCT TCAGTGTGTC
    TGCACAGGCA ATGGCAGAGG GGAGTGGAAG TGTGAGCGAC ATGCTCTACA AAGTGCTTCA
    GCCGGATCTG GCTCCTTCAC TGATGTCCGA ACAGCTATTT ACCAACCGCA GACTCACCCC
    CAGCCCGCTC CCTACGGCCA CTGTGTCACC GACAGTGGTG TGGTCTACTC TGTGGGAATG
    CAGTGGCTGA AGTCGCAAGG AAACAAGCAA ATGCTGTGCA CGTGCCTGGG CAATGGCGTC
    AGCTGCCAGG AGACAGCCGT GACCCAGACT TATGGTGGCA ATTCAAACGG GGAGCCCTGT
    GTCCTCCCGT TCACCTACAA CGGTAGGACC TTCTATTCCT GCACCACCGA AGGGCGGCAA
    GACGGACATC TGTGGTGTAG CACAACTTCC AATTACGAAC AAGACCAGAA GTATTCCTTC
    TGCACAGACC ATGCGGTTTT GGTTCAGACT CGAGGCGGAA ATTCCAATGG TGCTCTGTGC
    CACTTCCCCT TCCTGTACAA CAACCGGAAT TACACCGACT GTACTTCTGA GGGTCGCAGG
    GACAACATGA AATGGTGCGG CACCACCCAG AACTACGATG CCGATCAGAA GTTTGGATTC
    TGCCCAATGG CTGCCCACGA GGAGATCTGC ACAACCAATG AAGGGGTCAT GTATCGCATT
    GGGGATCAGT GGGATAAGCA GCATGACCTG GGCCACATGA TGAGGTGCAC GTGTGTGGGG
    AACGGTCGTG GAGAATGGGC CTGCATCCCC TACTCCCAGC TCCGAGACCA GTGCATCGTT
    GATGACATTA CTTACAATGT GAACGACACG TTCCACAAGC GTCACGAGGA GGGACATATG
    CTGAACTGTA CCTGCTTTGG TCAGGGCCGG GGCAGATGGA AGTGTGACCC CATTGACCAG
    TGCCAAGATT CAGAGACCCG GACATTTTAC CAGATTGGTG ACTCCTGGGA GAAGTTTGTG
    CATGGTGTCC GATACCAGTG TTACTGCTAC GGCCGTGGCA TCGGGGAGTG GCACTGTCAA
    CCTCTGCAGA CCTACCCAGG CACAACTGGA CCTGTCCAAG TAATTATCAC GGAGACCCCC
    AGCCAGCCCA ATTCCCACCC CATCCAGTGG AATGCCCCGG AGCCTTCACA CATCACCAAG
    TACATTCTCA GATGGAGACC TAAAACCTCT ACGGGTCGCT GGAAGGAAGC TACCATTCCT
    GGCCACCTTA ACTCCTATAC CATCAAAGGC CTGACCCCAG GTGTGATCTA TGAGGGACAG
    CTCATCAGCA TCCAGCAGTA TGGCCACAGA GAAGTGACAC GCTTCGACTT CACCACCAGC
    GCCAGCACCC CTGTGACCAG CAACACGGTG ACCGGAGAGA CTGCGCCCTA CTCTCCTGTT
    GTGGCCACTT CTGAATCTGT AACTGAAATC ACAGCCAGCA GCTTTGTGGT CTCATGGGTC
    TCAGCCTCCG ACACCGTGTC AGGCTTCCGG GTGGAGTATG AGCTGAGCGA GGAGGGAGAT
    GAACCACAGT ACCTTGATCT CCCAAGCACG GCCACTTCCG TGAACATTCC TGACCTGCTC
    CCGGGCAGAA AGTACATTGT CAATGTCTAT CAGATATCTG AGGAGGGAAA ACAGAGCTTG
    ATCCTGTCTA CCTCACAGAC TACAGCACCT GACGCTCCTC CAGACCCTAC CGTGGACCAG
    GTTGATGATA CTTCCATTGT TGTTCGGTGG AGTAGACCCC AGGCACCTAT CACAGGGTAT
    AGAATTGTCT ATTCACCTTC AGTAGAAGGC AGTAGCACAG AGCTCAACCT CCCTGAAACG
    GCCAACTCCG TCACCCTCAG CGACCTGCAG CCCGGTGTTC AGTACAACAT CACTATCTAT
    GCTGTGGAGG AGAACCAGGA GAGCACACCC GTTTTCATCC AACAAGAGAC CACTGGCACC
    CCAAGATCTG ATAACGTCCC CCCTCCGACG GACCTACAGT TTGTGGAACT GACTGATGTG
    AAAGTCACCA TCATGTGGAC CCCTCCTGAT AGTGTGGTGT CTGGATACCG TGTGGAGGTC
    CTGCCTGTCA GCCTGCCCGG GGAACATGGG CAGAGGCTGC CTGTCAACAG AAATACCTTT
    GCTGAAATCA CTGGGCTGTC CCCTGGGGTC ACGTACCTCT TCAAAGTCTT TGCTGTGCAC
    CAGGGCAGGG AAAGCAATCC TCTGACGGCA CAACAGACCA CCAAACTCGA CGCTCCCACT
    AACCTCCAGT TTGTCAATGA AACTGACAGA ACAGTTCTGG TAACGTGGAC TCCACCTCGA
    GCCCGTATAG CAGGCTACCG ACTGACCGCG GGCCTGACCC GAGGAGGCCA GCCCAAGCAG
    TACAATGTGG GACCCTTGGC CTCCAAGTAT CCCCTGAGAA ATCTGCAGCC TGGGTCTGAG
    TACACCGTGA CCTTGGTGGC TGTGAAAGGG AACCAGCAGA GTCCCAAAGC CACCGGAGTC
    TTTACTACCC TGCAGCCTCT GCGCTCCATT CCACCTTACA ACACCGAGGT GACAGAGACC
    ACAATTGTGA TCACCTGGAC CCCCGCTCCA AGGATTGGCT TCAAGCTGGG TGTACGACCG
    AGCCAGGGAG GTGAGGCACC CCGAGAAGTG ACTTCAGACT CTGGGAGCAT TGTTGTGTCT
    GGCTTGACTC CAGGCGTGGA ATACACTTAC ACCATCCAAG TCCTGCGAGA TGGCCAGGAG
    AGAGATGCAC CGATTGTCAA CAGAGTAGTG ACACCGCTGT CTCCACCGAC CAACTTGCAT
    CTGGAGGCAA ACCCTGACAC TGGAGTGCTT ACTGTCTCCT GGGAGAGGAG CACTACCCCA
    GATATCACTG GCTACAGAAT AACTACTACC CCCACGAACG GGCAGCAGGG GACCTCTCTG
    GAAGAAGTGG TCCATGCTGA TCAGAGTTCC TGCACTTTTG AGAACCTGAA TCCTGGCCTG
    GAGTACAACG TCAGTGTTTA CACTGTCAAA GATGACAAGG AAAGTGCCCC TATCTCTGAT
    ACCGTTGTCC CAGAGGTGCC CCAGCTCACT GACCTAAGCT TTGTTGATAT AACTGATTCA
    AGCATCGGCC TGAGGTGGAC CCCGCTAAAC TCTTCCACCA TTATCGGGTA CCGAATCACA
    GTAGTTGCGG CAGGAGAAGG GATCCCTATT TTTGAAGATT TTGTGGACTC CTCAGTAGGA
    TACTACACAG TTACAGGGCT GGAGCCTGGC ATTGACTATG ACATCAGCGT TATCACTCTC
    ATTAATGGCG GAGAGAGTGC CCCTACTACA CTGACACAGC AAACGGCTGT CCCTCCTCCC
    ACGGATCTGC GATTCACCAA TATCGGTCCA GACACGATGC GGGTCACTTG GGCCCCGCCT
    CCGTCCATCG AGCTGACCAA CCTCTTGGTG CGCTACTCAC CCGTGAAGAA TGAAGAGGAC
    GTTGCAGAGC TATCCATTTC ACCTTCAGAC AATGCCGTGG TCCTAACAAA TCTCCTGCCT
    GGGACAGAAT ACTTAGTCAG TGTCTCCAGT GTCTACGAAC AACATGAGAG CATCCCTCTC
    CGGGGAAGGC AGAAAACAGG TCTCGATTCC CCAACTGGTT TTGATTCTTC TGATATCACC
    GCCAACTCAT TCACTGTCCA CTGGGTGGCT CCTCGGGCCC CCATCACCGG CTACATCATC
    CGCCATCACG CCGAGCATTC TGTCGGAAGA CCCAGGCAGG ATCGAGTGCC GCCCTCGCGG
    AATTCCATCA CCCTCACCAA CCTTAATCCG GGCACCGAGT ACGTTGTCAG CATCATTGCT
    GTTAATGGCA GAGAGGAGAG CCCGCCACTG ATTGGCCAGC AAGCCACAGT TTCTGATATT
    CCGAGAGATC TGGAGGTCAT TGCCTCCACC CCCACCAGCC TGCTCATCAG TTGGGAACCC
    CCTGCCGTCT CTGTGCGCTA TTACAGAATC ACCTACGGAG AGACAGGAGG AAATAGCCCT
    GTCCAGGAGT TCACTGTGCC CGGAAGCAAG TCCACAGCCA CCATCAACAA CATTAAACCA
    GGAGCAGACT ACACCATCAC CCTGTATGCT GTCACTGGCC GTGGGGACAG TCCAGCAAGC
    AGCAAGCCAG TTTCCATCAA TTATAAAACA GAAATTGACA AGCCGTCCCA GATGCAGGTG
    ACAGATGTCC AGGACAACAG CATCAGTGTC AGGTGGCTGC CTTCAACTTC TCCTGTGACA
    GGCTACAGAG TGACCACCAC TCCCAAAAAT GGCCTAGGAC CATCAAAAAC TAAAACTGCC
    AGTCCAGATC AAACAGAAAT GACCATTGAA GGTTTGCAAC CCACTGTGGA GTACGTGGTT
    AGTGTTTATG CTCAGAACCG GAACGGAGAA AGCCAGCCCC TGGTTCAAAC TGCAGTGACC
    AACATTGATC GCCCTAAAGG ACTGGCATTC ACTGATGTGG ATGTCGATTC CATCAAAATT
    GCTTGGGAAA GCCCACAGGG GCAAGTTTCC AGGTACAGGG TGACCTACTC GAGCCCTGAG
    GATGGAATCC GGGAGCTTTT CCCTGCACCT GATGGTGAAG ACGACACTGC AGAGCTGCAG
    GGCCTCAGGC CGGGGTCTGA GTACACAGTC AGTGTGGTTG CCTTGCACGA TGATATGGAG
    AGCCAGCCCC TGATTGGAAT CCAGTCCACA GCCATTCCTG CGCCCACCAA TCTGAAGTTC
    AGTCAGGTGA CACCCACCAG CTTTACTGCC CAGTGGATAG CACCCAGTGT TCAGCTCACT
    GGCTACCGGG TGCGGGTGAA CCCGAAAGAG AAGACAGGAC CAATGAAAGA AATCAACCTT
    TCTCCAGACA GCTCATCGGT GATTGTGTCA GGACTCATGG TGGCCACTAA ATACGAAGTC
    AGTGTCTATG CTCTCAAGGA CACACTGACA AGCAGACCAG CCCAGGGAGT CATCACTACT
    CTGGAGAATG TTAGCCCTCC AAGAAGGGCC CGTGTGACGG ACGCTACAGA GACCACCATC
    ACTATTAGCT GGAGAACAAA GACAGAGACA ATCACTGGCT TCCAAGTCGA TGCCATCCCA
    GCCAATGGCC AGACCCCAGT TCAGAGGAGC ATCAGCCCGG ATGTTAGAAG CTACACCATT
    ACAGGTTTAC AGCCAGGCAC TGACTACAAG ATCCACCTGT ACACTCTGAA CGACAATGCC
    CGGAGCTCGC CCGTGATCAT CGATGCCTCC ACCGCCATCG ACGCACCATC CAACCTGCGG
    TTCCTGACCA CCACACCCAA CTCCTTGCTG GTGTCATGGC AGGCGCCCCG TGCCAGGATT
    ACTGGCTACA TTATCAAGTA TGAGAAGCCT GGATCCCCTC CCAGAGAAGT GGTCCCTCGG
    CCCCGCCCTG GTGTCACGGA GGCCACCATT ACTGGTCTGG AGCCAGGAAC CGAGTACACC
    ATCTACGTCA TTGCCCTGAA GAACAATCAG AAGAGTGAGC CCCTGATTGG GAGGAAGAAG
    ACAGGACAGG AAGCTCTCTC TCAGACAACC ATCTCTTGGA CGCCGTTCCA GGAGAGTTCT
    GAGTACATCA TTTCATGCCA ACCAGTTGGC ACCGACGAAG AGCCCTTACA GTTCCAAGTT
    CCTGGAACTT CTACCAGTGC GACTCTGACT GGCCTTACCA GAGGGGTCAC CTACAACATC
    ATAGTGGAGG CACTGCAGAA CCAGAGGAGG CACAAGGTTC GGGAAGAGGT TGTGACTGTG
    GGCAACGCTG TCAGCGAAGG CCTGAACCAG CCTACAGATG ACTCATGCTT TGACCCTTAC
    ACGGTTTCCC ATTACGCCAT TGGAGAGGAG TGGGAGCGGT TGTCTGACGC TGGCTTTAAG
    CTCACATGCC AGTGCTTGGG CTTTGGCAGT GGTCATTTCA GATGCGATTC ATCTAAATGG
    TGCCATGACA ACGGTGTCAA CTACAAGATC GGAGAGAAGT GGGATCGGCA GGGAGAAAAT
    GGCCAGCGGA TGAGCTGCAC ATGCCTCGGG AATGGAAAGG GAGAATTCAA GTGTGATCCC
    CATGAAGCAA CGTGCTATGA CGATGGGAAG ACCTACCATG TAGGAGAACA GTGGCAGAAA
    GAATATCTCG GAGCCATTTG CTCCTGCACG TGTTTCGGAG GCCAGCGGGG CTGGCGCTGT
    GACAACTGCC GTAGACCTGG GGCTGCTGAA CCCAGTCCCG ATGGCACCAC CGGCCACACC
    TACAACCAGT ATACACAGAG ATACAATCAG AGAACAAACA CTAACGTAAA TTGCCCCATT
    GAGTGCTTCA TGCCGCTAGA TGTGCAAGCT GACAGAGACG ATTCTCGAGA GTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu76976
    Clone ID Related Accession (Same CDS sequence) XM_017315775.2 , XM_017315775.1
    Accession Version XM_017315775.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7269bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product fibronectin isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_017315775.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    ATGCTCAGGG GTCCGGGACC CGGGCGGCTG CTGCTGCTGG CAGTCCTGTG CCTGGGGACC 
    TCGGTGCGCT GCACCGAAGC CGGGAAGAGC AAGAGGCAGG CTCAGCAAAT CGTGCAGCCT
    CAATCCCCGG TGGCTGTCAG TCAGAGCAAG CCTGGCTGTT TTGACAATGG GAAGCACTAT
    CAGATAAATC AGCAGTGGGA ACGGACCTAC CTAGGCAACG CCCTGGTTTG TACCTGCTAT
    GGAGGAAGCC GGGGTTTTAA CTGCGAGAGC AAGCCTGAGC CTGAAGAGAC TTGCTTTGAC
    AAATACACTG GGAACACTTA CAAAGTGGGT GACACTTATG AGCGCCCTAA AGATTCCATG
    ATCTGGGACT GTACCTGCAT CGGGGCTGGG AGAGGCAGGA TCAGCTGTAC CATTGCAAAT
    CGCTGCCATG AAGGGGGTCA GTCCTACAAG ATTGGCGACA AGTGGAGGAG GCCACATGAG
    ACTGGTGGCT ACATGTTAGA GTGTCTGTGT CTGGGAAATG GAAAAGGGGA ATGGACCTGC
    AAACCTATAG CTGAGAAGTG TTTTGATCAT GCTGCTGGGA CGTCCTACGT CGTGGGGGAG
    ACCTGGGAAA AGCCCTACCA AGGCTGGATG ATGGTGGACT GTACTTGTCT AGGCGAAGGC
    AATGGACGCA TCACCTGTAC CTCCAGAAAC AGATGCAACG ATCAGGACAC CCGGACATCC
    TATAGGATTG GAGACACGTG GAGCAAGAAG GACAACCGAG GAAACCTGCT TCAGTGTGTC
    TGCACAGGCA ATGGCAGAGG GGAGTGGAAG TGTGAGCGAC ATGCTCTACA AAGTGCTTCA
    GCCGGATCTG GCTCCTTCAC TGATGTCCGA ACAGCTATTT ACCAACCGCA GACTCACCCC
    CAGCCCGCTC CCTACGGCCA CTGTGTCACC GACAGTGGTG TGGTCTACTC TGTGGGAATG
    CAGTGGCTGA AGTCGCAAGG AAACAAGCAA ATGCTGTGCA CGTGCCTGGG CAATGGCGTC
    AGCTGCCAGG AGACAGCCGT GACCCAGACT TATGGTGGCA ATTCAAACGG GGAGCCCTGT
    GTCCTCCCGT TCACCTACAA CGGTAGGACC TTCTATTCCT GCACCACCGA AGGGCGGCAA
    GACGGACATC TGTGGTGTAG CACAACTTCC AATTACGAAC AAGACCAGAA GTATTCCTTC
    TGCACAGACC ATGCGGTTTT GGTTCAGACT CGAGGCGGAA ATTCCAATGG TGCTCTGTGC
    CACTTCCCCT TCCTGTACAA CAACCGGAAT TACACCGACT GTACTTCTGA GGGTCGCAGG
    GACAACATGA AATGGTGCGG CACCACCCAG AACTACGATG CCGATCAGAA GTTTGGATTC
    TGCCCAATGG CTGCCCACGA GGAGATCTGC ACAACCAATG AAGGGGTCAT GTATCGCATT
    GGGGATCAGT GGGATAAGCA GCATGACCTG GGCCACATGA TGAGGTGCAC GTGTGTGGGG
    AACGGTCGTG GAGAATGGGC CTGCATCCCC TACTCCCAGC TCCGAGACCA GTGCATCGTT
    GATGACATTA CTTACAATGT GAACGACACG TTCCACAAGC GTCACGAGGA GGGACATATG
    CTGAACTGTA CCTGCTTTGG TCAGGGCCGG GGCAGATGGA AGTGTGACCC CATTGACCAG
    TGCCAAGATT CAGAGACCCG GACATTTTAC CAGATTGGTG ACTCCTGGGA GAAGTTTGTG
    CATGGTGTCC GATACCAGTG TTACTGCTAC GGCCGTGGCA TCGGGGAGTG GCACTGTCAA
    CCTCTGCAGA CCTACCCAGG CACAACTGGA CCTGTCCAAG TAATTATCAC GGAGACCCCC
    AGCCAGCCCA ATTCCCACCC CATCCAGTGG AATGCCCCGG AGCCTTCACA CATCACCAAG
    TACATTCTCA GATGGAGACC TAAAACCTCT ACGGGTCGCT GGAAGGAAGC TACCATTCCT
    GGCCACCTTA ACTCCTATAC CATCAAAGGC CTGACCCCAG GTGTGATCTA TGAGGGACAG
    CTCATCAGCA TCCAGCAGTA TGGCCACAGA GAAGTGACAC GCTTCGACTT CACCACCAGC
    GCCAGCACCC CTGTGACCAG CAACACGGTG ACCGGAGAGA CTGCGCCCTA CTCTCCTGTT
    GTGGCCACTT CTGAATCTGT AACTGAAATC ACAGCCAGCA GCTTTGTGGT CTCATGGGTC
    TCAGCCTCCG ACACCGTGTC AGGCTTCCGG GTGGAGTATG AGCTGAGCGA GGAGGGAGAT
    GAACCACAGT ACCTTGATCT CCCAAGCACG GCCACTTCCG TGAACATTCC TGACCTGCTC
    CCGGGCAGAA AGTACATTGT CAATGTCTAT CAGATATCTG AGGAGGGAAA ACAGAGCTTG
    ATCCTGTCTA CCTCACAGAC TACAGCACCT GACGCTCCTC CAGACCCTAC CGTGGACCAG
    GTTGATGATA CTTCCATTGT TGTTCGGTGG AGTAGACCCC AGGCACCTAT CACAGGGTAT
    AGAATTGTCT ATTCACCTTC AGTAGAAGGC AGTAGCACAG AGCTCAACCT CCCTGAAACG
    GCCAACTCCG TCACCCTCAG CGACCTGCAG CCCGGTGTTC AGTACAACAT CACTATCTAT
    GCTGTGGAGG AGAACCAGGA GAGCACACCC GTTTTCATCC AACAAGAGAC CACTGGCACC
    CCAAGATCTG ATAACGTCCC CCCTCCGACG GACCTACAGT TTGTGGAACT GACTGATGTG
    AAAGTCACCA TCATGTGGAC CCCTCCTGAT AGTGTGGTGT CTGGATACCG TGTGGAGGTC
    CTGCCTGTCA GCCTGCCCGG GGAACATGGG CAGAGGCTGC CTGTCAACAG AAATACCTTT
    GCTGAAATCA CTGGGCTGTC CCCTGGGGTC ACGTACCTCT TCAAAGTCTT TGCTGTGCAC
    CAGGGCAGGG AAAGCAATCC TCTGACGGCA CAACAGACCA CCAAACTCGA CGCTCCCACT
    AACCTCCAGT TTGTCAATGA AACTGACAGA ACAGTTCTGG TAACGTGGAC TCCACCTCGA
    GCCCGTATAG CAGGCTACCG ACTGACCGCG GGCCTGACCC GAGGAGGCCA GCCCAAGCAG
    TACAATGTGG GACCCTTGGC CTCCAAGTAT CCCCTGAGAA ATCTGCAGCC TGGGTCTGAG
    TACACCGTGA CCTTGGTGGC TGTGAAAGGG AACCAGCAGA GTCCCAAAGC CACCGGAGTC
    TTTACTACCC TGCAGCCTCT GCGCTCCATT CCACCTTACA ACACCGAGGT GACAGAGACC
    ACAATTGTGA TCACCTGGAC CCCCGCTCCA AGGATTGGCT TCAAGCTGGG TGTACGACCG
    AGCCAGGGAG GTGAGGCACC CCGAGAAGTG ACTTCAGACT CTGGGAGCAT TGTTGTGTCT
    GGCTTGACTC CAGGCGTGGA ATACACTTAC ACCATCCAAG TCCTGCGAGA TGGCCAGGAG
    AGAGATGCAC CGATTGTCAA CAGAGTAGTG ACACCGCTGT CTCCACCGAC CAACTTGCAT
    CTGGAGGCAA ACCCTGACAC TGGAGTGCTT ACTGTCTCCT GGGAGAGGAG CACTACCCCA
    GATATCACTG GCTACAGAAT AACTACTACC CCCACGAACG GGCAGCAGGG GACCTCTCTG
    GAAGAAGTGG TCCATGCTGA TCAGAGTTCC TGCACTTTTG AGAACCTGAA TCCTGGCCTG
    GAGTACAACG TCAGTGTTTA CACTGTCAAA GATGACAAGG AAAGTGCCCC TATCTCTGAT
    ACCGTTGTCC CAGAGGTGCC CCAGCTCACT GACCTAAGCT TTGTTGATAT AACTGATTCA
    AGCATCGGCC TGAGGTGGAC CCCGCTAAAC TCTTCCACCA TTATCGGGTA CCGAATCACA
    GTAGTTGCGG CAGGAGAAGG GATCCCTATT TTTGAAGATT TTGTGGACTC CTCAGTAGGA
    TACTACACAG TTACAGGGCT GGAGCCTGGC ATTGACTATG ACATCAGCGT TATCACTCTC
    ATTAATGGCG GAGAGAGTGC CCCTACTACA CTGACACAGC AAACGGCTGT CCCTCCTCCC
    ACGGATCTGC GATTCACCAA TATCGGTCCA GACACGATGC GGGTCACTTG GGCCCCGCCT
    CCGTCCATCG AGCTGACCAA CCTCTTGGTG CGCTACTCAC CCGTGAAGAA TGAAGAGGAC
    GTTGCAGAGC TATCCATTTC ACCTTCAGAC AATGCCGTGG TCCTAACAAA TCTCCTGCCT
    GGGACAGAAT ACTTAGTCAG TGTCTCCAGT GTCTACGAAC AACATGAGAG CATCCCTCTC
    CGGGGAAGGC AGAAAACAGG TCTCGATTCC CCAACTGGTT TTGATTCTTC TGATATCACC
    GCCAACTCAT TCACTGTCCA CTGGGTGGCT CCTCGGGCCC CCATCACCGG CTACATCATC
    CGCCATCACG CCGAGCATTC TGTCGGAAGA CCCAGGCAGG ATCGAGTGCC GCCCTCGCGG
    AATTCCATCA CCCTCACCAA CCTTAATCCG GGCACCGAGT ACGTTGTCAG CATCATTGCT
    GTTAATGGCA GAGAGGAGAG CCCGCCACTG ATTGGCCAGC AAGCCACAGT TTCTGATATT
    CCGAGAGATC TGGAGGTCAT TGCCTCCACC CCCACCAGCC TGCTCATCAG TTGGGAACCC
    CCTGCCGTCT CTGTGCGCTA TTACAGAATC ACCTACGGAG AGACAGGAGG AAATAGCCCT
    GTCCAGGAGT TCACTGTGCC CGGAAGCAAG TCCACAGCCA CCATCAACAA CATTAAACCA
    GGAGCAGACT ACACCATCAC CCTGTATGCT GTCACTGGCC GTGGGGACAG TCCAGCAAGC
    AGCAAGCCAG TTTCCATCAA TTATAAAACA GAAATTGACA AGCCGTCCCA GATGCAGGTG
    ACAGATGTCC AGGACAACAG CATCAGTGTC AGGTGGCTGC CTTCAACTTC TCCTGTGACA
    GGCTACAGAG TGACCACCAC TCCCAAAAAT GGCCTAGGAC CATCAAAAAC TAAAACTGCC
    AGTCCAGATC AAACAGAAAT GACCATTGAA GGTTTGCAAC CCACTGTGGA GTACGTGGTT
    AGTGTTTATG CTCAGAACCG GAACGGAGAA AGCCAGCCCC TGGTTCAAAC TGCAGTGACC
    AACATTGATC GCCCTAAAGG ACTGGCATTC ACTGATGTGG ATGTCGATTC CATCAAAATT
    GCTTGGGAAA GCCCACAGGG GCAAGTTTCC AGGTACAGGG TGACCTACTC GAGCCCTGAG
    GATGGAATCC GGGAGCTTTT CCCTGCACCT GATGGTGAAG ACGACACTGC AGAGCTGCAG
    GGCCTCAGGC CGGGGTCTGA GTACACAGTC AGTGTGGTTG CCTTGCACGA TGATATGGAG
    AGCCAGCCCC TGATTGGAAT CCAGTCCACA GCCATTCCTG CGCCCACCAA TCTGAAGTTC
    AGTCAGGTGA CACCCACCAG CTTTACTGCC CAGTGGATAG CACCCAGTGT TCAGCTCACT
    GGCTACCGGG TGCGGGTGAA CCCGAAAGAG AAGACAGGAC CAATGAAAGA AATCAACCTT
    TCTCCAGACA GCTCATCGGT GATTGTGTCA GGACTCATGG TGGCCACTAA ATACGAAGTC
    AGTGTCTATG CTCTCAAGGA CACACTGACA AGCAGACCAG CCCAGGGAGT CATCACTACT
    CTGGAGAATG TTAGCCCTCC AAGAAGGGCC CGTGTGACGG ACGCTACAGA GACCACCATC
    ACTATTAGCT GGAGAACAAA GACAGAGACA ATCACTGGCT TCCAAGTCGA TGCCATCCCA
    GCCAATGGCC AGACCCCAGT TCAGAGGAGC ATCAGCCCGG ATGTTAGAAG CTACACCATT
    ACAGGTTTAC AGCCAGGCAC TGACTACAAG ATCCACCTGT ACACTCTGAA CGACAATGCC
    CGGAGCTCGC CCGTGATCAT CGATGCCTCC ACCGCCATCG ACGCACCATC CAACCTGCGG
    TTCCTGACCA CCACACCCAA CTCCTTGCTG GTGTCATGGC AGGCGCCCCG TGCCAGGATT
    ACTGGCTACA TTATCAAGTA TGAGAAGCCT GGATCCCCTC CCAGAGAAGT GGTCCCTCGG
    CCCCGCCCTG GTGTCACGGA GGCCACCATT ACTGGTCTGG AGCCAGGAAC CGAGTACACC
    ATCTACGTCA TTGCCCTGAA GAACAATCAG AAGAGTGAGC CCCTGATTGG GAGGAAGAAG
    ACAGATGAGC TTCCCCAACT GGTTACCCTT CCACACCCCA ATCTTCATGG ACCAGAGATC
    TTGGATGTTC CCTCCACAGT TCAAAAGACC CCCTTCATCA CCAACCCTGG GTATGACACC
    GAAAATGGTA TTCAGCTTCC TGGCACAACC CACCAGCAAC CCAGTGTTGG GCAACAAATG
    ATCTTTGAGG AACATGGCTT TAGGCGGACA ACGCCACCCA CTGCGGCCAC CCCCGTCAGG
    CTTAGGCCAA GACCATACCT GCCGAATGTA GATGAGGAGG TCCAAATCGG TCATGTTCCC
    AGGGGAGACG TAGACTACCA CCTCTATCCT CATGTTCCGG GCCTCAATCC AAATGCCTCT
    ACAGGACAGG AAGCTCTCTC TCAGACAACC ATCTCTTGGA CGCCGTTCCA GGAGAGTTCT
    GAGTACATCA TTTCATGCCA ACCAGTTGGC ACCGACGAAG AGCCCTTACA GTTCCAAGTT
    CCTGGAACTT CTACCAGTGC GACTCTGACT GGCCTTACCA GAGGGGTCAC CTACAACATC
    ATAGTGGAGG CACTGCAGAA CCAGAGGAGG CACAAGGTTC GGGAAGAGGT TGTGACTGTG
    GGCAACGCTG AATGGTGCCA TGACAACGGT GTCAACTACA AGATCGGAGA GAAGTGGGAT
    CGGCAGGGAG AAAATGGCCA GCGGATGAGC TGCACATGCC TCGGGAATGG AAAGGGAGAA
    TTCAAGTGTG ATCCCCATGA AGCAACGTGC TATGACGATG GGAAGACCTA CCATGTAGGA
    GAACAGTGGC AGAAAGAATA TCTCGGAGCC ATTTGCTCCT GCACGTGTTT CGGAGGCCAG
    CGGGGCTGGC GCTGTGACAA CTGCCGTAGA CCTGGGGCTG CTGAACCCAG TCCCGATGGC
    ACCACCGGCC ACACCTACAA CCAGTATACA CAGAGATACA ATCAGAGAAC AAACACTAAC
    GTAAATTGCC CCATTGAGTG CTTCATGCCG CTAGATGTGC AAGCTGACAG AGACGATTCT
    CGAGAGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017171264.1
    CDS299..7567
    Translation

    Target ORF information:

    RefSeq Version XM_017315775.2
    Organism Mus musculus(house mouse)
    Definition Mus musculus fibronectin 1 (Fn1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017315775.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    ATGCTCAGGG GTCCGGGACC CGGGCGGCTG CTGCTGCTGG CAGTCCTGTG CCTGGGGACC 
    TCGGTGCGCT GCACCGAAGC CGGGAAGAGC AAGAGGCAGG CTCAGCAAAT CGTGCAGCCT
    CAATCCCCGG TGGCTGTCAG TCAGAGCAAG CCTGGCTGTT TTGACAATGG GAAGCACTAT
    CAGATAAATC AGCAGTGGGA ACGGACCTAC CTAGGCAACG CCCTGGTTTG TACCTGCTAT
    GGAGGAAGCC GGGGTTTTAA CTGCGAGAGC AAGCCTGAGC CTGAAGAGAC TTGCTTTGAC
    AAATACACTG GGAACACTTA CAAAGTGGGT GACACTTATG AGCGCCCTAA AGATTCCATG
    ATCTGGGACT GTACCTGCAT CGGGGCTGGG AGAGGCAGGA TCAGCTGTAC CATTGCAAAT
    CGCTGCCATG AAGGGGGTCA GTCCTACAAG ATTGGCGACA AGTGGAGGAG GCCACATGAG
    ACTGGTGGCT ACATGTTAGA GTGTCTGTGT CTGGGAAATG GAAAAGGGGA ATGGACCTGC
    AAACCTATAG CTGAGAAGTG TTTTGATCAT GCTGCTGGGA CGTCCTACGT CGTGGGGGAG
    ACCTGGGAAA AGCCCTACCA AGGCTGGATG ATGGTGGACT GTACTTGTCT AGGCGAAGGC
    AATGGACGCA TCACCTGTAC CTCCAGAAAC AGATGCAACG ATCAGGACAC CCGGACATCC
    TATAGGATTG GAGACACGTG GAGCAAGAAG GACAACCGAG GAAACCTGCT TCAGTGTGTC
    TGCACAGGCA ATGGCAGAGG GGAGTGGAAG TGTGAGCGAC ATGCTCTACA AAGTGCTTCA
    GCCGGATCTG GCTCCTTCAC TGATGTCCGA ACAGCTATTT ACCAACCGCA GACTCACCCC
    CAGCCCGCTC CCTACGGCCA CTGTGTCACC GACAGTGGTG TGGTCTACTC TGTGGGAATG
    CAGTGGCTGA AGTCGCAAGG AAACAAGCAA ATGCTGTGCA CGTGCCTGGG CAATGGCGTC
    AGCTGCCAGG AGACAGCCGT GACCCAGACT TATGGTGGCA ATTCAAACGG GGAGCCCTGT
    GTCCTCCCGT TCACCTACAA CGGTAGGACC TTCTATTCCT GCACCACCGA AGGGCGGCAA
    GACGGACATC TGTGGTGTAG CACAACTTCC AATTACGAAC AAGACCAGAA GTATTCCTTC
    TGCACAGACC ATGCGGTTTT GGTTCAGACT CGAGGCGGAA ATTCCAATGG TGCTCTGTGC
    CACTTCCCCT TCCTGTACAA CAACCGGAAT TACACCGACT GTACTTCTGA GGGTCGCAGG
    GACAACATGA AATGGTGCGG CACCACCCAG AACTACGATG CCGATCAGAA GTTTGGATTC
    TGCCCAATGG CTGCCCACGA GGAGATCTGC ACAACCAATG AAGGGGTCAT GTATCGCATT
    GGGGATCAGT GGGATAAGCA GCATGACCTG GGCCACATGA TGAGGTGCAC GTGTGTGGGG
    AACGGTCGTG GAGAATGGGC CTGCATCCCC TACTCCCAGC TCCGAGACCA GTGCATCGTT
    GATGACATTA CTTACAATGT GAACGACACG TTCCACAAGC GTCACGAGGA GGGACATATG
    CTGAACTGTA CCTGCTTTGG TCAGGGCCGG GGCAGATGGA AGTGTGACCC CATTGACCAG
    TGCCAAGATT CAGAGACCCG GACATTTTAC CAGATTGGTG ACTCCTGGGA GAAGTTTGTG
    CATGGTGTCC GATACCAGTG TTACTGCTAC GGCCGTGGCA TCGGGGAGTG GCACTGTCAA
    CCTCTGCAGA CCTACCCAGG CACAACTGGA CCTGTCCAAG TAATTATCAC GGAGACCCCC
    AGCCAGCCCA ATTCCCACCC CATCCAGTGG AATGCCCCGG AGCCTTCACA CATCACCAAG
    TACATTCTCA GATGGAGACC TAAAACCTCT ACGGGTCGCT GGAAGGAAGC TACCATTCCT
    GGCCACCTTA ACTCCTATAC CATCAAAGGC CTGACCCCAG GTGTGATCTA TGAGGGACAG
    CTCATCAGCA TCCAGCAGTA TGGCCACAGA GAAGTGACAC GCTTCGACTT CACCACCAGC
    GCCAGCACCC CTGTGACCAG CAACACGGTG ACCGGAGAGA CTGCGCCCTA CTCTCCTGTT
    GTGGCCACTT CTGAATCTGT AACTGAAATC ACAGCCAGCA GCTTTGTGGT CTCATGGGTC
    TCAGCCTCCG ACACCGTGTC AGGCTTCCGG GTGGAGTATG AGCTGAGCGA GGAGGGAGAT
    GAACCACAGT ACCTTGATCT CCCAAGCACG GCCACTTCCG TGAACATTCC TGACCTGCTC
    CCGGGCAGAA AGTACATTGT CAATGTCTAT CAGATATCTG AGGAGGGAAA ACAGAGCTTG
    ATCCTGTCTA CCTCACAGAC TACAGCACCT GACGCTCCTC CAGACCCTAC CGTGGACCAG
    GTTGATGATA CTTCCATTGT TGTTCGGTGG AGTAGACCCC AGGCACCTAT CACAGGGTAT
    AGAATTGTCT ATTCACCTTC AGTAGAAGGC AGTAGCACAG AGCTCAACCT CCCTGAAACG
    GCCAACTCCG TCACCCTCAG CGACCTGCAG CCCGGTGTTC AGTACAACAT CACTATCTAT
    GCTGTGGAGG AGAACCAGGA GAGCACACCC GTTTTCATCC AACAAGAGAC CACTGGCACC
    CCAAGATCTG ATAACGTCCC CCCTCCGACG GACCTACAGT TTGTGGAACT GACTGATGTG
    AAAGTCACCA TCATGTGGAC CCCTCCTGAT AGTGTGGTGT CTGGATACCG TGTGGAGGTC
    CTGCCTGTCA GCCTGCCCGG GGAACATGGG CAGAGGCTGC CTGTCAACAG AAATACCTTT
    GCTGAAATCA CTGGGCTGTC CCCTGGGGTC ACGTACCTCT TCAAAGTCTT TGCTGTGCAC
    CAGGGCAGGG AAAGCAATCC TCTGACGGCA CAACAGACCA CCAAACTCGA CGCTCCCACT
    AACCTCCAGT TTGTCAATGA AACTGACAGA ACAGTTCTGG TAACGTGGAC TCCACCTCGA
    GCCCGTATAG CAGGCTACCG ACTGACCGCG GGCCTGACCC GAGGAGGCCA GCCCAAGCAG
    TACAATGTGG GACCCTTGGC CTCCAAGTAT CCCCTGAGAA ATCTGCAGCC TGGGTCTGAG
    TACACCGTGA CCTTGGTGGC TGTGAAAGGG AACCAGCAGA GTCCCAAAGC CACCGGAGTC
    TTTACTACCC TGCAGCCTCT GCGCTCCATT CCACCTTACA ACACCGAGGT GACAGAGACC
    ACAATTGTGA TCACCTGGAC CCCCGCTCCA AGGATTGGCT TCAAGCTGGG TGTACGACCG
    AGCCAGGGAG GTGAGGCACC CCGAGAAGTG ACTTCAGACT CTGGGAGCAT TGTTGTGTCT
    GGCTTGACTC CAGGCGTGGA ATACACTTAC ACCATCCAAG TCCTGCGAGA TGGCCAGGAG
    AGAGATGCAC CGATTGTCAA CAGAGTAGTG ACACCGCTGT CTCCACCGAC CAACTTGCAT
    CTGGAGGCAA ACCCTGACAC TGGAGTGCTT ACTGTCTCCT GGGAGAGGAG CACTACCCCA
    GATATCACTG GCTACAGAAT AACTACTACC CCCACGAACG GGCAGCAGGG GACCTCTCTG
    GAAGAAGTGG TCCATGCTGA TCAGAGTTCC TGCACTTTTG AGAACCTGAA TCCTGGCCTG
    GAGTACAACG TCAGTGTTTA CACTGTCAAA GATGACAAGG AAAGTGCCCC TATCTCTGAT
    ACCGTTGTCC CAGAGGTGCC CCAGCTCACT GACCTAAGCT TTGTTGATAT AACTGATTCA
    AGCATCGGCC TGAGGTGGAC CCCGCTAAAC TCTTCCACCA TTATCGGGTA CCGAATCACA
    GTAGTTGCGG CAGGAGAAGG GATCCCTATT TTTGAAGATT TTGTGGACTC CTCAGTAGGA
    TACTACACAG TTACAGGGCT GGAGCCTGGC ATTGACTATG ACATCAGCGT TATCACTCTC
    ATTAATGGCG GAGAGAGTGC CCCTACTACA CTGACACAGC AAACGGCTGT CCCTCCTCCC
    ACGGATCTGC GATTCACCAA TATCGGTCCA GACACGATGC GGGTCACTTG GGCCCCGCCT
    CCGTCCATCG AGCTGACCAA CCTCTTGGTG CGCTACTCAC CCGTGAAGAA TGAAGAGGAC
    GTTGCAGAGC TATCCATTTC ACCTTCAGAC AATGCCGTGG TCCTAACAAA TCTCCTGCCT
    GGGACAGAAT ACTTAGTCAG TGTCTCCAGT GTCTACGAAC AACATGAGAG CATCCCTCTC
    CGGGGAAGGC AGAAAACAGG TCTCGATTCC CCAACTGGTT TTGATTCTTC TGATATCACC
    GCCAACTCAT TCACTGTCCA CTGGGTGGCT CCTCGGGCCC CCATCACCGG CTACATCATC
    CGCCATCACG CCGAGCATTC TGTCGGAAGA CCCAGGCAGG ATCGAGTGCC GCCCTCGCGG
    AATTCCATCA CCCTCACCAA CCTTAATCCG GGCACCGAGT ACGTTGTCAG CATCATTGCT
    GTTAATGGCA GAGAGGAGAG CCCGCCACTG ATTGGCCAGC AAGCCACAGT TTCTGATATT
    CCGAGAGATC TGGAGGTCAT TGCCTCCACC CCCACCAGCC TGCTCATCAG TTGGGAACCC
    CCTGCCGTCT CTGTGCGCTA TTACAGAATC ACCTACGGAG AGACAGGAGG AAATAGCCCT
    GTCCAGGAGT TCACTGTGCC CGGAAGCAAG TCCACAGCCA CCATCAACAA CATTAAACCA
    GGAGCAGACT ACACCATCAC CCTGTATGCT GTCACTGGCC GTGGGGACAG TCCAGCAAGC
    AGCAAGCCAG TTTCCATCAA TTATAAAACA GAAATTGACA AGCCGTCCCA GATGCAGGTG
    ACAGATGTCC AGGACAACAG CATCAGTGTC AGGTGGCTGC CTTCAACTTC TCCTGTGACA
    GGCTACAGAG TGACCACCAC TCCCAAAAAT GGCCTAGGAC CATCAAAAAC TAAAACTGCC
    AGTCCAGATC AAACAGAAAT GACCATTGAA GGTTTGCAAC CCACTGTGGA GTACGTGGTT
    AGTGTTTATG CTCAGAACCG GAACGGAGAA AGCCAGCCCC TGGTTCAAAC TGCAGTGACC
    AACATTGATC GCCCTAAAGG ACTGGCATTC ACTGATGTGG ATGTCGATTC CATCAAAATT
    GCTTGGGAAA GCCCACAGGG GCAAGTTTCC AGGTACAGGG TGACCTACTC GAGCCCTGAG
    GATGGAATCC GGGAGCTTTT CCCTGCACCT GATGGTGAAG ACGACACTGC AGAGCTGCAG
    GGCCTCAGGC CGGGGTCTGA GTACACAGTC AGTGTGGTTG CCTTGCACGA TGATATGGAG
    AGCCAGCCCC TGATTGGAAT CCAGTCCACA GCCATTCCTG CGCCCACCAA TCTGAAGTTC
    AGTCAGGTGA CACCCACCAG CTTTACTGCC CAGTGGATAG CACCCAGTGT TCAGCTCACT
    GGCTACCGGG TGCGGGTGAA CCCGAAAGAG AAGACAGGAC CAATGAAAGA AATCAACCTT
    TCTCCAGACA GCTCATCGGT GATTGTGTCA GGACTCATGG TGGCCACTAA ATACGAAGTC
    AGTGTCTATG CTCTCAAGGA CACACTGACA AGCAGACCAG CCCAGGGAGT CATCACTACT
    CTGGAGAATG TTAGCCCTCC AAGAAGGGCC CGTGTGACGG ACGCTACAGA GACCACCATC
    ACTATTAGCT GGAGAACAAA GACAGAGACA ATCACTGGCT TCCAAGTCGA TGCCATCCCA
    GCCAATGGCC AGACCCCAGT TCAGAGGAGC ATCAGCCCGG ATGTTAGAAG CTACACCATT
    ACAGGTTTAC AGCCAGGCAC TGACTACAAG ATCCACCTGT ACACTCTGAA CGACAATGCC
    CGGAGCTCGC CCGTGATCAT CGATGCCTCC ACCGCCATCG ACGCACCATC CAACCTGCGG
    TTCCTGACCA CCACACCCAA CTCCTTGCTG GTGTCATGGC AGGCGCCCCG TGCCAGGATT
    ACTGGCTACA TTATCAAGTA TGAGAAGCCT GGATCCCCTC CCAGAGAAGT GGTCCCTCGG
    CCCCGCCCTG GTGTCACGGA GGCCACCATT ACTGGTCTGG AGCCAGGAAC CGAGTACACC
    ATCTACGTCA TTGCCCTGAA GAACAATCAG AAGAGTGAGC CCCTGATTGG GAGGAAGAAG
    ACAGATGAGC TTCCCCAACT GGTTACCCTT CCACACCCCA ATCTTCATGG ACCAGAGATC
    TTGGATGTTC CCTCCACAGT TCAAAAGACC CCCTTCATCA CCAACCCTGG GTATGACACC
    GAAAATGGTA TTCAGCTTCC TGGCACAACC CACCAGCAAC CCAGTGTTGG GCAACAAATG
    ATCTTTGAGG AACATGGCTT TAGGCGGACA ACGCCACCCA CTGCGGCCAC CCCCGTCAGG
    CTTAGGCCAA GACCATACCT GCCGAATGTA GATGAGGAGG TCCAAATCGG TCATGTTCCC
    AGGGGAGACG TAGACTACCA CCTCTATCCT CATGTTCCGG GCCTCAATCC AAATGCCTCT
    ACAGGACAGG AAGCTCTCTC TCAGACAACC ATCTCTTGGA CGCCGTTCCA GGAGAGTTCT
    GAGTACATCA TTTCATGCCA ACCAGTTGGC ACCGACGAAG AGCCCTTACA GTTCCAAGTT
    CCTGGAACTT CTACCAGTGC GACTCTGACT GGCCTTACCA GAGGGGTCAC CTACAACATC
    ATAGTGGAGG CACTGCAGAA CCAGAGGAGG CACAAGGTTC GGGAAGAGGT TGTGACTGTG
    GGCAACGCTG AATGGTGCCA TGACAACGGT GTCAACTACA AGATCGGAGA GAAGTGGGAT
    CGGCAGGGAG AAAATGGCCA GCGGATGAGC TGCACATGCC TCGGGAATGG AAAGGGAGAA
    TTCAAGTGTG ATCCCCATGA AGCAACGTGC TATGACGATG GGAAGACCTA CCATGTAGGA
    GAACAGTGGC AGAAAGAATA TCTCGGAGCC ATTTGCTCCT GCACGTGTTT CGGAGGCCAG
    CGGGGCTGGC GCTGTGACAA CTGCCGTAGA CCTGGGGCTG CTGAACCCAG TCCCGATGGC
    ACCACCGGCC ACACCTACAA CCAGTATACA CAGAGATACA ATCAGAGAAC AAACACTAAC
    GTAAATTGCC CCATTGAGTG CTTCATGCCG CTAGATGTGC AAGCTGACAG AGACGATTCT
    CGAGAGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu76976
    Clone ID Related Accession (Same CDS sequence) XM_017315775.2 , XM_017315775.1
    Accession Version XM_017315775.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7269bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-21
    Organism Mus musculus(house mouse)
    Product fibronectin isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    ATGCTCAGGG GTCCGGGACC CGGGCGGCTG CTGCTGCTGG CAGTCCTGTG CCTGGGGACC 
    TCGGTGCGCT GCACCGAAGC CGGGAAGAGC AAGAGGCAGG CTCAGCAAAT CGTGCAGCCT
    CAATCCCCGG TGGCTGTCAG TCAGAGCAAG CCTGGCTGTT TTGACAATGG GAAGCACTAT
    CAGATAAATC AGCAGTGGGA ACGGACCTAC CTAGGCAACG CCCTGGTTTG TACCTGCTAT
    GGAGGAAGCC GGGGTTTTAA CTGCGAGAGC AAGCCTGAGC CTGAAGAGAC TTGCTTTGAC
    AAATACACTG GGAACACTTA CAAAGTGGGT GACACTTATG AGCGCCCTAA AGATTCCATG
    ATCTGGGACT GTACCTGCAT CGGGGCTGGG AGAGGCAGGA TCAGCTGTAC CATTGCAAAT
    CGCTGCCATG AAGGGGGTCA GTCCTACAAG ATTGGCGACA AGTGGAGGAG GCCACATGAG
    ACTGGTGGCT ACATGTTAGA GTGTCTGTGT CTGGGAAATG GAAAAGGGGA ATGGACCTGC
    AAACCTATAG CTGAGAAGTG TTTTGATCAT GCTGCTGGGA CGTCCTACGT CGTGGGGGAG
    ACCTGGGAAA AGCCCTACCA AGGCTGGATG ATGGTGGACT GTACTTGTCT AGGCGAAGGC
    AATGGACGCA TCACCTGTAC CTCCAGAAAC AGATGCAACG ATCAGGACAC CCGGACATCC
    TATAGGATTG GAGACACGTG GAGCAAGAAG GACAACCGAG GAAACCTGCT TCAGTGTGTC
    TGCACAGGCA ATGGCAGAGG GGAGTGGAAG TGTGAGCGAC ATGCTCTACA AAGTGCTTCA
    GCCGGATCTG GCTCCTTCAC TGATGTCCGA ACAGCTATTT ACCAACCGCA GACTCACCCC
    CAGCCCGCTC CCTACGGCCA CTGTGTCACC GACAGTGGTG TGGTCTACTC TGTGGGAATG
    CAGTGGCTGA AGTCGCAAGG AAACAAGCAA ATGCTGTGCA CGTGCCTGGG CAATGGCGTC
    AGCTGCCAGG AGACAGCCGT GACCCAGACT TATGGTGGCA ATTCAAACGG GGAGCCCTGT
    GTCCTCCCGT TCACCTACAA CGGTAGGACC TTCTATTCCT GCACCACCGA AGGGCGGCAA
    GACGGACATC TGTGGTGTAG CACAACTTCC AATTACGAAC AAGACCAGAA GTATTCCTTC
    TGCACAGACC ATGCGGTTTT GGTTCAGACT CGAGGCGGAA ATTCCAATGG TGCTCTGTGC
    CACTTCCCCT TCCTGTACAA CAACCGGAAT TACACCGACT GTACTTCTGA GGGTCGCAGG
    GACAACATGA AATGGTGCGG CACCACCCAG AACTACGATG CCGATCAGAA GTTTGGATTC
    TGCCCAATGG CTGCCCACGA GGAGATCTGC ACAACCAATG AAGGGGTCAT GTATCGCATT
    GGGGATCAGT GGGATAAGCA GCATGACCTG GGCCACATGA TGAGGTGCAC GTGTGTGGGG
    AACGGTCGTG GAGAATGGGC CTGCATCCCC TACTCCCAGC TCCGAGACCA GTGCATCGTT
    GATGACATTA CTTACAATGT GAACGACACG TTCCACAAGC GTCACGAGGA GGGACATATG
    CTGAACTGTA CCTGCTTTGG TCAGGGCCGG GGCAGATGGA AGTGTGACCC CATTGACCAG
    TGCCAAGATT CAGAGACCCG GACATTTTAC CAGATTGGTG ACTCCTGGGA GAAGTTTGTG
    CATGGTGTCC GATACCAGTG TTACTGCTAC GGCCGTGGCA TCGGGGAGTG GCACTGTCAA
    CCTCTGCAGA CCTACCCAGG CACAACTGGA CCTGTCCAAG TAATTATCAC GGAGACCCCC
    AGCCAGCCCA ATTCCCACCC CATCCAGTGG AATGCCCCGG AGCCTTCACA CATCACCAAG
    TACATTCTCA GATGGAGACC TAAAACCTCT ACGGGTCGCT GGAAGGAAGC TACCATTCCT
    GGCCACCTTA ACTCCTATAC CATCAAAGGC CTGACCCCAG GTGTGATCTA TGAGGGACAG
    CTCATCAGCA TCCAGCAGTA TGGCCACAGA GAAGTGACAC GCTTCGACTT CACCACCAGC
    GCCAGCACCC CTGTGACCAG CAACACGGTG ACCGGAGAGA CTGCGCCCTA CTCTCCTGTT
    GTGGCCACTT CTGAATCTGT AACTGAAATC ACAGCCAGCA GCTTTGTGGT CTCATGGGTC
    TCAGCCTCCG ACACCGTGTC AGGCTTCCGG GTGGAGTATG AGCTGAGCGA GGAGGGAGAT
    GAACCACAGT ACCTTGATCT CCCAAGCACG GCCACTTCCG TGAACATTCC TGACCTGCTC
    CCGGGCAGAA AGTACATTGT CAATGTCTAT CAGATATCTG AGGAGGGAAA ACAGAGCTTG
    ATCCTGTCTA CCTCACAGAC TACAGCACCT GACGCTCCTC CAGACCCTAC CGTGGACCAG
    GTTGATGATA CTTCCATTGT TGTTCGGTGG AGTAGACCCC AGGCACCTAT CACAGGGTAT
    AGAATTGTCT ATTCACCTTC AGTAGAAGGC AGTAGCACAG AGCTCAACCT CCCTGAAACG
    GCCAACTCCG TCACCCTCAG CGACCTGCAG CCCGGTGTTC AGTACAACAT CACTATCTAT
    GCTGTGGAGG AGAACCAGGA GAGCACACCC GTTTTCATCC AACAAGAGAC CACTGGCACC
    CCAAGATCTG ATAACGTCCC CCCTCCGACG GACCTACAGT TTGTGGAACT GACTGATGTG
    AAAGTCACCA TCATGTGGAC CCCTCCTGAT AGTGTGGTGT CTGGATACCG TGTGGAGGTC
    CTGCCTGTCA GCCTGCCCGG GGAACATGGG CAGAGGCTGC CTGTCAACAG AAATACCTTT
    GCTGAAATCA CTGGGCTGTC CCCTGGGGTC ACGTACCTCT TCAAAGTCTT TGCTGTGCAC
    CAGGGCAGGG AAAGCAATCC TCTGACGGCA CAACAGACCA CCAAACTCGA CGCTCCCACT
    AACCTCCAGT TTGTCAATGA AACTGACAGA ACAGTTCTGG TAACGTGGAC TCCACCTCGA
    GCCCGTATAG CAGGCTACCG ACTGACCGCG GGCCTGACCC GAGGAGGCCA GCCCAAGCAG
    TACAATGTGG GACCCTTGGC CTCCAAGTAT CCCCTGAGAA ATCTGCAGCC TGGGTCTGAG
    TACACCGTGA CCTTGGTGGC TGTGAAAGGG AACCAGCAGA GTCCCAAAGC CACCGGAGTC
    TTTACTACCC TGCAGCCTCT GCGCTCCATT CCACCTTACA ACACCGAGGT GACAGAGACC
    ACAATTGTGA TCACCTGGAC CCCCGCTCCA AGGATTGGCT TCAAGCTGGG TGTACGACCG
    AGCCAGGGAG GTGAGGCACC CCGAGAAGTG ACTTCAGACT CTGGGAGCAT TGTTGTGTCT
    GGCTTGACTC CAGGCGTGGA ATACACTTAC ACCATCCAAG TCCTGCGAGA TGGCCAGGAG
    AGAGATGCAC CGATTGTCAA CAGAGTAGTG ACACCGCTGT CTCCACCGAC CAACTTGCAT
    CTGGAGGCAA ACCCTGACAC TGGAGTGCTT ACTGTCTCCT GGGAGAGGAG CACTACCCCA
    GATATCACTG GCTACAGAAT AACTACTACC CCCACGAACG GGCAGCAGGG GACCTCTCTG
    GAAGAAGTGG TCCATGCTGA TCAGAGTTCC TGCACTTTTG AGAACCTGAA TCCTGGCCTG
    GAGTACAACG TCAGTGTTTA CACTGTCAAA GATGACAAGG AAAGTGCCCC TATCTCTGAT
    ACCGTTGTCC CAGAGGTGCC CCAGCTCACT GACCTAAGCT TTGTTGATAT AACTGATTCA
    AGCATCGGCC TGAGGTGGAC CCCGCTAAAC TCTTCCACCA TTATCGGGTA CCGAATCACA
    GTAGTTGCGG CAGGAGAAGG GATCCCTATT TTTGAAGATT TTGTGGACTC CTCAGTAGGA
    TACTACACAG TTACAGGGCT GGAGCCTGGC ATTGACTATG ACATCAGCGT TATCACTCTC
    ATTAATGGCG GAGAGAGTGC CCCTACTACA CTGACACAGC AAACGGCTGT CCCTCCTCCC
    ACGGATCTGC GATTCACCAA TATCGGTCCA GACACGATGC GGGTCACTTG GGCCCCGCCT
    CCGTCCATCG AGCTGACCAA CCTCTTGGTG CGCTACTCAC CCGTGAAGAA TGAAGAGGAC
    GTTGCAGAGC TATCCATTTC ACCTTCAGAC AATGCCGTGG TCCTAACAAA TCTCCTGCCT
    GGGACAGAAT ACTTAGTCAG TGTCTCCAGT GTCTACGAAC AACATGAGAG CATCCCTCTC
    CGGGGAAGGC AGAAAACAGG TCTCGATTCC CCAACTGGTT TTGATTCTTC TGATATCACC
    GCCAACTCAT TCACTGTCCA CTGGGTGGCT CCTCGGGCCC CCATCACCGG CTACATCATC
    CGCCATCACG CCGAGCATTC TGTCGGAAGA CCCAGGCAGG ATCGAGTGCC GCCCTCGCGG
    AATTCCATCA CCCTCACCAA CCTTAATCCG GGCACCGAGT ACGTTGTCAG CATCATTGCT
    GTTAATGGCA GAGAGGAGAG CCCGCCACTG ATTGGCCAGC AAGCCACAGT TTCTGATATT
    CCGAGAGATC TGGAGGTCAT TGCCTCCACC CCCACCAGCC TGCTCATCAG TTGGGAACCC
    CCTGCCGTCT CTGTGCGCTA TTACAGAATC ACCTACGGAG AGACAGGAGG AAATAGCCCT
    GTCCAGGAGT TCACTGTGCC CGGAAGCAAG TCCACAGCCA CCATCAACAA CATTAAACCA
    GGAGCAGACT ACACCATCAC CCTGTATGCT GTCACTGGCC GTGGGGACAG TCCAGCAAGC
    AGCAAGCCAG TTTCCATCAA TTATAAAACA GAAATTGACA AGCCGTCCCA GATGCAGGTG
    ACAGATGTCC AGGACAACAG CATCAGTGTC AGGTGGCTGC CTTCAACTTC TCCTGTGACA
    GGCTACAGAG TGACCACCAC TCCCAAAAAT GGCCTAGGAC CATCAAAAAC TAAAACTGCC
    AGTCCAGATC AAACAGAAAT GACCATTGAA GGTTTGCAAC CCACTGTGGA GTACGTGGTT
    AGTGTTTATG CTCAGAACCG GAACGGAGAA AGCCAGCCCC TGGTTCAAAC TGCAGTGACC
    AACATTGATC GCCCTAAAGG ACTGGCATTC ACTGATGTGG ATGTCGATTC CATCAAAATT
    GCTTGGGAAA GCCCACAGGG GCAAGTTTCC AGGTACAGGG TGACCTACTC GAGCCCTGAG
    GATGGAATCC GGGAGCTTTT CCCTGCACCT GATGGTGAAG ACGACACTGC AGAGCTGCAG
    GGCCTCAGGC CGGGGTCTGA GTACACAGTC AGTGTGGTTG CCTTGCACGA TGATATGGAG
    AGCCAGCCCC TGATTGGAAT CCAGTCCACA GCCATTCCTG CGCCCACCAA TCTGAAGTTC
    AGTCAGGTGA CACCCACCAG CTTTACTGCC CAGTGGATAG CACCCAGTGT TCAGCTCACT
    GGCTACCGGG TGCGGGTGAA CCCGAAAGAG AAGACAGGAC CAATGAAAGA AATCAACCTT
    TCTCCAGACA GCTCATCGGT GATTGTGTCA GGACTCATGG TGGCCACTAA ATACGAAGTC
    AGTGTCTATG CTCTCAAGGA CACACTGACA AGCAGACCAG CCCAGGGAGT CATCACTACT
    CTGGAGAATG TTAGCCCTCC AAGAAGGGCC CGTGTGACGG ACGCTACAGA GACCACCATC
    ACTATTAGCT GGAGAACAAA GACAGAGACA ATCACTGGCT TCCAAGTCGA TGCCATCCCA
    GCCAATGGCC AGACCCCAGT TCAGAGGAGC ATCAGCCCGG ATGTTAGAAG CTACACCATT
    ACAGGTTTAC AGCCAGGCAC TGACTACAAG ATCCACCTGT ACACTCTGAA CGACAATGCC
    CGGAGCTCGC CCGTGATCAT CGATGCCTCC ACCGCCATCG ACGCACCATC CAACCTGCGG
    TTCCTGACCA CCACACCCAA CTCCTTGCTG GTGTCATGGC AGGCGCCCCG TGCCAGGATT
    ACTGGCTACA TTATCAAGTA TGAGAAGCCT GGATCCCCTC CCAGAGAAGT GGTCCCTCGG
    CCCCGCCCTG GTGTCACGGA GGCCACCATT ACTGGTCTGG AGCCAGGAAC CGAGTACACC
    ATCTACGTCA TTGCCCTGAA GAACAATCAG AAGAGTGAGC CCCTGATTGG GAGGAAGAAG
    ACAGATGAGC TTCCCCAACT GGTTACCCTT CCACACCCCA ATCTTCATGG ACCAGAGATC
    TTGGATGTTC CCTCCACAGT TCAAAAGACC CCCTTCATCA CCAACCCTGG GTATGACACC
    GAAAATGGTA TTCAGCTTCC TGGCACAACC CACCAGCAAC CCAGTGTTGG GCAACAAATG
    ATCTTTGAGG AACATGGCTT TAGGCGGACA ACGCCACCCA CTGCGGCCAC CCCCGTCAGG
    CTTAGGCCAA GACCATACCT GCCGAATGTA GATGAGGAGG TCCAAATCGG TCATGTTCCC
    AGGGGAGACG TAGACTACCA CCTCTATCCT CATGTTCCGG GCCTCAATCC AAATGCCTCT
    ACAGGACAGG AAGCTCTCTC TCAGACAACC ATCTCTTGGA CGCCGTTCCA GGAGAGTTCT
    GAGTACATCA TTTCATGCCA ACCAGTTGGC ACCGACGAAG AGCCCTTACA GTTCCAAGTT
    CCTGGAACTT CTACCAGTGC GACTCTGACT GGCCTTACCA GAGGGGTCAC CTACAACATC
    ATAGTGGAGG CACTGCAGAA CCAGAGGAGG CACAAGGTTC GGGAAGAGGT TGTGACTGTG
    GGCAACGCTG AATGGTGCCA TGACAACGGT GTCAACTACA AGATCGGAGA GAAGTGGGAT
    CGGCAGGGAG AAAATGGCCA GCGGATGAGC TGCACATGCC TCGGGAATGG AAAGGGAGAA
    TTCAAGTGTG ATCCCCATGA AGCAACGTGC TATGACGATG GGAAGACCTA CCATGTAGGA
    GAACAGTGGC AGAAAGAATA TCTCGGAGCC ATTTGCTCCT GCACGTGTTT CGGAGGCCAG
    CGGGGCTGGC GCTGTGACAA CTGCCGTAGA CCTGGGGCTG CTGAACCCAG TCCCGATGGC
    ACCACCGGCC ACACCTACAA CCAGTATACA CAGAGATACA ATCAGAGAAC AAACACTAAC
    GTAAATTGCC CCATTGAGTG CTTCATGCCG CTAGATGTGC AAGCTGACAG AGACGATTCT
    CGAGAGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017171264.1
    CDS248..7516
    Translation

    Target ORF information:

    RefSeq Version XM_017315775.1
    Organism Mus musculus(house mouse)
    Definition Mus musculus fibronectin 1 (Fn1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017315775.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    ATGCTCAGGG GTCCGGGACC CGGGCGGCTG CTGCTGCTGG CAGTCCTGTG CCTGGGGACC 
    TCGGTGCGCT GCACCGAAGC CGGGAAGAGC AAGAGGCAGG CTCAGCAAAT CGTGCAGCCT
    CAATCCCCGG TGGCTGTCAG TCAGAGCAAG CCTGGCTGTT TTGACAATGG GAAGCACTAT
    CAGATAAATC AGCAGTGGGA ACGGACCTAC CTAGGCAACG CCCTGGTTTG TACCTGCTAT
    GGAGGAAGCC GGGGTTTTAA CTGCGAGAGC AAGCCTGAGC CTGAAGAGAC TTGCTTTGAC
    AAATACACTG GGAACACTTA CAAAGTGGGT GACACTTATG AGCGCCCTAA AGATTCCATG
    ATCTGGGACT GTACCTGCAT CGGGGCTGGG AGAGGCAGGA TCAGCTGTAC CATTGCAAAT
    CGCTGCCATG AAGGGGGTCA GTCCTACAAG ATTGGCGACA AGTGGAGGAG GCCACATGAG
    ACTGGTGGCT ACATGTTAGA GTGTCTGTGT CTGGGAAATG GAAAAGGGGA ATGGACCTGC
    AAACCTATAG CTGAGAAGTG TTTTGATCAT GCTGCTGGGA CGTCCTACGT CGTGGGGGAG
    ACCTGGGAAA AGCCCTACCA AGGCTGGATG ATGGTGGACT GTACTTGTCT AGGCGAAGGC
    AATGGACGCA TCACCTGTAC CTCCAGAAAC AGATGCAACG ATCAGGACAC CCGGACATCC
    TATAGGATTG GAGACACGTG GAGCAAGAAG GACAACCGAG GAAACCTGCT TCAGTGTGTC
    TGCACAGGCA ATGGCAGAGG GGAGTGGAAG TGTGAGCGAC ATGCTCTACA AAGTGCTTCA
    GCCGGATCTG GCTCCTTCAC TGATGTCCGA ACAGCTATTT ACCAACCGCA GACTCACCCC
    CAGCCCGCTC CCTACGGCCA CTGTGTCACC GACAGTGGTG TGGTCTACTC TGTGGGAATG
    CAGTGGCTGA AGTCGCAAGG AAACAAGCAA ATGCTGTGCA CGTGCCTGGG CAATGGCGTC
    AGCTGCCAGG AGACAGCCGT GACCCAGACT TATGGTGGCA ATTCAAACGG GGAGCCCTGT
    GTCCTCCCGT TCACCTACAA CGGTAGGACC TTCTATTCCT GCACCACCGA AGGGCGGCAA
    GACGGACATC TGTGGTGTAG CACAACTTCC AATTACGAAC AAGACCAGAA GTATTCCTTC
    TGCACAGACC ATGCGGTTTT GGTTCAGACT CGAGGCGGAA ATTCCAATGG TGCTCTGTGC
    CACTTCCCCT TCCTGTACAA CAACCGGAAT TACACCGACT GTACTTCTGA GGGTCGCAGG
    GACAACATGA AATGGTGCGG CACCACCCAG AACTACGATG CCGATCAGAA GTTTGGATTC
    TGCCCAATGG CTGCCCACGA GGAGATCTGC ACAACCAATG AAGGGGTCAT GTATCGCATT
    GGGGATCAGT GGGATAAGCA GCATGACCTG GGCCACATGA TGAGGTGCAC GTGTGTGGGG
    AACGGTCGTG GAGAATGGGC CTGCATCCCC TACTCCCAGC TCCGAGACCA GTGCATCGTT
    GATGACATTA CTTACAATGT GAACGACACG TTCCACAAGC GTCACGAGGA GGGACATATG
    CTGAACTGTA CCTGCTTTGG TCAGGGCCGG GGCAGATGGA AGTGTGACCC CATTGACCAG
    TGCCAAGATT CAGAGACCCG GACATTTTAC CAGATTGGTG ACTCCTGGGA GAAGTTTGTG
    CATGGTGTCC GATACCAGTG TTACTGCTAC GGCCGTGGCA TCGGGGAGTG GCACTGTCAA
    CCTCTGCAGA CCTACCCAGG CACAACTGGA CCTGTCCAAG TAATTATCAC GGAGACCCCC
    AGCCAGCCCA ATTCCCACCC CATCCAGTGG AATGCCCCGG AGCCTTCACA CATCACCAAG
    TACATTCTCA GATGGAGACC TAAAACCTCT ACGGGTCGCT GGAAGGAAGC TACCATTCCT
    GGCCACCTTA ACTCCTATAC CATCAAAGGC CTGACCCCAG GTGTGATCTA TGAGGGACAG
    CTCATCAGCA TCCAGCAGTA TGGCCACAGA GAAGTGACAC GCTTCGACTT CACCACCAGC
    GCCAGCACCC CTGTGACCAG CAACACGGTG ACCGGAGAGA CTGCGCCCTA CTCTCCTGTT
    GTGGCCACTT CTGAATCTGT AACTGAAATC ACAGCCAGCA GCTTTGTGGT CTCATGGGTC
    TCAGCCTCCG ACACCGTGTC AGGCTTCCGG GTGGAGTATG AGCTGAGCGA GGAGGGAGAT
    GAACCACAGT ACCTTGATCT CCCAAGCACG GCCACTTCCG TGAACATTCC TGACCTGCTC
    CCGGGCAGAA AGTACATTGT CAATGTCTAT CAGATATCTG AGGAGGGAAA ACAGAGCTTG
    ATCCTGTCTA CCTCACAGAC TACAGCACCT GACGCTCCTC CAGACCCTAC CGTGGACCAG
    GTTGATGATA CTTCCATTGT TGTTCGGTGG AGTAGACCCC AGGCACCTAT CACAGGGTAT
    AGAATTGTCT ATTCACCTTC AGTAGAAGGC AGTAGCACAG AGCTCAACCT CCCTGAAACG
    GCCAACTCCG TCACCCTCAG CGACCTGCAG CCCGGTGTTC AGTACAACAT CACTATCTAT
    GCTGTGGAGG AGAACCAGGA GAGCACACCC GTTTTCATCC AACAAGAGAC CACTGGCACC
    CCAAGATCTG ATAACGTCCC CCCTCCGACG GACCTACAGT TTGTGGAACT GACTGATGTG
    AAAGTCACCA TCATGTGGAC CCCTCCTGAT AGTGTGGTGT CTGGATACCG TGTGGAGGTC
    CTGCCTGTCA GCCTGCCCGG GGAACATGGG CAGAGGCTGC CTGTCAACAG AAATACCTTT
    GCTGAAATCA CTGGGCTGTC CCCTGGGGTC ACGTACCTCT TCAAAGTCTT TGCTGTGCAC
    CAGGGCAGGG AAAGCAATCC TCTGACGGCA CAACAGACCA CCAAACTCGA CGCTCCCACT
    AACCTCCAGT TTGTCAATGA AACTGACAGA ACAGTTCTGG TAACGTGGAC TCCACCTCGA
    GCCCGTATAG CAGGCTACCG ACTGACCGCG GGCCTGACCC GAGGAGGCCA GCCCAAGCAG
    TACAATGTGG GACCCTTGGC CTCCAAGTAT CCCCTGAGAA ATCTGCAGCC TGGGTCTGAG
    TACACCGTGA CCTTGGTGGC TGTGAAAGGG AACCAGCAGA GTCCCAAAGC CACCGGAGTC
    TTTACTACCC TGCAGCCTCT GCGCTCCATT CCACCTTACA ACACCGAGGT GACAGAGACC
    ACAATTGTGA TCACCTGGAC CCCCGCTCCA AGGATTGGCT TCAAGCTGGG TGTACGACCG
    AGCCAGGGAG GTGAGGCACC CCGAGAAGTG ACTTCAGACT CTGGGAGCAT TGTTGTGTCT
    GGCTTGACTC CAGGCGTGGA ATACACTTAC ACCATCCAAG TCCTGCGAGA TGGCCAGGAG
    AGAGATGCAC CGATTGTCAA CAGAGTAGTG ACACCGCTGT CTCCACCGAC CAACTTGCAT
    CTGGAGGCAA ACCCTGACAC TGGAGTGCTT ACTGTCTCCT GGGAGAGGAG CACTACCCCA
    GATATCACTG GCTACAGAAT AACTACTACC CCCACGAACG GGCAGCAGGG GACCTCTCTG
    GAAGAAGTGG TCCATGCTGA TCAGAGTTCC TGCACTTTTG AGAACCTGAA TCCTGGCCTG
    GAGTACAACG TCAGTGTTTA CACTGTCAAA GATGACAAGG AAAGTGCCCC TATCTCTGAT
    ACCGTTGTCC CAGAGGTGCC CCAGCTCACT GACCTAAGCT TTGTTGATAT AACTGATTCA
    AGCATCGGCC TGAGGTGGAC CCCGCTAAAC TCTTCCACCA TTATCGGGTA CCGAATCACA
    GTAGTTGCGG CAGGAGAAGG GATCCCTATT TTTGAAGATT TTGTGGACTC CTCAGTAGGA
    TACTACACAG TTACAGGGCT GGAGCCTGGC ATTGACTATG ACATCAGCGT TATCACTCTC
    ATTAATGGCG GAGAGAGTGC CCCTACTACA CTGACACAGC AAACGGCTGT CCCTCCTCCC
    ACGGATCTGC GATTCACCAA TATCGGTCCA GACACGATGC GGGTCACTTG GGCCCCGCCT
    CCGTCCATCG AGCTGACCAA CCTCTTGGTG CGCTACTCAC CCGTGAAGAA TGAAGAGGAC
    GTTGCAGAGC TATCCATTTC ACCTTCAGAC AATGCCGTGG TCCTAACAAA TCTCCTGCCT
    GGGACAGAAT ACTTAGTCAG TGTCTCCAGT GTCTACGAAC AACATGAGAG CATCCCTCTC
    CGGGGAAGGC AGAAAACAGG TCTCGATTCC CCAACTGGTT TTGATTCTTC TGATATCACC
    GCCAACTCAT TCACTGTCCA CTGGGTGGCT CCTCGGGCCC CCATCACCGG CTACATCATC
    CGCCATCACG CCGAGCATTC TGTCGGAAGA CCCAGGCAGG ATCGAGTGCC GCCCTCGCGG
    AATTCCATCA CCCTCACCAA CCTTAATCCG GGCACCGAGT ACGTTGTCAG CATCATTGCT
    GTTAATGGCA GAGAGGAGAG CCCGCCACTG ATTGGCCAGC AAGCCACAGT TTCTGATATT
    CCGAGAGATC TGGAGGTCAT TGCCTCCACC CCCACCAGCC TGCTCATCAG TTGGGAACCC
    CCTGCCGTCT CTGTGCGCTA TTACAGAATC ACCTACGGAG AGACAGGAGG AAATAGCCCT
    GTCCAGGAGT TCACTGTGCC CGGAAGCAAG TCCACAGCCA CCATCAACAA CATTAAACCA
    GGAGCAGACT ACACCATCAC CCTGTATGCT GTCACTGGCC GTGGGGACAG TCCAGCAAGC
    AGCAAGCCAG TTTCCATCAA TTATAAAACA GAAATTGACA AGCCGTCCCA GATGCAGGTG
    ACAGATGTCC AGGACAACAG CATCAGTGTC AGGTGGCTGC CTTCAACTTC TCCTGTGACA
    GGCTACAGAG TGACCACCAC TCCCAAAAAT GGCCTAGGAC CATCAAAAAC TAAAACTGCC
    AGTCCAGATC AAACAGAAAT GACCATTGAA GGTTTGCAAC CCACTGTGGA GTACGTGGTT
    AGTGTTTATG CTCAGAACCG GAACGGAGAA AGCCAGCCCC TGGTTCAAAC TGCAGTGACC
    AACATTGATC GCCCTAAAGG ACTGGCATTC ACTGATGTGG ATGTCGATTC CATCAAAATT
    GCTTGGGAAA GCCCACAGGG GCAAGTTTCC AGGTACAGGG TGACCTACTC GAGCCCTGAG
    GATGGAATCC GGGAGCTTTT CCCTGCACCT GATGGTGAAG ACGACACTGC AGAGCTGCAG
    GGCCTCAGGC CGGGGTCTGA GTACACAGTC AGTGTGGTTG CCTTGCACGA TGATATGGAG
    AGCCAGCCCC TGATTGGAAT CCAGTCCACA GCCATTCCTG CGCCCACCAA TCTGAAGTTC
    AGTCAGGTGA CACCCACCAG CTTTACTGCC CAGTGGATAG CACCCAGTGT TCAGCTCACT
    GGCTACCGGG TGCGGGTGAA CCCGAAAGAG AAGACAGGAC CAATGAAAGA AATCAACCTT
    TCTCCAGACA GCTCATCGGT GATTGTGTCA GGACTCATGG TGGCCACTAA ATACGAAGTC
    AGTGTCTATG CTCTCAAGGA CACACTGACA AGCAGACCAG CCCAGGGAGT CATCACTACT
    CTGGAGAATG TTAGCCCTCC AAGAAGGGCC CGTGTGACGG ACGCTACAGA GACCACCATC
    ACTATTAGCT GGAGAACAAA GACAGAGACA ATCACTGGCT TCCAAGTCGA TGCCATCCCA
    GCCAATGGCC AGACCCCAGT TCAGAGGAGC ATCAGCCCGG ATGTTAGAAG CTACACCATT
    ACAGGTTTAC AGCCAGGCAC TGACTACAAG ATCCACCTGT ACACTCTGAA CGACAATGCC
    CGGAGCTCGC CCGTGATCAT CGATGCCTCC ACCGCCATCG ACGCACCATC CAACCTGCGG
    TTCCTGACCA CCACACCCAA CTCCTTGCTG GTGTCATGGC AGGCGCCCCG TGCCAGGATT
    ACTGGCTACA TTATCAAGTA TGAGAAGCCT GGATCCCCTC CCAGAGAAGT GGTCCCTCGG
    CCCCGCCCTG GTGTCACGGA GGCCACCATT ACTGGTCTGG AGCCAGGAAC CGAGTACACC
    ATCTACGTCA TTGCCCTGAA GAACAATCAG AAGAGTGAGC CCCTGATTGG GAGGAAGAAG
    ACAGATGAGC TTCCCCAACT GGTTACCCTT CCACACCCCA ATCTTCATGG ACCAGAGATC
    TTGGATGTTC CCTCCACAGT TCAAAAGACC CCCTTCATCA CCAACCCTGG GTATGACACC
    GAAAATGGTA TTCAGCTTCC TGGCACAACC CACCAGCAAC CCAGTGTTGG GCAACAAATG
    ATCTTTGAGG AACATGGCTT TAGGCGGACA ACGCCACCCA CTGCGGCCAC CCCCGTCAGG
    CTTAGGCCAA GACCATACCT GCCGAATGTA GATGAGGAGG TCCAAATCGG TCATGTTCCC
    AGGGGAGACG TAGACTACCA CCTCTATCCT CATGTTCCGG GCCTCAATCC AAATGCCTCT
    ACAGGACAGG AAGCTCTCTC TCAGACAACC ATCTCTTGGA CGCCGTTCCA GGAGAGTTCT
    GAGTACATCA TTTCATGCCA ACCAGTTGGC ACCGACGAAG AGCCCTTACA GTTCCAAGTT
    CCTGGAACTT CTACCAGTGC GACTCTGACT GGCCTTACCA GAGGGGTCAC CTACAACATC
    ATAGTGGAGG CACTGCAGAA CCAGAGGAGG CACAAGGTTC GGGAAGAGGT TGTGACTGTG
    GGCAACGCTG AATGGTGCCA TGACAACGGT GTCAACTACA AGATCGGAGA GAAGTGGGAT
    CGGCAGGGAG AAAATGGCCA GCGGATGAGC TGCACATGCC TCGGGAATGG AAAGGGAGAA
    TTCAAGTGTG ATCCCCATGA AGCAACGTGC TATGACGATG GGAAGACCTA CCATGTAGGA
    GAACAGTGGC AGAAAGAATA TCTCGGAGCC ATTTGCTCCT GCACGTGTTT CGGAGGCCAG
    CGGGGCTGGC GCTGTGACAA CTGCCGTAGA CCTGGGGCTG CTGAACCCAG TCCCGATGGC
    ACCACCGGCC ACACCTACAA CCAGTATACA CAGAGATACA ATCAGAGAAC AAACACTAAC
    GTAAATTGCC CCATTGAGTG CTTCATGCCG CTAGATGTGC AAGCTGACAG AGACGATTCT
    CGAGAGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu76977
    Clone ID Related Accession (Same CDS sequence) XM_017315837.2 , XM_017315837.1
    Accession Version XM_017315837.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6804bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product fibronectin isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_017315837.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    ATGCTCAGGG GTCCGGGACC CGGGCGGCTG CTGCTGCTGG CAGTCCTGTG CCTGGGGACC 
    TCGGTGCGCT GCACCGAAGC CGGGAAGAGC AAGAGGCAGG CTCAGCAAAT CGTGCAGCCT
    CAATCCCCGG TGGCTGTCAG TCAGAGCAAG CCTGGCTGTT TTGACAATGG GAAGCACTAT
    CAGATAAATC AGCAGTGGGA ACGGACCTAC CTAGGCAACG CCCTGGTTTG TACCTGCTAT
    GGAGGAAGCC GGGGTTTTAA CTGCGAGAGC AAGCCTGAGC CTGAAGAGAC TTGCTTTGAC
    AAATACACTG GGAACACTTA CAAAGTGGGT GACACTTATG AGCGCCCTAA AGATTCCATG
    ATCTGGGACT GTACCTGCAT CGGGGCTGGG AGAGGCAGGA TCAGCTGTAC CATTGCAAAT
    CGCTGCCATG AAGGGGGTCA GTCCTACAAG ATTGGCGACA AGTGGAGGAG GCCACATGAG
    ACTGGTGGCT ACATGTTAGA GTGTCTGTGT CTGGGAAATG GAAAAGGGGA ATGGACCTGC
    AAACCTATAG CTGAGAAGTG TTTTGATCAT GCTGCTGGGA CGTCCTACGT CGTGGGGGAG
    ACCTGGGAAA AGCCCTACCA AGGCTGGATG ATGGTGGACT GTACTTGTCT AGGCGAAGGC
    AATGGACGCA TCACCTGTAC CTCCAGAAAC AGATGCAACG ATCAGGACAC CCGGACATCC
    TATAGGATTG GAGACACGTG GAGCAAGAAG GACAACCGAG GAAACCTGCT TCAGTGTGTC
    TGCACAGGCA ATGGCAGAGG GGAGTGGAAG TGTGAGCGAC ATGCTCTACA AAGTGCTTCA
    GCCGGATCTG GCTCCTTCAC TGATGTCCGA ACAGCTATTT ACCAACCGCA GACTCACCCC
    CAGCCCGCTC CCTACGGCCA CTGTGTCACC GACAGTGGTG TGGTCTACTC TGTGGGAATG
    CAGTGGCTGA AGTCGCAAGG AAACAAGCAA ATGCTGTGCA CGTGCCTGGG CAATGGCGTC
    AGCTGCCAGG AGACAGCCGT GACCCAGACT TATGGTGGCA ATTCAAACGG GGAGCCCTGT
    GTCCTCCCGT TCACCTACAA CGGTAGGACC TTCTATTCCT GCACCACCGA AGGGCGGCAA
    GACGGACATC TGTGGTGTAG CACAACTTCC AATTACGAAC AAGACCAGAA GTATTCCTTC
    TGCACAGACC ATGCGGTTTT GGTTCAGACT CGAGGCGGAA ATTCCAATGG TGCTCTGTGC
    CACTTCCCCT TCCTGTACAA CAACCGGAAT TACACCGACT GTACTTCTGA GGGTCGCAGG
    GACAACATGA AATGGTGCGG CACCACCCAG AACTACGATG CCGATCAGAA GTTTGGATTC
    TGCCCAATGG CTGCCCACGA GGAGATCTGC ACAACCAATG AAGGGGTCAT GTATCGCATT
    GGGGATCAGT GGGATAAGCA GCATGACCTG GGCCACATGA TGAGGTGCAC GTGTGTGGGG
    AACGGTCGTG GAGAATGGGC CTGCATCCCC TACTCCCAGC TCCGAGACCA GTGCATCGTT
    GATGACATTA CTTACAATGT GAACGACACG TTCCACAAGC GTCACGAGGA GGGACATATG
    CTGAACTGTA CCTGCTTTGG TCAGGGCCGG GGCAGATGGA AGTGTGACCC CATTGACCAG
    TGCCAAGATT CAGAGACCCG GACATTTTAC CAGATTGGTG ACTCCTGGGA GAAGTTTGTG
    CATGGTGTCC GATACCAGTG TTACTGCTAC GGCCGTGGCA TCGGGGAGTG GCACTGTCAA
    CCTCTGCAGA CCTACCCAGG CACAACTGGA CCTGTCCAAG TAATTATCAC GGAGACCCCC
    AGCCAGCCCA ATTCCCACCC CATCCAGTGG AATGCCCCGG AGCCTTCACA CATCACCAAG
    TACATTCTCA GATGGAGACC TAAAACCTCT ACGGGTCGCT GGAAGGAAGC TACCATTCCT
    GGCCACCTTA ACTCCTATAC CATCAAAGGC CTGACCCCAG GTGTGATCTA TGAGGGACAG
    CTCATCAGCA TCCAGCAGTA TGGCCACAGA GAAGTGACAC GCTTCGACTT CACCACCAGC
    GCCAGCACCC CTGTGACCAG CAACACGGTG ACCGGAGAGA CTGCGCCCTA CTCTCCTGTT
    GTGGCCACTT CTGAATCTGT AACTGAAATC ACAGCCAGCA GCTTTGTGGT CTCATGGGTC
    TCAGCCTCCG ACACCGTGTC AGGCTTCCGG GTGGAGTATG AGCTGAGCGA GGAGGGAGAT
    GAACCACAGT ACCTTGATCT CCCAAGCACG GCCACTTCCG TGAACATTCC TGACCTGCTC
    CCGGGCAGAA AGTACATTGT CAATGTCTAT CAGATATCTG AGGAGGGAAA ACAGAGCTTG
    ATCCTGTCTA CCTCACAGAC TACAGCACCT GACGCTCCTC CAGACCCTAC CGTGGACCAG
    GTTGATGATA CTTCCATTGT TGTTCGGTGG AGTAGACCCC AGGCACCTAT CACAGGGTAT
    AGAATTGTCT ATTCACCTTC AGTAGAAGGC AGTAGCACAG AGCTCAACCT CCCTGAAACG
    GCCAACTCCG TCACCCTCAG CGACCTGCAG CCCGGTGTTC AGTACAACAT CACTATCTAT
    GCTGTGGAGG AGAACCAGGA GAGCACACCC GTTTTCATCC AACAAGAGAC CACTGGCACC
    CCAAGATCTG ATAACGTCCC CCCTCCGACG GACCTACAGT TTGTGGAACT GACTGATGTG
    AAAGTCACCA TCATGTGGAC CCCTCCTGAT AGTGTGGTGT CTGGATACCG TGTGGAGGTC
    CTGCCTGTCA GCCTGCCCGG GGAACATGGG CAGAGGCTGC CTGTCAACAG AAATACCTTT
    GCTGAAATCA CTGGGCTGTC CCCTGGGGTC ACGTACCTCT TCAAAGTCTT TGCTGTGCAC
    CAGGGCAGGG AAAGCAATCC TCTGACGGCA CAACAGACCA CCAAACTCGA CGCTCCCACT
    AACCTCCAGT TTGTCAATGA AACTGACAGA ACAGTTCTGG TAACGTGGAC TCCACCTCGA
    GCCCGTATAG CAGGCTACCG ACTGACCGCG GGCCTGACCC GAGGAGGCCA GCCCAAGCAG
    TACAATGTGG GACCCTTGGC CTCCAAGTAT CCCCTGAGAA ATCTGCAGCC TGGGTCTGAG
    TACACCGTGA CCTTGGTGGC TGTGAAAGGG AACCAGCAGA GTCCCAAAGC CACCGGAGTC
    TTTACTACCC TGCAGCCTCT GCGCTCCATT CCACCTTACA ACACCGAGGT GACAGAGACC
    ACAATTGTGA TCACCTGGAC CCCCGCTCCA AGGATTGGCT TCAAGCTGGG TGTACGACCG
    AGCCAGGGAG GTGAGGCACC CCGAGAAGTG ACTTCAGACT CTGGGAGCAT TGTTGTGTCT
    GGCTTGACTC CAGGCGTGGA ATACACTTAC ACCATCCAAG TCCTGCGAGA TGGCCAGGAG
    AGAGATGCAC CGATTGTCAA CAGAGTAGTG ACACCGCTGT CTCCACCGAC CAACTTGCAT
    CTGGAGGCAA ACCCTGACAC TGGAGTGCTT ACTGTCTCCT GGGAGAGGAG CACTACCCCA
    GATATCACTG GCTACAGAAT AACTACTACC CCCACGAACG GGCAGCAGGG GACCTCTCTG
    GAAGAAGTGG TCCATGCTGA TCAGAGTTCC TGCACTTTTG AGAACCTGAA TCCTGGCCTG
    GAGTACAACG TCAGTGTTTA CACTGTCAAA GATGACAAGG AAAGTGCCCC TATCTCTGAT
    ACCGTTGTCC CAGAGGTGCC CCAGCTCACT GACCTAAGCT TTGTTGATAT AACTGATTCA
    AGCATCGGCC TGAGGTGGAC CCCGCTAAAC TCTTCCACCA TTATCGGGTA CCGAATCACA
    GTAGTTGCGG CAGGAGAAGG GATCCCTATT TTTGAAGATT TTGTGGACTC CTCAGTAGGA
    TACTACACAG TTACAGGGCT GGAGCCTGGC ATTGACTATG ACATCAGCGT TATCACTCTC
    ATTAATGGCG GAGAGAGTGC CCCTACTACA CTGACACAGC AAACGGCTGT CCCTCCTCCC
    ACGGATCTGC GATTCACCAA TATCGGTCCA GACACGATGC GGGTCACTTG GGCCCCGCCT
    CCGTCCATCG AGCTGACCAA CCTCTTGGTG CGCTACTCAC CCGTGAAGAA TGAAGAGGAC
    GTTGCAGAGC TATCCATTTC ACCTTCAGAC AATGCCGTGG TCCTAACAAA TCTCCTGCCT
    GGGACAGAAT ACTTAGTCAG TGTCTCCAGT GTCTACGAAC AACATGAGAG CATCCCTCTC
    CGGGGAAGGC AGAAAACAGG TCTCGATTCC CCAACTGGTT TTGATTCTTC TGATATCACC
    GCCAACTCAT TCACTGTCCA CTGGGTGGCT CCTCGGGCCC CCATCACCGG CTACATCATC
    CGCCATCACG CCGAGCATTC TGTCGGAAGA CCCAGGCAGG ATCGAGTGCC GCCCTCGCGG
    AATTCCATCA CCCTCACCAA CCTTAATCCG GGCACCGAGT ACGTTGTCAG CATCATTGCT
    GTTAATGGCA GAGAGGAGAG CCCGCCACTG ATTGGCCAGC AAGCCACAGT TTCTGATATT
    CCGAGAGATC TGGAGGTCAT TGCCTCCACC CCCACCAGCC TGCTCATCAG TTGGGAACCC
    CCTGCCGTCT CTGTGCGCTA TTACAGAATC ACCTACGGAG AGACAGGAGG AAATAGCCCT
    GTCCAGGAGT TCACTGTGCC CGGAAGCAAG TCCACAGCCA CCATCAACAA CATTAAACCA
    GGAGCAGACT ACACCATCAC CCTGTATGCT GTCACTGGCC GTGGGGACAG TCCAGCAAGC
    AGCAAGCCAG TTTCCATCAA TTATAAAACA GAAATTGACA AGCCGTCCCA GATGCAGGTG
    ACAGATGTCC AGGACAACAG CATCAGTGTC AGGTGGCTGC CTTCAACTTC TCCTGTGACA
    GGCTACAGAG TGACCACCAC TCCCAAAAAT GGCCTAGGAC CATCAAAAAC TAAAACTGCC
    AGTCCAGATC AAACAGAAAT GACCATTGAA GGTTTGCAAC CCACTGTGGA GTACGTGGTT
    AGTGTTTATG CTCAGAACCG GAACGGAGAA AGCCAGCCCC TGGTTCAAAC TGCAGTGACC
    ACCATTCCTG CGCCCACCAA TCTGAAGTTC AGTCAGGTGA CACCCACCAG CTTTACTGCC
    CAGTGGATAG CACCCAGTGT TCAGCTCACT GGCTACCGGG TGCGGGTGAA CCCGAAAGAG
    AAGACAGGAC CAATGAAAGA AATCAACCTT TCTCCAGACA GCTCATCGGT GATTGTGTCA
    GGACTCATGG TGGCCACTAA ATACGAAGTC AGTGTCTATG CTCTCAAGGA CACACTGACA
    AGCAGACCAG CCCAGGGAGT CATCACTACT CTGGAGAATG TTAGCCCTCC AAGAAGGGCC
    CGTGTGACGG ACGCTACAGA GACCACCATC ACTATTAGCT GGAGAACAAA GACAGAGACA
    ATCACTGGCT TCCAAGTCGA TGCCATCCCA GCCAATGGCC AGACCCCAGT TCAGAGGAGC
    ATCAGCCCGG ATGTTAGAAG CTACACCATT ACAGGTTTAC AGCCAGGCAC TGACTACAAG
    ATCCACCTGT ACACTCTGAA CGACAATGCC CGGAGCTCGC CCGTGATCAT CGATGCCTCC
    ACCGCCATCG ACGCACCATC CAACCTGCGG TTCCTGACCA CCACACCCAA CTCCTTGCTG
    GTGTCATGGC AGGCGCCCCG TGCCAGGATT ACTGGCTACA TTATCAAGTA TGAGAAGCCT
    GGATCCCCTC CCAGAGAAGT GGTCCCTCGG CCCCGCCCTG GTGTCACGGA GGCCACCATT
    ACTGGTCTGG AGCCAGGAAC CGAGTACACC ATCTACGTCA TTGCCCTGAA GAACAATCAG
    AAGAGTGAGC CCCTGATTGG GAGGAAGAAG ACAGGACAGG AAGCTCTCTC TCAGACAACC
    ATCTCTTGGA CGCCGTTCCA GGAGAGTTCT GAGTACATCA TTTCATGCCA ACCAGTTGGC
    ACCGACGAAG AGCCCTTACA GTTCCAAGTT CCTGGAACTT CTACCAGTGC GACTCTGACT
    GGCCTTACCA GAGGGGTCAC CTACAACATC ATAGTGGAGG CACTGCAGAA CCAGAGGAGG
    CACAAGGTTC GGGAAGAGGT TGTGACTGTG GGCAACGCTG TCAGCGAAGG CCTGAACCAG
    CCTACAGATG ACTCATGCTT TGACCCTTAC ACGGTTTCCC ATTACGCCAT TGGAGAGGAG
    TGGGAGCGGT TGTCTGACGC TGGCTTTAAG CTCACATGCC AGTGCTTGGG CTTTGGCAGT
    GGTCATTTCA GATGCGATTC ATCTAAATGG TGCCATGACA ACGGTGTCAA CTACAAGATC
    GGAGAGAAGT GGGATCGGCA GGGAGAAAAT GGCCAGCGGA TGAGCTGCAC ATGCCTCGGG
    AATGGAAAGG GAGAATTCAA GTGTGATCCC CATGAAGCAA CGTGCTATGA CGATGGGAAG
    ACCTACCATG TAGGAGAACA GTGGCAGAAA GAATATCTCG GAGCCATTTG CTCCTGCACG
    TGTTTCGGAG GCCAGCGGGG CTGGCGCTGT GACAACTGCC GTAGACCTGG GGCTGCTGAA
    CCCAGTCCCG ATGGCACCAC CGGCCACACC TACAACCAGT ATACACAGAG ATACAATCAG
    AGAACAAACA CTAACGTAAA TTGCCCCATT GAGTGCTTCA TGCCGCTAGA TGTGCAAGCT
    GACAGAGACG ATTCTCGAGA GTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017171326.1
    CDS299..7102
    Translation

    Target ORF information:

    RefSeq Version XM_017315837.2
    Organism Mus musculus(house mouse)
    Definition Mus musculus fibronectin 1 (Fn1), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017315837.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    ATGCTCAGGG GTCCGGGACC CGGGCGGCTG CTGCTGCTGG CAGTCCTGTG CCTGGGGACC 
    TCGGTGCGCT GCACCGAAGC CGGGAAGAGC AAGAGGCAGG CTCAGCAAAT CGTGCAGCCT
    CAATCCCCGG TGGCTGTCAG TCAGAGCAAG CCTGGCTGTT TTGACAATGG GAAGCACTAT
    CAGATAAATC AGCAGTGGGA ACGGACCTAC CTAGGCAACG CCCTGGTTTG TACCTGCTAT
    GGAGGAAGCC GGGGTTTTAA CTGCGAGAGC AAGCCTGAGC CTGAAGAGAC TTGCTTTGAC
    AAATACACTG GGAACACTTA CAAAGTGGGT GACACTTATG AGCGCCCTAA AGATTCCATG
    ATCTGGGACT GTACCTGCAT CGGGGCTGGG AGAGGCAGGA TCAGCTGTAC CATTGCAAAT
    CGCTGCCATG AAGGGGGTCA GTCCTACAAG ATTGGCGACA AGTGGAGGAG GCCACATGAG
    ACTGGTGGCT ACATGTTAGA GTGTCTGTGT CTGGGAAATG GAAAAGGGGA ATGGACCTGC
    AAACCTATAG CTGAGAAGTG TTTTGATCAT GCTGCTGGGA CGTCCTACGT CGTGGGGGAG
    ACCTGGGAAA AGCCCTACCA AGGCTGGATG ATGGTGGACT GTACTTGTCT AGGCGAAGGC
    AATGGACGCA TCACCTGTAC CTCCAGAAAC AGATGCAACG ATCAGGACAC CCGGACATCC
    TATAGGATTG GAGACACGTG GAGCAAGAAG GACAACCGAG GAAACCTGCT TCAGTGTGTC
    TGCACAGGCA ATGGCAGAGG GGAGTGGAAG TGTGAGCGAC ATGCTCTACA AAGTGCTTCA
    GCCGGATCTG GCTCCTTCAC TGATGTCCGA ACAGCTATTT ACCAACCGCA GACTCACCCC
    CAGCCCGCTC CCTACGGCCA CTGTGTCACC GACAGTGGTG TGGTCTACTC TGTGGGAATG
    CAGTGGCTGA AGTCGCAAGG AAACAAGCAA ATGCTGTGCA CGTGCCTGGG CAATGGCGTC
    AGCTGCCAGG AGACAGCCGT GACCCAGACT TATGGTGGCA ATTCAAACGG GGAGCCCTGT
    GTCCTCCCGT TCACCTACAA CGGTAGGACC TTCTATTCCT GCACCACCGA AGGGCGGCAA
    GACGGACATC TGTGGTGTAG CACAACTTCC AATTACGAAC AAGACCAGAA GTATTCCTTC
    TGCACAGACC ATGCGGTTTT GGTTCAGACT CGAGGCGGAA ATTCCAATGG TGCTCTGTGC
    CACTTCCCCT TCCTGTACAA CAACCGGAAT TACACCGACT GTACTTCTGA GGGTCGCAGG
    GACAACATGA AATGGTGCGG CACCACCCAG AACTACGATG CCGATCAGAA GTTTGGATTC
    TGCCCAATGG CTGCCCACGA GGAGATCTGC ACAACCAATG AAGGGGTCAT GTATCGCATT
    GGGGATCAGT GGGATAAGCA GCATGACCTG GGCCACATGA TGAGGTGCAC GTGTGTGGGG
    AACGGTCGTG GAGAATGGGC CTGCATCCCC TACTCCCAGC TCCGAGACCA GTGCATCGTT
    GATGACATTA CTTACAATGT GAACGACACG TTCCACAAGC GTCACGAGGA GGGACATATG
    CTGAACTGTA CCTGCTTTGG TCAGGGCCGG GGCAGATGGA AGTGTGACCC CATTGACCAG
    TGCCAAGATT CAGAGACCCG GACATTTTAC CAGATTGGTG ACTCCTGGGA GAAGTTTGTG
    CATGGTGTCC GATACCAGTG TTACTGCTAC GGCCGTGGCA TCGGGGAGTG GCACTGTCAA
    CCTCTGCAGA CCTACCCAGG CACAACTGGA CCTGTCCAAG TAATTATCAC GGAGACCCCC
    AGCCAGCCCA ATTCCCACCC CATCCAGTGG AATGCCCCGG AGCCTTCACA CATCACCAAG
    TACATTCTCA GATGGAGACC TAAAACCTCT ACGGGTCGCT GGAAGGAAGC TACCATTCCT
    GGCCACCTTA ACTCCTATAC CATCAAAGGC CTGACCCCAG GTGTGATCTA TGAGGGACAG
    CTCATCAGCA TCCAGCAGTA TGGCCACAGA GAAGTGACAC GCTTCGACTT CACCACCAGC
    GCCAGCACCC CTGTGACCAG CAACACGGTG ACCGGAGAGA CTGCGCCCTA CTCTCCTGTT
    GTGGCCACTT CTGAATCTGT AACTGAAATC ACAGCCAGCA GCTTTGTGGT CTCATGGGTC
    TCAGCCTCCG ACACCGTGTC AGGCTTCCGG GTGGAGTATG AGCTGAGCGA GGAGGGAGAT
    GAACCACAGT ACCTTGATCT CCCAAGCACG GCCACTTCCG TGAACATTCC TGACCTGCTC
    CCGGGCAGAA AGTACATTGT CAATGTCTAT CAGATATCTG AGGAGGGAAA ACAGAGCTTG
    ATCCTGTCTA CCTCACAGAC TACAGCACCT GACGCTCCTC CAGACCCTAC CGTGGACCAG
    GTTGATGATA CTTCCATTGT TGTTCGGTGG AGTAGACCCC AGGCACCTAT CACAGGGTAT
    AGAATTGTCT ATTCACCTTC AGTAGAAGGC AGTAGCACAG AGCTCAACCT CCCTGAAACG
    GCCAACTCCG TCACCCTCAG CGACCTGCAG CCCGGTGTTC AGTACAACAT CACTATCTAT
    GCTGTGGAGG AGAACCAGGA GAGCACACCC GTTTTCATCC AACAAGAGAC CACTGGCACC
    CCAAGATCTG ATAACGTCCC CCCTCCGACG GACCTACAGT TTGTGGAACT GACTGATGTG
    AAAGTCACCA TCATGTGGAC CCCTCCTGAT AGTGTGGTGT CTGGATACCG TGTGGAGGTC
    CTGCCTGTCA GCCTGCCCGG GGAACATGGG CAGAGGCTGC CTGTCAACAG AAATACCTTT
    GCTGAAATCA CTGGGCTGTC CCCTGGGGTC ACGTACCTCT TCAAAGTCTT TGCTGTGCAC
    CAGGGCAGGG AAAGCAATCC TCTGACGGCA CAACAGACCA CCAAACTCGA CGCTCCCACT
    AACCTCCAGT TTGTCAATGA AACTGACAGA ACAGTTCTGG TAACGTGGAC TCCACCTCGA
    GCCCGTATAG CAGGCTACCG ACTGACCGCG GGCCTGACCC GAGGAGGCCA GCCCAAGCAG
    TACAATGTGG GACCCTTGGC CTCCAAGTAT CCCCTGAGAA ATCTGCAGCC TGGGTCTGAG
    TACACCGTGA CCTTGGTGGC TGTGAAAGGG AACCAGCAGA GTCCCAAAGC CACCGGAGTC
    TTTACTACCC TGCAGCCTCT GCGCTCCATT CCACCTTACA ACACCGAGGT GACAGAGACC
    ACAATTGTGA TCACCTGGAC CCCCGCTCCA AGGATTGGCT TCAAGCTGGG TGTACGACCG
    AGCCAGGGAG GTGAGGCACC CCGAGAAGTG ACTTCAGACT CTGGGAGCAT TGTTGTGTCT
    GGCTTGACTC CAGGCGTGGA ATACACTTAC ACCATCCAAG TCCTGCGAGA TGGCCAGGAG
    AGAGATGCAC CGATTGTCAA CAGAGTAGTG ACACCGCTGT CTCCACCGAC CAACTTGCAT
    CTGGAGGCAA ACCCTGACAC TGGAGTGCTT ACTGTCTCCT GGGAGAGGAG CACTACCCCA
    GATATCACTG GCTACAGAAT AACTACTACC CCCACGAACG GGCAGCAGGG GACCTCTCTG
    GAAGAAGTGG TCCATGCTGA TCAGAGTTCC TGCACTTTTG AGAACCTGAA TCCTGGCCTG
    GAGTACAACG TCAGTGTTTA CACTGTCAAA GATGACAAGG AAAGTGCCCC TATCTCTGAT
    ACCGTTGTCC CAGAGGTGCC CCAGCTCACT GACCTAAGCT TTGTTGATAT AACTGATTCA
    AGCATCGGCC TGAGGTGGAC CCCGCTAAAC TCTTCCACCA TTATCGGGTA CCGAATCACA
    GTAGTTGCGG CAGGAGAAGG GATCCCTATT TTTGAAGATT TTGTGGACTC CTCAGTAGGA
    TACTACACAG TTACAGGGCT GGAGCCTGGC ATTGACTATG ACATCAGCGT TATCACTCTC
    ATTAATGGCG GAGAGAGTGC CCCTACTACA CTGACACAGC AAACGGCTGT CCCTCCTCCC
    ACGGATCTGC GATTCACCAA TATCGGTCCA GACACGATGC GGGTCACTTG GGCCCCGCCT
    CCGTCCATCG AGCTGACCAA CCTCTTGGTG CGCTACTCAC CCGTGAAGAA TGAAGAGGAC
    GTTGCAGAGC TATCCATTTC ACCTTCAGAC AATGCCGTGG TCCTAACAAA TCTCCTGCCT
    GGGACAGAAT ACTTAGTCAG TGTCTCCAGT GTCTACGAAC AACATGAGAG CATCCCTCTC
    CGGGGAAGGC AGAAAACAGG TCTCGATTCC CCAACTGGTT TTGATTCTTC TGATATCACC
    GCCAACTCAT TCACTGTCCA CTGGGTGGCT CCTCGGGCCC CCATCACCGG CTACATCATC
    CGCCATCACG CCGAGCATTC TGTCGGAAGA CCCAGGCAGG ATCGAGTGCC GCCCTCGCGG
    AATTCCATCA CCCTCACCAA CCTTAATCCG GGCACCGAGT ACGTTGTCAG CATCATTGCT
    GTTAATGGCA GAGAGGAGAG CCCGCCACTG ATTGGCCAGC AAGCCACAGT TTCTGATATT
    CCGAGAGATC TGGAGGTCAT TGCCTCCACC CCCACCAGCC TGCTCATCAG TTGGGAACCC
    CCTGCCGTCT CTGTGCGCTA TTACAGAATC ACCTACGGAG AGACAGGAGG AAATAGCCCT
    GTCCAGGAGT TCACTGTGCC CGGAAGCAAG TCCACAGCCA CCATCAACAA CATTAAACCA
    GGAGCAGACT ACACCATCAC CCTGTATGCT GTCACTGGCC GTGGGGACAG TCCAGCAAGC
    AGCAAGCCAG TTTCCATCAA TTATAAAACA GAAATTGACA AGCCGTCCCA GATGCAGGTG
    ACAGATGTCC AGGACAACAG CATCAGTGTC AGGTGGCTGC CTTCAACTTC TCCTGTGACA
    GGCTACAGAG TGACCACCAC TCCCAAAAAT GGCCTAGGAC CATCAAAAAC TAAAACTGCC
    AGTCCAGATC AAACAGAAAT GACCATTGAA GGTTTGCAAC CCACTGTGGA GTACGTGGTT
    AGTGTTTATG CTCAGAACCG GAACGGAGAA AGCCAGCCCC TGGTTCAAAC TGCAGTGACC
    ACCATTCCTG CGCCCACCAA TCTGAAGTTC AGTCAGGTGA CACCCACCAG CTTTACTGCC
    CAGTGGATAG CACCCAGTGT TCAGCTCACT GGCTACCGGG TGCGGGTGAA CCCGAAAGAG
    AAGACAGGAC CAATGAAAGA AATCAACCTT TCTCCAGACA GCTCATCGGT GATTGTGTCA
    GGACTCATGG TGGCCACTAA ATACGAAGTC AGTGTCTATG CTCTCAAGGA CACACTGACA
    AGCAGACCAG CCCAGGGAGT CATCACTACT CTGGAGAATG TTAGCCCTCC AAGAAGGGCC
    CGTGTGACGG ACGCTACAGA GACCACCATC ACTATTAGCT GGAGAACAAA GACAGAGACA
    ATCACTGGCT TCCAAGTCGA TGCCATCCCA GCCAATGGCC AGACCCCAGT TCAGAGGAGC
    ATCAGCCCGG ATGTTAGAAG CTACACCATT ACAGGTTTAC AGCCAGGCAC TGACTACAAG
    ATCCACCTGT ACACTCTGAA CGACAATGCC CGGAGCTCGC CCGTGATCAT CGATGCCTCC
    ACCGCCATCG ACGCACCATC CAACCTGCGG TTCCTGACCA CCACACCCAA CTCCTTGCTG
    GTGTCATGGC AGGCGCCCCG TGCCAGGATT ACTGGCTACA TTATCAAGTA TGAGAAGCCT
    GGATCCCCTC CCAGAGAAGT GGTCCCTCGG CCCCGCCCTG GTGTCACGGA GGCCACCATT
    ACTGGTCTGG AGCCAGGAAC CGAGTACACC ATCTACGTCA TTGCCCTGAA GAACAATCAG
    AAGAGTGAGC CCCTGATTGG GAGGAAGAAG ACAGGACAGG AAGCTCTCTC TCAGACAACC
    ATCTCTTGGA CGCCGTTCCA GGAGAGTTCT GAGTACATCA TTTCATGCCA ACCAGTTGGC
    ACCGACGAAG AGCCCTTACA GTTCCAAGTT CCTGGAACTT CTACCAGTGC GACTCTGACT
    GGCCTTACCA GAGGGGTCAC CTACAACATC ATAGTGGAGG CACTGCAGAA CCAGAGGAGG
    CACAAGGTTC GGGAAGAGGT TGTGACTGTG GGCAACGCTG TCAGCGAAGG CCTGAACCAG
    CCTACAGATG ACTCATGCTT TGACCCTTAC ACGGTTTCCC ATTACGCCAT TGGAGAGGAG
    TGGGAGCGGT TGTCTGACGC TGGCTTTAAG CTCACATGCC AGTGCTTGGG CTTTGGCAGT
    GGTCATTTCA GATGCGATTC ATCTAAATGG TGCCATGACA ACGGTGTCAA CTACAAGATC
    GGAGAGAAGT GGGATCGGCA GGGAGAAAAT GGCCAGCGGA TGAGCTGCAC ATGCCTCGGG
    AATGGAAAGG GAGAATTCAA GTGTGATCCC CATGAAGCAA CGTGCTATGA CGATGGGAAG
    ACCTACCATG TAGGAGAACA GTGGCAGAAA GAATATCTCG GAGCCATTTG CTCCTGCACG
    TGTTTCGGAG GCCAGCGGGG CTGGCGCTGT GACAACTGCC GTAGACCTGG GGCTGCTGAA
    CCCAGTCCCG ATGGCACCAC CGGCCACACC TACAACCAGT ATACACAGAG ATACAATCAG
    AGAACAAACA CTAACGTAAA TTGCCCCATT GAGTGCTTCA TGCCGCTAGA TGTGCAAGCT
    GACAGAGACG ATTCTCGAGA GTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu76977
    Clone ID Related Accession (Same CDS sequence) XM_017315837.2 , XM_017315837.1
    Accession Version XM_017315837.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6804bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-21
    Organism Mus musculus(house mouse)
    Product fibronectin isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    ATGCTCAGGG GTCCGGGACC CGGGCGGCTG CTGCTGCTGG CAGTCCTGTG CCTGGGGACC 
    TCGGTGCGCT GCACCGAAGC CGGGAAGAGC AAGAGGCAGG CTCAGCAAAT CGTGCAGCCT
    CAATCCCCGG TGGCTGTCAG TCAGAGCAAG CCTGGCTGTT TTGACAATGG GAAGCACTAT
    CAGATAAATC AGCAGTGGGA ACGGACCTAC CTAGGCAACG CCCTGGTTTG TACCTGCTAT
    GGAGGAAGCC GGGGTTTTAA CTGCGAGAGC AAGCCTGAGC CTGAAGAGAC TTGCTTTGAC
    AAATACACTG GGAACACTTA CAAAGTGGGT GACACTTATG AGCGCCCTAA AGATTCCATG
    ATCTGGGACT GTACCTGCAT CGGGGCTGGG AGAGGCAGGA TCAGCTGTAC CATTGCAAAT
    CGCTGCCATG AAGGGGGTCA GTCCTACAAG ATTGGCGACA AGTGGAGGAG GCCACATGAG
    ACTGGTGGCT ACATGTTAGA GTGTCTGTGT CTGGGAAATG GAAAAGGGGA ATGGACCTGC
    AAACCTATAG CTGAGAAGTG TTTTGATCAT GCTGCTGGGA CGTCCTACGT CGTGGGGGAG
    ACCTGGGAAA AGCCCTACCA AGGCTGGATG ATGGTGGACT GTACTTGTCT AGGCGAAGGC
    AATGGACGCA TCACCTGTAC CTCCAGAAAC AGATGCAACG ATCAGGACAC CCGGACATCC
    TATAGGATTG GAGACACGTG GAGCAAGAAG GACAACCGAG GAAACCTGCT TCAGTGTGTC
    TGCACAGGCA ATGGCAGAGG GGAGTGGAAG TGTGAGCGAC ATGCTCTACA AAGTGCTTCA
    GCCGGATCTG GCTCCTTCAC TGATGTCCGA ACAGCTATTT ACCAACCGCA GACTCACCCC
    CAGCCCGCTC CCTACGGCCA CTGTGTCACC GACAGTGGTG TGGTCTACTC TGTGGGAATG
    CAGTGGCTGA AGTCGCAAGG AAACAAGCAA ATGCTGTGCA CGTGCCTGGG CAATGGCGTC
    AGCTGCCAGG AGACAGCCGT GACCCAGACT TATGGTGGCA ATTCAAACGG GGAGCCCTGT
    GTCCTCCCGT TCACCTACAA CGGTAGGACC TTCTATTCCT GCACCACCGA AGGGCGGCAA
    GACGGACATC TGTGGTGTAG CACAACTTCC AATTACGAAC AAGACCAGAA GTATTCCTTC
    TGCACAGACC ATGCGGTTTT GGTTCAGACT CGAGGCGGAA ATTCCAATGG TGCTCTGTGC
    CACTTCCCCT TCCTGTACAA CAACCGGAAT TACACCGACT GTACTTCTGA GGGTCGCAGG
    GACAACATGA AATGGTGCGG CACCACCCAG AACTACGATG CCGATCAGAA GTTTGGATTC
    TGCCCAATGG CTGCCCACGA GGAGATCTGC ACAACCAATG AAGGGGTCAT GTATCGCATT
    GGGGATCAGT GGGATAAGCA GCATGACCTG GGCCACATGA TGAGGTGCAC GTGTGTGGGG
    AACGGTCGTG GAGAATGGGC CTGCATCCCC TACTCCCAGC TCCGAGACCA GTGCATCGTT
    GATGACATTA CTTACAATGT GAACGACACG TTCCACAAGC GTCACGAGGA GGGACATATG
    CTGAACTGTA CCTGCTTTGG TCAGGGCCGG GGCAGATGGA AGTGTGACCC CATTGACCAG
    TGCCAAGATT CAGAGACCCG GACATTTTAC CAGATTGGTG ACTCCTGGGA GAAGTTTGTG
    CATGGTGTCC GATACCAGTG TTACTGCTAC GGCCGTGGCA TCGGGGAGTG GCACTGTCAA
    CCTCTGCAGA CCTACCCAGG CACAACTGGA CCTGTCCAAG TAATTATCAC GGAGACCCCC
    AGCCAGCCCA ATTCCCACCC CATCCAGTGG AATGCCCCGG AGCCTTCACA CATCACCAAG
    TACATTCTCA GATGGAGACC TAAAACCTCT ACGGGTCGCT GGAAGGAAGC TACCATTCCT
    GGCCACCTTA ACTCCTATAC CATCAAAGGC CTGACCCCAG GTGTGATCTA TGAGGGACAG
    CTCATCAGCA TCCAGCAGTA TGGCCACAGA GAAGTGACAC GCTTCGACTT CACCACCAGC
    GCCAGCACCC CTGTGACCAG CAACACGGTG ACCGGAGAGA CTGCGCCCTA CTCTCCTGTT
    GTGGCCACTT CTGAATCTGT AACTGAAATC ACAGCCAGCA GCTTTGTGGT CTCATGGGTC
    TCAGCCTCCG ACACCGTGTC AGGCTTCCGG GTGGAGTATG AGCTGAGCGA GGAGGGAGAT
    GAACCACAGT ACCTTGATCT CCCAAGCACG GCCACTTCCG TGAACATTCC TGACCTGCTC
    CCGGGCAGAA AGTACATTGT CAATGTCTAT CAGATATCTG AGGAGGGAAA ACAGAGCTTG
    ATCCTGTCTA CCTCACAGAC TACAGCACCT GACGCTCCTC CAGACCCTAC CGTGGACCAG
    GTTGATGATA CTTCCATTGT TGTTCGGTGG AGTAGACCCC AGGCACCTAT CACAGGGTAT
    AGAATTGTCT ATTCACCTTC AGTAGAAGGC AGTAGCACAG AGCTCAACCT CCCTGAAACG
    GCCAACTCCG TCACCCTCAG CGACCTGCAG CCCGGTGTTC AGTACAACAT CACTATCTAT
    GCTGTGGAGG AGAACCAGGA GAGCACACCC GTTTTCATCC AACAAGAGAC CACTGGCACC
    CCAAGATCTG ATAACGTCCC CCCTCCGACG GACCTACAGT TTGTGGAACT GACTGATGTG
    AAAGTCACCA TCATGTGGAC CCCTCCTGAT AGTGTGGTGT CTGGATACCG TGTGGAGGTC
    CTGCCTGTCA GCCTGCCCGG GGAACATGGG CAGAGGCTGC CTGTCAACAG AAATACCTTT
    GCTGAAATCA CTGGGCTGTC CCCTGGGGTC ACGTACCTCT TCAAAGTCTT TGCTGTGCAC
    CAGGGCAGGG AAAGCAATCC TCTGACGGCA CAACAGACCA CCAAACTCGA CGCTCCCACT
    AACCTCCAGT TTGTCAATGA AACTGACAGA ACAGTTCTGG TAACGTGGAC TCCACCTCGA
    GCCCGTATAG CAGGCTACCG ACTGACCGCG GGCCTGACCC GAGGAGGCCA GCCCAAGCAG
    TACAATGTGG GACCCTTGGC CTCCAAGTAT CCCCTGAGAA ATCTGCAGCC TGGGTCTGAG
    TACACCGTGA CCTTGGTGGC TGTGAAAGGG AACCAGCAGA GTCCCAAAGC CACCGGAGTC
    TTTACTACCC TGCAGCCTCT GCGCTCCATT CCACCTTACA ACACCGAGGT GACAGAGACC
    ACAATTGTGA TCACCTGGAC CCCCGCTCCA AGGATTGGCT TCAAGCTGGG TGTACGACCG
    AGCCAGGGAG GTGAGGCACC CCGAGAAGTG ACTTCAGACT CTGGGAGCAT TGTTGTGTCT
    GGCTTGACTC CAGGCGTGGA ATACACTTAC ACCATCCAAG TCCTGCGAGA TGGCCAGGAG
    AGAGATGCAC CGATTGTCAA CAGAGTAGTG ACACCGCTGT CTCCACCGAC CAACTTGCAT
    CTGGAGGCAA ACCCTGACAC TGGAGTGCTT ACTGTCTCCT GGGAGAGGAG CACTACCCCA
    GATATCACTG GCTACAGAAT AACTACTACC CCCACGAACG GGCAGCAGGG GACCTCTCTG
    GAAGAAGTGG TCCATGCTGA TCAGAGTTCC TGCACTTTTG AGAACCTGAA TCCTGGCCTG
    GAGTACAACG TCAGTGTTTA CACTGTCAAA GATGACAAGG AAAGTGCCCC TATCTCTGAT
    ACCGTTGTCC CAGAGGTGCC CCAGCTCACT GACCTAAGCT TTGTTGATAT AACTGATTCA
    AGCATCGGCC TGAGGTGGAC CCCGCTAAAC TCTTCCACCA TTATCGGGTA CCGAATCACA
    GTAGTTGCGG CAGGAGAAGG GATCCCTATT TTTGAAGATT TTGTGGACTC CTCAGTAGGA
    TACTACACAG TTACAGGGCT GGAGCCTGGC ATTGACTATG ACATCAGCGT TATCACTCTC
    ATTAATGGCG GAGAGAGTGC CCCTACTACA CTGACACAGC AAACGGCTGT CCCTCCTCCC
    ACGGATCTGC GATTCACCAA TATCGGTCCA GACACGATGC GGGTCACTTG GGCCCCGCCT
    CCGTCCATCG AGCTGACCAA CCTCTTGGTG CGCTACTCAC CCGTGAAGAA TGAAGAGGAC
    GTTGCAGAGC TATCCATTTC ACCTTCAGAC AATGCCGTGG TCCTAACAAA TCTCCTGCCT
    GGGACAGAAT ACTTAGTCAG TGTCTCCAGT GTCTACGAAC AACATGAGAG CATCCCTCTC
    CGGGGAAGGC AGAAAACAGG TCTCGATTCC CCAACTGGTT TTGATTCTTC TGATATCACC
    GCCAACTCAT TCACTGTCCA CTGGGTGGCT CCTCGGGCCC CCATCACCGG CTACATCATC
    CGCCATCACG CCGAGCATTC TGTCGGAAGA CCCAGGCAGG ATCGAGTGCC GCCCTCGCGG
    AATTCCATCA CCCTCACCAA CCTTAATCCG GGCACCGAGT ACGTTGTCAG CATCATTGCT
    GTTAATGGCA GAGAGGAGAG CCCGCCACTG ATTGGCCAGC AAGCCACAGT TTCTGATATT
    CCGAGAGATC TGGAGGTCAT TGCCTCCACC CCCACCAGCC TGCTCATCAG TTGGGAACCC
    CCTGCCGTCT CTGTGCGCTA TTACAGAATC ACCTACGGAG AGACAGGAGG AAATAGCCCT
    GTCCAGGAGT TCACTGTGCC CGGAAGCAAG TCCACAGCCA CCATCAACAA CATTAAACCA
    GGAGCAGACT ACACCATCAC CCTGTATGCT GTCACTGGCC GTGGGGACAG TCCAGCAAGC
    AGCAAGCCAG TTTCCATCAA TTATAAAACA GAAATTGACA AGCCGTCCCA GATGCAGGTG
    ACAGATGTCC AGGACAACAG CATCAGTGTC AGGTGGCTGC CTTCAACTTC TCCTGTGACA
    GGCTACAGAG TGACCACCAC TCCCAAAAAT GGCCTAGGAC CATCAAAAAC TAAAACTGCC
    AGTCCAGATC AAACAGAAAT GACCATTGAA GGTTTGCAAC CCACTGTGGA GTACGTGGTT
    AGTGTTTATG CTCAGAACCG GAACGGAGAA AGCCAGCCCC TGGTTCAAAC TGCAGTGACC
    ACCATTCCTG CGCCCACCAA TCTGAAGTTC AGTCAGGTGA CACCCACCAG CTTTACTGCC
    CAGTGGATAG CACCCAGTGT TCAGCTCACT GGCTACCGGG TGCGGGTGAA CCCGAAAGAG
    AAGACAGGAC CAATGAAAGA AATCAACCTT TCTCCAGACA GCTCATCGGT GATTGTGTCA
    GGACTCATGG TGGCCACTAA ATACGAAGTC AGTGTCTATG CTCTCAAGGA CACACTGACA
    AGCAGACCAG CCCAGGGAGT CATCACTACT CTGGAGAATG TTAGCCCTCC AAGAAGGGCC
    CGTGTGACGG ACGCTACAGA GACCACCATC ACTATTAGCT GGAGAACAAA GACAGAGACA
    ATCACTGGCT TCCAAGTCGA TGCCATCCCA GCCAATGGCC AGACCCCAGT TCAGAGGAGC
    ATCAGCCCGG ATGTTAGAAG CTACACCATT ACAGGTTTAC AGCCAGGCAC TGACTACAAG
    ATCCACCTGT ACACTCTGAA CGACAATGCC CGGAGCTCGC CCGTGATCAT CGATGCCTCC
    ACCGCCATCG ACGCACCATC CAACCTGCGG TTCCTGACCA CCACACCCAA CTCCTTGCTG
    GTGTCATGGC AGGCGCCCCG TGCCAGGATT ACTGGCTACA TTATCAAGTA TGAGAAGCCT
    GGATCCCCTC CCAGAGAAGT GGTCCCTCGG CCCCGCCCTG GTGTCACGGA GGCCACCATT
    ACTGGTCTGG AGCCAGGAAC CGAGTACACC ATCTACGTCA TTGCCCTGAA GAACAATCAG
    AAGAGTGAGC CCCTGATTGG GAGGAAGAAG ACAGGACAGG AAGCTCTCTC TCAGACAACC
    ATCTCTTGGA CGCCGTTCCA GGAGAGTTCT GAGTACATCA TTTCATGCCA ACCAGTTGGC
    ACCGACGAAG AGCCCTTACA GTTCCAAGTT CCTGGAACTT CTACCAGTGC GACTCTGACT
    GGCCTTACCA GAGGGGTCAC CTACAACATC ATAGTGGAGG CACTGCAGAA CCAGAGGAGG
    CACAAGGTTC GGGAAGAGGT TGTGACTGTG GGCAACGCTG TCAGCGAAGG CCTGAACCAG
    CCTACAGATG ACTCATGCTT TGACCCTTAC ACGGTTTCCC ATTACGCCAT TGGAGAGGAG
    TGGGAGCGGT TGTCTGACGC TGGCTTTAAG CTCACATGCC AGTGCTTGGG CTTTGGCAGT
    GGTCATTTCA GATGCGATTC ATCTAAATGG TGCCATGACA ACGGTGTCAA CTACAAGATC
    GGAGAGAAGT GGGATCGGCA GGGAGAAAAT GGCCAGCGGA TGAGCTGCAC ATGCCTCGGG
    AATGGAAAGG GAGAATTCAA GTGTGATCCC CATGAAGCAA CGTGCTATGA CGATGGGAAG
    ACCTACCATG TAGGAGAACA GTGGCAGAAA GAATATCTCG GAGCCATTTG CTCCTGCACG
    TGTTTCGGAG GCCAGCGGGG CTGGCGCTGT GACAACTGCC GTAGACCTGG GGCTGCTGAA
    CCCAGTCCCG ATGGCACCAC CGGCCACACC TACAACCAGT ATACACAGAG ATACAATCAG
    AGAACAAACA CTAACGTAAA TTGCCCCATT GAGTGCTTCA TGCCGCTAGA TGTGCAAGCT
    GACAGAGACG ATTCTCGAGA GTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017171326.1
    CDS248..7051
    Translation

    Target ORF information:

    RefSeq Version XM_017315837.1
    Organism Mus musculus(house mouse)
    Definition Mus musculus fibronectin 1 (Fn1), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017315837.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    ATGCTCAGGG GTCCGGGACC CGGGCGGCTG CTGCTGCTGG CAGTCCTGTG CCTGGGGACC 
    TCGGTGCGCT GCACCGAAGC CGGGAAGAGC AAGAGGCAGG CTCAGCAAAT CGTGCAGCCT
    CAATCCCCGG TGGCTGTCAG TCAGAGCAAG CCTGGCTGTT TTGACAATGG GAAGCACTAT
    CAGATAAATC AGCAGTGGGA ACGGACCTAC CTAGGCAACG CCCTGGTTTG TACCTGCTAT
    GGAGGAAGCC GGGGTTTTAA CTGCGAGAGC AAGCCTGAGC CTGAAGAGAC TTGCTTTGAC
    AAATACACTG GGAACACTTA CAAAGTGGGT GACACTTATG AGCGCCCTAA AGATTCCATG
    ATCTGGGACT GTACCTGCAT CGGGGCTGGG AGAGGCAGGA TCAGCTGTAC CATTGCAAAT
    CGCTGCCATG AAGGGGGTCA GTCCTACAAG ATTGGCGACA AGTGGAGGAG GCCACATGAG
    ACTGGTGGCT ACATGTTAGA GTGTCTGTGT CTGGGAAATG GAAAAGGGGA ATGGACCTGC
    AAACCTATAG CTGAGAAGTG TTTTGATCAT GCTGCTGGGA CGTCCTACGT CGTGGGGGAG
    ACCTGGGAAA AGCCCTACCA AGGCTGGATG ATGGTGGACT GTACTTGTCT AGGCGAAGGC
    AATGGACGCA TCACCTGTAC CTCCAGAAAC AGATGCAACG ATCAGGACAC CCGGACATCC
    TATAGGATTG GAGACACGTG GAGCAAGAAG GACAACCGAG GAAACCTGCT TCAGTGTGTC
    TGCACAGGCA ATGGCAGAGG GGAGTGGAAG TGTGAGCGAC ATGCTCTACA AAGTGCTTCA
    GCCGGATCTG GCTCCTTCAC TGATGTCCGA ACAGCTATTT ACCAACCGCA GACTCACCCC
    CAGCCCGCTC CCTACGGCCA CTGTGTCACC GACAGTGGTG TGGTCTACTC TGTGGGAATG
    CAGTGGCTGA AGTCGCAAGG AAACAAGCAA ATGCTGTGCA CGTGCCTGGG CAATGGCGTC
    AGCTGCCAGG AGACAGCCGT GACCCAGACT TATGGTGGCA ATTCAAACGG GGAGCCCTGT
    GTCCTCCCGT TCACCTACAA CGGTAGGACC TTCTATTCCT GCACCACCGA AGGGCGGCAA
    GACGGACATC TGTGGTGTAG CACAACTTCC AATTACGAAC AAGACCAGAA GTATTCCTTC
    TGCACAGACC ATGCGGTTTT GGTTCAGACT CGAGGCGGAA ATTCCAATGG TGCTCTGTGC
    CACTTCCCCT TCCTGTACAA CAACCGGAAT TACACCGACT GTACTTCTGA GGGTCGCAGG
    GACAACATGA AATGGTGCGG CACCACCCAG AACTACGATG CCGATCAGAA GTTTGGATTC
    TGCCCAATGG CTGCCCACGA GGAGATCTGC ACAACCAATG AAGGGGTCAT GTATCGCATT
    GGGGATCAGT GGGATAAGCA GCATGACCTG GGCCACATGA TGAGGTGCAC GTGTGTGGGG
    AACGGTCGTG GAGAATGGGC CTGCATCCCC TACTCCCAGC TCCGAGACCA GTGCATCGTT
    GATGACATTA CTTACAATGT GAACGACACG TTCCACAAGC GTCACGAGGA GGGACATATG
    CTGAACTGTA CCTGCTTTGG TCAGGGCCGG GGCAGATGGA AGTGTGACCC CATTGACCAG
    TGCCAAGATT CAGAGACCCG GACATTTTAC CAGATTGGTG ACTCCTGGGA GAAGTTTGTG
    CATGGTGTCC GATACCAGTG TTACTGCTAC GGCCGTGGCA TCGGGGAGTG GCACTGTCAA
    CCTCTGCAGA CCTACCCAGG CACAACTGGA CCTGTCCAAG TAATTATCAC GGAGACCCCC
    AGCCAGCCCA ATTCCCACCC CATCCAGTGG AATGCCCCGG AGCCTTCACA CATCACCAAG
    TACATTCTCA GATGGAGACC TAAAACCTCT ACGGGTCGCT GGAAGGAAGC TACCATTCCT
    GGCCACCTTA ACTCCTATAC CATCAAAGGC CTGACCCCAG GTGTGATCTA TGAGGGACAG
    CTCATCAGCA TCCAGCAGTA TGGCCACAGA GAAGTGACAC GCTTCGACTT CACCACCAGC
    GCCAGCACCC CTGTGACCAG CAACACGGTG ACCGGAGAGA CTGCGCCCTA CTCTCCTGTT
    GTGGCCACTT CTGAATCTGT AACTGAAATC ACAGCCAGCA GCTTTGTGGT CTCATGGGTC
    TCAGCCTCCG ACACCGTGTC AGGCTTCCGG GTGGAGTATG AGCTGAGCGA GGAGGGAGAT
    GAACCACAGT ACCTTGATCT CCCAAGCACG GCCACTTCCG TGAACATTCC TGACCTGCTC
    CCGGGCAGAA AGTACATTGT CAATGTCTAT CAGATATCTG AGGAGGGAAA ACAGAGCTTG
    ATCCTGTCTA CCTCACAGAC TACAGCACCT GACGCTCCTC CAGACCCTAC CGTGGACCAG
    GTTGATGATA CTTCCATTGT TGTTCGGTGG AGTAGACCCC AGGCACCTAT CACAGGGTAT
    AGAATTGTCT ATTCACCTTC AGTAGAAGGC AGTAGCACAG AGCTCAACCT CCCTGAAACG
    GCCAACTCCG TCACCCTCAG CGACCTGCAG CCCGGTGTTC AGTACAACAT CACTATCTAT
    GCTGTGGAGG AGAACCAGGA GAGCACACCC GTTTTCATCC AACAAGAGAC CACTGGCACC
    CCAAGATCTG ATAACGTCCC CCCTCCGACG GACCTACAGT TTGTGGAACT GACTGATGTG
    AAAGTCACCA TCATGTGGAC CCCTCCTGAT AGTGTGGTGT CTGGATACCG TGTGGAGGTC
    CTGCCTGTCA GCCTGCCCGG GGAACATGGG CAGAGGCTGC CTGTCAACAG AAATACCTTT
    GCTGAAATCA CTGGGCTGTC CCCTGGGGTC ACGTACCTCT TCAAAGTCTT TGCTGTGCAC
    CAGGGCAGGG AAAGCAATCC TCTGACGGCA CAACAGACCA CCAAACTCGA CGCTCCCACT
    AACCTCCAGT TTGTCAATGA AACTGACAGA ACAGTTCTGG TAACGTGGAC TCCACCTCGA
    GCCCGTATAG CAGGCTACCG ACTGACCGCG GGCCTGACCC GAGGAGGCCA GCCCAAGCAG
    TACAATGTGG GACCCTTGGC CTCCAAGTAT CCCCTGAGAA ATCTGCAGCC TGGGTCTGAG
    TACACCGTGA CCTTGGTGGC TGTGAAAGGG AACCAGCAGA GTCCCAAAGC CACCGGAGTC
    TTTACTACCC TGCAGCCTCT GCGCTCCATT CCACCTTACA ACACCGAGGT GACAGAGACC
    ACAATTGTGA TCACCTGGAC CCCCGCTCCA AGGATTGGCT TCAAGCTGGG TGTACGACCG
    AGCCAGGGAG GTGAGGCACC CCGAGAAGTG ACTTCAGACT CTGGGAGCAT TGTTGTGTCT
    GGCTTGACTC CAGGCGTGGA ATACACTTAC ACCATCCAAG TCCTGCGAGA TGGCCAGGAG
    AGAGATGCAC CGATTGTCAA CAGAGTAGTG ACACCGCTGT CTCCACCGAC CAACTTGCAT
    CTGGAGGCAA ACCCTGACAC TGGAGTGCTT ACTGTCTCCT GGGAGAGGAG CACTACCCCA
    GATATCACTG GCTACAGAAT AACTACTACC CCCACGAACG GGCAGCAGGG GACCTCTCTG
    GAAGAAGTGG TCCATGCTGA TCAGAGTTCC TGCACTTTTG AGAACCTGAA TCCTGGCCTG
    GAGTACAACG TCAGTGTTTA CACTGTCAAA GATGACAAGG AAAGTGCCCC TATCTCTGAT
    ACCGTTGTCC CAGAGGTGCC CCAGCTCACT GACCTAAGCT TTGTTGATAT AACTGATTCA
    AGCATCGGCC TGAGGTGGAC CCCGCTAAAC TCTTCCACCA TTATCGGGTA CCGAATCACA
    GTAGTTGCGG CAGGAGAAGG GATCCCTATT TTTGAAGATT TTGTGGACTC CTCAGTAGGA
    TACTACACAG TTACAGGGCT GGAGCCTGGC ATTGACTATG ACATCAGCGT TATCACTCTC
    ATTAATGGCG GAGAGAGTGC CCCTACTACA CTGACACAGC AAACGGCTGT CCCTCCTCCC
    ACGGATCTGC GATTCACCAA TATCGGTCCA GACACGATGC GGGTCACTTG GGCCCCGCCT
    CCGTCCATCG AGCTGACCAA CCTCTTGGTG CGCTACTCAC CCGTGAAGAA TGAAGAGGAC
    GTTGCAGAGC TATCCATTTC ACCTTCAGAC AATGCCGTGG TCCTAACAAA TCTCCTGCCT
    GGGACAGAAT ACTTAGTCAG TGTCTCCAGT GTCTACGAAC AACATGAGAG CATCCCTCTC
    CGGGGAAGGC AGAAAACAGG TCTCGATTCC CCAACTGGTT TTGATTCTTC TGATATCACC
    GCCAACTCAT TCACTGTCCA CTGGGTGGCT CCTCGGGCCC CCATCACCGG CTACATCATC
    CGCCATCACG CCGAGCATTC TGTCGGAAGA CCCAGGCAGG ATCGAGTGCC GCCCTCGCGG
    AATTCCATCA CCCTCACCAA CCTTAATCCG GGCACCGAGT ACGTTGTCAG CATCATTGCT
    GTTAATGGCA GAGAGGAGAG CCCGCCACTG ATTGGCCAGC AAGCCACAGT TTCTGATATT
    CCGAGAGATC TGGAGGTCAT TGCCTCCACC CCCACCAGCC TGCTCATCAG TTGGGAACCC
    CCTGCCGTCT CTGTGCGCTA TTACAGAATC ACCTACGGAG AGACAGGAGG AAATAGCCCT
    GTCCAGGAGT TCACTGTGCC CGGAAGCAAG TCCACAGCCA CCATCAACAA CATTAAACCA
    GGAGCAGACT ACACCATCAC CCTGTATGCT GTCACTGGCC GTGGGGACAG TCCAGCAAGC
    AGCAAGCCAG TTTCCATCAA TTATAAAACA GAAATTGACA AGCCGTCCCA GATGCAGGTG
    ACAGATGTCC AGGACAACAG CATCAGTGTC AGGTGGCTGC CTTCAACTTC TCCTGTGACA
    GGCTACAGAG TGACCACCAC TCCCAAAAAT GGCCTAGGAC CATCAAAAAC TAAAACTGCC
    AGTCCAGATC AAACAGAAAT GACCATTGAA GGTTTGCAAC CCACTGTGGA GTACGTGGTT
    AGTGTTTATG CTCAGAACCG GAACGGAGAA AGCCAGCCCC TGGTTCAAAC TGCAGTGACC
    ACCATTCCTG CGCCCACCAA TCTGAAGTTC AGTCAGGTGA CACCCACCAG CTTTACTGCC
    CAGTGGATAG CACCCAGTGT TCAGCTCACT GGCTACCGGG TGCGGGTGAA CCCGAAAGAG
    AAGACAGGAC CAATGAAAGA AATCAACCTT TCTCCAGACA GCTCATCGGT GATTGTGTCA
    GGACTCATGG TGGCCACTAA ATACGAAGTC AGTGTCTATG CTCTCAAGGA CACACTGACA
    AGCAGACCAG CCCAGGGAGT CATCACTACT CTGGAGAATG TTAGCCCTCC AAGAAGGGCC
    CGTGTGACGG ACGCTACAGA GACCACCATC ACTATTAGCT GGAGAACAAA GACAGAGACA
    ATCACTGGCT TCCAAGTCGA TGCCATCCCA GCCAATGGCC AGACCCCAGT TCAGAGGAGC
    ATCAGCCCGG ATGTTAGAAG CTACACCATT ACAGGTTTAC AGCCAGGCAC TGACTACAAG
    ATCCACCTGT ACACTCTGAA CGACAATGCC CGGAGCTCGC CCGTGATCAT CGATGCCTCC
    ACCGCCATCG ACGCACCATC CAACCTGCGG TTCCTGACCA CCACACCCAA CTCCTTGCTG
    GTGTCATGGC AGGCGCCCCG TGCCAGGATT ACTGGCTACA TTATCAAGTA TGAGAAGCCT
    GGATCCCCTC CCAGAGAAGT GGTCCCTCGG CCCCGCCCTG GTGTCACGGA GGCCACCATT
    ACTGGTCTGG AGCCAGGAAC CGAGTACACC ATCTACGTCA TTGCCCTGAA GAACAATCAG
    AAGAGTGAGC CCCTGATTGG GAGGAAGAAG ACAGGACAGG AAGCTCTCTC TCAGACAACC
    ATCTCTTGGA CGCCGTTCCA GGAGAGTTCT GAGTACATCA TTTCATGCCA ACCAGTTGGC
    ACCGACGAAG AGCCCTTACA GTTCCAAGTT CCTGGAACTT CTACCAGTGC GACTCTGACT
    GGCCTTACCA GAGGGGTCAC CTACAACATC ATAGTGGAGG CACTGCAGAA CCAGAGGAGG
    CACAAGGTTC GGGAAGAGGT TGTGACTGTG GGCAACGCTG TCAGCGAAGG CCTGAACCAG
    CCTACAGATG ACTCATGCTT TGACCCTTAC ACGGTTTCCC ATTACGCCAT TGGAGAGGAG
    TGGGAGCGGT TGTCTGACGC TGGCTTTAAG CTCACATGCC AGTGCTTGGG CTTTGGCAGT
    GGTCATTTCA GATGCGATTC ATCTAAATGG TGCCATGACA ACGGTGTCAA CTACAAGATC
    GGAGAGAAGT GGGATCGGCA GGGAGAAAAT GGCCAGCGGA TGAGCTGCAC ATGCCTCGGG
    AATGGAAAGG GAGAATTCAA GTGTGATCCC CATGAAGCAA CGTGCTATGA CGATGGGAAG
    ACCTACCATG TAGGAGAACA GTGGCAGAAA GAATATCTCG GAGCCATTTG CTCCTGCACG
    TGTTTCGGAG GCCAGCGGGG CTGGCGCTGT GACAACTGCC GTAGACCTGG GGCTGCTGAA
    CCCAGTCCCG ATGGCACCAC CGGCCACACC TACAACCAGT ATACACAGAG ATACAATCAG
    AGAACAAACA CTAACGTAAA TTGCCCCATT GAGTGCTTCA TGCCGCTAGA TGTGCAAGCT
    GACAGAGACG ATTCTCGAGA GTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu76978
    Clone ID Related Accession (Same CDS sequence) XM_017315848.2 , XM_017315848.1
    Accession Version XM_017315848.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6726bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product fibronectin isoform X8
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_017315848.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    ATGCTCAGGG GTCCGGGACC CGGGCGGCTG CTGCTGCTGG CAGTCCTGTG CCTGGGGACC 
    TCGGTGCGCT GCACCGAAGC CGGGAAGAGC AAGAGGCAGG CTCAGCAAAT CGTGCAGCCT
    CAATCCCCGG TGGCTGTCAG TCAGAGCAAG CCTGGCTGTT TTGACAATGG GAAGCACTAT
    CAGATAAATC AGCAGTGGGA ACGGACCTAC CTAGGCAACG CCCTGGTTTG TACCTGCTAT
    GGAGGAAGCC GGGGTTTTAA CTGCGAGAGC AAGCCTGAGC CTGAAGAGAC TTGCTTTGAC
    AAATACACTG GGAACACTTA CAAAGTGGGT GACACTTATG AGCGCCCTAA AGATTCCATG
    ATCTGGGACT GTACCTGCAT CGGGGCTGGG AGAGGCAGGA TCAGCTGTAC CATTGCAAAT
    CGCTGCCATG AAGGGGGTCA GTCCTACAAG ATTGGCGACA AGTGGAGGAG GCCACATGAG
    ACTGGTGGCT ACATGTTAGA GTGTCTGTGT CTGGGAAATG GAAAAGGGGA ATGGACCTGC
    AAACCTATAG CTGAGAAGTG TTTTGATCAT GCTGCTGGGA CGTCCTACGT CGTGGGGGAG
    ACCTGGGAAA AGCCCTACCA AGGCTGGATG ATGGTGGACT GTACTTGTCT AGGCGAAGGC
    AATGGACGCA TCACCTGTAC CTCCAGAAAC AGATGCAACG ATCAGGACAC CCGGACATCC
    TATAGGATTG GAGACACGTG GAGCAAGAAG GACAACCGAG GAAACCTGCT TCAGTGTGTC
    TGCACAGGCA ATGGCAGAGG GGAGTGGAAG TGTGAGCGAC ATGCTCTACA AAGTGCTTCA
    GCCGGATCTG GCTCCTTCAC TGATGTCCGA ACAGCTATTT ACCAACCGCA GACTCACCCC
    CAGCCCGCTC CCTACGGCCA CTGTGTCACC GACAGTGGTG TGGTCTACTC TGTGGGAATG
    CAGTGGCTGA AGTCGCAAGG AAACAAGCAA ATGCTGTGCA CGTGCCTGGG CAATGGCGTC
    AGCTGCCAGG AGACAGCCGT GACCCAGACT TATGGTGGCA ATTCAAACGG GGAGCCCTGT
    GTCCTCCCGT TCACCTACAA CGGTAGGACC TTCTATTCCT GCACCACCGA AGGGCGGCAA
    GACGGACATC TGTGGTGTAG CACAACTTCC AATTACGAAC AAGACCAGAA GTATTCCTTC
    TGCACAGACC ATGCGGTTTT GGTTCAGACT CGAGGCGGAA ATTCCAATGG TGCTCTGTGC
    CACTTCCCCT TCCTGTACAA CAACCGGAAT TACACCGACT GTACTTCTGA GGGTCGCAGG
    GACAACATGA AATGGTGCGG CACCACCCAG AACTACGATG CCGATCAGAA GTTTGGATTC
    TGCCCAATGG CTGCCCACGA GGAGATCTGC ACAACCAATG AAGGGGTCAT GTATCGCATT
    GGGGATCAGT GGGATAAGCA GCATGACCTG GGCCACATGA TGAGGTGCAC GTGTGTGGGG
    AACGGTCGTG GAGAATGGGC CTGCATCCCC TACTCCCAGC TCCGAGACCA GTGCATCGTT
    GATGACATTA CTTACAATGT GAACGACACG TTCCACAAGC GTCACGAGGA GGGACATATG
    CTGAACTGTA CCTGCTTTGG TCAGGGCCGG GGCAGATGGA AGTGTGACCC CATTGACCAG
    TGCCAAGATT CAGAGACCCG GACATTTTAC CAGATTGGTG ACTCCTGGGA GAAGTTTGTG
    CATGGTGTCC GATACCAGTG TTACTGCTAC GGCCGTGGCA TCGGGGAGTG GCACTGTCAA
    CCTCTGCAGA CCTACCCAGG CACAACTGGA CCTGTCCAAG TAATTATCAC GGAGACCCCC
    AGCCAGCCCA ATTCCCACCC CATCCAGTGG AATGCCCCGG AGCCTTCACA CATCACCAAG
    TACATTCTCA GATGGAGACC TAAAACCTCT ACGGGTCGCT GGAAGGAAGC TACCATTCCT
    GGCCACCTTA ACTCCTATAC CATCAAAGGC CTGACCCCAG GTGTGATCTA TGAGGGACAG
    CTCATCAGCA TCCAGCAGTA TGGCCACAGA GAAGTGACAC GCTTCGACTT CACCACCAGC
    GCCAGCACCC CTGTGACCAG CAACACGGTG ACCGGAGAGA CTGCGCCCTA CTCTCCTGTT
    GTGGCCACTT CTGAATCTGT AACTGAAATC ACAGCCAGCA GCTTTGTGGT CTCATGGGTC
    TCAGCCTCCG ACACCGTGTC AGGCTTCCGG GTGGAGTATG AGCTGAGCGA GGAGGGAGAT
    GAACCACAGT ACCTTGATCT CCCAAGCACG GCCACTTCCG TGAACATTCC TGACCTGCTC
    CCGGGCAGAA AGTACATTGT CAATGTCTAT CAGATATCTG AGGAGGGAAA ACAGAGCTTG
    ATCCTGTCTA CCTCACAGAC TACAGCACCT GACGCTCCTC CAGACCCTAC CGTGGACCAG
    GTTGATGATA CTTCCATTGT TGTTCGGTGG AGTAGACCCC AGGCACCTAT CACAGGGTAT
    AGAATTGTCT ATTCACCTTC AGTAGAAGGC AGTAGCACAG AGCTCAACCT CCCTGAAACG
    GCCAACTCCG TCACCCTCAG CGACCTGCAG CCCGGTGTTC AGTACAACAT CACTATCTAT
    GCTGTGGAGG AGAACCAGGA GAGCACACCC GTTTTCATCC AACAAGAGAC CACTGGCACC
    CCAAGATCTG ATAACGTCCC CCCTCCGACG GACCTACAGT TTGTGGAACT GACTGATGTG
    AAAGTCACCA TCATGTGGAC CCCTCCTGAT AGTGTGGTGT CTGGATACCG TGTGGAGGTC
    CTGCCTGTCA GCCTGCCCGG GGAACATGGG CAGAGGCTGC CTGTCAACAG AAATACCTTT
    GCTGAAATCA CTGGGCTGTC CCCTGGGGTC ACGTACCTCT TCAAAGTCTT TGCTGTGCAC
    CAGGGCAGGG AAAGCAATCC TCTGACGGCA CAACAGACCA CCAAACTCGA CGCTCCCACT
    AACCTCCAGT TTGTCAATGA AACTGACAGA ACAGTTCTGG TAACGTGGAC TCCACCTCGA
    GCCCGTATAG CAGGCTACCG ACTGACCGCG GGCCTGACCC GAGGAGGCCA GCCCAAGCAG
    TACAATGTGG GACCCTTGGC CTCCAAGTAT CCCCTGAGAA ATCTGCAGCC TGGGTCTGAG
    TACACCGTGA CCTTGGTGGC TGTGAAAGGG AACCAGCAGA GTCCCAAAGC CACCGGAGTC
    TTTACTACCC TGCAGCCTCT GCGCTCCATT CCACCTTACA ACACCGAGGT GACAGAGACC
    ACAATTGTGA TCACCTGGAC CCCCGCTCCA AGGATTGGCT TCAAGCTGGG TGTACGACCG
    AGCCAGGGAG GTGAGGCACC CCGAGAAGTG ACTTCAGACT CTGGGAGCAT TGTTGTGTCT
    GGCTTGACTC CAGGCGTGGA ATACACTTAC ACCATCCAAG TCCTGCGAGA TGGCCAGGAG
    AGAGATGCAC CGATTGTCAA CAGAGTAGTG ACACCGCTGT CTCCACCGAC CAACTTGCAT
    CTGGAGGCAA ACCCTGACAC TGGAGTGCTT ACTGTCTCCT GGGAGAGGAG CACTACCCCA
    GATATCACTG GCTACAGAAT AACTACTACC CCCACGAACG GGCAGCAGGG GACCTCTCTG
    GAAGAAGTGG TCCATGCTGA TCAGAGTTCC TGCACTTTTG AGAACCTGAA TCCTGGCCTG
    GAGTACAACG TCAGTGTTTA CACTGTCAAA GATGACAAGG AAAGTGCCCC TATCTCTGAT
    ACCGTTGTCC CAGCTGTCCC TCCTCCCACG GATCTGCGAT TCACCAATAT CGGTCCAGAC
    ACGATGCGGG TCACTTGGGC CCCGCCTCCG TCCATCGAGC TGACCAACCT CTTGGTGCGC
    TACTCACCCG TGAAGAATGA AGAGGACGTT GCAGAGCTAT CCATTTCACC TTCAGACAAT
    GCCGTGGTCC TAACAAATCT CCTGCCTGGG ACAGAATACT TAGTCAGTGT CTCCAGTGTC
    TACGAACAAC ATGAGAGCAT CCCTCTCCGG GGAAGGCAGA AAACAGGTCT CGATTCCCCA
    ACTGGTTTTG ATTCTTCTGA TATCACCGCC AACTCATTCA CTGTCCACTG GGTGGCTCCT
    CGGGCCCCCA TCACCGGCTA CATCATCCGC CATCACGCCG AGCATTCTGT CGGAAGACCC
    AGGCAGGATC GAGTGCCGCC CTCGCGGAAT TCCATCACCC TCACCAACCT TAATCCGGGC
    ACCGAGTACG TTGTCAGCAT CATTGCTGTT AATGGCAGAG AGGAGAGCCC GCCACTGATT
    GGCCAGCAAG CCACAGTTTC TGATATTCCG AGAGATCTGG AGGTCATTGC CTCCACCCCC
    ACCAGCCTGC TCATCAGTTG GGAACCCCCT GCCGTCTCTG TGCGCTATTA CAGAATCACC
    TACGGAGAGA CAGGAGGAAA TAGCCCTGTC CAGGAGTTCA CTGTGCCCGG AAGCAAGTCC
    ACAGCCACCA TCAACAACAT TAAACCAGGA GCAGACTACA CCATCACCCT GTATGCTGTC
    ACTGGCCGTG GGGACAGTCC AGCAAGCAGC AAGCCAGTTT CCATCAATTA TAAAACAGAA
    ATTGACAAGC CGTCCCAGAT GCAGGTGACA GATGTCCAGG ACAACAGCAT CAGTGTCAGG
    TGGCTGCCTT CAACTTCTCC TGTGACAGGC TACAGAGTGA CCACCACTCC CAAAAATGGC
    CTAGGACCAT CAAAAACTAA AACTGCCAGT CCAGATCAAA CAGAAATGAC CATTGAAGGT
    TTGCAACCCA CTGTGGAGTA CGTGGTTAGT GTTTATGCTC AGAACCGGAA CGGAGAAAGC
    CAGCCCCTGG TTCAAACTGC AGTGACCACC ATTCCTGCGC CCACCAATCT GAAGTTCAGT
    CAGGTGACAC CCACCAGCTT TACTGCCCAG TGGATAGCAC CCAGTGTTCA GCTCACTGGC
    TACCGGGTGC GGGTGAACCC GAAAGAGAAG ACAGGACCAA TGAAAGAAAT CAACCTTTCT
    CCAGACAGCT CATCGGTGAT TGTGTCAGGA CTCATGGTGG CCACTAAATA CGAAGTCAGT
    GTCTATGCTC TCAAGGACAC ACTGACAAGC AGACCAGCCC AGGGAGTCAT CACTACTCTG
    GAGAATGTTA GCCCTCCAAG AAGGGCCCGT GTGACGGACG CTACAGAGAC CACCATCACT
    ATTAGCTGGA GAACAAAGAC AGAGACAATC ACTGGCTTCC AAGTCGATGC CATCCCAGCC
    AATGGCCAGA CCCCAGTTCA GAGGAGCATC AGCCCGGATG TTAGAAGCTA CACCATTACA
    GGTTTACAGC CAGGCACTGA CTACAAGATC CACCTGTACA CTCTGAACGA CAATGCCCGG
    AGCTCGCCCG TGATCATCGA TGCCTCCACC GCCATCGACG CACCATCCAA CCTGCGGTTC
    CTGACCACCA CACCCAACTC CTTGCTGGTG TCATGGCAGG CGCCCCGTGC CAGGATTACT
    GGCTACATTA TCAAGTATGA GAAGCCTGGA TCCCCTCCCA GAGAAGTGGT CCCTCGGCCC
    CGCCCTGGTG TCACGGAGGC CACCATTACT GGTCTGGAGC CAGGAACCGA GTACACCATC
    TACGTCATTG CCCTGAAGAA CAATCAGAAG AGTGAGCCCC TGATTGGGAG GAAGAAGACA
    GATGAGCTTC CCCAACTGGT TACCCTTCCA CACCCCAATC TTCATGGACC AGAGATCTTG
    GATGTTCCCT CCACAGTTCA AAAGACCCCC TTCATCACCA ACCCTGGGTA TGACACCGAA
    AATGGTATTC AGCTTCCTGG CACAACCCAC CAGCAACCCA GTGTTGGGCA ACAAATGATC
    TTTGAGGAAC ATGGCTTTAG GCGGACAACG CCACCCACTG CGGCCACCCC CGTCAGGCTT
    AGGCCAAGAC CATACCTGCC GAATGTAGAT GAGGAGGTCC AAATCGGTCA TGTTCCCAGG
    GGAGACGTAG ACTACCACCT CTATCCTCAT GTTCCGGGCC TCAATCCAAA TGCCTCTACA
    GGACAGGAAG CTCTCTCTCA GACAACCATC TCTTGGACGC CGTTCCAGGA GAGTTCTGAG
    TACATCATTT CATGCCAACC AGTTGGCACC GACGAAGAGC CCTTACAGTT CCAAGTTCCT
    GGAACTTCTA CCAGTGCGAC TCTGACTGGC CTTACCAGAG GGGTCACCTA CAACATCATA
    GTGGAGGCAC TGCAGAACCA GAGGAGGCAC AAGGTTCGGG AAGAGGTTGT GACTGTGGGC
    AACGCTGAAT GGTGCCATGA CAACGGTGTC AACTACAAGA TCGGAGAGAA GTGGGATCGG
    CAGGGAGAAA ATGGCCAGCG GATGAGCTGC ACATGCCTCG GGAATGGAAA GGGAGAATTC
    AAGTGTGATC CCCATGAAGC AACGTGCTAT GACGATGGGA AGACCTACCA TGTAGGAGAA
    CAGTGGCAGA AAGAATATCT CGGAGCCATT TGCTCCTGCA CGTGTTTCGG AGGCCAGCGG
    GGCTGGCGCT GTGACAACTG CCGTAGACCT GGGGCTGCTG AACCCAGTCC CGATGGCACC
    ACCGGCCACA CCTACAACCA GTATACACAG AGATACAATC AGAGAACAAA CACTAACGTA
    AATTGCCCCA TTGAGTGCTT CATGCCGCTA GATGTGCAAG CTGACAGAGA CGATTCTCGA
    GAGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017171337.1
    CDS299..7024
    Translation

    Target ORF information:

    RefSeq Version XM_017315848.2
    Organism Mus musculus(house mouse)
    Definition Mus musculus fibronectin 1 (Fn1), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017315848.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    ATGCTCAGGG GTCCGGGACC CGGGCGGCTG CTGCTGCTGG CAGTCCTGTG CCTGGGGACC 
    TCGGTGCGCT GCACCGAAGC CGGGAAGAGC AAGAGGCAGG CTCAGCAAAT CGTGCAGCCT
    CAATCCCCGG TGGCTGTCAG TCAGAGCAAG CCTGGCTGTT TTGACAATGG GAAGCACTAT
    CAGATAAATC AGCAGTGGGA ACGGACCTAC CTAGGCAACG CCCTGGTTTG TACCTGCTAT
    GGAGGAAGCC GGGGTTTTAA CTGCGAGAGC AAGCCTGAGC CTGAAGAGAC TTGCTTTGAC
    AAATACACTG GGAACACTTA CAAAGTGGGT GACACTTATG AGCGCCCTAA AGATTCCATG
    ATCTGGGACT GTACCTGCAT CGGGGCTGGG AGAGGCAGGA TCAGCTGTAC CATTGCAAAT
    CGCTGCCATG AAGGGGGTCA GTCCTACAAG ATTGGCGACA AGTGGAGGAG GCCACATGAG
    ACTGGTGGCT ACATGTTAGA GTGTCTGTGT CTGGGAAATG GAAAAGGGGA ATGGACCTGC
    AAACCTATAG CTGAGAAGTG TTTTGATCAT GCTGCTGGGA CGTCCTACGT CGTGGGGGAG
    ACCTGGGAAA AGCCCTACCA AGGCTGGATG ATGGTGGACT GTACTTGTCT AGGCGAAGGC
    AATGGACGCA TCACCTGTAC CTCCAGAAAC AGATGCAACG ATCAGGACAC CCGGACATCC
    TATAGGATTG GAGACACGTG GAGCAAGAAG GACAACCGAG GAAACCTGCT TCAGTGTGTC
    TGCACAGGCA ATGGCAGAGG GGAGTGGAAG TGTGAGCGAC ATGCTCTACA AAGTGCTTCA
    GCCGGATCTG GCTCCTTCAC TGATGTCCGA ACAGCTATTT ACCAACCGCA GACTCACCCC
    CAGCCCGCTC CCTACGGCCA CTGTGTCACC GACAGTGGTG TGGTCTACTC TGTGGGAATG
    CAGTGGCTGA AGTCGCAAGG AAACAAGCAA ATGCTGTGCA CGTGCCTGGG CAATGGCGTC
    AGCTGCCAGG AGACAGCCGT GACCCAGACT TATGGTGGCA ATTCAAACGG GGAGCCCTGT
    GTCCTCCCGT TCACCTACAA CGGTAGGACC TTCTATTCCT GCACCACCGA AGGGCGGCAA
    GACGGACATC TGTGGTGTAG CACAACTTCC AATTACGAAC AAGACCAGAA GTATTCCTTC
    TGCACAGACC ATGCGGTTTT GGTTCAGACT CGAGGCGGAA ATTCCAATGG TGCTCTGTGC
    CACTTCCCCT TCCTGTACAA CAACCGGAAT TACACCGACT GTACTTCTGA GGGTCGCAGG
    GACAACATGA AATGGTGCGG CACCACCCAG AACTACGATG CCGATCAGAA GTTTGGATTC
    TGCCCAATGG CTGCCCACGA GGAGATCTGC ACAACCAATG AAGGGGTCAT GTATCGCATT
    GGGGATCAGT GGGATAAGCA GCATGACCTG GGCCACATGA TGAGGTGCAC GTGTGTGGGG
    AACGGTCGTG GAGAATGGGC CTGCATCCCC TACTCCCAGC TCCGAGACCA GTGCATCGTT
    GATGACATTA CTTACAATGT GAACGACACG TTCCACAAGC GTCACGAGGA GGGACATATG
    CTGAACTGTA CCTGCTTTGG TCAGGGCCGG GGCAGATGGA AGTGTGACCC CATTGACCAG
    TGCCAAGATT CAGAGACCCG GACATTTTAC CAGATTGGTG ACTCCTGGGA GAAGTTTGTG
    CATGGTGTCC GATACCAGTG TTACTGCTAC GGCCGTGGCA TCGGGGAGTG GCACTGTCAA
    CCTCTGCAGA CCTACCCAGG CACAACTGGA CCTGTCCAAG TAATTATCAC GGAGACCCCC
    AGCCAGCCCA ATTCCCACCC CATCCAGTGG AATGCCCCGG AGCCTTCACA CATCACCAAG
    TACATTCTCA GATGGAGACC TAAAACCTCT ACGGGTCGCT GGAAGGAAGC TACCATTCCT
    GGCCACCTTA ACTCCTATAC CATCAAAGGC CTGACCCCAG GTGTGATCTA TGAGGGACAG
    CTCATCAGCA TCCAGCAGTA TGGCCACAGA GAAGTGACAC GCTTCGACTT CACCACCAGC
    GCCAGCACCC CTGTGACCAG CAACACGGTG ACCGGAGAGA CTGCGCCCTA CTCTCCTGTT
    GTGGCCACTT CTGAATCTGT AACTGAAATC ACAGCCAGCA GCTTTGTGGT CTCATGGGTC
    TCAGCCTCCG ACACCGTGTC AGGCTTCCGG GTGGAGTATG AGCTGAGCGA GGAGGGAGAT
    GAACCACAGT ACCTTGATCT CCCAAGCACG GCCACTTCCG TGAACATTCC TGACCTGCTC
    CCGGGCAGAA AGTACATTGT CAATGTCTAT CAGATATCTG AGGAGGGAAA ACAGAGCTTG
    ATCCTGTCTA CCTCACAGAC TACAGCACCT GACGCTCCTC CAGACCCTAC CGTGGACCAG
    GTTGATGATA CTTCCATTGT TGTTCGGTGG AGTAGACCCC AGGCACCTAT CACAGGGTAT
    AGAATTGTCT ATTCACCTTC AGTAGAAGGC AGTAGCACAG AGCTCAACCT CCCTGAAACG
    GCCAACTCCG TCACCCTCAG CGACCTGCAG CCCGGTGTTC AGTACAACAT CACTATCTAT
    GCTGTGGAGG AGAACCAGGA GAGCACACCC GTTTTCATCC AACAAGAGAC CACTGGCACC
    CCAAGATCTG ATAACGTCCC CCCTCCGACG GACCTACAGT TTGTGGAACT GACTGATGTG
    AAAGTCACCA TCATGTGGAC CCCTCCTGAT AGTGTGGTGT CTGGATACCG TGTGGAGGTC
    CTGCCTGTCA GCCTGCCCGG GGAACATGGG CAGAGGCTGC CTGTCAACAG AAATACCTTT
    GCTGAAATCA CTGGGCTGTC CCCTGGGGTC ACGTACCTCT TCAAAGTCTT TGCTGTGCAC
    CAGGGCAGGG AAAGCAATCC TCTGACGGCA CAACAGACCA CCAAACTCGA CGCTCCCACT
    AACCTCCAGT TTGTCAATGA AACTGACAGA ACAGTTCTGG TAACGTGGAC TCCACCTCGA
    GCCCGTATAG CAGGCTACCG ACTGACCGCG GGCCTGACCC GAGGAGGCCA GCCCAAGCAG
    TACAATGTGG GACCCTTGGC CTCCAAGTAT CCCCTGAGAA ATCTGCAGCC TGGGTCTGAG
    TACACCGTGA CCTTGGTGGC TGTGAAAGGG AACCAGCAGA GTCCCAAAGC CACCGGAGTC
    TTTACTACCC TGCAGCCTCT GCGCTCCATT CCACCTTACA ACACCGAGGT GACAGAGACC
    ACAATTGTGA TCACCTGGAC CCCCGCTCCA AGGATTGGCT TCAAGCTGGG TGTACGACCG
    AGCCAGGGAG GTGAGGCACC CCGAGAAGTG ACTTCAGACT CTGGGAGCAT TGTTGTGTCT
    GGCTTGACTC CAGGCGTGGA ATACACTTAC ACCATCCAAG TCCTGCGAGA TGGCCAGGAG
    AGAGATGCAC CGATTGTCAA CAGAGTAGTG ACACCGCTGT CTCCACCGAC CAACTTGCAT
    CTGGAGGCAA ACCCTGACAC TGGAGTGCTT ACTGTCTCCT GGGAGAGGAG CACTACCCCA
    GATATCACTG GCTACAGAAT AACTACTACC CCCACGAACG GGCAGCAGGG GACCTCTCTG
    GAAGAAGTGG TCCATGCTGA TCAGAGTTCC TGCACTTTTG AGAACCTGAA TCCTGGCCTG
    GAGTACAACG TCAGTGTTTA CACTGTCAAA GATGACAAGG AAAGTGCCCC TATCTCTGAT
    ACCGTTGTCC CAGCTGTCCC TCCTCCCACG GATCTGCGAT TCACCAATAT CGGTCCAGAC
    ACGATGCGGG TCACTTGGGC CCCGCCTCCG TCCATCGAGC TGACCAACCT CTTGGTGCGC
    TACTCACCCG TGAAGAATGA AGAGGACGTT GCAGAGCTAT CCATTTCACC TTCAGACAAT
    GCCGTGGTCC TAACAAATCT CCTGCCTGGG ACAGAATACT TAGTCAGTGT CTCCAGTGTC
    TACGAACAAC ATGAGAGCAT CCCTCTCCGG GGAAGGCAGA AAACAGGTCT CGATTCCCCA
    ACTGGTTTTG ATTCTTCTGA TATCACCGCC AACTCATTCA CTGTCCACTG GGTGGCTCCT
    CGGGCCCCCA TCACCGGCTA CATCATCCGC CATCACGCCG AGCATTCTGT CGGAAGACCC
    AGGCAGGATC GAGTGCCGCC CTCGCGGAAT TCCATCACCC TCACCAACCT TAATCCGGGC
    ACCGAGTACG TTGTCAGCAT CATTGCTGTT AATGGCAGAG AGGAGAGCCC GCCACTGATT
    GGCCAGCAAG CCACAGTTTC TGATATTCCG AGAGATCTGG AGGTCATTGC CTCCACCCCC
    ACCAGCCTGC TCATCAGTTG GGAACCCCCT GCCGTCTCTG TGCGCTATTA CAGAATCACC
    TACGGAGAGA CAGGAGGAAA TAGCCCTGTC CAGGAGTTCA CTGTGCCCGG AAGCAAGTCC
    ACAGCCACCA TCAACAACAT TAAACCAGGA GCAGACTACA CCATCACCCT GTATGCTGTC
    ACTGGCCGTG GGGACAGTCC AGCAAGCAGC AAGCCAGTTT CCATCAATTA TAAAACAGAA
    ATTGACAAGC CGTCCCAGAT GCAGGTGACA GATGTCCAGG ACAACAGCAT CAGTGTCAGG
    TGGCTGCCTT CAACTTCTCC TGTGACAGGC TACAGAGTGA CCACCACTCC CAAAAATGGC
    CTAGGACCAT CAAAAACTAA AACTGCCAGT CCAGATCAAA CAGAAATGAC CATTGAAGGT
    TTGCAACCCA CTGTGGAGTA CGTGGTTAGT GTTTATGCTC AGAACCGGAA CGGAGAAAGC
    CAGCCCCTGG TTCAAACTGC AGTGACCACC ATTCCTGCGC CCACCAATCT GAAGTTCAGT
    CAGGTGACAC CCACCAGCTT TACTGCCCAG TGGATAGCAC CCAGTGTTCA GCTCACTGGC
    TACCGGGTGC GGGTGAACCC GAAAGAGAAG ACAGGACCAA TGAAAGAAAT CAACCTTTCT
    CCAGACAGCT CATCGGTGAT TGTGTCAGGA CTCATGGTGG CCACTAAATA CGAAGTCAGT
    GTCTATGCTC TCAAGGACAC ACTGACAAGC AGACCAGCCC AGGGAGTCAT CACTACTCTG
    GAGAATGTTA GCCCTCCAAG AAGGGCCCGT GTGACGGACG CTACAGAGAC CACCATCACT
    ATTAGCTGGA GAACAAAGAC AGAGACAATC ACTGGCTTCC AAGTCGATGC CATCCCAGCC
    AATGGCCAGA CCCCAGTTCA GAGGAGCATC AGCCCGGATG TTAGAAGCTA CACCATTACA
    GGTTTACAGC CAGGCACTGA CTACAAGATC CACCTGTACA CTCTGAACGA CAATGCCCGG
    AGCTCGCCCG TGATCATCGA TGCCTCCACC GCCATCGACG CACCATCCAA CCTGCGGTTC
    CTGACCACCA CACCCAACTC CTTGCTGGTG TCATGGCAGG CGCCCCGTGC CAGGATTACT
    GGCTACATTA TCAAGTATGA GAAGCCTGGA TCCCCTCCCA GAGAAGTGGT CCCTCGGCCC
    CGCCCTGGTG TCACGGAGGC CACCATTACT GGTCTGGAGC CAGGAACCGA GTACACCATC
    TACGTCATTG CCCTGAAGAA CAATCAGAAG AGTGAGCCCC TGATTGGGAG GAAGAAGACA
    GATGAGCTTC CCCAACTGGT TACCCTTCCA CACCCCAATC TTCATGGACC AGAGATCTTG
    GATGTTCCCT CCACAGTTCA AAAGACCCCC TTCATCACCA ACCCTGGGTA TGACACCGAA
    AATGGTATTC AGCTTCCTGG CACAACCCAC CAGCAACCCA GTGTTGGGCA ACAAATGATC
    TTTGAGGAAC ATGGCTTTAG GCGGACAACG CCACCCACTG CGGCCACCCC CGTCAGGCTT
    AGGCCAAGAC CATACCTGCC GAATGTAGAT GAGGAGGTCC AAATCGGTCA TGTTCCCAGG
    GGAGACGTAG ACTACCACCT CTATCCTCAT GTTCCGGGCC TCAATCCAAA TGCCTCTACA
    GGACAGGAAG CTCTCTCTCA GACAACCATC TCTTGGACGC CGTTCCAGGA GAGTTCTGAG
    TACATCATTT CATGCCAACC AGTTGGCACC GACGAAGAGC CCTTACAGTT CCAAGTTCCT
    GGAACTTCTA CCAGTGCGAC TCTGACTGGC CTTACCAGAG GGGTCACCTA CAACATCATA
    GTGGAGGCAC TGCAGAACCA GAGGAGGCAC AAGGTTCGGG AAGAGGTTGT GACTGTGGGC
    AACGCTGAAT GGTGCCATGA CAACGGTGTC AACTACAAGA TCGGAGAGAA GTGGGATCGG
    CAGGGAGAAA ATGGCCAGCG GATGAGCTGC ACATGCCTCG GGAATGGAAA GGGAGAATTC
    AAGTGTGATC CCCATGAAGC AACGTGCTAT GACGATGGGA AGACCTACCA TGTAGGAGAA
    CAGTGGCAGA AAGAATATCT CGGAGCCATT TGCTCCTGCA CGTGTTTCGG AGGCCAGCGG
    GGCTGGCGCT GTGACAACTG CCGTAGACCT GGGGCTGCTG AACCCAGTCC CGATGGCACC
    ACCGGCCACA CCTACAACCA GTATACACAG AGATACAATC AGAGAACAAA CACTAACGTA
    AATTGCCCCA TTGAGTGCTT CATGCCGCTA GATGTGCAAG CTGACAGAGA CGATTCTCGA
    GAGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu76978
    Clone ID Related Accession (Same CDS sequence) XM_017315848.2 , XM_017315848.1
    Accession Version XM_017315848.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6726bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-21
    Organism Mus musculus(house mouse)
    Product fibronectin isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    ATGCTCAGGG GTCCGGGACC CGGGCGGCTG CTGCTGCTGG CAGTCCTGTG CCTGGGGACC 
    TCGGTGCGCT GCACCGAAGC CGGGAAGAGC AAGAGGCAGG CTCAGCAAAT CGTGCAGCCT
    CAATCCCCGG TGGCTGTCAG TCAGAGCAAG CCTGGCTGTT TTGACAATGG GAAGCACTAT
    CAGATAAATC AGCAGTGGGA ACGGACCTAC CTAGGCAACG CCCTGGTTTG TACCTGCTAT
    GGAGGAAGCC GGGGTTTTAA CTGCGAGAGC AAGCCTGAGC CTGAAGAGAC TTGCTTTGAC
    AAATACACTG GGAACACTTA CAAAGTGGGT GACACTTATG AGCGCCCTAA AGATTCCATG
    ATCTGGGACT GTACCTGCAT CGGGGCTGGG AGAGGCAGGA TCAGCTGTAC CATTGCAAAT
    CGCTGCCATG AAGGGGGTCA GTCCTACAAG ATTGGCGACA AGTGGAGGAG GCCACATGAG
    ACTGGTGGCT ACATGTTAGA GTGTCTGTGT CTGGGAAATG GAAAAGGGGA ATGGACCTGC
    AAACCTATAG CTGAGAAGTG TTTTGATCAT GCTGCTGGGA CGTCCTACGT CGTGGGGGAG
    ACCTGGGAAA AGCCCTACCA AGGCTGGATG ATGGTGGACT GTACTTGTCT AGGCGAAGGC
    AATGGACGCA TCACCTGTAC CTCCAGAAAC AGATGCAACG ATCAGGACAC CCGGACATCC
    TATAGGATTG GAGACACGTG GAGCAAGAAG GACAACCGAG GAAACCTGCT TCAGTGTGTC
    TGCACAGGCA ATGGCAGAGG GGAGTGGAAG TGTGAGCGAC ATGCTCTACA AAGTGCTTCA
    GCCGGATCTG GCTCCTTCAC TGATGTCCGA ACAGCTATTT ACCAACCGCA GACTCACCCC
    CAGCCCGCTC CCTACGGCCA CTGTGTCACC GACAGTGGTG TGGTCTACTC TGTGGGAATG
    CAGTGGCTGA AGTCGCAAGG AAACAAGCAA ATGCTGTGCA CGTGCCTGGG CAATGGCGTC
    AGCTGCCAGG AGACAGCCGT GACCCAGACT TATGGTGGCA ATTCAAACGG GGAGCCCTGT
    GTCCTCCCGT TCACCTACAA CGGTAGGACC TTCTATTCCT GCACCACCGA AGGGCGGCAA
    GACGGACATC TGTGGTGTAG CACAACTTCC AATTACGAAC AAGACCAGAA GTATTCCTTC
    TGCACAGACC ATGCGGTTTT GGTTCAGACT CGAGGCGGAA ATTCCAATGG TGCTCTGTGC
    CACTTCCCCT TCCTGTACAA CAACCGGAAT TACACCGACT GTACTTCTGA GGGTCGCAGG
    GACAACATGA AATGGTGCGG CACCACCCAG AACTACGATG CCGATCAGAA GTTTGGATTC
    TGCCCAATGG CTGCCCACGA GGAGATCTGC ACAACCAATG AAGGGGTCAT GTATCGCATT
    GGGGATCAGT GGGATAAGCA GCATGACCTG GGCCACATGA TGAGGTGCAC GTGTGTGGGG
    AACGGTCGTG GAGAATGGGC CTGCATCCCC TACTCCCAGC TCCGAGACCA GTGCATCGTT
    GATGACATTA CTTACAATGT GAACGACACG TTCCACAAGC GTCACGAGGA GGGACATATG
    CTGAACTGTA CCTGCTTTGG TCAGGGCCGG GGCAGATGGA AGTGTGACCC CATTGACCAG
    TGCCAAGATT CAGAGACCCG GACATTTTAC CAGATTGGTG ACTCCTGGGA GAAGTTTGTG
    CATGGTGTCC GATACCAGTG TTACTGCTAC GGCCGTGGCA TCGGGGAGTG GCACTGTCAA
    CCTCTGCAGA CCTACCCAGG CACAACTGGA CCTGTCCAAG TAATTATCAC GGAGACCCCC
    AGCCAGCCCA ATTCCCACCC CATCCAGTGG AATGCCCCGG AGCCTTCACA CATCACCAAG
    TACATTCTCA GATGGAGACC TAAAACCTCT ACGGGTCGCT GGAAGGAAGC TACCATTCCT
    GGCCACCTTA ACTCCTATAC CATCAAAGGC CTGACCCCAG GTGTGATCTA TGAGGGACAG
    CTCATCAGCA TCCAGCAGTA TGGCCACAGA GAAGTGACAC GCTTCGACTT CACCACCAGC
    GCCAGCACCC CTGTGACCAG CAACACGGTG ACCGGAGAGA CTGCGCCCTA CTCTCCTGTT
    GTGGCCACTT CTGAATCTGT AACTGAAATC ACAGCCAGCA GCTTTGTGGT CTCATGGGTC
    TCAGCCTCCG ACACCGTGTC AGGCTTCCGG GTGGAGTATG AGCTGAGCGA GGAGGGAGAT
    GAACCACAGT ACCTTGATCT CCCAAGCACG GCCACTTCCG TGAACATTCC TGACCTGCTC
    CCGGGCAGAA AGTACATTGT CAATGTCTAT CAGATATCTG AGGAGGGAAA ACAGAGCTTG
    ATCCTGTCTA CCTCACAGAC TACAGCACCT GACGCTCCTC CAGACCCTAC CGTGGACCAG
    GTTGATGATA CTTCCATTGT TGTTCGGTGG AGTAGACCCC AGGCACCTAT CACAGGGTAT
    AGAATTGTCT ATTCACCTTC AGTAGAAGGC AGTAGCACAG AGCTCAACCT CCCTGAAACG
    GCCAACTCCG TCACCCTCAG CGACCTGCAG CCCGGTGTTC AGTACAACAT CACTATCTAT
    GCTGTGGAGG AGAACCAGGA GAGCACACCC GTTTTCATCC AACAAGAGAC CACTGGCACC
    CCAAGATCTG ATAACGTCCC CCCTCCGACG GACCTACAGT TTGTGGAACT GACTGATGTG
    AAAGTCACCA TCATGTGGAC CCCTCCTGAT AGTGTGGTGT CTGGATACCG TGTGGAGGTC
    CTGCCTGTCA GCCTGCCCGG GGAACATGGG CAGAGGCTGC CTGTCAACAG AAATACCTTT
    GCTGAAATCA CTGGGCTGTC CCCTGGGGTC ACGTACCTCT TCAAAGTCTT TGCTGTGCAC
    CAGGGCAGGG AAAGCAATCC TCTGACGGCA CAACAGACCA CCAAACTCGA CGCTCCCACT
    AACCTCCAGT TTGTCAATGA AACTGACAGA ACAGTTCTGG TAACGTGGAC TCCACCTCGA
    GCCCGTATAG CAGGCTACCG ACTGACCGCG GGCCTGACCC GAGGAGGCCA GCCCAAGCAG
    TACAATGTGG GACCCTTGGC CTCCAAGTAT CCCCTGAGAA ATCTGCAGCC TGGGTCTGAG
    TACACCGTGA CCTTGGTGGC TGTGAAAGGG AACCAGCAGA GTCCCAAAGC CACCGGAGTC
    TTTACTACCC TGCAGCCTCT GCGCTCCATT CCACCTTACA ACACCGAGGT GACAGAGACC
    ACAATTGTGA TCACCTGGAC CCCCGCTCCA AGGATTGGCT TCAAGCTGGG TGTACGACCG
    AGCCAGGGAG GTGAGGCACC CCGAGAAGTG ACTTCAGACT CTGGGAGCAT TGTTGTGTCT
    GGCTTGACTC CAGGCGTGGA ATACACTTAC ACCATCCAAG TCCTGCGAGA TGGCCAGGAG
    AGAGATGCAC CGATTGTCAA CAGAGTAGTG ACACCGCTGT CTCCACCGAC CAACTTGCAT
    CTGGAGGCAA ACCCTGACAC TGGAGTGCTT ACTGTCTCCT GGGAGAGGAG CACTACCCCA
    GATATCACTG GCTACAGAAT AACTACTACC CCCACGAACG GGCAGCAGGG GACCTCTCTG
    GAAGAAGTGG TCCATGCTGA TCAGAGTTCC TGCACTTTTG AGAACCTGAA TCCTGGCCTG
    GAGTACAACG TCAGTGTTTA CACTGTCAAA GATGACAAGG AAAGTGCCCC TATCTCTGAT
    ACCGTTGTCC CAGCTGTCCC TCCTCCCACG GATCTGCGAT TCACCAATAT CGGTCCAGAC
    ACGATGCGGG TCACTTGGGC CCCGCCTCCG TCCATCGAGC TGACCAACCT CTTGGTGCGC
    TACTCACCCG TGAAGAATGA AGAGGACGTT GCAGAGCTAT CCATTTCACC TTCAGACAAT
    GCCGTGGTCC TAACAAATCT CCTGCCTGGG ACAGAATACT TAGTCAGTGT CTCCAGTGTC
    TACGAACAAC ATGAGAGCAT CCCTCTCCGG GGAAGGCAGA AAACAGGTCT CGATTCCCCA
    ACTGGTTTTG ATTCTTCTGA TATCACCGCC AACTCATTCA CTGTCCACTG GGTGGCTCCT
    CGGGCCCCCA TCACCGGCTA CATCATCCGC CATCACGCCG AGCATTCTGT CGGAAGACCC
    AGGCAGGATC GAGTGCCGCC CTCGCGGAAT TCCATCACCC TCACCAACCT TAATCCGGGC
    ACCGAGTACG TTGTCAGCAT CATTGCTGTT AATGGCAGAG AGGAGAGCCC GCCACTGATT
    GGCCAGCAAG CCACAGTTTC TGATATTCCG AGAGATCTGG AGGTCATTGC CTCCACCCCC
    ACCAGCCTGC TCATCAGTTG GGAACCCCCT GCCGTCTCTG TGCGCTATTA CAGAATCACC
    TACGGAGAGA CAGGAGGAAA TAGCCCTGTC CAGGAGTTCA CTGTGCCCGG AAGCAAGTCC
    ACAGCCACCA TCAACAACAT TAAACCAGGA GCAGACTACA CCATCACCCT GTATGCTGTC
    ACTGGCCGTG GGGACAGTCC AGCAAGCAGC AAGCCAGTTT CCATCAATTA TAAAACAGAA
    ATTGACAAGC CGTCCCAGAT GCAGGTGACA GATGTCCAGG ACAACAGCAT CAGTGTCAGG
    TGGCTGCCTT CAACTTCTCC TGTGACAGGC TACAGAGTGA CCACCACTCC CAAAAATGGC
    CTAGGACCAT CAAAAACTAA AACTGCCAGT CCAGATCAAA CAGAAATGAC CATTGAAGGT
    TTGCAACCCA CTGTGGAGTA CGTGGTTAGT GTTTATGCTC AGAACCGGAA CGGAGAAAGC
    CAGCCCCTGG TTCAAACTGC AGTGACCACC ATTCCTGCGC CCACCAATCT GAAGTTCAGT
    CAGGTGACAC CCACCAGCTT TACTGCCCAG TGGATAGCAC CCAGTGTTCA GCTCACTGGC
    TACCGGGTGC GGGTGAACCC GAAAGAGAAG ACAGGACCAA TGAAAGAAAT CAACCTTTCT
    CCAGACAGCT CATCGGTGAT TGTGTCAGGA CTCATGGTGG CCACTAAATA CGAAGTCAGT
    GTCTATGCTC TCAAGGACAC ACTGACAAGC AGACCAGCCC AGGGAGTCAT CACTACTCTG
    GAGAATGTTA GCCCTCCAAG AAGGGCCCGT GTGACGGACG CTACAGAGAC CACCATCACT
    ATTAGCTGGA GAACAAAGAC AGAGACAATC ACTGGCTTCC AAGTCGATGC CATCCCAGCC
    AATGGCCAGA CCCCAGTTCA GAGGAGCATC AGCCCGGATG TTAGAAGCTA CACCATTACA
    GGTTTACAGC CAGGCACTGA CTACAAGATC CACCTGTACA CTCTGAACGA CAATGCCCGG
    AGCTCGCCCG TGATCATCGA TGCCTCCACC GCCATCGACG CACCATCCAA CCTGCGGTTC
    CTGACCACCA CACCCAACTC CTTGCTGGTG TCATGGCAGG CGCCCCGTGC CAGGATTACT
    GGCTACATTA TCAAGTATGA GAAGCCTGGA TCCCCTCCCA GAGAAGTGGT CCCTCGGCCC
    CGCCCTGGTG TCACGGAGGC CACCATTACT GGTCTGGAGC CAGGAACCGA GTACACCATC
    TACGTCATTG CCCTGAAGAA CAATCAGAAG AGTGAGCCCC TGATTGGGAG GAAGAAGACA
    GATGAGCTTC CCCAACTGGT TACCCTTCCA CACCCCAATC TTCATGGACC AGAGATCTTG
    GATGTTCCCT CCACAGTTCA AAAGACCCCC TTCATCACCA ACCCTGGGTA TGACACCGAA
    AATGGTATTC AGCTTCCTGG CACAACCCAC CAGCAACCCA GTGTTGGGCA ACAAATGATC
    TTTGAGGAAC ATGGCTTTAG GCGGACAACG CCACCCACTG CGGCCACCCC CGTCAGGCTT
    AGGCCAAGAC CATACCTGCC GAATGTAGAT GAGGAGGTCC AAATCGGTCA TGTTCCCAGG
    GGAGACGTAG ACTACCACCT CTATCCTCAT GTTCCGGGCC TCAATCCAAA TGCCTCTACA
    GGACAGGAAG CTCTCTCTCA GACAACCATC TCTTGGACGC CGTTCCAGGA GAGTTCTGAG
    TACATCATTT CATGCCAACC AGTTGGCACC GACGAAGAGC CCTTACAGTT CCAAGTTCCT
    GGAACTTCTA CCAGTGCGAC TCTGACTGGC CTTACCAGAG GGGTCACCTA CAACATCATA
    GTGGAGGCAC TGCAGAACCA GAGGAGGCAC AAGGTTCGGG AAGAGGTTGT GACTGTGGGC
    AACGCTGAAT GGTGCCATGA CAACGGTGTC AACTACAAGA TCGGAGAGAA GTGGGATCGG
    CAGGGAGAAA ATGGCCAGCG GATGAGCTGC ACATGCCTCG GGAATGGAAA GGGAGAATTC
    AAGTGTGATC CCCATGAAGC AACGTGCTAT GACGATGGGA AGACCTACCA TGTAGGAGAA
    CAGTGGCAGA AAGAATATCT CGGAGCCATT TGCTCCTGCA CGTGTTTCGG AGGCCAGCGG
    GGCTGGCGCT GTGACAACTG CCGTAGACCT GGGGCTGCTG AACCCAGTCC CGATGGCACC
    ACCGGCCACA CCTACAACCA GTATACACAG AGATACAATC AGAGAACAAA CACTAACGTA
    AATTGCCCCA TTGAGTGCTT CATGCCGCTA GATGTGCAAG CTGACAGAGA CGATTCTCGA
    GAGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017171337.1
    CDS248..6973
    Translation

    Target ORF information:

    RefSeq Version XM_017315848.1
    Organism Mus musculus(house mouse)
    Definition Mus musculus fibronectin 1 (Fn1), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017315848.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    ATGCTCAGGG GTCCGGGACC CGGGCGGCTG CTGCTGCTGG CAGTCCTGTG CCTGGGGACC 
    TCGGTGCGCT GCACCGAAGC CGGGAAGAGC AAGAGGCAGG CTCAGCAAAT CGTGCAGCCT
    CAATCCCCGG TGGCTGTCAG TCAGAGCAAG CCTGGCTGTT TTGACAATGG GAAGCACTAT
    CAGATAAATC AGCAGTGGGA ACGGACCTAC CTAGGCAACG CCCTGGTTTG TACCTGCTAT
    GGAGGAAGCC GGGGTTTTAA CTGCGAGAGC AAGCCTGAGC CTGAAGAGAC TTGCTTTGAC
    AAATACACTG GGAACACTTA CAAAGTGGGT GACACTTATG AGCGCCCTAA AGATTCCATG
    ATCTGGGACT GTACCTGCAT CGGGGCTGGG AGAGGCAGGA TCAGCTGTAC CATTGCAAAT
    CGCTGCCATG AAGGGGGTCA GTCCTACAAG ATTGGCGACA AGTGGAGGAG GCCACATGAG
    ACTGGTGGCT ACATGTTAGA GTGTCTGTGT CTGGGAAATG GAAAAGGGGA ATGGACCTGC
    AAACCTATAG CTGAGAAGTG TTTTGATCAT GCTGCTGGGA CGTCCTACGT CGTGGGGGAG
    ACCTGGGAAA AGCCCTACCA AGGCTGGATG ATGGTGGACT GTACTTGTCT AGGCGAAGGC
    AATGGACGCA TCACCTGTAC CTCCAGAAAC AGATGCAACG ATCAGGACAC CCGGACATCC
    TATAGGATTG GAGACACGTG GAGCAAGAAG GACAACCGAG GAAACCTGCT TCAGTGTGTC
    TGCACAGGCA ATGGCAGAGG GGAGTGGAAG TGTGAGCGAC ATGCTCTACA AAGTGCTTCA
    GCCGGATCTG GCTCCTTCAC TGATGTCCGA ACAGCTATTT ACCAACCGCA GACTCACCCC
    CAGCCCGCTC CCTACGGCCA CTGTGTCACC GACAGTGGTG TGGTCTACTC TGTGGGAATG
    CAGTGGCTGA AGTCGCAAGG AAACAAGCAA ATGCTGTGCA CGTGCCTGGG CAATGGCGTC
    AGCTGCCAGG AGACAGCCGT GACCCAGACT TATGGTGGCA ATTCAAACGG GGAGCCCTGT
    GTCCTCCCGT TCACCTACAA CGGTAGGACC TTCTATTCCT GCACCACCGA AGGGCGGCAA
    GACGGACATC TGTGGTGTAG CACAACTTCC AATTACGAAC AAGACCAGAA GTATTCCTTC
    TGCACAGACC ATGCGGTTTT GGTTCAGACT CGAGGCGGAA ATTCCAATGG TGCTCTGTGC
    CACTTCCCCT TCCTGTACAA CAACCGGAAT TACACCGACT GTACTTCTGA GGGTCGCAGG
    GACAACATGA AATGGTGCGG CACCACCCAG AACTACGATG CCGATCAGAA GTTTGGATTC
    TGCCCAATGG CTGCCCACGA GGAGATCTGC ACAACCAATG AAGGGGTCAT GTATCGCATT
    GGGGATCAGT GGGATAAGCA GCATGACCTG GGCCACATGA TGAGGTGCAC GTGTGTGGGG
    AACGGTCGTG GAGAATGGGC CTGCATCCCC TACTCCCAGC TCCGAGACCA GTGCATCGTT
    GATGACATTA CTTACAATGT GAACGACACG TTCCACAAGC GTCACGAGGA GGGACATATG
    CTGAACTGTA CCTGCTTTGG TCAGGGCCGG GGCAGATGGA AGTGTGACCC CATTGACCAG
    TGCCAAGATT CAGAGACCCG GACATTTTAC CAGATTGGTG ACTCCTGGGA GAAGTTTGTG
    CATGGTGTCC GATACCAGTG TTACTGCTAC GGCCGTGGCA TCGGGGAGTG GCACTGTCAA
    CCTCTGCAGA CCTACCCAGG CACAACTGGA CCTGTCCAAG TAATTATCAC GGAGACCCCC
    AGCCAGCCCA ATTCCCACCC CATCCAGTGG AATGCCCCGG AGCCTTCACA CATCACCAAG
    TACATTCTCA GATGGAGACC TAAAACCTCT ACGGGTCGCT GGAAGGAAGC TACCATTCCT
    GGCCACCTTA ACTCCTATAC CATCAAAGGC CTGACCCCAG GTGTGATCTA TGAGGGACAG
    CTCATCAGCA TCCAGCAGTA TGGCCACAGA GAAGTGACAC GCTTCGACTT CACCACCAGC
    GCCAGCACCC CTGTGACCAG CAACACGGTG ACCGGAGAGA CTGCGCCCTA CTCTCCTGTT
    GTGGCCACTT CTGAATCTGT AACTGAAATC ACAGCCAGCA GCTTTGTGGT CTCATGGGTC
    TCAGCCTCCG ACACCGTGTC AGGCTTCCGG GTGGAGTATG AGCTGAGCGA GGAGGGAGAT
    GAACCACAGT ACCTTGATCT CCCAAGCACG GCCACTTCCG TGAACATTCC TGACCTGCTC
    CCGGGCAGAA AGTACATTGT CAATGTCTAT CAGATATCTG AGGAGGGAAA ACAGAGCTTG
    ATCCTGTCTA CCTCACAGAC TACAGCACCT GACGCTCCTC CAGACCCTAC CGTGGACCAG
    GTTGATGATA CTTCCATTGT TGTTCGGTGG AGTAGACCCC AGGCACCTAT CACAGGGTAT
    AGAATTGTCT ATTCACCTTC AGTAGAAGGC AGTAGCACAG AGCTCAACCT CCCTGAAACG
    GCCAACTCCG TCACCCTCAG CGACCTGCAG CCCGGTGTTC AGTACAACAT CACTATCTAT
    GCTGTGGAGG AGAACCAGGA GAGCACACCC GTTTTCATCC AACAAGAGAC CACTGGCACC
    CCAAGATCTG ATAACGTCCC CCCTCCGACG GACCTACAGT TTGTGGAACT GACTGATGTG
    AAAGTCACCA TCATGTGGAC CCCTCCTGAT AGTGTGGTGT CTGGATACCG TGTGGAGGTC
    CTGCCTGTCA GCCTGCCCGG GGAACATGGG CAGAGGCTGC CTGTCAACAG AAATACCTTT
    GCTGAAATCA CTGGGCTGTC CCCTGGGGTC ACGTACCTCT TCAAAGTCTT TGCTGTGCAC
    CAGGGCAGGG AAAGCAATCC TCTGACGGCA CAACAGACCA CCAAACTCGA CGCTCCCACT
    AACCTCCAGT TTGTCAATGA AACTGACAGA ACAGTTCTGG TAACGTGGAC TCCACCTCGA
    GCCCGTATAG CAGGCTACCG ACTGACCGCG GGCCTGACCC GAGGAGGCCA GCCCAAGCAG
    TACAATGTGG GACCCTTGGC CTCCAAGTAT CCCCTGAGAA ATCTGCAGCC TGGGTCTGAG
    TACACCGTGA CCTTGGTGGC TGTGAAAGGG AACCAGCAGA GTCCCAAAGC CACCGGAGTC
    TTTACTACCC TGCAGCCTCT GCGCTCCATT CCACCTTACA ACACCGAGGT GACAGAGACC
    ACAATTGTGA TCACCTGGAC CCCCGCTCCA AGGATTGGCT TCAAGCTGGG TGTACGACCG
    AGCCAGGGAG GTGAGGCACC CCGAGAAGTG ACTTCAGACT CTGGGAGCAT TGTTGTGTCT
    GGCTTGACTC CAGGCGTGGA ATACACTTAC ACCATCCAAG TCCTGCGAGA TGGCCAGGAG
    AGAGATGCAC CGATTGTCAA CAGAGTAGTG ACACCGCTGT CTCCACCGAC CAACTTGCAT
    CTGGAGGCAA ACCCTGACAC TGGAGTGCTT ACTGTCTCCT GGGAGAGGAG CACTACCCCA
    GATATCACTG GCTACAGAAT AACTACTACC CCCACGAACG GGCAGCAGGG GACCTCTCTG
    GAAGAAGTGG TCCATGCTGA TCAGAGTTCC TGCACTTTTG AGAACCTGAA TCCTGGCCTG
    GAGTACAACG TCAGTGTTTA CACTGTCAAA GATGACAAGG AAAGTGCCCC TATCTCTGAT
    ACCGTTGTCC CAGCTGTCCC TCCTCCCACG GATCTGCGAT TCACCAATAT CGGTCCAGAC
    ACGATGCGGG TCACTTGGGC CCCGCCTCCG TCCATCGAGC TGACCAACCT CTTGGTGCGC
    TACTCACCCG TGAAGAATGA AGAGGACGTT GCAGAGCTAT CCATTTCACC TTCAGACAAT
    GCCGTGGTCC TAACAAATCT CCTGCCTGGG ACAGAATACT TAGTCAGTGT CTCCAGTGTC
    TACGAACAAC ATGAGAGCAT CCCTCTCCGG GGAAGGCAGA AAACAGGTCT CGATTCCCCA
    ACTGGTTTTG ATTCTTCTGA TATCACCGCC AACTCATTCA CTGTCCACTG GGTGGCTCCT
    CGGGCCCCCA TCACCGGCTA CATCATCCGC CATCACGCCG AGCATTCTGT CGGAAGACCC
    AGGCAGGATC GAGTGCCGCC CTCGCGGAAT TCCATCACCC TCACCAACCT TAATCCGGGC
    ACCGAGTACG TTGTCAGCAT CATTGCTGTT AATGGCAGAG AGGAGAGCCC GCCACTGATT
    GGCCAGCAAG CCACAGTTTC TGATATTCCG AGAGATCTGG AGGTCATTGC CTCCACCCCC
    ACCAGCCTGC TCATCAGTTG GGAACCCCCT GCCGTCTCTG TGCGCTATTA CAGAATCACC
    TACGGAGAGA CAGGAGGAAA TAGCCCTGTC CAGGAGTTCA CTGTGCCCGG AAGCAAGTCC
    ACAGCCACCA TCAACAACAT TAAACCAGGA GCAGACTACA CCATCACCCT GTATGCTGTC
    ACTGGCCGTG GGGACAGTCC AGCAAGCAGC AAGCCAGTTT CCATCAATTA TAAAACAGAA
    ATTGACAAGC CGTCCCAGAT GCAGGTGACA GATGTCCAGG ACAACAGCAT CAGTGTCAGG
    TGGCTGCCTT CAACTTCTCC TGTGACAGGC TACAGAGTGA CCACCACTCC CAAAAATGGC
    CTAGGACCAT CAAAAACTAA AACTGCCAGT CCAGATCAAA CAGAAATGAC CATTGAAGGT
    TTGCAACCCA CTGTGGAGTA CGTGGTTAGT GTTTATGCTC AGAACCGGAA CGGAGAAAGC
    CAGCCCCTGG TTCAAACTGC AGTGACCACC ATTCCTGCGC CCACCAATCT GAAGTTCAGT
    CAGGTGACAC CCACCAGCTT TACTGCCCAG TGGATAGCAC CCAGTGTTCA GCTCACTGGC
    TACCGGGTGC GGGTGAACCC GAAAGAGAAG ACAGGACCAA TGAAAGAAAT CAACCTTTCT
    CCAGACAGCT CATCGGTGAT TGTGTCAGGA CTCATGGTGG CCACTAAATA CGAAGTCAGT
    GTCTATGCTC TCAAGGACAC ACTGACAAGC AGACCAGCCC AGGGAGTCAT CACTACTCTG
    GAGAATGTTA GCCCTCCAAG AAGGGCCCGT GTGACGGACG CTACAGAGAC CACCATCACT
    ATTAGCTGGA GAACAAAGAC AGAGACAATC ACTGGCTTCC AAGTCGATGC CATCCCAGCC
    AATGGCCAGA CCCCAGTTCA GAGGAGCATC AGCCCGGATG TTAGAAGCTA CACCATTACA
    GGTTTACAGC CAGGCACTGA CTACAAGATC CACCTGTACA CTCTGAACGA CAATGCCCGG
    AGCTCGCCCG TGATCATCGA TGCCTCCACC GCCATCGACG CACCATCCAA CCTGCGGTTC
    CTGACCACCA CACCCAACTC CTTGCTGGTG TCATGGCAGG CGCCCCGTGC CAGGATTACT
    GGCTACATTA TCAAGTATGA GAAGCCTGGA TCCCCTCCCA GAGAAGTGGT CCCTCGGCCC
    CGCCCTGGTG TCACGGAGGC CACCATTACT GGTCTGGAGC CAGGAACCGA GTACACCATC
    TACGTCATTG CCCTGAAGAA CAATCAGAAG AGTGAGCCCC TGATTGGGAG GAAGAAGACA
    GATGAGCTTC CCCAACTGGT TACCCTTCCA CACCCCAATC TTCATGGACC AGAGATCTTG
    GATGTTCCCT CCACAGTTCA AAAGACCCCC TTCATCACCA ACCCTGGGTA TGACACCGAA
    AATGGTATTC AGCTTCCTGG CACAACCCAC CAGCAACCCA GTGTTGGGCA ACAAATGATC
    TTTGAGGAAC ATGGCTTTAG GCGGACAACG CCACCCACTG CGGCCACCCC CGTCAGGCTT
    AGGCCAAGAC CATACCTGCC GAATGTAGAT GAGGAGGTCC AAATCGGTCA TGTTCCCAGG
    GGAGACGTAG ACTACCACCT CTATCCTCAT GTTCCGGGCC TCAATCCAAA TGCCTCTACA
    GGACAGGAAG CTCTCTCTCA GACAACCATC TCTTGGACGC CGTTCCAGGA GAGTTCTGAG
    TACATCATTT CATGCCAACC AGTTGGCACC GACGAAGAGC CCTTACAGTT CCAAGTTCCT
    GGAACTTCTA CCAGTGCGAC TCTGACTGGC CTTACCAGAG GGGTCACCTA CAACATCATA
    GTGGAGGCAC TGCAGAACCA GAGGAGGCAC AAGGTTCGGG AAGAGGTTGT GACTGTGGGC
    AACGCTGAAT GGTGCCATGA CAACGGTGTC AACTACAAGA TCGGAGAGAA GTGGGATCGG
    CAGGGAGAAA ATGGCCAGCG GATGAGCTGC ACATGCCTCG GGAATGGAAA GGGAGAATTC
    AAGTGTGATC CCCATGAAGC AACGTGCTAT GACGATGGGA AGACCTACCA TGTAGGAGAA
    CAGTGGCAGA AAGAATATCT CGGAGCCATT TGCTCCTGCA CGTGTTTCGG AGGCCAGCGG
    GGCTGGCGCT GTGACAACTG CCGTAGACCT GGGGCTGCTG AACCCAGTCC CGATGGCACC
    ACCGGCCACA CCTACAACCA GTATACACAG AGATACAATC AGAGAACAAA CACTAACGTA
    AATTGCCCCA TTGAGTGCTT CATGCCGCTA GATGTGCAAG CTGACAGAGA CGATTCTCGA
    GAGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu85006
    Clone ID Related Accession (Same CDS sequence) XM_030248098.1
    Accession Version XM_030248098.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7089bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product fibronectin isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    ATGCTCAGGG GTCCGGGACC CGGGCGGCTG CTGCTGCTGG CAGTCCTGTG CCTGGGGACC 
    TCGGTGCGCT GCACCGAAGC CGGGAAGAGC AAGAGGCAGG CTCAGCAAAT CGTGCAGCCT
    CAATCCCCGG TGGCTGTCAG TCAGAGCAAG CCTGGCTGTT TTGACAATGG GAAGCACTAT
    CAGATAAATC AGCAGTGGGA ACGGACCTAC CTAGGCAACG CCCTGGTTTG TACCTGCTAT
    GGAGGAAGCC GGGGTTTTAA CTGCGAGAGC AAGCCTGAGC CTGAAGAGAC TTGCTTTGAC
    AAATACACTG GGAACACTTA CAAAGTGGGT GACACTTATG AGCGCCCTAA AGATTCCATG
    ATCTGGGACT GTACCTGCAT CGGGGCTGGG AGAGGCAGGA TCAGCTGTAC CATTGCAAAT
    CGCTGCCATG AAGGGGGTCA GTCCTACAAG ATTGGCGACA AGTGGAGGAG GCCACATGAG
    ACTGGTGGCT ACATGTTAGA GTGTCTGTGT CTGGGAAATG GAAAAGGGGA ATGGACCTGC
    AAACCTATAG CTGAGAAGTG TTTTGATCAT GCTGCTGGGA CGTCCTACGT CGTGGGGGAG
    ACCTGGGAAA AGCCCTACCA AGGCTGGATG ATGGTGGACT GTACTTGTCT AGGCGAAGGC
    AATGGACGCA TCACCTGTAC CTCCAGAAAC AGATGCAACG ATCAGGACAC CCGGACATCC
    TATAGGATTG GAGACACGTG GAGCAAGAAG GACAACCGAG GAAACCTGCT TCAGTGTGTC
    TGCACAGGCA ATGGCAGAGG GGAGTGGAAG TGTGAGCGAC ATGCTCTACA AAGTGCTTCA
    GCCGGATCTG GCTCCTTCAC TGATGTCCGA ACAGCTATTT ACCAACCGCA GACTCACCCC
    CAGCCCGCTC CCTACGGCCA CTGTGTCACC GACAGTGGTG TGGTCTACTC TGTGGGAATG
    CAGTGGCTGA AGTCGCAAGG AAACAAGCAA ATGCTGTGCA CGTGCCTGGG CAATGGCGTC
    AGCTGCCAGG AGACAGCCGT GACCCAGACT TATGGTGGCA ATTCAAACGG GGAGCCCTGT
    GTCCTCCCGT TCACCTACAA CGGTAGGACC TTCTATTCCT GCACCACCGA AGGGCGGCAA
    GACGGACATC TGTGGTGTAG CACAACTTCC AATTACGAAC AAGACCAGAA GTATTCCTTC
    TGCACAGACC ATGCGGTTTT GGTTCAGACT CGAGGCGGAA ATTCCAATGG TGCTCTGTGC
    CACTTCCCCT TCCTGTACAA CAACCGGAAT TACACCGACT GTACTTCTGA GGGTCGCAGG
    GACAACATGA AATGGTGCGG CACCACCCAG AACTACGATG CCGATCAGAA GTTTGGATTC
    TGCCCAATGG CTGCCCACGA GGAGATCTGC ACAACCAATG AAGGGGTCAT GTATCGCATT
    GGGGATCAGT GGGATAAGCA GCATGACCTG GGCCACATGA TGAGGTGCAC GTGTGTGGGG
    AACGGTCGTG GAGAATGGGC CTGCATCCCC TACTCCCAGC TCCGAGACCA GTGCATCGTT
    GATGACATTA CTTACAATGT GAACGACACG TTCCACAAGC GTCACGAGGA GGGACATATG
    CTGAACTGTA CCTGCTTTGG TCAGGGCCGG GGCAGATGGA AGTGTGACCC CATTGACCAG
    TGCCAAGATT CAGAGACCCG GACATTTTAC CAGATTGGTG ACTCCTGGGA GAAGTTTGTG
    CATGGTGTCC GATACCAGTG TTACTGCTAC GGCCGTGGCA TCGGGGAGTG GCACTGTCAA
    CCTCTGCAGA CCTACCCAGG CACAACTGGA CCTGTCCAAG TAATTATCAC GGAGACCCCC
    AGCCAGCCCA ATTCCCACCC CATCCAGTGG AATGCCCCGG AGCCTTCACA CATCACCAAG
    TACATTCTCA GATGGAGACC TAAAACCTCT ACGGGTCGCT GGAAGGAAGC TACCATTCCT
    GGCCACCTTA ACTCCTATAC CATCAAAGGC CTGACCCCAG GTGTGATCTA TGAGGGACAG
    CTCATCAGCA TCCAGCAGTA TGGCCACAGA GAAGTGACAC GCTTCGACTT CACCACCAGC
    GCCAGCACCC CTGTGACCAG CAACACGGTG ACCGGAGAGA CTGCGCCCTA CTCTCCTGTT
    GTGGCCACTT CTGAATCTGT AACTGAAATC ACAGCCAGCA GCTTTGTGGT CTCATGGGTC
    TCAGCCTCCG ACACCGTGTC AGGCTTCCGG GTGGAGTATG AGCTGAGCGA GGAGGGAGAT
    GAACCACAGT ACCTTGATCT CCCAAGCACG GCCACTTCCG TGAACATTCC TGACCTGCTC
    CCGGGCAGAA AGTACATTGT CAATGTCTAT CAGATATCTG AGGAGGGAAA ACAGAGCTTG
    ATCCTGTCTA CCTCACAGAC TACAGCACCT GACGCTCCTC CAGACCCTAC CGTGGACCAG
    GTTGATGATA CTTCCATTGT TGTTCGGTGG AGTAGACCCC AGGCACCTAT CACAGGGTAT
    AGAATTGTCT ATTCACCTTC AGTAGAAGGC AGTAGCACAG AGCTCAACCT CCCTGAAACG
    GCCAACTCCG TCACCCTCAG CGACCTGCAG CCCGGTGTTC AGTACAACAT CACTATCTAT
    GCTGTGGAGG AGAACCAGGA GAGCACACCC GTTTTCATCC AACAAGAGAC CACTGGCACC
    CCAAGATCTG ATAACGTCCC CCCTCCGACG GACCTACAGT TTGTGGAACT GACTGATGTG
    AAAGTCACCA TCATGTGGAC CCCTCCTGAT AGTGTGGTGT CTGGATACCG TGTGGAGGTC
    CTGCCTGTCA GCCTGCCCGG GGAACATGGG CAGAGGCTGC CTGTCAACAG AAATACCTTT
    GCTGAAATCA CTGGGCTGTC CCCTGGGGTC ACGTACCTCT TCAAAGTCTT TGCTGTGCAC
    CAGGGCAGGG AAAGCAATCC TCTGACGGCA CAACAGACCA CCAAACTCGA CGCTCCCACT
    AACCTCCAGT TTGTCAATGA AACTGACAGA ACAGTTCTGG TAACGTGGAC TCCACCTCGA
    GCCCGTATAG CAGGCTACCG ACTGACCGCG GGCCTGACCC GAGGAGGCCA GCCCAAGCAG
    TACAATGTGG GACCCTTGGC CTCCAAGTAT CCCCTGAGAA ATCTGCAGCC TGGGTCTGAG
    TACACCGTGA CCTTGGTGGC TGTGAAAGGG AACCAGCAGA GTCCCAAAGC CACCGGAGTC
    TTTACTACCC TGCAGCCTCT GCGCTCCATT CCACCTTACA ACACCGAGGT GACAGAGACC
    ACAATTGTGA TCACCTGGAC CCCCGCTCCA AGGATTGGCT TCAAGCTGGG TGTACGACCG
    AGCCAGGGAG GTGAGGCACC CCGAGAAGTG ACTTCAGACT CTGGGAGCAT TGTTGTGTCT
    GGCTTGACTC CAGGCGTGGA ATACACTTAC ACCATCCAAG TCCTGCGAGA TGGCCAGGAG
    AGAGATGCAC CGATTGTCAA CAGAGTAGTG ACACCGCTGT CTCCACCGAC CAACTTGCAT
    CTGGAGGCAA ACCCTGACAC TGGAGTGCTT ACTGTCTCCT GGGAGAGGAG CACTACCCCA
    GATATCACTG GCTACAGAAT AACTACTACC CCCACGAACG GGCAGCAGGG GACCTCTCTG
    GAAGAAGTGG TCCATGCTGA TCAGAGTTCC TGCACTTTTG AGAACCTGAA TCCTGGCCTG
    GAGTACAACG TCAGTGTTTA CACTGTCAAA GATGACAAGG AAAGTGCCCC TATCTCTGAT
    ACCGTTGTCC CAGAGGTGCC CCAGCTCACT GACCTAAGCT TTGTTGATAT AACTGATTCA
    AGCATCGGCC TGAGGTGGAC CCCGCTAAAC TCTTCCACCA TTATCGGGTA CCGAATCACA
    GTAGTTGCGG CAGGAGAAGG GATCCCTATT TTTGAAGATT TTGTGGACTC CTCAGTAGGA
    TACTACACAG TTACAGGGCT GGAGCCTGGC ATTGACTATG ACATCAGCGT TATCACTCTC
    ATTAATGGCG GAGAGAGTGC CCCTACTACA CTGACACAGC AAACGGCTGT CCCTCCTCCC
    ACGGATCTGC GATTCACCAA TATCGGTCCA GACACGATGC GGGTCACTTG GGCCCCGCCT
    CCGTCCATCG AGCTGACCAA CCTCTTGGTG CGCTACTCAC CCGTGAAGAA TGAAGAGGAC
    GTTGCAGAGC TATCCATTTC ACCTTCAGAC AATGCCGTGG TCCTAACAAA TCTCCTGCCT
    GGGACAGAAT ACTTAGTCAG TGTCTCCAGT GTCTACGAAC AACATGAGAG CATCCCTCTC
    CGGGGAAGGC AGAAAACAGG TCTCGATTCC CCAACTGGTT TTGATTCTTC TGATATCACC
    GCCAACTCAT TCACTGTCCA CTGGGTGGCT CCTCGGGCCC CCATCACCGG CTACATCATC
    CGCCATCACG CCGAGCATTC TGTCGGAAGA CCCAGGCAGG ATCGAGTGCC GCCCTCGCGG
    AATTCCATCA CCCTCACCAA CCTTAATCCG GGCACCGAGT ACGTTGTCAG CATCATTGCT
    GTTAATGGCA GAGAGGAGAG CCCGCCACTG ATTGGCCAGC AAGCCACAGT TTCTGATATT
    CCGAGAGATC TGGAGGTCAT TGCCTCCACC CCCACCAGCC TGCTCATCAG TTGGGAACCC
    CCTGCCGTCT CTGTGCGCTA TTACAGAATC ACCTACGGAG AGACAGGAGG AAATAGCCCT
    GTCCAGGAGT TCACTGTGCC CGGAAGCAAG TCCACAGCCA CCATCAACAA CATTAAACCA
    GGAGCAGACT ACACCATCAC CCTGTATGCT GTCACTGGCC GTGGGGACAG TCCAGCAAGC
    AGCAAGCCAG TTTCCATCAA TTATAAAACA GAAATTGACA AGCCGTCCCA GATGCAGGTG
    ACAGATGTCC AGGACAACAG CATCAGTGTC AGGTGGCTGC CTTCAACTTC TCCTGTGACA
    GGCTACAGAG TGACCACCAC TCCCAAAAAT GGCCTAGGAC CATCAAAAAC TAAAACTGCC
    AGTCCAGATC AAACAGAAAT GACCATTGAA GGTTTGCAAC CCACTGTGGA GTACGTGGTT
    AGTGTTTATG CTCAGAACCG GAACGGAGAA AGCCAGCCCC TGGTTCAAAC TGCAGTGACC
    ACCATTCCTG CGCCCACCAA TCTGAAGTTC AGTCAGGTGA CACCCACCAG CTTTACTGCC
    CAGTGGATAG CACCCAGTGT TCAGCTCACT GGCTACCGGG TGCGGGTGAA CCCGAAAGAG
    AAGACAGGAC CAATGAAAGA AATCAACCTT TCTCCAGACA GCTCATCGGT GATTGTGTCA
    GGACTCATGG TGGCCACTAA ATACGAAGTC AGTGTCTATG CTCTCAAGGA CACACTGACA
    AGCAGACCAG CCCAGGGAGT CATCACTACT CTGGAGAATG TTAGCCCTCC AAGAAGGGCC
    CGTGTGACGG ACGCTACAGA GACCACCATC ACTATTAGCT GGAGAACAAA GACAGAGACA
    ATCACTGGCT TCCAAGTCGA TGCCATCCCA GCCAATGGCC AGACCCCAGT TCAGAGGAGC
    ATCAGCCCGG ATGTTAGAAG CTACACCATT ACAGGTTTAC AGCCAGGCAC TGACTACAAG
    ATCCACCTGT ACACTCTGAA CGACAATGCC CGGAGCTCGC CCGTGATCAT CGATGCCTCC
    ACCGCCATCG ACGCACCATC CAACCTGCGG TTCCTGACCA CCACACCCAA CTCCTTGCTG
    GTGTCATGGC AGGCGCCCCG TGCCAGGATT ACTGGCTACA TTATCAAGTA TGAGAAGCCT
    GGATCCCCTC CCAGAGAAGT GGTCCCTCGG CCCCGCCCTG GTGTCACGGA GGCCACCATT
    ACTGGTCTGG AGCCAGGAAC CGAGTACACC ATCTACGTCA TTGCCCTGAA GAACAATCAG
    AAGAGTGAGC CCCTGATTGG GAGGAAGAAG ACAGTTCAAA AGACCCCCTT CATCACCAAC
    CCTGGGTATG ACACCGAAAA TGGTATTCAG CTTCCTGGCA CAACCCACCA GCAACCCAGT
    GTTGGGCAAC AAATGATCTT TGAGGAACAT GGCTTTAGGC GGACAACGCC ACCCACTGCG
    GCCACCCCCG TCAGGCTTAG GCCAAGACCA TACCTGCCGA ATGTAGATGA GGAGGTCCAA
    ATCGGTCATG TTCCCAGGGG AGACGTAGAC TACCACCTCT ATCCTCATGT TCCGGGCCTC
    AATCCAAATG CCTCTACAGG ACAGGAAGCT CTCTCTCAGA CAACCATCTC TTGGACGCCG
    TTCCAGGAGA GTTCTGAGTA CATCATTTCA TGCCAACCAG TTGGCACCGA CGAAGAGCCC
    TTACAGTTCC AAGTTCCTGG AACTTCTACC AGTGCGACTC TGACTGGCCT TACCAGAGGG
    GTCACCTACA ACATCATAGT GGAGGCACTG CAGAACCAGA GGAGGCACAA GGTTCGGGAA
    GAGGTTGTGA CTGTGGGCAA CGCTGTCAGC GAAGGCCTGA ACCAGCCTAC AGATGACTCA
    TGCTTTGACC CTTACACGGT TTCCCATTAC GCCATTGGAG AGGAGTGGGA GCGGTTGTCT
    GACGCTGGCT TTAAGCTCAC ATGCCAGTGC TTGGGCTTTG GCAGTGGTCA TTTCAGATGC
    GATTCATCTA AATGGTGCCA TGACAACGGT GTCAACTACA AGATCGGAGA GAAGTGGGAT
    CGGCAGGGAG AAAATGGCCA GCGGATGAGC TGCACATGCC TCGGGAATGG AAAGGGAGAA
    TTCAAGTGTG ATCCCCATGA AGCAACGTGC TATGACGATG GGAAGACCTA CCATGTAGGA
    GAACAGTGGC AGAAAGAATA TCTCGGAGCC ATTTGCTCCT GCACGTGTTT CGGAGGCCAG
    CGGGGCTGGC GCTGTGACAA CTGCCGTAGA CCTGGGGCTG CTGAACCCAG TCCCGATGGC
    ACCACCGGCC ACACCTACAA CCAGTATACA CAGAGATACA ATCAGAGAAC AAACACTAAC
    GTAAATTGCC CCATTGAGTG CTTCATGCCG CTAGATGTGC AAGCTGACAG AGACGATTCT
    CGAGAGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_030103958.1
    CDS299..7387
    Translation

    Target ORF information:

    RefSeq Version XM_030248098.1
    Organism Mus musculus(house mouse)
    Definition Mus musculus fibronectin 1 (Fn1), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_030248098.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    ATGCTCAGGG GTCCGGGACC CGGGCGGCTG CTGCTGCTGG CAGTCCTGTG CCTGGGGACC 
    TCGGTGCGCT GCACCGAAGC CGGGAAGAGC AAGAGGCAGG CTCAGCAAAT CGTGCAGCCT
    CAATCCCCGG TGGCTGTCAG TCAGAGCAAG CCTGGCTGTT TTGACAATGG GAAGCACTAT
    CAGATAAATC AGCAGTGGGA ACGGACCTAC CTAGGCAACG CCCTGGTTTG TACCTGCTAT
    GGAGGAAGCC GGGGTTTTAA CTGCGAGAGC AAGCCTGAGC CTGAAGAGAC TTGCTTTGAC
    AAATACACTG GGAACACTTA CAAAGTGGGT GACACTTATG AGCGCCCTAA AGATTCCATG
    ATCTGGGACT GTACCTGCAT CGGGGCTGGG AGAGGCAGGA TCAGCTGTAC CATTGCAAAT
    CGCTGCCATG AAGGGGGTCA GTCCTACAAG ATTGGCGACA AGTGGAGGAG GCCACATGAG
    ACTGGTGGCT ACATGTTAGA GTGTCTGTGT CTGGGAAATG GAAAAGGGGA ATGGACCTGC
    AAACCTATAG CTGAGAAGTG TTTTGATCAT GCTGCTGGGA CGTCCTACGT CGTGGGGGAG
    ACCTGGGAAA AGCCCTACCA AGGCTGGATG ATGGTGGACT GTACTTGTCT AGGCGAAGGC
    AATGGACGCA TCACCTGTAC CTCCAGAAAC AGATGCAACG ATCAGGACAC CCGGACATCC
    TATAGGATTG GAGACACGTG GAGCAAGAAG GACAACCGAG GAAACCTGCT TCAGTGTGTC
    TGCACAGGCA ATGGCAGAGG GGAGTGGAAG TGTGAGCGAC ATGCTCTACA AAGTGCTTCA
    GCCGGATCTG GCTCCTTCAC TGATGTCCGA ACAGCTATTT ACCAACCGCA GACTCACCCC
    CAGCCCGCTC CCTACGGCCA CTGTGTCACC GACAGTGGTG TGGTCTACTC TGTGGGAATG
    CAGTGGCTGA AGTCGCAAGG AAACAAGCAA ATGCTGTGCA CGTGCCTGGG CAATGGCGTC
    AGCTGCCAGG AGACAGCCGT GACCCAGACT TATGGTGGCA ATTCAAACGG GGAGCCCTGT
    GTCCTCCCGT TCACCTACAA CGGTAGGACC TTCTATTCCT GCACCACCGA AGGGCGGCAA
    GACGGACATC TGTGGTGTAG CACAACTTCC AATTACGAAC AAGACCAGAA GTATTCCTTC
    TGCACAGACC ATGCGGTTTT GGTTCAGACT CGAGGCGGAA ATTCCAATGG TGCTCTGTGC
    CACTTCCCCT TCCTGTACAA CAACCGGAAT TACACCGACT GTACTTCTGA GGGTCGCAGG
    GACAACATGA AATGGTGCGG CACCACCCAG AACTACGATG CCGATCAGAA GTTTGGATTC
    TGCCCAATGG CTGCCCACGA GGAGATCTGC ACAACCAATG AAGGGGTCAT GTATCGCATT
    GGGGATCAGT GGGATAAGCA GCATGACCTG GGCCACATGA TGAGGTGCAC GTGTGTGGGG
    AACGGTCGTG GAGAATGGGC CTGCATCCCC TACTCCCAGC TCCGAGACCA GTGCATCGTT
    GATGACATTA CTTACAATGT GAACGACACG TTCCACAAGC GTCACGAGGA GGGACATATG
    CTGAACTGTA CCTGCTTTGG TCAGGGCCGG GGCAGATGGA AGTGTGACCC CATTGACCAG
    TGCCAAGATT CAGAGACCCG GACATTTTAC CAGATTGGTG ACTCCTGGGA GAAGTTTGTG
    CATGGTGTCC GATACCAGTG TTACTGCTAC GGCCGTGGCA TCGGGGAGTG GCACTGTCAA
    CCTCTGCAGA CCTACCCAGG CACAACTGGA CCTGTCCAAG TAATTATCAC GGAGACCCCC
    AGCCAGCCCA ATTCCCACCC CATCCAGTGG AATGCCCCGG AGCCTTCACA CATCACCAAG
    TACATTCTCA GATGGAGACC TAAAACCTCT ACGGGTCGCT GGAAGGAAGC TACCATTCCT
    GGCCACCTTA ACTCCTATAC CATCAAAGGC CTGACCCCAG GTGTGATCTA TGAGGGACAG
    CTCATCAGCA TCCAGCAGTA TGGCCACAGA GAAGTGACAC GCTTCGACTT CACCACCAGC
    GCCAGCACCC CTGTGACCAG CAACACGGTG ACCGGAGAGA CTGCGCCCTA CTCTCCTGTT
    GTGGCCACTT CTGAATCTGT AACTGAAATC ACAGCCAGCA GCTTTGTGGT CTCATGGGTC
    TCAGCCTCCG ACACCGTGTC AGGCTTCCGG GTGGAGTATG AGCTGAGCGA GGAGGGAGAT
    GAACCACAGT ACCTTGATCT CCCAAGCACG GCCACTTCCG TGAACATTCC TGACCTGCTC
    CCGGGCAGAA AGTACATTGT CAATGTCTAT CAGATATCTG AGGAGGGAAA ACAGAGCTTG
    ATCCTGTCTA CCTCACAGAC TACAGCACCT GACGCTCCTC CAGACCCTAC CGTGGACCAG
    GTTGATGATA CTTCCATTGT TGTTCGGTGG AGTAGACCCC AGGCACCTAT CACAGGGTAT
    AGAATTGTCT ATTCACCTTC AGTAGAAGGC AGTAGCACAG AGCTCAACCT CCCTGAAACG
    GCCAACTCCG TCACCCTCAG CGACCTGCAG CCCGGTGTTC AGTACAACAT CACTATCTAT
    GCTGTGGAGG AGAACCAGGA GAGCACACCC GTTTTCATCC AACAAGAGAC CACTGGCACC
    CCAAGATCTG ATAACGTCCC CCCTCCGACG GACCTACAGT TTGTGGAACT GACTGATGTG
    AAAGTCACCA TCATGTGGAC CCCTCCTGAT AGTGTGGTGT CTGGATACCG TGTGGAGGTC
    CTGCCTGTCA GCCTGCCCGG GGAACATGGG CAGAGGCTGC CTGTCAACAG AAATACCTTT
    GCTGAAATCA CTGGGCTGTC CCCTGGGGTC ACGTACCTCT TCAAAGTCTT TGCTGTGCAC
    CAGGGCAGGG AAAGCAATCC TCTGACGGCA CAACAGACCA CCAAACTCGA CGCTCCCACT
    AACCTCCAGT TTGTCAATGA AACTGACAGA ACAGTTCTGG TAACGTGGAC TCCACCTCGA
    GCCCGTATAG CAGGCTACCG ACTGACCGCG GGCCTGACCC GAGGAGGCCA GCCCAAGCAG
    TACAATGTGG GACCCTTGGC CTCCAAGTAT CCCCTGAGAA ATCTGCAGCC TGGGTCTGAG
    TACACCGTGA CCTTGGTGGC TGTGAAAGGG AACCAGCAGA GTCCCAAAGC CACCGGAGTC
    TTTACTACCC TGCAGCCTCT GCGCTCCATT CCACCTTACA ACACCGAGGT GACAGAGACC
    ACAATTGTGA TCACCTGGAC CCCCGCTCCA AGGATTGGCT TCAAGCTGGG TGTACGACCG
    AGCCAGGGAG GTGAGGCACC CCGAGAAGTG ACTTCAGACT CTGGGAGCAT TGTTGTGTCT
    GGCTTGACTC CAGGCGTGGA ATACACTTAC ACCATCCAAG TCCTGCGAGA TGGCCAGGAG
    AGAGATGCAC CGATTGTCAA CAGAGTAGTG ACACCGCTGT CTCCACCGAC CAACTTGCAT
    CTGGAGGCAA ACCCTGACAC TGGAGTGCTT ACTGTCTCCT GGGAGAGGAG CACTACCCCA
    GATATCACTG GCTACAGAAT AACTACTACC CCCACGAACG GGCAGCAGGG GACCTCTCTG
    GAAGAAGTGG TCCATGCTGA TCAGAGTTCC TGCACTTTTG AGAACCTGAA TCCTGGCCTG
    GAGTACAACG TCAGTGTTTA CACTGTCAAA GATGACAAGG AAAGTGCCCC TATCTCTGAT
    ACCGTTGTCC CAGAGGTGCC CCAGCTCACT GACCTAAGCT TTGTTGATAT AACTGATTCA
    AGCATCGGCC TGAGGTGGAC CCCGCTAAAC TCTTCCACCA TTATCGGGTA CCGAATCACA
    GTAGTTGCGG CAGGAGAAGG GATCCCTATT TTTGAAGATT TTGTGGACTC CTCAGTAGGA
    TACTACACAG TTACAGGGCT GGAGCCTGGC ATTGACTATG ACATCAGCGT TATCACTCTC
    ATTAATGGCG GAGAGAGTGC CCCTACTACA CTGACACAGC AAACGGCTGT CCCTCCTCCC
    ACGGATCTGC GATTCACCAA TATCGGTCCA GACACGATGC GGGTCACTTG GGCCCCGCCT
    CCGTCCATCG AGCTGACCAA CCTCTTGGTG CGCTACTCAC CCGTGAAGAA TGAAGAGGAC
    GTTGCAGAGC TATCCATTTC ACCTTCAGAC AATGCCGTGG TCCTAACAAA TCTCCTGCCT
    GGGACAGAAT ACTTAGTCAG TGTCTCCAGT GTCTACGAAC AACATGAGAG CATCCCTCTC
    CGGGGAAGGC AGAAAACAGG TCTCGATTCC CCAACTGGTT TTGATTCTTC TGATATCACC
    GCCAACTCAT TCACTGTCCA CTGGGTGGCT CCTCGGGCCC CCATCACCGG CTACATCATC
    CGCCATCACG CCGAGCATTC TGTCGGAAGA CCCAGGCAGG ATCGAGTGCC GCCCTCGCGG
    AATTCCATCA CCCTCACCAA CCTTAATCCG GGCACCGAGT ACGTTGTCAG CATCATTGCT
    GTTAATGGCA GAGAGGAGAG CCCGCCACTG ATTGGCCAGC AAGCCACAGT TTCTGATATT
    CCGAGAGATC TGGAGGTCAT TGCCTCCACC CCCACCAGCC TGCTCATCAG TTGGGAACCC
    CCTGCCGTCT CTGTGCGCTA TTACAGAATC ACCTACGGAG AGACAGGAGG AAATAGCCCT
    GTCCAGGAGT TCACTGTGCC CGGAAGCAAG TCCACAGCCA CCATCAACAA CATTAAACCA
    GGAGCAGACT ACACCATCAC CCTGTATGCT GTCACTGGCC GTGGGGACAG TCCAGCAAGC
    AGCAAGCCAG TTTCCATCAA TTATAAAACA GAAATTGACA AGCCGTCCCA GATGCAGGTG
    ACAGATGTCC AGGACAACAG CATCAGTGTC AGGTGGCTGC CTTCAACTTC TCCTGTGACA
    GGCTACAGAG TGACCACCAC TCCCAAAAAT GGCCTAGGAC CATCAAAAAC TAAAACTGCC
    AGTCCAGATC AAACAGAAAT GACCATTGAA GGTTTGCAAC CCACTGTGGA GTACGTGGTT
    AGTGTTTATG CTCAGAACCG GAACGGAGAA AGCCAGCCCC TGGTTCAAAC TGCAGTGACC
    ACCATTCCTG CGCCCACCAA TCTGAAGTTC AGTCAGGTGA CACCCACCAG CTTTACTGCC
    CAGTGGATAG CACCCAGTGT TCAGCTCACT GGCTACCGGG TGCGGGTGAA CCCGAAAGAG
    AAGACAGGAC CAATGAAAGA AATCAACCTT TCTCCAGACA GCTCATCGGT GATTGTGTCA
    GGACTCATGG TGGCCACTAA ATACGAAGTC AGTGTCTATG CTCTCAAGGA CACACTGACA
    AGCAGACCAG CCCAGGGAGT CATCACTACT CTGGAGAATG TTAGCCCTCC AAGAAGGGCC
    CGTGTGACGG ACGCTACAGA GACCACCATC ACTATTAGCT GGAGAACAAA GACAGAGACA
    ATCACTGGCT TCCAAGTCGA TGCCATCCCA GCCAATGGCC AGACCCCAGT TCAGAGGAGC
    ATCAGCCCGG ATGTTAGAAG CTACACCATT ACAGGTTTAC AGCCAGGCAC TGACTACAAG
    ATCCACCTGT ACACTCTGAA CGACAATGCC CGGAGCTCGC CCGTGATCAT CGATGCCTCC
    ACCGCCATCG ACGCACCATC CAACCTGCGG TTCCTGACCA CCACACCCAA CTCCTTGCTG
    GTGTCATGGC AGGCGCCCCG TGCCAGGATT ACTGGCTACA TTATCAAGTA TGAGAAGCCT
    GGATCCCCTC CCAGAGAAGT GGTCCCTCGG CCCCGCCCTG GTGTCACGGA GGCCACCATT
    ACTGGTCTGG AGCCAGGAAC CGAGTACACC ATCTACGTCA TTGCCCTGAA GAACAATCAG
    AAGAGTGAGC CCCTGATTGG GAGGAAGAAG ACAGTTCAAA AGACCCCCTT CATCACCAAC
    CCTGGGTATG ACACCGAAAA TGGTATTCAG CTTCCTGGCA CAACCCACCA GCAACCCAGT
    GTTGGGCAAC AAATGATCTT TGAGGAACAT GGCTTTAGGC GGACAACGCC ACCCACTGCG
    GCCACCCCCG TCAGGCTTAG GCCAAGACCA TACCTGCCGA ATGTAGATGA GGAGGTCCAA
    ATCGGTCATG TTCCCAGGGG AGACGTAGAC TACCACCTCT ATCCTCATGT TCCGGGCCTC
    AATCCAAATG CCTCTACAGG ACAGGAAGCT CTCTCTCAGA CAACCATCTC TTGGACGCCG
    TTCCAGGAGA GTTCTGAGTA CATCATTTCA TGCCAACCAG TTGGCACCGA CGAAGAGCCC
    TTACAGTTCC AAGTTCCTGG AACTTCTACC AGTGCGACTC TGACTGGCCT TACCAGAGGG
    GTCACCTACA ACATCATAGT GGAGGCACTG CAGAACCAGA GGAGGCACAA GGTTCGGGAA
    GAGGTTGTGA CTGTGGGCAA CGCTGTCAGC GAAGGCCTGA ACCAGCCTAC AGATGACTCA
    TGCTTTGACC CTTACACGGT TTCCCATTAC GCCATTGGAG AGGAGTGGGA GCGGTTGTCT
    GACGCTGGCT TTAAGCTCAC ATGCCAGTGC TTGGGCTTTG GCAGTGGTCA TTTCAGATGC
    GATTCATCTA AATGGTGCCA TGACAACGGT GTCAACTACA AGATCGGAGA GAAGTGGGAT
    CGGCAGGGAG AAAATGGCCA GCGGATGAGC TGCACATGCC TCGGGAATGG AAAGGGAGAA
    TTCAAGTGTG ATCCCCATGA AGCAACGTGC TATGACGATG GGAAGACCTA CCATGTAGGA
    GAACAGTGGC AGAAAGAATA TCTCGGAGCC ATTTGCTCCT GCACGTGTTT CGGAGGCCAG
    CGGGGCTGGC GCTGTGACAA CTGCCGTAGA CCTGGGGCTG CTGAACCCAG TCCCGATGGC
    ACCACCGGCC ACACCTACAA CCAGTATACA CAGAGATACA ATCAGAGAAC AAACACTAAC
    GTAAATTGCC CCATTGAGTG CTTCATGCCG CTAGATGTGC AAGCTGACAG AGACGATTCT
    CGAGAGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu85007
    Clone ID Related Accession (Same CDS sequence) XM_030248135.1
    Accession Version XM_030248135.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6909bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product fibronectin isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    ATGCTCAGGG GTCCGGGACC CGGGCGGCTG CTGCTGCTGG CAGTCCTGTG CCTGGGGACC 
    TCGGTGCGCT GCACCGAAGC CGGGAAGAGC AAGAGGCAGG CTCAGCAAAT CGTGCAGCCT
    CAATCCCCGG TGGCTGTCAG TCAGAGCAAG CCTGGCTGTT TTGACAATGG GAAGCACTAT
    CAGATAAATC AGCAGTGGGA ACGGACCTAC CTAGGCAACG CCCTGGTTTG TACCTGCTAT
    GGAGGAAGCC GGGGTTTTAA CTGCGAGAGC AAGCCTGAGC CTGAAGAGAC TTGCTTTGAC
    AAATACACTG GGAACACTTA CAAAGTGGGT GACACTTATG AGCGCCCTAA AGATTCCATG
    ATCTGGGACT GTACCTGCAT CGGGGCTGGG AGAGGCAGGA TCAGCTGTAC CATTGCAAAT
    CGCTGCCATG AAGGGGGTCA GTCCTACAAG ATTGGCGACA AGTGGAGGAG GCCACATGAG
    ACTGGTGGCT ACATGTTAGA GTGTCTGTGT CTGGGAAATG GAAAAGGGGA ATGGACCTGC
    AAACCTATAG CTGAGAAGTG TTTTGATCAT GCTGCTGGGA CGTCCTACGT CGTGGGGGAG
    ACCTGGGAAA AGCCCTACCA AGGCTGGATG ATGGTGGACT GTACTTGTCT AGGCGAAGGC
    AATGGACGCA TCACCTGTAC CTCCAGAAAC AGATGCAACG ATCAGGACAC CCGGACATCC
    TATAGGATTG GAGACACGTG GAGCAAGAAG GACAACCGAG GAAACCTGCT TCAGTGTGTC
    TGCACAGGCA ATGGCAGAGG GGAGTGGAAG TGTGAGCGAC ATGCTCTACA AAGTGCTTCA
    GCCGGATCTG GCTCCTTCAC TGATGTCCGA ACAGCTATTT ACCAACCGCA GACTCACCCC
    CAGCCCGCTC CCTACGGCCA CTGTGTCACC GACAGTGGTG TGGTCTACTC TGTGGGAATG
    CAGTGGCTGA AGTCGCAAGG AAACAAGCAA ATGCTGTGCA CGTGCCTGGG CAATGGCGTC
    AGCTGCCAGG AGACAGCCGT GACCCAGACT TATGGTGGCA ATTCAAACGG GGAGCCCTGT
    GTCCTCCCGT TCACCTACAA CGGTAGGACC TTCTATTCCT GCACCACCGA AGGGCGGCAA
    GACGGACATC TGTGGTGTAG CACAACTTCC AATTACGAAC AAGACCAGAA GTATTCCTTC
    TGCACAGACC ATGCGGTTTT GGTTCAGACT CGAGGCGGAA ATTCCAATGG TGCTCTGTGC
    CACTTCCCCT TCCTGTACAA CAACCGGAAT TACACCGACT GTACTTCTGA GGGTCGCAGG
    GACAACATGA AATGGTGCGG CACCACCCAG AACTACGATG CCGATCAGAA GTTTGGATTC
    TGCCCAATGG CTGCCCACGA GGAGATCTGC ACAACCAATG AAGGGGTCAT GTATCGCATT
    GGGGATCAGT GGGATAAGCA GCATGACCTG GGCCACATGA TGAGGTGCAC GTGTGTGGGG
    AACGGTCGTG GAGAATGGGC CTGCATCCCC TACTCCCAGC TCCGAGACCA GTGCATCGTT
    GATGACATTA CTTACAATGT GAACGACACG TTCCACAAGC GTCACGAGGA GGGACATATG
    CTGAACTGTA CCTGCTTTGG TCAGGGCCGG GGCAGATGGA AGTGTGACCC CATTGACCAG
    TGCCAAGATT CAGAGACCCG GACATTTTAC CAGATTGGTG ACTCCTGGGA GAAGTTTGTG
    CATGGTGTCC GATACCAGTG TTACTGCTAC GGCCGTGGCA TCGGGGAGTG GCACTGTCAA
    CCTCTGCAGA CCTACCCAGG CACAACTGGA CCTGTCCAAG TAATTATCAC GGAGACCCCC
    AGCCAGCCCA ATTCCCACCC CATCCAGTGG AATGCCCCGG AGCCTTCACA CATCACCAAG
    TACATTCTCA GATGGAGACC TAAAACCTCT ACGGGTCGCT GGAAGGAAGC TACCATTCCT
    GGCCACCTTA ACTCCTATAC CATCAAAGGC CTGACCCCAG GTGTGATCTA TGAGGGACAG
    CTCATCAGCA TCCAGCAGTA TGGCCACAGA GAAGTGACAC GCTTCGACTT CACCACCAGC
    GCCAGCACCC CTGTGACCAG CAACACGGTG ACCGGAGAGA CTGCGCCCTA CTCTCCTGTT
    GTGGCCACTT CTGAATCTGT AACTGAAATC ACAGCCAGCA GCTTTGTGGT CTCATGGGTC
    TCAGCCTCCG ACACCGTGTC AGGCTTCCGG GTGGAGTATG AGCTGAGCGA GGAGGGAGAT
    GAACCACAGT ACCTTGATCT CCCAAGCACG GCCACTTCCG TGAACATTCC TGACCTGCTC
    CCGGGCAGAA AGTACATTGT CAATGTCTAT CAGATATCTG AGGAGGGAAA ACAGAGCTTG
    ATCCTGTCTA CCTCACAGAC TACAGCACCT GACGCTCCTC CAGACCCTAC CGTGGACCAG
    GTTGATGATA CTTCCATTGT TGTTCGGTGG AGTAGACCCC AGGCACCTAT CACAGGGTAT
    AGAATTGTCT ATTCACCTTC AGTAGAAGGC AGTAGCACAG AGCTCAACCT CCCTGAAACG
    GCCAACTCCG TCACCCTCAG CGACCTGCAG CCCGGTGTTC AGTACAACAT CACTATCTAT
    GCTGTGGAGG AGAACCAGGA GAGCACACCC GTTTTCATCC AACAAGAGAC CACTGGCACC
    CCAAGATCTG ATAACGTCCC CCCTCCGACG GACCTACAGT TTGTGGAACT GACTGATGTG
    AAAGTCACCA TCATGTGGAC CCCTCCTGAT AGTGTGGTGT CTGGATACCG TGTGGAGGTC
    CTGCCTGTCA GCCTGCCCGG GGAACATGGG CAGAGGCTGC CTGTCAACAG AAATACCTTT
    GCTGAAATCA CTGGGCTGTC CCCTGGGGTC ACGTACCTCT TCAAAGTCTT TGCTGTGCAC
    CAGGGCAGGG AAAGCAATCC TCTGACGGCA CAACAGACCA CCAAACTCGA CGCTCCCACT
    AACCTCCAGT TTGTCAATGA AACTGACAGA ACAGTTCTGG TAACGTGGAC TCCACCTCGA
    GCCCGTATAG CAGGCTACCG ACTGACCGCG GGCCTGACCC GAGGAGGCCA GCCCAAGCAG
    TACAATGTGG GACCCTTGGC CTCCAAGTAT CCCCTGAGAA ATCTGCAGCC TGGGTCTGAG
    TACACCGTGA CCTTGGTGGC TGTGAAAGGG AACCAGCAGA GTCCCAAAGC CACCGGAGTC
    TTTACTACCC TGCAGCCTCT GCGCTCCATT CCACCTTACA ACACCGAGGT GACAGAGACC
    ACAATTGTGA TCACCTGGAC CCCCGCTCCA AGGATTGGCT TCAAGCTGGG TGTACGACCG
    AGCCAGGGAG GTGAGGCACC CCGAGAAGTG ACTTCAGACT CTGGGAGCAT TGTTGTGTCT
    GGCTTGACTC CAGGCGTGGA ATACACTTAC ACCATCCAAG TCCTGCGAGA TGGCCAGGAG
    AGAGATGCAC CGATTGTCAA CAGAGTAGTG ACACCGCTGT CTCCACCGAC CAACTTGCAT
    CTGGAGGCAA ACCCTGACAC TGGAGTGCTT ACTGTCTCCT GGGAGAGGAG CACTACCCCA
    GATATCACTG GCTACAGAAT AACTACTACC CCCACGAACG GGCAGCAGGG GACCTCTCTG
    GAAGAAGTGG TCCATGCTGA TCAGAGTTCC TGCACTTTTG AGAACCTGAA TCCTGGCCTG
    GAGTACAACG TCAGTGTTTA CACTGTCAAA GATGACAAGG AAAGTGCCCC TATCTCTGAT
    ACCGTTGTCC CAGAGGTGCC CCAGCTCACT GACCTAAGCT TTGTTGATAT AACTGATTCA
    AGCATCGGCC TGAGGTGGAC CCCGCTAAAC TCTTCCACCA TTATCGGGTA CCGAATCACA
    GTAGTTGCGG CAGGAGAAGG GATCCCTATT TTTGAAGATT TTGTGGACTC CTCAGTAGGA
    TACTACACAG TTACAGGGCT GGAGCCTGGC ATTGACTATG ACATCAGCGT TATCACTCTC
    ATTAATGGCG GAGAGAGTGC CCCTACTACA CTGACACAGC AAACGGCTGT CCCTCCTCCC
    ACGGATCTGC GATTCACCAA TATCGGTCCA GACACGATGC GGGTCACTTG GGCCCCGCCT
    CCGTCCATCG AGCTGACCAA CCTCTTGGTG CGCTACTCAC CCGTGAAGAA TGAAGAGGAC
    GTTGCAGAGC TATCCATTTC ACCTTCAGAC AATGCCGTGG TCCTAACAAA TCTCCTGCCT
    GGGACAGAAT ACTTAGTCAG TGTCTCCAGT GTCTACGAAC AACATGAGAG CATCCCTCTC
    CGGGGAAGGC AGAAAACAGG TCTCGATTCC CCAACTGGTT TTGATTCTTC TGATATCACC
    GCCAACTCAT TCACTGTCCA CTGGGTGGCT CCTCGGGCCC CCATCACCGG CTACATCATC
    CGCCATCACG CCGAGCATTC TGTCGGAAGA CCCAGGCAGG ATCGAGTGCC GCCCTCGCGG
    AATTCCATCA CCCTCACCAA CCTTAATCCG GGCACCGAGT ACGTTGTCAG CATCATTGCT
    GTTAATGGCA GAGAGGAGAG CCCGCCACTG ATTGGCCAGC AAGCCACAGT TTCTGATATT
    CCGAGAGATC TGGAGGTCAT TGCCTCCACC CCCACCAGCC TGCTCATCAG TTGGGAACCC
    CCTGCCGTCT CTGTGCGCTA TTACAGAATC ACCTACGGAG AGACAGGAGG AAATAGCCCT
    GTCCAGGAGT TCACTGTGCC CGGAAGCAAG TCCACAGCCA CCATCAACAA CATTAAACCA
    GGAGCAGACT ACACCATCAC CCTGTATGCT GTCACTGGCC GTGGGGACAG TCCAGCAAGC
    AGCAAGCCAG TTTCCATCAA TTATAAAACA GAAATTGACA AGCCGTCCCA GATGCAGGTG
    ACAGATGTCC AGGACAACAG CATCAGTGTC AGGTGGCTGC CTTCAACTTC TCCTGTGACA
    GGCTACAGAG TGACCACCAC TCCCAAAAAT GGCCTAGGAC CATCAAAAAC TAAAACTGCC
    AGTCCAGATC AAACAGAAAT GACCATTGAA GGTTTGCAAC CCACTGTGGA GTACGTGGTT
    AGTGTTTATG CTCAGAACCG GAACGGAGAA AGCCAGCCCC TGGTTCAAAC TGCAGTGACC
    AACATTGATC GCCCTAAAGG ACTGGCATTC ACTGATGTGG ATGTCGATTC CATCAAAATT
    GCTTGGGAAA GCCCACAGGG GCAAGTTTCC AGGTACAGGG TGACCTACTC GAGCCCTGAG
    GATGGAATCC GGGAGCTTTT CCCTGCACCT GATGGTGAAG ACGACACTGC AGAGCTGCAG
    GGCCTCAGGC CGGGGTCTGA GTACACAGTC AGTGTGGTTG CCTTGCACGA TGATATGGAG
    AGCCAGCCCC TGATTGGAAT CCAGTCCACA GCCATTCCTG CGCCCACCAA TCTGAAGTTC
    AGTCAGGTGA CACCCACCAG CTTTACTGCC CAGTGGATAG CACCCAGTGT TCAGCTCACT
    GGCTACCGGG TGCGGGTGAA CCCGAAAGAG AAGACAGGAC CAATGAAAGA AATCAACCTT
    TCTCCAGACA GCTCATCGGT GATTGTGTCA GGACTCATGG TGGCCACTAA ATACGAAGTC
    AGTGTCTATG CTCTCAAGGA CACACTGACA AGCAGACCAG CCCAGGGAGT CATCACTACT
    CTGGAGAATG TTAGCCCTCC AAGAAGGGCC CGTGTGACGG ACGCTACAGA GACCACCATC
    ACTATTAGCT GGAGAACAAA GACAGAGACA ATCACTGGCT TCCAAGTCGA TGCCATCCCA
    GCCAATGGCC AGACCCCAGT TCAGAGGAGC ATCAGCCCGG ATGTTAGAAG CTACACCATT
    ACAGGTTTAC AGCCAGGCAC TGACTACAAG ATCCACCTGT ACACTCTGAA CGACAATGCC
    CGGAGCTCGC CCGTGATCAT CGATGCCTCC ACCGCCATCG ACGCACCATC CAACCTGCGG
    TTCCTGACCA CCACACCCAA CTCCTTGCTG GTGTCATGGC AGGCGCCCCG TGCCAGGATT
    ACTGGCTACA TTATCAAGTA TGAGAAGCCT GGATCCCCTC CCAGAGAAGT GGTCCCTCGG
    CCCCGCCCTG GTGTCACGGA GGCCACCATT ACTGGTCTGG AGCCAGGAAC CGAGTACACC
    ATCTACGTCA TTGCCCTGAA GAACAATCAG AAGAGTGAGC CCCTGATTGG GAGGAAGAAG
    ACAGGACAGG AAGCTCTCTC TCAGACAACC ATCTCTTGGA CGCCGTTCCA GGAGAGTTCT
    GAGTACATCA TTTCATGCCA ACCAGTTGGC ACCGACGAAG AGCCCTTACA GTTCCAAGTT
    CCTGGAACTT CTACCAGTGC GACTCTGACT GGCCTTACCA GAGGGGTCAC CTACAACATC
    ATAGTGGAGG CACTGCAGAA CCAGAGGAGG CACAAGGTTC GGGAAGAGGT TGTGACTGTG
    GGCAACGCTG AATGGTGCCA TGACAACGGT GTCAACTACA AGATCGGAGA GAAGTGGGAT
    CGGCAGGGAG AAAATGGCCA GCGGATGAGC TGCACATGCC TCGGGAATGG AAAGGGAGAA
    TTCAAGTGTG ATCCCCATGA AGCAACGTGC TATGACGATG GGAAGACCTA CCATGTAGGA
    GAACAGTGGC AGAAAGAATA TCTCGGAGCC ATTTGCTCCT GCACGTGTTT CGGAGGCCAG
    CGGGGCTGGC GCTGTGACAA CTGCCGTAGA CCTGGGGCTG CTGAACCCAG TCCCGATGGC
    ACCACCGGCC ACACCTACAA CCAGTATACA CAGAGATACA ATCAGAGAAC AAACACTAAC
    GTAAATTGCC CCATTGAGTG CTTCATGCCG CTAGATGTGC AAGCTGACAG AGACGATTCT
    CGAGAGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_030103995.1
    CDS299..7207
    Translation

    Target ORF information:

    RefSeq Version XM_030248135.1
    Organism Mus musculus(house mouse)
    Definition Mus musculus fibronectin 1 (Fn1), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_030248135.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    ATGCTCAGGG GTCCGGGACC CGGGCGGCTG CTGCTGCTGG CAGTCCTGTG CCTGGGGACC 
    TCGGTGCGCT GCACCGAAGC CGGGAAGAGC AAGAGGCAGG CTCAGCAAAT CGTGCAGCCT
    CAATCCCCGG TGGCTGTCAG TCAGAGCAAG CCTGGCTGTT TTGACAATGG GAAGCACTAT
    CAGATAAATC AGCAGTGGGA ACGGACCTAC CTAGGCAACG CCCTGGTTTG TACCTGCTAT
    GGAGGAAGCC GGGGTTTTAA CTGCGAGAGC AAGCCTGAGC CTGAAGAGAC TTGCTTTGAC
    AAATACACTG GGAACACTTA CAAAGTGGGT GACACTTATG AGCGCCCTAA AGATTCCATG
    ATCTGGGACT GTACCTGCAT CGGGGCTGGG AGAGGCAGGA TCAGCTGTAC CATTGCAAAT
    CGCTGCCATG AAGGGGGTCA GTCCTACAAG ATTGGCGACA AGTGGAGGAG GCCACATGAG
    ACTGGTGGCT ACATGTTAGA GTGTCTGTGT CTGGGAAATG GAAAAGGGGA ATGGACCTGC
    AAACCTATAG CTGAGAAGTG TTTTGATCAT GCTGCTGGGA CGTCCTACGT CGTGGGGGAG
    ACCTGGGAAA AGCCCTACCA AGGCTGGATG ATGGTGGACT GTACTTGTCT AGGCGAAGGC
    AATGGACGCA TCACCTGTAC CTCCAGAAAC AGATGCAACG ATCAGGACAC CCGGACATCC
    TATAGGATTG GAGACACGTG GAGCAAGAAG GACAACCGAG GAAACCTGCT TCAGTGTGTC
    TGCACAGGCA ATGGCAGAGG GGAGTGGAAG TGTGAGCGAC ATGCTCTACA AAGTGCTTCA
    GCCGGATCTG GCTCCTTCAC TGATGTCCGA ACAGCTATTT ACCAACCGCA GACTCACCCC
    CAGCCCGCTC CCTACGGCCA CTGTGTCACC GACAGTGGTG TGGTCTACTC TGTGGGAATG
    CAGTGGCTGA AGTCGCAAGG AAACAAGCAA ATGCTGTGCA CGTGCCTGGG CAATGGCGTC
    AGCTGCCAGG AGACAGCCGT GACCCAGACT TATGGTGGCA ATTCAAACGG GGAGCCCTGT
    GTCCTCCCGT TCACCTACAA CGGTAGGACC TTCTATTCCT GCACCACCGA AGGGCGGCAA
    GACGGACATC TGTGGTGTAG CACAACTTCC AATTACGAAC AAGACCAGAA GTATTCCTTC
    TGCACAGACC ATGCGGTTTT GGTTCAGACT CGAGGCGGAA ATTCCAATGG TGCTCTGTGC
    CACTTCCCCT TCCTGTACAA CAACCGGAAT TACACCGACT GTACTTCTGA GGGTCGCAGG
    GACAACATGA AATGGTGCGG CACCACCCAG AACTACGATG CCGATCAGAA GTTTGGATTC
    TGCCCAATGG CTGCCCACGA GGAGATCTGC ACAACCAATG AAGGGGTCAT GTATCGCATT
    GGGGATCAGT GGGATAAGCA GCATGACCTG GGCCACATGA TGAGGTGCAC GTGTGTGGGG
    AACGGTCGTG GAGAATGGGC CTGCATCCCC TACTCCCAGC TCCGAGACCA GTGCATCGTT
    GATGACATTA CTTACAATGT GAACGACACG TTCCACAAGC GTCACGAGGA GGGACATATG
    CTGAACTGTA CCTGCTTTGG TCAGGGCCGG GGCAGATGGA AGTGTGACCC CATTGACCAG
    TGCCAAGATT CAGAGACCCG GACATTTTAC CAGATTGGTG ACTCCTGGGA GAAGTTTGTG
    CATGGTGTCC GATACCAGTG TTACTGCTAC GGCCGTGGCA TCGGGGAGTG GCACTGTCAA
    CCTCTGCAGA CCTACCCAGG CACAACTGGA CCTGTCCAAG TAATTATCAC GGAGACCCCC
    AGCCAGCCCA ATTCCCACCC CATCCAGTGG AATGCCCCGG AGCCTTCACA CATCACCAAG
    TACATTCTCA GATGGAGACC TAAAACCTCT ACGGGTCGCT GGAAGGAAGC TACCATTCCT
    GGCCACCTTA ACTCCTATAC CATCAAAGGC CTGACCCCAG GTGTGATCTA TGAGGGACAG
    CTCATCAGCA TCCAGCAGTA TGGCCACAGA GAAGTGACAC GCTTCGACTT CACCACCAGC
    GCCAGCACCC CTGTGACCAG CAACACGGTG ACCGGAGAGA CTGCGCCCTA CTCTCCTGTT
    GTGGCCACTT CTGAATCTGT AACTGAAATC ACAGCCAGCA GCTTTGTGGT CTCATGGGTC
    TCAGCCTCCG ACACCGTGTC AGGCTTCCGG GTGGAGTATG AGCTGAGCGA GGAGGGAGAT
    GAACCACAGT ACCTTGATCT CCCAAGCACG GCCACTTCCG TGAACATTCC TGACCTGCTC
    CCGGGCAGAA AGTACATTGT CAATGTCTAT CAGATATCTG AGGAGGGAAA ACAGAGCTTG
    ATCCTGTCTA CCTCACAGAC TACAGCACCT GACGCTCCTC CAGACCCTAC CGTGGACCAG
    GTTGATGATA CTTCCATTGT TGTTCGGTGG AGTAGACCCC AGGCACCTAT CACAGGGTAT
    AGAATTGTCT ATTCACCTTC AGTAGAAGGC AGTAGCACAG AGCTCAACCT CCCTGAAACG
    GCCAACTCCG TCACCCTCAG CGACCTGCAG CCCGGTGTTC AGTACAACAT CACTATCTAT
    GCTGTGGAGG AGAACCAGGA GAGCACACCC GTTTTCATCC AACAAGAGAC CACTGGCACC
    CCAAGATCTG ATAACGTCCC CCCTCCGACG GACCTACAGT TTGTGGAACT GACTGATGTG
    AAAGTCACCA TCATGTGGAC CCCTCCTGAT AGTGTGGTGT CTGGATACCG TGTGGAGGTC
    CTGCCTGTCA GCCTGCCCGG GGAACATGGG CAGAGGCTGC CTGTCAACAG AAATACCTTT
    GCTGAAATCA CTGGGCTGTC CCCTGGGGTC ACGTACCTCT TCAAAGTCTT TGCTGTGCAC
    CAGGGCAGGG AAAGCAATCC TCTGACGGCA CAACAGACCA CCAAACTCGA CGCTCCCACT
    AACCTCCAGT TTGTCAATGA AACTGACAGA ACAGTTCTGG TAACGTGGAC TCCACCTCGA
    GCCCGTATAG CAGGCTACCG ACTGACCGCG GGCCTGACCC GAGGAGGCCA GCCCAAGCAG
    TACAATGTGG GACCCTTGGC CTCCAAGTAT CCCCTGAGAA ATCTGCAGCC TGGGTCTGAG
    TACACCGTGA CCTTGGTGGC TGTGAAAGGG AACCAGCAGA GTCCCAAAGC CACCGGAGTC
    TTTACTACCC TGCAGCCTCT GCGCTCCATT CCACCTTACA ACACCGAGGT GACAGAGACC
    ACAATTGTGA TCACCTGGAC CCCCGCTCCA AGGATTGGCT TCAAGCTGGG TGTACGACCG
    AGCCAGGGAG GTGAGGCACC CCGAGAAGTG ACTTCAGACT CTGGGAGCAT TGTTGTGTCT
    GGCTTGACTC CAGGCGTGGA ATACACTTAC ACCATCCAAG TCCTGCGAGA TGGCCAGGAG
    AGAGATGCAC CGATTGTCAA CAGAGTAGTG ACACCGCTGT CTCCACCGAC CAACTTGCAT
    CTGGAGGCAA ACCCTGACAC TGGAGTGCTT ACTGTCTCCT GGGAGAGGAG CACTACCCCA
    GATATCACTG GCTACAGAAT AACTACTACC CCCACGAACG GGCAGCAGGG GACCTCTCTG
    GAAGAAGTGG TCCATGCTGA TCAGAGTTCC TGCACTTTTG AGAACCTGAA TCCTGGCCTG
    GAGTACAACG TCAGTGTTTA CACTGTCAAA GATGACAAGG AAAGTGCCCC TATCTCTGAT
    ACCGTTGTCC CAGAGGTGCC CCAGCTCACT GACCTAAGCT TTGTTGATAT AACTGATTCA
    AGCATCGGCC TGAGGTGGAC CCCGCTAAAC TCTTCCACCA TTATCGGGTA CCGAATCACA
    GTAGTTGCGG CAGGAGAAGG GATCCCTATT TTTGAAGATT TTGTGGACTC CTCAGTAGGA
    TACTACACAG TTACAGGGCT GGAGCCTGGC ATTGACTATG ACATCAGCGT TATCACTCTC
    ATTAATGGCG GAGAGAGTGC CCCTACTACA CTGACACAGC AAACGGCTGT CCCTCCTCCC
    ACGGATCTGC GATTCACCAA TATCGGTCCA GACACGATGC GGGTCACTTG GGCCCCGCCT
    CCGTCCATCG AGCTGACCAA CCTCTTGGTG CGCTACTCAC CCGTGAAGAA TGAAGAGGAC
    GTTGCAGAGC TATCCATTTC ACCTTCAGAC AATGCCGTGG TCCTAACAAA TCTCCTGCCT
    GGGACAGAAT ACTTAGTCAG TGTCTCCAGT GTCTACGAAC AACATGAGAG CATCCCTCTC
    CGGGGAAGGC AGAAAACAGG TCTCGATTCC CCAACTGGTT TTGATTCTTC TGATATCACC
    GCCAACTCAT TCACTGTCCA CTGGGTGGCT CCTCGGGCCC CCATCACCGG CTACATCATC
    CGCCATCACG CCGAGCATTC TGTCGGAAGA CCCAGGCAGG ATCGAGTGCC GCCCTCGCGG
    AATTCCATCA CCCTCACCAA CCTTAATCCG GGCACCGAGT ACGTTGTCAG CATCATTGCT
    GTTAATGGCA GAGAGGAGAG CCCGCCACTG ATTGGCCAGC AAGCCACAGT TTCTGATATT
    CCGAGAGATC TGGAGGTCAT TGCCTCCACC CCCACCAGCC TGCTCATCAG TTGGGAACCC
    CCTGCCGTCT CTGTGCGCTA TTACAGAATC ACCTACGGAG AGACAGGAGG AAATAGCCCT
    GTCCAGGAGT TCACTGTGCC CGGAAGCAAG TCCACAGCCA CCATCAACAA CATTAAACCA
    GGAGCAGACT ACACCATCAC CCTGTATGCT GTCACTGGCC GTGGGGACAG TCCAGCAAGC
    AGCAAGCCAG TTTCCATCAA TTATAAAACA GAAATTGACA AGCCGTCCCA GATGCAGGTG
    ACAGATGTCC AGGACAACAG CATCAGTGTC AGGTGGCTGC CTTCAACTTC TCCTGTGACA
    GGCTACAGAG TGACCACCAC TCCCAAAAAT GGCCTAGGAC CATCAAAAAC TAAAACTGCC
    AGTCCAGATC AAACAGAAAT GACCATTGAA GGTTTGCAAC CCACTGTGGA GTACGTGGTT
    AGTGTTTATG CTCAGAACCG GAACGGAGAA AGCCAGCCCC TGGTTCAAAC TGCAGTGACC
    AACATTGATC GCCCTAAAGG ACTGGCATTC ACTGATGTGG ATGTCGATTC CATCAAAATT
    GCTTGGGAAA GCCCACAGGG GCAAGTTTCC AGGTACAGGG TGACCTACTC GAGCCCTGAG
    GATGGAATCC GGGAGCTTTT CCCTGCACCT GATGGTGAAG ACGACACTGC AGAGCTGCAG
    GGCCTCAGGC CGGGGTCTGA GTACACAGTC AGTGTGGTTG CCTTGCACGA TGATATGGAG
    AGCCAGCCCC TGATTGGAAT CCAGTCCACA GCCATTCCTG CGCCCACCAA TCTGAAGTTC
    AGTCAGGTGA CACCCACCAG CTTTACTGCC CAGTGGATAG CACCCAGTGT TCAGCTCACT
    GGCTACCGGG TGCGGGTGAA CCCGAAAGAG AAGACAGGAC CAATGAAAGA AATCAACCTT
    TCTCCAGACA GCTCATCGGT GATTGTGTCA GGACTCATGG TGGCCACTAA ATACGAAGTC
    AGTGTCTATG CTCTCAAGGA CACACTGACA AGCAGACCAG CCCAGGGAGT CATCACTACT
    CTGGAGAATG TTAGCCCTCC AAGAAGGGCC CGTGTGACGG ACGCTACAGA GACCACCATC
    ACTATTAGCT GGAGAACAAA GACAGAGACA ATCACTGGCT TCCAAGTCGA TGCCATCCCA
    GCCAATGGCC AGACCCCAGT TCAGAGGAGC ATCAGCCCGG ATGTTAGAAG CTACACCATT
    ACAGGTTTAC AGCCAGGCAC TGACTACAAG ATCCACCTGT ACACTCTGAA CGACAATGCC
    CGGAGCTCGC CCGTGATCAT CGATGCCTCC ACCGCCATCG ACGCACCATC CAACCTGCGG
    TTCCTGACCA CCACACCCAA CTCCTTGCTG GTGTCATGGC AGGCGCCCCG TGCCAGGATT
    ACTGGCTACA TTATCAAGTA TGAGAAGCCT GGATCCCCTC CCAGAGAAGT GGTCCCTCGG
    CCCCGCCCTG GTGTCACGGA GGCCACCATT ACTGGTCTGG AGCCAGGAAC CGAGTACACC
    ATCTACGTCA TTGCCCTGAA GAACAATCAG AAGAGTGAGC CCCTGATTGG GAGGAAGAAG
    ACAGGACAGG AAGCTCTCTC TCAGACAACC ATCTCTTGGA CGCCGTTCCA GGAGAGTTCT
    GAGTACATCA TTTCATGCCA ACCAGTTGGC ACCGACGAAG AGCCCTTACA GTTCCAAGTT
    CCTGGAACTT CTACCAGTGC GACTCTGACT GGCCTTACCA GAGGGGTCAC CTACAACATC
    ATAGTGGAGG CACTGCAGAA CCAGAGGAGG CACAAGGTTC GGGAAGAGGT TGTGACTGTG
    GGCAACGCTG AATGGTGCCA TGACAACGGT GTCAACTACA AGATCGGAGA GAAGTGGGAT
    CGGCAGGGAG AAAATGGCCA GCGGATGAGC TGCACATGCC TCGGGAATGG AAAGGGAGAA
    TTCAAGTGTG ATCCCCATGA AGCAACGTGC TATGACGATG GGAAGACCTA CCATGTAGGA
    GAACAGTGGC AGAAAGAATA TCTCGGAGCC ATTTGCTCCT GCACGTGTTT CGGAGGCCAG
    CGGGGCTGGC GCTGTGACAA CTGCCGTAGA CCTGGGGCTG CTGAACCCAG TCCCGATGGC
    ACCACCGGCC ACACCTACAA CCAGTATACA CAGAGATACA ATCAGAGAAC AAACACTAAC
    GTAAATTGCC CCATTGAGTG CTTCATGCCG CTAGATGTGC AAGCTGACAG AGACGATTCT
    CGAGAGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu17493
    Clone ID Related Accession (Same CDS sequence) NM_001276409.1
    Accession Version NM_001276409.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7086bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-24
    Organism Mus musculus(house mouse)
    Product fibronectin isoform c precursor
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from CJ098561.1, AK147315.1 and AC124821.5. Transcript Variant: This variant (3) lacks an in-frame exon and uses an alternate in-frame splice site in the coding region, compared to variant 1. The encoded isoform (c) is shorter than isoform a. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK147315.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164134, SAMN01164142 [ECO:0000348] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    ATGCTCAGGG GTCCGGGACC CGGGCGGCTG CTGCTGCTGG CAGTCCTGTG CCTGGGGACC 
    TCGGTGCGCT GCACCGAAGC CGGGAAGAGC AAGAGGCAGG CTCAGCAAAT CGTGCAGCCT
    CAATCCCCGG TGGCTGTCAG TCAGAGCAAG CCTGGCTGTT TTGACAATGG GAAGCACTAT
    CAGATAAATC AGCAGTGGGA ACGGACCTAC CTAGGCAACG CCCTGGTTTG TACCTGCTAT
    GGAGGAAGCC GGGGTTTTAA CTGCGAGAGC AAGCCTGAGC CTGAAGAGAC TTGCTTTGAC
    AAATACACTG GGAACACTTA CAAAGTGGGT GACACTTATG AGCGCCCTAA AGATTCCATG
    ATCTGGGACT GTACCTGCAT CGGGGCTGGG AGAGGCAGGA TCAGCTGTAC CATTGCAAAT
    CGCTGCCATG AAGGGGGTCA GTCCTACAAG ATTGGCGACA AGTGGAGGAG GCCACATGAG
    ACTGGTGGCT ACATGTTAGA GTGTCTGTGT CTGGGAAATG GAAAAGGGGA ATGGACCTGC
    AAACCTATAG CTGAGAAGTG TTTTGATCAT GCTGCTGGGA CGTCCTACGT CGTGGGGGAG
    ACCTGGGAAA AGCCCTACCA AGGCTGGATG ATGGTGGACT GTACTTGTCT AGGCGAAGGC
    AATGGACGCA TCACCTGTAC CTCCAGAAAC AGATGCAACG ATCAGGACAC CCGGACATCC
    TATAGGATTG GAGACACGTG GAGCAAGAAG GACAACCGAG GAAACCTGCT TCAGTGTGTC
    TGCACAGGCA ATGGCAGAGG GGAGTGGAAG TGTGAGCGAC ATGCTCTACA AAGTGCTTCA
    GCCGGATCTG GCTCCTTCAC TGATGTCCGA ACAGCTATTT ACCAACCGCA GACTCACCCC
    CAGCCCGCTC CCTACGGCCA CTGTGTCACC GACAGTGGTG TGGTCTACTC TGTGGGAATG
    CAGTGGCTGA AGTCGCAAGG AAACAAGCAA ATGCTGTGCA CGTGCCTGGG CAATGGCGTC
    AGCTGCCAGG AGACAGCCGT GACCCAGACT TATGGTGGCA ATTCAAACGG GGAGCCCTGT
    GTCCTCCCGT TCACCTACAA CGGTAGGACC TTCTATTCCT GCACCACCGA AGGGCGGCAA
    GACGGACATC TGTGGTGTAG CACAACTTCC AATTACGAAC AAGACCAGAA GTATTCCTTC
    TGCACAGACC ATGCGGTTTT GGTTCAGACT CGAGGCGGAA ATTCCAATGG TGCTCTGTGC
    CACTTCCCCT TCCTGTACAA CAACCGGAAT TACACCGACT GTACTTCTGA GGGTCGCAGG
    GACAACATGA AATGGTGCGG CACCACCCAG AACTACGATG CCGATCAGAA GTTTGGATTC
    TGCCCAATGG CTGCCCACGA GGAGATCTGC ACAACCAATG AAGGGGTCAT GTATCGCATT
    GGGGATCAGT GGGATAAGCA GCATGACCTG GGCCACATGA TGAGGTGCAC GTGTGTGGGG
    AACGGTCGTG GAGAATGGGC CTGCATCCCC TACTCCCAGC TCCGAGACCA GTGCATCGTT
    GATGACATTA CTTACAATGT GAACGACACG TTCCACAAGC GTCACGAGGA GGGACATATG
    CTGAACTGTA CCTGCTTTGG TCAGGGCCGG GGCAGATGGA AGTGTGACCC CATTGACCAG
    TGCCAAGATT CAGAGACCCG GACATTTTAC CAGATTGGTG ACTCCTGGGA GAAGTTTGTG
    CATGGTGTCC GATACCAGTG TTACTGCTAC GGCCGTGGCA TCGGGGAGTG GCACTGTCAA
    CCTCTGCAGA CCTACCCAGG CACAACTGGA CCTGTCCAAG TAATTATCAC GGAGACCCCC
    AGCCAGCCCA ATTCCCACCC CATCCAGTGG AATGCCCCGG AGCCTTCACA CATCACCAAG
    TACATTCTCA GATGGAGACC TAAAACCTCT ACGGGTCGCT GGAAGGAAGC TACCATTCCT
    GGCCACCTTA ACTCCTATAC CATCAAAGGC CTGACCCCAG GTGTGATCTA TGAGGGACAG
    CTCATCAGCA TCCAGCAGTA TGGCCACAGA GAAGTGACAC GCTTCGACTT CACCACCAGC
    GCCAGCACCC CTGTGACCAG CAACACGGTG ACCGGAGAGA CTGCGCCCTA CTCTCCTGTT
    GTGGCCACTT CTGAATCTGT AACTGAAATC ACAGCCAGCA GCTTTGTGGT CTCATGGGTC
    TCAGCCTCCG ACACCGTGTC AGGCTTCCGG GTGGAGTATG AGCTGAGCGA GGAGGGAGAT
    GAACCACAGT ACCTTGATCT CCCAAGCACG GCCACTTCCG TGAACATTCC TGACCTGCTC
    CCGGGCAGAA AGTACATTGT CAATGTCTAT CAGATATCTG AGGAGGGAAA ACAGAGCTTG
    ATCCTGTCTA CCTCACAGAC TACAGCACCT GACGCTCCTC CAGACCCTAC CGTGGACCAG
    GTTGATGATA CTTCCATTGT TGTTCGGTGG AGTAGACCCC AGGCACCTAT CACAGGGTAT
    AGAATTGTCT ATTCACCTTC AGTAGAAGGC AGTAGCACAG AGCTCAACCT CCCTGAAACG
    GCCAACTCCG TCACCCTCAG CGACCTGCAG CCCGGTGTTC AGTACAACAT CACTATCTAT
    GCTGTGGAGG AGAACCAGGA GAGCACACCC GTTTTCATCC AACAAGAGAC CACTGGCACC
    CCAAGATCTG ATAACGTCCC CCCTCCGACG GACCTACAGT TTGTGGAACT GACTGATGTG
    AAAGTCACCA TCATGTGGAC CCCTCCTGAT AGTGTGGTGT CTGGATACCG TGTGGAGGTC
    CTGCCTGTCA GCCTGCCCGG GGAACATGGG CAGAGGCTGC CTGTCAACAG AAATACCTTT
    GCTGAAATCA CTGGGCTGTC CCCTGGGGTC ACGTACCTCT TCAAAGTCTT TGCTGTGCAC
    CAGGGCAGGG AAAGCAATCC TCTGACGGCA CAACAGACCA CCAAACTCGA CGCTCCCACT
    AACCTCCAGT TTGTCAATGA AACTGACAGA ACAGTTCTGG TAACGTGGAC TCCACCTCGA
    GCCCGTATAG CAGGCTACCG ACTGACCGCG GGCCTGACCC GAGGAGGCCA GCCCAAGCAG
    TACAATGTGG GACCCTTGGC CTCCAAGTAT CCCCTGAGAA ATCTGCAGCC TGGGTCTGAG
    TACACCGTGA CCTTGGTGGC TGTGAAAGGG AACCAGCAGA GTCCCAAAGC CACCGGAGTC
    TTTACTACCC TGCAGCCTCT GCGCTCCATT CCACCTTACA ACACCGAGGT GACAGAGACC
    ACAATTGTGA TCACCTGGAC CCCCGCTCCA AGGATTGGCT TCAAGCTGGG TGTACGACCG
    AGCCAGGGAG GTGAGGCACC CCGAGAAGTG ACTTCAGACT CTGGGAGCAT TGTTGTGTCT
    GGCTTGACTC CAGGCGTGGA ATACACTTAC ACCATCCAAG TCCTGCGAGA TGGCCAGGAG
    AGAGATGCAC CGATTGTCAA CAGAGTAGTG ACACCGCTGT CTCCACCGAC CAACTTGCAT
    CTGGAGGCAA ACCCTGACAC TGGAGTGCTT ACTGTCTCCT GGGAGAGGAG CACTACCCCA
    GATATCACTG GCTACAGAAT AACTACTACC CCCACGAACG GGCAGCAGGG GACCTCTCTG
    GAAGAAGTGG TCCATGCTGA TCAGAGTTCC TGCACTTTTG AGAACCTGAA TCCTGGCCTG
    GAGTACAACG TCAGTGTTTA CACTGTCAAA GATGACAAGG AAAGTGCCCC TATCTCTGAT
    ACCGTTGTCC CAGCTGTCCC TCCTCCCACG GATCTGCGAT TCACCAATAT CGGTCCAGAC
    ACGATGCGGG TCACTTGGGC CCCGCCTCCG TCCATCGAGC TGACCAACCT CTTGGTGCGC
    TACTCACCCG TGAAGAATGA AGAGGACGTT GCAGAGCTAT CCATTTCACC TTCAGACAAT
    GCCGTGGTCC TAACAAATCT CCTGCCTGGG ACAGAATACT TAGTCAGTGT CTCCAGTGTC
    TACGAACAAC ATGAGAGCAT CCCTCTCCGG GGAAGGCAGA AAACAGGTCT CGATTCCCCA
    ACTGGTTTTG ATTCTTCTGA TATCACCGCC AACTCATTCA CTGTCCACTG GGTGGCTCCT
    CGGGCCCCCA TCACCGGCTA CATCATCCGC CATCACGCCG AGCATTCTGT CGGAAGACCC
    AGGCAGGATC GAGTGCCGCC CTCGCGGAAT TCCATCACCC TCACCAACCT TAATCCGGGC
    ACCGAGTACG TTGTCAGCAT CATTGCTGTT AATGGCAGAG AGGAGAGCCC GCCACTGATT
    GGCCAGCAAG CCACAGTTTC TGATATTCCG AGAGATCTGG AGGTCATTGC CTCCACCCCC
    ACCAGCCTGC TCATCAGTTG GGAACCCCCT GCCGTCTCTG TGCGCTATTA CAGAATCACC
    TACGGAGAGA CAGGAGGAAA TAGCCCTGTC CAGGAGTTCA CTGTGCCCGG AAGCAAGTCC
    ACAGCCACCA TCAACAACAT TAAACCAGGA GCAGACTACA CCATCACCCT GTATGCTGTC
    ACTGGCCGTG GGGACAGTCC AGCAAGCAGC AAGCCAGTTT CCATCAATTA TAAAACAGAA
    ATTGACAAGC CGTCCCAGAT GCAGGTGACA GATGTCCAGG ACAACAGCAT CAGTGTCAGG
    TGGCTGCCTT CAACTTCTCC TGTGACAGGC TACAGAGTGA CCACCACTCC CAAAAATGGC
    CTAGGACCAT CAAAAACTAA AACTGCCAGT CCAGATCAAA CAGAAATGAC CATTGAAGGT
    TTGCAACCCA CTGTGGAGTA CGTGGTTAGT GTTTATGCTC AGAACCGGAA CGGAGAAAGC
    CAGCCCCTGG TTCAAACTGC AGTGACCAAC ATTGATCGCC CTAAAGGACT GGCATTCACT
    GATGTGGATG TCGATTCCAT CAAAATTGCT TGGGAAAGCC CACAGGGGCA AGTTTCCAGG
    TACAGGGTGA CCTACTCGAG CCCTGAGGAT GGAATCCGGG AGCTTTTCCC TGCACCTGAT
    GGTGAAGACG ACACTGCAGA GCTGCAGGGC CTCAGGCCGG GGTCTGAGTA CACAGTCAGT
    GTGGTTGCCT TGCACGATGA TATGGAGAGC CAGCCCCTGA TTGGAATCCA GTCCACAGCC
    ATTCCTGCGC CCACCAATCT GAAGTTCAGT CAGGTGACAC CCACCAGCTT TACTGCCCAG
    TGGATAGCAC CCAGTGTTCA GCTCACTGGC TACCGGGTGC GGGTGAACCC GAAAGAGAAG
    ACAGGACCAA TGAAAGAAAT CAACCTTTCT CCAGACAGCT CATCGGTGAT TGTGTCAGGA
    CTCATGGTGG CCACTAAATA CGAAGTCAGT GTCTATGCTC TCAAGGACAC ACTGACAAGC
    AGACCAGCCC AGGGAGTCAT CACTACTCTG GAGAATGTTA GCCCTCCAAG AAGGGCCCGT
    GTGACGGACG CTACAGAGAC CACCATCACT ATTAGCTGGA GAACAAAGAC AGAGACAATC
    ACTGGCTTCC AAGTCGATGC CATCCCAGCC AATGGCCAGA CCCCAGTTCA GAGGAGCATC
    AGCCCGGATG TTAGAAGCTA CACCATTACA GGTTTACAGC CAGGCACTGA CTACAAGATC
    CACCTGTACA CTCTGAACGA CAATGCCCGG AGCTCGCCCG TGATCATCGA TGCCTCCACC
    GCCATCGACG CACCATCCAA CCTGCGGTTC CTGACCACCA CACCCAACTC CTTGCTGGTG
    TCATGGCAGG CGCCCCGTGC CAGGATTACT GGCTACATTA TCAAGTATGA GAAGCCTGGA
    TCCCCTCCCA GAGAAGTGGT CCCTCGGCCC CGCCCTGGTG TCACGGAGGC CACCATTACT
    GGTCTGGAGC CAGGAACCGA GTACACCATC TACGTCATTG CCCTGAAGAA CAATCAGAAG
    AGTGAGCCCC TGATTGGGAG GAAGAAGACA GTTCAAAAGA CCCCCTTCAT CACCAACCCT
    GGGTATGACA CCGAAAATGG TATTCAGCTT CCTGGCACAA CCCACCAGCA ACCCAGTGTT
    GGGCAACAAA TGATCTTTGA GGAACATGGC TTTAGGCGGA CAACGCCACC CACTGCGGCC
    ACCCCCGTCA GGCTTAGGCC AAGACCATAC CTGCCGAATG TAGATGAGGA GGTCCAAATC
    GGTCATGTTC CCAGGGGAGA CGTAGACTAC CACCTCTATC CTCATGTTCC GGGCCTCAAT
    CCAAATGCCT CTACAGGACA GGAAGCTCTC TCTCAGACAA CCATCTCTTG GACGCCGTTC
    CAGGAGAGTT CTGAGTACAT CATTTCATGC CAACCAGTTG GCACCGACGA AGAGCCCTTA
    CAGTTCCAAG TTCCTGGAAC TTCTACCAGT GCGACTCTGA CTGGCCTTAC CAGAGGGGTC
    ACCTACAACA TCATAGTGGA GGCACTGCAG AACCAGAGGA GGCACAAGGT TCGGGAAGAG
    GTTGTGACTG TGGGCAACGC TGTCAGCGAA GGCCTGAACC AGCCTACAGA TGACTCATGC
    TTTGACCCTT ACACGGTTTC CCATTACGCC ATTGGAGAGG AGTGGGAGCG GTTGTCTGAC
    GCTGGCTTTA AGCTCACATG CCAGTGCTTG GGCTTTGGCA GTGGTCATTT CAGATGCGAT
    TCATCTAAAT GGTGCCATGA CAACGGTGTC AACTACAAGA TCGGAGAGAA GTGGGATCGG
    CAGGGAGAAA ATGGCCAGCG GATGAGCTGC ACATGCCTCG GGAATGGAAA GGGAGAATTC
    AAGTGTGATC CCCATGAAGC AACGTGCTAT GACGATGGGA AGACCTACCA TGTAGGAGAA
    CAGTGGCAGA AAGAATATCT CGGAGCCATT TGCTCCTGCA CGTGTTTCGG AGGCCAGCGG
    GGCTGGCGCT GTGACAACTG CCGTAGACCT GGGGCTGCTG AACCCAGTCC CGATGGCACC
    ACCGGCCACA CCTACAACCA GTATACACAG AGATACAATC AGAGAACAAA CACTAACGTA
    AATTGCCCCA TTGAGTGCTT CATGCCGCTA GATGTGCAAG CTGACAGAGA CGATTCTCGA
    GAGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001263338.1
    CDS253..7338
    Translation

    Target ORF information:

    RefSeq Version NM_001276409.1
    Organism Mus musculus(house mouse)
    Definition Mus musculus fibronectin 1 (Fn1), transcript variant 3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001276409.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    ATGCTCAGGG GTCCGGGACC CGGGCGGCTG CTGCTGCTGG CAGTCCTGTG CCTGGGGACC 
    TCGGTGCGCT GCACCGAAGC CGGGAAGAGC AAGAGGCAGG CTCAGCAAAT CGTGCAGCCT
    CAATCCCCGG TGGCTGTCAG TCAGAGCAAG CCTGGCTGTT TTGACAATGG GAAGCACTAT
    CAGATAAATC AGCAGTGGGA ACGGACCTAC CTAGGCAACG CCCTGGTTTG TACCTGCTAT
    GGAGGAAGCC GGGGTTTTAA CTGCGAGAGC AAGCCTGAGC CTGAAGAGAC TTGCTTTGAC
    AAATACACTG GGAACACTTA CAAAGTGGGT GACACTTATG AGCGCCCTAA AGATTCCATG
    ATCTGGGACT GTACCTGCAT CGGGGCTGGG AGAGGCAGGA TCAGCTGTAC CATTGCAAAT
    CGCTGCCATG AAGGGGGTCA GTCCTACAAG ATTGGCGACA AGTGGAGGAG GCCACATGAG
    ACTGGTGGCT ACATGTTAGA GTGTCTGTGT CTGGGAAATG GAAAAGGGGA ATGGACCTGC
    AAACCTATAG CTGAGAAGTG TTTTGATCAT GCTGCTGGGA CGTCCTACGT CGTGGGGGAG
    ACCTGGGAAA AGCCCTACCA AGGCTGGATG ATGGTGGACT GTACTTGTCT AGGCGAAGGC
    AATGGACGCA TCACCTGTAC CTCCAGAAAC AGATGCAACG ATCAGGACAC CCGGACATCC
    TATAGGATTG GAGACACGTG GAGCAAGAAG GACAACCGAG GAAACCTGCT TCAGTGTGTC
    TGCACAGGCA ATGGCAGAGG GGAGTGGAAG TGTGAGCGAC ATGCTCTACA AAGTGCTTCA
    GCCGGATCTG GCTCCTTCAC TGATGTCCGA ACAGCTATTT ACCAACCGCA GACTCACCCC
    CAGCCCGCTC CCTACGGCCA CTGTGTCACC GACAGTGGTG TGGTCTACTC TGTGGGAATG
    CAGTGGCTGA AGTCGCAAGG AAACAAGCAA ATGCTGTGCA CGTGCCTGGG CAATGGCGTC
    AGCTGCCAGG AGACAGCCGT GACCCAGACT TATGGTGGCA ATTCAAACGG GGAGCCCTGT
    GTCCTCCCGT TCACCTACAA CGGTAGGACC TTCTATTCCT GCACCACCGA AGGGCGGCAA
    GACGGACATC TGTGGTGTAG CACAACTTCC AATTACGAAC AAGACCAGAA GTATTCCTTC
    TGCACAGACC ATGCGGTTTT GGTTCAGACT CGAGGCGGAA ATTCCAATGG TGCTCTGTGC
    CACTTCCCCT TCCTGTACAA CAACCGGAAT TACACCGACT GTACTTCTGA GGGTCGCAGG
    GACAACATGA AATGGTGCGG CACCACCCAG AACTACGATG CCGATCAGAA GTTTGGATTC
    TGCCCAATGG CTGCCCACGA GGAGATCTGC ACAACCAATG AAGGGGTCAT GTATCGCATT
    GGGGATCAGT GGGATAAGCA GCATGACCTG GGCCACATGA TGAGGTGCAC GTGTGTGGGG
    AACGGTCGTG GAGAATGGGC CTGCATCCCC TACTCCCAGC TCCGAGACCA GTGCATCGTT
    GATGACATTA CTTACAATGT GAACGACACG TTCCACAAGC GTCACGAGGA GGGACATATG
    CTGAACTGTA CCTGCTTTGG TCAGGGCCGG GGCAGATGGA AGTGTGACCC CATTGACCAG
    TGCCAAGATT CAGAGACCCG GACATTTTAC CAGATTGGTG ACTCCTGGGA GAAGTTTGTG
    CATGGTGTCC GATACCAGTG TTACTGCTAC GGCCGTGGCA TCGGGGAGTG GCACTGTCAA
    CCTCTGCAGA CCTACCCAGG CACAACTGGA CCTGTCCAAG TAATTATCAC GGAGACCCCC
    AGCCAGCCCA ATTCCCACCC CATCCAGTGG AATGCCCCGG AGCCTTCACA CATCACCAAG
    TACATTCTCA GATGGAGACC TAAAACCTCT ACGGGTCGCT GGAAGGAAGC TACCATTCCT
    GGCCACCTTA ACTCCTATAC CATCAAAGGC CTGACCCCAG GTGTGATCTA TGAGGGACAG
    CTCATCAGCA TCCAGCAGTA TGGCCACAGA GAAGTGACAC GCTTCGACTT CACCACCAGC
    GCCAGCACCC CTGTGACCAG CAACACGGTG ACCGGAGAGA CTGCGCCCTA CTCTCCTGTT
    GTGGCCACTT CTGAATCTGT AACTGAAATC ACAGCCAGCA GCTTTGTGGT CTCATGGGTC
    TCAGCCTCCG ACACCGTGTC AGGCTTCCGG GTGGAGTATG AGCTGAGCGA GGAGGGAGAT
    GAACCACAGT ACCTTGATCT CCCAAGCACG GCCACTTCCG TGAACATTCC TGACCTGCTC
    CCGGGCAGAA AGTACATTGT CAATGTCTAT CAGATATCTG AGGAGGGAAA ACAGAGCTTG
    ATCCTGTCTA CCTCACAGAC TACAGCACCT GACGCTCCTC CAGACCCTAC CGTGGACCAG
    GTTGATGATA CTTCCATTGT TGTTCGGTGG AGTAGACCCC AGGCACCTAT CACAGGGTAT
    AGAATTGTCT ATTCACCTTC AGTAGAAGGC AGTAGCACAG AGCTCAACCT CCCTGAAACG
    GCCAACTCCG TCACCCTCAG CGACCTGCAG CCCGGTGTTC AGTACAACAT CACTATCTAT
    GCTGTGGAGG AGAACCAGGA GAGCACACCC GTTTTCATCC AACAAGAGAC CACTGGCACC
    CCAAGATCTG ATAACGTCCC CCCTCCGACG GACCTACAGT TTGTGGAACT GACTGATGTG
    AAAGTCACCA TCATGTGGAC CCCTCCTGAT AGTGTGGTGT CTGGATACCG TGTGGAGGTC
    CTGCCTGTCA GCCTGCCCGG GGAACATGGG CAGAGGCTGC CTGTCAACAG AAATACCTTT
    GCTGAAATCA CTGGGCTGTC CCCTGGGGTC ACGTACCTCT TCAAAGTCTT TGCTGTGCAC
    CAGGGCAGGG AAAGCAATCC TCTGACGGCA CAACAGACCA CCAAACTCGA CGCTCCCACT
    AACCTCCAGT TTGTCAATGA AACTGACAGA ACAGTTCTGG TAACGTGGAC TCCACCTCGA
    GCCCGTATAG CAGGCTACCG ACTGACCGCG GGCCTGACCC GAGGAGGCCA GCCCAAGCAG
    TACAATGTGG GACCCTTGGC CTCCAAGTAT CCCCTGAGAA ATCTGCAGCC TGGGTCTGAG
    TACACCGTGA CCTTGGTGGC TGTGAAAGGG AACCAGCAGA GTCCCAAAGC CACCGGAGTC
    TTTACTACCC TGCAGCCTCT GCGCTCCATT CCACCTTACA ACACCGAGGT GACAGAGACC
    ACAATTGTGA TCACCTGGAC CCCCGCTCCA AGGATTGGCT TCAAGCTGGG TGTACGACCG
    AGCCAGGGAG GTGAGGCACC CCGAGAAGTG ACTTCAGACT CTGGGAGCAT TGTTGTGTCT
    GGCTTGACTC CAGGCGTGGA ATACACTTAC ACCATCCAAG TCCTGCGAGA TGGCCAGGAG
    AGAGATGCAC CGATTGTCAA CAGAGTAGTG ACACCGCTGT CTCCACCGAC CAACTTGCAT
    CTGGAGGCAA ACCCTGACAC TGGAGTGCTT ACTGTCTCCT GGGAGAGGAG CACTACCCCA
    GATATCACTG GCTACAGAAT AACTACTACC CCCACGAACG GGCAGCAGGG GACCTCTCTG
    GAAGAAGTGG TCCATGCTGA TCAGAGTTCC TGCACTTTTG AGAACCTGAA TCCTGGCCTG
    GAGTACAACG TCAGTGTTTA CACTGTCAAA GATGACAAGG AAAGTGCCCC TATCTCTGAT
    ACCGTTGTCC CAGCTGTCCC TCCTCCCACG GATCTGCGAT TCACCAATAT CGGTCCAGAC
    ACGATGCGGG TCACTTGGGC CCCGCCTCCG TCCATCGAGC TGACCAACCT CTTGGTGCGC
    TACTCACCCG TGAAGAATGA AGAGGACGTT GCAGAGCTAT CCATTTCACC TTCAGACAAT
    GCCGTGGTCC TAACAAATCT CCTGCCTGGG ACAGAATACT TAGTCAGTGT CTCCAGTGTC
    TACGAACAAC ATGAGAGCAT CCCTCTCCGG GGAAGGCAGA AAACAGGTCT CGATTCCCCA
    ACTGGTTTTG ATTCTTCTGA TATCACCGCC AACTCATTCA CTGTCCACTG GGTGGCTCCT
    CGGGCCCCCA TCACCGGCTA CATCATCCGC CATCACGCCG AGCATTCTGT CGGAAGACCC
    AGGCAGGATC GAGTGCCGCC CTCGCGGAAT TCCATCACCC TCACCAACCT TAATCCGGGC
    ACCGAGTACG TTGTCAGCAT CATTGCTGTT AATGGCAGAG AGGAGAGCCC GCCACTGATT
    GGCCAGCAAG CCACAGTTTC TGATATTCCG AGAGATCTGG AGGTCATTGC CTCCACCCCC
    ACCAGCCTGC TCATCAGTTG GGAACCCCCT GCCGTCTCTG TGCGCTATTA CAGAATCACC
    TACGGAGAGA CAGGAGGAAA TAGCCCTGTC CAGGAGTTCA CTGTGCCCGG AAGCAAGTCC
    ACAGCCACCA TCAACAACAT TAAACCAGGA GCAGACTACA CCATCACCCT GTATGCTGTC
    ACTGGCCGTG GGGACAGTCC AGCAAGCAGC AAGCCAGTTT CCATCAATTA TAAAACAGAA
    ATTGACAAGC CGTCCCAGAT GCAGGTGACA GATGTCCAGG ACAACAGCAT CAGTGTCAGG
    TGGCTGCCTT CAACTTCTCC TGTGACAGGC TACAGAGTGA CCACCACTCC CAAAAATGGC
    CTAGGACCAT CAAAAACTAA AACTGCCAGT CCAGATCAAA CAGAAATGAC CATTGAAGGT
    TTGCAACCCA CTGTGGAGTA CGTGGTTAGT GTTTATGCTC AGAACCGGAA CGGAGAAAGC
    CAGCCCCTGG TTCAAACTGC AGTGACCAAC ATTGATCGCC CTAAAGGACT GGCATTCACT
    GATGTGGATG TCGATTCCAT CAAAATTGCT TGGGAAAGCC CACAGGGGCA AGTTTCCAGG
    TACAGGGTGA CCTACTCGAG CCCTGAGGAT GGAATCCGGG AGCTTTTCCC TGCACCTGAT
    GGTGAAGACG ACACTGCAGA GCTGCAGGGC CTCAGGCCGG GGTCTGAGTA CACAGTCAGT
    GTGGTTGCCT TGCACGATGA TATGGAGAGC CAGCCCCTGA TTGGAATCCA GTCCACAGCC
    ATTCCTGCGC CCACCAATCT GAAGTTCAGT CAGGTGACAC CCACCAGCTT TACTGCCCAG
    TGGATAGCAC CCAGTGTTCA GCTCACTGGC TACCGGGTGC GGGTGAACCC GAAAGAGAAG
    ACAGGACCAA TGAAAGAAAT CAACCTTTCT CCAGACAGCT CATCGGTGAT TGTGTCAGGA
    CTCATGGTGG CCACTAAATA CGAAGTCAGT GTCTATGCTC TCAAGGACAC ACTGACAAGC
    AGACCAGCCC AGGGAGTCAT CACTACTCTG GAGAATGTTA GCCCTCCAAG AAGGGCCCGT
    GTGACGGACG CTACAGAGAC CACCATCACT ATTAGCTGGA GAACAAAGAC AGAGACAATC
    ACTGGCTTCC AAGTCGATGC CATCCCAGCC AATGGCCAGA CCCCAGTTCA GAGGAGCATC
    AGCCCGGATG TTAGAAGCTA CACCATTACA GGTTTACAGC CAGGCACTGA CTACAAGATC
    CACCTGTACA CTCTGAACGA CAATGCCCGG AGCTCGCCCG TGATCATCGA TGCCTCCACC
    GCCATCGACG CACCATCCAA CCTGCGGTTC CTGACCACCA CACCCAACTC CTTGCTGGTG
    TCATGGCAGG CGCCCCGTGC CAGGATTACT GGCTACATTA TCAAGTATGA GAAGCCTGGA
    TCCCCTCCCA GAGAAGTGGT CCCTCGGCCC CGCCCTGGTG TCACGGAGGC CACCATTACT
    GGTCTGGAGC CAGGAACCGA GTACACCATC TACGTCATTG CCCTGAAGAA CAATCAGAAG
    AGTGAGCCCC TGATTGGGAG GAAGAAGACA GTTCAAAAGA CCCCCTTCAT CACCAACCCT
    GGGTATGACA CCGAAAATGG TATTCAGCTT CCTGGCACAA CCCACCAGCA ACCCAGTGTT
    GGGCAACAAA TGATCTTTGA GGAACATGGC TTTAGGCGGA CAACGCCACC CACTGCGGCC
    ACCCCCGTCA GGCTTAGGCC AAGACCATAC CTGCCGAATG TAGATGAGGA GGTCCAAATC
    GGTCATGTTC CCAGGGGAGA CGTAGACTAC CACCTCTATC CTCATGTTCC GGGCCTCAAT
    CCAAATGCCT CTACAGGACA GGAAGCTCTC TCTCAGACAA CCATCTCTTG GACGCCGTTC
    CAGGAGAGTT CTGAGTACAT CATTTCATGC CAACCAGTTG GCACCGACGA AGAGCCCTTA
    CAGTTCCAAG TTCCTGGAAC TTCTACCAGT GCGACTCTGA CTGGCCTTAC CAGAGGGGTC
    ACCTACAACA TCATAGTGGA GGCACTGCAG AACCAGAGGA GGCACAAGGT TCGGGAAGAG
    GTTGTGACTG TGGGCAACGC TGTCAGCGAA GGCCTGAACC AGCCTACAGA TGACTCATGC
    TTTGACCCTT ACACGGTTTC CCATTACGCC ATTGGAGAGG AGTGGGAGCG GTTGTCTGAC
    GCTGGCTTTA AGCTCACATG CCAGTGCTTG GGCTTTGGCA GTGGTCATTT CAGATGCGAT
    TCATCTAAAT GGTGCCATGA CAACGGTGTC AACTACAAGA TCGGAGAGAA GTGGGATCGG
    CAGGGAGAAA ATGGCCAGCG GATGAGCTGC ACATGCCTCG GGAATGGAAA GGGAGAATTC
    AAGTGTGATC CCCATGAAGC AACGTGCTAT GACGATGGGA AGACCTACCA TGTAGGAGAA
    CAGTGGCAGA AAGAATATCT CGGAGCCATT TGCTCCTGCA CGTGTTTCGG AGGCCAGCGG
    GGCTGGCGCT GTGACAACTG CCGTAGACCT GGGGCTGCTG AACCCAGTCC CGATGGCACC
    ACCGGCCACA CCTACAACCA GTATACACAG AGATACAATC AGAGAACAAA CACTAACGTA
    AATTGCCCCA TTGAGTGCTT CATGCCGCTA GATGTGCAAG CTGACAGAGA CGATTCTCGA
    GAGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu17547
    Clone ID Related Accession (Same CDS sequence) NM_001276408.1
    Accession Version NM_001276408.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7161bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-24
    Organism Mus musculus(house mouse)
    Product fibronectin isoform b precursor
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from CJ098561.1, CJ099024.1, CN534124.1, AK147683.1, AK147249.1 and AC124821.5. Transcript Variant: This variant (2) lacks an in-frame exon in the coding region, compared to variant 1. The encoded isoform (b) is shorter than isoform a. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK147683.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164134, SAMN01164142 [ECO:0000348] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    ATGCTCAGGG GTCCGGGACC CGGGCGGCTG CTGCTGCTGG CAGTCCTGTG CCTGGGGACC 
    TCGGTGCGCT GCACCGAAGC CGGGAAGAGC AAGAGGCAGG CTCAGCAAAT CGTGCAGCCT
    CAATCCCCGG TGGCTGTCAG TCAGAGCAAG CCTGGCTGTT TTGACAATGG GAAGCACTAT
    CAGATAAATC AGCAGTGGGA ACGGACCTAC CTAGGCAACG CCCTGGTTTG TACCTGCTAT
    GGAGGAAGCC GGGGTTTTAA CTGCGAGAGC AAGCCTGAGC CTGAAGAGAC TTGCTTTGAC
    AAATACACTG GGAACACTTA CAAAGTGGGT GACACTTATG AGCGCCCTAA AGATTCCATG
    ATCTGGGACT GTACCTGCAT CGGGGCTGGG AGAGGCAGGA TCAGCTGTAC CATTGCAAAT
    CGCTGCCATG AAGGGGGTCA GTCCTACAAG ATTGGCGACA AGTGGAGGAG GCCACATGAG
    ACTGGTGGCT ACATGTTAGA GTGTCTGTGT CTGGGAAATG GAAAAGGGGA ATGGACCTGC
    AAACCTATAG CTGAGAAGTG TTTTGATCAT GCTGCTGGGA CGTCCTACGT CGTGGGGGAG
    ACCTGGGAAA AGCCCTACCA AGGCTGGATG ATGGTGGACT GTACTTGTCT AGGCGAAGGC
    AATGGACGCA TCACCTGTAC CTCCAGAAAC AGATGCAACG ATCAGGACAC CCGGACATCC
    TATAGGATTG GAGACACGTG GAGCAAGAAG GACAACCGAG GAAACCTGCT TCAGTGTGTC
    TGCACAGGCA ATGGCAGAGG GGAGTGGAAG TGTGAGCGAC ATGCTCTACA AAGTGCTTCA
    GCCGGATCTG GCTCCTTCAC TGATGTCCGA ACAGCTATTT ACCAACCGCA GACTCACCCC
    CAGCCCGCTC CCTACGGCCA CTGTGTCACC GACAGTGGTG TGGTCTACTC TGTGGGAATG
    CAGTGGCTGA AGTCGCAAGG AAACAAGCAA ATGCTGTGCA CGTGCCTGGG CAATGGCGTC
    AGCTGCCAGG AGACAGCCGT GACCCAGACT TATGGTGGCA ATTCAAACGG GGAGCCCTGT
    GTCCTCCCGT TCACCTACAA CGGTAGGACC TTCTATTCCT GCACCACCGA AGGGCGGCAA
    GACGGACATC TGTGGTGTAG CACAACTTCC AATTACGAAC AAGACCAGAA GTATTCCTTC
    TGCACAGACC ATGCGGTTTT GGTTCAGACT CGAGGCGGAA ATTCCAATGG TGCTCTGTGC
    CACTTCCCCT TCCTGTACAA CAACCGGAAT TACACCGACT GTACTTCTGA GGGTCGCAGG
    GACAACATGA AATGGTGCGG CACCACCCAG AACTACGATG CCGATCAGAA GTTTGGATTC
    TGCCCAATGG CTGCCCACGA GGAGATCTGC ACAACCAATG AAGGGGTCAT GTATCGCATT
    GGGGATCAGT GGGATAAGCA GCATGACCTG GGCCACATGA TGAGGTGCAC GTGTGTGGGG
    AACGGTCGTG GAGAATGGGC CTGCATCCCC TACTCCCAGC TCCGAGACCA GTGCATCGTT
    GATGACATTA CTTACAATGT GAACGACACG TTCCACAAGC GTCACGAGGA GGGACATATG
    CTGAACTGTA CCTGCTTTGG TCAGGGCCGG GGCAGATGGA AGTGTGACCC CATTGACCAG
    TGCCAAGATT CAGAGACCCG GACATTTTAC CAGATTGGTG ACTCCTGGGA GAAGTTTGTG
    CATGGTGTCC GATACCAGTG TTACTGCTAC GGCCGTGGCA TCGGGGAGTG GCACTGTCAA
    CCTCTGCAGA CCTACCCAGG CACAACTGGA CCTGTCCAAG TAATTATCAC GGAGACCCCC
    AGCCAGCCCA ATTCCCACCC CATCCAGTGG AATGCCCCGG AGCCTTCACA CATCACCAAG
    TACATTCTCA GATGGAGACC TAAAACCTCT ACGGGTCGCT GGAAGGAAGC TACCATTCCT
    GGCCACCTTA ACTCCTATAC CATCAAAGGC CTGACCCCAG GTGTGATCTA TGAGGGACAG
    CTCATCAGCA TCCAGCAGTA TGGCCACAGA GAAGTGACAC GCTTCGACTT CACCACCAGC
    GCCAGCACCC CTGTGACCAG CAACACGGTG ACCGGAGAGA CTGCGCCCTA CTCTCCTGTT
    GTGGCCACTT CTGAATCTGT AACTGAAATC ACAGCCAGCA GCTTTGTGGT CTCATGGGTC
    TCAGCCTCCG ACACCGTGTC AGGCTTCCGG GTGGAGTATG AGCTGAGCGA GGAGGGAGAT
    GAACCACAGT ACCTTGATCT CCCAAGCACG GCCACTTCCG TGAACATTCC TGACCTGCTC
    CCGGGCAGAA AGTACATTGT CAATGTCTAT CAGATATCTG AGGAGGGAAA ACAGAGCTTG
    ATCCTGTCTA CCTCACAGAC TACAGCACCT GACGCTCCTC CAGACCCTAC CGTGGACCAG
    GTTGATGATA CTTCCATTGT TGTTCGGTGG AGTAGACCCC AGGCACCTAT CACAGGGTAT
    AGAATTGTCT ATTCACCTTC AGTAGAAGGC AGTAGCACAG AGCTCAACCT CCCTGAAACG
    GCCAACTCCG TCACCCTCAG CGACCTGCAG CCCGGTGTTC AGTACAACAT CACTATCTAT
    GCTGTGGAGG AGAACCAGGA GAGCACACCC GTTTTCATCC AACAAGAGAC CACTGGCACC
    CCAAGATCTG ATAACGTCCC CCCTCCGACG GACCTACAGT TTGTGGAACT GACTGATGTG
    AAAGTCACCA TCATGTGGAC CCCTCCTGAT AGTGTGGTGT CTGGATACCG TGTGGAGGTC
    CTGCCTGTCA GCCTGCCCGG GGAACATGGG CAGAGGCTGC CTGTCAACAG AAATACCTTT
    GCTGAAATCA CTGGGCTGTC CCCTGGGGTC ACGTACCTCT TCAAAGTCTT TGCTGTGCAC
    CAGGGCAGGG AAAGCAATCC TCTGACGGCA CAACAGACCA CCAAACTCGA CGCTCCCACT
    AACCTCCAGT TTGTCAATGA AACTGACAGA ACAGTTCTGG TAACGTGGAC TCCACCTCGA
    GCCCGTATAG CAGGCTACCG ACTGACCGCG GGCCTGACCC GAGGAGGCCA GCCCAAGCAG
    TACAATGTGG GACCCTTGGC CTCCAAGTAT CCCCTGAGAA ATCTGCAGCC TGGGTCTGAG
    TACACCGTGA CCTTGGTGGC TGTGAAAGGG AACCAGCAGA GTCCCAAAGC CACCGGAGTC
    TTTACTACCC TGCAGCCTCT GCGCTCCATT CCACCTTACA ACACCGAGGT GACAGAGACC
    ACAATTGTGA TCACCTGGAC CCCCGCTCCA AGGATTGGCT TCAAGCTGGG TGTACGACCG
    AGCCAGGGAG GTGAGGCACC CCGAGAAGTG ACTTCAGACT CTGGGAGCAT TGTTGTGTCT
    GGCTTGACTC CAGGCGTGGA ATACACTTAC ACCATCCAAG TCCTGCGAGA TGGCCAGGAG
    AGAGATGCAC CGATTGTCAA CAGAGTAGTG ACACCGCTGT CTCCACCGAC CAACTTGCAT
    CTGGAGGCAA ACCCTGACAC TGGAGTGCTT ACTGTCTCCT GGGAGAGGAG CACTACCCCA
    GATATCACTG GCTACAGAAT AACTACTACC CCCACGAACG GGCAGCAGGG GACCTCTCTG
    GAAGAAGTGG TCCATGCTGA TCAGAGTTCC TGCACTTTTG AGAACCTGAA TCCTGGCCTG
    GAGTACAACG TCAGTGTTTA CACTGTCAAA GATGACAAGG AAAGTGCCCC TATCTCTGAT
    ACCGTTGTCC CAGCTGTCCC TCCTCCCACG GATCTGCGAT TCACCAATAT CGGTCCAGAC
    ACGATGCGGG TCACTTGGGC CCCGCCTCCG TCCATCGAGC TGACCAACCT CTTGGTGCGC
    TACTCACCCG TGAAGAATGA AGAGGACGTT GCAGAGCTAT CCATTTCACC TTCAGACAAT
    GCCGTGGTCC TAACAAATCT CCTGCCTGGG ACAGAATACT TAGTCAGTGT CTCCAGTGTC
    TACGAACAAC ATGAGAGCAT CCCTCTCCGG GGAAGGCAGA AAACAGGTCT CGATTCCCCA
    ACTGGTTTTG ATTCTTCTGA TATCACCGCC AACTCATTCA CTGTCCACTG GGTGGCTCCT
    CGGGCCCCCA TCACCGGCTA CATCATCCGC CATCACGCCG AGCATTCTGT CGGAAGACCC
    AGGCAGGATC GAGTGCCGCC CTCGCGGAAT TCCATCACCC TCACCAACCT TAATCCGGGC
    ACCGAGTACG TTGTCAGCAT CATTGCTGTT AATGGCAGAG AGGAGAGCCC GCCACTGATT
    GGCCAGCAAG CCACAGTTTC TGATATTCCG AGAGATCTGG AGGTCATTGC CTCCACCCCC
    ACCAGCCTGC TCATCAGTTG GGAACCCCCT GCCGTCTCTG TGCGCTATTA CAGAATCACC
    TACGGAGAGA CAGGAGGAAA TAGCCCTGTC CAGGAGTTCA CTGTGCCCGG AAGCAAGTCC
    ACAGCCACCA TCAACAACAT TAAACCAGGA GCAGACTACA CCATCACCCT GTATGCTGTC
    ACTGGCCGTG GGGACAGTCC AGCAAGCAGC AAGCCAGTTT CCATCAATTA TAAAACAGAA
    ATTGACAAGC CGTCCCAGAT GCAGGTGACA GATGTCCAGG ACAACAGCAT CAGTGTCAGG
    TGGCTGCCTT CAACTTCTCC TGTGACAGGC TACAGAGTGA CCACCACTCC CAAAAATGGC
    CTAGGACCAT CAAAAACTAA AACTGCCAGT CCAGATCAAA CAGAAATGAC CATTGAAGGT
    TTGCAACCCA CTGTGGAGTA CGTGGTTAGT GTTTATGCTC AGAACCGGAA CGGAGAAAGC
    CAGCCCCTGG TTCAAACTGC AGTGACCAAC ATTGATCGCC CTAAAGGACT GGCATTCACT
    GATGTGGATG TCGATTCCAT CAAAATTGCT TGGGAAAGCC CACAGGGGCA AGTTTCCAGG
    TACAGGGTGA CCTACTCGAG CCCTGAGGAT GGAATCCGGG AGCTTTTCCC TGCACCTGAT
    GGTGAAGACG ACACTGCAGA GCTGCAGGGC CTCAGGCCGG GGTCTGAGTA CACAGTCAGT
    GTGGTTGCCT TGCACGATGA TATGGAGAGC CAGCCCCTGA TTGGAATCCA GTCCACAGCC
    ATTCCTGCGC CCACCAATCT GAAGTTCAGT CAGGTGACAC CCACCAGCTT TACTGCCCAG
    TGGATAGCAC CCAGTGTTCA GCTCACTGGC TACCGGGTGC GGGTGAACCC GAAAGAGAAG
    ACAGGACCAA TGAAAGAAAT CAACCTTTCT CCAGACAGCT CATCGGTGAT TGTGTCAGGA
    CTCATGGTGG CCACTAAATA CGAAGTCAGT GTCTATGCTC TCAAGGACAC ACTGACAAGC
    AGACCAGCCC AGGGAGTCAT CACTACTCTG GAGAATGTTA GCCCTCCAAG AAGGGCCCGT
    GTGACGGACG CTACAGAGAC CACCATCACT ATTAGCTGGA GAACAAAGAC AGAGACAATC
    ACTGGCTTCC AAGTCGATGC CATCCCAGCC AATGGCCAGA CCCCAGTTCA GAGGAGCATC
    AGCCCGGATG TTAGAAGCTA CACCATTACA GGTTTACAGC CAGGCACTGA CTACAAGATC
    CACCTGTACA CTCTGAACGA CAATGCCCGG AGCTCGCCCG TGATCATCGA TGCCTCCACC
    GCCATCGACG CACCATCCAA CCTGCGGTTC CTGACCACCA CACCCAACTC CTTGCTGGTG
    TCATGGCAGG CGCCCCGTGC CAGGATTACT GGCTACATTA TCAAGTATGA GAAGCCTGGA
    TCCCCTCCCA GAGAAGTGGT CCCTCGGCCC CGCCCTGGTG TCACGGAGGC CACCATTACT
    GGTCTGGAGC CAGGAACCGA GTACACCATC TACGTCATTG CCCTGAAGAA CAATCAGAAG
    AGTGAGCCCC TGATTGGGAG GAAGAAGACA GATGAGCTTC CCCAACTGGT TACCCTTCCA
    CACCCCAATC TTCATGGACC AGAGATCTTG GATGTTCCCT CCACAGTTCA AAAGACCCCC
    TTCATCACCA ACCCTGGGTA TGACACCGAA AATGGTATTC AGCTTCCTGG CACAACCCAC
    CAGCAACCCA GTGTTGGGCA ACAAATGATC TTTGAGGAAC ATGGCTTTAG GCGGACAACG
    CCACCCACTG CGGCCACCCC CGTCAGGCTT AGGCCAAGAC CATACCTGCC GAATGTAGAT
    GAGGAGGTCC AAATCGGTCA TGTTCCCAGG GGAGACGTAG ACTACCACCT CTATCCTCAT
    GTTCCGGGCC TCAATCCAAA TGCCTCTACA GGACAGGAAG CTCTCTCTCA GACAACCATC
    TCTTGGACGC CGTTCCAGGA GAGTTCTGAG TACATCATTT CATGCCAACC AGTTGGCACC
    GACGAAGAGC CCTTACAGTT CCAAGTTCCT GGAACTTCTA CCAGTGCGAC TCTGACTGGC
    CTTACCAGAG GGGTCACCTA CAACATCATA GTGGAGGCAC TGCAGAACCA GAGGAGGCAC
    AAGGTTCGGG AAGAGGTTGT GACTGTGGGC AACGCTGTCA GCGAAGGCCT GAACCAGCCT
    ACAGATGACT CATGCTTTGA CCCTTACACG GTTTCCCATT ACGCCATTGG AGAGGAGTGG
    GAGCGGTTGT CTGACGCTGG CTTTAAGCTC ACATGCCAGT GCTTGGGCTT TGGCAGTGGT
    CATTTCAGAT GCGATTCATC TAAATGGTGC CATGACAACG GTGTCAACTA CAAGATCGGA
    GAGAAGTGGG ATCGGCAGGG AGAAAATGGC CAGCGGATGA GCTGCACATG CCTCGGGAAT
    GGAAAGGGAG AATTCAAGTG TGATCCCCAT GAAGCAACGT GCTATGACGA TGGGAAGACC
    TACCATGTAG GAGAACAGTG GCAGAAAGAA TATCTCGGAG CCATTTGCTC CTGCACGTGT
    TTCGGAGGCC AGCGGGGCTG GCGCTGTGAC AACTGCCGTA GACCTGGGGC TGCTGAACCC
    AGTCCCGATG GCACCACCGG CCACACCTAC AACCAGTATA CACAGAGATA CAATCAGAGA
    ACAAACACTA ACGTAAATTG CCCCATTGAG TGCTTCATGC CGCTAGATGT GCAAGCTGAC
    AGAGACGATT CTCGAGAGTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001263337.1
    CDS253..7413
    Translation

    Target ORF information:

    RefSeq Version NM_001276408.1
    Organism Mus musculus(house mouse)
    Definition Mus musculus fibronectin 1 (Fn1), transcript variant 2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001276408.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    ATGCTCAGGG GTCCGGGACC CGGGCGGCTG CTGCTGCTGG CAGTCCTGTG CCTGGGGACC 
    TCGGTGCGCT GCACCGAAGC CGGGAAGAGC AAGAGGCAGG CTCAGCAAAT CGTGCAGCCT
    CAATCCCCGG TGGCTGTCAG TCAGAGCAAG CCTGGCTGTT TTGACAATGG GAAGCACTAT
    CAGATAAATC AGCAGTGGGA ACGGACCTAC CTAGGCAACG CCCTGGTTTG TACCTGCTAT
    GGAGGAAGCC GGGGTTTTAA CTGCGAGAGC AAGCCTGAGC CTGAAGAGAC TTGCTTTGAC
    AAATACACTG GGAACACTTA CAAAGTGGGT GACACTTATG AGCGCCCTAA AGATTCCATG
    ATCTGGGACT GTACCTGCAT CGGGGCTGGG AGAGGCAGGA TCAGCTGTAC CATTGCAAAT
    CGCTGCCATG AAGGGGGTCA GTCCTACAAG ATTGGCGACA AGTGGAGGAG GCCACATGAG
    ACTGGTGGCT ACATGTTAGA GTGTCTGTGT CTGGGAAATG GAAAAGGGGA ATGGACCTGC
    AAACCTATAG CTGAGAAGTG TTTTGATCAT GCTGCTGGGA CGTCCTACGT CGTGGGGGAG
    ACCTGGGAAA AGCCCTACCA AGGCTGGATG ATGGTGGACT GTACTTGTCT AGGCGAAGGC
    AATGGACGCA TCACCTGTAC CTCCAGAAAC AGATGCAACG ATCAGGACAC CCGGACATCC
    TATAGGATTG GAGACACGTG GAGCAAGAAG GACAACCGAG GAAACCTGCT TCAGTGTGTC
    TGCACAGGCA ATGGCAGAGG GGAGTGGAAG TGTGAGCGAC ATGCTCTACA AAGTGCTTCA
    GCCGGATCTG GCTCCTTCAC TGATGTCCGA ACAGCTATTT ACCAACCGCA GACTCACCCC
    CAGCCCGCTC CCTACGGCCA CTGTGTCACC GACAGTGGTG TGGTCTACTC TGTGGGAATG
    CAGTGGCTGA AGTCGCAAGG AAACAAGCAA ATGCTGTGCA CGTGCCTGGG CAATGGCGTC
    AGCTGCCAGG AGACAGCCGT GACCCAGACT TATGGTGGCA ATTCAAACGG GGAGCCCTGT
    GTCCTCCCGT TCACCTACAA CGGTAGGACC TTCTATTCCT GCACCACCGA AGGGCGGCAA
    GACGGACATC TGTGGTGTAG CACAACTTCC AATTACGAAC AAGACCAGAA GTATTCCTTC
    TGCACAGACC ATGCGGTTTT GGTTCAGACT CGAGGCGGAA ATTCCAATGG TGCTCTGTGC
    CACTTCCCCT TCCTGTACAA CAACCGGAAT TACACCGACT GTACTTCTGA GGGTCGCAGG
    GACAACATGA AATGGTGCGG CACCACCCAG AACTACGATG CCGATCAGAA GTTTGGATTC
    TGCCCAATGG CTGCCCACGA GGAGATCTGC ACAACCAATG AAGGGGTCAT GTATCGCATT
    GGGGATCAGT GGGATAAGCA GCATGACCTG GGCCACATGA TGAGGTGCAC GTGTGTGGGG
    AACGGTCGTG GAGAATGGGC CTGCATCCCC TACTCCCAGC TCCGAGACCA GTGCATCGTT
    GATGACATTA CTTACAATGT GAACGACACG TTCCACAAGC GTCACGAGGA GGGACATATG
    CTGAACTGTA CCTGCTTTGG TCAGGGCCGG GGCAGATGGA AGTGTGACCC CATTGACCAG
    TGCCAAGATT CAGAGACCCG GACATTTTAC CAGATTGGTG ACTCCTGGGA GAAGTTTGTG
    CATGGTGTCC GATACCAGTG TTACTGCTAC GGCCGTGGCA TCGGGGAGTG GCACTGTCAA
    CCTCTGCAGA CCTACCCAGG CACAACTGGA CCTGTCCAAG TAATTATCAC GGAGACCCCC
    AGCCAGCCCA ATTCCCACCC CATCCAGTGG AATGCCCCGG AGCCTTCACA CATCACCAAG
    TACATTCTCA GATGGAGACC TAAAACCTCT ACGGGTCGCT GGAAGGAAGC TACCATTCCT
    GGCCACCTTA ACTCCTATAC CATCAAAGGC CTGACCCCAG GTGTGATCTA TGAGGGACAG
    CTCATCAGCA TCCAGCAGTA TGGCCACAGA GAAGTGACAC GCTTCGACTT CACCACCAGC
    GCCAGCACCC CTGTGACCAG CAACACGGTG ACCGGAGAGA CTGCGCCCTA CTCTCCTGTT
    GTGGCCACTT CTGAATCTGT AACTGAAATC ACAGCCAGCA GCTTTGTGGT CTCATGGGTC
    TCAGCCTCCG ACACCGTGTC AGGCTTCCGG GTGGAGTATG AGCTGAGCGA GGAGGGAGAT
    GAACCACAGT ACCTTGATCT CCCAAGCACG GCCACTTCCG TGAACATTCC TGACCTGCTC
    CCGGGCAGAA AGTACATTGT CAATGTCTAT CAGATATCTG AGGAGGGAAA ACAGAGCTTG
    ATCCTGTCTA CCTCACAGAC TACAGCACCT GACGCTCCTC CAGACCCTAC CGTGGACCAG
    GTTGATGATA CTTCCATTGT TGTTCGGTGG AGTAGACCCC AGGCACCTAT CACAGGGTAT
    AGAATTGTCT ATTCACCTTC AGTAGAAGGC AGTAGCACAG AGCTCAACCT CCCTGAAACG
    GCCAACTCCG TCACCCTCAG CGACCTGCAG CCCGGTGTTC AGTACAACAT CACTATCTAT
    GCTGTGGAGG AGAACCAGGA GAGCACACCC GTTTTCATCC AACAAGAGAC CACTGGCACC
    CCAAGATCTG ATAACGTCCC CCCTCCGACG GACCTACAGT TTGTGGAACT GACTGATGTG
    AAAGTCACCA TCATGTGGAC CCCTCCTGAT AGTGTGGTGT CTGGATACCG TGTGGAGGTC
    CTGCCTGTCA GCCTGCCCGG GGAACATGGG CAGAGGCTGC CTGTCAACAG AAATACCTTT
    GCTGAAATCA CTGGGCTGTC CCCTGGGGTC ACGTACCTCT TCAAAGTCTT TGCTGTGCAC
    CAGGGCAGGG AAAGCAATCC TCTGACGGCA CAACAGACCA CCAAACTCGA CGCTCCCACT
    AACCTCCAGT TTGTCAATGA AACTGACAGA ACAGTTCTGG TAACGTGGAC TCCACCTCGA
    GCCCGTATAG CAGGCTACCG ACTGACCGCG GGCCTGACCC GAGGAGGCCA GCCCAAGCAG
    TACAATGTGG GACCCTTGGC CTCCAAGTAT CCCCTGAGAA ATCTGCAGCC TGGGTCTGAG
    TACACCGTGA CCTTGGTGGC TGTGAAAGGG AACCAGCAGA GTCCCAAAGC CACCGGAGTC
    TTTACTACCC TGCAGCCTCT GCGCTCCATT CCACCTTACA ACACCGAGGT GACAGAGACC
    ACAATTGTGA TCACCTGGAC CCCCGCTCCA AGGATTGGCT TCAAGCTGGG TGTACGACCG
    AGCCAGGGAG GTGAGGCACC CCGAGAAGTG ACTTCAGACT CTGGGAGCAT TGTTGTGTCT
    GGCTTGACTC CAGGCGTGGA ATACACTTAC ACCATCCAAG TCCTGCGAGA TGGCCAGGAG
    AGAGATGCAC CGATTGTCAA CAGAGTAGTG ACACCGCTGT CTCCACCGAC CAACTTGCAT
    CTGGAGGCAA ACCCTGACAC TGGAGTGCTT ACTGTCTCCT GGGAGAGGAG CACTACCCCA
    GATATCACTG GCTACAGAAT AACTACTACC CCCACGAACG GGCAGCAGGG GACCTCTCTG
    GAAGAAGTGG TCCATGCTGA TCAGAGTTCC TGCACTTTTG AGAACCTGAA TCCTGGCCTG
    GAGTACAACG TCAGTGTTTA CACTGTCAAA GATGACAAGG AAAGTGCCCC TATCTCTGAT
    ACCGTTGTCC CAGCTGTCCC TCCTCCCACG GATCTGCGAT TCACCAATAT CGGTCCAGAC
    ACGATGCGGG TCACTTGGGC CCCGCCTCCG TCCATCGAGC TGACCAACCT CTTGGTGCGC
    TACTCACCCG TGAAGAATGA AGAGGACGTT GCAGAGCTAT CCATTTCACC TTCAGACAAT
    GCCGTGGTCC TAACAAATCT CCTGCCTGGG ACAGAATACT TAGTCAGTGT CTCCAGTGTC
    TACGAACAAC ATGAGAGCAT CCCTCTCCGG GGAAGGCAGA AAACAGGTCT CGATTCCCCA
    ACTGGTTTTG ATTCTTCTGA TATCACCGCC AACTCATTCA CTGTCCACTG GGTGGCTCCT
    CGGGCCCCCA TCACCGGCTA CATCATCCGC CATCACGCCG AGCATTCTGT CGGAAGACCC
    AGGCAGGATC GAGTGCCGCC CTCGCGGAAT TCCATCACCC TCACCAACCT TAATCCGGGC
    ACCGAGTACG TTGTCAGCAT CATTGCTGTT AATGGCAGAG AGGAGAGCCC GCCACTGATT
    GGCCAGCAAG CCACAGTTTC TGATATTCCG AGAGATCTGG AGGTCATTGC CTCCACCCCC
    ACCAGCCTGC TCATCAGTTG GGAACCCCCT GCCGTCTCTG TGCGCTATTA CAGAATCACC
    TACGGAGAGA CAGGAGGAAA TAGCCCTGTC CAGGAGTTCA CTGTGCCCGG AAGCAAGTCC
    ACAGCCACCA TCAACAACAT TAAACCAGGA GCAGACTACA CCATCACCCT GTATGCTGTC
    ACTGGCCGTG GGGACAGTCC AGCAAGCAGC AAGCCAGTTT CCATCAATTA TAAAACAGAA
    ATTGACAAGC CGTCCCAGAT GCAGGTGACA GATGTCCAGG ACAACAGCAT CAGTGTCAGG
    TGGCTGCCTT CAACTTCTCC TGTGACAGGC TACAGAGTGA CCACCACTCC CAAAAATGGC
    CTAGGACCAT CAAAAACTAA AACTGCCAGT CCAGATCAAA CAGAAATGAC CATTGAAGGT
    TTGCAACCCA CTGTGGAGTA CGTGGTTAGT GTTTATGCTC AGAACCGGAA CGGAGAAAGC
    CAGCCCCTGG TTCAAACTGC AGTGACCAAC ATTGATCGCC CTAAAGGACT GGCATTCACT
    GATGTGGATG TCGATTCCAT CAAAATTGCT TGGGAAAGCC CACAGGGGCA AGTTTCCAGG
    TACAGGGTGA CCTACTCGAG CCCTGAGGAT GGAATCCGGG AGCTTTTCCC TGCACCTGAT
    GGTGAAGACG ACACTGCAGA GCTGCAGGGC CTCAGGCCGG GGTCTGAGTA CACAGTCAGT
    GTGGTTGCCT TGCACGATGA TATGGAGAGC CAGCCCCTGA TTGGAATCCA GTCCACAGCC
    ATTCCTGCGC CCACCAATCT GAAGTTCAGT CAGGTGACAC CCACCAGCTT TACTGCCCAG
    TGGATAGCAC CCAGTGTTCA GCTCACTGGC TACCGGGTGC GGGTGAACCC GAAAGAGAAG
    ACAGGACCAA TGAAAGAAAT CAACCTTTCT CCAGACAGCT CATCGGTGAT TGTGTCAGGA
    CTCATGGTGG CCACTAAATA CGAAGTCAGT GTCTATGCTC TCAAGGACAC ACTGACAAGC
    AGACCAGCCC AGGGAGTCAT CACTACTCTG GAGAATGTTA GCCCTCCAAG AAGGGCCCGT
    GTGACGGACG CTACAGAGAC CACCATCACT ATTAGCTGGA GAACAAAGAC AGAGACAATC
    ACTGGCTTCC AAGTCGATGC CATCCCAGCC AATGGCCAGA CCCCAGTTCA GAGGAGCATC
    AGCCCGGATG TTAGAAGCTA CACCATTACA GGTTTACAGC CAGGCACTGA CTACAAGATC
    CACCTGTACA CTCTGAACGA CAATGCCCGG AGCTCGCCCG TGATCATCGA TGCCTCCACC
    GCCATCGACG CACCATCCAA CCTGCGGTTC CTGACCACCA CACCCAACTC CTTGCTGGTG
    TCATGGCAGG CGCCCCGTGC CAGGATTACT GGCTACATTA TCAAGTATGA GAAGCCTGGA
    TCCCCTCCCA GAGAAGTGGT CCCTCGGCCC CGCCCTGGTG TCACGGAGGC CACCATTACT
    GGTCTGGAGC CAGGAACCGA GTACACCATC TACGTCATTG CCCTGAAGAA CAATCAGAAG
    AGTGAGCCCC TGATTGGGAG GAAGAAGACA GATGAGCTTC CCCAACTGGT TACCCTTCCA
    CACCCCAATC TTCATGGACC AGAGATCTTG GATGTTCCCT CCACAGTTCA AAAGACCCCC
    TTCATCACCA ACCCTGGGTA TGACACCGAA AATGGTATTC AGCTTCCTGG CACAACCCAC
    CAGCAACCCA GTGTTGGGCA ACAAATGATC TTTGAGGAAC ATGGCTTTAG GCGGACAACG
    CCACCCACTG CGGCCACCCC CGTCAGGCTT AGGCCAAGAC CATACCTGCC GAATGTAGAT
    GAGGAGGTCC AAATCGGTCA TGTTCCCAGG GGAGACGTAG ACTACCACCT CTATCCTCAT
    GTTCCGGGCC TCAATCCAAA TGCCTCTACA GGACAGGAAG CTCTCTCTCA GACAACCATC
    TCTTGGACGC CGTTCCAGGA GAGTTCTGAG TACATCATTT CATGCCAACC AGTTGGCACC
    GACGAAGAGC CCTTACAGTT CCAAGTTCCT GGAACTTCTA CCAGTGCGAC TCTGACTGGC
    CTTACCAGAG GGGTCACCTA CAACATCATA GTGGAGGCAC TGCAGAACCA GAGGAGGCAC
    AAGGTTCGGG AAGAGGTTGT GACTGTGGGC AACGCTGTCA GCGAAGGCCT GAACCAGCCT
    ACAGATGACT CATGCTTTGA CCCTTACACG GTTTCCCATT ACGCCATTGG AGAGGAGTGG
    GAGCGGTTGT CTGACGCTGG CTTTAAGCTC ACATGCCAGT GCTTGGGCTT TGGCAGTGGT
    CATTTCAGAT GCGATTCATC TAAATGGTGC CATGACAACG GTGTCAACTA CAAGATCGGA
    GAGAAGTGGG ATCGGCAGGG AGAAAATGGC CAGCGGATGA GCTGCACATG CCTCGGGAAT
    GGAAAGGGAG AATTCAAGTG TGATCCCCAT GAAGCAACGT GCTATGACGA TGGGAAGACC
    TACCATGTAG GAGAACAGTG GCAGAAAGAA TATCTCGGAG CCATTTGCTC CTGCACGTGT
    TTCGGAGGCC AGCGGGGCTG GCGCTGTGAC AACTGCCGTA GACCTGGGGC TGCTGAACCC
    AGTCCCGATG GCACCACCGG CCACACCTAC AACCAGTATA CACAGAGATA CAATCAGAGA
    ACAAACACTA ACGTAAATTG CCCCATTGAG TGCTTCATGC CGCTAGATGT GCAAGCTGAC
    AGAGACGATT CTCGAGAGTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu17657
    Clone ID Related Accession (Same CDS sequence) NM_010233.2
    Accession Version NM_010233.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7434bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-24
    Organism Mus musculus(house mouse)
    Product fibronectin isoform a precursor
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from CJ098561.1, CN534124.1, CF166960.1, CK638401.1, CK637170.1, CN538894.1, CN694596.1, CF727068.1, CN460535.1, AK133868.1 and AC124821.5. On Feb 9, 2013 this sequence version replaced NM_010233.1. Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (a). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK090135.1, AK090130.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164134, SAMN01164142 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    ATGCTCAGGG GTCCGGGACC CGGGCGGCTG CTGCTGCTGG CAGTCCTGTG CCTGGGGACC 
    TCGGTGCGCT GCACCGAAGC CGGGAAGAGC AAGAGGCAGG CTCAGCAAAT CGTGCAGCCT
    CAATCCCCGG TGGCTGTCAG TCAGAGCAAG CCTGGCTGTT TTGACAATGG GAAGCACTAT
    CAGATAAATC AGCAGTGGGA ACGGACCTAC CTAGGCAACG CCCTGGTTTG TACCTGCTAT
    GGAGGAAGCC GGGGTTTTAA CTGCGAGAGC AAGCCTGAGC CTGAAGAGAC TTGCTTTGAC
    AAATACACTG GGAACACTTA CAAAGTGGGT GACACTTATG AGCGCCCTAA AGATTCCATG
    ATCTGGGACT GTACCTGCAT CGGGGCTGGG AGAGGCAGGA TCAGCTGTAC CATTGCAAAT
    CGCTGCCATG AAGGGGGTCA GTCCTACAAG ATTGGCGACA AGTGGAGGAG GCCACATGAG
    ACTGGTGGCT ACATGTTAGA GTGTCTGTGT CTGGGAAATG GAAAAGGGGA ATGGACCTGC
    AAACCTATAG CTGAGAAGTG TTTTGATCAT GCTGCTGGGA CGTCCTACGT CGTGGGGGAG
    ACCTGGGAAA AGCCCTACCA AGGCTGGATG ATGGTGGACT GTACTTGTCT AGGCGAAGGC
    AATGGACGCA TCACCTGTAC CTCCAGAAAC AGATGCAACG ATCAGGACAC CCGGACATCC
    TATAGGATTG GAGACACGTG GAGCAAGAAG GACAACCGAG GAAACCTGCT TCAGTGTGTC
    TGCACAGGCA ATGGCAGAGG GGAGTGGAAG TGTGAGCGAC ATGCTCTACA AAGTGCTTCA
    GCCGGATCTG GCTCCTTCAC TGATGTCCGA ACAGCTATTT ACCAACCGCA GACTCACCCC
    CAGCCCGCTC CCTACGGCCA CTGTGTCACC GACAGTGGTG TGGTCTACTC TGTGGGAATG
    CAGTGGCTGA AGTCGCAAGG AAACAAGCAA ATGCTGTGCA CGTGCCTGGG CAATGGCGTC
    AGCTGCCAGG AGACAGCCGT GACCCAGACT TATGGTGGCA ATTCAAACGG GGAGCCCTGT
    GTCCTCCCGT TCACCTACAA CGGTAGGACC TTCTATTCCT GCACCACCGA AGGGCGGCAA
    GACGGACATC TGTGGTGTAG CACAACTTCC AATTACGAAC AAGACCAGAA GTATTCCTTC
    TGCACAGACC ATGCGGTTTT GGTTCAGACT CGAGGCGGAA ATTCCAATGG TGCTCTGTGC
    CACTTCCCCT TCCTGTACAA CAACCGGAAT TACACCGACT GTACTTCTGA GGGTCGCAGG
    GACAACATGA AATGGTGCGG CACCACCCAG AACTACGATG CCGATCAGAA GTTTGGATTC
    TGCCCAATGG CTGCCCACGA GGAGATCTGC ACAACCAATG AAGGGGTCAT GTATCGCATT
    GGGGATCAGT GGGATAAGCA GCATGACCTG GGCCACATGA TGAGGTGCAC GTGTGTGGGG
    AACGGTCGTG GAGAATGGGC CTGCATCCCC TACTCCCAGC TCCGAGACCA GTGCATCGTT
    GATGACATTA CTTACAATGT GAACGACACG TTCCACAAGC GTCACGAGGA GGGACATATG
    CTGAACTGTA CCTGCTTTGG TCAGGGCCGG GGCAGATGGA AGTGTGACCC CATTGACCAG
    TGCCAAGATT CAGAGACCCG GACATTTTAC CAGATTGGTG ACTCCTGGGA GAAGTTTGTG
    CATGGTGTCC GATACCAGTG TTACTGCTAC GGCCGTGGCA TCGGGGAGTG GCACTGTCAA
    CCTCTGCAGA CCTACCCAGG CACAACTGGA CCTGTCCAAG TAATTATCAC GGAGACCCCC
    AGCCAGCCCA ATTCCCACCC CATCCAGTGG AATGCCCCGG AGCCTTCACA CATCACCAAG
    TACATTCTCA GATGGAGACC TAAAACCTCT ACGGGTCGCT GGAAGGAAGC TACCATTCCT
    GGCCACCTTA ACTCCTATAC CATCAAAGGC CTGACCCCAG GTGTGATCTA TGAGGGACAG
    CTCATCAGCA TCCAGCAGTA TGGCCACAGA GAAGTGACAC GCTTCGACTT CACCACCAGC
    GCCAGCACCC CTGTGACCAG CAACACGGTG ACCGGAGAGA CTGCGCCCTA CTCTCCTGTT
    GTGGCCACTT CTGAATCTGT AACTGAAATC ACAGCCAGCA GCTTTGTGGT CTCATGGGTC
    TCAGCCTCCG ACACCGTGTC AGGCTTCCGG GTGGAGTATG AGCTGAGCGA GGAGGGAGAT
    GAACCACAGT ACCTTGATCT CCCAAGCACG GCCACTTCCG TGAACATTCC TGACCTGCTC
    CCGGGCAGAA AGTACATTGT CAATGTCTAT CAGATATCTG AGGAGGGAAA ACAGAGCTTG
    ATCCTGTCTA CCTCACAGAC TACAGCACCT GACGCTCCTC CAGACCCTAC CGTGGACCAG
    GTTGATGATA CTTCCATTGT TGTTCGGTGG AGTAGACCCC AGGCACCTAT CACAGGGTAT
    AGAATTGTCT ATTCACCTTC AGTAGAAGGC AGTAGCACAG AGCTCAACCT CCCTGAAACG
    GCCAACTCCG TCACCCTCAG CGACCTGCAG CCCGGTGTTC AGTACAACAT CACTATCTAT
    GCTGTGGAGG AGAACCAGGA GAGCACACCC GTTTTCATCC AACAAGAGAC CACTGGCACC
    CCAAGATCTG ATAACGTCCC CCCTCCGACG GACCTACAGT TTGTGGAACT GACTGATGTG
    AAAGTCACCA TCATGTGGAC CCCTCCTGAT AGTGTGGTGT CTGGATACCG TGTGGAGGTC
    CTGCCTGTCA GCCTGCCCGG GGAACATGGG CAGAGGCTGC CTGTCAACAG AAATACCTTT
    GCTGAAATCA CTGGGCTGTC CCCTGGGGTC ACGTACCTCT TCAAAGTCTT TGCTGTGCAC
    CAGGGCAGGG AAAGCAATCC TCTGACGGCA CAACAGACCA CCAAACTCGA CGCTCCCACT
    AACCTCCAGT TTGTCAATGA AACTGACAGA ACAGTTCTGG TAACGTGGAC TCCACCTCGA
    GCCCGTATAG CAGGCTACCG ACTGACCGCG GGCCTGACCC GAGGAGGCCA GCCCAAGCAG
    TACAATGTGG GACCCTTGGC CTCCAAGTAT CCCCTGAGAA ATCTGCAGCC TGGGTCTGAG
    TACACCGTGA CCTTGGTGGC TGTGAAAGGG AACCAGCAGA GTCCCAAAGC CACCGGAGTC
    TTTACTACCC TGCAGCCTCT GCGCTCCATT CCACCTTACA ACACCGAGGT GACAGAGACC
    ACAATTGTGA TCACCTGGAC CCCCGCTCCA AGGATTGGCT TCAAGCTGGG TGTACGACCG
    AGCCAGGGAG GTGAGGCACC CCGAGAAGTG ACTTCAGACT CTGGGAGCAT TGTTGTGTCT
    GGCTTGACTC CAGGCGTGGA ATACACTTAC ACCATCCAAG TCCTGCGAGA TGGCCAGGAG
    AGAGATGCAC CGATTGTCAA CAGAGTAGTG ACACCGCTGT CTCCACCGAC CAACTTGCAT
    CTGGAGGCAA ACCCTGACAC TGGAGTGCTT ACTGTCTCCT GGGAGAGGAG CACTACCCCA
    GATATCACTG GCTACAGAAT AACTACTACC CCCACGAACG GGCAGCAGGG GACCTCTCTG
    GAAGAAGTGG TCCATGCTGA TCAGAGTTCC TGCACTTTTG AGAACCTGAA TCCTGGCCTG
    GAGTACAACG TCAGTGTTTA CACTGTCAAA GATGACAAGG AAAGTGCCCC TATCTCTGAT
    ACCGTTGTCC CAGAGGTGCC CCAGCTCACT GACCTAAGCT TTGTTGATAT AACTGATTCA
    AGCATCGGCC TGAGGTGGAC CCCGCTAAAC TCTTCCACCA TTATCGGGTA CCGAATCACA
    GTAGTTGCGG CAGGAGAAGG GATCCCTATT TTTGAAGATT TTGTGGACTC CTCAGTAGGA
    TACTACACAG TTACAGGGCT GGAGCCTGGC ATTGACTATG ACATCAGCGT TATCACTCTC
    ATTAATGGCG GAGAGAGTGC CCCTACTACA CTGACACAGC AAACGGCTGT CCCTCCTCCC
    ACGGATCTGC GATTCACCAA TATCGGTCCA GACACGATGC GGGTCACTTG GGCCCCGCCT
    CCGTCCATCG AGCTGACCAA CCTCTTGGTG CGCTACTCAC CCGTGAAGAA TGAAGAGGAC
    GTTGCAGAGC TATCCATTTC ACCTTCAGAC AATGCCGTGG TCCTAACAAA TCTCCTGCCT
    GGGACAGAAT ACTTAGTCAG TGTCTCCAGT GTCTACGAAC AACATGAGAG CATCCCTCTC
    CGGGGAAGGC AGAAAACAGG TCTCGATTCC CCAACTGGTT TTGATTCTTC TGATATCACC
    GCCAACTCAT TCACTGTCCA CTGGGTGGCT CCTCGGGCCC CCATCACCGG CTACATCATC
    CGCCATCACG CCGAGCATTC TGTCGGAAGA CCCAGGCAGG ATCGAGTGCC GCCCTCGCGG
    AATTCCATCA CCCTCACCAA CCTTAATCCG GGCACCGAGT ACGTTGTCAG CATCATTGCT
    GTTAATGGCA GAGAGGAGAG CCCGCCACTG ATTGGCCAGC AAGCCACAGT TTCTGATATT
    CCGAGAGATC TGGAGGTCAT TGCCTCCACC CCCACCAGCC TGCTCATCAG TTGGGAACCC
    CCTGCCGTCT CTGTGCGCTA TTACAGAATC ACCTACGGAG AGACAGGAGG AAATAGCCCT
    GTCCAGGAGT TCACTGTGCC CGGAAGCAAG TCCACAGCCA CCATCAACAA CATTAAACCA
    GGAGCAGACT ACACCATCAC CCTGTATGCT GTCACTGGCC GTGGGGACAG TCCAGCAAGC
    AGCAAGCCAG TTTCCATCAA TTATAAAACA GAAATTGACA AGCCGTCCCA GATGCAGGTG
    ACAGATGTCC AGGACAACAG CATCAGTGTC AGGTGGCTGC CTTCAACTTC TCCTGTGACA
    GGCTACAGAG TGACCACCAC TCCCAAAAAT GGCCTAGGAC CATCAAAAAC TAAAACTGCC
    AGTCCAGATC AAACAGAAAT GACCATTGAA GGTTTGCAAC CCACTGTGGA GTACGTGGTT
    AGTGTTTATG CTCAGAACCG GAACGGAGAA AGCCAGCCCC TGGTTCAAAC TGCAGTGACC
    AACATTGATC GCCCTAAAGG ACTGGCATTC ACTGATGTGG ATGTCGATTC CATCAAAATT
    GCTTGGGAAA GCCCACAGGG GCAAGTTTCC AGGTACAGGG TGACCTACTC GAGCCCTGAG
    GATGGAATCC GGGAGCTTTT CCCTGCACCT GATGGTGAAG ACGACACTGC AGAGCTGCAG
    GGCCTCAGGC CGGGGTCTGA GTACACAGTC AGTGTGGTTG CCTTGCACGA TGATATGGAG
    AGCCAGCCCC TGATTGGAAT CCAGTCCACA GCCATTCCTG CGCCCACCAA TCTGAAGTTC
    AGTCAGGTGA CACCCACCAG CTTTACTGCC CAGTGGATAG CACCCAGTGT TCAGCTCACT
    GGCTACCGGG TGCGGGTGAA CCCGAAAGAG AAGACAGGAC CAATGAAAGA AATCAACCTT
    TCTCCAGACA GCTCATCGGT GATTGTGTCA GGACTCATGG TGGCCACTAA ATACGAAGTC
    AGTGTCTATG CTCTCAAGGA CACACTGACA AGCAGACCAG CCCAGGGAGT CATCACTACT
    CTGGAGAATG TTAGCCCTCC AAGAAGGGCC CGTGTGACGG ACGCTACAGA GACCACCATC
    ACTATTAGCT GGAGAACAAA GACAGAGACA ATCACTGGCT TCCAAGTCGA TGCCATCCCA
    GCCAATGGCC AGACCCCAGT TCAGAGGAGC ATCAGCCCGG ATGTTAGAAG CTACACCATT
    ACAGGTTTAC AGCCAGGCAC TGACTACAAG ATCCACCTGT ACACTCTGAA CGACAATGCC
    CGGAGCTCGC CCGTGATCAT CGATGCCTCC ACCGCCATCG ACGCACCATC CAACCTGCGG
    TTCCTGACCA CCACACCCAA CTCCTTGCTG GTGTCATGGC AGGCGCCCCG TGCCAGGATT
    ACTGGCTACA TTATCAAGTA TGAGAAGCCT GGATCCCCTC CCAGAGAAGT GGTCCCTCGG
    CCCCGCCCTG GTGTCACGGA GGCCACCATT ACTGGTCTGG AGCCAGGAAC CGAGTACACC
    ATCTACGTCA TTGCCCTGAA GAACAATCAG AAGAGTGAGC CCCTGATTGG GAGGAAGAAG
    ACAGATGAGC TTCCCCAACT GGTTACCCTT CCACACCCCA ATCTTCATGG ACCAGAGATC
    TTGGATGTTC CCTCCACAGT TCAAAAGACC CCCTTCATCA CCAACCCTGG GTATGACACC
    GAAAATGGTA TTCAGCTTCC TGGCACAACC CACCAGCAAC CCAGTGTTGG GCAACAAATG
    ATCTTTGAGG AACATGGCTT TAGGCGGACA ACGCCACCCA CTGCGGCCAC CCCCGTCAGG
    CTTAGGCCAA GACCATACCT GCCGAATGTA GATGAGGAGG TCCAAATCGG TCATGTTCCC
    AGGGGAGACG TAGACTACCA CCTCTATCCT CATGTTCCGG GCCTCAATCC AAATGCCTCT
    ACAGGACAGG AAGCTCTCTC TCAGACAACC ATCTCTTGGA CGCCGTTCCA GGAGAGTTCT
    GAGTACATCA TTTCATGCCA ACCAGTTGGC ACCGACGAAG AGCCCTTACA GTTCCAAGTT
    CCTGGAACTT CTACCAGTGC GACTCTGACT GGCCTTACCA GAGGGGTCAC CTACAACATC
    ATAGTGGAGG CACTGCAGAA CCAGAGGAGG CACAAGGTTC GGGAAGAGGT TGTGACTGTG
    GGCAACGCTG TCAGCGAAGG CCTGAACCAG CCTACAGATG ACTCATGCTT TGACCCTTAC
    ACGGTTTCCC ATTACGCCAT TGGAGAGGAG TGGGAGCGGT TGTCTGACGC TGGCTTTAAG
    CTCACATGCC AGTGCTTGGG CTTTGGCAGT GGTCATTTCA GATGCGATTC ATCTAAATGG
    TGCCATGACA ACGGTGTCAA CTACAAGATC GGAGAGAAGT GGGATCGGCA GGGAGAAAAT
    GGCCAGCGGA TGAGCTGCAC ATGCCTCGGG AATGGAAAGG GAGAATTCAA GTGTGATCCC
    CATGAAGCAA CGTGCTATGA CGATGGGAAG ACCTACCATG TAGGAGAACA GTGGCAGAAA
    GAATATCTCG GAGCCATTTG CTCCTGCACG TGTTTCGGAG GCCAGCGGGG CTGGCGCTGT
    GACAACTGCC GTAGACCTGG GGCTGCTGAA CCCAGTCCCG ATGGCACCAC CGGCCACACC
    TACAACCAGT ATACACAGAG ATACAATCAG AGAACAAACA CTAACGTAAA TTGCCCCATT
    GAGTGCTTCA TGCCGCTAGA TGTGCAAGCT GACAGAGACG ATTCTCGAGA GTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_034363.1
    CDS253..7686
    Translation

    Target ORF information:

    RefSeq Version NM_010233.2
    Organism Mus musculus(house mouse)
    Definition Mus musculus fibronectin 1 (Fn1), transcript variant 1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_010233.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    ATGCTCAGGG GTCCGGGACC CGGGCGGCTG CTGCTGCTGG CAGTCCTGTG CCTGGGGACC 
    TCGGTGCGCT GCACCGAAGC CGGGAAGAGC AAGAGGCAGG CTCAGCAAAT CGTGCAGCCT
    CAATCCCCGG TGGCTGTCAG TCAGAGCAAG CCTGGCTGTT TTGACAATGG GAAGCACTAT
    CAGATAAATC AGCAGTGGGA ACGGACCTAC CTAGGCAACG CCCTGGTTTG TACCTGCTAT
    GGAGGAAGCC GGGGTTTTAA CTGCGAGAGC AAGCCTGAGC CTGAAGAGAC TTGCTTTGAC
    AAATACACTG GGAACACTTA CAAAGTGGGT GACACTTATG AGCGCCCTAA AGATTCCATG
    ATCTGGGACT GTACCTGCAT CGGGGCTGGG AGAGGCAGGA TCAGCTGTAC CATTGCAAAT
    CGCTGCCATG AAGGGGGTCA GTCCTACAAG ATTGGCGACA AGTGGAGGAG GCCACATGAG
    ACTGGTGGCT ACATGTTAGA GTGTCTGTGT CTGGGAAATG GAAAAGGGGA ATGGACCTGC
    AAACCTATAG CTGAGAAGTG TTTTGATCAT GCTGCTGGGA CGTCCTACGT CGTGGGGGAG
    ACCTGGGAAA AGCCCTACCA AGGCTGGATG ATGGTGGACT GTACTTGTCT AGGCGAAGGC
    AATGGACGCA TCACCTGTAC CTCCAGAAAC AGATGCAACG ATCAGGACAC CCGGACATCC
    TATAGGATTG GAGACACGTG GAGCAAGAAG GACAACCGAG GAAACCTGCT TCAGTGTGTC
    TGCACAGGCA ATGGCAGAGG GGAGTGGAAG TGTGAGCGAC ATGCTCTACA AAGTGCTTCA
    GCCGGATCTG GCTCCTTCAC TGATGTCCGA ACAGCTATTT ACCAACCGCA GACTCACCCC
    CAGCCCGCTC CCTACGGCCA CTGTGTCACC GACAGTGGTG TGGTCTACTC TGTGGGAATG
    CAGTGGCTGA AGTCGCAAGG AAACAAGCAA ATGCTGTGCA CGTGCCTGGG CAATGGCGTC
    AGCTGCCAGG AGACAGCCGT GACCCAGACT TATGGTGGCA ATTCAAACGG GGAGCCCTGT
    GTCCTCCCGT TCACCTACAA CGGTAGGACC TTCTATTCCT GCACCACCGA AGGGCGGCAA
    GACGGACATC TGTGGTGTAG CACAACTTCC AATTACGAAC AAGACCAGAA GTATTCCTTC
    TGCACAGACC ATGCGGTTTT GGTTCAGACT CGAGGCGGAA ATTCCAATGG TGCTCTGTGC
    CACTTCCCCT TCCTGTACAA CAACCGGAAT TACACCGACT GTACTTCTGA GGGTCGCAGG
    GACAACATGA AATGGTGCGG CACCACCCAG AACTACGATG CCGATCAGAA GTTTGGATTC
    TGCCCAATGG CTGCCCACGA GGAGATCTGC ACAACCAATG AAGGGGTCAT GTATCGCATT
    GGGGATCAGT GGGATAAGCA GCATGACCTG GGCCACATGA TGAGGTGCAC GTGTGTGGGG
    AACGGTCGTG GAGAATGGGC CTGCATCCCC TACTCCCAGC TCCGAGACCA GTGCATCGTT
    GATGACATTA CTTACAATGT GAACGACACG TTCCACAAGC GTCACGAGGA GGGACATATG
    CTGAACTGTA CCTGCTTTGG TCAGGGCCGG GGCAGATGGA AGTGTGACCC CATTGACCAG
    TGCCAAGATT CAGAGACCCG GACATTTTAC CAGATTGGTG ACTCCTGGGA GAAGTTTGTG
    CATGGTGTCC GATACCAGTG TTACTGCTAC GGCCGTGGCA TCGGGGAGTG GCACTGTCAA
    CCTCTGCAGA CCTACCCAGG CACAACTGGA CCTGTCCAAG TAATTATCAC GGAGACCCCC
    AGCCAGCCCA ATTCCCACCC CATCCAGTGG AATGCCCCGG AGCCTTCACA CATCACCAAG
    TACATTCTCA GATGGAGACC TAAAACCTCT ACGGGTCGCT GGAAGGAAGC TACCATTCCT
    GGCCACCTTA ACTCCTATAC CATCAAAGGC CTGACCCCAG GTGTGATCTA TGAGGGACAG
    CTCATCAGCA TCCAGCAGTA TGGCCACAGA GAAGTGACAC GCTTCGACTT CACCACCAGC
    GCCAGCACCC CTGTGACCAG CAACACGGTG ACCGGAGAGA CTGCGCCCTA CTCTCCTGTT
    GTGGCCACTT CTGAATCTGT AACTGAAATC ACAGCCAGCA GCTTTGTGGT CTCATGGGTC
    TCAGCCTCCG ACACCGTGTC AGGCTTCCGG GTGGAGTATG AGCTGAGCGA GGAGGGAGAT
    GAACCACAGT ACCTTGATCT CCCAAGCACG GCCACTTCCG TGAACATTCC TGACCTGCTC
    CCGGGCAGAA AGTACATTGT CAATGTCTAT CAGATATCTG AGGAGGGAAA ACAGAGCTTG
    ATCCTGTCTA CCTCACAGAC TACAGCACCT GACGCTCCTC CAGACCCTAC CGTGGACCAG
    GTTGATGATA CTTCCATTGT TGTTCGGTGG AGTAGACCCC AGGCACCTAT CACAGGGTAT
    AGAATTGTCT ATTCACCTTC AGTAGAAGGC AGTAGCACAG AGCTCAACCT CCCTGAAACG
    GCCAACTCCG TCACCCTCAG CGACCTGCAG CCCGGTGTTC AGTACAACAT CACTATCTAT
    GCTGTGGAGG AGAACCAGGA GAGCACACCC GTTTTCATCC AACAAGAGAC CACTGGCACC
    CCAAGATCTG ATAACGTCCC CCCTCCGACG GACCTACAGT TTGTGGAACT GACTGATGTG
    AAAGTCACCA TCATGTGGAC CCCTCCTGAT AGTGTGGTGT CTGGATACCG TGTGGAGGTC
    CTGCCTGTCA GCCTGCCCGG GGAACATGGG CAGAGGCTGC CTGTCAACAG AAATACCTTT
    GCTGAAATCA CTGGGCTGTC CCCTGGGGTC ACGTACCTCT TCAAAGTCTT TGCTGTGCAC
    CAGGGCAGGG AAAGCAATCC TCTGACGGCA CAACAGACCA CCAAACTCGA CGCTCCCACT
    AACCTCCAGT TTGTCAATGA AACTGACAGA ACAGTTCTGG TAACGTGGAC TCCACCTCGA
    GCCCGTATAG CAGGCTACCG ACTGACCGCG GGCCTGACCC GAGGAGGCCA GCCCAAGCAG
    TACAATGTGG GACCCTTGGC CTCCAAGTAT CCCCTGAGAA ATCTGCAGCC TGGGTCTGAG
    TACACCGTGA CCTTGGTGGC TGTGAAAGGG AACCAGCAGA GTCCCAAAGC CACCGGAGTC
    TTTACTACCC TGCAGCCTCT GCGCTCCATT CCACCTTACA ACACCGAGGT GACAGAGACC
    ACAATTGTGA TCACCTGGAC CCCCGCTCCA AGGATTGGCT TCAAGCTGGG TGTACGACCG
    AGCCAGGGAG GTGAGGCACC CCGAGAAGTG ACTTCAGACT CTGGGAGCAT TGTTGTGTCT
    GGCTTGACTC CAGGCGTGGA ATACACTTAC ACCATCCAAG TCCTGCGAGA TGGCCAGGAG
    AGAGATGCAC CGATTGTCAA CAGAGTAGTG ACACCGCTGT CTCCACCGAC CAACTTGCAT
    CTGGAGGCAA ACCCTGACAC TGGAGTGCTT ACTGTCTCCT GGGAGAGGAG CACTACCCCA
    GATATCACTG GCTACAGAAT AACTACTACC CCCACGAACG GGCAGCAGGG GACCTCTCTG
    GAAGAAGTGG TCCATGCTGA TCAGAGTTCC TGCACTTTTG AGAACCTGAA TCCTGGCCTG
    GAGTACAACG TCAGTGTTTA CACTGTCAAA GATGACAAGG AAAGTGCCCC TATCTCTGAT
    ACCGTTGTCC CAGAGGTGCC CCAGCTCACT GACCTAAGCT TTGTTGATAT AACTGATTCA
    AGCATCGGCC TGAGGTGGAC CCCGCTAAAC TCTTCCACCA TTATCGGGTA CCGAATCACA
    GTAGTTGCGG CAGGAGAAGG GATCCCTATT TTTGAAGATT TTGTGGACTC CTCAGTAGGA
    TACTACACAG TTACAGGGCT GGAGCCTGGC ATTGACTATG ACATCAGCGT TATCACTCTC
    ATTAATGGCG GAGAGAGTGC CCCTACTACA CTGACACAGC AAACGGCTGT CCCTCCTCCC
    ACGGATCTGC GATTCACCAA TATCGGTCCA GACACGATGC GGGTCACTTG GGCCCCGCCT
    CCGTCCATCG AGCTGACCAA CCTCTTGGTG CGCTACTCAC CCGTGAAGAA TGAAGAGGAC
    GTTGCAGAGC TATCCATTTC ACCTTCAGAC AATGCCGTGG TCCTAACAAA TCTCCTGCCT
    GGGACAGAAT ACTTAGTCAG TGTCTCCAGT GTCTACGAAC AACATGAGAG CATCCCTCTC
    CGGGGAAGGC AGAAAACAGG TCTCGATTCC CCAACTGGTT TTGATTCTTC TGATATCACC
    GCCAACTCAT TCACTGTCCA CTGGGTGGCT CCTCGGGCCC CCATCACCGG CTACATCATC
    CGCCATCACG CCGAGCATTC TGTCGGAAGA CCCAGGCAGG ATCGAGTGCC GCCCTCGCGG
    AATTCCATCA CCCTCACCAA CCTTAATCCG GGCACCGAGT ACGTTGTCAG CATCATTGCT
    GTTAATGGCA GAGAGGAGAG CCCGCCACTG ATTGGCCAGC AAGCCACAGT TTCTGATATT
    CCGAGAGATC TGGAGGTCAT TGCCTCCACC CCCACCAGCC TGCTCATCAG TTGGGAACCC
    CCTGCCGTCT CTGTGCGCTA TTACAGAATC ACCTACGGAG AGACAGGAGG AAATAGCCCT
    GTCCAGGAGT TCACTGTGCC CGGAAGCAAG TCCACAGCCA CCATCAACAA CATTAAACCA
    GGAGCAGACT ACACCATCAC CCTGTATGCT GTCACTGGCC GTGGGGACAG TCCAGCAAGC
    AGCAAGCCAG TTTCCATCAA TTATAAAACA GAAATTGACA AGCCGTCCCA GATGCAGGTG
    ACAGATGTCC AGGACAACAG CATCAGTGTC AGGTGGCTGC CTTCAACTTC TCCTGTGACA
    GGCTACAGAG TGACCACCAC TCCCAAAAAT GGCCTAGGAC CATCAAAAAC TAAAACTGCC
    AGTCCAGATC AAACAGAAAT GACCATTGAA GGTTTGCAAC CCACTGTGGA GTACGTGGTT
    AGTGTTTATG CTCAGAACCG GAACGGAGAA AGCCAGCCCC TGGTTCAAAC TGCAGTGACC
    AACATTGATC GCCCTAAAGG ACTGGCATTC ACTGATGTGG ATGTCGATTC CATCAAAATT
    GCTTGGGAAA GCCCACAGGG GCAAGTTTCC AGGTACAGGG TGACCTACTC GAGCCCTGAG
    GATGGAATCC GGGAGCTTTT CCCTGCACCT GATGGTGAAG ACGACACTGC AGAGCTGCAG
    GGCCTCAGGC CGGGGTCTGA GTACACAGTC AGTGTGGTTG CCTTGCACGA TGATATGGAG
    AGCCAGCCCC TGATTGGAAT CCAGTCCACA GCCATTCCTG CGCCCACCAA TCTGAAGTTC
    AGTCAGGTGA CACCCACCAG CTTTACTGCC CAGTGGATAG CACCCAGTGT TCAGCTCACT
    GGCTACCGGG TGCGGGTGAA CCCGAAAGAG AAGACAGGAC CAATGAAAGA AATCAACCTT
    TCTCCAGACA GCTCATCGGT GATTGTGTCA GGACTCATGG TGGCCACTAA ATACGAAGTC
    AGTGTCTATG CTCTCAAGGA CACACTGACA AGCAGACCAG CCCAGGGAGT CATCACTACT
    CTGGAGAATG TTAGCCCTCC AAGAAGGGCC CGTGTGACGG ACGCTACAGA GACCACCATC
    ACTATTAGCT GGAGAACAAA GACAGAGACA ATCACTGGCT TCCAAGTCGA TGCCATCCCA
    GCCAATGGCC AGACCCCAGT TCAGAGGAGC ATCAGCCCGG ATGTTAGAAG CTACACCATT
    ACAGGTTTAC AGCCAGGCAC TGACTACAAG ATCCACCTGT ACACTCTGAA CGACAATGCC
    CGGAGCTCGC CCGTGATCAT CGATGCCTCC ACCGCCATCG ACGCACCATC CAACCTGCGG
    TTCCTGACCA CCACACCCAA CTCCTTGCTG GTGTCATGGC AGGCGCCCCG TGCCAGGATT
    ACTGGCTACA TTATCAAGTA TGAGAAGCCT GGATCCCCTC CCAGAGAAGT GGTCCCTCGG
    CCCCGCCCTG GTGTCACGGA GGCCACCATT ACTGGTCTGG AGCCAGGAAC CGAGTACACC
    ATCTACGTCA TTGCCCTGAA GAACAATCAG AAGAGTGAGC CCCTGATTGG GAGGAAGAAG
    ACAGATGAGC TTCCCCAACT GGTTACCCTT CCACACCCCA ATCTTCATGG ACCAGAGATC
    TTGGATGTTC CCTCCACAGT TCAAAAGACC CCCTTCATCA CCAACCCTGG GTATGACACC
    GAAAATGGTA TTCAGCTTCC TGGCACAACC CACCAGCAAC CCAGTGTTGG GCAACAAATG
    ATCTTTGAGG AACATGGCTT TAGGCGGACA ACGCCACCCA CTGCGGCCAC CCCCGTCAGG
    CTTAGGCCAA GACCATACCT GCCGAATGTA GATGAGGAGG TCCAAATCGG TCATGTTCCC
    AGGGGAGACG TAGACTACCA CCTCTATCCT CATGTTCCGG GCCTCAATCC AAATGCCTCT
    ACAGGACAGG AAGCTCTCTC TCAGACAACC ATCTCTTGGA CGCCGTTCCA GGAGAGTTCT
    GAGTACATCA TTTCATGCCA ACCAGTTGGC ACCGACGAAG AGCCCTTACA GTTCCAAGTT
    CCTGGAACTT CTACCAGTGC GACTCTGACT GGCCTTACCA GAGGGGTCAC CTACAACATC
    ATAGTGGAGG CACTGCAGAA CCAGAGGAGG CACAAGGTTC GGGAAGAGGT TGTGACTGTG
    GGCAACGCTG TCAGCGAAGG CCTGAACCAG CCTACAGATG ACTCATGCTT TGACCCTTAC
    ACGGTTTCCC ATTACGCCAT TGGAGAGGAG TGGGAGCGGT TGTCTGACGC TGGCTTTAAG
    CTCACATGCC AGTGCTTGGG CTTTGGCAGT GGTCATTTCA GATGCGATTC ATCTAAATGG
    TGCCATGACA ACGGTGTCAA CTACAAGATC GGAGAGAAGT GGGATCGGCA GGGAGAAAAT
    GGCCAGCGGA TGAGCTGCAC ATGCCTCGGG AATGGAAAGG GAGAATTCAA GTGTGATCCC
    CATGAAGCAA CGTGCTATGA CGATGGGAAG ACCTACCATG TAGGAGAACA GTGGCAGAAA
    GAATATCTCG GAGCCATTTG CTCCTGCACG TGTTTCGGAG GCCAGCGGGG CTGGCGCTGT
    GACAACTGCC GTAGACCTGG GGCTGCTGAA CCCAGTCCCG ATGGCACCAC CGGCCACACC
    TACAACCAGT ATACACAGAG ATACAATCAG AGAACAAACA CTAACGTAAA TTGCCCCATT
    GAGTGCTTCA TGCCGCTAGA TGTGCAAGCT GACAGAGACG ATTCTCGAGA GTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu23089
    Clone ID Related Accession (Same CDS sequence) NM_001276410.1
    Accession Version NM_001276410.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6891bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-24
    Organism Mus musculus(house mouse)
    Product fibronectin isoform d precursor
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from CJ098561.1, AK171339.1, CK639246.1, CN692112.1, CN536551.1, CN532664.1, AK147315.1 and AC124821.5. Transcript Variant: This variant (4) lacks two in-frame exons in the coding region, compared to variant 1. The encoded isoform (d) is shorter than isoform a. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK170755.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164134, SAMN01164135 [ECO:0000348] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    ATGCTCAGGG GTCCGGGACC CGGGCGGCTG CTGCTGCTGG CAGTCCTGTG CCTGGGGACC 
    TCGGTGCGCT GCACCGAAGC CGGGAAGAGC AAGAGGCAGG CTCAGCAAAT CGTGCAGCCT
    CAATCCCCGG TGGCTGTCAG TCAGAGCAAG CCTGGCTGTT TTGACAATGG GAAGCACTAT
    CAGATAAATC AGCAGTGGGA ACGGACCTAC CTAGGCAACG CCCTGGTTTG TACCTGCTAT
    GGAGGAAGCC GGGGTTTTAA CTGCGAGAGC AAGCCTGAGC CTGAAGAGAC TTGCTTTGAC
    AAATACACTG GGAACACTTA CAAAGTGGGT GACACTTATG AGCGCCCTAA AGATTCCATG
    ATCTGGGACT GTACCTGCAT CGGGGCTGGG AGAGGCAGGA TCAGCTGTAC CATTGCAAAT
    CGCTGCCATG AAGGGGGTCA GTCCTACAAG ATTGGCGACA AGTGGAGGAG GCCACATGAG
    ACTGGTGGCT ACATGTTAGA GTGTCTGTGT CTGGGAAATG GAAAAGGGGA ATGGACCTGC
    AAACCTATAG CTGAGAAGTG TTTTGATCAT GCTGCTGGGA CGTCCTACGT CGTGGGGGAG
    ACCTGGGAAA AGCCCTACCA AGGCTGGATG ATGGTGGACT GTACTTGTCT AGGCGAAGGC
    AATGGACGCA TCACCTGTAC CTCCAGAAAC AGATGCAACG ATCAGGACAC CCGGACATCC
    TATAGGATTG GAGACACGTG GAGCAAGAAG GACAACCGAG GAAACCTGCT TCAGTGTGTC
    TGCACAGGCA ATGGCAGAGG GGAGTGGAAG TGTGAGCGAC ATGCTCTACA AAGTGCTTCA
    GCCGGATCTG GCTCCTTCAC TGATGTCCGA ACAGCTATTT ACCAACCGCA GACTCACCCC
    CAGCCCGCTC CCTACGGCCA CTGTGTCACC GACAGTGGTG TGGTCTACTC TGTGGGAATG
    CAGTGGCTGA AGTCGCAAGG AAACAAGCAA ATGCTGTGCA CGTGCCTGGG CAATGGCGTC
    AGCTGCCAGG AGACAGCCGT GACCCAGACT TATGGTGGCA ATTCAAACGG GGAGCCCTGT
    GTCCTCCCGT TCACCTACAA CGGTAGGACC TTCTATTCCT GCACCACCGA AGGGCGGCAA
    GACGGACATC TGTGGTGTAG CACAACTTCC AATTACGAAC AAGACCAGAA GTATTCCTTC
    TGCACAGACC ATGCGGTTTT GGTTCAGACT CGAGGCGGAA ATTCCAATGG TGCTCTGTGC
    CACTTCCCCT TCCTGTACAA CAACCGGAAT TACACCGACT GTACTTCTGA GGGTCGCAGG
    GACAACATGA AATGGTGCGG CACCACCCAG AACTACGATG CCGATCAGAA GTTTGGATTC
    TGCCCAATGG CTGCCCACGA GGAGATCTGC ACAACCAATG AAGGGGTCAT GTATCGCATT
    GGGGATCAGT GGGATAAGCA GCATGACCTG GGCCACATGA TGAGGTGCAC GTGTGTGGGG
    AACGGTCGTG GAGAATGGGC CTGCATCCCC TACTCCCAGC TCCGAGACCA GTGCATCGTT
    GATGACATTA CTTACAATGT GAACGACACG TTCCACAAGC GTCACGAGGA GGGACATATG
    CTGAACTGTA CCTGCTTTGG TCAGGGCCGG GGCAGATGGA AGTGTGACCC CATTGACCAG
    TGCCAAGATT CAGAGACCCG GACATTTTAC CAGATTGGTG ACTCCTGGGA GAAGTTTGTG
    CATGGTGTCC GATACCAGTG TTACTGCTAC GGCCGTGGCA TCGGGGAGTG GCACTGTCAA
    CCTCTGCAGA CCTACCCAGG CACAACTGGA CCTGTCCAAG TAATTATCAC GGAGACCCCC
    AGCCAGCCCA ATTCCCACCC CATCCAGTGG AATGCCCCGG AGCCTTCACA CATCACCAAG
    TACATTCTCA GATGGAGACC TAAAACCTCT ACGGGTCGCT GGAAGGAAGC TACCATTCCT
    GGCCACCTTA ACTCCTATAC CATCAAAGGC CTGACCCCAG GTGTGATCTA TGAGGGACAG
    CTCATCAGCA TCCAGCAGTA TGGCCACAGA GAAGTGACAC GCTTCGACTT CACCACCAGC
    GCCAGCACCC CTGTGACCAG CAACACGGTG ACCGGAGAGA CTGCGCCCTA CTCTCCTGTT
    GTGGCCACTT CTGAATCTGT AACTGAAATC ACAGCCAGCA GCTTTGTGGT CTCATGGGTC
    TCAGCCTCCG ACACCGTGTC AGGCTTCCGG GTGGAGTATG AGCTGAGCGA GGAGGGAGAT
    GAACCACAGT ACCTTGATCT CCCAAGCACG GCCACTTCCG TGAACATTCC TGACCTGCTC
    CCGGGCAGAA AGTACATTGT CAATGTCTAT CAGATATCTG AGGAGGGAAA ACAGAGCTTG
    ATCCTGTCTA CCTCACAGAC TACAGCACCT GACGCTCCTC CAGACCCTAC CGTGGACCAG
    GTTGATGATA CTTCCATTGT TGTTCGGTGG AGTAGACCCC AGGCACCTAT CACAGGGTAT
    AGAATTGTCT ATTCACCTTC AGTAGAAGGC AGTAGCACAG AGCTCAACCT CCCTGAAACG
    GCCAACTCCG TCACCCTCAG CGACCTGCAG CCCGGTGTTC AGTACAACAT CACTATCTAT
    GCTGTGGAGG AGAACCAGGA GAGCACACCC GTTTTCATCC AACAAGAGAC CACTGGCACC
    CCAAGATCTG ATAACGTCCC CCCTCCGACG GACCTACAGT TTGTGGAACT GACTGATGTG
    AAAGTCACCA TCATGTGGAC CCCTCCTGAT AGTGTGGTGT CTGGATACCG TGTGGAGGTC
    CTGCCTGTCA GCCTGCCCGG GGAACATGGG CAGAGGCTGC CTGTCAACAG AAATACCTTT
    GCTGAAATCA CTGGGCTGTC CCCTGGGGTC ACGTACCTCT TCAAAGTCTT TGCTGTGCAC
    CAGGGCAGGG AAAGCAATCC TCTGACGGCA CAACAGACCA CCAAACTCGA CGCTCCCACT
    AACCTCCAGT TTGTCAATGA AACTGACAGA ACAGTTCTGG TAACGTGGAC TCCACCTCGA
    GCCCGTATAG CAGGCTACCG ACTGACCGCG GGCCTGACCC GAGGAGGCCA GCCCAAGCAG
    TACAATGTGG GACCCTTGGC CTCCAAGTAT CCCCTGAGAA ATCTGCAGCC TGGGTCTGAG
    TACACCGTGA CCTTGGTGGC TGTGAAAGGG AACCAGCAGA GTCCCAAAGC CACCGGAGTC
    TTTACTACCC TGCAGCCTCT GCGCTCCATT CCACCTTACA ACACCGAGGT GACAGAGACC
    ACAATTGTGA TCACCTGGAC CCCCGCTCCA AGGATTGGCT TCAAGCTGGG TGTACGACCG
    AGCCAGGGAG GTGAGGCACC CCGAGAAGTG ACTTCAGACT CTGGGAGCAT TGTTGTGTCT
    GGCTTGACTC CAGGCGTGGA ATACACTTAC ACCATCCAAG TCCTGCGAGA TGGCCAGGAG
    AGAGATGCAC CGATTGTCAA CAGAGTAGTG ACACCGCTGT CTCCACCGAC CAACTTGCAT
    CTGGAGGCAA ACCCTGACAC TGGAGTGCTT ACTGTCTCCT GGGAGAGGAG CACTACCCCA
    GATATCACTG GCTACAGAAT AACTACTACC CCCACGAACG GGCAGCAGGG GACCTCTCTG
    GAAGAAGTGG TCCATGCTGA TCAGAGTTCC TGCACTTTTG AGAACCTGAA TCCTGGCCTG
    GAGTACAACG TCAGTGTTTA CACTGTCAAA GATGACAAGG AAAGTGCCCC TATCTCTGAT
    ACCGTTGTCC CAGCTGTCCC TCCTCCCACG GATCTGCGAT TCACCAATAT CGGTCCAGAC
    ACGATGCGGG TCACTTGGGC CCCGCCTCCG TCCATCGAGC TGACCAACCT CTTGGTGCGC
    TACTCACCCG TGAAGAATGA AGAGGACGTT GCAGAGCTAT CCATTTCACC TTCAGACAAT
    GCCGTGGTCC TAACAAATCT CCTGCCTGGG ACAGAATACT TAGTCAGTGT CTCCAGTGTC
    TACGAACAAC ATGAGAGCAT CCCTCTCCGG GGAAGGCAGA AAACAGGTCT CGATTCCCCA
    ACTGGTTTTG ATTCTTCTGA TATCACCGCC AACTCATTCA CTGTCCACTG GGTGGCTCCT
    CGGGCCCCCA TCACCGGCTA CATCATCCGC CATCACGCCG AGCATTCTGT CGGAAGACCC
    AGGCAGGATC GAGTGCCGCC CTCGCGGAAT TCCATCACCC TCACCAACCT TAATCCGGGC
    ACCGAGTACG TTGTCAGCAT CATTGCTGTT AATGGCAGAG AGGAGAGCCC GCCACTGATT
    GGCCAGCAAG CCACAGTTTC TGATATTCCG AGAGATCTGG AGGTCATTGC CTCCACCCCC
    ACCAGCCTGC TCATCAGTTG GGAACCCCCT GCCGTCTCTG TGCGCTATTA CAGAATCACC
    TACGGAGAGA CAGGAGGAAA TAGCCCTGTC CAGGAGTTCA CTGTGCCCGG AAGCAAGTCC
    ACAGCCACCA TCAACAACAT TAAACCAGGA GCAGACTACA CCATCACCCT GTATGCTGTC
    ACTGGCCGTG GGGACAGTCC AGCAAGCAGC AAGCCAGTTT CCATCAATTA TAAAACAGAA
    ATTGACAAGC CGTCCCAGAT GCAGGTGACA GATGTCCAGG ACAACAGCAT CAGTGTCAGG
    TGGCTGCCTT CAACTTCTCC TGTGACAGGC TACAGAGTGA CCACCACTCC CAAAAATGGC
    CTAGGACCAT CAAAAACTAA AACTGCCAGT CCAGATCAAA CAGAAATGAC CATTGAAGGT
    TTGCAACCCA CTGTGGAGTA CGTGGTTAGT GTTTATGCTC AGAACCGGAA CGGAGAAAGC
    CAGCCCCTGG TTCAAACTGC AGTGACCACC ATTCCTGCGC CCACCAATCT GAAGTTCAGT
    CAGGTGACAC CCACCAGCTT TACTGCCCAG TGGATAGCAC CCAGTGTTCA GCTCACTGGC
    TACCGGGTGC GGGTGAACCC GAAAGAGAAG ACAGGACCAA TGAAAGAAAT CAACCTTTCT
    CCAGACAGCT CATCGGTGAT TGTGTCAGGA CTCATGGTGG CCACTAAATA CGAAGTCAGT
    GTCTATGCTC TCAAGGACAC ACTGACAAGC AGACCAGCCC AGGGAGTCAT CACTACTCTG
    GAGAATGTTA GCCCTCCAAG AAGGGCCCGT GTGACGGACG CTACAGAGAC CACCATCACT
    ATTAGCTGGA GAACAAAGAC AGAGACAATC ACTGGCTTCC AAGTCGATGC CATCCCAGCC
    AATGGCCAGA CCCCAGTTCA GAGGAGCATC AGCCCGGATG TTAGAAGCTA CACCATTACA
    GGTTTACAGC CAGGCACTGA CTACAAGATC CACCTGTACA CTCTGAACGA CAATGCCCGG
    AGCTCGCCCG TGATCATCGA TGCCTCCACC GCCATCGACG CACCATCCAA CCTGCGGTTC
    CTGACCACCA CACCCAACTC CTTGCTGGTG TCATGGCAGG CGCCCCGTGC CAGGATTACT
    GGCTACATTA TCAAGTATGA GAAGCCTGGA TCCCCTCCCA GAGAAGTGGT CCCTCGGCCC
    CGCCCTGGTG TCACGGAGGC CACCATTACT GGTCTGGAGC CAGGAACCGA GTACACCATC
    TACGTCATTG CCCTGAAGAA CAATCAGAAG AGTGAGCCCC TGATTGGGAG GAAGAAGACA
    GATGAGCTTC CCCAACTGGT TACCCTTCCA CACCCCAATC TTCATGGACC AGAGATCTTG
    GATGTTCCCT CCACAGTTCA AAAGACCCCC TTCATCACCA ACCCTGGGTA TGACACCGAA
    AATGGTATTC AGCTTCCTGG CACAACCCAC CAGCAACCCA GTGTTGGGCA ACAAATGATC
    TTTGAGGAAC ATGGCTTTAG GCGGACAACG CCACCCACTG CGGCCACCCC CGTCAGGCTT
    AGGCCAAGAC CATACCTGCC GAATGTAGAT GAGGAGGTCC AAATCGGTCA TGTTCCCAGG
    GGAGACGTAG ACTACCACCT CTATCCTCAT GTTCCGGGCC TCAATCCAAA TGCCTCTACA
    GGACAGGAAG CTCTCTCTCA GACAACCATC TCTTGGACGC CGTTCCAGGA GAGTTCTGAG
    TACATCATTT CATGCCAACC AGTTGGCACC GACGAAGAGC CCTTACAGTT CCAAGTTCCT
    GGAACTTCTA CCAGTGCGAC TCTGACTGGC CTTACCAGAG GGGTCACCTA CAACATCATA
    GTGGAGGCAC TGCAGAACCA GAGGAGGCAC AAGGTTCGGG AAGAGGTTGT GACTGTGGGC
    AACGCTGTCA GCGAAGGCCT GAACCAGCCT ACAGATGACT CATGCTTTGA CCCTTACACG
    GTTTCCCATT ACGCCATTGG AGAGGAGTGG GAGCGGTTGT CTGACGCTGG CTTTAAGCTC
    ACATGCCAGT GCTTGGGCTT TGGCAGTGGT CATTTCAGAT GCGATTCATC TAAATGGTGC
    CATGACAACG GTGTCAACTA CAAGATCGGA GAGAAGTGGG ATCGGCAGGG AGAAAATGGC
    CAGCGGATGA GCTGCACATG CCTCGGGAAT GGAAAGGGAG AATTCAAGTG TGATCCCCAT
    GAAGCAACGT GCTATGACGA TGGGAAGACC TACCATGTAG GAGAACAGTG GCAGAAAGAA
    TATCTCGGAG CCATTTGCTC CTGCACGTGT TTCGGAGGCC AGCGGGGCTG GCGCTGTGAC
    AACTGCCGTA GACCTGGGGC TGCTGAACCC AGTCCCGATG GCACCACCGG CCACACCTAC
    AACCAGTATA CACAGAGATA CAATCAGAGA ACAAACACTA ACGTAAATTG CCCCATTGAG
    TGCTTCATGC CGCTAGATGT GCAAGCTGAC AGAGACGATT CTCGAGAGTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001263339.1
    CDS253..7143
    Translation

    Target ORF information:

    RefSeq Version NM_001276410.1
    Organism Mus musculus(house mouse)
    Definition Mus musculus fibronectin 1 (Fn1), transcript variant 4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001276410.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    ATGCTCAGGG GTCCGGGACC CGGGCGGCTG CTGCTGCTGG CAGTCCTGTG CCTGGGGACC 
    TCGGTGCGCT GCACCGAAGC CGGGAAGAGC AAGAGGCAGG CTCAGCAAAT CGTGCAGCCT
    CAATCCCCGG TGGCTGTCAG TCAGAGCAAG CCTGGCTGTT TTGACAATGG GAAGCACTAT
    CAGATAAATC AGCAGTGGGA ACGGACCTAC CTAGGCAACG CCCTGGTTTG TACCTGCTAT
    GGAGGAAGCC GGGGTTTTAA CTGCGAGAGC AAGCCTGAGC CTGAAGAGAC TTGCTTTGAC
    AAATACACTG GGAACACTTA CAAAGTGGGT GACACTTATG AGCGCCCTAA AGATTCCATG
    ATCTGGGACT GTACCTGCAT CGGGGCTGGG AGAGGCAGGA TCAGCTGTAC CATTGCAAAT
    CGCTGCCATG AAGGGGGTCA GTCCTACAAG ATTGGCGACA AGTGGAGGAG GCCACATGAG
    ACTGGTGGCT ACATGTTAGA GTGTCTGTGT CTGGGAAATG GAAAAGGGGA ATGGACCTGC
    AAACCTATAG CTGAGAAGTG TTTTGATCAT GCTGCTGGGA CGTCCTACGT CGTGGGGGAG
    ACCTGGGAAA AGCCCTACCA AGGCTGGATG ATGGTGGACT GTACTTGTCT AGGCGAAGGC
    AATGGACGCA TCACCTGTAC CTCCAGAAAC AGATGCAACG ATCAGGACAC CCGGACATCC
    TATAGGATTG GAGACACGTG GAGCAAGAAG GACAACCGAG GAAACCTGCT TCAGTGTGTC
    TGCACAGGCA ATGGCAGAGG GGAGTGGAAG TGTGAGCGAC ATGCTCTACA AAGTGCTTCA
    GCCGGATCTG GCTCCTTCAC TGATGTCCGA ACAGCTATTT ACCAACCGCA GACTCACCCC
    CAGCCCGCTC CCTACGGCCA CTGTGTCACC GACAGTGGTG TGGTCTACTC TGTGGGAATG
    CAGTGGCTGA AGTCGCAAGG AAACAAGCAA ATGCTGTGCA CGTGCCTGGG CAATGGCGTC
    AGCTGCCAGG AGACAGCCGT GACCCAGACT TATGGTGGCA ATTCAAACGG GGAGCCCTGT
    GTCCTCCCGT TCACCTACAA CGGTAGGACC TTCTATTCCT GCACCACCGA AGGGCGGCAA
    GACGGACATC TGTGGTGTAG CACAACTTCC AATTACGAAC AAGACCAGAA GTATTCCTTC
    TGCACAGACC ATGCGGTTTT GGTTCAGACT CGAGGCGGAA ATTCCAATGG TGCTCTGTGC
    CACTTCCCCT TCCTGTACAA CAACCGGAAT TACACCGACT GTACTTCTGA GGGTCGCAGG
    GACAACATGA AATGGTGCGG CACCACCCAG AACTACGATG CCGATCAGAA GTTTGGATTC
    TGCCCAATGG CTGCCCACGA GGAGATCTGC ACAACCAATG AAGGGGTCAT GTATCGCATT
    GGGGATCAGT GGGATAAGCA GCATGACCTG GGCCACATGA TGAGGTGCAC GTGTGTGGGG
    AACGGTCGTG GAGAATGGGC CTGCATCCCC TACTCCCAGC TCCGAGACCA GTGCATCGTT
    GATGACATTA CTTACAATGT GAACGACACG TTCCACAAGC GTCACGAGGA GGGACATATG
    CTGAACTGTA CCTGCTTTGG TCAGGGCCGG GGCAGATGGA AGTGTGACCC CATTGACCAG
    TGCCAAGATT CAGAGACCCG GACATTTTAC CAGATTGGTG ACTCCTGGGA GAAGTTTGTG
    CATGGTGTCC GATACCAGTG TTACTGCTAC GGCCGTGGCA TCGGGGAGTG GCACTGTCAA
    CCTCTGCAGA CCTACCCAGG CACAACTGGA CCTGTCCAAG TAATTATCAC GGAGACCCCC
    AGCCAGCCCA ATTCCCACCC CATCCAGTGG AATGCCCCGG AGCCTTCACA CATCACCAAG
    TACATTCTCA GATGGAGACC TAAAACCTCT ACGGGTCGCT GGAAGGAAGC TACCATTCCT
    GGCCACCTTA ACTCCTATAC CATCAAAGGC CTGACCCCAG GTGTGATCTA TGAGGGACAG
    CTCATCAGCA TCCAGCAGTA TGGCCACAGA GAAGTGACAC GCTTCGACTT CACCACCAGC
    GCCAGCACCC CTGTGACCAG CAACACGGTG ACCGGAGAGA CTGCGCCCTA CTCTCCTGTT
    GTGGCCACTT CTGAATCTGT AACTGAAATC ACAGCCAGCA GCTTTGTGGT CTCATGGGTC
    TCAGCCTCCG ACACCGTGTC AGGCTTCCGG GTGGAGTATG AGCTGAGCGA GGAGGGAGAT
    GAACCACAGT ACCTTGATCT CCCAAGCACG GCCACTTCCG TGAACATTCC TGACCTGCTC
    CCGGGCAGAA AGTACATTGT CAATGTCTAT CAGATATCTG AGGAGGGAAA ACAGAGCTTG
    ATCCTGTCTA CCTCACAGAC TACAGCACCT GACGCTCCTC CAGACCCTAC CGTGGACCAG
    GTTGATGATA CTTCCATTGT TGTTCGGTGG AGTAGACCCC AGGCACCTAT CACAGGGTAT
    AGAATTGTCT ATTCACCTTC AGTAGAAGGC AGTAGCACAG AGCTCAACCT CCCTGAAACG
    GCCAACTCCG TCACCCTCAG CGACCTGCAG CCCGGTGTTC AGTACAACAT CACTATCTAT
    GCTGTGGAGG AGAACCAGGA GAGCACACCC GTTTTCATCC AACAAGAGAC CACTGGCACC
    CCAAGATCTG ATAACGTCCC CCCTCCGACG GACCTACAGT TTGTGGAACT GACTGATGTG
    AAAGTCACCA TCATGTGGAC CCCTCCTGAT AGTGTGGTGT CTGGATACCG TGTGGAGGTC
    CTGCCTGTCA GCCTGCCCGG GGAACATGGG CAGAGGCTGC CTGTCAACAG AAATACCTTT
    GCTGAAATCA CTGGGCTGTC CCCTGGGGTC ACGTACCTCT TCAAAGTCTT TGCTGTGCAC
    CAGGGCAGGG AAAGCAATCC TCTGACGGCA CAACAGACCA CCAAACTCGA CGCTCCCACT
    AACCTCCAGT TTGTCAATGA AACTGACAGA ACAGTTCTGG TAACGTGGAC TCCACCTCGA
    GCCCGTATAG CAGGCTACCG ACTGACCGCG GGCCTGACCC GAGGAGGCCA GCCCAAGCAG
    TACAATGTGG GACCCTTGGC CTCCAAGTAT CCCCTGAGAA ATCTGCAGCC TGGGTCTGAG
    TACACCGTGA CCTTGGTGGC TGTGAAAGGG AACCAGCAGA GTCCCAAAGC CACCGGAGTC
    TTTACTACCC TGCAGCCTCT GCGCTCCATT CCACCTTACA ACACCGAGGT GACAGAGACC
    ACAATTGTGA TCACCTGGAC CCCCGCTCCA AGGATTGGCT TCAAGCTGGG TGTACGACCG
    AGCCAGGGAG GTGAGGCACC CCGAGAAGTG ACTTCAGACT CTGGGAGCAT TGTTGTGTCT
    GGCTTGACTC CAGGCGTGGA ATACACTTAC ACCATCCAAG TCCTGCGAGA TGGCCAGGAG
    AGAGATGCAC CGATTGTCAA CAGAGTAGTG ACACCGCTGT CTCCACCGAC CAACTTGCAT
    CTGGAGGCAA ACCCTGACAC TGGAGTGCTT ACTGTCTCCT GGGAGAGGAG CACTACCCCA
    GATATCACTG GCTACAGAAT AACTACTACC CCCACGAACG GGCAGCAGGG GACCTCTCTG
    GAAGAAGTGG TCCATGCTGA TCAGAGTTCC TGCACTTTTG AGAACCTGAA TCCTGGCCTG
    GAGTACAACG TCAGTGTTTA CACTGTCAAA GATGACAAGG AAAGTGCCCC TATCTCTGAT
    ACCGTTGTCC CAGCTGTCCC TCCTCCCACG GATCTGCGAT TCACCAATAT CGGTCCAGAC
    ACGATGCGGG TCACTTGGGC CCCGCCTCCG TCCATCGAGC TGACCAACCT CTTGGTGCGC
    TACTCACCCG TGAAGAATGA AGAGGACGTT GCAGAGCTAT CCATTTCACC TTCAGACAAT
    GCCGTGGTCC TAACAAATCT CCTGCCTGGG ACAGAATACT TAGTCAGTGT CTCCAGTGTC
    TACGAACAAC ATGAGAGCAT CCCTCTCCGG GGAAGGCAGA AAACAGGTCT CGATTCCCCA
    ACTGGTTTTG ATTCTTCTGA TATCACCGCC AACTCATTCA CTGTCCACTG GGTGGCTCCT
    CGGGCCCCCA TCACCGGCTA CATCATCCGC CATCACGCCG AGCATTCTGT CGGAAGACCC
    AGGCAGGATC GAGTGCCGCC CTCGCGGAAT TCCATCACCC TCACCAACCT TAATCCGGGC
    ACCGAGTACG TTGTCAGCAT CATTGCTGTT AATGGCAGAG AGGAGAGCCC GCCACTGATT
    GGCCAGCAAG CCACAGTTTC TGATATTCCG AGAGATCTGG AGGTCATTGC CTCCACCCCC
    ACCAGCCTGC TCATCAGTTG GGAACCCCCT GCCGTCTCTG TGCGCTATTA CAGAATCACC
    TACGGAGAGA CAGGAGGAAA TAGCCCTGTC CAGGAGTTCA CTGTGCCCGG AAGCAAGTCC
    ACAGCCACCA TCAACAACAT TAAACCAGGA GCAGACTACA CCATCACCCT GTATGCTGTC
    ACTGGCCGTG GGGACAGTCC AGCAAGCAGC AAGCCAGTTT CCATCAATTA TAAAACAGAA
    ATTGACAAGC CGTCCCAGAT GCAGGTGACA GATGTCCAGG ACAACAGCAT CAGTGTCAGG
    TGGCTGCCTT CAACTTCTCC TGTGACAGGC TACAGAGTGA CCACCACTCC CAAAAATGGC
    CTAGGACCAT CAAAAACTAA AACTGCCAGT CCAGATCAAA CAGAAATGAC CATTGAAGGT
    TTGCAACCCA CTGTGGAGTA CGTGGTTAGT GTTTATGCTC AGAACCGGAA CGGAGAAAGC
    CAGCCCCTGG TTCAAACTGC AGTGACCACC ATTCCTGCGC CCACCAATCT GAAGTTCAGT
    CAGGTGACAC CCACCAGCTT TACTGCCCAG TGGATAGCAC CCAGTGTTCA GCTCACTGGC
    TACCGGGTGC GGGTGAACCC GAAAGAGAAG ACAGGACCAA TGAAAGAAAT CAACCTTTCT
    CCAGACAGCT CATCGGTGAT TGTGTCAGGA CTCATGGTGG CCACTAAATA CGAAGTCAGT
    GTCTATGCTC TCAAGGACAC ACTGACAAGC AGACCAGCCC AGGGAGTCAT CACTACTCTG
    GAGAATGTTA GCCCTCCAAG AAGGGCCCGT GTGACGGACG CTACAGAGAC CACCATCACT
    ATTAGCTGGA GAACAAAGAC AGAGACAATC ACTGGCTTCC AAGTCGATGC CATCCCAGCC
    AATGGCCAGA CCCCAGTTCA GAGGAGCATC AGCCCGGATG TTAGAAGCTA CACCATTACA
    GGTTTACAGC CAGGCACTGA CTACAAGATC CACCTGTACA CTCTGAACGA CAATGCCCGG
    AGCTCGCCCG TGATCATCGA TGCCTCCACC GCCATCGACG CACCATCCAA CCTGCGGTTC
    CTGACCACCA CACCCAACTC CTTGCTGGTG TCATGGCAGG CGCCCCGTGC CAGGATTACT
    GGCTACATTA TCAAGTATGA GAAGCCTGGA TCCCCTCCCA GAGAAGTGGT CCCTCGGCCC
    CGCCCTGGTG TCACGGAGGC CACCATTACT GGTCTGGAGC CAGGAACCGA GTACACCATC
    TACGTCATTG CCCTGAAGAA CAATCAGAAG AGTGAGCCCC TGATTGGGAG GAAGAAGACA
    GATGAGCTTC CCCAACTGGT TACCCTTCCA CACCCCAATC TTCATGGACC AGAGATCTTG
    GATGTTCCCT CCACAGTTCA AAAGACCCCC TTCATCACCA ACCCTGGGTA TGACACCGAA
    AATGGTATTC AGCTTCCTGG CACAACCCAC CAGCAACCCA GTGTTGGGCA ACAAATGATC
    TTTGAGGAAC ATGGCTTTAG GCGGACAACG CCACCCACTG CGGCCACCCC CGTCAGGCTT
    AGGCCAAGAC CATACCTGCC GAATGTAGAT GAGGAGGTCC AAATCGGTCA TGTTCCCAGG
    GGAGACGTAG ACTACCACCT CTATCCTCAT GTTCCGGGCC TCAATCCAAA TGCCTCTACA
    GGACAGGAAG CTCTCTCTCA GACAACCATC TCTTGGACGC CGTTCCAGGA GAGTTCTGAG
    TACATCATTT CATGCCAACC AGTTGGCACC GACGAAGAGC CCTTACAGTT CCAAGTTCCT
    GGAACTTCTA CCAGTGCGAC TCTGACTGGC CTTACCAGAG GGGTCACCTA CAACATCATA
    GTGGAGGCAC TGCAGAACCA GAGGAGGCAC AAGGTTCGGG AAGAGGTTGT GACTGTGGGC
    AACGCTGTCA GCGAAGGCCT GAACCAGCCT ACAGATGACT CATGCTTTGA CCCTTACACG
    GTTTCCCATT ACGCCATTGG AGAGGAGTGG GAGCGGTTGT CTGACGCTGG CTTTAAGCTC
    ACATGCCAGT GCTTGGGCTT TGGCAGTGGT CATTTCAGAT GCGATTCATC TAAATGGTGC
    CATGACAACG GTGTCAACTA CAAGATCGGA GAGAAGTGGG ATCGGCAGGG AGAAAATGGC
    CAGCGGATGA GCTGCACATG CCTCGGGAAT GGAAAGGGAG AATTCAAGTG TGATCCCCAT
    GAAGCAACGT GCTATGACGA TGGGAAGACC TACCATGTAG GAGAACAGTG GCAGAAAGAA
    TATCTCGGAG CCATTTGCTC CTGCACGTGT TTCGGAGGCC AGCGGGGCTG GCGCTGTGAC
    AACTGCCGTA GACCTGGGGC TGCTGAACCC AGTCCCGATG GCACCACCGG CCACACCTAC
    AACCAGTATA CACAGAGATA CAATCAGAGA ACAAACACTA ACGTAAATTG CCCCATTGAG
    TGCTTCATGC CGCTAGATGT GCAAGCTGAC AGAGACGATT CTCGAGAGTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu23188
    Clone ID Related Accession (Same CDS sequence) NM_001276411.1
    Accession Version NM_001276411.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6816bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-24
    Organism Mus musculus(house mouse)
    Product fibronectin isoform e precursor
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from CJ098561.1, BC138421.1, AK147663.1 and AC124821.5. Transcript Variant: This variant (5) lacks two in-frame exons and uses an alternate in-frame splice site in the coding region, compared to variant 1. The encoded isoform (e) is shorter than isoform a. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK147663.1, BC138421.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164134, SAMN01164135 [ECO:0000348] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    ATGCTCAGGG GTCCGGGACC CGGGCGGCTG CTGCTGCTGG CAGTCCTGTG CCTGGGGACC 
    TCGGTGCGCT GCACCGAAGC CGGGAAGAGC AAGAGGCAGG CTCAGCAAAT CGTGCAGCCT
    CAATCCCCGG TGGCTGTCAG TCAGAGCAAG CCTGGCTGTT TTGACAATGG GAAGCACTAT
    CAGATAAATC AGCAGTGGGA ACGGACCTAC CTAGGCAACG CCCTGGTTTG TACCTGCTAT
    GGAGGAAGCC GGGGTTTTAA CTGCGAGAGC AAGCCTGAGC CTGAAGAGAC TTGCTTTGAC
    AAATACACTG GGAACACTTA CAAAGTGGGT GACACTTATG AGCGCCCTAA AGATTCCATG
    ATCTGGGACT GTACCTGCAT CGGGGCTGGG AGAGGCAGGA TCAGCTGTAC CATTGCAAAT
    CGCTGCCATG AAGGGGGTCA GTCCTACAAG ATTGGCGACA AGTGGAGGAG GCCACATGAG
    ACTGGTGGCT ACATGTTAGA GTGTCTGTGT CTGGGAAATG GAAAAGGGGA ATGGACCTGC
    AAACCTATAG CTGAGAAGTG TTTTGATCAT GCTGCTGGGA CGTCCTACGT CGTGGGGGAG
    ACCTGGGAAA AGCCCTACCA AGGCTGGATG ATGGTGGACT GTACTTGTCT AGGCGAAGGC
    AATGGACGCA TCACCTGTAC CTCCAGAAAC AGATGCAACG ATCAGGACAC CCGGACATCC
    TATAGGATTG GAGACACGTG GAGCAAGAAG GACAACCGAG GAAACCTGCT TCAGTGTGTC
    TGCACAGGCA ATGGCAGAGG GGAGTGGAAG TGTGAGCGAC ATGCTCTACA AAGTGCTTCA
    GCCGGATCTG GCTCCTTCAC TGATGTCCGA ACAGCTATTT ACCAACCGCA GACTCACCCC
    CAGCCCGCTC CCTACGGCCA CTGTGTCACC GACAGTGGTG TGGTCTACTC TGTGGGAATG
    CAGTGGCTGA AGTCGCAAGG AAACAAGCAA ATGCTGTGCA CGTGCCTGGG CAATGGCGTC
    AGCTGCCAGG AGACAGCCGT GACCCAGACT TATGGTGGCA ATTCAAACGG GGAGCCCTGT
    GTCCTCCCGT TCACCTACAA CGGTAGGACC TTCTATTCCT GCACCACCGA AGGGCGGCAA
    GACGGACATC TGTGGTGTAG CACAACTTCC AATTACGAAC AAGACCAGAA GTATTCCTTC
    TGCACAGACC ATGCGGTTTT GGTTCAGACT CGAGGCGGAA ATTCCAATGG TGCTCTGTGC
    CACTTCCCCT TCCTGTACAA CAACCGGAAT TACACCGACT GTACTTCTGA GGGTCGCAGG
    GACAACATGA AATGGTGCGG CACCACCCAG AACTACGATG CCGATCAGAA GTTTGGATTC
    TGCCCAATGG CTGCCCACGA GGAGATCTGC ACAACCAATG AAGGGGTCAT GTATCGCATT
    GGGGATCAGT GGGATAAGCA GCATGACCTG GGCCACATGA TGAGGTGCAC GTGTGTGGGG
    AACGGTCGTG GAGAATGGGC CTGCATCCCC TACTCCCAGC TCCGAGACCA GTGCATCGTT
    GATGACATTA CTTACAATGT GAACGACACG TTCCACAAGC GTCACGAGGA GGGACATATG
    CTGAACTGTA CCTGCTTTGG TCAGGGCCGG GGCAGATGGA AGTGTGACCC CATTGACCAG
    TGCCAAGATT CAGAGACCCG GACATTTTAC CAGATTGGTG ACTCCTGGGA GAAGTTTGTG
    CATGGTGTCC GATACCAGTG TTACTGCTAC GGCCGTGGCA TCGGGGAGTG GCACTGTCAA
    CCTCTGCAGA CCTACCCAGG CACAACTGGA CCTGTCCAAG TAATTATCAC GGAGACCCCC
    AGCCAGCCCA ATTCCCACCC CATCCAGTGG AATGCCCCGG AGCCTTCACA CATCACCAAG
    TACATTCTCA GATGGAGACC TAAAACCTCT ACGGGTCGCT GGAAGGAAGC TACCATTCCT
    GGCCACCTTA ACTCCTATAC CATCAAAGGC CTGACCCCAG GTGTGATCTA TGAGGGACAG
    CTCATCAGCA TCCAGCAGTA TGGCCACAGA GAAGTGACAC GCTTCGACTT CACCACCAGC
    GCCAGCACCC CTGTGACCAG CAACACGGTG ACCGGAGAGA CTGCGCCCTA CTCTCCTGTT
    GTGGCCACTT CTGAATCTGT AACTGAAATC ACAGCCAGCA GCTTTGTGGT CTCATGGGTC
    TCAGCCTCCG ACACCGTGTC AGGCTTCCGG GTGGAGTATG AGCTGAGCGA GGAGGGAGAT
    GAACCACAGT ACCTTGATCT CCCAAGCACG GCCACTTCCG TGAACATTCC TGACCTGCTC
    CCGGGCAGAA AGTACATTGT CAATGTCTAT CAGATATCTG AGGAGGGAAA ACAGAGCTTG
    ATCCTGTCTA CCTCACAGAC TACAGCACCT GACGCTCCTC CAGACCCTAC CGTGGACCAG
    GTTGATGATA CTTCCATTGT TGTTCGGTGG AGTAGACCCC AGGCACCTAT CACAGGGTAT
    AGAATTGTCT ATTCACCTTC AGTAGAAGGC AGTAGCACAG AGCTCAACCT CCCTGAAACG
    GCCAACTCCG TCACCCTCAG CGACCTGCAG CCCGGTGTTC AGTACAACAT CACTATCTAT
    GCTGTGGAGG AGAACCAGGA GAGCACACCC GTTTTCATCC AACAAGAGAC CACTGGCACC
    CCAAGATCTG ATAACGTCCC CCCTCCGACG GACCTACAGT TTGTGGAACT GACTGATGTG
    AAAGTCACCA TCATGTGGAC CCCTCCTGAT AGTGTGGTGT CTGGATACCG TGTGGAGGTC
    CTGCCTGTCA GCCTGCCCGG GGAACATGGG CAGAGGCTGC CTGTCAACAG AAATACCTTT
    GCTGAAATCA CTGGGCTGTC CCCTGGGGTC ACGTACCTCT TCAAAGTCTT TGCTGTGCAC
    CAGGGCAGGG AAAGCAATCC TCTGACGGCA CAACAGACCA CCAAACTCGA CGCTCCCACT
    AACCTCCAGT TTGTCAATGA AACTGACAGA ACAGTTCTGG TAACGTGGAC TCCACCTCGA
    GCCCGTATAG CAGGCTACCG ACTGACCGCG GGCCTGACCC GAGGAGGCCA GCCCAAGCAG
    TACAATGTGG GACCCTTGGC CTCCAAGTAT CCCCTGAGAA ATCTGCAGCC TGGGTCTGAG
    TACACCGTGA CCTTGGTGGC TGTGAAAGGG AACCAGCAGA GTCCCAAAGC CACCGGAGTC
    TTTACTACCC TGCAGCCTCT GCGCTCCATT CCACCTTACA ACACCGAGGT GACAGAGACC
    ACAATTGTGA TCACCTGGAC CCCCGCTCCA AGGATTGGCT TCAAGCTGGG TGTACGACCG
    AGCCAGGGAG GTGAGGCACC CCGAGAAGTG ACTTCAGACT CTGGGAGCAT TGTTGTGTCT
    GGCTTGACTC CAGGCGTGGA ATACACTTAC ACCATCCAAG TCCTGCGAGA TGGCCAGGAG
    AGAGATGCAC CGATTGTCAA CAGAGTAGTG ACACCGCTGT CTCCACCGAC CAACTTGCAT
    CTGGAGGCAA ACCCTGACAC TGGAGTGCTT ACTGTCTCCT GGGAGAGGAG CACTACCCCA
    GATATCACTG GCTACAGAAT AACTACTACC CCCACGAACG GGCAGCAGGG GACCTCTCTG
    GAAGAAGTGG TCCATGCTGA TCAGAGTTCC TGCACTTTTG AGAACCTGAA TCCTGGCCTG
    GAGTACAACG TCAGTGTTTA CACTGTCAAA GATGACAAGG AAAGTGCCCC TATCTCTGAT
    ACCGTTGTCC CAGCTGTCCC TCCTCCCACG GATCTGCGAT TCACCAATAT CGGTCCAGAC
    ACGATGCGGG TCACTTGGGC CCCGCCTCCG TCCATCGAGC TGACCAACCT CTTGGTGCGC
    TACTCACCCG TGAAGAATGA AGAGGACGTT GCAGAGCTAT CCATTTCACC TTCAGACAAT
    GCCGTGGTCC TAACAAATCT CCTGCCTGGG ACAGAATACT TAGTCAGTGT CTCCAGTGTC
    TACGAACAAC ATGAGAGCAT CCCTCTCCGG GGAAGGCAGA AAACAGGTCT CGATTCCCCA
    ACTGGTTTTG ATTCTTCTGA TATCACCGCC AACTCATTCA CTGTCCACTG GGTGGCTCCT
    CGGGCCCCCA TCACCGGCTA CATCATCCGC CATCACGCCG AGCATTCTGT CGGAAGACCC
    AGGCAGGATC GAGTGCCGCC CTCGCGGAAT TCCATCACCC TCACCAACCT TAATCCGGGC
    ACCGAGTACG TTGTCAGCAT CATTGCTGTT AATGGCAGAG AGGAGAGCCC GCCACTGATT
    GGCCAGCAAG CCACAGTTTC TGATATTCCG AGAGATCTGG AGGTCATTGC CTCCACCCCC
    ACCAGCCTGC TCATCAGTTG GGAACCCCCT GCCGTCTCTG TGCGCTATTA CAGAATCACC
    TACGGAGAGA CAGGAGGAAA TAGCCCTGTC CAGGAGTTCA CTGTGCCCGG AAGCAAGTCC
    ACAGCCACCA TCAACAACAT TAAACCAGGA GCAGACTACA CCATCACCCT GTATGCTGTC
    ACTGGCCGTG GGGACAGTCC AGCAAGCAGC AAGCCAGTTT CCATCAATTA TAAAACAGAA
    ATTGACAAGC CGTCCCAGAT GCAGGTGACA GATGTCCAGG ACAACAGCAT CAGTGTCAGG
    TGGCTGCCTT CAACTTCTCC TGTGACAGGC TACAGAGTGA CCACCACTCC CAAAAATGGC
    CTAGGACCAT CAAAAACTAA AACTGCCAGT CCAGATCAAA CAGAAATGAC CATTGAAGGT
    TTGCAACCCA CTGTGGAGTA CGTGGTTAGT GTTTATGCTC AGAACCGGAA CGGAGAAAGC
    CAGCCCCTGG TTCAAACTGC AGTGACCACC ATTCCTGCGC CCACCAATCT GAAGTTCAGT
    CAGGTGACAC CCACCAGCTT TACTGCCCAG TGGATAGCAC CCAGTGTTCA GCTCACTGGC
    TACCGGGTGC GGGTGAACCC GAAAGAGAAG ACAGGACCAA TGAAAGAAAT CAACCTTTCT
    CCAGACAGCT CATCGGTGAT TGTGTCAGGA CTCATGGTGG CCACTAAATA CGAAGTCAGT
    GTCTATGCTC TCAAGGACAC ACTGACAAGC AGACCAGCCC AGGGAGTCAT CACTACTCTG
    GAGAATGTTA GCCCTCCAAG AAGGGCCCGT GTGACGGACG CTACAGAGAC CACCATCACT
    ATTAGCTGGA GAACAAAGAC AGAGACAATC ACTGGCTTCC AAGTCGATGC CATCCCAGCC
    AATGGCCAGA CCCCAGTTCA GAGGAGCATC AGCCCGGATG TTAGAAGCTA CACCATTACA
    GGTTTACAGC CAGGCACTGA CTACAAGATC CACCTGTACA CTCTGAACGA CAATGCCCGG
    AGCTCGCCCG TGATCATCGA TGCCTCCACC GCCATCGACG CACCATCCAA CCTGCGGTTC
    CTGACCACCA CACCCAACTC CTTGCTGGTG TCATGGCAGG CGCCCCGTGC CAGGATTACT
    GGCTACATTA TCAAGTATGA GAAGCCTGGA TCCCCTCCCA GAGAAGTGGT CCCTCGGCCC
    CGCCCTGGTG TCACGGAGGC CACCATTACT GGTCTGGAGC CAGGAACCGA GTACACCATC
    TACGTCATTG CCCTGAAGAA CAATCAGAAG AGTGAGCCCC TGATTGGGAG GAAGAAGACA
    GTTCAAAAGA CCCCCTTCAT CACCAACCCT GGGTATGACA CCGAAAATGG TATTCAGCTT
    CCTGGCACAA CCCACCAGCA ACCCAGTGTT GGGCAACAAA TGATCTTTGA GGAACATGGC
    TTTAGGCGGA CAACGCCACC CACTGCGGCC ACCCCCGTCA GGCTTAGGCC AAGACCATAC
    CTGCCGAATG TAGATGAGGA GGTCCAAATC GGTCATGTTC CCAGGGGAGA CGTAGACTAC
    CACCTCTATC CTCATGTTCC GGGCCTCAAT CCAAATGCCT CTACAGGACA GGAAGCTCTC
    TCTCAGACAA CCATCTCTTG GACGCCGTTC CAGGAGAGTT CTGAGTACAT CATTTCATGC
    CAACCAGTTG GCACCGACGA AGAGCCCTTA CAGTTCCAAG TTCCTGGAAC TTCTACCAGT
    GCGACTCTGA CTGGCCTTAC CAGAGGGGTC ACCTACAACA TCATAGTGGA GGCACTGCAG
    AACCAGAGGA GGCACAAGGT TCGGGAAGAG GTTGTGACTG TGGGCAACGC TGTCAGCGAA
    GGCCTGAACC AGCCTACAGA TGACTCATGC TTTGACCCTT ACACGGTTTC CCATTACGCC
    ATTGGAGAGG AGTGGGAGCG GTTGTCTGAC GCTGGCTTTA AGCTCACATG CCAGTGCTTG
    GGCTTTGGCA GTGGTCATTT CAGATGCGAT TCATCTAAAT GGTGCCATGA CAACGGTGTC
    AACTACAAGA TCGGAGAGAA GTGGGATCGG CAGGGAGAAA ATGGCCAGCG GATGAGCTGC
    ACATGCCTCG GGAATGGAAA GGGAGAATTC AAGTGTGATC CCCATGAAGC AACGTGCTAT
    GACGATGGGA AGACCTACCA TGTAGGAGAA CAGTGGCAGA AAGAATATCT CGGAGCCATT
    TGCTCCTGCA CGTGTTTCGG AGGCCAGCGG GGCTGGCGCT GTGACAACTG CCGTAGACCT
    GGGGCTGCTG AACCCAGTCC CGATGGCACC ACCGGCCACA CCTACAACCA GTATACACAG
    AGATACAATC AGAGAACAAA CACTAACGTA AATTGCCCCA TTGAGTGCTT CATGCCGCTA
    GATGTGCAAG CTGACAGAGA CGATTCTCGA GAGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001263340.1
    CDS253..7068
    Translation

    Target ORF information:

    RefSeq Version NM_001276411.1
    Organism Mus musculus(house mouse)
    Definition Mus musculus fibronectin 1 (Fn1), transcript variant 5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001276411.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    ATGCTCAGGG GTCCGGGACC CGGGCGGCTG CTGCTGCTGG CAGTCCTGTG CCTGGGGACC 
    TCGGTGCGCT GCACCGAAGC CGGGAAGAGC AAGAGGCAGG CTCAGCAAAT CGTGCAGCCT
    CAATCCCCGG TGGCTGTCAG TCAGAGCAAG CCTGGCTGTT TTGACAATGG GAAGCACTAT
    CAGATAAATC AGCAGTGGGA ACGGACCTAC CTAGGCAACG CCCTGGTTTG TACCTGCTAT
    GGAGGAAGCC GGGGTTTTAA CTGCGAGAGC AAGCCTGAGC CTGAAGAGAC TTGCTTTGAC
    AAATACACTG GGAACACTTA CAAAGTGGGT GACACTTATG AGCGCCCTAA AGATTCCATG
    ATCTGGGACT GTACCTGCAT CGGGGCTGGG AGAGGCAGGA TCAGCTGTAC CATTGCAAAT
    CGCTGCCATG AAGGGGGTCA GTCCTACAAG ATTGGCGACA AGTGGAGGAG GCCACATGAG
    ACTGGTGGCT ACATGTTAGA GTGTCTGTGT CTGGGAAATG GAAAAGGGGA ATGGACCTGC
    AAACCTATAG CTGAGAAGTG TTTTGATCAT GCTGCTGGGA CGTCCTACGT CGTGGGGGAG
    ACCTGGGAAA AGCCCTACCA AGGCTGGATG ATGGTGGACT GTACTTGTCT AGGCGAAGGC
    AATGGACGCA TCACCTGTAC CTCCAGAAAC AGATGCAACG ATCAGGACAC CCGGACATCC
    TATAGGATTG GAGACACGTG GAGCAAGAAG GACAACCGAG GAAACCTGCT TCAGTGTGTC
    TGCACAGGCA ATGGCAGAGG GGAGTGGAAG TGTGAGCGAC ATGCTCTACA AAGTGCTTCA
    GCCGGATCTG GCTCCTTCAC TGATGTCCGA ACAGCTATTT ACCAACCGCA GACTCACCCC
    CAGCCCGCTC CCTACGGCCA CTGTGTCACC GACAGTGGTG TGGTCTACTC TGTGGGAATG
    CAGTGGCTGA AGTCGCAAGG AAACAAGCAA ATGCTGTGCA CGTGCCTGGG CAATGGCGTC
    AGCTGCCAGG AGACAGCCGT GACCCAGACT TATGGTGGCA ATTCAAACGG GGAGCCCTGT
    GTCCTCCCGT TCACCTACAA CGGTAGGACC TTCTATTCCT GCACCACCGA AGGGCGGCAA
    GACGGACATC TGTGGTGTAG CACAACTTCC AATTACGAAC AAGACCAGAA GTATTCCTTC
    TGCACAGACC ATGCGGTTTT GGTTCAGACT CGAGGCGGAA ATTCCAATGG TGCTCTGTGC
    CACTTCCCCT TCCTGTACAA CAACCGGAAT TACACCGACT GTACTTCTGA GGGTCGCAGG
    GACAACATGA AATGGTGCGG CACCACCCAG AACTACGATG CCGATCAGAA GTTTGGATTC
    TGCCCAATGG CTGCCCACGA GGAGATCTGC ACAACCAATG AAGGGGTCAT GTATCGCATT
    GGGGATCAGT GGGATAAGCA GCATGACCTG GGCCACATGA TGAGGTGCAC GTGTGTGGGG
    AACGGTCGTG GAGAATGGGC CTGCATCCCC TACTCCCAGC TCCGAGACCA GTGCATCGTT
    GATGACATTA CTTACAATGT GAACGACACG TTCCACAAGC GTCACGAGGA GGGACATATG
    CTGAACTGTA CCTGCTTTGG TCAGGGCCGG GGCAGATGGA AGTGTGACCC CATTGACCAG
    TGCCAAGATT CAGAGACCCG GACATTTTAC CAGATTGGTG ACTCCTGGGA GAAGTTTGTG
    CATGGTGTCC GATACCAGTG TTACTGCTAC GGCCGTGGCA TCGGGGAGTG GCACTGTCAA
    CCTCTGCAGA CCTACCCAGG CACAACTGGA CCTGTCCAAG TAATTATCAC GGAGACCCCC
    AGCCAGCCCA ATTCCCACCC CATCCAGTGG AATGCCCCGG AGCCTTCACA CATCACCAAG
    TACATTCTCA GATGGAGACC TAAAACCTCT ACGGGTCGCT GGAAGGAAGC TACCATTCCT
    GGCCACCTTA ACTCCTATAC CATCAAAGGC CTGACCCCAG GTGTGATCTA TGAGGGACAG
    CTCATCAGCA TCCAGCAGTA TGGCCACAGA GAAGTGACAC GCTTCGACTT CACCACCAGC
    GCCAGCACCC CTGTGACCAG CAACACGGTG ACCGGAGAGA CTGCGCCCTA CTCTCCTGTT
    GTGGCCACTT CTGAATCTGT AACTGAAATC ACAGCCAGCA GCTTTGTGGT CTCATGGGTC
    TCAGCCTCCG ACACCGTGTC AGGCTTCCGG GTGGAGTATG AGCTGAGCGA GGAGGGAGAT
    GAACCACAGT ACCTTGATCT CCCAAGCACG GCCACTTCCG TGAACATTCC TGACCTGCTC
    CCGGGCAGAA AGTACATTGT CAATGTCTAT CAGATATCTG AGGAGGGAAA ACAGAGCTTG
    ATCCTGTCTA CCTCACAGAC TACAGCACCT GACGCTCCTC CAGACCCTAC CGTGGACCAG
    GTTGATGATA CTTCCATTGT TGTTCGGTGG AGTAGACCCC AGGCACCTAT CACAGGGTAT
    AGAATTGTCT ATTCACCTTC AGTAGAAGGC AGTAGCACAG AGCTCAACCT CCCTGAAACG
    GCCAACTCCG TCACCCTCAG CGACCTGCAG CCCGGTGTTC AGTACAACAT CACTATCTAT
    GCTGTGGAGG AGAACCAGGA GAGCACACCC GTTTTCATCC AACAAGAGAC CACTGGCACC
    CCAAGATCTG ATAACGTCCC CCCTCCGACG GACCTACAGT TTGTGGAACT GACTGATGTG
    AAAGTCACCA TCATGTGGAC CCCTCCTGAT AGTGTGGTGT CTGGATACCG TGTGGAGGTC
    CTGCCTGTCA GCCTGCCCGG GGAACATGGG CAGAGGCTGC CTGTCAACAG AAATACCTTT
    GCTGAAATCA CTGGGCTGTC CCCTGGGGTC ACGTACCTCT TCAAAGTCTT TGCTGTGCAC
    CAGGGCAGGG AAAGCAATCC TCTGACGGCA CAACAGACCA CCAAACTCGA CGCTCCCACT
    AACCTCCAGT TTGTCAATGA AACTGACAGA ACAGTTCTGG TAACGTGGAC TCCACCTCGA
    GCCCGTATAG CAGGCTACCG ACTGACCGCG GGCCTGACCC GAGGAGGCCA GCCCAAGCAG
    TACAATGTGG GACCCTTGGC CTCCAAGTAT CCCCTGAGAA ATCTGCAGCC TGGGTCTGAG
    TACACCGTGA CCTTGGTGGC TGTGAAAGGG AACCAGCAGA GTCCCAAAGC CACCGGAGTC
    TTTACTACCC TGCAGCCTCT GCGCTCCATT CCACCTTACA ACACCGAGGT GACAGAGACC
    ACAATTGTGA TCACCTGGAC CCCCGCTCCA AGGATTGGCT TCAAGCTGGG TGTACGACCG
    AGCCAGGGAG GTGAGGCACC CCGAGAAGTG ACTTCAGACT CTGGGAGCAT TGTTGTGTCT
    GGCTTGACTC CAGGCGTGGA ATACACTTAC ACCATCCAAG TCCTGCGAGA TGGCCAGGAG
    AGAGATGCAC CGATTGTCAA CAGAGTAGTG ACACCGCTGT CTCCACCGAC CAACTTGCAT
    CTGGAGGCAA ACCCTGACAC TGGAGTGCTT ACTGTCTCCT GGGAGAGGAG CACTACCCCA
    GATATCACTG GCTACAGAAT AACTACTACC CCCACGAACG GGCAGCAGGG GACCTCTCTG
    GAAGAAGTGG TCCATGCTGA TCAGAGTTCC TGCACTTTTG AGAACCTGAA TCCTGGCCTG
    GAGTACAACG TCAGTGTTTA CACTGTCAAA GATGACAAGG AAAGTGCCCC TATCTCTGAT
    ACCGTTGTCC CAGCTGTCCC TCCTCCCACG GATCTGCGAT TCACCAATAT CGGTCCAGAC
    ACGATGCGGG TCACTTGGGC CCCGCCTCCG TCCATCGAGC TGACCAACCT CTTGGTGCGC
    TACTCACCCG TGAAGAATGA AGAGGACGTT GCAGAGCTAT CCATTTCACC TTCAGACAAT
    GCCGTGGTCC TAACAAATCT CCTGCCTGGG ACAGAATACT TAGTCAGTGT CTCCAGTGTC
    TACGAACAAC ATGAGAGCAT CCCTCTCCGG GGAAGGCAGA AAACAGGTCT CGATTCCCCA
    ACTGGTTTTG ATTCTTCTGA TATCACCGCC AACTCATTCA CTGTCCACTG GGTGGCTCCT
    CGGGCCCCCA TCACCGGCTA CATCATCCGC CATCACGCCG AGCATTCTGT CGGAAGACCC
    AGGCAGGATC GAGTGCCGCC CTCGCGGAAT TCCATCACCC TCACCAACCT TAATCCGGGC
    ACCGAGTACG TTGTCAGCAT CATTGCTGTT AATGGCAGAG AGGAGAGCCC GCCACTGATT
    GGCCAGCAAG CCACAGTTTC TGATATTCCG AGAGATCTGG AGGTCATTGC CTCCACCCCC
    ACCAGCCTGC TCATCAGTTG GGAACCCCCT GCCGTCTCTG TGCGCTATTA CAGAATCACC
    TACGGAGAGA CAGGAGGAAA TAGCCCTGTC CAGGAGTTCA CTGTGCCCGG AAGCAAGTCC
    ACAGCCACCA TCAACAACAT TAAACCAGGA GCAGACTACA CCATCACCCT GTATGCTGTC
    ACTGGCCGTG GGGACAGTCC AGCAAGCAGC AAGCCAGTTT CCATCAATTA TAAAACAGAA
    ATTGACAAGC CGTCCCAGAT GCAGGTGACA GATGTCCAGG ACAACAGCAT CAGTGTCAGG
    TGGCTGCCTT CAACTTCTCC TGTGACAGGC TACAGAGTGA CCACCACTCC CAAAAATGGC
    CTAGGACCAT CAAAAACTAA AACTGCCAGT CCAGATCAAA CAGAAATGAC CATTGAAGGT
    TTGCAACCCA CTGTGGAGTA CGTGGTTAGT GTTTATGCTC AGAACCGGAA CGGAGAAAGC
    CAGCCCCTGG TTCAAACTGC AGTGACCACC ATTCCTGCGC CCACCAATCT GAAGTTCAGT
    CAGGTGACAC CCACCAGCTT TACTGCCCAG TGGATAGCAC CCAGTGTTCA GCTCACTGGC
    TACCGGGTGC GGGTGAACCC GAAAGAGAAG ACAGGACCAA TGAAAGAAAT CAACCTTTCT
    CCAGACAGCT CATCGGTGAT TGTGTCAGGA CTCATGGTGG CCACTAAATA CGAAGTCAGT
    GTCTATGCTC TCAAGGACAC ACTGACAAGC AGACCAGCCC AGGGAGTCAT CACTACTCTG
    GAGAATGTTA GCCCTCCAAG AAGGGCCCGT GTGACGGACG CTACAGAGAC CACCATCACT
    ATTAGCTGGA GAACAAAGAC AGAGACAATC ACTGGCTTCC AAGTCGATGC CATCCCAGCC
    AATGGCCAGA CCCCAGTTCA GAGGAGCATC AGCCCGGATG TTAGAAGCTA CACCATTACA
    GGTTTACAGC CAGGCACTGA CTACAAGATC CACCTGTACA CTCTGAACGA CAATGCCCGG
    AGCTCGCCCG TGATCATCGA TGCCTCCACC GCCATCGACG CACCATCCAA CCTGCGGTTC
    CTGACCACCA CACCCAACTC CTTGCTGGTG TCATGGCAGG CGCCCCGTGC CAGGATTACT
    GGCTACATTA TCAAGTATGA GAAGCCTGGA TCCCCTCCCA GAGAAGTGGT CCCTCGGCCC
    CGCCCTGGTG TCACGGAGGC CACCATTACT GGTCTGGAGC CAGGAACCGA GTACACCATC
    TACGTCATTG CCCTGAAGAA CAATCAGAAG AGTGAGCCCC TGATTGGGAG GAAGAAGACA
    GTTCAAAAGA CCCCCTTCAT CACCAACCCT GGGTATGACA CCGAAAATGG TATTCAGCTT
    CCTGGCACAA CCCACCAGCA ACCCAGTGTT GGGCAACAAA TGATCTTTGA GGAACATGGC
    TTTAGGCGGA CAACGCCACC CACTGCGGCC ACCCCCGTCA GGCTTAGGCC AAGACCATAC
    CTGCCGAATG TAGATGAGGA GGTCCAAATC GGTCATGTTC CCAGGGGAGA CGTAGACTAC
    CACCTCTATC CTCATGTTCC GGGCCTCAAT CCAAATGCCT CTACAGGACA GGAAGCTCTC
    TCTCAGACAA CCATCTCTTG GACGCCGTTC CAGGAGAGTT CTGAGTACAT CATTTCATGC
    CAACCAGTTG GCACCGACGA AGAGCCCTTA CAGTTCCAAG TTCCTGGAAC TTCTACCAGT
    GCGACTCTGA CTGGCCTTAC CAGAGGGGTC ACCTACAACA TCATAGTGGA GGCACTGCAG
    AACCAGAGGA GGCACAAGGT TCGGGAAGAG GTTGTGACTG TGGGCAACGC TGTCAGCGAA
    GGCCTGAACC AGCCTACAGA TGACTCATGC TTTGACCCTT ACACGGTTTC CCATTACGCC
    ATTGGAGAGG AGTGGGAGCG GTTGTCTGAC GCTGGCTTTA AGCTCACATG CCAGTGCTTG
    GGCTTTGGCA GTGGTCATTT CAGATGCGAT TCATCTAAAT GGTGCCATGA CAACGGTGTC
    AACTACAAGA TCGGAGAGAA GTGGGATCGG CAGGGAGAAA ATGGCCAGCG GATGAGCTGC
    ACATGCCTCG GGAATGGAAA GGGAGAATTC AAGTGTGATC CCCATGAAGC AACGTGCTAT
    GACGATGGGA AGACCTACCA TGTAGGAGAA CAGTGGCAGA AAGAATATCT CGGAGCCATT
    TGCTCCTGCA CGTGTTTCGG AGGCCAGCGG GGCTGGCGCT GTGACAACTG CCGTAGACCT
    GGGGCTGCTG AACCCAGTCC CGATGGCACC ACCGGCCACA CCTACAACCA GTATACACAG
    AGATACAATC AGAGAACAAA CACTAACGTA AATTGCCCCA TTGAGTGCTT CATGCCGCTA
    GATGTGCAAG CTGACAGAGA CGATTCTCGA GAGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu23220
    Clone ID Related Accession (Same CDS sequence) NM_001276412.1
    Accession Version NM_001276412.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6801bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-24
    Organism Mus musculus(house mouse)
    Product fibronectin isoform f precursor
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from CJ098561.1, AK147639.1, AK147315.1 and AC124821.5. Transcript Variant: This variant (6) lacks an in-frame exon and uses an alternate in-frame splice site in the coding region, compared to variant 1. The encoded isoform (f) is shorter than isoform a. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK147639.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164134, SAMN01164142 [ECO:0000348] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    ATGCTCAGGG GTCCGGGACC CGGGCGGCTG CTGCTGCTGG CAGTCCTGTG CCTGGGGACC 
    TCGGTGCGCT GCACCGAAGC CGGGAAGAGC AAGAGGCAGG CTCAGCAAAT CGTGCAGCCT
    CAATCCCCGG TGGCTGTCAG TCAGAGCAAG CCTGGCTGTT TTGACAATGG GAAGCACTAT
    CAGATAAATC AGCAGTGGGA ACGGACCTAC CTAGGCAACG CCCTGGTTTG TACCTGCTAT
    GGAGGAAGCC GGGGTTTTAA CTGCGAGAGC AAGCCTGAGC CTGAAGAGAC TTGCTTTGAC
    AAATACACTG GGAACACTTA CAAAGTGGGT GACACTTATG AGCGCCCTAA AGATTCCATG
    ATCTGGGACT GTACCTGCAT CGGGGCTGGG AGAGGCAGGA TCAGCTGTAC CATTGCAAAT
    CGCTGCCATG AAGGGGGTCA GTCCTACAAG ATTGGCGACA AGTGGAGGAG GCCACATGAG
    ACTGGTGGCT ACATGTTAGA GTGTCTGTGT CTGGGAAATG GAAAAGGGGA ATGGACCTGC
    AAACCTATAG CTGAGAAGTG TTTTGATCAT GCTGCTGGGA CGTCCTACGT CGTGGGGGAG
    ACCTGGGAAA AGCCCTACCA AGGCTGGATG ATGGTGGACT GTACTTGTCT AGGCGAAGGC
    AATGGACGCA TCACCTGTAC CTCCAGAAAC AGATGCAACG ATCAGGACAC CCGGACATCC
    TATAGGATTG GAGACACGTG GAGCAAGAAG GACAACCGAG GAAACCTGCT TCAGTGTGTC
    TGCACAGGCA ATGGCAGAGG GGAGTGGAAG TGTGAGCGAC ATGCTCTACA AAGTGCTTCA
    GCCGGATCTG GCTCCTTCAC TGATGTCCGA ACAGCTATTT ACCAACCGCA GACTCACCCC
    CAGCCCGCTC CCTACGGCCA CTGTGTCACC GACAGTGGTG TGGTCTACTC TGTGGGAATG
    CAGTGGCTGA AGTCGCAAGG AAACAAGCAA ATGCTGTGCA CGTGCCTGGG CAATGGCGTC
    AGCTGCCAGG AGACAGCCGT GACCCAGACT TATGGTGGCA ATTCAAACGG GGAGCCCTGT
    GTCCTCCCGT TCACCTACAA CGGTAGGACC TTCTATTCCT GCACCACCGA AGGGCGGCAA
    GACGGACATC TGTGGTGTAG CACAACTTCC AATTACGAAC AAGACCAGAA GTATTCCTTC
    TGCACAGACC ATGCGGTTTT GGTTCAGACT CGAGGCGGAA ATTCCAATGG TGCTCTGTGC
    CACTTCCCCT TCCTGTACAA CAACCGGAAT TACACCGACT GTACTTCTGA GGGTCGCAGG
    GACAACATGA AATGGTGCGG CACCACCCAG AACTACGATG CCGATCAGAA GTTTGGATTC
    TGCCCAATGG CTGCCCACGA GGAGATCTGC ACAACCAATG AAGGGGTCAT GTATCGCATT
    GGGGATCAGT GGGATAAGCA GCATGACCTG GGCCACATGA TGAGGTGCAC GTGTGTGGGG
    AACGGTCGTG GAGAATGGGC CTGCATCCCC TACTCCCAGC TCCGAGACCA GTGCATCGTT
    GATGACATTA CTTACAATGT GAACGACACG TTCCACAAGC GTCACGAGGA GGGACATATG
    CTGAACTGTA CCTGCTTTGG TCAGGGCCGG GGCAGATGGA AGTGTGACCC CATTGACCAG
    TGCCAAGATT CAGAGACCCG GACATTTTAC CAGATTGGTG ACTCCTGGGA GAAGTTTGTG
    CATGGTGTCC GATACCAGTG TTACTGCTAC GGCCGTGGCA TCGGGGAGTG GCACTGTCAA
    CCTCTGCAGA CCTACCCAGG CACAACTGGA CCTGTCCAAG TAATTATCAC GGAGACCCCC
    AGCCAGCCCA ATTCCCACCC CATCCAGTGG AATGCCCCGG AGCCTTCACA CATCACCAAG
    TACATTCTCA GATGGAGACC TAAAACCTCT ACGGGTCGCT GGAAGGAAGC TACCATTCCT
    GGCCACCTTA ACTCCTATAC CATCAAAGGC CTGACCCCAG GTGTGATCTA TGAGGGACAG
    CTCATCAGCA TCCAGCAGTA TGGCCACAGA GAAGTGACAC GCTTCGACTT CACCACCAGC
    GCCAGCACCC CTGTGACCAG CAACACGGTG ACCGGAGAGA CTGCGCCCTA CTCTCCTGTT
    GTGGCCACTT CTGAATCTGT AACTGAAATC ACAGCCAGCA GCTTTGTGGT CTCATGGGTC
    TCAGCCTCCG ACACCGTGTC AGGCTTCCGG GTGGAGTATG AGCTGAGCGA GGAGGGAGAT
    GAACCACAGT ACCTTGATCT CCCAAGCACG GCCACTTCCG TGAACATTCC TGACCTGCTC
    CCGGGCAGAA AGTACATTGT CAATGTCTAT CAGATATCTG AGGAGGGAAA ACAGAGCTTG
    ATCCTGTCTA CCTCACAGAC TACAGCACCT GACGCTCCTC CAGACCCTAC CGTGGACCAG
    GTTGATGATA CTTCCATTGT TGTTCGGTGG AGTAGACCCC AGGCACCTAT CACAGGGTAT
    AGAATTGTCT ATTCACCTTC AGTAGAAGGC AGTAGCACAG AGCTCAACCT CCCTGAAACG
    GCCAACTCCG TCACCCTCAG CGACCTGCAG CCCGGTGTTC AGTACAACAT CACTATCTAT
    GCTGTGGAGG AGAACCAGGA GAGCACACCC GTTTTCATCC AACAAGAGAC CACTGGCACC
    CCAAGATCTG ATAACGTCCC CCCTCCGACG GACCTACAGT TTGTGGAACT GACTGATGTG
    AAAGTCACCA TCATGTGGAC CCCTCCTGAT AGTGTGGTGT CTGGATACCG TGTGGAGGTC
    CTGCCTGTCA GCCTGCCCGG GGAACATGGG CAGAGGCTGC CTGTCAACAG AAATACCTTT
    GCTGAAATCA CTGGGCTGTC CCCTGGGGTC ACGTACCTCT TCAAAGTCTT TGCTGTGCAC
    CAGGGCAGGG AAAGCAATCC TCTGACGGCA CAACAGACCA CCAAACTCGA CGCTCCCACT
    AACCTCCAGT TTGTCAATGA AACTGACAGA ACAGTTCTGG TAACGTGGAC TCCACCTCGA
    GCCCGTATAG CAGGCTACCG ACTGACCGCG GGCCTGACCC GAGGAGGCCA GCCCAAGCAG
    TACAATGTGG GACCCTTGGC CTCCAAGTAT CCCCTGAGAA ATCTGCAGCC TGGGTCTGAG
    TACACCGTGA CCTTGGTGGC TGTGAAAGGG AACCAGCAGA GTCCCAAAGC CACCGGAGTC
    TTTACTACCC TGCAGCCTCT GCGCTCCATT CCACCTTACA ACACCGAGGT GACAGAGACC
    ACAATTGTGA TCACCTGGAC CCCCGCTCCA AGGATTGGCT TCAAGCTGGG TGTACGACCG
    AGCCAGGGAG GTGAGGCACC CCGAGAAGTG ACTTCAGACT CTGGGAGCAT TGTTGTGTCT
    GGCTTGACTC CAGGCGTGGA ATACACTTAC ACCATCCAAG TCCTGCGAGA TGGCCAGGAG
    AGAGATGCAC CGATTGTCAA CAGAGTAGTG ACACCGCTGT CTCCACCGAC CAACTTGCAT
    CTGGAGGCAA ACCCTGACAC TGGAGTGCTT ACTGTCTCCT GGGAGAGGAG CACTACCCCA
    GATATCACTG GCTACAGAAT AACTACTACC CCCACGAACG GGCAGCAGGG GACCTCTCTG
    GAAGAAGTGG TCCATGCTGA TCAGAGTTCC TGCACTTTTG AGAACCTGAA TCCTGGCCTG
    GAGTACAACG TCAGTGTTTA CACTGTCAAA GATGACAAGG AAAGTGCCCC TATCTCTGAT
    ACCGTTGTCC CAGCTGTCCC TCCTCCCACG GATCTGCGAT TCACCAATAT CGGTCCAGAC
    ACGATGCGGG TCACTTGGGC CCCGCCTCCG TCCATCGAGC TGACCAACCT CTTGGTGCGC
    TACTCACCCG TGAAGAATGA AGAGGACGTT GCAGAGCTAT CCATTTCACC TTCAGACAAT
    GCCGTGGTCC TAACAAATCT CCTGCCTGGG ACAGAATACT TAGTCAGTGT CTCCAGTGTC
    TACGAACAAC ATGAGAGCAT CCCTCTCCGG GGAAGGCAGA AAACAGGTCT CGATTCCCCA
    ACTGGTTTTG ATTCTTCTGA TATCACCGCC AACTCATTCA CTGTCCACTG GGTGGCTCCT
    CGGGCCCCCA TCACCGGCTA CATCATCCGC CATCACGCCG AGCATTCTGT CGGAAGACCC
    AGGCAGGATC GAGTGCCGCC CTCGCGGAAT TCCATCACCC TCACCAACCT TAATCCGGGC
    ACCGAGTACG TTGTCAGCAT CATTGCTGTT AATGGCAGAG AGGAGAGCCC GCCACTGATT
    GGCCAGCAAG CCACAGTTTC TGATATTCCG AGAGATCTGG AGGTCATTGC CTCCACCCCC
    ACCAGCCTGC TCATCAGTTG GGAACCCCCT GCCGTCTCTG TGCGCTATTA CAGAATCACC
    TACGGAGAGA CAGGAGGAAA TAGCCCTGTC CAGGAGTTCA CTGTGCCCGG AAGCAAGTCC
    ACAGCCACCA TCAACAACAT TAAACCAGGA GCAGACTACA CCATCACCCT GTATGCTGTC
    ACTGGCCGTG GGGACAGTCC AGCAAGCAGC AAGCCAGTTT CCATCAATTA TAAAACAGAA
    ATTGACAAGC CGTCCCAGAT GCAGGTGACA GATGTCCAGG ACAACAGCAT CAGTGTCAGG
    TGGCTGCCTT CAACTTCTCC TGTGACAGGC TACAGAGTGA CCACCACTCC CAAAAATGGC
    CTAGGACCAT CAAAAACTAA AACTGCCAGT CCAGATCAAA CAGAAATGAC CATTGAAGGT
    TTGCAACCCA CTGTGGAGTA CGTGGTTAGT GTTTATGCTC AGAACCGGAA CGGAGAAAGC
    CAGCCCCTGG TTCAAACTGC AGTGACCAAC ATTGATCGCC CTAAAGGACT GGCATTCACT
    GATGTGGATG TCGATTCCAT CAAAATTGCT TGGGAAAGCC CACAGGGGCA AGTTTCCAGG
    TACAGGGTGA CCTACTCGAG CCCTGAGGAT GGAATCCGGG AGCTTTTCCC TGCACCTGAT
    GGTGAAGACG ACACTGCAGA GCTGCAGGGC CTCAGGCCGG GGTCTGAGTA CACAGTCAGT
    GTGGTTGCCT TGCACGATGA TATGGAGAGC CAGCCCCTGA TTGGAATCCA GTCCACAGCC
    ATTCCTGCGC CCACCAATCT GAAGTTCAGT CAGGTGACAC CCACCAGCTT TACTGCCCAG
    TGGATAGCAC CCAGTGTTCA GCTCACTGGC TACCGGGTGC GGGTGAACCC GAAAGAGAAG
    ACAGGACCAA TGAAAGAAAT CAACCTTTCT CCAGACAGCT CATCGGTGAT TGTGTCAGGA
    CTCATGGTGG CCACTAAATA CGAAGTCAGT GTCTATGCTC TCAAGGACAC ACTGACAAGC
    AGACCAGCCC AGGGAGTCAT CACTACTCTG GAGAATGTTA GCCCTCCAAG AAGGGCCCGT
    GTGACGGACG CTACAGAGAC CACCATCACT ATTAGCTGGA GAACAAAGAC AGAGACAATC
    ACTGGCTTCC AAGTCGATGC CATCCCAGCC AATGGCCAGA CCCCAGTTCA GAGGAGCATC
    AGCCCGGATG TTAGAAGCTA CACCATTACA GGTTTACAGC CAGGCACTGA CTACAAGATC
    CACCTGTACA CTCTGAACGA CAATGCCCGG AGCTCGCCCG TGATCATCGA TGCCTCCACC
    GCCATCGACG CACCATCCAA CCTGCGGTTC CTGACCACCA CACCCAACTC CTTGCTGGTG
    TCATGGCAGG CGCCCCGTGC CAGGATTACT GGCTACATTA TCAAGTATGA GAAGCCTGGA
    TCCCCTCCCA GAGAAGTGGT CCCTCGGCCC CGCCCTGGTG TCACGGAGGC CACCATTACT
    GGTCTGGAGC CAGGAACCGA GTACACCATC TACGTCATTG CCCTGAAGAA CAATCAGAAG
    AGTGAGCCCC TGATTGGGAG GAAGAAGACA GGACAGGAAG CTCTCTCTCA GACAACCATC
    TCTTGGACGC CGTTCCAGGA GAGTTCTGAG TACATCATTT CATGCCAACC AGTTGGCACC
    GACGAAGAGC CCTTACAGTT CCAAGTTCCT GGAACTTCTA CCAGTGCGAC TCTGACTGGC
    CTTACCAGAG GGGTCACCTA CAACATCATA GTGGAGGCAC TGCAGAACCA GAGGAGGCAC
    AAGGTTCGGG AAGAGGTTGT GACTGTGGGC AACGCTGTCA GCGAAGGCCT GAACCAGCCT
    ACAGATGACT CATGCTTTGA CCCTTACACG GTTTCCCATT ACGCCATTGG AGAGGAGTGG
    GAGCGGTTGT CTGACGCTGG CTTTAAGCTC ACATGCCAGT GCTTGGGCTT TGGCAGTGGT
    CATTTCAGAT GCGATTCATC TAAATGGTGC CATGACAACG GTGTCAACTA CAAGATCGGA
    GAGAAGTGGG ATCGGCAGGG AGAAAATGGC CAGCGGATGA GCTGCACATG CCTCGGGAAT
    GGAAAGGGAG AATTCAAGTG TGATCCCCAT GAAGCAACGT GCTATGACGA TGGGAAGACC
    TACCATGTAG GAGAACAGTG GCAGAAAGAA TATCTCGGAG CCATTTGCTC CTGCACGTGT
    TTCGGAGGCC AGCGGGGCTG GCGCTGTGAC AACTGCCGTA GACCTGGGGC TGCTGAACCC
    AGTCCCGATG GCACCACCGG CCACACCTAC AACCAGTATA CACAGAGATA CAATCAGAGA
    ACAAACACTA ACGTAAATTG CCCCATTGAG TGCTTCATGC CGCTAGATGT GCAAGCTGAC
    AGAGACGATT CTCGAGAGTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001263341.1
    CDS253..7053
    Translation

    Target ORF information:

    RefSeq Version NM_001276412.1
    Organism Mus musculus(house mouse)
    Definition Mus musculus fibronectin 1 (Fn1), transcript variant 6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001276412.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    ATGCTCAGGG GTCCGGGACC CGGGCGGCTG CTGCTGCTGG CAGTCCTGTG CCTGGGGACC 
    TCGGTGCGCT GCACCGAAGC CGGGAAGAGC AAGAGGCAGG CTCAGCAAAT CGTGCAGCCT
    CAATCCCCGG TGGCTGTCAG TCAGAGCAAG CCTGGCTGTT TTGACAATGG GAAGCACTAT
    CAGATAAATC AGCAGTGGGA ACGGACCTAC CTAGGCAACG CCCTGGTTTG TACCTGCTAT
    GGAGGAAGCC GGGGTTTTAA CTGCGAGAGC AAGCCTGAGC CTGAAGAGAC TTGCTTTGAC
    AAATACACTG GGAACACTTA CAAAGTGGGT GACACTTATG AGCGCCCTAA AGATTCCATG
    ATCTGGGACT GTACCTGCAT CGGGGCTGGG AGAGGCAGGA TCAGCTGTAC CATTGCAAAT
    CGCTGCCATG AAGGGGGTCA GTCCTACAAG ATTGGCGACA AGTGGAGGAG GCCACATGAG
    ACTGGTGGCT ACATGTTAGA GTGTCTGTGT CTGGGAAATG GAAAAGGGGA ATGGACCTGC
    AAACCTATAG CTGAGAAGTG TTTTGATCAT GCTGCTGGGA CGTCCTACGT CGTGGGGGAG
    ACCTGGGAAA AGCCCTACCA AGGCTGGATG ATGGTGGACT GTACTTGTCT AGGCGAAGGC
    AATGGACGCA TCACCTGTAC CTCCAGAAAC AGATGCAACG ATCAGGACAC CCGGACATCC
    TATAGGATTG GAGACACGTG GAGCAAGAAG GACAACCGAG GAAACCTGCT TCAGTGTGTC
    TGCACAGGCA ATGGCAGAGG GGAGTGGAAG TGTGAGCGAC ATGCTCTACA AAGTGCTTCA
    GCCGGATCTG GCTCCTTCAC TGATGTCCGA ACAGCTATTT ACCAACCGCA GACTCACCCC
    CAGCCCGCTC CCTACGGCCA CTGTGTCACC GACAGTGGTG TGGTCTACTC TGTGGGAATG
    CAGTGGCTGA AGTCGCAAGG AAACAAGCAA ATGCTGTGCA CGTGCCTGGG CAATGGCGTC
    AGCTGCCAGG AGACAGCCGT GACCCAGACT TATGGTGGCA ATTCAAACGG GGAGCCCTGT
    GTCCTCCCGT TCACCTACAA CGGTAGGACC TTCTATTCCT GCACCACCGA AGGGCGGCAA
    GACGGACATC TGTGGTGTAG CACAACTTCC AATTACGAAC AAGACCAGAA GTATTCCTTC
    TGCACAGACC ATGCGGTTTT GGTTCAGACT CGAGGCGGAA ATTCCAATGG TGCTCTGTGC
    CACTTCCCCT TCCTGTACAA CAACCGGAAT TACACCGACT GTACTTCTGA GGGTCGCAGG
    GACAACATGA AATGGTGCGG CACCACCCAG AACTACGATG CCGATCAGAA GTTTGGATTC
    TGCCCAATGG CTGCCCACGA GGAGATCTGC ACAACCAATG AAGGGGTCAT GTATCGCATT
    GGGGATCAGT GGGATAAGCA GCATGACCTG GGCCACATGA TGAGGTGCAC GTGTGTGGGG
    AACGGTCGTG GAGAATGGGC CTGCATCCCC TACTCCCAGC TCCGAGACCA GTGCATCGTT
    GATGACATTA CTTACAATGT GAACGACACG TTCCACAAGC GTCACGAGGA GGGACATATG
    CTGAACTGTA CCTGCTTTGG TCAGGGCCGG GGCAGATGGA AGTGTGACCC CATTGACCAG
    TGCCAAGATT CAGAGACCCG GACATTTTAC CAGATTGGTG ACTCCTGGGA GAAGTTTGTG
    CATGGTGTCC GATACCAGTG TTACTGCTAC GGCCGTGGCA TCGGGGAGTG GCACTGTCAA
    CCTCTGCAGA CCTACCCAGG CACAACTGGA CCTGTCCAAG TAATTATCAC GGAGACCCCC
    AGCCAGCCCA ATTCCCACCC CATCCAGTGG AATGCCCCGG AGCCTTCACA CATCACCAAG
    TACATTCTCA GATGGAGACC TAAAACCTCT ACGGGTCGCT GGAAGGAAGC TACCATTCCT
    GGCCACCTTA ACTCCTATAC CATCAAAGGC CTGACCCCAG GTGTGATCTA TGAGGGACAG
    CTCATCAGCA TCCAGCAGTA TGGCCACAGA GAAGTGACAC GCTTCGACTT CACCACCAGC
    GCCAGCACCC CTGTGACCAG CAACACGGTG ACCGGAGAGA CTGCGCCCTA CTCTCCTGTT
    GTGGCCACTT CTGAATCTGT AACTGAAATC ACAGCCAGCA GCTTTGTGGT CTCATGGGTC
    TCAGCCTCCG ACACCGTGTC AGGCTTCCGG GTGGAGTATG AGCTGAGCGA GGAGGGAGAT
    GAACCACAGT ACCTTGATCT CCCAAGCACG GCCACTTCCG TGAACATTCC TGACCTGCTC
    CCGGGCAGAA AGTACATTGT CAATGTCTAT CAGATATCTG AGGAGGGAAA ACAGAGCTTG
    ATCCTGTCTA CCTCACAGAC TACAGCACCT GACGCTCCTC CAGACCCTAC CGTGGACCAG
    GTTGATGATA CTTCCATTGT TGTTCGGTGG AGTAGACCCC AGGCACCTAT CACAGGGTAT
    AGAATTGTCT ATTCACCTTC AGTAGAAGGC AGTAGCACAG AGCTCAACCT CCCTGAAACG
    GCCAACTCCG TCACCCTCAG CGACCTGCAG CCCGGTGTTC AGTACAACAT CACTATCTAT
    GCTGTGGAGG AGAACCAGGA GAGCACACCC GTTTTCATCC AACAAGAGAC CACTGGCACC
    CCAAGATCTG ATAACGTCCC CCCTCCGACG GACCTACAGT TTGTGGAACT GACTGATGTG
    AAAGTCACCA TCATGTGGAC CCCTCCTGAT AGTGTGGTGT CTGGATACCG TGTGGAGGTC
    CTGCCTGTCA GCCTGCCCGG GGAACATGGG CAGAGGCTGC CTGTCAACAG AAATACCTTT
    GCTGAAATCA CTGGGCTGTC CCCTGGGGTC ACGTACCTCT TCAAAGTCTT TGCTGTGCAC
    CAGGGCAGGG AAAGCAATCC TCTGACGGCA CAACAGACCA CCAAACTCGA CGCTCCCACT
    AACCTCCAGT TTGTCAATGA AACTGACAGA ACAGTTCTGG TAACGTGGAC TCCACCTCGA
    GCCCGTATAG CAGGCTACCG ACTGACCGCG GGCCTGACCC GAGGAGGCCA GCCCAAGCAG
    TACAATGTGG GACCCTTGGC CTCCAAGTAT CCCCTGAGAA ATCTGCAGCC TGGGTCTGAG
    TACACCGTGA CCTTGGTGGC TGTGAAAGGG AACCAGCAGA GTCCCAAAGC CACCGGAGTC
    TTTACTACCC TGCAGCCTCT GCGCTCCATT CCACCTTACA ACACCGAGGT GACAGAGACC
    ACAATTGTGA TCACCTGGAC CCCCGCTCCA AGGATTGGCT TCAAGCTGGG TGTACGACCG
    AGCCAGGGAG GTGAGGCACC CCGAGAAGTG ACTTCAGACT CTGGGAGCAT TGTTGTGTCT
    GGCTTGACTC CAGGCGTGGA ATACACTTAC ACCATCCAAG TCCTGCGAGA TGGCCAGGAG
    AGAGATGCAC CGATTGTCAA CAGAGTAGTG ACACCGCTGT CTCCACCGAC CAACTTGCAT
    CTGGAGGCAA ACCCTGACAC TGGAGTGCTT ACTGTCTCCT GGGAGAGGAG CACTACCCCA
    GATATCACTG GCTACAGAAT AACTACTACC CCCACGAACG GGCAGCAGGG GACCTCTCTG
    GAAGAAGTGG TCCATGCTGA TCAGAGTTCC TGCACTTTTG AGAACCTGAA TCCTGGCCTG
    GAGTACAACG TCAGTGTTTA CACTGTCAAA GATGACAAGG AAAGTGCCCC TATCTCTGAT
    ACCGTTGTCC CAGCTGTCCC TCCTCCCACG GATCTGCGAT TCACCAATAT CGGTCCAGAC
    ACGATGCGGG TCACTTGGGC CCCGCCTCCG TCCATCGAGC TGACCAACCT CTTGGTGCGC
    TACTCACCCG TGAAGAATGA AGAGGACGTT GCAGAGCTAT CCATTTCACC TTCAGACAAT
    GCCGTGGTCC TAACAAATCT CCTGCCTGGG ACAGAATACT TAGTCAGTGT CTCCAGTGTC
    TACGAACAAC ATGAGAGCAT CCCTCTCCGG GGAAGGCAGA AAACAGGTCT CGATTCCCCA
    ACTGGTTTTG ATTCTTCTGA TATCACCGCC AACTCATTCA CTGTCCACTG GGTGGCTCCT
    CGGGCCCCCA TCACCGGCTA CATCATCCGC CATCACGCCG AGCATTCTGT CGGAAGACCC
    AGGCAGGATC GAGTGCCGCC CTCGCGGAAT TCCATCACCC TCACCAACCT TAATCCGGGC
    ACCGAGTACG TTGTCAGCAT CATTGCTGTT AATGGCAGAG AGGAGAGCCC GCCACTGATT
    GGCCAGCAAG CCACAGTTTC TGATATTCCG AGAGATCTGG AGGTCATTGC CTCCACCCCC
    ACCAGCCTGC TCATCAGTTG GGAACCCCCT GCCGTCTCTG TGCGCTATTA CAGAATCACC
    TACGGAGAGA CAGGAGGAAA TAGCCCTGTC CAGGAGTTCA CTGTGCCCGG AAGCAAGTCC
    ACAGCCACCA TCAACAACAT TAAACCAGGA GCAGACTACA CCATCACCCT GTATGCTGTC
    ACTGGCCGTG GGGACAGTCC AGCAAGCAGC AAGCCAGTTT CCATCAATTA TAAAACAGAA
    ATTGACAAGC CGTCCCAGAT GCAGGTGACA GATGTCCAGG ACAACAGCAT CAGTGTCAGG
    TGGCTGCCTT CAACTTCTCC TGTGACAGGC TACAGAGTGA CCACCACTCC CAAAAATGGC
    CTAGGACCAT CAAAAACTAA AACTGCCAGT CCAGATCAAA CAGAAATGAC CATTGAAGGT
    TTGCAACCCA CTGTGGAGTA CGTGGTTAGT GTTTATGCTC AGAACCGGAA CGGAGAAAGC
    CAGCCCCTGG TTCAAACTGC AGTGACCAAC ATTGATCGCC CTAAAGGACT GGCATTCACT
    GATGTGGATG TCGATTCCAT CAAAATTGCT TGGGAAAGCC CACAGGGGCA AGTTTCCAGG
    TACAGGGTGA CCTACTCGAG CCCTGAGGAT GGAATCCGGG AGCTTTTCCC TGCACCTGAT
    GGTGAAGACG ACACTGCAGA GCTGCAGGGC CTCAGGCCGG GGTCTGAGTA CACAGTCAGT
    GTGGTTGCCT TGCACGATGA TATGGAGAGC CAGCCCCTGA TTGGAATCCA GTCCACAGCC
    ATTCCTGCGC CCACCAATCT GAAGTTCAGT CAGGTGACAC CCACCAGCTT TACTGCCCAG
    TGGATAGCAC CCAGTGTTCA GCTCACTGGC TACCGGGTGC GGGTGAACCC GAAAGAGAAG
    ACAGGACCAA TGAAAGAAAT CAACCTTTCT CCAGACAGCT CATCGGTGAT TGTGTCAGGA
    CTCATGGTGG CCACTAAATA CGAAGTCAGT GTCTATGCTC TCAAGGACAC ACTGACAAGC
    AGACCAGCCC AGGGAGTCAT CACTACTCTG GAGAATGTTA GCCCTCCAAG AAGGGCCCGT
    GTGACGGACG CTACAGAGAC CACCATCACT ATTAGCTGGA GAACAAAGAC AGAGACAATC
    ACTGGCTTCC AAGTCGATGC CATCCCAGCC AATGGCCAGA CCCCAGTTCA GAGGAGCATC
    AGCCCGGATG TTAGAAGCTA CACCATTACA GGTTTACAGC CAGGCACTGA CTACAAGATC
    CACCTGTACA CTCTGAACGA CAATGCCCGG AGCTCGCCCG TGATCATCGA TGCCTCCACC
    GCCATCGACG CACCATCCAA CCTGCGGTTC CTGACCACCA CACCCAACTC CTTGCTGGTG
    TCATGGCAGG CGCCCCGTGC CAGGATTACT GGCTACATTA TCAAGTATGA GAAGCCTGGA
    TCCCCTCCCA GAGAAGTGGT CCCTCGGCCC CGCCCTGGTG TCACGGAGGC CACCATTACT
    GGTCTGGAGC CAGGAACCGA GTACACCATC TACGTCATTG CCCTGAAGAA CAATCAGAAG
    AGTGAGCCCC TGATTGGGAG GAAGAAGACA GGACAGGAAG CTCTCTCTCA GACAACCATC
    TCTTGGACGC CGTTCCAGGA GAGTTCTGAG TACATCATTT CATGCCAACC AGTTGGCACC
    GACGAAGAGC CCTTACAGTT CCAAGTTCCT GGAACTTCTA CCAGTGCGAC TCTGACTGGC
    CTTACCAGAG GGGTCACCTA CAACATCATA GTGGAGGCAC TGCAGAACCA GAGGAGGCAC
    AAGGTTCGGG AAGAGGTTGT GACTGTGGGC AACGCTGTCA GCGAAGGCCT GAACCAGCCT
    ACAGATGACT CATGCTTTGA CCCTTACACG GTTTCCCATT ACGCCATTGG AGAGGAGTGG
    GAGCGGTTGT CTGACGCTGG CTTTAAGCTC ACATGCCAGT GCTTGGGCTT TGGCAGTGGT
    CATTTCAGAT GCGATTCATC TAAATGGTGC CATGACAACG GTGTCAACTA CAAGATCGGA
    GAGAAGTGGG ATCGGCAGGG AGAAAATGGC CAGCGGATGA GCTGCACATG CCTCGGGAAT
    GGAAAGGGAG AATTCAAGTG TGATCCCCAT GAAGCAACGT GCTATGACGA TGGGAAGACC
    TACCATGTAG GAGAACAGTG GCAGAAAGAA TATCTCGGAG CCATTTGCTC CTGCACGTGT
    TTCGGAGGCC AGCGGGGCTG GCGCTGTGAC AACTGCCGTA GACCTGGGGC TGCTGAACCC
    AGTCCCGATG GCACCACCGG CCACACCTAC AACCAGTATA CACAGAGATA CAATCAGAGA
    ACAAACACTA ACGTAAATTG CCCCATTGAG TGCTTCATGC CGCTAGATGT GCAAGCTGAC
    AGAGACGATT CTCGAGAGTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu23284
    Clone ID Related Accession (Same CDS sequence) NM_001276413.1
    Accession Version NM_001276413.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6531bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-24
    Organism Mus musculus(house mouse)
    Product fibronectin isoform g precursor
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from CJ098561.1, AK147701.1, AK171339.1 and AC124821.5. Transcript Variant: This variant (7) lacks two in-frame exons and uses an alternate in-frame splice site in the coding region, compared to variant 1. The encoded isoform (g) is shorter than isoform a. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK147701.1, BC145271.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164134, SAMN01164135 [ECO:0000348] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    ATGCTCAGGG GTCCGGGACC CGGGCGGCTG CTGCTGCTGG CAGTCCTGTG CCTGGGGACC 
    TCGGTGCGCT GCACCGAAGC CGGGAAGAGC AAGAGGCAGG CTCAGCAAAT CGTGCAGCCT
    CAATCCCCGG TGGCTGTCAG TCAGAGCAAG CCTGGCTGTT TTGACAATGG GAAGCACTAT
    CAGATAAATC AGCAGTGGGA ACGGACCTAC CTAGGCAACG CCCTGGTTTG TACCTGCTAT
    GGAGGAAGCC GGGGTTTTAA CTGCGAGAGC AAGCCTGAGC CTGAAGAGAC TTGCTTTGAC
    AAATACACTG GGAACACTTA CAAAGTGGGT GACACTTATG AGCGCCCTAA AGATTCCATG
    ATCTGGGACT GTACCTGCAT CGGGGCTGGG AGAGGCAGGA TCAGCTGTAC CATTGCAAAT
    CGCTGCCATG AAGGGGGTCA GTCCTACAAG ATTGGCGACA AGTGGAGGAG GCCACATGAG
    ACTGGTGGCT ACATGTTAGA GTGTCTGTGT CTGGGAAATG GAAAAGGGGA ATGGACCTGC
    AAACCTATAG CTGAGAAGTG TTTTGATCAT GCTGCTGGGA CGTCCTACGT CGTGGGGGAG
    ACCTGGGAAA AGCCCTACCA AGGCTGGATG ATGGTGGACT GTACTTGTCT AGGCGAAGGC
    AATGGACGCA TCACCTGTAC CTCCAGAAAC AGATGCAACG ATCAGGACAC CCGGACATCC
    TATAGGATTG GAGACACGTG GAGCAAGAAG GACAACCGAG GAAACCTGCT TCAGTGTGTC
    TGCACAGGCA ATGGCAGAGG GGAGTGGAAG TGTGAGCGAC ATGCTCTACA AAGTGCTTCA
    GCCGGATCTG GCTCCTTCAC TGATGTCCGA ACAGCTATTT ACCAACCGCA GACTCACCCC
    CAGCCCGCTC CCTACGGCCA CTGTGTCACC GACAGTGGTG TGGTCTACTC TGTGGGAATG
    CAGTGGCTGA AGTCGCAAGG AAACAAGCAA ATGCTGTGCA CGTGCCTGGG CAATGGCGTC
    AGCTGCCAGG AGACAGCCGT GACCCAGACT TATGGTGGCA ATTCAAACGG GGAGCCCTGT
    GTCCTCCCGT TCACCTACAA CGGTAGGACC TTCTATTCCT GCACCACCGA AGGGCGGCAA
    GACGGACATC TGTGGTGTAG CACAACTTCC AATTACGAAC AAGACCAGAA GTATTCCTTC
    TGCACAGACC ATGCGGTTTT GGTTCAGACT CGAGGCGGAA ATTCCAATGG TGCTCTGTGC
    CACTTCCCCT TCCTGTACAA CAACCGGAAT TACACCGACT GTACTTCTGA GGGTCGCAGG
    GACAACATGA AATGGTGCGG CACCACCCAG AACTACGATG CCGATCAGAA GTTTGGATTC
    TGCCCAATGG CTGCCCACGA GGAGATCTGC ACAACCAATG AAGGGGTCAT GTATCGCATT
    GGGGATCAGT GGGATAAGCA GCATGACCTG GGCCACATGA TGAGGTGCAC GTGTGTGGGG
    AACGGTCGTG GAGAATGGGC CTGCATCCCC TACTCCCAGC TCCGAGACCA GTGCATCGTT
    GATGACATTA CTTACAATGT GAACGACACG TTCCACAAGC GTCACGAGGA GGGACATATG
    CTGAACTGTA CCTGCTTTGG TCAGGGCCGG GGCAGATGGA AGTGTGACCC CATTGACCAG
    TGCCAAGATT CAGAGACCCG GACATTTTAC CAGATTGGTG ACTCCTGGGA GAAGTTTGTG
    CATGGTGTCC GATACCAGTG TTACTGCTAC GGCCGTGGCA TCGGGGAGTG GCACTGTCAA
    CCTCTGCAGA CCTACCCAGG CACAACTGGA CCTGTCCAAG TAATTATCAC GGAGACCCCC
    AGCCAGCCCA ATTCCCACCC CATCCAGTGG AATGCCCCGG AGCCTTCACA CATCACCAAG
    TACATTCTCA GATGGAGACC TAAAACCTCT ACGGGTCGCT GGAAGGAAGC TACCATTCCT
    GGCCACCTTA ACTCCTATAC CATCAAAGGC CTGACCCCAG GTGTGATCTA TGAGGGACAG
    CTCATCAGCA TCCAGCAGTA TGGCCACAGA GAAGTGACAC GCTTCGACTT CACCACCAGC
    GCCAGCACCC CTGTGACCAG CAACACGGTG ACCGGAGAGA CTGCGCCCTA CTCTCCTGTT
    GTGGCCACTT CTGAATCTGT AACTGAAATC ACAGCCAGCA GCTTTGTGGT CTCATGGGTC
    TCAGCCTCCG ACACCGTGTC AGGCTTCCGG GTGGAGTATG AGCTGAGCGA GGAGGGAGAT
    GAACCACAGT ACCTTGATCT CCCAAGCACG GCCACTTCCG TGAACATTCC TGACCTGCTC
    CCGGGCAGAA AGTACATTGT CAATGTCTAT CAGATATCTG AGGAGGGAAA ACAGAGCTTG
    ATCCTGTCTA CCTCACAGAC TACAGCACCT GACGCTCCTC CAGACCCTAC CGTGGACCAG
    GTTGATGATA CTTCCATTGT TGTTCGGTGG AGTAGACCCC AGGCACCTAT CACAGGGTAT
    AGAATTGTCT ATTCACCTTC AGTAGAAGGC AGTAGCACAG AGCTCAACCT CCCTGAAACG
    GCCAACTCCG TCACCCTCAG CGACCTGCAG CCCGGTGTTC AGTACAACAT CACTATCTAT
    GCTGTGGAGG AGAACCAGGA GAGCACACCC GTTTTCATCC AACAAGAGAC CACTGGCACC
    CCAAGATCTG ATAACGTCCC CCCTCCGACG GACCTACAGT TTGTGGAACT GACTGATGTG
    AAAGTCACCA TCATGTGGAC CCCTCCTGAT AGTGTGGTGT CTGGATACCG TGTGGAGGTC
    CTGCCTGTCA GCCTGCCCGG GGAACATGGG CAGAGGCTGC CTGTCAACAG AAATACCTTT
    GCTGAAATCA CTGGGCTGTC CCCTGGGGTC ACGTACCTCT TCAAAGTCTT TGCTGTGCAC
    CAGGGCAGGG AAAGCAATCC TCTGACGGCA CAACAGACCA CCAAACTCGA CGCTCCCACT
    AACCTCCAGT TTGTCAATGA AACTGACAGA ACAGTTCTGG TAACGTGGAC TCCACCTCGA
    GCCCGTATAG CAGGCTACCG ACTGACCGCG GGCCTGACCC GAGGAGGCCA GCCCAAGCAG
    TACAATGTGG GACCCTTGGC CTCCAAGTAT CCCCTGAGAA ATCTGCAGCC TGGGTCTGAG
    TACACCGTGA CCTTGGTGGC TGTGAAAGGG AACCAGCAGA GTCCCAAAGC CACCGGAGTC
    TTTACTACCC TGCAGCCTCT GCGCTCCATT CCACCTTACA ACACCGAGGT GACAGAGACC
    ACAATTGTGA TCACCTGGAC CCCCGCTCCA AGGATTGGCT TCAAGCTGGG TGTACGACCG
    AGCCAGGGAG GTGAGGCACC CCGAGAAGTG ACTTCAGACT CTGGGAGCAT TGTTGTGTCT
    GGCTTGACTC CAGGCGTGGA ATACACTTAC ACCATCCAAG TCCTGCGAGA TGGCCAGGAG
    AGAGATGCAC CGATTGTCAA CAGAGTAGTG ACACCGCTGT CTCCACCGAC CAACTTGCAT
    CTGGAGGCAA ACCCTGACAC TGGAGTGCTT ACTGTCTCCT GGGAGAGGAG CACTACCCCA
    GATATCACTG GCTACAGAAT AACTACTACC CCCACGAACG GGCAGCAGGG GACCTCTCTG
    GAAGAAGTGG TCCATGCTGA TCAGAGTTCC TGCACTTTTG AGAACCTGAA TCCTGGCCTG
    GAGTACAACG TCAGTGTTTA CACTGTCAAA GATGACAAGG AAAGTGCCCC TATCTCTGAT
    ACCGTTGTCC CAGCTGTCCC TCCTCCCACG GATCTGCGAT TCACCAATAT CGGTCCAGAC
    ACGATGCGGG TCACTTGGGC CCCGCCTCCG TCCATCGAGC TGACCAACCT CTTGGTGCGC
    TACTCACCCG TGAAGAATGA AGAGGACGTT GCAGAGCTAT CCATTTCACC TTCAGACAAT
    GCCGTGGTCC TAACAAATCT CCTGCCTGGG ACAGAATACT TAGTCAGTGT CTCCAGTGTC
    TACGAACAAC ATGAGAGCAT CCCTCTCCGG GGAAGGCAGA AAACAGGTCT CGATTCCCCA
    ACTGGTTTTG ATTCTTCTGA TATCACCGCC AACTCATTCA CTGTCCACTG GGTGGCTCCT
    CGGGCCCCCA TCACCGGCTA CATCATCCGC CATCACGCCG AGCATTCTGT CGGAAGACCC
    AGGCAGGATC GAGTGCCGCC CTCGCGGAAT TCCATCACCC TCACCAACCT TAATCCGGGC
    ACCGAGTACG TTGTCAGCAT CATTGCTGTT AATGGCAGAG AGGAGAGCCC GCCACTGATT
    GGCCAGCAAG CCACAGTTTC TGATATTCCG AGAGATCTGG AGGTCATTGC CTCCACCCCC
    ACCAGCCTGC TCATCAGTTG GGAACCCCCT GCCGTCTCTG TGCGCTATTA CAGAATCACC
    TACGGAGAGA CAGGAGGAAA TAGCCCTGTC CAGGAGTTCA CTGTGCCCGG AAGCAAGTCC
    ACAGCCACCA TCAACAACAT TAAACCAGGA GCAGACTACA CCATCACCCT GTATGCTGTC
    ACTGGCCGTG GGGACAGTCC AGCAAGCAGC AAGCCAGTTT CCATCAATTA TAAAACAGAA
    ATTGACAAGC CGTCCCAGAT GCAGGTGACA GATGTCCAGG ACAACAGCAT CAGTGTCAGG
    TGGCTGCCTT CAACTTCTCC TGTGACAGGC TACAGAGTGA CCACCACTCC CAAAAATGGC
    CTAGGACCAT CAAAAACTAA AACTGCCAGT CCAGATCAAA CAGAAATGAC CATTGAAGGT
    TTGCAACCCA CTGTGGAGTA CGTGGTTAGT GTTTATGCTC AGAACCGGAA CGGAGAAAGC
    CAGCCCCTGG TTCAAACTGC AGTGACCACC ATTCCTGCGC CCACCAATCT GAAGTTCAGT
    CAGGTGACAC CCACCAGCTT TACTGCCCAG TGGATAGCAC CCAGTGTTCA GCTCACTGGC
    TACCGGGTGC GGGTGAACCC GAAAGAGAAG ACAGGACCAA TGAAAGAAAT CAACCTTTCT
    CCAGACAGCT CATCGGTGAT TGTGTCAGGA CTCATGGTGG CCACTAAATA CGAAGTCAGT
    GTCTATGCTC TCAAGGACAC ACTGACAAGC AGACCAGCCC AGGGAGTCAT CACTACTCTG
    GAGAATGTTA GCCCTCCAAG AAGGGCCCGT GTGACGGACG CTACAGAGAC CACCATCACT
    ATTAGCTGGA GAACAAAGAC AGAGACAATC ACTGGCTTCC AAGTCGATGC CATCCCAGCC
    AATGGCCAGA CCCCAGTTCA GAGGAGCATC AGCCCGGATG TTAGAAGCTA CACCATTACA
    GGTTTACAGC CAGGCACTGA CTACAAGATC CACCTGTACA CTCTGAACGA CAATGCCCGG
    AGCTCGCCCG TGATCATCGA TGCCTCCACC GCCATCGACG CACCATCCAA CCTGCGGTTC
    CTGACCACCA CACCCAACTC CTTGCTGGTG TCATGGCAGG CGCCCCGTGC CAGGATTACT
    GGCTACATTA TCAAGTATGA GAAGCCTGGA TCCCCTCCCA GAGAAGTGGT CCCTCGGCCC
    CGCCCTGGTG TCACGGAGGC CACCATTACT GGTCTGGAGC CAGGAACCGA GTACACCATC
    TACGTCATTG CCCTGAAGAA CAATCAGAAG AGTGAGCCCC TGATTGGGAG GAAGAAGACA
    GGACAGGAAG CTCTCTCTCA GACAACCATC TCTTGGACGC CGTTCCAGGA GAGTTCTGAG
    TACATCATTT CATGCCAACC AGTTGGCACC GACGAAGAGC CCTTACAGTT CCAAGTTCCT
    GGAACTTCTA CCAGTGCGAC TCTGACTGGC CTTACCAGAG GGGTCACCTA CAACATCATA
    GTGGAGGCAC TGCAGAACCA GAGGAGGCAC AAGGTTCGGG AAGAGGTTGT GACTGTGGGC
    AACGCTGTCA GCGAAGGCCT GAACCAGCCT ACAGATGACT CATGCTTTGA CCCTTACACG
    GTTTCCCATT ACGCCATTGG AGAGGAGTGG GAGCGGTTGT CTGACGCTGG CTTTAAGCTC
    ACATGCCAGT GCTTGGGCTT TGGCAGTGGT CATTTCAGAT GCGATTCATC TAAATGGTGC
    CATGACAACG GTGTCAACTA CAAGATCGGA GAGAAGTGGG ATCGGCAGGG AGAAAATGGC
    CAGCGGATGA GCTGCACATG CCTCGGGAAT GGAAAGGGAG AATTCAAGTG TGATCCCCAT
    GAAGCAACGT GCTATGACGA TGGGAAGACC TACCATGTAG GAGAACAGTG GCAGAAAGAA
    TATCTCGGAG CCATTTGCTC CTGCACGTGT TTCGGAGGCC AGCGGGGCTG GCGCTGTGAC
    AACTGCCGTA GACCTGGGGC TGCTGAACCC AGTCCCGATG GCACCACCGG CCACACCTAC
    AACCAGTATA CACAGAGATA CAATCAGAGA ACAAACACTA ACGTAAATTG CCCCATTGAG
    TGCTTCATGC CGCTAGATGT GCAAGCTGAC AGAGACGATT CTCGAGAGTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001263342.1
    CDS253..6783
    Translation

    Target ORF information:

    RefSeq Version NM_001276413.1
    Organism Mus musculus(house mouse)
    Definition Mus musculus fibronectin 1 (Fn1), transcript variant 7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001276413.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    ATGCTCAGGG GTCCGGGACC CGGGCGGCTG CTGCTGCTGG CAGTCCTGTG CCTGGGGACC 
    TCGGTGCGCT GCACCGAAGC CGGGAAGAGC AAGAGGCAGG CTCAGCAAAT CGTGCAGCCT
    CAATCCCCGG TGGCTGTCAG TCAGAGCAAG CCTGGCTGTT TTGACAATGG GAAGCACTAT
    CAGATAAATC AGCAGTGGGA ACGGACCTAC CTAGGCAACG CCCTGGTTTG TACCTGCTAT
    GGAGGAAGCC GGGGTTTTAA CTGCGAGAGC AAGCCTGAGC CTGAAGAGAC TTGCTTTGAC
    AAATACACTG GGAACACTTA CAAAGTGGGT GACACTTATG AGCGCCCTAA AGATTCCATG
    ATCTGGGACT GTACCTGCAT CGGGGCTGGG AGAGGCAGGA TCAGCTGTAC CATTGCAAAT
    CGCTGCCATG AAGGGGGTCA GTCCTACAAG ATTGGCGACA AGTGGAGGAG GCCACATGAG
    ACTGGTGGCT ACATGTTAGA GTGTCTGTGT CTGGGAAATG GAAAAGGGGA ATGGACCTGC
    AAACCTATAG CTGAGAAGTG TTTTGATCAT GCTGCTGGGA CGTCCTACGT CGTGGGGGAG
    ACCTGGGAAA AGCCCTACCA AGGCTGGATG ATGGTGGACT GTACTTGTCT AGGCGAAGGC
    AATGGACGCA TCACCTGTAC CTCCAGAAAC AGATGCAACG ATCAGGACAC CCGGACATCC
    TATAGGATTG GAGACACGTG GAGCAAGAAG GACAACCGAG GAAACCTGCT TCAGTGTGTC
    TGCACAGGCA ATGGCAGAGG GGAGTGGAAG TGTGAGCGAC ATGCTCTACA AAGTGCTTCA
    GCCGGATCTG GCTCCTTCAC TGATGTCCGA ACAGCTATTT ACCAACCGCA GACTCACCCC
    CAGCCCGCTC CCTACGGCCA CTGTGTCACC GACAGTGGTG TGGTCTACTC TGTGGGAATG
    CAGTGGCTGA AGTCGCAAGG AAACAAGCAA ATGCTGTGCA CGTGCCTGGG CAATGGCGTC
    AGCTGCCAGG AGACAGCCGT GACCCAGACT TATGGTGGCA ATTCAAACGG GGAGCCCTGT
    GTCCTCCCGT TCACCTACAA CGGTAGGACC TTCTATTCCT GCACCACCGA AGGGCGGCAA
    GACGGACATC TGTGGTGTAG CACAACTTCC AATTACGAAC AAGACCAGAA GTATTCCTTC
    TGCACAGACC ATGCGGTTTT GGTTCAGACT CGAGGCGGAA ATTCCAATGG TGCTCTGTGC
    CACTTCCCCT TCCTGTACAA CAACCGGAAT TACACCGACT GTACTTCTGA GGGTCGCAGG
    GACAACATGA AATGGTGCGG CACCACCCAG AACTACGATG CCGATCAGAA GTTTGGATTC
    TGCCCAATGG CTGCCCACGA GGAGATCTGC ACAACCAATG AAGGGGTCAT GTATCGCATT
    GGGGATCAGT GGGATAAGCA GCATGACCTG GGCCACATGA TGAGGTGCAC GTGTGTGGGG
    AACGGTCGTG GAGAATGGGC CTGCATCCCC TACTCCCAGC TCCGAGACCA GTGCATCGTT
    GATGACATTA CTTACAATGT GAACGACACG TTCCACAAGC GTCACGAGGA GGGACATATG
    CTGAACTGTA CCTGCTTTGG TCAGGGCCGG GGCAGATGGA AGTGTGACCC CATTGACCAG
    TGCCAAGATT CAGAGACCCG GACATTTTAC CAGATTGGTG ACTCCTGGGA GAAGTTTGTG
    CATGGTGTCC GATACCAGTG TTACTGCTAC GGCCGTGGCA TCGGGGAGTG GCACTGTCAA
    CCTCTGCAGA CCTACCCAGG CACAACTGGA CCTGTCCAAG TAATTATCAC GGAGACCCCC
    AGCCAGCCCA ATTCCCACCC CATCCAGTGG AATGCCCCGG AGCCTTCACA CATCACCAAG
    TACATTCTCA GATGGAGACC TAAAACCTCT ACGGGTCGCT GGAAGGAAGC TACCATTCCT
    GGCCACCTTA ACTCCTATAC CATCAAAGGC CTGACCCCAG GTGTGATCTA TGAGGGACAG
    CTCATCAGCA TCCAGCAGTA TGGCCACAGA GAAGTGACAC GCTTCGACTT CACCACCAGC
    GCCAGCACCC CTGTGACCAG CAACACGGTG ACCGGAGAGA CTGCGCCCTA CTCTCCTGTT
    GTGGCCACTT CTGAATCTGT AACTGAAATC ACAGCCAGCA GCTTTGTGGT CTCATGGGTC
    TCAGCCTCCG ACACCGTGTC AGGCTTCCGG GTGGAGTATG AGCTGAGCGA GGAGGGAGAT
    GAACCACAGT ACCTTGATCT CCCAAGCACG GCCACTTCCG TGAACATTCC TGACCTGCTC
    CCGGGCAGAA AGTACATTGT CAATGTCTAT CAGATATCTG AGGAGGGAAA ACAGAGCTTG
    ATCCTGTCTA CCTCACAGAC TACAGCACCT GACGCTCCTC CAGACCCTAC CGTGGACCAG
    GTTGATGATA CTTCCATTGT TGTTCGGTGG AGTAGACCCC AGGCACCTAT CACAGGGTAT
    AGAATTGTCT ATTCACCTTC AGTAGAAGGC AGTAGCACAG AGCTCAACCT CCCTGAAACG
    GCCAACTCCG TCACCCTCAG CGACCTGCAG CCCGGTGTTC AGTACAACAT CACTATCTAT
    GCTGTGGAGG AGAACCAGGA GAGCACACCC GTTTTCATCC AACAAGAGAC CACTGGCACC
    CCAAGATCTG ATAACGTCCC CCCTCCGACG GACCTACAGT TTGTGGAACT GACTGATGTG
    AAAGTCACCA TCATGTGGAC CCCTCCTGAT AGTGTGGTGT CTGGATACCG TGTGGAGGTC
    CTGCCTGTCA GCCTGCCCGG GGAACATGGG CAGAGGCTGC CTGTCAACAG AAATACCTTT
    GCTGAAATCA CTGGGCTGTC CCCTGGGGTC ACGTACCTCT TCAAAGTCTT TGCTGTGCAC
    CAGGGCAGGG AAAGCAATCC TCTGACGGCA CAACAGACCA CCAAACTCGA CGCTCCCACT
    AACCTCCAGT TTGTCAATGA AACTGACAGA ACAGTTCTGG TAACGTGGAC TCCACCTCGA
    GCCCGTATAG CAGGCTACCG ACTGACCGCG GGCCTGACCC GAGGAGGCCA GCCCAAGCAG
    TACAATGTGG GACCCTTGGC CTCCAAGTAT CCCCTGAGAA ATCTGCAGCC TGGGTCTGAG
    TACACCGTGA CCTTGGTGGC TGTGAAAGGG AACCAGCAGA GTCCCAAAGC CACCGGAGTC
    TTTACTACCC TGCAGCCTCT GCGCTCCATT CCACCTTACA ACACCGAGGT GACAGAGACC
    ACAATTGTGA TCACCTGGAC CCCCGCTCCA AGGATTGGCT TCAAGCTGGG TGTACGACCG
    AGCCAGGGAG GTGAGGCACC CCGAGAAGTG ACTTCAGACT CTGGGAGCAT TGTTGTGTCT
    GGCTTGACTC CAGGCGTGGA ATACACTTAC ACCATCCAAG TCCTGCGAGA TGGCCAGGAG
    AGAGATGCAC CGATTGTCAA CAGAGTAGTG ACACCGCTGT CTCCACCGAC CAACTTGCAT
    CTGGAGGCAA ACCCTGACAC TGGAGTGCTT ACTGTCTCCT GGGAGAGGAG CACTACCCCA
    GATATCACTG GCTACAGAAT AACTACTACC CCCACGAACG GGCAGCAGGG GACCTCTCTG
    GAAGAAGTGG TCCATGCTGA TCAGAGTTCC TGCACTTTTG AGAACCTGAA TCCTGGCCTG
    GAGTACAACG TCAGTGTTTA CACTGTCAAA GATGACAAGG AAAGTGCCCC TATCTCTGAT
    ACCGTTGTCC CAGCTGTCCC TCCTCCCACG GATCTGCGAT TCACCAATAT CGGTCCAGAC
    ACGATGCGGG TCACTTGGGC CCCGCCTCCG TCCATCGAGC TGACCAACCT CTTGGTGCGC
    TACTCACCCG TGAAGAATGA AGAGGACGTT GCAGAGCTAT CCATTTCACC TTCAGACAAT
    GCCGTGGTCC TAACAAATCT CCTGCCTGGG ACAGAATACT TAGTCAGTGT CTCCAGTGTC
    TACGAACAAC ATGAGAGCAT CCCTCTCCGG GGAAGGCAGA AAACAGGTCT CGATTCCCCA
    ACTGGTTTTG ATTCTTCTGA TATCACCGCC AACTCATTCA CTGTCCACTG GGTGGCTCCT
    CGGGCCCCCA TCACCGGCTA CATCATCCGC CATCACGCCG AGCATTCTGT CGGAAGACCC
    AGGCAGGATC GAGTGCCGCC CTCGCGGAAT TCCATCACCC TCACCAACCT TAATCCGGGC
    ACCGAGTACG TTGTCAGCAT CATTGCTGTT AATGGCAGAG AGGAGAGCCC GCCACTGATT
    GGCCAGCAAG CCACAGTTTC TGATATTCCG AGAGATCTGG AGGTCATTGC CTCCACCCCC
    ACCAGCCTGC TCATCAGTTG GGAACCCCCT GCCGTCTCTG TGCGCTATTA CAGAATCACC
    TACGGAGAGA CAGGAGGAAA TAGCCCTGTC CAGGAGTTCA CTGTGCCCGG AAGCAAGTCC
    ACAGCCACCA TCAACAACAT TAAACCAGGA GCAGACTACA CCATCACCCT GTATGCTGTC
    ACTGGCCGTG GGGACAGTCC AGCAAGCAGC AAGCCAGTTT CCATCAATTA TAAAACAGAA
    ATTGACAAGC CGTCCCAGAT GCAGGTGACA GATGTCCAGG ACAACAGCAT CAGTGTCAGG
    TGGCTGCCTT CAACTTCTCC TGTGACAGGC TACAGAGTGA CCACCACTCC CAAAAATGGC
    CTAGGACCAT CAAAAACTAA AACTGCCAGT CCAGATCAAA CAGAAATGAC CATTGAAGGT
    TTGCAACCCA CTGTGGAGTA CGTGGTTAGT GTTTATGCTC AGAACCGGAA CGGAGAAAGC
    CAGCCCCTGG TTCAAACTGC AGTGACCACC ATTCCTGCGC CCACCAATCT GAAGTTCAGT
    CAGGTGACAC CCACCAGCTT TACTGCCCAG TGGATAGCAC CCAGTGTTCA GCTCACTGGC
    TACCGGGTGC GGGTGAACCC GAAAGAGAAG ACAGGACCAA TGAAAGAAAT CAACCTTTCT
    CCAGACAGCT CATCGGTGAT TGTGTCAGGA CTCATGGTGG CCACTAAATA CGAAGTCAGT
    GTCTATGCTC TCAAGGACAC ACTGACAAGC AGACCAGCCC AGGGAGTCAT CACTACTCTG
    GAGAATGTTA GCCCTCCAAG AAGGGCCCGT GTGACGGACG CTACAGAGAC CACCATCACT
    ATTAGCTGGA GAACAAAGAC AGAGACAATC ACTGGCTTCC AAGTCGATGC CATCCCAGCC
    AATGGCCAGA CCCCAGTTCA GAGGAGCATC AGCCCGGATG TTAGAAGCTA CACCATTACA
    GGTTTACAGC CAGGCACTGA CTACAAGATC CACCTGTACA CTCTGAACGA CAATGCCCGG
    AGCTCGCCCG TGATCATCGA TGCCTCCACC GCCATCGACG CACCATCCAA CCTGCGGTTC
    CTGACCACCA CACCCAACTC CTTGCTGGTG TCATGGCAGG CGCCCCGTGC CAGGATTACT
    GGCTACATTA TCAAGTATGA GAAGCCTGGA TCCCCTCCCA GAGAAGTGGT CCCTCGGCCC
    CGCCCTGGTG TCACGGAGGC CACCATTACT GGTCTGGAGC CAGGAACCGA GTACACCATC
    TACGTCATTG CCCTGAAGAA CAATCAGAAG AGTGAGCCCC TGATTGGGAG GAAGAAGACA
    GGACAGGAAG CTCTCTCTCA GACAACCATC TCTTGGACGC CGTTCCAGGA GAGTTCTGAG
    TACATCATTT CATGCCAACC AGTTGGCACC GACGAAGAGC CCTTACAGTT CCAAGTTCCT
    GGAACTTCTA CCAGTGCGAC TCTGACTGGC CTTACCAGAG GGGTCACCTA CAACATCATA
    GTGGAGGCAC TGCAGAACCA GAGGAGGCAC AAGGTTCGGG AAGAGGTTGT GACTGTGGGC
    AACGCTGTCA GCGAAGGCCT GAACCAGCCT ACAGATGACT CATGCTTTGA CCCTTACACG
    GTTTCCCATT ACGCCATTGG AGAGGAGTGG GAGCGGTTGT CTGACGCTGG CTTTAAGCTC
    ACATGCCAGT GCTTGGGCTT TGGCAGTGGT CATTTCAGAT GCGATTCATC TAAATGGTGC
    CATGACAACG GTGTCAACTA CAAGATCGGA GAGAAGTGGG ATCGGCAGGG AGAAAATGGC
    CAGCGGATGA GCTGCACATG CCTCGGGAAT GGAAAGGGAG AATTCAAGTG TGATCCCCAT
    GAAGCAACGT GCTATGACGA TGGGAAGACC TACCATGTAG GAGAACAGTG GCAGAAAGAA
    TATCTCGGAG CCATTTGCTC CTGCACGTGT TTCGGAGGCC AGCGGGGCTG GCGCTGTGAC
    AACTGCCGTA GACCTGGGGC TGCTGAACCC AGTCCCGATG GCACCACCGG CCACACCTAC
    AACCAGTATA CACAGAGATA CAATCAGAGA ACAAACACTA ACGTAAATTG CCCCATTGAG
    TGCTTCATGC CGCTAGATGT GCAAGCTGAC AGAGACGATT CTCGAGAGTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu27265
    Clone ID Related Accession (Same CDS sequence) XM_006495697.3 , XM_006495697.4
    Accession Version XM_006495697.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7359bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-21
    Organism Mus musculus(house mouse)
    Product fibronectin isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006495697.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    ATGCTCAGGG GTCCGGGACC CGGGCGGCTG CTGCTGCTGG CAGTCCTGTG CCTGGGGACC 
    TCGGTGCGCT GCACCGAAGC CGGGAAGAGC AAGAGGCAGG CTCAGCAAAT CGTGCAGCCT
    CAATCCCCGG TGGCTGTCAG TCAGAGCAAG CCTGGCTGTT TTGACAATGG GAAGCACTAT
    CAGATAAATC AGCAGTGGGA ACGGACCTAC CTAGGCAACG CCCTGGTTTG TACCTGCTAT
    GGAGGAAGCC GGGGTTTTAA CTGCGAGAGC AAGCCTGAGC CTGAAGAGAC TTGCTTTGAC
    AAATACACTG GGAACACTTA CAAAGTGGGT GACACTTATG AGCGCCCTAA AGATTCCATG
    ATCTGGGACT GTACCTGCAT CGGGGCTGGG AGAGGCAGGA TCAGCTGTAC CATTGCAAAT
    CGCTGCCATG AAGGGGGTCA GTCCTACAAG ATTGGCGACA AGTGGAGGAG GCCACATGAG
    ACTGGTGGCT ACATGTTAGA GTGTCTGTGT CTGGGAAATG GAAAAGGGGA ATGGACCTGC
    AAACCTATAG CTGAGAAGTG TTTTGATCAT GCTGCTGGGA CGTCCTACGT CGTGGGGGAG
    ACCTGGGAAA AGCCCTACCA AGGCTGGATG ATGGTGGACT GTACTTGTCT AGGCGAAGGC
    AATGGACGCA TCACCTGTAC CTCCAGAAAC AGATGCAACG ATCAGGACAC CCGGACATCC
    TATAGGATTG GAGACACGTG GAGCAAGAAG GACAACCGAG GAAACCTGCT TCAGTGTGTC
    TGCACAGGCA ATGGCAGAGG GGAGTGGAAG TGTGAGCGAC ATGCTCTACA AAGTGCTTCA
    GCCGGATCTG GCTCCTTCAC TGATGTCCGA ACAGCTATTT ACCAACCGCA GACTCACCCC
    CAGCCCGCTC CCTACGGCCA CTGTGTCACC GACAGTGGTG TGGTCTACTC TGTGGGAATG
    CAGTGGCTGA AGTCGCAAGG AAACAAGCAA ATGCTGTGCA CGTGCCTGGG CAATGGCGTC
    AGCTGCCAGG AGACAGCCGT GACCCAGACT TATGGTGGCA ATTCAAACGG GGAGCCCTGT
    GTCCTCCCGT TCACCTACAA CGGTAGGACC TTCTATTCCT GCACCACCGA AGGGCGGCAA
    GACGGACATC TGTGGTGTAG CACAACTTCC AATTACGAAC AAGACCAGAA GTATTCCTTC
    TGCACAGACC ATGCGGTTTT GGTTCAGACT CGAGGCGGAA ATTCCAATGG TGCTCTGTGC
    CACTTCCCCT TCCTGTACAA CAACCGGAAT TACACCGACT GTACTTCTGA GGGTCGCAGG
    GACAACATGA AATGGTGCGG CACCACCCAG AACTACGATG CCGATCAGAA GTTTGGATTC
    TGCCCAATGG CTGCCCACGA GGAGATCTGC ACAACCAATG AAGGGGTCAT GTATCGCATT
    GGGGATCAGT GGGATAAGCA GCATGACCTG GGCCACATGA TGAGGTGCAC GTGTGTGGGG
    AACGGTCGTG GAGAATGGGC CTGCATCCCC TACTCCCAGC TCCGAGACCA GTGCATCGTT
    GATGACATTA CTTACAATGT GAACGACACG TTCCACAAGC GTCACGAGGA GGGACATATG
    CTGAACTGTA CCTGCTTTGG TCAGGGCCGG GGCAGATGGA AGTGTGACCC CATTGACCAG
    TGCCAAGATT CAGAGACCCG GACATTTTAC CAGATTGGTG ACTCCTGGGA GAAGTTTGTG
    CATGGTGTCC GATACCAGTG TTACTGCTAC GGCCGTGGCA TCGGGGAGTG GCACTGTCAA
    CCTCTGCAGA CCTACCCAGG CACAACTGGA CCTGTCCAAG TAATTATCAC GGAGACCCCC
    AGCCAGCCCA ATTCCCACCC CATCCAGTGG AATGCCCCGG AGCCTTCACA CATCACCAAG
    TACATTCTCA GATGGAGACC TAAAACCTCT ACGGGTCGCT GGAAGGAAGC TACCATTCCT
    GGCCACCTTA ACTCCTATAC CATCAAAGGC CTGACCCCAG GTGTGATCTA TGAGGGACAG
    CTCATCAGCA TCCAGCAGTA TGGCCACAGA GAAGTGACAC GCTTCGACTT CACCACCAGC
    GCCAGCACCC CTGTGACCAG CAACACGGTG ACCGGAGAGA CTGCGCCCTA CTCTCCTGTT
    GTGGCCACTT CTGAATCTGT AACTGAAATC ACAGCCAGCA GCTTTGTGGT CTCATGGGTC
    TCAGCCTCCG ACACCGTGTC AGGCTTCCGG GTGGAGTATG AGCTGAGCGA GGAGGGAGAT
    GAACCACAGT ACCTTGATCT CCCAAGCACG GCCACTTCCG TGAACATTCC TGACCTGCTC
    CCGGGCAGAA AGTACATTGT CAATGTCTAT CAGATATCTG AGGAGGGAAA ACAGAGCTTG
    ATCCTGTCTA CCTCACAGAC TACAGCACCT GACGCTCCTC CAGACCCTAC CGTGGACCAG
    GTTGATGATA CTTCCATTGT TGTTCGGTGG AGTAGACCCC AGGCACCTAT CACAGGGTAT
    AGAATTGTCT ATTCACCTTC AGTAGAAGGC AGTAGCACAG AGCTCAACCT CCCTGAAACG
    GCCAACTCCG TCACCCTCAG CGACCTGCAG CCCGGTGTTC AGTACAACAT CACTATCTAT
    GCTGTGGAGG AGAACCAGGA GAGCACACCC GTTTTCATCC AACAAGAGAC CACTGGCACC
    CCAAGATCTG ATAACGTCCC CCCTCCGACG GACCTACAGT TTGTGGAACT GACTGATGTG
    AAAGTCACCA TCATGTGGAC CCCTCCTGAT AGTGTGGTGT CTGGATACCG TGTGGAGGTC
    CTGCCTGTCA GCCTGCCCGG GGAACATGGG CAGAGGCTGC CTGTCAACAG AAATACCTTT
    GCTGAAATCA CTGGGCTGTC CCCTGGGGTC ACGTACCTCT TCAAAGTCTT TGCTGTGCAC
    CAGGGCAGGG AAAGCAATCC TCTGACGGCA CAACAGACCA CCAAACTCGA CGCTCCCACT
    AACCTCCAGT TTGTCAATGA AACTGACAGA ACAGTTCTGG TAACGTGGAC TCCACCTCGA
    GCCCGTATAG CAGGCTACCG ACTGACCGCG GGCCTGACCC GAGGAGGCCA GCCCAAGCAG
    TACAATGTGG GACCCTTGGC CTCCAAGTAT CCCCTGAGAA ATCTGCAGCC TGGGTCTGAG
    TACACCGTGA CCTTGGTGGC TGTGAAAGGG AACCAGCAGA GTCCCAAAGC CACCGGAGTC
    TTTACTACCC TGCAGCCTCT GCGCTCCATT CCACCTTACA ACACCGAGGT GACAGAGACC
    ACAATTGTGA TCACCTGGAC CCCCGCTCCA AGGATTGGCT TCAAGCTGGG TGTACGACCG
    AGCCAGGGAG GTGAGGCACC CCGAGAAGTG ACTTCAGACT CTGGGAGCAT TGTTGTGTCT
    GGCTTGACTC CAGGCGTGGA ATACACTTAC ACCATCCAAG TCCTGCGAGA TGGCCAGGAG
    AGAGATGCAC CGATTGTCAA CAGAGTAGTG ACACCGCTGT CTCCACCGAC CAACTTGCAT
    CTGGAGGCAA ACCCTGACAC TGGAGTGCTT ACTGTCTCCT GGGAGAGGAG CACTACCCCA
    GATATCACTG GCTACAGAAT AACTACTACC CCCACGAACG GGCAGCAGGG GACCTCTCTG
    GAAGAAGTGG TCCATGCTGA TCAGAGTTCC TGCACTTTTG AGAACCTGAA TCCTGGCCTG
    GAGTACAACG TCAGTGTTTA CACTGTCAAA GATGACAAGG AAAGTGCCCC TATCTCTGAT
    ACCGTTGTCC CAGAGGTGCC CCAGCTCACT GACCTAAGCT TTGTTGATAT AACTGATTCA
    AGCATCGGCC TGAGGTGGAC CCCGCTAAAC TCTTCCACCA TTATCGGGTA CCGAATCACA
    GTAGTTGCGG CAGGAGAAGG GATCCCTATT TTTGAAGATT TTGTGGACTC CTCAGTAGGA
    TACTACACAG TTACAGGGCT GGAGCCTGGC ATTGACTATG ACATCAGCGT TATCACTCTC
    ATTAATGGCG GAGAGAGTGC CCCTACTACA CTGACACAGC AAACGGCTGT CCCTCCTCCC
    ACGGATCTGC GATTCACCAA TATCGGTCCA GACACGATGC GGGTCACTTG GGCCCCGCCT
    CCGTCCATCG AGCTGACCAA CCTCTTGGTG CGCTACTCAC CCGTGAAGAA TGAAGAGGAC
    GTTGCAGAGC TATCCATTTC ACCTTCAGAC AATGCCGTGG TCCTAACAAA TCTCCTGCCT
    GGGACAGAAT ACTTAGTCAG TGTCTCCAGT GTCTACGAAC AACATGAGAG CATCCCTCTC
    CGGGGAAGGC AGAAAACAGG TCTCGATTCC CCAACTGGTT TTGATTCTTC TGATATCACC
    GCCAACTCAT TCACTGTCCA CTGGGTGGCT CCTCGGGCCC CCATCACCGG CTACATCATC
    CGCCATCACG CCGAGCATTC TGTCGGAAGA CCCAGGCAGG ATCGAGTGCC GCCCTCGCGG
    AATTCCATCA CCCTCACCAA CCTTAATCCG GGCACCGAGT ACGTTGTCAG CATCATTGCT
    GTTAATGGCA GAGAGGAGAG CCCGCCACTG ATTGGCCAGC AAGCCACAGT TTCTGATATT
    CCGAGAGATC TGGAGGTCAT TGCCTCCACC CCCACCAGCC TGCTCATCAG TTGGGAACCC
    CCTGCCGTCT CTGTGCGCTA TTACAGAATC ACCTACGGAG AGACAGGAGG AAATAGCCCT
    GTCCAGGAGT TCACTGTGCC CGGAAGCAAG TCCACAGCCA CCATCAACAA CATTAAACCA
    GGAGCAGACT ACACCATCAC CCTGTATGCT GTCACTGGCC GTGGGGACAG TCCAGCAAGC
    AGCAAGCCAG TTTCCATCAA TTATAAAACA GAAATTGACA AGCCGTCCCA GATGCAGGTG
    ACAGATGTCC AGGACAACAG CATCAGTGTC AGGTGGCTGC CTTCAACTTC TCCTGTGACA
    GGCTACAGAG TGACCACCAC TCCCAAAAAT GGCCTAGGAC CATCAAAAAC TAAAACTGCC
    AGTCCAGATC AAACAGAAAT GACCATTGAA GGTTTGCAAC CCACTGTGGA GTACGTGGTT
    AGTGTTTATG CTCAGAACCG GAACGGAGAA AGCCAGCCCC TGGTTCAAAC TGCAGTGACC
    AACATTGATC GCCCTAAAGG ACTGGCATTC ACTGATGTGG ATGTCGATTC CATCAAAATT
    GCTTGGGAAA GCCCACAGGG GCAAGTTTCC AGGTACAGGG TGACCTACTC GAGCCCTGAG
    GATGGAATCC GGGAGCTTTT CCCTGCACCT GATGGTGAAG ACGACACTGC AGAGCTGCAG
    GGCCTCAGGC CGGGGTCTGA GTACACAGTC AGTGTGGTTG CCTTGCACGA TGATATGGAG
    AGCCAGCCCC TGATTGGAAT CCAGTCCACA GCCATTCCTG CGCCCACCAA TCTGAAGTTC
    AGTCAGGTGA CACCCACCAG CTTTACTGCC CAGTGGATAG CACCCAGTGT TCAGCTCACT
    GGCTACCGGG TGCGGGTGAA CCCGAAAGAG AAGACAGGAC CAATGAAAGA AATCAACCTT
    TCTCCAGACA GCTCATCGGT GATTGTGTCA GGACTCATGG TGGCCACTAA ATACGAAGTC
    AGTGTCTATG CTCTCAAGGA CACACTGACA AGCAGACCAG CCCAGGGAGT CATCACTACT
    CTGGAGAATG TTAGCCCTCC AAGAAGGGCC CGTGTGACGG ACGCTACAGA GACCACCATC
    ACTATTAGCT GGAGAACAAA GACAGAGACA ATCACTGGCT TCCAAGTCGA TGCCATCCCA
    GCCAATGGCC AGACCCCAGT TCAGAGGAGC ATCAGCCCGG ATGTTAGAAG CTACACCATT
    ACAGGTTTAC AGCCAGGCAC TGACTACAAG ATCCACCTGT ACACTCTGAA CGACAATGCC
    CGGAGCTCGC CCGTGATCAT CGATGCCTCC ACCGCCATCG ACGCACCATC CAACCTGCGG
    TTCCTGACCA CCACACCCAA CTCCTTGCTG GTGTCATGGC AGGCGCCCCG TGCCAGGATT
    ACTGGCTACA TTATCAAGTA TGAGAAGCCT GGATCCCCTC CCAGAGAAGT GGTCCCTCGG
    CCCCGCCCTG GTGTCACGGA GGCCACCATT ACTGGTCTGG AGCCAGGAAC CGAGTACACC
    ATCTACGTCA TTGCCCTGAA GAACAATCAG AAGAGTGAGC CCCTGATTGG GAGGAAGAAG
    ACAGTTCAAA AGACCCCCTT CATCACCAAC CCTGGGTATG ACACCGAAAA TGGTATTCAG
    CTTCCTGGCA CAACCCACCA GCAACCCAGT GTTGGGCAAC AAATGATCTT TGAGGAACAT
    GGCTTTAGGC GGACAACGCC ACCCACTGCG GCCACCCCCG TCAGGCTTAG GCCAAGACCA
    TACCTGCCGA ATGTAGATGA GGAGGTCCAA ATCGGTCATG TTCCCAGGGG AGACGTAGAC
    TACCACCTCT ATCCTCATGT TCCGGGCCTC AATCCAAATG CCTCTACAGG ACAGGAAGCT
    CTCTCTCAGA CAACCATCTC TTGGACGCCG TTCCAGGAGA GTTCTGAGTA CATCATTTCA
    TGCCAACCAG TTGGCACCGA CGAAGAGCCC TTACAGTTCC AAGTTCCTGG AACTTCTACC
    AGTGCGACTC TGACTGGCCT TACCAGAGGG GTCACCTACA ACATCATAGT GGAGGCACTG
    CAGAACCAGA GGAGGCACAA GGTTCGGGAA GAGGTTGTGA CTGTGGGCAA CGCTGTCAGC
    GAAGGCCTGA ACCAGCCTAC AGATGACTCA TGCTTTGACC CTTACACGGT TTCCCATTAC
    GCCATTGGAG AGGAGTGGGA GCGGTTGTCT GACGCTGGCT TTAAGCTCAC ATGCCAGTGC
    TTGGGCTTTG GCAGTGGTCA TTTCAGATGC GATTCATCTA AATGGTGCCA TGACAACGGT
    GTCAACTACA AGATCGGAGA GAAGTGGGAT CGGCAGGGAG AAAATGGCCA GCGGATGAGC
    TGCACATGCC TCGGGAATGG AAAGGGAGAA TTCAAGTGTG ATCCCCATGA AGCAACGTGC
    TATGACGATG GGAAGACCTA CCATGTAGGA GAACAGTGGC AGAAAGAATA TCTCGGAGCC
    ATTTGCTCCT GCACGTGTTT CGGAGGCCAG CGGGGCTGGC GCTGTGACAA CTGCCGTAGA
    CCTGGGGCTG CTGAACCCAG TCCCGATGGC ACCACCGGCC ACACCTACAA CCAGTATACA
    CAGAGATACA ATCAGAGAAC AAACACTAAC GTAAATTGCC CCATTGAGTG CTTCATGCCG
    CTAGATGTGC AAGCTGACAG AGACGATTCT CGAGAGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006495760.1
    CDS248..7606
    Translation

    Target ORF information:

    RefSeq Version XM_006495697.3
    Organism Mus musculus(house mouse)
    Definition Mus musculus fibronectin 1 (Fn1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006495697.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    ATGCTCAGGG GTCCGGGACC CGGGCGGCTG CTGCTGCTGG CAGTCCTGTG CCTGGGGACC 
    TCGGTGCGCT GCACCGAAGC CGGGAAGAGC AAGAGGCAGG CTCAGCAAAT CGTGCAGCCT
    CAATCCCCGG TGGCTGTCAG TCAGAGCAAG CCTGGCTGTT TTGACAATGG GAAGCACTAT
    CAGATAAATC AGCAGTGGGA ACGGACCTAC CTAGGCAACG CCCTGGTTTG TACCTGCTAT
    GGAGGAAGCC GGGGTTTTAA CTGCGAGAGC AAGCCTGAGC CTGAAGAGAC TTGCTTTGAC
    AAATACACTG GGAACACTTA CAAAGTGGGT GACACTTATG AGCGCCCTAA AGATTCCATG
    ATCTGGGACT GTACCTGCAT CGGGGCTGGG AGAGGCAGGA TCAGCTGTAC CATTGCAAAT
    CGCTGCCATG AAGGGGGTCA GTCCTACAAG ATTGGCGACA AGTGGAGGAG GCCACATGAG
    ACTGGTGGCT ACATGTTAGA GTGTCTGTGT CTGGGAAATG GAAAAGGGGA ATGGACCTGC
    AAACCTATAG CTGAGAAGTG TTTTGATCAT GCTGCTGGGA CGTCCTACGT CGTGGGGGAG
    ACCTGGGAAA AGCCCTACCA AGGCTGGATG ATGGTGGACT GTACTTGTCT AGGCGAAGGC
    AATGGACGCA TCACCTGTAC CTCCAGAAAC AGATGCAACG ATCAGGACAC CCGGACATCC
    TATAGGATTG GAGACACGTG GAGCAAGAAG GACAACCGAG GAAACCTGCT TCAGTGTGTC
    TGCACAGGCA ATGGCAGAGG GGAGTGGAAG TGTGAGCGAC ATGCTCTACA AAGTGCTTCA
    GCCGGATCTG GCTCCTTCAC TGATGTCCGA ACAGCTATTT ACCAACCGCA GACTCACCCC
    CAGCCCGCTC CCTACGGCCA CTGTGTCACC GACAGTGGTG TGGTCTACTC TGTGGGAATG
    CAGTGGCTGA AGTCGCAAGG AAACAAGCAA ATGCTGTGCA CGTGCCTGGG CAATGGCGTC
    AGCTGCCAGG AGACAGCCGT GACCCAGACT TATGGTGGCA ATTCAAACGG GGAGCCCTGT
    GTCCTCCCGT TCACCTACAA CGGTAGGACC TTCTATTCCT GCACCACCGA AGGGCGGCAA
    GACGGACATC TGTGGTGTAG CACAACTTCC AATTACGAAC AAGACCAGAA GTATTCCTTC
    TGCACAGACC ATGCGGTTTT GGTTCAGACT CGAGGCGGAA ATTCCAATGG TGCTCTGTGC
    CACTTCCCCT TCCTGTACAA CAACCGGAAT TACACCGACT GTACTTCTGA GGGTCGCAGG
    GACAACATGA AATGGTGCGG CACCACCCAG AACTACGATG CCGATCAGAA GTTTGGATTC
    TGCCCAATGG CTGCCCACGA GGAGATCTGC ACAACCAATG AAGGGGTCAT GTATCGCATT
    GGGGATCAGT GGGATAAGCA GCATGACCTG GGCCACATGA TGAGGTGCAC GTGTGTGGGG
    AACGGTCGTG GAGAATGGGC CTGCATCCCC TACTCCCAGC TCCGAGACCA GTGCATCGTT
    GATGACATTA CTTACAATGT GAACGACACG TTCCACAAGC GTCACGAGGA GGGACATATG
    CTGAACTGTA CCTGCTTTGG TCAGGGCCGG GGCAGATGGA AGTGTGACCC CATTGACCAG
    TGCCAAGATT CAGAGACCCG GACATTTTAC CAGATTGGTG ACTCCTGGGA GAAGTTTGTG
    CATGGTGTCC GATACCAGTG TTACTGCTAC GGCCGTGGCA TCGGGGAGTG GCACTGTCAA
    CCTCTGCAGA CCTACCCAGG CACAACTGGA CCTGTCCAAG TAATTATCAC GGAGACCCCC
    AGCCAGCCCA ATTCCCACCC CATCCAGTGG AATGCCCCGG AGCCTTCACA CATCACCAAG
    TACATTCTCA GATGGAGACC TAAAACCTCT ACGGGTCGCT GGAAGGAAGC TACCATTCCT
    GGCCACCTTA ACTCCTATAC CATCAAAGGC CTGACCCCAG GTGTGATCTA TGAGGGACAG
    CTCATCAGCA TCCAGCAGTA TGGCCACAGA GAAGTGACAC GCTTCGACTT CACCACCAGC
    GCCAGCACCC CTGTGACCAG CAACACGGTG ACCGGAGAGA CTGCGCCCTA CTCTCCTGTT
    GTGGCCACTT CTGAATCTGT AACTGAAATC ACAGCCAGCA GCTTTGTGGT CTCATGGGTC
    TCAGCCTCCG ACACCGTGTC AGGCTTCCGG GTGGAGTATG AGCTGAGCGA GGAGGGAGAT
    GAACCACAGT ACCTTGATCT CCCAAGCACG GCCACTTCCG TGAACATTCC TGACCTGCTC
    CCGGGCAGAA AGTACATTGT CAATGTCTAT CAGATATCTG AGGAGGGAAA ACAGAGCTTG
    ATCCTGTCTA CCTCACAGAC TACAGCACCT GACGCTCCTC CAGACCCTAC CGTGGACCAG
    GTTGATGATA CTTCCATTGT TGTTCGGTGG AGTAGACCCC AGGCACCTAT CACAGGGTAT
    AGAATTGTCT ATTCACCTTC AGTAGAAGGC AGTAGCACAG AGCTCAACCT CCCTGAAACG
    GCCAACTCCG TCACCCTCAG CGACCTGCAG CCCGGTGTTC AGTACAACAT CACTATCTAT
    GCTGTGGAGG AGAACCAGGA GAGCACACCC GTTTTCATCC AACAAGAGAC CACTGGCACC
    CCAAGATCTG ATAACGTCCC CCCTCCGACG GACCTACAGT TTGTGGAACT GACTGATGTG
    AAAGTCACCA TCATGTGGAC CCCTCCTGAT AGTGTGGTGT CTGGATACCG TGTGGAGGTC
    CTGCCTGTCA GCCTGCCCGG GGAACATGGG CAGAGGCTGC CTGTCAACAG AAATACCTTT
    GCTGAAATCA CTGGGCTGTC CCCTGGGGTC ACGTACCTCT TCAAAGTCTT TGCTGTGCAC
    CAGGGCAGGG AAAGCAATCC TCTGACGGCA CAACAGACCA CCAAACTCGA CGCTCCCACT
    AACCTCCAGT TTGTCAATGA AACTGACAGA ACAGTTCTGG TAACGTGGAC TCCACCTCGA
    GCCCGTATAG CAGGCTACCG ACTGACCGCG GGCCTGACCC GAGGAGGCCA GCCCAAGCAG
    TACAATGTGG GACCCTTGGC CTCCAAGTAT CCCCTGAGAA ATCTGCAGCC TGGGTCTGAG
    TACACCGTGA CCTTGGTGGC TGTGAAAGGG AACCAGCAGA GTCCCAAAGC CACCGGAGTC
    TTTACTACCC TGCAGCCTCT GCGCTCCATT CCACCTTACA ACACCGAGGT GACAGAGACC
    ACAATTGTGA TCACCTGGAC CCCCGCTCCA AGGATTGGCT TCAAGCTGGG TGTACGACCG
    AGCCAGGGAG GTGAGGCACC CCGAGAAGTG ACTTCAGACT CTGGGAGCAT TGTTGTGTCT
    GGCTTGACTC CAGGCGTGGA ATACACTTAC ACCATCCAAG TCCTGCGAGA TGGCCAGGAG
    AGAGATGCAC CGATTGTCAA CAGAGTAGTG ACACCGCTGT CTCCACCGAC CAACTTGCAT
    CTGGAGGCAA ACCCTGACAC TGGAGTGCTT ACTGTCTCCT GGGAGAGGAG CACTACCCCA
    GATATCACTG GCTACAGAAT AACTACTACC CCCACGAACG GGCAGCAGGG GACCTCTCTG
    GAAGAAGTGG TCCATGCTGA TCAGAGTTCC TGCACTTTTG AGAACCTGAA TCCTGGCCTG
    GAGTACAACG TCAGTGTTTA CACTGTCAAA GATGACAAGG AAAGTGCCCC TATCTCTGAT
    ACCGTTGTCC CAGAGGTGCC CCAGCTCACT GACCTAAGCT TTGTTGATAT AACTGATTCA
    AGCATCGGCC TGAGGTGGAC CCCGCTAAAC TCTTCCACCA TTATCGGGTA CCGAATCACA
    GTAGTTGCGG CAGGAGAAGG GATCCCTATT TTTGAAGATT TTGTGGACTC CTCAGTAGGA
    TACTACACAG TTACAGGGCT GGAGCCTGGC ATTGACTATG ACATCAGCGT TATCACTCTC
    ATTAATGGCG GAGAGAGTGC CCCTACTACA CTGACACAGC AAACGGCTGT CCCTCCTCCC
    ACGGATCTGC GATTCACCAA TATCGGTCCA GACACGATGC GGGTCACTTG GGCCCCGCCT
    CCGTCCATCG AGCTGACCAA CCTCTTGGTG CGCTACTCAC CCGTGAAGAA TGAAGAGGAC
    GTTGCAGAGC TATCCATTTC ACCTTCAGAC AATGCCGTGG TCCTAACAAA TCTCCTGCCT
    GGGACAGAAT ACTTAGTCAG TGTCTCCAGT GTCTACGAAC AACATGAGAG CATCCCTCTC
    CGGGGAAGGC AGAAAACAGG TCTCGATTCC CCAACTGGTT TTGATTCTTC TGATATCACC
    GCCAACTCAT TCACTGTCCA CTGGGTGGCT CCTCGGGCCC CCATCACCGG CTACATCATC
    CGCCATCACG CCGAGCATTC TGTCGGAAGA CCCAGGCAGG ATCGAGTGCC GCCCTCGCGG
    AATTCCATCA CCCTCACCAA CCTTAATCCG GGCACCGAGT ACGTTGTCAG CATCATTGCT
    GTTAATGGCA GAGAGGAGAG CCCGCCACTG ATTGGCCAGC AAGCCACAGT TTCTGATATT
    CCGAGAGATC TGGAGGTCAT TGCCTCCACC CCCACCAGCC TGCTCATCAG TTGGGAACCC
    CCTGCCGTCT CTGTGCGCTA TTACAGAATC ACCTACGGAG AGACAGGAGG AAATAGCCCT
    GTCCAGGAGT TCACTGTGCC CGGAAGCAAG TCCACAGCCA CCATCAACAA CATTAAACCA
    GGAGCAGACT ACACCATCAC CCTGTATGCT GTCACTGGCC GTGGGGACAG TCCAGCAAGC
    AGCAAGCCAG TTTCCATCAA TTATAAAACA GAAATTGACA AGCCGTCCCA GATGCAGGTG
    ACAGATGTCC AGGACAACAG CATCAGTGTC AGGTGGCTGC CTTCAACTTC TCCTGTGACA
    GGCTACAGAG TGACCACCAC TCCCAAAAAT GGCCTAGGAC CATCAAAAAC TAAAACTGCC
    AGTCCAGATC AAACAGAAAT GACCATTGAA GGTTTGCAAC CCACTGTGGA GTACGTGGTT
    AGTGTTTATG CTCAGAACCG GAACGGAGAA AGCCAGCCCC TGGTTCAAAC TGCAGTGACC
    AACATTGATC GCCCTAAAGG ACTGGCATTC ACTGATGTGG ATGTCGATTC CATCAAAATT
    GCTTGGGAAA GCCCACAGGG GCAAGTTTCC AGGTACAGGG TGACCTACTC GAGCCCTGAG
    GATGGAATCC GGGAGCTTTT CCCTGCACCT GATGGTGAAG ACGACACTGC AGAGCTGCAG
    GGCCTCAGGC CGGGGTCTGA GTACACAGTC AGTGTGGTTG CCTTGCACGA TGATATGGAG
    AGCCAGCCCC TGATTGGAAT CCAGTCCACA GCCATTCCTG CGCCCACCAA TCTGAAGTTC
    AGTCAGGTGA CACCCACCAG CTTTACTGCC CAGTGGATAG CACCCAGTGT TCAGCTCACT
    GGCTACCGGG TGCGGGTGAA CCCGAAAGAG AAGACAGGAC CAATGAAAGA AATCAACCTT
    TCTCCAGACA GCTCATCGGT GATTGTGTCA GGACTCATGG TGGCCACTAA ATACGAAGTC
    AGTGTCTATG CTCTCAAGGA CACACTGACA AGCAGACCAG CCCAGGGAGT CATCACTACT
    CTGGAGAATG TTAGCCCTCC AAGAAGGGCC CGTGTGACGG ACGCTACAGA GACCACCATC
    ACTATTAGCT GGAGAACAAA GACAGAGACA ATCACTGGCT TCCAAGTCGA TGCCATCCCA
    GCCAATGGCC AGACCCCAGT TCAGAGGAGC ATCAGCCCGG ATGTTAGAAG CTACACCATT
    ACAGGTTTAC AGCCAGGCAC TGACTACAAG ATCCACCTGT ACACTCTGAA CGACAATGCC
    CGGAGCTCGC CCGTGATCAT CGATGCCTCC ACCGCCATCG ACGCACCATC CAACCTGCGG
    TTCCTGACCA CCACACCCAA CTCCTTGCTG GTGTCATGGC AGGCGCCCCG TGCCAGGATT
    ACTGGCTACA TTATCAAGTA TGAGAAGCCT GGATCCCCTC CCAGAGAAGT GGTCCCTCGG
    CCCCGCCCTG GTGTCACGGA GGCCACCATT ACTGGTCTGG AGCCAGGAAC CGAGTACACC
    ATCTACGTCA TTGCCCTGAA GAACAATCAG AAGAGTGAGC CCCTGATTGG GAGGAAGAAG
    ACAGTTCAAA AGACCCCCTT CATCACCAAC CCTGGGTATG ACACCGAAAA TGGTATTCAG
    CTTCCTGGCA CAACCCACCA GCAACCCAGT GTTGGGCAAC AAATGATCTT TGAGGAACAT
    GGCTTTAGGC GGACAACGCC ACCCACTGCG GCCACCCCCG TCAGGCTTAG GCCAAGACCA
    TACCTGCCGA ATGTAGATGA GGAGGTCCAA ATCGGTCATG TTCCCAGGGG AGACGTAGAC
    TACCACCTCT ATCCTCATGT TCCGGGCCTC AATCCAAATG CCTCTACAGG ACAGGAAGCT
    CTCTCTCAGA CAACCATCTC TTGGACGCCG TTCCAGGAGA GTTCTGAGTA CATCATTTCA
    TGCCAACCAG TTGGCACCGA CGAAGAGCCC TTACAGTTCC AAGTTCCTGG AACTTCTACC
    AGTGCGACTC TGACTGGCCT TACCAGAGGG GTCACCTACA ACATCATAGT GGAGGCACTG
    CAGAACCAGA GGAGGCACAA GGTTCGGGAA GAGGTTGTGA CTGTGGGCAA CGCTGTCAGC
    GAAGGCCTGA ACCAGCCTAC AGATGACTCA TGCTTTGACC CTTACACGGT TTCCCATTAC
    GCCATTGGAG AGGAGTGGGA GCGGTTGTCT GACGCTGGCT TTAAGCTCAC ATGCCAGTGC
    TTGGGCTTTG GCAGTGGTCA TTTCAGATGC GATTCATCTA AATGGTGCCA TGACAACGGT
    GTCAACTACA AGATCGGAGA GAAGTGGGAT CGGCAGGGAG AAAATGGCCA GCGGATGAGC
    TGCACATGCC TCGGGAATGG AAAGGGAGAA TTCAAGTGTG ATCCCCATGA AGCAACGTGC
    TATGACGATG GGAAGACCTA CCATGTAGGA GAACAGTGGC AGAAAGAATA TCTCGGAGCC
    ATTTGCTCCT GCACGTGTTT CGGAGGCCAG CGGGGCTGGC GCTGTGACAA CTGCCGTAGA
    CCTGGGGCTG CTGAACCCAG TCCCGATGGC ACCACCGGCC ACACCTACAA CCAGTATACA
    CAGAGATACA ATCAGAGAAC AAACACTAAC GTAAATTGCC CCATTGAGTG CTTCATGCCG
    CTAGATGTGC AAGCTGACAG AGACGATTCT CGAGAGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu27267
    Clone ID Related Accession (Same CDS sequence) XM_006495699.3 , XM_006495699.4
    Accession Version XM_006495699.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7164bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-21
    Organism Mus musculus(house mouse)
    Product fibronectin isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006495699.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    ATGCTCAGGG GTCCGGGACC CGGGCGGCTG CTGCTGCTGG CAGTCCTGTG CCTGGGGACC 
    TCGGTGCGCT GCACCGAAGC CGGGAAGAGC AAGAGGCAGG CTCAGCAAAT CGTGCAGCCT
    CAATCCCCGG TGGCTGTCAG TCAGAGCAAG CCTGGCTGTT TTGACAATGG GAAGCACTAT
    CAGATAAATC AGCAGTGGGA ACGGACCTAC CTAGGCAACG CCCTGGTTTG TACCTGCTAT
    GGAGGAAGCC GGGGTTTTAA CTGCGAGAGC AAGCCTGAGC CTGAAGAGAC TTGCTTTGAC
    AAATACACTG GGAACACTTA CAAAGTGGGT GACACTTATG AGCGCCCTAA AGATTCCATG
    ATCTGGGACT GTACCTGCAT CGGGGCTGGG AGAGGCAGGA TCAGCTGTAC CATTGCAAAT
    CGCTGCCATG AAGGGGGTCA GTCCTACAAG ATTGGCGACA AGTGGAGGAG GCCACATGAG
    ACTGGTGGCT ACATGTTAGA GTGTCTGTGT CTGGGAAATG GAAAAGGGGA ATGGACCTGC
    AAACCTATAG CTGAGAAGTG TTTTGATCAT GCTGCTGGGA CGTCCTACGT CGTGGGGGAG
    ACCTGGGAAA AGCCCTACCA AGGCTGGATG ATGGTGGACT GTACTTGTCT AGGCGAAGGC
    AATGGACGCA TCACCTGTAC CTCCAGAAAC AGATGCAACG ATCAGGACAC CCGGACATCC
    TATAGGATTG GAGACACGTG GAGCAAGAAG GACAACCGAG GAAACCTGCT TCAGTGTGTC
    TGCACAGGCA ATGGCAGAGG GGAGTGGAAG TGTGAGCGAC ATGCTCTACA AAGTGCTTCA
    GCCGGATCTG GCTCCTTCAC TGATGTCCGA ACAGCTATTT ACCAACCGCA GACTCACCCC
    CAGCCCGCTC CCTACGGCCA CTGTGTCACC GACAGTGGTG TGGTCTACTC TGTGGGAATG
    CAGTGGCTGA AGTCGCAAGG AAACAAGCAA ATGCTGTGCA CGTGCCTGGG CAATGGCGTC
    AGCTGCCAGG AGACAGCCGT GACCCAGACT TATGGTGGCA ATTCAAACGG GGAGCCCTGT
    GTCCTCCCGT TCACCTACAA CGGTAGGACC TTCTATTCCT GCACCACCGA AGGGCGGCAA
    GACGGACATC TGTGGTGTAG CACAACTTCC AATTACGAAC AAGACCAGAA GTATTCCTTC
    TGCACAGACC ATGCGGTTTT GGTTCAGACT CGAGGCGGAA ATTCCAATGG TGCTCTGTGC
    CACTTCCCCT TCCTGTACAA CAACCGGAAT TACACCGACT GTACTTCTGA GGGTCGCAGG
    GACAACATGA AATGGTGCGG CACCACCCAG AACTACGATG CCGATCAGAA GTTTGGATTC
    TGCCCAATGG CTGCCCACGA GGAGATCTGC ACAACCAATG AAGGGGTCAT GTATCGCATT
    GGGGATCAGT GGGATAAGCA GCATGACCTG GGCCACATGA TGAGGTGCAC GTGTGTGGGG
    AACGGTCGTG GAGAATGGGC CTGCATCCCC TACTCCCAGC TCCGAGACCA GTGCATCGTT
    GATGACATTA CTTACAATGT GAACGACACG TTCCACAAGC GTCACGAGGA GGGACATATG
    CTGAACTGTA CCTGCTTTGG TCAGGGCCGG GGCAGATGGA AGTGTGACCC CATTGACCAG
    TGCCAAGATT CAGAGACCCG GACATTTTAC CAGATTGGTG ACTCCTGGGA GAAGTTTGTG
    CATGGTGTCC GATACCAGTG TTACTGCTAC GGCCGTGGCA TCGGGGAGTG GCACTGTCAA
    CCTCTGCAGA CCTACCCAGG CACAACTGGA CCTGTCCAAG TAATTATCAC GGAGACCCCC
    AGCCAGCCCA ATTCCCACCC CATCCAGTGG AATGCCCCGG AGCCTTCACA CATCACCAAG
    TACATTCTCA GATGGAGACC TAAAACCTCT ACGGGTCGCT GGAAGGAAGC TACCATTCCT
    GGCCACCTTA ACTCCTATAC CATCAAAGGC CTGACCCCAG GTGTGATCTA TGAGGGACAG
    CTCATCAGCA TCCAGCAGTA TGGCCACAGA GAAGTGACAC GCTTCGACTT CACCACCAGC
    GCCAGCACCC CTGTGACCAG CAACACGGTG ACCGGAGAGA CTGCGCCCTA CTCTCCTGTT
    GTGGCCACTT CTGAATCTGT AACTGAAATC ACAGCCAGCA GCTTTGTGGT CTCATGGGTC
    TCAGCCTCCG ACACCGTGTC AGGCTTCCGG GTGGAGTATG AGCTGAGCGA GGAGGGAGAT
    GAACCACAGT ACCTTGATCT CCCAAGCACG GCCACTTCCG TGAACATTCC TGACCTGCTC
    CCGGGCAGAA AGTACATTGT CAATGTCTAT CAGATATCTG AGGAGGGAAA ACAGAGCTTG
    ATCCTGTCTA CCTCACAGAC TACAGCACCT GACGCTCCTC CAGACCCTAC CGTGGACCAG
    GTTGATGATA CTTCCATTGT TGTTCGGTGG AGTAGACCCC AGGCACCTAT CACAGGGTAT
    AGAATTGTCT ATTCACCTTC AGTAGAAGGC AGTAGCACAG AGCTCAACCT CCCTGAAACG
    GCCAACTCCG TCACCCTCAG CGACCTGCAG CCCGGTGTTC AGTACAACAT CACTATCTAT
    GCTGTGGAGG AGAACCAGGA GAGCACACCC GTTTTCATCC AACAAGAGAC CACTGGCACC
    CCAAGATCTG ATAACGTCCC CCCTCCGACG GACCTACAGT TTGTGGAACT GACTGATGTG
    AAAGTCACCA TCATGTGGAC CCCTCCTGAT AGTGTGGTGT CTGGATACCG TGTGGAGGTC
    CTGCCTGTCA GCCTGCCCGG GGAACATGGG CAGAGGCTGC CTGTCAACAG AAATACCTTT
    GCTGAAATCA CTGGGCTGTC CCCTGGGGTC ACGTACCTCT TCAAAGTCTT TGCTGTGCAC
    CAGGGCAGGG AAAGCAATCC TCTGACGGCA CAACAGACCA CCAAACTCGA CGCTCCCACT
    AACCTCCAGT TTGTCAATGA AACTGACAGA ACAGTTCTGG TAACGTGGAC TCCACCTCGA
    GCCCGTATAG CAGGCTACCG ACTGACCGCG GGCCTGACCC GAGGAGGCCA GCCCAAGCAG
    TACAATGTGG GACCCTTGGC CTCCAAGTAT CCCCTGAGAA ATCTGCAGCC TGGGTCTGAG
    TACACCGTGA CCTTGGTGGC TGTGAAAGGG AACCAGCAGA GTCCCAAAGC CACCGGAGTC
    TTTACTACCC TGCAGCCTCT GCGCTCCATT CCACCTTACA ACACCGAGGT GACAGAGACC
    ACAATTGTGA TCACCTGGAC CCCCGCTCCA AGGATTGGCT TCAAGCTGGG TGTACGACCG
    AGCCAGGGAG GTGAGGCACC CCGAGAAGTG ACTTCAGACT CTGGGAGCAT TGTTGTGTCT
    GGCTTGACTC CAGGCGTGGA ATACACTTAC ACCATCCAAG TCCTGCGAGA TGGCCAGGAG
    AGAGATGCAC CGATTGTCAA CAGAGTAGTG ACACCGCTGT CTCCACCGAC CAACTTGCAT
    CTGGAGGCAA ACCCTGACAC TGGAGTGCTT ACTGTCTCCT GGGAGAGGAG CACTACCCCA
    GATATCACTG GCTACAGAAT AACTACTACC CCCACGAACG GGCAGCAGGG GACCTCTCTG
    GAAGAAGTGG TCCATGCTGA TCAGAGTTCC TGCACTTTTG AGAACCTGAA TCCTGGCCTG
    GAGTACAACG TCAGTGTTTA CACTGTCAAA GATGACAAGG AAAGTGCCCC TATCTCTGAT
    ACCGTTGTCC CAGAGGTGCC CCAGCTCACT GACCTAAGCT TTGTTGATAT AACTGATTCA
    AGCATCGGCC TGAGGTGGAC CCCGCTAAAC TCTTCCACCA TTATCGGGTA CCGAATCACA
    GTAGTTGCGG CAGGAGAAGG GATCCCTATT TTTGAAGATT TTGTGGACTC CTCAGTAGGA
    TACTACACAG TTACAGGGCT GGAGCCTGGC ATTGACTATG ACATCAGCGT TATCACTCTC
    ATTAATGGCG GAGAGAGTGC CCCTACTACA CTGACACAGC AAACGGCTGT CCCTCCTCCC
    ACGGATCTGC GATTCACCAA TATCGGTCCA GACACGATGC GGGTCACTTG GGCCCCGCCT
    CCGTCCATCG AGCTGACCAA CCTCTTGGTG CGCTACTCAC CCGTGAAGAA TGAAGAGGAC
    GTTGCAGAGC TATCCATTTC ACCTTCAGAC AATGCCGTGG TCCTAACAAA TCTCCTGCCT
    GGGACAGAAT ACTTAGTCAG TGTCTCCAGT GTCTACGAAC AACATGAGAG CATCCCTCTC
    CGGGGAAGGC AGAAAACAGG TCTCGATTCC CCAACTGGTT TTGATTCTTC TGATATCACC
    GCCAACTCAT TCACTGTCCA CTGGGTGGCT CCTCGGGCCC CCATCACCGG CTACATCATC
    CGCCATCACG CCGAGCATTC TGTCGGAAGA CCCAGGCAGG ATCGAGTGCC GCCCTCGCGG
    AATTCCATCA CCCTCACCAA CCTTAATCCG GGCACCGAGT ACGTTGTCAG CATCATTGCT
    GTTAATGGCA GAGAGGAGAG CCCGCCACTG ATTGGCCAGC AAGCCACAGT TTCTGATATT
    CCGAGAGATC TGGAGGTCAT TGCCTCCACC CCCACCAGCC TGCTCATCAG TTGGGAACCC
    CCTGCCGTCT CTGTGCGCTA TTACAGAATC ACCTACGGAG AGACAGGAGG AAATAGCCCT
    GTCCAGGAGT TCACTGTGCC CGGAAGCAAG TCCACAGCCA CCATCAACAA CATTAAACCA
    GGAGCAGACT ACACCATCAC CCTGTATGCT GTCACTGGCC GTGGGGACAG TCCAGCAAGC
    AGCAAGCCAG TTTCCATCAA TTATAAAACA GAAATTGACA AGCCGTCCCA GATGCAGGTG
    ACAGATGTCC AGGACAACAG CATCAGTGTC AGGTGGCTGC CTTCAACTTC TCCTGTGACA
    GGCTACAGAG TGACCACCAC TCCCAAAAAT GGCCTAGGAC CATCAAAAAC TAAAACTGCC
    AGTCCAGATC AAACAGAAAT GACCATTGAA GGTTTGCAAC CCACTGTGGA GTACGTGGTT
    AGTGTTTATG CTCAGAACCG GAACGGAGAA AGCCAGCCCC TGGTTCAAAC TGCAGTGACC
    ACCATTCCTG CGCCCACCAA TCTGAAGTTC AGTCAGGTGA CACCCACCAG CTTTACTGCC
    CAGTGGATAG CACCCAGTGT TCAGCTCACT GGCTACCGGG TGCGGGTGAA CCCGAAAGAG
    AAGACAGGAC CAATGAAAGA AATCAACCTT TCTCCAGACA GCTCATCGGT GATTGTGTCA
    GGACTCATGG TGGCCACTAA ATACGAAGTC AGTGTCTATG CTCTCAAGGA CACACTGACA
    AGCAGACCAG CCCAGGGAGT CATCACTACT CTGGAGAATG TTAGCCCTCC AAGAAGGGCC
    CGTGTGACGG ACGCTACAGA GACCACCATC ACTATTAGCT GGAGAACAAA GACAGAGACA
    ATCACTGGCT TCCAAGTCGA TGCCATCCCA GCCAATGGCC AGACCCCAGT TCAGAGGAGC
    ATCAGCCCGG ATGTTAGAAG CTACACCATT ACAGGTTTAC AGCCAGGCAC TGACTACAAG
    ATCCACCTGT ACACTCTGAA CGACAATGCC CGGAGCTCGC CCGTGATCAT CGATGCCTCC
    ACCGCCATCG ACGCACCATC CAACCTGCGG TTCCTGACCA CCACACCCAA CTCCTTGCTG
    GTGTCATGGC AGGCGCCCCG TGCCAGGATT ACTGGCTACA TTATCAAGTA TGAGAAGCCT
    GGATCCCCTC CCAGAGAAGT GGTCCCTCGG CCCCGCCCTG GTGTCACGGA GGCCACCATT
    ACTGGTCTGG AGCCAGGAAC CGAGTACACC ATCTACGTCA TTGCCCTGAA GAACAATCAG
    AAGAGTGAGC CCCTGATTGG GAGGAAGAAG ACAGATGAGC TTCCCCAACT GGTTACCCTT
    CCACACCCCA ATCTTCATGG ACCAGAGATC TTGGATGTTC CCTCCACAGT TCAAAAGACC
    CCCTTCATCA CCAACCCTGG GTATGACACC GAAAATGGTA TTCAGCTTCC TGGCACAACC
    CACCAGCAAC CCAGTGTTGG GCAACAAATG ATCTTTGAGG AACATGGCTT TAGGCGGACA
    ACGCCACCCA CTGCGGCCAC CCCCGTCAGG CTTAGGCCAA GACCATACCT GCCGAATGTA
    GATGAGGAGG TCCAAATCGG TCATGTTCCC AGGGGAGACG TAGACTACCA CCTCTATCCT
    CATGTTCCGG GCCTCAATCC AAATGCCTCT ACAGGACAGG AAGCTCTCTC TCAGACAACC
    ATCTCTTGGA CGCCGTTCCA GGAGAGTTCT GAGTACATCA TTTCATGCCA ACCAGTTGGC
    ACCGACGAAG AGCCCTTACA GTTCCAAGTT CCTGGAACTT CTACCAGTGC GACTCTGACT
    GGCCTTACCA GAGGGGTCAC CTACAACATC ATAGTGGAGG CACTGCAGAA CCAGAGGAGG
    CACAAGGTTC GGGAAGAGGT TGTGACTGTG GGCAACGCTG TCAGCGAAGG CCTGAACCAG
    CCTACAGATG ACTCATGCTT TGACCCTTAC ACGGTTTCCC ATTACGCCAT TGGAGAGGAG
    TGGGAGCGGT TGTCTGACGC TGGCTTTAAG CTCACATGCC AGTGCTTGGG CTTTGGCAGT
    GGTCATTTCA GATGCGATTC ATCTAAATGG TGCCATGACA ACGGTGTCAA CTACAAGATC
    GGAGAGAAGT GGGATCGGCA GGGAGAAAAT GGCCAGCGGA TGAGCTGCAC ATGCCTCGGG
    AATGGAAAGG GAGAATTCAA GTGTGATCCC CATGAAGCAA CGTGCTATGA CGATGGGAAG
    ACCTACCATG TAGGAGAACA GTGGCAGAAA GAATATCTCG GAGCCATTTG CTCCTGCACG
    TGTTTCGGAG GCCAGCGGGG CTGGCGCTGT GACAACTGCC GTAGACCTGG GGCTGCTGAA
    CCCAGTCCCG ATGGCACCAC CGGCCACACC TACAACCAGT ATACACAGAG ATACAATCAG
    AGAACAAACA CTAACGTAAA TTGCCCCATT GAGTGCTTCA TGCCGCTAGA TGTGCAAGCT
    GACAGAGACG ATTCTCGAGA GTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006495762.1
    CDS248..7411
    Translation

    Target ORF information:

    RefSeq Version XM_006495699.3
    Organism Mus musculus(house mouse)
    Definition Mus musculus fibronectin 1 (Fn1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006495699.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    ATGCTCAGGG GTCCGGGACC CGGGCGGCTG CTGCTGCTGG CAGTCCTGTG CCTGGGGACC 
    TCGGTGCGCT GCACCGAAGC CGGGAAGAGC AAGAGGCAGG CTCAGCAAAT CGTGCAGCCT
    CAATCCCCGG TGGCTGTCAG TCAGAGCAAG CCTGGCTGTT TTGACAATGG GAAGCACTAT
    CAGATAAATC AGCAGTGGGA ACGGACCTAC CTAGGCAACG CCCTGGTTTG TACCTGCTAT
    GGAGGAAGCC GGGGTTTTAA CTGCGAGAGC AAGCCTGAGC CTGAAGAGAC TTGCTTTGAC
    AAATACACTG GGAACACTTA CAAAGTGGGT GACACTTATG AGCGCCCTAA AGATTCCATG
    ATCTGGGACT GTACCTGCAT CGGGGCTGGG AGAGGCAGGA TCAGCTGTAC CATTGCAAAT
    CGCTGCCATG AAGGGGGTCA GTCCTACAAG ATTGGCGACA AGTGGAGGAG GCCACATGAG
    ACTGGTGGCT ACATGTTAGA GTGTCTGTGT CTGGGAAATG GAAAAGGGGA ATGGACCTGC
    AAACCTATAG CTGAGAAGTG TTTTGATCAT GCTGCTGGGA CGTCCTACGT CGTGGGGGAG
    ACCTGGGAAA AGCCCTACCA AGGCTGGATG ATGGTGGACT GTACTTGTCT AGGCGAAGGC
    AATGGACGCA TCACCTGTAC CTCCAGAAAC AGATGCAACG ATCAGGACAC CCGGACATCC
    TATAGGATTG GAGACACGTG GAGCAAGAAG GACAACCGAG GAAACCTGCT TCAGTGTGTC
    TGCACAGGCA ATGGCAGAGG GGAGTGGAAG TGTGAGCGAC ATGCTCTACA AAGTGCTTCA
    GCCGGATCTG GCTCCTTCAC TGATGTCCGA ACAGCTATTT ACCAACCGCA GACTCACCCC
    CAGCCCGCTC CCTACGGCCA CTGTGTCACC GACAGTGGTG TGGTCTACTC TGTGGGAATG
    CAGTGGCTGA AGTCGCAAGG AAACAAGCAA ATGCTGTGCA CGTGCCTGGG CAATGGCGTC
    AGCTGCCAGG AGACAGCCGT GACCCAGACT TATGGTGGCA ATTCAAACGG GGAGCCCTGT
    GTCCTCCCGT TCACCTACAA CGGTAGGACC TTCTATTCCT GCACCACCGA AGGGCGGCAA
    GACGGACATC TGTGGTGTAG CACAACTTCC AATTACGAAC AAGACCAGAA GTATTCCTTC
    TGCACAGACC ATGCGGTTTT GGTTCAGACT CGAGGCGGAA ATTCCAATGG TGCTCTGTGC
    CACTTCCCCT TCCTGTACAA CAACCGGAAT TACACCGACT GTACTTCTGA GGGTCGCAGG
    GACAACATGA AATGGTGCGG CACCACCCAG AACTACGATG CCGATCAGAA GTTTGGATTC
    TGCCCAATGG CTGCCCACGA GGAGATCTGC ACAACCAATG AAGGGGTCAT GTATCGCATT
    GGGGATCAGT GGGATAAGCA GCATGACCTG GGCCACATGA TGAGGTGCAC GTGTGTGGGG
    AACGGTCGTG GAGAATGGGC CTGCATCCCC TACTCCCAGC TCCGAGACCA GTGCATCGTT
    GATGACATTA CTTACAATGT GAACGACACG TTCCACAAGC GTCACGAGGA GGGACATATG
    CTGAACTGTA CCTGCTTTGG TCAGGGCCGG GGCAGATGGA AGTGTGACCC CATTGACCAG
    TGCCAAGATT CAGAGACCCG GACATTTTAC CAGATTGGTG ACTCCTGGGA GAAGTTTGTG
    CATGGTGTCC GATACCAGTG TTACTGCTAC GGCCGTGGCA TCGGGGAGTG GCACTGTCAA
    CCTCTGCAGA CCTACCCAGG CACAACTGGA CCTGTCCAAG TAATTATCAC GGAGACCCCC
    AGCCAGCCCA ATTCCCACCC CATCCAGTGG AATGCCCCGG AGCCTTCACA CATCACCAAG
    TACATTCTCA GATGGAGACC TAAAACCTCT ACGGGTCGCT GGAAGGAAGC TACCATTCCT
    GGCCACCTTA ACTCCTATAC CATCAAAGGC CTGACCCCAG GTGTGATCTA TGAGGGACAG
    CTCATCAGCA TCCAGCAGTA TGGCCACAGA GAAGTGACAC GCTTCGACTT CACCACCAGC
    GCCAGCACCC CTGTGACCAG CAACACGGTG ACCGGAGAGA CTGCGCCCTA CTCTCCTGTT
    GTGGCCACTT CTGAATCTGT AACTGAAATC ACAGCCAGCA GCTTTGTGGT CTCATGGGTC
    TCAGCCTCCG ACACCGTGTC AGGCTTCCGG GTGGAGTATG AGCTGAGCGA GGAGGGAGAT
    GAACCACAGT ACCTTGATCT CCCAAGCACG GCCACTTCCG TGAACATTCC TGACCTGCTC
    CCGGGCAGAA AGTACATTGT CAATGTCTAT CAGATATCTG AGGAGGGAAA ACAGAGCTTG
    ATCCTGTCTA CCTCACAGAC TACAGCACCT GACGCTCCTC CAGACCCTAC CGTGGACCAG
    GTTGATGATA CTTCCATTGT TGTTCGGTGG AGTAGACCCC AGGCACCTAT CACAGGGTAT
    AGAATTGTCT ATTCACCTTC AGTAGAAGGC AGTAGCACAG AGCTCAACCT CCCTGAAACG
    GCCAACTCCG TCACCCTCAG CGACCTGCAG CCCGGTGTTC AGTACAACAT CACTATCTAT
    GCTGTGGAGG AGAACCAGGA GAGCACACCC GTTTTCATCC AACAAGAGAC CACTGGCACC
    CCAAGATCTG ATAACGTCCC CCCTCCGACG GACCTACAGT TTGTGGAACT GACTGATGTG
    AAAGTCACCA TCATGTGGAC CCCTCCTGAT AGTGTGGTGT CTGGATACCG TGTGGAGGTC
    CTGCCTGTCA GCCTGCCCGG GGAACATGGG CAGAGGCTGC CTGTCAACAG AAATACCTTT
    GCTGAAATCA CTGGGCTGTC CCCTGGGGTC ACGTACCTCT TCAAAGTCTT TGCTGTGCAC
    CAGGGCAGGG AAAGCAATCC TCTGACGGCA CAACAGACCA CCAAACTCGA CGCTCCCACT
    AACCTCCAGT TTGTCAATGA AACTGACAGA ACAGTTCTGG TAACGTGGAC TCCACCTCGA
    GCCCGTATAG CAGGCTACCG ACTGACCGCG GGCCTGACCC GAGGAGGCCA GCCCAAGCAG
    TACAATGTGG GACCCTTGGC CTCCAAGTAT CCCCTGAGAA ATCTGCAGCC TGGGTCTGAG
    TACACCGTGA CCTTGGTGGC TGTGAAAGGG AACCAGCAGA GTCCCAAAGC CACCGGAGTC
    TTTACTACCC TGCAGCCTCT GCGCTCCATT CCACCTTACA ACACCGAGGT GACAGAGACC
    ACAATTGTGA TCACCTGGAC CCCCGCTCCA AGGATTGGCT TCAAGCTGGG TGTACGACCG
    AGCCAGGGAG GTGAGGCACC CCGAGAAGTG ACTTCAGACT CTGGGAGCAT TGTTGTGTCT
    GGCTTGACTC CAGGCGTGGA ATACACTTAC ACCATCCAAG TCCTGCGAGA TGGCCAGGAG
    AGAGATGCAC CGATTGTCAA CAGAGTAGTG ACACCGCTGT CTCCACCGAC CAACTTGCAT
    CTGGAGGCAA ACCCTGACAC TGGAGTGCTT ACTGTCTCCT GGGAGAGGAG CACTACCCCA
    GATATCACTG GCTACAGAAT AACTACTACC CCCACGAACG GGCAGCAGGG GACCTCTCTG
    GAAGAAGTGG TCCATGCTGA TCAGAGTTCC TGCACTTTTG AGAACCTGAA TCCTGGCCTG
    GAGTACAACG TCAGTGTTTA CACTGTCAAA GATGACAAGG AAAGTGCCCC TATCTCTGAT
    ACCGTTGTCC CAGAGGTGCC CCAGCTCACT GACCTAAGCT TTGTTGATAT AACTGATTCA
    AGCATCGGCC TGAGGTGGAC CCCGCTAAAC TCTTCCACCA TTATCGGGTA CCGAATCACA
    GTAGTTGCGG CAGGAGAAGG GATCCCTATT TTTGAAGATT TTGTGGACTC CTCAGTAGGA
    TACTACACAG TTACAGGGCT GGAGCCTGGC ATTGACTATG ACATCAGCGT TATCACTCTC
    ATTAATGGCG GAGAGAGTGC CCCTACTACA CTGACACAGC AAACGGCTGT CCCTCCTCCC
    ACGGATCTGC GATTCACCAA TATCGGTCCA GACACGATGC GGGTCACTTG GGCCCCGCCT
    CCGTCCATCG AGCTGACCAA CCTCTTGGTG CGCTACTCAC CCGTGAAGAA TGAAGAGGAC
    GTTGCAGAGC TATCCATTTC ACCTTCAGAC AATGCCGTGG TCCTAACAAA TCTCCTGCCT
    GGGACAGAAT ACTTAGTCAG TGTCTCCAGT GTCTACGAAC AACATGAGAG CATCCCTCTC
    CGGGGAAGGC AGAAAACAGG TCTCGATTCC CCAACTGGTT TTGATTCTTC TGATATCACC
    GCCAACTCAT TCACTGTCCA CTGGGTGGCT CCTCGGGCCC CCATCACCGG CTACATCATC
    CGCCATCACG CCGAGCATTC TGTCGGAAGA CCCAGGCAGG ATCGAGTGCC GCCCTCGCGG
    AATTCCATCA CCCTCACCAA CCTTAATCCG GGCACCGAGT ACGTTGTCAG CATCATTGCT
    GTTAATGGCA GAGAGGAGAG CCCGCCACTG ATTGGCCAGC AAGCCACAGT TTCTGATATT
    CCGAGAGATC TGGAGGTCAT TGCCTCCACC CCCACCAGCC TGCTCATCAG TTGGGAACCC
    CCTGCCGTCT CTGTGCGCTA TTACAGAATC ACCTACGGAG AGACAGGAGG AAATAGCCCT
    GTCCAGGAGT TCACTGTGCC CGGAAGCAAG TCCACAGCCA CCATCAACAA CATTAAACCA
    GGAGCAGACT ACACCATCAC CCTGTATGCT GTCACTGGCC GTGGGGACAG TCCAGCAAGC
    AGCAAGCCAG TTTCCATCAA TTATAAAACA GAAATTGACA AGCCGTCCCA GATGCAGGTG
    ACAGATGTCC AGGACAACAG CATCAGTGTC AGGTGGCTGC CTTCAACTTC TCCTGTGACA
    GGCTACAGAG TGACCACCAC TCCCAAAAAT GGCCTAGGAC CATCAAAAAC TAAAACTGCC
    AGTCCAGATC AAACAGAAAT GACCATTGAA GGTTTGCAAC CCACTGTGGA GTACGTGGTT
    AGTGTTTATG CTCAGAACCG GAACGGAGAA AGCCAGCCCC TGGTTCAAAC TGCAGTGACC
    ACCATTCCTG CGCCCACCAA TCTGAAGTTC AGTCAGGTGA CACCCACCAG CTTTACTGCC
    CAGTGGATAG CACCCAGTGT TCAGCTCACT GGCTACCGGG TGCGGGTGAA CCCGAAAGAG
    AAGACAGGAC CAATGAAAGA AATCAACCTT TCTCCAGACA GCTCATCGGT GATTGTGTCA
    GGACTCATGG TGGCCACTAA ATACGAAGTC AGTGTCTATG CTCTCAAGGA CACACTGACA
    AGCAGACCAG CCCAGGGAGT CATCACTACT CTGGAGAATG TTAGCCCTCC AAGAAGGGCC
    CGTGTGACGG ACGCTACAGA GACCACCATC ACTATTAGCT GGAGAACAAA GACAGAGACA
    ATCACTGGCT TCCAAGTCGA TGCCATCCCA GCCAATGGCC AGACCCCAGT TCAGAGGAGC
    ATCAGCCCGG ATGTTAGAAG CTACACCATT ACAGGTTTAC AGCCAGGCAC TGACTACAAG
    ATCCACCTGT ACACTCTGAA CGACAATGCC CGGAGCTCGC CCGTGATCAT CGATGCCTCC
    ACCGCCATCG ACGCACCATC CAACCTGCGG TTCCTGACCA CCACACCCAA CTCCTTGCTG
    GTGTCATGGC AGGCGCCCCG TGCCAGGATT ACTGGCTACA TTATCAAGTA TGAGAAGCCT
    GGATCCCCTC CCAGAGAAGT GGTCCCTCGG CCCCGCCCTG GTGTCACGGA GGCCACCATT
    ACTGGTCTGG AGCCAGGAAC CGAGTACACC ATCTACGTCA TTGCCCTGAA GAACAATCAG
    AAGAGTGAGC CCCTGATTGG GAGGAAGAAG ACAGATGAGC TTCCCCAACT GGTTACCCTT
    CCACACCCCA ATCTTCATGG ACCAGAGATC TTGGATGTTC CCTCCACAGT TCAAAAGACC
    CCCTTCATCA CCAACCCTGG GTATGACACC GAAAATGGTA TTCAGCTTCC TGGCACAACC
    CACCAGCAAC CCAGTGTTGG GCAACAAATG ATCTTTGAGG AACATGGCTT TAGGCGGACA
    ACGCCACCCA CTGCGGCCAC CCCCGTCAGG CTTAGGCCAA GACCATACCT GCCGAATGTA
    GATGAGGAGG TCCAAATCGG TCATGTTCCC AGGGGAGACG TAGACTACCA CCTCTATCCT
    CATGTTCCGG GCCTCAATCC AAATGCCTCT ACAGGACAGG AAGCTCTCTC TCAGACAACC
    ATCTCTTGGA CGCCGTTCCA GGAGAGTTCT GAGTACATCA TTTCATGCCA ACCAGTTGGC
    ACCGACGAAG AGCCCTTACA GTTCCAAGTT CCTGGAACTT CTACCAGTGC GACTCTGACT
    GGCCTTACCA GAGGGGTCAC CTACAACATC ATAGTGGAGG CACTGCAGAA CCAGAGGAGG
    CACAAGGTTC GGGAAGAGGT TGTGACTGTG GGCAACGCTG TCAGCGAAGG CCTGAACCAG
    CCTACAGATG ACTCATGCTT TGACCCTTAC ACGGTTTCCC ATTACGCCAT TGGAGAGGAG
    TGGGAGCGGT TGTCTGACGC TGGCTTTAAG CTCACATGCC AGTGCTTGGG CTTTGGCAGT
    GGTCATTTCA GATGCGATTC ATCTAAATGG TGCCATGACA ACGGTGTCAA CTACAAGATC
    GGAGAGAAGT GGGATCGGCA GGGAGAAAAT GGCCAGCGGA TGAGCTGCAC ATGCCTCGGG
    AATGGAAAGG GAGAATTCAA GTGTGATCCC CATGAAGCAA CGTGCTATGA CGATGGGAAG
    ACCTACCATG TAGGAGAACA GTGGCAGAAA GAATATCTCG GAGCCATTTG CTCCTGCACG
    TGTTTCGGAG GCCAGCGGGG CTGGCGCTGT GACAACTGCC GTAGACCTGG GGCTGCTGAA
    CCCAGTCCCG ATGGCACCAC CGGCCACACC TACAACCAGT ATACACAGAG ATACAATCAG
    AGAACAAACA CTAACGTAAA TTGCCCCATT GAGTGCTTCA TGCCGCTAGA TGTGCAAGCT
    GACAGAGACG ATTCTCGAGA GTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu27268
    Clone ID Related Accession (Same CDS sequence) XM_006495700.3 , XM_006495700.4
    Accession Version XM_006495700.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7074bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-21
    Organism Mus musculus(house mouse)
    Product fibronectin isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006495700.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    ATGCTCAGGG GTCCGGGACC CGGGCGGCTG CTGCTGCTGG CAGTCCTGTG CCTGGGGACC 
    TCGGTGCGCT GCACCGAAGC CGGGAAGAGC AAGAGGCAGG CTCAGCAAAT CGTGCAGCCT
    CAATCCCCGG TGGCTGTCAG TCAGAGCAAG CCTGGCTGTT TTGACAATGG GAAGCACTAT
    CAGATAAATC AGCAGTGGGA ACGGACCTAC CTAGGCAACG CCCTGGTTTG TACCTGCTAT
    GGAGGAAGCC GGGGTTTTAA CTGCGAGAGC AAGCCTGAGC CTGAAGAGAC TTGCTTTGAC
    AAATACACTG GGAACACTTA CAAAGTGGGT GACACTTATG AGCGCCCTAA AGATTCCATG
    ATCTGGGACT GTACCTGCAT CGGGGCTGGG AGAGGCAGGA TCAGCTGTAC CATTGCAAAT
    CGCTGCCATG AAGGGGGTCA GTCCTACAAG ATTGGCGACA AGTGGAGGAG GCCACATGAG
    ACTGGTGGCT ACATGTTAGA GTGTCTGTGT CTGGGAAATG GAAAAGGGGA ATGGACCTGC
    AAACCTATAG CTGAGAAGTG TTTTGATCAT GCTGCTGGGA CGTCCTACGT CGTGGGGGAG
    ACCTGGGAAA AGCCCTACCA AGGCTGGATG ATGGTGGACT GTACTTGTCT AGGCGAAGGC
    AATGGACGCA TCACCTGTAC CTCCAGAAAC AGATGCAACG ATCAGGACAC CCGGACATCC
    TATAGGATTG GAGACACGTG GAGCAAGAAG GACAACCGAG GAAACCTGCT TCAGTGTGTC
    TGCACAGGCA ATGGCAGAGG GGAGTGGAAG TGTGAGCGAC ATGCTCTACA AAGTGCTTCA
    GCCGGATCTG GCTCCTTCAC TGATGTCCGA ACAGCTATTT ACCAACCGCA GACTCACCCC
    CAGCCCGCTC CCTACGGCCA CTGTGTCACC GACAGTGGTG TGGTCTACTC TGTGGGAATG
    CAGTGGCTGA AGTCGCAAGG AAACAAGCAA ATGCTGTGCA CGTGCCTGGG CAATGGCGTC
    AGCTGCCAGG AGACAGCCGT GACCCAGACT TATGGTGGCA ATTCAAACGG GGAGCCCTGT
    GTCCTCCCGT TCACCTACAA CGGTAGGACC TTCTATTCCT GCACCACCGA AGGGCGGCAA
    GACGGACATC TGTGGTGTAG CACAACTTCC AATTACGAAC AAGACCAGAA GTATTCCTTC
    TGCACAGACC ATGCGGTTTT GGTTCAGACT CGAGGCGGAA ATTCCAATGG TGCTCTGTGC
    CACTTCCCCT TCCTGTACAA CAACCGGAAT TACACCGACT GTACTTCTGA GGGTCGCAGG
    GACAACATGA AATGGTGCGG CACCACCCAG AACTACGATG CCGATCAGAA GTTTGGATTC
    TGCCCAATGG CTGCCCACGA GGAGATCTGC ACAACCAATG AAGGGGTCAT GTATCGCATT
    GGGGATCAGT GGGATAAGCA GCATGACCTG GGCCACATGA TGAGGTGCAC GTGTGTGGGG
    AACGGTCGTG GAGAATGGGC CTGCATCCCC TACTCCCAGC TCCGAGACCA GTGCATCGTT
    GATGACATTA CTTACAATGT GAACGACACG TTCCACAAGC GTCACGAGGA GGGACATATG
    CTGAACTGTA CCTGCTTTGG TCAGGGCCGG GGCAGATGGA AGTGTGACCC CATTGACCAG
    TGCCAAGATT CAGAGACCCG GACATTTTAC CAGATTGGTG ACTCCTGGGA GAAGTTTGTG
    CATGGTGTCC GATACCAGTG TTACTGCTAC GGCCGTGGCA TCGGGGAGTG GCACTGTCAA
    CCTCTGCAGA CCTACCCAGG CACAACTGGA CCTGTCCAAG TAATTATCAC GGAGACCCCC
    AGCCAGCCCA ATTCCCACCC CATCCAGTGG AATGCCCCGG AGCCTTCACA CATCACCAAG
    TACATTCTCA GATGGAGACC TAAAACCTCT ACGGGTCGCT GGAAGGAAGC TACCATTCCT
    GGCCACCTTA ACTCCTATAC CATCAAAGGC CTGACCCCAG GTGTGATCTA TGAGGGACAG
    CTCATCAGCA TCCAGCAGTA TGGCCACAGA GAAGTGACAC GCTTCGACTT CACCACCAGC
    GCCAGCACCC CTGTGACCAG CAACACGGTG ACCGGAGAGA CTGCGCCCTA CTCTCCTGTT
    GTGGCCACTT CTGAATCTGT AACTGAAATC ACAGCCAGCA GCTTTGTGGT CTCATGGGTC
    TCAGCCTCCG ACACCGTGTC AGGCTTCCGG GTGGAGTATG AGCTGAGCGA GGAGGGAGAT
    GAACCACAGT ACCTTGATCT CCCAAGCACG GCCACTTCCG TGAACATTCC TGACCTGCTC
    CCGGGCAGAA AGTACATTGT CAATGTCTAT CAGATATCTG AGGAGGGAAA ACAGAGCTTG
    ATCCTGTCTA CCTCACAGAC TACAGCACCT GACGCTCCTC CAGACCCTAC CGTGGACCAG
    GTTGATGATA CTTCCATTGT TGTTCGGTGG AGTAGACCCC AGGCACCTAT CACAGGGTAT
    AGAATTGTCT ATTCACCTTC AGTAGAAGGC AGTAGCACAG AGCTCAACCT CCCTGAAACG
    GCCAACTCCG TCACCCTCAG CGACCTGCAG CCCGGTGTTC AGTACAACAT CACTATCTAT
    GCTGTGGAGG AGAACCAGGA GAGCACACCC GTTTTCATCC AACAAGAGAC CACTGGCACC
    CCAAGATCTG ATAACGTCCC CCCTCCGACG GACCTACAGT TTGTGGAACT GACTGATGTG
    AAAGTCACCA TCATGTGGAC CCCTCCTGAT AGTGTGGTGT CTGGATACCG TGTGGAGGTC
    CTGCCTGTCA GCCTGCCCGG GGAACATGGG CAGAGGCTGC CTGTCAACAG AAATACCTTT
    GCTGAAATCA CTGGGCTGTC CCCTGGGGTC ACGTACCTCT TCAAAGTCTT TGCTGTGCAC
    CAGGGCAGGG AAAGCAATCC TCTGACGGCA CAACAGACCA CCAAACTCGA CGCTCCCACT
    AACCTCCAGT TTGTCAATGA AACTGACAGA ACAGTTCTGG TAACGTGGAC TCCACCTCGA
    GCCCGTATAG CAGGCTACCG ACTGACCGCG GGCCTGACCC GAGGAGGCCA GCCCAAGCAG
    TACAATGTGG GACCCTTGGC CTCCAAGTAT CCCCTGAGAA ATCTGCAGCC TGGGTCTGAG
    TACACCGTGA CCTTGGTGGC TGTGAAAGGG AACCAGCAGA GTCCCAAAGC CACCGGAGTC
    TTTACTACCC TGCAGCCTCT GCGCTCCATT CCACCTTACA ACACCGAGGT GACAGAGACC
    ACAATTGTGA TCACCTGGAC CCCCGCTCCA AGGATTGGCT TCAAGCTGGG TGTACGACCG
    AGCCAGGGAG GTGAGGCACC CCGAGAAGTG ACTTCAGACT CTGGGAGCAT TGTTGTGTCT
    GGCTTGACTC CAGGCGTGGA ATACACTTAC ACCATCCAAG TCCTGCGAGA TGGCCAGGAG
    AGAGATGCAC CGATTGTCAA CAGAGTAGTG ACACCGCTGT CTCCACCGAC CAACTTGCAT
    CTGGAGGCAA ACCCTGACAC TGGAGTGCTT ACTGTCTCCT GGGAGAGGAG CACTACCCCA
    GATATCACTG GCTACAGAAT AACTACTACC CCCACGAACG GGCAGCAGGG GACCTCTCTG
    GAAGAAGTGG TCCATGCTGA TCAGAGTTCC TGCACTTTTG AGAACCTGAA TCCTGGCCTG
    GAGTACAACG TCAGTGTTTA CACTGTCAAA GATGACAAGG AAAGTGCCCC TATCTCTGAT
    ACCGTTGTCC CAGAGGTGCC CCAGCTCACT GACCTAAGCT TTGTTGATAT AACTGATTCA
    AGCATCGGCC TGAGGTGGAC CCCGCTAAAC TCTTCCACCA TTATCGGGTA CCGAATCACA
    GTAGTTGCGG CAGGAGAAGG GATCCCTATT TTTGAAGATT TTGTGGACTC CTCAGTAGGA
    TACTACACAG TTACAGGGCT GGAGCCTGGC ATTGACTATG ACATCAGCGT TATCACTCTC
    ATTAATGGCG GAGAGAGTGC CCCTACTACA CTGACACAGC AAACGGCTGT CCCTCCTCCC
    ACGGATCTGC GATTCACCAA TATCGGTCCA GACACGATGC GGGTCACTTG GGCCCCGCCT
    CCGTCCATCG AGCTGACCAA CCTCTTGGTG CGCTACTCAC CCGTGAAGAA TGAAGAGGAC
    GTTGCAGAGC TATCCATTTC ACCTTCAGAC AATGCCGTGG TCCTAACAAA TCTCCTGCCT
    GGGACAGAAT ACTTAGTCAG TGTCTCCAGT GTCTACGAAC AACATGAGAG CATCCCTCTC
    CGGGGAAGGC AGAAAACAGG TCTCGATTCC CCAACTGGTT TTGATTCTTC TGATATCACC
    GCCAACTCAT TCACTGTCCA CTGGGTGGCT CCTCGGGCCC CCATCACCGG CTACATCATC
    CGCCATCACG CCGAGCATTC TGTCGGAAGA CCCAGGCAGG ATCGAGTGCC GCCCTCGCGG
    AATTCCATCA CCCTCACCAA CCTTAATCCG GGCACCGAGT ACGTTGTCAG CATCATTGCT
    GTTAATGGCA GAGAGGAGAG CCCGCCACTG ATTGGCCAGC AAGCCACAGT TTCTGATATT
    CCGAGAGATC TGGAGGTCAT TGCCTCCACC CCCACCAGCC TGCTCATCAG TTGGGAACCC
    CCTGCCGTCT CTGTGCGCTA TTACAGAATC ACCTACGGAG AGACAGGAGG AAATAGCCCT
    GTCCAGGAGT TCACTGTGCC CGGAAGCAAG TCCACAGCCA CCATCAACAA CATTAAACCA
    GGAGCAGACT ACACCATCAC CCTGTATGCT GTCACTGGCC GTGGGGACAG TCCAGCAAGC
    AGCAAGCCAG TTTCCATCAA TTATAAAACA GAAATTGACA AGCCGTCCCA GATGCAGGTG
    ACAGATGTCC AGGACAACAG CATCAGTGTC AGGTGGCTGC CTTCAACTTC TCCTGTGACA
    GGCTACAGAG TGACCACCAC TCCCAAAAAT GGCCTAGGAC CATCAAAAAC TAAAACTGCC
    AGTCCAGATC AAACAGAAAT GACCATTGAA GGTTTGCAAC CCACTGTGGA GTACGTGGTT
    AGTGTTTATG CTCAGAACCG GAACGGAGAA AGCCAGCCCC TGGTTCAAAC TGCAGTGACC
    AACATTGATC GCCCTAAAGG ACTGGCATTC ACTGATGTGG ATGTCGATTC CATCAAAATT
    GCTTGGGAAA GCCCACAGGG GCAAGTTTCC AGGTACAGGG TGACCTACTC GAGCCCTGAG
    GATGGAATCC GGGAGCTTTT CCCTGCACCT GATGGTGAAG ACGACACTGC AGAGCTGCAG
    GGCCTCAGGC CGGGGTCTGA GTACACAGTC AGTGTGGTTG CCTTGCACGA TGATATGGAG
    AGCCAGCCCC TGATTGGAAT CCAGTCCACA GCCATTCCTG CGCCCACCAA TCTGAAGTTC
    AGTCAGGTGA CACCCACCAG CTTTACTGCC CAGTGGATAG CACCCAGTGT TCAGCTCACT
    GGCTACCGGG TGCGGGTGAA CCCGAAAGAG AAGACAGGAC CAATGAAAGA AATCAACCTT
    TCTCCAGACA GCTCATCGGT GATTGTGTCA GGACTCATGG TGGCCACTAA ATACGAAGTC
    AGTGTCTATG CTCTCAAGGA CACACTGACA AGCAGACCAG CCCAGGGAGT CATCACTACT
    CTGGAGAATG TTAGCCCTCC AAGAAGGGCC CGTGTGACGG ACGCTACAGA GACCACCATC
    ACTATTAGCT GGAGAACAAA GACAGAGACA ATCACTGGCT TCCAAGTCGA TGCCATCCCA
    GCCAATGGCC AGACCCCAGT TCAGAGGAGC ATCAGCCCGG ATGTTAGAAG CTACACCATT
    ACAGGTTTAC AGCCAGGCAC TGACTACAAG ATCCACCTGT ACACTCTGAA CGACAATGCC
    CGGAGCTCGC CCGTGATCAT CGATGCCTCC ACCGCCATCG ACGCACCATC CAACCTGCGG
    TTCCTGACCA CCACACCCAA CTCCTTGCTG GTGTCATGGC AGGCGCCCCG TGCCAGGATT
    ACTGGCTACA TTATCAAGTA TGAGAAGCCT GGATCCCCTC CCAGAGAAGT GGTCCCTCGG
    CCCCGCCCTG GTGTCACGGA GGCCACCATT ACTGGTCTGG AGCCAGGAAC CGAGTACACC
    ATCTACGTCA TTGCCCTGAA GAACAATCAG AAGAGTGAGC CCCTGATTGG GAGGAAGAAG
    ACAGGACAGG AAGCTCTCTC TCAGACAACC ATCTCTTGGA CGCCGTTCCA GGAGAGTTCT
    GAGTACATCA TTTCATGCCA ACCAGTTGGC ACCGACGAAG AGCCCTTACA GTTCCAAGTT
    CCTGGAACTT CTACCAGTGC GACTCTGACT GGCCTTACCA GAGGGGTCAC CTACAACATC
    ATAGTGGAGG CACTGCAGAA CCAGAGGAGG CACAAGGTTC GGGAAGAGGT TGTGACTGTG
    GGCAACGCTG TCAGCGAAGG CCTGAACCAG CCTACAGATG ACTCATGCTT TGACCCTTAC
    ACGGTTTCCC ATTACGCCAT TGGAGAGGAG TGGGAGCGGT TGTCTGACGC TGGCTTTAAG
    CTCACATGCC AGTGCTTGGG CTTTGGCAGT GGTCATTTCA GATGCGATTC ATCTAAATGG
    TGCCATGACA ACGGTGTCAA CTACAAGATC GGAGAGAAGT GGGATCGGCA GGGAGAAAAT
    GGCCAGCGGA TGAGCTGCAC ATGCCTCGGG AATGGAAAGG GAGAATTCAA GTGTGATCCC
    CATGAAGCAA CGTGCTATGA CGATGGGAAG ACCTACCATG TAGGAGAACA GTGGCAGAAA
    GAATATCTCG GAGCCATTTG CTCCTGCACG TGTTTCGGAG GCCAGCGGGG CTGGCGCTGT
    GACAACTGCC GTAGACCTGG GGCTGCTGAA CCCAGTCCCG ATGGCACCAC CGGCCACACC
    TACAACCAGT ATACACAGAG ATACAATCAG AGAACAAACA CTAACGTAAA TTGCCCCATT
    GAGTGCTTCA TGCCGCTAGA TGTGCAAGCT GACAGAGACG ATTCTCGAGA GTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006495763.1
    CDS248..7321
    Translation

    Target ORF information:

    RefSeq Version XM_006495700.3
    Organism Mus musculus(house mouse)
    Definition Mus musculus fibronectin 1 (Fn1), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006495700.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    ATGCTCAGGG GTCCGGGACC CGGGCGGCTG CTGCTGCTGG CAGTCCTGTG CCTGGGGACC 
    TCGGTGCGCT GCACCGAAGC CGGGAAGAGC AAGAGGCAGG CTCAGCAAAT CGTGCAGCCT
    CAATCCCCGG TGGCTGTCAG TCAGAGCAAG CCTGGCTGTT TTGACAATGG GAAGCACTAT
    CAGATAAATC AGCAGTGGGA ACGGACCTAC CTAGGCAACG CCCTGGTTTG TACCTGCTAT
    GGAGGAAGCC GGGGTTTTAA CTGCGAGAGC AAGCCTGAGC CTGAAGAGAC TTGCTTTGAC
    AAATACACTG GGAACACTTA CAAAGTGGGT GACACTTATG AGCGCCCTAA AGATTCCATG
    ATCTGGGACT GTACCTGCAT CGGGGCTGGG AGAGGCAGGA TCAGCTGTAC CATTGCAAAT
    CGCTGCCATG AAGGGGGTCA GTCCTACAAG ATTGGCGACA AGTGGAGGAG GCCACATGAG
    ACTGGTGGCT ACATGTTAGA GTGTCTGTGT CTGGGAAATG GAAAAGGGGA ATGGACCTGC
    AAACCTATAG CTGAGAAGTG TTTTGATCAT GCTGCTGGGA CGTCCTACGT CGTGGGGGAG
    ACCTGGGAAA AGCCCTACCA AGGCTGGATG ATGGTGGACT GTACTTGTCT AGGCGAAGGC
    AATGGACGCA TCACCTGTAC CTCCAGAAAC AGATGCAACG ATCAGGACAC CCGGACATCC
    TATAGGATTG GAGACACGTG GAGCAAGAAG GACAACCGAG GAAACCTGCT TCAGTGTGTC
    TGCACAGGCA ATGGCAGAGG GGAGTGGAAG TGTGAGCGAC ATGCTCTACA AAGTGCTTCA
    GCCGGATCTG GCTCCTTCAC TGATGTCCGA ACAGCTATTT ACCAACCGCA GACTCACCCC
    CAGCCCGCTC CCTACGGCCA CTGTGTCACC GACAGTGGTG TGGTCTACTC TGTGGGAATG
    CAGTGGCTGA AGTCGCAAGG AAACAAGCAA ATGCTGTGCA CGTGCCTGGG CAATGGCGTC
    AGCTGCCAGG AGACAGCCGT GACCCAGACT TATGGTGGCA ATTCAAACGG GGAGCCCTGT
    GTCCTCCCGT TCACCTACAA CGGTAGGACC TTCTATTCCT GCACCACCGA AGGGCGGCAA
    GACGGACATC TGTGGTGTAG CACAACTTCC AATTACGAAC AAGACCAGAA GTATTCCTTC
    TGCACAGACC ATGCGGTTTT GGTTCAGACT CGAGGCGGAA ATTCCAATGG TGCTCTGTGC
    CACTTCCCCT TCCTGTACAA CAACCGGAAT TACACCGACT GTACTTCTGA GGGTCGCAGG
    GACAACATGA AATGGTGCGG CACCACCCAG AACTACGATG CCGATCAGAA GTTTGGATTC
    TGCCCAATGG CTGCCCACGA GGAGATCTGC ACAACCAATG AAGGGGTCAT GTATCGCATT
    GGGGATCAGT GGGATAAGCA GCATGACCTG GGCCACATGA TGAGGTGCAC GTGTGTGGGG
    AACGGTCGTG GAGAATGGGC CTGCATCCCC TACTCCCAGC TCCGAGACCA GTGCATCGTT
    GATGACATTA CTTACAATGT GAACGACACG TTCCACAAGC GTCACGAGGA GGGACATATG
    CTGAACTGTA CCTGCTTTGG TCAGGGCCGG GGCAGATGGA AGTGTGACCC CATTGACCAG
    TGCCAAGATT CAGAGACCCG GACATTTTAC CAGATTGGTG ACTCCTGGGA GAAGTTTGTG
    CATGGTGTCC GATACCAGTG TTACTGCTAC GGCCGTGGCA TCGGGGAGTG GCACTGTCAA
    CCTCTGCAGA CCTACCCAGG CACAACTGGA CCTGTCCAAG TAATTATCAC GGAGACCCCC
    AGCCAGCCCA ATTCCCACCC CATCCAGTGG AATGCCCCGG AGCCTTCACA CATCACCAAG
    TACATTCTCA GATGGAGACC TAAAACCTCT ACGGGTCGCT GGAAGGAAGC TACCATTCCT
    GGCCACCTTA ACTCCTATAC CATCAAAGGC CTGACCCCAG GTGTGATCTA TGAGGGACAG
    CTCATCAGCA TCCAGCAGTA TGGCCACAGA GAAGTGACAC GCTTCGACTT CACCACCAGC
    GCCAGCACCC CTGTGACCAG CAACACGGTG ACCGGAGAGA CTGCGCCCTA CTCTCCTGTT
    GTGGCCACTT CTGAATCTGT AACTGAAATC ACAGCCAGCA GCTTTGTGGT CTCATGGGTC
    TCAGCCTCCG ACACCGTGTC AGGCTTCCGG GTGGAGTATG AGCTGAGCGA GGAGGGAGAT
    GAACCACAGT ACCTTGATCT CCCAAGCACG GCCACTTCCG TGAACATTCC TGACCTGCTC
    CCGGGCAGAA AGTACATTGT CAATGTCTAT CAGATATCTG AGGAGGGAAA ACAGAGCTTG
    ATCCTGTCTA CCTCACAGAC TACAGCACCT GACGCTCCTC CAGACCCTAC CGTGGACCAG
    GTTGATGATA CTTCCATTGT TGTTCGGTGG AGTAGACCCC AGGCACCTAT CACAGGGTAT
    AGAATTGTCT ATTCACCTTC AGTAGAAGGC AGTAGCACAG AGCTCAACCT CCCTGAAACG
    GCCAACTCCG TCACCCTCAG CGACCTGCAG CCCGGTGTTC AGTACAACAT CACTATCTAT
    GCTGTGGAGG AGAACCAGGA GAGCACACCC GTTTTCATCC AACAAGAGAC CACTGGCACC
    CCAAGATCTG ATAACGTCCC CCCTCCGACG GACCTACAGT TTGTGGAACT GACTGATGTG
    AAAGTCACCA TCATGTGGAC CCCTCCTGAT AGTGTGGTGT CTGGATACCG TGTGGAGGTC
    CTGCCTGTCA GCCTGCCCGG GGAACATGGG CAGAGGCTGC CTGTCAACAG AAATACCTTT
    GCTGAAATCA CTGGGCTGTC CCCTGGGGTC ACGTACCTCT TCAAAGTCTT TGCTGTGCAC
    CAGGGCAGGG AAAGCAATCC TCTGACGGCA CAACAGACCA CCAAACTCGA CGCTCCCACT
    AACCTCCAGT TTGTCAATGA AACTGACAGA ACAGTTCTGG TAACGTGGAC TCCACCTCGA
    GCCCGTATAG CAGGCTACCG ACTGACCGCG GGCCTGACCC GAGGAGGCCA GCCCAAGCAG
    TACAATGTGG GACCCTTGGC CTCCAAGTAT CCCCTGAGAA ATCTGCAGCC TGGGTCTGAG
    TACACCGTGA CCTTGGTGGC TGTGAAAGGG AACCAGCAGA GTCCCAAAGC CACCGGAGTC
    TTTACTACCC TGCAGCCTCT GCGCTCCATT CCACCTTACA ACACCGAGGT GACAGAGACC
    ACAATTGTGA TCACCTGGAC CCCCGCTCCA AGGATTGGCT TCAAGCTGGG TGTACGACCG
    AGCCAGGGAG GTGAGGCACC CCGAGAAGTG ACTTCAGACT CTGGGAGCAT TGTTGTGTCT
    GGCTTGACTC CAGGCGTGGA ATACACTTAC ACCATCCAAG TCCTGCGAGA TGGCCAGGAG
    AGAGATGCAC CGATTGTCAA CAGAGTAGTG ACACCGCTGT CTCCACCGAC CAACTTGCAT
    CTGGAGGCAA ACCCTGACAC TGGAGTGCTT ACTGTCTCCT GGGAGAGGAG CACTACCCCA
    GATATCACTG GCTACAGAAT AACTACTACC CCCACGAACG GGCAGCAGGG GACCTCTCTG
    GAAGAAGTGG TCCATGCTGA TCAGAGTTCC TGCACTTTTG AGAACCTGAA TCCTGGCCTG
    GAGTACAACG TCAGTGTTTA CACTGTCAAA GATGACAAGG AAAGTGCCCC TATCTCTGAT
    ACCGTTGTCC CAGAGGTGCC CCAGCTCACT GACCTAAGCT TTGTTGATAT AACTGATTCA
    AGCATCGGCC TGAGGTGGAC CCCGCTAAAC TCTTCCACCA TTATCGGGTA CCGAATCACA
    GTAGTTGCGG CAGGAGAAGG GATCCCTATT TTTGAAGATT TTGTGGACTC CTCAGTAGGA
    TACTACACAG TTACAGGGCT GGAGCCTGGC ATTGACTATG ACATCAGCGT TATCACTCTC
    ATTAATGGCG GAGAGAGTGC CCCTACTACA CTGACACAGC AAACGGCTGT CCCTCCTCCC
    ACGGATCTGC GATTCACCAA TATCGGTCCA GACACGATGC GGGTCACTTG GGCCCCGCCT
    CCGTCCATCG AGCTGACCAA CCTCTTGGTG CGCTACTCAC CCGTGAAGAA TGAAGAGGAC
    GTTGCAGAGC TATCCATTTC ACCTTCAGAC AATGCCGTGG TCCTAACAAA TCTCCTGCCT
    GGGACAGAAT ACTTAGTCAG TGTCTCCAGT GTCTACGAAC AACATGAGAG CATCCCTCTC
    CGGGGAAGGC AGAAAACAGG TCTCGATTCC CCAACTGGTT TTGATTCTTC TGATATCACC
    GCCAACTCAT TCACTGTCCA CTGGGTGGCT CCTCGGGCCC CCATCACCGG CTACATCATC
    CGCCATCACG CCGAGCATTC TGTCGGAAGA CCCAGGCAGG ATCGAGTGCC GCCCTCGCGG
    AATTCCATCA CCCTCACCAA CCTTAATCCG GGCACCGAGT ACGTTGTCAG CATCATTGCT
    GTTAATGGCA GAGAGGAGAG CCCGCCACTG ATTGGCCAGC AAGCCACAGT TTCTGATATT
    CCGAGAGATC TGGAGGTCAT TGCCTCCACC CCCACCAGCC TGCTCATCAG TTGGGAACCC
    CCTGCCGTCT CTGTGCGCTA TTACAGAATC ACCTACGGAG AGACAGGAGG AAATAGCCCT
    GTCCAGGAGT TCACTGTGCC CGGAAGCAAG TCCACAGCCA CCATCAACAA CATTAAACCA
    GGAGCAGACT ACACCATCAC CCTGTATGCT GTCACTGGCC GTGGGGACAG TCCAGCAAGC
    AGCAAGCCAG TTTCCATCAA TTATAAAACA GAAATTGACA AGCCGTCCCA GATGCAGGTG
    ACAGATGTCC AGGACAACAG CATCAGTGTC AGGTGGCTGC CTTCAACTTC TCCTGTGACA
    GGCTACAGAG TGACCACCAC TCCCAAAAAT GGCCTAGGAC CATCAAAAAC TAAAACTGCC
    AGTCCAGATC AAACAGAAAT GACCATTGAA GGTTTGCAAC CCACTGTGGA GTACGTGGTT
    AGTGTTTATG CTCAGAACCG GAACGGAGAA AGCCAGCCCC TGGTTCAAAC TGCAGTGACC
    AACATTGATC GCCCTAAAGG ACTGGCATTC ACTGATGTGG ATGTCGATTC CATCAAAATT
    GCTTGGGAAA GCCCACAGGG GCAAGTTTCC AGGTACAGGG TGACCTACTC GAGCCCTGAG
    GATGGAATCC GGGAGCTTTT CCCTGCACCT GATGGTGAAG ACGACACTGC AGAGCTGCAG
    GGCCTCAGGC CGGGGTCTGA GTACACAGTC AGTGTGGTTG CCTTGCACGA TGATATGGAG
    AGCCAGCCCC TGATTGGAAT CCAGTCCACA GCCATTCCTG CGCCCACCAA TCTGAAGTTC
    AGTCAGGTGA CACCCACCAG CTTTACTGCC CAGTGGATAG CACCCAGTGT TCAGCTCACT
    GGCTACCGGG TGCGGGTGAA CCCGAAAGAG AAGACAGGAC CAATGAAAGA AATCAACCTT
    TCTCCAGACA GCTCATCGGT GATTGTGTCA GGACTCATGG TGGCCACTAA ATACGAAGTC
    AGTGTCTATG CTCTCAAGGA CACACTGACA AGCAGACCAG CCCAGGGAGT CATCACTACT
    CTGGAGAATG TTAGCCCTCC AAGAAGGGCC CGTGTGACGG ACGCTACAGA GACCACCATC
    ACTATTAGCT GGAGAACAAA GACAGAGACA ATCACTGGCT TCCAAGTCGA TGCCATCCCA
    GCCAATGGCC AGACCCCAGT TCAGAGGAGC ATCAGCCCGG ATGTTAGAAG CTACACCATT
    ACAGGTTTAC AGCCAGGCAC TGACTACAAG ATCCACCTGT ACACTCTGAA CGACAATGCC
    CGGAGCTCGC CCGTGATCAT CGATGCCTCC ACCGCCATCG ACGCACCATC CAACCTGCGG
    TTCCTGACCA CCACACCCAA CTCCTTGCTG GTGTCATGGC AGGCGCCCCG TGCCAGGATT
    ACTGGCTACA TTATCAAGTA TGAGAAGCCT GGATCCCCTC CCAGAGAAGT GGTCCCTCGG
    CCCCGCCCTG GTGTCACGGA GGCCACCATT ACTGGTCTGG AGCCAGGAAC CGAGTACACC
    ATCTACGTCA TTGCCCTGAA GAACAATCAG AAGAGTGAGC CCCTGATTGG GAGGAAGAAG
    ACAGGACAGG AAGCTCTCTC TCAGACAACC ATCTCTTGGA CGCCGTTCCA GGAGAGTTCT
    GAGTACATCA TTTCATGCCA ACCAGTTGGC ACCGACGAAG AGCCCTTACA GTTCCAAGTT
    CCTGGAACTT CTACCAGTGC GACTCTGACT GGCCTTACCA GAGGGGTCAC CTACAACATC
    ATAGTGGAGG CACTGCAGAA CCAGAGGAGG CACAAGGTTC GGGAAGAGGT TGTGACTGTG
    GGCAACGCTG TCAGCGAAGG CCTGAACCAG CCTACAGATG ACTCATGCTT TGACCCTTAC
    ACGGTTTCCC ATTACGCCAT TGGAGAGGAG TGGGAGCGGT TGTCTGACGC TGGCTTTAAG
    CTCACATGCC AGTGCTTGGG CTTTGGCAGT GGTCATTTCA GATGCGATTC ATCTAAATGG
    TGCCATGACA ACGGTGTCAA CTACAAGATC GGAGAGAAGT GGGATCGGCA GGGAGAAAAT
    GGCCAGCGGA TGAGCTGCAC ATGCCTCGGG AATGGAAAGG GAGAATTCAA GTGTGATCCC
    CATGAAGCAA CGTGCTATGA CGATGGGAAG ACCTACCATG TAGGAGAACA GTGGCAGAAA
    GAATATCTCG GAGCCATTTG CTCCTGCACG TGTTTCGGAG GCCAGCGGGG CTGGCGCTGT
    GACAACTGCC GTAGACCTGG GGCTGCTGAA CCCAGTCCCG ATGGCACCAC CGGCCACACC
    TACAACCAGT ATACACAGAG ATACAATCAG AGAACAAACA CTAACGTAAA TTGCCCCATT
    GAGTGCTTCA TGCCGCTAGA TGTGCAAGCT GACAGAGACG ATTCTCGAGA GTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.