Fga cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Fga
Entrez Gene ID	14161
Full Name	fibrinogen alpha chain
Synonyms	Fib
General protein information	Preferred Names fibrinogen alpha chain Names fibrinogen alpha chain fibrinogen, A alpha polypeptide
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 3 Map Location: 3 E3\|3 36.96 cM
Summary	This gene encodes a subunit of the coagulation factor fibrinogen, which is a component of the blood clot. The encoded protein is proteolytically processed by thrombin during the conversion of fibrinogen to fibrin. Mice lacking the encoded protein display bleeding in the peritoneal cavity, skin and soft tissues around joints immediately after birth, and are predisposed to spontaneous fatal abdominal hemorrhage as they grow. Pregnant mice lacking the encoded protein succumb to uterine bleeding during gestation. Alternative splicing results in multiple transcript variants encoding different isoforms that may undergo similar proteolytic processing. [provided by RefSeq, Nov 2015].

mRNA and Protein(s)

mRNA	Protein	Name
NM_010196.4	NP_034326.1	fibrinogen alpha chain isoform 2 precursor
NM_001111048.2	NP_001104518.1	fibrinogen alpha chain isoform 1 preproprotein

Pathways from BioSystems

Homologies

Homo sapiens (human)	FGA	NP_000499.1
	FGA	XP_001088855.2
Canis lupus familiaris (dog)	FGA	XP_532697.2
Rattus norvegicus (Norway rat)	Fga	NP_001008724.1
Danio rerio (zebrafish)	fga	NP_001181918.1
Mus musculus (house mouse)	Fga	NP_001104518.1
Gallus gallus (chicken)	FGA	NP_001258840.1
Pan troglodytes (chimpanzee)	FGA	XP_001136067.1
Xenopus tropicalis (tropical clawed frog)	fga	XP_002933535.2

Gene Ontology
Bibliography

Related articles in PubMed

Plasminogen Deficiency Delays the Onset and Protects from Demyelination and Paralysis in Autoimmune Neuroinflammatory Disease.
Shaw MA, Gao Z, McElhinney KE, Thornton S, Flick MJ, Lane A, Degen JL, Ryu JK, Akassoglou K, Mullins ES
The Journal of neuroscience : the official journal of the Society for Neuroscience37(14)3776-3788(2017 Apr)

Quantitative proteomic profiling of the extracellular matrix of pancreatic islets during the angiogenic switch and insulinoma progression.
Naba A, Clauser KR, Mani DR, Carr SA, Hynes RO
Scientific reports740495(2017 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

cellular fibrin uptake was diminished by elimination of CCR2-expressing cells.
Title: A CCR2 macrophage endocytic pathway mediates extravascular fibrin clearance in vivo.

Mice expressing a mutant form of fibrinogen cannot support fibrin formation exhibit compromised antimicrobial host defense.
Title: Mice expressing a mutant form of fibrinogen that cannot support fibrin formation exhibit compromised antimicrobial host defense.

Data indicate RU-505 as an effective inhibitor of the beta-amyloid (Abeta)-fibrinogen interaction.
Title: A novel A?-fibrinogen interaction inhibitor rescues altered thrombosis and cognitive decline in Alzheimer's disease mice.

These results suggest that immunoreactivity of Fg in the kidney, as well as urinary excretion of Fg, serves as a sensitive and early diagnostic translational biomarker for detection of acute kidney injury.
Title: Fibrinogen excretion in the urine and immunoreactivity in the kidney serves as a translational biomarker for acute kidney injury.

Data indicate that FXIIIa-targeted probe signals showed good overlap with immune-fluorescent fibrin staining images.
Title: In vivo near-infrared imaging of fibrin deposition in thromboembolic stroke in mice.

The following Fga gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Fga cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu05717	NM_010196.4 Latest version!	Mus musculus fibrinogen alpha chain (Fga), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$328.30 $469.00
OMu21188	NM_001111048.2 Latest version!	Mus musculus fibrinogen alpha chain (Fga), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Fga ( NM_010196.4 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_034326.1 Mus musculus fibrinogen alpha chain (Fga), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OMu05717
Clone ID Related Accession (Same CDS sequence)	NM_010196.4
Accession Version	NM_010196.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1674bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-22
Organism	Mus musculus(house mouse)
Product	fibrinogen alpha chain isoform 2 precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BY790061.1, AK146828.1, AC138394.7, BG081027.2 and AW113955.1. On Apr 8, 2015 this sequence version replaced NM_010196.3. Transcript Variant: This variant (2) differs in the 3' UTR and 3' coding region, compared to variant 1. The encoded isoform (2) is shorter and has a distinct C-terminus, compared to isoform 1. This isoform (2) may undergo proteolytic processing similar to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK146828.1, BC005467.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849381, SAMN00849385 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCTGTCCC TGAGGGTCAC CTGCCTCATC TTGAGCGTGG CCAGCACAGT CTGGACTACA 
GATACCGAAG ATAAAGGTGA ATTCCTATCA GAAGGAGGAG GAGTACGTGG CCCAAGAGTT 
GTGGAGAGAC ATCAGAGTCA ATGCAAGGAC TCAGACTGGC CCTTCTGCTC TGATGATGAC 
TGGAACCACA AATGCCCTTC AGGCTGCAGG ATGAAAGGGT TGATTGACGA AGCCAACCAG 
GATTTTACAA ACAGAATCAA CAAGCTCAAA AACTCACTAT TTGACTTTCA AAGGAACAAC 
AAGGATTCTA ACTCACTGAC CAGGAATATC ATGGAGTATT TGAGAGGGGA CTTCGCGAAT 
GCCAACAACT TTGATAACAC TTATGGGCAA GTGTCAGAAG ACCTGAGGCG CAGAATTGAG 
ATCCTGAGGC GAAAAGTCAT AGAGAAAGCG CAACAGATTC AAGCTCTGCA GAGCAATGTC 
CGGGCTCAGT TGATAGACAT GAAGCGCCTG GAGGTGGATA TTGATATCAA GATCCGCTCT 
TGCAAAGGGT CCTGCAGCAG GGCTGTAAAC CGTGAGATAA ATCTACAGGA CTATGAAGGT 
CACCAAAAAC AGCTTCAACA GGTCATCGCT AAAGAATTGC TTCCTACAAA AGACAGGCAG 
TACTTGCCAG CACTAAAAAT GTCTCCAGTT CCCGACTTGG TTCCCGGAAG TTTTAAGAGC 
CAGCTTCAGG AGGCCCCTCC AGAGTGGAAG GCATTAACAG AAATGCGGCA GATGAGAATG 
GAGCTGGAGA GACCTGGGAA GGATGGGGGT TCGCGAGGAG ATTCACCAGG AGACTCGCGG 
GGAGATTCTA GAGGGGACTT TGCAACACGT GGACCAGGGT CAAAGGCAGA AAACCCCACG 
AACCCTGGAC CTGGTGGATC TGGGTATTGG CGTCCTGGGA ACTCCGGGTC TGGAAGTGAT 
GGAAATCGGA ACCCTGGGAC CACGGGGTCT GATGGCACTG GAGACTGGGG TACCGGAAGC 
CCTAGACCTG GCTCAGACTC TGGGAACTTT AGGCCTGCCA ACCCTAACTG GGGTGTGTTT 
TCAGAGTTTG GAGACAGTAG CAGCCCAGCC ACAAGAAAAG AGTATCACAC AGGTAAAGCG 
GTCACTTCTA AAGGAGATAA AGAGCTCCTG ATTGGAAAGG AGAAAGTCAC CTCTTCTGGC 
ACAAGCACCA CACATCGTTC ATGCTCTAAA ACCATTACCA AGACTGTCAC AGGTCCTGAT 
GGTCGCCGAG AAGTGGTCAA AGAAGTGATC ACCTCGGATG ATGGCTCAGA CTGTGGCGAT 
GCCACCGAGT TAGACATATC CCACAGTTTT AGCGGCAGTC TCGACGAACT CTCTGAAAGG 
CATCCTGACC TTTCTGGGTT TTTTGACAAC CACTTTGGTT TAATCTCACC TAACTTCAAA 
GAATTTGGCA GTAAGACCCA TTCTGATTCC GACATCCTCA CAAACATTGA GGACCCCAGC 
TCCCATGTAC CTGAGTTTTC TTCCAGTAGT AAAACCTCAA CTGTCAAAAA ACAAGTAACC 
AAGACCTATA AAATGGCAGA CGAGGCAGGA AGCGAAGCTC ACCGGGAAGG AGAAACTCGC 
AACACTAAGA GGGGCCGTGC CAGAGCTCGC CCAACGAGAG GTATCGACAC TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_034326.1
CDS	63..1736
Translation	MLSLRVTCLILSVASTVWTTDTEDKGEFLSEGGGVRGPRVVERHQSQCKDSDWPFCSDDDWNHKCPSGCRMKGLIDEANQDFTNRINKLKNSLFDFQRNNKDSNSLTRNIMEYLRGDFANANNFDNTYGQVSEDLRRRIEILRRKVIEKAQQIQALQSNVRAQLIDMKRLEVDIDIKIRSCKGSCSRAVNREINLQDYEGHQKQLQQVIAKELLPTKDRQYLPALKMSPVPDLVPGSFKSQLQEAPPEWKALTEMRQMRMELERPGKDGGSRGDSPGDSRGDSRGDFATRGPGSKAENPTNPGPGGSGYWRPGNSGSGSDGNRNPGTTGSDGTGDWGTGSPRPGSDSGNFRPANPNWGVFSEFGDSSSPATRKEYHTGKAVTSKGDKELLIGKEKVTSSGTSTTHRSCSKTITKTVTGPDGRREVVKEVITSDDGSDCGDATELDISHSFSGSLDELSERHPDLSGFFDNHFGLISPNFKEFGSKTHSDSDILTNIEDPSSHVPEFSSSSKTSTVKKQVTKTYKMADEAGSEAHREGETRNTKRGRARARPTRGIDT

Target ORF information:

RefSeq Version	NM_010196.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus fibrinogen alpha chain (Fga), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_010196.4

ORF Insert Sequence:

ATGCTGTCCC TGAGGGTCAC CTGCCTCATC TTGAGCGTGG CCAGCACAGT CTGGACTACA 
GATACCGAAG ATAAAGGTGA ATTCCTATCA GAAGGAGGAG GAGTACGTGG CCCAAGAGTT 
GTGGAGAGAC ATCAGAGTCA ATGCAAGGAC TCAGACTGGC CCTTCTGCTC TGATGATGAC 
TGGAACCACA AATGCCCTTC AGGCTGCAGG ATGAAAGGGT TGATTGACGA AGCCAACCAG 
GATTTTACAA ACAGAATCAA CAAGCTCAAA AACTCACTAT TTGACTTTCA AAGGAACAAC 
AAGGATTCTA ACTCACTGAC CAGGAATATC ATGGAGTATT TGAGAGGGGA CTTCGCGAAT 
GCCAACAACT TTGATAACAC TTATGGGCAA GTGTCAGAAG ACCTGAGGCG CAGAATTGAG 
ATCCTGAGGC GAAAAGTCAT AGAGAAAGCG CAACAGATTC AAGCTCTGCA GAGCAATGTC 
CGGGCTCAGT TGATAGACAT GAAGCGCCTG GAGGTGGATA TTGATATCAA GATCCGCTCT 
TGCAAAGGGT CCTGCAGCAG GGCTGTAAAC CGTGAGATAA ATCTACAGGA CTATGAAGGT 
CACCAAAAAC AGCTTCAACA GGTCATCGCT AAAGAATTGC TTCCTACAAA AGACAGGCAG 
TACTTGCCAG CACTAAAAAT GTCTCCAGTT CCCGACTTGG TTCCCGGAAG TTTTAAGAGC 
CAGCTTCAGG AGGCCCCTCC AGAGTGGAAG GCATTAACAG AAATGCGGCA GATGAGAATG 
GAGCTGGAGA GACCTGGGAA GGATGGGGGT TCGCGAGGAG ATTCACCAGG AGACTCGCGG 
GGAGATTCTA GAGGGGACTT TGCAACACGT GGACCAGGGT CAAAGGCAGA AAACCCCACG 
AACCCTGGAC CTGGTGGATC TGGGTATTGG CGTCCTGGGA ACTCCGGGTC TGGAAGTGAT 
GGAAATCGGA ACCCTGGGAC CACGGGGTCT GATGGCACTG GAGACTGGGG TACCGGAAGC 
CCTAGACCTG GCTCAGACTC TGGGAACTTT AGGCCTGCCA ACCCTAACTG GGGTGTGTTT 
TCAGAGTTTG GAGACAGTAG CAGCCCAGCC ACAAGAAAAG AGTATCACAC AGGTAAAGCG 
GTCACTTCTA AAGGAGATAA AGAGCTCCTG ATTGGAAAGG AGAAAGTCAC CTCTTCTGGC 
ACAAGCACCA CACATCGTTC ATGCTCTAAA ACCATTACCA AGACTGTCAC AGGTCCTGAT 
GGTCGCCGAG AAGTGGTCAA AGAAGTGATC ACCTCGGATG ATGGCTCAGA CTGTGGCGAT 
GCCACCGAGT TAGACATATC CCACAGTTTT AGCGGCAGTC TCGACGAACT CTCTGAAAGG 
CATCCTGACC TTTCTGGGTT TTTTGACAAC CACTTTGGTT TAATCTCACC TAACTTCAAA 
GAATTTGGCA GTAAGACCCA TTCTGATTCC GACATCCTCA CAAACATTGA GGACCCCAGC 
TCCCATGTAC CTGAGTTTTC TTCCAGTAGT AAAACCTCAA CTGTCAAAAA ACAAGTAACC 
AAGACCTATA AAATGGCAGA CGAGGCAGGA AGCGAAGCTC ACCGGGAAGG AGAAACTCGC 
AACACTAAGA GGGGCCGTGC CAGAGCTCGC CCAACGAGAG GTATCGACAC TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Fga ( NM_001111048.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001104518.1 Mus musculus fibrinogen alpha chain (Fga), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OMu21188
Clone ID Related Accession (Same CDS sequence)	NM_001111048.2
Accession Version	NM_001111048.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2370bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-28
Organism	Mus musculus(house mouse)
Product	fibrinogen alpha chain isoform 1 preproprotein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BY790061.1, AK146828.1, W78420.1, AC138394.7, BG081027.2 and AW113955.1. On Apr 8, 2015 this sequence version replaced NM_001111048.1. Transcript Variant: This variant (1) encodes the longer isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMN00849381, SAMN00849385 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCTGTCCC TGAGGGTCAC CTGCCTCATC TTGAGCGTGG CCAGCACAGT CTGGACTACA 
GATACCGAAG ATAAAGGTGA ATTCCTATCA GAAGGAGGAG GAGTACGTGG CCCAAGAGTT 
GTGGAGAGAC ATCAGAGTCA ATGCAAGGAC TCAGACTGGC CCTTCTGCTC TGATGATGAC 
TGGAACCACA AATGCCCTTC AGGCTGCAGG ATGAAAGGGT TGATTGACGA AGCCAACCAG 
GATTTTACAA ACAGAATCAA CAAGCTCAAA AACTCACTAT TTGACTTTCA AAGGAACAAC 
AAGGATTCTA ACTCACTGAC CAGGAATATC ATGGAGTATT TGAGAGGGGA CTTCGCGAAT 
GCCAACAACT TTGATAACAC TTATGGGCAA GTGTCAGAAG ACCTGAGGCG CAGAATTGAG 
ATCCTGAGGC GAAAAGTCAT AGAGAAAGCG CAACAGATTC AAGCTCTGCA GAGCAATGTC 
CGGGCTCAGT TGATAGACAT GAAGCGCCTG GAGGTGGATA TTGATATCAA GATCCGCTCT 
TGCAAAGGGT CCTGCAGCAG GGCTGTAAAC CGTGAGATAA ATCTACAGGA CTATGAAGGT 
CACCAAAAAC AGCTTCAACA GGTCATCGCT AAAGAATTGC TTCCTACAAA AGACAGGCAG 
TACTTGCCAG CACTAAAAAT GTCTCCAGTT CCCGACTTGG TTCCCGGAAG TTTTAAGAGC 
CAGCTTCAGG AGGCCCCTCC AGAGTGGAAG GCATTAACAG AAATGCGGCA GATGAGAATG 
GAGCTGGAGA GACCTGGGAA GGATGGGGGT TCGCGAGGAG ATTCACCAGG AGACTCGCGG 
GGAGATTCTA GAGGGGACTT TGCAACACGT GGACCAGGGT CAAAGGCAGA AAACCCCACG 
AACCCTGGAC CTGGTGGATC TGGGTATTGG CGTCCTGGGA ACTCCGGGTC TGGAAGTGAT 
GGAAATCGGA ACCCTGGGAC CACGGGGTCT GATGGCACTG GAGACTGGGG TACCGGAAGC 
CCTAGACCTG GCTCAGACTC TGGGAACTTT AGGCCTGCCA ACCCTAACTG GGGTGTGTTT 
TCAGAGTTTG GAGACAGTAG CAGCCCAGCC ACAAGAAAAG AGTATCACAC AGGTAAAGCG 
GTCACTTCTA AAGGAGATAA AGAGCTCCTG ATTGGAAAGG AGAAAGTCAC CTCTTCTGGC 
ACAAGCACCA CACATCGTTC ATGCTCTAAA ACCATTACCA AGACTGTCAC AGGTCCTGAT 
GGTCGCCGAG AAGTGGTCAA AGAAGTGATC ACCTCGGATG ATGGCTCAGA CTGTGGCGAT 
GCCACCGAGT TAGACATATC CCACAGTTTT AGCGGCAGTC TCGACGAACT CTCTGAAAGG 
CATCCTGACC TTTCTGGGTT TTTTGACAAC CACTTTGGTT TAATCTCACC TAACTTCAAA 
GAATTTGGCA GTAAGACCCA TTCTGATTCC GACATCCTCA CAAACATTGA GGACCCCAGC 
TCCCATGTAC CTGAGTTTTC TTCCAGTAGT AAAACCTCAA CTGTCAAAAA ACAAGTAACC 
AAGACCTATA AAATGGCAGA CGAGGCAGGA AGCGAAGCTC ACCGGGAAGG AGAAACTCGC 
AACACTAAGA GGGGCCGTGC CAGAGCTCGC CCAACGAGAG ACTGTGATGA TGTCCTCCAA 
ACACAAACTT CAGGTGCCCA AAATGGCATT TTCAGTATCA AGCCACCTGG ATCCAGTAAG 
GTTTTTTCTG TTTATTGCGA TCAAGAGACC AGTTTGGGAG GATGGCTTTT GATCCAGCAA 
AGAATGGATG GATCGCTGAA TTTTAACCGG ACCTGGCAAG ACTACAAGAG AGGTTTCGGC 
AGCCTGAATG ACAAAGGGGA AGGAGAATTC TGGCTAGGCA ATGACTACCT CCACTTACTC 
ACTCTGAGGG GCTCAGTTCT CAGGGTTGAA TTAGAGGACT GGGCTGGAAA AGAGGCTTAT 
GCGGAGTACC ACTTCCGGGT AGGCTCTGAG GCAGAGGGCT ACGCGCTGCA GGTCTCCTCC 
TACCGGGGCA CTGCTGGAGA TGCTCTGGTG CAGGGCTCTG TGGAGGAGGG GACAGAATAC 
ACCTCACACA GCAACATGCA GTTCAGTACC TTTGACAGAG ATGCCGACCA ATGGGAAGAG 
AACTGTGCAG AGGTCTATGG GGGAGGCTGG TGGTACAACA GCTGTCAGGC CGCCAATCTC 
AATGGCATTT ACTACCCCGG GGGCACCTAC GATCCCAGGA ACAACAGCCC CTATGAGATA 
GAGAACGGGG TGGTCTGGGT TCCCTTCAGA GGAGCAGATT ATTCCCTCAG GGCCGTTCGG 
ATGAAAATCA GACCGCTGGT GGGACAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001104518.1
CDS	63..2432
Translation	MLSLRVTCLILSVASTVWTTDTEDKGEFLSEGGGVRGPRVVERHQSQCKDSDWPFCSDDDWNHKCPSGCRMKGLIDEANQDFTNRINKLKNSLFDFQRNNKDSNSLTRNIMEYLRGDFANANNFDNTYGQVSEDLRRRIEILRRKVIEKAQQIQALQSNVRAQLIDMKRLEVDIDIKIRSCKGSCSRAVNREINLQDYEGHQKQLQQVIAKELLPTKDRQYLPALKMSPVPDLVPGSFKSQLQEAPPEWKALTEMRQMRMELERPGKDGGSRGDSPGDSRGDSRGDFATRGPGSKAENPTNPGPGGSGYWRPGNSGSGSDGNRNPGTTGSDGTGDWGTGSPRPGSDSGNFRPANPNWGVFSEFGDSSSPATRKEYHTGKAVTSKGDKELLIGKEKVTSSGTSTTHRSCSKTITKTVTGPDGRREVVKEVITSDDGSDCGDATELDISHSFSGSLDELSERHPDLSGFFDNHFGLISPNFKEFGSKTHSDSDILTNIEDPSSHVPEFSSSSKTSTVKKQVTKTYKMADEAGSEAHREGETRNTKRGRARARPTRDCDDVLQTQTSGAQNGIFSIKPPGSSKVFSVYCDQETSLGGWLLIQQRMDGSLNFNRTWQDYKRGFGSLNDKGEGEFWLGNDYLHLLTLRGSVLRVELEDWAGKEAYAEYHFRVGSEAEGYALQVSSYRGTAGDALVQGSVEEGTEYTSHSNMQFSTFDRDADQWEENCAEVYGGGWWYNSCQAANLNGIYYPGGTYDPRNNSPYEIENGVVWVPFRGADYSLRAVRMKIRPLVGQ

Target ORF information:

RefSeq Version	NM_001111048.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus fibrinogen alpha chain (Fga), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001111048.2

ORF Insert Sequence:

ATGCTGTCCC TGAGGGTCAC CTGCCTCATC TTGAGCGTGG CCAGCACAGT CTGGACTACA 
GATACCGAAG ATAAAGGTGA ATTCCTATCA GAAGGAGGAG GAGTACGTGG CCCAAGAGTT 
GTGGAGAGAC ATCAGAGTCA ATGCAAGGAC TCAGACTGGC CCTTCTGCTC TGATGATGAC 
TGGAACCACA AATGCCCTTC AGGCTGCAGG ATGAAAGGGT TGATTGACGA AGCCAACCAG 
GATTTTACAA ACAGAATCAA CAAGCTCAAA AACTCACTAT TTGACTTTCA AAGGAACAAC 
AAGGATTCTA ACTCACTGAC CAGGAATATC ATGGAGTATT TGAGAGGGGA CTTCGCGAAT 
GCCAACAACT TTGATAACAC TTATGGGCAA GTGTCAGAAG ACCTGAGGCG CAGAATTGAG 
ATCCTGAGGC GAAAAGTCAT AGAGAAAGCG CAACAGATTC AAGCTCTGCA GAGCAATGTC 
CGGGCTCAGT TGATAGACAT GAAGCGCCTG GAGGTGGATA TTGATATCAA GATCCGCTCT 
TGCAAAGGGT CCTGCAGCAG GGCTGTAAAC CGTGAGATAA ATCTACAGGA CTATGAAGGT 
CACCAAAAAC AGCTTCAACA GGTCATCGCT AAAGAATTGC TTCCTACAAA AGACAGGCAG 
TACTTGCCAG CACTAAAAAT GTCTCCAGTT CCCGACTTGG TTCCCGGAAG TTTTAAGAGC 
CAGCTTCAGG AGGCCCCTCC AGAGTGGAAG GCATTAACAG AAATGCGGCA GATGAGAATG 
GAGCTGGAGA GACCTGGGAA GGATGGGGGT TCGCGAGGAG ATTCACCAGG AGACTCGCGG 
GGAGATTCTA GAGGGGACTT TGCAACACGT GGACCAGGGT CAAAGGCAGA AAACCCCACG 
AACCCTGGAC CTGGTGGATC TGGGTATTGG CGTCCTGGGA ACTCCGGGTC TGGAAGTGAT 
GGAAATCGGA ACCCTGGGAC CACGGGGTCT GATGGCACTG GAGACTGGGG TACCGGAAGC 
CCTAGACCTG GCTCAGACTC TGGGAACTTT AGGCCTGCCA ACCCTAACTG GGGTGTGTTT 
TCAGAGTTTG GAGACAGTAG CAGCCCAGCC ACAAGAAAAG AGTATCACAC AGGTAAAGCG 
GTCACTTCTA AAGGAGATAA AGAGCTCCTG ATTGGAAAGG AGAAAGTCAC CTCTTCTGGC 
ACAAGCACCA CACATCGTTC ATGCTCTAAA ACCATTACCA AGACTGTCAC AGGTCCTGAT 
GGTCGCCGAG AAGTGGTCAA AGAAGTGATC ACCTCGGATG ATGGCTCAGA CTGTGGCGAT 
GCCACCGAGT TAGACATATC CCACAGTTTT AGCGGCAGTC TCGACGAACT CTCTGAAAGG 
CATCCTGACC TTTCTGGGTT TTTTGACAAC CACTTTGGTT TAATCTCACC TAACTTCAAA 
GAATTTGGCA GTAAGACCCA TTCTGATTCC GACATCCTCA CAAACATTGA GGACCCCAGC 
TCCCATGTAC CTGAGTTTTC TTCCAGTAGT AAAACCTCAA CTGTCAAAAA ACAAGTAACC 
AAGACCTATA AAATGGCAGA CGAGGCAGGA AGCGAAGCTC ACCGGGAAGG AGAAACTCGC 
AACACTAAGA GGGGCCGTGC CAGAGCTCGC CCAACGAGAG ACTGTGATGA TGTCCTCCAA 
ACACAAACTT CAGGTGCCCA AAATGGCATT TTCAGTATCA AGCCACCTGG ATCCAGTAAG 
GTTTTTTCTG TTTATTGCGA TCAAGAGACC AGTTTGGGAG GATGGCTTTT GATCCAGCAA 
AGAATGGATG GATCGCTGAA TTTTAACCGG ACCTGGCAAG ACTACAAGAG AGGTTTCGGC 
AGCCTGAATG ACAAAGGGGA AGGAGAATTC TGGCTAGGCA ATGACTACCT CCACTTACTC 
ACTCTGAGGG GCTCAGTTCT CAGGGTTGAA TTAGAGGACT GGGCTGGAAA AGAGGCTTAT 
GCGGAGTACC ACTTCCGGGT AGGCTCTGAG GCAGAGGGCT ACGCGCTGCA GGTCTCCTCC 
TACCGGGGCA CTGCTGGAGA TGCTCTGGTG CAGGGCTCTG TGGAGGAGGG GACAGAATAC 
ACCTCACACA GCAACATGCA GTTCAGTACC TTTGACAGAG ATGCCGACCA ATGGGAAGAG 
AACTGTGCAG AGGTCTATGG GGGAGGCTGG TGGTACAACA GCTGTCAGGC CGCCAATCTC 
AATGGCATTT ACTACCCCGG GGGCACCTAC GATCCCAGGA ACAACAGCCC CTATGAGATA 
GAGAACGGGG TGGTCTGGGT TCCCTTCAGA GGAGCAGATT ATTCCCTCAG GGCCGTTCGG 
ATGAAAATCA GACCGCTGGT GGGACAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Plasminogen Deficiency Delays the Onset and Protects from Demyelination and Paralysis in Autoimmune Neuroinflammatory Disease.
The Journal of neuroscience : the official journal of the Society for Neuroscience37(14)3776-3788(2017 Apr)
Shaw MA,Gao Z,McElhinney KE,Thornton S,Flick MJ,Lane A,Degen JL,Ryu JK,Akassoglou K,Mullins ES

Quantitative proteomic profiling of the extracellular matrix of pancreatic islets during the angiogenic switch and insulinoma progression.
Scientific reports740495(2017 Jan)
Naba A,Clauser KR,Mani DR,Carr SA,Hynes RO

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Fga cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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