Fdx1 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Fdx1
Entrez Gene ID	14148
Full Name	ferredoxin 1
General protein information	Preferred Names ferredoxin 1 Names adrenodoxin, mitochondrial adrenal ferredoxin
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 9 Map Location: 9\|9 A5.3
Summary	Ferrodoxins are iron-sulfur proteins that facilitate monooxygenase reactions catalyzed by P450 enzymes. The protein encoded by this gene is present in the mitochondrial matrix and transfers electrons from ferredoxin reductase to steroidogenic mitochondrial cytochrome P450 proteins. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Sep 2014].

mRNA and Protein(s)

mRNA	Protein	Name
NM_001301728.1	NP_001288657.1	adrenodoxin, mitochondrial isoform 2 precursor
NM_007996.2	NP_032022.1	adrenodoxin, mitochondrial isoform 1 precursor

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	FDX1	XP_508877.3
	LOC709110	XP_001105034.2
Canis lupus familiaris (dog)	FDX1	XP_005620366.1
Bos taurus (cattle)	FDX1	NP_851354.1
Rattus norvegicus (Norway rat)	Fdx1	NP_058822.1
Danio rerio (zebrafish)	fdx1	XP_001922722.2
Drosophila melanogaster (fruit fly)	CG1319	NP_647889.2
Gallus gallus (chicken)	FDX1	NP_001185597.1
Xenopus tropicalis (tropical clawed frog)	fdx1	XP_002938002.1
Mus musculus (house mouse)	Fdx1	NP_032022.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP003212	XP_312921.3
Homo sapiens (human)	FDX1	NP_004100.1

Gene Ontology
Bibliography

Related articles in PubMed

Transcription factors SF1 and cJUN cooperate to activate the Fdx1 promoter in MA-10 Leydig cells.
Roumaud P, Rwigemera A, Martin LJ
The Journal of steroid biochemistry and molecular biology171121-132(2017 07)

High-throughput discovery of novel developmental phenotypes.
Dickinson ME, Flenniken AM, Ji X, Teboul L, Wong MD, White JK, Meehan TF, Weninger WJ, Westerberg H, Adissu H, Baker CN, Bower L, Brown JM, Caddle LB, Chiani F, Clary D, Cleak J, Daly MJ, Denegre JM, Doe B, Dolan ME, Edie SM, Fuchs H, Gailus-Durner V, Galli A, Gambadoro A, Gallegos J, Guo S, Horner NR, Hsu CW, Johnson SJ, Kalaga S, Keith LC, Lanoue L, Lawson TN, Lek M, Mark M, Marschall S, Mason J, McElwee ML, Newbigging S, Nutter LM, Peterson KA, Ramirez-Solis R, Rowland DJ, Ryder E, Samocha KE, Seavitt JR, Selloum M, Szoke-Kovacs Z, Tamura M, Trainor AG, Tudose I, Wakana S, Warren J, Wendling O, West DB, Wong L, Yoshiki A, , , , , , , , , MacArthur DG, Tocchini-Valentini GP, Gao X, Flicek P, Bradley A, Skarnes WC, Justice MJ, Parkinson HE, Moore M, Wells S, Braun RE, Svenson KL, de Angelis MH, Herault Y, Mohun T, Mallon AM, Henkelman RM, Brown SD, Adams DJ, Lloyd KC, McKerlie C, Beaudet AL, Bu?an M, Murray SA
Nature537(7621)508-514(2016 09)

Role of protein kinase C-mediated protein phosphorylation in mitochondrial translocation of mouse CYP1A1, which contains a non-canonical targeting signal.
Dasari VR, Anandatheerthavarada HK, Robin MA, Boopathi E, Biswas G, Fang JK, Nebert DW, Avadhani NG
The Journal of biological chemistry281(41)30834-47(2006 Oct)

Preimplantation mouse blastocysts fail to express CYP genes required for estrogen biosynthesis.
Str?mstedt M, Keeney DS, Waterman MR, Paria BC, Conley AJ, Dey SK
Molecular reproduction and development43(4)428-36(1996 Apr)

A full-length cDNA encoding mouse adrenodoxin.
Stromstedt M, Waterman MR
Biochimica et biophysica acta1261(1)126-8(1995 Mar)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Data suggest that Sf1 and c-jun interact and cooperate to activate the Fdx1 promoter in MA-10 (tumorigenic cell line) and TM3 (non-tumorigenic cell line) Leydig cells; such activation requires different regulatory elements located between -124 and -306 bp of Fdx1 promoter and involves recruitment of Sf1 to this region. (Sf1 = splicing factor 1; c-jun = proto-oncogene c-jun; Fdx1 = ferredoxin 1)
Title: Transcription factors SF1 and cJUN cooperate to activate the Fdx1 promoter in MA-10 Leydig cells.

mouse MT-CYP1A1 is an extrinsic membrane protein, which exhibited high FDX1 plus FDXR-mediated N-demethylation of a number of tricyclic antidepressants, pain killers, anti-psychotics, and narcotics that are poor substrates for microsomal CYP1A1
Title: Role of protein kinase C-mediated protein phosphorylation in mitochondrial translocation of mouse CYP1A1, which contains a non-canonical targeting signal.

The following Fdx1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Fdx1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu14468	NM_007996.2 Latest version!	Mus musculus ferredoxin 1 (Fdx1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00
OMu67725	NM_001301728.1 Latest version!	Mus musculus ferredoxin 1 (Fdx1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Fdx1 ( NM_007996.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_032022.1 Mus musculus ferredoxin 1 (Fdx1), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OMu14468
Clone ID Related Accession (Same CDS sequence)	NM_007996.2
Accession Version	NM_007996.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 567bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-20
Organism	Mus musculus(house mouse)
Product	adrenodoxin, mitochondrial isoform 1 precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BB578768.1, D43689.1 and AI050584.1. On Sep 11, 2014 this sequence version replaced NM_007996.1. Transcript Variant: This variant (1) represents the shorter transcript and encodes the longer isoform (1). ##Evidence-Data-START## Transcript exon combination :: CD768318.1, L29123.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849386 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGCGGCCG CTCCGGGCGC CCGACTCCTG CGCGCGGCCT GCGCCTCCGT CCCTTTCCGC 
GGCCTTGACC GCTGTCGGCT GCTGGTCTGC GGGACCGGAG CGGGAACTGC CATCTCTCCG 
TGGACCCCGA GTCCCCGCCT GCATGCAGAG GCCGGGCCCG GCCGGCCGCT GAGCGTGTCT 
GCGCGCGCGC GGAGCAGCTC AGAAGATAAG ATAACAGTCC ACTTCAAGAA CCGAGATGGC 
GAGACGCTAA CGACCAAGGG GAAAATTGGC GACTCTCTGC TAGATGTTGT GATTGAGAAC 
AACTTAGATA TCGATGGGTT TGGTGCGTGT GAGGGAACGT TGGCTTGCTC TACTTGTCAT 
CTTATCTTTG AGGATCACAT CTATGAGAAG TTAGATGCCA TTACTGATGA AGAGAATGAC 
ATGCTTGACC TGGCTTTTGG ACTAACAGAC AGGTCAAGGT TGGGCTGCCA AGTTTGTCTG 
ACCAAGGCTA TGGACAATAT GACTGTGCGT GTGCCTGAAG CAGTGGCGGA TGTCCGACAG 
TCTGTTGACA TGAGCAAGAA TTCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_032022.1
CDS	136..702
Translation	MAAAPGARLLRAACASVPFRGLDRCRLLVCGTGAGTAISPWTPSPRLHAEAGPGRPLSVSARARSSSEDKITVHFKNRDGETLTTKGKIGDSLLDVVIENNLDIDGFGACEGTLACSTCHLIFEDHIYEKLDAITDEENDMLDLAFGLTDRSRLGCQVCLTKAMDNMTVRVPEAVADVRQSVDMSKNS

Target ORF information:

RefSeq Version	NM_007996.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus ferredoxin 1 (Fdx1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_007996.2

ORF Insert Sequence:

ATGGCGGCCG CTCCGGGCGC CCGACTCCTG CGCGCGGCCT GCGCCTCCGT CCCTTTCCGC 
GGCCTTGACC GCTGTCGGCT GCTGGTCTGC GGGACCGGAG CGGGAACTGC CATCTCTCCG 
TGGACCCCGA GTCCCCGCCT GCATGCAGAG GCCGGGCCCG GCCGGCCGCT GAGCGTGTCT 
GCGCGCGCGC GGAGCAGCTC AGAAGATAAG ATAACAGTCC ACTTCAAGAA CCGAGATGGC 
GAGACGCTAA CGACCAAGGG GAAAATTGGC GACTCTCTGC TAGATGTTGT GATTGAGAAC 
AACTTAGATA TCGATGGGTT TGGTGCGTGT GAGGGAACGT TGGCTTGCTC TACTTGTCAT 
CTTATCTTTG AGGATCACAT CTATGAGAAG TTAGATGCCA TTACTGATGA AGAGAATGAC 
ATGCTTGACC TGGCTTTTGG ACTAACAGAC AGGTCAAGGT TGGGCTGCCA AGTTTGTCTG 
ACCAAGGCTA TGGACAATAT GACTGTGCGT GTGCCTGAAG CAGTGGCGGA TGTCCGACAG 
TCTGTTGACA TGAGCAAGAA TTCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Fdx1 ( NM_001301728.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001288657.1 Mus musculus ferredoxin 1 (Fdx1), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OMu67725
Clone ID Related Accession (Same CDS sequence)	NM_001301728.1
Accession Version	NM_001301728.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 501bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-24
Organism	Mus musculus(house mouse)
Product	adrenodoxin, mitochondrial isoform 2 precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BB578768.1, D43689.1, AK148115.1, AC140363.3 and BX516353.1. On Sep 11, 2014 this sequence version replaced XM_006510006.1. Transcript Variant: This variant (2) contains an alternate exon, which results in a frameshift, compared to variant 1. The resulting protein (isoform 2) has a shorter, distinct C-terminus compared to isoform 1. ##Evidence-Data-START## Transcript exon combination :: AK148115.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849386 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGCGGCCG CTCCGGGCGC CCGACTCCTG CGCGCGGCCT GCGCCTCCGT CCCTTTCCGC 
GGCCTTGACC GCTGTCGGCT GCTGGTCTGC GGGACCGGAG CGGGAACTGC CATCTCTCCG 
TGGACCCCGA GTCCCCGCCT GCATGCAGAG GCCGGGCCCG GCCGGCCGCT GAGCGTGTCT 
GCGCGCGCGC GGAGCAGCTC AGAAGATAAG ATAACAGTCC ACTTCAAGAA CCGAGATGGC 
GAGACGCTAA CGACCAAGGG GAAAATTGGC GACTCTCTGC TAGATGTTGT GATTGAGAAC 
AACTTAGATA TCGATGGGTT TGGTGCGTGT GAGGGAACGT TGGCTTGCTC TACTTGTCAT 
CTTATCTTTG AGGATCACAT CTATGAGAAG TTAGATGCCA TTACTGATGA AGAGAATGAC 
ATGCTTGACC TGGCTTTTGG ACTAACAGAC AGGAACCTGG AAGACCCACC TATTTCCTTC 
AGCCCAGCAA TTGGTCCCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001288657.1
CDS	136..636
Translation	MAAAPGARLLRAACASVPFRGLDRCRLLVCGTGAGTAISPWTPSPRLHAEAGPGRPLSVSARARSSSEDKITVHFKNRDGETLTTKGKIGDSLLDVVIENNLDIDGFGACEGTLACSTCHLIFEDHIYEKLDAITDEENDMLDLAFGLTDRNLEDPPISFSPAIGP

Target ORF information:

RefSeq Version	NM_001301728.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus ferredoxin 1 (Fdx1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001301728.1

ORF Insert Sequence:

ATGGCGGCCG CTCCGGGCGC CCGACTCCTG CGCGCGGCCT GCGCCTCCGT CCCTTTCCGC 
GGCCTTGACC GCTGTCGGCT GCTGGTCTGC GGGACCGGAG CGGGAACTGC CATCTCTCCG 
TGGACCCCGA GTCCCCGCCT GCATGCAGAG GCCGGGCCCG GCCGGCCGCT GAGCGTGTCT 
GCGCGCGCGC GGAGCAGCTC AGAAGATAAG ATAACAGTCC ACTTCAAGAA CCGAGATGGC 
GAGACGCTAA CGACCAAGGG GAAAATTGGC GACTCTCTGC TAGATGTTGT GATTGAGAAC 
AACTTAGATA TCGATGGGTT TGGTGCGTGT GAGGGAACGT TGGCTTGCTC TACTTGTCAT 
CTTATCTTTG AGGATCACAT CTATGAGAAG TTAGATGCCA TTACTGATGA AGAGAATGAC 
ATGCTTGACC TGGCTTTTGG ACTAACAGAC AGGAACCTGG AAGACCCACC TATTTCCTTC 
AGCCCAGCAA TTGGTCCCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Transcription factors SF1 and cJUN cooperate to activate the Fdx1 promoter in MA-10 Leydig cells.
The Journal of steroid biochemistry and molecular biology171121-132(2017 07)
Roumaud P,Rwigemera A,Martin LJ

High-throughput discovery of novel developmental phenotypes.
Nature537(7621)508-514(2016 09)
Dickinson ME,Flenniken AM,Ji X,Teboul L,Wong MD,White JK,Meehan TF,Weninger WJ,Westerberg H,Adissu H,Baker CN,Bower L,Brown JM,Caddle LB,Chiani F,Clary D,Cleak J,Daly MJ,Denegre JM,Doe B,Dolan ME,Edie SM,Fuchs H,Gailus-Durner V,Galli A,Gambadoro A,Gallegos J,Guo S,Horner NR,Hsu CW,Johnson SJ,Kalaga S,Keith LC,Lanoue L,Lawson TN,Lek M,Mark M,Marschall S,Mason J,McElwee ML,Newbigging S,Nutter LM,Peterson KA,Ramirez-Solis R,Rowland DJ,Ryder E,Samocha KE,Seavitt JR,Selloum M,Szoke-Kovacs Z,Tamura M,Trainor AG,Tudose I,Wakana S,Warren J,Wendling O,West DB,Wong L,Yoshiki A,,,,,,,,,MacArthur DG,Tocchini-Valentini GP,Gao X,Flicek P,Bradley A,Skarnes WC,Justice MJ,Parkinson HE,Moore M,Wells S,Braun RE,Svenson KL,de Angelis MH,Herault Y,Mohun T,Mallon AM,Henkelman RM,Brown SD,Adams DJ,Lloyd KC,McKerlie C,Beaudet AL,Bu?an M,Murray SA

Role of protein kinase C-mediated protein phosphorylation in mitochondrial translocation of mouse CYP1A1, which contains a non-canonical targeting signal.
The Journal of biological chemistry281(41)30834-47(2006 Oct)
Dasari VR,Anandatheerthavarada HK,Robin MA,Boopathi E,Biswas G,Fang JK,Nebert DW,Avadhani NG

Preimplantation mouse blastocysts fail to express CYP genes required for estrogen biosynthesis.
Molecular reproduction and development43(4)428-36(1996 Apr)
Str?mstedt M,Keeney DS,Waterman MR,Paria BC,Conley AJ,Dey SK

A full-length cDNA encoding mouse adrenodoxin.
Biochimica et biophysica acta1261(1)126-8(1995 Mar)
Stromstedt M,Waterman MR

- Most Popular Services
  - 人工遺伝子合成& DNA合成-納期保証
- Services & Products

見積依頼

Eメール

japanmarket@genscript.com

電話

03-6811-6572

FAX

03-6811-6573
050-3174-7589(受注専用)

オンラインフォーム

オンライン注文

Fdx1 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Most Popular Services

Services & Products

見積依頼

Eメール

電話

FAX

オンラインフォーム

サポート

会社情報

サポート

会社情報