F8 cDNA ORF clone, Mus musculus(house mouse)

The following F8 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the F8 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OMu56128 XM_006527789.4
Latest version!
Mus musculus coagulation factor VIII (F8), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1091.30
$1559.00
OMu56129 XM_006527790.4
Latest version!
Mus musculus coagulation factor VIII (F8), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1091.30
$1559.00
OMu56130 XM_006527792.4
Latest version!
Mus musculus coagulation factor VIII (F8), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1091.30
$1559.00
OMu56130 XM_006527791.4
Latest version!
Mus musculus coagulation factor VIII (F8), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1091.30
$1559.00
OMu01160 NM_007977.2
Latest version!
Mus musculus coagulation factor VIII (F8), transcript variant 1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1091.30
$1559.00
OMu08135 NM_001161373.1
Latest version!
Mus musculus coagulation factor VIII (F8), transcript variant 2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1658.30
$2369.00
OMu08137 NM_001161374.1
Latest version!
Mus musculus coagulation factor VIII (F8), transcript variant 3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1091.30
$1559.00
View Old Accession Versions >>
OMu56129 XM_006527790.3 Mus musculus coagulation factor VIII (F8), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1091.30
$1559.00
OMu56130 XM_006527791.3 Mus musculus coagulation factor VIII (F8), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1091.30
$1559.00
OMu56130 XM_006527792.3 Mus musculus coagulation factor VIII (F8), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1091.30
$1559.00
OMu56128 XM_006527789.3 Mus musculus coagulation factor VIII (F8), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1091.30
$1559.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OMu56128
    Clone ID Related Accession (Same CDS sequence) XM_006527789.3 , XM_006527789.4
    Accession Version XM_006527789.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6954bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product coagulation factor VIII isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000086.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006527789.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    ATGCAAATAG CACTCTTCGC TTGCTTCTTT CTGAGCCTTT TCAATTTCTG CTCTAGTGCC 
    ATCAGAAGAT ACTACCTTGG TGCAGTGGAA TTGTCCTGGA ACTATATTCA GAGTGATCTG
    CTCAGTGTGC TGCATACAGA CTCAAGATTT CTTCCTAGAA TGTCAACATC TTTTCCATTC
    AACACCTCCA TCATGTATAA AAAGACTGTG TTTGTAGAGT ACAAGGACCA GCTTTTCAAC
    ATTGCCAAGC CCAGGCCACC CTGGATGGGT TTGCTAGGTC CTACCATTTG GACTGAGGTT
    CATGACACAG TGGTCATTAC ACTTAAAAAC ATGGCTTCTC ATCCTGTCAG TCTTCATGCT
    GTTGGTGTGT CCTACTGGAA AGCTTCTGAG GGAGATGAAT ATGAAGATCA GACAAGCCAA
    ATGGAGAAGG AAGATGATAA AGTTTTCCCT GGTGAAAGTC ATACTTATGT TTGGCAAGTC
    CTGAAAGAGA ATGGTCCAAT GGCCTCTGAC CCTCCATGTC TCACTTACTC ATATATGTCT
    CATGTGGATC TGGTGAAAGA TTTGAATTCA GGCCTCATTG GAGCTCTGCT AGTATGTAAA
    GAAGGCAGTC TCTCCAAAGA AAGAACACAG ATGTTGTACC AATTTGTACT GCTTTTTGCT
    GTATTTGATG AAGGGAAGAG CTGGCACTCA GAAACAAACG ACTCTTATAC ACAGTCTATG
    GATTCTGCAT CTGCTAGAGA CTGGCCTAAA ATGCACACAG TCAATGGCTA TGTAAACAGG
    TCTCTTCCAG GTCTGATTGG ATGCCATAGG AAATCAGTCT ACTGGCACGT GATTGGAATG
    GGCACCACTC CTGAAATACA CTCAATATTC CTCGAAGGTC ACACATTTTT TGTGAGGAAC
    CACCGTCAAG CTTCATTGGA GATATCACCA ATAACTTTCC TTACTGCTCA AACACTCTTG
    ATAGATCTTG GGCAGTTCCT ACTATTTTGT CATATCTCTT CCCATAAACA TGATGGCATG
    GAAGCTTATG TCAAAGTAGA TAGCTGCCCT GAGGAATCCC AATGGCAAAA GAAAAATAAT
    AATGAGGAAA TGGAAGATTA TGATGATGAT CTTTATTCAG AAATGGATAT GTTCACATTG
    GATTATGACA GCTCTCCTTT TATCCAAATT CGCTCGGTTG CTAAAAAGTA CCCTAAAACT
    TGGATACATT ATATTTCTGC TGAGGAGGAA GACTGGGACT ATGCACCTTC AGTTCCTACC
    TCGGATAATG GAAGTTATAA AAGCCAGTAT CTGAGCAATG GTCCTCATCG GATTGGTAGG
    AAATATAAAA AAGTCAGATT TATAGCATAC ACAGATGAAA CCTTTAAGAC TCGTGAAACT
    ATTCAGCATG AATCAGGACT CTTGGGACCT TTACTTTATG GAGAAGTTGG AGACACACTG
    TTGATTATTT TTAAGAATCA AGCAAGCCGA CCATATAACA TTTACCCTCA TGGAATCACT
    GATGTCAGTC CTCTACATGC AAGGAGATTG CCAAGAGGTA TAAAGCACGT GAAGGATTTG
    CCAATTCATC CAGGAGAGAT ATTCAAGTAC AAGTGGACAG TTACAGTAGA AGATGGACCA
    ACTAAATCAG ATCCACGGTG CCTGACCCGC TATTATTCAA GTTTCATTAA CCCTGAGAGA
    GATCTAGCTT CAGGACTGAT TGGCCCTCTT CTCATCTGCT ACAAAGAATC TGTAGATCAA
    AGGGGAAACC AGATGATGTC AGACAAAAGA AATGTCATCC TGTTTTCTAT ATTTGATGAG
    AACCAAAGCT GGTACATCAC AGAGAACATG CAACGCTTCC TCCCCAATGC AGCTAAAACA
    CAGCCCCAGG ACCCTGGGTT CCAGGCCTCC AACATCATGC ACAGCATCAA TGGCTATGTT
    TTTGATAGCT TGGAGTTGAC AGTTTGTTTG CATGAGGTGG CATACTGGCA CATTCTCAGT
    GTTGGAGCAC AGACAGACTT CTTATCTATC TTCTTCTCTG GATATACTTT CAAACACAAA
    ATGGTCTATG AAGATACACT TACCCTGTTC CCATTCTCAG GAGAAACTGT CTTTATGTCG
    ATGGAAAACC CAGGTCTATG GGTCTTGGGG TGTCATAATT CAGACTTTCG GAAGAGAGGT
    ATGACAGCAT TGCTGAAAGT TTCTAGTTGT GACAAGAGCA CTAGTGATTA TTATGAAGAA
    ATATATGAAG ATATTCCAAC ACAGTTGGTG AATGAGAACA ATGTCATTGA TCCCAGAAGC
    TTCTTCCAGA ATACAAATCA TCCTAATACT AGGAAAAAGA AATTCAAAGA TTCCACAATT
    CCAAAAAATG ATATGGAGAA GATTGAGCCT CAGTTTGAAG AGATAGCAGA GATGCTTAAA
    GTACAGAGTG TCTCAGTTAG TGACATGTTG ATGCTCTTGG GACAGAGTCA TCCTACTCCA
    CATGGCTTAT TTTTATCAGA TGGCCAAGAA GCCATCTATG AGGCTATTCA TGATGATCAT
    TCACCAAATG CAATAGACAG CAATGAAGGC CCATCTAAAG TGACCCAACT CAGGCCAGAA
    TCCCATCACA GTGAGAAAAT AGTATTTACT CCTCAGCCCG GCCTCCAGTT AAGATCCAAT
    AAAAGTTTGG AGACAACTAT AGAAGTAAAG TGGAAGAAAC TTGGTTTGCA AGTTTCTAGT
    TTGCCAAGTA ATCTAATGAC TACAACAATT CTGTCAGACA ATTTGAAAGC AACTTTTGAA
    AAGACAGATT CTTCAGGATT TCCAGATATG CCAGTTCACT CTAGTAGTAA ATTAAGTACT
    ACTGCATTTG GTAAGAAAGC ATATTCCCTT GTTGGGTCTC ATGTACCTTT AAACGTGAGT
    GAAGAAAATA GTGATTCCAA CATATTGGAT TCAACTTTAA TGTATAGTCA AGAAAGTTTA
    CCAAGAGATA ATATATTATC AATGGAGAAT GATAGATTAC TCAGAGAGAA GAGGTTTCAT
    GGAATTGCTT TATTGACCAA AGATAATACT TTATTCAAAG ACAATGTCTC CTTAATGAAA
    ACAAACAAAA CATATAATCA TTCAACAACT AATGAAAAAC TACACACTGA GAGCCCAACA
    TCAATTGAGA ATAGTACAAC AGACTTGCAA GATGCCATAT TAAAGGTCAA TAGTGAGATT
    CAAGAAGTAA CAGCTTTGAT TCATGATGGA ACACTTTTAG GCAAAAATTC TACATATTTG
    AGACTAAACC ATATGCTAAA TAGAACTACC TCAACAAAAA ATAAAGACAT ATTTCATAGA
    AAAGATGAAG ATCCTATTCC ACAAGATGAA GAGAATACAA TCATGCCATT TTCCAAGATG
    TTGTTCTTGT CAGAATCTTC AAATTGGTTT AAAAAGACCA ATGGAAATAA TTCCTTGAAC
    TCTGAGCAAG AACATAGTCC AAAGCAATTA GTATATTTAA TGTTTAAAAA ATATGTAAAA
    AATCAAAGTT TCTTGTCAGA GAAAAATAAA GTCACAGTAG AACAGGATGG ATTTACAAAG
    AACATAGGAC TTAAAGACAT GGCTTTTCCA CATAATATGA GCATATTTCT TACCACTTTG
    TCTAACGTAC ATGAAAATGG TAGGCACAAT CAAGAAAAAA ATATTCAGGA AGAGATAGAG
    AAGGAAGCAC TAATTGAAGA GAAAGTAGTT TTGCCCCAGG TGCACGAAGC AACTGGCTCT
    AAGAATTTCT TGAAAGACAT ATTGATACTA GGCACTAGGC AAAATATAAG TTTATATGAA
    GTACATGTAC CAGTACTTCA AAACATCACA TCAATAAACA ATTCAACAAA TACAGTACAG
    ATTCACATGG AGCATTTCTT TAAAAGAAGG AAGGACAAGG AAACAAATTC AGAAGGCTTG
    GTAAATAAAA CCAGAGAAAT GGTAAAAAAC TATCCAAGCC AGAAGAATAT TACTACTCAA
    CGTAGTAAAC GGGCTTTGGG ACAATTCAGA CTGTCAACTC AATGGCTTAA AACCATAAAC
    TGTTCAACAC AGTGTATCAT TAAACAGATA GACCACAGCA AGGAAATGAA AAAGTTCATT
    ACTAAATCTT CCTTATCAGA TTCTTCTGTG ATTAAAAGCA CCACTCAGAC AAATAGTTCT
    GACTCACACA TTGTAAAAAC ATCAGCATTT CCACCAATAG ATCTCAAAAG GAGTCCATTC
    CAAAACAAAT TTTCTCATGT TCAAGCATCA TCCTACATTT ATGACTTTAA GACAAAAAGT
    TCAAGAATTC AAGAAAGCAA TAATTTCTTA AAAGAAACCA AAATAAATAA CCCTTCTTTA
    GCCATTCTAC CATGGAATAT GTTCATAGAT CAAGGAAAAT TTACCTCCCC AGGGAAAAGT
    AACACAAACT CAGTCACATA TAAGAAACGT GAGAACATTA TTTTCTTGAA ACCAACTTTG
    CCTGAAGAAT CTGGCAAAAT TGAATTGCTT CCTCAAGTTT CCATTCAAGA GGAAGAAATT
    TTACCTACAG AAACTAGCCA TGGATCTCCT GGACACTTGA ATCTCATGAA AGAGGTCTTT
    CTTCAGAAAA TACAGGGGCC TACTAAATGG AATAAAGCAA AGAGGCATGG AGAAAGTATA
    AAAGGTAAAA CAGAGAGCTC TAAAAATACT CGCTCAAAAC TGCTAAATCA TCATGCTTGG
    GATTATCATT ATGCTGCACA GATACCAAAA GATATGTGGA AATCCAAAGA GAAGTCACCA
    GAAATTATAT CCATTAAGCA AGAGGACACC ATTTTGTCTC TGAGGCCTCA TGGAAACAGT
    CATTCAATAG GGGCAAATGA GAAACAAAAT TGGCCTCAAA GAGAAACCAC TTGGGTAAAG
    CAAGGCCAAA CTCAAAGGAC ATGCTCTCAA ATCCCACCAG TGTTGAAACG ACATCAAAGG
    GAACTTAGTG CTTTTCAATC AGAACAAGAA GCAACTGACT ATGATGATGC CATCACCATT
    GAAACAATCG AGGATTTTGA CATTTACAGT GAGGACATAA AGCAAGGTCC CCGCAGCTTT
    CAACAGAAAA CAAGGCACTA TTTTATTGCA GCTGTGGAAC GACTCTGGGA CTATGGGATG
    AGTACATCTC ATGTTCTACG AAATAGGTAT CAAAGTGACA ATGTACCTCA GTTCAAGAAA
    GTAGTTTTCC AGGAATTTAC TGATGGCTCC TTTAGTCAGC CCTTATATCG TGGAGAATTA
    AATGAACACC TGGGGTTGTT GGGCCCATAT ATAAGAGCAG AAGTTGAAGA CAACATTATG
    GTAACTTTCA AAAACCAGGC CTCCCGTCCC TACTCCTTCT ATTCTAGCCT CATTTCTTAT
    AAAGAAGATC AGAGAGGAGA AGAACCTAGA AGAAACTTTG TCAAGCCTAA TGAAACCAAA
    ATTTATTTTT GGAAAGTACA ACATCATATG GCACCCACAG AAGATGAGTT TGACTGCAAG
    GCCTGGGCTT ATTTCTCTGA TGTTGATCTT GAAAGAGATA TGCACTCGGG ATTAATTGGA
    CCCCTTCTGA TTTGCCACGC GAACACACTG AATCCTGCTC ATGGGAGACA AGTGTCAGTA
    CAGGAATTTG CTCTGCTTTT CACTATCTTT GATGAGACCA AGAGCTGGTA CTTCACTGAA
    AACGTGAAAA GGAACTGCAA GACACCCTGC AATTTCCAGA TGGAAGACCC CACTTTGAAA
    GAGAATTATC GCTTCCATGC AATCAATGGT TATGTAATGG ATACCCTACC AGGCTTAGTA
    ATGGCTCAAG ATCAAAGGAT TCGATGGTAT CTTCTCAGCA TGGGCAACAA TGAGAACATC
    CAATCTATTC ATTTCAGTGG ACATGTTTTC ACTGTACGGA AAAAAGAGGA GTATAAAATG
    GCAGTGTACA ACCTCTACCC AGGTGTTTTT GAGACTCTGG AAATGATACC ATCCAGAGCT
    GGAATATGGC GAGTAGAATG CCTTATTGGC GAGCACTTAC AGGCTGGGAT GAGCACTCTT
    TTTCTGGTGT ACAGCAAGCA GTGTCAGATT CCTCTTGGAA TGGCTTCTGG AAGCATCCGT
    GATTTCCAGA TTACAGCTTC AGGACATTAT GGACAGTGGG CCCCAAACCT GGCAAGACTT
    CATTATTCCG GATCAATCAA TGCCTGGAGT ACCAAGGAGC CCTTTTCTTG GATCAAGGTA
    GATCTGTTGG CACCAATGAT TGTTCATGGC ATCAAGACTC AGGGTGCTCG TCAGAAATTT
    TCCAGCCTTT ATATCTCTCA ATTTATCATC ATGTATAGCC TGGATGGGAA GAAGTGGCTG
    AGTTATCAAG GAAATTCCAC TGGAACCTTA ATGGTTTTCT TTGGCAATGT GGACTCATCT
    GGGATTAAGC ATAATAGTTT TAATCCTCCA ATTATTGCTC GATATATCCG TTTGCACCCC
    ACTCATTCTA GCATCCGTAG TACTCTTCGC ATGGAGTTGA TGGGCTGTGA TTTAAACAGT
    TGCAGCATAC CATTGGGAAT GGAAAGTAAA GTAATATCAG ATACACAAAT CACTGCCTCA
    TCCTACTTCA CCAACATGTT TGCTACTTGG TCTCCTTCAC AAGCTCGACT TCACCTCCAG
    GGAAGGACTA ATGCCTGGCG ACCTCAGGTG AATGATCCAA AACAATGGTT GCAAGTGGAC
    TTACAAAAGA CAATGAAAGT CACTGGAATA ATAACCCAGG GAGTGAAATC TCTCTTTACC
    AGCATGTTTG TGAAAGAGTT CCTTATTTCC AGCAGTCAAG ATGGCCATCA CTGGACTCAA
    ATTTTATACA ATGGCAAGGT TTTTCAGGGG AATCAGGACT CATCCACACC TATGATGAAT
    TCTCTAGACC CACCATTACT CACTCGCTAT CTTCGAATTC ACCCCCAGAT CTGGGAGCAC
    CAAATTGCTC TGAGGCTTGA GATTCTAGGA TGTGAGGCCC AGCAGCAATA CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006527852.1
    CDS2603..9556
    Translation

    Target ORF information:

    RefSeq Version XM_006527789.4
    Organism Mus musculus(house mouse)
    Definition Mus musculus coagulation factor VIII (F8), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006527789.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    ATGCAAATAG CACTCTTCGC TTGCTTCTTT CTGAGCCTTT TCAATTTCTG CTCTAGTGCC 
    ATCAGAAGAT ACTACCTTGG TGCAGTGGAA TTGTCCTGGA ACTATATTCA GAGTGATCTG
    CTCAGTGTGC TGCATACAGA CTCAAGATTT CTTCCTAGAA TGTCAACATC TTTTCCATTC
    AACACCTCCA TCATGTATAA AAAGACTGTG TTTGTAGAGT ACAAGGACCA GCTTTTCAAC
    ATTGCCAAGC CCAGGCCACC CTGGATGGGT TTGCTAGGTC CTACCATTTG GACTGAGGTT
    CATGACACAG TGGTCATTAC ACTTAAAAAC ATGGCTTCTC ATCCTGTCAG TCTTCATGCT
    GTTGGTGTGT CCTACTGGAA AGCTTCTGAG GGAGATGAAT ATGAAGATCA GACAAGCCAA
    ATGGAGAAGG AAGATGATAA AGTTTTCCCT GGTGAAAGTC ATACTTATGT TTGGCAAGTC
    CTGAAAGAGA ATGGTCCAAT GGCCTCTGAC CCTCCATGTC TCACTTACTC ATATATGTCT
    CATGTGGATC TGGTGAAAGA TTTGAATTCA GGCCTCATTG GAGCTCTGCT AGTATGTAAA
    GAAGGCAGTC TCTCCAAAGA AAGAACACAG ATGTTGTACC AATTTGTACT GCTTTTTGCT
    GTATTTGATG AAGGGAAGAG CTGGCACTCA GAAACAAACG ACTCTTATAC ACAGTCTATG
    GATTCTGCAT CTGCTAGAGA CTGGCCTAAA ATGCACACAG TCAATGGCTA TGTAAACAGG
    TCTCTTCCAG GTCTGATTGG ATGCCATAGG AAATCAGTCT ACTGGCACGT GATTGGAATG
    GGCACCACTC CTGAAATACA CTCAATATTC CTCGAAGGTC ACACATTTTT TGTGAGGAAC
    CACCGTCAAG CTTCATTGGA GATATCACCA ATAACTTTCC TTACTGCTCA AACACTCTTG
    ATAGATCTTG GGCAGTTCCT ACTATTTTGT CATATCTCTT CCCATAAACA TGATGGCATG
    GAAGCTTATG TCAAAGTAGA TAGCTGCCCT GAGGAATCCC AATGGCAAAA GAAAAATAAT
    AATGAGGAAA TGGAAGATTA TGATGATGAT CTTTATTCAG AAATGGATAT GTTCACATTG
    GATTATGACA GCTCTCCTTT TATCCAAATT CGCTCGGTTG CTAAAAAGTA CCCTAAAACT
    TGGATACATT ATATTTCTGC TGAGGAGGAA GACTGGGACT ATGCACCTTC AGTTCCTACC
    TCGGATAATG GAAGTTATAA AAGCCAGTAT CTGAGCAATG GTCCTCATCG GATTGGTAGG
    AAATATAAAA AAGTCAGATT TATAGCATAC ACAGATGAAA CCTTTAAGAC TCGTGAAACT
    ATTCAGCATG AATCAGGACT CTTGGGACCT TTACTTTATG GAGAAGTTGG AGACACACTG
    TTGATTATTT TTAAGAATCA AGCAAGCCGA CCATATAACA TTTACCCTCA TGGAATCACT
    GATGTCAGTC CTCTACATGC AAGGAGATTG CCAAGAGGTA TAAAGCACGT GAAGGATTTG
    CCAATTCATC CAGGAGAGAT ATTCAAGTAC AAGTGGACAG TTACAGTAGA AGATGGACCA
    ACTAAATCAG ATCCACGGTG CCTGACCCGC TATTATTCAA GTTTCATTAA CCCTGAGAGA
    GATCTAGCTT CAGGACTGAT TGGCCCTCTT CTCATCTGCT ACAAAGAATC TGTAGATCAA
    AGGGGAAACC AGATGATGTC AGACAAAAGA AATGTCATCC TGTTTTCTAT ATTTGATGAG
    AACCAAAGCT GGTACATCAC AGAGAACATG CAACGCTTCC TCCCCAATGC AGCTAAAACA
    CAGCCCCAGG ACCCTGGGTT CCAGGCCTCC AACATCATGC ACAGCATCAA TGGCTATGTT
    TTTGATAGCT TGGAGTTGAC AGTTTGTTTG CATGAGGTGG CATACTGGCA CATTCTCAGT
    GTTGGAGCAC AGACAGACTT CTTATCTATC TTCTTCTCTG GATATACTTT CAAACACAAA
    ATGGTCTATG AAGATACACT TACCCTGTTC CCATTCTCAG GAGAAACTGT CTTTATGTCG
    ATGGAAAACC CAGGTCTATG GGTCTTGGGG TGTCATAATT CAGACTTTCG GAAGAGAGGT
    ATGACAGCAT TGCTGAAAGT TTCTAGTTGT GACAAGAGCA CTAGTGATTA TTATGAAGAA
    ATATATGAAG ATATTCCAAC ACAGTTGGTG AATGAGAACA ATGTCATTGA TCCCAGAAGC
    TTCTTCCAGA ATACAAATCA TCCTAATACT AGGAAAAAGA AATTCAAAGA TTCCACAATT
    CCAAAAAATG ATATGGAGAA GATTGAGCCT CAGTTTGAAG AGATAGCAGA GATGCTTAAA
    GTACAGAGTG TCTCAGTTAG TGACATGTTG ATGCTCTTGG GACAGAGTCA TCCTACTCCA
    CATGGCTTAT TTTTATCAGA TGGCCAAGAA GCCATCTATG AGGCTATTCA TGATGATCAT
    TCACCAAATG CAATAGACAG CAATGAAGGC CCATCTAAAG TGACCCAACT CAGGCCAGAA
    TCCCATCACA GTGAGAAAAT AGTATTTACT CCTCAGCCCG GCCTCCAGTT AAGATCCAAT
    AAAAGTTTGG AGACAACTAT AGAAGTAAAG TGGAAGAAAC TTGGTTTGCA AGTTTCTAGT
    TTGCCAAGTA ATCTAATGAC TACAACAATT CTGTCAGACA ATTTGAAAGC AACTTTTGAA
    AAGACAGATT CTTCAGGATT TCCAGATATG CCAGTTCACT CTAGTAGTAA ATTAAGTACT
    ACTGCATTTG GTAAGAAAGC ATATTCCCTT GTTGGGTCTC ATGTACCTTT AAACGTGAGT
    GAAGAAAATA GTGATTCCAA CATATTGGAT TCAACTTTAA TGTATAGTCA AGAAAGTTTA
    CCAAGAGATA ATATATTATC AATGGAGAAT GATAGATTAC TCAGAGAGAA GAGGTTTCAT
    GGAATTGCTT TATTGACCAA AGATAATACT TTATTCAAAG ACAATGTCTC CTTAATGAAA
    ACAAACAAAA CATATAATCA TTCAACAACT AATGAAAAAC TACACACTGA GAGCCCAACA
    TCAATTGAGA ATAGTACAAC AGACTTGCAA GATGCCATAT TAAAGGTCAA TAGTGAGATT
    CAAGAAGTAA CAGCTTTGAT TCATGATGGA ACACTTTTAG GCAAAAATTC TACATATTTG
    AGACTAAACC ATATGCTAAA TAGAACTACC TCAACAAAAA ATAAAGACAT ATTTCATAGA
    AAAGATGAAG ATCCTATTCC ACAAGATGAA GAGAATACAA TCATGCCATT TTCCAAGATG
    TTGTTCTTGT CAGAATCTTC AAATTGGTTT AAAAAGACCA ATGGAAATAA TTCCTTGAAC
    TCTGAGCAAG AACATAGTCC AAAGCAATTA GTATATTTAA TGTTTAAAAA ATATGTAAAA
    AATCAAAGTT TCTTGTCAGA GAAAAATAAA GTCACAGTAG AACAGGATGG ATTTACAAAG
    AACATAGGAC TTAAAGACAT GGCTTTTCCA CATAATATGA GCATATTTCT TACCACTTTG
    TCTAACGTAC ATGAAAATGG TAGGCACAAT CAAGAAAAAA ATATTCAGGA AGAGATAGAG
    AAGGAAGCAC TAATTGAAGA GAAAGTAGTT TTGCCCCAGG TGCACGAAGC AACTGGCTCT
    AAGAATTTCT TGAAAGACAT ATTGATACTA GGCACTAGGC AAAATATAAG TTTATATGAA
    GTACATGTAC CAGTACTTCA AAACATCACA TCAATAAACA ATTCAACAAA TACAGTACAG
    ATTCACATGG AGCATTTCTT TAAAAGAAGG AAGGACAAGG AAACAAATTC AGAAGGCTTG
    GTAAATAAAA CCAGAGAAAT GGTAAAAAAC TATCCAAGCC AGAAGAATAT TACTACTCAA
    CGTAGTAAAC GGGCTTTGGG ACAATTCAGA CTGTCAACTC AATGGCTTAA AACCATAAAC
    TGTTCAACAC AGTGTATCAT TAAACAGATA GACCACAGCA AGGAAATGAA AAAGTTCATT
    ACTAAATCTT CCTTATCAGA TTCTTCTGTG ATTAAAAGCA CCACTCAGAC AAATAGTTCT
    GACTCACACA TTGTAAAAAC ATCAGCATTT CCACCAATAG ATCTCAAAAG GAGTCCATTC
    CAAAACAAAT TTTCTCATGT TCAAGCATCA TCCTACATTT ATGACTTTAA GACAAAAAGT
    TCAAGAATTC AAGAAAGCAA TAATTTCTTA AAAGAAACCA AAATAAATAA CCCTTCTTTA
    GCCATTCTAC CATGGAATAT GTTCATAGAT CAAGGAAAAT TTACCTCCCC AGGGAAAAGT
    AACACAAACT CAGTCACATA TAAGAAACGT GAGAACATTA TTTTCTTGAA ACCAACTTTG
    CCTGAAGAAT CTGGCAAAAT TGAATTGCTT CCTCAAGTTT CCATTCAAGA GGAAGAAATT
    TTACCTACAG AAACTAGCCA TGGATCTCCT GGACACTTGA ATCTCATGAA AGAGGTCTTT
    CTTCAGAAAA TACAGGGGCC TACTAAATGG AATAAAGCAA AGAGGCATGG AGAAAGTATA
    AAAGGTAAAA CAGAGAGCTC TAAAAATACT CGCTCAAAAC TGCTAAATCA TCATGCTTGG
    GATTATCATT ATGCTGCACA GATACCAAAA GATATGTGGA AATCCAAAGA GAAGTCACCA
    GAAATTATAT CCATTAAGCA AGAGGACACC ATTTTGTCTC TGAGGCCTCA TGGAAACAGT
    CATTCAATAG GGGCAAATGA GAAACAAAAT TGGCCTCAAA GAGAAACCAC TTGGGTAAAG
    CAAGGCCAAA CTCAAAGGAC ATGCTCTCAA ATCCCACCAG TGTTGAAACG ACATCAAAGG
    GAACTTAGTG CTTTTCAATC AGAACAAGAA GCAACTGACT ATGATGATGC CATCACCATT
    GAAACAATCG AGGATTTTGA CATTTACAGT GAGGACATAA AGCAAGGTCC CCGCAGCTTT
    CAACAGAAAA CAAGGCACTA TTTTATTGCA GCTGTGGAAC GACTCTGGGA CTATGGGATG
    AGTACATCTC ATGTTCTACG AAATAGGTAT CAAAGTGACA ATGTACCTCA GTTCAAGAAA
    GTAGTTTTCC AGGAATTTAC TGATGGCTCC TTTAGTCAGC CCTTATATCG TGGAGAATTA
    AATGAACACC TGGGGTTGTT GGGCCCATAT ATAAGAGCAG AAGTTGAAGA CAACATTATG
    GTAACTTTCA AAAACCAGGC CTCCCGTCCC TACTCCTTCT ATTCTAGCCT CATTTCTTAT
    AAAGAAGATC AGAGAGGAGA AGAACCTAGA AGAAACTTTG TCAAGCCTAA TGAAACCAAA
    ATTTATTTTT GGAAAGTACA ACATCATATG GCACCCACAG AAGATGAGTT TGACTGCAAG
    GCCTGGGCTT ATTTCTCTGA TGTTGATCTT GAAAGAGATA TGCACTCGGG ATTAATTGGA
    CCCCTTCTGA TTTGCCACGC GAACACACTG AATCCTGCTC ATGGGAGACA AGTGTCAGTA
    CAGGAATTTG CTCTGCTTTT CACTATCTTT GATGAGACCA AGAGCTGGTA CTTCACTGAA
    AACGTGAAAA GGAACTGCAA GACACCCTGC AATTTCCAGA TGGAAGACCC CACTTTGAAA
    GAGAATTATC GCTTCCATGC AATCAATGGT TATGTAATGG ATACCCTACC AGGCTTAGTA
    ATGGCTCAAG ATCAAAGGAT TCGATGGTAT CTTCTCAGCA TGGGCAACAA TGAGAACATC
    CAATCTATTC ATTTCAGTGG ACATGTTTTC ACTGTACGGA AAAAAGAGGA GTATAAAATG
    GCAGTGTACA ACCTCTACCC AGGTGTTTTT GAGACTCTGG AAATGATACC ATCCAGAGCT
    GGAATATGGC GAGTAGAATG CCTTATTGGC GAGCACTTAC AGGCTGGGAT GAGCACTCTT
    TTTCTGGTGT ACAGCAAGCA GTGTCAGATT CCTCTTGGAA TGGCTTCTGG AAGCATCCGT
    GATTTCCAGA TTACAGCTTC AGGACATTAT GGACAGTGGG CCCCAAACCT GGCAAGACTT
    CATTATTCCG GATCAATCAA TGCCTGGAGT ACCAAGGAGC CCTTTTCTTG GATCAAGGTA
    GATCTGTTGG CACCAATGAT TGTTCATGGC ATCAAGACTC AGGGTGCTCG TCAGAAATTT
    TCCAGCCTTT ATATCTCTCA ATTTATCATC ATGTATAGCC TGGATGGGAA GAAGTGGCTG
    AGTTATCAAG GAAATTCCAC TGGAACCTTA ATGGTTTTCT TTGGCAATGT GGACTCATCT
    GGGATTAAGC ATAATAGTTT TAATCCTCCA ATTATTGCTC GATATATCCG TTTGCACCCC
    ACTCATTCTA GCATCCGTAG TACTCTTCGC ATGGAGTTGA TGGGCTGTGA TTTAAACAGT
    TGCAGCATAC CATTGGGAAT GGAAAGTAAA GTAATATCAG ATACACAAAT CACTGCCTCA
    TCCTACTTCA CCAACATGTT TGCTACTTGG TCTCCTTCAC AAGCTCGACT TCACCTCCAG
    GGAAGGACTA ATGCCTGGCG ACCTCAGGTG AATGATCCAA AACAATGGTT GCAAGTGGAC
    TTACAAAAGA CAATGAAAGT CACTGGAATA ATAACCCAGG GAGTGAAATC TCTCTTTACC
    AGCATGTTTG TGAAAGAGTT CCTTATTTCC AGCAGTCAAG ATGGCCATCA CTGGACTCAA
    ATTTTATACA ATGGCAAGGT TTTTCAGGGG AATCAGGACT CATCCACACC TATGATGAAT
    TCTCTAGACC CACCATTACT CACTCGCTAT CTTCGAATTC ACCCCCAGAT CTGGGAGCAC
    CAAATTGCTC TGAGGCTTGA GATTCTAGGA TGTGAGGCCC AGCAGCAATA CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu56129
    Clone ID Related Accession (Same CDS sequence) XM_006527790.4 , XM_006527790.3
    Accession Version XM_006527790.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6939bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product coagulation factor VIII isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000086.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006527790.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    ATGCAACCAG CAGTACTTCA CTCTAAGCGA CAAAGTGCCA TCAGAAGATA CTACCTTGGT 
    GCAGTGGAAT TGTCCTGGAA CTATATTCAG AGTGATCTGC TCAGTGTGCT GCATACAGAC
    TCAAGATTTC TTCCTAGAAT GTCAACATCT TTTCCATTCA ACACCTCCAT CATGTATAAA
    AAGACTGTGT TTGTAGAGTA CAAGGACCAG CTTTTCAACA TTGCCAAGCC CAGGCCACCC
    TGGATGGGTT TGCTAGGTCC TACCATTTGG ACTGAGGTTC ATGACACAGT GGTCATTACA
    CTTAAAAACA TGGCTTCTCA TCCTGTCAGT CTTCATGCTG TTGGTGTGTC CTACTGGAAA
    GCTTCTGAGG GAGATGAATA TGAAGATCAG ACAAGCCAAA TGGAGAAGGA AGATGATAAA
    GTTTTCCCTG GTGAAAGTCA TACTTATGTT TGGCAAGTCC TGAAAGAGAA TGGTCCAATG
    GCCTCTGACC CTCCATGTCT CACTTACTCA TATATGTCTC ATGTGGATCT GGTGAAAGAT
    TTGAATTCAG GCCTCATTGG AGCTCTGCTA GTATGTAAAG AAGGCAGTCT CTCCAAAGAA
    AGAACACAGA TGTTGTACCA ATTTGTACTG CTTTTTGCTG TATTTGATGA AGGGAAGAGC
    TGGCACTCAG AAACAAACGA CTCTTATACA CAGTCTATGG ATTCTGCATC TGCTAGAGAC
    TGGCCTAAAA TGCACACAGT CAATGGCTAT GTAAACAGGT CTCTTCCAGG TCTGATTGGA
    TGCCATAGGA AATCAGTCTA CTGGCACGTG ATTGGAATGG GCACCACTCC TGAAATACAC
    TCAATATTCC TCGAAGGTCA CACATTTTTT GTGAGGAACC ACCGTCAAGC TTCATTGGAG
    ATATCACCAA TAACTTTCCT TACTGCTCAA ACACTCTTGA TAGATCTTGG GCAGTTCCTA
    CTATTTTGTC ATATCTCTTC CCATAAACAT GATGGCATGG AAGCTTATGT CAAAGTAGAT
    AGCTGCCCTG AGGAATCCCA ATGGCAAAAG AAAAATAATA ATGAGGAAAT GGAAGATTAT
    GATGATGATC TTTATTCAGA AATGGATATG TTCACATTGG ATTATGACAG CTCTCCTTTT
    ATCCAAATTC GCTCGGTTGC TAAAAAGTAC CCTAAAACTT GGATACATTA TATTTCTGCT
    GAGGAGGAAG ACTGGGACTA TGCACCTTCA GTTCCTACCT CGGATAATGG AAGTTATAAA
    AGCCAGTATC TGAGCAATGG TCCTCATCGG ATTGGTAGGA AATATAAAAA AGTCAGATTT
    ATAGCATACA CAGATGAAAC CTTTAAGACT CGTGAAACTA TTCAGCATGA ATCAGGACTC
    TTGGGACCTT TACTTTATGG AGAAGTTGGA GACACACTGT TGATTATTTT TAAGAATCAA
    GCAAGCCGAC CATATAACAT TTACCCTCAT GGAATCACTG ATGTCAGTCC TCTACATGCA
    AGGAGATTGC CAAGAGGTAT AAAGCACGTG AAGGATTTGC CAATTCATCC AGGAGAGATA
    TTCAAGTACA AGTGGACAGT TACAGTAGAA GATGGACCAA CTAAATCAGA TCCACGGTGC
    CTGACCCGCT ATTATTCAAG TTTCATTAAC CCTGAGAGAG ATCTAGCTTC AGGACTGATT
    GGCCCTCTTC TCATCTGCTA CAAAGAATCT GTAGATCAAA GGGGAAACCA GATGATGTCA
    GACAAAAGAA ATGTCATCCT GTTTTCTATA TTTGATGAGA ACCAAAGCTG GTACATCACA
    GAGAACATGC AACGCTTCCT CCCCAATGCA GCTAAAACAC AGCCCCAGGA CCCTGGGTTC
    CAGGCCTCCA ACATCATGCA CAGCATCAAT GGCTATGTTT TTGATAGCTT GGAGTTGACA
    GTTTGTTTGC ATGAGGTGGC ATACTGGCAC ATTCTCAGTG TTGGAGCACA GACAGACTTC
    TTATCTATCT TCTTCTCTGG ATATACTTTC AAACACAAAA TGGTCTATGA AGATACACTT
    ACCCTGTTCC CATTCTCAGG AGAAACTGTC TTTATGTCGA TGGAAAACCC AGGTCTATGG
    GTCTTGGGGT GTCATAATTC AGACTTTCGG AAGAGAGGTA TGACAGCATT GCTGAAAGTT
    TCTAGTTGTG ACAAGAGCAC TAGTGATTAT TATGAAGAAA TATATGAAGA TATTCCAACA
    CAGTTGGTGA ATGAGAACAA TGTCATTGAT CCCAGAAGCT TCTTCCAGAA TACAAATCAT
    CCTAATACTA GGAAAAAGAA ATTCAAAGAT TCCACAATTC CAAAAAATGA TATGGAGAAG
    ATTGAGCCTC AGTTTGAAGA GATAGCAGAG ATGCTTAAAG TACAGAGTGT CTCAGTTAGT
    GACATGTTGA TGCTCTTGGG ACAGAGTCAT CCTACTCCAC ATGGCTTATT TTTATCAGAT
    GGCCAAGAAG CCATCTATGA GGCTATTCAT GATGATCATT CACCAAATGC AATAGACAGC
    AATGAAGGCC CATCTAAAGT GACCCAACTC AGGCCAGAAT CCCATCACAG TGAGAAAATA
    GTATTTACTC CTCAGCCCGG CCTCCAGTTA AGATCCAATA AAAGTTTGGA GACAACTATA
    GAAGTAAAGT GGAAGAAACT TGGTTTGCAA GTTTCTAGTT TGCCAAGTAA TCTAATGACT
    ACAACAATTC TGTCAGACAA TTTGAAAGCA ACTTTTGAAA AGACAGATTC TTCAGGATTT
    CCAGATATGC CAGTTCACTC TAGTAGTAAA TTAAGTACTA CTGCATTTGG TAAGAAAGCA
    TATTCCCTTG TTGGGTCTCA TGTACCTTTA AACGTGAGTG AAGAAAATAG TGATTCCAAC
    ATATTGGATT CAACTTTAAT GTATAGTCAA GAAAGTTTAC CAAGAGATAA TATATTATCA
    ATGGAGAATG ATAGATTACT CAGAGAGAAG AGGTTTCATG GAATTGCTTT ATTGACCAAA
    GATAATACTT TATTCAAAGA CAATGTCTCC TTAATGAAAA CAAACAAAAC ATATAATCAT
    TCAACAACTA ATGAAAAACT ACACACTGAG AGCCCAACAT CAATTGAGAA TAGTACAACA
    GACTTGCAAG ATGCCATATT AAAGGTCAAT AGTGAGATTC AAGAAGTAAC AGCTTTGATT
    CATGATGGAA CACTTTTAGG CAAAAATTCT ACATATTTGA GACTAAACCA TATGCTAAAT
    AGAACTACCT CAACAAAAAA TAAAGACATA TTTCATAGAA AAGATGAAGA TCCTATTCCA
    CAAGATGAAG AGAATACAAT CATGCCATTT TCCAAGATGT TGTTCTTGTC AGAATCTTCA
    AATTGGTTTA AAAAGACCAA TGGAAATAAT TCCTTGAACT CTGAGCAAGA ACATAGTCCA
    AAGCAATTAG TATATTTAAT GTTTAAAAAA TATGTAAAAA ATCAAAGTTT CTTGTCAGAG
    AAAAATAAAG TCACAGTAGA ACAGGATGGA TTTACAAAGA ACATAGGACT TAAAGACATG
    GCTTTTCCAC ATAATATGAG CATATTTCTT ACCACTTTGT CTAACGTACA TGAAAATGGT
    AGGCACAATC AAGAAAAAAA TATTCAGGAA GAGATAGAGA AGGAAGCACT AATTGAAGAG
    AAAGTAGTTT TGCCCCAGGT GCACGAAGCA ACTGGCTCTA AGAATTTCTT GAAAGACATA
    TTGATACTAG GCACTAGGCA AAATATAAGT TTATATGAAG TACATGTACC AGTACTTCAA
    AACATCACAT CAATAAACAA TTCAACAAAT ACAGTACAGA TTCACATGGA GCATTTCTTT
    AAAAGAAGGA AGGACAAGGA AACAAATTCA GAAGGCTTGG TAAATAAAAC CAGAGAAATG
    GTAAAAAACT ATCCAAGCCA GAAGAATATT ACTACTCAAC GTAGTAAACG GGCTTTGGGA
    CAATTCAGAC TGTCAACTCA ATGGCTTAAA ACCATAAACT GTTCAACACA GTGTATCATT
    AAACAGATAG ACCACAGCAA GGAAATGAAA AAGTTCATTA CTAAATCTTC CTTATCAGAT
    TCTTCTGTGA TTAAAAGCAC CACTCAGACA AATAGTTCTG ACTCACACAT TGTAAAAACA
    TCAGCATTTC CACCAATAGA TCTCAAAAGG AGTCCATTCC AAAACAAATT TTCTCATGTT
    CAAGCATCAT CCTACATTTA TGACTTTAAG ACAAAAAGTT CAAGAATTCA AGAAAGCAAT
    AATTTCTTAA AAGAAACCAA AATAAATAAC CCTTCTTTAG CCATTCTACC ATGGAATATG
    TTCATAGATC AAGGAAAATT TACCTCCCCA GGGAAAAGTA ACACAAACTC AGTCACATAT
    AAGAAACGTG AGAACATTAT TTTCTTGAAA CCAACTTTGC CTGAAGAATC TGGCAAAATT
    GAATTGCTTC CTCAAGTTTC CATTCAAGAG GAAGAAATTT TACCTACAGA AACTAGCCAT
    GGATCTCCTG GACACTTGAA TCTCATGAAA GAGGTCTTTC TTCAGAAAAT ACAGGGGCCT
    ACTAAATGGA ATAAAGCAAA GAGGCATGGA GAAAGTATAA AAGGTAAAAC AGAGAGCTCT
    AAAAATACTC GCTCAAAACT GCTAAATCAT CATGCTTGGG ATTATCATTA TGCTGCACAG
    ATACCAAAAG ATATGTGGAA ATCCAAAGAG AAGTCACCAG AAATTATATC CATTAAGCAA
    GAGGACACCA TTTTGTCTCT GAGGCCTCAT GGAAACAGTC ATTCAATAGG GGCAAATGAG
    AAACAAAATT GGCCTCAAAG AGAAACCACT TGGGTAAAGC AAGGCCAAAC TCAAAGGACA
    TGCTCTCAAA TCCCACCAGT GTTGAAACGA CATCAAAGGG AACTTAGTGC TTTTCAATCA
    GAACAAGAAG CAACTGACTA TGATGATGCC ATCACCATTG AAACAATCGA GGATTTTGAC
    ATTTACAGTG AGGACATAAA GCAAGGTCCC CGCAGCTTTC AACAGAAAAC AAGGCACTAT
    TTTATTGCAG CTGTGGAACG ACTCTGGGAC TATGGGATGA GTACATCTCA TGTTCTACGA
    AATAGGTATC AAAGTGACAA TGTACCTCAG TTCAAGAAAG TAGTTTTCCA GGAATTTACT
    GATGGCTCCT TTAGTCAGCC CTTATATCGT GGAGAATTAA ATGAACACCT GGGGTTGTTG
    GGCCCATATA TAAGAGCAGA AGTTGAAGAC AACATTATGG TAACTTTCAA AAACCAGGCC
    TCCCGTCCCT ACTCCTTCTA TTCTAGCCTC ATTTCTTATA AAGAAGATCA GAGAGGAGAA
    GAACCTAGAA GAAACTTTGT CAAGCCTAAT GAAACCAAAA TTTATTTTTG GAAAGTACAA
    CATCATATGG CACCCACAGA AGATGAGTTT GACTGCAAGG CCTGGGCTTA TTTCTCTGAT
    GTTGATCTTG AAAGAGATAT GCACTCGGGA TTAATTGGAC CCCTTCTGAT TTGCCACGCG
    AACACACTGA ATCCTGCTCA TGGGAGACAA GTGTCAGTAC AGGAATTTGC TCTGCTTTTC
    ACTATCTTTG ATGAGACCAA GAGCTGGTAC TTCACTGAAA ACGTGAAAAG GAACTGCAAG
    ACACCCTGCA ATTTCCAGAT GGAAGACCCC ACTTTGAAAG AGAATTATCG CTTCCATGCA
    ATCAATGGTT ATGTAATGGA TACCCTACCA GGCTTAGTAA TGGCTCAAGA TCAAAGGATT
    CGATGGTATC TTCTCAGCAT GGGCAACAAT GAGAACATCC AATCTATTCA TTTCAGTGGA
    CATGTTTTCA CTGTACGGAA AAAAGAGGAG TATAAAATGG CAGTGTACAA CCTCTACCCA
    GGTGTTTTTG AGACTCTGGA AATGATACCA TCCAGAGCTG GAATATGGCG AGTAGAATGC
    CTTATTGGCG AGCACTTACA GGCTGGGATG AGCACTCTTT TTCTGGTGTA CAGCAAGCAG
    TGTCAGATTC CTCTTGGAAT GGCTTCTGGA AGCATCCGTG ATTTCCAGAT TACAGCTTCA
    GGACATTATG GACAGTGGGC CCCAAACCTG GCAAGACTTC ATTATTCCGG ATCAATCAAT
    GCCTGGAGTA CCAAGGAGCC CTTTTCTTGG ATCAAGGTAG ATCTGTTGGC ACCAATGATT
    GTTCATGGCA TCAAGACTCA GGGTGCTCGT CAGAAATTTT CCAGCCTTTA TATCTCTCAA
    TTTATCATCA TGTATAGCCT GGATGGGAAG AAGTGGCTGA GTTATCAAGG AAATTCCACT
    GGAACCTTAA TGGTTTTCTT TGGCAATGTG GACTCATCTG GGATTAAGCA TAATAGTTTT
    AATCCTCCAA TTATTGCTCG ATATATCCGT TTGCACCCCA CTCATTCTAG CATCCGTAGT
    ACTCTTCGCA TGGAGTTGAT GGGCTGTGAT TTAAACAGTT GCAGCATACC ATTGGGAATG
    GAAAGTAAAG TAATATCAGA TACACAAATC ACTGCCTCAT CCTACTTCAC CAACATGTTT
    GCTACTTGGT CTCCTTCACA AGCTCGACTT CACCTCCAGG GAAGGACTAA TGCCTGGCGA
    CCTCAGGTGA ATGATCCAAA ACAATGGTTG CAAGTGGACT TACAAAAGAC AATGAAAGTC
    ACTGGAATAA TAACCCAGGG AGTGAAATCT CTCTTTACCA GCATGTTTGT GAAAGAGTTC
    CTTATTTCCA GCAGTCAAGA TGGCCATCAC TGGACTCAAA TTTTATACAA TGGCAAGGTA
    AAGGTTTTTC AGGGGAATCA GGACTCATCC ACACCTATGA TGAATTCTCT AGACCCACCA
    TTACTCACTC GCTATCTTCG AATTCACCCC CAGATCTGGG AGCACCAAAT TGCTCTGAGG
    CTTGAGATTC TAGGATGTGA GGCCCAGCAG CAATACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006527853.1
    CDS210..7148
    Translation

    Target ORF information:

    RefSeq Version XM_006527790.4
    Organism Mus musculus(house mouse)
    Definition Mus musculus coagulation factor VIII (F8), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006527790.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    ATGCAACCAG CAGTACTTCA CTCTAAGCGA CAAAGTGCCA TCAGAAGATA CTACCTTGGT 
    GCAGTGGAAT TGTCCTGGAA CTATATTCAG AGTGATCTGC TCAGTGTGCT GCATACAGAC
    TCAAGATTTC TTCCTAGAAT GTCAACATCT TTTCCATTCA ACACCTCCAT CATGTATAAA
    AAGACTGTGT TTGTAGAGTA CAAGGACCAG CTTTTCAACA TTGCCAAGCC CAGGCCACCC
    TGGATGGGTT TGCTAGGTCC TACCATTTGG ACTGAGGTTC ATGACACAGT GGTCATTACA
    CTTAAAAACA TGGCTTCTCA TCCTGTCAGT CTTCATGCTG TTGGTGTGTC CTACTGGAAA
    GCTTCTGAGG GAGATGAATA TGAAGATCAG ACAAGCCAAA TGGAGAAGGA AGATGATAAA
    GTTTTCCCTG GTGAAAGTCA TACTTATGTT TGGCAAGTCC TGAAAGAGAA TGGTCCAATG
    GCCTCTGACC CTCCATGTCT CACTTACTCA TATATGTCTC ATGTGGATCT GGTGAAAGAT
    TTGAATTCAG GCCTCATTGG AGCTCTGCTA GTATGTAAAG AAGGCAGTCT CTCCAAAGAA
    AGAACACAGA TGTTGTACCA ATTTGTACTG CTTTTTGCTG TATTTGATGA AGGGAAGAGC
    TGGCACTCAG AAACAAACGA CTCTTATACA CAGTCTATGG ATTCTGCATC TGCTAGAGAC
    TGGCCTAAAA TGCACACAGT CAATGGCTAT GTAAACAGGT CTCTTCCAGG TCTGATTGGA
    TGCCATAGGA AATCAGTCTA CTGGCACGTG ATTGGAATGG GCACCACTCC TGAAATACAC
    TCAATATTCC TCGAAGGTCA CACATTTTTT GTGAGGAACC ACCGTCAAGC TTCATTGGAG
    ATATCACCAA TAACTTTCCT TACTGCTCAA ACACTCTTGA TAGATCTTGG GCAGTTCCTA
    CTATTTTGTC ATATCTCTTC CCATAAACAT GATGGCATGG AAGCTTATGT CAAAGTAGAT
    AGCTGCCCTG AGGAATCCCA ATGGCAAAAG AAAAATAATA ATGAGGAAAT GGAAGATTAT
    GATGATGATC TTTATTCAGA AATGGATATG TTCACATTGG ATTATGACAG CTCTCCTTTT
    ATCCAAATTC GCTCGGTTGC TAAAAAGTAC CCTAAAACTT GGATACATTA TATTTCTGCT
    GAGGAGGAAG ACTGGGACTA TGCACCTTCA GTTCCTACCT CGGATAATGG AAGTTATAAA
    AGCCAGTATC TGAGCAATGG TCCTCATCGG ATTGGTAGGA AATATAAAAA AGTCAGATTT
    ATAGCATACA CAGATGAAAC CTTTAAGACT CGTGAAACTA TTCAGCATGA ATCAGGACTC
    TTGGGACCTT TACTTTATGG AGAAGTTGGA GACACACTGT TGATTATTTT TAAGAATCAA
    GCAAGCCGAC CATATAACAT TTACCCTCAT GGAATCACTG ATGTCAGTCC TCTACATGCA
    AGGAGATTGC CAAGAGGTAT AAAGCACGTG AAGGATTTGC CAATTCATCC AGGAGAGATA
    TTCAAGTACA AGTGGACAGT TACAGTAGAA GATGGACCAA CTAAATCAGA TCCACGGTGC
    CTGACCCGCT ATTATTCAAG TTTCATTAAC CCTGAGAGAG ATCTAGCTTC AGGACTGATT
    GGCCCTCTTC TCATCTGCTA CAAAGAATCT GTAGATCAAA GGGGAAACCA GATGATGTCA
    GACAAAAGAA ATGTCATCCT GTTTTCTATA TTTGATGAGA ACCAAAGCTG GTACATCACA
    GAGAACATGC AACGCTTCCT CCCCAATGCA GCTAAAACAC AGCCCCAGGA CCCTGGGTTC
    CAGGCCTCCA ACATCATGCA CAGCATCAAT GGCTATGTTT TTGATAGCTT GGAGTTGACA
    GTTTGTTTGC ATGAGGTGGC ATACTGGCAC ATTCTCAGTG TTGGAGCACA GACAGACTTC
    TTATCTATCT TCTTCTCTGG ATATACTTTC AAACACAAAA TGGTCTATGA AGATACACTT
    ACCCTGTTCC CATTCTCAGG AGAAACTGTC TTTATGTCGA TGGAAAACCC AGGTCTATGG
    GTCTTGGGGT GTCATAATTC AGACTTTCGG AAGAGAGGTA TGACAGCATT GCTGAAAGTT
    TCTAGTTGTG ACAAGAGCAC TAGTGATTAT TATGAAGAAA TATATGAAGA TATTCCAACA
    CAGTTGGTGA ATGAGAACAA TGTCATTGAT CCCAGAAGCT TCTTCCAGAA TACAAATCAT
    CCTAATACTA GGAAAAAGAA ATTCAAAGAT TCCACAATTC CAAAAAATGA TATGGAGAAG
    ATTGAGCCTC AGTTTGAAGA GATAGCAGAG ATGCTTAAAG TACAGAGTGT CTCAGTTAGT
    GACATGTTGA TGCTCTTGGG ACAGAGTCAT CCTACTCCAC ATGGCTTATT TTTATCAGAT
    GGCCAAGAAG CCATCTATGA GGCTATTCAT GATGATCATT CACCAAATGC AATAGACAGC
    AATGAAGGCC CATCTAAAGT GACCCAACTC AGGCCAGAAT CCCATCACAG TGAGAAAATA
    GTATTTACTC CTCAGCCCGG CCTCCAGTTA AGATCCAATA AAAGTTTGGA GACAACTATA
    GAAGTAAAGT GGAAGAAACT TGGTTTGCAA GTTTCTAGTT TGCCAAGTAA TCTAATGACT
    ACAACAATTC TGTCAGACAA TTTGAAAGCA ACTTTTGAAA AGACAGATTC TTCAGGATTT
    CCAGATATGC CAGTTCACTC TAGTAGTAAA TTAAGTACTA CTGCATTTGG TAAGAAAGCA
    TATTCCCTTG TTGGGTCTCA TGTACCTTTA AACGTGAGTG AAGAAAATAG TGATTCCAAC
    ATATTGGATT CAACTTTAAT GTATAGTCAA GAAAGTTTAC CAAGAGATAA TATATTATCA
    ATGGAGAATG ATAGATTACT CAGAGAGAAG AGGTTTCATG GAATTGCTTT ATTGACCAAA
    GATAATACTT TATTCAAAGA CAATGTCTCC TTAATGAAAA CAAACAAAAC ATATAATCAT
    TCAACAACTA ATGAAAAACT ACACACTGAG AGCCCAACAT CAATTGAGAA TAGTACAACA
    GACTTGCAAG ATGCCATATT AAAGGTCAAT AGTGAGATTC AAGAAGTAAC AGCTTTGATT
    CATGATGGAA CACTTTTAGG CAAAAATTCT ACATATTTGA GACTAAACCA TATGCTAAAT
    AGAACTACCT CAACAAAAAA TAAAGACATA TTTCATAGAA AAGATGAAGA TCCTATTCCA
    CAAGATGAAG AGAATACAAT CATGCCATTT TCCAAGATGT TGTTCTTGTC AGAATCTTCA
    AATTGGTTTA AAAAGACCAA TGGAAATAAT TCCTTGAACT CTGAGCAAGA ACATAGTCCA
    AAGCAATTAG TATATTTAAT GTTTAAAAAA TATGTAAAAA ATCAAAGTTT CTTGTCAGAG
    AAAAATAAAG TCACAGTAGA ACAGGATGGA TTTACAAAGA ACATAGGACT TAAAGACATG
    GCTTTTCCAC ATAATATGAG CATATTTCTT ACCACTTTGT CTAACGTACA TGAAAATGGT
    AGGCACAATC AAGAAAAAAA TATTCAGGAA GAGATAGAGA AGGAAGCACT AATTGAAGAG
    AAAGTAGTTT TGCCCCAGGT GCACGAAGCA ACTGGCTCTA AGAATTTCTT GAAAGACATA
    TTGATACTAG GCACTAGGCA AAATATAAGT TTATATGAAG TACATGTACC AGTACTTCAA
    AACATCACAT CAATAAACAA TTCAACAAAT ACAGTACAGA TTCACATGGA GCATTTCTTT
    AAAAGAAGGA AGGACAAGGA AACAAATTCA GAAGGCTTGG TAAATAAAAC CAGAGAAATG
    GTAAAAAACT ATCCAAGCCA GAAGAATATT ACTACTCAAC GTAGTAAACG GGCTTTGGGA
    CAATTCAGAC TGTCAACTCA ATGGCTTAAA ACCATAAACT GTTCAACACA GTGTATCATT
    AAACAGATAG ACCACAGCAA GGAAATGAAA AAGTTCATTA CTAAATCTTC CTTATCAGAT
    TCTTCTGTGA TTAAAAGCAC CACTCAGACA AATAGTTCTG ACTCACACAT TGTAAAAACA
    TCAGCATTTC CACCAATAGA TCTCAAAAGG AGTCCATTCC AAAACAAATT TTCTCATGTT
    CAAGCATCAT CCTACATTTA TGACTTTAAG ACAAAAAGTT CAAGAATTCA AGAAAGCAAT
    AATTTCTTAA AAGAAACCAA AATAAATAAC CCTTCTTTAG CCATTCTACC ATGGAATATG
    TTCATAGATC AAGGAAAATT TACCTCCCCA GGGAAAAGTA ACACAAACTC AGTCACATAT
    AAGAAACGTG AGAACATTAT TTTCTTGAAA CCAACTTTGC CTGAAGAATC TGGCAAAATT
    GAATTGCTTC CTCAAGTTTC CATTCAAGAG GAAGAAATTT TACCTACAGA AACTAGCCAT
    GGATCTCCTG GACACTTGAA TCTCATGAAA GAGGTCTTTC TTCAGAAAAT ACAGGGGCCT
    ACTAAATGGA ATAAAGCAAA GAGGCATGGA GAAAGTATAA AAGGTAAAAC AGAGAGCTCT
    AAAAATACTC GCTCAAAACT GCTAAATCAT CATGCTTGGG ATTATCATTA TGCTGCACAG
    ATACCAAAAG ATATGTGGAA ATCCAAAGAG AAGTCACCAG AAATTATATC CATTAAGCAA
    GAGGACACCA TTTTGTCTCT GAGGCCTCAT GGAAACAGTC ATTCAATAGG GGCAAATGAG
    AAACAAAATT GGCCTCAAAG AGAAACCACT TGGGTAAAGC AAGGCCAAAC TCAAAGGACA
    TGCTCTCAAA TCCCACCAGT GTTGAAACGA CATCAAAGGG AACTTAGTGC TTTTCAATCA
    GAACAAGAAG CAACTGACTA TGATGATGCC ATCACCATTG AAACAATCGA GGATTTTGAC
    ATTTACAGTG AGGACATAAA GCAAGGTCCC CGCAGCTTTC AACAGAAAAC AAGGCACTAT
    TTTATTGCAG CTGTGGAACG ACTCTGGGAC TATGGGATGA GTACATCTCA TGTTCTACGA
    AATAGGTATC AAAGTGACAA TGTACCTCAG TTCAAGAAAG TAGTTTTCCA GGAATTTACT
    GATGGCTCCT TTAGTCAGCC CTTATATCGT GGAGAATTAA ATGAACACCT GGGGTTGTTG
    GGCCCATATA TAAGAGCAGA AGTTGAAGAC AACATTATGG TAACTTTCAA AAACCAGGCC
    TCCCGTCCCT ACTCCTTCTA TTCTAGCCTC ATTTCTTATA AAGAAGATCA GAGAGGAGAA
    GAACCTAGAA GAAACTTTGT CAAGCCTAAT GAAACCAAAA TTTATTTTTG GAAAGTACAA
    CATCATATGG CACCCACAGA AGATGAGTTT GACTGCAAGG CCTGGGCTTA TTTCTCTGAT
    GTTGATCTTG AAAGAGATAT GCACTCGGGA TTAATTGGAC CCCTTCTGAT TTGCCACGCG
    AACACACTGA ATCCTGCTCA TGGGAGACAA GTGTCAGTAC AGGAATTTGC TCTGCTTTTC
    ACTATCTTTG ATGAGACCAA GAGCTGGTAC TTCACTGAAA ACGTGAAAAG GAACTGCAAG
    ACACCCTGCA ATTTCCAGAT GGAAGACCCC ACTTTGAAAG AGAATTATCG CTTCCATGCA
    ATCAATGGTT ATGTAATGGA TACCCTACCA GGCTTAGTAA TGGCTCAAGA TCAAAGGATT
    CGATGGTATC TTCTCAGCAT GGGCAACAAT GAGAACATCC AATCTATTCA TTTCAGTGGA
    CATGTTTTCA CTGTACGGAA AAAAGAGGAG TATAAAATGG CAGTGTACAA CCTCTACCCA
    GGTGTTTTTG AGACTCTGGA AATGATACCA TCCAGAGCTG GAATATGGCG AGTAGAATGC
    CTTATTGGCG AGCACTTACA GGCTGGGATG AGCACTCTTT TTCTGGTGTA CAGCAAGCAG
    TGTCAGATTC CTCTTGGAAT GGCTTCTGGA AGCATCCGTG ATTTCCAGAT TACAGCTTCA
    GGACATTATG GACAGTGGGC CCCAAACCTG GCAAGACTTC ATTATTCCGG ATCAATCAAT
    GCCTGGAGTA CCAAGGAGCC CTTTTCTTGG ATCAAGGTAG ATCTGTTGGC ACCAATGATT
    GTTCATGGCA TCAAGACTCA GGGTGCTCGT CAGAAATTTT CCAGCCTTTA TATCTCTCAA
    TTTATCATCA TGTATAGCCT GGATGGGAAG AAGTGGCTGA GTTATCAAGG AAATTCCACT
    GGAACCTTAA TGGTTTTCTT TGGCAATGTG GACTCATCTG GGATTAAGCA TAATAGTTTT
    AATCCTCCAA TTATTGCTCG ATATATCCGT TTGCACCCCA CTCATTCTAG CATCCGTAGT
    ACTCTTCGCA TGGAGTTGAT GGGCTGTGAT TTAAACAGTT GCAGCATACC ATTGGGAATG
    GAAAGTAAAG TAATATCAGA TACACAAATC ACTGCCTCAT CCTACTTCAC CAACATGTTT
    GCTACTTGGT CTCCTTCACA AGCTCGACTT CACCTCCAGG GAAGGACTAA TGCCTGGCGA
    CCTCAGGTGA ATGATCCAAA ACAATGGTTG CAAGTGGACT TACAAAAGAC AATGAAAGTC
    ACTGGAATAA TAACCCAGGG AGTGAAATCT CTCTTTACCA GCATGTTTGT GAAAGAGTTC
    CTTATTTCCA GCAGTCAAGA TGGCCATCAC TGGACTCAAA TTTTATACAA TGGCAAGGTA
    AAGGTTTTTC AGGGGAATCA GGACTCATCC ACACCTATGA TGAATTCTCT AGACCCACCA
    TTACTCACTC GCTATCTTCG AATTCACCCC CAGATCTGGG AGCACCAAAT TGCTCTGAGG
    CTTGAGATTC TAGGATGTGA GGCCCAGCAG CAATACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu56130
    Clone ID Related Accession (Same CDS sequence) XM_006527792.3 , XM_006527791.3 , XM_006527791.4 , XM_006527792.4
    Accession Version XM_006527791.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6801bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product coagulation factor VIII isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000086.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006527791.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    ATGTCAACAT CTTTTCCATT CAACACCTCC ATCATGTATA AAAAGACTGT GTTTGTAGAG 
    TACAAGGACC AGCTTTTCAA CATTGCCAAG CCCAGGCCAC CCTGGATGGG TTTGCTAGGT
    CCTACCATTT GGACTGAGGT TCATGACACA GTGGTCATTA CACTTAAAAA CATGGCTTCT
    CATCCTGTCA GTCTTCATGC TGTTGGTGTG TCCTACTGGA AAGCTTCTGA GGGAGATGAA
    TATGAAGATC AGACAAGCCA AATGGAGAAG GAAGATGATA AAGTTTTCCC TGGTGAAAGT
    CATACTTATG TTTGGCAAGT CCTGAAAGAG AATGGTCCAA TGGCCTCTGA CCCTCCATGT
    CTCACTTACT CATATATGTC TCATGTGGAT CTGGTGAAAG ATTTGAATTC AGGCCTCATT
    GGAGCTCTGC TAGTATGTAA AGAAGGCAGT CTCTCCAAAG AAAGAACACA GATGTTGTAC
    CAATTTGTAC TGCTTTTTGC TGTATTTGAT GAAGGGAAGA GCTGGCACTC AGAAACAAAC
    GACTCTTATA CACAGTCTAT GGATTCTGCA TCTGCTAGAG ACTGGCCTAA AATGCACACA
    GTCAATGGCT ATGTAAACAG GTCTCTTCCA GGTCTGATTG GATGCCATAG GAAATCAGTC
    TACTGGCACG TGATTGGAAT GGGCACCACT CCTGAAATAC ACTCAATATT CCTCGAAGGT
    CACACATTTT TTGTGAGGAA CCACCGTCAA GCTTCATTGG AGATATCACC AATAACTTTC
    CTTACTGCTC AAACACTCTT GATAGATCTT GGGCAGTTCC TACTATTTTG TCATATCTCT
    TCCCATAAAC ATGATGGCAT GGAAGCTTAT GTCAAAGTAG ATAGCTGCCC TGAGGAATCC
    CAATGGCAAA AGAAAAATAA TAATGAGGAA ATGGAAGATT ATGATGATGA TCTTTATTCA
    GAAATGGATA TGTTCACATT GGATTATGAC AGCTCTCCTT TTATCCAAAT TCGCTCGGTT
    GCTAAAAAGT ACCCTAAAAC TTGGATACAT TATATTTCTG CTGAGGAGGA AGACTGGGAC
    TATGCACCTT CAGTTCCTAC CTCGGATAAT GGAAGTTATA AAAGCCAGTA TCTGAGCAAT
    GGTCCTCATC GGATTGGTAG GAAATATAAA AAAGTCAGAT TTATAGCATA CACAGATGAA
    ACCTTTAAGA CTCGTGAAAC TATTCAGCAT GAATCAGGAC TCTTGGGACC TTTACTTTAT
    GGAGAAGTTG GAGACACACT GTTGATTATT TTTAAGAATC AAGCAAGCCG ACCATATAAC
    ATTTACCCTC ATGGAATCAC TGATGTCAGT CCTCTACATG CAAGGAGATT GCCAAGAGGT
    ATAAAGCACG TGAAGGATTT GCCAATTCAT CCAGGAGAGA TATTCAAGTA CAAGTGGACA
    GTTACAGTAG AAGATGGACC AACTAAATCA GATCCACGGT GCCTGACCCG CTATTATTCA
    AGTTTCATTA ACCCTGAGAG AGATCTAGCT TCAGGACTGA TTGGCCCTCT TCTCATCTGC
    TACAAAGAAT CTGTAGATCA AAGGGGAAAC CAGATGATGT CAGACAAAAG AAATGTCATC
    CTGTTTTCTA TATTTGATGA GAACCAAAGC TGGTACATCA CAGAGAACAT GCAACGCTTC
    CTCCCCAATG CAGCTAAAAC ACAGCCCCAG GACCCTGGGT TCCAGGCCTC CAACATCATG
    CACAGCATCA ATGGCTATGT TTTTGATAGC TTGGAGTTGA CAGTTTGTTT GCATGAGGTG
    GCATACTGGC ACATTCTCAG TGTTGGAGCA CAGACAGACT TCTTATCTAT CTTCTTCTCT
    GGATATACTT TCAAACACAA AATGGTCTAT GAAGATACAC TTACCCTGTT CCCATTCTCA
    GGAGAAACTG TCTTTATGTC GATGGAAAAC CCAGGTCTAT GGGTCTTGGG GTGTCATAAT
    TCAGACTTTC GGAAGAGAGG TATGACAGCA TTGCTGAAAG TTTCTAGTTG TGACAAGAGC
    ACTAGTGATT ATTATGAAGA AATATATGAA GATATTCCAA CACAGTTGGT GAATGAGAAC
    AATGTCATTG ATCCCAGAAG CTTCTTCCAG AATACAAATC ATCCTAATAC TAGGAAAAAG
    AAATTCAAAG ATTCCACAAT TCCAAAAAAT GATATGGAGA AGATTGAGCC TCAGTTTGAA
    GAGATAGCAG AGATGCTTAA AGTACAGAGT GTCTCAGTTA GTGACATGTT GATGCTCTTG
    GGACAGAGTC ATCCTACTCC ACATGGCTTA TTTTTATCAG ATGGCCAAGA AGCCATCTAT
    GAGGCTATTC ATGATGATCA TTCACCAAAT GCAATAGACA GCAATGAAGG CCCATCTAAA
    GTGACCCAAC TCAGGCCAGA ATCCCATCAC AGTGAGAAAA TAGTATTTAC TCCTCAGCCC
    GGCCTCCAGT TAAGATCCAA TAAAAGTTTG GAGACAACTA TAGAAGTAAA GTGGAAGAAA
    CTTGGTTTGC AAGTTTCTAG TTTGCCAAGT AATCTAATGA CTACAACAAT TCTGTCAGAC
    AATTTGAAAG CAACTTTTGA AAAGACAGAT TCTTCAGGAT TTCCAGATAT GCCAGTTCAC
    TCTAGTAGTA AATTAAGTAC TACTGCATTT GGTAAGAAAG CATATTCCCT TGTTGGGTCT
    CATGTACCTT TAAACGTGAG TGAAGAAAAT AGTGATTCCA ACATATTGGA TTCAACTTTA
    ATGTATAGTC AAGAAAGTTT ACCAAGAGAT AATATATTAT CAATGGAGAA TGATAGATTA
    CTCAGAGAGA AGAGGTTTCA TGGAATTGCT TTATTGACCA AAGATAATAC TTTATTCAAA
    GACAATGTCT CCTTAATGAA AACAAACAAA ACATATAATC ATTCAACAAC TAATGAAAAA
    CTACACACTG AGAGCCCAAC ATCAATTGAG AATAGTACAA CAGACTTGCA AGATGCCATA
    TTAAAGGTCA ATAGTGAGAT TCAAGAAGTA ACAGCTTTGA TTCATGATGG AACACTTTTA
    GGCAAAAATT CTACATATTT GAGACTAAAC CATATGCTAA ATAGAACTAC CTCAACAAAA
    AATAAAGACA TATTTCATAG AAAAGATGAA GATCCTATTC CACAAGATGA AGAGAATACA
    ATCATGCCAT TTTCCAAGAT GTTGTTCTTG TCAGAATCTT CAAATTGGTT TAAAAAGACC
    AATGGAAATA ATTCCTTGAA CTCTGAGCAA GAACATAGTC CAAAGCAATT AGTATATTTA
    ATGTTTAAAA AATATGTAAA AAATCAAAGT TTCTTGTCAG AGAAAAATAA AGTCACAGTA
    GAACAGGATG GATTTACAAA GAACATAGGA CTTAAAGACA TGGCTTTTCC ACATAATATG
    AGCATATTTC TTACCACTTT GTCTAACGTA CATGAAAATG GTAGGCACAA TCAAGAAAAA
    AATATTCAGG AAGAGATAGA GAAGGAAGCA CTAATTGAAG AGAAAGTAGT TTTGCCCCAG
    GTGCACGAAG CAACTGGCTC TAAGAATTTC TTGAAAGACA TATTGATACT AGGCACTAGG
    CAAAATATAA GTTTATATGA AGTACATGTA CCAGTACTTC AAAACATCAC ATCAATAAAC
    AATTCAACAA ATACAGTACA GATTCACATG GAGCATTTCT TTAAAAGAAG GAAGGACAAG
    GAAACAAATT CAGAAGGCTT GGTAAATAAA ACCAGAGAAA TGGTAAAAAA CTATCCAAGC
    CAGAAGAATA TTACTACTCA ACGTAGTAAA CGGGCTTTGG GACAATTCAG ACTGTCAACT
    CAATGGCTTA AAACCATAAA CTGTTCAACA CAGTGTATCA TTAAACAGAT AGACCACAGC
    AAGGAAATGA AAAAGTTCAT TACTAAATCT TCCTTATCAG ATTCTTCTGT GATTAAAAGC
    ACCACTCAGA CAAATAGTTC TGACTCACAC ATTGTAAAAA CATCAGCATT TCCACCAATA
    GATCTCAAAA GGAGTCCATT CCAAAACAAA TTTTCTCATG TTCAAGCATC ATCCTACATT
    TATGACTTTA AGACAAAAAG TTCAAGAATT CAAGAAAGCA ATAATTTCTT AAAAGAAACC
    AAAATAAATA ACCCTTCTTT AGCCATTCTA CCATGGAATA TGTTCATAGA TCAAGGAAAA
    TTTACCTCCC CAGGGAAAAG TAACACAAAC TCAGTCACAT ATAAGAAACG TGAGAACATT
    ATTTTCTTGA AACCAACTTT GCCTGAAGAA TCTGGCAAAA TTGAATTGCT TCCTCAAGTT
    TCCATTCAAG AGGAAGAAAT TTTACCTACA GAAACTAGCC ATGGATCTCC TGGACACTTG
    AATCTCATGA AAGAGGTCTT TCTTCAGAAA ATACAGGGGC CTACTAAATG GAATAAAGCA
    AAGAGGCATG GAGAAAGTAT AAAAGGTAAA ACAGAGAGCT CTAAAAATAC TCGCTCAAAA
    CTGCTAAATC ATCATGCTTG GGATTATCAT TATGCTGCAC AGATACCAAA AGATATGTGG
    AAATCCAAAG AGAAGTCACC AGAAATTATA TCCATTAAGC AAGAGGACAC CATTTTGTCT
    CTGAGGCCTC ATGGAAACAG TCATTCAATA GGGGCAAATG AGAAACAAAA TTGGCCTCAA
    AGAGAAACCA CTTGGGTAAA GCAAGGCCAA ACTCAAAGGA CATGCTCTCA AATCCCACCA
    GTGTTGAAAC GACATCAAAG GGAACTTAGT GCTTTTCAAT CAGAACAAGA AGCAACTGAC
    TATGATGATG CCATCACCAT TGAAACAATC GAGGATTTTG ACATTTACAG TGAGGACATA
    AAGCAAGGTC CCCGCAGCTT TCAACAGAAA ACAAGGCACT ATTTTATTGC AGCTGTGGAA
    CGACTCTGGG ACTATGGGAT GAGTACATCT CATGTTCTAC GAAATAGGTA TCAAAGTGAC
    AATGTACCTC AGTTCAAGAA AGTAGTTTTC CAGGAATTTA CTGATGGCTC CTTTAGTCAG
    CCCTTATATC GTGGAGAATT AAATGAACAC CTGGGGTTGT TGGGCCCATA TATAAGAGCA
    GAAGTTGAAG ACAACATTAT GGTAACTTTC AAAAACCAGG CCTCCCGTCC CTACTCCTTC
    TATTCTAGCC TCATTTCTTA TAAAGAAGAT CAGAGAGGAG AAGAACCTAG AAGAAACTTT
    GTCAAGCCTA ATGAAACCAA AATTTATTTT TGGAAAGTAC AACATCATAT GGCACCCACA
    GAAGATGAGT TTGACTGCAA GGCCTGGGCT TATTTCTCTG ATGTTGATCT TGAAAGAGAT
    ATGCACTCGG GATTAATTGG ACCCCTTCTG ATTTGCCACG CGAACACACT GAATCCTGCT
    CATGGGAGAC AAGTGTCAGT ACAGGAATTT GCTCTGCTTT TCACTATCTT TGATGAGACC
    AAGAGCTGGT ACTTCACTGA AAACGTGAAA AGGAACTGCA AGACACCCTG CAATTTCCAG
    ATGGAAGACC CCACTTTGAA AGAGAATTAT CGCTTCCATG CAATCAATGG TTATGTAATG
    GATACCCTAC CAGGCTTAGT AATGGCTCAA GATCAAAGGA TTCGATGGTA TCTTCTCAGC
    ATGGGCAACA ATGAGAACAT CCAATCTATT CATTTCAGTG GACATGTTTT CACTGTACGG
    AAAAAAGAGG AGTATAAAAT GGCAGTGTAC AACCTCTACC CAGGTGTTTT TGAGACTCTG
    GAAATGATAC CATCCAGAGC TGGAATATGG CGAGTAGAAT GCCTTATTGG CGAGCACTTA
    CAGGCTGGGA TGAGCACTCT TTTTCTGGTG TACAGCAAGC AGTGTCAGAT TCCTCTTGGA
    ATGGCTTCTG GAAGCATCCG TGATTTCCAG ATTACAGCTT CAGGACATTA TGGACAGTGG
    GCCCCAAACC TGGCAAGACT TCATTATTCC GGATCAATCA ATGCCTGGAG TACCAAGGAG
    CCCTTTTCTT GGATCAAGGT AGATCTGTTG GCACCAATGA TTGTTCATGG CATCAAGACT
    CAGGGTGCTC GTCAGAAATT TTCCAGCCTT TATATCTCTC AATTTATCAT CATGTATAGC
    CTGGATGGGA AGAAGTGGCT GAGTTATCAA GGAAATTCCA CTGGAACCTT AATGGTTTTC
    TTTGGCAATG TGGACTCATC TGGGATTAAG CATAATAGTT TTAATCCTCC AATTATTGCT
    CGATATATCC GTTTGCACCC CACTCATTCT AGCATCCGTA GTACTCTTCG CATGGAGTTG
    ATGGGCTGTG ATTTAAACAG TTGCAGCATA CCATTGGGAA TGGAAAGTAA AGTAATATCA
    GATACACAAA TCACTGCCTC ATCCTACTTC ACCAACATGT TTGCTACTTG GTCTCCTTCA
    CAAGCTCGAC TTCACCTCCA GGGAAGGACT AATGCCTGGC GACCTCAGGT GAATGATCCA
    AAACAATGGT TGCAAGTGGA CTTACAAAAG ACAATGAAAG TCACTGGAAT AATAACCCAG
    GGAGTGAAAT CTCTCTTTAC CAGCATGTTT GTGAAAGAGT TCCTTATTTC CAGCAGTCAA
    GATGGCCATC ACTGGACTCA AATTTTATAC AATGGCAAGG TAAAGGTTTT TCAGGGGAAT
    CAGGACTCAT CCACACCTAT GATGAATTCT CTAGACCCAC CATTACTCAC TCGCTATCTT
    CGAATTCACC CCCAGATCTG GGAGCACCAA ATTGCTCTGA GGCTTGAGAT TCTAGGATGT
    GAGGCCCAGC AGCAATACTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006527854.1
    CDS187..6987
    Translation

    Target ORF information:

    RefSeq Version XM_006527791.4
    Organism Mus musculus(house mouse)
    Definition Mus musculus coagulation factor VIII (F8), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006527791.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
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    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    ATGTCAACAT CTTTTCCATT CAACACCTCC ATCATGTATA AAAAGACTGT GTTTGTAGAG 
    TACAAGGACC AGCTTTTCAA CATTGCCAAG CCCAGGCCAC CCTGGATGGG TTTGCTAGGT
    CCTACCATTT GGACTGAGGT TCATGACACA GTGGTCATTA CACTTAAAAA CATGGCTTCT
    CATCCTGTCA GTCTTCATGC TGTTGGTGTG TCCTACTGGA AAGCTTCTGA GGGAGATGAA
    TATGAAGATC AGACAAGCCA AATGGAGAAG GAAGATGATA AAGTTTTCCC TGGTGAAAGT
    CATACTTATG TTTGGCAAGT CCTGAAAGAG AATGGTCCAA TGGCCTCTGA CCCTCCATGT
    CTCACTTACT CATATATGTC TCATGTGGAT CTGGTGAAAG ATTTGAATTC AGGCCTCATT
    GGAGCTCTGC TAGTATGTAA AGAAGGCAGT CTCTCCAAAG AAAGAACACA GATGTTGTAC
    CAATTTGTAC TGCTTTTTGC TGTATTTGAT GAAGGGAAGA GCTGGCACTC AGAAACAAAC
    GACTCTTATA CACAGTCTAT GGATTCTGCA TCTGCTAGAG ACTGGCCTAA AATGCACACA
    GTCAATGGCT ATGTAAACAG GTCTCTTCCA GGTCTGATTG GATGCCATAG GAAATCAGTC
    TACTGGCACG TGATTGGAAT GGGCACCACT CCTGAAATAC ACTCAATATT CCTCGAAGGT
    CACACATTTT TTGTGAGGAA CCACCGTCAA GCTTCATTGG AGATATCACC AATAACTTTC
    CTTACTGCTC AAACACTCTT GATAGATCTT GGGCAGTTCC TACTATTTTG TCATATCTCT
    TCCCATAAAC ATGATGGCAT GGAAGCTTAT GTCAAAGTAG ATAGCTGCCC TGAGGAATCC
    CAATGGCAAA AGAAAAATAA TAATGAGGAA ATGGAAGATT ATGATGATGA TCTTTATTCA
    GAAATGGATA TGTTCACATT GGATTATGAC AGCTCTCCTT TTATCCAAAT TCGCTCGGTT
    GCTAAAAAGT ACCCTAAAAC TTGGATACAT TATATTTCTG CTGAGGAGGA AGACTGGGAC
    TATGCACCTT CAGTTCCTAC CTCGGATAAT GGAAGTTATA AAAGCCAGTA TCTGAGCAAT
    GGTCCTCATC GGATTGGTAG GAAATATAAA AAAGTCAGAT TTATAGCATA CACAGATGAA
    ACCTTTAAGA CTCGTGAAAC TATTCAGCAT GAATCAGGAC TCTTGGGACC TTTACTTTAT
    GGAGAAGTTG GAGACACACT GTTGATTATT TTTAAGAATC AAGCAAGCCG ACCATATAAC
    ATTTACCCTC ATGGAATCAC TGATGTCAGT CCTCTACATG CAAGGAGATT GCCAAGAGGT
    ATAAAGCACG TGAAGGATTT GCCAATTCAT CCAGGAGAGA TATTCAAGTA CAAGTGGACA
    GTTACAGTAG AAGATGGACC AACTAAATCA GATCCACGGT GCCTGACCCG CTATTATTCA
    AGTTTCATTA ACCCTGAGAG AGATCTAGCT TCAGGACTGA TTGGCCCTCT TCTCATCTGC
    TACAAAGAAT CTGTAGATCA AAGGGGAAAC CAGATGATGT CAGACAAAAG AAATGTCATC
    CTGTTTTCTA TATTTGATGA GAACCAAAGC TGGTACATCA CAGAGAACAT GCAACGCTTC
    CTCCCCAATG CAGCTAAAAC ACAGCCCCAG GACCCTGGGT TCCAGGCCTC CAACATCATG
    CACAGCATCA ATGGCTATGT TTTTGATAGC TTGGAGTTGA CAGTTTGTTT GCATGAGGTG
    GCATACTGGC ACATTCTCAG TGTTGGAGCA CAGACAGACT TCTTATCTAT CTTCTTCTCT
    GGATATACTT TCAAACACAA AATGGTCTAT GAAGATACAC TTACCCTGTT CCCATTCTCA
    GGAGAAACTG TCTTTATGTC GATGGAAAAC CCAGGTCTAT GGGTCTTGGG GTGTCATAAT
    TCAGACTTTC GGAAGAGAGG TATGACAGCA TTGCTGAAAG TTTCTAGTTG TGACAAGAGC
    ACTAGTGATT ATTATGAAGA AATATATGAA GATATTCCAA CACAGTTGGT GAATGAGAAC
    AATGTCATTG ATCCCAGAAG CTTCTTCCAG AATACAAATC ATCCTAATAC TAGGAAAAAG
    AAATTCAAAG ATTCCACAAT TCCAAAAAAT GATATGGAGA AGATTGAGCC TCAGTTTGAA
    GAGATAGCAG AGATGCTTAA AGTACAGAGT GTCTCAGTTA GTGACATGTT GATGCTCTTG
    GGACAGAGTC ATCCTACTCC ACATGGCTTA TTTTTATCAG ATGGCCAAGA AGCCATCTAT
    GAGGCTATTC ATGATGATCA TTCACCAAAT GCAATAGACA GCAATGAAGG CCCATCTAAA
    GTGACCCAAC TCAGGCCAGA ATCCCATCAC AGTGAGAAAA TAGTATTTAC TCCTCAGCCC
    GGCCTCCAGT TAAGATCCAA TAAAAGTTTG GAGACAACTA TAGAAGTAAA GTGGAAGAAA
    CTTGGTTTGC AAGTTTCTAG TTTGCCAAGT AATCTAATGA CTACAACAAT TCTGTCAGAC
    AATTTGAAAG CAACTTTTGA AAAGACAGAT TCTTCAGGAT TTCCAGATAT GCCAGTTCAC
    TCTAGTAGTA AATTAAGTAC TACTGCATTT GGTAAGAAAG CATATTCCCT TGTTGGGTCT
    CATGTACCTT TAAACGTGAG TGAAGAAAAT AGTGATTCCA ACATATTGGA TTCAACTTTA
    ATGTATAGTC AAGAAAGTTT ACCAAGAGAT AATATATTAT CAATGGAGAA TGATAGATTA
    CTCAGAGAGA AGAGGTTTCA TGGAATTGCT TTATTGACCA AAGATAATAC TTTATTCAAA
    GACAATGTCT CCTTAATGAA AACAAACAAA ACATATAATC ATTCAACAAC TAATGAAAAA
    CTACACACTG AGAGCCCAAC ATCAATTGAG AATAGTACAA CAGACTTGCA AGATGCCATA
    TTAAAGGTCA ATAGTGAGAT TCAAGAAGTA ACAGCTTTGA TTCATGATGG AACACTTTTA
    GGCAAAAATT CTACATATTT GAGACTAAAC CATATGCTAA ATAGAACTAC CTCAACAAAA
    AATAAAGACA TATTTCATAG AAAAGATGAA GATCCTATTC CACAAGATGA AGAGAATACA
    ATCATGCCAT TTTCCAAGAT GTTGTTCTTG TCAGAATCTT CAAATTGGTT TAAAAAGACC
    AATGGAAATA ATTCCTTGAA CTCTGAGCAA GAACATAGTC CAAAGCAATT AGTATATTTA
    ATGTTTAAAA AATATGTAAA AAATCAAAGT TTCTTGTCAG AGAAAAATAA AGTCACAGTA
    GAACAGGATG GATTTACAAA GAACATAGGA CTTAAAGACA TGGCTTTTCC ACATAATATG
    AGCATATTTC TTACCACTTT GTCTAACGTA CATGAAAATG GTAGGCACAA TCAAGAAAAA
    AATATTCAGG AAGAGATAGA GAAGGAAGCA CTAATTGAAG AGAAAGTAGT TTTGCCCCAG
    GTGCACGAAG CAACTGGCTC TAAGAATTTC TTGAAAGACA TATTGATACT AGGCACTAGG
    CAAAATATAA GTTTATATGA AGTACATGTA CCAGTACTTC AAAACATCAC ATCAATAAAC
    AATTCAACAA ATACAGTACA GATTCACATG GAGCATTTCT TTAAAAGAAG GAAGGACAAG
    GAAACAAATT CAGAAGGCTT GGTAAATAAA ACCAGAGAAA TGGTAAAAAA CTATCCAAGC
    CAGAAGAATA TTACTACTCA ACGTAGTAAA CGGGCTTTGG GACAATTCAG ACTGTCAACT
    CAATGGCTTA AAACCATAAA CTGTTCAACA CAGTGTATCA TTAAACAGAT AGACCACAGC
    AAGGAAATGA AAAAGTTCAT TACTAAATCT TCCTTATCAG ATTCTTCTGT GATTAAAAGC
    ACCACTCAGA CAAATAGTTC TGACTCACAC ATTGTAAAAA CATCAGCATT TCCACCAATA
    GATCTCAAAA GGAGTCCATT CCAAAACAAA TTTTCTCATG TTCAAGCATC ATCCTACATT
    TATGACTTTA AGACAAAAAG TTCAAGAATT CAAGAAAGCA ATAATTTCTT AAAAGAAACC
    AAAATAAATA ACCCTTCTTT AGCCATTCTA CCATGGAATA TGTTCATAGA TCAAGGAAAA
    TTTACCTCCC CAGGGAAAAG TAACACAAAC TCAGTCACAT ATAAGAAACG TGAGAACATT
    ATTTTCTTGA AACCAACTTT GCCTGAAGAA TCTGGCAAAA TTGAATTGCT TCCTCAAGTT
    TCCATTCAAG AGGAAGAAAT TTTACCTACA GAAACTAGCC ATGGATCTCC TGGACACTTG
    AATCTCATGA AAGAGGTCTT TCTTCAGAAA ATACAGGGGC CTACTAAATG GAATAAAGCA
    AAGAGGCATG GAGAAAGTAT AAAAGGTAAA ACAGAGAGCT CTAAAAATAC TCGCTCAAAA
    CTGCTAAATC ATCATGCTTG GGATTATCAT TATGCTGCAC AGATACCAAA AGATATGTGG
    AAATCCAAAG AGAAGTCACC AGAAATTATA TCCATTAAGC AAGAGGACAC CATTTTGTCT
    CTGAGGCCTC ATGGAAACAG TCATTCAATA GGGGCAAATG AGAAACAAAA TTGGCCTCAA
    AGAGAAACCA CTTGGGTAAA GCAAGGCCAA ACTCAAAGGA CATGCTCTCA AATCCCACCA
    GTGTTGAAAC GACATCAAAG GGAACTTAGT GCTTTTCAAT CAGAACAAGA AGCAACTGAC
    TATGATGATG CCATCACCAT TGAAACAATC GAGGATTTTG ACATTTACAG TGAGGACATA
    AAGCAAGGTC CCCGCAGCTT TCAACAGAAA ACAAGGCACT ATTTTATTGC AGCTGTGGAA
    CGACTCTGGG ACTATGGGAT GAGTACATCT CATGTTCTAC GAAATAGGTA TCAAAGTGAC
    AATGTACCTC AGTTCAAGAA AGTAGTTTTC CAGGAATTTA CTGATGGCTC CTTTAGTCAG
    CCCTTATATC GTGGAGAATT AAATGAACAC CTGGGGTTGT TGGGCCCATA TATAAGAGCA
    GAAGTTGAAG ACAACATTAT GGTAACTTTC AAAAACCAGG CCTCCCGTCC CTACTCCTTC
    TATTCTAGCC TCATTTCTTA TAAAGAAGAT CAGAGAGGAG AAGAACCTAG AAGAAACTTT
    GTCAAGCCTA ATGAAACCAA AATTTATTTT TGGAAAGTAC AACATCATAT GGCACCCACA
    GAAGATGAGT TTGACTGCAA GGCCTGGGCT TATTTCTCTG ATGTTGATCT TGAAAGAGAT
    ATGCACTCGG GATTAATTGG ACCCCTTCTG ATTTGCCACG CGAACACACT GAATCCTGCT
    CATGGGAGAC AAGTGTCAGT ACAGGAATTT GCTCTGCTTT TCACTATCTT TGATGAGACC
    AAGAGCTGGT ACTTCACTGA AAACGTGAAA AGGAACTGCA AGACACCCTG CAATTTCCAG
    ATGGAAGACC CCACTTTGAA AGAGAATTAT CGCTTCCATG CAATCAATGG TTATGTAATG
    GATACCCTAC CAGGCTTAGT AATGGCTCAA GATCAAAGGA TTCGATGGTA TCTTCTCAGC
    ATGGGCAACA ATGAGAACAT CCAATCTATT CATTTCAGTG GACATGTTTT CACTGTACGG
    AAAAAAGAGG AGTATAAAAT GGCAGTGTAC AACCTCTACC CAGGTGTTTT TGAGACTCTG
    GAAATGATAC CATCCAGAGC TGGAATATGG CGAGTAGAAT GCCTTATTGG CGAGCACTTA
    CAGGCTGGGA TGAGCACTCT TTTTCTGGTG TACAGCAAGC AGTGTCAGAT TCCTCTTGGA
    ATGGCTTCTG GAAGCATCCG TGATTTCCAG ATTACAGCTT CAGGACATTA TGGACAGTGG
    GCCCCAAACC TGGCAAGACT TCATTATTCC GGATCAATCA ATGCCTGGAG TACCAAGGAG
    CCCTTTTCTT GGATCAAGGT AGATCTGTTG GCACCAATGA TTGTTCATGG CATCAAGACT
    CAGGGTGCTC GTCAGAAATT TTCCAGCCTT TATATCTCTC AATTTATCAT CATGTATAGC
    CTGGATGGGA AGAAGTGGCT GAGTTATCAA GGAAATTCCA CTGGAACCTT AATGGTTTTC
    TTTGGCAATG TGGACTCATC TGGGATTAAG CATAATAGTT TTAATCCTCC AATTATTGCT
    CGATATATCC GTTTGCACCC CACTCATTCT AGCATCCGTA GTACTCTTCG CATGGAGTTG
    ATGGGCTGTG ATTTAAACAG TTGCAGCATA CCATTGGGAA TGGAAAGTAA AGTAATATCA
    GATACACAAA TCACTGCCTC ATCCTACTTC ACCAACATGT TTGCTACTTG GTCTCCTTCA
    CAAGCTCGAC TTCACCTCCA GGGAAGGACT AATGCCTGGC GACCTCAGGT GAATGATCCA
    AAACAATGGT TGCAAGTGGA CTTACAAAAG ACAATGAAAG TCACTGGAAT AATAACCCAG
    GGAGTGAAAT CTCTCTTTAC CAGCATGTTT GTGAAAGAGT TCCTTATTTC CAGCAGTCAA
    GATGGCCATC ACTGGACTCA AATTTTATAC AATGGCAAGG TAAAGGTTTT TCAGGGGAAT
    CAGGACTCAT CCACACCTAT GATGAATTCT CTAGACCCAC CATTACTCAC TCGCTATCTT
    CGAATTCACC CCCAGATCTG GGAGCACCAA ATTGCTCTGA GGCTTGAGAT TCTAGGATGT
    GAGGCCCAGC AGCAATACTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu56130
    Clone ID Related Accession (Same CDS sequence) XM_006527792.3 , XM_006527791.3 , XM_006527791.4 , XM_006527792.4
    Accession Version XM_006527792.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6801bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product coagulation factor VIII isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000086.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006527792.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

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    181
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    301
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    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    ATGTCAACAT CTTTTCCATT CAACACCTCC ATCATGTATA AAAAGACTGT GTTTGTAGAG 
    TACAAGGACC AGCTTTTCAA CATTGCCAAG CCCAGGCCAC CCTGGATGGG TTTGCTAGGT
    CCTACCATTT GGACTGAGGT TCATGACACA GTGGTCATTA CACTTAAAAA CATGGCTTCT
    CATCCTGTCA GTCTTCATGC TGTTGGTGTG TCCTACTGGA AAGCTTCTGA GGGAGATGAA
    TATGAAGATC AGACAAGCCA AATGGAGAAG GAAGATGATA AAGTTTTCCC TGGTGAAAGT
    CATACTTATG TTTGGCAAGT CCTGAAAGAG AATGGTCCAA TGGCCTCTGA CCCTCCATGT
    CTCACTTACT CATATATGTC TCATGTGGAT CTGGTGAAAG ATTTGAATTC AGGCCTCATT
    GGAGCTCTGC TAGTATGTAA AGAAGGCAGT CTCTCCAAAG AAAGAACACA GATGTTGTAC
    CAATTTGTAC TGCTTTTTGC TGTATTTGAT GAAGGGAAGA GCTGGCACTC AGAAACAAAC
    GACTCTTATA CACAGTCTAT GGATTCTGCA TCTGCTAGAG ACTGGCCTAA AATGCACACA
    GTCAATGGCT ATGTAAACAG GTCTCTTCCA GGTCTGATTG GATGCCATAG GAAATCAGTC
    TACTGGCACG TGATTGGAAT GGGCACCACT CCTGAAATAC ACTCAATATT CCTCGAAGGT
    CACACATTTT TTGTGAGGAA CCACCGTCAA GCTTCATTGG AGATATCACC AATAACTTTC
    CTTACTGCTC AAACACTCTT GATAGATCTT GGGCAGTTCC TACTATTTTG TCATATCTCT
    TCCCATAAAC ATGATGGCAT GGAAGCTTAT GTCAAAGTAG ATAGCTGCCC TGAGGAATCC
    CAATGGCAAA AGAAAAATAA TAATGAGGAA ATGGAAGATT ATGATGATGA TCTTTATTCA
    GAAATGGATA TGTTCACATT GGATTATGAC AGCTCTCCTT TTATCCAAAT TCGCTCGGTT
    GCTAAAAAGT ACCCTAAAAC TTGGATACAT TATATTTCTG CTGAGGAGGA AGACTGGGAC
    TATGCACCTT CAGTTCCTAC CTCGGATAAT GGAAGTTATA AAAGCCAGTA TCTGAGCAAT
    GGTCCTCATC GGATTGGTAG GAAATATAAA AAAGTCAGAT TTATAGCATA CACAGATGAA
    ACCTTTAAGA CTCGTGAAAC TATTCAGCAT GAATCAGGAC TCTTGGGACC TTTACTTTAT
    GGAGAAGTTG GAGACACACT GTTGATTATT TTTAAGAATC AAGCAAGCCG ACCATATAAC
    ATTTACCCTC ATGGAATCAC TGATGTCAGT CCTCTACATG CAAGGAGATT GCCAAGAGGT
    ATAAAGCACG TGAAGGATTT GCCAATTCAT CCAGGAGAGA TATTCAAGTA CAAGTGGACA
    GTTACAGTAG AAGATGGACC AACTAAATCA GATCCACGGT GCCTGACCCG CTATTATTCA
    AGTTTCATTA ACCCTGAGAG AGATCTAGCT TCAGGACTGA TTGGCCCTCT TCTCATCTGC
    TACAAAGAAT CTGTAGATCA AAGGGGAAAC CAGATGATGT CAGACAAAAG AAATGTCATC
    CTGTTTTCTA TATTTGATGA GAACCAAAGC TGGTACATCA CAGAGAACAT GCAACGCTTC
    CTCCCCAATG CAGCTAAAAC ACAGCCCCAG GACCCTGGGT TCCAGGCCTC CAACATCATG
    CACAGCATCA ATGGCTATGT TTTTGATAGC TTGGAGTTGA CAGTTTGTTT GCATGAGGTG
    GCATACTGGC ACATTCTCAG TGTTGGAGCA CAGACAGACT TCTTATCTAT CTTCTTCTCT
    GGATATACTT TCAAACACAA AATGGTCTAT GAAGATACAC TTACCCTGTT CCCATTCTCA
    GGAGAAACTG TCTTTATGTC GATGGAAAAC CCAGGTCTAT GGGTCTTGGG GTGTCATAAT
    TCAGACTTTC GGAAGAGAGG TATGACAGCA TTGCTGAAAG TTTCTAGTTG TGACAAGAGC
    ACTAGTGATT ATTATGAAGA AATATATGAA GATATTCCAA CACAGTTGGT GAATGAGAAC
    AATGTCATTG ATCCCAGAAG CTTCTTCCAG AATACAAATC ATCCTAATAC TAGGAAAAAG
    AAATTCAAAG ATTCCACAAT TCCAAAAAAT GATATGGAGA AGATTGAGCC TCAGTTTGAA
    GAGATAGCAG AGATGCTTAA AGTACAGAGT GTCTCAGTTA GTGACATGTT GATGCTCTTG
    GGACAGAGTC ATCCTACTCC ACATGGCTTA TTTTTATCAG ATGGCCAAGA AGCCATCTAT
    GAGGCTATTC ATGATGATCA TTCACCAAAT GCAATAGACA GCAATGAAGG CCCATCTAAA
    GTGACCCAAC TCAGGCCAGA ATCCCATCAC AGTGAGAAAA TAGTATTTAC TCCTCAGCCC
    GGCCTCCAGT TAAGATCCAA TAAAAGTTTG GAGACAACTA TAGAAGTAAA GTGGAAGAAA
    CTTGGTTTGC AAGTTTCTAG TTTGCCAAGT AATCTAATGA CTACAACAAT TCTGTCAGAC
    AATTTGAAAG CAACTTTTGA AAAGACAGAT TCTTCAGGAT TTCCAGATAT GCCAGTTCAC
    TCTAGTAGTA AATTAAGTAC TACTGCATTT GGTAAGAAAG CATATTCCCT TGTTGGGTCT
    CATGTACCTT TAAACGTGAG TGAAGAAAAT AGTGATTCCA ACATATTGGA TTCAACTTTA
    ATGTATAGTC AAGAAAGTTT ACCAAGAGAT AATATATTAT CAATGGAGAA TGATAGATTA
    CTCAGAGAGA AGAGGTTTCA TGGAATTGCT TTATTGACCA AAGATAATAC TTTATTCAAA
    GACAATGTCT CCTTAATGAA AACAAACAAA ACATATAATC ATTCAACAAC TAATGAAAAA
    CTACACACTG AGAGCCCAAC ATCAATTGAG AATAGTACAA CAGACTTGCA AGATGCCATA
    TTAAAGGTCA ATAGTGAGAT TCAAGAAGTA ACAGCTTTGA TTCATGATGG AACACTTTTA
    GGCAAAAATT CTACATATTT GAGACTAAAC CATATGCTAA ATAGAACTAC CTCAACAAAA
    AATAAAGACA TATTTCATAG AAAAGATGAA GATCCTATTC CACAAGATGA AGAGAATACA
    ATCATGCCAT TTTCCAAGAT GTTGTTCTTG TCAGAATCTT CAAATTGGTT TAAAAAGACC
    AATGGAAATA ATTCCTTGAA CTCTGAGCAA GAACATAGTC CAAAGCAATT AGTATATTTA
    ATGTTTAAAA AATATGTAAA AAATCAAAGT TTCTTGTCAG AGAAAAATAA AGTCACAGTA
    GAACAGGATG GATTTACAAA GAACATAGGA CTTAAAGACA TGGCTTTTCC ACATAATATG
    AGCATATTTC TTACCACTTT GTCTAACGTA CATGAAAATG GTAGGCACAA TCAAGAAAAA
    AATATTCAGG AAGAGATAGA GAAGGAAGCA CTAATTGAAG AGAAAGTAGT TTTGCCCCAG
    GTGCACGAAG CAACTGGCTC TAAGAATTTC TTGAAAGACA TATTGATACT AGGCACTAGG
    CAAAATATAA GTTTATATGA AGTACATGTA CCAGTACTTC AAAACATCAC ATCAATAAAC
    AATTCAACAA ATACAGTACA GATTCACATG GAGCATTTCT TTAAAAGAAG GAAGGACAAG
    GAAACAAATT CAGAAGGCTT GGTAAATAAA ACCAGAGAAA TGGTAAAAAA CTATCCAAGC
    CAGAAGAATA TTACTACTCA ACGTAGTAAA CGGGCTTTGG GACAATTCAG ACTGTCAACT
    CAATGGCTTA AAACCATAAA CTGTTCAACA CAGTGTATCA TTAAACAGAT AGACCACAGC
    AAGGAAATGA AAAAGTTCAT TACTAAATCT TCCTTATCAG ATTCTTCTGT GATTAAAAGC
    ACCACTCAGA CAAATAGTTC TGACTCACAC ATTGTAAAAA CATCAGCATT TCCACCAATA
    GATCTCAAAA GGAGTCCATT CCAAAACAAA TTTTCTCATG TTCAAGCATC ATCCTACATT
    TATGACTTTA AGACAAAAAG TTCAAGAATT CAAGAAAGCA ATAATTTCTT AAAAGAAACC
    AAAATAAATA ACCCTTCTTT AGCCATTCTA CCATGGAATA TGTTCATAGA TCAAGGAAAA
    TTTACCTCCC CAGGGAAAAG TAACACAAAC TCAGTCACAT ATAAGAAACG TGAGAACATT
    ATTTTCTTGA AACCAACTTT GCCTGAAGAA TCTGGCAAAA TTGAATTGCT TCCTCAAGTT
    TCCATTCAAG AGGAAGAAAT TTTACCTACA GAAACTAGCC ATGGATCTCC TGGACACTTG
    AATCTCATGA AAGAGGTCTT TCTTCAGAAA ATACAGGGGC CTACTAAATG GAATAAAGCA
    AAGAGGCATG GAGAAAGTAT AAAAGGTAAA ACAGAGAGCT CTAAAAATAC TCGCTCAAAA
    CTGCTAAATC ATCATGCTTG GGATTATCAT TATGCTGCAC AGATACCAAA AGATATGTGG
    AAATCCAAAG AGAAGTCACC AGAAATTATA TCCATTAAGC AAGAGGACAC CATTTTGTCT
    CTGAGGCCTC ATGGAAACAG TCATTCAATA GGGGCAAATG AGAAACAAAA TTGGCCTCAA
    AGAGAAACCA CTTGGGTAAA GCAAGGCCAA ACTCAAAGGA CATGCTCTCA AATCCCACCA
    GTGTTGAAAC GACATCAAAG GGAACTTAGT GCTTTTCAAT CAGAACAAGA AGCAACTGAC
    TATGATGATG CCATCACCAT TGAAACAATC GAGGATTTTG ACATTTACAG TGAGGACATA
    AAGCAAGGTC CCCGCAGCTT TCAACAGAAA ACAAGGCACT ATTTTATTGC AGCTGTGGAA
    CGACTCTGGG ACTATGGGAT GAGTACATCT CATGTTCTAC GAAATAGGTA TCAAAGTGAC
    AATGTACCTC AGTTCAAGAA AGTAGTTTTC CAGGAATTTA CTGATGGCTC CTTTAGTCAG
    CCCTTATATC GTGGAGAATT AAATGAACAC CTGGGGTTGT TGGGCCCATA TATAAGAGCA
    GAAGTTGAAG ACAACATTAT GGTAACTTTC AAAAACCAGG CCTCCCGTCC CTACTCCTTC
    TATTCTAGCC TCATTTCTTA TAAAGAAGAT CAGAGAGGAG AAGAACCTAG AAGAAACTTT
    GTCAAGCCTA ATGAAACCAA AATTTATTTT TGGAAAGTAC AACATCATAT GGCACCCACA
    GAAGATGAGT TTGACTGCAA GGCCTGGGCT TATTTCTCTG ATGTTGATCT TGAAAGAGAT
    ATGCACTCGG GATTAATTGG ACCCCTTCTG ATTTGCCACG CGAACACACT GAATCCTGCT
    CATGGGAGAC AAGTGTCAGT ACAGGAATTT GCTCTGCTTT TCACTATCTT TGATGAGACC
    AAGAGCTGGT ACTTCACTGA AAACGTGAAA AGGAACTGCA AGACACCCTG CAATTTCCAG
    ATGGAAGACC CCACTTTGAA AGAGAATTAT CGCTTCCATG CAATCAATGG TTATGTAATG
    GATACCCTAC CAGGCTTAGT AATGGCTCAA GATCAAAGGA TTCGATGGTA TCTTCTCAGC
    ATGGGCAACA ATGAGAACAT CCAATCTATT CATTTCAGTG GACATGTTTT CACTGTACGG
    AAAAAAGAGG AGTATAAAAT GGCAGTGTAC AACCTCTACC CAGGTGTTTT TGAGACTCTG
    GAAATGATAC CATCCAGAGC TGGAATATGG CGAGTAGAAT GCCTTATTGG CGAGCACTTA
    CAGGCTGGGA TGAGCACTCT TTTTCTGGTG TACAGCAAGC AGTGTCAGAT TCCTCTTGGA
    ATGGCTTCTG GAAGCATCCG TGATTTCCAG ATTACAGCTT CAGGACATTA TGGACAGTGG
    GCCCCAAACC TGGCAAGACT TCATTATTCC GGATCAATCA ATGCCTGGAG TACCAAGGAG
    CCCTTTTCTT GGATCAAGGT AGATCTGTTG GCACCAATGA TTGTTCATGG CATCAAGACT
    CAGGGTGCTC GTCAGAAATT TTCCAGCCTT TATATCTCTC AATTTATCAT CATGTATAGC
    CTGGATGGGA AGAAGTGGCT GAGTTATCAA GGAAATTCCA CTGGAACCTT AATGGTTTTC
    TTTGGCAATG TGGACTCATC TGGGATTAAG CATAATAGTT TTAATCCTCC AATTATTGCT
    CGATATATCC GTTTGCACCC CACTCATTCT AGCATCCGTA GTACTCTTCG CATGGAGTTG
    ATGGGCTGTG ATTTAAACAG TTGCAGCATA CCATTGGGAA TGGAAAGTAA AGTAATATCA
    GATACACAAA TCACTGCCTC ATCCTACTTC ACCAACATGT TTGCTACTTG GTCTCCTTCA
    CAAGCTCGAC TTCACCTCCA GGGAAGGACT AATGCCTGGC GACCTCAGGT GAATGATCCA
    AAACAATGGT TGCAAGTGGA CTTACAAAAG ACAATGAAAG TCACTGGAAT AATAACCCAG
    GGAGTGAAAT CTCTCTTTAC CAGCATGTTT GTGAAAGAGT TCCTTATTTC CAGCAGTCAA
    GATGGCCATC ACTGGACTCA AATTTTATAC AATGGCAAGG TAAAGGTTTT TCAGGGGAAT
    CAGGACTCAT CCACACCTAT GATGAATTCT CTAGACCCAC CATTACTCAC TCGCTATCTT
    CGAATTCACC CCCAGATCTG GGAGCACCAA ATTGCTCTGA GGCTTGAGAT TCTAGGATGT
    GAGGCCCAGC AGCAATACTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006527855.1
    CDS340..7140
    Translation

    Target ORF information:

    RefSeq Version XM_006527792.4
    Organism Mus musculus(house mouse)
    Definition Mus musculus coagulation factor VIII (F8), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006527792.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    ATGTCAACAT CTTTTCCATT CAACACCTCC ATCATGTATA AAAAGACTGT GTTTGTAGAG 
    TACAAGGACC AGCTTTTCAA CATTGCCAAG CCCAGGCCAC CCTGGATGGG TTTGCTAGGT
    CCTACCATTT GGACTGAGGT TCATGACACA GTGGTCATTA CACTTAAAAA CATGGCTTCT
    CATCCTGTCA GTCTTCATGC TGTTGGTGTG TCCTACTGGA AAGCTTCTGA GGGAGATGAA
    TATGAAGATC AGACAAGCCA AATGGAGAAG GAAGATGATA AAGTTTTCCC TGGTGAAAGT
    CATACTTATG TTTGGCAAGT CCTGAAAGAG AATGGTCCAA TGGCCTCTGA CCCTCCATGT
    CTCACTTACT CATATATGTC TCATGTGGAT CTGGTGAAAG ATTTGAATTC AGGCCTCATT
    GGAGCTCTGC TAGTATGTAA AGAAGGCAGT CTCTCCAAAG AAAGAACACA GATGTTGTAC
    CAATTTGTAC TGCTTTTTGC TGTATTTGAT GAAGGGAAGA GCTGGCACTC AGAAACAAAC
    GACTCTTATA CACAGTCTAT GGATTCTGCA TCTGCTAGAG ACTGGCCTAA AATGCACACA
    GTCAATGGCT ATGTAAACAG GTCTCTTCCA GGTCTGATTG GATGCCATAG GAAATCAGTC
    TACTGGCACG TGATTGGAAT GGGCACCACT CCTGAAATAC ACTCAATATT CCTCGAAGGT
    CACACATTTT TTGTGAGGAA CCACCGTCAA GCTTCATTGG AGATATCACC AATAACTTTC
    CTTACTGCTC AAACACTCTT GATAGATCTT GGGCAGTTCC TACTATTTTG TCATATCTCT
    TCCCATAAAC ATGATGGCAT GGAAGCTTAT GTCAAAGTAG ATAGCTGCCC TGAGGAATCC
    CAATGGCAAA AGAAAAATAA TAATGAGGAA ATGGAAGATT ATGATGATGA TCTTTATTCA
    GAAATGGATA TGTTCACATT GGATTATGAC AGCTCTCCTT TTATCCAAAT TCGCTCGGTT
    GCTAAAAAGT ACCCTAAAAC TTGGATACAT TATATTTCTG CTGAGGAGGA AGACTGGGAC
    TATGCACCTT CAGTTCCTAC CTCGGATAAT GGAAGTTATA AAAGCCAGTA TCTGAGCAAT
    GGTCCTCATC GGATTGGTAG GAAATATAAA AAAGTCAGAT TTATAGCATA CACAGATGAA
    ACCTTTAAGA CTCGTGAAAC TATTCAGCAT GAATCAGGAC TCTTGGGACC TTTACTTTAT
    GGAGAAGTTG GAGACACACT GTTGATTATT TTTAAGAATC AAGCAAGCCG ACCATATAAC
    ATTTACCCTC ATGGAATCAC TGATGTCAGT CCTCTACATG CAAGGAGATT GCCAAGAGGT
    ATAAAGCACG TGAAGGATTT GCCAATTCAT CCAGGAGAGA TATTCAAGTA CAAGTGGACA
    GTTACAGTAG AAGATGGACC AACTAAATCA GATCCACGGT GCCTGACCCG CTATTATTCA
    AGTTTCATTA ACCCTGAGAG AGATCTAGCT TCAGGACTGA TTGGCCCTCT TCTCATCTGC
    TACAAAGAAT CTGTAGATCA AAGGGGAAAC CAGATGATGT CAGACAAAAG AAATGTCATC
    CTGTTTTCTA TATTTGATGA GAACCAAAGC TGGTACATCA CAGAGAACAT GCAACGCTTC
    CTCCCCAATG CAGCTAAAAC ACAGCCCCAG GACCCTGGGT TCCAGGCCTC CAACATCATG
    CACAGCATCA ATGGCTATGT TTTTGATAGC TTGGAGTTGA CAGTTTGTTT GCATGAGGTG
    GCATACTGGC ACATTCTCAG TGTTGGAGCA CAGACAGACT TCTTATCTAT CTTCTTCTCT
    GGATATACTT TCAAACACAA AATGGTCTAT GAAGATACAC TTACCCTGTT CCCATTCTCA
    GGAGAAACTG TCTTTATGTC GATGGAAAAC CCAGGTCTAT GGGTCTTGGG GTGTCATAAT
    TCAGACTTTC GGAAGAGAGG TATGACAGCA TTGCTGAAAG TTTCTAGTTG TGACAAGAGC
    ACTAGTGATT ATTATGAAGA AATATATGAA GATATTCCAA CACAGTTGGT GAATGAGAAC
    AATGTCATTG ATCCCAGAAG CTTCTTCCAG AATACAAATC ATCCTAATAC TAGGAAAAAG
    AAATTCAAAG ATTCCACAAT TCCAAAAAAT GATATGGAGA AGATTGAGCC TCAGTTTGAA
    GAGATAGCAG AGATGCTTAA AGTACAGAGT GTCTCAGTTA GTGACATGTT GATGCTCTTG
    GGACAGAGTC ATCCTACTCC ACATGGCTTA TTTTTATCAG ATGGCCAAGA AGCCATCTAT
    GAGGCTATTC ATGATGATCA TTCACCAAAT GCAATAGACA GCAATGAAGG CCCATCTAAA
    GTGACCCAAC TCAGGCCAGA ATCCCATCAC AGTGAGAAAA TAGTATTTAC TCCTCAGCCC
    GGCCTCCAGT TAAGATCCAA TAAAAGTTTG GAGACAACTA TAGAAGTAAA GTGGAAGAAA
    CTTGGTTTGC AAGTTTCTAG TTTGCCAAGT AATCTAATGA CTACAACAAT TCTGTCAGAC
    AATTTGAAAG CAACTTTTGA AAAGACAGAT TCTTCAGGAT TTCCAGATAT GCCAGTTCAC
    TCTAGTAGTA AATTAAGTAC TACTGCATTT GGTAAGAAAG CATATTCCCT TGTTGGGTCT
    CATGTACCTT TAAACGTGAG TGAAGAAAAT AGTGATTCCA ACATATTGGA TTCAACTTTA
    ATGTATAGTC AAGAAAGTTT ACCAAGAGAT AATATATTAT CAATGGAGAA TGATAGATTA
    CTCAGAGAGA AGAGGTTTCA TGGAATTGCT TTATTGACCA AAGATAATAC TTTATTCAAA
    GACAATGTCT CCTTAATGAA AACAAACAAA ACATATAATC ATTCAACAAC TAATGAAAAA
    CTACACACTG AGAGCCCAAC ATCAATTGAG AATAGTACAA CAGACTTGCA AGATGCCATA
    TTAAAGGTCA ATAGTGAGAT TCAAGAAGTA ACAGCTTTGA TTCATGATGG AACACTTTTA
    GGCAAAAATT CTACATATTT GAGACTAAAC CATATGCTAA ATAGAACTAC CTCAACAAAA
    AATAAAGACA TATTTCATAG AAAAGATGAA GATCCTATTC CACAAGATGA AGAGAATACA
    ATCATGCCAT TTTCCAAGAT GTTGTTCTTG TCAGAATCTT CAAATTGGTT TAAAAAGACC
    AATGGAAATA ATTCCTTGAA CTCTGAGCAA GAACATAGTC CAAAGCAATT AGTATATTTA
    ATGTTTAAAA AATATGTAAA AAATCAAAGT TTCTTGTCAG AGAAAAATAA AGTCACAGTA
    GAACAGGATG GATTTACAAA GAACATAGGA CTTAAAGACA TGGCTTTTCC ACATAATATG
    AGCATATTTC TTACCACTTT GTCTAACGTA CATGAAAATG GTAGGCACAA TCAAGAAAAA
    AATATTCAGG AAGAGATAGA GAAGGAAGCA CTAATTGAAG AGAAAGTAGT TTTGCCCCAG
    GTGCACGAAG CAACTGGCTC TAAGAATTTC TTGAAAGACA TATTGATACT AGGCACTAGG
    CAAAATATAA GTTTATATGA AGTACATGTA CCAGTACTTC AAAACATCAC ATCAATAAAC
    AATTCAACAA ATACAGTACA GATTCACATG GAGCATTTCT TTAAAAGAAG GAAGGACAAG
    GAAACAAATT CAGAAGGCTT GGTAAATAAA ACCAGAGAAA TGGTAAAAAA CTATCCAAGC
    CAGAAGAATA TTACTACTCA ACGTAGTAAA CGGGCTTTGG GACAATTCAG ACTGTCAACT
    CAATGGCTTA AAACCATAAA CTGTTCAACA CAGTGTATCA TTAAACAGAT AGACCACAGC
    AAGGAAATGA AAAAGTTCAT TACTAAATCT TCCTTATCAG ATTCTTCTGT GATTAAAAGC
    ACCACTCAGA CAAATAGTTC TGACTCACAC ATTGTAAAAA CATCAGCATT TCCACCAATA
    GATCTCAAAA GGAGTCCATT CCAAAACAAA TTTTCTCATG TTCAAGCATC ATCCTACATT
    TATGACTTTA AGACAAAAAG TTCAAGAATT CAAGAAAGCA ATAATTTCTT AAAAGAAACC
    AAAATAAATA ACCCTTCTTT AGCCATTCTA CCATGGAATA TGTTCATAGA TCAAGGAAAA
    TTTACCTCCC CAGGGAAAAG TAACACAAAC TCAGTCACAT ATAAGAAACG TGAGAACATT
    ATTTTCTTGA AACCAACTTT GCCTGAAGAA TCTGGCAAAA TTGAATTGCT TCCTCAAGTT
    TCCATTCAAG AGGAAGAAAT TTTACCTACA GAAACTAGCC ATGGATCTCC TGGACACTTG
    AATCTCATGA AAGAGGTCTT TCTTCAGAAA ATACAGGGGC CTACTAAATG GAATAAAGCA
    AAGAGGCATG GAGAAAGTAT AAAAGGTAAA ACAGAGAGCT CTAAAAATAC TCGCTCAAAA
    CTGCTAAATC ATCATGCTTG GGATTATCAT TATGCTGCAC AGATACCAAA AGATATGTGG
    AAATCCAAAG AGAAGTCACC AGAAATTATA TCCATTAAGC AAGAGGACAC CATTTTGTCT
    CTGAGGCCTC ATGGAAACAG TCATTCAATA GGGGCAAATG AGAAACAAAA TTGGCCTCAA
    AGAGAAACCA CTTGGGTAAA GCAAGGCCAA ACTCAAAGGA CATGCTCTCA AATCCCACCA
    GTGTTGAAAC GACATCAAAG GGAACTTAGT GCTTTTCAAT CAGAACAAGA AGCAACTGAC
    TATGATGATG CCATCACCAT TGAAACAATC GAGGATTTTG ACATTTACAG TGAGGACATA
    AAGCAAGGTC CCCGCAGCTT TCAACAGAAA ACAAGGCACT ATTTTATTGC AGCTGTGGAA
    CGACTCTGGG ACTATGGGAT GAGTACATCT CATGTTCTAC GAAATAGGTA TCAAAGTGAC
    AATGTACCTC AGTTCAAGAA AGTAGTTTTC CAGGAATTTA CTGATGGCTC CTTTAGTCAG
    CCCTTATATC GTGGAGAATT AAATGAACAC CTGGGGTTGT TGGGCCCATA TATAAGAGCA
    GAAGTTGAAG ACAACATTAT GGTAACTTTC AAAAACCAGG CCTCCCGTCC CTACTCCTTC
    TATTCTAGCC TCATTTCTTA TAAAGAAGAT CAGAGAGGAG AAGAACCTAG AAGAAACTTT
    GTCAAGCCTA ATGAAACCAA AATTTATTTT TGGAAAGTAC AACATCATAT GGCACCCACA
    GAAGATGAGT TTGACTGCAA GGCCTGGGCT TATTTCTCTG ATGTTGATCT TGAAAGAGAT
    ATGCACTCGG GATTAATTGG ACCCCTTCTG ATTTGCCACG CGAACACACT GAATCCTGCT
    CATGGGAGAC AAGTGTCAGT ACAGGAATTT GCTCTGCTTT TCACTATCTT TGATGAGACC
    AAGAGCTGGT ACTTCACTGA AAACGTGAAA AGGAACTGCA AGACACCCTG CAATTTCCAG
    ATGGAAGACC CCACTTTGAA AGAGAATTAT CGCTTCCATG CAATCAATGG TTATGTAATG
    GATACCCTAC CAGGCTTAGT AATGGCTCAA GATCAAAGGA TTCGATGGTA TCTTCTCAGC
    ATGGGCAACA ATGAGAACAT CCAATCTATT CATTTCAGTG GACATGTTTT CACTGTACGG
    AAAAAAGAGG AGTATAAAAT GGCAGTGTAC AACCTCTACC CAGGTGTTTT TGAGACTCTG
    GAAATGATAC CATCCAGAGC TGGAATATGG CGAGTAGAAT GCCTTATTGG CGAGCACTTA
    CAGGCTGGGA TGAGCACTCT TTTTCTGGTG TACAGCAAGC AGTGTCAGAT TCCTCTTGGA
    ATGGCTTCTG GAAGCATCCG TGATTTCCAG ATTACAGCTT CAGGACATTA TGGACAGTGG
    GCCCCAAACC TGGCAAGACT TCATTATTCC GGATCAATCA ATGCCTGGAG TACCAAGGAG
    CCCTTTTCTT GGATCAAGGT AGATCTGTTG GCACCAATGA TTGTTCATGG CATCAAGACT
    CAGGGTGCTC GTCAGAAATT TTCCAGCCTT TATATCTCTC AATTTATCAT CATGTATAGC
    CTGGATGGGA AGAAGTGGCT GAGTTATCAA GGAAATTCCA CTGGAACCTT AATGGTTTTC
    TTTGGCAATG TGGACTCATC TGGGATTAAG CATAATAGTT TTAATCCTCC AATTATTGCT
    CGATATATCC GTTTGCACCC CACTCATTCT AGCATCCGTA GTACTCTTCG CATGGAGTTG
    ATGGGCTGTG ATTTAAACAG TTGCAGCATA CCATTGGGAA TGGAAAGTAA AGTAATATCA
    GATACACAAA TCACTGCCTC ATCCTACTTC ACCAACATGT TTGCTACTTG GTCTCCTTCA
    CAAGCTCGAC TTCACCTCCA GGGAAGGACT AATGCCTGGC GACCTCAGGT GAATGATCCA
    AAACAATGGT TGCAAGTGGA CTTACAAAAG ACAATGAAAG TCACTGGAAT AATAACCCAG
    GGAGTGAAAT CTCTCTTTAC CAGCATGTTT GTGAAAGAGT TCCTTATTTC CAGCAGTCAA
    GATGGCCATC ACTGGACTCA AATTTTATAC AATGGCAAGG TAAAGGTTTT TCAGGGGAAT
    CAGGACTCAT CCACACCTAT GATGAATTCT CTAGACCCAC CATTACTCAC TCGCTATCTT
    CGAATTCACC CCCAGATCTG GGAGCACCAA ATTGCTCTGA GGCTTGAGAT TCTAGGATGT
    GAGGCCCAGC AGCAATACTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu01160
    Clone ID Related Accession (Same CDS sequence) NM_007977.2
    Accession Version NM_007977.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6960bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-27
    Organism Mus musculus(house mouse)
    Product coagulation factor VIII isoform 1 precursor
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK051579.1, L05573.1, BC138347.1 and AW489638.1. On May 29, 2009 this sequence version replaced NM_007977.1. Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    ATGCAAATAG CACTCTTCGC TTGCTTCTTT CTGAGCCTTT TCAATTTCTG CTCTAGTGCC 
    ATCAGAAGAT ACTACCTTGG TGCAGTGGAA TTGTCCTGGA ACTATATTCA GAGTGATCTG
    CTCAGTGTGC TGCATACAGA CTCAAGATTT CTTCCTAGAA TGTCAACATC TTTTCCATTC
    AACACCTCCA TCATGTATAA AAAGACTGTG TTTGTAGAGT ACAAGGACCA GCTTTTCAAC
    ATTGCCAAGC CCAGGCCACC CTGGATGGGT TTGCTAGGTC CTACCATTTG GACTGAGGTT
    CATGACACAG TGGTCATTAC ACTTAAAAAC ATGGCTTCTC ATCCTGTCAG TCTTCATGCT
    GTTGGTGTGT CCTACTGGAA AGCTTCTGAG GGAGATGAAT ATGAAGATCA GACAAGCCAA
    ATGGAGAAGG AAGATGATAA AGTTTTCCCT GGTGAAAGTC ATACTTATGT TTGGCAAGTC
    CTGAAAGAGA ATGGTCCAAT GGCCTCTGAC CCTCCATGTC TCACTTACTC ATATATGTCT
    CATGTGGATC TGGTGAAAGA TTTGAATTCA GGCCTCATTG GAGCTCTGCT AGTATGTAAA
    GAAGGCAGTC TCTCCAAAGA AAGAACACAG ATGTTGTACC AATTTGTACT GCTTTTTGCT
    GTATTTGATG AAGGGAAGAG CTGGCACTCA GAAACAAACG ACTCTTATAC ACAGTCTATG
    GATTCTGCAT CTGCTAGAGA CTGGCCTAAA ATGCACACAG TCAATGGCTA TGTAAACAGG
    TCTCTTCCAG GTCTGATTGG ATGCCATAGG AAATCAGTCT ACTGGCACGT GATTGGAATG
    GGCACCACTC CTGAAATACA CTCAATATTC CTCGAAGGTC ACACATTTTT TGTGAGGAAC
    CACCGTCAAG CTTCATTGGA GATATCACCA ATAACTTTCC TTACTGCTCA AACACTCTTG
    ATAGATCTTG GGCAGTTCCT ACTATTTTGT CATATCTCTT CCCATAAACA TGATGGCATG
    GAAGCTTATG TCAAAGTAGA TAGCTGCCCT GAGGAATCCC AATGGCAAAA GAAAAATAAT
    AATGAGGAAA TGGAAGATTA TGATGATGAT CTTTATTCAG AAATGGATAT GTTCACATTG
    GATTATGACA GCTCTCCTTT TATCCAAATT CGCTCGGTTG CTAAAAAGTA CCCTAAAACT
    TGGATACATT ATATTTCTGC TGAGGAGGAA GACTGGGACT ATGCACCTTC AGTTCCTACC
    TCGGATAATG GAAGTTATAA AAGCCAGTAT CTGAGCAATG GTCCTCATCG GATTGGTAGG
    AAATATAAAA AAGTCAGATT TATAGCATAC ACAGATGAAA CCTTTAAGAC TCGTGAAACT
    ATTCAGCATG AATCAGGACT CTTGGGACCT TTACTTTATG GAGAAGTTGG AGACACACTG
    TTGATTATTT TTAAGAATCA AGCAAGCCGA CCATATAACA TTTACCCTCA TGGAATCACT
    GATGTCAGTC CTCTACATGC AAGGAGATTG CCAAGAGGTA TAAAGCACGT GAAGGATTTG
    CCAATTCATC CAGGAGAGAT ATTCAAGTAC AAGTGGACAG TTACAGTAGA AGATGGACCA
    ACTAAATCAG ATCCACGGTG CCTGACCCGC TATTATTCAA GTTTCATTAA CCCTGAGAGA
    GATCTAGCTT CAGGACTGAT TGGCCCTCTT CTCATCTGCT ACAAAGAATC TGTAGATCAA
    AGGGGAAACC AGATGATGTC AGACAAAAGA AATGTCATCC TGTTTTCTAT ATTTGATGAG
    AACCAAAGCT GGTACATCAC AGAGAACATG CAACGCTTCC TCCCCAATGC AGCTAAAACA
    CAGCCCCAGG ACCCTGGGTT CCAGGCCTCC AACATCATGC ACAGCATCAA TGGCTATGTT
    TTTGATAGCT TGGAGTTGAC AGTTTGTTTG CATGAGGTGG CATACTGGCA CATTCTCAGT
    GTTGGAGCAC AGACAGACTT CTTATCTATC TTCTTCTCTG GATATACTTT CAAACACAAA
    ATGGTCTATG AAGATACACT TACCCTGTTC CCATTCTCAG GAGAAACTGT CTTTATGTCG
    ATGGAAAACC CAGGTCTATG GGTCTTGGGG TGTCATAATT CAGACTTTCG GAAGAGAGGT
    ATGACAGCAT TGCTGAAAGT TTCTAGTTGT GACAAGAGCA CTAGTGATTA TTATGAAGAA
    ATATATGAAG ATATTCCAAC ACAGTTGGTG AATGAGAACA ATGTCATTGA TCCCAGAAGC
    TTCTTCCAGA ATACAAATCA TCCTAATACT AGGAAAAAGA AATTCAAAGA TTCCACAATT
    CCAAAAAATG ATATGGAGAA GATTGAGCCT CAGTTTGAAG AGATAGCAGA GATGCTTAAA
    GTACAGAGTG TCTCAGTTAG TGACATGTTG ATGCTCTTGG GACAGAGTCA TCCTACTCCA
    CATGGCTTAT TTTTATCAGA TGGCCAAGAA GCCATCTATG AGGCTATTCA TGATGATCAT
    TCACCAAATG CAATAGACAG CAATGAAGGC CCATCTAAAG TGACCCAACT CAGGCCAGAA
    TCCCATCACA GTGAGAAAAT AGTATTTACT CCTCAGCCCG GCCTCCAGTT AAGATCCAAT
    AAAAGTTTGG AGACAACTAT AGAAGTAAAG TGGAAGAAAC TTGGTTTGCA AGTTTCTAGT
    TTGCCAAGTA ATCTAATGAC TACAACAATT CTGTCAGACA ATTTGAAAGC AACTTTTGAA
    AAGACAGATT CTTCAGGATT TCCAGATATG CCAGTTCACT CTAGTAGTAA ATTAAGTACT
    ACTGCATTTG GTAAGAAAGC ATATTCCCTT GTTGGGTCTC ATGTACCTTT AAACGTGAGT
    GAAGAAAATA GTGATTCCAA CATATTGGAT TCAACTTTAA TGTATAGTCA AGAAAGTTTA
    CCAAGAGATA ATATATTATC AATGGAGAAT GATAGATTAC TCAGAGAGAA GAGGTTTCAT
    GGAATTGCTT TATTGACCAA AGATAATACT TTATTCAAAG ACAATGTCTC CTTAATGAAA
    ACAAACAAAA CATATAATCA TTCAACAACT AATGAAAAAC TACACACTGA GAGCCCAACA
    TCAATTGAGA ATAGTACAAC AGACTTGCAA GATGCCATAT TAAAGGTCAA TAGTGAGATT
    CAAGAAGTAA CAGCTTTGAT TCATGATGGA ACACTTTTAG GCAAAAATTC TACATATTTG
    AGACTAAACC ATATGCTAAA TAGAACTACC TCAACAAAAA ATAAAGACAT ATTTCATAGA
    AAAGATGAAG ATCCTATTCC ACAAGATGAA GAGAATACAA TCATGCCATT TTCCAAGATG
    TTGTTCTTGT CAGAATCTTC AAATTGGTTT AAAAAGACCA ATGGAAATAA TTCCTTGAAC
    TCTGAGCAAG AACATAGTCC AAAGCAATTA GTATATTTAA TGTTTAAAAA ATATGTAAAA
    AATCAAAGTT TCTTGTCAGA GAAAAATAAA GTCACAGTAG AACAGGATGG ATTTACAAAG
    AACATAGGAC TTAAAGACAT GGCTTTTCCA CATAATATGA GCATATTTCT TACCACTTTG
    TCTAACGTAC ATGAAAATGG TAGGCACAAT CAAGAAAAAA ATATTCAGGA AGAGATAGAG
    AAGGAAGCAC TAATTGAAGA GAAAGTAGTT TTGCCCCAGG TGCACGAAGC AACTGGCTCT
    AAGAATTTCT TGAAAGACAT ATTGATACTA GGCACTAGGC AAAATATAAG TTTATATGAA
    GTACATGTAC CAGTACTTCA AAACATCACA TCAATAAACA ATTCAACAAA TACAGTACAG
    ATTCACATGG AGCATTTCTT TAAAAGAAGG AAGGACAAGG AAACAAATTC AGAAGGCTTG
    GTAAATAAAA CCAGAGAAAT GGTAAAAAAC TATCCAAGCC AGAAGAATAT TACTACTCAA
    CGTAGTAAAC GGGCTTTGGG ACAATTCAGA CTGTCAACTC AATGGCTTAA AACCATAAAC
    TGTTCAACAC AGTGTATCAT TAAACAGATA GACCACAGCA AGGAAATGAA AAAGTTCATT
    ACTAAATCTT CCTTATCAGA TTCTTCTGTG ATTAAAAGCA CCACTCAGAC AAATAGTTCT
    GACTCACACA TTGTAAAAAC ATCAGCATTT CCACCAATAG ATCTCAAAAG GAGTCCATTC
    CAAAACAAAT TTTCTCATGT TCAAGCATCA TCCTACATTT ATGACTTTAA GACAAAAAGT
    TCAAGAATTC AAGAAAGCAA TAATTTCTTA AAAGAAACCA AAATAAATAA CCCTTCTTTA
    GCCATTCTAC CATGGAATAT GTTCATAGAT CAAGGAAAAT TTACCTCCCC AGGGAAAAGT
    AACACAAACT CAGTCACATA TAAGAAACGT GAGAACATTA TTTTCTTGAA ACCAACTTTG
    CCTGAAGAAT CTGGCAAAAT TGAATTGCTT CCTCAAGTTT CCATTCAAGA GGAAGAAATT
    TTACCTACAG AAACTAGCCA TGGATCTCCT GGACACTTGA ATCTCATGAA AGAGGTCTTT
    CTTCAGAAAA TACAGGGGCC TACTAAATGG AATAAAGCAA AGAGGCATGG AGAAAGTATA
    AAAGGTAAAA CAGAGAGCTC TAAAAATACT CGCTCAAAAC TGCTAAATCA TCATGCTTGG
    GATTATCATT ATGCTGCACA GATACCAAAA GATATGTGGA AATCCAAAGA GAAGTCACCA
    GAAATTATAT CCATTAAGCA AGAGGACACC ATTTTGTCTC TGAGGCCTCA TGGAAACAGT
    CATTCAATAG GGGCAAATGA GAAACAAAAT TGGCCTCAAA GAGAAACCAC TTGGGTAAAG
    CAAGGCCAAA CTCAAAGGAC ATGCTCTCAA ATCCCACCAG TGTTGAAACG ACATCAAAGG
    GAACTTAGTG CTTTTCAATC AGAACAAGAA GCAACTGACT ATGATGATGC CATCACCATT
    GAAACAATCG AGGATTTTGA CATTTACAGT GAGGACATAA AGCAAGGTCC CCGCAGCTTT
    CAACAGAAAA CAAGGCACTA TTTTATTGCA GCTGTGGAAC GACTCTGGGA CTATGGGATG
    AGTACATCTC ATGTTCTACG AAATAGGTAT CAAAGTGACA ATGTACCTCA GTTCAAGAAA
    GTAGTTTTCC AGGAATTTAC TGATGGCTCC TTTAGTCAGC CCTTATATCG TGGAGAATTA
    AATGAACACC TGGGGTTGTT GGGCCCATAT ATAAGAGCAG AAGTTGAAGA CAACATTATG
    GTAACTTTCA AAAACCAGGC CTCCCGTCCC TACTCCTTCT ATTCTAGCCT CATTTCTTAT
    AAAGAAGATC AGAGAGGAGA AGAACCTAGA AGAAACTTTG TCAAGCCTAA TGAAACCAAA
    ATTTATTTTT GGAAAGTACA ACATCATATG GCACCCACAG AAGATGAGTT TGACTGCAAG
    GCCTGGGCTT ATTTCTCTGA TGTTGATCTT GAAAGAGATA TGCACTCGGG ATTAATTGGA
    CCCCTTCTGA TTTGCCACGC GAACACACTG AATCCTGCTC ATGGGAGACA AGTGTCAGTA
    CAGGAATTTG CTCTGCTTTT CACTATCTTT GATGAGACCA AGAGCTGGTA CTTCACTGAA
    AACGTGAAAA GGAACTGCAA GACACCCTGC AATTTCCAGA TGGAAGACCC CACTTTGAAA
    GAGAATTATC GCTTCCATGC AATCAATGGT TATGTAATGG ATACCCTACC AGGCTTAGTA
    ATGGCTCAAG ATCAAAGGAT TCGATGGTAT CTTCTCAGCA TGGGCAACAA TGAGAACATC
    CAATCTATTC ATTTCAGTGG ACATGTTTTC ACTGTACGGA AAAAAGAGGA GTATAAAATG
    GCAGTGTACA ACCTCTACCC AGGTGTTTTT GAGACTCTGG AAATGATACC ATCCAGAGCT
    GGAATATGGC GAGTAGAATG CCTTATTGGC GAGCACTTAC AGGCTGGGAT GAGCACTCTT
    TTTCTGGTGT ACAGCAAGCA GTGTCAGATT CCTCTTGGAA TGGCTTCTGG AAGCATCCGT
    GATTTCCAGA TTACAGCTTC AGGACATTAT GGACAGTGGG CCCCAAACCT GGCAAGACTT
    CATTATTCCG GATCAATCAA TGCCTGGAGT ACCAAGGAGC CCTTTTCTTG GATCAAGGTA
    GATCTGTTGG CACCAATGAT TGTTCATGGC ATCAAGACTC AGGGTGCTCG TCAGAAATTT
    TCCAGCCTTT ATATCTCTCA ATTTATCATC ATGTATAGCC TGGATGGGAA GAAGTGGCTG
    AGTTATCAAG GAAATTCCAC TGGAACCTTA ATGGTTTTCT TTGGCAATGT GGACTCATCT
    GGGATTAAGC ATAATAGTTT TAATCCTCCA ATTATTGCTC GATATATCCG TTTGCACCCC
    ACTCATTCTA GCATCCGTAG TACTCTTCGC ATGGAGTTGA TGGGCTGTGA TTTAAACAGT
    TGCAGCATAC CATTGGGAAT GGAAAGTAAA GTAATATCAG ATACACAAAT CACTGCCTCA
    TCCTACTTCA CCAACATGTT TGCTACTTGG TCTCCTTCAC AAGCTCGACT TCACCTCCAG
    GGAAGGACTA ATGCCTGGCG ACCTCAGGTG AATGATCCAA AACAATGGTT GCAAGTGGAC
    TTACAAAAGA CAATGAAAGT CACTGGAATA ATAACCCAGG GAGTGAAATC TCTCTTTACC
    AGCATGTTTG TGAAAGAGTT CCTTATTTCC AGCAGTCAAG ATGGCCATCA CTGGACTCAA
    ATTTTATACA ATGGCAAGGT AAAGGTTTTT CAGGGGAATC AGGACTCATC CACACCTATG
    ATGAATTCTC TAGACCCACC ATTACTCACT CGCTATCTTC GAATTCACCC CCAGATCTGG
    GAGCACCAAA TTGCTCTGAG GCTTGAGATT CTAGGATGTG AGGCCCAGCA GCAATACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_032003.2
    CDS410..7369
    Translation

    Target ORF information:

    RefSeq Version NM_007977.2
    Organism Mus musculus(house mouse)
    Definition Mus musculus coagulation factor VIII (F8), transcript variant 1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_007977.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    ATGCAAATAG CACTCTTCGC TTGCTTCTTT CTGAGCCTTT TCAATTTCTG CTCTAGTGCC 
    ATCAGAAGAT ACTACCTTGG TGCAGTGGAA TTGTCCTGGA ACTATATTCA GAGTGATCTG
    CTCAGTGTGC TGCATACAGA CTCAAGATTT CTTCCTAGAA TGTCAACATC TTTTCCATTC
    AACACCTCCA TCATGTATAA AAAGACTGTG TTTGTAGAGT ACAAGGACCA GCTTTTCAAC
    ATTGCCAAGC CCAGGCCACC CTGGATGGGT TTGCTAGGTC CTACCATTTG GACTGAGGTT
    CATGACACAG TGGTCATTAC ACTTAAAAAC ATGGCTTCTC ATCCTGTCAG TCTTCATGCT
    GTTGGTGTGT CCTACTGGAA AGCTTCTGAG GGAGATGAAT ATGAAGATCA GACAAGCCAA
    ATGGAGAAGG AAGATGATAA AGTTTTCCCT GGTGAAAGTC ATACTTATGT TTGGCAAGTC
    CTGAAAGAGA ATGGTCCAAT GGCCTCTGAC CCTCCATGTC TCACTTACTC ATATATGTCT
    CATGTGGATC TGGTGAAAGA TTTGAATTCA GGCCTCATTG GAGCTCTGCT AGTATGTAAA
    GAAGGCAGTC TCTCCAAAGA AAGAACACAG ATGTTGTACC AATTTGTACT GCTTTTTGCT
    GTATTTGATG AAGGGAAGAG CTGGCACTCA GAAACAAACG ACTCTTATAC ACAGTCTATG
    GATTCTGCAT CTGCTAGAGA CTGGCCTAAA ATGCACACAG TCAATGGCTA TGTAAACAGG
    TCTCTTCCAG GTCTGATTGG ATGCCATAGG AAATCAGTCT ACTGGCACGT GATTGGAATG
    GGCACCACTC CTGAAATACA CTCAATATTC CTCGAAGGTC ACACATTTTT TGTGAGGAAC
    CACCGTCAAG CTTCATTGGA GATATCACCA ATAACTTTCC TTACTGCTCA AACACTCTTG
    ATAGATCTTG GGCAGTTCCT ACTATTTTGT CATATCTCTT CCCATAAACA TGATGGCATG
    GAAGCTTATG TCAAAGTAGA TAGCTGCCCT GAGGAATCCC AATGGCAAAA GAAAAATAAT
    AATGAGGAAA TGGAAGATTA TGATGATGAT CTTTATTCAG AAATGGATAT GTTCACATTG
    GATTATGACA GCTCTCCTTT TATCCAAATT CGCTCGGTTG CTAAAAAGTA CCCTAAAACT
    TGGATACATT ATATTTCTGC TGAGGAGGAA GACTGGGACT ATGCACCTTC AGTTCCTACC
    TCGGATAATG GAAGTTATAA AAGCCAGTAT CTGAGCAATG GTCCTCATCG GATTGGTAGG
    AAATATAAAA AAGTCAGATT TATAGCATAC ACAGATGAAA CCTTTAAGAC TCGTGAAACT
    ATTCAGCATG AATCAGGACT CTTGGGACCT TTACTTTATG GAGAAGTTGG AGACACACTG
    TTGATTATTT TTAAGAATCA AGCAAGCCGA CCATATAACA TTTACCCTCA TGGAATCACT
    GATGTCAGTC CTCTACATGC AAGGAGATTG CCAAGAGGTA TAAAGCACGT GAAGGATTTG
    CCAATTCATC CAGGAGAGAT ATTCAAGTAC AAGTGGACAG TTACAGTAGA AGATGGACCA
    ACTAAATCAG ATCCACGGTG CCTGACCCGC TATTATTCAA GTTTCATTAA CCCTGAGAGA
    GATCTAGCTT CAGGACTGAT TGGCCCTCTT CTCATCTGCT ACAAAGAATC TGTAGATCAA
    AGGGGAAACC AGATGATGTC AGACAAAAGA AATGTCATCC TGTTTTCTAT ATTTGATGAG
    AACCAAAGCT GGTACATCAC AGAGAACATG CAACGCTTCC TCCCCAATGC AGCTAAAACA
    CAGCCCCAGG ACCCTGGGTT CCAGGCCTCC AACATCATGC ACAGCATCAA TGGCTATGTT
    TTTGATAGCT TGGAGTTGAC AGTTTGTTTG CATGAGGTGG CATACTGGCA CATTCTCAGT
    GTTGGAGCAC AGACAGACTT CTTATCTATC TTCTTCTCTG GATATACTTT CAAACACAAA
    ATGGTCTATG AAGATACACT TACCCTGTTC CCATTCTCAG GAGAAACTGT CTTTATGTCG
    ATGGAAAACC CAGGTCTATG GGTCTTGGGG TGTCATAATT CAGACTTTCG GAAGAGAGGT
    ATGACAGCAT TGCTGAAAGT TTCTAGTTGT GACAAGAGCA CTAGTGATTA TTATGAAGAA
    ATATATGAAG ATATTCCAAC ACAGTTGGTG AATGAGAACA ATGTCATTGA TCCCAGAAGC
    TTCTTCCAGA ATACAAATCA TCCTAATACT AGGAAAAAGA AATTCAAAGA TTCCACAATT
    CCAAAAAATG ATATGGAGAA GATTGAGCCT CAGTTTGAAG AGATAGCAGA GATGCTTAAA
    GTACAGAGTG TCTCAGTTAG TGACATGTTG ATGCTCTTGG GACAGAGTCA TCCTACTCCA
    CATGGCTTAT TTTTATCAGA TGGCCAAGAA GCCATCTATG AGGCTATTCA TGATGATCAT
    TCACCAAATG CAATAGACAG CAATGAAGGC CCATCTAAAG TGACCCAACT CAGGCCAGAA
    TCCCATCACA GTGAGAAAAT AGTATTTACT CCTCAGCCCG GCCTCCAGTT AAGATCCAAT
    AAAAGTTTGG AGACAACTAT AGAAGTAAAG TGGAAGAAAC TTGGTTTGCA AGTTTCTAGT
    TTGCCAAGTA ATCTAATGAC TACAACAATT CTGTCAGACA ATTTGAAAGC AACTTTTGAA
    AAGACAGATT CTTCAGGATT TCCAGATATG CCAGTTCACT CTAGTAGTAA ATTAAGTACT
    ACTGCATTTG GTAAGAAAGC ATATTCCCTT GTTGGGTCTC ATGTACCTTT AAACGTGAGT
    GAAGAAAATA GTGATTCCAA CATATTGGAT TCAACTTTAA TGTATAGTCA AGAAAGTTTA
    CCAAGAGATA ATATATTATC AATGGAGAAT GATAGATTAC TCAGAGAGAA GAGGTTTCAT
    GGAATTGCTT TATTGACCAA AGATAATACT TTATTCAAAG ACAATGTCTC CTTAATGAAA
    ACAAACAAAA CATATAATCA TTCAACAACT AATGAAAAAC TACACACTGA GAGCCCAACA
    TCAATTGAGA ATAGTACAAC AGACTTGCAA GATGCCATAT TAAAGGTCAA TAGTGAGATT
    CAAGAAGTAA CAGCTTTGAT TCATGATGGA ACACTTTTAG GCAAAAATTC TACATATTTG
    AGACTAAACC ATATGCTAAA TAGAACTACC TCAACAAAAA ATAAAGACAT ATTTCATAGA
    AAAGATGAAG ATCCTATTCC ACAAGATGAA GAGAATACAA TCATGCCATT TTCCAAGATG
    TTGTTCTTGT CAGAATCTTC AAATTGGTTT AAAAAGACCA ATGGAAATAA TTCCTTGAAC
    TCTGAGCAAG AACATAGTCC AAAGCAATTA GTATATTTAA TGTTTAAAAA ATATGTAAAA
    AATCAAAGTT TCTTGTCAGA GAAAAATAAA GTCACAGTAG AACAGGATGG ATTTACAAAG
    AACATAGGAC TTAAAGACAT GGCTTTTCCA CATAATATGA GCATATTTCT TACCACTTTG
    TCTAACGTAC ATGAAAATGG TAGGCACAAT CAAGAAAAAA ATATTCAGGA AGAGATAGAG
    AAGGAAGCAC TAATTGAAGA GAAAGTAGTT TTGCCCCAGG TGCACGAAGC AACTGGCTCT
    AAGAATTTCT TGAAAGACAT ATTGATACTA GGCACTAGGC AAAATATAAG TTTATATGAA
    GTACATGTAC CAGTACTTCA AAACATCACA TCAATAAACA ATTCAACAAA TACAGTACAG
    ATTCACATGG AGCATTTCTT TAAAAGAAGG AAGGACAAGG AAACAAATTC AGAAGGCTTG
    GTAAATAAAA CCAGAGAAAT GGTAAAAAAC TATCCAAGCC AGAAGAATAT TACTACTCAA
    CGTAGTAAAC GGGCTTTGGG ACAATTCAGA CTGTCAACTC AATGGCTTAA AACCATAAAC
    TGTTCAACAC AGTGTATCAT TAAACAGATA GACCACAGCA AGGAAATGAA AAAGTTCATT
    ACTAAATCTT CCTTATCAGA TTCTTCTGTG ATTAAAAGCA CCACTCAGAC AAATAGTTCT
    GACTCACACA TTGTAAAAAC ATCAGCATTT CCACCAATAG ATCTCAAAAG GAGTCCATTC
    CAAAACAAAT TTTCTCATGT TCAAGCATCA TCCTACATTT ATGACTTTAA GACAAAAAGT
    TCAAGAATTC AAGAAAGCAA TAATTTCTTA AAAGAAACCA AAATAAATAA CCCTTCTTTA
    GCCATTCTAC CATGGAATAT GTTCATAGAT CAAGGAAAAT TTACCTCCCC AGGGAAAAGT
    AACACAAACT CAGTCACATA TAAGAAACGT GAGAACATTA TTTTCTTGAA ACCAACTTTG
    CCTGAAGAAT CTGGCAAAAT TGAATTGCTT CCTCAAGTTT CCATTCAAGA GGAAGAAATT
    TTACCTACAG AAACTAGCCA TGGATCTCCT GGACACTTGA ATCTCATGAA AGAGGTCTTT
    CTTCAGAAAA TACAGGGGCC TACTAAATGG AATAAAGCAA AGAGGCATGG AGAAAGTATA
    AAAGGTAAAA CAGAGAGCTC TAAAAATACT CGCTCAAAAC TGCTAAATCA TCATGCTTGG
    GATTATCATT ATGCTGCACA GATACCAAAA GATATGTGGA AATCCAAAGA GAAGTCACCA
    GAAATTATAT CCATTAAGCA AGAGGACACC ATTTTGTCTC TGAGGCCTCA TGGAAACAGT
    CATTCAATAG GGGCAAATGA GAAACAAAAT TGGCCTCAAA GAGAAACCAC TTGGGTAAAG
    CAAGGCCAAA CTCAAAGGAC ATGCTCTCAA ATCCCACCAG TGTTGAAACG ACATCAAAGG
    GAACTTAGTG CTTTTCAATC AGAACAAGAA GCAACTGACT ATGATGATGC CATCACCATT
    GAAACAATCG AGGATTTTGA CATTTACAGT GAGGACATAA AGCAAGGTCC CCGCAGCTTT
    CAACAGAAAA CAAGGCACTA TTTTATTGCA GCTGTGGAAC GACTCTGGGA CTATGGGATG
    AGTACATCTC ATGTTCTACG AAATAGGTAT CAAAGTGACA ATGTACCTCA GTTCAAGAAA
    GTAGTTTTCC AGGAATTTAC TGATGGCTCC TTTAGTCAGC CCTTATATCG TGGAGAATTA
    AATGAACACC TGGGGTTGTT GGGCCCATAT ATAAGAGCAG AAGTTGAAGA CAACATTATG
    GTAACTTTCA AAAACCAGGC CTCCCGTCCC TACTCCTTCT ATTCTAGCCT CATTTCTTAT
    AAAGAAGATC AGAGAGGAGA AGAACCTAGA AGAAACTTTG TCAAGCCTAA TGAAACCAAA
    ATTTATTTTT GGAAAGTACA ACATCATATG GCACCCACAG AAGATGAGTT TGACTGCAAG
    GCCTGGGCTT ATTTCTCTGA TGTTGATCTT GAAAGAGATA TGCACTCGGG ATTAATTGGA
    CCCCTTCTGA TTTGCCACGC GAACACACTG AATCCTGCTC ATGGGAGACA AGTGTCAGTA
    CAGGAATTTG CTCTGCTTTT CACTATCTTT GATGAGACCA AGAGCTGGTA CTTCACTGAA
    AACGTGAAAA GGAACTGCAA GACACCCTGC AATTTCCAGA TGGAAGACCC CACTTTGAAA
    GAGAATTATC GCTTCCATGC AATCAATGGT TATGTAATGG ATACCCTACC AGGCTTAGTA
    ATGGCTCAAG ATCAAAGGAT TCGATGGTAT CTTCTCAGCA TGGGCAACAA TGAGAACATC
    CAATCTATTC ATTTCAGTGG ACATGTTTTC ACTGTACGGA AAAAAGAGGA GTATAAAATG
    GCAGTGTACA ACCTCTACCC AGGTGTTTTT GAGACTCTGG AAATGATACC ATCCAGAGCT
    GGAATATGGC GAGTAGAATG CCTTATTGGC GAGCACTTAC AGGCTGGGAT GAGCACTCTT
    TTTCTGGTGT ACAGCAAGCA GTGTCAGATT CCTCTTGGAA TGGCTTCTGG AAGCATCCGT
    GATTTCCAGA TTACAGCTTC AGGACATTAT GGACAGTGGG CCCCAAACCT GGCAAGACTT
    CATTATTCCG GATCAATCAA TGCCTGGAGT ACCAAGGAGC CCTTTTCTTG GATCAAGGTA
    GATCTGTTGG CACCAATGAT TGTTCATGGC ATCAAGACTC AGGGTGCTCG TCAGAAATTT
    TCCAGCCTTT ATATCTCTCA ATTTATCATC ATGTATAGCC TGGATGGGAA GAAGTGGCTG
    AGTTATCAAG GAAATTCCAC TGGAACCTTA ATGGTTTTCT TTGGCAATGT GGACTCATCT
    GGGATTAAGC ATAATAGTTT TAATCCTCCA ATTATTGCTC GATATATCCG TTTGCACCCC
    ACTCATTCTA GCATCCGTAG TACTCTTCGC ATGGAGTTGA TGGGCTGTGA TTTAAACAGT
    TGCAGCATAC CATTGGGAAT GGAAAGTAAA GTAATATCAG ATACACAAAT CACTGCCTCA
    TCCTACTTCA CCAACATGTT TGCTACTTGG TCTCCTTCAC AAGCTCGACT TCACCTCCAG
    GGAAGGACTA ATGCCTGGCG ACCTCAGGTG AATGATCCAA AACAATGGTT GCAAGTGGAC
    TTACAAAAGA CAATGAAAGT CACTGGAATA ATAACCCAGG GAGTGAAATC TCTCTTTACC
    AGCATGTTTG TGAAAGAGTT CCTTATTTCC AGCAGTCAAG ATGGCCATCA CTGGACTCAA
    ATTTTATACA ATGGCAAGGT AAAGGTTTTT CAGGGGAATC AGGACTCATC CACACCTATG
    ATGAATTCTC TAGACCCACC ATTACTCACT CGCTATCTTC GAATTCACCC CCAGATCTGG
    GAGCACCAAA TTGCTCTGAG GCTTGAGATT CTAGGATGTG AGGCCCAGCA GCAATACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu08135
    Clone ID Related Accession (Same CDS sequence) NM_001161373.1
    Accession Version NM_001161373.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6750bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-10-18
    Organism Mus musculus(house mouse)
    Product coagulation factor VIII isoform 2 precursor
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK051579.1, BC138347.1 and AW489638.1. Transcript Variant: This variant (2) lacks an alternate in-frame exon compared to variant 1. The resulting isoform (2) has the same N- and C-termini but is shorter compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC138347.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    ATGCAAATAG CACTCTTCGC TTGCTTCTTT CTGAGCCTTT TCAATTTCTG CTCTAGTGCC 
    ATCAGAAGAT ACTACCTTGG TGCAGTGGAA TTGTCCTGGA ACTATATTCA GAGTGATCTG
    CTCAGTGTGC TGCATACAGA CTCAAGATTT CTTCCTAGAA TGTCAACATC TTTTCCATTC
    AACACCTCCA TCATGTATAA AAAGACTGTG TTTGTAGAGT ACAAGGACCA GCTTTTCAAC
    ATTGCCAAGC CCAGGCCACC CTGGATGGGT TTGCTAGGTC CTACCATTTG GACTGAGGTT
    CATGACACAG TGGTCATTAC ACTTAAAAAC ATGGCTTCTC ATCCTGTCAG TCTTCATGCT
    GTTGGTGTGT CCTACTGGAA AGCTTCTGAG GGAGATGAAT ATGAAGATCA GACAAGCCAA
    ATGGAGAAGG AAGATGATAA AGTTTTCCCT GGTGAAAGTC ATACTTATGT TTGGCAAGTC
    CTGAAAGAGA ATGGTCCAAT GGCCTCTGAC CCTCCATGTC TCACTTACTC ATATATGTCT
    CATGTGGATC TGGTGAAAGA TTTGAATTCA GGCCTCATTG GAGCTCTGCT AGTATGTAAA
    GAAGGCAGTC TCTCCAAAGA AAGAACACAG ATGTTGTACC AATTTGTACT GCTTTTTGCT
    GTATTTGATG AAGGGAAGAG CTGGCACTCA GAAACAAACG ACTCTTATAC ACAGTCTATG
    GATTCTGCAT CTGCTAGAGA CTGGCCTAAA ATGCACACAG TCAATGGCTA TGTAAACAGG
    TCTCTTCCAG GTCTGATTGG ATGCCATAGG AAATCAGTCT ACTGGCACGT GATTGGAATG
    GGCACCACTC CTGAAATACA CTCAATATTC CTCGAAGGTC ACACATTTTT TGTGAGGAAC
    CACCGTCAAG CTTCATTGGA GATATCACCA ATAACTTTCC TTACTGCTCA AACACTCTTG
    ATAGATCTTG GGCAGTTCCT ACTATTTTGT CATATCTCTT CCCATAAACA TGATGGCATG
    GAAGCTTATG TCAAAGTAGA TAGCTGCCCT GAGGAATCCC AATGGCAAAA GAAAAATAAT
    AATGAGGAAA TGGAAGATTA TGATGATGAT CTTTATTCAG AAATGGATAT GTTCACATTG
    GATTATGACA GCTCTCCTTT TATCCAAATT CGCTCGGTTG CTAAAAAGTA CCCTAAAACT
    TGGATACATT ATATTTCTGC TGAGGAGGAA GACTGGGACT ATGCACCTTC AGTTCCTACC
    TCGGATAATG GAAGTTATAA AAGCCAGTAT CTGAGCAATG GTCCTCATCG GATTGGTAGG
    AAATATAAAA AAGTCAGATT TATAGCATAC ACAGATGAAA CCTTTAAGAC TCGTGAAACT
    ATTCAGCATG AATCAGGACT CTTGGGACCT TTACTTTATG GAGAAGTTGG AGACACACTG
    TTGATTATTT TTAAGAATCA AGCAAGCCGA CCATATAACA TTTACCCTCA TGGAATCACT
    GATGTCAGTC CTCTACATGC AAGGAGATTG CCAAGAGGTA TAAAGCACGT GAAGGATTTG
    CCAATTCATC CAGGAGAGAT ATTCAAGTAC AAGTGGACAG TTACAGTAGA AGATGGACCA
    ACTAAATCAG ATCCACGGTG CCTGACCCGC TATTATTCAA GTTTCATTAA CCCTGAGAGA
    GATCTAGCTT CAGGACTGAT TGGCCCTCTT CTCATCTGCT ACAAAGAATC TGTAGATCAA
    AGGGGAAACC AGATGATGTC AGACAAAAGA AATGTCATCC TGTTTTCTAT ATTTGATGAG
    AACCAAAGCT GGTACATCAC AGAGAACATG CAACGCTTCC TCCCCAATGC AGCTAAAACA
    CAGCCCCAGG ACCCTGGGTT CCAGGCCTCC AACATCATGC ACAGTCTATG GGTCTTGGGG
    TGTCATAATT CAGACTTTCG GAAGAGAGGT ATGACAGCAT TGCTGAAAGT TTCTAGTTGT
    GACAAGAGCA CTAGTGATTA TTATGAAGAA ATATATGAAG ATATTCCAAC ACAGTTGGTG
    AATGAGAACA ATGTCATTGA TCCCAGAAGC TTCTTCCAGA ATACAAATCA TCCTAATACT
    AGGAAAAAGA AATTCAAAGA TTCCACAATT CCAAAAAATG ATATGGAGAA GATTGAGCCT
    CAGTTTGAAG AGATAGCAGA GATGCTTAAA GTACAGAGTG TCTCAGTTAG TGACATGTTG
    ATGCTCTTGG GACAGAGTCA TCCTACTCCA CATGGCTTAT TTTTATCAGA TGGCCAAGAA
    GCCATCTATG AGGCTATTCA TGATGATCAT TCACCAAATG CAATAGACAG CAATGAAGGC
    CCATCTAAAG TGACCCAACT CAGGCCAGAA TCCCATCACA GTGAGAAAAT AGTATTTACT
    CCTCAGCCCG GCCTCCAGTT AAGATCCAAT AAAAGTTTGG AGACAACTAT AGAAGTAAAG
    TGGAAGAAAC TTGGTTTGCA AGTTTCTAGT TTGCCAAGTA ATCTAATGAC TACAACAATT
    CTGTCAGACA ATTTGAAAGC AACTTTTGAA AAGACAGATT CTTCAGGATT TCCAGATATG
    CCAGTTCACT CTAGTAGTAA ATTAAGTACT ACTGCATTTG GTAAGAAAGC ATATTCCCTT
    GTTGGGTCTC ATGTACCTTT AAACGTGAGT GAAGAAAATA GTGATTCCAA CATATTGGAT
    TCAACTTTAA TGTATAGTCA AGAAAGTTTA CCAAGAGATA ATATATTATC AATGGAGAAT
    GATAGATTAC TCAGAGAGAA GAGGTTTCAT GGAATTGCTT TATTGACCAA AGATAATACT
    TTATTCAAAG ACAATGTCTC CTTAATGAAA ACAAACAAAA CATATAATCA TTCAACAACT
    AATGAAAAAC TACACACTGA GAGCCCAACA TCAATTGAGA ATAGTACAAC AGACTTGCAA
    GATGCCATAT TAAAGGTCAA TAGTGAGATT CAAGAAGTAA CAGCTTTGAT TCATGATGGA
    ACACTTTTAG GCAAAAATTC TACATATTTG AGACTAAACC ATATGCTAAA TAGAACTACC
    TCAACAAAAA ATAAAGACAT ATTTCATAGA AAAGATGAAG ATCCTATTCC ACAAGATGAA
    GAGAATACAA TCATGCCATT TTCCAAGATG TTGTTCTTGT CAGAATCTTC AAATTGGTTT
    AAAAAGACCA ATGGAAATAA TTCCTTGAAC TCTGAGCAAG AACATAGTCC AAAGCAATTA
    GTATATTTAA TGTTTAAAAA ATATGTAAAA AATCAAAGTT TCTTGTCAGA GAAAAATAAA
    GTCACAGTAG AACAGGATGG ATTTACAAAG AACATAGGAC TTAAAGACAT GGCTTTTCCA
    CATAATATGA GCATATTTCT TACCACTTTG TCTAACGTAC ATGAAAATGG TAGGCACAAT
    CAAGAAAAAA ATATTCAGGA AGAGATAGAG AAGGAAGCAC TAATTGAAGA GAAAGTAGTT
    TTGCCCCAGG TGCACGAAGC AACTGGCTCT AAGAATTTCT TGAAAGACAT ATTGATACTA
    GGCACTAGGC AAAATATAAG TTTATATGAA GTACATGTAC CAGTACTTCA AAACATCACA
    TCAATAAACA ATTCAACAAA TACAGTACAG ATTCACATGG AGCATTTCTT TAAAAGAAGG
    AAGGACAAGG AAACAAATTC AGAAGGCTTG GTAAATAAAA CCAGAGAAAT GGTAAAAAAC
    TATCCAAGCC AGAAGAATAT TACTACTCAA CGTAGTAAAC GGGCTTTGGG ACAATTCAGA
    CTGTCAACTC AATGGCTTAA AACCATAAAC TGTTCAACAC AGTGTATCAT TAAACAGATA
    GACCACAGCA AGGAAATGAA AAAGTTCATT ACTAAATCTT CCTTATCAGA TTCTTCTGTG
    ATTAAAAGCA CCACTCAGAC AAATAGTTCT GACTCACACA TTGTAAAAAC ATCAGCATTT
    CCACCAATAG ATCTCAAAAG GAGTCCATTC CAAAACAAAT TTTCTCATGT TCAAGCATCA
    TCCTACATTT ATGACTTTAA GACAAAAAGT TCAAGAATTC AAGAAAGCAA TAATTTCTTA
    AAAGAAACCA AAATAAATAA CCCTTCTTTA GCCATTCTAC CATGGAATAT GTTCATAGAT
    CAAGGAAAAT TTACCTCCCC AGGGAAAAGT AACACAAACT CAGTCACATA TAAGAAACGT
    GAGAACATTA TTTTCTTGAA ACCAACTTTG CCTGAAGAAT CTGGCAAAAT TGAATTGCTT
    CCTCAAGTTT CCATTCAAGA GGAAGAAATT TTACCTACAG AAACTAGCCA TGGATCTCCT
    GGACACTTGA ATCTCATGAA AGAGGTCTTT CTTCAGAAAA TACAGGGGCC TACTAAATGG
    AATAAAGCAA AGAGGCATGG AGAAAGTATA AAAGGTAAAA CAGAGAGCTC TAAAAATACT
    CGCTCAAAAC TGCTAAATCA TCATGCTTGG GATTATCATT ATGCTGCACA GATACCAAAA
    GATATGTGGA AATCCAAAGA GAAGTCACCA GAAATTATAT CCATTAAGCA AGAGGACACC
    ATTTTGTCTC TGAGGCCTCA TGGAAACAGT CATTCAATAG GGGCAAATGA GAAACAAAAT
    TGGCCTCAAA GAGAAACCAC TTGGGTAAAG CAAGGCCAAA CTCAAAGGAC ATGCTCTCAA
    ATCCCACCAG TGTTGAAACG ACATCAAAGG GAACTTAGTG CTTTTCAATC AGAACAAGAA
    GCAACTGACT ATGATGATGC CATCACCATT GAAACAATCG AGGATTTTGA CATTTACAGT
    GAGGACATAA AGCAAGGTCC CCGCAGCTTT CAACAGAAAA CAAGGCACTA TTTTATTGCA
    GCTGTGGAAC GACTCTGGGA CTATGGGATG AGTACATCTC ATGTTCTACG AAATAGGTAT
    CAAAGTGACA ATGTACCTCA GTTCAAGAAA GTAGTTTTCC AGGAATTTAC TGATGGCTCC
    TTTAGTCAGC CCTTATATCG TGGAGAATTA AATGAACACC TGGGGTTGTT GGGCCCATAT
    ATAAGAGCAG AAGTTGAAGA CAACATTATG GTAACTTTCA AAAACCAGGC CTCCCGTCCC
    TACTCCTTCT ATTCTAGCCT CATTTCTTAT AAAGAAGATC AGAGAGGAGA AGAACCTAGA
    AGAAACTTTG TCAAGCCTAA TGAAACCAAA ATTTATTTTT GGAAAGTACA ACATCATATG
    GCACCCACAG AAGATGAGTT TGACTGCAAG GCCTGGGCTT ATTTCTCTGA TGTTGATCTT
    GAAAGAGATA TGCACTCGGG ATTAATTGGA CCCCTTCTGA TTTGCCACGC GAACACACTG
    AATCCTGCTC ATGGGAGACA AGTGTCAGTA CAGGAATTTG CTCTGCTTTT CACTATCTTT
    GATGAGACCA AGAGCTGGTA CTTCACTGAA AACGTGAAAA GGAACTGCAA GACACCCTGC
    AATTTCCAGA TGGAAGACCC CACTTTGAAA GAGAATTATC GCTTCCATGC AATCAATGGT
    TATGTAATGG ATACCCTACC AGGCTTAGTA ATGGCTCAAG ATCAAAGGAT TCGATGGTAT
    CTTCTCAGCA TGGGCAACAA TGAGAACATC CAATCTATTC ATTTCAGTGG ACATGTTTTC
    ACTGTACGGA AAAAAGAGGA GTATAAAATG GCAGTGTACA ACCTCTACCC AGGTGTTTTT
    GAGACTCTGG AAATGATACC ATCCAGAGCT GGAATATGGC GAGTAGAATG CCTTATTGGC
    GAGCACTTAC AGGCTGGGAT GAGCACTCTT TTTCTGGTGT ACAGCAAGCA GTGTCAGATT
    CCTCTTGGAA TGGCTTCTGG AAGCATCCGT GATTTCCAGA TTACAGCTTC AGGACATTAT
    GGACAGTGGG CCCCAAACCT GGCAAGACTT CATTATTCCG GATCAATCAA TGCCTGGAGT
    ACCAAGGAGC CCTTTTCTTG GATCAAGGTA GATCTGTTGG CACCAATGAT TGTTCATGGC
    ATCAAGACTC AGGGTGCTCG TCAGAAATTT TCCAGCCTTT ATATCTCTCA ATTTATCATC
    ATGTATAGCC TGGATGGGAA GAAGTGGCTG AGTTATCAAG GAAATTCCAC TGGAACCTTA
    ATGGTTTTCT TTGGCAATGT GGACTCATCT GGGATTAAGC ATAATAGTTT TAATCCTCCA
    ATTATTGCTC GATATATCCG TTTGCACCCC ACTCATTCTA GCATCCGTAG TACTCTTCGC
    ATGGAGTTGA TGGGCTGTGA TTTAAACAGT TGCAGCATAC CATTGGGAAT GGAAAGTAAA
    GTAATATCAG ATACACAAAT CACTGCCTCA TCCTACTTCA CCAACATGTT TGCTACTTGG
    TCTCCTTCAC AAGCTCGACT TCACCTCCAG GGAAGGACTA ATGCCTGGCG ACCTCAGGTG
    AATGATCCAA AACAATGGTT GCAAGTGGAC TTACAAAAGA CAATGAAAGT CACTGGAATA
    ATAACCCAGG GAGTGAAATC TCTCTTTACC AGCATGTTTG TGAAAGAGTT CCTTATTTCC
    AGCAGTCAAG ATGGCCATCA CTGGACTCAA ATTTTATACA ATGGCAAGGT AAAGGTTTTT
    CAGGGGAATC AGGACTCATC CACACCTATG ATGAATTCTC TAGACCCACC ATTACTCACT
    CGCTATCTTC GAATTCACCC CCAGATCTGG GAGCACCAAA TTGCTCTGAG GCTTGAGATT
    CTAGGATGTG AGGCCCAGCA GCAATACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001154845.1
    CDS410..7159
    Translation

    Target ORF information:

    RefSeq Version NM_001161373.1
    Organism Mus musculus(house mouse)
    Definition Mus musculus coagulation factor VIII (F8), transcript variant 2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001161373.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    ATGCAAATAG CACTCTTCGC TTGCTTCTTT CTGAGCCTTT TCAATTTCTG CTCTAGTGCC 
    ATCAGAAGAT ACTACCTTGG TGCAGTGGAA TTGTCCTGGA ACTATATTCA GAGTGATCTG
    CTCAGTGTGC TGCATACAGA CTCAAGATTT CTTCCTAGAA TGTCAACATC TTTTCCATTC
    AACACCTCCA TCATGTATAA AAAGACTGTG TTTGTAGAGT ACAAGGACCA GCTTTTCAAC
    ATTGCCAAGC CCAGGCCACC CTGGATGGGT TTGCTAGGTC CTACCATTTG GACTGAGGTT
    CATGACACAG TGGTCATTAC ACTTAAAAAC ATGGCTTCTC ATCCTGTCAG TCTTCATGCT
    GTTGGTGTGT CCTACTGGAA AGCTTCTGAG GGAGATGAAT ATGAAGATCA GACAAGCCAA
    ATGGAGAAGG AAGATGATAA AGTTTTCCCT GGTGAAAGTC ATACTTATGT TTGGCAAGTC
    CTGAAAGAGA ATGGTCCAAT GGCCTCTGAC CCTCCATGTC TCACTTACTC ATATATGTCT
    CATGTGGATC TGGTGAAAGA TTTGAATTCA GGCCTCATTG GAGCTCTGCT AGTATGTAAA
    GAAGGCAGTC TCTCCAAAGA AAGAACACAG ATGTTGTACC AATTTGTACT GCTTTTTGCT
    GTATTTGATG AAGGGAAGAG CTGGCACTCA GAAACAAACG ACTCTTATAC ACAGTCTATG
    GATTCTGCAT CTGCTAGAGA CTGGCCTAAA ATGCACACAG TCAATGGCTA TGTAAACAGG
    TCTCTTCCAG GTCTGATTGG ATGCCATAGG AAATCAGTCT ACTGGCACGT GATTGGAATG
    GGCACCACTC CTGAAATACA CTCAATATTC CTCGAAGGTC ACACATTTTT TGTGAGGAAC
    CACCGTCAAG CTTCATTGGA GATATCACCA ATAACTTTCC TTACTGCTCA AACACTCTTG
    ATAGATCTTG GGCAGTTCCT ACTATTTTGT CATATCTCTT CCCATAAACA TGATGGCATG
    GAAGCTTATG TCAAAGTAGA TAGCTGCCCT GAGGAATCCC AATGGCAAAA GAAAAATAAT
    AATGAGGAAA TGGAAGATTA TGATGATGAT CTTTATTCAG AAATGGATAT GTTCACATTG
    GATTATGACA GCTCTCCTTT TATCCAAATT CGCTCGGTTG CTAAAAAGTA CCCTAAAACT
    TGGATACATT ATATTTCTGC TGAGGAGGAA GACTGGGACT ATGCACCTTC AGTTCCTACC
    TCGGATAATG GAAGTTATAA AAGCCAGTAT CTGAGCAATG GTCCTCATCG GATTGGTAGG
    AAATATAAAA AAGTCAGATT TATAGCATAC ACAGATGAAA CCTTTAAGAC TCGTGAAACT
    ATTCAGCATG AATCAGGACT CTTGGGACCT TTACTTTATG GAGAAGTTGG AGACACACTG
    TTGATTATTT TTAAGAATCA AGCAAGCCGA CCATATAACA TTTACCCTCA TGGAATCACT
    GATGTCAGTC CTCTACATGC AAGGAGATTG CCAAGAGGTA TAAAGCACGT GAAGGATTTG
    CCAATTCATC CAGGAGAGAT ATTCAAGTAC AAGTGGACAG TTACAGTAGA AGATGGACCA
    ACTAAATCAG ATCCACGGTG CCTGACCCGC TATTATTCAA GTTTCATTAA CCCTGAGAGA
    GATCTAGCTT CAGGACTGAT TGGCCCTCTT CTCATCTGCT ACAAAGAATC TGTAGATCAA
    AGGGGAAACC AGATGATGTC AGACAAAAGA AATGTCATCC TGTTTTCTAT ATTTGATGAG
    AACCAAAGCT GGTACATCAC AGAGAACATG CAACGCTTCC TCCCCAATGC AGCTAAAACA
    CAGCCCCAGG ACCCTGGGTT CCAGGCCTCC AACATCATGC ACAGTCTATG GGTCTTGGGG
    TGTCATAATT CAGACTTTCG GAAGAGAGGT ATGACAGCAT TGCTGAAAGT TTCTAGTTGT
    GACAAGAGCA CTAGTGATTA TTATGAAGAA ATATATGAAG ATATTCCAAC ACAGTTGGTG
    AATGAGAACA ATGTCATTGA TCCCAGAAGC TTCTTCCAGA ATACAAATCA TCCTAATACT
    AGGAAAAAGA AATTCAAAGA TTCCACAATT CCAAAAAATG ATATGGAGAA GATTGAGCCT
    CAGTTTGAAG AGATAGCAGA GATGCTTAAA GTACAGAGTG TCTCAGTTAG TGACATGTTG
    ATGCTCTTGG GACAGAGTCA TCCTACTCCA CATGGCTTAT TTTTATCAGA TGGCCAAGAA
    GCCATCTATG AGGCTATTCA TGATGATCAT TCACCAAATG CAATAGACAG CAATGAAGGC
    CCATCTAAAG TGACCCAACT CAGGCCAGAA TCCCATCACA GTGAGAAAAT AGTATTTACT
    CCTCAGCCCG GCCTCCAGTT AAGATCCAAT AAAAGTTTGG AGACAACTAT AGAAGTAAAG
    TGGAAGAAAC TTGGTTTGCA AGTTTCTAGT TTGCCAAGTA ATCTAATGAC TACAACAATT
    CTGTCAGACA ATTTGAAAGC AACTTTTGAA AAGACAGATT CTTCAGGATT TCCAGATATG
    CCAGTTCACT CTAGTAGTAA ATTAAGTACT ACTGCATTTG GTAAGAAAGC ATATTCCCTT
    GTTGGGTCTC ATGTACCTTT AAACGTGAGT GAAGAAAATA GTGATTCCAA CATATTGGAT
    TCAACTTTAA TGTATAGTCA AGAAAGTTTA CCAAGAGATA ATATATTATC AATGGAGAAT
    GATAGATTAC TCAGAGAGAA GAGGTTTCAT GGAATTGCTT TATTGACCAA AGATAATACT
    TTATTCAAAG ACAATGTCTC CTTAATGAAA ACAAACAAAA CATATAATCA TTCAACAACT
    AATGAAAAAC TACACACTGA GAGCCCAACA TCAATTGAGA ATAGTACAAC AGACTTGCAA
    GATGCCATAT TAAAGGTCAA TAGTGAGATT CAAGAAGTAA CAGCTTTGAT TCATGATGGA
    ACACTTTTAG GCAAAAATTC TACATATTTG AGACTAAACC ATATGCTAAA TAGAACTACC
    TCAACAAAAA ATAAAGACAT ATTTCATAGA AAAGATGAAG ATCCTATTCC ACAAGATGAA
    GAGAATACAA TCATGCCATT TTCCAAGATG TTGTTCTTGT CAGAATCTTC AAATTGGTTT
    AAAAAGACCA ATGGAAATAA TTCCTTGAAC TCTGAGCAAG AACATAGTCC AAAGCAATTA
    GTATATTTAA TGTTTAAAAA ATATGTAAAA AATCAAAGTT TCTTGTCAGA GAAAAATAAA
    GTCACAGTAG AACAGGATGG ATTTACAAAG AACATAGGAC TTAAAGACAT GGCTTTTCCA
    CATAATATGA GCATATTTCT TACCACTTTG TCTAACGTAC ATGAAAATGG TAGGCACAAT
    CAAGAAAAAA ATATTCAGGA AGAGATAGAG AAGGAAGCAC TAATTGAAGA GAAAGTAGTT
    TTGCCCCAGG TGCACGAAGC AACTGGCTCT AAGAATTTCT TGAAAGACAT ATTGATACTA
    GGCACTAGGC AAAATATAAG TTTATATGAA GTACATGTAC CAGTACTTCA AAACATCACA
    TCAATAAACA ATTCAACAAA TACAGTACAG ATTCACATGG AGCATTTCTT TAAAAGAAGG
    AAGGACAAGG AAACAAATTC AGAAGGCTTG GTAAATAAAA CCAGAGAAAT GGTAAAAAAC
    TATCCAAGCC AGAAGAATAT TACTACTCAA CGTAGTAAAC GGGCTTTGGG ACAATTCAGA
    CTGTCAACTC AATGGCTTAA AACCATAAAC TGTTCAACAC AGTGTATCAT TAAACAGATA
    GACCACAGCA AGGAAATGAA AAAGTTCATT ACTAAATCTT CCTTATCAGA TTCTTCTGTG
    ATTAAAAGCA CCACTCAGAC AAATAGTTCT GACTCACACA TTGTAAAAAC ATCAGCATTT
    CCACCAATAG ATCTCAAAAG GAGTCCATTC CAAAACAAAT TTTCTCATGT TCAAGCATCA
    TCCTACATTT ATGACTTTAA GACAAAAAGT TCAAGAATTC AAGAAAGCAA TAATTTCTTA
    AAAGAAACCA AAATAAATAA CCCTTCTTTA GCCATTCTAC CATGGAATAT GTTCATAGAT
    CAAGGAAAAT TTACCTCCCC AGGGAAAAGT AACACAAACT CAGTCACATA TAAGAAACGT
    GAGAACATTA TTTTCTTGAA ACCAACTTTG CCTGAAGAAT CTGGCAAAAT TGAATTGCTT
    CCTCAAGTTT CCATTCAAGA GGAAGAAATT TTACCTACAG AAACTAGCCA TGGATCTCCT
    GGACACTTGA ATCTCATGAA AGAGGTCTTT CTTCAGAAAA TACAGGGGCC TACTAAATGG
    AATAAAGCAA AGAGGCATGG AGAAAGTATA AAAGGTAAAA CAGAGAGCTC TAAAAATACT
    CGCTCAAAAC TGCTAAATCA TCATGCTTGG GATTATCATT ATGCTGCACA GATACCAAAA
    GATATGTGGA AATCCAAAGA GAAGTCACCA GAAATTATAT CCATTAAGCA AGAGGACACC
    ATTTTGTCTC TGAGGCCTCA TGGAAACAGT CATTCAATAG GGGCAAATGA GAAACAAAAT
    TGGCCTCAAA GAGAAACCAC TTGGGTAAAG CAAGGCCAAA CTCAAAGGAC ATGCTCTCAA
    ATCCCACCAG TGTTGAAACG ACATCAAAGG GAACTTAGTG CTTTTCAATC AGAACAAGAA
    GCAACTGACT ATGATGATGC CATCACCATT GAAACAATCG AGGATTTTGA CATTTACAGT
    GAGGACATAA AGCAAGGTCC CCGCAGCTTT CAACAGAAAA CAAGGCACTA TTTTATTGCA
    GCTGTGGAAC GACTCTGGGA CTATGGGATG AGTACATCTC ATGTTCTACG AAATAGGTAT
    CAAAGTGACA ATGTACCTCA GTTCAAGAAA GTAGTTTTCC AGGAATTTAC TGATGGCTCC
    TTTAGTCAGC CCTTATATCG TGGAGAATTA AATGAACACC TGGGGTTGTT GGGCCCATAT
    ATAAGAGCAG AAGTTGAAGA CAACATTATG GTAACTTTCA AAAACCAGGC CTCCCGTCCC
    TACTCCTTCT ATTCTAGCCT CATTTCTTAT AAAGAAGATC AGAGAGGAGA AGAACCTAGA
    AGAAACTTTG TCAAGCCTAA TGAAACCAAA ATTTATTTTT GGAAAGTACA ACATCATATG
    GCACCCACAG AAGATGAGTT TGACTGCAAG GCCTGGGCTT ATTTCTCTGA TGTTGATCTT
    GAAAGAGATA TGCACTCGGG ATTAATTGGA CCCCTTCTGA TTTGCCACGC GAACACACTG
    AATCCTGCTC ATGGGAGACA AGTGTCAGTA CAGGAATTTG CTCTGCTTTT CACTATCTTT
    GATGAGACCA AGAGCTGGTA CTTCACTGAA AACGTGAAAA GGAACTGCAA GACACCCTGC
    AATTTCCAGA TGGAAGACCC CACTTTGAAA GAGAATTATC GCTTCCATGC AATCAATGGT
    TATGTAATGG ATACCCTACC AGGCTTAGTA ATGGCTCAAG ATCAAAGGAT TCGATGGTAT
    CTTCTCAGCA TGGGCAACAA TGAGAACATC CAATCTATTC ATTTCAGTGG ACATGTTTTC
    ACTGTACGGA AAAAAGAGGA GTATAAAATG GCAGTGTACA ACCTCTACCC AGGTGTTTTT
    GAGACTCTGG AAATGATACC ATCCAGAGCT GGAATATGGC GAGTAGAATG CCTTATTGGC
    GAGCACTTAC AGGCTGGGAT GAGCACTCTT TTTCTGGTGT ACAGCAAGCA GTGTCAGATT
    CCTCTTGGAA TGGCTTCTGG AAGCATCCGT GATTTCCAGA TTACAGCTTC AGGACATTAT
    GGACAGTGGG CCCCAAACCT GGCAAGACTT CATTATTCCG GATCAATCAA TGCCTGGAGT
    ACCAAGGAGC CCTTTTCTTG GATCAAGGTA GATCTGTTGG CACCAATGAT TGTTCATGGC
    ATCAAGACTC AGGGTGCTCG TCAGAAATTT TCCAGCCTTT ATATCTCTCA ATTTATCATC
    ATGTATAGCC TGGATGGGAA GAAGTGGCTG AGTTATCAAG GAAATTCCAC TGGAACCTTA
    ATGGTTTTCT TTGGCAATGT GGACTCATCT GGGATTAAGC ATAATAGTTT TAATCCTCCA
    ATTATTGCTC GATATATCCG TTTGCACCCC ACTCATTCTA GCATCCGTAG TACTCTTCGC
    ATGGAGTTGA TGGGCTGTGA TTTAAACAGT TGCAGCATAC CATTGGGAAT GGAAAGTAAA
    GTAATATCAG ATACACAAAT CACTGCCTCA TCCTACTTCA CCAACATGTT TGCTACTTGG
    TCTCCTTCAC AAGCTCGACT TCACCTCCAG GGAAGGACTA ATGCCTGGCG ACCTCAGGTG
    AATGATCCAA AACAATGGTT GCAAGTGGAC TTACAAAAGA CAATGAAAGT CACTGGAATA
    ATAACCCAGG GAGTGAAATC TCTCTTTACC AGCATGTTTG TGAAAGAGTT CCTTATTTCC
    AGCAGTCAAG ATGGCCATCA CTGGACTCAA ATTTTATACA ATGGCAAGGT AAAGGTTTTT
    CAGGGGAATC AGGACTCATC CACACCTATG ATGAATTCTC TAGACCCACC ATTACTCACT
    CGCTATCTTC GAATTCACCC CCAGATCTGG GAGCACCAAA TTGCTCTGAG GCTTGAGATT
    CTAGGATGTG AGGCCCAGCA GCAATACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu08137
    Clone ID Related Accession (Same CDS sequence) NM_001161374.1
    Accession Version NM_001161374.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6744bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-10-18
    Organism Mus musculus(house mouse)
    Product coagulation factor VIII isoform 3 precursor
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK051579.1, BC145245.1 and AW489638.1. Transcript Variant: This variant (3) lacks an alternate in-frame exon and uses an alternate in-frame splice junction at the 3' end of an exon compared to variant 1. The resulting isoform (3) has the same N- and C-termini but is shorter compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC145245.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    ATGCAAATAG CACTCTTCGC TTGCTTCTTT CTGAGCCTTT TCAATTTCTG CTCTAGTGCC 
    ATCAGAAGAT ACTACCTTGG TGCAGTGGAA TTGTCCTGGA ACTATATTCA GAGTGATCTG
    CTCAGTGTGC TGCATACAGA CTCAAGATTT CTTCCTAGAA TGTCAACATC TTTTCCATTC
    AACACCTCCA TCATGTATAA AAAGACTGTG TTTGTAGAGT ACAAGGACCA GCTTTTCAAC
    ATTGCCAAGC CCAGGCCACC CTGGATGGGT TTGCTAGGTC CTACCATTTG GACTGAGGTT
    CATGACACAG TGGTCATTAC ACTTAAAAAC ATGGCTTCTC ATCCTGTCAG TCTTCATGCT
    GTTGGTGTGT CCTACTGGAA AGCTTCTGAG GGAGATGAAT ATGAAGATCA GACAAGCCAA
    ATGGAGAAGG AAGATGATAA AGTTTTCCCT GGTGAAAGTC ATACTTATGT TTGGCAAGTC
    CTGAAAGAGA ATGGTCCAAT GGCCTCTGAC CCTCCATGTC TCACTTACTC ATATATGTCT
    CATGTGGATC TGGTGAAAGA TTTGAATTCA GGCCTCATTG GAGCTCTGCT AGTATGTAAA
    GAAGGCAGTC TCTCCAAAGA AAGAACACAG ATGTTGTACC AATTTGTACT GCTTTTTGCT
    GTATTTGATG AAGGGAAGAG CTGGCACTCA GAAACAAACG ACTCTTATAC ACAGTCTATG
    GATTCTGCAT CTGCTAGAGA CTGGCCTAAA ATGCACACAG TCAATGGCTA TGTAAACAGG
    TCTCTTCCAG GTCTGATTGG ATGCCATAGG AAATCAGTCT ACTGGCACGT GATTGGAATG
    GGCACCACTC CTGAAATACA CTCAATATTC CTCGAAGGTC ACACATTTTT TGTGAGGAAC
    CACCGTCAAG CTTCATTGGA GATATCACCA ATAACTTTCC TTACTGCTCA AACACTCTTG
    ATAGATCTTG GGCAGTTCCT ACTATTTTGT CATATCTCTT CCCATAAACA TGATGGCATG
    GAAGCTTATG TCAAAGTAGA TAGCTGCCCT GAGGAATCCC AATGGCAAAA GAAAAATAAT
    AATGAGGAAA TGGAAGATTA TGATGATGAT CTTTATTCAG AAATGGATAT GTTCACATTG
    GATTATGACA GCTCTCCTTT TATCCAAATT CGCTCGGTTG CTAAAAAGTA CCCTAAAACT
    TGGATACATT ATATTTCTGC TGAGGAGGAA GACTGGGACT ATGCACCTTC AGTTCCTACC
    TCGGATAATG GAAGTTATAA AAGCCAGTAT CTGAGCAATG GTCCTCATCG GATTGGTAGG
    AAATATAAAA AAGTCAGATT TATAGCATAC ACAGATGAAA CCTTTAAGAC TCGTGAAACT
    ATTCAGCATG AATCAGGACT CTTGGGACCT TTACTTTATG GAGAAGTTGG AGACACACTG
    TTGATTATTT TTAAGAATCA AGCAAGCCGA CCATATAACA TTTACCCTCA TGGAATCACT
    GATGTCAGTC CTCTACATGC AAGGAGATTG CCAAGAGGTA TAAAGCACGT GAAGGATTTG
    CCAATTCATC CAGGAGAGAT ATTCAAGTAC AAGTGGACAG TTACAGTAGA AGATGGACCA
    ACTAAATCAG ATCCACGGTG CCTGACCCGC TATTATTCAA GTTTCATTAA CCCTGAGAGA
    GATCTAGCTT CAGGACTGAT TGGCCCTCTT CTCATCTGCT ACAAAGAATC TGTAGATCAA
    AGGGGAAACC AGATGATGTC AGACAAAAGA AATGTCATCC TGTTTTCTAT ATTTGATGAG
    AACCAAAGCT GGTACATCAC AGAGAACATG CAACGCTTCC TCCCCAATGC AGCTAAAACA
    CAGCCCCAGG ACCCTGGGTT CCAGGCCTCC AACATCATGC ACAGTCTATG GGTCTTGGGG
    TGTCATAATT CAGACTTTCG GAAGAGAGGT ATGACAGCAT TGCTGAAAGT TTCTAGTTGT
    GACAAGAGCA CTAGTGATTA TTATGAAGAA ATATATGAAG ATATTCCAAC ACAGTTGGTG
    AATGAGAACA ATGTCATTGA TCCCAGAAGC TTCTTCCAGA ATACAAATCA TCCTAATACT
    AGGAAAAAGA AATTCAAAGA TTCCACAATT CCAAAAAATG ATATGGAGAA GATTGAGCCT
    CAGTTTGAAG AGATAGCAGA GATGCTTAAA GTACAGAGTG TCTCAGTTAG TGACATGTTG
    ATGCTCTTGG GACAGAGTCA TCCTACTCCA CATGGCTTAT TTTTATCAGA TGGCCAAGAA
    GCCATCTATG AGGCTATTCA TGATGATCAT TCACCAAATG CAATAGACAG CAATGAAGGC
    CCATCTAAAG TGACCCAACT CAGGCCAGAA TCCCATCACA GTGAGAAAAT AGTATTTACT
    CCTCAGCCCG GCCTCCAGTT AAGATCCAAT AAAAGTTTGG AGACAACTAT AGAAGTAAAG
    TGGAAGAAAC TTGGTTTGCA AGTTTCTAGT TTGCCAAGTA ATCTAATGAC TACAACAATT
    CTGTCAGACA ATTTGAAAGC AACTTTTGAA AAGACAGATT CTTCAGGATT TCCAGATATG
    CCAGTTCACT CTAGTAGTAA ATTAAGTACT ACTGCATTTG GTAAGAAAGC ATATTCCCTT
    GTTGGGTCTC ATGTACCTTT AAACGTGAGT GAAGAAAATA GTGATTCCAA CATATTGGAT
    TCAACTTTAA TGTATAGTCA AGAAAGTTTA CCAAGAGATA ATATATTATC AATGGAGAAT
    GATAGATTAC TCAGAGAGAA GAGGTTTCAT GGAATTGCTT TATTGACCAA AGATAATACT
    TTATTCAAAG ACAATGTCTC CTTAATGAAA ACAAACAAAA CATATAATCA TTCAACAACT
    AATGAAAAAC TACACACTGA GAGCCCAACA TCAATTGAGA ATAGTACAAC AGACTTGCAA
    GATGCCATAT TAAAGGTCAA TAGTGAGATT CAAGAAGTAA CAGCTTTGAT TCATGATGGA
    ACACTTTTAG GCAAAAATTC TACATATTTG AGACTAAACC ATATGCTAAA TAGAACTACC
    TCAACAAAAA ATAAAGACAT ATTTCATAGA AAAGATGAAG ATCCTATTCC ACAAGATGAA
    GAGAATACAA TCATGCCATT TTCCAAGATG TTGTTCTTGT CAGAATCTTC AAATTGGTTT
    AAAAAGACCA ATGGAAATAA TTCCTTGAAC TCTGAGCAAG AACATAGTCC AAAGCAATTA
    GTATATTTAA TGTTTAAAAA ATATGTAAAA AATCAAAGTT TCTTGTCAGA GAAAAATAAA
    GTCACAGTAG AACAGGATGG ATTTACAAAG AACATAGGAC TTAAAGACAT GGCTTTTCCA
    CATAATATGA GCATATTTCT TACCACTTTG TCTAACGTAC ATGAAAATGG TAGGCACAAT
    CAAGAAAAAA ATATTCAGGA AGAGATAGAG AAGGAAGCAC TAATTGAAGA GAAAGTAGTT
    TTGCCCCAGG TGCACGAAGC AACTGGCTCT AAGAATTTCT TGAAAGACAT ATTGATACTA
    GGCACTAGGC AAAATATAAG TTTATATGAA GTACATGTAC CAGTACTTCA AAACATCACA
    TCAATAAACA ATTCAACAAA TACAGTACAG ATTCACATGG AGCATTTCTT TAAAAGAAGG
    AAGGACAAGG AAACAAATTC AGAAGGCTTG GTAAATAAAA CCAGAGAAAT GGTAAAAAAC
    TATCCAAGCC AGAAGAATAT TACTACTCAA CGTAGTAAAC GGGCTTTGGG ACAATTCAGA
    CTGTCAACTC AATGGCTTAA AACCATAAAC TGTTCAACAC AGTGTATCAT TAAACAGATA
    GACCACAGCA AGGAAATGAA AAAGTTCATT ACTAAATCTT CCTTATCAGA TTCTTCTGTG
    ATTAAAAGCA CCACTCAGAC AAATAGTTCT GACTCACACA TTGTAAAAAC ATCAGCATTT
    CCACCAATAG ATCTCAAAAG GAGTCCATTC CAAAACAAAT TTTCTCATGT TCAAGCATCA
    TCCTACATTT ATGACTTTAA GACAAAAAGT TCAAGAATTC AAGAAAGCAA TAATTTCTTA
    AAAGAAACCA AAATAAATAA CCCTTCTTTA GCCATTCTAC CATGGAATAT GTTCATAGAT
    CAAGGAAAAT TTACCTCCCC AGGGAAAAGT AACACAAACT CAGTCACATA TAAGAAACGT
    GAGAACATTA TTTTCTTGAA ACCAACTTTG CCTGAAGAAT CTGGCAAAAT TGAATTGCTT
    CCTCAAGTTT CCATTCAAGA GGAAGAAATT TTACCTACAG AAACTAGCCA TGGATCTCCT
    GGACACTTGA ATCTCATGAA AGAGGTCTTT CTTCAGAAAA TACAGGGGCC TACTAAATGG
    AATAAAGCAA AGAGGCATGG AGAAAGTATA AAAGGTAAAA CAGAGAGCTC TAAAAATACT
    CGCTCAAAAC TGCTAAATCA TCATGCTTGG GATTATCATT ATGCTGCACA GATACCAAAA
    GATATGTGGA AATCCAAAGA GAAGTCACCA GAAATTATAT CCATTAAGCA AGAGGACACC
    ATTTTGTCTC TGAGGCCTCA TGGAAACAGT CATTCAATAG GGGCAAATGA GAAACAAAAT
    TGGCCTCAAA GAGAAACCAC TTGGGTAAAG CAAGGCCAAA CTCAAAGGAC ATGCTCTCAA
    ATCCCACCAG TGTTGAAACG ACATCAAAGG GAACTTAGTG CTTTTCAATC AGAACAAGAA
    GCAACTGACT ATGATGATGC CATCACCATT GAAACAATCG AGGATTTTGA CATTTACAGT
    GAGGACATAA AGCAAGGTCC CCGCAGCTTT CAACAGAAAA CAAGGCACTA TTTTATTGCA
    GCTGTGGAAC GACTCTGGGA CTATGGGATG AGTACATCTC ATGTTCTACG AAATAGGTAT
    CAAAGTGACA ATGTACCTCA GTTCAAGAAA GTAGTTTTCC AGGAATTTAC TGATGGCTCC
    TTTAGTCAGC CCTTATATCG TGGAGAATTA AATGAACACC TGGGGTTGTT GGGCCCATAT
    ATAAGAGCAG AAGTTGAAGA CAACATTATG GTAACTTTCA AAAACCAGGC CTCCCGTCCC
    TACTCCTTCT ATTCTAGCCT CATTTCTTAT AAAGAAGATC AGAGAGGAGA AGAACCTAGA
    AGAAACTTTG TCAAGCCTAA TGAAACCAAA ATTTATTTTT GGAAAGTACA ACATCATATG
    GCACCCACAG AAGATGAGTT TGACTGCAAG GCCTGGGCTT ATTTCTCTGA TGTTGATCTT
    GAAAGAGATA TGCACTCGGG ATTAATTGGA CCCCTTCTGA TTTGCCACGC GAACACACTG
    AATCCTGCTC ATGGGAGACA AGTGTCAGTA CAGGAATTTG CTCTGCTTTT CACTATCTTT
    GATGAGACCA AGAGCTGGTA CTTCACTGAA AACGTGAAAA GGAACTGCAA GACACCCTGC
    AATTTCCAGA TGGAAGACCC CACTTTGAAA GAGAATTATC GCTTCCATGC AATCAATGGT
    TATGTAATGG ATACCCTACC AGGCTTAGTA ATGGCTCAAG ATCAAAGGAT TCGATGGTAT
    CTTCTCAGCA TGGGCAACAA TGAGAACATC CAATCTATTC ATTTCAGTGG ACATGTTTTC
    ACTGTACGGA AAAAAGAGGA GTATAAAATG GCAGTGTACA ACCTCTACCC AGGTGTTTTT
    GAGACTCTGG AAATGATACC ATCCAGAGCT GGAATATGGC GAGTAGAATG CCTTATTGGC
    GAGCACTTAC AGGCTGGGAT GAGCACTCTT TTTCTGGTGT ACAGCAAGCA GTGTCAGATT
    CCTCTTGGAA TGGCTTCTGG AAGCATCCGT GATTTCCAGA TTACAGCTTC AGGACATTAT
    GGACAGTGGG CCCCAAACCT GGCAAGACTT CATTATTCCG GATCAATCAA TGCCTGGAGT
    ACCAAGGAGC CCTTTTCTTG GATCAAGGTA GATCTGTTGG CACCAATGAT TGTTCATGGC
    ATCAAGACTC AGGGTGCTCG TCAGAAATTT TCCAGCCTTT ATATCTCTCA ATTTATCATC
    ATGTATAGCC TGGATGGGAA GAAGTGGCTG AGTTATCAAG GAAATTCCAC TGGAACCTTA
    ATGGTTTTCT TTGGCAATGT GGACTCATCT GGGATTAAGC ATAATAGTTT TAATCCTCCA
    ATTATTGCTC GATATATCCG TTTGCACCCC ACTCATTCTA GCATCCGTAG TACTCTTCGC
    ATGGAGTTGA TGGGCTGTGA TTTAAACAGT TGCAGCATAC CATTGGGAAT GGAAAGTAAA
    GTAATATCAG ATACACAAAT CACTGCCTCA TCCTACTTCA CCAACATGTT TGCTACTTGG
    TCTCCTTCAC AAGCTCGACT TCACCTCCAG GGAAGGACTA ATGCCTGGCG ACCTCAGGTG
    AATGATCCAA AACAATGGTT GCAAGTGGAC TTACAAAAGA CAATGAAAGT CACTGGAATA
    ATAACCCAGG GAGTGAAATC TCTCTTTACC AGCATGTTTG TGAAAGAGTT CCTTATTTCC
    AGCAGTCAAG ATGGCCATCA CTGGACTCAA ATTTTATACA ATGGCAAGGT TTTTCAGGGG
    AATCAGGACT CATCCACACC TATGATGAAT TCTCTAGACC CACCATTACT CACTCGCTAT
    CTTCGAATTC ACCCCCAGAT CTGGGAGCAC CAAATTGCTC TGAGGCTTGA GATTCTAGGA
    TGTGAGGCCC AGCAGCAATA CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001154846.1
    CDS410..7153
    Translation

    Target ORF information:

    RefSeq Version NM_001161374.1
    Organism Mus musculus(house mouse)
    Definition Mus musculus coagulation factor VIII (F8), transcript variant 3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001161374.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    ATGCAAATAG CACTCTTCGC TTGCTTCTTT CTGAGCCTTT TCAATTTCTG CTCTAGTGCC 
    ATCAGAAGAT ACTACCTTGG TGCAGTGGAA TTGTCCTGGA ACTATATTCA GAGTGATCTG
    CTCAGTGTGC TGCATACAGA CTCAAGATTT CTTCCTAGAA TGTCAACATC TTTTCCATTC
    AACACCTCCA TCATGTATAA AAAGACTGTG TTTGTAGAGT ACAAGGACCA GCTTTTCAAC
    ATTGCCAAGC CCAGGCCACC CTGGATGGGT TTGCTAGGTC CTACCATTTG GACTGAGGTT
    CATGACACAG TGGTCATTAC ACTTAAAAAC ATGGCTTCTC ATCCTGTCAG TCTTCATGCT
    GTTGGTGTGT CCTACTGGAA AGCTTCTGAG GGAGATGAAT ATGAAGATCA GACAAGCCAA
    ATGGAGAAGG AAGATGATAA AGTTTTCCCT GGTGAAAGTC ATACTTATGT TTGGCAAGTC
    CTGAAAGAGA ATGGTCCAAT GGCCTCTGAC CCTCCATGTC TCACTTACTC ATATATGTCT
    CATGTGGATC TGGTGAAAGA TTTGAATTCA GGCCTCATTG GAGCTCTGCT AGTATGTAAA
    GAAGGCAGTC TCTCCAAAGA AAGAACACAG ATGTTGTACC AATTTGTACT GCTTTTTGCT
    GTATTTGATG AAGGGAAGAG CTGGCACTCA GAAACAAACG ACTCTTATAC ACAGTCTATG
    GATTCTGCAT CTGCTAGAGA CTGGCCTAAA ATGCACACAG TCAATGGCTA TGTAAACAGG
    TCTCTTCCAG GTCTGATTGG ATGCCATAGG AAATCAGTCT ACTGGCACGT GATTGGAATG
    GGCACCACTC CTGAAATACA CTCAATATTC CTCGAAGGTC ACACATTTTT TGTGAGGAAC
    CACCGTCAAG CTTCATTGGA GATATCACCA ATAACTTTCC TTACTGCTCA AACACTCTTG
    ATAGATCTTG GGCAGTTCCT ACTATTTTGT CATATCTCTT CCCATAAACA TGATGGCATG
    GAAGCTTATG TCAAAGTAGA TAGCTGCCCT GAGGAATCCC AATGGCAAAA GAAAAATAAT
    AATGAGGAAA TGGAAGATTA TGATGATGAT CTTTATTCAG AAATGGATAT GTTCACATTG
    GATTATGACA GCTCTCCTTT TATCCAAATT CGCTCGGTTG CTAAAAAGTA CCCTAAAACT
    TGGATACATT ATATTTCTGC TGAGGAGGAA GACTGGGACT ATGCACCTTC AGTTCCTACC
    TCGGATAATG GAAGTTATAA AAGCCAGTAT CTGAGCAATG GTCCTCATCG GATTGGTAGG
    AAATATAAAA AAGTCAGATT TATAGCATAC ACAGATGAAA CCTTTAAGAC TCGTGAAACT
    ATTCAGCATG AATCAGGACT CTTGGGACCT TTACTTTATG GAGAAGTTGG AGACACACTG
    TTGATTATTT TTAAGAATCA AGCAAGCCGA CCATATAACA TTTACCCTCA TGGAATCACT
    GATGTCAGTC CTCTACATGC AAGGAGATTG CCAAGAGGTA TAAAGCACGT GAAGGATTTG
    CCAATTCATC CAGGAGAGAT ATTCAAGTAC AAGTGGACAG TTACAGTAGA AGATGGACCA
    ACTAAATCAG ATCCACGGTG CCTGACCCGC TATTATTCAA GTTTCATTAA CCCTGAGAGA
    GATCTAGCTT CAGGACTGAT TGGCCCTCTT CTCATCTGCT ACAAAGAATC TGTAGATCAA
    AGGGGAAACC AGATGATGTC AGACAAAAGA AATGTCATCC TGTTTTCTAT ATTTGATGAG
    AACCAAAGCT GGTACATCAC AGAGAACATG CAACGCTTCC TCCCCAATGC AGCTAAAACA
    CAGCCCCAGG ACCCTGGGTT CCAGGCCTCC AACATCATGC ACAGTCTATG GGTCTTGGGG
    TGTCATAATT CAGACTTTCG GAAGAGAGGT ATGACAGCAT TGCTGAAAGT TTCTAGTTGT
    GACAAGAGCA CTAGTGATTA TTATGAAGAA ATATATGAAG ATATTCCAAC ACAGTTGGTG
    AATGAGAACA ATGTCATTGA TCCCAGAAGC TTCTTCCAGA ATACAAATCA TCCTAATACT
    AGGAAAAAGA AATTCAAAGA TTCCACAATT CCAAAAAATG ATATGGAGAA GATTGAGCCT
    CAGTTTGAAG AGATAGCAGA GATGCTTAAA GTACAGAGTG TCTCAGTTAG TGACATGTTG
    ATGCTCTTGG GACAGAGTCA TCCTACTCCA CATGGCTTAT TTTTATCAGA TGGCCAAGAA
    GCCATCTATG AGGCTATTCA TGATGATCAT TCACCAAATG CAATAGACAG CAATGAAGGC
    CCATCTAAAG TGACCCAACT CAGGCCAGAA TCCCATCACA GTGAGAAAAT AGTATTTACT
    CCTCAGCCCG GCCTCCAGTT AAGATCCAAT AAAAGTTTGG AGACAACTAT AGAAGTAAAG
    TGGAAGAAAC TTGGTTTGCA AGTTTCTAGT TTGCCAAGTA ATCTAATGAC TACAACAATT
    CTGTCAGACA ATTTGAAAGC AACTTTTGAA AAGACAGATT CTTCAGGATT TCCAGATATG
    CCAGTTCACT CTAGTAGTAA ATTAAGTACT ACTGCATTTG GTAAGAAAGC ATATTCCCTT
    GTTGGGTCTC ATGTACCTTT AAACGTGAGT GAAGAAAATA GTGATTCCAA CATATTGGAT
    TCAACTTTAA TGTATAGTCA AGAAAGTTTA CCAAGAGATA ATATATTATC AATGGAGAAT
    GATAGATTAC TCAGAGAGAA GAGGTTTCAT GGAATTGCTT TATTGACCAA AGATAATACT
    TTATTCAAAG ACAATGTCTC CTTAATGAAA ACAAACAAAA CATATAATCA TTCAACAACT
    AATGAAAAAC TACACACTGA GAGCCCAACA TCAATTGAGA ATAGTACAAC AGACTTGCAA
    GATGCCATAT TAAAGGTCAA TAGTGAGATT CAAGAAGTAA CAGCTTTGAT TCATGATGGA
    ACACTTTTAG GCAAAAATTC TACATATTTG AGACTAAACC ATATGCTAAA TAGAACTACC
    TCAACAAAAA ATAAAGACAT ATTTCATAGA AAAGATGAAG ATCCTATTCC ACAAGATGAA
    GAGAATACAA TCATGCCATT TTCCAAGATG TTGTTCTTGT CAGAATCTTC AAATTGGTTT
    AAAAAGACCA ATGGAAATAA TTCCTTGAAC TCTGAGCAAG AACATAGTCC AAAGCAATTA
    GTATATTTAA TGTTTAAAAA ATATGTAAAA AATCAAAGTT TCTTGTCAGA GAAAAATAAA
    GTCACAGTAG AACAGGATGG ATTTACAAAG AACATAGGAC TTAAAGACAT GGCTTTTCCA
    CATAATATGA GCATATTTCT TACCACTTTG TCTAACGTAC ATGAAAATGG TAGGCACAAT
    CAAGAAAAAA ATATTCAGGA AGAGATAGAG AAGGAAGCAC TAATTGAAGA GAAAGTAGTT
    TTGCCCCAGG TGCACGAAGC AACTGGCTCT AAGAATTTCT TGAAAGACAT ATTGATACTA
    GGCACTAGGC AAAATATAAG TTTATATGAA GTACATGTAC CAGTACTTCA AAACATCACA
    TCAATAAACA ATTCAACAAA TACAGTACAG ATTCACATGG AGCATTTCTT TAAAAGAAGG
    AAGGACAAGG AAACAAATTC AGAAGGCTTG GTAAATAAAA CCAGAGAAAT GGTAAAAAAC
    TATCCAAGCC AGAAGAATAT TACTACTCAA CGTAGTAAAC GGGCTTTGGG ACAATTCAGA
    CTGTCAACTC AATGGCTTAA AACCATAAAC TGTTCAACAC AGTGTATCAT TAAACAGATA
    GACCACAGCA AGGAAATGAA AAAGTTCATT ACTAAATCTT CCTTATCAGA TTCTTCTGTG
    ATTAAAAGCA CCACTCAGAC AAATAGTTCT GACTCACACA TTGTAAAAAC ATCAGCATTT
    CCACCAATAG ATCTCAAAAG GAGTCCATTC CAAAACAAAT TTTCTCATGT TCAAGCATCA
    TCCTACATTT ATGACTTTAA GACAAAAAGT TCAAGAATTC AAGAAAGCAA TAATTTCTTA
    AAAGAAACCA AAATAAATAA CCCTTCTTTA GCCATTCTAC CATGGAATAT GTTCATAGAT
    CAAGGAAAAT TTACCTCCCC AGGGAAAAGT AACACAAACT CAGTCACATA TAAGAAACGT
    GAGAACATTA TTTTCTTGAA ACCAACTTTG CCTGAAGAAT CTGGCAAAAT TGAATTGCTT
    CCTCAAGTTT CCATTCAAGA GGAAGAAATT TTACCTACAG AAACTAGCCA TGGATCTCCT
    GGACACTTGA ATCTCATGAA AGAGGTCTTT CTTCAGAAAA TACAGGGGCC TACTAAATGG
    AATAAAGCAA AGAGGCATGG AGAAAGTATA AAAGGTAAAA CAGAGAGCTC TAAAAATACT
    CGCTCAAAAC TGCTAAATCA TCATGCTTGG GATTATCATT ATGCTGCACA GATACCAAAA
    GATATGTGGA AATCCAAAGA GAAGTCACCA GAAATTATAT CCATTAAGCA AGAGGACACC
    ATTTTGTCTC TGAGGCCTCA TGGAAACAGT CATTCAATAG GGGCAAATGA GAAACAAAAT
    TGGCCTCAAA GAGAAACCAC TTGGGTAAAG CAAGGCCAAA CTCAAAGGAC ATGCTCTCAA
    ATCCCACCAG TGTTGAAACG ACATCAAAGG GAACTTAGTG CTTTTCAATC AGAACAAGAA
    GCAACTGACT ATGATGATGC CATCACCATT GAAACAATCG AGGATTTTGA CATTTACAGT
    GAGGACATAA AGCAAGGTCC CCGCAGCTTT CAACAGAAAA CAAGGCACTA TTTTATTGCA
    GCTGTGGAAC GACTCTGGGA CTATGGGATG AGTACATCTC ATGTTCTACG AAATAGGTAT
    CAAAGTGACA ATGTACCTCA GTTCAAGAAA GTAGTTTTCC AGGAATTTAC TGATGGCTCC
    TTTAGTCAGC CCTTATATCG TGGAGAATTA AATGAACACC TGGGGTTGTT GGGCCCATAT
    ATAAGAGCAG AAGTTGAAGA CAACATTATG GTAACTTTCA AAAACCAGGC CTCCCGTCCC
    TACTCCTTCT ATTCTAGCCT CATTTCTTAT AAAGAAGATC AGAGAGGAGA AGAACCTAGA
    AGAAACTTTG TCAAGCCTAA TGAAACCAAA ATTTATTTTT GGAAAGTACA ACATCATATG
    GCACCCACAG AAGATGAGTT TGACTGCAAG GCCTGGGCTT ATTTCTCTGA TGTTGATCTT
    GAAAGAGATA TGCACTCGGG ATTAATTGGA CCCCTTCTGA TTTGCCACGC GAACACACTG
    AATCCTGCTC ATGGGAGACA AGTGTCAGTA CAGGAATTTG CTCTGCTTTT CACTATCTTT
    GATGAGACCA AGAGCTGGTA CTTCACTGAA AACGTGAAAA GGAACTGCAA GACACCCTGC
    AATTTCCAGA TGGAAGACCC CACTTTGAAA GAGAATTATC GCTTCCATGC AATCAATGGT
    TATGTAATGG ATACCCTACC AGGCTTAGTA ATGGCTCAAG ATCAAAGGAT TCGATGGTAT
    CTTCTCAGCA TGGGCAACAA TGAGAACATC CAATCTATTC ATTTCAGTGG ACATGTTTTC
    ACTGTACGGA AAAAAGAGGA GTATAAAATG GCAGTGTACA ACCTCTACCC AGGTGTTTTT
    GAGACTCTGG AAATGATACC ATCCAGAGCT GGAATATGGC GAGTAGAATG CCTTATTGGC
    GAGCACTTAC AGGCTGGGAT GAGCACTCTT TTTCTGGTGT ACAGCAAGCA GTGTCAGATT
    CCTCTTGGAA TGGCTTCTGG AAGCATCCGT GATTTCCAGA TTACAGCTTC AGGACATTAT
    GGACAGTGGG CCCCAAACCT GGCAAGACTT CATTATTCCG GATCAATCAA TGCCTGGAGT
    ACCAAGGAGC CCTTTTCTTG GATCAAGGTA GATCTGTTGG CACCAATGAT TGTTCATGGC
    ATCAAGACTC AGGGTGCTCG TCAGAAATTT TCCAGCCTTT ATATCTCTCA ATTTATCATC
    ATGTATAGCC TGGATGGGAA GAAGTGGCTG AGTTATCAAG GAAATTCCAC TGGAACCTTA
    ATGGTTTTCT TTGGCAATGT GGACTCATCT GGGATTAAGC ATAATAGTTT TAATCCTCCA
    ATTATTGCTC GATATATCCG TTTGCACCCC ACTCATTCTA GCATCCGTAG TACTCTTCGC
    ATGGAGTTGA TGGGCTGTGA TTTAAACAGT TGCAGCATAC CATTGGGAAT GGAAAGTAAA
    GTAATATCAG ATACACAAAT CACTGCCTCA TCCTACTTCA CCAACATGTT TGCTACTTGG
    TCTCCTTCAC AAGCTCGACT TCACCTCCAG GGAAGGACTA ATGCCTGGCG ACCTCAGGTG
    AATGATCCAA AACAATGGTT GCAAGTGGAC TTACAAAAGA CAATGAAAGT CACTGGAATA
    ATAACCCAGG GAGTGAAATC TCTCTTTACC AGCATGTTTG TGAAAGAGTT CCTTATTTCC
    AGCAGTCAAG ATGGCCATCA CTGGACTCAA ATTTTATACA ATGGCAAGGT TTTTCAGGGG
    AATCAGGACT CATCCACACC TATGATGAAT TCTCTAGACC CACCATTACT CACTCGCTAT
    CTTCGAATTC ACCCCCAGAT CTGGGAGCAC CAAATTGCTC TGAGGCTTGA GATTCTAGGA
    TGTGAGGCCC AGCAGCAATA CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu56128
    Clone ID Related Accession (Same CDS sequence) XM_006527789.3 , XM_006527789.4
    Accession Version XM_006527789.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6954bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-21
    Organism Mus musculus(house mouse)
    Product coagulation factor VIII isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000086.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006527789.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    ATGCAAATAG CACTCTTCGC TTGCTTCTTT CTGAGCCTTT TCAATTTCTG CTCTAGTGCC 
    ATCAGAAGAT ACTACCTTGG TGCAGTGGAA TTGTCCTGGA ACTATATTCA GAGTGATCTG
    CTCAGTGTGC TGCATACAGA CTCAAGATTT CTTCCTAGAA TGTCAACATC TTTTCCATTC
    AACACCTCCA TCATGTATAA AAAGACTGTG TTTGTAGAGT ACAAGGACCA GCTTTTCAAC
    ATTGCCAAGC CCAGGCCACC CTGGATGGGT TTGCTAGGTC CTACCATTTG GACTGAGGTT
    CATGACACAG TGGTCATTAC ACTTAAAAAC ATGGCTTCTC ATCCTGTCAG TCTTCATGCT
    GTTGGTGTGT CCTACTGGAA AGCTTCTGAG GGAGATGAAT ATGAAGATCA GACAAGCCAA
    ATGGAGAAGG AAGATGATAA AGTTTTCCCT GGTGAAAGTC ATACTTATGT TTGGCAAGTC
    CTGAAAGAGA ATGGTCCAAT GGCCTCTGAC CCTCCATGTC TCACTTACTC ATATATGTCT
    CATGTGGATC TGGTGAAAGA TTTGAATTCA GGCCTCATTG GAGCTCTGCT AGTATGTAAA
    GAAGGCAGTC TCTCCAAAGA AAGAACACAG ATGTTGTACC AATTTGTACT GCTTTTTGCT
    GTATTTGATG AAGGGAAGAG CTGGCACTCA GAAACAAACG ACTCTTATAC ACAGTCTATG
    GATTCTGCAT CTGCTAGAGA CTGGCCTAAA ATGCACACAG TCAATGGCTA TGTAAACAGG
    TCTCTTCCAG GTCTGATTGG ATGCCATAGG AAATCAGTCT ACTGGCACGT GATTGGAATG
    GGCACCACTC CTGAAATACA CTCAATATTC CTCGAAGGTC ACACATTTTT TGTGAGGAAC
    CACCGTCAAG CTTCATTGGA GATATCACCA ATAACTTTCC TTACTGCTCA AACACTCTTG
    ATAGATCTTG GGCAGTTCCT ACTATTTTGT CATATCTCTT CCCATAAACA TGATGGCATG
    GAAGCTTATG TCAAAGTAGA TAGCTGCCCT GAGGAATCCC AATGGCAAAA GAAAAATAAT
    AATGAGGAAA TGGAAGATTA TGATGATGAT CTTTATTCAG AAATGGATAT GTTCACATTG
    GATTATGACA GCTCTCCTTT TATCCAAATT CGCTCGGTTG CTAAAAAGTA CCCTAAAACT
    TGGATACATT ATATTTCTGC TGAGGAGGAA GACTGGGACT ATGCACCTTC AGTTCCTACC
    TCGGATAATG GAAGTTATAA AAGCCAGTAT CTGAGCAATG GTCCTCATCG GATTGGTAGG
    AAATATAAAA AAGTCAGATT TATAGCATAC ACAGATGAAA CCTTTAAGAC TCGTGAAACT
    ATTCAGCATG AATCAGGACT CTTGGGACCT TTACTTTATG GAGAAGTTGG AGACACACTG
    TTGATTATTT TTAAGAATCA AGCAAGCCGA CCATATAACA TTTACCCTCA TGGAATCACT
    GATGTCAGTC CTCTACATGC AAGGAGATTG CCAAGAGGTA TAAAGCACGT GAAGGATTTG
    CCAATTCATC CAGGAGAGAT ATTCAAGTAC AAGTGGACAG TTACAGTAGA AGATGGACCA
    ACTAAATCAG ATCCACGGTG CCTGACCCGC TATTATTCAA GTTTCATTAA CCCTGAGAGA
    GATCTAGCTT CAGGACTGAT TGGCCCTCTT CTCATCTGCT ACAAAGAATC TGTAGATCAA
    AGGGGAAACC AGATGATGTC AGACAAAAGA AATGTCATCC TGTTTTCTAT ATTTGATGAG
    AACCAAAGCT GGTACATCAC AGAGAACATG CAACGCTTCC TCCCCAATGC AGCTAAAACA
    CAGCCCCAGG ACCCTGGGTT CCAGGCCTCC AACATCATGC ACAGCATCAA TGGCTATGTT
    TTTGATAGCT TGGAGTTGAC AGTTTGTTTG CATGAGGTGG CATACTGGCA CATTCTCAGT
    GTTGGAGCAC AGACAGACTT CTTATCTATC TTCTTCTCTG GATATACTTT CAAACACAAA
    ATGGTCTATG AAGATACACT TACCCTGTTC CCATTCTCAG GAGAAACTGT CTTTATGTCG
    ATGGAAAACC CAGGTCTATG GGTCTTGGGG TGTCATAATT CAGACTTTCG GAAGAGAGGT
    ATGACAGCAT TGCTGAAAGT TTCTAGTTGT GACAAGAGCA CTAGTGATTA TTATGAAGAA
    ATATATGAAG ATATTCCAAC ACAGTTGGTG AATGAGAACA ATGTCATTGA TCCCAGAAGC
    TTCTTCCAGA ATACAAATCA TCCTAATACT AGGAAAAAGA AATTCAAAGA TTCCACAATT
    CCAAAAAATG ATATGGAGAA GATTGAGCCT CAGTTTGAAG AGATAGCAGA GATGCTTAAA
    GTACAGAGTG TCTCAGTTAG TGACATGTTG ATGCTCTTGG GACAGAGTCA TCCTACTCCA
    CATGGCTTAT TTTTATCAGA TGGCCAAGAA GCCATCTATG AGGCTATTCA TGATGATCAT
    TCACCAAATG CAATAGACAG CAATGAAGGC CCATCTAAAG TGACCCAACT CAGGCCAGAA
    TCCCATCACA GTGAGAAAAT AGTATTTACT CCTCAGCCCG GCCTCCAGTT AAGATCCAAT
    AAAAGTTTGG AGACAACTAT AGAAGTAAAG TGGAAGAAAC TTGGTTTGCA AGTTTCTAGT
    TTGCCAAGTA ATCTAATGAC TACAACAATT CTGTCAGACA ATTTGAAAGC AACTTTTGAA
    AAGACAGATT CTTCAGGATT TCCAGATATG CCAGTTCACT CTAGTAGTAA ATTAAGTACT
    ACTGCATTTG GTAAGAAAGC ATATTCCCTT GTTGGGTCTC ATGTACCTTT AAACGTGAGT
    GAAGAAAATA GTGATTCCAA CATATTGGAT TCAACTTTAA TGTATAGTCA AGAAAGTTTA
    CCAAGAGATA ATATATTATC AATGGAGAAT GATAGATTAC TCAGAGAGAA GAGGTTTCAT
    GGAATTGCTT TATTGACCAA AGATAATACT TTATTCAAAG ACAATGTCTC CTTAATGAAA
    ACAAACAAAA CATATAATCA TTCAACAACT AATGAAAAAC TACACACTGA GAGCCCAACA
    TCAATTGAGA ATAGTACAAC AGACTTGCAA GATGCCATAT TAAAGGTCAA TAGTGAGATT
    CAAGAAGTAA CAGCTTTGAT TCATGATGGA ACACTTTTAG GCAAAAATTC TACATATTTG
    AGACTAAACC ATATGCTAAA TAGAACTACC TCAACAAAAA ATAAAGACAT ATTTCATAGA
    AAAGATGAAG ATCCTATTCC ACAAGATGAA GAGAATACAA TCATGCCATT TTCCAAGATG
    TTGTTCTTGT CAGAATCTTC AAATTGGTTT AAAAAGACCA ATGGAAATAA TTCCTTGAAC
    TCTGAGCAAG AACATAGTCC AAAGCAATTA GTATATTTAA TGTTTAAAAA ATATGTAAAA
    AATCAAAGTT TCTTGTCAGA GAAAAATAAA GTCACAGTAG AACAGGATGG ATTTACAAAG
    AACATAGGAC TTAAAGACAT GGCTTTTCCA CATAATATGA GCATATTTCT TACCACTTTG
    TCTAACGTAC ATGAAAATGG TAGGCACAAT CAAGAAAAAA ATATTCAGGA AGAGATAGAG
    AAGGAAGCAC TAATTGAAGA GAAAGTAGTT TTGCCCCAGG TGCACGAAGC AACTGGCTCT
    AAGAATTTCT TGAAAGACAT ATTGATACTA GGCACTAGGC AAAATATAAG TTTATATGAA
    GTACATGTAC CAGTACTTCA AAACATCACA TCAATAAACA ATTCAACAAA TACAGTACAG
    ATTCACATGG AGCATTTCTT TAAAAGAAGG AAGGACAAGG AAACAAATTC AGAAGGCTTG
    GTAAATAAAA CCAGAGAAAT GGTAAAAAAC TATCCAAGCC AGAAGAATAT TACTACTCAA
    CGTAGTAAAC GGGCTTTGGG ACAATTCAGA CTGTCAACTC AATGGCTTAA AACCATAAAC
    TGTTCAACAC AGTGTATCAT TAAACAGATA GACCACAGCA AGGAAATGAA AAAGTTCATT
    ACTAAATCTT CCTTATCAGA TTCTTCTGTG ATTAAAAGCA CCACTCAGAC AAATAGTTCT
    GACTCACACA TTGTAAAAAC ATCAGCATTT CCACCAATAG ATCTCAAAAG GAGTCCATTC
    CAAAACAAAT TTTCTCATGT TCAAGCATCA TCCTACATTT ATGACTTTAA GACAAAAAGT
    TCAAGAATTC AAGAAAGCAA TAATTTCTTA AAAGAAACCA AAATAAATAA CCCTTCTTTA
    GCCATTCTAC CATGGAATAT GTTCATAGAT CAAGGAAAAT TTACCTCCCC AGGGAAAAGT
    AACACAAACT CAGTCACATA TAAGAAACGT GAGAACATTA TTTTCTTGAA ACCAACTTTG
    CCTGAAGAAT CTGGCAAAAT TGAATTGCTT CCTCAAGTTT CCATTCAAGA GGAAGAAATT
    TTACCTACAG AAACTAGCCA TGGATCTCCT GGACACTTGA ATCTCATGAA AGAGGTCTTT
    CTTCAGAAAA TACAGGGGCC TACTAAATGG AATAAAGCAA AGAGGCATGG AGAAAGTATA
    AAAGGTAAAA CAGAGAGCTC TAAAAATACT CGCTCAAAAC TGCTAAATCA TCATGCTTGG
    GATTATCATT ATGCTGCACA GATACCAAAA GATATGTGGA AATCCAAAGA GAAGTCACCA
    GAAATTATAT CCATTAAGCA AGAGGACACC ATTTTGTCTC TGAGGCCTCA TGGAAACAGT
    CATTCAATAG GGGCAAATGA GAAACAAAAT TGGCCTCAAA GAGAAACCAC TTGGGTAAAG
    CAAGGCCAAA CTCAAAGGAC ATGCTCTCAA ATCCCACCAG TGTTGAAACG ACATCAAAGG
    GAACTTAGTG CTTTTCAATC AGAACAAGAA GCAACTGACT ATGATGATGC CATCACCATT
    GAAACAATCG AGGATTTTGA CATTTACAGT GAGGACATAA AGCAAGGTCC CCGCAGCTTT
    CAACAGAAAA CAAGGCACTA TTTTATTGCA GCTGTGGAAC GACTCTGGGA CTATGGGATG
    AGTACATCTC ATGTTCTACG AAATAGGTAT CAAAGTGACA ATGTACCTCA GTTCAAGAAA
    GTAGTTTTCC AGGAATTTAC TGATGGCTCC TTTAGTCAGC CCTTATATCG TGGAGAATTA
    AATGAACACC TGGGGTTGTT GGGCCCATAT ATAAGAGCAG AAGTTGAAGA CAACATTATG
    GTAACTTTCA AAAACCAGGC CTCCCGTCCC TACTCCTTCT ATTCTAGCCT CATTTCTTAT
    AAAGAAGATC AGAGAGGAGA AGAACCTAGA AGAAACTTTG TCAAGCCTAA TGAAACCAAA
    ATTTATTTTT GGAAAGTACA ACATCATATG GCACCCACAG AAGATGAGTT TGACTGCAAG
    GCCTGGGCTT ATTTCTCTGA TGTTGATCTT GAAAGAGATA TGCACTCGGG ATTAATTGGA
    CCCCTTCTGA TTTGCCACGC GAACACACTG AATCCTGCTC ATGGGAGACA AGTGTCAGTA
    CAGGAATTTG CTCTGCTTTT CACTATCTTT GATGAGACCA AGAGCTGGTA CTTCACTGAA
    AACGTGAAAA GGAACTGCAA GACACCCTGC AATTTCCAGA TGGAAGACCC CACTTTGAAA
    GAGAATTATC GCTTCCATGC AATCAATGGT TATGTAATGG ATACCCTACC AGGCTTAGTA
    ATGGCTCAAG ATCAAAGGAT TCGATGGTAT CTTCTCAGCA TGGGCAACAA TGAGAACATC
    CAATCTATTC ATTTCAGTGG ACATGTTTTC ACTGTACGGA AAAAAGAGGA GTATAAAATG
    GCAGTGTACA ACCTCTACCC AGGTGTTTTT GAGACTCTGG AAATGATACC ATCCAGAGCT
    GGAATATGGC GAGTAGAATG CCTTATTGGC GAGCACTTAC AGGCTGGGAT GAGCACTCTT
    TTTCTGGTGT ACAGCAAGCA GTGTCAGATT CCTCTTGGAA TGGCTTCTGG AAGCATCCGT
    GATTTCCAGA TTACAGCTTC AGGACATTAT GGACAGTGGG CCCCAAACCT GGCAAGACTT
    CATTATTCCG GATCAATCAA TGCCTGGAGT ACCAAGGAGC CCTTTTCTTG GATCAAGGTA
    GATCTGTTGG CACCAATGAT TGTTCATGGC ATCAAGACTC AGGGTGCTCG TCAGAAATTT
    TCCAGCCTTT ATATCTCTCA ATTTATCATC ATGTATAGCC TGGATGGGAA GAAGTGGCTG
    AGTTATCAAG GAAATTCCAC TGGAACCTTA ATGGTTTTCT TTGGCAATGT GGACTCATCT
    GGGATTAAGC ATAATAGTTT TAATCCTCCA ATTATTGCTC GATATATCCG TTTGCACCCC
    ACTCATTCTA GCATCCGTAG TACTCTTCGC ATGGAGTTGA TGGGCTGTGA TTTAAACAGT
    TGCAGCATAC CATTGGGAAT GGAAAGTAAA GTAATATCAG ATACACAAAT CACTGCCTCA
    TCCTACTTCA CCAACATGTT TGCTACTTGG TCTCCTTCAC AAGCTCGACT TCACCTCCAG
    GGAAGGACTA ATGCCTGGCG ACCTCAGGTG AATGATCCAA AACAATGGTT GCAAGTGGAC
    TTACAAAAGA CAATGAAAGT CACTGGAATA ATAACCCAGG GAGTGAAATC TCTCTTTACC
    AGCATGTTTG TGAAAGAGTT CCTTATTTCC AGCAGTCAAG ATGGCCATCA CTGGACTCAA
    ATTTTATACA ATGGCAAGGT TTTTCAGGGG AATCAGGACT CATCCACACC TATGATGAAT
    TCTCTAGACC CACCATTACT CACTCGCTAT CTTCGAATTC ACCCCCAGAT CTGGGAGCAC
    CAAATTGCTC TGAGGCTTGA GATTCTAGGA TGTGAGGCCC AGCAGCAATA CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006527852.1
    CDS1073..8026
    Translation

    Target ORF information:

    RefSeq Version XM_006527789.3
    Organism Mus musculus(house mouse)
    Definition Mus musculus coagulation factor VIII (F8), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006527789.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    ATGCAAATAG CACTCTTCGC TTGCTTCTTT CTGAGCCTTT TCAATTTCTG CTCTAGTGCC 
    ATCAGAAGAT ACTACCTTGG TGCAGTGGAA TTGTCCTGGA ACTATATTCA GAGTGATCTG
    CTCAGTGTGC TGCATACAGA CTCAAGATTT CTTCCTAGAA TGTCAACATC TTTTCCATTC
    AACACCTCCA TCATGTATAA AAAGACTGTG TTTGTAGAGT ACAAGGACCA GCTTTTCAAC
    ATTGCCAAGC CCAGGCCACC CTGGATGGGT TTGCTAGGTC CTACCATTTG GACTGAGGTT
    CATGACACAG TGGTCATTAC ACTTAAAAAC ATGGCTTCTC ATCCTGTCAG TCTTCATGCT
    GTTGGTGTGT CCTACTGGAA AGCTTCTGAG GGAGATGAAT ATGAAGATCA GACAAGCCAA
    ATGGAGAAGG AAGATGATAA AGTTTTCCCT GGTGAAAGTC ATACTTATGT TTGGCAAGTC
    CTGAAAGAGA ATGGTCCAAT GGCCTCTGAC CCTCCATGTC TCACTTACTC ATATATGTCT
    CATGTGGATC TGGTGAAAGA TTTGAATTCA GGCCTCATTG GAGCTCTGCT AGTATGTAAA
    GAAGGCAGTC TCTCCAAAGA AAGAACACAG ATGTTGTACC AATTTGTACT GCTTTTTGCT
    GTATTTGATG AAGGGAAGAG CTGGCACTCA GAAACAAACG ACTCTTATAC ACAGTCTATG
    GATTCTGCAT CTGCTAGAGA CTGGCCTAAA ATGCACACAG TCAATGGCTA TGTAAACAGG
    TCTCTTCCAG GTCTGATTGG ATGCCATAGG AAATCAGTCT ACTGGCACGT GATTGGAATG
    GGCACCACTC CTGAAATACA CTCAATATTC CTCGAAGGTC ACACATTTTT TGTGAGGAAC
    CACCGTCAAG CTTCATTGGA GATATCACCA ATAACTTTCC TTACTGCTCA AACACTCTTG
    ATAGATCTTG GGCAGTTCCT ACTATTTTGT CATATCTCTT CCCATAAACA TGATGGCATG
    GAAGCTTATG TCAAAGTAGA TAGCTGCCCT GAGGAATCCC AATGGCAAAA GAAAAATAAT
    AATGAGGAAA TGGAAGATTA TGATGATGAT CTTTATTCAG AAATGGATAT GTTCACATTG
    GATTATGACA GCTCTCCTTT TATCCAAATT CGCTCGGTTG CTAAAAAGTA CCCTAAAACT
    TGGATACATT ATATTTCTGC TGAGGAGGAA GACTGGGACT ATGCACCTTC AGTTCCTACC
    TCGGATAATG GAAGTTATAA AAGCCAGTAT CTGAGCAATG GTCCTCATCG GATTGGTAGG
    AAATATAAAA AAGTCAGATT TATAGCATAC ACAGATGAAA CCTTTAAGAC TCGTGAAACT
    ATTCAGCATG AATCAGGACT CTTGGGACCT TTACTTTATG GAGAAGTTGG AGACACACTG
    TTGATTATTT TTAAGAATCA AGCAAGCCGA CCATATAACA TTTACCCTCA TGGAATCACT
    GATGTCAGTC CTCTACATGC AAGGAGATTG CCAAGAGGTA TAAAGCACGT GAAGGATTTG
    CCAATTCATC CAGGAGAGAT ATTCAAGTAC AAGTGGACAG TTACAGTAGA AGATGGACCA
    ACTAAATCAG ATCCACGGTG CCTGACCCGC TATTATTCAA GTTTCATTAA CCCTGAGAGA
    GATCTAGCTT CAGGACTGAT TGGCCCTCTT CTCATCTGCT ACAAAGAATC TGTAGATCAA
    AGGGGAAACC AGATGATGTC AGACAAAAGA AATGTCATCC TGTTTTCTAT ATTTGATGAG
    AACCAAAGCT GGTACATCAC AGAGAACATG CAACGCTTCC TCCCCAATGC AGCTAAAACA
    CAGCCCCAGG ACCCTGGGTT CCAGGCCTCC AACATCATGC ACAGCATCAA TGGCTATGTT
    TTTGATAGCT TGGAGTTGAC AGTTTGTTTG CATGAGGTGG CATACTGGCA CATTCTCAGT
    GTTGGAGCAC AGACAGACTT CTTATCTATC TTCTTCTCTG GATATACTTT CAAACACAAA
    ATGGTCTATG AAGATACACT TACCCTGTTC CCATTCTCAG GAGAAACTGT CTTTATGTCG
    ATGGAAAACC CAGGTCTATG GGTCTTGGGG TGTCATAATT CAGACTTTCG GAAGAGAGGT
    ATGACAGCAT TGCTGAAAGT TTCTAGTTGT GACAAGAGCA CTAGTGATTA TTATGAAGAA
    ATATATGAAG ATATTCCAAC ACAGTTGGTG AATGAGAACA ATGTCATTGA TCCCAGAAGC
    TTCTTCCAGA ATACAAATCA TCCTAATACT AGGAAAAAGA AATTCAAAGA TTCCACAATT
    CCAAAAAATG ATATGGAGAA GATTGAGCCT CAGTTTGAAG AGATAGCAGA GATGCTTAAA
    GTACAGAGTG TCTCAGTTAG TGACATGTTG ATGCTCTTGG GACAGAGTCA TCCTACTCCA
    CATGGCTTAT TTTTATCAGA TGGCCAAGAA GCCATCTATG AGGCTATTCA TGATGATCAT
    TCACCAAATG CAATAGACAG CAATGAAGGC CCATCTAAAG TGACCCAACT CAGGCCAGAA
    TCCCATCACA GTGAGAAAAT AGTATTTACT CCTCAGCCCG GCCTCCAGTT AAGATCCAAT
    AAAAGTTTGG AGACAACTAT AGAAGTAAAG TGGAAGAAAC TTGGTTTGCA AGTTTCTAGT
    TTGCCAAGTA ATCTAATGAC TACAACAATT CTGTCAGACA ATTTGAAAGC AACTTTTGAA
    AAGACAGATT CTTCAGGATT TCCAGATATG CCAGTTCACT CTAGTAGTAA ATTAAGTACT
    ACTGCATTTG GTAAGAAAGC ATATTCCCTT GTTGGGTCTC ATGTACCTTT AAACGTGAGT
    GAAGAAAATA GTGATTCCAA CATATTGGAT TCAACTTTAA TGTATAGTCA AGAAAGTTTA
    CCAAGAGATA ATATATTATC AATGGAGAAT GATAGATTAC TCAGAGAGAA GAGGTTTCAT
    GGAATTGCTT TATTGACCAA AGATAATACT TTATTCAAAG ACAATGTCTC CTTAATGAAA
    ACAAACAAAA CATATAATCA TTCAACAACT AATGAAAAAC TACACACTGA GAGCCCAACA
    TCAATTGAGA ATAGTACAAC AGACTTGCAA GATGCCATAT TAAAGGTCAA TAGTGAGATT
    CAAGAAGTAA CAGCTTTGAT TCATGATGGA ACACTTTTAG GCAAAAATTC TACATATTTG
    AGACTAAACC ATATGCTAAA TAGAACTACC TCAACAAAAA ATAAAGACAT ATTTCATAGA
    AAAGATGAAG ATCCTATTCC ACAAGATGAA GAGAATACAA TCATGCCATT TTCCAAGATG
    TTGTTCTTGT CAGAATCTTC AAATTGGTTT AAAAAGACCA ATGGAAATAA TTCCTTGAAC
    TCTGAGCAAG AACATAGTCC AAAGCAATTA GTATATTTAA TGTTTAAAAA ATATGTAAAA
    AATCAAAGTT TCTTGTCAGA GAAAAATAAA GTCACAGTAG AACAGGATGG ATTTACAAAG
    AACATAGGAC TTAAAGACAT GGCTTTTCCA CATAATATGA GCATATTTCT TACCACTTTG
    TCTAACGTAC ATGAAAATGG TAGGCACAAT CAAGAAAAAA ATATTCAGGA AGAGATAGAG
    AAGGAAGCAC TAATTGAAGA GAAAGTAGTT TTGCCCCAGG TGCACGAAGC AACTGGCTCT
    AAGAATTTCT TGAAAGACAT ATTGATACTA GGCACTAGGC AAAATATAAG TTTATATGAA
    GTACATGTAC CAGTACTTCA AAACATCACA TCAATAAACA ATTCAACAAA TACAGTACAG
    ATTCACATGG AGCATTTCTT TAAAAGAAGG AAGGACAAGG AAACAAATTC AGAAGGCTTG
    GTAAATAAAA CCAGAGAAAT GGTAAAAAAC TATCCAAGCC AGAAGAATAT TACTACTCAA
    CGTAGTAAAC GGGCTTTGGG ACAATTCAGA CTGTCAACTC AATGGCTTAA AACCATAAAC
    TGTTCAACAC AGTGTATCAT TAAACAGATA GACCACAGCA AGGAAATGAA AAAGTTCATT
    ACTAAATCTT CCTTATCAGA TTCTTCTGTG ATTAAAAGCA CCACTCAGAC AAATAGTTCT
    GACTCACACA TTGTAAAAAC ATCAGCATTT CCACCAATAG ATCTCAAAAG GAGTCCATTC
    CAAAACAAAT TTTCTCATGT TCAAGCATCA TCCTACATTT ATGACTTTAA GACAAAAAGT
    TCAAGAATTC AAGAAAGCAA TAATTTCTTA AAAGAAACCA AAATAAATAA CCCTTCTTTA
    GCCATTCTAC CATGGAATAT GTTCATAGAT CAAGGAAAAT TTACCTCCCC AGGGAAAAGT
    AACACAAACT CAGTCACATA TAAGAAACGT GAGAACATTA TTTTCTTGAA ACCAACTTTG
    CCTGAAGAAT CTGGCAAAAT TGAATTGCTT CCTCAAGTTT CCATTCAAGA GGAAGAAATT
    TTACCTACAG AAACTAGCCA TGGATCTCCT GGACACTTGA ATCTCATGAA AGAGGTCTTT
    CTTCAGAAAA TACAGGGGCC TACTAAATGG AATAAAGCAA AGAGGCATGG AGAAAGTATA
    AAAGGTAAAA CAGAGAGCTC TAAAAATACT CGCTCAAAAC TGCTAAATCA TCATGCTTGG
    GATTATCATT ATGCTGCACA GATACCAAAA GATATGTGGA AATCCAAAGA GAAGTCACCA
    GAAATTATAT CCATTAAGCA AGAGGACACC ATTTTGTCTC TGAGGCCTCA TGGAAACAGT
    CATTCAATAG GGGCAAATGA GAAACAAAAT TGGCCTCAAA GAGAAACCAC TTGGGTAAAG
    CAAGGCCAAA CTCAAAGGAC ATGCTCTCAA ATCCCACCAG TGTTGAAACG ACATCAAAGG
    GAACTTAGTG CTTTTCAATC AGAACAAGAA GCAACTGACT ATGATGATGC CATCACCATT
    GAAACAATCG AGGATTTTGA CATTTACAGT GAGGACATAA AGCAAGGTCC CCGCAGCTTT
    CAACAGAAAA CAAGGCACTA TTTTATTGCA GCTGTGGAAC GACTCTGGGA CTATGGGATG
    AGTACATCTC ATGTTCTACG AAATAGGTAT CAAAGTGACA ATGTACCTCA GTTCAAGAAA
    GTAGTTTTCC AGGAATTTAC TGATGGCTCC TTTAGTCAGC CCTTATATCG TGGAGAATTA
    AATGAACACC TGGGGTTGTT GGGCCCATAT ATAAGAGCAG AAGTTGAAGA CAACATTATG
    GTAACTTTCA AAAACCAGGC CTCCCGTCCC TACTCCTTCT ATTCTAGCCT CATTTCTTAT
    AAAGAAGATC AGAGAGGAGA AGAACCTAGA AGAAACTTTG TCAAGCCTAA TGAAACCAAA
    ATTTATTTTT GGAAAGTACA ACATCATATG GCACCCACAG AAGATGAGTT TGACTGCAAG
    GCCTGGGCTT ATTTCTCTGA TGTTGATCTT GAAAGAGATA TGCACTCGGG ATTAATTGGA
    CCCCTTCTGA TTTGCCACGC GAACACACTG AATCCTGCTC ATGGGAGACA AGTGTCAGTA
    CAGGAATTTG CTCTGCTTTT CACTATCTTT GATGAGACCA AGAGCTGGTA CTTCACTGAA
    AACGTGAAAA GGAACTGCAA GACACCCTGC AATTTCCAGA TGGAAGACCC CACTTTGAAA
    GAGAATTATC GCTTCCATGC AATCAATGGT TATGTAATGG ATACCCTACC AGGCTTAGTA
    ATGGCTCAAG ATCAAAGGAT TCGATGGTAT CTTCTCAGCA TGGGCAACAA TGAGAACATC
    CAATCTATTC ATTTCAGTGG ACATGTTTTC ACTGTACGGA AAAAAGAGGA GTATAAAATG
    GCAGTGTACA ACCTCTACCC AGGTGTTTTT GAGACTCTGG AAATGATACC ATCCAGAGCT
    GGAATATGGC GAGTAGAATG CCTTATTGGC GAGCACTTAC AGGCTGGGAT GAGCACTCTT
    TTTCTGGTGT ACAGCAAGCA GTGTCAGATT CCTCTTGGAA TGGCTTCTGG AAGCATCCGT
    GATTTCCAGA TTACAGCTTC AGGACATTAT GGACAGTGGG CCCCAAACCT GGCAAGACTT
    CATTATTCCG GATCAATCAA TGCCTGGAGT ACCAAGGAGC CCTTTTCTTG GATCAAGGTA
    GATCTGTTGG CACCAATGAT TGTTCATGGC ATCAAGACTC AGGGTGCTCG TCAGAAATTT
    TCCAGCCTTT ATATCTCTCA ATTTATCATC ATGTATAGCC TGGATGGGAA GAAGTGGCTG
    AGTTATCAAG GAAATTCCAC TGGAACCTTA ATGGTTTTCT TTGGCAATGT GGACTCATCT
    GGGATTAAGC ATAATAGTTT TAATCCTCCA ATTATTGCTC GATATATCCG TTTGCACCCC
    ACTCATTCTA GCATCCGTAG TACTCTTCGC ATGGAGTTGA TGGGCTGTGA TTTAAACAGT
    TGCAGCATAC CATTGGGAAT GGAAAGTAAA GTAATATCAG ATACACAAAT CACTGCCTCA
    TCCTACTTCA CCAACATGTT TGCTACTTGG TCTCCTTCAC AAGCTCGACT TCACCTCCAG
    GGAAGGACTA ATGCCTGGCG ACCTCAGGTG AATGATCCAA AACAATGGTT GCAAGTGGAC
    TTACAAAAGA CAATGAAAGT CACTGGAATA ATAACCCAGG GAGTGAAATC TCTCTTTACC
    AGCATGTTTG TGAAAGAGTT CCTTATTTCC AGCAGTCAAG ATGGCCATCA CTGGACTCAA
    ATTTTATACA ATGGCAAGGT TTTTCAGGGG AATCAGGACT CATCCACACC TATGATGAAT
    TCTCTAGACC CACCATTACT CACTCGCTAT CTTCGAATTC ACCCCCAGAT CTGGGAGCAC
    CAAATTGCTC TGAGGCTTGA GATTCTAGGA TGTGAGGCCC AGCAGCAATA CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu56129
    Clone ID Related Accession (Same CDS sequence) XM_006527790.4 , XM_006527790.3
    Accession Version XM_006527790.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6939bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-21
    Organism Mus musculus(house mouse)
    Product coagulation factor VIII isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000086.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006527790.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    ATGCAACCAG CAGTACTTCA CTCTAAGCGA CAAAGTGCCA TCAGAAGATA CTACCTTGGT 
    GCAGTGGAAT TGTCCTGGAA CTATATTCAG AGTGATCTGC TCAGTGTGCT GCATACAGAC
    TCAAGATTTC TTCCTAGAAT GTCAACATCT TTTCCATTCA ACACCTCCAT CATGTATAAA
    AAGACTGTGT TTGTAGAGTA CAAGGACCAG CTTTTCAACA TTGCCAAGCC CAGGCCACCC
    TGGATGGGTT TGCTAGGTCC TACCATTTGG ACTGAGGTTC ATGACACAGT GGTCATTACA
    CTTAAAAACA TGGCTTCTCA TCCTGTCAGT CTTCATGCTG TTGGTGTGTC CTACTGGAAA
    GCTTCTGAGG GAGATGAATA TGAAGATCAG ACAAGCCAAA TGGAGAAGGA AGATGATAAA
    GTTTTCCCTG GTGAAAGTCA TACTTATGTT TGGCAAGTCC TGAAAGAGAA TGGTCCAATG
    GCCTCTGACC CTCCATGTCT CACTTACTCA TATATGTCTC ATGTGGATCT GGTGAAAGAT
    TTGAATTCAG GCCTCATTGG AGCTCTGCTA GTATGTAAAG AAGGCAGTCT CTCCAAAGAA
    AGAACACAGA TGTTGTACCA ATTTGTACTG CTTTTTGCTG TATTTGATGA AGGGAAGAGC
    TGGCACTCAG AAACAAACGA CTCTTATACA CAGTCTATGG ATTCTGCATC TGCTAGAGAC
    TGGCCTAAAA TGCACACAGT CAATGGCTAT GTAAACAGGT CTCTTCCAGG TCTGATTGGA
    TGCCATAGGA AATCAGTCTA CTGGCACGTG ATTGGAATGG GCACCACTCC TGAAATACAC
    TCAATATTCC TCGAAGGTCA CACATTTTTT GTGAGGAACC ACCGTCAAGC TTCATTGGAG
    ATATCACCAA TAACTTTCCT TACTGCTCAA ACACTCTTGA TAGATCTTGG GCAGTTCCTA
    CTATTTTGTC ATATCTCTTC CCATAAACAT GATGGCATGG AAGCTTATGT CAAAGTAGAT
    AGCTGCCCTG AGGAATCCCA ATGGCAAAAG AAAAATAATA ATGAGGAAAT GGAAGATTAT
    GATGATGATC TTTATTCAGA AATGGATATG TTCACATTGG ATTATGACAG CTCTCCTTTT
    ATCCAAATTC GCTCGGTTGC TAAAAAGTAC CCTAAAACTT GGATACATTA TATTTCTGCT
    GAGGAGGAAG ACTGGGACTA TGCACCTTCA GTTCCTACCT CGGATAATGG AAGTTATAAA
    AGCCAGTATC TGAGCAATGG TCCTCATCGG ATTGGTAGGA AATATAAAAA AGTCAGATTT
    ATAGCATACA CAGATGAAAC CTTTAAGACT CGTGAAACTA TTCAGCATGA ATCAGGACTC
    TTGGGACCTT TACTTTATGG AGAAGTTGGA GACACACTGT TGATTATTTT TAAGAATCAA
    GCAAGCCGAC CATATAACAT TTACCCTCAT GGAATCACTG ATGTCAGTCC TCTACATGCA
    AGGAGATTGC CAAGAGGTAT AAAGCACGTG AAGGATTTGC CAATTCATCC AGGAGAGATA
    TTCAAGTACA AGTGGACAGT TACAGTAGAA GATGGACCAA CTAAATCAGA TCCACGGTGC
    CTGACCCGCT ATTATTCAAG TTTCATTAAC CCTGAGAGAG ATCTAGCTTC AGGACTGATT
    GGCCCTCTTC TCATCTGCTA CAAAGAATCT GTAGATCAAA GGGGAAACCA GATGATGTCA
    GACAAAAGAA ATGTCATCCT GTTTTCTATA TTTGATGAGA ACCAAAGCTG GTACATCACA
    GAGAACATGC AACGCTTCCT CCCCAATGCA GCTAAAACAC AGCCCCAGGA CCCTGGGTTC
    CAGGCCTCCA ACATCATGCA CAGCATCAAT GGCTATGTTT TTGATAGCTT GGAGTTGACA
    GTTTGTTTGC ATGAGGTGGC ATACTGGCAC ATTCTCAGTG TTGGAGCACA GACAGACTTC
    TTATCTATCT TCTTCTCTGG ATATACTTTC AAACACAAAA TGGTCTATGA AGATACACTT
    ACCCTGTTCC CATTCTCAGG AGAAACTGTC TTTATGTCGA TGGAAAACCC AGGTCTATGG
    GTCTTGGGGT GTCATAATTC AGACTTTCGG AAGAGAGGTA TGACAGCATT GCTGAAAGTT
    TCTAGTTGTG ACAAGAGCAC TAGTGATTAT TATGAAGAAA TATATGAAGA TATTCCAACA
    CAGTTGGTGA ATGAGAACAA TGTCATTGAT CCCAGAAGCT TCTTCCAGAA TACAAATCAT
    CCTAATACTA GGAAAAAGAA ATTCAAAGAT TCCACAATTC CAAAAAATGA TATGGAGAAG
    ATTGAGCCTC AGTTTGAAGA GATAGCAGAG ATGCTTAAAG TACAGAGTGT CTCAGTTAGT
    GACATGTTGA TGCTCTTGGG ACAGAGTCAT CCTACTCCAC ATGGCTTATT TTTATCAGAT
    GGCCAAGAAG CCATCTATGA GGCTATTCAT GATGATCATT CACCAAATGC AATAGACAGC
    AATGAAGGCC CATCTAAAGT GACCCAACTC AGGCCAGAAT CCCATCACAG TGAGAAAATA
    GTATTTACTC CTCAGCCCGG CCTCCAGTTA AGATCCAATA AAAGTTTGGA GACAACTATA
    GAAGTAAAGT GGAAGAAACT TGGTTTGCAA GTTTCTAGTT TGCCAAGTAA TCTAATGACT
    ACAACAATTC TGTCAGACAA TTTGAAAGCA ACTTTTGAAA AGACAGATTC TTCAGGATTT
    CCAGATATGC CAGTTCACTC TAGTAGTAAA TTAAGTACTA CTGCATTTGG TAAGAAAGCA
    TATTCCCTTG TTGGGTCTCA TGTACCTTTA AACGTGAGTG AAGAAAATAG TGATTCCAAC
    ATATTGGATT CAACTTTAAT GTATAGTCAA GAAAGTTTAC CAAGAGATAA TATATTATCA
    ATGGAGAATG ATAGATTACT CAGAGAGAAG AGGTTTCATG GAATTGCTTT ATTGACCAAA
    GATAATACTT TATTCAAAGA CAATGTCTCC TTAATGAAAA CAAACAAAAC ATATAATCAT
    TCAACAACTA ATGAAAAACT ACACACTGAG AGCCCAACAT CAATTGAGAA TAGTACAACA
    GACTTGCAAG ATGCCATATT AAAGGTCAAT AGTGAGATTC AAGAAGTAAC AGCTTTGATT
    CATGATGGAA CACTTTTAGG CAAAAATTCT ACATATTTGA GACTAAACCA TATGCTAAAT
    AGAACTACCT CAACAAAAAA TAAAGACATA TTTCATAGAA AAGATGAAGA TCCTATTCCA
    CAAGATGAAG AGAATACAAT CATGCCATTT TCCAAGATGT TGTTCTTGTC AGAATCTTCA
    AATTGGTTTA AAAAGACCAA TGGAAATAAT TCCTTGAACT CTGAGCAAGA ACATAGTCCA
    AAGCAATTAG TATATTTAAT GTTTAAAAAA TATGTAAAAA ATCAAAGTTT CTTGTCAGAG
    AAAAATAAAG TCACAGTAGA ACAGGATGGA TTTACAAAGA ACATAGGACT TAAAGACATG
    GCTTTTCCAC ATAATATGAG CATATTTCTT ACCACTTTGT CTAACGTACA TGAAAATGGT
    AGGCACAATC AAGAAAAAAA TATTCAGGAA GAGATAGAGA AGGAAGCACT AATTGAAGAG
    AAAGTAGTTT TGCCCCAGGT GCACGAAGCA ACTGGCTCTA AGAATTTCTT GAAAGACATA
    TTGATACTAG GCACTAGGCA AAATATAAGT TTATATGAAG TACATGTACC AGTACTTCAA
    AACATCACAT CAATAAACAA TTCAACAAAT ACAGTACAGA TTCACATGGA GCATTTCTTT
    AAAAGAAGGA AGGACAAGGA AACAAATTCA GAAGGCTTGG TAAATAAAAC CAGAGAAATG
    GTAAAAAACT ATCCAAGCCA GAAGAATATT ACTACTCAAC GTAGTAAACG GGCTTTGGGA
    CAATTCAGAC TGTCAACTCA ATGGCTTAAA ACCATAAACT GTTCAACACA GTGTATCATT
    AAACAGATAG ACCACAGCAA GGAAATGAAA AAGTTCATTA CTAAATCTTC CTTATCAGAT
    TCTTCTGTGA TTAAAAGCAC CACTCAGACA AATAGTTCTG ACTCACACAT TGTAAAAACA
    TCAGCATTTC CACCAATAGA TCTCAAAAGG AGTCCATTCC AAAACAAATT TTCTCATGTT
    CAAGCATCAT CCTACATTTA TGACTTTAAG ACAAAAAGTT CAAGAATTCA AGAAAGCAAT
    AATTTCTTAA AAGAAACCAA AATAAATAAC CCTTCTTTAG CCATTCTACC ATGGAATATG
    TTCATAGATC AAGGAAAATT TACCTCCCCA GGGAAAAGTA ACACAAACTC AGTCACATAT
    AAGAAACGTG AGAACATTAT TTTCTTGAAA CCAACTTTGC CTGAAGAATC TGGCAAAATT
    GAATTGCTTC CTCAAGTTTC CATTCAAGAG GAAGAAATTT TACCTACAGA AACTAGCCAT
    GGATCTCCTG GACACTTGAA TCTCATGAAA GAGGTCTTTC TTCAGAAAAT ACAGGGGCCT
    ACTAAATGGA ATAAAGCAAA GAGGCATGGA GAAAGTATAA AAGGTAAAAC AGAGAGCTCT
    AAAAATACTC GCTCAAAACT GCTAAATCAT CATGCTTGGG ATTATCATTA TGCTGCACAG
    ATACCAAAAG ATATGTGGAA ATCCAAAGAG AAGTCACCAG AAATTATATC CATTAAGCAA
    GAGGACACCA TTTTGTCTCT GAGGCCTCAT GGAAACAGTC ATTCAATAGG GGCAAATGAG
    AAACAAAATT GGCCTCAAAG AGAAACCACT TGGGTAAAGC AAGGCCAAAC TCAAAGGACA
    TGCTCTCAAA TCCCACCAGT GTTGAAACGA CATCAAAGGG AACTTAGTGC TTTTCAATCA
    GAACAAGAAG CAACTGACTA TGATGATGCC ATCACCATTG AAACAATCGA GGATTTTGAC
    ATTTACAGTG AGGACATAAA GCAAGGTCCC CGCAGCTTTC AACAGAAAAC AAGGCACTAT
    TTTATTGCAG CTGTGGAACG ACTCTGGGAC TATGGGATGA GTACATCTCA TGTTCTACGA
    AATAGGTATC AAAGTGACAA TGTACCTCAG TTCAAGAAAG TAGTTTTCCA GGAATTTACT
    GATGGCTCCT TTAGTCAGCC CTTATATCGT GGAGAATTAA ATGAACACCT GGGGTTGTTG
    GGCCCATATA TAAGAGCAGA AGTTGAAGAC AACATTATGG TAACTTTCAA AAACCAGGCC
    TCCCGTCCCT ACTCCTTCTA TTCTAGCCTC ATTTCTTATA AAGAAGATCA GAGAGGAGAA
    GAACCTAGAA GAAACTTTGT CAAGCCTAAT GAAACCAAAA TTTATTTTTG GAAAGTACAA
    CATCATATGG CACCCACAGA AGATGAGTTT GACTGCAAGG CCTGGGCTTA TTTCTCTGAT
    GTTGATCTTG AAAGAGATAT GCACTCGGGA TTAATTGGAC CCCTTCTGAT TTGCCACGCG
    AACACACTGA ATCCTGCTCA TGGGAGACAA GTGTCAGTAC AGGAATTTGC TCTGCTTTTC
    ACTATCTTTG ATGAGACCAA GAGCTGGTAC TTCACTGAAA ACGTGAAAAG GAACTGCAAG
    ACACCCTGCA ATTTCCAGAT GGAAGACCCC ACTTTGAAAG AGAATTATCG CTTCCATGCA
    ATCAATGGTT ATGTAATGGA TACCCTACCA GGCTTAGTAA TGGCTCAAGA TCAAAGGATT
    CGATGGTATC TTCTCAGCAT GGGCAACAAT GAGAACATCC AATCTATTCA TTTCAGTGGA
    CATGTTTTCA CTGTACGGAA AAAAGAGGAG TATAAAATGG CAGTGTACAA CCTCTACCCA
    GGTGTTTTTG AGACTCTGGA AATGATACCA TCCAGAGCTG GAATATGGCG AGTAGAATGC
    CTTATTGGCG AGCACTTACA GGCTGGGATG AGCACTCTTT TTCTGGTGTA CAGCAAGCAG
    TGTCAGATTC CTCTTGGAAT GGCTTCTGGA AGCATCCGTG ATTTCCAGAT TACAGCTTCA
    GGACATTATG GACAGTGGGC CCCAAACCTG GCAAGACTTC ATTATTCCGG ATCAATCAAT
    GCCTGGAGTA CCAAGGAGCC CTTTTCTTGG ATCAAGGTAG ATCTGTTGGC ACCAATGATT
    GTTCATGGCA TCAAGACTCA GGGTGCTCGT CAGAAATTTT CCAGCCTTTA TATCTCTCAA
    TTTATCATCA TGTATAGCCT GGATGGGAAG AAGTGGCTGA GTTATCAAGG AAATTCCACT
    GGAACCTTAA TGGTTTTCTT TGGCAATGTG GACTCATCTG GGATTAAGCA TAATAGTTTT
    AATCCTCCAA TTATTGCTCG ATATATCCGT TTGCACCCCA CTCATTCTAG CATCCGTAGT
    ACTCTTCGCA TGGAGTTGAT GGGCTGTGAT TTAAACAGTT GCAGCATACC ATTGGGAATG
    GAAAGTAAAG TAATATCAGA TACACAAATC ACTGCCTCAT CCTACTTCAC CAACATGTTT
    GCTACTTGGT CTCCTTCACA AGCTCGACTT CACCTCCAGG GAAGGACTAA TGCCTGGCGA
    CCTCAGGTGA ATGATCCAAA ACAATGGTTG CAAGTGGACT TACAAAAGAC AATGAAAGTC
    ACTGGAATAA TAACCCAGGG AGTGAAATCT CTCTTTACCA GCATGTTTGT GAAAGAGTTC
    CTTATTTCCA GCAGTCAAGA TGGCCATCAC TGGACTCAAA TTTTATACAA TGGCAAGGTA
    AAGGTTTTTC AGGGGAATCA GGACTCATCC ACACCTATGA TGAATTCTCT AGACCCACCA
    TTACTCACTC GCTATCTTCG AATTCACCCC CAGATCTGGG AGCACCAAAT TGCTCTGAGG
    CTTGAGATTC TAGGATGTGA GGCCCAGCAG CAATACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006527853.1
    CDS997..7935
    Translation

    Target ORF information:

    RefSeq Version XM_006527790.3
    Organism Mus musculus(house mouse)
    Definition Mus musculus coagulation factor VIII (F8), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006527790.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    ATGCAACCAG CAGTACTTCA CTCTAAGCGA CAAAGTGCCA TCAGAAGATA CTACCTTGGT 
    GCAGTGGAAT TGTCCTGGAA CTATATTCAG AGTGATCTGC TCAGTGTGCT GCATACAGAC
    TCAAGATTTC TTCCTAGAAT GTCAACATCT TTTCCATTCA ACACCTCCAT CATGTATAAA
    AAGACTGTGT TTGTAGAGTA CAAGGACCAG CTTTTCAACA TTGCCAAGCC CAGGCCACCC
    TGGATGGGTT TGCTAGGTCC TACCATTTGG ACTGAGGTTC ATGACACAGT GGTCATTACA
    CTTAAAAACA TGGCTTCTCA TCCTGTCAGT CTTCATGCTG TTGGTGTGTC CTACTGGAAA
    GCTTCTGAGG GAGATGAATA TGAAGATCAG ACAAGCCAAA TGGAGAAGGA AGATGATAAA
    GTTTTCCCTG GTGAAAGTCA TACTTATGTT TGGCAAGTCC TGAAAGAGAA TGGTCCAATG
    GCCTCTGACC CTCCATGTCT CACTTACTCA TATATGTCTC ATGTGGATCT GGTGAAAGAT
    TTGAATTCAG GCCTCATTGG AGCTCTGCTA GTATGTAAAG AAGGCAGTCT CTCCAAAGAA
    AGAACACAGA TGTTGTACCA ATTTGTACTG CTTTTTGCTG TATTTGATGA AGGGAAGAGC
    TGGCACTCAG AAACAAACGA CTCTTATACA CAGTCTATGG ATTCTGCATC TGCTAGAGAC
    TGGCCTAAAA TGCACACAGT CAATGGCTAT GTAAACAGGT CTCTTCCAGG TCTGATTGGA
    TGCCATAGGA AATCAGTCTA CTGGCACGTG ATTGGAATGG GCACCACTCC TGAAATACAC
    TCAATATTCC TCGAAGGTCA CACATTTTTT GTGAGGAACC ACCGTCAAGC TTCATTGGAG
    ATATCACCAA TAACTTTCCT TACTGCTCAA ACACTCTTGA TAGATCTTGG GCAGTTCCTA
    CTATTTTGTC ATATCTCTTC CCATAAACAT GATGGCATGG AAGCTTATGT CAAAGTAGAT
    AGCTGCCCTG AGGAATCCCA ATGGCAAAAG AAAAATAATA ATGAGGAAAT GGAAGATTAT
    GATGATGATC TTTATTCAGA AATGGATATG TTCACATTGG ATTATGACAG CTCTCCTTTT
    ATCCAAATTC GCTCGGTTGC TAAAAAGTAC CCTAAAACTT GGATACATTA TATTTCTGCT
    GAGGAGGAAG ACTGGGACTA TGCACCTTCA GTTCCTACCT CGGATAATGG AAGTTATAAA
    AGCCAGTATC TGAGCAATGG TCCTCATCGG ATTGGTAGGA AATATAAAAA AGTCAGATTT
    ATAGCATACA CAGATGAAAC CTTTAAGACT CGTGAAACTA TTCAGCATGA ATCAGGACTC
    TTGGGACCTT TACTTTATGG AGAAGTTGGA GACACACTGT TGATTATTTT TAAGAATCAA
    GCAAGCCGAC CATATAACAT TTACCCTCAT GGAATCACTG ATGTCAGTCC TCTACATGCA
    AGGAGATTGC CAAGAGGTAT AAAGCACGTG AAGGATTTGC CAATTCATCC AGGAGAGATA
    TTCAAGTACA AGTGGACAGT TACAGTAGAA GATGGACCAA CTAAATCAGA TCCACGGTGC
    CTGACCCGCT ATTATTCAAG TTTCATTAAC CCTGAGAGAG ATCTAGCTTC AGGACTGATT
    GGCCCTCTTC TCATCTGCTA CAAAGAATCT GTAGATCAAA GGGGAAACCA GATGATGTCA
    GACAAAAGAA ATGTCATCCT GTTTTCTATA TTTGATGAGA ACCAAAGCTG GTACATCACA
    GAGAACATGC AACGCTTCCT CCCCAATGCA GCTAAAACAC AGCCCCAGGA CCCTGGGTTC
    CAGGCCTCCA ACATCATGCA CAGCATCAAT GGCTATGTTT TTGATAGCTT GGAGTTGACA
    GTTTGTTTGC ATGAGGTGGC ATACTGGCAC ATTCTCAGTG TTGGAGCACA GACAGACTTC
    TTATCTATCT TCTTCTCTGG ATATACTTTC AAACACAAAA TGGTCTATGA AGATACACTT
    ACCCTGTTCC CATTCTCAGG AGAAACTGTC TTTATGTCGA TGGAAAACCC AGGTCTATGG
    GTCTTGGGGT GTCATAATTC AGACTTTCGG AAGAGAGGTA TGACAGCATT GCTGAAAGTT
    TCTAGTTGTG ACAAGAGCAC TAGTGATTAT TATGAAGAAA TATATGAAGA TATTCCAACA
    CAGTTGGTGA ATGAGAACAA TGTCATTGAT CCCAGAAGCT TCTTCCAGAA TACAAATCAT
    CCTAATACTA GGAAAAAGAA ATTCAAAGAT TCCACAATTC CAAAAAATGA TATGGAGAAG
    ATTGAGCCTC AGTTTGAAGA GATAGCAGAG ATGCTTAAAG TACAGAGTGT CTCAGTTAGT
    GACATGTTGA TGCTCTTGGG ACAGAGTCAT CCTACTCCAC ATGGCTTATT TTTATCAGAT
    GGCCAAGAAG CCATCTATGA GGCTATTCAT GATGATCATT CACCAAATGC AATAGACAGC
    AATGAAGGCC CATCTAAAGT GACCCAACTC AGGCCAGAAT CCCATCACAG TGAGAAAATA
    GTATTTACTC CTCAGCCCGG CCTCCAGTTA AGATCCAATA AAAGTTTGGA GACAACTATA
    GAAGTAAAGT GGAAGAAACT TGGTTTGCAA GTTTCTAGTT TGCCAAGTAA TCTAATGACT
    ACAACAATTC TGTCAGACAA TTTGAAAGCA ACTTTTGAAA AGACAGATTC TTCAGGATTT
    CCAGATATGC CAGTTCACTC TAGTAGTAAA TTAAGTACTA CTGCATTTGG TAAGAAAGCA
    TATTCCCTTG TTGGGTCTCA TGTACCTTTA AACGTGAGTG AAGAAAATAG TGATTCCAAC
    ATATTGGATT CAACTTTAAT GTATAGTCAA GAAAGTTTAC CAAGAGATAA TATATTATCA
    ATGGAGAATG ATAGATTACT CAGAGAGAAG AGGTTTCATG GAATTGCTTT ATTGACCAAA
    GATAATACTT TATTCAAAGA CAATGTCTCC TTAATGAAAA CAAACAAAAC ATATAATCAT
    TCAACAACTA ATGAAAAACT ACACACTGAG AGCCCAACAT CAATTGAGAA TAGTACAACA
    GACTTGCAAG ATGCCATATT AAAGGTCAAT AGTGAGATTC AAGAAGTAAC AGCTTTGATT
    CATGATGGAA CACTTTTAGG CAAAAATTCT ACATATTTGA GACTAAACCA TATGCTAAAT
    AGAACTACCT CAACAAAAAA TAAAGACATA TTTCATAGAA AAGATGAAGA TCCTATTCCA
    CAAGATGAAG AGAATACAAT CATGCCATTT TCCAAGATGT TGTTCTTGTC AGAATCTTCA
    AATTGGTTTA AAAAGACCAA TGGAAATAAT TCCTTGAACT CTGAGCAAGA ACATAGTCCA
    AAGCAATTAG TATATTTAAT GTTTAAAAAA TATGTAAAAA ATCAAAGTTT CTTGTCAGAG
    AAAAATAAAG TCACAGTAGA ACAGGATGGA TTTACAAAGA ACATAGGACT TAAAGACATG
    GCTTTTCCAC ATAATATGAG CATATTTCTT ACCACTTTGT CTAACGTACA TGAAAATGGT
    AGGCACAATC AAGAAAAAAA TATTCAGGAA GAGATAGAGA AGGAAGCACT AATTGAAGAG
    AAAGTAGTTT TGCCCCAGGT GCACGAAGCA ACTGGCTCTA AGAATTTCTT GAAAGACATA
    TTGATACTAG GCACTAGGCA AAATATAAGT TTATATGAAG TACATGTACC AGTACTTCAA
    AACATCACAT CAATAAACAA TTCAACAAAT ACAGTACAGA TTCACATGGA GCATTTCTTT
    AAAAGAAGGA AGGACAAGGA AACAAATTCA GAAGGCTTGG TAAATAAAAC CAGAGAAATG
    GTAAAAAACT ATCCAAGCCA GAAGAATATT ACTACTCAAC GTAGTAAACG GGCTTTGGGA
    CAATTCAGAC TGTCAACTCA ATGGCTTAAA ACCATAAACT GTTCAACACA GTGTATCATT
    AAACAGATAG ACCACAGCAA GGAAATGAAA AAGTTCATTA CTAAATCTTC CTTATCAGAT
    TCTTCTGTGA TTAAAAGCAC CACTCAGACA AATAGTTCTG ACTCACACAT TGTAAAAACA
    TCAGCATTTC CACCAATAGA TCTCAAAAGG AGTCCATTCC AAAACAAATT TTCTCATGTT
    CAAGCATCAT CCTACATTTA TGACTTTAAG ACAAAAAGTT CAAGAATTCA AGAAAGCAAT
    AATTTCTTAA AAGAAACCAA AATAAATAAC CCTTCTTTAG CCATTCTACC ATGGAATATG
    TTCATAGATC AAGGAAAATT TACCTCCCCA GGGAAAAGTA ACACAAACTC AGTCACATAT
    AAGAAACGTG AGAACATTAT TTTCTTGAAA CCAACTTTGC CTGAAGAATC TGGCAAAATT
    GAATTGCTTC CTCAAGTTTC CATTCAAGAG GAAGAAATTT TACCTACAGA AACTAGCCAT
    GGATCTCCTG GACACTTGAA TCTCATGAAA GAGGTCTTTC TTCAGAAAAT ACAGGGGCCT
    ACTAAATGGA ATAAAGCAAA GAGGCATGGA GAAAGTATAA AAGGTAAAAC AGAGAGCTCT
    AAAAATACTC GCTCAAAACT GCTAAATCAT CATGCTTGGG ATTATCATTA TGCTGCACAG
    ATACCAAAAG ATATGTGGAA ATCCAAAGAG AAGTCACCAG AAATTATATC CATTAAGCAA
    GAGGACACCA TTTTGTCTCT GAGGCCTCAT GGAAACAGTC ATTCAATAGG GGCAAATGAG
    AAACAAAATT GGCCTCAAAG AGAAACCACT TGGGTAAAGC AAGGCCAAAC TCAAAGGACA
    TGCTCTCAAA TCCCACCAGT GTTGAAACGA CATCAAAGGG AACTTAGTGC TTTTCAATCA
    GAACAAGAAG CAACTGACTA TGATGATGCC ATCACCATTG AAACAATCGA GGATTTTGAC
    ATTTACAGTG AGGACATAAA GCAAGGTCCC CGCAGCTTTC AACAGAAAAC AAGGCACTAT
    TTTATTGCAG CTGTGGAACG ACTCTGGGAC TATGGGATGA GTACATCTCA TGTTCTACGA
    AATAGGTATC AAAGTGACAA TGTACCTCAG TTCAAGAAAG TAGTTTTCCA GGAATTTACT
    GATGGCTCCT TTAGTCAGCC CTTATATCGT GGAGAATTAA ATGAACACCT GGGGTTGTTG
    GGCCCATATA TAAGAGCAGA AGTTGAAGAC AACATTATGG TAACTTTCAA AAACCAGGCC
    TCCCGTCCCT ACTCCTTCTA TTCTAGCCTC ATTTCTTATA AAGAAGATCA GAGAGGAGAA
    GAACCTAGAA GAAACTTTGT CAAGCCTAAT GAAACCAAAA TTTATTTTTG GAAAGTACAA
    CATCATATGG CACCCACAGA AGATGAGTTT GACTGCAAGG CCTGGGCTTA TTTCTCTGAT
    GTTGATCTTG AAAGAGATAT GCACTCGGGA TTAATTGGAC CCCTTCTGAT TTGCCACGCG
    AACACACTGA ATCCTGCTCA TGGGAGACAA GTGTCAGTAC AGGAATTTGC TCTGCTTTTC
    ACTATCTTTG ATGAGACCAA GAGCTGGTAC TTCACTGAAA ACGTGAAAAG GAACTGCAAG
    ACACCCTGCA ATTTCCAGAT GGAAGACCCC ACTTTGAAAG AGAATTATCG CTTCCATGCA
    ATCAATGGTT ATGTAATGGA TACCCTACCA GGCTTAGTAA TGGCTCAAGA TCAAAGGATT
    CGATGGTATC TTCTCAGCAT GGGCAACAAT GAGAACATCC AATCTATTCA TTTCAGTGGA
    CATGTTTTCA CTGTACGGAA AAAAGAGGAG TATAAAATGG CAGTGTACAA CCTCTACCCA
    GGTGTTTTTG AGACTCTGGA AATGATACCA TCCAGAGCTG GAATATGGCG AGTAGAATGC
    CTTATTGGCG AGCACTTACA GGCTGGGATG AGCACTCTTT TTCTGGTGTA CAGCAAGCAG
    TGTCAGATTC CTCTTGGAAT GGCTTCTGGA AGCATCCGTG ATTTCCAGAT TACAGCTTCA
    GGACATTATG GACAGTGGGC CCCAAACCTG GCAAGACTTC ATTATTCCGG ATCAATCAAT
    GCCTGGAGTA CCAAGGAGCC CTTTTCTTGG ATCAAGGTAG ATCTGTTGGC ACCAATGATT
    GTTCATGGCA TCAAGACTCA GGGTGCTCGT CAGAAATTTT CCAGCCTTTA TATCTCTCAA
    TTTATCATCA TGTATAGCCT GGATGGGAAG AAGTGGCTGA GTTATCAAGG AAATTCCACT
    GGAACCTTAA TGGTTTTCTT TGGCAATGTG GACTCATCTG GGATTAAGCA TAATAGTTTT
    AATCCTCCAA TTATTGCTCG ATATATCCGT TTGCACCCCA CTCATTCTAG CATCCGTAGT
    ACTCTTCGCA TGGAGTTGAT GGGCTGTGAT TTAAACAGTT GCAGCATACC ATTGGGAATG
    GAAAGTAAAG TAATATCAGA TACACAAATC ACTGCCTCAT CCTACTTCAC CAACATGTTT
    GCTACTTGGT CTCCTTCACA AGCTCGACTT CACCTCCAGG GAAGGACTAA TGCCTGGCGA
    CCTCAGGTGA ATGATCCAAA ACAATGGTTG CAAGTGGACT TACAAAAGAC AATGAAAGTC
    ACTGGAATAA TAACCCAGGG AGTGAAATCT CTCTTTACCA GCATGTTTGT GAAAGAGTTC
    CTTATTTCCA GCAGTCAAGA TGGCCATCAC TGGACTCAAA TTTTATACAA TGGCAAGGTA
    AAGGTTTTTC AGGGGAATCA GGACTCATCC ACACCTATGA TGAATTCTCT AGACCCACCA
    TTACTCACTC GCTATCTTCG AATTCACCCC CAGATCTGGG AGCACCAAAT TGCTCTGAGG
    CTTGAGATTC TAGGATGTGA GGCCCAGCAG CAATACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu56130
    Clone ID Related Accession (Same CDS sequence) XM_006527792.3 , XM_006527791.3 , XM_006527791.4 , XM_006527792.4
    Accession Version XM_006527792.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6801bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-21
    Organism Mus musculus(house mouse)
    Product coagulation factor VIII isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000086.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006527792.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    ATGTCAACAT CTTTTCCATT CAACACCTCC ATCATGTATA AAAAGACTGT GTTTGTAGAG 
    TACAAGGACC AGCTTTTCAA CATTGCCAAG CCCAGGCCAC CCTGGATGGG TTTGCTAGGT
    CCTACCATTT GGACTGAGGT TCATGACACA GTGGTCATTA CACTTAAAAA CATGGCTTCT
    CATCCTGTCA GTCTTCATGC TGTTGGTGTG TCCTACTGGA AAGCTTCTGA GGGAGATGAA
    TATGAAGATC AGACAAGCCA AATGGAGAAG GAAGATGATA AAGTTTTCCC TGGTGAAAGT
    CATACTTATG TTTGGCAAGT CCTGAAAGAG AATGGTCCAA TGGCCTCTGA CCCTCCATGT
    CTCACTTACT CATATATGTC TCATGTGGAT CTGGTGAAAG ATTTGAATTC AGGCCTCATT
    GGAGCTCTGC TAGTATGTAA AGAAGGCAGT CTCTCCAAAG AAAGAACACA GATGTTGTAC
    CAATTTGTAC TGCTTTTTGC TGTATTTGAT GAAGGGAAGA GCTGGCACTC AGAAACAAAC
    GACTCTTATA CACAGTCTAT GGATTCTGCA TCTGCTAGAG ACTGGCCTAA AATGCACACA
    GTCAATGGCT ATGTAAACAG GTCTCTTCCA GGTCTGATTG GATGCCATAG GAAATCAGTC
    TACTGGCACG TGATTGGAAT GGGCACCACT CCTGAAATAC ACTCAATATT CCTCGAAGGT
    CACACATTTT TTGTGAGGAA CCACCGTCAA GCTTCATTGG AGATATCACC AATAACTTTC
    CTTACTGCTC AAACACTCTT GATAGATCTT GGGCAGTTCC TACTATTTTG TCATATCTCT
    TCCCATAAAC ATGATGGCAT GGAAGCTTAT GTCAAAGTAG ATAGCTGCCC TGAGGAATCC
    CAATGGCAAA AGAAAAATAA TAATGAGGAA ATGGAAGATT ATGATGATGA TCTTTATTCA
    GAAATGGATA TGTTCACATT GGATTATGAC AGCTCTCCTT TTATCCAAAT TCGCTCGGTT
    GCTAAAAAGT ACCCTAAAAC TTGGATACAT TATATTTCTG CTGAGGAGGA AGACTGGGAC
    TATGCACCTT CAGTTCCTAC CTCGGATAAT GGAAGTTATA AAAGCCAGTA TCTGAGCAAT
    GGTCCTCATC GGATTGGTAG GAAATATAAA AAAGTCAGAT TTATAGCATA CACAGATGAA
    ACCTTTAAGA CTCGTGAAAC TATTCAGCAT GAATCAGGAC TCTTGGGACC TTTACTTTAT
    GGAGAAGTTG GAGACACACT GTTGATTATT TTTAAGAATC AAGCAAGCCG ACCATATAAC
    ATTTACCCTC ATGGAATCAC TGATGTCAGT CCTCTACATG CAAGGAGATT GCCAAGAGGT
    ATAAAGCACG TGAAGGATTT GCCAATTCAT CCAGGAGAGA TATTCAAGTA CAAGTGGACA
    GTTACAGTAG AAGATGGACC AACTAAATCA GATCCACGGT GCCTGACCCG CTATTATTCA
    AGTTTCATTA ACCCTGAGAG AGATCTAGCT TCAGGACTGA TTGGCCCTCT TCTCATCTGC
    TACAAAGAAT CTGTAGATCA AAGGGGAAAC CAGATGATGT CAGACAAAAG AAATGTCATC
    CTGTTTTCTA TATTTGATGA GAACCAAAGC TGGTACATCA CAGAGAACAT GCAACGCTTC
    CTCCCCAATG CAGCTAAAAC ACAGCCCCAG GACCCTGGGT TCCAGGCCTC CAACATCATG
    CACAGCATCA ATGGCTATGT TTTTGATAGC TTGGAGTTGA CAGTTTGTTT GCATGAGGTG
    GCATACTGGC ACATTCTCAG TGTTGGAGCA CAGACAGACT TCTTATCTAT CTTCTTCTCT
    GGATATACTT TCAAACACAA AATGGTCTAT GAAGATACAC TTACCCTGTT CCCATTCTCA
    GGAGAAACTG TCTTTATGTC GATGGAAAAC CCAGGTCTAT GGGTCTTGGG GTGTCATAAT
    TCAGACTTTC GGAAGAGAGG TATGACAGCA TTGCTGAAAG TTTCTAGTTG TGACAAGAGC
    ACTAGTGATT ATTATGAAGA AATATATGAA GATATTCCAA CACAGTTGGT GAATGAGAAC
    AATGTCATTG ATCCCAGAAG CTTCTTCCAG AATACAAATC ATCCTAATAC TAGGAAAAAG
    AAATTCAAAG ATTCCACAAT TCCAAAAAAT GATATGGAGA AGATTGAGCC TCAGTTTGAA
    GAGATAGCAG AGATGCTTAA AGTACAGAGT GTCTCAGTTA GTGACATGTT GATGCTCTTG
    GGACAGAGTC ATCCTACTCC ACATGGCTTA TTTTTATCAG ATGGCCAAGA AGCCATCTAT
    GAGGCTATTC ATGATGATCA TTCACCAAAT GCAATAGACA GCAATGAAGG CCCATCTAAA
    GTGACCCAAC TCAGGCCAGA ATCCCATCAC AGTGAGAAAA TAGTATTTAC TCCTCAGCCC
    GGCCTCCAGT TAAGATCCAA TAAAAGTTTG GAGACAACTA TAGAAGTAAA GTGGAAGAAA
    CTTGGTTTGC AAGTTTCTAG TTTGCCAAGT AATCTAATGA CTACAACAAT TCTGTCAGAC
    AATTTGAAAG CAACTTTTGA AAAGACAGAT TCTTCAGGAT TTCCAGATAT GCCAGTTCAC
    TCTAGTAGTA AATTAAGTAC TACTGCATTT GGTAAGAAAG CATATTCCCT TGTTGGGTCT
    CATGTACCTT TAAACGTGAG TGAAGAAAAT AGTGATTCCA ACATATTGGA TTCAACTTTA
    ATGTATAGTC AAGAAAGTTT ACCAAGAGAT AATATATTAT CAATGGAGAA TGATAGATTA
    CTCAGAGAGA AGAGGTTTCA TGGAATTGCT TTATTGACCA AAGATAATAC TTTATTCAAA
    GACAATGTCT CCTTAATGAA AACAAACAAA ACATATAATC ATTCAACAAC TAATGAAAAA
    CTACACACTG AGAGCCCAAC ATCAATTGAG AATAGTACAA CAGACTTGCA AGATGCCATA
    TTAAAGGTCA ATAGTGAGAT TCAAGAAGTA ACAGCTTTGA TTCATGATGG AACACTTTTA
    GGCAAAAATT CTACATATTT GAGACTAAAC CATATGCTAA ATAGAACTAC CTCAACAAAA
    AATAAAGACA TATTTCATAG AAAAGATGAA GATCCTATTC CACAAGATGA AGAGAATACA
    ATCATGCCAT TTTCCAAGAT GTTGTTCTTG TCAGAATCTT CAAATTGGTT TAAAAAGACC
    AATGGAAATA ATTCCTTGAA CTCTGAGCAA GAACATAGTC CAAAGCAATT AGTATATTTA
    ATGTTTAAAA AATATGTAAA AAATCAAAGT TTCTTGTCAG AGAAAAATAA AGTCACAGTA
    GAACAGGATG GATTTACAAA GAACATAGGA CTTAAAGACA TGGCTTTTCC ACATAATATG
    AGCATATTTC TTACCACTTT GTCTAACGTA CATGAAAATG GTAGGCACAA TCAAGAAAAA
    AATATTCAGG AAGAGATAGA GAAGGAAGCA CTAATTGAAG AGAAAGTAGT TTTGCCCCAG
    GTGCACGAAG CAACTGGCTC TAAGAATTTC TTGAAAGACA TATTGATACT AGGCACTAGG
    CAAAATATAA GTTTATATGA AGTACATGTA CCAGTACTTC AAAACATCAC ATCAATAAAC
    AATTCAACAA ATACAGTACA GATTCACATG GAGCATTTCT TTAAAAGAAG GAAGGACAAG
    GAAACAAATT CAGAAGGCTT GGTAAATAAA ACCAGAGAAA TGGTAAAAAA CTATCCAAGC
    CAGAAGAATA TTACTACTCA ACGTAGTAAA CGGGCTTTGG GACAATTCAG ACTGTCAACT
    CAATGGCTTA AAACCATAAA CTGTTCAACA CAGTGTATCA TTAAACAGAT AGACCACAGC
    AAGGAAATGA AAAAGTTCAT TACTAAATCT TCCTTATCAG ATTCTTCTGT GATTAAAAGC
    ACCACTCAGA CAAATAGTTC TGACTCACAC ATTGTAAAAA CATCAGCATT TCCACCAATA
    GATCTCAAAA GGAGTCCATT CCAAAACAAA TTTTCTCATG TTCAAGCATC ATCCTACATT
    TATGACTTTA AGACAAAAAG TTCAAGAATT CAAGAAAGCA ATAATTTCTT AAAAGAAACC
    AAAATAAATA ACCCTTCTTT AGCCATTCTA CCATGGAATA TGTTCATAGA TCAAGGAAAA
    TTTACCTCCC CAGGGAAAAG TAACACAAAC TCAGTCACAT ATAAGAAACG TGAGAACATT
    ATTTTCTTGA AACCAACTTT GCCTGAAGAA TCTGGCAAAA TTGAATTGCT TCCTCAAGTT
    TCCATTCAAG AGGAAGAAAT TTTACCTACA GAAACTAGCC ATGGATCTCC TGGACACTTG
    AATCTCATGA AAGAGGTCTT TCTTCAGAAA ATACAGGGGC CTACTAAATG GAATAAAGCA
    AAGAGGCATG GAGAAAGTAT AAAAGGTAAA ACAGAGAGCT CTAAAAATAC TCGCTCAAAA
    CTGCTAAATC ATCATGCTTG GGATTATCAT TATGCTGCAC AGATACCAAA AGATATGTGG
    AAATCCAAAG AGAAGTCACC AGAAATTATA TCCATTAAGC AAGAGGACAC CATTTTGTCT
    CTGAGGCCTC ATGGAAACAG TCATTCAATA GGGGCAAATG AGAAACAAAA TTGGCCTCAA
    AGAGAAACCA CTTGGGTAAA GCAAGGCCAA ACTCAAAGGA CATGCTCTCA AATCCCACCA
    GTGTTGAAAC GACATCAAAG GGAACTTAGT GCTTTTCAAT CAGAACAAGA AGCAACTGAC
    TATGATGATG CCATCACCAT TGAAACAATC GAGGATTTTG ACATTTACAG TGAGGACATA
    AAGCAAGGTC CCCGCAGCTT TCAACAGAAA ACAAGGCACT ATTTTATTGC AGCTGTGGAA
    CGACTCTGGG ACTATGGGAT GAGTACATCT CATGTTCTAC GAAATAGGTA TCAAAGTGAC
    AATGTACCTC AGTTCAAGAA AGTAGTTTTC CAGGAATTTA CTGATGGCTC CTTTAGTCAG
    CCCTTATATC GTGGAGAATT AAATGAACAC CTGGGGTTGT TGGGCCCATA TATAAGAGCA
    GAAGTTGAAG ACAACATTAT GGTAACTTTC AAAAACCAGG CCTCCCGTCC CTACTCCTTC
    TATTCTAGCC TCATTTCTTA TAAAGAAGAT CAGAGAGGAG AAGAACCTAG AAGAAACTTT
    GTCAAGCCTA ATGAAACCAA AATTTATTTT TGGAAAGTAC AACATCATAT GGCACCCACA
    GAAGATGAGT TTGACTGCAA GGCCTGGGCT TATTTCTCTG ATGTTGATCT TGAAAGAGAT
    ATGCACTCGG GATTAATTGG ACCCCTTCTG ATTTGCCACG CGAACACACT GAATCCTGCT
    CATGGGAGAC AAGTGTCAGT ACAGGAATTT GCTCTGCTTT TCACTATCTT TGATGAGACC
    AAGAGCTGGT ACTTCACTGA AAACGTGAAA AGGAACTGCA AGACACCCTG CAATTTCCAG
    ATGGAAGACC CCACTTTGAA AGAGAATTAT CGCTTCCATG CAATCAATGG TTATGTAATG
    GATACCCTAC CAGGCTTAGT AATGGCTCAA GATCAAAGGA TTCGATGGTA TCTTCTCAGC
    ATGGGCAACA ATGAGAACAT CCAATCTATT CATTTCAGTG GACATGTTTT CACTGTACGG
    AAAAAAGAGG AGTATAAAAT GGCAGTGTAC AACCTCTACC CAGGTGTTTT TGAGACTCTG
    GAAATGATAC CATCCAGAGC TGGAATATGG CGAGTAGAAT GCCTTATTGG CGAGCACTTA
    CAGGCTGGGA TGAGCACTCT TTTTCTGGTG TACAGCAAGC AGTGTCAGAT TCCTCTTGGA
    ATGGCTTCTG GAAGCATCCG TGATTTCCAG ATTACAGCTT CAGGACATTA TGGACAGTGG
    GCCCCAAACC TGGCAAGACT TCATTATTCC GGATCAATCA ATGCCTGGAG TACCAAGGAG
    CCCTTTTCTT GGATCAAGGT AGATCTGTTG GCACCAATGA TTGTTCATGG CATCAAGACT
    CAGGGTGCTC GTCAGAAATT TTCCAGCCTT TATATCTCTC AATTTATCAT CATGTATAGC
    CTGGATGGGA AGAAGTGGCT GAGTTATCAA GGAAATTCCA CTGGAACCTT AATGGTTTTC
    TTTGGCAATG TGGACTCATC TGGGATTAAG CATAATAGTT TTAATCCTCC AATTATTGCT
    CGATATATCC GTTTGCACCC CACTCATTCT AGCATCCGTA GTACTCTTCG CATGGAGTTG
    ATGGGCTGTG ATTTAAACAG TTGCAGCATA CCATTGGGAA TGGAAAGTAA AGTAATATCA
    GATACACAAA TCACTGCCTC ATCCTACTTC ACCAACATGT TTGCTACTTG GTCTCCTTCA
    CAAGCTCGAC TTCACCTCCA GGGAAGGACT AATGCCTGGC GACCTCAGGT GAATGATCCA
    AAACAATGGT TGCAAGTGGA CTTACAAAAG ACAATGAAAG TCACTGGAAT AATAACCCAG
    GGAGTGAAAT CTCTCTTTAC CAGCATGTTT GTGAAAGAGT TCCTTATTTC CAGCAGTCAA
    GATGGCCATC ACTGGACTCA AATTTTATAC AATGGCAAGG TAAAGGTTTT TCAGGGGAAT
    CAGGACTCAT CCACACCTAT GATGAATTCT CTAGACCCAC CATTACTCAC TCGCTATCTT
    CGAATTCACC CCCAGATCTG GGAGCACCAA ATTGCTCTGA GGCTTGAGAT TCTAGGATGT
    GAGGCCCAGC AGCAATACTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006527855.1
    CDS210..7010
    Translation

    Target ORF information:

    RefSeq Version XM_006527792.3
    Organism Mus musculus(house mouse)
    Definition Mus musculus coagulation factor VIII (F8), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006527792.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    ATGTCAACAT CTTTTCCATT CAACACCTCC ATCATGTATA AAAAGACTGT GTTTGTAGAG 
    TACAAGGACC AGCTTTTCAA CATTGCCAAG CCCAGGCCAC CCTGGATGGG TTTGCTAGGT
    CCTACCATTT GGACTGAGGT TCATGACACA GTGGTCATTA CACTTAAAAA CATGGCTTCT
    CATCCTGTCA GTCTTCATGC TGTTGGTGTG TCCTACTGGA AAGCTTCTGA GGGAGATGAA
    TATGAAGATC AGACAAGCCA AATGGAGAAG GAAGATGATA AAGTTTTCCC TGGTGAAAGT
    CATACTTATG TTTGGCAAGT CCTGAAAGAG AATGGTCCAA TGGCCTCTGA CCCTCCATGT
    CTCACTTACT CATATATGTC TCATGTGGAT CTGGTGAAAG ATTTGAATTC AGGCCTCATT
    GGAGCTCTGC TAGTATGTAA AGAAGGCAGT CTCTCCAAAG AAAGAACACA GATGTTGTAC
    CAATTTGTAC TGCTTTTTGC TGTATTTGAT GAAGGGAAGA GCTGGCACTC AGAAACAAAC
    GACTCTTATA CACAGTCTAT GGATTCTGCA TCTGCTAGAG ACTGGCCTAA AATGCACACA
    GTCAATGGCT ATGTAAACAG GTCTCTTCCA GGTCTGATTG GATGCCATAG GAAATCAGTC
    TACTGGCACG TGATTGGAAT GGGCACCACT CCTGAAATAC ACTCAATATT CCTCGAAGGT
    CACACATTTT TTGTGAGGAA CCACCGTCAA GCTTCATTGG AGATATCACC AATAACTTTC
    CTTACTGCTC AAACACTCTT GATAGATCTT GGGCAGTTCC TACTATTTTG TCATATCTCT
    TCCCATAAAC ATGATGGCAT GGAAGCTTAT GTCAAAGTAG ATAGCTGCCC TGAGGAATCC
    CAATGGCAAA AGAAAAATAA TAATGAGGAA ATGGAAGATT ATGATGATGA TCTTTATTCA
    GAAATGGATA TGTTCACATT GGATTATGAC AGCTCTCCTT TTATCCAAAT TCGCTCGGTT
    GCTAAAAAGT ACCCTAAAAC TTGGATACAT TATATTTCTG CTGAGGAGGA AGACTGGGAC
    TATGCACCTT CAGTTCCTAC CTCGGATAAT GGAAGTTATA AAAGCCAGTA TCTGAGCAAT
    GGTCCTCATC GGATTGGTAG GAAATATAAA AAAGTCAGAT TTATAGCATA CACAGATGAA
    ACCTTTAAGA CTCGTGAAAC TATTCAGCAT GAATCAGGAC TCTTGGGACC TTTACTTTAT
    GGAGAAGTTG GAGACACACT GTTGATTATT TTTAAGAATC AAGCAAGCCG ACCATATAAC
    ATTTACCCTC ATGGAATCAC TGATGTCAGT CCTCTACATG CAAGGAGATT GCCAAGAGGT
    ATAAAGCACG TGAAGGATTT GCCAATTCAT CCAGGAGAGA TATTCAAGTA CAAGTGGACA
    GTTACAGTAG AAGATGGACC AACTAAATCA GATCCACGGT GCCTGACCCG CTATTATTCA
    AGTTTCATTA ACCCTGAGAG AGATCTAGCT TCAGGACTGA TTGGCCCTCT TCTCATCTGC
    TACAAAGAAT CTGTAGATCA AAGGGGAAAC CAGATGATGT CAGACAAAAG AAATGTCATC
    CTGTTTTCTA TATTTGATGA GAACCAAAGC TGGTACATCA CAGAGAACAT GCAACGCTTC
    CTCCCCAATG CAGCTAAAAC ACAGCCCCAG GACCCTGGGT TCCAGGCCTC CAACATCATG
    CACAGCATCA ATGGCTATGT TTTTGATAGC TTGGAGTTGA CAGTTTGTTT GCATGAGGTG
    GCATACTGGC ACATTCTCAG TGTTGGAGCA CAGACAGACT TCTTATCTAT CTTCTTCTCT
    GGATATACTT TCAAACACAA AATGGTCTAT GAAGATACAC TTACCCTGTT CCCATTCTCA
    GGAGAAACTG TCTTTATGTC GATGGAAAAC CCAGGTCTAT GGGTCTTGGG GTGTCATAAT
    TCAGACTTTC GGAAGAGAGG TATGACAGCA TTGCTGAAAG TTTCTAGTTG TGACAAGAGC
    ACTAGTGATT ATTATGAAGA AATATATGAA GATATTCCAA CACAGTTGGT GAATGAGAAC
    AATGTCATTG ATCCCAGAAG CTTCTTCCAG AATACAAATC ATCCTAATAC TAGGAAAAAG
    AAATTCAAAG ATTCCACAAT TCCAAAAAAT GATATGGAGA AGATTGAGCC TCAGTTTGAA
    GAGATAGCAG AGATGCTTAA AGTACAGAGT GTCTCAGTTA GTGACATGTT GATGCTCTTG
    GGACAGAGTC ATCCTACTCC ACATGGCTTA TTTTTATCAG ATGGCCAAGA AGCCATCTAT
    GAGGCTATTC ATGATGATCA TTCACCAAAT GCAATAGACA GCAATGAAGG CCCATCTAAA
    GTGACCCAAC TCAGGCCAGA ATCCCATCAC AGTGAGAAAA TAGTATTTAC TCCTCAGCCC
    GGCCTCCAGT TAAGATCCAA TAAAAGTTTG GAGACAACTA TAGAAGTAAA GTGGAAGAAA
    CTTGGTTTGC AAGTTTCTAG TTTGCCAAGT AATCTAATGA CTACAACAAT TCTGTCAGAC
    AATTTGAAAG CAACTTTTGA AAAGACAGAT TCTTCAGGAT TTCCAGATAT GCCAGTTCAC
    TCTAGTAGTA AATTAAGTAC TACTGCATTT GGTAAGAAAG CATATTCCCT TGTTGGGTCT
    CATGTACCTT TAAACGTGAG TGAAGAAAAT AGTGATTCCA ACATATTGGA TTCAACTTTA
    ATGTATAGTC AAGAAAGTTT ACCAAGAGAT AATATATTAT CAATGGAGAA TGATAGATTA
    CTCAGAGAGA AGAGGTTTCA TGGAATTGCT TTATTGACCA AAGATAATAC TTTATTCAAA
    GACAATGTCT CCTTAATGAA AACAAACAAA ACATATAATC ATTCAACAAC TAATGAAAAA
    CTACACACTG AGAGCCCAAC ATCAATTGAG AATAGTACAA CAGACTTGCA AGATGCCATA
    TTAAAGGTCA ATAGTGAGAT TCAAGAAGTA ACAGCTTTGA TTCATGATGG AACACTTTTA
    GGCAAAAATT CTACATATTT GAGACTAAAC CATATGCTAA ATAGAACTAC CTCAACAAAA
    AATAAAGACA TATTTCATAG AAAAGATGAA GATCCTATTC CACAAGATGA AGAGAATACA
    ATCATGCCAT TTTCCAAGAT GTTGTTCTTG TCAGAATCTT CAAATTGGTT TAAAAAGACC
    AATGGAAATA ATTCCTTGAA CTCTGAGCAA GAACATAGTC CAAAGCAATT AGTATATTTA
    ATGTTTAAAA AATATGTAAA AAATCAAAGT TTCTTGTCAG AGAAAAATAA AGTCACAGTA
    GAACAGGATG GATTTACAAA GAACATAGGA CTTAAAGACA TGGCTTTTCC ACATAATATG
    AGCATATTTC TTACCACTTT GTCTAACGTA CATGAAAATG GTAGGCACAA TCAAGAAAAA
    AATATTCAGG AAGAGATAGA GAAGGAAGCA CTAATTGAAG AGAAAGTAGT TTTGCCCCAG
    GTGCACGAAG CAACTGGCTC TAAGAATTTC TTGAAAGACA TATTGATACT AGGCACTAGG
    CAAAATATAA GTTTATATGA AGTACATGTA CCAGTACTTC AAAACATCAC ATCAATAAAC
    AATTCAACAA ATACAGTACA GATTCACATG GAGCATTTCT TTAAAAGAAG GAAGGACAAG
    GAAACAAATT CAGAAGGCTT GGTAAATAAA ACCAGAGAAA TGGTAAAAAA CTATCCAAGC
    CAGAAGAATA TTACTACTCA ACGTAGTAAA CGGGCTTTGG GACAATTCAG ACTGTCAACT
    CAATGGCTTA AAACCATAAA CTGTTCAACA CAGTGTATCA TTAAACAGAT AGACCACAGC
    AAGGAAATGA AAAAGTTCAT TACTAAATCT TCCTTATCAG ATTCTTCTGT GATTAAAAGC
    ACCACTCAGA CAAATAGTTC TGACTCACAC ATTGTAAAAA CATCAGCATT TCCACCAATA
    GATCTCAAAA GGAGTCCATT CCAAAACAAA TTTTCTCATG TTCAAGCATC ATCCTACATT
    TATGACTTTA AGACAAAAAG TTCAAGAATT CAAGAAAGCA ATAATTTCTT AAAAGAAACC
    AAAATAAATA ACCCTTCTTT AGCCATTCTA CCATGGAATA TGTTCATAGA TCAAGGAAAA
    TTTACCTCCC CAGGGAAAAG TAACACAAAC TCAGTCACAT ATAAGAAACG TGAGAACATT
    ATTTTCTTGA AACCAACTTT GCCTGAAGAA TCTGGCAAAA TTGAATTGCT TCCTCAAGTT
    TCCATTCAAG AGGAAGAAAT TTTACCTACA GAAACTAGCC ATGGATCTCC TGGACACTTG
    AATCTCATGA AAGAGGTCTT TCTTCAGAAA ATACAGGGGC CTACTAAATG GAATAAAGCA
    AAGAGGCATG GAGAAAGTAT AAAAGGTAAA ACAGAGAGCT CTAAAAATAC TCGCTCAAAA
    CTGCTAAATC ATCATGCTTG GGATTATCAT TATGCTGCAC AGATACCAAA AGATATGTGG
    AAATCCAAAG AGAAGTCACC AGAAATTATA TCCATTAAGC AAGAGGACAC CATTTTGTCT
    CTGAGGCCTC ATGGAAACAG TCATTCAATA GGGGCAAATG AGAAACAAAA TTGGCCTCAA
    AGAGAAACCA CTTGGGTAAA GCAAGGCCAA ACTCAAAGGA CATGCTCTCA AATCCCACCA
    GTGTTGAAAC GACATCAAAG GGAACTTAGT GCTTTTCAAT CAGAACAAGA AGCAACTGAC
    TATGATGATG CCATCACCAT TGAAACAATC GAGGATTTTG ACATTTACAG TGAGGACATA
    AAGCAAGGTC CCCGCAGCTT TCAACAGAAA ACAAGGCACT ATTTTATTGC AGCTGTGGAA
    CGACTCTGGG ACTATGGGAT GAGTACATCT CATGTTCTAC GAAATAGGTA TCAAAGTGAC
    AATGTACCTC AGTTCAAGAA AGTAGTTTTC CAGGAATTTA CTGATGGCTC CTTTAGTCAG
    CCCTTATATC GTGGAGAATT AAATGAACAC CTGGGGTTGT TGGGCCCATA TATAAGAGCA
    GAAGTTGAAG ACAACATTAT GGTAACTTTC AAAAACCAGG CCTCCCGTCC CTACTCCTTC
    TATTCTAGCC TCATTTCTTA TAAAGAAGAT CAGAGAGGAG AAGAACCTAG AAGAAACTTT
    GTCAAGCCTA ATGAAACCAA AATTTATTTT TGGAAAGTAC AACATCATAT GGCACCCACA
    GAAGATGAGT TTGACTGCAA GGCCTGGGCT TATTTCTCTG ATGTTGATCT TGAAAGAGAT
    ATGCACTCGG GATTAATTGG ACCCCTTCTG ATTTGCCACG CGAACACACT GAATCCTGCT
    CATGGGAGAC AAGTGTCAGT ACAGGAATTT GCTCTGCTTT TCACTATCTT TGATGAGACC
    AAGAGCTGGT ACTTCACTGA AAACGTGAAA AGGAACTGCA AGACACCCTG CAATTTCCAG
    ATGGAAGACC CCACTTTGAA AGAGAATTAT CGCTTCCATG CAATCAATGG TTATGTAATG
    GATACCCTAC CAGGCTTAGT AATGGCTCAA GATCAAAGGA TTCGATGGTA TCTTCTCAGC
    ATGGGCAACA ATGAGAACAT CCAATCTATT CATTTCAGTG GACATGTTTT CACTGTACGG
    AAAAAAGAGG AGTATAAAAT GGCAGTGTAC AACCTCTACC CAGGTGTTTT TGAGACTCTG
    GAAATGATAC CATCCAGAGC TGGAATATGG CGAGTAGAAT GCCTTATTGG CGAGCACTTA
    CAGGCTGGGA TGAGCACTCT TTTTCTGGTG TACAGCAAGC AGTGTCAGAT TCCTCTTGGA
    ATGGCTTCTG GAAGCATCCG TGATTTCCAG ATTACAGCTT CAGGACATTA TGGACAGTGG
    GCCCCAAACC TGGCAAGACT TCATTATTCC GGATCAATCA ATGCCTGGAG TACCAAGGAG
    CCCTTTTCTT GGATCAAGGT AGATCTGTTG GCACCAATGA TTGTTCATGG CATCAAGACT
    CAGGGTGCTC GTCAGAAATT TTCCAGCCTT TATATCTCTC AATTTATCAT CATGTATAGC
    CTGGATGGGA AGAAGTGGCT GAGTTATCAA GGAAATTCCA CTGGAACCTT AATGGTTTTC
    TTTGGCAATG TGGACTCATC TGGGATTAAG CATAATAGTT TTAATCCTCC AATTATTGCT
    CGATATATCC GTTTGCACCC CACTCATTCT AGCATCCGTA GTACTCTTCG CATGGAGTTG
    ATGGGCTGTG ATTTAAACAG TTGCAGCATA CCATTGGGAA TGGAAAGTAA AGTAATATCA
    GATACACAAA TCACTGCCTC ATCCTACTTC ACCAACATGT TTGCTACTTG GTCTCCTTCA
    CAAGCTCGAC TTCACCTCCA GGGAAGGACT AATGCCTGGC GACCTCAGGT GAATGATCCA
    AAACAATGGT TGCAAGTGGA CTTACAAAAG ACAATGAAAG TCACTGGAAT AATAACCCAG
    GGAGTGAAAT CTCTCTTTAC CAGCATGTTT GTGAAAGAGT TCCTTATTTC CAGCAGTCAA
    GATGGCCATC ACTGGACTCA AATTTTATAC AATGGCAAGG TAAAGGTTTT TCAGGGGAAT
    CAGGACTCAT CCACACCTAT GATGAATTCT CTAGACCCAC CATTACTCAC TCGCTATCTT
    CGAATTCACC CCCAGATCTG GGAGCACCAA ATTGCTCTGA GGCTTGAGAT TCTAGGATGT
    GAGGCCCAGC AGCAATACTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu56130
    Clone ID Related Accession (Same CDS sequence) XM_006527792.3 , XM_006527791.3 , XM_006527791.4 , XM_006527792.4
    Accession Version XM_006527791.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6801bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-21
    Organism Mus musculus(house mouse)
    Product coagulation factor VIII isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000086.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006527791.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    ATGTCAACAT CTTTTCCATT CAACACCTCC ATCATGTATA AAAAGACTGT GTTTGTAGAG 
    TACAAGGACC AGCTTTTCAA CATTGCCAAG CCCAGGCCAC CCTGGATGGG TTTGCTAGGT
    CCTACCATTT GGACTGAGGT TCATGACACA GTGGTCATTA CACTTAAAAA CATGGCTTCT
    CATCCTGTCA GTCTTCATGC TGTTGGTGTG TCCTACTGGA AAGCTTCTGA GGGAGATGAA
    TATGAAGATC AGACAAGCCA AATGGAGAAG GAAGATGATA AAGTTTTCCC TGGTGAAAGT
    CATACTTATG TTTGGCAAGT CCTGAAAGAG AATGGTCCAA TGGCCTCTGA CCCTCCATGT
    CTCACTTACT CATATATGTC TCATGTGGAT CTGGTGAAAG ATTTGAATTC AGGCCTCATT
    GGAGCTCTGC TAGTATGTAA AGAAGGCAGT CTCTCCAAAG AAAGAACACA GATGTTGTAC
    CAATTTGTAC TGCTTTTTGC TGTATTTGAT GAAGGGAAGA GCTGGCACTC AGAAACAAAC
    GACTCTTATA CACAGTCTAT GGATTCTGCA TCTGCTAGAG ACTGGCCTAA AATGCACACA
    GTCAATGGCT ATGTAAACAG GTCTCTTCCA GGTCTGATTG GATGCCATAG GAAATCAGTC
    TACTGGCACG TGATTGGAAT GGGCACCACT CCTGAAATAC ACTCAATATT CCTCGAAGGT
    CACACATTTT TTGTGAGGAA CCACCGTCAA GCTTCATTGG AGATATCACC AATAACTTTC
    CTTACTGCTC AAACACTCTT GATAGATCTT GGGCAGTTCC TACTATTTTG TCATATCTCT
    TCCCATAAAC ATGATGGCAT GGAAGCTTAT GTCAAAGTAG ATAGCTGCCC TGAGGAATCC
    CAATGGCAAA AGAAAAATAA TAATGAGGAA ATGGAAGATT ATGATGATGA TCTTTATTCA
    GAAATGGATA TGTTCACATT GGATTATGAC AGCTCTCCTT TTATCCAAAT TCGCTCGGTT
    GCTAAAAAGT ACCCTAAAAC TTGGATACAT TATATTTCTG CTGAGGAGGA AGACTGGGAC
    TATGCACCTT CAGTTCCTAC CTCGGATAAT GGAAGTTATA AAAGCCAGTA TCTGAGCAAT
    GGTCCTCATC GGATTGGTAG GAAATATAAA AAAGTCAGAT TTATAGCATA CACAGATGAA
    ACCTTTAAGA CTCGTGAAAC TATTCAGCAT GAATCAGGAC TCTTGGGACC TTTACTTTAT
    GGAGAAGTTG GAGACACACT GTTGATTATT TTTAAGAATC AAGCAAGCCG ACCATATAAC
    ATTTACCCTC ATGGAATCAC TGATGTCAGT CCTCTACATG CAAGGAGATT GCCAAGAGGT
    ATAAAGCACG TGAAGGATTT GCCAATTCAT CCAGGAGAGA TATTCAAGTA CAAGTGGACA
    GTTACAGTAG AAGATGGACC AACTAAATCA GATCCACGGT GCCTGACCCG CTATTATTCA
    AGTTTCATTA ACCCTGAGAG AGATCTAGCT TCAGGACTGA TTGGCCCTCT TCTCATCTGC
    TACAAAGAAT CTGTAGATCA AAGGGGAAAC CAGATGATGT CAGACAAAAG AAATGTCATC
    CTGTTTTCTA TATTTGATGA GAACCAAAGC TGGTACATCA CAGAGAACAT GCAACGCTTC
    CTCCCCAATG CAGCTAAAAC ACAGCCCCAG GACCCTGGGT TCCAGGCCTC CAACATCATG
    CACAGCATCA ATGGCTATGT TTTTGATAGC TTGGAGTTGA CAGTTTGTTT GCATGAGGTG
    GCATACTGGC ACATTCTCAG TGTTGGAGCA CAGACAGACT TCTTATCTAT CTTCTTCTCT
    GGATATACTT TCAAACACAA AATGGTCTAT GAAGATACAC TTACCCTGTT CCCATTCTCA
    GGAGAAACTG TCTTTATGTC GATGGAAAAC CCAGGTCTAT GGGTCTTGGG GTGTCATAAT
    TCAGACTTTC GGAAGAGAGG TATGACAGCA TTGCTGAAAG TTTCTAGTTG TGACAAGAGC
    ACTAGTGATT ATTATGAAGA AATATATGAA GATATTCCAA CACAGTTGGT GAATGAGAAC
    AATGTCATTG ATCCCAGAAG CTTCTTCCAG AATACAAATC ATCCTAATAC TAGGAAAAAG
    AAATTCAAAG ATTCCACAAT TCCAAAAAAT GATATGGAGA AGATTGAGCC TCAGTTTGAA
    GAGATAGCAG AGATGCTTAA AGTACAGAGT GTCTCAGTTA GTGACATGTT GATGCTCTTG
    GGACAGAGTC ATCCTACTCC ACATGGCTTA TTTTTATCAG ATGGCCAAGA AGCCATCTAT
    GAGGCTATTC ATGATGATCA TTCACCAAAT GCAATAGACA GCAATGAAGG CCCATCTAAA
    GTGACCCAAC TCAGGCCAGA ATCCCATCAC AGTGAGAAAA TAGTATTTAC TCCTCAGCCC
    GGCCTCCAGT TAAGATCCAA TAAAAGTTTG GAGACAACTA TAGAAGTAAA GTGGAAGAAA
    CTTGGTTTGC AAGTTTCTAG TTTGCCAAGT AATCTAATGA CTACAACAAT TCTGTCAGAC
    AATTTGAAAG CAACTTTTGA AAAGACAGAT TCTTCAGGAT TTCCAGATAT GCCAGTTCAC
    TCTAGTAGTA AATTAAGTAC TACTGCATTT GGTAAGAAAG CATATTCCCT TGTTGGGTCT
    CATGTACCTT TAAACGTGAG TGAAGAAAAT AGTGATTCCA ACATATTGGA TTCAACTTTA
    ATGTATAGTC AAGAAAGTTT ACCAAGAGAT AATATATTAT CAATGGAGAA TGATAGATTA
    CTCAGAGAGA AGAGGTTTCA TGGAATTGCT TTATTGACCA AAGATAATAC TTTATTCAAA
    GACAATGTCT CCTTAATGAA AACAAACAAA ACATATAATC ATTCAACAAC TAATGAAAAA
    CTACACACTG AGAGCCCAAC ATCAATTGAG AATAGTACAA CAGACTTGCA AGATGCCATA
    TTAAAGGTCA ATAGTGAGAT TCAAGAAGTA ACAGCTTTGA TTCATGATGG AACACTTTTA
    GGCAAAAATT CTACATATTT GAGACTAAAC CATATGCTAA ATAGAACTAC CTCAACAAAA
    AATAAAGACA TATTTCATAG AAAAGATGAA GATCCTATTC CACAAGATGA AGAGAATACA
    ATCATGCCAT TTTCCAAGAT GTTGTTCTTG TCAGAATCTT CAAATTGGTT TAAAAAGACC
    AATGGAAATA ATTCCTTGAA CTCTGAGCAA GAACATAGTC CAAAGCAATT AGTATATTTA
    ATGTTTAAAA AATATGTAAA AAATCAAAGT TTCTTGTCAG AGAAAAATAA AGTCACAGTA
    GAACAGGATG GATTTACAAA GAACATAGGA CTTAAAGACA TGGCTTTTCC ACATAATATG
    AGCATATTTC TTACCACTTT GTCTAACGTA CATGAAAATG GTAGGCACAA TCAAGAAAAA
    AATATTCAGG AAGAGATAGA GAAGGAAGCA CTAATTGAAG AGAAAGTAGT TTTGCCCCAG
    GTGCACGAAG CAACTGGCTC TAAGAATTTC TTGAAAGACA TATTGATACT AGGCACTAGG
    CAAAATATAA GTTTATATGA AGTACATGTA CCAGTACTTC AAAACATCAC ATCAATAAAC
    AATTCAACAA ATACAGTACA GATTCACATG GAGCATTTCT TTAAAAGAAG GAAGGACAAG
    GAAACAAATT CAGAAGGCTT GGTAAATAAA ACCAGAGAAA TGGTAAAAAA CTATCCAAGC
    CAGAAGAATA TTACTACTCA ACGTAGTAAA CGGGCTTTGG GACAATTCAG ACTGTCAACT
    CAATGGCTTA AAACCATAAA CTGTTCAACA CAGTGTATCA TTAAACAGAT AGACCACAGC
    AAGGAAATGA AAAAGTTCAT TACTAAATCT TCCTTATCAG ATTCTTCTGT GATTAAAAGC
    ACCACTCAGA CAAATAGTTC TGACTCACAC ATTGTAAAAA CATCAGCATT TCCACCAATA
    GATCTCAAAA GGAGTCCATT CCAAAACAAA TTTTCTCATG TTCAAGCATC ATCCTACATT
    TATGACTTTA AGACAAAAAG TTCAAGAATT CAAGAAAGCA ATAATTTCTT AAAAGAAACC
    AAAATAAATA ACCCTTCTTT AGCCATTCTA CCATGGAATA TGTTCATAGA TCAAGGAAAA
    TTTACCTCCC CAGGGAAAAG TAACACAAAC TCAGTCACAT ATAAGAAACG TGAGAACATT
    ATTTTCTTGA AACCAACTTT GCCTGAAGAA TCTGGCAAAA TTGAATTGCT TCCTCAAGTT
    TCCATTCAAG AGGAAGAAAT TTTACCTACA GAAACTAGCC ATGGATCTCC TGGACACTTG
    AATCTCATGA AAGAGGTCTT TCTTCAGAAA ATACAGGGGC CTACTAAATG GAATAAAGCA
    AAGAGGCATG GAGAAAGTAT AAAAGGTAAA ACAGAGAGCT CTAAAAATAC TCGCTCAAAA
    CTGCTAAATC ATCATGCTTG GGATTATCAT TATGCTGCAC AGATACCAAA AGATATGTGG
    AAATCCAAAG AGAAGTCACC AGAAATTATA TCCATTAAGC AAGAGGACAC CATTTTGTCT
    CTGAGGCCTC ATGGAAACAG TCATTCAATA GGGGCAAATG AGAAACAAAA TTGGCCTCAA
    AGAGAAACCA CTTGGGTAAA GCAAGGCCAA ACTCAAAGGA CATGCTCTCA AATCCCACCA
    GTGTTGAAAC GACATCAAAG GGAACTTAGT GCTTTTCAAT CAGAACAAGA AGCAACTGAC
    TATGATGATG CCATCACCAT TGAAACAATC GAGGATTTTG ACATTTACAG TGAGGACATA
    AAGCAAGGTC CCCGCAGCTT TCAACAGAAA ACAAGGCACT ATTTTATTGC AGCTGTGGAA
    CGACTCTGGG ACTATGGGAT GAGTACATCT CATGTTCTAC GAAATAGGTA TCAAAGTGAC
    AATGTACCTC AGTTCAAGAA AGTAGTTTTC CAGGAATTTA CTGATGGCTC CTTTAGTCAG
    CCCTTATATC GTGGAGAATT AAATGAACAC CTGGGGTTGT TGGGCCCATA TATAAGAGCA
    GAAGTTGAAG ACAACATTAT GGTAACTTTC AAAAACCAGG CCTCCCGTCC CTACTCCTTC
    TATTCTAGCC TCATTTCTTA TAAAGAAGAT CAGAGAGGAG AAGAACCTAG AAGAAACTTT
    GTCAAGCCTA ATGAAACCAA AATTTATTTT TGGAAAGTAC AACATCATAT GGCACCCACA
    GAAGATGAGT TTGACTGCAA GGCCTGGGCT TATTTCTCTG ATGTTGATCT TGAAAGAGAT
    ATGCACTCGG GATTAATTGG ACCCCTTCTG ATTTGCCACG CGAACACACT GAATCCTGCT
    CATGGGAGAC AAGTGTCAGT ACAGGAATTT GCTCTGCTTT TCACTATCTT TGATGAGACC
    AAGAGCTGGT ACTTCACTGA AAACGTGAAA AGGAACTGCA AGACACCCTG CAATTTCCAG
    ATGGAAGACC CCACTTTGAA AGAGAATTAT CGCTTCCATG CAATCAATGG TTATGTAATG
    GATACCCTAC CAGGCTTAGT AATGGCTCAA GATCAAAGGA TTCGATGGTA TCTTCTCAGC
    ATGGGCAACA ATGAGAACAT CCAATCTATT CATTTCAGTG GACATGTTTT CACTGTACGG
    AAAAAAGAGG AGTATAAAAT GGCAGTGTAC AACCTCTACC CAGGTGTTTT TGAGACTCTG
    GAAATGATAC CATCCAGAGC TGGAATATGG CGAGTAGAAT GCCTTATTGG CGAGCACTTA
    CAGGCTGGGA TGAGCACTCT TTTTCTGGTG TACAGCAAGC AGTGTCAGAT TCCTCTTGGA
    ATGGCTTCTG GAAGCATCCG TGATTTCCAG ATTACAGCTT CAGGACATTA TGGACAGTGG
    GCCCCAAACC TGGCAAGACT TCATTATTCC GGATCAATCA ATGCCTGGAG TACCAAGGAG
    CCCTTTTCTT GGATCAAGGT AGATCTGTTG GCACCAATGA TTGTTCATGG CATCAAGACT
    CAGGGTGCTC GTCAGAAATT TTCCAGCCTT TATATCTCTC AATTTATCAT CATGTATAGC
    CTGGATGGGA AGAAGTGGCT GAGTTATCAA GGAAATTCCA CTGGAACCTT AATGGTTTTC
    TTTGGCAATG TGGACTCATC TGGGATTAAG CATAATAGTT TTAATCCTCC AATTATTGCT
    CGATATATCC GTTTGCACCC CACTCATTCT AGCATCCGTA GTACTCTTCG CATGGAGTTG
    ATGGGCTGTG ATTTAAACAG TTGCAGCATA CCATTGGGAA TGGAAAGTAA AGTAATATCA
    GATACACAAA TCACTGCCTC ATCCTACTTC ACCAACATGT TTGCTACTTG GTCTCCTTCA
    CAAGCTCGAC TTCACCTCCA GGGAAGGACT AATGCCTGGC GACCTCAGGT GAATGATCCA
    AAACAATGGT TGCAAGTGGA CTTACAAAAG ACAATGAAAG TCACTGGAAT AATAACCCAG
    GGAGTGAAAT CTCTCTTTAC CAGCATGTTT GTGAAAGAGT TCCTTATTTC CAGCAGTCAA
    GATGGCCATC ACTGGACTCA AATTTTATAC AATGGCAAGG TAAAGGTTTT TCAGGGGAAT
    CAGGACTCAT CCACACCTAT GATGAATTCT CTAGACCCAC CATTACTCAC TCGCTATCTT
    CGAATTCACC CCCAGATCTG GGAGCACCAA ATTGCTCTGA GGCTTGAGAT TCTAGGATGT
    GAGGCCCAGC AGCAATACTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006527854.1
    CDS216..7016
    Translation

    Target ORF information:

    RefSeq Version XM_006527791.3
    Organism Mus musculus(house mouse)
    Definition Mus musculus coagulation factor VIII (F8), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006527791.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    ATGTCAACAT CTTTTCCATT CAACACCTCC ATCATGTATA AAAAGACTGT GTTTGTAGAG 
    TACAAGGACC AGCTTTTCAA CATTGCCAAG CCCAGGCCAC CCTGGATGGG TTTGCTAGGT
    CCTACCATTT GGACTGAGGT TCATGACACA GTGGTCATTA CACTTAAAAA CATGGCTTCT
    CATCCTGTCA GTCTTCATGC TGTTGGTGTG TCCTACTGGA AAGCTTCTGA GGGAGATGAA
    TATGAAGATC AGACAAGCCA AATGGAGAAG GAAGATGATA AAGTTTTCCC TGGTGAAAGT
    CATACTTATG TTTGGCAAGT CCTGAAAGAG AATGGTCCAA TGGCCTCTGA CCCTCCATGT
    CTCACTTACT CATATATGTC TCATGTGGAT CTGGTGAAAG ATTTGAATTC AGGCCTCATT
    GGAGCTCTGC TAGTATGTAA AGAAGGCAGT CTCTCCAAAG AAAGAACACA GATGTTGTAC
    CAATTTGTAC TGCTTTTTGC TGTATTTGAT GAAGGGAAGA GCTGGCACTC AGAAACAAAC
    GACTCTTATA CACAGTCTAT GGATTCTGCA TCTGCTAGAG ACTGGCCTAA AATGCACACA
    GTCAATGGCT ATGTAAACAG GTCTCTTCCA GGTCTGATTG GATGCCATAG GAAATCAGTC
    TACTGGCACG TGATTGGAAT GGGCACCACT CCTGAAATAC ACTCAATATT CCTCGAAGGT
    CACACATTTT TTGTGAGGAA CCACCGTCAA GCTTCATTGG AGATATCACC AATAACTTTC
    CTTACTGCTC AAACACTCTT GATAGATCTT GGGCAGTTCC TACTATTTTG TCATATCTCT
    TCCCATAAAC ATGATGGCAT GGAAGCTTAT GTCAAAGTAG ATAGCTGCCC TGAGGAATCC
    CAATGGCAAA AGAAAAATAA TAATGAGGAA ATGGAAGATT ATGATGATGA TCTTTATTCA
    GAAATGGATA TGTTCACATT GGATTATGAC AGCTCTCCTT TTATCCAAAT TCGCTCGGTT
    GCTAAAAAGT ACCCTAAAAC TTGGATACAT TATATTTCTG CTGAGGAGGA AGACTGGGAC
    TATGCACCTT CAGTTCCTAC CTCGGATAAT GGAAGTTATA AAAGCCAGTA TCTGAGCAAT
    GGTCCTCATC GGATTGGTAG GAAATATAAA AAAGTCAGAT TTATAGCATA CACAGATGAA
    ACCTTTAAGA CTCGTGAAAC TATTCAGCAT GAATCAGGAC TCTTGGGACC TTTACTTTAT
    GGAGAAGTTG GAGACACACT GTTGATTATT TTTAAGAATC AAGCAAGCCG ACCATATAAC
    ATTTACCCTC ATGGAATCAC TGATGTCAGT CCTCTACATG CAAGGAGATT GCCAAGAGGT
    ATAAAGCACG TGAAGGATTT GCCAATTCAT CCAGGAGAGA TATTCAAGTA CAAGTGGACA
    GTTACAGTAG AAGATGGACC AACTAAATCA GATCCACGGT GCCTGACCCG CTATTATTCA
    AGTTTCATTA ACCCTGAGAG AGATCTAGCT TCAGGACTGA TTGGCCCTCT TCTCATCTGC
    TACAAAGAAT CTGTAGATCA AAGGGGAAAC CAGATGATGT CAGACAAAAG AAATGTCATC
    CTGTTTTCTA TATTTGATGA GAACCAAAGC TGGTACATCA CAGAGAACAT GCAACGCTTC
    CTCCCCAATG CAGCTAAAAC ACAGCCCCAG GACCCTGGGT TCCAGGCCTC CAACATCATG
    CACAGCATCA ATGGCTATGT TTTTGATAGC TTGGAGTTGA CAGTTTGTTT GCATGAGGTG
    GCATACTGGC ACATTCTCAG TGTTGGAGCA CAGACAGACT TCTTATCTAT CTTCTTCTCT
    GGATATACTT TCAAACACAA AATGGTCTAT GAAGATACAC TTACCCTGTT CCCATTCTCA
    GGAGAAACTG TCTTTATGTC GATGGAAAAC CCAGGTCTAT GGGTCTTGGG GTGTCATAAT
    TCAGACTTTC GGAAGAGAGG TATGACAGCA TTGCTGAAAG TTTCTAGTTG TGACAAGAGC
    ACTAGTGATT ATTATGAAGA AATATATGAA GATATTCCAA CACAGTTGGT GAATGAGAAC
    AATGTCATTG ATCCCAGAAG CTTCTTCCAG AATACAAATC ATCCTAATAC TAGGAAAAAG
    AAATTCAAAG ATTCCACAAT TCCAAAAAAT GATATGGAGA AGATTGAGCC TCAGTTTGAA
    GAGATAGCAG AGATGCTTAA AGTACAGAGT GTCTCAGTTA GTGACATGTT GATGCTCTTG
    GGACAGAGTC ATCCTACTCC ACATGGCTTA TTTTTATCAG ATGGCCAAGA AGCCATCTAT
    GAGGCTATTC ATGATGATCA TTCACCAAAT GCAATAGACA GCAATGAAGG CCCATCTAAA
    GTGACCCAAC TCAGGCCAGA ATCCCATCAC AGTGAGAAAA TAGTATTTAC TCCTCAGCCC
    GGCCTCCAGT TAAGATCCAA TAAAAGTTTG GAGACAACTA TAGAAGTAAA GTGGAAGAAA
    CTTGGTTTGC AAGTTTCTAG TTTGCCAAGT AATCTAATGA CTACAACAAT TCTGTCAGAC
    AATTTGAAAG CAACTTTTGA AAAGACAGAT TCTTCAGGAT TTCCAGATAT GCCAGTTCAC
    TCTAGTAGTA AATTAAGTAC TACTGCATTT GGTAAGAAAG CATATTCCCT TGTTGGGTCT
    CATGTACCTT TAAACGTGAG TGAAGAAAAT AGTGATTCCA ACATATTGGA TTCAACTTTA
    ATGTATAGTC AAGAAAGTTT ACCAAGAGAT AATATATTAT CAATGGAGAA TGATAGATTA
    CTCAGAGAGA AGAGGTTTCA TGGAATTGCT TTATTGACCA AAGATAATAC TTTATTCAAA
    GACAATGTCT CCTTAATGAA AACAAACAAA ACATATAATC ATTCAACAAC TAATGAAAAA
    CTACACACTG AGAGCCCAAC ATCAATTGAG AATAGTACAA CAGACTTGCA AGATGCCATA
    TTAAAGGTCA ATAGTGAGAT TCAAGAAGTA ACAGCTTTGA TTCATGATGG AACACTTTTA
    GGCAAAAATT CTACATATTT GAGACTAAAC CATATGCTAA ATAGAACTAC CTCAACAAAA
    AATAAAGACA TATTTCATAG AAAAGATGAA GATCCTATTC CACAAGATGA AGAGAATACA
    ATCATGCCAT TTTCCAAGAT GTTGTTCTTG TCAGAATCTT CAAATTGGTT TAAAAAGACC
    AATGGAAATA ATTCCTTGAA CTCTGAGCAA GAACATAGTC CAAAGCAATT AGTATATTTA
    ATGTTTAAAA AATATGTAAA AAATCAAAGT TTCTTGTCAG AGAAAAATAA AGTCACAGTA
    GAACAGGATG GATTTACAAA GAACATAGGA CTTAAAGACA TGGCTTTTCC ACATAATATG
    AGCATATTTC TTACCACTTT GTCTAACGTA CATGAAAATG GTAGGCACAA TCAAGAAAAA
    AATATTCAGG AAGAGATAGA GAAGGAAGCA CTAATTGAAG AGAAAGTAGT TTTGCCCCAG
    GTGCACGAAG CAACTGGCTC TAAGAATTTC TTGAAAGACA TATTGATACT AGGCACTAGG
    CAAAATATAA GTTTATATGA AGTACATGTA CCAGTACTTC AAAACATCAC ATCAATAAAC
    AATTCAACAA ATACAGTACA GATTCACATG GAGCATTTCT TTAAAAGAAG GAAGGACAAG
    GAAACAAATT CAGAAGGCTT GGTAAATAAA ACCAGAGAAA TGGTAAAAAA CTATCCAAGC
    CAGAAGAATA TTACTACTCA ACGTAGTAAA CGGGCTTTGG GACAATTCAG ACTGTCAACT
    CAATGGCTTA AAACCATAAA CTGTTCAACA CAGTGTATCA TTAAACAGAT AGACCACAGC
    AAGGAAATGA AAAAGTTCAT TACTAAATCT TCCTTATCAG ATTCTTCTGT GATTAAAAGC
    ACCACTCAGA CAAATAGTTC TGACTCACAC ATTGTAAAAA CATCAGCATT TCCACCAATA
    GATCTCAAAA GGAGTCCATT CCAAAACAAA TTTTCTCATG TTCAAGCATC ATCCTACATT
    TATGACTTTA AGACAAAAAG TTCAAGAATT CAAGAAAGCA ATAATTTCTT AAAAGAAACC
    AAAATAAATA ACCCTTCTTT AGCCATTCTA CCATGGAATA TGTTCATAGA TCAAGGAAAA
    TTTACCTCCC CAGGGAAAAG TAACACAAAC TCAGTCACAT ATAAGAAACG TGAGAACATT
    ATTTTCTTGA AACCAACTTT GCCTGAAGAA TCTGGCAAAA TTGAATTGCT TCCTCAAGTT
    TCCATTCAAG AGGAAGAAAT TTTACCTACA GAAACTAGCC ATGGATCTCC TGGACACTTG
    AATCTCATGA AAGAGGTCTT TCTTCAGAAA ATACAGGGGC CTACTAAATG GAATAAAGCA
    AAGAGGCATG GAGAAAGTAT AAAAGGTAAA ACAGAGAGCT CTAAAAATAC TCGCTCAAAA
    CTGCTAAATC ATCATGCTTG GGATTATCAT TATGCTGCAC AGATACCAAA AGATATGTGG
    AAATCCAAAG AGAAGTCACC AGAAATTATA TCCATTAAGC AAGAGGACAC CATTTTGTCT
    CTGAGGCCTC ATGGAAACAG TCATTCAATA GGGGCAAATG AGAAACAAAA TTGGCCTCAA
    AGAGAAACCA CTTGGGTAAA GCAAGGCCAA ACTCAAAGGA CATGCTCTCA AATCCCACCA
    GTGTTGAAAC GACATCAAAG GGAACTTAGT GCTTTTCAAT CAGAACAAGA AGCAACTGAC
    TATGATGATG CCATCACCAT TGAAACAATC GAGGATTTTG ACATTTACAG TGAGGACATA
    AAGCAAGGTC CCCGCAGCTT TCAACAGAAA ACAAGGCACT ATTTTATTGC AGCTGTGGAA
    CGACTCTGGG ACTATGGGAT GAGTACATCT CATGTTCTAC GAAATAGGTA TCAAAGTGAC
    AATGTACCTC AGTTCAAGAA AGTAGTTTTC CAGGAATTTA CTGATGGCTC CTTTAGTCAG
    CCCTTATATC GTGGAGAATT AAATGAACAC CTGGGGTTGT TGGGCCCATA TATAAGAGCA
    GAAGTTGAAG ACAACATTAT GGTAACTTTC AAAAACCAGG CCTCCCGTCC CTACTCCTTC
    TATTCTAGCC TCATTTCTTA TAAAGAAGAT CAGAGAGGAG AAGAACCTAG AAGAAACTTT
    GTCAAGCCTA ATGAAACCAA AATTTATTTT TGGAAAGTAC AACATCATAT GGCACCCACA
    GAAGATGAGT TTGACTGCAA GGCCTGGGCT TATTTCTCTG ATGTTGATCT TGAAAGAGAT
    ATGCACTCGG GATTAATTGG ACCCCTTCTG ATTTGCCACG CGAACACACT GAATCCTGCT
    CATGGGAGAC AAGTGTCAGT ACAGGAATTT GCTCTGCTTT TCACTATCTT TGATGAGACC
    AAGAGCTGGT ACTTCACTGA AAACGTGAAA AGGAACTGCA AGACACCCTG CAATTTCCAG
    ATGGAAGACC CCACTTTGAA AGAGAATTAT CGCTTCCATG CAATCAATGG TTATGTAATG
    GATACCCTAC CAGGCTTAGT AATGGCTCAA GATCAAAGGA TTCGATGGTA TCTTCTCAGC
    ATGGGCAACA ATGAGAACAT CCAATCTATT CATTTCAGTG GACATGTTTT CACTGTACGG
    AAAAAAGAGG AGTATAAAAT GGCAGTGTAC AACCTCTACC CAGGTGTTTT TGAGACTCTG
    GAAATGATAC CATCCAGAGC TGGAATATGG CGAGTAGAAT GCCTTATTGG CGAGCACTTA
    CAGGCTGGGA TGAGCACTCT TTTTCTGGTG TACAGCAAGC AGTGTCAGAT TCCTCTTGGA
    ATGGCTTCTG GAAGCATCCG TGATTTCCAG ATTACAGCTT CAGGACATTA TGGACAGTGG
    GCCCCAAACC TGGCAAGACT TCATTATTCC GGATCAATCA ATGCCTGGAG TACCAAGGAG
    CCCTTTTCTT GGATCAAGGT AGATCTGTTG GCACCAATGA TTGTTCATGG CATCAAGACT
    CAGGGTGCTC GTCAGAAATT TTCCAGCCTT TATATCTCTC AATTTATCAT CATGTATAGC
    CTGGATGGGA AGAAGTGGCT GAGTTATCAA GGAAATTCCA CTGGAACCTT AATGGTTTTC
    TTTGGCAATG TGGACTCATC TGGGATTAAG CATAATAGTT TTAATCCTCC AATTATTGCT
    CGATATATCC GTTTGCACCC CACTCATTCT AGCATCCGTA GTACTCTTCG CATGGAGTTG
    ATGGGCTGTG ATTTAAACAG TTGCAGCATA CCATTGGGAA TGGAAAGTAA AGTAATATCA
    GATACACAAA TCACTGCCTC ATCCTACTTC ACCAACATGT TTGCTACTTG GTCTCCTTCA
    CAAGCTCGAC TTCACCTCCA GGGAAGGACT AATGCCTGGC GACCTCAGGT GAATGATCCA
    AAACAATGGT TGCAAGTGGA CTTACAAAAG ACAATGAAAG TCACTGGAAT AATAACCCAG
    GGAGTGAAAT CTCTCTTTAC CAGCATGTTT GTGAAAGAGT TCCTTATTTC CAGCAGTCAA
    GATGGCCATC ACTGGACTCA AATTTTATAC AATGGCAAGG TAAAGGTTTT TCAGGGGAAT
    CAGGACTCAT CCACACCTAT GATGAATTCT CTAGACCCAC CATTACTCAC TCGCTATCTT
    CGAATTCACC CCCAGATCTG GGAGCACCAA ATTGCTCTGA GGCTTGAGAT TCTAGGATGT
    GAGGCCCAGC AGCAATACTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.