Muc4 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Muc4
Entrez Gene ID	140474
Full Name	mucin 4
Synonyms	4933405I11Rik,Asgp
General protein information	Preferred Names mucin 4 Names mucin-4 ascites sialoglycoprotein mucin 4, ASGP pancreatic adenocarcinoma mucin testis mucin
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 16 Map Location: 16\|16 B3
Summary	The major constituents of mucus, the viscous secretion that covers epithelial surfaces such as those in the trachea, colon, and cervix, are highly glycosylated proteins called mucins. These glycoproteins play important roles in the protection of the epithelial cells and have been implicated in epithelial renewal and differentiation. This gene encodes an integral membrane glycoprotein found on the cell surface. A large 5' exon encodes at least 15 tandem repeats of 124-126 amino acids. [provided by RefSeq, Jul 2008].

mRNA and Protein(s)

mRNA	Protein	Name
NM_080457.3	NP_536705.3	mucin-4 precursor

Pathways from BioSystems

Homologies

Bos taurus (cattle)	MUC4	XP_002684865.3
Mus musculus (house mouse)	Muc4	NP_536705.3
Rattus norvegicus (Norway rat)	Muc4	XP_006248533.1
Canis lupus familiaris (dog)	MUC4	XP_545147.2

Gene Ontology
Bibliography

Related articles in PubMed

Interferon Regulatory Factor 6 Is Necessary for Salivary Glands and Pancreas Development.
Metwalli KA, Do MA, Nguyen K, Mallick S, Kin K, Farokhnia N, Jun G, Fakhouri WD
Journal of dental research97(2)226-236(2018 Feb)

Membrane Mucin Muc4 promotes blood cell association with tumor cells and mediates efficient metastasis in a mouse model of breast cancer.
Rowson-Hodel AR, Wald JH, Hatakeyama J, O'Neal WK, Stonebraker JR, VanderVorst K, Saldana MJ, Borowsky AD, Sweeney C, Carraway KL 3rd
Oncogene37(2)197-207(2018 Jan)

Quantitative proteomic profiling of the extracellular matrix of pancreatic islets during the angiogenic switch and insulinoma progression.
Naba A, Clauser KR, Mani DR, Carr SA, Hynes RO
Scientific reports740495(2017 Jan)

APC haploinsufficiency coupled with p53 loss sufficiently induces mucinous cystic neoplasms and invasive pancreatic carcinoma in mice.
Kuo TL, Weng CC, Kuo KK, Chen CY, Wu DC, Hung WC, Cheng KH
Oncogene35(17)2223-34(2016 04)

The mucin MUC4 is a transcriptional and post-transcriptional target of K-ras oncogene in pancreatic cancer. Implication of MAPK/AP-1, NF-?B and RalB signaling pathways.
Vasseur R, Skrypek N, Duch?ne B, Renaud F, Mart?nez-Maqueda D, Vincent A, Porchet N, Van Seuningen I, Jonckheere N
Biochimica et biophysica acta1849(12)1375-84(2015 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

the role of Muc4 in driving intestinal inflammation and inflammation-associated tumorigenesis
Title: Mice deficient in Muc4 are resistant to experimental colitis and colitis-associated colorectal cancer.

Using the Pdx1-Cre; LStopL-K-rasG12D mouse model of pancreatic carcinogenesis, in vivo early Muc4 neo-expression of in pancreatic intraepithelial neoplastic lesions induced by mutated hK-ras is correlated with the ERK, JNK and NF-kappaB signaling pathways.
Title: The mucin MUC4 is a transcriptional and post-transcriptional target of K-ras oncogene in pancreatic cancer. Implication of MAPK/AP-1, NF-?B and RalB signaling pathways.

Results show that while MUC4 is highly expressed in the mouse uterus, it is not a major mucin in normal human endometrium. Rather, MUC4 is a potential marker of endometrial adenocarcinoma in a subset of patients.
Title: Cytokine stimulation of MUC4 expression in human female reproductive tissue carcinoma cell lines and endometrial cancer.

Data show that Muc4 is upregulated and interacts with erbB2 in gallbladders from BK5.erbB2 mice suggesting its important role during gallbladder carcinogenesis and/or cancer growth by potentiating erbB2 signaling.
Title: Interaction of Muc4 and ErbB2 in a transgenic mouse model of gallbladder carcinoma: potential pathobiological implications.

This study delineates the association and the downstream mechanisms of MUC4-regulated elevation of lipocalin-2 and its clinical significance for prognosis of pancreatic cancer.
Title: MUC4-mediated regulation of acute phase protein lipocalin 2 through HER2/AKT/NF-?B signaling in pancreatic cancer.

The following Muc4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Muc4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu20119	NM_080457.3 Latest version!	Mus musculus mucin 4 (Muc4), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Muc4 ( NM_080457.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_536705.3 Mus musculus mucin 4 (Muc4), mRNA.

Price & Availability↑

CloneID	OMu20119
Clone ID Related Accession (Same CDS sequence)	NM_080457.3
Accession Version	NM_080457.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 10413bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-11
Organism	Mus musculus(house mouse)
Product	mucin-4 precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC161755.3 and AC139244.4. On Feb 13, 2008 this sequence version replaced NM_080457.2. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. The RefSeq includes an unresolved tandem repeat and may not correctly represent the genomic sequence. Two deletions were introduced in the repeat region compared to the reference genome to maintain the reading frame compared to mouse and human protein data. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMN00849377, SAMN00849378 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## CDS uses downstream in-frame AUG :: upstream AUG and CDS extension is not conserved ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGAGAGGGC CTCACTGGAG AGTTCCCTGG CTGTGTCTGA GCTGCCTGTA TTCTTGCCTC 
CTCTTGCTAC CTGATGCTCT AGCAACAACA TCAACACAGA CACCCATGTC CCTTTCCAGC 
AGCACCAGAA CATCCCAAAT GAGCTCACAA GCATCGACCT CATCAACATC TTCTGACAGA 
AGGACAAGTA AAACAGAACA AACATCAACA AGGGATACTC CAAGTTCCAT AACAACAGTT 
TCTCAGAGCC ATCATACTAC AAGCATGGAG ACCAGCAAAC CTCAAACCAC CACAACCACC 
GAGGTGACCA CATCAACTCC TTCAGCCTCC TCACGTGACC AAATACAGAC AGAGACAAGT 
TCTCAAAGAA CAATCTCTCC TGATGGAACA ACCACCTCAC ATGCTCCCAG TATCAGCAGC 
TCAGCTCCAA GTACAACACA CATGTTAACC ACAACATCCT CCACAGAAAG TACCTCAGTA 
GACTCAGGAC ACACAACAGC AATAACAACT CAAGGTTTAA CACCTGCCAC CGCACAAGTC 
TCACTGACAC CTTCATCCCA GAATATGTCA ACAGTGTCAA CACCCATCAC CTCAACTCTT 
ACTCAGAGAC AACACACTGG AAGCAAGCAG ACCAGCAGCA AATCTCAAGT CAACATAGTC 
ACATCAACTC TTTCAACCTC TACAAGTGAC TCAACTCCTG CACAGACAAT GTCTCAAGTG 
ACATCATCTT CAGATAAAAG AACCAAGCCA TCTACTTCTG GTGTCAGCAG TACATCACTA 
ACTACAACAG AAGTGCTCAC CCAAACATCC TCTACAGACT CTGCTCCAGG AAACACAACT 
CTGAGGATTA CTCAGAACTC TACAACACAT ACCACGAAAG TCTCAACAAC ATCTACACCA 
CAAAAACTGT CACCAGTGTC AACACTCATC AATTCAAGTC AAAAAATGTC AACCCTTCCT 
CAGAACCAAC ACACTGAAAG CATGGACACC AGCAGACAAC CTCAAACCAC CACAACCATA 
GAGGTGACCA CATCAACCCC TTCAGCCTCC TCACTTCACC AAATTCAGAC AGAGACAAAT 
TCCCCAAAAA CAATCTCTCC TGGTGAGACA ACCACCTCAC ATGCTCCGAA CATGAGAAGC 
TCACCACCAA AGACATCACA GATACTAACC ACAATGCCCT CCACAAAAAG TACCTCAGTA 
GACACAAAAC AAACAAAGGC AATAACTACT AAAGTTTCTA CACCTGACAC CACACAGGTC 
TCGATGACAC CTTCATCCCA GAAATTGCCA ACACACAGCA CGTCTACTCA GGAATTAACA 
TCTTCTTACA GCCAACATAT TCAAAGCAAG GGGACCAGCA GCAAATCTCA AACAACCACA 
AACACCAAGG TGAACACATC AACTCCTTCA GCCTCCTCAC GTGACAAAAT TCAGACAGAG 
ACAAGTTCCC AAAGAACAAA CTCTCCTGGC GAGAAAAGGA CATCACATGC TCCCAGCATG 
AGCAGCTCAG CTCCAAGTAC AACACACATG TTATCCACAA CATCCTCCAA TCAAAGTACC 
TCAGTAGACA CAGGACAAAC AACATCAGTA ACAGCTCAAG GTTCTACACC TGCCATCACA 
CAGACCTCAT TGACACCTTC ATCCCAGAAT ACGTCAACAG TGTCAACACC CATCACCTCT 
ACTCACAAAT TGTCAACCCT TTCTCAGAGC CAACACACTG GAAGCAAGGG GACCAGCAGC 
AATCCTCAAA CTACCACCAC CACAGAGGTG ACCACATCAA CTCCTTCAGC CACCACCCAT 
GACCAAATTC AGACAGAGAC AAGTTCCCAA AACACAATCT CTCCTGGTGA GACAACCACC 
TCATATGCTC CCATCATGAG CAGCTCAGCT CCAAGTACAA CACATATGTT ATCCACAACA 
TCCTCCACTC AAAGTACCTC AGTAGACACA AGACACACTA CAACATTAAC GAATCAAGGA 
TCTACACCTG CCACCACACA GGTCTCACCT TCATCCCAGA ACATGTCAAC AGTGTCAGCA 
CCCATCACCT CAACTCAAAT TTTGTCAACC TTTCCTCAGA GCCAACACAC TGGAAGCAAG 
GGGACCAGCA GCAATCCTCA AACTACCACC ACCCCAGTGG TGACCACATC AAATCCTTCA 
GCCACCTCAC GTGACCAAAT TCAGACAGAG ACAAGTTCCC AAAGAACAAT CTCTCCTGGT 
GAGACAACCA CCTCATATGC TTCCATCATG AGCAGCTCAG CTCCAAGTAC AACACACATG 
TTAACCACAA CATCCTCCAC TCAAAGTACC TCAGTAGACA CAAGACACAC AATAGCAGTA 
AGGACTCAAG GTTCTACACC TGCCACCACA CAGGTCTCAC CTTCATCCCA GAACATGTCA 
ACAGTGTCAA CACCCATCAC CTCAACTCAA ATTTTGTCAA CCCTTCCTCA GAGCCAACAC 
ACTGGAAGCA AGGGGACCAG CAGCAATCCT CAAACTACCA CAAGCCCAGT GGTAACCACA 
TCAACTCCTT CAGGCACCTC AGGTGACCAA ATTCAGACAG AGACAAGTTC CCAAAGAACA 
ATCTCTCCCG GCAAGACAAC CACTTCACAT GCTCTCAACA TAAACAGCTC AGCTCCAAGT 
ACAACACACA TGTTATCCAC AACATCCTCC ACTCAAAGTA CCTCAGGAGA CACAAGACAC 
ACAACAGCAG GAAGGACTCA AGGTTCTACA CCTGCCACCA CACAGGTCTC ACCTTCATCC 
CAGAACATGT CAACAGTGTC AGCACCCATC ACCTCAATTC AAATGTTGTC AACCCTTCCT 
CAGAGCCAAC ACACTGAAAG CAAGAGTACC AGCACCAATC CTCAAACTAC CACCACCCCA 
GAGGTGACCA CATCAAATCC TTCAGCCACC TCACATGACC AAATTGAGAC AGAAACAAGT 
TCCCAAAGAA CAATCTCTCC TGGTGAGACA ACCACCTCAT ATGCTCCCAT CATGAGCAGC 
TCAGCTCCAA GTACAACACA CATGTTATCC ACAACATCCT CCACTCAAAG TACCTCAGTA 
GACACAAGAA ACACTACAAC ATTAACGACT CAAGGATCTA CACCTGCCAC CACACAGGTC 
TCACCTTCAT CCAAGAACAT GTCAACAGTG TCAACACCAA TCACCTCTAC TCACAAATTG 
TCAACCCTTC CTCAGAGCCA ACACACTGGA AGCAATGGGA CCAGCAGCTC AAGTTCTACA 
CCTGCCACAC ACAGGTCTCA CCTTCATCCC AACATGTCAA CAGTGTCAAC ACCCATCACC 
ACTACTCACA AATTGTCAAC CCTTTCTCAG AGCCAACACA CTGGAAGCAA GGGGACCAGC 
AGCAATCCTC AAACTACCAC AACGCCAGTG ATGACCACAT CAACTCCTTC AGCCACCACC 
CATGACCAAA TTCAGACAGA GACAAGTTCT CAAAGAACAA TCTCTCATGG TGAGACAACC 
ACCTCATATG CTCCCATCAT GAGCAGCTCA GCTCCAAGTA CAACACACAT GTTATCAACA 
GCATCCTCTA CTCAAATTAC CTCAGTAGAC ACAAGACACA CAACAGCAAT AACAACTCAA 
GGTTCTACAC CTGCCACCAC ACAGGTCTCA CCTTCATCCC AGAACATGTC AACAGTGTCA 
GCACCCATCA CCTCAATTCA AATTTTGTCA ACCCTTCCTC AGAGCCAACA CACTGGAAGC 
AAGGGGACCA GCACCAATCC TCAAACTACC ACCACCCCAG AGGTGACCAC ATCAACTCCT 
TCAGCCACCT CACGTGACCA AATTCAGACA GAGACAAGTT CCCAAAGAAC AATCTCTCCT 
GGTGAGACAA CCACCTCACA TGCTCCCATC ATGAGCAGCT CAGCTCCAAG TACAACACAC 
ATGTTATCCA CAACATCCTC CACTCAAAGT ACCTCAGTAG ACACAAGACA CACAACAGCA 
GGAAGGACTC AAGGTTCTAC ACCTGCCACC ACACAGGTCT CACCTTCCTC CCAGAACATG 
ACAACCACCT CACATGCCCT CATGAGCAGC TCAGCTCCAA GTACAACACA CATGTTATCC 
ACAACATCTT CCACTCAAAG TACCTCAGTA GATACAAGAC ACACTACAAC AGTAACGACT 
CAAGGTTCTA CACCTGCCAC CACACAGGTC TTACCTTCAT CCCAGAACAT GTCAACAGTG 
TCAGCTCCCA TCACCTCAAC TCAAATTTTG TCAACCCTTC CTCAGAGCCA ACACACTGGA 
AGCAAGGGGA CCAGCACCAA TCCTCAAACT ACCACCAATG CAGAGGTGAC CACATCAACT 
CCTTCAGCCA CCTCACATGA CCAAATTGAG ACAGAAACAA GTTCCCAAAG AACAATCTCT 
CCTGGTGAGA CAACCACCTC ACATGCTCCC AACATGAGTA GTTCAGCTCC AAGTACAACA 
CACATGTTAT CCTCAACATC CTCCACTCAA ATTACCTCAG TAGACACAGG ACACACATCA 
GCAGGAAGGA CTCAAGGTTC TACACCTGCC ACCACACAGG TCTCACCTTC ATCCCAGAAC 
ATGTCAACAG TGTCAGCACC CATCACCTCA ACTCATATTT TGTCAACCCT TCCAAAGAGC 
CAACACACTG GAAGCAAGGG GACCAGCAGC AATCCTCAAA CTACCATAAC CCCAGTGGTA 
ACCACATCAA CTCCTTCAGC CTCCTCCCGT GACCAAATTC AGACAGAGAC TAGTTTCCAA 
AGAACAATCT CTCCTGGCGA GACAACCACC TCACATGCTC CCAGCATGAG CAGCTCAGCT 
CCAAGTTCAA CACACATGTT ATCAACAGCA TCCTCTACTC AAATTACCTC AGTAGACACA 
AGACACACAA CAGCAATAAC AACTCAAGGT TCTACACCTG CCACCACACA GGTCTCACCT 
TCATCCCAGA ACATGTCAAC AGTGTCAGCA CCCATCACCT CAATTCAAAT GTTGTCAACC 
CTTCCTCAGA GCCAACACAC TGAAAGCAAG AGTACCAGCA CCAATCCTCA AACTACCACC 
ACCCCCAGAG TGACCACATC AACTCCTTCA GCCTCCTCAC GTGACCAAAT TCAGACAGAG 
ACAAGTTCCC AAAGAACAAT CTCTCCCGGC AAGACAACCA CCTCACATGT TCCCAACATG 
AACAGCTCAG CTCCAAGTAC AACACACATC TTATCCACAA CATCCTCCAT TCAAAGTACC 
TCAGGAGATA CAAGACACAC AACAGCAGTA AGGACTCAAG GTTCTACACC TGCCACCACA 
CAGGTCTCAC TGGCACCTTC ATCCCAGAAC ATGTCAACAC TGTCAGCACC CATCACATCA 
CCTCAACATT TTTCAACCCT TCCTCAGAAC CAACACACTG GAAGCATGGG GACCAGCAGC 
AATCCTCAAA GTACCACCAT CCCAGAGGTG ACCACATCAA CTCTTTCAGC TTCCTCACGT 
GACCAAGTTC AGACAGAGAC AAGTTCCCAA AGAACAATCT CTCCTGGTGA GACAACCACC 
TCACATGCTT CCAGCTTGAG CAGTTCAGCT CCAAGTTCAA CACACATGTT ATCCACAGCA 
TCCTCCACTG AAATTACCTC AGGAGACACA AGACACACAA CAGCAATAGT GACTCAAGGT 
TCTACACCTG CCACCACACA GACCACATTG ACACCTTCGT CCCAGAATAT GTCAACAGTG 
TCAACACCCA TCACTTCTAC TCACAAATTG TCACCCCTTC CTCAGAGCCA ACACACTGAA 
AACATGGGGA CCAGCAGCAA TCCTCAAACT ACCACCACCC CAGAAGTGAC CACATCAACT 
CCTTCAGCCA CCTCCTATGA CCAAATTCAG ACAGAGACAA GTTTCCAAAG AACAATCTCT 
CCTGGTGAGA CAACCACTTC ACATGCTCCC AGCATGAGCA ACTCAGCTCC AAGTTCAACA 
CACAAGTTAT CAACAGCATC CTCCACTGAA ATTACCTCAG TAGACACAAG ACACACAATA 
GCAATAACGA CTCAAGGTTC TACACTTGCC ACCACACAGA CCTCACTGAC ACCTTCATCC 
CAGAACATGT CAACAGTGTC AGCACCCATC ACCTCATCTC AAATTTTGTC AACTCTTCGT 
CAGAGCCAAC ACACTGGAAG CAAGGGGACC AGCAGCAATC ATCAGACTAC CACTACCCCT 
GTGGTGACCA CATCAACTTC TTCAGCCACC TCACGTGACC AAATTCAGAC AGAGACAAGT 
TCCCTAAGAA CAATCTCTCC TGATGGAACG ACCACCTCAC ATGCTTCCAG CATGAGCAGT 
TCATCTCCAA ATACAACACA CTTGTTAATC ACTACATCCT CCACAGAAAG TACCTCAGTA 
GACACAGGAT ACTCAACAGT AATAACGACT CATGGTTCTA CACTTGCCAC CACCCAGGTC 
TCATTGACAC CTTCATCCCA GAATATGTCA ACAGTGTCAA TGCCCAGCAC TTCTTCACAG 
GAATTAACAT CACTTCCTCA GAGACAACAC ACTGGAAACA TGGAGACCAG CAGCCAACCT 
CAGAATATCA CCCCTACTGT GGTGACCACA TCAACTCTTT TATCATTCTC AAGGGGATCC 
ACTGAATTGC AAACCATGTC TTGGGGAACG TCCTCCTCAG GGACAATAAA TACCCTATCT 
ACTCCCGTCA GGAATACCTC ACCTGCATCC ACATCAGGCA TCCTCACCAG CACATTGACC 
ACCTCAGGAA ACACAGGGTA CACAGGTGTA ACTAGAAGCC TCGGTGTGAT CACATCAAGA 
GTCACAAGCA CAAGTCTACC TGGAAAGTCC ACAGTAGTGC ATAGCACCCC TGCTCAGCCA 
TTGTCTGCAC ATTCTCAGAG TCACCAGACG TATGGCACGG GGACACCCAG CATGTCTCAA 
ACCAGCATCC TCCCAGACAT GACCTCAGAA AAGCGTGTTG CCTCTTCACC TGGACCCACT 
GTGACAGAGT CCTTTTCTCA TGTGTCATCC TCCTCAGGAT TGACTACGAA GACAGACAAT 
GACAGAAACA CAGCAGTCTC AGCCACCTCC TCGACCCTTA CTAGCCCCAG CCCAACCACA 
GCCTCCAGGT CAACAGTGCC CCTGCCTTCT CTTTTACCAG ATCAGGGCAT TTCCCTCTTC 
CCCTACGGGT CTGAAGTCGG AGACCAGAAT TTGTTTGCCA GGACTGTGGA TTTTAACTCA 
CCAATCTTCA AGATCCTGAT TGGCTTTCCC CTCGGTTCCT CCCTGCGGGA TTCCTTCTAC 
GTTACAGATA ATGGTCAGAT CATTTTCCCA GAGTCAGACT ATGACGTCTT CTCCTACCCC 
AACCCACCTC AAAGAGGCTT CACCGGAAGG GAGCGCGTGG CCATGGTGGC CCCGTTCTGG 
GCTGATGCTG ATTTCTCTAG CTCCAGGGGA GCCATATTTT ACCAGGAATA TGTCACATTT 
TACAATGAAC ACCATCAGCT AATCCGGGAA GTGGAGACTT TGATTAATGA TTTCACAAGT 
AGCTGGGGCT ACAGAGCCAA GTGGACACTG AAGGTCACCT GGGTCAATGT TCCTGCCTAT 
ACTGCCCAAG AGAGCTTTGG GACCAATACC TACCAAGCCA TCCTCTCCAC AGATGGGAGC 
AGGTCCTATG CCCTGTTTCT CTACCAGAAC AGTGGCATGA GGTGGGATGT GACCCAGGAA 
CCATACAACC GGGTGCTCAT GGGCTTCTCC AGTGGAGATG GGTATTTTGA AAACAGCCCG 
CTGACATTCC GGCCAGCAAT GGAGAAGTAC CGTCCAGATC GATTCCTGAA TTCCAAATTA 
GGCATCCGGG GACTGCAGGT CTACAGGCTG CACAGGGAGG AGCGGCCCAA CTACCGGCTC 
AAATGTCTGC AGTGGCTGGA GAGTCAGCCT CAGCAGCCCA GCTGGGGCTG GAGCTCAGTT 
TCCTGCCCTT GCTCCTGGCA GCAGGGACAA CGGGACTTTC GGTTCAGGCC CATCAACCCA 
GGTTGGTGGG ACAGGCAGCT GTGCAGCTTC TCCTCCGGGC GAGGGGGTGT GTGCTGCAGC 
TATGGTGCCT GGGGAGAGTT TCGAGAAGGC TGGAGAATGC ACAGTCCTTG GCAGTTTGAT 
GAGGAGCAGG AGGCACAGAA CTGGTGCTGC CAATGGAACG ACAAGCCCTC GTTCTGTGTC 
TGGTACCAGC TTAGGCGGCC CCGTGTCAGC TGTGCTGGGT ACAGGCCCCC ACGGCCCGCT 
TGGACATTTG GTGATCCCCA CATCACCACT TTGGATAATG CCAACTTCAC CTTCAACGGG 
CTGGGAGACT TTCTGCTGGT CCAGGCCCAG GACAGAAATT CTTCCTTCCT GCTGGAAGGC 
CGCACTGCCC AGACTGGCAC TGCCAAGGCC ACTAACTTCA TTGCCTTTGC GGCTCAATAC 
AACACCAGCA GCCTGAAGTC TCCCATCACG GTTCAGTGGT TTCTTGAGCC CAGTGACAAA 
ATCCGAGTCG TATACAATAA CCAGACTGTG GCCTTTAACA CCAGAGACAC TGAAGTCTTA 
CCCATATTCA ATACCACCGG TGTTCTGCTG ACCCAAAATG GCTCCCAAGT CTCAGCCAAC 
TTTGATGGGA CAGTGACTAT CTCTGTGATT GCTCGCTCCA ACATCCTTCA TGCCTCCTCC 
AGCCTATCAG AGGAATATCG AAACCACACA GAGGGCCTTC TGGGAGTGTG GAATGACAAT 
CCAGAAGATG ACTTCAGGAT GCCCAATGGC TCCACCATTC CCTCAAACAG CTCTGAGGAG 
ACCCTTTTCT ACTATGGAAT GACATGGCAC GTCAACGGAA CAGGCCTCCT TGGGATAAGG 
GCAGACCCGC TGCCTACCAA GTTCACCCCC ATCTTTCTGT CTCAACTGTT GAATCAGAGT 
GCCTCAGGTG AAGACTTGGC CTCTGGGTGC AAAGGCGACA GAAAGTGCAT GTTTGACATC 
CTGGCCACGG GAAACAGAAC TATTGGACAA AGCACCAATT CCATCCTTAA TGAGTTCCAA 
CATATGAATG ACACCCTCAA TCAGTACCCA CCCTCTATCA ACTGCAGCAG CAAGATTCAA 
GCCTACAAGG GGCAGACAGT GACCACTGAG ATCACCAGCA ACTCTAAGGA TGCCACACTC 
AGCCTCTCCA AGAAATGTAG TGGCTTTAAG CTCTTTGAAA ACGGGAGTTT GCAGTGGACA 
CCAACATCCC CAGAAGCGTG TACCCTGGAG ATTCTAGCGA GGGATGTCAG GACTAACTTG 
TCATGGGTAC TCCAGCCCAA GACCGTGGCT TGCTTCTGCA GTAAGGAGGA GCAGTGTTTG 
TACAATGAAA CCAGCAAAGA GGGCAATTCT TCCCTTGAGG TGACCAGCTG CAAGTGTGAT 
GGGGACACCT TTGGCCGCTT GTGTGAACGC TCCAAGGACC CGTGTGATGA GCCATGCTTC 
CCTAACGTGA ACTGCATTCC TGGGAAGGGC TGTGAGGCCT GCCCTCCAAA CACAACTGGA 
GATGGACGTC ATTGTGCAGC TCTGGAGGAC TCCTGCCCAA ACCGTTCCTG TCCCATGAAT 
TACTGCTATA ACAATGGCCA TTGCGACATC TCTGAGGCTC CAGGCTGCCA GCCCACTTGC 
ACCTGTCCCC CTGCCTTCAC TGATAACCGC TGCTTCCTGG CTGGGAACAG TTTCACTCCC 
ACCATCTCTA TGGAGCTTCC CTTAAGGACC ATCGTGCTCT CTCTCAGGGA GGATGAAAAT 
GCCTCCGCCG CTGACGTCAA CGCCTCGGTG GCGAACATAC TAGAGAACCT GGACATGCGG 
GCTTTTTTCT CCAACAGCTT AGTGGAACTG ATACGAACCT CTCCCGGAGC ACAGCCCTCG 
AGCAAGTCCA TTCATCACTG GAAGGTCACC TCCCACTTCA AGTACCGTCC CAGGGGCCCG 
CTCATCCACT ATCTGAACAA CCAGCTGATA GGCGCCGTGA TGGAGGCCTT CCTCCTGCAG 
GCTCGGCAGG AGAGGCAGAA GAGGAGTGGA GAAGCCAGGA AGGACGTCCA CTTCTTCCCC 
ATCTCGAGGG CAGATGTCCA GGACCAGATG GCTCTGAACC TAAGTATGCT GGAGGAGTAC 
TTCACATGTG ATGGCTACAA AGGCTACCAC CTGGTCTACA GCCCACAGGA TGGTGTCACC 
TGTGTGTCCC CATGTAGTGA GGGCTACTGT CACAATGGAG GCCAATGCAA GCACCTGCCA 
GATGGGCCCC AATGCAGCTG CGCATCCTTC ACCATCTACT CATCCTCGGG CGAACACTGT 
GAGCACCTAA GTGTGAAACT TGGGGCATTC TACGGGATCC TCTTCGGAAC CCTGGGCGCC 
CTCTTGCTAC TGGGGATCTT AGCATTTATG ATCTTTCACT TCTGCGGCTG CTCCAAGAAC 
AAGTTCTCCT ACCCTTTGGA CTCAGAATTG TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_536705.3
CDS	495..10907
Translation	MRGPHWRVPWLCLSCLYSCLLLLPDALATTSTQTPMSLSSSTRTSQMSSQASTSSTSSDRRTSKTEQTSTRDTPSSITTVSQSHHTTSMETSKPQTTTTTEVTTSTPSASSRDQIQTETSSQRTISPDGTTTSHAPSISSSAPSTTHMLTTTSSTESTSVDSGHTTAITTQGLTPATAQVSLTPSSQNMSTVSTPITSTLTQRQHTGSKQTSSKSQVNIVTSTLSTSTSDSTPAQTMSQVTSSSDKRTKPSTSGVSSTSLTTTEVLTQTSSTDSAPGNTTLRITQNSTTHTTKVSTTSTPQKLSPVSTLINSSQKMSTLPQNQHTESMDTSRQPQTTTTIEVTTSTPSASSLHQIQTETNSPKTISPGETTTSHAPNMRSSPPKTSQILTTMPSTKSTSVDTKQTKAITTKVSTPDTTQVSMTPSSQKLPTHSTSTQELTSSYSQHIQSKGTSSKSQTTTNTKVNTSTPSASSRDKIQTETSSQRTNSPGEKRTSHAPSMSSSAPSTTHMLSTTSSNQSTSVDTGQTTSVTAQGSTPAITQTSLTPSSQNTSTVSTPITSTHKLSTLSQSQHTGSKGTSSNPQTTTTTEVTTSTPSATTHDQIQTETSSQNTISPGETTTSYAPIMSSSAPSTTHMLSTTSSTQSTSVDTRHTTTLTNQGSTPATTQVSPSSQNMSTVSAPITSTQILSTFPQSQHTGSKGTSSNPQTTTTPVVTTSNPSATSRDQIQTETSSQRTISPGETTTSYASIMSSSAPSTTHMLTTTSSTQSTSVDTRHTIAVRTQGSTPATTQVSPSSQNMSTVSTPITSTQILSTLPQSQHTGSKGTSSNPQTTTSPVVTTSTPSGTSGDQIQTETSSQRTISPGKTTTSHALNINSSAPSTTHMLSTTSSTQSTSGDTRHTTAGRTQGSTPATTQVSPSSQNMSTVSAPITSIQMLSTLPQSQHTESKSTSTNPQTTTTPEVTTSNPSATSHDQIETETSSQRTISPGETTTSYAPIMSSSAPSTTHMLSTTSSTQSTSVDTRNTTTLTTQGSTPATTQVSPSSKNMSTVSTPITSTHKLSTLPQSQHTGSNGTSSSSSTPATHRSHLHPNMSTVSTPITTTHKLSTLSQSQHTGSKGTSSNPQTTTTPVMTTSTPSATTHDQIQTETSSQRTISHGETTTSYAPIMSSSAPSTTHMLSTASSTQITSVDTRHTTAITTQGSTPATTQVSPSSQNMSTVSAPITSIQILSTLPQSQHTGSKGTSTNPQTTTTPEVTTSTPSATSRDQIQTETSSQRTISPGETTTSHAPIMSSSAPSTTHMLSTTSSTQSTSVDTRHTTAGRTQGSTPATTQVSPSSQNMTTTSHALMSSSAPSTTHMLSTTSSTQSTSVDTRHTTTVTTQGSTPATTQVLPSSQNMSTVSAPITSTQILSTLPQSQHTGSKGTSTNPQTTTNAEVTTSTPSATSHDQIETETSSQRTISPGETTTSHAPNMSSSAPSTTHMLSSTSSTQITSVDTGHTSAGRTQGSTPATTQVSPSSQNMSTVSAPITSTHILSTLPKSQHTGSKGTSSNPQTTITPVVTTSTPSASSRDQIQTETSFQRTISPGETTTSHAPSMSSSAPSSTHMLSTASSTQITSVDTRHTTAITTQGSTPATTQVSPSSQNMSTVSAPITSIQMLSTLPQSQHTESKSTSTNPQTTTTPRVTTSTPSASSRDQIQTETSSQRTISPGKTTTSHVPNMNSSAPSTTHILSTTSSIQSTSGDTRHTTAVRTQGSTPATTQVSLAPSSQNMSTLSAPITSPQHFSTLPQNQHTGSMGTSSNPQSTTIPEVTTSTLSASSRDQVQTETSSQRTISPGETTTSHASSLSSSAPSSTHMLSTASSTEITSGDTRHTTAIVTQGSTPATTQTTLTPSSQNMSTVSTPITSTHKLSPLPQSQHTENMGTSSNPQTTTTPEVTTSTPSATSYDQIQTETSFQRTISPGETTTSHAPSMSNSAPSSTHKLSTASSTEITSVDTRHTIAITTQGSTLATTQTSLTPSSQNMSTVSAPITSSQILSTLRQSQHTGSKGTSSNHQTTTTPVVTTSTSSATSRDQIQTETSSLRTISPDGTTTSHASSMSSSSPNTTHLLITTSSTESTSVDTGYSTVITTHGSTLATTQVSLTPSSQNMSTVSMPSTSSQELTSLPQRQHTGNMETSSQPQNITPTVVTTSTLLSFSRGSTELQTMSWGTSSSGTINTLSTPVRNTSPASTSGILTSTLTTSGNTGYTGVTRSLGVITSRVTSTSLPGKSTVVHSTPAQPLSAHSQSHQTYGTGTPSMSQTSILPDMTSEKRVASSPGPTVTESFSHVSSSSGLTTKTDNDRNTAVSATSSTLTSPSPTTASRSTVPLPSLLPDQGISLFPYGSEVGDQNLFARTVDFNSPIFKILIGFPLGSSLRDSFYVTDNGQIIFPESDYDVFSYPNPPQRGFTGRERVAMVAPFWADADFSSSRGAIFYQEYVTFYNEHHQLIREVETLINDFTSSWGYRAKWTLKVTWVNVPAYTAQESFGTNTYQAILSTDGSRSYALFLYQNSGMRWDVTQEPYNRVLMGFSSGDGYFENSPLTFRPAMEKYRPDRFLNSKLGIRGLQVYRLHREERPNYRLKCLQWLESQPQQPSWGWSSVSCPCSWQQGQRDFRFRPINPGWWDRQLCSFSSGRGGVCCSYGAWGEFREGWRMHSPWQFDEEQEAQNWCCQWNDKPSFCVWYQLRRPRVSCAGYRPPRPAWTFGDPHITTLDNANFTFNGLGDFLLVQAQDRNSSFLLEGRTAQTGTAKATNFIAFAAQYNTSSLKSPITVQWFLEPSDKIRVVYNNQTVAFNTRDTEVLPIFNTTGVLLTQNGSQVSANFDGTVTISVIARSNILHASSSLSEEYRNHTEGLLGVWNDNPEDDFRMPNGSTIPSNSSEETLFYYGMTWHVNGTGLLGIRADPLPTKFTPIFLSQLLNQSASGEDLASGCKGDRKCMFDILATGNRTIGQSTNSILNEFQHMNDTLNQYPPSINCSSKIQAYKGQTVTTEITSNSKDATLSLSKKCSGFKLFENGSLQWTPTSPEACTLEILARDVRTNLSWVLQPKTVACFCSKEEQCLYNETSKEGNSSLEVTSCKCDGDTFGRLCERSKDPCDEPCFPNVNCIPGKGCEACPPNTTGDGRHCAALEDSCPNRSCPMNYCYNNGHCDISEAPGCQPTCTCPPAFTDNRCFLAGNSFTPTISMELPLRTIVLSLREDENASAADVNASVANILENLDMRAFFSNSLVELIRTSPGAQPSSKSIHHWKVTSHFKYRPRGPLIHYLNNQLIGAVMEAFLLQARQERQKRSGEARKDVHFFPISRADVQDQMALNLSMLEEYFTCDGYKGYHLVYSPQDGVTCVSPCSEGYCHNGGQCKHLPDGPQCSCASFTIYSSSGEHCEHLSVKLGAFYGILFGTLGALLLLGILAFMIFHFCGCSKNKFSYPLDSEL

Target ORF information:

RefSeq Version	NM_080457.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus mucin 4 (Muc4), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_080457.3

ORF Insert Sequence:

ATGAGAGGGC CTCACTGGAG AGTTCCCTGG CTGTGTCTGA GCTGCCTGTA TTCTTGCCTC 
CTCTTGCTAC CTGATGCTCT AGCAACAACA TCAACACAGA CACCCATGTC CCTTTCCAGC 
AGCACCAGAA CATCCCAAAT GAGCTCACAA GCATCGACCT CATCAACATC TTCTGACAGA 
AGGACAAGTA AAACAGAACA AACATCAACA AGGGATACTC CAAGTTCCAT AACAACAGTT 
TCTCAGAGCC ATCATACTAC AAGCATGGAG ACCAGCAAAC CTCAAACCAC CACAACCACC 
GAGGTGACCA CATCAACTCC TTCAGCCTCC TCACGTGACC AAATACAGAC AGAGACAAGT 
TCTCAAAGAA CAATCTCTCC TGATGGAACA ACCACCTCAC ATGCTCCCAG TATCAGCAGC 
TCAGCTCCAA GTACAACACA CATGTTAACC ACAACATCCT CCACAGAAAG TACCTCAGTA 
GACTCAGGAC ACACAACAGC AATAACAACT CAAGGTTTAA CACCTGCCAC CGCACAAGTC 
TCACTGACAC CTTCATCCCA GAATATGTCA ACAGTGTCAA CACCCATCAC CTCAACTCTT 
ACTCAGAGAC AACACACTGG AAGCAAGCAG ACCAGCAGCA AATCTCAAGT CAACATAGTC 
ACATCAACTC TTTCAACCTC TACAAGTGAC TCAACTCCTG CACAGACAAT GTCTCAAGTG 
ACATCATCTT CAGATAAAAG AACCAAGCCA TCTACTTCTG GTGTCAGCAG TACATCACTA 
ACTACAACAG AAGTGCTCAC CCAAACATCC TCTACAGACT CTGCTCCAGG AAACACAACT 
CTGAGGATTA CTCAGAACTC TACAACACAT ACCACGAAAG TCTCAACAAC ATCTACACCA 
CAAAAACTGT CACCAGTGTC AACACTCATC AATTCAAGTC AAAAAATGTC AACCCTTCCT 
CAGAACCAAC ACACTGAAAG CATGGACACC AGCAGACAAC CTCAAACCAC CACAACCATA 
GAGGTGACCA CATCAACCCC TTCAGCCTCC TCACTTCACC AAATTCAGAC AGAGACAAAT 
TCCCCAAAAA CAATCTCTCC TGGTGAGACA ACCACCTCAC ATGCTCCGAA CATGAGAAGC 
TCACCACCAA AGACATCACA GATACTAACC ACAATGCCCT CCACAAAAAG TACCTCAGTA 
GACACAAAAC AAACAAAGGC AATAACTACT AAAGTTTCTA CACCTGACAC CACACAGGTC 
TCGATGACAC CTTCATCCCA GAAATTGCCA ACACACAGCA CGTCTACTCA GGAATTAACA 
TCTTCTTACA GCCAACATAT TCAAAGCAAG GGGACCAGCA GCAAATCTCA AACAACCACA 
AACACCAAGG TGAACACATC AACTCCTTCA GCCTCCTCAC GTGACAAAAT TCAGACAGAG 
ACAAGTTCCC AAAGAACAAA CTCTCCTGGC GAGAAAAGGA CATCACATGC TCCCAGCATG 
AGCAGCTCAG CTCCAAGTAC AACACACATG TTATCCACAA CATCCTCCAA TCAAAGTACC 
TCAGTAGACA CAGGACAAAC AACATCAGTA ACAGCTCAAG GTTCTACACC TGCCATCACA 
CAGACCTCAT TGACACCTTC ATCCCAGAAT ACGTCAACAG TGTCAACACC CATCACCTCT 
ACTCACAAAT TGTCAACCCT TTCTCAGAGC CAACACACTG GAAGCAAGGG GACCAGCAGC 
AATCCTCAAA CTACCACCAC CACAGAGGTG ACCACATCAA CTCCTTCAGC CACCACCCAT 
GACCAAATTC AGACAGAGAC AAGTTCCCAA AACACAATCT CTCCTGGTGA GACAACCACC 
TCATATGCTC CCATCATGAG CAGCTCAGCT CCAAGTACAA CACATATGTT ATCCACAACA 
TCCTCCACTC AAAGTACCTC AGTAGACACA AGACACACTA CAACATTAAC GAATCAAGGA 
TCTACACCTG CCACCACACA GGTCTCACCT TCATCCCAGA ACATGTCAAC AGTGTCAGCA 
CCCATCACCT CAACTCAAAT TTTGTCAACC TTTCCTCAGA GCCAACACAC TGGAAGCAAG 
GGGACCAGCA GCAATCCTCA AACTACCACC ACCCCAGTGG TGACCACATC AAATCCTTCA 
GCCACCTCAC GTGACCAAAT TCAGACAGAG ACAAGTTCCC AAAGAACAAT CTCTCCTGGT 
GAGACAACCA CCTCATATGC TTCCATCATG AGCAGCTCAG CTCCAAGTAC AACACACATG 
TTAACCACAA CATCCTCCAC TCAAAGTACC TCAGTAGACA CAAGACACAC AATAGCAGTA 
AGGACTCAAG GTTCTACACC TGCCACCACA CAGGTCTCAC CTTCATCCCA GAACATGTCA 
ACAGTGTCAA CACCCATCAC CTCAACTCAA ATTTTGTCAA CCCTTCCTCA GAGCCAACAC 
ACTGGAAGCA AGGGGACCAG CAGCAATCCT CAAACTACCA CAAGCCCAGT GGTAACCACA 
TCAACTCCTT CAGGCACCTC AGGTGACCAA ATTCAGACAG AGACAAGTTC CCAAAGAACA 
ATCTCTCCCG GCAAGACAAC CACTTCACAT GCTCTCAACA TAAACAGCTC AGCTCCAAGT 
ACAACACACA TGTTATCCAC AACATCCTCC ACTCAAAGTA CCTCAGGAGA CACAAGACAC 
ACAACAGCAG GAAGGACTCA AGGTTCTACA CCTGCCACCA CACAGGTCTC ACCTTCATCC 
CAGAACATGT CAACAGTGTC AGCACCCATC ACCTCAATTC AAATGTTGTC AACCCTTCCT 
CAGAGCCAAC ACACTGAAAG CAAGAGTACC AGCACCAATC CTCAAACTAC CACCACCCCA 
GAGGTGACCA CATCAAATCC TTCAGCCACC TCACATGACC AAATTGAGAC AGAAACAAGT 
TCCCAAAGAA CAATCTCTCC TGGTGAGACA ACCACCTCAT ATGCTCCCAT CATGAGCAGC 
TCAGCTCCAA GTACAACACA CATGTTATCC ACAACATCCT CCACTCAAAG TACCTCAGTA 
GACACAAGAA ACACTACAAC ATTAACGACT CAAGGATCTA CACCTGCCAC CACACAGGTC 
TCACCTTCAT CCAAGAACAT GTCAACAGTG TCAACACCAA TCACCTCTAC TCACAAATTG 
TCAACCCTTC CTCAGAGCCA ACACACTGGA AGCAATGGGA CCAGCAGCTC AAGTTCTACA 
CCTGCCACAC ACAGGTCTCA CCTTCATCCC AACATGTCAA CAGTGTCAAC ACCCATCACC 
ACTACTCACA AATTGTCAAC CCTTTCTCAG AGCCAACACA CTGGAAGCAA GGGGACCAGC 
AGCAATCCTC AAACTACCAC AACGCCAGTG ATGACCACAT CAACTCCTTC AGCCACCACC 
CATGACCAAA TTCAGACAGA GACAAGTTCT CAAAGAACAA TCTCTCATGG TGAGACAACC 
ACCTCATATG CTCCCATCAT GAGCAGCTCA GCTCCAAGTA CAACACACAT GTTATCAACA 
GCATCCTCTA CTCAAATTAC CTCAGTAGAC ACAAGACACA CAACAGCAAT AACAACTCAA 
GGTTCTACAC CTGCCACCAC ACAGGTCTCA CCTTCATCCC AGAACATGTC AACAGTGTCA 
GCACCCATCA CCTCAATTCA AATTTTGTCA ACCCTTCCTC AGAGCCAACA CACTGGAAGC 
AAGGGGACCA GCACCAATCC TCAAACTACC ACCACCCCAG AGGTGACCAC ATCAACTCCT 
TCAGCCACCT CACGTGACCA AATTCAGACA GAGACAAGTT CCCAAAGAAC AATCTCTCCT 
GGTGAGACAA CCACCTCACA TGCTCCCATC ATGAGCAGCT CAGCTCCAAG TACAACACAC 
ATGTTATCCA CAACATCCTC CACTCAAAGT ACCTCAGTAG ACACAAGACA CACAACAGCA 
GGAAGGACTC AAGGTTCTAC ACCTGCCACC ACACAGGTCT CACCTTCCTC CCAGAACATG 
ACAACCACCT CACATGCCCT CATGAGCAGC TCAGCTCCAA GTACAACACA CATGTTATCC 
ACAACATCTT CCACTCAAAG TACCTCAGTA GATACAAGAC ACACTACAAC AGTAACGACT 
CAAGGTTCTA CACCTGCCAC CACACAGGTC TTACCTTCAT CCCAGAACAT GTCAACAGTG 
TCAGCTCCCA TCACCTCAAC TCAAATTTTG TCAACCCTTC CTCAGAGCCA ACACACTGGA 
AGCAAGGGGA CCAGCACCAA TCCTCAAACT ACCACCAATG CAGAGGTGAC CACATCAACT 
CCTTCAGCCA CCTCACATGA CCAAATTGAG ACAGAAACAA GTTCCCAAAG AACAATCTCT 
CCTGGTGAGA CAACCACCTC ACATGCTCCC AACATGAGTA GTTCAGCTCC AAGTACAACA 
CACATGTTAT CCTCAACATC CTCCACTCAA ATTACCTCAG TAGACACAGG ACACACATCA 
GCAGGAAGGA CTCAAGGTTC TACACCTGCC ACCACACAGG TCTCACCTTC ATCCCAGAAC 
ATGTCAACAG TGTCAGCACC CATCACCTCA ACTCATATTT TGTCAACCCT TCCAAAGAGC 
CAACACACTG GAAGCAAGGG GACCAGCAGC AATCCTCAAA CTACCATAAC CCCAGTGGTA 
ACCACATCAA CTCCTTCAGC CTCCTCCCGT GACCAAATTC AGACAGAGAC TAGTTTCCAA 
AGAACAATCT CTCCTGGCGA GACAACCACC TCACATGCTC CCAGCATGAG CAGCTCAGCT 
CCAAGTTCAA CACACATGTT ATCAACAGCA TCCTCTACTC AAATTACCTC AGTAGACACA 
AGACACACAA CAGCAATAAC AACTCAAGGT TCTACACCTG CCACCACACA GGTCTCACCT 
TCATCCCAGA ACATGTCAAC AGTGTCAGCA CCCATCACCT CAATTCAAAT GTTGTCAACC 
CTTCCTCAGA GCCAACACAC TGAAAGCAAG AGTACCAGCA CCAATCCTCA AACTACCACC 
ACCCCCAGAG TGACCACATC AACTCCTTCA GCCTCCTCAC GTGACCAAAT TCAGACAGAG 
ACAAGTTCCC AAAGAACAAT CTCTCCCGGC AAGACAACCA CCTCACATGT TCCCAACATG 
AACAGCTCAG CTCCAAGTAC AACACACATC TTATCCACAA CATCCTCCAT TCAAAGTACC 
TCAGGAGATA CAAGACACAC AACAGCAGTA AGGACTCAAG GTTCTACACC TGCCACCACA 
CAGGTCTCAC TGGCACCTTC ATCCCAGAAC ATGTCAACAC TGTCAGCACC CATCACATCA 
CCTCAACATT TTTCAACCCT TCCTCAGAAC CAACACACTG GAAGCATGGG GACCAGCAGC 
AATCCTCAAA GTACCACCAT CCCAGAGGTG ACCACATCAA CTCTTTCAGC TTCCTCACGT 
GACCAAGTTC AGACAGAGAC AAGTTCCCAA AGAACAATCT CTCCTGGTGA GACAACCACC 
TCACATGCTT CCAGCTTGAG CAGTTCAGCT CCAAGTTCAA CACACATGTT ATCCACAGCA 
TCCTCCACTG AAATTACCTC AGGAGACACA AGACACACAA CAGCAATAGT GACTCAAGGT 
TCTACACCTG CCACCACACA GACCACATTG ACACCTTCGT CCCAGAATAT GTCAACAGTG 
TCAACACCCA TCACTTCTAC TCACAAATTG TCACCCCTTC CTCAGAGCCA ACACACTGAA 
AACATGGGGA CCAGCAGCAA TCCTCAAACT ACCACCACCC CAGAAGTGAC CACATCAACT 
CCTTCAGCCA CCTCCTATGA CCAAATTCAG ACAGAGACAA GTTTCCAAAG AACAATCTCT 
CCTGGTGAGA CAACCACTTC ACATGCTCCC AGCATGAGCA ACTCAGCTCC AAGTTCAACA 
CACAAGTTAT CAACAGCATC CTCCACTGAA ATTACCTCAG TAGACACAAG ACACACAATA 
GCAATAACGA CTCAAGGTTC TACACTTGCC ACCACACAGA CCTCACTGAC ACCTTCATCC 
CAGAACATGT CAACAGTGTC AGCACCCATC ACCTCATCTC AAATTTTGTC AACTCTTCGT 
CAGAGCCAAC ACACTGGAAG CAAGGGGACC AGCAGCAATC ATCAGACTAC CACTACCCCT 
GTGGTGACCA CATCAACTTC TTCAGCCACC TCACGTGACC AAATTCAGAC AGAGACAAGT 
TCCCTAAGAA CAATCTCTCC TGATGGAACG ACCACCTCAC ATGCTTCCAG CATGAGCAGT 
TCATCTCCAA ATACAACACA CTTGTTAATC ACTACATCCT CCACAGAAAG TACCTCAGTA 
GACACAGGAT ACTCAACAGT AATAACGACT CATGGTTCTA CACTTGCCAC CACCCAGGTC 
TCATTGACAC CTTCATCCCA GAATATGTCA ACAGTGTCAA TGCCCAGCAC TTCTTCACAG 
GAATTAACAT CACTTCCTCA GAGACAACAC ACTGGAAACA TGGAGACCAG CAGCCAACCT 
CAGAATATCA CCCCTACTGT GGTGACCACA TCAACTCTTT TATCATTCTC AAGGGGATCC 
ACTGAATTGC AAACCATGTC TTGGGGAACG TCCTCCTCAG GGACAATAAA TACCCTATCT 
ACTCCCGTCA GGAATACCTC ACCTGCATCC ACATCAGGCA TCCTCACCAG CACATTGACC 
ACCTCAGGAA ACACAGGGTA CACAGGTGTA ACTAGAAGCC TCGGTGTGAT CACATCAAGA 
GTCACAAGCA CAAGTCTACC TGGAAAGTCC ACAGTAGTGC ATAGCACCCC TGCTCAGCCA 
TTGTCTGCAC ATTCTCAGAG TCACCAGACG TATGGCACGG GGACACCCAG CATGTCTCAA 
ACCAGCATCC TCCCAGACAT GACCTCAGAA AAGCGTGTTG CCTCTTCACC TGGACCCACT 
GTGACAGAGT CCTTTTCTCA TGTGTCATCC TCCTCAGGAT TGACTACGAA GACAGACAAT 
GACAGAAACA CAGCAGTCTC AGCCACCTCC TCGACCCTTA CTAGCCCCAG CCCAACCACA 
GCCTCCAGGT CAACAGTGCC CCTGCCTTCT CTTTTACCAG ATCAGGGCAT TTCCCTCTTC 
CCCTACGGGT CTGAAGTCGG AGACCAGAAT TTGTTTGCCA GGACTGTGGA TTTTAACTCA 
CCAATCTTCA AGATCCTGAT TGGCTTTCCC CTCGGTTCCT CCCTGCGGGA TTCCTTCTAC 
GTTACAGATA ATGGTCAGAT CATTTTCCCA GAGTCAGACT ATGACGTCTT CTCCTACCCC 
AACCCACCTC AAAGAGGCTT CACCGGAAGG GAGCGCGTGG CCATGGTGGC CCCGTTCTGG 
GCTGATGCTG ATTTCTCTAG CTCCAGGGGA GCCATATTTT ACCAGGAATA TGTCACATTT 
TACAATGAAC ACCATCAGCT AATCCGGGAA GTGGAGACTT TGATTAATGA TTTCACAAGT 
AGCTGGGGCT ACAGAGCCAA GTGGACACTG AAGGTCACCT GGGTCAATGT TCCTGCCTAT 
ACTGCCCAAG AGAGCTTTGG GACCAATACC TACCAAGCCA TCCTCTCCAC AGATGGGAGC 
AGGTCCTATG CCCTGTTTCT CTACCAGAAC AGTGGCATGA GGTGGGATGT GACCCAGGAA 
CCATACAACC GGGTGCTCAT GGGCTTCTCC AGTGGAGATG GGTATTTTGA AAACAGCCCG 
CTGACATTCC GGCCAGCAAT GGAGAAGTAC CGTCCAGATC GATTCCTGAA TTCCAAATTA 
GGCATCCGGG GACTGCAGGT CTACAGGCTG CACAGGGAGG AGCGGCCCAA CTACCGGCTC 
AAATGTCTGC AGTGGCTGGA GAGTCAGCCT CAGCAGCCCA GCTGGGGCTG GAGCTCAGTT 
TCCTGCCCTT GCTCCTGGCA GCAGGGACAA CGGGACTTTC GGTTCAGGCC CATCAACCCA 
GGTTGGTGGG ACAGGCAGCT GTGCAGCTTC TCCTCCGGGC GAGGGGGTGT GTGCTGCAGC 
TATGGTGCCT GGGGAGAGTT TCGAGAAGGC TGGAGAATGC ACAGTCCTTG GCAGTTTGAT 
GAGGAGCAGG AGGCACAGAA CTGGTGCTGC CAATGGAACG ACAAGCCCTC GTTCTGTGTC 
TGGTACCAGC TTAGGCGGCC CCGTGTCAGC TGTGCTGGGT ACAGGCCCCC ACGGCCCGCT 
TGGACATTTG GTGATCCCCA CATCACCACT TTGGATAATG CCAACTTCAC CTTCAACGGG 
CTGGGAGACT TTCTGCTGGT CCAGGCCCAG GACAGAAATT CTTCCTTCCT GCTGGAAGGC 
CGCACTGCCC AGACTGGCAC TGCCAAGGCC ACTAACTTCA TTGCCTTTGC GGCTCAATAC 
AACACCAGCA GCCTGAAGTC TCCCATCACG GTTCAGTGGT TTCTTGAGCC CAGTGACAAA 
ATCCGAGTCG TATACAATAA CCAGACTGTG GCCTTTAACA CCAGAGACAC TGAAGTCTTA 
CCCATATTCA ATACCACCGG TGTTCTGCTG ACCCAAAATG GCTCCCAAGT CTCAGCCAAC 
TTTGATGGGA CAGTGACTAT CTCTGTGATT GCTCGCTCCA ACATCCTTCA TGCCTCCTCC 
AGCCTATCAG AGGAATATCG AAACCACACA GAGGGCCTTC TGGGAGTGTG GAATGACAAT 
CCAGAAGATG ACTTCAGGAT GCCCAATGGC TCCACCATTC CCTCAAACAG CTCTGAGGAG 
ACCCTTTTCT ACTATGGAAT GACATGGCAC GTCAACGGAA CAGGCCTCCT TGGGATAAGG 
GCAGACCCGC TGCCTACCAA GTTCACCCCC ATCTTTCTGT CTCAACTGTT GAATCAGAGT 
GCCTCAGGTG AAGACTTGGC CTCTGGGTGC AAAGGCGACA GAAAGTGCAT GTTTGACATC 
CTGGCCACGG GAAACAGAAC TATTGGACAA AGCACCAATT CCATCCTTAA TGAGTTCCAA 
CATATGAATG ACACCCTCAA TCAGTACCCA CCCTCTATCA ACTGCAGCAG CAAGATTCAA 
GCCTACAAGG GGCAGACAGT GACCACTGAG ATCACCAGCA ACTCTAAGGA TGCCACACTC 
AGCCTCTCCA AGAAATGTAG TGGCTTTAAG CTCTTTGAAA ACGGGAGTTT GCAGTGGACA 
CCAACATCCC CAGAAGCGTG TACCCTGGAG ATTCTAGCGA GGGATGTCAG GACTAACTTG 
TCATGGGTAC TCCAGCCCAA GACCGTGGCT TGCTTCTGCA GTAAGGAGGA GCAGTGTTTG 
TACAATGAAA CCAGCAAAGA GGGCAATTCT TCCCTTGAGG TGACCAGCTG CAAGTGTGAT 
GGGGACACCT TTGGCCGCTT GTGTGAACGC TCCAAGGACC CGTGTGATGA GCCATGCTTC 
CCTAACGTGA ACTGCATTCC TGGGAAGGGC TGTGAGGCCT GCCCTCCAAA CACAACTGGA 
GATGGACGTC ATTGTGCAGC TCTGGAGGAC TCCTGCCCAA ACCGTTCCTG TCCCATGAAT 
TACTGCTATA ACAATGGCCA TTGCGACATC TCTGAGGCTC CAGGCTGCCA GCCCACTTGC 
ACCTGTCCCC CTGCCTTCAC TGATAACCGC TGCTTCCTGG CTGGGAACAG TTTCACTCCC 
ACCATCTCTA TGGAGCTTCC CTTAAGGACC ATCGTGCTCT CTCTCAGGGA GGATGAAAAT 
GCCTCCGCCG CTGACGTCAA CGCCTCGGTG GCGAACATAC TAGAGAACCT GGACATGCGG 
GCTTTTTTCT CCAACAGCTT AGTGGAACTG ATACGAACCT CTCCCGGAGC ACAGCCCTCG 
AGCAAGTCCA TTCATCACTG GAAGGTCACC TCCCACTTCA AGTACCGTCC CAGGGGCCCG 
CTCATCCACT ATCTGAACAA CCAGCTGATA GGCGCCGTGA TGGAGGCCTT CCTCCTGCAG 
GCTCGGCAGG AGAGGCAGAA GAGGAGTGGA GAAGCCAGGA AGGACGTCCA CTTCTTCCCC 
ATCTCGAGGG CAGATGTCCA GGACCAGATG GCTCTGAACC TAAGTATGCT GGAGGAGTAC 
TTCACATGTG ATGGCTACAA AGGCTACCAC CTGGTCTACA GCCCACAGGA TGGTGTCACC 
TGTGTGTCCC CATGTAGTGA GGGCTACTGT CACAATGGAG GCCAATGCAA GCACCTGCCA 
GATGGGCCCC AATGCAGCTG CGCATCCTTC ACCATCTACT CATCCTCGGG CGAACACTGT 
GAGCACCTAA GTGTGAAACT TGGGGCATTC TACGGGATCC TCTTCGGAAC CCTGGGCGCC 
CTCTTGCTAC TGGGGATCTT AGCATTTATG ATCTTTCACT TCTGCGGCTG CTCCAAGAAC 
AAGTTCTCCT ACCCTTTGGA CTCAGAATTG TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Interferon Regulatory Factor 6 Is Necessary for Salivary Glands and Pancreas Development.
Journal of dental research97(2)226-236(2018 Feb)
Metwalli KA,Do MA,Nguyen K,Mallick S,Kin K,Farokhnia N,Jun G,Fakhouri WD

Membrane Mucin Muc4 promotes blood cell association with tumor cells and mediates efficient metastasis in a mouse model of breast cancer.
Oncogene37(2)197-207(2018 Jan)
Rowson-Hodel AR,Wald JH,Hatakeyama J,O'Neal WK,Stonebraker JR,VanderVorst K,Saldana MJ,Borowsky AD,Sweeney C,Carraway KL 3rd

Quantitative proteomic profiling of the extracellular matrix of pancreatic islets during the angiogenic switch and insulinoma progression.
Scientific reports740495(2017 Jan)
Naba A,Clauser KR,Mani DR,Carr SA,Hynes RO

APC haploinsufficiency coupled with p53 loss sufficiently induces mucinous cystic neoplasms and invasive pancreatic carcinoma in mice.
Oncogene35(17)2223-34(2016 04)
Kuo TL,Weng CC,Kuo KK,Chen CY,Wu DC,Hung WC,Cheng KH

The mucin MUC4 is a transcriptional and post-transcriptional target of K-ras oncogene in pancreatic cancer. Implication of MAPK/AP-1, NF-?B and RalB signaling pathways.
Biochimica et biophysica acta1849(12)1375-84(2015 Dec)
Vasseur R,Skrypek N,Duch?ne B,Renaud F,Mart?nez-Maqueda D,Vincent A,Porchet N,Van Seuningen I,Jonckheere N

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Muc4 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

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