Epo cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Epo
Entrez Gene ID	13856
Full Name	erythropoietin
General protein information	Preferred Names erythropoietin Names erythropoietin
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 5 Map Location: 5 G2\|5 76.5 cM
Summary	This gene encodes the glycoprotein hormone erythropoietin that regulates the production of red blood cells and biosynthesis of hemoglobin. The predominant expression of this gene shifts from the liver during fetal development to kidney in adults. A complete lack of the encoded protein causes embryonic lethal anemia in mice. The conditional inactivation of this gene in adult mice results in a chronic, normocytic and normochromic anemia. Transgenic mice expressing the human ortholog of this gene exhibit polycythemia. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Aug 2015].

mRNA and Protein(s)

mRNA	Protein	Name
NM_001312875.1	NP_001299804.1	erythropoietin isoform 2 precursor
NM_007942.2	NP_031968.1	erythropoietin isoform 1 precursor

Pathways from BioSystems

Homologies

	EPO	NP_001036201.1
Danio rerio (zebrafish)	epo	NP_001033098.1
Danio rerio (zebrafish)	LOC100002479	XP_001342254.1
Bos taurus (cattle)	EPO	NP_776334.1
Pan troglodytes (chimpanzee)	EPO	XP_519268.2
Mus musculus (house mouse)	Epo	NP_031968.1
Xenopus tropicalis (tropical clawed frog)	epo	NP_001233194.1
Homo sapiens (human)	EPO	NP_000790.2
Canis lupus familiaris (dog)	EPO	NP_001006647.1
Rattus norvegicus (Norway rat)	Epo	NP_058697.1

Gene Ontology
Bibliography

Related articles in PubMed

Murine erythropoietin gene: cloning, expression, and human gene homology.
Shoemaker CB, Mitsock LD
Molecular and cellular biology6(3)849-58(1986 Mar)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

these data provide strong evidence for a role for G-CSF in the development of ACI after burn injury through suppression of EPO signaling in bone marrow erythroid cells.
Title: Thermal injury of the skin induces G-CSF-dependent attenuation of EPO-mediated STAT signaling and erythroid differentiation arrest in mice.

these findings define a cis-regulatory enhancer network for Epo signaling during erythropoiesis, and provide the framework for future studies involving the interplay of epigenetics and Epo signaling.
Title: Epo reprograms the epigenome of erythroid cells.

Smad1 and Smad5 have overlapping functions to govern hepcidin transcription. Moreover, erythropoietin and erythroferrone target Smad1/5 signaling and require Smad1/5 to suppress hepcidin expression.
Title: Smad1/5 is required for erythropoietin-mediated suppression of hepcidin in mice.

Data indicated the possible involvement of Jak2/STAT3/STAT6 pathway in the augmentation of EPO on M2 polarization. These results improved the understanding of the immunoregulatory capacity of EPO on macrophages, which might optimize the therapeutic modalities of EPO.
Title: Erythropoietin protects against rhabdomyolysis-induced acute kidney injury by modulating macrophage polarization.

The lack of effect of erythropoietin on hepcidin expression in mask mice can not be explained by changes in erythroferrone synthesis, as splenic erythroferrone content increased after erythropoietin administration in both C57BL/6 and mask mice.
Title: Effect of erythropoietin administration on proteins participating in iron homeostasis in Tmprss6-mutated mask mice.

The following Epo gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Epo cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu67663	NM_001312875.1 Latest version!	Mus musculus erythropoietin (Epo), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00
OMu18125	NM_007942.2 Latest version!	Mus musculus erythropoietin (Epo), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$69.30 $99.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Epo ( NM_001312875.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001299804.1 Mus musculus erythropoietin (Epo), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OMu67663
Clone ID Related Accession (Same CDS sequence)	NM_001312875.1
Accession Version	NM_001312875.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 576bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-10
Organism	Mus musculus(house mouse)
Product	erythropoietin isoform 2 precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BC119265.1. Transcript Variant: This variant (2) uses an alternate in-frame splice site in the 3' coding region, compared to variant 1. The encoded protein (isoform 2) is shorter than isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC144887.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164140 [ECO:0000348] ##Evidence-Data-END##

ATGGGGGTGC CCGAACGTCC CACCCTGCTG CTTTTACTCT CCTTGCTACT GATTCCTCTG 
GGCCTCCCAG TCCTCTGTGC TCCCCCACGC CTCATCTGCG ACAGTCGAGT TCTGGAGAGG 
TACATCTTAG AGGCCAAGGA GGCAGAAAAT GTCACGATGG GTTGTGCAGA AGGTCCCAGA 
CTGAGTGAAA ATATTACAGT CCCAGATACC AAAGTCAACT TCTATGCTTG GAAAAGAATG 
GAGGTGGAAG AACAGGCCAT AGAAGTTTGG CAAGGCCTGT CCCTGCTCTC AGAAGCCATC 
CTGCAGGCCC AGGCCCTGCT AGCCAATTCC TCCCAGCCAC CAGAGACCCT TCAGCTTCAT 
ATAGACAAAG CCATCAGTGG TCTACGTAGC CTCACTTCAC TGCTTCGGGT ACTGGGAGCT 
CAGGAATTGA TGTCGCCTCC AGATACCACC CCACCTGCTC CACTCCGAAC ACTCACAGTG 
GATACTTTCT GCAAGCTCTT CCGGGTCTAC GCCAACTTCC TCCGGGGGAA ACTGAAGCTG 
TACACGGGAG AGGTCTGCAG GAGAGGGGAC AGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001299804.1
CDS	72..647
Translation	MGVPERPTLLLLLSLLLIPLGLPVLCAPPRLICDSRVLERYILEAKEAENVTMGCAEGPRLSENITVPDTKVNFYAWKRMEVEEQAIEVWQGLSLLSEAILQAQALLANSSQPPETLQLHIDKAISGLRSLTSLLRVLGAQELMSPPDTTPPAPLRTLTVDTFCKLFRVYANFLRGKLKLYTGEVCRRGDR

Target ORF information:

RefSeq Version	NM_001312875.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus erythropoietin (Epo), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001312875.1

ORF Insert Sequence:

ATGGGGGTGC CCGAACGTCC CACCCTGCTG CTTTTACTCT CCTTGCTACT GATTCCTCTG 
GGCCTCCCAG TCCTCTGTGC TCCCCCACGC CTCATCTGCG ACAGTCGAGT TCTGGAGAGG 
TACATCTTAG AGGCCAAGGA GGCAGAAAAT GTCACGATGG GTTGTGCAGA AGGTCCCAGA 
CTGAGTGAAA ATATTACAGT CCCAGATACC AAAGTCAACT TCTATGCTTG GAAAAGAATG 
GAGGTGGAAG AACAGGCCAT AGAAGTTTGG CAAGGCCTGT CCCTGCTCTC AGAAGCCATC 
CTGCAGGCCC AGGCCCTGCT AGCCAATTCC TCCCAGCCAC CAGAGACCCT TCAGCTTCAT 
ATAGACAAAG CCATCAGTGG TCTACGTAGC CTCACTTCAC TGCTTCGGGT ACTGGGAGCT 
CAGGAATTGA TGTCGCCTCC AGATACCACC CCACCTGCTC CACTCCGAAC ACTCACAGTG 
GATACTTTCT GCAAGCTCTT CCGGGTCTAC GCCAACTTCC TCCGGGGGAA ACTGAAGCTG 
TACACGGGAG AGGTCTGCAG GAGAGGGGAC AGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Epo ( NM_007942.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_031968.1 Mus musculus erythropoietin (Epo), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OMu18125
Clone ID Related Accession (Same CDS sequence)	NM_007942.2
Accession Version	NM_007942.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 579bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-10
Organism	Mus musculus(house mouse)
Product	erythropoietin isoform 1 precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BC119265.1. On Sep 3, 2006 this sequence version replaced NM_007942.1. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer protein (isoform 1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC119265.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164135, SAMN01164137 [ECO:0000348] ##Evidence-Data-END##

ATGGGGGTGC CCGAACGTCC CACCCTGCTG CTTTTACTCT CCTTGCTACT GATTCCTCTG 
GGCCTCCCAG TCCTCTGTGC TCCCCCACGC CTCATCTGCG ACAGTCGAGT TCTGGAGAGG 
TACATCTTAG AGGCCAAGGA GGCAGAAAAT GTCACGATGG GTTGTGCAGA AGGTCCCAGA 
CTGAGTGAAA ATATTACAGT CCCAGATACC AAAGTCAACT TCTATGCTTG GAAAAGAATG 
GAGGTGGAAG AACAGGCCAT AGAAGTTTGG CAAGGCCTGT CCCTGCTCTC AGAAGCCATC 
CTGCAGGCCC AGGCCCTGCT AGCCAATTCC TCCCAGCCAC CAGAGACCCT TCAGCTTCAT 
ATAGACAAAG CCATCAGTGG TCTACGTAGC CTCACTTCAC TGCTTCGGGT ACTGGGAGCT 
CAGAAGGAAT TGATGTCGCC TCCAGATACC ACCCCACCTG CTCCACTCCG AACACTCACA 
GTGGATACTT TCTGCAAGCT CTTCCGGGTC TACGCCAACT TCCTCCGGGG GAAACTGAAG 
CTGTACACGG GAGAGGTCTG CAGGAGAGGG GACAGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_031968.1
CDS	72..650
Translation	MGVPERPTLLLLLSLLLIPLGLPVLCAPPRLICDSRVLERYILEAKEAENVTMGCAEGPRLSENITVPDTKVNFYAWKRMEVEEQAIEVWQGLSLLSEAILQAQALLANSSQPPETLQLHIDKAISGLRSLTSLLRVLGAQKELMSPPDTTPPAPLRTLTVDTFCKLFRVYANFLRGKLKLYTGEVCRRGDR

Target ORF information:

RefSeq Version	NM_007942.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus erythropoietin (Epo), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_007942.2

ORF Insert Sequence:

ATGGGGGTGC CCGAACGTCC CACCCTGCTG CTTTTACTCT CCTTGCTACT GATTCCTCTG 
GGCCTCCCAG TCCTCTGTGC TCCCCCACGC CTCATCTGCG ACAGTCGAGT TCTGGAGAGG 
TACATCTTAG AGGCCAAGGA GGCAGAAAAT GTCACGATGG GTTGTGCAGA AGGTCCCAGA 
CTGAGTGAAA ATATTACAGT CCCAGATACC AAAGTCAACT TCTATGCTTG GAAAAGAATG 
GAGGTGGAAG AACAGGCCAT AGAAGTTTGG CAAGGCCTGT CCCTGCTCTC AGAAGCCATC 
CTGCAGGCCC AGGCCCTGCT AGCCAATTCC TCCCAGCCAC CAGAGACCCT TCAGCTTCAT 
ATAGACAAAG CCATCAGTGG TCTACGTAGC CTCACTTCAC TGCTTCGGGT ACTGGGAGCT 
CAGAAGGAAT TGATGTCGCC TCCAGATACC ACCCCACCTG CTCCACTCCG AACACTCACA 
GTGGATACTT TCTGCAAGCT CTTCCGGGTC TACGCCAACT TCCTCCGGGG GAAACTGAAG 
CTGTACACGG GAGAGGTCTG CAGGAGAGGG GACAGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Murine erythropoietin gene: cloning, expression, and human gene homology.
Molecular and cellular biology6(3)849-58(1986 Mar)
Shoemaker CB,Mitsock LD

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Epo cDNA ORF clone, Mus musculus(house mouse)

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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