En2 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	En2
Entrez Gene ID	13799
Full Name	engrailed 2
Synonyms	BB131122,En-2
General protein information	Preferred Names engrailed 2 Names homeobox protein engrailed-2 homeobox protein en-2 mo-En-2
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 5 Map Location: 5 B1\|5 13.94 cM

mRNA and Protein(s)

mRNA	Protein	Name
NM_010134.3	NP_034264.1	homeobox protein engrailed-2
NM_010134.4	NP_034264.1	homeobox protein engrailed-2

Pathways from BioSystems

Homologies

Gallus gallus (chicken)	LOC100859435	NP_001254648.1
Danio rerio (zebrafish)	eng2a	NP_571119.2
Xenopus tropicalis (tropical clawed frog)	LOC100498251	XP_002932525.1
Pan troglodytes (chimpanzee)	EN2	XP_001146034.2
Mus musculus (house mouse)	En2	NP_034264.1
Danio rerio (zebrafish)	eng2b	NP_571115.1
Homo sapiens (human)	EN2	NP_001418.2
	EN2	XP_001106130.1
Rattus norvegicus (Norway rat)	En2	NP_001102684.1

Gene Ontology
Bibliography

Related articles in PubMed

Higher-Order Kidney Organogenesis from Pluripotent Stem Cells.
Taguchi A, Nishinakamura R
Cell stem cell21(6)730-746.e6(2017 Dec)

Increased signaling by the autism-related Engrailed-2 protein enhances dendritic branching and spine density, alters synaptic structural matching, and exaggerates protein synthesis.
Soltani A, Lebrun S, Carpentier G, Zunino G, Chantepie S, Ma?za A, Bozzi Y, Desnos C, Darchen F, Stettler O
PloS one12(8)e0181350(2017)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

This study demonstrated that Ketogenic diet exposure during the juvenile period increases social behaviors and forebrain neural activation in adult Engrailed 2 null mice.
Title: Ketogenic diet exposure during the juvenile period increases social behaviors and forebrain neural activation in adult Engrailed 2 null mice.

Study showed that BDNF signaling through its low-affinity receptor p75 is compromised in the forebrain of En2-/- mice; BDNF signaling deficits might be involved in the neurodevelopmental alterations observed in the En2-/- forebrain
Title: Brain-derived neurotrophic factor signaling is altered in the forebrain of Engrailed-2 knockout mice.

The homeoprotein Engrailed is not only a survival factor for mesencephalic dopaminergic (mDA) neurons during development, but continues to exert neuroprotective and physiological functions in adult mDA neurons. [Review]
Title: Dissecting the role of Engrailed in adult dopaminergic neurons--Insights into Parkinson disease pathogenesis.

En2 deletion reduces monoamine innervation of target forebrain structures, affecting forebrain structural growth, postnatal neurogenesis and related behavior.
Title: Engrailed-2 (En2) deletion produces multiple neurodevelopmental defects in monoamine systems, forebrain structures and neurogenesis and behavior.

confirm the early neurotransmitter deficit with a late compensatory recovery in En2 KO-mice further suggesting that this experimental animal may be considered a good predictive model for the human disease
Title: Serotonin abnormalities in Engrailed-2 knockout mice: New insight relevant for a model of Autism Spectrum Disorder.

The following En2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the En2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu05601	NM_010134.4 Latest version!	Mus musculus engrailed 2 (En2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$188.30 $269.00
View Old Accession Versions >>
OMu05601	NM_010134.3	Mus musculus engrailed 2 (En2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$188.30 $269.00
Hide Old Accession Versions >>

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

En2 ( NM_010134.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_034264.1 Mus musculus engrailed 2 (En2), mRNA.

Price & Availability↑

CloneID	OMu05601
Clone ID Related Accession (Same CDS sequence)	NM_010134.3 , NM_010134.4
Accession Version	NM_010134.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 975bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-29
Organism	Mus musculus(house mouse)
Product	homeobox protein engrailed-2
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AK157766.1. On Apr 6, 2007 this sequence version replaced NM_010134.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK157766.1, L12705.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849376, SAMN00849379 [ECO:0000348] ##Evidence-Data-END##

ATGGAGGAGA AGGATTCCAA GCCCAGCGAG ACGGCGGCGG AGGCGCAGAG ACAGCCGGAA 
CCCAGCTCCG GCGGCGGCTC TGGCGGCGGC AGCAGCCCGA GCGACTCGGA CACCGGCCGC 
CGGCGGGCTC TGATGCTGCC CGAGGTCCTA CAGGCGCCAG GCAACCACCA GCATCCACAT 
CGCATCACCA ACTTCTTCAT CGATAACATC CTGCGGCCTG AGTTTGGCCG CCGAAAGGAC 
GCGGGGACTT GCTGTGCGGG CGCGGGCGGA GCCAGGGGAG GCGAAGGCGG CGCTGGCACT 
ACCGAAGGAG GCGGCGGCGG CGCAGGCGGA GCCGAGCAGC TACTGGGCGC CAGGGAGTCC 
CGACCGAACC CAGCGTGCGC ACCCAGCGCG GGAGGAACGC TCTCCGCCGC CGCTGGCGAC 
CCTGCGGTCG ACGGAGAAGG AGGTTCCAAG ACGCTATCAC TTCACGGTGG TGCCAAAAAA 
CCCGGCGATC CTGGGGGTTC CTTGGATGGA GTGCTCAAAG CCCGGGGCTT GGGCGGCGGT 
GACCTGTCGG TGAGCTCCGA CTCGGACAGC TCTCAAGCCA GCGCCACTCT GGGCGCGCAG 
CCCATGCTCT GGCCCGCTTG GGTCTACTGC ACGCGCTATT CTGACCGGCC TTCTTCAGGT 
CCCAGGTCCC GAAAACCAAA GAAGAAGAAC CCTAACAAAG AGGACAAGCG GCCTCGCACA 
GCCTTCACTG CTGAGCAGCT CCAGAGGCTC AAGGCTGAGT TTCAGACCAA CAGGTACCTG 
ACAGAGCAGC GGCGCCAGAG TCTGGCACAG GAGCTCAGCC TGAACGAGTC TCAGATCAAG 
ATTTGGTTCC AGAACAAGCG GGCCAAAATC AAGAAAGCCA CGGGCAACAA GAACACTTTG 
GCGGTGCACC TCATGGCACA GGGCCTGTAC AACCATTCCA CCACGGCCAA GGAGGGCAAG 
TCGGACAGCG AGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_034264.1
CDS	832..1806
Translation	MEEKDSKPSETAAEAQRQPEPSSGGGSGGGSSPSDSDTGRRRALMLPEVLQAPGNHQHPHRITNFFIDNILRPEFGRRKDAGTCCAGAGGARGGEGGAGTTEGGGGGAGGAEQLLGARESRPNPACAPSAGGTLSAAAGDPAVDGEGGSKTLSLHGGAKKPGDPGGSLDGVLKARGLGGGDLSVSSDSDSSQASATLGAQPMLWPAWVYCTRYSDRPSSGPRSRKPKKKNPNKEDKRPRTAFTAEQLQRLKAEFQTNRYLTEQRRQSLAQELSLNESQIKIWFQNKRAKIKKATGNKNTLAVHLMAQGLYNHSTTAKEGKSDSE

Target ORF information:

RefSeq Version	NM_010134.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus engrailed 2 (En2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_010134.3

ORF Insert Sequence:

ATGGAGGAGA AGGATTCCAA GCCCAGCGAG ACGGCGGCGG AGGCGCAGAG ACAGCCGGAA 
CCCAGCTCCG GCGGCGGCTC TGGCGGCGGC AGCAGCCCGA GCGACTCGGA CACCGGCCGC 
CGGCGGGCTC TGATGCTGCC CGAGGTCCTA CAGGCGCCAG GCAACCACCA GCATCCACAT 
CGCATCACCA ACTTCTTCAT CGATAACATC CTGCGGCCTG AGTTTGGCCG CCGAAAGGAC 
GCGGGGACTT GCTGTGCGGG CGCGGGCGGA GCCAGGGGAG GCGAAGGCGG CGCTGGCACT 
ACCGAAGGAG GCGGCGGCGG CGCAGGCGGA GCCGAGCAGC TACTGGGCGC CAGGGAGTCC 
CGACCGAACC CAGCGTGCGC ACCCAGCGCG GGAGGAACGC TCTCCGCCGC CGCTGGCGAC 
CCTGCGGTCG ACGGAGAAGG AGGTTCCAAG ACGCTATCAC TTCACGGTGG TGCCAAAAAA 
CCCGGCGATC CTGGGGGTTC CTTGGATGGA GTGCTCAAAG CCCGGGGCTT GGGCGGCGGT 
GACCTGTCGG TGAGCTCCGA CTCGGACAGC TCTCAAGCCA GCGCCACTCT GGGCGCGCAG 
CCCATGCTCT GGCCCGCTTG GGTCTACTGC ACGCGCTATT CTGACCGGCC TTCTTCAGGT 
CCCAGGTCCC GAAAACCAAA GAAGAAGAAC CCTAACAAAG AGGACAAGCG GCCTCGCACA 
GCCTTCACTG CTGAGCAGCT CCAGAGGCTC AAGGCTGAGT TTCAGACCAA CAGGTACCTG 
ACAGAGCAGC GGCGCCAGAG TCTGGCACAG GAGCTCAGCC TGAACGAGTC TCAGATCAAG 
ATTTGGTTCC AGAACAAGCG GGCCAAAATC AAGAAAGCCA CGGGCAACAA GAACACTTTG 
GCGGTGCACC TCATGGCACA GGGCCTGTAC AACCATTCCA CCACGGCCAA GGAGGGCAAG 
TCGGACAGCG AGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

En2 ( NM_010134.4 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_034264.1 Mus musculus engrailed 2 (En2), mRNA.

Price & Availability↑

CloneID	OMu05601
Clone ID Related Accession (Same CDS sequence)	NM_010134.3 , NM_010134.4
Accession Version	NM_010134.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 975bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2020-01-04
Organism	Mus musculus(house mouse)
Product	homeobox protein engrailed-2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK157766.1 and AI844870.1. On May 9, 2018 this sequence version replaced NM_010134.3. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK157766.1, L12705.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849376, SAMN00849379 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGAGGAGA AGGATTCCAA GCCCAGCGAG ACGGCGGCGG AGGCGCAGAG ACAGCCGGAA 
CCCAGCTCCG GCGGCGGCTC TGGCGGCGGC AGCAGCCCGA GCGACTCGGA CACCGGCCGC 
CGGCGGGCTC TGATGCTGCC CGAGGTCCTA CAGGCGCCAG GCAACCACCA GCATCCACAT 
CGCATCACCA ACTTCTTCAT CGATAACATC CTGCGGCCTG AGTTTGGCCG CCGAAAGGAC 
GCGGGGACTT GCTGTGCGGG CGCGGGCGGA GCCAGGGGAG GCGAAGGCGG CGCTGGCACT 
ACCGAAGGAG GCGGCGGCGG CGCAGGCGGA GCCGAGCAGC TACTGGGCGC CAGGGAGTCC 
CGACCGAACC CAGCGTGCGC ACCCAGCGCG GGAGGAACGC TCTCCGCCGC CGCTGGCGAC 
CCTGCGGTCG ACGGAGAAGG AGGTTCCAAG ACGCTATCAC TTCACGGTGG TGCCAAAAAA 
CCCGGCGATC CTGGGGGTTC CTTGGATGGA GTGCTCAAAG CCCGGGGCTT GGGCGGCGGT 
GACCTGTCGG TGAGCTCCGA CTCGGACAGC TCTCAAGCCA GCGCCACTCT GGGCGCGCAG 
CCCATGCTCT GGCCCGCTTG GGTCTACTGC ACGCGCTATT CTGACCGGCC TTCTTCAGGT 
CCCAGGTCCC GAAAACCAAA GAAGAAGAAC CCTAACAAAG AGGACAAGCG GCCTCGCACA 
GCCTTCACTG CTGAGCAGCT CCAGAGGCTC AAGGCTGAGT TTCAGACCAA CAGGTACCTG 
ACAGAGCAGC GGCGCCAGAG TCTGGCACAG GAGCTCAGCC TGAACGAGTC TCAGATCAAG 
ATTTGGTTCC AGAACAAGCG GGCCAAAATC AAGAAAGCCA CGGGCAACAA GAACACTTTG 
GCGGTGCACC TCATGGCACA GGGCCTGTAC AACCATTCCA CCACGGCCAA GGAGGGCAAG 
TCGGACAGCG AGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_034264.1
CDS	832..1806
Translation	MEEKDSKPSETAAEAQRQPEPSSGGGSGGGSSPSDSDTGRRRALMLPEVLQAPGNHQHPHRITNFFIDNILRPEFGRRKDAGTCCAGAGGARGGEGGAGTTEGGGGGAGGAEQLLGARESRPNPACAPSAGGTLSAAAGDPAVDGEGGSKTLSLHGGAKKPGDPGGSLDGVLKARGLGGGDLSVSSDSDSSQASATLGAQPMLWPAWVYCTRYSDRPSSGPRSRKPKKKNPNKEDKRPRTAFTAEQLQRLKAEFQTNRYLTEQRRQSLAQELSLNESQIKIWFQNKRAKIKKATGNKNTLAVHLMAQGLYNHSTTAKEGKSDSE

Target ORF information:

RefSeq Version	NM_010134.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus engrailed 2 (En2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_010134.4

ORF Insert Sequence:

ATGGAGGAGA AGGATTCCAA GCCCAGCGAG ACGGCGGCGG AGGCGCAGAG ACAGCCGGAA 
CCCAGCTCCG GCGGCGGCTC TGGCGGCGGC AGCAGCCCGA GCGACTCGGA CACCGGCCGC 
CGGCGGGCTC TGATGCTGCC CGAGGTCCTA CAGGCGCCAG GCAACCACCA GCATCCACAT 
CGCATCACCA ACTTCTTCAT CGATAACATC CTGCGGCCTG AGTTTGGCCG CCGAAAGGAC 
GCGGGGACTT GCTGTGCGGG CGCGGGCGGA GCCAGGGGAG GCGAAGGCGG CGCTGGCACT 
ACCGAAGGAG GCGGCGGCGG CGCAGGCGGA GCCGAGCAGC TACTGGGCGC CAGGGAGTCC 
CGACCGAACC CAGCGTGCGC ACCCAGCGCG GGAGGAACGC TCTCCGCCGC CGCTGGCGAC 
CCTGCGGTCG ACGGAGAAGG AGGTTCCAAG ACGCTATCAC TTCACGGTGG TGCCAAAAAA 
CCCGGCGATC CTGGGGGTTC CTTGGATGGA GTGCTCAAAG CCCGGGGCTT GGGCGGCGGT 
GACCTGTCGG TGAGCTCCGA CTCGGACAGC TCTCAAGCCA GCGCCACTCT GGGCGCGCAG 
CCCATGCTCT GGCCCGCTTG GGTCTACTGC ACGCGCTATT CTGACCGGCC TTCTTCAGGT 
CCCAGGTCCC GAAAACCAAA GAAGAAGAAC CCTAACAAAG AGGACAAGCG GCCTCGCACA 
GCCTTCACTG CTGAGCAGCT CCAGAGGCTC AAGGCTGAGT TTCAGACCAA CAGGTACCTG 
ACAGAGCAGC GGCGCCAGAG TCTGGCACAG GAGCTCAGCC TGAACGAGTC TCAGATCAAG 
ATTTGGTTCC AGAACAAGCG GGCCAAAATC AAGAAAGCCA CGGGCAACAA GAACACTTTG 
GCGGTGCACC TCATGGCACA GGGCCTGTAC AACCATTCCA CCACGGCCAA GGAGGGCAAG 
TCGGACAGCG AGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Higher-Order Kidney Organogenesis from Pluripotent Stem Cells.
Cell stem cell21(6)730-746.e6(2017 Dec)
Taguchi A,Nishinakamura R

Increased signaling by the autism-related Engrailed-2 protein enhances dendritic branching and spine density, alters synaptic structural matching, and exaggerates protein synthesis.
PloS one12(8)e0181350(2017)
Soltani A,Lebrun S,Carpentier G,Zunino G,Chantepie S,Ma?za A,Bozzi Y,Desnos C,Darchen F,Stettler O

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En2 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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