Egf gene cDNA ORF clone, Mus musculus(house mouse)

mRNA	Protein	Name
NM_001329594.1	NP_001316523.1	pro-epidermal growth factor isoform 3
XM_011240010.2	XP_011238312.1	pro-epidermal growth factor isoform X1
XM_011240010.3	XP_011238312.1	pro-epidermal growth factor isoform X1
NM_010113.4	NP_034243.2	pro-epidermal growth factor isoform 1 preproprotein
XM_011240011.2	XP_011238313.1	pro-epidermal growth factor isoform X2
XM_011240011.3	XP_011238313.1	pro-epidermal growth factor isoform X2
NM_001310737.1	NP_001297666.1	pro-epidermal growth factor isoform 2
XM_006500990.3	XP_006501053.1	pro-epidermal growth factor isoform X3
XM_006500990.4	XP_006501053.1	pro-epidermal growth factor isoform X3

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu67603	NM_001310737.1 Latest version!	Mus musculus epidermal growth factor (Egf), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
OMu83574	NM_001329594.1 Latest version!	Mus musculus epidermal growth factor (Egf), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OMu31618	XM_006500990.4 Latest version!	Mus musculus epidermal growth factor (Egf), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OMu67604	XM_011240010.3 Latest version!	Mus musculus epidermal growth factor (Egf), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OMu67605	XM_011240011.3 Latest version!	Mus musculus epidermal growth factor (Egf), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OMu22638	NM_010113.4 Latest version!	Mus musculus epidermal growth factor (Egf), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
View Old Accession Versions >>
OMu67604	XM_011240010.2	Mus musculus epidermal growth factor (Egf), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$643.30 $919.00
OMu67605	XM_011240011.2	Mus musculus epidermal growth factor (Egf), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$643.30 $919.00
OMu31618	XM_006500990.3	Mus musculus epidermal growth factor (Egf), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$643.30 $919.00
Hide Old Accession Versions >>

Egf ( NM_001310737.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001297666.1 Mus musculus epidermal growth factor (Egf), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OMu67603
Clone ID Related Accession (Same CDS sequence)	NM_001310737.1
Accession Version	NM_001310737.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2151bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-11
Organism	Mus musculus(house mouse)
Product	pro-epidermal growth factor isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK157778.1, AK136439.1, AC098732.3 and BP761200.1. On Jul 18, 2015 this sequence version replaced XM_006500991.1. Transcript Variant: This variant (2) differs in the 5' UTR, lacks a portion of the 5' coding region, and initiates translation at a downstream start site compared to variant 1. The encoded isoform (2) has a shorter N-terminus than isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK136439.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCATTTTG ATGGAACAGA CTACAAAGTT CTGCTCAGCC GGCAGATGGG AATGGTTTTT 
GCCTTGGATT ATGACCCTGT GGAAAGCAAG ATATATTTTG CACAGACAGC CCTGAAGTGG 
ATAGAGAGGG CTAATATGGA TGGGTCCCAG CGAGAAAGAC TGATCACAGA AGGAGTAGAT 
ACGCTTGAAG GTCTTGCCCT GGACTGGATT GGCCGGAGAA TCTACTGGAC AGACAGTGGG 
AAGTCTGTTG TTGGAGGGAG CGATCTGAGC GGGAAGCATC ATCGAATAAT CATCCAGGAG 
AGAATCTCGA GGCCGCGAGG AATAGCTGTG CATCCAAGGG CCAGGAGACT GTTCTGGACG 
GACGTAGGGA TGTCTCCACG GATTGAAAGC GCTTCCCTTC AAGGTTCCGA CCGGGTGCTG 
ATAGCCAGCT CCAATCTACT GGAACCCAGT GGAATCACGA TTGACTACTT AACAGACACT 
TTGTACTGGT GTGACACCAA GAGGTCTGTG ATTGAAATGG CCAATCTGGA TGGCTCCAAA 
CGCCGAAGAC TTATCCAGAA CGACGTAGGT CACCCCTTCT CTCTAGCCGT GTTTGAGGAT 
CACCTGTGGG TCTCGGATTG GGCTATCCCA TCGGTAATAA GGGTGAACAA GAGGACTGGC 
CAAAACAGGG TACGTCTTCA AGGCAGCATG CTGAAGCCCT CGTCACTGGT TGTGGTCCAT 
CCATTGGCAA AACCAGGTGC AGATCCCTGC TTATACAGGA ATGGAGGCTG TGAACACATC 
TGCCAAGAGA GCCTGGGCAC AGCTCGGTGT TTGTGTCGTG AAGGTTTTGT GAAGGCCTGG 
GATGGGAAAA TGTGTCTCCC TCAGGATTAT CCAATCCTGT CAGGTGAAAA TGCTGATCTT 
AGTAAAGAGG TGACATCACT GAGCAACTCC ACTCAGGCTG AAGTACCAGA CGATGATGGG 
ACAGAATCTT CCACACTAGT GGCTGAAATC ATGGTGTCAG GCATGAACTA TGAAGATGAC 
TGTGGTCCCG GGGGGTGTGG AAGCCATGCT CGATGCGTTT CAGACGGAGA GACTGCTGAG 
TGTCAGTGTC TGAAAGGGTT TGCCAGGGAT GGAAACCTGT GTTCTGATAT AGATGAGTGT 
GTGCTGGCTA GATCGGACTG CCCCAGCACC TCGTCCAGGT GCATCAACAC TGAAGGTGGC 
TACGTCTGCA GATGCTCAGA AGGCTACGAA GGAGACGGGA TCTCCTGTTT CGATATTGAC 
GAGTGCCAGC GGGGGGCGCA CAACTGCGGT GAGAATGCCG CCTGCACCAA CACGGAGGGA 
GGCTACAACT GCACCTGCGC AGGCCGCCCA TCCTCGCCCG GACTGAGTTG CCCTGACTCT 
ACCGCACCCT CTCTCCTTGG GGAAGATGGC CACCATTTGG ACCGAAATAG TTATCCAGGA 
TGCCCATCCT CATATGATGG ATACTGCCTC AATGGTGGCG TGTGCATGCA TATTGAATCA 
CTGGACAGCT ACACATGCAA CTGTGTTATT GGCTATTCTG GGGATCGATG CCAGACTCGA 
GACCTACGAT GGTGGGAACT GCGTCATGCT GGCTACGGGC AGAAGCATGA CATCATGGTG 
GTGGCTGTCT GCATGGTGGC ACTGGTCCTG CTGCTCGTCT TGGGGATGTG GGGGACTTAC 
TACTACAGGA CTCGGAAGCA GCTATCAAAC CCCCCAAAGA ACCCTTGTGA TGAGCCAAGC 
GGAAGTGTGA GCAGCAGCGG ACCCAACAGC AGCAGCGGGG CAGCTGTGGC TTCTTGTCCC 
CAACCTTGGT TTGTGGTCCT AGAGAAACAC CAAGACCCCA AGAATGGGAG TCTGCCTGCG 
GATGGTACGA ATGGTGCAGT AGTAGATGCT GGCCTGTCTC CCTCCCTGCA GCTCGGGTCA 
GTGCATCTGA CTTCATGGAG ACAGAAGCCC CACATAGATG GAATGGGCAC AGGGCAAAGC 
TGCTGGATTC CACCATCAAG TGACAGAGGA CCCCAGGAAA TAGAGGGAAA CTCCCACCTA 
CCCTCCTACA GACCTGTGGG GCCGGAGAAG CTGCATTCTC TCCAGTCAGC TAATGGATCG 
TGTCACGAAA GGGCTCCAGA CCTGCCACGG CAGACAGAGC CAGTTCAGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001297666.1
CDS	453..2603
Translation	MHFDGTDYKVLLSRQMGMVFALDYDPVESKIYFAQTALKWIERANMDGSQRERLITEGVDTLEGLALDWIGRRIYWTDSGKSVVGGSDLSGKHHRIIIQERISRPRGIAVHPRARRLFWTDVGMSPRIESASLQGSDRVLIASSNLLEPSGITIDYLTDTLYWCDTKRSVIEMANLDGSKRRRLIQNDVGHPFSLAVFEDHLWVSDWAIPSVIRVNKRTGQNRVRLQGSMLKPSSLVVVHPLAKPGADPCLYRNGGCEHICQESLGTARCLCREGFVKAWDGKMCLPQDYPILSGENADLSKEVTSLSNSTQAEVPDDDGTESSTLVAEIMVSGMNYEDDCGPGGCGSHARCVSDGETAECQCLKGFARDGNLCSDIDECVLARSDCPSTSSRCINTEGGYVCRCSEGYEGDGISCFDIDECQRGAHNCGENAACTNTEGGYNCTCAGRPSSPGLSCPDSTAPSLLGEDGHHLDRNSYPGCPSSYDGYCLNGGVCMHIESLDSYTCNCVIGYSGDRCQTRDLRWWELRHAGYGQKHDIMVVAVCMVALVLLLVLGMWGTYYYRTRKQLSNPPKNPCDEPSGSVSSSGPNSSSGAAVASCPQPWFVVLEKHQDPKNGSLPADGTNGAVVDAGLSPSLQLGSVHLTSWRQKPHIDGMGTGQSCWIPPSSDRGPQEIEGNSHLPSYRPVGPEKLHSLQSANGSCHERAPDLPRQTEPVQ

Target ORF information:

RefSeq Version	NM_001310737.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus epidermal growth factor (Egf), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001310737.1

ORF Insert Sequence:

ATGCATTTTG ATGGAACAGA CTACAAAGTT CTGCTCAGCC GGCAGATGGG AATGGTTTTT 
GCCTTGGATT ATGACCCTGT GGAAAGCAAG ATATATTTTG CACAGACAGC CCTGAAGTGG 
ATAGAGAGGG CTAATATGGA TGGGTCCCAG CGAGAAAGAC TGATCACAGA AGGAGTAGAT 
ACGCTTGAAG GTCTTGCCCT GGACTGGATT GGCCGGAGAA TCTACTGGAC AGACAGTGGG 
AAGTCTGTTG TTGGAGGGAG CGATCTGAGC GGGAAGCATC ATCGAATAAT CATCCAGGAG 
AGAATCTCGA GGCCGCGAGG AATAGCTGTG CATCCAAGGG CCAGGAGACT GTTCTGGACG 
GACGTAGGGA TGTCTCCACG GATTGAAAGC GCTTCCCTTC AAGGTTCCGA CCGGGTGCTG 
ATAGCCAGCT CCAATCTACT GGAACCCAGT GGAATCACGA TTGACTACTT AACAGACACT 
TTGTACTGGT GTGACACCAA GAGGTCTGTG ATTGAAATGG CCAATCTGGA TGGCTCCAAA 
CGCCGAAGAC TTATCCAGAA CGACGTAGGT CACCCCTTCT CTCTAGCCGT GTTTGAGGAT 
CACCTGTGGG TCTCGGATTG GGCTATCCCA TCGGTAATAA GGGTGAACAA GAGGACTGGC 
CAAAACAGGG TACGTCTTCA AGGCAGCATG CTGAAGCCCT CGTCACTGGT TGTGGTCCAT 
CCATTGGCAA AACCAGGTGC AGATCCCTGC TTATACAGGA ATGGAGGCTG TGAACACATC 
TGCCAAGAGA GCCTGGGCAC AGCTCGGTGT TTGTGTCGTG AAGGTTTTGT GAAGGCCTGG 
GATGGGAAAA TGTGTCTCCC TCAGGATTAT CCAATCCTGT CAGGTGAAAA TGCTGATCTT 
AGTAAAGAGG TGACATCACT GAGCAACTCC ACTCAGGCTG AAGTACCAGA CGATGATGGG 
ACAGAATCTT CCACACTAGT GGCTGAAATC ATGGTGTCAG GCATGAACTA TGAAGATGAC 
TGTGGTCCCG GGGGGTGTGG AAGCCATGCT CGATGCGTTT CAGACGGAGA GACTGCTGAG 
TGTCAGTGTC TGAAAGGGTT TGCCAGGGAT GGAAACCTGT GTTCTGATAT AGATGAGTGT 
GTGCTGGCTA GATCGGACTG CCCCAGCACC TCGTCCAGGT GCATCAACAC TGAAGGTGGC 
TACGTCTGCA GATGCTCAGA AGGCTACGAA GGAGACGGGA TCTCCTGTTT CGATATTGAC 
GAGTGCCAGC GGGGGGCGCA CAACTGCGGT GAGAATGCCG CCTGCACCAA CACGGAGGGA 
GGCTACAACT GCACCTGCGC AGGCCGCCCA TCCTCGCCCG GACTGAGTTG CCCTGACTCT 
ACCGCACCCT CTCTCCTTGG GGAAGATGGC CACCATTTGG ACCGAAATAG TTATCCAGGA 
TGCCCATCCT CATATGATGG ATACTGCCTC AATGGTGGCG TGTGCATGCA TATTGAATCA 
CTGGACAGCT ACACATGCAA CTGTGTTATT GGCTATTCTG GGGATCGATG CCAGACTCGA 
GACCTACGAT GGTGGGAACT GCGTCATGCT GGCTACGGGC AGAAGCATGA CATCATGGTG 
GTGGCTGTCT GCATGGTGGC ACTGGTCCTG CTGCTCGTCT TGGGGATGTG GGGGACTTAC 
TACTACAGGA CTCGGAAGCA GCTATCAAAC CCCCCAAAGA ACCCTTGTGA TGAGCCAAGC 
GGAAGTGTGA GCAGCAGCGG ACCCAACAGC AGCAGCGGGG CAGCTGTGGC TTCTTGTCCC 
CAACCTTGGT TTGTGGTCCT AGAGAAACAC CAAGACCCCA AGAATGGGAG TCTGCCTGCG 
GATGGTACGA ATGGTGCAGT AGTAGATGCT GGCCTGTCTC CCTCCCTGCA GCTCGGGTCA 
GTGCATCTGA CTTCATGGAG ACAGAAGCCC CACATAGATG GAATGGGCAC AGGGCAAAGC 
TGCTGGATTC CACCATCAAG TGACAGAGGA CCCCAGGAAA TAGAGGGAAA CTCCCACCTA 
CCCTCCTACA GACCTGTGGG GCCGGAGAAG CTGCATTCTC TCCAGTCAGC TAATGGATCG 
TGTCACGAAA GGGCTCCAGA CCTGCCACGG CAGACAGAGC CAGTTCAGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Egf ( NM_001329594.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001316523.1 Mus musculus epidermal growth factor (Egf), transcript variant 3, mRNA.

Price & Availability↑

CloneID	OMu83574
Clone ID Related Accession (Same CDS sequence)	NM_001329594.1
Accession Version	NM_001329594.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2028bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-11
Organism	Mus musculus(house mouse)
Product	pro-epidermal growth factor isoform 3
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC098732.3, BB635280.1, AK085707.1 and BP761200.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK088408.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCATTTTG ATGGAACAGA CTACAAAGTT CTGCTCAGCC GGCAGATGGG AATGGTTTTT 
GCCTTGGATT ATGACCCTGT GGAAAGCAAG ATATATTTTG CACAGACAGC CCTGAAGTGG 
ATAGAGAGGG CTAATATGGA TGGGTCCCAG CGAGAAAGAC TGATCACAGA AGGAGTAGAT 
ACGCTTGAAG GTCTTGCCCT GGACTGGATT GGCCGGAGAA TCTACTGGAC AGACAGTGGG 
AAGTCTGTTG TTGGAGGGAG CGATCTGAGC GGGAAGCATC ATCGAATAAT CATCCAGGAG 
AGAATCTCGA GGCCGCGAGG AATAGCTGTG CATCCAAGGG CCAGGAGACT GTTCTGGACG 
GACGTAGGGA TGTCTCCACG GATTGAAAGC GCTTCCCTTC AAGGTTCCGA CCGGGTGCTG 
ATAGCCAGCT CCAATCTACT GGAACCCAGT GGAATCACGA TTGACTACTT AACAGACACT 
TTGTACTGGT GTGACACCAA GAGGTCTGTG ATTGAAATGG CCAATCTGGA TGGCTCCAAA 
CGCCGAAGAC TTATCCAGAA CGACGTAGGT CACCCCTTCT CTCTAGCCGT GTTTGAGGAT 
CACCTGTGGG TCTCGGATTG GGCTATCCCA TCGGTAATAA GGGTGAACAA GAGGACTGGC 
CAAAACAGGG TACGTCTTCA AGGCAGCATG CTGAAGCCCT CGTCACTGGT TGTGGTCCAT 
CCATTGGCAA AACCAGGTGC AGATCCCTGC TTATACAGGA ATGGAGGCTG TGAACACATC 
TGCCAAGAGA GCCTGGGCAC AGCTCGGTGT TTGTGTCGTG AAGGTTTTGT GAAGGCCTGG 
GATGGGAAAA TGTGTCTCCC TCAGGATTAT CCAATCCTGT CAGGTGAAAA TGCTGATCTT 
AGTAAAGAGG TGACATCACT GAGCAACTCC ACTCAGGCTG AAGTACCAGA CGATGATGGG 
ACAGAATCTT CCACACTAGT GGCTGAAATC ATGGTGTCAG GCATGAACTA TGAAGATGAC 
TGTGGTCCCG GGGGGTGTGG AAGCCATGCT CGATGCGTTT CAGACGGAGA GACTGCTGAG 
TGTCAGTGTC TGAAAGGGTT TGCCAGGGAT GGAAACCTGT GTTCTGATAT AGATGAGTGT 
GTGCTGGCTA GATCGGACTG CCCCAGCACC TCGTCCAGGT GCATCAACAC TGAAGGTGGC 
TACGTCTGCA GATGCTCAGA AGGCTACGAA GGAGACGGGA TCTCCTGTTT CGACTCTACC 
GCACCCTCTC TCCTTGGGGA AGATGGCCAC CATTTGGACC GAAATAGTTA TCCAGGATGC 
CCATCCTCAT ATGATGGATA CTGCCTCAAT GGTGGCGTGT GCATGCATAT TGAATCACTG 
GACAGCTACA CATGCAACTG TGTTATTGGC TATTCTGGGG ATCGATGCCA GACTCGAGAC 
CTACGATGGT GGGAACTGCG TCATGCTGGC TACGGGCAGA AGCATGACAT CATGGTGGTG 
GCTGTCTGCA TGGTGGCACT GGTCCTGCTG CTCGTCTTGG GGATGTGGGG GACTTACTAC 
TACAGGACTC GGAAGCAGCT ATCAAACCCC CCAAAGAACC CTTGTGATGA GCCAAGCGGA 
AGTGTGAGCA GCAGCGGACC CAACAGCAGC AGCGGGGCAG CTGTGGCTTC TTGTCCCCAA 
CCTTGGTTTG TGGTCCTAGA GAAACACCAA GACCCCAAGA ATGGGAGTCT GCCTGCGGAT 
GGTACGAATG GTGCAGTAGT AGATGCTGGC CTGTCTCCCT CCCTGCAGCT CGGGTCAGTG 
CATCTGACTT CATGGAGACA GAAGCCCCAC ATAGATGGAA TGGGCACAGG GCAAAGCTGC 
TGGATTCCAC CATCAAGTGA CAGAGGACCC CAGGAAATAG AGGGAAACTC CCACCTACCC 
TCCTACAGAC CTGTGGGGCC GGAGAAGCTG CATTCTCTCC AGTCAGCTAA TGGATCGTGT 
CACGAAAGGG CTCCAGACCT GCCACGGCAG ACAGAGCCAG TTCAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001316523.1
CDS	453..2480
Translation	MHFDGTDYKVLLSRQMGMVFALDYDPVESKIYFAQTALKWIERANMDGSQRERLITEGVDTLEGLALDWIGRRIYWTDSGKSVVGGSDLSGKHHRIIIQERISRPRGIAVHPRARRLFWTDVGMSPRIESASLQGSDRVLIASSNLLEPSGITIDYLTDTLYWCDTKRSVIEMANLDGSKRRRLIQNDVGHPFSLAVFEDHLWVSDWAIPSVIRVNKRTGQNRVRLQGSMLKPSSLVVVHPLAKPGADPCLYRNGGCEHICQESLGTARCLCREGFVKAWDGKMCLPQDYPILSGENADLSKEVTSLSNSTQAEVPDDDGTESSTLVAEIMVSGMNYEDDCGPGGCGSHARCVSDGETAECQCLKGFARDGNLCSDIDECVLARSDCPSTSSRCINTEGGYVCRCSEGYEGDGISCFDSTAPSLLGEDGHHLDRNSYPGCPSSYDGYCLNGGVCMHIESLDSYTCNCVIGYSGDRCQTRDLRWWELRHAGYGQKHDIMVVAVCMVALVLLLVLGMWGTYYYRTRKQLSNPPKNPCDEPSGSVSSSGPNSSSGAAVASCPQPWFVVLEKHQDPKNGSLPADGTNGAVVDAGLSPSLQLGSVHLTSWRQKPHIDGMGTGQSCWIPPSSDRGPQEIEGNSHLPSYRPVGPEKLHSLQSANGSCHERAPDLPRQTEPVQ

Target ORF information:

RefSeq Version	NM_001329594.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus epidermal growth factor (Egf), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001329594.1

ORF Insert Sequence:

ATGCATTTTG ATGGAACAGA CTACAAAGTT CTGCTCAGCC GGCAGATGGG AATGGTTTTT 
GCCTTGGATT ATGACCCTGT GGAAAGCAAG ATATATTTTG CACAGACAGC CCTGAAGTGG 
ATAGAGAGGG CTAATATGGA TGGGTCCCAG CGAGAAAGAC TGATCACAGA AGGAGTAGAT 
ACGCTTGAAG GTCTTGCCCT GGACTGGATT GGCCGGAGAA TCTACTGGAC AGACAGTGGG 
AAGTCTGTTG TTGGAGGGAG CGATCTGAGC GGGAAGCATC ATCGAATAAT CATCCAGGAG 
AGAATCTCGA GGCCGCGAGG AATAGCTGTG CATCCAAGGG CCAGGAGACT GTTCTGGACG 
GACGTAGGGA TGTCTCCACG GATTGAAAGC GCTTCCCTTC AAGGTTCCGA CCGGGTGCTG 
ATAGCCAGCT CCAATCTACT GGAACCCAGT GGAATCACGA TTGACTACTT AACAGACACT 
TTGTACTGGT GTGACACCAA GAGGTCTGTG ATTGAAATGG CCAATCTGGA TGGCTCCAAA 
CGCCGAAGAC TTATCCAGAA CGACGTAGGT CACCCCTTCT CTCTAGCCGT GTTTGAGGAT 
CACCTGTGGG TCTCGGATTG GGCTATCCCA TCGGTAATAA GGGTGAACAA GAGGACTGGC 
CAAAACAGGG TACGTCTTCA AGGCAGCATG CTGAAGCCCT CGTCACTGGT TGTGGTCCAT 
CCATTGGCAA AACCAGGTGC AGATCCCTGC TTATACAGGA ATGGAGGCTG TGAACACATC 
TGCCAAGAGA GCCTGGGCAC AGCTCGGTGT TTGTGTCGTG AAGGTTTTGT GAAGGCCTGG 
GATGGGAAAA TGTGTCTCCC TCAGGATTAT CCAATCCTGT CAGGTGAAAA TGCTGATCTT 
AGTAAAGAGG TGACATCACT GAGCAACTCC ACTCAGGCTG AAGTACCAGA CGATGATGGG 
ACAGAATCTT CCACACTAGT GGCTGAAATC ATGGTGTCAG GCATGAACTA TGAAGATGAC 
TGTGGTCCCG GGGGGTGTGG AAGCCATGCT CGATGCGTTT CAGACGGAGA GACTGCTGAG 
TGTCAGTGTC TGAAAGGGTT TGCCAGGGAT GGAAACCTGT GTTCTGATAT AGATGAGTGT 
GTGCTGGCTA GATCGGACTG CCCCAGCACC TCGTCCAGGT GCATCAACAC TGAAGGTGGC 
TACGTCTGCA GATGCTCAGA AGGCTACGAA GGAGACGGGA TCTCCTGTTT CGACTCTACC 
GCACCCTCTC TCCTTGGGGA AGATGGCCAC CATTTGGACC GAAATAGTTA TCCAGGATGC 
CCATCCTCAT ATGATGGATA CTGCCTCAAT GGTGGCGTGT GCATGCATAT TGAATCACTG 
GACAGCTACA CATGCAACTG TGTTATTGGC TATTCTGGGG ATCGATGCCA GACTCGAGAC 
CTACGATGGT GGGAACTGCG TCATGCTGGC TACGGGCAGA AGCATGACAT CATGGTGGTG 
GCTGTCTGCA TGGTGGCACT GGTCCTGCTG CTCGTCTTGG GGATGTGGGG GACTTACTAC 
TACAGGACTC GGAAGCAGCT ATCAAACCCC CCAAAGAACC CTTGTGATGA GCCAAGCGGA 
AGTGTGAGCA GCAGCGGACC CAACAGCAGC AGCGGGGCAG CTGTGGCTTC TTGTCCCCAA 
CCTTGGTTTG TGGTCCTAGA GAAACACCAA GACCCCAAGA ATGGGAGTCT GCCTGCGGAT 
GGTACGAATG GTGCAGTAGT AGATGCTGGC CTGTCTCCCT CCCTGCAGCT CGGGTCAGTG 
CATCTGACTT CATGGAGACA GAAGCCCCAC ATAGATGGAA TGGGCACAGG GCAAAGCTGC 
TGGATTCCAC CATCAAGTGA CAGAGGACCC CAGGAAATAG AGGGAAACTC CCACCTACCC 
TCCTACAGAC CTGTGGGGCC GGAGAAGCTG CATTCTCTCC AGTCAGCTAA TGGATCGTGT 
CACGAAAGGG CTCCAGACCT GCCACGGCAG ACAGAGCCAG TTCAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Egf ( XM_006500990.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006501053.1 Mus musculus epidermal growth factor (Egf), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OMu31618
Clone ID Related Accession (Same CDS sequence)	XM_006500990.3 , XM_006500990.4
Accession Version	XM_006500990.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3531bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	pro-epidermal growth factor isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000069.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006500990.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCCTGGG GCCGAAGGCC AACCTGGCTG TTGCTCGCCT TCCTGCTGGT GTTTTTAAAG 
ATTAGCATAC TCAGCGTCAC AGCATGGCAG ACCGGGAACT GTCAGCCAGG TCCTCTCGAG 
AGAAGCGAGA GAAGCGGGAC TTGTGCCGGT CCTGCCCCCT TCCTAGTTTT CTCACAAGGA 
AAGAGCATCT CTCGGATTGA CCCAGATGGA ACAAATCACC AGCAATTGGT GGTGGATGCT 
GGCATCTCAG CAGACATGGA TATTCATTAT AAAAAAGAGA GACTCTATTG GGTGGATGTA 
GAAAGACAAG TTTTGCTAAG AGTTTTCCTT AACGGGACAG GACTAGAGAA AGTGTGCAAT 
GTAGAGAGGA AGGTGTCTGG GCTGGCCATA GACTGGATAG ATGATGAAGT TCTCTGGGTA 
GACCAACAGA ACGGAGTCAT CACCGTAACA GATATGACAG GGAAAAATTC CCGAGTTCTT 
CTAAGTTCCT TAAAACATCC GTCAAATATA GCAGTGGATC CAATAGAGAG GTTGATGTTT 
TGGTCTTCAG AGGTGACCGG CAGCCTTCAC AGAGCACACC TCAAAGGTGT TGATGTAAAA 
ACACTGCTGG AGACAGGGGG AATATCGGTG CTGACTCTGG ATGTCCTGGA CAAACGGCTC 
TTCTGGGTTC AGGACAGTGG CGAAGGAAGC CACGCTTACA TTCATTCCTG TGATTATGAG 
GGTGGCTCCG TCCGTCTTAT CAGGCATCAA GCACGGCACA GTTTGTCTTC AATGGCCTTT 
TTTGGTGATC GGATCTTCTA CTCAGTGTTG AAAAGCAAGG CGATTTGGAT AGCCAACAAA 
CACACGGGGA AGGACACGGT CAGGATTAAC CTCCATCCAT CCTTTGTGAC ACCTGGAAAA 
CTGATGGTAG TACACCCTCG TGCACAGCCC AGGACAGAGG ACGCTGCTAA GGATCCTGAC 
CCCGAACTTC TCAAACAGAG GGGAAGACCA TGCCGCTTCG GTCTCTGTGA GCGAGACCCC 
AAGTCCCACT CGAGCGCATG CGCTGAGGGC TACACGTTAA GCCGAGACCG GAAGTACTGC 
GAAGATGTCA ATGAATGTGC CACTCAGAAT CACGGCTGTA CTCTTGGGTG TGAAAACACC 
CCTGGATCCT ATCACTGCAC ATGCCCCACA GGATTTGTTC TGCTTCCTGA TGGGAAACAA 
TGTCACGAAC TTGTTTCCTG CCCAGGCAAC GTATCAAAGT GCAGTCATGG CTGTGTCCTG 
ACATCAGATG GTCCCCGGTG CATCTGTCCT GCAGGTTCAG TGCTTGGGAG AGATGGGAAG 
ACTTGCACTG GTTGTTCATC GCCTGACAAT GGTGGATGCA GCCAGATCTG TCTTCCTCTC 
AGGCCAGGAT CCTGGGAATG TGATTGCTTT CCTGGGTATG ACCTACAGTC AGACCGAAAG 
AGCTGTGCAG CTTCAGGACC ACAGCCACTT TTACTGTTTG CAAATTCCCA GGACATCCGA 
CACATGCATT TTGATGGAAC AGACTACAAA GTTCTGCTCA GCCGGCAGAT GGGAATGGTT 
TTTGCCTTGG ATTATGACCC TGTGGAAAGC AAGATATATT TTGCACAGAC AGCCCTGAAG 
TGGATAGAGA GGGCTAATAT GGATGGGTCC CAGCGAGAAA GACTGATCAC AGAAGGAGTA 
GATACGCTTG AAGGTCTTGC CCTGGACTGG ATTGGCCGGA GAATCTACTG GACAGACAGT 
GGGAAGTCTG TTGTTGGAGG GAGCGATCTG AGCGGGAAGC ATCATCGAAT AATCATCCAG 
GAGAGAATCT CGAGGCCGCG AGGAATAGCT GTGCATCCAA GGGCCAGGAG ACTGTTCTGG 
ACGGACGTAG GGATGTCTCC ACGGATTGAA AGCGCTTCCC TTCAAGGTTC CGACCGGGTG 
CTGATAGCCA GCTCCAATCT ACTGGAACCC AGTGGAATCA CGATTGACTA CTTAACAGAC 
ACTTTGTACT GGTGTGACAC CAAGAGGTCT GTGATTGAAA TGGCCAATCT GGATGGCTCC 
AAACGCCGAA GACTTATCCA GAACGACGTA GGTCACCCCT TCTCTCTAGC CGTGTTTGAG 
GATCACCTGT GGGTCTCGGA TTGGGCTATC CCATCGGTAA TAAGGGTGAA CAAGAGGACT 
GGCCAAAACA GGGTACGTCT TCAAGGCAGC ATGCTGAAGC CCTCGTCACT GGTTGTGGTC 
CATCCATTGG CAAAACCAGG TGCAGATCCC TGCTTATACA GGAATGGAGG CTGTGAACAC 
ATCTGCCAAG AGAGCCTGGG CACAGCTCGG TGTTTGTGTC GTGAAGGTTT TGTGAAGGCC 
TGGGATGGGA AAATGTGTCT CCCTCAGGAT TATCCAATCC TGTCAGGTGA AAATGCTGAT 
CTTAGTAAAG AGGTGACATC ACTGAGCAAC TCCACTCAGG CTGAAGTACC AGACGATGAT 
GGGACAGAAT CTTCCACACT AGTGGCTGAA ATCATGGTGT CAGGCATGAA CTATGAAGAT 
GACTGTGGTC CCGGGGGGTG TGGAAGCCAT GCTCGATGCG TTTCAGACGG AGAGACTGCT 
GAGTGTCAGT GTCTGAAAGG GTTTGCCAGG GATGGAAACC TGTGTTCTGA TATAGATGAG 
TGTGTGCTGG CTAGATCGGA CTGCCCCAGC ACCTCGTCCA GGTGCATCAA CACTGAAGGT 
GGCTACGTCT GCAGATGCTC AGAAGGCTAC GAAGGAGACG GGATCTCCTG TTTCGACTCT 
ACCGCACCCT CTCTCCTTGG GGAAGATGGC CACCATTTGG ACCGAAATAG TTATCCAGGA 
TGCCCATCCT CATATGATGG ATACTGCCTC AATGGTGGCG TGTGCATGCA TATTGAATCA 
CTGGACAGCT ACACATGCAA CTGTGTTATT GGCTATTCTG GGGATCGATG CCAGACTCGA 
GACCTACGAT GGTGGGAACT GCGTCATGCT GGCTACGGGC AGAAGCATGA CATCATGGTG 
GTGGCTGTCT GCATGGTGGC ACTGGTCCTG CTGCTCGTCT TGGGGATGTG GGGGACTTAC 
TACTACAGGA CTCGGAAGCA GCTATCAAAC CCCCCAAAGA ACCCTTGTGA TGAGCCAAGC 
GGAAGTGTGA GCAGCAGCGG ACCCAACAGC AGCAGCGGGG CAGCTGTGGC TTCTTGTCCC 
CAACCTTGGT TTGTGGTCCT AGAGAAACAC CAAGACCCCA AGAATGGGAG TCTGCCTGCG 
GATGGTACGA ATGGTGCAGT AGTAGATGCT GGCCTGTCTC CCTCCCTGCA GCTCGGGTCA 
GTGCATCTGA CTTCATGGAG ACAGAAGCCC CACATAGATG GAATGGGCAC AGGGCAAAGC 
TGCTGGATTC CACCATCAAG TGACAGAGGA CCCCAGGAAA TAGAGGGAAA CTCCCACCTA 
CCCTCCTACA GACCTGTGGG GCCGGAGAAG CTGCATTCTC TCCAGTCAGC TAATGGATCG 
TGTCACGAAA GGGCTCCAGA CCTGCCACGG CAGACAGAGC CAGTTCAGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006501053.1
CDS	135..3665
Translation	MPWGRRPTWLLLAFLLVFLKISILSVTAWQTGNCQPGPLERSERSGTCAGPAPFLVFSQGKSISRIDPDGTNHQQLVVDAGISADMDIHYKKERLYWVDVERQVLLRVFLNGTGLEKVCNVERKVSGLAIDWIDDEVLWVDQQNGVITVTDMTGKNSRVLLSSLKHPSNIAVDPIERLMFWSSEVTGSLHRAHLKGVDVKTLLETGGISVLTLDVLDKRLFWVQDSGEGSHAYIHSCDYEGGSVRLIRHQARHSLSSMAFFGDRIFYSVLKSKAIWIANKHTGKDTVRINLHPSFVTPGKLMVVHPRAQPRTEDAAKDPDPELLKQRGRPCRFGLCERDPKSHSSACAEGYTLSRDRKYCEDVNECATQNHGCTLGCENTPGSYHCTCPTGFVLLPDGKQCHELVSCPGNVSKCSHGCVLTSDGPRCICPAGSVLGRDGKTCTGCSSPDNGGCSQICLPLRPGSWECDCFPGYDLQSDRKSCAASGPQPLLLFANSQDIRHMHFDGTDYKVLLSRQMGMVFALDYDPVESKIYFAQTALKWIERANMDGSQRERLITEGVDTLEGLALDWIGRRIYWTDSGKSVVGGSDLSGKHHRIIIQERISRPRGIAVHPRARRLFWTDVGMSPRIESASLQGSDRVLIASSNLLEPSGITIDYLTDTLYWCDTKRSVIEMANLDGSKRRRLIQNDVGHPFSLAVFEDHLWVSDWAIPSVIRVNKRTGQNRVRLQGSMLKPSSLVVVHPLAKPGADPCLYRNGGCEHICQESLGTARCLCREGFVKAWDGKMCLPQDYPILSGENADLSKEVTSLSNSTQAEVPDDDGTESSTLVAEIMVSGMNYEDDCGPGGCGSHARCVSDGETAECQCLKGFARDGNLCSDIDECVLARSDCPSTSSRCINTEGGYVCRCSEGYEGDGISCFDSTAPSLLGEDGHHLDRNSYPGCPSSYDGYCLNGGVCMHIESLDSYTCNCVIGYSGDRCQTRDLRWWELRHAGYGQKHDIMVVAVCMVALVLLLVLGMWGTYYYRTRKQLSNPPKNPCDEPSGSVSSSGPNSSSGAAVASCPQPWFVVLEKHQDPKNGSLPADGTNGAVVDAGLSPSLQLGSVHLTSWRQKPHIDGMGTGQSCWIPPSSDRGPQEIEGNSHLPSYRPVGPEKLHSLQSANGSCHERAPDLPRQTEPVQ

Target ORF information:

RefSeq Version	XM_006500990.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus epidermal growth factor (Egf), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006500990.4

ORF Insert Sequence:

ATGCCCTGGG GCCGAAGGCC AACCTGGCTG TTGCTCGCCT TCCTGCTGGT GTTTTTAAAG 
ATTAGCATAC TCAGCGTCAC AGCATGGCAG ACCGGGAACT GTCAGCCAGG TCCTCTCGAG 
AGAAGCGAGA GAAGCGGGAC TTGTGCCGGT CCTGCCCCCT TCCTAGTTTT CTCACAAGGA 
AAGAGCATCT CTCGGATTGA CCCAGATGGA ACAAATCACC AGCAATTGGT GGTGGATGCT 
GGCATCTCAG CAGACATGGA TATTCATTAT AAAAAAGAGA GACTCTATTG GGTGGATGTA 
GAAAGACAAG TTTTGCTAAG AGTTTTCCTT AACGGGACAG GACTAGAGAA AGTGTGCAAT 
GTAGAGAGGA AGGTGTCTGG GCTGGCCATA GACTGGATAG ATGATGAAGT TCTCTGGGTA 
GACCAACAGA ACGGAGTCAT CACCGTAACA GATATGACAG GGAAAAATTC CCGAGTTCTT 
CTAAGTTCCT TAAAACATCC GTCAAATATA GCAGTGGATC CAATAGAGAG GTTGATGTTT 
TGGTCTTCAG AGGTGACCGG CAGCCTTCAC AGAGCACACC TCAAAGGTGT TGATGTAAAA 
ACACTGCTGG AGACAGGGGG AATATCGGTG CTGACTCTGG ATGTCCTGGA CAAACGGCTC 
TTCTGGGTTC AGGACAGTGG CGAAGGAAGC CACGCTTACA TTCATTCCTG TGATTATGAG 
GGTGGCTCCG TCCGTCTTAT CAGGCATCAA GCACGGCACA GTTTGTCTTC AATGGCCTTT 
TTTGGTGATC GGATCTTCTA CTCAGTGTTG AAAAGCAAGG CGATTTGGAT AGCCAACAAA 
CACACGGGGA AGGACACGGT CAGGATTAAC CTCCATCCAT CCTTTGTGAC ACCTGGAAAA 
CTGATGGTAG TACACCCTCG TGCACAGCCC AGGACAGAGG ACGCTGCTAA GGATCCTGAC 
CCCGAACTTC TCAAACAGAG GGGAAGACCA TGCCGCTTCG GTCTCTGTGA GCGAGACCCC 
AAGTCCCACT CGAGCGCATG CGCTGAGGGC TACACGTTAA GCCGAGACCG GAAGTACTGC 
GAAGATGTCA ATGAATGTGC CACTCAGAAT CACGGCTGTA CTCTTGGGTG TGAAAACACC 
CCTGGATCCT ATCACTGCAC ATGCCCCACA GGATTTGTTC TGCTTCCTGA TGGGAAACAA 
TGTCACGAAC TTGTTTCCTG CCCAGGCAAC GTATCAAAGT GCAGTCATGG CTGTGTCCTG 
ACATCAGATG GTCCCCGGTG CATCTGTCCT GCAGGTTCAG TGCTTGGGAG AGATGGGAAG 
ACTTGCACTG GTTGTTCATC GCCTGACAAT GGTGGATGCA GCCAGATCTG TCTTCCTCTC 
AGGCCAGGAT CCTGGGAATG TGATTGCTTT CCTGGGTATG ACCTACAGTC AGACCGAAAG 
AGCTGTGCAG CTTCAGGACC ACAGCCACTT TTACTGTTTG CAAATTCCCA GGACATCCGA 
CACATGCATT TTGATGGAAC AGACTACAAA GTTCTGCTCA GCCGGCAGAT GGGAATGGTT 
TTTGCCTTGG ATTATGACCC TGTGGAAAGC AAGATATATT TTGCACAGAC AGCCCTGAAG 
TGGATAGAGA GGGCTAATAT GGATGGGTCC CAGCGAGAAA GACTGATCAC AGAAGGAGTA 
GATACGCTTG AAGGTCTTGC CCTGGACTGG ATTGGCCGGA GAATCTACTG GACAGACAGT 
GGGAAGTCTG TTGTTGGAGG GAGCGATCTG AGCGGGAAGC ATCATCGAAT AATCATCCAG 
GAGAGAATCT CGAGGCCGCG AGGAATAGCT GTGCATCCAA GGGCCAGGAG ACTGTTCTGG 
ACGGACGTAG GGATGTCTCC ACGGATTGAA AGCGCTTCCC TTCAAGGTTC CGACCGGGTG 
CTGATAGCCA GCTCCAATCT ACTGGAACCC AGTGGAATCA CGATTGACTA CTTAACAGAC 
ACTTTGTACT GGTGTGACAC CAAGAGGTCT GTGATTGAAA TGGCCAATCT GGATGGCTCC 
AAACGCCGAA GACTTATCCA GAACGACGTA GGTCACCCCT TCTCTCTAGC CGTGTTTGAG 
GATCACCTGT GGGTCTCGGA TTGGGCTATC CCATCGGTAA TAAGGGTGAA CAAGAGGACT 
GGCCAAAACA GGGTACGTCT TCAAGGCAGC ATGCTGAAGC CCTCGTCACT GGTTGTGGTC 
CATCCATTGG CAAAACCAGG TGCAGATCCC TGCTTATACA GGAATGGAGG CTGTGAACAC 
ATCTGCCAAG AGAGCCTGGG CACAGCTCGG TGTTTGTGTC GTGAAGGTTT TGTGAAGGCC 
TGGGATGGGA AAATGTGTCT CCCTCAGGAT TATCCAATCC TGTCAGGTGA AAATGCTGAT 
CTTAGTAAAG AGGTGACATC ACTGAGCAAC TCCACTCAGG CTGAAGTACC AGACGATGAT 
GGGACAGAAT CTTCCACACT AGTGGCTGAA ATCATGGTGT CAGGCATGAA CTATGAAGAT 
GACTGTGGTC CCGGGGGGTG TGGAAGCCAT GCTCGATGCG TTTCAGACGG AGAGACTGCT 
GAGTGTCAGT GTCTGAAAGG GTTTGCCAGG GATGGAAACC TGTGTTCTGA TATAGATGAG 
TGTGTGCTGG CTAGATCGGA CTGCCCCAGC ACCTCGTCCA GGTGCATCAA CACTGAAGGT 
GGCTACGTCT GCAGATGCTC AGAAGGCTAC GAAGGAGACG GGATCTCCTG TTTCGACTCT 
ACCGCACCCT CTCTCCTTGG GGAAGATGGC CACCATTTGG ACCGAAATAG TTATCCAGGA 
TGCCCATCCT CATATGATGG ATACTGCCTC AATGGTGGCG TGTGCATGCA TATTGAATCA 
CTGGACAGCT ACACATGCAA CTGTGTTATT GGCTATTCTG GGGATCGATG CCAGACTCGA 
GACCTACGAT GGTGGGAACT GCGTCATGCT GGCTACGGGC AGAAGCATGA CATCATGGTG 
GTGGCTGTCT GCATGGTGGC ACTGGTCCTG CTGCTCGTCT TGGGGATGTG GGGGACTTAC 
TACTACAGGA CTCGGAAGCA GCTATCAAAC CCCCCAAAGA ACCCTTGTGA TGAGCCAAGC 
GGAAGTGTGA GCAGCAGCGG ACCCAACAGC AGCAGCGGGG CAGCTGTGGC TTCTTGTCCC 
CAACCTTGGT TTGTGGTCCT AGAGAAACAC CAAGACCCCA AGAATGGGAG TCTGCCTGCG 
GATGGTACGA ATGGTGCAGT AGTAGATGCT GGCCTGTCTC CCTCCCTGCA GCTCGGGTCA 
GTGCATCTGA CTTCATGGAG ACAGAAGCCC CACATAGATG GAATGGGCAC AGGGCAAAGC 
TGCTGGATTC CACCATCAAG TGACAGAGGA CCCCAGGAAA TAGAGGGAAA CTCCCACCTA 
CCCTCCTACA GACCTGTGGG GCCGGAGAAG CTGCATTCTC TCCAGTCAGC TAATGGATCG 
TGTCACGAAA GGGCTCCAGA CCTGCCACGG CAGACAGAGC CAGTTCAGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Egf ( XM_011240010.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011238312.1 Mus musculus epidermal growth factor (Egf), transcript variant X1, mRNA.

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CloneID	OMu67604
Clone ID Related Accession (Same CDS sequence)	XM_011240010.2 , XM_011240010.3
Accession Version	XM_011240010.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3666bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	pro-epidermal growth factor isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000069.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_011240010.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCCTGGG GCCGAAGGCC AACCTGGCTG TTGCTCGCCT TCCTGCTGGT GTTTTTAAAG 
ATTAGCATAC TCAGCGTCAC AGCATGGCAG ACCGGGAACT GTCAGCCAGG TCCTCTCGAG 
AGAAGCGAGA GAAGCGGGAC TTGTGCCGGT CCTGCCCCCT TCCTAGTTTT CTCACAAGGA 
AAGAGCATCT CTCGGATTGA CCCAGATGGA ACAAATCACC AGCAATTGGT GGTGGATGCT 
GGCATCTCAG CAGACATGGA TATTCATTAT AAAAAAGAGA GACTCTATTG GGTGGATGTA 
GAAAGACAAG TTTTGCTAAG AGTTTTCCTT AACGGGACAG GACTAGAGAA AGTGTGCAAT 
GTAGAGAGGA AGGTGTCTGG GCTGGCCATA GACTGGATAG ATGATGAAGT TCTCTGGGTA 
GACCAACAGA ACGGAGTCAT CACCGTAACA GATATGACAG GGAAAAATTC CCGAGTTCTT 
CTAAGTTCCT TAAAACATCC GTCAAATATA GCAGTGGATC CAATAGAGAG GTTGATGTTT 
TGGTCTTCAG AGGTGACCGG CAGCCTTCAC AGAGCACACC TCAAAGGTGT TGATGTAAAA 
ACACTGCTGG AGACAGGGGG AATATCGGTG CTGACTCTGG ATGTCCTGGA CAAACGGCTC 
TTCTGGGTTC AGGACAGTGG CGAAGGAAGC CACGCTTACA TTCATTCCTG TGATTATGAG 
GGTGGCTCCG TCCGTCTTAT CAGGCATCAA GCACGGCACA GTTTGTCTTC AATGGCCTTT 
TTTGGTGATC GGATCTTCTA CTCAGTGTTG AAAAGCAAGG CGATTTGGAT AGCCAACAAA 
CACACGGGGA AGGACACGGT CAGGATTAAC CTCCATCCAT CCTTTGTGAC ACCTGGAAAA 
CTGATGGTAG TACACCCTCG TGCACAGCCC AGGACAGAGG ACGCTGCTAA GGATCCTGGT 
GAGTCACTAG ACCCCGAACT TCTCAAACAG AGGGGAAGAC CATGCCGCTT CGGTCTCTGT 
GAGCGAGACC CCAAGTCCCA CTCGAGCGCA TGCGCTGAGG GCTACACGTT AAGCCGAGAC 
CGGAAGTACT GCGAAGATGT CAATGAATGT GCCACTCAGA ATCACGGCTG TACTCTTGGG 
TGTGAAAACA CCCCTGGATC CTATCACTGC ACATGCCCCA CAGGATTTGT TCTGCTTCCT 
GATGGGAAAC AATGTCACGA ACTTGTTTCC TGCCCAGGCA ACGTATCAAA GTGCAGTCAT 
GGCTGTGTCC TGACATCAGA TGGTCCCCGG TGCATCTGTC CTGCAGGTTC AGTGCTTGGG 
AGAGATGGGA AGACTTGCAC TGGTTGTTCA TCGCCTGACA ATGGTGGATG CAGCCAGATC 
TGTCTTCCTC TCAGGCCAGG ATCCTGGGAA TGTGATTGCT TTCCTGGGTA TGACCTACAG 
TCAGACCGAA AGAGCTGTGC AGCTTCAGGA CCACAGCCAC TTTTACTGTT TGCAAATTCC 
CAGGACATCC GACACATGCA TTTTGATGGA ACAGACTACA AAGTTCTGCT CAGCCGGCAG 
ATGGGAATGG TTTTTGCCTT GGATTATGAC CCTGTGGAAA GCAAGATATA TTTTGCACAG 
ACAGCCCTGA AGTGGATAGA GAGGGCTAAT ATGGATGGGT CCCAGCGAGA AAGACTGATC 
ACAGAAGGAG TAGATACGCT TGAAGGTCTT GCCCTGGACT GGATTGGCCG GAGAATCTAC 
TGGACAGACA GTGGGAAGTC TGTTGTTGGA GGGAGCGATC TGAGCGGGAA GCATCATCGA 
ATAATCATCC AGGAGAGAAT CTCGAGGCCG CGAGGAATAG CTGTGCATCC AAGGGCCAGG 
AGACTGTTCT GGACGGACGT AGGGATGTCT CCACGGATTG AAAGCGCTTC CCTTCAAGGT 
TCCGACCGGG TGCTGATAGC CAGCTCCAAT CTACTGGAAC CCAGTGGAAT CACGATTGAC 
TACTTAACAG ACACTTTGTA CTGGTGTGAC ACCAAGAGGT CTGTGATTGA AATGGCCAAT 
CTGGATGGCT CCAAACGCCG AAGACTTATC CAGAACGACG TAGGTCACCC CTTCTCTCTA 
GCCGTGTTTG AGGATCACCT GTGGGTCTCG GATTGGGCTA TCCCATCGGT AATAAGGGTG 
AACAAGAGGA CTGGCCAAAA CAGGGTACGT CTTCAAGGCA GCATGCTGAA GCCCTCGTCA 
CTGGTTGTGG TCCATCCATT GGCAAAACCA GGTGCAGATC CCTGCTTATA CAGGAATGGA 
GGCTGTGAAC ACATCTGCCA AGAGAGCCTG GGCACAGCTC GGTGTTTGTG TCGTGAAGGT 
TTTGTGAAGG CCTGGGATGG GAAAATGTGT CTCCCTCAGG ATTATCCAAT CCTGTCAGGT 
GAAAATGCTG ATCTTAGTAA AGAGGTGACA TCACTGAGCA ACTCCACTCA GGCTGAAGTA 
CCAGACGATG ATGGGACAGA ATCTTCCACA CTAGTGGCTG AAATCATGGT GTCAGGCATG 
AACTATGAAG ATGACTGTGG TCCCGGGGGG TGTGGAAGCC ATGCTCGATG CGTTTCAGAC 
GGAGAGACTG CTGAGTGTCA GTGTCTGAAA GGGTTTGCCA GGGATGGAAA CCTGTGTTCT 
GATATAGATG AGTGTGTGCT GGCTAGATCG GACTGCCCCA GCACCTCGTC CAGGTGCATC 
AACACTGAAG GTGGCTACGT CTGCAGATGC TCAGAAGGCT ACGAAGGAGA CGGGATCTCC 
TGTTTCGATA TTGACGAGTG CCAGCGGGGG GCGCACAACT GCGGTGAGAA TGCCGCCTGC 
ACCAACACGG AGGGAGGCTA CAACTGCACC TGCGCAGGCC GCCCATCCTC GCCCGGACTG 
AGTTGCCCTG ACTCTACCGC ACCCTCTCTC CTTGGGGAAG ATGGCCACCA TTTGGACCGA 
AATAGTTATC CAGGATGCCC ATCCTCATAT GATGGATACT GCCTCAATGG TGGCGTGTGC 
ATGCATATTG AATCACTGGA CAGCTACACA TGCAACTGTG TTATTGGCTA TTCTGGGGAT 
CGATGCCAGA CTCGAGACCT ACGATGGTGG GAACTGCGTC ATGCTGGCTA CGGGCAGAAG 
CATGACATCA TGGTGGTGGC TGTCTGCATG GTGGCACTGG TCCTGCTGCT CGTCTTGGGG 
ATGTGGGGGA CTTACTACTA CAGGACTCGG AAGCAGCTAT CAAACCCCCC AAAGAACCCT 
TGTGATGAGC CAAGCGGAAG TGTGAGCAGC AGCGGACCCA ACAGCAGCAG CGGGGCAGCT 
GTGGCTTCTT GTCCCCAACC TTGGTTTGTG GTCCTAGAGA AACACCAAGA CCCCAAGAAT 
GGGAGTCTGC CTGCGGATGG TACGAATGGT GCAGTAGTAG ATGCTGGCCT GTCTCCCTCC 
CTGCAGCTCG GGTCAGTGCA TCTGACTTCA TGGAGACAGA AGCCCCACAT AGATGGAATG 
GGCACAGGGC AAAGCTGCTG GATTCCACCA TCAAGTGACA GAGGACCCCA GGAAATAGAG 
GGAAACTCCC ACCTACCCTC CTACAGACCT GTGGGGCCGG AGAAGCTGCA TTCTCTCCAG 
TCAGCTAATG GATCGTGTCA CGAAAGGGCT CCAGACCTGC CACGGCAGAC AGAGCCAGTT 
CAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011238312.1
CDS	135..3800
Translation	MPWGRRPTWLLLAFLLVFLKISILSVTAWQTGNCQPGPLERSERSGTCAGPAPFLVFSQGKSISRIDPDGTNHQQLVVDAGISADMDIHYKKERLYWVDVERQVLLRVFLNGTGLEKVCNVERKVSGLAIDWIDDEVLWVDQQNGVITVTDMTGKNSRVLLSSLKHPSNIAVDPIERLMFWSSEVTGSLHRAHLKGVDVKTLLETGGISVLTLDVLDKRLFWVQDSGEGSHAYIHSCDYEGGSVRLIRHQARHSLSSMAFFGDRIFYSVLKSKAIWIANKHTGKDTVRINLHPSFVTPGKLMVVHPRAQPRTEDAAKDPGESLDPELLKQRGRPCRFGLCERDPKSHSSACAEGYTLSRDRKYCEDVNECATQNHGCTLGCENTPGSYHCTCPTGFVLLPDGKQCHELVSCPGNVSKCSHGCVLTSDGPRCICPAGSVLGRDGKTCTGCSSPDNGGCSQICLPLRPGSWECDCFPGYDLQSDRKSCAASGPQPLLLFANSQDIRHMHFDGTDYKVLLSRQMGMVFALDYDPVESKIYFAQTALKWIERANMDGSQRERLITEGVDTLEGLALDWIGRRIYWTDSGKSVVGGSDLSGKHHRIIIQERISRPRGIAVHPRARRLFWTDVGMSPRIESASLQGSDRVLIASSNLLEPSGITIDYLTDTLYWCDTKRSVIEMANLDGSKRRRLIQNDVGHPFSLAVFEDHLWVSDWAIPSVIRVNKRTGQNRVRLQGSMLKPSSLVVVHPLAKPGADPCLYRNGGCEHICQESLGTARCLCREGFVKAWDGKMCLPQDYPILSGENADLSKEVTSLSNSTQAEVPDDDGTESSTLVAEIMVSGMNYEDDCGPGGCGSHARCVSDGETAECQCLKGFARDGNLCSDIDECVLARSDCPSTSSRCINTEGGYVCRCSEGYEGDGISCFDIDECQRGAHNCGENAACTNTEGGYNCTCAGRPSSPGLSCPDSTAPSLLGEDGHHLDRNSYPGCPSSYDGYCLNGGVCMHIESLDSYTCNCVIGYSGDRCQTRDLRWWELRHAGYGQKHDIMVVAVCMVALVLLLVLGMWGTYYYRTRKQLSNPPKNPCDEPSGSVSSSGPNSSSGAAVASCPQPWFVVLEKHQDPKNGSLPADGTNGAVVDAGLSPSLQLGSVHLTSWRQKPHIDGMGTGQSCWIPPSSDRGPQEIEGNSHLPSYRPVGPEKLHSLQSANGSCHERAPDLPRQTEPVQ

Target ORF information:

RefSeq Version	XM_011240010.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus epidermal growth factor (Egf), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011240010.3

ORF Insert Sequence:

ATGCCCTGGG GCCGAAGGCC AACCTGGCTG TTGCTCGCCT TCCTGCTGGT GTTTTTAAAG 
ATTAGCATAC TCAGCGTCAC AGCATGGCAG ACCGGGAACT GTCAGCCAGG TCCTCTCGAG 
AGAAGCGAGA GAAGCGGGAC TTGTGCCGGT CCTGCCCCCT TCCTAGTTTT CTCACAAGGA 
AAGAGCATCT CTCGGATTGA CCCAGATGGA ACAAATCACC AGCAATTGGT GGTGGATGCT 
GGCATCTCAG CAGACATGGA TATTCATTAT AAAAAAGAGA GACTCTATTG GGTGGATGTA 
GAAAGACAAG TTTTGCTAAG AGTTTTCCTT AACGGGACAG GACTAGAGAA AGTGTGCAAT 
GTAGAGAGGA AGGTGTCTGG GCTGGCCATA GACTGGATAG ATGATGAAGT TCTCTGGGTA 
GACCAACAGA ACGGAGTCAT CACCGTAACA GATATGACAG GGAAAAATTC CCGAGTTCTT 
CTAAGTTCCT TAAAACATCC GTCAAATATA GCAGTGGATC CAATAGAGAG GTTGATGTTT 
TGGTCTTCAG AGGTGACCGG CAGCCTTCAC AGAGCACACC TCAAAGGTGT TGATGTAAAA 
ACACTGCTGG AGACAGGGGG AATATCGGTG CTGACTCTGG ATGTCCTGGA CAAACGGCTC 
TTCTGGGTTC AGGACAGTGG CGAAGGAAGC CACGCTTACA TTCATTCCTG TGATTATGAG 
GGTGGCTCCG TCCGTCTTAT CAGGCATCAA GCACGGCACA GTTTGTCTTC AATGGCCTTT 
TTTGGTGATC GGATCTTCTA CTCAGTGTTG AAAAGCAAGG CGATTTGGAT AGCCAACAAA 
CACACGGGGA AGGACACGGT CAGGATTAAC CTCCATCCAT CCTTTGTGAC ACCTGGAAAA 
CTGATGGTAG TACACCCTCG TGCACAGCCC AGGACAGAGG ACGCTGCTAA GGATCCTGGT 
GAGTCACTAG ACCCCGAACT TCTCAAACAG AGGGGAAGAC CATGCCGCTT CGGTCTCTGT 
GAGCGAGACC CCAAGTCCCA CTCGAGCGCA TGCGCTGAGG GCTACACGTT AAGCCGAGAC 
CGGAAGTACT GCGAAGATGT CAATGAATGT GCCACTCAGA ATCACGGCTG TACTCTTGGG 
TGTGAAAACA CCCCTGGATC CTATCACTGC ACATGCCCCA CAGGATTTGT TCTGCTTCCT 
GATGGGAAAC AATGTCACGA ACTTGTTTCC TGCCCAGGCA ACGTATCAAA GTGCAGTCAT 
GGCTGTGTCC TGACATCAGA TGGTCCCCGG TGCATCTGTC CTGCAGGTTC AGTGCTTGGG 
AGAGATGGGA AGACTTGCAC TGGTTGTTCA TCGCCTGACA ATGGTGGATG CAGCCAGATC 
TGTCTTCCTC TCAGGCCAGG ATCCTGGGAA TGTGATTGCT TTCCTGGGTA TGACCTACAG 
TCAGACCGAA AGAGCTGTGC AGCTTCAGGA CCACAGCCAC TTTTACTGTT TGCAAATTCC 
CAGGACATCC GACACATGCA TTTTGATGGA ACAGACTACA AAGTTCTGCT CAGCCGGCAG 
ATGGGAATGG TTTTTGCCTT GGATTATGAC CCTGTGGAAA GCAAGATATA TTTTGCACAG 
ACAGCCCTGA AGTGGATAGA GAGGGCTAAT ATGGATGGGT CCCAGCGAGA AAGACTGATC 
ACAGAAGGAG TAGATACGCT TGAAGGTCTT GCCCTGGACT GGATTGGCCG GAGAATCTAC 
TGGACAGACA GTGGGAAGTC TGTTGTTGGA GGGAGCGATC TGAGCGGGAA GCATCATCGA 
ATAATCATCC AGGAGAGAAT CTCGAGGCCG CGAGGAATAG CTGTGCATCC AAGGGCCAGG 
AGACTGTTCT GGACGGACGT AGGGATGTCT CCACGGATTG AAAGCGCTTC CCTTCAAGGT 
TCCGACCGGG TGCTGATAGC CAGCTCCAAT CTACTGGAAC CCAGTGGAAT CACGATTGAC 
TACTTAACAG ACACTTTGTA CTGGTGTGAC ACCAAGAGGT CTGTGATTGA AATGGCCAAT 
CTGGATGGCT CCAAACGCCG AAGACTTATC CAGAACGACG TAGGTCACCC CTTCTCTCTA 
GCCGTGTTTG AGGATCACCT GTGGGTCTCG GATTGGGCTA TCCCATCGGT AATAAGGGTG 
AACAAGAGGA CTGGCCAAAA CAGGGTACGT CTTCAAGGCA GCATGCTGAA GCCCTCGTCA 
CTGGTTGTGG TCCATCCATT GGCAAAACCA GGTGCAGATC CCTGCTTATA CAGGAATGGA 
GGCTGTGAAC ACATCTGCCA AGAGAGCCTG GGCACAGCTC GGTGTTTGTG TCGTGAAGGT 
TTTGTGAAGG CCTGGGATGG GAAAATGTGT CTCCCTCAGG ATTATCCAAT CCTGTCAGGT 
GAAAATGCTG ATCTTAGTAA AGAGGTGACA TCACTGAGCA ACTCCACTCA GGCTGAAGTA 
CCAGACGATG ATGGGACAGA ATCTTCCACA CTAGTGGCTG AAATCATGGT GTCAGGCATG 
AACTATGAAG ATGACTGTGG TCCCGGGGGG TGTGGAAGCC ATGCTCGATG CGTTTCAGAC 
GGAGAGACTG CTGAGTGTCA GTGTCTGAAA GGGTTTGCCA GGGATGGAAA CCTGTGTTCT 
GATATAGATG AGTGTGTGCT GGCTAGATCG GACTGCCCCA GCACCTCGTC CAGGTGCATC 
AACACTGAAG GTGGCTACGT CTGCAGATGC TCAGAAGGCT ACGAAGGAGA CGGGATCTCC 
TGTTTCGATA TTGACGAGTG CCAGCGGGGG GCGCACAACT GCGGTGAGAA TGCCGCCTGC 
ACCAACACGG AGGGAGGCTA CAACTGCACC TGCGCAGGCC GCCCATCCTC GCCCGGACTG 
AGTTGCCCTG ACTCTACCGC ACCCTCTCTC CTTGGGGAAG ATGGCCACCA TTTGGACCGA 
AATAGTTATC CAGGATGCCC ATCCTCATAT GATGGATACT GCCTCAATGG TGGCGTGTGC 
ATGCATATTG AATCACTGGA CAGCTACACA TGCAACTGTG TTATTGGCTA TTCTGGGGAT 
CGATGCCAGA CTCGAGACCT ACGATGGTGG GAACTGCGTC ATGCTGGCTA CGGGCAGAAG 
CATGACATCA TGGTGGTGGC TGTCTGCATG GTGGCACTGG TCCTGCTGCT CGTCTTGGGG 
ATGTGGGGGA CTTACTACTA CAGGACTCGG AAGCAGCTAT CAAACCCCCC AAAGAACCCT 
TGTGATGAGC CAAGCGGAAG TGTGAGCAGC AGCGGACCCA ACAGCAGCAG CGGGGCAGCT 
GTGGCTTCTT GTCCCCAACC TTGGTTTGTG GTCCTAGAGA AACACCAAGA CCCCAAGAAT 
GGGAGTCTGC CTGCGGATGG TACGAATGGT GCAGTAGTAG ATGCTGGCCT GTCTCCCTCC 
CTGCAGCTCG GGTCAGTGCA TCTGACTTCA TGGAGACAGA AGCCCCACAT AGATGGAATG 
GGCACAGGGC AAAGCTGCTG GATTCCACCA TCAAGTGACA GAGGACCCCA GGAAATAGAG 
GGAAACTCCC ACCTACCCTC CTACAGACCT GTGGGGCCGG AGAAGCTGCA TTCTCTCCAG 
TCAGCTAATG GATCGTGTCA CGAAAGGGCT CCAGACCTGC CACGGCAGAC AGAGCCAGTT 
CAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Egf ( XM_011240011.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011238313.1 Mus musculus epidermal growth factor (Egf), transcript variant X2, mRNA.

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CloneID	OMu67605
Clone ID Related Accession (Same CDS sequence)	XM_011240011.2 , XM_011240011.3
Accession Version	XM_011240011.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3543bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	pro-epidermal growth factor isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000069.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_011240011.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCCTGGG GCCGAAGGCC AACCTGGCTG TTGCTCGCCT TCCTGCTGGT GTTTTTAAAG 
ATTAGCATAC TCAGCGTCAC AGCATGGCAG ACCGGGAACT GTCAGCCAGG TCCTCTCGAG 
AGAAGCGAGA GAAGCGGGAC TTGTGCCGGT CCTGCCCCCT TCCTAGTTTT CTCACAAGGA 
AAGAGCATCT CTCGGATTGA CCCAGATGGA ACAAATCACC AGCAATTGGT GGTGGATGCT 
GGCATCTCAG CAGACATGGA TATTCATTAT AAAAAAGAGA GACTCTATTG GGTGGATGTA 
GAAAGACAAG TTTTGCTAAG AGTTTTCCTT AACGGGACAG GACTAGAGAA AGTGTGCAAT 
GTAGAGAGGA AGGTGTCTGG GCTGGCCATA GACTGGATAG ATGATGAAGT TCTCTGGGTA 
GACCAACAGA ACGGAGTCAT CACCGTAACA GATATGACAG GGAAAAATTC CCGAGTTCTT 
CTAAGTTCCT TAAAACATCC GTCAAATATA GCAGTGGATC CAATAGAGAG GTTGATGTTT 
TGGTCTTCAG AGGTGACCGG CAGCCTTCAC AGAGCACACC TCAAAGGTGT TGATGTAAAA 
ACACTGCTGG AGACAGGGGG AATATCGGTG CTGACTCTGG ATGTCCTGGA CAAACGGCTC 
TTCTGGGTTC AGGACAGTGG CGAAGGAAGC CACGCTTACA TTCATTCCTG TGATTATGAG 
GGTGGCTCCG TCCGTCTTAT CAGGCATCAA GCACGGCACA GTTTGTCTTC AATGGCCTTT 
TTTGGTGATC GGATCTTCTA CTCAGTGTTG AAAAGCAAGG CGATTTGGAT AGCCAACAAA 
CACACGGGGA AGGACACGGT CAGGATTAAC CTCCATCCAT CCTTTGTGAC ACCTGGAAAA 
CTGATGGTAG TACACCCTCG TGCACAGCCC AGGACAGAGG ACGCTGCTAA GGATCCTGGT 
GAGTCACTAG ACCCCGAACT TCTCAAACAG AGGGGAAGAC CATGCCGCTT CGGTCTCTGT 
GAGCGAGACC CCAAGTCCCA CTCGAGCGCA TGCGCTGAGG GCTACACGTT AAGCCGAGAC 
CGGAAGTACT GCGAAGATGT CAATGAATGT GCCACTCAGA ATCACGGCTG TACTCTTGGG 
TGTGAAAACA CCCCTGGATC CTATCACTGC ACATGCCCCA CAGGATTTGT TCTGCTTCCT 
GATGGGAAAC AATGTCACGA ACTTGTTTCC TGCCCAGGCA ACGTATCAAA GTGCAGTCAT 
GGCTGTGTCC TGACATCAGA TGGTCCCCGG TGCATCTGTC CTGCAGGTTC AGTGCTTGGG 
AGAGATGGGA AGACTTGCAC TGGTTGTTCA TCGCCTGACA ATGGTGGATG CAGCCAGATC 
TGTCTTCCTC TCAGGCCAGG ATCCTGGGAA TGTGATTGCT TTCCTGGGTA TGACCTACAG 
TCAGACCGAA AGAGCTGTGC AGCTTCAGGA CCACAGCCAC TTTTACTGTT TGCAAATTCC 
CAGGACATCC GACACATGCA TTTTGATGGA ACAGACTACA AAGTTCTGCT CAGCCGGCAG 
ATGGGAATGG TTTTTGCCTT GGATTATGAC CCTGTGGAAA GCAAGATATA TTTTGCACAG 
ACAGCCCTGA AGTGGATAGA GAGGGCTAAT ATGGATGGGT CCCAGCGAGA AAGACTGATC 
ACAGAAGGAG TAGATACGCT TGAAGGTCTT GCCCTGGACT GGATTGGCCG GAGAATCTAC 
TGGACAGACA GTGGGAAGTC TGTTGTTGGA GGGAGCGATC TGAGCGGGAA GCATCATCGA 
ATAATCATCC AGGAGAGAAT CTCGAGGCCG CGAGGAATAG CTGTGCATCC AAGGGCCAGG 
AGACTGTTCT GGACGGACGT AGGGATGTCT CCACGGATTG AAAGCGCTTC CCTTCAAGGT 
TCCGACCGGG TGCTGATAGC CAGCTCCAAT CTACTGGAAC CCAGTGGAAT CACGATTGAC 
TACTTAACAG ACACTTTGTA CTGGTGTGAC ACCAAGAGGT CTGTGATTGA AATGGCCAAT 
CTGGATGGCT CCAAACGCCG AAGACTTATC CAGAACGACG TAGGTCACCC CTTCTCTCTA 
GCCGTGTTTG AGGATCACCT GTGGGTCTCG GATTGGGCTA TCCCATCGGT AATAAGGGTG 
AACAAGAGGA CTGGCCAAAA CAGGGTACGT CTTCAAGGCA GCATGCTGAA GCCCTCGTCA 
CTGGTTGTGG TCCATCCATT GGCAAAACCA GGTGCAGATC CCTGCTTATA CAGGAATGGA 
GGCTGTGAAC ACATCTGCCA AGAGAGCCTG GGCACAGCTC GGTGTTTGTG TCGTGAAGGT 
TTTGTGAAGG CCTGGGATGG GAAAATGTGT CTCCCTCAGG ATTATCCAAT CCTGTCAGGT 
GAAAATGCTG ATCTTAGTAA AGAGGTGACA TCACTGAGCA ACTCCACTCA GGCTGAAGTA 
CCAGACGATG ATGGGACAGA ATCTTCCACA CTAGTGGCTG AAATCATGGT GTCAGGCATG 
AACTATGAAG ATGACTGTGG TCCCGGGGGG TGTGGAAGCC ATGCTCGATG CGTTTCAGAC 
GGAGAGACTG CTGAGTGTCA GTGTCTGAAA GGGTTTGCCA GGGATGGAAA CCTGTGTTCT 
GATATAGATG AGTGTGTGCT GGCTAGATCG GACTGCCCCA GCACCTCGTC CAGGTGCATC 
AACACTGAAG GTGGCTACGT CTGCAGATGC TCAGAAGGCT ACGAAGGAGA CGGGATCTCC 
TGTTTCGACT CTACCGCACC CTCTCTCCTT GGGGAAGATG GCCACCATTT GGACCGAAAT 
AGTTATCCAG GATGCCCATC CTCATATGAT GGATACTGCC TCAATGGTGG CGTGTGCATG 
CATATTGAAT CACTGGACAG CTACACATGC AACTGTGTTA TTGGCTATTC TGGGGATCGA 
TGCCAGACTC GAGACCTACG ATGGTGGGAA CTGCGTCATG CTGGCTACGG GCAGAAGCAT 
GACATCATGG TGGTGGCTGT CTGCATGGTG GCACTGGTCC TGCTGCTCGT CTTGGGGATG 
TGGGGGACTT ACTACTACAG GACTCGGAAG CAGCTATCAA ACCCCCCAAA GAACCCTTGT 
GATGAGCCAA GCGGAAGTGT GAGCAGCAGC GGACCCAACA GCAGCAGCGG GGCAGCTGTG 
GCTTCTTGTC CCCAACCTTG GTTTGTGGTC CTAGAGAAAC ACCAAGACCC CAAGAATGGG 
AGTCTGCCTG CGGATGGTAC GAATGGTGCA GTAGTAGATG CTGGCCTGTC TCCCTCCCTG 
CAGCTCGGGT CAGTGCATCT GACTTCATGG AGACAGAAGC CCCACATAGA TGGAATGGGC 
ACAGGGCAAA GCTGCTGGAT TCCACCATCA AGTGACAGAG GACCCCAGGA AATAGAGGGA 
AACTCCCACC TACCCTCCTA CAGACCTGTG GGGCCGGAGA AGCTGCATTC TCTCCAGTCA 
GCTAATGGAT CGTGTCACGA AAGGGCTCCA GACCTGCCAC GGCAGACAGA GCCAGTTCAG 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011238313.1
CDS	135..3677
Translation	MPWGRRPTWLLLAFLLVFLKISILSVTAWQTGNCQPGPLERSERSGTCAGPAPFLVFSQGKSISRIDPDGTNHQQLVVDAGISADMDIHYKKERLYWVDVERQVLLRVFLNGTGLEKVCNVERKVSGLAIDWIDDEVLWVDQQNGVITVTDMTGKNSRVLLSSLKHPSNIAVDPIERLMFWSSEVTGSLHRAHLKGVDVKTLLETGGISVLTLDVLDKRLFWVQDSGEGSHAYIHSCDYEGGSVRLIRHQARHSLSSMAFFGDRIFYSVLKSKAIWIANKHTGKDTVRINLHPSFVTPGKLMVVHPRAQPRTEDAAKDPGESLDPELLKQRGRPCRFGLCERDPKSHSSACAEGYTLSRDRKYCEDVNECATQNHGCTLGCENTPGSYHCTCPTGFVLLPDGKQCHELVSCPGNVSKCSHGCVLTSDGPRCICPAGSVLGRDGKTCTGCSSPDNGGCSQICLPLRPGSWECDCFPGYDLQSDRKSCAASGPQPLLLFANSQDIRHMHFDGTDYKVLLSRQMGMVFALDYDPVESKIYFAQTALKWIERANMDGSQRERLITEGVDTLEGLALDWIGRRIYWTDSGKSVVGGSDLSGKHHRIIIQERISRPRGIAVHPRARRLFWTDVGMSPRIESASLQGSDRVLIASSNLLEPSGITIDYLTDTLYWCDTKRSVIEMANLDGSKRRRLIQNDVGHPFSLAVFEDHLWVSDWAIPSVIRVNKRTGQNRVRLQGSMLKPSSLVVVHPLAKPGADPCLYRNGGCEHICQESLGTARCLCREGFVKAWDGKMCLPQDYPILSGENADLSKEVTSLSNSTQAEVPDDDGTESSTLVAEIMVSGMNYEDDCGPGGCGSHARCVSDGETAECQCLKGFARDGNLCSDIDECVLARSDCPSTSSRCINTEGGYVCRCSEGYEGDGISCFDSTAPSLLGEDGHHLDRNSYPGCPSSYDGYCLNGGVCMHIESLDSYTCNCVIGYSGDRCQTRDLRWWELRHAGYGQKHDIMVVAVCMVALVLLLVLGMWGTYYYRTRKQLSNPPKNPCDEPSGSVSSSGPNSSSGAAVASCPQPWFVVLEKHQDPKNGSLPADGTNGAVVDAGLSPSLQLGSVHLTSWRQKPHIDGMGTGQSCWIPPSSDRGPQEIEGNSHLPSYRPVGPEKLHSLQSANGSCHERAPDLPRQTEPVQ

Target ORF information:

RefSeq Version	XM_011240011.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus epidermal growth factor (Egf), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011240011.3

ORF Insert Sequence:

ATGCCCTGGG GCCGAAGGCC AACCTGGCTG TTGCTCGCCT TCCTGCTGGT GTTTTTAAAG 
ATTAGCATAC TCAGCGTCAC AGCATGGCAG ACCGGGAACT GTCAGCCAGG TCCTCTCGAG 
AGAAGCGAGA GAAGCGGGAC TTGTGCCGGT CCTGCCCCCT TCCTAGTTTT CTCACAAGGA 
AAGAGCATCT CTCGGATTGA CCCAGATGGA ACAAATCACC AGCAATTGGT GGTGGATGCT 
GGCATCTCAG CAGACATGGA TATTCATTAT AAAAAAGAGA GACTCTATTG GGTGGATGTA 
GAAAGACAAG TTTTGCTAAG AGTTTTCCTT AACGGGACAG GACTAGAGAA AGTGTGCAAT 
GTAGAGAGGA AGGTGTCTGG GCTGGCCATA GACTGGATAG ATGATGAAGT TCTCTGGGTA 
GACCAACAGA ACGGAGTCAT CACCGTAACA GATATGACAG GGAAAAATTC CCGAGTTCTT 
CTAAGTTCCT TAAAACATCC GTCAAATATA GCAGTGGATC CAATAGAGAG GTTGATGTTT 
TGGTCTTCAG AGGTGACCGG CAGCCTTCAC AGAGCACACC TCAAAGGTGT TGATGTAAAA 
ACACTGCTGG AGACAGGGGG AATATCGGTG CTGACTCTGG ATGTCCTGGA CAAACGGCTC 
TTCTGGGTTC AGGACAGTGG CGAAGGAAGC CACGCTTACA TTCATTCCTG TGATTATGAG 
GGTGGCTCCG TCCGTCTTAT CAGGCATCAA GCACGGCACA GTTTGTCTTC AATGGCCTTT 
TTTGGTGATC GGATCTTCTA CTCAGTGTTG AAAAGCAAGG CGATTTGGAT AGCCAACAAA 
CACACGGGGA AGGACACGGT CAGGATTAAC CTCCATCCAT CCTTTGTGAC ACCTGGAAAA 
CTGATGGTAG TACACCCTCG TGCACAGCCC AGGACAGAGG ACGCTGCTAA GGATCCTGGT 
GAGTCACTAG ACCCCGAACT TCTCAAACAG AGGGGAAGAC CATGCCGCTT CGGTCTCTGT 
GAGCGAGACC CCAAGTCCCA CTCGAGCGCA TGCGCTGAGG GCTACACGTT AAGCCGAGAC 
CGGAAGTACT GCGAAGATGT CAATGAATGT GCCACTCAGA ATCACGGCTG TACTCTTGGG 
TGTGAAAACA CCCCTGGATC CTATCACTGC ACATGCCCCA CAGGATTTGT TCTGCTTCCT 
GATGGGAAAC AATGTCACGA ACTTGTTTCC TGCCCAGGCA ACGTATCAAA GTGCAGTCAT 
GGCTGTGTCC TGACATCAGA TGGTCCCCGG TGCATCTGTC CTGCAGGTTC AGTGCTTGGG 
AGAGATGGGA AGACTTGCAC TGGTTGTTCA TCGCCTGACA ATGGTGGATG CAGCCAGATC 
TGTCTTCCTC TCAGGCCAGG ATCCTGGGAA TGTGATTGCT TTCCTGGGTA TGACCTACAG 
TCAGACCGAA AGAGCTGTGC AGCTTCAGGA CCACAGCCAC TTTTACTGTT TGCAAATTCC 
CAGGACATCC GACACATGCA TTTTGATGGA ACAGACTACA AAGTTCTGCT CAGCCGGCAG 
ATGGGAATGG TTTTTGCCTT GGATTATGAC CCTGTGGAAA GCAAGATATA TTTTGCACAG 
ACAGCCCTGA AGTGGATAGA GAGGGCTAAT ATGGATGGGT CCCAGCGAGA AAGACTGATC 
ACAGAAGGAG TAGATACGCT TGAAGGTCTT GCCCTGGACT GGATTGGCCG GAGAATCTAC 
TGGACAGACA GTGGGAAGTC TGTTGTTGGA GGGAGCGATC TGAGCGGGAA GCATCATCGA 
ATAATCATCC AGGAGAGAAT CTCGAGGCCG CGAGGAATAG CTGTGCATCC AAGGGCCAGG 
AGACTGTTCT GGACGGACGT AGGGATGTCT CCACGGATTG AAAGCGCTTC CCTTCAAGGT 
TCCGACCGGG TGCTGATAGC CAGCTCCAAT CTACTGGAAC CCAGTGGAAT CACGATTGAC 
TACTTAACAG ACACTTTGTA CTGGTGTGAC ACCAAGAGGT CTGTGATTGA AATGGCCAAT 
CTGGATGGCT CCAAACGCCG AAGACTTATC CAGAACGACG TAGGTCACCC CTTCTCTCTA 
GCCGTGTTTG AGGATCACCT GTGGGTCTCG GATTGGGCTA TCCCATCGGT AATAAGGGTG 
AACAAGAGGA CTGGCCAAAA CAGGGTACGT CTTCAAGGCA GCATGCTGAA GCCCTCGTCA 
CTGGTTGTGG TCCATCCATT GGCAAAACCA GGTGCAGATC CCTGCTTATA CAGGAATGGA 
GGCTGTGAAC ACATCTGCCA AGAGAGCCTG GGCACAGCTC GGTGTTTGTG TCGTGAAGGT 
TTTGTGAAGG CCTGGGATGG GAAAATGTGT CTCCCTCAGG ATTATCCAAT CCTGTCAGGT 
GAAAATGCTG ATCTTAGTAA AGAGGTGACA TCACTGAGCA ACTCCACTCA GGCTGAAGTA 
CCAGACGATG ATGGGACAGA ATCTTCCACA CTAGTGGCTG AAATCATGGT GTCAGGCATG 
AACTATGAAG ATGACTGTGG TCCCGGGGGG TGTGGAAGCC ATGCTCGATG CGTTTCAGAC 
GGAGAGACTG CTGAGTGTCA GTGTCTGAAA GGGTTTGCCA GGGATGGAAA CCTGTGTTCT 
GATATAGATG AGTGTGTGCT GGCTAGATCG GACTGCCCCA GCACCTCGTC CAGGTGCATC 
AACACTGAAG GTGGCTACGT CTGCAGATGC TCAGAAGGCT ACGAAGGAGA CGGGATCTCC 
TGTTTCGACT CTACCGCACC CTCTCTCCTT GGGGAAGATG GCCACCATTT GGACCGAAAT 
AGTTATCCAG GATGCCCATC CTCATATGAT GGATACTGCC TCAATGGTGG CGTGTGCATG 
CATATTGAAT CACTGGACAG CTACACATGC AACTGTGTTA TTGGCTATTC TGGGGATCGA 
TGCCAGACTC GAGACCTACG ATGGTGGGAA CTGCGTCATG CTGGCTACGG GCAGAAGCAT 
GACATCATGG TGGTGGCTGT CTGCATGGTG GCACTGGTCC TGCTGCTCGT CTTGGGGATG 
TGGGGGACTT ACTACTACAG GACTCGGAAG CAGCTATCAA ACCCCCCAAA GAACCCTTGT 
GATGAGCCAA GCGGAAGTGT GAGCAGCAGC GGACCCAACA GCAGCAGCGG GGCAGCTGTG 
GCTTCTTGTC CCCAACCTTG GTTTGTGGTC CTAGAGAAAC ACCAAGACCC CAAGAATGGG 
AGTCTGCCTG CGGATGGTAC GAATGGTGCA GTAGTAGATG CTGGCCTGTC TCCCTCCCTG 
CAGCTCGGGT CAGTGCATCT GACTTCATGG AGACAGAAGC CCCACATAGA TGGAATGGGC 
ACAGGGCAAA GCTGCTGGAT TCCACCATCA AGTGACAGAG GACCCCAGGA AATAGAGGGA 
AACTCCCACC TACCCTCCTA CAGACCTGTG GGGCCGGAGA AGCTGCATTC TCTCCAGTCA 
GCTAATGGAT CGTGTCACGA AAGGGCTCCA GACCTGCCAC GGCAGACAGA GCCAGTTCAG 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Egf ( NM_010113.4 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_034243.2 Mus musculus epidermal growth factor (Egf), transcript variant 1, mRNA.

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CloneID	OMu22638
Clone ID Related Accession (Same CDS sequence)	NM_010113.4
Accession Version	NM_010113.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3654bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-11
Organism	Mus musculus(house mouse)
Product	pro-epidermal growth factor isoform 1 preproprotein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC098732.3, AK136439.1 and BP761200.1. On Jul 18, 2015 this sequence version replaced NM_010113.3. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC060741.1, J00380.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCCCTGGG GCCGAAGGCC AACCTGGCTG TTGCTCGCCT TCCTGCTGGT GTTTTTAAAG 
ATTAGCATAC TCAGCGTCAC AGCATGGCAG ACCGGGAACT GTCAGCCAGG TCCTCTCGAG 
AGAAGCGAGA GAAGCGGGAC TTGTGCCGGT CCTGCCCCCT TCCTAGTTTT CTCACAAGGA 
AAGAGCATCT CTCGGATTGA CCCAGATGGA ACAAATCACC AGCAATTGGT GGTGGATGCT 
GGCATCTCAG CAGACATGGA TATTCATTAT AAAAAAGAGA GACTCTATTG GGTGGATGTA 
GAAAGACAAG TTTTGCTAAG AGTTTTCCTT AACGGGACAG GACTAGAGAA AGTGTGCAAT 
GTAGAGAGGA AGGTGTCTGG GCTGGCCATA GACTGGATAG ATGATGAAGT TCTCTGGGTA 
GACCAACAGA ACGGAGTCAT CACCGTAACA GATATGACAG GGAAAAATTC CCGAGTTCTT 
CTAAGTTCCT TAAAACATCC GTCAAATATA GCAGTGGATC CAATAGAGAG GTTGATGTTT 
TGGTCTTCAG AGGTGACCGG CAGCCTTCAC AGAGCACACC TCAAAGGTGT TGATGTAAAA 
ACACTGCTGG AGACAGGGGG AATATCGGTG CTGACTCTGG ATGTCCTGGA CAAACGGCTC 
TTCTGGGTTC AGGACAGTGG CGAAGGAAGC CACGCTTACA TTCATTCCTG TGATTATGAG 
GGTGGCTCCG TCCGTCTTAT CAGGCATCAA GCACGGCACA GTTTGTCTTC AATGGCCTTT 
TTTGGTGATC GGATCTTCTA CTCAGTGTTG AAAAGCAAGG CGATTTGGAT AGCCAACAAA 
CACACGGGGA AGGACACGGT CAGGATTAAC CTCCATCCAT CCTTTGTGAC ACCTGGAAAA 
CTGATGGTAG TACACCCTCG TGCACAGCCC AGGACAGAGG ACGCTGCTAA GGATCCTGAC 
CCCGAACTTC TCAAACAGAG GGGAAGACCA TGCCGCTTCG GTCTCTGTGA GCGAGACCCC 
AAGTCCCACT CGAGCGCATG CGCTGAGGGC TACACGTTAA GCCGAGACCG GAAGTACTGC 
GAAGATGTCA ATGAATGTGC CACTCAGAAT CACGGCTGTA CTCTTGGGTG TGAAAACACC 
CCTGGATCCT ATCACTGCAC ATGCCCCACA GGATTTGTTC TGCTTCCTGA TGGGAAACAA 
TGTCACGAAC TTGTTTCCTG CCCAGGCAAC GTATCAAAGT GCAGTCATGG CTGTGTCCTG 
ACATCAGATG GTCCCCGGTG CATCTGTCCT GCAGGTTCAG TGCTTGGGAG AGATGGGAAG 
ACTTGCACTG GTTGTTCATC GCCTGACAAT GGTGGATGCA GCCAGATCTG TCTTCCTCTC 
AGGCCAGGAT CCTGGGAATG TGATTGCTTT CCTGGGTATG ACCTACAGTC AGACCGAAAG 
AGCTGTGCAG CTTCAGGACC ACAGCCACTT TTACTGTTTG CAAATTCCCA GGACATCCGA 
CACATGCATT TTGATGGAAC AGACTACAAA GTTCTGCTCA GCCGGCAGAT GGGAATGGTT 
TTTGCCTTGG ATTATGACCC TGTGGAAAGC AAGATATATT TTGCACAGAC AGCCCTGAAG 
TGGATAGAGA GGGCTAATAT GGATGGGTCC CAGCGAGAAA GACTGATCAC AGAAGGAGTA 
GATACGCTTG AAGGTCTTGC CCTGGACTGG ATTGGCCGGA GAATCTACTG GACAGACAGT 
GGGAAGTCTG TTGTTGGAGG GAGCGATCTG AGCGGGAAGC ATCATCGAAT AATCATCCAG 
GAGAGAATCT CGAGGCCGCG AGGAATAGCT GTGCATCCAA GGGCCAGGAG ACTGTTCTGG 
ACGGACGTAG GGATGTCTCC ACGGATTGAA AGCGCTTCCC TTCAAGGTTC CGACCGGGTG 
CTGATAGCCA GCTCCAATCT ACTGGAACCC AGTGGAATCA CGATTGACTA CTTAACAGAC 
ACTTTGTACT GGTGTGACAC CAAGAGGTCT GTGATTGAAA TGGCCAATCT GGATGGCTCC 
AAACGCCGAA GACTTATCCA GAACGACGTA GGTCACCCCT TCTCTCTAGC CGTGTTTGAG 
GATCACCTGT GGGTCTCGGA TTGGGCTATC CCATCGGTAA TAAGGGTGAA CAAGAGGACT 
GGCCAAAACA GGGTACGTCT TCAAGGCAGC ATGCTGAAGC CCTCGTCACT GGTTGTGGTC 
CATCCATTGG CAAAACCAGG TGCAGATCCC TGCTTATACA GGAATGGAGG CTGTGAACAC 
ATCTGCCAAG AGAGCCTGGG CACAGCTCGG TGTTTGTGTC GTGAAGGTTT TGTGAAGGCC 
TGGGATGGGA AAATGTGTCT CCCTCAGGAT TATCCAATCC TGTCAGGTGA AAATGCTGAT 
CTTAGTAAAG AGGTGACATC ACTGAGCAAC TCCACTCAGG CTGAAGTACC AGACGATGAT 
GGGACAGAAT CTTCCACACT AGTGGCTGAA ATCATGGTGT CAGGCATGAA CTATGAAGAT 
GACTGTGGTC CCGGGGGGTG TGGAAGCCAT GCTCGATGCG TTTCAGACGG AGAGACTGCT 
GAGTGTCAGT GTCTGAAAGG GTTTGCCAGG GATGGAAACC TGTGTTCTGA TATAGATGAG 
TGTGTGCTGG CTAGATCGGA CTGCCCCAGC ACCTCGTCCA GGTGCATCAA CACTGAAGGT 
GGCTACGTCT GCAGATGCTC AGAAGGCTAC GAAGGAGACG GGATCTCCTG TTTCGATATT 
GACGAGTGCC AGCGGGGGGC GCACAACTGC GGTGAGAATG CCGCCTGCAC CAACACGGAG 
GGAGGCTACA ACTGCACCTG CGCAGGCCGC CCATCCTCGC CCGGACTGAG TTGCCCTGAC 
TCTACCGCAC CCTCTCTCCT TGGGGAAGAT GGCCACCATT TGGACCGAAA TAGTTATCCA 
GGATGCCCAT CCTCATATGA TGGATACTGC CTCAATGGTG GCGTGTGCAT GCATATTGAA 
TCACTGGACA GCTACACATG CAACTGTGTT ATTGGCTATT CTGGGGATCG ATGCCAGACT 
CGAGACCTAC GATGGTGGGA ACTGCGTCAT GCTGGCTACG GGCAGAAGCA TGACATCATG 
GTGGTGGCTG TCTGCATGGT GGCACTGGTC CTGCTGCTCG TCTTGGGGAT GTGGGGGACT 
TACTACTACA GGACTCGGAA GCAGCTATCA AACCCCCCAA AGAACCCTTG TGATGAGCCA 
AGCGGAAGTG TGAGCAGCAG CGGACCCAAC AGCAGCAGCG GGGCAGCTGT GGCTTCTTGT 
CCCCAACCTT GGTTTGTGGT CCTAGAGAAA CACCAAGACC CCAAGAATGG GAGTCTGCCT 
GCGGATGGTA CGAATGGTGC AGTAGTAGAT GCTGGCCTGT CTCCCTCCCT GCAGCTCGGG 
TCAGTGCATC TGACTTCATG GAGACAGAAG CCCCACATAG ATGGAATGGG CACAGGGCAA 
AGCTGCTGGA TTCCACCATC AAGTGACAGA GGACCCCAGG AAATAGAGGG AAACTCCCAC 
CTACCCTCCT ACAGACCTGT GGGGCCGGAG AAGCTGCATT CTCTCCAGTC AGCTAATGGA 
TCGTGTCACG AAAGGGCTCC AGACCTGCCA CGGCAGACAG AGCCAGTTCA GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_034243.2
CDS	359..4012
Translation	MPWGRRPTWLLLAFLLVFLKISILSVTAWQTGNCQPGPLERSERSGTCAGPAPFLVFSQGKSISRIDPDGTNHQQLVVDAGISADMDIHYKKERLYWVDVERQVLLRVFLNGTGLEKVCNVERKVSGLAIDWIDDEVLWVDQQNGVITVTDMTGKNSRVLLSSLKHPSNIAVDPIERLMFWSSEVTGSLHRAHLKGVDVKTLLETGGISVLTLDVLDKRLFWVQDSGEGSHAYIHSCDYEGGSVRLIRHQARHSLSSMAFFGDRIFYSVLKSKAIWIANKHTGKDTVRINLHPSFVTPGKLMVVHPRAQPRTEDAAKDPDPELLKQRGRPCRFGLCERDPKSHSSACAEGYTLSRDRKYCEDVNECATQNHGCTLGCENTPGSYHCTCPTGFVLLPDGKQCHELVSCPGNVSKCSHGCVLTSDGPRCICPAGSVLGRDGKTCTGCSSPDNGGCSQICLPLRPGSWECDCFPGYDLQSDRKSCAASGPQPLLLFANSQDIRHMHFDGTDYKVLLSRQMGMVFALDYDPVESKIYFAQTALKWIERANMDGSQRERLITEGVDTLEGLALDWIGRRIYWTDSGKSVVGGSDLSGKHHRIIIQERISRPRGIAVHPRARRLFWTDVGMSPRIESASLQGSDRVLIASSNLLEPSGITIDYLTDTLYWCDTKRSVIEMANLDGSKRRRLIQNDVGHPFSLAVFEDHLWVSDWAIPSVIRVNKRTGQNRVRLQGSMLKPSSLVVVHPLAKPGADPCLYRNGGCEHICQESLGTARCLCREGFVKAWDGKMCLPQDYPILSGENADLSKEVTSLSNSTQAEVPDDDGTESSTLVAEIMVSGMNYEDDCGPGGCGSHARCVSDGETAECQCLKGFARDGNLCSDIDECVLARSDCPSTSSRCINTEGGYVCRCSEGYEGDGISCFDIDECQRGAHNCGENAACTNTEGGYNCTCAGRPSSPGLSCPDSTAPSLLGEDGHHLDRNSYPGCPSSYDGYCLNGGVCMHIESLDSYTCNCVIGYSGDRCQTRDLRWWELRHAGYGQKHDIMVVAVCMVALVLLLVLGMWGTYYYRTRKQLSNPPKNPCDEPSGSVSSSGPNSSSGAAVASCPQPWFVVLEKHQDPKNGSLPADGTNGAVVDAGLSPSLQLGSVHLTSWRQKPHIDGMGTGQSCWIPPSSDRGPQEIEGNSHLPSYRPVGPEKLHSLQSANGSCHERAPDLPRQTEPVQ

Target ORF information:

RefSeq Version	NM_010113.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus epidermal growth factor (Egf), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_010113.4

ORF Insert Sequence:

ATGCCCTGGG GCCGAAGGCC AACCTGGCTG TTGCTCGCCT TCCTGCTGGT GTTTTTAAAG 
ATTAGCATAC TCAGCGTCAC AGCATGGCAG ACCGGGAACT GTCAGCCAGG TCCTCTCGAG 
AGAAGCGAGA GAAGCGGGAC TTGTGCCGGT CCTGCCCCCT TCCTAGTTTT CTCACAAGGA 
AAGAGCATCT CTCGGATTGA CCCAGATGGA ACAAATCACC AGCAATTGGT GGTGGATGCT 
GGCATCTCAG CAGACATGGA TATTCATTAT AAAAAAGAGA GACTCTATTG GGTGGATGTA 
GAAAGACAAG TTTTGCTAAG AGTTTTCCTT AACGGGACAG GACTAGAGAA AGTGTGCAAT 
GTAGAGAGGA AGGTGTCTGG GCTGGCCATA GACTGGATAG ATGATGAAGT TCTCTGGGTA 
GACCAACAGA ACGGAGTCAT CACCGTAACA GATATGACAG GGAAAAATTC CCGAGTTCTT 
CTAAGTTCCT TAAAACATCC GTCAAATATA GCAGTGGATC CAATAGAGAG GTTGATGTTT 
TGGTCTTCAG AGGTGACCGG CAGCCTTCAC AGAGCACACC TCAAAGGTGT TGATGTAAAA 
ACACTGCTGG AGACAGGGGG AATATCGGTG CTGACTCTGG ATGTCCTGGA CAAACGGCTC 
TTCTGGGTTC AGGACAGTGG CGAAGGAAGC CACGCTTACA TTCATTCCTG TGATTATGAG 
GGTGGCTCCG TCCGTCTTAT CAGGCATCAA GCACGGCACA GTTTGTCTTC AATGGCCTTT 
TTTGGTGATC GGATCTTCTA CTCAGTGTTG AAAAGCAAGG CGATTTGGAT AGCCAACAAA 
CACACGGGGA AGGACACGGT CAGGATTAAC CTCCATCCAT CCTTTGTGAC ACCTGGAAAA 
CTGATGGTAG TACACCCTCG TGCACAGCCC AGGACAGAGG ACGCTGCTAA GGATCCTGAC 
CCCGAACTTC TCAAACAGAG GGGAAGACCA TGCCGCTTCG GTCTCTGTGA GCGAGACCCC 
AAGTCCCACT CGAGCGCATG CGCTGAGGGC TACACGTTAA GCCGAGACCG GAAGTACTGC 
GAAGATGTCA ATGAATGTGC CACTCAGAAT CACGGCTGTA CTCTTGGGTG TGAAAACACC 
CCTGGATCCT ATCACTGCAC ATGCCCCACA GGATTTGTTC TGCTTCCTGA TGGGAAACAA 
TGTCACGAAC TTGTTTCCTG CCCAGGCAAC GTATCAAAGT GCAGTCATGG CTGTGTCCTG 
ACATCAGATG GTCCCCGGTG CATCTGTCCT GCAGGTTCAG TGCTTGGGAG AGATGGGAAG 
ACTTGCACTG GTTGTTCATC GCCTGACAAT GGTGGATGCA GCCAGATCTG TCTTCCTCTC 
AGGCCAGGAT CCTGGGAATG TGATTGCTTT CCTGGGTATG ACCTACAGTC AGACCGAAAG 
AGCTGTGCAG CTTCAGGACC ACAGCCACTT TTACTGTTTG CAAATTCCCA GGACATCCGA 
CACATGCATT TTGATGGAAC AGACTACAAA GTTCTGCTCA GCCGGCAGAT GGGAATGGTT 
TTTGCCTTGG ATTATGACCC TGTGGAAAGC AAGATATATT TTGCACAGAC AGCCCTGAAG 
TGGATAGAGA GGGCTAATAT GGATGGGTCC CAGCGAGAAA GACTGATCAC AGAAGGAGTA 
GATACGCTTG AAGGTCTTGC CCTGGACTGG ATTGGCCGGA GAATCTACTG GACAGACAGT 
GGGAAGTCTG TTGTTGGAGG GAGCGATCTG AGCGGGAAGC ATCATCGAAT AATCATCCAG 
GAGAGAATCT CGAGGCCGCG AGGAATAGCT GTGCATCCAA GGGCCAGGAG ACTGTTCTGG 
ACGGACGTAG GGATGTCTCC ACGGATTGAA AGCGCTTCCC TTCAAGGTTC CGACCGGGTG 
CTGATAGCCA GCTCCAATCT ACTGGAACCC AGTGGAATCA CGATTGACTA CTTAACAGAC 
ACTTTGTACT GGTGTGACAC CAAGAGGTCT GTGATTGAAA TGGCCAATCT GGATGGCTCC 
AAACGCCGAA GACTTATCCA GAACGACGTA GGTCACCCCT TCTCTCTAGC CGTGTTTGAG 
GATCACCTGT GGGTCTCGGA TTGGGCTATC CCATCGGTAA TAAGGGTGAA CAAGAGGACT 
GGCCAAAACA GGGTACGTCT TCAAGGCAGC ATGCTGAAGC CCTCGTCACT GGTTGTGGTC 
CATCCATTGG CAAAACCAGG TGCAGATCCC TGCTTATACA GGAATGGAGG CTGTGAACAC 
ATCTGCCAAG AGAGCCTGGG CACAGCTCGG TGTTTGTGTC GTGAAGGTTT TGTGAAGGCC 
TGGGATGGGA AAATGTGTCT CCCTCAGGAT TATCCAATCC TGTCAGGTGA AAATGCTGAT 
CTTAGTAAAG AGGTGACATC ACTGAGCAAC TCCACTCAGG CTGAAGTACC AGACGATGAT 
GGGACAGAAT CTTCCACACT AGTGGCTGAA ATCATGGTGT CAGGCATGAA CTATGAAGAT 
GACTGTGGTC CCGGGGGGTG TGGAAGCCAT GCTCGATGCG TTTCAGACGG AGAGACTGCT 
GAGTGTCAGT GTCTGAAAGG GTTTGCCAGG GATGGAAACC TGTGTTCTGA TATAGATGAG 
TGTGTGCTGG CTAGATCGGA CTGCCCCAGC ACCTCGTCCA GGTGCATCAA CACTGAAGGT 
GGCTACGTCT GCAGATGCTC AGAAGGCTAC GAAGGAGACG GGATCTCCTG TTTCGATATT 
GACGAGTGCC AGCGGGGGGC GCACAACTGC GGTGAGAATG CCGCCTGCAC CAACACGGAG 
GGAGGCTACA ACTGCACCTG CGCAGGCCGC CCATCCTCGC CCGGACTGAG TTGCCCTGAC 
TCTACCGCAC CCTCTCTCCT TGGGGAAGAT GGCCACCATT TGGACCGAAA TAGTTATCCA 
GGATGCCCAT CCTCATATGA TGGATACTGC CTCAATGGTG GCGTGTGCAT GCATATTGAA 
TCACTGGACA GCTACACATG CAACTGTGTT ATTGGCTATT CTGGGGATCG ATGCCAGACT 
CGAGACCTAC GATGGTGGGA ACTGCGTCAT GCTGGCTACG GGCAGAAGCA TGACATCATG 
GTGGTGGCTG TCTGCATGGT GGCACTGGTC CTGCTGCTCG TCTTGGGGAT GTGGGGGACT 
TACTACTACA GGACTCGGAA GCAGCTATCA AACCCCCCAA AGAACCCTTG TGATGAGCCA 
AGCGGAAGTG TGAGCAGCAG CGGACCCAAC AGCAGCAGCG GGGCAGCTGT GGCTTCTTGT 
CCCCAACCTT GGTTTGTGGT CCTAGAGAAA CACCAAGACC CCAAGAATGG GAGTCTGCCT 
GCGGATGGTA CGAATGGTGC AGTAGTAGAT GCTGGCCTGT CTCCCTCCCT GCAGCTCGGG 
TCAGTGCATC TGACTTCATG GAGACAGAAG CCCCACATAG ATGGAATGGG CACAGGGCAA 
AGCTGCTGGA TTCCACCATC AAGTGACAGA GGACCCCAGG AAATAGAGGG AAACTCCCAC 
CTACCCTCCT ACAGACCTGT GGGGCCGGAG AAGCTGCATT CTCTCCAGTC AGCTAATGGA 
TCGTGTCACG AAAGGGCTCC AGACCTGCCA CGGCAGACAG AGCCAGTTCA GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Egf ( XM_006500990.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006501053.1 Mus musculus epidermal growth factor (Egf), transcript variant X4, mRNA.

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CloneID	OMu31618
Clone ID Related Accession (Same CDS sequence)	XM_006500990.3 , XM_006500990.4
Accession Version	XM_006500990.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3531bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	pro-epidermal growth factor isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000069.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006500990.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCCTGGG GCCGAAGGCC AACCTGGCTG TTGCTCGCCT TCCTGCTGGT GTTTTTAAAG 
ATTAGCATAC TCAGCGTCAC AGCATGGCAG ACCGGGAACT GTCAGCCAGG TCCTCTCGAG 
AGAAGCGAGA GAAGCGGGAC TTGTGCCGGT CCTGCCCCCT TCCTAGTTTT CTCACAAGGA 
AAGAGCATCT CTCGGATTGA CCCAGATGGA ACAAATCACC AGCAATTGGT GGTGGATGCT 
GGCATCTCAG CAGACATGGA TATTCATTAT AAAAAAGAGA GACTCTATTG GGTGGATGTA 
GAAAGACAAG TTTTGCTAAG AGTTTTCCTT AACGGGACAG GACTAGAGAA AGTGTGCAAT 
GTAGAGAGGA AGGTGTCTGG GCTGGCCATA GACTGGATAG ATGATGAAGT TCTCTGGGTA 
GACCAACAGA ACGGAGTCAT CACCGTAACA GATATGACAG GGAAAAATTC CCGAGTTCTT 
CTAAGTTCCT TAAAACATCC GTCAAATATA GCAGTGGATC CAATAGAGAG GTTGATGTTT 
TGGTCTTCAG AGGTGACCGG CAGCCTTCAC AGAGCACACC TCAAAGGTGT TGATGTAAAA 
ACACTGCTGG AGACAGGGGG AATATCGGTG CTGACTCTGG ATGTCCTGGA CAAACGGCTC 
TTCTGGGTTC AGGACAGTGG CGAAGGAAGC CACGCTTACA TTCATTCCTG TGATTATGAG 
GGTGGCTCCG TCCGTCTTAT CAGGCATCAA GCACGGCACA GTTTGTCTTC AATGGCCTTT 
TTTGGTGATC GGATCTTCTA CTCAGTGTTG AAAAGCAAGG CGATTTGGAT AGCCAACAAA 
CACACGGGGA AGGACACGGT CAGGATTAAC CTCCATCCAT CCTTTGTGAC ACCTGGAAAA 
CTGATGGTAG TACACCCTCG TGCACAGCCC AGGACAGAGG ACGCTGCTAA GGATCCTGAC 
CCCGAACTTC TCAAACAGAG GGGAAGACCA TGCCGCTTCG GTCTCTGTGA GCGAGACCCC 
AAGTCCCACT CGAGCGCATG CGCTGAGGGC TACACGTTAA GCCGAGACCG GAAGTACTGC 
GAAGATGTCA ATGAATGTGC CACTCAGAAT CACGGCTGTA CTCTTGGGTG TGAAAACACC 
CCTGGATCCT ATCACTGCAC ATGCCCCACA GGATTTGTTC TGCTTCCTGA TGGGAAACAA 
TGTCACGAAC TTGTTTCCTG CCCAGGCAAC GTATCAAAGT GCAGTCATGG CTGTGTCCTG 
ACATCAGATG GTCCCCGGTG CATCTGTCCT GCAGGTTCAG TGCTTGGGAG AGATGGGAAG 
ACTTGCACTG GTTGTTCATC GCCTGACAAT GGTGGATGCA GCCAGATCTG TCTTCCTCTC 
AGGCCAGGAT CCTGGGAATG TGATTGCTTT CCTGGGTATG ACCTACAGTC AGACCGAAAG 
AGCTGTGCAG CTTCAGGACC ACAGCCACTT TTACTGTTTG CAAATTCCCA GGACATCCGA 
CACATGCATT TTGATGGAAC AGACTACAAA GTTCTGCTCA GCCGGCAGAT GGGAATGGTT 
TTTGCCTTGG ATTATGACCC TGTGGAAAGC AAGATATATT TTGCACAGAC AGCCCTGAAG 
TGGATAGAGA GGGCTAATAT GGATGGGTCC CAGCGAGAAA GACTGATCAC AGAAGGAGTA 
GATACGCTTG AAGGTCTTGC CCTGGACTGG ATTGGCCGGA GAATCTACTG GACAGACAGT 
GGGAAGTCTG TTGTTGGAGG GAGCGATCTG AGCGGGAAGC ATCATCGAAT AATCATCCAG 
GAGAGAATCT CGAGGCCGCG AGGAATAGCT GTGCATCCAA GGGCCAGGAG ACTGTTCTGG 
ACGGACGTAG GGATGTCTCC ACGGATTGAA AGCGCTTCCC TTCAAGGTTC CGACCGGGTG 
CTGATAGCCA GCTCCAATCT ACTGGAACCC AGTGGAATCA CGATTGACTA CTTAACAGAC 
ACTTTGTACT GGTGTGACAC CAAGAGGTCT GTGATTGAAA TGGCCAATCT GGATGGCTCC 
AAACGCCGAA GACTTATCCA GAACGACGTA GGTCACCCCT TCTCTCTAGC CGTGTTTGAG 
GATCACCTGT GGGTCTCGGA TTGGGCTATC CCATCGGTAA TAAGGGTGAA CAAGAGGACT 
GGCCAAAACA GGGTACGTCT TCAAGGCAGC ATGCTGAAGC CCTCGTCACT GGTTGTGGTC 
CATCCATTGG CAAAACCAGG TGCAGATCCC TGCTTATACA GGAATGGAGG CTGTGAACAC 
ATCTGCCAAG AGAGCCTGGG CACAGCTCGG TGTTTGTGTC GTGAAGGTTT TGTGAAGGCC 
TGGGATGGGA AAATGTGTCT CCCTCAGGAT TATCCAATCC TGTCAGGTGA AAATGCTGAT 
CTTAGTAAAG AGGTGACATC ACTGAGCAAC TCCACTCAGG CTGAAGTACC AGACGATGAT 
GGGACAGAAT CTTCCACACT AGTGGCTGAA ATCATGGTGT CAGGCATGAA CTATGAAGAT 
GACTGTGGTC CCGGGGGGTG TGGAAGCCAT GCTCGATGCG TTTCAGACGG AGAGACTGCT 
GAGTGTCAGT GTCTGAAAGG GTTTGCCAGG GATGGAAACC TGTGTTCTGA TATAGATGAG 
TGTGTGCTGG CTAGATCGGA CTGCCCCAGC ACCTCGTCCA GGTGCATCAA CACTGAAGGT 
GGCTACGTCT GCAGATGCTC AGAAGGCTAC GAAGGAGACG GGATCTCCTG TTTCGACTCT 
ACCGCACCCT CTCTCCTTGG GGAAGATGGC CACCATTTGG ACCGAAATAG TTATCCAGGA 
TGCCCATCCT CATATGATGG ATACTGCCTC AATGGTGGCG TGTGCATGCA TATTGAATCA 
CTGGACAGCT ACACATGCAA CTGTGTTATT GGCTATTCTG GGGATCGATG CCAGACTCGA 
GACCTACGAT GGTGGGAACT GCGTCATGCT GGCTACGGGC AGAAGCATGA CATCATGGTG 
GTGGCTGTCT GCATGGTGGC ACTGGTCCTG CTGCTCGTCT TGGGGATGTG GGGGACTTAC 
TACTACAGGA CTCGGAAGCA GCTATCAAAC CCCCCAAAGA ACCCTTGTGA TGAGCCAAGC 
GGAAGTGTGA GCAGCAGCGG ACCCAACAGC AGCAGCGGGG CAGCTGTGGC TTCTTGTCCC 
CAACCTTGGT TTGTGGTCCT AGAGAAACAC CAAGACCCCA AGAATGGGAG TCTGCCTGCG 
GATGGTACGA ATGGTGCAGT AGTAGATGCT GGCCTGTCTC CCTCCCTGCA GCTCGGGTCA 
GTGCATCTGA CTTCATGGAG ACAGAAGCCC CACATAGATG GAATGGGCAC AGGGCAAAGC 
TGCTGGATTC CACCATCAAG TGACAGAGGA CCCCAGGAAA TAGAGGGAAA CTCCCACCTA 
CCCTCCTACA GACCTGTGGG GCCGGAGAAG CTGCATTCTC TCCAGTCAGC TAATGGATCG 
TGTCACGAAA GGGCTCCAGA CCTGCCACGG CAGACAGAGC CAGTTCAGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006501053.1
CDS	476..4006
Translation	MPWGRRPTWLLLAFLLVFLKISILSVTAWQTGNCQPGPLERSERSGTCAGPAPFLVFSQGKSISRIDPDGTNHQQLVVDAGISADMDIHYKKERLYWVDVERQVLLRVFLNGTGLEKVCNVERKVSGLAIDWIDDEVLWVDQQNGVITVTDMTGKNSRVLLSSLKHPSNIAVDPIERLMFWSSEVTGSLHRAHLKGVDVKTLLETGGISVLTLDVLDKRLFWVQDSGEGSHAYIHSCDYEGGSVRLIRHQARHSLSSMAFFGDRIFYSVLKSKAIWIANKHTGKDTVRINLHPSFVTPGKLMVVHPRAQPRTEDAAKDPDPELLKQRGRPCRFGLCERDPKSHSSACAEGYTLSRDRKYCEDVNECATQNHGCTLGCENTPGSYHCTCPTGFVLLPDGKQCHELVSCPGNVSKCSHGCVLTSDGPRCICPAGSVLGRDGKTCTGCSSPDNGGCSQICLPLRPGSWECDCFPGYDLQSDRKSCAASGPQPLLLFANSQDIRHMHFDGTDYKVLLSRQMGMVFALDYDPVESKIYFAQTALKWIERANMDGSQRERLITEGVDTLEGLALDWIGRRIYWTDSGKSVVGGSDLSGKHHRIIIQERISRPRGIAVHPRARRLFWTDVGMSPRIESASLQGSDRVLIASSNLLEPSGITIDYLTDTLYWCDTKRSVIEMANLDGSKRRRLIQNDVGHPFSLAVFEDHLWVSDWAIPSVIRVNKRTGQNRVRLQGSMLKPSSLVVVHPLAKPGADPCLYRNGGCEHICQESLGTARCLCREGFVKAWDGKMCLPQDYPILSGENADLSKEVTSLSNSTQAEVPDDDGTESSTLVAEIMVSGMNYEDDCGPGGCGSHARCVSDGETAECQCLKGFARDGNLCSDIDECVLARSDCPSTSSRCINTEGGYVCRCSEGYEGDGISCFDSTAPSLLGEDGHHLDRNSYPGCPSSYDGYCLNGGVCMHIESLDSYTCNCVIGYSGDRCQTRDLRWWELRHAGYGQKHDIMVVAVCMVALVLLLVLGMWGTYYYRTRKQLSNPPKNPCDEPSGSVSSSGPNSSSGAAVASCPQPWFVVLEKHQDPKNGSLPADGTNGAVVDAGLSPSLQLGSVHLTSWRQKPHIDGMGTGQSCWIPPSSDRGPQEIEGNSHLPSYRPVGPEKLHSLQSANGSCHERAPDLPRQTEPVQ

Target ORF information:

RefSeq Version	XM_006500990.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus epidermal growth factor (Egf), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006500990.3

ORF Insert Sequence:

ATGCCCTGGG GCCGAAGGCC AACCTGGCTG TTGCTCGCCT TCCTGCTGGT GTTTTTAAAG 
ATTAGCATAC TCAGCGTCAC AGCATGGCAG ACCGGGAACT GTCAGCCAGG TCCTCTCGAG 
AGAAGCGAGA GAAGCGGGAC TTGTGCCGGT CCTGCCCCCT TCCTAGTTTT CTCACAAGGA 
AAGAGCATCT CTCGGATTGA CCCAGATGGA ACAAATCACC AGCAATTGGT GGTGGATGCT 
GGCATCTCAG CAGACATGGA TATTCATTAT AAAAAAGAGA GACTCTATTG GGTGGATGTA 
GAAAGACAAG TTTTGCTAAG AGTTTTCCTT AACGGGACAG GACTAGAGAA AGTGTGCAAT 
GTAGAGAGGA AGGTGTCTGG GCTGGCCATA GACTGGATAG ATGATGAAGT TCTCTGGGTA 
GACCAACAGA ACGGAGTCAT CACCGTAACA GATATGACAG GGAAAAATTC CCGAGTTCTT 
CTAAGTTCCT TAAAACATCC GTCAAATATA GCAGTGGATC CAATAGAGAG GTTGATGTTT 
TGGTCTTCAG AGGTGACCGG CAGCCTTCAC AGAGCACACC TCAAAGGTGT TGATGTAAAA 
ACACTGCTGG AGACAGGGGG AATATCGGTG CTGACTCTGG ATGTCCTGGA CAAACGGCTC 
TTCTGGGTTC AGGACAGTGG CGAAGGAAGC CACGCTTACA TTCATTCCTG TGATTATGAG 
GGTGGCTCCG TCCGTCTTAT CAGGCATCAA GCACGGCACA GTTTGTCTTC AATGGCCTTT 
TTTGGTGATC GGATCTTCTA CTCAGTGTTG AAAAGCAAGG CGATTTGGAT AGCCAACAAA 
CACACGGGGA AGGACACGGT CAGGATTAAC CTCCATCCAT CCTTTGTGAC ACCTGGAAAA 
CTGATGGTAG TACACCCTCG TGCACAGCCC AGGACAGAGG ACGCTGCTAA GGATCCTGAC 
CCCGAACTTC TCAAACAGAG GGGAAGACCA TGCCGCTTCG GTCTCTGTGA GCGAGACCCC 
AAGTCCCACT CGAGCGCATG CGCTGAGGGC TACACGTTAA GCCGAGACCG GAAGTACTGC 
GAAGATGTCA ATGAATGTGC CACTCAGAAT CACGGCTGTA CTCTTGGGTG TGAAAACACC 
CCTGGATCCT ATCACTGCAC ATGCCCCACA GGATTTGTTC TGCTTCCTGA TGGGAAACAA 
TGTCACGAAC TTGTTTCCTG CCCAGGCAAC GTATCAAAGT GCAGTCATGG CTGTGTCCTG 
ACATCAGATG GTCCCCGGTG CATCTGTCCT GCAGGTTCAG TGCTTGGGAG AGATGGGAAG 
ACTTGCACTG GTTGTTCATC GCCTGACAAT GGTGGATGCA GCCAGATCTG TCTTCCTCTC 
AGGCCAGGAT CCTGGGAATG TGATTGCTTT CCTGGGTATG ACCTACAGTC AGACCGAAAG 
AGCTGTGCAG CTTCAGGACC ACAGCCACTT TTACTGTTTG CAAATTCCCA GGACATCCGA 
CACATGCATT TTGATGGAAC AGACTACAAA GTTCTGCTCA GCCGGCAGAT GGGAATGGTT 
TTTGCCTTGG ATTATGACCC TGTGGAAAGC AAGATATATT TTGCACAGAC AGCCCTGAAG 
TGGATAGAGA GGGCTAATAT GGATGGGTCC CAGCGAGAAA GACTGATCAC AGAAGGAGTA 
GATACGCTTG AAGGTCTTGC CCTGGACTGG ATTGGCCGGA GAATCTACTG GACAGACAGT 
GGGAAGTCTG TTGTTGGAGG GAGCGATCTG AGCGGGAAGC ATCATCGAAT AATCATCCAG 
GAGAGAATCT CGAGGCCGCG AGGAATAGCT GTGCATCCAA GGGCCAGGAG ACTGTTCTGG 
ACGGACGTAG GGATGTCTCC ACGGATTGAA AGCGCTTCCC TTCAAGGTTC CGACCGGGTG 
CTGATAGCCA GCTCCAATCT ACTGGAACCC AGTGGAATCA CGATTGACTA CTTAACAGAC 
ACTTTGTACT GGTGTGACAC CAAGAGGTCT GTGATTGAAA TGGCCAATCT GGATGGCTCC 
AAACGCCGAA GACTTATCCA GAACGACGTA GGTCACCCCT TCTCTCTAGC CGTGTTTGAG 
GATCACCTGT GGGTCTCGGA TTGGGCTATC CCATCGGTAA TAAGGGTGAA CAAGAGGACT 
GGCCAAAACA GGGTACGTCT TCAAGGCAGC ATGCTGAAGC CCTCGTCACT GGTTGTGGTC 
CATCCATTGG CAAAACCAGG TGCAGATCCC TGCTTATACA GGAATGGAGG CTGTGAACAC 
ATCTGCCAAG AGAGCCTGGG CACAGCTCGG TGTTTGTGTC GTGAAGGTTT TGTGAAGGCC 
TGGGATGGGA AAATGTGTCT CCCTCAGGAT TATCCAATCC TGTCAGGTGA AAATGCTGAT 
CTTAGTAAAG AGGTGACATC ACTGAGCAAC TCCACTCAGG CTGAAGTACC AGACGATGAT 
GGGACAGAAT CTTCCACACT AGTGGCTGAA ATCATGGTGT CAGGCATGAA CTATGAAGAT 
GACTGTGGTC CCGGGGGGTG TGGAAGCCAT GCTCGATGCG TTTCAGACGG AGAGACTGCT 
GAGTGTCAGT GTCTGAAAGG GTTTGCCAGG GATGGAAACC TGTGTTCTGA TATAGATGAG 
TGTGTGCTGG CTAGATCGGA CTGCCCCAGC ACCTCGTCCA GGTGCATCAA CACTGAAGGT 
GGCTACGTCT GCAGATGCTC AGAAGGCTAC GAAGGAGACG GGATCTCCTG TTTCGACTCT 
ACCGCACCCT CTCTCCTTGG GGAAGATGGC CACCATTTGG ACCGAAATAG TTATCCAGGA 
TGCCCATCCT CATATGATGG ATACTGCCTC AATGGTGGCG TGTGCATGCA TATTGAATCA 
CTGGACAGCT ACACATGCAA CTGTGTTATT GGCTATTCTG GGGATCGATG CCAGACTCGA 
GACCTACGAT GGTGGGAACT GCGTCATGCT GGCTACGGGC AGAAGCATGA CATCATGGTG 
GTGGCTGTCT GCATGGTGGC ACTGGTCCTG CTGCTCGTCT TGGGGATGTG GGGGACTTAC 
TACTACAGGA CTCGGAAGCA GCTATCAAAC CCCCCAAAGA ACCCTTGTGA TGAGCCAAGC 
GGAAGTGTGA GCAGCAGCGG ACCCAACAGC AGCAGCGGGG CAGCTGTGGC TTCTTGTCCC 
CAACCTTGGT TTGTGGTCCT AGAGAAACAC CAAGACCCCA AGAATGGGAG TCTGCCTGCG 
GATGGTACGA ATGGTGCAGT AGTAGATGCT GGCCTGTCTC CCTCCCTGCA GCTCGGGTCA 
GTGCATCTGA CTTCATGGAG ACAGAAGCCC CACATAGATG GAATGGGCAC AGGGCAAAGC 
TGCTGGATTC CACCATCAAG TGACAGAGGA CCCCAGGAAA TAGAGGGAAA CTCCCACCTA 
CCCTCCTACA GACCTGTGGG GCCGGAGAAG CTGCATTCTC TCCAGTCAGC TAATGGATCG 
TGTCACGAAA GGGCTCCAGA CCTGCCACGG CAGACAGAGC CAGTTCAGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Egf ( XM_011240010.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011238312.1 Mus musculus epidermal growth factor (Egf), transcript variant X1, mRNA.

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CloneID	OMu67604
Clone ID Related Accession (Same CDS sequence)	XM_011240010.2 , XM_011240010.3
Accession Version	XM_011240010.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3666bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	pro-epidermal growth factor isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000069.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_011240010.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCCTGGG GCCGAAGGCC AACCTGGCTG TTGCTCGCCT TCCTGCTGGT GTTTTTAAAG 
ATTAGCATAC TCAGCGTCAC AGCATGGCAG ACCGGGAACT GTCAGCCAGG TCCTCTCGAG 
AGAAGCGAGA GAAGCGGGAC TTGTGCCGGT CCTGCCCCCT TCCTAGTTTT CTCACAAGGA 
AAGAGCATCT CTCGGATTGA CCCAGATGGA ACAAATCACC AGCAATTGGT GGTGGATGCT 
GGCATCTCAG CAGACATGGA TATTCATTAT AAAAAAGAGA GACTCTATTG GGTGGATGTA 
GAAAGACAAG TTTTGCTAAG AGTTTTCCTT AACGGGACAG GACTAGAGAA AGTGTGCAAT 
GTAGAGAGGA AGGTGTCTGG GCTGGCCATA GACTGGATAG ATGATGAAGT TCTCTGGGTA 
GACCAACAGA ACGGAGTCAT CACCGTAACA GATATGACAG GGAAAAATTC CCGAGTTCTT 
CTAAGTTCCT TAAAACATCC GTCAAATATA GCAGTGGATC CAATAGAGAG GTTGATGTTT 
TGGTCTTCAG AGGTGACCGG CAGCCTTCAC AGAGCACACC TCAAAGGTGT TGATGTAAAA 
ACACTGCTGG AGACAGGGGG AATATCGGTG CTGACTCTGG ATGTCCTGGA CAAACGGCTC 
TTCTGGGTTC AGGACAGTGG CGAAGGAAGC CACGCTTACA TTCATTCCTG TGATTATGAG 
GGTGGCTCCG TCCGTCTTAT CAGGCATCAA GCACGGCACA GTTTGTCTTC AATGGCCTTT 
TTTGGTGATC GGATCTTCTA CTCAGTGTTG AAAAGCAAGG CGATTTGGAT AGCCAACAAA 
CACACGGGGA AGGACACGGT CAGGATTAAC CTCCATCCAT CCTTTGTGAC ACCTGGAAAA 
CTGATGGTAG TACACCCTCG TGCACAGCCC AGGACAGAGG ACGCTGCTAA GGATCCTGGT 
GAGTCACTAG ACCCCGAACT TCTCAAACAG AGGGGAAGAC CATGCCGCTT CGGTCTCTGT 
GAGCGAGACC CCAAGTCCCA CTCGAGCGCA TGCGCTGAGG GCTACACGTT AAGCCGAGAC 
CGGAAGTACT GCGAAGATGT CAATGAATGT GCCACTCAGA ATCACGGCTG TACTCTTGGG 
TGTGAAAACA CCCCTGGATC CTATCACTGC ACATGCCCCA CAGGATTTGT TCTGCTTCCT 
GATGGGAAAC AATGTCACGA ACTTGTTTCC TGCCCAGGCA ACGTATCAAA GTGCAGTCAT 
GGCTGTGTCC TGACATCAGA TGGTCCCCGG TGCATCTGTC CTGCAGGTTC AGTGCTTGGG 
AGAGATGGGA AGACTTGCAC TGGTTGTTCA TCGCCTGACA ATGGTGGATG CAGCCAGATC 
TGTCTTCCTC TCAGGCCAGG ATCCTGGGAA TGTGATTGCT TTCCTGGGTA TGACCTACAG 
TCAGACCGAA AGAGCTGTGC AGCTTCAGGA CCACAGCCAC TTTTACTGTT TGCAAATTCC 
CAGGACATCC GACACATGCA TTTTGATGGA ACAGACTACA AAGTTCTGCT CAGCCGGCAG 
ATGGGAATGG TTTTTGCCTT GGATTATGAC CCTGTGGAAA GCAAGATATA TTTTGCACAG 
ACAGCCCTGA AGTGGATAGA GAGGGCTAAT ATGGATGGGT CCCAGCGAGA AAGACTGATC 
ACAGAAGGAG TAGATACGCT TGAAGGTCTT GCCCTGGACT GGATTGGCCG GAGAATCTAC 
TGGACAGACA GTGGGAAGTC TGTTGTTGGA GGGAGCGATC TGAGCGGGAA GCATCATCGA 
ATAATCATCC AGGAGAGAAT CTCGAGGCCG CGAGGAATAG CTGTGCATCC AAGGGCCAGG 
AGACTGTTCT GGACGGACGT AGGGATGTCT CCACGGATTG AAAGCGCTTC CCTTCAAGGT 
TCCGACCGGG TGCTGATAGC CAGCTCCAAT CTACTGGAAC CCAGTGGAAT CACGATTGAC 
TACTTAACAG ACACTTTGTA CTGGTGTGAC ACCAAGAGGT CTGTGATTGA AATGGCCAAT 
CTGGATGGCT CCAAACGCCG AAGACTTATC CAGAACGACG TAGGTCACCC CTTCTCTCTA 
GCCGTGTTTG AGGATCACCT GTGGGTCTCG GATTGGGCTA TCCCATCGGT AATAAGGGTG 
AACAAGAGGA CTGGCCAAAA CAGGGTACGT CTTCAAGGCA GCATGCTGAA GCCCTCGTCA 
CTGGTTGTGG TCCATCCATT GGCAAAACCA GGTGCAGATC CCTGCTTATA CAGGAATGGA 
GGCTGTGAAC ACATCTGCCA AGAGAGCCTG GGCACAGCTC GGTGTTTGTG TCGTGAAGGT 
TTTGTGAAGG CCTGGGATGG GAAAATGTGT CTCCCTCAGG ATTATCCAAT CCTGTCAGGT 
GAAAATGCTG ATCTTAGTAA AGAGGTGACA TCACTGAGCA ACTCCACTCA GGCTGAAGTA 
CCAGACGATG ATGGGACAGA ATCTTCCACA CTAGTGGCTG AAATCATGGT GTCAGGCATG 
AACTATGAAG ATGACTGTGG TCCCGGGGGG TGTGGAAGCC ATGCTCGATG CGTTTCAGAC 
GGAGAGACTG CTGAGTGTCA GTGTCTGAAA GGGTTTGCCA GGGATGGAAA CCTGTGTTCT 
GATATAGATG AGTGTGTGCT GGCTAGATCG GACTGCCCCA GCACCTCGTC CAGGTGCATC 
AACACTGAAG GTGGCTACGT CTGCAGATGC TCAGAAGGCT ACGAAGGAGA CGGGATCTCC 
TGTTTCGATA TTGACGAGTG CCAGCGGGGG GCGCACAACT GCGGTGAGAA TGCCGCCTGC 
ACCAACACGG AGGGAGGCTA CAACTGCACC TGCGCAGGCC GCCCATCCTC GCCCGGACTG 
AGTTGCCCTG ACTCTACCGC ACCCTCTCTC CTTGGGGAAG ATGGCCACCA TTTGGACCGA 
AATAGTTATC CAGGATGCCC ATCCTCATAT GATGGATACT GCCTCAATGG TGGCGTGTGC 
ATGCATATTG AATCACTGGA CAGCTACACA TGCAACTGTG TTATTGGCTA TTCTGGGGAT 
CGATGCCAGA CTCGAGACCT ACGATGGTGG GAACTGCGTC ATGCTGGCTA CGGGCAGAAG 
CATGACATCA TGGTGGTGGC TGTCTGCATG GTGGCACTGG TCCTGCTGCT CGTCTTGGGG 
ATGTGGGGGA CTTACTACTA CAGGACTCGG AAGCAGCTAT CAAACCCCCC AAAGAACCCT 
TGTGATGAGC CAAGCGGAAG TGTGAGCAGC AGCGGACCCA ACAGCAGCAG CGGGGCAGCT 
GTGGCTTCTT GTCCCCAACC TTGGTTTGTG GTCCTAGAGA AACACCAAGA CCCCAAGAAT 
GGGAGTCTGC CTGCGGATGG TACGAATGGT GCAGTAGTAG ATGCTGGCCT GTCTCCCTCC 
CTGCAGCTCG GGTCAGTGCA TCTGACTTCA TGGAGACAGA AGCCCCACAT AGATGGAATG 
GGCACAGGGC AAAGCTGCTG GATTCCACCA TCAAGTGACA GAGGACCCCA GGAAATAGAG 
GGAAACTCCC ACCTACCCTC CTACAGACCT GTGGGGCCGG AGAAGCTGCA TTCTCTCCAG 
TCAGCTAATG GATCGTGTCA CGAAAGGGCT CCAGACCTGC CACGGCAGAC AGAGCCAGTT 
CAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011238312.1
CDS	476..4141
Translation	MPWGRRPTWLLLAFLLVFLKISILSVTAWQTGNCQPGPLERSERSGTCAGPAPFLVFSQGKSISRIDPDGTNHQQLVVDAGISADMDIHYKKERLYWVDVERQVLLRVFLNGTGLEKVCNVERKVSGLAIDWIDDEVLWVDQQNGVITVTDMTGKNSRVLLSSLKHPSNIAVDPIERLMFWSSEVTGSLHRAHLKGVDVKTLLETGGISVLTLDVLDKRLFWVQDSGEGSHAYIHSCDYEGGSVRLIRHQARHSLSSMAFFGDRIFYSVLKSKAIWIANKHTGKDTVRINLHPSFVTPGKLMVVHPRAQPRTEDAAKDPGESLDPELLKQRGRPCRFGLCERDPKSHSSACAEGYTLSRDRKYCEDVNECATQNHGCTLGCENTPGSYHCTCPTGFVLLPDGKQCHELVSCPGNVSKCSHGCVLTSDGPRCICPAGSVLGRDGKTCTGCSSPDNGGCSQICLPLRPGSWECDCFPGYDLQSDRKSCAASGPQPLLLFANSQDIRHMHFDGTDYKVLLSRQMGMVFALDYDPVESKIYFAQTALKWIERANMDGSQRERLITEGVDTLEGLALDWIGRRIYWTDSGKSVVGGSDLSGKHHRIIIQERISRPRGIAVHPRARRLFWTDVGMSPRIESASLQGSDRVLIASSNLLEPSGITIDYLTDTLYWCDTKRSVIEMANLDGSKRRRLIQNDVGHPFSLAVFEDHLWVSDWAIPSVIRVNKRTGQNRVRLQGSMLKPSSLVVVHPLAKPGADPCLYRNGGCEHICQESLGTARCLCREGFVKAWDGKMCLPQDYPILSGENADLSKEVTSLSNSTQAEVPDDDGTESSTLVAEIMVSGMNYEDDCGPGGCGSHARCVSDGETAECQCLKGFARDGNLCSDIDECVLARSDCPSTSSRCINTEGGYVCRCSEGYEGDGISCFDIDECQRGAHNCGENAACTNTEGGYNCTCAGRPSSPGLSCPDSTAPSLLGEDGHHLDRNSYPGCPSSYDGYCLNGGVCMHIESLDSYTCNCVIGYSGDRCQTRDLRWWELRHAGYGQKHDIMVVAVCMVALVLLLVLGMWGTYYYRTRKQLSNPPKNPCDEPSGSVSSSGPNSSSGAAVASCPQPWFVVLEKHQDPKNGSLPADGTNGAVVDAGLSPSLQLGSVHLTSWRQKPHIDGMGTGQSCWIPPSSDRGPQEIEGNSHLPSYRPVGPEKLHSLQSANGSCHERAPDLPRQTEPVQ

Target ORF information:

RefSeq Version	XM_011240010.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus epidermal growth factor (Egf), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011240010.2

ORF Insert Sequence:

ATGCCCTGGG GCCGAAGGCC AACCTGGCTG TTGCTCGCCT TCCTGCTGGT GTTTTTAAAG 
ATTAGCATAC TCAGCGTCAC AGCATGGCAG ACCGGGAACT GTCAGCCAGG TCCTCTCGAG 
AGAAGCGAGA GAAGCGGGAC TTGTGCCGGT CCTGCCCCCT TCCTAGTTTT CTCACAAGGA 
AAGAGCATCT CTCGGATTGA CCCAGATGGA ACAAATCACC AGCAATTGGT GGTGGATGCT 
GGCATCTCAG CAGACATGGA TATTCATTAT AAAAAAGAGA GACTCTATTG GGTGGATGTA 
GAAAGACAAG TTTTGCTAAG AGTTTTCCTT AACGGGACAG GACTAGAGAA AGTGTGCAAT 
GTAGAGAGGA AGGTGTCTGG GCTGGCCATA GACTGGATAG ATGATGAAGT TCTCTGGGTA 
GACCAACAGA ACGGAGTCAT CACCGTAACA GATATGACAG GGAAAAATTC CCGAGTTCTT 
CTAAGTTCCT TAAAACATCC GTCAAATATA GCAGTGGATC CAATAGAGAG GTTGATGTTT 
TGGTCTTCAG AGGTGACCGG CAGCCTTCAC AGAGCACACC TCAAAGGTGT TGATGTAAAA 
ACACTGCTGG AGACAGGGGG AATATCGGTG CTGACTCTGG ATGTCCTGGA CAAACGGCTC 
TTCTGGGTTC AGGACAGTGG CGAAGGAAGC CACGCTTACA TTCATTCCTG TGATTATGAG 
GGTGGCTCCG TCCGTCTTAT CAGGCATCAA GCACGGCACA GTTTGTCTTC AATGGCCTTT 
TTTGGTGATC GGATCTTCTA CTCAGTGTTG AAAAGCAAGG CGATTTGGAT AGCCAACAAA 
CACACGGGGA AGGACACGGT CAGGATTAAC CTCCATCCAT CCTTTGTGAC ACCTGGAAAA 
CTGATGGTAG TACACCCTCG TGCACAGCCC AGGACAGAGG ACGCTGCTAA GGATCCTGGT 
GAGTCACTAG ACCCCGAACT TCTCAAACAG AGGGGAAGAC CATGCCGCTT CGGTCTCTGT 
GAGCGAGACC CCAAGTCCCA CTCGAGCGCA TGCGCTGAGG GCTACACGTT AAGCCGAGAC 
CGGAAGTACT GCGAAGATGT CAATGAATGT GCCACTCAGA ATCACGGCTG TACTCTTGGG 
TGTGAAAACA CCCCTGGATC CTATCACTGC ACATGCCCCA CAGGATTTGT TCTGCTTCCT 
GATGGGAAAC AATGTCACGA ACTTGTTTCC TGCCCAGGCA ACGTATCAAA GTGCAGTCAT 
GGCTGTGTCC TGACATCAGA TGGTCCCCGG TGCATCTGTC CTGCAGGTTC AGTGCTTGGG 
AGAGATGGGA AGACTTGCAC TGGTTGTTCA TCGCCTGACA ATGGTGGATG CAGCCAGATC 
TGTCTTCCTC TCAGGCCAGG ATCCTGGGAA TGTGATTGCT TTCCTGGGTA TGACCTACAG 
TCAGACCGAA AGAGCTGTGC AGCTTCAGGA CCACAGCCAC TTTTACTGTT TGCAAATTCC 
CAGGACATCC GACACATGCA TTTTGATGGA ACAGACTACA AAGTTCTGCT CAGCCGGCAG 
ATGGGAATGG TTTTTGCCTT GGATTATGAC CCTGTGGAAA GCAAGATATA TTTTGCACAG 
ACAGCCCTGA AGTGGATAGA GAGGGCTAAT ATGGATGGGT CCCAGCGAGA AAGACTGATC 
ACAGAAGGAG TAGATACGCT TGAAGGTCTT GCCCTGGACT GGATTGGCCG GAGAATCTAC 
TGGACAGACA GTGGGAAGTC TGTTGTTGGA GGGAGCGATC TGAGCGGGAA GCATCATCGA 
ATAATCATCC AGGAGAGAAT CTCGAGGCCG CGAGGAATAG CTGTGCATCC AAGGGCCAGG 
AGACTGTTCT GGACGGACGT AGGGATGTCT CCACGGATTG AAAGCGCTTC CCTTCAAGGT 
TCCGACCGGG TGCTGATAGC CAGCTCCAAT CTACTGGAAC CCAGTGGAAT CACGATTGAC 
TACTTAACAG ACACTTTGTA CTGGTGTGAC ACCAAGAGGT CTGTGATTGA AATGGCCAAT 
CTGGATGGCT CCAAACGCCG AAGACTTATC CAGAACGACG TAGGTCACCC CTTCTCTCTA 
GCCGTGTTTG AGGATCACCT GTGGGTCTCG GATTGGGCTA TCCCATCGGT AATAAGGGTG 
AACAAGAGGA CTGGCCAAAA CAGGGTACGT CTTCAAGGCA GCATGCTGAA GCCCTCGTCA 
CTGGTTGTGG TCCATCCATT GGCAAAACCA GGTGCAGATC CCTGCTTATA CAGGAATGGA 
GGCTGTGAAC ACATCTGCCA AGAGAGCCTG GGCACAGCTC GGTGTTTGTG TCGTGAAGGT 
TTTGTGAAGG CCTGGGATGG GAAAATGTGT CTCCCTCAGG ATTATCCAAT CCTGTCAGGT 
GAAAATGCTG ATCTTAGTAA AGAGGTGACA TCACTGAGCA ACTCCACTCA GGCTGAAGTA 
CCAGACGATG ATGGGACAGA ATCTTCCACA CTAGTGGCTG AAATCATGGT GTCAGGCATG 
AACTATGAAG ATGACTGTGG TCCCGGGGGG TGTGGAAGCC ATGCTCGATG CGTTTCAGAC 
GGAGAGACTG CTGAGTGTCA GTGTCTGAAA GGGTTTGCCA GGGATGGAAA CCTGTGTTCT 
GATATAGATG AGTGTGTGCT GGCTAGATCG GACTGCCCCA GCACCTCGTC CAGGTGCATC 
AACACTGAAG GTGGCTACGT CTGCAGATGC TCAGAAGGCT ACGAAGGAGA CGGGATCTCC 
TGTTTCGATA TTGACGAGTG CCAGCGGGGG GCGCACAACT GCGGTGAGAA TGCCGCCTGC 
ACCAACACGG AGGGAGGCTA CAACTGCACC TGCGCAGGCC GCCCATCCTC GCCCGGACTG 
AGTTGCCCTG ACTCTACCGC ACCCTCTCTC CTTGGGGAAG ATGGCCACCA TTTGGACCGA 
AATAGTTATC CAGGATGCCC ATCCTCATAT GATGGATACT GCCTCAATGG TGGCGTGTGC 
ATGCATATTG AATCACTGGA CAGCTACACA TGCAACTGTG TTATTGGCTA TTCTGGGGAT 
CGATGCCAGA CTCGAGACCT ACGATGGTGG GAACTGCGTC ATGCTGGCTA CGGGCAGAAG 
CATGACATCA TGGTGGTGGC TGTCTGCATG GTGGCACTGG TCCTGCTGCT CGTCTTGGGG 
ATGTGGGGGA CTTACTACTA CAGGACTCGG AAGCAGCTAT CAAACCCCCC AAAGAACCCT 
TGTGATGAGC CAAGCGGAAG TGTGAGCAGC AGCGGACCCA ACAGCAGCAG CGGGGCAGCT 
GTGGCTTCTT GTCCCCAACC TTGGTTTGTG GTCCTAGAGA AACACCAAGA CCCCAAGAAT 
GGGAGTCTGC CTGCGGATGG TACGAATGGT GCAGTAGTAG ATGCTGGCCT GTCTCCCTCC 
CTGCAGCTCG GGTCAGTGCA TCTGACTTCA TGGAGACAGA AGCCCCACAT AGATGGAATG 
GGCACAGGGC AAAGCTGCTG GATTCCACCA TCAAGTGACA GAGGACCCCA GGAAATAGAG 
GGAAACTCCC ACCTACCCTC CTACAGACCT GTGGGGCCGG AGAAGCTGCA TTCTCTCCAG 
TCAGCTAATG GATCGTGTCA CGAAAGGGCT CCAGACCTGC CACGGCAGAC AGAGCCAGTT 
CAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Egf ( XM_011240011.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011238313.1 Mus musculus epidermal growth factor (Egf), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OMu67605
Clone ID Related Accession (Same CDS sequence)	XM_011240011.2 , XM_011240011.3
Accession Version	XM_011240011.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3543bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	pro-epidermal growth factor isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000069.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_011240011.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCCTGGG GCCGAAGGCC AACCTGGCTG TTGCTCGCCT TCCTGCTGGT GTTTTTAAAG 
ATTAGCATAC TCAGCGTCAC AGCATGGCAG ACCGGGAACT GTCAGCCAGG TCCTCTCGAG 
AGAAGCGAGA GAAGCGGGAC TTGTGCCGGT CCTGCCCCCT TCCTAGTTTT CTCACAAGGA 
AAGAGCATCT CTCGGATTGA CCCAGATGGA ACAAATCACC AGCAATTGGT GGTGGATGCT 
GGCATCTCAG CAGACATGGA TATTCATTAT AAAAAAGAGA GACTCTATTG GGTGGATGTA 
GAAAGACAAG TTTTGCTAAG AGTTTTCCTT AACGGGACAG GACTAGAGAA AGTGTGCAAT 
GTAGAGAGGA AGGTGTCTGG GCTGGCCATA GACTGGATAG ATGATGAAGT TCTCTGGGTA 
GACCAACAGA ACGGAGTCAT CACCGTAACA GATATGACAG GGAAAAATTC CCGAGTTCTT 
CTAAGTTCCT TAAAACATCC GTCAAATATA GCAGTGGATC CAATAGAGAG GTTGATGTTT 
TGGTCTTCAG AGGTGACCGG CAGCCTTCAC AGAGCACACC TCAAAGGTGT TGATGTAAAA 
ACACTGCTGG AGACAGGGGG AATATCGGTG CTGACTCTGG ATGTCCTGGA CAAACGGCTC 
TTCTGGGTTC AGGACAGTGG CGAAGGAAGC CACGCTTACA TTCATTCCTG TGATTATGAG 
GGTGGCTCCG TCCGTCTTAT CAGGCATCAA GCACGGCACA GTTTGTCTTC AATGGCCTTT 
TTTGGTGATC GGATCTTCTA CTCAGTGTTG AAAAGCAAGG CGATTTGGAT AGCCAACAAA 
CACACGGGGA AGGACACGGT CAGGATTAAC CTCCATCCAT CCTTTGTGAC ACCTGGAAAA 
CTGATGGTAG TACACCCTCG TGCACAGCCC AGGACAGAGG ACGCTGCTAA GGATCCTGGT 
GAGTCACTAG ACCCCGAACT TCTCAAACAG AGGGGAAGAC CATGCCGCTT CGGTCTCTGT 
GAGCGAGACC CCAAGTCCCA CTCGAGCGCA TGCGCTGAGG GCTACACGTT AAGCCGAGAC 
CGGAAGTACT GCGAAGATGT CAATGAATGT GCCACTCAGA ATCACGGCTG TACTCTTGGG 
TGTGAAAACA CCCCTGGATC CTATCACTGC ACATGCCCCA CAGGATTTGT TCTGCTTCCT 
GATGGGAAAC AATGTCACGA ACTTGTTTCC TGCCCAGGCA ACGTATCAAA GTGCAGTCAT 
GGCTGTGTCC TGACATCAGA TGGTCCCCGG TGCATCTGTC CTGCAGGTTC AGTGCTTGGG 
AGAGATGGGA AGACTTGCAC TGGTTGTTCA TCGCCTGACA ATGGTGGATG CAGCCAGATC 
TGTCTTCCTC TCAGGCCAGG ATCCTGGGAA TGTGATTGCT TTCCTGGGTA TGACCTACAG 
TCAGACCGAA AGAGCTGTGC AGCTTCAGGA CCACAGCCAC TTTTACTGTT TGCAAATTCC 
CAGGACATCC GACACATGCA TTTTGATGGA ACAGACTACA AAGTTCTGCT CAGCCGGCAG 
ATGGGAATGG TTTTTGCCTT GGATTATGAC CCTGTGGAAA GCAAGATATA TTTTGCACAG 
ACAGCCCTGA AGTGGATAGA GAGGGCTAAT ATGGATGGGT CCCAGCGAGA AAGACTGATC 
ACAGAAGGAG TAGATACGCT TGAAGGTCTT GCCCTGGACT GGATTGGCCG GAGAATCTAC 
TGGACAGACA GTGGGAAGTC TGTTGTTGGA GGGAGCGATC TGAGCGGGAA GCATCATCGA 
ATAATCATCC AGGAGAGAAT CTCGAGGCCG CGAGGAATAG CTGTGCATCC AAGGGCCAGG 
AGACTGTTCT GGACGGACGT AGGGATGTCT CCACGGATTG AAAGCGCTTC CCTTCAAGGT 
TCCGACCGGG TGCTGATAGC CAGCTCCAAT CTACTGGAAC CCAGTGGAAT CACGATTGAC 
TACTTAACAG ACACTTTGTA CTGGTGTGAC ACCAAGAGGT CTGTGATTGA AATGGCCAAT 
CTGGATGGCT CCAAACGCCG AAGACTTATC CAGAACGACG TAGGTCACCC CTTCTCTCTA 
GCCGTGTTTG AGGATCACCT GTGGGTCTCG GATTGGGCTA TCCCATCGGT AATAAGGGTG 
AACAAGAGGA CTGGCCAAAA CAGGGTACGT CTTCAAGGCA GCATGCTGAA GCCCTCGTCA 
CTGGTTGTGG TCCATCCATT GGCAAAACCA GGTGCAGATC CCTGCTTATA CAGGAATGGA 
GGCTGTGAAC ACATCTGCCA AGAGAGCCTG GGCACAGCTC GGTGTTTGTG TCGTGAAGGT 
TTTGTGAAGG CCTGGGATGG GAAAATGTGT CTCCCTCAGG ATTATCCAAT CCTGTCAGGT 
GAAAATGCTG ATCTTAGTAA AGAGGTGACA TCACTGAGCA ACTCCACTCA GGCTGAAGTA 
CCAGACGATG ATGGGACAGA ATCTTCCACA CTAGTGGCTG AAATCATGGT GTCAGGCATG 
AACTATGAAG ATGACTGTGG TCCCGGGGGG TGTGGAAGCC ATGCTCGATG CGTTTCAGAC 
GGAGAGACTG CTGAGTGTCA GTGTCTGAAA GGGTTTGCCA GGGATGGAAA CCTGTGTTCT 
GATATAGATG AGTGTGTGCT GGCTAGATCG GACTGCCCCA GCACCTCGTC CAGGTGCATC 
AACACTGAAG GTGGCTACGT CTGCAGATGC TCAGAAGGCT ACGAAGGAGA CGGGATCTCC 
TGTTTCGACT CTACCGCACC CTCTCTCCTT GGGGAAGATG GCCACCATTT GGACCGAAAT 
AGTTATCCAG GATGCCCATC CTCATATGAT GGATACTGCC TCAATGGTGG CGTGTGCATG 
CATATTGAAT CACTGGACAG CTACACATGC AACTGTGTTA TTGGCTATTC TGGGGATCGA 
TGCCAGACTC GAGACCTACG ATGGTGGGAA CTGCGTCATG CTGGCTACGG GCAGAAGCAT 
GACATCATGG TGGTGGCTGT CTGCATGGTG GCACTGGTCC TGCTGCTCGT CTTGGGGATG 
TGGGGGACTT ACTACTACAG GACTCGGAAG CAGCTATCAA ACCCCCCAAA GAACCCTTGT 
GATGAGCCAA GCGGAAGTGT GAGCAGCAGC GGACCCAACA GCAGCAGCGG GGCAGCTGTG 
GCTTCTTGTC CCCAACCTTG GTTTGTGGTC CTAGAGAAAC ACCAAGACCC CAAGAATGGG 
AGTCTGCCTG CGGATGGTAC GAATGGTGCA GTAGTAGATG CTGGCCTGTC TCCCTCCCTG 
CAGCTCGGGT CAGTGCATCT GACTTCATGG AGACAGAAGC CCCACATAGA TGGAATGGGC 
ACAGGGCAAA GCTGCTGGAT TCCACCATCA AGTGACAGAG GACCCCAGGA AATAGAGGGA 
AACTCCCACC TACCCTCCTA CAGACCTGTG GGGCCGGAGA AGCTGCATTC TCTCCAGTCA 
GCTAATGGAT CGTGTCACGA AAGGGCTCCA GACCTGCCAC GGCAGACAGA GCCAGTTCAG 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011238313.1
CDS	476..4018
Translation	MPWGRRPTWLLLAFLLVFLKISILSVTAWQTGNCQPGPLERSERSGTCAGPAPFLVFSQGKSISRIDPDGTNHQQLVVDAGISADMDIHYKKERLYWVDVERQVLLRVFLNGTGLEKVCNVERKVSGLAIDWIDDEVLWVDQQNGVITVTDMTGKNSRVLLSSLKHPSNIAVDPIERLMFWSSEVTGSLHRAHLKGVDVKTLLETGGISVLTLDVLDKRLFWVQDSGEGSHAYIHSCDYEGGSVRLIRHQARHSLSSMAFFGDRIFYSVLKSKAIWIANKHTGKDTVRINLHPSFVTPGKLMVVHPRAQPRTEDAAKDPGESLDPELLKQRGRPCRFGLCERDPKSHSSACAEGYTLSRDRKYCEDVNECATQNHGCTLGCENTPGSYHCTCPTGFVLLPDGKQCHELVSCPGNVSKCSHGCVLTSDGPRCICPAGSVLGRDGKTCTGCSSPDNGGCSQICLPLRPGSWECDCFPGYDLQSDRKSCAASGPQPLLLFANSQDIRHMHFDGTDYKVLLSRQMGMVFALDYDPVESKIYFAQTALKWIERANMDGSQRERLITEGVDTLEGLALDWIGRRIYWTDSGKSVVGGSDLSGKHHRIIIQERISRPRGIAVHPRARRLFWTDVGMSPRIESASLQGSDRVLIASSNLLEPSGITIDYLTDTLYWCDTKRSVIEMANLDGSKRRRLIQNDVGHPFSLAVFEDHLWVSDWAIPSVIRVNKRTGQNRVRLQGSMLKPSSLVVVHPLAKPGADPCLYRNGGCEHICQESLGTARCLCREGFVKAWDGKMCLPQDYPILSGENADLSKEVTSLSNSTQAEVPDDDGTESSTLVAEIMVSGMNYEDDCGPGGCGSHARCVSDGETAECQCLKGFARDGNLCSDIDECVLARSDCPSTSSRCINTEGGYVCRCSEGYEGDGISCFDSTAPSLLGEDGHHLDRNSYPGCPSSYDGYCLNGGVCMHIESLDSYTCNCVIGYSGDRCQTRDLRWWELRHAGYGQKHDIMVVAVCMVALVLLLVLGMWGTYYYRTRKQLSNPPKNPCDEPSGSVSSSGPNSSSGAAVASCPQPWFVVLEKHQDPKNGSLPADGTNGAVVDAGLSPSLQLGSVHLTSWRQKPHIDGMGTGQSCWIPPSSDRGPQEIEGNSHLPSYRPVGPEKLHSLQSANGSCHERAPDLPRQTEPVQ

Target ORF information:

RefSeq Version	XM_011240011.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus epidermal growth factor (Egf), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011240011.2

ORF Insert Sequence:

ATGCCCTGGG GCCGAAGGCC AACCTGGCTG TTGCTCGCCT TCCTGCTGGT GTTTTTAAAG 
ATTAGCATAC TCAGCGTCAC AGCATGGCAG ACCGGGAACT GTCAGCCAGG TCCTCTCGAG 
AGAAGCGAGA GAAGCGGGAC TTGTGCCGGT CCTGCCCCCT TCCTAGTTTT CTCACAAGGA 
AAGAGCATCT CTCGGATTGA CCCAGATGGA ACAAATCACC AGCAATTGGT GGTGGATGCT 
GGCATCTCAG CAGACATGGA TATTCATTAT AAAAAAGAGA GACTCTATTG GGTGGATGTA 
GAAAGACAAG TTTTGCTAAG AGTTTTCCTT AACGGGACAG GACTAGAGAA AGTGTGCAAT 
GTAGAGAGGA AGGTGTCTGG GCTGGCCATA GACTGGATAG ATGATGAAGT TCTCTGGGTA 
GACCAACAGA ACGGAGTCAT CACCGTAACA GATATGACAG GGAAAAATTC CCGAGTTCTT 
CTAAGTTCCT TAAAACATCC GTCAAATATA GCAGTGGATC CAATAGAGAG GTTGATGTTT 
TGGTCTTCAG AGGTGACCGG CAGCCTTCAC AGAGCACACC TCAAAGGTGT TGATGTAAAA 
ACACTGCTGG AGACAGGGGG AATATCGGTG CTGACTCTGG ATGTCCTGGA CAAACGGCTC 
TTCTGGGTTC AGGACAGTGG CGAAGGAAGC CACGCTTACA TTCATTCCTG TGATTATGAG 
GGTGGCTCCG TCCGTCTTAT CAGGCATCAA GCACGGCACA GTTTGTCTTC AATGGCCTTT 
TTTGGTGATC GGATCTTCTA CTCAGTGTTG AAAAGCAAGG CGATTTGGAT AGCCAACAAA 
CACACGGGGA AGGACACGGT CAGGATTAAC CTCCATCCAT CCTTTGTGAC ACCTGGAAAA 
CTGATGGTAG TACACCCTCG TGCACAGCCC AGGACAGAGG ACGCTGCTAA GGATCCTGGT 
GAGTCACTAG ACCCCGAACT TCTCAAACAG AGGGGAAGAC CATGCCGCTT CGGTCTCTGT 
GAGCGAGACC CCAAGTCCCA CTCGAGCGCA TGCGCTGAGG GCTACACGTT AAGCCGAGAC 
CGGAAGTACT GCGAAGATGT CAATGAATGT GCCACTCAGA ATCACGGCTG TACTCTTGGG 
TGTGAAAACA CCCCTGGATC CTATCACTGC ACATGCCCCA CAGGATTTGT TCTGCTTCCT 
GATGGGAAAC AATGTCACGA ACTTGTTTCC TGCCCAGGCA ACGTATCAAA GTGCAGTCAT 
GGCTGTGTCC TGACATCAGA TGGTCCCCGG TGCATCTGTC CTGCAGGTTC AGTGCTTGGG 
AGAGATGGGA AGACTTGCAC TGGTTGTTCA TCGCCTGACA ATGGTGGATG CAGCCAGATC 
TGTCTTCCTC TCAGGCCAGG ATCCTGGGAA TGTGATTGCT TTCCTGGGTA TGACCTACAG 
TCAGACCGAA AGAGCTGTGC AGCTTCAGGA CCACAGCCAC TTTTACTGTT TGCAAATTCC 
CAGGACATCC GACACATGCA TTTTGATGGA ACAGACTACA AAGTTCTGCT CAGCCGGCAG 
ATGGGAATGG TTTTTGCCTT GGATTATGAC CCTGTGGAAA GCAAGATATA TTTTGCACAG 
ACAGCCCTGA AGTGGATAGA GAGGGCTAAT ATGGATGGGT CCCAGCGAGA AAGACTGATC 
ACAGAAGGAG TAGATACGCT TGAAGGTCTT GCCCTGGACT GGATTGGCCG GAGAATCTAC 
TGGACAGACA GTGGGAAGTC TGTTGTTGGA GGGAGCGATC TGAGCGGGAA GCATCATCGA 
ATAATCATCC AGGAGAGAAT CTCGAGGCCG CGAGGAATAG CTGTGCATCC AAGGGCCAGG 
AGACTGTTCT GGACGGACGT AGGGATGTCT CCACGGATTG AAAGCGCTTC CCTTCAAGGT 
TCCGACCGGG TGCTGATAGC CAGCTCCAAT CTACTGGAAC CCAGTGGAAT CACGATTGAC 
TACTTAACAG ACACTTTGTA CTGGTGTGAC ACCAAGAGGT CTGTGATTGA AATGGCCAAT 
CTGGATGGCT CCAAACGCCG AAGACTTATC CAGAACGACG TAGGTCACCC CTTCTCTCTA 
GCCGTGTTTG AGGATCACCT GTGGGTCTCG GATTGGGCTA TCCCATCGGT AATAAGGGTG 
AACAAGAGGA CTGGCCAAAA CAGGGTACGT CTTCAAGGCA GCATGCTGAA GCCCTCGTCA 
CTGGTTGTGG TCCATCCATT GGCAAAACCA GGTGCAGATC CCTGCTTATA CAGGAATGGA 
GGCTGTGAAC ACATCTGCCA AGAGAGCCTG GGCACAGCTC GGTGTTTGTG TCGTGAAGGT 
TTTGTGAAGG CCTGGGATGG GAAAATGTGT CTCCCTCAGG ATTATCCAAT CCTGTCAGGT 
GAAAATGCTG ATCTTAGTAA AGAGGTGACA TCACTGAGCA ACTCCACTCA GGCTGAAGTA 
CCAGACGATG ATGGGACAGA ATCTTCCACA CTAGTGGCTG AAATCATGGT GTCAGGCATG 
AACTATGAAG ATGACTGTGG TCCCGGGGGG TGTGGAAGCC ATGCTCGATG CGTTTCAGAC 
GGAGAGACTG CTGAGTGTCA GTGTCTGAAA GGGTTTGCCA GGGATGGAAA CCTGTGTTCT 
GATATAGATG AGTGTGTGCT GGCTAGATCG GACTGCCCCA GCACCTCGTC CAGGTGCATC 
AACACTGAAG GTGGCTACGT CTGCAGATGC TCAGAAGGCT ACGAAGGAGA CGGGATCTCC 
TGTTTCGACT CTACCGCACC CTCTCTCCTT GGGGAAGATG GCCACCATTT GGACCGAAAT 
AGTTATCCAG GATGCCCATC CTCATATGAT GGATACTGCC TCAATGGTGG CGTGTGCATG 
CATATTGAAT CACTGGACAG CTACACATGC AACTGTGTTA TTGGCTATTC TGGGGATCGA 
TGCCAGACTC GAGACCTACG ATGGTGGGAA CTGCGTCATG CTGGCTACGG GCAGAAGCAT 
GACATCATGG TGGTGGCTGT CTGCATGGTG GCACTGGTCC TGCTGCTCGT CTTGGGGATG 
TGGGGGACTT ACTACTACAG GACTCGGAAG CAGCTATCAA ACCCCCCAAA GAACCCTTGT 
GATGAGCCAA GCGGAAGTGT GAGCAGCAGC GGACCCAACA GCAGCAGCGG GGCAGCTGTG 
GCTTCTTGTC CCCAACCTTG GTTTGTGGTC CTAGAGAAAC ACCAAGACCC CAAGAATGGG 
AGTCTGCCTG CGGATGGTAC GAATGGTGCA GTAGTAGATG CTGGCCTGTC TCCCTCCCTG 
CAGCTCGGGT CAGTGCATCT GACTTCATGG AGACAGAAGC CCCACATAGA TGGAATGGGC 
ACAGGGCAAA GCTGCTGGAT TCCACCATCA AGTGACAGAG GACCCCAGGA AATAGAGGGA 
AACTCCCACC TACCCTCCTA CAGACCTGTG GGGCCGGAGA AGCTGCATTC TCTCCAGTCA 
GCTAATGGAT CGTGTCACGA AAGGGCTCCA GACCTGCCAC GGCAGACAGA GCCAGTTCAG 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	Egf
Entrez Gene ID	13645
Full Name	epidermal growth factor
Synonyms	AI790464
General protein information	Preferred Names epidermal growth factor Names pro-epidermal growth factor Pro-epidermal growth factor precursor (EGF)
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 3 Map Location: 3 G3\|3 58.5 cM
Summary	This gene encodes epidermal growth factor (EGF), the founding member of the EGF family of growth factors that are implicated in cell proliferation and differentiation. The encoded protein can localize to the membrane and function in juxtacrine signaling or undergo proteolytic processing to generate a soluble form of the hormone. Mice lacking the encoded protein do not exhibit an abnormal phenotype but transgenic mice overexpressing the encoded protein exhibit hypospermatogenesis. [provided by RefSeq, Jul 2016].

Homo sapiens (human)	EGF	NP_001954.2
Bos taurus (cattle)	EGF	XP_002688149.2
Mus musculus (house mouse)	Egf	NP_034243.2
	EGF	XP_001088957.1
Rattus norvegicus (Norway rat)	Egf	NP_036974.1
Canis lupus familiaris (dog)	EGF	NP_001003094.1
Danio rerio (zebrafish)	egf	NP_991294.1
Pan troglodytes (chimpanzee)	EGF	XP_517395.2
Gallus gallus (chicken)	EGF	NP_001001292.1
Xenopus tropicalis (tropical clawed frog)	egf	NP_001106478.1

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

Egf cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

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ORF Insert Sequence:

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ORF Insert Sequence:

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ORF Insert Sequence:

Target ORF information:

Target ORF information:

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Target ORF information:

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