Edn3 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Edn3
Entrez Gene ID	13616
Full Name	endothelin 3
Synonyms	ET-3,PPET3,ls,tmgc48
General protein information	Preferred Names endothelin 3 Names endothelin-3 preproendothelin-3
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 2 Map Location: 2 H4\|2 98.1 cM
Summary	This gene is a member of the endothelin family whose members encode proteins that act on G protein-coupled receptors. Endothelins are produced as large prepropolypeptide precursors that undergo a first cleavage by a subtilisin serine protease to form an inactive intermediate, which in turn is cleaved again by endothelin-converting enzyme 1 (ECE-1) to yield the active 21 amino acid peptide. This gene encodes a protein which is expressed in neural crest cells (NCC), binds to endothelin receptor b (Ednrb) and plays an essential role in the development of NCC-derived cell lineages including melanocytes and enteric neurons. Mutations in this gene are associated with terminal aganglionosis and white spotted coat in mice and Hirschsprung's disease and Waardenburg syndrome in humans. [provided by RefSeq, Apr 2013].

mRNA and Protein(s)

mRNA	Protein	Name
NM_007903.5	NP_031929.1	endothelin-3 preproprotein

Pathways from BioSystems

Homologies

Homo sapiens (human)	EDN3	NP_996915.1
Pan troglodytes (chimpanzee)	EDN3	XP_525373.2
Bos taurus (cattle)	EDN3	NP_001095449.1
Mus musculus (house mouse)	Edn3	NP_031929.1
Rattus norvegicus (Norway rat)	Edn3	NP_001071118.1
Canis lupus familiaris (dog)	EDN3	NP_001002942.1
Gallus gallus (chicken)	EDN3	XP_001231488.3

Gene Ontology
Bibliography

Related articles in PubMed

Transcription and Signaling Regulators in Developing Neuronal Subtypes of Mouse and Human Enteric Nervous System.
Memic F, Knoflach V, Morarach K, Sadler R, Laranjeira C, Hjerling-Leffler J, Sundstr?m E, Pachnis V, Marklund U
Gastroenterology(2017 Oct)

Differentiation of Mouse Enteric Nervous System Progenitor Cells Is Controlled by Endothelin 3 and Requires Regulation of Ednrb by SOX10 and ZEB2.
Watanabe Y, Stanchina L, Lecerf L, Gacem N, Conidi A, Baral V, Pingault V, Huylebroeck D, Bondurand N
Gastroenterology152(5)1139-1150.e4(2017 Apr)

Endothelin-3 stimulates cell adhesion and cooperates with ?1-integrins during enteric nervous system ontogenesis.
Gazquez E, Watanabe Y, Broders-Bondon F, Paul-Gilloteaux P, Heysch J, Baral V, Bondurand N, Dufour S
Scientific reports637877(2016 Dec)

Mouse models of Hirschsprung disease and other developmental disorders of the enteric nervous system: Old and new players.
Bondurand N, Southard-Smith EM
Developmental biology417(2)139-57(2016 09)

Mutations in the endothelin receptor type A cause mandibulofacial dysostosis with alopecia.
Gordon CT, Weaver KN, Zechi-Ceide RM, Madsen EC, Tavares AL, Oufadem M, Kurihara Y, Adameyko I, Picard A, Breton S, Pierrot S, Biosse-Duplan M, Voisin N, Masson C, Bole-Feysot C, Nitschk? P, Delrue MA, Lacombe D, Guion-Almeida ML, Moura PP, Garib DG, Munnich A, Ernfors P, Hufnagel RB, Hopkin RJ, Kurihara H, Saal HM, Weaver DD, Katsanis N, Lyonnet S, Golzio C, Clouthier DE, Amiel J
American journal of human genetics96(4)519-31(2015 Apr)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Control of differentiation of mouse enteric nervous system progenitor cells by EDN3 requires regulation of Ednrb expression by SOX10 and ZEB2.
Title: Differentiation of Mouse Enteric Nervous System Progenitor Cells Is Controlled by Endothelin 3 and Requires Regulation of Ednrb by SOX10 and ZEB2.

colonic migrating motor complexes occur in mice lacking the endothelin-3 gene
Title: Can colonic migrating motor complexes occur in mice lacking the endothelin-3 gene?

Enterocolitis causes profound lymphoid depletion in endothelin receptor B- and endothelin 3-null mouse models of Hirschsprung-associated enterocolitis.
Title: Enterocolitis causes profound lymphoid depletion in endothelin receptor B- and endothelin 3-null mouse models of Hirschsprung-associated enterocolitis.

Endothelin3 signaling has a critical role in development of UV-induced melanoma in a mouse model
Title: Novel UV-induced melanoma mouse model dependent on Endothelin3 signaling.

changes in spinal afferent innervation and visceral pain transmission from the aganglionic rectum in ls/ls mice with deletions of the endothelin-3 gene
Title: Loss of visceral pain following colorectal distension in an endothelin-3 deficient mouse model of Hirschsprung's disease.

The following Edn3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Edn3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu01022	NM_007903.5 Latest version!	Mus musculus endothelin 3 (Edn3), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$139.30 $199.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Edn3 ( NM_007903.5 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_031929.1 Mus musculus endothelin 3 (Edn3), mRNA.

Price & Availability↑

CloneID	OMu01022
Clone ID Related Accession (Same CDS sequence)	NM_007903.5
Accession Version	NM_007903.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 645bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-22
Organism	Mus musculus(house mouse)
Product	endothelin-3 preproprotein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL844489.6, AK079150.1 and AK049775.1. On Feb 5, 2016 this sequence version replaced NM_007903.4. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK049775.1, BC137727.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849376 [ECO:0000348] ##Evidence-Data-END##

ATGGAGCCGG GGCTGTGGCT CCTTCTCGGG CTCACAGTGA CCTCCGCTGC AGGACTTGTG 
CCTTGCCCCC AGTCTGGGGA CTCTGGCAGA GCCAGTGTGT CCCAGGGTCC CCCTGAAGCT 
GGATCAGAGA GGGGCTGTGA AGAGACTGTG GCTGGCCCTG GTGAGAGGAT TGTGTCCCCA 
ACAGTTGCAC TGCCTGCACA GCCTGAAAGC GCTGGGCAGG AACGGGCACC AGGCAGGTCT 
GGGAAACAAG AGGACAAGGG GCTGCCTGCA CACCACCGCC CTCGCCGCTG CACGTGCTTC 
ACTTACAAGG ACAAGGAGTG TGTCTACTAT TGCCACCTGG ACATCATCTG GATTAACACT 
CCTGAACAGA CTGTGCCCTA TGGACTGTCC AACTACAGAG AAAGCCTTCG GGGAAAGAGG 
TCCTTGGGGC CAGTTCCAGA AAGCTCCCAG CCTTCTCCGT GGACACGCTT GCGTTGTACT 
TGTATGGGGG CGGATGACAA GGCCTGTGCA CACTTCTGTG CACGCACCAG AGATGTCACC 
AGTTATTCCG GGAGAGCAGA AAGGCCAGCT GCAGAAGAGA TGCGGGAGAC TGGAGGCCCA 
CGTCAAAGGT TGATGTCAAG GACAGATAAA GCCCACCGGC CTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_031929.1
CDS	349..993
Translation	MEPGLWLLLGLTVTSAAGLVPCPQSGDSGRASVSQGPPEAGSERGCEETVAGPGERIVSPTVALPAQPESAGQERAPGRSGKQEDKGLPAHHRPRRCTCFTYKDKECVYYCHLDIIWINTPEQTVPYGLSNYRESLRGKRSLGPVPESSQPSPWTRLRCTCMGADDKACAHFCARTRDVTSYSGRAERPAAEEMRETGGPRQRLMSRTDKAHRP

Target ORF information:

RefSeq Version	NM_007903.5
Organism	Mus musculus(house mouse)
Definition	Mus musculus endothelin 3 (Edn3), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_007903.5

ORF Insert Sequence:

ATGGAGCCGG GGCTGTGGCT CCTTCTCGGG CTCACAGTGA CCTCCGCTGC AGGACTTGTG 
CCTTGCCCCC AGTCTGGGGA CTCTGGCAGA GCCAGTGTGT CCCAGGGTCC CCCTGAAGCT 
GGATCAGAGA GGGGCTGTGA AGAGACTGTG GCTGGCCCTG GTGAGAGGAT TGTGTCCCCA 
ACAGTTGCAC TGCCTGCACA GCCTGAAAGC GCTGGGCAGG AACGGGCACC AGGCAGGTCT 
GGGAAACAAG AGGACAAGGG GCTGCCTGCA CACCACCGCC CTCGCCGCTG CACGTGCTTC 
ACTTACAAGG ACAAGGAGTG TGTCTACTAT TGCCACCTGG ACATCATCTG GATTAACACT 
CCTGAACAGA CTGTGCCCTA TGGACTGTCC AACTACAGAG AAAGCCTTCG GGGAAAGAGG 
TCCTTGGGGC CAGTTCCAGA AAGCTCCCAG CCTTCTCCGT GGACACGCTT GCGTTGTACT 
TGTATGGGGG CGGATGACAA GGCCTGTGCA CACTTCTGTG CACGCACCAG AGATGTCACC 
AGTTATTCCG GGAGAGCAGA AAGGCCAGCT GCAGAAGAGA TGCGGGAGAC TGGAGGCCCA 
CGTCAAAGGT TGATGTCAAG GACAGATAAA GCCCACCGGC CTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Transcription and Signaling Regulators in Developing Neuronal Subtypes of Mouse and Human Enteric Nervous System.
Gastroenterology(2017 Oct)
Memic F,Knoflach V,Morarach K,Sadler R,Laranjeira C,Hjerling-Leffler J,Sundstr?m E,Pachnis V,Marklund U

Differentiation of Mouse Enteric Nervous System Progenitor Cells Is Controlled by Endothelin 3 and Requires Regulation of Ednrb by SOX10 and ZEB2.
Gastroenterology152(5)1139-1150.e4(2017 Apr)
Watanabe Y,Stanchina L,Lecerf L,Gacem N,Conidi A,Baral V,Pingault V,Huylebroeck D,Bondurand N

Endothelin-3 stimulates cell adhesion and cooperates with ?1-integrins during enteric nervous system ontogenesis.
Scientific reports637877(2016 Dec)
Gazquez E,Watanabe Y,Broders-Bondon F,Paul-Gilloteaux P,Heysch J,Baral V,Bondurand N,Dufour S

Mouse models of Hirschsprung disease and other developmental disorders of the enteric nervous system: Old and new players.
Developmental biology417(2)139-57(2016 09)
Bondurand N,Southard-Smith EM

Mutations in the endothelin receptor type A cause mandibulofacial dysostosis with alopecia.
American journal of human genetics96(4)519-31(2015 Apr)
Gordon CT,Weaver KN,Zechi-Ceide RM,Madsen EC,Tavares AL,Oufadem M,Kurihara Y,Adameyko I,Picard A,Breton S,Pierrot S,Biosse-Duplan M,Voisin N,Masson C,Bole-Feysot C,Nitschk? P,Delrue MA,Lacombe D,Guion-Almeida ML,Moura PP,Garib DG,Munnich A,Ernfors P,Hufnagel RB,Hopkin RJ,Kurihara H,Saal HM,Weaver DD,Katsanis N,Lyonnet S,Golzio C,Clouthier DE,Amiel J

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Edn3 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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