Ear1 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Ear1
Entrez Gene ID	13586
Full Name	eosinophil-associated, ribonuclease A family, member 1
Synonyms	EAR-1,ECP 1,Raf1
General protein information	Preferred Names eosinophil-associated, ribonuclease A family, member 1 Names eosinophil cationic protein 1 RNase 3-1 eosinophil secondary granule ribonuclease 1 eosinophil-associated ribonuclease 1 ribonuclease 3-1 ribonuclease A f1
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 14 Map Location: 14\|14 C1

mRNA and Protein(s)

mRNA	Protein	Name
NM_007894.2	NP_031920.1	eosinophil cationic protein 1 precursor

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Ear1	NP_031920.1
Mus musculus (house mouse)	Ear6	NP_444341.1
Mus musculus (house mouse)	Ear10	NP_444342.1
Mus musculus (house mouse)	Ear2	NP_031921.1

Gene Ontology
Bibliography

Related articles in PubMed

The transcription factor XBP1 is selectively required for eosinophil differentiation.
Bettigole SE, Lis R, Adoro S, Lee AH, Spencer LA, Weller PF, Glimcher LH
Nature immunology16(8)829-37(2015 Aug)

Abnormal Mammary Development in 129:STAT1-Null Mice is Stroma-Dependent.
Chen JQ, Mori H, Cardiff RD, Trott JF, Hovey RC, Hubbard NE, Engelberg JA, Tepper CG, Willis BJ, Khan IH, Ravindran RK, Chan SR, Schreiber RD, Borowsky AD
PloS one10(6)e0129895(2015)

Comparative genomic analysis of eutherian ribonuclease A genes.
Premzl M
Molecular genetics and genomics : MGG289(2)161-7(2014 Apr)

Neonatal oxygen increases sensitivity to influenza A virus infection in adult mice by suppressing epithelial expression of Ear1.
O'Reilly MA, Yee M, Buczynski BW, Vitiello PF, Keng PC, Welle SL, Finkelstein JN, Dean DA, Lawrence BP
The American journal of pathology181(2)441-51(2012 Aug)

The ribonuclease A superfamily of mammals and birds: identifying new members and tracing evolutionary histories.
Cho S, Beintema JJ, Zhang J
Genomics85(2)208-20(2005 Feb)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

These findings demonstrate that novel epithelial expression of Ear1 functions to limit influenza A virus infection.
Title: Neonatal oxygen increases sensitivity to influenza A virus infection in adult mice by suppressing epithelial expression of Ear1.

The following Ear1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Ear1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu21869	NM_007894.2 Latest version!	Mus musculus eosinophil-associated, ribonuclease A family, member 1 (Ear1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$69.30 $99.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Ear1 ( NM_007894.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_031920.1 Mus musculus eosinophil-associated, ribonuclease A family, member 1 (Ear1), mRNA.

Price & Availability↑

CloneID	OMu21869
Clone ID Related Accession (Same CDS sequence)	NM_007894.2
Accession Version	NM_007894.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 468bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-18
Organism	Mus musculus(house mouse)
Product	eosinophil cationic protein 1 precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AY316149.1. On Aug 14, 2003 this sequence version replaced NM_007894.1. ##Evidence-Data-START## Transcript exon combination :: AY316149.1, BC117060.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849375, SAMN00849381 [ECO:0000348] ##Evidence-Data-END##

ATGGGTCCGA AGCTGCTTGA GTCCCGACTT TGTCTCCTGC TGCTGCTAGG ACTTGTCCTA 
ATGCTTGCCT CATGCCTGGG TCAAACCCCT TCCCAGAAGT TTGCCATCCA GCATATCAAT 
AATAATACCA ACCTCCAATG TAATGTTGAA ATGATGCGTA TTAACAGGGC TAGAAGAACA 
TGTAAGGGCT TAAATACTTT TCTTCATACA AGTTTTGCTA ATGCTGTTGG TGTGTGTGGA 
AATCCAAGTG GCTTGTGCAG TGACAAGAGA AGTCAAAACT GTCATAATAG TTCATCTCGG 
GTACATATAA CTGTCTGTAA CATCACCAGT CGGGCAACAA ATTATACCCA ATGCAGATAC 
CAATCAAGAA GATCATTGGA GTACTACACA GTTGCCTGTG ACCCCAGAAC TCCACAGGAC 
AGTCCCATGT ATCCAGTGGT TCCAGTTCAC TTGGATGGGA CATTTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_031920.1
CDS	69..536
Translation	MGPKLLESRLCLLLLLGLVLMLASCLGQTPSQKFAIQHINNNTNLQCNVEMMRINRARRTCKGLNTFLHTSFANAVGVCGNPSGLCSDKRSQNCHNSSSRVHITVCNITSRATNYTQCRYQSRRSLEYYTVACDPRTPQDSPMYPVVPVHLDGTF

Target ORF information:

RefSeq Version	NM_007894.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus eosinophil-associated, ribonuclease A family, member 1 (Ear1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_007894.2

ORF Insert Sequence:

ATGGGTCCGA AGCTGCTTGA GTCCCGACTT TGTCTCCTGC TGCTGCTAGG ACTTGTCCTA 
ATGCTTGCCT CATGCCTGGG TCAAACCCCT TCCCAGAAGT TTGCCATCCA GCATATCAAT 
AATAATACCA ACCTCCAATG TAATGTTGAA ATGATGCGTA TTAACAGGGC TAGAAGAACA 
TGTAAGGGCT TAAATACTTT TCTTCATACA AGTTTTGCTA ATGCTGTTGG TGTGTGTGGA 
AATCCAAGTG GCTTGTGCAG TGACAAGAGA AGTCAAAACT GTCATAATAG TTCATCTCGG 
GTACATATAA CTGTCTGTAA CATCACCAGT CGGGCAACAA ATTATACCCA ATGCAGATAC 
CAATCAAGAA GATCATTGGA GTACTACACA GTTGCCTGTG ACCCCAGAAC TCCACAGGAC 
AGTCCCATGT ATCCAGTGGT TCCAGTTCAC TTGGATGGGA CATTTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The transcription factor XBP1 is selectively required for eosinophil differentiation.
Nature immunology16(8)829-37(2015 Aug)
Bettigole SE,Lis R,Adoro S,Lee AH,Spencer LA,Weller PF,Glimcher LH

Abnormal Mammary Development in 129:STAT1-Null Mice is Stroma-Dependent.
PloS one10(6)e0129895(2015)
Chen JQ,Mori H,Cardiff RD,Trott JF,Hovey RC,Hubbard NE,Engelberg JA,Tepper CG,Willis BJ,Khan IH,Ravindran RK,Chan SR,Schreiber RD,Borowsky AD

Comparative genomic analysis of eutherian ribonuclease A genes.
Molecular genetics and genomics : MGG289(2)161-7(2014 Apr)
Premzl M

Neonatal oxygen increases sensitivity to influenza A virus infection in adult mice by suppressing epithelial expression of Ear1.
The American journal of pathology181(2)441-51(2012 Aug)
O'Reilly MA,Yee M,Buczynski BW,Vitiello PF,Keng PC,Welle SL,Finkelstein JN,Dean DA,Lawrence BP

The ribonuclease A superfamily of mammals and birds: identifying new members and tracing evolutionary histories.
Genomics85(2)208-20(2005 Feb)
Cho S,Beintema JJ,Zhang J