Atn1 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Atn1
Entrez Gene ID	13498
Full Name	atrophin 1
Synonyms	Atr1,Drpla,atrophin-1
General protein information	Preferred Names atrophin 1 Names atrophin-1 dentatorubral pallidoluysian atrophy dentatorubral-pallidoluysian atrophy protein homolog
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 6 Map Location: 6 F2\|6 59.17 cM

mRNA and Protein(s)

mRNA	Protein	Name
NM_007881.4	NP_031907.2	atrophin-1

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Atn1	NP_031907.2
Gallus gallus (chicken)	ATN1	XP_425518.4
Canis lupus familiaris (dog)	ATN1	XP_003640006.1
Bos taurus (cattle)	ATN1	NP_001192677.1
Rattus norvegicus (Norway rat)	LOC100911672	XP_003750706.1
Pan troglodytes (chimpanzee)	ATN1	NP_001029337.1
Rattus norvegicus (Norway rat)	Atn1	NP_058924.2
Homo sapiens (human)	ATN1	NP_001007027.1
	ATN1	XP_001118307.2

Gene Ontology
Bibliography

Related articles in PubMed

Post-transcriptional regulation of mouse neurogenesis by Pumilio proteins.
Zhang M, Chen D, Xia J, Han W, Cui X, Neuenkirchen N, Hermes G, Sestan N, Lin H
Genes & development(2017 Aug)

High-throughput discovery of novel developmental phenotypes.
Dickinson ME, Flenniken AM, Ji X, Teboul L, Wong MD, White JK, Meehan TF, Weninger WJ, Westerberg H, Adissu H, Baker CN, Bower L, Brown JM, Caddle LB, Chiani F, Clary D, Cleak J, Daly MJ, Denegre JM, Doe B, Dolan ME, Edie SM, Fuchs H, Gailus-Durner V, Galli A, Gambadoro A, Gallegos J, Guo S, Horner NR, Hsu CW, Johnson SJ, Kalaga S, Keith LC, Lanoue L, Lawson TN, Lek M, Mark M, Marschall S, Mason J, McElwee ML, Newbigging S, Nutter LM, Peterson KA, Ramirez-Solis R, Rowland DJ, Ryder E, Samocha KE, Seavitt JR, Selloum M, Szoke-Kovacs Z, Tamura M, Trainor AG, Tudose I, Wakana S, Warren J, Wendling O, West DB, Wong L, Yoshiki A, , , , , , , , , MacArthur DG, Tocchini-Valentini GP, Gao X, Flicek P, Bradley A, Skarnes WC, Justice MJ, Parkinson HE, Moore M, Wells S, Braun RE, Svenson KL, de Angelis MH, Herault Y, Mohun T, Mallon AM, Henkelman RM, Brown SD, Adams DJ, Lloyd KC, McKerlie C, Beaudet AL, Bu?an M, Murray SA
Nature537(7621)508-514(2016 09)

Functional interactions between Fat family cadherins in tissue morphogenesis and planar polarity.
Saburi S, Hester I, Goodrich L, McNeill H
Development (Cambridge, England)139(10)1806-20(2012 May)

C-terminal deletion of the atrophin-1 protein results in growth retardation but not neurodegeneration in mice.
Yu J, Ying M, Zhuang Y, Xu T, Han M, Wu X, Xu R
Developmental dynamics : an official publication of the American Association of Anatomists238(10)2471-8(2009 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

These results support the model that poly-Q expanded Atrophin-1 proteins cause dentatorubral-pallidoluysian atrophy in a manner independent of any functional interaction with wild-type Atrophin-1 proteins.
Title: C-terminal deletion of the atrophin-1 protein results in growth retardation but not neurodegeneration in mice.

Fat1 and Atrs 1,2 act in concert after vascular injury but show further that distinct Atr isoforms have disparate effects on VSMC directional migration.
Title: Atrophin proteins interact with the Fat1 cadherin and regulate migration and orientation in vascular smooth muscle cells.

Atrophin-1 and the short form of Atrophin-2 can act as potent and evolutionarily conserved transcriptional activators.
Title: Functional architecture of atrophins.

TLX interacts with atrophin1 (Atn1), a corepressor that is involved in human neurodegenerative dentatorubral-pallidoluysian atrophy (DRPLA) and that is essential for development of multiple tissues.
Title: Nuclear receptor TLX prevents retinal dystrophy and recruits the corepressor atrophin1.

mutant atropphin with polyglutamine expansion does not simply function in a dominant negative manner
Title: Sodium butyrate ameliorates histone hypoacetylation and neurodegenerative phenotypes in a mouse model for DRPLA.

The following Atn1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Atn1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu00972	NM_007881.4 Latest version!	Mus musculus atrophin 1 (Atn1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$692.30 $989.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Atn1 ( NM_007881.4 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_031907.2 Mus musculus atrophin 1 (Atn1), mRNA.

Price & Availability↑

CloneID	OMu00972
Clone ID Related Accession (Same CDS sequence)	NM_007881.4
Accession Version	NM_007881.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3528bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-23
Organism	Mus musculus(house mouse)
Product	atrophin-1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BC050920.1 and BC053051.1. On or before May 4, 2006 this sequence version replaced XM_981322.1, NM_007881.3. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC050920.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END##

ATGAAGACAC GACAGAATAA AGACTCGATG TCAATGAGGA GTGGACGGAA GAAAGAGGCC 
CCCGGGCCCC GGGAAGAGCT GAGATCAAGG GGCCGGGCCT CCCCTGGAGG GGTCAGCACA 
TCCAGCAGTG ATGGCAAAGC TGAGAAGTCC AGGCAGACAG CCAAGAAGGC CCGGATAGAG 
GAGCCCTCTG CCCCAAAGGC CAGCAAGCAG GGCCGGAGCG AGGAGATCTC AGAGAGTGAG 
AGCGAGGAGA CCAGTGCGCC CAAAAAGACC AAAACCGAGC AGGAGCTCCC TCGCCCGCAG 
TCTCCCTCGG ATCTGGACAG CTTGGATGGG CGCAGCATTA ACGATGACGG CAGCAGCGAC 
CCTAGAGATA TAGACCAGGA CAACCGAAGC ACATCCCCCA GCATCTACAG CCCGGGCAGC 
GTGGAAAATG ACTCGGACTC ATCCTCTGGC CTGTCCCAGG GCCCCGCCCG CCCCTACCAC 
CCACCTCCAC TCTTCCCTCC TTCCCCTCCA CCACCAGACA GCACTCCCCG ACAGCCAGAG 
TCTGGCTTTG AACCTCATCC TTCTGTGCCG CCTACTGGAT ATCATGCTCC GATGGAGCCC 
CCCACATCGA GATTATTCCA GGGCCCACCA CCTGGAGCTC CTCCCACACA CCCACAGCTC 
TACCCTGGGA ATGCTAGTGG AGGTGTTTTA TCTGGACCCC CCATGGGTCC CAAAGGGGGA 
GCCGCTGCCT CCTCAGTGGG TGCCCCTAGC GGAGGCAAGC AACACCCCCC ACCCACTACC 
CCAATTCCAA TATCAAGTTC TGGGGCCAGT GGTGCTCCTC CAGCAAAGCC ACCCAGTGCT 
CCAGTGGGTG GTGGGAGCTT ACCTTCTGCA CCACCACCAG CTTCTTTCCC CCATGTGACA 
CCAAACCTGC CTCCTCCACC TGCCCTGAGA CCCCTCAACA ATGCCTCAGC CTCTCCTCCT 
GGCATGGGGG CTCAGCCAAT CCCTGGGCAT CTGCCCTCTC CCCATGCCAT GGGGCAGGGC 
ATGAGTGGAC TTCCTCCTGG CCCAGAGAAG GGTCCAACCC TGGCCCCTTC TCCCCACCCT 
TTGCCCCCAG CTTCTTCCTC TGCCCCTGGG CCTCCAATGC GATATCCATA TTCATCCTCC 
AGTAGCTCTG CCGCAGCCTC TTCTAGTTCC TCCTCCTCCT CTGCCTCCCA GTACCCTGCT 
TCCCAGGCCC TGCCCAGTTA TCCTCATTCC TTCCCCCCAC CAACTAGTAT GTCTGTCTCT 
AATCAGCCAC CCAAGTACAC CCAGCCTTCT CTCCCATCCC AAGCTGTGTG GAGCCAGGGT 
CCACCTCCTC CTCCTCCCTA TGGCCGCCTC TTGGCCAACA ACAACACCCA TCCAGGCCCT 
TTCCCTCCTA CTGGGGGTCA ATCTACAGCC CACCCAGCAG CCCCTACACA TCACCATCAC 
CAGCAGCAGC CACAGCAACA ACATCATCAT GGAAACTCTG GGCCCCCTCC ACCCGGAGCG 
TATCCTCACC CTCTAGAGAG CAGTAACTCC CATCATGCAC ACCCTTACAA CATGTCACCC 
TCCCTGGGGT CTTTAAGGCC CTACCCCCCA GGGCCAGCAC ACCTGCCTCC ACCTCATGGC 
CAGGTGTCCT ATAACCAAGC AGGTCCCAAT GGTCCCCCAG TTTCTTCTTC CAACTCTTCC 
GGGTCTTCCT CTCAAGCCTC CTATTCATGT TCACACCCCT CTTCATCCCA GGGCCCCCAA 
GGAGCATCCT ACCCCTTCCC ACCAGTCCCT CCAGTCACCA CCTCCTCAGC TACCCTTTCC 
ACTGTCATCG CCACCGTGGC TTCCTCGCCA GCAGGCTACA AAACAGCTTC GCCACCTGGG 
CCCCCTCAGT ACAGCAAGAG AGCCCCATCC CCAGGGTCCT ACAAGACAGC CACCCCGCCT 
GGATACAAAC CGGGGTCACC ACCCTCCTTC AGAACAGGGA CCCCACCCGG CTATCGAGGC 
ACCTCTCCGC CAGCAGGCCC AGGGACCTTC AAACCAGGTT CACCGACCGT GGGGCCGGGG 
CCCCTGCCAC CCGCGGGGCC TTCAAGTTTG TCATCTCTGC CTCCGCCACC TGCGGCCCCG 
ACTACAGGGC CGCCCCTGAC CGCCACGCAG ATCAAACAGG AGCCGGCGGA AGAGTATGAA 
CCTCCCGAGA GTCCGGTGCC TCCGGCCCGC AGCCCCTCGC CCCCTCCCAA GGTGGTGGAC 
GTGCCCAGCC ATGCCAGCCA GTCAGCCAGG TTCAATAAGC ACTTGGACCG CGGCTTCAAC 
TCGTGCGCGC GCAGCGACCT GTACTTCGTG CCGCTGGAGG GCTCCAAGCT GGCCAAGAAG 
CGCGCGGACC TGGTGGAGAA AGTGCGGCGC GAGGCCGAGC AGCGCGCGCG CGAGGAGAAA 
GAGCGCGAGC GCGAGCGGGA ACGCGAAAAG GAGCGCGAGC GCGAGAAAGA GCGCGAGCTG 
GAGCGCAGTG TGAAACTGGC CCAGGAGGGC CGTGCTCCAG TGGAGTGCCC ATCTCTGGGT 
CCAGTGCCCC ATCGGCCTCC CTTTGAGCCT GGCAGCGCTG TGGCTACAGT GCCCCCTTAC 
CTGGGTCCTG ATACTCCGGC CTTGCGCACT CTCAGTGAAT ACGCCCGACC TCATGTCATG 
TCTCCTGGCA ATCGCAACCA CCCATTCTAT GTGCCCTTGG GGGCAGTGGA CCCGGGGCTT 
CTGGGTTACA ATGTCCCAGC CCTGTACAGC AGCGACCCAG CTGCCCGAGA ACGGGAGCGG 
GAAGCCCGTG AACGTGACCT CCGTGACCGG CTCAAGCCTG GCTTTGAGGT GAAACCTAGT 
GAGCTGGAAC CCCTACATGG GGTTCCCGGG CCAGGCCTGG ATCCCTTCCC CCGACACGGG 
GGCCTGGCTC TACAGCCCGG GCCACCTGGC CTGCATCCTT TCCCTTTTCA TCCGAGCCTG 
GGGCCCCTGG AACGAGAACG GCTAGCGCTG GCAGCTGGGC CAGCCTTGCG TCCTGACATG 
TCTTATGCTG AGCGGTTGGC AGCTGAAAGG CAGCATGCAG AAAGGGTGGC AGCCCTGGGC 
AATGATCCAC TAGCCCGGCT GCAGATGCTC AACGTGACTC CCCATCACCA CCAGCACTCC 
CACATCCACT CTCACCTTCA CCTGCACCAG CAGGATGCTA TCCACGCAGC CTCTGCCTCG 
GTGCACCCTC TCATTGACCC CCTGGCCTCA GGGTCTCACC TTACCCGGAT CCCCTACCCA 
GCTGGGACCC TCCCCAACCC CCTTCTTCCT CACCCTCTGC ACGAGAACGA AGTTCTTCGT 
CACCAGCTTT TTGCTGCCCC TTACCGGGAC CTGCCGGCCT CCCTTTCTGC TCCAATGTCA 
GCGGCTCATC AGCTGCAGGC CATGCACGCG CAGTCAGCTG AGCTGCAGCG CTTGGCGCTG 
GAACAGCAGC AGTGGCTACA TGCTCATCAC CCATTGCACA GCGTGCCACT ACCTGCCCAG 
GAAGACTACT ACAGTCACCT GAAGAAGGAG AGTGACAAGC CGCTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_031907.2
CDS	341..3868
Translation	MKTRQNKDSMSMRSGRKKEAPGPREELRSRGRASPGGVSTSSSDGKAEKSRQTAKKARIEEPSAPKASKQGRSEEISESESEETSAPKKTKTEQELPRPQSPSDLDSLDGRSINDDGSSDPRDIDQDNRSTSPSIYSPGSVENDSDSSSGLSQGPARPYHPPPLFPPSPPPPDSTPRQPESGFEPHPSVPPTGYHAPMEPPTSRLFQGPPPGAPPTHPQLYPGNASGGVLSGPPMGPKGGAAASSVGAPSGGKQHPPPTTPIPISSSGASGAPPAKPPSAPVGGGSLPSAPPPASFPHVTPNLPPPPALRPLNNASASPPGMGAQPIPGHLPSPHAMGQGMSGLPPGPEKGPTLAPSPHPLPPASSSAPGPPMRYPYSSSSSSAAASSSSSSSSASQYPASQALPSYPHSFPPPTSMSVSNQPPKYTQPSLPSQAVWSQGPPPPPPYGRLLANNNTHPGPFPPTGGQSTAHPAAPTHHHHQQQPQQQHHHGNSGPPPPGAYPHPLESSNSHHAHPYNMSPSLGSLRPYPPGPAHLPPPHGQVSYNQAGPNGPPVSSSNSSGSSSQASYSCSHPSSSQGPQGASYPFPPVPPVTTSSATLSTVIATVASSPAGYKTASPPGPPQYSKRAPSPGSYKTATPPGYKPGSPPSFRTGTPPGYRGTSPPAGPGTFKPGSPTVGPGPLPPAGPSSLSSLPPPPAAPTTGPPLTATQIKQEPAEEYEPPESPVPPARSPSPPPKVVDVPSHASQSARFNKHLDRGFNSCARSDLYFVPLEGSKLAKKRADLVEKVRREAEQRAREEKEREREREREKEREREKERELERSVKLAQEGRAPVECPSLGPVPHRPPFEPGSAVATVPPYLGPDTPALRTLSEYARPHVMSPGNRNHPFYVPLGAVDPGLLGYNVPALYSSDPAAREREREARERDLRDRLKPGFEVKPSELEPLHGVPGPGLDPFPRHGGLALQPGPPGLHPFPFHPSLGPLERERLALAAGPALRPDMSYAERLAAERQHAERVAALGNDPLARLQMLNVTPHHHQHSHIHSHLHLHQQDAIHAASASVHPLIDPLASGSHLTRIPYPAGTLPNPLLPHPLHENEVLRHQLFAAPYRDLPASLSAPMSAAHQLQAMHAQSAELQRLALEQQQWLHAHHPLHSVPLPAQEDYYSHLKKESDKPL

Target ORF information:

RefSeq Version	NM_007881.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus atrophin 1 (Atn1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_007881.4

ORF Insert Sequence:

ATGAAGACAC GACAGAATAA AGACTCGATG TCAATGAGGA GTGGACGGAA GAAAGAGGCC 
CCCGGGCCCC GGGAAGAGCT GAGATCAAGG GGCCGGGCCT CCCCTGGAGG GGTCAGCACA 
TCCAGCAGTG ATGGCAAAGC TGAGAAGTCC AGGCAGACAG CCAAGAAGGC CCGGATAGAG 
GAGCCCTCTG CCCCAAAGGC CAGCAAGCAG GGCCGGAGCG AGGAGATCTC AGAGAGTGAG 
AGCGAGGAGA CCAGTGCGCC CAAAAAGACC AAAACCGAGC AGGAGCTCCC TCGCCCGCAG 
TCTCCCTCGG ATCTGGACAG CTTGGATGGG CGCAGCATTA ACGATGACGG CAGCAGCGAC 
CCTAGAGATA TAGACCAGGA CAACCGAAGC ACATCCCCCA GCATCTACAG CCCGGGCAGC 
GTGGAAAATG ACTCGGACTC ATCCTCTGGC CTGTCCCAGG GCCCCGCCCG CCCCTACCAC 
CCACCTCCAC TCTTCCCTCC TTCCCCTCCA CCACCAGACA GCACTCCCCG ACAGCCAGAG 
TCTGGCTTTG AACCTCATCC TTCTGTGCCG CCTACTGGAT ATCATGCTCC GATGGAGCCC 
CCCACATCGA GATTATTCCA GGGCCCACCA CCTGGAGCTC CTCCCACACA CCCACAGCTC 
TACCCTGGGA ATGCTAGTGG AGGTGTTTTA TCTGGACCCC CCATGGGTCC CAAAGGGGGA 
GCCGCTGCCT CCTCAGTGGG TGCCCCTAGC GGAGGCAAGC AACACCCCCC ACCCACTACC 
CCAATTCCAA TATCAAGTTC TGGGGCCAGT GGTGCTCCTC CAGCAAAGCC ACCCAGTGCT 
CCAGTGGGTG GTGGGAGCTT ACCTTCTGCA CCACCACCAG CTTCTTTCCC CCATGTGACA 
CCAAACCTGC CTCCTCCACC TGCCCTGAGA CCCCTCAACA ATGCCTCAGC CTCTCCTCCT 
GGCATGGGGG CTCAGCCAAT CCCTGGGCAT CTGCCCTCTC CCCATGCCAT GGGGCAGGGC 
ATGAGTGGAC TTCCTCCTGG CCCAGAGAAG GGTCCAACCC TGGCCCCTTC TCCCCACCCT 
TTGCCCCCAG CTTCTTCCTC TGCCCCTGGG CCTCCAATGC GATATCCATA TTCATCCTCC 
AGTAGCTCTG CCGCAGCCTC TTCTAGTTCC TCCTCCTCCT CTGCCTCCCA GTACCCTGCT 
TCCCAGGCCC TGCCCAGTTA TCCTCATTCC TTCCCCCCAC CAACTAGTAT GTCTGTCTCT 
AATCAGCCAC CCAAGTACAC CCAGCCTTCT CTCCCATCCC AAGCTGTGTG GAGCCAGGGT 
CCACCTCCTC CTCCTCCCTA TGGCCGCCTC TTGGCCAACA ACAACACCCA TCCAGGCCCT 
TTCCCTCCTA CTGGGGGTCA ATCTACAGCC CACCCAGCAG CCCCTACACA TCACCATCAC 
CAGCAGCAGC CACAGCAACA ACATCATCAT GGAAACTCTG GGCCCCCTCC ACCCGGAGCG 
TATCCTCACC CTCTAGAGAG CAGTAACTCC CATCATGCAC ACCCTTACAA CATGTCACCC 
TCCCTGGGGT CTTTAAGGCC CTACCCCCCA GGGCCAGCAC ACCTGCCTCC ACCTCATGGC 
CAGGTGTCCT ATAACCAAGC AGGTCCCAAT GGTCCCCCAG TTTCTTCTTC CAACTCTTCC 
GGGTCTTCCT CTCAAGCCTC CTATTCATGT TCACACCCCT CTTCATCCCA GGGCCCCCAA 
GGAGCATCCT ACCCCTTCCC ACCAGTCCCT CCAGTCACCA CCTCCTCAGC TACCCTTTCC 
ACTGTCATCG CCACCGTGGC TTCCTCGCCA GCAGGCTACA AAACAGCTTC GCCACCTGGG 
CCCCCTCAGT ACAGCAAGAG AGCCCCATCC CCAGGGTCCT ACAAGACAGC CACCCCGCCT 
GGATACAAAC CGGGGTCACC ACCCTCCTTC AGAACAGGGA CCCCACCCGG CTATCGAGGC 
ACCTCTCCGC CAGCAGGCCC AGGGACCTTC AAACCAGGTT CACCGACCGT GGGGCCGGGG 
CCCCTGCCAC CCGCGGGGCC TTCAAGTTTG TCATCTCTGC CTCCGCCACC TGCGGCCCCG 
ACTACAGGGC CGCCCCTGAC CGCCACGCAG ATCAAACAGG AGCCGGCGGA AGAGTATGAA 
CCTCCCGAGA GTCCGGTGCC TCCGGCCCGC AGCCCCTCGC CCCCTCCCAA GGTGGTGGAC 
GTGCCCAGCC ATGCCAGCCA GTCAGCCAGG TTCAATAAGC ACTTGGACCG CGGCTTCAAC 
TCGTGCGCGC GCAGCGACCT GTACTTCGTG CCGCTGGAGG GCTCCAAGCT GGCCAAGAAG 
CGCGCGGACC TGGTGGAGAA AGTGCGGCGC GAGGCCGAGC AGCGCGCGCG CGAGGAGAAA 
GAGCGCGAGC GCGAGCGGGA ACGCGAAAAG GAGCGCGAGC GCGAGAAAGA GCGCGAGCTG 
GAGCGCAGTG TGAAACTGGC CCAGGAGGGC CGTGCTCCAG TGGAGTGCCC ATCTCTGGGT 
CCAGTGCCCC ATCGGCCTCC CTTTGAGCCT GGCAGCGCTG TGGCTACAGT GCCCCCTTAC 
CTGGGTCCTG ATACTCCGGC CTTGCGCACT CTCAGTGAAT ACGCCCGACC TCATGTCATG 
TCTCCTGGCA ATCGCAACCA CCCATTCTAT GTGCCCTTGG GGGCAGTGGA CCCGGGGCTT 
CTGGGTTACA ATGTCCCAGC CCTGTACAGC AGCGACCCAG CTGCCCGAGA ACGGGAGCGG 
GAAGCCCGTG AACGTGACCT CCGTGACCGG CTCAAGCCTG GCTTTGAGGT GAAACCTAGT 
GAGCTGGAAC CCCTACATGG GGTTCCCGGG CCAGGCCTGG ATCCCTTCCC CCGACACGGG 
GGCCTGGCTC TACAGCCCGG GCCACCTGGC CTGCATCCTT TCCCTTTTCA TCCGAGCCTG 
GGGCCCCTGG AACGAGAACG GCTAGCGCTG GCAGCTGGGC CAGCCTTGCG TCCTGACATG 
TCTTATGCTG AGCGGTTGGC AGCTGAAAGG CAGCATGCAG AAAGGGTGGC AGCCCTGGGC 
AATGATCCAC TAGCCCGGCT GCAGATGCTC AACGTGACTC CCCATCACCA CCAGCACTCC 
CACATCCACT CTCACCTTCA CCTGCACCAG CAGGATGCTA TCCACGCAGC CTCTGCCTCG 
GTGCACCCTC TCATTGACCC CCTGGCCTCA GGGTCTCACC TTACCCGGAT CCCCTACCCA 
GCTGGGACCC TCCCCAACCC CCTTCTTCCT CACCCTCTGC ACGAGAACGA AGTTCTTCGT 
CACCAGCTTT TTGCTGCCCC TTACCGGGAC CTGCCGGCCT CCCTTTCTGC TCCAATGTCA 
GCGGCTCATC AGCTGCAGGC CATGCACGCG CAGTCAGCTG AGCTGCAGCG CTTGGCGCTG 
GAACAGCAGC AGTGGCTACA TGCTCATCAC CCATTGCACA GCGTGCCACT ACCTGCCCAG 
GAAGACTACT ACAGTCACCT GAAGAAGGAG AGTGACAAGC CGCTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Post-transcriptional regulation of mouse neurogenesis by Pumilio proteins.
Genes & development(2017 Aug)
Zhang M,Chen D,Xia J,Han W,Cui X,Neuenkirchen N,Hermes G,Sestan N,Lin H

High-throughput discovery of novel developmental phenotypes.
Nature537(7621)508-514(2016 09)
Dickinson ME,Flenniken AM,Ji X,Teboul L,Wong MD,White JK,Meehan TF,Weninger WJ,Westerberg H,Adissu H,Baker CN,Bower L,Brown JM,Caddle LB,Chiani F,Clary D,Cleak J,Daly MJ,Denegre JM,Doe B,Dolan ME,Edie SM,Fuchs H,Gailus-Durner V,Galli A,Gambadoro A,Gallegos J,Guo S,Horner NR,Hsu CW,Johnson SJ,Kalaga S,Keith LC,Lanoue L,Lawson TN,Lek M,Mark M,Marschall S,Mason J,McElwee ML,Newbigging S,Nutter LM,Peterson KA,Ramirez-Solis R,Rowland DJ,Ryder E,Samocha KE,Seavitt JR,Selloum M,Szoke-Kovacs Z,Tamura M,Trainor AG,Tudose I,Wakana S,Warren J,Wendling O,West DB,Wong L,Yoshiki A,,,,,,,,,MacArthur DG,Tocchini-Valentini GP,Gao X,Flicek P,Bradley A,Skarnes WC,Justice MJ,Parkinson HE,Moore M,Wells S,Braun RE,Svenson KL,de Angelis MH,Herault Y,Mohun T,Mallon AM,Henkelman RM,Brown SD,Adams DJ,Lloyd KC,McKerlie C,Beaudet AL,Bu?an M,Murray SA

Functional interactions between Fat family cadherins in tissue morphogenesis and planar polarity.
Development (Cambridge, England)139(10)1806-20(2012 May)
Saburi S,Hester I,Goodrich L,McNeill H

C-terminal deletion of the atrophin-1 protein results in growth retardation but not neurodegeneration in mice.
Developmental dynamics : an official publication of the American Association of Anatomists238(10)2471-8(2009 Oct)
Yu J,Ying M,Zhuang Y,Xu T,Han M,Wu X,Xu R

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Atn1 cDNA ORF clone, Mus musculus(house mouse)

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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