Ctsb cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Ctsb
Entrez Gene ID	13030
Full Name	cathepsin B
Synonyms	CB
General protein information	Preferred Names cathepsin B Names cathepsin B cathepsin B1
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 14 Map Location: 14 D1\|14 33.24 cM
Summary	This gene encodes a member of the peptidase C1 family and preproprotein that is proteolytically processed to generate multiple protein products. These products include the cathepsin B light and heavy chains, which can dimerize to generate the double chain form of the enzyme. This enzyme is a lysosomal cysteine protease with both endopeptidase and exopeptidase activity that may play a role in protein turnover. Homozygous knockout mice for this gene exhibit reduced pancreatic damage following induced pancreatitis and reduced hepatocyte apoptosis in a model of liver injury. Pseudogenes of this gene have been identified in the genome. [provided by RefSeq, Aug 2015].

mRNA and Protein(s)

mRNA	Protein	Name
NM_007798.3	NP_031824.1	cathepsin B preproprotein
XM_017315833.1	XP_017171322.1	cathepsin B isoform X1

Pathways from BioSystems

Homologies

Bos taurus (cattle)	CTSB	NP_776456.1
Gallus gallus (chicken)	CTSB	XP_004935955.1
Danio rerio (zebrafish)	ctsba	NP_998501.1
Homo sapiens (human)	CTSB	NP_680090.1
Canis lupus familiaris (dog)	CTSB	XP_543203.3
Rattus norvegicus (Norway rat)	Ctsb	NP_072119.2
Arabidopsis thaliana (thale cress)	AT1G02300	NP_563647.1
Oryza sativa (rice)	Os05g0310500	NP_001174338.1
Pan troglodytes (chimpanzee)	CTSB	XP_003317965.1
Xenopus tropicalis (tropical clawed frog)	ctsb	NP_989225.1
Drosophila melanogaster (fruit fly)	CtsB1	NP_572920.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP004533	XP_313835.5
Arabidopsis thaliana (thale cress)	AT1G02305	NP_563648.1
	CTSB	XP_002805301.1
Mus musculus (house mouse)	Ctsb	NP_031824.1
Arabidopsis thaliana (thale cress)	AT4G01610	NP_567215.1

Gene Ontology
Bibliography

Related articles in PubMed

Cathepsin D regulates cathepsin B activation and disease severity predominantly in inflammatory cells during experimental pancreatitis.
Aghdassi AA, John DS, Sendler M, Weiss FU, Reinheckel T, Mayerle J, Lerch MM
The Journal of biological chemistry293(3)1018-1029(2018 Jan)

Cathepsin B Regulates Hepatic Lipid Metabolism by Cleaving Liver Fatty Acid Binding Protein.
Thibeaux S, Siddiqi S, Zhelyabovska O, Moinuddin F, Masternak MM, Siddiqi SA
The Journal of biological chemistry(2017 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Our results suggest that the Mucopolysaccharidosis type I mice have progressive heart failure and valve disease, which may be caused by cathepsin B overexpression.
Title: Progressive heart disease in mucopolysaccharidosis type I mice may be mediated by increased cathepsin B activity.

Using quenched activity-based probes, we identified mouse cathepsin B (CtsB), which is expressed in the lower regions of the hair follicle, as an additional target of mouse Cst6
Title: Cathepsin B as a potential cystatin M/E target in the mouse hair follicle.

After phagocytosis, particles can disrupt lysosomes, and inhibitor studies have suggested that the resulting release of a lysosomal protease-cathepsin B-into the cytosol somehow activates NLRP3.
Title: Frontline Science: Multiple cathepsins promote inflammasome-independent, particle-induced cell death during NLRP3-dependent IL-1? activation.

ctsB enhanced neutrophil migration though a putative effect on pseudopod retraction rates.
Title: Fluid shear-induced cathepsin B release in the control of Mac1-dependent neutrophil adhesion.

Exposure of J774A.1 cells to HOCl or HOSCN resulted in a significant decrease in the activity of the Cys-dependent cathepsins B and L, but not the Asp-dependent cathepsin D.
Title: Role of Myeloperoxidase Oxidants in the Modulation of Cellular Lysosomal Enzyme Function: A Contributing Factor to Macrophage Dysfunction in Atherosclerosis?

The following Ctsb gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Ctsb cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu22736	NM_007798.3 Latest version!	Mus musculus cathepsin B (Ctsb), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$188.30 $269.00
OMu78659	XM_017315833.1 Latest version!	Mus musculus cathepsin B (Ctsb), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$139.30 $199.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Ctsb ( NM_007798.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_031824.1 Mus musculus cathepsin B (Ctsb), mRNA.

Price & Availability↑

CloneID	OMu22736
Clone ID Related Accession (Same CDS sequence)	NM_007798.3
Accession Version	NM_007798.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1020bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-27
Organism	Mus musculus(house mouse)
Product	cathepsin B preproprotein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK083393.1, AK151790.1, AC090654.38 and BI733634.1. On Nov 24, 2011 this sequence version replaced NM_007798.2. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK083393.1, AK151790.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTGGTGGT CCTTGATCCT TCTTTCTTGC CTGCTGGCAC TGACCAGTGC CCATGACAAG 
CCTTCCTTCC ACCCGCTGTC GGATGACCTG ATTAACTATA TCAACAAACA GAATACAACA 
TGGCAGGCTG GACGCAACTT CTACAATGTT GACATAAGCT ATCTGAAGAA GCTGTGTGGC 
ACTGTCCTGG GTGGACCCAA ACTGCCAGGA AGGGTTGCGT TCGGTGAGGA CATAGATCTA 
CCTGAAACCT TTGATGCACG GGAACAATGG TCCAACTGCC CGACCATTGG ACAGATTAGA 
GACCAGGGCT CCTGCGGCTC TTGTTGGGCA TTTGGGGCAG TGGAAGCCAT TTCTGACCGA 
ACCTGCATTC ACACCAATGG CCGAGTCAAC GTGGAGGTGT CTGCTGAAGA CCTGCTTACT 
TGCTGTGGTA TCCAGTGTGG GGACGGCTGT AATGGTGGCT ATCCCTCTGG AGCATGGAGC 
TTCTGGACAA AAAAAGGCCT GGTTTCAGGT GGAGTCTACA ATTCTCATGT AGGCTGCTTA 
CCATACACCA TCCCTCCCTG CGAGCACCAT GTCAATGGCT CCCGTCCCCC ATGCACTGGA 
GAAGGAGATA CTCCCAGGTG CAACAAGAGC TGTGAAGCTG GCTACTCCCC ATCCTACAAA 
GAGGATAAGC ACTTTGGGTA CACTTCCTAC AGCGTGTCTA ACAGTGTGAA GGAGATCATG 
GCAGAAATCT ACAAAAATGG CCCAGTGGAG GGTGCCTTCA CTGTGTTTTC TGACTTCTTG 
ACTTACAAAT CAGGAGTATA CAAGCATGAA GCCGGTGATA TGATGGGTGG CCACGCCATC 
CGCATCCTGG GCTGGGGAGT AGAGAATGGA GTTCCCTACT GGCTGGCAGC CAACTCTTGG 
AACCTTGACT GGGGTGATAA TGGCTTCTTT AAAATCCTCA GAGGAGAGAA CCACTGTGGC 
ATTGAATCAG AAATTGTGGC TGGAATCCCA CGCACTGACC AGTACTGGGG AAGATTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_031824.1
CDS	130..1149
Translation	MWWSLILLSCLLALTSAHDKPSFHPLSDDLINYINKQNTTWQAGRNFYNVDISYLKKLCGTVLGGPKLPGRVAFGEDIDLPETFDAREQWSNCPTIGQIRDQGSCGSCWAFGAVEAISDRTCIHTNGRVNVEVSAEDLLTCCGIQCGDGCNGGYPSGAWSFWTKKGLVSGGVYNSHVGCLPYTIPPCEHHVNGSRPPCTGEGDTPRCNKSCEAGYSPSYKEDKHFGYTSYSVSNSVKEIMAEIYKNGPVEGAFTVFSDFLTYKSGVYKHEAGDMMGGHAIRILGWGVENGVPYWLAANSWNLDWGDNGFFKILRGENHCGIESEIVAGIPRTDQYWGRF

Target ORF information:

RefSeq Version	NM_007798.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus cathepsin B (Ctsb), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_007798.3

ORF Insert Sequence:

ATGTGGTGGT CCTTGATCCT TCTTTCTTGC CTGCTGGCAC TGACCAGTGC CCATGACAAG 
CCTTCCTTCC ACCCGCTGTC GGATGACCTG ATTAACTATA TCAACAAACA GAATACAACA 
TGGCAGGCTG GACGCAACTT CTACAATGTT GACATAAGCT ATCTGAAGAA GCTGTGTGGC 
ACTGTCCTGG GTGGACCCAA ACTGCCAGGA AGGGTTGCGT TCGGTGAGGA CATAGATCTA 
CCTGAAACCT TTGATGCACG GGAACAATGG TCCAACTGCC CGACCATTGG ACAGATTAGA 
GACCAGGGCT CCTGCGGCTC TTGTTGGGCA TTTGGGGCAG TGGAAGCCAT TTCTGACCGA 
ACCTGCATTC ACACCAATGG CCGAGTCAAC GTGGAGGTGT CTGCTGAAGA CCTGCTTACT 
TGCTGTGGTA TCCAGTGTGG GGACGGCTGT AATGGTGGCT ATCCCTCTGG AGCATGGAGC 
TTCTGGACAA AAAAAGGCCT GGTTTCAGGT GGAGTCTACA ATTCTCATGT AGGCTGCTTA 
CCATACACCA TCCCTCCCTG CGAGCACCAT GTCAATGGCT CCCGTCCCCC ATGCACTGGA 
GAAGGAGATA CTCCCAGGTG CAACAAGAGC TGTGAAGCTG GCTACTCCCC ATCCTACAAA 
GAGGATAAGC ACTTTGGGTA CACTTCCTAC AGCGTGTCTA ACAGTGTGAA GGAGATCATG 
GCAGAAATCT ACAAAAATGG CCCAGTGGAG GGTGCCTTCA CTGTGTTTTC TGACTTCTTG 
ACTTACAAAT CAGGAGTATA CAAGCATGAA GCCGGTGATA TGATGGGTGG CCACGCCATC 
CGCATCCTGG GCTGGGGAGT AGAGAATGGA GTTCCCTACT GGCTGGCAGC CAACTCTTGG 
AACCTTGACT GGGGTGATAA TGGCTTCTTT AAAATCCTCA GAGGAGAGAA CCACTGTGGC 
ATTGAATCAG AAATTGTGGC TGGAATCCCA CGCACTGACC AGTACTGGGG AAGATTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ctsb ( XM_017315833.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017171322.1 Mus musculus cathepsin B (Ctsb), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu78659
Clone ID Related Accession (Same CDS sequence)	XM_017315833.1
Accession Version	XM_017315833.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 648bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	cathepsin B isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000080.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCACGGGA ACAATGGTCC AACTGCCCGA CCATTGGACA GATTAGAGAC CAGGGCTCCT 
GCGGCTCTTG TTGGCTGTAA TGGTGGCTAT CCCTCTGGAG CATGGAGCTT CTGGACAAAA 
AAAGGCCTGG TTTCAGGTGG AGTCTACAAT TCTCATGTAG GCTGCTTACC ATACACCATC 
CCTCCCTGCG AGCACCATGT CAATGGCTCC CGTCCCCCAT GCACTGGAGA AGGAGATACT 
CCCAGGTGCA ACAAGAGCTG TGAAGCTGGC TACTCCCCAT CCTACAAAGA GGATAAGCAC 
TTTGGGTACA CTTCCTACAG CGTGTCTAAC AGTGTGAAGG AGATCATGGC AGAAATCTAC 
AAAAATGGCC CAGTGGAGGG TGCCTTCACT GTGTTTTCTG ACTTCTTGAC TTACAAATCA 
GGAGTATACA AGCATGAAGC CGGTGATATG ATGGGTGGCC ACGCCATCCG CATCCTGGGC 
TGGGGAGTAG AGAATGGAGT TCCCTACTGG CTGGCAGCCA ACTCTTGGAA CCTTGACTGG 
GGTGATAATG GCTTCTTTAA AATCCTCAGA GGAGAGAACC ACTGTGGCAT TGAATCAGAA 
ATTGTGGCTG GAATCCCACG CACTGACCAG TACTGGGGAA GATTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017171322.1
CDS	1..648
Translation	MHGNNGPTARPLDRLETRAPAALVGCNGGYPSGAWSFWTKKGLVSGGVYNSHVGCLPYTIPPCEHHVNGSRPPCTGEGDTPRCNKSCEAGYSPSYKEDKHFGYTSYSVSNSVKEIMAEIYKNGPVEGAFTVFSDFLTYKSGVYKHEAGDMMGGHAIRILGWGVENGVPYWLAANSWNLDWGDNGFFKILRGENHCGIESEIVAGIPRTDQYWGRF

Target ORF information:

RefSeq Version	XM_017315833.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus cathepsin B (Ctsb), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017315833.1

ORF Insert Sequence:

ATGCACGGGA ACAATGGTCC AACTGCCCGA CCATTGGACA GATTAGAGAC CAGGGCTCCT 
GCGGCTCTTG TTGGCTGTAA TGGTGGCTAT CCCTCTGGAG CATGGAGCTT CTGGACAAAA 
AAAGGCCTGG TTTCAGGTGG AGTCTACAAT TCTCATGTAG GCTGCTTACC ATACACCATC 
CCTCCCTGCG AGCACCATGT CAATGGCTCC CGTCCCCCAT GCACTGGAGA AGGAGATACT 
CCCAGGTGCA ACAAGAGCTG TGAAGCTGGC TACTCCCCAT CCTACAAAGA GGATAAGCAC 
TTTGGGTACA CTTCCTACAG CGTGTCTAAC AGTGTGAAGG AGATCATGGC AGAAATCTAC 
AAAAATGGCC CAGTGGAGGG TGCCTTCACT GTGTTTTCTG ACTTCTTGAC TTACAAATCA 
GGAGTATACA AGCATGAAGC CGGTGATATG ATGGGTGGCC ACGCCATCCG CATCCTGGGC 
TGGGGAGTAG AGAATGGAGT TCCCTACTGG CTGGCAGCCA ACTCTTGGAA CCTTGACTGG 
GGTGATAATG GCTTCTTTAA AATCCTCAGA GGAGAGAACC ACTGTGGCAT TGAATCAGAA 
ATTGTGGCTG GAATCCCACG CACTGACCAG TACTGGGGAA GATTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Cathepsin D regulates cathepsin B activation and disease severity predominantly in inflammatory cells during experimental pancreatitis.
The Journal of biological chemistry293(3)1018-1029(2018 Jan)
Aghdassi AA,John DS,Sendler M,Weiss FU,Reinheckel T,Mayerle J,Lerch MM

Cathepsin B Regulates Hepatic Lipid Metabolism by Cleaving Liver Fatty Acid Binding Protein.
The Journal of biological chemistry(2017 Dec)
Thibeaux S,Siddiqi S,Zhelyabovska O,Moinuddin F,Masternak MM,Siddiqi SA

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Ctsb cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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