Cys1 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Cys1
Entrez Gene ID	12879
Full Name	cystin 1
Synonyms	2900006B19Rik,AV218859,Ccap,ck,cpk
General protein information	Preferred Names cystin 1 Names cystin-1 cilia-associated protein congenital polycystic kidneys cystin cilia associated protein
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 12 Map Location: 12 A1.3\|12 8.49 cM

mRNA and Protein(s)

mRNA	Protein	Name
NM_001004455.2	NP_001004455.2	cystin-1
XM_006514952.4	XP_006515015.1	cystin-1 isoform X1
NM_138686.3	NP_619627.3	cystin-1
NM_001161807.1	NP_001155279.1	cystin-1
XM_006514951.3	XP_006515014.1	cystin-1 isoform X1
XM_006514951.4	XP_006515014.1	cystin-1 isoform X1
XM_006514952.3	XP_006515015.1	cystin-1 isoform X1

Pathways from BioSystems

Homologies

Homo sapiens (human)	CYS1	NP_001032237.1
Pan troglodytes (chimpanzee)	CYS1	XP_001155543.1
Rattus norvegicus (Norway rat)	Cys1	NP_001103067.1
Mus musculus (house mouse)	Cys1	NP_619627.3
	CYS1	XP_001084741.1

Gene Ontology
Bibliography

Related articles in PubMed

HNF1B controls epithelial organization and cell polarity during ureteric bud branching and collecting duct morphogenesis.
Desgrange A, Heliot C, Skovorodkin I, Akram SU, Heikkil? J, Ronkainen VP, Miinalainen I, Vainio SJ, Cereghini S
Development (Cambridge, England)144(24)4704-4719(2017 Dec)

Hnf1b controls pancreas morphogenesis and the generation of Ngn3+ endocrine progenitors.
De Vas MG, Kopp JL, Heliot C, Sander M, Cereghini S, Haumaitre C
Development (Cambridge, England)142(5)871-82(2015 Mar)

Proliferation-Independent Initiation of Biliary Cysts in Polycystic Liver Diseases.
Beaudry JB, Cordi S, Demarez C, Lepreux S, Pierreux CE, Lemaigre FP
PloS one10(6)e0132295(2015)

Genetic and Informatic Analyses Implicate Kif12 as a Candidate Gene within the Mpkd2 Locus That Modulates Renal Cystic Disease Severity in the Cys1cpk Mouse.
Mrug M, Zhou J, Yang C, Aronow BJ, Cui X, Schoeb TR, Siegal GP, Yoder BK, Guay-Woodford LM
PloS one10(8)e0135678(2015)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Kif12 is a candidate gene within the major polycystic kidney disease modifier 2 locus that modulates renal cystic disease severity in the Cys1cpk mouse.
Title: Genetic and Informatic Analyses Implicate Kif12 as a Candidate Gene within the Mpkd2 Locus That Modulates Renal Cystic Disease Severity in the Cys1cpk Mouse.

Myc overexpression in the cpk kidney results from the dysregulation of the cystin-necdin regulatory complex and c-Myc, in turn, contributes to cystogenesis in the cpk mouse.
Title: The ciliary protein cystin forms a regulatory complex with necdin to modulate Myc expression.

Analysis of deletion and chimeric constructs identified an AxEGG motif that is necessary to target and retain cystin in the cilium. Derangement of these localization motifs may lead to cystic kidney disease.
Title: Cystin localizes to primary cilia via membrane microdomains and a targeting motif.

The polycystic kidney disease proteins, polycystin-1, polycystin-2, polaris, and cystin, are co-localized in renal cilia.
Title: The polycystic kidney disease proteins, polycystin-1, polycystin-2, polaris, and cystin, are co-localized in renal cilia.

The following Cys1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Cys1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu13594	XM_006514952.4 Latest version!	Mus musculus cystin 1 (Cys1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$69.30 $99.00
OMu13594	XM_006514951.4 Latest version!	Mus musculus cystin 1 (Cys1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$69.30 $99.00
OMu13594	NM_001161807.1 Latest version!	Mus musculus cystin 1 (Cys1), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$69.30 $99.00
OMu13594	NM_138686.3 Latest version!	Mus musculus cystin 1 (Cys1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$69.30 $99.00
OMu13594	NM_001004455.2 Latest version!	Mus musculus cystin 1 (Cys1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$69.30 $99.00
View Old Accession Versions >>
OMu13594	XM_006514951.3	Mus musculus cystin 1 (Cys1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$69.30 $99.00
OMu13594	XM_006514952.3	Mus musculus cystin 1 (Cys1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$69.30 $99.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Cys1 ( XM_006514952.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006515015.1 Mus musculus cystin 1 (Cys1), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OMu13594
Clone ID Related Accession (Same CDS sequence)	NM_001004455.2 , XM_006514952.4 , NM_138686.3 , NM_001161807.1 , XM_006514951.3 , XM_006514951.4 , XM_006514952.3
Accession Version	XM_006514952.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 438bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	cystin-1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000078.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006514952.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGCAGCG GCAGCAGCCG GAGCGGCCGG ATCCCGAGGC GGCGACGCAG CCCTGACAGG 
CGTCAGACAG GCCCGGGAGA GACAGCCTCG GAGGGCGGCA CGGCTGACCA GGCCCGGACG 
GCCGCGGGGC AGGAGGAGAG CGGCCGCGAC CCCCGACCCG CGACGCCCTC CGGTGGCCGG 
GAGGAGACCC TACGCCTGCT GGATCAGTTG CTGGCCGAGT CGGAGGCCTG GGGGCCCCAG 
GAGCTGACCC CACGAGGCCC CGCCCGGCTC GCACCCGCGG TGTCCCCAGA GAAGAAGGTT 
AAAGGCAACC CTGAAGACAG CTGTGCCTCT GAAGCCCCGG GGAACAGCCC CAAGAGACCC 
GAGGGGCAGT CGGCCATCTC CTATGACTAC TCAGAAGAGG AGCTCATGGC GAGCATTGAG 
CGGGAGTACT GCCGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006515015.1
CDS	979..1416
Translation	MGSGSSRSGRIPRRRRSPDRRQTGPGETASEGGTADQARTAAGQEESGRDPRPATPSGGREETLRLLDQLLAESEAWGPQELTPRGPARLAPAVSPEKKVKGNPEDSCASEAPGNSPKRPEGQSAISYDYSEEELMASIEREYCR

Target ORF information:

RefSeq Version	XM_006514952.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus cystin 1 (Cys1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006514952.4

ORF Insert Sequence:

ATGGGCAGCG GCAGCAGCCG GAGCGGCCGG ATCCCGAGGC GGCGACGCAG CCCTGACAGG 
CGTCAGACAG GCCCGGGAGA GACAGCCTCG GAGGGCGGCA CGGCTGACCA GGCCCGGACG 
GCCGCGGGGC AGGAGGAGAG CGGCCGCGAC CCCCGACCCG CGACGCCCTC CGGTGGCCGG 
GAGGAGACCC TACGCCTGCT GGATCAGTTG CTGGCCGAGT CGGAGGCCTG GGGGCCCCAG 
GAGCTGACCC CACGAGGCCC CGCCCGGCTC GCACCCGCGG TGTCCCCAGA GAAGAAGGTT 
AAAGGCAACC CTGAAGACAG CTGTGCCTCT GAAGCCCCGG GGAACAGCCC CAAGAGACCC 
GAGGGGCAGT CGGCCATCTC CTATGACTAC TCAGAAGAGG AGCTCATGGC GAGCATTGAG 
CGGGAGTACT GCCGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cys1 ( XM_006514951.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006515014.1 Mus musculus cystin 1 (Cys1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OMu13594
Clone ID Related Accession (Same CDS sequence)	NM_001004455.2 , XM_006514952.4 , NM_138686.3 , NM_001161807.1 , XM_006514951.3 , XM_006514951.4 , XM_006514952.3
Accession Version	XM_006514951.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 438bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	cystin-1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000078.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006514951.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGCAGCG GCAGCAGCCG GAGCGGCCGG ATCCCGAGGC GGCGACGCAG CCCTGACAGG 
CGTCAGACAG GCCCGGGAGA GACAGCCTCG GAGGGCGGCA CGGCTGACCA GGCCCGGACG 
GCCGCGGGGC AGGAGGAGAG CGGCCGCGAC CCCCGACCCG CGACGCCCTC CGGTGGCCGG 
GAGGAGACCC TACGCCTGCT GGATCAGTTG CTGGCCGAGT CGGAGGCCTG GGGGCCCCAG 
GAGCTGACCC CACGAGGCCC CGCCCGGCTC GCACCCGCGG TGTCCCCAGA GAAGAAGGTT 
AAAGGCAACC CTGAAGACAG CTGTGCCTCT GAAGCCCCGG GGAACAGCCC CAAGAGACCC 
GAGGGGCAGT CGGCCATCTC CTATGACTAC TCAGAAGAGG AGCTCATGGC GAGCATTGAG 
CGGGAGTACT GCCGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006515014.1
CDS	242..679
Translation	MGSGSSRSGRIPRRRRSPDRRQTGPGETASEGGTADQARTAAGQEESGRDPRPATPSGGREETLRLLDQLLAESEAWGPQELTPRGPARLAPAVSPEKKVKGNPEDSCASEAPGNSPKRPEGQSAISYDYSEEELMASIEREYCR

Target ORF information:

RefSeq Version	XM_006514951.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus cystin 1 (Cys1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006514951.4

ORF Insert Sequence:

ATGGGCAGCG GCAGCAGCCG GAGCGGCCGG ATCCCGAGGC GGCGACGCAG CCCTGACAGG 
CGTCAGACAG GCCCGGGAGA GACAGCCTCG GAGGGCGGCA CGGCTGACCA GGCCCGGACG 
GCCGCGGGGC AGGAGGAGAG CGGCCGCGAC CCCCGACCCG CGACGCCCTC CGGTGGCCGG 
GAGGAGACCC TACGCCTGCT GGATCAGTTG CTGGCCGAGT CGGAGGCCTG GGGGCCCCAG 
GAGCTGACCC CACGAGGCCC CGCCCGGCTC GCACCCGCGG TGTCCCCAGA GAAGAAGGTT 
AAAGGCAACC CTGAAGACAG CTGTGCCTCT GAAGCCCCGG GGAACAGCCC CAAGAGACCC 
GAGGGGCAGT CGGCCATCTC CTATGACTAC TCAGAAGAGG AGCTCATGGC GAGCATTGAG 
CGGGAGTACT GCCGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cys1 ( NM_001004455.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001004455.2 Mus musculus cystin 1 (Cys1), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OMu13594
Clone ID Related Accession (Same CDS sequence)	NM_001004455.2 , XM_006514952.4 , NM_138686.3 , NM_001161807.1 , XM_006514951.3 , XM_006514951.4 , XM_006514952.3
Accession Version	NM_001004455.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 438bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-24
Organism	Mus musculus(house mouse)
Product	cystin-1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AF390548.1 and BF658482.1. On Aug 2, 2006 this sequence version replaced NM_001004455.1. Transcript Variant: This variant (2) uses an alternate splice pattern in the 3' UTR, and encodes the same protein, as compared to variant 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC139302.1, BE996554.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## NMD candidate :: translation inferred from conservation ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGGCAGCG GCAGCAGCCG GAGCGGCCGG ATCCCGAGGC GGCGACGCAG CCCTGACAGG 
CGTCAGACAG GCCCGGGAGA GACAGCCTCG GAGGGCGGCA CGGCTGACCA GGCCCGGACG 
GCCGCGGGGC AGGAGGAGAG CGGCCGCGAC CCCCGACCCG CGACGCCCTC CGGTGGCCGG 
GAGGAGACCC TACGCCTGCT GGATCAGTTG CTGGCCGAGT CGGAGGCCTG GGGGCCCCAG 
GAGCTGACCC CACGAGGCCC CGCCCGGCTC GCACCCGCGG TGTCCCCAGA GAAGAAGGTT 
AAAGGCAACC CTGAAGACAG CTGTGCCTCT GAAGCCCCGG GGAACAGCCC CAAGAGACCC 
GAGGGGCAGT CGGCCATCTC CTATGACTAC TCAGAAGAGG AGCTCATGGC GAGCATTGAG 
CGGGAGTACT GCCGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001004455.2
CDS	888..1325
Translation	MGSGSSRSGRIPRRRRSPDRRQTGPGETASEGGTADQARTAAGQEESGRDPRPATPSGGREETLRLLDQLLAESEAWGPQELTPRGPARLAPAVSPEKKVKGNPEDSCASEAPGNSPKRPEGQSAISYDYSEEELMASIEREYCR

Target ORF information:

RefSeq Version	NM_001004455.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus cystin 1 (Cys1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001004455.2

ORF Insert Sequence:

ATGGGCAGCG GCAGCAGCCG GAGCGGCCGG ATCCCGAGGC GGCGACGCAG CCCTGACAGG 
CGTCAGACAG GCCCGGGAGA GACAGCCTCG GAGGGCGGCA CGGCTGACCA GGCCCGGACG 
GCCGCGGGGC AGGAGGAGAG CGGCCGCGAC CCCCGACCCG CGACGCCCTC CGGTGGCCGG 
GAGGAGACCC TACGCCTGCT GGATCAGTTG CTGGCCGAGT CGGAGGCCTG GGGGCCCCAG 
GAGCTGACCC CACGAGGCCC CGCCCGGCTC GCACCCGCGG TGTCCCCAGA GAAGAAGGTT 
AAAGGCAACC CTGAAGACAG CTGTGCCTCT GAAGCCCCGG GGAACAGCCC CAAGAGACCC 
GAGGGGCAGT CGGCCATCTC CTATGACTAC TCAGAAGAGG AGCTCATGGC GAGCATTGAG 
CGGGAGTACT GCCGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cys1 ( NM_138686.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_619627.3 Mus musculus cystin 1 (Cys1), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OMu13594
Clone ID Related Accession (Same CDS sequence)	NM_001004455.2 , XM_006514952.4 , NM_138686.3 , NM_001161807.1 , XM_006514951.3 , XM_006514951.4 , XM_006514952.3
Accession Version	NM_138686.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 438bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-24
Organism	Mus musculus(house mouse)
Product	cystin-1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AF390548.1 and BF658482.1. On Aug 2, 2006 this sequence version replaced NM_138686.2. Transcript Variant: This variant (1) represents the longer transcript. Both variants 1 and 2 encode the same protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF390548.1, BY712458.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164137 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## NMD candidate :: translation inferred from conservation ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGGCAGCG GCAGCAGCCG GAGCGGCCGG ATCCCGAGGC GGCGACGCAG CCCTGACAGG 
CGTCAGACAG GCCCGGGAGA GACAGCCTCG GAGGGCGGCA CGGCTGACCA GGCCCGGACG 
GCCGCGGGGC AGGAGGAGAG CGGCCGCGAC CCCCGACCCG CGACGCCCTC CGGTGGCCGG 
GAGGAGACCC TACGCCTGCT GGATCAGTTG CTGGCCGAGT CGGAGGCCTG GGGGCCCCAG 
GAGCTGACCC CACGAGGCCC CGCCCGGCTC GCACCCGCGG TGTCCCCAGA GAAGAAGGTT 
AAAGGCAACC CTGAAGACAG CTGTGCCTCT GAAGCCCCGG GGAACAGCCC CAAGAGACCC 
GAGGGGCAGT CGGCCATCTC CTATGACTAC TCAGAAGAGG AGCTCATGGC GAGCATTGAG 
CGGGAGTACT GCCGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_619627.3
CDS	888..1325
Translation	MGSGSSRSGRIPRRRRSPDRRQTGPGETASEGGTADQARTAAGQEESGRDPRPATPSGGREETLRLLDQLLAESEAWGPQELTPRGPARLAPAVSPEKKVKGNPEDSCASEAPGNSPKRPEGQSAISYDYSEEELMASIEREYCR

Target ORF information:

RefSeq Version	NM_138686.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus cystin 1 (Cys1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_138686.3

ORF Insert Sequence:

ATGGGCAGCG GCAGCAGCCG GAGCGGCCGG ATCCCGAGGC GGCGACGCAG CCCTGACAGG 
CGTCAGACAG GCCCGGGAGA GACAGCCTCG GAGGGCGGCA CGGCTGACCA GGCCCGGACG 
GCCGCGGGGC AGGAGGAGAG CGGCCGCGAC CCCCGACCCG CGACGCCCTC CGGTGGCCGG 
GAGGAGACCC TACGCCTGCT GGATCAGTTG CTGGCCGAGT CGGAGGCCTG GGGGCCCCAG 
GAGCTGACCC CACGAGGCCC CGCCCGGCTC GCACCCGCGG TGTCCCCAGA GAAGAAGGTT 
AAAGGCAACC CTGAAGACAG CTGTGCCTCT GAAGCCCCGG GGAACAGCCC CAAGAGACCC 
GAGGGGCAGT CGGCCATCTC CTATGACTAC TCAGAAGAGG AGCTCATGGC GAGCATTGAG 
CGGGAGTACT GCCGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cys1 ( NM_001161807.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001155279.1 Mus musculus cystin 1 (Cys1), transcript variant 3, mRNA.

Price & Availability↑

CloneID	OMu13594
Clone ID Related Accession (Same CDS sequence)	NM_001004455.2 , XM_006514952.4 , NM_138686.3 , NM_001161807.1 , XM_006514951.3 , XM_006514951.4 , XM_006514952.3
Accession Version	NM_001161807.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 438bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-24
Organism	Mus musculus(house mouse)
Product	cystin-1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC140262.4. Transcript Variant: This variant (3) uses an alternate splice pattern in the 3' UTR, and encodes the same protein, as compared to variant 1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC139298.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164137 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## NMD candidate :: translation inferred from conservation ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGGCAGCG GCAGCAGCCG GAGCGGCCGG ATCCCGAGGC GGCGACGCAG CCCTGACAGG 
CGTCAGACAG GCCCGGGAGA GACAGCCTCG GAGGGCGGCA CGGCTGACCA GGCCCGGACG 
GCCGCGGGGC AGGAGGAGAG CGGCCGCGAC CCCCGACCCG CGACGCCCTC CGGTGGCCGG 
GAGGAGACCC TACGCCTGCT GGATCAGTTG CTGGCCGAGT CGGAGGCCTG GGGGCCCCAG 
GAGCTGACCC CACGAGGCCC CGCCCGGCTC GCACCCGCGG TGTCCCCAGA GAAGAAGGTT 
AAAGGCAACC CTGAAGACAG CTGTGCCTCT GAAGCCCCGG GGAACAGCCC CAAGAGACCC 
GAGGGGCAGT CGGCCATCTC CTATGACTAC TCAGAAGAGG AGCTCATGGC GAGCATTGAG 
CGGGAGTACT GCCGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001155279.1
CDS	888..1325
Translation	MGSGSSRSGRIPRRRRSPDRRQTGPGETASEGGTADQARTAAGQEESGRDPRPATPSGGREETLRLLDQLLAESEAWGPQELTPRGPARLAPAVSPEKKVKGNPEDSCASEAPGNSPKRPEGQSAISYDYSEEELMASIEREYCR

Target ORF information:

RefSeq Version	NM_001161807.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus cystin 1 (Cys1), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001161807.1

ORF Insert Sequence:

ATGGGCAGCG GCAGCAGCCG GAGCGGCCGG ATCCCGAGGC GGCGACGCAG CCCTGACAGG 
CGTCAGACAG GCCCGGGAGA GACAGCCTCG GAGGGCGGCA CGGCTGACCA GGCCCGGACG 
GCCGCGGGGC AGGAGGAGAG CGGCCGCGAC CCCCGACCCG CGACGCCCTC CGGTGGCCGG 
GAGGAGACCC TACGCCTGCT GGATCAGTTG CTGGCCGAGT CGGAGGCCTG GGGGCCCCAG 
GAGCTGACCC CACGAGGCCC CGCCCGGCTC GCACCCGCGG TGTCCCCAGA GAAGAAGGTT 
AAAGGCAACC CTGAAGACAG CTGTGCCTCT GAAGCCCCGG GGAACAGCCC CAAGAGACCC 
GAGGGGCAGT CGGCCATCTC CTATGACTAC TCAGAAGAGG AGCTCATGGC GAGCATTGAG 
CGGGAGTACT GCCGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cys1 ( XM_006514951.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006515014.1 Mus musculus cystin 1 (Cys1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu13594
Clone ID Related Accession (Same CDS sequence)	NM_001004455.2 , XM_006514952.4 , NM_138686.3 , NM_001161807.1 , XM_006514951.3 , XM_006514951.4 , XM_006514952.3
Accession Version	XM_006514951.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 438bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	cystin-1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000078.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006514951.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGCAGCG GCAGCAGCCG GAGCGGCCGG ATCCCGAGGC GGCGACGCAG CCCTGACAGG 
CGTCAGACAG GCCCGGGAGA GACAGCCTCG GAGGGCGGCA CGGCTGACCA GGCCCGGACG 
GCCGCGGGGC AGGAGGAGAG CGGCCGCGAC CCCCGACCCG CGACGCCCTC CGGTGGCCGG 
GAGGAGACCC TACGCCTGCT GGATCAGTTG CTGGCCGAGT CGGAGGCCTG GGGGCCCCAG 
GAGCTGACCC CACGAGGCCC CGCCCGGCTC GCACCCGCGG TGTCCCCAGA GAAGAAGGTT 
AAAGGCAACC CTGAAGACAG CTGTGCCTCT GAAGCCCCGG GGAACAGCCC CAAGAGACCC 
GAGGGGCAGT CGGCCATCTC CTATGACTAC TCAGAAGAGG AGCTCATGGC GAGCATTGAG 
CGGGAGTACT GCCGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006515014.1
CDS	912..1349
Translation	MGSGSSRSGRIPRRRRSPDRRQTGPGETASEGGTADQARTAAGQEESGRDPRPATPSGGREETLRLLDQLLAESEAWGPQELTPRGPARLAPAVSPEKKVKGNPEDSCASEAPGNSPKRPEGQSAISYDYSEEELMASIEREYCR

Target ORF information:

RefSeq Version	XM_006514951.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus cystin 1 (Cys1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006514951.3

ORF Insert Sequence:

ATGGGCAGCG GCAGCAGCCG GAGCGGCCGG ATCCCGAGGC GGCGACGCAG CCCTGACAGG 
CGTCAGACAG GCCCGGGAGA GACAGCCTCG GAGGGCGGCA CGGCTGACCA GGCCCGGACG 
GCCGCGGGGC AGGAGGAGAG CGGCCGCGAC CCCCGACCCG CGACGCCCTC CGGTGGCCGG 
GAGGAGACCC TACGCCTGCT GGATCAGTTG CTGGCCGAGT CGGAGGCCTG GGGGCCCCAG 
GAGCTGACCC CACGAGGCCC CGCCCGGCTC GCACCCGCGG TGTCCCCAGA GAAGAAGGTT 
AAAGGCAACC CTGAAGACAG CTGTGCCTCT GAAGCCCCGG GGAACAGCCC CAAGAGACCC 
GAGGGGCAGT CGGCCATCTC CTATGACTAC TCAGAAGAGG AGCTCATGGC GAGCATTGAG 
CGGGAGTACT GCCGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cys1 ( XM_006514952.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006515015.1 Mus musculus cystin 1 (Cys1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OMu13594
Clone ID Related Accession (Same CDS sequence)	NM_001004455.2 , XM_006514952.4 , NM_138686.3 , NM_001161807.1 , XM_006514951.3 , XM_006514951.4 , XM_006514952.3
Accession Version	XM_006514952.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 438bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	cystin-1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000078.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006514952.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGCAGCG GCAGCAGCCG GAGCGGCCGG ATCCCGAGGC GGCGACGCAG CCCTGACAGG 
CGTCAGACAG GCCCGGGAGA GACAGCCTCG GAGGGCGGCA CGGCTGACCA GGCCCGGACG 
GCCGCGGGGC AGGAGGAGAG CGGCCGCGAC CCCCGACCCG CGACGCCCTC CGGTGGCCGG 
GAGGAGACCC TACGCCTGCT GGATCAGTTG CTGGCCGAGT CGGAGGCCTG GGGGCCCCAG 
GAGCTGACCC CACGAGGCCC CGCCCGGCTC GCACCCGCGG TGTCCCCAGA GAAGAAGGTT 
AAAGGCAACC CTGAAGACAG CTGTGCCTCT GAAGCCCCGG GGAACAGCCC CAAGAGACCC 
GAGGGGCAGT CGGCCATCTC CTATGACTAC TCAGAAGAGG AGCTCATGGC GAGCATTGAG 
CGGGAGTACT GCCGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006515015.1
CDS	912..1349
Translation	MGSGSSRSGRIPRRRRSPDRRQTGPGETASEGGTADQARTAAGQEESGRDPRPATPSGGREETLRLLDQLLAESEAWGPQELTPRGPARLAPAVSPEKKVKGNPEDSCASEAPGNSPKRPEGQSAISYDYSEEELMASIEREYCR

Target ORF information:

RefSeq Version	XM_006514952.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus cystin 1 (Cys1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006514952.3

ORF Insert Sequence:

ATGGGCAGCG GCAGCAGCCG GAGCGGCCGG ATCCCGAGGC GGCGACGCAG CCCTGACAGG 
CGTCAGACAG GCCCGGGAGA GACAGCCTCG GAGGGCGGCA CGGCTGACCA GGCCCGGACG 
GCCGCGGGGC AGGAGGAGAG CGGCCGCGAC CCCCGACCCG CGACGCCCTC CGGTGGCCGG 
GAGGAGACCC TACGCCTGCT GGATCAGTTG CTGGCCGAGT CGGAGGCCTG GGGGCCCCAG 
GAGCTGACCC CACGAGGCCC CGCCCGGCTC GCACCCGCGG TGTCCCCAGA GAAGAAGGTT 
AAAGGCAACC CTGAAGACAG CTGTGCCTCT GAAGCCCCGG GGAACAGCCC CAAGAGACCC 
GAGGGGCAGT CGGCCATCTC CTATGACTAC TCAGAAGAGG AGCTCATGGC GAGCATTGAG 
CGGGAGTACT GCCGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

HNF1B controls epithelial organization and cell polarity during ureteric bud branching and collecting duct morphogenesis.
Development (Cambridge, England)144(24)4704-4719(2017 Dec)
Desgrange A,Heliot C,Skovorodkin I,Akram SU,Heikkil? J,Ronkainen VP,Miinalainen I,Vainio SJ,Cereghini S

Hnf1b controls pancreas morphogenesis and the generation of Ngn3+ endocrine progenitors.
Development (Cambridge, England)142(5)871-82(2015 Mar)
De Vas MG,Kopp JL,Heliot C,Sander M,Cereghini S,Haumaitre C

Proliferation-Independent Initiation of Biliary Cysts in Polycystic Liver Diseases.
PloS one10(6)e0132295(2015)
Beaudry JB,Cordi S,Demarez C,Lepreux S,Pierreux CE,Lemaigre FP

Genetic and Informatic Analyses Implicate Kif12 as a Candidate Gene within the Mpkd2 Locus That Modulates Renal Cystic Disease Severity in the Cys1cpk Mouse.
PloS one10(8)e0135678(2015)
Mrug M,Zhou J,Yang C,Aronow BJ,Cui X,Schoeb TR,Siegal GP,Yoder BK,Guay-Woodford LM

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Cys1 cDNA ORF clone, Mus musculus(house mouse)

Gene

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gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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