Copa cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Copa
Entrez Gene ID	12847
Full Name	coatomer protein complex subunit alpha
Synonyms	AU040324,xenin
General protein information	Preferred Names coatomer protein complex subunit alpha Names coatomer subunit alpha alpha-COP alpha-coat protein
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 1 Map Location: 1 H3\|1 79.54 cM

mRNA and Protein(s)

mRNA	Protein	Name
NM_009938.4	NP_034068.3	coatomer subunit alpha
XM_030245513.1	XP_030101373.1	coatomer subunit alpha isoform X1
XM_011238748.2	XP_011237050.1	coatomer subunit alpha isoform X1

Pathways from BioSystems

Homologies

Homo sapiens (human)	COPA	NP_001091868.1
Mus musculus (house mouse)	Copa	NP_034068.3
Caenorhabditis elegans (roundworm)	Y71F9AL.17	NP_491069.1
Saccharomyces cerevisiae (baker's yeast)	COP1	NP_010136.1
Kluyveromyces lactis	KLLA0F04884g	XP_455301.1
Danio rerio (zebrafish)	copa	NP_001001941.2
Xenopus tropicalis (tropical clawed frog)	copa	NP_001121466.1
Bos taurus (cattle)	COPA	NP_001099115.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP010251	XP_319442.1
Magnaporthe oryzae (rice blast fungus)	MGG_03511	XP_003716408.1
Arabidopsis thaliana (thale cress)	AT1G62020	NP_176393.1
Rattus norvegicus (Norway rat)	Copa	NP_001128012.1
Gallus gallus (chicken)	COPA	NP_001026576.1
Drosophila melanogaster (fruit fly)	alphaCop	NP_477395.1
Eremothecium gossypii	AGOS_ADR077C	NP_984173.1
Schizosaccharomyces pombe (fission yeast)	SPBPJ4664.04	NP_595279.1
Oryza sativa (rice)	Os09g0127800	NP_001062612.1
Oryza sativa (rice)	Os03g0711400	NP_001051056.1
Pan troglodytes (chimpanzee)	COPA	XP_001171563.1
	COPA	XP_002801923.1
Canis lupus familiaris (dog)	COPA	XP_005640952.1
Neurospora crassa	NCU10066	XP_957881.1
Arabidopsis thaliana (thale cress)	AT2G21390	NP_179734.1

Gene Ontology
Bibliography

Related articles in PubMed

Relevance of the COPI complex for Alzheimer's disease progression in vivo.
Bettayeb K, Hooli BV, Parrado AR, Randolph L, Varotsis D, Aryal S, Gresack J, Tanzi RE, Greengard P, Flajolet M
Proceedings of the National Academy of Sciences of the United States of America113(19)5418-23(2016 May)

The COPI vesicle complex binds and moves with survival motor neuron within axons.
Peter CJ, Evans M, Thayanithy V, Taniguchi-Ishigaki N, Bach I, Kolpak A, Bassell GJ, Rossoll W, Lorson CL, Bao ZZ, Androphy EJ
Human molecular genetics20(9)1701-11(2011 May)

Knockdown of COPA, identified by loss-of-function screen, induces apoptosis and suppresses tumor growth in mesothelioma mouse model.
Sudo H, Tsuji AB, Sugyo A, Kohda M, Sogawa C, Yoshida C, Harada YN, Hino O, Saga T
Genomics95(4)210-6(2010 Apr)

Phylogenetic relationships of the seven coat protein subunits of the coatomer complex, and comparative sequence analysis of murine xenin and proxenin.
Chow VT, Sakharkar MK, Lim DP, Yeo WM
Biochemical genetics39(5-6)201-11(2001 Jun)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Remarkably, an improvement of the memory impairments was also observed. Importantly, human genetic association studies of different AD cohorts led to the identification of 12 SNPs and 24 mutations located in COPI genes linked to an increased AD risk.
Title: Relevance of the COPI complex for Alzheimer's disease progression in vivo.

The novel function of Copa in inter-compartmental trafficking of RNA.
Title: COPI transport complexes bind to specific RNAs in neuronal cells.

SMN directly binds to COPI and moves with survival motor neuron within axons.
Title: The COPI vesicle complex binds and moves with survival motor neuron within axons.

In a a large-scale functional screening of mesothelioma cells, the COPA protein was highly expressed in some mesothelioma cell lines but not in a pleural mesothelial cell line.
Title: Knockdown of COPA, identified by loss-of-function screen, induces apoptosis and suppresses tumor growth in mesothelioma mouse model.

The following Copa gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Copa cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu67435	XM_011238748.2 Latest version!	Mus musculus coatomer protein complex subunit alpha (Copa), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00
OMu67435	XM_030245513.1 Latest version!	Mus musculus coatomer protein complex subunit alpha (Copa), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OMu18270	NM_009938.4 Latest version!	Mus musculus coatomer protein complex subunit alpha (Copa), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$643.30 $919.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Copa ( XM_011238748.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011237050.1 Mus musculus coatomer protein complex subunit alpha (Copa), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu67435
Clone ID Related Accession (Same CDS sequence)	XM_030245513.1 , XM_011238748.2
Accession Version	XM_011238748.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2658bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	coatomer subunit alpha isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_011238748.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCTGCAA AGTCTCTTGC AGATTACAGA AAGTTCTTGG TAGAGAGTAG CCCGATTTGC 
GGTTCTAAGT TTCCAGTATT CAACATGTCA TACAATCCAG CAGAAAACGC AGTGCTGCTC 
TGCACCAGAG CAAGCAATCT AGAGAACAGT ACCTACGACC TATACACCAT CCCCAAAGAC 
GCAGACTCCC AGAACCCTGA CGCCCCTGAA GGGAAACGAT CCTCAGGCCT GACAGCGGTT 
TGGGTTGCTC GGAATCGGTT TGCTGTCCTA GATCGCATGC ATTCACTTCT GATTAAAAAC 
CTGAAAAATG AGATCACCAA GAAAATCCAG GTGCCAAACT GTGATGAAAT CTTCTATGCT 
GGTACAGGCA ACCTCCTGCT TCGGGATGCA GATTCCATCA CACTCTTTGA CGTCCAGCAG 
AAGCGAACTC TGGCATCAGT GAAGATTTCC AAGGTGAAAT ATGTTATCTG GTCGGCAGAC 
ATGTCTCATG TAGCGTTACT GGCCAAGCAT GCCATTGTGA TCTGTAACCG CAAGCTGGAT 
GCTCTGTGTA ACATTCATGA GAACATTCGT GTCAAGAGTG GGGCCTGGGA TGAAAGTGGG 
GTGTTTATCT ATACCACAAG CAACCACATC AAATATGCTG TCACCACTGG GGACCATGGG 
ATTATCCGAA CTCTGGATTT ACCCATCTAT GTCACCAGAG TGAAGGGCAA TAATGTATAC 
TGCCTAGACA GAGAGTGTCG TCCTCGGGTG CTAACCATTG ATCCCACTGA GTTCAAGTTC 
AAACTGGCCC TGATCAACAG AAAGTATGAT GAGGTACTCC ACATGGTGAG GAACGCTAAG 
CTGGTAGGCC AGTCAATCAT CGCTTATCTC CAGAAGAAGG GCTATCCTGA AGTGGCGCTG 
CATTTTGTAA AGGATGAGAA AACTCGTTTC AGCCTGGCCC TGGAGTGTGG AAACATTGAG 
ATTGCTCTAG AGGCAGCCAA AGCCCTGGAC GACAAGAACT GCTGGGAGAA GCTAGGAGAA 
GTGGCCCTGC TACAGGGCAA TCACCAAATT GTAGAGATGT GCTATCAGCG CACCAAAAAC 
TTTGACAAAC TTTCCTTCCT GTACCTTATT ACTGGCAACC TGGAGAAACT TCGAAAGATG 
ATGAAGATCG CTGAGATCCG AAAGGACATG AGTGGCCATT ATCAGAATGC CCTATACCTA 
GGTGATGTGT CAGAGAGAGT ACGGATCCTG AAGAACTGTG GGCAGAAATC CCTAGCCTAT 
CTCTCAGCTG CTACCCATGG CTTAGATGAA GAAGCTGAGA GCCTGAAGGA AACCTTTGAC 
CCTGAGAAGG AGACAATCCC AGACATTGAT CCTAATGCCA AGCTGCTCCA GCCACCTGCA 
CCTATCATGC CATTGGATAC AAACTGGCCC TTACTGACCG TGTCCAAAGG GTTCTTTGAA 
GGCTCCATTG CAAGCAAGGG GAAGGGAGGA GCATTGGCTG CTGACATTGA CATCGACACT 
GTAGGCACTG AAGGCTGGGG AGAGGACGCA GAGCTGCAGC TGGATGAAGA TGGGTTTGTG 
GAGGCTCCAG AAGGTTTGGG AGAGGATGTT CTTGGAAAGG GACAAGAAGA AGGAGGTGGC 
TGGGATGTAG AAGAAGATTT GGAGCTCCCA CCTGAGCTGG ATGTACCCTC TGGGGTGTCT 
GGTAGTGCTG AAGATGGGTT CTTTGTGCCC CCAACCAAGG GAACAAGCCC AACGCAAATC 
TGGTGCAATA ACTCCCAGCT TCCAGTAGAT CACATCTTGG CAGGTTCTTT TGAAACAGCC 
ATGCGGCTTC TCCATGATCA GGTGGGAGTA ATCCAGTTTG GCCCCTATAA GCAACTGTTC 
CTACAGACAT ATGCCCGAGG CCGCACCACC TATCAAGCGC TGCCCTGCTT GCCCTCCATG 
TATAGTTACC CTAATCGCAA TTGGAAGGAT GCAGGGCTAA AGAATGGAGT ACCAGCTGTA 
GGCCTAAAGC TTAATGACCT GATCCAACGT TTGCAACTGT GTTATCAGCT CACCACAGTT 
GGCAAGTTTG AGGAAGCTGT GGAAAAATTC CGTTCCATCC TACTCAGTGT GCCTCTTCTT 
GTTGTGGACA ATAAACAGGA GATTGCAGAG GCCCAGCAGC TCATCACCAT TTGCCGTGAA 
TACATTGTGG GTTTGTGCAT GGAGATAGAA AGGAAGAAGC TACCTAAGGA GACCCTAGAC 
CAGCAGAAGC GCATCTGTGA GATGGCAGCC TACTTCACAC ACTCAAATCT GCAGCCTGTG 
CATATGATCT TGGTGCTGCG GACAGCCCTC AACCTTTTCT TCAAGCTCAA GAACTTCAAG 
ACAGCTGCCA CCTTTGCCCG ACGCCTGCTG GAACTTGGGC CCAAGCCTGA AGTAGCTCAG 
CAGACAAGAA AAATCTTATC TGCCTGTGAG AAAAACCCCA CAGATGCCTG CCAGCTCAAT 
TATGACATGC ACAACCCCTT TGACATTTGT GCTGCATCTT ACCGGCCCAT CTACCGTGGA 
AAACCAGTGG AGAAGTGTCC ACTCAGTGGG GCCTGCTATT CCCCTGAGTT CAAGGGGCAA 
ATCTGCAGGG TCACTACAGT GACAGAGATT GGCAAAGATG TGATTGGGCT GAGGATCAGT 
CCTCTTCAGT TTCGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011237050.1
CDS	485..3142
Translation	MPAKSLADYRKFLVESSPICGSKFPVFNMSYNPAENAVLLCTRASNLENSTYDLYTIPKDADSQNPDAPEGKRSSGLTAVWVARNRFAVLDRMHSLLIKNLKNEITKKIQVPNCDEIFYAGTGNLLLRDADSITLFDVQQKRTLASVKISKVKYVIWSADMSHVALLAKHAIVICNRKLDALCNIHENIRVKSGAWDESGVFIYTTSNHIKYAVTTGDHGIIRTLDLPIYVTRVKGNNVYCLDRECRPRVLTIDPTEFKFKLALINRKYDEVLHMVRNAKLVGQSIIAYLQKKGYPEVALHFVKDEKTRFSLALECGNIEIALEAAKALDDKNCWEKLGEVALLQGNHQIVEMCYQRTKNFDKLSFLYLITGNLEKLRKMMKIAEIRKDMSGHYQNALYLGDVSERVRILKNCGQKSLAYLSAATHGLDEEAESLKETFDPEKETIPDIDPNAKLLQPPAPIMPLDTNWPLLTVSKGFFEGSIASKGKGGALAADIDIDTVGTEGWGEDAELQLDEDGFVEAPEGLGEDVLGKGQEEGGGWDVEEDLELPPELDVPSGVSGSAEDGFFVPPTKGTSPTQIWCNNSQLPVDHILAGSFETAMRLLHDQVGVIQFGPYKQLFLQTYARGRTTYQALPCLPSMYSYPNRNWKDAGLKNGVPAVGLKLNDLIQRLQLCYQLTTVGKFEEAVEKFRSILLSVPLLVVDNKQEIAEAQQLITICREYIVGLCMEIERKKLPKETLDQQKRICEMAAYFTHSNLQPVHMILVLRTALNLFFKLKNFKTAATFARRLLELGPKPEVAQQTRKILSACEKNPTDACQLNYDMHNPFDICAASYRPIYRGKPVEKCPLSGACYSPEFKGQICRVTTVTEIGKDVIGLRISPLQFR

Target ORF information:

RefSeq Version	XM_011238748.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus coatomer protein complex subunit alpha (Copa), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011238748.2

ORF Insert Sequence:

ATGCCTGCAA AGTCTCTTGC AGATTACAGA AAGTTCTTGG TAGAGAGTAG CCCGATTTGC 
GGTTCTAAGT TTCCAGTATT CAACATGTCA TACAATCCAG CAGAAAACGC AGTGCTGCTC 
TGCACCAGAG CAAGCAATCT AGAGAACAGT ACCTACGACC TATACACCAT CCCCAAAGAC 
GCAGACTCCC AGAACCCTGA CGCCCCTGAA GGGAAACGAT CCTCAGGCCT GACAGCGGTT 
TGGGTTGCTC GGAATCGGTT TGCTGTCCTA GATCGCATGC ATTCACTTCT GATTAAAAAC 
CTGAAAAATG AGATCACCAA GAAAATCCAG GTGCCAAACT GTGATGAAAT CTTCTATGCT 
GGTACAGGCA ACCTCCTGCT TCGGGATGCA GATTCCATCA CACTCTTTGA CGTCCAGCAG 
AAGCGAACTC TGGCATCAGT GAAGATTTCC AAGGTGAAAT ATGTTATCTG GTCGGCAGAC 
ATGTCTCATG TAGCGTTACT GGCCAAGCAT GCCATTGTGA TCTGTAACCG CAAGCTGGAT 
GCTCTGTGTA ACATTCATGA GAACATTCGT GTCAAGAGTG GGGCCTGGGA TGAAAGTGGG 
GTGTTTATCT ATACCACAAG CAACCACATC AAATATGCTG TCACCACTGG GGACCATGGG 
ATTATCCGAA CTCTGGATTT ACCCATCTAT GTCACCAGAG TGAAGGGCAA TAATGTATAC 
TGCCTAGACA GAGAGTGTCG TCCTCGGGTG CTAACCATTG ATCCCACTGA GTTCAAGTTC 
AAACTGGCCC TGATCAACAG AAAGTATGAT GAGGTACTCC ACATGGTGAG GAACGCTAAG 
CTGGTAGGCC AGTCAATCAT CGCTTATCTC CAGAAGAAGG GCTATCCTGA AGTGGCGCTG 
CATTTTGTAA AGGATGAGAA AACTCGTTTC AGCCTGGCCC TGGAGTGTGG AAACATTGAG 
ATTGCTCTAG AGGCAGCCAA AGCCCTGGAC GACAAGAACT GCTGGGAGAA GCTAGGAGAA 
GTGGCCCTGC TACAGGGCAA TCACCAAATT GTAGAGATGT GCTATCAGCG CACCAAAAAC 
TTTGACAAAC TTTCCTTCCT GTACCTTATT ACTGGCAACC TGGAGAAACT TCGAAAGATG 
ATGAAGATCG CTGAGATCCG AAAGGACATG AGTGGCCATT ATCAGAATGC CCTATACCTA 
GGTGATGTGT CAGAGAGAGT ACGGATCCTG AAGAACTGTG GGCAGAAATC CCTAGCCTAT 
CTCTCAGCTG CTACCCATGG CTTAGATGAA GAAGCTGAGA GCCTGAAGGA AACCTTTGAC 
CCTGAGAAGG AGACAATCCC AGACATTGAT CCTAATGCCA AGCTGCTCCA GCCACCTGCA 
CCTATCATGC CATTGGATAC AAACTGGCCC TTACTGACCG TGTCCAAAGG GTTCTTTGAA 
GGCTCCATTG CAAGCAAGGG GAAGGGAGGA GCATTGGCTG CTGACATTGA CATCGACACT 
GTAGGCACTG AAGGCTGGGG AGAGGACGCA GAGCTGCAGC TGGATGAAGA TGGGTTTGTG 
GAGGCTCCAG AAGGTTTGGG AGAGGATGTT CTTGGAAAGG GACAAGAAGA AGGAGGTGGC 
TGGGATGTAG AAGAAGATTT GGAGCTCCCA CCTGAGCTGG ATGTACCCTC TGGGGTGTCT 
GGTAGTGCTG AAGATGGGTT CTTTGTGCCC CCAACCAAGG GAACAAGCCC AACGCAAATC 
TGGTGCAATA ACTCCCAGCT TCCAGTAGAT CACATCTTGG CAGGTTCTTT TGAAACAGCC 
ATGCGGCTTC TCCATGATCA GGTGGGAGTA ATCCAGTTTG GCCCCTATAA GCAACTGTTC 
CTACAGACAT ATGCCCGAGG CCGCACCACC TATCAAGCGC TGCCCTGCTT GCCCTCCATG 
TATAGTTACC CTAATCGCAA TTGGAAGGAT GCAGGGCTAA AGAATGGAGT ACCAGCTGTA 
GGCCTAAAGC TTAATGACCT GATCCAACGT TTGCAACTGT GTTATCAGCT CACCACAGTT 
GGCAAGTTTG AGGAAGCTGT GGAAAAATTC CGTTCCATCC TACTCAGTGT GCCTCTTCTT 
GTTGTGGACA ATAAACAGGA GATTGCAGAG GCCCAGCAGC TCATCACCAT TTGCCGTGAA 
TACATTGTGG GTTTGTGCAT GGAGATAGAA AGGAAGAAGC TACCTAAGGA GACCCTAGAC 
CAGCAGAAGC GCATCTGTGA GATGGCAGCC TACTTCACAC ACTCAAATCT GCAGCCTGTG 
CATATGATCT TGGTGCTGCG GACAGCCCTC AACCTTTTCT TCAAGCTCAA GAACTTCAAG 
ACAGCTGCCA CCTTTGCCCG ACGCCTGCTG GAACTTGGGC CCAAGCCTGA AGTAGCTCAG 
CAGACAAGAA AAATCTTATC TGCCTGTGAG AAAAACCCCA CAGATGCCTG CCAGCTCAAT 
TATGACATGC ACAACCCCTT TGACATTTGT GCTGCATCTT ACCGGCCCAT CTACCGTGGA 
AAACCAGTGG AGAAGTGTCC ACTCAGTGGG GCCTGCTATT CCCCTGAGTT CAAGGGGCAA 
ATCTGCAGGG TCACTACAGT GACAGAGATT GGCAAAGATG TGATTGGGCT GAGGATCAGT 
CCTCTTCAGT TTCGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Copa ( XM_030245513.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030101373.1 Mus musculus coatomer protein complex subunit alpha (Copa), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu67435
Clone ID Related Accession (Same CDS sequence)	XM_030245513.1 , XM_011238748.2
Accession Version	XM_030245513.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2658bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	coatomer subunit alpha isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCTGCAA AGTCTCTTGC AGATTACAGA AAGTTCTTGG TAGAGAGTAG CCCGATTTGC 
GGTTCTAAGT TTCCAGTATT CAACATGTCA TACAATCCAG CAGAAAACGC AGTGCTGCTC 
TGCACCAGAG CAAGCAATCT AGAGAACAGT ACCTACGACC TATACACCAT CCCCAAAGAC 
GCAGACTCCC AGAACCCTGA CGCCCCTGAA GGGAAACGAT CCTCAGGCCT GACAGCGGTT 
TGGGTTGCTC GGAATCGGTT TGCTGTCCTA GATCGCATGC ATTCACTTCT GATTAAAAAC 
CTGAAAAATG AGATCACCAA GAAAATCCAG GTGCCAAACT GTGATGAAAT CTTCTATGCT 
GGTACAGGCA ACCTCCTGCT TCGGGATGCA GATTCCATCA CACTCTTTGA CGTCCAGCAG 
AAGCGAACTC TGGCATCAGT GAAGATTTCC AAGGTGAAAT ATGTTATCTG GTCGGCAGAC 
ATGTCTCATG TAGCGTTACT GGCCAAGCAT GCCATTGTGA TCTGTAACCG CAAGCTGGAT 
GCTCTGTGTA ACATTCATGA GAACATTCGT GTCAAGAGTG GGGCCTGGGA TGAAAGTGGG 
GTGTTTATCT ATACCACAAG CAACCACATC AAATATGCTG TCACCACTGG GGACCATGGG 
ATTATCCGAA CTCTGGATTT ACCCATCTAT GTCACCAGAG TGAAGGGCAA TAATGTATAC 
TGCCTAGACA GAGAGTGTCG TCCTCGGGTG CTAACCATTG ATCCCACTGA GTTCAAGTTC 
AAACTGGCCC TGATCAACAG AAAGTATGAT GAGGTACTCC ACATGGTGAG GAACGCTAAG 
CTGGTAGGCC AGTCAATCAT CGCTTATCTC CAGAAGAAGG GCTATCCTGA AGTGGCGCTG 
CATTTTGTAA AGGATGAGAA AACTCGTTTC AGCCTGGCCC TGGAGTGTGG AAACATTGAG 
ATTGCTCTAG AGGCAGCCAA AGCCCTGGAC GACAAGAACT GCTGGGAGAA GCTAGGAGAA 
GTGGCCCTGC TACAGGGCAA TCACCAAATT GTAGAGATGT GCTATCAGCG CACCAAAAAC 
TTTGACAAAC TTTCCTTCCT GTACCTTATT ACTGGCAACC TGGAGAAACT TCGAAAGATG 
ATGAAGATCG CTGAGATCCG AAAGGACATG AGTGGCCATT ATCAGAATGC CCTATACCTA 
GGTGATGTGT CAGAGAGAGT ACGGATCCTG AAGAACTGTG GGCAGAAATC CCTAGCCTAT 
CTCTCAGCTG CTACCCATGG CTTAGATGAA GAAGCTGAGA GCCTGAAGGA AACCTTTGAC 
CCTGAGAAGG AGACAATCCC AGACATTGAT CCTAATGCCA AGCTGCTCCA GCCACCTGCA 
CCTATCATGC CATTGGATAC AAACTGGCCC TTACTGACCG TGTCCAAAGG GTTCTTTGAA 
GGCTCCATTG CAAGCAAGGG GAAGGGAGGA GCATTGGCTG CTGACATTGA CATCGACACT 
GTAGGCACTG AAGGCTGGGG AGAGGACGCA GAGCTGCAGC TGGATGAAGA TGGGTTTGTG 
GAGGCTCCAG AAGGTTTGGG AGAGGATGTT CTTGGAAAGG GACAAGAAGA AGGAGGTGGC 
TGGGATGTAG AAGAAGATTT GGAGCTCCCA CCTGAGCTGG ATGTACCCTC TGGGGTGTCT 
GGTAGTGCTG AAGATGGGTT CTTTGTGCCC CCAACCAAGG GAACAAGCCC AACGCAAATC 
TGGTGCAATA ACTCCCAGCT TCCAGTAGAT CACATCTTGG CAGGTTCTTT TGAAACAGCC 
ATGCGGCTTC TCCATGATCA GGTGGGAGTA ATCCAGTTTG GCCCCTATAA GCAACTGTTC 
CTACAGACAT ATGCCCGAGG CCGCACCACC TATCAAGCGC TGCCCTGCTT GCCCTCCATG 
TATAGTTACC CTAATCGCAA TTGGAAGGAT GCAGGGCTAA AGAATGGAGT ACCAGCTGTA 
GGCCTAAAGC TTAATGACCT GATCCAACGT TTGCAACTGT GTTATCAGCT CACCACAGTT 
GGCAAGTTTG AGGAAGCTGT GGAAAAATTC CGTTCCATCC TACTCAGTGT GCCTCTTCTT 
GTTGTGGACA ATAAACAGGA GATTGCAGAG GCCCAGCAGC TCATCACCAT TTGCCGTGAA 
TACATTGTGG GTTTGTGCAT GGAGATAGAA AGGAAGAAGC TACCTAAGGA GACCCTAGAC 
CAGCAGAAGC GCATCTGTGA GATGGCAGCC TACTTCACAC ACTCAAATCT GCAGCCTGTG 
CATATGATCT TGGTGCTGCG GACAGCCCTC AACCTTTTCT TCAAGCTCAA GAACTTCAAG 
ACAGCTGCCA CCTTTGCCCG ACGCCTGCTG GAACTTGGGC CCAAGCCTGA AGTAGCTCAG 
CAGACAAGAA AAATCTTATC TGCCTGTGAG AAAAACCCCA CAGATGCCTG CCAGCTCAAT 
TATGACATGC ACAACCCCTT TGACATTTGT GCTGCATCTT ACCGGCCCAT CTACCGTGGA 
AAACCAGTGG AGAAGTGTCC ACTCAGTGGG GCCTGCTATT CCCCTGAGTT CAAGGGGCAA 
ATCTGCAGGG TCACTACAGT GACAGAGATT GGCAAAGATG TGATTGGGCT GAGGATCAGT 
CCTCTTCAGT TTCGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030101373.1
CDS	1226..3883
Translation	MPAKSLADYRKFLVESSPICGSKFPVFNMSYNPAENAVLLCTRASNLENSTYDLYTIPKDADSQNPDAPEGKRSSGLTAVWVARNRFAVLDRMHSLLIKNLKNEITKKIQVPNCDEIFYAGTGNLLLRDADSITLFDVQQKRTLASVKISKVKYVIWSADMSHVALLAKHAIVICNRKLDALCNIHENIRVKSGAWDESGVFIYTTSNHIKYAVTTGDHGIIRTLDLPIYVTRVKGNNVYCLDRECRPRVLTIDPTEFKFKLALINRKYDEVLHMVRNAKLVGQSIIAYLQKKGYPEVALHFVKDEKTRFSLALECGNIEIALEAAKALDDKNCWEKLGEVALLQGNHQIVEMCYQRTKNFDKLSFLYLITGNLEKLRKMMKIAEIRKDMSGHYQNALYLGDVSERVRILKNCGQKSLAYLSAATHGLDEEAESLKETFDPEKETIPDIDPNAKLLQPPAPIMPLDTNWPLLTVSKGFFEGSIASKGKGGALAADIDIDTVGTEGWGEDAELQLDEDGFVEAPEGLGEDVLGKGQEEGGGWDVEEDLELPPELDVPSGVSGSAEDGFFVPPTKGTSPTQIWCNNSQLPVDHILAGSFETAMRLLHDQVGVIQFGPYKQLFLQTYARGRTTYQALPCLPSMYSYPNRNWKDAGLKNGVPAVGLKLNDLIQRLQLCYQLTTVGKFEEAVEKFRSILLSVPLLVVDNKQEIAEAQQLITICREYIVGLCMEIERKKLPKETLDQQKRICEMAAYFTHSNLQPVHMILVLRTALNLFFKLKNFKTAATFARRLLELGPKPEVAQQTRKILSACEKNPTDACQLNYDMHNPFDICAASYRPIYRGKPVEKCPLSGACYSPEFKGQICRVTTVTEIGKDVIGLRISPLQFR

Target ORF information:

RefSeq Version	XM_030245513.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus coatomer protein complex subunit alpha (Copa), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030245513.1

ORF Insert Sequence:

ATGCCTGCAA AGTCTCTTGC AGATTACAGA AAGTTCTTGG TAGAGAGTAG CCCGATTTGC 
GGTTCTAAGT TTCCAGTATT CAACATGTCA TACAATCCAG CAGAAAACGC AGTGCTGCTC 
TGCACCAGAG CAAGCAATCT AGAGAACAGT ACCTACGACC TATACACCAT CCCCAAAGAC 
GCAGACTCCC AGAACCCTGA CGCCCCTGAA GGGAAACGAT CCTCAGGCCT GACAGCGGTT 
TGGGTTGCTC GGAATCGGTT TGCTGTCCTA GATCGCATGC ATTCACTTCT GATTAAAAAC 
CTGAAAAATG AGATCACCAA GAAAATCCAG GTGCCAAACT GTGATGAAAT CTTCTATGCT 
GGTACAGGCA ACCTCCTGCT TCGGGATGCA GATTCCATCA CACTCTTTGA CGTCCAGCAG 
AAGCGAACTC TGGCATCAGT GAAGATTTCC AAGGTGAAAT ATGTTATCTG GTCGGCAGAC 
ATGTCTCATG TAGCGTTACT GGCCAAGCAT GCCATTGTGA TCTGTAACCG CAAGCTGGAT 
GCTCTGTGTA ACATTCATGA GAACATTCGT GTCAAGAGTG GGGCCTGGGA TGAAAGTGGG 
GTGTTTATCT ATACCACAAG CAACCACATC AAATATGCTG TCACCACTGG GGACCATGGG 
ATTATCCGAA CTCTGGATTT ACCCATCTAT GTCACCAGAG TGAAGGGCAA TAATGTATAC 
TGCCTAGACA GAGAGTGTCG TCCTCGGGTG CTAACCATTG ATCCCACTGA GTTCAAGTTC 
AAACTGGCCC TGATCAACAG AAAGTATGAT GAGGTACTCC ACATGGTGAG GAACGCTAAG 
CTGGTAGGCC AGTCAATCAT CGCTTATCTC CAGAAGAAGG GCTATCCTGA AGTGGCGCTG 
CATTTTGTAA AGGATGAGAA AACTCGTTTC AGCCTGGCCC TGGAGTGTGG AAACATTGAG 
ATTGCTCTAG AGGCAGCCAA AGCCCTGGAC GACAAGAACT GCTGGGAGAA GCTAGGAGAA 
GTGGCCCTGC TACAGGGCAA TCACCAAATT GTAGAGATGT GCTATCAGCG CACCAAAAAC 
TTTGACAAAC TTTCCTTCCT GTACCTTATT ACTGGCAACC TGGAGAAACT TCGAAAGATG 
ATGAAGATCG CTGAGATCCG AAAGGACATG AGTGGCCATT ATCAGAATGC CCTATACCTA 
GGTGATGTGT CAGAGAGAGT ACGGATCCTG AAGAACTGTG GGCAGAAATC CCTAGCCTAT 
CTCTCAGCTG CTACCCATGG CTTAGATGAA GAAGCTGAGA GCCTGAAGGA AACCTTTGAC 
CCTGAGAAGG AGACAATCCC AGACATTGAT CCTAATGCCA AGCTGCTCCA GCCACCTGCA 
CCTATCATGC CATTGGATAC AAACTGGCCC TTACTGACCG TGTCCAAAGG GTTCTTTGAA 
GGCTCCATTG CAAGCAAGGG GAAGGGAGGA GCATTGGCTG CTGACATTGA CATCGACACT 
GTAGGCACTG AAGGCTGGGG AGAGGACGCA GAGCTGCAGC TGGATGAAGA TGGGTTTGTG 
GAGGCTCCAG AAGGTTTGGG AGAGGATGTT CTTGGAAAGG GACAAGAAGA AGGAGGTGGC 
TGGGATGTAG AAGAAGATTT GGAGCTCCCA CCTGAGCTGG ATGTACCCTC TGGGGTGTCT 
GGTAGTGCTG AAGATGGGTT CTTTGTGCCC CCAACCAAGG GAACAAGCCC AACGCAAATC 
TGGTGCAATA ACTCCCAGCT TCCAGTAGAT CACATCTTGG CAGGTTCTTT TGAAACAGCC 
ATGCGGCTTC TCCATGATCA GGTGGGAGTA ATCCAGTTTG GCCCCTATAA GCAACTGTTC 
CTACAGACAT ATGCCCGAGG CCGCACCACC TATCAAGCGC TGCCCTGCTT GCCCTCCATG 
TATAGTTACC CTAATCGCAA TTGGAAGGAT GCAGGGCTAA AGAATGGAGT ACCAGCTGTA 
GGCCTAAAGC TTAATGACCT GATCCAACGT TTGCAACTGT GTTATCAGCT CACCACAGTT 
GGCAAGTTTG AGGAAGCTGT GGAAAAATTC CGTTCCATCC TACTCAGTGT GCCTCTTCTT 
GTTGTGGACA ATAAACAGGA GATTGCAGAG GCCCAGCAGC TCATCACCAT TTGCCGTGAA 
TACATTGTGG GTTTGTGCAT GGAGATAGAA AGGAAGAAGC TACCTAAGGA GACCCTAGAC 
CAGCAGAAGC GCATCTGTGA GATGGCAGCC TACTTCACAC ACTCAAATCT GCAGCCTGTG 
CATATGATCT TGGTGCTGCG GACAGCCCTC AACCTTTTCT TCAAGCTCAA GAACTTCAAG 
ACAGCTGCCA CCTTTGCCCG ACGCCTGCTG GAACTTGGGC CCAAGCCTGA AGTAGCTCAG 
CAGACAAGAA AAATCTTATC TGCCTGTGAG AAAAACCCCA CAGATGCCTG CCAGCTCAAT 
TATGACATGC ACAACCCCTT TGACATTTGT GCTGCATCTT ACCGGCCCAT CTACCGTGGA 
AAACCAGTGG AGAAGTGTCC ACTCAGTGGG GCCTGCTATT CCCCTGAGTT CAAGGGGCAA 
ATCTGCAGGG TCACTACAGT GACAGAGATT GGCAAAGATG TGATTGGGCT GAGGATCAGT 
CCTCTTCAGT TTCGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Copa ( NM_009938.4 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_034068.3 Mus musculus coatomer protein complex subunit alpha (Copa), mRNA.

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CloneID	OMu18270
Clone ID Related Accession (Same CDS sequence)	NM_009938.4
Accession Version	NM_009938.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3675bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-27
Organism	Mus musculus(house mouse)
Product	coatomer subunit alpha
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from CF734857.1, AK043479.1, AC158930.3 and AW537456.1. On Apr 15, 2009 this sequence version replaced NM_009938.3. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660817.161731.1, AK043479.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END##

ATGCTAACCA AATTCGAGAC GAAGAGCGCG CGGGTCAAAG GCCTTAGTTT TCATCCTAAA 
AGACCCTGGA TCCTGACCAG TTTACACAAT GGGGTCATCC AGTTATGGGA CTATCGGATG 
TGCACCCTAA TTGACAAATT TGATGAACAT GATGGTCCAG TGCGTGGCAT TGACTTCCAT 
AAGCAGCAGC CACTGTTCGT CTCTGGAGGC GATGATTATA AGATTAAGGT TTGGAATTAC 
AAACTTCGGC GCTGCCTGTT CACATTGCTC GGGCACTTGG ATTATATCCG TACAACGTTT 
TTCCATCATG AATATCCTTG GATTCTGAGC GCCTCAGATG ATCAGACCAT TCGGGTGTGG 
AACTGGCAGT CTAGAACCTG TGTCTGTGTG TTAACGGGGC ACAACCATTA TGTAATGTGT 
GCTCAGTTCC ACCCCTCTGA AGACCTGGTG GTATCAGCTA GCCTGGACCA GACTGTCCGC 
GTTTGGGATA TTTCTGGTCT AAGGAAGAAA AACTTGTCTC CTGGTGCGGT GGAGTCGGAT 
GTGAGAGGAA TAACTGGCGT TGATCTGTTT GGAACCACGG ATGCCGTGGT GAAGCATGTG 
CTAGAGGGTC ATGACCGTGG AGTCAACTGG GCTGCCTTCC ACCCGACTAT GCCCCTTATT 
GTATCTGGGG CAGATGACCG GCAAGTGAAG ATCTGGCGTA TGAATGAATC AAAGGCATGG 
GAGGTTGACA CCTGCCGGGG CCATTACAAC AATGTGTCTT GTGCTGTTTT TCACCCTCGC 
CAAGAGTTGA TCCTCAGCAA TTCTGAAGAC AAGAGTATTC GAGTCTGGGA TATGTCTAAG 
CGGACTGGGG TTCAGACTTT CCGTAGGGAT CATGATCGCT TCTGGGTCCT GGCTGCCCAT 
CCCAACCTTA ACCTCTTTGC AGCAGGCCAT GATGGTGGGA TGATTGTATT TAAGCTGGAA 
CGTGAGCGTC CTGCCTATGC TGTTCATGGT AACATGCTGC ATTATGTAAA GGACCGATTC 
CTCCGTCAGC TGGATTTCAA CAGCTCCAAA GATGTAGCTG TGATGCAGTT GCGGAGCGGT 
TCTAAGTTTC CAGTATTCAA CATGTCATAC AATCCAGCAG AAAACGCAGT GCTGCTCTGC 
ACCAGAGCAA GCAATCTAGA GAACAGTACC TACGACCTAT ACACCATCCC CAAAGACGCA 
GACTCCCAGA ACCCTGACGC CCCTGAAGGG AAACGATCCT CAGGCCTGAC AGCGGTTTGG 
GTTGCTCGGA ATCGGTTTGC TGTCCTAGAT CGCATGCATT CACTTCTGAT TAAAAACCTG 
AAAAATGAGA TCACCAAGAA AATCCAGGTG CCAAACTGTG ATGAAATCTT CTATGCTGGT 
ACAGGCAACC TCCTGCTTCG GGATGCAGAT TCCATCACAC TCTTTGACGT CCAGCAGAAG 
CGAACTCTGG CATCAGTGAA GATTTCCAAG GTGAAATATG TTATCTGGTC GGCAGACATG 
TCTCATGTAG CGTTACTGGC CAAGCATGCC ATTGTGATCT GTAACCGCAA GCTGGATGCT 
CTGTGTAACA TTCATGAGAA CATTCGTGTC AAGAGTGGGG CCTGGGATGA AAGTGGGGTG 
TTTATCTATA CCACAAGCAA CCACATCAAA TATGCTGTCA CCACTGGGGA CCATGGGATT 
ATCCGAACTC TGGATTTACC CATCTATGTC ACCAGAGTGA AGGGCAATAA TGTATACTGC 
CTAGACAGAG AGTGTCGTCC TCGGGTGCTA ACCATTGATC CCACTGAGTT CAAGTTCAAA 
CTGGCCCTGA TCAACAGAAA GTATGATGAG GTACTCCACA TGGTGAGGAA CGCTAAGCTG 
GTAGGCCAGT CAATCATCGC TTATCTCCAG AAGAAGGGCT ATCCTGAAGT GGCGCTGCAT 
TTTGTAAAGG ATGAGAAAAC TCGTTTCAGC CTGGCCCTGG AGTGTGGAAA CATTGAGATT 
GCTCTAGAGG CAGCCAAAGC CCTGGACGAC AAGAACTGCT GGGAGAAGCT AGGAGAAGTG 
GCCCTGCTAC AGGGCAATCA CCAAATTGTA GAGATGTGCT ATCAGCGCAC CAAAAACTTT 
GACAAACTTT CCTTCCTGTA CCTTATTACT GGCAACCTGG AGAAACTTCG AAAGATGATG 
AAGATCGCTG AGATCCGAAA GGACATGAGT GGCCATTATC AGAATGCCCT ATACCTAGGT 
GATGTGTCAG AGAGAGTACG GATCCTGAAG AACTGTGGGC AGAAATCCCT AGCCTATCTC 
TCAGCTGCTA CCCATGGCTT AGATGAAGAA GCTGAGAGCC TGAAGGAAAC CTTTGACCCT 
GAGAAGGAGA CAATCCCAGA CATTGATCCT AATGCCAAGC TGCTCCAGCC ACCTGCACCT 
ATCATGCCAT TGGATACAAA CTGGCCCTTA CTGACCGTGT CCAAAGGGTT CTTTGAAGGC 
TCCATTGCAA GCAAGGGGAA GGGAGGAGCA TTGGCTGCTG ACATTGACAT CGACACTGTA 
GGCACTGAAG GCTGGGGAGA GGACGCAGAG CTGCAGCTGG ATGAAGATGG GTTTGTGGAG 
GCTCCAGAAG GTTTGGGAGA GGATGTTCTT GGAAAGGGAC AAGAAGAAGG AGGTGGCTGG 
GATGTAGAAG AAGATTTGGA GCTCCCACCT GAGCTGGATG TACCCTCTGG GGTGTCTGGT 
AGTGCTGAAG ATGGGTTCTT TGTGCCCCCA ACCAAGGGAA CAAGCCCAAC GCAAATCTGG 
TGCAATAACT CCCAGCTTCC AGTAGATCAC ATCTTGGCAG GTTCTTTTGA AACAGCCATG 
CGGCTTCTCC ATGATCAGGT GGGAGTAATC CAGTTTGGCC CCTATAAGCA ACTGTTCCTA 
CAGACATATG CCCGAGGCCG CACCACCTAT CAAGCGCTGC CCTGCTTGCC CTCCATGTAT 
AGTTACCCTA ATCGCAATTG GAAGGATGCA GGGCTAAAGA ATGGAGTACC AGCTGTAGGC 
CTAAAGCTTA ATGACCTGAT CCAACGTTTG CAACTGTGTT ATCAGCTCAC CACAGTTGGC 
AAGTTTGAGG AAGCTGTGGA AAAATTCCGT TCCATCCTAC TCAGTGTGCC TCTTCTTGTT 
GTGGACAATA AACAGGAGAT TGCAGAGGCC CAGCAGCTCA TCACCATTTG CCGTGAATAC 
ATTGTGGGTT TGTGCATGGA GATAGAAAGG AAGAAGCTAC CTAAGGAGAC CCTAGACCAG 
CAGAAGCGCA TCTGTGAGAT GGCAGCCTAC TTCACACACT CAAATCTGCA GCCTGTGCAT 
ATGATCTTGG TGCTGCGGAC AGCCCTCAAC CTTTTCTTCA AGCTCAAGAA CTTCAAGACA 
GCTGCCACCT TTGCCCGACG CCTGCTGGAA CTTGGGCCCA AGCCTGAAGT AGCTCAGCAG 
ACAAGAAAAA TCTTATCTGC CTGTGAGAAA AACCCCACAG ATGCCTGCCA GCTCAATTAT 
GACATGCACA ACCCCTTTGA CATTTGTGCT GCATCTTACC GGCCCATCTA CCGTGGAAAA 
CCAGTGGAGA AGTGTCCACT CAGTGGGGCC TGCTATTCCC CTGAGTTCAA GGGGCAAATC 
TGCAGGGTCA CTACAGTGAC AGAGATTGGC AAAGATGTGA TTGGGCTGAG GATCAGTCCT 
CTTCAGTTTC GCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_034068.3
CDS	382..4056
Translation	MLTKFETKSARVKGLSFHPKRPWILTSLHNGVIQLWDYRMCTLIDKFDEHDGPVRGIDFHKQQPLFVSGGDDYKIKVWNYKLRRCLFTLLGHLDYIRTTFFHHEYPWILSASDDQTIRVWNWQSRTCVCVLTGHNHYVMCAQFHPSEDLVVSASLDQTVRVWDISGLRKKNLSPGAVESDVRGITGVDLFGTTDAVVKHVLEGHDRGVNWAAFHPTMPLIVSGADDRQVKIWRMNESKAWEVDTCRGHYNNVSCAVFHPRQELILSNSEDKSIRVWDMSKRTGVQTFRRDHDRFWVLAAHPNLNLFAAGHDGGMIVFKLERERPAYAVHGNMLHYVKDRFLRQLDFNSSKDVAVMQLRSGSKFPVFNMSYNPAENAVLLCTRASNLENSTYDLYTIPKDADSQNPDAPEGKRSSGLTAVWVARNRFAVLDRMHSLLIKNLKNEITKKIQVPNCDEIFYAGTGNLLLRDADSITLFDVQQKRTLASVKISKVKYVIWSADMSHVALLAKHAIVICNRKLDALCNIHENIRVKSGAWDESGVFIYTTSNHIKYAVTTGDHGIIRTLDLPIYVTRVKGNNVYCLDRECRPRVLTIDPTEFKFKLALINRKYDEVLHMVRNAKLVGQSIIAYLQKKGYPEVALHFVKDEKTRFSLALECGNIEIALEAAKALDDKNCWEKLGEVALLQGNHQIVEMCYQRTKNFDKLSFLYLITGNLEKLRKMMKIAEIRKDMSGHYQNALYLGDVSERVRILKNCGQKSLAYLSAATHGLDEEAESLKETFDPEKETIPDIDPNAKLLQPPAPIMPLDTNWPLLTVSKGFFEGSIASKGKGGALAADIDIDTVGTEGWGEDAELQLDEDGFVEAPEGLGEDVLGKGQEEGGGWDVEEDLELPPELDVPSGVSGSAEDGFFVPPTKGTSPTQIWCNNSQLPVDHILAGSFETAMRLLHDQVGVIQFGPYKQLFLQTYARGRTTYQALPCLPSMYSYPNRNWKDAGLKNGVPAVGLKLNDLIQRLQLCYQLTTVGKFEEAVEKFRSILLSVPLLVVDNKQEIAEAQQLITICREYIVGLCMEIERKKLPKETLDQQKRICEMAAYFTHSNLQPVHMILVLRTALNLFFKLKNFKTAATFARRLLELGPKPEVAQQTRKILSACEKNPTDACQLNYDMHNPFDICAASYRPIYRGKPVEKCPLSGACYSPEFKGQICRVTTVTEIGKDVIGLRISPLQFR

Target ORF information:

RefSeq Version	NM_009938.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus coatomer protein complex subunit alpha (Copa), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_009938.4

ORF Insert Sequence:

ATGCTAACCA AATTCGAGAC GAAGAGCGCG CGGGTCAAAG GCCTTAGTTT TCATCCTAAA 
AGACCCTGGA TCCTGACCAG TTTACACAAT GGGGTCATCC AGTTATGGGA CTATCGGATG 
TGCACCCTAA TTGACAAATT TGATGAACAT GATGGTCCAG TGCGTGGCAT TGACTTCCAT 
AAGCAGCAGC CACTGTTCGT CTCTGGAGGC GATGATTATA AGATTAAGGT TTGGAATTAC 
AAACTTCGGC GCTGCCTGTT CACATTGCTC GGGCACTTGG ATTATATCCG TACAACGTTT 
TTCCATCATG AATATCCTTG GATTCTGAGC GCCTCAGATG ATCAGACCAT TCGGGTGTGG 
AACTGGCAGT CTAGAACCTG TGTCTGTGTG TTAACGGGGC ACAACCATTA TGTAATGTGT 
GCTCAGTTCC ACCCCTCTGA AGACCTGGTG GTATCAGCTA GCCTGGACCA GACTGTCCGC 
GTTTGGGATA TTTCTGGTCT AAGGAAGAAA AACTTGTCTC CTGGTGCGGT GGAGTCGGAT 
GTGAGAGGAA TAACTGGCGT TGATCTGTTT GGAACCACGG ATGCCGTGGT GAAGCATGTG 
CTAGAGGGTC ATGACCGTGG AGTCAACTGG GCTGCCTTCC ACCCGACTAT GCCCCTTATT 
GTATCTGGGG CAGATGACCG GCAAGTGAAG ATCTGGCGTA TGAATGAATC AAAGGCATGG 
GAGGTTGACA CCTGCCGGGG CCATTACAAC AATGTGTCTT GTGCTGTTTT TCACCCTCGC 
CAAGAGTTGA TCCTCAGCAA TTCTGAAGAC AAGAGTATTC GAGTCTGGGA TATGTCTAAG 
CGGACTGGGG TTCAGACTTT CCGTAGGGAT CATGATCGCT TCTGGGTCCT GGCTGCCCAT 
CCCAACCTTA ACCTCTTTGC AGCAGGCCAT GATGGTGGGA TGATTGTATT TAAGCTGGAA 
CGTGAGCGTC CTGCCTATGC TGTTCATGGT AACATGCTGC ATTATGTAAA GGACCGATTC 
CTCCGTCAGC TGGATTTCAA CAGCTCCAAA GATGTAGCTG TGATGCAGTT GCGGAGCGGT 
TCTAAGTTTC CAGTATTCAA CATGTCATAC AATCCAGCAG AAAACGCAGT GCTGCTCTGC 
ACCAGAGCAA GCAATCTAGA GAACAGTACC TACGACCTAT ACACCATCCC CAAAGACGCA 
GACTCCCAGA ACCCTGACGC CCCTGAAGGG AAACGATCCT CAGGCCTGAC AGCGGTTTGG 
GTTGCTCGGA ATCGGTTTGC TGTCCTAGAT CGCATGCATT CACTTCTGAT TAAAAACCTG 
AAAAATGAGA TCACCAAGAA AATCCAGGTG CCAAACTGTG ATGAAATCTT CTATGCTGGT 
ACAGGCAACC TCCTGCTTCG GGATGCAGAT TCCATCACAC TCTTTGACGT CCAGCAGAAG 
CGAACTCTGG CATCAGTGAA GATTTCCAAG GTGAAATATG TTATCTGGTC GGCAGACATG 
TCTCATGTAG CGTTACTGGC CAAGCATGCC ATTGTGATCT GTAACCGCAA GCTGGATGCT 
CTGTGTAACA TTCATGAGAA CATTCGTGTC AAGAGTGGGG CCTGGGATGA AAGTGGGGTG 
TTTATCTATA CCACAAGCAA CCACATCAAA TATGCTGTCA CCACTGGGGA CCATGGGATT 
ATCCGAACTC TGGATTTACC CATCTATGTC ACCAGAGTGA AGGGCAATAA TGTATACTGC 
CTAGACAGAG AGTGTCGTCC TCGGGTGCTA ACCATTGATC CCACTGAGTT CAAGTTCAAA 
CTGGCCCTGA TCAACAGAAA GTATGATGAG GTACTCCACA TGGTGAGGAA CGCTAAGCTG 
GTAGGCCAGT CAATCATCGC TTATCTCCAG AAGAAGGGCT ATCCTGAAGT GGCGCTGCAT 
TTTGTAAAGG ATGAGAAAAC TCGTTTCAGC CTGGCCCTGG AGTGTGGAAA CATTGAGATT 
GCTCTAGAGG CAGCCAAAGC CCTGGACGAC AAGAACTGCT GGGAGAAGCT AGGAGAAGTG 
GCCCTGCTAC AGGGCAATCA CCAAATTGTA GAGATGTGCT ATCAGCGCAC CAAAAACTTT 
GACAAACTTT CCTTCCTGTA CCTTATTACT GGCAACCTGG AGAAACTTCG AAAGATGATG 
AAGATCGCTG AGATCCGAAA GGACATGAGT GGCCATTATC AGAATGCCCT ATACCTAGGT 
GATGTGTCAG AGAGAGTACG GATCCTGAAG AACTGTGGGC AGAAATCCCT AGCCTATCTC 
TCAGCTGCTA CCCATGGCTT AGATGAAGAA GCTGAGAGCC TGAAGGAAAC CTTTGACCCT 
GAGAAGGAGA CAATCCCAGA CATTGATCCT AATGCCAAGC TGCTCCAGCC ACCTGCACCT 
ATCATGCCAT TGGATACAAA CTGGCCCTTA CTGACCGTGT CCAAAGGGTT CTTTGAAGGC 
TCCATTGCAA GCAAGGGGAA GGGAGGAGCA TTGGCTGCTG ACATTGACAT CGACACTGTA 
GGCACTGAAG GCTGGGGAGA GGACGCAGAG CTGCAGCTGG ATGAAGATGG GTTTGTGGAG 
GCTCCAGAAG GTTTGGGAGA GGATGTTCTT GGAAAGGGAC AAGAAGAAGG AGGTGGCTGG 
GATGTAGAAG AAGATTTGGA GCTCCCACCT GAGCTGGATG TACCCTCTGG GGTGTCTGGT 
AGTGCTGAAG ATGGGTTCTT TGTGCCCCCA ACCAAGGGAA CAAGCCCAAC GCAAATCTGG 
TGCAATAACT CCCAGCTTCC AGTAGATCAC ATCTTGGCAG GTTCTTTTGA AACAGCCATG 
CGGCTTCTCC ATGATCAGGT GGGAGTAATC CAGTTTGGCC CCTATAAGCA ACTGTTCCTA 
CAGACATATG CCCGAGGCCG CACCACCTAT CAAGCGCTGC CCTGCTTGCC CTCCATGTAT 
AGTTACCCTA ATCGCAATTG GAAGGATGCA GGGCTAAAGA ATGGAGTACC AGCTGTAGGC 
CTAAAGCTTA ATGACCTGAT CCAACGTTTG CAACTGTGTT ATCAGCTCAC CACAGTTGGC 
AAGTTTGAGG AAGCTGTGGA AAAATTCCGT TCCATCCTAC TCAGTGTGCC TCTTCTTGTT 
GTGGACAATA AACAGGAGAT TGCAGAGGCC CAGCAGCTCA TCACCATTTG CCGTGAATAC 
ATTGTGGGTT TGTGCATGGA GATAGAAAGG AAGAAGCTAC CTAAGGAGAC CCTAGACCAG 
CAGAAGCGCA TCTGTGAGAT GGCAGCCTAC TTCACACACT CAAATCTGCA GCCTGTGCAT 
ATGATCTTGG TGCTGCGGAC AGCCCTCAAC CTTTTCTTCA AGCTCAAGAA CTTCAAGACA 
GCTGCCACCT TTGCCCGACG CCTGCTGGAA CTTGGGCCCA AGCCTGAAGT AGCTCAGCAG 
ACAAGAAAAA TCTTATCTGC CTGTGAGAAA AACCCCACAG ATGCCTGCCA GCTCAATTAT 
GACATGCACA ACCCCTTTGA CATTTGTGCT GCATCTTACC GGCCCATCTA CCGTGGAAAA 
CCAGTGGAGA AGTGTCCACT CAGTGGGGCC TGCTATTCCC CTGAGTTCAA GGGGCAAATC 
TGCAGGGTCA CTACAGTGAC AGAGATTGGC AAAGATGTGA TTGGGCTGAG GATCAGTCCT 
CTTCAGTTTC GCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Relevance of the COPI complex for Alzheimer's disease progression in vivo.
Proceedings of the National Academy of Sciences of the United States of America113(19)5418-23(2016 May)
Bettayeb K,Hooli BV,Parrado AR,Randolph L,Varotsis D,Aryal S,Gresack J,Tanzi RE,Greengard P,Flajolet M

The COPI vesicle complex binds and moves with survival motor neuron within axons.
Human molecular genetics20(9)1701-11(2011 May)
Peter CJ,Evans M,Thayanithy V,Taniguchi-Ishigaki N,Bach I,Kolpak A,Bassell GJ,Rossoll W,Lorson CL,Bao ZZ,Androphy EJ

Knockdown of COPA, identified by loss-of-function screen, induces apoptosis and suppresses tumor growth in mesothelioma mouse model.
Genomics95(4)210-6(2010 Apr)
Sudo H,Tsuji AB,Sugyo A,Kohda M,Sogawa C,Yoshida C,Harada YN,Hino O,Saga T

Phylogenetic relationships of the seven coat protein subunits of the coatomer complex, and comparative sequence analysis of murine xenin and proxenin.
Biochemical genetics39(5-6)201-11(2001 Jun)
Chow VT,Sakharkar MK,Lim DP,Yeo WM

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Copa cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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