Col7a1 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Col7a1
Entrez Gene ID	12836
Full Name	collagen, type VII, alpha 1
Synonyms	AW209154
General protein information	Preferred Names collagen, type VII, alpha 1 Names collagen alpha-1(VII) chain LC collagen col7a1 long-chain collagen procollagen, type VII, alpha 1
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 9 Map Location: 9 F2\|9 59.63 cM

mRNA and Protein(s)

mRNA	Protein	Name
XM_006511937.2	XP_006512000.1	collagen alpha-1(VII) chain isoform X2
NM_007738.4	NP_031764.2	collagen alpha-1(VII) chain precursor
XM_006511935.3	XP_006511998.1	collagen alpha-1(VII) chain isoform X1
NM_007738.3	NP_031764.2	collagen alpha-1(VII) chain precursor

Pathways from BioSystems

Homologies

Rattus norvegicus (Norway rat)	Col7a1	NP_001100328.1
Bos taurus (cattle)	COL7A1	XP_002697097.2
Mus musculus (house mouse)	Col7a1	NP_031764.2
Gallus gallus (chicken)	COL7A1	XP_425157.4
Xenopus tropicalis (tropical clawed frog)	col7a1	XP_002933125.2
Canis lupus familiaris (dog)	COL7A1	NP_001002980.1
Danio rerio (zebrafish)	col7a1	XP_002662551.3
Homo sapiens (human)	COL7A1	NP_000085.1
Pan troglodytes (chimpanzee)	COL7A1	XP_003950149.1

Gene Ontology
Bibliography

Related articles in PubMed

Impaired lymphoid extracellular matrix impedes antibacterial immunity in epidermolysis bullosa.
Nystr?m A, Bornert O, K?hl T, Gretzmeier C, Thriene K, Dengjel J, Pfister-Wartha A, Kiritsi D, Bruckner-Tuderman L
Proceedings of the National Academy of Sciences of the United States of America(2018 Jan)

p63 exerts spatio-temporal control of palatal epithelial cell fate to prevent cleft palate.
Richardson R, Mitchell K, Hammond NL, Mollo MR, Kouwenhoven EN, Wyatt ND, Donaldson IJ, Zeef L, Burgis T, Blance R, van Heeringen SJ, Stunnenberg HG, Zhou H, Missero C, Romano RA, Sinha S, Dixon MJ, Dixon J
PLoS genetics13(6)e1006828(2017 Jun)

Quantitative proteomic profiling of the extracellular matrix of pancreatic islets during the angiogenic switch and insulinoma progression.
Naba A, Clauser KR, Mani DR, Carr SA, Hynes RO
Scientific reports740495(2017 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

miR-29 Regulates COL7A1 in Recessive Dystrophic Epidermolysis Bullosa, directly through targeting its 3' untranslated region at two distinct seed regions and indirectly through targeting an essential transcription factor required for basal COL7A1 expression, SP1
Title: miR-29 Regulates Type VII Collagen in Recessive Dystrophic Epidermolysis Bullosa.

autoantibodies to COL7, independent of the targeted epitopes, induce blisters both ex vivo and in vivo
Title: Autoantibodies to Multiple Epitopes on the Non-Collagenous-1 Domain of Type VII Collagen Induce Blisters.

The cystine knot is N-terminal to the collagen triple helix in in collagen type VII and IX and therefore probably impedes unfolding of the collagen triple helix from the N terminus.
Title: The cysteine-rich region of type VII collagen is a cystine knot with a new topology.

COL7A1 is required for re-epithelialization through organization of laminin-332 at the dermal-epidermal junction during wound healing.
Title: Collagen VII plays a dual role in wound healing.

COL7 regulates ameloblast differentiation and is essential for the formation of Tomes' processes.
Title: Type VII collagen deficiency causes defective tooth enamel formation due to poor differentiation of ameloblasts.

The following Col7a1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Col7a1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu18740	NM_007738.4 Latest version!	Mus musculus collagen, type VII, alpha 1 (Col7a1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OMu18740	XM_006511935.3 Latest version!	Mus musculus collagen, type VII, alpha 1 (Col7a1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OMu43293	XM_006511937.2 Latest version!	Mus musculus collagen, type VII, alpha 1 (Col7a1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1301.30 $1859.00
View Old Accession Versions >>
OMu18740	NM_007738.3	Mus musculus collagen, type VII, alpha 1 (Col7a1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Col7a1 ( NM_007738.4 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_031764.2 Mus musculus collagen, type VII, alpha 1 (Col7a1), mRNA.

Price & Availability↑

CloneID	OMu18740
Clone ID Related Accession (Same CDS sequence)	NM_007738.4 , XM_006511935.3 , NM_007738.3
Accession Version	NM_007738.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 8835bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2020-01-04
Organism	Mus musculus(house mouse)
Product	collagen alpha-1(VII) chain precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC140383.2. On or before Jun 17, 2019 this sequence version replaced XM_006511935.3, NM_007738.3. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## CDS exon combination :: U32107.1 [ECO:0000331] RNAseq introns :: single sample supports all introns SAMN00849381, SAMN00849382 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## inferred exon combination :: based on alignments, homology ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGAGGCTGC GACTCCTGGT CGCTGCGCTC TGCGCTGCAG AGATCCTGAT GGGAGCGCCG 
GAAGTGTGGG CCCAGCCCAG AGATAGAGTG ACCTGCACAC GCCTTTATGC TGCTGACATC 
GTGTTCTTAC TCGATGGCTC GTCGTCCATT GGCCGCAGCA ACTTTCGAGA AGTGCGAGGA 
TTCCTGGAGG GGCTGGTGCT GCCTTTCTCA GGAGCAGCCA GTGCACAGGG CGTCCGGTTT 
GCCACAGTGC AGTACAGTGA TGATCCACAG ACAGAATTTG GTCTGGATAC ACTTGGCTCT 
GGGAGTGACA CCATCCGAGC CATCCGTGAG CTCAGCTATA AAGGTGGTAA CACTCGCACG 
GGGGCCGCTC TTCACCACGT TTCTGACCGT GTCTTCCTGC CTCGTCTAAC ACGTCCTGGT 
GTCCCCAAGG TCTGCATCCT GATCACAGAT GGGAAATCCC AAGATTTAGT GGACACAGCT 
GCCCAAAAGC TGAAGGGGCA GGGAGTCAAG CTGTTTGCTG TGGGAATCAA GAATGCCGAC 
CCTGAGGAAC TGAAGCGTGT GGCCTCACAG CCAACCAGCG ATTTCTTCTT CTTCGTCAAT 
GACTTCAGCA TCTTGAGGAC CCTGTTGCCT CTCATTTCCA GGAGGGTGTG CACAACTGCT 
GGTGGTGTGC CTGTGACCCT GCCTTCTGAT GACACTCCGT CTGGGCCCCG GGACCTGGTG 
CTCTCTGAGC CAAGTAGCCA ATCCTTGCGA GTACAATGGA CAGCAGCCAG TGGCCCTGTG 
ACTGGCTACA AGGTTCAGTA CACACCTCTG ACAGGGCTAG GGCAGCCACT GCCAAGTGAG 
CGACAGGAGG TGAACATCCC GGCTGGTGAG ACCAGCACGC GTCTCCAGGG TCTCCGGCCA 
CTAACGGACT ACCAAGTGAC TGTGGTTGCC CTCTATGCCA ACAGCATCGG GGAGGCCGTG 
AGTGGGACAG CTCGGACCAC TGCCAAGGAA GGGCTAGAAT TGAGCCTCCA GAACATCACA 
TCGCACAGCC TCCTGGTGGC CTGGAGGAGG GTACCAGGTG CTAATGGCTA CCGTGTAACA 
TGGCGGGACC TCAGTGGTGG GCCAACCCAG CAGCAGGACC TGAGCCCTGG ACAGGGGTCA 
GTGTTTCTAG ATCACTTGGA GCCTGGCACG GACTATGAGG TGACCGTGAG CGCCCTATTT 
GGCCACAGCG TGGGGCCTGC CGCTTCTCTG ACTGCCCGCA CCGCATCTTC TGTTGAGCAA 
ACCCTGCATC CCATCATCTT GAGCCCGACA TCCATCCTCC TTTCCTGGAA CTTGGTGCCT 
GAGGCCCGCG GCTACCGCTT GGAGTGGCGT CGTGAGAGTG GCTTGGAAAC ACCACAGAAA 
GTGGAACTGC CCCCTGATGT GACCCGCCAT CAGCTGGATG GTTTGCAGCC AGGCACTGAG 
TACCGTCTCA CGCTGTATAC TCTGCTGGAG GGCCGTGAGG TGGCCACTCC TGCAACCGTG 
GTTCCTACAG GGCTAGAGCA GCTGGTGAGC CCGGTGATGA ATTTACAAGC CATAGAACTA 
CCTGGGCAGC GGGTGCGAGT GTCTTGGAAC CCAGTCCCTG GGGCCACTGA GTACCGTTTC 
ACTGTACGCA CCACCCAGGG GGTCGAGCGG ACTCTATTGC TTCCTGGGAG CCAGACAACC 
TTTGACTTAG ATGATGTTCG AGCTGGGCTT AGCTACACGG TGCGGGTGTC TGCTCGAGTG 
GGTGCTCAGG AGGGTGATGC CAGCATCCTA ACGATCCACA GAGATCCAGA AGCCCCACTT 
GTTGTCCCAG GACTGCGGGT TGTCGCATCA GATGCAACAA GAATTAGGGT GGCCTGGGGA 
CTTGTCCCTG GAGCCAGTGG ATTTCGGATC AGCTGGAGGA CAGGCAGTGG TCCAGAGTCC 
AGCCGGACGC TGACCCCAGA CTCCACTGTC ACGGACATCC TGGGGTTGCA GCCTAGCACC 
TCTTATCAAG TCGCTGTCTC AGCACTTAGA GGGAGAGAAG AGGGGCCCCC TGTGGTCATC 
GTGGCTAGAA CTGATCCACT GGGCCCAGTG AGGAGAGTCC ACTTGACTCA GGCTGGCAGC 
TCATCTGTCA GCATCACCTG GACTGGGGTT CCCGGTGCCA CAGGATACAG GGTTTCCTGG 
CACTCAGGCC ATGGTCCAGA GAAATCTCTG TTGGTTTCTG GAGATGCCAC GGTGGCTGAA 
ATAGATGGGC TGGAGCCGGA CACAGAGTAT ATAGTGCGTG TGAGAACCCA CGTAGCTGGT 
GTGGATGGAG CCCCCGCCTC TGTGGTTGTG AGGACTGCTC CTGAGCCTGT GGGCAGTGTG 
TCGAAACTGC AAATCCTTAA TGCTTCCAGT GATGTTCTAC GGGTCACTTG GGTAGGAGTC 
CCTGGGGCCA CATCCTATAA ACTGGCGTGG GGACGTAGTG AAGGTGGCCC TATGAAACAC 
CGGATACTCC CAGGAAACAA AGAGTCTGCA GAGATACGGG ATCTCGAAGG AGGAGTCAGC 
TACTCCGTGA GAGTGACGGC GCTTGTTGGA GACCGAGAGG GTGCACCGGT GTCCATTGTC 
ATCACCACAC CGCCTGCGAC CCCAGCACTT CTGGAGACTC TTCAAGTTGT GCAGAGCGGG 
GAGCACTCCT TGAGGCTGCG ATGGGAACCA GTACCCGGAG CACCAGGCTT CCGCCTGCAC 
TGGCAACCTG AGGGTGGCCA GGAACAGTCC CTGACCTTGG GACCTGAGTC TAACAGCTAC 
AACCTGGTTG GTCTGGAGCC AGCAACAAAG TACCAAGTAT GGCTGACTGT CCTGGGCCAG 
ACTGGAGAAG GTCCCCCTAG GAAGGTGACT GCATACACTG AGCCCTCTCA CATCCCGAGC 
ACTGAACTAC GCGTGGTGGA TACCTCTATT GACTCAGTGA CTTTGACCTG GACCCCAGTG 
TCTGGGGCAT CCAGTTATAT CTTGTCCTGG CGGCCGCTCA GAGGAACTGG CCAGGAAGTG 
CCGAGGGCCC CACAGACACT GCCAGGGACC TCAAGCTCCC ACAGAGTGAC AGGGCTGGAA 
CCTGGTATTT CCTATGTTTT CTCTCTGACA CCTATCCAGA GCGGTGTACG AGGTTCTGAG 
ATCTCTGTCA CACAAACACC AGCGTGTTCC CATGGCCCAG TGGACGTGGT GTTCCTCTTG 
CATGCCACTC GAGACAATGC TCACAACGCA GAGGCTGTGA GGAGGGTCCT GGAAAGGCTG 
GTGTCTGCAC TTGGGCCTCT TGGTCCACAG GCTGCTCAGG TCGGCCTGCT GACTTACAGC 
CACCGGCCCT CCCCACTGTT CCCACTGAAT AGTTCCCATG ATCTTGGCAT CATCTTGCGA 
AAGATCCGTG ACATCCCCTA CGTGGACCCA AGTGGTAACA ACCTCGGCAC AGCCGTGACC 
ACAGCTCACA GATACCTCTT AGCATCCAAT GCTCCTGGTC GCCGTCAGCA AGTGCCAGGC 
GTGATGGTCC TGTTAGTGGA TGAGCCTCTG AGAGGGGACA TACTCAGCCC CATTCGTGAG 
GCCCAGACTT CTGGGCTTAA GGTGATGGCA CTGAGTTTGG TGGGAGCTGA CCCAGAGCAG 
CTGCGTCGCT TGGCACCAGG CACGGACCCC ATCCAGAACT TCTTTGCTGT GGATAATGGC 
CCAGGCTTGG ACAGAGCAGT CAGTGATCTG GCTGTAGCCC TGTGTCAGGC AGCTGTGACC 
ATAGAGCCGC AAACAGGGCC TTGTGCTGTG CACTGTCCAA AGGGCCAGAA GGGGGAGCCT 
GGAGTAACGG GACTGCAAGG ACAGGCTGGA CCTCCTGGCC CCCCTGGTCT CCCGGGCAGG 
ACCGGTGCTC CAGGCCCGCA AGGTCCTCCT GGAAGTACCC AGGCAAAAGG CGAGAGGGGC 
TTTCCTGGCC CAGAAGGGCC TCCAGGCAGT CCTGGCCTCC CTGGAGTTCC TGGGTCTCCT 
GGCATTAAGG GCTCCACGGG GCGGCCTGGC CCTCGTGGGG AACAGGGAGA ACGAGGGCCT 
CAAGGTCCAA AGGGGGAGCC GGGTGAGCCT GGGCAAATCA CTGGCGGTGG AGGGCCAGGG 
TTTCCTGGAA AGAAAGGGGA CCCTGGACCT TCGGGGCCCC CTGGATCTCG TGGCCCTGTG 
GGGGACCCAG GACCCCGTGG CCCCCCAGGG CTTCCTGGAA TATCAGTGAA GGGTGACAAA 
GGTGATCGTG GGGAACGGGG TCCCCCGGGA CCAGGCATTG GTGCCAGTGA ACAGGGTGAC 
CCTGGACTTC CGGGGCTTCC TGGAAGCCCT GGACCCCAAG GCCCAGCTGG CCGACCTGGA 
GAGAAGGGAG AAAAGGGTGA CTGTGAGGAT GGAGGTCCAG GCCTCCCAGG ACAGCCTGGG 
CCCCCAGGGG AGCCGGGCCT GCGGGGAGCT CCTGGAATGA CTGGGCCCAA AGGTGATCGG 
GGACTGACGG GGACCCCGGG CGAGCCTGGA GTAAAGGGTG AACGGGGACA TCCTGGTCCC 
GTGGGACCCC AGGGGCTCCC CGGGGCTGCT GGACACCCTG GAGTGGAGGG TCCTGAGGGG 
CCACCAGGAC CCACTGGCCG CCGAGGAGAA AAGGGAGAAC CTGGTCGCCC TGGGGATCCT 
GCAGTGGGTC CTGGTGGTGC TGGAGCCAAA GGAGAAAAGG GAGAGGCTGG GCTCCCTGGG 
CCCAGAGGAG CTTCTGGAAG CAAAGGAGAG CAGGGCGCAC CTGGATTGGC TCTTCCTGGA 
GATCCTGGCC CCAAAGGAGA CCCTGGAGAC AGGGGTCCCA TTGGCCTCAC TGGAAGGGCA 
GGACCCACAG GTGACTCAGG GCCTCCTGGA GAGAAAGGAG AGCCTGGTCG ACCTGGTTCC 
CCAGGACCTG TTGGCCCCCG AGGAAGAGAT GGTGAAGCTG GAGAGAAAGG TGACGAGGGG 
ATCCCGGGTG AGCCAGGTTT GCCTGGAAAA GCAGGTGAAC GTGGCCTCCG GGGGGCACCT 
GGGCCTCGGG GGCCTGTGGG TGAGAAGGGG GACCAGGGAG ATCCTGGAGA AGACGGACGG 
AATGGCAGCC CTGGATCATC TGGACCCAAG GGTGACCGTG GGGAGCCTGG TCCCCCAGGT 
CCCCCTGGAC GGCTGGTGGA TGCAGGCATT GAATCCAGAG ACAAGGGAGA GCCTGGACAA 
GAAGGTCCCC GAGGACCCAA GGGTGACCCT GGGCCCCCTG GAGTCTCTGG AGAAAGGGGC 
ATTGATGGGC TTCGGGGACC CCCAGGCCCA CAGGGAGACC CCGGTGTTCG AGGCCCAGCA 
GGAGACAAGG GTGATCGGGG ACCCCCAGGG CTGGATGGCC GGAGTGGGCT GGATGGGAAA 
CCCGGAGCCC CTGGCCCCCC AGGGCTACAT GGTGCTTCAG GCAAAGCTGG GGACCCGGGG 
AGAGATGGAC TTCCAGGCCT TCGAGGAGAA CATGGCCCCC CTGGTCCCCC TGGCCCTCCT 
GGAGTTCCGG GAAAGGCAGG CGATGATGGC AAACCAGGCC TGAATGGGAA AAACGGAGAC 
CCTGGAGACC CTGGAGAAGA TGGAAGGAAG GGAGAAAAGG GAGATTCGGG TGCCCCTGGA 
AGAGAAGGCC CTGATGGCCC CAAGGGTGAA CGTGGAGCTC CTGGCAATCC TGGACTCCAG 
GGTCCTCCGG GTCTCCCAGG GCAGGTCGGT CCTCCCGGCC AGGGTTTCCC TGGTGTCCCA 
GGAATTACGG GTCCTAAGGG TGACCGTGGA GAGACTGGAT CCAAAGGGGA ACAGGGCCTT 
CCTGGAGAAC GTGGCCTACG AGGAGAACCT GGGAGCTTGC CAAACGCGGA GCGTTTGCTG 
GAAACTGCTG GCATCAAGGT GTCGGCCCTG CGGGAGATCG TGGACACCTG GGATGAGAGT 
TCTGGCAGCT TCCTACCGGT GCCTGAGCGG AGACCTGGCC CCAAGGGGGA CCCTGGTGAC 
CGAGGCCCTC CAGGCAAGGA GGGCCTCATC GGCTTTCCTG GAGAACGTGG ACTGAAGGGA 
GAGCGTGGAG ACCCTGGCCC TCAGGGGCCT CCTGGCCTGG CCCTGGGTGA GAGGGGCCCA 
CCTGGCCCAC CCGGCCTTGC GGGGGAACCC GGAAAGCCTG GCATTCCTGG ACTCCCAGGC 
CGGGCTGGTG GTTCAGGGGA AGCAGGAAGG CCAGGAGAGA GGGGAGAAAG GGGAGAGAAA 
GGAGAACGCG GGGATCAGGG CAGAGATGGC CTTCCCGGCC TCCCTGGACC ACCTGGCCCT 
CCTGGCCCCA AGGTGGCCAT TGAAGAGCCA GGCCCTGGAC TGGCTAGAGA GCAAGGACCT 
CCTGGACTCA AGGGTGCCAA GGGGGAGCCA GGCAGTGATG GTGACCCTGG ACCTAAAGGA 
GACAGGGGTG TACCAGGCAT CAAAGGCGAT GTGGGAGAGC CTGGAAAGAG GGGTCATGAT 
GGCAACCCAG GTCTCCCGGG AGAGCGTGGT GTGGCTGGGC CTGAAGGGAA GCCGGGTCTT 
CAGGGCCCAA GGGGGACCCC TGGCCCAGTG GGTAGCCATG GAGACCCTGG ACCACCTGGT 
GCCCCGGGTC TTGCTGGCCC TGCGGGACCC CAGGGGCCTT CTGGCCTGAA GGGGGAGCCT 
GGAGAGACAG GACCTCCAGG ACGGGGTCTG CCTGGACCTG TTGGAGCTGT GGGACTCCCT 
GGCCCTCCTG GCCCTTCCGG CCTCGTGGGT CCACAAGGCT CTCCAGGTTT GCCCGGACAA 
GTGGGGGAGA CAGGGAAGCC AGGACCTCCA GGCCGTGACG GTAGCAGTGG AAAGGACGGA 
GATCGGGGAA GTCCTGGTGT GCCGGGGTCA CCAGGTCTGC CCGGCCCTGT TGGACCTAAA 
GGAGAACCTG GGCCTGTGGG GGCTCCTGGA CAGGTGGTGG TCGGGCCCCC TGGAGCAAAA 
GGCGAGAAGG GAGCCCCGGG AGACCTTGCT GGAGCCCTGC TGGGTGAGCC GGGAGCCAAA 
GGTGACCGAG GACTGCCTGG ACCACGGGGT GAAAAGGGTG AAGCTGGCCG TGCAGGAGGA 
CCTGGGGACC CTGGGGAAGA TGGTCAAAAA GGGGCTCCAG GACTCAAAGG TCTTAAGGGT 
GAGCCAGGCA TTGGGGTTCA GGGCCCCCCT GGCCCAAGTG GTCCTCCAGG TATGAAGGGA 
GACTTGGGCC CCCCTGGTGC CCCCGGGGCT CCTGGTGTTG TTGGGTTCCC TGGTCAGACA 
GGACCTCGAG GAGAGACGGG CCAGCCAGGG CCTGTTGGAG AGAGGGGTCT GGCAGGCCCT 
CCAGGGAGAG AAGGTGCCCC AGGTCCCTTG GGACCACCTG GACCACCAGG GTCAGCGGGA 
GCACCTGGCG CCTCTGGACT CAAAGGAGAC AAGGGAGACC CGGGAGCAGG ACTGCCGGGG 
CCCCGAGGTG AGCGTGGTGA GCCAGGTGTC CGGGGTGAAG ATGGCCACCC TGGCCAGGAA 
GGACCTCGTG GACTTGTGGG GCCCCCTGGC AGCCGGGGAG AGCAAGGAGA GAAGGGCGCT 
GCTGGCGCTG CAGGGCTCAA GGGTGACAAG GGTGACTCAG CTGTGATTGA GGGACCTCCC 
GGGCCACGAG GTGCCAAAGG GGATATGGGT GAACGAGGGC CTCGGGGCAT AGATGGTGAC 
AAAGGACCTC GGGGAGAAAG TGGGAATCCT GGTGACAAGG GTTCCAAAGG AGAGCCAGGT 
GACAAAGGCT CAGCTGGATC GATTGGAGTT CGAGGACTCA CAGGACCCAA GGGAGAGCCA 
GGTGCTGCAG GGATCCCCGG TGAGCCGGGA GCCCCAGGAA AGGATGGAAT CCCTGGTTTC 
CGAGGAGACA AAGGAGATAT TGGCTTCATG GGTCCCAGGG GCCTCAAGGG TGAAAAAGGA 
ATAAAGGGAA CCTGTGGCCG TGACGGAGAG AGAGGAGATA AGGGAGAAGC TGGTTTTCCT 
GGTCGCCCTG GGTTGGCAGG AAAGAAAGGA GACATGGGGG AGCCTGGTCT GCCGGGTCAG 
TCTGGGGCTC CTGGGAAAGA AGGCCTGATC GGTCCCAAGG GTGACAGAGG CTTTGATGGG 
CAGTCAGGAC CCAAGGGTGA CCAAGGCGAG AAAGGGGAGA GGGGGCCTCC AGGAGTTGGG 
GGCTTCCCAG GTCCCAGAGG AAACGATGGC TCTAGTGGTC CCCCAGGGCC ACCTGGTGGT 
GTTGGTCCCA AGGGACCCGA AGGGCTTCAG GGTCAGAAGG GTGAGCGAGG TCCCCCTGGA 
GAGAGCGTGG TGGGGGCTCC TGGTGCTCCT GGCACCCCTG GAGAGAGAGG AGAGCAGGGA 
CGACCAGGAC CTGCTGGCCC CCGTGGGGAG AAGGGAGAAG CTGCGCTGAC TGAGGATGAC 
ATTCGGGACT TTGTGCGTCA GGAGATGAGT CAGCACTGTG CCTGCCAGGG CCAGTTTATT 
GCGTCTGGAT CACGACCCCT CCCCGGTTAT GCTGCGGATA CAGCTGGTTC CCAGCTTCAC 
CATGTTCCTG TGCTCCGGGT CTCTCATGTT GAGGAGGAAG GCCAGGTGCC CCCTGAAGAT 
GACGATGACT TCTCTGAGTA CTCTGTGTAT TCTGTGGAGG ACTACCAGGA GCCTGAAGTT 
CCATGGGATG GTGAAGCCGA GATTAAGGGC TGGGACCAGA GAGGTTCAGA TCTCTGCTCC 
CTACCCTTGG ATGAGGGCTC CTGTACTGCC TATACCCTGC GCTGGTACCA TCGAGCTGTG 
CCTGGTGGCA CAGCCTGTCA TCCCTTTGTC TATGGTGGCT GCGGAGGGAA TGCCAATCGT 
TTTGGAACCC GTGAGGCGTG TGAGCGCCGC TGCCCACCCC AAGGGGTCCA CAGCCAGAAA 
ACAGGTGCTG CCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_031764.2
CDS	53..8887
Translation	MRLRLLVAALCAAEILMGAPEVWAQPRDRVTCTRLYAADIVFLLDGSSSIGRSNFREVRGFLEGLVLPFSGAASAQGVRFATVQYSDDPQTEFGLDTLGSGSDTIRAIRELSYKGGNTRTGAALHHVSDRVFLPRLTRPGVPKVCILITDGKSQDLVDTAAQKLKGQGVKLFAVGIKNADPEELKRVASQPTSDFFFFVNDFSILRTLLPLISRRVCTTAGGVPVTLPSDDTPSGPRDLVLSEPSSQSLRVQWTAASGPVTGYKVQYTPLTGLGQPLPSERQEVNIPAGETSTRLQGLRPLTDYQVTVVALYANSIGEAVSGTARTTAKEGLELSLQNITSHSLLVAWRRVPGANGYRVTWRDLSGGPTQQQDLSPGQGSVFLDHLEPGTDYEVTVSALFGHSVGPAASLTARTASSVEQTLHPIILSPTSILLSWNLVPEARGYRLEWRRESGLETPQKVELPPDVTRHQLDGLQPGTEYRLTLYTLLEGREVATPATVVPTGLEQLVSPVMNLQAIELPGQRVRVSWNPVPGATEYRFTVRTTQGVERTLLLPGSQTTFDLDDVRAGLSYTVRVSARVGAQEGDASILTIHRDPEAPLVVPGLRVVASDATRIRVAWGLVPGASGFRISWRTGSGPESSRTLTPDSTVTDILGLQPSTSYQVAVSALRGREEGPPVVIVARTDPLGPVRRVHLTQAGSSSVSITWTGVPGATGYRVSWHSGHGPEKSLLVSGDATVAEIDGLEPDTEYIVRVRTHVAGVDGAPASVVVRTAPEPVGSVSKLQILNASSDVLRVTWVGVPGATSYKLAWGRSEGGPMKHRILPGNKESAEIRDLEGGVSYSVRVTALVGDREGAPVSIVITTPPATPALLETLQVVQSGEHSLRLRWEPVPGAPGFRLHWQPEGGQEQSLTLGPESNSYNLVGLEPATKYQVWLTVLGQTGEGPPRKVTAYTEPSHIPSTELRVVDTSIDSVTLTWTPVSGASSYILSWRPLRGTGQEVPRAPQTLPGTSSSHRVTGLEPGISYVFSLTPIQSGVRGSEISVTQTPACSHGPVDVVFLLHATRDNAHNAEAVRRVLERLVSALGPLGPQAAQVGLLTYSHRPSPLFPLNSSHDLGIILRKIRDIPYVDPSGNNLGTAVTTAHRYLLASNAPGRRQQVPGVMVLLVDEPLRGDILSPIREAQTSGLKVMALSLVGADPEQLRRLAPGTDPIQNFFAVDNGPGLDRAVSDLAVALCQAAVTIEPQTGPCAVHCPKGQKGEPGVTGLQGQAGPPGPPGLPGRTGAPGPQGPPGSTQAKGERGFPGPEGPPGSPGLPGVPGSPGIKGSTGRPGPRGEQGERGPQGPKGEPGEPGQITGGGGPGFPGKKGDPGPSGPPGSRGPVGDPGPRGPPGLPGISVKGDKGDRGERGPPGPGIGASEQGDPGLPGLPGSPGPQGPAGRPGEKGEKGDCEDGGPGLPGQPGPPGEPGLRGAPGMTGPKGDRGLTGTPGEPGVKGERGHPGPVGPQGLPGAAGHPGVEGPEGPPGPTGRRGEKGEPGRPGDPAVGPGGAGAKGEKGEAGLPGPRGASGSKGEQGAPGLALPGDPGPKGDPGDRGPIGLTGRAGPTGDSGPPGEKGEPGRPGSPGPVGPRGRDGEAGEKGDEGIPGEPGLPGKAGERGLRGAPGPRGPVGEKGDQGDPGEDGRNGSPGSSGPKGDRGEPGPPGPPGRLVDAGIESRDKGEPGQEGPRGPKGDPGPPGVSGERGIDGLRGPPGPQGDPGVRGPAGDKGDRGPPGLDGRSGLDGKPGAPGPPGLHGASGKAGDPGRDGLPGLRGEHGPPGPPGPPGVPGKAGDDGKPGLNGKNGDPGDPGEDGRKGEKGDSGAPGREGPDGPKGERGAPGNPGLQGPPGLPGQVGPPGQGFPGVPGITGPKGDRGETGSKGEQGLPGERGLRGEPGSLPNAERLLETAGIKVSALREIVDTWDESSGSFLPVPERRPGPKGDPGDRGPPGKEGLIGFPGERGLKGERGDPGPQGPPGLALGERGPPGPPGLAGEPGKPGIPGLPGRAGGSGEAGRPGERGERGEKGERGDQGRDGLPGLPGPPGPPGPKVAIEEPGPGLAREQGPPGLKGAKGEPGSDGDPGPKGDRGVPGIKGDVGEPGKRGHDGNPGLPGERGVAGPEGKPGLQGPRGTPGPVGSHGDPGPPGAPGLAGPAGPQGPSGLKGEPGETGPPGRGLPGPVGAVGLPGPPGPSGLVGPQGSPGLPGQVGETGKPGPPGRDGSSGKDGDRGSPGVPGSPGLPGPVGPKGEPGPVGAPGQVVVGPPGAKGEKGAPGDLAGALLGEPGAKGDRGLPGPRGEKGEAGRAGGPGDPGEDGQKGAPGLKGLKGEPGIGVQGPPGPSGPPGMKGDLGPPGAPGAPGVVGFPGQTGPRGETGQPGPVGERGLAGPPGREGAPGPLGPPGPPGSAGAPGASGLKGDKGDPGAGLPGPRGERGEPGVRGEDGHPGQEGPRGLVGPPGSRGEQGEKGAAGAAGLKGDKGDSAVIEGPPGPRGAKGDMGERGPRGIDGDKGPRGESGNPGDKGSKGEPGDKGSAGSIGVRGLTGPKGEPGAAGIPGEPGAPGKDGIPGFRGDKGDIGFMGPRGLKGEKGIKGTCGRDGERGDKGEAGFPGRPGLAGKKGDMGEPGLPGQSGAPGKEGLIGPKGDRGFDGQSGPKGDQGEKGERGPPGVGGFPGPRGNDGSSGPPGPPGGVGPKGPEGLQGQKGERGPPGESVVGAPGAPGTPGERGEQGRPGPAGPRGEKGEAALTEDDIRDFVRQEMSQHCACQGQFIASGSRPLPGYAADTAGSQLHHVPVLRVSHVEEEGQVPPEDDDDFSEYSVYSVEDYQEPEVPWDGEAEIKGWDQRGSDLCSLPLDEGSCTAYTLRWYHRAVPGGTACHPFVYGGCGGNANRFGTREACERRCPPQGVHSQKTGAA

Target ORF information:

RefSeq Version	NM_007738.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus collagen, type VII, alpha 1 (Col7a1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_007738.4

ORF Insert Sequence:

ATGAGGCTGC GACTCCTGGT CGCTGCGCTC TGCGCTGCAG AGATCCTGAT GGGAGCGCCG 
GAAGTGTGGG CCCAGCCCAG AGATAGAGTG ACCTGCACAC GCCTTTATGC TGCTGACATC 
GTGTTCTTAC TCGATGGCTC GTCGTCCATT GGCCGCAGCA ACTTTCGAGA AGTGCGAGGA 
TTCCTGGAGG GGCTGGTGCT GCCTTTCTCA GGAGCAGCCA GTGCACAGGG CGTCCGGTTT 
GCCACAGTGC AGTACAGTGA TGATCCACAG ACAGAATTTG GTCTGGATAC ACTTGGCTCT 
GGGAGTGACA CCATCCGAGC CATCCGTGAG CTCAGCTATA AAGGTGGTAA CACTCGCACG 
GGGGCCGCTC TTCACCACGT TTCTGACCGT GTCTTCCTGC CTCGTCTAAC ACGTCCTGGT 
GTCCCCAAGG TCTGCATCCT GATCACAGAT GGGAAATCCC AAGATTTAGT GGACACAGCT 
GCCCAAAAGC TGAAGGGGCA GGGAGTCAAG CTGTTTGCTG TGGGAATCAA GAATGCCGAC 
CCTGAGGAAC TGAAGCGTGT GGCCTCACAG CCAACCAGCG ATTTCTTCTT CTTCGTCAAT 
GACTTCAGCA TCTTGAGGAC CCTGTTGCCT CTCATTTCCA GGAGGGTGTG CACAACTGCT 
GGTGGTGTGC CTGTGACCCT GCCTTCTGAT GACACTCCGT CTGGGCCCCG GGACCTGGTG 
CTCTCTGAGC CAAGTAGCCA ATCCTTGCGA GTACAATGGA CAGCAGCCAG TGGCCCTGTG 
ACTGGCTACA AGGTTCAGTA CACACCTCTG ACAGGGCTAG GGCAGCCACT GCCAAGTGAG 
CGACAGGAGG TGAACATCCC GGCTGGTGAG ACCAGCACGC GTCTCCAGGG TCTCCGGCCA 
CTAACGGACT ACCAAGTGAC TGTGGTTGCC CTCTATGCCA ACAGCATCGG GGAGGCCGTG 
AGTGGGACAG CTCGGACCAC TGCCAAGGAA GGGCTAGAAT TGAGCCTCCA GAACATCACA 
TCGCACAGCC TCCTGGTGGC CTGGAGGAGG GTACCAGGTG CTAATGGCTA CCGTGTAACA 
TGGCGGGACC TCAGTGGTGG GCCAACCCAG CAGCAGGACC TGAGCCCTGG ACAGGGGTCA 
GTGTTTCTAG ATCACTTGGA GCCTGGCACG GACTATGAGG TGACCGTGAG CGCCCTATTT 
GGCCACAGCG TGGGGCCTGC CGCTTCTCTG ACTGCCCGCA CCGCATCTTC TGTTGAGCAA 
ACCCTGCATC CCATCATCTT GAGCCCGACA TCCATCCTCC TTTCCTGGAA CTTGGTGCCT 
GAGGCCCGCG GCTACCGCTT GGAGTGGCGT CGTGAGAGTG GCTTGGAAAC ACCACAGAAA 
GTGGAACTGC CCCCTGATGT GACCCGCCAT CAGCTGGATG GTTTGCAGCC AGGCACTGAG 
TACCGTCTCA CGCTGTATAC TCTGCTGGAG GGCCGTGAGG TGGCCACTCC TGCAACCGTG 
GTTCCTACAG GGCTAGAGCA GCTGGTGAGC CCGGTGATGA ATTTACAAGC CATAGAACTA 
CCTGGGCAGC GGGTGCGAGT GTCTTGGAAC CCAGTCCCTG GGGCCACTGA GTACCGTTTC 
ACTGTACGCA CCACCCAGGG GGTCGAGCGG ACTCTATTGC TTCCTGGGAG CCAGACAACC 
TTTGACTTAG ATGATGTTCG AGCTGGGCTT AGCTACACGG TGCGGGTGTC TGCTCGAGTG 
GGTGCTCAGG AGGGTGATGC CAGCATCCTA ACGATCCACA GAGATCCAGA AGCCCCACTT 
GTTGTCCCAG GACTGCGGGT TGTCGCATCA GATGCAACAA GAATTAGGGT GGCCTGGGGA 
CTTGTCCCTG GAGCCAGTGG ATTTCGGATC AGCTGGAGGA CAGGCAGTGG TCCAGAGTCC 
AGCCGGACGC TGACCCCAGA CTCCACTGTC ACGGACATCC TGGGGTTGCA GCCTAGCACC 
TCTTATCAAG TCGCTGTCTC AGCACTTAGA GGGAGAGAAG AGGGGCCCCC TGTGGTCATC 
GTGGCTAGAA CTGATCCACT GGGCCCAGTG AGGAGAGTCC ACTTGACTCA GGCTGGCAGC 
TCATCTGTCA GCATCACCTG GACTGGGGTT CCCGGTGCCA CAGGATACAG GGTTTCCTGG 
CACTCAGGCC ATGGTCCAGA GAAATCTCTG TTGGTTTCTG GAGATGCCAC GGTGGCTGAA 
ATAGATGGGC TGGAGCCGGA CACAGAGTAT ATAGTGCGTG TGAGAACCCA CGTAGCTGGT 
GTGGATGGAG CCCCCGCCTC TGTGGTTGTG AGGACTGCTC CTGAGCCTGT GGGCAGTGTG 
TCGAAACTGC AAATCCTTAA TGCTTCCAGT GATGTTCTAC GGGTCACTTG GGTAGGAGTC 
CCTGGGGCCA CATCCTATAA ACTGGCGTGG GGACGTAGTG AAGGTGGCCC TATGAAACAC 
CGGATACTCC CAGGAAACAA AGAGTCTGCA GAGATACGGG ATCTCGAAGG AGGAGTCAGC 
TACTCCGTGA GAGTGACGGC GCTTGTTGGA GACCGAGAGG GTGCACCGGT GTCCATTGTC 
ATCACCACAC CGCCTGCGAC CCCAGCACTT CTGGAGACTC TTCAAGTTGT GCAGAGCGGG 
GAGCACTCCT TGAGGCTGCG ATGGGAACCA GTACCCGGAG CACCAGGCTT CCGCCTGCAC 
TGGCAACCTG AGGGTGGCCA GGAACAGTCC CTGACCTTGG GACCTGAGTC TAACAGCTAC 
AACCTGGTTG GTCTGGAGCC AGCAACAAAG TACCAAGTAT GGCTGACTGT CCTGGGCCAG 
ACTGGAGAAG GTCCCCCTAG GAAGGTGACT GCATACACTG AGCCCTCTCA CATCCCGAGC 
ACTGAACTAC GCGTGGTGGA TACCTCTATT GACTCAGTGA CTTTGACCTG GACCCCAGTG 
TCTGGGGCAT CCAGTTATAT CTTGTCCTGG CGGCCGCTCA GAGGAACTGG CCAGGAAGTG 
CCGAGGGCCC CACAGACACT GCCAGGGACC TCAAGCTCCC ACAGAGTGAC AGGGCTGGAA 
CCTGGTATTT CCTATGTTTT CTCTCTGACA CCTATCCAGA GCGGTGTACG AGGTTCTGAG 
ATCTCTGTCA CACAAACACC AGCGTGTTCC CATGGCCCAG TGGACGTGGT GTTCCTCTTG 
CATGCCACTC GAGACAATGC TCACAACGCA GAGGCTGTGA GGAGGGTCCT GGAAAGGCTG 
GTGTCTGCAC TTGGGCCTCT TGGTCCACAG GCTGCTCAGG TCGGCCTGCT GACTTACAGC 
CACCGGCCCT CCCCACTGTT CCCACTGAAT AGTTCCCATG ATCTTGGCAT CATCTTGCGA 
AAGATCCGTG ACATCCCCTA CGTGGACCCA AGTGGTAACA ACCTCGGCAC AGCCGTGACC 
ACAGCTCACA GATACCTCTT AGCATCCAAT GCTCCTGGTC GCCGTCAGCA AGTGCCAGGC 
GTGATGGTCC TGTTAGTGGA TGAGCCTCTG AGAGGGGACA TACTCAGCCC CATTCGTGAG 
GCCCAGACTT CTGGGCTTAA GGTGATGGCA CTGAGTTTGG TGGGAGCTGA CCCAGAGCAG 
CTGCGTCGCT TGGCACCAGG CACGGACCCC ATCCAGAACT TCTTTGCTGT GGATAATGGC 
CCAGGCTTGG ACAGAGCAGT CAGTGATCTG GCTGTAGCCC TGTGTCAGGC AGCTGTGACC 
ATAGAGCCGC AAACAGGGCC TTGTGCTGTG CACTGTCCAA AGGGCCAGAA GGGGGAGCCT 
GGAGTAACGG GACTGCAAGG ACAGGCTGGA CCTCCTGGCC CCCCTGGTCT CCCGGGCAGG 
ACCGGTGCTC CAGGCCCGCA AGGTCCTCCT GGAAGTACCC AGGCAAAAGG CGAGAGGGGC 
TTTCCTGGCC CAGAAGGGCC TCCAGGCAGT CCTGGCCTCC CTGGAGTTCC TGGGTCTCCT 
GGCATTAAGG GCTCCACGGG GCGGCCTGGC CCTCGTGGGG AACAGGGAGA ACGAGGGCCT 
CAAGGTCCAA AGGGGGAGCC GGGTGAGCCT GGGCAAATCA CTGGCGGTGG AGGGCCAGGG 
TTTCCTGGAA AGAAAGGGGA CCCTGGACCT TCGGGGCCCC CTGGATCTCG TGGCCCTGTG 
GGGGACCCAG GACCCCGTGG CCCCCCAGGG CTTCCTGGAA TATCAGTGAA GGGTGACAAA 
GGTGATCGTG GGGAACGGGG TCCCCCGGGA CCAGGCATTG GTGCCAGTGA ACAGGGTGAC 
CCTGGACTTC CGGGGCTTCC TGGAAGCCCT GGACCCCAAG GCCCAGCTGG CCGACCTGGA 
GAGAAGGGAG AAAAGGGTGA CTGTGAGGAT GGAGGTCCAG GCCTCCCAGG ACAGCCTGGG 
CCCCCAGGGG AGCCGGGCCT GCGGGGAGCT CCTGGAATGA CTGGGCCCAA AGGTGATCGG 
GGACTGACGG GGACCCCGGG CGAGCCTGGA GTAAAGGGTG AACGGGGACA TCCTGGTCCC 
GTGGGACCCC AGGGGCTCCC CGGGGCTGCT GGACACCCTG GAGTGGAGGG TCCTGAGGGG 
CCACCAGGAC CCACTGGCCG CCGAGGAGAA AAGGGAGAAC CTGGTCGCCC TGGGGATCCT 
GCAGTGGGTC CTGGTGGTGC TGGAGCCAAA GGAGAAAAGG GAGAGGCTGG GCTCCCTGGG 
CCCAGAGGAG CTTCTGGAAG CAAAGGAGAG CAGGGCGCAC CTGGATTGGC TCTTCCTGGA 
GATCCTGGCC CCAAAGGAGA CCCTGGAGAC AGGGGTCCCA TTGGCCTCAC TGGAAGGGCA 
GGACCCACAG GTGACTCAGG GCCTCCTGGA GAGAAAGGAG AGCCTGGTCG ACCTGGTTCC 
CCAGGACCTG TTGGCCCCCG AGGAAGAGAT GGTGAAGCTG GAGAGAAAGG TGACGAGGGG 
ATCCCGGGTG AGCCAGGTTT GCCTGGAAAA GCAGGTGAAC GTGGCCTCCG GGGGGCACCT 
GGGCCTCGGG GGCCTGTGGG TGAGAAGGGG GACCAGGGAG ATCCTGGAGA AGACGGACGG 
AATGGCAGCC CTGGATCATC TGGACCCAAG GGTGACCGTG GGGAGCCTGG TCCCCCAGGT 
CCCCCTGGAC GGCTGGTGGA TGCAGGCATT GAATCCAGAG ACAAGGGAGA GCCTGGACAA 
GAAGGTCCCC GAGGACCCAA GGGTGACCCT GGGCCCCCTG GAGTCTCTGG AGAAAGGGGC 
ATTGATGGGC TTCGGGGACC CCCAGGCCCA CAGGGAGACC CCGGTGTTCG AGGCCCAGCA 
GGAGACAAGG GTGATCGGGG ACCCCCAGGG CTGGATGGCC GGAGTGGGCT GGATGGGAAA 
CCCGGAGCCC CTGGCCCCCC AGGGCTACAT GGTGCTTCAG GCAAAGCTGG GGACCCGGGG 
AGAGATGGAC TTCCAGGCCT TCGAGGAGAA CATGGCCCCC CTGGTCCCCC TGGCCCTCCT 
GGAGTTCCGG GAAAGGCAGG CGATGATGGC AAACCAGGCC TGAATGGGAA AAACGGAGAC 
CCTGGAGACC CTGGAGAAGA TGGAAGGAAG GGAGAAAAGG GAGATTCGGG TGCCCCTGGA 
AGAGAAGGCC CTGATGGCCC CAAGGGTGAA CGTGGAGCTC CTGGCAATCC TGGACTCCAG 
GGTCCTCCGG GTCTCCCAGG GCAGGTCGGT CCTCCCGGCC AGGGTTTCCC TGGTGTCCCA 
GGAATTACGG GTCCTAAGGG TGACCGTGGA GAGACTGGAT CCAAAGGGGA ACAGGGCCTT 
CCTGGAGAAC GTGGCCTACG AGGAGAACCT GGGAGCTTGC CAAACGCGGA GCGTTTGCTG 
GAAACTGCTG GCATCAAGGT GTCGGCCCTG CGGGAGATCG TGGACACCTG GGATGAGAGT 
TCTGGCAGCT TCCTACCGGT GCCTGAGCGG AGACCTGGCC CCAAGGGGGA CCCTGGTGAC 
CGAGGCCCTC CAGGCAAGGA GGGCCTCATC GGCTTTCCTG GAGAACGTGG ACTGAAGGGA 
GAGCGTGGAG ACCCTGGCCC TCAGGGGCCT CCTGGCCTGG CCCTGGGTGA GAGGGGCCCA 
CCTGGCCCAC CCGGCCTTGC GGGGGAACCC GGAAAGCCTG GCATTCCTGG ACTCCCAGGC 
CGGGCTGGTG GTTCAGGGGA AGCAGGAAGG CCAGGAGAGA GGGGAGAAAG GGGAGAGAAA 
GGAGAACGCG GGGATCAGGG CAGAGATGGC CTTCCCGGCC TCCCTGGACC ACCTGGCCCT 
CCTGGCCCCA AGGTGGCCAT TGAAGAGCCA GGCCCTGGAC TGGCTAGAGA GCAAGGACCT 
CCTGGACTCA AGGGTGCCAA GGGGGAGCCA GGCAGTGATG GTGACCCTGG ACCTAAAGGA 
GACAGGGGTG TACCAGGCAT CAAAGGCGAT GTGGGAGAGC CTGGAAAGAG GGGTCATGAT 
GGCAACCCAG GTCTCCCGGG AGAGCGTGGT GTGGCTGGGC CTGAAGGGAA GCCGGGTCTT 
CAGGGCCCAA GGGGGACCCC TGGCCCAGTG GGTAGCCATG GAGACCCTGG ACCACCTGGT 
GCCCCGGGTC TTGCTGGCCC TGCGGGACCC CAGGGGCCTT CTGGCCTGAA GGGGGAGCCT 
GGAGAGACAG GACCTCCAGG ACGGGGTCTG CCTGGACCTG TTGGAGCTGT GGGACTCCCT 
GGCCCTCCTG GCCCTTCCGG CCTCGTGGGT CCACAAGGCT CTCCAGGTTT GCCCGGACAA 
GTGGGGGAGA CAGGGAAGCC AGGACCTCCA GGCCGTGACG GTAGCAGTGG AAAGGACGGA 
GATCGGGGAA GTCCTGGTGT GCCGGGGTCA CCAGGTCTGC CCGGCCCTGT TGGACCTAAA 
GGAGAACCTG GGCCTGTGGG GGCTCCTGGA CAGGTGGTGG TCGGGCCCCC TGGAGCAAAA 
GGCGAGAAGG GAGCCCCGGG AGACCTTGCT GGAGCCCTGC TGGGTGAGCC GGGAGCCAAA 
GGTGACCGAG GACTGCCTGG ACCACGGGGT GAAAAGGGTG AAGCTGGCCG TGCAGGAGGA 
CCTGGGGACC CTGGGGAAGA TGGTCAAAAA GGGGCTCCAG GACTCAAAGG TCTTAAGGGT 
GAGCCAGGCA TTGGGGTTCA GGGCCCCCCT GGCCCAAGTG GTCCTCCAGG TATGAAGGGA 
GACTTGGGCC CCCCTGGTGC CCCCGGGGCT CCTGGTGTTG TTGGGTTCCC TGGTCAGACA 
GGACCTCGAG GAGAGACGGG CCAGCCAGGG CCTGTTGGAG AGAGGGGTCT GGCAGGCCCT 
CCAGGGAGAG AAGGTGCCCC AGGTCCCTTG GGACCACCTG GACCACCAGG GTCAGCGGGA 
GCACCTGGCG CCTCTGGACT CAAAGGAGAC AAGGGAGACC CGGGAGCAGG ACTGCCGGGG 
CCCCGAGGTG AGCGTGGTGA GCCAGGTGTC CGGGGTGAAG ATGGCCACCC TGGCCAGGAA 
GGACCTCGTG GACTTGTGGG GCCCCCTGGC AGCCGGGGAG AGCAAGGAGA GAAGGGCGCT 
GCTGGCGCTG CAGGGCTCAA GGGTGACAAG GGTGACTCAG CTGTGATTGA GGGACCTCCC 
GGGCCACGAG GTGCCAAAGG GGATATGGGT GAACGAGGGC CTCGGGGCAT AGATGGTGAC 
AAAGGACCTC GGGGAGAAAG TGGGAATCCT GGTGACAAGG GTTCCAAAGG AGAGCCAGGT 
GACAAAGGCT CAGCTGGATC GATTGGAGTT CGAGGACTCA CAGGACCCAA GGGAGAGCCA 
GGTGCTGCAG GGATCCCCGG TGAGCCGGGA GCCCCAGGAA AGGATGGAAT CCCTGGTTTC 
CGAGGAGACA AAGGAGATAT TGGCTTCATG GGTCCCAGGG GCCTCAAGGG TGAAAAAGGA 
ATAAAGGGAA CCTGTGGCCG TGACGGAGAG AGAGGAGATA AGGGAGAAGC TGGTTTTCCT 
GGTCGCCCTG GGTTGGCAGG AAAGAAAGGA GACATGGGGG AGCCTGGTCT GCCGGGTCAG 
TCTGGGGCTC CTGGGAAAGA AGGCCTGATC GGTCCCAAGG GTGACAGAGG CTTTGATGGG 
CAGTCAGGAC CCAAGGGTGA CCAAGGCGAG AAAGGGGAGA GGGGGCCTCC AGGAGTTGGG 
GGCTTCCCAG GTCCCAGAGG AAACGATGGC TCTAGTGGTC CCCCAGGGCC ACCTGGTGGT 
GTTGGTCCCA AGGGACCCGA AGGGCTTCAG GGTCAGAAGG GTGAGCGAGG TCCCCCTGGA 
GAGAGCGTGG TGGGGGCTCC TGGTGCTCCT GGCACCCCTG GAGAGAGAGG AGAGCAGGGA 
CGACCAGGAC CTGCTGGCCC CCGTGGGGAG AAGGGAGAAG CTGCGCTGAC TGAGGATGAC 
ATTCGGGACT TTGTGCGTCA GGAGATGAGT CAGCACTGTG CCTGCCAGGG CCAGTTTATT 
GCGTCTGGAT CACGACCCCT CCCCGGTTAT GCTGCGGATA CAGCTGGTTC CCAGCTTCAC 
CATGTTCCTG TGCTCCGGGT CTCTCATGTT GAGGAGGAAG GCCAGGTGCC CCCTGAAGAT 
GACGATGACT TCTCTGAGTA CTCTGTGTAT TCTGTGGAGG ACTACCAGGA GCCTGAAGTT 
CCATGGGATG GTGAAGCCGA GATTAAGGGC TGGGACCAGA GAGGTTCAGA TCTCTGCTCC 
CTACCCTTGG ATGAGGGCTC CTGTACTGCC TATACCCTGC GCTGGTACCA TCGAGCTGTG 
CCTGGTGGCA CAGCCTGTCA TCCCTTTGTC TATGGTGGCT GCGGAGGGAA TGCCAATCGT 
TTTGGAACCC GTGAGGCGTG TGAGCGCCGC TGCCCACCCC AAGGGGTCCA CAGCCAGAAA 
ACAGGTGCTG CCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Col7a1 ( NM_007738.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_031764.2 Mus musculus collagen, type VII, alpha 1 (Col7a1), mRNA.

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CloneID	OMu18740
Clone ID Related Accession (Same CDS sequence)	NM_007738.4 , XM_006511935.3 , NM_007738.3
Accession Version	NM_007738.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 8835bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-08
Organism	Mus musculus(house mouse)
Product	collagen alpha-1(VII) chain precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from U32107.1, AC174646.2, CJ045709.1, CJ045811.1, BI872152.1 and CX224636.1. On Oct 7, 2006 this sequence version replaced NM_007738.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: U32107.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849381, SAMN00849382 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAGGCTGC GACTCCTGGT CGCTGCGCTC TGCGCTGCAG AGATCCTGAT GGGAGCGCCG 
GAAGTGTGGG CCCAGCCCAG AGATAGAGTG ACCTGCACAC GCCTTTATGC TGCTGACATC 
GTGTTCTTAC TCGATGGCTC GTCGTCCATT GGCCGCAGCA ACTTTCGAGA AGTGCGAGGA 
TTCCTGGAGG GGCTGGTGCT GCCTTTCTCA GGAGCAGCCA GTGCACAGGG CGTCCGGTTT 
GCCACAGTGC AGTACAGTGA TGATCCACAG ACAGAATTTG GTCTGGATAC ACTTGGCTCT 
GGGAGTGACA CCATCCGAGC CATCCGTGAG CTCAGCTATA AAGGTGGTAA CACTCGCACG 
GGGGCCGCTC TTCACCACGT TTCTGACCGT GTCTTCCTGC CTCGTCTAAC ACGTCCTGGT 
GTCCCCAAGG TCTGCATCCT GATCACAGAT GGGAAATCCC AAGATTTAGT GGACACAGCT 
GCCCAAAAGC TGAAGGGGCA GGGAGTCAAG CTGTTTGCTG TGGGAATCAA GAATGCCGAC 
CCTGAGGAAC TGAAGCGTGT GGCCTCACAG CCAACCAGCG ATTTCTTCTT CTTCGTCAAT 
GACTTCAGCA TCTTGAGGAC CCTGTTGCCT CTCATTTCCA GGAGGGTGTG CACAACTGCT 
GGTGGTGTGC CTGTGACCCT GCCTTCTGAT GACACTCCGT CTGGGCCCCG GGACCTGGTG 
CTCTCTGAGC CAAGTAGCCA ATCCTTGCGA GTACAATGGA CAGCAGCCAG TGGCCCTGTG 
ACTGGCTACA AGGTTCAGTA CACACCTCTG ACAGGGCTAG GGCAGCCACT GCCAAGTGAG 
CGACAGGAGG TGAACATCCC GGCTGGTGAG ACCAGCACGC GTCTCCAGGG TCTCCGGCCA 
CTAACGGACT ACCAAGTGAC TGTGGTTGCC CTCTATGCCA ACAGCATCGG GGAGGCCGTG 
AGTGGGACAG CTCGGACCAC TGCCAAGGAA GGGCTAGAAT TGAGCCTCCA GAACATCACA 
TCGCACAGCC TCCTGGTGGC CTGGAGGAGG GTACCAGGTG CTAATGGCTA CCGTGTAACA 
TGGCGGGACC TCAGTGGTGG GCCAACCCAG CAGCAGGACC TGAGCCCTGG ACAGGGGTCA 
GTGTTTCTAG ATCACTTGGA GCCTGGCACG GACTATGAGG TGACCGTGAG CGCCCTATTT 
GGCCACAGCG TGGGGCCTGC CGCTTCTCTG ACTGCCCGCA CCGCATCTTC TGTTGAGCAA 
ACCCTGCATC CCATCATCTT GAGCCCGACA TCCATCCTCC TTTCCTGGAA CTTGGTGCCT 
GAGGCCCGCG GCTACCGCTT GGAGTGGCGT CGTGAGAGTG GCTTGGAAAC ACCACAGAAA 
GTGGAACTGC CCCCTGATGT GACCCGCCAT CAGCTGGATG GTTTGCAGCC AGGCACTGAG 
TACCGTCTCA CGCTGTATAC TCTGCTGGAG GGCCGTGAGG TGGCCACTCC TGCAACCGTG 
GTTCCTACAG GGCTAGAGCA GCTGGTGAGC CCGGTGATGA ATTTACAAGC CATAGAACTA 
CCTGGGCAGC GGGTGCGAGT GTCTTGGAAC CCAGTCCCTG GGGCCACTGA GTACCGTTTC 
ACTGTACGCA CCACCCAGGG GGTCGAGCGG ACTCTATTGC TTCCTGGGAG CCAGACAACC 
TTTGACTTAG ATGATGTTCG AGCTGGGCTT AGCTACACGG TGCGGGTGTC TGCTCGAGTG 
GGTGCTCAGG AGGGTGATGC CAGCATCCTA ACGATCCACA GAGATCCAGA AGCCCCACTT 
GTTGTCCCAG GACTGCGGGT TGTCGCATCA GATGCAACAA GAATTAGGGT GGCCTGGGGA 
CTTGTCCCTG GAGCCAGTGG ATTTCGGATC AGCTGGAGGA CAGGCAGTGG TCCAGAGTCC 
AGCCGGACGC TGACCCCAGA CTCCACTGTC ACGGACATCC TGGGGTTGCA GCCTAGCACC 
TCTTATCAAG TCGCTGTCTC AGCACTTAGA GGGAGAGAAG AGGGGCCCCC TGTGGTCATC 
GTGGCTAGAA CTGATCCACT GGGCCCAGTG AGGAGAGTCC ACTTGACTCA GGCTGGCAGC 
TCATCTGTCA GCATCACCTG GACTGGGGTT CCCGGTGCCA CAGGATACAG GGTTTCCTGG 
CACTCAGGCC ATGGTCCAGA GAAATCTCTG TTGGTTTCTG GAGATGCCAC GGTGGCTGAA 
ATAGATGGGC TGGAGCCGGA CACAGAGTAT ATAGTGCGTG TGAGAACCCA CGTAGCTGGT 
GTGGATGGAG CCCCCGCCTC TGTGGTTGTG AGGACTGCTC CTGAGCCTGT GGGCAGTGTG 
TCGAAACTGC AAATCCTTAA TGCTTCCAGT GATGTTCTAC GGGTCACTTG GGTAGGAGTC 
CCTGGGGCCA CATCCTATAA ACTGGCGTGG GGACGTAGTG AAGGTGGCCC TATGAAACAC 
CGGATACTCC CAGGAAACAA AGAGTCTGCA GAGATACGGG ATCTCGAAGG AGGAGTCAGC 
TACTCCGTGA GAGTGACGGC GCTTGTTGGA GACCGAGAGG GTGCACCGGT GTCCATTGTC 
ATCACCACAC CGCCTGCGAC CCCAGCACTT CTGGAGACTC TTCAAGTTGT GCAGAGCGGG 
GAGCACTCCT TGAGGCTGCG ATGGGAACCA GTACCCGGAG CACCAGGCTT CCGCCTGCAC 
TGGCAACCTG AGGGTGGCCA GGAACAGTCC CTGACCTTGG GACCTGAGTC TAACAGCTAC 
AACCTGGTTG GTCTGGAGCC AGCAACAAAG TACCAAGTAT GGCTGACTGT CCTGGGCCAG 
ACTGGAGAAG GTCCCCCTAG GAAGGTGACT GCATACACTG AGCCCTCTCA CATCCCGAGC 
ACTGAACTAC GCGTGGTGGA TACCTCTATT GACTCAGTGA CTTTGACCTG GACCCCAGTG 
TCTGGGGCAT CCAGTTATAT CTTGTCCTGG CGGCCGCTCA GAGGAACTGG CCAGGAAGTG 
CCGAGGGCCC CACAGACACT GCCAGGGACC TCAAGCTCCC ACAGAGTGAC AGGGCTGGAA 
CCTGGTATTT CCTATGTTTT CTCTCTGACA CCTATCCAGA GCGGTGTACG AGGTTCTGAG 
ATCTCTGTCA CACAAACACC AGCGTGTTCC CATGGCCCAG TGGACGTGGT GTTCCTCTTG 
CATGCCACTC GAGACAATGC TCACAACGCA GAGGCTGTGA GGAGGGTCCT GGAAAGGCTG 
GTGTCTGCAC TTGGGCCTCT TGGTCCACAG GCTGCTCAGG TCGGCCTGCT GACTTACAGC 
CACCGGCCCT CCCCACTGTT CCCACTGAAT AGTTCCCATG ATCTTGGCAT CATCTTGCGA 
AAGATCCGTG ACATCCCCTA CGTGGACCCA AGTGGTAACA ACCTCGGCAC AGCCGTGACC 
ACAGCTCACA GATACCTCTT AGCATCCAAT GCTCCTGGTC GCCGTCAGCA AGTGCCAGGC 
GTGATGGTCC TGTTAGTGGA TGAGCCTCTG AGAGGGGACA TACTCAGCCC CATTCGTGAG 
GCCCAGACTT CTGGGCTTAA GGTGATGGCA CTGAGTTTGG TGGGAGCTGA CCCAGAGCAG 
CTGCGTCGCT TGGCACCAGG CACGGACCCC ATCCAGAACT TCTTTGCTGT GGATAATGGC 
CCAGGCTTGG ACAGAGCAGT CAGTGATCTG GCTGTAGCCC TGTGTCAGGC AGCTGTGACC 
ATAGAGCCGC AAACAGGGCC TTGTGCTGTG CACTGTCCAA AGGGCCAGAA GGGGGAGCCT 
GGAGTAACGG GACTGCAAGG ACAGGCTGGA CCTCCTGGCC CCCCTGGTCT CCCGGGCAGG 
ACCGGTGCTC CAGGCCCGCA AGGTCCTCCT GGAAGTACCC AGGCAAAAGG CGAGAGGGGC 
TTTCCTGGCC CAGAAGGGCC TCCAGGCAGT CCTGGCCTCC CTGGAGTTCC TGGGTCTCCT 
GGCATTAAGG GCTCCACGGG GCGGCCTGGC CCTCGTGGGG AACAGGGAGA ACGAGGGCCT 
CAAGGTCCAA AGGGGGAGCC GGGTGAGCCT GGGCAAATCA CTGGCGGTGG AGGGCCAGGG 
TTTCCTGGAA AGAAAGGGGA CCCTGGACCT TCGGGGCCCC CTGGATCTCG TGGCCCTGTG 
GGGGACCCAG GACCCCGTGG CCCCCCAGGG CTTCCTGGAA TATCAGTGAA GGGTGACAAA 
GGTGATCGTG GGGAACGGGG TCCCCCGGGA CCAGGCATTG GTGCCAGTGA ACAGGGTGAC 
CCTGGACTTC CGGGGCTTCC TGGAAGCCCT GGACCCCAAG GCCCAGCTGG CCGACCTGGA 
GAGAAGGGAG AAAAGGGTGA CTGTGAGGAT GGAGGTCCAG GCCTCCCAGG ACAGCCTGGG 
CCCCCAGGGG AGCCGGGCCT GCGGGGAGCT CCTGGAATGA CTGGGCCCAA AGGTGATCGG 
GGACTGACGG GGACCCCGGG CGAGCCTGGA GTAAAGGGTG AACGGGGACA TCCTGGTCCC 
GTGGGACCCC AGGGGCTCCC CGGGGCTGCT GGACACCCTG GAGTGGAGGG TCCTGAGGGG 
CCACCAGGAC CCACTGGCCG CCGAGGAGAA AAGGGAGAAC CTGGTCGCCC TGGGGATCCT 
GCAGTGGGTC CTGGTGGTGC TGGAGCCAAA GGAGAAAAGG GAGAGGCTGG GCTCCCTGGG 
CCCAGAGGAG CTTCTGGAAG CAAAGGAGAG CAGGGCGCAC CTGGATTGGC TCTTCCTGGA 
GATCCTGGCC CCAAAGGAGA CCCTGGAGAC AGGGGTCCCA TTGGCCTCAC TGGAAGGGCA 
GGACCCACAG GTGACTCAGG GCCTCCTGGA GAGAAAGGAG AGCCTGGTCG ACCTGGTTCC 
CCAGGACCTG TTGGCCCCCG AGGAAGAGAT GGTGAAGCTG GAGAGAAAGG TGACGAGGGG 
ATCCCGGGTG AGCCAGGTTT GCCTGGAAAA GCAGGTGAAC GTGGCCTCCG GGGGGCACCT 
GGGCCTCGGG GGCCTGTGGG TGAGAAGGGG GACCAGGGAG ATCCTGGAGA AGACGGACGG 
AATGGCAGCC CTGGATCATC TGGACCCAAG GGTGACCGTG GGGAGCCTGG TCCCCCAGGT 
CCCCCTGGAC GGCTGGTGGA TGCAGGCATT GAATCCAGAG ACAAGGGAGA GCCTGGACAA 
GAAGGTCCCC GAGGACCCAA GGGTGACCCT GGGCCCCCTG GAGTCTCTGG AGAAAGGGGC 
ATTGATGGGC TTCGGGGACC CCCAGGCCCA CAGGGAGACC CCGGTGTTCG AGGCCCAGCA 
GGAGACAAGG GTGATCGGGG ACCCCCAGGG CTGGATGGCC GGAGTGGGCT GGATGGGAAA 
CCCGGAGCCC CTGGCCCCCC AGGGCTACAT GGTGCTTCAG GCAAAGCTGG GGACCCGGGG 
AGAGATGGAC TTCCAGGCCT TCGAGGAGAA CATGGCCCCC CTGGTCCCCC TGGCCCTCCT 
GGAGTTCCGG GAAAGGCAGG CGATGATGGC AAACCAGGCC TGAATGGGAA AAACGGAGAC 
CCTGGAGACC CTGGAGAAGA TGGAAGGAAG GGAGAAAAGG GAGATTCGGG TGCCCCTGGA 
AGAGAAGGCC CTGATGGCCC CAAGGGTGAA CGTGGAGCTC CTGGCAATCC TGGACTCCAG 
GGTCCTCCGG GTCTCCCAGG GCAGGTCGGT CCTCCCGGCC AGGGTTTCCC TGGTGTCCCA 
GGAATTACGG GTCCTAAGGG TGACCGTGGA GAGACTGGAT CCAAAGGGGA ACAGGGCCTT 
CCTGGAGAAC GTGGCCTACG AGGAGAACCT GGGAGCTTGC CAAACGCGGA GCGTTTGCTG 
GAAACTGCTG GCATCAAGGT GTCGGCCCTG CGGGAGATCG TGGACACCTG GGATGAGAGT 
TCTGGCAGCT TCCTACCGGT GCCTGAGCGG AGACCTGGCC CCAAGGGGGA CCCTGGTGAC 
CGAGGCCCTC CAGGCAAGGA GGGCCTCATC GGCTTTCCTG GAGAACGTGG ACTGAAGGGA 
GAGCGTGGAG ACCCTGGCCC TCAGGGGCCT CCTGGCCTGG CCCTGGGTGA GAGGGGCCCA 
CCTGGCCCAC CCGGCCTTGC GGGGGAACCC GGAAAGCCTG GCATTCCTGG ACTCCCAGGC 
CGGGCTGGTG GTTCAGGGGA AGCAGGAAGG CCAGGAGAGA GGGGAGAAAG GGGAGAGAAA 
GGAGAACGCG GGGATCAGGG CAGAGATGGC CTTCCCGGCC TCCCTGGACC ACCTGGCCCT 
CCTGGCCCCA AGGTGGCCAT TGAAGAGCCA GGCCCTGGAC TGGCTAGAGA GCAAGGACCT 
CCTGGACTCA AGGGTGCCAA GGGGGAGCCA GGCAGTGATG GTGACCCTGG ACCTAAAGGA 
GACAGGGGTG TACCAGGCAT CAAAGGCGAT GTGGGAGAGC CTGGAAAGAG GGGTCATGAT 
GGCAACCCAG GTCTCCCGGG AGAGCGTGGT GTGGCTGGGC CTGAAGGGAA GCCGGGTCTT 
CAGGGCCCAA GGGGGACCCC TGGCCCAGTG GGTAGCCATG GAGACCCTGG ACCACCTGGT 
GCCCCGGGTC TTGCTGGCCC TGCGGGACCC CAGGGGCCTT CTGGCCTGAA GGGGGAGCCT 
GGAGAGACAG GACCTCCAGG ACGGGGTCTG CCTGGACCTG TTGGAGCTGT GGGACTCCCT 
GGCCCTCCTG GCCCTTCCGG CCTCGTGGGT CCACAAGGCT CTCCAGGTTT GCCCGGACAA 
GTGGGGGAGA CAGGGAAGCC AGGACCTCCA GGCCGTGACG GTAGCAGTGG AAAGGACGGA 
GATCGGGGAA GTCCTGGTGT GCCGGGGTCA CCAGGTCTGC CCGGCCCTGT TGGACCTAAA 
GGAGAACCTG GGCCTGTGGG GGCTCCTGGA CAGGTGGTGG TCGGGCCCCC TGGAGCAAAA 
GGCGAGAAGG GAGCCCCGGG AGACCTTGCT GGAGCCCTGC TGGGTGAGCC GGGAGCCAAA 
GGTGACCGAG GACTGCCTGG ACCACGGGGT GAAAAGGGTG AAGCTGGCCG TGCAGGAGGA 
CCTGGGGACC CTGGGGAAGA TGGTCAAAAA GGGGCTCCAG GACTCAAAGG TCTTAAGGGT 
GAGCCAGGCA TTGGGGTTCA GGGCCCCCCT GGCCCAAGTG GTCCTCCAGG TATGAAGGGA 
GACTTGGGCC CCCCTGGTGC CCCCGGGGCT CCTGGTGTTG TTGGGTTCCC TGGTCAGACA 
GGACCTCGAG GAGAGACGGG CCAGCCAGGG CCTGTTGGAG AGAGGGGTCT GGCAGGCCCT 
CCAGGGAGAG AAGGTGCCCC AGGTCCCTTG GGACCACCTG GACCACCAGG GTCAGCGGGA 
GCACCTGGCG CCTCTGGACT CAAAGGAGAC AAGGGAGACC CGGGAGCAGG ACTGCCGGGG 
CCCCGAGGTG AGCGTGGTGA GCCAGGTGTC CGGGGTGAAG ATGGCCACCC TGGCCAGGAA 
GGACCTCGTG GACTTGTGGG GCCCCCTGGC AGCCGGGGAG AGCAAGGAGA GAAGGGCGCT 
GCTGGCGCTG CAGGGCTCAA GGGTGACAAG GGTGACTCAG CTGTGATTGA GGGACCTCCC 
GGGCCACGAG GTGCCAAAGG GGATATGGGT GAACGAGGGC CTCGGGGCAT AGATGGTGAC 
AAAGGACCTC GGGGAGAAAG TGGGAATCCT GGTGACAAGG GTTCCAAAGG AGAGCCAGGT 
GACAAAGGCT CAGCTGGATC GATTGGAGTT CGAGGACTCA CAGGACCCAA GGGAGAGCCA 
GGTGCTGCAG GGATCCCCGG TGAGCCGGGA GCCCCAGGAA AGGATGGAAT CCCTGGTTTC 
CGAGGAGACA AAGGAGATAT TGGCTTCATG GGTCCCAGGG GCCTCAAGGG TGAAAAAGGA 
ATAAAGGGAA CCTGTGGCCG TGACGGAGAG AGAGGAGATA AGGGAGAAGC TGGTTTTCCT 
GGTCGCCCTG GGTTGGCAGG AAAGAAAGGA GACATGGGGG AGCCTGGTCT GCCGGGTCAG 
TCTGGGGCTC CTGGGAAAGA AGGCCTGATC GGTCCCAAGG GTGACAGAGG CTTTGATGGG 
CAGTCAGGAC CCAAGGGTGA CCAAGGCGAG AAAGGGGAGA GGGGGCCTCC AGGAGTTGGG 
GGCTTCCCAG GTCCCAGAGG AAACGATGGC TCTAGTGGTC CCCCAGGGCC ACCTGGTGGT 
GTTGGTCCCA AGGGACCCGA AGGGCTTCAG GGTCAGAAGG GTGAGCGAGG TCCCCCTGGA 
GAGAGCGTGG TGGGGGCTCC TGGTGCTCCT GGCACCCCTG GAGAGAGAGG AGAGCAGGGA 
CGACCAGGAC CTGCTGGCCC CCGTGGGGAG AAGGGAGAAG CTGCGCTGAC TGAGGATGAC 
ATTCGGGACT TTGTGCGTCA GGAGATGAGT CAGCACTGTG CCTGCCAGGG CCAGTTTATT 
GCGTCTGGAT CACGACCCCT CCCCGGTTAT GCTGCGGATA CAGCTGGTTC CCAGCTTCAC 
CATGTTCCTG TGCTCCGGGT CTCTCATGTT GAGGAGGAAG GCCAGGTGCC CCCTGAAGAT 
GACGATGACT TCTCTGAGTA CTCTGTGTAT TCTGTGGAGG ACTACCAGGA GCCTGAAGTT 
CCATGGGATG GTGAAGCCGA GATTAAGGGC TGGGACCAGA GAGGTTCAGA TCTCTGCTCC 
CTACCCTTGG ATGAGGGCTC CTGTACTGCC TATACCCTGC GCTGGTACCA TCGAGCTGTG 
CCTGGTGGCA CAGCCTGTCA TCCCTTTGTC TATGGTGGCT GCGGAGGGAA TGCCAATCGT 
TTTGGAACCC GTGAGGCGTG TGAGCGCCGC TGCCCACCCC AAGGGGTCCA CAGCCAGAAA 
ACAGGTGCTG CCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_031764.2
CDS	1..8835
Translation	MRLRLLVAALCAAEILMGAPEVWAQPRDRVTCTRLYAADIVFLLDGSSSIGRSNFREVRGFLEGLVLPFSGAASAQGVRFATVQYSDDPQTEFGLDTLGSGSDTIRAIRELSYKGGNTRTGAALHHVSDRVFLPRLTRPGVPKVCILITDGKSQDLVDTAAQKLKGQGVKLFAVGIKNADPEELKRVASQPTSDFFFFVNDFSILRTLLPLISRRVCTTAGGVPVTLPSDDTPSGPRDLVLSEPSSQSLRVQWTAASGPVTGYKVQYTPLTGLGQPLPSERQEVNIPAGETSTRLQGLRPLTDYQVTVVALYANSIGEAVSGTARTTAKEGLELSLQNITSHSLLVAWRRVPGANGYRVTWRDLSGGPTQQQDLSPGQGSVFLDHLEPGTDYEVTVSALFGHSVGPAASLTARTASSVEQTLHPIILSPTSILLSWNLVPEARGYRLEWRRESGLETPQKVELPPDVTRHQLDGLQPGTEYRLTLYTLLEGREVATPATVVPTGLEQLVSPVMNLQAIELPGQRVRVSWNPVPGATEYRFTVRTTQGVERTLLLPGSQTTFDLDDVRAGLSYTVRVSARVGAQEGDASILTIHRDPEAPLVVPGLRVVASDATRIRVAWGLVPGASGFRISWRTGSGPESSRTLTPDSTVTDILGLQPSTSYQVAVSALRGREEGPPVVIVARTDPLGPVRRVHLTQAGSSSVSITWTGVPGATGYRVSWHSGHGPEKSLLVSGDATVAEIDGLEPDTEYIVRVRTHVAGVDGAPASVVVRTAPEPVGSVSKLQILNASSDVLRVTWVGVPGATSYKLAWGRSEGGPMKHRILPGNKESAEIRDLEGGVSYSVRVTALVGDREGAPVSIVITTPPATPALLETLQVVQSGEHSLRLRWEPVPGAPGFRLHWQPEGGQEQSLTLGPESNSYNLVGLEPATKYQVWLTVLGQTGEGPPRKVTAYTEPSHIPSTELRVVDTSIDSVTLTWTPVSGASSYILSWRPLRGTGQEVPRAPQTLPGTSSSHRVTGLEPGISYVFSLTPIQSGVRGSEISVTQTPACSHGPVDVVFLLHATRDNAHNAEAVRRVLERLVSALGPLGPQAAQVGLLTYSHRPSPLFPLNSSHDLGIILRKIRDIPYVDPSGNNLGTAVTTAHRYLLASNAPGRRQQVPGVMVLLVDEPLRGDILSPIREAQTSGLKVMALSLVGADPEQLRRLAPGTDPIQNFFAVDNGPGLDRAVSDLAVALCQAAVTIEPQTGPCAVHCPKGQKGEPGVTGLQGQAGPPGPPGLPGRTGAPGPQGPPGSTQAKGERGFPGPEGPPGSPGLPGVPGSPGIKGSTGRPGPRGEQGERGPQGPKGEPGEPGQITGGGGPGFPGKKGDPGPSGPPGSRGPVGDPGPRGPPGLPGISVKGDKGDRGERGPPGPGIGASEQGDPGLPGLPGSPGPQGPAGRPGEKGEKGDCEDGGPGLPGQPGPPGEPGLRGAPGMTGPKGDRGLTGTPGEPGVKGERGHPGPVGPQGLPGAAGHPGVEGPEGPPGPTGRRGEKGEPGRPGDPAVGPGGAGAKGEKGEAGLPGPRGASGSKGEQGAPGLALPGDPGPKGDPGDRGPIGLTGRAGPTGDSGPPGEKGEPGRPGSPGPVGPRGRDGEAGEKGDEGIPGEPGLPGKAGERGLRGAPGPRGPVGEKGDQGDPGEDGRNGSPGSSGPKGDRGEPGPPGPPGRLVDAGIESRDKGEPGQEGPRGPKGDPGPPGVSGERGIDGLRGPPGPQGDPGVRGPAGDKGDRGPPGLDGRSGLDGKPGAPGPPGLHGASGKAGDPGRDGLPGLRGEHGPPGPPGPPGVPGKAGDDGKPGLNGKNGDPGDPGEDGRKGEKGDSGAPGREGPDGPKGERGAPGNPGLQGPPGLPGQVGPPGQGFPGVPGITGPKGDRGETGSKGEQGLPGERGLRGEPGSLPNAERLLETAGIKVSALREIVDTWDESSGSFLPVPERRPGPKGDPGDRGPPGKEGLIGFPGERGLKGERGDPGPQGPPGLALGERGPPGPPGLAGEPGKPGIPGLPGRAGGSGEAGRPGERGERGEKGERGDQGRDGLPGLPGPPGPPGPKVAIEEPGPGLAREQGPPGLKGAKGEPGSDGDPGPKGDRGVPGIKGDVGEPGKRGHDGNPGLPGERGVAGPEGKPGLQGPRGTPGPVGSHGDPGPPGAPGLAGPAGPQGPSGLKGEPGETGPPGRGLPGPVGAVGLPGPPGPSGLVGPQGSPGLPGQVGETGKPGPPGRDGSSGKDGDRGSPGVPGSPGLPGPVGPKGEPGPVGAPGQVVVGPPGAKGEKGAPGDLAGALLGEPGAKGDRGLPGPRGEKGEAGRAGGPGDPGEDGQKGAPGLKGLKGEPGIGVQGPPGPSGPPGMKGDLGPPGAPGAPGVVGFPGQTGPRGETGQPGPVGERGLAGPPGREGAPGPLGPPGPPGSAGAPGASGLKGDKGDPGAGLPGPRGERGEPGVRGEDGHPGQEGPRGLVGPPGSRGEQGEKGAAGAAGLKGDKGDSAVIEGPPGPRGAKGDMGERGPRGIDGDKGPRGESGNPGDKGSKGEPGDKGSAGSIGVRGLTGPKGEPGAAGIPGEPGAPGKDGIPGFRGDKGDIGFMGPRGLKGEKGIKGTCGRDGERGDKGEAGFPGRPGLAGKKGDMGEPGLPGQSGAPGKEGLIGPKGDRGFDGQSGPKGDQGEKGERGPPGVGGFPGPRGNDGSSGPPGPPGGVGPKGPEGLQGQKGERGPPGESVVGAPGAPGTPGERGEQGRPGPAGPRGEKGEAALTEDDIRDFVRQEMSQHCACQGQFIASGSRPLPGYAADTAGSQLHHVPVLRVSHVEEEGQVPPEDDDDFSEYSVYSVEDYQEPEVPWDGEAEIKGWDQRGSDLCSLPLDEGSCTAYTLRWYHRAVPGGTACHPFVYGGCGGNANRFGTREACERRCPPQGVHSQKTGAA

Target ORF information:

RefSeq Version	NM_007738.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus collagen, type VII, alpha 1 (Col7a1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_007738.3

ORF Insert Sequence:

ATGAGGCTGC GACTCCTGGT CGCTGCGCTC TGCGCTGCAG AGATCCTGAT GGGAGCGCCG 
GAAGTGTGGG CCCAGCCCAG AGATAGAGTG ACCTGCACAC GCCTTTATGC TGCTGACATC 
GTGTTCTTAC TCGATGGCTC GTCGTCCATT GGCCGCAGCA ACTTTCGAGA AGTGCGAGGA 
TTCCTGGAGG GGCTGGTGCT GCCTTTCTCA GGAGCAGCCA GTGCACAGGG CGTCCGGTTT 
GCCACAGTGC AGTACAGTGA TGATCCACAG ACAGAATTTG GTCTGGATAC ACTTGGCTCT 
GGGAGTGACA CCATCCGAGC CATCCGTGAG CTCAGCTATA AAGGTGGTAA CACTCGCACG 
GGGGCCGCTC TTCACCACGT TTCTGACCGT GTCTTCCTGC CTCGTCTAAC ACGTCCTGGT 
GTCCCCAAGG TCTGCATCCT GATCACAGAT GGGAAATCCC AAGATTTAGT GGACACAGCT 
GCCCAAAAGC TGAAGGGGCA GGGAGTCAAG CTGTTTGCTG TGGGAATCAA GAATGCCGAC 
CCTGAGGAAC TGAAGCGTGT GGCCTCACAG CCAACCAGCG ATTTCTTCTT CTTCGTCAAT 
GACTTCAGCA TCTTGAGGAC CCTGTTGCCT CTCATTTCCA GGAGGGTGTG CACAACTGCT 
GGTGGTGTGC CTGTGACCCT GCCTTCTGAT GACACTCCGT CTGGGCCCCG GGACCTGGTG 
CTCTCTGAGC CAAGTAGCCA ATCCTTGCGA GTACAATGGA CAGCAGCCAG TGGCCCTGTG 
ACTGGCTACA AGGTTCAGTA CACACCTCTG ACAGGGCTAG GGCAGCCACT GCCAAGTGAG 
CGACAGGAGG TGAACATCCC GGCTGGTGAG ACCAGCACGC GTCTCCAGGG TCTCCGGCCA 
CTAACGGACT ACCAAGTGAC TGTGGTTGCC CTCTATGCCA ACAGCATCGG GGAGGCCGTG 
AGTGGGACAG CTCGGACCAC TGCCAAGGAA GGGCTAGAAT TGAGCCTCCA GAACATCACA 
TCGCACAGCC TCCTGGTGGC CTGGAGGAGG GTACCAGGTG CTAATGGCTA CCGTGTAACA 
TGGCGGGACC TCAGTGGTGG GCCAACCCAG CAGCAGGACC TGAGCCCTGG ACAGGGGTCA 
GTGTTTCTAG ATCACTTGGA GCCTGGCACG GACTATGAGG TGACCGTGAG CGCCCTATTT 
GGCCACAGCG TGGGGCCTGC CGCTTCTCTG ACTGCCCGCA CCGCATCTTC TGTTGAGCAA 
ACCCTGCATC CCATCATCTT GAGCCCGACA TCCATCCTCC TTTCCTGGAA CTTGGTGCCT 
GAGGCCCGCG GCTACCGCTT GGAGTGGCGT CGTGAGAGTG GCTTGGAAAC ACCACAGAAA 
GTGGAACTGC CCCCTGATGT GACCCGCCAT CAGCTGGATG GTTTGCAGCC AGGCACTGAG 
TACCGTCTCA CGCTGTATAC TCTGCTGGAG GGCCGTGAGG TGGCCACTCC TGCAACCGTG 
GTTCCTACAG GGCTAGAGCA GCTGGTGAGC CCGGTGATGA ATTTACAAGC CATAGAACTA 
CCTGGGCAGC GGGTGCGAGT GTCTTGGAAC CCAGTCCCTG GGGCCACTGA GTACCGTTTC 
ACTGTACGCA CCACCCAGGG GGTCGAGCGG ACTCTATTGC TTCCTGGGAG CCAGACAACC 
TTTGACTTAG ATGATGTTCG AGCTGGGCTT AGCTACACGG TGCGGGTGTC TGCTCGAGTG 
GGTGCTCAGG AGGGTGATGC CAGCATCCTA ACGATCCACA GAGATCCAGA AGCCCCACTT 
GTTGTCCCAG GACTGCGGGT TGTCGCATCA GATGCAACAA GAATTAGGGT GGCCTGGGGA 
CTTGTCCCTG GAGCCAGTGG ATTTCGGATC AGCTGGAGGA CAGGCAGTGG TCCAGAGTCC 
AGCCGGACGC TGACCCCAGA CTCCACTGTC ACGGACATCC TGGGGTTGCA GCCTAGCACC 
TCTTATCAAG TCGCTGTCTC AGCACTTAGA GGGAGAGAAG AGGGGCCCCC TGTGGTCATC 
GTGGCTAGAA CTGATCCACT GGGCCCAGTG AGGAGAGTCC ACTTGACTCA GGCTGGCAGC 
TCATCTGTCA GCATCACCTG GACTGGGGTT CCCGGTGCCA CAGGATACAG GGTTTCCTGG 
CACTCAGGCC ATGGTCCAGA GAAATCTCTG TTGGTTTCTG GAGATGCCAC GGTGGCTGAA 
ATAGATGGGC TGGAGCCGGA CACAGAGTAT ATAGTGCGTG TGAGAACCCA CGTAGCTGGT 
GTGGATGGAG CCCCCGCCTC TGTGGTTGTG AGGACTGCTC CTGAGCCTGT GGGCAGTGTG 
TCGAAACTGC AAATCCTTAA TGCTTCCAGT GATGTTCTAC GGGTCACTTG GGTAGGAGTC 
CCTGGGGCCA CATCCTATAA ACTGGCGTGG GGACGTAGTG AAGGTGGCCC TATGAAACAC 
CGGATACTCC CAGGAAACAA AGAGTCTGCA GAGATACGGG ATCTCGAAGG AGGAGTCAGC 
TACTCCGTGA GAGTGACGGC GCTTGTTGGA GACCGAGAGG GTGCACCGGT GTCCATTGTC 
ATCACCACAC CGCCTGCGAC CCCAGCACTT CTGGAGACTC TTCAAGTTGT GCAGAGCGGG 
GAGCACTCCT TGAGGCTGCG ATGGGAACCA GTACCCGGAG CACCAGGCTT CCGCCTGCAC 
TGGCAACCTG AGGGTGGCCA GGAACAGTCC CTGACCTTGG GACCTGAGTC TAACAGCTAC 
AACCTGGTTG GTCTGGAGCC AGCAACAAAG TACCAAGTAT GGCTGACTGT CCTGGGCCAG 
ACTGGAGAAG GTCCCCCTAG GAAGGTGACT GCATACACTG AGCCCTCTCA CATCCCGAGC 
ACTGAACTAC GCGTGGTGGA TACCTCTATT GACTCAGTGA CTTTGACCTG GACCCCAGTG 
TCTGGGGCAT CCAGTTATAT CTTGTCCTGG CGGCCGCTCA GAGGAACTGG CCAGGAAGTG 
CCGAGGGCCC CACAGACACT GCCAGGGACC TCAAGCTCCC ACAGAGTGAC AGGGCTGGAA 
CCTGGTATTT CCTATGTTTT CTCTCTGACA CCTATCCAGA GCGGTGTACG AGGTTCTGAG 
ATCTCTGTCA CACAAACACC AGCGTGTTCC CATGGCCCAG TGGACGTGGT GTTCCTCTTG 
CATGCCACTC GAGACAATGC TCACAACGCA GAGGCTGTGA GGAGGGTCCT GGAAAGGCTG 
GTGTCTGCAC TTGGGCCTCT TGGTCCACAG GCTGCTCAGG TCGGCCTGCT GACTTACAGC 
CACCGGCCCT CCCCACTGTT CCCACTGAAT AGTTCCCATG ATCTTGGCAT CATCTTGCGA 
AAGATCCGTG ACATCCCCTA CGTGGACCCA AGTGGTAACA ACCTCGGCAC AGCCGTGACC 
ACAGCTCACA GATACCTCTT AGCATCCAAT GCTCCTGGTC GCCGTCAGCA AGTGCCAGGC 
GTGATGGTCC TGTTAGTGGA TGAGCCTCTG AGAGGGGACA TACTCAGCCC CATTCGTGAG 
GCCCAGACTT CTGGGCTTAA GGTGATGGCA CTGAGTTTGG TGGGAGCTGA CCCAGAGCAG 
CTGCGTCGCT TGGCACCAGG CACGGACCCC ATCCAGAACT TCTTTGCTGT GGATAATGGC 
CCAGGCTTGG ACAGAGCAGT CAGTGATCTG GCTGTAGCCC TGTGTCAGGC AGCTGTGACC 
ATAGAGCCGC AAACAGGGCC TTGTGCTGTG CACTGTCCAA AGGGCCAGAA GGGGGAGCCT 
GGAGTAACGG GACTGCAAGG ACAGGCTGGA CCTCCTGGCC CCCCTGGTCT CCCGGGCAGG 
ACCGGTGCTC CAGGCCCGCA AGGTCCTCCT GGAAGTACCC AGGCAAAAGG CGAGAGGGGC 
TTTCCTGGCC CAGAAGGGCC TCCAGGCAGT CCTGGCCTCC CTGGAGTTCC TGGGTCTCCT 
GGCATTAAGG GCTCCACGGG GCGGCCTGGC CCTCGTGGGG AACAGGGAGA ACGAGGGCCT 
CAAGGTCCAA AGGGGGAGCC GGGTGAGCCT GGGCAAATCA CTGGCGGTGG AGGGCCAGGG 
TTTCCTGGAA AGAAAGGGGA CCCTGGACCT TCGGGGCCCC CTGGATCTCG TGGCCCTGTG 
GGGGACCCAG GACCCCGTGG CCCCCCAGGG CTTCCTGGAA TATCAGTGAA GGGTGACAAA 
GGTGATCGTG GGGAACGGGG TCCCCCGGGA CCAGGCATTG GTGCCAGTGA ACAGGGTGAC 
CCTGGACTTC CGGGGCTTCC TGGAAGCCCT GGACCCCAAG GCCCAGCTGG CCGACCTGGA 
GAGAAGGGAG AAAAGGGTGA CTGTGAGGAT GGAGGTCCAG GCCTCCCAGG ACAGCCTGGG 
CCCCCAGGGG AGCCGGGCCT GCGGGGAGCT CCTGGAATGA CTGGGCCCAA AGGTGATCGG 
GGACTGACGG GGACCCCGGG CGAGCCTGGA GTAAAGGGTG AACGGGGACA TCCTGGTCCC 
GTGGGACCCC AGGGGCTCCC CGGGGCTGCT GGACACCCTG GAGTGGAGGG TCCTGAGGGG 
CCACCAGGAC CCACTGGCCG CCGAGGAGAA AAGGGAGAAC CTGGTCGCCC TGGGGATCCT 
GCAGTGGGTC CTGGTGGTGC TGGAGCCAAA GGAGAAAAGG GAGAGGCTGG GCTCCCTGGG 
CCCAGAGGAG CTTCTGGAAG CAAAGGAGAG CAGGGCGCAC CTGGATTGGC TCTTCCTGGA 
GATCCTGGCC CCAAAGGAGA CCCTGGAGAC AGGGGTCCCA TTGGCCTCAC TGGAAGGGCA 
GGACCCACAG GTGACTCAGG GCCTCCTGGA GAGAAAGGAG AGCCTGGTCG ACCTGGTTCC 
CCAGGACCTG TTGGCCCCCG AGGAAGAGAT GGTGAAGCTG GAGAGAAAGG TGACGAGGGG 
ATCCCGGGTG AGCCAGGTTT GCCTGGAAAA GCAGGTGAAC GTGGCCTCCG GGGGGCACCT 
GGGCCTCGGG GGCCTGTGGG TGAGAAGGGG GACCAGGGAG ATCCTGGAGA AGACGGACGG 
AATGGCAGCC CTGGATCATC TGGACCCAAG GGTGACCGTG GGGAGCCTGG TCCCCCAGGT 
CCCCCTGGAC GGCTGGTGGA TGCAGGCATT GAATCCAGAG ACAAGGGAGA GCCTGGACAA 
GAAGGTCCCC GAGGACCCAA GGGTGACCCT GGGCCCCCTG GAGTCTCTGG AGAAAGGGGC 
ATTGATGGGC TTCGGGGACC CCCAGGCCCA CAGGGAGACC CCGGTGTTCG AGGCCCAGCA 
GGAGACAAGG GTGATCGGGG ACCCCCAGGG CTGGATGGCC GGAGTGGGCT GGATGGGAAA 
CCCGGAGCCC CTGGCCCCCC AGGGCTACAT GGTGCTTCAG GCAAAGCTGG GGACCCGGGG 
AGAGATGGAC TTCCAGGCCT TCGAGGAGAA CATGGCCCCC CTGGTCCCCC TGGCCCTCCT 
GGAGTTCCGG GAAAGGCAGG CGATGATGGC AAACCAGGCC TGAATGGGAA AAACGGAGAC 
CCTGGAGACC CTGGAGAAGA TGGAAGGAAG GGAGAAAAGG GAGATTCGGG TGCCCCTGGA 
AGAGAAGGCC CTGATGGCCC CAAGGGTGAA CGTGGAGCTC CTGGCAATCC TGGACTCCAG 
GGTCCTCCGG GTCTCCCAGG GCAGGTCGGT CCTCCCGGCC AGGGTTTCCC TGGTGTCCCA 
GGAATTACGG GTCCTAAGGG TGACCGTGGA GAGACTGGAT CCAAAGGGGA ACAGGGCCTT 
CCTGGAGAAC GTGGCCTACG AGGAGAACCT GGGAGCTTGC CAAACGCGGA GCGTTTGCTG 
GAAACTGCTG GCATCAAGGT GTCGGCCCTG CGGGAGATCG TGGACACCTG GGATGAGAGT 
TCTGGCAGCT TCCTACCGGT GCCTGAGCGG AGACCTGGCC CCAAGGGGGA CCCTGGTGAC 
CGAGGCCCTC CAGGCAAGGA GGGCCTCATC GGCTTTCCTG GAGAACGTGG ACTGAAGGGA 
GAGCGTGGAG ACCCTGGCCC TCAGGGGCCT CCTGGCCTGG CCCTGGGTGA GAGGGGCCCA 
CCTGGCCCAC CCGGCCTTGC GGGGGAACCC GGAAAGCCTG GCATTCCTGG ACTCCCAGGC 
CGGGCTGGTG GTTCAGGGGA AGCAGGAAGG CCAGGAGAGA GGGGAGAAAG GGGAGAGAAA 
GGAGAACGCG GGGATCAGGG CAGAGATGGC CTTCCCGGCC TCCCTGGACC ACCTGGCCCT 
CCTGGCCCCA AGGTGGCCAT TGAAGAGCCA GGCCCTGGAC TGGCTAGAGA GCAAGGACCT 
CCTGGACTCA AGGGTGCCAA GGGGGAGCCA GGCAGTGATG GTGACCCTGG ACCTAAAGGA 
GACAGGGGTG TACCAGGCAT CAAAGGCGAT GTGGGAGAGC CTGGAAAGAG GGGTCATGAT 
GGCAACCCAG GTCTCCCGGG AGAGCGTGGT GTGGCTGGGC CTGAAGGGAA GCCGGGTCTT 
CAGGGCCCAA GGGGGACCCC TGGCCCAGTG GGTAGCCATG GAGACCCTGG ACCACCTGGT 
GCCCCGGGTC TTGCTGGCCC TGCGGGACCC CAGGGGCCTT CTGGCCTGAA GGGGGAGCCT 
GGAGAGACAG GACCTCCAGG ACGGGGTCTG CCTGGACCTG TTGGAGCTGT GGGACTCCCT 
GGCCCTCCTG GCCCTTCCGG CCTCGTGGGT CCACAAGGCT CTCCAGGTTT GCCCGGACAA 
GTGGGGGAGA CAGGGAAGCC AGGACCTCCA GGCCGTGACG GTAGCAGTGG AAAGGACGGA 
GATCGGGGAA GTCCTGGTGT GCCGGGGTCA CCAGGTCTGC CCGGCCCTGT TGGACCTAAA 
GGAGAACCTG GGCCTGTGGG GGCTCCTGGA CAGGTGGTGG TCGGGCCCCC TGGAGCAAAA 
GGCGAGAAGG GAGCCCCGGG AGACCTTGCT GGAGCCCTGC TGGGTGAGCC GGGAGCCAAA 
GGTGACCGAG GACTGCCTGG ACCACGGGGT GAAAAGGGTG AAGCTGGCCG TGCAGGAGGA 
CCTGGGGACC CTGGGGAAGA TGGTCAAAAA GGGGCTCCAG GACTCAAAGG TCTTAAGGGT 
GAGCCAGGCA TTGGGGTTCA GGGCCCCCCT GGCCCAAGTG GTCCTCCAGG TATGAAGGGA 
GACTTGGGCC CCCCTGGTGC CCCCGGGGCT CCTGGTGTTG TTGGGTTCCC TGGTCAGACA 
GGACCTCGAG GAGAGACGGG CCAGCCAGGG CCTGTTGGAG AGAGGGGTCT GGCAGGCCCT 
CCAGGGAGAG AAGGTGCCCC AGGTCCCTTG GGACCACCTG GACCACCAGG GTCAGCGGGA 
GCACCTGGCG CCTCTGGACT CAAAGGAGAC AAGGGAGACC CGGGAGCAGG ACTGCCGGGG 
CCCCGAGGTG AGCGTGGTGA GCCAGGTGTC CGGGGTGAAG ATGGCCACCC TGGCCAGGAA 
GGACCTCGTG GACTTGTGGG GCCCCCTGGC AGCCGGGGAG AGCAAGGAGA GAAGGGCGCT 
GCTGGCGCTG CAGGGCTCAA GGGTGACAAG GGTGACTCAG CTGTGATTGA GGGACCTCCC 
GGGCCACGAG GTGCCAAAGG GGATATGGGT GAACGAGGGC CTCGGGGCAT AGATGGTGAC 
AAAGGACCTC GGGGAGAAAG TGGGAATCCT GGTGACAAGG GTTCCAAAGG AGAGCCAGGT 
GACAAAGGCT CAGCTGGATC GATTGGAGTT CGAGGACTCA CAGGACCCAA GGGAGAGCCA 
GGTGCTGCAG GGATCCCCGG TGAGCCGGGA GCCCCAGGAA AGGATGGAAT CCCTGGTTTC 
CGAGGAGACA AAGGAGATAT TGGCTTCATG GGTCCCAGGG GCCTCAAGGG TGAAAAAGGA 
ATAAAGGGAA CCTGTGGCCG TGACGGAGAG AGAGGAGATA AGGGAGAAGC TGGTTTTCCT 
GGTCGCCCTG GGTTGGCAGG AAAGAAAGGA GACATGGGGG AGCCTGGTCT GCCGGGTCAG 
TCTGGGGCTC CTGGGAAAGA AGGCCTGATC GGTCCCAAGG GTGACAGAGG CTTTGATGGG 
CAGTCAGGAC CCAAGGGTGA CCAAGGCGAG AAAGGGGAGA GGGGGCCTCC AGGAGTTGGG 
GGCTTCCCAG GTCCCAGAGG AAACGATGGC TCTAGTGGTC CCCCAGGGCC ACCTGGTGGT 
GTTGGTCCCA AGGGACCCGA AGGGCTTCAG GGTCAGAAGG GTGAGCGAGG TCCCCCTGGA 
GAGAGCGTGG TGGGGGCTCC TGGTGCTCCT GGCACCCCTG GAGAGAGAGG AGAGCAGGGA 
CGACCAGGAC CTGCTGGCCC CCGTGGGGAG AAGGGAGAAG CTGCGCTGAC TGAGGATGAC 
ATTCGGGACT TTGTGCGTCA GGAGATGAGT CAGCACTGTG CCTGCCAGGG CCAGTTTATT 
GCGTCTGGAT CACGACCCCT CCCCGGTTAT GCTGCGGATA CAGCTGGTTC CCAGCTTCAC 
CATGTTCCTG TGCTCCGGGT CTCTCATGTT GAGGAGGAAG GCCAGGTGCC CCCTGAAGAT 
GACGATGACT TCTCTGAGTA CTCTGTGTAT TCTGTGGAGG ACTACCAGGA GCCTGAAGTT 
CCATGGGATG GTGAAGCCGA GATTAAGGGC TGGGACCAGA GAGGTTCAGA TCTCTGCTCC 
CTACCCTTGG ATGAGGGCTC CTGTACTGCC TATACCCTGC GCTGGTACCA TCGAGCTGTG 
CCTGGTGGCA CAGCCTGTCA TCCCTTTGTC TATGGTGGCT GCGGAGGGAA TGCCAATCGT 
TTTGGAACCC GTGAGGCGTG TGAGCGCCGC TGCCCACCCC AAGGGGTCCA CAGCCAGAAA 
ACAGGTGCTG CCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Col7a1 ( XM_006511935.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006511998.1 Mus musculus collagen, type VII, alpha 1 (Col7a1), transcript variant X1, mRNA.

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CloneID	OMu18740
Clone ID Related Accession (Same CDS sequence)	NM_007738.4 , XM_006511935.3 , NM_007738.3
Accession Version	XM_006511935.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 8835bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	collagen alpha-1(VII) chain isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000075.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006511935.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGCTGC GACTCCTGGT CGCTGCGCTC TGCGCTGCAG AGATCCTGAT GGGAGCGCCG 
GAAGTGTGGG CCCAGCCCAG AGATAGAGTG ACCTGCACAC GCCTTTATGC TGCTGACATC 
GTGTTCTTAC TCGATGGCTC GTCGTCCATT GGCCGCAGCA ACTTTCGAGA AGTGCGAGGA 
TTCCTGGAGG GGCTGGTGCT GCCTTTCTCA GGAGCAGCCA GTGCACAGGG CGTCCGGTTT 
GCCACAGTGC AGTACAGTGA TGATCCACAG ACAGAATTTG GTCTGGATAC ACTTGGCTCT 
GGGAGTGACA CCATCCGAGC CATCCGTGAG CTCAGCTATA AAGGTGGTAA CACTCGCACG 
GGGGCCGCTC TTCACCACGT TTCTGACCGT GTCTTCCTGC CTCGTCTAAC ACGTCCTGGT 
GTCCCCAAGG TCTGCATCCT GATCACAGAT GGGAAATCCC AAGATTTAGT GGACACAGCT 
GCCCAAAAGC TGAAGGGGCA GGGAGTCAAG CTGTTTGCTG TGGGAATCAA GAATGCCGAC 
CCTGAGGAAC TGAAGCGTGT GGCCTCACAG CCAACCAGCG ATTTCTTCTT CTTCGTCAAT 
GACTTCAGCA TCTTGAGGAC CCTGTTGCCT CTCATTTCCA GGAGGGTGTG CACAACTGCT 
GGTGGTGTGC CTGTGACCCT GCCTTCTGAT GACACTCCGT CTGGGCCCCG GGACCTGGTG 
CTCTCTGAGC CAAGTAGCCA ATCCTTGCGA GTACAATGGA CAGCAGCCAG TGGCCCTGTG 
ACTGGCTACA AGGTTCAGTA CACACCTCTG ACAGGGCTAG GGCAGCCACT GCCAAGTGAG 
CGACAGGAGG TGAACATCCC GGCTGGTGAG ACCAGCACGC GTCTCCAGGG TCTCCGGCCA 
CTAACGGACT ACCAAGTGAC TGTGGTTGCC CTCTATGCCA ACAGCATCGG GGAGGCCGTG 
AGTGGGACAG CTCGGACCAC TGCCAAGGAA GGGCTAGAAT TGAGCCTCCA GAACATCACA 
TCGCACAGCC TCCTGGTGGC CTGGAGGAGG GTACCAGGTG CTAATGGCTA CCGTGTAACA 
TGGCGGGACC TCAGTGGTGG GCCAACCCAG CAGCAGGACC TGAGCCCTGG ACAGGGGTCA 
GTGTTTCTAG ATCACTTGGA GCCTGGCACG GACTATGAGG TGACCGTGAG CGCCCTATTT 
GGCCACAGCG TGGGGCCTGC CGCTTCTCTG ACTGCCCGCA CCGCATCTTC TGTTGAGCAA 
ACCCTGCATC CCATCATCTT GAGCCCGACA TCCATCCTCC TTTCCTGGAA CTTGGTGCCT 
GAGGCCCGCG GCTACCGCTT GGAGTGGCGT CGTGAGAGTG GCTTGGAAAC ACCACAGAAA 
GTGGAACTGC CCCCTGATGT GACCCGCCAT CAGCTGGATG GTTTGCAGCC AGGCACTGAG 
TACCGTCTCA CGCTGTATAC TCTGCTGGAG GGCCGTGAGG TGGCCACTCC TGCAACCGTG 
GTTCCTACAG GGCTAGAGCA GCTGGTGAGC CCGGTGATGA ATTTACAAGC CATAGAACTA 
CCTGGGCAGC GGGTGCGAGT GTCTTGGAAC CCAGTCCCTG GGGCCACTGA GTACCGTTTC 
ACTGTACGCA CCACCCAGGG GGTCGAGCGG ACTCTATTGC TTCCTGGGAG CCAGACAACC 
TTTGACTTAG ATGATGTTCG AGCTGGGCTT AGCTACACGG TGCGGGTGTC TGCTCGAGTG 
GGTGCTCAGG AGGGTGATGC CAGCATCCTA ACGATCCACA GAGATCCAGA AGCCCCACTT 
GTTGTCCCAG GACTGCGGGT TGTCGCATCA GATGCAACAA GAATTAGGGT GGCCTGGGGA 
CTTGTCCCTG GAGCCAGTGG ATTTCGGATC AGCTGGAGGA CAGGCAGTGG TCCAGAGTCC 
AGCCGGACGC TGACCCCAGA CTCCACTGTC ACGGACATCC TGGGGTTGCA GCCTAGCACC 
TCTTATCAAG TCGCTGTCTC AGCACTTAGA GGGAGAGAAG AGGGGCCCCC TGTGGTCATC 
GTGGCTAGAA CTGATCCACT GGGCCCAGTG AGGAGAGTCC ACTTGACTCA GGCTGGCAGC 
TCATCTGTCA GCATCACCTG GACTGGGGTT CCCGGTGCCA CAGGATACAG GGTTTCCTGG 
CACTCAGGCC ATGGTCCAGA GAAATCTCTG TTGGTTTCTG GAGATGCCAC GGTGGCTGAA 
ATAGATGGGC TGGAGCCGGA CACAGAGTAT ATAGTGCGTG TGAGAACCCA CGTAGCTGGT 
GTGGATGGAG CCCCCGCCTC TGTGGTTGTG AGGACTGCTC CTGAGCCTGT GGGCAGTGTG 
TCGAAACTGC AAATCCTTAA TGCTTCCAGT GATGTTCTAC GGGTCACTTG GGTAGGAGTC 
CCTGGGGCCA CATCCTATAA ACTGGCGTGG GGACGTAGTG AAGGTGGCCC TATGAAACAC 
CGGATACTCC CAGGAAACAA AGAGTCTGCA GAGATACGGG ATCTCGAAGG AGGAGTCAGC 
TACTCCGTGA GAGTGACGGC GCTTGTTGGA GACCGAGAGG GTGCACCGGT GTCCATTGTC 
ATCACCACAC CGCCTGCGAC CCCAGCACTT CTGGAGACTC TTCAAGTTGT GCAGAGCGGG 
GAGCACTCCT TGAGGCTGCG ATGGGAACCA GTACCCGGAG CACCAGGCTT CCGCCTGCAC 
TGGCAACCTG AGGGTGGCCA GGAACAGTCC CTGACCTTGG GACCTGAGTC TAACAGCTAC 
AACCTGGTTG GTCTGGAGCC AGCAACAAAG TACCAAGTAT GGCTGACTGT CCTGGGCCAG 
ACTGGAGAAG GTCCCCCTAG GAAGGTGACT GCATACACTG AGCCCTCTCA CATCCCGAGC 
ACTGAACTAC GCGTGGTGGA TACCTCTATT GACTCAGTGA CTTTGACCTG GACCCCAGTG 
TCTGGGGCAT CCAGTTATAT CTTGTCCTGG CGGCCGCTCA GAGGAACTGG CCAGGAAGTG 
CCGAGGGCCC CACAGACACT GCCAGGGACC TCAAGCTCCC ACAGAGTGAC AGGGCTGGAA 
CCTGGTATTT CCTATGTTTT CTCTCTGACA CCTATCCAGA GCGGTGTACG AGGTTCTGAG 
ATCTCTGTCA CACAAACACC AGCGTGTTCC CATGGCCCAG TGGACGTGGT GTTCCTCTTG 
CATGCCACTC GAGACAATGC TCACAACGCA GAGGCTGTGA GGAGGGTCCT GGAAAGGCTG 
GTGTCTGCAC TTGGGCCTCT TGGTCCACAG GCTGCTCAGG TCGGCCTGCT GACTTACAGC 
CACCGGCCCT CCCCACTGTT CCCACTGAAT AGTTCCCATG ATCTTGGCAT CATCTTGCGA 
AAGATCCGTG ACATCCCCTA CGTGGACCCA AGTGGTAACA ACCTCGGCAC AGCCGTGACC 
ACAGCTCACA GATACCTCTT AGCATCCAAT GCTCCTGGTC GCCGTCAGCA AGTGCCAGGC 
GTGATGGTCC TGTTAGTGGA TGAGCCTCTG AGAGGGGACA TACTCAGCCC CATTCGTGAG 
GCCCAGACTT CTGGGCTTAA GGTGATGGCA CTGAGTTTGG TGGGAGCTGA CCCAGAGCAG 
CTGCGTCGCT TGGCACCAGG CACGGACCCC ATCCAGAACT TCTTTGCTGT GGATAATGGC 
CCAGGCTTGG ACAGAGCAGT CAGTGATCTG GCTGTAGCCC TGTGTCAGGC AGCTGTGACC 
ATAGAGCCGC AAACAGGGCC TTGTGCTGTG CACTGTCCAA AGGGCCAGAA GGGGGAGCCT 
GGAGTAACGG GACTGCAAGG ACAGGCTGGA CCTCCTGGCC CCCCTGGTCT CCCGGGCAGG 
ACCGGTGCTC CAGGCCCGCA AGGTCCTCCT GGAAGTACCC AGGCAAAAGG CGAGAGGGGC 
TTTCCTGGCC CAGAAGGGCC TCCAGGCAGT CCTGGCCTCC CTGGAGTTCC TGGGTCTCCT 
GGCATTAAGG GCTCCACGGG GCGGCCTGGC CCTCGTGGGG AACAGGGAGA ACGAGGGCCT 
CAAGGTCCAA AGGGGGAGCC GGGTGAGCCT GGGCAAATCA CTGGCGGTGG AGGGCCAGGG 
TTTCCTGGAA AGAAAGGGGA CCCTGGACCT TCGGGGCCCC CTGGATCTCG TGGCCCTGTG 
GGGGACCCAG GACCCCGTGG CCCCCCAGGG CTTCCTGGAA TATCAGTGAA GGGTGACAAA 
GGTGATCGTG GGGAACGGGG TCCCCCGGGA CCAGGCATTG GTGCCAGTGA ACAGGGTGAC 
CCTGGACTTC CGGGGCTTCC TGGAAGCCCT GGACCCCAAG GCCCAGCTGG CCGACCTGGA 
GAGAAGGGAG AAAAGGGTGA CTGTGAGGAT GGAGGTCCAG GCCTCCCAGG ACAGCCTGGG 
CCCCCAGGGG AGCCGGGCCT GCGGGGAGCT CCTGGAATGA CTGGGCCCAA AGGTGATCGG 
GGACTGACGG GGACCCCGGG CGAGCCTGGA GTAAAGGGTG AACGGGGACA TCCTGGTCCC 
GTGGGACCCC AGGGGCTCCC CGGGGCTGCT GGACACCCTG GAGTGGAGGG TCCTGAGGGG 
CCACCAGGAC CCACTGGCCG CCGAGGAGAA AAGGGAGAAC CTGGTCGCCC TGGGGATCCT 
GCAGTGGGTC CTGGTGGTGC TGGAGCCAAA GGAGAAAAGG GAGAGGCTGG GCTCCCTGGG 
CCCAGAGGAG CTTCTGGAAG CAAAGGAGAG CAGGGCGCAC CTGGATTGGC TCTTCCTGGA 
GATCCTGGCC CCAAAGGAGA CCCTGGAGAC AGGGGTCCCA TTGGCCTCAC TGGAAGGGCA 
GGACCCACAG GTGACTCAGG GCCTCCTGGA GAGAAAGGAG AGCCTGGTCG ACCTGGTTCC 
CCAGGACCTG TTGGCCCCCG AGGAAGAGAT GGTGAAGCTG GAGAGAAAGG TGACGAGGGG 
ATCCCGGGTG AGCCAGGTTT GCCTGGAAAA GCAGGTGAAC GTGGCCTCCG GGGGGCACCT 
GGGCCTCGGG GGCCTGTGGG TGAGAAGGGG GACCAGGGAG ATCCTGGAGA AGACGGACGG 
AATGGCAGCC CTGGATCATC TGGACCCAAG GGTGACCGTG GGGAGCCTGG TCCCCCAGGT 
CCCCCTGGAC GGCTGGTGGA TGCAGGCATT GAATCCAGAG ACAAGGGAGA GCCTGGACAA 
GAAGGTCCCC GAGGACCCAA GGGTGACCCT GGGCCCCCTG GAGTCTCTGG AGAAAGGGGC 
ATTGATGGGC TTCGGGGACC CCCAGGCCCA CAGGGAGACC CCGGTGTTCG AGGCCCAGCA 
GGAGACAAGG GTGATCGGGG ACCCCCAGGG CTGGATGGCC GGAGTGGGCT GGATGGGAAA 
CCCGGAGCCC CTGGCCCCCC AGGGCTACAT GGTGCTTCAG GCAAAGCTGG GGACCCGGGG 
AGAGATGGAC TTCCAGGCCT TCGAGGAGAA CATGGCCCCC CTGGTCCCCC TGGCCCTCCT 
GGAGTTCCGG GAAAGGCAGG CGATGATGGC AAACCAGGCC TGAATGGGAA AAACGGAGAC 
CCTGGAGACC CTGGAGAAGA TGGAAGGAAG GGAGAAAAGG GAGATTCGGG TGCCCCTGGA 
AGAGAAGGCC CTGATGGCCC CAAGGGTGAA CGTGGAGCTC CTGGCAATCC TGGACTCCAG 
GGTCCTCCGG GTCTCCCAGG GCAGGTCGGT CCTCCCGGCC AGGGTTTCCC TGGTGTCCCA 
GGAATTACGG GTCCTAAGGG TGACCGTGGA GAGACTGGAT CCAAAGGGGA ACAGGGCCTT 
CCTGGAGAAC GTGGCCTACG AGGAGAACCT GGGAGCTTGC CAAACGCGGA GCGTTTGCTG 
GAAACTGCTG GCATCAAGGT GTCGGCCCTG CGGGAGATCG TGGACACCTG GGATGAGAGT 
TCTGGCAGCT TCCTACCGGT GCCTGAGCGG AGACCTGGCC CCAAGGGGGA CCCTGGTGAC 
CGAGGCCCTC CAGGCAAGGA GGGCCTCATC GGCTTTCCTG GAGAACGTGG ACTGAAGGGA 
GAGCGTGGAG ACCCTGGCCC TCAGGGGCCT CCTGGCCTGG CCCTGGGTGA GAGGGGCCCA 
CCTGGCCCAC CCGGCCTTGC GGGGGAACCC GGAAAGCCTG GCATTCCTGG ACTCCCAGGC 
CGGGCTGGTG GTTCAGGGGA AGCAGGAAGG CCAGGAGAGA GGGGAGAAAG GGGAGAGAAA 
GGAGAACGCG GGGATCAGGG CAGAGATGGC CTTCCCGGCC TCCCTGGACC ACCTGGCCCT 
CCTGGCCCCA AGGTGGCCAT TGAAGAGCCA GGCCCTGGAC TGGCTAGAGA GCAAGGACCT 
CCTGGACTCA AGGGTGCCAA GGGGGAGCCA GGCAGTGATG GTGACCCTGG ACCTAAAGGA 
GACAGGGGTG TACCAGGCAT CAAAGGCGAT GTGGGAGAGC CTGGAAAGAG GGGTCATGAT 
GGCAACCCAG GTCTCCCGGG AGAGCGTGGT GTGGCTGGGC CTGAAGGGAA GCCGGGTCTT 
CAGGGCCCAA GGGGGACCCC TGGCCCAGTG GGTAGCCATG GAGACCCTGG ACCACCTGGT 
GCCCCGGGTC TTGCTGGCCC TGCGGGACCC CAGGGGCCTT CTGGCCTGAA GGGGGAGCCT 
GGAGAGACAG GACCTCCAGG ACGGGGTCTG CCTGGACCTG TTGGAGCTGT GGGACTCCCT 
GGCCCTCCTG GCCCTTCCGG CCTCGTGGGT CCACAAGGCT CTCCAGGTTT GCCCGGACAA 
GTGGGGGAGA CAGGGAAGCC AGGACCTCCA GGCCGTGACG GTAGCAGTGG AAAGGACGGA 
GATCGGGGAA GTCCTGGTGT GCCGGGGTCA CCAGGTCTGC CCGGCCCTGT TGGACCTAAA 
GGAGAACCTG GGCCTGTGGG GGCTCCTGGA CAGGTGGTGG TCGGGCCCCC TGGAGCAAAA 
GGCGAGAAGG GAGCCCCGGG AGACCTTGCT GGAGCCCTGC TGGGTGAGCC GGGAGCCAAA 
GGTGACCGAG GACTGCCTGG ACCACGGGGT GAAAAGGGTG AAGCTGGCCG TGCAGGAGGA 
CCTGGGGACC CTGGGGAAGA TGGTCAAAAA GGGGCTCCAG GACTCAAAGG TCTTAAGGGT 
GAGCCAGGCA TTGGGGTTCA GGGCCCCCCT GGCCCAAGTG GTCCTCCAGG TATGAAGGGA 
GACTTGGGCC CCCCTGGTGC CCCCGGGGCT CCTGGTGTTG TTGGGTTCCC TGGTCAGACA 
GGACCTCGAG GAGAGACGGG CCAGCCAGGG CCTGTTGGAG AGAGGGGTCT GGCAGGCCCT 
CCAGGGAGAG AAGGTGCCCC AGGTCCCTTG GGACCACCTG GACCACCAGG GTCAGCGGGA 
GCACCTGGCG CCTCTGGACT CAAAGGAGAC AAGGGAGACC CGGGAGCAGG ACTGCCGGGG 
CCCCGAGGTG AGCGTGGTGA GCCAGGTGTC CGGGGTGAAG ATGGCCACCC TGGCCAGGAA 
GGACCTCGTG GACTTGTGGG GCCCCCTGGC AGCCGGGGAG AGCAAGGAGA GAAGGGCGCT 
GCTGGCGCTG CAGGGCTCAA GGGTGACAAG GGTGACTCAG CTGTGATTGA GGGACCTCCC 
GGGCCACGAG GTGCCAAAGG GGATATGGGT GAACGAGGGC CTCGGGGCAT AGATGGTGAC 
AAAGGACCTC GGGGAGAAAG TGGGAATCCT GGTGACAAGG GTTCCAAAGG AGAGCCAGGT 
GACAAAGGCT CAGCTGGATC GATTGGAGTT CGAGGACTCA CAGGACCCAA GGGAGAGCCA 
GGTGCTGCAG GGATCCCCGG TGAGCCGGGA GCCCCAGGAA AGGATGGAAT CCCTGGTTTC 
CGAGGAGACA AAGGAGATAT TGGCTTCATG GGTCCCAGGG GCCTCAAGGG TGAAAAAGGA 
ATAAAGGGAA CCTGTGGCCG TGACGGAGAG AGAGGAGATA AGGGAGAAGC TGGTTTTCCT 
GGTCGCCCTG GGTTGGCAGG AAAGAAAGGA GACATGGGGG AGCCTGGTCT GCCGGGTCAG 
TCTGGGGCTC CTGGGAAAGA AGGCCTGATC GGTCCCAAGG GTGACAGAGG CTTTGATGGG 
CAGTCAGGAC CCAAGGGTGA CCAAGGCGAG AAAGGGGAGA GGGGGCCTCC AGGAGTTGGG 
GGCTTCCCAG GTCCCAGAGG AAACGATGGC TCTAGTGGTC CCCCAGGGCC ACCTGGTGGT 
GTTGGTCCCA AGGGACCCGA AGGGCTTCAG GGTCAGAAGG GTGAGCGAGG TCCCCCTGGA 
GAGAGCGTGG TGGGGGCTCC TGGTGCTCCT GGCACCCCTG GAGAGAGAGG AGAGCAGGGA 
CGACCAGGAC CTGCTGGCCC CCGTGGGGAG AAGGGAGAAG CTGCGCTGAC TGAGGATGAC 
ATTCGGGACT TTGTGCGTCA GGAGATGAGT CAGCACTGTG CCTGCCAGGG CCAGTTTATT 
GCGTCTGGAT CACGACCCCT CCCCGGTTAT GCTGCGGATA CAGCTGGTTC CCAGCTTCAC 
CATGTTCCTG TGCTCCGGGT CTCTCATGTT GAGGAGGAAG GCCAGGTGCC CCCTGAAGAT 
GACGATGACT TCTCTGAGTA CTCTGTGTAT TCTGTGGAGG ACTACCAGGA GCCTGAAGTT 
CCATGGGATG GTGAAGCCGA GATTAAGGGC TGGGACCAGA GAGGTTCAGA TCTCTGCTCC 
CTACCCTTGG ATGAGGGCTC CTGTACTGCC TATACCCTGC GCTGGTACCA TCGAGCTGTG 
CCTGGTGGCA CAGCCTGTCA TCCCTTTGTC TATGGTGGCT GCGGAGGGAA TGCCAATCGT 
TTTGGAACCC GTGAGGCGTG TGAGCGCCGC TGCCCACCCC AAGGGGTCCA CAGCCAGAAA 
ACAGGTGCTG CCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006511998.1
CDS	64..8898
Translation	MRLRLLVAALCAAEILMGAPEVWAQPRDRVTCTRLYAADIVFLLDGSSSIGRSNFREVRGFLEGLVLPFSGAASAQGVRFATVQYSDDPQTEFGLDTLGSGSDTIRAIRELSYKGGNTRTGAALHHVSDRVFLPRLTRPGVPKVCILITDGKSQDLVDTAAQKLKGQGVKLFAVGIKNADPEELKRVASQPTSDFFFFVNDFSILRTLLPLISRRVCTTAGGVPVTLPSDDTPSGPRDLVLSEPSSQSLRVQWTAASGPVTGYKVQYTPLTGLGQPLPSERQEVNIPAGETSTRLQGLRPLTDYQVTVVALYANSIGEAVSGTARTTAKEGLELSLQNITSHSLLVAWRRVPGANGYRVTWRDLSGGPTQQQDLSPGQGSVFLDHLEPGTDYEVTVSALFGHSVGPAASLTARTASSVEQTLHPIILSPTSILLSWNLVPEARGYRLEWRRESGLETPQKVELPPDVTRHQLDGLQPGTEYRLTLYTLLEGREVATPATVVPTGLEQLVSPVMNLQAIELPGQRVRVSWNPVPGATEYRFTVRTTQGVERTLLLPGSQTTFDLDDVRAGLSYTVRVSARVGAQEGDASILTIHRDPEAPLVVPGLRVVASDATRIRVAWGLVPGASGFRISWRTGSGPESSRTLTPDSTVTDILGLQPSTSYQVAVSALRGREEGPPVVIVARTDPLGPVRRVHLTQAGSSSVSITWTGVPGATGYRVSWHSGHGPEKSLLVSGDATVAEIDGLEPDTEYIVRVRTHVAGVDGAPASVVVRTAPEPVGSVSKLQILNASSDVLRVTWVGVPGATSYKLAWGRSEGGPMKHRILPGNKESAEIRDLEGGVSYSVRVTALVGDREGAPVSIVITTPPATPALLETLQVVQSGEHSLRLRWEPVPGAPGFRLHWQPEGGQEQSLTLGPESNSYNLVGLEPATKYQVWLTVLGQTGEGPPRKVTAYTEPSHIPSTELRVVDTSIDSVTLTWTPVSGASSYILSWRPLRGTGQEVPRAPQTLPGTSSSHRVTGLEPGISYVFSLTPIQSGVRGSEISVTQTPACSHGPVDVVFLLHATRDNAHNAEAVRRVLERLVSALGPLGPQAAQVGLLTYSHRPSPLFPLNSSHDLGIILRKIRDIPYVDPSGNNLGTAVTTAHRYLLASNAPGRRQQVPGVMVLLVDEPLRGDILSPIREAQTSGLKVMALSLVGADPEQLRRLAPGTDPIQNFFAVDNGPGLDRAVSDLAVALCQAAVTIEPQTGPCAVHCPKGQKGEPGVTGLQGQAGPPGPPGLPGRTGAPGPQGPPGSTQAKGERGFPGPEGPPGSPGLPGVPGSPGIKGSTGRPGPRGEQGERGPQGPKGEPGEPGQITGGGGPGFPGKKGDPGPSGPPGSRGPVGDPGPRGPPGLPGISVKGDKGDRGERGPPGPGIGASEQGDPGLPGLPGSPGPQGPAGRPGEKGEKGDCEDGGPGLPGQPGPPGEPGLRGAPGMTGPKGDRGLTGTPGEPGVKGERGHPGPVGPQGLPGAAGHPGVEGPEGPPGPTGRRGEKGEPGRPGDPAVGPGGAGAKGEKGEAGLPGPRGASGSKGEQGAPGLALPGDPGPKGDPGDRGPIGLTGRAGPTGDSGPPGEKGEPGRPGSPGPVGPRGRDGEAGEKGDEGIPGEPGLPGKAGERGLRGAPGPRGPVGEKGDQGDPGEDGRNGSPGSSGPKGDRGEPGPPGPPGRLVDAGIESRDKGEPGQEGPRGPKGDPGPPGVSGERGIDGLRGPPGPQGDPGVRGPAGDKGDRGPPGLDGRSGLDGKPGAPGPPGLHGASGKAGDPGRDGLPGLRGEHGPPGPPGPPGVPGKAGDDGKPGLNGKNGDPGDPGEDGRKGEKGDSGAPGREGPDGPKGERGAPGNPGLQGPPGLPGQVGPPGQGFPGVPGITGPKGDRGETGSKGEQGLPGERGLRGEPGSLPNAERLLETAGIKVSALREIVDTWDESSGSFLPVPERRPGPKGDPGDRGPPGKEGLIGFPGERGLKGERGDPGPQGPPGLALGERGPPGPPGLAGEPGKPGIPGLPGRAGGSGEAGRPGERGERGEKGERGDQGRDGLPGLPGPPGPPGPKVAIEEPGPGLAREQGPPGLKGAKGEPGSDGDPGPKGDRGVPGIKGDVGEPGKRGHDGNPGLPGERGVAGPEGKPGLQGPRGTPGPVGSHGDPGPPGAPGLAGPAGPQGPSGLKGEPGETGPPGRGLPGPVGAVGLPGPPGPSGLVGPQGSPGLPGQVGETGKPGPPGRDGSSGKDGDRGSPGVPGSPGLPGPVGPKGEPGPVGAPGQVVVGPPGAKGEKGAPGDLAGALLGEPGAKGDRGLPGPRGEKGEAGRAGGPGDPGEDGQKGAPGLKGLKGEPGIGVQGPPGPSGPPGMKGDLGPPGAPGAPGVVGFPGQTGPRGETGQPGPVGERGLAGPPGREGAPGPLGPPGPPGSAGAPGASGLKGDKGDPGAGLPGPRGERGEPGVRGEDGHPGQEGPRGLVGPPGSRGEQGEKGAAGAAGLKGDKGDSAVIEGPPGPRGAKGDMGERGPRGIDGDKGPRGESGNPGDKGSKGEPGDKGSAGSIGVRGLTGPKGEPGAAGIPGEPGAPGKDGIPGFRGDKGDIGFMGPRGLKGEKGIKGTCGRDGERGDKGEAGFPGRPGLAGKKGDMGEPGLPGQSGAPGKEGLIGPKGDRGFDGQSGPKGDQGEKGERGPPGVGGFPGPRGNDGSSGPPGPPGGVGPKGPEGLQGQKGERGPPGESVVGAPGAPGTPGERGEQGRPGPAGPRGEKGEAALTEDDIRDFVRQEMSQHCACQGQFIASGSRPLPGYAADTAGSQLHHVPVLRVSHVEEEGQVPPEDDDDFSEYSVYSVEDYQEPEVPWDGEAEIKGWDQRGSDLCSLPLDEGSCTAYTLRWYHRAVPGGTACHPFVYGGCGGNANRFGTREACERRCPPQGVHSQKTGAA

Target ORF information:

RefSeq Version	XM_006511935.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus collagen, type VII, alpha 1 (Col7a1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006511935.3

ORF Insert Sequence:

ATGAGGCTGC GACTCCTGGT CGCTGCGCTC TGCGCTGCAG AGATCCTGAT GGGAGCGCCG 
GAAGTGTGGG CCCAGCCCAG AGATAGAGTG ACCTGCACAC GCCTTTATGC TGCTGACATC 
GTGTTCTTAC TCGATGGCTC GTCGTCCATT GGCCGCAGCA ACTTTCGAGA AGTGCGAGGA 
TTCCTGGAGG GGCTGGTGCT GCCTTTCTCA GGAGCAGCCA GTGCACAGGG CGTCCGGTTT 
GCCACAGTGC AGTACAGTGA TGATCCACAG ACAGAATTTG GTCTGGATAC ACTTGGCTCT 
GGGAGTGACA CCATCCGAGC CATCCGTGAG CTCAGCTATA AAGGTGGTAA CACTCGCACG 
GGGGCCGCTC TTCACCACGT TTCTGACCGT GTCTTCCTGC CTCGTCTAAC ACGTCCTGGT 
GTCCCCAAGG TCTGCATCCT GATCACAGAT GGGAAATCCC AAGATTTAGT GGACACAGCT 
GCCCAAAAGC TGAAGGGGCA GGGAGTCAAG CTGTTTGCTG TGGGAATCAA GAATGCCGAC 
CCTGAGGAAC TGAAGCGTGT GGCCTCACAG CCAACCAGCG ATTTCTTCTT CTTCGTCAAT 
GACTTCAGCA TCTTGAGGAC CCTGTTGCCT CTCATTTCCA GGAGGGTGTG CACAACTGCT 
GGTGGTGTGC CTGTGACCCT GCCTTCTGAT GACACTCCGT CTGGGCCCCG GGACCTGGTG 
CTCTCTGAGC CAAGTAGCCA ATCCTTGCGA GTACAATGGA CAGCAGCCAG TGGCCCTGTG 
ACTGGCTACA AGGTTCAGTA CACACCTCTG ACAGGGCTAG GGCAGCCACT GCCAAGTGAG 
CGACAGGAGG TGAACATCCC GGCTGGTGAG ACCAGCACGC GTCTCCAGGG TCTCCGGCCA 
CTAACGGACT ACCAAGTGAC TGTGGTTGCC CTCTATGCCA ACAGCATCGG GGAGGCCGTG 
AGTGGGACAG CTCGGACCAC TGCCAAGGAA GGGCTAGAAT TGAGCCTCCA GAACATCACA 
TCGCACAGCC TCCTGGTGGC CTGGAGGAGG GTACCAGGTG CTAATGGCTA CCGTGTAACA 
TGGCGGGACC TCAGTGGTGG GCCAACCCAG CAGCAGGACC TGAGCCCTGG ACAGGGGTCA 
GTGTTTCTAG ATCACTTGGA GCCTGGCACG GACTATGAGG TGACCGTGAG CGCCCTATTT 
GGCCACAGCG TGGGGCCTGC CGCTTCTCTG ACTGCCCGCA CCGCATCTTC TGTTGAGCAA 
ACCCTGCATC CCATCATCTT GAGCCCGACA TCCATCCTCC TTTCCTGGAA CTTGGTGCCT 
GAGGCCCGCG GCTACCGCTT GGAGTGGCGT CGTGAGAGTG GCTTGGAAAC ACCACAGAAA 
GTGGAACTGC CCCCTGATGT GACCCGCCAT CAGCTGGATG GTTTGCAGCC AGGCACTGAG 
TACCGTCTCA CGCTGTATAC TCTGCTGGAG GGCCGTGAGG TGGCCACTCC TGCAACCGTG 
GTTCCTACAG GGCTAGAGCA GCTGGTGAGC CCGGTGATGA ATTTACAAGC CATAGAACTA 
CCTGGGCAGC GGGTGCGAGT GTCTTGGAAC CCAGTCCCTG GGGCCACTGA GTACCGTTTC 
ACTGTACGCA CCACCCAGGG GGTCGAGCGG ACTCTATTGC TTCCTGGGAG CCAGACAACC 
TTTGACTTAG ATGATGTTCG AGCTGGGCTT AGCTACACGG TGCGGGTGTC TGCTCGAGTG 
GGTGCTCAGG AGGGTGATGC CAGCATCCTA ACGATCCACA GAGATCCAGA AGCCCCACTT 
GTTGTCCCAG GACTGCGGGT TGTCGCATCA GATGCAACAA GAATTAGGGT GGCCTGGGGA 
CTTGTCCCTG GAGCCAGTGG ATTTCGGATC AGCTGGAGGA CAGGCAGTGG TCCAGAGTCC 
AGCCGGACGC TGACCCCAGA CTCCACTGTC ACGGACATCC TGGGGTTGCA GCCTAGCACC 
TCTTATCAAG TCGCTGTCTC AGCACTTAGA GGGAGAGAAG AGGGGCCCCC TGTGGTCATC 
GTGGCTAGAA CTGATCCACT GGGCCCAGTG AGGAGAGTCC ACTTGACTCA GGCTGGCAGC 
TCATCTGTCA GCATCACCTG GACTGGGGTT CCCGGTGCCA CAGGATACAG GGTTTCCTGG 
CACTCAGGCC ATGGTCCAGA GAAATCTCTG TTGGTTTCTG GAGATGCCAC GGTGGCTGAA 
ATAGATGGGC TGGAGCCGGA CACAGAGTAT ATAGTGCGTG TGAGAACCCA CGTAGCTGGT 
GTGGATGGAG CCCCCGCCTC TGTGGTTGTG AGGACTGCTC CTGAGCCTGT GGGCAGTGTG 
TCGAAACTGC AAATCCTTAA TGCTTCCAGT GATGTTCTAC GGGTCACTTG GGTAGGAGTC 
CCTGGGGCCA CATCCTATAA ACTGGCGTGG GGACGTAGTG AAGGTGGCCC TATGAAACAC 
CGGATACTCC CAGGAAACAA AGAGTCTGCA GAGATACGGG ATCTCGAAGG AGGAGTCAGC 
TACTCCGTGA GAGTGACGGC GCTTGTTGGA GACCGAGAGG GTGCACCGGT GTCCATTGTC 
ATCACCACAC CGCCTGCGAC CCCAGCACTT CTGGAGACTC TTCAAGTTGT GCAGAGCGGG 
GAGCACTCCT TGAGGCTGCG ATGGGAACCA GTACCCGGAG CACCAGGCTT CCGCCTGCAC 
TGGCAACCTG AGGGTGGCCA GGAACAGTCC CTGACCTTGG GACCTGAGTC TAACAGCTAC 
AACCTGGTTG GTCTGGAGCC AGCAACAAAG TACCAAGTAT GGCTGACTGT CCTGGGCCAG 
ACTGGAGAAG GTCCCCCTAG GAAGGTGACT GCATACACTG AGCCCTCTCA CATCCCGAGC 
ACTGAACTAC GCGTGGTGGA TACCTCTATT GACTCAGTGA CTTTGACCTG GACCCCAGTG 
TCTGGGGCAT CCAGTTATAT CTTGTCCTGG CGGCCGCTCA GAGGAACTGG CCAGGAAGTG 
CCGAGGGCCC CACAGACACT GCCAGGGACC TCAAGCTCCC ACAGAGTGAC AGGGCTGGAA 
CCTGGTATTT CCTATGTTTT CTCTCTGACA CCTATCCAGA GCGGTGTACG AGGTTCTGAG 
ATCTCTGTCA CACAAACACC AGCGTGTTCC CATGGCCCAG TGGACGTGGT GTTCCTCTTG 
CATGCCACTC GAGACAATGC TCACAACGCA GAGGCTGTGA GGAGGGTCCT GGAAAGGCTG 
GTGTCTGCAC TTGGGCCTCT TGGTCCACAG GCTGCTCAGG TCGGCCTGCT GACTTACAGC 
CACCGGCCCT CCCCACTGTT CCCACTGAAT AGTTCCCATG ATCTTGGCAT CATCTTGCGA 
AAGATCCGTG ACATCCCCTA CGTGGACCCA AGTGGTAACA ACCTCGGCAC AGCCGTGACC 
ACAGCTCACA GATACCTCTT AGCATCCAAT GCTCCTGGTC GCCGTCAGCA AGTGCCAGGC 
GTGATGGTCC TGTTAGTGGA TGAGCCTCTG AGAGGGGACA TACTCAGCCC CATTCGTGAG 
GCCCAGACTT CTGGGCTTAA GGTGATGGCA CTGAGTTTGG TGGGAGCTGA CCCAGAGCAG 
CTGCGTCGCT TGGCACCAGG CACGGACCCC ATCCAGAACT TCTTTGCTGT GGATAATGGC 
CCAGGCTTGG ACAGAGCAGT CAGTGATCTG GCTGTAGCCC TGTGTCAGGC AGCTGTGACC 
ATAGAGCCGC AAACAGGGCC TTGTGCTGTG CACTGTCCAA AGGGCCAGAA GGGGGAGCCT 
GGAGTAACGG GACTGCAAGG ACAGGCTGGA CCTCCTGGCC CCCCTGGTCT CCCGGGCAGG 
ACCGGTGCTC CAGGCCCGCA AGGTCCTCCT GGAAGTACCC AGGCAAAAGG CGAGAGGGGC 
TTTCCTGGCC CAGAAGGGCC TCCAGGCAGT CCTGGCCTCC CTGGAGTTCC TGGGTCTCCT 
GGCATTAAGG GCTCCACGGG GCGGCCTGGC CCTCGTGGGG AACAGGGAGA ACGAGGGCCT 
CAAGGTCCAA AGGGGGAGCC GGGTGAGCCT GGGCAAATCA CTGGCGGTGG AGGGCCAGGG 
TTTCCTGGAA AGAAAGGGGA CCCTGGACCT TCGGGGCCCC CTGGATCTCG TGGCCCTGTG 
GGGGACCCAG GACCCCGTGG CCCCCCAGGG CTTCCTGGAA TATCAGTGAA GGGTGACAAA 
GGTGATCGTG GGGAACGGGG TCCCCCGGGA CCAGGCATTG GTGCCAGTGA ACAGGGTGAC 
CCTGGACTTC CGGGGCTTCC TGGAAGCCCT GGACCCCAAG GCCCAGCTGG CCGACCTGGA 
GAGAAGGGAG AAAAGGGTGA CTGTGAGGAT GGAGGTCCAG GCCTCCCAGG ACAGCCTGGG 
CCCCCAGGGG AGCCGGGCCT GCGGGGAGCT CCTGGAATGA CTGGGCCCAA AGGTGATCGG 
GGACTGACGG GGACCCCGGG CGAGCCTGGA GTAAAGGGTG AACGGGGACA TCCTGGTCCC 
GTGGGACCCC AGGGGCTCCC CGGGGCTGCT GGACACCCTG GAGTGGAGGG TCCTGAGGGG 
CCACCAGGAC CCACTGGCCG CCGAGGAGAA AAGGGAGAAC CTGGTCGCCC TGGGGATCCT 
GCAGTGGGTC CTGGTGGTGC TGGAGCCAAA GGAGAAAAGG GAGAGGCTGG GCTCCCTGGG 
CCCAGAGGAG CTTCTGGAAG CAAAGGAGAG CAGGGCGCAC CTGGATTGGC TCTTCCTGGA 
GATCCTGGCC CCAAAGGAGA CCCTGGAGAC AGGGGTCCCA TTGGCCTCAC TGGAAGGGCA 
GGACCCACAG GTGACTCAGG GCCTCCTGGA GAGAAAGGAG AGCCTGGTCG ACCTGGTTCC 
CCAGGACCTG TTGGCCCCCG AGGAAGAGAT GGTGAAGCTG GAGAGAAAGG TGACGAGGGG 
ATCCCGGGTG AGCCAGGTTT GCCTGGAAAA GCAGGTGAAC GTGGCCTCCG GGGGGCACCT 
GGGCCTCGGG GGCCTGTGGG TGAGAAGGGG GACCAGGGAG ATCCTGGAGA AGACGGACGG 
AATGGCAGCC CTGGATCATC TGGACCCAAG GGTGACCGTG GGGAGCCTGG TCCCCCAGGT 
CCCCCTGGAC GGCTGGTGGA TGCAGGCATT GAATCCAGAG ACAAGGGAGA GCCTGGACAA 
GAAGGTCCCC GAGGACCCAA GGGTGACCCT GGGCCCCCTG GAGTCTCTGG AGAAAGGGGC 
ATTGATGGGC TTCGGGGACC CCCAGGCCCA CAGGGAGACC CCGGTGTTCG AGGCCCAGCA 
GGAGACAAGG GTGATCGGGG ACCCCCAGGG CTGGATGGCC GGAGTGGGCT GGATGGGAAA 
CCCGGAGCCC CTGGCCCCCC AGGGCTACAT GGTGCTTCAG GCAAAGCTGG GGACCCGGGG 
AGAGATGGAC TTCCAGGCCT TCGAGGAGAA CATGGCCCCC CTGGTCCCCC TGGCCCTCCT 
GGAGTTCCGG GAAAGGCAGG CGATGATGGC AAACCAGGCC TGAATGGGAA AAACGGAGAC 
CCTGGAGACC CTGGAGAAGA TGGAAGGAAG GGAGAAAAGG GAGATTCGGG TGCCCCTGGA 
AGAGAAGGCC CTGATGGCCC CAAGGGTGAA CGTGGAGCTC CTGGCAATCC TGGACTCCAG 
GGTCCTCCGG GTCTCCCAGG GCAGGTCGGT CCTCCCGGCC AGGGTTTCCC TGGTGTCCCA 
GGAATTACGG GTCCTAAGGG TGACCGTGGA GAGACTGGAT CCAAAGGGGA ACAGGGCCTT 
CCTGGAGAAC GTGGCCTACG AGGAGAACCT GGGAGCTTGC CAAACGCGGA GCGTTTGCTG 
GAAACTGCTG GCATCAAGGT GTCGGCCCTG CGGGAGATCG TGGACACCTG GGATGAGAGT 
TCTGGCAGCT TCCTACCGGT GCCTGAGCGG AGACCTGGCC CCAAGGGGGA CCCTGGTGAC 
CGAGGCCCTC CAGGCAAGGA GGGCCTCATC GGCTTTCCTG GAGAACGTGG ACTGAAGGGA 
GAGCGTGGAG ACCCTGGCCC TCAGGGGCCT CCTGGCCTGG CCCTGGGTGA GAGGGGCCCA 
CCTGGCCCAC CCGGCCTTGC GGGGGAACCC GGAAAGCCTG GCATTCCTGG ACTCCCAGGC 
CGGGCTGGTG GTTCAGGGGA AGCAGGAAGG CCAGGAGAGA GGGGAGAAAG GGGAGAGAAA 
GGAGAACGCG GGGATCAGGG CAGAGATGGC CTTCCCGGCC TCCCTGGACC ACCTGGCCCT 
CCTGGCCCCA AGGTGGCCAT TGAAGAGCCA GGCCCTGGAC TGGCTAGAGA GCAAGGACCT 
CCTGGACTCA AGGGTGCCAA GGGGGAGCCA GGCAGTGATG GTGACCCTGG ACCTAAAGGA 
GACAGGGGTG TACCAGGCAT CAAAGGCGAT GTGGGAGAGC CTGGAAAGAG GGGTCATGAT 
GGCAACCCAG GTCTCCCGGG AGAGCGTGGT GTGGCTGGGC CTGAAGGGAA GCCGGGTCTT 
CAGGGCCCAA GGGGGACCCC TGGCCCAGTG GGTAGCCATG GAGACCCTGG ACCACCTGGT 
GCCCCGGGTC TTGCTGGCCC TGCGGGACCC CAGGGGCCTT CTGGCCTGAA GGGGGAGCCT 
GGAGAGACAG GACCTCCAGG ACGGGGTCTG CCTGGACCTG TTGGAGCTGT GGGACTCCCT 
GGCCCTCCTG GCCCTTCCGG CCTCGTGGGT CCACAAGGCT CTCCAGGTTT GCCCGGACAA 
GTGGGGGAGA CAGGGAAGCC AGGACCTCCA GGCCGTGACG GTAGCAGTGG AAAGGACGGA 
GATCGGGGAA GTCCTGGTGT GCCGGGGTCA CCAGGTCTGC CCGGCCCTGT TGGACCTAAA 
GGAGAACCTG GGCCTGTGGG GGCTCCTGGA CAGGTGGTGG TCGGGCCCCC TGGAGCAAAA 
GGCGAGAAGG GAGCCCCGGG AGACCTTGCT GGAGCCCTGC TGGGTGAGCC GGGAGCCAAA 
GGTGACCGAG GACTGCCTGG ACCACGGGGT GAAAAGGGTG AAGCTGGCCG TGCAGGAGGA 
CCTGGGGACC CTGGGGAAGA TGGTCAAAAA GGGGCTCCAG GACTCAAAGG TCTTAAGGGT 
GAGCCAGGCA TTGGGGTTCA GGGCCCCCCT GGCCCAAGTG GTCCTCCAGG TATGAAGGGA 
GACTTGGGCC CCCCTGGTGC CCCCGGGGCT CCTGGTGTTG TTGGGTTCCC TGGTCAGACA 
GGACCTCGAG GAGAGACGGG CCAGCCAGGG CCTGTTGGAG AGAGGGGTCT GGCAGGCCCT 
CCAGGGAGAG AAGGTGCCCC AGGTCCCTTG GGACCACCTG GACCACCAGG GTCAGCGGGA 
GCACCTGGCG CCTCTGGACT CAAAGGAGAC AAGGGAGACC CGGGAGCAGG ACTGCCGGGG 
CCCCGAGGTG AGCGTGGTGA GCCAGGTGTC CGGGGTGAAG ATGGCCACCC TGGCCAGGAA 
GGACCTCGTG GACTTGTGGG GCCCCCTGGC AGCCGGGGAG AGCAAGGAGA GAAGGGCGCT 
GCTGGCGCTG CAGGGCTCAA GGGTGACAAG GGTGACTCAG CTGTGATTGA GGGACCTCCC 
GGGCCACGAG GTGCCAAAGG GGATATGGGT GAACGAGGGC CTCGGGGCAT AGATGGTGAC 
AAAGGACCTC GGGGAGAAAG TGGGAATCCT GGTGACAAGG GTTCCAAAGG AGAGCCAGGT 
GACAAAGGCT CAGCTGGATC GATTGGAGTT CGAGGACTCA CAGGACCCAA GGGAGAGCCA 
GGTGCTGCAG GGATCCCCGG TGAGCCGGGA GCCCCAGGAA AGGATGGAAT CCCTGGTTTC 
CGAGGAGACA AAGGAGATAT TGGCTTCATG GGTCCCAGGG GCCTCAAGGG TGAAAAAGGA 
ATAAAGGGAA CCTGTGGCCG TGACGGAGAG AGAGGAGATA AGGGAGAAGC TGGTTTTCCT 
GGTCGCCCTG GGTTGGCAGG AAAGAAAGGA GACATGGGGG AGCCTGGTCT GCCGGGTCAG 
TCTGGGGCTC CTGGGAAAGA AGGCCTGATC GGTCCCAAGG GTGACAGAGG CTTTGATGGG 
CAGTCAGGAC CCAAGGGTGA CCAAGGCGAG AAAGGGGAGA GGGGGCCTCC AGGAGTTGGG 
GGCTTCCCAG GTCCCAGAGG AAACGATGGC TCTAGTGGTC CCCCAGGGCC ACCTGGTGGT 
GTTGGTCCCA AGGGACCCGA AGGGCTTCAG GGTCAGAAGG GTGAGCGAGG TCCCCCTGGA 
GAGAGCGTGG TGGGGGCTCC TGGTGCTCCT GGCACCCCTG GAGAGAGAGG AGAGCAGGGA 
CGACCAGGAC CTGCTGGCCC CCGTGGGGAG AAGGGAGAAG CTGCGCTGAC TGAGGATGAC 
ATTCGGGACT TTGTGCGTCA GGAGATGAGT CAGCACTGTG CCTGCCAGGG CCAGTTTATT 
GCGTCTGGAT CACGACCCCT CCCCGGTTAT GCTGCGGATA CAGCTGGTTC CCAGCTTCAC 
CATGTTCCTG TGCTCCGGGT CTCTCATGTT GAGGAGGAAG GCCAGGTGCC CCCTGAAGAT 
GACGATGACT TCTCTGAGTA CTCTGTGTAT TCTGTGGAGG ACTACCAGGA GCCTGAAGTT 
CCATGGGATG GTGAAGCCGA GATTAAGGGC TGGGACCAGA GAGGTTCAGA TCTCTGCTCC 
CTACCCTTGG ATGAGGGCTC CTGTACTGCC TATACCCTGC GCTGGTACCA TCGAGCTGTG 
CCTGGTGGCA CAGCCTGTCA TCCCTTTGTC TATGGTGGCT GCGGAGGGAA TGCCAATCGT 
TTTGGAACCC GTGAGGCGTG TGAGCGCCGC TGCCCACCCC AAGGGGTCCA CAGCCAGAAA 
ACAGGTGCTG CCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Col7a1 ( XM_006511937.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006512000.1 Mus musculus collagen, type VII, alpha 1 (Col7a1), transcript variant X3, mRNA.

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CloneID	OMu43293
Clone ID Related Accession (Same CDS sequence)	XM_006511937.2
Accession Version	XM_006511937.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7392bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	collagen alpha-1(VII) chain isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000075.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Feb 9, 2015 this sequence version replaced XM_006511937.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGCTGC GACTCCTGGT CGCTGCGCTC TGCGCTGCAG AGATCCTGAT GGGAGCGCCG 
GAAGTGTGGG CCCAGCCCAG AGATAGAGTG ACCTGCACAC GCCTTTATGC TGCTGACATC 
GTGTTCTTAC TCGATGGCTC GTCGTCCATT GGCCGCAGCA ACTTTCGAGA AGTGCGAGGA 
TTCCTGGAGG GGCTGGTGCT GCCTTTCTCA GGAGCAGCCA GTGCACAGGG CGTCCGGTTT 
GCCACAGTGC AGTACAGTGA TGATCCACAG ACAGAATTTG GTCTGGATAC ACTTGGCTCT 
GGGAGTGACA CCATCCGAGC CATCCGTGAG CTCAGCTATA AAGGTGGTAA CACTCGCACG 
GGGGCCGCTC TTCACCACGT TTCTGACCGT GTCTTCCTGC CTCGTCTAAC ACGTCCTGGT 
GTCCCCAAGG TCTGCATCCT GATCACAGAT GGGAAATCCC AAGATTTAGT GGACACAGCT 
GCCCAAAAGC TGAAGGGGCA GGGAGTCAAG CTGTTTGCTG TGGGAATCAA GAATGCCGAC 
CCTGAGGAAC TGAAGCGTGT GGCCTCACAG CCAACCAGCG ATTTCTTCTT CTTCGTCAAT 
GACTTCAGCA TCTTGAGGAC CCTGTTGCCT CTCATTTCCA GGAGGGTGTG CACAACTGCT 
GGTGGTGTGC CTGTGACCCT GCCTTCTGAT GACACTCCGT CTGGGCCCCG GGACCTGGTG 
CTCTCTGAGC CAAGTAGCCA ATCCTTGCGA GTACAATGGA CAGCAGCCAG TGGCCCTGTG 
ACTGGCTACA AGGTTCAGTA CACACCTCTG ACAGGGCTAG GGCAGCCACT GCCAAGTGAG 
CGACAGGAGG TGAACATCCC GGCTGGTGAG ACCAGCACGC GTCTCCAGGG TCTCCGGCCA 
CTAACGGACT ACCAAGTGAC TGTGGTTGCC CTCTATGCCA ACAGCATCGG GGAGGCCGTG 
AGTGGGACAG CTCGGACCAC TGCCAAGGAA GGGCTAGAAT TGAGCCTCCA GAACATCACA 
TCGCACAGCC TCCTGGTGGC CTGGAGGAGG GTACCAGGTG CTAATGGCTA CCGTGTAACA 
TGGCGGGACC TCAGTGGTGG GCCAACCCAG CAGCAGGACC TGAGCCCTGG ACAGGGGTCA 
GTGTTTCTAG ATCACTTGGA GCCTGGCACG GACTATGAGG TGACCGTGAG CGCCCTATTT 
GGCCACAGCG TGGGGCCTGC CGCTTCTCTG ACTGCCCGCA CCGCATCTTC TGTTGAGCAA 
ACCCTGCATC CCATCATCTT GAGCCCGACA TCCATCCTCC TTTCCTGGAA CTTGGTGCCT 
GAGGCCCGCG GCTACCGCTT GGAGTGGCGT CGTGAGAGTG GCTTGGAAAC ACCACAGAAA 
GTGGAACTGC CCCCTGATGT GACCCGCCAT CAGCTGGATG GTTTGCAGCC AGGCACTGAG 
TACCGTCTCA CGCTGTATAC TCTGCTGGAG GGCCGTGAGG TGGCCACTCC TGCAACCGTG 
GTTCCTACAG GGCTAGAGCA GCTGGTGAGC CCGGTGATGA ATTTACAAGC CATAGAACTA 
CCTGGGCAGC GGGTGCGAGT GTCTTGGAAC CCAGTCCCTG GGGCCACTGA GTACCGTTTC 
ACTGTACGCA CCACCCAGGG GGTCGAGCGG ACTCTATTGC TTCCTGGGAG CCAGACAACC 
TTTGACTTAG ATGATGTTCG AGCTGGGCTT AGCTACACGG TGCGGGTGTC TGCTCGAGTG 
GGTGCTCAGG AGGGTGATGC CAGCATCCTA ACGATCCACA GAGATCCAGA AGCCCCACTT 
GTTGTCCCAG GACTGCGGGT TGTCGCATCA GATGCAACAA GAATTAGGGT GGCCTGGGGA 
CTTGTCCCTG GAGCCAGTGG ATTTCGGATC AGCTGGAGGA CAGGCAGTGG TCCAGAGTCC 
AGCCGGACGC TGACCCCAGA CTCCACTGTC ACGGACATCC TGGGGTTGCA GCCTAGCACC 
TCTTATCAAG TCGCTGTCTC AGCACTTAGA GGGAGAGAAG AGGGGCCCCC TGTGGTCATC 
GTGGCTAGAA CTGATCCACT GGGCCCAGTG AGGAGAGTCC ACTTGACTCA GGCTGGCAGC 
TCATCTGTCA GCATCACCTG GACTGGGGTT CCCGGTGCCA CAGGATACAG GGTTTCCTGG 
CACTCAGGCC ATGGTCCAGA GAAATCTCTG TTGGTTTCTG GAGATGCCAC GGTGGCTGAA 
ATAGATGGGC TGGAGCCGGA CACAGAGTAT ATAGTGCGTG TGAGAACCCA CGTAGCTGGT 
GTGGATGGAG CCCCCGCCTC TGTGGTTGTG AGGACTGCTC CTGAGCCTGT GGGCAGTGTG 
TCGAAACTGC AAATCCTTAA TGCTTCCAGT GATGTTCTAC GGGTCACTTG GGTAGGAGTC 
CCTGGGGCCA CATCCTATAA ACTGGCGTGG GGACGTAGTG AAGGTGGCCC TATGAAACAC 
CGGATACTCC CAGGAAACAA AGAGTCTGCA GAGATACGGG ATCTCGAAGG AGGAGTCAGC 
TACTCCGTGA GAGTGACGGC GCTTGTTGGA GACCGAGAGG GTGCACCGGT GTCCATTGTC 
ATCACCACAC CGCCTGCGAC CCCAGCACTT CTGGAGACTC TTCAAGTTGT GCAGAGCGGG 
GAGCACTCCT TGAGGCTGCG ATGGGAACCA GTACCCGGAG CACCAGGCTT CCGCCTGCAC 
TGGCAACCTG AGGGTGGCCA GGAACAGTCC CTGACCTTGG GACCTGAGTC TAACAGCTAC 
AACCTGGTTG GTCTGGAGCC AGCAACAAAG TACCAAGTAT GGCTGACTGT CCTGGGCCAG 
ACTGGAGAAG GTCCCCCTAG GAAGGTGACT GCATACACTG AGCCCTCTCA CATCCCGAGC 
ACTGAACTAC GCGTGGTGGA TACCTCTATT GACTCAGTGA CTTTGACCTG GACCCCAGTG 
TCTGGGGCAT CCAGTTATAT CTTGTCCTGG CGGCCGCTCA GAGGAACTGG CCAGGAAGTG 
CCGAGGGCCC CACAGACACT GCCAGGGACC TCAAGCTCCC ACAGAGTGAC AGGGCTGGAA 
CCTGGTATTT CCTATGTTTT CTCTCTGACA CCTATCCAGA GCGGTGTACG AGGTTCTGAG 
ATCTCTGTCA CACAAACACC AGCGTGTTCC CATGGCCCAG TGGACGTGGT GTTCCTCTTG 
CATGCCACTC GAGACAATGC TCACAACGCA GAGGCTGTGA GGAGGGTCCT GGAAAGGCTG 
GTGTCTGCAC TTGGGCCTCT TGGTCCACAG GCTGCTCAGG TCGGCCTGCT GACTTACAGC 
CACCGGCCCT CCCCACTGTT CCCACTGAAT AGTTCCCATG ATCTTGGCAT CATCTTGCGA 
AAGATCCGTG ACATCCCCTA CGTGGACCCA AGTGGTAACA ACCTCGGCAC AGCCGTGACC 
ACAGCTCACA GATACCTCTT AGCATCCAAT GCTCCTGGTC GCCGTCAGCA AGTGCCAGGC 
GTGATGGTCC TGTTAGTGGA TGAGCCTCTG AGAGGGGACA TACTCAGCCC CATTCGTGAG 
GCCCAGACTT CTGGGCTTAA GGTGATGGCA CTGAGTTTGG TGGGAGCTGA CCCAGAGCAG 
CTGCGTCGCT TGGCACCAGG CACGGACCCC ATCCAGAACT TCTTTGCTGT GGATAATGGC 
CCAGGCTTGG ACAGAGCAGT CAGTGATCTG GCTGTAGCCC TGTGTCAGGC AGCTGTGACC 
ATAGAGCCGC AAACAGGGCC TTGTGCTGTG CACTGTCCAA AGGGCCAGAA GGGGGAGCCT 
GGAGTAACGG GACTGCAAGG ACAGGCTGGA CCTCCTGGCC CCCCTGGTCT CCCGGGCAGG 
ACCGGTGCTC CAGGCCCGCA AGGTCCTCCT GGAAGTACCC AGGCAAAAGG CGAGAGGGGC 
TTTCCTGGCC CAGAAGGGCC TCCAGGCAGT CCTGGCCTCC CTGGAGTTCC TGGGTCTCCT 
GGCATTAAGG GCTCCACGGG GCGGCCTGGC CCTCGTGGGG AACAGGGAGA ACGAGGGCCT 
CAAGGTCCAA AGGGGGAGCC GGGTGAGCCT GGGCAAATCA CTGGCGGTGG AGGGCCAGGG 
TTTCCTGGAA AGAAAGGGGA CCCTGGACCT TCGGGGCCCC CTGGATCTCG TGGCCCTGTG 
GGGGACCCAG GACCCCGTGG CCCCCCAGGG CTTCCTGGAA TATCAGTGAA GGGTGACAAA 
GGTGATCGTG GGGAACGGGG TCCCCCGGGA CCAGGCATTG GTGCCAGTGA ACAGGGTGAC 
CCTGGACTTC CGGGGCTTCC TGGAAGCCCT GGACCCCAAG GCCCAGCTGG CCGACCTGGA 
GAGAAGGGAG AAAAGGGTGA CTGTGAGGAT GGAGGTCCAG GCCTCCCAGG ACAGCCTGGG 
CCCCCAGGGG AGCCGGGCCT GCGGGGAGCT CCTGGAATGA CTGGGCCCAA AGGTGATCGG 
GGACTGACGG GGACCCCGGG CGAGCCTGGA GTAAAGGGTG AACGGGGACA TCCTGGTCCC 
GTGGGACCCC AGGGGCTCCC CGGGGCTGCT GGACACCCTG GAGTGGAGGG TCCTGAGGGG 
CCACCAGGAC CCACTGGCCG CCGAGGAGAA AAGGGAGAAC CTGGTCGCCC TGGGGATCCT 
GCAGTGGGTC CTGGTGGTGC TGGAGCCAAA GGAGAAAAGG GAGAGGCTGG GCTCCCTGGG 
CCCAGAGGAG CTTCTGGAAG CAAAGGAGAG CAGGGCGCAC CTGGATTGGC TCTTCCTGGA 
GATCCTGGCC CCAAAGGAGA CCCTGGAGAC AGGGGTCCCA TTGGCCTCAC TGGAAGGGCA 
GGACCCACAG GTGACTCAGG GCCTCCTGGA GAGAAAGGAG AGCCTGGTCG ACCTGGTTCC 
CCAGGACCTG TTGGCCCCCG AGGAAGAGAT GGTGAAGCTG GAGAGAAAGG TGACGAGGGG 
ATCCCGGGTG AGCCAGGTTT GCCTGGAAAA GCAGGTGAAC GTGGCCTCCG GGGGGCACCT 
GGGCCTCGGG GGCCTGTGGG TGAGAAGGGG GACCAGGGAG ATCCTGGAGA AGACGGACGG 
AATGGCAGCC CTGGATCATC TGGACCCAAG GGTGACCGTG GGGAGCCTGG TCCCCCAGGT 
CCCCCTGGAC GGCTGGTGGA TGCAGGCATT GAATCCAGAG ACAAGGGAGA GCCTGGACAA 
GAAGGTCCCC GAGGACCCAA GGGTGACCCT GGGCCCCCTG GAGTCTCTGG AGAAAGGGGC 
ATTGATGGGC TTCGGGGACC CCCAGGCCCA CAGGGAGACC CCGGTGTTCG AGGCCCAGCA 
GGAGACAAGG GTGATCGGGG ACCCCCAGGG CTGGATGGCC GGAGTGGGCT GGATGGGAAA 
CCCGGAGCCC CTGGCCCCCC AGGGCTACAT GGTGCTTCAG GCAAAGCTGG GGACCCGGGG 
AGAGATGGAC TTCCAGGCCT TCGAGGAGAA CATGGCCCCC CTGGTCCCCC TGGCCCTCCT 
GGAGTTCCGG GAAAGGCAGG CGATGATGGC AAACCAGGCC TGAATGGGAA AAACGGAGAC 
CCTGGAGACC CTGGAGAAGA TGGAAGGAAG GGAGAAAAGG GAGATTCGGG TGCCCCTGGA 
AGAGAAGGCC CTGATGGCCC CAAGGGTGAA CGTGGAGCTC CTGGCAATCC TGGACTCCAG 
GGTCCTCCGG GTCTCCCAGG GCAGGTCGGT CCTCCCGGCC AGGGTTTCCC TGGTGTCCCA 
GGAATTACGG GTCCTAAGGG TGACCGTGGA GAGACTGGAT CCAAAGGGGA ACAGGGCCTT 
CCTGGAGAAC GTGGCCTACG AGGAGAACCT GGGAGCTTGC CAAACGCGGA GCGTTTGCTG 
GAAACTGCTG GCATCAAGGT GTCGGCCCTG CGGGAGATCG TGGACACCTG GGATGAGAGT 
TCTGGCAGCT TCCTACCGGT GCCTGAGCGG AGACCTGGCC CCAAGGGGGA CCCTGGTGAC 
CGAGGCCCTC CAGGCAAGGA GGGCCTCATC GGCTTTCCTG GAGAACGTGG ACTGAAGGGA 
GAGCGTGGAG ACCCTGGCCC TCAGGGGCCT CCTGGCCTGG CCCTGGGTGA GAGGGGCCCA 
CCTGGCCCAC CCGGCCTTGC GGGGGAACCC GGAAAGCCTG GCATTCCTGG ACTCCCAGGC 
CGGGCTGGTG GTTCAGGGGA AGCAGGAAGG CCAGGAGAGA GGGGAGAAAG GGGAGAGAAA 
GGAGAACGCG GGGATCAGGG CAGAGATGGC CTTCCCGGCC TCCCTGGACC ACCTGGCCCT 
CCTGGCCCCA AGGTGGCCAT TGAAGAGCCA GGCCCTGGAC TGGCTAGAGA GCAAGGACCT 
CCTGGACTCA AGGGTGCCAA GGGGGAGCCA GGCAGTGATG GTGACCCTGG ACCTAAAGGA 
GACAGGGGTG TACCAGGCAT CAAAGGCGAT GTGGGAGAGC CTGGAAAGAG GGGTCATGAT 
GGCAACCCAG GTCTCCCGGG AGAGCGTGGT GTGGCTGGGC CTGAAGGGAA GCCGGGTCTT 
CAGGGCCCAA GGGGGACCCC TGGCCCAGTG GGTAGCCATG GAGACCCTGG ACCACCTGGT 
GCCCCGGGTC TTGCTGGCCC TGCGGGACCC CAGGGGCCTT CTGGCCTGAA GGGGGAGCCT 
GGAGAGACAG GACCTCCAGG ACGGGGTCTG CCTGGACCTG TTGGAGCTGT GGGACTCCCT 
GGCCCTCCTG GCCCTTCCGG CCTCGTGGGT CCACAAGGCT CTCCAGGTTT GCCCGGACAA 
GTGGGGGAGA CAGGGAAGCC AGGACCTCCA GGCCGTGACG GTAGCAGTGG AAAGGACGGA 
GATCGGGGAA GTCCTGGTGT GCCGGGGTCA CCAGGTCTGC CCGGCCCTGT TGGACCTAAA 
GGAGAACCTG GGCCTGTGGG GGCTCCTGGA CAGGTGGTGG TCGGGCCCCC TGGAGCAAAA 
GGCGAGAAGG GAGCCCCGGG AGACCTTGCT GGAGCCCTGC TGGGTGAGCC GGGAGCCAAA 
GGTGACCGAG GACTGCCTGG ACCACGGGGT GAAAAGGGTG AAGCTGGCCG TGCAGGAGGA 
CCTGGGGACC CTGGGGAAGA TGGTCAAAAA GGGGCTCCAG GACTCAAAGG TCTTAAGGGT 
GAGCCAGGCA TTGGGGTTCA GGGCCCCCCT GGCCCAAGTG GTCCTCCAGG TATGAAGGGA 
GACTTGGGCC CCCCTGGTGC CCCCGGGGCT CCTGGTGTTG TTGGGTTCCC TGGTCAGACA 
GGACCTCGAG GAGAGACGGG CCAGCCAGGG CCTGTTGGAG AGAGGGGTCT GGCAGGCCCT 
CCAGGGAGAG AAGGTGCCCC AGGTCCCTTG GGACCACCTG GACCACCAGG GTCAGCGGGA 
GCACCTGGCG CCTCTGGACT CAAAGGAGAC AAGGGAGACC CGGGAGCAGG ACTGCCGGGG 
CCCCGAGGGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006512000.1
CDS	64..7455
Translation	MRLRLLVAALCAAEILMGAPEVWAQPRDRVTCTRLYAADIVFLLDGSSSIGRSNFREVRGFLEGLVLPFSGAASAQGVRFATVQYSDDPQTEFGLDTLGSGSDTIRAIRELSYKGGNTRTGAALHHVSDRVFLPRLTRPGVPKVCILITDGKSQDLVDTAAQKLKGQGVKLFAVGIKNADPEELKRVASQPTSDFFFFVNDFSILRTLLPLISRRVCTTAGGVPVTLPSDDTPSGPRDLVLSEPSSQSLRVQWTAASGPVTGYKVQYTPLTGLGQPLPSERQEVNIPAGETSTRLQGLRPLTDYQVTVVALYANSIGEAVSGTARTTAKEGLELSLQNITSHSLLVAWRRVPGANGYRVTWRDLSGGPTQQQDLSPGQGSVFLDHLEPGTDYEVTVSALFGHSVGPAASLTARTASSVEQTLHPIILSPTSILLSWNLVPEARGYRLEWRRESGLETPQKVELPPDVTRHQLDGLQPGTEYRLTLYTLLEGREVATPATVVPTGLEQLVSPVMNLQAIELPGQRVRVSWNPVPGATEYRFTVRTTQGVERTLLLPGSQTTFDLDDVRAGLSYTVRVSARVGAQEGDASILTIHRDPEAPLVVPGLRVVASDATRIRVAWGLVPGASGFRISWRTGSGPESSRTLTPDSTVTDILGLQPSTSYQVAVSALRGREEGPPVVIVARTDPLGPVRRVHLTQAGSSSVSITWTGVPGATGYRVSWHSGHGPEKSLLVSGDATVAEIDGLEPDTEYIVRVRTHVAGVDGAPASVVVRTAPEPVGSVSKLQILNASSDVLRVTWVGVPGATSYKLAWGRSEGGPMKHRILPGNKESAEIRDLEGGVSYSVRVTALVGDREGAPVSIVITTPPATPALLETLQVVQSGEHSLRLRWEPVPGAPGFRLHWQPEGGQEQSLTLGPESNSYNLVGLEPATKYQVWLTVLGQTGEGPPRKVTAYTEPSHIPSTELRVVDTSIDSVTLTWTPVSGASSYILSWRPLRGTGQEVPRAPQTLPGTSSSHRVTGLEPGISYVFSLTPIQSGVRGSEISVTQTPACSHGPVDVVFLLHATRDNAHNAEAVRRVLERLVSALGPLGPQAAQVGLLTYSHRPSPLFPLNSSHDLGIILRKIRDIPYVDPSGNNLGTAVTTAHRYLLASNAPGRRQQVPGVMVLLVDEPLRGDILSPIREAQTSGLKVMALSLVGADPEQLRRLAPGTDPIQNFFAVDNGPGLDRAVSDLAVALCQAAVTIEPQTGPCAVHCPKGQKGEPGVTGLQGQAGPPGPPGLPGRTGAPGPQGPPGSTQAKGERGFPGPEGPPGSPGLPGVPGSPGIKGSTGRPGPRGEQGERGPQGPKGEPGEPGQITGGGGPGFPGKKGDPGPSGPPGSRGPVGDPGPRGPPGLPGISVKGDKGDRGERGPPGPGIGASEQGDPGLPGLPGSPGPQGPAGRPGEKGEKGDCEDGGPGLPGQPGPPGEPGLRGAPGMTGPKGDRGLTGTPGEPGVKGERGHPGPVGPQGLPGAAGHPGVEGPEGPPGPTGRRGEKGEPGRPGDPAVGPGGAGAKGEKGEAGLPGPRGASGSKGEQGAPGLALPGDPGPKGDPGDRGPIGLTGRAGPTGDSGPPGEKGEPGRPGSPGPVGPRGRDGEAGEKGDEGIPGEPGLPGKAGERGLRGAPGPRGPVGEKGDQGDPGEDGRNGSPGSSGPKGDRGEPGPPGPPGRLVDAGIESRDKGEPGQEGPRGPKGDPGPPGVSGERGIDGLRGPPGPQGDPGVRGPAGDKGDRGPPGLDGRSGLDGKPGAPGPPGLHGASGKAGDPGRDGLPGLRGEHGPPGPPGPPGVPGKAGDDGKPGLNGKNGDPGDPGEDGRKGEKGDSGAPGREGPDGPKGERGAPGNPGLQGPPGLPGQVGPPGQGFPGVPGITGPKGDRGETGSKGEQGLPGERGLRGEPGSLPNAERLLETAGIKVSALREIVDTWDESSGSFLPVPERRPGPKGDPGDRGPPGKEGLIGFPGERGLKGERGDPGPQGPPGLALGERGPPGPPGLAGEPGKPGIPGLPGRAGGSGEAGRPGERGERGEKGERGDQGRDGLPGLPGPPGPPGPKVAIEEPGPGLAREQGPPGLKGAKGEPGSDGDPGPKGDRGVPGIKGDVGEPGKRGHDGNPGLPGERGVAGPEGKPGLQGPRGTPGPVGSHGDPGPPGAPGLAGPAGPQGPSGLKGEPGETGPPGRGLPGPVGAVGLPGPPGPSGLVGPQGSPGLPGQVGETGKPGPPGRDGSSGKDGDRGSPGVPGSPGLPGPVGPKGEPGPVGAPGQVVVGPPGAKGEKGAPGDLAGALLGEPGAKGDRGLPGPRGEKGEAGRAGGPGDPGEDGQKGAPGLKGLKGEPGIGVQGPPGPSGPPGMKGDLGPPGAPGAPGVVGFPGQTGPRGETGQPGPVGERGLAGPPGREGAPGPLGPPGPPGSAGAPGASGLKGDKGDPGAGLPGPRG

Target ORF information:

RefSeq Version	XM_006511937.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus collagen, type VII, alpha 1 (Col7a1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006511937.2

ORF Insert Sequence:

ATGAGGCTGC GACTCCTGGT CGCTGCGCTC TGCGCTGCAG AGATCCTGAT GGGAGCGCCG 
GAAGTGTGGG CCCAGCCCAG AGATAGAGTG ACCTGCACAC GCCTTTATGC TGCTGACATC 
GTGTTCTTAC TCGATGGCTC GTCGTCCATT GGCCGCAGCA ACTTTCGAGA AGTGCGAGGA 
TTCCTGGAGG GGCTGGTGCT GCCTTTCTCA GGAGCAGCCA GTGCACAGGG CGTCCGGTTT 
GCCACAGTGC AGTACAGTGA TGATCCACAG ACAGAATTTG GTCTGGATAC ACTTGGCTCT 
GGGAGTGACA CCATCCGAGC CATCCGTGAG CTCAGCTATA AAGGTGGTAA CACTCGCACG 
GGGGCCGCTC TTCACCACGT TTCTGACCGT GTCTTCCTGC CTCGTCTAAC ACGTCCTGGT 
GTCCCCAAGG TCTGCATCCT GATCACAGAT GGGAAATCCC AAGATTTAGT GGACACAGCT 
GCCCAAAAGC TGAAGGGGCA GGGAGTCAAG CTGTTTGCTG TGGGAATCAA GAATGCCGAC 
CCTGAGGAAC TGAAGCGTGT GGCCTCACAG CCAACCAGCG ATTTCTTCTT CTTCGTCAAT 
GACTTCAGCA TCTTGAGGAC CCTGTTGCCT CTCATTTCCA GGAGGGTGTG CACAACTGCT 
GGTGGTGTGC CTGTGACCCT GCCTTCTGAT GACACTCCGT CTGGGCCCCG GGACCTGGTG 
CTCTCTGAGC CAAGTAGCCA ATCCTTGCGA GTACAATGGA CAGCAGCCAG TGGCCCTGTG 
ACTGGCTACA AGGTTCAGTA CACACCTCTG ACAGGGCTAG GGCAGCCACT GCCAAGTGAG 
CGACAGGAGG TGAACATCCC GGCTGGTGAG ACCAGCACGC GTCTCCAGGG TCTCCGGCCA 
CTAACGGACT ACCAAGTGAC TGTGGTTGCC CTCTATGCCA ACAGCATCGG GGAGGCCGTG 
AGTGGGACAG CTCGGACCAC TGCCAAGGAA GGGCTAGAAT TGAGCCTCCA GAACATCACA 
TCGCACAGCC TCCTGGTGGC CTGGAGGAGG GTACCAGGTG CTAATGGCTA CCGTGTAACA 
TGGCGGGACC TCAGTGGTGG GCCAACCCAG CAGCAGGACC TGAGCCCTGG ACAGGGGTCA 
GTGTTTCTAG ATCACTTGGA GCCTGGCACG GACTATGAGG TGACCGTGAG CGCCCTATTT 
GGCCACAGCG TGGGGCCTGC CGCTTCTCTG ACTGCCCGCA CCGCATCTTC TGTTGAGCAA 
ACCCTGCATC CCATCATCTT GAGCCCGACA TCCATCCTCC TTTCCTGGAA CTTGGTGCCT 
GAGGCCCGCG GCTACCGCTT GGAGTGGCGT CGTGAGAGTG GCTTGGAAAC ACCACAGAAA 
GTGGAACTGC CCCCTGATGT GACCCGCCAT CAGCTGGATG GTTTGCAGCC AGGCACTGAG 
TACCGTCTCA CGCTGTATAC TCTGCTGGAG GGCCGTGAGG TGGCCACTCC TGCAACCGTG 
GTTCCTACAG GGCTAGAGCA GCTGGTGAGC CCGGTGATGA ATTTACAAGC CATAGAACTA 
CCTGGGCAGC GGGTGCGAGT GTCTTGGAAC CCAGTCCCTG GGGCCACTGA GTACCGTTTC 
ACTGTACGCA CCACCCAGGG GGTCGAGCGG ACTCTATTGC TTCCTGGGAG CCAGACAACC 
TTTGACTTAG ATGATGTTCG AGCTGGGCTT AGCTACACGG TGCGGGTGTC TGCTCGAGTG 
GGTGCTCAGG AGGGTGATGC CAGCATCCTA ACGATCCACA GAGATCCAGA AGCCCCACTT 
GTTGTCCCAG GACTGCGGGT TGTCGCATCA GATGCAACAA GAATTAGGGT GGCCTGGGGA 
CTTGTCCCTG GAGCCAGTGG ATTTCGGATC AGCTGGAGGA CAGGCAGTGG TCCAGAGTCC 
AGCCGGACGC TGACCCCAGA CTCCACTGTC ACGGACATCC TGGGGTTGCA GCCTAGCACC 
TCTTATCAAG TCGCTGTCTC AGCACTTAGA GGGAGAGAAG AGGGGCCCCC TGTGGTCATC 
GTGGCTAGAA CTGATCCACT GGGCCCAGTG AGGAGAGTCC ACTTGACTCA GGCTGGCAGC 
TCATCTGTCA GCATCACCTG GACTGGGGTT CCCGGTGCCA CAGGATACAG GGTTTCCTGG 
CACTCAGGCC ATGGTCCAGA GAAATCTCTG TTGGTTTCTG GAGATGCCAC GGTGGCTGAA 
ATAGATGGGC TGGAGCCGGA CACAGAGTAT ATAGTGCGTG TGAGAACCCA CGTAGCTGGT 
GTGGATGGAG CCCCCGCCTC TGTGGTTGTG AGGACTGCTC CTGAGCCTGT GGGCAGTGTG 
TCGAAACTGC AAATCCTTAA TGCTTCCAGT GATGTTCTAC GGGTCACTTG GGTAGGAGTC 
CCTGGGGCCA CATCCTATAA ACTGGCGTGG GGACGTAGTG AAGGTGGCCC TATGAAACAC 
CGGATACTCC CAGGAAACAA AGAGTCTGCA GAGATACGGG ATCTCGAAGG AGGAGTCAGC 
TACTCCGTGA GAGTGACGGC GCTTGTTGGA GACCGAGAGG GTGCACCGGT GTCCATTGTC 
ATCACCACAC CGCCTGCGAC CCCAGCACTT CTGGAGACTC TTCAAGTTGT GCAGAGCGGG 
GAGCACTCCT TGAGGCTGCG ATGGGAACCA GTACCCGGAG CACCAGGCTT CCGCCTGCAC 
TGGCAACCTG AGGGTGGCCA GGAACAGTCC CTGACCTTGG GACCTGAGTC TAACAGCTAC 
AACCTGGTTG GTCTGGAGCC AGCAACAAAG TACCAAGTAT GGCTGACTGT CCTGGGCCAG 
ACTGGAGAAG GTCCCCCTAG GAAGGTGACT GCATACACTG AGCCCTCTCA CATCCCGAGC 
ACTGAACTAC GCGTGGTGGA TACCTCTATT GACTCAGTGA CTTTGACCTG GACCCCAGTG 
TCTGGGGCAT CCAGTTATAT CTTGTCCTGG CGGCCGCTCA GAGGAACTGG CCAGGAAGTG 
CCGAGGGCCC CACAGACACT GCCAGGGACC TCAAGCTCCC ACAGAGTGAC AGGGCTGGAA 
CCTGGTATTT CCTATGTTTT CTCTCTGACA CCTATCCAGA GCGGTGTACG AGGTTCTGAG 
ATCTCTGTCA CACAAACACC AGCGTGTTCC CATGGCCCAG TGGACGTGGT GTTCCTCTTG 
CATGCCACTC GAGACAATGC TCACAACGCA GAGGCTGTGA GGAGGGTCCT GGAAAGGCTG 
GTGTCTGCAC TTGGGCCTCT TGGTCCACAG GCTGCTCAGG TCGGCCTGCT GACTTACAGC 
CACCGGCCCT CCCCACTGTT CCCACTGAAT AGTTCCCATG ATCTTGGCAT CATCTTGCGA 
AAGATCCGTG ACATCCCCTA CGTGGACCCA AGTGGTAACA ACCTCGGCAC AGCCGTGACC 
ACAGCTCACA GATACCTCTT AGCATCCAAT GCTCCTGGTC GCCGTCAGCA AGTGCCAGGC 
GTGATGGTCC TGTTAGTGGA TGAGCCTCTG AGAGGGGACA TACTCAGCCC CATTCGTGAG 
GCCCAGACTT CTGGGCTTAA GGTGATGGCA CTGAGTTTGG TGGGAGCTGA CCCAGAGCAG 
CTGCGTCGCT TGGCACCAGG CACGGACCCC ATCCAGAACT TCTTTGCTGT GGATAATGGC 
CCAGGCTTGG ACAGAGCAGT CAGTGATCTG GCTGTAGCCC TGTGTCAGGC AGCTGTGACC 
ATAGAGCCGC AAACAGGGCC TTGTGCTGTG CACTGTCCAA AGGGCCAGAA GGGGGAGCCT 
GGAGTAACGG GACTGCAAGG ACAGGCTGGA CCTCCTGGCC CCCCTGGTCT CCCGGGCAGG 
ACCGGTGCTC CAGGCCCGCA AGGTCCTCCT GGAAGTACCC AGGCAAAAGG CGAGAGGGGC 
TTTCCTGGCC CAGAAGGGCC TCCAGGCAGT CCTGGCCTCC CTGGAGTTCC TGGGTCTCCT 
GGCATTAAGG GCTCCACGGG GCGGCCTGGC CCTCGTGGGG AACAGGGAGA ACGAGGGCCT 
CAAGGTCCAA AGGGGGAGCC GGGTGAGCCT GGGCAAATCA CTGGCGGTGG AGGGCCAGGG 
TTTCCTGGAA AGAAAGGGGA CCCTGGACCT TCGGGGCCCC CTGGATCTCG TGGCCCTGTG 
GGGGACCCAG GACCCCGTGG CCCCCCAGGG CTTCCTGGAA TATCAGTGAA GGGTGACAAA 
GGTGATCGTG GGGAACGGGG TCCCCCGGGA CCAGGCATTG GTGCCAGTGA ACAGGGTGAC 
CCTGGACTTC CGGGGCTTCC TGGAAGCCCT GGACCCCAAG GCCCAGCTGG CCGACCTGGA 
GAGAAGGGAG AAAAGGGTGA CTGTGAGGAT GGAGGTCCAG GCCTCCCAGG ACAGCCTGGG 
CCCCCAGGGG AGCCGGGCCT GCGGGGAGCT CCTGGAATGA CTGGGCCCAA AGGTGATCGG 
GGACTGACGG GGACCCCGGG CGAGCCTGGA GTAAAGGGTG AACGGGGACA TCCTGGTCCC 
GTGGGACCCC AGGGGCTCCC CGGGGCTGCT GGACACCCTG GAGTGGAGGG TCCTGAGGGG 
CCACCAGGAC CCACTGGCCG CCGAGGAGAA AAGGGAGAAC CTGGTCGCCC TGGGGATCCT 
GCAGTGGGTC CTGGTGGTGC TGGAGCCAAA GGAGAAAAGG GAGAGGCTGG GCTCCCTGGG 
CCCAGAGGAG CTTCTGGAAG CAAAGGAGAG CAGGGCGCAC CTGGATTGGC TCTTCCTGGA 
GATCCTGGCC CCAAAGGAGA CCCTGGAGAC AGGGGTCCCA TTGGCCTCAC TGGAAGGGCA 
GGACCCACAG GTGACTCAGG GCCTCCTGGA GAGAAAGGAG AGCCTGGTCG ACCTGGTTCC 
CCAGGACCTG TTGGCCCCCG AGGAAGAGAT GGTGAAGCTG GAGAGAAAGG TGACGAGGGG 
ATCCCGGGTG AGCCAGGTTT GCCTGGAAAA GCAGGTGAAC GTGGCCTCCG GGGGGCACCT 
GGGCCTCGGG GGCCTGTGGG TGAGAAGGGG GACCAGGGAG ATCCTGGAGA AGACGGACGG 
AATGGCAGCC CTGGATCATC TGGACCCAAG GGTGACCGTG GGGAGCCTGG TCCCCCAGGT 
CCCCCTGGAC GGCTGGTGGA TGCAGGCATT GAATCCAGAG ACAAGGGAGA GCCTGGACAA 
GAAGGTCCCC GAGGACCCAA GGGTGACCCT GGGCCCCCTG GAGTCTCTGG AGAAAGGGGC 
ATTGATGGGC TTCGGGGACC CCCAGGCCCA CAGGGAGACC CCGGTGTTCG AGGCCCAGCA 
GGAGACAAGG GTGATCGGGG ACCCCCAGGG CTGGATGGCC GGAGTGGGCT GGATGGGAAA 
CCCGGAGCCC CTGGCCCCCC AGGGCTACAT GGTGCTTCAG GCAAAGCTGG GGACCCGGGG 
AGAGATGGAC TTCCAGGCCT TCGAGGAGAA CATGGCCCCC CTGGTCCCCC TGGCCCTCCT 
GGAGTTCCGG GAAAGGCAGG CGATGATGGC AAACCAGGCC TGAATGGGAA AAACGGAGAC 
CCTGGAGACC CTGGAGAAGA TGGAAGGAAG GGAGAAAAGG GAGATTCGGG TGCCCCTGGA 
AGAGAAGGCC CTGATGGCCC CAAGGGTGAA CGTGGAGCTC CTGGCAATCC TGGACTCCAG 
GGTCCTCCGG GTCTCCCAGG GCAGGTCGGT CCTCCCGGCC AGGGTTTCCC TGGTGTCCCA 
GGAATTACGG GTCCTAAGGG TGACCGTGGA GAGACTGGAT CCAAAGGGGA ACAGGGCCTT 
CCTGGAGAAC GTGGCCTACG AGGAGAACCT GGGAGCTTGC CAAACGCGGA GCGTTTGCTG 
GAAACTGCTG GCATCAAGGT GTCGGCCCTG CGGGAGATCG TGGACACCTG GGATGAGAGT 
TCTGGCAGCT TCCTACCGGT GCCTGAGCGG AGACCTGGCC CCAAGGGGGA CCCTGGTGAC 
CGAGGCCCTC CAGGCAAGGA GGGCCTCATC GGCTTTCCTG GAGAACGTGG ACTGAAGGGA 
GAGCGTGGAG ACCCTGGCCC TCAGGGGCCT CCTGGCCTGG CCCTGGGTGA GAGGGGCCCA 
CCTGGCCCAC CCGGCCTTGC GGGGGAACCC GGAAAGCCTG GCATTCCTGG ACTCCCAGGC 
CGGGCTGGTG GTTCAGGGGA AGCAGGAAGG CCAGGAGAGA GGGGAGAAAG GGGAGAGAAA 
GGAGAACGCG GGGATCAGGG CAGAGATGGC CTTCCCGGCC TCCCTGGACC ACCTGGCCCT 
CCTGGCCCCA AGGTGGCCAT TGAAGAGCCA GGCCCTGGAC TGGCTAGAGA GCAAGGACCT 
CCTGGACTCA AGGGTGCCAA GGGGGAGCCA GGCAGTGATG GTGACCCTGG ACCTAAAGGA 
GACAGGGGTG TACCAGGCAT CAAAGGCGAT GTGGGAGAGC CTGGAAAGAG GGGTCATGAT 
GGCAACCCAG GTCTCCCGGG AGAGCGTGGT GTGGCTGGGC CTGAAGGGAA GCCGGGTCTT 
CAGGGCCCAA GGGGGACCCC TGGCCCAGTG GGTAGCCATG GAGACCCTGG ACCACCTGGT 
GCCCCGGGTC TTGCTGGCCC TGCGGGACCC CAGGGGCCTT CTGGCCTGAA GGGGGAGCCT 
GGAGAGACAG GACCTCCAGG ACGGGGTCTG CCTGGACCTG TTGGAGCTGT GGGACTCCCT 
GGCCCTCCTG GCCCTTCCGG CCTCGTGGGT CCACAAGGCT CTCCAGGTTT GCCCGGACAA 
GTGGGGGAGA CAGGGAAGCC AGGACCTCCA GGCCGTGACG GTAGCAGTGG AAAGGACGGA 
GATCGGGGAA GTCCTGGTGT GCCGGGGTCA CCAGGTCTGC CCGGCCCTGT TGGACCTAAA 
GGAGAACCTG GGCCTGTGGG GGCTCCTGGA CAGGTGGTGG TCGGGCCCCC TGGAGCAAAA 
GGCGAGAAGG GAGCCCCGGG AGACCTTGCT GGAGCCCTGC TGGGTGAGCC GGGAGCCAAA 
GGTGACCGAG GACTGCCTGG ACCACGGGGT GAAAAGGGTG AAGCTGGCCG TGCAGGAGGA 
CCTGGGGACC CTGGGGAAGA TGGTCAAAAA GGGGCTCCAG GACTCAAAGG TCTTAAGGGT 
GAGCCAGGCA TTGGGGTTCA GGGCCCCCCT GGCCCAAGTG GTCCTCCAGG TATGAAGGGA 
GACTTGGGCC CCCCTGGTGC CCCCGGGGCT CCTGGTGTTG TTGGGTTCCC TGGTCAGACA 
GGACCTCGAG GAGAGACGGG CCAGCCAGGG CCTGTTGGAG AGAGGGGTCT GGCAGGCCCT 
CCAGGGAGAG AAGGTGCCCC AGGTCCCTTG GGACCACCTG GACCACCAGG GTCAGCGGGA 
GCACCTGGCG CCTCTGGACT CAAAGGAGAC AAGGGAGACC CGGGAGCAGG ACTGCCGGGG 
CCCCGAGGGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Impaired lymphoid extracellular matrix impedes antibacterial immunity in epidermolysis bullosa.
Proceedings of the National Academy of Sciences of the United States of America(2018 Jan)
Nystr?m A,Bornert O,K?hl T,Gretzmeier C,Thriene K,Dengjel J,Pfister-Wartha A,Kiritsi D,Bruckner-Tuderman L

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Col7a1 cDNA ORF clone, Mus musculus(house mouse)

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Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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