Col4a1 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Col4a1
Entrez Gene ID	12826
Full Name	collagen, type IV, alpha 1
Synonyms	Bru,Col4a-1,Raw,Svc
General protein information	Preferred Names collagen, type IV, alpha 1 Names collagen alpha-1(IV) chain alpha-1 type IV collagen alpha1(IV) collagen procollagen, type IV, alpha 1
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 8 Map Location: 8 A1.1\|8 5.53 cM
Summary	This gene encodes the alpha-1 subunit of the type IV collagens, an essential component of basement membranes. The encoded protein forms a triple helical heterotrimer comprised of two alpha-1 and one alpha-2 subunits that assembles into a type IV collagen network. This gene is located adjacent to the gene encoding alpha-2 subunit. Mice lacking both the alpha-1 and alpha-2 subunits of collagen IV die in utero due to structural deficiencies in the basement membranes and certain mutations in this gene cause perinatal cerebral hemorrhage and porencephaly. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq, Nov 2015].

mRNA and Protein(s)

mRNA	Protein	Name
XM_017312555.1	XP_017168044.1	collagen alpha-1(IV) chain isoform X1
XM_017312555.2	XP_017168044.1	collagen alpha-1(IV) chain isoform X1
NM_009931.2	NP_034061.2	collagen alpha-1(IV) chain precursor

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	COL4A1	XP_003314261.2
Mus musculus (house mouse)	Col4a1	NP_034061.2
Rattus norvegicus (Norway rat)	Col4a1	NP_001128481.1
Xenopus tropicalis (tropical clawed frog)	col4a1	XP_002933064.2
	COL4A1	XP_001088660.2
Canis lupus familiaris (dog)	COL4A1	XP_005634201.1
Danio rerio (zebrafish)	col4a1	XP_694040.5
Gallus gallus (chicken)	COL4A1	NP_001155871.1
Homo sapiens (human)	COL4A1	NP_001836.2
Bos taurus (cattle)	COL4A1	NP_001159983.1

Gene Ontology

The following Col4a1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Col4a1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu82340	XM_017312555.2 Latest version!	Mus musculus collagen, type IV, alpha 1 (Col4a1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OMu21933	NM_009931.2 Latest version!	Mus musculus collagen, type IV, alpha 1 (Col4a1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$951.30 $1359.00
View Old Accession Versions >>
OMu82340	XM_017312555.1	Mus musculus collagen, type IV, alpha 1 (Col4a1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$811.30 $1159.00
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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Col4a1 ( XM_017312555.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017168044.1 Mus musculus collagen, type IV, alpha 1 (Col4a1), transcript variant X1, mRNA.

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CloneID	OMu82340
Clone ID Related Accession (Same CDS sequence)	XM_017312555.1 , XM_017312555.2
Accession Version	XM_017312555.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4818bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	collagen alpha-1(IV) chain isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000074.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_017312555.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGCCCC GGCTCAGCGT CTGGCTTCTG CTGCTCTTCG CCGCCCTTCT GCTCCACGAG 
GAGCGCAGCC GAGCAGCTGC GAAGGGCGAT TGTGGTGGCT CTGGCTGTGG AAAATGTGAC 
TGTCATGGCG TGAAGGGACA AAAGGGAGAA AGAGGCTTGC CAGGGTTGCA AGGTGTCATT 
GGATTTCCCG GGATGCAAGG ACCTGAGGGG CCACATGGAC CACCAGGACA AAAGGGTGAT 
GCTGGAGAAC CTGGACTTCC TGGCACAAAA GGGACGAGGG GACCCCCTGG AGCAGCTGGC 
TACCCTGGGA ATCCCGGACT TCCTGGTATT CCTGGCCAAG ATGGCCCTCC AGGTCCCCCA 
GGTATCCCAG GATGCAACGG TACAAAGGGA GAGAGAGGGC CGCTCGGTCC TCCTGGCTTG 
CCTGGATTCA GCGGCAATCC TGGACCACCA GGGTTACCAG GAATGAAGGG AGATCCTGGT 
GAAATTCTCG GCCACGTTCC CGGAACACTG CTGAAAGGGG AGAGAGGATT TCCTGGTATC 
CCCGGGATGC CGGGCTCACC AGGGTTGCCA GGACTCCAGG GACCCGTGGG TCCTCCGGGA 
TTTACTGGAC CACCGGGTCC TCCAGGCCCT CCTGGACCTC CTGGAGAAAA GGGCCAGATG 
GGCTCCAGCT TCCAAGGACC AAAAGGTGAC AAGGGAGAGC AAGGGGTCAG CGGTCCCCCG 
GGAGTTCCTG GACAGGCACA AGTTAAGGAA AAAGGAGACT TTGCCCCAAC AGGAGAAAAG 
GGTCAGAAAG GTGAACCTGG ATTTCCGGGA GTACCAGGAT ATGGAGAGAA AGGTGAACCT 
GGCAAGCAAG GGCCTCGGGG AAAACCTGGG AAAGACGGTG AAAAAGGAGA AAGGGGGAGC 
CCGGGCATTC CTGGCGATTC CGGGTACCCA GGTCTCCCAG GCCGGCAGGG CCCGCAGGGA 
GAGAAGGGTG AAGCTGGACT TCCAGGCCCC CCTGGAACTG TGATAGGCAC CATGCCTTTG 
GGAGAGAAAG GAGATCGAGG CTACCCAGGA GCACCAGGGT TGAGAGGAGA GCCCGGCCCT 
AAAGGTTTCC CTGGAACACC AGGCCAACCA GGCCCTCCAG GTTTCCCTAC TCCAGGCCAG 
GCTGGTGCTC CAGGCTTCCC AGGTGAAAGG GGAGAAAAAG GTGACCAGGG ATTTCCAGGC 
GTGTCATTGC CAGGACCAAG TGGAAGAGAT GGAGCCCCGG GGCCTCCCGG CCCTCCCGGC 
CCCCCTGGGC AGCCAGGCCA CACAAATGGC ATTGTGGAGT GTCAACCTGG ACCACCCGGG 
GACCAGGGCC CTCCTGGGAC TCCAGGACAG CCAGGTTTGA CAGGTGAAGT TGGACAGAAA 
GGTCAGAAAG GAGAGAGCTG CCTTGCCTGT GACACAGAAG GACTTCGTGG CCCTCCAGGG 
CCACAGGGGC CTCCGGGAGA GATTGGTTTC CCTGGACAAC CTGGGGCTAA AGGTGACCGA 
GGCCTGCCCG GCAGAGATGG TCTTGAAGGA TTGCCGGGTC CACAAGGTTC ACCAGGGCTT 
ATAGGCCAGC CTGGAGCTAA GGGAGAGCCT GGAGAGATAT TTTTTGACAT GCGACTCAAA 
GGTGACAAAG GAGACCCAGG TTTTCCGGGA CAGCCTGGCA TGCCAGGAAG AGCAGGAACT 
CCCGGAAGAG ATGGCCACCC AGGACTCCCT GGACCCAAAG GCTCTCCGGG TTCAATAGGA 
TTGAAAGGAG AGCGAGGTCC TCCCGGAGGA GTTGGCTTCC CTGGTAGTCG TGGAGACATC 
GGCCCTCCTG GACCCCCAGG AGTTGGCCCC ATTGGTCCTG TTGGTGAAAA AGGACAAGCA 
GGCTTTCCTG GAGGCCCTGG GTCCCCAGGT CTTCCAGGCC CAAAGGGTGA GGCAGGAAAG 
GTTGTCCCAC TCCCTGGCCC CCCAGGAGCT GCAGGACTTC CAGGATCCCC TGGCTTCCCA 
GGGCCACAAG GTGACCGAGG CTTCCCAGGA ACCCCAGGAC GTCCAGGCAT CCCGGGAGAG 
AAAGGTGCTG TGGGCCAGCC AGGAATTGGA TTTCCTGGGC TTCCTGGCCC CAAAGGTGTT 
GATGGCTTGC CTGGAGAGAT AGGACGGCCT GGGAGTCCAG GTCGCCCTGG ATTTAACGGC 
TTACCTGGCA ACCCAGGACC TCAAGGTCAA AAGGGAGAAC CTGGCATTGG GCTTCCAGGA 
CTCAAAGGGC AACCAGGCCT TCCAGGCATT CCCGGTACAC CTGGAGAGAA GGGTAGCATC 
GGGGGACCCG GCGTTCCAGG AGAACAGGGG TTGACAGGCC CCCCAGGACT CCAGGGGATC 
AGAGGTGACC CAGGGCCTCC TGGAGTTCAA GGCCCAGCAG GTCCACCAGG GGTCCCAGGA 
ATAGGGCCAC CCGGAGCTAT GGGCCCTCCC GGAGGGCAAG GACCACCAGG GTCATCAGGT 
CCACCTGGAA TTAAAGGAGA GAAAGGGTTC CCTGGATTCC CCGGACTGGA TATGCCTGGC 
CCCAAAGGCG ATAAAGGCTC TCAAGGACTT CCTGGCCTCA CAGGACAGTC AGGCCTCCCT 
GGCCTTCCTG GACAGCAGGG GACACCTGGA GTTCCAGGGT TCCCAGGTTC TAAAGGTGAA 
ATGGGTGTCA TGGGAACCCC GGGACAACCA GGCTCGCCAG GACCAGCAGG CACCCCAGGG 
TTACCTGGAG AAAAAGGGGA CCATGGCCTT CCGGGCTCCT CAGGACCCAG GGGCGACCCT 
GGCTTCAAAG GTGATAAAGG TGACGTTGGG CTTCCTGGCA TGCCAGGATC CATGGAGCAT 
GTGGACATGG GGAGCATGAA GGGACAGAAA GGAGACCAGG GAGAGAAAGG ACAAATCGGA 
CCCACTGGTG ATAAAGGTTC CCGAGGAGAC CCTGGAACAC CAGGAGTACC TGGGAAGGAT 
GGGCAGGCAG GGCATCCCGG ACAGCCAGGG CCTAAAGGTG ACCCAGGCCT TAGTGGGACA 
CCAGGATCCC CTGGACTCCC TGGACCCAAA GGATCAGTTG GAGGAATGGG CTTGCCAGGT 
TCGCCTGGAG AAAAAGGTGT GCCTGGCATC CCTGGCTCAC AGGGTGTCCC TGGCTCACCT 
GGAGAGAAGG GAGCCAAAGG AGAGAAAGGG CAGTCAGGTC TACCTGGCAT TGGGATTCCG 
GGACGGCCTG GTGACAAGGG AGACCAGGGC CTTGCTGGCT TCCCAGGCAG CCCCGGTGAG 
AAGGGAGAGA AAGGCAGTGC CGGAACCCCA GGGATGCCAG GGTCCCCAGG CCCGAGAGGT 
TCTCCGGGGA ACATCGGCCA TCCAGGAAGC CCAGGCCTGC CTGGAGAGAA AGGGGATAAA 
GGCCTCCCAG GACTGGATGG CGTTCCTGGT GTCAAAGGAG AAGCAGGTCT CCCTGGGACT 
CCTGGCCCCA CAGGCCCAGC TGGCCAGAAG GGAGAGCCGG GCAGCGATGG AATCCCGGGG 
TCGGCAGGAG AGAAGGGTGA ACAAGGTGTT CCAGGAAGAG GCTTCCCCGG CTTCCCTGGC 
TCCAAAGGAG ACAAAGGCTC CAAGGGTGAA GTGGGTTTCC CTGGCCTAGC TGGAAGTCCT 
GGAATTCCTG GAGTCAAAGG CGAGCAAGGG TTCATGGGTC CTCCTGGCCC TCAAGGACAA 
CCTGGCTTAC CTGGCACTCC TGGTCACCCT GTGGAGGGGC CCAAAGGAGA CCGAGGACCT 
CAGGGTCAAC CTGGCCTGCC AGGGCATCCG GGACCTATGG GGCCGCCAGG GTTCCCAGGA 
ATCAATGGGC CAAAAGGTGA CAAGGGAAAT CAAGGTTGGC CAGGAGCTCC GGGGGTTCCA 
GGCCCTAAGG GAGACCCAGG ATTCCAAGGC ATGCCGGGCA TTGGCGGCTC TCCAGGGATC 
ACAGGTTCAA AGGGAGATAT GGGACTGCCC GGAGTTCCAG GATTTCAAGG TCAGAAAGGG 
CTTCCTGGTC TGCAGGGAGT GAAAGGAGAC CAGGGAGACC AAGGTGTACC CGGCCCTAAA 
GGTCTCCAAG GTCCCCCTGG GCCTCCAGGT CCCTACGATG TCATCAAAGG AGAACCAGGG 
CTCCCTGGTC CTGAGGGTCC CCCTGGTCTT AAAGGACTCC AGGGACCACC AGGTCCAAAA 
GGACAGCAAG GTGTGACAGG CTCAGTGGGC TTGCCTGGAC CTCCAGGTGT CCCTGGGTTC 
GATGGTGCTC CTGGCCAGAA AGGAGAGACA GGACCCTTTG GACCACCTGG TCCAAGAGGG 
TTTCCTGGCC CACCAGGCCC CGATGGGCTG CCAGGATCCA TGGGTCCCCC AGGTACCCCA 
TCTGTGGACC ATGGCTTCCT TGTGACCAGG CATAGTCAGA CAACAGATGA CCCACTGTGT 
CCCCCAGGGA CCAAAATTCT TTACCATGGA TACTCTCTGC TCTATGTCCA AGGCAACGAG 
CGTGCCCACG GGCAGGACTT GGGTACGGCT GGCAGCTGCC TGCGTAAGTT CAGCACCATG 
CCCTTTCTCT TCTGCAACAT CAACAACGTC TGCAACTTCG CCTCCAGGAA CGACTACTCT 
TACTGGCTGT CCACGCCAGA GCCCATGCCC ATGTCCATGG CACCCATCTC TGGGGACAAC 
ATCCGGCCCT TCATTAGCAG CTTTCCTTGG AAAACAGGTA GAAAATGTTT ATTATTGATG 
TCTCTTGGTT ACTTTTGGAA TGGCGTCTAT GCTGGTCTTG ACTTGGTTGA AGACACAGGC 
CCAGGGAAAC TGTGTGTCTC ATTCTTTTCT CCATGGGCAT TTGTCTTCAC AAAGTTCCTG 
TTCTGTGAGC ACACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017168044.1
CDS	291..5108
Translation	MGPRLSVWLLLLFAALLLHEERSRAAAKGDCGGSGCGKCDCHGVKGQKGERGLPGLQGVIGFPGMQGPEGPHGPPGQKGDAGEPGLPGTKGTRGPPGAAGYPGNPGLPGIPGQDGPPGPPGIPGCNGTKGERGPLGPPGLPGFSGNPGPPGLPGMKGDPGEILGHVPGTLLKGERGFPGIPGMPGSPGLPGLQGPVGPPGFTGPPGPPGPPGPPGEKGQMGSSFQGPKGDKGEQGVSGPPGVPGQAQVKEKGDFAPTGEKGQKGEPGFPGVPGYGEKGEPGKQGPRGKPGKDGEKGERGSPGIPGDSGYPGLPGRQGPQGEKGEAGLPGPPGTVIGTMPLGEKGDRGYPGAPGLRGEPGPKGFPGTPGQPGPPGFPTPGQAGAPGFPGERGEKGDQGFPGVSLPGPSGRDGAPGPPGPPGPPGQPGHTNGIVECQPGPPGDQGPPGTPGQPGLTGEVGQKGQKGESCLACDTEGLRGPPGPQGPPGEIGFPGQPGAKGDRGLPGRDGLEGLPGPQGSPGLIGQPGAKGEPGEIFFDMRLKGDKGDPGFPGQPGMPGRAGTPGRDGHPGLPGPKGSPGSIGLKGERGPPGGVGFPGSRGDIGPPGPPGVGPIGPVGEKGQAGFPGGPGSPGLPGPKGEAGKVVPLPGPPGAAGLPGSPGFPGPQGDRGFPGTPGRPGIPGEKGAVGQPGIGFPGLPGPKGVDGLPGEIGRPGSPGRPGFNGLPGNPGPQGQKGEPGIGLPGLKGQPGLPGIPGTPGEKGSIGGPGVPGEQGLTGPPGLQGIRGDPGPPGVQGPAGPPGVPGIGPPGAMGPPGGQGPPGSSGPPGIKGEKGFPGFPGLDMPGPKGDKGSQGLPGLTGQSGLPGLPGQQGTPGVPGFPGSKGEMGVMGTPGQPGSPGPAGTPGLPGEKGDHGLPGSSGPRGDPGFKGDKGDVGLPGMPGSMEHVDMGSMKGQKGDQGEKGQIGPTGDKGSRGDPGTPGVPGKDGQAGHPGQPGPKGDPGLSGTPGSPGLPGPKGSVGGMGLPGSPGEKGVPGIPGSQGVPGSPGEKGAKGEKGQSGLPGIGIPGRPGDKGDQGLAGFPGSPGEKGEKGSAGTPGMPGSPGPRGSPGNIGHPGSPGLPGEKGDKGLPGLDGVPGVKGEAGLPGTPGPTGPAGQKGEPGSDGIPGSAGEKGEQGVPGRGFPGFPGSKGDKGSKGEVGFPGLAGSPGIPGVKGEQGFMGPPGPQGQPGLPGTPGHPVEGPKGDRGPQGQPGLPGHPGPMGPPGFPGINGPKGDKGNQGWPGAPGVPGPKGDPGFQGMPGIGGSPGITGSKGDMGLPGVPGFQGQKGLPGLQGVKGDQGDQGVPGPKGLQGPPGPPGPYDVIKGEPGLPGPEGPPGLKGLQGPPGPKGQQGVTGSVGLPGPPGVPGFDGAPGQKGETGPFGPPGPRGFPGPPGPDGLPGSMGPPGTPSVDHGFLVTRHSQTTDDPLCPPGTKILYHGYSLLYVQGNERAHGQDLGTAGSCLRKFSTMPFLFCNINNVCNFASRNDYSYWLSTPEPMPMSMAPISGDNIRPFISSFPWKTGRKCLLLMSLGYFWNGVYAGLDLVEDTGPGKLCVSFFSPWAFVFTKFLFCEHT

Target ORF information:

RefSeq Version	XM_017312555.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus collagen, type IV, alpha 1 (Col4a1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017312555.2

ORF Insert Sequence:

ATGGGGCCCC GGCTCAGCGT CTGGCTTCTG CTGCTCTTCG CCGCCCTTCT GCTCCACGAG 
GAGCGCAGCC GAGCAGCTGC GAAGGGCGAT TGTGGTGGCT CTGGCTGTGG AAAATGTGAC 
TGTCATGGCG TGAAGGGACA AAAGGGAGAA AGAGGCTTGC CAGGGTTGCA AGGTGTCATT 
GGATTTCCCG GGATGCAAGG ACCTGAGGGG CCACATGGAC CACCAGGACA AAAGGGTGAT 
GCTGGAGAAC CTGGACTTCC TGGCACAAAA GGGACGAGGG GACCCCCTGG AGCAGCTGGC 
TACCCTGGGA ATCCCGGACT TCCTGGTATT CCTGGCCAAG ATGGCCCTCC AGGTCCCCCA 
GGTATCCCAG GATGCAACGG TACAAAGGGA GAGAGAGGGC CGCTCGGTCC TCCTGGCTTG 
CCTGGATTCA GCGGCAATCC TGGACCACCA GGGTTACCAG GAATGAAGGG AGATCCTGGT 
GAAATTCTCG GCCACGTTCC CGGAACACTG CTGAAAGGGG AGAGAGGATT TCCTGGTATC 
CCCGGGATGC CGGGCTCACC AGGGTTGCCA GGACTCCAGG GACCCGTGGG TCCTCCGGGA 
TTTACTGGAC CACCGGGTCC TCCAGGCCCT CCTGGACCTC CTGGAGAAAA GGGCCAGATG 
GGCTCCAGCT TCCAAGGACC AAAAGGTGAC AAGGGAGAGC AAGGGGTCAG CGGTCCCCCG 
GGAGTTCCTG GACAGGCACA AGTTAAGGAA AAAGGAGACT TTGCCCCAAC AGGAGAAAAG 
GGTCAGAAAG GTGAACCTGG ATTTCCGGGA GTACCAGGAT ATGGAGAGAA AGGTGAACCT 
GGCAAGCAAG GGCCTCGGGG AAAACCTGGG AAAGACGGTG AAAAAGGAGA AAGGGGGAGC 
CCGGGCATTC CTGGCGATTC CGGGTACCCA GGTCTCCCAG GCCGGCAGGG CCCGCAGGGA 
GAGAAGGGTG AAGCTGGACT TCCAGGCCCC CCTGGAACTG TGATAGGCAC CATGCCTTTG 
GGAGAGAAAG GAGATCGAGG CTACCCAGGA GCACCAGGGT TGAGAGGAGA GCCCGGCCCT 
AAAGGTTTCC CTGGAACACC AGGCCAACCA GGCCCTCCAG GTTTCCCTAC TCCAGGCCAG 
GCTGGTGCTC CAGGCTTCCC AGGTGAAAGG GGAGAAAAAG GTGACCAGGG ATTTCCAGGC 
GTGTCATTGC CAGGACCAAG TGGAAGAGAT GGAGCCCCGG GGCCTCCCGG CCCTCCCGGC 
CCCCCTGGGC AGCCAGGCCA CACAAATGGC ATTGTGGAGT GTCAACCTGG ACCACCCGGG 
GACCAGGGCC CTCCTGGGAC TCCAGGACAG CCAGGTTTGA CAGGTGAAGT TGGACAGAAA 
GGTCAGAAAG GAGAGAGCTG CCTTGCCTGT GACACAGAAG GACTTCGTGG CCCTCCAGGG 
CCACAGGGGC CTCCGGGAGA GATTGGTTTC CCTGGACAAC CTGGGGCTAA AGGTGACCGA 
GGCCTGCCCG GCAGAGATGG TCTTGAAGGA TTGCCGGGTC CACAAGGTTC ACCAGGGCTT 
ATAGGCCAGC CTGGAGCTAA GGGAGAGCCT GGAGAGATAT TTTTTGACAT GCGACTCAAA 
GGTGACAAAG GAGACCCAGG TTTTCCGGGA CAGCCTGGCA TGCCAGGAAG AGCAGGAACT 
CCCGGAAGAG ATGGCCACCC AGGACTCCCT GGACCCAAAG GCTCTCCGGG TTCAATAGGA 
TTGAAAGGAG AGCGAGGTCC TCCCGGAGGA GTTGGCTTCC CTGGTAGTCG TGGAGACATC 
GGCCCTCCTG GACCCCCAGG AGTTGGCCCC ATTGGTCCTG TTGGTGAAAA AGGACAAGCA 
GGCTTTCCTG GAGGCCCTGG GTCCCCAGGT CTTCCAGGCC CAAAGGGTGA GGCAGGAAAG 
GTTGTCCCAC TCCCTGGCCC CCCAGGAGCT GCAGGACTTC CAGGATCCCC TGGCTTCCCA 
GGGCCACAAG GTGACCGAGG CTTCCCAGGA ACCCCAGGAC GTCCAGGCAT CCCGGGAGAG 
AAAGGTGCTG TGGGCCAGCC AGGAATTGGA TTTCCTGGGC TTCCTGGCCC CAAAGGTGTT 
GATGGCTTGC CTGGAGAGAT AGGACGGCCT GGGAGTCCAG GTCGCCCTGG ATTTAACGGC 
TTACCTGGCA ACCCAGGACC TCAAGGTCAA AAGGGAGAAC CTGGCATTGG GCTTCCAGGA 
CTCAAAGGGC AACCAGGCCT TCCAGGCATT CCCGGTACAC CTGGAGAGAA GGGTAGCATC 
GGGGGACCCG GCGTTCCAGG AGAACAGGGG TTGACAGGCC CCCCAGGACT CCAGGGGATC 
AGAGGTGACC CAGGGCCTCC TGGAGTTCAA GGCCCAGCAG GTCCACCAGG GGTCCCAGGA 
ATAGGGCCAC CCGGAGCTAT GGGCCCTCCC GGAGGGCAAG GACCACCAGG GTCATCAGGT 
CCACCTGGAA TTAAAGGAGA GAAAGGGTTC CCTGGATTCC CCGGACTGGA TATGCCTGGC 
CCCAAAGGCG ATAAAGGCTC TCAAGGACTT CCTGGCCTCA CAGGACAGTC AGGCCTCCCT 
GGCCTTCCTG GACAGCAGGG GACACCTGGA GTTCCAGGGT TCCCAGGTTC TAAAGGTGAA 
ATGGGTGTCA TGGGAACCCC GGGACAACCA GGCTCGCCAG GACCAGCAGG CACCCCAGGG 
TTACCTGGAG AAAAAGGGGA CCATGGCCTT CCGGGCTCCT CAGGACCCAG GGGCGACCCT 
GGCTTCAAAG GTGATAAAGG TGACGTTGGG CTTCCTGGCA TGCCAGGATC CATGGAGCAT 
GTGGACATGG GGAGCATGAA GGGACAGAAA GGAGACCAGG GAGAGAAAGG ACAAATCGGA 
CCCACTGGTG ATAAAGGTTC CCGAGGAGAC CCTGGAACAC CAGGAGTACC TGGGAAGGAT 
GGGCAGGCAG GGCATCCCGG ACAGCCAGGG CCTAAAGGTG ACCCAGGCCT TAGTGGGACA 
CCAGGATCCC CTGGACTCCC TGGACCCAAA GGATCAGTTG GAGGAATGGG CTTGCCAGGT 
TCGCCTGGAG AAAAAGGTGT GCCTGGCATC CCTGGCTCAC AGGGTGTCCC TGGCTCACCT 
GGAGAGAAGG GAGCCAAAGG AGAGAAAGGG CAGTCAGGTC TACCTGGCAT TGGGATTCCG 
GGACGGCCTG GTGACAAGGG AGACCAGGGC CTTGCTGGCT TCCCAGGCAG CCCCGGTGAG 
AAGGGAGAGA AAGGCAGTGC CGGAACCCCA GGGATGCCAG GGTCCCCAGG CCCGAGAGGT 
TCTCCGGGGA ACATCGGCCA TCCAGGAAGC CCAGGCCTGC CTGGAGAGAA AGGGGATAAA 
GGCCTCCCAG GACTGGATGG CGTTCCTGGT GTCAAAGGAG AAGCAGGTCT CCCTGGGACT 
CCTGGCCCCA CAGGCCCAGC TGGCCAGAAG GGAGAGCCGG GCAGCGATGG AATCCCGGGG 
TCGGCAGGAG AGAAGGGTGA ACAAGGTGTT CCAGGAAGAG GCTTCCCCGG CTTCCCTGGC 
TCCAAAGGAG ACAAAGGCTC CAAGGGTGAA GTGGGTTTCC CTGGCCTAGC TGGAAGTCCT 
GGAATTCCTG GAGTCAAAGG CGAGCAAGGG TTCATGGGTC CTCCTGGCCC TCAAGGACAA 
CCTGGCTTAC CTGGCACTCC TGGTCACCCT GTGGAGGGGC CCAAAGGAGA CCGAGGACCT 
CAGGGTCAAC CTGGCCTGCC AGGGCATCCG GGACCTATGG GGCCGCCAGG GTTCCCAGGA 
ATCAATGGGC CAAAAGGTGA CAAGGGAAAT CAAGGTTGGC CAGGAGCTCC GGGGGTTCCA 
GGCCCTAAGG GAGACCCAGG ATTCCAAGGC ATGCCGGGCA TTGGCGGCTC TCCAGGGATC 
ACAGGTTCAA AGGGAGATAT GGGACTGCCC GGAGTTCCAG GATTTCAAGG TCAGAAAGGG 
CTTCCTGGTC TGCAGGGAGT GAAAGGAGAC CAGGGAGACC AAGGTGTACC CGGCCCTAAA 
GGTCTCCAAG GTCCCCCTGG GCCTCCAGGT CCCTACGATG TCATCAAAGG AGAACCAGGG 
CTCCCTGGTC CTGAGGGTCC CCCTGGTCTT AAAGGACTCC AGGGACCACC AGGTCCAAAA 
GGACAGCAAG GTGTGACAGG CTCAGTGGGC TTGCCTGGAC CTCCAGGTGT CCCTGGGTTC 
GATGGTGCTC CTGGCCAGAA AGGAGAGACA GGACCCTTTG GACCACCTGG TCCAAGAGGG 
TTTCCTGGCC CACCAGGCCC CGATGGGCTG CCAGGATCCA TGGGTCCCCC AGGTACCCCA 
TCTGTGGACC ATGGCTTCCT TGTGACCAGG CATAGTCAGA CAACAGATGA CCCACTGTGT 
CCCCCAGGGA CCAAAATTCT TTACCATGGA TACTCTCTGC TCTATGTCCA AGGCAACGAG 
CGTGCCCACG GGCAGGACTT GGGTACGGCT GGCAGCTGCC TGCGTAAGTT CAGCACCATG 
CCCTTTCTCT TCTGCAACAT CAACAACGTC TGCAACTTCG CCTCCAGGAA CGACTACTCT 
TACTGGCTGT CCACGCCAGA GCCCATGCCC ATGTCCATGG CACCCATCTC TGGGGACAAC 
ATCCGGCCCT TCATTAGCAG CTTTCCTTGG AAAACAGGTA GAAAATGTTT ATTATTGATG 
TCTCTTGGTT ACTTTTGGAA TGGCGTCTAT GCTGGTCTTG ACTTGGTTGA AGACACAGGC 
CCAGGGAAAC TGTGTGTCTC ATTCTTTTCT CCATGGGCAT TTGTCTTCAC AAAGTTCCTG 
TTCTGTGAGC ACACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Col4a1 ( NM_009931.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_034061.2 Mus musculus collagen, type IV, alpha 1 (Col4a1), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OMu21933
Clone ID Related Accession (Same CDS sequence)	NM_009931.2
Accession Version	NM_009931.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5010bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-25
Organism	Mus musculus(house mouse)
Product	collagen alpha-1(IV) chain precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC114598.9, CJ108618.1, AK147661.1, AC115800.13 and CJ248638.1. On Dec 1, 2007 this sequence version replaced NM_009931.1. Transcript Variant: This variant (1) represents the shorter transcript and encodes the functional protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK147661.1, AK147355.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849381 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: full length.

ATGGGGCCCC GGCTCAGCGT CTGGCTTCTG CTGCTCTTCG CCGCCCTTCT GCTCCACGAG 
GAGCGCAGCC GAGCAGCTGC GAAGGGCGAT TGTGGTGGCT CTGGCTGTGG AAAATGTGAC 
TGTCATGGCG TGAAGGGACA AAAGGGAGAA AGAGGCTTGC CAGGGTTGCA AGGTGTCATT 
GGATTTCCCG GGATGCAAGG ACCTGAGGGG CCACATGGAC CACCAGGACA AAAGGGTGAT 
GCTGGAGAAC CTGGACTTCC TGGCACAAAA GGGACGAGGG GACCCCCTGG AGCAGCTGGC 
TACCCTGGGA ATCCCGGACT TCCTGGTATT CCTGGCCAAG ATGGCCCTCC AGGTCCCCCA 
GGTATCCCAG GATGCAACGG TACAAAGGGA GAGAGAGGGC CGCTCGGTCC TCCTGGCTTG 
CCTGGATTCA GCGGCAATCC TGGACCACCA GGGTTACCAG GAATGAAGGG AGATCCTGGT 
GAAATTCTCG GCCACGTTCC CGGAACACTG CTGAAAGGGG AGAGAGGATT TCCTGGTATC 
CCCGGGATGC CGGGCTCACC AGGGTTGCCA GGACTCCAGG GACCCGTGGG TCCTCCGGGA 
TTTACTGGAC CACCGGGTCC TCCAGGCCCT CCTGGACCTC CTGGAGAAAA GGGCCAGATG 
GGCTCCAGCT TCCAAGGACC AAAAGGTGAC AAGGGAGAGC AAGGGGTCAG CGGTCCCCCG 
GGAGTTCCTG GACAGGCACA AGTTAAGGAA AAAGGAGACT TTGCCCCAAC AGGAGAAAAG 
GGTCAGAAAG GTGAACCTGG ATTTCCGGGA GTACCAGGAT ATGGAGAGAA AGGTGAACCT 
GGCAAGCAAG GGCCTCGGGG AAAACCTGGG AAAGACGGTG AAAAAGGAGA AAGGGGGAGC 
CCGGGCATTC CTGGCGATTC CGGGTACCCA GGTCTCCCAG GCCGGCAGGG CCCGCAGGGA 
GAGAAGGGTG AAGCTGGACT TCCAGGCCCC CCTGGAACTG TGATAGGCAC CATGCCTTTG 
GGAGAGAAAG GAGATCGAGG CTACCCAGGA GCACCAGGGT TGAGAGGAGA GCCCGGCCCT 
AAAGGTTTCC CTGGAACACC AGGCCAACCA GGCCCTCCAG GTTTCCCTAC TCCAGGCCAG 
GCTGGTGCTC CAGGCTTCCC AGGTGAAAGG GGAGAAAAAG GTGACCAGGG ATTTCCAGGC 
GTGTCATTGC CAGGACCAAG TGGAAGAGAT GGAGCCCCGG GGCCTCCCGG CCCTCCCGGC 
CCCCCTGGGC AGCCAGGCCA CACAAATGGC ATTGTGGAGT GTCAACCTGG ACCACCCGGG 
GACCAGGGCC CTCCTGGGAC TCCAGGACAG CCAGGTTTGA CAGGTGAAGT TGGACAGAAA 
GGTCAGAAAG GAGAGAGCTG CCTTGCCTGT GACACAGAAG GACTTCGTGG CCCTCCAGGG 
CCACAGGGGC CTCCGGGAGA GATTGGTTTC CCTGGACAAC CTGGGGCTAA AGGTGACCGA 
GGCCTGCCCG GCAGAGATGG TCTTGAAGGA TTGCCGGGTC CACAAGGTTC ACCAGGGCTT 
ATAGGCCAGC CTGGAGCTAA GGGAGAGCCT GGAGAGATAT TTTTTGACAT GCGACTCAAA 
GGTGACAAAG GAGACCCAGG TTTTCCGGGA CAGCCTGGCA TGCCAGGAAG AGCAGGAACT 
CCCGGAAGAG ATGGCCACCC AGGACTCCCT GGACCCAAAG GCTCTCCGGG TTCAATAGGA 
TTGAAAGGAG AGCGAGGTCC TCCCGGAGGA GTTGGCTTCC CTGGTAGTCG TGGAGACATC 
GGCCCTCCTG GACCCCCAGG AGTTGGCCCC ATTGGTCCTG TTGGTGAAAA AGGACAAGCA 
GGCTTTCCTG GAGGCCCTGG GTCCCCAGGT CTTCCAGGCC CAAAGGGTGA GGCAGGAAAG 
GTTGTCCCAC TCCCTGGCCC CCCAGGAGCT GCAGGACTTC CAGGATCCCC TGGCTTCCCA 
GGGCCACAAG GTGACCGAGG CTTCCCAGGA ACCCCAGGAC GTCCAGGCAT CCCGGGAGAG 
AAAGGTGCTG TGGGCCAGCC AGGAATTGGA TTTCCTGGGC TTCCTGGCCC CAAAGGTGTT 
GATGGCTTGC CTGGAGAGAT AGGACGGCCT GGGAGTCCAG GTCGCCCTGG ATTTAACGGC 
TTACCTGGCA ACCCAGGACC TCAAGGTCAA AAGGGAGAAC CTGGCATTGG GCTTCCAGGA 
CTCAAAGGGC AACCAGGCCT TCCAGGCATT CCCGGTACAC CTGGAGAGAA GGGTAGCATC 
GGGGGACCCG GCGTTCCAGG AGAACAGGGG TTGACAGGCC CCCCAGGACT CCAGGGGATC 
AGAGGTGACC CAGGGCCTCC TGGAGTTCAA GGCCCAGCAG GTCCACCAGG GGTCCCAGGA 
ATAGGGCCAC CCGGAGCTAT GGGCCCTCCC GGAGGGCAAG GACCACCAGG GTCATCAGGT 
CCACCTGGAA TTAAAGGAGA GAAAGGGTTC CCTGGATTCC CCGGACTGGA TATGCCTGGC 
CCCAAAGGCG ATAAAGGCTC TCAAGGACTT CCTGGCCTCA CAGGACAGTC AGGCCTCCCT 
GGCCTTCCTG GACAGCAGGG GACACCTGGA GTTCCAGGGT TCCCAGGTTC TAAAGGTGAA 
ATGGGTGTCA TGGGAACCCC GGGACAACCA GGCTCGCCAG GACCAGCAGG CACCCCAGGG 
TTACCTGGAG AAAAAGGGGA CCATGGCCTT CCGGGCTCCT CAGGACCCAG GGGCGACCCT 
GGCTTCAAAG GTGATAAAGG TGACGTTGGG CTTCCTGGCA TGCCAGGATC CATGGAGCAT 
GTGGACATGG GGAGCATGAA GGGACAGAAA GGAGACCAGG GAGAGAAAGG ACAAATCGGA 
CCCACTGGTG ATAAAGGTTC CCGAGGAGAC CCTGGAACAC CAGGAGTACC TGGGAAGGAT 
GGGCAGGCAG GGCATCCCGG ACAGCCAGGG CCTAAAGGTG ACCCAGGCCT TAGTGGGACA 
CCAGGATCCC CTGGACTCCC TGGACCCAAA GGATCAGTTG GAGGAATGGG CTTGCCAGGT 
TCGCCTGGAG AAAAAGGTGT GCCTGGCATC CCTGGCTCAC AGGGTGTCCC TGGCTCACCT 
GGAGAGAAGG GAGCCAAAGG AGAGAAAGGG CAGTCAGGTC TACCTGGCAT TGGGATTCCG 
GGACGGCCTG GTGACAAGGG AGACCAGGGC CTTGCTGGCT TCCCAGGCAG CCCCGGTGAG 
AAGGGAGAGA AAGGCAGTGC CGGAACCCCA GGGATGCCAG GGTCCCCAGG CCCGAGAGGT 
TCTCCGGGGA ACATCGGCCA TCCAGGAAGC CCAGGCCTGC CTGGAGAGAA AGGGGATAAA 
GGCCTCCCAG GACTGGATGG CGTTCCTGGT GTCAAAGGAG AAGCAGGTCT CCCTGGGACT 
CCTGGCCCCA CAGGCCCAGC TGGCCAGAAG GGAGAGCCGG GCAGCGATGG AATCCCGGGG 
TCGGCAGGAG AGAAGGGTGA ACAAGGTGTT CCAGGAAGAG GCTTCCCCGG CTTCCCTGGC 
TCCAAAGGAG ACAAAGGCTC CAAGGGTGAA GTGGGTTTCC CTGGCCTAGC TGGAAGTCCT 
GGAATTCCTG GAGTCAAAGG CGAGCAAGGG TTCATGGGTC CTCCTGGCCC TCAAGGACAA 
CCTGGCTTAC CTGGCACTCC TGGTCACCCT GTGGAGGGGC CCAAAGGAGA CCGAGGACCT 
CAGGGTCAAC CTGGCCTGCC AGGGCATCCG GGACCTATGG GGCCGCCAGG GTTCCCAGGA 
ATCAATGGGC CAAAAGGTGA CAAGGGAAAT CAAGGTTGGC CAGGAGCTCC GGGGGTTCCA 
GGCCCTAAGG GAGACCCAGG ATTCCAAGGC ATGCCGGGCA TTGGCGGCTC TCCAGGGATC 
ACAGGTTCAA AGGGAGATAT GGGACTGCCC GGAGTTCCAG GATTTCAAGG TCAGAAAGGG 
CTTCCTGGTC TGCAGGGAGT GAAAGGAGAC CAGGGAGACC AAGGTGTACC CGGCCCTAAA 
GGTCTCCAAG GTCCCCCTGG GCCTCCAGGT CCCTACGATG TCATCAAAGG AGAACCAGGG 
CTCCCTGGTC CTGAGGGTCC CCCTGGTCTT AAAGGACTCC AGGGACCACC AGGTCCAAAA 
GGACAGCAAG GTGTGACAGG CTCAGTGGGC TTGCCTGGAC CTCCAGGTGT CCCTGGGTTC 
GATGGTGCTC CTGGCCAGAA AGGAGAGACA GGACCCTTTG GACCACCTGG TCCAAGAGGG 
TTTCCTGGCC CACCAGGCCC CGATGGGCTG CCAGGATCCA TGGGTCCCCC AGGTACCCCA 
TCTGTGGACC ATGGCTTCCT TGTGACCAGG CATAGTCAGA CAACAGATGA CCCACTGTGT 
CCCCCAGGGA CCAAAATTCT TTACCATGGA TACTCTCTGC TCTATGTCCA AGGCAACGAG 
CGTGCCCACG GGCAGGACTT GGGTACGGCT GGCAGCTGCC TGCGTAAGTT CAGCACCATG 
CCCTTTCTCT TCTGCAACAT CAACAACGTC TGCAACTTCG CCTCCAGGAA CGACTACTCT 
TACTGGCTGT CCACGCCAGA GCCCATGCCC ATGTCCATGG CACCCATCTC TGGGGACAAC 
ATCCGGCCCT TCATTAGCAG GTGTGCGGTT TGTGAAGCAC CGGCCATGGT GATGGCGGTA 
CACAGTCAGA CCATTCAGAT TCCGCAGTGC CCTAACGGTT GGTCCTCACT GTGGATCGGC 
TATTCCTTCG TGATGCACAC CAGCGCTGGT GCTGAAGGTT CCGGCCAAGC CCTCGCATCC 
CCCGGGTCCT GTCTGGAAGA GTTTAGAAGC GCCCCATTCA TCGAGTGCCA CGGCAGAGGA 
ACGTGCAATT ACTACGCAAA TGCTTACAGC TTTTGGCTCG CCACCATAGA GAGAAGCGAG 
ATGTTCAAGA AGCCCACGCC ATCCACCTTG AAGGCAGGGG AGCTGCGAAC ACACGTCAGC 
CGCTGCCAAG TGTGCATGAG AAGAACATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_034061.2
CDS	228..5237
Translation	MGPRLSVWLLLLFAALLLHEERSRAAAKGDCGGSGCGKCDCHGVKGQKGERGLPGLQGVIGFPGMQGPEGPHGPPGQKGDAGEPGLPGTKGTRGPPGAAGYPGNPGLPGIPGQDGPPGPPGIPGCNGTKGERGPLGPPGLPGFSGNPGPPGLPGMKGDPGEILGHVPGTLLKGERGFPGIPGMPGSPGLPGLQGPVGPPGFTGPPGPPGPPGPPGEKGQMGSSFQGPKGDKGEQGVSGPPGVPGQAQVKEKGDFAPTGEKGQKGEPGFPGVPGYGEKGEPGKQGPRGKPGKDGEKGERGSPGIPGDSGYPGLPGRQGPQGEKGEAGLPGPPGTVIGTMPLGEKGDRGYPGAPGLRGEPGPKGFPGTPGQPGPPGFPTPGQAGAPGFPGERGEKGDQGFPGVSLPGPSGRDGAPGPPGPPGPPGQPGHTNGIVECQPGPPGDQGPPGTPGQPGLTGEVGQKGQKGESCLACDTEGLRGPPGPQGPPGEIGFPGQPGAKGDRGLPGRDGLEGLPGPQGSPGLIGQPGAKGEPGEIFFDMRLKGDKGDPGFPGQPGMPGRAGTPGRDGHPGLPGPKGSPGSIGLKGERGPPGGVGFPGSRGDIGPPGPPGVGPIGPVGEKGQAGFPGGPGSPGLPGPKGEAGKVVPLPGPPGAAGLPGSPGFPGPQGDRGFPGTPGRPGIPGEKGAVGQPGIGFPGLPGPKGVDGLPGEIGRPGSPGRPGFNGLPGNPGPQGQKGEPGIGLPGLKGQPGLPGIPGTPGEKGSIGGPGVPGEQGLTGPPGLQGIRGDPGPPGVQGPAGPPGVPGIGPPGAMGPPGGQGPPGSSGPPGIKGEKGFPGFPGLDMPGPKGDKGSQGLPGLTGQSGLPGLPGQQGTPGVPGFPGSKGEMGVMGTPGQPGSPGPAGTPGLPGEKGDHGLPGSSGPRGDPGFKGDKGDVGLPGMPGSMEHVDMGSMKGQKGDQGEKGQIGPTGDKGSRGDPGTPGVPGKDGQAGHPGQPGPKGDPGLSGTPGSPGLPGPKGSVGGMGLPGSPGEKGVPGIPGSQGVPGSPGEKGAKGEKGQSGLPGIGIPGRPGDKGDQGLAGFPGSPGEKGEKGSAGTPGMPGSPGPRGSPGNIGHPGSPGLPGEKGDKGLPGLDGVPGVKGEAGLPGTPGPTGPAGQKGEPGSDGIPGSAGEKGEQGVPGRGFPGFPGSKGDKGSKGEVGFPGLAGSPGIPGVKGEQGFMGPPGPQGQPGLPGTPGHPVEGPKGDRGPQGQPGLPGHPGPMGPPGFPGINGPKGDKGNQGWPGAPGVPGPKGDPGFQGMPGIGGSPGITGSKGDMGLPGVPGFQGQKGLPGLQGVKGDQGDQGVPGPKGLQGPPGPPGPYDVIKGEPGLPGPEGPPGLKGLQGPPGPKGQQGVTGSVGLPGPPGVPGFDGAPGQKGETGPFGPPGPRGFPGPPGPDGLPGSMGPPGTPSVDHGFLVTRHSQTTDDPLCPPGTKILYHGYSLLYVQGNERAHGQDLGTAGSCLRKFSTMPFLFCNINNVCNFASRNDYSYWLSTPEPMPMSMAPISGDNIRPFISRCAVCEAPAMVMAVHSQTIQIPQCPNGWSSLWIGYSFVMHTSAGAEGSGQALASPGSCLEEFRSAPFIECHGRGTCNYYANAYSFWLATIERSEMFKKPTPSTLKAGELRTHVSRCQVCMRRT

Target ORF information:

RefSeq Version	NM_009931.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus collagen, type IV, alpha 1 (Col4a1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_009931.2

ORF Insert Sequence:

ATGGGGCCCC GGCTCAGCGT CTGGCTTCTG CTGCTCTTCG CCGCCCTTCT GCTCCACGAG 
GAGCGCAGCC GAGCAGCTGC GAAGGGCGAT TGTGGTGGCT CTGGCTGTGG AAAATGTGAC 
TGTCATGGCG TGAAGGGACA AAAGGGAGAA AGAGGCTTGC CAGGGTTGCA AGGTGTCATT 
GGATTTCCCG GGATGCAAGG ACCTGAGGGG CCACATGGAC CACCAGGACA AAAGGGTGAT 
GCTGGAGAAC CTGGACTTCC TGGCACAAAA GGGACGAGGG GACCCCCTGG AGCAGCTGGC 
TACCCTGGGA ATCCCGGACT TCCTGGTATT CCTGGCCAAG ATGGCCCTCC AGGTCCCCCA 
GGTATCCCAG GATGCAACGG TACAAAGGGA GAGAGAGGGC CGCTCGGTCC TCCTGGCTTG 
CCTGGATTCA GCGGCAATCC TGGACCACCA GGGTTACCAG GAATGAAGGG AGATCCTGGT 
GAAATTCTCG GCCACGTTCC CGGAACACTG CTGAAAGGGG AGAGAGGATT TCCTGGTATC 
CCCGGGATGC CGGGCTCACC AGGGTTGCCA GGACTCCAGG GACCCGTGGG TCCTCCGGGA 
TTTACTGGAC CACCGGGTCC TCCAGGCCCT CCTGGACCTC CTGGAGAAAA GGGCCAGATG 
GGCTCCAGCT TCCAAGGACC AAAAGGTGAC AAGGGAGAGC AAGGGGTCAG CGGTCCCCCG 
GGAGTTCCTG GACAGGCACA AGTTAAGGAA AAAGGAGACT TTGCCCCAAC AGGAGAAAAG 
GGTCAGAAAG GTGAACCTGG ATTTCCGGGA GTACCAGGAT ATGGAGAGAA AGGTGAACCT 
GGCAAGCAAG GGCCTCGGGG AAAACCTGGG AAAGACGGTG AAAAAGGAGA AAGGGGGAGC 
CCGGGCATTC CTGGCGATTC CGGGTACCCA GGTCTCCCAG GCCGGCAGGG CCCGCAGGGA 
GAGAAGGGTG AAGCTGGACT TCCAGGCCCC CCTGGAACTG TGATAGGCAC CATGCCTTTG 
GGAGAGAAAG GAGATCGAGG CTACCCAGGA GCACCAGGGT TGAGAGGAGA GCCCGGCCCT 
AAAGGTTTCC CTGGAACACC AGGCCAACCA GGCCCTCCAG GTTTCCCTAC TCCAGGCCAG 
GCTGGTGCTC CAGGCTTCCC AGGTGAAAGG GGAGAAAAAG GTGACCAGGG ATTTCCAGGC 
GTGTCATTGC CAGGACCAAG TGGAAGAGAT GGAGCCCCGG GGCCTCCCGG CCCTCCCGGC 
CCCCCTGGGC AGCCAGGCCA CACAAATGGC ATTGTGGAGT GTCAACCTGG ACCACCCGGG 
GACCAGGGCC CTCCTGGGAC TCCAGGACAG CCAGGTTTGA CAGGTGAAGT TGGACAGAAA 
GGTCAGAAAG GAGAGAGCTG CCTTGCCTGT GACACAGAAG GACTTCGTGG CCCTCCAGGG 
CCACAGGGGC CTCCGGGAGA GATTGGTTTC CCTGGACAAC CTGGGGCTAA AGGTGACCGA 
GGCCTGCCCG GCAGAGATGG TCTTGAAGGA TTGCCGGGTC CACAAGGTTC ACCAGGGCTT 
ATAGGCCAGC CTGGAGCTAA GGGAGAGCCT GGAGAGATAT TTTTTGACAT GCGACTCAAA 
GGTGACAAAG GAGACCCAGG TTTTCCGGGA CAGCCTGGCA TGCCAGGAAG AGCAGGAACT 
CCCGGAAGAG ATGGCCACCC AGGACTCCCT GGACCCAAAG GCTCTCCGGG TTCAATAGGA 
TTGAAAGGAG AGCGAGGTCC TCCCGGAGGA GTTGGCTTCC CTGGTAGTCG TGGAGACATC 
GGCCCTCCTG GACCCCCAGG AGTTGGCCCC ATTGGTCCTG TTGGTGAAAA AGGACAAGCA 
GGCTTTCCTG GAGGCCCTGG GTCCCCAGGT CTTCCAGGCC CAAAGGGTGA GGCAGGAAAG 
GTTGTCCCAC TCCCTGGCCC CCCAGGAGCT GCAGGACTTC CAGGATCCCC TGGCTTCCCA 
GGGCCACAAG GTGACCGAGG CTTCCCAGGA ACCCCAGGAC GTCCAGGCAT CCCGGGAGAG 
AAAGGTGCTG TGGGCCAGCC AGGAATTGGA TTTCCTGGGC TTCCTGGCCC CAAAGGTGTT 
GATGGCTTGC CTGGAGAGAT AGGACGGCCT GGGAGTCCAG GTCGCCCTGG ATTTAACGGC 
TTACCTGGCA ACCCAGGACC TCAAGGTCAA AAGGGAGAAC CTGGCATTGG GCTTCCAGGA 
CTCAAAGGGC AACCAGGCCT TCCAGGCATT CCCGGTACAC CTGGAGAGAA GGGTAGCATC 
GGGGGACCCG GCGTTCCAGG AGAACAGGGG TTGACAGGCC CCCCAGGACT CCAGGGGATC 
AGAGGTGACC CAGGGCCTCC TGGAGTTCAA GGCCCAGCAG GTCCACCAGG GGTCCCAGGA 
ATAGGGCCAC CCGGAGCTAT GGGCCCTCCC GGAGGGCAAG GACCACCAGG GTCATCAGGT 
CCACCTGGAA TTAAAGGAGA GAAAGGGTTC CCTGGATTCC CCGGACTGGA TATGCCTGGC 
CCCAAAGGCG ATAAAGGCTC TCAAGGACTT CCTGGCCTCA CAGGACAGTC AGGCCTCCCT 
GGCCTTCCTG GACAGCAGGG GACACCTGGA GTTCCAGGGT TCCCAGGTTC TAAAGGTGAA 
ATGGGTGTCA TGGGAACCCC GGGACAACCA GGCTCGCCAG GACCAGCAGG CACCCCAGGG 
TTACCTGGAG AAAAAGGGGA CCATGGCCTT CCGGGCTCCT CAGGACCCAG GGGCGACCCT 
GGCTTCAAAG GTGATAAAGG TGACGTTGGG CTTCCTGGCA TGCCAGGATC CATGGAGCAT 
GTGGACATGG GGAGCATGAA GGGACAGAAA GGAGACCAGG GAGAGAAAGG ACAAATCGGA 
CCCACTGGTG ATAAAGGTTC CCGAGGAGAC CCTGGAACAC CAGGAGTACC TGGGAAGGAT 
GGGCAGGCAG GGCATCCCGG ACAGCCAGGG CCTAAAGGTG ACCCAGGCCT TAGTGGGACA 
CCAGGATCCC CTGGACTCCC TGGACCCAAA GGATCAGTTG GAGGAATGGG CTTGCCAGGT 
TCGCCTGGAG AAAAAGGTGT GCCTGGCATC CCTGGCTCAC AGGGTGTCCC TGGCTCACCT 
GGAGAGAAGG GAGCCAAAGG AGAGAAAGGG CAGTCAGGTC TACCTGGCAT TGGGATTCCG 
GGACGGCCTG GTGACAAGGG AGACCAGGGC CTTGCTGGCT TCCCAGGCAG CCCCGGTGAG 
AAGGGAGAGA AAGGCAGTGC CGGAACCCCA GGGATGCCAG GGTCCCCAGG CCCGAGAGGT 
TCTCCGGGGA ACATCGGCCA TCCAGGAAGC CCAGGCCTGC CTGGAGAGAA AGGGGATAAA 
GGCCTCCCAG GACTGGATGG CGTTCCTGGT GTCAAAGGAG AAGCAGGTCT CCCTGGGACT 
CCTGGCCCCA CAGGCCCAGC TGGCCAGAAG GGAGAGCCGG GCAGCGATGG AATCCCGGGG 
TCGGCAGGAG AGAAGGGTGA ACAAGGTGTT CCAGGAAGAG GCTTCCCCGG CTTCCCTGGC 
TCCAAAGGAG ACAAAGGCTC CAAGGGTGAA GTGGGTTTCC CTGGCCTAGC TGGAAGTCCT 
GGAATTCCTG GAGTCAAAGG CGAGCAAGGG TTCATGGGTC CTCCTGGCCC TCAAGGACAA 
CCTGGCTTAC CTGGCACTCC TGGTCACCCT GTGGAGGGGC CCAAAGGAGA CCGAGGACCT 
CAGGGTCAAC CTGGCCTGCC AGGGCATCCG GGACCTATGG GGCCGCCAGG GTTCCCAGGA 
ATCAATGGGC CAAAAGGTGA CAAGGGAAAT CAAGGTTGGC CAGGAGCTCC GGGGGTTCCA 
GGCCCTAAGG GAGACCCAGG ATTCCAAGGC ATGCCGGGCA TTGGCGGCTC TCCAGGGATC 
ACAGGTTCAA AGGGAGATAT GGGACTGCCC GGAGTTCCAG GATTTCAAGG TCAGAAAGGG 
CTTCCTGGTC TGCAGGGAGT GAAAGGAGAC CAGGGAGACC AAGGTGTACC CGGCCCTAAA 
GGTCTCCAAG GTCCCCCTGG GCCTCCAGGT CCCTACGATG TCATCAAAGG AGAACCAGGG 
CTCCCTGGTC CTGAGGGTCC CCCTGGTCTT AAAGGACTCC AGGGACCACC AGGTCCAAAA 
GGACAGCAAG GTGTGACAGG CTCAGTGGGC TTGCCTGGAC CTCCAGGTGT CCCTGGGTTC 
GATGGTGCTC CTGGCCAGAA AGGAGAGACA GGACCCTTTG GACCACCTGG TCCAAGAGGG 
TTTCCTGGCC CACCAGGCCC CGATGGGCTG CCAGGATCCA TGGGTCCCCC AGGTACCCCA 
TCTGTGGACC ATGGCTTCCT TGTGACCAGG CATAGTCAGA CAACAGATGA CCCACTGTGT 
CCCCCAGGGA CCAAAATTCT TTACCATGGA TACTCTCTGC TCTATGTCCA AGGCAACGAG 
CGTGCCCACG GGCAGGACTT GGGTACGGCT GGCAGCTGCC TGCGTAAGTT CAGCACCATG 
CCCTTTCTCT TCTGCAACAT CAACAACGTC TGCAACTTCG CCTCCAGGAA CGACTACTCT 
TACTGGCTGT CCACGCCAGA GCCCATGCCC ATGTCCATGG CACCCATCTC TGGGGACAAC 
ATCCGGCCCT TCATTAGCAG GTGTGCGGTT TGTGAAGCAC CGGCCATGGT GATGGCGGTA 
CACAGTCAGA CCATTCAGAT TCCGCAGTGC CCTAACGGTT GGTCCTCACT GTGGATCGGC 
TATTCCTTCG TGATGCACAC CAGCGCTGGT GCTGAAGGTT CCGGCCAAGC CCTCGCATCC 
CCCGGGTCCT GTCTGGAAGA GTTTAGAAGC GCCCCATTCA TCGAGTGCCA CGGCAGAGGA 
ACGTGCAATT ACTACGCAAA TGCTTACAGC TTTTGGCTCG CCACCATAGA GAGAAGCGAG 
ATGTTCAAGA AGCCCACGCC ATCCACCTTG AAGGCAGGGG AGCTGCGAAC ACACGTCAGC 
CGCTGCCAAG TGTGCATGAG AAGAACATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Col4a1 ( XM_017312555.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017168044.1 Mus musculus collagen, type IV, alpha 1 (Col4a1), transcript variant X1, mRNA.

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CloneID	OMu82340
Clone ID Related Accession (Same CDS sequence)	XM_017312555.1 , XM_017312555.2
Accession Version	XM_017312555.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4818bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	collagen alpha-1(IV) chain isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000074.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGCCCC GGCTCAGCGT CTGGCTTCTG CTGCTCTTCG CCGCCCTTCT GCTCCACGAG 
GAGCGCAGCC GAGCAGCTGC GAAGGGCGAT TGTGGTGGCT CTGGCTGTGG AAAATGTGAC 
TGTCATGGCG TGAAGGGACA AAAGGGAGAA AGAGGCTTGC CAGGGTTGCA AGGTGTCATT 
GGATTTCCCG GGATGCAAGG ACCTGAGGGG CCACATGGAC CACCAGGACA AAAGGGTGAT 
GCTGGAGAAC CTGGACTTCC TGGCACAAAA GGGACGAGGG GACCCCCTGG AGCAGCTGGC 
TACCCTGGGA ATCCCGGACT TCCTGGTATT CCTGGCCAAG ATGGCCCTCC AGGTCCCCCA 
GGTATCCCAG GATGCAACGG TACAAAGGGA GAGAGAGGGC CGCTCGGTCC TCCTGGCTTG 
CCTGGATTCA GCGGCAATCC TGGACCACCA GGGTTACCAG GAATGAAGGG AGATCCTGGT 
GAAATTCTCG GCCACGTTCC CGGAACACTG CTGAAAGGGG AGAGAGGATT TCCTGGTATC 
CCCGGGATGC CGGGCTCACC AGGGTTGCCA GGACTCCAGG GACCCGTGGG TCCTCCGGGA 
TTTACTGGAC CACCGGGTCC TCCAGGCCCT CCTGGACCTC CTGGAGAAAA GGGCCAGATG 
GGCTCCAGCT TCCAAGGACC AAAAGGTGAC AAGGGAGAGC AAGGGGTCAG CGGTCCCCCG 
GGAGTTCCTG GACAGGCACA AGTTAAGGAA AAAGGAGACT TTGCCCCAAC AGGAGAAAAG 
GGTCAGAAAG GTGAACCTGG ATTTCCGGGA GTACCAGGAT ATGGAGAGAA AGGTGAACCT 
GGCAAGCAAG GGCCTCGGGG AAAACCTGGG AAAGACGGTG AAAAAGGAGA AAGGGGGAGC 
CCGGGCATTC CTGGCGATTC CGGGTACCCA GGTCTCCCAG GCCGGCAGGG CCCGCAGGGA 
GAGAAGGGTG AAGCTGGACT TCCAGGCCCC CCTGGAACTG TGATAGGCAC CATGCCTTTG 
GGAGAGAAAG GAGATCGAGG CTACCCAGGA GCACCAGGGT TGAGAGGAGA GCCCGGCCCT 
AAAGGTTTCC CTGGAACACC AGGCCAACCA GGCCCTCCAG GTTTCCCTAC TCCAGGCCAG 
GCTGGTGCTC CAGGCTTCCC AGGTGAAAGG GGAGAAAAAG GTGACCAGGG ATTTCCAGGC 
GTGTCATTGC CAGGACCAAG TGGAAGAGAT GGAGCCCCGG GGCCTCCCGG CCCTCCCGGC 
CCCCCTGGGC AGCCAGGCCA CACAAATGGC ATTGTGGAGT GTCAACCTGG ACCACCCGGG 
GACCAGGGCC CTCCTGGGAC TCCAGGACAG CCAGGTTTGA CAGGTGAAGT TGGACAGAAA 
GGTCAGAAAG GAGAGAGCTG CCTTGCCTGT GACACAGAAG GACTTCGTGG CCCTCCAGGG 
CCACAGGGGC CTCCGGGAGA GATTGGTTTC CCTGGACAAC CTGGGGCTAA AGGTGACCGA 
GGCCTGCCCG GCAGAGATGG TCTTGAAGGA TTGCCGGGTC CACAAGGTTC ACCAGGGCTT 
ATAGGCCAGC CTGGAGCTAA GGGAGAGCCT GGAGAGATAT TTTTTGACAT GCGACTCAAA 
GGTGACAAAG GAGACCCAGG TTTTCCGGGA CAGCCTGGCA TGCCAGGAAG AGCAGGAACT 
CCCGGAAGAG ATGGCCACCC AGGACTCCCT GGACCCAAAG GCTCTCCGGG TTCAATAGGA 
TTGAAAGGAG AGCGAGGTCC TCCCGGAGGA GTTGGCTTCC CTGGTAGTCG TGGAGACATC 
GGCCCTCCTG GACCCCCAGG AGTTGGCCCC ATTGGTCCTG TTGGTGAAAA AGGACAAGCA 
GGCTTTCCTG GAGGCCCTGG GTCCCCAGGT CTTCCAGGCC CAAAGGGTGA GGCAGGAAAG 
GTTGTCCCAC TCCCTGGCCC CCCAGGAGCT GCAGGACTTC CAGGATCCCC TGGCTTCCCA 
GGGCCACAAG GTGACCGAGG CTTCCCAGGA ACCCCAGGAC GTCCAGGCAT CCCGGGAGAG 
AAAGGTGCTG TGGGCCAGCC AGGAATTGGA TTTCCTGGGC TTCCTGGCCC CAAAGGTGTT 
GATGGCTTGC CTGGAGAGAT AGGACGGCCT GGGAGTCCAG GTCGCCCTGG ATTTAACGGC 
TTACCTGGCA ACCCAGGACC TCAAGGTCAA AAGGGAGAAC CTGGCATTGG GCTTCCAGGA 
CTCAAAGGGC AACCAGGCCT TCCAGGCATT CCCGGTACAC CTGGAGAGAA GGGTAGCATC 
GGGGGACCCG GCGTTCCAGG AGAACAGGGG TTGACAGGCC CCCCAGGACT CCAGGGGATC 
AGAGGTGACC CAGGGCCTCC TGGAGTTCAA GGCCCAGCAG GTCCACCAGG GGTCCCAGGA 
ATAGGGCCAC CCGGAGCTAT GGGCCCTCCC GGAGGGCAAG GACCACCAGG GTCATCAGGT 
CCACCTGGAA TTAAAGGAGA GAAAGGGTTC CCTGGATTCC CCGGACTGGA TATGCCTGGC 
CCCAAAGGCG ATAAAGGCTC TCAAGGACTT CCTGGCCTCA CAGGACAGTC AGGCCTCCCT 
GGCCTTCCTG GACAGCAGGG GACACCTGGA GTTCCAGGGT TCCCAGGTTC TAAAGGTGAA 
ATGGGTGTCA TGGGAACCCC GGGACAACCA GGCTCGCCAG GACCAGCAGG CACCCCAGGG 
TTACCTGGAG AAAAAGGGGA CCATGGCCTT CCGGGCTCCT CAGGACCCAG GGGCGACCCT 
GGCTTCAAAG GTGATAAAGG TGACGTTGGG CTTCCTGGCA TGCCAGGATC CATGGAGCAT 
GTGGACATGG GGAGCATGAA GGGACAGAAA GGAGACCAGG GAGAGAAAGG ACAAATCGGA 
CCCACTGGTG ATAAAGGTTC CCGAGGAGAC CCTGGAACAC CAGGAGTACC TGGGAAGGAT 
GGGCAGGCAG GGCATCCCGG ACAGCCAGGG CCTAAAGGTG ACCCAGGCCT TAGTGGGACA 
CCAGGATCCC CTGGACTCCC TGGACCCAAA GGATCAGTTG GAGGAATGGG CTTGCCAGGT 
TCGCCTGGAG AAAAAGGTGT GCCTGGCATC CCTGGCTCAC AGGGTGTCCC TGGCTCACCT 
GGAGAGAAGG GAGCCAAAGG AGAGAAAGGG CAGTCAGGTC TACCTGGCAT TGGGATTCCG 
GGACGGCCTG GTGACAAGGG AGACCAGGGC CTTGCTGGCT TCCCAGGCAG CCCCGGTGAG 
AAGGGAGAGA AAGGCAGTGC CGGAACCCCA GGGATGCCAG GGTCCCCAGG CCCGAGAGGT 
TCTCCGGGGA ACATCGGCCA TCCAGGAAGC CCAGGCCTGC CTGGAGAGAA AGGGGATAAA 
GGCCTCCCAG GACTGGATGG CGTTCCTGGT GTCAAAGGAG AAGCAGGTCT CCCTGGGACT 
CCTGGCCCCA CAGGCCCAGC TGGCCAGAAG GGAGAGCCGG GCAGCGATGG AATCCCGGGG 
TCGGCAGGAG AGAAGGGTGA ACAAGGTGTT CCAGGAAGAG GCTTCCCCGG CTTCCCTGGC 
TCCAAAGGAG ACAAAGGCTC CAAGGGTGAA GTGGGTTTCC CTGGCCTAGC TGGAAGTCCT 
GGAATTCCTG GAGTCAAAGG CGAGCAAGGG TTCATGGGTC CTCCTGGCCC TCAAGGACAA 
CCTGGCTTAC CTGGCACTCC TGGTCACCCT GTGGAGGGGC CCAAAGGAGA CCGAGGACCT 
CAGGGTCAAC CTGGCCTGCC AGGGCATCCG GGACCTATGG GGCCGCCAGG GTTCCCAGGA 
ATCAATGGGC CAAAAGGTGA CAAGGGAAAT CAAGGTTGGC CAGGAGCTCC GGGGGTTCCA 
GGCCCTAAGG GAGACCCAGG ATTCCAAGGC ATGCCGGGCA TTGGCGGCTC TCCAGGGATC 
ACAGGTTCAA AGGGAGATAT GGGACTGCCC GGAGTTCCAG GATTTCAAGG TCAGAAAGGG 
CTTCCTGGTC TGCAGGGAGT GAAAGGAGAC CAGGGAGACC AAGGTGTACC CGGCCCTAAA 
GGTCTCCAAG GTCCCCCTGG GCCTCCAGGT CCCTACGATG TCATCAAAGG AGAACCAGGG 
CTCCCTGGTC CTGAGGGTCC CCCTGGTCTT AAAGGACTCC AGGGACCACC AGGTCCAAAA 
GGACAGCAAG GTGTGACAGG CTCAGTGGGC TTGCCTGGAC CTCCAGGTGT CCCTGGGTTC 
GATGGTGCTC CTGGCCAGAA AGGAGAGACA GGACCCTTTG GACCACCTGG TCCAAGAGGG 
TTTCCTGGCC CACCAGGCCC CGATGGGCTG CCAGGATCCA TGGGTCCCCC AGGTACCCCA 
TCTGTGGACC ATGGCTTCCT TGTGACCAGG CATAGTCAGA CAACAGATGA CCCACTGTGT 
CCCCCAGGGA CCAAAATTCT TTACCATGGA TACTCTCTGC TCTATGTCCA AGGCAACGAG 
CGTGCCCACG GGCAGGACTT GGGTACGGCT GGCAGCTGCC TGCGTAAGTT CAGCACCATG 
CCCTTTCTCT TCTGCAACAT CAACAACGTC TGCAACTTCG CCTCCAGGAA CGACTACTCT 
TACTGGCTGT CCACGCCAGA GCCCATGCCC ATGTCCATGG CACCCATCTC TGGGGACAAC 
ATCCGGCCCT TCATTAGCAG CTTTCCTTGG AAAACAGGTA GAAAATGTTT ATTATTGATG 
TCTCTTGGTT ACTTTTGGAA TGGCGTCTAT GCTGGTCTTG ACTTGGTTGA AGACACAGGC 
CCAGGGAAAC TGTGTGTCTC ATTCTTTTCT CCATGGGCAT TTGTCTTCAC AAAGTTCCTG 
TTCTGTGAGC ACACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017168044.1
CDS	155..4972
Translation	MGPRLSVWLLLLFAALLLHEERSRAAAKGDCGGSGCGKCDCHGVKGQKGERGLPGLQGVIGFPGMQGPEGPHGPPGQKGDAGEPGLPGTKGTRGPPGAAGYPGNPGLPGIPGQDGPPGPPGIPGCNGTKGERGPLGPPGLPGFSGNPGPPGLPGMKGDPGEILGHVPGTLLKGERGFPGIPGMPGSPGLPGLQGPVGPPGFTGPPGPPGPPGPPGEKGQMGSSFQGPKGDKGEQGVSGPPGVPGQAQVKEKGDFAPTGEKGQKGEPGFPGVPGYGEKGEPGKQGPRGKPGKDGEKGERGSPGIPGDSGYPGLPGRQGPQGEKGEAGLPGPPGTVIGTMPLGEKGDRGYPGAPGLRGEPGPKGFPGTPGQPGPPGFPTPGQAGAPGFPGERGEKGDQGFPGVSLPGPSGRDGAPGPPGPPGPPGQPGHTNGIVECQPGPPGDQGPPGTPGQPGLTGEVGQKGQKGESCLACDTEGLRGPPGPQGPPGEIGFPGQPGAKGDRGLPGRDGLEGLPGPQGSPGLIGQPGAKGEPGEIFFDMRLKGDKGDPGFPGQPGMPGRAGTPGRDGHPGLPGPKGSPGSIGLKGERGPPGGVGFPGSRGDIGPPGPPGVGPIGPVGEKGQAGFPGGPGSPGLPGPKGEAGKVVPLPGPPGAAGLPGSPGFPGPQGDRGFPGTPGRPGIPGEKGAVGQPGIGFPGLPGPKGVDGLPGEIGRPGSPGRPGFNGLPGNPGPQGQKGEPGIGLPGLKGQPGLPGIPGTPGEKGSIGGPGVPGEQGLTGPPGLQGIRGDPGPPGVQGPAGPPGVPGIGPPGAMGPPGGQGPPGSSGPPGIKGEKGFPGFPGLDMPGPKGDKGSQGLPGLTGQSGLPGLPGQQGTPGVPGFPGSKGEMGVMGTPGQPGSPGPAGTPGLPGEKGDHGLPGSSGPRGDPGFKGDKGDVGLPGMPGSMEHVDMGSMKGQKGDQGEKGQIGPTGDKGSRGDPGTPGVPGKDGQAGHPGQPGPKGDPGLSGTPGSPGLPGPKGSVGGMGLPGSPGEKGVPGIPGSQGVPGSPGEKGAKGEKGQSGLPGIGIPGRPGDKGDQGLAGFPGSPGEKGEKGSAGTPGMPGSPGPRGSPGNIGHPGSPGLPGEKGDKGLPGLDGVPGVKGEAGLPGTPGPTGPAGQKGEPGSDGIPGSAGEKGEQGVPGRGFPGFPGSKGDKGSKGEVGFPGLAGSPGIPGVKGEQGFMGPPGPQGQPGLPGTPGHPVEGPKGDRGPQGQPGLPGHPGPMGPPGFPGINGPKGDKGNQGWPGAPGVPGPKGDPGFQGMPGIGGSPGITGSKGDMGLPGVPGFQGQKGLPGLQGVKGDQGDQGVPGPKGLQGPPGPPGPYDVIKGEPGLPGPEGPPGLKGLQGPPGPKGQQGVTGSVGLPGPPGVPGFDGAPGQKGETGPFGPPGPRGFPGPPGPDGLPGSMGPPGTPSVDHGFLVTRHSQTTDDPLCPPGTKILYHGYSLLYVQGNERAHGQDLGTAGSCLRKFSTMPFLFCNINNVCNFASRNDYSYWLSTPEPMPMSMAPISGDNIRPFISSFPWKTGRKCLLLMSLGYFWNGVYAGLDLVEDTGPGKLCVSFFSPWAFVFTKFLFCEHT

Target ORF information:

RefSeq Version	XM_017312555.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus collagen, type IV, alpha 1 (Col4a1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017312555.1

ORF Insert Sequence:

ATGGGGCCCC GGCTCAGCGT CTGGCTTCTG CTGCTCTTCG CCGCCCTTCT GCTCCACGAG 
GAGCGCAGCC GAGCAGCTGC GAAGGGCGAT TGTGGTGGCT CTGGCTGTGG AAAATGTGAC 
TGTCATGGCG TGAAGGGACA AAAGGGAGAA AGAGGCTTGC CAGGGTTGCA AGGTGTCATT 
GGATTTCCCG GGATGCAAGG ACCTGAGGGG CCACATGGAC CACCAGGACA AAAGGGTGAT 
GCTGGAGAAC CTGGACTTCC TGGCACAAAA GGGACGAGGG GACCCCCTGG AGCAGCTGGC 
TACCCTGGGA ATCCCGGACT TCCTGGTATT CCTGGCCAAG ATGGCCCTCC AGGTCCCCCA 
GGTATCCCAG GATGCAACGG TACAAAGGGA GAGAGAGGGC CGCTCGGTCC TCCTGGCTTG 
CCTGGATTCA GCGGCAATCC TGGACCACCA GGGTTACCAG GAATGAAGGG AGATCCTGGT 
GAAATTCTCG GCCACGTTCC CGGAACACTG CTGAAAGGGG AGAGAGGATT TCCTGGTATC 
CCCGGGATGC CGGGCTCACC AGGGTTGCCA GGACTCCAGG GACCCGTGGG TCCTCCGGGA 
TTTACTGGAC CACCGGGTCC TCCAGGCCCT CCTGGACCTC CTGGAGAAAA GGGCCAGATG 
GGCTCCAGCT TCCAAGGACC AAAAGGTGAC AAGGGAGAGC AAGGGGTCAG CGGTCCCCCG 
GGAGTTCCTG GACAGGCACA AGTTAAGGAA AAAGGAGACT TTGCCCCAAC AGGAGAAAAG 
GGTCAGAAAG GTGAACCTGG ATTTCCGGGA GTACCAGGAT ATGGAGAGAA AGGTGAACCT 
GGCAAGCAAG GGCCTCGGGG AAAACCTGGG AAAGACGGTG AAAAAGGAGA AAGGGGGAGC 
CCGGGCATTC CTGGCGATTC CGGGTACCCA GGTCTCCCAG GCCGGCAGGG CCCGCAGGGA 
GAGAAGGGTG AAGCTGGACT TCCAGGCCCC CCTGGAACTG TGATAGGCAC CATGCCTTTG 
GGAGAGAAAG GAGATCGAGG CTACCCAGGA GCACCAGGGT TGAGAGGAGA GCCCGGCCCT 
AAAGGTTTCC CTGGAACACC AGGCCAACCA GGCCCTCCAG GTTTCCCTAC TCCAGGCCAG 
GCTGGTGCTC CAGGCTTCCC AGGTGAAAGG GGAGAAAAAG GTGACCAGGG ATTTCCAGGC 
GTGTCATTGC CAGGACCAAG TGGAAGAGAT GGAGCCCCGG GGCCTCCCGG CCCTCCCGGC 
CCCCCTGGGC AGCCAGGCCA CACAAATGGC ATTGTGGAGT GTCAACCTGG ACCACCCGGG 
GACCAGGGCC CTCCTGGGAC TCCAGGACAG CCAGGTTTGA CAGGTGAAGT TGGACAGAAA 
GGTCAGAAAG GAGAGAGCTG CCTTGCCTGT GACACAGAAG GACTTCGTGG CCCTCCAGGG 
CCACAGGGGC CTCCGGGAGA GATTGGTTTC CCTGGACAAC CTGGGGCTAA AGGTGACCGA 
GGCCTGCCCG GCAGAGATGG TCTTGAAGGA TTGCCGGGTC CACAAGGTTC ACCAGGGCTT 
ATAGGCCAGC CTGGAGCTAA GGGAGAGCCT GGAGAGATAT TTTTTGACAT GCGACTCAAA 
GGTGACAAAG GAGACCCAGG TTTTCCGGGA CAGCCTGGCA TGCCAGGAAG AGCAGGAACT 
CCCGGAAGAG ATGGCCACCC AGGACTCCCT GGACCCAAAG GCTCTCCGGG TTCAATAGGA 
TTGAAAGGAG AGCGAGGTCC TCCCGGAGGA GTTGGCTTCC CTGGTAGTCG TGGAGACATC 
GGCCCTCCTG GACCCCCAGG AGTTGGCCCC ATTGGTCCTG TTGGTGAAAA AGGACAAGCA 
GGCTTTCCTG GAGGCCCTGG GTCCCCAGGT CTTCCAGGCC CAAAGGGTGA GGCAGGAAAG 
GTTGTCCCAC TCCCTGGCCC CCCAGGAGCT GCAGGACTTC CAGGATCCCC TGGCTTCCCA 
GGGCCACAAG GTGACCGAGG CTTCCCAGGA ACCCCAGGAC GTCCAGGCAT CCCGGGAGAG 
AAAGGTGCTG TGGGCCAGCC AGGAATTGGA TTTCCTGGGC TTCCTGGCCC CAAAGGTGTT 
GATGGCTTGC CTGGAGAGAT AGGACGGCCT GGGAGTCCAG GTCGCCCTGG ATTTAACGGC 
TTACCTGGCA ACCCAGGACC TCAAGGTCAA AAGGGAGAAC CTGGCATTGG GCTTCCAGGA 
CTCAAAGGGC AACCAGGCCT TCCAGGCATT CCCGGTACAC CTGGAGAGAA GGGTAGCATC 
GGGGGACCCG GCGTTCCAGG AGAACAGGGG TTGACAGGCC CCCCAGGACT CCAGGGGATC 
AGAGGTGACC CAGGGCCTCC TGGAGTTCAA GGCCCAGCAG GTCCACCAGG GGTCCCAGGA 
ATAGGGCCAC CCGGAGCTAT GGGCCCTCCC GGAGGGCAAG GACCACCAGG GTCATCAGGT 
CCACCTGGAA TTAAAGGAGA GAAAGGGTTC CCTGGATTCC CCGGACTGGA TATGCCTGGC 
CCCAAAGGCG ATAAAGGCTC TCAAGGACTT CCTGGCCTCA CAGGACAGTC AGGCCTCCCT 
GGCCTTCCTG GACAGCAGGG GACACCTGGA GTTCCAGGGT TCCCAGGTTC TAAAGGTGAA 
ATGGGTGTCA TGGGAACCCC GGGACAACCA GGCTCGCCAG GACCAGCAGG CACCCCAGGG 
TTACCTGGAG AAAAAGGGGA CCATGGCCTT CCGGGCTCCT CAGGACCCAG GGGCGACCCT 
GGCTTCAAAG GTGATAAAGG TGACGTTGGG CTTCCTGGCA TGCCAGGATC CATGGAGCAT 
GTGGACATGG GGAGCATGAA GGGACAGAAA GGAGACCAGG GAGAGAAAGG ACAAATCGGA 
CCCACTGGTG ATAAAGGTTC CCGAGGAGAC CCTGGAACAC CAGGAGTACC TGGGAAGGAT 
GGGCAGGCAG GGCATCCCGG ACAGCCAGGG CCTAAAGGTG ACCCAGGCCT TAGTGGGACA 
CCAGGATCCC CTGGACTCCC TGGACCCAAA GGATCAGTTG GAGGAATGGG CTTGCCAGGT 
TCGCCTGGAG AAAAAGGTGT GCCTGGCATC CCTGGCTCAC AGGGTGTCCC TGGCTCACCT 
GGAGAGAAGG GAGCCAAAGG AGAGAAAGGG CAGTCAGGTC TACCTGGCAT TGGGATTCCG 
GGACGGCCTG GTGACAAGGG AGACCAGGGC CTTGCTGGCT TCCCAGGCAG CCCCGGTGAG 
AAGGGAGAGA AAGGCAGTGC CGGAACCCCA GGGATGCCAG GGTCCCCAGG CCCGAGAGGT 
TCTCCGGGGA ACATCGGCCA TCCAGGAAGC CCAGGCCTGC CTGGAGAGAA AGGGGATAAA 
GGCCTCCCAG GACTGGATGG CGTTCCTGGT GTCAAAGGAG AAGCAGGTCT CCCTGGGACT 
CCTGGCCCCA CAGGCCCAGC TGGCCAGAAG GGAGAGCCGG GCAGCGATGG AATCCCGGGG 
TCGGCAGGAG AGAAGGGTGA ACAAGGTGTT CCAGGAAGAG GCTTCCCCGG CTTCCCTGGC 
TCCAAAGGAG ACAAAGGCTC CAAGGGTGAA GTGGGTTTCC CTGGCCTAGC TGGAAGTCCT 
GGAATTCCTG GAGTCAAAGG CGAGCAAGGG TTCATGGGTC CTCCTGGCCC TCAAGGACAA 
CCTGGCTTAC CTGGCACTCC TGGTCACCCT GTGGAGGGGC CCAAAGGAGA CCGAGGACCT 
CAGGGTCAAC CTGGCCTGCC AGGGCATCCG GGACCTATGG GGCCGCCAGG GTTCCCAGGA 
ATCAATGGGC CAAAAGGTGA CAAGGGAAAT CAAGGTTGGC CAGGAGCTCC GGGGGTTCCA 
GGCCCTAAGG GAGACCCAGG ATTCCAAGGC ATGCCGGGCA TTGGCGGCTC TCCAGGGATC 
ACAGGTTCAA AGGGAGATAT GGGACTGCCC GGAGTTCCAG GATTTCAAGG TCAGAAAGGG 
CTTCCTGGTC TGCAGGGAGT GAAAGGAGAC CAGGGAGACC AAGGTGTACC CGGCCCTAAA 
GGTCTCCAAG GTCCCCCTGG GCCTCCAGGT CCCTACGATG TCATCAAAGG AGAACCAGGG 
CTCCCTGGTC CTGAGGGTCC CCCTGGTCTT AAAGGACTCC AGGGACCACC AGGTCCAAAA 
GGACAGCAAG GTGTGACAGG CTCAGTGGGC TTGCCTGGAC CTCCAGGTGT CCCTGGGTTC 
GATGGTGCTC CTGGCCAGAA AGGAGAGACA GGACCCTTTG GACCACCTGG TCCAAGAGGG 
TTTCCTGGCC CACCAGGCCC CGATGGGCTG CCAGGATCCA TGGGTCCCCC AGGTACCCCA 
TCTGTGGACC ATGGCTTCCT TGTGACCAGG CATAGTCAGA CAACAGATGA CCCACTGTGT 
CCCCCAGGGA CCAAAATTCT TTACCATGGA TACTCTCTGC TCTATGTCCA AGGCAACGAG 
CGTGCCCACG GGCAGGACTT GGGTACGGCT GGCAGCTGCC TGCGTAAGTT CAGCACCATG 
CCCTTTCTCT TCTGCAACAT CAACAACGTC TGCAACTTCG CCTCCAGGAA CGACTACTCT 
TACTGGCTGT CCACGCCAGA GCCCATGCCC ATGTCCATGG CACCCATCTC TGGGGACAAC 
ATCCGGCCCT TCATTAGCAG CTTTCCTTGG AAAACAGGTA GAAAATGTTT ATTATTGATG 
TCTCTTGGTT ACTTTTGGAA TGGCGTCTAT GCTGGTCTTG ACTTGGTTGA AGACACAGGC 
CCAGGGAAAC TGTGTGTCTC ATTCTTTTCT CCATGGGCAT TTGTCTTCAC AAAGTTCCTG 
TTCTGTGAGC ACACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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Col4a1 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Most Popular Services

Services & Products

見積依頼

Eメール

電話

FAX

オンラインフォーム

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サポート

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