Cmas cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Cmas
Entrez Gene ID	12764
Full Name	cytidine monophospho-N-acetylneuraminic acid synthetase
Synonyms	AW208911,CMPNeu5Ac,D6Bwg0250e
General protein information	Preferred Names cytidine monophospho-N-acetylneuraminic acid synthetase Names N-acylneuraminate cytidylyltransferase CMP-N-acetylneuraminic acid synthase CMP-N-acetylneuraminic acid synthetase CMP-Neu5Ac synthase CMP-NeuNAc synthase CMP-NeuNAc synthetase CMP-sialic acid synthetase
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 6 Map Location: 6 G2\|6 74.66 cM

mRNA and Protein(s)

mRNA	Protein	Name
XM_030255134.1	XP_030110994.1	N-acylneuraminate cytidylyltransferase isoform X1
NM_009908.2	NP_034038.2	N-acylneuraminate cytidylyltransferase

Pathways from BioSystems

Homologies

	CMAS	XP_001099303.1
Mus musculus (house mouse)	Cmas	NP_034038.2
Danio rerio (zebrafish)	cmasb	NP_001245338.1
Rattus norvegicus (Norway rat)	Cmas	NP_001009419.1
Danio rerio (zebrafish)	cmasa	NP_001035342.2
Homo sapiens (human)	CMAS	NP_061156.1
Gallus gallus (chicken)	CMAS	XP_416429.1
Drosophila melanogaster (fruit fly)	Csas	NP_730474.1
Pan troglodytes (chimpanzee)	CMAS	XP_520794.2
Canis lupus familiaris (dog)	CMAS	XP_534866.1
Bos taurus (cattle)	CMAS	NP_001030475.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP004527	XP_313827.5

Gene Ontology
Bibliography

Related articles in PubMed

Sialylation Is Dispensable for Early Murine Embryonic Development in Vitro.
Abeln M, Borst KM, Cajic S, Thiesler H, Kats E, Albers I, Kuhn M, Kaever V, Rapp E, M?nster-K?hnel A, Weinhold B
Chembiochem : a European journal of chemical biology18(13)1305-1316(2017 07)

A C-terminal phosphatase module conserved in vertebrate CMP-sialic acid synthetases provides a tetramerization interface for the physiologically active enzyme.
Oschlies M, Dickmanns A, Haselhorst T, Schaper W, Stummeyer K, Tiralongo J, Weinhold B, Gerardy-Schahn R, von Itzstein M, Ficner R, M?nster-K?hnel AK
Journal of molecular biology393(1)83-97(2009 Oct)

The rainbow trout CMP-sialic acid synthetase utilises a nuclear localization signal different from that identified in the mouse enzyme.
Tiralongo J, Fujita A, Sato C, Kitajima K, Lehmann F, Oschlies M, Gerardy-Schahn R, M?nster-K?hnel AK
Glycobiology17(9)945-54(2007 Sep)

Identification of the nuclear export signals that regulate the intracellular localization of the mouse CMP-sialic acid synthetase.
Fujita A, Sato C, Kitajima K
Biochemical and biophysical research communications355(1)174-80(2007 Mar)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Sialylation is dispensable for early murine embryonic development in vitro in CMAS-null embryonic stem cells.
Title: Sialylation Is Dispensable for Early Murine Embryonic Development in Vitro.

The authors provide evidence that the C-terminal domain of CMP-Sialic Acid Synthetase (CSS) serves as a platform for the quaternary organization governing the kinetic properties of the physiologically active enzyme as demonstrated in kinetic studies.[CSS]
Title: A C-terminal phosphatase module conserved in vertebrate CMP-sialic acid synthetases provides a tetramerization interface for the physiologically active enzyme.

the rainbow trout CMP-sialic acid synthetase utilises a nuclear localization signal different from that identified in the mouse enzyme
Title: The rainbow trout CMP-sialic acid synthetase utilises a nuclear localization signal different from that identified in the mouse enzyme.

results describe the crystal structure of the N-terminal catalytically active domain of the murine 5-N-acetylneuraminic acid synthetase in complex with the reaction product
Title: The crystal structure of murine CMP-5-N-acetylneuraminic acid synthetase.

Nuclear localization signal of murine CMP-Neu5Ac synthetase includes residues required for both nuclear targeting and enzymatic activity
Title: Nuclear localization signal of murine CMP-Neu5Ac synthetase includes residues required for both nuclear targeting and enzymatic activity.

The following Cmas gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Cmas cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu89118	XM_030255134.1 Latest version!	Mus musculus cytidine monophospho-N-acetylneuraminic acid synthetase (Cmas), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$139.30 $199.00
OMu05120	NM_009908.2 Latest version!	Mus musculus cytidine monophospho-N-acetylneuraminic acid synthetase (Cmas), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$188.30 $269.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Cmas ( XM_030255134.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030110994.1 Mus musculus cytidine monophospho-N-acetylneuraminic acid synthetase (Cmas), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu89118
Clone ID Related Accession (Same CDS sequence)	XM_030255134.1
Accession Version	XM_030255134.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 828bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	N-acylneuraminate cytidylyltransferase isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000072.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATCCGAG AAGAAGGATA TGACTCTGTC TTCTCCGTTG TGAGGCGCCA TCAGTTTCGA 
TGGAGTGAAA TTCAGAAAGG AGTTCGTGAA GTGACTGAGC CTCTGAACTT GAATCCAGCG 
AAACGGCCTC GTCGACAAGA CTGGGATGGA GAGTTATATG AGAACGGCTC ATTTTATTTT 
GCTAAAAGAC ATTTGATAGA GATGGGTTAC TTACAGGGTG GGAAAATGGC ATATTATGAA 
ATGCGAGCTG AGCACAGTGT GGATATCGAC GTGGACATCG ATTGGCCGAT CGCAGAGCAA 
AGAGTTCTGA GATTTGGCTA TTTTGGAAAA GAGAAGCTGA AGGAGATAAA GCTTTTGGTT 
TGTAATATTG ATGGATGTCT CACCAATGGC CACATTTATG TATCAGGAGA CCAAAAAGAA 
ATAATATCTT ATGATGTAAA AGACGCTATT GGCATAAGTT TATTAAAGAA AAGCGGTATT 
GAGGTGAGGC TCATCTCAGA ACGGGCCTGC TCCAAGCAGA CGCTCTCTGC CCTAAAGCTG 
GACTGTAAAA CAGAAGTCAG TGTGTCCGAT AAGCTGGCCA CCGTGGATGA GTGGAGGAAG 
GAGATGGGCC TGTGCTGGAA AGAAGTGGCC TATCTCGGCA ATGAAGTGTC TGATGAAGAA 
TGCCTCAAGA GAGTGGGCCT GAGCGCTGTT CCTGCCGACG CCTGCTCCGG GGCCCAGAAG 
GCTGTGGGGT ACATCTGCAA ATGCAGCGGT GGCCGGGGAG CCATCCGCGA GTTTGCAGAG 
CACATTTTCC TACTGATAGA AAAAGTTAAT AACTCATGCC AAAAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030110994.1
CDS	476..1303
Translation	MIREEGYDSVFSVVRRHQFRWSEIQKGVREVTEPLNLNPAKRPRRQDWDGELYENGSFYFAKRHLIEMGYLQGGKMAYYEMRAEHSVDIDVDIDWPIAEQRVLRFGYFGKEKLKEIKLLVCNIDGCLTNGHIYVSGDQKEIISYDVKDAIGISLLKKSGIEVRLISERACSKQTLSALKLDCKTEVSVSDKLATVDEWRKEMGLCWKEVAYLGNEVSDEECLKRVGLSAVPADACSGAQKAVGYICKCSGGRGAIREFAEHIFLLIEKVNNSCQK

Target ORF information:

RefSeq Version	XM_030255134.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus cytidine monophospho-N-acetylneuraminic acid synthetase (Cmas), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030255134.1

ORF Insert Sequence:

ATGATCCGAG AAGAAGGATA TGACTCTGTC TTCTCCGTTG TGAGGCGCCA TCAGTTTCGA 
TGGAGTGAAA TTCAGAAAGG AGTTCGTGAA GTGACTGAGC CTCTGAACTT GAATCCAGCG 
AAACGGCCTC GTCGACAAGA CTGGGATGGA GAGTTATATG AGAACGGCTC ATTTTATTTT 
GCTAAAAGAC ATTTGATAGA GATGGGTTAC TTACAGGGTG GGAAAATGGC ATATTATGAA 
ATGCGAGCTG AGCACAGTGT GGATATCGAC GTGGACATCG ATTGGCCGAT CGCAGAGCAA 
AGAGTTCTGA GATTTGGCTA TTTTGGAAAA GAGAAGCTGA AGGAGATAAA GCTTTTGGTT 
TGTAATATTG ATGGATGTCT CACCAATGGC CACATTTATG TATCAGGAGA CCAAAAAGAA 
ATAATATCTT ATGATGTAAA AGACGCTATT GGCATAAGTT TATTAAAGAA AAGCGGTATT 
GAGGTGAGGC TCATCTCAGA ACGGGCCTGC TCCAAGCAGA CGCTCTCTGC CCTAAAGCTG 
GACTGTAAAA CAGAAGTCAG TGTGTCCGAT AAGCTGGCCA CCGTGGATGA GTGGAGGAAG 
GAGATGGGCC TGTGCTGGAA AGAAGTGGCC TATCTCGGCA ATGAAGTGTC TGATGAAGAA 
TGCCTCAAGA GAGTGGGCCT GAGCGCTGTT CCTGCCGACG CCTGCTCCGG GGCCCAGAAG 
GCTGTGGGGT ACATCTGCAA ATGCAGCGGT GGCCGGGGAG CCATCCGCGA GTTTGCAGAG 
CACATTTTCC TACTGATAGA AAAAGTTAAT AACTCATGCC AAAAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cmas ( NM_009908.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_034038.2 Mus musculus cytidine monophospho-N-acetylneuraminic acid synthetase (Cmas), mRNA.

Price & Availability↑

CloneID	OMu05120
Clone ID Related Accession (Same CDS sequence)	NM_009908.2
Accession Version	NM_009908.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1299bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-24
Organism	Mus musculus(house mouse)
Product	N-acylneuraminate cytidylyltransferase
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from CJ161865.1, AK155730.1 and BQ033546.1. On Jul 2, 2009 this sequence version replaced NM_009908.1. ##Evidence-Data-START## Transcript exon combination :: AK155730.1, BC063776.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849377 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGACGCGC TGGAGAAGGG GGCCGCCACG TCGGGGCCCG CCCCGCGTGG ACGGCCGTCC 
CGGGGCCGGC CCCCGAAGCT GCAGCGCAGC CGGGGCGCGG GGCGCGGCCT AGAGAAGCCG 
CCGCACCTGG CAGCGCTGGT GCTGGCCCGC GGCGGCAGCA AAGGCATCCC ACTGAAGAAC 
ATCAAGCGCC TGGCGGGGGT TCCGCTCATT GGCTGGGTCC TGCGCGCCGC CCTGGATGCG 
GGGGTCTTCC AGAGTGTGTG GGTTTCAACA GACCATGATG AAATTGAGAA TGTGGCCAAA 
CAGTTTGGTG CACAGGTCCA TCGAAGAAGT TCTGAAACGT CCAAAGACAG CTCTACCTCA 
CTAGACGCCA TTGTAGAATT CCTGAATTAT CACAATGAGG TTGACATTGT GGGGAATATC 
CAAGCCACAT CTCCATGTTT ACATCCCACT GACCTCCAGA AAGTTGCAGA AATGATCCGA 
GAAGAAGGAT ATGACTCTGT CTTCTCCGTT GTGAGGCGCC ATCAGTTTCG ATGGAGTGAA 
ATTCAGAAAG GAGTTCGTGA AGTGACTGAG CCTCTGAACT TGAATCCAGC GAAACGGCCT 
CGTCGACAAG ACTGGGATGG AGAGTTATAT GAGAACGGCT CATTTTATTT TGCTAAAAGA 
CATTTGATAG AGATGGGTTA CTTACAGGGT GGGAAAATGG CATATTATGA AATGCGAGCT 
GAGCACAGTG TGGATATCGA CGTGGACATC GATTGGCCGA TCGCAGAGCA AAGAGTTCTG 
AGATTTGGCT ATTTTGGAAA AGAGAAGCTG AAGGAGATAA AGCTTTTGGT TTGTAATATT 
GATGGATGTC TCACCAATGG CCACATTTAT GTATCAGGAG ACCAAAAAGA AATAATATCT 
TATGATGTAA AAGACGCTAT TGGCATAAGT TTATTAAAGA AAAGCGGTAT TGAGGTGAGG 
CTCATCTCAG AACGGGCCTG CTCCAAGCAG ACGCTCTCTG CCCTAAAGCT GGACTGTAAA 
ACAGAAGTCA GTGTGTCCGA TAAGCTGGCC ACCGTGGATG AGTGGAGGAA GGAGATGGGC 
CTGTGCTGGA AAGAAGTGGC CTATCTCGGC AATGAAGTGT CTGATGAAGA ATGCCTCAAG 
AGAGTGGGCC TGAGCGCTGT TCCTGCCGAC GCCTGCTCCG GGGCCCAGAA GGCTGTGGGG 
TACATCTGCA AATGCAGCGG TGGCCGGGGA GCCATCCGCG AGTTTGCAGA GCACATTTTC 
CTACTGATAG AAAAAGTTAA TAACTCATGC CAAAAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_034038.2
CDS	88..1386
Translation	MDALEKGAATSGPAPRGRPSRGRPPKLQRSRGAGRGLEKPPHLAALVLARGGSKGIPLKNIKRLAGVPLIGWVLRAALDAGVFQSVWVSTDHDEIENVAKQFGAQVHRRSSETSKDSSTSLDAIVEFLNYHNEVDIVGNIQATSPCLHPTDLQKVAEMIREEGYDSVFSVVRRHQFRWSEIQKGVREVTEPLNLNPAKRPRRQDWDGELYENGSFYFAKRHLIEMGYLQGGKMAYYEMRAEHSVDIDVDIDWPIAEQRVLRFGYFGKEKLKEIKLLVCNIDGCLTNGHIYVSGDQKEIISYDVKDAIGISLLKKSGIEVRLISERACSKQTLSALKLDCKTEVSVSDKLATVDEWRKEMGLCWKEVAYLGNEVSDEECLKRVGLSAVPADACSGAQKAVGYICKCSGGRGAIREFAEHIFLLIEKVNNSCQK

Target ORF information:

RefSeq Version	NM_009908.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus cytidine monophospho-N-acetylneuraminic acid synthetase (Cmas), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_009908.2

ORF Insert Sequence:

ATGGACGCGC TGGAGAAGGG GGCCGCCACG TCGGGGCCCG CCCCGCGTGG ACGGCCGTCC 
CGGGGCCGGC CCCCGAAGCT GCAGCGCAGC CGGGGCGCGG GGCGCGGCCT AGAGAAGCCG 
CCGCACCTGG CAGCGCTGGT GCTGGCCCGC GGCGGCAGCA AAGGCATCCC ACTGAAGAAC 
ATCAAGCGCC TGGCGGGGGT TCCGCTCATT GGCTGGGTCC TGCGCGCCGC CCTGGATGCG 
GGGGTCTTCC AGAGTGTGTG GGTTTCAACA GACCATGATG AAATTGAGAA TGTGGCCAAA 
CAGTTTGGTG CACAGGTCCA TCGAAGAAGT TCTGAAACGT CCAAAGACAG CTCTACCTCA 
CTAGACGCCA TTGTAGAATT CCTGAATTAT CACAATGAGG TTGACATTGT GGGGAATATC 
CAAGCCACAT CTCCATGTTT ACATCCCACT GACCTCCAGA AAGTTGCAGA AATGATCCGA 
GAAGAAGGAT ATGACTCTGT CTTCTCCGTT GTGAGGCGCC ATCAGTTTCG ATGGAGTGAA 
ATTCAGAAAG GAGTTCGTGA AGTGACTGAG CCTCTGAACT TGAATCCAGC GAAACGGCCT 
CGTCGACAAG ACTGGGATGG AGAGTTATAT GAGAACGGCT CATTTTATTT TGCTAAAAGA 
CATTTGATAG AGATGGGTTA CTTACAGGGT GGGAAAATGG CATATTATGA AATGCGAGCT 
GAGCACAGTG TGGATATCGA CGTGGACATC GATTGGCCGA TCGCAGAGCA AAGAGTTCTG 
AGATTTGGCT ATTTTGGAAA AGAGAAGCTG AAGGAGATAA AGCTTTTGGT TTGTAATATT 
GATGGATGTC TCACCAATGG CCACATTTAT GTATCAGGAG ACCAAAAAGA AATAATATCT 
TATGATGTAA AAGACGCTAT TGGCATAAGT TTATTAAAGA AAAGCGGTAT TGAGGTGAGG 
CTCATCTCAG AACGGGCCTG CTCCAAGCAG ACGCTCTCTG CCCTAAAGCT GGACTGTAAA 
ACAGAAGTCA GTGTGTCCGA TAAGCTGGCC ACCGTGGATG AGTGGAGGAA GGAGATGGGC 
CTGTGCTGGA AAGAAGTGGC CTATCTCGGC AATGAAGTGT CTGATGAAGA ATGCCTCAAG 
AGAGTGGGCC TGAGCGCTGT TCCTGCCGAC GCCTGCTCCG GGGCCCAGAA GGCTGTGGGG 
TACATCTGCA AATGCAGCGG TGGCCGGGGA GCCATCCGCG AGTTTGCAGA GCACATTTTC 
CTACTGATAG AAAAAGTTAA TAACTCATGC CAAAAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Sialylation Is Dispensable for Early Murine Embryonic Development in Vitro.
Chembiochem : a European journal of chemical biology18(13)1305-1316(2017 07)
Abeln M,Borst KM,Cajic S,Thiesler H,Kats E,Albers I,Kuhn M,Kaever V,Rapp E,M?nster-K?hnel A,Weinhold B

A C-terminal phosphatase module conserved in vertebrate CMP-sialic acid synthetases provides a tetramerization interface for the physiologically active enzyme.
Journal of molecular biology393(1)83-97(2009 Oct)
Oschlies M,Dickmanns A,Haselhorst T,Schaper W,Stummeyer K,Tiralongo J,Weinhold B,Gerardy-Schahn R,von Itzstein M,Ficner R,M?nster-K?hnel AK

The rainbow trout CMP-sialic acid synthetase utilises a nuclear localization signal different from that identified in the mouse enzyme.
Glycobiology17(9)945-54(2007 Sep)
Tiralongo J,Fujita A,Sato C,Kitajima K,Lehmann F,Oschlies M,Gerardy-Schahn R,M?nster-K?hnel AK

Identification of the nuclear export signals that regulate the intracellular localization of the mouse CMP-sialic acid synthetase.
Biochemical and biophysical research communications355(1)174-80(2007 Mar)
Fujita A,Sato C,Kitajima K