Clcnka cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Clcnka
Entrez Gene ID	12733
Full Name	chloride channel, voltage-sensitive Ka
Synonyms	C75963,CLC-K1,Clcnk1
General protein information	Preferred Names chloride channel, voltage-sensitive Ka Names chloride channel protein ClC-Ka chloride channel K1 chloride channel Ka putative basolateral cTAL chloride channel ClC-Ka
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 4 Map Location: 4\|4 D3
Summary	This gene is a member of the CLC family of voltage-gated chloride channels. The gene is located adjacent to a highly similar chloride channel gene on chromosome 4. This gene is syntenic with human CLCNKB (geneID:1188). Multiple alternatively spliced variants, encoding the same protein, have been found for this gene. [provided by RefSeq, Sep 2009].

mRNA and Protein(s)

mRNA	Protein	Name
NM_024412.3	NP_077723.3	chloride channel protein ClC-Ka
NM_001146307.1	NP_001139779.1	chloride channel protein ClC-Ka

Pathways from BioSystems

Homologies

	CLCNKB	XP_001091784.2
Canis lupus familiaris (dog)	CLCNKA	XP_544547.2
Bos taurus (cattle)	CLCNKA	XP_005203433.1
Rattus norvegicus (Norway rat)	Clcnka	NP_445779.1
Homo sapiens (human)	CLCNKB	NP_000076.2
Mus musculus (house mouse)	Clcnka	NP_001139779.1
Pan troglodytes (chimpanzee)	CLCNKB	XP_003949353.1
Gallus gallus (chicken)	CLCNKB	XP_004947529.1
Danio rerio (zebrafish)	clcnk	NP_956676.1

Gene Ontology
Bibliography

Related articles in PubMed

Role of ClC-K and barttin in low-potassium induced sodium-chloride cotransporter activation and hypertension in mouse kidney.
Nomura N, Shoda W, Wang Y, Mandai S, Furusho T, Takahashi D, Zeniya M, Sohara E, Rai T, Uchida S
Bioscience reports(2018 Jan)

The ClC-K2 Chloride Channel Is Critical for Salt Handling in the Distal Nephron.
Hennings JC, Andrini O, Picard N, Paulais M, Huebner AK, Cayuqueo IK, Bignon Y, Keck M, Corni?re N, B?hm D, Jentsch TJ, Chambrey R, Teulon J, H?bner CA, Eladari D
Journal of the American Society of Nephrology : JASN28(1)209-217(2017 Jan)

Role of Prox1 in the Transforming Ascending Thin Limb of Henle's Loop during Mouse Kidney Development.
Kim YM, Kim WY, Nam SA, Choi AR, Kim H, Kim YK, Kim HS, Kim J
PloS one10(5)e0127429(2015)

Characterization of the mouse ClC-K1/Barttin chloride channel.
L'Hoste S, Diakov A, Andrini O, Genete M, Pinelli L, Grand T, Keck M, Paulais M, Beck L, Korbmacher C, Teulon J, Lourdel S
Biochimica et biophysica acta1828(11)2399-409(2013 Nov)

Genetic dissection of sodium and potassium transport along the aldosterone-sensitive distal nephron: importance in the control of blood pressure and hypertension.
Rossier BC, Staub O, Hummler E
FEBS letters587(13)1929-41(2013 Jun)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

molecular substrate of a chloride channel previously detected in the mouse thick ascending limb
Title: Characterization of the mouse ClC-K1/Barttin chloride channel.

induction of SGK1, CLC-K1 and barttin by high osmolarity and change in intracellular volume in distal renal tubular cells in vivo and in vitro
Title: High osmolality induces the kidney-specific chloride channel CLC-K1 by a serum and glucocorticoid-inducible kinase 1 MAPK pathway.

Gene expression profiling in the collecting tubules of aquaporin-1 and CLC-Nk1 knockout mice.
Title: Altered expression profile of transporters in the inner medullary collecting duct of aquaporin-1 knockout mice.

the major component of Cl(-) transport sensitive to NPPB or pH is mediated by CLC-K1 in the tAL.
Title: Analysis of NaCl transport in thin ascending limb of Henle's loop in CLC-K1 null mice.

The following Clcnka gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Clcnka cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu07294	NM_024412.3 Latest version!	Mus musculus chloride channel, voltage-sensitive Ka (Clcnka), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$405.30 $579.00
OMu07294	NM_001146307.1 Latest version!	Mus musculus chloride channel, voltage-sensitive Ka (Clcnka), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$405.30 $579.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Clcnka ( NM_024412.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_077723.3 Mus musculus chloride channel, voltage-sensitive Ka (Clcnka), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OMu07294
Clone ID Related Accession (Same CDS sequence)	NM_024412.3 , NM_001146307.1
Accession Version	NM_024412.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2064bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-19
Organism	Mus musculus(house mouse)
Product	chloride channel protein ClC-Ka
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from CJ068439.1, AY373832.1, BB743618.1 and BG061970.1. On Apr 7, 2009 this sequence version replaced NM_024412.2. Transcript Variant: This variant (1) represents the longer transcript. Variants 1 and 2 encode the same protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC037077.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END##

ATGGAAGAGC TGGTGGGGCT GCGTGAGGGC TCCTCCGGGA AGCCGGTGAC TCTGCAAGAG 
CTATGGGGTC CGTGTCCACG CATCCGCCGA GGCATCAGAG GGGGCCTAGA GTGGCTGAAA 
GAGCGGCTGT TCCGTGCGCG TGAGGACTGG TACTTCCTGG TGGCTCTTGG GGTGCTCATG 
GCTCTGATCA GCTATGCCAT GAACTTTGCT ATCGGGCGTG TGGTCAGAGC ACACAAGTGG 
CTGTACAGAG AAGTTGGGGA CGGCCACCTG CTCCGGTACC TCTCCTGGAC CGTGTACCCC 
GTAGCTCTTC TGTCCTTCTC CTCCGGCTTC TCACAGAGCA TTACGCCCTT CTCTGGAGGG 
TCTGGACTCC CAGAGCTGAA GACCATGCTG TCTGGAGTAG TCCTGGAGAA CTACCTAGAC 
ATCAAGAACT TCGGGGCCAA GGTGGTGGGC CTCTCCTGCA CCCTGGCCAC AGGCAGCACC 
ATCTTCCTAG GCAAAGTGGG CCCGTTCGTG CACCTGAGTG TGATGATTTC TGCTTATCTG 
GGCCGCGTGC GCGCCAAAGC CGTCGGGGAT ACTGAGAGCA AGGCCAAGGA AGTTGAAATG 
CTGTCGGCGG CAGCGGCGGT GGGCGTGGCC ACAGTCTTCG CCGCCCCCTT CAGTGGTGTC 
CTGTTCAGCA TCGAGGTCAT GTCCTCTCAC TTCTCCGTCT GGAATTACTG GAGGGGCTTC 
TTCGCTGCCA CCTGTGGGGC CTTCATGTTC CGTCTCCTGG CGGTCTTCAA CAGTGAACAG 
GAGACCATCA CCTCCATCTA CAAGACCAGA TTCCGAGTGG ATGTGCCCTT TGACCTGCCT 
GAAATCTTCT TCTTTGTGGC TCTGGGGTTC ATCTGCGGGA TCCTCAGTTG CGTTTACCTG 
TTCTGTCAGC GAAATTTTCT CCGCTTCATC AAGACCAATC GCTACACCTC CAAACTGCTG 
GCTACAAGCA AGCCTTCCTA CGCAGCTCTG GTCGCGCTGG TCCTGGCTTC AGTCACCTAT 
CCTCCTGGTG TGGGCCGTTT CATGGCTTCC CGGCTGTCCA TGGCGGAACA CTTGCACTCT 
CTGTTTGACA ACAACTCGTG GGCGTTGATG ACTCGGAATG CGTCCCCACC CTGGCCTTCT 
GAGCCCGACC CCCAGAACCT GTGGTTTGAA TGGTACCACC CACGGTTCAC CATCTTTGGG 
ACGCTGGCCT TCTTCCTGAT CATGAAGTTC TGGATGCTGA TTCTGGCCAC CACGATCCCC 
ATGCCTGCAG GCTACTTCAT GCCTATATTC ATCATCGGAG CTGCCATCGG GCGCCTCTTG 
GGAGAGGCCC TGTCTGTCGC CTTCCCAGAG GGCATTGTGG CTGGCGGAGA GGTCTATCCC 
ATCATGCCTG GGGGCTATGC TCTGGCAGGT GCTGCTGCCT TCTCCGGAGC AGTGACCCAC 
ACCATCTCCA CAGCGCTGCT GGCCTTTGAG CTGACTGGCC AGATCGTTCA CGCACTGCCT 
GTGCTGATGG CTGTGCTGGC GGCCAATGTC ATCTCCCAAA ACTTACAGCC CTCTTTCTAC 
GATGGCACCA TCATGGCCAA GAAACTGCCG TACCTGCCGT GGATCCGTGG CCGACAGATT 
GGCTCCTACC CTGTAACGGT GGAACATTTC ATGAACTCTA ACCTCACCAC ACTGGCCAAG 
GACATGCCCC TGGAGGAGGT GGTCAAGGTG GTGACCTCCA CAGATGTGTC TCAGTATCCC 
TTGGTGGAGA CCAGAGAGTC TCAGACCCTG GTGGGCATTG TGGAAAGGAC CCACTTGGTG 
CAAGCCCTTC AGACCCAGCC AGCTTCCTGG GCTCCAGGCC AAGAGCGATT TCTCCAGGAC 
ATCTTGGCTG GTGGCTGCCC CACACAGCCA GTGACCCTGC AGCTGTCCCC AGAGACCTCC 
CTATATCAGG CACACAGGCT CTTTGAGCTG CTGACCCTTC AGGCGCTGTT CGTGACGTCA 
CGGGGCAGAG CTGTGGGCTC TGTGTCCTGG GTGGAGCTGA AGAAAGCCAT TTCCACCTTG 
ATCAACCCAC CAGCCCCCAA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_077723.3
CDS	56..2119
Translation	MEELVGLREGSSGKPVTLQELWGPCPRIRRGIRGGLEWLKERLFRAREDWYFLVALGVLMALISYAMNFAIGRVVRAHKWLYREVGDGHLLRYLSWTVYPVALLSFSSGFSQSITPFSGGSGLPELKTMLSGVVLENYLDIKNFGAKVVGLSCTLATGSTIFLGKVGPFVHLSVMISAYLGRVRAKAVGDTESKAKEVEMLSAAAAVGVATVFAAPFSGVLFSIEVMSSHFSVWNYWRGFFAATCGAFMFRLLAVFNSEQETITSIYKTRFRVDVPFDLPEIFFFVALGFICGILSCVYLFCQRNFLRFIKTNRYTSKLLATSKPSYAALVALVLASVTYPPGVGRFMASRLSMAEHLHSLFDNNSWALMTRNASPPWPSEPDPQNLWFEWYHPRFTIFGTLAFFLIMKFWMLILATTIPMPAGYFMPIFIIGAAIGRLLGEALSVAFPEGIVAGGEVYPIMPGGYALAGAAAFSGAVTHTISTALLAFELTGQIVHALPVLMAVLAANVISQNLQPSFYDGTIMAKKLPYLPWIRGRQIGSYPVTVEHFMNSNLTTLAKDMPLEEVVKVVTSTDVSQYPLVETRESQTLVGIVERTHLVQALQTQPASWAPGQERFLQDILAGGCPTQPVTLQLSPETSLYQAHRLFELLTLQALFVTSRGRAVGSVSWVELKKAISTLINPPAPK

Target ORF information:

RefSeq Version	NM_024412.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus chloride channel, voltage-sensitive Ka (Clcnka), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_024412.3

ORF Insert Sequence:

ATGGAAGAGC TGGTGGGGCT GCGTGAGGGC TCCTCCGGGA AGCCGGTGAC TCTGCAAGAG 
CTATGGGGTC CGTGTCCACG CATCCGCCGA GGCATCAGAG GGGGCCTAGA GTGGCTGAAA 
GAGCGGCTGT TCCGTGCGCG TGAGGACTGG TACTTCCTGG TGGCTCTTGG GGTGCTCATG 
GCTCTGATCA GCTATGCCAT GAACTTTGCT ATCGGGCGTG TGGTCAGAGC ACACAAGTGG 
CTGTACAGAG AAGTTGGGGA CGGCCACCTG CTCCGGTACC TCTCCTGGAC CGTGTACCCC 
GTAGCTCTTC TGTCCTTCTC CTCCGGCTTC TCACAGAGCA TTACGCCCTT CTCTGGAGGG 
TCTGGACTCC CAGAGCTGAA GACCATGCTG TCTGGAGTAG TCCTGGAGAA CTACCTAGAC 
ATCAAGAACT TCGGGGCCAA GGTGGTGGGC CTCTCCTGCA CCCTGGCCAC AGGCAGCACC 
ATCTTCCTAG GCAAAGTGGG CCCGTTCGTG CACCTGAGTG TGATGATTTC TGCTTATCTG 
GGCCGCGTGC GCGCCAAAGC CGTCGGGGAT ACTGAGAGCA AGGCCAAGGA AGTTGAAATG 
CTGTCGGCGG CAGCGGCGGT GGGCGTGGCC ACAGTCTTCG CCGCCCCCTT CAGTGGTGTC 
CTGTTCAGCA TCGAGGTCAT GTCCTCTCAC TTCTCCGTCT GGAATTACTG GAGGGGCTTC 
TTCGCTGCCA CCTGTGGGGC CTTCATGTTC CGTCTCCTGG CGGTCTTCAA CAGTGAACAG 
GAGACCATCA CCTCCATCTA CAAGACCAGA TTCCGAGTGG ATGTGCCCTT TGACCTGCCT 
GAAATCTTCT TCTTTGTGGC TCTGGGGTTC ATCTGCGGGA TCCTCAGTTG CGTTTACCTG 
TTCTGTCAGC GAAATTTTCT CCGCTTCATC AAGACCAATC GCTACACCTC CAAACTGCTG 
GCTACAAGCA AGCCTTCCTA CGCAGCTCTG GTCGCGCTGG TCCTGGCTTC AGTCACCTAT 
CCTCCTGGTG TGGGCCGTTT CATGGCTTCC CGGCTGTCCA TGGCGGAACA CTTGCACTCT 
CTGTTTGACA ACAACTCGTG GGCGTTGATG ACTCGGAATG CGTCCCCACC CTGGCCTTCT 
GAGCCCGACC CCCAGAACCT GTGGTTTGAA TGGTACCACC CACGGTTCAC CATCTTTGGG 
ACGCTGGCCT TCTTCCTGAT CATGAAGTTC TGGATGCTGA TTCTGGCCAC CACGATCCCC 
ATGCCTGCAG GCTACTTCAT GCCTATATTC ATCATCGGAG CTGCCATCGG GCGCCTCTTG 
GGAGAGGCCC TGTCTGTCGC CTTCCCAGAG GGCATTGTGG CTGGCGGAGA GGTCTATCCC 
ATCATGCCTG GGGGCTATGC TCTGGCAGGT GCTGCTGCCT TCTCCGGAGC AGTGACCCAC 
ACCATCTCCA CAGCGCTGCT GGCCTTTGAG CTGACTGGCC AGATCGTTCA CGCACTGCCT 
GTGCTGATGG CTGTGCTGGC GGCCAATGTC ATCTCCCAAA ACTTACAGCC CTCTTTCTAC 
GATGGCACCA TCATGGCCAA GAAACTGCCG TACCTGCCGT GGATCCGTGG CCGACAGATT 
GGCTCCTACC CTGTAACGGT GGAACATTTC ATGAACTCTA ACCTCACCAC ACTGGCCAAG 
GACATGCCCC TGGAGGAGGT GGTCAAGGTG GTGACCTCCA CAGATGTGTC TCAGTATCCC 
TTGGTGGAGA CCAGAGAGTC TCAGACCCTG GTGGGCATTG TGGAAAGGAC CCACTTGGTG 
CAAGCCCTTC AGACCCAGCC AGCTTCCTGG GCTCCAGGCC AAGAGCGATT TCTCCAGGAC 
ATCTTGGCTG GTGGCTGCCC CACACAGCCA GTGACCCTGC AGCTGTCCCC AGAGACCTCC 
CTATATCAGG CACACAGGCT CTTTGAGCTG CTGACCCTTC AGGCGCTGTT CGTGACGTCA 
CGGGGCAGAG CTGTGGGCTC TGTGTCCTGG GTGGAGCTGA AGAAAGCCAT TTCCACCTTG 
ATCAACCCAC CAGCCCCCAA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Clcnka ( NM_001146307.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001139779.1 Mus musculus chloride channel, voltage-sensitive Ka (Clcnka), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OMu07294
Clone ID Related Accession (Same CDS sequence)	NM_024412.3 , NM_001146307.1
Accession Version	NM_001146307.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2064bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-19
Organism	Mus musculus(house mouse)
Product	chloride channel protein ClC-Ka
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from CF725377.1, AY373832.1, BB743618.1 and BG061970.1. Transcript Variant: This variant (2) differs in the 5' UTR, compared to variant 1. Variants 1 and 2 encode the same protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF124848.1, AY373832.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END##

ATGGAAGAGC TGGTGGGGCT GCGTGAGGGC TCCTCCGGGA AGCCGGTGAC TCTGCAAGAG 
CTATGGGGTC CGTGTCCACG CATCCGCCGA GGCATCAGAG GGGGCCTAGA GTGGCTGAAA 
GAGCGGCTGT TCCGTGCGCG TGAGGACTGG TACTTCCTGG TGGCTCTTGG GGTGCTCATG 
GCTCTGATCA GCTATGCCAT GAACTTTGCT ATCGGGCGTG TGGTCAGAGC ACACAAGTGG 
CTGTACAGAG AAGTTGGGGA CGGCCACCTG CTCCGGTACC TCTCCTGGAC CGTGTACCCC 
GTAGCTCTTC TGTCCTTCTC CTCCGGCTTC TCACAGAGCA TTACGCCCTT CTCTGGAGGG 
TCTGGACTCC CAGAGCTGAA GACCATGCTG TCTGGAGTAG TCCTGGAGAA CTACCTAGAC 
ATCAAGAACT TCGGGGCCAA GGTGGTGGGC CTCTCCTGCA CCCTGGCCAC AGGCAGCACC 
ATCTTCCTAG GCAAAGTGGG CCCGTTCGTG CACCTGAGTG TGATGATTTC TGCTTATCTG 
GGCCGCGTGC GCGCCAAAGC CGTCGGGGAT ACTGAGAGCA AGGCCAAGGA AGTTGAAATG 
CTGTCGGCGG CAGCGGCGGT GGGCGTGGCC ACAGTCTTCG CCGCCCCCTT CAGTGGTGTC 
CTGTTCAGCA TCGAGGTCAT GTCCTCTCAC TTCTCCGTCT GGAATTACTG GAGGGGCTTC 
TTCGCTGCCA CCTGTGGGGC CTTCATGTTC CGTCTCCTGG CGGTCTTCAA CAGTGAACAG 
GAGACCATCA CCTCCATCTA CAAGACCAGA TTCCGAGTGG ATGTGCCCTT TGACCTGCCT 
GAAATCTTCT TCTTTGTGGC TCTGGGGTTC ATCTGCGGGA TCCTCAGTTG CGTTTACCTG 
TTCTGTCAGC GAAATTTTCT CCGCTTCATC AAGACCAATC GCTACACCTC CAAACTGCTG 
GCTACAAGCA AGCCTTCCTA CGCAGCTCTG GTCGCGCTGG TCCTGGCTTC AGTCACCTAT 
CCTCCTGGTG TGGGCCGTTT CATGGCTTCC CGGCTGTCCA TGGCGGAACA CTTGCACTCT 
CTGTTTGACA ACAACTCGTG GGCGTTGATG ACTCGGAATG CGTCCCCACC CTGGCCTTCT 
GAGCCCGACC CCCAGAACCT GTGGTTTGAA TGGTACCACC CACGGTTCAC CATCTTTGGG 
ACGCTGGCCT TCTTCCTGAT CATGAAGTTC TGGATGCTGA TTCTGGCCAC CACGATCCCC 
ATGCCTGCAG GCTACTTCAT GCCTATATTC ATCATCGGAG CTGCCATCGG GCGCCTCTTG 
GGAGAGGCCC TGTCTGTCGC CTTCCCAGAG GGCATTGTGG CTGGCGGAGA GGTCTATCCC 
ATCATGCCTG GGGGCTATGC TCTGGCAGGT GCTGCTGCCT TCTCCGGAGC AGTGACCCAC 
ACCATCTCCA CAGCGCTGCT GGCCTTTGAG CTGACTGGCC AGATCGTTCA CGCACTGCCT 
GTGCTGATGG CTGTGCTGGC GGCCAATGTC ATCTCCCAAA ACTTACAGCC CTCTTTCTAC 
GATGGCACCA TCATGGCCAA GAAACTGCCG TACCTGCCGT GGATCCGTGG CCGACAGATT 
GGCTCCTACC CTGTAACGGT GGAACATTTC ATGAACTCTA ACCTCACCAC ACTGGCCAAG 
GACATGCCCC TGGAGGAGGT GGTCAAGGTG GTGACCTCCA CAGATGTGTC TCAGTATCCC 
TTGGTGGAGA CCAGAGAGTC TCAGACCCTG GTGGGCATTG TGGAAAGGAC CCACTTGGTG 
CAAGCCCTTC AGACCCAGCC AGCTTCCTGG GCTCCAGGCC AAGAGCGATT TCTCCAGGAC 
ATCTTGGCTG GTGGCTGCCC CACACAGCCA GTGACCCTGC AGCTGTCCCC AGAGACCTCC 
CTATATCAGG CACACAGGCT CTTTGAGCTG CTGACCCTTC AGGCGCTGTT CGTGACGTCA 
CGGGGCAGAG CTGTGGGCTC TGTGTCCTGG GTGGAGCTGA AGAAAGCCAT TTCCACCTTG 
ATCAACCCAC CAGCCCCCAA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001139779.1
CDS	52..2115
Translation	MEELVGLREGSSGKPVTLQELWGPCPRIRRGIRGGLEWLKERLFRAREDWYFLVALGVLMALISYAMNFAIGRVVRAHKWLYREVGDGHLLRYLSWTVYPVALLSFSSGFSQSITPFSGGSGLPELKTMLSGVVLENYLDIKNFGAKVVGLSCTLATGSTIFLGKVGPFVHLSVMISAYLGRVRAKAVGDTESKAKEVEMLSAAAAVGVATVFAAPFSGVLFSIEVMSSHFSVWNYWRGFFAATCGAFMFRLLAVFNSEQETITSIYKTRFRVDVPFDLPEIFFFVALGFICGILSCVYLFCQRNFLRFIKTNRYTSKLLATSKPSYAALVALVLASVTYPPGVGRFMASRLSMAEHLHSLFDNNSWALMTRNASPPWPSEPDPQNLWFEWYHPRFTIFGTLAFFLIMKFWMLILATTIPMPAGYFMPIFIIGAAIGRLLGEALSVAFPEGIVAGGEVYPIMPGGYALAGAAAFSGAVTHTISTALLAFELTGQIVHALPVLMAVLAANVISQNLQPSFYDGTIMAKKLPYLPWIRGRQIGSYPVTVEHFMNSNLTTLAKDMPLEEVVKVVTSTDVSQYPLVETRESQTLVGIVERTHLVQALQTQPASWAPGQERFLQDILAGGCPTQPVTLQLSPETSLYQAHRLFELLTLQALFVTSRGRAVGSVSWVELKKAISTLINPPAPK

Target ORF information:

RefSeq Version	NM_001146307.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus chloride channel, voltage-sensitive Ka (Clcnka), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001146307.1

ORF Insert Sequence:

ATGGAAGAGC TGGTGGGGCT GCGTGAGGGC TCCTCCGGGA AGCCGGTGAC TCTGCAAGAG 
CTATGGGGTC CGTGTCCACG CATCCGCCGA GGCATCAGAG GGGGCCTAGA GTGGCTGAAA 
GAGCGGCTGT TCCGTGCGCG TGAGGACTGG TACTTCCTGG TGGCTCTTGG GGTGCTCATG 
GCTCTGATCA GCTATGCCAT GAACTTTGCT ATCGGGCGTG TGGTCAGAGC ACACAAGTGG 
CTGTACAGAG AAGTTGGGGA CGGCCACCTG CTCCGGTACC TCTCCTGGAC CGTGTACCCC 
GTAGCTCTTC TGTCCTTCTC CTCCGGCTTC TCACAGAGCA TTACGCCCTT CTCTGGAGGG 
TCTGGACTCC CAGAGCTGAA GACCATGCTG TCTGGAGTAG TCCTGGAGAA CTACCTAGAC 
ATCAAGAACT TCGGGGCCAA GGTGGTGGGC CTCTCCTGCA CCCTGGCCAC AGGCAGCACC 
ATCTTCCTAG GCAAAGTGGG CCCGTTCGTG CACCTGAGTG TGATGATTTC TGCTTATCTG 
GGCCGCGTGC GCGCCAAAGC CGTCGGGGAT ACTGAGAGCA AGGCCAAGGA AGTTGAAATG 
CTGTCGGCGG CAGCGGCGGT GGGCGTGGCC ACAGTCTTCG CCGCCCCCTT CAGTGGTGTC 
CTGTTCAGCA TCGAGGTCAT GTCCTCTCAC TTCTCCGTCT GGAATTACTG GAGGGGCTTC 
TTCGCTGCCA CCTGTGGGGC CTTCATGTTC CGTCTCCTGG CGGTCTTCAA CAGTGAACAG 
GAGACCATCA CCTCCATCTA CAAGACCAGA TTCCGAGTGG ATGTGCCCTT TGACCTGCCT 
GAAATCTTCT TCTTTGTGGC TCTGGGGTTC ATCTGCGGGA TCCTCAGTTG CGTTTACCTG 
TTCTGTCAGC GAAATTTTCT CCGCTTCATC AAGACCAATC GCTACACCTC CAAACTGCTG 
GCTACAAGCA AGCCTTCCTA CGCAGCTCTG GTCGCGCTGG TCCTGGCTTC AGTCACCTAT 
CCTCCTGGTG TGGGCCGTTT CATGGCTTCC CGGCTGTCCA TGGCGGAACA CTTGCACTCT 
CTGTTTGACA ACAACTCGTG GGCGTTGATG ACTCGGAATG CGTCCCCACC CTGGCCTTCT 
GAGCCCGACC CCCAGAACCT GTGGTTTGAA TGGTACCACC CACGGTTCAC CATCTTTGGG 
ACGCTGGCCT TCTTCCTGAT CATGAAGTTC TGGATGCTGA TTCTGGCCAC CACGATCCCC 
ATGCCTGCAG GCTACTTCAT GCCTATATTC ATCATCGGAG CTGCCATCGG GCGCCTCTTG 
GGAGAGGCCC TGTCTGTCGC CTTCCCAGAG GGCATTGTGG CTGGCGGAGA GGTCTATCCC 
ATCATGCCTG GGGGCTATGC TCTGGCAGGT GCTGCTGCCT TCTCCGGAGC AGTGACCCAC 
ACCATCTCCA CAGCGCTGCT GGCCTTTGAG CTGACTGGCC AGATCGTTCA CGCACTGCCT 
GTGCTGATGG CTGTGCTGGC GGCCAATGTC ATCTCCCAAA ACTTACAGCC CTCTTTCTAC 
GATGGCACCA TCATGGCCAA GAAACTGCCG TACCTGCCGT GGATCCGTGG CCGACAGATT 
GGCTCCTACC CTGTAACGGT GGAACATTTC ATGAACTCTA ACCTCACCAC ACTGGCCAAG 
GACATGCCCC TGGAGGAGGT GGTCAAGGTG GTGACCTCCA CAGATGTGTC TCAGTATCCC 
TTGGTGGAGA CCAGAGAGTC TCAGACCCTG GTGGGCATTG TGGAAAGGAC CCACTTGGTG 
CAAGCCCTTC AGACCCAGCC AGCTTCCTGG GCTCCAGGCC AAGAGCGATT TCTCCAGGAC 
ATCTTGGCTG GTGGCTGCCC CACACAGCCA GTGACCCTGC AGCTGTCCCC AGAGACCTCC 
CTATATCAGG CACACAGGCT CTTTGAGCTG CTGACCCTTC AGGCGCTGTT CGTGACGTCA 
CGGGGCAGAG CTGTGGGCTC TGTGTCCTGG GTGGAGCTGA AGAAAGCCAT TTCCACCTTG 
ATCAACCCAC CAGCCCCCAA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Role of ClC-K and barttin in low-potassium induced sodium-chloride cotransporter activation and hypertension in mouse kidney.
Bioscience reports(2018 Jan)
Nomura N,Shoda W,Wang Y,Mandai S,Furusho T,Takahashi D,Zeniya M,Sohara E,Rai T,Uchida S

The ClC-K2 Chloride Channel Is Critical for Salt Handling in the Distal Nephron.
Journal of the American Society of Nephrology : JASN28(1)209-217(2017 Jan)
Hennings JC,Andrini O,Picard N,Paulais M,Huebner AK,Cayuqueo IK,Bignon Y,Keck M,Corni?re N,B?hm D,Jentsch TJ,Chambrey R,Teulon J,H?bner CA,Eladari D

Role of Prox1 in the Transforming Ascending Thin Limb of Henle's Loop during Mouse Kidney Development.
PloS one10(5)e0127429(2015)
Kim YM,Kim WY,Nam SA,Choi AR,Kim H,Kim YK,Kim HS,Kim J

Characterization of the mouse ClC-K1/Barttin chloride channel.
Biochimica et biophysica acta1828(11)2399-409(2013 Nov)
L'Hoste S,Diakov A,Andrini O,Genete M,Pinelli L,Grand T,Keck M,Paulais M,Beck L,Korbmacher C,Teulon J,Lourdel S

Genetic dissection of sodium and potassium transport along the aldosterone-sensitive distal nephron: importance in the control of blood pressure and hypertension.
FEBS letters587(13)1929-41(2013 Jun)
Rossier BC,Staub O,Hummler E