Cit cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Cit
Entrez Gene ID	12704
Full Name	citron
Synonyms	C030025P15Rik,CRIK,CRIK-SK,Cit-k
General protein information	Preferred Names citron Names citron Rho-interacting kinase citron kinase citron-N rho-interacting, serine/threonine-protein kinase 21
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 5 Map Location: 5\|5 F

mRNA and Protein(s)

mRNA	Protein	Name
XM_006530141.2	XP_006530204.1	citron Rho-interacting kinase isoform X1
XM_006530130.3	XP_006530193.1	citron Rho-interacting kinase isoform X1
XM_006530132.3	XP_006530195.1	citron Rho-interacting kinase isoform X1
XM_006530133.3	XP_006530196.1	citron Rho-interacting kinase isoform X1
XM_006530143.3	XP_006530206.1	citron Rho-interacting kinase isoform X11
XM_006530145.3	XP_006530208.1	citron Rho-interacting kinase isoform X13
XM_006530148.3	XP_006530211.1	citron Rho-interacting kinase isoform X16
XM_017320641.1	XP_017176130.1	citron Rho-interacting kinase isoform X19
XM_017320645.1	XP_017176134.1	citron Rho-interacting kinase isoform X23
XM_006530135.4	XP_006530198.1	citron Rho-interacting kinase isoform X3
XM_006530137.4	XP_006530200.1	citron Rho-interacting kinase isoform X5
XM_030254099.1	XP_030109959.1	citron Rho-interacting kinase isoform X7
XM_006530140.4	XP_006530203.1	citron Rho-interacting kinase isoform X10
XM_006530145.4	XP_006530208.1	citron Rho-interacting kinase isoform X15
XM_006530147.2	XP_006530210.1	citron Rho-interacting kinase isoform X18
XM_006530148.4	XP_006530211.1	citron Rho-interacting kinase isoform X18
XM_006530150.4	XP_006530213.1	citron Rho-interacting kinase isoform X20
XM_017320643.2	XP_017176132.1	citron Rho-interacting kinase isoform X22
XM_017320644.2	XP_017176133.1	citron Rho-interacting kinase isoform X23
XM_017320645.2	XP_017176134.1	citron Rho-interacting kinase isoform X24
XM_017320646.2	XP_017176135.1	citron Rho-interacting kinase isoform X25
XM_006530135.3	XP_006530198.1	citron Rho-interacting kinase isoform X3
XM_006530151.3	XP_006530214.1	citron Rho-interacting kinase isoform X26
XM_006530143.4	XP_006530206.1	citron Rho-interacting kinase isoform X13
XM_017320640.1	XP_017176129.1	citron Rho-interacting kinase isoform X14
XM_006530149.4	XP_006530212.1	citron Rho-interacting kinase isoform X19
XM_017320641.2	XP_017176130.1	citron Rho-interacting kinase isoform X21
XM_006530134.3	XP_006530197.1	citron Rho-interacting kinase isoform X2
XM_006530136.3	XP_006530199.1	citron Rho-interacting kinase isoform X4
XM_006530138.3	XP_006530201.1	citron Rho-interacting kinase isoform X6
XM_006530140.3	XP_006530203.1	citron Rho-interacting kinase isoform X8
XM_011248163.2	XP_011246465.1	citron Rho-interacting kinase isoform X14
XM_006530147.1	XP_006530210.1	citron Rho-interacting kinase isoform X16
XM_006530150.3	XP_006530213.1	citron Rho-interacting kinase isoform X18
XM_017320643.1	XP_017176132.1	citron Rho-interacting kinase isoform X21
XM_017320646.1	XP_017176135.1	citron Rho-interacting kinase isoform X24
XM_006530130.4	XP_006530193.1	citron Rho-interacting kinase isoform X1
XM_006530131.2	XP_006530194.1	citron Rho-interacting kinase isoform X1
XM_006530134.4	XP_006530197.1	citron Rho-interacting kinase isoform X2
XM_006530136.4	XP_006530199.1	citron Rho-interacting kinase isoform X4
XM_006530142.3	XP_006530205.1	citron Rho-interacting kinase isoform X12
XM_006530131.1	XP_006530194.1	citron Rho-interacting kinase isoform X1
XM_006530139.3	XP_006530202.1	citron Rho-interacting kinase isoform X7
XM_017320642.1	XP_017176131.1	citron Rho-interacting kinase isoform X20
XM_017320644.1	XP_017176133.1	citron Rho-interacting kinase isoform X22
XM_006530152.1	XP_006530215.1	citron Rho-interacting kinase isoform X27
XM_006530133.4	XP_006530196.1	citron Rho-interacting kinase isoform X1
XM_030254098.1	XP_030109958.1	citron Rho-interacting kinase isoform X6
XM_006530138.4	XP_006530201.1	citron Rho-interacting kinase isoform X7
XM_006530141.4	XP_006530204.1	citron Rho-interacting kinase isoform X11
XM_006530146.3	XP_006530209.1	citron Rho-interacting kinase isoform X17
XM_006530153.1	XP_006530216.1	citron Rho-interacting kinase isoform X28
NM_007708.3	NP_031734.3	citron Rho-interacting kinase
XM_006530137.3	XP_006530200.1	citron Rho-interacting kinase isoform X5
XM_006530141.3	XP_006530204.1	citron Rho-interacting kinase isoform X9
XM_006530149.3	XP_006530212.1	citron Rho-interacting kinase isoform X17
XM_017320647.1	XP_017176136.1	citron Rho-interacting kinase isoform X25
XM_006530132.4	XP_006530195.1	citron Rho-interacting kinase isoform X1
XM_030254100.1	XP_030109960.1	citron Rho-interacting kinase isoform X8
XM_006530139.4	XP_006530202.1	citron Rho-interacting kinase isoform X9
XM_011248163.3	XP_011246465.1	citron Rho-interacting kinase isoform X16
XM_006530151.4	XP_006530214.1	citron Rho-interacting kinase isoform X26
XM_006530152.2	XP_006530215.1	citron Rho-interacting kinase isoform X27

Pathways from BioSystems

Homologies

Homo sapiens (human)	CIT	NP_001193928.1
Pan troglodytes (chimpanzee)	CIT	XP_003952361.1
Bos taurus (cattle)	CIT	XP_002694628.3
Rattus norvegicus (Norway rat)	Cit	NP_001025082.1
Drosophila melanogaster (fruit fly)	sti	NP_001261768.1
Canis lupus familiaris (dog)	CIT	XP_003433432.1
Mus musculus (house mouse)	Cit	NP_031734.3
Danio rerio (zebrafish)	LOC100329792	XP_005171785.1
	CIT	XP_001085560.1
Gallus gallus (chicken)	CIT	XP_004934462.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP006932	XP_308829.4
Xenopus tropicalis (tropical clawed frog)	cit	NP_001120601.1

Gene Ontology
Bibliography

Related articles in PubMed

Citron Kinase Deficiency Leads to Chromosomal Instability and TP53-Sensitive Microcephaly.
Bianchi FT, Tocco C, Pallavicini G, Liu Y, Vern? F, Merigliano C, Bonaccorsi S, El-Assawy N, Priano L, Gai M, Berto GE, Chiotto AM, Sgr? F, Caramello A, Tasca L, Ala U, Neri F, Oliviero S, Mauro A, Geley S, Gatti M, Di Cunto F
Cell reports18(7)1674-1686(2017 Feb)

ASPM and CITK regulate spindle orientation by affecting the dynamics of astral microtubules.
Gai M, Bianchi FT, Vagnoni C, Vern? F, Bonaccorsi S, Pasquero S, Berto GE, Sgr? F, Chiotto AM, Annaratone L, Sapino A, Bergo A, Landsberger N, Bond J, Huttner WB, Di Cunto F
EMBO reports17(10)1396-1409(2016 10)

Tissue-specific control of midbody microtubule stability by Citron kinase through modulation of TUBB3 phosphorylation.
Sgr? F, Bianchi FT, Falcone M, Pallavicini G, Gai M, Chiotto AM, Berto GE, Turco E, Chang YJ, Huttner WB, Di Cunto F
Cell death and differentiation23(5)801-13(2016 May)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

in this study we have provided evidence that ASPM controls spindle orientation by regulating the dynamics of astral MT and that CITK is a critical downstream partner of ASPM for this activity.
Title: ASPM and CITK regulate spindle orientation by affecting the dynamics of astral microtubules.

The CIT-K may promote this event by interacting with TUBB3 and by recruiting at the midbody casein kinase-2alpha (CK2alpha) that has previously been reported to phosphorylate the S444 residue.
Title: Tissue-specific control of midbody microtubule stability by Citron kinase through modulation of TUBB3 phosphorylation.

Data propose that the CC-domain-mediated translocation and actions of Citron-K ensure proper stabilization of the midbody structure during the transition from constriction to abscission.
Title: Citron kinase mediates transition from constriction to abscission through its coiled-coil domain.

p27 has a role in cytokinesis via the regulation of citron-K activity
Title: p27(Kip1) controls cytokinesis via the regulation of citron kinase activation.

Data report that Discs large 5 (Dlg5), a member of the MAGUK family, is an interactor of CitK required for CitK polarization.
Title: Discs large 5 is required for polarization of citron kinase in mitotic neural precursors.

The following Cit gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Cit cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu35791	XM_006530152.2 Latest version!	Mus musculus citron (Cit), transcript variant X32, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OMu35792	XM_006530153.1 Latest version!	Mus musculus citron (Cit), transcript variant X33, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$643.30 $919.00
OMu35787	XM_006530148.4 Latest version!	Mus musculus citron (Cit), transcript variant X23, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OMu35787	XM_006530147.2 Latest version!	Mus musculus citron (Cit), transcript variant X22, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OMu35788	XM_006530149.4 Latest version!	Mus musculus citron (Cit), transcript variant X24, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OMu35789	XM_006530150.4 Latest version!	Mus musculus citron (Cit), transcript variant X25, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OMu35790	XM_006530151.4 Latest version!	Mus musculus citron (Cit), transcript variant X31, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OMu81232	XM_017320641.2 Latest version!	Mus musculus citron (Cit), transcript variant X26, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OMu81233	XM_017320642.1 Latest version!	Mus musculus citron (Cit), transcript variant X24, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OMu81234	XM_017320643.2 Latest version!	Mus musculus citron (Cit), transcript variant X27, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OMu81235	XM_017320644.2 Latest version!	Mus musculus citron (Cit), transcript variant X28, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OMu81236	XM_017320645.2 Latest version!	Mus musculus citron (Cit), transcript variant X29, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OMu81237	XM_017320646.2 Latest version!	Mus musculus citron (Cit), transcript variant X30, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OMu81238	XM_017320647.1 Latest version!	Mus musculus citron (Cit), transcript variant X29, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OMu35786	XM_006530146.3 Latest version!	Mus musculus citron (Cit), transcript variant X21, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
OMu00646	XM_006530141.4 Latest version!	Mus musculus citron (Cit), transcript variant X15, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OMu35774	XM_006530132.4 Latest version!	Mus musculus citron (Cit), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OMu35774	XM_006530133.4 Latest version!	Mus musculus citron (Cit), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OMu35774	XM_006530130.4 Latest version!	Mus musculus citron (Cit), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OMu35774	XM_006530131.2 Latest version!	Mus musculus citron (Cit), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OMu35775	XM_006530134.4 Latest version!	Mus musculus citron (Cit), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OMu35776	XM_006530135.4 Latest version!	Mus musculus citron (Cit), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OMu35777	XM_006530136.4 Latest version!	Mus musculus citron (Cit), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OMu35778	XM_006530137.4 Latest version!	Mus musculus citron (Cit), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OMu35779	XM_006530138.4 Latest version!	Mus musculus citron (Cit), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OMu35779	XM_030254099.1 Latest version!	Mus musculus citron (Cit), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OMu35780	XM_006530139.4 Latest version!	Mus musculus citron (Cit), transcript variant X13, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OMu35781	XM_006530140.4 Latest version!	Mus musculus citron (Cit), transcript variant X14, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OMu35783	XM_006530143.4 Latest version!	Mus musculus citron (Cit), transcript variant X17, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OMu35785	XM_006530145.4 Latest version!	Mus musculus citron (Cit), transcript variant X19, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OMu67373	XM_011248163.3 Latest version!	Mus musculus citron (Cit), transcript variant X20, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OMu81231	XM_017320640.1 Latest version!	Mus musculus citron (Cit), transcript variant X18, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OMu88722	XM_030254098.1 Latest version!	Mus musculus citron (Cit), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OMu88723	XM_030254100.1 Latest version!	Mus musculus citron (Cit), transcript variant X12, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OMu00646	NM_007708.3 Latest version!	Mus musculus citron (Cit), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1091.30 $1559.00
OMu35782	XM_006530142.3 Latest version!	Mus musculus citron (Cit), transcript variant X16, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1091.30 $1559.00
View Old Accession Versions >>
OMu35785	XM_006530145.3	Mus musculus citron (Cit), transcript variant X16, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1091.30 $1559.00
OMu35787	XM_006530148.3	Mus musculus citron (Cit), transcript variant X20, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
OMu81236	XM_017320645.1	Mus musculus citron (Cit), transcript variant X27, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OMu35776	XM_006530135.3	Mus musculus citron (Cit), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1091.30 $1559.00
OMu35783	XM_006530143.3	Mus musculus citron (Cit), transcript variant X14, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1091.30 $1559.00
OMu81232	XM_017320641.1	Mus musculus citron (Cit), transcript variant X23, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OMu35781	XM_006530140.3	Mus musculus citron (Cit), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1091.30 $1559.00
OMu35787	XM_006530147.1	Mus musculus citron (Cit), transcript variant X19, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
OMu35789	XM_006530150.3	Mus musculus citron (Cit), transcript variant X22, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$811.30 $1159.00
OMu81234	XM_017320643.1	Mus musculus citron (Cit), transcript variant X25, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OMu81237	XM_017320646.1	Mus musculus citron (Cit), transcript variant X28, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OMu35774	XM_006530130.3	Mus musculus citron (Cit), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1091.30 $1559.00
OMu35775	XM_006530134.3	Mus musculus citron (Cit), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1091.30 $1559.00
OMu35777	XM_006530136.3	Mus musculus citron (Cit), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1091.30 $1559.00
OMu35774	XM_006530131.1	Mus musculus citron (Cit), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1091.30 $1559.00
OMu81235	XM_017320644.1	Mus musculus citron (Cit), transcript variant X26, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OMu35774	XM_006530133.3	Mus musculus citron (Cit), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1091.30 $1559.00
OMu35779	XM_006530138.3	Mus musculus citron (Cit), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1091.30 $1559.00
OMu00646	XM_006530141.2	Mus musculus citron (Cit), transcript variant X12, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1091.30 $1559.00
OMu35778	XM_006530137.3	Mus musculus citron (Cit), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1091.30 $1559.00
OMu00646	XM_006530141.3	Mus musculus citron (Cit), transcript variant X12, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1091.30 $1559.00
OMu35788	XM_006530149.3	Mus musculus citron (Cit), transcript variant X21, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$811.30 $1159.00
OMu35774	XM_006530132.3	Mus musculus citron (Cit), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1091.30 $1559.00
OMu35780	XM_006530139.3	Mus musculus citron (Cit), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1091.30 $1559.00
OMu67373	XM_011248163.2	Mus musculus citron (Cit), transcript variant X17, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1091.30 $1559.00
OMu35790	XM_006530151.3	Mus musculus citron (Cit), transcript variant X30, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$811.30 $1159.00
OMu35791	XM_006530152.1	Mus musculus citron (Cit), transcript variant X31, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$643.30 $919.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Cit ( XM_006530152.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006530215.1 Mus musculus citron (Cit), transcript variant X32, mRNA.

Price & Availability↑

CloneID	OMu35791
Clone ID Related Accession (Same CDS sequence)	XM_006530152.1 , XM_006530152.2
Accession Version	XM_006530152.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3930bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	citron Rho-interacting kinase isoform X27
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000071.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006530152.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGCAGA GACACGAGGA GGAGGCCCAC GAGAAGGGCA AGATCCTCAG CGAGCAGAAG 
GCGATGATCA ACGCGATGGA TTCCAAGATC CGATCCCTGG AGCAGAGGAT CGTGGAGCTG 
TCGGAAGCCA ACAAGCTTGC GGCAAACAGC AGTCTCTTCA CCCAGAGGAA CATGAAGGCC 
CAGGAAGAGA TGATCTCAGA ACTCAGGCAG CAGAAATTTT ACCTGGAGAC GCAGGCCGGA 
AAGCTGGAGG CCCAGAACCG AAAGCTGGAA GAGCAACTGG AGAAGATCAG CCACCAAGAT 
CACAGTGACA AGAGTCGGCT GCTGGAGCTG GAAACAAGGC TGAGGGAGGT CAGCCTGGAG 
CACGAGGAGC AGAAGCTGGA GCTGAAGCGG CAGCTCACGG AGCTGCAGCT GTCCCTGCAG 
GAGCGCGAGT CCCAGCTGAC GGCCCTGCAG GCCGCCCGGG CAGCGCTGGA GAGCCAGCTC 
CGCCAGGCGA AGACAGAGCT GGAGGAGACA ACCGCGGAAG CGGAGGAGGA GATCCAGGCG 
CTCACGGCAC ATCGAGATGA GATCCAGCGC AAATTCGATG CCCTTCGCAA CAGCTGCACG 
GTCATCACCG ACCTGGAGGA GCAGCTGAAC CAGCTCACCG AGGACAACGC CGAGCTCAAC 
AACCAAAACT TCTACCTGTC CAAACAACTC GATGAGGCTT CCGGGGCCAA TGACGAGATT 
GTGCAGCTGC GGAGTGAGGT GGACCATCTC CGCCGTGAGA TCACGGAGCG GGAGATGCAG 
CTCACCAGCC AGAAGCAAGC ACAACTCAGT GCTCCTGATT TGCAGACGAT GGAGGCTCTG 
AAGACGACAT GCACCATGCT GGAGGAGCAG GTCCTGGACC TGGAGGCCCT GAACGACGAG 
CTGCTGGAGA AGGAGCGCCA GTGGGAGGCC TGGCGGAGCG TCCTCGGCGA CGAGAAGTCC 
CAGTTCGAGT GTCGAGTTCG AGAGCTACAG AGGATGCTGG ACACCGAGAA GCAGAGCAGG 
GCTAGGGCCG ATCAGCGGAT CACCGAGTCG CGCCAGGTGG TGGAGTTGGC GGTGAAGGAA 
CACAAGGCCG AGATTCTTGC TCTGCAGCAG GCTCTCAAGG AGCAGAAGCT CAAAGCCGAG 
AGCCTGTCGG ACAAGCTCAA CGACCTGGAG AAGAAGCACG CCATGCTGGA GATGAACGCC 
CGGAGCTTAC AGCAGAAACT AGAGACAGAG CGGGAGCTCA AACAGAGGCT TCTGGAGGAG 
CAAGCCAAAT TGCAGCAGCA GATGGACCTG CAGAAGAACC ACATCTTCAG ACTGACGCAA 
GGGCTGCAGG AGGCGCTGGA CCGGGCGGAT CTGCTGAAGA CAGAAAGGAG CGACCTGGAG 
TACCAGCTGG AAAACATTCA GGTTCTCTAC TCTCACGAGA AAGTGAAAAT GGAAGGCACA 
ATCTCTCAGC AAACCAAACT CATTGATTTC CTGCAAGCCA AAATGGACCA GCCTGCTAAA 
AAGAAAAAGG GTTTATTTAG TCGACGGAAA GAGGACCCTG CTTTGCCCAC ACAGGTTCCT 
CTGCAGTACA ATGAGCTGAA GCTAGCCCTG GAAAAGGAGA AAGCCCGATG CGCGGAGCTG 
GAGGAGGCCC TTCAGAAGAC CCGCATCGAA CTCCGCTCTG CGCGGGAGGA AGCTGCCCAC 
CGCAAAGCCA CGGACCACCC GCACCCGTCT ACGCCAGCCA CTGCGAGGCA GCAGATCGCC 
ATGTCTGCCA TTGTGCGGTC GCCCGAGCAC CAGCCCAGTG CCATGAGCCT GCTTGCCCCA 
CCATCCAGCC GCAGAAAGGA GTCGTCAACT CCAGAGGAAT TCAGCCGGCG TCTGAAAGAG 
CGCATGCACC ACAACATCCC TCACCGGTTT AATGTGGGCC TGAACATGAG AGCCACCAAG 
TGCGCCGTGT GTCTGGATAC TGTGCACTTT GGACGCCAGG CATCCAAATG CCTCGAATGT 
CAAGTGATGT GTCACCCCAA ATGCTCCACC TGCTTGCCCG CTACCTGTGG CCTGCCAGCT 
GAATATGCCA CACACTTCAC TGAGGCCTTC TGCCGGGACA AAATGAACTC CCCGGGGCTC 
CAGAGCAAGG AGCCTGGCAG CAGCTTGCAC CTGGAAGGGT GGATGAAAGT GCCCAGAAAT 
AACAAACGGG GACAGCAAGG CTGGGACAGG AAGTACATTG TTCTGGAGGG GTCAAAAGTC 
CTCATCTATG ACAATGAAGC CAGAGAAGCT GGACAGAGGC CGGTGGAAGA ATTTGAGCTG 
TGCCTTCCTG ACGGGGACGT ATCTATTCAT GGTGCCGTTG GTGCTTCAGA ACTTGCAAAT 
ACCGCCAAAG CAGATGTCCC ATACATCCTG AAGATGGAGT CTCACCCACA CACCACCTGC 
TGGCCTGGGA GAACCCTCTA CTTGCTAGCA CCCAGCTTCC CCGACAAGCA GCGCTGGGTC 
ACCGCCTTAG AATCTGTCGT CGCAGGTGGG AGAGTTTCTA GGGAAAAGGC CGAAGCCGAT 
GCTGCACGTG ACCATACTTC GAGTGAGCAT CAGCCAGTGT GGGTTGAGAA ATTACTTGGA 
AACTCTCTGC TGAAACTGGA GGGCGATGAC CGGCTTGACA TGAACTGCAC CCTGCCCTTC 
AGTGACCAGG TAGTGCTGGT GGGCACGGAG GAAGGCCTCT ACGCCCTGAA TGTCTTGAAA 
AACTCCTTAA CCCACATCCC AGGGATTGGC GCAGTCTTCC AAATTTACAT CATCAAGGAC 
CTGGAGAAGC TGCTCATGAT AGCAGGGGAA GAGCGGGCTC TGTGCCTGGT GGACGTGAAG 
AAGGTGAAGC AGTCCCTGGC GCAGTCACAC CTGCCTGCCC AGCCCGACGT CTCCCCCAAC 
ATATTCGAAG CCGTCAAAGG CTGCCACTTG TTCGCTGCTG GCAAGATCGA GAACAGCCTG 
TGCATCTGCG CCGCTATGCC AAGCAAAGTC GTCATCCTCC GCTACAATGA CAACCTCAGC 
AAGTACTGCA TCCGCAAGGA GATCGAGACC TCAGAGCCCT GCAGCTGTAT CCACTTCACC 
AACTACAGCA TCCTCATTGG GACCAACAAA TTCTATGAGA TCGACATGAA GCAGTACACG 
CTTGACGAGT TCCTGGACAA GAACGACCAT TCCTTGGCAC CTGCTGTGTT CGCCTCCTCG 
TCCAACAGCT TCCCTGTCTC CATTGTGCAG GCGAACAGCG CCGGGCAGCG AGAAGAATAC 
CTGCTGTGCT TCCACGAATT TGGGGTGTTC GTGGATTCTT ACGGAAGACG TAGCCGCACA 
GATGATCTCA AGTGGAGTCG CTTACCTCTG GCCTTCGCCT ACAGAGAACC TTATCTGTTT 
GTGACTCACT TCAACTCCCT GGAAGTCATT GAGATCCAGG CCAGATCCTC ACTGGGGAGC 
CCTGCCCGAG CATATCTGGA AATTCCAAAC CCTCGCTACC TGGGCCCCGC GATTTCCTCC 
GGAGCGATTT ACCTGGCCTC CTCATACCAG GACAAGTTAA GGGTCATATG CTGCAAAGGA 
AACCTCGTGA AGGAGTCAGG CACTGAGCAG CACCGGGTGC CCTCCACCTC CCGCAGCAGC 
CCCAACAAGC GAGGCCCACC AACATACAAC GAGCACATCA CCAAACGCGT GGCCTCCAGC 
CCGGCGCCAC CGGAAGGCCC CAGCCACCCC CGAGAGCCAA GCACACCGCA CCGCTACCGA 
GACAGAGAGG GTCGGACAGA GCTGCGCAGG GACAAGTCTC CAGGCCGCCC TCTGGAGCGG 
GAGAAGTCCC CAGGCCGAAT GCTCAGCACT AGGAGAGAGC GGTCCCCAGG GAGACTGTTT 
GAAGACAGCA GCAGGGGCCG GCTGCCTGCA GGAGCAGTGA GGACCCCACT GTCCCAGGTT 
AACAAGGTCT GGGACCAGTC TTCAGTATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006530215.1
CDS	223..4152
Translation	MMQRHEEEAHEKGKILSEQKAMINAMDSKIRSLEQRIVELSEANKLAANSSLFTQRNMKAQEEMISELRQQKFYLETQAGKLEAQNRKLEEQLEKISHQDHSDKSRLLELETRLREVSLEHEEQKLELKRQLTELQLSLQERESQLTALQAARAALESQLRQAKTELEETTAEAEEEIQALTAHRDEIQRKFDALRNSCTVITDLEEQLNQLTEDNAELNNQNFYLSKQLDEASGANDEIVQLRSEVDHLRREITEREMQLTSQKQAQLSAPDLQTMEALKTTCTMLEEQVLDLEALNDELLEKERQWEAWRSVLGDEKSQFECRVRELQRMLDTEKQSRARADQRITESRQVVELAVKEHKAEILALQQALKEQKLKAESLSDKLNDLEKKHAMLEMNARSLQQKLETERELKQRLLEEQAKLQQQMDLQKNHIFRLTQGLQEALDRADLLKTERSDLEYQLENIQVLYSHEKVKMEGTISQQTKLIDFLQAKMDQPAKKKKGLFSRRKEDPALPTQVPLQYNELKLALEKEKARCAELEEALQKTRIELRSAREEAAHRKATDHPHPSTPATARQQIAMSAIVRSPEHQPSAMSLLAPPSSRRKESSTPEEFSRRLKERMHHNIPHRFNVGLNMRATKCAVCLDTVHFGRQASKCLECQVMCHPKCSTCLPATCGLPAEYATHFTEAFCRDKMNSPGLQSKEPGSSLHLEGWMKVPRNNKRGQQGWDRKYIVLEGSKVLIYDNEAREAGQRPVEEFELCLPDGDVSIHGAVGASELANTAKADVPYILKMESHPHTTCWPGRTLYLLAPSFPDKQRWVTALESVVAGGRVSREKAEADAARDHTSSEHQPVWVEKLLGNSLLKLEGDDRLDMNCTLPFSDQVVLVGTEEGLYALNVLKNSLTHIPGIGAVFQIYIIKDLEKLLMIAGEERALCLVDVKKVKQSLAQSHLPAQPDVSPNIFEAVKGCHLFAAGKIENSLCICAAMPSKVVILRYNDNLSKYCIRKEIETSEPCSCIHFTNYSILIGTNKFYEIDMKQYTLDEFLDKNDHSLAPAVFASSSNSFPVSIVQANSAGQREEYLLCFHEFGVFVDSYGRRSRTDDLKWSRLPLAFAYREPYLFVTHFNSLEVIEIQARSSLGSPARAYLEIPNPRYLGPAISSGAIYLASSYQDKLRVICCKGNLVKESGTEQHRVPSTSRSSPNKRGPPTYNEHITKRVASSPAPPEGPSHPREPSTPHRYRDREGRTELRRDKSPGRPLEREKSPGRMLSTRRERSPGRLFEDSSRGRLPAGAVRTPLSQVNKVWDQSSV

Target ORF information:

RefSeq Version	XM_006530152.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus citron (Cit), transcript variant X32, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006530152.2

ORF Insert Sequence:

ATGATGCAGA GACACGAGGA GGAGGCCCAC GAGAAGGGCA AGATCCTCAG CGAGCAGAAG 
GCGATGATCA ACGCGATGGA TTCCAAGATC CGATCCCTGG AGCAGAGGAT CGTGGAGCTG 
TCGGAAGCCA ACAAGCTTGC GGCAAACAGC AGTCTCTTCA CCCAGAGGAA CATGAAGGCC 
CAGGAAGAGA TGATCTCAGA ACTCAGGCAG CAGAAATTTT ACCTGGAGAC GCAGGCCGGA 
AAGCTGGAGG CCCAGAACCG AAAGCTGGAA GAGCAACTGG AGAAGATCAG CCACCAAGAT 
CACAGTGACA AGAGTCGGCT GCTGGAGCTG GAAACAAGGC TGAGGGAGGT CAGCCTGGAG 
CACGAGGAGC AGAAGCTGGA GCTGAAGCGG CAGCTCACGG AGCTGCAGCT GTCCCTGCAG 
GAGCGCGAGT CCCAGCTGAC GGCCCTGCAG GCCGCCCGGG CAGCGCTGGA GAGCCAGCTC 
CGCCAGGCGA AGACAGAGCT GGAGGAGACA ACCGCGGAAG CGGAGGAGGA GATCCAGGCG 
CTCACGGCAC ATCGAGATGA GATCCAGCGC AAATTCGATG CCCTTCGCAA CAGCTGCACG 
GTCATCACCG ACCTGGAGGA GCAGCTGAAC CAGCTCACCG AGGACAACGC CGAGCTCAAC 
AACCAAAACT TCTACCTGTC CAAACAACTC GATGAGGCTT CCGGGGCCAA TGACGAGATT 
GTGCAGCTGC GGAGTGAGGT GGACCATCTC CGCCGTGAGA TCACGGAGCG GGAGATGCAG 
CTCACCAGCC AGAAGCAAGC ACAACTCAGT GCTCCTGATT TGCAGACGAT GGAGGCTCTG 
AAGACGACAT GCACCATGCT GGAGGAGCAG GTCCTGGACC TGGAGGCCCT GAACGACGAG 
CTGCTGGAGA AGGAGCGCCA GTGGGAGGCC TGGCGGAGCG TCCTCGGCGA CGAGAAGTCC 
CAGTTCGAGT GTCGAGTTCG AGAGCTACAG AGGATGCTGG ACACCGAGAA GCAGAGCAGG 
GCTAGGGCCG ATCAGCGGAT CACCGAGTCG CGCCAGGTGG TGGAGTTGGC GGTGAAGGAA 
CACAAGGCCG AGATTCTTGC TCTGCAGCAG GCTCTCAAGG AGCAGAAGCT CAAAGCCGAG 
AGCCTGTCGG ACAAGCTCAA CGACCTGGAG AAGAAGCACG CCATGCTGGA GATGAACGCC 
CGGAGCTTAC AGCAGAAACT AGAGACAGAG CGGGAGCTCA AACAGAGGCT TCTGGAGGAG 
CAAGCCAAAT TGCAGCAGCA GATGGACCTG CAGAAGAACC ACATCTTCAG ACTGACGCAA 
GGGCTGCAGG AGGCGCTGGA CCGGGCGGAT CTGCTGAAGA CAGAAAGGAG CGACCTGGAG 
TACCAGCTGG AAAACATTCA GGTTCTCTAC TCTCACGAGA AAGTGAAAAT GGAAGGCACA 
ATCTCTCAGC AAACCAAACT CATTGATTTC CTGCAAGCCA AAATGGACCA GCCTGCTAAA 
AAGAAAAAGG GTTTATTTAG TCGACGGAAA GAGGACCCTG CTTTGCCCAC ACAGGTTCCT 
CTGCAGTACA ATGAGCTGAA GCTAGCCCTG GAAAAGGAGA AAGCCCGATG CGCGGAGCTG 
GAGGAGGCCC TTCAGAAGAC CCGCATCGAA CTCCGCTCTG CGCGGGAGGA AGCTGCCCAC 
CGCAAAGCCA CGGACCACCC GCACCCGTCT ACGCCAGCCA CTGCGAGGCA GCAGATCGCC 
ATGTCTGCCA TTGTGCGGTC GCCCGAGCAC CAGCCCAGTG CCATGAGCCT GCTTGCCCCA 
CCATCCAGCC GCAGAAAGGA GTCGTCAACT CCAGAGGAAT TCAGCCGGCG TCTGAAAGAG 
CGCATGCACC ACAACATCCC TCACCGGTTT AATGTGGGCC TGAACATGAG AGCCACCAAG 
TGCGCCGTGT GTCTGGATAC TGTGCACTTT GGACGCCAGG CATCCAAATG CCTCGAATGT 
CAAGTGATGT GTCACCCCAA ATGCTCCACC TGCTTGCCCG CTACCTGTGG CCTGCCAGCT 
GAATATGCCA CACACTTCAC TGAGGCCTTC TGCCGGGACA AAATGAACTC CCCGGGGCTC 
CAGAGCAAGG AGCCTGGCAG CAGCTTGCAC CTGGAAGGGT GGATGAAAGT GCCCAGAAAT 
AACAAACGGG GACAGCAAGG CTGGGACAGG AAGTACATTG TTCTGGAGGG GTCAAAAGTC 
CTCATCTATG ACAATGAAGC CAGAGAAGCT GGACAGAGGC CGGTGGAAGA ATTTGAGCTG 
TGCCTTCCTG ACGGGGACGT ATCTATTCAT GGTGCCGTTG GTGCTTCAGA ACTTGCAAAT 
ACCGCCAAAG CAGATGTCCC ATACATCCTG AAGATGGAGT CTCACCCACA CACCACCTGC 
TGGCCTGGGA GAACCCTCTA CTTGCTAGCA CCCAGCTTCC CCGACAAGCA GCGCTGGGTC 
ACCGCCTTAG AATCTGTCGT CGCAGGTGGG AGAGTTTCTA GGGAAAAGGC CGAAGCCGAT 
GCTGCACGTG ACCATACTTC GAGTGAGCAT CAGCCAGTGT GGGTTGAGAA ATTACTTGGA 
AACTCTCTGC TGAAACTGGA GGGCGATGAC CGGCTTGACA TGAACTGCAC CCTGCCCTTC 
AGTGACCAGG TAGTGCTGGT GGGCACGGAG GAAGGCCTCT ACGCCCTGAA TGTCTTGAAA 
AACTCCTTAA CCCACATCCC AGGGATTGGC GCAGTCTTCC AAATTTACAT CATCAAGGAC 
CTGGAGAAGC TGCTCATGAT AGCAGGGGAA GAGCGGGCTC TGTGCCTGGT GGACGTGAAG 
AAGGTGAAGC AGTCCCTGGC GCAGTCACAC CTGCCTGCCC AGCCCGACGT CTCCCCCAAC 
ATATTCGAAG CCGTCAAAGG CTGCCACTTG TTCGCTGCTG GCAAGATCGA GAACAGCCTG 
TGCATCTGCG CCGCTATGCC AAGCAAAGTC GTCATCCTCC GCTACAATGA CAACCTCAGC 
AAGTACTGCA TCCGCAAGGA GATCGAGACC TCAGAGCCCT GCAGCTGTAT CCACTTCACC 
AACTACAGCA TCCTCATTGG GACCAACAAA TTCTATGAGA TCGACATGAA GCAGTACACG 
CTTGACGAGT TCCTGGACAA GAACGACCAT TCCTTGGCAC CTGCTGTGTT CGCCTCCTCG 
TCCAACAGCT TCCCTGTCTC CATTGTGCAG GCGAACAGCG CCGGGCAGCG AGAAGAATAC 
CTGCTGTGCT TCCACGAATT TGGGGTGTTC GTGGATTCTT ACGGAAGACG TAGCCGCACA 
GATGATCTCA AGTGGAGTCG CTTACCTCTG GCCTTCGCCT ACAGAGAACC TTATCTGTTT 
GTGACTCACT TCAACTCCCT GGAAGTCATT GAGATCCAGG CCAGATCCTC ACTGGGGAGC 
CCTGCCCGAG CATATCTGGA AATTCCAAAC CCTCGCTACC TGGGCCCCGC GATTTCCTCC 
GGAGCGATTT ACCTGGCCTC CTCATACCAG GACAAGTTAA GGGTCATATG CTGCAAAGGA 
AACCTCGTGA AGGAGTCAGG CACTGAGCAG CACCGGGTGC CCTCCACCTC CCGCAGCAGC 
CCCAACAAGC GAGGCCCACC AACATACAAC GAGCACATCA CCAAACGCGT GGCCTCCAGC 
CCGGCGCCAC CGGAAGGCCC CAGCCACCCC CGAGAGCCAA GCACACCGCA CCGCTACCGA 
GACAGAGAGG GTCGGACAGA GCTGCGCAGG GACAAGTCTC CAGGCCGCCC TCTGGAGCGG 
GAGAAGTCCC CAGGCCGAAT GCTCAGCACT AGGAGAGAGC GGTCCCCAGG GAGACTGTTT 
GAAGACAGCA GCAGGGGCCG GCTGCCTGCA GGAGCAGTGA GGACCCCACT GTCCCAGGTT 
AACAAGGTCT GGGACCAGTC TTCAGTATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cit ( XM_006530152.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006530215.1 Mus musculus citron (Cit), transcript variant X31, mRNA.

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CloneID	OMu35791
Clone ID Related Accession (Same CDS sequence)	XM_006530152.1 , XM_006530152.2
Accession Version	XM_006530152.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3930bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	citron Rho-interacting kinase isoform X27
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000071.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGCAGA GACACGAGGA GGAGGCCCAC GAGAAGGGCA AGATCCTCAG CGAGCAGAAG 
GCGATGATCA ACGCGATGGA TTCCAAGATC CGATCCCTGG AGCAGAGGAT CGTGGAGCTG 
TCGGAAGCCA ACAAGCTTGC GGCAAACAGC AGTCTCTTCA CCCAGAGGAA CATGAAGGCC 
CAGGAAGAGA TGATCTCAGA ACTCAGGCAG CAGAAATTTT ACCTGGAGAC GCAGGCCGGA 
AAGCTGGAGG CCCAGAACCG AAAGCTGGAA GAGCAACTGG AGAAGATCAG CCACCAAGAT 
CACAGTGACA AGAGTCGGCT GCTGGAGCTG GAAACAAGGC TGAGGGAGGT CAGCCTGGAG 
CACGAGGAGC AGAAGCTGGA GCTGAAGCGG CAGCTCACGG AGCTGCAGCT GTCCCTGCAG 
GAGCGCGAGT CCCAGCTGAC GGCCCTGCAG GCCGCCCGGG CAGCGCTGGA GAGCCAGCTC 
CGCCAGGCGA AGACAGAGCT GGAGGAGACA ACCGCGGAAG CGGAGGAGGA GATCCAGGCG 
CTCACGGCAC ATCGAGATGA GATCCAGCGC AAATTCGATG CCCTTCGCAA CAGCTGCACG 
GTCATCACCG ACCTGGAGGA GCAGCTGAAC CAGCTCACCG AGGACAACGC CGAGCTCAAC 
AACCAAAACT TCTACCTGTC CAAACAACTC GATGAGGCTT CCGGGGCCAA TGACGAGATT 
GTGCAGCTGC GGAGTGAGGT GGACCATCTC CGCCGTGAGA TCACGGAGCG GGAGATGCAG 
CTCACCAGCC AGAAGCAAGC ACAACTCAGT GCTCCTGATT TGCAGACGAT GGAGGCTCTG 
AAGACGACAT GCACCATGCT GGAGGAGCAG GTCCTGGACC TGGAGGCCCT GAACGACGAG 
CTGCTGGAGA AGGAGCGCCA GTGGGAGGCC TGGCGGAGCG TCCTCGGCGA CGAGAAGTCC 
CAGTTCGAGT GTCGAGTTCG AGAGCTACAG AGGATGCTGG ACACCGAGAA GCAGAGCAGG 
GCTAGGGCCG ATCAGCGGAT CACCGAGTCG CGCCAGGTGG TGGAGTTGGC GGTGAAGGAA 
CACAAGGCCG AGATTCTTGC TCTGCAGCAG GCTCTCAAGG AGCAGAAGCT CAAAGCCGAG 
AGCCTGTCGG ACAAGCTCAA CGACCTGGAG AAGAAGCACG CCATGCTGGA GATGAACGCC 
CGGAGCTTAC AGCAGAAACT AGAGACAGAG CGGGAGCTCA AACAGAGGCT TCTGGAGGAG 
CAAGCCAAAT TGCAGCAGCA GATGGACCTG CAGAAGAACC ACATCTTCAG ACTGACGCAA 
GGGCTGCAGG AGGCGCTGGA CCGGGCGGAT CTGCTGAAGA CAGAAAGGAG CGACCTGGAG 
TACCAGCTGG AAAACATTCA GGTTCTCTAC TCTCACGAGA AAGTGAAAAT GGAAGGCACA 
ATCTCTCAGC AAACCAAACT CATTGATTTC CTGCAAGCCA AAATGGACCA GCCTGCTAAA 
AAGAAAAAGG GTTTATTTAG TCGACGGAAA GAGGACCCTG CTTTGCCCAC ACAGGTTCCT 
CTGCAGTACA ATGAGCTGAA GCTAGCCCTG GAAAAGGAGA AAGCCCGATG CGCGGAGCTG 
GAGGAGGCCC TTCAGAAGAC CCGCATCGAA CTCCGCTCTG CGCGGGAGGA AGCTGCCCAC 
CGCAAAGCCA CGGACCACCC GCACCCGTCT ACGCCAGCCA CTGCGAGGCA GCAGATCGCC 
ATGTCTGCCA TTGTGCGGTC GCCCGAGCAC CAGCCCAGTG CCATGAGCCT GCTTGCCCCA 
CCATCCAGCC GCAGAAAGGA GTCGTCAACT CCAGAGGAAT TCAGCCGGCG TCTGAAAGAG 
CGCATGCACC ACAACATCCC TCACCGGTTT AATGTGGGCC TGAACATGAG AGCCACCAAG 
TGCGCCGTGT GTCTGGATAC TGTGCACTTT GGACGCCAGG CATCCAAATG CCTCGAATGT 
CAAGTGATGT GTCACCCCAA ATGCTCCACC TGCTTGCCCG CTACCTGTGG CCTGCCAGCT 
GAATATGCCA CACACTTCAC TGAGGCCTTC TGCCGGGACA AAATGAACTC CCCGGGGCTC 
CAGAGCAAGG AGCCTGGCAG CAGCTTGCAC CTGGAAGGGT GGATGAAAGT GCCCAGAAAT 
AACAAACGGG GACAGCAAGG CTGGGACAGG AAGTACATTG TTCTGGAGGG GTCAAAAGTC 
CTCATCTATG ACAATGAAGC CAGAGAAGCT GGACAGAGGC CGGTGGAAGA ATTTGAGCTG 
TGCCTTCCTG ACGGGGACGT ATCTATTCAT GGTGCCGTTG GTGCTTCAGA ACTTGCAAAT 
ACCGCCAAAG CAGATGTCCC ATACATCCTG AAGATGGAGT CTCACCCACA CACCACCTGC 
TGGCCTGGGA GAACCCTCTA CTTGCTAGCA CCCAGCTTCC CCGACAAGCA GCGCTGGGTC 
ACCGCCTTAG AATCTGTCGT CGCAGGTGGG AGAGTTTCTA GGGAAAAGGC CGAAGCCGAT 
GCTGCACGTG ACCATACTTC GAGTGAGCAT CAGCCAGTGT GGGTTGAGAA ATTACTTGGA 
AACTCTCTGC TGAAACTGGA GGGCGATGAC CGGCTTGACA TGAACTGCAC CCTGCCCTTC 
AGTGACCAGG TAGTGCTGGT GGGCACGGAG GAAGGCCTCT ACGCCCTGAA TGTCTTGAAA 
AACTCCTTAA CCCACATCCC AGGGATTGGC GCAGTCTTCC AAATTTACAT CATCAAGGAC 
CTGGAGAAGC TGCTCATGAT AGCAGGGGAA GAGCGGGCTC TGTGCCTGGT GGACGTGAAG 
AAGGTGAAGC AGTCCCTGGC GCAGTCACAC CTGCCTGCCC AGCCCGACGT CTCCCCCAAC 
ATATTCGAAG CCGTCAAAGG CTGCCACTTG TTCGCTGCTG GCAAGATCGA GAACAGCCTG 
TGCATCTGCG CCGCTATGCC AAGCAAAGTC GTCATCCTCC GCTACAATGA CAACCTCAGC 
AAGTACTGCA TCCGCAAGGA GATCGAGACC TCAGAGCCCT GCAGCTGTAT CCACTTCACC 
AACTACAGCA TCCTCATTGG GACCAACAAA TTCTATGAGA TCGACATGAA GCAGTACACG 
CTTGACGAGT TCCTGGACAA GAACGACCAT TCCTTGGCAC CTGCTGTGTT CGCCTCCTCG 
TCCAACAGCT TCCCTGTCTC CATTGTGCAG GCGAACAGCG CCGGGCAGCG AGAAGAATAC 
CTGCTGTGCT TCCACGAATT TGGGGTGTTC GTGGATTCTT ACGGAAGACG TAGCCGCACA 
GATGATCTCA AGTGGAGTCG CTTACCTCTG GCCTTCGCCT ACAGAGAACC TTATCTGTTT 
GTGACTCACT TCAACTCCCT GGAAGTCATT GAGATCCAGG CCAGATCCTC ACTGGGGAGC 
CCTGCCCGAG CATATCTGGA AATTCCAAAC CCTCGCTACC TGGGCCCCGC GATTTCCTCC 
GGAGCGATTT ACCTGGCCTC CTCATACCAG GACAAGTTAA GGGTCATATG CTGCAAAGGA 
AACCTCGTGA AGGAGTCAGG CACTGAGCAG CACCGGGTGC CCTCCACCTC CCGCAGCAGC 
CCCAACAAGC GAGGCCCACC AACATACAAC GAGCACATCA CCAAACGCGT GGCCTCCAGC 
CCGGCGCCAC CGGAAGGCCC CAGCCACCCC CGAGAGCCAA GCACACCGCA CCGCTACCGA 
GACAGAGAGG GTCGGACAGA GCTGCGCAGG GACAAGTCTC CAGGCCGCCC TCTGGAGCGG 
GAGAAGTCCC CAGGCCGAAT GCTCAGCACT AGGAGAGAGC GGTCCCCAGG GAGACTGTTT 
GAAGACAGCA GCAGGGGCCG GCTGCCTGCA GGAGCAGTGA GGACCCCACT GTCCCAGGTT 
AACAAGGTCT GGGACCAGTC TTCAGTATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006530215.1
CDS	223..4152
Translation	MMQRHEEEAHEKGKILSEQKAMINAMDSKIRSLEQRIVELSEANKLAANSSLFTQRNMKAQEEMISELRQQKFYLETQAGKLEAQNRKLEEQLEKISHQDHSDKSRLLELETRLREVSLEHEEQKLELKRQLTELQLSLQERESQLTALQAARAALESQLRQAKTELEETTAEAEEEIQALTAHRDEIQRKFDALRNSCTVITDLEEQLNQLTEDNAELNNQNFYLSKQLDEASGANDEIVQLRSEVDHLRREITEREMQLTSQKQAQLSAPDLQTMEALKTTCTMLEEQVLDLEALNDELLEKERQWEAWRSVLGDEKSQFECRVRELQRMLDTEKQSRARADQRITESRQVVELAVKEHKAEILALQQALKEQKLKAESLSDKLNDLEKKHAMLEMNARSLQQKLETERELKQRLLEEQAKLQQQMDLQKNHIFRLTQGLQEALDRADLLKTERSDLEYQLENIQVLYSHEKVKMEGTISQQTKLIDFLQAKMDQPAKKKKGLFSRRKEDPALPTQVPLQYNELKLALEKEKARCAELEEALQKTRIELRSAREEAAHRKATDHPHPSTPATARQQIAMSAIVRSPEHQPSAMSLLAPPSSRRKESSTPEEFSRRLKERMHHNIPHRFNVGLNMRATKCAVCLDTVHFGRQASKCLECQVMCHPKCSTCLPATCGLPAEYATHFTEAFCRDKMNSPGLQSKEPGSSLHLEGWMKVPRNNKRGQQGWDRKYIVLEGSKVLIYDNEAREAGQRPVEEFELCLPDGDVSIHGAVGASELANTAKADVPYILKMESHPHTTCWPGRTLYLLAPSFPDKQRWVTALESVVAGGRVSREKAEADAARDHTSSEHQPVWVEKLLGNSLLKLEGDDRLDMNCTLPFSDQVVLVGTEEGLYALNVLKNSLTHIPGIGAVFQIYIIKDLEKLLMIAGEERALCLVDVKKVKQSLAQSHLPAQPDVSPNIFEAVKGCHLFAAGKIENSLCICAAMPSKVVILRYNDNLSKYCIRKEIETSEPCSCIHFTNYSILIGTNKFYEIDMKQYTLDEFLDKNDHSLAPAVFASSSNSFPVSIVQANSAGQREEYLLCFHEFGVFVDSYGRRSRTDDLKWSRLPLAFAYREPYLFVTHFNSLEVIEIQARSSLGSPARAYLEIPNPRYLGPAISSGAIYLASSYQDKLRVICCKGNLVKESGTEQHRVPSTSRSSPNKRGPPTYNEHITKRVASSPAPPEGPSHPREPSTPHRYRDREGRTELRRDKSPGRPLEREKSPGRMLSTRRERSPGRLFEDSSRGRLPAGAVRTPLSQVNKVWDQSSV

Target ORF information:

RefSeq Version	XM_006530152.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus citron (Cit), transcript variant X31, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006530152.1

ORF Insert Sequence:

ATGATGCAGA GACACGAGGA GGAGGCCCAC GAGAAGGGCA AGATCCTCAG CGAGCAGAAG 
GCGATGATCA ACGCGATGGA TTCCAAGATC CGATCCCTGG AGCAGAGGAT CGTGGAGCTG 
TCGGAAGCCA ACAAGCTTGC GGCAAACAGC AGTCTCTTCA CCCAGAGGAA CATGAAGGCC 
CAGGAAGAGA TGATCTCAGA ACTCAGGCAG CAGAAATTTT ACCTGGAGAC GCAGGCCGGA 
AAGCTGGAGG CCCAGAACCG AAAGCTGGAA GAGCAACTGG AGAAGATCAG CCACCAAGAT 
CACAGTGACA AGAGTCGGCT GCTGGAGCTG GAAACAAGGC TGAGGGAGGT CAGCCTGGAG 
CACGAGGAGC AGAAGCTGGA GCTGAAGCGG CAGCTCACGG AGCTGCAGCT GTCCCTGCAG 
GAGCGCGAGT CCCAGCTGAC GGCCCTGCAG GCCGCCCGGG CAGCGCTGGA GAGCCAGCTC 
CGCCAGGCGA AGACAGAGCT GGAGGAGACA ACCGCGGAAG CGGAGGAGGA GATCCAGGCG 
CTCACGGCAC ATCGAGATGA GATCCAGCGC AAATTCGATG CCCTTCGCAA CAGCTGCACG 
GTCATCACCG ACCTGGAGGA GCAGCTGAAC CAGCTCACCG AGGACAACGC CGAGCTCAAC 
AACCAAAACT TCTACCTGTC CAAACAACTC GATGAGGCTT CCGGGGCCAA TGACGAGATT 
GTGCAGCTGC GGAGTGAGGT GGACCATCTC CGCCGTGAGA TCACGGAGCG GGAGATGCAG 
CTCACCAGCC AGAAGCAAGC ACAACTCAGT GCTCCTGATT TGCAGACGAT GGAGGCTCTG 
AAGACGACAT GCACCATGCT GGAGGAGCAG GTCCTGGACC TGGAGGCCCT GAACGACGAG 
CTGCTGGAGA AGGAGCGCCA GTGGGAGGCC TGGCGGAGCG TCCTCGGCGA CGAGAAGTCC 
CAGTTCGAGT GTCGAGTTCG AGAGCTACAG AGGATGCTGG ACACCGAGAA GCAGAGCAGG 
GCTAGGGCCG ATCAGCGGAT CACCGAGTCG CGCCAGGTGG TGGAGTTGGC GGTGAAGGAA 
CACAAGGCCG AGATTCTTGC TCTGCAGCAG GCTCTCAAGG AGCAGAAGCT CAAAGCCGAG 
AGCCTGTCGG ACAAGCTCAA CGACCTGGAG AAGAAGCACG CCATGCTGGA GATGAACGCC 
CGGAGCTTAC AGCAGAAACT AGAGACAGAG CGGGAGCTCA AACAGAGGCT TCTGGAGGAG 
CAAGCCAAAT TGCAGCAGCA GATGGACCTG CAGAAGAACC ACATCTTCAG ACTGACGCAA 
GGGCTGCAGG AGGCGCTGGA CCGGGCGGAT CTGCTGAAGA CAGAAAGGAG CGACCTGGAG 
TACCAGCTGG AAAACATTCA GGTTCTCTAC TCTCACGAGA AAGTGAAAAT GGAAGGCACA 
ATCTCTCAGC AAACCAAACT CATTGATTTC CTGCAAGCCA AAATGGACCA GCCTGCTAAA 
AAGAAAAAGG GTTTATTTAG TCGACGGAAA GAGGACCCTG CTTTGCCCAC ACAGGTTCCT 
CTGCAGTACA ATGAGCTGAA GCTAGCCCTG GAAAAGGAGA AAGCCCGATG CGCGGAGCTG 
GAGGAGGCCC TTCAGAAGAC CCGCATCGAA CTCCGCTCTG CGCGGGAGGA AGCTGCCCAC 
CGCAAAGCCA CGGACCACCC GCACCCGTCT ACGCCAGCCA CTGCGAGGCA GCAGATCGCC 
ATGTCTGCCA TTGTGCGGTC GCCCGAGCAC CAGCCCAGTG CCATGAGCCT GCTTGCCCCA 
CCATCCAGCC GCAGAAAGGA GTCGTCAACT CCAGAGGAAT TCAGCCGGCG TCTGAAAGAG 
CGCATGCACC ACAACATCCC TCACCGGTTT AATGTGGGCC TGAACATGAG AGCCACCAAG 
TGCGCCGTGT GTCTGGATAC TGTGCACTTT GGACGCCAGG CATCCAAATG CCTCGAATGT 
CAAGTGATGT GTCACCCCAA ATGCTCCACC TGCTTGCCCG CTACCTGTGG CCTGCCAGCT 
GAATATGCCA CACACTTCAC TGAGGCCTTC TGCCGGGACA AAATGAACTC CCCGGGGCTC 
CAGAGCAAGG AGCCTGGCAG CAGCTTGCAC CTGGAAGGGT GGATGAAAGT GCCCAGAAAT 
AACAAACGGG GACAGCAAGG CTGGGACAGG AAGTACATTG TTCTGGAGGG GTCAAAAGTC 
CTCATCTATG ACAATGAAGC CAGAGAAGCT GGACAGAGGC CGGTGGAAGA ATTTGAGCTG 
TGCCTTCCTG ACGGGGACGT ATCTATTCAT GGTGCCGTTG GTGCTTCAGA ACTTGCAAAT 
ACCGCCAAAG CAGATGTCCC ATACATCCTG AAGATGGAGT CTCACCCACA CACCACCTGC 
TGGCCTGGGA GAACCCTCTA CTTGCTAGCA CCCAGCTTCC CCGACAAGCA GCGCTGGGTC 
ACCGCCTTAG AATCTGTCGT CGCAGGTGGG AGAGTTTCTA GGGAAAAGGC CGAAGCCGAT 
GCTGCACGTG ACCATACTTC GAGTGAGCAT CAGCCAGTGT GGGTTGAGAA ATTACTTGGA 
AACTCTCTGC TGAAACTGGA GGGCGATGAC CGGCTTGACA TGAACTGCAC CCTGCCCTTC 
AGTGACCAGG TAGTGCTGGT GGGCACGGAG GAAGGCCTCT ACGCCCTGAA TGTCTTGAAA 
AACTCCTTAA CCCACATCCC AGGGATTGGC GCAGTCTTCC AAATTTACAT CATCAAGGAC 
CTGGAGAAGC TGCTCATGAT AGCAGGGGAA GAGCGGGCTC TGTGCCTGGT GGACGTGAAG 
AAGGTGAAGC AGTCCCTGGC GCAGTCACAC CTGCCTGCCC AGCCCGACGT CTCCCCCAAC 
ATATTCGAAG CCGTCAAAGG CTGCCACTTG TTCGCTGCTG GCAAGATCGA GAACAGCCTG 
TGCATCTGCG CCGCTATGCC AAGCAAAGTC GTCATCCTCC GCTACAATGA CAACCTCAGC 
AAGTACTGCA TCCGCAAGGA GATCGAGACC TCAGAGCCCT GCAGCTGTAT CCACTTCACC 
AACTACAGCA TCCTCATTGG GACCAACAAA TTCTATGAGA TCGACATGAA GCAGTACACG 
CTTGACGAGT TCCTGGACAA GAACGACCAT TCCTTGGCAC CTGCTGTGTT CGCCTCCTCG 
TCCAACAGCT TCCCTGTCTC CATTGTGCAG GCGAACAGCG CCGGGCAGCG AGAAGAATAC 
CTGCTGTGCT TCCACGAATT TGGGGTGTTC GTGGATTCTT ACGGAAGACG TAGCCGCACA 
GATGATCTCA AGTGGAGTCG CTTACCTCTG GCCTTCGCCT ACAGAGAACC TTATCTGTTT 
GTGACTCACT TCAACTCCCT GGAAGTCATT GAGATCCAGG CCAGATCCTC ACTGGGGAGC 
CCTGCCCGAG CATATCTGGA AATTCCAAAC CCTCGCTACC TGGGCCCCGC GATTTCCTCC 
GGAGCGATTT ACCTGGCCTC CTCATACCAG GACAAGTTAA GGGTCATATG CTGCAAAGGA 
AACCTCGTGA AGGAGTCAGG CACTGAGCAG CACCGGGTGC CCTCCACCTC CCGCAGCAGC 
CCCAACAAGC GAGGCCCACC AACATACAAC GAGCACATCA CCAAACGCGT GGCCTCCAGC 
CCGGCGCCAC CGGAAGGCCC CAGCCACCCC CGAGAGCCAA GCACACCGCA CCGCTACCGA 
GACAGAGAGG GTCGGACAGA GCTGCGCAGG GACAAGTCTC CAGGCCGCCC TCTGGAGCGG 
GAGAAGTCCC CAGGCCGAAT GCTCAGCACT AGGAGAGAGC GGTCCCCAGG GAGACTGTTT 
GAAGACAGCA GCAGGGGCCG GCTGCCTGCA GGAGCAGTGA GGACCCCACT GTCCCAGGTT 
AACAAGGTCT GGGACCAGTC TTCAGTATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cit ( XM_006530153.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006530216.1 Mus musculus citron (Cit), transcript variant X33, mRNA.

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CloneID	OMu35792
Clone ID Related Accession (Same CDS sequence)	XM_006530153.1
Accession Version	XM_006530153.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3531bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	citron Rho-interacting kinase isoform X28
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000071.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGCAGA GACACGAGGA GGAGGCCCAC GAGAAGGGCA AGATCCTCAG CGAGCAGAAG 
GCGATGATCA ACGCGATGGA TTCCAAGATC CGATCCCTGG AGCAGAGGAT CGTGGAGCTG 
TCGGAAGCCA ACAAGCTTGC GGCAAACAGC AGTCTCTTCA CCCAGAGGAA CATGAAGGCC 
CAGGAAGAGA TGATCTCAGA ACTCAGGCAG CAGAAATTTT ACCTGGAGAC GCAGGCCGGA 
AAGCTGGAGG CCCAGAACCG AAAGCTGGAA GAGCAACTGG AGAAGATCAG CCACCAAGAT 
CACAGTGACA AGAGTCGGCT GCTGGAGCTG GAAACAAGGC TGAGGGAGGT CAGCCTGGAG 
CACGAGGAGC AGAAGCTGGA GCTGAAGCGG CAGCTCACGG AGCTGCAGCT GTCCCTGCAG 
GAGCGCGAGT CCCAGCTGAC GGCCCTGCAG GCCGCCCGGG CAGCGCTGGA GAGCCAGCTC 
CGCCAGGCGA AGACAGAGCT GGAGGAGACA ACCGCGGAAG CGGAGGAGGA GATCCAGGCG 
CTCACGGCAC ATCGAGATGA GATCCAGCGC AAATTCGATG CCCTTCGCAA CAGCTGCACG 
GTCATCACCG ACCTGGAGGA GCAGCTGAAC CAGCTCACCG AGGACAACGC CGAGCTCAAC 
AACCAAAACT TCTACCTGTC CAAACAACTC GATGAGGCTT CCGGGGCCAA TGACGAGATT 
GTGCAGCTGC GGAGTGAGGT GGACCATCTC CGCCGTGAGA TCACGGAGCG GGAGATGCAG 
CTCACCAGCC AGAAGCAAAC GATGGAGGCT CTGAAGACGA CATGCACCAT GCTGGAGGAG 
CAGGTCCTGG ACCTGGAGGC CCTGAACGAC GAGCTGCTGG AGAAGGAGCG CCAGTGGGAG 
GCCTGGCGGA GCGTCCTCGG CGACGAGAAG TCCCAGTTCG AGTGTCGAGT TCGAGAGCTA 
CAGAGGATGC TGGACACCGA GAAGCAGAGC AGGGCTAGGG CCGATCAGCG GATCACCGAG 
TCGCGCCAGG TGGTGGAGTT GGCGGTGAAG GAACACAAGG CCGAGATTCT TGCTCTGCAG 
CAGGCTCTCA AGGAGCAGAA GCTCAAAGCC GAGAGCCTGT CGGACAAGCT CAACGACCTG 
GAGAAGAAGC ACGCCATGCT GGAGATGAAC GCCCGGAGCT TACAGCAGAA ACTAGAGACA 
GAGCGGGAGC TCAAACAGAG GCTTCTGGAG GAGCAAGCCA AATTGCAGCA GCAGATGGAC 
CTGCAGAAGA ACCACATCTT CAGACTGACG CAAGGGCTGC AGGAGGCGCT GGACCGGGCG 
GATCTGCTGA AGACAGAAAG GAGCGACCTG GAGTACCAGC TGGAAAACAT TCAGGTTCTC 
TACTCTCACG AGAAAGTGAA AATGGAAGGC ACAATCTCTC AGCAAACCAA ACTCATTGAT 
TTCCTGCAAG CCAAAATGGA CCAGCCTGCT AAAAAGAAAA AGGGTTTATT TAGTCGACGG 
AAAGAGGACC CTGCTTTGCC CACACAGGTT CCTCTGCAGT ACAATGAGCT GAAGCTAGCC 
CTGGAAAAGG AGAAAGCCCG ATGCGCGGAG CTGGAGGAGG CCCTTCAGAA GACCCGCATC 
GAACTCCGCT CTGCGCGGGA GGAAGCTGCC CACCGCAAAG CCACGGACCA CCCGCACCCG 
TCTACGCCAG CCACTGCGAG GCAGCAGATC GCCATGTCTG CCATTGTGCG GTCGCCCGAG 
CACCAGCCCA GTGCCATGAG CCTGCTTGCC CCACCATCCA GCCGCAGAAA GGAGTCGTCA 
ACTCCAGAGG AATTCAGCCG GCGTCTGAAA GAGCGCATGC ACCACAACAT CCCTCACCGG 
TTTAATGTGG GCCTGAACAT GAGAGCCACC AAGTGCGCCG TGTGTCTGGA TACTGTGCAC 
TTTGGACGCC AGGCATCCAA ATGCCTCGAA TGTCAAGTGA TGTGTCACCC CAAATGCTCC 
ACCTGCTTGC CCGCTACCTG TGGCCTGCCA GCTGAATATG CCACACACTT CACTGAGGCC 
TTCTGCCGGG ACAAAATGAA CTCCCCGGGG CTCCAGAGCA AGGAGCCTGG CAGCAGCTTG 
CACCTGGAAG GGTGGATGAA AGTGCCCAGA AATAACAAAC GGGGACAGCA AGGCTGGGAC 
AGGAAGTACA TTGTTCTGGA GGGGTCAAAA GTCCTCATCT ATGACAATGA AGCCAGAGAA 
GCTGGACAGA GGCCGGTGGA AGAATTTGAG CTGTGCCTTC CTGACGGGGA CGTATCTATT 
CATGGTGCCG TTGGTGCTTC AGAACTTGCA AATACCGCCA AAGCAGATGT CCCATACATC 
CTGAAGATGG AGTCTCACCC ACACACCACC TGCTGGCCTG GGAGAACCCT CTACTTGCTA 
GCACCCAGCT TCCCCGACAA GCAGCGCTGG GTCACCGCCT TAGAATCTGT CGTCGCAGGT 
GGGAGAGTTT CTAGGGAAAA GGCCGAAGCC GATGCTAAAT TACTTGGAAA CTCTCTGCTG 
AAACTGGAGG GCGATGACCG GCTTGACATG AACTGCACCC TGCCCTTCAG TGACCAGGTA 
GTGCTGGTGG GCACGGAGGA AGGCCTCTAC GCCCTGAATG TCTTGAAAAA CTCCTTAACC 
CACATCCCAG GGATTGGCGC AGTCTTCCAA ATTTACATCA TCAAGGACCT GGAGAAGCTG 
CTCATGATAG CAGGGGAAGA GCGGGCTCTG TGCCTGGTGG ACGTGAAGAA GGTGAAGCAG 
TCCCTGGCGC AGTCACACCT GCCTGCCCAG CCCGACGTCT CCCCCAACAT ATTCGAAGCC 
GTCAAAGGCT GCCACTTGTT CGCTGCTGGC AAGATCGAGA ACAGCCTGTG CATCTGCGCC 
GCTATGCCAA GCAAAGTCGT CATCCTCCGC TACAATGACA ACCTCAGCAA GTACTGCATC 
CGCAAGGAGA TCGAGACCTC AGAGCCCTGC AGCTGTATCC ACTTCACCAA CTACAGCATC 
CTCATTGGGA CCAACAAATT CTATGAGATC GACATGAAGC AGTACACGCT TGACGAGTTC 
CTGGACAAGA ACGACCATTC CTTGGCACCT GCTGTGTTCG CCTCCTCGTC CAACAGCTTC 
CCTGTCTCCA TTGTGCAGGC GAACAGCGCC GGGCAGCGAG AAGAATACCT GCTGTGCTTC 
CACGAATTTG GGGTGTTCGT GGATTCTTAC GGAAGACGTA GCCGCACAGA TGATCTCAAG 
TGGAGTCGCT TACCTCTGGC CTTCGCCTAC AGAGAACCTT ATCTGTTTGT GACTCACTTC 
AACTCCCTGG AAGTCATTGA GATCCAGGCC AGATCCTCAC TGGGGAGCCC TGCCCGAGCA 
TATCTGGAAA TTCCAAACCC TCGCTACCTG GGCCCCGCGA TTTCCTCCGG AGCGATTTAC 
CTGGCCTCCT CATACCAGGA CAAGTTAAGG GTCATATGCT GCAAAGGAAA CCTCGTGAAG 
GAGTCAGGCA CTGAGCAGCA CCGGGTGCCC TCCACCTCCC GCAGGTACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006530216.1
CDS	223..3753
Translation	MMQRHEEEAHEKGKILSEQKAMINAMDSKIRSLEQRIVELSEANKLAANSSLFTQRNMKAQEEMISELRQQKFYLETQAGKLEAQNRKLEEQLEKISHQDHSDKSRLLELETRLREVSLEHEEQKLELKRQLTELQLSLQERESQLTALQAARAALESQLRQAKTELEETTAEAEEEIQALTAHRDEIQRKFDALRNSCTVITDLEEQLNQLTEDNAELNNQNFYLSKQLDEASGANDEIVQLRSEVDHLRREITEREMQLTSQKQTMEALKTTCTMLEEQVLDLEALNDELLEKERQWEAWRSVLGDEKSQFECRVRELQRMLDTEKQSRARADQRITESRQVVELAVKEHKAEILALQQALKEQKLKAESLSDKLNDLEKKHAMLEMNARSLQQKLETERELKQRLLEEQAKLQQQMDLQKNHIFRLTQGLQEALDRADLLKTERSDLEYQLENIQVLYSHEKVKMEGTISQQTKLIDFLQAKMDQPAKKKKGLFSRRKEDPALPTQVPLQYNELKLALEKEKARCAELEEALQKTRIELRSAREEAAHRKATDHPHPSTPATARQQIAMSAIVRSPEHQPSAMSLLAPPSSRRKESSTPEEFSRRLKERMHHNIPHRFNVGLNMRATKCAVCLDTVHFGRQASKCLECQVMCHPKCSTCLPATCGLPAEYATHFTEAFCRDKMNSPGLQSKEPGSSLHLEGWMKVPRNNKRGQQGWDRKYIVLEGSKVLIYDNEAREAGQRPVEEFELCLPDGDVSIHGAVGASELANTAKADVPYILKMESHPHTTCWPGRTLYLLAPSFPDKQRWVTALESVVAGGRVSREKAEADAKLLGNSLLKLEGDDRLDMNCTLPFSDQVVLVGTEEGLYALNVLKNSLTHIPGIGAVFQIYIIKDLEKLLMIAGEERALCLVDVKKVKQSLAQSHLPAQPDVSPNIFEAVKGCHLFAAGKIENSLCICAAMPSKVVILRYNDNLSKYCIRKEIETSEPCSCIHFTNYSILIGTNKFYEIDMKQYTLDEFLDKNDHSLAPAVFASSSNSFPVSIVQANSAGQREEYLLCFHEFGVFVDSYGRRSRTDDLKWSRLPLAFAYREPYLFVTHFNSLEVIEIQARSSLGSPARAYLEIPNPRYLGPAISSGAIYLASSYQDKLRVICCKGNLVKESGTEQHRVPSTSRRY

Target ORF information:

RefSeq Version	XM_006530153.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus citron (Cit), transcript variant X33, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006530153.1

ORF Insert Sequence:

ATGATGCAGA GACACGAGGA GGAGGCCCAC GAGAAGGGCA AGATCCTCAG CGAGCAGAAG 
GCGATGATCA ACGCGATGGA TTCCAAGATC CGATCCCTGG AGCAGAGGAT CGTGGAGCTG 
TCGGAAGCCA ACAAGCTTGC GGCAAACAGC AGTCTCTTCA CCCAGAGGAA CATGAAGGCC 
CAGGAAGAGA TGATCTCAGA ACTCAGGCAG CAGAAATTTT ACCTGGAGAC GCAGGCCGGA 
AAGCTGGAGG CCCAGAACCG AAAGCTGGAA GAGCAACTGG AGAAGATCAG CCACCAAGAT 
CACAGTGACA AGAGTCGGCT GCTGGAGCTG GAAACAAGGC TGAGGGAGGT CAGCCTGGAG 
CACGAGGAGC AGAAGCTGGA GCTGAAGCGG CAGCTCACGG AGCTGCAGCT GTCCCTGCAG 
GAGCGCGAGT CCCAGCTGAC GGCCCTGCAG GCCGCCCGGG CAGCGCTGGA GAGCCAGCTC 
CGCCAGGCGA AGACAGAGCT GGAGGAGACA ACCGCGGAAG CGGAGGAGGA GATCCAGGCG 
CTCACGGCAC ATCGAGATGA GATCCAGCGC AAATTCGATG CCCTTCGCAA CAGCTGCACG 
GTCATCACCG ACCTGGAGGA GCAGCTGAAC CAGCTCACCG AGGACAACGC CGAGCTCAAC 
AACCAAAACT TCTACCTGTC CAAACAACTC GATGAGGCTT CCGGGGCCAA TGACGAGATT 
GTGCAGCTGC GGAGTGAGGT GGACCATCTC CGCCGTGAGA TCACGGAGCG GGAGATGCAG 
CTCACCAGCC AGAAGCAAAC GATGGAGGCT CTGAAGACGA CATGCACCAT GCTGGAGGAG 
CAGGTCCTGG ACCTGGAGGC CCTGAACGAC GAGCTGCTGG AGAAGGAGCG CCAGTGGGAG 
GCCTGGCGGA GCGTCCTCGG CGACGAGAAG TCCCAGTTCG AGTGTCGAGT TCGAGAGCTA 
CAGAGGATGC TGGACACCGA GAAGCAGAGC AGGGCTAGGG CCGATCAGCG GATCACCGAG 
TCGCGCCAGG TGGTGGAGTT GGCGGTGAAG GAACACAAGG CCGAGATTCT TGCTCTGCAG 
CAGGCTCTCA AGGAGCAGAA GCTCAAAGCC GAGAGCCTGT CGGACAAGCT CAACGACCTG 
GAGAAGAAGC ACGCCATGCT GGAGATGAAC GCCCGGAGCT TACAGCAGAA ACTAGAGACA 
GAGCGGGAGC TCAAACAGAG GCTTCTGGAG GAGCAAGCCA AATTGCAGCA GCAGATGGAC 
CTGCAGAAGA ACCACATCTT CAGACTGACG CAAGGGCTGC AGGAGGCGCT GGACCGGGCG 
GATCTGCTGA AGACAGAAAG GAGCGACCTG GAGTACCAGC TGGAAAACAT TCAGGTTCTC 
TACTCTCACG AGAAAGTGAA AATGGAAGGC ACAATCTCTC AGCAAACCAA ACTCATTGAT 
TTCCTGCAAG CCAAAATGGA CCAGCCTGCT AAAAAGAAAA AGGGTTTATT TAGTCGACGG 
AAAGAGGACC CTGCTTTGCC CACACAGGTT CCTCTGCAGT ACAATGAGCT GAAGCTAGCC 
CTGGAAAAGG AGAAAGCCCG ATGCGCGGAG CTGGAGGAGG CCCTTCAGAA GACCCGCATC 
GAACTCCGCT CTGCGCGGGA GGAAGCTGCC CACCGCAAAG CCACGGACCA CCCGCACCCG 
TCTACGCCAG CCACTGCGAG GCAGCAGATC GCCATGTCTG CCATTGTGCG GTCGCCCGAG 
CACCAGCCCA GTGCCATGAG CCTGCTTGCC CCACCATCCA GCCGCAGAAA GGAGTCGTCA 
ACTCCAGAGG AATTCAGCCG GCGTCTGAAA GAGCGCATGC ACCACAACAT CCCTCACCGG 
TTTAATGTGG GCCTGAACAT GAGAGCCACC AAGTGCGCCG TGTGTCTGGA TACTGTGCAC 
TTTGGACGCC AGGCATCCAA ATGCCTCGAA TGTCAAGTGA TGTGTCACCC CAAATGCTCC 
ACCTGCTTGC CCGCTACCTG TGGCCTGCCA GCTGAATATG CCACACACTT CACTGAGGCC 
TTCTGCCGGG ACAAAATGAA CTCCCCGGGG CTCCAGAGCA AGGAGCCTGG CAGCAGCTTG 
CACCTGGAAG GGTGGATGAA AGTGCCCAGA AATAACAAAC GGGGACAGCA AGGCTGGGAC 
AGGAAGTACA TTGTTCTGGA GGGGTCAAAA GTCCTCATCT ATGACAATGA AGCCAGAGAA 
GCTGGACAGA GGCCGGTGGA AGAATTTGAG CTGTGCCTTC CTGACGGGGA CGTATCTATT 
CATGGTGCCG TTGGTGCTTC AGAACTTGCA AATACCGCCA AAGCAGATGT CCCATACATC 
CTGAAGATGG AGTCTCACCC ACACACCACC TGCTGGCCTG GGAGAACCCT CTACTTGCTA 
GCACCCAGCT TCCCCGACAA GCAGCGCTGG GTCACCGCCT TAGAATCTGT CGTCGCAGGT 
GGGAGAGTTT CTAGGGAAAA GGCCGAAGCC GATGCTAAAT TACTTGGAAA CTCTCTGCTG 
AAACTGGAGG GCGATGACCG GCTTGACATG AACTGCACCC TGCCCTTCAG TGACCAGGTA 
GTGCTGGTGG GCACGGAGGA AGGCCTCTAC GCCCTGAATG TCTTGAAAAA CTCCTTAACC 
CACATCCCAG GGATTGGCGC AGTCTTCCAA ATTTACATCA TCAAGGACCT GGAGAAGCTG 
CTCATGATAG CAGGGGAAGA GCGGGCTCTG TGCCTGGTGG ACGTGAAGAA GGTGAAGCAG 
TCCCTGGCGC AGTCACACCT GCCTGCCCAG CCCGACGTCT CCCCCAACAT ATTCGAAGCC 
GTCAAAGGCT GCCACTTGTT CGCTGCTGGC AAGATCGAGA ACAGCCTGTG CATCTGCGCC 
GCTATGCCAA GCAAAGTCGT CATCCTCCGC TACAATGACA ACCTCAGCAA GTACTGCATC 
CGCAAGGAGA TCGAGACCTC AGAGCCCTGC AGCTGTATCC ACTTCACCAA CTACAGCATC 
CTCATTGGGA CCAACAAATT CTATGAGATC GACATGAAGC AGTACACGCT TGACGAGTTC 
CTGGACAAGA ACGACCATTC CTTGGCACCT GCTGTGTTCG CCTCCTCGTC CAACAGCTTC 
CCTGTCTCCA TTGTGCAGGC GAACAGCGCC GGGCAGCGAG AAGAATACCT GCTGTGCTTC 
CACGAATTTG GGGTGTTCGT GGATTCTTAC GGAAGACGTA GCCGCACAGA TGATCTCAAG 
TGGAGTCGCT TACCTCTGGC CTTCGCCTAC AGAGAACCTT ATCTGTTTGT GACTCACTTC 
AACTCCCTGG AAGTCATTGA GATCCAGGCC AGATCCTCAC TGGGGAGCCC TGCCCGAGCA 
TATCTGGAAA TTCCAAACCC TCGCTACCTG GGCCCCGCGA TTTCCTCCGG AGCGATTTAC 
CTGGCCTCCT CATACCAGGA CAAGTTAAGG GTCATATGCT GCAAAGGAAA CCTCGTGAAG 
GAGTCAGGCA CTGAGCAGCA CCGGGTGCCC TCCACCTCCC GCAGGTACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cit ( XM_006530147.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006530210.1 Mus musculus citron (Cit), transcript variant X22, mRNA.

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CloneID	OMu35787
Clone ID Related Accession (Same CDS sequence)	XM_006530148.3 , XM_006530147.2 , XM_006530148.4 , XM_006530147.1
Accession Version	XM_006530147.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5037bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	citron Rho-interacting kinase isoform X18
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000071.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006530147.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAAAGA AACTTCTCAT CAAAAGCAAA GAGCTCCAAG ACTCCCAGGA CAAGTGTCAC 
AAGATGGAGC AGGAAATGAC CCGGTTACAT CGCAGAGTGT CAGAGGTGGA GGCTGTGCTT 
AGTCAGAAGG AGGTGGAGCT GAAGGCCTCT GAGACTCAGA GATCCCTCCT GGAGCAGGAC 
CTTGCTACCT ACATCACAGA ATGCAGTAGC TTAAAGCGAA GTTTGGAGCA AGCGCGGATG 
GAGGTGTCCC AGGAGGATGA CAAAGCTCTG CAGCTTCTCC ACGACATCCG AGAGCAGAGC 
CGGAAGCTCC AGGAGATCAA GGAGCAGGAG TACCAGGCTC AGGTGGAGGA GATGAGGCTG 
ATGATGAATC AGCTGGAAGA AGACCTGGTG TCAGCCCGCA GACGCAGCGA TCTCTACGAG 
TCTGAGCTGA GGGAGTCTCG GCTTGCCGCC GAGGAATTCA AGCGGAAGGC AAACGAATGT 
CAGCACAAAC TGATGAAGGT AGTCAGCCAC CCTCCAAGAG GGGACTCCGG GGGCACGGCC 
CTGGATGATC TTCACAAGAT GCAAGGCCAC GCTGGCTTAA CGAGTGCTAA GGACCAAGGG 
AAGCCTGAAG TGGGAGAATA TTCCAAACTG GAGAAGATCA ATGCTGAGCA GCAGCTGAAG 
ATCCAGGAGC TCCAGGAGAA GCTGGAGAAG GCTGTAAAAG CCAGCACAGA GGCCACCGAG 
CTCCTGCAGA ATATCCGCCA GGCAAAGGAG CGAGCAGAGC GGGAGCTAGA GAAGCTACAC 
AACCGGGAAG ACTCCTCCGA GGGCATCAAA AAGAAGCTGG TGGAAGCCGA GGAACGCCGC 
CACTCCCTGG AGAACAAGGT AAAGAGACTA GAGACCATGG AGCGTAGAGA GAACAGACTG 
AAGGATGACA TCCAGACAAA GTCCGAACAG ATCCAGCAGA TGGCTGATAA AATTCTGGAG 
CTGGAGGAGA AACATCGGGA GGCTCAGGTC TCAGCTCAAC ATCTAGAAGT ACACTTGAAA 
CAGAAAGAAC AGCACTACGA GGAAAAGATC AAGGTATTGG ACAATCAGAT AAAGAAGGAC 
CTGGCCGACA AGGAGAGCCT GGAGAACATG ATGCAGAGAC ACGAGGAGGA GGCCCACGAG 
AAGGGCAAGA TCCTCAGCGA GCAGAAGGCG ATGATCAACG CGATGGATTC CAAGATCCGA 
TCCCTGGAGC AGAGGATCGT GGAGCTGTCG GAAGCCAACA AGCTTGCGGC AAACAGCAGT 
CTCTTCACCC AGAGGAACAT GAAGGCCCAG GAAGAGATGA TCTCAGAACT CAGGCAGCAG 
AAATTTTACC TGGAGACGCA GGCCGGAAAG CTGGAGGCCC AGAACCGAAA GCTGGAAGAG 
CAACTGGAGA AGATCAGCCA CCAAGATCAC AGTGACAAGA GTCGGCTGCT GGAGCTGGAA 
ACAAGGCTGA GGGAGGTCAG CCTGGAGCAC GAGGAGCAGA AGCTGGAGCT GAAGCGGCAG 
CTCACGGAGC TGCAGCTGTC CCTGCAGGAG CGCGAGTCCC AGCTGACGGC CCTGCAGGCC 
GCCCGGGCAG CGCTGGAGAG CCAGCTCCGC CAGGCGAAGA CAGAGCTGGA GGAGACAACC 
GCGGAAGCGG AGGAGGAGAT CCAGGCGCTC ACGGCACATC GAGATGAGAT CCAGCGCAAA 
TTCGATGCCC TTCGCAACAG CTGCACGGTC ATCACCGACC TGGAGGAGCA GCTGAACCAG 
CTCACCGAGG ACAACGCCGA GCTCAACAAC CAAAACTTCT ACCTGTCCAA ACAACTCGAT 
GAGGCTTCCG GGGCCAATGA CGAGATTGTG CAGCTGCGGA GTGAGGTGGA CCATCTCCGC 
CGTGAGATCA CGGAGCGGGA GATGCAGCTC ACCAGCCAGA AGCAAGCACA ACTCAGTGCT 
CCTGATTTGC AGACGATGGA GGCTCTGAAG ACGACATGCA CCATGCTGGA GGAGCAGGTC 
CTGGACCTGG AGGCCCTGAA CGACGAGCTG CTGGAGAAGG AGCGCCAGTG GGAGGCCTGG 
CGGAGCGTCC TCGGCGACGA GAAGTCCCAG TTCGAGTGTC GAGTTCGAGA GCTACAGAGG 
ATGCTGGACA CCGAGAAGCA GAGCAGGGCT AGGGCCGATC AGCGGATCAC CGAGTCGCGC 
CAGGTGGTGG AGTTGGCGGT GAAGGAACAC AAGGCCGAGA TTCTTGCTCT GCAGCAGGCT 
CTCAAGGAGC AGAAGCTCAA AGCCGAGAGC CTGTCGGACA AGCTCAACGA CCTGGAGAAG 
AAGCACGCCA TGCTGGAGAT GAACGCCCGG AGCTTACAGC AGAAACTAGA GACAGAGCGG 
GAGCTCAAAC AGAGGCTTCT GGAGGAGCAA GCCAAATTGC AGCAGCAGAT GGACCTGCAG 
AAGAACCACA TCTTCAGACT GACGCAAGGG CTGCAGGAGG CGCTGGACCG GGCGGATCTG 
CTGAAGACAG AAAGGAGCGA CCTGGAGTAC CAGCTGGAAA ACATTCAGGT TCTCTACTCT 
CACGAGAAAG TGAAAATGGA AGGCACAATC TCTCAGCAAA CCAAACTCAT TGATTTCCTG 
CAAGCCAAAA TGGACCAGCC TGCTAAAAAG AAAAAGGGTT TATTTAGTCG ACGGAAAGAG 
GACCCTGCTT TGCCCACACA GGTTCCTCTG CAGTACAATG AGCTGAAGCT AGCCCTGGAA 
AAGGAGAAAG CCCGATGCGC GGAGCTGGAG GAGGCCCTTC AGAAGACCCG CATCGAACTC 
CGCTCTGCGC GGGAGGAAGC TGCCCACCGC AAAGCCACGG ACCACCCGCA CCCGTCTACG 
CCAGCCACTG CGAGGCAGCA GATCGCCATG TCTGCCATTG TGCGGTCGCC CGAGCACCAG 
CCCAGTGCCA TGAGCCTGCT TGCCCCACCA TCCAGCCGCA GAAAGGAGTC GTCAACTCCA 
GAGGAATTCA GCCGGCGTCT GAAAGAGCGC ATGCACCACA ACATCCCTCA CCGGTTTAAT 
GTGGGCCTGA ACATGAGAGC CACCAAGTGC GCCGTGTGTC TGGATACTGT GCACTTTGGA 
CGCCAGGCAT CCAAATGCCT CGAATGTCAA GTGATGTGTC ACCCCAAATG CTCCACCTGC 
TTGCCCGCTA CCTGTGGCCT GCCAGCTGAA TATGCCACAC ACTTCACTGA GGCCTTCTGC 
CGGGACAAAA TGAACTCCCC GGGGCTCCAG AGCAAGGAGC CTGGCAGCAG CTTGCACCTG 
GAAGGGTGGA TGAAAGTGCC CAGAAATAAC AAACGGGGAC AGCAAGGCTG GGACAGGAAG 
TACATTGTTC TGGAGGGGTC AAAAGTCCTC ATCTATGACA ATGAAGCCAG AGAAGCTGGA 
CAGAGGCCGG TGGAAGAATT TGAGCTGTGC CTTCCTGACG GGGACGTATC TATTCATGGT 
GCCGTTGGTG CTTCAGAACT TGCAAATACC GCCAAAGCAG ATGTCCCATA CATCCTGAAG 
ATGGAGTCTC ACCCACACAC CACCTGCTGG CCTGGGAGAA CCCTCTACTT GCTAGCACCC 
AGCTTCCCCG ACAAGCAGCG CTGGGTCACC GCCTTAGAAT CTGTCGTCGC AGGTGGGAGA 
GTTTCTAGGG AAAAGGCCGA AGCCGATGCT GCACGTGACC ATACTTCGAG TGAGCATCAG 
CCAGTGTGGG TTGAGAAATT ACTTGGAAAC TCTCTGCTGA AACTGGAGGG CGATGACCGG 
CTTGACATGA ACTGCACCCT GCCCTTCAGT GACCAGGTAG TGCTGGTGGG CACGGAGGAA 
GGCCTCTACG CCCTGAATGT CTTGAAAAAC TCCTTAACCC ACATCCCAGG GATTGGCGCA 
GTCTTCCAAA TTTACATCAT CAAGGACCTG GAGAAGCTGC TCATGATAGC AGGGGAAGAG 
CGGGCTCTGT GCCTGGTGGA CGTGAAGAAG GTGAAGCAGT CCCTGGCGCA GTCACACCTG 
CCTGCCCAGC CCGACGTCTC CCCCAACATA TTCGAAGCCG TCAAAGGCTG CCACTTGTTC 
GCTGCTGGCA AGATCGAGAA CAGCCTGTGC ATCTGCGCCG CTATGCCAAG CAAAGTCGTC 
ATCCTCCGCT ACAATGACAA CCTCAGCAAG TACTGCATCC GCAAGGAGAT CGAGACCTCA 
GAGCCCTGCA GCTGTATCCA CTTCACCAAC TACAGCATCC TCATTGGGAC CAACAAATTC 
TATGAGATCG ACATGAAGCA GTACACGCTT GACGAGTTCC TGGACAAGAA CGACCATTCC 
TTGGCACCTG CTGTGTTCGC CTCCTCGTCC AACAGCTTCC CTGTCTCCAT TGTGCAGGCG 
AACAGCGCCG GGCAGCGAGA AGAATACCTG CTGTGCTTCC ACGAATTTGG GGTGTTCGTG 
GATTCTTACG GAAGACGTAG CCGCACAGAT GATCTCAAGT GGAGTCGCTT ACCTCTGGCC 
TTCGCCTACA GAGAACCTTA TCTGTTTGTG ACTCACTTCA ACTCCCTGGA AGTCATTGAG 
ATCCAGGCCA GATCCTCACT GGGGAGCCCT GCCCGAGCAT ATCTGGAAAT TCCAAACCCT 
CGCTACCTGG GCCCCGCGAT TTCCTCCGGA GCGATTTACC TGGCCTCCTC ATACCAGGAC 
AAGTTAAGGG TCATATGCTG CAAAGGAAAC CTCGTGAAGG AGTCAGGCAC TGAGCAGCAC 
CGGGTGCCCT CCACCTCCCG CAGCAGCCCC AACAAGCGAG GCCCACCAAC ATACAACGAG 
CACATCACCA AACGCGTGGC CTCCAGCCCG GCGCCACCGG AAGGCCCCAG CCACCCCCGA 
GAGCCAAGCA CACCGCACCG CTACCGAGAC AGAGAGGGTC GGACAGAGCT GCGCAGGGAC 
AAGTCTCCAG GCCGCCCTCT GGAGCGGGAG AAGTCCCCAG GCCGAATGCT CAGCACTAGG 
AGAGAGCGGT CCCCAGGGAG ACTGTTTGAA GACAGCAGCA GGGGCCGGCT GCCTGCAGGA 
GCAGTGAGGA CCCCACTGTC CCAGGTTAAC AAGGTCTGGG ACCAGTCTTC AGTATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006530210.1
CDS	316..5352
Translation	MEKKLLIKSKELQDSQDKCHKMEQEMTRLHRRVSEVEAVLSQKEVELKASETQRSLLEQDLATYITECSSLKRSLEQARMEVSQEDDKALQLLHDIREQSRKLQEIKEQEYQAQVEEMRLMMNQLEEDLVSARRRSDLYESELRESRLAAEEFKRKANECQHKLMKVVSHPPRGDSGGTALDDLHKMQGHAGLTSAKDQGKPEVGEYSKLEKINAEQQLKIQELQEKLEKAVKASTEATELLQNIRQAKERAERELEKLHNREDSSEGIKKKLVEAEERRHSLENKVKRLETMERRENRLKDDIQTKSEQIQQMADKILELEEKHREAQVSAQHLEVHLKQKEQHYEEKIKVLDNQIKKDLADKESLENMMQRHEEEAHEKGKILSEQKAMINAMDSKIRSLEQRIVELSEANKLAANSSLFTQRNMKAQEEMISELRQQKFYLETQAGKLEAQNRKLEEQLEKISHQDHSDKSRLLELETRLREVSLEHEEQKLELKRQLTELQLSLQERESQLTALQAARAALESQLRQAKTELEETTAEAEEEIQALTAHRDEIQRKFDALRNSCTVITDLEEQLNQLTEDNAELNNQNFYLSKQLDEASGANDEIVQLRSEVDHLRREITEREMQLTSQKQAQLSAPDLQTMEALKTTCTMLEEQVLDLEALNDELLEKERQWEAWRSVLGDEKSQFECRVRELQRMLDTEKQSRARADQRITESRQVVELAVKEHKAEILALQQALKEQKLKAESLSDKLNDLEKKHAMLEMNARSLQQKLETERELKQRLLEEQAKLQQQMDLQKNHIFRLTQGLQEALDRADLLKTERSDLEYQLENIQVLYSHEKVKMEGTISQQTKLIDFLQAKMDQPAKKKKGLFSRRKEDPALPTQVPLQYNELKLALEKEKARCAELEEALQKTRIELRSAREEAAHRKATDHPHPSTPATARQQIAMSAIVRSPEHQPSAMSLLAPPSSRRKESSTPEEFSRRLKERMHHNIPHRFNVGLNMRATKCAVCLDTVHFGRQASKCLECQVMCHPKCSTCLPATCGLPAEYATHFTEAFCRDKMNSPGLQSKEPGSSLHLEGWMKVPRNNKRGQQGWDRKYIVLEGSKVLIYDNEAREAGQRPVEEFELCLPDGDVSIHGAVGASELANTAKADVPYILKMESHPHTTCWPGRTLYLLAPSFPDKQRWVTALESVVAGGRVSREKAEADAARDHTSSEHQPVWVEKLLGNSLLKLEGDDRLDMNCTLPFSDQVVLVGTEEGLYALNVLKNSLTHIPGIGAVFQIYIIKDLEKLLMIAGEERALCLVDVKKVKQSLAQSHLPAQPDVSPNIFEAVKGCHLFAAGKIENSLCICAAMPSKVVILRYNDNLSKYCIRKEIETSEPCSCIHFTNYSILIGTNKFYEIDMKQYTLDEFLDKNDHSLAPAVFASSSNSFPVSIVQANSAGQREEYLLCFHEFGVFVDSYGRRSRTDDLKWSRLPLAFAYREPYLFVTHFNSLEVIEIQARSSLGSPARAYLEIPNPRYLGPAISSGAIYLASSYQDKLRVICCKGNLVKESGTEQHRVPSTSRSSPNKRGPPTYNEHITKRVASSPAPPEGPSHPREPSTPHRYRDREGRTELRRDKSPGRPLEREKSPGRMLSTRRERSPGRLFEDSSRGRLPAGAVRTPLSQVNKVWDQSSV

Target ORF information:

RefSeq Version	XM_006530147.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus citron (Cit), transcript variant X22, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006530147.2

ORF Insert Sequence:

ATGGAAAAGA AACTTCTCAT CAAAAGCAAA GAGCTCCAAG ACTCCCAGGA CAAGTGTCAC 
AAGATGGAGC AGGAAATGAC CCGGTTACAT CGCAGAGTGT CAGAGGTGGA GGCTGTGCTT 
AGTCAGAAGG AGGTGGAGCT GAAGGCCTCT GAGACTCAGA GATCCCTCCT GGAGCAGGAC 
CTTGCTACCT ACATCACAGA ATGCAGTAGC TTAAAGCGAA GTTTGGAGCA AGCGCGGATG 
GAGGTGTCCC AGGAGGATGA CAAAGCTCTG CAGCTTCTCC ACGACATCCG AGAGCAGAGC 
CGGAAGCTCC AGGAGATCAA GGAGCAGGAG TACCAGGCTC AGGTGGAGGA GATGAGGCTG 
ATGATGAATC AGCTGGAAGA AGACCTGGTG TCAGCCCGCA GACGCAGCGA TCTCTACGAG 
TCTGAGCTGA GGGAGTCTCG GCTTGCCGCC GAGGAATTCA AGCGGAAGGC AAACGAATGT 
CAGCACAAAC TGATGAAGGT AGTCAGCCAC CCTCCAAGAG GGGACTCCGG GGGCACGGCC 
CTGGATGATC TTCACAAGAT GCAAGGCCAC GCTGGCTTAA CGAGTGCTAA GGACCAAGGG 
AAGCCTGAAG TGGGAGAATA TTCCAAACTG GAGAAGATCA ATGCTGAGCA GCAGCTGAAG 
ATCCAGGAGC TCCAGGAGAA GCTGGAGAAG GCTGTAAAAG CCAGCACAGA GGCCACCGAG 
CTCCTGCAGA ATATCCGCCA GGCAAAGGAG CGAGCAGAGC GGGAGCTAGA GAAGCTACAC 
AACCGGGAAG ACTCCTCCGA GGGCATCAAA AAGAAGCTGG TGGAAGCCGA GGAACGCCGC 
CACTCCCTGG AGAACAAGGT AAAGAGACTA GAGACCATGG AGCGTAGAGA GAACAGACTG 
AAGGATGACA TCCAGACAAA GTCCGAACAG ATCCAGCAGA TGGCTGATAA AATTCTGGAG 
CTGGAGGAGA AACATCGGGA GGCTCAGGTC TCAGCTCAAC ATCTAGAAGT ACACTTGAAA 
CAGAAAGAAC AGCACTACGA GGAAAAGATC AAGGTATTGG ACAATCAGAT AAAGAAGGAC 
CTGGCCGACA AGGAGAGCCT GGAGAACATG ATGCAGAGAC ACGAGGAGGA GGCCCACGAG 
AAGGGCAAGA TCCTCAGCGA GCAGAAGGCG ATGATCAACG CGATGGATTC CAAGATCCGA 
TCCCTGGAGC AGAGGATCGT GGAGCTGTCG GAAGCCAACA AGCTTGCGGC AAACAGCAGT 
CTCTTCACCC AGAGGAACAT GAAGGCCCAG GAAGAGATGA TCTCAGAACT CAGGCAGCAG 
AAATTTTACC TGGAGACGCA GGCCGGAAAG CTGGAGGCCC AGAACCGAAA GCTGGAAGAG 
CAACTGGAGA AGATCAGCCA CCAAGATCAC AGTGACAAGA GTCGGCTGCT GGAGCTGGAA 
ACAAGGCTGA GGGAGGTCAG CCTGGAGCAC GAGGAGCAGA AGCTGGAGCT GAAGCGGCAG 
CTCACGGAGC TGCAGCTGTC CCTGCAGGAG CGCGAGTCCC AGCTGACGGC CCTGCAGGCC 
GCCCGGGCAG CGCTGGAGAG CCAGCTCCGC CAGGCGAAGA CAGAGCTGGA GGAGACAACC 
GCGGAAGCGG AGGAGGAGAT CCAGGCGCTC ACGGCACATC GAGATGAGAT CCAGCGCAAA 
TTCGATGCCC TTCGCAACAG CTGCACGGTC ATCACCGACC TGGAGGAGCA GCTGAACCAG 
CTCACCGAGG ACAACGCCGA GCTCAACAAC CAAAACTTCT ACCTGTCCAA ACAACTCGAT 
GAGGCTTCCG GGGCCAATGA CGAGATTGTG CAGCTGCGGA GTGAGGTGGA CCATCTCCGC 
CGTGAGATCA CGGAGCGGGA GATGCAGCTC ACCAGCCAGA AGCAAGCACA ACTCAGTGCT 
CCTGATTTGC AGACGATGGA GGCTCTGAAG ACGACATGCA CCATGCTGGA GGAGCAGGTC 
CTGGACCTGG AGGCCCTGAA CGACGAGCTG CTGGAGAAGG AGCGCCAGTG GGAGGCCTGG 
CGGAGCGTCC TCGGCGACGA GAAGTCCCAG TTCGAGTGTC GAGTTCGAGA GCTACAGAGG 
ATGCTGGACA CCGAGAAGCA GAGCAGGGCT AGGGCCGATC AGCGGATCAC CGAGTCGCGC 
CAGGTGGTGG AGTTGGCGGT GAAGGAACAC AAGGCCGAGA TTCTTGCTCT GCAGCAGGCT 
CTCAAGGAGC AGAAGCTCAA AGCCGAGAGC CTGTCGGACA AGCTCAACGA CCTGGAGAAG 
AAGCACGCCA TGCTGGAGAT GAACGCCCGG AGCTTACAGC AGAAACTAGA GACAGAGCGG 
GAGCTCAAAC AGAGGCTTCT GGAGGAGCAA GCCAAATTGC AGCAGCAGAT GGACCTGCAG 
AAGAACCACA TCTTCAGACT GACGCAAGGG CTGCAGGAGG CGCTGGACCG GGCGGATCTG 
CTGAAGACAG AAAGGAGCGA CCTGGAGTAC CAGCTGGAAA ACATTCAGGT TCTCTACTCT 
CACGAGAAAG TGAAAATGGA AGGCACAATC TCTCAGCAAA CCAAACTCAT TGATTTCCTG 
CAAGCCAAAA TGGACCAGCC TGCTAAAAAG AAAAAGGGTT TATTTAGTCG ACGGAAAGAG 
GACCCTGCTT TGCCCACACA GGTTCCTCTG CAGTACAATG AGCTGAAGCT AGCCCTGGAA 
AAGGAGAAAG CCCGATGCGC GGAGCTGGAG GAGGCCCTTC AGAAGACCCG CATCGAACTC 
CGCTCTGCGC GGGAGGAAGC TGCCCACCGC AAAGCCACGG ACCACCCGCA CCCGTCTACG 
CCAGCCACTG CGAGGCAGCA GATCGCCATG TCTGCCATTG TGCGGTCGCC CGAGCACCAG 
CCCAGTGCCA TGAGCCTGCT TGCCCCACCA TCCAGCCGCA GAAAGGAGTC GTCAACTCCA 
GAGGAATTCA GCCGGCGTCT GAAAGAGCGC ATGCACCACA ACATCCCTCA CCGGTTTAAT 
GTGGGCCTGA ACATGAGAGC CACCAAGTGC GCCGTGTGTC TGGATACTGT GCACTTTGGA 
CGCCAGGCAT CCAAATGCCT CGAATGTCAA GTGATGTGTC ACCCCAAATG CTCCACCTGC 
TTGCCCGCTA CCTGTGGCCT GCCAGCTGAA TATGCCACAC ACTTCACTGA GGCCTTCTGC 
CGGGACAAAA TGAACTCCCC GGGGCTCCAG AGCAAGGAGC CTGGCAGCAG CTTGCACCTG 
GAAGGGTGGA TGAAAGTGCC CAGAAATAAC AAACGGGGAC AGCAAGGCTG GGACAGGAAG 
TACATTGTTC TGGAGGGGTC AAAAGTCCTC ATCTATGACA ATGAAGCCAG AGAAGCTGGA 
CAGAGGCCGG TGGAAGAATT TGAGCTGTGC CTTCCTGACG GGGACGTATC TATTCATGGT 
GCCGTTGGTG CTTCAGAACT TGCAAATACC GCCAAAGCAG ATGTCCCATA CATCCTGAAG 
ATGGAGTCTC ACCCACACAC CACCTGCTGG CCTGGGAGAA CCCTCTACTT GCTAGCACCC 
AGCTTCCCCG ACAAGCAGCG CTGGGTCACC GCCTTAGAAT CTGTCGTCGC AGGTGGGAGA 
GTTTCTAGGG AAAAGGCCGA AGCCGATGCT GCACGTGACC ATACTTCGAG TGAGCATCAG 
CCAGTGTGGG TTGAGAAATT ACTTGGAAAC TCTCTGCTGA AACTGGAGGG CGATGACCGG 
CTTGACATGA ACTGCACCCT GCCCTTCAGT GACCAGGTAG TGCTGGTGGG CACGGAGGAA 
GGCCTCTACG CCCTGAATGT CTTGAAAAAC TCCTTAACCC ACATCCCAGG GATTGGCGCA 
GTCTTCCAAA TTTACATCAT CAAGGACCTG GAGAAGCTGC TCATGATAGC AGGGGAAGAG 
CGGGCTCTGT GCCTGGTGGA CGTGAAGAAG GTGAAGCAGT CCCTGGCGCA GTCACACCTG 
CCTGCCCAGC CCGACGTCTC CCCCAACATA TTCGAAGCCG TCAAAGGCTG CCACTTGTTC 
GCTGCTGGCA AGATCGAGAA CAGCCTGTGC ATCTGCGCCG CTATGCCAAG CAAAGTCGTC 
ATCCTCCGCT ACAATGACAA CCTCAGCAAG TACTGCATCC GCAAGGAGAT CGAGACCTCA 
GAGCCCTGCA GCTGTATCCA CTTCACCAAC TACAGCATCC TCATTGGGAC CAACAAATTC 
TATGAGATCG ACATGAAGCA GTACACGCTT GACGAGTTCC TGGACAAGAA CGACCATTCC 
TTGGCACCTG CTGTGTTCGC CTCCTCGTCC AACAGCTTCC CTGTCTCCAT TGTGCAGGCG 
AACAGCGCCG GGCAGCGAGA AGAATACCTG CTGTGCTTCC ACGAATTTGG GGTGTTCGTG 
GATTCTTACG GAAGACGTAG CCGCACAGAT GATCTCAAGT GGAGTCGCTT ACCTCTGGCC 
TTCGCCTACA GAGAACCTTA TCTGTTTGTG ACTCACTTCA ACTCCCTGGA AGTCATTGAG 
ATCCAGGCCA GATCCTCACT GGGGAGCCCT GCCCGAGCAT ATCTGGAAAT TCCAAACCCT 
CGCTACCTGG GCCCCGCGAT TTCCTCCGGA GCGATTTACC TGGCCTCCTC ATACCAGGAC 
AAGTTAAGGG TCATATGCTG CAAAGGAAAC CTCGTGAAGG AGTCAGGCAC TGAGCAGCAC 
CGGGTGCCCT CCACCTCCCG CAGCAGCCCC AACAAGCGAG GCCCACCAAC ATACAACGAG 
CACATCACCA AACGCGTGGC CTCCAGCCCG GCGCCACCGG AAGGCCCCAG CCACCCCCGA 
GAGCCAAGCA CACCGCACCG CTACCGAGAC AGAGAGGGTC GGACAGAGCT GCGCAGGGAC 
AAGTCTCCAG GCCGCCCTCT GGAGCGGGAG AAGTCCCCAG GCCGAATGCT CAGCACTAGG 
AGAGAGCGGT CCCCAGGGAG ACTGTTTGAA GACAGCAGCA GGGGCCGGCT GCCTGCAGGA 
GCAGTGAGGA CCCCACTGTC CCAGGTTAAC AAGGTCTGGG ACCAGTCTTC AGTATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cit ( XM_006530148.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006530211.1 Mus musculus citron (Cit), transcript variant X23, mRNA.

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CloneID	OMu35787
Clone ID Related Accession (Same CDS sequence)	XM_006530148.3 , XM_006530147.2 , XM_006530148.4 , XM_006530147.1
Accession Version	XM_006530148.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5037bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	citron Rho-interacting kinase isoform X18
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000071.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006530148.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAAAGA AACTTCTCAT CAAAAGCAAA GAGCTCCAAG ACTCCCAGGA CAAGTGTCAC 
AAGATGGAGC AGGAAATGAC CCGGTTACAT CGCAGAGTGT CAGAGGTGGA GGCTGTGCTT 
AGTCAGAAGG AGGTGGAGCT GAAGGCCTCT GAGACTCAGA GATCCCTCCT GGAGCAGGAC 
CTTGCTACCT ACATCACAGA ATGCAGTAGC TTAAAGCGAA GTTTGGAGCA AGCGCGGATG 
GAGGTGTCCC AGGAGGATGA CAAAGCTCTG CAGCTTCTCC ACGACATCCG AGAGCAGAGC 
CGGAAGCTCC AGGAGATCAA GGAGCAGGAG TACCAGGCTC AGGTGGAGGA GATGAGGCTG 
ATGATGAATC AGCTGGAAGA AGACCTGGTG TCAGCCCGCA GACGCAGCGA TCTCTACGAG 
TCTGAGCTGA GGGAGTCTCG GCTTGCCGCC GAGGAATTCA AGCGGAAGGC AAACGAATGT 
CAGCACAAAC TGATGAAGGT AGTCAGCCAC CCTCCAAGAG GGGACTCCGG GGGCACGGCC 
CTGGATGATC TTCACAAGAT GCAAGGCCAC GCTGGCTTAA CGAGTGCTAA GGACCAAGGG 
AAGCCTGAAG TGGGAGAATA TTCCAAACTG GAGAAGATCA ATGCTGAGCA GCAGCTGAAG 
ATCCAGGAGC TCCAGGAGAA GCTGGAGAAG GCTGTAAAAG CCAGCACAGA GGCCACCGAG 
CTCCTGCAGA ATATCCGCCA GGCAAAGGAG CGAGCAGAGC GGGAGCTAGA GAAGCTACAC 
AACCGGGAAG ACTCCTCCGA GGGCATCAAA AAGAAGCTGG TGGAAGCCGA GGAACGCCGC 
CACTCCCTGG AGAACAAGGT AAAGAGACTA GAGACCATGG AGCGTAGAGA GAACAGACTG 
AAGGATGACA TCCAGACAAA GTCCGAACAG ATCCAGCAGA TGGCTGATAA AATTCTGGAG 
CTGGAGGAGA AACATCGGGA GGCTCAGGTC TCAGCTCAAC ATCTAGAAGT ACACTTGAAA 
CAGAAAGAAC AGCACTACGA GGAAAAGATC AAGGTATTGG ACAATCAGAT AAAGAAGGAC 
CTGGCCGACA AGGAGAGCCT GGAGAACATG ATGCAGAGAC ACGAGGAGGA GGCCCACGAG 
AAGGGCAAGA TCCTCAGCGA GCAGAAGGCG ATGATCAACG CGATGGATTC CAAGATCCGA 
TCCCTGGAGC AGAGGATCGT GGAGCTGTCG GAAGCCAACA AGCTTGCGGC AAACAGCAGT 
CTCTTCACCC AGAGGAACAT GAAGGCCCAG GAAGAGATGA TCTCAGAACT CAGGCAGCAG 
AAATTTTACC TGGAGACGCA GGCCGGAAAG CTGGAGGCCC AGAACCGAAA GCTGGAAGAG 
CAACTGGAGA AGATCAGCCA CCAAGATCAC AGTGACAAGA GTCGGCTGCT GGAGCTGGAA 
ACAAGGCTGA GGGAGGTCAG CCTGGAGCAC GAGGAGCAGA AGCTGGAGCT GAAGCGGCAG 
CTCACGGAGC TGCAGCTGTC CCTGCAGGAG CGCGAGTCCC AGCTGACGGC CCTGCAGGCC 
GCCCGGGCAG CGCTGGAGAG CCAGCTCCGC CAGGCGAAGA CAGAGCTGGA GGAGACAACC 
GCGGAAGCGG AGGAGGAGAT CCAGGCGCTC ACGGCACATC GAGATGAGAT CCAGCGCAAA 
TTCGATGCCC TTCGCAACAG CTGCACGGTC ATCACCGACC TGGAGGAGCA GCTGAACCAG 
CTCACCGAGG ACAACGCCGA GCTCAACAAC CAAAACTTCT ACCTGTCCAA ACAACTCGAT 
GAGGCTTCCG GGGCCAATGA CGAGATTGTG CAGCTGCGGA GTGAGGTGGA CCATCTCCGC 
CGTGAGATCA CGGAGCGGGA GATGCAGCTC ACCAGCCAGA AGCAAGCACA ACTCAGTGCT 
CCTGATTTGC AGACGATGGA GGCTCTGAAG ACGACATGCA CCATGCTGGA GGAGCAGGTC 
CTGGACCTGG AGGCCCTGAA CGACGAGCTG CTGGAGAAGG AGCGCCAGTG GGAGGCCTGG 
CGGAGCGTCC TCGGCGACGA GAAGTCCCAG TTCGAGTGTC GAGTTCGAGA GCTACAGAGG 
ATGCTGGACA CCGAGAAGCA GAGCAGGGCT AGGGCCGATC AGCGGATCAC CGAGTCGCGC 
CAGGTGGTGG AGTTGGCGGT GAAGGAACAC AAGGCCGAGA TTCTTGCTCT GCAGCAGGCT 
CTCAAGGAGC AGAAGCTCAA AGCCGAGAGC CTGTCGGACA AGCTCAACGA CCTGGAGAAG 
AAGCACGCCA TGCTGGAGAT GAACGCCCGG AGCTTACAGC AGAAACTAGA GACAGAGCGG 
GAGCTCAAAC AGAGGCTTCT GGAGGAGCAA GCCAAATTGC AGCAGCAGAT GGACCTGCAG 
AAGAACCACA TCTTCAGACT GACGCAAGGG CTGCAGGAGG CGCTGGACCG GGCGGATCTG 
CTGAAGACAG AAAGGAGCGA CCTGGAGTAC CAGCTGGAAA ACATTCAGGT TCTCTACTCT 
CACGAGAAAG TGAAAATGGA AGGCACAATC TCTCAGCAAA CCAAACTCAT TGATTTCCTG 
CAAGCCAAAA TGGACCAGCC TGCTAAAAAG AAAAAGGGTT TATTTAGTCG ACGGAAAGAG 
GACCCTGCTT TGCCCACACA GGTTCCTCTG CAGTACAATG AGCTGAAGCT AGCCCTGGAA 
AAGGAGAAAG CCCGATGCGC GGAGCTGGAG GAGGCCCTTC AGAAGACCCG CATCGAACTC 
CGCTCTGCGC GGGAGGAAGC TGCCCACCGC AAAGCCACGG ACCACCCGCA CCCGTCTACG 
CCAGCCACTG CGAGGCAGCA GATCGCCATG TCTGCCATTG TGCGGTCGCC CGAGCACCAG 
CCCAGTGCCA TGAGCCTGCT TGCCCCACCA TCCAGCCGCA GAAAGGAGTC GTCAACTCCA 
GAGGAATTCA GCCGGCGTCT GAAAGAGCGC ATGCACCACA ACATCCCTCA CCGGTTTAAT 
GTGGGCCTGA ACATGAGAGC CACCAAGTGC GCCGTGTGTC TGGATACTGT GCACTTTGGA 
CGCCAGGCAT CCAAATGCCT CGAATGTCAA GTGATGTGTC ACCCCAAATG CTCCACCTGC 
TTGCCCGCTA CCTGTGGCCT GCCAGCTGAA TATGCCACAC ACTTCACTGA GGCCTTCTGC 
CGGGACAAAA TGAACTCCCC GGGGCTCCAG AGCAAGGAGC CTGGCAGCAG CTTGCACCTG 
GAAGGGTGGA TGAAAGTGCC CAGAAATAAC AAACGGGGAC AGCAAGGCTG GGACAGGAAG 
TACATTGTTC TGGAGGGGTC AAAAGTCCTC ATCTATGACA ATGAAGCCAG AGAAGCTGGA 
CAGAGGCCGG TGGAAGAATT TGAGCTGTGC CTTCCTGACG GGGACGTATC TATTCATGGT 
GCCGTTGGTG CTTCAGAACT TGCAAATACC GCCAAAGCAG ATGTCCCATA CATCCTGAAG 
ATGGAGTCTC ACCCACACAC CACCTGCTGG CCTGGGAGAA CCCTCTACTT GCTAGCACCC 
AGCTTCCCCG ACAAGCAGCG CTGGGTCACC GCCTTAGAAT CTGTCGTCGC AGGTGGGAGA 
GTTTCTAGGG AAAAGGCCGA AGCCGATGCT GCACGTGACC ATACTTCGAG TGAGCATCAG 
CCAGTGTGGG TTGAGAAATT ACTTGGAAAC TCTCTGCTGA AACTGGAGGG CGATGACCGG 
CTTGACATGA ACTGCACCCT GCCCTTCAGT GACCAGGTAG TGCTGGTGGG CACGGAGGAA 
GGCCTCTACG CCCTGAATGT CTTGAAAAAC TCCTTAACCC ACATCCCAGG GATTGGCGCA 
GTCTTCCAAA TTTACATCAT CAAGGACCTG GAGAAGCTGC TCATGATAGC AGGGGAAGAG 
CGGGCTCTGT GCCTGGTGGA CGTGAAGAAG GTGAAGCAGT CCCTGGCGCA GTCACACCTG 
CCTGCCCAGC CCGACGTCTC CCCCAACATA TTCGAAGCCG TCAAAGGCTG CCACTTGTTC 
GCTGCTGGCA AGATCGAGAA CAGCCTGTGC ATCTGCGCCG CTATGCCAAG CAAAGTCGTC 
ATCCTCCGCT ACAATGACAA CCTCAGCAAG TACTGCATCC GCAAGGAGAT CGAGACCTCA 
GAGCCCTGCA GCTGTATCCA CTTCACCAAC TACAGCATCC TCATTGGGAC CAACAAATTC 
TATGAGATCG ACATGAAGCA GTACACGCTT GACGAGTTCC TGGACAAGAA CGACCATTCC 
TTGGCACCTG CTGTGTTCGC CTCCTCGTCC AACAGCTTCC CTGTCTCCAT TGTGCAGGCG 
AACAGCGCCG GGCAGCGAGA AGAATACCTG CTGTGCTTCC ACGAATTTGG GGTGTTCGTG 
GATTCTTACG GAAGACGTAG CCGCACAGAT GATCTCAAGT GGAGTCGCTT ACCTCTGGCC 
TTCGCCTACA GAGAACCTTA TCTGTTTGTG ACTCACTTCA ACTCCCTGGA AGTCATTGAG 
ATCCAGGCCA GATCCTCACT GGGGAGCCCT GCCCGAGCAT ATCTGGAAAT TCCAAACCCT 
CGCTACCTGG GCCCCGCGAT TTCCTCCGGA GCGATTTACC TGGCCTCCTC ATACCAGGAC 
AAGTTAAGGG TCATATGCTG CAAAGGAAAC CTCGTGAAGG AGTCAGGCAC TGAGCAGCAC 
CGGGTGCCCT CCACCTCCCG CAGCAGCCCC AACAAGCGAG GCCCACCAAC ATACAACGAG 
CACATCACCA AACGCGTGGC CTCCAGCCCG GCGCCACCGG AAGGCCCCAG CCACCCCCGA 
GAGCCAAGCA CACCGCACCG CTACCGAGAC AGAGAGGGTC GGACAGAGCT GCGCAGGGAC 
AAGTCTCCAG GCCGCCCTCT GGAGCGGGAG AAGTCCCCAG GCCGAATGCT CAGCACTAGG 
AGAGAGCGGT CCCCAGGGAG ACTGTTTGAA GACAGCAGCA GGGGCCGGCT GCCTGCAGGA 
GCAGTGAGGA CCCCACTGTC CCAGGTTAAC AAGGTCTGGG ACCAGTCTTC AGTATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006530211.1
CDS	110..5146
Translation	MEKKLLIKSKELQDSQDKCHKMEQEMTRLHRRVSEVEAVLSQKEVELKASETQRSLLEQDLATYITECSSLKRSLEQARMEVSQEDDKALQLLHDIREQSRKLQEIKEQEYQAQVEEMRLMMNQLEEDLVSARRRSDLYESELRESRLAAEEFKRKANECQHKLMKVVSHPPRGDSGGTALDDLHKMQGHAGLTSAKDQGKPEVGEYSKLEKINAEQQLKIQELQEKLEKAVKASTEATELLQNIRQAKERAERELEKLHNREDSSEGIKKKLVEAEERRHSLENKVKRLETMERRENRLKDDIQTKSEQIQQMADKILELEEKHREAQVSAQHLEVHLKQKEQHYEEKIKVLDNQIKKDLADKESLENMMQRHEEEAHEKGKILSEQKAMINAMDSKIRSLEQRIVELSEANKLAANSSLFTQRNMKAQEEMISELRQQKFYLETQAGKLEAQNRKLEEQLEKISHQDHSDKSRLLELETRLREVSLEHEEQKLELKRQLTELQLSLQERESQLTALQAARAALESQLRQAKTELEETTAEAEEEIQALTAHRDEIQRKFDALRNSCTVITDLEEQLNQLTEDNAELNNQNFYLSKQLDEASGANDEIVQLRSEVDHLRREITEREMQLTSQKQAQLSAPDLQTMEALKTTCTMLEEQVLDLEALNDELLEKERQWEAWRSVLGDEKSQFECRVRELQRMLDTEKQSRARADQRITESRQVVELAVKEHKAEILALQQALKEQKLKAESLSDKLNDLEKKHAMLEMNARSLQQKLETERELKQRLLEEQAKLQQQMDLQKNHIFRLTQGLQEALDRADLLKTERSDLEYQLENIQVLYSHEKVKMEGTISQQTKLIDFLQAKMDQPAKKKKGLFSRRKEDPALPTQVPLQYNELKLALEKEKARCAELEEALQKTRIELRSAREEAAHRKATDHPHPSTPATARQQIAMSAIVRSPEHQPSAMSLLAPPSSRRKESSTPEEFSRRLKERMHHNIPHRFNVGLNMRATKCAVCLDTVHFGRQASKCLECQVMCHPKCSTCLPATCGLPAEYATHFTEAFCRDKMNSPGLQSKEPGSSLHLEGWMKVPRNNKRGQQGWDRKYIVLEGSKVLIYDNEAREAGQRPVEEFELCLPDGDVSIHGAVGASELANTAKADVPYILKMESHPHTTCWPGRTLYLLAPSFPDKQRWVTALESVVAGGRVSREKAEADAARDHTSSEHQPVWVEKLLGNSLLKLEGDDRLDMNCTLPFSDQVVLVGTEEGLYALNVLKNSLTHIPGIGAVFQIYIIKDLEKLLMIAGEERALCLVDVKKVKQSLAQSHLPAQPDVSPNIFEAVKGCHLFAAGKIENSLCICAAMPSKVVILRYNDNLSKYCIRKEIETSEPCSCIHFTNYSILIGTNKFYEIDMKQYTLDEFLDKNDHSLAPAVFASSSNSFPVSIVQANSAGQREEYLLCFHEFGVFVDSYGRRSRTDDLKWSRLPLAFAYREPYLFVTHFNSLEVIEIQARSSLGSPARAYLEIPNPRYLGPAISSGAIYLASSYQDKLRVICCKGNLVKESGTEQHRVPSTSRSSPNKRGPPTYNEHITKRVASSPAPPEGPSHPREPSTPHRYRDREGRTELRRDKSPGRPLEREKSPGRMLSTRRERSPGRLFEDSSRGRLPAGAVRTPLSQVNKVWDQSSV

Target ORF information:

RefSeq Version	XM_006530148.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus citron (Cit), transcript variant X23, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006530148.4

ORF Insert Sequence:

ATGGAAAAGA AACTTCTCAT CAAAAGCAAA GAGCTCCAAG ACTCCCAGGA CAAGTGTCAC 
AAGATGGAGC AGGAAATGAC CCGGTTACAT CGCAGAGTGT CAGAGGTGGA GGCTGTGCTT 
AGTCAGAAGG AGGTGGAGCT GAAGGCCTCT GAGACTCAGA GATCCCTCCT GGAGCAGGAC 
CTTGCTACCT ACATCACAGA ATGCAGTAGC TTAAAGCGAA GTTTGGAGCA AGCGCGGATG 
GAGGTGTCCC AGGAGGATGA CAAAGCTCTG CAGCTTCTCC ACGACATCCG AGAGCAGAGC 
CGGAAGCTCC AGGAGATCAA GGAGCAGGAG TACCAGGCTC AGGTGGAGGA GATGAGGCTG 
ATGATGAATC AGCTGGAAGA AGACCTGGTG TCAGCCCGCA GACGCAGCGA TCTCTACGAG 
TCTGAGCTGA GGGAGTCTCG GCTTGCCGCC GAGGAATTCA AGCGGAAGGC AAACGAATGT 
CAGCACAAAC TGATGAAGGT AGTCAGCCAC CCTCCAAGAG GGGACTCCGG GGGCACGGCC 
CTGGATGATC TTCACAAGAT GCAAGGCCAC GCTGGCTTAA CGAGTGCTAA GGACCAAGGG 
AAGCCTGAAG TGGGAGAATA TTCCAAACTG GAGAAGATCA ATGCTGAGCA GCAGCTGAAG 
ATCCAGGAGC TCCAGGAGAA GCTGGAGAAG GCTGTAAAAG CCAGCACAGA GGCCACCGAG 
CTCCTGCAGA ATATCCGCCA GGCAAAGGAG CGAGCAGAGC GGGAGCTAGA GAAGCTACAC 
AACCGGGAAG ACTCCTCCGA GGGCATCAAA AAGAAGCTGG TGGAAGCCGA GGAACGCCGC 
CACTCCCTGG AGAACAAGGT AAAGAGACTA GAGACCATGG AGCGTAGAGA GAACAGACTG 
AAGGATGACA TCCAGACAAA GTCCGAACAG ATCCAGCAGA TGGCTGATAA AATTCTGGAG 
CTGGAGGAGA AACATCGGGA GGCTCAGGTC TCAGCTCAAC ATCTAGAAGT ACACTTGAAA 
CAGAAAGAAC AGCACTACGA GGAAAAGATC AAGGTATTGG ACAATCAGAT AAAGAAGGAC 
CTGGCCGACA AGGAGAGCCT GGAGAACATG ATGCAGAGAC ACGAGGAGGA GGCCCACGAG 
AAGGGCAAGA TCCTCAGCGA GCAGAAGGCG ATGATCAACG CGATGGATTC CAAGATCCGA 
TCCCTGGAGC AGAGGATCGT GGAGCTGTCG GAAGCCAACA AGCTTGCGGC AAACAGCAGT 
CTCTTCACCC AGAGGAACAT GAAGGCCCAG GAAGAGATGA TCTCAGAACT CAGGCAGCAG 
AAATTTTACC TGGAGACGCA GGCCGGAAAG CTGGAGGCCC AGAACCGAAA GCTGGAAGAG 
CAACTGGAGA AGATCAGCCA CCAAGATCAC AGTGACAAGA GTCGGCTGCT GGAGCTGGAA 
ACAAGGCTGA GGGAGGTCAG CCTGGAGCAC GAGGAGCAGA AGCTGGAGCT GAAGCGGCAG 
CTCACGGAGC TGCAGCTGTC CCTGCAGGAG CGCGAGTCCC AGCTGACGGC CCTGCAGGCC 
GCCCGGGCAG CGCTGGAGAG CCAGCTCCGC CAGGCGAAGA CAGAGCTGGA GGAGACAACC 
GCGGAAGCGG AGGAGGAGAT CCAGGCGCTC ACGGCACATC GAGATGAGAT CCAGCGCAAA 
TTCGATGCCC TTCGCAACAG CTGCACGGTC ATCACCGACC TGGAGGAGCA GCTGAACCAG 
CTCACCGAGG ACAACGCCGA GCTCAACAAC CAAAACTTCT ACCTGTCCAA ACAACTCGAT 
GAGGCTTCCG GGGCCAATGA CGAGATTGTG CAGCTGCGGA GTGAGGTGGA CCATCTCCGC 
CGTGAGATCA CGGAGCGGGA GATGCAGCTC ACCAGCCAGA AGCAAGCACA ACTCAGTGCT 
CCTGATTTGC AGACGATGGA GGCTCTGAAG ACGACATGCA CCATGCTGGA GGAGCAGGTC 
CTGGACCTGG AGGCCCTGAA CGACGAGCTG CTGGAGAAGG AGCGCCAGTG GGAGGCCTGG 
CGGAGCGTCC TCGGCGACGA GAAGTCCCAG TTCGAGTGTC GAGTTCGAGA GCTACAGAGG 
ATGCTGGACA CCGAGAAGCA GAGCAGGGCT AGGGCCGATC AGCGGATCAC CGAGTCGCGC 
CAGGTGGTGG AGTTGGCGGT GAAGGAACAC AAGGCCGAGA TTCTTGCTCT GCAGCAGGCT 
CTCAAGGAGC AGAAGCTCAA AGCCGAGAGC CTGTCGGACA AGCTCAACGA CCTGGAGAAG 
AAGCACGCCA TGCTGGAGAT GAACGCCCGG AGCTTACAGC AGAAACTAGA GACAGAGCGG 
GAGCTCAAAC AGAGGCTTCT GGAGGAGCAA GCCAAATTGC AGCAGCAGAT GGACCTGCAG 
AAGAACCACA TCTTCAGACT GACGCAAGGG CTGCAGGAGG CGCTGGACCG GGCGGATCTG 
CTGAAGACAG AAAGGAGCGA CCTGGAGTAC CAGCTGGAAA ACATTCAGGT TCTCTACTCT 
CACGAGAAAG TGAAAATGGA AGGCACAATC TCTCAGCAAA CCAAACTCAT TGATTTCCTG 
CAAGCCAAAA TGGACCAGCC TGCTAAAAAG AAAAAGGGTT TATTTAGTCG ACGGAAAGAG 
GACCCTGCTT TGCCCACACA GGTTCCTCTG CAGTACAATG AGCTGAAGCT AGCCCTGGAA 
AAGGAGAAAG CCCGATGCGC GGAGCTGGAG GAGGCCCTTC AGAAGACCCG CATCGAACTC 
CGCTCTGCGC GGGAGGAAGC TGCCCACCGC AAAGCCACGG ACCACCCGCA CCCGTCTACG 
CCAGCCACTG CGAGGCAGCA GATCGCCATG TCTGCCATTG TGCGGTCGCC CGAGCACCAG 
CCCAGTGCCA TGAGCCTGCT TGCCCCACCA TCCAGCCGCA GAAAGGAGTC GTCAACTCCA 
GAGGAATTCA GCCGGCGTCT GAAAGAGCGC ATGCACCACA ACATCCCTCA CCGGTTTAAT 
GTGGGCCTGA ACATGAGAGC CACCAAGTGC GCCGTGTGTC TGGATACTGT GCACTTTGGA 
CGCCAGGCAT CCAAATGCCT CGAATGTCAA GTGATGTGTC ACCCCAAATG CTCCACCTGC 
TTGCCCGCTA CCTGTGGCCT GCCAGCTGAA TATGCCACAC ACTTCACTGA GGCCTTCTGC 
CGGGACAAAA TGAACTCCCC GGGGCTCCAG AGCAAGGAGC CTGGCAGCAG CTTGCACCTG 
GAAGGGTGGA TGAAAGTGCC CAGAAATAAC AAACGGGGAC AGCAAGGCTG GGACAGGAAG 
TACATTGTTC TGGAGGGGTC AAAAGTCCTC ATCTATGACA ATGAAGCCAG AGAAGCTGGA 
CAGAGGCCGG TGGAAGAATT TGAGCTGTGC CTTCCTGACG GGGACGTATC TATTCATGGT 
GCCGTTGGTG CTTCAGAACT TGCAAATACC GCCAAAGCAG ATGTCCCATA CATCCTGAAG 
ATGGAGTCTC ACCCACACAC CACCTGCTGG CCTGGGAGAA CCCTCTACTT GCTAGCACCC 
AGCTTCCCCG ACAAGCAGCG CTGGGTCACC GCCTTAGAAT CTGTCGTCGC AGGTGGGAGA 
GTTTCTAGGG AAAAGGCCGA AGCCGATGCT GCACGTGACC ATACTTCGAG TGAGCATCAG 
CCAGTGTGGG TTGAGAAATT ACTTGGAAAC TCTCTGCTGA AACTGGAGGG CGATGACCGG 
CTTGACATGA ACTGCACCCT GCCCTTCAGT GACCAGGTAG TGCTGGTGGG CACGGAGGAA 
GGCCTCTACG CCCTGAATGT CTTGAAAAAC TCCTTAACCC ACATCCCAGG GATTGGCGCA 
GTCTTCCAAA TTTACATCAT CAAGGACCTG GAGAAGCTGC TCATGATAGC AGGGGAAGAG 
CGGGCTCTGT GCCTGGTGGA CGTGAAGAAG GTGAAGCAGT CCCTGGCGCA GTCACACCTG 
CCTGCCCAGC CCGACGTCTC CCCCAACATA TTCGAAGCCG TCAAAGGCTG CCACTTGTTC 
GCTGCTGGCA AGATCGAGAA CAGCCTGTGC ATCTGCGCCG CTATGCCAAG CAAAGTCGTC 
ATCCTCCGCT ACAATGACAA CCTCAGCAAG TACTGCATCC GCAAGGAGAT CGAGACCTCA 
GAGCCCTGCA GCTGTATCCA CTTCACCAAC TACAGCATCC TCATTGGGAC CAACAAATTC 
TATGAGATCG ACATGAAGCA GTACACGCTT GACGAGTTCC TGGACAAGAA CGACCATTCC 
TTGGCACCTG CTGTGTTCGC CTCCTCGTCC AACAGCTTCC CTGTCTCCAT TGTGCAGGCG 
AACAGCGCCG GGCAGCGAGA AGAATACCTG CTGTGCTTCC ACGAATTTGG GGTGTTCGTG 
GATTCTTACG GAAGACGTAG CCGCACAGAT GATCTCAAGT GGAGTCGCTT ACCTCTGGCC 
TTCGCCTACA GAGAACCTTA TCTGTTTGTG ACTCACTTCA ACTCCCTGGA AGTCATTGAG 
ATCCAGGCCA GATCCTCACT GGGGAGCCCT GCCCGAGCAT ATCTGGAAAT TCCAAACCCT 
CGCTACCTGG GCCCCGCGAT TTCCTCCGGA GCGATTTACC TGGCCTCCTC ATACCAGGAC 
AAGTTAAGGG TCATATGCTG CAAAGGAAAC CTCGTGAAGG AGTCAGGCAC TGAGCAGCAC 
CGGGTGCCCT CCACCTCCCG CAGCAGCCCC AACAAGCGAG GCCCACCAAC ATACAACGAG 
CACATCACCA AACGCGTGGC CTCCAGCCCG GCGCCACCGG AAGGCCCCAG CCACCCCCGA 
GAGCCAAGCA CACCGCACCG CTACCGAGAC AGAGAGGGTC GGACAGAGCT GCGCAGGGAC 
AAGTCTCCAG GCCGCCCTCT GGAGCGGGAG AAGTCCCCAG GCCGAATGCT CAGCACTAGG 
AGAGAGCGGT CCCCAGGGAG ACTGTTTGAA GACAGCAGCA GGGGCCGGCT GCCTGCAGGA 
GCAGTGAGGA CCCCACTGTC CCAGGTTAAC AAGGTCTGGG ACCAGTCTTC AGTATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cit ( XM_006530149.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006530212.1 Mus musculus citron (Cit), transcript variant X24, mRNA.

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CloneID	OMu35788
Clone ID Related Accession (Same CDS sequence)	XM_006530149.4 , XM_006530149.3
Accession Version	XM_006530149.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4998bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	citron Rho-interacting kinase isoform X19
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000071.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006530149.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTCCTGG GCGAAGAAGC AATGATGGAG CAGGAAATGA CCCGGTTACA TCGCAGAGTG 
TCAGAGGTGG AGGCTGTGCT TAGTCAGAAG GAGGTGGAGC TGAAGGCCTC TGAGACTCAG 
AGATCCCTCC TGGAGCAGGA CCTTGCTACC TACATCACAG AATGCAGTAG CTTAAAGCGA 
AGTTTGGAGC AAGCGCGGAT GGAGGTGTCC CAGGAGGATG ACAAAGCTCT GCAGCTTCTC 
CACGACATCC GAGAGCAGAG CCGGAAGCTC CAGGAGATCA AGGAGCAGGA GTACCAGGCT 
CAGGTGGAGG AGATGAGGCT GATGATGAAT CAGCTGGAAG AAGACCTGGT GTCAGCCCGC 
AGACGCAGCG ATCTCTACGA GTCTGAGCTG AGGGAGTCTC GGCTTGCCGC CGAGGAATTC 
AAGCGGAAGG CAAACGAATG TCAGCACAAA CTGATGAAGG TAGTCAGCCA CCCTCCAAGA 
GGGGACTCCG GGGGCACGGC CCTGGATGAT CTTCACAAGA TGCAAGGCCA CGCTGGCTTA 
ACGAGTGCTA AGGACCAAGG GAAGCCTGAA GTGGGAGAAT ATTCCAAACT GGAGAAGATC 
AATGCTGAGC AGCAGCTGAA GATCCAGGAG CTCCAGGAGA AGCTGGAGAA GGCTGTAAAA 
GCCAGCACAG AGGCCACCGA GCTCCTGCAG AATATCCGCC AGGCAAAGGA GCGAGCAGAG 
CGGGAGCTAG AGAAGCTACA CAACCGGGAA GACTCCTCCG AGGGCATCAA AAAGAAGCTG 
GTGGAAGCCG AGGAACGCCG CCACTCCCTG GAGAACAAGG TAAAGAGACT AGAGACCATG 
GAGCGTAGAG AGAACAGACT GAAGGATGAC ATCCAGACAA AGTCCGAACA GATCCAGCAG 
ATGGCTGATA AAATTCTGGA GCTGGAGGAG AAACATCGGG AGGCTCAGGT CTCAGCTCAA 
CATCTAGAAG TACACTTGAA ACAGAAAGAA CAGCACTACG AGGAAAAGAT CAAGGTATTG 
GACAATCAGA TAAAGAAGGA CCTGGCCGAC AAGGAGAGCC TGGAGAACAT GATGCAGAGA 
CACGAGGAGG AGGCCCACGA GAAGGGCAAG ATCCTCAGCG AGCAGAAGGC GATGATCAAC 
GCGATGGATT CCAAGATCCG ATCCCTGGAG CAGAGGATCG TGGAGCTGTC GGAAGCCAAC 
AAGCTTGCGG CAAACAGCAG TCTCTTCACC CAGAGGAACA TGAAGGCCCA GGAAGAGATG 
ATCTCAGAAC TCAGGCAGCA GAAATTTTAC CTGGAGACGC AGGCCGGAAA GCTGGAGGCC 
CAGAACCGAA AGCTGGAAGA GCAACTGGAG AAGATCAGCC ACCAAGATCA CAGTGACAAG 
AGTCGGCTGC TGGAGCTGGA AACAAGGCTG AGGGAGGTCA GCCTGGAGCA CGAGGAGCAG 
AAGCTGGAGC TGAAGCGGCA GCTCACGGAG CTGCAGCTGT CCCTGCAGGA GCGCGAGTCC 
CAGCTGACGG CCCTGCAGGC CGCCCGGGCA GCGCTGGAGA GCCAGCTCCG CCAGGCGAAG 
ACAGAGCTGG AGGAGACAAC CGCGGAAGCG GAGGAGGAGA TCCAGGCGCT CACGGCACAT 
CGAGATGAGA TCCAGCGCAA ATTCGATGCC CTTCGCAACA GCTGCACGGT CATCACCGAC 
CTGGAGGAGC AGCTGAACCA GCTCACCGAG GACAACGCCG AGCTCAACAA CCAAAACTTC 
TACCTGTCCA AACAACTCGA TGAGGCTTCC GGGGCCAATG ACGAGATTGT GCAGCTGCGG 
AGTGAGGTGG ACCATCTCCG CCGTGAGATC ACGGAGCGGG AGATGCAGCT CACCAGCCAG 
AAGCAAGCAC AACTCAGTGC TCCTGATTTG CAGACGATGG AGGCTCTGAA GACGACATGC 
ACCATGCTGG AGGAGCAGGT CCTGGACCTG GAGGCCCTGA ACGACGAGCT GCTGGAGAAG 
GAGCGCCAGT GGGAGGCCTG GCGGAGCGTC CTCGGCGACG AGAAGTCCCA GTTCGAGTGT 
CGAGTTCGAG AGCTACAGAG GATGCTGGAC ACCGAGAAGC AGAGCAGGGC TAGGGCCGAT 
CAGCGGATCA CCGAGTCGCG CCAGGTGGTG GAGTTGGCGG TGAAGGAACA CAAGGCCGAG 
ATTCTTGCTC TGCAGCAGGC TCTCAAGGAG CAGAAGCTCA AAGCCGAGAG CCTGTCGGAC 
AAGCTCAACG ACCTGGAGAA GAAGCACGCC ATGCTGGAGA TGAACGCCCG GAGCTTACAG 
CAGAAACTAG AGACAGAGCG GGAGCTCAAA CAGAGGCTTC TGGAGGAGCA AGCCAAATTG 
CAGCAGCAGA TGGACCTGCA GAAGAACCAC ATCTTCAGAC TGACGCAAGG GCTGCAGGAG 
GCGCTGGACC GGGCGGATCT GCTGAAGACA GAAAGGAGCG ACCTGGAGTA CCAGCTGGAA 
AACATTCAGG TTCTCTACTC TCACGAGAAA GTGAAAATGG AAGGCACAAT CTCTCAGCAA 
ACCAAACTCA TTGATTTCCT GCAAGCCAAA ATGGACCAGC CTGCTAAAAA GAAAAAGGGT 
TTATTTAGTC GACGGAAAGA GGACCCTGCT TTGCCCACAC AGGTTCCTCT GCAGTACAAT 
GAGCTGAAGC TAGCCCTGGA AAAGGAGAAA GCCCGATGCG CGGAGCTGGA GGAGGCCCTT 
CAGAAGACCC GCATCGAACT CCGCTCTGCG CGGGAGGAAG CTGCCCACCG CAAAGCCACG 
GACCACCCGC ACCCGTCTAC GCCAGCCACT GCGAGGCAGC AGATCGCCAT GTCTGCCATT 
GTGCGGTCGC CCGAGCACCA GCCCAGTGCC ATGAGCCTGC TTGCCCCACC ATCCAGCCGC 
AGAAAGGAGT CGTCAACTCC AGAGGAATTC AGCCGGCGTC TGAAAGAGCG CATGCACCAC 
AACATCCCTC ACCGGTTTAA TGTGGGCCTG AACATGAGAG CCACCAAGTG CGCCGTGTGT 
CTGGATACTG TGCACTTTGG ACGCCAGGCA TCCAAATGCC TCGAATGTCA AGTGATGTGT 
CACCCCAAAT GCTCCACCTG CTTGCCCGCT ACCTGTGGCC TGCCAGCTGA ATATGCCACA 
CACTTCACTG AGGCCTTCTG CCGGGACAAA ATGAACTCCC CGGGGCTCCA GAGCAAGGAG 
CCTGGCAGCA GCTTGCACCT GGAAGGGTGG ATGAAAGTGC CCAGAAATAA CAAACGGGGA 
CAGCAAGGCT GGGACAGGAA GTACATTGTT CTGGAGGGGT CAAAAGTCCT CATCTATGAC 
AATGAAGCCA GAGAAGCTGG ACAGAGGCCG GTGGAAGAAT TTGAGCTGTG CCTTCCTGAC 
GGGGACGTAT CTATTCATGG TGCCGTTGGT GCTTCAGAAC TTGCAAATAC CGCCAAAGCA 
GATGTCCCAT ACATCCTGAA GATGGAGTCT CACCCACACA CCACCTGCTG GCCTGGGAGA 
ACCCTCTACT TGCTAGCACC CAGCTTCCCC GACAAGCAGC GCTGGGTCAC CGCCTTAGAA 
TCTGTCGTCG CAGGTGGGAG AGTTTCTAGG GAAAAGGCCG AAGCCGATGC TGCACGTGAC 
CATACTTCGA GTGAGCATCA GCCAGTGTGG GTTGAGAAAT TACTTGGAAA CTCTCTGCTG 
AAACTGGAGG GCGATGACCG GCTTGACATG AACTGCACCC TGCCCTTCAG TGACCAGGTA 
GTGCTGGTGG GCACGGAGGA AGGCCTCTAC GCCCTGAATG TCTTGAAAAA CTCCTTAACC 
CACATCCCAG GGATTGGCGC AGTCTTCCAA ATTTACATCA TCAAGGACCT GGAGAAGCTG 
CTCATGATAG CAGGGGAAGA GCGGGCTCTG TGCCTGGTGG ACGTGAAGAA GGTGAAGCAG 
TCCCTGGCGC AGTCACACCT GCCTGCCCAG CCCGACGTCT CCCCCAACAT ATTCGAAGCC 
GTCAAAGGCT GCCACTTGTT CGCTGCTGGC AAGATCGAGA ACAGCCTGTG CATCTGCGCC 
GCTATGCCAA GCAAAGTCGT CATCCTCCGC TACAATGACA ACCTCAGCAA GTACTGCATC 
CGCAAGGAGA TCGAGACCTC AGAGCCCTGC AGCTGTATCC ACTTCACCAA CTACAGCATC 
CTCATTGGGA CCAACAAATT CTATGAGATC GACATGAAGC AGTACACGCT TGACGAGTTC 
CTGGACAAGA ACGACCATTC CTTGGCACCT GCTGTGTTCG CCTCCTCGTC CAACAGCTTC 
CCTGTCTCCA TTGTGCAGGC GAACAGCGCC GGGCAGCGAG AAGAATACCT GCTGTGCTTC 
CACGAATTTG GGGTGTTCGT GGATTCTTAC GGAAGACGTA GCCGCACAGA TGATCTCAAG 
TGGAGTCGCT TACCTCTGGC CTTCGCCTAC AGAGAACCTT ATCTGTTTGT GACTCACTTC 
AACTCCCTGG AAGTCATTGA GATCCAGGCC AGATCCTCAC TGGGGAGCCC TGCCCGAGCA 
TATCTGGAAA TTCCAAACCC TCGCTACCTG GGCCCCGCGA TTTCCTCCGG AGCGATTTAC 
CTGGCCTCCT CATACCAGGA CAAGTTAAGG GTCATATGCT GCAAAGGAAA CCTCGTGAAG 
GAGTCAGGCA CTGAGCAGCA CCGGGTGCCC TCCACCTCCC GCAGCAGCCC CAACAAGCGA 
GGCCCACCAA CATACAACGA GCACATCACC AAACGCGTGG CCTCCAGCCC GGCGCCACCG 
GAAGGCCCCA GCCACCCCCG AGAGCCAAGC ACACCGCACC GCTACCGAGA CAGAGAGGGT 
CGGACAGAGC TGCGCAGGGA CAAGTCTCCA GGCCGCCCTC TGGAGCGGGA GAAGTCCCCA 
GGCCGAATGC TCAGCACTAG GAGAGAGCGG TCCCCAGGGA GACTGTTTGA AGACAGCAGC 
AGGGGCCGGC TGCCTGCAGG AGCAGTGAGG ACCCCACTGT CCCAGGTTAA CAAGGTCTGG 
GACCAGTCTT CAGTATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006530212.1
CDS	1147..6144
Translation	MLLGEEAMMEQEMTRLHRRVSEVEAVLSQKEVELKASETQRSLLEQDLATYITECSSLKRSLEQARMEVSQEDDKALQLLHDIREQSRKLQEIKEQEYQAQVEEMRLMMNQLEEDLVSARRRSDLYESELRESRLAAEEFKRKANECQHKLMKVVSHPPRGDSGGTALDDLHKMQGHAGLTSAKDQGKPEVGEYSKLEKINAEQQLKIQELQEKLEKAVKASTEATELLQNIRQAKERAERELEKLHNREDSSEGIKKKLVEAEERRHSLENKVKRLETMERRENRLKDDIQTKSEQIQQMADKILELEEKHREAQVSAQHLEVHLKQKEQHYEEKIKVLDNQIKKDLADKESLENMMQRHEEEAHEKGKILSEQKAMINAMDSKIRSLEQRIVELSEANKLAANSSLFTQRNMKAQEEMISELRQQKFYLETQAGKLEAQNRKLEEQLEKISHQDHSDKSRLLELETRLREVSLEHEEQKLELKRQLTELQLSLQERESQLTALQAARAALESQLRQAKTELEETTAEAEEEIQALTAHRDEIQRKFDALRNSCTVITDLEEQLNQLTEDNAELNNQNFYLSKQLDEASGANDEIVQLRSEVDHLRREITEREMQLTSQKQAQLSAPDLQTMEALKTTCTMLEEQVLDLEALNDELLEKERQWEAWRSVLGDEKSQFECRVRELQRMLDTEKQSRARADQRITESRQVVELAVKEHKAEILALQQALKEQKLKAESLSDKLNDLEKKHAMLEMNARSLQQKLETERELKQRLLEEQAKLQQQMDLQKNHIFRLTQGLQEALDRADLLKTERSDLEYQLENIQVLYSHEKVKMEGTISQQTKLIDFLQAKMDQPAKKKKGLFSRRKEDPALPTQVPLQYNELKLALEKEKARCAELEEALQKTRIELRSAREEAAHRKATDHPHPSTPATARQQIAMSAIVRSPEHQPSAMSLLAPPSSRRKESSTPEEFSRRLKERMHHNIPHRFNVGLNMRATKCAVCLDTVHFGRQASKCLECQVMCHPKCSTCLPATCGLPAEYATHFTEAFCRDKMNSPGLQSKEPGSSLHLEGWMKVPRNNKRGQQGWDRKYIVLEGSKVLIYDNEAREAGQRPVEEFELCLPDGDVSIHGAVGASELANTAKADVPYILKMESHPHTTCWPGRTLYLLAPSFPDKQRWVTALESVVAGGRVSREKAEADAARDHTSSEHQPVWVEKLLGNSLLKLEGDDRLDMNCTLPFSDQVVLVGTEEGLYALNVLKNSLTHIPGIGAVFQIYIIKDLEKLLMIAGEERALCLVDVKKVKQSLAQSHLPAQPDVSPNIFEAVKGCHLFAAGKIENSLCICAAMPSKVVILRYNDNLSKYCIRKEIETSEPCSCIHFTNYSILIGTNKFYEIDMKQYTLDEFLDKNDHSLAPAVFASSSNSFPVSIVQANSAGQREEYLLCFHEFGVFVDSYGRRSRTDDLKWSRLPLAFAYREPYLFVTHFNSLEVIEIQARSSLGSPARAYLEIPNPRYLGPAISSGAIYLASSYQDKLRVICCKGNLVKESGTEQHRVPSTSRSSPNKRGPPTYNEHITKRVASSPAPPEGPSHPREPSTPHRYRDREGRTELRRDKSPGRPLEREKSPGRMLSTRRERSPGRLFEDSSRGRLPAGAVRTPLSQVNKVWDQSSV

Target ORF information:

RefSeq Version	XM_006530149.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus citron (Cit), transcript variant X24, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006530149.4

ORF Insert Sequence:

ATGCTCCTGG GCGAAGAAGC AATGATGGAG CAGGAAATGA CCCGGTTACA TCGCAGAGTG 
TCAGAGGTGG AGGCTGTGCT TAGTCAGAAG GAGGTGGAGC TGAAGGCCTC TGAGACTCAG 
AGATCCCTCC TGGAGCAGGA CCTTGCTACC TACATCACAG AATGCAGTAG CTTAAAGCGA 
AGTTTGGAGC AAGCGCGGAT GGAGGTGTCC CAGGAGGATG ACAAAGCTCT GCAGCTTCTC 
CACGACATCC GAGAGCAGAG CCGGAAGCTC CAGGAGATCA AGGAGCAGGA GTACCAGGCT 
CAGGTGGAGG AGATGAGGCT GATGATGAAT CAGCTGGAAG AAGACCTGGT GTCAGCCCGC 
AGACGCAGCG ATCTCTACGA GTCTGAGCTG AGGGAGTCTC GGCTTGCCGC CGAGGAATTC 
AAGCGGAAGG CAAACGAATG TCAGCACAAA CTGATGAAGG TAGTCAGCCA CCCTCCAAGA 
GGGGACTCCG GGGGCACGGC CCTGGATGAT CTTCACAAGA TGCAAGGCCA CGCTGGCTTA 
ACGAGTGCTA AGGACCAAGG GAAGCCTGAA GTGGGAGAAT ATTCCAAACT GGAGAAGATC 
AATGCTGAGC AGCAGCTGAA GATCCAGGAG CTCCAGGAGA AGCTGGAGAA GGCTGTAAAA 
GCCAGCACAG AGGCCACCGA GCTCCTGCAG AATATCCGCC AGGCAAAGGA GCGAGCAGAG 
CGGGAGCTAG AGAAGCTACA CAACCGGGAA GACTCCTCCG AGGGCATCAA AAAGAAGCTG 
GTGGAAGCCG AGGAACGCCG CCACTCCCTG GAGAACAAGG TAAAGAGACT AGAGACCATG 
GAGCGTAGAG AGAACAGACT GAAGGATGAC ATCCAGACAA AGTCCGAACA GATCCAGCAG 
ATGGCTGATA AAATTCTGGA GCTGGAGGAG AAACATCGGG AGGCTCAGGT CTCAGCTCAA 
CATCTAGAAG TACACTTGAA ACAGAAAGAA CAGCACTACG AGGAAAAGAT CAAGGTATTG 
GACAATCAGA TAAAGAAGGA CCTGGCCGAC AAGGAGAGCC TGGAGAACAT GATGCAGAGA 
CACGAGGAGG AGGCCCACGA GAAGGGCAAG ATCCTCAGCG AGCAGAAGGC GATGATCAAC 
GCGATGGATT CCAAGATCCG ATCCCTGGAG CAGAGGATCG TGGAGCTGTC GGAAGCCAAC 
AAGCTTGCGG CAAACAGCAG TCTCTTCACC CAGAGGAACA TGAAGGCCCA GGAAGAGATG 
ATCTCAGAAC TCAGGCAGCA GAAATTTTAC CTGGAGACGC AGGCCGGAAA GCTGGAGGCC 
CAGAACCGAA AGCTGGAAGA GCAACTGGAG AAGATCAGCC ACCAAGATCA CAGTGACAAG 
AGTCGGCTGC TGGAGCTGGA AACAAGGCTG AGGGAGGTCA GCCTGGAGCA CGAGGAGCAG 
AAGCTGGAGC TGAAGCGGCA GCTCACGGAG CTGCAGCTGT CCCTGCAGGA GCGCGAGTCC 
CAGCTGACGG CCCTGCAGGC CGCCCGGGCA GCGCTGGAGA GCCAGCTCCG CCAGGCGAAG 
ACAGAGCTGG AGGAGACAAC CGCGGAAGCG GAGGAGGAGA TCCAGGCGCT CACGGCACAT 
CGAGATGAGA TCCAGCGCAA ATTCGATGCC CTTCGCAACA GCTGCACGGT CATCACCGAC 
CTGGAGGAGC AGCTGAACCA GCTCACCGAG GACAACGCCG AGCTCAACAA CCAAAACTTC 
TACCTGTCCA AACAACTCGA TGAGGCTTCC GGGGCCAATG ACGAGATTGT GCAGCTGCGG 
AGTGAGGTGG ACCATCTCCG CCGTGAGATC ACGGAGCGGG AGATGCAGCT CACCAGCCAG 
AAGCAAGCAC AACTCAGTGC TCCTGATTTG CAGACGATGG AGGCTCTGAA GACGACATGC 
ACCATGCTGG AGGAGCAGGT CCTGGACCTG GAGGCCCTGA ACGACGAGCT GCTGGAGAAG 
GAGCGCCAGT GGGAGGCCTG GCGGAGCGTC CTCGGCGACG AGAAGTCCCA GTTCGAGTGT 
CGAGTTCGAG AGCTACAGAG GATGCTGGAC ACCGAGAAGC AGAGCAGGGC TAGGGCCGAT 
CAGCGGATCA CCGAGTCGCG CCAGGTGGTG GAGTTGGCGG TGAAGGAACA CAAGGCCGAG 
ATTCTTGCTC TGCAGCAGGC TCTCAAGGAG CAGAAGCTCA AAGCCGAGAG CCTGTCGGAC 
AAGCTCAACG ACCTGGAGAA GAAGCACGCC ATGCTGGAGA TGAACGCCCG GAGCTTACAG 
CAGAAACTAG AGACAGAGCG GGAGCTCAAA CAGAGGCTTC TGGAGGAGCA AGCCAAATTG 
CAGCAGCAGA TGGACCTGCA GAAGAACCAC ATCTTCAGAC TGACGCAAGG GCTGCAGGAG 
GCGCTGGACC GGGCGGATCT GCTGAAGACA GAAAGGAGCG ACCTGGAGTA CCAGCTGGAA 
AACATTCAGG TTCTCTACTC TCACGAGAAA GTGAAAATGG AAGGCACAAT CTCTCAGCAA 
ACCAAACTCA TTGATTTCCT GCAAGCCAAA ATGGACCAGC CTGCTAAAAA GAAAAAGGGT 
TTATTTAGTC GACGGAAAGA GGACCCTGCT TTGCCCACAC AGGTTCCTCT GCAGTACAAT 
GAGCTGAAGC TAGCCCTGGA AAAGGAGAAA GCCCGATGCG CGGAGCTGGA GGAGGCCCTT 
CAGAAGACCC GCATCGAACT CCGCTCTGCG CGGGAGGAAG CTGCCCACCG CAAAGCCACG 
GACCACCCGC ACCCGTCTAC GCCAGCCACT GCGAGGCAGC AGATCGCCAT GTCTGCCATT 
GTGCGGTCGC CCGAGCACCA GCCCAGTGCC ATGAGCCTGC TTGCCCCACC ATCCAGCCGC 
AGAAAGGAGT CGTCAACTCC AGAGGAATTC AGCCGGCGTC TGAAAGAGCG CATGCACCAC 
AACATCCCTC ACCGGTTTAA TGTGGGCCTG AACATGAGAG CCACCAAGTG CGCCGTGTGT 
CTGGATACTG TGCACTTTGG ACGCCAGGCA TCCAAATGCC TCGAATGTCA AGTGATGTGT 
CACCCCAAAT GCTCCACCTG CTTGCCCGCT ACCTGTGGCC TGCCAGCTGA ATATGCCACA 
CACTTCACTG AGGCCTTCTG CCGGGACAAA ATGAACTCCC CGGGGCTCCA GAGCAAGGAG 
CCTGGCAGCA GCTTGCACCT GGAAGGGTGG ATGAAAGTGC CCAGAAATAA CAAACGGGGA 
CAGCAAGGCT GGGACAGGAA GTACATTGTT CTGGAGGGGT CAAAAGTCCT CATCTATGAC 
AATGAAGCCA GAGAAGCTGG ACAGAGGCCG GTGGAAGAAT TTGAGCTGTG CCTTCCTGAC 
GGGGACGTAT CTATTCATGG TGCCGTTGGT GCTTCAGAAC TTGCAAATAC CGCCAAAGCA 
GATGTCCCAT ACATCCTGAA GATGGAGTCT CACCCACACA CCACCTGCTG GCCTGGGAGA 
ACCCTCTACT TGCTAGCACC CAGCTTCCCC GACAAGCAGC GCTGGGTCAC CGCCTTAGAA 
TCTGTCGTCG CAGGTGGGAG AGTTTCTAGG GAAAAGGCCG AAGCCGATGC TGCACGTGAC 
CATACTTCGA GTGAGCATCA GCCAGTGTGG GTTGAGAAAT TACTTGGAAA CTCTCTGCTG 
AAACTGGAGG GCGATGACCG GCTTGACATG AACTGCACCC TGCCCTTCAG TGACCAGGTA 
GTGCTGGTGG GCACGGAGGA AGGCCTCTAC GCCCTGAATG TCTTGAAAAA CTCCTTAACC 
CACATCCCAG GGATTGGCGC AGTCTTCCAA ATTTACATCA TCAAGGACCT GGAGAAGCTG 
CTCATGATAG CAGGGGAAGA GCGGGCTCTG TGCCTGGTGG ACGTGAAGAA GGTGAAGCAG 
TCCCTGGCGC AGTCACACCT GCCTGCCCAG CCCGACGTCT CCCCCAACAT ATTCGAAGCC 
GTCAAAGGCT GCCACTTGTT CGCTGCTGGC AAGATCGAGA ACAGCCTGTG CATCTGCGCC 
GCTATGCCAA GCAAAGTCGT CATCCTCCGC TACAATGACA ACCTCAGCAA GTACTGCATC 
CGCAAGGAGA TCGAGACCTC AGAGCCCTGC AGCTGTATCC ACTTCACCAA CTACAGCATC 
CTCATTGGGA CCAACAAATT CTATGAGATC GACATGAAGC AGTACACGCT TGACGAGTTC 
CTGGACAAGA ACGACCATTC CTTGGCACCT GCTGTGTTCG CCTCCTCGTC CAACAGCTTC 
CCTGTCTCCA TTGTGCAGGC GAACAGCGCC GGGCAGCGAG AAGAATACCT GCTGTGCTTC 
CACGAATTTG GGGTGTTCGT GGATTCTTAC GGAAGACGTA GCCGCACAGA TGATCTCAAG 
TGGAGTCGCT TACCTCTGGC CTTCGCCTAC AGAGAACCTT ATCTGTTTGT GACTCACTTC 
AACTCCCTGG AAGTCATTGA GATCCAGGCC AGATCCTCAC TGGGGAGCCC TGCCCGAGCA 
TATCTGGAAA TTCCAAACCC TCGCTACCTG GGCCCCGCGA TTTCCTCCGG AGCGATTTAC 
CTGGCCTCCT CATACCAGGA CAAGTTAAGG GTCATATGCT GCAAAGGAAA CCTCGTGAAG 
GAGTCAGGCA CTGAGCAGCA CCGGGTGCCC TCCACCTCCC GCAGCAGCCC CAACAAGCGA 
GGCCCACCAA CATACAACGA GCACATCACC AAACGCGTGG CCTCCAGCCC GGCGCCACCG 
GAAGGCCCCA GCCACCCCCG AGAGCCAAGC ACACCGCACC GCTACCGAGA CAGAGAGGGT 
CGGACAGAGC TGCGCAGGGA CAAGTCTCCA GGCCGCCCTC TGGAGCGGGA GAAGTCCCCA 
GGCCGAATGC TCAGCACTAG GAGAGAGCGG TCCCCAGGGA GACTGTTTGA AGACAGCAGC 
AGGGGCCGGC TGCCTGCAGG AGCAGTGAGG ACCCCACTGT CCCAGGTTAA CAAGGTCTGG 
GACCAGTCTT CAGTATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cit ( XM_006530150.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006530213.1 Mus musculus citron (Cit), transcript variant X25, mRNA.

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CloneID	OMu35789
Clone ID Related Accession (Same CDS sequence)	XM_006530150.4 , XM_006530150.3
Accession Version	XM_006530150.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4974bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	citron Rho-interacting kinase isoform X20
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000071.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006530150.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGCAGG AAATGACCCG GTTACATCGC AGAGTGTCAG AGGTGGAGGC TGTGCTTAGT 
CAGAAGGAGG TGGAGCTGAA GGCCTCTGAG ACTCAGAGAT CCCTCCTGGA GCAGGACCTT 
GCTACCTACA TCACAGAATG CAGTAGCTTA AAGCGAAGTT TGGAGCAAGC GCGGATGGAG 
GTGTCCCAGG AGGATGACAA AGCTCTGCAG CTTCTCCACG ACATCCGAGA GCAGAGCCGG 
AAGCTCCAGG AGATCAAGGA GCAGGAGTAC CAGGCTCAGG TGGAGGAGAT GAGGCTGATG 
ATGAATCAGC TGGAAGAAGA CCTGGTGTCA GCCCGCAGAC GCAGCGATCT CTACGAGTCT 
GAGCTGAGGG AGTCTCGGCT TGCCGCCGAG GAATTCAAGC GGAAGGCAAA CGAATGTCAG 
CACAAACTGA TGAAGGTAGT CAGCCACCCT CCAAGAGGGG ACTCCGGGGG CACGGCCCTG 
GATGATCTTC ACAAGATGCA AGGCCACGCT GGCTTAACGA GTGCTAAGGA CCAAGGGAAG 
CCTGAAGTGG GAGAATATTC CAAACTGGAG AAGATCAATG CTGAGCAGCA GCTGAAGATC 
CAGGAGCTCC AGGAGAAGCT GGAGAAGGCT GTAAAAGCCA GCACAGAGGC CACCGAGCTC 
CTGCAGAATA TCCGCCAGGC AAAGGAGCGA GCAGAGCGGG AGCTAGAGAA GCTACACAAC 
CGGGAAGACT CCTCCGAGGG CATCAAAAAG AAGCTGGTGG AAGCCGAGGA ACGCCGCCAC 
TCCCTGGAGA ACAAGGTAAA GAGACTAGAG ACCATGGAGC GTAGAGAGAA CAGACTGAAG 
GATGACATCC AGACAAAGTC CGAACAGATC CAGCAGATGG CTGATAAAAT TCTGGAGCTG 
GAGGAGAAAC ATCGGGAGGC TCAGGTCTCA GCTCAACATC TAGAAGTACA CTTGAAACAG 
AAAGAACAGC ACTACGAGGA AAAGATCAAG GTATTGGACA ATCAGATAAA GAAGGACCTG 
GCCGACAAGG AGAGCCTGGA GAACATGATG CAGAGACACG AGGAGGAGGC CCACGAGAAG 
GGCAAGATCC TCAGCGAGCA GAAGGCGATG ATCAACGCGA TGGATTCCAA GATCCGATCC 
CTGGAGCAGA GGATCGTGGA GCTGTCGGAA GCCAACAAGC TTGCGGCAAA CAGCAGTCTC 
TTCACCCAGA GGAACATGAA GGCCCAGGAA GAGATGATCT CAGAACTCAG GCAGCAGAAA 
TTTTACCTGG AGACGCAGGC CGGAAAGCTG GAGGCCCAGA ACCGAAAGCT GGAAGAGCAA 
CTGGAGAAGA TCAGCCACCA AGATCACAGT GACAAGAGTC GGCTGCTGGA GCTGGAAACA 
AGGCTGAGGG AGGTCAGCCT GGAGCACGAG GAGCAGAAGC TGGAGCTGAA GCGGCAGCTC 
ACGGAGCTGC AGCTGTCCCT GCAGGAGCGC GAGTCCCAGC TGACGGCCCT GCAGGCCGCC 
CGGGCAGCGC TGGAGAGCCA GCTCCGCCAG GCGAAGACAG AGCTGGAGGA GACAACCGCG 
GAAGCGGAGG AGGAGATCCA GGCGCTCACG GCACATCGAG ATGAGATCCA GCGCAAATTC 
GATGCCCTTC GCAACAGCTG CACGGTCATC ACCGACCTGG AGGAGCAGCT GAACCAGCTC 
ACCGAGGACA ACGCCGAGCT CAACAACCAA AACTTCTACC TGTCCAAACA ACTCGATGAG 
GCTTCCGGGG CCAATGACGA GATTGTGCAG CTGCGGAGTG AGGTGGACCA TCTCCGCCGT 
GAGATCACGG AGCGGGAGAT GCAGCTCACC AGCCAGAAGC AAGCACAACT CAGTGCTCCT 
GATTTGCAGA CGATGGAGGC TCTGAAGACG ACATGCACCA TGCTGGAGGA GCAGGTCCTG 
GACCTGGAGG CCCTGAACGA CGAGCTGCTG GAGAAGGAGC GCCAGTGGGA GGCCTGGCGG 
AGCGTCCTCG GCGACGAGAA GTCCCAGTTC GAGTGTCGAG TTCGAGAGCT ACAGAGGATG 
CTGGACACCG AGAAGCAGAG CAGGGCTAGG GCCGATCAGC GGATCACCGA GTCGCGCCAG 
GTGGTGGAGT TGGCGGTGAA GGAACACAAG GCCGAGATTC TTGCTCTGCA GCAGGCTCTC 
AAGGAGCAGA AGCTCAAAGC CGAGAGCCTG TCGGACAAGC TCAACGACCT GGAGAAGAAG 
CACGCCATGC TGGAGATGAA CGCCCGGAGC TTACAGCAGA AACTAGAGAC AGAGCGGGAG 
CTCAAACAGA GGCTTCTGGA GGAGCAAGCC AAATTGCAGC AGCAGATGGA CCTGCAGAAG 
AACCACATCT TCAGACTGAC GCAAGGGCTG CAGGAGGCGC TGGACCGGGC GGATCTGCTG 
AAGACAGAAA GGAGCGACCT GGAGTACCAG CTGGAAAACA TTCAGGTTCT CTACTCTCAC 
GAGAAAGTGA AAATGGAAGG CACAATCTCT CAGCAAACCA AACTCATTGA TTTCCTGCAA 
GCCAAAATGG ACCAGCCTGC TAAAAAGAAA AAGGGTTTAT TTAGTCGACG GAAAGAGGAC 
CCTGCTTTGC CCACACAGGT TCCTCTGCAG TACAATGAGC TGAAGCTAGC CCTGGAAAAG 
GAGAAAGCCC GATGCGCGGA GCTGGAGGAG GCCCTTCAGA AGACCCGCAT CGAACTCCGC 
TCTGCGCGGG AGGAAGCTGC CCACCGCAAA GCCACGGACC ACCCGCACCC GTCTACGCCA 
GCCACTGCGA GGCAGCAGAT CGCCATGTCT GCCATTGTGC GGTCGCCCGA GCACCAGCCC 
AGTGCCATGA GCCTGCTTGC CCCACCATCC AGCCGCAGAA AGGAGTCGTC AACTCCAGAG 
GAATTCAGCC GGCGTCTGAA AGAGCGCATG CACCACAACA TCCCTCACCG GTTTAATGTG 
GGCCTGAACA TGAGAGCCAC CAAGTGCGCC GTGTGTCTGG ATACTGTGCA CTTTGGACGC 
CAGGCATCCA AATGCCTCGA ATGTCAAGTG ATGTGTCACC CCAAATGCTC CACCTGCTTG 
CCCGCTACCT GTGGCCTGCC AGCTGAATAT GCCACACACT TCACTGAGGC CTTCTGCCGG 
GACAAAATGA ACTCCCCGGG GCTCCAGAGC AAGGAGCCTG GCAGCAGCTT GCACCTGGAA 
GGGTGGATGA AAGTGCCCAG AAATAACAAA CGGGGACAGC AAGGCTGGGA CAGGAAGTAC 
ATTGTTCTGG AGGGGTCAAA AGTCCTCATC TATGACAATG AAGCCAGAGA AGCTGGACAG 
AGGCCGGTGG AAGAATTTGA GCTGTGCCTT CCTGACGGGG ACGTATCTAT TCATGGTGCC 
GTTGGTGCTT CAGAACTTGC AAATACCGCC AAAGCAGATG TCCCATACAT CCTGAAGATG 
GAGTCTCACC CACACACCAC CTGCTGGCCT GGGAGAACCC TCTACTTGCT AGCACCCAGC 
TTCCCCGACA AGCAGCGCTG GGTCACCGCC TTAGAATCTG TCGTCGCAGG TGGGAGAGTT 
TCTAGGGAAA AGGCCGAAGC CGATGCTGCA CGTGACCATA CTTCGAGTGA GCATCAGCCA 
GTGTGGGTTG AGAAATTACT TGGAAACTCT CTGCTGAAAC TGGAGGGCGA TGACCGGCTT 
GACATGAACT GCACCCTGCC CTTCAGTGAC CAGGTAGTGC TGGTGGGCAC GGAGGAAGGC 
CTCTACGCCC TGAATGTCTT GAAAAACTCC TTAACCCACA TCCCAGGGAT TGGCGCAGTC 
TTCCAAATTT ACATCATCAA GGACCTGGAG AAGCTGCTCA TGATAGCAGG GGAAGAGCGG 
GCTCTGTGCC TGGTGGACGT GAAGAAGGTG AAGCAGTCCC TGGCGCAGTC ACACCTGCCT 
GCCCAGCCCG ACGTCTCCCC CAACATATTC GAAGCCGTCA AAGGCTGCCA CTTGTTCGCT 
GCTGGCAAGA TCGAGAACAG CCTGTGCATC TGCGCCGCTA TGCCAAGCAA AGTCGTCATC 
CTCCGCTACA ATGACAACCT CAGCAAGTAC TGCATCCGCA AGGAGATCGA GACCTCAGAG 
CCCTGCAGCT GTATCCACTT CACCAACTAC AGCATCCTCA TTGGGACCAA CAAATTCTAT 
GAGATCGACA TGAAGCAGTA CACGCTTGAC GAGTTCCTGG ACAAGAACGA CCATTCCTTG 
GCACCTGCTG TGTTCGCCTC CTCGTCCAAC AGCTTCCCTG TCTCCATTGT GCAGGCGAAC 
AGCGCCGGGC AGCGAGAAGA ATACCTGCTG TGCTTCCACG AATTTGGGGT GTTCGTGGAT 
TCTTACGGAA GACGTAGCCG CACAGATGAT CTCAAGTGGA GTCGCTTACC TCTGGCCTTC 
GCCTACAGAG AACCTTATCT GTTTGTGACT CACTTCAACT CCCTGGAAGT CATTGAGATC 
CAGGCCAGAT CCTCACTGGG GAGCCCTGCC CGAGCATATC TGGAAATTCC AAACCCTCGC 
TACCTGGGCC CCGCGATTTC CTCCGGAGCG ATTTACCTGG CCTCCTCATA CCAGGACAAG 
TTAAGGGTCA TATGCTGCAA AGGAAACCTC GTGAAGGAGT CAGGCACTGA GCAGCACCGG 
GTGCCCTCCA CCTCCCGCAG CAGCCCCAAC AAGCGAGGCC CACCAACATA CAACGAGCAC 
ATCACCAAAC GCGTGGCCTC CAGCCCGGCG CCACCGGAAG GCCCCAGCCA CCCCCGAGAG 
CCAAGCACAC CGCACCGCTA CCGAGACAGA GAGGGTCGGA CAGAGCTGCG CAGGGACAAG 
TCTCCAGGCC GCCCTCTGGA GCGGGAGAAG TCCCCAGGCC GAATGCTCAG CACTAGGAGA 
GAGCGGTCCC CAGGGAGACT GTTTGAAGAC AGCAGCAGGG GCCGGCTGCC TGCAGGAGCA 
GTGAGGACCC CACTGTCCCA GGTTAACAAG GTCTGGGACC AGTCTTCAGT ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006530213.1
CDS	1203..6176
Translation	MEQEMTRLHRRVSEVEAVLSQKEVELKASETQRSLLEQDLATYITECSSLKRSLEQARMEVSQEDDKALQLLHDIREQSRKLQEIKEQEYQAQVEEMRLMMNQLEEDLVSARRRSDLYESELRESRLAAEEFKRKANECQHKLMKVVSHPPRGDSGGTALDDLHKMQGHAGLTSAKDQGKPEVGEYSKLEKINAEQQLKIQELQEKLEKAVKASTEATELLQNIRQAKERAERELEKLHNREDSSEGIKKKLVEAEERRHSLENKVKRLETMERRENRLKDDIQTKSEQIQQMADKILELEEKHREAQVSAQHLEVHLKQKEQHYEEKIKVLDNQIKKDLADKESLENMMQRHEEEAHEKGKILSEQKAMINAMDSKIRSLEQRIVELSEANKLAANSSLFTQRNMKAQEEMISELRQQKFYLETQAGKLEAQNRKLEEQLEKISHQDHSDKSRLLELETRLREVSLEHEEQKLELKRQLTELQLSLQERESQLTALQAARAALESQLRQAKTELEETTAEAEEEIQALTAHRDEIQRKFDALRNSCTVITDLEEQLNQLTEDNAELNNQNFYLSKQLDEASGANDEIVQLRSEVDHLRREITEREMQLTSQKQAQLSAPDLQTMEALKTTCTMLEEQVLDLEALNDELLEKERQWEAWRSVLGDEKSQFECRVRELQRMLDTEKQSRARADQRITESRQVVELAVKEHKAEILALQQALKEQKLKAESLSDKLNDLEKKHAMLEMNARSLQQKLETERELKQRLLEEQAKLQQQMDLQKNHIFRLTQGLQEALDRADLLKTERSDLEYQLENIQVLYSHEKVKMEGTISQQTKLIDFLQAKMDQPAKKKKGLFSRRKEDPALPTQVPLQYNELKLALEKEKARCAELEEALQKTRIELRSAREEAAHRKATDHPHPSTPATARQQIAMSAIVRSPEHQPSAMSLLAPPSSRRKESSTPEEFSRRLKERMHHNIPHRFNVGLNMRATKCAVCLDTVHFGRQASKCLECQVMCHPKCSTCLPATCGLPAEYATHFTEAFCRDKMNSPGLQSKEPGSSLHLEGWMKVPRNNKRGQQGWDRKYIVLEGSKVLIYDNEAREAGQRPVEEFELCLPDGDVSIHGAVGASELANTAKADVPYILKMESHPHTTCWPGRTLYLLAPSFPDKQRWVTALESVVAGGRVSREKAEADAARDHTSSEHQPVWVEKLLGNSLLKLEGDDRLDMNCTLPFSDQVVLVGTEEGLYALNVLKNSLTHIPGIGAVFQIYIIKDLEKLLMIAGEERALCLVDVKKVKQSLAQSHLPAQPDVSPNIFEAVKGCHLFAAGKIENSLCICAAMPSKVVILRYNDNLSKYCIRKEIETSEPCSCIHFTNYSILIGTNKFYEIDMKQYTLDEFLDKNDHSLAPAVFASSSNSFPVSIVQANSAGQREEYLLCFHEFGVFVDSYGRRSRTDDLKWSRLPLAFAYREPYLFVTHFNSLEVIEIQARSSLGSPARAYLEIPNPRYLGPAISSGAIYLASSYQDKLRVICCKGNLVKESGTEQHRVPSTSRSSPNKRGPPTYNEHITKRVASSPAPPEGPSHPREPSTPHRYRDREGRTELRRDKSPGRPLEREKSPGRMLSTRRERSPGRLFEDSSRGRLPAGAVRTPLSQVNKVWDQSSV

Target ORF information:

RefSeq Version	XM_006530150.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus citron (Cit), transcript variant X25, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006530150.4

ORF Insert Sequence:

ATGGAGCAGG AAATGACCCG GTTACATCGC AGAGTGTCAG AGGTGGAGGC TGTGCTTAGT 
CAGAAGGAGG TGGAGCTGAA GGCCTCTGAG ACTCAGAGAT CCCTCCTGGA GCAGGACCTT 
GCTACCTACA TCACAGAATG CAGTAGCTTA AAGCGAAGTT TGGAGCAAGC GCGGATGGAG 
GTGTCCCAGG AGGATGACAA AGCTCTGCAG CTTCTCCACG ACATCCGAGA GCAGAGCCGG 
AAGCTCCAGG AGATCAAGGA GCAGGAGTAC CAGGCTCAGG TGGAGGAGAT GAGGCTGATG 
ATGAATCAGC TGGAAGAAGA CCTGGTGTCA GCCCGCAGAC GCAGCGATCT CTACGAGTCT 
GAGCTGAGGG AGTCTCGGCT TGCCGCCGAG GAATTCAAGC GGAAGGCAAA CGAATGTCAG 
CACAAACTGA TGAAGGTAGT CAGCCACCCT CCAAGAGGGG ACTCCGGGGG CACGGCCCTG 
GATGATCTTC ACAAGATGCA AGGCCACGCT GGCTTAACGA GTGCTAAGGA CCAAGGGAAG 
CCTGAAGTGG GAGAATATTC CAAACTGGAG AAGATCAATG CTGAGCAGCA GCTGAAGATC 
CAGGAGCTCC AGGAGAAGCT GGAGAAGGCT GTAAAAGCCA GCACAGAGGC CACCGAGCTC 
CTGCAGAATA TCCGCCAGGC AAAGGAGCGA GCAGAGCGGG AGCTAGAGAA GCTACACAAC 
CGGGAAGACT CCTCCGAGGG CATCAAAAAG AAGCTGGTGG AAGCCGAGGA ACGCCGCCAC 
TCCCTGGAGA ACAAGGTAAA GAGACTAGAG ACCATGGAGC GTAGAGAGAA CAGACTGAAG 
GATGACATCC AGACAAAGTC CGAACAGATC CAGCAGATGG CTGATAAAAT TCTGGAGCTG 
GAGGAGAAAC ATCGGGAGGC TCAGGTCTCA GCTCAACATC TAGAAGTACA CTTGAAACAG 
AAAGAACAGC ACTACGAGGA AAAGATCAAG GTATTGGACA ATCAGATAAA GAAGGACCTG 
GCCGACAAGG AGAGCCTGGA GAACATGATG CAGAGACACG AGGAGGAGGC CCACGAGAAG 
GGCAAGATCC TCAGCGAGCA GAAGGCGATG ATCAACGCGA TGGATTCCAA GATCCGATCC 
CTGGAGCAGA GGATCGTGGA GCTGTCGGAA GCCAACAAGC TTGCGGCAAA CAGCAGTCTC 
TTCACCCAGA GGAACATGAA GGCCCAGGAA GAGATGATCT CAGAACTCAG GCAGCAGAAA 
TTTTACCTGG AGACGCAGGC CGGAAAGCTG GAGGCCCAGA ACCGAAAGCT GGAAGAGCAA 
CTGGAGAAGA TCAGCCACCA AGATCACAGT GACAAGAGTC GGCTGCTGGA GCTGGAAACA 
AGGCTGAGGG AGGTCAGCCT GGAGCACGAG GAGCAGAAGC TGGAGCTGAA GCGGCAGCTC 
ACGGAGCTGC AGCTGTCCCT GCAGGAGCGC GAGTCCCAGC TGACGGCCCT GCAGGCCGCC 
CGGGCAGCGC TGGAGAGCCA GCTCCGCCAG GCGAAGACAG AGCTGGAGGA GACAACCGCG 
GAAGCGGAGG AGGAGATCCA GGCGCTCACG GCACATCGAG ATGAGATCCA GCGCAAATTC 
GATGCCCTTC GCAACAGCTG CACGGTCATC ACCGACCTGG AGGAGCAGCT GAACCAGCTC 
ACCGAGGACA ACGCCGAGCT CAACAACCAA AACTTCTACC TGTCCAAACA ACTCGATGAG 
GCTTCCGGGG CCAATGACGA GATTGTGCAG CTGCGGAGTG AGGTGGACCA TCTCCGCCGT 
GAGATCACGG AGCGGGAGAT GCAGCTCACC AGCCAGAAGC AAGCACAACT CAGTGCTCCT 
GATTTGCAGA CGATGGAGGC TCTGAAGACG ACATGCACCA TGCTGGAGGA GCAGGTCCTG 
GACCTGGAGG CCCTGAACGA CGAGCTGCTG GAGAAGGAGC GCCAGTGGGA GGCCTGGCGG 
AGCGTCCTCG GCGACGAGAA GTCCCAGTTC GAGTGTCGAG TTCGAGAGCT ACAGAGGATG 
CTGGACACCG AGAAGCAGAG CAGGGCTAGG GCCGATCAGC GGATCACCGA GTCGCGCCAG 
GTGGTGGAGT TGGCGGTGAA GGAACACAAG GCCGAGATTC TTGCTCTGCA GCAGGCTCTC 
AAGGAGCAGA AGCTCAAAGC CGAGAGCCTG TCGGACAAGC TCAACGACCT GGAGAAGAAG 
CACGCCATGC TGGAGATGAA CGCCCGGAGC TTACAGCAGA AACTAGAGAC AGAGCGGGAG 
CTCAAACAGA GGCTTCTGGA GGAGCAAGCC AAATTGCAGC AGCAGATGGA CCTGCAGAAG 
AACCACATCT TCAGACTGAC GCAAGGGCTG CAGGAGGCGC TGGACCGGGC GGATCTGCTG 
AAGACAGAAA GGAGCGACCT GGAGTACCAG CTGGAAAACA TTCAGGTTCT CTACTCTCAC 
GAGAAAGTGA AAATGGAAGG CACAATCTCT CAGCAAACCA AACTCATTGA TTTCCTGCAA 
GCCAAAATGG ACCAGCCTGC TAAAAAGAAA AAGGGTTTAT TTAGTCGACG GAAAGAGGAC 
CCTGCTTTGC CCACACAGGT TCCTCTGCAG TACAATGAGC TGAAGCTAGC CCTGGAAAAG 
GAGAAAGCCC GATGCGCGGA GCTGGAGGAG GCCCTTCAGA AGACCCGCAT CGAACTCCGC 
TCTGCGCGGG AGGAAGCTGC CCACCGCAAA GCCACGGACC ACCCGCACCC GTCTACGCCA 
GCCACTGCGA GGCAGCAGAT CGCCATGTCT GCCATTGTGC GGTCGCCCGA GCACCAGCCC 
AGTGCCATGA GCCTGCTTGC CCCACCATCC AGCCGCAGAA AGGAGTCGTC AACTCCAGAG 
GAATTCAGCC GGCGTCTGAA AGAGCGCATG CACCACAACA TCCCTCACCG GTTTAATGTG 
GGCCTGAACA TGAGAGCCAC CAAGTGCGCC GTGTGTCTGG ATACTGTGCA CTTTGGACGC 
CAGGCATCCA AATGCCTCGA ATGTCAAGTG ATGTGTCACC CCAAATGCTC CACCTGCTTG 
CCCGCTACCT GTGGCCTGCC AGCTGAATAT GCCACACACT TCACTGAGGC CTTCTGCCGG 
GACAAAATGA ACTCCCCGGG GCTCCAGAGC AAGGAGCCTG GCAGCAGCTT GCACCTGGAA 
GGGTGGATGA AAGTGCCCAG AAATAACAAA CGGGGACAGC AAGGCTGGGA CAGGAAGTAC 
ATTGTTCTGG AGGGGTCAAA AGTCCTCATC TATGACAATG AAGCCAGAGA AGCTGGACAG 
AGGCCGGTGG AAGAATTTGA GCTGTGCCTT CCTGACGGGG ACGTATCTAT TCATGGTGCC 
GTTGGTGCTT CAGAACTTGC AAATACCGCC AAAGCAGATG TCCCATACAT CCTGAAGATG 
GAGTCTCACC CACACACCAC CTGCTGGCCT GGGAGAACCC TCTACTTGCT AGCACCCAGC 
TTCCCCGACA AGCAGCGCTG GGTCACCGCC TTAGAATCTG TCGTCGCAGG TGGGAGAGTT 
TCTAGGGAAA AGGCCGAAGC CGATGCTGCA CGTGACCATA CTTCGAGTGA GCATCAGCCA 
GTGTGGGTTG AGAAATTACT TGGAAACTCT CTGCTGAAAC TGGAGGGCGA TGACCGGCTT 
GACATGAACT GCACCCTGCC CTTCAGTGAC CAGGTAGTGC TGGTGGGCAC GGAGGAAGGC 
CTCTACGCCC TGAATGTCTT GAAAAACTCC TTAACCCACA TCCCAGGGAT TGGCGCAGTC 
TTCCAAATTT ACATCATCAA GGACCTGGAG AAGCTGCTCA TGATAGCAGG GGAAGAGCGG 
GCTCTGTGCC TGGTGGACGT GAAGAAGGTG AAGCAGTCCC TGGCGCAGTC ACACCTGCCT 
GCCCAGCCCG ACGTCTCCCC CAACATATTC GAAGCCGTCA AAGGCTGCCA CTTGTTCGCT 
GCTGGCAAGA TCGAGAACAG CCTGTGCATC TGCGCCGCTA TGCCAAGCAA AGTCGTCATC 
CTCCGCTACA ATGACAACCT CAGCAAGTAC TGCATCCGCA AGGAGATCGA GACCTCAGAG 
CCCTGCAGCT GTATCCACTT CACCAACTAC AGCATCCTCA TTGGGACCAA CAAATTCTAT 
GAGATCGACA TGAAGCAGTA CACGCTTGAC GAGTTCCTGG ACAAGAACGA CCATTCCTTG 
GCACCTGCTG TGTTCGCCTC CTCGTCCAAC AGCTTCCCTG TCTCCATTGT GCAGGCGAAC 
AGCGCCGGGC AGCGAGAAGA ATACCTGCTG TGCTTCCACG AATTTGGGGT GTTCGTGGAT 
TCTTACGGAA GACGTAGCCG CACAGATGAT CTCAAGTGGA GTCGCTTACC TCTGGCCTTC 
GCCTACAGAG AACCTTATCT GTTTGTGACT CACTTCAACT CCCTGGAAGT CATTGAGATC 
CAGGCCAGAT CCTCACTGGG GAGCCCTGCC CGAGCATATC TGGAAATTCC AAACCCTCGC 
TACCTGGGCC CCGCGATTTC CTCCGGAGCG ATTTACCTGG CCTCCTCATA CCAGGACAAG 
TTAAGGGTCA TATGCTGCAA AGGAAACCTC GTGAAGGAGT CAGGCACTGA GCAGCACCGG 
GTGCCCTCCA CCTCCCGCAG CAGCCCCAAC AAGCGAGGCC CACCAACATA CAACGAGCAC 
ATCACCAAAC GCGTGGCCTC CAGCCCGGCG CCACCGGAAG GCCCCAGCCA CCCCCGAGAG 
CCAAGCACAC CGCACCGCTA CCGAGACAGA GAGGGTCGGA CAGAGCTGCG CAGGGACAAG 
TCTCCAGGCC GCCCTCTGGA GCGGGAGAAG TCCCCAGGCC GAATGCTCAG CACTAGGAGA 
GAGCGGTCCC CAGGGAGACT GTTTGAAGAC AGCAGCAGGG GCCGGCTGCC TGCAGGAGCA 
GTGAGGACCC CACTGTCCCA GGTTAACAAG GTCTGGGACC AGTCTTCAGT ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cit ( XM_006530151.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006530214.1 Mus musculus citron (Cit), transcript variant X31, mRNA.

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CloneID	OMu35790
Clone ID Related Accession (Same CDS sequence)	XM_006530151.3 , XM_006530151.4
Accession Version	XM_006530151.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4794bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	citron Rho-interacting kinase isoform X26
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000071.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006530151.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTCCTGG GCGAAGAAGC AATGATGGAG CAGGAAATGA CCCGGTTACA TCGCAGAGTG 
TCAGAGGTGG AGGCTGTGCT TAGTCAGAAG GAGGTGGAGC TGAAGGCCTC TGAGACTCAG 
AGATCCCTCC TGGAGCAGGA CCTTGCTACC TACATCACAG AATGCAGTAG CTTAAAGCGA 
AGTTTGGAGC AAGCGCGGAT GGAGGTGTCC CAGGAGGATG ACAAAGCTCT GCAGCTTCTC 
CACGACATCC GAGAGCAGAG CCGGAAGCTC CAGGAGATCA AGGAGCAGGA GTACCAGGCT 
CAGGTGGAGG AGATGAGGCT GATGATGAAT CAGCTGGAAG AAGACCTGGT GTCAGCCCGC 
AGACGCAGCG ATCTCTACGA GTCTGAGCTG AGGGAGTCTC GGCTTGCCGC CGAGGAATTC 
AAGCGGAAGG CAAACGAATG TCAGCACAAA CTGATGAAGG CTAAGGACCA AGGGAAGCCT 
GAAGTGGGAG AATATTCCAA ACTGGAGAAG ATCAATGCTG AGCAGCAGCT GAAGATCCAG 
GAGCTCCAGG AGAAGCTGGA GAAGGCTGTA AAAGCCAGCA CAGAGGCCAC CGAGCTCCTG 
CAGAATATCC GCCAGGCAAA GGAGCGAGCA GAGCGGGAGC TAGAGAAGCT ACACAACCGG 
GAAGACTCCT CCGAGGGCAT CAAAAAGAAG CTGGTGGAAG CCGAGGAACG CCGCCACTCC 
CTGGAGAACA AGGTAAAGAG ACTAGAGACC ATGGAGCGTA GAGAGAACAG ACTGAAGGAT 
GACATCCAGA CAAAGTCCGA ACAGATCCAG CAGATGGCTG ATAAAATTCT GGAGCTGGAG 
GAGAAACATC GGGAGGCTCA GGTCTCAGCT CAACATCTAG AAGTACACTT GAAACAGAAA 
GAACAGCACT ACGAGGAAAA GATCAAGGTA TTGGACAATC AGATAAAGAA GGACCTGGCC 
GACAAGGAGA GCCTGGAGAA CATGATGCAG AGACACGAGG AGGAGGCCCA CGAGAAGGGC 
AAGATCCTCA GCGAGCAGAA GGCGATGATC AACGCGATGG ATTCCAAGAT CCGATCCCTG 
GAGCAGAGGA TCGTGGAGCT GTCGGAAGCC AACAAGCTTG CGGCAAACAG CAGTCTCTTC 
ACCCAGAGGA ACATGAAGGC CCAGGAAGAG ATGATCTCAG AACTCAGGCA GCAGAAATTT 
TACCTGGAGA CGCAGGCCGG AAAGCTGGAG GCCCAGAACC GAAAGCTGGA AGAGCAACTG 
GAGAAGATCA GCCACCAAGA TCACAGTGAC AAGAGTCGGC TGCTGGAGCT GGAAACAAGG 
CTGAGGGAGG TCAGCCTGGA GCACGAGGAG CAGAAGCTGG AGCTGAAGCG GCAGCTCACG 
GAGCTGCAGC TGTCCCTGCA GGAGCGCGAG TCCCAGCTGA CGGCCCTGCA GGCCGCCCGG 
GCAGCGCTGG AGAGCCAGCT CCGCCAGGCG AAGACAGAGC TGGAGGAGAC AACCGCGGAA 
GCGGAGGAGG AGATCCAGGC GCTCACGGCA CATCGAGATG AGATCCAGCG CAAATTCGAT 
GCCCTTCGCA ACAGCTGCAC GGTCATCACC GACCTGGAGG AGCAGCTGAA CCAGCTCACC 
GAGGACAACG CCGAGCTCAA CAACCAAAAC TTCTACCTGT CCAAACAACT CGATGAGGCT 
TCCGGGGCCA ATGACGAGAT TGTGCAGCTG CGGAGTGAGG TGGACCATCT CCGCCGTGAG 
ATCACGGAGC GGGAGATGCA GCTCACCAGC CAGAAGCAAA CGATGGAGGC TCTGAAGACG 
ACATGCACCA TGCTGGAGGA GCAGGTCCTG GACCTGGAGG CCCTGAACGA CGAGCTGCTG 
GAGAAGGAGC GCCAGTGGGA GGCCTGGCGG AGCGTCCTCG GCGACGAGAA GTCCCAGTTC 
GAGTGTCGAG TTCGAGAGCT ACAGAGGATG CTGGACACCG AGAAGCAGAG CAGGGCTAGG 
GCCGATCAGC GGATCACCGA GTCGCGCCAG GTGGTGGAGT TGGCGGTGAA GGAACACAAG 
GCCGAGATTC TTGCTCTGCA GCAGGCTCTC AAGGAGCAGA AGCTCAAAGC CGAGAGCCTG 
TCGGACAAGC TCAACGACCT GGAGAAGAAG CACGCCATGC TGGAGATGAA CGCCCGGAGC 
TTACAGCAGA AACTAGAGAC AGAGCGGGAG CTCAAACAGA GGCTTCTGGA GGAGCAAGCC 
AAATTGCAGC AGCAGATGGA CCTGCAGAAG AACCACATCT TCAGACTGAC GCAAGGGCTG 
CAGGAGGCGC TGGACCGGGC GGATCTGCTG AAGACAGAAA GGAGCGACCT GGAGTACCAG 
CTGGAAAACA TTCAGGTTCT CTACTCTCAC GAGAAAGTGA AAATGGAAGG CACAATCTCT 
CAGCAAACCA AACTCATTGA TTTCCTGCAA GCCAAAATGG ACCAGCCTGC TAAAAAGAAA 
AAGGTTCCTC TGCAGTACAA TGAGCTGAAG CTAGCCCTGG AAAAGGAGAA AGCCCGATGC 
GCGGAGCTGG AGGAGGCCCT TCAGAAGACC CGCATCGAAC TCCGCTCTGC GCGGGAGGAA 
GCTGCCCACC GCAAAGCCAC GGACCACCCG CACCCGTCTA CGCCAGCCAC TGCGAGGCAG 
CAGATCGCCA TGTCTGCCAT TGTGCGGTCG CCCGAGCACC AGCCCAGTGC CATGAGCCTG 
CTTGCCCCAC CATCCAGCCG CAGAAAGGAG TCGTCAACTC CAGAGGAATT CAGCCGGCGT 
CTGAAAGAGC GCATGCACCA CAACATCCCT CACCGGTTTA ATGTGGGCCT GAACATGAGA 
GCCACCAAGT GCGCCGTGTG TCTGGATACT GTGCACTTTG GACGCCAGGC ATCCAAATGC 
CTCGAATGTC AAGTGATGTG TCACCCCAAA TGCTCCACCT GCTTGCCCGC TACCTGTGGC 
CTGCCAGCTG AATATGCCAC ACACTTCACT GAGGCCTTCT GCCGGGACAA AATGAACTCC 
CCGGGGCTCC AGAGCAAGGA GCCTGGCAGC AGCTTGCACC TGGAAGGGTG GATGAAAGTG 
CCCAGAAATA ACAAACGGGG ACAGCAAGGC TGGGACAGGA AGTACATTGT TCTGGAGGGG 
TCAAAAGTCC TCATCTATGA CAATGAAGCC AGAGAAGCTG GACAGAGGCC GGTGGAAGAA 
TTTGAGCTGT GCCTTCCTGA CGGGGACGTA TCTATTCATG GTGCCGTTGG TGCTTCAGAA 
CTTGCAAATA CCGCCAAAGC AGATGTCCCA TACATCCTGA AGATGGAGTC TCACCCACAC 
ACCACCTGCT GGCCTGGGAG AACCCTCTAC TTGCTAGCAC CCAGCTTCCC CGACAAGCAG 
CGCTGGGTCA CCGCCTTAGA ATCTGTCGTC GCAGGTGGGA GAGTTTCTAG GGAAAAGGCC 
GAAGCCGATG CTAAATTACT TGGAAACTCT CTGCTGAAAC TGGAGGGCGA TGACCGGCTT 
GACATGAACT GCACCCTGCC CTTCAGTGAC CAGGTAGTGC TGGTGGGCAC GGAGGAAGGC 
CTCTACGCCC TGAATGTCTT GAAAAACTCC TTAACCCACA TCCCAGGGAT TGGCGCAGTC 
TTCCAAATTT ACATCATCAA GGACCTGGAG AAGCTGCTCA TGATAGCAGG GGAAGAGCGG 
GCTCTGTGCC TGGTGGACGT GAAGAAGGTG AAGCAGTCCC TGGCGCAGTC ACACCTGCCT 
GCCCAGCCCG ACGTCTCCCC CAACATATTC GAAGCCGTCA AAGGCTGCCA CTTGTTCGCT 
GCTGGCAAGA TCGAGAACAG CCTGTGCATC TGCGCCGCTA TGCCAAGCAA AGTCGTCATC 
CTCCGCTACA ATGACAACCT CAGCAAGTAC TGCATCCGCA AGGAGATCGA GACCTCAGAG 
CCCTGCAGCT GTATCCACTT CACCAACTAC AGCATCCTCA TTGGGACCAA CAAATTCTAT 
GAGATCGACA TGAAGCAGTA CACGCTTGAC GAGTTCCTGG ACAAGAACGA CCATTCCTTG 
GCACCTGCTG TGTTCGCCTC CTCGTCCAAC AGCTTCCCTG TCTCCATTGT GCAGGCGAAC 
AGCGCCGGGC AGCGAGAAGA ATACCTGCTG TGCTTCCACG AATTTGGGGT GTTCGTGGAT 
TCTTACGGAA GACGTAGCCG CACAGATGAT CTCAAGTGGA GTCGCTTACC TCTGGCCTTC 
GCCTACAGAG AACCTTATCT GTTTGTGACT CACTTCAACT CCCTGGAAGT CATTGAGATC 
CAGGCCAGAT CCTCACTGGG GAGCCCTGCC CGAGCATATC TGGAAATTCC AAACCCTCGC 
TACCTGGGCC CCGCGATTTC CTCCGGAGCG ATTTACCTGG CCTCCTCATA CCAGGACAAG 
TTAAGGGTCA TATGCTGCAA AGGAAACCTC GTGAAGGAGT CAGGCACTGA GCAGCACCGG 
GTGCCCTCCA CCTCCCGCAG CAGCCCCAAC AAGCGAGGCC CACCAACATA CAACGAGCAC 
ATCACCAAAC GCGTGGCCTC CAGCCCGGCG CCACCGGAAG GCCCCAGCCA CCCCCGAGAG 
CCAAGCACAC CGCACCGCTA CCGAGACAGA GAGGGTCGGA CAGAGCTGCG CAGGGACAAG 
TCTCCAGGCC GCCCTCTGGA GCGGGAGAAG TCCCCAGGCC GAATGCTCAG CACTAGGAGA 
GAGCGGTCCC CAGGGAGACT GTTTGAAGAC AGCAGCAGGG GCCGGCTGCC TGCAGGAGCA 
GTGAGGACCC CACTGTCCCA GGTTAACAAG GTCTGGGACC AGTCTTCAGT ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006530214.1
CDS	1147..5940
Translation	MLLGEEAMMEQEMTRLHRRVSEVEAVLSQKEVELKASETQRSLLEQDLATYITECSSLKRSLEQARMEVSQEDDKALQLLHDIREQSRKLQEIKEQEYQAQVEEMRLMMNQLEEDLVSARRRSDLYESELRESRLAAEEFKRKANECQHKLMKAKDQGKPEVGEYSKLEKINAEQQLKIQELQEKLEKAVKASTEATELLQNIRQAKERAERELEKLHNREDSSEGIKKKLVEAEERRHSLENKVKRLETMERRENRLKDDIQTKSEQIQQMADKILELEEKHREAQVSAQHLEVHLKQKEQHYEEKIKVLDNQIKKDLADKESLENMMQRHEEEAHEKGKILSEQKAMINAMDSKIRSLEQRIVELSEANKLAANSSLFTQRNMKAQEEMISELRQQKFYLETQAGKLEAQNRKLEEQLEKISHQDHSDKSRLLELETRLREVSLEHEEQKLELKRQLTELQLSLQERESQLTALQAARAALESQLRQAKTELEETTAEAEEEIQALTAHRDEIQRKFDALRNSCTVITDLEEQLNQLTEDNAELNNQNFYLSKQLDEASGANDEIVQLRSEVDHLRREITEREMQLTSQKQTMEALKTTCTMLEEQVLDLEALNDELLEKERQWEAWRSVLGDEKSQFECRVRELQRMLDTEKQSRARADQRITESRQVVELAVKEHKAEILALQQALKEQKLKAESLSDKLNDLEKKHAMLEMNARSLQQKLETERELKQRLLEEQAKLQQQMDLQKNHIFRLTQGLQEALDRADLLKTERSDLEYQLENIQVLYSHEKVKMEGTISQQTKLIDFLQAKMDQPAKKKKVPLQYNELKLALEKEKARCAELEEALQKTRIELRSAREEAAHRKATDHPHPSTPATARQQIAMSAIVRSPEHQPSAMSLLAPPSSRRKESSTPEEFSRRLKERMHHNIPHRFNVGLNMRATKCAVCLDTVHFGRQASKCLECQVMCHPKCSTCLPATCGLPAEYATHFTEAFCRDKMNSPGLQSKEPGSSLHLEGWMKVPRNNKRGQQGWDRKYIVLEGSKVLIYDNEAREAGQRPVEEFELCLPDGDVSIHGAVGASELANTAKADVPYILKMESHPHTTCWPGRTLYLLAPSFPDKQRWVTALESVVAGGRVSREKAEADAKLLGNSLLKLEGDDRLDMNCTLPFSDQVVLVGTEEGLYALNVLKNSLTHIPGIGAVFQIYIIKDLEKLLMIAGEERALCLVDVKKVKQSLAQSHLPAQPDVSPNIFEAVKGCHLFAAGKIENSLCICAAMPSKVVILRYNDNLSKYCIRKEIETSEPCSCIHFTNYSILIGTNKFYEIDMKQYTLDEFLDKNDHSLAPAVFASSSNSFPVSIVQANSAGQREEYLLCFHEFGVFVDSYGRRSRTDDLKWSRLPLAFAYREPYLFVTHFNSLEVIEIQARSSLGSPARAYLEIPNPRYLGPAISSGAIYLASSYQDKLRVICCKGNLVKESGTEQHRVPSTSRSSPNKRGPPTYNEHITKRVASSPAPPEGPSHPREPSTPHRYRDREGRTELRRDKSPGRPLEREKSPGRMLSTRRERSPGRLFEDSSRGRLPAGAVRTPLSQVNKVWDQSSV

Target ORF information:

RefSeq Version	XM_006530151.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus citron (Cit), transcript variant X31, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006530151.4

ORF Insert Sequence:

ATGCTCCTGG GCGAAGAAGC AATGATGGAG CAGGAAATGA CCCGGTTACA TCGCAGAGTG 
TCAGAGGTGG AGGCTGTGCT TAGTCAGAAG GAGGTGGAGC TGAAGGCCTC TGAGACTCAG 
AGATCCCTCC TGGAGCAGGA CCTTGCTACC TACATCACAG AATGCAGTAG CTTAAAGCGA 
AGTTTGGAGC AAGCGCGGAT GGAGGTGTCC CAGGAGGATG ACAAAGCTCT GCAGCTTCTC 
CACGACATCC GAGAGCAGAG CCGGAAGCTC CAGGAGATCA AGGAGCAGGA GTACCAGGCT 
CAGGTGGAGG AGATGAGGCT GATGATGAAT CAGCTGGAAG AAGACCTGGT GTCAGCCCGC 
AGACGCAGCG ATCTCTACGA GTCTGAGCTG AGGGAGTCTC GGCTTGCCGC CGAGGAATTC 
AAGCGGAAGG CAAACGAATG TCAGCACAAA CTGATGAAGG CTAAGGACCA AGGGAAGCCT 
GAAGTGGGAG AATATTCCAA ACTGGAGAAG ATCAATGCTG AGCAGCAGCT GAAGATCCAG 
GAGCTCCAGG AGAAGCTGGA GAAGGCTGTA AAAGCCAGCA CAGAGGCCAC CGAGCTCCTG 
CAGAATATCC GCCAGGCAAA GGAGCGAGCA GAGCGGGAGC TAGAGAAGCT ACACAACCGG 
GAAGACTCCT CCGAGGGCAT CAAAAAGAAG CTGGTGGAAG CCGAGGAACG CCGCCACTCC 
CTGGAGAACA AGGTAAAGAG ACTAGAGACC ATGGAGCGTA GAGAGAACAG ACTGAAGGAT 
GACATCCAGA CAAAGTCCGA ACAGATCCAG CAGATGGCTG ATAAAATTCT GGAGCTGGAG 
GAGAAACATC GGGAGGCTCA GGTCTCAGCT CAACATCTAG AAGTACACTT GAAACAGAAA 
GAACAGCACT ACGAGGAAAA GATCAAGGTA TTGGACAATC AGATAAAGAA GGACCTGGCC 
GACAAGGAGA GCCTGGAGAA CATGATGCAG AGACACGAGG AGGAGGCCCA CGAGAAGGGC 
AAGATCCTCA GCGAGCAGAA GGCGATGATC AACGCGATGG ATTCCAAGAT CCGATCCCTG 
GAGCAGAGGA TCGTGGAGCT GTCGGAAGCC AACAAGCTTG CGGCAAACAG CAGTCTCTTC 
ACCCAGAGGA ACATGAAGGC CCAGGAAGAG ATGATCTCAG AACTCAGGCA GCAGAAATTT 
TACCTGGAGA CGCAGGCCGG AAAGCTGGAG GCCCAGAACC GAAAGCTGGA AGAGCAACTG 
GAGAAGATCA GCCACCAAGA TCACAGTGAC AAGAGTCGGC TGCTGGAGCT GGAAACAAGG 
CTGAGGGAGG TCAGCCTGGA GCACGAGGAG CAGAAGCTGG AGCTGAAGCG GCAGCTCACG 
GAGCTGCAGC TGTCCCTGCA GGAGCGCGAG TCCCAGCTGA CGGCCCTGCA GGCCGCCCGG 
GCAGCGCTGG AGAGCCAGCT CCGCCAGGCG AAGACAGAGC TGGAGGAGAC AACCGCGGAA 
GCGGAGGAGG AGATCCAGGC GCTCACGGCA CATCGAGATG AGATCCAGCG CAAATTCGAT 
GCCCTTCGCA ACAGCTGCAC GGTCATCACC GACCTGGAGG AGCAGCTGAA CCAGCTCACC 
GAGGACAACG CCGAGCTCAA CAACCAAAAC TTCTACCTGT CCAAACAACT CGATGAGGCT 
TCCGGGGCCA ATGACGAGAT TGTGCAGCTG CGGAGTGAGG TGGACCATCT CCGCCGTGAG 
ATCACGGAGC GGGAGATGCA GCTCACCAGC CAGAAGCAAA CGATGGAGGC TCTGAAGACG 
ACATGCACCA TGCTGGAGGA GCAGGTCCTG GACCTGGAGG CCCTGAACGA CGAGCTGCTG 
GAGAAGGAGC GCCAGTGGGA GGCCTGGCGG AGCGTCCTCG GCGACGAGAA GTCCCAGTTC 
GAGTGTCGAG TTCGAGAGCT ACAGAGGATG CTGGACACCG AGAAGCAGAG CAGGGCTAGG 
GCCGATCAGC GGATCACCGA GTCGCGCCAG GTGGTGGAGT TGGCGGTGAA GGAACACAAG 
GCCGAGATTC TTGCTCTGCA GCAGGCTCTC AAGGAGCAGA AGCTCAAAGC CGAGAGCCTG 
TCGGACAAGC TCAACGACCT GGAGAAGAAG CACGCCATGC TGGAGATGAA CGCCCGGAGC 
TTACAGCAGA AACTAGAGAC AGAGCGGGAG CTCAAACAGA GGCTTCTGGA GGAGCAAGCC 
AAATTGCAGC AGCAGATGGA CCTGCAGAAG AACCACATCT TCAGACTGAC GCAAGGGCTG 
CAGGAGGCGC TGGACCGGGC GGATCTGCTG AAGACAGAAA GGAGCGACCT GGAGTACCAG 
CTGGAAAACA TTCAGGTTCT CTACTCTCAC GAGAAAGTGA AAATGGAAGG CACAATCTCT 
CAGCAAACCA AACTCATTGA TTTCCTGCAA GCCAAAATGG ACCAGCCTGC TAAAAAGAAA 
AAGGTTCCTC TGCAGTACAA TGAGCTGAAG CTAGCCCTGG AAAAGGAGAA AGCCCGATGC 
GCGGAGCTGG AGGAGGCCCT TCAGAAGACC CGCATCGAAC TCCGCTCTGC GCGGGAGGAA 
GCTGCCCACC GCAAAGCCAC GGACCACCCG CACCCGTCTA CGCCAGCCAC TGCGAGGCAG 
CAGATCGCCA TGTCTGCCAT TGTGCGGTCG CCCGAGCACC AGCCCAGTGC CATGAGCCTG 
CTTGCCCCAC CATCCAGCCG CAGAAAGGAG TCGTCAACTC CAGAGGAATT CAGCCGGCGT 
CTGAAAGAGC GCATGCACCA CAACATCCCT CACCGGTTTA ATGTGGGCCT GAACATGAGA 
GCCACCAAGT GCGCCGTGTG TCTGGATACT GTGCACTTTG GACGCCAGGC ATCCAAATGC 
CTCGAATGTC AAGTGATGTG TCACCCCAAA TGCTCCACCT GCTTGCCCGC TACCTGTGGC 
CTGCCAGCTG AATATGCCAC ACACTTCACT GAGGCCTTCT GCCGGGACAA AATGAACTCC 
CCGGGGCTCC AGAGCAAGGA GCCTGGCAGC AGCTTGCACC TGGAAGGGTG GATGAAAGTG 
CCCAGAAATA ACAAACGGGG ACAGCAAGGC TGGGACAGGA AGTACATTGT TCTGGAGGGG 
TCAAAAGTCC TCATCTATGA CAATGAAGCC AGAGAAGCTG GACAGAGGCC GGTGGAAGAA 
TTTGAGCTGT GCCTTCCTGA CGGGGACGTA TCTATTCATG GTGCCGTTGG TGCTTCAGAA 
CTTGCAAATA CCGCCAAAGC AGATGTCCCA TACATCCTGA AGATGGAGTC TCACCCACAC 
ACCACCTGCT GGCCTGGGAG AACCCTCTAC TTGCTAGCAC CCAGCTTCCC CGACAAGCAG 
CGCTGGGTCA CCGCCTTAGA ATCTGTCGTC GCAGGTGGGA GAGTTTCTAG GGAAAAGGCC 
GAAGCCGATG CTAAATTACT TGGAAACTCT CTGCTGAAAC TGGAGGGCGA TGACCGGCTT 
GACATGAACT GCACCCTGCC CTTCAGTGAC CAGGTAGTGC TGGTGGGCAC GGAGGAAGGC 
CTCTACGCCC TGAATGTCTT GAAAAACTCC TTAACCCACA TCCCAGGGAT TGGCGCAGTC 
TTCCAAATTT ACATCATCAA GGACCTGGAG AAGCTGCTCA TGATAGCAGG GGAAGAGCGG 
GCTCTGTGCC TGGTGGACGT GAAGAAGGTG AAGCAGTCCC TGGCGCAGTC ACACCTGCCT 
GCCCAGCCCG ACGTCTCCCC CAACATATTC GAAGCCGTCA AAGGCTGCCA CTTGTTCGCT 
GCTGGCAAGA TCGAGAACAG CCTGTGCATC TGCGCCGCTA TGCCAAGCAA AGTCGTCATC 
CTCCGCTACA ATGACAACCT CAGCAAGTAC TGCATCCGCA AGGAGATCGA GACCTCAGAG 
CCCTGCAGCT GTATCCACTT CACCAACTAC AGCATCCTCA TTGGGACCAA CAAATTCTAT 
GAGATCGACA TGAAGCAGTA CACGCTTGAC GAGTTCCTGG ACAAGAACGA CCATTCCTTG 
GCACCTGCTG TGTTCGCCTC CTCGTCCAAC AGCTTCCCTG TCTCCATTGT GCAGGCGAAC 
AGCGCCGGGC AGCGAGAAGA ATACCTGCTG TGCTTCCACG AATTTGGGGT GTTCGTGGAT 
TCTTACGGAA GACGTAGCCG CACAGATGAT CTCAAGTGGA GTCGCTTACC TCTGGCCTTC 
GCCTACAGAG AACCTTATCT GTTTGTGACT CACTTCAACT CCCTGGAAGT CATTGAGATC 
CAGGCCAGAT CCTCACTGGG GAGCCCTGCC CGAGCATATC TGGAAATTCC AAACCCTCGC 
TACCTGGGCC CCGCGATTTC CTCCGGAGCG ATTTACCTGG CCTCCTCATA CCAGGACAAG 
TTAAGGGTCA TATGCTGCAA AGGAAACCTC GTGAAGGAGT CAGGCACTGA GCAGCACCGG 
GTGCCCTCCA CCTCCCGCAG CAGCCCCAAC AAGCGAGGCC CACCAACATA CAACGAGCAC 
ATCACCAAAC GCGTGGCCTC CAGCCCGGCG CCACCGGAAG GCCCCAGCCA CCCCCGAGAG 
CCAAGCACAC CGCACCGCTA CCGAGACAGA GAGGGTCGGA CAGAGCTGCG CAGGGACAAG 
TCTCCAGGCC GCCCTCTGGA GCGGGAGAAG TCCCCAGGCC GAATGCTCAG CACTAGGAGA 
GAGCGGTCCC CAGGGAGACT GTTTGAAGAC AGCAGCAGGG GCCGGCTGCC TGCAGGAGCA 
GTGAGGACCC CACTGTCCCA GGTTAACAAG GTCTGGGACC AGTCTTCAGT ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cit ( XM_017320641.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017176130.1 Mus musculus citron (Cit), transcript variant X23, mRNA.

Price & Availability↑

CloneID	OMu81232
Clone ID Related Accession (Same CDS sequence)	XM_017320641.1 , XM_017320641.2
Accession Version	XM_017320641.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4911bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	citron Rho-interacting kinase isoform X19
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000071.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTCCTGG GCGAAGAAGC AATGATGGAG CAGGAAATGA CCCGGTTACA TCGCAGAGTG 
TCAGAGGTGG AGGCTGTGCT TAGTCAGAAG GAGGTGGAGC TGAAGGCCTC TGAGACTCAG 
AGATCCCTCC TGGAGCAGGA CCTTGCTACC TACATCACAG AATGCAGTAG CTTAAAGCGA 
AGTTTGGAGC AAGCGCGGAT GGAGGTGTCC CAGGAGGATG ACAAAGCTCT GCAGCTTCTC 
CACGACATCC GAGAGCAGAG CCGGAAGCTC CAGGAGATCA AGGAGCAGGA GTACCAGGCT 
CAGGTGGAGG AGATGAGGCT GATGATGAAT CAGCTGGAAG AAGACCTGGT GTCAGCCCGC 
AGACGCAGCG ATCTCTACGA GTCTGAGCTG AGGGAGTCTC GGCTTGCCGC CGAGGAATTC 
AAGCGGAAGG CAAACGAATG TCAGCACAAA CTGATGAAGG CTAAGGACCA AGGGAAGCCT 
GAAGTGGGAG AATATTCCAA ACTGGAGAAG ATCAATGCTG AGCAGCAGCT GAAGATCCAG 
GAGCTCCAGG AGAAGCTGGA GAAGGCTGTA AAAGCCAGCA CAGAGGCCAC CGAGCTCCTG 
CAGAATATCC GCCAGGCAAA GGAGCGAGCA GAGCGGGAGC TAGAGAAGCT ACACAACCGG 
GAAGACTCCT CCGAGGGCAT CAAAAAGAAG CTGGTGGAAG CCGAGGAACG CCGCCACTCC 
CTGGAGAACA AGGTAAAGAG ACTAGAGACC ATGGAGCGTA GAGAGAACAG ACTGAAGGAT 
GACATCCAGA CAAAGTCCGA ACAGATCCAG CAGATGGCTG ATAAAATTCT GGAGCTGGAG 
GAGAAACATC GGGAGGCTCA GGTCTCAGCT CAACATCTAG AAGTACACTT GAAACAGAAA 
GAACAGCACT ACGAGGAAAA GATCAAGGTA TTGGACAATC AGATAAAGAA GGACCTGGCC 
GACAAGGAGA GCCTGGAGAA CATGATGCAG AGACACGAGG AGGAGGCCCA CGAGAAGGGC 
AAGATCCTCA GCGAGCAGAA GGCGATGATC AACGCGATGG ATTCCAAGAT CCGATCCCTG 
GAGCAGAGGA TCGTGGAGCT GTCGGAAGCC AACAAGCTTG CGGCAAACAG CAGTCTCTTC 
ACCCAGAGGA ACATGAAGGC CCAGGAAGAG ATGATCTCAG AACTCAGGCA GCAGAAATTT 
TACCTGGAGA CGCAGGCCGG AAAGCTGGAG GCCCAGAACC GAAAGCTGGA AGAGCAACTG 
GAGAAGATCA GCCACCAAGA TCACAGTGAC AAGAGTCGGC TGCTGGAGCT GGAAACAAGG 
CTGAGGGAGG TCAGCCTGGA GCACGAGGAG CAGAAGCTGG AGCTGAAGCG GCAGCTCACG 
GAGCTGCAGC TGTCCCTGCA GGAGCGCGAG TCCCAGCTGA CGGCCCTGCA GGCCGCCCGG 
GCAGCGCTGG AGAGCCAGCT CCGCCAGGCG AAGACAGAGC TGGAGGAGAC AACCGCGGAA 
GCGGAGGAGG AGATCCAGGC GCTCACGGCA CATCGAGATG AGATCCAGCG CAAATTCGAT 
GCCCTTCGCA ACAGCTGCAC GGTCATCACC GACCTGGAGG AGCAGCTGAA CCAGCTCACC 
GAGGACAACG CCGAGCTCAA CAACCAAAAC TTCTACCTGT CCAAACAACT CGATGAGGCT 
TCCGGGGCCA ATGACGAGAT TGTGCAGCTG CGGAGTGAGG TGGACCATCT CCGCCGTGAG 
ATCACGGAGC GGGAGATGCA GCTCACCAGC CAGAAGCAAG CACAACTCAG TGCTCCTGAT 
TTGCAGACGA TGGAGGCTCT GAAGACGACA TGCACCATGC TGGAGGAGCA GGTCCTGGAC 
CTGGAGGCCC TGAACGACGA GCTGCTGGAG AAGGAGCGCC AGTGGGAGGC CTGGCGGAGC 
GTCCTCGGCG ACGAGAAGTC CCAGTTCGAG TGTCGAGTTC GAGAGCTACA GAGGATGCTG 
GACACCGAGA AGCAGAGCAG GGCTAGGGCC GATCAGCGGA TCACCGAGTC GCGCCAGGTG 
GTGGAGTTGG CGGTGAAGGA ACACAAGGCC GAGATTCTTG CTCTGCAGCA GGCTCTCAAG 
GAGCAGAAGC TCAAAGCCGA GAGCCTGTCG GACAAGCTCA ACGACCTGGA GAAGAAGCAC 
GCCATGCTGG AGATGAACGC CCGGAGCTTA CAGCAGAAAC TAGAGACAGA GCGGGAGCTC 
AAACAGAGGC TTCTGGAGGA GCAAGCCAAA TTGCAGCAGC AGATGGACCT GCAGAAGAAC 
CACATCTTCA GACTGACGCA AGGGCTGCAG GAGGCGCTGG ACCGGGCGGA TCTGCTGAAG 
ACAGAAAGGA GCGACCTGGA GTACCAGCTG GAAAACATTC AGGTTCTCTA CTCTCACGAG 
AAAGTGAAAA TGGAAGGCAC AATCTCTCAG CAAACCAAAC TCATTGATTT CCTGCAAGCC 
AAAATGGACC AGCCTGCTAA AAAGAAAAAG GGTTTATTTA GTCGACGGAA AGAGGACCCT 
GCTTTGCCCA CACAGGTTCC TCTGCAGTAC AATGAGCTGA AGCTAGCCCT GGAAAAGGAG 
AAAGCCCGAT GCGCGGAGCT GGAGGAGGCC CTTCAGAAGA CCCGCATCGA ACTCCGCTCT 
GCGCGGGAGG AAGCTGCCCA CCGCAAAGCC ACGGACCACC CGCACCCGTC TACGCCAGCC 
ACTGCGAGGC AGCAGATCGC CATGTCTGCC ATTGTGCGGT CGCCCGAGCA CCAGCCCAGT 
GCCATGAGCC TGCTTGCCCC ACCATCCAGC CGCAGAAAGG AGTCGTCAAC TCCAGAGGAA 
TTCAGCCGGC GTCTGAAAGA GCGCATGCAC CACAACATCC CTCACCGGTT TAATGTGGGC 
CTGAACATGA GAGCCACCAA GTGCGCCGTG TGTCTGGATA CTGTGCACTT TGGACGCCAG 
GCATCCAAAT GCCTCGAATG TCAAGTGATG TGTCACCCCA AATGCTCCAC CTGCTTGCCC 
GCTACCTGTG GCCTGCCAGC TGAATATGCC ACACACTTCA CTGAGGCCTT CTGCCGGGAC 
AAAATGAACT CCCCGGGGCT CCAGAGCAAG GAGCCTGGCA GCAGCTTGCA CCTGGAAGGG 
TGGATGAAAG TGCCCAGAAA TAACAAACGG GGACAGCAAG GCTGGGACAG GAAGTACATT 
GTTCTGGAGG GGTCAAAAGT CCTCATCTAT GACAATGAAG CCAGAGAAGC TGGACAGAGG 
CCGGTGGAAG AATTTGAGCT GTGCCTTCCT GACGGGGACG TATCTATTCA TGGTGCCGTT 
GGTGCTTCAG AACTTGCAAA TACCGCCAAA GCAGATGTCC CATACATCCT GAAGATGGAG 
TCTCACCCAC ACACCACCTG CTGGCCTGGG AGAACCCTCT ACTTGCTAGC ACCCAGCTTC 
CCCGACAAGC AGCGCTGGGT CACCGCCTTA GAATCTGTCG TCGCAGGTGG GAGAGTTTCT 
AGGGAAAAGG CCGAAGCCGA TGCTGCACGT GACCATACTT CGAGTGAGCA TCAGCCAGTG 
TGGGTTGAGA AATTACTTGG AAACTCTCTG CTGAAACTGG AGGGCGATGA CCGGCTTGAC 
ATGAACTGCA CCCTGCCCTT CAGTGACCAG GTAGTGCTGG TGGGCACGGA GGAAGGCCTC 
TACGCCCTGA ATGTCTTGAA AAACTCCTTA ACCCACATCC CAGGGATTGG CGCAGTCTTC 
CAAATTTACA TCATCAAGGA CCTGGAGAAG CTGCTCATGA TAGCAGGGGA AGAGCGGGCT 
CTGTGCCTGG TGGACGTGAA GAAGGTGAAG CAGTCCCTGG CGCAGTCACA CCTGCCTGCC 
CAGCCCGACG TCTCCCCCAA CATATTCGAA GCCGTCAAAG GCTGCCACTT GTTCGCTGCT 
GGCAAGATCG AGAACAGCCT GTGCATCTGC GCCGCTATGC CAAGCAAAGT CGTCATCCTC 
CGCTACAATG ACAACCTCAG CAAGTACTGC ATCCGCAAGG AGATCGAGAC CTCAGAGCCC 
TGCAGCTGTA TCCACTTCAC CAACTACAGC ATCCTCATTG GGACCAACAA ATTCTATGAG 
ATCGACATGA AGCAGTACAC GCTTGACGAG TTCCTGGACA AGAACGACCA TTCCTTGGCA 
CCTGCTGTGT TCGCCTCCTC GTCCAACAGC TTCCCTGTCT CCATTGTGCA GGCGAACAGC 
GCCGGGCAGC GAGAAGAATA CCTGCTGTGC TTCCACGAAT TTGGGGTGTT CGTGGATTCT 
TACGGAAGAC GTAGCCGCAC AGATGATCTC AAGTGGAGTC GCTTACCTCT GGCCTTCGCC 
TACAGAGAAC CTTATCTGTT TGTGACTCAC TTCAACTCCC TGGAAGTCAT TGAGATCCAG 
GCCAGATCCT CACTGGGGAG CCCTGCCCGA GCATATCTGG AAATTCCAAA CCCTCGCTAC 
CTGGGCCCCG CGATTTCCTC CGGAGCGATT TACCTGGCCT CCTCATACCA GGACAAGTTA 
AGGGTCATAT GCTGCAAAGG AAACCTCGTG AAGGAGTCAG GCACTGAGCA GCACCGGGTG 
CCCTCCACCT CCCGCAGCAG CCCCAACAAG CGAGGCCCAC CAACATACAA CGAGCACATC 
ACCAAACGCG TGGCCTCCAG CCCGGCGCCA CCGGAAGGCC CCAGCCACCC CCGAGAGCCA 
AGCACACCGC ACCGCTACCG AGACAGAGAG GGTCGGACAG AGCTGCGCAG GGACAAGTCT 
CCAGGCCGCC CTCTGGAGCG GGAGAAGTCC CCAGGCCGAA TGCTCAGCAC TAGGAGAGAG 
CGGTCCCCAG GGAGACTGTT TGAAGACAGC AGCAGGGGCC GGCTGCCTGC AGGAGCAGTG 
AGGACCCCAC TGTCCCAGGT TAACAAGGTC TGGGACCAGT CTTCAGTATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017176130.1
CDS	1419..6329
Translation	MLLGEEAMMEQEMTRLHRRVSEVEAVLSQKEVELKASETQRSLLEQDLATYITECSSLKRSLEQARMEVSQEDDKALQLLHDIREQSRKLQEIKEQEYQAQVEEMRLMMNQLEEDLVSARRRSDLYESELRESRLAAEEFKRKANECQHKLMKAKDQGKPEVGEYSKLEKINAEQQLKIQELQEKLEKAVKASTEATELLQNIRQAKERAERELEKLHNREDSSEGIKKKLVEAEERRHSLENKVKRLETMERRENRLKDDIQTKSEQIQQMADKILELEEKHREAQVSAQHLEVHLKQKEQHYEEKIKVLDNQIKKDLADKESLENMMQRHEEEAHEKGKILSEQKAMINAMDSKIRSLEQRIVELSEANKLAANSSLFTQRNMKAQEEMISELRQQKFYLETQAGKLEAQNRKLEEQLEKISHQDHSDKSRLLELETRLREVSLEHEEQKLELKRQLTELQLSLQERESQLTALQAARAALESQLRQAKTELEETTAEAEEEIQALTAHRDEIQRKFDALRNSCTVITDLEEQLNQLTEDNAELNNQNFYLSKQLDEASGANDEIVQLRSEVDHLRREITEREMQLTSQKQAQLSAPDLQTMEALKTTCTMLEEQVLDLEALNDELLEKERQWEAWRSVLGDEKSQFECRVRELQRMLDTEKQSRARADQRITESRQVVELAVKEHKAEILALQQALKEQKLKAESLSDKLNDLEKKHAMLEMNARSLQQKLETERELKQRLLEEQAKLQQQMDLQKNHIFRLTQGLQEALDRADLLKTERSDLEYQLENIQVLYSHEKVKMEGTISQQTKLIDFLQAKMDQPAKKKKGLFSRRKEDPALPTQVPLQYNELKLALEKEKARCAELEEALQKTRIELRSAREEAAHRKATDHPHPSTPATARQQIAMSAIVRSPEHQPSAMSLLAPPSSRRKESSTPEEFSRRLKERMHHNIPHRFNVGLNMRATKCAVCLDTVHFGRQASKCLECQVMCHPKCSTCLPATCGLPAEYATHFTEAFCRDKMNSPGLQSKEPGSSLHLEGWMKVPRNNKRGQQGWDRKYIVLEGSKVLIYDNEAREAGQRPVEEFELCLPDGDVSIHGAVGASELANTAKADVPYILKMESHPHTTCWPGRTLYLLAPSFPDKQRWVTALESVVAGGRVSREKAEADAARDHTSSEHQPVWVEKLLGNSLLKLEGDDRLDMNCTLPFSDQVVLVGTEEGLYALNVLKNSLTHIPGIGAVFQIYIIKDLEKLLMIAGEERALCLVDVKKVKQSLAQSHLPAQPDVSPNIFEAVKGCHLFAAGKIENSLCICAAMPSKVVILRYNDNLSKYCIRKEIETSEPCSCIHFTNYSILIGTNKFYEIDMKQYTLDEFLDKNDHSLAPAVFASSSNSFPVSIVQANSAGQREEYLLCFHEFGVFVDSYGRRSRTDDLKWSRLPLAFAYREPYLFVTHFNSLEVIEIQARSSLGSPARAYLEIPNPRYLGPAISSGAIYLASSYQDKLRVICCKGNLVKESGTEQHRVPSTSRSSPNKRGPPTYNEHITKRVASSPAPPEGPSHPREPSTPHRYRDREGRTELRRDKSPGRPLEREKSPGRMLSTRRERSPGRLFEDSSRGRLPAGAVRTPLSQVNKVWDQSSV

Target ORF information:

RefSeq Version	XM_017320641.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus citron (Cit), transcript variant X23, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017320641.1

ORF Insert Sequence:

ATGCTCCTGG GCGAAGAAGC AATGATGGAG CAGGAAATGA CCCGGTTACA TCGCAGAGTG 
TCAGAGGTGG AGGCTGTGCT TAGTCAGAAG GAGGTGGAGC TGAAGGCCTC TGAGACTCAG 
AGATCCCTCC TGGAGCAGGA CCTTGCTACC TACATCACAG AATGCAGTAG CTTAAAGCGA 
AGTTTGGAGC AAGCGCGGAT GGAGGTGTCC CAGGAGGATG ACAAAGCTCT GCAGCTTCTC 
CACGACATCC GAGAGCAGAG CCGGAAGCTC CAGGAGATCA AGGAGCAGGA GTACCAGGCT 
CAGGTGGAGG AGATGAGGCT GATGATGAAT CAGCTGGAAG AAGACCTGGT GTCAGCCCGC 
AGACGCAGCG ATCTCTACGA GTCTGAGCTG AGGGAGTCTC GGCTTGCCGC CGAGGAATTC 
AAGCGGAAGG CAAACGAATG TCAGCACAAA CTGATGAAGG CTAAGGACCA AGGGAAGCCT 
GAAGTGGGAG AATATTCCAA ACTGGAGAAG ATCAATGCTG AGCAGCAGCT GAAGATCCAG 
GAGCTCCAGG AGAAGCTGGA GAAGGCTGTA AAAGCCAGCA CAGAGGCCAC CGAGCTCCTG 
CAGAATATCC GCCAGGCAAA GGAGCGAGCA GAGCGGGAGC TAGAGAAGCT ACACAACCGG 
GAAGACTCCT CCGAGGGCAT CAAAAAGAAG CTGGTGGAAG CCGAGGAACG CCGCCACTCC 
CTGGAGAACA AGGTAAAGAG ACTAGAGACC ATGGAGCGTA GAGAGAACAG ACTGAAGGAT 
GACATCCAGA CAAAGTCCGA ACAGATCCAG CAGATGGCTG ATAAAATTCT GGAGCTGGAG 
GAGAAACATC GGGAGGCTCA GGTCTCAGCT CAACATCTAG AAGTACACTT GAAACAGAAA 
GAACAGCACT ACGAGGAAAA GATCAAGGTA TTGGACAATC AGATAAAGAA GGACCTGGCC 
GACAAGGAGA GCCTGGAGAA CATGATGCAG AGACACGAGG AGGAGGCCCA CGAGAAGGGC 
AAGATCCTCA GCGAGCAGAA GGCGATGATC AACGCGATGG ATTCCAAGAT CCGATCCCTG 
GAGCAGAGGA TCGTGGAGCT GTCGGAAGCC AACAAGCTTG CGGCAAACAG CAGTCTCTTC 
ACCCAGAGGA ACATGAAGGC CCAGGAAGAG ATGATCTCAG AACTCAGGCA GCAGAAATTT 
TACCTGGAGA CGCAGGCCGG AAAGCTGGAG GCCCAGAACC GAAAGCTGGA AGAGCAACTG 
GAGAAGATCA GCCACCAAGA TCACAGTGAC AAGAGTCGGC TGCTGGAGCT GGAAACAAGG 
CTGAGGGAGG TCAGCCTGGA GCACGAGGAG CAGAAGCTGG AGCTGAAGCG GCAGCTCACG 
GAGCTGCAGC TGTCCCTGCA GGAGCGCGAG TCCCAGCTGA CGGCCCTGCA GGCCGCCCGG 
GCAGCGCTGG AGAGCCAGCT CCGCCAGGCG AAGACAGAGC TGGAGGAGAC AACCGCGGAA 
GCGGAGGAGG AGATCCAGGC GCTCACGGCA CATCGAGATG AGATCCAGCG CAAATTCGAT 
GCCCTTCGCA ACAGCTGCAC GGTCATCACC GACCTGGAGG AGCAGCTGAA CCAGCTCACC 
GAGGACAACG CCGAGCTCAA CAACCAAAAC TTCTACCTGT CCAAACAACT CGATGAGGCT 
TCCGGGGCCA ATGACGAGAT TGTGCAGCTG CGGAGTGAGG TGGACCATCT CCGCCGTGAG 
ATCACGGAGC GGGAGATGCA GCTCACCAGC CAGAAGCAAG CACAACTCAG TGCTCCTGAT 
TTGCAGACGA TGGAGGCTCT GAAGACGACA TGCACCATGC TGGAGGAGCA GGTCCTGGAC 
CTGGAGGCCC TGAACGACGA GCTGCTGGAG AAGGAGCGCC AGTGGGAGGC CTGGCGGAGC 
GTCCTCGGCG ACGAGAAGTC CCAGTTCGAG TGTCGAGTTC GAGAGCTACA GAGGATGCTG 
GACACCGAGA AGCAGAGCAG GGCTAGGGCC GATCAGCGGA TCACCGAGTC GCGCCAGGTG 
GTGGAGTTGG CGGTGAAGGA ACACAAGGCC GAGATTCTTG CTCTGCAGCA GGCTCTCAAG 
GAGCAGAAGC TCAAAGCCGA GAGCCTGTCG GACAAGCTCA ACGACCTGGA GAAGAAGCAC 
GCCATGCTGG AGATGAACGC CCGGAGCTTA CAGCAGAAAC TAGAGACAGA GCGGGAGCTC 
AAACAGAGGC TTCTGGAGGA GCAAGCCAAA TTGCAGCAGC AGATGGACCT GCAGAAGAAC 
CACATCTTCA GACTGACGCA AGGGCTGCAG GAGGCGCTGG ACCGGGCGGA TCTGCTGAAG 
ACAGAAAGGA GCGACCTGGA GTACCAGCTG GAAAACATTC AGGTTCTCTA CTCTCACGAG 
AAAGTGAAAA TGGAAGGCAC AATCTCTCAG CAAACCAAAC TCATTGATTT CCTGCAAGCC 
AAAATGGACC AGCCTGCTAA AAAGAAAAAG GGTTTATTTA GTCGACGGAA AGAGGACCCT 
GCTTTGCCCA CACAGGTTCC TCTGCAGTAC AATGAGCTGA AGCTAGCCCT GGAAAAGGAG 
AAAGCCCGAT GCGCGGAGCT GGAGGAGGCC CTTCAGAAGA CCCGCATCGA ACTCCGCTCT 
GCGCGGGAGG AAGCTGCCCA CCGCAAAGCC ACGGACCACC CGCACCCGTC TACGCCAGCC 
ACTGCGAGGC AGCAGATCGC CATGTCTGCC ATTGTGCGGT CGCCCGAGCA CCAGCCCAGT 
GCCATGAGCC TGCTTGCCCC ACCATCCAGC CGCAGAAAGG AGTCGTCAAC TCCAGAGGAA 
TTCAGCCGGC GTCTGAAAGA GCGCATGCAC CACAACATCC CTCACCGGTT TAATGTGGGC 
CTGAACATGA GAGCCACCAA GTGCGCCGTG TGTCTGGATA CTGTGCACTT TGGACGCCAG 
GCATCCAAAT GCCTCGAATG TCAAGTGATG TGTCACCCCA AATGCTCCAC CTGCTTGCCC 
GCTACCTGTG GCCTGCCAGC TGAATATGCC ACACACTTCA CTGAGGCCTT CTGCCGGGAC 
AAAATGAACT CCCCGGGGCT CCAGAGCAAG GAGCCTGGCA GCAGCTTGCA CCTGGAAGGG 
TGGATGAAAG TGCCCAGAAA TAACAAACGG GGACAGCAAG GCTGGGACAG GAAGTACATT 
GTTCTGGAGG GGTCAAAAGT CCTCATCTAT GACAATGAAG CCAGAGAAGC TGGACAGAGG 
CCGGTGGAAG AATTTGAGCT GTGCCTTCCT GACGGGGACG TATCTATTCA TGGTGCCGTT 
GGTGCTTCAG AACTTGCAAA TACCGCCAAA GCAGATGTCC CATACATCCT GAAGATGGAG 
TCTCACCCAC ACACCACCTG CTGGCCTGGG AGAACCCTCT ACTTGCTAGC ACCCAGCTTC 
CCCGACAAGC AGCGCTGGGT CACCGCCTTA GAATCTGTCG TCGCAGGTGG GAGAGTTTCT 
AGGGAAAAGG CCGAAGCCGA TGCTGCACGT GACCATACTT CGAGTGAGCA TCAGCCAGTG 
TGGGTTGAGA AATTACTTGG AAACTCTCTG CTGAAACTGG AGGGCGATGA CCGGCTTGAC 
ATGAACTGCA CCCTGCCCTT CAGTGACCAG GTAGTGCTGG TGGGCACGGA GGAAGGCCTC 
TACGCCCTGA ATGTCTTGAA AAACTCCTTA ACCCACATCC CAGGGATTGG CGCAGTCTTC 
CAAATTTACA TCATCAAGGA CCTGGAGAAG CTGCTCATGA TAGCAGGGGA AGAGCGGGCT 
CTGTGCCTGG TGGACGTGAA GAAGGTGAAG CAGTCCCTGG CGCAGTCACA CCTGCCTGCC 
CAGCCCGACG TCTCCCCCAA CATATTCGAA GCCGTCAAAG GCTGCCACTT GTTCGCTGCT 
GGCAAGATCG AGAACAGCCT GTGCATCTGC GCCGCTATGC CAAGCAAAGT CGTCATCCTC 
CGCTACAATG ACAACCTCAG CAAGTACTGC ATCCGCAAGG AGATCGAGAC CTCAGAGCCC 
TGCAGCTGTA TCCACTTCAC CAACTACAGC ATCCTCATTG GGACCAACAA ATTCTATGAG 
ATCGACATGA AGCAGTACAC GCTTGACGAG TTCCTGGACA AGAACGACCA TTCCTTGGCA 
CCTGCTGTGT TCGCCTCCTC GTCCAACAGC TTCCCTGTCT CCATTGTGCA GGCGAACAGC 
GCCGGGCAGC GAGAAGAATA CCTGCTGTGC TTCCACGAAT TTGGGGTGTT CGTGGATTCT 
TACGGAAGAC GTAGCCGCAC AGATGATCTC AAGTGGAGTC GCTTACCTCT GGCCTTCGCC 
TACAGAGAAC CTTATCTGTT TGTGACTCAC TTCAACTCCC TGGAAGTCAT TGAGATCCAG 
GCCAGATCCT CACTGGGGAG CCCTGCCCGA GCATATCTGG AAATTCCAAA CCCTCGCTAC 
CTGGGCCCCG CGATTTCCTC CGGAGCGATT TACCTGGCCT CCTCATACCA GGACAAGTTA 
AGGGTCATAT GCTGCAAAGG AAACCTCGTG AAGGAGTCAG GCACTGAGCA GCACCGGGTG 
CCCTCCACCT CCCGCAGCAG CCCCAACAAG CGAGGCCCAC CAACATACAA CGAGCACATC 
ACCAAACGCG TGGCCTCCAG CCCGGCGCCA CCGGAAGGCC CCAGCCACCC CCGAGAGCCA 
AGCACACCGC ACCGCTACCG AGACAGAGAG GGTCGGACAG AGCTGCGCAG GGACAAGTCT 
CCAGGCCGCC CTCTGGAGCG GGAGAAGTCC CCAGGCCGAA TGCTCAGCAC TAGGAGAGAG 
CGGTCCCCAG GGAGACTGTT TGAAGACAGC AGCAGGGGCC GGCTGCCTGC AGGAGCAGTG 
AGGACCCCAC TGTCCCAGGT TAACAAGGTC TGGGACCAGT CTTCAGTATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cit ( XM_017320641.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017176130.1 Mus musculus citron (Cit), transcript variant X26, mRNA.

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CloneID	OMu81232
Clone ID Related Accession (Same CDS sequence)	XM_017320641.1 , XM_017320641.2
Accession Version	XM_017320641.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4911bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	citron Rho-interacting kinase isoform X21
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000071.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_017320641.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTCCTGG GCGAAGAAGC AATGATGGAG CAGGAAATGA CCCGGTTACA TCGCAGAGTG 
TCAGAGGTGG AGGCTGTGCT TAGTCAGAAG GAGGTGGAGC TGAAGGCCTC TGAGACTCAG 
AGATCCCTCC TGGAGCAGGA CCTTGCTACC TACATCACAG AATGCAGTAG CTTAAAGCGA 
AGTTTGGAGC AAGCGCGGAT GGAGGTGTCC CAGGAGGATG ACAAAGCTCT GCAGCTTCTC 
CACGACATCC GAGAGCAGAG CCGGAAGCTC CAGGAGATCA AGGAGCAGGA GTACCAGGCT 
CAGGTGGAGG AGATGAGGCT GATGATGAAT CAGCTGGAAG AAGACCTGGT GTCAGCCCGC 
AGACGCAGCG ATCTCTACGA GTCTGAGCTG AGGGAGTCTC GGCTTGCCGC CGAGGAATTC 
AAGCGGAAGG CAAACGAATG TCAGCACAAA CTGATGAAGG CTAAGGACCA AGGGAAGCCT 
GAAGTGGGAG AATATTCCAA ACTGGAGAAG ATCAATGCTG AGCAGCAGCT GAAGATCCAG 
GAGCTCCAGG AGAAGCTGGA GAAGGCTGTA AAAGCCAGCA CAGAGGCCAC CGAGCTCCTG 
CAGAATATCC GCCAGGCAAA GGAGCGAGCA GAGCGGGAGC TAGAGAAGCT ACACAACCGG 
GAAGACTCCT CCGAGGGCAT CAAAAAGAAG CTGGTGGAAG CCGAGGAACG CCGCCACTCC 
CTGGAGAACA AGGTAAAGAG ACTAGAGACC ATGGAGCGTA GAGAGAACAG ACTGAAGGAT 
GACATCCAGA CAAAGTCCGA ACAGATCCAG CAGATGGCTG ATAAAATTCT GGAGCTGGAG 
GAGAAACATC GGGAGGCTCA GGTCTCAGCT CAACATCTAG AAGTACACTT GAAACAGAAA 
GAACAGCACT ACGAGGAAAA GATCAAGGTA TTGGACAATC AGATAAAGAA GGACCTGGCC 
GACAAGGAGA GCCTGGAGAA CATGATGCAG AGACACGAGG AGGAGGCCCA CGAGAAGGGC 
AAGATCCTCA GCGAGCAGAA GGCGATGATC AACGCGATGG ATTCCAAGAT CCGATCCCTG 
GAGCAGAGGA TCGTGGAGCT GTCGGAAGCC AACAAGCTTG CGGCAAACAG CAGTCTCTTC 
ACCCAGAGGA ACATGAAGGC CCAGGAAGAG ATGATCTCAG AACTCAGGCA GCAGAAATTT 
TACCTGGAGA CGCAGGCCGG AAAGCTGGAG GCCCAGAACC GAAAGCTGGA AGAGCAACTG 
GAGAAGATCA GCCACCAAGA TCACAGTGAC AAGAGTCGGC TGCTGGAGCT GGAAACAAGG 
CTGAGGGAGG TCAGCCTGGA GCACGAGGAG CAGAAGCTGG AGCTGAAGCG GCAGCTCACG 
GAGCTGCAGC TGTCCCTGCA GGAGCGCGAG TCCCAGCTGA CGGCCCTGCA GGCCGCCCGG 
GCAGCGCTGG AGAGCCAGCT CCGCCAGGCG AAGACAGAGC TGGAGGAGAC AACCGCGGAA 
GCGGAGGAGG AGATCCAGGC GCTCACGGCA CATCGAGATG AGATCCAGCG CAAATTCGAT 
GCCCTTCGCA ACAGCTGCAC GGTCATCACC GACCTGGAGG AGCAGCTGAA CCAGCTCACC 
GAGGACAACG CCGAGCTCAA CAACCAAAAC TTCTACCTGT CCAAACAACT CGATGAGGCT 
TCCGGGGCCA ATGACGAGAT TGTGCAGCTG CGGAGTGAGG TGGACCATCT CCGCCGTGAG 
ATCACGGAGC GGGAGATGCA GCTCACCAGC CAGAAGCAAG CACAACTCAG TGCTCCTGAT 
TTGCAGACGA TGGAGGCTCT GAAGACGACA TGCACCATGC TGGAGGAGCA GGTCCTGGAC 
CTGGAGGCCC TGAACGACGA GCTGCTGGAG AAGGAGCGCC AGTGGGAGGC CTGGCGGAGC 
GTCCTCGGCG ACGAGAAGTC CCAGTTCGAG TGTCGAGTTC GAGAGCTACA GAGGATGCTG 
GACACCGAGA AGCAGAGCAG GGCTAGGGCC GATCAGCGGA TCACCGAGTC GCGCCAGGTG 
GTGGAGTTGG CGGTGAAGGA ACACAAGGCC GAGATTCTTG CTCTGCAGCA GGCTCTCAAG 
GAGCAGAAGC TCAAAGCCGA GAGCCTGTCG GACAAGCTCA ACGACCTGGA GAAGAAGCAC 
GCCATGCTGG AGATGAACGC CCGGAGCTTA CAGCAGAAAC TAGAGACAGA GCGGGAGCTC 
AAACAGAGGC TTCTGGAGGA GCAAGCCAAA TTGCAGCAGC AGATGGACCT GCAGAAGAAC 
CACATCTTCA GACTGACGCA AGGGCTGCAG GAGGCGCTGG ACCGGGCGGA TCTGCTGAAG 
ACAGAAAGGA GCGACCTGGA GTACCAGCTG GAAAACATTC AGGTTCTCTA CTCTCACGAG 
AAAGTGAAAA TGGAAGGCAC AATCTCTCAG CAAACCAAAC TCATTGATTT CCTGCAAGCC 
AAAATGGACC AGCCTGCTAA AAAGAAAAAG GGTTTATTTA GTCGACGGAA AGAGGACCCT 
GCTTTGCCCA CACAGGTTCC TCTGCAGTAC AATGAGCTGA AGCTAGCCCT GGAAAAGGAG 
AAAGCCCGAT GCGCGGAGCT GGAGGAGGCC CTTCAGAAGA CCCGCATCGA ACTCCGCTCT 
GCGCGGGAGG AAGCTGCCCA CCGCAAAGCC ACGGACCACC CGCACCCGTC TACGCCAGCC 
ACTGCGAGGC AGCAGATCGC CATGTCTGCC ATTGTGCGGT CGCCCGAGCA CCAGCCCAGT 
GCCATGAGCC TGCTTGCCCC ACCATCCAGC CGCAGAAAGG AGTCGTCAAC TCCAGAGGAA 
TTCAGCCGGC GTCTGAAAGA GCGCATGCAC CACAACATCC CTCACCGGTT TAATGTGGGC 
CTGAACATGA GAGCCACCAA GTGCGCCGTG TGTCTGGATA CTGTGCACTT TGGACGCCAG 
GCATCCAAAT GCCTCGAATG TCAAGTGATG TGTCACCCCA AATGCTCCAC CTGCTTGCCC 
GCTACCTGTG GCCTGCCAGC TGAATATGCC ACACACTTCA CTGAGGCCTT CTGCCGGGAC 
AAAATGAACT CCCCGGGGCT CCAGAGCAAG GAGCCTGGCA GCAGCTTGCA CCTGGAAGGG 
TGGATGAAAG TGCCCAGAAA TAACAAACGG GGACAGCAAG GCTGGGACAG GAAGTACATT 
GTTCTGGAGG GGTCAAAAGT CCTCATCTAT GACAATGAAG CCAGAGAAGC TGGACAGAGG 
CCGGTGGAAG AATTTGAGCT GTGCCTTCCT GACGGGGACG TATCTATTCA TGGTGCCGTT 
GGTGCTTCAG AACTTGCAAA TACCGCCAAA GCAGATGTCC CATACATCCT GAAGATGGAG 
TCTCACCCAC ACACCACCTG CTGGCCTGGG AGAACCCTCT ACTTGCTAGC ACCCAGCTTC 
CCCGACAAGC AGCGCTGGGT CACCGCCTTA GAATCTGTCG TCGCAGGTGG GAGAGTTTCT 
AGGGAAAAGG CCGAAGCCGA TGCTGCACGT GACCATACTT CGAGTGAGCA TCAGCCAGTG 
TGGGTTGAGA AATTACTTGG AAACTCTCTG CTGAAACTGG AGGGCGATGA CCGGCTTGAC 
ATGAACTGCA CCCTGCCCTT CAGTGACCAG GTAGTGCTGG TGGGCACGGA GGAAGGCCTC 
TACGCCCTGA ATGTCTTGAA AAACTCCTTA ACCCACATCC CAGGGATTGG CGCAGTCTTC 
CAAATTTACA TCATCAAGGA CCTGGAGAAG CTGCTCATGA TAGCAGGGGA AGAGCGGGCT 
CTGTGCCTGG TGGACGTGAA GAAGGTGAAG CAGTCCCTGG CGCAGTCACA CCTGCCTGCC 
CAGCCCGACG TCTCCCCCAA CATATTCGAA GCCGTCAAAG GCTGCCACTT GTTCGCTGCT 
GGCAAGATCG AGAACAGCCT GTGCATCTGC GCCGCTATGC CAAGCAAAGT CGTCATCCTC 
CGCTACAATG ACAACCTCAG CAAGTACTGC ATCCGCAAGG AGATCGAGAC CTCAGAGCCC 
TGCAGCTGTA TCCACTTCAC CAACTACAGC ATCCTCATTG GGACCAACAA ATTCTATGAG 
ATCGACATGA AGCAGTACAC GCTTGACGAG TTCCTGGACA AGAACGACCA TTCCTTGGCA 
CCTGCTGTGT TCGCCTCCTC GTCCAACAGC TTCCCTGTCT CCATTGTGCA GGCGAACAGC 
GCCGGGCAGC GAGAAGAATA CCTGCTGTGC TTCCACGAAT TTGGGGTGTT CGTGGATTCT 
TACGGAAGAC GTAGCCGCAC AGATGATCTC AAGTGGAGTC GCTTACCTCT GGCCTTCGCC 
TACAGAGAAC CTTATCTGTT TGTGACTCAC TTCAACTCCC TGGAAGTCAT TGAGATCCAG 
GCCAGATCCT CACTGGGGAG CCCTGCCCGA GCATATCTGG AAATTCCAAA CCCTCGCTAC 
CTGGGCCCCG CGATTTCCTC CGGAGCGATT TACCTGGCCT CCTCATACCA GGACAAGTTA 
AGGGTCATAT GCTGCAAAGG AAACCTCGTG AAGGAGTCAG GCACTGAGCA GCACCGGGTG 
CCCTCCACCT CCCGCAGCAG CCCCAACAAG CGAGGCCCAC CAACATACAA CGAGCACATC 
ACCAAACGCG TGGCCTCCAG CCCGGCGCCA CCGGAAGGCC CCAGCCACCC CCGAGAGCCA 
AGCACACCGC ACCGCTACCG AGACAGAGAG GGTCGGACAG AGCTGCGCAG GGACAAGTCT 
CCAGGCCGCC CTCTGGAGCG GGAGAAGTCC CCAGGCCGAA TGCTCAGCAC TAGGAGAGAG 
CGGTCCCCAG GGAGACTGTT TGAAGACAGC AGCAGGGGCC GGCTGCCTGC AGGAGCAGTG 
AGGACCCCAC TGTCCCAGGT TAACAAGGTC TGGGACCAGT CTTCAGTATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017176130.1
CDS	1147..6057
Translation	MLLGEEAMMEQEMTRLHRRVSEVEAVLSQKEVELKASETQRSLLEQDLATYITECSSLKRSLEQARMEVSQEDDKALQLLHDIREQSRKLQEIKEQEYQAQVEEMRLMMNQLEEDLVSARRRSDLYESELRESRLAAEEFKRKANECQHKLMKAKDQGKPEVGEYSKLEKINAEQQLKIQELQEKLEKAVKASTEATELLQNIRQAKERAERELEKLHNREDSSEGIKKKLVEAEERRHSLENKVKRLETMERRENRLKDDIQTKSEQIQQMADKILELEEKHREAQVSAQHLEVHLKQKEQHYEEKIKVLDNQIKKDLADKESLENMMQRHEEEAHEKGKILSEQKAMINAMDSKIRSLEQRIVELSEANKLAANSSLFTQRNMKAQEEMISELRQQKFYLETQAGKLEAQNRKLEEQLEKISHQDHSDKSRLLELETRLREVSLEHEEQKLELKRQLTELQLSLQERESQLTALQAARAALESQLRQAKTELEETTAEAEEEIQALTAHRDEIQRKFDALRNSCTVITDLEEQLNQLTEDNAELNNQNFYLSKQLDEASGANDEIVQLRSEVDHLRREITEREMQLTSQKQAQLSAPDLQTMEALKTTCTMLEEQVLDLEALNDELLEKERQWEAWRSVLGDEKSQFECRVRELQRMLDTEKQSRARADQRITESRQVVELAVKEHKAEILALQQALKEQKLKAESLSDKLNDLEKKHAMLEMNARSLQQKLETERELKQRLLEEQAKLQQQMDLQKNHIFRLTQGLQEALDRADLLKTERSDLEYQLENIQVLYSHEKVKMEGTISQQTKLIDFLQAKMDQPAKKKKGLFSRRKEDPALPTQVPLQYNELKLALEKEKARCAELEEALQKTRIELRSAREEAAHRKATDHPHPSTPATARQQIAMSAIVRSPEHQPSAMSLLAPPSSRRKESSTPEEFSRRLKERMHHNIPHRFNVGLNMRATKCAVCLDTVHFGRQASKCLECQVMCHPKCSTCLPATCGLPAEYATHFTEAFCRDKMNSPGLQSKEPGSSLHLEGWMKVPRNNKRGQQGWDRKYIVLEGSKVLIYDNEAREAGQRPVEEFELCLPDGDVSIHGAVGASELANTAKADVPYILKMESHPHTTCWPGRTLYLLAPSFPDKQRWVTALESVVAGGRVSREKAEADAARDHTSSEHQPVWVEKLLGNSLLKLEGDDRLDMNCTLPFSDQVVLVGTEEGLYALNVLKNSLTHIPGIGAVFQIYIIKDLEKLLMIAGEERALCLVDVKKVKQSLAQSHLPAQPDVSPNIFEAVKGCHLFAAGKIENSLCICAAMPSKVVILRYNDNLSKYCIRKEIETSEPCSCIHFTNYSILIGTNKFYEIDMKQYTLDEFLDKNDHSLAPAVFASSSNSFPVSIVQANSAGQREEYLLCFHEFGVFVDSYGRRSRTDDLKWSRLPLAFAYREPYLFVTHFNSLEVIEIQARSSLGSPARAYLEIPNPRYLGPAISSGAIYLASSYQDKLRVICCKGNLVKESGTEQHRVPSTSRSSPNKRGPPTYNEHITKRVASSPAPPEGPSHPREPSTPHRYRDREGRTELRRDKSPGRPLEREKSPGRMLSTRRERSPGRLFEDSSRGRLPAGAVRTPLSQVNKVWDQSSV

Target ORF information:

RefSeq Version	XM_017320641.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus citron (Cit), transcript variant X26, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017320641.2

ORF Insert Sequence:

ATGCTCCTGG GCGAAGAAGC AATGATGGAG CAGGAAATGA CCCGGTTACA TCGCAGAGTG 
TCAGAGGTGG AGGCTGTGCT TAGTCAGAAG GAGGTGGAGC TGAAGGCCTC TGAGACTCAG 
AGATCCCTCC TGGAGCAGGA CCTTGCTACC TACATCACAG AATGCAGTAG CTTAAAGCGA 
AGTTTGGAGC AAGCGCGGAT GGAGGTGTCC CAGGAGGATG ACAAAGCTCT GCAGCTTCTC 
CACGACATCC GAGAGCAGAG CCGGAAGCTC CAGGAGATCA AGGAGCAGGA GTACCAGGCT 
CAGGTGGAGG AGATGAGGCT GATGATGAAT CAGCTGGAAG AAGACCTGGT GTCAGCCCGC 
AGACGCAGCG ATCTCTACGA GTCTGAGCTG AGGGAGTCTC GGCTTGCCGC CGAGGAATTC 
AAGCGGAAGG CAAACGAATG TCAGCACAAA CTGATGAAGG CTAAGGACCA AGGGAAGCCT 
GAAGTGGGAG AATATTCCAA ACTGGAGAAG ATCAATGCTG AGCAGCAGCT GAAGATCCAG 
GAGCTCCAGG AGAAGCTGGA GAAGGCTGTA AAAGCCAGCA CAGAGGCCAC CGAGCTCCTG 
CAGAATATCC GCCAGGCAAA GGAGCGAGCA GAGCGGGAGC TAGAGAAGCT ACACAACCGG 
GAAGACTCCT CCGAGGGCAT CAAAAAGAAG CTGGTGGAAG CCGAGGAACG CCGCCACTCC 
CTGGAGAACA AGGTAAAGAG ACTAGAGACC ATGGAGCGTA GAGAGAACAG ACTGAAGGAT 
GACATCCAGA CAAAGTCCGA ACAGATCCAG CAGATGGCTG ATAAAATTCT GGAGCTGGAG 
GAGAAACATC GGGAGGCTCA GGTCTCAGCT CAACATCTAG AAGTACACTT GAAACAGAAA 
GAACAGCACT ACGAGGAAAA GATCAAGGTA TTGGACAATC AGATAAAGAA GGACCTGGCC 
GACAAGGAGA GCCTGGAGAA CATGATGCAG AGACACGAGG AGGAGGCCCA CGAGAAGGGC 
AAGATCCTCA GCGAGCAGAA GGCGATGATC AACGCGATGG ATTCCAAGAT CCGATCCCTG 
GAGCAGAGGA TCGTGGAGCT GTCGGAAGCC AACAAGCTTG CGGCAAACAG CAGTCTCTTC 
ACCCAGAGGA ACATGAAGGC CCAGGAAGAG ATGATCTCAG AACTCAGGCA GCAGAAATTT 
TACCTGGAGA CGCAGGCCGG AAAGCTGGAG GCCCAGAACC GAAAGCTGGA AGAGCAACTG 
GAGAAGATCA GCCACCAAGA TCACAGTGAC AAGAGTCGGC TGCTGGAGCT GGAAACAAGG 
CTGAGGGAGG TCAGCCTGGA GCACGAGGAG CAGAAGCTGG AGCTGAAGCG GCAGCTCACG 
GAGCTGCAGC TGTCCCTGCA GGAGCGCGAG TCCCAGCTGA CGGCCCTGCA GGCCGCCCGG 
GCAGCGCTGG AGAGCCAGCT CCGCCAGGCG AAGACAGAGC TGGAGGAGAC AACCGCGGAA 
GCGGAGGAGG AGATCCAGGC GCTCACGGCA CATCGAGATG AGATCCAGCG CAAATTCGAT 
GCCCTTCGCA ACAGCTGCAC GGTCATCACC GACCTGGAGG AGCAGCTGAA CCAGCTCACC 
GAGGACAACG CCGAGCTCAA CAACCAAAAC TTCTACCTGT CCAAACAACT CGATGAGGCT 
TCCGGGGCCA ATGACGAGAT TGTGCAGCTG CGGAGTGAGG TGGACCATCT CCGCCGTGAG 
ATCACGGAGC GGGAGATGCA GCTCACCAGC CAGAAGCAAG CACAACTCAG TGCTCCTGAT 
TTGCAGACGA TGGAGGCTCT GAAGACGACA TGCACCATGC TGGAGGAGCA GGTCCTGGAC 
CTGGAGGCCC TGAACGACGA GCTGCTGGAG AAGGAGCGCC AGTGGGAGGC CTGGCGGAGC 
GTCCTCGGCG ACGAGAAGTC CCAGTTCGAG TGTCGAGTTC GAGAGCTACA GAGGATGCTG 
GACACCGAGA AGCAGAGCAG GGCTAGGGCC GATCAGCGGA TCACCGAGTC GCGCCAGGTG 
GTGGAGTTGG CGGTGAAGGA ACACAAGGCC GAGATTCTTG CTCTGCAGCA GGCTCTCAAG 
GAGCAGAAGC TCAAAGCCGA GAGCCTGTCG GACAAGCTCA ACGACCTGGA GAAGAAGCAC 
GCCATGCTGG AGATGAACGC CCGGAGCTTA CAGCAGAAAC TAGAGACAGA GCGGGAGCTC 
AAACAGAGGC TTCTGGAGGA GCAAGCCAAA TTGCAGCAGC AGATGGACCT GCAGAAGAAC 
CACATCTTCA GACTGACGCA AGGGCTGCAG GAGGCGCTGG ACCGGGCGGA TCTGCTGAAG 
ACAGAAAGGA GCGACCTGGA GTACCAGCTG GAAAACATTC AGGTTCTCTA CTCTCACGAG 
AAAGTGAAAA TGGAAGGCAC AATCTCTCAG CAAACCAAAC TCATTGATTT CCTGCAAGCC 
AAAATGGACC AGCCTGCTAA AAAGAAAAAG GGTTTATTTA GTCGACGGAA AGAGGACCCT 
GCTTTGCCCA CACAGGTTCC TCTGCAGTAC AATGAGCTGA AGCTAGCCCT GGAAAAGGAG 
AAAGCCCGAT GCGCGGAGCT GGAGGAGGCC CTTCAGAAGA CCCGCATCGA ACTCCGCTCT 
GCGCGGGAGG AAGCTGCCCA CCGCAAAGCC ACGGACCACC CGCACCCGTC TACGCCAGCC 
ACTGCGAGGC AGCAGATCGC CATGTCTGCC ATTGTGCGGT CGCCCGAGCA CCAGCCCAGT 
GCCATGAGCC TGCTTGCCCC ACCATCCAGC CGCAGAAAGG AGTCGTCAAC TCCAGAGGAA 
TTCAGCCGGC GTCTGAAAGA GCGCATGCAC CACAACATCC CTCACCGGTT TAATGTGGGC 
CTGAACATGA GAGCCACCAA GTGCGCCGTG TGTCTGGATA CTGTGCACTT TGGACGCCAG 
GCATCCAAAT GCCTCGAATG TCAAGTGATG TGTCACCCCA AATGCTCCAC CTGCTTGCCC 
GCTACCTGTG GCCTGCCAGC TGAATATGCC ACACACTTCA CTGAGGCCTT CTGCCGGGAC 
AAAATGAACT CCCCGGGGCT CCAGAGCAAG GAGCCTGGCA GCAGCTTGCA CCTGGAAGGG 
TGGATGAAAG TGCCCAGAAA TAACAAACGG GGACAGCAAG GCTGGGACAG GAAGTACATT 
GTTCTGGAGG GGTCAAAAGT CCTCATCTAT GACAATGAAG CCAGAGAAGC TGGACAGAGG 
CCGGTGGAAG AATTTGAGCT GTGCCTTCCT GACGGGGACG TATCTATTCA TGGTGCCGTT 
GGTGCTTCAG AACTTGCAAA TACCGCCAAA GCAGATGTCC CATACATCCT GAAGATGGAG 
TCTCACCCAC ACACCACCTG CTGGCCTGGG AGAACCCTCT ACTTGCTAGC ACCCAGCTTC 
CCCGACAAGC AGCGCTGGGT CACCGCCTTA GAATCTGTCG TCGCAGGTGG GAGAGTTTCT 
AGGGAAAAGG CCGAAGCCGA TGCTGCACGT GACCATACTT CGAGTGAGCA TCAGCCAGTG 
TGGGTTGAGA AATTACTTGG AAACTCTCTG CTGAAACTGG AGGGCGATGA CCGGCTTGAC 
ATGAACTGCA CCCTGCCCTT CAGTGACCAG GTAGTGCTGG TGGGCACGGA GGAAGGCCTC 
TACGCCCTGA ATGTCTTGAA AAACTCCTTA ACCCACATCC CAGGGATTGG CGCAGTCTTC 
CAAATTTACA TCATCAAGGA CCTGGAGAAG CTGCTCATGA TAGCAGGGGA AGAGCGGGCT 
CTGTGCCTGG TGGACGTGAA GAAGGTGAAG CAGTCCCTGG CGCAGTCACA CCTGCCTGCC 
CAGCCCGACG TCTCCCCCAA CATATTCGAA GCCGTCAAAG GCTGCCACTT GTTCGCTGCT 
GGCAAGATCG AGAACAGCCT GTGCATCTGC GCCGCTATGC CAAGCAAAGT CGTCATCCTC 
CGCTACAATG ACAACCTCAG CAAGTACTGC ATCCGCAAGG AGATCGAGAC CTCAGAGCCC 
TGCAGCTGTA TCCACTTCAC CAACTACAGC ATCCTCATTG GGACCAACAA ATTCTATGAG 
ATCGACATGA AGCAGTACAC GCTTGACGAG TTCCTGGACA AGAACGACCA TTCCTTGGCA 
CCTGCTGTGT TCGCCTCCTC GTCCAACAGC TTCCCTGTCT CCATTGTGCA GGCGAACAGC 
GCCGGGCAGC GAGAAGAATA CCTGCTGTGC TTCCACGAAT TTGGGGTGTT CGTGGATTCT 
TACGGAAGAC GTAGCCGCAC AGATGATCTC AAGTGGAGTC GCTTACCTCT GGCCTTCGCC 
TACAGAGAAC CTTATCTGTT TGTGACTCAC TTCAACTCCC TGGAAGTCAT TGAGATCCAG 
GCCAGATCCT CACTGGGGAG CCCTGCCCGA GCATATCTGG AAATTCCAAA CCCTCGCTAC 
CTGGGCCCCG CGATTTCCTC CGGAGCGATT TACCTGGCCT CCTCATACCA GGACAAGTTA 
AGGGTCATAT GCTGCAAAGG AAACCTCGTG AAGGAGTCAG GCACTGAGCA GCACCGGGTG 
CCCTCCACCT CCCGCAGCAG CCCCAACAAG CGAGGCCCAC CAACATACAA CGAGCACATC 
ACCAAACGCG TGGCCTCCAG CCCGGCGCCA CCGGAAGGCC CCAGCCACCC CCGAGAGCCA 
AGCACACCGC ACCGCTACCG AGACAGAGAG GGTCGGACAG AGCTGCGCAG GGACAAGTCT 
CCAGGCCGCC CTCTGGAGCG GGAGAAGTCC CCAGGCCGAA TGCTCAGCAC TAGGAGAGAG 
CGGTCCCCAG GGAGACTGTT TGAAGACAGC AGCAGGGGCC GGCTGCCTGC AGGAGCAGTG 
AGGACCCCAC TGTCCCAGGT TAACAAGGTC TGGGACCAGT CTTCAGTATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cit ( XM_017320642.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017176131.1 Mus musculus citron (Cit), transcript variant X24, mRNA.

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CloneID	OMu81233
Clone ID Related Accession (Same CDS sequence)	XM_017320642.1
Accession Version	XM_017320642.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4887bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	citron Rho-interacting kinase isoform X20
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000071.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGCAGG AAATGACCCG GTTACATCGC AGAGTGTCAG AGGTGGAGGC TGTGCTTAGT 
CAGAAGGAGG TGGAGCTGAA GGCCTCTGAG ACTCAGAGAT CCCTCCTGGA GCAGGACCTT 
GCTACCTACA TCACAGAATG CAGTAGCTTA AAGCGAAGTT TGGAGCAAGC GCGGATGGAG 
GTGTCCCAGG AGGATGACAA AGCTCTGCAG CTTCTCCACG ACATCCGAGA GCAGAGCCGG 
AAGCTCCAGG AGATCAAGGA GCAGGAGTAC CAGGCTCAGG TGGAGGAGAT GAGGCTGATG 
ATGAATCAGC TGGAAGAAGA CCTGGTGTCA GCCCGCAGAC GCAGCGATCT CTACGAGTCT 
GAGCTGAGGG AGTCTCGGCT TGCCGCCGAG GAATTCAAGC GGAAGGCAAA CGAATGTCAG 
CACAAACTGA TGAAGGCTAA GGACCAAGGG AAGCCTGAAG TGGGAGAATA TTCCAAACTG 
GAGAAGATCA ATGCTGAGCA GCAGCTGAAG ATCCAGGAGC TCCAGGAGAA GCTGGAGAAG 
GCTGTAAAAG CCAGCACAGA GGCCACCGAG CTCCTGCAGA ATATCCGCCA GGCAAAGGAG 
CGAGCAGAGC GGGAGCTAGA GAAGCTACAC AACCGGGAAG ACTCCTCCGA GGGCATCAAA 
AAGAAGCTGG TGGAAGCCGA GGAACGCCGC CACTCCCTGG AGAACAAGGT AAAGAGACTA 
GAGACCATGG AGCGTAGAGA GAACAGACTG AAGGATGACA TCCAGACAAA GTCCGAACAG 
ATCCAGCAGA TGGCTGATAA AATTCTGGAG CTGGAGGAGA AACATCGGGA GGCTCAGGTC 
TCAGCTCAAC ATCTAGAAGT ACACTTGAAA CAGAAAGAAC AGCACTACGA GGAAAAGATC 
AAGGTATTGG ACAATCAGAT AAAGAAGGAC CTGGCCGACA AGGAGAGCCT GGAGAACATG 
ATGCAGAGAC ACGAGGAGGA GGCCCACGAG AAGGGCAAGA TCCTCAGCGA GCAGAAGGCG 
ATGATCAACG CGATGGATTC CAAGATCCGA TCCCTGGAGC AGAGGATCGT GGAGCTGTCG 
GAAGCCAACA AGCTTGCGGC AAACAGCAGT CTCTTCACCC AGAGGAACAT GAAGGCCCAG 
GAAGAGATGA TCTCAGAACT CAGGCAGCAG AAATTTTACC TGGAGACGCA GGCCGGAAAG 
CTGGAGGCCC AGAACCGAAA GCTGGAAGAG CAACTGGAGA AGATCAGCCA CCAAGATCAC 
AGTGACAAGA GTCGGCTGCT GGAGCTGGAA ACAAGGCTGA GGGAGGTCAG CCTGGAGCAC 
GAGGAGCAGA AGCTGGAGCT GAAGCGGCAG CTCACGGAGC TGCAGCTGTC CCTGCAGGAG 
CGCGAGTCCC AGCTGACGGC CCTGCAGGCC GCCCGGGCAG CGCTGGAGAG CCAGCTCCGC 
CAGGCGAAGA CAGAGCTGGA GGAGACAACC GCGGAAGCGG AGGAGGAGAT CCAGGCGCTC 
ACGGCACATC GAGATGAGAT CCAGCGCAAA TTCGATGCCC TTCGCAACAG CTGCACGGTC 
ATCACCGACC TGGAGGAGCA GCTGAACCAG CTCACCGAGG ACAACGCCGA GCTCAACAAC 
CAAAACTTCT ACCTGTCCAA ACAACTCGAT GAGGCTTCCG GGGCCAATGA CGAGATTGTG 
CAGCTGCGGA GTGAGGTGGA CCATCTCCGC CGTGAGATCA CGGAGCGGGA GATGCAGCTC 
ACCAGCCAGA AGCAAGCACA ACTCAGTGCT CCTGATTTGC AGACGATGGA GGCTCTGAAG 
ACGACATGCA CCATGCTGGA GGAGCAGGTC CTGGACCTGG AGGCCCTGAA CGACGAGCTG 
CTGGAGAAGG AGCGCCAGTG GGAGGCCTGG CGGAGCGTCC TCGGCGACGA GAAGTCCCAG 
TTCGAGTGTC GAGTTCGAGA GCTACAGAGG ATGCTGGACA CCGAGAAGCA GAGCAGGGCT 
AGGGCCGATC AGCGGATCAC CGAGTCGCGC CAGGTGGTGG AGTTGGCGGT GAAGGAACAC 
AAGGCCGAGA TTCTTGCTCT GCAGCAGGCT CTCAAGGAGC AGAAGCTCAA AGCCGAGAGC 
CTGTCGGACA AGCTCAACGA CCTGGAGAAG AAGCACGCCA TGCTGGAGAT GAACGCCCGG 
AGCTTACAGC AGAAACTAGA GACAGAGCGG GAGCTCAAAC AGAGGCTTCT GGAGGAGCAA 
GCCAAATTGC AGCAGCAGAT GGACCTGCAG AAGAACCACA TCTTCAGACT GACGCAAGGG 
CTGCAGGAGG CGCTGGACCG GGCGGATCTG CTGAAGACAG AAAGGAGCGA CCTGGAGTAC 
CAGCTGGAAA ACATTCAGGT TCTCTACTCT CACGAGAAAG TGAAAATGGA AGGCACAATC 
TCTCAGCAAA CCAAACTCAT TGATTTCCTG CAAGCCAAAA TGGACCAGCC TGCTAAAAAG 
AAAAAGGGTT TATTTAGTCG ACGGAAAGAG GACCCTGCTT TGCCCACACA GGTTCCTCTG 
CAGTACAATG AGCTGAAGCT AGCCCTGGAA AAGGAGAAAG CCCGATGCGC GGAGCTGGAG 
GAGGCCCTTC AGAAGACCCG CATCGAACTC CGCTCTGCGC GGGAGGAAGC TGCCCACCGC 
AAAGCCACGG ACCACCCGCA CCCGTCTACG CCAGCCACTG CGAGGCAGCA GATCGCCATG 
TCTGCCATTG TGCGGTCGCC CGAGCACCAG CCCAGTGCCA TGAGCCTGCT TGCCCCACCA 
TCCAGCCGCA GAAAGGAGTC GTCAACTCCA GAGGAATTCA GCCGGCGTCT GAAAGAGCGC 
ATGCACCACA ACATCCCTCA CCGGTTTAAT GTGGGCCTGA ACATGAGAGC CACCAAGTGC 
GCCGTGTGTC TGGATACTGT GCACTTTGGA CGCCAGGCAT CCAAATGCCT CGAATGTCAA 
GTGATGTGTC ACCCCAAATG CTCCACCTGC TTGCCCGCTA CCTGTGGCCT GCCAGCTGAA 
TATGCCACAC ACTTCACTGA GGCCTTCTGC CGGGACAAAA TGAACTCCCC GGGGCTCCAG 
AGCAAGGAGC CTGGCAGCAG CTTGCACCTG GAAGGGTGGA TGAAAGTGCC CAGAAATAAC 
AAACGGGGAC AGCAAGGCTG GGACAGGAAG TACATTGTTC TGGAGGGGTC AAAAGTCCTC 
ATCTATGACA ATGAAGCCAG AGAAGCTGGA CAGAGGCCGG TGGAAGAATT TGAGCTGTGC 
CTTCCTGACG GGGACGTATC TATTCATGGT GCCGTTGGTG CTTCAGAACT TGCAAATACC 
GCCAAAGCAG ATGTCCCATA CATCCTGAAG ATGGAGTCTC ACCCACACAC CACCTGCTGG 
CCTGGGAGAA CCCTCTACTT GCTAGCACCC AGCTTCCCCG ACAAGCAGCG CTGGGTCACC 
GCCTTAGAAT CTGTCGTCGC AGGTGGGAGA GTTTCTAGGG AAAAGGCCGA AGCCGATGCT 
GCACGTGACC ATACTTCGAG TGAGCATCAG CCAGTGTGGG TTGAGAAATT ACTTGGAAAC 
TCTCTGCTGA AACTGGAGGG CGATGACCGG CTTGACATGA ACTGCACCCT GCCCTTCAGT 
GACCAGGTAG TGCTGGTGGG CACGGAGGAA GGCCTCTACG CCCTGAATGT CTTGAAAAAC 
TCCTTAACCC ACATCCCAGG GATTGGCGCA GTCTTCCAAA TTTACATCAT CAAGGACCTG 
GAGAAGCTGC TCATGATAGC AGGGGAAGAG CGGGCTCTGT GCCTGGTGGA CGTGAAGAAG 
GTGAAGCAGT CCCTGGCGCA GTCACACCTG CCTGCCCAGC CCGACGTCTC CCCCAACATA 
TTCGAAGCCG TCAAAGGCTG CCACTTGTTC GCTGCTGGCA AGATCGAGAA CAGCCTGTGC 
ATCTGCGCCG CTATGCCAAG CAAAGTCGTC ATCCTCCGCT ACAATGACAA CCTCAGCAAG 
TACTGCATCC GCAAGGAGAT CGAGACCTCA GAGCCCTGCA GCTGTATCCA CTTCACCAAC 
TACAGCATCC TCATTGGGAC CAACAAATTC TATGAGATCG ACATGAAGCA GTACACGCTT 
GACGAGTTCC TGGACAAGAA CGACCATTCC TTGGCACCTG CTGTGTTCGC CTCCTCGTCC 
AACAGCTTCC CTGTCTCCAT TGTGCAGGCG AACAGCGCCG GGCAGCGAGA AGAATACCTG 
CTGTGCTTCC ACGAATTTGG GGTGTTCGTG GATTCTTACG GAAGACGTAG CCGCACAGAT 
GATCTCAAGT GGAGTCGCTT ACCTCTGGCC TTCGCCTACA GAGAACCTTA TCTGTTTGTG 
ACTCACTTCA ACTCCCTGGA AGTCATTGAG ATCCAGGCCA GATCCTCACT GGGGAGCCCT 
GCCCGAGCAT ATCTGGAAAT TCCAAACCCT CGCTACCTGG GCCCCGCGAT TTCCTCCGGA 
GCGATTTACC TGGCCTCCTC ATACCAGGAC AAGTTAAGGG TCATATGCTG CAAAGGAAAC 
CTCGTGAAGG AGTCAGGCAC TGAGCAGCAC CGGGTGCCCT CCACCTCCCG CAGCAGCCCC 
AACAAGCGAG GCCCACCAAC ATACAACGAG CACATCACCA AACGCGTGGC CTCCAGCCCG 
GCGCCACCGG AAGGCCCCAG CCACCCCCGA GAGCCAAGCA CACCGCACCG CTACCGAGAC 
AGAGAGGGTC GGACAGAGCT GCGCAGGGAC AAGTCTCCAG GCCGCCCTCT GGAGCGGGAG 
AAGTCCCCAG GCCGAATGCT CAGCACTAGG AGAGAGCGGT CCCCAGGGAG ACTGTTTGAA 
GACAGCAGCA GGGGCCGGCT GCCTGCAGGA GCAGTGAGGA CCCCACTGTC CCAGGTTAAC 
AAGGTCTGGG ACCAGTCTTC AGTATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017176131.1
CDS	1475..6361
Translation	MEQEMTRLHRRVSEVEAVLSQKEVELKASETQRSLLEQDLATYITECSSLKRSLEQARMEVSQEDDKALQLLHDIREQSRKLQEIKEQEYQAQVEEMRLMMNQLEEDLVSARRRSDLYESELRESRLAAEEFKRKANECQHKLMKAKDQGKPEVGEYSKLEKINAEQQLKIQELQEKLEKAVKASTEATELLQNIRQAKERAERELEKLHNREDSSEGIKKKLVEAEERRHSLENKVKRLETMERRENRLKDDIQTKSEQIQQMADKILELEEKHREAQVSAQHLEVHLKQKEQHYEEKIKVLDNQIKKDLADKESLENMMQRHEEEAHEKGKILSEQKAMINAMDSKIRSLEQRIVELSEANKLAANSSLFTQRNMKAQEEMISELRQQKFYLETQAGKLEAQNRKLEEQLEKISHQDHSDKSRLLELETRLREVSLEHEEQKLELKRQLTELQLSLQERESQLTALQAARAALESQLRQAKTELEETTAEAEEEIQALTAHRDEIQRKFDALRNSCTVITDLEEQLNQLTEDNAELNNQNFYLSKQLDEASGANDEIVQLRSEVDHLRREITEREMQLTSQKQAQLSAPDLQTMEALKTTCTMLEEQVLDLEALNDELLEKERQWEAWRSVLGDEKSQFECRVRELQRMLDTEKQSRARADQRITESRQVVELAVKEHKAEILALQQALKEQKLKAESLSDKLNDLEKKHAMLEMNARSLQQKLETERELKQRLLEEQAKLQQQMDLQKNHIFRLTQGLQEALDRADLLKTERSDLEYQLENIQVLYSHEKVKMEGTISQQTKLIDFLQAKMDQPAKKKKGLFSRRKEDPALPTQVPLQYNELKLALEKEKARCAELEEALQKTRIELRSAREEAAHRKATDHPHPSTPATARQQIAMSAIVRSPEHQPSAMSLLAPPSSRRKESSTPEEFSRRLKERMHHNIPHRFNVGLNMRATKCAVCLDTVHFGRQASKCLECQVMCHPKCSTCLPATCGLPAEYATHFTEAFCRDKMNSPGLQSKEPGSSLHLEGWMKVPRNNKRGQQGWDRKYIVLEGSKVLIYDNEAREAGQRPVEEFELCLPDGDVSIHGAVGASELANTAKADVPYILKMESHPHTTCWPGRTLYLLAPSFPDKQRWVTALESVVAGGRVSREKAEADAARDHTSSEHQPVWVEKLLGNSLLKLEGDDRLDMNCTLPFSDQVVLVGTEEGLYALNVLKNSLTHIPGIGAVFQIYIIKDLEKLLMIAGEERALCLVDVKKVKQSLAQSHLPAQPDVSPNIFEAVKGCHLFAAGKIENSLCICAAMPSKVVILRYNDNLSKYCIRKEIETSEPCSCIHFTNYSILIGTNKFYEIDMKQYTLDEFLDKNDHSLAPAVFASSSNSFPVSIVQANSAGQREEYLLCFHEFGVFVDSYGRRSRTDDLKWSRLPLAFAYREPYLFVTHFNSLEVIEIQARSSLGSPARAYLEIPNPRYLGPAISSGAIYLASSYQDKLRVICCKGNLVKESGTEQHRVPSTSRSSPNKRGPPTYNEHITKRVASSPAPPEGPSHPREPSTPHRYRDREGRTELRRDKSPGRPLEREKSPGRMLSTRRERSPGRLFEDSSRGRLPAGAVRTPLSQVNKVWDQSSV

Target ORF information:

RefSeq Version	XM_017320642.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus citron (Cit), transcript variant X24, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017320642.1

ORF Insert Sequence:

ATGGAGCAGG AAATGACCCG GTTACATCGC AGAGTGTCAG AGGTGGAGGC TGTGCTTAGT 
CAGAAGGAGG TGGAGCTGAA GGCCTCTGAG ACTCAGAGAT CCCTCCTGGA GCAGGACCTT 
GCTACCTACA TCACAGAATG CAGTAGCTTA AAGCGAAGTT TGGAGCAAGC GCGGATGGAG 
GTGTCCCAGG AGGATGACAA AGCTCTGCAG CTTCTCCACG ACATCCGAGA GCAGAGCCGG 
AAGCTCCAGG AGATCAAGGA GCAGGAGTAC CAGGCTCAGG TGGAGGAGAT GAGGCTGATG 
ATGAATCAGC TGGAAGAAGA CCTGGTGTCA GCCCGCAGAC GCAGCGATCT CTACGAGTCT 
GAGCTGAGGG AGTCTCGGCT TGCCGCCGAG GAATTCAAGC GGAAGGCAAA CGAATGTCAG 
CACAAACTGA TGAAGGCTAA GGACCAAGGG AAGCCTGAAG TGGGAGAATA TTCCAAACTG 
GAGAAGATCA ATGCTGAGCA GCAGCTGAAG ATCCAGGAGC TCCAGGAGAA GCTGGAGAAG 
GCTGTAAAAG CCAGCACAGA GGCCACCGAG CTCCTGCAGA ATATCCGCCA GGCAAAGGAG 
CGAGCAGAGC GGGAGCTAGA GAAGCTACAC AACCGGGAAG ACTCCTCCGA GGGCATCAAA 
AAGAAGCTGG TGGAAGCCGA GGAACGCCGC CACTCCCTGG AGAACAAGGT AAAGAGACTA 
GAGACCATGG AGCGTAGAGA GAACAGACTG AAGGATGACA TCCAGACAAA GTCCGAACAG 
ATCCAGCAGA TGGCTGATAA AATTCTGGAG CTGGAGGAGA AACATCGGGA GGCTCAGGTC 
TCAGCTCAAC ATCTAGAAGT ACACTTGAAA CAGAAAGAAC AGCACTACGA GGAAAAGATC 
AAGGTATTGG ACAATCAGAT AAAGAAGGAC CTGGCCGACA AGGAGAGCCT GGAGAACATG 
ATGCAGAGAC ACGAGGAGGA GGCCCACGAG AAGGGCAAGA TCCTCAGCGA GCAGAAGGCG 
ATGATCAACG CGATGGATTC CAAGATCCGA TCCCTGGAGC AGAGGATCGT GGAGCTGTCG 
GAAGCCAACA AGCTTGCGGC AAACAGCAGT CTCTTCACCC AGAGGAACAT GAAGGCCCAG 
GAAGAGATGA TCTCAGAACT CAGGCAGCAG AAATTTTACC TGGAGACGCA GGCCGGAAAG 
CTGGAGGCCC AGAACCGAAA GCTGGAAGAG CAACTGGAGA AGATCAGCCA CCAAGATCAC 
AGTGACAAGA GTCGGCTGCT GGAGCTGGAA ACAAGGCTGA GGGAGGTCAG CCTGGAGCAC 
GAGGAGCAGA AGCTGGAGCT GAAGCGGCAG CTCACGGAGC TGCAGCTGTC CCTGCAGGAG 
CGCGAGTCCC AGCTGACGGC CCTGCAGGCC GCCCGGGCAG CGCTGGAGAG CCAGCTCCGC 
CAGGCGAAGA CAGAGCTGGA GGAGACAACC GCGGAAGCGG AGGAGGAGAT CCAGGCGCTC 
ACGGCACATC GAGATGAGAT CCAGCGCAAA TTCGATGCCC TTCGCAACAG CTGCACGGTC 
ATCACCGACC TGGAGGAGCA GCTGAACCAG CTCACCGAGG ACAACGCCGA GCTCAACAAC 
CAAAACTTCT ACCTGTCCAA ACAACTCGAT GAGGCTTCCG GGGCCAATGA CGAGATTGTG 
CAGCTGCGGA GTGAGGTGGA CCATCTCCGC CGTGAGATCA CGGAGCGGGA GATGCAGCTC 
ACCAGCCAGA AGCAAGCACA ACTCAGTGCT CCTGATTTGC AGACGATGGA GGCTCTGAAG 
ACGACATGCA CCATGCTGGA GGAGCAGGTC CTGGACCTGG AGGCCCTGAA CGACGAGCTG 
CTGGAGAAGG AGCGCCAGTG GGAGGCCTGG CGGAGCGTCC TCGGCGACGA GAAGTCCCAG 
TTCGAGTGTC GAGTTCGAGA GCTACAGAGG ATGCTGGACA CCGAGAAGCA GAGCAGGGCT 
AGGGCCGATC AGCGGATCAC CGAGTCGCGC CAGGTGGTGG AGTTGGCGGT GAAGGAACAC 
AAGGCCGAGA TTCTTGCTCT GCAGCAGGCT CTCAAGGAGC AGAAGCTCAA AGCCGAGAGC 
CTGTCGGACA AGCTCAACGA CCTGGAGAAG AAGCACGCCA TGCTGGAGAT GAACGCCCGG 
AGCTTACAGC AGAAACTAGA GACAGAGCGG GAGCTCAAAC AGAGGCTTCT GGAGGAGCAA 
GCCAAATTGC AGCAGCAGAT GGACCTGCAG AAGAACCACA TCTTCAGACT GACGCAAGGG 
CTGCAGGAGG CGCTGGACCG GGCGGATCTG CTGAAGACAG AAAGGAGCGA CCTGGAGTAC 
CAGCTGGAAA ACATTCAGGT TCTCTACTCT CACGAGAAAG TGAAAATGGA AGGCACAATC 
TCTCAGCAAA CCAAACTCAT TGATTTCCTG CAAGCCAAAA TGGACCAGCC TGCTAAAAAG 
AAAAAGGGTT TATTTAGTCG ACGGAAAGAG GACCCTGCTT TGCCCACACA GGTTCCTCTG 
CAGTACAATG AGCTGAAGCT AGCCCTGGAA AAGGAGAAAG CCCGATGCGC GGAGCTGGAG 
GAGGCCCTTC AGAAGACCCG CATCGAACTC CGCTCTGCGC GGGAGGAAGC TGCCCACCGC 
AAAGCCACGG ACCACCCGCA CCCGTCTACG CCAGCCACTG CGAGGCAGCA GATCGCCATG 
TCTGCCATTG TGCGGTCGCC CGAGCACCAG CCCAGTGCCA TGAGCCTGCT TGCCCCACCA 
TCCAGCCGCA GAAAGGAGTC GTCAACTCCA GAGGAATTCA GCCGGCGTCT GAAAGAGCGC 
ATGCACCACA ACATCCCTCA CCGGTTTAAT GTGGGCCTGA ACATGAGAGC CACCAAGTGC 
GCCGTGTGTC TGGATACTGT GCACTTTGGA CGCCAGGCAT CCAAATGCCT CGAATGTCAA 
GTGATGTGTC ACCCCAAATG CTCCACCTGC TTGCCCGCTA CCTGTGGCCT GCCAGCTGAA 
TATGCCACAC ACTTCACTGA GGCCTTCTGC CGGGACAAAA TGAACTCCCC GGGGCTCCAG 
AGCAAGGAGC CTGGCAGCAG CTTGCACCTG GAAGGGTGGA TGAAAGTGCC CAGAAATAAC 
AAACGGGGAC AGCAAGGCTG GGACAGGAAG TACATTGTTC TGGAGGGGTC AAAAGTCCTC 
ATCTATGACA ATGAAGCCAG AGAAGCTGGA CAGAGGCCGG TGGAAGAATT TGAGCTGTGC 
CTTCCTGACG GGGACGTATC TATTCATGGT GCCGTTGGTG CTTCAGAACT TGCAAATACC 
GCCAAAGCAG ATGTCCCATA CATCCTGAAG ATGGAGTCTC ACCCACACAC CACCTGCTGG 
CCTGGGAGAA CCCTCTACTT GCTAGCACCC AGCTTCCCCG ACAAGCAGCG CTGGGTCACC 
GCCTTAGAAT CTGTCGTCGC AGGTGGGAGA GTTTCTAGGG AAAAGGCCGA AGCCGATGCT 
GCACGTGACC ATACTTCGAG TGAGCATCAG CCAGTGTGGG TTGAGAAATT ACTTGGAAAC 
TCTCTGCTGA AACTGGAGGG CGATGACCGG CTTGACATGA ACTGCACCCT GCCCTTCAGT 
GACCAGGTAG TGCTGGTGGG CACGGAGGAA GGCCTCTACG CCCTGAATGT CTTGAAAAAC 
TCCTTAACCC ACATCCCAGG GATTGGCGCA GTCTTCCAAA TTTACATCAT CAAGGACCTG 
GAGAAGCTGC TCATGATAGC AGGGGAAGAG CGGGCTCTGT GCCTGGTGGA CGTGAAGAAG 
GTGAAGCAGT CCCTGGCGCA GTCACACCTG CCTGCCCAGC CCGACGTCTC CCCCAACATA 
TTCGAAGCCG TCAAAGGCTG CCACTTGTTC GCTGCTGGCA AGATCGAGAA CAGCCTGTGC 
ATCTGCGCCG CTATGCCAAG CAAAGTCGTC ATCCTCCGCT ACAATGACAA CCTCAGCAAG 
TACTGCATCC GCAAGGAGAT CGAGACCTCA GAGCCCTGCA GCTGTATCCA CTTCACCAAC 
TACAGCATCC TCATTGGGAC CAACAAATTC TATGAGATCG ACATGAAGCA GTACACGCTT 
GACGAGTTCC TGGACAAGAA CGACCATTCC TTGGCACCTG CTGTGTTCGC CTCCTCGTCC 
AACAGCTTCC CTGTCTCCAT TGTGCAGGCG AACAGCGCCG GGCAGCGAGA AGAATACCTG 
CTGTGCTTCC ACGAATTTGG GGTGTTCGTG GATTCTTACG GAAGACGTAG CCGCACAGAT 
GATCTCAAGT GGAGTCGCTT ACCTCTGGCC TTCGCCTACA GAGAACCTTA TCTGTTTGTG 
ACTCACTTCA ACTCCCTGGA AGTCATTGAG ATCCAGGCCA GATCCTCACT GGGGAGCCCT 
GCCCGAGCAT ATCTGGAAAT TCCAAACCCT CGCTACCTGG GCCCCGCGAT TTCCTCCGGA 
GCGATTTACC TGGCCTCCTC ATACCAGGAC AAGTTAAGGG TCATATGCTG CAAAGGAAAC 
CTCGTGAAGG AGTCAGGCAC TGAGCAGCAC CGGGTGCCCT CCACCTCCCG CAGCAGCCCC 
AACAAGCGAG GCCCACCAAC ATACAACGAG CACATCACCA AACGCGTGGC CTCCAGCCCG 
GCGCCACCGG AAGGCCCCAG CCACCCCCGA GAGCCAAGCA CACCGCACCG CTACCGAGAC 
AGAGAGGGTC GGACAGAGCT GCGCAGGGAC AAGTCTCCAG GCCGCCCTCT GGAGCGGGAG 
AAGTCCCCAG GCCGAATGCT CAGCACTAGG AGAGAGCGGT CCCCAGGGAG ACTGTTTGAA 
GACAGCAGCA GGGGCCGGCT GCCTGCAGGA GCAGTGAGGA CCCCACTGTC CCAGGTTAAC 
AAGGTCTGGG ACCAGTCTTC AGTATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cit ( XM_017320643.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017176132.1 Mus musculus citron (Cit), transcript variant X27, mRNA.

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CloneID	OMu81234
Clone ID Related Accession (Same CDS sequence)	XM_017320643.2 , XM_017320643.1
Accession Version	XM_017320643.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4884bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	citron Rho-interacting kinase isoform X22
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000071.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_017320643.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTCCTGG GCGAAGAAGC AATGATGGAG CAGGAAATGA CCCGGTTACA TCGCAGAGTG 
TCAGAGGTGG AGGCTGTGCT TAGTCAGAAG GAGGTGGAGC TGAAGGCCTC TGAGACTCAG 
AGATCCCTCC TGGAGCAGGA CCTTGCTACC TACATCACAG AATGCAGTAG CTTAAAGCGA 
AGTTTGGAGC AAGCGCGGAT GGAGGTGTCC CAGGAGGATG ACAAAGCTCT GCAGCTTCTC 
CACGACATCC GAGAGCAGAG CCGGAAGCTC CAGGAGATCA AGGAGCAGGA GTACCAGGCT 
CAGGTGGAGG AGATGAGGCT GATGATGAAT CAGCTGGAAG AAGACCTGGT GTCAGCCCGC 
AGACGCAGCG ATCTCTACGA GTCTGAGCTG AGGGAGTCTC GGCTTGCCGC CGAGGAATTC 
AAGCGGAAGG CAAACGAATG TCAGCACAAA CTGATGAAGG CTAAGGACCA AGGGAAGCCT 
GAAGTGGGAG AATATTCCAA ACTGGAGAAG ATCAATGCTG AGCAGCAGCT GAAGATCCAG 
GAGCTCCAGG AGAAGCTGGA GAAGGCTGTA AAAGCCAGCA CAGAGGCCAC CGAGCTCCTG 
CAGAATATCC GCCAGGCAAA GGAGCGAGCA GAGCGGGAGC TAGAGAAGCT ACACAACCGG 
GAAGACTCCT CCGAGGGCAT CAAAAAGAAG CTGGTGGAAG CCGAGGAACG CCGCCACTCC 
CTGGAGAACA AGGTAAAGAG ACTAGAGACC ATGGAGCGTA GAGAGAACAG ACTGAAGGAT 
GACATCCAGA CAAAGTCCGA ACAGATCCAG CAGATGGCTG ATAAAATTCT GGAGCTGGAG 
GAGAAACATC GGGAGGCTCA GGTCTCAGCT CAACATCTAG AAGTACACTT GAAACAGAAA 
GAACAGCACT ACGAGGAAAA GATCAAGGTA TTGGACAATC AGATAAAGAA GGACCTGGCC 
GACAAGGAGA GCCTGGAGAA CATGATGCAG AGACACGAGG AGGAGGCCCA CGAGAAGGGC 
AAGATCCTCA GCGAGCAGAA GGCGATGATC AACGCGATGG ATTCCAAGAT CCGATCCCTG 
GAGCAGAGGA TCGTGGAGCT GTCGGAAGCC AACAAGCTTG CGGCAAACAG CAGTCTCTTC 
ACCCAGAGGA ACATGAAGGC CCAGGAAGAG ATGATCTCAG AACTCAGGCA GCAGAAATTT 
TACCTGGAGA CGCAGGCCGG AAAGCTGGAG GCCCAGAACC GAAAGCTGGA AGAGCAACTG 
GAGAAGATCA GCCACCAAGA TCACAGTGAC AAGAGTCGGC TGCTGGAGCT GGAAACAAGG 
CTGAGGGAGG TCAGCCTGGA GCACGAGGAG CAGAAGCTGG AGCTGAAGCG GCAGCTCACG 
GAGCTGCAGC TGTCCCTGCA GGAGCGCGAG TCCCAGCTGA CGGCCCTGCA GGCCGCCCGG 
GCAGCGCTGG AGAGCCAGCT CCGCCAGGCG AAGACAGAGC TGGAGGAGAC AACCGCGGAA 
GCGGAGGAGG AGATCCAGGC GCTCACGGCA CATCGAGATG AGATCCAGCG CAAATTCGAT 
GCCCTTCGCA ACAGCTGCAC GGTCATCACC GACCTGGAGG AGCAGCTGAA CCAGCTCACC 
GAGGACAACG CCGAGCTCAA CAACCAAAAC TTCTACCTGT CCAAACAACT CGATGAGGCT 
TCCGGGGCCA ATGACGAGAT TGTGCAGCTG CGGAGTGAGG TGGACCATCT CCGCCGTGAG 
ATCACGGAGC GGGAGATGCA GCTCACCAGC CAGAAGCAAA CGATGGAGGC TCTGAAGACG 
ACATGCACCA TGCTGGAGGA GCAGGTCCTG GACCTGGAGG CCCTGAACGA CGAGCTGCTG 
GAGAAGGAGC GCCAGTGGGA GGCCTGGCGG AGCGTCCTCG GCGACGAGAA GTCCCAGTTC 
GAGTGTCGAG TTCGAGAGCT ACAGAGGATG CTGGACACCG AGAAGCAGAG CAGGGCTAGG 
GCCGATCAGC GGATCACCGA GTCGCGCCAG GTGGTGGAGT TGGCGGTGAA GGAACACAAG 
GCCGAGATTC TTGCTCTGCA GCAGGCTCTC AAGGAGCAGA AGCTCAAAGC CGAGAGCCTG 
TCGGACAAGC TCAACGACCT GGAGAAGAAG CACGCCATGC TGGAGATGAA CGCCCGGAGC 
TTACAGCAGA AACTAGAGAC AGAGCGGGAG CTCAAACAGA GGCTTCTGGA GGAGCAAGCC 
AAATTGCAGC AGCAGATGGA CCTGCAGAAG AACCACATCT TCAGACTGAC GCAAGGGCTG 
CAGGAGGCGC TGGACCGGGC GGATCTGCTG AAGACAGAAA GGAGCGACCT GGAGTACCAG 
CTGGAAAACA TTCAGGTTCT CTACTCTCAC GAGAAAGTGA AAATGGAAGG CACAATCTCT 
CAGCAAACCA AACTCATTGA TTTCCTGCAA GCCAAAATGG ACCAGCCTGC TAAAAAGAAA 
AAGGGTTTAT TTAGTCGACG GAAAGAGGAC CCTGCTTTGC CCACACAGGT TCCTCTGCAG 
TACAATGAGC TGAAGCTAGC CCTGGAAAAG GAGAAAGCCC GATGCGCGGA GCTGGAGGAG 
GCCCTTCAGA AGACCCGCAT CGAACTCCGC TCTGCGCGGG AGGAAGCTGC CCACCGCAAA 
GCCACGGACC ACCCGCACCC GTCTACGCCA GCCACTGCGA GGCAGCAGAT CGCCATGTCT 
GCCATTGTGC GGTCGCCCGA GCACCAGCCC AGTGCCATGA GCCTGCTTGC CCCACCATCC 
AGCCGCAGAA AGGAGTCGTC AACTCCAGAG GAATTCAGCC GGCGTCTGAA AGAGCGCATG 
CACCACAACA TCCCTCACCG GTTTAATGTG GGCCTGAACA TGAGAGCCAC CAAGTGCGCC 
GTGTGTCTGG ATACTGTGCA CTTTGGACGC CAGGCATCCA AATGCCTCGA ATGTCAAGTG 
ATGTGTCACC CCAAATGCTC CACCTGCTTG CCCGCTACCT GTGGCCTGCC AGCTGAATAT 
GCCACACACT TCACTGAGGC CTTCTGCCGG GACAAAATGA ACTCCCCGGG GCTCCAGAGC 
AAGGAGCCTG GCAGCAGCTT GCACCTGGAA GGGTGGATGA AAGTGCCCAG AAATAACAAA 
CGGGGACAGC AAGGCTGGGA CAGGAAGTAC ATTGTTCTGG AGGGGTCAAA AGTCCTCATC 
TATGACAATG AAGCCAGAGA AGCTGGACAG AGGCCGGTGG AAGAATTTGA GCTGTGCCTT 
CCTGACGGGG ACGTATCTAT TCATGGTGCC GTTGGTGCTT CAGAACTTGC AAATACCGCC 
AAAGCAGATG TCCCATACAT CCTGAAGATG GAGTCTCACC CACACACCAC CTGCTGGCCT 
GGGAGAACCC TCTACTTGCT AGCACCCAGC TTCCCCGACA AGCAGCGCTG GGTCACCGCC 
TTAGAATCTG TCGTCGCAGG TGGGAGAGTT TCTAGGGAAA AGGCCGAAGC CGATGCTGCA 
CGTGACCATA CTTCGAGTGA GCATCAGCCA GTGTGGGTTG AGAAATTACT TGGAAACTCT 
CTGCTGAAAC TGGAGGGCGA TGACCGGCTT GACATGAACT GCACCCTGCC CTTCAGTGAC 
CAGGTAGTGC TGGTGGGCAC GGAGGAAGGC CTCTACGCCC TGAATGTCTT GAAAAACTCC 
TTAACCCACA TCCCAGGGAT TGGCGCAGTC TTCCAAATTT ACATCATCAA GGACCTGGAG 
AAGCTGCTCA TGATAGCAGG GGAAGAGCGG GCTCTGTGCC TGGTGGACGT GAAGAAGGTG 
AAGCAGTCCC TGGCGCAGTC ACACCTGCCT GCCCAGCCCG ACGTCTCCCC CAACATATTC 
GAAGCCGTCA AAGGCTGCCA CTTGTTCGCT GCTGGCAAGA TCGAGAACAG CCTGTGCATC 
TGCGCCGCTA TGCCAAGCAA AGTCGTCATC CTCCGCTACA ATGACAACCT CAGCAAGTAC 
TGCATCCGCA AGGAGATCGA GACCTCAGAG CCCTGCAGCT GTATCCACTT CACCAACTAC 
AGCATCCTCA TTGGGACCAA CAAATTCTAT GAGATCGACA TGAAGCAGTA CACGCTTGAC 
GAGTTCCTGG ACAAGAACGA CCATTCCTTG GCACCTGCTG TGTTCGCCTC CTCGTCCAAC 
AGCTTCCCTG TCTCCATTGT GCAGGCGAAC AGCGCCGGGC AGCGAGAAGA ATACCTGCTG 
TGCTTCCACG AATTTGGGGT GTTCGTGGAT TCTTACGGAA GACGTAGCCG CACAGATGAT 
CTCAAGTGGA GTCGCTTACC TCTGGCCTTC GCCTACAGAG AACCTTATCT GTTTGTGACT 
CACTTCAACT CCCTGGAAGT CATTGAGATC CAGGCCAGAT CCTCACTGGG GAGCCCTGCC 
CGAGCATATC TGGAAATTCC AAACCCTCGC TACCTGGGCC CCGCGATTTC CTCCGGAGCG 
ATTTACCTGG CCTCCTCATA CCAGGACAAG TTAAGGGTCA TATGCTGCAA AGGAAACCTC 
GTGAAGGAGT CAGGCACTGA GCAGCACCGG GTGCCCTCCA CCTCCCGCAG CAGCCCCAAC 
AAGCGAGGCC CACCAACATA CAACGAGCAC ATCACCAAAC GCGTGGCCTC CAGCCCGGCG 
CCACCGGAAG GCCCCAGCCA CCCCCGAGAG CCAAGCACAC CGCACCGCTA CCGAGACAGA 
GAGGGTCGGA CAGAGCTGCG CAGGGACAAG TCTCCAGGCC GCCCTCTGGA GCGGGAGAAG 
TCCCCAGGCC GAATGCTCAG CACTAGGAGA GAGCGGTCCC CAGGGAGACT GTTTGAAGAC 
AGCAGCAGGG GCCGGCTGCC TGCAGGAGCA GTGAGGACCC CACTGTCCCA GGTTAACAAG 
GTCTGGGACC AGTCTTCAGT ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017176132.1
CDS	1147..6030
Translation	MLLGEEAMMEQEMTRLHRRVSEVEAVLSQKEVELKASETQRSLLEQDLATYITECSSLKRSLEQARMEVSQEDDKALQLLHDIREQSRKLQEIKEQEYQAQVEEMRLMMNQLEEDLVSARRRSDLYESELRESRLAAEEFKRKANECQHKLMKAKDQGKPEVGEYSKLEKINAEQQLKIQELQEKLEKAVKASTEATELLQNIRQAKERAERELEKLHNREDSSEGIKKKLVEAEERRHSLENKVKRLETMERRENRLKDDIQTKSEQIQQMADKILELEEKHREAQVSAQHLEVHLKQKEQHYEEKIKVLDNQIKKDLADKESLENMMQRHEEEAHEKGKILSEQKAMINAMDSKIRSLEQRIVELSEANKLAANSSLFTQRNMKAQEEMISELRQQKFYLETQAGKLEAQNRKLEEQLEKISHQDHSDKSRLLELETRLREVSLEHEEQKLELKRQLTELQLSLQERESQLTALQAARAALESQLRQAKTELEETTAEAEEEIQALTAHRDEIQRKFDALRNSCTVITDLEEQLNQLTEDNAELNNQNFYLSKQLDEASGANDEIVQLRSEVDHLRREITEREMQLTSQKQTMEALKTTCTMLEEQVLDLEALNDELLEKERQWEAWRSVLGDEKSQFECRVRELQRMLDTEKQSRARADQRITESRQVVELAVKEHKAEILALQQALKEQKLKAESLSDKLNDLEKKHAMLEMNARSLQQKLETERELKQRLLEEQAKLQQQMDLQKNHIFRLTQGLQEALDRADLLKTERSDLEYQLENIQVLYSHEKVKMEGTISQQTKLIDFLQAKMDQPAKKKKGLFSRRKEDPALPTQVPLQYNELKLALEKEKARCAELEEALQKTRIELRSAREEAAHRKATDHPHPSTPATARQQIAMSAIVRSPEHQPSAMSLLAPPSSRRKESSTPEEFSRRLKERMHHNIPHRFNVGLNMRATKCAVCLDTVHFGRQASKCLECQVMCHPKCSTCLPATCGLPAEYATHFTEAFCRDKMNSPGLQSKEPGSSLHLEGWMKVPRNNKRGQQGWDRKYIVLEGSKVLIYDNEAREAGQRPVEEFELCLPDGDVSIHGAVGASELANTAKADVPYILKMESHPHTTCWPGRTLYLLAPSFPDKQRWVTALESVVAGGRVSREKAEADAARDHTSSEHQPVWVEKLLGNSLLKLEGDDRLDMNCTLPFSDQVVLVGTEEGLYALNVLKNSLTHIPGIGAVFQIYIIKDLEKLLMIAGEERALCLVDVKKVKQSLAQSHLPAQPDVSPNIFEAVKGCHLFAAGKIENSLCICAAMPSKVVILRYNDNLSKYCIRKEIETSEPCSCIHFTNYSILIGTNKFYEIDMKQYTLDEFLDKNDHSLAPAVFASSSNSFPVSIVQANSAGQREEYLLCFHEFGVFVDSYGRRSRTDDLKWSRLPLAFAYREPYLFVTHFNSLEVIEIQARSSLGSPARAYLEIPNPRYLGPAISSGAIYLASSYQDKLRVICCKGNLVKESGTEQHRVPSTSRSSPNKRGPPTYNEHITKRVASSPAPPEGPSHPREPSTPHRYRDREGRTELRRDKSPGRPLEREKSPGRMLSTRRERSPGRLFEDSSRGRLPAGAVRTPLSQVNKVWDQSSV

Target ORF information:

RefSeq Version	XM_017320643.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus citron (Cit), transcript variant X27, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017320643.2

ORF Insert Sequence:

ATGCTCCTGG GCGAAGAAGC AATGATGGAG CAGGAAATGA CCCGGTTACA TCGCAGAGTG 
TCAGAGGTGG AGGCTGTGCT TAGTCAGAAG GAGGTGGAGC TGAAGGCCTC TGAGACTCAG 
AGATCCCTCC TGGAGCAGGA CCTTGCTACC TACATCACAG AATGCAGTAG CTTAAAGCGA 
AGTTTGGAGC AAGCGCGGAT GGAGGTGTCC CAGGAGGATG ACAAAGCTCT GCAGCTTCTC 
CACGACATCC GAGAGCAGAG CCGGAAGCTC CAGGAGATCA AGGAGCAGGA GTACCAGGCT 
CAGGTGGAGG AGATGAGGCT GATGATGAAT CAGCTGGAAG AAGACCTGGT GTCAGCCCGC 
AGACGCAGCG ATCTCTACGA GTCTGAGCTG AGGGAGTCTC GGCTTGCCGC CGAGGAATTC 
AAGCGGAAGG CAAACGAATG TCAGCACAAA CTGATGAAGG CTAAGGACCA AGGGAAGCCT 
GAAGTGGGAG AATATTCCAA ACTGGAGAAG ATCAATGCTG AGCAGCAGCT GAAGATCCAG 
GAGCTCCAGG AGAAGCTGGA GAAGGCTGTA AAAGCCAGCA CAGAGGCCAC CGAGCTCCTG 
CAGAATATCC GCCAGGCAAA GGAGCGAGCA GAGCGGGAGC TAGAGAAGCT ACACAACCGG 
GAAGACTCCT CCGAGGGCAT CAAAAAGAAG CTGGTGGAAG CCGAGGAACG CCGCCACTCC 
CTGGAGAACA AGGTAAAGAG ACTAGAGACC ATGGAGCGTA GAGAGAACAG ACTGAAGGAT 
GACATCCAGA CAAAGTCCGA ACAGATCCAG CAGATGGCTG ATAAAATTCT GGAGCTGGAG 
GAGAAACATC GGGAGGCTCA GGTCTCAGCT CAACATCTAG AAGTACACTT GAAACAGAAA 
GAACAGCACT ACGAGGAAAA GATCAAGGTA TTGGACAATC AGATAAAGAA GGACCTGGCC 
GACAAGGAGA GCCTGGAGAA CATGATGCAG AGACACGAGG AGGAGGCCCA CGAGAAGGGC 
AAGATCCTCA GCGAGCAGAA GGCGATGATC AACGCGATGG ATTCCAAGAT CCGATCCCTG 
GAGCAGAGGA TCGTGGAGCT GTCGGAAGCC AACAAGCTTG CGGCAAACAG CAGTCTCTTC 
ACCCAGAGGA ACATGAAGGC CCAGGAAGAG ATGATCTCAG AACTCAGGCA GCAGAAATTT 
TACCTGGAGA CGCAGGCCGG AAAGCTGGAG GCCCAGAACC GAAAGCTGGA AGAGCAACTG 
GAGAAGATCA GCCACCAAGA TCACAGTGAC AAGAGTCGGC TGCTGGAGCT GGAAACAAGG 
CTGAGGGAGG TCAGCCTGGA GCACGAGGAG CAGAAGCTGG AGCTGAAGCG GCAGCTCACG 
GAGCTGCAGC TGTCCCTGCA GGAGCGCGAG TCCCAGCTGA CGGCCCTGCA GGCCGCCCGG 
GCAGCGCTGG AGAGCCAGCT CCGCCAGGCG AAGACAGAGC TGGAGGAGAC AACCGCGGAA 
GCGGAGGAGG AGATCCAGGC GCTCACGGCA CATCGAGATG AGATCCAGCG CAAATTCGAT 
GCCCTTCGCA ACAGCTGCAC GGTCATCACC GACCTGGAGG AGCAGCTGAA CCAGCTCACC 
GAGGACAACG CCGAGCTCAA CAACCAAAAC TTCTACCTGT CCAAACAACT CGATGAGGCT 
TCCGGGGCCA ATGACGAGAT TGTGCAGCTG CGGAGTGAGG TGGACCATCT CCGCCGTGAG 
ATCACGGAGC GGGAGATGCA GCTCACCAGC CAGAAGCAAA CGATGGAGGC TCTGAAGACG 
ACATGCACCA TGCTGGAGGA GCAGGTCCTG GACCTGGAGG CCCTGAACGA CGAGCTGCTG 
GAGAAGGAGC GCCAGTGGGA GGCCTGGCGG AGCGTCCTCG GCGACGAGAA GTCCCAGTTC 
GAGTGTCGAG TTCGAGAGCT ACAGAGGATG CTGGACACCG AGAAGCAGAG CAGGGCTAGG 
GCCGATCAGC GGATCACCGA GTCGCGCCAG GTGGTGGAGT TGGCGGTGAA GGAACACAAG 
GCCGAGATTC TTGCTCTGCA GCAGGCTCTC AAGGAGCAGA AGCTCAAAGC CGAGAGCCTG 
TCGGACAAGC TCAACGACCT GGAGAAGAAG CACGCCATGC TGGAGATGAA CGCCCGGAGC 
TTACAGCAGA AACTAGAGAC AGAGCGGGAG CTCAAACAGA GGCTTCTGGA GGAGCAAGCC 
AAATTGCAGC AGCAGATGGA CCTGCAGAAG AACCACATCT TCAGACTGAC GCAAGGGCTG 
CAGGAGGCGC TGGACCGGGC GGATCTGCTG AAGACAGAAA GGAGCGACCT GGAGTACCAG 
CTGGAAAACA TTCAGGTTCT CTACTCTCAC GAGAAAGTGA AAATGGAAGG CACAATCTCT 
CAGCAAACCA AACTCATTGA TTTCCTGCAA GCCAAAATGG ACCAGCCTGC TAAAAAGAAA 
AAGGGTTTAT TTAGTCGACG GAAAGAGGAC CCTGCTTTGC CCACACAGGT TCCTCTGCAG 
TACAATGAGC TGAAGCTAGC CCTGGAAAAG GAGAAAGCCC GATGCGCGGA GCTGGAGGAG 
GCCCTTCAGA AGACCCGCAT CGAACTCCGC TCTGCGCGGG AGGAAGCTGC CCACCGCAAA 
GCCACGGACC ACCCGCACCC GTCTACGCCA GCCACTGCGA GGCAGCAGAT CGCCATGTCT 
GCCATTGTGC GGTCGCCCGA GCACCAGCCC AGTGCCATGA GCCTGCTTGC CCCACCATCC 
AGCCGCAGAA AGGAGTCGTC AACTCCAGAG GAATTCAGCC GGCGTCTGAA AGAGCGCATG 
CACCACAACA TCCCTCACCG GTTTAATGTG GGCCTGAACA TGAGAGCCAC CAAGTGCGCC 
GTGTGTCTGG ATACTGTGCA CTTTGGACGC CAGGCATCCA AATGCCTCGA ATGTCAAGTG 
ATGTGTCACC CCAAATGCTC CACCTGCTTG CCCGCTACCT GTGGCCTGCC AGCTGAATAT 
GCCACACACT TCACTGAGGC CTTCTGCCGG GACAAAATGA ACTCCCCGGG GCTCCAGAGC 
AAGGAGCCTG GCAGCAGCTT GCACCTGGAA GGGTGGATGA AAGTGCCCAG AAATAACAAA 
CGGGGACAGC AAGGCTGGGA CAGGAAGTAC ATTGTTCTGG AGGGGTCAAA AGTCCTCATC 
TATGACAATG AAGCCAGAGA AGCTGGACAG AGGCCGGTGG AAGAATTTGA GCTGTGCCTT 
CCTGACGGGG ACGTATCTAT TCATGGTGCC GTTGGTGCTT CAGAACTTGC AAATACCGCC 
AAAGCAGATG TCCCATACAT CCTGAAGATG GAGTCTCACC CACACACCAC CTGCTGGCCT 
GGGAGAACCC TCTACTTGCT AGCACCCAGC TTCCCCGACA AGCAGCGCTG GGTCACCGCC 
TTAGAATCTG TCGTCGCAGG TGGGAGAGTT TCTAGGGAAA AGGCCGAAGC CGATGCTGCA 
CGTGACCATA CTTCGAGTGA GCATCAGCCA GTGTGGGTTG AGAAATTACT TGGAAACTCT 
CTGCTGAAAC TGGAGGGCGA TGACCGGCTT GACATGAACT GCACCCTGCC CTTCAGTGAC 
CAGGTAGTGC TGGTGGGCAC GGAGGAAGGC CTCTACGCCC TGAATGTCTT GAAAAACTCC 
TTAACCCACA TCCCAGGGAT TGGCGCAGTC TTCCAAATTT ACATCATCAA GGACCTGGAG 
AAGCTGCTCA TGATAGCAGG GGAAGAGCGG GCTCTGTGCC TGGTGGACGT GAAGAAGGTG 
AAGCAGTCCC TGGCGCAGTC ACACCTGCCT GCCCAGCCCG ACGTCTCCCC CAACATATTC 
GAAGCCGTCA AAGGCTGCCA CTTGTTCGCT GCTGGCAAGA TCGAGAACAG CCTGTGCATC 
TGCGCCGCTA TGCCAAGCAA AGTCGTCATC CTCCGCTACA ATGACAACCT CAGCAAGTAC 
TGCATCCGCA AGGAGATCGA GACCTCAGAG CCCTGCAGCT GTATCCACTT CACCAACTAC 
AGCATCCTCA TTGGGACCAA CAAATTCTAT GAGATCGACA TGAAGCAGTA CACGCTTGAC 
GAGTTCCTGG ACAAGAACGA CCATTCCTTG GCACCTGCTG TGTTCGCCTC CTCGTCCAAC 
AGCTTCCCTG TCTCCATTGT GCAGGCGAAC AGCGCCGGGC AGCGAGAAGA ATACCTGCTG 
TGCTTCCACG AATTTGGGGT GTTCGTGGAT TCTTACGGAA GACGTAGCCG CACAGATGAT 
CTCAAGTGGA GTCGCTTACC TCTGGCCTTC GCCTACAGAG AACCTTATCT GTTTGTGACT 
CACTTCAACT CCCTGGAAGT CATTGAGATC CAGGCCAGAT CCTCACTGGG GAGCCCTGCC 
CGAGCATATC TGGAAATTCC AAACCCTCGC TACCTGGGCC CCGCGATTTC CTCCGGAGCG 
ATTTACCTGG CCTCCTCATA CCAGGACAAG TTAAGGGTCA TATGCTGCAA AGGAAACCTC 
GTGAAGGAGT CAGGCACTGA GCAGCACCGG GTGCCCTCCA CCTCCCGCAG CAGCCCCAAC 
AAGCGAGGCC CACCAACATA CAACGAGCAC ATCACCAAAC GCGTGGCCTC CAGCCCGGCG 
CCACCGGAAG GCCCCAGCCA CCCCCGAGAG CCAAGCACAC CGCACCGCTA CCGAGACAGA 
GAGGGTCGGA CAGAGCTGCG CAGGGACAAG TCTCCAGGCC GCCCTCTGGA GCGGGAGAAG 
TCCCCAGGCC GAATGCTCAG CACTAGGAGA GAGCGGTCCC CAGGGAGACT GTTTGAAGAC 
AGCAGCAGGG GCCGGCTGCC TGCAGGAGCA GTGAGGACCC CACTGTCCCA GGTTAACAAG 
GTCTGGGACC AGTCTTCAGT ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cit ( XM_017320643.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017176132.1 Mus musculus citron (Cit), transcript variant X25, mRNA.

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CloneID	OMu81234
Clone ID Related Accession (Same CDS sequence)	XM_017320643.2 , XM_017320643.1
Accession Version	XM_017320643.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4884bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	citron Rho-interacting kinase isoform X21
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000071.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTCCTGG GCGAAGAAGC AATGATGGAG CAGGAAATGA CCCGGTTACA TCGCAGAGTG 
TCAGAGGTGG AGGCTGTGCT TAGTCAGAAG GAGGTGGAGC TGAAGGCCTC TGAGACTCAG 
AGATCCCTCC TGGAGCAGGA CCTTGCTACC TACATCACAG AATGCAGTAG CTTAAAGCGA 
AGTTTGGAGC AAGCGCGGAT GGAGGTGTCC CAGGAGGATG ACAAAGCTCT GCAGCTTCTC 
CACGACATCC GAGAGCAGAG CCGGAAGCTC CAGGAGATCA AGGAGCAGGA GTACCAGGCT 
CAGGTGGAGG AGATGAGGCT GATGATGAAT CAGCTGGAAG AAGACCTGGT GTCAGCCCGC 
AGACGCAGCG ATCTCTACGA GTCTGAGCTG AGGGAGTCTC GGCTTGCCGC CGAGGAATTC 
AAGCGGAAGG CAAACGAATG TCAGCACAAA CTGATGAAGG CTAAGGACCA AGGGAAGCCT 
GAAGTGGGAG AATATTCCAA ACTGGAGAAG ATCAATGCTG AGCAGCAGCT GAAGATCCAG 
GAGCTCCAGG AGAAGCTGGA GAAGGCTGTA AAAGCCAGCA CAGAGGCCAC CGAGCTCCTG 
CAGAATATCC GCCAGGCAAA GGAGCGAGCA GAGCGGGAGC TAGAGAAGCT ACACAACCGG 
GAAGACTCCT CCGAGGGCAT CAAAAAGAAG CTGGTGGAAG CCGAGGAACG CCGCCACTCC 
CTGGAGAACA AGGTAAAGAG ACTAGAGACC ATGGAGCGTA GAGAGAACAG ACTGAAGGAT 
GACATCCAGA CAAAGTCCGA ACAGATCCAG CAGATGGCTG ATAAAATTCT GGAGCTGGAG 
GAGAAACATC GGGAGGCTCA GGTCTCAGCT CAACATCTAG AAGTACACTT GAAACAGAAA 
GAACAGCACT ACGAGGAAAA GATCAAGGTA TTGGACAATC AGATAAAGAA GGACCTGGCC 
GACAAGGAGA GCCTGGAGAA CATGATGCAG AGACACGAGG AGGAGGCCCA CGAGAAGGGC 
AAGATCCTCA GCGAGCAGAA GGCGATGATC AACGCGATGG ATTCCAAGAT CCGATCCCTG 
GAGCAGAGGA TCGTGGAGCT GTCGGAAGCC AACAAGCTTG CGGCAAACAG CAGTCTCTTC 
ACCCAGAGGA ACATGAAGGC CCAGGAAGAG ATGATCTCAG AACTCAGGCA GCAGAAATTT 
TACCTGGAGA CGCAGGCCGG AAAGCTGGAG GCCCAGAACC GAAAGCTGGA AGAGCAACTG 
GAGAAGATCA GCCACCAAGA TCACAGTGAC AAGAGTCGGC TGCTGGAGCT GGAAACAAGG 
CTGAGGGAGG TCAGCCTGGA GCACGAGGAG CAGAAGCTGG AGCTGAAGCG GCAGCTCACG 
GAGCTGCAGC TGTCCCTGCA GGAGCGCGAG TCCCAGCTGA CGGCCCTGCA GGCCGCCCGG 
GCAGCGCTGG AGAGCCAGCT CCGCCAGGCG AAGACAGAGC TGGAGGAGAC AACCGCGGAA 
GCGGAGGAGG AGATCCAGGC GCTCACGGCA CATCGAGATG AGATCCAGCG CAAATTCGAT 
GCCCTTCGCA ACAGCTGCAC GGTCATCACC GACCTGGAGG AGCAGCTGAA CCAGCTCACC 
GAGGACAACG CCGAGCTCAA CAACCAAAAC TTCTACCTGT CCAAACAACT CGATGAGGCT 
TCCGGGGCCA ATGACGAGAT TGTGCAGCTG CGGAGTGAGG TGGACCATCT CCGCCGTGAG 
ATCACGGAGC GGGAGATGCA GCTCACCAGC CAGAAGCAAA CGATGGAGGC TCTGAAGACG 
ACATGCACCA TGCTGGAGGA GCAGGTCCTG GACCTGGAGG CCCTGAACGA CGAGCTGCTG 
GAGAAGGAGC GCCAGTGGGA GGCCTGGCGG AGCGTCCTCG GCGACGAGAA GTCCCAGTTC 
GAGTGTCGAG TTCGAGAGCT ACAGAGGATG CTGGACACCG AGAAGCAGAG CAGGGCTAGG 
GCCGATCAGC GGATCACCGA GTCGCGCCAG GTGGTGGAGT TGGCGGTGAA GGAACACAAG 
GCCGAGATTC TTGCTCTGCA GCAGGCTCTC AAGGAGCAGA AGCTCAAAGC CGAGAGCCTG 
TCGGACAAGC TCAACGACCT GGAGAAGAAG CACGCCATGC TGGAGATGAA CGCCCGGAGC 
TTACAGCAGA AACTAGAGAC AGAGCGGGAG CTCAAACAGA GGCTTCTGGA GGAGCAAGCC 
AAATTGCAGC AGCAGATGGA CCTGCAGAAG AACCACATCT TCAGACTGAC GCAAGGGCTG 
CAGGAGGCGC TGGACCGGGC GGATCTGCTG AAGACAGAAA GGAGCGACCT GGAGTACCAG 
CTGGAAAACA TTCAGGTTCT CTACTCTCAC GAGAAAGTGA AAATGGAAGG CACAATCTCT 
CAGCAAACCA AACTCATTGA TTTCCTGCAA GCCAAAATGG ACCAGCCTGC TAAAAAGAAA 
AAGGGTTTAT TTAGTCGACG GAAAGAGGAC CCTGCTTTGC CCACACAGGT TCCTCTGCAG 
TACAATGAGC TGAAGCTAGC CCTGGAAAAG GAGAAAGCCC GATGCGCGGA GCTGGAGGAG 
GCCCTTCAGA AGACCCGCAT CGAACTCCGC TCTGCGCGGG AGGAAGCTGC CCACCGCAAA 
GCCACGGACC ACCCGCACCC GTCTACGCCA GCCACTGCGA GGCAGCAGAT CGCCATGTCT 
GCCATTGTGC GGTCGCCCGA GCACCAGCCC AGTGCCATGA GCCTGCTTGC CCCACCATCC 
AGCCGCAGAA AGGAGTCGTC AACTCCAGAG GAATTCAGCC GGCGTCTGAA AGAGCGCATG 
CACCACAACA TCCCTCACCG GTTTAATGTG GGCCTGAACA TGAGAGCCAC CAAGTGCGCC 
GTGTGTCTGG ATACTGTGCA CTTTGGACGC CAGGCATCCA AATGCCTCGA ATGTCAAGTG 
ATGTGTCACC CCAAATGCTC CACCTGCTTG CCCGCTACCT GTGGCCTGCC AGCTGAATAT 
GCCACACACT TCACTGAGGC CTTCTGCCGG GACAAAATGA ACTCCCCGGG GCTCCAGAGC 
AAGGAGCCTG GCAGCAGCTT GCACCTGGAA GGGTGGATGA AAGTGCCCAG AAATAACAAA 
CGGGGACAGC AAGGCTGGGA CAGGAAGTAC ATTGTTCTGG AGGGGTCAAA AGTCCTCATC 
TATGACAATG AAGCCAGAGA AGCTGGACAG AGGCCGGTGG AAGAATTTGA GCTGTGCCTT 
CCTGACGGGG ACGTATCTAT TCATGGTGCC GTTGGTGCTT CAGAACTTGC AAATACCGCC 
AAAGCAGATG TCCCATACAT CCTGAAGATG GAGTCTCACC CACACACCAC CTGCTGGCCT 
GGGAGAACCC TCTACTTGCT AGCACCCAGC TTCCCCGACA AGCAGCGCTG GGTCACCGCC 
TTAGAATCTG TCGTCGCAGG TGGGAGAGTT TCTAGGGAAA AGGCCGAAGC CGATGCTGCA 
CGTGACCATA CTTCGAGTGA GCATCAGCCA GTGTGGGTTG AGAAATTACT TGGAAACTCT 
CTGCTGAAAC TGGAGGGCGA TGACCGGCTT GACATGAACT GCACCCTGCC CTTCAGTGAC 
CAGGTAGTGC TGGTGGGCAC GGAGGAAGGC CTCTACGCCC TGAATGTCTT GAAAAACTCC 
TTAACCCACA TCCCAGGGAT TGGCGCAGTC TTCCAAATTT ACATCATCAA GGACCTGGAG 
AAGCTGCTCA TGATAGCAGG GGAAGAGCGG GCTCTGTGCC TGGTGGACGT GAAGAAGGTG 
AAGCAGTCCC TGGCGCAGTC ACACCTGCCT GCCCAGCCCG ACGTCTCCCC CAACATATTC 
GAAGCCGTCA AAGGCTGCCA CTTGTTCGCT GCTGGCAAGA TCGAGAACAG CCTGTGCATC 
TGCGCCGCTA TGCCAAGCAA AGTCGTCATC CTCCGCTACA ATGACAACCT CAGCAAGTAC 
TGCATCCGCA AGGAGATCGA GACCTCAGAG CCCTGCAGCT GTATCCACTT CACCAACTAC 
AGCATCCTCA TTGGGACCAA CAAATTCTAT GAGATCGACA TGAAGCAGTA CACGCTTGAC 
GAGTTCCTGG ACAAGAACGA CCATTCCTTG GCACCTGCTG TGTTCGCCTC CTCGTCCAAC 
AGCTTCCCTG TCTCCATTGT GCAGGCGAAC AGCGCCGGGC AGCGAGAAGA ATACCTGCTG 
TGCTTCCACG AATTTGGGGT GTTCGTGGAT TCTTACGGAA GACGTAGCCG CACAGATGAT 
CTCAAGTGGA GTCGCTTACC TCTGGCCTTC GCCTACAGAG AACCTTATCT GTTTGTGACT 
CACTTCAACT CCCTGGAAGT CATTGAGATC CAGGCCAGAT CCTCACTGGG GAGCCCTGCC 
CGAGCATATC TGGAAATTCC AAACCCTCGC TACCTGGGCC CCGCGATTTC CTCCGGAGCG 
ATTTACCTGG CCTCCTCATA CCAGGACAAG TTAAGGGTCA TATGCTGCAA AGGAAACCTC 
GTGAAGGAGT CAGGCACTGA GCAGCACCGG GTGCCCTCCA CCTCCCGCAG CAGCCCCAAC 
AAGCGAGGCC CACCAACATA CAACGAGCAC ATCACCAAAC GCGTGGCCTC CAGCCCGGCG 
CCACCGGAAG GCCCCAGCCA CCCCCGAGAG CCAAGCACAC CGCACCGCTA CCGAGACAGA 
GAGGGTCGGA CAGAGCTGCG CAGGGACAAG TCTCCAGGCC GCCCTCTGGA GCGGGAGAAG 
TCCCCAGGCC GAATGCTCAG CACTAGGAGA GAGCGGTCCC CAGGGAGACT GTTTGAAGAC 
AGCAGCAGGG GCCGGCTGCC TGCAGGAGCA GTGAGGACCC CACTGTCCCA GGTTAACAAG 
GTCTGGGACC AGTCTTCAGT ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017176132.1
CDS	1419..6302
Translation	MLLGEEAMMEQEMTRLHRRVSEVEAVLSQKEVELKASETQRSLLEQDLATYITECSSLKRSLEQARMEVSQEDDKALQLLHDIREQSRKLQEIKEQEYQAQVEEMRLMMNQLEEDLVSARRRSDLYESELRESRLAAEEFKRKANECQHKLMKAKDQGKPEVGEYSKLEKINAEQQLKIQELQEKLEKAVKASTEATELLQNIRQAKERAERELEKLHNREDSSEGIKKKLVEAEERRHSLENKVKRLETMERRENRLKDDIQTKSEQIQQMADKILELEEKHREAQVSAQHLEVHLKQKEQHYEEKIKVLDNQIKKDLADKESLENMMQRHEEEAHEKGKILSEQKAMINAMDSKIRSLEQRIVELSEANKLAANSSLFTQRNMKAQEEMISELRQQKFYLETQAGKLEAQNRKLEEQLEKISHQDHSDKSRLLELETRLREVSLEHEEQKLELKRQLTELQLSLQERESQLTALQAARAALESQLRQAKTELEETTAEAEEEIQALTAHRDEIQRKFDALRNSCTVITDLEEQLNQLTEDNAELNNQNFYLSKQLDEASGANDEIVQLRSEVDHLRREITEREMQLTSQKQTMEALKTTCTMLEEQVLDLEALNDELLEKERQWEAWRSVLGDEKSQFECRVRELQRMLDTEKQSRARADQRITESRQVVELAVKEHKAEILALQQALKEQKLKAESLSDKLNDLEKKHAMLEMNARSLQQKLETERELKQRLLEEQAKLQQQMDLQKNHIFRLTQGLQEALDRADLLKTERSDLEYQLENIQVLYSHEKVKMEGTISQQTKLIDFLQAKMDQPAKKKKGLFSRRKEDPALPTQVPLQYNELKLALEKEKARCAELEEALQKTRIELRSAREEAAHRKATDHPHPSTPATARQQIAMSAIVRSPEHQPSAMSLLAPPSSRRKESSTPEEFSRRLKERMHHNIPHRFNVGLNMRATKCAVCLDTVHFGRQASKCLECQVMCHPKCSTCLPATCGLPAEYATHFTEAFCRDKMNSPGLQSKEPGSSLHLEGWMKVPRNNKRGQQGWDRKYIVLEGSKVLIYDNEAREAGQRPVEEFELCLPDGDVSIHGAVGASELANTAKADVPYILKMESHPHTTCWPGRTLYLLAPSFPDKQRWVTALESVVAGGRVSREKAEADAARDHTSSEHQPVWVEKLLGNSLLKLEGDDRLDMNCTLPFSDQVVLVGTEEGLYALNVLKNSLTHIPGIGAVFQIYIIKDLEKLLMIAGEERALCLVDVKKVKQSLAQSHLPAQPDVSPNIFEAVKGCHLFAAGKIENSLCICAAMPSKVVILRYNDNLSKYCIRKEIETSEPCSCIHFTNYSILIGTNKFYEIDMKQYTLDEFLDKNDHSLAPAVFASSSNSFPVSIVQANSAGQREEYLLCFHEFGVFVDSYGRRSRTDDLKWSRLPLAFAYREPYLFVTHFNSLEVIEIQARSSLGSPARAYLEIPNPRYLGPAISSGAIYLASSYQDKLRVICCKGNLVKESGTEQHRVPSTSRSSPNKRGPPTYNEHITKRVASSPAPPEGPSHPREPSTPHRYRDREGRTELRRDKSPGRPLEREKSPGRMLSTRRERSPGRLFEDSSRGRLPAGAVRTPLSQVNKVWDQSSV

Target ORF information:

RefSeq Version	XM_017320643.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus citron (Cit), transcript variant X25, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017320643.1

ORF Insert Sequence:

ATGCTCCTGG GCGAAGAAGC AATGATGGAG CAGGAAATGA CCCGGTTACA TCGCAGAGTG 
TCAGAGGTGG AGGCTGTGCT TAGTCAGAAG GAGGTGGAGC TGAAGGCCTC TGAGACTCAG 
AGATCCCTCC TGGAGCAGGA CCTTGCTACC TACATCACAG AATGCAGTAG CTTAAAGCGA 
AGTTTGGAGC AAGCGCGGAT GGAGGTGTCC CAGGAGGATG ACAAAGCTCT GCAGCTTCTC 
CACGACATCC GAGAGCAGAG CCGGAAGCTC CAGGAGATCA AGGAGCAGGA GTACCAGGCT 
CAGGTGGAGG AGATGAGGCT GATGATGAAT CAGCTGGAAG AAGACCTGGT GTCAGCCCGC 
AGACGCAGCG ATCTCTACGA GTCTGAGCTG AGGGAGTCTC GGCTTGCCGC CGAGGAATTC 
AAGCGGAAGG CAAACGAATG TCAGCACAAA CTGATGAAGG CTAAGGACCA AGGGAAGCCT 
GAAGTGGGAG AATATTCCAA ACTGGAGAAG ATCAATGCTG AGCAGCAGCT GAAGATCCAG 
GAGCTCCAGG AGAAGCTGGA GAAGGCTGTA AAAGCCAGCA CAGAGGCCAC CGAGCTCCTG 
CAGAATATCC GCCAGGCAAA GGAGCGAGCA GAGCGGGAGC TAGAGAAGCT ACACAACCGG 
GAAGACTCCT CCGAGGGCAT CAAAAAGAAG CTGGTGGAAG CCGAGGAACG CCGCCACTCC 
CTGGAGAACA AGGTAAAGAG ACTAGAGACC ATGGAGCGTA GAGAGAACAG ACTGAAGGAT 
GACATCCAGA CAAAGTCCGA ACAGATCCAG CAGATGGCTG ATAAAATTCT GGAGCTGGAG 
GAGAAACATC GGGAGGCTCA GGTCTCAGCT CAACATCTAG AAGTACACTT GAAACAGAAA 
GAACAGCACT ACGAGGAAAA GATCAAGGTA TTGGACAATC AGATAAAGAA GGACCTGGCC 
GACAAGGAGA GCCTGGAGAA CATGATGCAG AGACACGAGG AGGAGGCCCA CGAGAAGGGC 
AAGATCCTCA GCGAGCAGAA GGCGATGATC AACGCGATGG ATTCCAAGAT CCGATCCCTG 
GAGCAGAGGA TCGTGGAGCT GTCGGAAGCC AACAAGCTTG CGGCAAACAG CAGTCTCTTC 
ACCCAGAGGA ACATGAAGGC CCAGGAAGAG ATGATCTCAG AACTCAGGCA GCAGAAATTT 
TACCTGGAGA CGCAGGCCGG AAAGCTGGAG GCCCAGAACC GAAAGCTGGA AGAGCAACTG 
GAGAAGATCA GCCACCAAGA TCACAGTGAC AAGAGTCGGC TGCTGGAGCT GGAAACAAGG 
CTGAGGGAGG TCAGCCTGGA GCACGAGGAG CAGAAGCTGG AGCTGAAGCG GCAGCTCACG 
GAGCTGCAGC TGTCCCTGCA GGAGCGCGAG TCCCAGCTGA CGGCCCTGCA GGCCGCCCGG 
GCAGCGCTGG AGAGCCAGCT CCGCCAGGCG AAGACAGAGC TGGAGGAGAC AACCGCGGAA 
GCGGAGGAGG AGATCCAGGC GCTCACGGCA CATCGAGATG AGATCCAGCG CAAATTCGAT 
GCCCTTCGCA ACAGCTGCAC GGTCATCACC GACCTGGAGG AGCAGCTGAA CCAGCTCACC 
GAGGACAACG CCGAGCTCAA CAACCAAAAC TTCTACCTGT CCAAACAACT CGATGAGGCT 
TCCGGGGCCA ATGACGAGAT TGTGCAGCTG CGGAGTGAGG TGGACCATCT CCGCCGTGAG 
ATCACGGAGC GGGAGATGCA GCTCACCAGC CAGAAGCAAA CGATGGAGGC TCTGAAGACG 
ACATGCACCA TGCTGGAGGA GCAGGTCCTG GACCTGGAGG CCCTGAACGA CGAGCTGCTG 
GAGAAGGAGC GCCAGTGGGA GGCCTGGCGG AGCGTCCTCG GCGACGAGAA GTCCCAGTTC 
GAGTGTCGAG TTCGAGAGCT ACAGAGGATG CTGGACACCG AGAAGCAGAG CAGGGCTAGG 
GCCGATCAGC GGATCACCGA GTCGCGCCAG GTGGTGGAGT TGGCGGTGAA GGAACACAAG 
GCCGAGATTC TTGCTCTGCA GCAGGCTCTC AAGGAGCAGA AGCTCAAAGC CGAGAGCCTG 
TCGGACAAGC TCAACGACCT GGAGAAGAAG CACGCCATGC TGGAGATGAA CGCCCGGAGC 
TTACAGCAGA AACTAGAGAC AGAGCGGGAG CTCAAACAGA GGCTTCTGGA GGAGCAAGCC 
AAATTGCAGC AGCAGATGGA CCTGCAGAAG AACCACATCT TCAGACTGAC GCAAGGGCTG 
CAGGAGGCGC TGGACCGGGC GGATCTGCTG AAGACAGAAA GGAGCGACCT GGAGTACCAG 
CTGGAAAACA TTCAGGTTCT CTACTCTCAC GAGAAAGTGA AAATGGAAGG CACAATCTCT 
CAGCAAACCA AACTCATTGA TTTCCTGCAA GCCAAAATGG ACCAGCCTGC TAAAAAGAAA 
AAGGGTTTAT TTAGTCGACG GAAAGAGGAC CCTGCTTTGC CCACACAGGT TCCTCTGCAG 
TACAATGAGC TGAAGCTAGC CCTGGAAAAG GAGAAAGCCC GATGCGCGGA GCTGGAGGAG 
GCCCTTCAGA AGACCCGCAT CGAACTCCGC TCTGCGCGGG AGGAAGCTGC CCACCGCAAA 
GCCACGGACC ACCCGCACCC GTCTACGCCA GCCACTGCGA GGCAGCAGAT CGCCATGTCT 
GCCATTGTGC GGTCGCCCGA GCACCAGCCC AGTGCCATGA GCCTGCTTGC CCCACCATCC 
AGCCGCAGAA AGGAGTCGTC AACTCCAGAG GAATTCAGCC GGCGTCTGAA AGAGCGCATG 
CACCACAACA TCCCTCACCG GTTTAATGTG GGCCTGAACA TGAGAGCCAC CAAGTGCGCC 
GTGTGTCTGG ATACTGTGCA CTTTGGACGC CAGGCATCCA AATGCCTCGA ATGTCAAGTG 
ATGTGTCACC CCAAATGCTC CACCTGCTTG CCCGCTACCT GTGGCCTGCC AGCTGAATAT 
GCCACACACT TCACTGAGGC CTTCTGCCGG GACAAAATGA ACTCCCCGGG GCTCCAGAGC 
AAGGAGCCTG GCAGCAGCTT GCACCTGGAA GGGTGGATGA AAGTGCCCAG AAATAACAAA 
CGGGGACAGC AAGGCTGGGA CAGGAAGTAC ATTGTTCTGG AGGGGTCAAA AGTCCTCATC 
TATGACAATG AAGCCAGAGA AGCTGGACAG AGGCCGGTGG AAGAATTTGA GCTGTGCCTT 
CCTGACGGGG ACGTATCTAT TCATGGTGCC GTTGGTGCTT CAGAACTTGC AAATACCGCC 
AAAGCAGATG TCCCATACAT CCTGAAGATG GAGTCTCACC CACACACCAC CTGCTGGCCT 
GGGAGAACCC TCTACTTGCT AGCACCCAGC TTCCCCGACA AGCAGCGCTG GGTCACCGCC 
TTAGAATCTG TCGTCGCAGG TGGGAGAGTT TCTAGGGAAA AGGCCGAAGC CGATGCTGCA 
CGTGACCATA CTTCGAGTGA GCATCAGCCA GTGTGGGTTG AGAAATTACT TGGAAACTCT 
CTGCTGAAAC TGGAGGGCGA TGACCGGCTT GACATGAACT GCACCCTGCC CTTCAGTGAC 
CAGGTAGTGC TGGTGGGCAC GGAGGAAGGC CTCTACGCCC TGAATGTCTT GAAAAACTCC 
TTAACCCACA TCCCAGGGAT TGGCGCAGTC TTCCAAATTT ACATCATCAA GGACCTGGAG 
AAGCTGCTCA TGATAGCAGG GGAAGAGCGG GCTCTGTGCC TGGTGGACGT GAAGAAGGTG 
AAGCAGTCCC TGGCGCAGTC ACACCTGCCT GCCCAGCCCG ACGTCTCCCC CAACATATTC 
GAAGCCGTCA AAGGCTGCCA CTTGTTCGCT GCTGGCAAGA TCGAGAACAG CCTGTGCATC 
TGCGCCGCTA TGCCAAGCAA AGTCGTCATC CTCCGCTACA ATGACAACCT CAGCAAGTAC 
TGCATCCGCA AGGAGATCGA GACCTCAGAG CCCTGCAGCT GTATCCACTT CACCAACTAC 
AGCATCCTCA TTGGGACCAA CAAATTCTAT GAGATCGACA TGAAGCAGTA CACGCTTGAC 
GAGTTCCTGG ACAAGAACGA CCATTCCTTG GCACCTGCTG TGTTCGCCTC CTCGTCCAAC 
AGCTTCCCTG TCTCCATTGT GCAGGCGAAC AGCGCCGGGC AGCGAGAAGA ATACCTGCTG 
TGCTTCCACG AATTTGGGGT GTTCGTGGAT TCTTACGGAA GACGTAGCCG CACAGATGAT 
CTCAAGTGGA GTCGCTTACC TCTGGCCTTC GCCTACAGAG AACCTTATCT GTTTGTGACT 
CACTTCAACT CCCTGGAAGT CATTGAGATC CAGGCCAGAT CCTCACTGGG GAGCCCTGCC 
CGAGCATATC TGGAAATTCC AAACCCTCGC TACCTGGGCC CCGCGATTTC CTCCGGAGCG 
ATTTACCTGG CCTCCTCATA CCAGGACAAG TTAAGGGTCA TATGCTGCAA AGGAAACCTC 
GTGAAGGAGT CAGGCACTGA GCAGCACCGG GTGCCCTCCA CCTCCCGCAG CAGCCCCAAC 
AAGCGAGGCC CACCAACATA CAACGAGCAC ATCACCAAAC GCGTGGCCTC CAGCCCGGCG 
CCACCGGAAG GCCCCAGCCA CCCCCGAGAG CCAAGCACAC CGCACCGCTA CCGAGACAGA 
GAGGGTCGGA CAGAGCTGCG CAGGGACAAG TCTCCAGGCC GCCCTCTGGA GCGGGAGAAG 
TCCCCAGGCC GAATGCTCAG CACTAGGAGA GAGCGGTCCC CAGGGAGACT GTTTGAAGAC 
AGCAGCAGGG GCCGGCTGCC TGCAGGAGCA GTGAGGACCC CACTGTCCCA GGTTAACAAG 
GTCTGGGACC AGTCTTCAGT ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cit ( XM_017320644.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017176133.1 Mus musculus citron (Cit), transcript variant X28, mRNA.

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CloneID	OMu81235
Clone ID Related Accession (Same CDS sequence)	XM_017320644.2 , XM_017320644.1
Accession Version	XM_017320644.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4872bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	citron Rho-interacting kinase isoform X23
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000071.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_017320644.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTCCTGG GCGAAGAAGC AATGATGGAG CAGGAAATGA CCCGGTTACA TCGCAGAGTG 
TCAGAGGTGG AGGCTGTGCT TAGTCAGAAG GAGGTGGAGC TGAAGGCCTC TGAGACTCAG 
AGATCCCTCC TGGAGCAGGA CCTTGCTACC TACATCACAG AATGCAGTAG CTTAAAGCGA 
AGTTTGGAGC AAGCGCGGAT GGAGGTGTCC CAGGAGGATG ACAAAGCTCT GCAGCTTCTC 
CACGACATCC GAGAGCAGAG CCGGAAGCTC CAGGAGATCA AGGAGCAGGA GTACCAGGCT 
CAGGTGGAGG AGATGAGGCT GATGATGAAT CAGCTGGAAG AAGACCTGGT GTCAGCCCGC 
AGACGCAGCG ATCTCTACGA GTCTGAGCTG AGGGAGTCTC GGCTTGCCGC CGAGGAATTC 
AAGCGGAAGG CAAACGAATG TCAGCACAAA CTGATGAAGG TAGTCAGCCA CCCTCCAAGA 
GGGGACTCCG GGGGCACGGC CCTGGATGAT CTTCACAAGA TGCAAGGCCA CGCTGGCTTA 
ACGAGTGCTA AGGACCAAGG GAAGCCTGAA GTGGGAGAAT ATTCCAAACT GGAGAAGATC 
AATGCTGAGC AGCAGCTGAA GATCCAGGAG CTCCAGGAGA AGCTGGAGAA GGCTGTAAAA 
GCCAGCACAG AGGCCACCGA GCTCCTGCAG AATATCCGCC AGGCAAAGGA GCGAGCAGAG 
CGGGAGCTAG AGAAGCTACA CAACCGGGAA GACTCCTCCG AGGGCATCAA AAAGAAGCTG 
GTGGAAGCCG AGGAGCTGGA GGAGAAACAT CGGGAGGCTC AGGTCTCAGC TCAACATCTA 
GAAGTACACT TGAAACAGAA AGAACAGCAC TACGAGGAAA AGATCAAGGT ATTGGACAAT 
CAGATAAAGA AGGACCTGGC CGACAAGGAG AGCCTGGAGA ACATGATGCA GAGACACGAG 
GAGGAGGCCC ACGAGAAGGG CAAGATCCTC AGCGAGCAGA AGGCGATGAT CAACGCGATG 
GATTCCAAGA TCCGATCCCT GGAGCAGAGG ATCGTGGAGC TGTCGGAAGC CAACAAGCTT 
GCGGCAAACA GCAGTCTCTT CACCCAGAGG AACATGAAGG CCCAGGAAGA GATGATCTCA 
GAACTCAGGC AGCAGAAATT TTACCTGGAG ACGCAGGCCG GAAAGCTGGA GGCCCAGAAC 
CGAAAGCTGG AAGAGCAACT GGAGAAGATC AGCCACCAAG ATCACAGTGA CAAGAGTCGG 
CTGCTGGAGC TGGAAACAAG GCTGAGGGAG GTCAGCCTGG AGCACGAGGA GCAGAAGCTG 
GAGCTGAAGC GGCAGCTCAC GGAGCTGCAG CTGTCCCTGC AGGAGCGCGA GTCCCAGCTG 
ACGGCCCTGC AGGCCGCCCG GGCAGCGCTG GAGAGCCAGC TCCGCCAGGC GAAGACAGAG 
CTGGAGGAGA CAACCGCGGA AGCGGAGGAG GAGATCCAGG CGCTCACGGC ACATCGAGAT 
GAGATCCAGC GCAAATTCGA TGCCCTTCGC AACAGCTGCA CGGTCATCAC CGACCTGGAG 
GAGCAGCTGA ACCAGCTCAC CGAGGACAAC GCCGAGCTCA ACAACCAAAA CTTCTACCTG 
TCCAAACAAC TCGATGAGGC TTCCGGGGCC AATGACGAGA TTGTGCAGCT GCGGAGTGAG 
GTGGACCATC TCCGCCGTGA GATCACGGAG CGGGAGATGC AGCTCACCAG CCAGAAGCAA 
GCACAACTCA GTGCTCCTGA TTTGCAGACG ATGGAGGCTC TGAAGACGAC ATGCACCATG 
CTGGAGGAGC AGGTCCTGGA CCTGGAGGCC CTGAACGACG AGCTGCTGGA GAAGGAGCGC 
CAGTGGGAGG CCTGGCGGAG CGTCCTCGGC GACGAGAAGT CCCAGTTCGA GTGTCGAGTT 
CGAGAGCTAC AGAGGATGCT GGACACCGAG AAGCAGAGCA GGGCTAGGGC CGATCAGCGG 
ATCACCGAGT CGCGCCAGGT GGTGGAGTTG GCGGTGAAGG AACACAAGGC CGAGATTCTT 
GCTCTGCAGC AGGCTCTCAA GGAGCAGAAG CTCAAAGCCG AGAGCCTGTC GGACAAGCTC 
AACGACCTGG AGAAGAAGCA CGCCATGCTG GAGATGAACG CCCGGAGCTT ACAGCAGAAA 
CTAGAGACAG AGCGGGAGCT CAAACAGAGG CTTCTGGAGG AGCAAGCCAA ATTGCAGCAG 
CAGATGGACC TGCAGAAGAA CCACATCTTC AGACTGACGC AAGGGCTGCA GGAGGCGCTG 
GACCGGGCGG ATCTGCTGAA GACAGAAAGG AGCGACCTGG AGTACCAGCT GGAAAACATT 
CAGGTTCTCT ACTCTCACGA GAAAGTGAAA ATGGAAGGCA CAATCTCTCA GCAAACCAAA 
CTCATTGATT TCCTGCAAGC CAAAATGGAC CAGCCTGCTA AAAAGAAAAA GGGTTTATTT 
AGTCGACGGA AAGAGGACCC TGCTTTGCCC ACACAGGTTC CTCTGCAGTA CAATGAGCTG 
AAGCTAGCCC TGGAAAAGGA GAAAGCCCGA TGCGCGGAGC TGGAGGAGGC CCTTCAGAAG 
ACCCGCATCG AACTCCGCTC TGCGCGGGAG GAAGCTGCCC ACCGCAAAGC CACGGACCAC 
CCGCACCCGT CTACGCCAGC CACTGCGAGG CAGCAGATCG CCATGTCTGC CATTGTGCGG 
TCGCCCGAGC ACCAGCCCAG TGCCATGAGC CTGCTTGCCC CACCATCCAG CCGCAGAAAG 
GAGTCGTCAA CTCCAGAGGA ATTCAGCCGG CGTCTGAAAG AGCGCATGCA CCACAACATC 
CCTCACCGGT TTAATGTGGG CCTGAACATG AGAGCCACCA AGTGCGCCGT GTGTCTGGAT 
ACTGTGCACT TTGGACGCCA GGCATCCAAA TGCCTCGAAT GTCAAGTGAT GTGTCACCCC 
AAATGCTCCA CCTGCTTGCC CGCTACCTGT GGCCTGCCAG CTGAATATGC CACACACTTC 
ACTGAGGCCT TCTGCCGGGA CAAAATGAAC TCCCCGGGGC TCCAGAGCAA GGAGCCTGGC 
AGCAGCTTGC ACCTGGAAGG GTGGATGAAA GTGCCCAGAA ATAACAAACG GGGACAGCAA 
GGCTGGGACA GGAAGTACAT TGTTCTGGAG GGGTCAAAAG TCCTCATCTA TGACAATGAA 
GCCAGAGAAG CTGGACAGAG GCCGGTGGAA GAATTTGAGC TGTGCCTTCC TGACGGGGAC 
GTATCTATTC ATGGTGCCGT TGGTGCTTCA GAACTTGCAA ATACCGCCAA AGCAGATGTC 
CCATACATCC TGAAGATGGA GTCTCACCCA CACACCACCT GCTGGCCTGG GAGAACCCTC 
TACTTGCTAG CACCCAGCTT CCCCGACAAG CAGCGCTGGG TCACCGCCTT AGAATCTGTC 
GTCGCAGGTG GGAGAGTTTC TAGGGAAAAG GCCGAAGCCG ATGCTGCACG TGACCATACT 
TCGAGTGAGC ATCAGCCAGT GTGGGTTGAG AAATTACTTG GAAACTCTCT GCTGAAACTG 
GAGGGCGATG ACCGGCTTGA CATGAACTGC ACCCTGCCCT TCAGTGACCA GGTAGTGCTG 
GTGGGCACGG AGGAAGGCCT CTACGCCCTG AATGTCTTGA AAAACTCCTT AACCCACATC 
CCAGGGATTG GCGCAGTCTT CCAAATTTAC ATCATCAAGG ACCTGGAGAA GCTGCTCATG 
ATAGCAGGGG AAGAGCGGGC TCTGTGCCTG GTGGACGTGA AGAAGGTGAA GCAGTCCCTG 
GCGCAGTCAC ACCTGCCTGC CCAGCCCGAC GTCTCCCCCA ACATATTCGA AGCCGTCAAA 
GGCTGCCACT TGTTCGCTGC TGGCAAGATC GAGAACAGCC TGTGCATCTG CGCCGCTATG 
CCAAGCAAAG TCGTCATCCT CCGCTACAAT GACAACCTCA GCAAGTACTG CATCCGCAAG 
GAGATCGAGA CCTCAGAGCC CTGCAGCTGT ATCCACTTCA CCAACTACAG CATCCTCATT 
GGGACCAACA AATTCTATGA GATCGACATG AAGCAGTACA CGCTTGACGA GTTCCTGGAC 
AAGAACGACC ATTCCTTGGC ACCTGCTGTG TTCGCCTCCT CGTCCAACAG CTTCCCTGTC 
TCCATTGTGC AGGCGAACAG CGCCGGGCAG CGAGAAGAAT ACCTGCTGTG CTTCCACGAA 
TTTGGGGTGT TCGTGGATTC TTACGGAAGA CGTAGCCGCA CAGATGATCT CAAGTGGAGT 
CGCTTACCTC TGGCCTTCGC CTACAGAGAA CCTTATCTGT TTGTGACTCA CTTCAACTCC 
CTGGAAGTCA TTGAGATCCA GGCCAGATCC TCACTGGGGA GCCCTGCCCG AGCATATCTG 
GAAATTCCAA ACCCTCGCTA CCTGGGCCCC GCGATTTCCT CCGGAGCGAT TTACCTGGCC 
TCCTCATACC AGGACAAGTT AAGGGTCATA TGCTGCAAAG GAAACCTCGT GAAGGAGTCA 
GGCACTGAGC AGCACCGGGT GCCCTCCACC TCCCGCAGCA GCCCCAACAA GCGAGGCCCA 
CCAACATACA ACGAGCACAT CACCAAACGC GTGGCCTCCA GCCCGGCGCC ACCGGAAGGC 
CCCAGCCACC CCCGAGAGCC AAGCACACCG CACCGCTACC GAGACAGAGA GGGTCGGACA 
GAGCTGCGCA GGGACAAGTC TCCAGGCCGC CCTCTGGAGC GGGAGAAGTC CCCAGGCCGA 
ATGCTCAGCA CTAGGAGAGA GCGGTCCCCA GGGAGACTGT TTGAAGACAG CAGCAGGGGC 
CGGCTGCCTG CAGGAGCAGT GAGGACCCCA CTGTCCCAGG TTAACAAGGT CTGGGACCAG 
TCTTCAGTAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017176133.1
CDS	1147..6018
Translation	MLLGEEAMMEQEMTRLHRRVSEVEAVLSQKEVELKASETQRSLLEQDLATYITECSSLKRSLEQARMEVSQEDDKALQLLHDIREQSRKLQEIKEQEYQAQVEEMRLMMNQLEEDLVSARRRSDLYESELRESRLAAEEFKRKANECQHKLMKVVSHPPRGDSGGTALDDLHKMQGHAGLTSAKDQGKPEVGEYSKLEKINAEQQLKIQELQEKLEKAVKASTEATELLQNIRQAKERAERELEKLHNREDSSEGIKKKLVEAEELEEKHREAQVSAQHLEVHLKQKEQHYEEKIKVLDNQIKKDLADKESLENMMQRHEEEAHEKGKILSEQKAMINAMDSKIRSLEQRIVELSEANKLAANSSLFTQRNMKAQEEMISELRQQKFYLETQAGKLEAQNRKLEEQLEKISHQDHSDKSRLLELETRLREVSLEHEEQKLELKRQLTELQLSLQERESQLTALQAARAALESQLRQAKTELEETTAEAEEEIQALTAHRDEIQRKFDALRNSCTVITDLEEQLNQLTEDNAELNNQNFYLSKQLDEASGANDEIVQLRSEVDHLRREITEREMQLTSQKQAQLSAPDLQTMEALKTTCTMLEEQVLDLEALNDELLEKERQWEAWRSVLGDEKSQFECRVRELQRMLDTEKQSRARADQRITESRQVVELAVKEHKAEILALQQALKEQKLKAESLSDKLNDLEKKHAMLEMNARSLQQKLETERELKQRLLEEQAKLQQQMDLQKNHIFRLTQGLQEALDRADLLKTERSDLEYQLENIQVLYSHEKVKMEGTISQQTKLIDFLQAKMDQPAKKKKGLFSRRKEDPALPTQVPLQYNELKLALEKEKARCAELEEALQKTRIELRSAREEAAHRKATDHPHPSTPATARQQIAMSAIVRSPEHQPSAMSLLAPPSSRRKESSTPEEFSRRLKERMHHNIPHRFNVGLNMRATKCAVCLDTVHFGRQASKCLECQVMCHPKCSTCLPATCGLPAEYATHFTEAFCRDKMNSPGLQSKEPGSSLHLEGWMKVPRNNKRGQQGWDRKYIVLEGSKVLIYDNEAREAGQRPVEEFELCLPDGDVSIHGAVGASELANTAKADVPYILKMESHPHTTCWPGRTLYLLAPSFPDKQRWVTALESVVAGGRVSREKAEADAARDHTSSEHQPVWVEKLLGNSLLKLEGDDRLDMNCTLPFSDQVVLVGTEEGLYALNVLKNSLTHIPGIGAVFQIYIIKDLEKLLMIAGEERALCLVDVKKVKQSLAQSHLPAQPDVSPNIFEAVKGCHLFAAGKIENSLCICAAMPSKVVILRYNDNLSKYCIRKEIETSEPCSCIHFTNYSILIGTNKFYEIDMKQYTLDEFLDKNDHSLAPAVFASSSNSFPVSIVQANSAGQREEYLLCFHEFGVFVDSYGRRSRTDDLKWSRLPLAFAYREPYLFVTHFNSLEVIEIQARSSLGSPARAYLEIPNPRYLGPAISSGAIYLASSYQDKLRVICCKGNLVKESGTEQHRVPSTSRSSPNKRGPPTYNEHITKRVASSPAPPEGPSHPREPSTPHRYRDREGRTELRRDKSPGRPLEREKSPGRMLSTRRERSPGRLFEDSSRGRLPAGAVRTPLSQVNKVWDQSSV

Target ORF information:

RefSeq Version	XM_017320644.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus citron (Cit), transcript variant X28, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017320644.2

ORF Insert Sequence:

ATGCTCCTGG GCGAAGAAGC AATGATGGAG CAGGAAATGA CCCGGTTACA TCGCAGAGTG 
TCAGAGGTGG AGGCTGTGCT TAGTCAGAAG GAGGTGGAGC TGAAGGCCTC TGAGACTCAG 
AGATCCCTCC TGGAGCAGGA CCTTGCTACC TACATCACAG AATGCAGTAG CTTAAAGCGA 
AGTTTGGAGC AAGCGCGGAT GGAGGTGTCC CAGGAGGATG ACAAAGCTCT GCAGCTTCTC 
CACGACATCC GAGAGCAGAG CCGGAAGCTC CAGGAGATCA AGGAGCAGGA GTACCAGGCT 
CAGGTGGAGG AGATGAGGCT GATGATGAAT CAGCTGGAAG AAGACCTGGT GTCAGCCCGC 
AGACGCAGCG ATCTCTACGA GTCTGAGCTG AGGGAGTCTC GGCTTGCCGC CGAGGAATTC 
AAGCGGAAGG CAAACGAATG TCAGCACAAA CTGATGAAGG TAGTCAGCCA CCCTCCAAGA 
GGGGACTCCG GGGGCACGGC CCTGGATGAT CTTCACAAGA TGCAAGGCCA CGCTGGCTTA 
ACGAGTGCTA AGGACCAAGG GAAGCCTGAA GTGGGAGAAT ATTCCAAACT GGAGAAGATC 
AATGCTGAGC AGCAGCTGAA GATCCAGGAG CTCCAGGAGA AGCTGGAGAA GGCTGTAAAA 
GCCAGCACAG AGGCCACCGA GCTCCTGCAG AATATCCGCC AGGCAAAGGA GCGAGCAGAG 
CGGGAGCTAG AGAAGCTACA CAACCGGGAA GACTCCTCCG AGGGCATCAA AAAGAAGCTG 
GTGGAAGCCG AGGAGCTGGA GGAGAAACAT CGGGAGGCTC AGGTCTCAGC TCAACATCTA 
GAAGTACACT TGAAACAGAA AGAACAGCAC TACGAGGAAA AGATCAAGGT ATTGGACAAT 
CAGATAAAGA AGGACCTGGC CGACAAGGAG AGCCTGGAGA ACATGATGCA GAGACACGAG 
GAGGAGGCCC ACGAGAAGGG CAAGATCCTC AGCGAGCAGA AGGCGATGAT CAACGCGATG 
GATTCCAAGA TCCGATCCCT GGAGCAGAGG ATCGTGGAGC TGTCGGAAGC CAACAAGCTT 
GCGGCAAACA GCAGTCTCTT CACCCAGAGG AACATGAAGG CCCAGGAAGA GATGATCTCA 
GAACTCAGGC AGCAGAAATT TTACCTGGAG ACGCAGGCCG GAAAGCTGGA GGCCCAGAAC 
CGAAAGCTGG AAGAGCAACT GGAGAAGATC AGCCACCAAG ATCACAGTGA CAAGAGTCGG 
CTGCTGGAGC TGGAAACAAG GCTGAGGGAG GTCAGCCTGG AGCACGAGGA GCAGAAGCTG 
GAGCTGAAGC GGCAGCTCAC GGAGCTGCAG CTGTCCCTGC AGGAGCGCGA GTCCCAGCTG 
ACGGCCCTGC AGGCCGCCCG GGCAGCGCTG GAGAGCCAGC TCCGCCAGGC GAAGACAGAG 
CTGGAGGAGA CAACCGCGGA AGCGGAGGAG GAGATCCAGG CGCTCACGGC ACATCGAGAT 
GAGATCCAGC GCAAATTCGA TGCCCTTCGC AACAGCTGCA CGGTCATCAC CGACCTGGAG 
GAGCAGCTGA ACCAGCTCAC CGAGGACAAC GCCGAGCTCA ACAACCAAAA CTTCTACCTG 
TCCAAACAAC TCGATGAGGC TTCCGGGGCC AATGACGAGA TTGTGCAGCT GCGGAGTGAG 
GTGGACCATC TCCGCCGTGA GATCACGGAG CGGGAGATGC AGCTCACCAG CCAGAAGCAA 
GCACAACTCA GTGCTCCTGA TTTGCAGACG ATGGAGGCTC TGAAGACGAC ATGCACCATG 
CTGGAGGAGC AGGTCCTGGA CCTGGAGGCC CTGAACGACG AGCTGCTGGA GAAGGAGCGC 
CAGTGGGAGG CCTGGCGGAG CGTCCTCGGC GACGAGAAGT CCCAGTTCGA GTGTCGAGTT 
CGAGAGCTAC AGAGGATGCT GGACACCGAG AAGCAGAGCA GGGCTAGGGC CGATCAGCGG 
ATCACCGAGT CGCGCCAGGT GGTGGAGTTG GCGGTGAAGG AACACAAGGC CGAGATTCTT 
GCTCTGCAGC AGGCTCTCAA GGAGCAGAAG CTCAAAGCCG AGAGCCTGTC GGACAAGCTC 
AACGACCTGG AGAAGAAGCA CGCCATGCTG GAGATGAACG CCCGGAGCTT ACAGCAGAAA 
CTAGAGACAG AGCGGGAGCT CAAACAGAGG CTTCTGGAGG AGCAAGCCAA ATTGCAGCAG 
CAGATGGACC TGCAGAAGAA CCACATCTTC AGACTGACGC AAGGGCTGCA GGAGGCGCTG 
GACCGGGCGG ATCTGCTGAA GACAGAAAGG AGCGACCTGG AGTACCAGCT GGAAAACATT 
CAGGTTCTCT ACTCTCACGA GAAAGTGAAA ATGGAAGGCA CAATCTCTCA GCAAACCAAA 
CTCATTGATT TCCTGCAAGC CAAAATGGAC CAGCCTGCTA AAAAGAAAAA GGGTTTATTT 
AGTCGACGGA AAGAGGACCC TGCTTTGCCC ACACAGGTTC CTCTGCAGTA CAATGAGCTG 
AAGCTAGCCC TGGAAAAGGA GAAAGCCCGA TGCGCGGAGC TGGAGGAGGC CCTTCAGAAG 
ACCCGCATCG AACTCCGCTC TGCGCGGGAG GAAGCTGCCC ACCGCAAAGC CACGGACCAC 
CCGCACCCGT CTACGCCAGC CACTGCGAGG CAGCAGATCG CCATGTCTGC CATTGTGCGG 
TCGCCCGAGC ACCAGCCCAG TGCCATGAGC CTGCTTGCCC CACCATCCAG CCGCAGAAAG 
GAGTCGTCAA CTCCAGAGGA ATTCAGCCGG CGTCTGAAAG AGCGCATGCA CCACAACATC 
CCTCACCGGT TTAATGTGGG CCTGAACATG AGAGCCACCA AGTGCGCCGT GTGTCTGGAT 
ACTGTGCACT TTGGACGCCA GGCATCCAAA TGCCTCGAAT GTCAAGTGAT GTGTCACCCC 
AAATGCTCCA CCTGCTTGCC CGCTACCTGT GGCCTGCCAG CTGAATATGC CACACACTTC 
ACTGAGGCCT TCTGCCGGGA CAAAATGAAC TCCCCGGGGC TCCAGAGCAA GGAGCCTGGC 
AGCAGCTTGC ACCTGGAAGG GTGGATGAAA GTGCCCAGAA ATAACAAACG GGGACAGCAA 
GGCTGGGACA GGAAGTACAT TGTTCTGGAG GGGTCAAAAG TCCTCATCTA TGACAATGAA 
GCCAGAGAAG CTGGACAGAG GCCGGTGGAA GAATTTGAGC TGTGCCTTCC TGACGGGGAC 
GTATCTATTC ATGGTGCCGT TGGTGCTTCA GAACTTGCAA ATACCGCCAA AGCAGATGTC 
CCATACATCC TGAAGATGGA GTCTCACCCA CACACCACCT GCTGGCCTGG GAGAACCCTC 
TACTTGCTAG CACCCAGCTT CCCCGACAAG CAGCGCTGGG TCACCGCCTT AGAATCTGTC 
GTCGCAGGTG GGAGAGTTTC TAGGGAAAAG GCCGAAGCCG ATGCTGCACG TGACCATACT 
TCGAGTGAGC ATCAGCCAGT GTGGGTTGAG AAATTACTTG GAAACTCTCT GCTGAAACTG 
GAGGGCGATG ACCGGCTTGA CATGAACTGC ACCCTGCCCT TCAGTGACCA GGTAGTGCTG 
GTGGGCACGG AGGAAGGCCT CTACGCCCTG AATGTCTTGA AAAACTCCTT AACCCACATC 
CCAGGGATTG GCGCAGTCTT CCAAATTTAC ATCATCAAGG ACCTGGAGAA GCTGCTCATG 
ATAGCAGGGG AAGAGCGGGC TCTGTGCCTG GTGGACGTGA AGAAGGTGAA GCAGTCCCTG 
GCGCAGTCAC ACCTGCCTGC CCAGCCCGAC GTCTCCCCCA ACATATTCGA AGCCGTCAAA 
GGCTGCCACT TGTTCGCTGC TGGCAAGATC GAGAACAGCC TGTGCATCTG CGCCGCTATG 
CCAAGCAAAG TCGTCATCCT CCGCTACAAT GACAACCTCA GCAAGTACTG CATCCGCAAG 
GAGATCGAGA CCTCAGAGCC CTGCAGCTGT ATCCACTTCA CCAACTACAG CATCCTCATT 
GGGACCAACA AATTCTATGA GATCGACATG AAGCAGTACA CGCTTGACGA GTTCCTGGAC 
AAGAACGACC ATTCCTTGGC ACCTGCTGTG TTCGCCTCCT CGTCCAACAG CTTCCCTGTC 
TCCATTGTGC AGGCGAACAG CGCCGGGCAG CGAGAAGAAT ACCTGCTGTG CTTCCACGAA 
TTTGGGGTGT TCGTGGATTC TTACGGAAGA CGTAGCCGCA CAGATGATCT CAAGTGGAGT 
CGCTTACCTC TGGCCTTCGC CTACAGAGAA CCTTATCTGT TTGTGACTCA CTTCAACTCC 
CTGGAAGTCA TTGAGATCCA GGCCAGATCC TCACTGGGGA GCCCTGCCCG AGCATATCTG 
GAAATTCCAA ACCCTCGCTA CCTGGGCCCC GCGATTTCCT CCGGAGCGAT TTACCTGGCC 
TCCTCATACC AGGACAAGTT AAGGGTCATA TGCTGCAAAG GAAACCTCGT GAAGGAGTCA 
GGCACTGAGC AGCACCGGGT GCCCTCCACC TCCCGCAGCA GCCCCAACAA GCGAGGCCCA 
CCAACATACA ACGAGCACAT CACCAAACGC GTGGCCTCCA GCCCGGCGCC ACCGGAAGGC 
CCCAGCCACC CCCGAGAGCC AAGCACACCG CACCGCTACC GAGACAGAGA GGGTCGGACA 
GAGCTGCGCA GGGACAAGTC TCCAGGCCGC CCTCTGGAGC GGGAGAAGTC CCCAGGCCGA 
ATGCTCAGCA CTAGGAGAGA GCGGTCCCCA GGGAGACTGT TTGAAGACAG CAGCAGGGGC 
CGGCTGCCTG CAGGAGCAGT GAGGACCCCA CTGTCCCAGG TTAACAAGGT CTGGGACCAG 
TCTTCAGTAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cit ( XM_017320644.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017176133.1 Mus musculus citron (Cit), transcript variant X26, mRNA.

Price & Availability↑

CloneID	OMu81235
Clone ID Related Accession (Same CDS sequence)	XM_017320644.2 , XM_017320644.1
Accession Version	XM_017320644.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4872bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	citron Rho-interacting kinase isoform X22
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000071.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTCCTGG GCGAAGAAGC AATGATGGAG CAGGAAATGA CCCGGTTACA TCGCAGAGTG 
TCAGAGGTGG AGGCTGTGCT TAGTCAGAAG GAGGTGGAGC TGAAGGCCTC TGAGACTCAG 
AGATCCCTCC TGGAGCAGGA CCTTGCTACC TACATCACAG AATGCAGTAG CTTAAAGCGA 
AGTTTGGAGC AAGCGCGGAT GGAGGTGTCC CAGGAGGATG ACAAAGCTCT GCAGCTTCTC 
CACGACATCC GAGAGCAGAG CCGGAAGCTC CAGGAGATCA AGGAGCAGGA GTACCAGGCT 
CAGGTGGAGG AGATGAGGCT GATGATGAAT CAGCTGGAAG AAGACCTGGT GTCAGCCCGC 
AGACGCAGCG ATCTCTACGA GTCTGAGCTG AGGGAGTCTC GGCTTGCCGC CGAGGAATTC 
AAGCGGAAGG CAAACGAATG TCAGCACAAA CTGATGAAGG TAGTCAGCCA CCCTCCAAGA 
GGGGACTCCG GGGGCACGGC CCTGGATGAT CTTCACAAGA TGCAAGGCCA CGCTGGCTTA 
ACGAGTGCTA AGGACCAAGG GAAGCCTGAA GTGGGAGAAT ATTCCAAACT GGAGAAGATC 
AATGCTGAGC AGCAGCTGAA GATCCAGGAG CTCCAGGAGA AGCTGGAGAA GGCTGTAAAA 
GCCAGCACAG AGGCCACCGA GCTCCTGCAG AATATCCGCC AGGCAAAGGA GCGAGCAGAG 
CGGGAGCTAG AGAAGCTACA CAACCGGGAA GACTCCTCCG AGGGCATCAA AAAGAAGCTG 
GTGGAAGCCG AGGAGCTGGA GGAGAAACAT CGGGAGGCTC AGGTCTCAGC TCAACATCTA 
GAAGTACACT TGAAACAGAA AGAACAGCAC TACGAGGAAA AGATCAAGGT ATTGGACAAT 
CAGATAAAGA AGGACCTGGC CGACAAGGAG AGCCTGGAGA ACATGATGCA GAGACACGAG 
GAGGAGGCCC ACGAGAAGGG CAAGATCCTC AGCGAGCAGA AGGCGATGAT CAACGCGATG 
GATTCCAAGA TCCGATCCCT GGAGCAGAGG ATCGTGGAGC TGTCGGAAGC CAACAAGCTT 
GCGGCAAACA GCAGTCTCTT CACCCAGAGG AACATGAAGG CCCAGGAAGA GATGATCTCA 
GAACTCAGGC AGCAGAAATT TTACCTGGAG ACGCAGGCCG GAAAGCTGGA GGCCCAGAAC 
CGAAAGCTGG AAGAGCAACT GGAGAAGATC AGCCACCAAG ATCACAGTGA CAAGAGTCGG 
CTGCTGGAGC TGGAAACAAG GCTGAGGGAG GTCAGCCTGG AGCACGAGGA GCAGAAGCTG 
GAGCTGAAGC GGCAGCTCAC GGAGCTGCAG CTGTCCCTGC AGGAGCGCGA GTCCCAGCTG 
ACGGCCCTGC AGGCCGCCCG GGCAGCGCTG GAGAGCCAGC TCCGCCAGGC GAAGACAGAG 
CTGGAGGAGA CAACCGCGGA AGCGGAGGAG GAGATCCAGG CGCTCACGGC ACATCGAGAT 
GAGATCCAGC GCAAATTCGA TGCCCTTCGC AACAGCTGCA CGGTCATCAC CGACCTGGAG 
GAGCAGCTGA ACCAGCTCAC CGAGGACAAC GCCGAGCTCA ACAACCAAAA CTTCTACCTG 
TCCAAACAAC TCGATGAGGC TTCCGGGGCC AATGACGAGA TTGTGCAGCT GCGGAGTGAG 
GTGGACCATC TCCGCCGTGA GATCACGGAG CGGGAGATGC AGCTCACCAG CCAGAAGCAA 
GCACAACTCA GTGCTCCTGA TTTGCAGACG ATGGAGGCTC TGAAGACGAC ATGCACCATG 
CTGGAGGAGC AGGTCCTGGA CCTGGAGGCC CTGAACGACG AGCTGCTGGA GAAGGAGCGC 
CAGTGGGAGG CCTGGCGGAG CGTCCTCGGC GACGAGAAGT CCCAGTTCGA GTGTCGAGTT 
CGAGAGCTAC AGAGGATGCT GGACACCGAG AAGCAGAGCA GGGCTAGGGC CGATCAGCGG 
ATCACCGAGT CGCGCCAGGT GGTGGAGTTG GCGGTGAAGG AACACAAGGC CGAGATTCTT 
GCTCTGCAGC AGGCTCTCAA GGAGCAGAAG CTCAAAGCCG AGAGCCTGTC GGACAAGCTC 
AACGACCTGG AGAAGAAGCA CGCCATGCTG GAGATGAACG CCCGGAGCTT ACAGCAGAAA 
CTAGAGACAG AGCGGGAGCT CAAACAGAGG CTTCTGGAGG AGCAAGCCAA ATTGCAGCAG 
CAGATGGACC TGCAGAAGAA CCACATCTTC AGACTGACGC AAGGGCTGCA GGAGGCGCTG 
GACCGGGCGG ATCTGCTGAA GACAGAAAGG AGCGACCTGG AGTACCAGCT GGAAAACATT 
CAGGTTCTCT ACTCTCACGA GAAAGTGAAA ATGGAAGGCA CAATCTCTCA GCAAACCAAA 
CTCATTGATT TCCTGCAAGC CAAAATGGAC CAGCCTGCTA AAAAGAAAAA GGGTTTATTT 
AGTCGACGGA AAGAGGACCC TGCTTTGCCC ACACAGGTTC CTCTGCAGTA CAATGAGCTG 
AAGCTAGCCC TGGAAAAGGA GAAAGCCCGA TGCGCGGAGC TGGAGGAGGC CCTTCAGAAG 
ACCCGCATCG AACTCCGCTC TGCGCGGGAG GAAGCTGCCC ACCGCAAAGC CACGGACCAC 
CCGCACCCGT CTACGCCAGC CACTGCGAGG CAGCAGATCG CCATGTCTGC CATTGTGCGG 
TCGCCCGAGC ACCAGCCCAG TGCCATGAGC CTGCTTGCCC CACCATCCAG CCGCAGAAAG 
GAGTCGTCAA CTCCAGAGGA ATTCAGCCGG CGTCTGAAAG AGCGCATGCA CCACAACATC 
CCTCACCGGT TTAATGTGGG CCTGAACATG AGAGCCACCA AGTGCGCCGT GTGTCTGGAT 
ACTGTGCACT TTGGACGCCA GGCATCCAAA TGCCTCGAAT GTCAAGTGAT GTGTCACCCC 
AAATGCTCCA CCTGCTTGCC CGCTACCTGT GGCCTGCCAG CTGAATATGC CACACACTTC 
ACTGAGGCCT TCTGCCGGGA CAAAATGAAC TCCCCGGGGC TCCAGAGCAA GGAGCCTGGC 
AGCAGCTTGC ACCTGGAAGG GTGGATGAAA GTGCCCAGAA ATAACAAACG GGGACAGCAA 
GGCTGGGACA GGAAGTACAT TGTTCTGGAG GGGTCAAAAG TCCTCATCTA TGACAATGAA 
GCCAGAGAAG CTGGACAGAG GCCGGTGGAA GAATTTGAGC TGTGCCTTCC TGACGGGGAC 
GTATCTATTC ATGGTGCCGT TGGTGCTTCA GAACTTGCAA ATACCGCCAA AGCAGATGTC 
CCATACATCC TGAAGATGGA GTCTCACCCA CACACCACCT GCTGGCCTGG GAGAACCCTC 
TACTTGCTAG CACCCAGCTT CCCCGACAAG CAGCGCTGGG TCACCGCCTT AGAATCTGTC 
GTCGCAGGTG GGAGAGTTTC TAGGGAAAAG GCCGAAGCCG ATGCTGCACG TGACCATACT 
TCGAGTGAGC ATCAGCCAGT GTGGGTTGAG AAATTACTTG GAAACTCTCT GCTGAAACTG 
GAGGGCGATG ACCGGCTTGA CATGAACTGC ACCCTGCCCT TCAGTGACCA GGTAGTGCTG 
GTGGGCACGG AGGAAGGCCT CTACGCCCTG AATGTCTTGA AAAACTCCTT AACCCACATC 
CCAGGGATTG GCGCAGTCTT CCAAATTTAC ATCATCAAGG ACCTGGAGAA GCTGCTCATG 
ATAGCAGGGG AAGAGCGGGC TCTGTGCCTG GTGGACGTGA AGAAGGTGAA GCAGTCCCTG 
GCGCAGTCAC ACCTGCCTGC CCAGCCCGAC GTCTCCCCCA ACATATTCGA AGCCGTCAAA 
GGCTGCCACT TGTTCGCTGC TGGCAAGATC GAGAACAGCC TGTGCATCTG CGCCGCTATG 
CCAAGCAAAG TCGTCATCCT CCGCTACAAT GACAACCTCA GCAAGTACTG CATCCGCAAG 
GAGATCGAGA CCTCAGAGCC CTGCAGCTGT ATCCACTTCA CCAACTACAG CATCCTCATT 
GGGACCAACA AATTCTATGA GATCGACATG AAGCAGTACA CGCTTGACGA GTTCCTGGAC 
AAGAACGACC ATTCCTTGGC ACCTGCTGTG TTCGCCTCCT CGTCCAACAG CTTCCCTGTC 
TCCATTGTGC AGGCGAACAG CGCCGGGCAG CGAGAAGAAT ACCTGCTGTG CTTCCACGAA 
TTTGGGGTGT TCGTGGATTC TTACGGAAGA CGTAGCCGCA CAGATGATCT CAAGTGGAGT 
CGCTTACCTC TGGCCTTCGC CTACAGAGAA CCTTATCTGT TTGTGACTCA CTTCAACTCC 
CTGGAAGTCA TTGAGATCCA GGCCAGATCC TCACTGGGGA GCCCTGCCCG AGCATATCTG 
GAAATTCCAA ACCCTCGCTA CCTGGGCCCC GCGATTTCCT CCGGAGCGAT TTACCTGGCC 
TCCTCATACC AGGACAAGTT AAGGGTCATA TGCTGCAAAG GAAACCTCGT GAAGGAGTCA 
GGCACTGAGC AGCACCGGGT GCCCTCCACC TCCCGCAGCA GCCCCAACAA GCGAGGCCCA 
CCAACATACA ACGAGCACAT CACCAAACGC GTGGCCTCCA GCCCGGCGCC ACCGGAAGGC 
CCCAGCCACC CCCGAGAGCC AAGCACACCG CACCGCTACC GAGACAGAGA GGGTCGGACA 
GAGCTGCGCA GGGACAAGTC TCCAGGCCGC CCTCTGGAGC GGGAGAAGTC CCCAGGCCGA 
ATGCTCAGCA CTAGGAGAGA GCGGTCCCCA GGGAGACTGT TTGAAGACAG CAGCAGGGGC 
CGGCTGCCTG CAGGAGCAGT GAGGACCCCA CTGTCCCAGG TTAACAAGGT CTGGGACCAG 
TCTTCAGTAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017176133.1
CDS	1419..6290
Translation	MLLGEEAMMEQEMTRLHRRVSEVEAVLSQKEVELKASETQRSLLEQDLATYITECSSLKRSLEQARMEVSQEDDKALQLLHDIREQSRKLQEIKEQEYQAQVEEMRLMMNQLEEDLVSARRRSDLYESELRESRLAAEEFKRKANECQHKLMKVVSHPPRGDSGGTALDDLHKMQGHAGLTSAKDQGKPEVGEYSKLEKINAEQQLKIQELQEKLEKAVKASTEATELLQNIRQAKERAERELEKLHNREDSSEGIKKKLVEAEELEEKHREAQVSAQHLEVHLKQKEQHYEEKIKVLDNQIKKDLADKESLENMMQRHEEEAHEKGKILSEQKAMINAMDSKIRSLEQRIVELSEANKLAANSSLFTQRNMKAQEEMISELRQQKFYLETQAGKLEAQNRKLEEQLEKISHQDHSDKSRLLELETRLREVSLEHEEQKLELKRQLTELQLSLQERESQLTALQAARAALESQLRQAKTELEETTAEAEEEIQALTAHRDEIQRKFDALRNSCTVITDLEEQLNQLTEDNAELNNQNFYLSKQLDEASGANDEIVQLRSEVDHLRREITEREMQLTSQKQAQLSAPDLQTMEALKTTCTMLEEQVLDLEALNDELLEKERQWEAWRSVLGDEKSQFECRVRELQRMLDTEKQSRARADQRITESRQVVELAVKEHKAEILALQQALKEQKLKAESLSDKLNDLEKKHAMLEMNARSLQQKLETERELKQRLLEEQAKLQQQMDLQKNHIFRLTQGLQEALDRADLLKTERSDLEYQLENIQVLYSHEKVKMEGTISQQTKLIDFLQAKMDQPAKKKKGLFSRRKEDPALPTQVPLQYNELKLALEKEKARCAELEEALQKTRIELRSAREEAAHRKATDHPHPSTPATARQQIAMSAIVRSPEHQPSAMSLLAPPSSRRKESSTPEEFSRRLKERMHHNIPHRFNVGLNMRATKCAVCLDTVHFGRQASKCLECQVMCHPKCSTCLPATCGLPAEYATHFTEAFCRDKMNSPGLQSKEPGSSLHLEGWMKVPRNNKRGQQGWDRKYIVLEGSKVLIYDNEAREAGQRPVEEFELCLPDGDVSIHGAVGASELANTAKADVPYILKMESHPHTTCWPGRTLYLLAPSFPDKQRWVTALESVVAGGRVSREKAEADAARDHTSSEHQPVWVEKLLGNSLLKLEGDDRLDMNCTLPFSDQVVLVGTEEGLYALNVLKNSLTHIPGIGAVFQIYIIKDLEKLLMIAGEERALCLVDVKKVKQSLAQSHLPAQPDVSPNIFEAVKGCHLFAAGKIENSLCICAAMPSKVVILRYNDNLSKYCIRKEIETSEPCSCIHFTNYSILIGTNKFYEIDMKQYTLDEFLDKNDHSLAPAVFASSSNSFPVSIVQANSAGQREEYLLCFHEFGVFVDSYGRRSRTDDLKWSRLPLAFAYREPYLFVTHFNSLEVIEIQARSSLGSPARAYLEIPNPRYLGPAISSGAIYLASSYQDKLRVICCKGNLVKESGTEQHRVPSTSRSSPNKRGPPTYNEHITKRVASSPAPPEGPSHPREPSTPHRYRDREGRTELRRDKSPGRPLEREKSPGRMLSTRRERSPGRLFEDSSRGRLPAGAVRTPLSQVNKVWDQSSV

Target ORF information:

RefSeq Version	XM_017320644.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus citron (Cit), transcript variant X26, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017320644.1

ORF Insert Sequence:

ATGCTCCTGG GCGAAGAAGC AATGATGGAG CAGGAAATGA CCCGGTTACA TCGCAGAGTG 
TCAGAGGTGG AGGCTGTGCT TAGTCAGAAG GAGGTGGAGC TGAAGGCCTC TGAGACTCAG 
AGATCCCTCC TGGAGCAGGA CCTTGCTACC TACATCACAG AATGCAGTAG CTTAAAGCGA 
AGTTTGGAGC AAGCGCGGAT GGAGGTGTCC CAGGAGGATG ACAAAGCTCT GCAGCTTCTC 
CACGACATCC GAGAGCAGAG CCGGAAGCTC CAGGAGATCA AGGAGCAGGA GTACCAGGCT 
CAGGTGGAGG AGATGAGGCT GATGATGAAT CAGCTGGAAG AAGACCTGGT GTCAGCCCGC 
AGACGCAGCG ATCTCTACGA GTCTGAGCTG AGGGAGTCTC GGCTTGCCGC CGAGGAATTC 
AAGCGGAAGG CAAACGAATG TCAGCACAAA CTGATGAAGG TAGTCAGCCA CCCTCCAAGA 
GGGGACTCCG GGGGCACGGC CCTGGATGAT CTTCACAAGA TGCAAGGCCA CGCTGGCTTA 
ACGAGTGCTA AGGACCAAGG GAAGCCTGAA GTGGGAGAAT ATTCCAAACT GGAGAAGATC 
AATGCTGAGC AGCAGCTGAA GATCCAGGAG CTCCAGGAGA AGCTGGAGAA GGCTGTAAAA 
GCCAGCACAG AGGCCACCGA GCTCCTGCAG AATATCCGCC AGGCAAAGGA GCGAGCAGAG 
CGGGAGCTAG AGAAGCTACA CAACCGGGAA GACTCCTCCG AGGGCATCAA AAAGAAGCTG 
GTGGAAGCCG AGGAGCTGGA GGAGAAACAT CGGGAGGCTC AGGTCTCAGC TCAACATCTA 
GAAGTACACT TGAAACAGAA AGAACAGCAC TACGAGGAAA AGATCAAGGT ATTGGACAAT 
CAGATAAAGA AGGACCTGGC CGACAAGGAG AGCCTGGAGA ACATGATGCA GAGACACGAG 
GAGGAGGCCC ACGAGAAGGG CAAGATCCTC AGCGAGCAGA AGGCGATGAT CAACGCGATG 
GATTCCAAGA TCCGATCCCT GGAGCAGAGG ATCGTGGAGC TGTCGGAAGC CAACAAGCTT 
GCGGCAAACA GCAGTCTCTT CACCCAGAGG AACATGAAGG CCCAGGAAGA GATGATCTCA 
GAACTCAGGC AGCAGAAATT TTACCTGGAG ACGCAGGCCG GAAAGCTGGA GGCCCAGAAC 
CGAAAGCTGG AAGAGCAACT GGAGAAGATC AGCCACCAAG ATCACAGTGA CAAGAGTCGG 
CTGCTGGAGC TGGAAACAAG GCTGAGGGAG GTCAGCCTGG AGCACGAGGA GCAGAAGCTG 
GAGCTGAAGC GGCAGCTCAC GGAGCTGCAG CTGTCCCTGC AGGAGCGCGA GTCCCAGCTG 
ACGGCCCTGC AGGCCGCCCG GGCAGCGCTG GAGAGCCAGC TCCGCCAGGC GAAGACAGAG 
CTGGAGGAGA CAACCGCGGA AGCGGAGGAG GAGATCCAGG CGCTCACGGC ACATCGAGAT 
GAGATCCAGC GCAAATTCGA TGCCCTTCGC AACAGCTGCA CGGTCATCAC CGACCTGGAG 
GAGCAGCTGA ACCAGCTCAC CGAGGACAAC GCCGAGCTCA ACAACCAAAA CTTCTACCTG 
TCCAAACAAC TCGATGAGGC TTCCGGGGCC AATGACGAGA TTGTGCAGCT GCGGAGTGAG 
GTGGACCATC TCCGCCGTGA GATCACGGAG CGGGAGATGC AGCTCACCAG CCAGAAGCAA 
GCACAACTCA GTGCTCCTGA TTTGCAGACG ATGGAGGCTC TGAAGACGAC ATGCACCATG 
CTGGAGGAGC AGGTCCTGGA CCTGGAGGCC CTGAACGACG AGCTGCTGGA GAAGGAGCGC 
CAGTGGGAGG CCTGGCGGAG CGTCCTCGGC GACGAGAAGT CCCAGTTCGA GTGTCGAGTT 
CGAGAGCTAC AGAGGATGCT GGACACCGAG AAGCAGAGCA GGGCTAGGGC CGATCAGCGG 
ATCACCGAGT CGCGCCAGGT GGTGGAGTTG GCGGTGAAGG AACACAAGGC CGAGATTCTT 
GCTCTGCAGC AGGCTCTCAA GGAGCAGAAG CTCAAAGCCG AGAGCCTGTC GGACAAGCTC 
AACGACCTGG AGAAGAAGCA CGCCATGCTG GAGATGAACG CCCGGAGCTT ACAGCAGAAA 
CTAGAGACAG AGCGGGAGCT CAAACAGAGG CTTCTGGAGG AGCAAGCCAA ATTGCAGCAG 
CAGATGGACC TGCAGAAGAA CCACATCTTC AGACTGACGC AAGGGCTGCA GGAGGCGCTG 
GACCGGGCGG ATCTGCTGAA GACAGAAAGG AGCGACCTGG AGTACCAGCT GGAAAACATT 
CAGGTTCTCT ACTCTCACGA GAAAGTGAAA ATGGAAGGCA CAATCTCTCA GCAAACCAAA 
CTCATTGATT TCCTGCAAGC CAAAATGGAC CAGCCTGCTA AAAAGAAAAA GGGTTTATTT 
AGTCGACGGA AAGAGGACCC TGCTTTGCCC ACACAGGTTC CTCTGCAGTA CAATGAGCTG 
AAGCTAGCCC TGGAAAAGGA GAAAGCCCGA TGCGCGGAGC TGGAGGAGGC CCTTCAGAAG 
ACCCGCATCG AACTCCGCTC TGCGCGGGAG GAAGCTGCCC ACCGCAAAGC CACGGACCAC 
CCGCACCCGT CTACGCCAGC CACTGCGAGG CAGCAGATCG CCATGTCTGC CATTGTGCGG 
TCGCCCGAGC ACCAGCCCAG TGCCATGAGC CTGCTTGCCC CACCATCCAG CCGCAGAAAG 
GAGTCGTCAA CTCCAGAGGA ATTCAGCCGG CGTCTGAAAG AGCGCATGCA CCACAACATC 
CCTCACCGGT TTAATGTGGG CCTGAACATG AGAGCCACCA AGTGCGCCGT GTGTCTGGAT 
ACTGTGCACT TTGGACGCCA GGCATCCAAA TGCCTCGAAT GTCAAGTGAT GTGTCACCCC 
AAATGCTCCA CCTGCTTGCC CGCTACCTGT GGCCTGCCAG CTGAATATGC CACACACTTC 
ACTGAGGCCT TCTGCCGGGA CAAAATGAAC TCCCCGGGGC TCCAGAGCAA GGAGCCTGGC 
AGCAGCTTGC ACCTGGAAGG GTGGATGAAA GTGCCCAGAA ATAACAAACG GGGACAGCAA 
GGCTGGGACA GGAAGTACAT TGTTCTGGAG GGGTCAAAAG TCCTCATCTA TGACAATGAA 
GCCAGAGAAG CTGGACAGAG GCCGGTGGAA GAATTTGAGC TGTGCCTTCC TGACGGGGAC 
GTATCTATTC ATGGTGCCGT TGGTGCTTCA GAACTTGCAA ATACCGCCAA AGCAGATGTC 
CCATACATCC TGAAGATGGA GTCTCACCCA CACACCACCT GCTGGCCTGG GAGAACCCTC 
TACTTGCTAG CACCCAGCTT CCCCGACAAG CAGCGCTGGG TCACCGCCTT AGAATCTGTC 
GTCGCAGGTG GGAGAGTTTC TAGGGAAAAG GCCGAAGCCG ATGCTGCACG TGACCATACT 
TCGAGTGAGC ATCAGCCAGT GTGGGTTGAG AAATTACTTG GAAACTCTCT GCTGAAACTG 
GAGGGCGATG ACCGGCTTGA CATGAACTGC ACCCTGCCCT TCAGTGACCA GGTAGTGCTG 
GTGGGCACGG AGGAAGGCCT CTACGCCCTG AATGTCTTGA AAAACTCCTT AACCCACATC 
CCAGGGATTG GCGCAGTCTT CCAAATTTAC ATCATCAAGG ACCTGGAGAA GCTGCTCATG 
ATAGCAGGGG AAGAGCGGGC TCTGTGCCTG GTGGACGTGA AGAAGGTGAA GCAGTCCCTG 
GCGCAGTCAC ACCTGCCTGC CCAGCCCGAC GTCTCCCCCA ACATATTCGA AGCCGTCAAA 
GGCTGCCACT TGTTCGCTGC TGGCAAGATC GAGAACAGCC TGTGCATCTG CGCCGCTATG 
CCAAGCAAAG TCGTCATCCT CCGCTACAAT GACAACCTCA GCAAGTACTG CATCCGCAAG 
GAGATCGAGA CCTCAGAGCC CTGCAGCTGT ATCCACTTCA CCAACTACAG CATCCTCATT 
GGGACCAACA AATTCTATGA GATCGACATG AAGCAGTACA CGCTTGACGA GTTCCTGGAC 
AAGAACGACC ATTCCTTGGC ACCTGCTGTG TTCGCCTCCT CGTCCAACAG CTTCCCTGTC 
TCCATTGTGC AGGCGAACAG CGCCGGGCAG CGAGAAGAAT ACCTGCTGTG CTTCCACGAA 
TTTGGGGTGT TCGTGGATTC TTACGGAAGA CGTAGCCGCA CAGATGATCT CAAGTGGAGT 
CGCTTACCTC TGGCCTTCGC CTACAGAGAA CCTTATCTGT TTGTGACTCA CTTCAACTCC 
CTGGAAGTCA TTGAGATCCA GGCCAGATCC TCACTGGGGA GCCCTGCCCG AGCATATCTG 
GAAATTCCAA ACCCTCGCTA CCTGGGCCCC GCGATTTCCT CCGGAGCGAT TTACCTGGCC 
TCCTCATACC AGGACAAGTT AAGGGTCATA TGCTGCAAAG GAAACCTCGT GAAGGAGTCA 
GGCACTGAGC AGCACCGGGT GCCCTCCACC TCCCGCAGCA GCCCCAACAA GCGAGGCCCA 
CCAACATACA ACGAGCACAT CACCAAACGC GTGGCCTCCA GCCCGGCGCC ACCGGAAGGC 
CCCAGCCACC CCCGAGAGCC AAGCACACCG CACCGCTACC GAGACAGAGA GGGTCGGACA 
GAGCTGCGCA GGGACAAGTC TCCAGGCCGC CCTCTGGAGC GGGAGAAGTC CCCAGGCCGA 
ATGCTCAGCA CTAGGAGAGA GCGGTCCCCA GGGAGACTGT TTGAAGACAG CAGCAGGGGC 
CGGCTGCCTG CAGGAGCAGT GAGGACCCCA CTGTCCCAGG TTAACAAGGT CTGGGACCAG 
TCTTCAGTAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cit ( XM_017320645.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017176134.1 Mus musculus citron (Cit), transcript variant X27, mRNA.

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CloneID	OMu81236
Clone ID Related Accession (Same CDS sequence)	XM_017320645.1 , XM_017320645.2
Accession Version	XM_017320645.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4845bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	citron Rho-interacting kinase isoform X23
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000071.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTCCTGG GCGAAGAAGC AATGATGGAG CAGGAAATGA CCCGGTTACA TCGCAGAGTG 
TCAGAGGTGG AGGCTGTGCT TAGTCAGAAG GAGGTGGAGC TGAAGGCCTC TGAGACTCAG 
AGATCCCTCC TGGAGCAGGA CCTTGCTACC TACATCACAG AATGCAGTAG CTTAAAGCGA 
AGTTTGGAGC AAGCGCGGAT GGAGGTGTCC CAGGAGGATG ACAAAGCTCT GCAGCTTCTC 
CACGACATCC GAGAGCAGAG CCGGAAGCTC CAGGAGATCA AGGAGCAGGA GTACCAGGCT 
CAGGTGGAGG AGATGAGGCT GATGATGAAT CAGCTGGAAG AAGACCTGGT GTCAGCCCGC 
AGACGCAGCG ATCTCTACGA GTCTGAGCTG AGGGAGTCTC GGCTTGCCGC CGAGGAATTC 
AAGCGGAAGG CAAACGAATG TCAGCACAAA CTGATGAAGG TAGTCAGCCA CCCTCCAAGA 
GGGGACTCCG GGGGCACGGC CCTGGATGAT CTTCACAAGA TGCAAGGCCA CGCTGGCTTA 
ACGAGTGCTA AGGACCAAGG GAAGCCTGAA GTGGGAGAAT ATTCCAAACT GGAGAAGATC 
AATGCTGAGC AGCAGCTGAA GATCCAGGAG CTCCAGGAGA AGCTGGAGAA GGCTGTAAAA 
GCCAGCACAG AGGCCACCGA GCTCCTGCAG AATATCCGCC AGGCAAAGGA GCGAGCAGAG 
CGGGAGCTAG AGAAGCTACA CAACCGGGAA GACTCCTCCG AGGGCATCAA AAAGAAGCTG 
GTGGAAGCCG AGGAGCTGGA GGAGAAACAT CGGGAGGCTC AGGTCTCAGC TCAACATCTA 
GAAGTACACT TGAAACAGAA AGAACAGCAC TACGAGGAAA AGATCAAGGT ATTGGACAAT 
CAGATAAAGA AGGACCTGGC CGACAAGGAG AGCCTGGAGA ACATGATGCA GAGACACGAG 
GAGGAGGCCC ACGAGAAGGG CAAGATCCTC AGCGAGCAGA AGGCGATGAT CAACGCGATG 
GATTCCAAGA TCCGATCCCT GGAGCAGAGG ATCGTGGAGC TGTCGGAAGC CAACAAGCTT 
GCGGCAAACA GCAGTCTCTT CACCCAGAGG AACATGAAGG CCCAGGAAGA GATGATCTCA 
GAACTCAGGC AGCAGAAATT TTACCTGGAG ACGCAGGCCG GAAAGCTGGA GGCCCAGAAC 
CGAAAGCTGG AAGAGCAACT GGAGAAGATC AGCCACCAAG ATCACAGTGA CAAGAGTCGG 
CTGCTGGAGC TGGAAACAAG GCTGAGGGAG GTCAGCCTGG AGCACGAGGA GCAGAAGCTG 
GAGCTGAAGC GGCAGCTCAC GGAGCTGCAG CTGTCCCTGC AGGAGCGCGA GTCCCAGCTG 
ACGGCCCTGC AGGCCGCCCG GGCAGCGCTG GAGAGCCAGC TCCGCCAGGC GAAGACAGAG 
CTGGAGGAGA CAACCGCGGA AGCGGAGGAG GAGATCCAGG CGCTCACGGC ACATCGAGAT 
GAGATCCAGC GCAAATTCGA TGCCCTTCGC AACAGCTGCA CGGTCATCAC CGACCTGGAG 
GAGCAGCTGA ACCAGCTCAC CGAGGACAAC GCCGAGCTCA ACAACCAAAA CTTCTACCTG 
TCCAAACAAC TCGATGAGGC TTCCGGGGCC AATGACGAGA TTGTGCAGCT GCGGAGTGAG 
GTGGACCATC TCCGCCGTGA GATCACGGAG CGGGAGATGC AGCTCACCAG CCAGAAGCAA 
ACGATGGAGG CTCTGAAGAC GACATGCACC ATGCTGGAGG AGCAGGTCCT GGACCTGGAG 
GCCCTGAACG ACGAGCTGCT GGAGAAGGAG CGCCAGTGGG AGGCCTGGCG GAGCGTCCTC 
GGCGACGAGA AGTCCCAGTT CGAGTGTCGA GTTCGAGAGC TACAGAGGAT GCTGGACACC 
GAGAAGCAGA GCAGGGCTAG GGCCGATCAG CGGATCACCG AGTCGCGCCA GGTGGTGGAG 
TTGGCGGTGA AGGAACACAA GGCCGAGATT CTTGCTCTGC AGCAGGCTCT CAAGGAGCAG 
AAGCTCAAAG CCGAGAGCCT GTCGGACAAG CTCAACGACC TGGAGAAGAA GCACGCCATG 
CTGGAGATGA ACGCCCGGAG CTTACAGCAG AAACTAGAGA CAGAGCGGGA GCTCAAACAG 
AGGCTTCTGG AGGAGCAAGC CAAATTGCAG CAGCAGATGG ACCTGCAGAA GAACCACATC 
TTCAGACTGA CGCAAGGGCT GCAGGAGGCG CTGGACCGGG CGGATCTGCT GAAGACAGAA 
AGGAGCGACC TGGAGTACCA GCTGGAAAAC ATTCAGGTTC TCTACTCTCA CGAGAAAGTG 
AAAATGGAAG GCACAATCTC TCAGCAAACC AAACTCATTG ATTTCCTGCA AGCCAAAATG 
GACCAGCCTG CTAAAAAGAA AAAGGGTTTA TTTAGTCGAC GGAAAGAGGA CCCTGCTTTG 
CCCACACAGG TTCCTCTGCA GTACAATGAG CTGAAGCTAG CCCTGGAAAA GGAGAAAGCC 
CGATGCGCGG AGCTGGAGGA GGCCCTTCAG AAGACCCGCA TCGAACTCCG CTCTGCGCGG 
GAGGAAGCTG CCCACCGCAA AGCCACGGAC CACCCGCACC CGTCTACGCC AGCCACTGCG 
AGGCAGCAGA TCGCCATGTC TGCCATTGTG CGGTCGCCCG AGCACCAGCC CAGTGCCATG 
AGCCTGCTTG CCCCACCATC CAGCCGCAGA AAGGAGTCGT CAACTCCAGA GGAATTCAGC 
CGGCGTCTGA AAGAGCGCAT GCACCACAAC ATCCCTCACC GGTTTAATGT GGGCCTGAAC 
ATGAGAGCCA CCAAGTGCGC CGTGTGTCTG GATACTGTGC ACTTTGGACG CCAGGCATCC 
AAATGCCTCG AATGTCAAGT GATGTGTCAC CCCAAATGCT CCACCTGCTT GCCCGCTACC 
TGTGGCCTGC CAGCTGAATA TGCCACACAC TTCACTGAGG CCTTCTGCCG GGACAAAATG 
AACTCCCCGG GGCTCCAGAG CAAGGAGCCT GGCAGCAGCT TGCACCTGGA AGGGTGGATG 
AAAGTGCCCA GAAATAACAA ACGGGGACAG CAAGGCTGGG ACAGGAAGTA CATTGTTCTG 
GAGGGGTCAA AAGTCCTCAT CTATGACAAT GAAGCCAGAG AAGCTGGACA GAGGCCGGTG 
GAAGAATTTG AGCTGTGCCT TCCTGACGGG GACGTATCTA TTCATGGTGC CGTTGGTGCT 
TCAGAACTTG CAAATACCGC CAAAGCAGAT GTCCCATACA TCCTGAAGAT GGAGTCTCAC 
CCACACACCA CCTGCTGGCC TGGGAGAACC CTCTACTTGC TAGCACCCAG CTTCCCCGAC 
AAGCAGCGCT GGGTCACCGC CTTAGAATCT GTCGTCGCAG GTGGGAGAGT TTCTAGGGAA 
AAGGCCGAAG CCGATGCTGC ACGTGACCAT ACTTCGAGTG AGCATCAGCC AGTGTGGGTT 
GAGAAATTAC TTGGAAACTC TCTGCTGAAA CTGGAGGGCG ATGACCGGCT TGACATGAAC 
TGCACCCTGC CCTTCAGTGA CCAGGTAGTG CTGGTGGGCA CGGAGGAAGG CCTCTACGCC 
CTGAATGTCT TGAAAAACTC CTTAACCCAC ATCCCAGGGA TTGGCGCAGT CTTCCAAATT 
TACATCATCA AGGACCTGGA GAAGCTGCTC ATGATAGCAG GGGAAGAGCG GGCTCTGTGC 
CTGGTGGACG TGAAGAAGGT GAAGCAGTCC CTGGCGCAGT CACACCTGCC TGCCCAGCCC 
GACGTCTCCC CCAACATATT CGAAGCCGTC AAAGGCTGCC ACTTGTTCGC TGCTGGCAAG 
ATCGAGAACA GCCTGTGCAT CTGCGCCGCT ATGCCAAGCA AAGTCGTCAT CCTCCGCTAC 
AATGACAACC TCAGCAAGTA CTGCATCCGC AAGGAGATCG AGACCTCAGA GCCCTGCAGC 
TGTATCCACT TCACCAACTA CAGCATCCTC ATTGGGACCA ACAAATTCTA TGAGATCGAC 
ATGAAGCAGT ACACGCTTGA CGAGTTCCTG GACAAGAACG ACCATTCCTT GGCACCTGCT 
GTGTTCGCCT CCTCGTCCAA CAGCTTCCCT GTCTCCATTG TGCAGGCGAA CAGCGCCGGG 
CAGCGAGAAG AATACCTGCT GTGCTTCCAC GAATTTGGGG TGTTCGTGGA TTCTTACGGA 
AGACGTAGCC GCACAGATGA TCTCAAGTGG AGTCGCTTAC CTCTGGCCTT CGCCTACAGA 
GAACCTTATC TGTTTGTGAC TCACTTCAAC TCCCTGGAAG TCATTGAGAT CCAGGCCAGA 
TCCTCACTGG GGAGCCCTGC CCGAGCATAT CTGGAAATTC CAAACCCTCG CTACCTGGGC 
CCCGCGATTT CCTCCGGAGC GATTTACCTG GCCTCCTCAT ACCAGGACAA GTTAAGGGTC 
ATATGCTGCA AAGGAAACCT CGTGAAGGAG TCAGGCACTG AGCAGCACCG GGTGCCCTCC 
ACCTCCCGCA GCAGCCCCAA CAAGCGAGGC CCACCAACAT ACAACGAGCA CATCACCAAA 
CGCGTGGCCT CCAGCCCGGC GCCACCGGAA GGCCCCAGCC ACCCCCGAGA GCCAAGCACA 
CCGCACCGCT ACCGAGACAG AGAGGGTCGG ACAGAGCTGC GCAGGGACAA GTCTCCAGGC 
CGCCCTCTGG AGCGGGAGAA GTCCCCAGGC CGAATGCTCA GCACTAGGAG AGAGCGGTCC 
CCAGGGAGAC TGTTTGAAGA CAGCAGCAGG GGCCGGCTGC CTGCAGGAGC AGTGAGGACC 
CCACTGTCCC AGGTTAACAA GGTCTGGGAC CAGTCTTCAG TATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017176134.1
CDS	1419..6263
Translation	MLLGEEAMMEQEMTRLHRRVSEVEAVLSQKEVELKASETQRSLLEQDLATYITECSSLKRSLEQARMEVSQEDDKALQLLHDIREQSRKLQEIKEQEYQAQVEEMRLMMNQLEEDLVSARRRSDLYESELRESRLAAEEFKRKANECQHKLMKVVSHPPRGDSGGTALDDLHKMQGHAGLTSAKDQGKPEVGEYSKLEKINAEQQLKIQELQEKLEKAVKASTEATELLQNIRQAKERAERELEKLHNREDSSEGIKKKLVEAEELEEKHREAQVSAQHLEVHLKQKEQHYEEKIKVLDNQIKKDLADKESLENMMQRHEEEAHEKGKILSEQKAMINAMDSKIRSLEQRIVELSEANKLAANSSLFTQRNMKAQEEMISELRQQKFYLETQAGKLEAQNRKLEEQLEKISHQDHSDKSRLLELETRLREVSLEHEEQKLELKRQLTELQLSLQERESQLTALQAARAALESQLRQAKTELEETTAEAEEEIQALTAHRDEIQRKFDALRNSCTVITDLEEQLNQLTEDNAELNNQNFYLSKQLDEASGANDEIVQLRSEVDHLRREITEREMQLTSQKQTMEALKTTCTMLEEQVLDLEALNDELLEKERQWEAWRSVLGDEKSQFECRVRELQRMLDTEKQSRARADQRITESRQVVELAVKEHKAEILALQQALKEQKLKAESLSDKLNDLEKKHAMLEMNARSLQQKLETERELKQRLLEEQAKLQQQMDLQKNHIFRLTQGLQEALDRADLLKTERSDLEYQLENIQVLYSHEKVKMEGTISQQTKLIDFLQAKMDQPAKKKKGLFSRRKEDPALPTQVPLQYNELKLALEKEKARCAELEEALQKTRIELRSAREEAAHRKATDHPHPSTPATARQQIAMSAIVRSPEHQPSAMSLLAPPSSRRKESSTPEEFSRRLKERMHHNIPHRFNVGLNMRATKCAVCLDTVHFGRQASKCLECQVMCHPKCSTCLPATCGLPAEYATHFTEAFCRDKMNSPGLQSKEPGSSLHLEGWMKVPRNNKRGQQGWDRKYIVLEGSKVLIYDNEAREAGQRPVEEFELCLPDGDVSIHGAVGASELANTAKADVPYILKMESHPHTTCWPGRTLYLLAPSFPDKQRWVTALESVVAGGRVSREKAEADAARDHTSSEHQPVWVEKLLGNSLLKLEGDDRLDMNCTLPFSDQVVLVGTEEGLYALNVLKNSLTHIPGIGAVFQIYIIKDLEKLLMIAGEERALCLVDVKKVKQSLAQSHLPAQPDVSPNIFEAVKGCHLFAAGKIENSLCICAAMPSKVVILRYNDNLSKYCIRKEIETSEPCSCIHFTNYSILIGTNKFYEIDMKQYTLDEFLDKNDHSLAPAVFASSSNSFPVSIVQANSAGQREEYLLCFHEFGVFVDSYGRRSRTDDLKWSRLPLAFAYREPYLFVTHFNSLEVIEIQARSSLGSPARAYLEIPNPRYLGPAISSGAIYLASSYQDKLRVICCKGNLVKESGTEQHRVPSTSRSSPNKRGPPTYNEHITKRVASSPAPPEGPSHPREPSTPHRYRDREGRTELRRDKSPGRPLEREKSPGRMLSTRRERSPGRLFEDSSRGRLPAGAVRTPLSQVNKVWDQSSV

Target ORF information:

RefSeq Version	XM_017320645.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus citron (Cit), transcript variant X27, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017320645.1

ORF Insert Sequence:

ATGCTCCTGG GCGAAGAAGC AATGATGGAG CAGGAAATGA CCCGGTTACA TCGCAGAGTG 
TCAGAGGTGG AGGCTGTGCT TAGTCAGAAG GAGGTGGAGC TGAAGGCCTC TGAGACTCAG 
AGATCCCTCC TGGAGCAGGA CCTTGCTACC TACATCACAG AATGCAGTAG CTTAAAGCGA 
AGTTTGGAGC AAGCGCGGAT GGAGGTGTCC CAGGAGGATG ACAAAGCTCT GCAGCTTCTC 
CACGACATCC GAGAGCAGAG CCGGAAGCTC CAGGAGATCA AGGAGCAGGA GTACCAGGCT 
CAGGTGGAGG AGATGAGGCT GATGATGAAT CAGCTGGAAG AAGACCTGGT GTCAGCCCGC 
AGACGCAGCG ATCTCTACGA GTCTGAGCTG AGGGAGTCTC GGCTTGCCGC CGAGGAATTC 
AAGCGGAAGG CAAACGAATG TCAGCACAAA CTGATGAAGG TAGTCAGCCA CCCTCCAAGA 
GGGGACTCCG GGGGCACGGC CCTGGATGAT CTTCACAAGA TGCAAGGCCA CGCTGGCTTA 
ACGAGTGCTA AGGACCAAGG GAAGCCTGAA GTGGGAGAAT ATTCCAAACT GGAGAAGATC 
AATGCTGAGC AGCAGCTGAA GATCCAGGAG CTCCAGGAGA AGCTGGAGAA GGCTGTAAAA 
GCCAGCACAG AGGCCACCGA GCTCCTGCAG AATATCCGCC AGGCAAAGGA GCGAGCAGAG 
CGGGAGCTAG AGAAGCTACA CAACCGGGAA GACTCCTCCG AGGGCATCAA AAAGAAGCTG 
GTGGAAGCCG AGGAGCTGGA GGAGAAACAT CGGGAGGCTC AGGTCTCAGC TCAACATCTA 
GAAGTACACT TGAAACAGAA AGAACAGCAC TACGAGGAAA AGATCAAGGT ATTGGACAAT 
CAGATAAAGA AGGACCTGGC CGACAAGGAG AGCCTGGAGA ACATGATGCA GAGACACGAG 
GAGGAGGCCC ACGAGAAGGG CAAGATCCTC AGCGAGCAGA AGGCGATGAT CAACGCGATG 
GATTCCAAGA TCCGATCCCT GGAGCAGAGG ATCGTGGAGC TGTCGGAAGC CAACAAGCTT 
GCGGCAAACA GCAGTCTCTT CACCCAGAGG AACATGAAGG CCCAGGAAGA GATGATCTCA 
GAACTCAGGC AGCAGAAATT TTACCTGGAG ACGCAGGCCG GAAAGCTGGA GGCCCAGAAC 
CGAAAGCTGG AAGAGCAACT GGAGAAGATC AGCCACCAAG ATCACAGTGA CAAGAGTCGG 
CTGCTGGAGC TGGAAACAAG GCTGAGGGAG GTCAGCCTGG AGCACGAGGA GCAGAAGCTG 
GAGCTGAAGC GGCAGCTCAC GGAGCTGCAG CTGTCCCTGC AGGAGCGCGA GTCCCAGCTG 
ACGGCCCTGC AGGCCGCCCG GGCAGCGCTG GAGAGCCAGC TCCGCCAGGC GAAGACAGAG 
CTGGAGGAGA CAACCGCGGA AGCGGAGGAG GAGATCCAGG CGCTCACGGC ACATCGAGAT 
GAGATCCAGC GCAAATTCGA TGCCCTTCGC AACAGCTGCA CGGTCATCAC CGACCTGGAG 
GAGCAGCTGA ACCAGCTCAC CGAGGACAAC GCCGAGCTCA ACAACCAAAA CTTCTACCTG 
TCCAAACAAC TCGATGAGGC TTCCGGGGCC AATGACGAGA TTGTGCAGCT GCGGAGTGAG 
GTGGACCATC TCCGCCGTGA GATCACGGAG CGGGAGATGC AGCTCACCAG CCAGAAGCAA 
ACGATGGAGG CTCTGAAGAC GACATGCACC ATGCTGGAGG AGCAGGTCCT GGACCTGGAG 
GCCCTGAACG ACGAGCTGCT GGAGAAGGAG CGCCAGTGGG AGGCCTGGCG GAGCGTCCTC 
GGCGACGAGA AGTCCCAGTT CGAGTGTCGA GTTCGAGAGC TACAGAGGAT GCTGGACACC 
GAGAAGCAGA GCAGGGCTAG GGCCGATCAG CGGATCACCG AGTCGCGCCA GGTGGTGGAG 
TTGGCGGTGA AGGAACACAA GGCCGAGATT CTTGCTCTGC AGCAGGCTCT CAAGGAGCAG 
AAGCTCAAAG CCGAGAGCCT GTCGGACAAG CTCAACGACC TGGAGAAGAA GCACGCCATG 
CTGGAGATGA ACGCCCGGAG CTTACAGCAG AAACTAGAGA CAGAGCGGGA GCTCAAACAG 
AGGCTTCTGG AGGAGCAAGC CAAATTGCAG CAGCAGATGG ACCTGCAGAA GAACCACATC 
TTCAGACTGA CGCAAGGGCT GCAGGAGGCG CTGGACCGGG CGGATCTGCT GAAGACAGAA 
AGGAGCGACC TGGAGTACCA GCTGGAAAAC ATTCAGGTTC TCTACTCTCA CGAGAAAGTG 
AAAATGGAAG GCACAATCTC TCAGCAAACC AAACTCATTG ATTTCCTGCA AGCCAAAATG 
GACCAGCCTG CTAAAAAGAA AAAGGGTTTA TTTAGTCGAC GGAAAGAGGA CCCTGCTTTG 
CCCACACAGG TTCCTCTGCA GTACAATGAG CTGAAGCTAG CCCTGGAAAA GGAGAAAGCC 
CGATGCGCGG AGCTGGAGGA GGCCCTTCAG AAGACCCGCA TCGAACTCCG CTCTGCGCGG 
GAGGAAGCTG CCCACCGCAA AGCCACGGAC CACCCGCACC CGTCTACGCC AGCCACTGCG 
AGGCAGCAGA TCGCCATGTC TGCCATTGTG CGGTCGCCCG AGCACCAGCC CAGTGCCATG 
AGCCTGCTTG CCCCACCATC CAGCCGCAGA AAGGAGTCGT CAACTCCAGA GGAATTCAGC 
CGGCGTCTGA AAGAGCGCAT GCACCACAAC ATCCCTCACC GGTTTAATGT GGGCCTGAAC 
ATGAGAGCCA CCAAGTGCGC CGTGTGTCTG GATACTGTGC ACTTTGGACG CCAGGCATCC 
AAATGCCTCG AATGTCAAGT GATGTGTCAC CCCAAATGCT CCACCTGCTT GCCCGCTACC 
TGTGGCCTGC CAGCTGAATA TGCCACACAC TTCACTGAGG CCTTCTGCCG GGACAAAATG 
AACTCCCCGG GGCTCCAGAG CAAGGAGCCT GGCAGCAGCT TGCACCTGGA AGGGTGGATG 
AAAGTGCCCA GAAATAACAA ACGGGGACAG CAAGGCTGGG ACAGGAAGTA CATTGTTCTG 
GAGGGGTCAA AAGTCCTCAT CTATGACAAT GAAGCCAGAG AAGCTGGACA GAGGCCGGTG 
GAAGAATTTG AGCTGTGCCT TCCTGACGGG GACGTATCTA TTCATGGTGC CGTTGGTGCT 
TCAGAACTTG CAAATACCGC CAAAGCAGAT GTCCCATACA TCCTGAAGAT GGAGTCTCAC 
CCACACACCA CCTGCTGGCC TGGGAGAACC CTCTACTTGC TAGCACCCAG CTTCCCCGAC 
AAGCAGCGCT GGGTCACCGC CTTAGAATCT GTCGTCGCAG GTGGGAGAGT TTCTAGGGAA 
AAGGCCGAAG CCGATGCTGC ACGTGACCAT ACTTCGAGTG AGCATCAGCC AGTGTGGGTT 
GAGAAATTAC TTGGAAACTC TCTGCTGAAA CTGGAGGGCG ATGACCGGCT TGACATGAAC 
TGCACCCTGC CCTTCAGTGA CCAGGTAGTG CTGGTGGGCA CGGAGGAAGG CCTCTACGCC 
CTGAATGTCT TGAAAAACTC CTTAACCCAC ATCCCAGGGA TTGGCGCAGT CTTCCAAATT 
TACATCATCA AGGACCTGGA GAAGCTGCTC ATGATAGCAG GGGAAGAGCG GGCTCTGTGC 
CTGGTGGACG TGAAGAAGGT GAAGCAGTCC CTGGCGCAGT CACACCTGCC TGCCCAGCCC 
GACGTCTCCC CCAACATATT CGAAGCCGTC AAAGGCTGCC ACTTGTTCGC TGCTGGCAAG 
ATCGAGAACA GCCTGTGCAT CTGCGCCGCT ATGCCAAGCA AAGTCGTCAT CCTCCGCTAC 
AATGACAACC TCAGCAAGTA CTGCATCCGC AAGGAGATCG AGACCTCAGA GCCCTGCAGC 
TGTATCCACT TCACCAACTA CAGCATCCTC ATTGGGACCA ACAAATTCTA TGAGATCGAC 
ATGAAGCAGT ACACGCTTGA CGAGTTCCTG GACAAGAACG ACCATTCCTT GGCACCTGCT 
GTGTTCGCCT CCTCGTCCAA CAGCTTCCCT GTCTCCATTG TGCAGGCGAA CAGCGCCGGG 
CAGCGAGAAG AATACCTGCT GTGCTTCCAC GAATTTGGGG TGTTCGTGGA TTCTTACGGA 
AGACGTAGCC GCACAGATGA TCTCAAGTGG AGTCGCTTAC CTCTGGCCTT CGCCTACAGA 
GAACCTTATC TGTTTGTGAC TCACTTCAAC TCCCTGGAAG TCATTGAGAT CCAGGCCAGA 
TCCTCACTGG GGAGCCCTGC CCGAGCATAT CTGGAAATTC CAAACCCTCG CTACCTGGGC 
CCCGCGATTT CCTCCGGAGC GATTTACCTG GCCTCCTCAT ACCAGGACAA GTTAAGGGTC 
ATATGCTGCA AAGGAAACCT CGTGAAGGAG TCAGGCACTG AGCAGCACCG GGTGCCCTCC 
ACCTCCCGCA GCAGCCCCAA CAAGCGAGGC CCACCAACAT ACAACGAGCA CATCACCAAA 
CGCGTGGCCT CCAGCCCGGC GCCACCGGAA GGCCCCAGCC ACCCCCGAGA GCCAAGCACA 
CCGCACCGCT ACCGAGACAG AGAGGGTCGG ACAGAGCTGC GCAGGGACAA GTCTCCAGGC 
CGCCCTCTGG AGCGGGAGAA GTCCCCAGGC CGAATGCTCA GCACTAGGAG AGAGCGGTCC 
CCAGGGAGAC TGTTTGAAGA CAGCAGCAGG GGCCGGCTGC CTGCAGGAGC AGTGAGGACC 
CCACTGTCCC AGGTTAACAA GGTCTGGGAC CAGTCTTCAG TATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cit ( XM_017320645.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017176134.1 Mus musculus citron (Cit), transcript variant X29, mRNA.

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CloneID	OMu81236
Clone ID Related Accession (Same CDS sequence)	XM_017320645.1 , XM_017320645.2
Accession Version	XM_017320645.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4845bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	citron Rho-interacting kinase isoform X24
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000071.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_017320645.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTCCTGG GCGAAGAAGC AATGATGGAG CAGGAAATGA CCCGGTTACA TCGCAGAGTG 
TCAGAGGTGG AGGCTGTGCT TAGTCAGAAG GAGGTGGAGC TGAAGGCCTC TGAGACTCAG 
AGATCCCTCC TGGAGCAGGA CCTTGCTACC TACATCACAG AATGCAGTAG CTTAAAGCGA 
AGTTTGGAGC AAGCGCGGAT GGAGGTGTCC CAGGAGGATG ACAAAGCTCT GCAGCTTCTC 
CACGACATCC GAGAGCAGAG CCGGAAGCTC CAGGAGATCA AGGAGCAGGA GTACCAGGCT 
CAGGTGGAGG AGATGAGGCT GATGATGAAT CAGCTGGAAG AAGACCTGGT GTCAGCCCGC 
AGACGCAGCG ATCTCTACGA GTCTGAGCTG AGGGAGTCTC GGCTTGCCGC CGAGGAATTC 
AAGCGGAAGG CAAACGAATG TCAGCACAAA CTGATGAAGG TAGTCAGCCA CCCTCCAAGA 
GGGGACTCCG GGGGCACGGC CCTGGATGAT CTTCACAAGA TGCAAGGCCA CGCTGGCTTA 
ACGAGTGCTA AGGACCAAGG GAAGCCTGAA GTGGGAGAAT ATTCCAAACT GGAGAAGATC 
AATGCTGAGC AGCAGCTGAA GATCCAGGAG CTCCAGGAGA AGCTGGAGAA GGCTGTAAAA 
GCCAGCACAG AGGCCACCGA GCTCCTGCAG AATATCCGCC AGGCAAAGGA GCGAGCAGAG 
CGGGAGCTAG AGAAGCTACA CAACCGGGAA GACTCCTCCG AGGGCATCAA AAAGAAGCTG 
GTGGAAGCCG AGGAGCTGGA GGAGAAACAT CGGGAGGCTC AGGTCTCAGC TCAACATCTA 
GAAGTACACT TGAAACAGAA AGAACAGCAC TACGAGGAAA AGATCAAGGT ATTGGACAAT 
CAGATAAAGA AGGACCTGGC CGACAAGGAG AGCCTGGAGA ACATGATGCA GAGACACGAG 
GAGGAGGCCC ACGAGAAGGG CAAGATCCTC AGCGAGCAGA AGGCGATGAT CAACGCGATG 
GATTCCAAGA TCCGATCCCT GGAGCAGAGG ATCGTGGAGC TGTCGGAAGC CAACAAGCTT 
GCGGCAAACA GCAGTCTCTT CACCCAGAGG AACATGAAGG CCCAGGAAGA GATGATCTCA 
GAACTCAGGC AGCAGAAATT TTACCTGGAG ACGCAGGCCG GAAAGCTGGA GGCCCAGAAC 
CGAAAGCTGG AAGAGCAACT GGAGAAGATC AGCCACCAAG ATCACAGTGA CAAGAGTCGG 
CTGCTGGAGC TGGAAACAAG GCTGAGGGAG GTCAGCCTGG AGCACGAGGA GCAGAAGCTG 
GAGCTGAAGC GGCAGCTCAC GGAGCTGCAG CTGTCCCTGC AGGAGCGCGA GTCCCAGCTG 
ACGGCCCTGC AGGCCGCCCG GGCAGCGCTG GAGAGCCAGC TCCGCCAGGC GAAGACAGAG 
CTGGAGGAGA CAACCGCGGA AGCGGAGGAG GAGATCCAGG CGCTCACGGC ACATCGAGAT 
GAGATCCAGC GCAAATTCGA TGCCCTTCGC AACAGCTGCA CGGTCATCAC CGACCTGGAG 
GAGCAGCTGA ACCAGCTCAC CGAGGACAAC GCCGAGCTCA ACAACCAAAA CTTCTACCTG 
TCCAAACAAC TCGATGAGGC TTCCGGGGCC AATGACGAGA TTGTGCAGCT GCGGAGTGAG 
GTGGACCATC TCCGCCGTGA GATCACGGAG CGGGAGATGC AGCTCACCAG CCAGAAGCAA 
ACGATGGAGG CTCTGAAGAC GACATGCACC ATGCTGGAGG AGCAGGTCCT GGACCTGGAG 
GCCCTGAACG ACGAGCTGCT GGAGAAGGAG CGCCAGTGGG AGGCCTGGCG GAGCGTCCTC 
GGCGACGAGA AGTCCCAGTT CGAGTGTCGA GTTCGAGAGC TACAGAGGAT GCTGGACACC 
GAGAAGCAGA GCAGGGCTAG GGCCGATCAG CGGATCACCG AGTCGCGCCA GGTGGTGGAG 
TTGGCGGTGA AGGAACACAA GGCCGAGATT CTTGCTCTGC AGCAGGCTCT CAAGGAGCAG 
AAGCTCAAAG CCGAGAGCCT GTCGGACAAG CTCAACGACC TGGAGAAGAA GCACGCCATG 
CTGGAGATGA ACGCCCGGAG CTTACAGCAG AAACTAGAGA CAGAGCGGGA GCTCAAACAG 
AGGCTTCTGG AGGAGCAAGC CAAATTGCAG CAGCAGATGG ACCTGCAGAA GAACCACATC 
TTCAGACTGA CGCAAGGGCT GCAGGAGGCG CTGGACCGGG CGGATCTGCT GAAGACAGAA 
AGGAGCGACC TGGAGTACCA GCTGGAAAAC ATTCAGGTTC TCTACTCTCA CGAGAAAGTG 
AAAATGGAAG GCACAATCTC TCAGCAAACC AAACTCATTG ATTTCCTGCA AGCCAAAATG 
GACCAGCCTG CTAAAAAGAA AAAGGGTTTA TTTAGTCGAC GGAAAGAGGA CCCTGCTTTG 
CCCACACAGG TTCCTCTGCA GTACAATGAG CTGAAGCTAG CCCTGGAAAA GGAGAAAGCC 
CGATGCGCGG AGCTGGAGGA GGCCCTTCAG AAGACCCGCA TCGAACTCCG CTCTGCGCGG 
GAGGAAGCTG CCCACCGCAA AGCCACGGAC CACCCGCACC CGTCTACGCC AGCCACTGCG 
AGGCAGCAGA TCGCCATGTC TGCCATTGTG CGGTCGCCCG AGCACCAGCC CAGTGCCATG 
AGCCTGCTTG CCCCACCATC CAGCCGCAGA AAGGAGTCGT CAACTCCAGA GGAATTCAGC 
CGGCGTCTGA AAGAGCGCAT GCACCACAAC ATCCCTCACC GGTTTAATGT GGGCCTGAAC 
ATGAGAGCCA CCAAGTGCGC CGTGTGTCTG GATACTGTGC ACTTTGGACG CCAGGCATCC 
AAATGCCTCG AATGTCAAGT GATGTGTCAC CCCAAATGCT CCACCTGCTT GCCCGCTACC 
TGTGGCCTGC CAGCTGAATA TGCCACACAC TTCACTGAGG CCTTCTGCCG GGACAAAATG 
AACTCCCCGG GGCTCCAGAG CAAGGAGCCT GGCAGCAGCT TGCACCTGGA AGGGTGGATG 
AAAGTGCCCA GAAATAACAA ACGGGGACAG CAAGGCTGGG ACAGGAAGTA CATTGTTCTG 
GAGGGGTCAA AAGTCCTCAT CTATGACAAT GAAGCCAGAG AAGCTGGACA GAGGCCGGTG 
GAAGAATTTG AGCTGTGCCT TCCTGACGGG GACGTATCTA TTCATGGTGC CGTTGGTGCT 
TCAGAACTTG CAAATACCGC CAAAGCAGAT GTCCCATACA TCCTGAAGAT GGAGTCTCAC 
CCACACACCA CCTGCTGGCC TGGGAGAACC CTCTACTTGC TAGCACCCAG CTTCCCCGAC 
AAGCAGCGCT GGGTCACCGC CTTAGAATCT GTCGTCGCAG GTGGGAGAGT TTCTAGGGAA 
AAGGCCGAAG CCGATGCTGC ACGTGACCAT ACTTCGAGTG AGCATCAGCC AGTGTGGGTT 
GAGAAATTAC TTGGAAACTC TCTGCTGAAA CTGGAGGGCG ATGACCGGCT TGACATGAAC 
TGCACCCTGC CCTTCAGTGA CCAGGTAGTG CTGGTGGGCA CGGAGGAAGG CCTCTACGCC 
CTGAATGTCT TGAAAAACTC CTTAACCCAC ATCCCAGGGA TTGGCGCAGT CTTCCAAATT 
TACATCATCA AGGACCTGGA GAAGCTGCTC ATGATAGCAG GGGAAGAGCG GGCTCTGTGC 
CTGGTGGACG TGAAGAAGGT GAAGCAGTCC CTGGCGCAGT CACACCTGCC TGCCCAGCCC 
GACGTCTCCC CCAACATATT CGAAGCCGTC AAAGGCTGCC ACTTGTTCGC TGCTGGCAAG 
ATCGAGAACA GCCTGTGCAT CTGCGCCGCT ATGCCAAGCA AAGTCGTCAT CCTCCGCTAC 
AATGACAACC TCAGCAAGTA CTGCATCCGC AAGGAGATCG AGACCTCAGA GCCCTGCAGC 
TGTATCCACT TCACCAACTA CAGCATCCTC ATTGGGACCA ACAAATTCTA TGAGATCGAC 
ATGAAGCAGT ACACGCTTGA CGAGTTCCTG GACAAGAACG ACCATTCCTT GGCACCTGCT 
GTGTTCGCCT CCTCGTCCAA CAGCTTCCCT GTCTCCATTG TGCAGGCGAA CAGCGCCGGG 
CAGCGAGAAG AATACCTGCT GTGCTTCCAC GAATTTGGGG TGTTCGTGGA TTCTTACGGA 
AGACGTAGCC GCACAGATGA TCTCAAGTGG AGTCGCTTAC CTCTGGCCTT CGCCTACAGA 
GAACCTTATC TGTTTGTGAC TCACTTCAAC TCCCTGGAAG TCATTGAGAT CCAGGCCAGA 
TCCTCACTGG GGAGCCCTGC CCGAGCATAT CTGGAAATTC CAAACCCTCG CTACCTGGGC 
CCCGCGATTT CCTCCGGAGC GATTTACCTG GCCTCCTCAT ACCAGGACAA GTTAAGGGTC 
ATATGCTGCA AAGGAAACCT CGTGAAGGAG TCAGGCACTG AGCAGCACCG GGTGCCCTCC 
ACCTCCCGCA GCAGCCCCAA CAAGCGAGGC CCACCAACAT ACAACGAGCA CATCACCAAA 
CGCGTGGCCT CCAGCCCGGC GCCACCGGAA GGCCCCAGCC ACCCCCGAGA GCCAAGCACA 
CCGCACCGCT ACCGAGACAG AGAGGGTCGG ACAGAGCTGC GCAGGGACAA GTCTCCAGGC 
CGCCCTCTGG AGCGGGAGAA GTCCCCAGGC CGAATGCTCA GCACTAGGAG AGAGCGGTCC 
CCAGGGAGAC TGTTTGAAGA CAGCAGCAGG GGCCGGCTGC CTGCAGGAGC AGTGAGGACC 
CCACTGTCCC AGGTTAACAA GGTCTGGGAC CAGTCTTCAG TATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017176134.1
CDS	1147..5991
Translation	MLLGEEAMMEQEMTRLHRRVSEVEAVLSQKEVELKASETQRSLLEQDLATYITECSSLKRSLEQARMEVSQEDDKALQLLHDIREQSRKLQEIKEQEYQAQVEEMRLMMNQLEEDLVSARRRSDLYESELRESRLAAEEFKRKANECQHKLMKVVSHPPRGDSGGTALDDLHKMQGHAGLTSAKDQGKPEVGEYSKLEKINAEQQLKIQELQEKLEKAVKASTEATELLQNIRQAKERAERELEKLHNREDSSEGIKKKLVEAEELEEKHREAQVSAQHLEVHLKQKEQHYEEKIKVLDNQIKKDLADKESLENMMQRHEEEAHEKGKILSEQKAMINAMDSKIRSLEQRIVELSEANKLAANSSLFTQRNMKAQEEMISELRQQKFYLETQAGKLEAQNRKLEEQLEKISHQDHSDKSRLLELETRLREVSLEHEEQKLELKRQLTELQLSLQERESQLTALQAARAALESQLRQAKTELEETTAEAEEEIQALTAHRDEIQRKFDALRNSCTVITDLEEQLNQLTEDNAELNNQNFYLSKQLDEASGANDEIVQLRSEVDHLRREITEREMQLTSQKQTMEALKTTCTMLEEQVLDLEALNDELLEKERQWEAWRSVLGDEKSQFECRVRELQRMLDTEKQSRARADQRITESRQVVELAVKEHKAEILALQQALKEQKLKAESLSDKLNDLEKKHAMLEMNARSLQQKLETERELKQRLLEEQAKLQQQMDLQKNHIFRLTQGLQEALDRADLLKTERSDLEYQLENIQVLYSHEKVKMEGTISQQTKLIDFLQAKMDQPAKKKKGLFSRRKEDPALPTQVPLQYNELKLALEKEKARCAELEEALQKTRIELRSAREEAAHRKATDHPHPSTPATARQQIAMSAIVRSPEHQPSAMSLLAPPSSRRKESSTPEEFSRRLKERMHHNIPHRFNVGLNMRATKCAVCLDTVHFGRQASKCLECQVMCHPKCSTCLPATCGLPAEYATHFTEAFCRDKMNSPGLQSKEPGSSLHLEGWMKVPRNNKRGQQGWDRKYIVLEGSKVLIYDNEAREAGQRPVEEFELCLPDGDVSIHGAVGASELANTAKADVPYILKMESHPHTTCWPGRTLYLLAPSFPDKQRWVTALESVVAGGRVSREKAEADAARDHTSSEHQPVWVEKLLGNSLLKLEGDDRLDMNCTLPFSDQVVLVGTEEGLYALNVLKNSLTHIPGIGAVFQIYIIKDLEKLLMIAGEERALCLVDVKKVKQSLAQSHLPAQPDVSPNIFEAVKGCHLFAAGKIENSLCICAAMPSKVVILRYNDNLSKYCIRKEIETSEPCSCIHFTNYSILIGTNKFYEIDMKQYTLDEFLDKNDHSLAPAVFASSSNSFPVSIVQANSAGQREEYLLCFHEFGVFVDSYGRRSRTDDLKWSRLPLAFAYREPYLFVTHFNSLEVIEIQARSSLGSPARAYLEIPNPRYLGPAISSGAIYLASSYQDKLRVICCKGNLVKESGTEQHRVPSTSRSSPNKRGPPTYNEHITKRVASSPAPPEGPSHPREPSTPHRYRDREGRTELRRDKSPGRPLEREKSPGRMLSTRRERSPGRLFEDSSRGRLPAGAVRTPLSQVNKVWDQSSV

Target ORF information:

RefSeq Version	XM_017320645.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus citron (Cit), transcript variant X29, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017320645.2

ORF Insert Sequence:

ATGCTCCTGG GCGAAGAAGC AATGATGGAG CAGGAAATGA CCCGGTTACA TCGCAGAGTG 
TCAGAGGTGG AGGCTGTGCT TAGTCAGAAG GAGGTGGAGC TGAAGGCCTC TGAGACTCAG 
AGATCCCTCC TGGAGCAGGA CCTTGCTACC TACATCACAG AATGCAGTAG CTTAAAGCGA 
AGTTTGGAGC AAGCGCGGAT GGAGGTGTCC CAGGAGGATG ACAAAGCTCT GCAGCTTCTC 
CACGACATCC GAGAGCAGAG CCGGAAGCTC CAGGAGATCA AGGAGCAGGA GTACCAGGCT 
CAGGTGGAGG AGATGAGGCT GATGATGAAT CAGCTGGAAG AAGACCTGGT GTCAGCCCGC 
AGACGCAGCG ATCTCTACGA GTCTGAGCTG AGGGAGTCTC GGCTTGCCGC CGAGGAATTC 
AAGCGGAAGG CAAACGAATG TCAGCACAAA CTGATGAAGG TAGTCAGCCA CCCTCCAAGA 
GGGGACTCCG GGGGCACGGC CCTGGATGAT CTTCACAAGA TGCAAGGCCA CGCTGGCTTA 
ACGAGTGCTA AGGACCAAGG GAAGCCTGAA GTGGGAGAAT ATTCCAAACT GGAGAAGATC 
AATGCTGAGC AGCAGCTGAA GATCCAGGAG CTCCAGGAGA AGCTGGAGAA GGCTGTAAAA 
GCCAGCACAG AGGCCACCGA GCTCCTGCAG AATATCCGCC AGGCAAAGGA GCGAGCAGAG 
CGGGAGCTAG AGAAGCTACA CAACCGGGAA GACTCCTCCG AGGGCATCAA AAAGAAGCTG 
GTGGAAGCCG AGGAGCTGGA GGAGAAACAT CGGGAGGCTC AGGTCTCAGC TCAACATCTA 
GAAGTACACT TGAAACAGAA AGAACAGCAC TACGAGGAAA AGATCAAGGT ATTGGACAAT 
CAGATAAAGA AGGACCTGGC CGACAAGGAG AGCCTGGAGA ACATGATGCA GAGACACGAG 
GAGGAGGCCC ACGAGAAGGG CAAGATCCTC AGCGAGCAGA AGGCGATGAT CAACGCGATG 
GATTCCAAGA TCCGATCCCT GGAGCAGAGG ATCGTGGAGC TGTCGGAAGC CAACAAGCTT 
GCGGCAAACA GCAGTCTCTT CACCCAGAGG AACATGAAGG CCCAGGAAGA GATGATCTCA 
GAACTCAGGC AGCAGAAATT TTACCTGGAG ACGCAGGCCG GAAAGCTGGA GGCCCAGAAC 
CGAAAGCTGG AAGAGCAACT GGAGAAGATC AGCCACCAAG ATCACAGTGA CAAGAGTCGG 
CTGCTGGAGC TGGAAACAAG GCTGAGGGAG GTCAGCCTGG AGCACGAGGA GCAGAAGCTG 
GAGCTGAAGC GGCAGCTCAC GGAGCTGCAG CTGTCCCTGC AGGAGCGCGA GTCCCAGCTG 
ACGGCCCTGC AGGCCGCCCG GGCAGCGCTG GAGAGCCAGC TCCGCCAGGC GAAGACAGAG 
CTGGAGGAGA CAACCGCGGA AGCGGAGGAG GAGATCCAGG CGCTCACGGC ACATCGAGAT 
GAGATCCAGC GCAAATTCGA TGCCCTTCGC AACAGCTGCA CGGTCATCAC CGACCTGGAG 
GAGCAGCTGA ACCAGCTCAC CGAGGACAAC GCCGAGCTCA ACAACCAAAA CTTCTACCTG 
TCCAAACAAC TCGATGAGGC TTCCGGGGCC AATGACGAGA TTGTGCAGCT GCGGAGTGAG 
GTGGACCATC TCCGCCGTGA GATCACGGAG CGGGAGATGC AGCTCACCAG CCAGAAGCAA 
ACGATGGAGG CTCTGAAGAC GACATGCACC ATGCTGGAGG AGCAGGTCCT GGACCTGGAG 
GCCCTGAACG ACGAGCTGCT GGAGAAGGAG CGCCAGTGGG AGGCCTGGCG GAGCGTCCTC 
GGCGACGAGA AGTCCCAGTT CGAGTGTCGA GTTCGAGAGC TACAGAGGAT GCTGGACACC 
GAGAAGCAGA GCAGGGCTAG GGCCGATCAG CGGATCACCG AGTCGCGCCA GGTGGTGGAG 
TTGGCGGTGA AGGAACACAA GGCCGAGATT CTTGCTCTGC AGCAGGCTCT CAAGGAGCAG 
AAGCTCAAAG CCGAGAGCCT GTCGGACAAG CTCAACGACC TGGAGAAGAA GCACGCCATG 
CTGGAGATGA ACGCCCGGAG CTTACAGCAG AAACTAGAGA CAGAGCGGGA GCTCAAACAG 
AGGCTTCTGG AGGAGCAAGC CAAATTGCAG CAGCAGATGG ACCTGCAGAA GAACCACATC 
TTCAGACTGA CGCAAGGGCT GCAGGAGGCG CTGGACCGGG CGGATCTGCT GAAGACAGAA 
AGGAGCGACC TGGAGTACCA GCTGGAAAAC ATTCAGGTTC TCTACTCTCA CGAGAAAGTG 
AAAATGGAAG GCACAATCTC TCAGCAAACC AAACTCATTG ATTTCCTGCA AGCCAAAATG 
GACCAGCCTG CTAAAAAGAA AAAGGGTTTA TTTAGTCGAC GGAAAGAGGA CCCTGCTTTG 
CCCACACAGG TTCCTCTGCA GTACAATGAG CTGAAGCTAG CCCTGGAAAA GGAGAAAGCC 
CGATGCGCGG AGCTGGAGGA GGCCCTTCAG AAGACCCGCA TCGAACTCCG CTCTGCGCGG 
GAGGAAGCTG CCCACCGCAA AGCCACGGAC CACCCGCACC CGTCTACGCC AGCCACTGCG 
AGGCAGCAGA TCGCCATGTC TGCCATTGTG CGGTCGCCCG AGCACCAGCC CAGTGCCATG 
AGCCTGCTTG CCCCACCATC CAGCCGCAGA AAGGAGTCGT CAACTCCAGA GGAATTCAGC 
CGGCGTCTGA AAGAGCGCAT GCACCACAAC ATCCCTCACC GGTTTAATGT GGGCCTGAAC 
ATGAGAGCCA CCAAGTGCGC CGTGTGTCTG GATACTGTGC ACTTTGGACG CCAGGCATCC 
AAATGCCTCG AATGTCAAGT GATGTGTCAC CCCAAATGCT CCACCTGCTT GCCCGCTACC 
TGTGGCCTGC CAGCTGAATA TGCCACACAC TTCACTGAGG CCTTCTGCCG GGACAAAATG 
AACTCCCCGG GGCTCCAGAG CAAGGAGCCT GGCAGCAGCT TGCACCTGGA AGGGTGGATG 
AAAGTGCCCA GAAATAACAA ACGGGGACAG CAAGGCTGGG ACAGGAAGTA CATTGTTCTG 
GAGGGGTCAA AAGTCCTCAT CTATGACAAT GAAGCCAGAG AAGCTGGACA GAGGCCGGTG 
GAAGAATTTG AGCTGTGCCT TCCTGACGGG GACGTATCTA TTCATGGTGC CGTTGGTGCT 
TCAGAACTTG CAAATACCGC CAAAGCAGAT GTCCCATACA TCCTGAAGAT GGAGTCTCAC 
CCACACACCA CCTGCTGGCC TGGGAGAACC CTCTACTTGC TAGCACCCAG CTTCCCCGAC 
AAGCAGCGCT GGGTCACCGC CTTAGAATCT GTCGTCGCAG GTGGGAGAGT TTCTAGGGAA 
AAGGCCGAAG CCGATGCTGC ACGTGACCAT ACTTCGAGTG AGCATCAGCC AGTGTGGGTT 
GAGAAATTAC TTGGAAACTC TCTGCTGAAA CTGGAGGGCG ATGACCGGCT TGACATGAAC 
TGCACCCTGC CCTTCAGTGA CCAGGTAGTG CTGGTGGGCA CGGAGGAAGG CCTCTACGCC 
CTGAATGTCT TGAAAAACTC CTTAACCCAC ATCCCAGGGA TTGGCGCAGT CTTCCAAATT 
TACATCATCA AGGACCTGGA GAAGCTGCTC ATGATAGCAG GGGAAGAGCG GGCTCTGTGC 
CTGGTGGACG TGAAGAAGGT GAAGCAGTCC CTGGCGCAGT CACACCTGCC TGCCCAGCCC 
GACGTCTCCC CCAACATATT CGAAGCCGTC AAAGGCTGCC ACTTGTTCGC TGCTGGCAAG 
ATCGAGAACA GCCTGTGCAT CTGCGCCGCT ATGCCAAGCA AAGTCGTCAT CCTCCGCTAC 
AATGACAACC TCAGCAAGTA CTGCATCCGC AAGGAGATCG AGACCTCAGA GCCCTGCAGC 
TGTATCCACT TCACCAACTA CAGCATCCTC ATTGGGACCA ACAAATTCTA TGAGATCGAC 
ATGAAGCAGT ACACGCTTGA CGAGTTCCTG GACAAGAACG ACCATTCCTT GGCACCTGCT 
GTGTTCGCCT CCTCGTCCAA CAGCTTCCCT GTCTCCATTG TGCAGGCGAA CAGCGCCGGG 
CAGCGAGAAG AATACCTGCT GTGCTTCCAC GAATTTGGGG TGTTCGTGGA TTCTTACGGA 
AGACGTAGCC GCACAGATGA TCTCAAGTGG AGTCGCTTAC CTCTGGCCTT CGCCTACAGA 
GAACCTTATC TGTTTGTGAC TCACTTCAAC TCCCTGGAAG TCATTGAGAT CCAGGCCAGA 
TCCTCACTGG GGAGCCCTGC CCGAGCATAT CTGGAAATTC CAAACCCTCG CTACCTGGGC 
CCCGCGATTT CCTCCGGAGC GATTTACCTG GCCTCCTCAT ACCAGGACAA GTTAAGGGTC 
ATATGCTGCA AAGGAAACCT CGTGAAGGAG TCAGGCACTG AGCAGCACCG GGTGCCCTCC 
ACCTCCCGCA GCAGCCCCAA CAAGCGAGGC CCACCAACAT ACAACGAGCA CATCACCAAA 
CGCGTGGCCT CCAGCCCGGC GCCACCGGAA GGCCCCAGCC ACCCCCGAGA GCCAAGCACA 
CCGCACCGCT ACCGAGACAG AGAGGGTCGG ACAGAGCTGC GCAGGGACAA GTCTCCAGGC 
CGCCCTCTGG AGCGGGAGAA GTCCCCAGGC CGAATGCTCA GCACTAGGAG AGAGCGGTCC 
CCAGGGAGAC TGTTTGAAGA CAGCAGCAGG GGCCGGCTGC CTGCAGGAGC AGTGAGGACC 
CCACTGTCCC AGGTTAACAA GGTCTGGGAC CAGTCTTCAG TATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cit ( XM_017320646.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017176135.1 Mus musculus citron (Cit), transcript variant X30, mRNA.

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CloneID	OMu81237
Clone ID Related Accession (Same CDS sequence)	XM_017320646.2 , XM_017320646.1
Accession Version	XM_017320646.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4839bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	citron Rho-interacting kinase isoform X25
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000071.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_017320646.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTCCTGG GCGAAGAAGC AATGATGGAG CAGGAAATGA CCCGGTTACA TCGCAGAGTG 
TCAGAGGTGG AGGCTGTGCT TAGTCAGAAG GAGGTGGAGC TGAAGGCCTC TGAGACTCAG 
AGATCCCTCC TGGAGCAGGA CCTTGCTACC TACATCACAG AATGCAGTAG CTTAAAGCGA 
AGTTTGGAGC AAGCGCGGAT GGAGGTGTCC CAGGAGGATG ACAAAGCTCT GCAGCTTCTC 
CACGACATCC GAGAGCAGAG CCGGAAGCTC CAGGAGATCA AGGAGCAGGA GTACCAGGCT 
CAGGTGGAGG AGATGAGGCT GATGATGAAT CAGCTGGAAG AAGACCTGGT GTCAGCCCGC 
AGACGCAGCG ATCTCTACGA GTCTGAGCTG AGGGAGTCTC GGCTTGCCGC CGAGGAATTC 
AAGCGGAAGG CAAACGAATG TCAGCACAAA CTGATGAAGG CTAAGGACCA AGGGAAGCCT 
GAAGTGGGAG AATATTCCAA ACTGGAGAAG ATCAATGCTG AGCAGCAGCT GAAGATCCAG 
GAGCTCCAGG AGAAGCTGGA GAAGGCTGTA AAAGCCAGCA CAGAGGCCAC CGAGCTCCTG 
CAGAATATCC GCCAGGCAAA GGAGCGAGCA GAGCGGGAGC TAGAGAAGCT ACACAACCGG 
GAAGACTCCT CCGAGGGCAT CAAAAAGAAG CTGGTGGAAG CCGAGGAACG CCGCCACTCC 
CTGGAGAACA AGGTAAAGAG ACTAGAGACC ATGGAGCGTA GAGAGAACAG ACTGAAGGAT 
GACATCCAGA CAAAGTCCGA ACAGATCCAG CAGATGGCTG ATAAAATTCT GGAGCTGGAG 
GAGAAACATC GGGAGGCTCA GGTCTCAGCT CAACATCTAG AAGTACACTT GAAACAGAAA 
GAACAGCACT ACGAGGAAAA GATCAAGGTA TTGGACAATC AGATAAAGAA GGACCTGGCC 
GACAAGGAGA GCCTGGAGAA CATGATGCAG AGACACGAGG AGGAGGCCCA CGAGAAGGGC 
AAGATCCTCA GCGAGCAGAA GGCGATGATC AACGCGATGG ATTCCAAGAT CCGATCCCTG 
GAGCAGAGGA TCGTGGAGCT GTCGGAAGCC AACAAGCTTG CGGCAAACAG CAGTCTCTTC 
ACCCAGAGGA ACATGAAGGC CCAGGAAGAG ATGATCTCAG AACTCAGGCA GCAGAAATTT 
TACCTGGAGA CGCAGGCCGG AAAGCTGGAG GCCCAGAACC GAAAGCTGGA AGAGCAACTG 
GAGAAGATCA GCCACCAAGA TCACAGTGAC AAGAGTCGGC TGCTGGAGCT GGAAACAAGG 
CTGAGGGAGG TCAGCCTGGA GCACGAGGAG CAGAAGCTGG AGCTGAAGCG GCAGCTCACG 
GAGCTGCAGC TGTCCCTGCA GGAGCGCGAG TCCCAGCTGA CGGCCCTGCA GGCCGCCCGG 
GCAGCGCTGG AGAGCCAGCT CCGCCAGGCG AAGACAGAGC TGGAGGAGAC AACCGCGGAA 
GCGGAGGAGG AGATCCAGGC GCTCACGGCA CATCGAGATG AGATCCAGCG CAAATTCGAT 
GCCCTTCGCA ACAGCTGCAC GGTCATCACC GACCTGGAGG AGCAGCTGAA CCAGCTCACC 
GAGGACAACG CCGAGCTCAA CAACCAAAAC TTCTACCTGT CCAAACAACT CGATGAGGCT 
TCCGGGGCCA ATGACGAGAT TGTGCAGCTG CGGAGTGAGG TGGACCATCT CCGCCGTGAG 
ATCACGGAGC GGGAGATGCA GCTCACCAGC CAGAAGCAAA CGATGGAGGC TCTGAAGACG 
ACATGCACCA TGCTGGAGGA GCAGGTCCTG GACCTGGAGG CCCTGAACGA CGAGCTGCTG 
GAGAAGGAGC GCCAGTGGGA GGCCTGGCGG AGCGTCCTCG GCGACGAGAA GTCCCAGTTC 
GAGTGTCGAG TTCGAGAGCT ACAGAGGATG CTGGACACCG AGAAGCAGAG CAGGGCTAGG 
GCCGATCAGC GGATCACCGA GTCGCGCCAG GTGGTGGAGT TGGCGGTGAA GGAACACAAG 
GCCGAGATTC TTGCTCTGCA GCAGGCTCTC AAGGAGCAGA AGCTCAAAGC CGAGAGCCTG 
TCGGACAAGC TCAACGACCT GGAGAAGAAG CACGCCATGC TGGAGATGAA CGCCCGGAGC 
TTACAGCAGA AACTAGAGAC AGAGCGGGAG CTCAAACAGA GGCTTCTGGA GGAGCAAGCC 
AAATTGCAGC AGCAGATGGA CCTGCAGAAG AACCACATCT TCAGACTGAC GCAAGGGCTG 
CAGGAGGCGC TGGACCGGGC GGATCTGCTG AAGACAGAAA GGAGCGACCT GGAGTACCAG 
CTGGAAAACA TTCAGGTTCT CTACTCTCAC GAGAAAGTGA AAATGGAAGG CACAATCTCT 
CAGCAAACCA AACTCATTGA TTTCCTGCAA GCCAAAATGG ACCAGCCTGC TAAAAAGAAA 
AAGGTTCCTC TGCAGTACAA TGAGCTGAAG CTAGCCCTGG AAAAGGAGAA AGCCCGATGC 
GCGGAGCTGG AGGAGGCCCT TCAGAAGACC CGCATCGAAC TCCGCTCTGC GCGGGAGGAA 
GCTGCCCACC GCAAAGCCAC GGACCACCCG CACCCGTCTA CGCCAGCCAC TGCGAGGCAG 
CAGATCGCCA TGTCTGCCAT TGTGCGGTCG CCCGAGCACC AGCCCAGTGC CATGAGCCTG 
CTTGCCCCAC CATCCAGCCG CAGAAAGGAG TCGTCAACTC CAGAGGAATT CAGCCGGCGT 
CTGAAAGAGC GCATGCACCA CAACATCCCT CACCGGTTTA ATGTGGGCCT GAACATGAGA 
GCCACCAAGT GCGCCGTGTG TCTGGATACT GTGCACTTTG GACGCCAGGC ATCCAAATGC 
CTCGAATGTC AAGTGATGTG TCACCCCAAA TGCTCCACCT GCTTGCCCGC TACCTGTGGC 
CTGCCAGCTG AATATGCCAC ACACTTCACT GAGGCCTTCT GCCGGGACAA AATGAACTCC 
CCGGGGCTCC AGAGCAAGGA GCCTGGCAGC AGCTTGCACC TGGAAGGGTG GATGAAAGTG 
CCCAGAAATA ACAAACGGGG ACAGCAAGGC TGGGACAGGA AGTACATTGT TCTGGAGGGG 
TCAAAAGTCC TCATCTATGA CAATGAAGCC AGAGAAGCTG GACAGAGGCC GGTGGAAGAA 
TTTGAGCTGT GCCTTCCTGA CGGGGACGTA TCTATTCATG GTGCCGTTGG TGCTTCAGAA 
CTTGCAAATA CCGCCAAAGC AGATGTCCCA TACATCCTGA AGATGGAGTC TCACCCACAC 
ACCACCTGCT GGCCTGGGAG AACCCTCTAC TTGCTAGCAC CCAGCTTCCC CGACAAGCAG 
CGCTGGGTCA CCGCCTTAGA ATCTGTCGTC GCAGGTGGGA GAGTTTCTAG GGAAAAGGCC 
GAAGCCGATG CTGCACGTGA CCATACTTCG AGTGAGCATC AGCCAGTGTG GGTTGAGAAA 
TTACTTGGAA ACTCTCTGCT GAAACTGGAG GGCGATGACC GGCTTGACAT GAACTGCACC 
CTGCCCTTCA GTGACCAGGT AGTGCTGGTG GGCACGGAGG AAGGCCTCTA CGCCCTGAAT 
GTCTTGAAAA ACTCCTTAAC CCACATCCCA GGGATTGGCG CAGTCTTCCA AATTTACATC 
ATCAAGGACC TGGAGAAGCT GCTCATGATA GCAGGGGAAG AGCGGGCTCT GTGCCTGGTG 
GACGTGAAGA AGGTGAAGCA GTCCCTGGCG CAGTCACACC TGCCTGCCCA GCCCGACGTC 
TCCCCCAACA TATTCGAAGC CGTCAAAGGC TGCCACTTGT TCGCTGCTGG CAAGATCGAG 
AACAGCCTGT GCATCTGCGC CGCTATGCCA AGCAAAGTCG TCATCCTCCG CTACAATGAC 
AACCTCAGCA AGTACTGCAT CCGCAAGGAG ATCGAGACCT CAGAGCCCTG CAGCTGTATC 
CACTTCACCA ACTACAGCAT CCTCATTGGG ACCAACAAAT TCTATGAGAT CGACATGAAG 
CAGTACACGC TTGACGAGTT CCTGGACAAG AACGACCATT CCTTGGCACC TGCTGTGTTC 
GCCTCCTCGT CCAACAGCTT CCCTGTCTCC ATTGTGCAGG CGAACAGCGC CGGGCAGCGA 
GAAGAATACC TGCTGTGCTT CCACGAATTT GGGGTGTTCG TGGATTCTTA CGGAAGACGT 
AGCCGCACAG ATGATCTCAA GTGGAGTCGC TTACCTCTGG CCTTCGCCTA CAGAGAACCT 
TATCTGTTTG TGACTCACTT CAACTCCCTG GAAGTCATTG AGATCCAGGC CAGATCCTCA 
CTGGGGAGCC CTGCCCGAGC ATATCTGGAA ATTCCAAACC CTCGCTACCT GGGCCCCGCG 
ATTTCCTCCG GAGCGATTTA CCTGGCCTCC TCATACCAGG ACAAGTTAAG GGTCATATGC 
TGCAAAGGAA ACCTCGTGAA GGAGTCAGGC ACTGAGCAGC ACCGGGTGCC CTCCACCTCC 
CGCAGCAGCC CCAACAAGCG AGGCCCACCA ACATACAACG AGCACATCAC CAAACGCGTG 
GCCTCCAGCC CGGCGCCACC GGAAGGCCCC AGCCACCCCC GAGAGCCAAG CACACCGCAC 
CGCTACCGAG ACAGAGAGGG TCGGACAGAG CTGCGCAGGG ACAAGTCTCC AGGCCGCCCT 
CTGGAGCGGG AGAAGTCCCC AGGCCGAATG CTCAGCACTA GGAGAGAGCG GTCCCCAGGG 
AGACTGTTTG AAGACAGCAG CAGGGGCCGG CTGCCTGCAG GAGCAGTGAG GACCCCACTG 
TCCCAGGTTA ACAAGGTCTG GGACCAGTCT TCAGTATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017176135.1
CDS	1147..5985
Translation	MLLGEEAMMEQEMTRLHRRVSEVEAVLSQKEVELKASETQRSLLEQDLATYITECSSLKRSLEQARMEVSQEDDKALQLLHDIREQSRKLQEIKEQEYQAQVEEMRLMMNQLEEDLVSARRRSDLYESELRESRLAAEEFKRKANECQHKLMKAKDQGKPEVGEYSKLEKINAEQQLKIQELQEKLEKAVKASTEATELLQNIRQAKERAERELEKLHNREDSSEGIKKKLVEAEERRHSLENKVKRLETMERRENRLKDDIQTKSEQIQQMADKILELEEKHREAQVSAQHLEVHLKQKEQHYEEKIKVLDNQIKKDLADKESLENMMQRHEEEAHEKGKILSEQKAMINAMDSKIRSLEQRIVELSEANKLAANSSLFTQRNMKAQEEMISELRQQKFYLETQAGKLEAQNRKLEEQLEKISHQDHSDKSRLLELETRLREVSLEHEEQKLELKRQLTELQLSLQERESQLTALQAARAALESQLRQAKTELEETTAEAEEEIQALTAHRDEIQRKFDALRNSCTVITDLEEQLNQLTEDNAELNNQNFYLSKQLDEASGANDEIVQLRSEVDHLRREITEREMQLTSQKQTMEALKTTCTMLEEQVLDLEALNDELLEKERQWEAWRSVLGDEKSQFECRVRELQRMLDTEKQSRARADQRITESRQVVELAVKEHKAEILALQQALKEQKLKAESLSDKLNDLEKKHAMLEMNARSLQQKLETERELKQRLLEEQAKLQQQMDLQKNHIFRLTQGLQEALDRADLLKTERSDLEYQLENIQVLYSHEKVKMEGTISQQTKLIDFLQAKMDQPAKKKKVPLQYNELKLALEKEKARCAELEEALQKTRIELRSAREEAAHRKATDHPHPSTPATARQQIAMSAIVRSPEHQPSAMSLLAPPSSRRKESSTPEEFSRRLKERMHHNIPHRFNVGLNMRATKCAVCLDTVHFGRQASKCLECQVMCHPKCSTCLPATCGLPAEYATHFTEAFCRDKMNSPGLQSKEPGSSLHLEGWMKVPRNNKRGQQGWDRKYIVLEGSKVLIYDNEAREAGQRPVEEFELCLPDGDVSIHGAVGASELANTAKADVPYILKMESHPHTTCWPGRTLYLLAPSFPDKQRWVTALESVVAGGRVSREKAEADAARDHTSSEHQPVWVEKLLGNSLLKLEGDDRLDMNCTLPFSDQVVLVGTEEGLYALNVLKNSLTHIPGIGAVFQIYIIKDLEKLLMIAGEERALCLVDVKKVKQSLAQSHLPAQPDVSPNIFEAVKGCHLFAAGKIENSLCICAAMPSKVVILRYNDNLSKYCIRKEIETSEPCSCIHFTNYSILIGTNKFYEIDMKQYTLDEFLDKNDHSLAPAVFASSSNSFPVSIVQANSAGQREEYLLCFHEFGVFVDSYGRRSRTDDLKWSRLPLAFAYREPYLFVTHFNSLEVIEIQARSSLGSPARAYLEIPNPRYLGPAISSGAIYLASSYQDKLRVICCKGNLVKESGTEQHRVPSTSRSSPNKRGPPTYNEHITKRVASSPAPPEGPSHPREPSTPHRYRDREGRTELRRDKSPGRPLEREKSPGRMLSTRRERSPGRLFEDSSRGRLPAGAVRTPLSQVNKVWDQSSV

Target ORF information:

RefSeq Version	XM_017320646.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus citron (Cit), transcript variant X30, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017320646.2

ORF Insert Sequence:

ATGCTCCTGG GCGAAGAAGC AATGATGGAG CAGGAAATGA CCCGGTTACA TCGCAGAGTG 
TCAGAGGTGG AGGCTGTGCT TAGTCAGAAG GAGGTGGAGC TGAAGGCCTC TGAGACTCAG 
AGATCCCTCC TGGAGCAGGA CCTTGCTACC TACATCACAG AATGCAGTAG CTTAAAGCGA 
AGTTTGGAGC AAGCGCGGAT GGAGGTGTCC CAGGAGGATG ACAAAGCTCT GCAGCTTCTC 
CACGACATCC GAGAGCAGAG CCGGAAGCTC CAGGAGATCA AGGAGCAGGA GTACCAGGCT 
CAGGTGGAGG AGATGAGGCT GATGATGAAT CAGCTGGAAG AAGACCTGGT GTCAGCCCGC 
AGACGCAGCG ATCTCTACGA GTCTGAGCTG AGGGAGTCTC GGCTTGCCGC CGAGGAATTC 
AAGCGGAAGG CAAACGAATG TCAGCACAAA CTGATGAAGG CTAAGGACCA AGGGAAGCCT 
GAAGTGGGAG AATATTCCAA ACTGGAGAAG ATCAATGCTG AGCAGCAGCT GAAGATCCAG 
GAGCTCCAGG AGAAGCTGGA GAAGGCTGTA AAAGCCAGCA CAGAGGCCAC CGAGCTCCTG 
CAGAATATCC GCCAGGCAAA GGAGCGAGCA GAGCGGGAGC TAGAGAAGCT ACACAACCGG 
GAAGACTCCT CCGAGGGCAT CAAAAAGAAG CTGGTGGAAG CCGAGGAACG CCGCCACTCC 
CTGGAGAACA AGGTAAAGAG ACTAGAGACC ATGGAGCGTA GAGAGAACAG ACTGAAGGAT 
GACATCCAGA CAAAGTCCGA ACAGATCCAG CAGATGGCTG ATAAAATTCT GGAGCTGGAG 
GAGAAACATC GGGAGGCTCA GGTCTCAGCT CAACATCTAG AAGTACACTT GAAACAGAAA 
GAACAGCACT ACGAGGAAAA GATCAAGGTA TTGGACAATC AGATAAAGAA GGACCTGGCC 
GACAAGGAGA GCCTGGAGAA CATGATGCAG AGACACGAGG AGGAGGCCCA CGAGAAGGGC 
AAGATCCTCA GCGAGCAGAA GGCGATGATC AACGCGATGG ATTCCAAGAT CCGATCCCTG 
GAGCAGAGGA TCGTGGAGCT GTCGGAAGCC AACAAGCTTG CGGCAAACAG CAGTCTCTTC 
ACCCAGAGGA ACATGAAGGC CCAGGAAGAG ATGATCTCAG AACTCAGGCA GCAGAAATTT 
TACCTGGAGA CGCAGGCCGG AAAGCTGGAG GCCCAGAACC GAAAGCTGGA AGAGCAACTG 
GAGAAGATCA GCCACCAAGA TCACAGTGAC AAGAGTCGGC TGCTGGAGCT GGAAACAAGG 
CTGAGGGAGG TCAGCCTGGA GCACGAGGAG CAGAAGCTGG AGCTGAAGCG GCAGCTCACG 
GAGCTGCAGC TGTCCCTGCA GGAGCGCGAG TCCCAGCTGA CGGCCCTGCA GGCCGCCCGG 
GCAGCGCTGG AGAGCCAGCT CCGCCAGGCG AAGACAGAGC TGGAGGAGAC AACCGCGGAA 
GCGGAGGAGG AGATCCAGGC GCTCACGGCA CATCGAGATG AGATCCAGCG CAAATTCGAT 
GCCCTTCGCA ACAGCTGCAC GGTCATCACC GACCTGGAGG AGCAGCTGAA CCAGCTCACC 
GAGGACAACG CCGAGCTCAA CAACCAAAAC TTCTACCTGT CCAAACAACT CGATGAGGCT 
TCCGGGGCCA ATGACGAGAT TGTGCAGCTG CGGAGTGAGG TGGACCATCT CCGCCGTGAG 
ATCACGGAGC GGGAGATGCA GCTCACCAGC CAGAAGCAAA CGATGGAGGC TCTGAAGACG 
ACATGCACCA TGCTGGAGGA GCAGGTCCTG GACCTGGAGG CCCTGAACGA CGAGCTGCTG 
GAGAAGGAGC GCCAGTGGGA GGCCTGGCGG AGCGTCCTCG GCGACGAGAA GTCCCAGTTC 
GAGTGTCGAG TTCGAGAGCT ACAGAGGATG CTGGACACCG AGAAGCAGAG CAGGGCTAGG 
GCCGATCAGC GGATCACCGA GTCGCGCCAG GTGGTGGAGT TGGCGGTGAA GGAACACAAG 
GCCGAGATTC TTGCTCTGCA GCAGGCTCTC AAGGAGCAGA AGCTCAAAGC CGAGAGCCTG 
TCGGACAAGC TCAACGACCT GGAGAAGAAG CACGCCATGC TGGAGATGAA CGCCCGGAGC 
TTACAGCAGA AACTAGAGAC AGAGCGGGAG CTCAAACAGA GGCTTCTGGA GGAGCAAGCC 
AAATTGCAGC AGCAGATGGA CCTGCAGAAG AACCACATCT TCAGACTGAC GCAAGGGCTG 
CAGGAGGCGC TGGACCGGGC GGATCTGCTG AAGACAGAAA GGAGCGACCT GGAGTACCAG 
CTGGAAAACA TTCAGGTTCT CTACTCTCAC GAGAAAGTGA AAATGGAAGG CACAATCTCT 
CAGCAAACCA AACTCATTGA TTTCCTGCAA GCCAAAATGG ACCAGCCTGC TAAAAAGAAA 
AAGGTTCCTC TGCAGTACAA TGAGCTGAAG CTAGCCCTGG AAAAGGAGAA AGCCCGATGC 
GCGGAGCTGG AGGAGGCCCT TCAGAAGACC CGCATCGAAC TCCGCTCTGC GCGGGAGGAA 
GCTGCCCACC GCAAAGCCAC GGACCACCCG CACCCGTCTA CGCCAGCCAC TGCGAGGCAG 
CAGATCGCCA TGTCTGCCAT TGTGCGGTCG CCCGAGCACC AGCCCAGTGC CATGAGCCTG 
CTTGCCCCAC CATCCAGCCG CAGAAAGGAG TCGTCAACTC CAGAGGAATT CAGCCGGCGT 
CTGAAAGAGC GCATGCACCA CAACATCCCT CACCGGTTTA ATGTGGGCCT GAACATGAGA 
GCCACCAAGT GCGCCGTGTG TCTGGATACT GTGCACTTTG GACGCCAGGC ATCCAAATGC 
CTCGAATGTC AAGTGATGTG TCACCCCAAA TGCTCCACCT GCTTGCCCGC TACCTGTGGC 
CTGCCAGCTG AATATGCCAC ACACTTCACT GAGGCCTTCT GCCGGGACAA AATGAACTCC 
CCGGGGCTCC AGAGCAAGGA GCCTGGCAGC AGCTTGCACC TGGAAGGGTG GATGAAAGTG 
CCCAGAAATA ACAAACGGGG ACAGCAAGGC TGGGACAGGA AGTACATTGT TCTGGAGGGG 
TCAAAAGTCC TCATCTATGA CAATGAAGCC AGAGAAGCTG GACAGAGGCC GGTGGAAGAA 
TTTGAGCTGT GCCTTCCTGA CGGGGACGTA TCTATTCATG GTGCCGTTGG TGCTTCAGAA 
CTTGCAAATA CCGCCAAAGC AGATGTCCCA TACATCCTGA AGATGGAGTC TCACCCACAC 
ACCACCTGCT GGCCTGGGAG AACCCTCTAC TTGCTAGCAC CCAGCTTCCC CGACAAGCAG 
CGCTGGGTCA CCGCCTTAGA ATCTGTCGTC GCAGGTGGGA GAGTTTCTAG GGAAAAGGCC 
GAAGCCGATG CTGCACGTGA CCATACTTCG AGTGAGCATC AGCCAGTGTG GGTTGAGAAA 
TTACTTGGAA ACTCTCTGCT GAAACTGGAG GGCGATGACC GGCTTGACAT GAACTGCACC 
CTGCCCTTCA GTGACCAGGT AGTGCTGGTG GGCACGGAGG AAGGCCTCTA CGCCCTGAAT 
GTCTTGAAAA ACTCCTTAAC CCACATCCCA GGGATTGGCG CAGTCTTCCA AATTTACATC 
ATCAAGGACC TGGAGAAGCT GCTCATGATA GCAGGGGAAG AGCGGGCTCT GTGCCTGGTG 
GACGTGAAGA AGGTGAAGCA GTCCCTGGCG CAGTCACACC TGCCTGCCCA GCCCGACGTC 
TCCCCCAACA TATTCGAAGC CGTCAAAGGC TGCCACTTGT TCGCTGCTGG CAAGATCGAG 
AACAGCCTGT GCATCTGCGC CGCTATGCCA AGCAAAGTCG TCATCCTCCG CTACAATGAC 
AACCTCAGCA AGTACTGCAT CCGCAAGGAG ATCGAGACCT CAGAGCCCTG CAGCTGTATC 
CACTTCACCA ACTACAGCAT CCTCATTGGG ACCAACAAAT TCTATGAGAT CGACATGAAG 
CAGTACACGC TTGACGAGTT CCTGGACAAG AACGACCATT CCTTGGCACC TGCTGTGTTC 
GCCTCCTCGT CCAACAGCTT CCCTGTCTCC ATTGTGCAGG CGAACAGCGC CGGGCAGCGA 
GAAGAATACC TGCTGTGCTT CCACGAATTT GGGGTGTTCG TGGATTCTTA CGGAAGACGT 
AGCCGCACAG ATGATCTCAA GTGGAGTCGC TTACCTCTGG CCTTCGCCTA CAGAGAACCT 
TATCTGTTTG TGACTCACTT CAACTCCCTG GAAGTCATTG AGATCCAGGC CAGATCCTCA 
CTGGGGAGCC CTGCCCGAGC ATATCTGGAA ATTCCAAACC CTCGCTACCT GGGCCCCGCG 
ATTTCCTCCG GAGCGATTTA CCTGGCCTCC TCATACCAGG ACAAGTTAAG GGTCATATGC 
TGCAAAGGAA ACCTCGTGAA GGAGTCAGGC ACTGAGCAGC ACCGGGTGCC CTCCACCTCC 
CGCAGCAGCC CCAACAAGCG AGGCCCACCA ACATACAACG AGCACATCAC CAAACGCGTG 
GCCTCCAGCC CGGCGCCACC GGAAGGCCCC AGCCACCCCC GAGAGCCAAG CACACCGCAC 
CGCTACCGAG ACAGAGAGGG TCGGACAGAG CTGCGCAGGG ACAAGTCTCC AGGCCGCCCT 
CTGGAGCGGG AGAAGTCCCC AGGCCGAATG CTCAGCACTA GGAGAGAGCG GTCCCCAGGG 
AGACTGTTTG AAGACAGCAG CAGGGGCCGG CTGCCTGCAG GAGCAGTGAG GACCCCACTG 
TCCCAGGTTA ACAAGGTCTG GGACCAGTCT TCAGTATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cit ( XM_017320646.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017176135.1 Mus musculus citron (Cit), transcript variant X28, mRNA.

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CloneID	OMu81237
Clone ID Related Accession (Same CDS sequence)	XM_017320646.2 , XM_017320646.1
Accession Version	XM_017320646.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4839bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	citron Rho-interacting kinase isoform X24
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000071.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTCCTGG GCGAAGAAGC AATGATGGAG CAGGAAATGA CCCGGTTACA TCGCAGAGTG 
TCAGAGGTGG AGGCTGTGCT TAGTCAGAAG GAGGTGGAGC TGAAGGCCTC TGAGACTCAG 
AGATCCCTCC TGGAGCAGGA CCTTGCTACC TACATCACAG AATGCAGTAG CTTAAAGCGA 
AGTTTGGAGC AAGCGCGGAT GGAGGTGTCC CAGGAGGATG ACAAAGCTCT GCAGCTTCTC 
CACGACATCC GAGAGCAGAG CCGGAAGCTC CAGGAGATCA AGGAGCAGGA GTACCAGGCT 
CAGGTGGAGG AGATGAGGCT GATGATGAAT CAGCTGGAAG AAGACCTGGT GTCAGCCCGC 
AGACGCAGCG ATCTCTACGA GTCTGAGCTG AGGGAGTCTC GGCTTGCCGC CGAGGAATTC 
AAGCGGAAGG CAAACGAATG TCAGCACAAA CTGATGAAGG CTAAGGACCA AGGGAAGCCT 
GAAGTGGGAG AATATTCCAA ACTGGAGAAG ATCAATGCTG AGCAGCAGCT GAAGATCCAG 
GAGCTCCAGG AGAAGCTGGA GAAGGCTGTA AAAGCCAGCA CAGAGGCCAC CGAGCTCCTG 
CAGAATATCC GCCAGGCAAA GGAGCGAGCA GAGCGGGAGC TAGAGAAGCT ACACAACCGG 
GAAGACTCCT CCGAGGGCAT CAAAAAGAAG CTGGTGGAAG CCGAGGAACG CCGCCACTCC 
CTGGAGAACA AGGTAAAGAG ACTAGAGACC ATGGAGCGTA GAGAGAACAG ACTGAAGGAT 
GACATCCAGA CAAAGTCCGA ACAGATCCAG CAGATGGCTG ATAAAATTCT GGAGCTGGAG 
GAGAAACATC GGGAGGCTCA GGTCTCAGCT CAACATCTAG AAGTACACTT GAAACAGAAA 
GAACAGCACT ACGAGGAAAA GATCAAGGTA TTGGACAATC AGATAAAGAA GGACCTGGCC 
GACAAGGAGA GCCTGGAGAA CATGATGCAG AGACACGAGG AGGAGGCCCA CGAGAAGGGC 
AAGATCCTCA GCGAGCAGAA GGCGATGATC AACGCGATGG ATTCCAAGAT CCGATCCCTG 
GAGCAGAGGA TCGTGGAGCT GTCGGAAGCC AACAAGCTTG CGGCAAACAG CAGTCTCTTC 
ACCCAGAGGA ACATGAAGGC CCAGGAAGAG ATGATCTCAG AACTCAGGCA GCAGAAATTT 
TACCTGGAGA CGCAGGCCGG AAAGCTGGAG GCCCAGAACC GAAAGCTGGA AGAGCAACTG 
GAGAAGATCA GCCACCAAGA TCACAGTGAC AAGAGTCGGC TGCTGGAGCT GGAAACAAGG 
CTGAGGGAGG TCAGCCTGGA GCACGAGGAG CAGAAGCTGG AGCTGAAGCG GCAGCTCACG 
GAGCTGCAGC TGTCCCTGCA GGAGCGCGAG TCCCAGCTGA CGGCCCTGCA GGCCGCCCGG 
GCAGCGCTGG AGAGCCAGCT CCGCCAGGCG AAGACAGAGC TGGAGGAGAC AACCGCGGAA 
GCGGAGGAGG AGATCCAGGC GCTCACGGCA CATCGAGATG AGATCCAGCG CAAATTCGAT 
GCCCTTCGCA ACAGCTGCAC GGTCATCACC GACCTGGAGG AGCAGCTGAA CCAGCTCACC 
GAGGACAACG CCGAGCTCAA CAACCAAAAC TTCTACCTGT CCAAACAACT CGATGAGGCT 
TCCGGGGCCA ATGACGAGAT TGTGCAGCTG CGGAGTGAGG TGGACCATCT CCGCCGTGAG 
ATCACGGAGC GGGAGATGCA GCTCACCAGC CAGAAGCAAA CGATGGAGGC TCTGAAGACG 
ACATGCACCA TGCTGGAGGA GCAGGTCCTG GACCTGGAGG CCCTGAACGA CGAGCTGCTG 
GAGAAGGAGC GCCAGTGGGA GGCCTGGCGG AGCGTCCTCG GCGACGAGAA GTCCCAGTTC 
GAGTGTCGAG TTCGAGAGCT ACAGAGGATG CTGGACACCG AGAAGCAGAG CAGGGCTAGG 
GCCGATCAGC GGATCACCGA GTCGCGCCAG GTGGTGGAGT TGGCGGTGAA GGAACACAAG 
GCCGAGATTC TTGCTCTGCA GCAGGCTCTC AAGGAGCAGA AGCTCAAAGC CGAGAGCCTG 
TCGGACAAGC TCAACGACCT GGAGAAGAAG CACGCCATGC TGGAGATGAA CGCCCGGAGC 
TTACAGCAGA AACTAGAGAC AGAGCGGGAG CTCAAACAGA GGCTTCTGGA GGAGCAAGCC 
AAATTGCAGC AGCAGATGGA CCTGCAGAAG AACCACATCT TCAGACTGAC GCAAGGGCTG 
CAGGAGGCGC TGGACCGGGC GGATCTGCTG AAGACAGAAA GGAGCGACCT GGAGTACCAG 
CTGGAAAACA TTCAGGTTCT CTACTCTCAC GAGAAAGTGA AAATGGAAGG CACAATCTCT 
CAGCAAACCA AACTCATTGA TTTCCTGCAA GCCAAAATGG ACCAGCCTGC TAAAAAGAAA 
AAGGTTCCTC TGCAGTACAA TGAGCTGAAG CTAGCCCTGG AAAAGGAGAA AGCCCGATGC 
GCGGAGCTGG AGGAGGCCCT TCAGAAGACC CGCATCGAAC TCCGCTCTGC GCGGGAGGAA 
GCTGCCCACC GCAAAGCCAC GGACCACCCG CACCCGTCTA CGCCAGCCAC TGCGAGGCAG 
CAGATCGCCA TGTCTGCCAT TGTGCGGTCG CCCGAGCACC AGCCCAGTGC CATGAGCCTG 
CTTGCCCCAC CATCCAGCCG CAGAAAGGAG TCGTCAACTC CAGAGGAATT CAGCCGGCGT 
CTGAAAGAGC GCATGCACCA CAACATCCCT CACCGGTTTA ATGTGGGCCT GAACATGAGA 
GCCACCAAGT GCGCCGTGTG TCTGGATACT GTGCACTTTG GACGCCAGGC ATCCAAATGC 
CTCGAATGTC AAGTGATGTG TCACCCCAAA TGCTCCACCT GCTTGCCCGC TACCTGTGGC 
CTGCCAGCTG AATATGCCAC ACACTTCACT GAGGCCTTCT GCCGGGACAA AATGAACTCC 
CCGGGGCTCC AGAGCAAGGA GCCTGGCAGC AGCTTGCACC TGGAAGGGTG GATGAAAGTG 
CCCAGAAATA ACAAACGGGG ACAGCAAGGC TGGGACAGGA AGTACATTGT TCTGGAGGGG 
TCAAAAGTCC TCATCTATGA CAATGAAGCC AGAGAAGCTG GACAGAGGCC GGTGGAAGAA 
TTTGAGCTGT GCCTTCCTGA CGGGGACGTA TCTATTCATG GTGCCGTTGG TGCTTCAGAA 
CTTGCAAATA CCGCCAAAGC AGATGTCCCA TACATCCTGA AGATGGAGTC TCACCCACAC 
ACCACCTGCT GGCCTGGGAG AACCCTCTAC TTGCTAGCAC CCAGCTTCCC CGACAAGCAG 
CGCTGGGTCA CCGCCTTAGA ATCTGTCGTC GCAGGTGGGA GAGTTTCTAG GGAAAAGGCC 
GAAGCCGATG CTGCACGTGA CCATACTTCG AGTGAGCATC AGCCAGTGTG GGTTGAGAAA 
TTACTTGGAA ACTCTCTGCT GAAACTGGAG GGCGATGACC GGCTTGACAT GAACTGCACC 
CTGCCCTTCA GTGACCAGGT AGTGCTGGTG GGCACGGAGG AAGGCCTCTA CGCCCTGAAT 
GTCTTGAAAA ACTCCTTAAC CCACATCCCA GGGATTGGCG CAGTCTTCCA AATTTACATC 
ATCAAGGACC TGGAGAAGCT GCTCATGATA GCAGGGGAAG AGCGGGCTCT GTGCCTGGTG 
GACGTGAAGA AGGTGAAGCA GTCCCTGGCG CAGTCACACC TGCCTGCCCA GCCCGACGTC 
TCCCCCAACA TATTCGAAGC CGTCAAAGGC TGCCACTTGT TCGCTGCTGG CAAGATCGAG 
AACAGCCTGT GCATCTGCGC CGCTATGCCA AGCAAAGTCG TCATCCTCCG CTACAATGAC 
AACCTCAGCA AGTACTGCAT CCGCAAGGAG ATCGAGACCT CAGAGCCCTG CAGCTGTATC 
CACTTCACCA ACTACAGCAT CCTCATTGGG ACCAACAAAT TCTATGAGAT CGACATGAAG 
CAGTACACGC TTGACGAGTT CCTGGACAAG AACGACCATT CCTTGGCACC TGCTGTGTTC 
GCCTCCTCGT CCAACAGCTT CCCTGTCTCC ATTGTGCAGG CGAACAGCGC CGGGCAGCGA 
GAAGAATACC TGCTGTGCTT CCACGAATTT GGGGTGTTCG TGGATTCTTA CGGAAGACGT 
AGCCGCACAG ATGATCTCAA GTGGAGTCGC TTACCTCTGG CCTTCGCCTA CAGAGAACCT 
TATCTGTTTG TGACTCACTT CAACTCCCTG GAAGTCATTG AGATCCAGGC CAGATCCTCA 
CTGGGGAGCC CTGCCCGAGC ATATCTGGAA ATTCCAAACC CTCGCTACCT GGGCCCCGCG 
ATTTCCTCCG GAGCGATTTA CCTGGCCTCC TCATACCAGG ACAAGTTAAG GGTCATATGC 
TGCAAAGGAA ACCTCGTGAA GGAGTCAGGC ACTGAGCAGC ACCGGGTGCC CTCCACCTCC 
CGCAGCAGCC CCAACAAGCG AGGCCCACCA ACATACAACG AGCACATCAC CAAACGCGTG 
GCCTCCAGCC CGGCGCCACC GGAAGGCCCC AGCCACCCCC GAGAGCCAAG CACACCGCAC 
CGCTACCGAG ACAGAGAGGG TCGGACAGAG CTGCGCAGGG ACAAGTCTCC AGGCCGCCCT 
CTGGAGCGGG AGAAGTCCCC AGGCCGAATG CTCAGCACTA GGAGAGAGCG GTCCCCAGGG 
AGACTGTTTG AAGACAGCAG CAGGGGCCGG CTGCCTGCAG GAGCAGTGAG GACCCCACTG 
TCCCAGGTTA ACAAGGTCTG GGACCAGTCT TCAGTATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017176135.1
CDS	1419..6257
Translation	MLLGEEAMMEQEMTRLHRRVSEVEAVLSQKEVELKASETQRSLLEQDLATYITECSSLKRSLEQARMEVSQEDDKALQLLHDIREQSRKLQEIKEQEYQAQVEEMRLMMNQLEEDLVSARRRSDLYESELRESRLAAEEFKRKANECQHKLMKAKDQGKPEVGEYSKLEKINAEQQLKIQELQEKLEKAVKASTEATELLQNIRQAKERAERELEKLHNREDSSEGIKKKLVEAEERRHSLENKVKRLETMERRENRLKDDIQTKSEQIQQMADKILELEEKHREAQVSAQHLEVHLKQKEQHYEEKIKVLDNQIKKDLADKESLENMMQRHEEEAHEKGKILSEQKAMINAMDSKIRSLEQRIVELSEANKLAANSSLFTQRNMKAQEEMISELRQQKFYLETQAGKLEAQNRKLEEQLEKISHQDHSDKSRLLELETRLREVSLEHEEQKLELKRQLTELQLSLQERESQLTALQAARAALESQLRQAKTELEETTAEAEEEIQALTAHRDEIQRKFDALRNSCTVITDLEEQLNQLTEDNAELNNQNFYLSKQLDEASGANDEIVQLRSEVDHLRREITEREMQLTSQKQTMEALKTTCTMLEEQVLDLEALNDELLEKERQWEAWRSVLGDEKSQFECRVRELQRMLDTEKQSRARADQRITESRQVVELAVKEHKAEILALQQALKEQKLKAESLSDKLNDLEKKHAMLEMNARSLQQKLETERELKQRLLEEQAKLQQQMDLQKNHIFRLTQGLQEALDRADLLKTERSDLEYQLENIQVLYSHEKVKMEGTISQQTKLIDFLQAKMDQPAKKKKVPLQYNELKLALEKEKARCAELEEALQKTRIELRSAREEAAHRKATDHPHPSTPATARQQIAMSAIVRSPEHQPSAMSLLAPPSSRRKESSTPEEFSRRLKERMHHNIPHRFNVGLNMRATKCAVCLDTVHFGRQASKCLECQVMCHPKCSTCLPATCGLPAEYATHFTEAFCRDKMNSPGLQSKEPGSSLHLEGWMKVPRNNKRGQQGWDRKYIVLEGSKVLIYDNEAREAGQRPVEEFELCLPDGDVSIHGAVGASELANTAKADVPYILKMESHPHTTCWPGRTLYLLAPSFPDKQRWVTALESVVAGGRVSREKAEADAARDHTSSEHQPVWVEKLLGNSLLKLEGDDRLDMNCTLPFSDQVVLVGTEEGLYALNVLKNSLTHIPGIGAVFQIYIIKDLEKLLMIAGEERALCLVDVKKVKQSLAQSHLPAQPDVSPNIFEAVKGCHLFAAGKIENSLCICAAMPSKVVILRYNDNLSKYCIRKEIETSEPCSCIHFTNYSILIGTNKFYEIDMKQYTLDEFLDKNDHSLAPAVFASSSNSFPVSIVQANSAGQREEYLLCFHEFGVFVDSYGRRSRTDDLKWSRLPLAFAYREPYLFVTHFNSLEVIEIQARSSLGSPARAYLEIPNPRYLGPAISSGAIYLASSYQDKLRVICCKGNLVKESGTEQHRVPSTSRSSPNKRGPPTYNEHITKRVASSPAPPEGPSHPREPSTPHRYRDREGRTELRRDKSPGRPLEREKSPGRMLSTRRERSPGRLFEDSSRGRLPAGAVRTPLSQVNKVWDQSSV

Target ORF information:

RefSeq Version	XM_017320646.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus citron (Cit), transcript variant X28, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017320646.1

ORF Insert Sequence:

ATGCTCCTGG GCGAAGAAGC AATGATGGAG CAGGAAATGA CCCGGTTACA TCGCAGAGTG 
TCAGAGGTGG AGGCTGTGCT TAGTCAGAAG GAGGTGGAGC TGAAGGCCTC TGAGACTCAG 
AGATCCCTCC TGGAGCAGGA CCTTGCTACC TACATCACAG AATGCAGTAG CTTAAAGCGA 
AGTTTGGAGC AAGCGCGGAT GGAGGTGTCC CAGGAGGATG ACAAAGCTCT GCAGCTTCTC 
CACGACATCC GAGAGCAGAG CCGGAAGCTC CAGGAGATCA AGGAGCAGGA GTACCAGGCT 
CAGGTGGAGG AGATGAGGCT GATGATGAAT CAGCTGGAAG AAGACCTGGT GTCAGCCCGC 
AGACGCAGCG ATCTCTACGA GTCTGAGCTG AGGGAGTCTC GGCTTGCCGC CGAGGAATTC 
AAGCGGAAGG CAAACGAATG TCAGCACAAA CTGATGAAGG CTAAGGACCA AGGGAAGCCT 
GAAGTGGGAG AATATTCCAA ACTGGAGAAG ATCAATGCTG AGCAGCAGCT GAAGATCCAG 
GAGCTCCAGG AGAAGCTGGA GAAGGCTGTA AAAGCCAGCA CAGAGGCCAC CGAGCTCCTG 
CAGAATATCC GCCAGGCAAA GGAGCGAGCA GAGCGGGAGC TAGAGAAGCT ACACAACCGG 
GAAGACTCCT CCGAGGGCAT CAAAAAGAAG CTGGTGGAAG CCGAGGAACG CCGCCACTCC 
CTGGAGAACA AGGTAAAGAG ACTAGAGACC ATGGAGCGTA GAGAGAACAG ACTGAAGGAT 
GACATCCAGA CAAAGTCCGA ACAGATCCAG CAGATGGCTG ATAAAATTCT GGAGCTGGAG 
GAGAAACATC GGGAGGCTCA GGTCTCAGCT CAACATCTAG AAGTACACTT GAAACAGAAA 
GAACAGCACT ACGAGGAAAA GATCAAGGTA TTGGACAATC AGATAAAGAA GGACCTGGCC 
GACAAGGAGA GCCTGGAGAA CATGATGCAG AGACACGAGG AGGAGGCCCA CGAGAAGGGC 
AAGATCCTCA GCGAGCAGAA GGCGATGATC AACGCGATGG ATTCCAAGAT CCGATCCCTG 
GAGCAGAGGA TCGTGGAGCT GTCGGAAGCC AACAAGCTTG CGGCAAACAG CAGTCTCTTC 
ACCCAGAGGA ACATGAAGGC CCAGGAAGAG ATGATCTCAG AACTCAGGCA GCAGAAATTT 
TACCTGGAGA CGCAGGCCGG AAAGCTGGAG GCCCAGAACC GAAAGCTGGA AGAGCAACTG 
GAGAAGATCA GCCACCAAGA TCACAGTGAC AAGAGTCGGC TGCTGGAGCT GGAAACAAGG 
CTGAGGGAGG TCAGCCTGGA GCACGAGGAG CAGAAGCTGG AGCTGAAGCG GCAGCTCACG 
GAGCTGCAGC TGTCCCTGCA GGAGCGCGAG TCCCAGCTGA CGGCCCTGCA GGCCGCCCGG 
GCAGCGCTGG AGAGCCAGCT CCGCCAGGCG AAGACAGAGC TGGAGGAGAC AACCGCGGAA 
GCGGAGGAGG AGATCCAGGC GCTCACGGCA CATCGAGATG AGATCCAGCG CAAATTCGAT 
GCCCTTCGCA ACAGCTGCAC GGTCATCACC GACCTGGAGG AGCAGCTGAA CCAGCTCACC 
GAGGACAACG CCGAGCTCAA CAACCAAAAC TTCTACCTGT CCAAACAACT CGATGAGGCT 
TCCGGGGCCA ATGACGAGAT TGTGCAGCTG CGGAGTGAGG TGGACCATCT CCGCCGTGAG 
ATCACGGAGC GGGAGATGCA GCTCACCAGC CAGAAGCAAA CGATGGAGGC TCTGAAGACG 
ACATGCACCA TGCTGGAGGA GCAGGTCCTG GACCTGGAGG CCCTGAACGA CGAGCTGCTG 
GAGAAGGAGC GCCAGTGGGA GGCCTGGCGG AGCGTCCTCG GCGACGAGAA GTCCCAGTTC 
GAGTGTCGAG TTCGAGAGCT ACAGAGGATG CTGGACACCG AGAAGCAGAG CAGGGCTAGG 
GCCGATCAGC GGATCACCGA GTCGCGCCAG GTGGTGGAGT TGGCGGTGAA GGAACACAAG 
GCCGAGATTC TTGCTCTGCA GCAGGCTCTC AAGGAGCAGA AGCTCAAAGC CGAGAGCCTG 
TCGGACAAGC TCAACGACCT GGAGAAGAAG CACGCCATGC TGGAGATGAA CGCCCGGAGC 
TTACAGCAGA AACTAGAGAC AGAGCGGGAG CTCAAACAGA GGCTTCTGGA GGAGCAAGCC 
AAATTGCAGC AGCAGATGGA CCTGCAGAAG AACCACATCT TCAGACTGAC GCAAGGGCTG 
CAGGAGGCGC TGGACCGGGC GGATCTGCTG AAGACAGAAA GGAGCGACCT GGAGTACCAG 
CTGGAAAACA TTCAGGTTCT CTACTCTCAC GAGAAAGTGA AAATGGAAGG CACAATCTCT 
CAGCAAACCA AACTCATTGA TTTCCTGCAA GCCAAAATGG ACCAGCCTGC TAAAAAGAAA 
AAGGTTCCTC TGCAGTACAA TGAGCTGAAG CTAGCCCTGG AAAAGGAGAA AGCCCGATGC 
GCGGAGCTGG AGGAGGCCCT TCAGAAGACC CGCATCGAAC TCCGCTCTGC GCGGGAGGAA 
GCTGCCCACC GCAAAGCCAC GGACCACCCG CACCCGTCTA CGCCAGCCAC TGCGAGGCAG 
CAGATCGCCA TGTCTGCCAT TGTGCGGTCG CCCGAGCACC AGCCCAGTGC CATGAGCCTG 
CTTGCCCCAC CATCCAGCCG CAGAAAGGAG TCGTCAACTC CAGAGGAATT CAGCCGGCGT 
CTGAAAGAGC GCATGCACCA CAACATCCCT CACCGGTTTA ATGTGGGCCT GAACATGAGA 
GCCACCAAGT GCGCCGTGTG TCTGGATACT GTGCACTTTG GACGCCAGGC ATCCAAATGC 
CTCGAATGTC AAGTGATGTG TCACCCCAAA TGCTCCACCT GCTTGCCCGC TACCTGTGGC 
CTGCCAGCTG AATATGCCAC ACACTTCACT GAGGCCTTCT GCCGGGACAA AATGAACTCC 
CCGGGGCTCC AGAGCAAGGA GCCTGGCAGC AGCTTGCACC TGGAAGGGTG GATGAAAGTG 
CCCAGAAATA ACAAACGGGG ACAGCAAGGC TGGGACAGGA AGTACATTGT TCTGGAGGGG 
TCAAAAGTCC TCATCTATGA CAATGAAGCC AGAGAAGCTG GACAGAGGCC GGTGGAAGAA 
TTTGAGCTGT GCCTTCCTGA CGGGGACGTA TCTATTCATG GTGCCGTTGG TGCTTCAGAA 
CTTGCAAATA CCGCCAAAGC AGATGTCCCA TACATCCTGA AGATGGAGTC TCACCCACAC 
ACCACCTGCT GGCCTGGGAG AACCCTCTAC TTGCTAGCAC CCAGCTTCCC CGACAAGCAG 
CGCTGGGTCA CCGCCTTAGA ATCTGTCGTC GCAGGTGGGA GAGTTTCTAG GGAAAAGGCC 
GAAGCCGATG CTGCACGTGA CCATACTTCG AGTGAGCATC AGCCAGTGTG GGTTGAGAAA 
TTACTTGGAA ACTCTCTGCT GAAACTGGAG GGCGATGACC GGCTTGACAT GAACTGCACC 
CTGCCCTTCA GTGACCAGGT AGTGCTGGTG GGCACGGAGG AAGGCCTCTA CGCCCTGAAT 
GTCTTGAAAA ACTCCTTAAC CCACATCCCA GGGATTGGCG CAGTCTTCCA AATTTACATC 
ATCAAGGACC TGGAGAAGCT GCTCATGATA GCAGGGGAAG AGCGGGCTCT GTGCCTGGTG 
GACGTGAAGA AGGTGAAGCA GTCCCTGGCG CAGTCACACC TGCCTGCCCA GCCCGACGTC 
TCCCCCAACA TATTCGAAGC CGTCAAAGGC TGCCACTTGT TCGCTGCTGG CAAGATCGAG 
AACAGCCTGT GCATCTGCGC CGCTATGCCA AGCAAAGTCG TCATCCTCCG CTACAATGAC 
AACCTCAGCA AGTACTGCAT CCGCAAGGAG ATCGAGACCT CAGAGCCCTG CAGCTGTATC 
CACTTCACCA ACTACAGCAT CCTCATTGGG ACCAACAAAT TCTATGAGAT CGACATGAAG 
CAGTACACGC TTGACGAGTT CCTGGACAAG AACGACCATT CCTTGGCACC TGCTGTGTTC 
GCCTCCTCGT CCAACAGCTT CCCTGTCTCC ATTGTGCAGG CGAACAGCGC CGGGCAGCGA 
GAAGAATACC TGCTGTGCTT CCACGAATTT GGGGTGTTCG TGGATTCTTA CGGAAGACGT 
AGCCGCACAG ATGATCTCAA GTGGAGTCGC TTACCTCTGG CCTTCGCCTA CAGAGAACCT 
TATCTGTTTG TGACTCACTT CAACTCCCTG GAAGTCATTG AGATCCAGGC CAGATCCTCA 
CTGGGGAGCC CTGCCCGAGC ATATCTGGAA ATTCCAAACC CTCGCTACCT GGGCCCCGCG 
ATTTCCTCCG GAGCGATTTA CCTGGCCTCC TCATACCAGG ACAAGTTAAG GGTCATATGC 
TGCAAAGGAA ACCTCGTGAA GGAGTCAGGC ACTGAGCAGC ACCGGGTGCC CTCCACCTCC 
CGCAGCAGCC CCAACAAGCG AGGCCCACCA ACATACAACG AGCACATCAC CAAACGCGTG 
GCCTCCAGCC CGGCGCCACC GGAAGGCCCC AGCCACCCCC GAGAGCCAAG CACACCGCAC 
CGCTACCGAG ACAGAGAGGG TCGGACAGAG CTGCGCAGGG ACAAGTCTCC AGGCCGCCCT 
CTGGAGCGGG AGAAGTCCCC AGGCCGAATG CTCAGCACTA GGAGAGAGCG GTCCCCAGGG 
AGACTGTTTG AAGACAGCAG CAGGGGCCGG CTGCCTGCAG GAGCAGTGAG GACCCCACTG 
TCCCAGGTTA ACAAGGTCTG GGACCAGTCT TCAGTATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cit ( XM_017320647.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017176136.1 Mus musculus citron (Cit), transcript variant X29, mRNA.

Price & Availability↑

CloneID	OMu81238
Clone ID Related Accession (Same CDS sequence)	XM_017320647.1
Accession Version	XM_017320647.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4821bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	citron Rho-interacting kinase isoform X25
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000071.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTCCTGG GCGAAGAAGC AATGATGGAG CAGGAAATGA CCCGGTTACA TCGCAGAGTG 
TCAGAGGTGG AGGCTGTGCT TAGTCAGAAG GAGGTGGAGC TGAAGGCCTC TGAGACTCAG 
AGATCCCTCC TGGAGCAGGA CCTTGCTACC TACATCACAG AATGCAGTAG CTTAAAGCGA 
AGTTTGGAGC AAGCGCGGAT GGAGGTGTCC CAGGAGGATG ACAAAGCTCT GCAGCTTCTC 
CACGACATCC GAGAGCAGAG CCGGAAGCTC CAGGAGATCA AGGAGCAGGA GTACCAGGCT 
CAGGTGGAGG AGATGAGGCT GATGATGAAT CAGCTGGAAG AAGACCTGGT GTCAGCCCGC 
AGACGCAGCG ATCTCTACGA GTCTGAGCTG AGGGAGTCTC GGCTTGCCGC CGAGGAATTC 
AAGCGGAAGG CAAACGAATG TCAGCACAAA CTGATGAAGG CTAAGGACCA AGGGAAGCCT 
GAAGTGGGAG AATATTCCAA ACTGGAGAAG ATCAATGCTG AGCAGCAGCT GAAGATCCAG 
GAGCTCCAGG AGAAGCTGGA GAAGGCTGTA AAAGCCAGCA CAGAGGCCAC CGAGCTCCTG 
CAGAATATCC GCCAGGCAAA GGAGCGAGCA GAGCGGGAGC TAGAGAAGCT ACACAACCGG 
GAAGACTCCT CCGAGGGCAT CAAAAAGAAG CTGGTGGAAG CCGAGGAACG CCGCCACTCC 
CTGGAGAACA AGGTAAAGAG ACTAGAGACC ATGGAGCGTA GAGAGAACAG ACTGAAGGAT 
GACATCCAGA CAAAGTCCGA ACAGATCCAG CAGATGGCTG ATAAAATTCT GGAGCTGGAG 
GAGAAACATC GGGAGGCTCA GGTCTCAGCT CAACATCTAG AAGTACACTT GAAACAGAAA 
GAACAGCACT ACGAGGAAAA GATCAAGGTA TTGGACAATC AGATAAAGAA GGACCTGGCC 
GACAAGGAGA GCCTGGAGAA CATGATGCAG AGACACGAGG AGGAGGCCCA CGAGAAGGGC 
AAGATCCTCA GCGAGCAGAA GGCGATGATC AACGCGATGG ATTCCAAGAT CCGATCCCTG 
GAGCAGAGGA TCGTGGAGCT GTCGGAAGCC AACAAGCTTG CGGCAAACAG CAGTCTCTTC 
ACCCAGAGGA ACATGAAGGC CCAGGAAGAG ATGATCTCAG AACTCAGGCA GCAGAAATTT 
TACCTGGAGA CGCAGGCCGG AAAGCTGGAG GCCCAGAACC GAAAGCTGGA AGAGCAACTG 
GAGAAGATCA GCCACCAAGA TCACAGTGAC AAGAGTCGGC TGCTGGAGCT GGAAACAAGG 
CTGAGGGAGG TCAGCCTGGA GCACGAGGAG CAGAAGCTGG AGCTGAAGCG GCAGCTCACG 
GAGCTGCAGC TGTCCCTGCA GGAGCGCGAG TCCCAGCTGA CGGCCCTGCA GGCCGCCCGG 
GCAGCGCTGG AGAGCCAGCT CCGCCAGGCG AAGACAGAGC TGGAGGAGAC AACCGCGGAA 
GCGGAGGAGG AGATCCAGGC GCTCACGGCA CATCGAGATG AGATCCAGCG CAAATTCGAT 
GCCCTTCGCA ACAGCTGCAC GGTCATCACC GACCTGGAGG AGCAGCTGAA CCAGCTCACC 
GAGGACAACG CCGAGCTCAA CAACCAAAAC TTCTACCTGT CCAAACAACT CGATGAGGCT 
TCCGGGGCCA ATGACGAGAT TGTGCAGCTG CGGAGTGAGG TGGACCATCT CCGCCGTGAG 
ATCACGGAGC GGGAGATGCA GCTCACCAGC CAGAAGCAAG CACAACTCAG TGCTCCTGAT 
TTGCAGACGA TGGAGGCTCT GAAGACGACA TGCACCATGC TGGAGGAGCA GGTCCTGGAC 
CTGGAGGCCC TGAACGACGA GCTGCTGGAG AAGGAGCGCC AGTGGGAGGC CTGGCGGAGC 
GTCCTCGGCG ACGAGAAGTC CCAGTTCGAG TGTCGAGTTC GAGAGCTACA GAGGATGCTG 
GACACCGAGA AGCAGAGCAG GGCTAGGGCC GATCAGCGGA TCACCGAGTC GCGCCAGGTG 
GTGGAGTTGG CGGTGAAGGA ACACAAGGCC GAGATTCTTG CTCTGCAGCA GGCTCTCAAG 
GAGCAGAAGC TCAAAGCCGA GAGCCTGTCG GACAAGCTCA ACGACCTGGA GAAGAAGCAC 
GCCATGCTGG AGATGAACGC CCGGAGCTTA CAGCAGAAAC TAGAGACAGA GCGGGAGCTC 
AAACAGAGGC TTCTGGAGGA GCAAGCCAAA TTGCAGCAGC AGATGGACCT GCAGAAGAAC 
CACATCTTCA GACTGACGCA AGGGCTGCAG GAGGCGCTGG ACCGGGCGGA TCTGCTGAAG 
ACAGAAAGGA GCGACCTGGA GTACCAGCTG GAAAACATTC AGGTTCTCTA CTCTCACGAG 
AAAGTGAAAA TGGAAGGCAC AATCTCTCAG CAAACCAAAC TCATTGATTT CCTGCAAGCC 
AAAATGGACC AGCCTGCTAA AAAGAAAAAG GTTCCTCTGC AGTACAATGA GCTGAAGCTA 
GCCCTGGAAA AGGAGAAAGC CCGATGCGCG GAGCTGGAGG AGGCCCTTCA GAAGACCCGC 
ATCGAACTCC GCTCTGCGCG GGAGGAAGCT GCCCACCGCA AAGCCACGGA CCACCCGCAC 
CCGTCTACGC CAGCCACTGC GAGGCAGCAG ATCGCCATGT CTGCCATTGT GCGGTCGCCC 
GAGCACCAGC CCAGTGCCAT GAGCCTGCTT GCCCCACCAT CCAGCCGCAG AAAGGAGTCG 
TCAACTCCAG AGGAATTCAG CCGGCGTCTG AAAGAGCGCA TGCACCACAA CATCCCTCAC 
CGGTTTAATG TGGGCCTGAA CATGAGAGCC ACCAAGTGCG CCGTGTGTCT GGATACTGTG 
CACTTTGGAC GCCAGGCATC CAAATGCCTC GAATGTCAAG TGATGTGTCA CCCCAAATGC 
TCCACCTGCT TGCCCGCTAC CTGTGGCCTG CCAGCTGAAT ATGCCACACA CTTCACTGAG 
GCCTTCTGCC GGGACAAAAT GAACTCCCCG GGGCTCCAGA GCAAGGAGCC TGGCAGCAGC 
TTGCACCTGG AAGGGTGGAT GAAAGTGCCC AGAAATAACA AACGGGGACA GCAAGGCTGG 
GACAGGAAGT ACATTGTTCT GGAGGGGTCA AAAGTCCTCA TCTATGACAA TGAAGCCAGA 
GAAGCTGGAC AGAGGCCGGT GGAAGAATTT GAGCTGTGCC TTCCTGACGG GGACGTATCT 
ATTCATGGTG CCGTTGGTGC TTCAGAACTT GCAAATACCG CCAAAGCAGA TGTCCCATAC 
ATCCTGAAGA TGGAGTCTCA CCCACACACC ACCTGCTGGC CTGGGAGAAC CCTCTACTTG 
CTAGCACCCA GCTTCCCCGA CAAGCAGCGC TGGGTCACCG CCTTAGAATC TGTCGTCGCA 
GGTGGGAGAG TTTCTAGGGA AAAGGCCGAA GCCGATGCTA AATTACTTGG AAACTCTCTG 
CTGAAACTGG AGGGCGATGA CCGGCTTGAC ATGAACTGCA CCCTGCCCTT CAGTGACCAG 
GTAGTGCTGG TGGGCACGGA GGAAGGCCTC TACGCCCTGA ATGTCTTGAA AAACTCCTTA 
ACCCACATCC CAGGGATTGG CGCAGTCTTC CAAATTTACA TCATCAAGGA CCTGGAGAAG 
CTGCTCATGA TAGCAGGGGA AGAGCGGGCT CTGTGCCTGG TGGACGTGAA GAAGGTGAAG 
CAGTCCCTGG CGCAGTCACA CCTGCCTGCC CAGCCCGACG TCTCCCCCAA CATATTCGAA 
GCCGTCAAAG GCTGCCACTT GTTCGCTGCT GGCAAGATCG AGAACAGCCT GTGCATCTGC 
GCCGCTATGC CAAGCAAAGT CGTCATCCTC CGCTACAATG ACAACCTCAG CAAGTACTGC 
ATCCGCAAGG AGATCGAGAC CTCAGAGCCC TGCAGCTGTA TCCACTTCAC CAACTACAGC 
ATCCTCATTG GGACCAACAA ATTCTATGAG ATCGACATGA AGCAGTACAC GCTTGACGAG 
TTCCTGGACA AGAACGACCA TTCCTTGGCA CCTGCTGTGT TCGCCTCCTC GTCCAACAGC 
TTCCCTGTCT CCATTGTGCA GGCGAACAGC GCCGGGCAGC GAGAAGAATA CCTGCTGTGC 
TTCCACGAAT TTGGGGTGTT CGTGGATTCT TACGGAAGAC GTAGCCGCAC AGATGATCTC 
AAGTGGAGTC GCTTACCTCT GGCCTTCGCC TACAGAGAAC CTTATCTGTT TGTGACTCAC 
TTCAACTCCC TGGAAGTCAT TGAGATCCAG GCCAGATCCT CACTGGGGAG CCCTGCCCGA 
GCATATCTGG AAATTCCAAA CCCTCGCTAC CTGGGCCCCG CGATTTCCTC CGGAGCGATT 
TACCTGGCCT CCTCATACCA GGACAAGTTA AGGGTCATAT GCTGCAAAGG AAACCTCGTG 
AAGGAGTCAG GCACTGAGCA GCACCGGGTG CCCTCCACCT CCCGCAGCAG CCCCAACAAG 
CGAGGCCCAC CAACATACAA CGAGCACATC ACCAAACGCG TGGCCTCCAG CCCGGCGCCA 
CCGGAAGGCC CCAGCCACCC CCGAGAGCCA AGCACACCGC ACCGCTACCG AGACAGAGAG 
GGTCGGACAG AGCTGCGCAG GGACAAGTCT CCAGGCCGCC CTCTGGAGCG GGAGAAGTCC 
CCAGGCCGAA TGCTCAGCAC TAGGAGAGAG CGGTCCCCAG GGAGACTGTT TGAAGACAGC 
AGCAGGGGCC GGCTGCCTGC AGGAGCAGTG AGGACCCCAC TGTCCCAGGT TAACAAGGTC 
TGGGACCAGT CTTCAGTATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017176136.1
CDS	1419..6239
Translation	MLLGEEAMMEQEMTRLHRRVSEVEAVLSQKEVELKASETQRSLLEQDLATYITECSSLKRSLEQARMEVSQEDDKALQLLHDIREQSRKLQEIKEQEYQAQVEEMRLMMNQLEEDLVSARRRSDLYESELRESRLAAEEFKRKANECQHKLMKAKDQGKPEVGEYSKLEKINAEQQLKIQELQEKLEKAVKASTEATELLQNIRQAKERAERELEKLHNREDSSEGIKKKLVEAEERRHSLENKVKRLETMERRENRLKDDIQTKSEQIQQMADKILELEEKHREAQVSAQHLEVHLKQKEQHYEEKIKVLDNQIKKDLADKESLENMMQRHEEEAHEKGKILSEQKAMINAMDSKIRSLEQRIVELSEANKLAANSSLFTQRNMKAQEEMISELRQQKFYLETQAGKLEAQNRKLEEQLEKISHQDHSDKSRLLELETRLREVSLEHEEQKLELKRQLTELQLSLQERESQLTALQAARAALESQLRQAKTELEETTAEAEEEIQALTAHRDEIQRKFDALRNSCTVITDLEEQLNQLTEDNAELNNQNFYLSKQLDEASGANDEIVQLRSEVDHLRREITEREMQLTSQKQAQLSAPDLQTMEALKTTCTMLEEQVLDLEALNDELLEKERQWEAWRSVLGDEKSQFECRVRELQRMLDTEKQSRARADQRITESRQVVELAVKEHKAEILALQQALKEQKLKAESLSDKLNDLEKKHAMLEMNARSLQQKLETERELKQRLLEEQAKLQQQMDLQKNHIFRLTQGLQEALDRADLLKTERSDLEYQLENIQVLYSHEKVKMEGTISQQTKLIDFLQAKMDQPAKKKKVPLQYNELKLALEKEKARCAELEEALQKTRIELRSAREEAAHRKATDHPHPSTPATARQQIAMSAIVRSPEHQPSAMSLLAPPSSRRKESSTPEEFSRRLKERMHHNIPHRFNVGLNMRATKCAVCLDTVHFGRQASKCLECQVMCHPKCSTCLPATCGLPAEYATHFTEAFCRDKMNSPGLQSKEPGSSLHLEGWMKVPRNNKRGQQGWDRKYIVLEGSKVLIYDNEAREAGQRPVEEFELCLPDGDVSIHGAVGASELANTAKADVPYILKMESHPHTTCWPGRTLYLLAPSFPDKQRWVTALESVVAGGRVSREKAEADAKLLGNSLLKLEGDDRLDMNCTLPFSDQVVLVGTEEGLYALNVLKNSLTHIPGIGAVFQIYIIKDLEKLLMIAGEERALCLVDVKKVKQSLAQSHLPAQPDVSPNIFEAVKGCHLFAAGKIENSLCICAAMPSKVVILRYNDNLSKYCIRKEIETSEPCSCIHFTNYSILIGTNKFYEIDMKQYTLDEFLDKNDHSLAPAVFASSSNSFPVSIVQANSAGQREEYLLCFHEFGVFVDSYGRRSRTDDLKWSRLPLAFAYREPYLFVTHFNSLEVIEIQARSSLGSPARAYLEIPNPRYLGPAISSGAIYLASSYQDKLRVICCKGNLVKESGTEQHRVPSTSRSSPNKRGPPTYNEHITKRVASSPAPPEGPSHPREPSTPHRYRDREGRTELRRDKSPGRPLEREKSPGRMLSTRRERSPGRLFEDSSRGRLPAGAVRTPLSQVNKVWDQSSV

Target ORF information:

RefSeq Version	XM_017320647.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus citron (Cit), transcript variant X29, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017320647.1

ORF Insert Sequence:

ATGCTCCTGG GCGAAGAAGC AATGATGGAG CAGGAAATGA CCCGGTTACA TCGCAGAGTG 
TCAGAGGTGG AGGCTGTGCT TAGTCAGAAG GAGGTGGAGC TGAAGGCCTC TGAGACTCAG 
AGATCCCTCC TGGAGCAGGA CCTTGCTACC TACATCACAG AATGCAGTAG CTTAAAGCGA 
AGTTTGGAGC AAGCGCGGAT GGAGGTGTCC CAGGAGGATG ACAAAGCTCT GCAGCTTCTC 
CACGACATCC GAGAGCAGAG CCGGAAGCTC CAGGAGATCA AGGAGCAGGA GTACCAGGCT 
CAGGTGGAGG AGATGAGGCT GATGATGAAT CAGCTGGAAG AAGACCTGGT GTCAGCCCGC 
AGACGCAGCG ATCTCTACGA GTCTGAGCTG AGGGAGTCTC GGCTTGCCGC CGAGGAATTC 
AAGCGGAAGG CAAACGAATG TCAGCACAAA CTGATGAAGG CTAAGGACCA AGGGAAGCCT 
GAAGTGGGAG AATATTCCAA ACTGGAGAAG ATCAATGCTG AGCAGCAGCT GAAGATCCAG 
GAGCTCCAGG AGAAGCTGGA GAAGGCTGTA AAAGCCAGCA CAGAGGCCAC CGAGCTCCTG 
CAGAATATCC GCCAGGCAAA GGAGCGAGCA GAGCGGGAGC TAGAGAAGCT ACACAACCGG 
GAAGACTCCT CCGAGGGCAT CAAAAAGAAG CTGGTGGAAG CCGAGGAACG CCGCCACTCC 
CTGGAGAACA AGGTAAAGAG ACTAGAGACC ATGGAGCGTA GAGAGAACAG ACTGAAGGAT 
GACATCCAGA CAAAGTCCGA ACAGATCCAG CAGATGGCTG ATAAAATTCT GGAGCTGGAG 
GAGAAACATC GGGAGGCTCA GGTCTCAGCT CAACATCTAG AAGTACACTT GAAACAGAAA 
GAACAGCACT ACGAGGAAAA GATCAAGGTA TTGGACAATC AGATAAAGAA GGACCTGGCC 
GACAAGGAGA GCCTGGAGAA CATGATGCAG AGACACGAGG AGGAGGCCCA CGAGAAGGGC 
AAGATCCTCA GCGAGCAGAA GGCGATGATC AACGCGATGG ATTCCAAGAT CCGATCCCTG 
GAGCAGAGGA TCGTGGAGCT GTCGGAAGCC AACAAGCTTG CGGCAAACAG CAGTCTCTTC 
ACCCAGAGGA ACATGAAGGC CCAGGAAGAG ATGATCTCAG AACTCAGGCA GCAGAAATTT 
TACCTGGAGA CGCAGGCCGG AAAGCTGGAG GCCCAGAACC GAAAGCTGGA AGAGCAACTG 
GAGAAGATCA GCCACCAAGA TCACAGTGAC AAGAGTCGGC TGCTGGAGCT GGAAACAAGG 
CTGAGGGAGG TCAGCCTGGA GCACGAGGAG CAGAAGCTGG AGCTGAAGCG GCAGCTCACG 
GAGCTGCAGC TGTCCCTGCA GGAGCGCGAG TCCCAGCTGA CGGCCCTGCA GGCCGCCCGG 
GCAGCGCTGG AGAGCCAGCT CCGCCAGGCG AAGACAGAGC TGGAGGAGAC AACCGCGGAA 
GCGGAGGAGG AGATCCAGGC GCTCACGGCA CATCGAGATG AGATCCAGCG CAAATTCGAT 
GCCCTTCGCA ACAGCTGCAC GGTCATCACC GACCTGGAGG AGCAGCTGAA CCAGCTCACC 
GAGGACAACG CCGAGCTCAA CAACCAAAAC TTCTACCTGT CCAAACAACT CGATGAGGCT 
TCCGGGGCCA ATGACGAGAT TGTGCAGCTG CGGAGTGAGG TGGACCATCT CCGCCGTGAG 
ATCACGGAGC GGGAGATGCA GCTCACCAGC CAGAAGCAAG CACAACTCAG TGCTCCTGAT 
TTGCAGACGA TGGAGGCTCT GAAGACGACA TGCACCATGC TGGAGGAGCA GGTCCTGGAC 
CTGGAGGCCC TGAACGACGA GCTGCTGGAG AAGGAGCGCC AGTGGGAGGC CTGGCGGAGC 
GTCCTCGGCG ACGAGAAGTC CCAGTTCGAG TGTCGAGTTC GAGAGCTACA GAGGATGCTG 
GACACCGAGA AGCAGAGCAG GGCTAGGGCC GATCAGCGGA TCACCGAGTC GCGCCAGGTG 
GTGGAGTTGG CGGTGAAGGA ACACAAGGCC GAGATTCTTG CTCTGCAGCA GGCTCTCAAG 
GAGCAGAAGC TCAAAGCCGA GAGCCTGTCG GACAAGCTCA ACGACCTGGA GAAGAAGCAC 
GCCATGCTGG AGATGAACGC CCGGAGCTTA CAGCAGAAAC TAGAGACAGA GCGGGAGCTC 
AAACAGAGGC TTCTGGAGGA GCAAGCCAAA TTGCAGCAGC AGATGGACCT GCAGAAGAAC 
CACATCTTCA GACTGACGCA AGGGCTGCAG GAGGCGCTGG ACCGGGCGGA TCTGCTGAAG 
ACAGAAAGGA GCGACCTGGA GTACCAGCTG GAAAACATTC AGGTTCTCTA CTCTCACGAG 
AAAGTGAAAA TGGAAGGCAC AATCTCTCAG CAAACCAAAC TCATTGATTT CCTGCAAGCC 
AAAATGGACC AGCCTGCTAA AAAGAAAAAG GTTCCTCTGC AGTACAATGA GCTGAAGCTA 
GCCCTGGAAA AGGAGAAAGC CCGATGCGCG GAGCTGGAGG AGGCCCTTCA GAAGACCCGC 
ATCGAACTCC GCTCTGCGCG GGAGGAAGCT GCCCACCGCA AAGCCACGGA CCACCCGCAC 
CCGTCTACGC CAGCCACTGC GAGGCAGCAG ATCGCCATGT CTGCCATTGT GCGGTCGCCC 
GAGCACCAGC CCAGTGCCAT GAGCCTGCTT GCCCCACCAT CCAGCCGCAG AAAGGAGTCG 
TCAACTCCAG AGGAATTCAG CCGGCGTCTG AAAGAGCGCA TGCACCACAA CATCCCTCAC 
CGGTTTAATG TGGGCCTGAA CATGAGAGCC ACCAAGTGCG CCGTGTGTCT GGATACTGTG 
CACTTTGGAC GCCAGGCATC CAAATGCCTC GAATGTCAAG TGATGTGTCA CCCCAAATGC 
TCCACCTGCT TGCCCGCTAC CTGTGGCCTG CCAGCTGAAT ATGCCACACA CTTCACTGAG 
GCCTTCTGCC GGGACAAAAT GAACTCCCCG GGGCTCCAGA GCAAGGAGCC TGGCAGCAGC 
TTGCACCTGG AAGGGTGGAT GAAAGTGCCC AGAAATAACA AACGGGGACA GCAAGGCTGG 
GACAGGAAGT ACATTGTTCT GGAGGGGTCA AAAGTCCTCA TCTATGACAA TGAAGCCAGA 
GAAGCTGGAC AGAGGCCGGT GGAAGAATTT GAGCTGTGCC TTCCTGACGG GGACGTATCT 
ATTCATGGTG CCGTTGGTGC TTCAGAACTT GCAAATACCG CCAAAGCAGA TGTCCCATAC 
ATCCTGAAGA TGGAGTCTCA CCCACACACC ACCTGCTGGC CTGGGAGAAC CCTCTACTTG 
CTAGCACCCA GCTTCCCCGA CAAGCAGCGC TGGGTCACCG CCTTAGAATC TGTCGTCGCA 
GGTGGGAGAG TTTCTAGGGA AAAGGCCGAA GCCGATGCTA AATTACTTGG AAACTCTCTG 
CTGAAACTGG AGGGCGATGA CCGGCTTGAC ATGAACTGCA CCCTGCCCTT CAGTGACCAG 
GTAGTGCTGG TGGGCACGGA GGAAGGCCTC TACGCCCTGA ATGTCTTGAA AAACTCCTTA 
ACCCACATCC CAGGGATTGG CGCAGTCTTC CAAATTTACA TCATCAAGGA CCTGGAGAAG 
CTGCTCATGA TAGCAGGGGA AGAGCGGGCT CTGTGCCTGG TGGACGTGAA GAAGGTGAAG 
CAGTCCCTGG CGCAGTCACA CCTGCCTGCC CAGCCCGACG TCTCCCCCAA CATATTCGAA 
GCCGTCAAAG GCTGCCACTT GTTCGCTGCT GGCAAGATCG AGAACAGCCT GTGCATCTGC 
GCCGCTATGC CAAGCAAAGT CGTCATCCTC CGCTACAATG ACAACCTCAG CAAGTACTGC 
ATCCGCAAGG AGATCGAGAC CTCAGAGCCC TGCAGCTGTA TCCACTTCAC CAACTACAGC 
ATCCTCATTG GGACCAACAA ATTCTATGAG ATCGACATGA AGCAGTACAC GCTTGACGAG 
TTCCTGGACA AGAACGACCA TTCCTTGGCA CCTGCTGTGT TCGCCTCCTC GTCCAACAGC 
TTCCCTGTCT CCATTGTGCA GGCGAACAGC GCCGGGCAGC GAGAAGAATA CCTGCTGTGC 
TTCCACGAAT TTGGGGTGTT CGTGGATTCT TACGGAAGAC GTAGCCGCAC AGATGATCTC 
AAGTGGAGTC GCTTACCTCT GGCCTTCGCC TACAGAGAAC CTTATCTGTT TGTGACTCAC 
TTCAACTCCC TGGAAGTCAT TGAGATCCAG GCCAGATCCT CACTGGGGAG CCCTGCCCGA 
GCATATCTGG AAATTCCAAA CCCTCGCTAC CTGGGCCCCG CGATTTCCTC CGGAGCGATT 
TACCTGGCCT CCTCATACCA GGACAAGTTA AGGGTCATAT GCTGCAAAGG AAACCTCGTG 
AAGGAGTCAG GCACTGAGCA GCACCGGGTG CCCTCCACCT CCCGCAGCAG CCCCAACAAG 
CGAGGCCCAC CAACATACAA CGAGCACATC ACCAAACGCG TGGCCTCCAG CCCGGCGCCA 
CCGGAAGGCC CCAGCCACCC CCGAGAGCCA AGCACACCGC ACCGCTACCG AGACAGAGAG 
GGTCGGACAG AGCTGCGCAG GGACAAGTCT CCAGGCCGCC CTCTGGAGCG GGAGAAGTCC 
CCAGGCCGAA TGCTCAGCAC TAGGAGAGAG CGGTCCCCAG GGAGACTGTT TGAAGACAGC 
AGCAGGGGCC GGCTGCCTGC AGGAGCAGTG AGGACCCCAC TGTCCCAGGT TAACAAGGTC 
TGGGACCAGT CTTCAGTATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cit ( XM_006530146.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006530209.1 Mus musculus citron (Cit), transcript variant X21, mRNA.

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CloneID	OMu35786
Clone ID Related Accession (Same CDS sequence)	XM_006530146.3
Accession Version	XM_006530146.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5385bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	citron Rho-interacting kinase isoform X17
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000071.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006530146.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTGAAGT TCAAGTATGG TGTGCGGAAC CCGCCGGAGG CCAGTGCCTC CGAGCCCATT 
GCCAGTCGGG CCTCCAGGCT AAATCTCTTC TTCCAGGGGA AACCGCCCCT CATGACTCAA 
CAGCAGATGT CTGCTCTTTC CCGGGAAGGG ATGCTAGACG CCCTCTTCGC TCTCTTTGAA 
GAGTGCAGCC AACCCGCCCT GATGAAGATG AAGCACGTGA GCAGCTTTGT CCAGAAGTAT 
TCCGACACCA TAGCCGAGTT GCGGGAGCTG CAGCCGTCGG CGAGAGACTT CGAAGTTCGA 
AGCCTTGTGG GCTGTGGTCA CTTCGCTGAA GTGCAGGTGG TTAGAGAGAA GGCGACCGGG 
GACGTCTATG CCATGAAAAT CATGAAGAAG AAGGCTTTGC TGGCCCAGGA ACAGGTTTCA 
TTTTTCGAGG AGGAGAGGAA CATATTATCT CGGAGCACGA GTCCTTGGAT CCCCCAGTTA 
CAGTACGCCT TTCAGGACAA AAATAACCTT TACCTGGTCA TGGAATATCA GCCTGGAGGG 
GATTTGCTGT CGCTTCTGAA CAGATACGAG GACCAATTAG ATGAGAGCAT GATCCAGTTT 
TACCTTGCTG AGCTGATTTT GGCTGTCCAC AGCGTGCACC AGATGGGATA TGTGCATCGA 
GACATCAAGC CCGAGAACAT CCTCATCGAC CGGACGGGAC ACATCAAGCT GGTGGATTTT 
GGATCAGCCG CTAAGATGAA TTCAAATAAG GTGGATGCCA AACTCCCCAT TGGGACCCCG 
GATTACATGG CTCCGGAAGT GTTGACCGTG ATGAACGAGG ACCGAAGGGG CACATACGGC 
TTGGACTGTG ACTGGTGGTC TGTCGGAGTT GTTGCTTATG AGATGGTTTA TGGGAAGACC 
CCATTCACAG AGGGAACCTC CGCCCGGACC TTCAACAACA TCATGAACTT CCAGCGGTTT 
TTGAAGTTCC CAGATGACCC CAAAGTTAGC AGTGAGCTCC TTGATCTGCT TCAGAGTCTG 
CTGTGTGTCC AGAAAGAGAG ACTGAAGTTC GAGGGTCTCT GCTGCCACCC TTTCTTTGCC 
AGAACGGACT GGAACAACAT CCGTAACTCT CCTCCCCCCT TCGTCCCCAC CCTCAAGTCT 
GACGATGACA CCTCCAATTT TGATGAACCA GAGAAGAATT CGTGGGTTTC ATCCTCTGTG 
TGCCAGCTGA GCCCCTCGGG CTTCTCAGGC GAAGAGCTGC CGTTTGTGGG ATTTTCGTAC 
AGCAAGGCAC TGGGGTATCT TGGTAGATCT GAGTCTGTCG TGTCGAGTCT GGACTCCCCT 
GCCAAGGTTA GCTCCATGGA AAAGAAACTT CTCATCAAAA GCAAAGAGCT CCAAGACTCC 
CAGGACAAGT GTCACAAGAT GGAGCAGGAA ATGACCCGGT TACATCGCAG AGTGTCAGAG 
GTGGAGGCTG TGCTTAGTCA GAAGGAGGTG GAGCTGAAGG CCTCTGAGAC TCAGAGATCC 
CTCCTGGAGC AGGACCTTGC TACCTACATC ACAGAATGCA GTAGCTTAAA GCGAAGTTTG 
GAGCAAGCGC GGATGGAGGT GTCCCAGGAG GATGACAAAG CTCTGCAGCT TCTCCACGAC 
ATCCGAGAGC AGAGCCGGAA GCTCCAGGAG ATCAAGGAGC AGGAGTACCA GGCTCAGGTG 
GAGGAGATGA GGCTGATGAT GAATCAGCTG GAAGAAGACC TGGTGTCAGC CCGCAGACGC 
AGCGATCTCT ACGAGTCTGA GCTGAGGGAG TCTCGGCTTG CCGCCGAGGA ATTCAAGCGG 
AAGGCAAACG AATGTCAGCA CAAACTGATG AAGGTAGTCA GCCACCCTCC AAGAGGGGAC 
TCCGGGGGCA CGGCCCTGGA TGATCTTCAC AAGATGCAAG GCCACGCTGG CTTAACGAGT 
GCTAAGGACC AAGGGAAGCC TGAAGTGGGA GAATATTCCA AACTGGAGAA GATCAATGCT 
GAGCAGCAGC TGAAGATCCA GGAGCTCCAG GAGAAGCTGG AGAAGGCTGT AAAAGCCAGC 
ACAGAGGCCA CCGAGCTCCT GCAGAATATC CGCCAGGCAA AGGAGCGAGC AGAGCGGGAG 
CTAGAGAAGC TACACAACCG GGAAGACTCC TCCGAGGGCA TCAAAAAGAA GCTGGTGGAA 
GCCGAGGAAC GCCGCCACTC CCTGGAGAAC AAGGTAAAGA GACTAGAGAC CATGGAGCGT 
AGAGAGAACA GACTGAAGGA TGACATCCAG ACAAAGTCCG AACAGATCCA GCAGATGGCT 
GATAAAATTC TGGAGCTGGA GGAGAAACAT CGGGAGGCTC AGGTCTCAGC TCAACATCTA 
GAAGTACACT TGAAACAGAA AGAACAGCAC TACGAGGAAA AGATCAAGGT ATTGGACAAT 
CAGATAAAGA AGGACCTGGC CGACAAGGAG AGCCTGGAGA ACATGATGCA GAGACACGAG 
GAGGAGGCCC ACGAGAAGGG CAAGATCCTC AGCGAGCAGA AGGCGATGAT CAACGCGATG 
GATTCCAAGA TCCGATCCCT GGAGCAGAGG ATCGTGGAGC TGTCGGAAGC CAACAAGCTT 
GCGGCAAACA GCAGTCTCTT CACCCAGAGG AACATGAAGG CCCAGGAAGA GATGATCTCA 
GAACTCAGGC AGCAGAAATT TTACCTGGAG ACGCAGGCCG GAAAGCTGGA GGCCCAGAAC 
CGAAAGCTGG AAGAGCAACT GGAGAAGATC AGCCACCAAG ATCACAGTGA CAAGAGTCGG 
CTGCTGGAGC TGGAAACAAG GCTGAGGGAG GTCAGCCTGG AGCACGAGGA GCAGAAGCTG 
GAGCTGAAGC GGCAGCTCAC GGAGCTGCAG CTGTCCCTGC AGGAGCGCGA GTCCCAGCTG 
ACGGCCCTGC AGGCCGCCCG GGCAGCGCTG GAGAGCCAGC TCCGCCAGGC GAAGACAGAG 
CTGGAGGAGA CAACCGCGGA AGCGGAGGAG GAGATCCAGG CGCTCACGGC ACATCGAGAT 
GAGATCCAGC GCAAATTCGA TGCCCTTCGC AACAGCTGCA CGGTCATCAC CGACCTGGAG 
GAGCAGCTGA ACCAGCTCAC CGAGGACAAC GCCGAGCTCA ACAACCAAAA CTTCTACCTG 
TCCAAACAAC TCGATGAGGC TTCCGGGGCC AATGACGAGA TTGTGCAGCT GCGGAGTGAG 
GTGGACCATC TCCGCCGTGA GATCACGGAG CGGGAGATGC AGCTCACCAG CCAGAAGCAA 
GCACAACTCA GTGCTCCTGA TTTGCAGACG ATGGAGGCTC TGAAGACGAC ATGCACCATG 
CTGGAGGAGC AGGTCCTGGA CCTGGAGGCC CTGAACGACG AGCTGCTGGA GAAGGAGCGC 
CAGTGGGAGG CCTGGCGGAG CGTCCTCGGC GACGAGAAGT CCCAGTTCGA GTGTCGAGTT 
CGAGAGCTAC AGAGGATGCT GGACACCGAG AAGCAGAGCA GGGCTAGGGC CGATCAGCGG 
ATCACCGAGT CGCGCCAGGT GGTGGAGTTG GCGGTGAAGG AACACAAGGC CGAGATTCTT 
GCTCTGCAGC AGGCTCTCAA GGAGCAGAAG CTCAAAGCCG AGAGCCTGTC GGACAAGCTC 
AACGACCTGG AGAAGAAGCA CGCCATGCTG GAGATGAACG CCCGGAGCTT ACAGCAGAAA 
CTAGAGACAG AGCGGGAGCT CAAACAGAGG CTTCTGGAGG AGCAAGCCAA ATTGCAGCAG 
CAGATGGACC TGCAGAAGAA CCACATCTTC AGACTGACGC AAGGGCTGCA GGAGGCGCTG 
GACCGGGCGG ATCTGCTGAA GACAGAAAGG AGCGACCTGG AGTACCAGCT GGAAAACATT 
CAGGTTCTCT ACTCTCACGA GAAAGTGAAA ATGGAAGGCA CAATCTCTCA GCAAACCAAA 
CTCATTGATT TCCTGCAAGC CAAAATGGAC CAGCCTGCTA AAAAGAAAAA GGGTTTATTT 
AGTCGACGGA AAGAGGACCC TGCTTTGCCC ACACAGGTTC CTCTGCAGTA CAATGAGCTG 
AAGCTAGCCC TGGAAAAGGA GAAAGCCCGA TGCGCGGAGC TGGAGGAGGC CCTTCAGAAG 
ACCCGCATCG AACTCCGCTC TGCGCGGGAG GAAGCTGCCC ACCGCAAAGC CACGGACCAC 
CCGCACCCGT CTACGCCAGC CACTGCGAGG CAGCAGATCG CCATGTCTGC CATTGTGCGG 
TCGCCCGAGC ACCAGCCCAG TGCCATGAGC CTGCTTGCCC CACCATCCAG CCGCAGAAAG 
GAGTCGTCAA CTCCAGAGGA ATTCAGCCGG CGTCTGAAAG AGCGCATGCA CCACAACATC 
CCTCACCGGT TTAATGTGGG CCTGAACATG AGAGCCACCA AGTGCGCCGT GTGTCTGGAT 
ACTGTGCACT TTGGACGCCA GGCATCCAAA TGCCTCGAAT GTCAAGTGAT GTGTCACCCC 
AAATGCTCCA CCTGCTTGCC CGCTACCTGT GGCCTGCCAG CTGAATATGC CACACACTTC 
ACTGAGGCCT TCTGCCGGGA CAAAATGAAC TCCCCGGGGC TCCAGAGCAA GGAGCCTGGC 
AGCAGCTTGC ACCTGGAAGG GTGGATGAAA GTGCCCAGAA ATAACAAACG GGGACAGCAA 
GGCTGGGACA GGAAGTACAT TGTTCTGGAG GGGTCAAAAG TCCTCATCTA TGACAATGAA 
GCCAGAGAAG CTGGACAGAG GCCGGTGGAA GAATTTGAGC TGTGCCTTCC TGACGGGGAC 
GTATCTATTC ATGGTGCCGT TGGTGCTTCA GAACTTGCAA ATACCGCCAA AGCAGATGTC 
CCATACATCC TGAAGATGGA GTCTCACCCA CACACCACCT GCTGGCCTGG GAGAACCCTC 
TACTTGCTAG CACCCAGCTT CCCCGACAAG CAGCGCTGGG TCACCGCCTT AGAATCTGTC 
GTCGCAGGTG GGAGAGTTTC TAGGGAAAAG GCCGAAGCCG ATGCTGCACG TGACCATACT 
TCGAGTGAGC ATCAGCCAGT GTGGGTTGAG AAATTACTTG GAAACTCTCT GCTGAAACTG 
GAGGGCGATG ACCGGCTTGA CATGAACTGC ACCCTGCCCT TCAGTGACCA GGTAGTGCTG 
GTGGGCACGG AGGAAGGCCT CTACGCCCTG AATGTCTTGA AAAACTCCTT AACCCACATC 
CCAGGGATTG GCGCAGTCTT CCAAATTTAC ATCATCAAGG ACCTGGAGAA GCTGCTCATG 
ATAGCAGGAA TACTGCGACT ATCCCGTCAG GCCCTGTGTG GGCTGGGACC TCCCGAGAGC 
TCGCCTCCTG CCCTGGAGGC ACCTGTGATG TGGTTACTAC AGAATCCTTC CTGTGTTCAT 
GTGTGCCCAG ACCCCTCCTC TGGATTCATA CTTTCCAACA TTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006530209.1
CDS	160..5544
Translation	MLKFKYGVRNPPEASASEPIASRASRLNLFFQGKPPLMTQQQMSALSREGMLDALFALFEECSQPALMKMKHVSSFVQKYSDTIAELRELQPSARDFEVRSLVGCGHFAEVQVVREKATGDVYAMKIMKKKALLAQEQVSFFEEERNILSRSTSPWIPQLQYAFQDKNNLYLVMEYQPGGDLLSLLNRYEDQLDESMIQFYLAELILAVHSVHQMGYVHRDIKPENILIDRTGHIKLVDFGSAAKMNSNKVDAKLPIGTPDYMAPEVLTVMNEDRRGTYGLDCDWWSVGVVAYEMVYGKTPFTEGTSARTFNNIMNFQRFLKFPDDPKVSSELLDLLQSLLCVQKERLKFEGLCCHPFFARTDWNNIRNSPPPFVPTLKSDDDTSNFDEPEKNSWVSSSVCQLSPSGFSGEELPFVGFSYSKALGYLGRSESVVSSLDSPAKVSSMEKKLLIKSKELQDSQDKCHKMEQEMTRLHRRVSEVEAVLSQKEVELKASETQRSLLEQDLATYITECSSLKRSLEQARMEVSQEDDKALQLLHDIREQSRKLQEIKEQEYQAQVEEMRLMMNQLEEDLVSARRRSDLYESELRESRLAAEEFKRKANECQHKLMKVVSHPPRGDSGGTALDDLHKMQGHAGLTSAKDQGKPEVGEYSKLEKINAEQQLKIQELQEKLEKAVKASTEATELLQNIRQAKERAERELEKLHNREDSSEGIKKKLVEAEERRHSLENKVKRLETMERRENRLKDDIQTKSEQIQQMADKILELEEKHREAQVSAQHLEVHLKQKEQHYEEKIKVLDNQIKKDLADKESLENMMQRHEEEAHEKGKILSEQKAMINAMDSKIRSLEQRIVELSEANKLAANSSLFTQRNMKAQEEMISELRQQKFYLETQAGKLEAQNRKLEEQLEKISHQDHSDKSRLLELETRLREVSLEHEEQKLELKRQLTELQLSLQERESQLTALQAARAALESQLRQAKTELEETTAEAEEEIQALTAHRDEIQRKFDALRNSCTVITDLEEQLNQLTEDNAELNNQNFYLSKQLDEASGANDEIVQLRSEVDHLRREITEREMQLTSQKQAQLSAPDLQTMEALKTTCTMLEEQVLDLEALNDELLEKERQWEAWRSVLGDEKSQFECRVRELQRMLDTEKQSRARADQRITESRQVVELAVKEHKAEILALQQALKEQKLKAESLSDKLNDLEKKHAMLEMNARSLQQKLETERELKQRLLEEQAKLQQQMDLQKNHIFRLTQGLQEALDRADLLKTERSDLEYQLENIQVLYSHEKVKMEGTISQQTKLIDFLQAKMDQPAKKKKGLFSRRKEDPALPTQVPLQYNELKLALEKEKARCAELEEALQKTRIELRSAREEAAHRKATDHPHPSTPATARQQIAMSAIVRSPEHQPSAMSLLAPPSSRRKESSTPEEFSRRLKERMHHNIPHRFNVGLNMRATKCAVCLDTVHFGRQASKCLECQVMCHPKCSTCLPATCGLPAEYATHFTEAFCRDKMNSPGLQSKEPGSSLHLEGWMKVPRNNKRGQQGWDRKYIVLEGSKVLIYDNEAREAGQRPVEEFELCLPDGDVSIHGAVGASELANTAKADVPYILKMESHPHTTCWPGRTLYLLAPSFPDKQRWVTALESVVAGGRVSREKAEADAARDHTSSEHQPVWVEKLLGNSLLKLEGDDRLDMNCTLPFSDQVVLVGTEEGLYALNVLKNSLTHIPGIGAVFQIYIIKDLEKLLMIAGILRLSRQALCGLGPPESSPPALEAPVMWLLQNPSCVHVCPDPSSGFILSNI

Target ORF information:

RefSeq Version	XM_006530146.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus citron (Cit), transcript variant X21, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006530146.3

ORF Insert Sequence:

ATGTTGAAGT TCAAGTATGG TGTGCGGAAC CCGCCGGAGG CCAGTGCCTC CGAGCCCATT 
GCCAGTCGGG CCTCCAGGCT AAATCTCTTC TTCCAGGGGA AACCGCCCCT CATGACTCAA 
CAGCAGATGT CTGCTCTTTC CCGGGAAGGG ATGCTAGACG CCCTCTTCGC TCTCTTTGAA 
GAGTGCAGCC AACCCGCCCT GATGAAGATG AAGCACGTGA GCAGCTTTGT CCAGAAGTAT 
TCCGACACCA TAGCCGAGTT GCGGGAGCTG CAGCCGTCGG CGAGAGACTT CGAAGTTCGA 
AGCCTTGTGG GCTGTGGTCA CTTCGCTGAA GTGCAGGTGG TTAGAGAGAA GGCGACCGGG 
GACGTCTATG CCATGAAAAT CATGAAGAAG AAGGCTTTGC TGGCCCAGGA ACAGGTTTCA 
TTTTTCGAGG AGGAGAGGAA CATATTATCT CGGAGCACGA GTCCTTGGAT CCCCCAGTTA 
CAGTACGCCT TTCAGGACAA AAATAACCTT TACCTGGTCA TGGAATATCA GCCTGGAGGG 
GATTTGCTGT CGCTTCTGAA CAGATACGAG GACCAATTAG ATGAGAGCAT GATCCAGTTT 
TACCTTGCTG AGCTGATTTT GGCTGTCCAC AGCGTGCACC AGATGGGATA TGTGCATCGA 
GACATCAAGC CCGAGAACAT CCTCATCGAC CGGACGGGAC ACATCAAGCT GGTGGATTTT 
GGATCAGCCG CTAAGATGAA TTCAAATAAG GTGGATGCCA AACTCCCCAT TGGGACCCCG 
GATTACATGG CTCCGGAAGT GTTGACCGTG ATGAACGAGG ACCGAAGGGG CACATACGGC 
TTGGACTGTG ACTGGTGGTC TGTCGGAGTT GTTGCTTATG AGATGGTTTA TGGGAAGACC 
CCATTCACAG AGGGAACCTC CGCCCGGACC TTCAACAACA TCATGAACTT CCAGCGGTTT 
TTGAAGTTCC CAGATGACCC CAAAGTTAGC AGTGAGCTCC TTGATCTGCT TCAGAGTCTG 
CTGTGTGTCC AGAAAGAGAG ACTGAAGTTC GAGGGTCTCT GCTGCCACCC TTTCTTTGCC 
AGAACGGACT GGAACAACAT CCGTAACTCT CCTCCCCCCT TCGTCCCCAC CCTCAAGTCT 
GACGATGACA CCTCCAATTT TGATGAACCA GAGAAGAATT CGTGGGTTTC ATCCTCTGTG 
TGCCAGCTGA GCCCCTCGGG CTTCTCAGGC GAAGAGCTGC CGTTTGTGGG ATTTTCGTAC 
AGCAAGGCAC TGGGGTATCT TGGTAGATCT GAGTCTGTCG TGTCGAGTCT GGACTCCCCT 
GCCAAGGTTA GCTCCATGGA AAAGAAACTT CTCATCAAAA GCAAAGAGCT CCAAGACTCC 
CAGGACAAGT GTCACAAGAT GGAGCAGGAA ATGACCCGGT TACATCGCAG AGTGTCAGAG 
GTGGAGGCTG TGCTTAGTCA GAAGGAGGTG GAGCTGAAGG CCTCTGAGAC TCAGAGATCC 
CTCCTGGAGC AGGACCTTGC TACCTACATC ACAGAATGCA GTAGCTTAAA GCGAAGTTTG 
GAGCAAGCGC GGATGGAGGT GTCCCAGGAG GATGACAAAG CTCTGCAGCT TCTCCACGAC 
ATCCGAGAGC AGAGCCGGAA GCTCCAGGAG ATCAAGGAGC AGGAGTACCA GGCTCAGGTG 
GAGGAGATGA GGCTGATGAT GAATCAGCTG GAAGAAGACC TGGTGTCAGC CCGCAGACGC 
AGCGATCTCT ACGAGTCTGA GCTGAGGGAG TCTCGGCTTG CCGCCGAGGA ATTCAAGCGG 
AAGGCAAACG AATGTCAGCA CAAACTGATG AAGGTAGTCA GCCACCCTCC AAGAGGGGAC 
TCCGGGGGCA CGGCCCTGGA TGATCTTCAC AAGATGCAAG GCCACGCTGG CTTAACGAGT 
GCTAAGGACC AAGGGAAGCC TGAAGTGGGA GAATATTCCA AACTGGAGAA GATCAATGCT 
GAGCAGCAGC TGAAGATCCA GGAGCTCCAG GAGAAGCTGG AGAAGGCTGT AAAAGCCAGC 
ACAGAGGCCA CCGAGCTCCT GCAGAATATC CGCCAGGCAA AGGAGCGAGC AGAGCGGGAG 
CTAGAGAAGC TACACAACCG GGAAGACTCC TCCGAGGGCA TCAAAAAGAA GCTGGTGGAA 
GCCGAGGAAC GCCGCCACTC CCTGGAGAAC AAGGTAAAGA GACTAGAGAC CATGGAGCGT 
AGAGAGAACA GACTGAAGGA TGACATCCAG ACAAAGTCCG AACAGATCCA GCAGATGGCT 
GATAAAATTC TGGAGCTGGA GGAGAAACAT CGGGAGGCTC AGGTCTCAGC TCAACATCTA 
GAAGTACACT TGAAACAGAA AGAACAGCAC TACGAGGAAA AGATCAAGGT ATTGGACAAT 
CAGATAAAGA AGGACCTGGC CGACAAGGAG AGCCTGGAGA ACATGATGCA GAGACACGAG 
GAGGAGGCCC ACGAGAAGGG CAAGATCCTC AGCGAGCAGA AGGCGATGAT CAACGCGATG 
GATTCCAAGA TCCGATCCCT GGAGCAGAGG ATCGTGGAGC TGTCGGAAGC CAACAAGCTT 
GCGGCAAACA GCAGTCTCTT CACCCAGAGG AACATGAAGG CCCAGGAAGA GATGATCTCA 
GAACTCAGGC AGCAGAAATT TTACCTGGAG ACGCAGGCCG GAAAGCTGGA GGCCCAGAAC 
CGAAAGCTGG AAGAGCAACT GGAGAAGATC AGCCACCAAG ATCACAGTGA CAAGAGTCGG 
CTGCTGGAGC TGGAAACAAG GCTGAGGGAG GTCAGCCTGG AGCACGAGGA GCAGAAGCTG 
GAGCTGAAGC GGCAGCTCAC GGAGCTGCAG CTGTCCCTGC AGGAGCGCGA GTCCCAGCTG 
ACGGCCCTGC AGGCCGCCCG GGCAGCGCTG GAGAGCCAGC TCCGCCAGGC GAAGACAGAG 
CTGGAGGAGA CAACCGCGGA AGCGGAGGAG GAGATCCAGG CGCTCACGGC ACATCGAGAT 
GAGATCCAGC GCAAATTCGA TGCCCTTCGC AACAGCTGCA CGGTCATCAC CGACCTGGAG 
GAGCAGCTGA ACCAGCTCAC CGAGGACAAC GCCGAGCTCA ACAACCAAAA CTTCTACCTG 
TCCAAACAAC TCGATGAGGC TTCCGGGGCC AATGACGAGA TTGTGCAGCT GCGGAGTGAG 
GTGGACCATC TCCGCCGTGA GATCACGGAG CGGGAGATGC AGCTCACCAG CCAGAAGCAA 
GCACAACTCA GTGCTCCTGA TTTGCAGACG ATGGAGGCTC TGAAGACGAC ATGCACCATG 
CTGGAGGAGC AGGTCCTGGA CCTGGAGGCC CTGAACGACG AGCTGCTGGA GAAGGAGCGC 
CAGTGGGAGG CCTGGCGGAG CGTCCTCGGC GACGAGAAGT CCCAGTTCGA GTGTCGAGTT 
CGAGAGCTAC AGAGGATGCT GGACACCGAG AAGCAGAGCA GGGCTAGGGC CGATCAGCGG 
ATCACCGAGT CGCGCCAGGT GGTGGAGTTG GCGGTGAAGG AACACAAGGC CGAGATTCTT 
GCTCTGCAGC AGGCTCTCAA GGAGCAGAAG CTCAAAGCCG AGAGCCTGTC GGACAAGCTC 
AACGACCTGG AGAAGAAGCA CGCCATGCTG GAGATGAACG CCCGGAGCTT ACAGCAGAAA 
CTAGAGACAG AGCGGGAGCT CAAACAGAGG CTTCTGGAGG AGCAAGCCAA ATTGCAGCAG 
CAGATGGACC TGCAGAAGAA CCACATCTTC AGACTGACGC AAGGGCTGCA GGAGGCGCTG 
GACCGGGCGG ATCTGCTGAA GACAGAAAGG AGCGACCTGG AGTACCAGCT GGAAAACATT 
CAGGTTCTCT ACTCTCACGA GAAAGTGAAA ATGGAAGGCA CAATCTCTCA GCAAACCAAA 
CTCATTGATT TCCTGCAAGC CAAAATGGAC CAGCCTGCTA AAAAGAAAAA GGGTTTATTT 
AGTCGACGGA AAGAGGACCC TGCTTTGCCC ACACAGGTTC CTCTGCAGTA CAATGAGCTG 
AAGCTAGCCC TGGAAAAGGA GAAAGCCCGA TGCGCGGAGC TGGAGGAGGC CCTTCAGAAG 
ACCCGCATCG AACTCCGCTC TGCGCGGGAG GAAGCTGCCC ACCGCAAAGC CACGGACCAC 
CCGCACCCGT CTACGCCAGC CACTGCGAGG CAGCAGATCG CCATGTCTGC CATTGTGCGG 
TCGCCCGAGC ACCAGCCCAG TGCCATGAGC CTGCTTGCCC CACCATCCAG CCGCAGAAAG 
GAGTCGTCAA CTCCAGAGGA ATTCAGCCGG CGTCTGAAAG AGCGCATGCA CCACAACATC 
CCTCACCGGT TTAATGTGGG CCTGAACATG AGAGCCACCA AGTGCGCCGT GTGTCTGGAT 
ACTGTGCACT TTGGACGCCA GGCATCCAAA TGCCTCGAAT GTCAAGTGAT GTGTCACCCC 
AAATGCTCCA CCTGCTTGCC CGCTACCTGT GGCCTGCCAG CTGAATATGC CACACACTTC 
ACTGAGGCCT TCTGCCGGGA CAAAATGAAC TCCCCGGGGC TCCAGAGCAA GGAGCCTGGC 
AGCAGCTTGC ACCTGGAAGG GTGGATGAAA GTGCCCAGAA ATAACAAACG GGGACAGCAA 
GGCTGGGACA GGAAGTACAT TGTTCTGGAG GGGTCAAAAG TCCTCATCTA TGACAATGAA 
GCCAGAGAAG CTGGACAGAG GCCGGTGGAA GAATTTGAGC TGTGCCTTCC TGACGGGGAC 
GTATCTATTC ATGGTGCCGT TGGTGCTTCA GAACTTGCAA ATACCGCCAA AGCAGATGTC 
CCATACATCC TGAAGATGGA GTCTCACCCA CACACCACCT GCTGGCCTGG GAGAACCCTC 
TACTTGCTAG CACCCAGCTT CCCCGACAAG CAGCGCTGGG TCACCGCCTT AGAATCTGTC 
GTCGCAGGTG GGAGAGTTTC TAGGGAAAAG GCCGAAGCCG ATGCTGCACG TGACCATACT 
TCGAGTGAGC ATCAGCCAGT GTGGGTTGAG AAATTACTTG GAAACTCTCT GCTGAAACTG 
GAGGGCGATG ACCGGCTTGA CATGAACTGC ACCCTGCCCT TCAGTGACCA GGTAGTGCTG 
GTGGGCACGG AGGAAGGCCT CTACGCCCTG AATGTCTTGA AAAACTCCTT AACCCACATC 
CCAGGGATTG GCGCAGTCTT CCAAATTTAC ATCATCAAGG ACCTGGAGAA GCTGCTCATG 
ATAGCAGGAA TACTGCGACT ATCCCGTCAG GCCCTGTGTG GGCTGGGACC TCCCGAGAGC 
TCGCCTCCTG CCCTGGAGGC ACCTGTGATG TGGTTACTAC AGAATCCTTC CTGTGTTCAT 
GTGTGCCCAG ACCCCTCCTC TGGATTCATA CTTTCCAACA TTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cit ( XM_006530148.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006530211.1 Mus musculus citron (Cit), transcript variant X20, mRNA.

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CloneID	OMu35787
Clone ID Related Accession (Same CDS sequence)	XM_006530148.3 , XM_006530147.2 , XM_006530148.4 , XM_006530147.1
Accession Version	XM_006530148.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5037bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	citron Rho-interacting kinase isoform X16
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000071.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006530148.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAAAGA AACTTCTCAT CAAAAGCAAA GAGCTCCAAG ACTCCCAGGA CAAGTGTCAC 
AAGATGGAGC AGGAAATGAC CCGGTTACAT CGCAGAGTGT CAGAGGTGGA GGCTGTGCTT 
AGTCAGAAGG AGGTGGAGCT GAAGGCCTCT GAGACTCAGA GATCCCTCCT GGAGCAGGAC 
CTTGCTACCT ACATCACAGA ATGCAGTAGC TTAAAGCGAA GTTTGGAGCA AGCGCGGATG 
GAGGTGTCCC AGGAGGATGA CAAAGCTCTG CAGCTTCTCC ACGACATCCG AGAGCAGAGC 
CGGAAGCTCC AGGAGATCAA GGAGCAGGAG TACCAGGCTC AGGTGGAGGA GATGAGGCTG 
ATGATGAATC AGCTGGAAGA AGACCTGGTG TCAGCCCGCA GACGCAGCGA TCTCTACGAG 
TCTGAGCTGA GGGAGTCTCG GCTTGCCGCC GAGGAATTCA AGCGGAAGGC AAACGAATGT 
CAGCACAAAC TGATGAAGGT AGTCAGCCAC CCTCCAAGAG GGGACTCCGG GGGCACGGCC 
CTGGATGATC TTCACAAGAT GCAAGGCCAC GCTGGCTTAA CGAGTGCTAA GGACCAAGGG 
AAGCCTGAAG TGGGAGAATA TTCCAAACTG GAGAAGATCA ATGCTGAGCA GCAGCTGAAG 
ATCCAGGAGC TCCAGGAGAA GCTGGAGAAG GCTGTAAAAG CCAGCACAGA GGCCACCGAG 
CTCCTGCAGA ATATCCGCCA GGCAAAGGAG CGAGCAGAGC GGGAGCTAGA GAAGCTACAC 
AACCGGGAAG ACTCCTCCGA GGGCATCAAA AAGAAGCTGG TGGAAGCCGA GGAACGCCGC 
CACTCCCTGG AGAACAAGGT AAAGAGACTA GAGACCATGG AGCGTAGAGA GAACAGACTG 
AAGGATGACA TCCAGACAAA GTCCGAACAG ATCCAGCAGA TGGCTGATAA AATTCTGGAG 
CTGGAGGAGA AACATCGGGA GGCTCAGGTC TCAGCTCAAC ATCTAGAAGT ACACTTGAAA 
CAGAAAGAAC AGCACTACGA GGAAAAGATC AAGGTATTGG ACAATCAGAT AAAGAAGGAC 
CTGGCCGACA AGGAGAGCCT GGAGAACATG ATGCAGAGAC ACGAGGAGGA GGCCCACGAG 
AAGGGCAAGA TCCTCAGCGA GCAGAAGGCG ATGATCAACG CGATGGATTC CAAGATCCGA 
TCCCTGGAGC AGAGGATCGT GGAGCTGTCG GAAGCCAACA AGCTTGCGGC AAACAGCAGT 
CTCTTCACCC AGAGGAACAT GAAGGCCCAG GAAGAGATGA TCTCAGAACT CAGGCAGCAG 
AAATTTTACC TGGAGACGCA GGCCGGAAAG CTGGAGGCCC AGAACCGAAA GCTGGAAGAG 
CAACTGGAGA AGATCAGCCA CCAAGATCAC AGTGACAAGA GTCGGCTGCT GGAGCTGGAA 
ACAAGGCTGA GGGAGGTCAG CCTGGAGCAC GAGGAGCAGA AGCTGGAGCT GAAGCGGCAG 
CTCACGGAGC TGCAGCTGTC CCTGCAGGAG CGCGAGTCCC AGCTGACGGC CCTGCAGGCC 
GCCCGGGCAG CGCTGGAGAG CCAGCTCCGC CAGGCGAAGA CAGAGCTGGA GGAGACAACC 
GCGGAAGCGG AGGAGGAGAT CCAGGCGCTC ACGGCACATC GAGATGAGAT CCAGCGCAAA 
TTCGATGCCC TTCGCAACAG CTGCACGGTC ATCACCGACC TGGAGGAGCA GCTGAACCAG 
CTCACCGAGG ACAACGCCGA GCTCAACAAC CAAAACTTCT ACCTGTCCAA ACAACTCGAT 
GAGGCTTCCG GGGCCAATGA CGAGATTGTG CAGCTGCGGA GTGAGGTGGA CCATCTCCGC 
CGTGAGATCA CGGAGCGGGA GATGCAGCTC ACCAGCCAGA AGCAAGCACA ACTCAGTGCT 
CCTGATTTGC AGACGATGGA GGCTCTGAAG ACGACATGCA CCATGCTGGA GGAGCAGGTC 
CTGGACCTGG AGGCCCTGAA CGACGAGCTG CTGGAGAAGG AGCGCCAGTG GGAGGCCTGG 
CGGAGCGTCC TCGGCGACGA GAAGTCCCAG TTCGAGTGTC GAGTTCGAGA GCTACAGAGG 
ATGCTGGACA CCGAGAAGCA GAGCAGGGCT AGGGCCGATC AGCGGATCAC CGAGTCGCGC 
CAGGTGGTGG AGTTGGCGGT GAAGGAACAC AAGGCCGAGA TTCTTGCTCT GCAGCAGGCT 
CTCAAGGAGC AGAAGCTCAA AGCCGAGAGC CTGTCGGACA AGCTCAACGA CCTGGAGAAG 
AAGCACGCCA TGCTGGAGAT GAACGCCCGG AGCTTACAGC AGAAACTAGA GACAGAGCGG 
GAGCTCAAAC AGAGGCTTCT GGAGGAGCAA GCCAAATTGC AGCAGCAGAT GGACCTGCAG 
AAGAACCACA TCTTCAGACT GACGCAAGGG CTGCAGGAGG CGCTGGACCG GGCGGATCTG 
CTGAAGACAG AAAGGAGCGA CCTGGAGTAC CAGCTGGAAA ACATTCAGGT TCTCTACTCT 
CACGAGAAAG TGAAAATGGA AGGCACAATC TCTCAGCAAA CCAAACTCAT TGATTTCCTG 
CAAGCCAAAA TGGACCAGCC TGCTAAAAAG AAAAAGGGTT TATTTAGTCG ACGGAAAGAG 
GACCCTGCTT TGCCCACACA GGTTCCTCTG CAGTACAATG AGCTGAAGCT AGCCCTGGAA 
AAGGAGAAAG CCCGATGCGC GGAGCTGGAG GAGGCCCTTC AGAAGACCCG CATCGAACTC 
CGCTCTGCGC GGGAGGAAGC TGCCCACCGC AAAGCCACGG ACCACCCGCA CCCGTCTACG 
CCAGCCACTG CGAGGCAGCA GATCGCCATG TCTGCCATTG TGCGGTCGCC CGAGCACCAG 
CCCAGTGCCA TGAGCCTGCT TGCCCCACCA TCCAGCCGCA GAAAGGAGTC GTCAACTCCA 
GAGGAATTCA GCCGGCGTCT GAAAGAGCGC ATGCACCACA ACATCCCTCA CCGGTTTAAT 
GTGGGCCTGA ACATGAGAGC CACCAAGTGC GCCGTGTGTC TGGATACTGT GCACTTTGGA 
CGCCAGGCAT CCAAATGCCT CGAATGTCAA GTGATGTGTC ACCCCAAATG CTCCACCTGC 
TTGCCCGCTA CCTGTGGCCT GCCAGCTGAA TATGCCACAC ACTTCACTGA GGCCTTCTGC 
CGGGACAAAA TGAACTCCCC GGGGCTCCAG AGCAAGGAGC CTGGCAGCAG CTTGCACCTG 
GAAGGGTGGA TGAAAGTGCC CAGAAATAAC AAACGGGGAC AGCAAGGCTG GGACAGGAAG 
TACATTGTTC TGGAGGGGTC AAAAGTCCTC ATCTATGACA ATGAAGCCAG AGAAGCTGGA 
CAGAGGCCGG TGGAAGAATT TGAGCTGTGC CTTCCTGACG GGGACGTATC TATTCATGGT 
GCCGTTGGTG CTTCAGAACT TGCAAATACC GCCAAAGCAG ATGTCCCATA CATCCTGAAG 
ATGGAGTCTC ACCCACACAC CACCTGCTGG CCTGGGAGAA CCCTCTACTT GCTAGCACCC 
AGCTTCCCCG ACAAGCAGCG CTGGGTCACC GCCTTAGAAT CTGTCGTCGC AGGTGGGAGA 
GTTTCTAGGG AAAAGGCCGA AGCCGATGCT GCACGTGACC ATACTTCGAG TGAGCATCAG 
CCAGTGTGGG TTGAGAAATT ACTTGGAAAC TCTCTGCTGA AACTGGAGGG CGATGACCGG 
CTTGACATGA ACTGCACCCT GCCCTTCAGT GACCAGGTAG TGCTGGTGGG CACGGAGGAA 
GGCCTCTACG CCCTGAATGT CTTGAAAAAC TCCTTAACCC ACATCCCAGG GATTGGCGCA 
GTCTTCCAAA TTTACATCAT CAAGGACCTG GAGAAGCTGC TCATGATAGC AGGGGAAGAG 
CGGGCTCTGT GCCTGGTGGA CGTGAAGAAG GTGAAGCAGT CCCTGGCGCA GTCACACCTG 
CCTGCCCAGC CCGACGTCTC CCCCAACATA TTCGAAGCCG TCAAAGGCTG CCACTTGTTC 
GCTGCTGGCA AGATCGAGAA CAGCCTGTGC ATCTGCGCCG CTATGCCAAG CAAAGTCGTC 
ATCCTCCGCT ACAATGACAA CCTCAGCAAG TACTGCATCC GCAAGGAGAT CGAGACCTCA 
GAGCCCTGCA GCTGTATCCA CTTCACCAAC TACAGCATCC TCATTGGGAC CAACAAATTC 
TATGAGATCG ACATGAAGCA GTACACGCTT GACGAGTTCC TGGACAAGAA CGACCATTCC 
TTGGCACCTG CTGTGTTCGC CTCCTCGTCC AACAGCTTCC CTGTCTCCAT TGTGCAGGCG 
AACAGCGCCG GGCAGCGAGA AGAATACCTG CTGTGCTTCC ACGAATTTGG GGTGTTCGTG 
GATTCTTACG GAAGACGTAG CCGCACAGAT GATCTCAAGT GGAGTCGCTT ACCTCTGGCC 
TTCGCCTACA GAGAACCTTA TCTGTTTGTG ACTCACTTCA ACTCCCTGGA AGTCATTGAG 
ATCCAGGCCA GATCCTCACT GGGGAGCCCT GCCCGAGCAT ATCTGGAAAT TCCAAACCCT 
CGCTACCTGG GCCCCGCGAT TTCCTCCGGA GCGATTTACC TGGCCTCCTC ATACCAGGAC 
AAGTTAAGGG TCATATGCTG CAAAGGAAAC CTCGTGAAGG AGTCAGGCAC TGAGCAGCAC 
CGGGTGCCCT CCACCTCCCG CAGCAGCCCC AACAAGCGAG GCCCACCAAC ATACAACGAG 
CACATCACCA AACGCGTGGC CTCCAGCCCG GCGCCACCGG AAGGCCCCAG CCACCCCCGA 
GAGCCAAGCA CACCGCACCG CTACCGAGAC AGAGAGGGTC GGACAGAGCT GCGCAGGGAC 
AAGTCTCCAG GCCGCCCTCT GGAGCGGGAG AAGTCCCCAG GCCGAATGCT CAGCACTAGG 
AGAGAGCGGT CCCCAGGGAG ACTGTTTGAA GACAGCAGCA GGGGCCGGCT GCCTGCAGGA 
GCAGTGAGGA CCCCACTGTC CCAGGTTAAC AAGGTCTGGG ACCAGTCTTC AGTATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006530211.1
CDS	100..5136
Translation	MEKKLLIKSKELQDSQDKCHKMEQEMTRLHRRVSEVEAVLSQKEVELKASETQRSLLEQDLATYITECSSLKRSLEQARMEVSQEDDKALQLLHDIREQSRKLQEIKEQEYQAQVEEMRLMMNQLEEDLVSARRRSDLYESELRESRLAAEEFKRKANECQHKLMKVVSHPPRGDSGGTALDDLHKMQGHAGLTSAKDQGKPEVGEYSKLEKINAEQQLKIQELQEKLEKAVKASTEATELLQNIRQAKERAERELEKLHNREDSSEGIKKKLVEAEERRHSLENKVKRLETMERRENRLKDDIQTKSEQIQQMADKILELEEKHREAQVSAQHLEVHLKQKEQHYEEKIKVLDNQIKKDLADKESLENMMQRHEEEAHEKGKILSEQKAMINAMDSKIRSLEQRIVELSEANKLAANSSLFTQRNMKAQEEMISELRQQKFYLETQAGKLEAQNRKLEEQLEKISHQDHSDKSRLLELETRLREVSLEHEEQKLELKRQLTELQLSLQERESQLTALQAARAALESQLRQAKTELEETTAEAEEEIQALTAHRDEIQRKFDALRNSCTVITDLEEQLNQLTEDNAELNNQNFYLSKQLDEASGANDEIVQLRSEVDHLRREITEREMQLTSQKQAQLSAPDLQTMEALKTTCTMLEEQVLDLEALNDELLEKERQWEAWRSVLGDEKSQFECRVRELQRMLDTEKQSRARADQRITESRQVVELAVKEHKAEILALQQALKEQKLKAESLSDKLNDLEKKHAMLEMNARSLQQKLETERELKQRLLEEQAKLQQQMDLQKNHIFRLTQGLQEALDRADLLKTERSDLEYQLENIQVLYSHEKVKMEGTISQQTKLIDFLQAKMDQPAKKKKGLFSRRKEDPALPTQVPLQYNELKLALEKEKARCAELEEALQKTRIELRSAREEAAHRKATDHPHPSTPATARQQIAMSAIVRSPEHQPSAMSLLAPPSSRRKESSTPEEFSRRLKERMHHNIPHRFNVGLNMRATKCAVCLDTVHFGRQASKCLECQVMCHPKCSTCLPATCGLPAEYATHFTEAFCRDKMNSPGLQSKEPGSSLHLEGWMKVPRNNKRGQQGWDRKYIVLEGSKVLIYDNEAREAGQRPVEEFELCLPDGDVSIHGAVGASELANTAKADVPYILKMESHPHTTCWPGRTLYLLAPSFPDKQRWVTALESVVAGGRVSREKAEADAARDHTSSEHQPVWVEKLLGNSLLKLEGDDRLDMNCTLPFSDQVVLVGTEEGLYALNVLKNSLTHIPGIGAVFQIYIIKDLEKLLMIAGEERALCLVDVKKVKQSLAQSHLPAQPDVSPNIFEAVKGCHLFAAGKIENSLCICAAMPSKVVILRYNDNLSKYCIRKEIETSEPCSCIHFTNYSILIGTNKFYEIDMKQYTLDEFLDKNDHSLAPAVFASSSNSFPVSIVQANSAGQREEYLLCFHEFGVFVDSYGRRSRTDDLKWSRLPLAFAYREPYLFVTHFNSLEVIEIQARSSLGSPARAYLEIPNPRYLGPAISSGAIYLASSYQDKLRVICCKGNLVKESGTEQHRVPSTSRSSPNKRGPPTYNEHITKRVASSPAPPEGPSHPREPSTPHRYRDREGRTELRRDKSPGRPLEREKSPGRMLSTRRERSPGRLFEDSSRGRLPAGAVRTPLSQVNKVWDQSSV

Target ORF information:

RefSeq Version	XM_006530148.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus citron (Cit), transcript variant X20, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006530148.3

ORF Insert Sequence:

ATGGAAAAGA AACTTCTCAT CAAAAGCAAA GAGCTCCAAG ACTCCCAGGA CAAGTGTCAC 
AAGATGGAGC AGGAAATGAC CCGGTTACAT CGCAGAGTGT CAGAGGTGGA GGCTGTGCTT 
AGTCAGAAGG AGGTGGAGCT GAAGGCCTCT GAGACTCAGA GATCCCTCCT GGAGCAGGAC 
CTTGCTACCT ACATCACAGA ATGCAGTAGC TTAAAGCGAA GTTTGGAGCA AGCGCGGATG 
GAGGTGTCCC AGGAGGATGA CAAAGCTCTG CAGCTTCTCC ACGACATCCG AGAGCAGAGC 
CGGAAGCTCC AGGAGATCAA GGAGCAGGAG TACCAGGCTC AGGTGGAGGA GATGAGGCTG 
ATGATGAATC AGCTGGAAGA AGACCTGGTG TCAGCCCGCA GACGCAGCGA TCTCTACGAG 
TCTGAGCTGA GGGAGTCTCG GCTTGCCGCC GAGGAATTCA AGCGGAAGGC AAACGAATGT 
CAGCACAAAC TGATGAAGGT AGTCAGCCAC CCTCCAAGAG GGGACTCCGG GGGCACGGCC 
CTGGATGATC TTCACAAGAT GCAAGGCCAC GCTGGCTTAA CGAGTGCTAA GGACCAAGGG 
AAGCCTGAAG TGGGAGAATA TTCCAAACTG GAGAAGATCA ATGCTGAGCA GCAGCTGAAG 
ATCCAGGAGC TCCAGGAGAA GCTGGAGAAG GCTGTAAAAG CCAGCACAGA GGCCACCGAG 
CTCCTGCAGA ATATCCGCCA GGCAAAGGAG CGAGCAGAGC GGGAGCTAGA GAAGCTACAC 
AACCGGGAAG ACTCCTCCGA GGGCATCAAA AAGAAGCTGG TGGAAGCCGA GGAACGCCGC 
CACTCCCTGG AGAACAAGGT AAAGAGACTA GAGACCATGG AGCGTAGAGA GAACAGACTG 
AAGGATGACA TCCAGACAAA GTCCGAACAG ATCCAGCAGA TGGCTGATAA AATTCTGGAG 
CTGGAGGAGA AACATCGGGA GGCTCAGGTC TCAGCTCAAC ATCTAGAAGT ACACTTGAAA 
CAGAAAGAAC AGCACTACGA GGAAAAGATC AAGGTATTGG ACAATCAGAT AAAGAAGGAC 
CTGGCCGACA AGGAGAGCCT GGAGAACATG ATGCAGAGAC ACGAGGAGGA GGCCCACGAG 
AAGGGCAAGA TCCTCAGCGA GCAGAAGGCG ATGATCAACG CGATGGATTC CAAGATCCGA 
TCCCTGGAGC AGAGGATCGT GGAGCTGTCG GAAGCCAACA AGCTTGCGGC AAACAGCAGT 
CTCTTCACCC AGAGGAACAT GAAGGCCCAG GAAGAGATGA TCTCAGAACT CAGGCAGCAG 
AAATTTTACC TGGAGACGCA GGCCGGAAAG CTGGAGGCCC AGAACCGAAA GCTGGAAGAG 
CAACTGGAGA AGATCAGCCA CCAAGATCAC AGTGACAAGA GTCGGCTGCT GGAGCTGGAA 
ACAAGGCTGA GGGAGGTCAG CCTGGAGCAC GAGGAGCAGA AGCTGGAGCT GAAGCGGCAG 
CTCACGGAGC TGCAGCTGTC CCTGCAGGAG CGCGAGTCCC AGCTGACGGC CCTGCAGGCC 
GCCCGGGCAG CGCTGGAGAG CCAGCTCCGC CAGGCGAAGA CAGAGCTGGA GGAGACAACC 
GCGGAAGCGG AGGAGGAGAT CCAGGCGCTC ACGGCACATC GAGATGAGAT CCAGCGCAAA 
TTCGATGCCC TTCGCAACAG CTGCACGGTC ATCACCGACC TGGAGGAGCA GCTGAACCAG 
CTCACCGAGG ACAACGCCGA GCTCAACAAC CAAAACTTCT ACCTGTCCAA ACAACTCGAT 
GAGGCTTCCG GGGCCAATGA CGAGATTGTG CAGCTGCGGA GTGAGGTGGA CCATCTCCGC 
CGTGAGATCA CGGAGCGGGA GATGCAGCTC ACCAGCCAGA AGCAAGCACA ACTCAGTGCT 
CCTGATTTGC AGACGATGGA GGCTCTGAAG ACGACATGCA CCATGCTGGA GGAGCAGGTC 
CTGGACCTGG AGGCCCTGAA CGACGAGCTG CTGGAGAAGG AGCGCCAGTG GGAGGCCTGG 
CGGAGCGTCC TCGGCGACGA GAAGTCCCAG TTCGAGTGTC GAGTTCGAGA GCTACAGAGG 
ATGCTGGACA CCGAGAAGCA GAGCAGGGCT AGGGCCGATC AGCGGATCAC CGAGTCGCGC 
CAGGTGGTGG AGTTGGCGGT GAAGGAACAC AAGGCCGAGA TTCTTGCTCT GCAGCAGGCT 
CTCAAGGAGC AGAAGCTCAA AGCCGAGAGC CTGTCGGACA AGCTCAACGA CCTGGAGAAG 
AAGCACGCCA TGCTGGAGAT GAACGCCCGG AGCTTACAGC AGAAACTAGA GACAGAGCGG 
GAGCTCAAAC AGAGGCTTCT GGAGGAGCAA GCCAAATTGC AGCAGCAGAT GGACCTGCAG 
AAGAACCACA TCTTCAGACT GACGCAAGGG CTGCAGGAGG CGCTGGACCG GGCGGATCTG 
CTGAAGACAG AAAGGAGCGA CCTGGAGTAC CAGCTGGAAA ACATTCAGGT TCTCTACTCT 
CACGAGAAAG TGAAAATGGA AGGCACAATC TCTCAGCAAA CCAAACTCAT TGATTTCCTG 
CAAGCCAAAA TGGACCAGCC TGCTAAAAAG AAAAAGGGTT TATTTAGTCG ACGGAAAGAG 
GACCCTGCTT TGCCCACACA GGTTCCTCTG CAGTACAATG AGCTGAAGCT AGCCCTGGAA 
AAGGAGAAAG CCCGATGCGC GGAGCTGGAG GAGGCCCTTC AGAAGACCCG CATCGAACTC 
CGCTCTGCGC GGGAGGAAGC TGCCCACCGC AAAGCCACGG ACCACCCGCA CCCGTCTACG 
CCAGCCACTG CGAGGCAGCA GATCGCCATG TCTGCCATTG TGCGGTCGCC CGAGCACCAG 
CCCAGTGCCA TGAGCCTGCT TGCCCCACCA TCCAGCCGCA GAAAGGAGTC GTCAACTCCA 
GAGGAATTCA GCCGGCGTCT GAAAGAGCGC ATGCACCACA ACATCCCTCA CCGGTTTAAT 
GTGGGCCTGA ACATGAGAGC CACCAAGTGC GCCGTGTGTC TGGATACTGT GCACTTTGGA 
CGCCAGGCAT CCAAATGCCT CGAATGTCAA GTGATGTGTC ACCCCAAATG CTCCACCTGC 
TTGCCCGCTA CCTGTGGCCT GCCAGCTGAA TATGCCACAC ACTTCACTGA GGCCTTCTGC 
CGGGACAAAA TGAACTCCCC GGGGCTCCAG AGCAAGGAGC CTGGCAGCAG CTTGCACCTG 
GAAGGGTGGA TGAAAGTGCC CAGAAATAAC AAACGGGGAC AGCAAGGCTG GGACAGGAAG 
TACATTGTTC TGGAGGGGTC AAAAGTCCTC ATCTATGACA ATGAAGCCAG AGAAGCTGGA 
CAGAGGCCGG TGGAAGAATT TGAGCTGTGC CTTCCTGACG GGGACGTATC TATTCATGGT 
GCCGTTGGTG CTTCAGAACT TGCAAATACC GCCAAAGCAG ATGTCCCATA CATCCTGAAG 
ATGGAGTCTC ACCCACACAC CACCTGCTGG CCTGGGAGAA CCCTCTACTT GCTAGCACCC 
AGCTTCCCCG ACAAGCAGCG CTGGGTCACC GCCTTAGAAT CTGTCGTCGC AGGTGGGAGA 
GTTTCTAGGG AAAAGGCCGA AGCCGATGCT GCACGTGACC ATACTTCGAG TGAGCATCAG 
CCAGTGTGGG TTGAGAAATT ACTTGGAAAC TCTCTGCTGA AACTGGAGGG CGATGACCGG 
CTTGACATGA ACTGCACCCT GCCCTTCAGT GACCAGGTAG TGCTGGTGGG CACGGAGGAA 
GGCCTCTACG CCCTGAATGT CTTGAAAAAC TCCTTAACCC ACATCCCAGG GATTGGCGCA 
GTCTTCCAAA TTTACATCAT CAAGGACCTG GAGAAGCTGC TCATGATAGC AGGGGAAGAG 
CGGGCTCTGT GCCTGGTGGA CGTGAAGAAG GTGAAGCAGT CCCTGGCGCA GTCACACCTG 
CCTGCCCAGC CCGACGTCTC CCCCAACATA TTCGAAGCCG TCAAAGGCTG CCACTTGTTC 
GCTGCTGGCA AGATCGAGAA CAGCCTGTGC ATCTGCGCCG CTATGCCAAG CAAAGTCGTC 
ATCCTCCGCT ACAATGACAA CCTCAGCAAG TACTGCATCC GCAAGGAGAT CGAGACCTCA 
GAGCCCTGCA GCTGTATCCA CTTCACCAAC TACAGCATCC TCATTGGGAC CAACAAATTC 
TATGAGATCG ACATGAAGCA GTACACGCTT GACGAGTTCC TGGACAAGAA CGACCATTCC 
TTGGCACCTG CTGTGTTCGC CTCCTCGTCC AACAGCTTCC CTGTCTCCAT TGTGCAGGCG 
AACAGCGCCG GGCAGCGAGA AGAATACCTG CTGTGCTTCC ACGAATTTGG GGTGTTCGTG 
GATTCTTACG GAAGACGTAG CCGCACAGAT GATCTCAAGT GGAGTCGCTT ACCTCTGGCC 
TTCGCCTACA GAGAACCTTA TCTGTTTGTG ACTCACTTCA ACTCCCTGGA AGTCATTGAG 
ATCCAGGCCA GATCCTCACT GGGGAGCCCT GCCCGAGCAT ATCTGGAAAT TCCAAACCCT 
CGCTACCTGG GCCCCGCGAT TTCCTCCGGA GCGATTTACC TGGCCTCCTC ATACCAGGAC 
AAGTTAAGGG TCATATGCTG CAAAGGAAAC CTCGTGAAGG AGTCAGGCAC TGAGCAGCAC 
CGGGTGCCCT CCACCTCCCG CAGCAGCCCC AACAAGCGAG GCCCACCAAC ATACAACGAG 
CACATCACCA AACGCGTGGC CTCCAGCCCG GCGCCACCGG AAGGCCCCAG CCACCCCCGA 
GAGCCAAGCA CACCGCACCG CTACCGAGAC AGAGAGGGTC GGACAGAGCT GCGCAGGGAC 
AAGTCTCCAG GCCGCCCTCT GGAGCGGGAG AAGTCCCCAG GCCGAATGCT CAGCACTAGG 
AGAGAGCGGT CCCCAGGGAG ACTGTTTGAA GACAGCAGCA GGGGCCGGCT GCCTGCAGGA 
GCAGTGAGGA CCCCACTGTC CCAGGTTAAC AAGGTCTGGG ACCAGTCTTC AGTATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cit ( XM_006530147.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006530210.1 Mus musculus citron (Cit), transcript variant X19, mRNA.

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CloneID	OMu35787
Clone ID Related Accession (Same CDS sequence)	XM_006530148.3 , XM_006530147.2 , XM_006530148.4 , XM_006530147.1
Accession Version	XM_006530147.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5037bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	citron Rho-interacting kinase isoform X16
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000071.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAAAGA AACTTCTCAT CAAAAGCAAA GAGCTCCAAG ACTCCCAGGA CAAGTGTCAC 
AAGATGGAGC AGGAAATGAC CCGGTTACAT CGCAGAGTGT CAGAGGTGGA GGCTGTGCTT 
AGTCAGAAGG AGGTGGAGCT GAAGGCCTCT GAGACTCAGA GATCCCTCCT GGAGCAGGAC 
CTTGCTACCT ACATCACAGA ATGCAGTAGC TTAAAGCGAA GTTTGGAGCA AGCGCGGATG 
GAGGTGTCCC AGGAGGATGA CAAAGCTCTG CAGCTTCTCC ACGACATCCG AGAGCAGAGC 
CGGAAGCTCC AGGAGATCAA GGAGCAGGAG TACCAGGCTC AGGTGGAGGA GATGAGGCTG 
ATGATGAATC AGCTGGAAGA AGACCTGGTG TCAGCCCGCA GACGCAGCGA TCTCTACGAG 
TCTGAGCTGA GGGAGTCTCG GCTTGCCGCC GAGGAATTCA AGCGGAAGGC AAACGAATGT 
CAGCACAAAC TGATGAAGGT AGTCAGCCAC CCTCCAAGAG GGGACTCCGG GGGCACGGCC 
CTGGATGATC TTCACAAGAT GCAAGGCCAC GCTGGCTTAA CGAGTGCTAA GGACCAAGGG 
AAGCCTGAAG TGGGAGAATA TTCCAAACTG GAGAAGATCA ATGCTGAGCA GCAGCTGAAG 
ATCCAGGAGC TCCAGGAGAA GCTGGAGAAG GCTGTAAAAG CCAGCACAGA GGCCACCGAG 
CTCCTGCAGA ATATCCGCCA GGCAAAGGAG CGAGCAGAGC GGGAGCTAGA GAAGCTACAC 
AACCGGGAAG ACTCCTCCGA GGGCATCAAA AAGAAGCTGG TGGAAGCCGA GGAACGCCGC 
CACTCCCTGG AGAACAAGGT AAAGAGACTA GAGACCATGG AGCGTAGAGA GAACAGACTG 
AAGGATGACA TCCAGACAAA GTCCGAACAG ATCCAGCAGA TGGCTGATAA AATTCTGGAG 
CTGGAGGAGA AACATCGGGA GGCTCAGGTC TCAGCTCAAC ATCTAGAAGT ACACTTGAAA 
CAGAAAGAAC AGCACTACGA GGAAAAGATC AAGGTATTGG ACAATCAGAT AAAGAAGGAC 
CTGGCCGACA AGGAGAGCCT GGAGAACATG ATGCAGAGAC ACGAGGAGGA GGCCCACGAG 
AAGGGCAAGA TCCTCAGCGA GCAGAAGGCG ATGATCAACG CGATGGATTC CAAGATCCGA 
TCCCTGGAGC AGAGGATCGT GGAGCTGTCG GAAGCCAACA AGCTTGCGGC AAACAGCAGT 
CTCTTCACCC AGAGGAACAT GAAGGCCCAG GAAGAGATGA TCTCAGAACT CAGGCAGCAG 
AAATTTTACC TGGAGACGCA GGCCGGAAAG CTGGAGGCCC AGAACCGAAA GCTGGAAGAG 
CAACTGGAGA AGATCAGCCA CCAAGATCAC AGTGACAAGA GTCGGCTGCT GGAGCTGGAA 
ACAAGGCTGA GGGAGGTCAG CCTGGAGCAC GAGGAGCAGA AGCTGGAGCT GAAGCGGCAG 
CTCACGGAGC TGCAGCTGTC CCTGCAGGAG CGCGAGTCCC AGCTGACGGC CCTGCAGGCC 
GCCCGGGCAG CGCTGGAGAG CCAGCTCCGC CAGGCGAAGA CAGAGCTGGA GGAGACAACC 
GCGGAAGCGG AGGAGGAGAT CCAGGCGCTC ACGGCACATC GAGATGAGAT CCAGCGCAAA 
TTCGATGCCC TTCGCAACAG CTGCACGGTC ATCACCGACC TGGAGGAGCA GCTGAACCAG 
CTCACCGAGG ACAACGCCGA GCTCAACAAC CAAAACTTCT ACCTGTCCAA ACAACTCGAT 
GAGGCTTCCG GGGCCAATGA CGAGATTGTG CAGCTGCGGA GTGAGGTGGA CCATCTCCGC 
CGTGAGATCA CGGAGCGGGA GATGCAGCTC ACCAGCCAGA AGCAAGCACA ACTCAGTGCT 
CCTGATTTGC AGACGATGGA GGCTCTGAAG ACGACATGCA CCATGCTGGA GGAGCAGGTC 
CTGGACCTGG AGGCCCTGAA CGACGAGCTG CTGGAGAAGG AGCGCCAGTG GGAGGCCTGG 
CGGAGCGTCC TCGGCGACGA GAAGTCCCAG TTCGAGTGTC GAGTTCGAGA GCTACAGAGG 
ATGCTGGACA CCGAGAAGCA GAGCAGGGCT AGGGCCGATC AGCGGATCAC CGAGTCGCGC 
CAGGTGGTGG AGTTGGCGGT GAAGGAACAC AAGGCCGAGA TTCTTGCTCT GCAGCAGGCT 
CTCAAGGAGC AGAAGCTCAA AGCCGAGAGC CTGTCGGACA AGCTCAACGA CCTGGAGAAG 
AAGCACGCCA TGCTGGAGAT GAACGCCCGG AGCTTACAGC AGAAACTAGA GACAGAGCGG 
GAGCTCAAAC AGAGGCTTCT GGAGGAGCAA GCCAAATTGC AGCAGCAGAT GGACCTGCAG 
AAGAACCACA TCTTCAGACT GACGCAAGGG CTGCAGGAGG CGCTGGACCG GGCGGATCTG 
CTGAAGACAG AAAGGAGCGA CCTGGAGTAC CAGCTGGAAA ACATTCAGGT TCTCTACTCT 
CACGAGAAAG TGAAAATGGA AGGCACAATC TCTCAGCAAA CCAAACTCAT TGATTTCCTG 
CAAGCCAAAA TGGACCAGCC TGCTAAAAAG AAAAAGGGTT TATTTAGTCG ACGGAAAGAG 
GACCCTGCTT TGCCCACACA GGTTCCTCTG CAGTACAATG AGCTGAAGCT AGCCCTGGAA 
AAGGAGAAAG CCCGATGCGC GGAGCTGGAG GAGGCCCTTC AGAAGACCCG CATCGAACTC 
CGCTCTGCGC GGGAGGAAGC TGCCCACCGC AAAGCCACGG ACCACCCGCA CCCGTCTACG 
CCAGCCACTG CGAGGCAGCA GATCGCCATG TCTGCCATTG TGCGGTCGCC CGAGCACCAG 
CCCAGTGCCA TGAGCCTGCT TGCCCCACCA TCCAGCCGCA GAAAGGAGTC GTCAACTCCA 
GAGGAATTCA GCCGGCGTCT GAAAGAGCGC ATGCACCACA ACATCCCTCA CCGGTTTAAT 
GTGGGCCTGA ACATGAGAGC CACCAAGTGC GCCGTGTGTC TGGATACTGT GCACTTTGGA 
CGCCAGGCAT CCAAATGCCT CGAATGTCAA GTGATGTGTC ACCCCAAATG CTCCACCTGC 
TTGCCCGCTA CCTGTGGCCT GCCAGCTGAA TATGCCACAC ACTTCACTGA GGCCTTCTGC 
CGGGACAAAA TGAACTCCCC GGGGCTCCAG AGCAAGGAGC CTGGCAGCAG CTTGCACCTG 
GAAGGGTGGA TGAAAGTGCC CAGAAATAAC AAACGGGGAC AGCAAGGCTG GGACAGGAAG 
TACATTGTTC TGGAGGGGTC AAAAGTCCTC ATCTATGACA ATGAAGCCAG AGAAGCTGGA 
CAGAGGCCGG TGGAAGAATT TGAGCTGTGC CTTCCTGACG GGGACGTATC TATTCATGGT 
GCCGTTGGTG CTTCAGAACT TGCAAATACC GCCAAAGCAG ATGTCCCATA CATCCTGAAG 
ATGGAGTCTC ACCCACACAC CACCTGCTGG CCTGGGAGAA CCCTCTACTT GCTAGCACCC 
AGCTTCCCCG ACAAGCAGCG CTGGGTCACC GCCTTAGAAT CTGTCGTCGC AGGTGGGAGA 
GTTTCTAGGG AAAAGGCCGA AGCCGATGCT GCACGTGACC ATACTTCGAG TGAGCATCAG 
CCAGTGTGGG TTGAGAAATT ACTTGGAAAC TCTCTGCTGA AACTGGAGGG CGATGACCGG 
CTTGACATGA ACTGCACCCT GCCCTTCAGT GACCAGGTAG TGCTGGTGGG CACGGAGGAA 
GGCCTCTACG CCCTGAATGT CTTGAAAAAC TCCTTAACCC ACATCCCAGG GATTGGCGCA 
GTCTTCCAAA TTTACATCAT CAAGGACCTG GAGAAGCTGC TCATGATAGC AGGGGAAGAG 
CGGGCTCTGT GCCTGGTGGA CGTGAAGAAG GTGAAGCAGT CCCTGGCGCA GTCACACCTG 
CCTGCCCAGC CCGACGTCTC CCCCAACATA TTCGAAGCCG TCAAAGGCTG CCACTTGTTC 
GCTGCTGGCA AGATCGAGAA CAGCCTGTGC ATCTGCGCCG CTATGCCAAG CAAAGTCGTC 
ATCCTCCGCT ACAATGACAA CCTCAGCAAG TACTGCATCC GCAAGGAGAT CGAGACCTCA 
GAGCCCTGCA GCTGTATCCA CTTCACCAAC TACAGCATCC TCATTGGGAC CAACAAATTC 
TATGAGATCG ACATGAAGCA GTACACGCTT GACGAGTTCC TGGACAAGAA CGACCATTCC 
TTGGCACCTG CTGTGTTCGC CTCCTCGTCC AACAGCTTCC CTGTCTCCAT TGTGCAGGCG 
AACAGCGCCG GGCAGCGAGA AGAATACCTG CTGTGCTTCC ACGAATTTGG GGTGTTCGTG 
GATTCTTACG GAAGACGTAG CCGCACAGAT GATCTCAAGT GGAGTCGCTT ACCTCTGGCC 
TTCGCCTACA GAGAACCTTA TCTGTTTGTG ACTCACTTCA ACTCCCTGGA AGTCATTGAG 
ATCCAGGCCA GATCCTCACT GGGGAGCCCT GCCCGAGCAT ATCTGGAAAT TCCAAACCCT 
CGCTACCTGG GCCCCGCGAT TTCCTCCGGA GCGATTTACC TGGCCTCCTC ATACCAGGAC 
AAGTTAAGGG TCATATGCTG CAAAGGAAAC CTCGTGAAGG AGTCAGGCAC TGAGCAGCAC 
CGGGTGCCCT CCACCTCCCG CAGCAGCCCC AACAAGCGAG GCCCACCAAC ATACAACGAG 
CACATCACCA AACGCGTGGC CTCCAGCCCG GCGCCACCGG AAGGCCCCAG CCACCCCCGA 
GAGCCAAGCA CACCGCACCG CTACCGAGAC AGAGAGGGTC GGACAGAGCT GCGCAGGGAC 
AAGTCTCCAG GCCGCCCTCT GGAGCGGGAG AAGTCCCCAG GCCGAATGCT CAGCACTAGG 
AGAGAGCGGT CCCCAGGGAG ACTGTTTGAA GACAGCAGCA GGGGCCGGCT GCCTGCAGGA 
GCAGTGAGGA CCCCACTGTC CCAGGTTAAC AAGGTCTGGG ACCAGTCTTC AGTATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006530210.1
CDS	588..5624
Translation	MEKKLLIKSKELQDSQDKCHKMEQEMTRLHRRVSEVEAVLSQKEVELKASETQRSLLEQDLATYITECSSLKRSLEQARMEVSQEDDKALQLLHDIREQSRKLQEIKEQEYQAQVEEMRLMMNQLEEDLVSARRRSDLYESELRESRLAAEEFKRKANECQHKLMKVVSHPPRGDSGGTALDDLHKMQGHAGLTSAKDQGKPEVGEYSKLEKINAEQQLKIQELQEKLEKAVKASTEATELLQNIRQAKERAERELEKLHNREDSSEGIKKKLVEAEERRHSLENKVKRLETMERRENRLKDDIQTKSEQIQQMADKILELEEKHREAQVSAQHLEVHLKQKEQHYEEKIKVLDNQIKKDLADKESLENMMQRHEEEAHEKGKILSEQKAMINAMDSKIRSLEQRIVELSEANKLAANSSLFTQRNMKAQEEMISELRQQKFYLETQAGKLEAQNRKLEEQLEKISHQDHSDKSRLLELETRLREVSLEHEEQKLELKRQLTELQLSLQERESQLTALQAARAALESQLRQAKTELEETTAEAEEEIQALTAHRDEIQRKFDALRNSCTVITDLEEQLNQLTEDNAELNNQNFYLSKQLDEASGANDEIVQLRSEVDHLRREITEREMQLTSQKQAQLSAPDLQTMEALKTTCTMLEEQVLDLEALNDELLEKERQWEAWRSVLGDEKSQFECRVRELQRMLDTEKQSRARADQRITESRQVVELAVKEHKAEILALQQALKEQKLKAESLSDKLNDLEKKHAMLEMNARSLQQKLETERELKQRLLEEQAKLQQQMDLQKNHIFRLTQGLQEALDRADLLKTERSDLEYQLENIQVLYSHEKVKMEGTISQQTKLIDFLQAKMDQPAKKKKGLFSRRKEDPALPTQVPLQYNELKLALEKEKARCAELEEALQKTRIELRSAREEAAHRKATDHPHPSTPATARQQIAMSAIVRSPEHQPSAMSLLAPPSSRRKESSTPEEFSRRLKERMHHNIPHRFNVGLNMRATKCAVCLDTVHFGRQASKCLECQVMCHPKCSTCLPATCGLPAEYATHFTEAFCRDKMNSPGLQSKEPGSSLHLEGWMKVPRNNKRGQQGWDRKYIVLEGSKVLIYDNEAREAGQRPVEEFELCLPDGDVSIHGAVGASELANTAKADVPYILKMESHPHTTCWPGRTLYLLAPSFPDKQRWVTALESVVAGGRVSREKAEADAARDHTSSEHQPVWVEKLLGNSLLKLEGDDRLDMNCTLPFSDQVVLVGTEEGLYALNVLKNSLTHIPGIGAVFQIYIIKDLEKLLMIAGEERALCLVDVKKVKQSLAQSHLPAQPDVSPNIFEAVKGCHLFAAGKIENSLCICAAMPSKVVILRYNDNLSKYCIRKEIETSEPCSCIHFTNYSILIGTNKFYEIDMKQYTLDEFLDKNDHSLAPAVFASSSNSFPVSIVQANSAGQREEYLLCFHEFGVFVDSYGRRSRTDDLKWSRLPLAFAYREPYLFVTHFNSLEVIEIQARSSLGSPARAYLEIPNPRYLGPAISSGAIYLASSYQDKLRVICCKGNLVKESGTEQHRVPSTSRSSPNKRGPPTYNEHITKRVASSPAPPEGPSHPREPSTPHRYRDREGRTELRRDKSPGRPLEREKSPGRMLSTRRERSPGRLFEDSSRGRLPAGAVRTPLSQVNKVWDQSSV

Target ORF information:

RefSeq Version	XM_006530147.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus citron (Cit), transcript variant X19, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006530147.1

ORF Insert Sequence:

ATGGAAAAGA AACTTCTCAT CAAAAGCAAA GAGCTCCAAG ACTCCCAGGA CAAGTGTCAC 
AAGATGGAGC AGGAAATGAC CCGGTTACAT CGCAGAGTGT CAGAGGTGGA GGCTGTGCTT 
AGTCAGAAGG AGGTGGAGCT GAAGGCCTCT GAGACTCAGA GATCCCTCCT GGAGCAGGAC 
CTTGCTACCT ACATCACAGA ATGCAGTAGC TTAAAGCGAA GTTTGGAGCA AGCGCGGATG 
GAGGTGTCCC AGGAGGATGA CAAAGCTCTG CAGCTTCTCC ACGACATCCG AGAGCAGAGC 
CGGAAGCTCC AGGAGATCAA GGAGCAGGAG TACCAGGCTC AGGTGGAGGA GATGAGGCTG 
ATGATGAATC AGCTGGAAGA AGACCTGGTG TCAGCCCGCA GACGCAGCGA TCTCTACGAG 
TCTGAGCTGA GGGAGTCTCG GCTTGCCGCC GAGGAATTCA AGCGGAAGGC AAACGAATGT 
CAGCACAAAC TGATGAAGGT AGTCAGCCAC CCTCCAAGAG GGGACTCCGG GGGCACGGCC 
CTGGATGATC TTCACAAGAT GCAAGGCCAC GCTGGCTTAA CGAGTGCTAA GGACCAAGGG 
AAGCCTGAAG TGGGAGAATA TTCCAAACTG GAGAAGATCA ATGCTGAGCA GCAGCTGAAG 
ATCCAGGAGC TCCAGGAGAA GCTGGAGAAG GCTGTAAAAG CCAGCACAGA GGCCACCGAG 
CTCCTGCAGA ATATCCGCCA GGCAAAGGAG CGAGCAGAGC GGGAGCTAGA GAAGCTACAC 
AACCGGGAAG ACTCCTCCGA GGGCATCAAA AAGAAGCTGG TGGAAGCCGA GGAACGCCGC 
CACTCCCTGG AGAACAAGGT AAAGAGACTA GAGACCATGG AGCGTAGAGA GAACAGACTG 
AAGGATGACA TCCAGACAAA GTCCGAACAG ATCCAGCAGA TGGCTGATAA AATTCTGGAG 
CTGGAGGAGA AACATCGGGA GGCTCAGGTC TCAGCTCAAC ATCTAGAAGT ACACTTGAAA 
CAGAAAGAAC AGCACTACGA GGAAAAGATC AAGGTATTGG ACAATCAGAT AAAGAAGGAC 
CTGGCCGACA AGGAGAGCCT GGAGAACATG ATGCAGAGAC ACGAGGAGGA GGCCCACGAG 
AAGGGCAAGA TCCTCAGCGA GCAGAAGGCG ATGATCAACG CGATGGATTC CAAGATCCGA 
TCCCTGGAGC AGAGGATCGT GGAGCTGTCG GAAGCCAACA AGCTTGCGGC AAACAGCAGT 
CTCTTCACCC AGAGGAACAT GAAGGCCCAG GAAGAGATGA TCTCAGAACT CAGGCAGCAG 
AAATTTTACC TGGAGACGCA GGCCGGAAAG CTGGAGGCCC AGAACCGAAA GCTGGAAGAG 
CAACTGGAGA AGATCAGCCA CCAAGATCAC AGTGACAAGA GTCGGCTGCT GGAGCTGGAA 
ACAAGGCTGA GGGAGGTCAG CCTGGAGCAC GAGGAGCAGA AGCTGGAGCT GAAGCGGCAG 
CTCACGGAGC TGCAGCTGTC CCTGCAGGAG CGCGAGTCCC AGCTGACGGC CCTGCAGGCC 
GCCCGGGCAG CGCTGGAGAG CCAGCTCCGC CAGGCGAAGA CAGAGCTGGA GGAGACAACC 
GCGGAAGCGG AGGAGGAGAT CCAGGCGCTC ACGGCACATC GAGATGAGAT CCAGCGCAAA 
TTCGATGCCC TTCGCAACAG CTGCACGGTC ATCACCGACC TGGAGGAGCA GCTGAACCAG 
CTCACCGAGG ACAACGCCGA GCTCAACAAC CAAAACTTCT ACCTGTCCAA ACAACTCGAT 
GAGGCTTCCG GGGCCAATGA CGAGATTGTG CAGCTGCGGA GTGAGGTGGA CCATCTCCGC 
CGTGAGATCA CGGAGCGGGA GATGCAGCTC ACCAGCCAGA AGCAAGCACA ACTCAGTGCT 
CCTGATTTGC AGACGATGGA GGCTCTGAAG ACGACATGCA CCATGCTGGA GGAGCAGGTC 
CTGGACCTGG AGGCCCTGAA CGACGAGCTG CTGGAGAAGG AGCGCCAGTG GGAGGCCTGG 
CGGAGCGTCC TCGGCGACGA GAAGTCCCAG TTCGAGTGTC GAGTTCGAGA GCTACAGAGG 
ATGCTGGACA CCGAGAAGCA GAGCAGGGCT AGGGCCGATC AGCGGATCAC CGAGTCGCGC 
CAGGTGGTGG AGTTGGCGGT GAAGGAACAC AAGGCCGAGA TTCTTGCTCT GCAGCAGGCT 
CTCAAGGAGC AGAAGCTCAA AGCCGAGAGC CTGTCGGACA AGCTCAACGA CCTGGAGAAG 
AAGCACGCCA TGCTGGAGAT GAACGCCCGG AGCTTACAGC AGAAACTAGA GACAGAGCGG 
GAGCTCAAAC AGAGGCTTCT GGAGGAGCAA GCCAAATTGC AGCAGCAGAT GGACCTGCAG 
AAGAACCACA TCTTCAGACT GACGCAAGGG CTGCAGGAGG CGCTGGACCG GGCGGATCTG 
CTGAAGACAG AAAGGAGCGA CCTGGAGTAC CAGCTGGAAA ACATTCAGGT TCTCTACTCT 
CACGAGAAAG TGAAAATGGA AGGCACAATC TCTCAGCAAA CCAAACTCAT TGATTTCCTG 
CAAGCCAAAA TGGACCAGCC TGCTAAAAAG AAAAAGGGTT TATTTAGTCG ACGGAAAGAG 
GACCCTGCTT TGCCCACACA GGTTCCTCTG CAGTACAATG AGCTGAAGCT AGCCCTGGAA 
AAGGAGAAAG CCCGATGCGC GGAGCTGGAG GAGGCCCTTC AGAAGACCCG CATCGAACTC 
CGCTCTGCGC GGGAGGAAGC TGCCCACCGC AAAGCCACGG ACCACCCGCA CCCGTCTACG 
CCAGCCACTG CGAGGCAGCA GATCGCCATG TCTGCCATTG TGCGGTCGCC CGAGCACCAG 
CCCAGTGCCA TGAGCCTGCT TGCCCCACCA TCCAGCCGCA GAAAGGAGTC GTCAACTCCA 
GAGGAATTCA GCCGGCGTCT GAAAGAGCGC ATGCACCACA ACATCCCTCA CCGGTTTAAT 
GTGGGCCTGA ACATGAGAGC CACCAAGTGC GCCGTGTGTC TGGATACTGT GCACTTTGGA 
CGCCAGGCAT CCAAATGCCT CGAATGTCAA GTGATGTGTC ACCCCAAATG CTCCACCTGC 
TTGCCCGCTA CCTGTGGCCT GCCAGCTGAA TATGCCACAC ACTTCACTGA GGCCTTCTGC 
CGGGACAAAA TGAACTCCCC GGGGCTCCAG AGCAAGGAGC CTGGCAGCAG CTTGCACCTG 
GAAGGGTGGA TGAAAGTGCC CAGAAATAAC AAACGGGGAC AGCAAGGCTG GGACAGGAAG 
TACATTGTTC TGGAGGGGTC AAAAGTCCTC ATCTATGACA ATGAAGCCAG AGAAGCTGGA 
CAGAGGCCGG TGGAAGAATT TGAGCTGTGC CTTCCTGACG GGGACGTATC TATTCATGGT 
GCCGTTGGTG CTTCAGAACT TGCAAATACC GCCAAAGCAG ATGTCCCATA CATCCTGAAG 
ATGGAGTCTC ACCCACACAC CACCTGCTGG CCTGGGAGAA CCCTCTACTT GCTAGCACCC 
AGCTTCCCCG ACAAGCAGCG CTGGGTCACC GCCTTAGAAT CTGTCGTCGC AGGTGGGAGA 
GTTTCTAGGG AAAAGGCCGA AGCCGATGCT GCACGTGACC ATACTTCGAG TGAGCATCAG 
CCAGTGTGGG TTGAGAAATT ACTTGGAAAC TCTCTGCTGA AACTGGAGGG CGATGACCGG 
CTTGACATGA ACTGCACCCT GCCCTTCAGT GACCAGGTAG TGCTGGTGGG CACGGAGGAA 
GGCCTCTACG CCCTGAATGT CTTGAAAAAC TCCTTAACCC ACATCCCAGG GATTGGCGCA 
GTCTTCCAAA TTTACATCAT CAAGGACCTG GAGAAGCTGC TCATGATAGC AGGGGAAGAG 
CGGGCTCTGT GCCTGGTGGA CGTGAAGAAG GTGAAGCAGT CCCTGGCGCA GTCACACCTG 
CCTGCCCAGC CCGACGTCTC CCCCAACATA TTCGAAGCCG TCAAAGGCTG CCACTTGTTC 
GCTGCTGGCA AGATCGAGAA CAGCCTGTGC ATCTGCGCCG CTATGCCAAG CAAAGTCGTC 
ATCCTCCGCT ACAATGACAA CCTCAGCAAG TACTGCATCC GCAAGGAGAT CGAGACCTCA 
GAGCCCTGCA GCTGTATCCA CTTCACCAAC TACAGCATCC TCATTGGGAC CAACAAATTC 
TATGAGATCG ACATGAAGCA GTACACGCTT GACGAGTTCC TGGACAAGAA CGACCATTCC 
TTGGCACCTG CTGTGTTCGC CTCCTCGTCC AACAGCTTCC CTGTCTCCAT TGTGCAGGCG 
AACAGCGCCG GGCAGCGAGA AGAATACCTG CTGTGCTTCC ACGAATTTGG GGTGTTCGTG 
GATTCTTACG GAAGACGTAG CCGCACAGAT GATCTCAAGT GGAGTCGCTT ACCTCTGGCC 
TTCGCCTACA GAGAACCTTA TCTGTTTGTG ACTCACTTCA ACTCCCTGGA AGTCATTGAG 
ATCCAGGCCA GATCCTCACT GGGGAGCCCT GCCCGAGCAT ATCTGGAAAT TCCAAACCCT 
CGCTACCTGG GCCCCGCGAT TTCCTCCGGA GCGATTTACC TGGCCTCCTC ATACCAGGAC 
AAGTTAAGGG TCATATGCTG CAAAGGAAAC CTCGTGAAGG AGTCAGGCAC TGAGCAGCAC 
CGGGTGCCCT CCACCTCCCG CAGCAGCCCC AACAAGCGAG GCCCACCAAC ATACAACGAG 
CACATCACCA AACGCGTGGC CTCCAGCCCG GCGCCACCGG AAGGCCCCAG CCACCCCCGA 
GAGCCAAGCA CACCGCACCG CTACCGAGAC AGAGAGGGTC GGACAGAGCT GCGCAGGGAC 
AAGTCTCCAG GCCGCCCTCT GGAGCGGGAG AAGTCCCCAG GCCGAATGCT CAGCACTAGG 
AGAGAGCGGT CCCCAGGGAG ACTGTTTGAA GACAGCAGCA GGGGCCGGCT GCCTGCAGGA 
GCAGTGAGGA CCCCACTGTC CCAGGTTAAC AAGGTCTGGG ACCAGTCTTC AGTATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cit ( XM_006530149.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006530212.1 Mus musculus citron (Cit), transcript variant X21, mRNA.

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CloneID	OMu35788
Clone ID Related Accession (Same CDS sequence)	XM_006530149.4 , XM_006530149.3
Accession Version	XM_006530149.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4998bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	citron Rho-interacting kinase isoform X17
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000071.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006530149.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTCCTGG GCGAAGAAGC AATGATGGAG CAGGAAATGA CCCGGTTACA TCGCAGAGTG 
TCAGAGGTGG AGGCTGTGCT TAGTCAGAAG GAGGTGGAGC TGAAGGCCTC TGAGACTCAG 
AGATCCCTCC TGGAGCAGGA CCTTGCTACC TACATCACAG AATGCAGTAG CTTAAAGCGA 
AGTTTGGAGC AAGCGCGGAT GGAGGTGTCC CAGGAGGATG ACAAAGCTCT GCAGCTTCTC 
CACGACATCC GAGAGCAGAG CCGGAAGCTC CAGGAGATCA AGGAGCAGGA GTACCAGGCT 
CAGGTGGAGG AGATGAGGCT GATGATGAAT CAGCTGGAAG AAGACCTGGT GTCAGCCCGC 
AGACGCAGCG ATCTCTACGA GTCTGAGCTG AGGGAGTCTC GGCTTGCCGC CGAGGAATTC 
AAGCGGAAGG CAAACGAATG TCAGCACAAA CTGATGAAGG TAGTCAGCCA CCCTCCAAGA 
GGGGACTCCG GGGGCACGGC CCTGGATGAT CTTCACAAGA TGCAAGGCCA CGCTGGCTTA 
ACGAGTGCTA AGGACCAAGG GAAGCCTGAA GTGGGAGAAT ATTCCAAACT GGAGAAGATC 
AATGCTGAGC AGCAGCTGAA GATCCAGGAG CTCCAGGAGA AGCTGGAGAA GGCTGTAAAA 
GCCAGCACAG AGGCCACCGA GCTCCTGCAG AATATCCGCC AGGCAAAGGA GCGAGCAGAG 
CGGGAGCTAG AGAAGCTACA CAACCGGGAA GACTCCTCCG AGGGCATCAA AAAGAAGCTG 
GTGGAAGCCG AGGAACGCCG CCACTCCCTG GAGAACAAGG TAAAGAGACT AGAGACCATG 
GAGCGTAGAG AGAACAGACT GAAGGATGAC ATCCAGACAA AGTCCGAACA GATCCAGCAG 
ATGGCTGATA AAATTCTGGA GCTGGAGGAG AAACATCGGG AGGCTCAGGT CTCAGCTCAA 
CATCTAGAAG TACACTTGAA ACAGAAAGAA CAGCACTACG AGGAAAAGAT CAAGGTATTG 
GACAATCAGA TAAAGAAGGA CCTGGCCGAC AAGGAGAGCC TGGAGAACAT GATGCAGAGA 
CACGAGGAGG AGGCCCACGA GAAGGGCAAG ATCCTCAGCG AGCAGAAGGC GATGATCAAC 
GCGATGGATT CCAAGATCCG ATCCCTGGAG CAGAGGATCG TGGAGCTGTC GGAAGCCAAC 
AAGCTTGCGG CAAACAGCAG TCTCTTCACC CAGAGGAACA TGAAGGCCCA GGAAGAGATG 
ATCTCAGAAC TCAGGCAGCA GAAATTTTAC CTGGAGACGC AGGCCGGAAA GCTGGAGGCC 
CAGAACCGAA AGCTGGAAGA GCAACTGGAG AAGATCAGCC ACCAAGATCA CAGTGACAAG 
AGTCGGCTGC TGGAGCTGGA AACAAGGCTG AGGGAGGTCA GCCTGGAGCA CGAGGAGCAG 
AAGCTGGAGC TGAAGCGGCA GCTCACGGAG CTGCAGCTGT CCCTGCAGGA GCGCGAGTCC 
CAGCTGACGG CCCTGCAGGC CGCCCGGGCA GCGCTGGAGA GCCAGCTCCG CCAGGCGAAG 
ACAGAGCTGG AGGAGACAAC CGCGGAAGCG GAGGAGGAGA TCCAGGCGCT CACGGCACAT 
CGAGATGAGA TCCAGCGCAA ATTCGATGCC CTTCGCAACA GCTGCACGGT CATCACCGAC 
CTGGAGGAGC AGCTGAACCA GCTCACCGAG GACAACGCCG AGCTCAACAA CCAAAACTTC 
TACCTGTCCA AACAACTCGA TGAGGCTTCC GGGGCCAATG ACGAGATTGT GCAGCTGCGG 
AGTGAGGTGG ACCATCTCCG CCGTGAGATC ACGGAGCGGG AGATGCAGCT CACCAGCCAG 
AAGCAAGCAC AACTCAGTGC TCCTGATTTG CAGACGATGG AGGCTCTGAA GACGACATGC 
ACCATGCTGG AGGAGCAGGT CCTGGACCTG GAGGCCCTGA ACGACGAGCT GCTGGAGAAG 
GAGCGCCAGT GGGAGGCCTG GCGGAGCGTC CTCGGCGACG AGAAGTCCCA GTTCGAGTGT 
CGAGTTCGAG AGCTACAGAG GATGCTGGAC ACCGAGAAGC AGAGCAGGGC TAGGGCCGAT 
CAGCGGATCA CCGAGTCGCG CCAGGTGGTG GAGTTGGCGG TGAAGGAACA CAAGGCCGAG 
ATTCTTGCTC TGCAGCAGGC TCTCAAGGAG CAGAAGCTCA AAGCCGAGAG CCTGTCGGAC 
AAGCTCAACG ACCTGGAGAA GAAGCACGCC ATGCTGGAGA TGAACGCCCG GAGCTTACAG 
CAGAAACTAG AGACAGAGCG GGAGCTCAAA CAGAGGCTTC TGGAGGAGCA AGCCAAATTG 
CAGCAGCAGA TGGACCTGCA GAAGAACCAC ATCTTCAGAC TGACGCAAGG GCTGCAGGAG 
GCGCTGGACC GGGCGGATCT GCTGAAGACA GAAAGGAGCG ACCTGGAGTA CCAGCTGGAA 
AACATTCAGG TTCTCTACTC TCACGAGAAA GTGAAAATGG AAGGCACAAT CTCTCAGCAA 
ACCAAACTCA TTGATTTCCT GCAAGCCAAA ATGGACCAGC CTGCTAAAAA GAAAAAGGGT 
TTATTTAGTC GACGGAAAGA GGACCCTGCT TTGCCCACAC AGGTTCCTCT GCAGTACAAT 
GAGCTGAAGC TAGCCCTGGA AAAGGAGAAA GCCCGATGCG CGGAGCTGGA GGAGGCCCTT 
CAGAAGACCC GCATCGAACT CCGCTCTGCG CGGGAGGAAG CTGCCCACCG CAAAGCCACG 
GACCACCCGC ACCCGTCTAC GCCAGCCACT GCGAGGCAGC AGATCGCCAT GTCTGCCATT 
GTGCGGTCGC CCGAGCACCA GCCCAGTGCC ATGAGCCTGC TTGCCCCACC ATCCAGCCGC 
AGAAAGGAGT CGTCAACTCC AGAGGAATTC AGCCGGCGTC TGAAAGAGCG CATGCACCAC 
AACATCCCTC ACCGGTTTAA TGTGGGCCTG AACATGAGAG CCACCAAGTG CGCCGTGTGT 
CTGGATACTG TGCACTTTGG ACGCCAGGCA TCCAAATGCC TCGAATGTCA AGTGATGTGT 
CACCCCAAAT GCTCCACCTG CTTGCCCGCT ACCTGTGGCC TGCCAGCTGA ATATGCCACA 
CACTTCACTG AGGCCTTCTG CCGGGACAAA ATGAACTCCC CGGGGCTCCA GAGCAAGGAG 
CCTGGCAGCA GCTTGCACCT GGAAGGGTGG ATGAAAGTGC CCAGAAATAA CAAACGGGGA 
CAGCAAGGCT GGGACAGGAA GTACATTGTT CTGGAGGGGT CAAAAGTCCT CATCTATGAC 
AATGAAGCCA GAGAAGCTGG ACAGAGGCCG GTGGAAGAAT TTGAGCTGTG CCTTCCTGAC 
GGGGACGTAT CTATTCATGG TGCCGTTGGT GCTTCAGAAC TTGCAAATAC CGCCAAAGCA 
GATGTCCCAT ACATCCTGAA GATGGAGTCT CACCCACACA CCACCTGCTG GCCTGGGAGA 
ACCCTCTACT TGCTAGCACC CAGCTTCCCC GACAAGCAGC GCTGGGTCAC CGCCTTAGAA 
TCTGTCGTCG CAGGTGGGAG AGTTTCTAGG GAAAAGGCCG AAGCCGATGC TGCACGTGAC 
CATACTTCGA GTGAGCATCA GCCAGTGTGG GTTGAGAAAT TACTTGGAAA CTCTCTGCTG 
AAACTGGAGG GCGATGACCG GCTTGACATG AACTGCACCC TGCCCTTCAG TGACCAGGTA 
GTGCTGGTGG GCACGGAGGA AGGCCTCTAC GCCCTGAATG TCTTGAAAAA CTCCTTAACC 
CACATCCCAG GGATTGGCGC AGTCTTCCAA ATTTACATCA TCAAGGACCT GGAGAAGCTG 
CTCATGATAG CAGGGGAAGA GCGGGCTCTG TGCCTGGTGG ACGTGAAGAA GGTGAAGCAG 
TCCCTGGCGC AGTCACACCT GCCTGCCCAG CCCGACGTCT CCCCCAACAT ATTCGAAGCC 
GTCAAAGGCT GCCACTTGTT CGCTGCTGGC AAGATCGAGA ACAGCCTGTG CATCTGCGCC 
GCTATGCCAA GCAAAGTCGT CATCCTCCGC TACAATGACA ACCTCAGCAA GTACTGCATC 
CGCAAGGAGA TCGAGACCTC AGAGCCCTGC AGCTGTATCC ACTTCACCAA CTACAGCATC 
CTCATTGGGA CCAACAAATT CTATGAGATC GACATGAAGC AGTACACGCT TGACGAGTTC 
CTGGACAAGA ACGACCATTC CTTGGCACCT GCTGTGTTCG CCTCCTCGTC CAACAGCTTC 
CCTGTCTCCA TTGTGCAGGC GAACAGCGCC GGGCAGCGAG AAGAATACCT GCTGTGCTTC 
CACGAATTTG GGGTGTTCGT GGATTCTTAC GGAAGACGTA GCCGCACAGA TGATCTCAAG 
TGGAGTCGCT TACCTCTGGC CTTCGCCTAC AGAGAACCTT ATCTGTTTGT GACTCACTTC 
AACTCCCTGG AAGTCATTGA GATCCAGGCC AGATCCTCAC TGGGGAGCCC TGCCCGAGCA 
TATCTGGAAA TTCCAAACCC TCGCTACCTG GGCCCCGCGA TTTCCTCCGG AGCGATTTAC 
CTGGCCTCCT CATACCAGGA CAAGTTAAGG GTCATATGCT GCAAAGGAAA CCTCGTGAAG 
GAGTCAGGCA CTGAGCAGCA CCGGGTGCCC TCCACCTCCC GCAGCAGCCC CAACAAGCGA 
GGCCCACCAA CATACAACGA GCACATCACC AAACGCGTGG CCTCCAGCCC GGCGCCACCG 
GAAGGCCCCA GCCACCCCCG AGAGCCAAGC ACACCGCACC GCTACCGAGA CAGAGAGGGT 
CGGACAGAGC TGCGCAGGGA CAAGTCTCCA GGCCGCCCTC TGGAGCGGGA GAAGTCCCCA 
GGCCGAATGC TCAGCACTAG GAGAGAGCGG TCCCCAGGGA GACTGTTTGA AGACAGCAGC 
AGGGGCCGGC TGCCTGCAGG AGCAGTGAGG ACCCCACTGT CCCAGGTTAA CAAGGTCTGG 
GACCAGTCTT CAGTATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006530212.1
CDS	1419..6416
Translation	MLLGEEAMMEQEMTRLHRRVSEVEAVLSQKEVELKASETQRSLLEQDLATYITECSSLKRSLEQARMEVSQEDDKALQLLHDIREQSRKLQEIKEQEYQAQVEEMRLMMNQLEEDLVSARRRSDLYESELRESRLAAEEFKRKANECQHKLMKVVSHPPRGDSGGTALDDLHKMQGHAGLTSAKDQGKPEVGEYSKLEKINAEQQLKIQELQEKLEKAVKASTEATELLQNIRQAKERAERELEKLHNREDSSEGIKKKLVEAEERRHSLENKVKRLETMERRENRLKDDIQTKSEQIQQMADKILELEEKHREAQVSAQHLEVHLKQKEQHYEEKIKVLDNQIKKDLADKESLENMMQRHEEEAHEKGKILSEQKAMINAMDSKIRSLEQRIVELSEANKLAANSSLFTQRNMKAQEEMISELRQQKFYLETQAGKLEAQNRKLEEQLEKISHQDHSDKSRLLELETRLREVSLEHEEQKLELKRQLTELQLSLQERESQLTALQAARAALESQLRQAKTELEETTAEAEEEIQALTAHRDEIQRKFDALRNSCTVITDLEEQLNQLTEDNAELNNQNFYLSKQLDEASGANDEIVQLRSEVDHLRREITEREMQLTSQKQAQLSAPDLQTMEALKTTCTMLEEQVLDLEALNDELLEKERQWEAWRSVLGDEKSQFECRVRELQRMLDTEKQSRARADQRITESRQVVELAVKEHKAEILALQQALKEQKLKAESLSDKLNDLEKKHAMLEMNARSLQQKLETERELKQRLLEEQAKLQQQMDLQKNHIFRLTQGLQEALDRADLLKTERSDLEYQLENIQVLYSHEKVKMEGTISQQTKLIDFLQAKMDQPAKKKKGLFSRRKEDPALPTQVPLQYNELKLALEKEKARCAELEEALQKTRIELRSAREEAAHRKATDHPHPSTPATARQQIAMSAIVRSPEHQPSAMSLLAPPSSRRKESSTPEEFSRRLKERMHHNIPHRFNVGLNMRATKCAVCLDTVHFGRQASKCLECQVMCHPKCSTCLPATCGLPAEYATHFTEAFCRDKMNSPGLQSKEPGSSLHLEGWMKVPRNNKRGQQGWDRKYIVLEGSKVLIYDNEAREAGQRPVEEFELCLPDGDVSIHGAVGASELANTAKADVPYILKMESHPHTTCWPGRTLYLLAPSFPDKQRWVTALESVVAGGRVSREKAEADAARDHTSSEHQPVWVEKLLGNSLLKLEGDDRLDMNCTLPFSDQVVLVGTEEGLYALNVLKNSLTHIPGIGAVFQIYIIKDLEKLLMIAGEERALCLVDVKKVKQSLAQSHLPAQPDVSPNIFEAVKGCHLFAAGKIENSLCICAAMPSKVVILRYNDNLSKYCIRKEIETSEPCSCIHFTNYSILIGTNKFYEIDMKQYTLDEFLDKNDHSLAPAVFASSSNSFPVSIVQANSAGQREEYLLCFHEFGVFVDSYGRRSRTDDLKWSRLPLAFAYREPYLFVTHFNSLEVIEIQARSSLGSPARAYLEIPNPRYLGPAISSGAIYLASSYQDKLRVICCKGNLVKESGTEQHRVPSTSRSSPNKRGPPTYNEHITKRVASSPAPPEGPSHPREPSTPHRYRDREGRTELRRDKSPGRPLEREKSPGRMLSTRRERSPGRLFEDSSRGRLPAGAVRTPLSQVNKVWDQSSV

Target ORF information:

RefSeq Version	XM_006530149.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus citron (Cit), transcript variant X21, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006530149.3

ORF Insert Sequence:

ATGCTCCTGG GCGAAGAAGC AATGATGGAG CAGGAAATGA CCCGGTTACA TCGCAGAGTG 
TCAGAGGTGG AGGCTGTGCT TAGTCAGAAG GAGGTGGAGC TGAAGGCCTC TGAGACTCAG 
AGATCCCTCC TGGAGCAGGA CCTTGCTACC TACATCACAG AATGCAGTAG CTTAAAGCGA 
AGTTTGGAGC AAGCGCGGAT GGAGGTGTCC CAGGAGGATG ACAAAGCTCT GCAGCTTCTC 
CACGACATCC GAGAGCAGAG CCGGAAGCTC CAGGAGATCA AGGAGCAGGA GTACCAGGCT 
CAGGTGGAGG AGATGAGGCT GATGATGAAT CAGCTGGAAG AAGACCTGGT GTCAGCCCGC 
AGACGCAGCG ATCTCTACGA GTCTGAGCTG AGGGAGTCTC GGCTTGCCGC CGAGGAATTC 
AAGCGGAAGG CAAACGAATG TCAGCACAAA CTGATGAAGG TAGTCAGCCA CCCTCCAAGA 
GGGGACTCCG GGGGCACGGC CCTGGATGAT CTTCACAAGA TGCAAGGCCA CGCTGGCTTA 
ACGAGTGCTA AGGACCAAGG GAAGCCTGAA GTGGGAGAAT ATTCCAAACT GGAGAAGATC 
AATGCTGAGC AGCAGCTGAA GATCCAGGAG CTCCAGGAGA AGCTGGAGAA GGCTGTAAAA 
GCCAGCACAG AGGCCACCGA GCTCCTGCAG AATATCCGCC AGGCAAAGGA GCGAGCAGAG 
CGGGAGCTAG AGAAGCTACA CAACCGGGAA GACTCCTCCG AGGGCATCAA AAAGAAGCTG 
GTGGAAGCCG AGGAACGCCG CCACTCCCTG GAGAACAAGG TAAAGAGACT AGAGACCATG 
GAGCGTAGAG AGAACAGACT GAAGGATGAC ATCCAGACAA AGTCCGAACA GATCCAGCAG 
ATGGCTGATA AAATTCTGGA GCTGGAGGAG AAACATCGGG AGGCTCAGGT CTCAGCTCAA 
CATCTAGAAG TACACTTGAA ACAGAAAGAA CAGCACTACG AGGAAAAGAT CAAGGTATTG 
GACAATCAGA TAAAGAAGGA CCTGGCCGAC AAGGAGAGCC TGGAGAACAT GATGCAGAGA 
CACGAGGAGG AGGCCCACGA GAAGGGCAAG ATCCTCAGCG AGCAGAAGGC GATGATCAAC 
GCGATGGATT CCAAGATCCG ATCCCTGGAG CAGAGGATCG TGGAGCTGTC GGAAGCCAAC 
AAGCTTGCGG CAAACAGCAG TCTCTTCACC CAGAGGAACA TGAAGGCCCA GGAAGAGATG 
ATCTCAGAAC TCAGGCAGCA GAAATTTTAC CTGGAGACGC AGGCCGGAAA GCTGGAGGCC 
CAGAACCGAA AGCTGGAAGA GCAACTGGAG AAGATCAGCC ACCAAGATCA CAGTGACAAG 
AGTCGGCTGC TGGAGCTGGA AACAAGGCTG AGGGAGGTCA GCCTGGAGCA CGAGGAGCAG 
AAGCTGGAGC TGAAGCGGCA GCTCACGGAG CTGCAGCTGT CCCTGCAGGA GCGCGAGTCC 
CAGCTGACGG CCCTGCAGGC CGCCCGGGCA GCGCTGGAGA GCCAGCTCCG CCAGGCGAAG 
ACAGAGCTGG AGGAGACAAC CGCGGAAGCG GAGGAGGAGA TCCAGGCGCT CACGGCACAT 
CGAGATGAGA TCCAGCGCAA ATTCGATGCC CTTCGCAACA GCTGCACGGT CATCACCGAC 
CTGGAGGAGC AGCTGAACCA GCTCACCGAG GACAACGCCG AGCTCAACAA CCAAAACTTC 
TACCTGTCCA AACAACTCGA TGAGGCTTCC GGGGCCAATG ACGAGATTGT GCAGCTGCGG 
AGTGAGGTGG ACCATCTCCG CCGTGAGATC ACGGAGCGGG AGATGCAGCT CACCAGCCAG 
AAGCAAGCAC AACTCAGTGC TCCTGATTTG CAGACGATGG AGGCTCTGAA GACGACATGC 
ACCATGCTGG AGGAGCAGGT CCTGGACCTG GAGGCCCTGA ACGACGAGCT GCTGGAGAAG 
GAGCGCCAGT GGGAGGCCTG GCGGAGCGTC CTCGGCGACG AGAAGTCCCA GTTCGAGTGT 
CGAGTTCGAG AGCTACAGAG GATGCTGGAC ACCGAGAAGC AGAGCAGGGC TAGGGCCGAT 
CAGCGGATCA CCGAGTCGCG CCAGGTGGTG GAGTTGGCGG TGAAGGAACA CAAGGCCGAG 
ATTCTTGCTC TGCAGCAGGC TCTCAAGGAG CAGAAGCTCA AAGCCGAGAG CCTGTCGGAC 
AAGCTCAACG ACCTGGAGAA GAAGCACGCC ATGCTGGAGA TGAACGCCCG GAGCTTACAG 
CAGAAACTAG AGACAGAGCG GGAGCTCAAA CAGAGGCTTC TGGAGGAGCA AGCCAAATTG 
CAGCAGCAGA TGGACCTGCA GAAGAACCAC ATCTTCAGAC TGACGCAAGG GCTGCAGGAG 
GCGCTGGACC GGGCGGATCT GCTGAAGACA GAAAGGAGCG ACCTGGAGTA CCAGCTGGAA 
AACATTCAGG TTCTCTACTC TCACGAGAAA GTGAAAATGG AAGGCACAAT CTCTCAGCAA 
ACCAAACTCA TTGATTTCCT GCAAGCCAAA ATGGACCAGC CTGCTAAAAA GAAAAAGGGT 
TTATTTAGTC GACGGAAAGA GGACCCTGCT TTGCCCACAC AGGTTCCTCT GCAGTACAAT 
GAGCTGAAGC TAGCCCTGGA AAAGGAGAAA GCCCGATGCG CGGAGCTGGA GGAGGCCCTT 
CAGAAGACCC GCATCGAACT CCGCTCTGCG CGGGAGGAAG CTGCCCACCG CAAAGCCACG 
GACCACCCGC ACCCGTCTAC GCCAGCCACT GCGAGGCAGC AGATCGCCAT GTCTGCCATT 
GTGCGGTCGC CCGAGCACCA GCCCAGTGCC ATGAGCCTGC TTGCCCCACC ATCCAGCCGC 
AGAAAGGAGT CGTCAACTCC AGAGGAATTC AGCCGGCGTC TGAAAGAGCG CATGCACCAC 
AACATCCCTC ACCGGTTTAA TGTGGGCCTG AACATGAGAG CCACCAAGTG CGCCGTGTGT 
CTGGATACTG TGCACTTTGG ACGCCAGGCA TCCAAATGCC TCGAATGTCA AGTGATGTGT 
CACCCCAAAT GCTCCACCTG CTTGCCCGCT ACCTGTGGCC TGCCAGCTGA ATATGCCACA 
CACTTCACTG AGGCCTTCTG CCGGGACAAA ATGAACTCCC CGGGGCTCCA GAGCAAGGAG 
CCTGGCAGCA GCTTGCACCT GGAAGGGTGG ATGAAAGTGC CCAGAAATAA CAAACGGGGA 
CAGCAAGGCT GGGACAGGAA GTACATTGTT CTGGAGGGGT CAAAAGTCCT CATCTATGAC 
AATGAAGCCA GAGAAGCTGG ACAGAGGCCG GTGGAAGAAT TTGAGCTGTG CCTTCCTGAC 
GGGGACGTAT CTATTCATGG TGCCGTTGGT GCTTCAGAAC TTGCAAATAC CGCCAAAGCA 
GATGTCCCAT ACATCCTGAA GATGGAGTCT CACCCACACA CCACCTGCTG GCCTGGGAGA 
ACCCTCTACT TGCTAGCACC CAGCTTCCCC GACAAGCAGC GCTGGGTCAC CGCCTTAGAA 
TCTGTCGTCG CAGGTGGGAG AGTTTCTAGG GAAAAGGCCG AAGCCGATGC TGCACGTGAC 
CATACTTCGA GTGAGCATCA GCCAGTGTGG GTTGAGAAAT TACTTGGAAA CTCTCTGCTG 
AAACTGGAGG GCGATGACCG GCTTGACATG AACTGCACCC TGCCCTTCAG TGACCAGGTA 
GTGCTGGTGG GCACGGAGGA AGGCCTCTAC GCCCTGAATG TCTTGAAAAA CTCCTTAACC 
CACATCCCAG GGATTGGCGC AGTCTTCCAA ATTTACATCA TCAAGGACCT GGAGAAGCTG 
CTCATGATAG CAGGGGAAGA GCGGGCTCTG TGCCTGGTGG ACGTGAAGAA GGTGAAGCAG 
TCCCTGGCGC AGTCACACCT GCCTGCCCAG CCCGACGTCT CCCCCAACAT ATTCGAAGCC 
GTCAAAGGCT GCCACTTGTT CGCTGCTGGC AAGATCGAGA ACAGCCTGTG CATCTGCGCC 
GCTATGCCAA GCAAAGTCGT CATCCTCCGC TACAATGACA ACCTCAGCAA GTACTGCATC 
CGCAAGGAGA TCGAGACCTC AGAGCCCTGC AGCTGTATCC ACTTCACCAA CTACAGCATC 
CTCATTGGGA CCAACAAATT CTATGAGATC GACATGAAGC AGTACACGCT TGACGAGTTC 
CTGGACAAGA ACGACCATTC CTTGGCACCT GCTGTGTTCG CCTCCTCGTC CAACAGCTTC 
CCTGTCTCCA TTGTGCAGGC GAACAGCGCC GGGCAGCGAG AAGAATACCT GCTGTGCTTC 
CACGAATTTG GGGTGTTCGT GGATTCTTAC GGAAGACGTA GCCGCACAGA TGATCTCAAG 
TGGAGTCGCT TACCTCTGGC CTTCGCCTAC AGAGAACCTT ATCTGTTTGT GACTCACTTC 
AACTCCCTGG AAGTCATTGA GATCCAGGCC AGATCCTCAC TGGGGAGCCC TGCCCGAGCA 
TATCTGGAAA TTCCAAACCC TCGCTACCTG GGCCCCGCGA TTTCCTCCGG AGCGATTTAC 
CTGGCCTCCT CATACCAGGA CAAGTTAAGG GTCATATGCT GCAAAGGAAA CCTCGTGAAG 
GAGTCAGGCA CTGAGCAGCA CCGGGTGCCC TCCACCTCCC GCAGCAGCCC CAACAAGCGA 
GGCCCACCAA CATACAACGA GCACATCACC AAACGCGTGG CCTCCAGCCC GGCGCCACCG 
GAAGGCCCCA GCCACCCCCG AGAGCCAAGC ACACCGCACC GCTACCGAGA CAGAGAGGGT 
CGGACAGAGC TGCGCAGGGA CAAGTCTCCA GGCCGCCCTC TGGAGCGGGA GAAGTCCCCA 
GGCCGAATGC TCAGCACTAG GAGAGAGCGG TCCCCAGGGA GACTGTTTGA AGACAGCAGC 
AGGGGCCGGC TGCCTGCAGG AGCAGTGAGG ACCCCACTGT CCCAGGTTAA CAAGGTCTGG 
GACCAGTCTT CAGTATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cit ( XM_006530150.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006530213.1 Mus musculus citron (Cit), transcript variant X22, mRNA.

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CloneID	OMu35789
Clone ID Related Accession (Same CDS sequence)	XM_006530150.4 , XM_006530150.3
Accession Version	XM_006530150.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4974bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	citron Rho-interacting kinase isoform X18
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000071.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006530150.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGCAGG AAATGACCCG GTTACATCGC AGAGTGTCAG AGGTGGAGGC TGTGCTTAGT 
CAGAAGGAGG TGGAGCTGAA GGCCTCTGAG ACTCAGAGAT CCCTCCTGGA GCAGGACCTT 
GCTACCTACA TCACAGAATG CAGTAGCTTA AAGCGAAGTT TGGAGCAAGC GCGGATGGAG 
GTGTCCCAGG AGGATGACAA AGCTCTGCAG CTTCTCCACG ACATCCGAGA GCAGAGCCGG 
AAGCTCCAGG AGATCAAGGA GCAGGAGTAC CAGGCTCAGG TGGAGGAGAT GAGGCTGATG 
ATGAATCAGC TGGAAGAAGA CCTGGTGTCA GCCCGCAGAC GCAGCGATCT CTACGAGTCT 
GAGCTGAGGG AGTCTCGGCT TGCCGCCGAG GAATTCAAGC GGAAGGCAAA CGAATGTCAG 
CACAAACTGA TGAAGGTAGT CAGCCACCCT CCAAGAGGGG ACTCCGGGGG CACGGCCCTG 
GATGATCTTC ACAAGATGCA AGGCCACGCT GGCTTAACGA GTGCTAAGGA CCAAGGGAAG 
CCTGAAGTGG GAGAATATTC CAAACTGGAG AAGATCAATG CTGAGCAGCA GCTGAAGATC 
CAGGAGCTCC AGGAGAAGCT GGAGAAGGCT GTAAAAGCCA GCACAGAGGC CACCGAGCTC 
CTGCAGAATA TCCGCCAGGC AAAGGAGCGA GCAGAGCGGG AGCTAGAGAA GCTACACAAC 
CGGGAAGACT CCTCCGAGGG CATCAAAAAG AAGCTGGTGG AAGCCGAGGA ACGCCGCCAC 
TCCCTGGAGA ACAAGGTAAA GAGACTAGAG ACCATGGAGC GTAGAGAGAA CAGACTGAAG 
GATGACATCC AGACAAAGTC CGAACAGATC CAGCAGATGG CTGATAAAAT TCTGGAGCTG 
GAGGAGAAAC ATCGGGAGGC TCAGGTCTCA GCTCAACATC TAGAAGTACA CTTGAAACAG 
AAAGAACAGC ACTACGAGGA AAAGATCAAG GTATTGGACA ATCAGATAAA GAAGGACCTG 
GCCGACAAGG AGAGCCTGGA GAACATGATG CAGAGACACG AGGAGGAGGC CCACGAGAAG 
GGCAAGATCC TCAGCGAGCA GAAGGCGATG ATCAACGCGA TGGATTCCAA GATCCGATCC 
CTGGAGCAGA GGATCGTGGA GCTGTCGGAA GCCAACAAGC TTGCGGCAAA CAGCAGTCTC 
TTCACCCAGA GGAACATGAA GGCCCAGGAA GAGATGATCT CAGAACTCAG GCAGCAGAAA 
TTTTACCTGG AGACGCAGGC CGGAAAGCTG GAGGCCCAGA ACCGAAAGCT GGAAGAGCAA 
CTGGAGAAGA TCAGCCACCA AGATCACAGT GACAAGAGTC GGCTGCTGGA GCTGGAAACA 
AGGCTGAGGG AGGTCAGCCT GGAGCACGAG GAGCAGAAGC TGGAGCTGAA GCGGCAGCTC 
ACGGAGCTGC AGCTGTCCCT GCAGGAGCGC GAGTCCCAGC TGACGGCCCT GCAGGCCGCC 
CGGGCAGCGC TGGAGAGCCA GCTCCGCCAG GCGAAGACAG AGCTGGAGGA GACAACCGCG 
GAAGCGGAGG AGGAGATCCA GGCGCTCACG GCACATCGAG ATGAGATCCA GCGCAAATTC 
GATGCCCTTC GCAACAGCTG CACGGTCATC ACCGACCTGG AGGAGCAGCT GAACCAGCTC 
ACCGAGGACA ACGCCGAGCT CAACAACCAA AACTTCTACC TGTCCAAACA ACTCGATGAG 
GCTTCCGGGG CCAATGACGA GATTGTGCAG CTGCGGAGTG AGGTGGACCA TCTCCGCCGT 
GAGATCACGG AGCGGGAGAT GCAGCTCACC AGCCAGAAGC AAGCACAACT CAGTGCTCCT 
GATTTGCAGA CGATGGAGGC TCTGAAGACG ACATGCACCA TGCTGGAGGA GCAGGTCCTG 
GACCTGGAGG CCCTGAACGA CGAGCTGCTG GAGAAGGAGC GCCAGTGGGA GGCCTGGCGG 
AGCGTCCTCG GCGACGAGAA GTCCCAGTTC GAGTGTCGAG TTCGAGAGCT ACAGAGGATG 
CTGGACACCG AGAAGCAGAG CAGGGCTAGG GCCGATCAGC GGATCACCGA GTCGCGCCAG 
GTGGTGGAGT TGGCGGTGAA GGAACACAAG GCCGAGATTC TTGCTCTGCA GCAGGCTCTC 
AAGGAGCAGA AGCTCAAAGC CGAGAGCCTG TCGGACAAGC TCAACGACCT GGAGAAGAAG 
CACGCCATGC TGGAGATGAA CGCCCGGAGC TTACAGCAGA AACTAGAGAC AGAGCGGGAG 
CTCAAACAGA GGCTTCTGGA GGAGCAAGCC AAATTGCAGC AGCAGATGGA CCTGCAGAAG 
AACCACATCT TCAGACTGAC GCAAGGGCTG CAGGAGGCGC TGGACCGGGC GGATCTGCTG 
AAGACAGAAA GGAGCGACCT GGAGTACCAG CTGGAAAACA TTCAGGTTCT CTACTCTCAC 
GAGAAAGTGA AAATGGAAGG CACAATCTCT CAGCAAACCA AACTCATTGA TTTCCTGCAA 
GCCAAAATGG ACCAGCCTGC TAAAAAGAAA AAGGGTTTAT TTAGTCGACG GAAAGAGGAC 
CCTGCTTTGC CCACACAGGT TCCTCTGCAG TACAATGAGC TGAAGCTAGC CCTGGAAAAG 
GAGAAAGCCC GATGCGCGGA GCTGGAGGAG GCCCTTCAGA AGACCCGCAT CGAACTCCGC 
TCTGCGCGGG AGGAAGCTGC CCACCGCAAA GCCACGGACC ACCCGCACCC GTCTACGCCA 
GCCACTGCGA GGCAGCAGAT CGCCATGTCT GCCATTGTGC GGTCGCCCGA GCACCAGCCC 
AGTGCCATGA GCCTGCTTGC CCCACCATCC AGCCGCAGAA AGGAGTCGTC AACTCCAGAG 
GAATTCAGCC GGCGTCTGAA AGAGCGCATG CACCACAACA TCCCTCACCG GTTTAATGTG 
GGCCTGAACA TGAGAGCCAC CAAGTGCGCC GTGTGTCTGG ATACTGTGCA CTTTGGACGC 
CAGGCATCCA AATGCCTCGA ATGTCAAGTG ATGTGTCACC CCAAATGCTC CACCTGCTTG 
CCCGCTACCT GTGGCCTGCC AGCTGAATAT GCCACACACT TCACTGAGGC CTTCTGCCGG 
GACAAAATGA ACTCCCCGGG GCTCCAGAGC AAGGAGCCTG GCAGCAGCTT GCACCTGGAA 
GGGTGGATGA AAGTGCCCAG AAATAACAAA CGGGGACAGC AAGGCTGGGA CAGGAAGTAC 
ATTGTTCTGG AGGGGTCAAA AGTCCTCATC TATGACAATG AAGCCAGAGA AGCTGGACAG 
AGGCCGGTGG AAGAATTTGA GCTGTGCCTT CCTGACGGGG ACGTATCTAT TCATGGTGCC 
GTTGGTGCTT CAGAACTTGC AAATACCGCC AAAGCAGATG TCCCATACAT CCTGAAGATG 
GAGTCTCACC CACACACCAC CTGCTGGCCT GGGAGAACCC TCTACTTGCT AGCACCCAGC 
TTCCCCGACA AGCAGCGCTG GGTCACCGCC TTAGAATCTG TCGTCGCAGG TGGGAGAGTT 
TCTAGGGAAA AGGCCGAAGC CGATGCTGCA CGTGACCATA CTTCGAGTGA GCATCAGCCA 
GTGTGGGTTG AGAAATTACT TGGAAACTCT CTGCTGAAAC TGGAGGGCGA TGACCGGCTT 
GACATGAACT GCACCCTGCC CTTCAGTGAC CAGGTAGTGC TGGTGGGCAC GGAGGAAGGC 
CTCTACGCCC TGAATGTCTT GAAAAACTCC TTAACCCACA TCCCAGGGAT TGGCGCAGTC 
TTCCAAATTT ACATCATCAA GGACCTGGAG AAGCTGCTCA TGATAGCAGG GGAAGAGCGG 
GCTCTGTGCC TGGTGGACGT GAAGAAGGTG AAGCAGTCCC TGGCGCAGTC ACACCTGCCT 
GCCCAGCCCG ACGTCTCCCC CAACATATTC GAAGCCGTCA AAGGCTGCCA CTTGTTCGCT 
GCTGGCAAGA TCGAGAACAG CCTGTGCATC TGCGCCGCTA TGCCAAGCAA AGTCGTCATC 
CTCCGCTACA ATGACAACCT CAGCAAGTAC TGCATCCGCA AGGAGATCGA GACCTCAGAG 
CCCTGCAGCT GTATCCACTT CACCAACTAC AGCATCCTCA TTGGGACCAA CAAATTCTAT 
GAGATCGACA TGAAGCAGTA CACGCTTGAC GAGTTCCTGG ACAAGAACGA CCATTCCTTG 
GCACCTGCTG TGTTCGCCTC CTCGTCCAAC AGCTTCCCTG TCTCCATTGT GCAGGCGAAC 
AGCGCCGGGC AGCGAGAAGA ATACCTGCTG TGCTTCCACG AATTTGGGGT GTTCGTGGAT 
TCTTACGGAA GACGTAGCCG CACAGATGAT CTCAAGTGGA GTCGCTTACC TCTGGCCTTC 
GCCTACAGAG AACCTTATCT GTTTGTGACT CACTTCAACT CCCTGGAAGT CATTGAGATC 
CAGGCCAGAT CCTCACTGGG GAGCCCTGCC CGAGCATATC TGGAAATTCC AAACCCTCGC 
TACCTGGGCC CCGCGATTTC CTCCGGAGCG ATTTACCTGG CCTCCTCATA CCAGGACAAG 
TTAAGGGTCA TATGCTGCAA AGGAAACCTC GTGAAGGAGT CAGGCACTGA GCAGCACCGG 
GTGCCCTCCA CCTCCCGCAG CAGCCCCAAC AAGCGAGGCC CACCAACATA CAACGAGCAC 
ATCACCAAAC GCGTGGCCTC CAGCCCGGCG CCACCGGAAG GCCCCAGCCA CCCCCGAGAG 
CCAAGCACAC CGCACCGCTA CCGAGACAGA GAGGGTCGGA CAGAGCTGCG CAGGGACAAG 
TCTCCAGGCC GCCCTCTGGA GCGGGAGAAG TCCCCAGGCC GAATGCTCAG CACTAGGAGA 
GAGCGGTCCC CAGGGAGACT GTTTGAAGAC AGCAGCAGGG GCCGGCTGCC TGCAGGAGCA 
GTGAGGACCC CACTGTCCCA GGTTAACAAG GTCTGGGACC AGTCTTCAGT ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006530213.1
CDS	1475..6448
Translation	MEQEMTRLHRRVSEVEAVLSQKEVELKASETQRSLLEQDLATYITECSSLKRSLEQARMEVSQEDDKALQLLHDIREQSRKLQEIKEQEYQAQVEEMRLMMNQLEEDLVSARRRSDLYESELRESRLAAEEFKRKANECQHKLMKVVSHPPRGDSGGTALDDLHKMQGHAGLTSAKDQGKPEVGEYSKLEKINAEQQLKIQELQEKLEKAVKASTEATELLQNIRQAKERAERELEKLHNREDSSEGIKKKLVEAEERRHSLENKVKRLETMERRENRLKDDIQTKSEQIQQMADKILELEEKHREAQVSAQHLEVHLKQKEQHYEEKIKVLDNQIKKDLADKESLENMMQRHEEEAHEKGKILSEQKAMINAMDSKIRSLEQRIVELSEANKLAANSSLFTQRNMKAQEEMISELRQQKFYLETQAGKLEAQNRKLEEQLEKISHQDHSDKSRLLELETRLREVSLEHEEQKLELKRQLTELQLSLQERESQLTALQAARAALESQLRQAKTELEETTAEAEEEIQALTAHRDEIQRKFDALRNSCTVITDLEEQLNQLTEDNAELNNQNFYLSKQLDEASGANDEIVQLRSEVDHLRREITEREMQLTSQKQAQLSAPDLQTMEALKTTCTMLEEQVLDLEALNDELLEKERQWEAWRSVLGDEKSQFECRVRELQRMLDTEKQSRARADQRITESRQVVELAVKEHKAEILALQQALKEQKLKAESLSDKLNDLEKKHAMLEMNARSLQQKLETERELKQRLLEEQAKLQQQMDLQKNHIFRLTQGLQEALDRADLLKTERSDLEYQLENIQVLYSHEKVKMEGTISQQTKLIDFLQAKMDQPAKKKKGLFSRRKEDPALPTQVPLQYNELKLALEKEKARCAELEEALQKTRIELRSAREEAAHRKATDHPHPSTPATARQQIAMSAIVRSPEHQPSAMSLLAPPSSRRKESSTPEEFSRRLKERMHHNIPHRFNVGLNMRATKCAVCLDTVHFGRQASKCLECQVMCHPKCSTCLPATCGLPAEYATHFTEAFCRDKMNSPGLQSKEPGSSLHLEGWMKVPRNNKRGQQGWDRKYIVLEGSKVLIYDNEAREAGQRPVEEFELCLPDGDVSIHGAVGASELANTAKADVPYILKMESHPHTTCWPGRTLYLLAPSFPDKQRWVTALESVVAGGRVSREKAEADAARDHTSSEHQPVWVEKLLGNSLLKLEGDDRLDMNCTLPFSDQVVLVGTEEGLYALNVLKNSLTHIPGIGAVFQIYIIKDLEKLLMIAGEERALCLVDVKKVKQSLAQSHLPAQPDVSPNIFEAVKGCHLFAAGKIENSLCICAAMPSKVVILRYNDNLSKYCIRKEIETSEPCSCIHFTNYSILIGTNKFYEIDMKQYTLDEFLDKNDHSLAPAVFASSSNSFPVSIVQANSAGQREEYLLCFHEFGVFVDSYGRRSRTDDLKWSRLPLAFAYREPYLFVTHFNSLEVIEIQARSSLGSPARAYLEIPNPRYLGPAISSGAIYLASSYQDKLRVICCKGNLVKESGTEQHRVPSTSRSSPNKRGPPTYNEHITKRVASSPAPPEGPSHPREPSTPHRYRDREGRTELRRDKSPGRPLEREKSPGRMLSTRRERSPGRLFEDSSRGRLPAGAVRTPLSQVNKVWDQSSV

Target ORF information:

RefSeq Version	XM_006530150.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus citron (Cit), transcript variant X22, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006530150.3

ORF Insert Sequence:

ATGGAGCAGG AAATGACCCG GTTACATCGC AGAGTGTCAG AGGTGGAGGC TGTGCTTAGT 
CAGAAGGAGG TGGAGCTGAA GGCCTCTGAG ACTCAGAGAT CCCTCCTGGA GCAGGACCTT 
GCTACCTACA TCACAGAATG CAGTAGCTTA AAGCGAAGTT TGGAGCAAGC GCGGATGGAG 
GTGTCCCAGG AGGATGACAA AGCTCTGCAG CTTCTCCACG ACATCCGAGA GCAGAGCCGG 
AAGCTCCAGG AGATCAAGGA GCAGGAGTAC CAGGCTCAGG TGGAGGAGAT GAGGCTGATG 
ATGAATCAGC TGGAAGAAGA CCTGGTGTCA GCCCGCAGAC GCAGCGATCT CTACGAGTCT 
GAGCTGAGGG AGTCTCGGCT TGCCGCCGAG GAATTCAAGC GGAAGGCAAA CGAATGTCAG 
CACAAACTGA TGAAGGTAGT CAGCCACCCT CCAAGAGGGG ACTCCGGGGG CACGGCCCTG 
GATGATCTTC ACAAGATGCA AGGCCACGCT GGCTTAACGA GTGCTAAGGA CCAAGGGAAG 
CCTGAAGTGG GAGAATATTC CAAACTGGAG AAGATCAATG CTGAGCAGCA GCTGAAGATC 
CAGGAGCTCC AGGAGAAGCT GGAGAAGGCT GTAAAAGCCA GCACAGAGGC CACCGAGCTC 
CTGCAGAATA TCCGCCAGGC AAAGGAGCGA GCAGAGCGGG AGCTAGAGAA GCTACACAAC 
CGGGAAGACT CCTCCGAGGG CATCAAAAAG AAGCTGGTGG AAGCCGAGGA ACGCCGCCAC 
TCCCTGGAGA ACAAGGTAAA GAGACTAGAG ACCATGGAGC GTAGAGAGAA CAGACTGAAG 
GATGACATCC AGACAAAGTC CGAACAGATC CAGCAGATGG CTGATAAAAT TCTGGAGCTG 
GAGGAGAAAC ATCGGGAGGC TCAGGTCTCA GCTCAACATC TAGAAGTACA CTTGAAACAG 
AAAGAACAGC ACTACGAGGA AAAGATCAAG GTATTGGACA ATCAGATAAA GAAGGACCTG 
GCCGACAAGG AGAGCCTGGA GAACATGATG CAGAGACACG AGGAGGAGGC CCACGAGAAG 
GGCAAGATCC TCAGCGAGCA GAAGGCGATG ATCAACGCGA TGGATTCCAA GATCCGATCC 
CTGGAGCAGA GGATCGTGGA GCTGTCGGAA GCCAACAAGC TTGCGGCAAA CAGCAGTCTC 
TTCACCCAGA GGAACATGAA GGCCCAGGAA GAGATGATCT CAGAACTCAG GCAGCAGAAA 
TTTTACCTGG AGACGCAGGC CGGAAAGCTG GAGGCCCAGA ACCGAAAGCT GGAAGAGCAA 
CTGGAGAAGA TCAGCCACCA AGATCACAGT GACAAGAGTC GGCTGCTGGA GCTGGAAACA 
AGGCTGAGGG AGGTCAGCCT GGAGCACGAG GAGCAGAAGC TGGAGCTGAA GCGGCAGCTC 
ACGGAGCTGC AGCTGTCCCT GCAGGAGCGC GAGTCCCAGC TGACGGCCCT GCAGGCCGCC 
CGGGCAGCGC TGGAGAGCCA GCTCCGCCAG GCGAAGACAG AGCTGGAGGA GACAACCGCG 
GAAGCGGAGG AGGAGATCCA GGCGCTCACG GCACATCGAG ATGAGATCCA GCGCAAATTC 
GATGCCCTTC GCAACAGCTG CACGGTCATC ACCGACCTGG AGGAGCAGCT GAACCAGCTC 
ACCGAGGACA ACGCCGAGCT CAACAACCAA AACTTCTACC TGTCCAAACA ACTCGATGAG 
GCTTCCGGGG CCAATGACGA GATTGTGCAG CTGCGGAGTG AGGTGGACCA TCTCCGCCGT 
GAGATCACGG AGCGGGAGAT GCAGCTCACC AGCCAGAAGC AAGCACAACT CAGTGCTCCT 
GATTTGCAGA CGATGGAGGC TCTGAAGACG ACATGCACCA TGCTGGAGGA GCAGGTCCTG 
GACCTGGAGG CCCTGAACGA CGAGCTGCTG GAGAAGGAGC GCCAGTGGGA GGCCTGGCGG 
AGCGTCCTCG GCGACGAGAA GTCCCAGTTC GAGTGTCGAG TTCGAGAGCT ACAGAGGATG 
CTGGACACCG AGAAGCAGAG CAGGGCTAGG GCCGATCAGC GGATCACCGA GTCGCGCCAG 
GTGGTGGAGT TGGCGGTGAA GGAACACAAG GCCGAGATTC TTGCTCTGCA GCAGGCTCTC 
AAGGAGCAGA AGCTCAAAGC CGAGAGCCTG TCGGACAAGC TCAACGACCT GGAGAAGAAG 
CACGCCATGC TGGAGATGAA CGCCCGGAGC TTACAGCAGA AACTAGAGAC AGAGCGGGAG 
CTCAAACAGA GGCTTCTGGA GGAGCAAGCC AAATTGCAGC AGCAGATGGA CCTGCAGAAG 
AACCACATCT TCAGACTGAC GCAAGGGCTG CAGGAGGCGC TGGACCGGGC GGATCTGCTG 
AAGACAGAAA GGAGCGACCT GGAGTACCAG CTGGAAAACA TTCAGGTTCT CTACTCTCAC 
GAGAAAGTGA AAATGGAAGG CACAATCTCT CAGCAAACCA AACTCATTGA TTTCCTGCAA 
GCCAAAATGG ACCAGCCTGC TAAAAAGAAA AAGGGTTTAT TTAGTCGACG GAAAGAGGAC 
CCTGCTTTGC CCACACAGGT TCCTCTGCAG TACAATGAGC TGAAGCTAGC CCTGGAAAAG 
GAGAAAGCCC GATGCGCGGA GCTGGAGGAG GCCCTTCAGA AGACCCGCAT CGAACTCCGC 
TCTGCGCGGG AGGAAGCTGC CCACCGCAAA GCCACGGACC ACCCGCACCC GTCTACGCCA 
GCCACTGCGA GGCAGCAGAT CGCCATGTCT GCCATTGTGC GGTCGCCCGA GCACCAGCCC 
AGTGCCATGA GCCTGCTTGC CCCACCATCC AGCCGCAGAA AGGAGTCGTC AACTCCAGAG 
GAATTCAGCC GGCGTCTGAA AGAGCGCATG CACCACAACA TCCCTCACCG GTTTAATGTG 
GGCCTGAACA TGAGAGCCAC CAAGTGCGCC GTGTGTCTGG ATACTGTGCA CTTTGGACGC 
CAGGCATCCA AATGCCTCGA ATGTCAAGTG ATGTGTCACC CCAAATGCTC CACCTGCTTG 
CCCGCTACCT GTGGCCTGCC AGCTGAATAT GCCACACACT TCACTGAGGC CTTCTGCCGG 
GACAAAATGA ACTCCCCGGG GCTCCAGAGC AAGGAGCCTG GCAGCAGCTT GCACCTGGAA 
GGGTGGATGA AAGTGCCCAG AAATAACAAA CGGGGACAGC AAGGCTGGGA CAGGAAGTAC 
ATTGTTCTGG AGGGGTCAAA AGTCCTCATC TATGACAATG AAGCCAGAGA AGCTGGACAG 
AGGCCGGTGG AAGAATTTGA GCTGTGCCTT CCTGACGGGG ACGTATCTAT TCATGGTGCC 
GTTGGTGCTT CAGAACTTGC AAATACCGCC AAAGCAGATG TCCCATACAT CCTGAAGATG 
GAGTCTCACC CACACACCAC CTGCTGGCCT GGGAGAACCC TCTACTTGCT AGCACCCAGC 
TTCCCCGACA AGCAGCGCTG GGTCACCGCC TTAGAATCTG TCGTCGCAGG TGGGAGAGTT 
TCTAGGGAAA AGGCCGAAGC CGATGCTGCA CGTGACCATA CTTCGAGTGA GCATCAGCCA 
GTGTGGGTTG AGAAATTACT TGGAAACTCT CTGCTGAAAC TGGAGGGCGA TGACCGGCTT 
GACATGAACT GCACCCTGCC CTTCAGTGAC CAGGTAGTGC TGGTGGGCAC GGAGGAAGGC 
CTCTACGCCC TGAATGTCTT GAAAAACTCC TTAACCCACA TCCCAGGGAT TGGCGCAGTC 
TTCCAAATTT ACATCATCAA GGACCTGGAG AAGCTGCTCA TGATAGCAGG GGAAGAGCGG 
GCTCTGTGCC TGGTGGACGT GAAGAAGGTG AAGCAGTCCC TGGCGCAGTC ACACCTGCCT 
GCCCAGCCCG ACGTCTCCCC CAACATATTC GAAGCCGTCA AAGGCTGCCA CTTGTTCGCT 
GCTGGCAAGA TCGAGAACAG CCTGTGCATC TGCGCCGCTA TGCCAAGCAA AGTCGTCATC 
CTCCGCTACA ATGACAACCT CAGCAAGTAC TGCATCCGCA AGGAGATCGA GACCTCAGAG 
CCCTGCAGCT GTATCCACTT CACCAACTAC AGCATCCTCA TTGGGACCAA CAAATTCTAT 
GAGATCGACA TGAAGCAGTA CACGCTTGAC GAGTTCCTGG ACAAGAACGA CCATTCCTTG 
GCACCTGCTG TGTTCGCCTC CTCGTCCAAC AGCTTCCCTG TCTCCATTGT GCAGGCGAAC 
AGCGCCGGGC AGCGAGAAGA ATACCTGCTG TGCTTCCACG AATTTGGGGT GTTCGTGGAT 
TCTTACGGAA GACGTAGCCG CACAGATGAT CTCAAGTGGA GTCGCTTACC TCTGGCCTTC 
GCCTACAGAG AACCTTATCT GTTTGTGACT CACTTCAACT CCCTGGAAGT CATTGAGATC 
CAGGCCAGAT CCTCACTGGG GAGCCCTGCC CGAGCATATC TGGAAATTCC AAACCCTCGC 
TACCTGGGCC CCGCGATTTC CTCCGGAGCG ATTTACCTGG CCTCCTCATA CCAGGACAAG 
TTAAGGGTCA TATGCTGCAA AGGAAACCTC GTGAAGGAGT CAGGCACTGA GCAGCACCGG 
GTGCCCTCCA CCTCCCGCAG CAGCCCCAAC AAGCGAGGCC CACCAACATA CAACGAGCAC 
ATCACCAAAC GCGTGGCCTC CAGCCCGGCG CCACCGGAAG GCCCCAGCCA CCCCCGAGAG 
CCAAGCACAC CGCACCGCTA CCGAGACAGA GAGGGTCGGA CAGAGCTGCG CAGGGACAAG 
TCTCCAGGCC GCCCTCTGGA GCGGGAGAAG TCCCCAGGCC GAATGCTCAG CACTAGGAGA 
GAGCGGTCCC CAGGGAGACT GTTTGAAGAC AGCAGCAGGG GCCGGCTGCC TGCAGGAGCA 
GTGAGGACCC CACTGTCCCA GGTTAACAAG GTCTGGGACC AGTCTTCAGT ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cit ( XM_006530151.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006530214.1 Mus musculus citron (Cit), transcript variant X30, mRNA.

Price & Availability↑

CloneID	OMu35790
Clone ID Related Accession (Same CDS sequence)	XM_006530151.3 , XM_006530151.4
Accession Version	XM_006530151.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4794bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	citron Rho-interacting kinase isoform X26
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000071.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006530151.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTCCTGG GCGAAGAAGC AATGATGGAG CAGGAAATGA CCCGGTTACA TCGCAGAGTG 
TCAGAGGTGG AGGCTGTGCT TAGTCAGAAG GAGGTGGAGC TGAAGGCCTC TGAGACTCAG 
AGATCCCTCC TGGAGCAGGA CCTTGCTACC TACATCACAG AATGCAGTAG CTTAAAGCGA 
AGTTTGGAGC AAGCGCGGAT GGAGGTGTCC CAGGAGGATG ACAAAGCTCT GCAGCTTCTC 
CACGACATCC GAGAGCAGAG CCGGAAGCTC CAGGAGATCA AGGAGCAGGA GTACCAGGCT 
CAGGTGGAGG AGATGAGGCT GATGATGAAT CAGCTGGAAG AAGACCTGGT GTCAGCCCGC 
AGACGCAGCG ATCTCTACGA GTCTGAGCTG AGGGAGTCTC GGCTTGCCGC CGAGGAATTC 
AAGCGGAAGG CAAACGAATG TCAGCACAAA CTGATGAAGG CTAAGGACCA AGGGAAGCCT 
GAAGTGGGAG AATATTCCAA ACTGGAGAAG ATCAATGCTG AGCAGCAGCT GAAGATCCAG 
GAGCTCCAGG AGAAGCTGGA GAAGGCTGTA AAAGCCAGCA CAGAGGCCAC CGAGCTCCTG 
CAGAATATCC GCCAGGCAAA GGAGCGAGCA GAGCGGGAGC TAGAGAAGCT ACACAACCGG 
GAAGACTCCT CCGAGGGCAT CAAAAAGAAG CTGGTGGAAG CCGAGGAACG CCGCCACTCC 
CTGGAGAACA AGGTAAAGAG ACTAGAGACC ATGGAGCGTA GAGAGAACAG ACTGAAGGAT 
GACATCCAGA CAAAGTCCGA ACAGATCCAG CAGATGGCTG ATAAAATTCT GGAGCTGGAG 
GAGAAACATC GGGAGGCTCA GGTCTCAGCT CAACATCTAG AAGTACACTT GAAACAGAAA 
GAACAGCACT ACGAGGAAAA GATCAAGGTA TTGGACAATC AGATAAAGAA GGACCTGGCC 
GACAAGGAGA GCCTGGAGAA CATGATGCAG AGACACGAGG AGGAGGCCCA CGAGAAGGGC 
AAGATCCTCA GCGAGCAGAA GGCGATGATC AACGCGATGG ATTCCAAGAT CCGATCCCTG 
GAGCAGAGGA TCGTGGAGCT GTCGGAAGCC AACAAGCTTG CGGCAAACAG CAGTCTCTTC 
ACCCAGAGGA ACATGAAGGC CCAGGAAGAG ATGATCTCAG AACTCAGGCA GCAGAAATTT 
TACCTGGAGA CGCAGGCCGG AAAGCTGGAG GCCCAGAACC GAAAGCTGGA AGAGCAACTG 
GAGAAGATCA GCCACCAAGA TCACAGTGAC AAGAGTCGGC TGCTGGAGCT GGAAACAAGG 
CTGAGGGAGG TCAGCCTGGA GCACGAGGAG CAGAAGCTGG AGCTGAAGCG GCAGCTCACG 
GAGCTGCAGC TGTCCCTGCA GGAGCGCGAG TCCCAGCTGA CGGCCCTGCA GGCCGCCCGG 
GCAGCGCTGG AGAGCCAGCT CCGCCAGGCG AAGACAGAGC TGGAGGAGAC AACCGCGGAA 
GCGGAGGAGG AGATCCAGGC GCTCACGGCA CATCGAGATG AGATCCAGCG CAAATTCGAT 
GCCCTTCGCA ACAGCTGCAC GGTCATCACC GACCTGGAGG AGCAGCTGAA CCAGCTCACC 
GAGGACAACG CCGAGCTCAA CAACCAAAAC TTCTACCTGT CCAAACAACT CGATGAGGCT 
TCCGGGGCCA ATGACGAGAT TGTGCAGCTG CGGAGTGAGG TGGACCATCT CCGCCGTGAG 
ATCACGGAGC GGGAGATGCA GCTCACCAGC CAGAAGCAAA CGATGGAGGC TCTGAAGACG 
ACATGCACCA TGCTGGAGGA GCAGGTCCTG GACCTGGAGG CCCTGAACGA CGAGCTGCTG 
GAGAAGGAGC GCCAGTGGGA GGCCTGGCGG AGCGTCCTCG GCGACGAGAA GTCCCAGTTC 
GAGTGTCGAG TTCGAGAGCT ACAGAGGATG CTGGACACCG AGAAGCAGAG CAGGGCTAGG 
GCCGATCAGC GGATCACCGA GTCGCGCCAG GTGGTGGAGT TGGCGGTGAA GGAACACAAG 
GCCGAGATTC TTGCTCTGCA GCAGGCTCTC AAGGAGCAGA AGCTCAAAGC CGAGAGCCTG 
TCGGACAAGC TCAACGACCT GGAGAAGAAG CACGCCATGC TGGAGATGAA CGCCCGGAGC 
TTACAGCAGA AACTAGAGAC AGAGCGGGAG CTCAAACAGA GGCTTCTGGA GGAGCAAGCC 
AAATTGCAGC AGCAGATGGA CCTGCAGAAG AACCACATCT TCAGACTGAC GCAAGGGCTG 
CAGGAGGCGC TGGACCGGGC GGATCTGCTG AAGACAGAAA GGAGCGACCT GGAGTACCAG 
CTGGAAAACA TTCAGGTTCT CTACTCTCAC GAGAAAGTGA AAATGGAAGG CACAATCTCT 
CAGCAAACCA AACTCATTGA TTTCCTGCAA GCCAAAATGG ACCAGCCTGC TAAAAAGAAA 
AAGGTTCCTC TGCAGTACAA TGAGCTGAAG CTAGCCCTGG AAAAGGAGAA AGCCCGATGC 
GCGGAGCTGG AGGAGGCCCT TCAGAAGACC CGCATCGAAC TCCGCTCTGC GCGGGAGGAA 
GCTGCCCACC GCAAAGCCAC GGACCACCCG CACCCGTCTA CGCCAGCCAC TGCGAGGCAG 
CAGATCGCCA TGTCTGCCAT TGTGCGGTCG CCCGAGCACC AGCCCAGTGC CATGAGCCTG 
CTTGCCCCAC CATCCAGCCG CAGAAAGGAG TCGTCAACTC CAGAGGAATT CAGCCGGCGT 
CTGAAAGAGC GCATGCACCA CAACATCCCT CACCGGTTTA ATGTGGGCCT GAACATGAGA 
GCCACCAAGT GCGCCGTGTG TCTGGATACT GTGCACTTTG GACGCCAGGC ATCCAAATGC 
CTCGAATGTC AAGTGATGTG TCACCCCAAA TGCTCCACCT GCTTGCCCGC TACCTGTGGC 
CTGCCAGCTG AATATGCCAC ACACTTCACT GAGGCCTTCT GCCGGGACAA AATGAACTCC 
CCGGGGCTCC AGAGCAAGGA GCCTGGCAGC AGCTTGCACC TGGAAGGGTG GATGAAAGTG 
CCCAGAAATA ACAAACGGGG ACAGCAAGGC TGGGACAGGA AGTACATTGT TCTGGAGGGG 
TCAAAAGTCC TCATCTATGA CAATGAAGCC AGAGAAGCTG GACAGAGGCC GGTGGAAGAA 
TTTGAGCTGT GCCTTCCTGA CGGGGACGTA TCTATTCATG GTGCCGTTGG TGCTTCAGAA 
CTTGCAAATA CCGCCAAAGC AGATGTCCCA TACATCCTGA AGATGGAGTC TCACCCACAC 
ACCACCTGCT GGCCTGGGAG AACCCTCTAC TTGCTAGCAC CCAGCTTCCC CGACAAGCAG 
CGCTGGGTCA CCGCCTTAGA ATCTGTCGTC GCAGGTGGGA GAGTTTCTAG GGAAAAGGCC 
GAAGCCGATG CTAAATTACT TGGAAACTCT CTGCTGAAAC TGGAGGGCGA TGACCGGCTT 
GACATGAACT GCACCCTGCC CTTCAGTGAC CAGGTAGTGC TGGTGGGCAC GGAGGAAGGC 
CTCTACGCCC TGAATGTCTT GAAAAACTCC TTAACCCACA TCCCAGGGAT TGGCGCAGTC 
TTCCAAATTT ACATCATCAA GGACCTGGAG AAGCTGCTCA TGATAGCAGG GGAAGAGCGG 
GCTCTGTGCC TGGTGGACGT GAAGAAGGTG AAGCAGTCCC TGGCGCAGTC ACACCTGCCT 
GCCCAGCCCG ACGTCTCCCC CAACATATTC GAAGCCGTCA AAGGCTGCCA CTTGTTCGCT 
GCTGGCAAGA TCGAGAACAG CCTGTGCATC TGCGCCGCTA TGCCAAGCAA AGTCGTCATC 
CTCCGCTACA ATGACAACCT CAGCAAGTAC TGCATCCGCA AGGAGATCGA GACCTCAGAG 
CCCTGCAGCT GTATCCACTT CACCAACTAC AGCATCCTCA TTGGGACCAA CAAATTCTAT 
GAGATCGACA TGAAGCAGTA CACGCTTGAC GAGTTCCTGG ACAAGAACGA CCATTCCTTG 
GCACCTGCTG TGTTCGCCTC CTCGTCCAAC AGCTTCCCTG TCTCCATTGT GCAGGCGAAC 
AGCGCCGGGC AGCGAGAAGA ATACCTGCTG TGCTTCCACG AATTTGGGGT GTTCGTGGAT 
TCTTACGGAA GACGTAGCCG CACAGATGAT CTCAAGTGGA GTCGCTTACC TCTGGCCTTC 
GCCTACAGAG AACCTTATCT GTTTGTGACT CACTTCAACT CCCTGGAAGT CATTGAGATC 
CAGGCCAGAT CCTCACTGGG GAGCCCTGCC CGAGCATATC TGGAAATTCC AAACCCTCGC 
TACCTGGGCC CCGCGATTTC CTCCGGAGCG ATTTACCTGG CCTCCTCATA CCAGGACAAG 
TTAAGGGTCA TATGCTGCAA AGGAAACCTC GTGAAGGAGT CAGGCACTGA GCAGCACCGG 
GTGCCCTCCA CCTCCCGCAG CAGCCCCAAC AAGCGAGGCC CACCAACATA CAACGAGCAC 
ATCACCAAAC GCGTGGCCTC CAGCCCGGCG CCACCGGAAG GCCCCAGCCA CCCCCGAGAG 
CCAAGCACAC CGCACCGCTA CCGAGACAGA GAGGGTCGGA CAGAGCTGCG CAGGGACAAG 
TCTCCAGGCC GCCCTCTGGA GCGGGAGAAG TCCCCAGGCC GAATGCTCAG CACTAGGAGA 
GAGCGGTCCC CAGGGAGACT GTTTGAAGAC AGCAGCAGGG GCCGGCTGCC TGCAGGAGCA 
GTGAGGACCC CACTGTCCCA GGTTAACAAG GTCTGGGACC AGTCTTCAGT ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006530214.1
CDS	1419..6212
Translation	MLLGEEAMMEQEMTRLHRRVSEVEAVLSQKEVELKASETQRSLLEQDLATYITECSSLKRSLEQARMEVSQEDDKALQLLHDIREQSRKLQEIKEQEYQAQVEEMRLMMNQLEEDLVSARRRSDLYESELRESRLAAEEFKRKANECQHKLMKAKDQGKPEVGEYSKLEKINAEQQLKIQELQEKLEKAVKASTEATELLQNIRQAKERAERELEKLHNREDSSEGIKKKLVEAEERRHSLENKVKRLETMERRENRLKDDIQTKSEQIQQMADKILELEEKHREAQVSAQHLEVHLKQKEQHYEEKIKVLDNQIKKDLADKESLENMMQRHEEEAHEKGKILSEQKAMINAMDSKIRSLEQRIVELSEANKLAANSSLFTQRNMKAQEEMISELRQQKFYLETQAGKLEAQNRKLEEQLEKISHQDHSDKSRLLELETRLREVSLEHEEQKLELKRQLTELQLSLQERESQLTALQAARAALESQLRQAKTELEETTAEAEEEIQALTAHRDEIQRKFDALRNSCTVITDLEEQLNQLTEDNAELNNQNFYLSKQLDEASGANDEIVQLRSEVDHLRREITEREMQLTSQKQTMEALKTTCTMLEEQVLDLEALNDELLEKERQWEAWRSVLGDEKSQFECRVRELQRMLDTEKQSRARADQRITESRQVVELAVKEHKAEILALQQALKEQKLKAESLSDKLNDLEKKHAMLEMNARSLQQKLETERELKQRLLEEQAKLQQQMDLQKNHIFRLTQGLQEALDRADLLKTERSDLEYQLENIQVLYSHEKVKMEGTISQQTKLIDFLQAKMDQPAKKKKVPLQYNELKLALEKEKARCAELEEALQKTRIELRSAREEAAHRKATDHPHPSTPATARQQIAMSAIVRSPEHQPSAMSLLAPPSSRRKESSTPEEFSRRLKERMHHNIPHRFNVGLNMRATKCAVCLDTVHFGRQASKCLECQVMCHPKCSTCLPATCGLPAEYATHFTEAFCRDKMNSPGLQSKEPGSSLHLEGWMKVPRNNKRGQQGWDRKYIVLEGSKVLIYDNEAREAGQRPVEEFELCLPDGDVSIHGAVGASELANTAKADVPYILKMESHPHTTCWPGRTLYLLAPSFPDKQRWVTALESVVAGGRVSREKAEADAKLLGNSLLKLEGDDRLDMNCTLPFSDQVVLVGTEEGLYALNVLKNSLTHIPGIGAVFQIYIIKDLEKLLMIAGEERALCLVDVKKVKQSLAQSHLPAQPDVSPNIFEAVKGCHLFAAGKIENSLCICAAMPSKVVILRYNDNLSKYCIRKEIETSEPCSCIHFTNYSILIGTNKFYEIDMKQYTLDEFLDKNDHSLAPAVFASSSNSFPVSIVQANSAGQREEYLLCFHEFGVFVDSYGRRSRTDDLKWSRLPLAFAYREPYLFVTHFNSLEVIEIQARSSLGSPARAYLEIPNPRYLGPAISSGAIYLASSYQDKLRVICCKGNLVKESGTEQHRVPSTSRSSPNKRGPPTYNEHITKRVASSPAPPEGPSHPREPSTPHRYRDREGRTELRRDKSPGRPLEREKSPGRMLSTRRERSPGRLFEDSSRGRLPAGAVRTPLSQVNKVWDQSSV

Target ORF information:

RefSeq Version	XM_006530151.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus citron (Cit), transcript variant X30, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006530151.3

ORF Insert Sequence:

ATGCTCCTGG GCGAAGAAGC AATGATGGAG CAGGAAATGA CCCGGTTACA TCGCAGAGTG 
TCAGAGGTGG AGGCTGTGCT TAGTCAGAAG GAGGTGGAGC TGAAGGCCTC TGAGACTCAG 
AGATCCCTCC TGGAGCAGGA CCTTGCTACC TACATCACAG AATGCAGTAG CTTAAAGCGA 
AGTTTGGAGC AAGCGCGGAT GGAGGTGTCC CAGGAGGATG ACAAAGCTCT GCAGCTTCTC 
CACGACATCC GAGAGCAGAG CCGGAAGCTC CAGGAGATCA AGGAGCAGGA GTACCAGGCT 
CAGGTGGAGG AGATGAGGCT GATGATGAAT CAGCTGGAAG AAGACCTGGT GTCAGCCCGC 
AGACGCAGCG ATCTCTACGA GTCTGAGCTG AGGGAGTCTC GGCTTGCCGC CGAGGAATTC 
AAGCGGAAGG CAAACGAATG TCAGCACAAA CTGATGAAGG CTAAGGACCA AGGGAAGCCT 
GAAGTGGGAG AATATTCCAA ACTGGAGAAG ATCAATGCTG AGCAGCAGCT GAAGATCCAG 
GAGCTCCAGG AGAAGCTGGA GAAGGCTGTA AAAGCCAGCA CAGAGGCCAC CGAGCTCCTG 
CAGAATATCC GCCAGGCAAA GGAGCGAGCA GAGCGGGAGC TAGAGAAGCT ACACAACCGG 
GAAGACTCCT CCGAGGGCAT CAAAAAGAAG CTGGTGGAAG CCGAGGAACG CCGCCACTCC 
CTGGAGAACA AGGTAAAGAG ACTAGAGACC ATGGAGCGTA GAGAGAACAG ACTGAAGGAT 
GACATCCAGA CAAAGTCCGA ACAGATCCAG CAGATGGCTG ATAAAATTCT GGAGCTGGAG 
GAGAAACATC GGGAGGCTCA GGTCTCAGCT CAACATCTAG AAGTACACTT GAAACAGAAA 
GAACAGCACT ACGAGGAAAA GATCAAGGTA TTGGACAATC AGATAAAGAA GGACCTGGCC 
GACAAGGAGA GCCTGGAGAA CATGATGCAG AGACACGAGG AGGAGGCCCA CGAGAAGGGC 
AAGATCCTCA GCGAGCAGAA GGCGATGATC AACGCGATGG ATTCCAAGAT CCGATCCCTG 
GAGCAGAGGA TCGTGGAGCT GTCGGAAGCC AACAAGCTTG CGGCAAACAG CAGTCTCTTC 
ACCCAGAGGA ACATGAAGGC CCAGGAAGAG ATGATCTCAG AACTCAGGCA GCAGAAATTT 
TACCTGGAGA CGCAGGCCGG AAAGCTGGAG GCCCAGAACC GAAAGCTGGA AGAGCAACTG 
GAGAAGATCA GCCACCAAGA TCACAGTGAC AAGAGTCGGC TGCTGGAGCT GGAAACAAGG 
CTGAGGGAGG TCAGCCTGGA GCACGAGGAG CAGAAGCTGG AGCTGAAGCG GCAGCTCACG 
GAGCTGCAGC TGTCCCTGCA GGAGCGCGAG TCCCAGCTGA CGGCCCTGCA GGCCGCCCGG 
GCAGCGCTGG AGAGCCAGCT CCGCCAGGCG AAGACAGAGC TGGAGGAGAC AACCGCGGAA 
GCGGAGGAGG AGATCCAGGC GCTCACGGCA CATCGAGATG AGATCCAGCG CAAATTCGAT 
GCCCTTCGCA ACAGCTGCAC GGTCATCACC GACCTGGAGG AGCAGCTGAA CCAGCTCACC 
GAGGACAACG CCGAGCTCAA CAACCAAAAC TTCTACCTGT CCAAACAACT CGATGAGGCT 
TCCGGGGCCA ATGACGAGAT TGTGCAGCTG CGGAGTGAGG TGGACCATCT CCGCCGTGAG 
ATCACGGAGC GGGAGATGCA GCTCACCAGC CAGAAGCAAA CGATGGAGGC TCTGAAGACG 
ACATGCACCA TGCTGGAGGA GCAGGTCCTG GACCTGGAGG CCCTGAACGA CGAGCTGCTG 
GAGAAGGAGC GCCAGTGGGA GGCCTGGCGG AGCGTCCTCG GCGACGAGAA GTCCCAGTTC 
GAGTGTCGAG TTCGAGAGCT ACAGAGGATG CTGGACACCG AGAAGCAGAG CAGGGCTAGG 
GCCGATCAGC GGATCACCGA GTCGCGCCAG GTGGTGGAGT TGGCGGTGAA GGAACACAAG 
GCCGAGATTC TTGCTCTGCA GCAGGCTCTC AAGGAGCAGA AGCTCAAAGC CGAGAGCCTG 
TCGGACAAGC TCAACGACCT GGAGAAGAAG CACGCCATGC TGGAGATGAA CGCCCGGAGC 
TTACAGCAGA AACTAGAGAC AGAGCGGGAG CTCAAACAGA GGCTTCTGGA GGAGCAAGCC 
AAATTGCAGC AGCAGATGGA CCTGCAGAAG AACCACATCT TCAGACTGAC GCAAGGGCTG 
CAGGAGGCGC TGGACCGGGC GGATCTGCTG AAGACAGAAA GGAGCGACCT GGAGTACCAG 
CTGGAAAACA TTCAGGTTCT CTACTCTCAC GAGAAAGTGA AAATGGAAGG CACAATCTCT 
CAGCAAACCA AACTCATTGA TTTCCTGCAA GCCAAAATGG ACCAGCCTGC TAAAAAGAAA 
AAGGTTCCTC TGCAGTACAA TGAGCTGAAG CTAGCCCTGG AAAAGGAGAA AGCCCGATGC 
GCGGAGCTGG AGGAGGCCCT TCAGAAGACC CGCATCGAAC TCCGCTCTGC GCGGGAGGAA 
GCTGCCCACC GCAAAGCCAC GGACCACCCG CACCCGTCTA CGCCAGCCAC TGCGAGGCAG 
CAGATCGCCA TGTCTGCCAT TGTGCGGTCG CCCGAGCACC AGCCCAGTGC CATGAGCCTG 
CTTGCCCCAC CATCCAGCCG CAGAAAGGAG TCGTCAACTC CAGAGGAATT CAGCCGGCGT 
CTGAAAGAGC GCATGCACCA CAACATCCCT CACCGGTTTA ATGTGGGCCT GAACATGAGA 
GCCACCAAGT GCGCCGTGTG TCTGGATACT GTGCACTTTG GACGCCAGGC ATCCAAATGC 
CTCGAATGTC AAGTGATGTG TCACCCCAAA TGCTCCACCT GCTTGCCCGC TACCTGTGGC 
CTGCCAGCTG AATATGCCAC ACACTTCACT GAGGCCTTCT GCCGGGACAA AATGAACTCC 
CCGGGGCTCC AGAGCAAGGA GCCTGGCAGC AGCTTGCACC TGGAAGGGTG GATGAAAGTG 
CCCAGAAATA ACAAACGGGG ACAGCAAGGC TGGGACAGGA AGTACATTGT TCTGGAGGGG 
TCAAAAGTCC TCATCTATGA CAATGAAGCC AGAGAAGCTG GACAGAGGCC GGTGGAAGAA 
TTTGAGCTGT GCCTTCCTGA CGGGGACGTA TCTATTCATG GTGCCGTTGG TGCTTCAGAA 
CTTGCAAATA CCGCCAAAGC AGATGTCCCA TACATCCTGA AGATGGAGTC TCACCCACAC 
ACCACCTGCT GGCCTGGGAG AACCCTCTAC TTGCTAGCAC CCAGCTTCCC CGACAAGCAG 
CGCTGGGTCA CCGCCTTAGA ATCTGTCGTC GCAGGTGGGA GAGTTTCTAG GGAAAAGGCC 
GAAGCCGATG CTAAATTACT TGGAAACTCT CTGCTGAAAC TGGAGGGCGA TGACCGGCTT 
GACATGAACT GCACCCTGCC CTTCAGTGAC CAGGTAGTGC TGGTGGGCAC GGAGGAAGGC 
CTCTACGCCC TGAATGTCTT GAAAAACTCC TTAACCCACA TCCCAGGGAT TGGCGCAGTC 
TTCCAAATTT ACATCATCAA GGACCTGGAG AAGCTGCTCA TGATAGCAGG GGAAGAGCGG 
GCTCTGTGCC TGGTGGACGT GAAGAAGGTG AAGCAGTCCC TGGCGCAGTC ACACCTGCCT 
GCCCAGCCCG ACGTCTCCCC CAACATATTC GAAGCCGTCA AAGGCTGCCA CTTGTTCGCT 
GCTGGCAAGA TCGAGAACAG CCTGTGCATC TGCGCCGCTA TGCCAAGCAA AGTCGTCATC 
CTCCGCTACA ATGACAACCT CAGCAAGTAC TGCATCCGCA AGGAGATCGA GACCTCAGAG 
CCCTGCAGCT GTATCCACTT CACCAACTAC AGCATCCTCA TTGGGACCAA CAAATTCTAT 
GAGATCGACA TGAAGCAGTA CACGCTTGAC GAGTTCCTGG ACAAGAACGA CCATTCCTTG 
GCACCTGCTG TGTTCGCCTC CTCGTCCAAC AGCTTCCCTG TCTCCATTGT GCAGGCGAAC 
AGCGCCGGGC AGCGAGAAGA ATACCTGCTG TGCTTCCACG AATTTGGGGT GTTCGTGGAT 
TCTTACGGAA GACGTAGCCG CACAGATGAT CTCAAGTGGA GTCGCTTACC TCTGGCCTTC 
GCCTACAGAG AACCTTATCT GTTTGTGACT CACTTCAACT CCCTGGAAGT CATTGAGATC 
CAGGCCAGAT CCTCACTGGG GAGCCCTGCC CGAGCATATC TGGAAATTCC AAACCCTCGC 
TACCTGGGCC CCGCGATTTC CTCCGGAGCG ATTTACCTGG CCTCCTCATA CCAGGACAAG 
TTAAGGGTCA TATGCTGCAA AGGAAACCTC GTGAAGGAGT CAGGCACTGA GCAGCACCGG 
GTGCCCTCCA CCTCCCGCAG CAGCCCCAAC AAGCGAGGCC CACCAACATA CAACGAGCAC 
ATCACCAAAC GCGTGGCCTC CAGCCCGGCG CCACCGGAAG GCCCCAGCCA CCCCCGAGAG 
CCAAGCACAC CGCACCGCTA CCGAGACAGA GAGGGTCGGA CAGAGCTGCG CAGGGACAAG 
TCTCCAGGCC GCCCTCTGGA GCGGGAGAAG TCCCCAGGCC GAATGCTCAG CACTAGGAGA 
GAGCGGTCCC CAGGGAGACT GTTTGAAGAC AGCAGCAGGG GCCGGCTGCC TGCAGGAGCA 
GTGAGGACCC CACTGTCCCA GGTTAACAAG GTCTGGGACC AGTCTTCAGT ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cit ( XM_006530141.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006530204.1 Mus musculus citron (Cit), transcript variant X15, mRNA.

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CloneID	OMu00646
Clone ID Related Accession (Same CDS sequence)	XM_006530141.2 , XM_006530141.4 , NM_007708.3 , XM_006530141.3
Accession Version	XM_006530141.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6168bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	citron Rho-interacting kinase isoform X11
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000071.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006530141.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTGAAGT TCAAGTATGG TGTGCGGAAC CCGCCGGAGG CCAGTGCCTC CGAGCCCATT 
GCCAGTCGGG CCTCCAGGCT AAATCTCTTC TTCCAGGGGA AACCGCCCCT CATGACTCAA 
CAGCAGATGT CTGCTCTTTC CCGGGAAGGG ATGCTAGACG CCCTCTTCGC TCTCTTTGAA 
GAGTGCAGCC AACCCGCCCT GATGAAGATG AAGCACGTGA GCAGCTTTGT CCAGAAGTAT 
TCCGACACCA TAGCCGAGTT GCGGGAGCTG CAGCCGTCGG CGAGAGACTT CGAAGTTCGA 
AGCCTTGTGG GCTGTGGTCA CTTCGCTGAA GTGCAGGTGG TTAGAGAGAA GGCGACCGGG 
GACGTCTATG CCATGAAAAT CATGAAGAAG AAGGCTTTGC TGGCCCAGGA ACAGGTTTCA 
TTTTTCGAGG AGGAGAGGAA CATATTATCT CGGAGCACGA GTCCTTGGAT CCCCCAGTTA 
CAGTACGCCT TTCAGGACAA AAATAACCTT TACCTGGTCA TGGAATATCA GCCTGGAGGG 
GATTTGCTGT CGCTTCTGAA CAGATACGAG GACCAATTAG ATGAGAGCAT GATCCAGTTT 
TACCTTGCTG AGCTGATTTT GGCTGTCCAC AGCGTGCACC AGATGGGATA TGTGCATCGA 
GACATCAAGC CCGAGAACAT CCTCATCGAC CGGACGGGAC ACATCAAGCT GGTGGATTTT 
GGATCAGCCG CTAAGATGAA TTCAAATAAG GTGGATGCCA AACTCCCCAT TGGGACCCCG 
GATTACATGG CTCCGGAAGT GTTGACCGTG ATGAACGAGG ACCGAAGGGG CACATACGGC 
TTGGACTGTG ACTGGTGGTC TGTCGGAGTT GTTGCTTATG AGATGGTTTA TGGGAAGACC 
CCATTCACAG AGGGAACCTC CGCCCGGACC TTCAACAACA TCATGAACTT CCAGCGGTTT 
TTGAAGTTCC CAGATGACCC CAAAGTTAGC AGTGAGCTCC TTGATCTGCT TCAGAGTCTG 
CTGTGTGTCC AGAAAGAGAG ACTGAAGTTC GAGGGTCTCT GCTGCCACCC TTTCTTTGCC 
AGAACGGACT GGAACAACAT CCGTAACTCT CCTCCCCCCT TCGTCCCCAC CCTCAAGTCT 
GACGATGACA CCTCCAATTT TGATGAACCA GAGAAGAATT CGTGGGTTTC ATCCTCTGTG 
TGCCAGCTGA GCCCCTCGGG CTTCTCAGGC GAAGAGCTGC CGTTTGTGGG ATTTTCGTAC 
AGCAAGGCAC TGGGGTATCT TGGTAGATCT GAGTCTGTCG TGTCGAGTCT GGACTCCCCT 
GCCAAGGTTA GCTCCATGGA AAAGAAACTT CTCATCAAAA GCAAAGAGCT CCAAGACTCC 
CAGGACAAGT GTCACAAGAT GGAGCAGGAA ATGACCCGGT TACATCGCAG AGTGTCAGAG 
GTGGAGGCTG TGCTTAGTCA GAAGGAGGTG GAGCTGAAGG CCTCTGAGAC TCAGAGATCC 
CTCCTGGAGC AGGACCTTGC TACCTACATC ACAGAATGCA GTAGCTTAAA GCGAAGTTTG 
GAGCAAGCGC GGATGGAGGT GTCCCAGGAG GATGACAAAG CTCTGCAGCT TCTCCACGAC 
ATCCGAGAGC AGAGCCGGAA GCTCCAGGAG ATCAAGGAGC AGGAGTACCA GGCTCAGGTG 
GAGGAGATGA GGCTGATGAT GAATCAGCTG GAAGAAGACC TGGTGTCAGC CCGCAGACGC 
AGCGATCTCT ACGAGTCTGA GCTGAGGGAG TCTCGGCTTG CCGCCGAGGA ATTCAAGCGG 
AAGGCAAACG AATGTCAGCA CAAACTGATG AAGGCTAAGG ACCAAGGGAA GCCTGAAGTG 
GGAGAATATT CCAAACTGGA GAAGATCAAT GCTGAGCAGC AGCTGAAGAT CCAGGAGCTC 
CAGGAGAAGC TGGAGAAGGC TGTAAAAGCC AGCACAGAGG CCACCGAGCT CCTGCAGAAT 
ATCCGCCAGG CAAAGGAGCG AGCAGAGCGG GAGCTAGAGA AGCTACACAA CCGGGAAGAC 
TCCTCCGAGG GCATCAAAAA GAAGCTGGTG GAAGCCGAGG AACGCCGCCA CTCCCTGGAG 
AACAAGGTAA AGAGACTAGA GACCATGGAG CGTAGAGAGA ACAGACTGAA GGATGACATC 
CAGACAAAGT CCGAACAGAT CCAGCAGATG GCTGATAAAA TTCTGGAGCT GGAGGAGAAA 
CATCGGGAGG CTCAGGTCTC AGCTCAACAT CTAGAAGTAC ACTTGAAACA GAAAGAACAG 
CACTACGAGG AAAAGATCAA GGTATTGGAC AATCAGATAA AGAAGGACCT GGCCGACAAG 
GAGAGCCTGG AGAACATGAT GCAGAGACAC GAGGAGGAGG CCCACGAGAA GGGCAAGATC 
CTCAGCGAGC AGAAGGCGAT GATCAACGCG ATGGATTCCA AGATCCGATC CCTGGAGCAG 
AGGATCGTGG AGCTGTCGGA AGCCAACAAG CTTGCGGCAA ACAGCAGTCT CTTCACCCAG 
AGGAACATGA AGGCCCAGGA AGAGATGATC TCAGAACTCA GGCAGCAGAA ATTTTACCTG 
GAGACGCAGG CCGGAAAGCT GGAGGCCCAG AACCGAAAGC TGGAAGAGCA ACTGGAGAAG 
ATCAGCCACC AAGATCACAG TGACAAGAGT CGGCTGCTGG AGCTGGAAAC AAGGCTGAGG 
GAGGTCAGCC TGGAGCACGA GGAGCAGAAG CTGGAGCTGA AGCGGCAGCT CACGGAGCTG 
CAGCTGTCCC TGCAGGAGCG CGAGTCCCAG CTGACGGCCC TGCAGGCCGC CCGGGCAGCG 
CTGGAGAGCC AGCTCCGCCA GGCGAAGACA GAGCTGGAGG AGACAACCGC GGAAGCGGAG 
GAGGAGATCC AGGCGCTCAC GGCACATCGA GATGAGATCC AGCGCAAATT CGATGCCCTT 
CGCAACAGCT GCACGGTCAT CACCGACCTG GAGGAGCAGC TGAACCAGCT CACCGAGGAC 
AACGCCGAGC TCAACAACCA AAACTTCTAC CTGTCCAAAC AACTCGATGA GGCTTCCGGG 
GCCAATGACG AGATTGTGCA GCTGCGGAGT GAGGTGGACC ATCTCCGCCG TGAGATCACG 
GAGCGGGAGA TGCAGCTCAC CAGCCAGAAG CAAACGATGG AGGCTCTGAA GACGACATGC 
ACCATGCTGG AGGAGCAGGT CCTGGACCTG GAGGCCCTGA ACGACGAGCT GCTGGAGAAG 
GAGCGCCAGT GGGAGGCCTG GCGGAGCGTC CTCGGCGACG AGAAGTCCCA GTTCGAGTGT 
CGAGTTCGAG AGCTACAGAG GATGCTGGAC ACCGAGAAGC AGAGCAGGGC TAGGGCCGAT 
CAGCGGATCA CCGAGTCGCG CCAGGTGGTG GAGTTGGCGG TGAAGGAACA CAAGGCCGAG 
ATTCTTGCTC TGCAGCAGGC TCTCAAGGAG CAGAAGCTCA AAGCCGAGAG CCTGTCGGAC 
AAGCTCAACG ACCTGGAGAA GAAGCACGCC ATGCTGGAGA TGAACGCCCG GAGCTTACAG 
CAGAAACTAG AGACAGAGCG GGAGCTCAAA CAGAGGCTTC TGGAGGAGCA AGCCAAATTG 
CAGCAGCAGA TGGACCTGCA GAAGAACCAC ATCTTCAGAC TGACGCAAGG GCTGCAGGAG 
GCGCTGGACC GGGCGGATCT GCTGAAGACA GAAAGGAGCG ACCTGGAGTA CCAGCTGGAA 
AACATTCAGG TTCTCTACTC TCACGAGAAA GTGAAAATGG AAGGCACAAT CTCTCAGCAA 
ACCAAACTCA TTGATTTCCT GCAAGCCAAA ATGGACCAGC CTGCTAAAAA GAAAAAGGTT 
CCTCTGCAGT ACAATGAGCT GAAGCTAGCC CTGGAAAAGG AGAAAGCCCG ATGCGCGGAG 
CTGGAGGAGG CCCTTCAGAA GACCCGCATC GAACTCCGCT CTGCGCGGGA GGAAGCTGCC 
CACCGCAAAG CCACGGACCA CCCGCACCCG TCTACGCCAG CCACTGCGAG GCAGCAGATC 
GCCATGTCTG CCATTGTGCG GTCGCCCGAG CACCAGCCCA GTGCCATGAG CCTGCTTGCC 
CCACCATCCA GCCGCAGAAA GGAGTCGTCA ACTCCAGAGG AATTCAGCCG GCGTCTGAAA 
GAGCGCATGC ACCACAACAT CCCTCACCGG TTTAATGTGG GCCTGAACAT GAGAGCCACC 
AAGTGCGCCG TGTGTCTGGA TACTGTGCAC TTTGGACGCC AGGCATCCAA ATGCCTCGAA 
TGTCAAGTGA TGTGTCACCC CAAATGCTCC ACCTGCTTGC CCGCTACCTG TGGCCTGCCA 
GCTGAATATG CCACACACTT CACTGAGGCC TTCTGCCGGG ACAAAATGAA CTCCCCGGGG 
CTCCAGAGCA AGGAGCCTGG CAGCAGCTTG CACCTGGAAG GGTGGATGAA AGTGCCCAGA 
AATAACAAAC GGGGACAGCA AGGCTGGGAC AGGAAGTACA TTGTTCTGGA GGGGTCAAAA 
GTCCTCATCT ATGACAATGA AGCCAGAGAA GCTGGACAGA GGCCGGTGGA AGAATTTGAG 
CTGTGCCTTC CTGACGGGGA CGTATCTATT CATGGTGCCG TTGGTGCTTC AGAACTTGCA 
AATACCGCCA AAGCAGATGT CCCATACATC CTGAAGATGG AGTCTCACCC ACACACCACC 
TGCTGGCCTG GGAGAACCCT CTACTTGCTA GCACCCAGCT TCCCCGACAA GCAGCGCTGG 
GTCACCGCCT TAGAATCTGT CGTCGCAGGT GGGAGAGTTT CTAGGGAAAA GGCCGAAGCC 
GATGCTAAAT TACTTGGAAA CTCTCTGCTG AAACTGGAGG GCGATGACCG GCTTGACATG 
AACTGCACCC TGCCCTTCAG TGACCAGGTA GTGCTGGTGG GCACGGAGGA AGGCCTCTAC 
GCCCTGAATG TCTTGAAAAA CTCCTTAACC CACATCCCAG GGATTGGCGC AGTCTTCCAA 
ATTTACATCA TCAAGGACCT GGAGAAGCTG CTCATGATAG CAGGGGAAGA GCGGGCTCTG 
TGCCTGGTGG ACGTGAAGAA GGTGAAGCAG TCCCTGGCGC AGTCACACCT GCCTGCCCAG 
CCCGACGTCT CCCCCAACAT ATTCGAAGCC GTCAAAGGCT GCCACTTGTT CGCTGCTGGC 
AAGATCGAGA ACAGCCTGTG CATCTGCGCC GCTATGCCAA GCAAAGTCGT CATCCTCCGC 
TACAATGACA ACCTCAGCAA GTACTGCATC CGCAAGGAGA TCGAGACCTC AGAGCCCTGC 
AGCTGTATCC ACTTCACCAA CTACAGCATC CTCATTGGGA CCAACAAATT CTATGAGATC 
GACATGAAGC AGTACACGCT TGACGAGTTC CTGGACAAGA ACGACCATTC CTTGGCACCT 
GCTGTGTTCG CCTCCTCGTC CAACAGCTTC CCTGTCTCCA TTGTGCAGGC GAACAGCGCC 
GGGCAGCGAG AAGAATACCT GCTGTGCTTC CACGAATTTG GGGTGTTCGT GGATTCTTAC 
GGAAGACGTA GCCGCACAGA TGATCTCAAG TGGAGTCGCT TACCTCTGGC CTTCGCCTAC 
AGAGAACCTT ATCTGTTTGT GACTCACTTC AACTCCCTGG AAGTCATTGA GATCCAGGCC 
AGATCCTCAC TGGGGAGCCC TGCCCGAGCA TATCTGGAAA TTCCAAACCC TCGCTACCTG 
GGCCCCGCGA TTTCCTCCGG AGCGATTTAC CTGGCCTCCT CATACCAGGA CAAGTTAAGG 
GTCATATGCT GCAAAGGAAA CCTCGTGAAG GAGTCAGGCA CTGAGCAGCA CCGGGTGCCC 
TCCACCTCCC GCAGCAGCCC CAACAAGCGA GGCCCACCAA CATACAACGA GCACATCACC 
AAACGCGTGG CCTCCAGCCC GGCGCCACCG GAAGGCCCCA GCCACCCCCG AGAGCCAAGC 
ACACCGCACC GCTACCGAGA CAGAGAGGGT CGGACAGAGC TGCGCAGGGA CAAGTCTCCA 
GGCCGCCCTC TGGAGCGGGA GAAGTCCCCA GGCCGAATGC TCAGCACTAG GAGAGAGCGG 
TCCCCAGGGA GACTGTTTGA AGACAGCAGC AGGGGCCGGC TGCCTGCAGG AGCAGTGAGG 
ACCCCACTGT CCCAGGTTAA CAAGGTCTGG GACCAGTCTT CAGTATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006530204.1
CDS	157..6324
Translation	MLKFKYGVRNPPEASASEPIASRASRLNLFFQGKPPLMTQQQMSALSREGMLDALFALFEECSQPALMKMKHVSSFVQKYSDTIAELRELQPSARDFEVRSLVGCGHFAEVQVVREKATGDVYAMKIMKKKALLAQEQVSFFEEERNILSRSTSPWIPQLQYAFQDKNNLYLVMEYQPGGDLLSLLNRYEDQLDESMIQFYLAELILAVHSVHQMGYVHRDIKPENILIDRTGHIKLVDFGSAAKMNSNKVDAKLPIGTPDYMAPEVLTVMNEDRRGTYGLDCDWWSVGVVAYEMVYGKTPFTEGTSARTFNNIMNFQRFLKFPDDPKVSSELLDLLQSLLCVQKERLKFEGLCCHPFFARTDWNNIRNSPPPFVPTLKSDDDTSNFDEPEKNSWVSSSVCQLSPSGFSGEELPFVGFSYSKALGYLGRSESVVSSLDSPAKVSSMEKKLLIKSKELQDSQDKCHKMEQEMTRLHRRVSEVEAVLSQKEVELKASETQRSLLEQDLATYITECSSLKRSLEQARMEVSQEDDKALQLLHDIREQSRKLQEIKEQEYQAQVEEMRLMMNQLEEDLVSARRRSDLYESELRESRLAAEEFKRKANECQHKLMKAKDQGKPEVGEYSKLEKINAEQQLKIQELQEKLEKAVKASTEATELLQNIRQAKERAERELEKLHNREDSSEGIKKKLVEAEERRHSLENKVKRLETMERRENRLKDDIQTKSEQIQQMADKILELEEKHREAQVSAQHLEVHLKQKEQHYEEKIKVLDNQIKKDLADKESLENMMQRHEEEAHEKGKILSEQKAMINAMDSKIRSLEQRIVELSEANKLAANSSLFTQRNMKAQEEMISELRQQKFYLETQAGKLEAQNRKLEEQLEKISHQDHSDKSRLLELETRLREVSLEHEEQKLELKRQLTELQLSLQERESQLTALQAARAALESQLRQAKTELEETTAEAEEEIQALTAHRDEIQRKFDALRNSCTVITDLEEQLNQLTEDNAELNNQNFYLSKQLDEASGANDEIVQLRSEVDHLRREITEREMQLTSQKQTMEALKTTCTMLEEQVLDLEALNDELLEKERQWEAWRSVLGDEKSQFECRVRELQRMLDTEKQSRARADQRITESRQVVELAVKEHKAEILALQQALKEQKLKAESLSDKLNDLEKKHAMLEMNARSLQQKLETERELKQRLLEEQAKLQQQMDLQKNHIFRLTQGLQEALDRADLLKTERSDLEYQLENIQVLYSHEKVKMEGTISQQTKLIDFLQAKMDQPAKKKKVPLQYNELKLALEKEKARCAELEEALQKTRIELRSAREEAAHRKATDHPHPSTPATARQQIAMSAIVRSPEHQPSAMSLLAPPSSRRKESSTPEEFSRRLKERMHHNIPHRFNVGLNMRATKCAVCLDTVHFGRQASKCLECQVMCHPKCSTCLPATCGLPAEYATHFTEAFCRDKMNSPGLQSKEPGSSLHLEGWMKVPRNNKRGQQGWDRKYIVLEGSKVLIYDNEAREAGQRPVEEFELCLPDGDVSIHGAVGASELANTAKADVPYILKMESHPHTTCWPGRTLYLLAPSFPDKQRWVTALESVVAGGRVSREKAEADAKLLGNSLLKLEGDDRLDMNCTLPFSDQVVLVGTEEGLYALNVLKNSLTHIPGIGAVFQIYIIKDLEKLLMIAGEERALCLVDVKKVKQSLAQSHLPAQPDVSPNIFEAVKGCHLFAAGKIENSLCICAAMPSKVVILRYNDNLSKYCIRKEIETSEPCSCIHFTNYSILIGTNKFYEIDMKQYTLDEFLDKNDHSLAPAVFASSSNSFPVSIVQANSAGQREEYLLCFHEFGVFVDSYGRRSRTDDLKWSRLPLAFAYREPYLFVTHFNSLEVIEIQARSSLGSPARAYLEIPNPRYLGPAISSGAIYLASSYQDKLRVICCKGNLVKESGTEQHRVPSTSRSSPNKRGPPTYNEHITKRVASSPAPPEGPSHPREPSTPHRYRDREGRTELRRDKSPGRPLEREKSPGRMLSTRRERSPGRLFEDSSRGRLPAGAVRTPLSQVNKVWDQSSV

Target ORF information:

RefSeq Version	XM_006530141.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus citron (Cit), transcript variant X15, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006530141.4

ORF Insert Sequence:

ATGTTGAAGT TCAAGTATGG TGTGCGGAAC CCGCCGGAGG CCAGTGCCTC CGAGCCCATT 
GCCAGTCGGG CCTCCAGGCT AAATCTCTTC TTCCAGGGGA AACCGCCCCT CATGACTCAA 
CAGCAGATGT CTGCTCTTTC CCGGGAAGGG ATGCTAGACG CCCTCTTCGC TCTCTTTGAA 
GAGTGCAGCC AACCCGCCCT GATGAAGATG AAGCACGTGA GCAGCTTTGT CCAGAAGTAT 
TCCGACACCA TAGCCGAGTT GCGGGAGCTG CAGCCGTCGG CGAGAGACTT CGAAGTTCGA 
AGCCTTGTGG GCTGTGGTCA CTTCGCTGAA GTGCAGGTGG TTAGAGAGAA GGCGACCGGG 
GACGTCTATG CCATGAAAAT CATGAAGAAG AAGGCTTTGC TGGCCCAGGA ACAGGTTTCA 
TTTTTCGAGG AGGAGAGGAA CATATTATCT CGGAGCACGA GTCCTTGGAT CCCCCAGTTA 
CAGTACGCCT TTCAGGACAA AAATAACCTT TACCTGGTCA TGGAATATCA GCCTGGAGGG 
GATTTGCTGT CGCTTCTGAA CAGATACGAG GACCAATTAG ATGAGAGCAT GATCCAGTTT 
TACCTTGCTG AGCTGATTTT GGCTGTCCAC AGCGTGCACC AGATGGGATA TGTGCATCGA 
GACATCAAGC CCGAGAACAT CCTCATCGAC CGGACGGGAC ACATCAAGCT GGTGGATTTT 
GGATCAGCCG CTAAGATGAA TTCAAATAAG GTGGATGCCA AACTCCCCAT TGGGACCCCG 
GATTACATGG CTCCGGAAGT GTTGACCGTG ATGAACGAGG ACCGAAGGGG CACATACGGC 
TTGGACTGTG ACTGGTGGTC TGTCGGAGTT GTTGCTTATG AGATGGTTTA TGGGAAGACC 
CCATTCACAG AGGGAACCTC CGCCCGGACC TTCAACAACA TCATGAACTT CCAGCGGTTT 
TTGAAGTTCC CAGATGACCC CAAAGTTAGC AGTGAGCTCC TTGATCTGCT TCAGAGTCTG 
CTGTGTGTCC AGAAAGAGAG ACTGAAGTTC GAGGGTCTCT GCTGCCACCC TTTCTTTGCC 
AGAACGGACT GGAACAACAT CCGTAACTCT CCTCCCCCCT TCGTCCCCAC CCTCAAGTCT 
GACGATGACA CCTCCAATTT TGATGAACCA GAGAAGAATT CGTGGGTTTC ATCCTCTGTG 
TGCCAGCTGA GCCCCTCGGG CTTCTCAGGC GAAGAGCTGC CGTTTGTGGG ATTTTCGTAC 
AGCAAGGCAC TGGGGTATCT TGGTAGATCT GAGTCTGTCG TGTCGAGTCT GGACTCCCCT 
GCCAAGGTTA GCTCCATGGA AAAGAAACTT CTCATCAAAA GCAAAGAGCT CCAAGACTCC 
CAGGACAAGT GTCACAAGAT GGAGCAGGAA ATGACCCGGT TACATCGCAG AGTGTCAGAG 
GTGGAGGCTG TGCTTAGTCA GAAGGAGGTG GAGCTGAAGG CCTCTGAGAC TCAGAGATCC 
CTCCTGGAGC AGGACCTTGC TACCTACATC ACAGAATGCA GTAGCTTAAA GCGAAGTTTG 
GAGCAAGCGC GGATGGAGGT GTCCCAGGAG GATGACAAAG CTCTGCAGCT TCTCCACGAC 
ATCCGAGAGC AGAGCCGGAA GCTCCAGGAG ATCAAGGAGC AGGAGTACCA GGCTCAGGTG 
GAGGAGATGA GGCTGATGAT GAATCAGCTG GAAGAAGACC TGGTGTCAGC CCGCAGACGC 
AGCGATCTCT ACGAGTCTGA GCTGAGGGAG TCTCGGCTTG CCGCCGAGGA ATTCAAGCGG 
AAGGCAAACG AATGTCAGCA CAAACTGATG AAGGCTAAGG ACCAAGGGAA GCCTGAAGTG 
GGAGAATATT CCAAACTGGA GAAGATCAAT GCTGAGCAGC AGCTGAAGAT CCAGGAGCTC 
CAGGAGAAGC TGGAGAAGGC TGTAAAAGCC AGCACAGAGG CCACCGAGCT CCTGCAGAAT 
ATCCGCCAGG CAAAGGAGCG AGCAGAGCGG GAGCTAGAGA AGCTACACAA CCGGGAAGAC 
TCCTCCGAGG GCATCAAAAA GAAGCTGGTG GAAGCCGAGG AACGCCGCCA CTCCCTGGAG 
AACAAGGTAA AGAGACTAGA GACCATGGAG CGTAGAGAGA ACAGACTGAA GGATGACATC 
CAGACAAAGT CCGAACAGAT CCAGCAGATG GCTGATAAAA TTCTGGAGCT GGAGGAGAAA 
CATCGGGAGG CTCAGGTCTC AGCTCAACAT CTAGAAGTAC ACTTGAAACA GAAAGAACAG 
CACTACGAGG AAAAGATCAA GGTATTGGAC AATCAGATAA AGAAGGACCT GGCCGACAAG 
GAGAGCCTGG AGAACATGAT GCAGAGACAC GAGGAGGAGG CCCACGAGAA GGGCAAGATC 
CTCAGCGAGC AGAAGGCGAT GATCAACGCG ATGGATTCCA AGATCCGATC CCTGGAGCAG 
AGGATCGTGG AGCTGTCGGA AGCCAACAAG CTTGCGGCAA ACAGCAGTCT CTTCACCCAG 
AGGAACATGA AGGCCCAGGA AGAGATGATC TCAGAACTCA GGCAGCAGAA ATTTTACCTG 
GAGACGCAGG CCGGAAAGCT GGAGGCCCAG AACCGAAAGC TGGAAGAGCA ACTGGAGAAG 
ATCAGCCACC AAGATCACAG TGACAAGAGT CGGCTGCTGG AGCTGGAAAC AAGGCTGAGG 
GAGGTCAGCC TGGAGCACGA GGAGCAGAAG CTGGAGCTGA AGCGGCAGCT CACGGAGCTG 
CAGCTGTCCC TGCAGGAGCG CGAGTCCCAG CTGACGGCCC TGCAGGCCGC CCGGGCAGCG 
CTGGAGAGCC AGCTCCGCCA GGCGAAGACA GAGCTGGAGG AGACAACCGC GGAAGCGGAG 
GAGGAGATCC AGGCGCTCAC GGCACATCGA GATGAGATCC AGCGCAAATT CGATGCCCTT 
CGCAACAGCT GCACGGTCAT CACCGACCTG GAGGAGCAGC TGAACCAGCT CACCGAGGAC 
AACGCCGAGC TCAACAACCA AAACTTCTAC CTGTCCAAAC AACTCGATGA GGCTTCCGGG 
GCCAATGACG AGATTGTGCA GCTGCGGAGT GAGGTGGACC ATCTCCGCCG TGAGATCACG 
GAGCGGGAGA TGCAGCTCAC CAGCCAGAAG CAAACGATGG AGGCTCTGAA GACGACATGC 
ACCATGCTGG AGGAGCAGGT CCTGGACCTG GAGGCCCTGA ACGACGAGCT GCTGGAGAAG 
GAGCGCCAGT GGGAGGCCTG GCGGAGCGTC CTCGGCGACG AGAAGTCCCA GTTCGAGTGT 
CGAGTTCGAG AGCTACAGAG GATGCTGGAC ACCGAGAAGC AGAGCAGGGC TAGGGCCGAT 
CAGCGGATCA CCGAGTCGCG CCAGGTGGTG GAGTTGGCGG TGAAGGAACA CAAGGCCGAG 
ATTCTTGCTC TGCAGCAGGC TCTCAAGGAG CAGAAGCTCA AAGCCGAGAG CCTGTCGGAC 
AAGCTCAACG ACCTGGAGAA GAAGCACGCC ATGCTGGAGA TGAACGCCCG GAGCTTACAG 
CAGAAACTAG AGACAGAGCG GGAGCTCAAA CAGAGGCTTC TGGAGGAGCA AGCCAAATTG 
CAGCAGCAGA TGGACCTGCA GAAGAACCAC ATCTTCAGAC TGACGCAAGG GCTGCAGGAG 
GCGCTGGACC GGGCGGATCT GCTGAAGACA GAAAGGAGCG ACCTGGAGTA CCAGCTGGAA 
AACATTCAGG TTCTCTACTC TCACGAGAAA GTGAAAATGG AAGGCACAAT CTCTCAGCAA 
ACCAAACTCA TTGATTTCCT GCAAGCCAAA ATGGACCAGC CTGCTAAAAA GAAAAAGGTT 
CCTCTGCAGT ACAATGAGCT GAAGCTAGCC CTGGAAAAGG AGAAAGCCCG ATGCGCGGAG 
CTGGAGGAGG CCCTTCAGAA GACCCGCATC GAACTCCGCT CTGCGCGGGA GGAAGCTGCC 
CACCGCAAAG CCACGGACCA CCCGCACCCG TCTACGCCAG CCACTGCGAG GCAGCAGATC 
GCCATGTCTG CCATTGTGCG GTCGCCCGAG CACCAGCCCA GTGCCATGAG CCTGCTTGCC 
CCACCATCCA GCCGCAGAAA GGAGTCGTCA ACTCCAGAGG AATTCAGCCG GCGTCTGAAA 
GAGCGCATGC ACCACAACAT CCCTCACCGG TTTAATGTGG GCCTGAACAT GAGAGCCACC 
AAGTGCGCCG TGTGTCTGGA TACTGTGCAC TTTGGACGCC AGGCATCCAA ATGCCTCGAA 
TGTCAAGTGA TGTGTCACCC CAAATGCTCC ACCTGCTTGC CCGCTACCTG TGGCCTGCCA 
GCTGAATATG CCACACACTT CACTGAGGCC TTCTGCCGGG ACAAAATGAA CTCCCCGGGG 
CTCCAGAGCA AGGAGCCTGG CAGCAGCTTG CACCTGGAAG GGTGGATGAA AGTGCCCAGA 
AATAACAAAC GGGGACAGCA AGGCTGGGAC AGGAAGTACA TTGTTCTGGA GGGGTCAAAA 
GTCCTCATCT ATGACAATGA AGCCAGAGAA GCTGGACAGA GGCCGGTGGA AGAATTTGAG 
CTGTGCCTTC CTGACGGGGA CGTATCTATT CATGGTGCCG TTGGTGCTTC AGAACTTGCA 
AATACCGCCA AAGCAGATGT CCCATACATC CTGAAGATGG AGTCTCACCC ACACACCACC 
TGCTGGCCTG GGAGAACCCT CTACTTGCTA GCACCCAGCT TCCCCGACAA GCAGCGCTGG 
GTCACCGCCT TAGAATCTGT CGTCGCAGGT GGGAGAGTTT CTAGGGAAAA GGCCGAAGCC 
GATGCTAAAT TACTTGGAAA CTCTCTGCTG AAACTGGAGG GCGATGACCG GCTTGACATG 
AACTGCACCC TGCCCTTCAG TGACCAGGTA GTGCTGGTGG GCACGGAGGA AGGCCTCTAC 
GCCCTGAATG TCTTGAAAAA CTCCTTAACC CACATCCCAG GGATTGGCGC AGTCTTCCAA 
ATTTACATCA TCAAGGACCT GGAGAAGCTG CTCATGATAG CAGGGGAAGA GCGGGCTCTG 
TGCCTGGTGG ACGTGAAGAA GGTGAAGCAG TCCCTGGCGC AGTCACACCT GCCTGCCCAG 
CCCGACGTCT CCCCCAACAT ATTCGAAGCC GTCAAAGGCT GCCACTTGTT CGCTGCTGGC 
AAGATCGAGA ACAGCCTGTG CATCTGCGCC GCTATGCCAA GCAAAGTCGT CATCCTCCGC 
TACAATGACA ACCTCAGCAA GTACTGCATC CGCAAGGAGA TCGAGACCTC AGAGCCCTGC 
AGCTGTATCC ACTTCACCAA CTACAGCATC CTCATTGGGA CCAACAAATT CTATGAGATC 
GACATGAAGC AGTACACGCT TGACGAGTTC CTGGACAAGA ACGACCATTC CTTGGCACCT 
GCTGTGTTCG CCTCCTCGTC CAACAGCTTC CCTGTCTCCA TTGTGCAGGC GAACAGCGCC 
GGGCAGCGAG AAGAATACCT GCTGTGCTTC CACGAATTTG GGGTGTTCGT GGATTCTTAC 
GGAAGACGTA GCCGCACAGA TGATCTCAAG TGGAGTCGCT TACCTCTGGC CTTCGCCTAC 
AGAGAACCTT ATCTGTTTGT GACTCACTTC AACTCCCTGG AAGTCATTGA GATCCAGGCC 
AGATCCTCAC TGGGGAGCCC TGCCCGAGCA TATCTGGAAA TTCCAAACCC TCGCTACCTG 
GGCCCCGCGA TTTCCTCCGG AGCGATTTAC CTGGCCTCCT CATACCAGGA CAAGTTAAGG 
GTCATATGCT GCAAAGGAAA CCTCGTGAAG GAGTCAGGCA CTGAGCAGCA CCGGGTGCCC 
TCCACCTCCC GCAGCAGCCC CAACAAGCGA GGCCCACCAA CATACAACGA GCACATCACC 
AAACGCGTGG CCTCCAGCCC GGCGCCACCG GAAGGCCCCA GCCACCCCCG AGAGCCAAGC 
ACACCGCACC GCTACCGAGA CAGAGAGGGT CGGACAGAGC TGCGCAGGGA CAAGTCTCCA 
GGCCGCCCTC TGGAGCGGGA GAAGTCCCCA GGCCGAATGC TCAGCACTAG GAGAGAGCGG 
TCCCCAGGGA GACTGTTTGA AGACAGCAGC AGGGGCCGGC TGCCTGCAGG AGCAGTGAGG 
ACCCCACTGT CCCAGGTTAA CAAGGTCTGG GACCAGTCTT CAGTATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cit ( XM_006530130.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006530193.1 Mus musculus citron (Cit), transcript variant X1, mRNA.

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CloneID	OMu35774
Clone ID Related Accession (Same CDS sequence)	XM_006530130.3 , XM_006530132.3 , XM_006530133.3 , XM_006530130.4 , XM_006530131.2 , XM_006530131.1 , XM_006530133.4 , XM_006530132.4
Accession Version	XM_006530130.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6372bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	citron Rho-interacting kinase isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000071.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006530130.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTGAAGT TCAAGTATGG TGTGCGGAAC CCGCCGGAGG CCAGTGCCTC CGAGCCCATT 
GCCAGTCGGG CCTCCAGGCT AAATCTCTTC TTCCAGGGGA AACCGCCCCT CATGACTCAA 
CAGCAGATGT CTGCTCTTTC CCGGGAAGGG ATGCTAGACG CCCTCTTCGC TCTCTTTGAA 
GAGTGCAGCC AACCCGCCCT GATGAAGATG AAGCACGTGA GCAGCTTTGT CCAGAAGTAT 
TCCGACACCA TAGCCGAGTT GCGGGAGCTG CAGCCGTCGG CGAGAGACTT CGAAGTTCGA 
AGCCTTGTGG GCTGTGGTCA CTTCGCTGAA GTGCAGGTGG TTAGAGAGAA GGCGACCGGG 
GACGTCTATG CCATGAAAAT CATGAAGAAG AAGGCTTTGC TGGCCCAGGA ACAGGTTTCA 
TTTTTCGAGG AGGAGAGGAA CATATTATCT CGGAGCACGA GTCCTTGGAT CCCCCAGTTA 
CAGTACGCCT TTCAGGACAA AAATAACCTT TACCTGGTCA TGGAATATCA GCCTGGAGGG 
GATTTGCTGT CGCTTCTGAA CAGATACGAG GACCAATTAG ATGAGAGCAT GATCCAGTTT 
TACCTTGCTG AGCTGATTTT GGCTGTCCAC AGCGTGCACC AGATGGGATA TGTGCATCGA 
GACATCAAGC CCGAGAACAT CCTCATCGAC CGGACGGGAC ACATCAAGCT GGTGGATTTT 
GGATCAGCCG CTAAGATGAA TTCAAATAAG GTGGATGCCA AACTCCCCAT TGGGACCCCG 
GATTACATGG CTCCGGAAGT GTTGACCGTG ATGAACGAGG ACCGAAGGGG CACATACGGC 
TTGGACTGTG ACTGGTGGTC TGTCGGAGTT GTTGCTTATG AGATGGTTTA TGGGAAGACC 
CCATTCACAG AGGGAACCTC CGCCCGGACC TTCAACAACA TCATGAACTT CCAGCGGTTT 
TTGAAGTTCC CAGATGACCC CAAAGTTAGC AGTGAGCTCC TTGATCTGCT TCAGAGTCTG 
CTGTGTGTCC AGAAAGAGAG ACTGAAGTTC GAGGGTCTCT GCTGCCACCC TTTCTTTGCC 
AGAACGGACT GGAACAACAT CCGTAACTCT CCTCCCCCCT TCGTCCCCAC CCTCAAGTCT 
GACGATGACA CCTCCAATTT TGATGAACCA GAGAAGAATT CGTGGGTTTC ATCCTCTGTG 
TGCCAGCTGA GCCCCTCGGG CTTCTCAGGC GAAGAGCTGC CGTTTGTGGG ATTTTCGTAC 
AGCAAGGCAC TGGGGTATCT TGGTAGATCT GAGTCTGTCG TGTCGAGTCT GGACTCCCCT 
GCCAAGGTTA GCTCCATGGA AAAGAAACTT CTCATCAAAA GCAAAGAGCT CCAAGACTCC 
CAGGACAAGT GTCACAAGAT GGAGCAGGAA ATGACCCGGT TACATCGCAG AGTGTCAGAG 
GTGGAGGCTG TGCTTAGTCA GAAGGAGGTG GAGCTGAAGG CCTCTGAGAC TCAGAGATCC 
CTCCTGGAGC AGGACCTTGC TACCTACATC ACAGAATGCA GTAGCTTAAA GCGAAGTTTG 
GAGCAAGCGC GGATGGAGGT GTCCCAGGAG GATGACAAAG CTCTGCAGCT TCTCCACGAC 
ATCCGAGAGC AGAGCCGGAA GCTCCAGGAG ATCAAGGAGC AGGAGTACCA GGCTCAGGTG 
GAGGAGATGA GGCTGATGAT GAATCAGCTG GAAGAAGACC TGGTGTCAGC CCGCAGACGC 
AGCGATCTCT ACGAGTCTGA GCTGAGGGAG TCTCGGCTTG CCGCCGAGGA ATTCAAGCGG 
AAGGCAAACG AATGTCAGCA CAAACTGATG AAGGTAGTCA GCCACCCTCC AAGAGGGGAC 
TCCGGGGGCA CGGCCCTGGA TGATCTTCAC AAGATGCAAG GCCACGCTGG CTTAACGAGT 
GCTAAGGACC AAGGGAAGCC TGAAGTGGGA GAATATTCCA AACTGGAGAA GATCAATGCT 
GAGCAGCAGC TGAAGATCCA GGAGCTCCAG GAGAAGCTGG AGAAGGCTGT AAAAGCCAGC 
ACAGAGGCCA CCGAGCTCCT GCAGAATATC CGCCAGGCAA AGGAGCGAGC AGAGCGGGAG 
CTAGAGAAGC TACACAACCG GGAAGACTCC TCCGAGGGCA TCAAAAAGAA GCTGGTGGAA 
GCCGAGGAAC GCCGCCACTC CCTGGAGAAC AAGGTAAAGA GACTAGAGAC CATGGAGCGT 
AGAGAGAACA GACTGAAGGA TGACATCCAG ACAAAGTCCG AACAGATCCA GCAGATGGCT 
GATAAAATTC TGGAGCTGGA GGAGAAACAT CGGGAGGCTC AGGTCTCAGC TCAACATCTA 
GAAGTACACT TGAAACAGAA AGAACAGCAC TACGAGGAAA AGATCAAGGT ATTGGACAAT 
CAGATAAAGA AGGACCTGGC CGACAAGGAG AGCCTGGAGA ACATGATGCA GAGACACGAG 
GAGGAGGCCC ACGAGAAGGG CAAGATCCTC AGCGAGCAGA AGGCGATGAT CAACGCGATG 
GATTCCAAGA TCCGATCCCT GGAGCAGAGG ATCGTGGAGC TGTCGGAAGC CAACAAGCTT 
GCGGCAAACA GCAGTCTCTT CACCCAGAGG AACATGAAGG CCCAGGAAGA GATGATCTCA 
GAACTCAGGC AGCAGAAATT TTACCTGGAG ACGCAGGCCG GAAAGCTGGA GGCCCAGAAC 
CGAAAGCTGG AAGAGCAACT GGAGAAGATC AGCCACCAAG ATCACAGTGA CAAGAGTCGG 
CTGCTGGAGC TGGAAACAAG GCTGAGGGAG GTCAGCCTGG AGCACGAGGA GCAGAAGCTG 
GAGCTGAAGC GGCAGCTCAC GGAGCTGCAG CTGTCCCTGC AGGAGCGCGA GTCCCAGCTG 
ACGGCCCTGC AGGCCGCCCG GGCAGCGCTG GAGAGCCAGC TCCGCCAGGC GAAGACAGAG 
CTGGAGGAGA CAACCGCGGA AGCGGAGGAG GAGATCCAGG CGCTCACGGC ACATCGAGAT 
GAGATCCAGC GCAAATTCGA TGCCCTTCGC AACAGCTGCA CGGTCATCAC CGACCTGGAG 
GAGCAGCTGA ACCAGCTCAC CGAGGACAAC GCCGAGCTCA ACAACCAAAA CTTCTACCTG 
TCCAAACAAC TCGATGAGGC TTCCGGGGCC AATGACGAGA TTGTGCAGCT GCGGAGTGAG 
GTGGACCATC TCCGCCGTGA GATCACGGAG CGGGAGATGC AGCTCACCAG CCAGAAGCAA 
GCACAACTCA GTGCTCCTGA TTTGCAGACG ATGGAGGCTC TGAAGACGAC ATGCACCATG 
CTGGAGGAGC AGGTCCTGGA CCTGGAGGCC CTGAACGACG AGCTGCTGGA GAAGGAGCGC 
CAGTGGGAGG CCTGGCGGAG CGTCCTCGGC GACGAGAAGT CCCAGTTCGA GTGTCGAGTT 
CGAGAGCTAC AGAGGATGCT GGACACCGAG AAGCAGAGCA GGGCTAGGGC CGATCAGCGG 
ATCACCGAGT CGCGCCAGGT GGTGGAGTTG GCGGTGAAGG AACACAAGGC CGAGATTCTT 
GCTCTGCAGC AGGCTCTCAA GGAGCAGAAG CTCAAAGCCG AGAGCCTGTC GGACAAGCTC 
AACGACCTGG AGAAGAAGCA CGCCATGCTG GAGATGAACG CCCGGAGCTT ACAGCAGAAA 
CTAGAGACAG AGCGGGAGCT CAAACAGAGG CTTCTGGAGG AGCAAGCCAA ATTGCAGCAG 
CAGATGGACC TGCAGAAGAA CCACATCTTC AGACTGACGC AAGGGCTGCA GGAGGCGCTG 
GACCGGGCGG ATCTGCTGAA GACAGAAAGG AGCGACCTGG AGTACCAGCT GGAAAACATT 
CAGGTTCTCT ACTCTCACGA GAAAGTGAAA ATGGAAGGCA CAATCTCTCA GCAAACCAAA 
CTCATTGATT TCCTGCAAGC CAAAATGGAC CAGCCTGCTA AAAAGAAAAA GGGTTTATTT 
AGTCGACGGA AAGAGGACCC TGCTTTGCCC ACACAGGTTC CTCTGCAGTA CAATGAGCTG 
AAGCTAGCCC TGGAAAAGGA GAAAGCCCGA TGCGCGGAGC TGGAGGAGGC CCTTCAGAAG 
ACCCGCATCG AACTCCGCTC TGCGCGGGAG GAAGCTGCCC ACCGCAAAGC CACGGACCAC 
CCGCACCCGT CTACGCCAGC CACTGCGAGG CAGCAGATCG CCATGTCTGC CATTGTGCGG 
TCGCCCGAGC ACCAGCCCAG TGCCATGAGC CTGCTTGCCC CACCATCCAG CCGCAGAAAG 
GAGTCGTCAA CTCCAGAGGA ATTCAGCCGG CGTCTGAAAG AGCGCATGCA CCACAACATC 
CCTCACCGGT TTAATGTGGG CCTGAACATG AGAGCCACCA AGTGCGCCGT GTGTCTGGAT 
ACTGTGCACT TTGGACGCCA GGCATCCAAA TGCCTCGAAT GTCAAGTGAT GTGTCACCCC 
AAATGCTCCA CCTGCTTGCC CGCTACCTGT GGCCTGCCAG CTGAATATGC CACACACTTC 
ACTGAGGCCT TCTGCCGGGA CAAAATGAAC TCCCCGGGGC TCCAGAGCAA GGAGCCTGGC 
AGCAGCTTGC ACCTGGAAGG GTGGATGAAA GTGCCCAGAA ATAACAAACG GGGACAGCAA 
GGCTGGGACA GGAAGTACAT TGTTCTGGAG GGGTCAAAAG TCCTCATCTA TGACAATGAA 
GCCAGAGAAG CTGGACAGAG GCCGGTGGAA GAATTTGAGC TGTGCCTTCC TGACGGGGAC 
GTATCTATTC ATGGTGCCGT TGGTGCTTCA GAACTTGCAA ATACCGCCAA AGCAGATGTC 
CCATACATCC TGAAGATGGA GTCTCACCCA CACACCACCT GCTGGCCTGG GAGAACCCTC 
TACTTGCTAG CACCCAGCTT CCCCGACAAG CAGCGCTGGG TCACCGCCTT AGAATCTGTC 
GTCGCAGGTG GGAGAGTTTC TAGGGAAAAG GCCGAAGCCG ATGCTGCACG TGACCATACT 
TCGAGTGAGC ATCAGCCAGT GTGGGTTGAG AAATTACTTG GAAACTCTCT GCTGAAACTG 
GAGGGCGATG ACCGGCTTGA CATGAACTGC ACCCTGCCCT TCAGTGACCA GGTAGTGCTG 
GTGGGCACGG AGGAAGGCCT CTACGCCCTG AATGTCTTGA AAAACTCCTT AACCCACATC 
CCAGGGATTG GCGCAGTCTT CCAAATTTAC ATCATCAAGG ACCTGGAGAA GCTGCTCATG 
ATAGCAGGGG AAGAGCGGGC TCTGTGCCTG GTGGACGTGA AGAAGGTGAA GCAGTCCCTG 
GCGCAGTCAC ACCTGCCTGC CCAGCCCGAC GTCTCCCCCA ACATATTCGA AGCCGTCAAA 
GGCTGCCACT TGTTCGCTGC TGGCAAGATC GAGAACAGCC TGTGCATCTG CGCCGCTATG 
CCAAGCAAAG TCGTCATCCT CCGCTACAAT GACAACCTCA GCAAGTACTG CATCCGCAAG 
GAGATCGAGA CCTCAGAGCC CTGCAGCTGT ATCCACTTCA CCAACTACAG CATCCTCATT 
GGGACCAACA AATTCTATGA GATCGACATG AAGCAGTACA CGCTTGACGA GTTCCTGGAC 
AAGAACGACC ATTCCTTGGC ACCTGCTGTG TTCGCCTCCT CGTCCAACAG CTTCCCTGTC 
TCCATTGTGC AGGCGAACAG CGCCGGGCAG CGAGAAGAAT ACCTGCTGTG CTTCCACGAA 
TTTGGGGTGT TCGTGGATTC TTACGGAAGA CGTAGCCGCA CAGATGATCT CAAGTGGAGT 
CGCTTACCTC TGGCCTTCGC CTACAGAGAA CCTTATCTGT TTGTGACTCA CTTCAACTCC 
CTGGAAGTCA TTGAGATCCA GGCCAGATCC TCACTGGGGA GCCCTGCCCG AGCATATCTG 
GAAATTCCAA ACCCTCGCTA CCTGGGCCCC GCGATTTCCT CCGGAGCGAT TTACCTGGCC 
TCCTCATACC AGGACAAGTT AAGGGTCATA TGCTGCAAAG GAAACCTCGT GAAGGAGTCA 
GGCACTGAGC AGCACCGGGT GCCCTCCACC TCCCGCAGCA GCCCCAACAA GCGAGGCCCA 
CCAACATACA ACGAGCACAT CACCAAACGC GTGGCCTCCA GCCCGGCGCC ACCGGAAGGC 
CCCAGCCACC CCCGAGAGCC AAGCACACCG CACCGCTACC GAGACAGAGA GGGTCGGACA 
GAGCTGCGCA GGGACAAGTC TCCAGGCCGC CCTCTGGAGC GGGAGAAGTC CCCAGGCCGA 
ATGCTCAGCA CTAGGAGAGA GCGGTCCCCA GGGAGACTGT TTGAAGACAG CAGCAGGGGC 
CGGCTGCCTG CAGGAGCAGT GAGGACCCCA CTGTCCCAGG TTAACAAGGT CTGGGACCAG 
TCTTCAGTAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006530193.1
CDS	158..6529
Translation	MLKFKYGVRNPPEASASEPIASRASRLNLFFQGKPPLMTQQQMSALSREGMLDALFALFEECSQPALMKMKHVSSFVQKYSDTIAELRELQPSARDFEVRSLVGCGHFAEVQVVREKATGDVYAMKIMKKKALLAQEQVSFFEEERNILSRSTSPWIPQLQYAFQDKNNLYLVMEYQPGGDLLSLLNRYEDQLDESMIQFYLAELILAVHSVHQMGYVHRDIKPENILIDRTGHIKLVDFGSAAKMNSNKVDAKLPIGTPDYMAPEVLTVMNEDRRGTYGLDCDWWSVGVVAYEMVYGKTPFTEGTSARTFNNIMNFQRFLKFPDDPKVSSELLDLLQSLLCVQKERLKFEGLCCHPFFARTDWNNIRNSPPPFVPTLKSDDDTSNFDEPEKNSWVSSSVCQLSPSGFSGEELPFVGFSYSKALGYLGRSESVVSSLDSPAKVSSMEKKLLIKSKELQDSQDKCHKMEQEMTRLHRRVSEVEAVLSQKEVELKASETQRSLLEQDLATYITECSSLKRSLEQARMEVSQEDDKALQLLHDIREQSRKLQEIKEQEYQAQVEEMRLMMNQLEEDLVSARRRSDLYESELRESRLAAEEFKRKANECQHKLMKVVSHPPRGDSGGTALDDLHKMQGHAGLTSAKDQGKPEVGEYSKLEKINAEQQLKIQELQEKLEKAVKASTEATELLQNIRQAKERAERELEKLHNREDSSEGIKKKLVEAEERRHSLENKVKRLETMERRENRLKDDIQTKSEQIQQMADKILELEEKHREAQVSAQHLEVHLKQKEQHYEEKIKVLDNQIKKDLADKESLENMMQRHEEEAHEKGKILSEQKAMINAMDSKIRSLEQRIVELSEANKLAANSSLFTQRNMKAQEEMISELRQQKFYLETQAGKLEAQNRKLEEQLEKISHQDHSDKSRLLELETRLREVSLEHEEQKLELKRQLTELQLSLQERESQLTALQAARAALESQLRQAKTELEETTAEAEEEIQALTAHRDEIQRKFDALRNSCTVITDLEEQLNQLTEDNAELNNQNFYLSKQLDEASGANDEIVQLRSEVDHLRREITEREMQLTSQKQAQLSAPDLQTMEALKTTCTMLEEQVLDLEALNDELLEKERQWEAWRSVLGDEKSQFECRVRELQRMLDTEKQSRARADQRITESRQVVELAVKEHKAEILALQQALKEQKLKAESLSDKLNDLEKKHAMLEMNARSLQQKLETERELKQRLLEEQAKLQQQMDLQKNHIFRLTQGLQEALDRADLLKTERSDLEYQLENIQVLYSHEKVKMEGTISQQTKLIDFLQAKMDQPAKKKKGLFSRRKEDPALPTQVPLQYNELKLALEKEKARCAELEEALQKTRIELRSAREEAAHRKATDHPHPSTPATARQQIAMSAIVRSPEHQPSAMSLLAPPSSRRKESSTPEEFSRRLKERMHHNIPHRFNVGLNMRATKCAVCLDTVHFGRQASKCLECQVMCHPKCSTCLPATCGLPAEYATHFTEAFCRDKMNSPGLQSKEPGSSLHLEGWMKVPRNNKRGQQGWDRKYIVLEGSKVLIYDNEAREAGQRPVEEFELCLPDGDVSIHGAVGASELANTAKADVPYILKMESHPHTTCWPGRTLYLLAPSFPDKQRWVTALESVVAGGRVSREKAEADAARDHTSSEHQPVWVEKLLGNSLLKLEGDDRLDMNCTLPFSDQVVLVGTEEGLYALNVLKNSLTHIPGIGAVFQIYIIKDLEKLLMIAGEERALCLVDVKKVKQSLAQSHLPAQPDVSPNIFEAVKGCHLFAAGKIENSLCICAAMPSKVVILRYNDNLSKYCIRKEIETSEPCSCIHFTNYSILIGTNKFYEIDMKQYTLDEFLDKNDHSLAPAVFASSSNSFPVSIVQANSAGQREEYLLCFHEFGVFVDSYGRRSRTDDLKWSRLPLAFAYREPYLFVTHFNSLEVIEIQARSSLGSPARAYLEIPNPRYLGPAISSGAIYLASSYQDKLRVICCKGNLVKESGTEQHRVPSTSRSSPNKRGPPTYNEHITKRVASSPAPPEGPSHPREPSTPHRYRDREGRTELRRDKSPGRPLEREKSPGRMLSTRRERSPGRLFEDSSRGRLPAGAVRTPLSQVNKVWDQSSV

Target ORF information:

RefSeq Version	XM_006530130.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus citron (Cit), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006530130.4

ORF Insert Sequence:

ATGTTGAAGT TCAAGTATGG TGTGCGGAAC CCGCCGGAGG CCAGTGCCTC CGAGCCCATT 
GCCAGTCGGG CCTCCAGGCT AAATCTCTTC TTCCAGGGGA AACCGCCCCT CATGACTCAA 
CAGCAGATGT CTGCTCTTTC CCGGGAAGGG ATGCTAGACG CCCTCTTCGC TCTCTTTGAA 
GAGTGCAGCC AACCCGCCCT GATGAAGATG AAGCACGTGA GCAGCTTTGT CCAGAAGTAT 
TCCGACACCA TAGCCGAGTT GCGGGAGCTG CAGCCGTCGG CGAGAGACTT CGAAGTTCGA 
AGCCTTGTGG GCTGTGGTCA CTTCGCTGAA GTGCAGGTGG TTAGAGAGAA GGCGACCGGG 
GACGTCTATG CCATGAAAAT CATGAAGAAG AAGGCTTTGC TGGCCCAGGA ACAGGTTTCA 
TTTTTCGAGG AGGAGAGGAA CATATTATCT CGGAGCACGA GTCCTTGGAT CCCCCAGTTA 
CAGTACGCCT TTCAGGACAA AAATAACCTT TACCTGGTCA TGGAATATCA GCCTGGAGGG 
GATTTGCTGT CGCTTCTGAA CAGATACGAG GACCAATTAG ATGAGAGCAT GATCCAGTTT 
TACCTTGCTG AGCTGATTTT GGCTGTCCAC AGCGTGCACC AGATGGGATA TGTGCATCGA 
GACATCAAGC CCGAGAACAT CCTCATCGAC CGGACGGGAC ACATCAAGCT GGTGGATTTT 
GGATCAGCCG CTAAGATGAA TTCAAATAAG GTGGATGCCA AACTCCCCAT TGGGACCCCG 
GATTACATGG CTCCGGAAGT GTTGACCGTG ATGAACGAGG ACCGAAGGGG CACATACGGC 
TTGGACTGTG ACTGGTGGTC TGTCGGAGTT GTTGCTTATG AGATGGTTTA TGGGAAGACC 
CCATTCACAG AGGGAACCTC CGCCCGGACC TTCAACAACA TCATGAACTT CCAGCGGTTT 
TTGAAGTTCC CAGATGACCC CAAAGTTAGC AGTGAGCTCC TTGATCTGCT TCAGAGTCTG 
CTGTGTGTCC AGAAAGAGAG ACTGAAGTTC GAGGGTCTCT GCTGCCACCC TTTCTTTGCC 
AGAACGGACT GGAACAACAT CCGTAACTCT CCTCCCCCCT TCGTCCCCAC CCTCAAGTCT 
GACGATGACA CCTCCAATTT TGATGAACCA GAGAAGAATT CGTGGGTTTC ATCCTCTGTG 
TGCCAGCTGA GCCCCTCGGG CTTCTCAGGC GAAGAGCTGC CGTTTGTGGG ATTTTCGTAC 
AGCAAGGCAC TGGGGTATCT TGGTAGATCT GAGTCTGTCG TGTCGAGTCT GGACTCCCCT 
GCCAAGGTTA GCTCCATGGA AAAGAAACTT CTCATCAAAA GCAAAGAGCT CCAAGACTCC 
CAGGACAAGT GTCACAAGAT GGAGCAGGAA ATGACCCGGT TACATCGCAG AGTGTCAGAG 
GTGGAGGCTG TGCTTAGTCA GAAGGAGGTG GAGCTGAAGG CCTCTGAGAC TCAGAGATCC 
CTCCTGGAGC AGGACCTTGC TACCTACATC ACAGAATGCA GTAGCTTAAA GCGAAGTTTG 
GAGCAAGCGC GGATGGAGGT GTCCCAGGAG GATGACAAAG CTCTGCAGCT TCTCCACGAC 
ATCCGAGAGC AGAGCCGGAA GCTCCAGGAG ATCAAGGAGC AGGAGTACCA GGCTCAGGTG 
GAGGAGATGA GGCTGATGAT GAATCAGCTG GAAGAAGACC TGGTGTCAGC CCGCAGACGC 
AGCGATCTCT ACGAGTCTGA GCTGAGGGAG TCTCGGCTTG CCGCCGAGGA ATTCAAGCGG 
AAGGCAAACG AATGTCAGCA CAAACTGATG AAGGTAGTCA GCCACCCTCC AAGAGGGGAC 
TCCGGGGGCA CGGCCCTGGA TGATCTTCAC AAGATGCAAG GCCACGCTGG CTTAACGAGT 
GCTAAGGACC AAGGGAAGCC TGAAGTGGGA GAATATTCCA AACTGGAGAA GATCAATGCT 
GAGCAGCAGC TGAAGATCCA GGAGCTCCAG GAGAAGCTGG AGAAGGCTGT AAAAGCCAGC 
ACAGAGGCCA CCGAGCTCCT GCAGAATATC CGCCAGGCAA AGGAGCGAGC AGAGCGGGAG 
CTAGAGAAGC TACACAACCG GGAAGACTCC TCCGAGGGCA TCAAAAAGAA GCTGGTGGAA 
GCCGAGGAAC GCCGCCACTC CCTGGAGAAC AAGGTAAAGA GACTAGAGAC CATGGAGCGT 
AGAGAGAACA GACTGAAGGA TGACATCCAG ACAAAGTCCG AACAGATCCA GCAGATGGCT 
GATAAAATTC TGGAGCTGGA GGAGAAACAT CGGGAGGCTC AGGTCTCAGC TCAACATCTA 
GAAGTACACT TGAAACAGAA AGAACAGCAC TACGAGGAAA AGATCAAGGT ATTGGACAAT 
CAGATAAAGA AGGACCTGGC CGACAAGGAG AGCCTGGAGA ACATGATGCA GAGACACGAG 
GAGGAGGCCC ACGAGAAGGG CAAGATCCTC AGCGAGCAGA AGGCGATGAT CAACGCGATG 
GATTCCAAGA TCCGATCCCT GGAGCAGAGG ATCGTGGAGC TGTCGGAAGC CAACAAGCTT 
GCGGCAAACA GCAGTCTCTT CACCCAGAGG AACATGAAGG CCCAGGAAGA GATGATCTCA 
GAACTCAGGC AGCAGAAATT TTACCTGGAG ACGCAGGCCG GAAAGCTGGA GGCCCAGAAC 
CGAAAGCTGG AAGAGCAACT GGAGAAGATC AGCCACCAAG ATCACAGTGA CAAGAGTCGG 
CTGCTGGAGC TGGAAACAAG GCTGAGGGAG GTCAGCCTGG AGCACGAGGA GCAGAAGCTG 
GAGCTGAAGC GGCAGCTCAC GGAGCTGCAG CTGTCCCTGC AGGAGCGCGA GTCCCAGCTG 
ACGGCCCTGC AGGCCGCCCG GGCAGCGCTG GAGAGCCAGC TCCGCCAGGC GAAGACAGAG 
CTGGAGGAGA CAACCGCGGA AGCGGAGGAG GAGATCCAGG CGCTCACGGC ACATCGAGAT 
GAGATCCAGC GCAAATTCGA TGCCCTTCGC AACAGCTGCA CGGTCATCAC CGACCTGGAG 
GAGCAGCTGA ACCAGCTCAC CGAGGACAAC GCCGAGCTCA ACAACCAAAA CTTCTACCTG 
TCCAAACAAC TCGATGAGGC TTCCGGGGCC AATGACGAGA TTGTGCAGCT GCGGAGTGAG 
GTGGACCATC TCCGCCGTGA GATCACGGAG CGGGAGATGC AGCTCACCAG CCAGAAGCAA 
GCACAACTCA GTGCTCCTGA TTTGCAGACG ATGGAGGCTC TGAAGACGAC ATGCACCATG 
CTGGAGGAGC AGGTCCTGGA CCTGGAGGCC CTGAACGACG AGCTGCTGGA GAAGGAGCGC 
CAGTGGGAGG CCTGGCGGAG CGTCCTCGGC GACGAGAAGT CCCAGTTCGA GTGTCGAGTT 
CGAGAGCTAC AGAGGATGCT GGACACCGAG AAGCAGAGCA GGGCTAGGGC CGATCAGCGG 
ATCACCGAGT CGCGCCAGGT GGTGGAGTTG GCGGTGAAGG AACACAAGGC CGAGATTCTT 
GCTCTGCAGC AGGCTCTCAA GGAGCAGAAG CTCAAAGCCG AGAGCCTGTC GGACAAGCTC 
AACGACCTGG AGAAGAAGCA CGCCATGCTG GAGATGAACG CCCGGAGCTT ACAGCAGAAA 
CTAGAGACAG AGCGGGAGCT CAAACAGAGG CTTCTGGAGG AGCAAGCCAA ATTGCAGCAG 
CAGATGGACC TGCAGAAGAA CCACATCTTC AGACTGACGC AAGGGCTGCA GGAGGCGCTG 
GACCGGGCGG ATCTGCTGAA GACAGAAAGG AGCGACCTGG AGTACCAGCT GGAAAACATT 
CAGGTTCTCT ACTCTCACGA GAAAGTGAAA ATGGAAGGCA CAATCTCTCA GCAAACCAAA 
CTCATTGATT TCCTGCAAGC CAAAATGGAC CAGCCTGCTA AAAAGAAAAA GGGTTTATTT 
AGTCGACGGA AAGAGGACCC TGCTTTGCCC ACACAGGTTC CTCTGCAGTA CAATGAGCTG 
AAGCTAGCCC TGGAAAAGGA GAAAGCCCGA TGCGCGGAGC TGGAGGAGGC CCTTCAGAAG 
ACCCGCATCG AACTCCGCTC TGCGCGGGAG GAAGCTGCCC ACCGCAAAGC CACGGACCAC 
CCGCACCCGT CTACGCCAGC CACTGCGAGG CAGCAGATCG CCATGTCTGC CATTGTGCGG 
TCGCCCGAGC ACCAGCCCAG TGCCATGAGC CTGCTTGCCC CACCATCCAG CCGCAGAAAG 
GAGTCGTCAA CTCCAGAGGA ATTCAGCCGG CGTCTGAAAG AGCGCATGCA CCACAACATC 
CCTCACCGGT TTAATGTGGG CCTGAACATG AGAGCCACCA AGTGCGCCGT GTGTCTGGAT 
ACTGTGCACT TTGGACGCCA GGCATCCAAA TGCCTCGAAT GTCAAGTGAT GTGTCACCCC 
AAATGCTCCA CCTGCTTGCC CGCTACCTGT GGCCTGCCAG CTGAATATGC CACACACTTC 
ACTGAGGCCT TCTGCCGGGA CAAAATGAAC TCCCCGGGGC TCCAGAGCAA GGAGCCTGGC 
AGCAGCTTGC ACCTGGAAGG GTGGATGAAA GTGCCCAGAA ATAACAAACG GGGACAGCAA 
GGCTGGGACA GGAAGTACAT TGTTCTGGAG GGGTCAAAAG TCCTCATCTA TGACAATGAA 
GCCAGAGAAG CTGGACAGAG GCCGGTGGAA GAATTTGAGC TGTGCCTTCC TGACGGGGAC 
GTATCTATTC ATGGTGCCGT TGGTGCTTCA GAACTTGCAA ATACCGCCAA AGCAGATGTC 
CCATACATCC TGAAGATGGA GTCTCACCCA CACACCACCT GCTGGCCTGG GAGAACCCTC 
TACTTGCTAG CACCCAGCTT CCCCGACAAG CAGCGCTGGG TCACCGCCTT AGAATCTGTC 
GTCGCAGGTG GGAGAGTTTC TAGGGAAAAG GCCGAAGCCG ATGCTGCACG TGACCATACT 
TCGAGTGAGC ATCAGCCAGT GTGGGTTGAG AAATTACTTG GAAACTCTCT GCTGAAACTG 
GAGGGCGATG ACCGGCTTGA CATGAACTGC ACCCTGCCCT TCAGTGACCA GGTAGTGCTG 
GTGGGCACGG AGGAAGGCCT CTACGCCCTG AATGTCTTGA AAAACTCCTT AACCCACATC 
CCAGGGATTG GCGCAGTCTT CCAAATTTAC ATCATCAAGG ACCTGGAGAA GCTGCTCATG 
ATAGCAGGGG AAGAGCGGGC TCTGTGCCTG GTGGACGTGA AGAAGGTGAA GCAGTCCCTG 
GCGCAGTCAC ACCTGCCTGC CCAGCCCGAC GTCTCCCCCA ACATATTCGA AGCCGTCAAA 
GGCTGCCACT TGTTCGCTGC TGGCAAGATC GAGAACAGCC TGTGCATCTG CGCCGCTATG 
CCAAGCAAAG TCGTCATCCT CCGCTACAAT GACAACCTCA GCAAGTACTG CATCCGCAAG 
GAGATCGAGA CCTCAGAGCC CTGCAGCTGT ATCCACTTCA CCAACTACAG CATCCTCATT 
GGGACCAACA AATTCTATGA GATCGACATG AAGCAGTACA CGCTTGACGA GTTCCTGGAC 
AAGAACGACC ATTCCTTGGC ACCTGCTGTG TTCGCCTCCT CGTCCAACAG CTTCCCTGTC 
TCCATTGTGC AGGCGAACAG CGCCGGGCAG CGAGAAGAAT ACCTGCTGTG CTTCCACGAA 
TTTGGGGTGT TCGTGGATTC TTACGGAAGA CGTAGCCGCA CAGATGATCT CAAGTGGAGT 
CGCTTACCTC TGGCCTTCGC CTACAGAGAA CCTTATCTGT TTGTGACTCA CTTCAACTCC 
CTGGAAGTCA TTGAGATCCA GGCCAGATCC TCACTGGGGA GCCCTGCCCG AGCATATCTG 
GAAATTCCAA ACCCTCGCTA CCTGGGCCCC GCGATTTCCT CCGGAGCGAT TTACCTGGCC 
TCCTCATACC AGGACAAGTT AAGGGTCATA TGCTGCAAAG GAAACCTCGT GAAGGAGTCA 
GGCACTGAGC AGCACCGGGT GCCCTCCACC TCCCGCAGCA GCCCCAACAA GCGAGGCCCA 
CCAACATACA ACGAGCACAT CACCAAACGC GTGGCCTCCA GCCCGGCGCC ACCGGAAGGC 
CCCAGCCACC CCCGAGAGCC AAGCACACCG CACCGCTACC GAGACAGAGA GGGTCGGACA 
GAGCTGCGCA GGGACAAGTC TCCAGGCCGC CCTCTGGAGC GGGAGAAGTC CCCAGGCCGA 
ATGCTCAGCA CTAGGAGAGA GCGGTCCCCA GGGAGACTGT TTGAAGACAG CAGCAGGGGC 
CGGCTGCCTG CAGGAGCAGT GAGGACCCCA CTGTCCCAGG TTAACAAGGT CTGGGACCAG 
TCTTCAGTAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cit ( XM_006530131.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006530194.1 Mus musculus citron (Cit), transcript variant X2, mRNA.

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CloneID	OMu35774
Clone ID Related Accession (Same CDS sequence)	XM_006530130.3 , XM_006530132.3 , XM_006530133.3 , XM_006530130.4 , XM_006530131.2 , XM_006530131.1 , XM_006530133.4 , XM_006530132.4
Accession Version	XM_006530131.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6372bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	citron Rho-interacting kinase isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000071.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006530131.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTGAAGT TCAAGTATGG TGTGCGGAAC CCGCCGGAGG CCAGTGCCTC CGAGCCCATT 
GCCAGTCGGG CCTCCAGGCT AAATCTCTTC TTCCAGGGGA AACCGCCCCT CATGACTCAA 
CAGCAGATGT CTGCTCTTTC CCGGGAAGGG ATGCTAGACG CCCTCTTCGC TCTCTTTGAA 
GAGTGCAGCC AACCCGCCCT GATGAAGATG AAGCACGTGA GCAGCTTTGT CCAGAAGTAT 
TCCGACACCA TAGCCGAGTT GCGGGAGCTG CAGCCGTCGG CGAGAGACTT CGAAGTTCGA 
AGCCTTGTGG GCTGTGGTCA CTTCGCTGAA GTGCAGGTGG TTAGAGAGAA GGCGACCGGG 
GACGTCTATG CCATGAAAAT CATGAAGAAG AAGGCTTTGC TGGCCCAGGA ACAGGTTTCA 
TTTTTCGAGG AGGAGAGGAA CATATTATCT CGGAGCACGA GTCCTTGGAT CCCCCAGTTA 
CAGTACGCCT TTCAGGACAA AAATAACCTT TACCTGGTCA TGGAATATCA GCCTGGAGGG 
GATTTGCTGT CGCTTCTGAA CAGATACGAG GACCAATTAG ATGAGAGCAT GATCCAGTTT 
TACCTTGCTG AGCTGATTTT GGCTGTCCAC AGCGTGCACC AGATGGGATA TGTGCATCGA 
GACATCAAGC CCGAGAACAT CCTCATCGAC CGGACGGGAC ACATCAAGCT GGTGGATTTT 
GGATCAGCCG CTAAGATGAA TTCAAATAAG GTGGATGCCA AACTCCCCAT TGGGACCCCG 
GATTACATGG CTCCGGAAGT GTTGACCGTG ATGAACGAGG ACCGAAGGGG CACATACGGC 
TTGGACTGTG ACTGGTGGTC TGTCGGAGTT GTTGCTTATG AGATGGTTTA TGGGAAGACC 
CCATTCACAG AGGGAACCTC CGCCCGGACC TTCAACAACA TCATGAACTT CCAGCGGTTT 
TTGAAGTTCC CAGATGACCC CAAAGTTAGC AGTGAGCTCC TTGATCTGCT TCAGAGTCTG 
CTGTGTGTCC AGAAAGAGAG ACTGAAGTTC GAGGGTCTCT GCTGCCACCC TTTCTTTGCC 
AGAACGGACT GGAACAACAT CCGTAACTCT CCTCCCCCCT TCGTCCCCAC CCTCAAGTCT 
GACGATGACA CCTCCAATTT TGATGAACCA GAGAAGAATT CGTGGGTTTC ATCCTCTGTG 
TGCCAGCTGA GCCCCTCGGG CTTCTCAGGC GAAGAGCTGC CGTTTGTGGG ATTTTCGTAC 
AGCAAGGCAC TGGGGTATCT TGGTAGATCT GAGTCTGTCG TGTCGAGTCT GGACTCCCCT 
GCCAAGGTTA GCTCCATGGA AAAGAAACTT CTCATCAAAA GCAAAGAGCT CCAAGACTCC 
CAGGACAAGT GTCACAAGAT GGAGCAGGAA ATGACCCGGT TACATCGCAG AGTGTCAGAG 
GTGGAGGCTG TGCTTAGTCA GAAGGAGGTG GAGCTGAAGG CCTCTGAGAC TCAGAGATCC 
CTCCTGGAGC AGGACCTTGC TACCTACATC ACAGAATGCA GTAGCTTAAA GCGAAGTTTG 
GAGCAAGCGC GGATGGAGGT GTCCCAGGAG GATGACAAAG CTCTGCAGCT TCTCCACGAC 
ATCCGAGAGC AGAGCCGGAA GCTCCAGGAG ATCAAGGAGC AGGAGTACCA GGCTCAGGTG 
GAGGAGATGA GGCTGATGAT GAATCAGCTG GAAGAAGACC TGGTGTCAGC CCGCAGACGC 
AGCGATCTCT ACGAGTCTGA GCTGAGGGAG TCTCGGCTTG CCGCCGAGGA ATTCAAGCGG 
AAGGCAAACG AATGTCAGCA CAAACTGATG AAGGTAGTCA GCCACCCTCC AAGAGGGGAC 
TCCGGGGGCA CGGCCCTGGA TGATCTTCAC AAGATGCAAG GCCACGCTGG CTTAACGAGT 
GCTAAGGACC AAGGGAAGCC TGAAGTGGGA GAATATTCCA AACTGGAGAA GATCAATGCT 
GAGCAGCAGC TGAAGATCCA GGAGCTCCAG GAGAAGCTGG AGAAGGCTGT AAAAGCCAGC 
ACAGAGGCCA CCGAGCTCCT GCAGAATATC CGCCAGGCAA AGGAGCGAGC AGAGCGGGAG 
CTAGAGAAGC TACACAACCG GGAAGACTCC TCCGAGGGCA TCAAAAAGAA GCTGGTGGAA 
GCCGAGGAAC GCCGCCACTC CCTGGAGAAC AAGGTAAAGA GACTAGAGAC CATGGAGCGT 
AGAGAGAACA GACTGAAGGA TGACATCCAG ACAAAGTCCG AACAGATCCA GCAGATGGCT 
GATAAAATTC TGGAGCTGGA GGAGAAACAT CGGGAGGCTC AGGTCTCAGC TCAACATCTA 
GAAGTACACT TGAAACAGAA AGAACAGCAC TACGAGGAAA AGATCAAGGT ATTGGACAAT 
CAGATAAAGA AGGACCTGGC CGACAAGGAG AGCCTGGAGA ACATGATGCA GAGACACGAG 
GAGGAGGCCC ACGAGAAGGG CAAGATCCTC AGCGAGCAGA AGGCGATGAT CAACGCGATG 
GATTCCAAGA TCCGATCCCT GGAGCAGAGG ATCGTGGAGC TGTCGGAAGC CAACAAGCTT 
GCGGCAAACA GCAGTCTCTT CACCCAGAGG AACATGAAGG CCCAGGAAGA GATGATCTCA 
GAACTCAGGC AGCAGAAATT TTACCTGGAG ACGCAGGCCG GAAAGCTGGA GGCCCAGAAC 
CGAAAGCTGG AAGAGCAACT GGAGAAGATC AGCCACCAAG ATCACAGTGA CAAGAGTCGG 
CTGCTGGAGC TGGAAACAAG GCTGAGGGAG GTCAGCCTGG AGCACGAGGA GCAGAAGCTG 
GAGCTGAAGC GGCAGCTCAC GGAGCTGCAG CTGTCCCTGC AGGAGCGCGA GTCCCAGCTG 
ACGGCCCTGC AGGCCGCCCG GGCAGCGCTG GAGAGCCAGC TCCGCCAGGC GAAGACAGAG 
CTGGAGGAGA CAACCGCGGA AGCGGAGGAG GAGATCCAGG CGCTCACGGC ACATCGAGAT 
GAGATCCAGC GCAAATTCGA TGCCCTTCGC AACAGCTGCA CGGTCATCAC CGACCTGGAG 
GAGCAGCTGA ACCAGCTCAC CGAGGACAAC GCCGAGCTCA ACAACCAAAA CTTCTACCTG 
TCCAAACAAC TCGATGAGGC TTCCGGGGCC AATGACGAGA TTGTGCAGCT GCGGAGTGAG 
GTGGACCATC TCCGCCGTGA GATCACGGAG CGGGAGATGC AGCTCACCAG CCAGAAGCAA 
GCACAACTCA GTGCTCCTGA TTTGCAGACG ATGGAGGCTC TGAAGACGAC ATGCACCATG 
CTGGAGGAGC AGGTCCTGGA CCTGGAGGCC CTGAACGACG AGCTGCTGGA GAAGGAGCGC 
CAGTGGGAGG CCTGGCGGAG CGTCCTCGGC GACGAGAAGT CCCAGTTCGA GTGTCGAGTT 
CGAGAGCTAC AGAGGATGCT GGACACCGAG AAGCAGAGCA GGGCTAGGGC CGATCAGCGG 
ATCACCGAGT CGCGCCAGGT GGTGGAGTTG GCGGTGAAGG AACACAAGGC CGAGATTCTT 
GCTCTGCAGC AGGCTCTCAA GGAGCAGAAG CTCAAAGCCG AGAGCCTGTC GGACAAGCTC 
AACGACCTGG AGAAGAAGCA CGCCATGCTG GAGATGAACG CCCGGAGCTT ACAGCAGAAA 
CTAGAGACAG AGCGGGAGCT CAAACAGAGG CTTCTGGAGG AGCAAGCCAA ATTGCAGCAG 
CAGATGGACC TGCAGAAGAA CCACATCTTC AGACTGACGC AAGGGCTGCA GGAGGCGCTG 
GACCGGGCGG ATCTGCTGAA GACAGAAAGG AGCGACCTGG AGTACCAGCT GGAAAACATT 
CAGGTTCTCT ACTCTCACGA GAAAGTGAAA ATGGAAGGCA CAATCTCTCA GCAAACCAAA 
CTCATTGATT TCCTGCAAGC CAAAATGGAC CAGCCTGCTA AAAAGAAAAA GGGTTTATTT 
AGTCGACGGA AAGAGGACCC TGCTTTGCCC ACACAGGTTC CTCTGCAGTA CAATGAGCTG 
AAGCTAGCCC TGGAAAAGGA GAAAGCCCGA TGCGCGGAGC TGGAGGAGGC CCTTCAGAAG 
ACCCGCATCG AACTCCGCTC TGCGCGGGAG GAAGCTGCCC ACCGCAAAGC CACGGACCAC 
CCGCACCCGT CTACGCCAGC CACTGCGAGG CAGCAGATCG CCATGTCTGC CATTGTGCGG 
TCGCCCGAGC ACCAGCCCAG TGCCATGAGC CTGCTTGCCC CACCATCCAG CCGCAGAAAG 
GAGTCGTCAA CTCCAGAGGA ATTCAGCCGG CGTCTGAAAG AGCGCATGCA CCACAACATC 
CCTCACCGGT TTAATGTGGG CCTGAACATG AGAGCCACCA AGTGCGCCGT GTGTCTGGAT 
ACTGTGCACT TTGGACGCCA GGCATCCAAA TGCCTCGAAT GTCAAGTGAT GTGTCACCCC 
AAATGCTCCA CCTGCTTGCC CGCTACCTGT GGCCTGCCAG CTGAATATGC CACACACTTC 
ACTGAGGCCT TCTGCCGGGA CAAAATGAAC TCCCCGGGGC TCCAGAGCAA GGAGCCTGGC 
AGCAGCTTGC ACCTGGAAGG GTGGATGAAA GTGCCCAGAA ATAACAAACG GGGACAGCAA 
GGCTGGGACA GGAAGTACAT TGTTCTGGAG GGGTCAAAAG TCCTCATCTA TGACAATGAA 
GCCAGAGAAG CTGGACAGAG GCCGGTGGAA GAATTTGAGC TGTGCCTTCC TGACGGGGAC 
GTATCTATTC ATGGTGCCGT TGGTGCTTCA GAACTTGCAA ATACCGCCAA AGCAGATGTC 
CCATACATCC TGAAGATGGA GTCTCACCCA CACACCACCT GCTGGCCTGG GAGAACCCTC 
TACTTGCTAG CACCCAGCTT CCCCGACAAG CAGCGCTGGG TCACCGCCTT AGAATCTGTC 
GTCGCAGGTG GGAGAGTTTC TAGGGAAAAG GCCGAAGCCG ATGCTGCACG TGACCATACT 
TCGAGTGAGC ATCAGCCAGT GTGGGTTGAG AAATTACTTG GAAACTCTCT GCTGAAACTG 
GAGGGCGATG ACCGGCTTGA CATGAACTGC ACCCTGCCCT TCAGTGACCA GGTAGTGCTG 
GTGGGCACGG AGGAAGGCCT CTACGCCCTG AATGTCTTGA AAAACTCCTT AACCCACATC 
CCAGGGATTG GCGCAGTCTT CCAAATTTAC ATCATCAAGG ACCTGGAGAA GCTGCTCATG 
ATAGCAGGGG AAGAGCGGGC TCTGTGCCTG GTGGACGTGA AGAAGGTGAA GCAGTCCCTG 
GCGCAGTCAC ACCTGCCTGC CCAGCCCGAC GTCTCCCCCA ACATATTCGA AGCCGTCAAA 
GGCTGCCACT TGTTCGCTGC TGGCAAGATC GAGAACAGCC TGTGCATCTG CGCCGCTATG 
CCAAGCAAAG TCGTCATCCT CCGCTACAAT GACAACCTCA GCAAGTACTG CATCCGCAAG 
GAGATCGAGA CCTCAGAGCC CTGCAGCTGT ATCCACTTCA CCAACTACAG CATCCTCATT 
GGGACCAACA AATTCTATGA GATCGACATG AAGCAGTACA CGCTTGACGA GTTCCTGGAC 
AAGAACGACC ATTCCTTGGC ACCTGCTGTG TTCGCCTCCT CGTCCAACAG CTTCCCTGTC 
TCCATTGTGC AGGCGAACAG CGCCGGGCAG CGAGAAGAAT ACCTGCTGTG CTTCCACGAA 
TTTGGGGTGT TCGTGGATTC TTACGGAAGA CGTAGCCGCA CAGATGATCT CAAGTGGAGT 
CGCTTACCTC TGGCCTTCGC CTACAGAGAA CCTTATCTGT TTGTGACTCA CTTCAACTCC 
CTGGAAGTCA TTGAGATCCA GGCCAGATCC TCACTGGGGA GCCCTGCCCG AGCATATCTG 
GAAATTCCAA ACCCTCGCTA CCTGGGCCCC GCGATTTCCT CCGGAGCGAT TTACCTGGCC 
TCCTCATACC AGGACAAGTT AAGGGTCATA TGCTGCAAAG GAAACCTCGT GAAGGAGTCA 
GGCACTGAGC AGCACCGGGT GCCCTCCACC TCCCGCAGCA GCCCCAACAA GCGAGGCCCA 
CCAACATACA ACGAGCACAT CACCAAACGC GTGGCCTCCA GCCCGGCGCC ACCGGAAGGC 
CCCAGCCACC CCCGAGAGCC AAGCACACCG CACCGCTACC GAGACAGAGA GGGTCGGACA 
GAGCTGCGCA GGGACAAGTC TCCAGGCCGC CCTCTGGAGC GGGAGAAGTC CCCAGGCCGA 
ATGCTCAGCA CTAGGAGAGA GCGGTCCCCA GGGAGACTGT TTGAAGACAG CAGCAGGGGC 
CGGCTGCCTG CAGGAGCAGT GAGGACCCCA CTGTCCCAGG TTAACAAGGT CTGGGACCAG 
TCTTCAGTAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006530194.1
CDS	103..6474
Translation	MLKFKYGVRNPPEASASEPIASRASRLNLFFQGKPPLMTQQQMSALSREGMLDALFALFEECSQPALMKMKHVSSFVQKYSDTIAELRELQPSARDFEVRSLVGCGHFAEVQVVREKATGDVYAMKIMKKKALLAQEQVSFFEEERNILSRSTSPWIPQLQYAFQDKNNLYLVMEYQPGGDLLSLLNRYEDQLDESMIQFYLAELILAVHSVHQMGYVHRDIKPENILIDRTGHIKLVDFGSAAKMNSNKVDAKLPIGTPDYMAPEVLTVMNEDRRGTYGLDCDWWSVGVVAYEMVYGKTPFTEGTSARTFNNIMNFQRFLKFPDDPKVSSELLDLLQSLLCVQKERLKFEGLCCHPFFARTDWNNIRNSPPPFVPTLKSDDDTSNFDEPEKNSWVSSSVCQLSPSGFSGEELPFVGFSYSKALGYLGRSESVVSSLDSPAKVSSMEKKLLIKSKELQDSQDKCHKMEQEMTRLHRRVSEVEAVLSQKEVELKASETQRSLLEQDLATYITECSSLKRSLEQARMEVSQEDDKALQLLHDIREQSRKLQEIKEQEYQAQVEEMRLMMNQLEEDLVSARRRSDLYESELRESRLAAEEFKRKANECQHKLMKVVSHPPRGDSGGTALDDLHKMQGHAGLTSAKDQGKPEVGEYSKLEKINAEQQLKIQELQEKLEKAVKASTEATELLQNIRQAKERAERELEKLHNREDSSEGIKKKLVEAEERRHSLENKVKRLETMERRENRLKDDIQTKSEQIQQMADKILELEEKHREAQVSAQHLEVHLKQKEQHYEEKIKVLDNQIKKDLADKESLENMMQRHEEEAHEKGKILSEQKAMINAMDSKIRSLEQRIVELSEANKLAANSSLFTQRNMKAQEEMISELRQQKFYLETQAGKLEAQNRKLEEQLEKISHQDHSDKSRLLELETRLREVSLEHEEQKLELKRQLTELQLSLQERESQLTALQAARAALESQLRQAKTELEETTAEAEEEIQALTAHRDEIQRKFDALRNSCTVITDLEEQLNQLTEDNAELNNQNFYLSKQLDEASGANDEIVQLRSEVDHLRREITEREMQLTSQKQAQLSAPDLQTMEALKTTCTMLEEQVLDLEALNDELLEKERQWEAWRSVLGDEKSQFECRVRELQRMLDTEKQSRARADQRITESRQVVELAVKEHKAEILALQQALKEQKLKAESLSDKLNDLEKKHAMLEMNARSLQQKLETERELKQRLLEEQAKLQQQMDLQKNHIFRLTQGLQEALDRADLLKTERSDLEYQLENIQVLYSHEKVKMEGTISQQTKLIDFLQAKMDQPAKKKKGLFSRRKEDPALPTQVPLQYNELKLALEKEKARCAELEEALQKTRIELRSAREEAAHRKATDHPHPSTPATARQQIAMSAIVRSPEHQPSAMSLLAPPSSRRKESSTPEEFSRRLKERMHHNIPHRFNVGLNMRATKCAVCLDTVHFGRQASKCLECQVMCHPKCSTCLPATCGLPAEYATHFTEAFCRDKMNSPGLQSKEPGSSLHLEGWMKVPRNNKRGQQGWDRKYIVLEGSKVLIYDNEAREAGQRPVEEFELCLPDGDVSIHGAVGASELANTAKADVPYILKMESHPHTTCWPGRTLYLLAPSFPDKQRWVTALESVVAGGRVSREKAEADAARDHTSSEHQPVWVEKLLGNSLLKLEGDDRLDMNCTLPFSDQVVLVGTEEGLYALNVLKNSLTHIPGIGAVFQIYIIKDLEKLLMIAGEERALCLVDVKKVKQSLAQSHLPAQPDVSPNIFEAVKGCHLFAAGKIENSLCICAAMPSKVVILRYNDNLSKYCIRKEIETSEPCSCIHFTNYSILIGTNKFYEIDMKQYTLDEFLDKNDHSLAPAVFASSSNSFPVSIVQANSAGQREEYLLCFHEFGVFVDSYGRRSRTDDLKWSRLPLAFAYREPYLFVTHFNSLEVIEIQARSSLGSPARAYLEIPNPRYLGPAISSGAIYLASSYQDKLRVICCKGNLVKESGTEQHRVPSTSRSSPNKRGPPTYNEHITKRVASSPAPPEGPSHPREPSTPHRYRDREGRTELRRDKSPGRPLEREKSPGRMLSTRRERSPGRLFEDSSRGRLPAGAVRTPLSQVNKVWDQSSV

Target ORF information:

RefSeq Version	XM_006530131.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus citron (Cit), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006530131.2

ORF Insert Sequence:

ATGTTGAAGT TCAAGTATGG TGTGCGGAAC CCGCCGGAGG CCAGTGCCTC CGAGCCCATT 
GCCAGTCGGG CCTCCAGGCT AAATCTCTTC TTCCAGGGGA AACCGCCCCT CATGACTCAA 
CAGCAGATGT CTGCTCTTTC CCGGGAAGGG ATGCTAGACG CCCTCTTCGC TCTCTTTGAA 
GAGTGCAGCC AACCCGCCCT GATGAAGATG AAGCACGTGA GCAGCTTTGT CCAGAAGTAT 
TCCGACACCA TAGCCGAGTT GCGGGAGCTG CAGCCGTCGG CGAGAGACTT CGAAGTTCGA 
AGCCTTGTGG GCTGTGGTCA CTTCGCTGAA GTGCAGGTGG TTAGAGAGAA GGCGACCGGG 
GACGTCTATG CCATGAAAAT CATGAAGAAG AAGGCTTTGC TGGCCCAGGA ACAGGTTTCA 
TTTTTCGAGG AGGAGAGGAA CATATTATCT CGGAGCACGA GTCCTTGGAT CCCCCAGTTA 
CAGTACGCCT TTCAGGACAA AAATAACCTT TACCTGGTCA TGGAATATCA GCCTGGAGGG 
GATTTGCTGT CGCTTCTGAA CAGATACGAG GACCAATTAG ATGAGAGCAT GATCCAGTTT 
TACCTTGCTG AGCTGATTTT GGCTGTCCAC AGCGTGCACC AGATGGGATA TGTGCATCGA 
GACATCAAGC CCGAGAACAT CCTCATCGAC CGGACGGGAC ACATCAAGCT GGTGGATTTT 
GGATCAGCCG CTAAGATGAA TTCAAATAAG GTGGATGCCA AACTCCCCAT TGGGACCCCG 
GATTACATGG CTCCGGAAGT GTTGACCGTG ATGAACGAGG ACCGAAGGGG CACATACGGC 
TTGGACTGTG ACTGGTGGTC TGTCGGAGTT GTTGCTTATG AGATGGTTTA TGGGAAGACC 
CCATTCACAG AGGGAACCTC CGCCCGGACC TTCAACAACA TCATGAACTT CCAGCGGTTT 
TTGAAGTTCC CAGATGACCC CAAAGTTAGC AGTGAGCTCC TTGATCTGCT TCAGAGTCTG 
CTGTGTGTCC AGAAAGAGAG ACTGAAGTTC GAGGGTCTCT GCTGCCACCC TTTCTTTGCC 
AGAACGGACT GGAACAACAT CCGTAACTCT CCTCCCCCCT TCGTCCCCAC CCTCAAGTCT 
GACGATGACA CCTCCAATTT TGATGAACCA GAGAAGAATT CGTGGGTTTC ATCCTCTGTG 
TGCCAGCTGA GCCCCTCGGG CTTCTCAGGC GAAGAGCTGC CGTTTGTGGG ATTTTCGTAC 
AGCAAGGCAC TGGGGTATCT TGGTAGATCT GAGTCTGTCG TGTCGAGTCT GGACTCCCCT 
GCCAAGGTTA GCTCCATGGA AAAGAAACTT CTCATCAAAA GCAAAGAGCT CCAAGACTCC 
CAGGACAAGT GTCACAAGAT GGAGCAGGAA ATGACCCGGT TACATCGCAG AGTGTCAGAG 
GTGGAGGCTG TGCTTAGTCA GAAGGAGGTG GAGCTGAAGG CCTCTGAGAC TCAGAGATCC 
CTCCTGGAGC AGGACCTTGC TACCTACATC ACAGAATGCA GTAGCTTAAA GCGAAGTTTG 
GAGCAAGCGC GGATGGAGGT GTCCCAGGAG GATGACAAAG CTCTGCAGCT TCTCCACGAC 
ATCCGAGAGC AGAGCCGGAA GCTCCAGGAG ATCAAGGAGC AGGAGTACCA GGCTCAGGTG 
GAGGAGATGA GGCTGATGAT GAATCAGCTG GAAGAAGACC TGGTGTCAGC CCGCAGACGC 
AGCGATCTCT ACGAGTCTGA GCTGAGGGAG TCTCGGCTTG CCGCCGAGGA ATTCAAGCGG 
AAGGCAAACG AATGTCAGCA CAAACTGATG AAGGTAGTCA GCCACCCTCC AAGAGGGGAC 
TCCGGGGGCA CGGCCCTGGA TGATCTTCAC AAGATGCAAG GCCACGCTGG CTTAACGAGT 
GCTAAGGACC AAGGGAAGCC TGAAGTGGGA GAATATTCCA AACTGGAGAA GATCAATGCT 
GAGCAGCAGC TGAAGATCCA GGAGCTCCAG GAGAAGCTGG AGAAGGCTGT AAAAGCCAGC 
ACAGAGGCCA CCGAGCTCCT GCAGAATATC CGCCAGGCAA AGGAGCGAGC AGAGCGGGAG 
CTAGAGAAGC TACACAACCG GGAAGACTCC TCCGAGGGCA TCAAAAAGAA GCTGGTGGAA 
GCCGAGGAAC GCCGCCACTC CCTGGAGAAC AAGGTAAAGA GACTAGAGAC CATGGAGCGT 
AGAGAGAACA GACTGAAGGA TGACATCCAG ACAAAGTCCG AACAGATCCA GCAGATGGCT 
GATAAAATTC TGGAGCTGGA GGAGAAACAT CGGGAGGCTC AGGTCTCAGC TCAACATCTA 
GAAGTACACT TGAAACAGAA AGAACAGCAC TACGAGGAAA AGATCAAGGT ATTGGACAAT 
CAGATAAAGA AGGACCTGGC CGACAAGGAG AGCCTGGAGA ACATGATGCA GAGACACGAG 
GAGGAGGCCC ACGAGAAGGG CAAGATCCTC AGCGAGCAGA AGGCGATGAT CAACGCGATG 
GATTCCAAGA TCCGATCCCT GGAGCAGAGG ATCGTGGAGC TGTCGGAAGC CAACAAGCTT 
GCGGCAAACA GCAGTCTCTT CACCCAGAGG AACATGAAGG CCCAGGAAGA GATGATCTCA 
GAACTCAGGC AGCAGAAATT TTACCTGGAG ACGCAGGCCG GAAAGCTGGA GGCCCAGAAC 
CGAAAGCTGG AAGAGCAACT GGAGAAGATC AGCCACCAAG ATCACAGTGA CAAGAGTCGG 
CTGCTGGAGC TGGAAACAAG GCTGAGGGAG GTCAGCCTGG AGCACGAGGA GCAGAAGCTG 
GAGCTGAAGC GGCAGCTCAC GGAGCTGCAG CTGTCCCTGC AGGAGCGCGA GTCCCAGCTG 
ACGGCCCTGC AGGCCGCCCG GGCAGCGCTG GAGAGCCAGC TCCGCCAGGC GAAGACAGAG 
CTGGAGGAGA CAACCGCGGA AGCGGAGGAG GAGATCCAGG CGCTCACGGC ACATCGAGAT 
GAGATCCAGC GCAAATTCGA TGCCCTTCGC AACAGCTGCA CGGTCATCAC CGACCTGGAG 
GAGCAGCTGA ACCAGCTCAC CGAGGACAAC GCCGAGCTCA ACAACCAAAA CTTCTACCTG 
TCCAAACAAC TCGATGAGGC TTCCGGGGCC AATGACGAGA TTGTGCAGCT GCGGAGTGAG 
GTGGACCATC TCCGCCGTGA GATCACGGAG CGGGAGATGC AGCTCACCAG CCAGAAGCAA 
GCACAACTCA GTGCTCCTGA TTTGCAGACG ATGGAGGCTC TGAAGACGAC ATGCACCATG 
CTGGAGGAGC AGGTCCTGGA CCTGGAGGCC CTGAACGACG AGCTGCTGGA GAAGGAGCGC 
CAGTGGGAGG CCTGGCGGAG CGTCCTCGGC GACGAGAAGT CCCAGTTCGA GTGTCGAGTT 
CGAGAGCTAC AGAGGATGCT GGACACCGAG AAGCAGAGCA GGGCTAGGGC CGATCAGCGG 
ATCACCGAGT CGCGCCAGGT GGTGGAGTTG GCGGTGAAGG AACACAAGGC CGAGATTCTT 
GCTCTGCAGC AGGCTCTCAA GGAGCAGAAG CTCAAAGCCG AGAGCCTGTC GGACAAGCTC 
AACGACCTGG AGAAGAAGCA CGCCATGCTG GAGATGAACG CCCGGAGCTT ACAGCAGAAA 
CTAGAGACAG AGCGGGAGCT CAAACAGAGG CTTCTGGAGG AGCAAGCCAA ATTGCAGCAG 
CAGATGGACC TGCAGAAGAA CCACATCTTC AGACTGACGC AAGGGCTGCA GGAGGCGCTG 
GACCGGGCGG ATCTGCTGAA GACAGAAAGG AGCGACCTGG AGTACCAGCT GGAAAACATT 
CAGGTTCTCT ACTCTCACGA GAAAGTGAAA ATGGAAGGCA CAATCTCTCA GCAAACCAAA 
CTCATTGATT TCCTGCAAGC CAAAATGGAC CAGCCTGCTA AAAAGAAAAA GGGTTTATTT 
AGTCGACGGA AAGAGGACCC TGCTTTGCCC ACACAGGTTC CTCTGCAGTA CAATGAGCTG 
AAGCTAGCCC TGGAAAAGGA GAAAGCCCGA TGCGCGGAGC TGGAGGAGGC CCTTCAGAAG 
ACCCGCATCG AACTCCGCTC TGCGCGGGAG GAAGCTGCCC ACCGCAAAGC CACGGACCAC 
CCGCACCCGT CTACGCCAGC CACTGCGAGG CAGCAGATCG CCATGTCTGC CATTGTGCGG 
TCGCCCGAGC ACCAGCCCAG TGCCATGAGC CTGCTTGCCC CACCATCCAG CCGCAGAAAG 
GAGTCGTCAA CTCCAGAGGA ATTCAGCCGG CGTCTGAAAG AGCGCATGCA CCACAACATC 
CCTCACCGGT TTAATGTGGG CCTGAACATG AGAGCCACCA AGTGCGCCGT GTGTCTGGAT 
ACTGTGCACT TTGGACGCCA GGCATCCAAA TGCCTCGAAT GTCAAGTGAT GTGTCACCCC 
AAATGCTCCA CCTGCTTGCC CGCTACCTGT GGCCTGCCAG CTGAATATGC CACACACTTC 
ACTGAGGCCT TCTGCCGGGA CAAAATGAAC TCCCCGGGGC TCCAGAGCAA GGAGCCTGGC 
AGCAGCTTGC ACCTGGAAGG GTGGATGAAA GTGCCCAGAA ATAACAAACG GGGACAGCAA 
GGCTGGGACA GGAAGTACAT TGTTCTGGAG GGGTCAAAAG TCCTCATCTA TGACAATGAA 
GCCAGAGAAG CTGGACAGAG GCCGGTGGAA GAATTTGAGC TGTGCCTTCC TGACGGGGAC 
GTATCTATTC ATGGTGCCGT TGGTGCTTCA GAACTTGCAA ATACCGCCAA AGCAGATGTC 
CCATACATCC TGAAGATGGA GTCTCACCCA CACACCACCT GCTGGCCTGG GAGAACCCTC 
TACTTGCTAG CACCCAGCTT CCCCGACAAG CAGCGCTGGG TCACCGCCTT AGAATCTGTC 
GTCGCAGGTG GGAGAGTTTC TAGGGAAAAG GCCGAAGCCG ATGCTGCACG TGACCATACT 
TCGAGTGAGC ATCAGCCAGT GTGGGTTGAG AAATTACTTG GAAACTCTCT GCTGAAACTG 
GAGGGCGATG ACCGGCTTGA CATGAACTGC ACCCTGCCCT TCAGTGACCA GGTAGTGCTG 
GTGGGCACGG AGGAAGGCCT CTACGCCCTG AATGTCTTGA AAAACTCCTT AACCCACATC 
CCAGGGATTG GCGCAGTCTT CCAAATTTAC ATCATCAAGG ACCTGGAGAA GCTGCTCATG 
ATAGCAGGGG AAGAGCGGGC TCTGTGCCTG GTGGACGTGA AGAAGGTGAA GCAGTCCCTG 
GCGCAGTCAC ACCTGCCTGC CCAGCCCGAC GTCTCCCCCA ACATATTCGA AGCCGTCAAA 
GGCTGCCACT TGTTCGCTGC TGGCAAGATC GAGAACAGCC TGTGCATCTG CGCCGCTATG 
CCAAGCAAAG TCGTCATCCT CCGCTACAAT GACAACCTCA GCAAGTACTG CATCCGCAAG 
GAGATCGAGA CCTCAGAGCC CTGCAGCTGT ATCCACTTCA CCAACTACAG CATCCTCATT 
GGGACCAACA AATTCTATGA GATCGACATG AAGCAGTACA CGCTTGACGA GTTCCTGGAC 
AAGAACGACC ATTCCTTGGC ACCTGCTGTG TTCGCCTCCT CGTCCAACAG CTTCCCTGTC 
TCCATTGTGC AGGCGAACAG CGCCGGGCAG CGAGAAGAAT ACCTGCTGTG CTTCCACGAA 
TTTGGGGTGT TCGTGGATTC TTACGGAAGA CGTAGCCGCA CAGATGATCT CAAGTGGAGT 
CGCTTACCTC TGGCCTTCGC CTACAGAGAA CCTTATCTGT TTGTGACTCA CTTCAACTCC 
CTGGAAGTCA TTGAGATCCA GGCCAGATCC TCACTGGGGA GCCCTGCCCG AGCATATCTG 
GAAATTCCAA ACCCTCGCTA CCTGGGCCCC GCGATTTCCT CCGGAGCGAT TTACCTGGCC 
TCCTCATACC AGGACAAGTT AAGGGTCATA TGCTGCAAAG GAAACCTCGT GAAGGAGTCA 
GGCACTGAGC AGCACCGGGT GCCCTCCACC TCCCGCAGCA GCCCCAACAA GCGAGGCCCA 
CCAACATACA ACGAGCACAT CACCAAACGC GTGGCCTCCA GCCCGGCGCC ACCGGAAGGC 
CCCAGCCACC CCCGAGAGCC AAGCACACCG CACCGCTACC GAGACAGAGA GGGTCGGACA 
GAGCTGCGCA GGGACAAGTC TCCAGGCCGC CCTCTGGAGC GGGAGAAGTC CCCAGGCCGA 
ATGCTCAGCA CTAGGAGAGA GCGGTCCCCA GGGAGACTGT TTGAAGACAG CAGCAGGGGC 
CGGCTGCCTG CAGGAGCAGT GAGGACCCCA CTGTCCCAGG TTAACAAGGT CTGGGACCAG 
TCTTCAGTAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cit ( XM_006530133.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006530196.1 Mus musculus citron (Cit), transcript variant X4, mRNA.

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CloneID	OMu35774
Clone ID Related Accession (Same CDS sequence)	XM_006530130.3 , XM_006530132.3 , XM_006530133.3 , XM_006530130.4 , XM_006530131.2 , XM_006530131.1 , XM_006530133.4 , XM_006530132.4
Accession Version	XM_006530133.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6372bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	citron Rho-interacting kinase isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000071.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006530133.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTGAAGT TCAAGTATGG TGTGCGGAAC CCGCCGGAGG CCAGTGCCTC CGAGCCCATT 
GCCAGTCGGG CCTCCAGGCT AAATCTCTTC TTCCAGGGGA AACCGCCCCT CATGACTCAA 
CAGCAGATGT CTGCTCTTTC CCGGGAAGGG ATGCTAGACG CCCTCTTCGC TCTCTTTGAA 
GAGTGCAGCC AACCCGCCCT GATGAAGATG AAGCACGTGA GCAGCTTTGT CCAGAAGTAT 
TCCGACACCA TAGCCGAGTT GCGGGAGCTG CAGCCGTCGG CGAGAGACTT CGAAGTTCGA 
AGCCTTGTGG GCTGTGGTCA CTTCGCTGAA GTGCAGGTGG TTAGAGAGAA GGCGACCGGG 
GACGTCTATG CCATGAAAAT CATGAAGAAG AAGGCTTTGC TGGCCCAGGA ACAGGTTTCA 
TTTTTCGAGG AGGAGAGGAA CATATTATCT CGGAGCACGA GTCCTTGGAT CCCCCAGTTA 
CAGTACGCCT TTCAGGACAA AAATAACCTT TACCTGGTCA TGGAATATCA GCCTGGAGGG 
GATTTGCTGT CGCTTCTGAA CAGATACGAG GACCAATTAG ATGAGAGCAT GATCCAGTTT 
TACCTTGCTG AGCTGATTTT GGCTGTCCAC AGCGTGCACC AGATGGGATA TGTGCATCGA 
GACATCAAGC CCGAGAACAT CCTCATCGAC CGGACGGGAC ACATCAAGCT GGTGGATTTT 
GGATCAGCCG CTAAGATGAA TTCAAATAAG GTGGATGCCA AACTCCCCAT TGGGACCCCG 
GATTACATGG CTCCGGAAGT GTTGACCGTG ATGAACGAGG ACCGAAGGGG CACATACGGC 
TTGGACTGTG ACTGGTGGTC TGTCGGAGTT GTTGCTTATG AGATGGTTTA TGGGAAGACC 
CCATTCACAG AGGGAACCTC CGCCCGGACC TTCAACAACA TCATGAACTT CCAGCGGTTT 
TTGAAGTTCC CAGATGACCC CAAAGTTAGC AGTGAGCTCC TTGATCTGCT TCAGAGTCTG 
CTGTGTGTCC AGAAAGAGAG ACTGAAGTTC GAGGGTCTCT GCTGCCACCC TTTCTTTGCC 
AGAACGGACT GGAACAACAT CCGTAACTCT CCTCCCCCCT TCGTCCCCAC CCTCAAGTCT 
GACGATGACA CCTCCAATTT TGATGAACCA GAGAAGAATT CGTGGGTTTC ATCCTCTGTG 
TGCCAGCTGA GCCCCTCGGG CTTCTCAGGC GAAGAGCTGC CGTTTGTGGG ATTTTCGTAC 
AGCAAGGCAC TGGGGTATCT TGGTAGATCT GAGTCTGTCG TGTCGAGTCT GGACTCCCCT 
GCCAAGGTTA GCTCCATGGA AAAGAAACTT CTCATCAAAA GCAAAGAGCT CCAAGACTCC 
CAGGACAAGT GTCACAAGAT GGAGCAGGAA ATGACCCGGT TACATCGCAG AGTGTCAGAG 
GTGGAGGCTG TGCTTAGTCA GAAGGAGGTG GAGCTGAAGG CCTCTGAGAC TCAGAGATCC 
CTCCTGGAGC AGGACCTTGC TACCTACATC ACAGAATGCA GTAGCTTAAA GCGAAGTTTG 
GAGCAAGCGC GGATGGAGGT GTCCCAGGAG GATGACAAAG CTCTGCAGCT TCTCCACGAC 
ATCCGAGAGC AGAGCCGGAA GCTCCAGGAG ATCAAGGAGC AGGAGTACCA GGCTCAGGTG 
GAGGAGATGA GGCTGATGAT GAATCAGCTG GAAGAAGACC TGGTGTCAGC CCGCAGACGC 
AGCGATCTCT ACGAGTCTGA GCTGAGGGAG TCTCGGCTTG CCGCCGAGGA ATTCAAGCGG 
AAGGCAAACG AATGTCAGCA CAAACTGATG AAGGTAGTCA GCCACCCTCC AAGAGGGGAC 
TCCGGGGGCA CGGCCCTGGA TGATCTTCAC AAGATGCAAG GCCACGCTGG CTTAACGAGT 
GCTAAGGACC AAGGGAAGCC TGAAGTGGGA GAATATTCCA AACTGGAGAA GATCAATGCT 
GAGCAGCAGC TGAAGATCCA GGAGCTCCAG GAGAAGCTGG AGAAGGCTGT AAAAGCCAGC 
ACAGAGGCCA CCGAGCTCCT GCAGAATATC CGCCAGGCAA AGGAGCGAGC AGAGCGGGAG 
CTAGAGAAGC TACACAACCG GGAAGACTCC TCCGAGGGCA TCAAAAAGAA GCTGGTGGAA 
GCCGAGGAAC GCCGCCACTC CCTGGAGAAC AAGGTAAAGA GACTAGAGAC CATGGAGCGT 
AGAGAGAACA GACTGAAGGA TGACATCCAG ACAAAGTCCG AACAGATCCA GCAGATGGCT 
GATAAAATTC TGGAGCTGGA GGAGAAACAT CGGGAGGCTC AGGTCTCAGC TCAACATCTA 
GAAGTACACT TGAAACAGAA AGAACAGCAC TACGAGGAAA AGATCAAGGT ATTGGACAAT 
CAGATAAAGA AGGACCTGGC CGACAAGGAG AGCCTGGAGA ACATGATGCA GAGACACGAG 
GAGGAGGCCC ACGAGAAGGG CAAGATCCTC AGCGAGCAGA AGGCGATGAT CAACGCGATG 
GATTCCAAGA TCCGATCCCT GGAGCAGAGG ATCGTGGAGC TGTCGGAAGC CAACAAGCTT 
GCGGCAAACA GCAGTCTCTT CACCCAGAGG AACATGAAGG CCCAGGAAGA GATGATCTCA 
GAACTCAGGC AGCAGAAATT TTACCTGGAG ACGCAGGCCG GAAAGCTGGA GGCCCAGAAC 
CGAAAGCTGG AAGAGCAACT GGAGAAGATC AGCCACCAAG ATCACAGTGA CAAGAGTCGG 
CTGCTGGAGC TGGAAACAAG GCTGAGGGAG GTCAGCCTGG AGCACGAGGA GCAGAAGCTG 
GAGCTGAAGC GGCAGCTCAC GGAGCTGCAG CTGTCCCTGC AGGAGCGCGA GTCCCAGCTG 
ACGGCCCTGC AGGCCGCCCG GGCAGCGCTG GAGAGCCAGC TCCGCCAGGC GAAGACAGAG 
CTGGAGGAGA CAACCGCGGA AGCGGAGGAG GAGATCCAGG CGCTCACGGC ACATCGAGAT 
GAGATCCAGC GCAAATTCGA TGCCCTTCGC AACAGCTGCA CGGTCATCAC CGACCTGGAG 
GAGCAGCTGA ACCAGCTCAC CGAGGACAAC GCCGAGCTCA ACAACCAAAA CTTCTACCTG 
TCCAAACAAC TCGATGAGGC TTCCGGGGCC AATGACGAGA TTGTGCAGCT GCGGAGTGAG 
GTGGACCATC TCCGCCGTGA GATCACGGAG CGGGAGATGC AGCTCACCAG CCAGAAGCAA 
GCACAACTCA GTGCTCCTGA TTTGCAGACG ATGGAGGCTC TGAAGACGAC ATGCACCATG 
CTGGAGGAGC AGGTCCTGGA CCTGGAGGCC CTGAACGACG AGCTGCTGGA GAAGGAGCGC 
CAGTGGGAGG CCTGGCGGAG CGTCCTCGGC GACGAGAAGT CCCAGTTCGA GTGTCGAGTT 
CGAGAGCTAC AGAGGATGCT GGACACCGAG AAGCAGAGCA GGGCTAGGGC CGATCAGCGG 
ATCACCGAGT CGCGCCAGGT GGTGGAGTTG GCGGTGAAGG AACACAAGGC CGAGATTCTT 
GCTCTGCAGC AGGCTCTCAA GGAGCAGAAG CTCAAAGCCG AGAGCCTGTC GGACAAGCTC 
AACGACCTGG AGAAGAAGCA CGCCATGCTG GAGATGAACG CCCGGAGCTT ACAGCAGAAA 
CTAGAGACAG AGCGGGAGCT CAAACAGAGG CTTCTGGAGG AGCAAGCCAA ATTGCAGCAG 
CAGATGGACC TGCAGAAGAA CCACATCTTC AGACTGACGC AAGGGCTGCA GGAGGCGCTG 
GACCGGGCGG ATCTGCTGAA GACAGAAAGG AGCGACCTGG AGTACCAGCT GGAAAACATT 
CAGGTTCTCT ACTCTCACGA GAAAGTGAAA ATGGAAGGCA CAATCTCTCA GCAAACCAAA 
CTCATTGATT TCCTGCAAGC CAAAATGGAC CAGCCTGCTA AAAAGAAAAA GGGTTTATTT 
AGTCGACGGA AAGAGGACCC TGCTTTGCCC ACACAGGTTC CTCTGCAGTA CAATGAGCTG 
AAGCTAGCCC TGGAAAAGGA GAAAGCCCGA TGCGCGGAGC TGGAGGAGGC CCTTCAGAAG 
ACCCGCATCG AACTCCGCTC TGCGCGGGAG GAAGCTGCCC ACCGCAAAGC CACGGACCAC 
CCGCACCCGT CTACGCCAGC CACTGCGAGG CAGCAGATCG CCATGTCTGC CATTGTGCGG 
TCGCCCGAGC ACCAGCCCAG TGCCATGAGC CTGCTTGCCC CACCATCCAG CCGCAGAAAG 
GAGTCGTCAA CTCCAGAGGA ATTCAGCCGG CGTCTGAAAG AGCGCATGCA CCACAACATC 
CCTCACCGGT TTAATGTGGG CCTGAACATG AGAGCCACCA AGTGCGCCGT GTGTCTGGAT 
ACTGTGCACT TTGGACGCCA GGCATCCAAA TGCCTCGAAT GTCAAGTGAT GTGTCACCCC 
AAATGCTCCA CCTGCTTGCC CGCTACCTGT GGCCTGCCAG CTGAATATGC CACACACTTC 
ACTGAGGCCT TCTGCCGGGA CAAAATGAAC TCCCCGGGGC TCCAGAGCAA GGAGCCTGGC 
AGCAGCTTGC ACCTGGAAGG GTGGATGAAA GTGCCCAGAA ATAACAAACG GGGACAGCAA 
GGCTGGGACA GGAAGTACAT TGTTCTGGAG GGGTCAAAAG TCCTCATCTA TGACAATGAA 
GCCAGAGAAG CTGGACAGAG GCCGGTGGAA GAATTTGAGC TGTGCCTTCC TGACGGGGAC 
GTATCTATTC ATGGTGCCGT TGGTGCTTCA GAACTTGCAA ATACCGCCAA AGCAGATGTC 
CCATACATCC TGAAGATGGA GTCTCACCCA CACACCACCT GCTGGCCTGG GAGAACCCTC 
TACTTGCTAG CACCCAGCTT CCCCGACAAG CAGCGCTGGG TCACCGCCTT AGAATCTGTC 
GTCGCAGGTG GGAGAGTTTC TAGGGAAAAG GCCGAAGCCG ATGCTGCACG TGACCATACT 
TCGAGTGAGC ATCAGCCAGT GTGGGTTGAG AAATTACTTG GAAACTCTCT GCTGAAACTG 
GAGGGCGATG ACCGGCTTGA CATGAACTGC ACCCTGCCCT TCAGTGACCA GGTAGTGCTG 
GTGGGCACGG AGGAAGGCCT CTACGCCCTG AATGTCTTGA AAAACTCCTT AACCCACATC 
CCAGGGATTG GCGCAGTCTT CCAAATTTAC ATCATCAAGG ACCTGGAGAA GCTGCTCATG 
ATAGCAGGGG AAGAGCGGGC TCTGTGCCTG GTGGACGTGA AGAAGGTGAA GCAGTCCCTG 
GCGCAGTCAC ACCTGCCTGC CCAGCCCGAC GTCTCCCCCA ACATATTCGA AGCCGTCAAA 
GGCTGCCACT TGTTCGCTGC TGGCAAGATC GAGAACAGCC TGTGCATCTG CGCCGCTATG 
CCAAGCAAAG TCGTCATCCT CCGCTACAAT GACAACCTCA GCAAGTACTG CATCCGCAAG 
GAGATCGAGA CCTCAGAGCC CTGCAGCTGT ATCCACTTCA CCAACTACAG CATCCTCATT 
GGGACCAACA AATTCTATGA GATCGACATG AAGCAGTACA CGCTTGACGA GTTCCTGGAC 
AAGAACGACC ATTCCTTGGC ACCTGCTGTG TTCGCCTCCT CGTCCAACAG CTTCCCTGTC 
TCCATTGTGC AGGCGAACAG CGCCGGGCAG CGAGAAGAAT ACCTGCTGTG CTTCCACGAA 
TTTGGGGTGT TCGTGGATTC TTACGGAAGA CGTAGCCGCA CAGATGATCT CAAGTGGAGT 
CGCTTACCTC TGGCCTTCGC CTACAGAGAA CCTTATCTGT TTGTGACTCA CTTCAACTCC 
CTGGAAGTCA TTGAGATCCA GGCCAGATCC TCACTGGGGA GCCCTGCCCG AGCATATCTG 
GAAATTCCAA ACCCTCGCTA CCTGGGCCCC GCGATTTCCT CCGGAGCGAT TTACCTGGCC 
TCCTCATACC AGGACAAGTT AAGGGTCATA TGCTGCAAAG GAAACCTCGT GAAGGAGTCA 
GGCACTGAGC AGCACCGGGT GCCCTCCACC TCCCGCAGCA GCCCCAACAA GCGAGGCCCA 
CCAACATACA ACGAGCACAT CACCAAACGC GTGGCCTCCA GCCCGGCGCC ACCGGAAGGC 
CCCAGCCACC CCCGAGAGCC AAGCACACCG CACCGCTACC GAGACAGAGA GGGTCGGACA 
GAGCTGCGCA GGGACAAGTC TCCAGGCCGC CCTCTGGAGC GGGAGAAGTC CCCAGGCCGA 
ATGCTCAGCA CTAGGAGAGA GCGGTCCCCA GGGAGACTGT TTGAAGACAG CAGCAGGGGC 
CGGCTGCCTG CAGGAGCAGT GAGGACCCCA CTGTCCCAGG TTAACAAGGT CTGGGACCAG 
TCTTCAGTAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006530196.1
CDS	115..6486
Translation	MLKFKYGVRNPPEASASEPIASRASRLNLFFQGKPPLMTQQQMSALSREGMLDALFALFEECSQPALMKMKHVSSFVQKYSDTIAELRELQPSARDFEVRSLVGCGHFAEVQVVREKATGDVYAMKIMKKKALLAQEQVSFFEEERNILSRSTSPWIPQLQYAFQDKNNLYLVMEYQPGGDLLSLLNRYEDQLDESMIQFYLAELILAVHSVHQMGYVHRDIKPENILIDRTGHIKLVDFGSAAKMNSNKVDAKLPIGTPDYMAPEVLTVMNEDRRGTYGLDCDWWSVGVVAYEMVYGKTPFTEGTSARTFNNIMNFQRFLKFPDDPKVSSELLDLLQSLLCVQKERLKFEGLCCHPFFARTDWNNIRNSPPPFVPTLKSDDDTSNFDEPEKNSWVSSSVCQLSPSGFSGEELPFVGFSYSKALGYLGRSESVVSSLDSPAKVSSMEKKLLIKSKELQDSQDKCHKMEQEMTRLHRRVSEVEAVLSQKEVELKASETQRSLLEQDLATYITECSSLKRSLEQARMEVSQEDDKALQLLHDIREQSRKLQEIKEQEYQAQVEEMRLMMNQLEEDLVSARRRSDLYESELRESRLAAEEFKRKANECQHKLMKVVSHPPRGDSGGTALDDLHKMQGHAGLTSAKDQGKPEVGEYSKLEKINAEQQLKIQELQEKLEKAVKASTEATELLQNIRQAKERAERELEKLHNREDSSEGIKKKLVEAEERRHSLENKVKRLETMERRENRLKDDIQTKSEQIQQMADKILELEEKHREAQVSAQHLEVHLKQKEQHYEEKIKVLDNQIKKDLADKESLENMMQRHEEEAHEKGKILSEQKAMINAMDSKIRSLEQRIVELSEANKLAANSSLFTQRNMKAQEEMISELRQQKFYLETQAGKLEAQNRKLEEQLEKISHQDHSDKSRLLELETRLREVSLEHEEQKLELKRQLTELQLSLQERESQLTALQAARAALESQLRQAKTELEETTAEAEEEIQALTAHRDEIQRKFDALRNSCTVITDLEEQLNQLTEDNAELNNQNFYLSKQLDEASGANDEIVQLRSEVDHLRREITEREMQLTSQKQAQLSAPDLQTMEALKTTCTMLEEQVLDLEALNDELLEKERQWEAWRSVLGDEKSQFECRVRELQRMLDTEKQSRARADQRITESRQVVELAVKEHKAEILALQQALKEQKLKAESLSDKLNDLEKKHAMLEMNARSLQQKLETERELKQRLLEEQAKLQQQMDLQKNHIFRLTQGLQEALDRADLLKTERSDLEYQLENIQVLYSHEKVKMEGTISQQTKLIDFLQAKMDQPAKKKKGLFSRRKEDPALPTQVPLQYNELKLALEKEKARCAELEEALQKTRIELRSAREEAAHRKATDHPHPSTPATARQQIAMSAIVRSPEHQPSAMSLLAPPSSRRKESSTPEEFSRRLKERMHHNIPHRFNVGLNMRATKCAVCLDTVHFGRQASKCLECQVMCHPKCSTCLPATCGLPAEYATHFTEAFCRDKMNSPGLQSKEPGSSLHLEGWMKVPRNNKRGQQGWDRKYIVLEGSKVLIYDNEAREAGQRPVEEFELCLPDGDVSIHGAVGASELANTAKADVPYILKMESHPHTTCWPGRTLYLLAPSFPDKQRWVTALESVVAGGRVSREKAEADAARDHTSSEHQPVWVEKLLGNSLLKLEGDDRLDMNCTLPFSDQVVLVGTEEGLYALNVLKNSLTHIPGIGAVFQIYIIKDLEKLLMIAGEERALCLVDVKKVKQSLAQSHLPAQPDVSPNIFEAVKGCHLFAAGKIENSLCICAAMPSKVVILRYNDNLSKYCIRKEIETSEPCSCIHFTNYSILIGTNKFYEIDMKQYTLDEFLDKNDHSLAPAVFASSSNSFPVSIVQANSAGQREEYLLCFHEFGVFVDSYGRRSRTDDLKWSRLPLAFAYREPYLFVTHFNSLEVIEIQARSSLGSPARAYLEIPNPRYLGPAISSGAIYLASSYQDKLRVICCKGNLVKESGTEQHRVPSTSRSSPNKRGPPTYNEHITKRVASSPAPPEGPSHPREPSTPHRYRDREGRTELRRDKSPGRPLEREKSPGRMLSTRRERSPGRLFEDSSRGRLPAGAVRTPLSQVNKVWDQSSV

Target ORF information:

RefSeq Version	XM_006530133.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus citron (Cit), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006530133.4

ORF Insert Sequence:

ATGTTGAAGT TCAAGTATGG TGTGCGGAAC CCGCCGGAGG CCAGTGCCTC CGAGCCCATT 
GCCAGTCGGG CCTCCAGGCT AAATCTCTTC TTCCAGGGGA AACCGCCCCT CATGACTCAA 
CAGCAGATGT CTGCTCTTTC CCGGGAAGGG ATGCTAGACG CCCTCTTCGC TCTCTTTGAA 
GAGTGCAGCC AACCCGCCCT GATGAAGATG AAGCACGTGA GCAGCTTTGT CCAGAAGTAT 
TCCGACACCA TAGCCGAGTT GCGGGAGCTG CAGCCGTCGG CGAGAGACTT CGAAGTTCGA 
AGCCTTGTGG GCTGTGGTCA CTTCGCTGAA GTGCAGGTGG TTAGAGAGAA GGCGACCGGG 
GACGTCTATG CCATGAAAAT CATGAAGAAG AAGGCTTTGC TGGCCCAGGA ACAGGTTTCA 
TTTTTCGAGG AGGAGAGGAA CATATTATCT CGGAGCACGA GTCCTTGGAT CCCCCAGTTA 
CAGTACGCCT TTCAGGACAA AAATAACCTT TACCTGGTCA TGGAATATCA GCCTGGAGGG 
GATTTGCTGT CGCTTCTGAA CAGATACGAG GACCAATTAG ATGAGAGCAT GATCCAGTTT 
TACCTTGCTG AGCTGATTTT GGCTGTCCAC AGCGTGCACC AGATGGGATA TGTGCATCGA 
GACATCAAGC CCGAGAACAT CCTCATCGAC CGGACGGGAC ACATCAAGCT GGTGGATTTT 
GGATCAGCCG CTAAGATGAA TTCAAATAAG GTGGATGCCA AACTCCCCAT TGGGACCCCG 
GATTACATGG CTCCGGAAGT GTTGACCGTG ATGAACGAGG ACCGAAGGGG CACATACGGC 
TTGGACTGTG ACTGGTGGTC TGTCGGAGTT GTTGCTTATG AGATGGTTTA TGGGAAGACC 
CCATTCACAG AGGGAACCTC CGCCCGGACC TTCAACAACA TCATGAACTT CCAGCGGTTT 
TTGAAGTTCC CAGATGACCC CAAAGTTAGC AGTGAGCTCC TTGATCTGCT TCAGAGTCTG 
CTGTGTGTCC AGAAAGAGAG ACTGAAGTTC GAGGGTCTCT GCTGCCACCC TTTCTTTGCC 
AGAACGGACT GGAACAACAT CCGTAACTCT CCTCCCCCCT TCGTCCCCAC CCTCAAGTCT 
GACGATGACA CCTCCAATTT TGATGAACCA GAGAAGAATT CGTGGGTTTC ATCCTCTGTG 
TGCCAGCTGA GCCCCTCGGG CTTCTCAGGC GAAGAGCTGC CGTTTGTGGG ATTTTCGTAC 
AGCAAGGCAC TGGGGTATCT TGGTAGATCT GAGTCTGTCG TGTCGAGTCT GGACTCCCCT 
GCCAAGGTTA GCTCCATGGA AAAGAAACTT CTCATCAAAA GCAAAGAGCT CCAAGACTCC 
CAGGACAAGT GTCACAAGAT GGAGCAGGAA ATGACCCGGT TACATCGCAG AGTGTCAGAG 
GTGGAGGCTG TGCTTAGTCA GAAGGAGGTG GAGCTGAAGG CCTCTGAGAC TCAGAGATCC 
CTCCTGGAGC AGGACCTTGC TACCTACATC ACAGAATGCA GTAGCTTAAA GCGAAGTTTG 
GAGCAAGCGC GGATGGAGGT GTCCCAGGAG GATGACAAAG CTCTGCAGCT TCTCCACGAC 
ATCCGAGAGC AGAGCCGGAA GCTCCAGGAG ATCAAGGAGC AGGAGTACCA GGCTCAGGTG 
GAGGAGATGA GGCTGATGAT GAATCAGCTG GAAGAAGACC TGGTGTCAGC CCGCAGACGC 
AGCGATCTCT ACGAGTCTGA GCTGAGGGAG TCTCGGCTTG CCGCCGAGGA ATTCAAGCGG 
AAGGCAAACG AATGTCAGCA CAAACTGATG AAGGTAGTCA GCCACCCTCC AAGAGGGGAC 
TCCGGGGGCA CGGCCCTGGA TGATCTTCAC AAGATGCAAG GCCACGCTGG CTTAACGAGT 
GCTAAGGACC AAGGGAAGCC TGAAGTGGGA GAATATTCCA AACTGGAGAA GATCAATGCT 
GAGCAGCAGC TGAAGATCCA GGAGCTCCAG GAGAAGCTGG AGAAGGCTGT AAAAGCCAGC 
ACAGAGGCCA CCGAGCTCCT GCAGAATATC CGCCAGGCAA AGGAGCGAGC AGAGCGGGAG 
CTAGAGAAGC TACACAACCG GGAAGACTCC TCCGAGGGCA TCAAAAAGAA GCTGGTGGAA 
GCCGAGGAAC GCCGCCACTC CCTGGAGAAC AAGGTAAAGA GACTAGAGAC CATGGAGCGT 
AGAGAGAACA GACTGAAGGA TGACATCCAG ACAAAGTCCG AACAGATCCA GCAGATGGCT 
GATAAAATTC TGGAGCTGGA GGAGAAACAT CGGGAGGCTC AGGTCTCAGC TCAACATCTA 
GAAGTACACT TGAAACAGAA AGAACAGCAC TACGAGGAAA AGATCAAGGT ATTGGACAAT 
CAGATAAAGA AGGACCTGGC CGACAAGGAG AGCCTGGAGA ACATGATGCA GAGACACGAG 
GAGGAGGCCC ACGAGAAGGG CAAGATCCTC AGCGAGCAGA AGGCGATGAT CAACGCGATG 
GATTCCAAGA TCCGATCCCT GGAGCAGAGG ATCGTGGAGC TGTCGGAAGC CAACAAGCTT 
GCGGCAAACA GCAGTCTCTT CACCCAGAGG AACATGAAGG CCCAGGAAGA GATGATCTCA 
GAACTCAGGC AGCAGAAATT TTACCTGGAG ACGCAGGCCG GAAAGCTGGA GGCCCAGAAC 
CGAAAGCTGG AAGAGCAACT GGAGAAGATC AGCCACCAAG ATCACAGTGA CAAGAGTCGG 
CTGCTGGAGC TGGAAACAAG GCTGAGGGAG GTCAGCCTGG AGCACGAGGA GCAGAAGCTG 
GAGCTGAAGC GGCAGCTCAC GGAGCTGCAG CTGTCCCTGC AGGAGCGCGA GTCCCAGCTG 
ACGGCCCTGC AGGCCGCCCG GGCAGCGCTG GAGAGCCAGC TCCGCCAGGC GAAGACAGAG 
CTGGAGGAGA CAACCGCGGA AGCGGAGGAG GAGATCCAGG CGCTCACGGC ACATCGAGAT 
GAGATCCAGC GCAAATTCGA TGCCCTTCGC AACAGCTGCA CGGTCATCAC CGACCTGGAG 
GAGCAGCTGA ACCAGCTCAC CGAGGACAAC GCCGAGCTCA ACAACCAAAA CTTCTACCTG 
TCCAAACAAC TCGATGAGGC TTCCGGGGCC AATGACGAGA TTGTGCAGCT GCGGAGTGAG 
GTGGACCATC TCCGCCGTGA GATCACGGAG CGGGAGATGC AGCTCACCAG CCAGAAGCAA 
GCACAACTCA GTGCTCCTGA TTTGCAGACG ATGGAGGCTC TGAAGACGAC ATGCACCATG 
CTGGAGGAGC AGGTCCTGGA CCTGGAGGCC CTGAACGACG AGCTGCTGGA GAAGGAGCGC 
CAGTGGGAGG CCTGGCGGAG CGTCCTCGGC GACGAGAAGT CCCAGTTCGA GTGTCGAGTT 
CGAGAGCTAC AGAGGATGCT GGACACCGAG AAGCAGAGCA GGGCTAGGGC CGATCAGCGG 
ATCACCGAGT CGCGCCAGGT GGTGGAGTTG GCGGTGAAGG AACACAAGGC CGAGATTCTT 
GCTCTGCAGC AGGCTCTCAA GGAGCAGAAG CTCAAAGCCG AGAGCCTGTC GGACAAGCTC 
AACGACCTGG AGAAGAAGCA CGCCATGCTG GAGATGAACG CCCGGAGCTT ACAGCAGAAA 
CTAGAGACAG AGCGGGAGCT CAAACAGAGG CTTCTGGAGG AGCAAGCCAA ATTGCAGCAG 
CAGATGGACC TGCAGAAGAA CCACATCTTC AGACTGACGC AAGGGCTGCA GGAGGCGCTG 
GACCGGGCGG ATCTGCTGAA GACAGAAAGG AGCGACCTGG AGTACCAGCT GGAAAACATT 
CAGGTTCTCT ACTCTCACGA GAAAGTGAAA ATGGAAGGCA CAATCTCTCA GCAAACCAAA 
CTCATTGATT TCCTGCAAGC CAAAATGGAC CAGCCTGCTA AAAAGAAAAA GGGTTTATTT 
AGTCGACGGA AAGAGGACCC TGCTTTGCCC ACACAGGTTC CTCTGCAGTA CAATGAGCTG 
AAGCTAGCCC TGGAAAAGGA GAAAGCCCGA TGCGCGGAGC TGGAGGAGGC CCTTCAGAAG 
ACCCGCATCG AACTCCGCTC TGCGCGGGAG GAAGCTGCCC ACCGCAAAGC CACGGACCAC 
CCGCACCCGT CTACGCCAGC CACTGCGAGG CAGCAGATCG CCATGTCTGC CATTGTGCGG 
TCGCCCGAGC ACCAGCCCAG TGCCATGAGC CTGCTTGCCC CACCATCCAG CCGCAGAAAG 
GAGTCGTCAA CTCCAGAGGA ATTCAGCCGG CGTCTGAAAG AGCGCATGCA CCACAACATC 
CCTCACCGGT TTAATGTGGG CCTGAACATG AGAGCCACCA AGTGCGCCGT GTGTCTGGAT 
ACTGTGCACT TTGGACGCCA GGCATCCAAA TGCCTCGAAT GTCAAGTGAT GTGTCACCCC 
AAATGCTCCA CCTGCTTGCC CGCTACCTGT GGCCTGCCAG CTGAATATGC CACACACTTC 
ACTGAGGCCT TCTGCCGGGA CAAAATGAAC TCCCCGGGGC TCCAGAGCAA GGAGCCTGGC 
AGCAGCTTGC ACCTGGAAGG GTGGATGAAA GTGCCCAGAA ATAACAAACG GGGACAGCAA 
GGCTGGGACA GGAAGTACAT TGTTCTGGAG GGGTCAAAAG TCCTCATCTA TGACAATGAA 
GCCAGAGAAG CTGGACAGAG GCCGGTGGAA GAATTTGAGC TGTGCCTTCC TGACGGGGAC 
GTATCTATTC ATGGTGCCGT TGGTGCTTCA GAACTTGCAA ATACCGCCAA AGCAGATGTC 
CCATACATCC TGAAGATGGA GTCTCACCCA CACACCACCT GCTGGCCTGG GAGAACCCTC 
TACTTGCTAG CACCCAGCTT CCCCGACAAG CAGCGCTGGG TCACCGCCTT AGAATCTGTC 
GTCGCAGGTG GGAGAGTTTC TAGGGAAAAG GCCGAAGCCG ATGCTGCACG TGACCATACT 
TCGAGTGAGC ATCAGCCAGT GTGGGTTGAG AAATTACTTG GAAACTCTCT GCTGAAACTG 
GAGGGCGATG ACCGGCTTGA CATGAACTGC ACCCTGCCCT TCAGTGACCA GGTAGTGCTG 
GTGGGCACGG AGGAAGGCCT CTACGCCCTG AATGTCTTGA AAAACTCCTT AACCCACATC 
CCAGGGATTG GCGCAGTCTT CCAAATTTAC ATCATCAAGG ACCTGGAGAA GCTGCTCATG 
ATAGCAGGGG AAGAGCGGGC TCTGTGCCTG GTGGACGTGA AGAAGGTGAA GCAGTCCCTG 
GCGCAGTCAC ACCTGCCTGC CCAGCCCGAC GTCTCCCCCA ACATATTCGA AGCCGTCAAA 
GGCTGCCACT TGTTCGCTGC TGGCAAGATC GAGAACAGCC TGTGCATCTG CGCCGCTATG 
CCAAGCAAAG TCGTCATCCT CCGCTACAAT GACAACCTCA GCAAGTACTG CATCCGCAAG 
GAGATCGAGA CCTCAGAGCC CTGCAGCTGT ATCCACTTCA CCAACTACAG CATCCTCATT 
GGGACCAACA AATTCTATGA GATCGACATG AAGCAGTACA CGCTTGACGA GTTCCTGGAC 
AAGAACGACC ATTCCTTGGC ACCTGCTGTG TTCGCCTCCT CGTCCAACAG CTTCCCTGTC 
TCCATTGTGC AGGCGAACAG CGCCGGGCAG CGAGAAGAAT ACCTGCTGTG CTTCCACGAA 
TTTGGGGTGT TCGTGGATTC TTACGGAAGA CGTAGCCGCA CAGATGATCT CAAGTGGAGT 
CGCTTACCTC TGGCCTTCGC CTACAGAGAA CCTTATCTGT TTGTGACTCA CTTCAACTCC 
CTGGAAGTCA TTGAGATCCA GGCCAGATCC TCACTGGGGA GCCCTGCCCG AGCATATCTG 
GAAATTCCAA ACCCTCGCTA CCTGGGCCCC GCGATTTCCT CCGGAGCGAT TTACCTGGCC 
TCCTCATACC AGGACAAGTT AAGGGTCATA TGCTGCAAAG GAAACCTCGT GAAGGAGTCA 
GGCACTGAGC AGCACCGGGT GCCCTCCACC TCCCGCAGCA GCCCCAACAA GCGAGGCCCA 
CCAACATACA ACGAGCACAT CACCAAACGC GTGGCCTCCA GCCCGGCGCC ACCGGAAGGC 
CCCAGCCACC CCCGAGAGCC AAGCACACCG CACCGCTACC GAGACAGAGA GGGTCGGACA 
GAGCTGCGCA GGGACAAGTC TCCAGGCCGC CCTCTGGAGC GGGAGAAGTC CCCAGGCCGA 
ATGCTCAGCA CTAGGAGAGA GCGGTCCCCA GGGAGACTGT TTGAAGACAG CAGCAGGGGC 
CGGCTGCCTG CAGGAGCAGT GAGGACCCCA CTGTCCCAGG TTAACAAGGT CTGGGACCAG 
TCTTCAGTAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cit ( XM_006530132.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006530195.1 Mus musculus citron (Cit), transcript variant X3, mRNA.

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CloneID	OMu35774
Clone ID Related Accession (Same CDS sequence)	XM_006530130.3 , XM_006530132.3 , XM_006530133.3 , XM_006530130.4 , XM_006530131.2 , XM_006530131.1 , XM_006530133.4 , XM_006530132.4
Accession Version	XM_006530132.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6372bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	citron Rho-interacting kinase isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000071.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006530132.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTGAAGT TCAAGTATGG TGTGCGGAAC CCGCCGGAGG CCAGTGCCTC CGAGCCCATT 
GCCAGTCGGG CCTCCAGGCT AAATCTCTTC TTCCAGGGGA AACCGCCCCT CATGACTCAA 
CAGCAGATGT CTGCTCTTTC CCGGGAAGGG ATGCTAGACG CCCTCTTCGC TCTCTTTGAA 
GAGTGCAGCC AACCCGCCCT GATGAAGATG AAGCACGTGA GCAGCTTTGT CCAGAAGTAT 
TCCGACACCA TAGCCGAGTT GCGGGAGCTG CAGCCGTCGG CGAGAGACTT CGAAGTTCGA 
AGCCTTGTGG GCTGTGGTCA CTTCGCTGAA GTGCAGGTGG TTAGAGAGAA GGCGACCGGG 
GACGTCTATG CCATGAAAAT CATGAAGAAG AAGGCTTTGC TGGCCCAGGA ACAGGTTTCA 
TTTTTCGAGG AGGAGAGGAA CATATTATCT CGGAGCACGA GTCCTTGGAT CCCCCAGTTA 
CAGTACGCCT TTCAGGACAA AAATAACCTT TACCTGGTCA TGGAATATCA GCCTGGAGGG 
GATTTGCTGT CGCTTCTGAA CAGATACGAG GACCAATTAG ATGAGAGCAT GATCCAGTTT 
TACCTTGCTG AGCTGATTTT GGCTGTCCAC AGCGTGCACC AGATGGGATA TGTGCATCGA 
GACATCAAGC CCGAGAACAT CCTCATCGAC CGGACGGGAC ACATCAAGCT GGTGGATTTT 
GGATCAGCCG CTAAGATGAA TTCAAATAAG GTGGATGCCA AACTCCCCAT TGGGACCCCG 
GATTACATGG CTCCGGAAGT GTTGACCGTG ATGAACGAGG ACCGAAGGGG CACATACGGC 
TTGGACTGTG ACTGGTGGTC TGTCGGAGTT GTTGCTTATG AGATGGTTTA TGGGAAGACC 
CCATTCACAG AGGGAACCTC CGCCCGGACC TTCAACAACA TCATGAACTT CCAGCGGTTT 
TTGAAGTTCC CAGATGACCC CAAAGTTAGC AGTGAGCTCC TTGATCTGCT TCAGAGTCTG 
CTGTGTGTCC AGAAAGAGAG ACTGAAGTTC GAGGGTCTCT GCTGCCACCC TTTCTTTGCC 
AGAACGGACT GGAACAACAT CCGTAACTCT CCTCCCCCCT TCGTCCCCAC CCTCAAGTCT 
GACGATGACA CCTCCAATTT TGATGAACCA GAGAAGAATT CGTGGGTTTC ATCCTCTGTG 
TGCCAGCTGA GCCCCTCGGG CTTCTCAGGC GAAGAGCTGC CGTTTGTGGG ATTTTCGTAC 
AGCAAGGCAC TGGGGTATCT TGGTAGATCT GAGTCTGTCG TGTCGAGTCT GGACTCCCCT 
GCCAAGGTTA GCTCCATGGA AAAGAAACTT CTCATCAAAA GCAAAGAGCT CCAAGACTCC 
CAGGACAAGT GTCACAAGAT GGAGCAGGAA ATGACCCGGT TACATCGCAG AGTGTCAGAG 
GTGGAGGCTG TGCTTAGTCA GAAGGAGGTG GAGCTGAAGG CCTCTGAGAC TCAGAGATCC 
CTCCTGGAGC AGGACCTTGC TACCTACATC ACAGAATGCA GTAGCTTAAA GCGAAGTTTG 
GAGCAAGCGC GGATGGAGGT GTCCCAGGAG GATGACAAAG CTCTGCAGCT TCTCCACGAC 
ATCCGAGAGC AGAGCCGGAA GCTCCAGGAG ATCAAGGAGC AGGAGTACCA GGCTCAGGTG 
GAGGAGATGA GGCTGATGAT GAATCAGCTG GAAGAAGACC TGGTGTCAGC CCGCAGACGC 
AGCGATCTCT ACGAGTCTGA GCTGAGGGAG TCTCGGCTTG CCGCCGAGGA ATTCAAGCGG 
AAGGCAAACG AATGTCAGCA CAAACTGATG AAGGTAGTCA GCCACCCTCC AAGAGGGGAC 
TCCGGGGGCA CGGCCCTGGA TGATCTTCAC AAGATGCAAG GCCACGCTGG CTTAACGAGT 
GCTAAGGACC AAGGGAAGCC TGAAGTGGGA GAATATTCCA AACTGGAGAA GATCAATGCT 
GAGCAGCAGC TGAAGATCCA GGAGCTCCAG GAGAAGCTGG AGAAGGCTGT AAAAGCCAGC 
ACAGAGGCCA CCGAGCTCCT GCAGAATATC CGCCAGGCAA AGGAGCGAGC AGAGCGGGAG 
CTAGAGAAGC TACACAACCG GGAAGACTCC TCCGAGGGCA TCAAAAAGAA GCTGGTGGAA 
GCCGAGGAAC GCCGCCACTC CCTGGAGAAC AAGGTAAAGA GACTAGAGAC CATGGAGCGT 
AGAGAGAACA GACTGAAGGA TGACATCCAG ACAAAGTCCG AACAGATCCA GCAGATGGCT 
GATAAAATTC TGGAGCTGGA GGAGAAACAT CGGGAGGCTC AGGTCTCAGC TCAACATCTA 
GAAGTACACT TGAAACAGAA AGAACAGCAC TACGAGGAAA AGATCAAGGT ATTGGACAAT 
CAGATAAAGA AGGACCTGGC CGACAAGGAG AGCCTGGAGA ACATGATGCA GAGACACGAG 
GAGGAGGCCC ACGAGAAGGG CAAGATCCTC AGCGAGCAGA AGGCGATGAT CAACGCGATG 
GATTCCAAGA TCCGATCCCT GGAGCAGAGG ATCGTGGAGC TGTCGGAAGC CAACAAGCTT 
GCGGCAAACA GCAGTCTCTT CACCCAGAGG AACATGAAGG CCCAGGAAGA GATGATCTCA 
GAACTCAGGC AGCAGAAATT TTACCTGGAG ACGCAGGCCG GAAAGCTGGA GGCCCAGAAC 
CGAAAGCTGG AAGAGCAACT GGAGAAGATC AGCCACCAAG ATCACAGTGA CAAGAGTCGG 
CTGCTGGAGC TGGAAACAAG GCTGAGGGAG GTCAGCCTGG AGCACGAGGA GCAGAAGCTG 
GAGCTGAAGC GGCAGCTCAC GGAGCTGCAG CTGTCCCTGC AGGAGCGCGA GTCCCAGCTG 
ACGGCCCTGC AGGCCGCCCG GGCAGCGCTG GAGAGCCAGC TCCGCCAGGC GAAGACAGAG 
CTGGAGGAGA CAACCGCGGA AGCGGAGGAG GAGATCCAGG CGCTCACGGC ACATCGAGAT 
GAGATCCAGC GCAAATTCGA TGCCCTTCGC AACAGCTGCA CGGTCATCAC CGACCTGGAG 
GAGCAGCTGA ACCAGCTCAC CGAGGACAAC GCCGAGCTCA ACAACCAAAA CTTCTACCTG 
TCCAAACAAC TCGATGAGGC TTCCGGGGCC AATGACGAGA TTGTGCAGCT GCGGAGTGAG 
GTGGACCATC TCCGCCGTGA GATCACGGAG CGGGAGATGC AGCTCACCAG CCAGAAGCAA 
GCACAACTCA GTGCTCCTGA TTTGCAGACG ATGGAGGCTC TGAAGACGAC ATGCACCATG 
CTGGAGGAGC AGGTCCTGGA CCTGGAGGCC CTGAACGACG AGCTGCTGGA GAAGGAGCGC 
CAGTGGGAGG CCTGGCGGAG CGTCCTCGGC GACGAGAAGT CCCAGTTCGA GTGTCGAGTT 
CGAGAGCTAC AGAGGATGCT GGACACCGAG AAGCAGAGCA GGGCTAGGGC CGATCAGCGG 
ATCACCGAGT CGCGCCAGGT GGTGGAGTTG GCGGTGAAGG AACACAAGGC CGAGATTCTT 
GCTCTGCAGC AGGCTCTCAA GGAGCAGAAG CTCAAAGCCG AGAGCCTGTC GGACAAGCTC 
AACGACCTGG AGAAGAAGCA CGCCATGCTG GAGATGAACG CCCGGAGCTT ACAGCAGAAA 
CTAGAGACAG AGCGGGAGCT CAAACAGAGG CTTCTGGAGG AGCAAGCCAA ATTGCAGCAG 
CAGATGGACC TGCAGAAGAA CCACATCTTC AGACTGACGC AAGGGCTGCA GGAGGCGCTG 
GACCGGGCGG ATCTGCTGAA GACAGAAAGG AGCGACCTGG AGTACCAGCT GGAAAACATT 
CAGGTTCTCT ACTCTCACGA GAAAGTGAAA ATGGAAGGCA CAATCTCTCA GCAAACCAAA 
CTCATTGATT TCCTGCAAGC CAAAATGGAC CAGCCTGCTA AAAAGAAAAA GGGTTTATTT 
AGTCGACGGA AAGAGGACCC TGCTTTGCCC ACACAGGTTC CTCTGCAGTA CAATGAGCTG 
AAGCTAGCCC TGGAAAAGGA GAAAGCCCGA TGCGCGGAGC TGGAGGAGGC CCTTCAGAAG 
ACCCGCATCG AACTCCGCTC TGCGCGGGAG GAAGCTGCCC ACCGCAAAGC CACGGACCAC 
CCGCACCCGT CTACGCCAGC CACTGCGAGG CAGCAGATCG CCATGTCTGC CATTGTGCGG 
TCGCCCGAGC ACCAGCCCAG TGCCATGAGC CTGCTTGCCC CACCATCCAG CCGCAGAAAG 
GAGTCGTCAA CTCCAGAGGA ATTCAGCCGG CGTCTGAAAG AGCGCATGCA CCACAACATC 
CCTCACCGGT TTAATGTGGG CCTGAACATG AGAGCCACCA AGTGCGCCGT GTGTCTGGAT 
ACTGTGCACT TTGGACGCCA GGCATCCAAA TGCCTCGAAT GTCAAGTGAT GTGTCACCCC 
AAATGCTCCA CCTGCTTGCC CGCTACCTGT GGCCTGCCAG CTGAATATGC CACACACTTC 
ACTGAGGCCT TCTGCCGGGA CAAAATGAAC TCCCCGGGGC TCCAGAGCAA GGAGCCTGGC 
AGCAGCTTGC ACCTGGAAGG GTGGATGAAA GTGCCCAGAA ATAACAAACG GGGACAGCAA 
GGCTGGGACA GGAAGTACAT TGTTCTGGAG GGGTCAAAAG TCCTCATCTA TGACAATGAA 
GCCAGAGAAG CTGGACAGAG GCCGGTGGAA GAATTTGAGC TGTGCCTTCC TGACGGGGAC 
GTATCTATTC ATGGTGCCGT TGGTGCTTCA GAACTTGCAA ATACCGCCAA AGCAGATGTC 
CCATACATCC TGAAGATGGA GTCTCACCCA CACACCACCT GCTGGCCTGG GAGAACCCTC 
TACTTGCTAG CACCCAGCTT CCCCGACAAG CAGCGCTGGG TCACCGCCTT AGAATCTGTC 
GTCGCAGGTG GGAGAGTTTC TAGGGAAAAG GCCGAAGCCG ATGCTGCACG TGACCATACT 
TCGAGTGAGC ATCAGCCAGT GTGGGTTGAG AAATTACTTG GAAACTCTCT GCTGAAACTG 
GAGGGCGATG ACCGGCTTGA CATGAACTGC ACCCTGCCCT TCAGTGACCA GGTAGTGCTG 
GTGGGCACGG AGGAAGGCCT CTACGCCCTG AATGTCTTGA AAAACTCCTT AACCCACATC 
CCAGGGATTG GCGCAGTCTT CCAAATTTAC ATCATCAAGG ACCTGGAGAA GCTGCTCATG 
ATAGCAGGGG AAGAGCGGGC TCTGTGCCTG GTGGACGTGA AGAAGGTGAA GCAGTCCCTG 
GCGCAGTCAC ACCTGCCTGC CCAGCCCGAC GTCTCCCCCA ACATATTCGA AGCCGTCAAA 
GGCTGCCACT TGTTCGCTGC TGGCAAGATC GAGAACAGCC TGTGCATCTG CGCCGCTATG 
CCAAGCAAAG TCGTCATCCT CCGCTACAAT GACAACCTCA GCAAGTACTG CATCCGCAAG 
GAGATCGAGA CCTCAGAGCC CTGCAGCTGT ATCCACTTCA CCAACTACAG CATCCTCATT 
GGGACCAACA AATTCTATGA GATCGACATG AAGCAGTACA CGCTTGACGA GTTCCTGGAC 
AAGAACGACC ATTCCTTGGC ACCTGCTGTG TTCGCCTCCT CGTCCAACAG CTTCCCTGTC 
TCCATTGTGC AGGCGAACAG CGCCGGGCAG CGAGAAGAAT ACCTGCTGTG CTTCCACGAA 
TTTGGGGTGT TCGTGGATTC TTACGGAAGA CGTAGCCGCA CAGATGATCT CAAGTGGAGT 
CGCTTACCTC TGGCCTTCGC CTACAGAGAA CCTTATCTGT TTGTGACTCA CTTCAACTCC 
CTGGAAGTCA TTGAGATCCA GGCCAGATCC TCACTGGGGA GCCCTGCCCG AGCATATCTG 
GAAATTCCAA ACCCTCGCTA CCTGGGCCCC GCGATTTCCT CCGGAGCGAT TTACCTGGCC 
TCCTCATACC AGGACAAGTT AAGGGTCATA TGCTGCAAAG GAAACCTCGT GAAGGAGTCA 
GGCACTGAGC AGCACCGGGT GCCCTCCACC TCCCGCAGCA GCCCCAACAA GCGAGGCCCA 
CCAACATACA ACGAGCACAT CACCAAACGC GTGGCCTCCA GCCCGGCGCC ACCGGAAGGC 
CCCAGCCACC CCCGAGAGCC AAGCACACCG CACCGCTACC GAGACAGAGA GGGTCGGACA 
GAGCTGCGCA GGGACAAGTC TCCAGGCCGC CCTCTGGAGC GGGAGAAGTC CCCAGGCCGA 
ATGCTCAGCA CTAGGAGAGA GCGGTCCCCA GGGAGACTGT TTGAAGACAG CAGCAGGGGC 
CGGCTGCCTG CAGGAGCAGT GAGGACCCCA CTGTCCCAGG TTAACAAGGT CTGGGACCAG 
TCTTCAGTAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006530195.1
CDS	125..6496
Translation	MLKFKYGVRNPPEASASEPIASRASRLNLFFQGKPPLMTQQQMSALSREGMLDALFALFEECSQPALMKMKHVSSFVQKYSDTIAELRELQPSARDFEVRSLVGCGHFAEVQVVREKATGDVYAMKIMKKKALLAQEQVSFFEEERNILSRSTSPWIPQLQYAFQDKNNLYLVMEYQPGGDLLSLLNRYEDQLDESMIQFYLAELILAVHSVHQMGYVHRDIKPENILIDRTGHIKLVDFGSAAKMNSNKVDAKLPIGTPDYMAPEVLTVMNEDRRGTYGLDCDWWSVGVVAYEMVYGKTPFTEGTSARTFNNIMNFQRFLKFPDDPKVSSELLDLLQSLLCVQKERLKFEGLCCHPFFARTDWNNIRNSPPPFVPTLKSDDDTSNFDEPEKNSWVSSSVCQLSPSGFSGEELPFVGFSYSKALGYLGRSESVVSSLDSPAKVSSMEKKLLIKSKELQDSQDKCHKMEQEMTRLHRRVSEVEAVLSQKEVELKASETQRSLLEQDLATYITECSSLKRSLEQARMEVSQEDDKALQLLHDIREQSRKLQEIKEQEYQAQVEEMRLMMNQLEEDLVSARRRSDLYESELRESRLAAEEFKRKANECQHKLMKVVSHPPRGDSGGTALDDLHKMQGHAGLTSAKDQGKPEVGEYSKLEKINAEQQLKIQELQEKLEKAVKASTEATELLQNIRQAKERAERELEKLHNREDSSEGIKKKLVEAEERRHSLENKVKRLETMERRENRLKDDIQTKSEQIQQMADKILELEEKHREAQVSAQHLEVHLKQKEQHYEEKIKVLDNQIKKDLADKESLENMMQRHEEEAHEKGKILSEQKAMINAMDSKIRSLEQRIVELSEANKLAANSSLFTQRNMKAQEEMISELRQQKFYLETQAGKLEAQNRKLEEQLEKISHQDHSDKSRLLELETRLREVSLEHEEQKLELKRQLTELQLSLQERESQLTALQAARAALESQLRQAKTELEETTAEAEEEIQALTAHRDEIQRKFDALRNSCTVITDLEEQLNQLTEDNAELNNQNFYLSKQLDEASGANDEIVQLRSEVDHLRREITEREMQLTSQKQAQLSAPDLQTMEALKTTCTMLEEQVLDLEALNDELLEKERQWEAWRSVLGDEKSQFECRVRELQRMLDTEKQSRARADQRITESRQVVELAVKEHKAEILALQQALKEQKLKAESLSDKLNDLEKKHAMLEMNARSLQQKLETERELKQRLLEEQAKLQQQMDLQKNHIFRLTQGLQEALDRADLLKTERSDLEYQLENIQVLYSHEKVKMEGTISQQTKLIDFLQAKMDQPAKKKKGLFSRRKEDPALPTQVPLQYNELKLALEKEKARCAELEEALQKTRIELRSAREEAAHRKATDHPHPSTPATARQQIAMSAIVRSPEHQPSAMSLLAPPSSRRKESSTPEEFSRRLKERMHHNIPHRFNVGLNMRATKCAVCLDTVHFGRQASKCLECQVMCHPKCSTCLPATCGLPAEYATHFTEAFCRDKMNSPGLQSKEPGSSLHLEGWMKVPRNNKRGQQGWDRKYIVLEGSKVLIYDNEAREAGQRPVEEFELCLPDGDVSIHGAVGASELANTAKADVPYILKMESHPHTTCWPGRTLYLLAPSFPDKQRWVTALESVVAGGRVSREKAEADAARDHTSSEHQPVWVEKLLGNSLLKLEGDDRLDMNCTLPFSDQVVLVGTEEGLYALNVLKNSLTHIPGIGAVFQIYIIKDLEKLLMIAGEERALCLVDVKKVKQSLAQSHLPAQPDVSPNIFEAVKGCHLFAAGKIENSLCICAAMPSKVVILRYNDNLSKYCIRKEIETSEPCSCIHFTNYSILIGTNKFYEIDMKQYTLDEFLDKNDHSLAPAVFASSSNSFPVSIVQANSAGQREEYLLCFHEFGVFVDSYGRRSRTDDLKWSRLPLAFAYREPYLFVTHFNSLEVIEIQARSSLGSPARAYLEIPNPRYLGPAISSGAIYLASSYQDKLRVICCKGNLVKESGTEQHRVPSTSRSSPNKRGPPTYNEHITKRVASSPAPPEGPSHPREPSTPHRYRDREGRTELRRDKSPGRPLEREKSPGRMLSTRRERSPGRLFEDSSRGRLPAGAVRTPLSQVNKVWDQSSV

Target ORF information:

RefSeq Version	XM_006530132.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus citron (Cit), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006530132.4

ORF Insert Sequence:

ATGTTGAAGT TCAAGTATGG TGTGCGGAAC CCGCCGGAGG CCAGTGCCTC CGAGCCCATT 
GCCAGTCGGG CCTCCAGGCT AAATCTCTTC TTCCAGGGGA AACCGCCCCT CATGACTCAA 
CAGCAGATGT CTGCTCTTTC CCGGGAAGGG ATGCTAGACG CCCTCTTCGC TCTCTTTGAA 
GAGTGCAGCC AACCCGCCCT GATGAAGATG AAGCACGTGA GCAGCTTTGT CCAGAAGTAT 
TCCGACACCA TAGCCGAGTT GCGGGAGCTG CAGCCGTCGG CGAGAGACTT CGAAGTTCGA 
AGCCTTGTGG GCTGTGGTCA CTTCGCTGAA GTGCAGGTGG TTAGAGAGAA GGCGACCGGG 
GACGTCTATG CCATGAAAAT CATGAAGAAG AAGGCTTTGC TGGCCCAGGA ACAGGTTTCA 
TTTTTCGAGG AGGAGAGGAA CATATTATCT CGGAGCACGA GTCCTTGGAT CCCCCAGTTA 
CAGTACGCCT TTCAGGACAA AAATAACCTT TACCTGGTCA TGGAATATCA GCCTGGAGGG 
GATTTGCTGT CGCTTCTGAA CAGATACGAG GACCAATTAG ATGAGAGCAT GATCCAGTTT 
TACCTTGCTG AGCTGATTTT GGCTGTCCAC AGCGTGCACC AGATGGGATA TGTGCATCGA 
GACATCAAGC CCGAGAACAT CCTCATCGAC CGGACGGGAC ACATCAAGCT GGTGGATTTT 
GGATCAGCCG CTAAGATGAA TTCAAATAAG GTGGATGCCA AACTCCCCAT TGGGACCCCG 
GATTACATGG CTCCGGAAGT GTTGACCGTG ATGAACGAGG ACCGAAGGGG CACATACGGC 
TTGGACTGTG ACTGGTGGTC TGTCGGAGTT GTTGCTTATG AGATGGTTTA TGGGAAGACC 
CCATTCACAG AGGGAACCTC CGCCCGGACC TTCAACAACA TCATGAACTT CCAGCGGTTT 
TTGAAGTTCC CAGATGACCC CAAAGTTAGC AGTGAGCTCC TTGATCTGCT TCAGAGTCTG 
CTGTGTGTCC AGAAAGAGAG ACTGAAGTTC GAGGGTCTCT GCTGCCACCC TTTCTTTGCC 
AGAACGGACT GGAACAACAT CCGTAACTCT CCTCCCCCCT TCGTCCCCAC CCTCAAGTCT 
GACGATGACA CCTCCAATTT TGATGAACCA GAGAAGAATT CGTGGGTTTC ATCCTCTGTG 
TGCCAGCTGA GCCCCTCGGG CTTCTCAGGC GAAGAGCTGC CGTTTGTGGG ATTTTCGTAC 
AGCAAGGCAC TGGGGTATCT TGGTAGATCT GAGTCTGTCG TGTCGAGTCT GGACTCCCCT 
GCCAAGGTTA GCTCCATGGA AAAGAAACTT CTCATCAAAA GCAAAGAGCT CCAAGACTCC 
CAGGACAAGT GTCACAAGAT GGAGCAGGAA ATGACCCGGT TACATCGCAG AGTGTCAGAG 
GTGGAGGCTG TGCTTAGTCA GAAGGAGGTG GAGCTGAAGG CCTCTGAGAC TCAGAGATCC 
CTCCTGGAGC AGGACCTTGC TACCTACATC ACAGAATGCA GTAGCTTAAA GCGAAGTTTG 
GAGCAAGCGC GGATGGAGGT GTCCCAGGAG GATGACAAAG CTCTGCAGCT TCTCCACGAC 
ATCCGAGAGC AGAGCCGGAA GCTCCAGGAG ATCAAGGAGC AGGAGTACCA GGCTCAGGTG 
GAGGAGATGA GGCTGATGAT GAATCAGCTG GAAGAAGACC TGGTGTCAGC CCGCAGACGC 
AGCGATCTCT ACGAGTCTGA GCTGAGGGAG TCTCGGCTTG CCGCCGAGGA ATTCAAGCGG 
AAGGCAAACG AATGTCAGCA CAAACTGATG AAGGTAGTCA GCCACCCTCC AAGAGGGGAC 
TCCGGGGGCA CGGCCCTGGA TGATCTTCAC AAGATGCAAG GCCACGCTGG CTTAACGAGT 
GCTAAGGACC AAGGGAAGCC TGAAGTGGGA GAATATTCCA AACTGGAGAA GATCAATGCT 
GAGCAGCAGC TGAAGATCCA GGAGCTCCAG GAGAAGCTGG AGAAGGCTGT AAAAGCCAGC 
ACAGAGGCCA CCGAGCTCCT GCAGAATATC CGCCAGGCAA AGGAGCGAGC AGAGCGGGAG 
CTAGAGAAGC TACACAACCG GGAAGACTCC TCCGAGGGCA TCAAAAAGAA GCTGGTGGAA 
GCCGAGGAAC GCCGCCACTC CCTGGAGAAC AAGGTAAAGA GACTAGAGAC CATGGAGCGT 
AGAGAGAACA GACTGAAGGA TGACATCCAG ACAAAGTCCG AACAGATCCA GCAGATGGCT 
GATAAAATTC TGGAGCTGGA GGAGAAACAT CGGGAGGCTC AGGTCTCAGC TCAACATCTA 
GAAGTACACT TGAAACAGAA AGAACAGCAC TACGAGGAAA AGATCAAGGT ATTGGACAAT 
CAGATAAAGA AGGACCTGGC CGACAAGGAG AGCCTGGAGA ACATGATGCA GAGACACGAG 
GAGGAGGCCC ACGAGAAGGG CAAGATCCTC AGCGAGCAGA AGGCGATGAT CAACGCGATG 
GATTCCAAGA TCCGATCCCT GGAGCAGAGG ATCGTGGAGC TGTCGGAAGC CAACAAGCTT 
GCGGCAAACA GCAGTCTCTT CACCCAGAGG AACATGAAGG CCCAGGAAGA GATGATCTCA 
GAACTCAGGC AGCAGAAATT TTACCTGGAG ACGCAGGCCG GAAAGCTGGA GGCCCAGAAC 
CGAAAGCTGG AAGAGCAACT GGAGAAGATC AGCCACCAAG ATCACAGTGA CAAGAGTCGG 
CTGCTGGAGC TGGAAACAAG GCTGAGGGAG GTCAGCCTGG AGCACGAGGA GCAGAAGCTG 
GAGCTGAAGC GGCAGCTCAC GGAGCTGCAG CTGTCCCTGC AGGAGCGCGA GTCCCAGCTG 
ACGGCCCTGC AGGCCGCCCG GGCAGCGCTG GAGAGCCAGC TCCGCCAGGC GAAGACAGAG 
CTGGAGGAGA CAACCGCGGA AGCGGAGGAG GAGATCCAGG CGCTCACGGC ACATCGAGAT 
GAGATCCAGC GCAAATTCGA TGCCCTTCGC AACAGCTGCA CGGTCATCAC CGACCTGGAG 
GAGCAGCTGA ACCAGCTCAC CGAGGACAAC GCCGAGCTCA ACAACCAAAA CTTCTACCTG 
TCCAAACAAC TCGATGAGGC TTCCGGGGCC AATGACGAGA TTGTGCAGCT GCGGAGTGAG 
GTGGACCATC TCCGCCGTGA GATCACGGAG CGGGAGATGC AGCTCACCAG CCAGAAGCAA 
GCACAACTCA GTGCTCCTGA TTTGCAGACG ATGGAGGCTC TGAAGACGAC ATGCACCATG 
CTGGAGGAGC AGGTCCTGGA CCTGGAGGCC CTGAACGACG AGCTGCTGGA GAAGGAGCGC 
CAGTGGGAGG CCTGGCGGAG CGTCCTCGGC GACGAGAAGT CCCAGTTCGA GTGTCGAGTT 
CGAGAGCTAC AGAGGATGCT GGACACCGAG AAGCAGAGCA GGGCTAGGGC CGATCAGCGG 
ATCACCGAGT CGCGCCAGGT GGTGGAGTTG GCGGTGAAGG AACACAAGGC CGAGATTCTT 
GCTCTGCAGC AGGCTCTCAA GGAGCAGAAG CTCAAAGCCG AGAGCCTGTC GGACAAGCTC 
AACGACCTGG AGAAGAAGCA CGCCATGCTG GAGATGAACG CCCGGAGCTT ACAGCAGAAA 
CTAGAGACAG AGCGGGAGCT CAAACAGAGG CTTCTGGAGG AGCAAGCCAA ATTGCAGCAG 
CAGATGGACC TGCAGAAGAA CCACATCTTC AGACTGACGC AAGGGCTGCA GGAGGCGCTG 
GACCGGGCGG ATCTGCTGAA GACAGAAAGG AGCGACCTGG AGTACCAGCT GGAAAACATT 
CAGGTTCTCT ACTCTCACGA GAAAGTGAAA ATGGAAGGCA CAATCTCTCA GCAAACCAAA 
CTCATTGATT TCCTGCAAGC CAAAATGGAC CAGCCTGCTA AAAAGAAAAA GGGTTTATTT 
AGTCGACGGA AAGAGGACCC TGCTTTGCCC ACACAGGTTC CTCTGCAGTA CAATGAGCTG 
AAGCTAGCCC TGGAAAAGGA GAAAGCCCGA TGCGCGGAGC TGGAGGAGGC CCTTCAGAAG 
ACCCGCATCG AACTCCGCTC TGCGCGGGAG GAAGCTGCCC ACCGCAAAGC CACGGACCAC 
CCGCACCCGT CTACGCCAGC CACTGCGAGG CAGCAGATCG CCATGTCTGC CATTGTGCGG 
TCGCCCGAGC ACCAGCCCAG TGCCATGAGC CTGCTTGCCC CACCATCCAG CCGCAGAAAG 
GAGTCGTCAA CTCCAGAGGA ATTCAGCCGG CGTCTGAAAG AGCGCATGCA CCACAACATC 
CCTCACCGGT TTAATGTGGG CCTGAACATG AGAGCCACCA AGTGCGCCGT GTGTCTGGAT 
ACTGTGCACT TTGGACGCCA GGCATCCAAA TGCCTCGAAT GTCAAGTGAT GTGTCACCCC 
AAATGCTCCA CCTGCTTGCC CGCTACCTGT GGCCTGCCAG CTGAATATGC CACACACTTC 
ACTGAGGCCT TCTGCCGGGA CAAAATGAAC TCCCCGGGGC TCCAGAGCAA GGAGCCTGGC 
AGCAGCTTGC ACCTGGAAGG GTGGATGAAA GTGCCCAGAA ATAACAAACG GGGACAGCAA 
GGCTGGGACA GGAAGTACAT TGTTCTGGAG GGGTCAAAAG TCCTCATCTA TGACAATGAA 
GCCAGAGAAG CTGGACAGAG GCCGGTGGAA GAATTTGAGC TGTGCCTTCC TGACGGGGAC 
GTATCTATTC ATGGTGCCGT TGGTGCTTCA GAACTTGCAA ATACCGCCAA AGCAGATGTC 
CCATACATCC TGAAGATGGA GTCTCACCCA CACACCACCT GCTGGCCTGG GAGAACCCTC 
TACTTGCTAG CACCCAGCTT CCCCGACAAG CAGCGCTGGG TCACCGCCTT AGAATCTGTC 
GTCGCAGGTG GGAGAGTTTC TAGGGAAAAG GCCGAAGCCG ATGCTGCACG TGACCATACT 
TCGAGTGAGC ATCAGCCAGT GTGGGTTGAG AAATTACTTG GAAACTCTCT GCTGAAACTG 
GAGGGCGATG ACCGGCTTGA CATGAACTGC ACCCTGCCCT TCAGTGACCA GGTAGTGCTG 
GTGGGCACGG AGGAAGGCCT CTACGCCCTG AATGTCTTGA AAAACTCCTT AACCCACATC 
CCAGGGATTG GCGCAGTCTT CCAAATTTAC ATCATCAAGG ACCTGGAGAA GCTGCTCATG 
ATAGCAGGGG AAGAGCGGGC TCTGTGCCTG GTGGACGTGA AGAAGGTGAA GCAGTCCCTG 
GCGCAGTCAC ACCTGCCTGC CCAGCCCGAC GTCTCCCCCA ACATATTCGA AGCCGTCAAA 
GGCTGCCACT TGTTCGCTGC TGGCAAGATC GAGAACAGCC TGTGCATCTG CGCCGCTATG 
CCAAGCAAAG TCGTCATCCT CCGCTACAAT GACAACCTCA GCAAGTACTG CATCCGCAAG 
GAGATCGAGA CCTCAGAGCC CTGCAGCTGT ATCCACTTCA CCAACTACAG CATCCTCATT 
GGGACCAACA AATTCTATGA GATCGACATG AAGCAGTACA CGCTTGACGA GTTCCTGGAC 
AAGAACGACC ATTCCTTGGC ACCTGCTGTG TTCGCCTCCT CGTCCAACAG CTTCCCTGTC 
TCCATTGTGC AGGCGAACAG CGCCGGGCAG CGAGAAGAAT ACCTGCTGTG CTTCCACGAA 
TTTGGGGTGT TCGTGGATTC TTACGGAAGA CGTAGCCGCA CAGATGATCT CAAGTGGAGT 
CGCTTACCTC TGGCCTTCGC CTACAGAGAA CCTTATCTGT TTGTGACTCA CTTCAACTCC 
CTGGAAGTCA TTGAGATCCA GGCCAGATCC TCACTGGGGA GCCCTGCCCG AGCATATCTG 
GAAATTCCAA ACCCTCGCTA CCTGGGCCCC GCGATTTCCT CCGGAGCGAT TTACCTGGCC 
TCCTCATACC AGGACAAGTT AAGGGTCATA TGCTGCAAAG GAAACCTCGT GAAGGAGTCA 
GGCACTGAGC AGCACCGGGT GCCCTCCACC TCCCGCAGCA GCCCCAACAA GCGAGGCCCA 
CCAACATACA ACGAGCACAT CACCAAACGC GTGGCCTCCA GCCCGGCGCC ACCGGAAGGC 
CCCAGCCACC CCCGAGAGCC AAGCACACCG CACCGCTACC GAGACAGAGA GGGTCGGACA 
GAGCTGCGCA GGGACAAGTC TCCAGGCCGC CCTCTGGAGC GGGAGAAGTC CCCAGGCCGA 
ATGCTCAGCA CTAGGAGAGA GCGGTCCCCA GGGAGACTGT TTGAAGACAG CAGCAGGGGC 
CGGCTGCCTG CAGGAGCAGT GAGGACCCCA CTGTCCCAGG TTAACAAGGT CTGGGACCAG 
TCTTCAGTAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cit ( XM_006530134.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006530197.1 Mus musculus citron (Cit), transcript variant X5, mRNA.

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CloneID	OMu35775
Clone ID Related Accession (Same CDS sequence)	XM_006530134.3 , XM_006530134.4
Accession Version	XM_006530134.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6369bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	citron Rho-interacting kinase isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000071.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006530134.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTGAAGT TCAAGTATGG TGTGCGGAAC CCGCCGGAGG CCAGTGCCTC CGAGCCCATT 
GCCAGTCGGG CCTCCAGGCT AAATCTCTTC TTCCAGGGGA AACCGCCCCT CATGACTCAA 
CAGCAGATGT CTGCTCTTTC CCGGGAAGGG ATGCTAGACG CCCTCTTCGC TCTCTTTGAA 
GAGTGCAGCC AACCCGCCCT GATGAAGATG AAGCACGTGA GCAGCTTTGT CCAGAAGTAT 
TCCGACACCA TAGCCGAGTT GCGGGAGCTG CAGCCGTCGG CGAGAGACTT CGAAGTTCGA 
AGCCTTGTGG GCTGTGGTCA CTTCGCTGAA GTGCAGGTGG TTAGAGAGAA GGCGACCGGG 
GACGTCTATG CCATGAAAAT CATGAAGAAG AAGGCTTTGC TGGCCCAGGA ACAGGTTTCA 
TTTTTCGAGG AGGAGAGGAA CATATTATCT CGGAGCACGA GTCCTTGGAT CCCCCAGTTA 
CAGTACGCCT TTCAGGACAA AAATAACCTT TACCTGGTCA TGGAATATCA GCCTGGAGGG 
GATTTGCTGT CGCTTCTGAA CAGATACGAG GACCAATTAG ATGAGAGCAT GATCCAGTTT 
TACCTTGCTG AGCTGATTTT GGCTGTCCAC AGCGTGCACC AGATGGGATA TGTGCATCGA 
GACATCAAGC CCGAGAACAT CCTCATCGAC CGGACGGGAC ACATCAAGCT GGTGGATTTT 
GGATCAGCCG CTAAGATGAA TTCAAATAAG GTGGATGCCA AACTCCCCAT TGGGACCCCG 
GATTACATGG CTCCGGAAGT GTTGACCGTG ATGAACGAGG ACCGAAGGGG CACATACGGC 
TTGGACTGTG ACTGGTGGTC TGTCGGAGTT GTTGCTTATG AGATGGTTTA TGGGAAGACC 
CCATTCACAG AGGGAACCTC CGCCCGGACC TTCAACAACA TCATGAACTT CCAGCGGTTT 
TTGAAGTTCC CAGATGACCC CAAAGTTAGC AGTGAGCTCC TTGATCTGCT TCAGAGTCTG 
CTGTGTGTCC AGAAAGAGAG ACTGAAGTTC GAGGGTCTCT GCTGCCACCC TTTCTTTGCC 
AGAACGGACT GGAACAACAT CCGTAACTCT CCTCCCCCCT TCGTCCCCAC CCTCAAGTCT 
GACGATGACA CCTCCAATTT TGATGAACCA GAGAAGAATT CGTGGGTTTC ATCCTCTGTG 
TGCCAGCTGA GCCCCTCGGG CTTCTCAGGC GAAGAGCTGC CGTTTGTGGG ATTTTCGTAC 
AGCAAGGCAC TGGGGTATCT TGGTAGATCT GAGTCTGTCG TGTCGAGTCT GGACTCCCCT 
GCCAAGGTTA GCTCCATGGA AAAGAAACTT CTCATCAAAA GCAAAGAGCT CCAAGACTCC 
CAGGACAAGT GTCACAAGAT GGAGCAGGAA ATGACCCGGT TACATCGCAG AGTGTCAGAG 
GTGGAGGCTG TGCTTAGTCA GAAGGAGGTG GAGCTGAAGG CCTCTGAGAC TCAGAGATCC 
CTCCTGGAGC AGGACCTTGC TACCTACATC ACAGAATGCA GTAGCTTAAA GCGAAGTTTG 
GAGCAAGCGC GGATGGAGGT GTCCCAGGAG GATGACAAAG CTCTGCAGCT TCTCCACGAC 
ATCCGAGAGC AGAGCCGGAA GCTCCAGGAG ATCAAGGAGC AGGAGTACCA GGCTCAGGTG 
GAGGAGATGA GGCTGATGAT GAATCAGCTG GAAGAAGACC TGGTGTCAGC CCGCAGACGC 
AGCGATCTCT ACGAGTCTGA GCTGAGGGAG TCTCGGCTTG CCGCCGAGGA ATTCAAGCGG 
AAGGCAAACG AATGTCAGCA CAAACTGATG AAGGTAGTCA GCCACCCTCC AAGAGGGGAC 
TCCGGGGGCA CGGCCCTGGA TGATCTTCAC AAGATGCAAG GCCACGCTGG CTTAACGAGT 
GCTAAGGACC AAGGGAAGCC TGAAGTGGGA GAATATTCCA AACTGGAGAA GATCAATGCT 
GAGCAGCAGC TGAAGATCCA GGAGCTCCAG GAGAAGCTGG AGAAGGCTGT AAAAGCCAGC 
ACAGAGGCCA CCGAGCTCCT GCAGAATATC CGCCAGGCAA AGGAGCGAGC AGAGCGGGAG 
CTAGAGAAGC TACACAACCG GGAAGACTCC TCCGAGGGCA TCAAAAAGAA GCTGGTGGAA 
GCCGAGGAAC GCCGCCACTC CCTGGAGAAC AAGGTAAAGA GACTAGAGAC CATGGAGCGT 
AGAGAGAACA GACTGAAGGA TGACATCCAG ACAAAGTCCG AACAGATCCA GCAGATGGCT 
GATAAAATTC TGGAGCTGGA GGAGAAACAT CGGGAGGCTC AGGTCTCAGC TCAACATCTA 
GAAGTACACT TGAAACAGAA AGAACAGCAC TACGAGGAAA AGATCAAGGT ATTGGACAAT 
CAGATAAAGA AGGACCTGGC CGACAAGGAG AGCCTGGAGA ACATGATGCA GAGACACGAG 
GAGGAGGCCC ACGAGAAGGG CAAGATCCTC AGCGAGCAGA AGGCGATGAT CAACGCGATG 
GATTCCAAGA TCCGATCCCT GGAGCAGAGG ATCGTGGAGC TGTCGGAAGC CAACAAGCTT 
GCGGCAAACA GCAGTCTCTT CACCCAGAGG AACATGAAGG CCCAGGAAGA GATGATCTCA 
GAACTCAGGC AGCAGAAATT TTACCTGGAG ACGCAGGCCG GAAAGCTGGA GGCCCAGAAC 
CGAAAGCTGG AAGAGCAACT GGAGAAGATC AGCCACCAAG ATCACAGTGA CAAGAGTCGG 
CTGCTGGAGC TGGAAACAAG GCTGAGGGAG GTCAGCCTGG AGCACGAGGA GCAGAAGCTG 
GAGCTGAAGC GGCAGCTCAC GGAGCTGCAG CTGTCCCTGC AGGAGCGCGA GTCCCAGCTG 
ACGGCCCTGC AGGCCGCCCG GGCAGCGCTG GAGAGCCAGC TCCGCCAGGC GAAGACAGAG 
CTGGAGGAGA CAACCGCGGA AGCGGAGGAG GAGATCCAGG CGCTCACGGC ACATCGAGAT 
GAGATCCAGC GCAAATTCGA TGCCCTTCGC AACAGCTGCA CGGTCATCAC CGACCTGGAG 
GAGCAGCTGA ACCAGCTCAC CGAGGACAAC GCCGAGCTCA ACAACCAAAA CTTCTACCTG 
TCCAAACAAC TCGATGAGGC TTCCGGGGCC AATGACGAGA TTGTGCAGCT GCGGAGTGAG 
GTGGACCATC TCCGCCGTGA GATCACGGAG CGGGAGATGC AGCTCACCAG CCAGAAGCAA 
GCACAACTCA GTGCTCCTGA TTTGCAGACG ATGGAGGCTC TGAAGACGAC ATGCACCATG 
CTGGAGGAGC AGGTCCTGGA CCTGGAGGCC CTGAACGACG AGCTGCTGGA GAAGGAGCGC 
CAGTGGGAGG CCTGGCGGAG CGTCCTCGGC GACGAGAAGT CCCAGTTCGA GTGTCGAGTT 
CGAGAGCTAC AGAGGATGCT GGACACCGAG AAGCAGAGCA GGGCTAGGGC CGATCAGCGG 
ATCACCGAGT CGCGCCAGGT GGTGGAGTTG GCGGTGAAGG AACACAAGGC CGAGATTCTT 
GCTCTGCAGC AGGCTCTCAA GGAGCAGAAG CTCAAAGCCG AGAGCCTGTC GGACAAGCTC 
AACGACCTGG AGAAGAAGCA CGCCATGCTG GAGATGAACG CCCGGAGCTT ACAGCAGAAA 
CTAGAGACAG AGCGGGAGCT CAAACAGAGG CTTCTGGAGG AGCAAGCCAA ATTGCAGCAG 
CAGATGGACC TGCAGAAGAA CCACATCTTC AGACTGACGC AAGGGCTGCA GGAGGCGCTG 
GACCGGGCGG ATCTGCTGAA GACAGAAAGG AGCGACCTGG AGTACCAGCT GGAAAACATT 
CAGGTTCTCT ACTCTCACGA GAAAGTGAAA ATGGAAGGCA CAATCTCTCA GCAAACCAAA 
CTCATTGATT TCCTGCAAGC CAAAATGGAC CAGCCTGCTA AAAAGAAAAA GGGTTTATTT 
AGTCGACGGA AAGAGGACCC TGCTTTGCCC ACACAGGTTC CTCTGCAGTA CAATGAGCTG 
AAGCTAGCCC TGGAAAAGGA GAAAGCCCGA TGCGCGGAGC TGGAGGAGGC CCTTCAGAAG 
ACCCGCATCG AACTCCGCTC TGCGCGGGAG GAAGCTGCCC ACCGCAAAGC CACGGACCAC 
CCGCACCCGT CTACGCCAGC CACTGCGAGG CAGCAGATCG CCATGTCTGC CATTGTGCGG 
TCGCCCGAGC ACCAGCCCAG TGCCATGAGC CTGCTTGCCC CACCATCCAG CCGCAGAAAG 
GAGTCGTCAA CTCCAGAGGA ATTCAGCCGG CGTCTGAAAG AGCGCATGCA CCACAACATC 
CCTCACCGGT TTAATGTGGG CCTGAACATG AGAGCCACCA AGTGCGCCGT GTGTCTGGAT 
ACTGTGCACT TTGGACGCCA GGCATCCAAA TGCCTCGAAT GTCAAGTGAT GTGTCACCCC 
AAATGCTCCA CCTGCTTGCC CGCTACCTGT GGCCTGCCAG CTGAATATGC CACACACTTC 
ACTGAGGCCT TCTGCCGGGA CAAAATGAAC TCCCCGGGGC TCCAGAGCAA GGAGCCTGGC 
AGCAGCTTGC ACCTGGAAGG GTGGATGAAA GTGCCCAGAA ATAACAAACG GGGACAGCAA 
GGCTGGGACA GGAAGTACAT TGTTCTGGAG GGGTCAAAAG TCCTCATCTA TGACAATGAA 
GCCAGAGAAG CTGGACAGAG GCCGGTGGAA GAATTTGAGC TGTGCCTTCC TGACGGGGAC 
GTATCTATTC ATGGTGCCGT TGGTGCTTCA GAACTTGCAA ATACCGCCAA AGCAGATGTC 
CCATACATCC TGAAGATGGA GTCTCACCCA CACACCACCT GCTGGCCTGG GAGAACCCTC 
TACTTGCTAG CACCCAGCTT CCCCGACAAG CAGCGCTGGG TCACCGCCTT AGAATCTGTC 
GTCGCAGGTG GGAGAGTTTC TAGGGAAAAG GCCGAAGCCG ATGCTGCACG TGACCATACT 
TCGAGTGAGC ATCAGCCAGT GTGGGTTGAG AAATTACTTG GAAACTCTCT GCTGAAACTG 
GAGGGCGATG ACCGGCTTGA CATGAACTGC ACCCTGCCCT TCAGTGACCA GGTAGTGCTG 
GTGGGCACGG AGGAAGGCCT CTACGCCCTG AATGTCTTGA AAAACTCCTT AACCCACATC 
CCAGGGATTG GCGCAGTCTT CCAAATTTAC ATCATCAAGG ACCTGGAGAA GCTGCTCATG 
ATAGCAGGGG AAGAGCGGGC TCTGTGCCTG GTGGACGTGA AGAAGGTGAA GCAGTCCCTG 
GCGCAGTCAC ACCTGCCTGC CCAGCCCGAC GTCTCCCCCA ACATATTCGA AGCCGTCAAA 
GGCTGCCACT TGTTCGCTGC TGGCAAGATC GAGAACAGCC TGTGCATCTG CGCCGCTATG 
CCAAGCAAAG TCGTCATCCT CCGCTACAAT GACAACCTCA GCAAGTACTG CATCCGCAAG 
GAGATCGAGA CCTCAGAGCC CTGCAGCTGT ATCCACTTCA CCAACTACAG CATCCTCATT 
GGGACCAACA AATTCTATGA GATCGACATG AAGCAGTACA CGCTTGACGA GTTCCTGGAC 
AAGAACGACC ATTCCTTGGC ACCTGCTGTG TTCGCCTCCT CGTCCAACAG CTTCCCTGTC 
TCCATTGTGC AGGCGAACAG CGCCGGGCAG CGAGAAGAAT ACCTGCTGTG CTTCCACGAA 
TTTGGGGTGT TCGTGGATTC TTACGGAAGA CGTAGCCGCA CAGATGATCT CAAGTGGAGT 
CGCTTACCTC TGGCCTTCGC CTACAGAGAA CCTTATCTGT TTGTGACTCA CTTCAACTCC 
CTGGAAGTCA TTGAGATCCA GGCCAGATCC TCACTGGGGA GCCCTGCCCG AGCATATCTG 
GAAATTCCAA ACCCTCGCTA CCTGGGCCCC GCGATTTCCT CCGGAGCGAT TTACCTGGCC 
TCCTCATACC AGGACAAGTT AAGGGTCATA TGCTGCAAAG GAAACCTCGT GAAGGAGTCA 
GGCACTGAGC AGCACCGGGT GCCCTCCACC TCCCGCAGCC CCAACAAGCG AGGCCCACCA 
ACATACAACG AGCACATCAC CAAACGCGTG GCCTCCAGCC CGGCGCCACC GGAAGGCCCC 
AGCCACCCCC GAGAGCCAAG CACACCGCAC CGCTACCGAG ACAGAGAGGG TCGGACAGAG 
CTGCGCAGGG ACAAGTCTCC AGGCCGCCCT CTGGAGCGGG AGAAGTCCCC AGGCCGAATG 
CTCAGCACTA GGAGAGAGCG GTCCCCAGGG AGACTGTTTG AAGACAGCAG CAGGGGCCGG 
CTGCCTGCAG GAGCAGTGAG GACCCCACTG TCCCAGGTTA ACAAGGTCTG GGACCAGTCT 
TCAGTATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006530197.1
CDS	158..6526
Translation	MLKFKYGVRNPPEASASEPIASRASRLNLFFQGKPPLMTQQQMSALSREGMLDALFALFEECSQPALMKMKHVSSFVQKYSDTIAELRELQPSARDFEVRSLVGCGHFAEVQVVREKATGDVYAMKIMKKKALLAQEQVSFFEEERNILSRSTSPWIPQLQYAFQDKNNLYLVMEYQPGGDLLSLLNRYEDQLDESMIQFYLAELILAVHSVHQMGYVHRDIKPENILIDRTGHIKLVDFGSAAKMNSNKVDAKLPIGTPDYMAPEVLTVMNEDRRGTYGLDCDWWSVGVVAYEMVYGKTPFTEGTSARTFNNIMNFQRFLKFPDDPKVSSELLDLLQSLLCVQKERLKFEGLCCHPFFARTDWNNIRNSPPPFVPTLKSDDDTSNFDEPEKNSWVSSSVCQLSPSGFSGEELPFVGFSYSKALGYLGRSESVVSSLDSPAKVSSMEKKLLIKSKELQDSQDKCHKMEQEMTRLHRRVSEVEAVLSQKEVELKASETQRSLLEQDLATYITECSSLKRSLEQARMEVSQEDDKALQLLHDIREQSRKLQEIKEQEYQAQVEEMRLMMNQLEEDLVSARRRSDLYESELRESRLAAEEFKRKANECQHKLMKVVSHPPRGDSGGTALDDLHKMQGHAGLTSAKDQGKPEVGEYSKLEKINAEQQLKIQELQEKLEKAVKASTEATELLQNIRQAKERAERELEKLHNREDSSEGIKKKLVEAEERRHSLENKVKRLETMERRENRLKDDIQTKSEQIQQMADKILELEEKHREAQVSAQHLEVHLKQKEQHYEEKIKVLDNQIKKDLADKESLENMMQRHEEEAHEKGKILSEQKAMINAMDSKIRSLEQRIVELSEANKLAANSSLFTQRNMKAQEEMISELRQQKFYLETQAGKLEAQNRKLEEQLEKISHQDHSDKSRLLELETRLREVSLEHEEQKLELKRQLTELQLSLQERESQLTALQAARAALESQLRQAKTELEETTAEAEEEIQALTAHRDEIQRKFDALRNSCTVITDLEEQLNQLTEDNAELNNQNFYLSKQLDEASGANDEIVQLRSEVDHLRREITEREMQLTSQKQAQLSAPDLQTMEALKTTCTMLEEQVLDLEALNDELLEKERQWEAWRSVLGDEKSQFECRVRELQRMLDTEKQSRARADQRITESRQVVELAVKEHKAEILALQQALKEQKLKAESLSDKLNDLEKKHAMLEMNARSLQQKLETERELKQRLLEEQAKLQQQMDLQKNHIFRLTQGLQEALDRADLLKTERSDLEYQLENIQVLYSHEKVKMEGTISQQTKLIDFLQAKMDQPAKKKKGLFSRRKEDPALPTQVPLQYNELKLALEKEKARCAELEEALQKTRIELRSAREEAAHRKATDHPHPSTPATARQQIAMSAIVRSPEHQPSAMSLLAPPSSRRKESSTPEEFSRRLKERMHHNIPHRFNVGLNMRATKCAVCLDTVHFGRQASKCLECQVMCHPKCSTCLPATCGLPAEYATHFTEAFCRDKMNSPGLQSKEPGSSLHLEGWMKVPRNNKRGQQGWDRKYIVLEGSKVLIYDNEAREAGQRPVEEFELCLPDGDVSIHGAVGASELANTAKADVPYILKMESHPHTTCWPGRTLYLLAPSFPDKQRWVTALESVVAGGRVSREKAEADAARDHTSSEHQPVWVEKLLGNSLLKLEGDDRLDMNCTLPFSDQVVLVGTEEGLYALNVLKNSLTHIPGIGAVFQIYIIKDLEKLLMIAGEERALCLVDVKKVKQSLAQSHLPAQPDVSPNIFEAVKGCHLFAAGKIENSLCICAAMPSKVVILRYNDNLSKYCIRKEIETSEPCSCIHFTNYSILIGTNKFYEIDMKQYTLDEFLDKNDHSLAPAVFASSSNSFPVSIVQANSAGQREEYLLCFHEFGVFVDSYGRRSRTDDLKWSRLPLAFAYREPYLFVTHFNSLEVIEIQARSSLGSPARAYLEIPNPRYLGPAISSGAIYLASSYQDKLRVICCKGNLVKESGTEQHRVPSTSRSPNKRGPPTYNEHITKRVASSPAPPEGPSHPREPSTPHRYRDREGRTELRRDKSPGRPLEREKSPGRMLSTRRERSPGRLFEDSSRGRLPAGAVRTPLSQVNKVWDQSSV

Target ORF information:

RefSeq Version	XM_006530134.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus citron (Cit), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006530134.4

ORF Insert Sequence:

ATGTTGAAGT TCAAGTATGG TGTGCGGAAC CCGCCGGAGG CCAGTGCCTC CGAGCCCATT 
GCCAGTCGGG CCTCCAGGCT AAATCTCTTC TTCCAGGGGA AACCGCCCCT CATGACTCAA 
CAGCAGATGT CTGCTCTTTC CCGGGAAGGG ATGCTAGACG CCCTCTTCGC TCTCTTTGAA 
GAGTGCAGCC AACCCGCCCT GATGAAGATG AAGCACGTGA GCAGCTTTGT CCAGAAGTAT 
TCCGACACCA TAGCCGAGTT GCGGGAGCTG CAGCCGTCGG CGAGAGACTT CGAAGTTCGA 
AGCCTTGTGG GCTGTGGTCA CTTCGCTGAA GTGCAGGTGG TTAGAGAGAA GGCGACCGGG 
GACGTCTATG CCATGAAAAT CATGAAGAAG AAGGCTTTGC TGGCCCAGGA ACAGGTTTCA 
TTTTTCGAGG AGGAGAGGAA CATATTATCT CGGAGCACGA GTCCTTGGAT CCCCCAGTTA 
CAGTACGCCT TTCAGGACAA AAATAACCTT TACCTGGTCA TGGAATATCA GCCTGGAGGG 
GATTTGCTGT CGCTTCTGAA CAGATACGAG GACCAATTAG ATGAGAGCAT GATCCAGTTT 
TACCTTGCTG AGCTGATTTT GGCTGTCCAC AGCGTGCACC AGATGGGATA TGTGCATCGA 
GACATCAAGC CCGAGAACAT CCTCATCGAC CGGACGGGAC ACATCAAGCT GGTGGATTTT 
GGATCAGCCG CTAAGATGAA TTCAAATAAG GTGGATGCCA AACTCCCCAT TGGGACCCCG 
GATTACATGG CTCCGGAAGT GTTGACCGTG ATGAACGAGG ACCGAAGGGG CACATACGGC 
TTGGACTGTG ACTGGTGGTC TGTCGGAGTT GTTGCTTATG AGATGGTTTA TGGGAAGACC 
CCATTCACAG AGGGAACCTC CGCCCGGACC TTCAACAACA TCATGAACTT CCAGCGGTTT 
TTGAAGTTCC CAGATGACCC CAAAGTTAGC AGTGAGCTCC TTGATCTGCT TCAGAGTCTG 
CTGTGTGTCC AGAAAGAGAG ACTGAAGTTC GAGGGTCTCT GCTGCCACCC TTTCTTTGCC 
AGAACGGACT GGAACAACAT CCGTAACTCT CCTCCCCCCT TCGTCCCCAC CCTCAAGTCT 
GACGATGACA CCTCCAATTT TGATGAACCA GAGAAGAATT CGTGGGTTTC ATCCTCTGTG 
TGCCAGCTGA GCCCCTCGGG CTTCTCAGGC GAAGAGCTGC CGTTTGTGGG ATTTTCGTAC 
AGCAAGGCAC TGGGGTATCT TGGTAGATCT GAGTCTGTCG TGTCGAGTCT GGACTCCCCT 
GCCAAGGTTA GCTCCATGGA AAAGAAACTT CTCATCAAAA GCAAAGAGCT CCAAGACTCC 
CAGGACAAGT GTCACAAGAT GGAGCAGGAA ATGACCCGGT TACATCGCAG AGTGTCAGAG 
GTGGAGGCTG TGCTTAGTCA GAAGGAGGTG GAGCTGAAGG CCTCTGAGAC TCAGAGATCC 
CTCCTGGAGC AGGACCTTGC TACCTACATC ACAGAATGCA GTAGCTTAAA GCGAAGTTTG 
GAGCAAGCGC GGATGGAGGT GTCCCAGGAG GATGACAAAG CTCTGCAGCT TCTCCACGAC 
ATCCGAGAGC AGAGCCGGAA GCTCCAGGAG ATCAAGGAGC AGGAGTACCA GGCTCAGGTG 
GAGGAGATGA GGCTGATGAT GAATCAGCTG GAAGAAGACC TGGTGTCAGC CCGCAGACGC 
AGCGATCTCT ACGAGTCTGA GCTGAGGGAG TCTCGGCTTG CCGCCGAGGA ATTCAAGCGG 
AAGGCAAACG AATGTCAGCA CAAACTGATG AAGGTAGTCA GCCACCCTCC AAGAGGGGAC 
TCCGGGGGCA CGGCCCTGGA TGATCTTCAC AAGATGCAAG GCCACGCTGG CTTAACGAGT 
GCTAAGGACC AAGGGAAGCC TGAAGTGGGA GAATATTCCA AACTGGAGAA GATCAATGCT 
GAGCAGCAGC TGAAGATCCA GGAGCTCCAG GAGAAGCTGG AGAAGGCTGT AAAAGCCAGC 
ACAGAGGCCA CCGAGCTCCT GCAGAATATC CGCCAGGCAA AGGAGCGAGC AGAGCGGGAG 
CTAGAGAAGC TACACAACCG GGAAGACTCC TCCGAGGGCA TCAAAAAGAA GCTGGTGGAA 
GCCGAGGAAC GCCGCCACTC CCTGGAGAAC AAGGTAAAGA GACTAGAGAC CATGGAGCGT 
AGAGAGAACA GACTGAAGGA TGACATCCAG ACAAAGTCCG AACAGATCCA GCAGATGGCT 
GATAAAATTC TGGAGCTGGA GGAGAAACAT CGGGAGGCTC AGGTCTCAGC TCAACATCTA 
GAAGTACACT TGAAACAGAA AGAACAGCAC TACGAGGAAA AGATCAAGGT ATTGGACAAT 
CAGATAAAGA AGGACCTGGC CGACAAGGAG AGCCTGGAGA ACATGATGCA GAGACACGAG 
GAGGAGGCCC ACGAGAAGGG CAAGATCCTC AGCGAGCAGA AGGCGATGAT CAACGCGATG 
GATTCCAAGA TCCGATCCCT GGAGCAGAGG ATCGTGGAGC TGTCGGAAGC CAACAAGCTT 
GCGGCAAACA GCAGTCTCTT CACCCAGAGG AACATGAAGG CCCAGGAAGA GATGATCTCA 
GAACTCAGGC AGCAGAAATT TTACCTGGAG ACGCAGGCCG GAAAGCTGGA GGCCCAGAAC 
CGAAAGCTGG AAGAGCAACT GGAGAAGATC AGCCACCAAG ATCACAGTGA CAAGAGTCGG 
CTGCTGGAGC TGGAAACAAG GCTGAGGGAG GTCAGCCTGG AGCACGAGGA GCAGAAGCTG 
GAGCTGAAGC GGCAGCTCAC GGAGCTGCAG CTGTCCCTGC AGGAGCGCGA GTCCCAGCTG 
ACGGCCCTGC AGGCCGCCCG GGCAGCGCTG GAGAGCCAGC TCCGCCAGGC GAAGACAGAG 
CTGGAGGAGA CAACCGCGGA AGCGGAGGAG GAGATCCAGG CGCTCACGGC ACATCGAGAT 
GAGATCCAGC GCAAATTCGA TGCCCTTCGC AACAGCTGCA CGGTCATCAC CGACCTGGAG 
GAGCAGCTGA ACCAGCTCAC CGAGGACAAC GCCGAGCTCA ACAACCAAAA CTTCTACCTG 
TCCAAACAAC TCGATGAGGC TTCCGGGGCC AATGACGAGA TTGTGCAGCT GCGGAGTGAG 
GTGGACCATC TCCGCCGTGA GATCACGGAG CGGGAGATGC AGCTCACCAG CCAGAAGCAA 
GCACAACTCA GTGCTCCTGA TTTGCAGACG ATGGAGGCTC TGAAGACGAC ATGCACCATG 
CTGGAGGAGC AGGTCCTGGA CCTGGAGGCC CTGAACGACG AGCTGCTGGA GAAGGAGCGC 
CAGTGGGAGG CCTGGCGGAG CGTCCTCGGC GACGAGAAGT CCCAGTTCGA GTGTCGAGTT 
CGAGAGCTAC AGAGGATGCT GGACACCGAG AAGCAGAGCA GGGCTAGGGC CGATCAGCGG 
ATCACCGAGT CGCGCCAGGT GGTGGAGTTG GCGGTGAAGG AACACAAGGC CGAGATTCTT 
GCTCTGCAGC AGGCTCTCAA GGAGCAGAAG CTCAAAGCCG AGAGCCTGTC GGACAAGCTC 
AACGACCTGG AGAAGAAGCA CGCCATGCTG GAGATGAACG CCCGGAGCTT ACAGCAGAAA 
CTAGAGACAG AGCGGGAGCT CAAACAGAGG CTTCTGGAGG AGCAAGCCAA ATTGCAGCAG 
CAGATGGACC TGCAGAAGAA CCACATCTTC AGACTGACGC AAGGGCTGCA GGAGGCGCTG 
GACCGGGCGG ATCTGCTGAA GACAGAAAGG AGCGACCTGG AGTACCAGCT GGAAAACATT 
CAGGTTCTCT ACTCTCACGA GAAAGTGAAA ATGGAAGGCA CAATCTCTCA GCAAACCAAA 
CTCATTGATT TCCTGCAAGC CAAAATGGAC CAGCCTGCTA AAAAGAAAAA GGGTTTATTT 
AGTCGACGGA AAGAGGACCC TGCTTTGCCC ACACAGGTTC CTCTGCAGTA CAATGAGCTG 
AAGCTAGCCC TGGAAAAGGA GAAAGCCCGA TGCGCGGAGC TGGAGGAGGC CCTTCAGAAG 
ACCCGCATCG AACTCCGCTC TGCGCGGGAG GAAGCTGCCC ACCGCAAAGC CACGGACCAC 
CCGCACCCGT CTACGCCAGC CACTGCGAGG CAGCAGATCG CCATGTCTGC CATTGTGCGG 
TCGCCCGAGC ACCAGCCCAG TGCCATGAGC CTGCTTGCCC CACCATCCAG CCGCAGAAAG 
GAGTCGTCAA CTCCAGAGGA ATTCAGCCGG CGTCTGAAAG AGCGCATGCA CCACAACATC 
CCTCACCGGT TTAATGTGGG CCTGAACATG AGAGCCACCA AGTGCGCCGT GTGTCTGGAT 
ACTGTGCACT TTGGACGCCA GGCATCCAAA TGCCTCGAAT GTCAAGTGAT GTGTCACCCC 
AAATGCTCCA CCTGCTTGCC CGCTACCTGT GGCCTGCCAG CTGAATATGC CACACACTTC 
ACTGAGGCCT TCTGCCGGGA CAAAATGAAC TCCCCGGGGC TCCAGAGCAA GGAGCCTGGC 
AGCAGCTTGC ACCTGGAAGG GTGGATGAAA GTGCCCAGAA ATAACAAACG GGGACAGCAA 
GGCTGGGACA GGAAGTACAT TGTTCTGGAG GGGTCAAAAG TCCTCATCTA TGACAATGAA 
GCCAGAGAAG CTGGACAGAG GCCGGTGGAA GAATTTGAGC TGTGCCTTCC TGACGGGGAC 
GTATCTATTC ATGGTGCCGT TGGTGCTTCA GAACTTGCAA ATACCGCCAA AGCAGATGTC 
CCATACATCC TGAAGATGGA GTCTCACCCA CACACCACCT GCTGGCCTGG GAGAACCCTC 
TACTTGCTAG CACCCAGCTT CCCCGACAAG CAGCGCTGGG TCACCGCCTT AGAATCTGTC 
GTCGCAGGTG GGAGAGTTTC TAGGGAAAAG GCCGAAGCCG ATGCTGCACG TGACCATACT 
TCGAGTGAGC ATCAGCCAGT GTGGGTTGAG AAATTACTTG GAAACTCTCT GCTGAAACTG 
GAGGGCGATG ACCGGCTTGA CATGAACTGC ACCCTGCCCT TCAGTGACCA GGTAGTGCTG 
GTGGGCACGG AGGAAGGCCT CTACGCCCTG AATGTCTTGA AAAACTCCTT AACCCACATC 
CCAGGGATTG GCGCAGTCTT CCAAATTTAC ATCATCAAGG ACCTGGAGAA GCTGCTCATG 
ATAGCAGGGG AAGAGCGGGC TCTGTGCCTG GTGGACGTGA AGAAGGTGAA GCAGTCCCTG 
GCGCAGTCAC ACCTGCCTGC CCAGCCCGAC GTCTCCCCCA ACATATTCGA AGCCGTCAAA 
GGCTGCCACT TGTTCGCTGC TGGCAAGATC GAGAACAGCC TGTGCATCTG CGCCGCTATG 
CCAAGCAAAG TCGTCATCCT CCGCTACAAT GACAACCTCA GCAAGTACTG CATCCGCAAG 
GAGATCGAGA CCTCAGAGCC CTGCAGCTGT ATCCACTTCA CCAACTACAG CATCCTCATT 
GGGACCAACA AATTCTATGA GATCGACATG AAGCAGTACA CGCTTGACGA GTTCCTGGAC 
AAGAACGACC ATTCCTTGGC ACCTGCTGTG TTCGCCTCCT CGTCCAACAG CTTCCCTGTC 
TCCATTGTGC AGGCGAACAG CGCCGGGCAG CGAGAAGAAT ACCTGCTGTG CTTCCACGAA 
TTTGGGGTGT TCGTGGATTC TTACGGAAGA CGTAGCCGCA CAGATGATCT CAAGTGGAGT 
CGCTTACCTC TGGCCTTCGC CTACAGAGAA CCTTATCTGT TTGTGACTCA CTTCAACTCC 
CTGGAAGTCA TTGAGATCCA GGCCAGATCC TCACTGGGGA GCCCTGCCCG AGCATATCTG 
GAAATTCCAA ACCCTCGCTA CCTGGGCCCC GCGATTTCCT CCGGAGCGAT TTACCTGGCC 
TCCTCATACC AGGACAAGTT AAGGGTCATA TGCTGCAAAG GAAACCTCGT GAAGGAGTCA 
GGCACTGAGC AGCACCGGGT GCCCTCCACC TCCCGCAGCC CCAACAAGCG AGGCCCACCA 
ACATACAACG AGCACATCAC CAAACGCGTG GCCTCCAGCC CGGCGCCACC GGAAGGCCCC 
AGCCACCCCC GAGAGCCAAG CACACCGCAC CGCTACCGAG ACAGAGAGGG TCGGACAGAG 
CTGCGCAGGG ACAAGTCTCC AGGCCGCCCT CTGGAGCGGG AGAAGTCCCC AGGCCGAATG 
CTCAGCACTA GGAGAGAGCG GTCCCCAGGG AGACTGTTTG AAGACAGCAG CAGGGGCCGG 
CTGCCTGCAG GAGCAGTGAG GACCCCACTG TCCCAGGTTA ACAAGGTCTG GGACCAGTCT 
TCAGTATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cit ( XM_006530135.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006530198.1 Mus musculus citron (Cit), transcript variant X6, mRNA.

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CloneID	OMu35776
Clone ID Related Accession (Same CDS sequence)	XM_006530135.4 , XM_006530135.3
Accession Version	XM_006530135.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6345bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	citron Rho-interacting kinase isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000071.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006530135.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTGAAGT TCAAGTATGG TGTGCGGAAC CCGCCGGAGG CCAGTGCCTC CGAGCCCATT 
GCCAGTCGGG CCTCCAGGCT AAATCTCTTC TTCCAGGGGA AACCGCCCCT CATGACTCAA 
CAGCAGATGT CTGCTCTTTC CCGGGAAGGG ATGCTAGACG CCCTCTTCGC TCTCTTTGAA 
GAGTGCAGCC AACCCGCCCT GATGAAGATG AAGCACGTGA GCAGCTTTGT CCAGAAGTAT 
TCCGACACCA TAGCCGAGTT GCGGGAGCTG CAGCCGTCGG CGAGAGACTT CGAAGTTCGA 
AGCCTTGTGG GCTGTGGTCA CTTCGCTGAA GTGCAGGTGG TTAGAGAGAA GGCGACCGGG 
GACGTCTATG CCATGAAAAT CATGAAGAAG AAGGCTTTGC TGGCCCAGGA ACAGGTTTCA 
TTTTTCGAGG AGGAGAGGAA CATATTATCT CGGAGCACGA GTCCTTGGAT CCCCCAGTTA 
CAGTACGCCT TTCAGGACAA AAATAACCTT TACCTGGTCA TGGAATATCA GCCTGGAGGG 
GATTTGCTGT CGCTTCTGAA CAGATACGAG GACCAATTAG ATGAGAGCAT GATCCAGTTT 
TACCTTGCTG AGCTGATTTT GGCTGTCCAC AGCGTGCACC AGATGGGATA TGTGCATCGA 
GACATCAAGC CCGAGAACAT CCTCATCGAC CGGACGGGAC ACATCAAGCT GGTGGATTTT 
GGATCAGCCG CTAAGATGAA TTCAAATAAG GTGGATGCCA AACTCCCCAT TGGGACCCCG 
GATTACATGG CTCCGGAAGT GTTGACCGTG ATGAACGAGG ACCGAAGGGG CACATACGGC 
TTGGACTGTG ACTGGTGGTC TGTCGGAGTT GTTGCTTATG AGATGGTTTA TGGGAAGACC 
CCATTCACAG AGGGAACCTC CGCCCGGACC TTCAACAACA TCATGAACTT CCAGCGGTTT 
TTGAAGTTCC CAGATGACCC CAAAGTTAGC AGTGAGCTCC TTGATCTGCT TCAGAGTCTG 
CTGTGTGTCC AGAAAGAGAG ACTGAAGTTC GAGGGTCTCT GCTGCCACCC TTTCTTTGCC 
AGAACGGACT GGAACAACAT CCGTAACTCT CCTCCCCCCT TCGTCCCCAC CCTCAAGTCT 
GACGATGACA CCTCCAATTT TGATGAACCA GAGAAGAATT CGTGGGTTTC ATCCTCTGTG 
TGCCAGCTGA GCCCCTCGGG CTTCTCAGGC GAAGAGCTGC CGTTTGTGGG ATTTTCGTAC 
AGCAAGGCAC TGGGGTATCT TGGTAGATCT GAGTCTGTCG TGTCGAGTCT GGACTCCCCT 
GCCAAGGTTA GCTCCATGGA AAAGAAACTT CTCATCAAAA GCAAAGAGCT CCAAGACTCC 
CAGGACAAGT GTCACAAGAT GGAGCAGGAA ATGACCCGGT TACATCGCAG AGTGTCAGAG 
GTGGAGGCTG TGCTTAGTCA GAAGGAGGTG GAGCTGAAGG CCTCTGAGAC TCAGAGATCC 
CTCCTGGAGC AGGACCTTGC TACCTACATC ACAGAATGCA GTAGCTTAAA GCGAAGTTTG 
GAGCAAGCGC GGATGGAGGT GTCCCAGGAG GATGACAAAG CTCTGCAGCT TCTCCACGAC 
ATCCGAGAGC AGAGCCGGAA GCTCCAGGAG ATCAAGGAGC AGGAGTACCA GGCTCAGGTG 
GAGGAGATGA GGCTGATGAT GAATCAGCTG GAAGAAGACC TGGTGTCAGC CCGCAGACGC 
AGCGATCTCT ACGAGTCTGA GCTGAGGGAG TCTCGGCTTG CCGCCGAGGA ATTCAAGCGG 
AAGGCAAACG AATGTCAGCA CAAACTGATG AAGGTAGTCA GCCACCCTCC AAGAGGGGAC 
TCCGGGGGCA CGGCCCTGGA TGATCTTCAC AAGATGCAAG GCCACGCTGG CTTAACGAGT 
GCTAAGGACC AAGGGAAGCC TGAAGTGGGA GAATATTCCA AACTGGAGAA GATCAATGCT 
GAGCAGCAGC TGAAGATCCA GGAGCTCCAG GAGAAGCTGG AGAAGGCTGT AAAAGCCAGC 
ACAGAGGCCA CCGAGCTCCT GCAGAATATC CGCCAGGCAA AGGAGCGAGC AGAGCGGGAG 
CTAGAGAAGC TACACAACCG GGAAGACTCC TCCGAGGGCA TCAAAAAGAA GCTGGTGGAA 
GCCGAGGAAC GCCGCCACTC CCTGGAGAAC AAGGTAAAGA GACTAGAGAC CATGGAGCGT 
AGAGAGAACA GACTGAAGGA TGACATCCAG ACAAAGTCCG AACAGATCCA GCAGATGGCT 
GATAAAATTC TGGAGCTGGA GGAGAAACAT CGGGAGGCTC AGGTCTCAGC TCAACATCTA 
GAAGTACACT TGAAACAGAA AGAACAGCAC TACGAGGAAA AGATCAAGGT ATTGGACAAT 
CAGATAAAGA AGGACCTGGC CGACAAGGAG AGCCTGGAGA ACATGATGCA GAGACACGAG 
GAGGAGGCCC ACGAGAAGGG CAAGATCCTC AGCGAGCAGA AGGCGATGAT CAACGCGATG 
GATTCCAAGA TCCGATCCCT GGAGCAGAGG ATCGTGGAGC TGTCGGAAGC CAACAAGCTT 
GCGGCAAACA GCAGTCTCTT CACCCAGAGG AACATGAAGG CCCAGGAAGA GATGATCTCA 
GAACTCAGGC AGCAGAAATT TTACCTGGAG ACGCAGGCCG GAAAGCTGGA GGCCCAGAAC 
CGAAAGCTGG AAGAGCAACT GGAGAAGATC AGCCACCAAG ATCACAGTGA CAAGAGTCGG 
CTGCTGGAGC TGGAAACAAG GCTGAGGGAG GTCAGCCTGG AGCACGAGGA GCAGAAGCTG 
GAGCTGAAGC GGCAGCTCAC GGAGCTGCAG CTGTCCCTGC AGGAGCGCGA GTCCCAGCTG 
ACGGCCCTGC AGGCCGCCCG GGCAGCGCTG GAGAGCCAGC TCCGCCAGGC GAAGACAGAG 
CTGGAGGAGA CAACCGCGGA AGCGGAGGAG GAGATCCAGG CGCTCACGGC ACATCGAGAT 
GAGATCCAGC GCAAATTCGA TGCCCTTCGC AACAGCTGCA CGGTCATCAC CGACCTGGAG 
GAGCAGCTGA ACCAGCTCAC CGAGGACAAC GCCGAGCTCA ACAACCAAAA CTTCTACCTG 
TCCAAACAAC TCGATGAGGC TTCCGGGGCC AATGACGAGA TTGTGCAGCT GCGGAGTGAG 
GTGGACCATC TCCGCCGTGA GATCACGGAG CGGGAGATGC AGCTCACCAG CCAGAAGCAA 
ACGATGGAGG CTCTGAAGAC GACATGCACC ATGCTGGAGG AGCAGGTCCT GGACCTGGAG 
GCCCTGAACG ACGAGCTGCT GGAGAAGGAG CGCCAGTGGG AGGCCTGGCG GAGCGTCCTC 
GGCGACGAGA AGTCCCAGTT CGAGTGTCGA GTTCGAGAGC TACAGAGGAT GCTGGACACC 
GAGAAGCAGA GCAGGGCTAG GGCCGATCAG CGGATCACCG AGTCGCGCCA GGTGGTGGAG 
TTGGCGGTGA AGGAACACAA GGCCGAGATT CTTGCTCTGC AGCAGGCTCT CAAGGAGCAG 
AAGCTCAAAG CCGAGAGCCT GTCGGACAAG CTCAACGACC TGGAGAAGAA GCACGCCATG 
CTGGAGATGA ACGCCCGGAG CTTACAGCAG AAACTAGAGA CAGAGCGGGA GCTCAAACAG 
AGGCTTCTGG AGGAGCAAGC CAAATTGCAG CAGCAGATGG ACCTGCAGAA GAACCACATC 
TTCAGACTGA CGCAAGGGCT GCAGGAGGCG CTGGACCGGG CGGATCTGCT GAAGACAGAA 
AGGAGCGACC TGGAGTACCA GCTGGAAAAC ATTCAGGTTC TCTACTCTCA CGAGAAAGTG 
AAAATGGAAG GCACAATCTC TCAGCAAACC AAACTCATTG ATTTCCTGCA AGCCAAAATG 
GACCAGCCTG CTAAAAAGAA AAAGGGTTTA TTTAGTCGAC GGAAAGAGGA CCCTGCTTTG 
CCCACACAGG TTCCTCTGCA GTACAATGAG CTGAAGCTAG CCCTGGAAAA GGAGAAAGCC 
CGATGCGCGG AGCTGGAGGA GGCCCTTCAG AAGACCCGCA TCGAACTCCG CTCTGCGCGG 
GAGGAAGCTG CCCACCGCAA AGCCACGGAC CACCCGCACC CGTCTACGCC AGCCACTGCG 
AGGCAGCAGA TCGCCATGTC TGCCATTGTG CGGTCGCCCG AGCACCAGCC CAGTGCCATG 
AGCCTGCTTG CCCCACCATC CAGCCGCAGA AAGGAGTCGT CAACTCCAGA GGAATTCAGC 
CGGCGTCTGA AAGAGCGCAT GCACCACAAC ATCCCTCACC GGTTTAATGT GGGCCTGAAC 
ATGAGAGCCA CCAAGTGCGC CGTGTGTCTG GATACTGTGC ACTTTGGACG CCAGGCATCC 
AAATGCCTCG AATGTCAAGT GATGTGTCAC CCCAAATGCT CCACCTGCTT GCCCGCTACC 
TGTGGCCTGC CAGCTGAATA TGCCACACAC TTCACTGAGG CCTTCTGCCG GGACAAAATG 
AACTCCCCGG GGCTCCAGAG CAAGGAGCCT GGCAGCAGCT TGCACCTGGA AGGGTGGATG 
AAAGTGCCCA GAAATAACAA ACGGGGACAG CAAGGCTGGG ACAGGAAGTA CATTGTTCTG 
GAGGGGTCAA AAGTCCTCAT CTATGACAAT GAAGCCAGAG AAGCTGGACA GAGGCCGGTG 
GAAGAATTTG AGCTGTGCCT TCCTGACGGG GACGTATCTA TTCATGGTGC CGTTGGTGCT 
TCAGAACTTG CAAATACCGC CAAAGCAGAT GTCCCATACA TCCTGAAGAT GGAGTCTCAC 
CCACACACCA CCTGCTGGCC TGGGAGAACC CTCTACTTGC TAGCACCCAG CTTCCCCGAC 
AAGCAGCGCT GGGTCACCGC CTTAGAATCT GTCGTCGCAG GTGGGAGAGT TTCTAGGGAA 
AAGGCCGAAG CCGATGCTGC ACGTGACCAT ACTTCGAGTG AGCATCAGCC AGTGTGGGTT 
GAGAAATTAC TTGGAAACTC TCTGCTGAAA CTGGAGGGCG ATGACCGGCT TGACATGAAC 
TGCACCCTGC CCTTCAGTGA CCAGGTAGTG CTGGTGGGCA CGGAGGAAGG CCTCTACGCC 
CTGAATGTCT TGAAAAACTC CTTAACCCAC ATCCCAGGGA TTGGCGCAGT CTTCCAAATT 
TACATCATCA AGGACCTGGA GAAGCTGCTC ATGATAGCAG GGGAAGAGCG GGCTCTGTGC 
CTGGTGGACG TGAAGAAGGT GAAGCAGTCC CTGGCGCAGT CACACCTGCC TGCCCAGCCC 
GACGTCTCCC CCAACATATT CGAAGCCGTC AAAGGCTGCC ACTTGTTCGC TGCTGGCAAG 
ATCGAGAACA GCCTGTGCAT CTGCGCCGCT ATGCCAAGCA AAGTCGTCAT CCTCCGCTAC 
AATGACAACC TCAGCAAGTA CTGCATCCGC AAGGAGATCG AGACCTCAGA GCCCTGCAGC 
TGTATCCACT TCACCAACTA CAGCATCCTC ATTGGGACCA ACAAATTCTA TGAGATCGAC 
ATGAAGCAGT ACACGCTTGA CGAGTTCCTG GACAAGAACG ACCATTCCTT GGCACCTGCT 
GTGTTCGCCT CCTCGTCCAA CAGCTTCCCT GTCTCCATTG TGCAGGCGAA CAGCGCCGGG 
CAGCGAGAAG AATACCTGCT GTGCTTCCAC GAATTTGGGG TGTTCGTGGA TTCTTACGGA 
AGACGTAGCC GCACAGATGA TCTCAAGTGG AGTCGCTTAC CTCTGGCCTT CGCCTACAGA 
GAACCTTATC TGTTTGTGAC TCACTTCAAC TCCCTGGAAG TCATTGAGAT CCAGGCCAGA 
TCCTCACTGG GGAGCCCTGC CCGAGCATAT CTGGAAATTC CAAACCCTCG CTACCTGGGC 
CCCGCGATTT CCTCCGGAGC GATTTACCTG GCCTCCTCAT ACCAGGACAA GTTAAGGGTC 
ATATGCTGCA AAGGAAACCT CGTGAAGGAG TCAGGCACTG AGCAGCACCG GGTGCCCTCC 
ACCTCCCGCA GCAGCCCCAA CAAGCGAGGC CCACCAACAT ACAACGAGCA CATCACCAAA 
CGCGTGGCCT CCAGCCCGGC GCCACCGGAA GGCCCCAGCC ACCCCCGAGA GCCAAGCACA 
CCGCACCGCT ACCGAGACAG AGAGGGTCGG ACAGAGCTGC GCAGGGACAA GTCTCCAGGC 
CGCCCTCTGG AGCGGGAGAA GTCCCCAGGC CGAATGCTCA GCACTAGGAG AGAGCGGTCC 
CCAGGGAGAC TGTTTGAAGA CAGCAGCAGG GGCCGGCTGC CTGCAGGAGC AGTGAGGACC 
CCACTGTCCC AGGTTAACAA GGTCTGGGAC CAGTCTTCAG TATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006530198.1
CDS	158..6502
Translation	MLKFKYGVRNPPEASASEPIASRASRLNLFFQGKPPLMTQQQMSALSREGMLDALFALFEECSQPALMKMKHVSSFVQKYSDTIAELRELQPSARDFEVRSLVGCGHFAEVQVVREKATGDVYAMKIMKKKALLAQEQVSFFEEERNILSRSTSPWIPQLQYAFQDKNNLYLVMEYQPGGDLLSLLNRYEDQLDESMIQFYLAELILAVHSVHQMGYVHRDIKPENILIDRTGHIKLVDFGSAAKMNSNKVDAKLPIGTPDYMAPEVLTVMNEDRRGTYGLDCDWWSVGVVAYEMVYGKTPFTEGTSARTFNNIMNFQRFLKFPDDPKVSSELLDLLQSLLCVQKERLKFEGLCCHPFFARTDWNNIRNSPPPFVPTLKSDDDTSNFDEPEKNSWVSSSVCQLSPSGFSGEELPFVGFSYSKALGYLGRSESVVSSLDSPAKVSSMEKKLLIKSKELQDSQDKCHKMEQEMTRLHRRVSEVEAVLSQKEVELKASETQRSLLEQDLATYITECSSLKRSLEQARMEVSQEDDKALQLLHDIREQSRKLQEIKEQEYQAQVEEMRLMMNQLEEDLVSARRRSDLYESELRESRLAAEEFKRKANECQHKLMKVVSHPPRGDSGGTALDDLHKMQGHAGLTSAKDQGKPEVGEYSKLEKINAEQQLKIQELQEKLEKAVKASTEATELLQNIRQAKERAERELEKLHNREDSSEGIKKKLVEAEERRHSLENKVKRLETMERRENRLKDDIQTKSEQIQQMADKILELEEKHREAQVSAQHLEVHLKQKEQHYEEKIKVLDNQIKKDLADKESLENMMQRHEEEAHEKGKILSEQKAMINAMDSKIRSLEQRIVELSEANKLAANSSLFTQRNMKAQEEMISELRQQKFYLETQAGKLEAQNRKLEEQLEKISHQDHSDKSRLLELETRLREVSLEHEEQKLELKRQLTELQLSLQERESQLTALQAARAALESQLRQAKTELEETTAEAEEEIQALTAHRDEIQRKFDALRNSCTVITDLEEQLNQLTEDNAELNNQNFYLSKQLDEASGANDEIVQLRSEVDHLRREITEREMQLTSQKQTMEALKTTCTMLEEQVLDLEALNDELLEKERQWEAWRSVLGDEKSQFECRVRELQRMLDTEKQSRARADQRITESRQVVELAVKEHKAEILALQQALKEQKLKAESLSDKLNDLEKKHAMLEMNARSLQQKLETERELKQRLLEEQAKLQQQMDLQKNHIFRLTQGLQEALDRADLLKTERSDLEYQLENIQVLYSHEKVKMEGTISQQTKLIDFLQAKMDQPAKKKKGLFSRRKEDPALPTQVPLQYNELKLALEKEKARCAELEEALQKTRIELRSAREEAAHRKATDHPHPSTPATARQQIAMSAIVRSPEHQPSAMSLLAPPSSRRKESSTPEEFSRRLKERMHHNIPHRFNVGLNMRATKCAVCLDTVHFGRQASKCLECQVMCHPKCSTCLPATCGLPAEYATHFTEAFCRDKMNSPGLQSKEPGSSLHLEGWMKVPRNNKRGQQGWDRKYIVLEGSKVLIYDNEAREAGQRPVEEFELCLPDGDVSIHGAVGASELANTAKADVPYILKMESHPHTTCWPGRTLYLLAPSFPDKQRWVTALESVVAGGRVSREKAEADAARDHTSSEHQPVWVEKLLGNSLLKLEGDDRLDMNCTLPFSDQVVLVGTEEGLYALNVLKNSLTHIPGIGAVFQIYIIKDLEKLLMIAGEERALCLVDVKKVKQSLAQSHLPAQPDVSPNIFEAVKGCHLFAAGKIENSLCICAAMPSKVVILRYNDNLSKYCIRKEIETSEPCSCIHFTNYSILIGTNKFYEIDMKQYTLDEFLDKNDHSLAPAVFASSSNSFPVSIVQANSAGQREEYLLCFHEFGVFVDSYGRRSRTDDLKWSRLPLAFAYREPYLFVTHFNSLEVIEIQARSSLGSPARAYLEIPNPRYLGPAISSGAIYLASSYQDKLRVICCKGNLVKESGTEQHRVPSTSRSSPNKRGPPTYNEHITKRVASSPAPPEGPSHPREPSTPHRYRDREGRTELRRDKSPGRPLEREKSPGRMLSTRRERSPGRLFEDSSRGRLPAGAVRTPLSQVNKVWDQSSV

Target ORF information:

RefSeq Version	XM_006530135.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus citron (Cit), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006530135.4

ORF Insert Sequence:

ATGTTGAAGT TCAAGTATGG TGTGCGGAAC CCGCCGGAGG CCAGTGCCTC CGAGCCCATT 
GCCAGTCGGG CCTCCAGGCT AAATCTCTTC TTCCAGGGGA AACCGCCCCT CATGACTCAA 
CAGCAGATGT CTGCTCTTTC CCGGGAAGGG ATGCTAGACG CCCTCTTCGC TCTCTTTGAA 
GAGTGCAGCC AACCCGCCCT GATGAAGATG AAGCACGTGA GCAGCTTTGT CCAGAAGTAT 
TCCGACACCA TAGCCGAGTT GCGGGAGCTG CAGCCGTCGG CGAGAGACTT CGAAGTTCGA 
AGCCTTGTGG GCTGTGGTCA CTTCGCTGAA GTGCAGGTGG TTAGAGAGAA GGCGACCGGG 
GACGTCTATG CCATGAAAAT CATGAAGAAG AAGGCTTTGC TGGCCCAGGA ACAGGTTTCA 
TTTTTCGAGG AGGAGAGGAA CATATTATCT CGGAGCACGA GTCCTTGGAT CCCCCAGTTA 
CAGTACGCCT TTCAGGACAA AAATAACCTT TACCTGGTCA TGGAATATCA GCCTGGAGGG 
GATTTGCTGT CGCTTCTGAA CAGATACGAG GACCAATTAG ATGAGAGCAT GATCCAGTTT 
TACCTTGCTG AGCTGATTTT GGCTGTCCAC AGCGTGCACC AGATGGGATA TGTGCATCGA 
GACATCAAGC CCGAGAACAT CCTCATCGAC CGGACGGGAC ACATCAAGCT GGTGGATTTT 
GGATCAGCCG CTAAGATGAA TTCAAATAAG GTGGATGCCA AACTCCCCAT TGGGACCCCG 
GATTACATGG CTCCGGAAGT GTTGACCGTG ATGAACGAGG ACCGAAGGGG CACATACGGC 
TTGGACTGTG ACTGGTGGTC TGTCGGAGTT GTTGCTTATG AGATGGTTTA TGGGAAGACC 
CCATTCACAG AGGGAACCTC CGCCCGGACC TTCAACAACA TCATGAACTT CCAGCGGTTT 
TTGAAGTTCC CAGATGACCC CAAAGTTAGC AGTGAGCTCC TTGATCTGCT TCAGAGTCTG 
CTGTGTGTCC AGAAAGAGAG ACTGAAGTTC GAGGGTCTCT GCTGCCACCC TTTCTTTGCC 
AGAACGGACT GGAACAACAT CCGTAACTCT CCTCCCCCCT TCGTCCCCAC CCTCAAGTCT 
GACGATGACA CCTCCAATTT TGATGAACCA GAGAAGAATT CGTGGGTTTC ATCCTCTGTG 
TGCCAGCTGA GCCCCTCGGG CTTCTCAGGC GAAGAGCTGC CGTTTGTGGG ATTTTCGTAC 
AGCAAGGCAC TGGGGTATCT TGGTAGATCT GAGTCTGTCG TGTCGAGTCT GGACTCCCCT 
GCCAAGGTTA GCTCCATGGA AAAGAAACTT CTCATCAAAA GCAAAGAGCT CCAAGACTCC 
CAGGACAAGT GTCACAAGAT GGAGCAGGAA ATGACCCGGT TACATCGCAG AGTGTCAGAG 
GTGGAGGCTG TGCTTAGTCA GAAGGAGGTG GAGCTGAAGG CCTCTGAGAC TCAGAGATCC 
CTCCTGGAGC AGGACCTTGC TACCTACATC ACAGAATGCA GTAGCTTAAA GCGAAGTTTG 
GAGCAAGCGC GGATGGAGGT GTCCCAGGAG GATGACAAAG CTCTGCAGCT TCTCCACGAC 
ATCCGAGAGC AGAGCCGGAA GCTCCAGGAG ATCAAGGAGC AGGAGTACCA GGCTCAGGTG 
GAGGAGATGA GGCTGATGAT GAATCAGCTG GAAGAAGACC TGGTGTCAGC CCGCAGACGC 
AGCGATCTCT ACGAGTCTGA GCTGAGGGAG TCTCGGCTTG CCGCCGAGGA ATTCAAGCGG 
AAGGCAAACG AATGTCAGCA CAAACTGATG AAGGTAGTCA GCCACCCTCC AAGAGGGGAC 
TCCGGGGGCA CGGCCCTGGA TGATCTTCAC AAGATGCAAG GCCACGCTGG CTTAACGAGT 
GCTAAGGACC AAGGGAAGCC TGAAGTGGGA GAATATTCCA AACTGGAGAA GATCAATGCT 
GAGCAGCAGC TGAAGATCCA GGAGCTCCAG GAGAAGCTGG AGAAGGCTGT AAAAGCCAGC 
ACAGAGGCCA CCGAGCTCCT GCAGAATATC CGCCAGGCAA AGGAGCGAGC AGAGCGGGAG 
CTAGAGAAGC TACACAACCG GGAAGACTCC TCCGAGGGCA TCAAAAAGAA GCTGGTGGAA 
GCCGAGGAAC GCCGCCACTC CCTGGAGAAC AAGGTAAAGA GACTAGAGAC CATGGAGCGT 
AGAGAGAACA GACTGAAGGA TGACATCCAG ACAAAGTCCG AACAGATCCA GCAGATGGCT 
GATAAAATTC TGGAGCTGGA GGAGAAACAT CGGGAGGCTC AGGTCTCAGC TCAACATCTA 
GAAGTACACT TGAAACAGAA AGAACAGCAC TACGAGGAAA AGATCAAGGT ATTGGACAAT 
CAGATAAAGA AGGACCTGGC CGACAAGGAG AGCCTGGAGA ACATGATGCA GAGACACGAG 
GAGGAGGCCC ACGAGAAGGG CAAGATCCTC AGCGAGCAGA AGGCGATGAT CAACGCGATG 
GATTCCAAGA TCCGATCCCT GGAGCAGAGG ATCGTGGAGC TGTCGGAAGC CAACAAGCTT 
GCGGCAAACA GCAGTCTCTT CACCCAGAGG AACATGAAGG CCCAGGAAGA GATGATCTCA 
GAACTCAGGC AGCAGAAATT TTACCTGGAG ACGCAGGCCG GAAAGCTGGA GGCCCAGAAC 
CGAAAGCTGG AAGAGCAACT GGAGAAGATC AGCCACCAAG ATCACAGTGA CAAGAGTCGG 
CTGCTGGAGC TGGAAACAAG GCTGAGGGAG GTCAGCCTGG AGCACGAGGA GCAGAAGCTG 
GAGCTGAAGC GGCAGCTCAC GGAGCTGCAG CTGTCCCTGC AGGAGCGCGA GTCCCAGCTG 
ACGGCCCTGC AGGCCGCCCG GGCAGCGCTG GAGAGCCAGC TCCGCCAGGC GAAGACAGAG 
CTGGAGGAGA CAACCGCGGA AGCGGAGGAG GAGATCCAGG CGCTCACGGC ACATCGAGAT 
GAGATCCAGC GCAAATTCGA TGCCCTTCGC AACAGCTGCA CGGTCATCAC CGACCTGGAG 
GAGCAGCTGA ACCAGCTCAC CGAGGACAAC GCCGAGCTCA ACAACCAAAA CTTCTACCTG 
TCCAAACAAC TCGATGAGGC TTCCGGGGCC AATGACGAGA TTGTGCAGCT GCGGAGTGAG 
GTGGACCATC TCCGCCGTGA GATCACGGAG CGGGAGATGC AGCTCACCAG CCAGAAGCAA 
ACGATGGAGG CTCTGAAGAC GACATGCACC ATGCTGGAGG AGCAGGTCCT GGACCTGGAG 
GCCCTGAACG ACGAGCTGCT GGAGAAGGAG CGCCAGTGGG AGGCCTGGCG GAGCGTCCTC 
GGCGACGAGA AGTCCCAGTT CGAGTGTCGA GTTCGAGAGC TACAGAGGAT GCTGGACACC 
GAGAAGCAGA GCAGGGCTAG GGCCGATCAG CGGATCACCG AGTCGCGCCA GGTGGTGGAG 
TTGGCGGTGA AGGAACACAA GGCCGAGATT CTTGCTCTGC AGCAGGCTCT CAAGGAGCAG 
AAGCTCAAAG CCGAGAGCCT GTCGGACAAG CTCAACGACC TGGAGAAGAA GCACGCCATG 
CTGGAGATGA ACGCCCGGAG CTTACAGCAG AAACTAGAGA CAGAGCGGGA GCTCAAACAG 
AGGCTTCTGG AGGAGCAAGC CAAATTGCAG CAGCAGATGG ACCTGCAGAA GAACCACATC 
TTCAGACTGA CGCAAGGGCT GCAGGAGGCG CTGGACCGGG CGGATCTGCT GAAGACAGAA 
AGGAGCGACC TGGAGTACCA GCTGGAAAAC ATTCAGGTTC TCTACTCTCA CGAGAAAGTG 
AAAATGGAAG GCACAATCTC TCAGCAAACC AAACTCATTG ATTTCCTGCA AGCCAAAATG 
GACCAGCCTG CTAAAAAGAA AAAGGGTTTA TTTAGTCGAC GGAAAGAGGA CCCTGCTTTG 
CCCACACAGG TTCCTCTGCA GTACAATGAG CTGAAGCTAG CCCTGGAAAA GGAGAAAGCC 
CGATGCGCGG AGCTGGAGGA GGCCCTTCAG AAGACCCGCA TCGAACTCCG CTCTGCGCGG 
GAGGAAGCTG CCCACCGCAA AGCCACGGAC CACCCGCACC CGTCTACGCC AGCCACTGCG 
AGGCAGCAGA TCGCCATGTC TGCCATTGTG CGGTCGCCCG AGCACCAGCC CAGTGCCATG 
AGCCTGCTTG CCCCACCATC CAGCCGCAGA AAGGAGTCGT CAACTCCAGA GGAATTCAGC 
CGGCGTCTGA AAGAGCGCAT GCACCACAAC ATCCCTCACC GGTTTAATGT GGGCCTGAAC 
ATGAGAGCCA CCAAGTGCGC CGTGTGTCTG GATACTGTGC ACTTTGGACG CCAGGCATCC 
AAATGCCTCG AATGTCAAGT GATGTGTCAC CCCAAATGCT CCACCTGCTT GCCCGCTACC 
TGTGGCCTGC CAGCTGAATA TGCCACACAC TTCACTGAGG CCTTCTGCCG GGACAAAATG 
AACTCCCCGG GGCTCCAGAG CAAGGAGCCT GGCAGCAGCT TGCACCTGGA AGGGTGGATG 
AAAGTGCCCA GAAATAACAA ACGGGGACAG CAAGGCTGGG ACAGGAAGTA CATTGTTCTG 
GAGGGGTCAA AAGTCCTCAT CTATGACAAT GAAGCCAGAG AAGCTGGACA GAGGCCGGTG 
GAAGAATTTG AGCTGTGCCT TCCTGACGGG GACGTATCTA TTCATGGTGC CGTTGGTGCT 
TCAGAACTTG CAAATACCGC CAAAGCAGAT GTCCCATACA TCCTGAAGAT GGAGTCTCAC 
CCACACACCA CCTGCTGGCC TGGGAGAACC CTCTACTTGC TAGCACCCAG CTTCCCCGAC 
AAGCAGCGCT GGGTCACCGC CTTAGAATCT GTCGTCGCAG GTGGGAGAGT TTCTAGGGAA 
AAGGCCGAAG CCGATGCTGC ACGTGACCAT ACTTCGAGTG AGCATCAGCC AGTGTGGGTT 
GAGAAATTAC TTGGAAACTC TCTGCTGAAA CTGGAGGGCG ATGACCGGCT TGACATGAAC 
TGCACCCTGC CCTTCAGTGA CCAGGTAGTG CTGGTGGGCA CGGAGGAAGG CCTCTACGCC 
CTGAATGTCT TGAAAAACTC CTTAACCCAC ATCCCAGGGA TTGGCGCAGT CTTCCAAATT 
TACATCATCA AGGACCTGGA GAAGCTGCTC ATGATAGCAG GGGAAGAGCG GGCTCTGTGC 
CTGGTGGACG TGAAGAAGGT GAAGCAGTCC CTGGCGCAGT CACACCTGCC TGCCCAGCCC 
GACGTCTCCC CCAACATATT CGAAGCCGTC AAAGGCTGCC ACTTGTTCGC TGCTGGCAAG 
ATCGAGAACA GCCTGTGCAT CTGCGCCGCT ATGCCAAGCA AAGTCGTCAT CCTCCGCTAC 
AATGACAACC TCAGCAAGTA CTGCATCCGC AAGGAGATCG AGACCTCAGA GCCCTGCAGC 
TGTATCCACT TCACCAACTA CAGCATCCTC ATTGGGACCA ACAAATTCTA TGAGATCGAC 
ATGAAGCAGT ACACGCTTGA CGAGTTCCTG GACAAGAACG ACCATTCCTT GGCACCTGCT 
GTGTTCGCCT CCTCGTCCAA CAGCTTCCCT GTCTCCATTG TGCAGGCGAA CAGCGCCGGG 
CAGCGAGAAG AATACCTGCT GTGCTTCCAC GAATTTGGGG TGTTCGTGGA TTCTTACGGA 
AGACGTAGCC GCACAGATGA TCTCAAGTGG AGTCGCTTAC CTCTGGCCTT CGCCTACAGA 
GAACCTTATC TGTTTGTGAC TCACTTCAAC TCCCTGGAAG TCATTGAGAT CCAGGCCAGA 
TCCTCACTGG GGAGCCCTGC CCGAGCATAT CTGGAAATTC CAAACCCTCG CTACCTGGGC 
CCCGCGATTT CCTCCGGAGC GATTTACCTG GCCTCCTCAT ACCAGGACAA GTTAAGGGTC 
ATATGCTGCA AAGGAAACCT CGTGAAGGAG TCAGGCACTG AGCAGCACCG GGTGCCCTCC 
ACCTCCCGCA GCAGCCCCAA CAAGCGAGGC CCACCAACAT ACAACGAGCA CATCACCAAA 
CGCGTGGCCT CCAGCCCGGC GCCACCGGAA GGCCCCAGCC ACCCCCGAGA GCCAAGCACA 
CCGCACCGCT ACCGAGACAG AGAGGGTCGG ACAGAGCTGC GCAGGGACAA GTCTCCAGGC 
CGCCCTCTGG AGCGGGAGAA GTCCCCAGGC CGAATGCTCA GCACTAGGAG AGAGCGGTCC 
CCAGGGAGAC TGTTTGAAGA CAGCAGCAGG GGCCGGCTGC CTGCAGGAGC AGTGAGGACC 
CCACTGTCCC AGGTTAACAA GGTCTGGGAC CAGTCTTCAG TATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cit ( XM_006530136.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006530199.1 Mus musculus citron (Cit), transcript variant X7, mRNA.

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CloneID	OMu35777
Clone ID Related Accession (Same CDS sequence)	XM_006530136.3 , XM_006530136.4
Accession Version	XM_006530136.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6327bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	citron Rho-interacting kinase isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000071.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006530136.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTGAAGT TCAAGTATGG TGTGCGGAAC CCGCCGGAGG CCAGTGCCTC CGAGCCCATT 
GCCAGTCGGG CCTCCAGGCT AAATCTCTTC TTCCAGGGGA AACCGCCCCT CATGACTCAA 
CAGCAGATGT CTGCTCTTTC CCGGGAAGGG ATGCTAGACG CCCTCTTCGC TCTCTTTGAA 
GAGTGCAGCC AACCCGCCCT GATGAAGATG AAGCACGTGA GCAGCTTTGT CCAGAAGTAT 
TCCGACACCA TAGCCGAGTT GCGGGAGCTG CAGCCGTCGG CGAGAGACTT CGAAGTTCGA 
AGCCTTGTGG GCTGTGGTCA CTTCGCTGAA GTGCAGGTGG TTAGAGAGAA GGCGACCGGG 
GACGTCTATG CCATGAAAAT CATGAAGAAG AAGGCTTTGC TGGCCCAGGA ACAGGTTTCA 
TTTTTCGAGG AGGAGAGGAA CATATTATCT CGGAGCACGA GTCCTTGGAT CCCCCAGTTA 
CAGTACGCCT TTCAGGACAA AAATAACCTT TACCTGGTCA TGGAATATCA GCCTGGAGGG 
GATTTGCTGT CGCTTCTGAA CAGATACGAG GACCAATTAG ATGAGAGCAT GATCCAGTTT 
TACCTTGCTG AGCTGATTTT GGCTGTCCAC AGCGTGCACC AGATGGGATA TGTGCATCGA 
GACATCAAGC CCGAGAACAT CCTCATCGAC CGGACGGGAC ACATCAAGCT GGTGGATTTT 
GGATCAGCCG CTAAGATGAA TTCAAATAAG GTGGATGCCA AACTCCCCAT TGGGACCCCG 
GATTACATGG CTCCGGAAGT GTTGACCGTG ATGAACGAGG ACCGAAGGGG CACATACGGC 
TTGGACTGTG ACTGGTGGTC TGTCGGAGTT GTTGCTTATG AGATGGTTTA TGGGAAGACC 
CCATTCACAG AGGGAACCTC CGCCCGGACC TTCAACAACA TCATGAACTT CCAGCGGTTT 
TTGAAGTTCC CAGATGACCC CAAAGTTAGC AGTGAGCTCC TTGATCTGCT TCAGAGTCTG 
CTGTGTGTCC AGAAAGAGAG ACTGAAGTTC GAGGGTCTCT GCTGCCACCC TTTCTTTGCC 
AGAACGGACT GGAACAACAT CCGTAACTCT CCTCCCCCCT TCGTCCCCAC CCTCAAGTCT 
GACGATGACA CCTCCAATTT TGATGAACCA GAGAAGAATT CGTGGGTTTC ATCCTCTGTG 
TGCCAGCTGA GCCCCTCGGG CTTCTCAGGC GAAGAGCTGC CGTTTGTGGG ATTTTCGTAC 
AGCAAGGCAC TGGGGTATCT TGGTAGATCT GAGTCTGTCG TGTCGAGTCT GGACTCCCCT 
GCCAAGGTTA GCTCCATGGA AAAGAAACTT CTCATCAAAA GCAAAGAGCT CCAAGACTCC 
CAGGACAAGT GTCACAAGAT GGAGCAGGAA ATGACCCGGT TACATCGCAG AGTGTCAGAG 
GTGGAGGCTG TGCTTAGTCA GAAGGAGGTG GAGCTGAAGG CCTCTGAGAC TCAGAGATCC 
CTCCTGGAGC AGGACCTTGC TACCTACATC ACAGAATGCA GTAGCTTAAA GCGAAGTTTG 
GAGCAAGCGC GGATGGAGGT GTCCCAGGAG GATGACAAAG CTCTGCAGCT TCTCCACGAC 
ATCCGAGAGC AGAGCCGGAA GCTCCAGGAG ATCAAGGAGC AGGAGTACCA GGCTCAGGTG 
GAGGAGATGA GGCTGATGAT GAATCAGCTG GAAGAAGACC TGGTGTCAGC CCGCAGACGC 
AGCGATCTCT ACGAGTCTGA GCTGAGGGAG TCTCGGCTTG CCGCCGAGGA ATTCAAGCGG 
AAGGCAAACG AATGTCAGCA CAAACTGATG AAGGTAGTCA GCCACCCTCC AAGAGGGGAC 
TCCGGGGGCA CGGCCCTGGA TGATCTTCAC AAGATGCAAG GCCACGCTGG CTTAACGAGT 
GCTAAGGACC AAGGGAAGCC TGAAGTGGGA GAATATTCCA AACTGGAGAA GATCAATGCT 
GAGCAGCAGC TGAAGATCCA GGAGCTCCAG GAGAAGCTGG AGAAGGCTGT AAAAGCCAGC 
ACAGAGGCCA CCGAGCTCCT GCAGAATATC CGCCAGGCAA AGGAGCGAGC AGAGCGGGAG 
CTAGAGAAGC TACACAACCG GGAAGACTCC TCCGAGGGCA TCAAAAAGAA GCTGGTGGAA 
GCCGAGGAAC GCCGCCACTC CCTGGAGAAC AAGGTAAAGA GACTAGAGAC CATGGAGCGT 
AGAGAGAACA GACTGAAGGA TGACATCCAG ACAAAGTCCG AACAGATCCA GCAGATGGCT 
GATAAAATTC TGGAGCTGGA GGAGAAACAT CGGGAGGCTC AGGTCTCAGC TCAACATCTA 
GAAGTACACT TGAAACAGAA AGAACAGCAC TACGAGGAAA AGATCAAGGT ATTGGACAAT 
CAGATAAAGA AGGACCTGGC CGACAAGGAG AGCCTGGAGA ACATGATGCA GAGACACGAG 
GAGGAGGCCC ACGAGAAGGG CAAGATCCTC AGCGAGCAGA AGGCGATGAT CAACGCGATG 
GATTCCAAGA TCCGATCCCT GGAGCAGAGG ATCGTGGAGC TGTCGGAAGC CAACAAGCTT 
GCGGCAAACA GCAGTCTCTT CACCCAGAGG AACATGAAGG CCCAGGAAGA GATGATCTCA 
GAACTCAGGC AGCAGAAATT TTACCTGGAG ACGCAGGCCG GAAAGCTGGA GGCCCAGAAC 
CGAAAGCTGG AAGAGCAACT GGAGAAGATC AGCCACCAAG ATCACAGTGA CAAGAGTCGG 
CTGCTGGAGC TGGAAACAAG GCTGAGGGAG GTCAGCCTGG AGCACGAGGA GCAGAAGCTG 
GAGCTGAAGC GGCAGCTCAC GGAGCTGCAG CTGTCCCTGC AGGAGCGCGA GTCCCAGCTG 
ACGGCCCTGC AGGCCGCCCG GGCAGCGCTG GAGAGCCAGC TCCGCCAGGC GAAGACAGAG 
CTGGAGGAGA CAACCGCGGA AGCGGAGGAG GAGATCCAGG CGCTCACGGC ACATCGAGAT 
GAGATCCAGC GCAAATTCGA TGCCCTTCGC AACAGCTGCA CGGTCATCAC CGACCTGGAG 
GAGCAGCTGA ACCAGCTCAC CGAGGACAAC GCCGAGCTCA ACAACCAAAA CTTCTACCTG 
TCCAAACAAC TCGATGAGGC TTCCGGGGCC AATGACGAGA TTGTGCAGCT GCGGAGTGAG 
GTGGACCATC TCCGCCGTGA GATCACGGAG CGGGAGATGC AGCTCACCAG CCAGAAGCAA 
GCACAACTCA GTGCTCCTGA TTTGCAGACG ATGGAGGCTC TGAAGACGAC ATGCACCATG 
CTGGAGGAGC AGGTCCTGGA CCTGGAGGCC CTGAACGACG AGCTGCTGGA GAAGGAGCGC 
CAGTGGGAGG CCTGGCGGAG CGTCCTCGGC GACGAGAAGT CCCAGTTCGA GTGTCGAGTT 
CGAGAGCTAC AGAGGATGCT GGACACCGAG AAGCAGAGCA GGGCTAGGGC CGATCAGCGG 
ATCACCGAGT CGCGCCAGGT GGTGGAGTTG GCGGTGAAGG AACACAAGGC CGAGATTCTT 
GCTCTGCAGC AGGCTCTCAA GGAGCAGAAG CTCAAAGCCG AGAGCCTGTC GGACAAGCTC 
AACGACCTGG AGAAGAAGCA CGCCATGCTG GAGATGAACG CCCGGAGCTT ACAGCAGAAA 
CTAGAGACAG AGCGGGAGCT CAAACAGAGG CTTCTGGAGG AGCAAGCCAA ATTGCAGCAG 
CAGATGGACC TGCAGAAGAA CCACATCTTC AGACTGACGC AAGGGCTGCA GGAGGCGCTG 
GACCGGGCGG ATCTGCTGAA GACAGAAAGG AGCGACCTGG AGTACCAGCT GGAAAACATT 
CAGGTTCTCT ACTCTCACGA GAAAGTGAAA ATGGAAGGCA CAATCTCTCA GCAAACCAAA 
CTCATTGATT TCCTGCAAGC CAAAATGGAC CAGCCTGCTA AAAAGAAAAA GGTTCCTCTG 
CAGTACAATG AGCTGAAGCT AGCCCTGGAA AAGGAGAAAG CCCGATGCGC GGAGCTGGAG 
GAGGCCCTTC AGAAGACCCG CATCGAACTC CGCTCTGCGC GGGAGGAAGC TGCCCACCGC 
AAAGCCACGG ACCACCCGCA CCCGTCTACG CCAGCCACTG CGAGGCAGCA GATCGCCATG 
TCTGCCATTG TGCGGTCGCC CGAGCACCAG CCCAGTGCCA TGAGCCTGCT TGCCCCACCA 
TCCAGCCGCA GAAAGGAGTC GTCAACTCCA GAGGAATTCA GCCGGCGTCT GAAAGAGCGC 
ATGCACCACA ACATCCCTCA CCGGTTTAAT GTGGGCCTGA ACATGAGAGC CACCAAGTGC 
GCCGTGTGTC TGGATACTGT GCACTTTGGA CGCCAGGCAT CCAAATGCCT CGAATGTCAA 
GTGATGTGTC ACCCCAAATG CTCCACCTGC TTGCCCGCTA CCTGTGGCCT GCCAGCTGAA 
TATGCCACAC ACTTCACTGA GGCCTTCTGC CGGGACAAAA TGAACTCCCC GGGGCTCCAG 
AGCAAGGAGC CTGGCAGCAG CTTGCACCTG GAAGGGTGGA TGAAAGTGCC CAGAAATAAC 
AAACGGGGAC AGCAAGGCTG GGACAGGAAG TACATTGTTC TGGAGGGGTC AAAAGTCCTC 
ATCTATGACA ATGAAGCCAG AGAAGCTGGA CAGAGGCCGG TGGAAGAATT TGAGCTGTGC 
CTTCCTGACG GGGACGTATC TATTCATGGT GCCGTTGGTG CTTCAGAACT TGCAAATACC 
GCCAAAGCAG ATGTCCCATA CATCCTGAAG ATGGAGTCTC ACCCACACAC CACCTGCTGG 
CCTGGGAGAA CCCTCTACTT GCTAGCACCC AGCTTCCCCG ACAAGCAGCG CTGGGTCACC 
GCCTTAGAAT CTGTCGTCGC AGGTGGGAGA GTTTCTAGGG AAAAGGCCGA AGCCGATGCT 
GCACGTGACC ATACTTCGAG TGAGCATCAG CCAGTGTGGG TTGAGAAATT ACTTGGAAAC 
TCTCTGCTGA AACTGGAGGG CGATGACCGG CTTGACATGA ACTGCACCCT GCCCTTCAGT 
GACCAGGTAG TGCTGGTGGG CACGGAGGAA GGCCTCTACG CCCTGAATGT CTTGAAAAAC 
TCCTTAACCC ACATCCCAGG GATTGGCGCA GTCTTCCAAA TTTACATCAT CAAGGACCTG 
GAGAAGCTGC TCATGATAGC AGGGGAAGAG CGGGCTCTGT GCCTGGTGGA CGTGAAGAAG 
GTGAAGCAGT CCCTGGCGCA GTCACACCTG CCTGCCCAGC CCGACGTCTC CCCCAACATA 
TTCGAAGCCG TCAAAGGCTG CCACTTGTTC GCTGCTGGCA AGATCGAGAA CAGCCTGTGC 
ATCTGCGCCG CTATGCCAAG CAAAGTCGTC ATCCTCCGCT ACAATGACAA CCTCAGCAAG 
TACTGCATCC GCAAGGAGAT CGAGACCTCA GAGCCCTGCA GCTGTATCCA CTTCACCAAC 
TACAGCATCC TCATTGGGAC CAACAAATTC TATGAGATCG ACATGAAGCA GTACACGCTT 
GACGAGTTCC TGGACAAGAA CGACCATTCC TTGGCACCTG CTGTGTTCGC CTCCTCGTCC 
AACAGCTTCC CTGTCTCCAT TGTGCAGGCG AACAGCGCCG GGCAGCGAGA AGAATACCTG 
CTGTGCTTCC ACGAATTTGG GGTGTTCGTG GATTCTTACG GAAGACGTAG CCGCACAGAT 
GATCTCAAGT GGAGTCGCTT ACCTCTGGCC TTCGCCTACA GAGAACCTTA TCTGTTTGTG 
ACTCACTTCA ACTCCCTGGA AGTCATTGAG ATCCAGGCCA GATCCTCACT GGGGAGCCCT 
GCCCGAGCAT ATCTGGAAAT TCCAAACCCT CGCTACCTGG GCCCCGCGAT TTCCTCCGGA 
GCGATTTACC TGGCCTCCTC ATACCAGGAC AAGTTAAGGG TCATATGCTG CAAAGGAAAC 
CTCGTGAAGG AGTCAGGCAC TGAGCAGCAC CGGGTGCCCT CCACCTCCCG CAGCAGCCCC 
AACAAGCGAG GCCCACCAAC ATACAACGAG CACATCACCA AACGCGTGGC CTCCAGCCCG 
GCGCCACCGG AAGGCCCCAG CCACCCCCGA GAGCCAAGCA CACCGCACCG CTACCGAGAC 
AGAGAGGGTC GGACAGAGCT GCGCAGGGAC AAGTCTCCAG GCCGCCCTCT GGAGCGGGAG 
AAGTCCCCAG GCCGAATGCT CAGCACTAGG AGAGAGCGGT CCCCAGGGAG ACTGTTTGAA 
GACAGCAGCA GGGGCCGGCT GCCTGCAGGA GCAGTGAGGA CCCCACTGTC CCAGGTTAAC 
AAGGTCTGGG ACCAGTCTTC AGTATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006530199.1
CDS	158..6484
Translation	MLKFKYGVRNPPEASASEPIASRASRLNLFFQGKPPLMTQQQMSALSREGMLDALFALFEECSQPALMKMKHVSSFVQKYSDTIAELRELQPSARDFEVRSLVGCGHFAEVQVVREKATGDVYAMKIMKKKALLAQEQVSFFEEERNILSRSTSPWIPQLQYAFQDKNNLYLVMEYQPGGDLLSLLNRYEDQLDESMIQFYLAELILAVHSVHQMGYVHRDIKPENILIDRTGHIKLVDFGSAAKMNSNKVDAKLPIGTPDYMAPEVLTVMNEDRRGTYGLDCDWWSVGVVAYEMVYGKTPFTEGTSARTFNNIMNFQRFLKFPDDPKVSSELLDLLQSLLCVQKERLKFEGLCCHPFFARTDWNNIRNSPPPFVPTLKSDDDTSNFDEPEKNSWVSSSVCQLSPSGFSGEELPFVGFSYSKALGYLGRSESVVSSLDSPAKVSSMEKKLLIKSKELQDSQDKCHKMEQEMTRLHRRVSEVEAVLSQKEVELKASETQRSLLEQDLATYITECSSLKRSLEQARMEVSQEDDKALQLLHDIREQSRKLQEIKEQEYQAQVEEMRLMMNQLEEDLVSARRRSDLYESELRESRLAAEEFKRKANECQHKLMKVVSHPPRGDSGGTALDDLHKMQGHAGLTSAKDQGKPEVGEYSKLEKINAEQQLKIQELQEKLEKAVKASTEATELLQNIRQAKERAERELEKLHNREDSSEGIKKKLVEAEERRHSLENKVKRLETMERRENRLKDDIQTKSEQIQQMADKILELEEKHREAQVSAQHLEVHLKQKEQHYEEKIKVLDNQIKKDLADKESLENMMQRHEEEAHEKGKILSEQKAMINAMDSKIRSLEQRIVELSEANKLAANSSLFTQRNMKAQEEMISELRQQKFYLETQAGKLEAQNRKLEEQLEKISHQDHSDKSRLLELETRLREVSLEHEEQKLELKRQLTELQLSLQERESQLTALQAARAALESQLRQAKTELEETTAEAEEEIQALTAHRDEIQRKFDALRNSCTVITDLEEQLNQLTEDNAELNNQNFYLSKQLDEASGANDEIVQLRSEVDHLRREITEREMQLTSQKQAQLSAPDLQTMEALKTTCTMLEEQVLDLEALNDELLEKERQWEAWRSVLGDEKSQFECRVRELQRMLDTEKQSRARADQRITESRQVVELAVKEHKAEILALQQALKEQKLKAESLSDKLNDLEKKHAMLEMNARSLQQKLETERELKQRLLEEQAKLQQQMDLQKNHIFRLTQGLQEALDRADLLKTERSDLEYQLENIQVLYSHEKVKMEGTISQQTKLIDFLQAKMDQPAKKKKVPLQYNELKLALEKEKARCAELEEALQKTRIELRSAREEAAHRKATDHPHPSTPATARQQIAMSAIVRSPEHQPSAMSLLAPPSSRRKESSTPEEFSRRLKERMHHNIPHRFNVGLNMRATKCAVCLDTVHFGRQASKCLECQVMCHPKCSTCLPATCGLPAEYATHFTEAFCRDKMNSPGLQSKEPGSSLHLEGWMKVPRNNKRGQQGWDRKYIVLEGSKVLIYDNEAREAGQRPVEEFELCLPDGDVSIHGAVGASELANTAKADVPYILKMESHPHTTCWPGRTLYLLAPSFPDKQRWVTALESVVAGGRVSREKAEADAARDHTSSEHQPVWVEKLLGNSLLKLEGDDRLDMNCTLPFSDQVVLVGTEEGLYALNVLKNSLTHIPGIGAVFQIYIIKDLEKLLMIAGEERALCLVDVKKVKQSLAQSHLPAQPDVSPNIFEAVKGCHLFAAGKIENSLCICAAMPSKVVILRYNDNLSKYCIRKEIETSEPCSCIHFTNYSILIGTNKFYEIDMKQYTLDEFLDKNDHSLAPAVFASSSNSFPVSIVQANSAGQREEYLLCFHEFGVFVDSYGRRSRTDDLKWSRLPLAFAYREPYLFVTHFNSLEVIEIQARSSLGSPARAYLEIPNPRYLGPAISSGAIYLASSYQDKLRVICCKGNLVKESGTEQHRVPSTSRSSPNKRGPPTYNEHITKRVASSPAPPEGPSHPREPSTPHRYRDREGRTELRRDKSPGRPLEREKSPGRMLSTRRERSPGRLFEDSSRGRLPAGAVRTPLSQVNKVWDQSSV

Target ORF information:

RefSeq Version	XM_006530136.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus citron (Cit), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006530136.4

ORF Insert Sequence:

ATGTTGAAGT TCAAGTATGG TGTGCGGAAC CCGCCGGAGG CCAGTGCCTC CGAGCCCATT 
GCCAGTCGGG CCTCCAGGCT AAATCTCTTC TTCCAGGGGA AACCGCCCCT CATGACTCAA 
CAGCAGATGT CTGCTCTTTC CCGGGAAGGG ATGCTAGACG CCCTCTTCGC TCTCTTTGAA 
GAGTGCAGCC AACCCGCCCT GATGAAGATG AAGCACGTGA GCAGCTTTGT CCAGAAGTAT 
TCCGACACCA TAGCCGAGTT GCGGGAGCTG CAGCCGTCGG CGAGAGACTT CGAAGTTCGA 
AGCCTTGTGG GCTGTGGTCA CTTCGCTGAA GTGCAGGTGG TTAGAGAGAA GGCGACCGGG 
GACGTCTATG CCATGAAAAT CATGAAGAAG AAGGCTTTGC TGGCCCAGGA ACAGGTTTCA 
TTTTTCGAGG AGGAGAGGAA CATATTATCT CGGAGCACGA GTCCTTGGAT CCCCCAGTTA 
CAGTACGCCT TTCAGGACAA AAATAACCTT TACCTGGTCA TGGAATATCA GCCTGGAGGG 
GATTTGCTGT CGCTTCTGAA CAGATACGAG GACCAATTAG ATGAGAGCAT GATCCAGTTT 
TACCTTGCTG AGCTGATTTT GGCTGTCCAC AGCGTGCACC AGATGGGATA TGTGCATCGA 
GACATCAAGC CCGAGAACAT CCTCATCGAC CGGACGGGAC ACATCAAGCT GGTGGATTTT 
GGATCAGCCG CTAAGATGAA TTCAAATAAG GTGGATGCCA AACTCCCCAT TGGGACCCCG 
GATTACATGG CTCCGGAAGT GTTGACCGTG ATGAACGAGG ACCGAAGGGG CACATACGGC 
TTGGACTGTG ACTGGTGGTC TGTCGGAGTT GTTGCTTATG AGATGGTTTA TGGGAAGACC 
CCATTCACAG AGGGAACCTC CGCCCGGACC TTCAACAACA TCATGAACTT CCAGCGGTTT 
TTGAAGTTCC CAGATGACCC CAAAGTTAGC AGTGAGCTCC TTGATCTGCT TCAGAGTCTG 
CTGTGTGTCC AGAAAGAGAG ACTGAAGTTC GAGGGTCTCT GCTGCCACCC TTTCTTTGCC 
AGAACGGACT GGAACAACAT CCGTAACTCT CCTCCCCCCT TCGTCCCCAC CCTCAAGTCT 
GACGATGACA CCTCCAATTT TGATGAACCA GAGAAGAATT CGTGGGTTTC ATCCTCTGTG 
TGCCAGCTGA GCCCCTCGGG CTTCTCAGGC GAAGAGCTGC CGTTTGTGGG ATTTTCGTAC 
AGCAAGGCAC TGGGGTATCT TGGTAGATCT GAGTCTGTCG TGTCGAGTCT GGACTCCCCT 
GCCAAGGTTA GCTCCATGGA AAAGAAACTT CTCATCAAAA GCAAAGAGCT CCAAGACTCC 
CAGGACAAGT GTCACAAGAT GGAGCAGGAA ATGACCCGGT TACATCGCAG AGTGTCAGAG 
GTGGAGGCTG TGCTTAGTCA GAAGGAGGTG GAGCTGAAGG CCTCTGAGAC TCAGAGATCC 
CTCCTGGAGC AGGACCTTGC TACCTACATC ACAGAATGCA GTAGCTTAAA GCGAAGTTTG 
GAGCAAGCGC GGATGGAGGT GTCCCAGGAG GATGACAAAG CTCTGCAGCT TCTCCACGAC 
ATCCGAGAGC AGAGCCGGAA GCTCCAGGAG ATCAAGGAGC AGGAGTACCA GGCTCAGGTG 
GAGGAGATGA GGCTGATGAT GAATCAGCTG GAAGAAGACC TGGTGTCAGC CCGCAGACGC 
AGCGATCTCT ACGAGTCTGA GCTGAGGGAG TCTCGGCTTG CCGCCGAGGA ATTCAAGCGG 
AAGGCAAACG AATGTCAGCA CAAACTGATG AAGGTAGTCA GCCACCCTCC AAGAGGGGAC 
TCCGGGGGCA CGGCCCTGGA TGATCTTCAC AAGATGCAAG GCCACGCTGG CTTAACGAGT 
GCTAAGGACC AAGGGAAGCC TGAAGTGGGA GAATATTCCA AACTGGAGAA GATCAATGCT 
GAGCAGCAGC TGAAGATCCA GGAGCTCCAG GAGAAGCTGG AGAAGGCTGT AAAAGCCAGC 
ACAGAGGCCA CCGAGCTCCT GCAGAATATC CGCCAGGCAA AGGAGCGAGC AGAGCGGGAG 
CTAGAGAAGC TACACAACCG GGAAGACTCC TCCGAGGGCA TCAAAAAGAA GCTGGTGGAA 
GCCGAGGAAC GCCGCCACTC CCTGGAGAAC AAGGTAAAGA GACTAGAGAC CATGGAGCGT 
AGAGAGAACA GACTGAAGGA TGACATCCAG ACAAAGTCCG AACAGATCCA GCAGATGGCT 
GATAAAATTC TGGAGCTGGA GGAGAAACAT CGGGAGGCTC AGGTCTCAGC TCAACATCTA 
GAAGTACACT TGAAACAGAA AGAACAGCAC TACGAGGAAA AGATCAAGGT ATTGGACAAT 
CAGATAAAGA AGGACCTGGC CGACAAGGAG AGCCTGGAGA ACATGATGCA GAGACACGAG 
GAGGAGGCCC ACGAGAAGGG CAAGATCCTC AGCGAGCAGA AGGCGATGAT CAACGCGATG 
GATTCCAAGA TCCGATCCCT GGAGCAGAGG ATCGTGGAGC TGTCGGAAGC CAACAAGCTT 
GCGGCAAACA GCAGTCTCTT CACCCAGAGG AACATGAAGG CCCAGGAAGA GATGATCTCA 
GAACTCAGGC AGCAGAAATT TTACCTGGAG ACGCAGGCCG GAAAGCTGGA GGCCCAGAAC 
CGAAAGCTGG AAGAGCAACT GGAGAAGATC AGCCACCAAG ATCACAGTGA CAAGAGTCGG 
CTGCTGGAGC TGGAAACAAG GCTGAGGGAG GTCAGCCTGG AGCACGAGGA GCAGAAGCTG 
GAGCTGAAGC GGCAGCTCAC GGAGCTGCAG CTGTCCCTGC AGGAGCGCGA GTCCCAGCTG 
ACGGCCCTGC AGGCCGCCCG GGCAGCGCTG GAGAGCCAGC TCCGCCAGGC GAAGACAGAG 
CTGGAGGAGA CAACCGCGGA AGCGGAGGAG GAGATCCAGG CGCTCACGGC ACATCGAGAT 
GAGATCCAGC GCAAATTCGA TGCCCTTCGC AACAGCTGCA CGGTCATCAC CGACCTGGAG 
GAGCAGCTGA ACCAGCTCAC CGAGGACAAC GCCGAGCTCA ACAACCAAAA CTTCTACCTG 
TCCAAACAAC TCGATGAGGC TTCCGGGGCC AATGACGAGA TTGTGCAGCT GCGGAGTGAG 
GTGGACCATC TCCGCCGTGA GATCACGGAG CGGGAGATGC AGCTCACCAG CCAGAAGCAA 
GCACAACTCA GTGCTCCTGA TTTGCAGACG ATGGAGGCTC TGAAGACGAC ATGCACCATG 
CTGGAGGAGC AGGTCCTGGA CCTGGAGGCC CTGAACGACG AGCTGCTGGA GAAGGAGCGC 
CAGTGGGAGG CCTGGCGGAG CGTCCTCGGC GACGAGAAGT CCCAGTTCGA GTGTCGAGTT 
CGAGAGCTAC AGAGGATGCT GGACACCGAG AAGCAGAGCA GGGCTAGGGC CGATCAGCGG 
ATCACCGAGT CGCGCCAGGT GGTGGAGTTG GCGGTGAAGG AACACAAGGC CGAGATTCTT 
GCTCTGCAGC AGGCTCTCAA GGAGCAGAAG CTCAAAGCCG AGAGCCTGTC GGACAAGCTC 
AACGACCTGG AGAAGAAGCA CGCCATGCTG GAGATGAACG CCCGGAGCTT ACAGCAGAAA 
CTAGAGACAG AGCGGGAGCT CAAACAGAGG CTTCTGGAGG AGCAAGCCAA ATTGCAGCAG 
CAGATGGACC TGCAGAAGAA CCACATCTTC AGACTGACGC AAGGGCTGCA GGAGGCGCTG 
GACCGGGCGG ATCTGCTGAA GACAGAAAGG AGCGACCTGG AGTACCAGCT GGAAAACATT 
CAGGTTCTCT ACTCTCACGA GAAAGTGAAA ATGGAAGGCA CAATCTCTCA GCAAACCAAA 
CTCATTGATT TCCTGCAAGC CAAAATGGAC CAGCCTGCTA AAAAGAAAAA GGTTCCTCTG 
CAGTACAATG AGCTGAAGCT AGCCCTGGAA AAGGAGAAAG CCCGATGCGC GGAGCTGGAG 
GAGGCCCTTC AGAAGACCCG CATCGAACTC CGCTCTGCGC GGGAGGAAGC TGCCCACCGC 
AAAGCCACGG ACCACCCGCA CCCGTCTACG CCAGCCACTG CGAGGCAGCA GATCGCCATG 
TCTGCCATTG TGCGGTCGCC CGAGCACCAG CCCAGTGCCA TGAGCCTGCT TGCCCCACCA 
TCCAGCCGCA GAAAGGAGTC GTCAACTCCA GAGGAATTCA GCCGGCGTCT GAAAGAGCGC 
ATGCACCACA ACATCCCTCA CCGGTTTAAT GTGGGCCTGA ACATGAGAGC CACCAAGTGC 
GCCGTGTGTC TGGATACTGT GCACTTTGGA CGCCAGGCAT CCAAATGCCT CGAATGTCAA 
GTGATGTGTC ACCCCAAATG CTCCACCTGC TTGCCCGCTA CCTGTGGCCT GCCAGCTGAA 
TATGCCACAC ACTTCACTGA GGCCTTCTGC CGGGACAAAA TGAACTCCCC GGGGCTCCAG 
AGCAAGGAGC CTGGCAGCAG CTTGCACCTG GAAGGGTGGA TGAAAGTGCC CAGAAATAAC 
AAACGGGGAC AGCAAGGCTG GGACAGGAAG TACATTGTTC TGGAGGGGTC AAAAGTCCTC 
ATCTATGACA ATGAAGCCAG AGAAGCTGGA CAGAGGCCGG TGGAAGAATT TGAGCTGTGC 
CTTCCTGACG GGGACGTATC TATTCATGGT GCCGTTGGTG CTTCAGAACT TGCAAATACC 
GCCAAAGCAG ATGTCCCATA CATCCTGAAG ATGGAGTCTC ACCCACACAC CACCTGCTGG 
CCTGGGAGAA CCCTCTACTT GCTAGCACCC AGCTTCCCCG ACAAGCAGCG CTGGGTCACC 
GCCTTAGAAT CTGTCGTCGC AGGTGGGAGA GTTTCTAGGG AAAAGGCCGA AGCCGATGCT 
GCACGTGACC ATACTTCGAG TGAGCATCAG CCAGTGTGGG TTGAGAAATT ACTTGGAAAC 
TCTCTGCTGA AACTGGAGGG CGATGACCGG CTTGACATGA ACTGCACCCT GCCCTTCAGT 
GACCAGGTAG TGCTGGTGGG CACGGAGGAA GGCCTCTACG CCCTGAATGT CTTGAAAAAC 
TCCTTAACCC ACATCCCAGG GATTGGCGCA GTCTTCCAAA TTTACATCAT CAAGGACCTG 
GAGAAGCTGC TCATGATAGC AGGGGAAGAG CGGGCTCTGT GCCTGGTGGA CGTGAAGAAG 
GTGAAGCAGT CCCTGGCGCA GTCACACCTG CCTGCCCAGC CCGACGTCTC CCCCAACATA 
TTCGAAGCCG TCAAAGGCTG CCACTTGTTC GCTGCTGGCA AGATCGAGAA CAGCCTGTGC 
ATCTGCGCCG CTATGCCAAG CAAAGTCGTC ATCCTCCGCT ACAATGACAA CCTCAGCAAG 
TACTGCATCC GCAAGGAGAT CGAGACCTCA GAGCCCTGCA GCTGTATCCA CTTCACCAAC 
TACAGCATCC TCATTGGGAC CAACAAATTC TATGAGATCG ACATGAAGCA GTACACGCTT 
GACGAGTTCC TGGACAAGAA CGACCATTCC TTGGCACCTG CTGTGTTCGC CTCCTCGTCC 
AACAGCTTCC CTGTCTCCAT TGTGCAGGCG AACAGCGCCG GGCAGCGAGA AGAATACCTG 
CTGTGCTTCC ACGAATTTGG GGTGTTCGTG GATTCTTACG GAAGACGTAG CCGCACAGAT 
GATCTCAAGT GGAGTCGCTT ACCTCTGGCC TTCGCCTACA GAGAACCTTA TCTGTTTGTG 
ACTCACTTCA ACTCCCTGGA AGTCATTGAG ATCCAGGCCA GATCCTCACT GGGGAGCCCT 
GCCCGAGCAT ATCTGGAAAT TCCAAACCCT CGCTACCTGG GCCCCGCGAT TTCCTCCGGA 
GCGATTTACC TGGCCTCCTC ATACCAGGAC AAGTTAAGGG TCATATGCTG CAAAGGAAAC 
CTCGTGAAGG AGTCAGGCAC TGAGCAGCAC CGGGTGCCCT CCACCTCCCG CAGCAGCCCC 
AACAAGCGAG GCCCACCAAC ATACAACGAG CACATCACCA AACGCGTGGC CTCCAGCCCG 
GCGCCACCGG AAGGCCCCAG CCACCCCCGA GAGCCAAGCA CACCGCACCG CTACCGAGAC 
AGAGAGGGTC GGACAGAGCT GCGCAGGGAC AAGTCTCCAG GCCGCCCTCT GGAGCGGGAG 
AAGTCCCCAG GCCGAATGCT CAGCACTAGG AGAGAGCGGT CCCCAGGGAG ACTGTTTGAA 
GACAGCAGCA GGGGCCGGCT GCCTGCAGGA GCAGTGAGGA CCCCACTGTC CCAGGTTAAC 
AAGGTCTGGG ACCAGTCTTC AGTATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cit ( XM_006530137.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006530200.1 Mus musculus citron (Cit), transcript variant X8, mRNA.

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CloneID	OMu35778
Clone ID Related Accession (Same CDS sequence)	XM_006530137.4 , XM_006530137.3
Accession Version	XM_006530137.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6327bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	citron Rho-interacting kinase isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000071.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006530137.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTGAAGT TCAAGTATGG TGTGCGGAAC CCGCCGGAGG CCAGTGCCTC CGAGCCCATT 
GCCAGTCGGG CCTCCAGGCT AAATCTCTTC TTCCAGGGGA AACCGCCCCT CATGACTCAA 
CAGCAGATGT CTGCTCTTTC CCGGGAAGGG ATGCTAGACG CCCTCTTCGC TCTCTTTGAA 
GAGTGCAGCC AACCCGCCCT GATGAAGATG AAGCACGTGA GCAGCTTTGT CCAGAAGTAT 
TCCGACACCA TAGCCGAGTT GCGGGAGCTG CAGCCGTCGG CGAGAGACTT CGAAGTTCGA 
AGCCTTGTGG GCTGTGGTCA CTTCGCTGAA GTGCAGGTGG TTAGAGAGAA GGCGACCGGG 
GACGTCTATG CCATGAAAAT CATGAAGAAG AAGGCTTTGC TGGCCCAGGA ACAGGTTTCA 
TTTTTCGAGG AGGAGAGGAA CATATTATCT CGGAGCACGA GTCCTTGGAT CCCCCAGTTA 
CAGTACGCCT TTCAGGACAA AAATAACCTT TACCTGGTCA TGGAATATCA GCCTGGAGGG 
GATTTGCTGT CGCTTCTGAA CAGATACGAG GACCAATTAG ATGAGAGCAT GATCCAGTTT 
TACCTTGCTG AGCTGATTTT GGCTGTCCAC AGCGTGCACC AGATGGGATA TGTGCATCGA 
GACATCAAGC CCGAGAACAT CCTCATCGAC CGGACGGGAC ACATCAAGCT GGTGGATTTT 
GGATCAGCCG CTAAGATGAA TTCAAATAAG GTGGATGCCA AACTCCCCAT TGGGACCCCG 
GATTACATGG CTCCGGAAGT GTTGACCGTG ATGAACGAGG ACCGAAGGGG CACATACGGC 
TTGGACTGTG ACTGGTGGTC TGTCGGAGTT GTTGCTTATG AGATGGTTTA TGGGAAGACC 
CCATTCACAG AGGGAACCTC CGCCCGGACC TTCAACAACA TCATGAACTT CCAGCGGTTT 
TTGAAGTTCC CAGATGACCC CAAAGTTAGC AGTGAGCTCC TTGATCTGCT TCAGAGTCTG 
CTGTGTGTCC AGAAAGAGAG ACTGAAGTTC GAGGGTCTCT GCTGCCACCC TTTCTTTGCC 
AGAACGGACT GGAACAACAT CCGTAACTCT CCTCCCCCCT TCGTCCCCAC CCTCAAGTCT 
GACGATGACA CCTCCAATTT TGATGAACCA GAGAAGAATT CGTGGGTTTC ATCCTCTGTG 
TGCCAGCTGA GCCCCTCGGG CTTCTCAGGC GAAGAGCTGC CGTTTGTGGG ATTTTCGTAC 
AGCAAGGCAC TGGGGTATCT TGGTAGATCT GAGTCTGTCG TGTCGAGTCT GGACTCCCCT 
GCCAAGGTTA GCTCCATGGA AAAGAAACTT CTCATCAAAA GCAAAGAGCT CCAAGACTCC 
CAGGACAAGT GTCACAAGAT GGAGCAGGAA ATGACCCGGT TACATCGCAG AGTGTCAGAG 
GTGGAGGCTG TGCTTAGTCA GAAGGAGGTG GAGCTGAAGG CCTCTGAGAC TCAGAGATCC 
CTCCTGGAGC AGGACCTTGC TACCTACATC ACAGAATGCA GTAGCTTAAA GCGAAGTTTG 
GAGCAAGCGC GGATGGAGGT GTCCCAGGAG GATGACAAAG CTCTGCAGCT TCTCCACGAC 
ATCCGAGAGC AGAGCCGGAA GCTCCAGGAG ATCAAGGAGC AGGAGTACCA GGCTCAGGTG 
GAGGAGATGA GGCTGATGAT GAATCAGCTG GAAGAAGACC TGGTGTCAGC CCGCAGACGC 
AGCGATCTCT ACGAGTCTGA GCTGAGGGAG TCTCGGCTTG CCGCCGAGGA ATTCAAGCGG 
AAGGCAAACG AATGTCAGCA CAAACTGATG AAGGTAGTCA GCCACCCTCC AAGAGGGGAC 
TCCGGGGGCA CGGCCCTGGA TGATCTTCAC AAGATGCAAG GCCACGCTGG CTTAACGAGT 
GCTAAGGACC AAGGGAAGCC TGAAGTGGGA GAATATTCCA AACTGGAGAA GATCAATGCT 
GAGCAGCAGC TGAAGATCCA GGAGCTCCAG GAGAAGCTGG AGAAGGCTGT AAAAGCCAGC 
ACAGAGGCCA CCGAGCTCCT GCAGAATATC CGCCAGGCAA AGGAGCGAGC AGAGCGGGAG 
CTAGAGAAGC TACACAACCG GGAAGACTCC TCCGAGGGCA TCAAAAAGAA GCTGGTGGAA 
GCCGAGGAAC GCCGCCACTC CCTGGAGAAC AAGGTAAAGA GACTAGAGAC CATGGAGCGT 
AGAGAGAACA GACTGAAGGA TGACATCCAG ACAAAGTCCG AACAGATCCA GCAGATGGCT 
GATAAAATTC TGGAGCTGGA GGAGAAACAT CGGGAGGCTC AGGTCTCAGC TCAACATCTA 
GAAGTACACT TGAAACAGAA AGAACAGCAC TACGAGGAAA AGATCAAGGT ATTGGACAAT 
CAGATAAAGA AGGACCTGGC CGACAAGGAG AGCCTGGAGA ACATGATGCA GAGACACGAG 
GAGGAGGCCC ACGAGAAGGG CAAGATCCTC AGCGAGCAGA AGGCGATGAT CAACGCGATG 
GATTCCAAGA TCCGATCCCT GGAGCAGAGG ATCGTGGAGC TGTCGGAAGC CAACAAGCTT 
GCGGCAAACA GCAGTCTCTT CACCCAGAGG AACATGAAGG CCCAGGAAGA GATGATCTCA 
GAACTCAGGC AGCAGAAATT TTACCTGGAG ACGCAGGCCG GAAAGCTGGA GGCCCAGAAC 
CGAAAGCTGG AAGAGCAACT GGAGAAGATC AGCCACCAAG ATCACAGTGA CAAGAGTCGG 
CTGCTGGAGC TGGAAACAAG GCTGAGGGAG GTCAGCCTGG AGCACGAGGA GCAGAAGCTG 
GAGCTGAAGC GGCAGCTCAC GGAGCTGCAG CTGTCCCTGC AGGAGCGCGA GTCCCAGCTG 
ACGGCCCTGC AGGCCGCCCG GGCAGCGCTG GAGAGCCAGC TCCGCCAGGC GAAGACAGAG 
CTGGAGGAGA CAACCGCGGA AGCGGAGGAG GAGATCCAGG CGCTCACGGC ACATCGAGAT 
GAGATCCAGC GCAAATTCGA TGCCCTTCGC AACAGCTGCA CGGTCATCAC CGACCTGGAG 
GAGCAGCTGA ACCAGCTCAC CGAGGACAAC GCCGAGCTCA ACAACCAAAA CTTCTACCTG 
TCCAAACAAC TCGATGAGGC TTCCGGGGCC AATGACGAGA TTGTGCAGCT GCGGAGTGAG 
GTGGACCATC TCCGCCGTGA GATCACGGAG CGGGAGATGC AGCTCACCAG CCAGAAGCAA 
GCACAACTCA GTGCTCCTGA TTTGCAGACG ATGGAGGCTC TGAAGACGAC ATGCACCATG 
CTGGAGGAGC AGGTCCTGGA CCTGGAGGCC CTGAACGACG AGCTGCTGGA GAAGGAGCGC 
CAGTGGGAGG CCTGGCGGAG CGTCCTCGGC GACGAGAAGT CCCAGTTCGA GTGTCGAGTT 
CGAGAGCTAC AGAGGATGCT GGACACCGAG AAGCAGAGCA GGGCTAGGGC CGATCAGCGG 
ATCACCGAGT CGCGCCAGGT GGTGGAGTTG GCGGTGAAGG AACACAAGGC CGAGATTCTT 
GCTCTGCAGC AGGCTCTCAA GGAGCAGAAG CTCAAAGCCG AGAGCCTGTC GGACAAGCTC 
AACGACCTGG AGAAGAAGCA CGCCATGCTG GAGATGAACG CCCGGAGCTT ACAGCAGAAA 
CTAGAGACAG AGCGGGAGCT CAAACAGAGG CTTCTGGAGG AGCAAGCCAA ATTGCAGCAG 
CAGATGGACC TGCAGAAGAA CCACATCTTC AGACTGACGC AAGGGCTGCA GGAGGCGCTG 
GACCGGGCGG ATCTGCTGAA GACAGAAAGG AGCGACCTGG AGTACCAGCT GGAAAACATT 
CAGGTTCTCT ACTCTCACGA GAAAGTGAAA ATGGAAGGCA CAATCTCTCA GCAAACCAAA 
CTCATTGATT TCCTGCAAGC CAAAATGGAC CAGCCTGCTA AAAAGAAAAA GGGTTTATTT 
AGTCGACGGA AAGAGGACCC TGCTTTGCCC ACACAGGTTC CTCTGCAGTA CAATGAGCTG 
AAGCTAGCCC TGGAAAAGGA GAAAGCCCGA TGCGCGGAGC TGGAGGAGGC CCTTCAGAAG 
ACCCGCATCG AACTCCGCTC TGCGCGGGAG GAAGCTGCCC ACCGCAAAGC CACGGACCAC 
CCGCACCCGT CTACGCCAGC CACTGCGAGG CAGCAGATCG CCATGTCTGC CATTGTGCGG 
TCGCCCGAGC ACCAGCCCAG TGCCATGAGC CTGCTTGCCC CACCATCCAG CCGCAGAAAG 
GAGTCGTCAA CTCCAGAGGA ATTCAGCCGG CGTCTGAAAG AGCGCATGCA CCACAACATC 
CCTCACCGGT TTAATGTGGG CCTGAACATG AGAGCCACCA AGTGCGCCGT GTGTCTGGAT 
ACTGTGCACT TTGGACGCCA GGCATCCAAA TGCCTCGAAT GTCAAGTGAT GTGTCACCCC 
AAATGCTCCA CCTGCTTGCC CGCTACCTGT GGCCTGCCAG CTGAATATGC CACACACTTC 
ACTGAGGCCT TCTGCCGGGA CAAAATGAAC TCCCCGGGGC TCCAGAGCAA GGAGCCTGGC 
AGCAGCTTGC ACCTGGAAGG GTGGATGAAA GTGCCCAGAA ATAACAAACG GGGACAGCAA 
GGCTGGGACA GGAAGTACAT TGTTCTGGAG GGGTCAAAAG TCCTCATCTA TGACAATGAA 
GCCAGAGAAG CTGGACAGAG GCCGGTGGAA GAATTTGAGC TGTGCCTTCC TGACGGGGAC 
GTATCTATTC ATGGTGCCGT TGGTGCTTCA GAACTTGCAA ATACCGCCAA AGCAGATGTC 
CCATACATCC TGAAGATGGA GTCTCACCCA CACACCACCT GCTGGCCTGG GAGAACCCTC 
TACTTGCTAG CACCCAGCTT CCCCGACAAG CAGCGCTGGG TCACCGCCTT AGAATCTGTC 
GTCGCAGGTG GGAGAGTTTC TAGGGAAAAG GCCGAAGCCG ATGCTAAATT ACTTGGAAAC 
TCTCTGCTGA AACTGGAGGG CGATGACCGG CTTGACATGA ACTGCACCCT GCCCTTCAGT 
GACCAGGTAG TGCTGGTGGG CACGGAGGAA GGCCTCTACG CCCTGAATGT CTTGAAAAAC 
TCCTTAACCC ACATCCCAGG GATTGGCGCA GTCTTCCAAA TTTACATCAT CAAGGACCTG 
GAGAAGCTGC TCATGATAGC AGGGGAAGAG CGGGCTCTGT GCCTGGTGGA CGTGAAGAAG 
GTGAAGCAGT CCCTGGCGCA GTCACACCTG CCTGCCCAGC CCGACGTCTC CCCCAACATA 
TTCGAAGCCG TCAAAGGCTG CCACTTGTTC GCTGCTGGCA AGATCGAGAA CAGCCTGTGC 
ATCTGCGCCG CTATGCCAAG CAAAGTCGTC ATCCTCCGCT ACAATGACAA CCTCAGCAAG 
TACTGCATCC GCAAGGAGAT CGAGACCTCA GAGCCCTGCA GCTGTATCCA CTTCACCAAC 
TACAGCATCC TCATTGGGAC CAACAAATTC TATGAGATCG ACATGAAGCA GTACACGCTT 
GACGAGTTCC TGGACAAGAA CGACCATTCC TTGGCACCTG CTGTGTTCGC CTCCTCGTCC 
AACAGCTTCC CTGTCTCCAT TGTGCAGGCG AACAGCGCCG GGCAGCGAGA AGAATACCTG 
CTGTGCTTCC ACGAATTTGG GGTGTTCGTG GATTCTTACG GAAGACGTAG CCGCACAGAT 
GATCTCAAGT GGAGTCGCTT ACCTCTGGCC TTCGCCTACA GAGAACCTTA TCTGTTTGTG 
ACTCACTTCA ACTCCCTGGA AGTCATTGAG ATCCAGGCCA GATCCTCACT GGGGAGCCCT 
GCCCGAGCAT ATCTGGAAAT TCCAAACCCT CGCTACCTGG GCCCCGCGAT TTCCTCCGGA 
GCGATTTACC TGGCCTCCTC ATACCAGGAC AAGTTAAGGG TCATATGCTG CAAAGGAAAC 
CTCGTGAAGG AGTCAGGCAC TGAGCAGCAC CGGGTGCCCT CCACCTCCCG CAGCAGCCCC 
AACAAGCGAG GCCCACCAAC ATACAACGAG CACATCACCA AACGCGTGGC CTCCAGCCCG 
GCGCCACCGG AAGGCCCCAG CCACCCCCGA GAGCCAAGCA CACCGCACCG CTACCGAGAC 
AGAGAGGGTC GGACAGAGCT GCGCAGGGAC AAGTCTCCAG GCCGCCCTCT GGAGCGGGAG 
AAGTCCCCAG GCCGAATGCT CAGCACTAGG AGAGAGCGGT CCCCAGGGAG ACTGTTTGAA 
GACAGCAGCA GGGGCCGGCT GCCTGCAGGA GCAGTGAGGA CCCCACTGTC CCAGGTTAAC 
AAGGTCTGGG ACCAGTCTTC AGTATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006530200.1
CDS	158..6484
Translation	MLKFKYGVRNPPEASASEPIASRASRLNLFFQGKPPLMTQQQMSALSREGMLDALFALFEECSQPALMKMKHVSSFVQKYSDTIAELRELQPSARDFEVRSLVGCGHFAEVQVVREKATGDVYAMKIMKKKALLAQEQVSFFEEERNILSRSTSPWIPQLQYAFQDKNNLYLVMEYQPGGDLLSLLNRYEDQLDESMIQFYLAELILAVHSVHQMGYVHRDIKPENILIDRTGHIKLVDFGSAAKMNSNKVDAKLPIGTPDYMAPEVLTVMNEDRRGTYGLDCDWWSVGVVAYEMVYGKTPFTEGTSARTFNNIMNFQRFLKFPDDPKVSSELLDLLQSLLCVQKERLKFEGLCCHPFFARTDWNNIRNSPPPFVPTLKSDDDTSNFDEPEKNSWVSSSVCQLSPSGFSGEELPFVGFSYSKALGYLGRSESVVSSLDSPAKVSSMEKKLLIKSKELQDSQDKCHKMEQEMTRLHRRVSEVEAVLSQKEVELKASETQRSLLEQDLATYITECSSLKRSLEQARMEVSQEDDKALQLLHDIREQSRKLQEIKEQEYQAQVEEMRLMMNQLEEDLVSARRRSDLYESELRESRLAAEEFKRKANECQHKLMKVVSHPPRGDSGGTALDDLHKMQGHAGLTSAKDQGKPEVGEYSKLEKINAEQQLKIQELQEKLEKAVKASTEATELLQNIRQAKERAERELEKLHNREDSSEGIKKKLVEAEERRHSLENKVKRLETMERRENRLKDDIQTKSEQIQQMADKILELEEKHREAQVSAQHLEVHLKQKEQHYEEKIKVLDNQIKKDLADKESLENMMQRHEEEAHEKGKILSEQKAMINAMDSKIRSLEQRIVELSEANKLAANSSLFTQRNMKAQEEMISELRQQKFYLETQAGKLEAQNRKLEEQLEKISHQDHSDKSRLLELETRLREVSLEHEEQKLELKRQLTELQLSLQERESQLTALQAARAALESQLRQAKTELEETTAEAEEEIQALTAHRDEIQRKFDALRNSCTVITDLEEQLNQLTEDNAELNNQNFYLSKQLDEASGANDEIVQLRSEVDHLRREITEREMQLTSQKQAQLSAPDLQTMEALKTTCTMLEEQVLDLEALNDELLEKERQWEAWRSVLGDEKSQFECRVRELQRMLDTEKQSRARADQRITESRQVVELAVKEHKAEILALQQALKEQKLKAESLSDKLNDLEKKHAMLEMNARSLQQKLETERELKQRLLEEQAKLQQQMDLQKNHIFRLTQGLQEALDRADLLKTERSDLEYQLENIQVLYSHEKVKMEGTISQQTKLIDFLQAKMDQPAKKKKGLFSRRKEDPALPTQVPLQYNELKLALEKEKARCAELEEALQKTRIELRSAREEAAHRKATDHPHPSTPATARQQIAMSAIVRSPEHQPSAMSLLAPPSSRRKESSTPEEFSRRLKERMHHNIPHRFNVGLNMRATKCAVCLDTVHFGRQASKCLECQVMCHPKCSTCLPATCGLPAEYATHFTEAFCRDKMNSPGLQSKEPGSSLHLEGWMKVPRNNKRGQQGWDRKYIVLEGSKVLIYDNEAREAGQRPVEEFELCLPDGDVSIHGAVGASELANTAKADVPYILKMESHPHTTCWPGRTLYLLAPSFPDKQRWVTALESVVAGGRVSREKAEADAKLLGNSLLKLEGDDRLDMNCTLPFSDQVVLVGTEEGLYALNVLKNSLTHIPGIGAVFQIYIIKDLEKLLMIAGEERALCLVDVKKVKQSLAQSHLPAQPDVSPNIFEAVKGCHLFAAGKIENSLCICAAMPSKVVILRYNDNLSKYCIRKEIETSEPCSCIHFTNYSILIGTNKFYEIDMKQYTLDEFLDKNDHSLAPAVFASSSNSFPVSIVQANSAGQREEYLLCFHEFGVFVDSYGRRSRTDDLKWSRLPLAFAYREPYLFVTHFNSLEVIEIQARSSLGSPARAYLEIPNPRYLGPAISSGAIYLASSYQDKLRVICCKGNLVKESGTEQHRVPSTSRSSPNKRGPPTYNEHITKRVASSPAPPEGPSHPREPSTPHRYRDREGRTELRRDKSPGRPLEREKSPGRMLSTRRERSPGRLFEDSSRGRLPAGAVRTPLSQVNKVWDQSSV

Target ORF information:

RefSeq Version	XM_006530137.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus citron (Cit), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006530137.4

ORF Insert Sequence:

ATGTTGAAGT TCAAGTATGG TGTGCGGAAC CCGCCGGAGG CCAGTGCCTC CGAGCCCATT 
GCCAGTCGGG CCTCCAGGCT AAATCTCTTC TTCCAGGGGA AACCGCCCCT CATGACTCAA 
CAGCAGATGT CTGCTCTTTC CCGGGAAGGG ATGCTAGACG CCCTCTTCGC TCTCTTTGAA 
GAGTGCAGCC AACCCGCCCT GATGAAGATG AAGCACGTGA GCAGCTTTGT CCAGAAGTAT 
TCCGACACCA TAGCCGAGTT GCGGGAGCTG CAGCCGTCGG CGAGAGACTT CGAAGTTCGA 
AGCCTTGTGG GCTGTGGTCA CTTCGCTGAA GTGCAGGTGG TTAGAGAGAA GGCGACCGGG 
GACGTCTATG CCATGAAAAT CATGAAGAAG AAGGCTTTGC TGGCCCAGGA ACAGGTTTCA 
TTTTTCGAGG AGGAGAGGAA CATATTATCT CGGAGCACGA GTCCTTGGAT CCCCCAGTTA 
CAGTACGCCT TTCAGGACAA AAATAACCTT TACCTGGTCA TGGAATATCA GCCTGGAGGG 
GATTTGCTGT CGCTTCTGAA CAGATACGAG GACCAATTAG ATGAGAGCAT GATCCAGTTT 
TACCTTGCTG AGCTGATTTT GGCTGTCCAC AGCGTGCACC AGATGGGATA TGTGCATCGA 
GACATCAAGC CCGAGAACAT CCTCATCGAC CGGACGGGAC ACATCAAGCT GGTGGATTTT 
GGATCAGCCG CTAAGATGAA TTCAAATAAG GTGGATGCCA AACTCCCCAT TGGGACCCCG 
GATTACATGG CTCCGGAAGT GTTGACCGTG ATGAACGAGG ACCGAAGGGG CACATACGGC 
TTGGACTGTG ACTGGTGGTC TGTCGGAGTT GTTGCTTATG AGATGGTTTA TGGGAAGACC 
CCATTCACAG AGGGAACCTC CGCCCGGACC TTCAACAACA TCATGAACTT CCAGCGGTTT 
TTGAAGTTCC CAGATGACCC CAAAGTTAGC AGTGAGCTCC TTGATCTGCT TCAGAGTCTG 
CTGTGTGTCC AGAAAGAGAG ACTGAAGTTC GAGGGTCTCT GCTGCCACCC TTTCTTTGCC 
AGAACGGACT GGAACAACAT CCGTAACTCT CCTCCCCCCT TCGTCCCCAC CCTCAAGTCT 
GACGATGACA CCTCCAATTT TGATGAACCA GAGAAGAATT CGTGGGTTTC ATCCTCTGTG 
TGCCAGCTGA GCCCCTCGGG CTTCTCAGGC GAAGAGCTGC CGTTTGTGGG ATTTTCGTAC 
AGCAAGGCAC TGGGGTATCT TGGTAGATCT GAGTCTGTCG TGTCGAGTCT GGACTCCCCT 
GCCAAGGTTA GCTCCATGGA AAAGAAACTT CTCATCAAAA GCAAAGAGCT CCAAGACTCC 
CAGGACAAGT GTCACAAGAT GGAGCAGGAA ATGACCCGGT TACATCGCAG AGTGTCAGAG 
GTGGAGGCTG TGCTTAGTCA GAAGGAGGTG GAGCTGAAGG CCTCTGAGAC TCAGAGATCC 
CTCCTGGAGC AGGACCTTGC TACCTACATC ACAGAATGCA GTAGCTTAAA GCGAAGTTTG 
GAGCAAGCGC GGATGGAGGT GTCCCAGGAG GATGACAAAG CTCTGCAGCT TCTCCACGAC 
ATCCGAGAGC AGAGCCGGAA GCTCCAGGAG ATCAAGGAGC AGGAGTACCA GGCTCAGGTG 
GAGGAGATGA GGCTGATGAT GAATCAGCTG GAAGAAGACC TGGTGTCAGC CCGCAGACGC 
AGCGATCTCT ACGAGTCTGA GCTGAGGGAG TCTCGGCTTG CCGCCGAGGA ATTCAAGCGG 
AAGGCAAACG AATGTCAGCA CAAACTGATG AAGGTAGTCA GCCACCCTCC AAGAGGGGAC 
TCCGGGGGCA CGGCCCTGGA TGATCTTCAC AAGATGCAAG GCCACGCTGG CTTAACGAGT 
GCTAAGGACC AAGGGAAGCC TGAAGTGGGA GAATATTCCA AACTGGAGAA GATCAATGCT 
GAGCAGCAGC TGAAGATCCA GGAGCTCCAG GAGAAGCTGG AGAAGGCTGT AAAAGCCAGC 
ACAGAGGCCA CCGAGCTCCT GCAGAATATC CGCCAGGCAA AGGAGCGAGC AGAGCGGGAG 
CTAGAGAAGC TACACAACCG GGAAGACTCC TCCGAGGGCA TCAAAAAGAA GCTGGTGGAA 
GCCGAGGAAC GCCGCCACTC CCTGGAGAAC AAGGTAAAGA GACTAGAGAC CATGGAGCGT 
AGAGAGAACA GACTGAAGGA TGACATCCAG ACAAAGTCCG AACAGATCCA GCAGATGGCT 
GATAAAATTC TGGAGCTGGA GGAGAAACAT CGGGAGGCTC AGGTCTCAGC TCAACATCTA 
GAAGTACACT TGAAACAGAA AGAACAGCAC TACGAGGAAA AGATCAAGGT ATTGGACAAT 
CAGATAAAGA AGGACCTGGC CGACAAGGAG AGCCTGGAGA ACATGATGCA GAGACACGAG 
GAGGAGGCCC ACGAGAAGGG CAAGATCCTC AGCGAGCAGA AGGCGATGAT CAACGCGATG 
GATTCCAAGA TCCGATCCCT GGAGCAGAGG ATCGTGGAGC TGTCGGAAGC CAACAAGCTT 
GCGGCAAACA GCAGTCTCTT CACCCAGAGG AACATGAAGG CCCAGGAAGA GATGATCTCA 
GAACTCAGGC AGCAGAAATT TTACCTGGAG ACGCAGGCCG GAAAGCTGGA GGCCCAGAAC 
CGAAAGCTGG AAGAGCAACT GGAGAAGATC AGCCACCAAG ATCACAGTGA CAAGAGTCGG 
CTGCTGGAGC TGGAAACAAG GCTGAGGGAG GTCAGCCTGG AGCACGAGGA GCAGAAGCTG 
GAGCTGAAGC GGCAGCTCAC GGAGCTGCAG CTGTCCCTGC AGGAGCGCGA GTCCCAGCTG 
ACGGCCCTGC AGGCCGCCCG GGCAGCGCTG GAGAGCCAGC TCCGCCAGGC GAAGACAGAG 
CTGGAGGAGA CAACCGCGGA AGCGGAGGAG GAGATCCAGG CGCTCACGGC ACATCGAGAT 
GAGATCCAGC GCAAATTCGA TGCCCTTCGC AACAGCTGCA CGGTCATCAC CGACCTGGAG 
GAGCAGCTGA ACCAGCTCAC CGAGGACAAC GCCGAGCTCA ACAACCAAAA CTTCTACCTG 
TCCAAACAAC TCGATGAGGC TTCCGGGGCC AATGACGAGA TTGTGCAGCT GCGGAGTGAG 
GTGGACCATC TCCGCCGTGA GATCACGGAG CGGGAGATGC AGCTCACCAG CCAGAAGCAA 
GCACAACTCA GTGCTCCTGA TTTGCAGACG ATGGAGGCTC TGAAGACGAC ATGCACCATG 
CTGGAGGAGC AGGTCCTGGA CCTGGAGGCC CTGAACGACG AGCTGCTGGA GAAGGAGCGC 
CAGTGGGAGG CCTGGCGGAG CGTCCTCGGC GACGAGAAGT CCCAGTTCGA GTGTCGAGTT 
CGAGAGCTAC AGAGGATGCT GGACACCGAG AAGCAGAGCA GGGCTAGGGC CGATCAGCGG 
ATCACCGAGT CGCGCCAGGT GGTGGAGTTG GCGGTGAAGG AACACAAGGC CGAGATTCTT 
GCTCTGCAGC AGGCTCTCAA GGAGCAGAAG CTCAAAGCCG AGAGCCTGTC GGACAAGCTC 
AACGACCTGG AGAAGAAGCA CGCCATGCTG GAGATGAACG CCCGGAGCTT ACAGCAGAAA 
CTAGAGACAG AGCGGGAGCT CAAACAGAGG CTTCTGGAGG AGCAAGCCAA ATTGCAGCAG 
CAGATGGACC TGCAGAAGAA CCACATCTTC AGACTGACGC AAGGGCTGCA GGAGGCGCTG 
GACCGGGCGG ATCTGCTGAA GACAGAAAGG AGCGACCTGG AGTACCAGCT GGAAAACATT 
CAGGTTCTCT ACTCTCACGA GAAAGTGAAA ATGGAAGGCA CAATCTCTCA GCAAACCAAA 
CTCATTGATT TCCTGCAAGC CAAAATGGAC CAGCCTGCTA AAAAGAAAAA GGGTTTATTT 
AGTCGACGGA AAGAGGACCC TGCTTTGCCC ACACAGGTTC CTCTGCAGTA CAATGAGCTG 
AAGCTAGCCC TGGAAAAGGA GAAAGCCCGA TGCGCGGAGC TGGAGGAGGC CCTTCAGAAG 
ACCCGCATCG AACTCCGCTC TGCGCGGGAG GAAGCTGCCC ACCGCAAAGC CACGGACCAC 
CCGCACCCGT CTACGCCAGC CACTGCGAGG CAGCAGATCG CCATGTCTGC CATTGTGCGG 
TCGCCCGAGC ACCAGCCCAG TGCCATGAGC CTGCTTGCCC CACCATCCAG CCGCAGAAAG 
GAGTCGTCAA CTCCAGAGGA ATTCAGCCGG CGTCTGAAAG AGCGCATGCA CCACAACATC 
CCTCACCGGT TTAATGTGGG CCTGAACATG AGAGCCACCA AGTGCGCCGT GTGTCTGGAT 
ACTGTGCACT TTGGACGCCA GGCATCCAAA TGCCTCGAAT GTCAAGTGAT GTGTCACCCC 
AAATGCTCCA CCTGCTTGCC CGCTACCTGT GGCCTGCCAG CTGAATATGC CACACACTTC 
ACTGAGGCCT TCTGCCGGGA CAAAATGAAC TCCCCGGGGC TCCAGAGCAA GGAGCCTGGC 
AGCAGCTTGC ACCTGGAAGG GTGGATGAAA GTGCCCAGAA ATAACAAACG GGGACAGCAA 
GGCTGGGACA GGAAGTACAT TGTTCTGGAG GGGTCAAAAG TCCTCATCTA TGACAATGAA 
GCCAGAGAAG CTGGACAGAG GCCGGTGGAA GAATTTGAGC TGTGCCTTCC TGACGGGGAC 
GTATCTATTC ATGGTGCCGT TGGTGCTTCA GAACTTGCAA ATACCGCCAA AGCAGATGTC 
CCATACATCC TGAAGATGGA GTCTCACCCA CACACCACCT GCTGGCCTGG GAGAACCCTC 
TACTTGCTAG CACCCAGCTT CCCCGACAAG CAGCGCTGGG TCACCGCCTT AGAATCTGTC 
GTCGCAGGTG GGAGAGTTTC TAGGGAAAAG GCCGAAGCCG ATGCTAAATT ACTTGGAAAC 
TCTCTGCTGA AACTGGAGGG CGATGACCGG CTTGACATGA ACTGCACCCT GCCCTTCAGT 
GACCAGGTAG TGCTGGTGGG CACGGAGGAA GGCCTCTACG CCCTGAATGT CTTGAAAAAC 
TCCTTAACCC ACATCCCAGG GATTGGCGCA GTCTTCCAAA TTTACATCAT CAAGGACCTG 
GAGAAGCTGC TCATGATAGC AGGGGAAGAG CGGGCTCTGT GCCTGGTGGA CGTGAAGAAG 
GTGAAGCAGT CCCTGGCGCA GTCACACCTG CCTGCCCAGC CCGACGTCTC CCCCAACATA 
TTCGAAGCCG TCAAAGGCTG CCACTTGTTC GCTGCTGGCA AGATCGAGAA CAGCCTGTGC 
ATCTGCGCCG CTATGCCAAG CAAAGTCGTC ATCCTCCGCT ACAATGACAA CCTCAGCAAG 
TACTGCATCC GCAAGGAGAT CGAGACCTCA GAGCCCTGCA GCTGTATCCA CTTCACCAAC 
TACAGCATCC TCATTGGGAC CAACAAATTC TATGAGATCG ACATGAAGCA GTACACGCTT 
GACGAGTTCC TGGACAAGAA CGACCATTCC TTGGCACCTG CTGTGTTCGC CTCCTCGTCC 
AACAGCTTCC CTGTCTCCAT TGTGCAGGCG AACAGCGCCG GGCAGCGAGA AGAATACCTG 
CTGTGCTTCC ACGAATTTGG GGTGTTCGTG GATTCTTACG GAAGACGTAG CCGCACAGAT 
GATCTCAAGT GGAGTCGCTT ACCTCTGGCC TTCGCCTACA GAGAACCTTA TCTGTTTGTG 
ACTCACTTCA ACTCCCTGGA AGTCATTGAG ATCCAGGCCA GATCCTCACT GGGGAGCCCT 
GCCCGAGCAT ATCTGGAAAT TCCAAACCCT CGCTACCTGG GCCCCGCGAT TTCCTCCGGA 
GCGATTTACC TGGCCTCCTC ATACCAGGAC AAGTTAAGGG TCATATGCTG CAAAGGAAAC 
CTCGTGAAGG AGTCAGGCAC TGAGCAGCAC CGGGTGCCCT CCACCTCCCG CAGCAGCCCC 
AACAAGCGAG GCCCACCAAC ATACAACGAG CACATCACCA AACGCGTGGC CTCCAGCCCG 
GCGCCACCGG AAGGCCCCAG CCACCCCCGA GAGCCAAGCA CACCGCACCG CTACCGAGAC 
AGAGAGGGTC GGACAGAGCT GCGCAGGGAC AAGTCTCCAG GCCGCCCTCT GGAGCGGGAG 
AAGTCCCCAG GCCGAATGCT CAGCACTAGG AGAGAGCGGT CCCCAGGGAG ACTGTTTGAA 
GACAGCAGCA GGGGCCGGCT GCCTGCAGGA GCAGTGAGGA CCCCACTGTC CCAGGTTAAC 
AAGGTCTGGG ACCAGTCTTC AGTATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cit ( XM_030254099.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030109959.1 Mus musculus citron (Cit), transcript variant X11, mRNA.

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CloneID	OMu35779
Clone ID Related Accession (Same CDS sequence)	XM_030254099.1 , XM_006530138.3 , XM_006530138.4
Accession Version	XM_030254099.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6285bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	citron Rho-interacting kinase isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000071.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTGAAGT TCAAGTATGG TGTGCGGAAC CCGCCGGAGG CCAGTGCCTC CGAGCCCATT 
GCCAGTCGGG CCTCCAGGCT AAATCTCTTC TTCCAGGGGA AACCGCCCCT CATGACTCAA 
CAGCAGATGT CTGCTCTTTC CCGGGAAGGG ATGCTAGACG CCCTCTTCGC TCTCTTTGAA 
GAGTGCAGCC AACCCGCCCT GATGAAGATG AAGCACGTGA GCAGCTTTGT CCAGAAGTAT 
TCCGACACCA TAGCCGAGTT GCGGGAGCTG CAGCCGTCGG CGAGAGACTT CGAAGTTCGA 
AGCCTTGTGG GCTGTGGTCA CTTCGCTGAA GTGCAGGTGG TTAGAGAGAA GGCGACCGGG 
GACGTCTATG CCATGAAAAT CATGAAGAAG AAGGCTTTGC TGGCCCAGGA ACAGGTTTCA 
TTTTTCGAGG AGGAGAGGAA CATATTATCT CGGAGCACGA GTCCTTGGAT CCCCCAGTTA 
CAGTACGCCT TTCAGGACAA AAATAACCTT TACCTGGTCA TGGAATATCA GCCTGGAGGG 
GATTTGCTGT CGCTTCTGAA CAGATACGAG GACCAATTAG ATGAGAGCAT GATCCAGTTT 
TACCTTGCTG AGCTGATTTT GGCTGTCCAC AGCGTGCACC AGATGGGATA TGTGCATCGA 
GACATCAAGC CCGAGAACAT CCTCATCGAC CGGACGGGAC ACATCAAGCT GGTGGATTTT 
GGATCAGCCG CTAAGATGAA TTCAAATAAG GTGGATGCCA AACTCCCCAT TGGGACCCCG 
GATTACATGG CTCCGGAAGT GTTGACCGTG ATGAACGAGG ACCGAAGGGG CACATACGGC 
TTGGACTGTG ACTGGTGGTC TGTCGGAGTT GTTGCTTATG AGATGGTTTA TGGGAAGACC 
CCATTCACAG AGGGAACCTC CGCCCGGACC TTCAACAACA TCATGAACTT CCAGCGGTTT 
TTGAAGTTCC CAGATGACCC CAAAGTTAGC AGTGAGCTCC TTGATCTGCT TCAGAGTCTG 
CTGTGTGTCC AGAAAGAGAG ACTGAAGTTC GAGGGTCTCT GCTGCCACCC TTTCTTTGCC 
AGAACGGACT GGAACAACAT CCGTAACTCT CCTCCCCCCT TCGTCCCCAC CCTCAAGTCT 
GACGATGACA CCTCCAATTT TGATGAACCA GAGAAGAATT CGTGGGTTTC ATCCTCTGTG 
TGCCAGCTGA GCCCCTCGGG CTTCTCAGGC GAAGAGCTGC CGTTTGTGGG ATTTTCGTAC 
AGCAAGGCAC TGGGGTATCT TGGTAGATCT GAGTCTGTCG TGTCGAGTCT GGACTCCCCT 
GCCAAGGTTA GCTCCATGGA AAAGAAACTT CTCATCAAAA GCAAAGAGCT CCAAGACTCC 
CAGGACAAGT GTCACAAGAT GGAGCAGGAA ATGACCCGGT TACATCGCAG AGTGTCAGAG 
GTGGAGGCTG TGCTTAGTCA GAAGGAGGTG GAGCTGAAGG CCTCTGAGAC TCAGAGATCC 
CTCCTGGAGC AGGACCTTGC TACCTACATC ACAGAATGCA GTAGCTTAAA GCGAAGTTTG 
GAGCAAGCGC GGATGGAGGT GTCCCAGGAG GATGACAAAG CTCTGCAGCT TCTCCACGAC 
ATCCGAGAGC AGAGCCGGAA GCTCCAGGAG ATCAAGGAGC AGGAGTACCA GGCTCAGGTG 
GAGGAGATGA GGCTGATGAT GAATCAGCTG GAAGAAGACC TGGTGTCAGC CCGCAGACGC 
AGCGATCTCT ACGAGTCTGA GCTGAGGGAG TCTCGGCTTG CCGCCGAGGA ATTCAAGCGG 
AAGGCAAACG AATGTCAGCA CAAACTGATG AAGGCTAAGG ACCAAGGGAA GCCTGAAGTG 
GGAGAATATT CCAAACTGGA GAAGATCAAT GCTGAGCAGC AGCTGAAGAT CCAGGAGCTC 
CAGGAGAAGC TGGAGAAGGC TGTAAAAGCC AGCACAGAGG CCACCGAGCT CCTGCAGAAT 
ATCCGCCAGG CAAAGGAGCG AGCAGAGCGG GAGCTAGAGA AGCTACACAA CCGGGAAGAC 
TCCTCCGAGG GCATCAAAAA GAAGCTGGTG GAAGCCGAGG AACGCCGCCA CTCCCTGGAG 
AACAAGGTAA AGAGACTAGA GACCATGGAG CGTAGAGAGA ACAGACTGAA GGATGACATC 
CAGACAAAGT CCGAACAGAT CCAGCAGATG GCTGATAAAA TTCTGGAGCT GGAGGAGAAA 
CATCGGGAGG CTCAGGTCTC AGCTCAACAT CTAGAAGTAC ACTTGAAACA GAAAGAACAG 
CACTACGAGG AAAAGATCAA GGTATTGGAC AATCAGATAA AGAAGGACCT GGCCGACAAG 
GAGAGCCTGG AGAACATGAT GCAGAGACAC GAGGAGGAGG CCCACGAGAA GGGCAAGATC 
CTCAGCGAGC AGAAGGCGAT GATCAACGCG ATGGATTCCA AGATCCGATC CCTGGAGCAG 
AGGATCGTGG AGCTGTCGGA AGCCAACAAG CTTGCGGCAA ACAGCAGTCT CTTCACCCAG 
AGGAACATGA AGGCCCAGGA AGAGATGATC TCAGAACTCA GGCAGCAGAA ATTTTACCTG 
GAGACGCAGG CCGGAAAGCT GGAGGCCCAG AACCGAAAGC TGGAAGAGCA ACTGGAGAAG 
ATCAGCCACC AAGATCACAG TGACAAGAGT CGGCTGCTGG AGCTGGAAAC AAGGCTGAGG 
GAGGTCAGCC TGGAGCACGA GGAGCAGAAG CTGGAGCTGA AGCGGCAGCT CACGGAGCTG 
CAGCTGTCCC TGCAGGAGCG CGAGTCCCAG CTGACGGCCC TGCAGGCCGC CCGGGCAGCG 
CTGGAGAGCC AGCTCCGCCA GGCGAAGACA GAGCTGGAGG AGACAACCGC GGAAGCGGAG 
GAGGAGATCC AGGCGCTCAC GGCACATCGA GATGAGATCC AGCGCAAATT CGATGCCCTT 
CGCAACAGCT GCACGGTCAT CACCGACCTG GAGGAGCAGC TGAACCAGCT CACCGAGGAC 
AACGCCGAGC TCAACAACCA AAACTTCTAC CTGTCCAAAC AACTCGATGA GGCTTCCGGG 
GCCAATGACG AGATTGTGCA GCTGCGGAGT GAGGTGGACC ATCTCCGCCG TGAGATCACG 
GAGCGGGAGA TGCAGCTCAC CAGCCAGAAG CAAGCACAAC TCAGTGCTCC TGATTTGCAG 
ACGATGGAGG CTCTGAAGAC GACATGCACC ATGCTGGAGG AGCAGGTCCT GGACCTGGAG 
GCCCTGAACG ACGAGCTGCT GGAGAAGGAG CGCCAGTGGG AGGCCTGGCG GAGCGTCCTC 
GGCGACGAGA AGTCCCAGTT CGAGTGTCGA GTTCGAGAGC TACAGAGGAT GCTGGACACC 
GAGAAGCAGA GCAGGGCTAG GGCCGATCAG CGGATCACCG AGTCGCGCCA GGTGGTGGAG 
TTGGCGGTGA AGGAACACAA GGCCGAGATT CTTGCTCTGC AGCAGGCTCT CAAGGAGCAG 
AAGCTCAAAG CCGAGAGCCT GTCGGACAAG CTCAACGACC TGGAGAAGAA GCACGCCATG 
CTGGAGATGA ACGCCCGGAG CTTACAGCAG AAACTAGAGA CAGAGCGGGA GCTCAAACAG 
AGGCTTCTGG AGGAGCAAGC CAAATTGCAG CAGCAGATGG ACCTGCAGAA GAACCACATC 
TTCAGACTGA CGCAAGGGCT GCAGGAGGCG CTGGACCGGG CGGATCTGCT GAAGACAGAA 
AGGAGCGACC TGGAGTACCA GCTGGAAAAC ATTCAGGTTC TCTACTCTCA CGAGAAAGTG 
AAAATGGAAG GCACAATCTC TCAGCAAACC AAACTCATTG ATTTCCTGCA AGCCAAAATG 
GACCAGCCTG CTAAAAAGAA AAAGGGTTTA TTTAGTCGAC GGAAAGAGGA CCCTGCTTTG 
CCCACACAGG TTCCTCTGCA GTACAATGAG CTGAAGCTAG CCCTGGAAAA GGAGAAAGCC 
CGATGCGCGG AGCTGGAGGA GGCCCTTCAG AAGACCCGCA TCGAACTCCG CTCTGCGCGG 
GAGGAAGCTG CCCACCGCAA AGCCACGGAC CACCCGCACC CGTCTACGCC AGCCACTGCG 
AGGCAGCAGA TCGCCATGTC TGCCATTGTG CGGTCGCCCG AGCACCAGCC CAGTGCCATG 
AGCCTGCTTG CCCCACCATC CAGCCGCAGA AAGGAGTCGT CAACTCCAGA GGAATTCAGC 
CGGCGTCTGA AAGAGCGCAT GCACCACAAC ATCCCTCACC GGTTTAATGT GGGCCTGAAC 
ATGAGAGCCA CCAAGTGCGC CGTGTGTCTG GATACTGTGC ACTTTGGACG CCAGGCATCC 
AAATGCCTCG AATGTCAAGT GATGTGTCAC CCCAAATGCT CCACCTGCTT GCCCGCTACC 
TGTGGCCTGC CAGCTGAATA TGCCACACAC TTCACTGAGG CCTTCTGCCG GGACAAAATG 
AACTCCCCGG GGCTCCAGAG CAAGGAGCCT GGCAGCAGCT TGCACCTGGA AGGGTGGATG 
AAAGTGCCCA GAAATAACAA ACGGGGACAG CAAGGCTGGG ACAGGAAGTA CATTGTTCTG 
GAGGGGTCAA AAGTCCTCAT CTATGACAAT GAAGCCAGAG AAGCTGGACA GAGGCCGGTG 
GAAGAATTTG AGCTGTGCCT TCCTGACGGG GACGTATCTA TTCATGGTGC CGTTGGTGCT 
TCAGAACTTG CAAATACCGC CAAAGCAGAT GTCCCATACA TCCTGAAGAT GGAGTCTCAC 
CCACACACCA CCTGCTGGCC TGGGAGAACC CTCTACTTGC TAGCACCCAG CTTCCCCGAC 
AAGCAGCGCT GGGTCACCGC CTTAGAATCT GTCGTCGCAG GTGGGAGAGT TTCTAGGGAA 
AAGGCCGAAG CCGATGCTGC ACGTGACCAT ACTTCGAGTG AGCATCAGCC AGTGTGGGTT 
GAGAAATTAC TTGGAAACTC TCTGCTGAAA CTGGAGGGCG ATGACCGGCT TGACATGAAC 
TGCACCCTGC CCTTCAGTGA CCAGGTAGTG CTGGTGGGCA CGGAGGAAGG CCTCTACGCC 
CTGAATGTCT TGAAAAACTC CTTAACCCAC ATCCCAGGGA TTGGCGCAGT CTTCCAAATT 
TACATCATCA AGGACCTGGA GAAGCTGCTC ATGATAGCAG GGGAAGAGCG GGCTCTGTGC 
CTGGTGGACG TGAAGAAGGT GAAGCAGTCC CTGGCGCAGT CACACCTGCC TGCCCAGCCC 
GACGTCTCCC CCAACATATT CGAAGCCGTC AAAGGCTGCC ACTTGTTCGC TGCTGGCAAG 
ATCGAGAACA GCCTGTGCAT CTGCGCCGCT ATGCCAAGCA AAGTCGTCAT CCTCCGCTAC 
AATGACAACC TCAGCAAGTA CTGCATCCGC AAGGAGATCG AGACCTCAGA GCCCTGCAGC 
TGTATCCACT TCACCAACTA CAGCATCCTC ATTGGGACCA ACAAATTCTA TGAGATCGAC 
ATGAAGCAGT ACACGCTTGA CGAGTTCCTG GACAAGAACG ACCATTCCTT GGCACCTGCT 
GTGTTCGCCT CCTCGTCCAA CAGCTTCCCT GTCTCCATTG TGCAGGCGAA CAGCGCCGGG 
CAGCGAGAAG AATACCTGCT GTGCTTCCAC GAATTTGGGG TGTTCGTGGA TTCTTACGGA 
AGACGTAGCC GCACAGATGA TCTCAAGTGG AGTCGCTTAC CTCTGGCCTT CGCCTACAGA 
GAACCTTATC TGTTTGTGAC TCACTTCAAC TCCCTGGAAG TCATTGAGAT CCAGGCCAGA 
TCCTCACTGG GGAGCCCTGC CCGAGCATAT CTGGAAATTC CAAACCCTCG CTACCTGGGC 
CCCGCGATTT CCTCCGGAGC GATTTACCTG GCCTCCTCAT ACCAGGACAA GTTAAGGGTC 
ATATGCTGCA AAGGAAACCT CGTGAAGGAG TCAGGCACTG AGCAGCACCG GGTGCCCTCC 
ACCTCCCGCA GCAGCCCCAA CAAGCGAGGC CCACCAACAT ACAACGAGCA CATCACCAAA 
CGCGTGGCCT CCAGCCCGGC GCCACCGGAA GGCCCCAGCC ACCCCCGAGA GCCAAGCACA 
CCGCACCGCT ACCGAGACAG AGAGGGTCGG ACAGAGCTGC GCAGGGACAA GTCTCCAGGC 
CGCCCTCTGG AGCGGGAGAA GTCCCCAGGC CGAATGCTCA GCACTAGGAG AGAGCGGTCC 
CCAGGGAGAC TGTTTGAAGA CAGCAGCAGG GGCCGGCTGC CTGCAGGAGC AGTGAGGACC 
CCACTGTCCC AGGTTAACAA GGTCTGGGAC CAGTCTTCAG TATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030109959.1
CDS	103..6387
Translation	MLKFKYGVRNPPEASASEPIASRASRLNLFFQGKPPLMTQQQMSALSREGMLDALFALFEECSQPALMKMKHVSSFVQKYSDTIAELRELQPSARDFEVRSLVGCGHFAEVQVVREKATGDVYAMKIMKKKALLAQEQVSFFEEERNILSRSTSPWIPQLQYAFQDKNNLYLVMEYQPGGDLLSLLNRYEDQLDESMIQFYLAELILAVHSVHQMGYVHRDIKPENILIDRTGHIKLVDFGSAAKMNSNKVDAKLPIGTPDYMAPEVLTVMNEDRRGTYGLDCDWWSVGVVAYEMVYGKTPFTEGTSARTFNNIMNFQRFLKFPDDPKVSSELLDLLQSLLCVQKERLKFEGLCCHPFFARTDWNNIRNSPPPFVPTLKSDDDTSNFDEPEKNSWVSSSVCQLSPSGFSGEELPFVGFSYSKALGYLGRSESVVSSLDSPAKVSSMEKKLLIKSKELQDSQDKCHKMEQEMTRLHRRVSEVEAVLSQKEVELKASETQRSLLEQDLATYITECSSLKRSLEQARMEVSQEDDKALQLLHDIREQSRKLQEIKEQEYQAQVEEMRLMMNQLEEDLVSARRRSDLYESELRESRLAAEEFKRKANECQHKLMKAKDQGKPEVGEYSKLEKINAEQQLKIQELQEKLEKAVKASTEATELLQNIRQAKERAERELEKLHNREDSSEGIKKKLVEAEERRHSLENKVKRLETMERRENRLKDDIQTKSEQIQQMADKILELEEKHREAQVSAQHLEVHLKQKEQHYEEKIKVLDNQIKKDLADKESLENMMQRHEEEAHEKGKILSEQKAMINAMDSKIRSLEQRIVELSEANKLAANSSLFTQRNMKAQEEMISELRQQKFYLETQAGKLEAQNRKLEEQLEKISHQDHSDKSRLLELETRLREVSLEHEEQKLELKRQLTELQLSLQERESQLTALQAARAALESQLRQAKTELEETTAEAEEEIQALTAHRDEIQRKFDALRNSCTVITDLEEQLNQLTEDNAELNNQNFYLSKQLDEASGANDEIVQLRSEVDHLRREITEREMQLTSQKQAQLSAPDLQTMEALKTTCTMLEEQVLDLEALNDELLEKERQWEAWRSVLGDEKSQFECRVRELQRMLDTEKQSRARADQRITESRQVVELAVKEHKAEILALQQALKEQKLKAESLSDKLNDLEKKHAMLEMNARSLQQKLETERELKQRLLEEQAKLQQQMDLQKNHIFRLTQGLQEALDRADLLKTERSDLEYQLENIQVLYSHEKVKMEGTISQQTKLIDFLQAKMDQPAKKKKGLFSRRKEDPALPTQVPLQYNELKLALEKEKARCAELEEALQKTRIELRSAREEAAHRKATDHPHPSTPATARQQIAMSAIVRSPEHQPSAMSLLAPPSSRRKESSTPEEFSRRLKERMHHNIPHRFNVGLNMRATKCAVCLDTVHFGRQASKCLECQVMCHPKCSTCLPATCGLPAEYATHFTEAFCRDKMNSPGLQSKEPGSSLHLEGWMKVPRNNKRGQQGWDRKYIVLEGSKVLIYDNEAREAGQRPVEEFELCLPDGDVSIHGAVGASELANTAKADVPYILKMESHPHTTCWPGRTLYLLAPSFPDKQRWVTALESVVAGGRVSREKAEADAARDHTSSEHQPVWVEKLLGNSLLKLEGDDRLDMNCTLPFSDQVVLVGTEEGLYALNVLKNSLTHIPGIGAVFQIYIIKDLEKLLMIAGEERALCLVDVKKVKQSLAQSHLPAQPDVSPNIFEAVKGCHLFAAGKIENSLCICAAMPSKVVILRYNDNLSKYCIRKEIETSEPCSCIHFTNYSILIGTNKFYEIDMKQYTLDEFLDKNDHSLAPAVFASSSNSFPVSIVQANSAGQREEYLLCFHEFGVFVDSYGRRSRTDDLKWSRLPLAFAYREPYLFVTHFNSLEVIEIQARSSLGSPARAYLEIPNPRYLGPAISSGAIYLASSYQDKLRVICCKGNLVKESGTEQHRVPSTSRSSPNKRGPPTYNEHITKRVASSPAPPEGPSHPREPSTPHRYRDREGRTELRRDKSPGRPLEREKSPGRMLSTRRERSPGRLFEDSSRGRLPAGAVRTPLSQVNKVWDQSSV

Target ORF information:

RefSeq Version	XM_030254099.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus citron (Cit), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030254099.1

ORF Insert Sequence:

ATGTTGAAGT TCAAGTATGG TGTGCGGAAC CCGCCGGAGG CCAGTGCCTC CGAGCCCATT 
GCCAGTCGGG CCTCCAGGCT AAATCTCTTC TTCCAGGGGA AACCGCCCCT CATGACTCAA 
CAGCAGATGT CTGCTCTTTC CCGGGAAGGG ATGCTAGACG CCCTCTTCGC TCTCTTTGAA 
GAGTGCAGCC AACCCGCCCT GATGAAGATG AAGCACGTGA GCAGCTTTGT CCAGAAGTAT 
TCCGACACCA TAGCCGAGTT GCGGGAGCTG CAGCCGTCGG CGAGAGACTT CGAAGTTCGA 
AGCCTTGTGG GCTGTGGTCA CTTCGCTGAA GTGCAGGTGG TTAGAGAGAA GGCGACCGGG 
GACGTCTATG CCATGAAAAT CATGAAGAAG AAGGCTTTGC TGGCCCAGGA ACAGGTTTCA 
TTTTTCGAGG AGGAGAGGAA CATATTATCT CGGAGCACGA GTCCTTGGAT CCCCCAGTTA 
CAGTACGCCT TTCAGGACAA AAATAACCTT TACCTGGTCA TGGAATATCA GCCTGGAGGG 
GATTTGCTGT CGCTTCTGAA CAGATACGAG GACCAATTAG ATGAGAGCAT GATCCAGTTT 
TACCTTGCTG AGCTGATTTT GGCTGTCCAC AGCGTGCACC AGATGGGATA TGTGCATCGA 
GACATCAAGC CCGAGAACAT CCTCATCGAC CGGACGGGAC ACATCAAGCT GGTGGATTTT 
GGATCAGCCG CTAAGATGAA TTCAAATAAG GTGGATGCCA AACTCCCCAT TGGGACCCCG 
GATTACATGG CTCCGGAAGT GTTGACCGTG ATGAACGAGG ACCGAAGGGG CACATACGGC 
TTGGACTGTG ACTGGTGGTC TGTCGGAGTT GTTGCTTATG AGATGGTTTA TGGGAAGACC 
CCATTCACAG AGGGAACCTC CGCCCGGACC TTCAACAACA TCATGAACTT CCAGCGGTTT 
TTGAAGTTCC CAGATGACCC CAAAGTTAGC AGTGAGCTCC TTGATCTGCT TCAGAGTCTG 
CTGTGTGTCC AGAAAGAGAG ACTGAAGTTC GAGGGTCTCT GCTGCCACCC TTTCTTTGCC 
AGAACGGACT GGAACAACAT CCGTAACTCT CCTCCCCCCT TCGTCCCCAC CCTCAAGTCT 
GACGATGACA CCTCCAATTT TGATGAACCA GAGAAGAATT CGTGGGTTTC ATCCTCTGTG 
TGCCAGCTGA GCCCCTCGGG CTTCTCAGGC GAAGAGCTGC CGTTTGTGGG ATTTTCGTAC 
AGCAAGGCAC TGGGGTATCT TGGTAGATCT GAGTCTGTCG TGTCGAGTCT GGACTCCCCT 
GCCAAGGTTA GCTCCATGGA AAAGAAACTT CTCATCAAAA GCAAAGAGCT CCAAGACTCC 
CAGGACAAGT GTCACAAGAT GGAGCAGGAA ATGACCCGGT TACATCGCAG AGTGTCAGAG 
GTGGAGGCTG TGCTTAGTCA GAAGGAGGTG GAGCTGAAGG CCTCTGAGAC TCAGAGATCC 
CTCCTGGAGC AGGACCTTGC TACCTACATC ACAGAATGCA GTAGCTTAAA GCGAAGTTTG 
GAGCAAGCGC GGATGGAGGT GTCCCAGGAG GATGACAAAG CTCTGCAGCT TCTCCACGAC 
ATCCGAGAGC AGAGCCGGAA GCTCCAGGAG ATCAAGGAGC AGGAGTACCA GGCTCAGGTG 
GAGGAGATGA GGCTGATGAT GAATCAGCTG GAAGAAGACC TGGTGTCAGC CCGCAGACGC 
AGCGATCTCT ACGAGTCTGA GCTGAGGGAG TCTCGGCTTG CCGCCGAGGA ATTCAAGCGG 
AAGGCAAACG AATGTCAGCA CAAACTGATG AAGGCTAAGG ACCAAGGGAA GCCTGAAGTG 
GGAGAATATT CCAAACTGGA GAAGATCAAT GCTGAGCAGC AGCTGAAGAT CCAGGAGCTC 
CAGGAGAAGC TGGAGAAGGC TGTAAAAGCC AGCACAGAGG CCACCGAGCT CCTGCAGAAT 
ATCCGCCAGG CAAAGGAGCG AGCAGAGCGG GAGCTAGAGA AGCTACACAA CCGGGAAGAC 
TCCTCCGAGG GCATCAAAAA GAAGCTGGTG GAAGCCGAGG AACGCCGCCA CTCCCTGGAG 
AACAAGGTAA AGAGACTAGA GACCATGGAG CGTAGAGAGA ACAGACTGAA GGATGACATC 
CAGACAAAGT CCGAACAGAT CCAGCAGATG GCTGATAAAA TTCTGGAGCT GGAGGAGAAA 
CATCGGGAGG CTCAGGTCTC AGCTCAACAT CTAGAAGTAC ACTTGAAACA GAAAGAACAG 
CACTACGAGG AAAAGATCAA GGTATTGGAC AATCAGATAA AGAAGGACCT GGCCGACAAG 
GAGAGCCTGG AGAACATGAT GCAGAGACAC GAGGAGGAGG CCCACGAGAA GGGCAAGATC 
CTCAGCGAGC AGAAGGCGAT GATCAACGCG ATGGATTCCA AGATCCGATC CCTGGAGCAG 
AGGATCGTGG AGCTGTCGGA AGCCAACAAG CTTGCGGCAA ACAGCAGTCT CTTCACCCAG 
AGGAACATGA AGGCCCAGGA AGAGATGATC TCAGAACTCA GGCAGCAGAA ATTTTACCTG 
GAGACGCAGG CCGGAAAGCT GGAGGCCCAG AACCGAAAGC TGGAAGAGCA ACTGGAGAAG 
ATCAGCCACC AAGATCACAG TGACAAGAGT CGGCTGCTGG AGCTGGAAAC AAGGCTGAGG 
GAGGTCAGCC TGGAGCACGA GGAGCAGAAG CTGGAGCTGA AGCGGCAGCT CACGGAGCTG 
CAGCTGTCCC TGCAGGAGCG CGAGTCCCAG CTGACGGCCC TGCAGGCCGC CCGGGCAGCG 
CTGGAGAGCC AGCTCCGCCA GGCGAAGACA GAGCTGGAGG AGACAACCGC GGAAGCGGAG 
GAGGAGATCC AGGCGCTCAC GGCACATCGA GATGAGATCC AGCGCAAATT CGATGCCCTT 
CGCAACAGCT GCACGGTCAT CACCGACCTG GAGGAGCAGC TGAACCAGCT CACCGAGGAC 
AACGCCGAGC TCAACAACCA AAACTTCTAC CTGTCCAAAC AACTCGATGA GGCTTCCGGG 
GCCAATGACG AGATTGTGCA GCTGCGGAGT GAGGTGGACC ATCTCCGCCG TGAGATCACG 
GAGCGGGAGA TGCAGCTCAC CAGCCAGAAG CAAGCACAAC TCAGTGCTCC TGATTTGCAG 
ACGATGGAGG CTCTGAAGAC GACATGCACC ATGCTGGAGG AGCAGGTCCT GGACCTGGAG 
GCCCTGAACG ACGAGCTGCT GGAGAAGGAG CGCCAGTGGG AGGCCTGGCG GAGCGTCCTC 
GGCGACGAGA AGTCCCAGTT CGAGTGTCGA GTTCGAGAGC TACAGAGGAT GCTGGACACC 
GAGAAGCAGA GCAGGGCTAG GGCCGATCAG CGGATCACCG AGTCGCGCCA GGTGGTGGAG 
TTGGCGGTGA AGGAACACAA GGCCGAGATT CTTGCTCTGC AGCAGGCTCT CAAGGAGCAG 
AAGCTCAAAG CCGAGAGCCT GTCGGACAAG CTCAACGACC TGGAGAAGAA GCACGCCATG 
CTGGAGATGA ACGCCCGGAG CTTACAGCAG AAACTAGAGA CAGAGCGGGA GCTCAAACAG 
AGGCTTCTGG AGGAGCAAGC CAAATTGCAG CAGCAGATGG ACCTGCAGAA GAACCACATC 
TTCAGACTGA CGCAAGGGCT GCAGGAGGCG CTGGACCGGG CGGATCTGCT GAAGACAGAA 
AGGAGCGACC TGGAGTACCA GCTGGAAAAC ATTCAGGTTC TCTACTCTCA CGAGAAAGTG 
AAAATGGAAG GCACAATCTC TCAGCAAACC AAACTCATTG ATTTCCTGCA AGCCAAAATG 
GACCAGCCTG CTAAAAAGAA AAAGGGTTTA TTTAGTCGAC GGAAAGAGGA CCCTGCTTTG 
CCCACACAGG TTCCTCTGCA GTACAATGAG CTGAAGCTAG CCCTGGAAAA GGAGAAAGCC 
CGATGCGCGG AGCTGGAGGA GGCCCTTCAG AAGACCCGCA TCGAACTCCG CTCTGCGCGG 
GAGGAAGCTG CCCACCGCAA AGCCACGGAC CACCCGCACC CGTCTACGCC AGCCACTGCG 
AGGCAGCAGA TCGCCATGTC TGCCATTGTG CGGTCGCCCG AGCACCAGCC CAGTGCCATG 
AGCCTGCTTG CCCCACCATC CAGCCGCAGA AAGGAGTCGT CAACTCCAGA GGAATTCAGC 
CGGCGTCTGA AAGAGCGCAT GCACCACAAC ATCCCTCACC GGTTTAATGT GGGCCTGAAC 
ATGAGAGCCA CCAAGTGCGC CGTGTGTCTG GATACTGTGC ACTTTGGACG CCAGGCATCC 
AAATGCCTCG AATGTCAAGT GATGTGTCAC CCCAAATGCT CCACCTGCTT GCCCGCTACC 
TGTGGCCTGC CAGCTGAATA TGCCACACAC TTCACTGAGG CCTTCTGCCG GGACAAAATG 
AACTCCCCGG GGCTCCAGAG CAAGGAGCCT GGCAGCAGCT TGCACCTGGA AGGGTGGATG 
AAAGTGCCCA GAAATAACAA ACGGGGACAG CAAGGCTGGG ACAGGAAGTA CATTGTTCTG 
GAGGGGTCAA AAGTCCTCAT CTATGACAAT GAAGCCAGAG AAGCTGGACA GAGGCCGGTG 
GAAGAATTTG AGCTGTGCCT TCCTGACGGG GACGTATCTA TTCATGGTGC CGTTGGTGCT 
TCAGAACTTG CAAATACCGC CAAAGCAGAT GTCCCATACA TCCTGAAGAT GGAGTCTCAC 
CCACACACCA CCTGCTGGCC TGGGAGAACC CTCTACTTGC TAGCACCCAG CTTCCCCGAC 
AAGCAGCGCT GGGTCACCGC CTTAGAATCT GTCGTCGCAG GTGGGAGAGT TTCTAGGGAA 
AAGGCCGAAG CCGATGCTGC ACGTGACCAT ACTTCGAGTG AGCATCAGCC AGTGTGGGTT 
GAGAAATTAC TTGGAAACTC TCTGCTGAAA CTGGAGGGCG ATGACCGGCT TGACATGAAC 
TGCACCCTGC CCTTCAGTGA CCAGGTAGTG CTGGTGGGCA CGGAGGAAGG CCTCTACGCC 
CTGAATGTCT TGAAAAACTC CTTAACCCAC ATCCCAGGGA TTGGCGCAGT CTTCCAAATT 
TACATCATCA AGGACCTGGA GAAGCTGCTC ATGATAGCAG GGGAAGAGCG GGCTCTGTGC 
CTGGTGGACG TGAAGAAGGT GAAGCAGTCC CTGGCGCAGT CACACCTGCC TGCCCAGCCC 
GACGTCTCCC CCAACATATT CGAAGCCGTC AAAGGCTGCC ACTTGTTCGC TGCTGGCAAG 
ATCGAGAACA GCCTGTGCAT CTGCGCCGCT ATGCCAAGCA AAGTCGTCAT CCTCCGCTAC 
AATGACAACC TCAGCAAGTA CTGCATCCGC AAGGAGATCG AGACCTCAGA GCCCTGCAGC 
TGTATCCACT TCACCAACTA CAGCATCCTC ATTGGGACCA ACAAATTCTA TGAGATCGAC 
ATGAAGCAGT ACACGCTTGA CGAGTTCCTG GACAAGAACG ACCATTCCTT GGCACCTGCT 
GTGTTCGCCT CCTCGTCCAA CAGCTTCCCT GTCTCCATTG TGCAGGCGAA CAGCGCCGGG 
CAGCGAGAAG AATACCTGCT GTGCTTCCAC GAATTTGGGG TGTTCGTGGA TTCTTACGGA 
AGACGTAGCC GCACAGATGA TCTCAAGTGG AGTCGCTTAC CTCTGGCCTT CGCCTACAGA 
GAACCTTATC TGTTTGTGAC TCACTTCAAC TCCCTGGAAG TCATTGAGAT CCAGGCCAGA 
TCCTCACTGG GGAGCCCTGC CCGAGCATAT CTGGAAATTC CAAACCCTCG CTACCTGGGC 
CCCGCGATTT CCTCCGGAGC GATTTACCTG GCCTCCTCAT ACCAGGACAA GTTAAGGGTC 
ATATGCTGCA AAGGAAACCT CGTGAAGGAG TCAGGCACTG AGCAGCACCG GGTGCCCTCC 
ACCTCCCGCA GCAGCCCCAA CAAGCGAGGC CCACCAACAT ACAACGAGCA CATCACCAAA 
CGCGTGGCCT CCAGCCCGGC GCCACCGGAA GGCCCCAGCC ACCCCCGAGA GCCAAGCACA 
CCGCACCGCT ACCGAGACAG AGAGGGTCGG ACAGAGCTGC GCAGGGACAA GTCTCCAGGC 
CGCCCTCTGG AGCGGGAGAA GTCCCCAGGC CGAATGCTCA GCACTAGGAG AGAGCGGTCC 
CCAGGGAGAC TGTTTGAAGA CAGCAGCAGG GGCCGGCTGC CTGCAGGAGC AGTGAGGACC 
CCACTGTCCC AGGTTAACAA GGTCTGGGAC CAGTCTTCAG TATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cit ( XM_006530138.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006530201.1 Mus musculus citron (Cit), transcript variant X10, mRNA.

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CloneID	OMu35779
Clone ID Related Accession (Same CDS sequence)	XM_030254099.1 , XM_006530138.3 , XM_006530138.4
Accession Version	XM_006530138.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6285bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	citron Rho-interacting kinase isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000071.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006530138.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTGAAGT TCAAGTATGG TGTGCGGAAC CCGCCGGAGG CCAGTGCCTC CGAGCCCATT 
GCCAGTCGGG CCTCCAGGCT AAATCTCTTC TTCCAGGGGA AACCGCCCCT CATGACTCAA 
CAGCAGATGT CTGCTCTTTC CCGGGAAGGG ATGCTAGACG CCCTCTTCGC TCTCTTTGAA 
GAGTGCAGCC AACCCGCCCT GATGAAGATG AAGCACGTGA GCAGCTTTGT CCAGAAGTAT 
TCCGACACCA TAGCCGAGTT GCGGGAGCTG CAGCCGTCGG CGAGAGACTT CGAAGTTCGA 
AGCCTTGTGG GCTGTGGTCA CTTCGCTGAA GTGCAGGTGG TTAGAGAGAA GGCGACCGGG 
GACGTCTATG CCATGAAAAT CATGAAGAAG AAGGCTTTGC TGGCCCAGGA ACAGGTTTCA 
TTTTTCGAGG AGGAGAGGAA CATATTATCT CGGAGCACGA GTCCTTGGAT CCCCCAGTTA 
CAGTACGCCT TTCAGGACAA AAATAACCTT TACCTGGTCA TGGAATATCA GCCTGGAGGG 
GATTTGCTGT CGCTTCTGAA CAGATACGAG GACCAATTAG ATGAGAGCAT GATCCAGTTT 
TACCTTGCTG AGCTGATTTT GGCTGTCCAC AGCGTGCACC AGATGGGATA TGTGCATCGA 
GACATCAAGC CCGAGAACAT CCTCATCGAC CGGACGGGAC ACATCAAGCT GGTGGATTTT 
GGATCAGCCG CTAAGATGAA TTCAAATAAG GTGGATGCCA AACTCCCCAT TGGGACCCCG 
GATTACATGG CTCCGGAAGT GTTGACCGTG ATGAACGAGG ACCGAAGGGG CACATACGGC 
TTGGACTGTG ACTGGTGGTC TGTCGGAGTT GTTGCTTATG AGATGGTTTA TGGGAAGACC 
CCATTCACAG AGGGAACCTC CGCCCGGACC TTCAACAACA TCATGAACTT CCAGCGGTTT 
TTGAAGTTCC CAGATGACCC CAAAGTTAGC AGTGAGCTCC TTGATCTGCT TCAGAGTCTG 
CTGTGTGTCC AGAAAGAGAG ACTGAAGTTC GAGGGTCTCT GCTGCCACCC TTTCTTTGCC 
AGAACGGACT GGAACAACAT CCGTAACTCT CCTCCCCCCT TCGTCCCCAC CCTCAAGTCT 
GACGATGACA CCTCCAATTT TGATGAACCA GAGAAGAATT CGTGGGTTTC ATCCTCTGTG 
TGCCAGCTGA GCCCCTCGGG CTTCTCAGGC GAAGAGCTGC CGTTTGTGGG ATTTTCGTAC 
AGCAAGGCAC TGGGGTATCT TGGTAGATCT GAGTCTGTCG TGTCGAGTCT GGACTCCCCT 
GCCAAGGTTA GCTCCATGGA AAAGAAACTT CTCATCAAAA GCAAAGAGCT CCAAGACTCC 
CAGGACAAGT GTCACAAGAT GGAGCAGGAA ATGACCCGGT TACATCGCAG AGTGTCAGAG 
GTGGAGGCTG TGCTTAGTCA GAAGGAGGTG GAGCTGAAGG CCTCTGAGAC TCAGAGATCC 
CTCCTGGAGC AGGACCTTGC TACCTACATC ACAGAATGCA GTAGCTTAAA GCGAAGTTTG 
GAGCAAGCGC GGATGGAGGT GTCCCAGGAG GATGACAAAG CTCTGCAGCT TCTCCACGAC 
ATCCGAGAGC AGAGCCGGAA GCTCCAGGAG ATCAAGGAGC AGGAGTACCA GGCTCAGGTG 
GAGGAGATGA GGCTGATGAT GAATCAGCTG GAAGAAGACC TGGTGTCAGC CCGCAGACGC 
AGCGATCTCT ACGAGTCTGA GCTGAGGGAG TCTCGGCTTG CCGCCGAGGA ATTCAAGCGG 
AAGGCAAACG AATGTCAGCA CAAACTGATG AAGGCTAAGG ACCAAGGGAA GCCTGAAGTG 
GGAGAATATT CCAAACTGGA GAAGATCAAT GCTGAGCAGC AGCTGAAGAT CCAGGAGCTC 
CAGGAGAAGC TGGAGAAGGC TGTAAAAGCC AGCACAGAGG CCACCGAGCT CCTGCAGAAT 
ATCCGCCAGG CAAAGGAGCG AGCAGAGCGG GAGCTAGAGA AGCTACACAA CCGGGAAGAC 
TCCTCCGAGG GCATCAAAAA GAAGCTGGTG GAAGCCGAGG AACGCCGCCA CTCCCTGGAG 
AACAAGGTAA AGAGACTAGA GACCATGGAG CGTAGAGAGA ACAGACTGAA GGATGACATC 
CAGACAAAGT CCGAACAGAT CCAGCAGATG GCTGATAAAA TTCTGGAGCT GGAGGAGAAA 
CATCGGGAGG CTCAGGTCTC AGCTCAACAT CTAGAAGTAC ACTTGAAACA GAAAGAACAG 
CACTACGAGG AAAAGATCAA GGTATTGGAC AATCAGATAA AGAAGGACCT GGCCGACAAG 
GAGAGCCTGG AGAACATGAT GCAGAGACAC GAGGAGGAGG CCCACGAGAA GGGCAAGATC 
CTCAGCGAGC AGAAGGCGAT GATCAACGCG ATGGATTCCA AGATCCGATC CCTGGAGCAG 
AGGATCGTGG AGCTGTCGGA AGCCAACAAG CTTGCGGCAA ACAGCAGTCT CTTCACCCAG 
AGGAACATGA AGGCCCAGGA AGAGATGATC TCAGAACTCA GGCAGCAGAA ATTTTACCTG 
GAGACGCAGG CCGGAAAGCT GGAGGCCCAG AACCGAAAGC TGGAAGAGCA ACTGGAGAAG 
ATCAGCCACC AAGATCACAG TGACAAGAGT CGGCTGCTGG AGCTGGAAAC AAGGCTGAGG 
GAGGTCAGCC TGGAGCACGA GGAGCAGAAG CTGGAGCTGA AGCGGCAGCT CACGGAGCTG 
CAGCTGTCCC TGCAGGAGCG CGAGTCCCAG CTGACGGCCC TGCAGGCCGC CCGGGCAGCG 
CTGGAGAGCC AGCTCCGCCA GGCGAAGACA GAGCTGGAGG AGACAACCGC GGAAGCGGAG 
GAGGAGATCC AGGCGCTCAC GGCACATCGA GATGAGATCC AGCGCAAATT CGATGCCCTT 
CGCAACAGCT GCACGGTCAT CACCGACCTG GAGGAGCAGC TGAACCAGCT CACCGAGGAC 
AACGCCGAGC TCAACAACCA AAACTTCTAC CTGTCCAAAC AACTCGATGA GGCTTCCGGG 
GCCAATGACG AGATTGTGCA GCTGCGGAGT GAGGTGGACC ATCTCCGCCG TGAGATCACG 
GAGCGGGAGA TGCAGCTCAC CAGCCAGAAG CAAGCACAAC TCAGTGCTCC TGATTTGCAG 
ACGATGGAGG CTCTGAAGAC GACATGCACC ATGCTGGAGG AGCAGGTCCT GGACCTGGAG 
GCCCTGAACG ACGAGCTGCT GGAGAAGGAG CGCCAGTGGG AGGCCTGGCG GAGCGTCCTC 
GGCGACGAGA AGTCCCAGTT CGAGTGTCGA GTTCGAGAGC TACAGAGGAT GCTGGACACC 
GAGAAGCAGA GCAGGGCTAG GGCCGATCAG CGGATCACCG AGTCGCGCCA GGTGGTGGAG 
TTGGCGGTGA AGGAACACAA GGCCGAGATT CTTGCTCTGC AGCAGGCTCT CAAGGAGCAG 
AAGCTCAAAG CCGAGAGCCT GTCGGACAAG CTCAACGACC TGGAGAAGAA GCACGCCATG 
CTGGAGATGA ACGCCCGGAG CTTACAGCAG AAACTAGAGA CAGAGCGGGA GCTCAAACAG 
AGGCTTCTGG AGGAGCAAGC CAAATTGCAG CAGCAGATGG ACCTGCAGAA GAACCACATC 
TTCAGACTGA CGCAAGGGCT GCAGGAGGCG CTGGACCGGG CGGATCTGCT GAAGACAGAA 
AGGAGCGACC TGGAGTACCA GCTGGAAAAC ATTCAGGTTC TCTACTCTCA CGAGAAAGTG 
AAAATGGAAG GCACAATCTC TCAGCAAACC AAACTCATTG ATTTCCTGCA AGCCAAAATG 
GACCAGCCTG CTAAAAAGAA AAAGGGTTTA TTTAGTCGAC GGAAAGAGGA CCCTGCTTTG 
CCCACACAGG TTCCTCTGCA GTACAATGAG CTGAAGCTAG CCCTGGAAAA GGAGAAAGCC 
CGATGCGCGG AGCTGGAGGA GGCCCTTCAG AAGACCCGCA TCGAACTCCG CTCTGCGCGG 
GAGGAAGCTG CCCACCGCAA AGCCACGGAC CACCCGCACC CGTCTACGCC AGCCACTGCG 
AGGCAGCAGA TCGCCATGTC TGCCATTGTG CGGTCGCCCG AGCACCAGCC CAGTGCCATG 
AGCCTGCTTG CCCCACCATC CAGCCGCAGA AAGGAGTCGT CAACTCCAGA GGAATTCAGC 
CGGCGTCTGA AAGAGCGCAT GCACCACAAC ATCCCTCACC GGTTTAATGT GGGCCTGAAC 
ATGAGAGCCA CCAAGTGCGC CGTGTGTCTG GATACTGTGC ACTTTGGACG CCAGGCATCC 
AAATGCCTCG AATGTCAAGT GATGTGTCAC CCCAAATGCT CCACCTGCTT GCCCGCTACC 
TGTGGCCTGC CAGCTGAATA TGCCACACAC TTCACTGAGG CCTTCTGCCG GGACAAAATG 
AACTCCCCGG GGCTCCAGAG CAAGGAGCCT GGCAGCAGCT TGCACCTGGA AGGGTGGATG 
AAAGTGCCCA GAAATAACAA ACGGGGACAG CAAGGCTGGG ACAGGAAGTA CATTGTTCTG 
GAGGGGTCAA AAGTCCTCAT CTATGACAAT GAAGCCAGAG AAGCTGGACA GAGGCCGGTG 
GAAGAATTTG AGCTGTGCCT TCCTGACGGG GACGTATCTA TTCATGGTGC CGTTGGTGCT 
TCAGAACTTG CAAATACCGC CAAAGCAGAT GTCCCATACA TCCTGAAGAT GGAGTCTCAC 
CCACACACCA CCTGCTGGCC TGGGAGAACC CTCTACTTGC TAGCACCCAG CTTCCCCGAC 
AAGCAGCGCT GGGTCACCGC CTTAGAATCT GTCGTCGCAG GTGGGAGAGT TTCTAGGGAA 
AAGGCCGAAG CCGATGCTGC ACGTGACCAT ACTTCGAGTG AGCATCAGCC AGTGTGGGTT 
GAGAAATTAC TTGGAAACTC TCTGCTGAAA CTGGAGGGCG ATGACCGGCT TGACATGAAC 
TGCACCCTGC CCTTCAGTGA CCAGGTAGTG CTGGTGGGCA CGGAGGAAGG CCTCTACGCC 
CTGAATGTCT TGAAAAACTC CTTAACCCAC ATCCCAGGGA TTGGCGCAGT CTTCCAAATT 
TACATCATCA AGGACCTGGA GAAGCTGCTC ATGATAGCAG GGGAAGAGCG GGCTCTGTGC 
CTGGTGGACG TGAAGAAGGT GAAGCAGTCC CTGGCGCAGT CACACCTGCC TGCCCAGCCC 
GACGTCTCCC CCAACATATT CGAAGCCGTC AAAGGCTGCC ACTTGTTCGC TGCTGGCAAG 
ATCGAGAACA GCCTGTGCAT CTGCGCCGCT ATGCCAAGCA AAGTCGTCAT CCTCCGCTAC 
AATGACAACC TCAGCAAGTA CTGCATCCGC AAGGAGATCG AGACCTCAGA GCCCTGCAGC 
TGTATCCACT TCACCAACTA CAGCATCCTC ATTGGGACCA ACAAATTCTA TGAGATCGAC 
ATGAAGCAGT ACACGCTTGA CGAGTTCCTG GACAAGAACG ACCATTCCTT GGCACCTGCT 
GTGTTCGCCT CCTCGTCCAA CAGCTTCCCT GTCTCCATTG TGCAGGCGAA CAGCGCCGGG 
CAGCGAGAAG AATACCTGCT GTGCTTCCAC GAATTTGGGG TGTTCGTGGA TTCTTACGGA 
AGACGTAGCC GCACAGATGA TCTCAAGTGG AGTCGCTTAC CTCTGGCCTT CGCCTACAGA 
GAACCTTATC TGTTTGTGAC TCACTTCAAC TCCCTGGAAG TCATTGAGAT CCAGGCCAGA 
TCCTCACTGG GGAGCCCTGC CCGAGCATAT CTGGAAATTC CAAACCCTCG CTACCTGGGC 
CCCGCGATTT CCTCCGGAGC GATTTACCTG GCCTCCTCAT ACCAGGACAA GTTAAGGGTC 
ATATGCTGCA AAGGAAACCT CGTGAAGGAG TCAGGCACTG AGCAGCACCG GGTGCCCTCC 
ACCTCCCGCA GCAGCCCCAA CAAGCGAGGC CCACCAACAT ACAACGAGCA CATCACCAAA 
CGCGTGGCCT CCAGCCCGGC GCCACCGGAA GGCCCCAGCC ACCCCCGAGA GCCAAGCACA 
CCGCACCGCT ACCGAGACAG AGAGGGTCGG ACAGAGCTGC GCAGGGACAA GTCTCCAGGC 
CGCCCTCTGG AGCGGGAGAA GTCCCCAGGC CGAATGCTCA GCACTAGGAG AGAGCGGTCC 
CCAGGGAGAC TGTTTGAAGA CAGCAGCAGG GGCCGGCTGC CTGCAGGAGC AGTGAGGACC 
CCACTGTCCC AGGTTAACAA GGTCTGGGAC CAGTCTTCAG TATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006530201.1
CDS	158..6442
Translation	MLKFKYGVRNPPEASASEPIASRASRLNLFFQGKPPLMTQQQMSALSREGMLDALFALFEECSQPALMKMKHVSSFVQKYSDTIAELRELQPSARDFEVRSLVGCGHFAEVQVVREKATGDVYAMKIMKKKALLAQEQVSFFEEERNILSRSTSPWIPQLQYAFQDKNNLYLVMEYQPGGDLLSLLNRYEDQLDESMIQFYLAELILAVHSVHQMGYVHRDIKPENILIDRTGHIKLVDFGSAAKMNSNKVDAKLPIGTPDYMAPEVLTVMNEDRRGTYGLDCDWWSVGVVAYEMVYGKTPFTEGTSARTFNNIMNFQRFLKFPDDPKVSSELLDLLQSLLCVQKERLKFEGLCCHPFFARTDWNNIRNSPPPFVPTLKSDDDTSNFDEPEKNSWVSSSVCQLSPSGFSGEELPFVGFSYSKALGYLGRSESVVSSLDSPAKVSSMEKKLLIKSKELQDSQDKCHKMEQEMTRLHRRVSEVEAVLSQKEVELKASETQRSLLEQDLATYITECSSLKRSLEQARMEVSQEDDKALQLLHDIREQSRKLQEIKEQEYQAQVEEMRLMMNQLEEDLVSARRRSDLYESELRESRLAAEEFKRKANECQHKLMKAKDQGKPEVGEYSKLEKINAEQQLKIQELQEKLEKAVKASTEATELLQNIRQAKERAERELEKLHNREDSSEGIKKKLVEAEERRHSLENKVKRLETMERRENRLKDDIQTKSEQIQQMADKILELEEKHREAQVSAQHLEVHLKQKEQHYEEKIKVLDNQIKKDLADKESLENMMQRHEEEAHEKGKILSEQKAMINAMDSKIRSLEQRIVELSEANKLAANSSLFTQRNMKAQEEMISELRQQKFYLETQAGKLEAQNRKLEEQLEKISHQDHSDKSRLLELETRLREVSLEHEEQKLELKRQLTELQLSLQERESQLTALQAARAALESQLRQAKTELEETTAEAEEEIQALTAHRDEIQRKFDALRNSCTVITDLEEQLNQLTEDNAELNNQNFYLSKQLDEASGANDEIVQLRSEVDHLRREITEREMQLTSQKQAQLSAPDLQTMEALKTTCTMLEEQVLDLEALNDELLEKERQWEAWRSVLGDEKSQFECRVRELQRMLDTEKQSRARADQRITESRQVVELAVKEHKAEILALQQALKEQKLKAESLSDKLNDLEKKHAMLEMNARSLQQKLETERELKQRLLEEQAKLQQQMDLQKNHIFRLTQGLQEALDRADLLKTERSDLEYQLENIQVLYSHEKVKMEGTISQQTKLIDFLQAKMDQPAKKKKGLFSRRKEDPALPTQVPLQYNELKLALEKEKARCAELEEALQKTRIELRSAREEAAHRKATDHPHPSTPATARQQIAMSAIVRSPEHQPSAMSLLAPPSSRRKESSTPEEFSRRLKERMHHNIPHRFNVGLNMRATKCAVCLDTVHFGRQASKCLECQVMCHPKCSTCLPATCGLPAEYATHFTEAFCRDKMNSPGLQSKEPGSSLHLEGWMKVPRNNKRGQQGWDRKYIVLEGSKVLIYDNEAREAGQRPVEEFELCLPDGDVSIHGAVGASELANTAKADVPYILKMESHPHTTCWPGRTLYLLAPSFPDKQRWVTALESVVAGGRVSREKAEADAARDHTSSEHQPVWVEKLLGNSLLKLEGDDRLDMNCTLPFSDQVVLVGTEEGLYALNVLKNSLTHIPGIGAVFQIYIIKDLEKLLMIAGEERALCLVDVKKVKQSLAQSHLPAQPDVSPNIFEAVKGCHLFAAGKIENSLCICAAMPSKVVILRYNDNLSKYCIRKEIETSEPCSCIHFTNYSILIGTNKFYEIDMKQYTLDEFLDKNDHSLAPAVFASSSNSFPVSIVQANSAGQREEYLLCFHEFGVFVDSYGRRSRTDDLKWSRLPLAFAYREPYLFVTHFNSLEVIEIQARSSLGSPARAYLEIPNPRYLGPAISSGAIYLASSYQDKLRVICCKGNLVKESGTEQHRVPSTSRSSPNKRGPPTYNEHITKRVASSPAPPEGPSHPREPSTPHRYRDREGRTELRRDKSPGRPLEREKSPGRMLSTRRERSPGRLFEDSSRGRLPAGAVRTPLSQVNKVWDQSSV

Target ORF information:

RefSeq Version	XM_006530138.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus citron (Cit), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006530138.4

ORF Insert Sequence:

ATGTTGAAGT TCAAGTATGG TGTGCGGAAC CCGCCGGAGG CCAGTGCCTC CGAGCCCATT 
GCCAGTCGGG CCTCCAGGCT AAATCTCTTC TTCCAGGGGA AACCGCCCCT CATGACTCAA 
CAGCAGATGT CTGCTCTTTC CCGGGAAGGG ATGCTAGACG CCCTCTTCGC TCTCTTTGAA 
GAGTGCAGCC AACCCGCCCT GATGAAGATG AAGCACGTGA GCAGCTTTGT CCAGAAGTAT 
TCCGACACCA TAGCCGAGTT GCGGGAGCTG CAGCCGTCGG CGAGAGACTT CGAAGTTCGA 
AGCCTTGTGG GCTGTGGTCA CTTCGCTGAA GTGCAGGTGG TTAGAGAGAA GGCGACCGGG 
GACGTCTATG CCATGAAAAT CATGAAGAAG AAGGCTTTGC TGGCCCAGGA ACAGGTTTCA 
TTTTTCGAGG AGGAGAGGAA CATATTATCT CGGAGCACGA GTCCTTGGAT CCCCCAGTTA 
CAGTACGCCT TTCAGGACAA AAATAACCTT TACCTGGTCA TGGAATATCA GCCTGGAGGG 
GATTTGCTGT CGCTTCTGAA CAGATACGAG GACCAATTAG ATGAGAGCAT GATCCAGTTT 
TACCTTGCTG AGCTGATTTT GGCTGTCCAC AGCGTGCACC AGATGGGATA TGTGCATCGA 
GACATCAAGC CCGAGAACAT CCTCATCGAC CGGACGGGAC ACATCAAGCT GGTGGATTTT 
GGATCAGCCG CTAAGATGAA TTCAAATAAG GTGGATGCCA AACTCCCCAT TGGGACCCCG 
GATTACATGG CTCCGGAAGT GTTGACCGTG ATGAACGAGG ACCGAAGGGG CACATACGGC 
TTGGACTGTG ACTGGTGGTC TGTCGGAGTT GTTGCTTATG AGATGGTTTA TGGGAAGACC 
CCATTCACAG AGGGAACCTC CGCCCGGACC TTCAACAACA TCATGAACTT CCAGCGGTTT 
TTGAAGTTCC CAGATGACCC CAAAGTTAGC AGTGAGCTCC TTGATCTGCT TCAGAGTCTG 
CTGTGTGTCC AGAAAGAGAG ACTGAAGTTC GAGGGTCTCT GCTGCCACCC TTTCTTTGCC 
AGAACGGACT GGAACAACAT CCGTAACTCT CCTCCCCCCT TCGTCCCCAC CCTCAAGTCT 
GACGATGACA CCTCCAATTT TGATGAACCA GAGAAGAATT CGTGGGTTTC ATCCTCTGTG 
TGCCAGCTGA GCCCCTCGGG CTTCTCAGGC GAAGAGCTGC CGTTTGTGGG ATTTTCGTAC 
AGCAAGGCAC TGGGGTATCT TGGTAGATCT GAGTCTGTCG TGTCGAGTCT GGACTCCCCT 
GCCAAGGTTA GCTCCATGGA AAAGAAACTT CTCATCAAAA GCAAAGAGCT CCAAGACTCC 
CAGGACAAGT GTCACAAGAT GGAGCAGGAA ATGACCCGGT TACATCGCAG AGTGTCAGAG 
GTGGAGGCTG TGCTTAGTCA GAAGGAGGTG GAGCTGAAGG CCTCTGAGAC TCAGAGATCC 
CTCCTGGAGC AGGACCTTGC TACCTACATC ACAGAATGCA GTAGCTTAAA GCGAAGTTTG 
GAGCAAGCGC GGATGGAGGT GTCCCAGGAG GATGACAAAG CTCTGCAGCT TCTCCACGAC 
ATCCGAGAGC AGAGCCGGAA GCTCCAGGAG ATCAAGGAGC AGGAGTACCA GGCTCAGGTG 
GAGGAGATGA GGCTGATGAT GAATCAGCTG GAAGAAGACC TGGTGTCAGC CCGCAGACGC 
AGCGATCTCT ACGAGTCTGA GCTGAGGGAG TCTCGGCTTG CCGCCGAGGA ATTCAAGCGG 
AAGGCAAACG AATGTCAGCA CAAACTGATG AAGGCTAAGG ACCAAGGGAA GCCTGAAGTG 
GGAGAATATT CCAAACTGGA GAAGATCAAT GCTGAGCAGC AGCTGAAGAT CCAGGAGCTC 
CAGGAGAAGC TGGAGAAGGC TGTAAAAGCC AGCACAGAGG CCACCGAGCT CCTGCAGAAT 
ATCCGCCAGG CAAAGGAGCG AGCAGAGCGG GAGCTAGAGA AGCTACACAA CCGGGAAGAC 
TCCTCCGAGG GCATCAAAAA GAAGCTGGTG GAAGCCGAGG AACGCCGCCA CTCCCTGGAG 
AACAAGGTAA AGAGACTAGA GACCATGGAG CGTAGAGAGA ACAGACTGAA GGATGACATC 
CAGACAAAGT CCGAACAGAT CCAGCAGATG GCTGATAAAA TTCTGGAGCT GGAGGAGAAA 
CATCGGGAGG CTCAGGTCTC AGCTCAACAT CTAGAAGTAC ACTTGAAACA GAAAGAACAG 
CACTACGAGG AAAAGATCAA GGTATTGGAC AATCAGATAA AGAAGGACCT GGCCGACAAG 
GAGAGCCTGG AGAACATGAT GCAGAGACAC GAGGAGGAGG CCCACGAGAA GGGCAAGATC 
CTCAGCGAGC AGAAGGCGAT GATCAACGCG ATGGATTCCA AGATCCGATC CCTGGAGCAG 
AGGATCGTGG AGCTGTCGGA AGCCAACAAG CTTGCGGCAA ACAGCAGTCT CTTCACCCAG 
AGGAACATGA AGGCCCAGGA AGAGATGATC TCAGAACTCA GGCAGCAGAA ATTTTACCTG 
GAGACGCAGG CCGGAAAGCT GGAGGCCCAG AACCGAAAGC TGGAAGAGCA ACTGGAGAAG 
ATCAGCCACC AAGATCACAG TGACAAGAGT CGGCTGCTGG AGCTGGAAAC AAGGCTGAGG 
GAGGTCAGCC TGGAGCACGA GGAGCAGAAG CTGGAGCTGA AGCGGCAGCT CACGGAGCTG 
CAGCTGTCCC TGCAGGAGCG CGAGTCCCAG CTGACGGCCC TGCAGGCCGC CCGGGCAGCG 
CTGGAGAGCC AGCTCCGCCA GGCGAAGACA GAGCTGGAGG AGACAACCGC GGAAGCGGAG 
GAGGAGATCC AGGCGCTCAC GGCACATCGA GATGAGATCC AGCGCAAATT CGATGCCCTT 
CGCAACAGCT GCACGGTCAT CACCGACCTG GAGGAGCAGC TGAACCAGCT CACCGAGGAC 
AACGCCGAGC TCAACAACCA AAACTTCTAC CTGTCCAAAC AACTCGATGA GGCTTCCGGG 
GCCAATGACG AGATTGTGCA GCTGCGGAGT GAGGTGGACC ATCTCCGCCG TGAGATCACG 
GAGCGGGAGA TGCAGCTCAC CAGCCAGAAG CAAGCACAAC TCAGTGCTCC TGATTTGCAG 
ACGATGGAGG CTCTGAAGAC GACATGCACC ATGCTGGAGG AGCAGGTCCT GGACCTGGAG 
GCCCTGAACG ACGAGCTGCT GGAGAAGGAG CGCCAGTGGG AGGCCTGGCG GAGCGTCCTC 
GGCGACGAGA AGTCCCAGTT CGAGTGTCGA GTTCGAGAGC TACAGAGGAT GCTGGACACC 
GAGAAGCAGA GCAGGGCTAG GGCCGATCAG CGGATCACCG AGTCGCGCCA GGTGGTGGAG 
TTGGCGGTGA AGGAACACAA GGCCGAGATT CTTGCTCTGC AGCAGGCTCT CAAGGAGCAG 
AAGCTCAAAG CCGAGAGCCT GTCGGACAAG CTCAACGACC TGGAGAAGAA GCACGCCATG 
CTGGAGATGA ACGCCCGGAG CTTACAGCAG AAACTAGAGA CAGAGCGGGA GCTCAAACAG 
AGGCTTCTGG AGGAGCAAGC CAAATTGCAG CAGCAGATGG ACCTGCAGAA GAACCACATC 
TTCAGACTGA CGCAAGGGCT GCAGGAGGCG CTGGACCGGG CGGATCTGCT GAAGACAGAA 
AGGAGCGACC TGGAGTACCA GCTGGAAAAC ATTCAGGTTC TCTACTCTCA CGAGAAAGTG 
AAAATGGAAG GCACAATCTC TCAGCAAACC AAACTCATTG ATTTCCTGCA AGCCAAAATG 
GACCAGCCTG CTAAAAAGAA AAAGGGTTTA TTTAGTCGAC GGAAAGAGGA CCCTGCTTTG 
CCCACACAGG TTCCTCTGCA GTACAATGAG CTGAAGCTAG CCCTGGAAAA GGAGAAAGCC 
CGATGCGCGG AGCTGGAGGA GGCCCTTCAG AAGACCCGCA TCGAACTCCG CTCTGCGCGG 
GAGGAAGCTG CCCACCGCAA AGCCACGGAC CACCCGCACC CGTCTACGCC AGCCACTGCG 
AGGCAGCAGA TCGCCATGTC TGCCATTGTG CGGTCGCCCG AGCACCAGCC CAGTGCCATG 
AGCCTGCTTG CCCCACCATC CAGCCGCAGA AAGGAGTCGT CAACTCCAGA GGAATTCAGC 
CGGCGTCTGA AAGAGCGCAT GCACCACAAC ATCCCTCACC GGTTTAATGT GGGCCTGAAC 
ATGAGAGCCA CCAAGTGCGC CGTGTGTCTG GATACTGTGC ACTTTGGACG CCAGGCATCC 
AAATGCCTCG AATGTCAAGT GATGTGTCAC CCCAAATGCT CCACCTGCTT GCCCGCTACC 
TGTGGCCTGC CAGCTGAATA TGCCACACAC TTCACTGAGG CCTTCTGCCG GGACAAAATG 
AACTCCCCGG GGCTCCAGAG CAAGGAGCCT GGCAGCAGCT TGCACCTGGA AGGGTGGATG 
AAAGTGCCCA GAAATAACAA ACGGGGACAG CAAGGCTGGG ACAGGAAGTA CATTGTTCTG 
GAGGGGTCAA AAGTCCTCAT CTATGACAAT GAAGCCAGAG AAGCTGGACA GAGGCCGGTG 
GAAGAATTTG AGCTGTGCCT TCCTGACGGG GACGTATCTA TTCATGGTGC CGTTGGTGCT 
TCAGAACTTG CAAATACCGC CAAAGCAGAT GTCCCATACA TCCTGAAGAT GGAGTCTCAC 
CCACACACCA CCTGCTGGCC TGGGAGAACC CTCTACTTGC TAGCACCCAG CTTCCCCGAC 
AAGCAGCGCT GGGTCACCGC CTTAGAATCT GTCGTCGCAG GTGGGAGAGT TTCTAGGGAA 
AAGGCCGAAG CCGATGCTGC ACGTGACCAT ACTTCGAGTG AGCATCAGCC AGTGTGGGTT 
GAGAAATTAC TTGGAAACTC TCTGCTGAAA CTGGAGGGCG ATGACCGGCT TGACATGAAC 
TGCACCCTGC CCTTCAGTGA CCAGGTAGTG CTGGTGGGCA CGGAGGAAGG CCTCTACGCC 
CTGAATGTCT TGAAAAACTC CTTAACCCAC ATCCCAGGGA TTGGCGCAGT CTTCCAAATT 
TACATCATCA AGGACCTGGA GAAGCTGCTC ATGATAGCAG GGGAAGAGCG GGCTCTGTGC 
CTGGTGGACG TGAAGAAGGT GAAGCAGTCC CTGGCGCAGT CACACCTGCC TGCCCAGCCC 
GACGTCTCCC CCAACATATT CGAAGCCGTC AAAGGCTGCC ACTTGTTCGC TGCTGGCAAG 
ATCGAGAACA GCCTGTGCAT CTGCGCCGCT ATGCCAAGCA AAGTCGTCAT CCTCCGCTAC 
AATGACAACC TCAGCAAGTA CTGCATCCGC AAGGAGATCG AGACCTCAGA GCCCTGCAGC 
TGTATCCACT TCACCAACTA CAGCATCCTC ATTGGGACCA ACAAATTCTA TGAGATCGAC 
ATGAAGCAGT ACACGCTTGA CGAGTTCCTG GACAAGAACG ACCATTCCTT GGCACCTGCT 
GTGTTCGCCT CCTCGTCCAA CAGCTTCCCT GTCTCCATTG TGCAGGCGAA CAGCGCCGGG 
CAGCGAGAAG AATACCTGCT GTGCTTCCAC GAATTTGGGG TGTTCGTGGA TTCTTACGGA 
AGACGTAGCC GCACAGATGA TCTCAAGTGG AGTCGCTTAC CTCTGGCCTT CGCCTACAGA 
GAACCTTATC TGTTTGTGAC TCACTTCAAC TCCCTGGAAG TCATTGAGAT CCAGGCCAGA 
TCCTCACTGG GGAGCCCTGC CCGAGCATAT CTGGAAATTC CAAACCCTCG CTACCTGGGC 
CCCGCGATTT CCTCCGGAGC GATTTACCTG GCCTCCTCAT ACCAGGACAA GTTAAGGGTC 
ATATGCTGCA AAGGAAACCT CGTGAAGGAG TCAGGCACTG AGCAGCACCG GGTGCCCTCC 
ACCTCCCGCA GCAGCCCCAA CAAGCGAGGC CCACCAACAT ACAACGAGCA CATCACCAAA 
CGCGTGGCCT CCAGCCCGGC GCCACCGGAA GGCCCCAGCC ACCCCCGAGA GCCAAGCACA 
CCGCACCGCT ACCGAGACAG AGAGGGTCGG ACAGAGCTGC GCAGGGACAA GTCTCCAGGC 
CGCCCTCTGG AGCGGGAGAA GTCCCCAGGC CGAATGCTCA GCACTAGGAG AGAGCGGTCC 
CCAGGGAGAC TGTTTGAAGA CAGCAGCAGG GGCCGGCTGC CTGCAGGAGC AGTGAGGACC 
CCACTGTCCC AGGTTAACAA GGTCTGGGAC CAGTCTTCAG TATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cit ( XM_006530139.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006530202.1 Mus musculus citron (Cit), transcript variant X13, mRNA.

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CloneID	OMu35780
Clone ID Related Accession (Same CDS sequence)	XM_006530139.3 , XM_006530139.4
Accession Version	XM_006530139.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6246bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	citron Rho-interacting kinase isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000071.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006530139.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTGAAGT TCAAGTATGG TGTGCGGAAC CCGCCGGAGG CCAGTGCCTC CGAGCCCATT 
GCCAGTCGGG CCTCCAGGCT AAATCTCTTC TTCCAGGGGA AACCGCCCCT CATGACTCAA 
CAGCAGATGT CTGCTCTTTC CCGGGAAGGG ATGCTAGACG CCCTCTTCGC TCTCTTTGAA 
GAGTGCAGCC AACCCGCCCT GATGAAGATG AAGCACGTGA GCAGCTTTGT CCAGAAGTAT 
TCCGACACCA TAGCCGAGTT GCGGGAGCTG CAGCCGTCGG CGAGAGACTT CGAAGTTCGA 
AGCCTTGTGG GCTGTGGTCA CTTCGCTGAA GTGCAGGTGG TTAGAGAGAA GGCGACCGGG 
GACGTCTATG CCATGAAAAT CATGAAGAAG AAGGCTTTGC TGGCCCAGGA ACAGGTTTCA 
TTTTTCGAGG AGGAGAGGAA CATATTATCT CGGAGCACGA GTCCTTGGAT CCCCCAGTTA 
CAGTACGCCT TTCAGGACAA AAATAACCTT TACCTGGTCA TGGAATATCA GCCTGGAGGG 
GATTTGCTGT CGCTTCTGAA CAGATACGAG GACCAATTAG ATGAGAGCAT GATCCAGTTT 
TACCTTGCTG AGCTGATTTT GGCTGTCCAC AGCGTGCACC AGATGGGATA TGTGCATCGA 
GACATCAAGC CCGAGAACAT CCTCATCGAC CGGACGGGAC ACATCAAGCT GGTGGATTTT 
GGATCAGCCG CTAAGATGAA TTCAAATAAG GTGGATGCCA AACTCCCCAT TGGGACCCCG 
GATTACATGG CTCCGGAAGT GTTGACCGTG ATGAACGAGG ACCGAAGGGG CACATACGGC 
TTGGACTGTG ACTGGTGGTC TGTCGGAGTT GTTGCTTATG AGATGGTTTA TGGGAAGACC 
CCATTCACAG AGGGAACCTC CGCCCGGACC TTCAACAACA TCATGAACTT CCAGCGGTTT 
TTGAAGTTCC CAGATGACCC CAAAGTTAGC AGTGAGCTCC TTGATCTGCT TCAGAGTCTG 
CTGTGTGTCC AGAAAGAGAG ACTGAAGTTC GAGGGTCTCT GCTGCCACCC TTTCTTTGCC 
AGAACGGACT GGAACAACAT CCGTAACTCT CCTCCCCCCT TCGTCCCCAC CCTCAAGTCT 
GACGATGACA CCTCCAATTT TGATGAACCA GAGAAGAATT CGTGGGTTTC ATCCTCTGTG 
TGCCAGCTGA GCCCCTCGGG CTTCTCAGGC GAAGAGCTGC CGTTTGTGGG ATTTTCGTAC 
AGCAAGGCAC TGGGGTATCT TGGTAGATCT GAGTCTGTCG TGTCGAGTCT GGACTCCCCT 
GCCAAGGTTA GCTCCATGGA AAAGAAACTT CTCATCAAAA GCAAAGAGCT CCAAGACTCC 
CAGGACAAGT GTCACAAGAT GGAGCAGGAA ATGACCCGGT TACATCGCAG AGTGTCAGAG 
GTGGAGGCTG TGCTTAGTCA GAAGGAGGTG GAGCTGAAGG CCTCTGAGAC TCAGAGATCC 
CTCCTGGAGC AGGACCTTGC TACCTACATC ACAGAATGCA GTAGCTTAAA GCGAAGTTTG 
GAGCAAGCGC GGATGGAGGT GTCCCAGGAG GATGACAAAG CTCTGCAGCT TCTCCACGAC 
ATCCGAGAGC AGAGCCGGAA GCTCCAGGAG ATCAAGGAGC AGGAGTACCA GGCTCAGGTG 
GAGGAGATGA GGCTGATGAT GAATCAGCTG GAAGAAGACC TGGTGTCAGC CCGCAGACGC 
AGCGATCTCT ACGAGTCTGA GCTGAGGGAG TCTCGGCTTG CCGCCGAGGA ATTCAAGCGG 
AAGGCAAACG AATGTCAGCA CAAACTGATG AAGGTAGTCA GCCACCCTCC AAGAGGGGAC 
TCCGGGGGCA CGGCCCTGGA TGATCTTCAC AAGATGCAAG GCCACGCTGG CTTAACGAGT 
GCTAAGGACC AAGGGAAGCC TGAAGTGGGA GAATATTCCA AACTGGAGAA GATCAATGCT 
GAGCAGCAGC TGAAGATCCA GGAGCTCCAG GAGAAGCTGG AGAAGGCTGT AAAAGCCAGC 
ACAGAGGCCA CCGAGCTCCT GCAGAATATC CGCCAGGCAA AGGAGCGAGC AGAGCGGGAG 
CTAGAGAAGC TACACAACCG GGAAGACTCC TCCGAGGGCA TCAAAAAGAA GCTGGTGGAA 
GCCGAGGAGC TGGAGGAGAA ACATCGGGAG GCTCAGGTCT CAGCTCAACA TCTAGAAGTA 
CACTTGAAAC AGAAAGAACA GCACTACGAG GAAAAGATCA AGGTATTGGA CAATCAGATA 
AAGAAGGACC TGGCCGACAA GGAGAGCCTG GAGAACATGA TGCAGAGACA CGAGGAGGAG 
GCCCACGAGA AGGGCAAGAT CCTCAGCGAG CAGAAGGCGA TGATCAACGC GATGGATTCC 
AAGATCCGAT CCCTGGAGCA GAGGATCGTG GAGCTGTCGG AAGCCAACAA GCTTGCGGCA 
AACAGCAGTC TCTTCACCCA GAGGAACATG AAGGCCCAGG AAGAGATGAT CTCAGAACTC 
AGGCAGCAGA AATTTTACCT GGAGACGCAG GCCGGAAAGC TGGAGGCCCA GAACCGAAAG 
CTGGAAGAGC AACTGGAGAA GATCAGCCAC CAAGATCACA GTGACAAGAG TCGGCTGCTG 
GAGCTGGAAA CAAGGCTGAG GGAGGTCAGC CTGGAGCACG AGGAGCAGAA GCTGGAGCTG 
AAGCGGCAGC TCACGGAGCT GCAGCTGTCC CTGCAGGAGC GCGAGTCCCA GCTGACGGCC 
CTGCAGGCCG CCCGGGCAGC GCTGGAGAGC CAGCTCCGCC AGGCGAAGAC AGAGCTGGAG 
GAGACAACCG CGGAAGCGGA GGAGGAGATC CAGGCGCTCA CGGCACATCG AGATGAGATC 
CAGCGCAAAT TCGATGCCCT TCGCAACAGC TGCACGGTCA TCACCGACCT GGAGGAGCAG 
CTGAACCAGC TCACCGAGGA CAACGCCGAG CTCAACAACC AAAACTTCTA CCTGTCCAAA 
CAACTCGATG AGGCTTCCGG GGCCAATGAC GAGATTGTGC AGCTGCGGAG TGAGGTGGAC 
CATCTCCGCC GTGAGATCAC GGAGCGGGAG ATGCAGCTCA CCAGCCAGAA GCAAGCACAA 
CTCAGTGCTC CTGATTTGCA GACGATGGAG GCTCTGAAGA CGACATGCAC CATGCTGGAG 
GAGCAGGTCC TGGACCTGGA GGCCCTGAAC GACGAGCTGC TGGAGAAGGA GCGCCAGTGG 
GAGGCCTGGC GGAGCGTCCT CGGCGACGAG AAGTCCCAGT TCGAGTGTCG AGTTCGAGAG 
CTACAGAGGA TGCTGGACAC CGAGAAGCAG AGCAGGGCTA GGGCCGATCA GCGGATCACC 
GAGTCGCGCC AGGTGGTGGA GTTGGCGGTG AAGGAACACA AGGCCGAGAT TCTTGCTCTG 
CAGCAGGCTC TCAAGGAGCA GAAGCTCAAA GCCGAGAGCC TGTCGGACAA GCTCAACGAC 
CTGGAGAAGA AGCACGCCAT GCTGGAGATG AACGCCCGGA GCTTACAGCA GAAACTAGAG 
ACAGAGCGGG AGCTCAAACA GAGGCTTCTG GAGGAGCAAG CCAAATTGCA GCAGCAGATG 
GACCTGCAGA AGAACCACAT CTTCAGACTG ACGCAAGGGC TGCAGGAGGC GCTGGACCGG 
GCGGATCTGC TGAAGACAGA AAGGAGCGAC CTGGAGTACC AGCTGGAAAA CATTCAGGTT 
CTCTACTCTC ACGAGAAAGT GAAAATGGAA GGCACAATCT CTCAGCAAAC CAAACTCATT 
GATTTCCTGC AAGCCAAAAT GGACCAGCCT GCTAAAAAGA AAAAGGGTTT ATTTAGTCGA 
CGGAAAGAGG ACCCTGCTTT GCCCACACAG GTTCCTCTGC AGTACAATGA GCTGAAGCTA 
GCCCTGGAAA AGGAGAAAGC CCGATGCGCG GAGCTGGAGG AGGCCCTTCA GAAGACCCGC 
ATCGAACTCC GCTCTGCGCG GGAGGAAGCT GCCCACCGCA AAGCCACGGA CCACCCGCAC 
CCGTCTACGC CAGCCACTGC GAGGCAGCAG ATCGCCATGT CTGCCATTGT GCGGTCGCCC 
GAGCACCAGC CCAGTGCCAT GAGCCTGCTT GCCCCACCAT CCAGCCGCAG AAAGGAGTCG 
TCAACTCCAG AGGAATTCAG CCGGCGTCTG AAAGAGCGCA TGCACCACAA CATCCCTCAC 
CGGTTTAATG TGGGCCTGAA CATGAGAGCC ACCAAGTGCG CCGTGTGTCT GGATACTGTG 
CACTTTGGAC GCCAGGCATC CAAATGCCTC GAATGTCAAG TGATGTGTCA CCCCAAATGC 
TCCACCTGCT TGCCCGCTAC CTGTGGCCTG CCAGCTGAAT ATGCCACACA CTTCACTGAG 
GCCTTCTGCC GGGACAAAAT GAACTCCCCG GGGCTCCAGA GCAAGGAGCC TGGCAGCAGC 
TTGCACCTGG AAGGGTGGAT GAAAGTGCCC AGAAATAACA AACGGGGACA GCAAGGCTGG 
GACAGGAAGT ACATTGTTCT GGAGGGGTCA AAAGTCCTCA TCTATGACAA TGAAGCCAGA 
GAAGCTGGAC AGAGGCCGGT GGAAGAATTT GAGCTGTGCC TTCCTGACGG GGACGTATCT 
ATTCATGGTG CCGTTGGTGC TTCAGAACTT GCAAATACCG CCAAAGCAGA TGTCCCATAC 
ATCCTGAAGA TGGAGTCTCA CCCACACACC ACCTGCTGGC CTGGGAGAAC CCTCTACTTG 
CTAGCACCCA GCTTCCCCGA CAAGCAGCGC TGGGTCACCG CCTTAGAATC TGTCGTCGCA 
GGTGGGAGAG TTTCTAGGGA AAAGGCCGAA GCCGATGCTG CACGTGACCA TACTTCGAGT 
GAGCATCAGC CAGTGTGGGT TGAGAAATTA CTTGGAAACT CTCTGCTGAA ACTGGAGGGC 
GATGACCGGC TTGACATGAA CTGCACCCTG CCCTTCAGTG ACCAGGTAGT GCTGGTGGGC 
ACGGAGGAAG GCCTCTACGC CCTGAATGTC TTGAAAAACT CCTTAACCCA CATCCCAGGG 
ATTGGCGCAG TCTTCCAAAT TTACATCATC AAGGACCTGG AGAAGCTGCT CATGATAGCA 
GGGGAAGAGC GGGCTCTGTG CCTGGTGGAC GTGAAGAAGG TGAAGCAGTC CCTGGCGCAG 
TCACACCTGC CTGCCCAGCC CGACGTCTCC CCCAACATAT TCGAAGCCGT CAAAGGCTGC 
CACTTGTTCG CTGCTGGCAA GATCGAGAAC AGCCTGTGCA TCTGCGCCGC TATGCCAAGC 
AAAGTCGTCA TCCTCCGCTA CAATGACAAC CTCAGCAAGT ACTGCATCCG CAAGGAGATC 
GAGACCTCAG AGCCCTGCAG CTGTATCCAC TTCACCAACT ACAGCATCCT CATTGGGACC 
AACAAATTCT ATGAGATCGA CATGAAGCAG TACACGCTTG ACGAGTTCCT GGACAAGAAC 
GACCATTCCT TGGCACCTGC TGTGTTCGCC TCCTCGTCCA ACAGCTTCCC TGTCTCCATT 
GTGCAGGCGA ACAGCGCCGG GCAGCGAGAA GAATACCTGC TGTGCTTCCA CGAATTTGGG 
GTGTTCGTGG ATTCTTACGG AAGACGTAGC CGCACAGATG ATCTCAAGTG GAGTCGCTTA 
CCTCTGGCCT TCGCCTACAG AGAACCTTAT CTGTTTGTGA CTCACTTCAA CTCCCTGGAA 
GTCATTGAGA TCCAGGCCAG ATCCTCACTG GGGAGCCCTG CCCGAGCATA TCTGGAAATT 
CCAAACCCTC GCTACCTGGG CCCCGCGATT TCCTCCGGAG CGATTTACCT GGCCTCCTCA 
TACCAGGACA AGTTAAGGGT CATATGCTGC AAAGGAAACC TCGTGAAGGA GTCAGGCACT 
GAGCAGCACC GGGTGCCCTC CACCTCCCGC AGCAGCCCCA ACAAGCGAGG CCCACCAACA 
TACAACGAGC ACATCACCAA ACGCGTGGCC TCCAGCCCGG CGCCACCGGA AGGCCCCAGC 
CACCCCCGAG AGCCAAGCAC ACCGCACCGC TACCGAGACA GAGAGGGTCG GACAGAGCTG 
CGCAGGGACA AGTCTCCAGG CCGCCCTCTG GAGCGGGAGA AGTCCCCAGG CCGAATGCTC 
AGCACTAGGA GAGAGCGGTC CCCAGGGAGA CTGTTTGAAG ACAGCAGCAG GGGCCGGCTG 
CCTGCAGGAG CAGTGAGGAC CCCACTGTCC CAGGTTAACA AGGTCTGGGA CCAGTCTTCA 
GTATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006530202.1
CDS	158..6403
Translation	MLKFKYGVRNPPEASASEPIASRASRLNLFFQGKPPLMTQQQMSALSREGMLDALFALFEECSQPALMKMKHVSSFVQKYSDTIAELRELQPSARDFEVRSLVGCGHFAEVQVVREKATGDVYAMKIMKKKALLAQEQVSFFEEERNILSRSTSPWIPQLQYAFQDKNNLYLVMEYQPGGDLLSLLNRYEDQLDESMIQFYLAELILAVHSVHQMGYVHRDIKPENILIDRTGHIKLVDFGSAAKMNSNKVDAKLPIGTPDYMAPEVLTVMNEDRRGTYGLDCDWWSVGVVAYEMVYGKTPFTEGTSARTFNNIMNFQRFLKFPDDPKVSSELLDLLQSLLCVQKERLKFEGLCCHPFFARTDWNNIRNSPPPFVPTLKSDDDTSNFDEPEKNSWVSSSVCQLSPSGFSGEELPFVGFSYSKALGYLGRSESVVSSLDSPAKVSSMEKKLLIKSKELQDSQDKCHKMEQEMTRLHRRVSEVEAVLSQKEVELKASETQRSLLEQDLATYITECSSLKRSLEQARMEVSQEDDKALQLLHDIREQSRKLQEIKEQEYQAQVEEMRLMMNQLEEDLVSARRRSDLYESELRESRLAAEEFKRKANECQHKLMKVVSHPPRGDSGGTALDDLHKMQGHAGLTSAKDQGKPEVGEYSKLEKINAEQQLKIQELQEKLEKAVKASTEATELLQNIRQAKERAERELEKLHNREDSSEGIKKKLVEAEELEEKHREAQVSAQHLEVHLKQKEQHYEEKIKVLDNQIKKDLADKESLENMMQRHEEEAHEKGKILSEQKAMINAMDSKIRSLEQRIVELSEANKLAANSSLFTQRNMKAQEEMISELRQQKFYLETQAGKLEAQNRKLEEQLEKISHQDHSDKSRLLELETRLREVSLEHEEQKLELKRQLTELQLSLQERESQLTALQAARAALESQLRQAKTELEETTAEAEEEIQALTAHRDEIQRKFDALRNSCTVITDLEEQLNQLTEDNAELNNQNFYLSKQLDEASGANDEIVQLRSEVDHLRREITEREMQLTSQKQAQLSAPDLQTMEALKTTCTMLEEQVLDLEALNDELLEKERQWEAWRSVLGDEKSQFECRVRELQRMLDTEKQSRARADQRITESRQVVELAVKEHKAEILALQQALKEQKLKAESLSDKLNDLEKKHAMLEMNARSLQQKLETERELKQRLLEEQAKLQQQMDLQKNHIFRLTQGLQEALDRADLLKTERSDLEYQLENIQVLYSHEKVKMEGTISQQTKLIDFLQAKMDQPAKKKKGLFSRRKEDPALPTQVPLQYNELKLALEKEKARCAELEEALQKTRIELRSAREEAAHRKATDHPHPSTPATARQQIAMSAIVRSPEHQPSAMSLLAPPSSRRKESSTPEEFSRRLKERMHHNIPHRFNVGLNMRATKCAVCLDTVHFGRQASKCLECQVMCHPKCSTCLPATCGLPAEYATHFTEAFCRDKMNSPGLQSKEPGSSLHLEGWMKVPRNNKRGQQGWDRKYIVLEGSKVLIYDNEAREAGQRPVEEFELCLPDGDVSIHGAVGASELANTAKADVPYILKMESHPHTTCWPGRTLYLLAPSFPDKQRWVTALESVVAGGRVSREKAEADAARDHTSSEHQPVWVEKLLGNSLLKLEGDDRLDMNCTLPFSDQVVLVGTEEGLYALNVLKNSLTHIPGIGAVFQIYIIKDLEKLLMIAGEERALCLVDVKKVKQSLAQSHLPAQPDVSPNIFEAVKGCHLFAAGKIENSLCICAAMPSKVVILRYNDNLSKYCIRKEIETSEPCSCIHFTNYSILIGTNKFYEIDMKQYTLDEFLDKNDHSLAPAVFASSSNSFPVSIVQANSAGQREEYLLCFHEFGVFVDSYGRRSRTDDLKWSRLPLAFAYREPYLFVTHFNSLEVIEIQARSSLGSPARAYLEIPNPRYLGPAISSGAIYLASSYQDKLRVICCKGNLVKESGTEQHRVPSTSRSSPNKRGPPTYNEHITKRVASSPAPPEGPSHPREPSTPHRYRDREGRTELRRDKSPGRPLEREKSPGRMLSTRRERSPGRLFEDSSRGRLPAGAVRTPLSQVNKVWDQSSV

Target ORF information:

RefSeq Version	XM_006530139.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus citron (Cit), transcript variant X13, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006530139.4

ORF Insert Sequence:

ATGTTGAAGT TCAAGTATGG TGTGCGGAAC CCGCCGGAGG CCAGTGCCTC CGAGCCCATT 
GCCAGTCGGG CCTCCAGGCT AAATCTCTTC TTCCAGGGGA AACCGCCCCT CATGACTCAA 
CAGCAGATGT CTGCTCTTTC CCGGGAAGGG ATGCTAGACG CCCTCTTCGC TCTCTTTGAA 
GAGTGCAGCC AACCCGCCCT GATGAAGATG AAGCACGTGA GCAGCTTTGT CCAGAAGTAT 
TCCGACACCA TAGCCGAGTT GCGGGAGCTG CAGCCGTCGG CGAGAGACTT CGAAGTTCGA 
AGCCTTGTGG GCTGTGGTCA CTTCGCTGAA GTGCAGGTGG TTAGAGAGAA GGCGACCGGG 
GACGTCTATG CCATGAAAAT CATGAAGAAG AAGGCTTTGC TGGCCCAGGA ACAGGTTTCA 
TTTTTCGAGG AGGAGAGGAA CATATTATCT CGGAGCACGA GTCCTTGGAT CCCCCAGTTA 
CAGTACGCCT TTCAGGACAA AAATAACCTT TACCTGGTCA TGGAATATCA GCCTGGAGGG 
GATTTGCTGT CGCTTCTGAA CAGATACGAG GACCAATTAG ATGAGAGCAT GATCCAGTTT 
TACCTTGCTG AGCTGATTTT GGCTGTCCAC AGCGTGCACC AGATGGGATA TGTGCATCGA 
GACATCAAGC CCGAGAACAT CCTCATCGAC CGGACGGGAC ACATCAAGCT GGTGGATTTT 
GGATCAGCCG CTAAGATGAA TTCAAATAAG GTGGATGCCA AACTCCCCAT TGGGACCCCG 
GATTACATGG CTCCGGAAGT GTTGACCGTG ATGAACGAGG ACCGAAGGGG CACATACGGC 
TTGGACTGTG ACTGGTGGTC TGTCGGAGTT GTTGCTTATG AGATGGTTTA TGGGAAGACC 
CCATTCACAG AGGGAACCTC CGCCCGGACC TTCAACAACA TCATGAACTT CCAGCGGTTT 
TTGAAGTTCC CAGATGACCC CAAAGTTAGC AGTGAGCTCC TTGATCTGCT TCAGAGTCTG 
CTGTGTGTCC AGAAAGAGAG ACTGAAGTTC GAGGGTCTCT GCTGCCACCC TTTCTTTGCC 
AGAACGGACT GGAACAACAT CCGTAACTCT CCTCCCCCCT TCGTCCCCAC CCTCAAGTCT 
GACGATGACA CCTCCAATTT TGATGAACCA GAGAAGAATT CGTGGGTTTC ATCCTCTGTG 
TGCCAGCTGA GCCCCTCGGG CTTCTCAGGC GAAGAGCTGC CGTTTGTGGG ATTTTCGTAC 
AGCAAGGCAC TGGGGTATCT TGGTAGATCT GAGTCTGTCG TGTCGAGTCT GGACTCCCCT 
GCCAAGGTTA GCTCCATGGA AAAGAAACTT CTCATCAAAA GCAAAGAGCT CCAAGACTCC 
CAGGACAAGT GTCACAAGAT GGAGCAGGAA ATGACCCGGT TACATCGCAG AGTGTCAGAG 
GTGGAGGCTG TGCTTAGTCA GAAGGAGGTG GAGCTGAAGG CCTCTGAGAC TCAGAGATCC 
CTCCTGGAGC AGGACCTTGC TACCTACATC ACAGAATGCA GTAGCTTAAA GCGAAGTTTG 
GAGCAAGCGC GGATGGAGGT GTCCCAGGAG GATGACAAAG CTCTGCAGCT TCTCCACGAC 
ATCCGAGAGC AGAGCCGGAA GCTCCAGGAG ATCAAGGAGC AGGAGTACCA GGCTCAGGTG 
GAGGAGATGA GGCTGATGAT GAATCAGCTG GAAGAAGACC TGGTGTCAGC CCGCAGACGC 
AGCGATCTCT ACGAGTCTGA GCTGAGGGAG TCTCGGCTTG CCGCCGAGGA ATTCAAGCGG 
AAGGCAAACG AATGTCAGCA CAAACTGATG AAGGTAGTCA GCCACCCTCC AAGAGGGGAC 
TCCGGGGGCA CGGCCCTGGA TGATCTTCAC AAGATGCAAG GCCACGCTGG CTTAACGAGT 
GCTAAGGACC AAGGGAAGCC TGAAGTGGGA GAATATTCCA AACTGGAGAA GATCAATGCT 
GAGCAGCAGC TGAAGATCCA GGAGCTCCAG GAGAAGCTGG AGAAGGCTGT AAAAGCCAGC 
ACAGAGGCCA CCGAGCTCCT GCAGAATATC CGCCAGGCAA AGGAGCGAGC AGAGCGGGAG 
CTAGAGAAGC TACACAACCG GGAAGACTCC TCCGAGGGCA TCAAAAAGAA GCTGGTGGAA 
GCCGAGGAGC TGGAGGAGAA ACATCGGGAG GCTCAGGTCT CAGCTCAACA TCTAGAAGTA 
CACTTGAAAC AGAAAGAACA GCACTACGAG GAAAAGATCA AGGTATTGGA CAATCAGATA 
AAGAAGGACC TGGCCGACAA GGAGAGCCTG GAGAACATGA TGCAGAGACA CGAGGAGGAG 
GCCCACGAGA AGGGCAAGAT CCTCAGCGAG CAGAAGGCGA TGATCAACGC GATGGATTCC 
AAGATCCGAT CCCTGGAGCA GAGGATCGTG GAGCTGTCGG AAGCCAACAA GCTTGCGGCA 
AACAGCAGTC TCTTCACCCA GAGGAACATG AAGGCCCAGG AAGAGATGAT CTCAGAACTC 
AGGCAGCAGA AATTTTACCT GGAGACGCAG GCCGGAAAGC TGGAGGCCCA GAACCGAAAG 
CTGGAAGAGC AACTGGAGAA GATCAGCCAC CAAGATCACA GTGACAAGAG TCGGCTGCTG 
GAGCTGGAAA CAAGGCTGAG GGAGGTCAGC CTGGAGCACG AGGAGCAGAA GCTGGAGCTG 
AAGCGGCAGC TCACGGAGCT GCAGCTGTCC CTGCAGGAGC GCGAGTCCCA GCTGACGGCC 
CTGCAGGCCG CCCGGGCAGC GCTGGAGAGC CAGCTCCGCC AGGCGAAGAC AGAGCTGGAG 
GAGACAACCG CGGAAGCGGA GGAGGAGATC CAGGCGCTCA CGGCACATCG AGATGAGATC 
CAGCGCAAAT TCGATGCCCT TCGCAACAGC TGCACGGTCA TCACCGACCT GGAGGAGCAG 
CTGAACCAGC TCACCGAGGA CAACGCCGAG CTCAACAACC AAAACTTCTA CCTGTCCAAA 
CAACTCGATG AGGCTTCCGG GGCCAATGAC GAGATTGTGC AGCTGCGGAG TGAGGTGGAC 
CATCTCCGCC GTGAGATCAC GGAGCGGGAG ATGCAGCTCA CCAGCCAGAA GCAAGCACAA 
CTCAGTGCTC CTGATTTGCA GACGATGGAG GCTCTGAAGA CGACATGCAC CATGCTGGAG 
GAGCAGGTCC TGGACCTGGA GGCCCTGAAC GACGAGCTGC TGGAGAAGGA GCGCCAGTGG 
GAGGCCTGGC GGAGCGTCCT CGGCGACGAG AAGTCCCAGT TCGAGTGTCG AGTTCGAGAG 
CTACAGAGGA TGCTGGACAC CGAGAAGCAG AGCAGGGCTA GGGCCGATCA GCGGATCACC 
GAGTCGCGCC AGGTGGTGGA GTTGGCGGTG AAGGAACACA AGGCCGAGAT TCTTGCTCTG 
CAGCAGGCTC TCAAGGAGCA GAAGCTCAAA GCCGAGAGCC TGTCGGACAA GCTCAACGAC 
CTGGAGAAGA AGCACGCCAT GCTGGAGATG AACGCCCGGA GCTTACAGCA GAAACTAGAG 
ACAGAGCGGG AGCTCAAACA GAGGCTTCTG GAGGAGCAAG CCAAATTGCA GCAGCAGATG 
GACCTGCAGA AGAACCACAT CTTCAGACTG ACGCAAGGGC TGCAGGAGGC GCTGGACCGG 
GCGGATCTGC TGAAGACAGA AAGGAGCGAC CTGGAGTACC AGCTGGAAAA CATTCAGGTT 
CTCTACTCTC ACGAGAAAGT GAAAATGGAA GGCACAATCT CTCAGCAAAC CAAACTCATT 
GATTTCCTGC AAGCCAAAAT GGACCAGCCT GCTAAAAAGA AAAAGGGTTT ATTTAGTCGA 
CGGAAAGAGG ACCCTGCTTT GCCCACACAG GTTCCTCTGC AGTACAATGA GCTGAAGCTA 
GCCCTGGAAA AGGAGAAAGC CCGATGCGCG GAGCTGGAGG AGGCCCTTCA GAAGACCCGC 
ATCGAACTCC GCTCTGCGCG GGAGGAAGCT GCCCACCGCA AAGCCACGGA CCACCCGCAC 
CCGTCTACGC CAGCCACTGC GAGGCAGCAG ATCGCCATGT CTGCCATTGT GCGGTCGCCC 
GAGCACCAGC CCAGTGCCAT GAGCCTGCTT GCCCCACCAT CCAGCCGCAG AAAGGAGTCG 
TCAACTCCAG AGGAATTCAG CCGGCGTCTG AAAGAGCGCA TGCACCACAA CATCCCTCAC 
CGGTTTAATG TGGGCCTGAA CATGAGAGCC ACCAAGTGCG CCGTGTGTCT GGATACTGTG 
CACTTTGGAC GCCAGGCATC CAAATGCCTC GAATGTCAAG TGATGTGTCA CCCCAAATGC 
TCCACCTGCT TGCCCGCTAC CTGTGGCCTG CCAGCTGAAT ATGCCACACA CTTCACTGAG 
GCCTTCTGCC GGGACAAAAT GAACTCCCCG GGGCTCCAGA GCAAGGAGCC TGGCAGCAGC 
TTGCACCTGG AAGGGTGGAT GAAAGTGCCC AGAAATAACA AACGGGGACA GCAAGGCTGG 
GACAGGAAGT ACATTGTTCT GGAGGGGTCA AAAGTCCTCA TCTATGACAA TGAAGCCAGA 
GAAGCTGGAC AGAGGCCGGT GGAAGAATTT GAGCTGTGCC TTCCTGACGG GGACGTATCT 
ATTCATGGTG CCGTTGGTGC TTCAGAACTT GCAAATACCG CCAAAGCAGA TGTCCCATAC 
ATCCTGAAGA TGGAGTCTCA CCCACACACC ACCTGCTGGC CTGGGAGAAC CCTCTACTTG 
CTAGCACCCA GCTTCCCCGA CAAGCAGCGC TGGGTCACCG CCTTAGAATC TGTCGTCGCA 
GGTGGGAGAG TTTCTAGGGA AAAGGCCGAA GCCGATGCTG CACGTGACCA TACTTCGAGT 
GAGCATCAGC CAGTGTGGGT TGAGAAATTA CTTGGAAACT CTCTGCTGAA ACTGGAGGGC 
GATGACCGGC TTGACATGAA CTGCACCCTG CCCTTCAGTG ACCAGGTAGT GCTGGTGGGC 
ACGGAGGAAG GCCTCTACGC CCTGAATGTC TTGAAAAACT CCTTAACCCA CATCCCAGGG 
ATTGGCGCAG TCTTCCAAAT TTACATCATC AAGGACCTGG AGAAGCTGCT CATGATAGCA 
GGGGAAGAGC GGGCTCTGTG CCTGGTGGAC GTGAAGAAGG TGAAGCAGTC CCTGGCGCAG 
TCACACCTGC CTGCCCAGCC CGACGTCTCC CCCAACATAT TCGAAGCCGT CAAAGGCTGC 
CACTTGTTCG CTGCTGGCAA GATCGAGAAC AGCCTGTGCA TCTGCGCCGC TATGCCAAGC 
AAAGTCGTCA TCCTCCGCTA CAATGACAAC CTCAGCAAGT ACTGCATCCG CAAGGAGATC 
GAGACCTCAG AGCCCTGCAG CTGTATCCAC TTCACCAACT ACAGCATCCT CATTGGGACC 
AACAAATTCT ATGAGATCGA CATGAAGCAG TACACGCTTG ACGAGTTCCT GGACAAGAAC 
GACCATTCCT TGGCACCTGC TGTGTTCGCC TCCTCGTCCA ACAGCTTCCC TGTCTCCATT 
GTGCAGGCGA ACAGCGCCGG GCAGCGAGAA GAATACCTGC TGTGCTTCCA CGAATTTGGG 
GTGTTCGTGG ATTCTTACGG AAGACGTAGC CGCACAGATG ATCTCAAGTG GAGTCGCTTA 
CCTCTGGCCT TCGCCTACAG AGAACCTTAT CTGTTTGTGA CTCACTTCAA CTCCCTGGAA 
GTCATTGAGA TCCAGGCCAG ATCCTCACTG GGGAGCCCTG CCCGAGCATA TCTGGAAATT 
CCAAACCCTC GCTACCTGGG CCCCGCGATT TCCTCCGGAG CGATTTACCT GGCCTCCTCA 
TACCAGGACA AGTTAAGGGT CATATGCTGC AAAGGAAACC TCGTGAAGGA GTCAGGCACT 
GAGCAGCACC GGGTGCCCTC CACCTCCCGC AGCAGCCCCA ACAAGCGAGG CCCACCAACA 
TACAACGAGC ACATCACCAA ACGCGTGGCC TCCAGCCCGG CGCCACCGGA AGGCCCCAGC 
CACCCCCGAG AGCCAAGCAC ACCGCACCGC TACCGAGACA GAGAGGGTCG GACAGAGCTG 
CGCAGGGACA AGTCTCCAGG CCGCCCTCTG GAGCGGGAGA AGTCCCCAGG CCGAATGCTC 
AGCACTAGGA GAGAGCGGTC CCCAGGGAGA CTGTTTGAAG ACAGCAGCAG GGGCCGGCTG 
CCTGCAGGAG CAGTGAGGAC CCCACTGTCC CAGGTTAACA AGGTCTGGGA CCAGTCTTCA 
GTATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cit ( XM_006530140.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006530203.1 Mus musculus citron (Cit), transcript variant X14, mRNA.

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CloneID	OMu35781
Clone ID Related Accession (Same CDS sequence)	XM_006530140.4 , XM_006530140.3
Accession Version	XM_006530140.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6213bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	citron Rho-interacting kinase isoform X10
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000071.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006530140.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTGAAGT TCAAGTATGG TGTGCGGAAC CCGCCGGAGG CCAGTGCCTC CGAGCCCATT 
GCCAGTCGGG CCTCCAGGCT AAATCTCTTC TTCCAGGGGA AACCGCCCCT CATGACTCAA 
CAGCAGATGT CTGCTCTTTC CCGGGAAGGG ATGCTAGACG CCCTCTTCGC TCTCTTTGAA 
GAGTGCAGCC AACCCGCCCT GATGAAGATG AAGCACGTGA GCAGCTTTGT CCAGAAGTAT 
TCCGACACCA TAGCCGAGTT GCGGGAGCTG CAGCCGTCGG CGAGAGACTT CGAAGTTCGA 
AGCCTTGTGG GCTGTGGTCA CTTCGCTGAA GTGCAGGTGG TTAGAGAGAA GGCGACCGGG 
GACGTCTATG CCATGAAAAT CATGAAGAAG AAGGCTTTGC TGGCCCAGGA ACAGGTTTCA 
TTTTTCGAGG AGGAGAGGAA CATATTATCT CGGAGCACGA GTCCTTGGAT CCCCCAGTTA 
CAGTACGCCT TTCAGGACAA AAATAACCTT TACCTGGTCA TGGAATATCA GCCTGGAGGG 
GATTTGCTGT CGCTTCTGAA CAGATACGAG GACCAATTAG ATGAGAGCAT GATCCAGTTT 
TACCTTGCTG AGCTGATTTT GGCTGTCCAC AGCGTGCACC AGATGGGATA TGTGCATCGA 
GACATCAAGC CCGAGAACAT CCTCATCGAC CGGACGGGAC ACATCAAGCT GGTGGATTTT 
GGATCAGCCG CTAAGATGAA TTCAAATAAG GTGGATGCCA AACTCCCCAT TGGGACCCCG 
GATTACATGG CTCCGGAAGT GTTGACCGTG ATGAACGAGG ACCGAAGGGG CACATACGGC 
TTGGACTGTG ACTGGTGGTC TGTCGGAGTT GTTGCTTATG AGATGGTTTA TGGGAAGACC 
CCATTCACAG AGGGAACCTC CGCCCGGACC TTCAACAACA TCATGAACTT CCAGCGGTTT 
TTGAAGTTCC CAGATGACCC CAAAGTTAGC AGTGAGCTCC TTGATCTGCT TCAGAGTCTG 
CTGTGTGTCC AGAAAGAGAG ACTGAAGTTC GAGGGTCTCT GCTGCCACCC TTTCTTTGCC 
AGAACGGACT GGAACAACAT CCGTAACTCT CCTCCCCCCT TCGTCCCCAC CCTCAAGTCT 
GACGATGACA CCTCCAATTT TGATGAACCA GAGAAGAATT CGTGGGTTTC ATCCTCTGTG 
TGCCAGCTGA GCCCCTCGGG CTTCTCAGGC GAAGAGCTGC CGTTTGTGGG ATTTTCGTAC 
AGCAAGGCAC TGGGGTATCT TGGTAGATCT GAGTCTGTCG TGTCGAGTCT GGACTCCCCT 
GCCAAGGTTA GCTCCATGGA AAAGAAACTT CTCATCAAAA GCAAAGAGCT CCAAGACTCC 
CAGGACAAGT GTCACAAGAT GGAGCAGGAA ATGACCCGGT TACATCGCAG AGTGTCAGAG 
GTGGAGGCTG TGCTTAGTCA GAAGGAGGTG GAGCTGAAGG CCTCTGAGAC TCAGAGATCC 
CTCCTGGAGC AGGACCTTGC TACCTACATC ACAGAATGCA GTAGCTTAAA GCGAAGTTTG 
GAGCAAGCGC GGATGGAGGT GTCCCAGGAG GATGACAAAG CTCTGCAGCT TCTCCACGAC 
ATCCGAGAGC AGAGCCGGAA GCTCCAGGAG ATCAAGGAGC AGGAGTACCA GGCTCAGGTG 
GAGGAGATGA GGCTGATGAT GAATCAGCTG GAAGAAGACC TGGTGTCAGC CCGCAGACGC 
AGCGATCTCT ACGAGTCTGA GCTGAGGGAG TCTCGGCTTG CCGCCGAGGA ATTCAAGCGG 
AAGGCAAACG AATGTCAGCA CAAACTGATG AAGGCTAAGG ACCAAGGGAA GCCTGAAGTG 
GGAGAATATT CCAAACTGGA GAAGATCAAT GCTGAGCAGC AGCTGAAGAT CCAGGAGCTC 
CAGGAGAAGC TGGAGAAGGC TGTAAAAGCC AGCACAGAGG CCACCGAGCT CCTGCAGAAT 
ATCCGCCAGG CAAAGGAGCG AGCAGAGCGG GAGCTAGAGA AGCTACACAA CCGGGAAGAC 
TCCTCCGAGG GCATCAAAAA GAAGCTGGTG GAAGCCGAGG AACGCCGCCA CTCCCTGGAG 
AACAAGGTAA AGAGACTAGA GACCATGGAG CGTAGAGAGA ACAGACTGAA GGATGACATC 
CAGACAAAGT CCGAACAGAT CCAGCAGATG GCTGATAAAA TTCTGGAGCT GGAGGAGAAA 
CATCGGGAGG CTCAGGTCTC AGCTCAACAT CTAGAAGTAC ACTTGAAACA GAAAGAACAG 
CACTACGAGG AAAAGATCAA GGTATTGGAC AATCAGATAA AGAAGGACCT GGCCGACAAG 
GAGAGCCTGG AGAACATGAT GCAGAGACAC GAGGAGGAGG CCCACGAGAA GGGCAAGATC 
CTCAGCGAGC AGAAGGCGAT GATCAACGCG ATGGATTCCA AGATCCGATC CCTGGAGCAG 
AGGATCGTGG AGCTGTCGGA AGCCAACAAG CTTGCGGCAA ACAGCAGTCT CTTCACCCAG 
AGGAACATGA AGGCCCAGGA AGAGATGATC TCAGAACTCA GGCAGCAGAA ATTTTACCTG 
GAGACGCAGG CCGGAAAGCT GGAGGCCCAG AACCGAAAGC TGGAAGAGCA ACTGGAGAAG 
ATCAGCCACC AAGATCACAG TGACAAGAGT CGGCTGCTGG AGCTGGAAAC AAGGCTGAGG 
GAGGTCAGCC TGGAGCACGA GGAGCAGAAG CTGGAGCTGA AGCGGCAGCT CACGGAGCTG 
CAGCTGTCCC TGCAGGAGCG CGAGTCCCAG CTGACGGCCC TGCAGGCCGC CCGGGCAGCG 
CTGGAGAGCC AGCTCCGCCA GGCGAAGACA GAGCTGGAGG AGACAACCGC GGAAGCGGAG 
GAGGAGATCC AGGCGCTCAC GGCACATCGA GATGAGATCC AGCGCAAATT CGATGCCCTT 
CGCAACAGCT GCACGGTCAT CACCGACCTG GAGGAGCAGC TGAACCAGCT CACCGAGGAC 
AACGCCGAGC TCAACAACCA AAACTTCTAC CTGTCCAAAC AACTCGATGA GGCTTCCGGG 
GCCAATGACG AGATTGTGCA GCTGCGGAGT GAGGTGGACC ATCTCCGCCG TGAGATCACG 
GAGCGGGAGA TGCAGCTCAC CAGCCAGAAG CAAACGATGG AGGCTCTGAA GACGACATGC 
ACCATGCTGG AGGAGCAGGT CCTGGACCTG GAGGCCCTGA ACGACGAGCT GCTGGAGAAG 
GAGCGCCAGT GGGAGGCCTG GCGGAGCGTC CTCGGCGACG AGAAGTCCCA GTTCGAGTGT 
CGAGTTCGAG AGCTACAGAG GATGCTGGAC ACCGAGAAGC AGAGCAGGGC TAGGGCCGAT 
CAGCGGATCA CCGAGTCGCG CCAGGTGGTG GAGTTGGCGG TGAAGGAACA CAAGGCCGAG 
ATTCTTGCTC TGCAGCAGGC TCTCAAGGAG CAGAAGCTCA AAGCCGAGAG CCTGTCGGAC 
AAGCTCAACG ACCTGGAGAA GAAGCACGCC ATGCTGGAGA TGAACGCCCG GAGCTTACAG 
CAGAAACTAG AGACAGAGCG GGAGCTCAAA CAGAGGCTTC TGGAGGAGCA AGCCAAATTG 
CAGCAGCAGA TGGACCTGCA GAAGAACCAC ATCTTCAGAC TGACGCAAGG GCTGCAGGAG 
GCGCTGGACC GGGCGGATCT GCTGAAGACA GAAAGGAGCG ACCTGGAGTA CCAGCTGGAA 
AACATTCAGG TTCTCTACTC TCACGAGAAA GTGAAAATGG AAGGCACAAT CTCTCAGCAA 
ACCAAACTCA TTGATTTCCT GCAAGCCAAA ATGGACCAGC CTGCTAAAAA GAAAAAGGGT 
TTATTTAGTC GACGGAAAGA GGACCCTGCT TTGCCCACAC AGGTTCCTCT GCAGTACAAT 
GAGCTGAAGC TAGCCCTGGA AAAGGAGAAA GCCCGATGCG CGGAGCTGGA GGAGGCCCTT 
CAGAAGACCC GCATCGAACT CCGCTCTGCG CGGGAGGAAG CTGCCCACCG CAAAGCCACG 
GACCACCCGC ACCCGTCTAC GCCAGCCACT GCGAGGCAGC AGATCGCCAT GTCTGCCATT 
GTGCGGTCGC CCGAGCACCA GCCCAGTGCC ATGAGCCTGC TTGCCCCACC ATCCAGCCGC 
AGAAAGGAGT CGTCAACTCC AGAGGAATTC AGCCGGCGTC TGAAAGAGCG CATGCACCAC 
AACATCCCTC ACCGGTTTAA TGTGGGCCTG AACATGAGAG CCACCAAGTG CGCCGTGTGT 
CTGGATACTG TGCACTTTGG ACGCCAGGCA TCCAAATGCC TCGAATGTCA AGTGATGTGT 
CACCCCAAAT GCTCCACCTG CTTGCCCGCT ACCTGTGGCC TGCCAGCTGA ATATGCCACA 
CACTTCACTG AGGCCTTCTG CCGGGACAAA ATGAACTCCC CGGGGCTCCA GAGCAAGGAG 
CCTGGCAGCA GCTTGCACCT GGAAGGGTGG ATGAAAGTGC CCAGAAATAA CAAACGGGGA 
CAGCAAGGCT GGGACAGGAA GTACATTGTT CTGGAGGGGT CAAAAGTCCT CATCTATGAC 
AATGAAGCCA GAGAAGCTGG ACAGAGGCCG GTGGAAGAAT TTGAGCTGTG CCTTCCTGAC 
GGGGACGTAT CTATTCATGG TGCCGTTGGT GCTTCAGAAC TTGCAAATAC CGCCAAAGCA 
GATGTCCCAT ACATCCTGAA GATGGAGTCT CACCCACACA CCACCTGCTG GCCTGGGAGA 
ACCCTCTACT TGCTAGCACC CAGCTTCCCC GACAAGCAGC GCTGGGTCAC CGCCTTAGAA 
TCTGTCGTCG CAGGTGGGAG AGTTTCTAGG GAAAAGGCCG AAGCCGATGC TAAATTACTT 
GGAAACTCTC TGCTGAAACT GGAGGGCGAT GACCGGCTTG ACATGAACTG CACCCTGCCC 
TTCAGTGACC AGGTAGTGCT GGTGGGCACG GAGGAAGGCC TCTACGCCCT GAATGTCTTG 
AAAAACTCCT TAACCCACAT CCCAGGGATT GGCGCAGTCT TCCAAATTTA CATCATCAAG 
GACCTGGAGA AGCTGCTCAT GATAGCAGGG GAAGAGCGGG CTCTGTGCCT GGTGGACGTG 
AAGAAGGTGA AGCAGTCCCT GGCGCAGTCA CACCTGCCTG CCCAGCCCGA CGTCTCCCCC 
AACATATTCG AAGCCGTCAA AGGCTGCCAC TTGTTCGCTG CTGGCAAGAT CGAGAACAGC 
CTGTGCATCT GCGCCGCTAT GCCAAGCAAA GTCGTCATCC TCCGCTACAA TGACAACCTC 
AGCAAGTACT GCATCCGCAA GGAGATCGAG ACCTCAGAGC CCTGCAGCTG TATCCACTTC 
ACCAACTACA GCATCCTCAT TGGGACCAAC AAATTCTATG AGATCGACAT GAAGCAGTAC 
ACGCTTGACG AGTTCCTGGA CAAGAACGAC CATTCCTTGG CACCTGCTGT GTTCGCCTCC 
TCGTCCAACA GCTTCCCTGT CTCCATTGTG CAGGCGAACA GCGCCGGGCA GCGAGAAGAA 
TACCTGCTGT GCTTCCACGA ATTTGGGGTG TTCGTGGATT CTTACGGAAG ACGTAGCCGC 
ACAGATGATC TCAAGTGGAG TCGCTTACCT CTGGCCTTCG CCTACAGAGA ACCTTATCTG 
TTTGTGACTC ACTTCAACTC CCTGGAAGTC ATTGAGATCC AGGCCAGATC CTCACTGGGG 
AGCCCTGCCC GAGCATATCT GGAAATTCCA AACCCTCGCT ACCTGGGCCC CGCGATTTCC 
TCCGGAGCGA TTTACCTGGC CTCCTCATAC CAGGACAAGT TAAGGGTCAT ATGCTGCAAA 
GGAAACCTCG TGAAGGAGTC AGGCACTGAG CAGCACCGGG TGCCCTCCAC CTCCCGCAGC 
AGCCCCAACA AGCGAGGCCC ACCAACATAC AACGAGCACA TCACCAAACG CGTGGCCTCC 
AGCCCGGCGC CACCGGAAGG CCCCAGCCAC CCCCGAGAGC CAAGCACACC GCACCGCTAC 
CGAGACAGAG AGGGTCGGAC AGAGCTGCGC AGGGACAAGT CTCCAGGCCG CCCTCTGGAG 
CGGGAGAAGT CCCCAGGCCG AATGCTCAGC ACTAGGAGAG AGCGGTCCCC AGGGAGACTG 
TTTGAAGACA GCAGCAGGGG CCGGCTGCCT GCAGGAGCAG TGAGGACCCC ACTGTCCCAG 
GTTAACAAGG TCTGGGACCA GTCTTCAGTA TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006530203.1
CDS	157..6369
Translation	MLKFKYGVRNPPEASASEPIASRASRLNLFFQGKPPLMTQQQMSALSREGMLDALFALFEECSQPALMKMKHVSSFVQKYSDTIAELRELQPSARDFEVRSLVGCGHFAEVQVVREKATGDVYAMKIMKKKALLAQEQVSFFEEERNILSRSTSPWIPQLQYAFQDKNNLYLVMEYQPGGDLLSLLNRYEDQLDESMIQFYLAELILAVHSVHQMGYVHRDIKPENILIDRTGHIKLVDFGSAAKMNSNKVDAKLPIGTPDYMAPEVLTVMNEDRRGTYGLDCDWWSVGVVAYEMVYGKTPFTEGTSARTFNNIMNFQRFLKFPDDPKVSSELLDLLQSLLCVQKERLKFEGLCCHPFFARTDWNNIRNSPPPFVPTLKSDDDTSNFDEPEKNSWVSSSVCQLSPSGFSGEELPFVGFSYSKALGYLGRSESVVSSLDSPAKVSSMEKKLLIKSKELQDSQDKCHKMEQEMTRLHRRVSEVEAVLSQKEVELKASETQRSLLEQDLATYITECSSLKRSLEQARMEVSQEDDKALQLLHDIREQSRKLQEIKEQEYQAQVEEMRLMMNQLEEDLVSARRRSDLYESELRESRLAAEEFKRKANECQHKLMKAKDQGKPEVGEYSKLEKINAEQQLKIQELQEKLEKAVKASTEATELLQNIRQAKERAERELEKLHNREDSSEGIKKKLVEAEERRHSLENKVKRLETMERRENRLKDDIQTKSEQIQQMADKILELEEKHREAQVSAQHLEVHLKQKEQHYEEKIKVLDNQIKKDLADKESLENMMQRHEEEAHEKGKILSEQKAMINAMDSKIRSLEQRIVELSEANKLAANSSLFTQRNMKAQEEMISELRQQKFYLETQAGKLEAQNRKLEEQLEKISHQDHSDKSRLLELETRLREVSLEHEEQKLELKRQLTELQLSLQERESQLTALQAARAALESQLRQAKTELEETTAEAEEEIQALTAHRDEIQRKFDALRNSCTVITDLEEQLNQLTEDNAELNNQNFYLSKQLDEASGANDEIVQLRSEVDHLRREITEREMQLTSQKQTMEALKTTCTMLEEQVLDLEALNDELLEKERQWEAWRSVLGDEKSQFECRVRELQRMLDTEKQSRARADQRITESRQVVELAVKEHKAEILALQQALKEQKLKAESLSDKLNDLEKKHAMLEMNARSLQQKLETERELKQRLLEEQAKLQQQMDLQKNHIFRLTQGLQEALDRADLLKTERSDLEYQLENIQVLYSHEKVKMEGTISQQTKLIDFLQAKMDQPAKKKKGLFSRRKEDPALPTQVPLQYNELKLALEKEKARCAELEEALQKTRIELRSAREEAAHRKATDHPHPSTPATARQQIAMSAIVRSPEHQPSAMSLLAPPSSRRKESSTPEEFSRRLKERMHHNIPHRFNVGLNMRATKCAVCLDTVHFGRQASKCLECQVMCHPKCSTCLPATCGLPAEYATHFTEAFCRDKMNSPGLQSKEPGSSLHLEGWMKVPRNNKRGQQGWDRKYIVLEGSKVLIYDNEAREAGQRPVEEFELCLPDGDVSIHGAVGASELANTAKADVPYILKMESHPHTTCWPGRTLYLLAPSFPDKQRWVTALESVVAGGRVSREKAEADAKLLGNSLLKLEGDDRLDMNCTLPFSDQVVLVGTEEGLYALNVLKNSLTHIPGIGAVFQIYIIKDLEKLLMIAGEERALCLVDVKKVKQSLAQSHLPAQPDVSPNIFEAVKGCHLFAAGKIENSLCICAAMPSKVVILRYNDNLSKYCIRKEIETSEPCSCIHFTNYSILIGTNKFYEIDMKQYTLDEFLDKNDHSLAPAVFASSSNSFPVSIVQANSAGQREEYLLCFHEFGVFVDSYGRRSRTDDLKWSRLPLAFAYREPYLFVTHFNSLEVIEIQARSSLGSPARAYLEIPNPRYLGPAISSGAIYLASSYQDKLRVICCKGNLVKESGTEQHRVPSTSRSSPNKRGPPTYNEHITKRVASSPAPPEGPSHPREPSTPHRYRDREGRTELRRDKSPGRPLEREKSPGRMLSTRRERSPGRLFEDSSRGRLPAGAVRTPLSQVNKVWDQSSV

Target ORF information:

RefSeq Version	XM_006530140.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus citron (Cit), transcript variant X14, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006530140.4

ORF Insert Sequence:

ATGTTGAAGT TCAAGTATGG TGTGCGGAAC CCGCCGGAGG CCAGTGCCTC CGAGCCCATT 
GCCAGTCGGG CCTCCAGGCT AAATCTCTTC TTCCAGGGGA AACCGCCCCT CATGACTCAA 
CAGCAGATGT CTGCTCTTTC CCGGGAAGGG ATGCTAGACG CCCTCTTCGC TCTCTTTGAA 
GAGTGCAGCC AACCCGCCCT GATGAAGATG AAGCACGTGA GCAGCTTTGT CCAGAAGTAT 
TCCGACACCA TAGCCGAGTT GCGGGAGCTG CAGCCGTCGG CGAGAGACTT CGAAGTTCGA 
AGCCTTGTGG GCTGTGGTCA CTTCGCTGAA GTGCAGGTGG TTAGAGAGAA GGCGACCGGG 
GACGTCTATG CCATGAAAAT CATGAAGAAG AAGGCTTTGC TGGCCCAGGA ACAGGTTTCA 
TTTTTCGAGG AGGAGAGGAA CATATTATCT CGGAGCACGA GTCCTTGGAT CCCCCAGTTA 
CAGTACGCCT TTCAGGACAA AAATAACCTT TACCTGGTCA TGGAATATCA GCCTGGAGGG 
GATTTGCTGT CGCTTCTGAA CAGATACGAG GACCAATTAG ATGAGAGCAT GATCCAGTTT 
TACCTTGCTG AGCTGATTTT GGCTGTCCAC AGCGTGCACC AGATGGGATA TGTGCATCGA 
GACATCAAGC CCGAGAACAT CCTCATCGAC CGGACGGGAC ACATCAAGCT GGTGGATTTT 
GGATCAGCCG CTAAGATGAA TTCAAATAAG GTGGATGCCA AACTCCCCAT TGGGACCCCG 
GATTACATGG CTCCGGAAGT GTTGACCGTG ATGAACGAGG ACCGAAGGGG CACATACGGC 
TTGGACTGTG ACTGGTGGTC TGTCGGAGTT GTTGCTTATG AGATGGTTTA TGGGAAGACC 
CCATTCACAG AGGGAACCTC CGCCCGGACC TTCAACAACA TCATGAACTT CCAGCGGTTT 
TTGAAGTTCC CAGATGACCC CAAAGTTAGC AGTGAGCTCC TTGATCTGCT TCAGAGTCTG 
CTGTGTGTCC AGAAAGAGAG ACTGAAGTTC GAGGGTCTCT GCTGCCACCC TTTCTTTGCC 
AGAACGGACT GGAACAACAT CCGTAACTCT CCTCCCCCCT TCGTCCCCAC CCTCAAGTCT 
GACGATGACA CCTCCAATTT TGATGAACCA GAGAAGAATT CGTGGGTTTC ATCCTCTGTG 
TGCCAGCTGA GCCCCTCGGG CTTCTCAGGC GAAGAGCTGC CGTTTGTGGG ATTTTCGTAC 
AGCAAGGCAC TGGGGTATCT TGGTAGATCT GAGTCTGTCG TGTCGAGTCT GGACTCCCCT 
GCCAAGGTTA GCTCCATGGA AAAGAAACTT CTCATCAAAA GCAAAGAGCT CCAAGACTCC 
CAGGACAAGT GTCACAAGAT GGAGCAGGAA ATGACCCGGT TACATCGCAG AGTGTCAGAG 
GTGGAGGCTG TGCTTAGTCA GAAGGAGGTG GAGCTGAAGG CCTCTGAGAC TCAGAGATCC 
CTCCTGGAGC AGGACCTTGC TACCTACATC ACAGAATGCA GTAGCTTAAA GCGAAGTTTG 
GAGCAAGCGC GGATGGAGGT GTCCCAGGAG GATGACAAAG CTCTGCAGCT TCTCCACGAC 
ATCCGAGAGC AGAGCCGGAA GCTCCAGGAG ATCAAGGAGC AGGAGTACCA GGCTCAGGTG 
GAGGAGATGA GGCTGATGAT GAATCAGCTG GAAGAAGACC TGGTGTCAGC CCGCAGACGC 
AGCGATCTCT ACGAGTCTGA GCTGAGGGAG TCTCGGCTTG CCGCCGAGGA ATTCAAGCGG 
AAGGCAAACG AATGTCAGCA CAAACTGATG AAGGCTAAGG ACCAAGGGAA GCCTGAAGTG 
GGAGAATATT CCAAACTGGA GAAGATCAAT GCTGAGCAGC AGCTGAAGAT CCAGGAGCTC 
CAGGAGAAGC TGGAGAAGGC TGTAAAAGCC AGCACAGAGG CCACCGAGCT CCTGCAGAAT 
ATCCGCCAGG CAAAGGAGCG AGCAGAGCGG GAGCTAGAGA AGCTACACAA CCGGGAAGAC 
TCCTCCGAGG GCATCAAAAA GAAGCTGGTG GAAGCCGAGG AACGCCGCCA CTCCCTGGAG 
AACAAGGTAA AGAGACTAGA GACCATGGAG CGTAGAGAGA ACAGACTGAA GGATGACATC 
CAGACAAAGT CCGAACAGAT CCAGCAGATG GCTGATAAAA TTCTGGAGCT GGAGGAGAAA 
CATCGGGAGG CTCAGGTCTC AGCTCAACAT CTAGAAGTAC ACTTGAAACA GAAAGAACAG 
CACTACGAGG AAAAGATCAA GGTATTGGAC AATCAGATAA AGAAGGACCT GGCCGACAAG 
GAGAGCCTGG AGAACATGAT GCAGAGACAC GAGGAGGAGG CCCACGAGAA GGGCAAGATC 
CTCAGCGAGC AGAAGGCGAT GATCAACGCG ATGGATTCCA AGATCCGATC CCTGGAGCAG 
AGGATCGTGG AGCTGTCGGA AGCCAACAAG CTTGCGGCAA ACAGCAGTCT CTTCACCCAG 
AGGAACATGA AGGCCCAGGA AGAGATGATC TCAGAACTCA GGCAGCAGAA ATTTTACCTG 
GAGACGCAGG CCGGAAAGCT GGAGGCCCAG AACCGAAAGC TGGAAGAGCA ACTGGAGAAG 
ATCAGCCACC AAGATCACAG TGACAAGAGT CGGCTGCTGG AGCTGGAAAC AAGGCTGAGG 
GAGGTCAGCC TGGAGCACGA GGAGCAGAAG CTGGAGCTGA AGCGGCAGCT CACGGAGCTG 
CAGCTGTCCC TGCAGGAGCG CGAGTCCCAG CTGACGGCCC TGCAGGCCGC CCGGGCAGCG 
CTGGAGAGCC AGCTCCGCCA GGCGAAGACA GAGCTGGAGG AGACAACCGC GGAAGCGGAG 
GAGGAGATCC AGGCGCTCAC GGCACATCGA GATGAGATCC AGCGCAAATT CGATGCCCTT 
CGCAACAGCT GCACGGTCAT CACCGACCTG GAGGAGCAGC TGAACCAGCT CACCGAGGAC 
AACGCCGAGC TCAACAACCA AAACTTCTAC CTGTCCAAAC AACTCGATGA GGCTTCCGGG 
GCCAATGACG AGATTGTGCA GCTGCGGAGT GAGGTGGACC ATCTCCGCCG TGAGATCACG 
GAGCGGGAGA TGCAGCTCAC CAGCCAGAAG CAAACGATGG AGGCTCTGAA GACGACATGC 
ACCATGCTGG AGGAGCAGGT CCTGGACCTG GAGGCCCTGA ACGACGAGCT GCTGGAGAAG 
GAGCGCCAGT GGGAGGCCTG GCGGAGCGTC CTCGGCGACG AGAAGTCCCA GTTCGAGTGT 
CGAGTTCGAG AGCTACAGAG GATGCTGGAC ACCGAGAAGC AGAGCAGGGC TAGGGCCGAT 
CAGCGGATCA CCGAGTCGCG CCAGGTGGTG GAGTTGGCGG TGAAGGAACA CAAGGCCGAG 
ATTCTTGCTC TGCAGCAGGC TCTCAAGGAG CAGAAGCTCA AAGCCGAGAG CCTGTCGGAC 
AAGCTCAACG ACCTGGAGAA GAAGCACGCC ATGCTGGAGA TGAACGCCCG GAGCTTACAG 
CAGAAACTAG AGACAGAGCG GGAGCTCAAA CAGAGGCTTC TGGAGGAGCA AGCCAAATTG 
CAGCAGCAGA TGGACCTGCA GAAGAACCAC ATCTTCAGAC TGACGCAAGG GCTGCAGGAG 
GCGCTGGACC GGGCGGATCT GCTGAAGACA GAAAGGAGCG ACCTGGAGTA CCAGCTGGAA 
AACATTCAGG TTCTCTACTC TCACGAGAAA GTGAAAATGG AAGGCACAAT CTCTCAGCAA 
ACCAAACTCA TTGATTTCCT GCAAGCCAAA ATGGACCAGC CTGCTAAAAA GAAAAAGGGT 
TTATTTAGTC GACGGAAAGA GGACCCTGCT TTGCCCACAC AGGTTCCTCT GCAGTACAAT 
GAGCTGAAGC TAGCCCTGGA AAAGGAGAAA GCCCGATGCG CGGAGCTGGA GGAGGCCCTT 
CAGAAGACCC GCATCGAACT CCGCTCTGCG CGGGAGGAAG CTGCCCACCG CAAAGCCACG 
GACCACCCGC ACCCGTCTAC GCCAGCCACT GCGAGGCAGC AGATCGCCAT GTCTGCCATT 
GTGCGGTCGC CCGAGCACCA GCCCAGTGCC ATGAGCCTGC TTGCCCCACC ATCCAGCCGC 
AGAAAGGAGT CGTCAACTCC AGAGGAATTC AGCCGGCGTC TGAAAGAGCG CATGCACCAC 
AACATCCCTC ACCGGTTTAA TGTGGGCCTG AACATGAGAG CCACCAAGTG CGCCGTGTGT 
CTGGATACTG TGCACTTTGG ACGCCAGGCA TCCAAATGCC TCGAATGTCA AGTGATGTGT 
CACCCCAAAT GCTCCACCTG CTTGCCCGCT ACCTGTGGCC TGCCAGCTGA ATATGCCACA 
CACTTCACTG AGGCCTTCTG CCGGGACAAA ATGAACTCCC CGGGGCTCCA GAGCAAGGAG 
CCTGGCAGCA GCTTGCACCT GGAAGGGTGG ATGAAAGTGC CCAGAAATAA CAAACGGGGA 
CAGCAAGGCT GGGACAGGAA GTACATTGTT CTGGAGGGGT CAAAAGTCCT CATCTATGAC 
AATGAAGCCA GAGAAGCTGG ACAGAGGCCG GTGGAAGAAT TTGAGCTGTG CCTTCCTGAC 
GGGGACGTAT CTATTCATGG TGCCGTTGGT GCTTCAGAAC TTGCAAATAC CGCCAAAGCA 
GATGTCCCAT ACATCCTGAA GATGGAGTCT CACCCACACA CCACCTGCTG GCCTGGGAGA 
ACCCTCTACT TGCTAGCACC CAGCTTCCCC GACAAGCAGC GCTGGGTCAC CGCCTTAGAA 
TCTGTCGTCG CAGGTGGGAG AGTTTCTAGG GAAAAGGCCG AAGCCGATGC TAAATTACTT 
GGAAACTCTC TGCTGAAACT GGAGGGCGAT GACCGGCTTG ACATGAACTG CACCCTGCCC 
TTCAGTGACC AGGTAGTGCT GGTGGGCACG GAGGAAGGCC TCTACGCCCT GAATGTCTTG 
AAAAACTCCT TAACCCACAT CCCAGGGATT GGCGCAGTCT TCCAAATTTA CATCATCAAG 
GACCTGGAGA AGCTGCTCAT GATAGCAGGG GAAGAGCGGG CTCTGTGCCT GGTGGACGTG 
AAGAAGGTGA AGCAGTCCCT GGCGCAGTCA CACCTGCCTG CCCAGCCCGA CGTCTCCCCC 
AACATATTCG AAGCCGTCAA AGGCTGCCAC TTGTTCGCTG CTGGCAAGAT CGAGAACAGC 
CTGTGCATCT GCGCCGCTAT GCCAAGCAAA GTCGTCATCC TCCGCTACAA TGACAACCTC 
AGCAAGTACT GCATCCGCAA GGAGATCGAG ACCTCAGAGC CCTGCAGCTG TATCCACTTC 
ACCAACTACA GCATCCTCAT TGGGACCAAC AAATTCTATG AGATCGACAT GAAGCAGTAC 
ACGCTTGACG AGTTCCTGGA CAAGAACGAC CATTCCTTGG CACCTGCTGT GTTCGCCTCC 
TCGTCCAACA GCTTCCCTGT CTCCATTGTG CAGGCGAACA GCGCCGGGCA GCGAGAAGAA 
TACCTGCTGT GCTTCCACGA ATTTGGGGTG TTCGTGGATT CTTACGGAAG ACGTAGCCGC 
ACAGATGATC TCAAGTGGAG TCGCTTACCT CTGGCCTTCG CCTACAGAGA ACCTTATCTG 
TTTGTGACTC ACTTCAACTC CCTGGAAGTC ATTGAGATCC AGGCCAGATC CTCACTGGGG 
AGCCCTGCCC GAGCATATCT GGAAATTCCA AACCCTCGCT ACCTGGGCCC CGCGATTTCC 
TCCGGAGCGA TTTACCTGGC CTCCTCATAC CAGGACAAGT TAAGGGTCAT ATGCTGCAAA 
GGAAACCTCG TGAAGGAGTC AGGCACTGAG CAGCACCGGG TGCCCTCCAC CTCCCGCAGC 
AGCCCCAACA AGCGAGGCCC ACCAACATAC AACGAGCACA TCACCAAACG CGTGGCCTCC 
AGCCCGGCGC CACCGGAAGG CCCCAGCCAC CCCCGAGAGC CAAGCACACC GCACCGCTAC 
CGAGACAGAG AGGGTCGGAC AGAGCTGCGC AGGGACAAGT CTCCAGGCCG CCCTCTGGAG 
CGGGAGAAGT CCCCAGGCCG AATGCTCAGC ACTAGGAGAG AGCGGTCCCC AGGGAGACTG 
TTTGAAGACA GCAGCAGGGG CCGGCTGCCT GCAGGAGCAG TGAGGACCCC ACTGTCCCAG 
GTTAACAAGG TCTGGGACCA GTCTTCAGTA TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cit ( XM_006530143.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006530206.1 Mus musculus citron (Cit), transcript variant X17, mRNA.

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CloneID	OMu35783
Clone ID Related Accession (Same CDS sequence)	XM_006530143.3 , XM_006530143.4
Accession Version	XM_006530143.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6087bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	citron Rho-interacting kinase isoform X13
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000071.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006530143.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTGAAGT TCAAGTATGG TGTGCGGAAC CCGCCGGAGG CCAGTGCCTC CGAGCCCATT 
GCCAGTCGGG CCTCCAGGCT AAATCTCTTC TTCCAGGGGA AACCGCCCCT CATGACTCAA 
CAGCAGATGT CTGCTCTTTC CCGGGAAGGG ATGCTAGACG CCCTCTTCGC TCTCTTTGAA 
GAGTGCAGCC AACCCGCCCT GATGAAGATG AAGCACGTGA GCAGCTTTGT CCAGAAGTAT 
TCCGACACCA TAGCCGAGTT GCGGGAGCTG CAGCCGTCGG CGAGAGACTT CGAAGTTCGA 
AGCCTTGTGG GCTGTGGTCA CTTCGCTGAA GTGCAGGTGG TTAGAGAGAA GGCGACCGGG 
GACGTCTATG CCATGAAAAT CATGAAGAAG AAGGCTTTGC TGGCCCAGGA ACAGGTTTCA 
TTTTTCGAGG AGGAGAGGAA CATATTATCT CGGAGCACGA GTCCTTGGAT CCCCCAGTTA 
CAGTACGCCT TTCAGGACAA AAATAACCTT TACCTGGTCA TGGAATATCA GCCTGGAGGG 
GATTTGCTGT CGCTTCTGAA CAGATACGAG GACCAATTAG ATGAGAGCAT GATCCAGTTT 
TACCTTGCTG AGCTGATTTT GGCTGTCCAC AGCGTGCACC AGATGGGATA TGTGCATCGA 
GACATCAAGC CCGAGAACAT CCTCATCGAC CGGACGGGAC ACATCAAGCT GGTGGATTTT 
GGATCAGCCG CTAAGATGAA TTCAAATAAG GTGGATGCCA AACTCCCCAT TGGGACCCCG 
GATTACATGG CTCCGGAAGT GTTGACCGTG ATGAACGAGG ACCGAAGGGG CACATACGGC 
TTGGACTGTG ACTGGTGGTC TGTCGGAGTT GTTGCTTATG AGATGGTTTA TGGGAAGACC 
CCATTCACAG AGGGAACCTC CGCCCGGACC TTCAACAACA TCATGAACTT CCAGCGGTTT 
TTGAAGTTCC CAGATGACCC CAAAGTTAGC AGTGAGCTCC TTGATCTGCT TCAGAGTCTG 
CTGTGTGTCC AGAAAGAGAG ACTGAAGTTC GAGGGTCTCT GCTGCCACCC TTTCTTTGCC 
AGAACGGACT GGAACAACAT CCGTAACTCT CCTCCCCCCT TCGTCCCCAC CCTCAAGTCT 
GACGATGACA CCTCCAATTT TGATGAACCA GAGAAGAATT CGTGGGTTTC ATCCTCTGTG 
TGCCAGCTGA GCCCCTCGGG CTTCTCAGGC GAAGAGCTGC CGTTTGTGGG ATTTTCGTAC 
AGCAAGGCAC TGGGGTATCT TGGTAGATCT GAGTCTGTCG TGTCGAGTCT GGACTCCCCT 
GCCAAGGTTA GCTCCATGGA AAAGAAACTT CTCATCAAAA GCAAAGAGCT CCAAGACTCC 
CAGGACAAGT GTCACAAGAT GGAGCAGGAA ATGACCCGGT TACATCGCAG AGTGTCAGAG 
GTGGAGGCTG TGCTTAGTCA GAAGGAGGTG GAGCTGAAGG CCTCTGAGAC TCAGAGATCC 
CTCCTGGAGC AGGACCTTGC TACCTACATC ACAGAATGCA GTAGCTTAAA GCGAAGTTTG 
GAGCAAGCGC GGATGGAGGT GTCCCAGGAG GATGACAAAG CTCTGCAGCT TCTCCACGAC 
ATCCGAGAGC AGAGCCGGAA GCTCCAGGAG ATCAAGGAGC AGGAGTACCA GGCTCAGGTG 
GAGGAGATGA GGCTGATGAT GAATCAGCTG GAAGAAGACC TGGTGTCAGC CCGCAGACGC 
AGCGATCTCT ACGAGTCTGA GCTGAGGGAG TCTCGGCTTG CCGCCGAGGA ATTCAAGCGG 
AAGGCAAACG AATGTCAGCA CAAACTGATG AAGGCTAAGG ACCAAGGGAA GCCTGAAGTG 
GGAGAATATT CCAAACTGGA GAAGATCAAT GCTGAGCAGC AGCTGAAGAT CCAGGAGCTC 
CAGGAGAAGC TGGAGAAGGC TGTAAAAGCC AGCACAGAGG CCACCGAGCT CCTGCAGAAT 
ATCCGCCAGG CAAAGGAGCG AGCAGAGCGG GAGCTAGAGA AGCTACACAA CCGGGAAGAC 
TCCTCCGAGG GCATCAAAAA GAAGCTGGTG GAAGCCGAGG AGCTGGAGGA GAAACATCGG 
GAGGCTCAGG TCTCAGCTCA ACATCTAGAA GTACACTTGA AACAGAAAGA ACAGCACTAC 
GAGGAAAAGA TCAAGGTATT GGACAATCAG ATAAAGAAGG ACCTGGCCGA CAAGGAGAGC 
CTGGAGAACA TGATGCAGAG ACACGAGGAG GAGGCCCACG AGAAGGGCAA GATCCTCAGC 
GAGCAGAAGG CGATGATCAA CGCGATGGAT TCCAAGATCC GATCCCTGGA GCAGAGGATC 
GTGGAGCTGT CGGAAGCCAA CAAGCTTGCG GCAAACAGCA GTCTCTTCAC CCAGAGGAAC 
ATGAAGGCCC AGGAAGAGAT GATCTCAGAA CTCAGGCAGC AGAAATTTTA CCTGGAGACG 
CAGGCCGGAA AGCTGGAGGC CCAGAACCGA AAGCTGGAAG AGCAACTGGA GAAGATCAGC 
CACCAAGATC ACAGTGACAA GAGTCGGCTG CTGGAGCTGG AAACAAGGCT GAGGGAGGTC 
AGCCTGGAGC ACGAGGAGCA GAAGCTGGAG CTGAAGCGGC AGCTCACGGA GCTGCAGCTG 
TCCCTGCAGG AGCGCGAGTC CCAGCTGACG GCCCTGCAGG CCGCCCGGGC AGCGCTGGAG 
AGCCAGCTCC GCCAGGCGAA GACAGAGCTG GAGGAGACAA CCGCGGAAGC GGAGGAGGAG 
ATCCAGGCGC TCACGGCACA TCGAGATGAG ATCCAGCGCA AATTCGATGC CCTTCGCAAC 
AGCTGCACGG TCATCACCGA CCTGGAGGAG CAGCTGAACC AGCTCACCGA GGACAACGCC 
GAGCTCAACA ACCAAAACTT CTACCTGTCC AAACAACTCG ATGAGGCTTC CGGGGCCAAT 
GACGAGATTG TGCAGCTGCG GAGTGAGGTG GACCATCTCC GCCGTGAGAT CACGGAGCGG 
GAGATGCAGC TCACCAGCCA GAAGCAAACG ATGGAGGCTC TGAAGACGAC ATGCACCATG 
CTGGAGGAGC AGGTCCTGGA CCTGGAGGCC CTGAACGACG AGCTGCTGGA GAAGGAGCGC 
CAGTGGGAGG CCTGGCGGAG CGTCCTCGGC GACGAGAAGT CCCAGTTCGA GTGTCGAGTT 
CGAGAGCTAC AGAGGATGCT GGACACCGAG AAGCAGAGCA GGGCTAGGGC CGATCAGCGG 
ATCACCGAGT CGCGCCAGGT GGTGGAGTTG GCGGTGAAGG AACACAAGGC CGAGATTCTT 
GCTCTGCAGC AGGCTCTCAA GGAGCAGAAG CTCAAAGCCG AGAGCCTGTC GGACAAGCTC 
AACGACCTGG AGAAGAAGCA CGCCATGCTG GAGATGAACG CCCGGAGCTT ACAGCAGAAA 
CTAGAGACAG AGCGGGAGCT CAAACAGAGG CTTCTGGAGG AGCAAGCCAA ATTGCAGCAG 
CAGATGGACC TGCAGAAGAA CCACATCTTC AGACTGACGC AAGGGCTGCA GGAGGCGCTG 
GACCGGGCGG ATCTGCTGAA GACAGAAAGG AGCGACCTGG AGTACCAGCT GGAAAACATT 
CAGGTTCTCT ACTCTCACGA GAAAGTGAAA ATGGAAGGCA CAATCTCTCA GCAAACCAAA 
CTCATTGATT TCCTGCAAGC CAAAATGGAC CAGCCTGCTA AAAAGAAAAA GGGTTTATTT 
AGTCGACGGA AAGAGGACCC TGCTTTGCCC ACACAGGTTC CTCTGCAGTA CAATGAGCTG 
AAGCTAGCCC TGGAAAAGGA GAAAGCCCGA TGCGCGGAGC TGGAGGAGGC CCTTCAGAAG 
ACCCGCATCG AACTCCGCTC TGCGCGGGAG GAAGCTGCCC ACCGCAAAGC CACGGACCAC 
CCGCACCCGT CTACGCCAGC CACTGCGAGG CAGCAGATCG CCATGTCTGC CATTGTGCGG 
TCGCCCGAGC ACCAGCCCAG TGCCATGAGC CTGCTTGCCC CACCATCCAG CCGCAGAAAG 
GAGTCGTCAA CTCCAGAGGA ATTCAGCCGG CGTCTGAAAG AGCGCATGCA CCACAACATC 
CCTCACCGGT TTAATGTGGG CCTGAACATG AGAGCCACCA AGTGCGCCGT GTGTCTGGAT 
ACTGTGCACT TTGGACGCCA GGCATCCAAA TGCCTCGAAT GTCAAGTGAT GTGTCACCCC 
AAATGCTCCA CCTGCTTGCC CGCTACCTGT GGCCTGCCAG CTGAATATGC CACACACTTC 
ACTGAGGCCT TCTGCCGGGA CAAAATGAAC TCCCCGGGGC TCCAGAGCAA GGAGCCTGGC 
AGCAGCTTGC ACCTGGAAGG GTGGATGAAA GTGCCCAGAA ATAACAAACG GGGACAGCAA 
GGCTGGGACA GGAAGTACAT TGTTCTGGAG GGGTCAAAAG TCCTCATCTA TGACAATGAA 
GCCAGAGAAG CTGGACAGAG GCCGGTGGAA GAATTTGAGC TGTGCCTTCC TGACGGGGAC 
GTATCTATTC ATGGTGCCGT TGGTGCTTCA GAACTTGCAA ATACCGCCAA AGCAGATGTC 
CCATACATCC TGAAGATGGA GTCTCACCCA CACACCACCT GCTGGCCTGG GAGAACCCTC 
TACTTGCTAG CACCCAGCTT CCCCGACAAG CAGCGCTGGG TCACCGCCTT AGAATCTGTC 
GTCGCAGGTG GGAGAGTTTC TAGGGAAAAG GCCGAAGCCG ATGCTAAATT ACTTGGAAAC 
TCTCTGCTGA AACTGGAGGG CGATGACCGG CTTGACATGA ACTGCACCCT GCCCTTCAGT 
GACCAGGTAG TGCTGGTGGG CACGGAGGAA GGCCTCTACG CCCTGAATGT CTTGAAAAAC 
TCCTTAACCC ACATCCCAGG GATTGGCGCA GTCTTCCAAA TTTACATCAT CAAGGACCTG 
GAGAAGCTGC TCATGATAGC AGGGGAAGAG CGGGCTCTGT GCCTGGTGGA CGTGAAGAAG 
GTGAAGCAGT CCCTGGCGCA GTCACACCTG CCTGCCCAGC CCGACGTCTC CCCCAACATA 
TTCGAAGCCG TCAAAGGCTG CCACTTGTTC GCTGCTGGCA AGATCGAGAA CAGCCTGTGC 
ATCTGCGCCG CTATGCCAAG CAAAGTCGTC ATCCTCCGCT ACAATGACAA CCTCAGCAAG 
TACTGCATCC GCAAGGAGAT CGAGACCTCA GAGCCCTGCA GCTGTATCCA CTTCACCAAC 
TACAGCATCC TCATTGGGAC CAACAAATTC TATGAGATCG ACATGAAGCA GTACACGCTT 
GACGAGTTCC TGGACAAGAA CGACCATTCC TTGGCACCTG CTGTGTTCGC CTCCTCGTCC 
AACAGCTTCC CTGTCTCCAT TGTGCAGGCG AACAGCGCCG GGCAGCGAGA AGAATACCTG 
CTGTGCTTCC ACGAATTTGG GGTGTTCGTG GATTCTTACG GAAGACGTAG CCGCACAGAT 
GATCTCAAGT GGAGTCGCTT ACCTCTGGCC TTCGCCTACA GAGAACCTTA TCTGTTTGTG 
ACTCACTTCA ACTCCCTGGA AGTCATTGAG ATCCAGGCCA GATCCTCACT GGGGAGCCCT 
GCCCGAGCAT ATCTGGAAAT TCCAAACCCT CGCTACCTGG GCCCCGCGAT TTCCTCCGGA 
GCGATTTACC TGGCCTCCTC ATACCAGGAC AAGTTAAGGG TCATATGCTG CAAAGGAAAC 
CTCGTGAAGG AGTCAGGCAC TGAGCAGCAC CGGGTGCCCT CCACCTCCCG CAGCAGCCCC 
AACAAGCGAG GCCCACCAAC ATACAACGAG CACATCACCA AACGCGTGGC CTCCAGCCCG 
GCGCCACCGG AAGGCCCCAG CCACCCCCGA GAGCCAAGCA CACCGCACCG CTACCGAGAC 
AGAGAGGGTC GGACAGAGCT GCGCAGGGAC AAGTCTCCAG GCCGCCCTCT GGAGCGGGAG 
AAGTCCCCAG GCCGAATGCT CAGCACTAGG AGAGAGCGGT CCCCAGGGAG ACTGTTTGAA 
GACAGCAGCA GGGGCCGGCT GCCTGCAGGA GCAGTGAGGA CCCCACTGTC CCAGGTTAAC 
AAGGTCTGGG ACCAGTCTTC AGTATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006530206.1
CDS	157..6243
Translation	MLKFKYGVRNPPEASASEPIASRASRLNLFFQGKPPLMTQQQMSALSREGMLDALFALFEECSQPALMKMKHVSSFVQKYSDTIAELRELQPSARDFEVRSLVGCGHFAEVQVVREKATGDVYAMKIMKKKALLAQEQVSFFEEERNILSRSTSPWIPQLQYAFQDKNNLYLVMEYQPGGDLLSLLNRYEDQLDESMIQFYLAELILAVHSVHQMGYVHRDIKPENILIDRTGHIKLVDFGSAAKMNSNKVDAKLPIGTPDYMAPEVLTVMNEDRRGTYGLDCDWWSVGVVAYEMVYGKTPFTEGTSARTFNNIMNFQRFLKFPDDPKVSSELLDLLQSLLCVQKERLKFEGLCCHPFFARTDWNNIRNSPPPFVPTLKSDDDTSNFDEPEKNSWVSSSVCQLSPSGFSGEELPFVGFSYSKALGYLGRSESVVSSLDSPAKVSSMEKKLLIKSKELQDSQDKCHKMEQEMTRLHRRVSEVEAVLSQKEVELKASETQRSLLEQDLATYITECSSLKRSLEQARMEVSQEDDKALQLLHDIREQSRKLQEIKEQEYQAQVEEMRLMMNQLEEDLVSARRRSDLYESELRESRLAAEEFKRKANECQHKLMKAKDQGKPEVGEYSKLEKINAEQQLKIQELQEKLEKAVKASTEATELLQNIRQAKERAERELEKLHNREDSSEGIKKKLVEAEELEEKHREAQVSAQHLEVHLKQKEQHYEEKIKVLDNQIKKDLADKESLENMMQRHEEEAHEKGKILSEQKAMINAMDSKIRSLEQRIVELSEANKLAANSSLFTQRNMKAQEEMISELRQQKFYLETQAGKLEAQNRKLEEQLEKISHQDHSDKSRLLELETRLREVSLEHEEQKLELKRQLTELQLSLQERESQLTALQAARAALESQLRQAKTELEETTAEAEEEIQALTAHRDEIQRKFDALRNSCTVITDLEEQLNQLTEDNAELNNQNFYLSKQLDEASGANDEIVQLRSEVDHLRREITEREMQLTSQKQTMEALKTTCTMLEEQVLDLEALNDELLEKERQWEAWRSVLGDEKSQFECRVRELQRMLDTEKQSRARADQRITESRQVVELAVKEHKAEILALQQALKEQKLKAESLSDKLNDLEKKHAMLEMNARSLQQKLETERELKQRLLEEQAKLQQQMDLQKNHIFRLTQGLQEALDRADLLKTERSDLEYQLENIQVLYSHEKVKMEGTISQQTKLIDFLQAKMDQPAKKKKGLFSRRKEDPALPTQVPLQYNELKLALEKEKARCAELEEALQKTRIELRSAREEAAHRKATDHPHPSTPATARQQIAMSAIVRSPEHQPSAMSLLAPPSSRRKESSTPEEFSRRLKERMHHNIPHRFNVGLNMRATKCAVCLDTVHFGRQASKCLECQVMCHPKCSTCLPATCGLPAEYATHFTEAFCRDKMNSPGLQSKEPGSSLHLEGWMKVPRNNKRGQQGWDRKYIVLEGSKVLIYDNEAREAGQRPVEEFELCLPDGDVSIHGAVGASELANTAKADVPYILKMESHPHTTCWPGRTLYLLAPSFPDKQRWVTALESVVAGGRVSREKAEADAKLLGNSLLKLEGDDRLDMNCTLPFSDQVVLVGTEEGLYALNVLKNSLTHIPGIGAVFQIYIIKDLEKLLMIAGEERALCLVDVKKVKQSLAQSHLPAQPDVSPNIFEAVKGCHLFAAGKIENSLCICAAMPSKVVILRYNDNLSKYCIRKEIETSEPCSCIHFTNYSILIGTNKFYEIDMKQYTLDEFLDKNDHSLAPAVFASSSNSFPVSIVQANSAGQREEYLLCFHEFGVFVDSYGRRSRTDDLKWSRLPLAFAYREPYLFVTHFNSLEVIEIQARSSLGSPARAYLEIPNPRYLGPAISSGAIYLASSYQDKLRVICCKGNLVKESGTEQHRVPSTSRSSPNKRGPPTYNEHITKRVASSPAPPEGPSHPREPSTPHRYRDREGRTELRRDKSPGRPLEREKSPGRMLSTRRERSPGRLFEDSSRGRLPAGAVRTPLSQVNKVWDQSSV

Target ORF information:

RefSeq Version	XM_006530143.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus citron (Cit), transcript variant X17, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006530143.4

ORF Insert Sequence:

ATGTTGAAGT TCAAGTATGG TGTGCGGAAC CCGCCGGAGG CCAGTGCCTC CGAGCCCATT 
GCCAGTCGGG CCTCCAGGCT AAATCTCTTC TTCCAGGGGA AACCGCCCCT CATGACTCAA 
CAGCAGATGT CTGCTCTTTC CCGGGAAGGG ATGCTAGACG CCCTCTTCGC TCTCTTTGAA 
GAGTGCAGCC AACCCGCCCT GATGAAGATG AAGCACGTGA GCAGCTTTGT CCAGAAGTAT 
TCCGACACCA TAGCCGAGTT GCGGGAGCTG CAGCCGTCGG CGAGAGACTT CGAAGTTCGA 
AGCCTTGTGG GCTGTGGTCA CTTCGCTGAA GTGCAGGTGG TTAGAGAGAA GGCGACCGGG 
GACGTCTATG CCATGAAAAT CATGAAGAAG AAGGCTTTGC TGGCCCAGGA ACAGGTTTCA 
TTTTTCGAGG AGGAGAGGAA CATATTATCT CGGAGCACGA GTCCTTGGAT CCCCCAGTTA 
CAGTACGCCT TTCAGGACAA AAATAACCTT TACCTGGTCA TGGAATATCA GCCTGGAGGG 
GATTTGCTGT CGCTTCTGAA CAGATACGAG GACCAATTAG ATGAGAGCAT GATCCAGTTT 
TACCTTGCTG AGCTGATTTT GGCTGTCCAC AGCGTGCACC AGATGGGATA TGTGCATCGA 
GACATCAAGC CCGAGAACAT CCTCATCGAC CGGACGGGAC ACATCAAGCT GGTGGATTTT 
GGATCAGCCG CTAAGATGAA TTCAAATAAG GTGGATGCCA AACTCCCCAT TGGGACCCCG 
GATTACATGG CTCCGGAAGT GTTGACCGTG ATGAACGAGG ACCGAAGGGG CACATACGGC 
TTGGACTGTG ACTGGTGGTC TGTCGGAGTT GTTGCTTATG AGATGGTTTA TGGGAAGACC 
CCATTCACAG AGGGAACCTC CGCCCGGACC TTCAACAACA TCATGAACTT CCAGCGGTTT 
TTGAAGTTCC CAGATGACCC CAAAGTTAGC AGTGAGCTCC TTGATCTGCT TCAGAGTCTG 
CTGTGTGTCC AGAAAGAGAG ACTGAAGTTC GAGGGTCTCT GCTGCCACCC TTTCTTTGCC 
AGAACGGACT GGAACAACAT CCGTAACTCT CCTCCCCCCT TCGTCCCCAC CCTCAAGTCT 
GACGATGACA CCTCCAATTT TGATGAACCA GAGAAGAATT CGTGGGTTTC ATCCTCTGTG 
TGCCAGCTGA GCCCCTCGGG CTTCTCAGGC GAAGAGCTGC CGTTTGTGGG ATTTTCGTAC 
AGCAAGGCAC TGGGGTATCT TGGTAGATCT GAGTCTGTCG TGTCGAGTCT GGACTCCCCT 
GCCAAGGTTA GCTCCATGGA AAAGAAACTT CTCATCAAAA GCAAAGAGCT CCAAGACTCC 
CAGGACAAGT GTCACAAGAT GGAGCAGGAA ATGACCCGGT TACATCGCAG AGTGTCAGAG 
GTGGAGGCTG TGCTTAGTCA GAAGGAGGTG GAGCTGAAGG CCTCTGAGAC TCAGAGATCC 
CTCCTGGAGC AGGACCTTGC TACCTACATC ACAGAATGCA GTAGCTTAAA GCGAAGTTTG 
GAGCAAGCGC GGATGGAGGT GTCCCAGGAG GATGACAAAG CTCTGCAGCT TCTCCACGAC 
ATCCGAGAGC AGAGCCGGAA GCTCCAGGAG ATCAAGGAGC AGGAGTACCA GGCTCAGGTG 
GAGGAGATGA GGCTGATGAT GAATCAGCTG GAAGAAGACC TGGTGTCAGC CCGCAGACGC 
AGCGATCTCT ACGAGTCTGA GCTGAGGGAG TCTCGGCTTG CCGCCGAGGA ATTCAAGCGG 
AAGGCAAACG AATGTCAGCA CAAACTGATG AAGGCTAAGG ACCAAGGGAA GCCTGAAGTG 
GGAGAATATT CCAAACTGGA GAAGATCAAT GCTGAGCAGC AGCTGAAGAT CCAGGAGCTC 
CAGGAGAAGC TGGAGAAGGC TGTAAAAGCC AGCACAGAGG CCACCGAGCT CCTGCAGAAT 
ATCCGCCAGG CAAAGGAGCG AGCAGAGCGG GAGCTAGAGA AGCTACACAA CCGGGAAGAC 
TCCTCCGAGG GCATCAAAAA GAAGCTGGTG GAAGCCGAGG AGCTGGAGGA GAAACATCGG 
GAGGCTCAGG TCTCAGCTCA ACATCTAGAA GTACACTTGA AACAGAAAGA ACAGCACTAC 
GAGGAAAAGA TCAAGGTATT GGACAATCAG ATAAAGAAGG ACCTGGCCGA CAAGGAGAGC 
CTGGAGAACA TGATGCAGAG ACACGAGGAG GAGGCCCACG AGAAGGGCAA GATCCTCAGC 
GAGCAGAAGG CGATGATCAA CGCGATGGAT TCCAAGATCC GATCCCTGGA GCAGAGGATC 
GTGGAGCTGT CGGAAGCCAA CAAGCTTGCG GCAAACAGCA GTCTCTTCAC CCAGAGGAAC 
ATGAAGGCCC AGGAAGAGAT GATCTCAGAA CTCAGGCAGC AGAAATTTTA CCTGGAGACG 
CAGGCCGGAA AGCTGGAGGC CCAGAACCGA AAGCTGGAAG AGCAACTGGA GAAGATCAGC 
CACCAAGATC ACAGTGACAA GAGTCGGCTG CTGGAGCTGG AAACAAGGCT GAGGGAGGTC 
AGCCTGGAGC ACGAGGAGCA GAAGCTGGAG CTGAAGCGGC AGCTCACGGA GCTGCAGCTG 
TCCCTGCAGG AGCGCGAGTC CCAGCTGACG GCCCTGCAGG CCGCCCGGGC AGCGCTGGAG 
AGCCAGCTCC GCCAGGCGAA GACAGAGCTG GAGGAGACAA CCGCGGAAGC GGAGGAGGAG 
ATCCAGGCGC TCACGGCACA TCGAGATGAG ATCCAGCGCA AATTCGATGC CCTTCGCAAC 
AGCTGCACGG TCATCACCGA CCTGGAGGAG CAGCTGAACC AGCTCACCGA GGACAACGCC 
GAGCTCAACA ACCAAAACTT CTACCTGTCC AAACAACTCG ATGAGGCTTC CGGGGCCAAT 
GACGAGATTG TGCAGCTGCG GAGTGAGGTG GACCATCTCC GCCGTGAGAT CACGGAGCGG 
GAGATGCAGC TCACCAGCCA GAAGCAAACG ATGGAGGCTC TGAAGACGAC ATGCACCATG 
CTGGAGGAGC AGGTCCTGGA CCTGGAGGCC CTGAACGACG AGCTGCTGGA GAAGGAGCGC 
CAGTGGGAGG CCTGGCGGAG CGTCCTCGGC GACGAGAAGT CCCAGTTCGA GTGTCGAGTT 
CGAGAGCTAC AGAGGATGCT GGACACCGAG AAGCAGAGCA GGGCTAGGGC CGATCAGCGG 
ATCACCGAGT CGCGCCAGGT GGTGGAGTTG GCGGTGAAGG AACACAAGGC CGAGATTCTT 
GCTCTGCAGC AGGCTCTCAA GGAGCAGAAG CTCAAAGCCG AGAGCCTGTC GGACAAGCTC 
AACGACCTGG AGAAGAAGCA CGCCATGCTG GAGATGAACG CCCGGAGCTT ACAGCAGAAA 
CTAGAGACAG AGCGGGAGCT CAAACAGAGG CTTCTGGAGG AGCAAGCCAA ATTGCAGCAG 
CAGATGGACC TGCAGAAGAA CCACATCTTC AGACTGACGC AAGGGCTGCA GGAGGCGCTG 
GACCGGGCGG ATCTGCTGAA GACAGAAAGG AGCGACCTGG AGTACCAGCT GGAAAACATT 
CAGGTTCTCT ACTCTCACGA GAAAGTGAAA ATGGAAGGCA CAATCTCTCA GCAAACCAAA 
CTCATTGATT TCCTGCAAGC CAAAATGGAC CAGCCTGCTA AAAAGAAAAA GGGTTTATTT 
AGTCGACGGA AAGAGGACCC TGCTTTGCCC ACACAGGTTC CTCTGCAGTA CAATGAGCTG 
AAGCTAGCCC TGGAAAAGGA GAAAGCCCGA TGCGCGGAGC TGGAGGAGGC CCTTCAGAAG 
ACCCGCATCG AACTCCGCTC TGCGCGGGAG GAAGCTGCCC ACCGCAAAGC CACGGACCAC 
CCGCACCCGT CTACGCCAGC CACTGCGAGG CAGCAGATCG CCATGTCTGC CATTGTGCGG 
TCGCCCGAGC ACCAGCCCAG TGCCATGAGC CTGCTTGCCC CACCATCCAG CCGCAGAAAG 
GAGTCGTCAA CTCCAGAGGA ATTCAGCCGG CGTCTGAAAG AGCGCATGCA CCACAACATC 
CCTCACCGGT TTAATGTGGG CCTGAACATG AGAGCCACCA AGTGCGCCGT GTGTCTGGAT 
ACTGTGCACT TTGGACGCCA GGCATCCAAA TGCCTCGAAT GTCAAGTGAT GTGTCACCCC 
AAATGCTCCA CCTGCTTGCC CGCTACCTGT GGCCTGCCAG CTGAATATGC CACACACTTC 
ACTGAGGCCT TCTGCCGGGA CAAAATGAAC TCCCCGGGGC TCCAGAGCAA GGAGCCTGGC 
AGCAGCTTGC ACCTGGAAGG GTGGATGAAA GTGCCCAGAA ATAACAAACG GGGACAGCAA 
GGCTGGGACA GGAAGTACAT TGTTCTGGAG GGGTCAAAAG TCCTCATCTA TGACAATGAA 
GCCAGAGAAG CTGGACAGAG GCCGGTGGAA GAATTTGAGC TGTGCCTTCC TGACGGGGAC 
GTATCTATTC ATGGTGCCGT TGGTGCTTCA GAACTTGCAA ATACCGCCAA AGCAGATGTC 
CCATACATCC TGAAGATGGA GTCTCACCCA CACACCACCT GCTGGCCTGG GAGAACCCTC 
TACTTGCTAG CACCCAGCTT CCCCGACAAG CAGCGCTGGG TCACCGCCTT AGAATCTGTC 
GTCGCAGGTG GGAGAGTTTC TAGGGAAAAG GCCGAAGCCG ATGCTAAATT ACTTGGAAAC 
TCTCTGCTGA AACTGGAGGG CGATGACCGG CTTGACATGA ACTGCACCCT GCCCTTCAGT 
GACCAGGTAG TGCTGGTGGG CACGGAGGAA GGCCTCTACG CCCTGAATGT CTTGAAAAAC 
TCCTTAACCC ACATCCCAGG GATTGGCGCA GTCTTCCAAA TTTACATCAT CAAGGACCTG 
GAGAAGCTGC TCATGATAGC AGGGGAAGAG CGGGCTCTGT GCCTGGTGGA CGTGAAGAAG 
GTGAAGCAGT CCCTGGCGCA GTCACACCTG CCTGCCCAGC CCGACGTCTC CCCCAACATA 
TTCGAAGCCG TCAAAGGCTG CCACTTGTTC GCTGCTGGCA AGATCGAGAA CAGCCTGTGC 
ATCTGCGCCG CTATGCCAAG CAAAGTCGTC ATCCTCCGCT ACAATGACAA CCTCAGCAAG 
TACTGCATCC GCAAGGAGAT CGAGACCTCA GAGCCCTGCA GCTGTATCCA CTTCACCAAC 
TACAGCATCC TCATTGGGAC CAACAAATTC TATGAGATCG ACATGAAGCA GTACACGCTT 
GACGAGTTCC TGGACAAGAA CGACCATTCC TTGGCACCTG CTGTGTTCGC CTCCTCGTCC 
AACAGCTTCC CTGTCTCCAT TGTGCAGGCG AACAGCGCCG GGCAGCGAGA AGAATACCTG 
CTGTGCTTCC ACGAATTTGG GGTGTTCGTG GATTCTTACG GAAGACGTAG CCGCACAGAT 
GATCTCAAGT GGAGTCGCTT ACCTCTGGCC TTCGCCTACA GAGAACCTTA TCTGTTTGTG 
ACTCACTTCA ACTCCCTGGA AGTCATTGAG ATCCAGGCCA GATCCTCACT GGGGAGCCCT 
GCCCGAGCAT ATCTGGAAAT TCCAAACCCT CGCTACCTGG GCCCCGCGAT TTCCTCCGGA 
GCGATTTACC TGGCCTCCTC ATACCAGGAC AAGTTAAGGG TCATATGCTG CAAAGGAAAC 
CTCGTGAAGG AGTCAGGCAC TGAGCAGCAC CGGGTGCCCT CCACCTCCCG CAGCAGCCCC 
AACAAGCGAG GCCCACCAAC ATACAACGAG CACATCACCA AACGCGTGGC CTCCAGCCCG 
GCGCCACCGG AAGGCCCCAG CCACCCCCGA GAGCCAAGCA CACCGCACCG CTACCGAGAC 
AGAGAGGGTC GGACAGAGCT GCGCAGGGAC AAGTCTCCAG GCCGCCCTCT GGAGCGGGAG 
AAGTCCCCAG GCCGAATGCT CAGCACTAGG AGAGAGCGGT CCCCAGGGAG ACTGTTTGAA 
GACAGCAGCA GGGGCCGGCT GCCTGCAGGA GCAGTGAGGA CCCCACTGTC CCAGGTTAAC 
AAGGTCTGGG ACCAGTCTTC AGTATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cit ( XM_006530145.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006530208.1 Mus musculus citron (Cit), transcript variant X19, mRNA.

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CloneID	OMu35785
Clone ID Related Accession (Same CDS sequence)	XM_006530145.3 , XM_006530145.4
Accession Version	XM_006530145.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6042bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	citron Rho-interacting kinase isoform X15
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000071.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006530145.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTGAAGT TCAAGTATGG TGTGCGGAAC CCGCCGGAGG CCAGTGCCTC CGAGCCCATT 
GCCAGTCGGG CCTCCAGGCT AAATCTCTTC TTCCAGGGGA AACCGCCCCT CATGACTCAA 
CAGCAGATGT CTGCTCTTTC CCGGGAAGGG ATGCTAGACG CCCTCTTCGC TCTCTTTGAA 
GAGTGCAGCC AACCCGCCCT GATGAAGATG AAGCACGTGA GCAGCTTTGT CCAGAAGTAT 
TCCGACACCA TAGCCGAGTT GCGGGAGCTG CAGCCGTCGG CGAGAGACTT CGAAGTTCGA 
AGCCTTGTGG GCTGTGGTCA CTTCGCTGAA GTGCAGGTGG TTAGAGAGAA GGCGACCGGG 
GACGTCTATG CCATGAAAAT CATGAAGAAG AAGGCTTTGC TGGCCCAGGA ACAGGTTTCA 
TTTTTCGAGG AGGAGAGGAA CATATTATCT CGGAGCACGA GTCCTTGGAT CCCCCAGTTA 
CAGTACGCCT TTCAGGACAA AAATAACCTT TACCTGGTCA TGGAATATCA GCCTGGAGGG 
GATTTGCTGT CGCTTCTGAA CAGATACGAG GACCAATTAG ATGAGAGCAT GATCCAGTTT 
TACCTTGCTG AGCTGATTTT GGCTGTCCAC AGCGTGCACC AGATGGGATA TGTGCATCGA 
GACATCAAGC CCGAGAACAT CCTCATCGAC CGGACGGGAC ACATCAAGCT GGTGGATTTT 
GGATCAGCCG CTAAGATGAA TTCAAATAAG GTGGATGCCA AACTCCCCAT TGGGACCCCG 
GATTACATGG CTCCGGAAGT GTTGACCGTG ATGAACGAGG ACCGAAGGGG CACATACGGC 
TTGGACTGTG ACTGGTGGTC TGTCGGAGTT GTTGCTTATG AGATGGTTTA TGGGAAGACC 
CCATTCACAG AGGGAACCTC CGCCCGGACC TTCAACAACA TCATGAACTT CCAGCGGTTT 
TTGAAGTTCC CAGATGACCC CAAAGTTAGC AGTGAGCTCC TTGATCTGCT TCAGAGTCTG 
CTGTGTGTCC AGAAAGAGAG ACTGAAGTTC GAGGGTCTCT GCTGCCACCC TTTCTTTGCC 
AGAACGGACT GGAACAACAT CCGTAACTCT CCTCCCCCCT TCGTCCCCAC CCTCAAGTCT 
GACGATGACA CCTCCAATTT TGATGAACCA GAGAAGAATT CGTGGGTTTC ATCCTCTGTG 
TGCCAGCTGA GCCCCTCGGG CTTCTCAGGC GAAGAGCTGC CGTTTGTGGG ATTTTCGTAC 
AGCAAGGCAC TGGGGTATCT TGGTAGATCT GAGTCTGTCG TGTCGAGTCT GGACTCCCCT 
GCCAAGGTTA GCTCCATGGA AAAGAAACTT CTCATCAAAA GCAAAGAGCT CCAAGACTCC 
CAGGACAAGT GTCACAAGAT GGAGCAGGAA ATGACCCGGT TACATCGCAG AGTGTCAGAG 
GTGGAGGCTG TGCTTAGTCA GAAGGAGGTG GAGCTGAAGG CCTCTGAGAC TCAGAGATCC 
CTCCTGGAGC AGGACCTTGC TACCTACATC ACAGAATGCA GTAGCTTAAA GCGAAGTTTG 
GAGCAAGCGC GGATGGAGGT GTCCCAGGAG GATGACAAAG CTCTGCAGCT TCTCCACGAC 
ATCCGAGAGC AGAGCCGGAA GCTCCAGGAG ATCAAGGAGC AGGAGTACCA GGCTCAGGTG 
GAGGAGATGA GGCTGATGAT GAATCAGCTG GAAGAAGACC TGGTGTCAGC CCGCAGACGC 
AGCGATCTCT ACGAGTCTGA GCTGAGGGAG TCTCGGCTTG CCGCCGAGGA ATTCAAGCGG 
AAGGCAAACG AATGTCAGCA CAAACTGATG AAGGCTAAGG ACCAAGGGAA GCCTGAAGTG 
GGAGAATATT CCAAACTGGA GAAGATCAAT GCTGAGCAGC AGCTGAAGAT CCAGGAGCTC 
CAGGAGAAGC TGGAGAAGGC TGTAAAAGCC AGCACAGAGG CCACCGAGCT CCTGCAGAAT 
ATCCGCCAGG CAAAGGAGCG AGCAGAGCGG GAGCTAGAGA AGCTACACAA CCGGGAAGAC 
TCCTCCGAGG GCATCAAAAA GAAGCTGGTG GAAGCCGAGG AGCTGGAGGA GAAACATCGG 
GAGGCTCAGG TCTCAGCTCA ACATCTAGAA GTACACTTGA AACAGAAAGA ACAGCACTAC 
GAGGAAAAGA TCAAGGTATT GGACAATCAG ATAAAGAAGG ACCTGGCCGA CAAGGAGAGC 
CTGGAGAACA TGATGCAGAG ACACGAGGAG GAGGCCCACG AGAAGGGCAA GATCCTCAGC 
GAGCAGAAGG CGATGATCAA CGCGATGGAT TCCAAGATCC GATCCCTGGA GCAGAGGATC 
GTGGAGCTGT CGGAAGCCAA CAAGCTTGCG GCAAACAGCA GTCTCTTCAC CCAGAGGAAC 
ATGAAGGCCC AGGAAGAGAT GATCTCAGAA CTCAGGCAGC AGAAATTTTA CCTGGAGACG 
CAGGCCGGAA AGCTGGAGGC CCAGAACCGA AAGCTGGAAG AGCAACTGGA GAAGATCAGC 
CACCAAGATC ACAGTGACAA GAGTCGGCTG CTGGAGCTGG AAACAAGGCT GAGGGAGGTC 
AGCCTGGAGC ACGAGGAGCA GAAGCTGGAG CTGAAGCGGC AGCTCACGGA GCTGCAGCTG 
TCCCTGCAGG AGCGCGAGTC CCAGCTGACG GCCCTGCAGG CCGCCCGGGC AGCGCTGGAG 
AGCCAGCTCC GCCAGGCGAA GACAGAGCTG GAGGAGACAA CCGCGGAAGC GGAGGAGGAG 
ATCCAGGCGC TCACGGCACA TCGAGATGAG ATCCAGCGCA AATTCGATGC CCTTCGCAAC 
AGCTGCACGG TCATCACCGA CCTGGAGGAG CAGCTGAACC AGCTCACCGA GGACAACGCC 
GAGCTCAACA ACCAAAACTT CTACCTGTCC AAACAACTCG ATGAGGCTTC CGGGGCCAAT 
GACGAGATTG TGCAGCTGCG GAGTGAGGTG GACCATCTCC GCCGTGAGAT CACGGAGCGG 
GAGATGCAGC TCACCAGCCA GAAGCAAACG ATGGAGGCTC TGAAGACGAC ATGCACCATG 
CTGGAGGAGC AGGTCCTGGA CCTGGAGGCC CTGAACGACG AGCTGCTGGA GAAGGAGCGC 
CAGTGGGAGG CCTGGCGGAG CGTCCTCGGC GACGAGAAGT CCCAGTTCGA GTGTCGAGTT 
CGAGAGCTAC AGAGGATGCT GGACACCGAG AAGCAGAGCA GGGCTAGGGC CGATCAGCGG 
ATCACCGAGT CGCGCCAGGT GGTGGAGTTG GCGGTGAAGG AACACAAGGC CGAGATTCTT 
GCTCTGCAGC AGGCTCTCAA GGAGCAGAAG CTCAAAGCCG AGAGCCTGTC GGACAAGCTC 
AACGACCTGG AGAAGAAGCA CGCCATGCTG GAGATGAACG CCCGGAGCTT ACAGCAGAAA 
CTAGAGACAG AGCGGGAGCT CAAACAGAGG CTTCTGGAGG AGCAAGCCAA ATTGCAGCAG 
CAGATGGACC TGCAGAAGAA CCACATCTTC AGACTGACGC AAGGGCTGCA GGAGGCGCTG 
GACCGGGCGG ATCTGCTGAA GACAGAAAGG AGCGACCTGG AGTACCAGCT GGAAAACATT 
CAGGTTCTCT ACTCTCACGA GAAAGTGAAA ATGGAAGGCA CAATCTCTCA GCAAACCAAA 
CTCATTGATT TCCTGCAAGC CAAAATGGAC CAGCCTGCTA AAAAGAAAAA GGTTCCTCTG 
CAGTACAATG AGCTGAAGCT AGCCCTGGAA AAGGAGAAAG CCCGATGCGC GGAGCTGGAG 
GAGGCCCTTC AGAAGACCCG CATCGAACTC CGCTCTGCGC GGGAGGAAGC TGCCCACCGC 
AAAGCCACGG ACCACCCGCA CCCGTCTACG CCAGCCACTG CGAGGCAGCA GATCGCCATG 
TCTGCCATTG TGCGGTCGCC CGAGCACCAG CCCAGTGCCA TGAGCCTGCT TGCCCCACCA 
TCCAGCCGCA GAAAGGAGTC GTCAACTCCA GAGGAATTCA GCCGGCGTCT GAAAGAGCGC 
ATGCACCACA ACATCCCTCA CCGGTTTAAT GTGGGCCTGA ACATGAGAGC CACCAAGTGC 
GCCGTGTGTC TGGATACTGT GCACTTTGGA CGCCAGGCAT CCAAATGCCT CGAATGTCAA 
GTGATGTGTC ACCCCAAATG CTCCACCTGC TTGCCCGCTA CCTGTGGCCT GCCAGCTGAA 
TATGCCACAC ACTTCACTGA GGCCTTCTGC CGGGACAAAA TGAACTCCCC GGGGCTCCAG 
AGCAAGGAGC CTGGCAGCAG CTTGCACCTG GAAGGGTGGA TGAAAGTGCC CAGAAATAAC 
AAACGGGGAC AGCAAGGCTG GGACAGGAAG TACATTGTTC TGGAGGGGTC AAAAGTCCTC 
ATCTATGACA ATGAAGCCAG AGAAGCTGGA CAGAGGCCGG TGGAAGAATT TGAGCTGTGC 
CTTCCTGACG GGGACGTATC TATTCATGGT GCCGTTGGTG CTTCAGAACT TGCAAATACC 
GCCAAAGCAG ATGTCCCATA CATCCTGAAG ATGGAGTCTC ACCCACACAC CACCTGCTGG 
CCTGGGAGAA CCCTCTACTT GCTAGCACCC AGCTTCCCCG ACAAGCAGCG CTGGGTCACC 
GCCTTAGAAT CTGTCGTCGC AGGTGGGAGA GTTTCTAGGG AAAAGGCCGA AGCCGATGCT 
AAATTACTTG GAAACTCTCT GCTGAAACTG GAGGGCGATG ACCGGCTTGA CATGAACTGC 
ACCCTGCCCT TCAGTGACCA GGTAGTGCTG GTGGGCACGG AGGAAGGCCT CTACGCCCTG 
AATGTCTTGA AAAACTCCTT AACCCACATC CCAGGGATTG GCGCAGTCTT CCAAATTTAC 
ATCATCAAGG ACCTGGAGAA GCTGCTCATG ATAGCAGGGG AAGAGCGGGC TCTGTGCCTG 
GTGGACGTGA AGAAGGTGAA GCAGTCCCTG GCGCAGTCAC ACCTGCCTGC CCAGCCCGAC 
GTCTCCCCCA ACATATTCGA AGCCGTCAAA GGCTGCCACT TGTTCGCTGC TGGCAAGATC 
GAGAACAGCC TGTGCATCTG CGCCGCTATG CCAAGCAAAG TCGTCATCCT CCGCTACAAT 
GACAACCTCA GCAAGTACTG CATCCGCAAG GAGATCGAGA CCTCAGAGCC CTGCAGCTGT 
ATCCACTTCA CCAACTACAG CATCCTCATT GGGACCAACA AATTCTATGA GATCGACATG 
AAGCAGTACA CGCTTGACGA GTTCCTGGAC AAGAACGACC ATTCCTTGGC ACCTGCTGTG 
TTCGCCTCCT CGTCCAACAG CTTCCCTGTC TCCATTGTGC AGGCGAACAG CGCCGGGCAG 
CGAGAAGAAT ACCTGCTGTG CTTCCACGAA TTTGGGGTGT TCGTGGATTC TTACGGAAGA 
CGTAGCCGCA CAGATGATCT CAAGTGGAGT CGCTTACCTC TGGCCTTCGC CTACAGAGAA 
CCTTATCTGT TTGTGACTCA CTTCAACTCC CTGGAAGTCA TTGAGATCCA GGCCAGATCC 
TCACTGGGGA GCCCTGCCCG AGCATATCTG GAAATTCCAA ACCCTCGCTA CCTGGGCCCC 
GCGATTTCCT CCGGAGCGAT TTACCTGGCC TCCTCATACC AGGACAAGTT AAGGGTCATA 
TGCTGCAAAG GAAACCTCGT GAAGGAGTCA GGCACTGAGC AGCACCGGGT GCCCTCCACC 
TCCCGCAGCA GCCCCAACAA GCGAGGCCCA CCAACATACA ACGAGCACAT CACCAAACGC 
GTGGCCTCCA GCCCGGCGCC ACCGGAAGGC CCCAGCCACC CCCGAGAGCC AAGCACACCG 
CACCGCTACC GAGACAGAGA GGGTCGGACA GAGCTGCGCA GGGACAAGTC TCCAGGCCGC 
CCTCTGGAGC GGGAGAAGTC CCCAGGCCGA ATGCTCAGCA CTAGGAGAGA GCGGTCCCCA 
GGGAGACTGT TTGAAGACAG CAGCAGGGGC CGGCTGCCTG CAGGAGCAGT GAGGACCCCA 
CTGTCCCAGG TTAACAAGGT CTGGGACCAG TCTTCAGTAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006530208.1
CDS	157..6198
Translation	MLKFKYGVRNPPEASASEPIASRASRLNLFFQGKPPLMTQQQMSALSREGMLDALFALFEECSQPALMKMKHVSSFVQKYSDTIAELRELQPSARDFEVRSLVGCGHFAEVQVVREKATGDVYAMKIMKKKALLAQEQVSFFEEERNILSRSTSPWIPQLQYAFQDKNNLYLVMEYQPGGDLLSLLNRYEDQLDESMIQFYLAELILAVHSVHQMGYVHRDIKPENILIDRTGHIKLVDFGSAAKMNSNKVDAKLPIGTPDYMAPEVLTVMNEDRRGTYGLDCDWWSVGVVAYEMVYGKTPFTEGTSARTFNNIMNFQRFLKFPDDPKVSSELLDLLQSLLCVQKERLKFEGLCCHPFFARTDWNNIRNSPPPFVPTLKSDDDTSNFDEPEKNSWVSSSVCQLSPSGFSGEELPFVGFSYSKALGYLGRSESVVSSLDSPAKVSSMEKKLLIKSKELQDSQDKCHKMEQEMTRLHRRVSEVEAVLSQKEVELKASETQRSLLEQDLATYITECSSLKRSLEQARMEVSQEDDKALQLLHDIREQSRKLQEIKEQEYQAQVEEMRLMMNQLEEDLVSARRRSDLYESELRESRLAAEEFKRKANECQHKLMKAKDQGKPEVGEYSKLEKINAEQQLKIQELQEKLEKAVKASTEATELLQNIRQAKERAERELEKLHNREDSSEGIKKKLVEAEELEEKHREAQVSAQHLEVHLKQKEQHYEEKIKVLDNQIKKDLADKESLENMMQRHEEEAHEKGKILSEQKAMINAMDSKIRSLEQRIVELSEANKLAANSSLFTQRNMKAQEEMISELRQQKFYLETQAGKLEAQNRKLEEQLEKISHQDHSDKSRLLELETRLREVSLEHEEQKLELKRQLTELQLSLQERESQLTALQAARAALESQLRQAKTELEETTAEAEEEIQALTAHRDEIQRKFDALRNSCTVITDLEEQLNQLTEDNAELNNQNFYLSKQLDEASGANDEIVQLRSEVDHLRREITEREMQLTSQKQTMEALKTTCTMLEEQVLDLEALNDELLEKERQWEAWRSVLGDEKSQFECRVRELQRMLDTEKQSRARADQRITESRQVVELAVKEHKAEILALQQALKEQKLKAESLSDKLNDLEKKHAMLEMNARSLQQKLETERELKQRLLEEQAKLQQQMDLQKNHIFRLTQGLQEALDRADLLKTERSDLEYQLENIQVLYSHEKVKMEGTISQQTKLIDFLQAKMDQPAKKKKVPLQYNELKLALEKEKARCAELEEALQKTRIELRSAREEAAHRKATDHPHPSTPATARQQIAMSAIVRSPEHQPSAMSLLAPPSSRRKESSTPEEFSRRLKERMHHNIPHRFNVGLNMRATKCAVCLDTVHFGRQASKCLECQVMCHPKCSTCLPATCGLPAEYATHFTEAFCRDKMNSPGLQSKEPGSSLHLEGWMKVPRNNKRGQQGWDRKYIVLEGSKVLIYDNEAREAGQRPVEEFELCLPDGDVSIHGAVGASELANTAKADVPYILKMESHPHTTCWPGRTLYLLAPSFPDKQRWVTALESVVAGGRVSREKAEADAKLLGNSLLKLEGDDRLDMNCTLPFSDQVVLVGTEEGLYALNVLKNSLTHIPGIGAVFQIYIIKDLEKLLMIAGEERALCLVDVKKVKQSLAQSHLPAQPDVSPNIFEAVKGCHLFAAGKIENSLCICAAMPSKVVILRYNDNLSKYCIRKEIETSEPCSCIHFTNYSILIGTNKFYEIDMKQYTLDEFLDKNDHSLAPAVFASSSNSFPVSIVQANSAGQREEYLLCFHEFGVFVDSYGRRSRTDDLKWSRLPLAFAYREPYLFVTHFNSLEVIEIQARSSLGSPARAYLEIPNPRYLGPAISSGAIYLASSYQDKLRVICCKGNLVKESGTEQHRVPSTSRSSPNKRGPPTYNEHITKRVASSPAPPEGPSHPREPSTPHRYRDREGRTELRRDKSPGRPLEREKSPGRMLSTRRERSPGRLFEDSSRGRLPAGAVRTPLSQVNKVWDQSSV

Target ORF information:

RefSeq Version	XM_006530145.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus citron (Cit), transcript variant X19, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006530145.4

ORF Insert Sequence:

ATGTTGAAGT TCAAGTATGG TGTGCGGAAC CCGCCGGAGG CCAGTGCCTC CGAGCCCATT 
GCCAGTCGGG CCTCCAGGCT AAATCTCTTC TTCCAGGGGA AACCGCCCCT CATGACTCAA 
CAGCAGATGT CTGCTCTTTC CCGGGAAGGG ATGCTAGACG CCCTCTTCGC TCTCTTTGAA 
GAGTGCAGCC AACCCGCCCT GATGAAGATG AAGCACGTGA GCAGCTTTGT CCAGAAGTAT 
TCCGACACCA TAGCCGAGTT GCGGGAGCTG CAGCCGTCGG CGAGAGACTT CGAAGTTCGA 
AGCCTTGTGG GCTGTGGTCA CTTCGCTGAA GTGCAGGTGG TTAGAGAGAA GGCGACCGGG 
GACGTCTATG CCATGAAAAT CATGAAGAAG AAGGCTTTGC TGGCCCAGGA ACAGGTTTCA 
TTTTTCGAGG AGGAGAGGAA CATATTATCT CGGAGCACGA GTCCTTGGAT CCCCCAGTTA 
CAGTACGCCT TTCAGGACAA AAATAACCTT TACCTGGTCA TGGAATATCA GCCTGGAGGG 
GATTTGCTGT CGCTTCTGAA CAGATACGAG GACCAATTAG ATGAGAGCAT GATCCAGTTT 
TACCTTGCTG AGCTGATTTT GGCTGTCCAC AGCGTGCACC AGATGGGATA TGTGCATCGA 
GACATCAAGC CCGAGAACAT CCTCATCGAC CGGACGGGAC ACATCAAGCT GGTGGATTTT 
GGATCAGCCG CTAAGATGAA TTCAAATAAG GTGGATGCCA AACTCCCCAT TGGGACCCCG 
GATTACATGG CTCCGGAAGT GTTGACCGTG ATGAACGAGG ACCGAAGGGG CACATACGGC 
TTGGACTGTG ACTGGTGGTC TGTCGGAGTT GTTGCTTATG AGATGGTTTA TGGGAAGACC 
CCATTCACAG AGGGAACCTC CGCCCGGACC TTCAACAACA TCATGAACTT CCAGCGGTTT 
TTGAAGTTCC CAGATGACCC CAAAGTTAGC AGTGAGCTCC TTGATCTGCT TCAGAGTCTG 
CTGTGTGTCC AGAAAGAGAG ACTGAAGTTC GAGGGTCTCT GCTGCCACCC TTTCTTTGCC 
AGAACGGACT GGAACAACAT CCGTAACTCT CCTCCCCCCT TCGTCCCCAC CCTCAAGTCT 
GACGATGACA CCTCCAATTT TGATGAACCA GAGAAGAATT CGTGGGTTTC ATCCTCTGTG 
TGCCAGCTGA GCCCCTCGGG CTTCTCAGGC GAAGAGCTGC CGTTTGTGGG ATTTTCGTAC 
AGCAAGGCAC TGGGGTATCT TGGTAGATCT GAGTCTGTCG TGTCGAGTCT GGACTCCCCT 
GCCAAGGTTA GCTCCATGGA AAAGAAACTT CTCATCAAAA GCAAAGAGCT CCAAGACTCC 
CAGGACAAGT GTCACAAGAT GGAGCAGGAA ATGACCCGGT TACATCGCAG AGTGTCAGAG 
GTGGAGGCTG TGCTTAGTCA GAAGGAGGTG GAGCTGAAGG CCTCTGAGAC TCAGAGATCC 
CTCCTGGAGC AGGACCTTGC TACCTACATC ACAGAATGCA GTAGCTTAAA GCGAAGTTTG 
GAGCAAGCGC GGATGGAGGT GTCCCAGGAG GATGACAAAG CTCTGCAGCT TCTCCACGAC 
ATCCGAGAGC AGAGCCGGAA GCTCCAGGAG ATCAAGGAGC AGGAGTACCA GGCTCAGGTG 
GAGGAGATGA GGCTGATGAT GAATCAGCTG GAAGAAGACC TGGTGTCAGC CCGCAGACGC 
AGCGATCTCT ACGAGTCTGA GCTGAGGGAG TCTCGGCTTG CCGCCGAGGA ATTCAAGCGG 
AAGGCAAACG AATGTCAGCA CAAACTGATG AAGGCTAAGG ACCAAGGGAA GCCTGAAGTG 
GGAGAATATT CCAAACTGGA GAAGATCAAT GCTGAGCAGC AGCTGAAGAT CCAGGAGCTC 
CAGGAGAAGC TGGAGAAGGC TGTAAAAGCC AGCACAGAGG CCACCGAGCT CCTGCAGAAT 
ATCCGCCAGG CAAAGGAGCG AGCAGAGCGG GAGCTAGAGA AGCTACACAA CCGGGAAGAC 
TCCTCCGAGG GCATCAAAAA GAAGCTGGTG GAAGCCGAGG AGCTGGAGGA GAAACATCGG 
GAGGCTCAGG TCTCAGCTCA ACATCTAGAA GTACACTTGA AACAGAAAGA ACAGCACTAC 
GAGGAAAAGA TCAAGGTATT GGACAATCAG ATAAAGAAGG ACCTGGCCGA CAAGGAGAGC 
CTGGAGAACA TGATGCAGAG ACACGAGGAG GAGGCCCACG AGAAGGGCAA GATCCTCAGC 
GAGCAGAAGG CGATGATCAA CGCGATGGAT TCCAAGATCC GATCCCTGGA GCAGAGGATC 
GTGGAGCTGT CGGAAGCCAA CAAGCTTGCG GCAAACAGCA GTCTCTTCAC CCAGAGGAAC 
ATGAAGGCCC AGGAAGAGAT GATCTCAGAA CTCAGGCAGC AGAAATTTTA CCTGGAGACG 
CAGGCCGGAA AGCTGGAGGC CCAGAACCGA AAGCTGGAAG AGCAACTGGA GAAGATCAGC 
CACCAAGATC ACAGTGACAA GAGTCGGCTG CTGGAGCTGG AAACAAGGCT GAGGGAGGTC 
AGCCTGGAGC ACGAGGAGCA GAAGCTGGAG CTGAAGCGGC AGCTCACGGA GCTGCAGCTG 
TCCCTGCAGG AGCGCGAGTC CCAGCTGACG GCCCTGCAGG CCGCCCGGGC AGCGCTGGAG 
AGCCAGCTCC GCCAGGCGAA GACAGAGCTG GAGGAGACAA CCGCGGAAGC GGAGGAGGAG 
ATCCAGGCGC TCACGGCACA TCGAGATGAG ATCCAGCGCA AATTCGATGC CCTTCGCAAC 
AGCTGCACGG TCATCACCGA CCTGGAGGAG CAGCTGAACC AGCTCACCGA GGACAACGCC 
GAGCTCAACA ACCAAAACTT CTACCTGTCC AAACAACTCG ATGAGGCTTC CGGGGCCAAT 
GACGAGATTG TGCAGCTGCG GAGTGAGGTG GACCATCTCC GCCGTGAGAT CACGGAGCGG 
GAGATGCAGC TCACCAGCCA GAAGCAAACG ATGGAGGCTC TGAAGACGAC ATGCACCATG 
CTGGAGGAGC AGGTCCTGGA CCTGGAGGCC CTGAACGACG AGCTGCTGGA GAAGGAGCGC 
CAGTGGGAGG CCTGGCGGAG CGTCCTCGGC GACGAGAAGT CCCAGTTCGA GTGTCGAGTT 
CGAGAGCTAC AGAGGATGCT GGACACCGAG AAGCAGAGCA GGGCTAGGGC CGATCAGCGG 
ATCACCGAGT CGCGCCAGGT GGTGGAGTTG GCGGTGAAGG AACACAAGGC CGAGATTCTT 
GCTCTGCAGC AGGCTCTCAA GGAGCAGAAG CTCAAAGCCG AGAGCCTGTC GGACAAGCTC 
AACGACCTGG AGAAGAAGCA CGCCATGCTG GAGATGAACG CCCGGAGCTT ACAGCAGAAA 
CTAGAGACAG AGCGGGAGCT CAAACAGAGG CTTCTGGAGG AGCAAGCCAA ATTGCAGCAG 
CAGATGGACC TGCAGAAGAA CCACATCTTC AGACTGACGC AAGGGCTGCA GGAGGCGCTG 
GACCGGGCGG ATCTGCTGAA GACAGAAAGG AGCGACCTGG AGTACCAGCT GGAAAACATT 
CAGGTTCTCT ACTCTCACGA GAAAGTGAAA ATGGAAGGCA CAATCTCTCA GCAAACCAAA 
CTCATTGATT TCCTGCAAGC CAAAATGGAC CAGCCTGCTA AAAAGAAAAA GGTTCCTCTG 
CAGTACAATG AGCTGAAGCT AGCCCTGGAA AAGGAGAAAG CCCGATGCGC GGAGCTGGAG 
GAGGCCCTTC AGAAGACCCG CATCGAACTC CGCTCTGCGC GGGAGGAAGC TGCCCACCGC 
AAAGCCACGG ACCACCCGCA CCCGTCTACG CCAGCCACTG CGAGGCAGCA GATCGCCATG 
TCTGCCATTG TGCGGTCGCC CGAGCACCAG CCCAGTGCCA TGAGCCTGCT TGCCCCACCA 
TCCAGCCGCA GAAAGGAGTC GTCAACTCCA GAGGAATTCA GCCGGCGTCT GAAAGAGCGC 
ATGCACCACA ACATCCCTCA CCGGTTTAAT GTGGGCCTGA ACATGAGAGC CACCAAGTGC 
GCCGTGTGTC TGGATACTGT GCACTTTGGA CGCCAGGCAT CCAAATGCCT CGAATGTCAA 
GTGATGTGTC ACCCCAAATG CTCCACCTGC TTGCCCGCTA CCTGTGGCCT GCCAGCTGAA 
TATGCCACAC ACTTCACTGA GGCCTTCTGC CGGGACAAAA TGAACTCCCC GGGGCTCCAG 
AGCAAGGAGC CTGGCAGCAG CTTGCACCTG GAAGGGTGGA TGAAAGTGCC CAGAAATAAC 
AAACGGGGAC AGCAAGGCTG GGACAGGAAG TACATTGTTC TGGAGGGGTC AAAAGTCCTC 
ATCTATGACA ATGAAGCCAG AGAAGCTGGA CAGAGGCCGG TGGAAGAATT TGAGCTGTGC 
CTTCCTGACG GGGACGTATC TATTCATGGT GCCGTTGGTG CTTCAGAACT TGCAAATACC 
GCCAAAGCAG ATGTCCCATA CATCCTGAAG ATGGAGTCTC ACCCACACAC CACCTGCTGG 
CCTGGGAGAA CCCTCTACTT GCTAGCACCC AGCTTCCCCG ACAAGCAGCG CTGGGTCACC 
GCCTTAGAAT CTGTCGTCGC AGGTGGGAGA GTTTCTAGGG AAAAGGCCGA AGCCGATGCT 
AAATTACTTG GAAACTCTCT GCTGAAACTG GAGGGCGATG ACCGGCTTGA CATGAACTGC 
ACCCTGCCCT TCAGTGACCA GGTAGTGCTG GTGGGCACGG AGGAAGGCCT CTACGCCCTG 
AATGTCTTGA AAAACTCCTT AACCCACATC CCAGGGATTG GCGCAGTCTT CCAAATTTAC 
ATCATCAAGG ACCTGGAGAA GCTGCTCATG ATAGCAGGGG AAGAGCGGGC TCTGTGCCTG 
GTGGACGTGA AGAAGGTGAA GCAGTCCCTG GCGCAGTCAC ACCTGCCTGC CCAGCCCGAC 
GTCTCCCCCA ACATATTCGA AGCCGTCAAA GGCTGCCACT TGTTCGCTGC TGGCAAGATC 
GAGAACAGCC TGTGCATCTG CGCCGCTATG CCAAGCAAAG TCGTCATCCT CCGCTACAAT 
GACAACCTCA GCAAGTACTG CATCCGCAAG GAGATCGAGA CCTCAGAGCC CTGCAGCTGT 
ATCCACTTCA CCAACTACAG CATCCTCATT GGGACCAACA AATTCTATGA GATCGACATG 
AAGCAGTACA CGCTTGACGA GTTCCTGGAC AAGAACGACC ATTCCTTGGC ACCTGCTGTG 
TTCGCCTCCT CGTCCAACAG CTTCCCTGTC TCCATTGTGC AGGCGAACAG CGCCGGGCAG 
CGAGAAGAAT ACCTGCTGTG CTTCCACGAA TTTGGGGTGT TCGTGGATTC TTACGGAAGA 
CGTAGCCGCA CAGATGATCT CAAGTGGAGT CGCTTACCTC TGGCCTTCGC CTACAGAGAA 
CCTTATCTGT TTGTGACTCA CTTCAACTCC CTGGAAGTCA TTGAGATCCA GGCCAGATCC 
TCACTGGGGA GCCCTGCCCG AGCATATCTG GAAATTCCAA ACCCTCGCTA CCTGGGCCCC 
GCGATTTCCT CCGGAGCGAT TTACCTGGCC TCCTCATACC AGGACAAGTT AAGGGTCATA 
TGCTGCAAAG GAAACCTCGT GAAGGAGTCA GGCACTGAGC AGCACCGGGT GCCCTCCACC 
TCCCGCAGCA GCCCCAACAA GCGAGGCCCA CCAACATACA ACGAGCACAT CACCAAACGC 
GTGGCCTCCA GCCCGGCGCC ACCGGAAGGC CCCAGCCACC CCCGAGAGCC AAGCACACCG 
CACCGCTACC GAGACAGAGA GGGTCGGACA GAGCTGCGCA GGGACAAGTC TCCAGGCCGC 
CCTCTGGAGC GGGAGAAGTC CCCAGGCCGA ATGCTCAGCA CTAGGAGAGA GCGGTCCCCA 
GGGAGACTGT TTGAAGACAG CAGCAGGGGC CGGCTGCCTG CAGGAGCAGT GAGGACCCCA 
CTGTCCCAGG TTAACAAGGT CTGGGACCAG TCTTCAGTAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cit ( XM_011248163.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011246465.1 Mus musculus citron (Cit), transcript variant X20, mRNA.

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CloneID	OMu67373
Clone ID Related Accession (Same CDS sequence)	XM_011248163.2 , XM_011248163.3
Accession Version	XM_011248163.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6039bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	citron Rho-interacting kinase isoform X16
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000071.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_011248163.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTGAAGT TCAAGTATGG TGTGCGGAAC CCGCCGGAGG CCAGTGCCTC CGAGCCCATT 
GCCAGTCGGG CCTCCAGGCT AAATCTCTTC TTCCAGGGGA AACCGCCCCT CATGACTCAA 
CAGCAGATGT CTGCTCTTTC CCGGGAAGGG ATGCTAGACG CCCTCTTCGC TCTCTTTGAA 
GAGTGCAGCC AACCCGCCCT GATGAAGATG AAGCACGTGA GCAGCTTTGT CCAGAAGTAT 
TCCGACACCA TAGCCGAGTT GCGGGAGCTG CAGCCGTCGG CGAGAGACTT CGAAGTTCGA 
AGCCTTGTGG GCTGTGGTCA CTTCGCTGAA GTGCAGGTGG TTAGAGAGAA GGCGACCGGG 
GACGTCTATG CCATGAAAAT CATGAAGAAG AAGGCTTTGC TGGCCCAGGA ACAGGTTTCA 
TTTTTCGAGG AGGAGAGGAA CATATTATCT CGGAGCACGA GTCCTTGGAT CCCCCAGTTA 
CAGTACGCCT TTCAGGACAA AAATAACCTT TACCTGGTCA TGGAATATCA GCCTGGAGGG 
GATTTGCTGT CGCTTCTGAA CAGATACGAG GACCAATTAG ATGAGAGCAT GATCCAGTTT 
TACCTTGCTG AGCTGATTTT GGCTGTCCAC AGCGTGCACC AGATGGGATA TGTGCATCGA 
GACATCAAGC CCGAGAACAT CCTCATCGAC CGGACGGGAC ACATCAAGCT GGTGGATTTT 
GGATCAGCCG CTAAGATGAA TTCAAATAAG GTGGATGCCA AACTCCCCAT TGGGACCCCG 
GATTACATGG CTCCGGAAGT GTTGACCGTG ATGAACGAGG ACCGAAGGGG CACATACGGC 
TTGGACTGTG ACTGGTGGTC TGTCGGAGTT GTTGCTTATG AGATGGTTTA TGGGAAGACC 
CCATTCACAG AGGGAACCTC CGCCCGGACC TTCAACAACA TCATGAACTT CCAGCGGTTT 
TTGAAGTTCC CAGATGACCC CAAAGTTAGC AGTGAGCTCC TTGATCTGCT TCAGAGTCTG 
CTGTGTGTCC AGAAAGAGAG ACTGAAGTTC GAGGGTCTCT GCTGCCACCC TTTCTTTGCC 
AGAACGGACT GGAACAACAT CCGTAACTCT CCTCCCCCCT TCGTCCCCAC CCTCAAGTCT 
GACGATGACA CCTCCAATTT TGATGAACCA GAGAAGAATT CGTGGGTTTC ATCCTCTGTG 
TGCCAGCTGA GCCCCTCGGG CTTCTCAGGC GAAGAGCTGC CGTTTGTGGG ATTTTCGTAC 
AGCAAGGCAC TGGGGTATCT TGGTAGATCT GAGTCTGTCG TGTCGAGTCT GGACTCCCCT 
GCCAAGGTTA GCTCCATGGA AAAGAAACTT CTCATCAAAA GCAAAGAGCT CCAAGACTCC 
CAGGACAAGT GTCACAAGAT GGAGCAGGAA ATGACCCGGT TACATCGCAG AGTGTCAGAG 
GTGGAGGCTG TGCTTAGTCA GAAGGAGGTG GAGCTGAAGG CCTCTGAGAC TCAGAGATCC 
CTCCTGGAGC AGGACCTTGC TACCTACATC ACAGAATGCA GTAGCTTAAA GCGAAGTTTG 
GAGCAAGCGC GGATGGAGGT GTCCCAGGAG GATGACAAAG CTCTGCAGCT TCTCCACGAC 
ATCCGAGAGC AGAGCCGGAA GCTCCAGGAG ATCAAGGAGC AGGAGTACCA GGCTCAGGTG 
GAGGAGATGA GGCTGATGAT GAATCAGCTG GAAGAAGACC TGGTGTCAGC CCGCAGACGC 
AGCGATCTCT ACGAGTCTGA GCTGAGGGAG TCTCGGCTTG CCGCCGAGGA ATTCAAGCGG 
AAGGCAAACG AATGTCAGCA CAAACTGATG AAGGCTAAGG ACCAAGGGAA GCCTGAAGTG 
GGAGAATATT CCAAACTGGA GAAGATCAAT GCTGAGCAGC AGCTGAAGAT CCAGGAGCTC 
CAGGAGAAGC TGGAGAAGGC TGTAAAAGCC AGCACAGAGG CCACCGAGCT CCTGCAGAAT 
ATCCGCCAGG CAAAGGAGCG AGCAGAGCGG GAGCTAGAGA AGCTACACAA CCGGGAAGAC 
TCCTCCGAGG GCATCAAAAA GAAGCTGGTG GAAGCCGAGG AGCTGGAGGA GAAACATCGG 
GAGGCTCAGG TCTCAGCTCA ACATCTAGAA GTACACTTGA AACAGAAAGA ACAGCACTAC 
GAGGAAAAGA TCAAGGTATT GGACAATCAG ATAAAGAAGG ACCTGGCCGA CAAGGAGAGC 
CTGGAGAACA TGATGCAGAG ACACGAGGAG GAGGCCCACG AGAAGGGCAA GATCCTCAGC 
GAGCAGAAGG CGATGATCAA CGCGATGGAT TCCAAGATCC GATCCCTGGA GCAGAGGATC 
GTGGAGCTGT CGGAAGCCAA CAAGCTTGCG GCAAACAGCA GTCTCTTCAC CCAGAGGAAC 
ATGAAGGCCC AGGAAGAGAT GATCTCAGAA CTCAGGCAGC AGAAATTTTA CCTGGAGACG 
CAGGCCGGAA AGCTGGAGGC CCAGAACCGA AAGCTGGAAG AGCAACTGGA GAAGATCAGC 
CACCAAGATC ACAGTGACAA GAGTCGGCTG CTGGAGCTGG AAACAAGGCT GAGGGAGGTC 
AGCCTGGAGC ACGAGGAGCA GAAGCTGGAG CTGAAGCGGC AGCTCACGGA GCTGCAGCTG 
TCCCTGCAGG AGCGCGAGTC CCAGCTGACG GCCCTGCAGG CCGCCCGGGC AGCGCTGGAG 
AGCCAGCTCC GCCAGGCGAA GACAGAGCTG GAGGAGACAA CCGCGGAAGC GGAGGAGGAG 
ATCCAGGCGC TCACGGCACA TCGAGATGAG ATCCAGCGCA AATTCGATGC CCTTCGCAAC 
AGCTGCACGG TCATCACCGA CCTGGAGGAG CAGCTGAACC AGCTCACCGA GGACAACGCC 
GAGCTCAACA ACCAAAACTT CTACCTGTCC AAACAACTCG ATGAGGCTTC CGGGGCCAAT 
GACGAGATTG TGCAGCTGCG GAGTGAGGTG GACCATCTCC GCCGTGAGAT CACGGAGCGG 
GAGATGCAGC TCACCAGCCA GAAGCAAACG ATGGAGGCTC TGAAGACGAC ATGCACCATG 
CTGGAGGAGC AGGTCCTGGA CCTGGAGGCC CTGAACGACG AGCTGCTGGA GAAGGAGCGC 
CAGTGGGAGG CCTGGCGGAG CGTCCTCGGC GACGAGAAGT CCCAGTTCGA GTGTCGAGTT 
CGAGAGCTAC AGAGGATGCT GGACACCGAG AAGCAGAGCA GGGCTAGGGC CGATCAGCGG 
ATCACCGAGT CGCGCCAGGT GGTGGAGTTG GCGGTGAAGG AACACAAGGC CGAGATTCTT 
GCTCTGCAGC AGGCTCTCAA GGAGCAGAAG CTCAAAGCCG AGAGCCTGTC GGACAAGCTC 
AACGACCTGG AGAAGAAGCA CGCCATGCTG GAGATGAACG CCCGGAGCTT ACAGCAGAAA 
CTAGAGACAG AGCGGGAGCT CAAACAGAGG CTTCTGGAGG AGCAAGCCAA ATTGCAGCAG 
CAGATGGACC TGCAGAAGAA CCACATCTTC AGACTGACGC AAGGGCTGCA GGAGGCGCTG 
GACCGGGCGG ATCTGCTGAA GACAGAAAGG AGCGACCTGG AGTACCAGCT GGAAAACATT 
CAGGTTCTCT ACTCTCACGA GAAAGTGAAA ATGGAAGGCA CAATCTCTCA GCAAACCAAA 
CTCATTGATT TCCTGCAAGC CAAAATGGAC CAGCCTGCTA AAAAGAAAAA GGTTCCTCTG 
CAGTACAATG AGCTGAAGCT AGCCCTGGAA AAGGAGAAAG CCCGATGCGC GGAGCTGGAG 
GAGGCCCTTC AGAAGACCCG CATCGAACTC CGCTCTGCGC GGGAGGAAGC TGCCCACCGC 
AAAGCCACGG ACCACCCGCA CCCGTCTACG CCAGCCACTG CGAGGCAGCA GATCGCCATG 
TCTGCCATTG TGCGGTCGCC CGAGCACCAG CCCAGTGCCA TGAGCCTGCT TGCCCCACCA 
TCCAGCCGCA GAAAGGAGTC GTCAACTCCA GAGGAATTCA GCCGGCGTCT GAAAGAGCGC 
ATGCACCACA ACATCCCTCA CCGGTTTAAT GTGGGCCTGA ACATGAGAGC CACCAAGTGC 
GCCGTGTGTC TGGATACTGT GCACTTTGGA CGCCAGGCAT CCAAATGCCT CGAATGTCAA 
GTGATGTGTC ACCCCAAATG CTCCACCTGC TTGCCCGCTA CCTGTGGCCT GCCAGCTGAA 
TATGCCACAC ACTTCACTGA GGCCTTCTGC CGGGACAAAA TGAACTCCCC GGGGCTCCAG 
AGCAAGGAGC CTGGCAGCAG CTTGCACCTG GAAGGGTGGA TGAAAGTGCC CAGAAATAAC 
AAACGGGGAC AGCAAGGCTG GGACAGGAAG TACATTGTTC TGGAGGGGTC AAAAGTCCTC 
ATCTATGACA ATGAAGCCAG AGAAGCTGGA CAGAGGCCGG TGGAAGAATT TGAGCTGTGC 
CTTCCTGACG GGGACGTATC TATTCATGGT GCCGTTGGTG CTTCAGAACT TGCAAATACC 
GCCAAAGCAG ATGTCCCATA CATCCTGAAG ATGGAGTCTC ACCCACACAC CACCTGCTGG 
CCTGGGAGAA CCCTCTACTT GCTAGCACCC AGCTTCCCCG ACAAGCAGCG CTGGGTCACC 
GCCTTAGAAT CTGTCGTCGC AGGTGGGAGA GTTTCTAGGG AAAAGGCCGA AGCCGATGCT 
AAATTACTTG GAAACTCTCT GCTGAAACTG GAGGGCGATG ACCGGCTTGA CATGAACTGC 
ACCCTGCCCT TCAGTGACCA GGTAGTGCTG GTGGGCACGG AGGAAGGCCT CTACGCCCTG 
AATGTCTTGA AAAACTCCTT AACCCACATC CCAGGGATTG GCGCAGTCTT CCAAATTTAC 
ATCATCAAGG ACCTGGAGAA GCTGCTCATG ATAGCAGGGG AAGAGCGGGC TCTGTGCCTG 
GTGGACGTGA AGAAGGTGAA GCAGTCCCTG GCGCAGTCAC ACCTGCCTGC CCAGCCCGAC 
GTCTCCCCCA ACATATTCGA AGCCGTCAAA GGCTGCCACT TGTTCGCTGC TGGCAAGATC 
GAGAACAGCC TGTGCATCTG CGCCGCTATG CCAAGCAAAG TCGTCATCCT CCGCTACAAT 
GACAACCTCA GCAAGTACTG CATCCGCAAG GAGATCGAGA CCTCAGAGCC CTGCAGCTGT 
ATCCACTTCA CCAACTACAG CATCCTCATT GGGACCAACA AATTCTATGA GATCGACATG 
AAGCAGTACA CGCTTGACGA GTTCCTGGAC AAGAACGACC ATTCCTTGGC ACCTGCTGTG 
TTCGCCTCCT CGTCCAACAG CTTCCCTGTC TCCATTGTGC AGGCGAACAG CGCCGGGCAG 
CGAGAAGAAT ACCTGCTGTG CTTCCACGAA TTTGGGGTGT TCGTGGATTC TTACGGAAGA 
CGTAGCCGCA CAGATGATCT CAAGTGGAGT CGCTTACCTC TGGCCTTCGC CTACAGAGAA 
CCTTATCTGT TTGTGACTCA CTTCAACTCC CTGGAAGTCA TTGAGATCCA GGCCAGATCC 
TCACTGGGGA GCCCTGCCCG AGCATATCTG GAAATTCCAA ACCCTCGCTA CCTGGGCCCC 
GCGATTTCCT CCGGAGCGAT TTACCTGGCC TCCTCATACC AGGACAAGTT AAGGGTCATA 
TGCTGCAAAG GAAACCTCGT GAAGGAGTCA GGCACTGAGC AGCACCGGGT GCCCTCCACC 
TCCCGCAGCC CCAACAAGCG AGGCCCACCA ACATACAACG AGCACATCAC CAAACGCGTG 
GCCTCCAGCC CGGCGCCACC GGAAGGCCCC AGCCACCCCC GAGAGCCAAG CACACCGCAC 
CGCTACCGAG ACAGAGAGGG TCGGACAGAG CTGCGCAGGG ACAAGTCTCC AGGCCGCCCT 
CTGGAGCGGG AGAAGTCCCC AGGCCGAATG CTCAGCACTA GGAGAGAGCG GTCCCCAGGG 
AGACTGTTTG AAGACAGCAG CAGGGGCCGG CTGCCTGCAG GAGCAGTGAG GACCCCACTG 
TCCCAGGTTA ACAAGGTCTG GGACCAGTCT TCAGTATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011246465.1
CDS	157..6195
Translation	MLKFKYGVRNPPEASASEPIASRASRLNLFFQGKPPLMTQQQMSALSREGMLDALFALFEECSQPALMKMKHVSSFVQKYSDTIAELRELQPSARDFEVRSLVGCGHFAEVQVVREKATGDVYAMKIMKKKALLAQEQVSFFEEERNILSRSTSPWIPQLQYAFQDKNNLYLVMEYQPGGDLLSLLNRYEDQLDESMIQFYLAELILAVHSVHQMGYVHRDIKPENILIDRTGHIKLVDFGSAAKMNSNKVDAKLPIGTPDYMAPEVLTVMNEDRRGTYGLDCDWWSVGVVAYEMVYGKTPFTEGTSARTFNNIMNFQRFLKFPDDPKVSSELLDLLQSLLCVQKERLKFEGLCCHPFFARTDWNNIRNSPPPFVPTLKSDDDTSNFDEPEKNSWVSSSVCQLSPSGFSGEELPFVGFSYSKALGYLGRSESVVSSLDSPAKVSSMEKKLLIKSKELQDSQDKCHKMEQEMTRLHRRVSEVEAVLSQKEVELKASETQRSLLEQDLATYITECSSLKRSLEQARMEVSQEDDKALQLLHDIREQSRKLQEIKEQEYQAQVEEMRLMMNQLEEDLVSARRRSDLYESELRESRLAAEEFKRKANECQHKLMKAKDQGKPEVGEYSKLEKINAEQQLKIQELQEKLEKAVKASTEATELLQNIRQAKERAERELEKLHNREDSSEGIKKKLVEAEELEEKHREAQVSAQHLEVHLKQKEQHYEEKIKVLDNQIKKDLADKESLENMMQRHEEEAHEKGKILSEQKAMINAMDSKIRSLEQRIVELSEANKLAANSSLFTQRNMKAQEEMISELRQQKFYLETQAGKLEAQNRKLEEQLEKISHQDHSDKSRLLELETRLREVSLEHEEQKLELKRQLTELQLSLQERESQLTALQAARAALESQLRQAKTELEETTAEAEEEIQALTAHRDEIQRKFDALRNSCTVITDLEEQLNQLTEDNAELNNQNFYLSKQLDEASGANDEIVQLRSEVDHLRREITEREMQLTSQKQTMEALKTTCTMLEEQVLDLEALNDELLEKERQWEAWRSVLGDEKSQFECRVRELQRMLDTEKQSRARADQRITESRQVVELAVKEHKAEILALQQALKEQKLKAESLSDKLNDLEKKHAMLEMNARSLQQKLETERELKQRLLEEQAKLQQQMDLQKNHIFRLTQGLQEALDRADLLKTERSDLEYQLENIQVLYSHEKVKMEGTISQQTKLIDFLQAKMDQPAKKKKVPLQYNELKLALEKEKARCAELEEALQKTRIELRSAREEAAHRKATDHPHPSTPATARQQIAMSAIVRSPEHQPSAMSLLAPPSSRRKESSTPEEFSRRLKERMHHNIPHRFNVGLNMRATKCAVCLDTVHFGRQASKCLECQVMCHPKCSTCLPATCGLPAEYATHFTEAFCRDKMNSPGLQSKEPGSSLHLEGWMKVPRNNKRGQQGWDRKYIVLEGSKVLIYDNEAREAGQRPVEEFELCLPDGDVSIHGAVGASELANTAKADVPYILKMESHPHTTCWPGRTLYLLAPSFPDKQRWVTALESVVAGGRVSREKAEADAKLLGNSLLKLEGDDRLDMNCTLPFSDQVVLVGTEEGLYALNVLKNSLTHIPGIGAVFQIYIIKDLEKLLMIAGEERALCLVDVKKVKQSLAQSHLPAQPDVSPNIFEAVKGCHLFAAGKIENSLCICAAMPSKVVILRYNDNLSKYCIRKEIETSEPCSCIHFTNYSILIGTNKFYEIDMKQYTLDEFLDKNDHSLAPAVFASSSNSFPVSIVQANSAGQREEYLLCFHEFGVFVDSYGRRSRTDDLKWSRLPLAFAYREPYLFVTHFNSLEVIEIQARSSLGSPARAYLEIPNPRYLGPAISSGAIYLASSYQDKLRVICCKGNLVKESGTEQHRVPSTSRSPNKRGPPTYNEHITKRVASSPAPPEGPSHPREPSTPHRYRDREGRTELRRDKSPGRPLEREKSPGRMLSTRRERSPGRLFEDSSRGRLPAGAVRTPLSQVNKVWDQSSV

Target ORF information:

RefSeq Version	XM_011248163.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus citron (Cit), transcript variant X20, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011248163.3

ORF Insert Sequence:

ATGTTGAAGT TCAAGTATGG TGTGCGGAAC CCGCCGGAGG CCAGTGCCTC CGAGCCCATT 
GCCAGTCGGG CCTCCAGGCT AAATCTCTTC TTCCAGGGGA AACCGCCCCT CATGACTCAA 
CAGCAGATGT CTGCTCTTTC CCGGGAAGGG ATGCTAGACG CCCTCTTCGC TCTCTTTGAA 
GAGTGCAGCC AACCCGCCCT GATGAAGATG AAGCACGTGA GCAGCTTTGT CCAGAAGTAT 
TCCGACACCA TAGCCGAGTT GCGGGAGCTG CAGCCGTCGG CGAGAGACTT CGAAGTTCGA 
AGCCTTGTGG GCTGTGGTCA CTTCGCTGAA GTGCAGGTGG TTAGAGAGAA GGCGACCGGG 
GACGTCTATG CCATGAAAAT CATGAAGAAG AAGGCTTTGC TGGCCCAGGA ACAGGTTTCA 
TTTTTCGAGG AGGAGAGGAA CATATTATCT CGGAGCACGA GTCCTTGGAT CCCCCAGTTA 
CAGTACGCCT TTCAGGACAA AAATAACCTT TACCTGGTCA TGGAATATCA GCCTGGAGGG 
GATTTGCTGT CGCTTCTGAA CAGATACGAG GACCAATTAG ATGAGAGCAT GATCCAGTTT 
TACCTTGCTG AGCTGATTTT GGCTGTCCAC AGCGTGCACC AGATGGGATA TGTGCATCGA 
GACATCAAGC CCGAGAACAT CCTCATCGAC CGGACGGGAC ACATCAAGCT GGTGGATTTT 
GGATCAGCCG CTAAGATGAA TTCAAATAAG GTGGATGCCA AACTCCCCAT TGGGACCCCG 
GATTACATGG CTCCGGAAGT GTTGACCGTG ATGAACGAGG ACCGAAGGGG CACATACGGC 
TTGGACTGTG ACTGGTGGTC TGTCGGAGTT GTTGCTTATG AGATGGTTTA TGGGAAGACC 
CCATTCACAG AGGGAACCTC CGCCCGGACC TTCAACAACA TCATGAACTT CCAGCGGTTT 
TTGAAGTTCC CAGATGACCC CAAAGTTAGC AGTGAGCTCC TTGATCTGCT TCAGAGTCTG 
CTGTGTGTCC AGAAAGAGAG ACTGAAGTTC GAGGGTCTCT GCTGCCACCC TTTCTTTGCC 
AGAACGGACT GGAACAACAT CCGTAACTCT CCTCCCCCCT TCGTCCCCAC CCTCAAGTCT 
GACGATGACA CCTCCAATTT TGATGAACCA GAGAAGAATT CGTGGGTTTC ATCCTCTGTG 
TGCCAGCTGA GCCCCTCGGG CTTCTCAGGC GAAGAGCTGC CGTTTGTGGG ATTTTCGTAC 
AGCAAGGCAC TGGGGTATCT TGGTAGATCT GAGTCTGTCG TGTCGAGTCT GGACTCCCCT 
GCCAAGGTTA GCTCCATGGA AAAGAAACTT CTCATCAAAA GCAAAGAGCT CCAAGACTCC 
CAGGACAAGT GTCACAAGAT GGAGCAGGAA ATGACCCGGT TACATCGCAG AGTGTCAGAG 
GTGGAGGCTG TGCTTAGTCA GAAGGAGGTG GAGCTGAAGG CCTCTGAGAC TCAGAGATCC 
CTCCTGGAGC AGGACCTTGC TACCTACATC ACAGAATGCA GTAGCTTAAA GCGAAGTTTG 
GAGCAAGCGC GGATGGAGGT GTCCCAGGAG GATGACAAAG CTCTGCAGCT TCTCCACGAC 
ATCCGAGAGC AGAGCCGGAA GCTCCAGGAG ATCAAGGAGC AGGAGTACCA GGCTCAGGTG 
GAGGAGATGA GGCTGATGAT GAATCAGCTG GAAGAAGACC TGGTGTCAGC CCGCAGACGC 
AGCGATCTCT ACGAGTCTGA GCTGAGGGAG TCTCGGCTTG CCGCCGAGGA ATTCAAGCGG 
AAGGCAAACG AATGTCAGCA CAAACTGATG AAGGCTAAGG ACCAAGGGAA GCCTGAAGTG 
GGAGAATATT CCAAACTGGA GAAGATCAAT GCTGAGCAGC AGCTGAAGAT CCAGGAGCTC 
CAGGAGAAGC TGGAGAAGGC TGTAAAAGCC AGCACAGAGG CCACCGAGCT CCTGCAGAAT 
ATCCGCCAGG CAAAGGAGCG AGCAGAGCGG GAGCTAGAGA AGCTACACAA CCGGGAAGAC 
TCCTCCGAGG GCATCAAAAA GAAGCTGGTG GAAGCCGAGG AGCTGGAGGA GAAACATCGG 
GAGGCTCAGG TCTCAGCTCA ACATCTAGAA GTACACTTGA AACAGAAAGA ACAGCACTAC 
GAGGAAAAGA TCAAGGTATT GGACAATCAG ATAAAGAAGG ACCTGGCCGA CAAGGAGAGC 
CTGGAGAACA TGATGCAGAG ACACGAGGAG GAGGCCCACG AGAAGGGCAA GATCCTCAGC 
GAGCAGAAGG CGATGATCAA CGCGATGGAT TCCAAGATCC GATCCCTGGA GCAGAGGATC 
GTGGAGCTGT CGGAAGCCAA CAAGCTTGCG GCAAACAGCA GTCTCTTCAC CCAGAGGAAC 
ATGAAGGCCC AGGAAGAGAT GATCTCAGAA CTCAGGCAGC AGAAATTTTA CCTGGAGACG 
CAGGCCGGAA AGCTGGAGGC CCAGAACCGA AAGCTGGAAG AGCAACTGGA GAAGATCAGC 
CACCAAGATC ACAGTGACAA GAGTCGGCTG CTGGAGCTGG AAACAAGGCT GAGGGAGGTC 
AGCCTGGAGC ACGAGGAGCA GAAGCTGGAG CTGAAGCGGC AGCTCACGGA GCTGCAGCTG 
TCCCTGCAGG AGCGCGAGTC CCAGCTGACG GCCCTGCAGG CCGCCCGGGC AGCGCTGGAG 
AGCCAGCTCC GCCAGGCGAA GACAGAGCTG GAGGAGACAA CCGCGGAAGC GGAGGAGGAG 
ATCCAGGCGC TCACGGCACA TCGAGATGAG ATCCAGCGCA AATTCGATGC CCTTCGCAAC 
AGCTGCACGG TCATCACCGA CCTGGAGGAG CAGCTGAACC AGCTCACCGA GGACAACGCC 
GAGCTCAACA ACCAAAACTT CTACCTGTCC AAACAACTCG ATGAGGCTTC CGGGGCCAAT 
GACGAGATTG TGCAGCTGCG GAGTGAGGTG GACCATCTCC GCCGTGAGAT CACGGAGCGG 
GAGATGCAGC TCACCAGCCA GAAGCAAACG ATGGAGGCTC TGAAGACGAC ATGCACCATG 
CTGGAGGAGC AGGTCCTGGA CCTGGAGGCC CTGAACGACG AGCTGCTGGA GAAGGAGCGC 
CAGTGGGAGG CCTGGCGGAG CGTCCTCGGC GACGAGAAGT CCCAGTTCGA GTGTCGAGTT 
CGAGAGCTAC AGAGGATGCT GGACACCGAG AAGCAGAGCA GGGCTAGGGC CGATCAGCGG 
ATCACCGAGT CGCGCCAGGT GGTGGAGTTG GCGGTGAAGG AACACAAGGC CGAGATTCTT 
GCTCTGCAGC AGGCTCTCAA GGAGCAGAAG CTCAAAGCCG AGAGCCTGTC GGACAAGCTC 
AACGACCTGG AGAAGAAGCA CGCCATGCTG GAGATGAACG CCCGGAGCTT ACAGCAGAAA 
CTAGAGACAG AGCGGGAGCT CAAACAGAGG CTTCTGGAGG AGCAAGCCAA ATTGCAGCAG 
CAGATGGACC TGCAGAAGAA CCACATCTTC AGACTGACGC AAGGGCTGCA GGAGGCGCTG 
GACCGGGCGG ATCTGCTGAA GACAGAAAGG AGCGACCTGG AGTACCAGCT GGAAAACATT 
CAGGTTCTCT ACTCTCACGA GAAAGTGAAA ATGGAAGGCA CAATCTCTCA GCAAACCAAA 
CTCATTGATT TCCTGCAAGC CAAAATGGAC CAGCCTGCTA AAAAGAAAAA GGTTCCTCTG 
CAGTACAATG AGCTGAAGCT AGCCCTGGAA AAGGAGAAAG CCCGATGCGC GGAGCTGGAG 
GAGGCCCTTC AGAAGACCCG CATCGAACTC CGCTCTGCGC GGGAGGAAGC TGCCCACCGC 
AAAGCCACGG ACCACCCGCA CCCGTCTACG CCAGCCACTG CGAGGCAGCA GATCGCCATG 
TCTGCCATTG TGCGGTCGCC CGAGCACCAG CCCAGTGCCA TGAGCCTGCT TGCCCCACCA 
TCCAGCCGCA GAAAGGAGTC GTCAACTCCA GAGGAATTCA GCCGGCGTCT GAAAGAGCGC 
ATGCACCACA ACATCCCTCA CCGGTTTAAT GTGGGCCTGA ACATGAGAGC CACCAAGTGC 
GCCGTGTGTC TGGATACTGT GCACTTTGGA CGCCAGGCAT CCAAATGCCT CGAATGTCAA 
GTGATGTGTC ACCCCAAATG CTCCACCTGC TTGCCCGCTA CCTGTGGCCT GCCAGCTGAA 
TATGCCACAC ACTTCACTGA GGCCTTCTGC CGGGACAAAA TGAACTCCCC GGGGCTCCAG 
AGCAAGGAGC CTGGCAGCAG CTTGCACCTG GAAGGGTGGA TGAAAGTGCC CAGAAATAAC 
AAACGGGGAC AGCAAGGCTG GGACAGGAAG TACATTGTTC TGGAGGGGTC AAAAGTCCTC 
ATCTATGACA ATGAAGCCAG AGAAGCTGGA CAGAGGCCGG TGGAAGAATT TGAGCTGTGC 
CTTCCTGACG GGGACGTATC TATTCATGGT GCCGTTGGTG CTTCAGAACT TGCAAATACC 
GCCAAAGCAG ATGTCCCATA CATCCTGAAG ATGGAGTCTC ACCCACACAC CACCTGCTGG 
CCTGGGAGAA CCCTCTACTT GCTAGCACCC AGCTTCCCCG ACAAGCAGCG CTGGGTCACC 
GCCTTAGAAT CTGTCGTCGC AGGTGGGAGA GTTTCTAGGG AAAAGGCCGA AGCCGATGCT 
AAATTACTTG GAAACTCTCT GCTGAAACTG GAGGGCGATG ACCGGCTTGA CATGAACTGC 
ACCCTGCCCT TCAGTGACCA GGTAGTGCTG GTGGGCACGG AGGAAGGCCT CTACGCCCTG 
AATGTCTTGA AAAACTCCTT AACCCACATC CCAGGGATTG GCGCAGTCTT CCAAATTTAC 
ATCATCAAGG ACCTGGAGAA GCTGCTCATG ATAGCAGGGG AAGAGCGGGC TCTGTGCCTG 
GTGGACGTGA AGAAGGTGAA GCAGTCCCTG GCGCAGTCAC ACCTGCCTGC CCAGCCCGAC 
GTCTCCCCCA ACATATTCGA AGCCGTCAAA GGCTGCCACT TGTTCGCTGC TGGCAAGATC 
GAGAACAGCC TGTGCATCTG CGCCGCTATG CCAAGCAAAG TCGTCATCCT CCGCTACAAT 
GACAACCTCA GCAAGTACTG CATCCGCAAG GAGATCGAGA CCTCAGAGCC CTGCAGCTGT 
ATCCACTTCA CCAACTACAG CATCCTCATT GGGACCAACA AATTCTATGA GATCGACATG 
AAGCAGTACA CGCTTGACGA GTTCCTGGAC AAGAACGACC ATTCCTTGGC ACCTGCTGTG 
TTCGCCTCCT CGTCCAACAG CTTCCCTGTC TCCATTGTGC AGGCGAACAG CGCCGGGCAG 
CGAGAAGAAT ACCTGCTGTG CTTCCACGAA TTTGGGGTGT TCGTGGATTC TTACGGAAGA 
CGTAGCCGCA CAGATGATCT CAAGTGGAGT CGCTTACCTC TGGCCTTCGC CTACAGAGAA 
CCTTATCTGT TTGTGACTCA CTTCAACTCC CTGGAAGTCA TTGAGATCCA GGCCAGATCC 
TCACTGGGGA GCCCTGCCCG AGCATATCTG GAAATTCCAA ACCCTCGCTA CCTGGGCCCC 
GCGATTTCCT CCGGAGCGAT TTACCTGGCC TCCTCATACC AGGACAAGTT AAGGGTCATA 
TGCTGCAAAG GAAACCTCGT GAAGGAGTCA GGCACTGAGC AGCACCGGGT GCCCTCCACC 
TCCCGCAGCC CCAACAAGCG AGGCCCACCA ACATACAACG AGCACATCAC CAAACGCGTG 
GCCTCCAGCC CGGCGCCACC GGAAGGCCCC AGCCACCCCC GAGAGCCAAG CACACCGCAC 
CGCTACCGAG ACAGAGAGGG TCGGACAGAG CTGCGCAGGG ACAAGTCTCC AGGCCGCCCT 
CTGGAGCGGG AGAAGTCCCC AGGCCGAATG CTCAGCACTA GGAGAGAGCG GTCCCCAGGG 
AGACTGTTTG AAGACAGCAG CAGGGGCCGG CTGCCTGCAG GAGCAGTGAG GACCCCACTG 
TCCCAGGTTA ACAAGGTCTG GGACCAGTCT TCAGTATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cit ( XM_017320640.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017176129.1 Mus musculus citron (Cit), transcript variant X18, mRNA.

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CloneID	OMu81231
Clone ID Related Accession (Same CDS sequence)	XM_017320640.1
Accession Version	XM_017320640.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6054bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	citron Rho-interacting kinase isoform X14
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000071.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTGAAGT TCAAGTATGG TGTGCGGAAC CCGCCGGAGG CCAGTGCCTC CGAGCCCATT 
GCCAGTCGGG CCTCCAGGCT AAATCTCTTC TTCCAGGGGA AACCGCCCCT CATGACTCAA 
CAGCAGATGT CTGCTCTTTC CCGGGAAGGG ATGCTAGACG CCCTCTTCGC TCTCTTTGAA 
GAGTGCAGCC AACCCGCCCT GATGAAGATG AAGCACGTGA GCAGCTTTGT CCAGAAGTAT 
TCCGACACCA TAGCCGAGTT GCGGGAGCTG CAGCCGTCGG CGAGAGACTT CGAAGTTCGA 
AGCCTTGTGG GCTGTGGTCA CTTCGCTGAA GTGCAGGTGG TTAGAGAGAA GGCGACCGGG 
GACGTCTATG CCATGAAAAT CATGAAGAAG AAGGCTTTGC TGGCCCAGGA ACAGGTTTCA 
TTTTTCGAGG AGGAGAGGAA CATATTATCT CGGAGCACGA GTCCTTGGAT CCCCCAGTTA 
CAGTACGCCT TTCAGGACAA AAATAACCTT TACCTGGTCA TGGAATATCA GCCTGGAGGG 
GATTTGCTGT CGCTTCTGAA CAGATACGAG GACCAATTAG ATGAGAGCAT GATCCAGTTT 
TACCTTGCTG AGCTGATTTT GGCTGTCCAC AGCGTGCACC AGATGGGATA TGTGCATCGA 
GACATCAAGC CCGAGAACAT CCTCATCGAC CGGACGGGAC ACATCAAGCT GGTGGATTTT 
GGATCAGCCG CTAAGATGAA TTCAAATAAG GTGGATGCCA AACTCCCCAT TGGGACCCCG 
GATTACATGG CTCCGGAAGT GTTGACCGTG ATGAACGAGG ACCGAAGGGG CACATACGGC 
TTGGACTGTG ACTGGTGGTC TGTCGGAGTT GTTGCTTATG AGATGGTTTA TGGGAAGACC 
CCATTCACAG AGGGAACCTC CGCCCGGACC TTCAACAACA TCATGAACTT CCAGCGGTTT 
TTGAAGTTCC CAGATGACCC CAAAGTTAGC AGTGAGCTCC TTGATCTGCT TCAGAGTCTG 
CTGTGTGTCC AGAAAGAGAG ACTGAAGTTC GAGGGTCTCT GCTGCCACCC TTTCTTTGCC 
AGAACGGACT GGAACAACAT CCGTAACTCT CCTCCCCCCT TCGTCCCCAC CCTCAAGTCT 
GACGATGACA CCTCCAATTT TGATGAACCA GAGAAGAATT CGTGGGTTTC ATCCTCTGTG 
TGCCAGCTGA GCCCCTCGGG CTTCTCAGGC GAAGAGCTGC CGTTTGTGGG ATTTTCGTAC 
AGCAAGGCAC TGGGGTATCT TGGTAGATCT GAGTCTGTCG TGTCGAGTCT GGACTCCCCT 
GCCAAGGTTA GCTCCATGGA AAAGAAACTT CTCATCAAAA GCAAAGAGCT CCAAGACTCC 
CAGGACAAGT GTCACAAGAT GGAGCAGGAA ATGACCCGGT TACATCGCAG AGTGTCAGAG 
GTGGAGGCTG TGCTTAGTCA GAAGGAGGTG GAGCTGAAGG CCTCTGAGAC TCAGAGATCC 
CTCCTGGAGC AGGACCTTGC TACCTACATC ACAGAATGCA GTAGCTTAAA GCGAAGTTTG 
GAGCAAGCGC GGATGGAGGT GTCCCAGGAG GATGACAAAG CTCTGCAGCT TCTCCACGAC 
ATCCGAGAGC AGAGCCGGAA GCTCCAGGAG ATCAAGGAGC AGGAGTACCA GGCTCAGGTG 
GAGGAGATGA GGCTGATGAT GAATCAGCTG GAAGAAGACC TGGTGTCAGC CCGCAGACGC 
AGCGATCTCT ACGAGTCTGA GCTGAGGGAG TCTCGGCTTG CCGCCGAGGA ATTCAAGCGG 
AAGGCAAACG AATGTCAGCA CAAACTGATG AAGGTAGTCA GCCACCCTCC AAGAGGGGAC 
TCCGGGGGCA CGGCCCTGGA TGATCTTCAC AAGATGCAAG GCCACGCTGG CTTAACGAGT 
GCTAAGGACC AAGGGAAGCC TGAAGTGGGA GAATATTCCA AACTGGAGAA GATCAATGCT 
GAGCAGCAGC TGAAGATCCA GGAGCTCCAG GAGAAGCTGG AGAAGGCTGT AAAAGCCAGC 
ACAGAGGCCA CCGAGCTCCT GCAGAATATC CGCCAGGCAA AGGAGCGAGC AGAGCGGGAG 
CTAGAGAAGC TACACAACCG GGAAGACTCC TCCGAGGGCA TCAAAAAGAA GCTGGTGGAA 
GCCGAGGAAC GCCGCCACTC CCTGGAGAAC AAGGTAAAGA GACTAGAGAC CATGGAGCGT 
AGAGAGAACA GACTGAAGGA TGACATCCAG ACAAAGTCCG AACAGATCCA GCAGATGGCT 
GATAAAATTC TGGAGCTGGA GGAGAAACAT CGGGAGGCTC AGGTCTCAGC TCAACATCTA 
GAAGTACACT TGAAACAGAA AGAACAGCAC TACGAGGAAA AGATCAAGGT ATTGGACAAT 
CAGATAAAGA AGGACCTGGC CGACAAGGAG AGCCTGGAGA ACATGATGCA GAGACACGAG 
GAGGAGGCCC ACGAGAAGGG CAAGATCCTC AGCGAGCAGA AGGCGATGAT CAACGCGATG 
GATTCCAAGA TCCGATCCCT GGAGCAGAGG ATCGTGGAGC TGTCGGAAGC CAACAAGCTT 
GCGGCAAACA GCAGTCTCTT CACCCAGAGG AACATGAAGG CCCAGGAAGA GATGATCTCA 
GAACTCAGGC AGCAGAAATT TTACCTGGAG ACGCAGGCCG GAAAGCTGGA GGCCCAGAAC 
CGAAAGCTGG AAGAGCAACT GGAGAAGATC AGCCACCAAG ATCACAGTGA CAAGAGTCGG 
CTGCTGGAGC TGGAAACAAG GCTGAGGGAG GTCAGCCTGG AGCACGAGGA GCAGAAGCTG 
GAGCTGAAGC GGCAGCTCAC GGAGCTGCAG CTGTCCCTGC AGGAGCGCGA GTCCCAGCTG 
ACGGCCCTGC AGGCCGCCCG GGCAGCGCTG GAGAGCCAGC TCCGCCAGGC GAAGACAGAG 
CTGGAGGAGA CAACCGCGGA AGCGGAGGAG GAGATCCAGG CGCTCACGGC ACATCGAGAT 
GAGATCCAGC GCAAATTCGA TGCCCTTCGC AACAGCTGCA CGGTCATCAC CGACCTGGAG 
GAGCAGCTGA ACCAGCTCAC CGAGGACAAC GCCGAGCTCA ACAACCAAAA CTTCTACCTG 
TCCAAACAAC TCGATGAGGC TTCCGGGGCC AATGACGAGA TTGTGCAGCT GCGGAGTGAG 
GTGGACCATC TCCGCCGTGA GATCACGGAG CGGGAGATGC AGCTCACCAG CCAGAAGCAA 
GCACAACTCA GTGCTCCTGA TTTGCAGACG ATGGAGGCTC TGAAGACGAC ATGCACCATG 
CTGGAGGAGC AGGTCCTGGA CCTGGAGGCC CTGAACGACG AGCTGCTGGA GAAGGAGCGC 
CAGTGGGAGG CCTGGCGGAG CGTCCTCGGC GACGAGAAGT CCCAGTTCGA GTGTCGAGTT 
CGAGAGCTAC AGAGGATGCT GGACACCGAG AAGCAGAGCA GGGCTAGGGC CGATCAGCGG 
ATCACCGAGT CGCGCCAGGT GGTGGAGTTG GCGGTGAAGG AACACAAGGC CGAGATTCTT 
GCTCTGCAGC AGGCTCTCAA GGAGCAGAAG CTCAAAGCCG AGAGCCTGTC GGACAAGCTC 
AACGACCTGG AGAAGAAGCA CGCCATGCTG GAGATGAACG CCCGGAGCTT ACAGCAGAAA 
CTAGAGACAG AGCGGGAGCT CAAACAGAGG CTTCTGGAGG AGCAAGCCAA ATTGCAGCAG 
CAGATGGACC TGCAGAAGAA CCACATCTTC AGACTGACGC AAGGGCTGCA GGAGGCGCTG 
GACCGGGCGG ATCTGCTGAA GACAGAAAGG AGCGACCTGG AGTACCAGCT GGAAAACATT 
CAGGTTCTCT ACTCTCACGA GAAAGTGAAA ATGGAAGGCA CAATCTCTCA GCAAACCAAA 
CTCATTGATT TCCTGCAAGC CAAAATGGAC CAGCCTGCTA AAAAGAAAAA GGGTTTATTT 
AGTCGACGGA AAGAGGACCC TGCTTTGCCC ACACAGGTTC CTCTGCAGTA CAATGAGCTG 
AAGCTAGCCC TGGAAAAGGA GAAAGCCCGA TGCGCGGAGC TGGAGGAGGC CCTTCAGAAG 
ACCCGCATCG AACTCCGCTC TGCGCGGGAG GAAGCTGCCC ACCGCAAAGC CACGGACCAC 
CCGCACCCGT CTACGCCAGC CACTGCGAGG CAGCAGATCG CCATGTCTGC CATTGTGCGG 
TCGCCCGAGC ACCAGCCCAG TGCCATGAGC CTGCTTGCCC CACCATCCAG CCGCAGAAAG 
GAGTCGTCAA CTCCAGAGGA ATTCAGCCGG CGTCTGAAAG AGCGCATGCA CCACAACATC 
CCTCACCGGT TTAATGTGGG CCTGAACATG AGAGCCACCA AGTGCGCCGT GTGTCTGGAT 
ACTGTGCACT TTGGACGCCA GGCATCCAAA TGCCTCGAAT GTCAAGTGAT GTGTCACCCC 
AAATGCTCCA CCTGCTTGCC CGCTACCTGT GGCCTGCCAG CTGAATATGC CACACACTTC 
ACTGAGGCCT TCTGCCGGGA CAAAATGAAC TCCCCGGGGC TCCAGAGCAA GGAGCCTGGC 
AGCAGCTTGC ACCTGGAAGG GTGGATGAAA GTGCCCAGAA ATAACAAACG GGGACAGCAA 
GGCTGGGACA GGAAGTACAT TGTTCTGGAG GGGTCAAAAG TCCTCATCTA TGACAATGAA 
GCCAGAGAAG CTGGACAGAG GCCGGTGGAA GAATTTGAGC TGTGCCTTCC TGACGGGGAC 
GTATCTATTC ATGGTGCCGT TGGTGCTTCA GAACTTGCAA ATACCGCCAA AGCAGATGTC 
CCATACATCC TGAAGATGGA GTCTCACCCA CACACCACCT GCTGGCCTGG GAGAACCCTC 
TACTTGCTAG CACCCAGCTT CCCCGACAAG CAGCGCTGGG TCACCGCCTT AGAATCTGTC 
GTCGCAGGTG GGAGAGTTTC TAGGGAAAAG GCCGAAGCCG ATGCTGCACG TGACCATACT 
TCGAGTGAGC ATCAGCCAGT GTGGGTTGAG AAATTACTTG GAAACTCTCT GCTGAAACTG 
GAGGGCGATG ACCGGCTTGA CATGAACTGC ACCCTGCCCT TCAGTGACCA GGTAGTGCTG 
GTGGGCACGG AGGAAGGCCT CTACGCCCTG AATGTCTTGA AAAACTCCTT AACCCACATC 
CCAGGGATTG GCGCAGTCTT CCAAATTTAC ATCATCAAGG ACCTGGAGAA GCTGCTCATG 
ATAGCAGGGG AAGAGCGGGC TCTGTGCCTG GTGGACGTGA AGAAGGTGAA GCAGTCCCTG 
GCGCAGTCAC ACCTGCCTGC CCAGCCCGAC GTCTCCCCCA ACATATTCGA AGCCGTCAAA 
GGCTGCCACT TGTTCGCTGC TGGCAAGATC GAGAACAGCC TGTGCATCTG CGCCGCTATG 
CCAAGCAAAG TCGTCATCCT CCGCTACAAT GACAACCTCA GCAAGTACTG CATCCGCAAG 
GAGATCGAGA CCTCAGAGCC CTGCAGCTGT ATCCACTTCA CCAACTACAG CATCCTCATT 
GGGACCAACA AATTCTATGA GATCGACATG AAGCAGTACA CGCTTGACGA GTTCCTGGAC 
AAGAACGACC ATTCCTTGGC ACCTGCTGTG TTCGCCTCCT CGTCCAACAG CTTCCCTGTC 
TCCATTGTGC AGGCGAACAG CGCCGGGCAG CGAGAAGAAT ACCTGCTGTG CTTCCACGAA 
TTTGGGGTGT TCGTGGATTC TTACGGAAGA CGTAGCCGCA CAGATGATCT CAAGTGGAGT 
CGCTTACCTC TGGCCTTCGC CTACAGAGAA CCTTATCTGT TTGTGACTCA CTTCAACTCC 
CTGGAAGTCA TTGAGATCCA GGCCAGATCC TCACTGGGGA GCCCTGCCCG AGCATATCTG 
GAAATTCCAA ACCCTCGCTA CCTGGGCCCC GCGATTTCCT CCGGAGCGAT TTACCTGGCC 
TCCTCATACC AGGACAAGTT AAGGGTCATA TGCTGCAAAG GAAACCTCGT GAAGGAGTCA 
GGCACTGAGC AGCACCGGGT GCCCTCCACC TCCCGCAGCT CCTTCACTGC CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017176129.1
CDS	158..6211
Translation	MLKFKYGVRNPPEASASEPIASRASRLNLFFQGKPPLMTQQQMSALSREGMLDALFALFEECSQPALMKMKHVSSFVQKYSDTIAELRELQPSARDFEVRSLVGCGHFAEVQVVREKATGDVYAMKIMKKKALLAQEQVSFFEEERNILSRSTSPWIPQLQYAFQDKNNLYLVMEYQPGGDLLSLLNRYEDQLDESMIQFYLAELILAVHSVHQMGYVHRDIKPENILIDRTGHIKLVDFGSAAKMNSNKVDAKLPIGTPDYMAPEVLTVMNEDRRGTYGLDCDWWSVGVVAYEMVYGKTPFTEGTSARTFNNIMNFQRFLKFPDDPKVSSELLDLLQSLLCVQKERLKFEGLCCHPFFARTDWNNIRNSPPPFVPTLKSDDDTSNFDEPEKNSWVSSSVCQLSPSGFSGEELPFVGFSYSKALGYLGRSESVVSSLDSPAKVSSMEKKLLIKSKELQDSQDKCHKMEQEMTRLHRRVSEVEAVLSQKEVELKASETQRSLLEQDLATYITECSSLKRSLEQARMEVSQEDDKALQLLHDIREQSRKLQEIKEQEYQAQVEEMRLMMNQLEEDLVSARRRSDLYESELRESRLAAEEFKRKANECQHKLMKVVSHPPRGDSGGTALDDLHKMQGHAGLTSAKDQGKPEVGEYSKLEKINAEQQLKIQELQEKLEKAVKASTEATELLQNIRQAKERAERELEKLHNREDSSEGIKKKLVEAEERRHSLENKVKRLETMERRENRLKDDIQTKSEQIQQMADKILELEEKHREAQVSAQHLEVHLKQKEQHYEEKIKVLDNQIKKDLADKESLENMMQRHEEEAHEKGKILSEQKAMINAMDSKIRSLEQRIVELSEANKLAANSSLFTQRNMKAQEEMISELRQQKFYLETQAGKLEAQNRKLEEQLEKISHQDHSDKSRLLELETRLREVSLEHEEQKLELKRQLTELQLSLQERESQLTALQAARAALESQLRQAKTELEETTAEAEEEIQALTAHRDEIQRKFDALRNSCTVITDLEEQLNQLTEDNAELNNQNFYLSKQLDEASGANDEIVQLRSEVDHLRREITEREMQLTSQKQAQLSAPDLQTMEALKTTCTMLEEQVLDLEALNDELLEKERQWEAWRSVLGDEKSQFECRVRELQRMLDTEKQSRARADQRITESRQVVELAVKEHKAEILALQQALKEQKLKAESLSDKLNDLEKKHAMLEMNARSLQQKLETERELKQRLLEEQAKLQQQMDLQKNHIFRLTQGLQEALDRADLLKTERSDLEYQLENIQVLYSHEKVKMEGTISQQTKLIDFLQAKMDQPAKKKKGLFSRRKEDPALPTQVPLQYNELKLALEKEKARCAELEEALQKTRIELRSAREEAAHRKATDHPHPSTPATARQQIAMSAIVRSPEHQPSAMSLLAPPSSRRKESSTPEEFSRRLKERMHHNIPHRFNVGLNMRATKCAVCLDTVHFGRQASKCLECQVMCHPKCSTCLPATCGLPAEYATHFTEAFCRDKMNSPGLQSKEPGSSLHLEGWMKVPRNNKRGQQGWDRKYIVLEGSKVLIYDNEAREAGQRPVEEFELCLPDGDVSIHGAVGASELANTAKADVPYILKMESHPHTTCWPGRTLYLLAPSFPDKQRWVTALESVVAGGRVSREKAEADAARDHTSSEHQPVWVEKLLGNSLLKLEGDDRLDMNCTLPFSDQVVLVGTEEGLYALNVLKNSLTHIPGIGAVFQIYIIKDLEKLLMIAGEERALCLVDVKKVKQSLAQSHLPAQPDVSPNIFEAVKGCHLFAAGKIENSLCICAAMPSKVVILRYNDNLSKYCIRKEIETSEPCSCIHFTNYSILIGTNKFYEIDMKQYTLDEFLDKNDHSLAPAVFASSSNSFPVSIVQANSAGQREEYLLCFHEFGVFVDSYGRRSRTDDLKWSRLPLAFAYREPYLFVTHFNSLEVIEIQARSSLGSPARAYLEIPNPRYLGPAISSGAIYLASSYQDKLRVICCKGNLVKESGTEQHRVPSTSRSSFTA

Target ORF information:

RefSeq Version	XM_017320640.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus citron (Cit), transcript variant X18, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017320640.1

ORF Insert Sequence:

ATGTTGAAGT TCAAGTATGG TGTGCGGAAC CCGCCGGAGG CCAGTGCCTC CGAGCCCATT 
GCCAGTCGGG CCTCCAGGCT AAATCTCTTC TTCCAGGGGA AACCGCCCCT CATGACTCAA 
CAGCAGATGT CTGCTCTTTC CCGGGAAGGG ATGCTAGACG CCCTCTTCGC TCTCTTTGAA 
GAGTGCAGCC AACCCGCCCT GATGAAGATG AAGCACGTGA GCAGCTTTGT CCAGAAGTAT 
TCCGACACCA TAGCCGAGTT GCGGGAGCTG CAGCCGTCGG CGAGAGACTT CGAAGTTCGA 
AGCCTTGTGG GCTGTGGTCA CTTCGCTGAA GTGCAGGTGG TTAGAGAGAA GGCGACCGGG 
GACGTCTATG CCATGAAAAT CATGAAGAAG AAGGCTTTGC TGGCCCAGGA ACAGGTTTCA 
TTTTTCGAGG AGGAGAGGAA CATATTATCT CGGAGCACGA GTCCTTGGAT CCCCCAGTTA 
CAGTACGCCT TTCAGGACAA AAATAACCTT TACCTGGTCA TGGAATATCA GCCTGGAGGG 
GATTTGCTGT CGCTTCTGAA CAGATACGAG GACCAATTAG ATGAGAGCAT GATCCAGTTT 
TACCTTGCTG AGCTGATTTT GGCTGTCCAC AGCGTGCACC AGATGGGATA TGTGCATCGA 
GACATCAAGC CCGAGAACAT CCTCATCGAC CGGACGGGAC ACATCAAGCT GGTGGATTTT 
GGATCAGCCG CTAAGATGAA TTCAAATAAG GTGGATGCCA AACTCCCCAT TGGGACCCCG 
GATTACATGG CTCCGGAAGT GTTGACCGTG ATGAACGAGG ACCGAAGGGG CACATACGGC 
TTGGACTGTG ACTGGTGGTC TGTCGGAGTT GTTGCTTATG AGATGGTTTA TGGGAAGACC 
CCATTCACAG AGGGAACCTC CGCCCGGACC TTCAACAACA TCATGAACTT CCAGCGGTTT 
TTGAAGTTCC CAGATGACCC CAAAGTTAGC AGTGAGCTCC TTGATCTGCT TCAGAGTCTG 
CTGTGTGTCC AGAAAGAGAG ACTGAAGTTC GAGGGTCTCT GCTGCCACCC TTTCTTTGCC 
AGAACGGACT GGAACAACAT CCGTAACTCT CCTCCCCCCT TCGTCCCCAC CCTCAAGTCT 
GACGATGACA CCTCCAATTT TGATGAACCA GAGAAGAATT CGTGGGTTTC ATCCTCTGTG 
TGCCAGCTGA GCCCCTCGGG CTTCTCAGGC GAAGAGCTGC CGTTTGTGGG ATTTTCGTAC 
AGCAAGGCAC TGGGGTATCT TGGTAGATCT GAGTCTGTCG TGTCGAGTCT GGACTCCCCT 
GCCAAGGTTA GCTCCATGGA AAAGAAACTT CTCATCAAAA GCAAAGAGCT CCAAGACTCC 
CAGGACAAGT GTCACAAGAT GGAGCAGGAA ATGACCCGGT TACATCGCAG AGTGTCAGAG 
GTGGAGGCTG TGCTTAGTCA GAAGGAGGTG GAGCTGAAGG CCTCTGAGAC TCAGAGATCC 
CTCCTGGAGC AGGACCTTGC TACCTACATC ACAGAATGCA GTAGCTTAAA GCGAAGTTTG 
GAGCAAGCGC GGATGGAGGT GTCCCAGGAG GATGACAAAG CTCTGCAGCT TCTCCACGAC 
ATCCGAGAGC AGAGCCGGAA GCTCCAGGAG ATCAAGGAGC AGGAGTACCA GGCTCAGGTG 
GAGGAGATGA GGCTGATGAT GAATCAGCTG GAAGAAGACC TGGTGTCAGC CCGCAGACGC 
AGCGATCTCT ACGAGTCTGA GCTGAGGGAG TCTCGGCTTG CCGCCGAGGA ATTCAAGCGG 
AAGGCAAACG AATGTCAGCA CAAACTGATG AAGGTAGTCA GCCACCCTCC AAGAGGGGAC 
TCCGGGGGCA CGGCCCTGGA TGATCTTCAC AAGATGCAAG GCCACGCTGG CTTAACGAGT 
GCTAAGGACC AAGGGAAGCC TGAAGTGGGA GAATATTCCA AACTGGAGAA GATCAATGCT 
GAGCAGCAGC TGAAGATCCA GGAGCTCCAG GAGAAGCTGG AGAAGGCTGT AAAAGCCAGC 
ACAGAGGCCA CCGAGCTCCT GCAGAATATC CGCCAGGCAA AGGAGCGAGC AGAGCGGGAG 
CTAGAGAAGC TACACAACCG GGAAGACTCC TCCGAGGGCA TCAAAAAGAA GCTGGTGGAA 
GCCGAGGAAC GCCGCCACTC CCTGGAGAAC AAGGTAAAGA GACTAGAGAC CATGGAGCGT 
AGAGAGAACA GACTGAAGGA TGACATCCAG ACAAAGTCCG AACAGATCCA GCAGATGGCT 
GATAAAATTC TGGAGCTGGA GGAGAAACAT CGGGAGGCTC AGGTCTCAGC TCAACATCTA 
GAAGTACACT TGAAACAGAA AGAACAGCAC TACGAGGAAA AGATCAAGGT ATTGGACAAT 
CAGATAAAGA AGGACCTGGC CGACAAGGAG AGCCTGGAGA ACATGATGCA GAGACACGAG 
GAGGAGGCCC ACGAGAAGGG CAAGATCCTC AGCGAGCAGA AGGCGATGAT CAACGCGATG 
GATTCCAAGA TCCGATCCCT GGAGCAGAGG ATCGTGGAGC TGTCGGAAGC CAACAAGCTT 
GCGGCAAACA GCAGTCTCTT CACCCAGAGG AACATGAAGG CCCAGGAAGA GATGATCTCA 
GAACTCAGGC AGCAGAAATT TTACCTGGAG ACGCAGGCCG GAAAGCTGGA GGCCCAGAAC 
CGAAAGCTGG AAGAGCAACT GGAGAAGATC AGCCACCAAG ATCACAGTGA CAAGAGTCGG 
CTGCTGGAGC TGGAAACAAG GCTGAGGGAG GTCAGCCTGG AGCACGAGGA GCAGAAGCTG 
GAGCTGAAGC GGCAGCTCAC GGAGCTGCAG CTGTCCCTGC AGGAGCGCGA GTCCCAGCTG 
ACGGCCCTGC AGGCCGCCCG GGCAGCGCTG GAGAGCCAGC TCCGCCAGGC GAAGACAGAG 
CTGGAGGAGA CAACCGCGGA AGCGGAGGAG GAGATCCAGG CGCTCACGGC ACATCGAGAT 
GAGATCCAGC GCAAATTCGA TGCCCTTCGC AACAGCTGCA CGGTCATCAC CGACCTGGAG 
GAGCAGCTGA ACCAGCTCAC CGAGGACAAC GCCGAGCTCA ACAACCAAAA CTTCTACCTG 
TCCAAACAAC TCGATGAGGC TTCCGGGGCC AATGACGAGA TTGTGCAGCT GCGGAGTGAG 
GTGGACCATC TCCGCCGTGA GATCACGGAG CGGGAGATGC AGCTCACCAG CCAGAAGCAA 
GCACAACTCA GTGCTCCTGA TTTGCAGACG ATGGAGGCTC TGAAGACGAC ATGCACCATG 
CTGGAGGAGC AGGTCCTGGA CCTGGAGGCC CTGAACGACG AGCTGCTGGA GAAGGAGCGC 
CAGTGGGAGG CCTGGCGGAG CGTCCTCGGC GACGAGAAGT CCCAGTTCGA GTGTCGAGTT 
CGAGAGCTAC AGAGGATGCT GGACACCGAG AAGCAGAGCA GGGCTAGGGC CGATCAGCGG 
ATCACCGAGT CGCGCCAGGT GGTGGAGTTG GCGGTGAAGG AACACAAGGC CGAGATTCTT 
GCTCTGCAGC AGGCTCTCAA GGAGCAGAAG CTCAAAGCCG AGAGCCTGTC GGACAAGCTC 
AACGACCTGG AGAAGAAGCA CGCCATGCTG GAGATGAACG CCCGGAGCTT ACAGCAGAAA 
CTAGAGACAG AGCGGGAGCT CAAACAGAGG CTTCTGGAGG AGCAAGCCAA ATTGCAGCAG 
CAGATGGACC TGCAGAAGAA CCACATCTTC AGACTGACGC AAGGGCTGCA GGAGGCGCTG 
GACCGGGCGG ATCTGCTGAA GACAGAAAGG AGCGACCTGG AGTACCAGCT GGAAAACATT 
CAGGTTCTCT ACTCTCACGA GAAAGTGAAA ATGGAAGGCA CAATCTCTCA GCAAACCAAA 
CTCATTGATT TCCTGCAAGC CAAAATGGAC CAGCCTGCTA AAAAGAAAAA GGGTTTATTT 
AGTCGACGGA AAGAGGACCC TGCTTTGCCC ACACAGGTTC CTCTGCAGTA CAATGAGCTG 
AAGCTAGCCC TGGAAAAGGA GAAAGCCCGA TGCGCGGAGC TGGAGGAGGC CCTTCAGAAG 
ACCCGCATCG AACTCCGCTC TGCGCGGGAG GAAGCTGCCC ACCGCAAAGC CACGGACCAC 
CCGCACCCGT CTACGCCAGC CACTGCGAGG CAGCAGATCG CCATGTCTGC CATTGTGCGG 
TCGCCCGAGC ACCAGCCCAG TGCCATGAGC CTGCTTGCCC CACCATCCAG CCGCAGAAAG 
GAGTCGTCAA CTCCAGAGGA ATTCAGCCGG CGTCTGAAAG AGCGCATGCA CCACAACATC 
CCTCACCGGT TTAATGTGGG CCTGAACATG AGAGCCACCA AGTGCGCCGT GTGTCTGGAT 
ACTGTGCACT TTGGACGCCA GGCATCCAAA TGCCTCGAAT GTCAAGTGAT GTGTCACCCC 
AAATGCTCCA CCTGCTTGCC CGCTACCTGT GGCCTGCCAG CTGAATATGC CACACACTTC 
ACTGAGGCCT TCTGCCGGGA CAAAATGAAC TCCCCGGGGC TCCAGAGCAA GGAGCCTGGC 
AGCAGCTTGC ACCTGGAAGG GTGGATGAAA GTGCCCAGAA ATAACAAACG GGGACAGCAA 
GGCTGGGACA GGAAGTACAT TGTTCTGGAG GGGTCAAAAG TCCTCATCTA TGACAATGAA 
GCCAGAGAAG CTGGACAGAG GCCGGTGGAA GAATTTGAGC TGTGCCTTCC TGACGGGGAC 
GTATCTATTC ATGGTGCCGT TGGTGCTTCA GAACTTGCAA ATACCGCCAA AGCAGATGTC 
CCATACATCC TGAAGATGGA GTCTCACCCA CACACCACCT GCTGGCCTGG GAGAACCCTC 
TACTTGCTAG CACCCAGCTT CCCCGACAAG CAGCGCTGGG TCACCGCCTT AGAATCTGTC 
GTCGCAGGTG GGAGAGTTTC TAGGGAAAAG GCCGAAGCCG ATGCTGCACG TGACCATACT 
TCGAGTGAGC ATCAGCCAGT GTGGGTTGAG AAATTACTTG GAAACTCTCT GCTGAAACTG 
GAGGGCGATG ACCGGCTTGA CATGAACTGC ACCCTGCCCT TCAGTGACCA GGTAGTGCTG 
GTGGGCACGG AGGAAGGCCT CTACGCCCTG AATGTCTTGA AAAACTCCTT AACCCACATC 
CCAGGGATTG GCGCAGTCTT CCAAATTTAC ATCATCAAGG ACCTGGAGAA GCTGCTCATG 
ATAGCAGGGG AAGAGCGGGC TCTGTGCCTG GTGGACGTGA AGAAGGTGAA GCAGTCCCTG 
GCGCAGTCAC ACCTGCCTGC CCAGCCCGAC GTCTCCCCCA ACATATTCGA AGCCGTCAAA 
GGCTGCCACT TGTTCGCTGC TGGCAAGATC GAGAACAGCC TGTGCATCTG CGCCGCTATG 
CCAAGCAAAG TCGTCATCCT CCGCTACAAT GACAACCTCA GCAAGTACTG CATCCGCAAG 
GAGATCGAGA CCTCAGAGCC CTGCAGCTGT ATCCACTTCA CCAACTACAG CATCCTCATT 
GGGACCAACA AATTCTATGA GATCGACATG AAGCAGTACA CGCTTGACGA GTTCCTGGAC 
AAGAACGACC ATTCCTTGGC ACCTGCTGTG TTCGCCTCCT CGTCCAACAG CTTCCCTGTC 
TCCATTGTGC AGGCGAACAG CGCCGGGCAG CGAGAAGAAT ACCTGCTGTG CTTCCACGAA 
TTTGGGGTGT TCGTGGATTC TTACGGAAGA CGTAGCCGCA CAGATGATCT CAAGTGGAGT 
CGCTTACCTC TGGCCTTCGC CTACAGAGAA CCTTATCTGT TTGTGACTCA CTTCAACTCC 
CTGGAAGTCA TTGAGATCCA GGCCAGATCC TCACTGGGGA GCCCTGCCCG AGCATATCTG 
GAAATTCCAA ACCCTCGCTA CCTGGGCCCC GCGATTTCCT CCGGAGCGAT TTACCTGGCC 
TCCTCATACC AGGACAAGTT AAGGGTCATA TGCTGCAAAG GAAACCTCGT GAAGGAGTCA 
GGCACTGAGC AGCACCGGGT GCCCTCCACC TCCCGCAGCT CCTTCACTGC CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cit ( XM_030254098.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030109958.1 Mus musculus citron (Cit), transcript variant X9, mRNA.

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CloneID	OMu88722
Clone ID Related Accession (Same CDS sequence)	XM_030254098.1
Accession Version	XM_030254098.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6300bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	citron Rho-interacting kinase isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000071.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTGAAGT TCAAGTATGG TGTGCGGAAC CCGCCGGAGG CCAGTGCCTC CGAGCCCATT 
GCCAGTCGGG CCTCCAGGCT AAATCTCTTC TTCCAGGGGA AACCGCCCCT CATGACTCAA 
CAGCAGATGT CTGCTCTTTC CCGGGAAGGG ATGCTAGACG CCCTCTTCGC TCTCTTTGAA 
GAGTGCAGCC AACCCGCCCT GATGAAGATG AAGCACGTGA GCAGCTTTGT CCAGAAGTAT 
TCCGACACCA TAGCCGAGTT GCGGGAGCTG CAGCCGTCGG CGAGAGACTT CGAAGTTCGA 
AGCCTTGTGG GCTGTGGTCA CTTCGCTGAA GTGCAGGTGG TTAGAGAGAA GGCGACCGGG 
GACGTCTATG CCATGAAAAT CATGAAGAAG AAGGCTTTGC TGGCCCAGGA ACAGGTTTCA 
TTTTTCGAGG AGGAGAGGAA CATATTATCT CGGAGCACGA GTCCTTGGAT CCCCCAGTTA 
CAGTACGCCT TTCAGGACAA AAATAACCTT TACCTGGTCA TGGAATATCA GCCTGGAGGG 
GATTTGCTGT CGCTTCTGAA CAGATACGAG GACCAATTAG ATGAGAGCAT GATCCAGTTT 
TACCTTGCTG AGCTGATTTT GGCTGTCCAC AGCGTGCACC AGATGGGATA TGTGCATCGA 
GACATCAAGC CCGAGAACAT CCTCATCGAC CGGACGGGAC ACATCAAGCT GGTGGATTTT 
GGATCAGCCG CTAAGATGAA TTCAAATAAG GTGGATGCCA AACTCCCCAT TGGGACCCCG 
GATTACATGG CTCCGGAAGT GTTGACCGTG ATGAACGAGG ACCGAAGGGG CACATACGGC 
TTGGACTGTG ACTGGTGGTC TGTCGGAGTT GTTGCTTATG AGATGGTTTA TGGGAAGACC 
CCATTCACAG AGGGAACCTC CGCCCGGACC TTCAACAACA TCATGAACTT CCAGCGGTTT 
TTGAAGTTCC CAGATGACCC CAAAGTTAGC AGTGAGCTCC TTGATCTGCT TCAGAGTCTG 
CTGTGTGTCC AGAAAGAGAG ACTGAAGTTC GAGGGTCTCT GCTGCCACCC TTTCTTTGCC 
AGAACGGACT GGAACAACAT CCGTAACTCT CCTCCCCCCT TCGTCCCCAC CCTCAAGTCT 
GACGATGACA CCTCCAATTT TGATGAACCA GAGAAGAATT CGTGGGTTTC ATCCTCTGTG 
TGCCAGCTGA GCCCCTCGGG CTTCTCAGGC GAAGAGCTGC CGTTTGTGGG ATTTTCGTAC 
AGCAAGGCAC TGGGGTATCT TGGTAGATCT GAGTCTGTCG TGTCGAGTCT GGACTCCCCT 
GCCAAGGTTA GCTCCATGGA AAAGAAACTT CTCATCAAAA GCAAAGAGCT CCAAGACTCC 
CAGGACAAGT GTCACAAGAT GGAGCAGGAA ATGACCCGGT TACATCGCAG AGTGTCAGAG 
GTGGAGGCTG TGCTTAGTCA GAAGGAGGTG GAGCTGAAGG CCTCTGAGAC TCAGAGATCC 
CTCCTGGAGC AGGACCTTGC TACCTACATC ACAGAATGCA GTAGCTTAAA GCGAAGTTTG 
GAGCAAGCGC GGATGGAGGT GTCCCAGGAG GATGACAAAG CTCTGCAGCT TCTCCACGAC 
ATCCGAGAGC AGAGCCGGAA GCTCCAGGAG ATCAAGGAGC AGGAGTACCA GGCTCAGGTG 
GAGGAGATGA GGCTGATGAT GAATCAGCTG GAAGAAGACC TGGTGTCAGC CCGCAGACGC 
AGCGATCTCT ACGAGTCTGA GCTGAGGGAG TCTCGGCTTG CCGCCGAGGA ATTCAAGCGG 
AAGGCAAACG AATGTCAGCA CAAACTGATG AAGGTAGTCA GCCACCCTCC AAGAGGGGAC 
TCCGGGGGCA CGGCCCTGGA TGATCTTCAC AAGATGCAAG GCCACGCTGG CTTAACGAGT 
GCTAAGGACC AAGGGAAGCC TGAAGTGGGA GAATATTCCA AACTGGAGAA GATCAATGCT 
GAGCAGCAGC TGAAGATCCA GGAGCTCCAG GAGAAGCTGG AGAAGGCTGT AAAAGCCAGC 
ACAGAGGCCA CCGAGCTCCT GCAGAATATC CGCCAGGCAA AGGAGCGAGC AGAGCGGGAG 
CTAGAGAAGC TACACAACCG GGAAGACTCC TCCGAGGGCA TCAAAAAGAA GCTGGTGGAA 
GCCGAGGAAC GCCGCCACTC CCTGGAGAAC AAGGTAAAGA GACTAGAGAC CATGGAGCGT 
AGAGAGAACA GACTGAAGGA TGACATCCAG ACAAAGTCCG AACAGATCCA GCAGATGGCT 
GATAAAATTC TGGAGCTGGA GGAGAAACAT CGGGAGGCTC AGGTCTCAGC TCAACATCTA 
GAAGTACACT TGAAACAGAA AGAACAGCAC TACGAGGAAA AGATCAAGGT ATTGGACAAT 
CAGATAAAGA AGGACCTGGC CGACAAGGAG AGCCTGGAGA ACATGATGCA GAGACACGAG 
GAGGAGGCCC ACGAGAAGGG CAAGATCCTC AGCGAGCAGA AGGCGATGAT CAACGCGATG 
GATTCCAAGA TCCGATCCCT GGAGCAGAGG ATCGTGGAGC TGTCGGAAGC CAACAAGCTT 
GCGGCAAACA GCAGTCTCTT CACCCAGAGG AACATGAAGG CCCAGGAAGA GATGATCTCA 
GAACTCAGGC AGCAGAAATT TTACCTGGAG ACGCAGGCCG GAAAGCTGGA GGCCCAGAAC 
CGAAAGCTGG AAGAGCAACT GGAGAAGATC AGCCACCAAG ATCACAGTGA CAAGAGTCGG 
CTGCTGGAGC TGGAAACAAG GCTGAGGGAG GTCAGCCTGG AGCACGAGGA GCAGAAGCTG 
GAGCTGAAGC GGCAGCTCAC GGAGCTGCAG CTGTCCCTGC AGGAGCGCGA GTCCCAGCTG 
ACGGCCCTGC AGGCCGCCCG GGCAGCGCTG GAGAGCCAGC TCCGCCAGGC GAAGACAGAG 
CTGGAGGAGA CAACCGCGGA AGCGGAGGAG GAGATCCAGG CGCTCACGGC ACATCGAGAT 
GAGATCCAGC GCAAATTCGA TGCCCTTCGC AACAGCTGCA CGGTCATCAC CGACCTGGAG 
GAGCAGCTGA ACCAGCTCAC CGAGGACAAC GCCGAGCTCA ACAACCAAAA CTTCTACCTG 
TCCAAACAAC TCGATGAGGC TTCCGGGGCC AATGACGAGA TTGTGCAGCT GCGGAGTGAG 
GTGGACCATC TCCGCCGTGA GATCACGGAG CGGGAGATGC AGCTCACCAG CCAGAAGCAA 
ACGATGGAGG CTCTGAAGAC GACATGCACC ATGCTGGAGG AGCAGGTCCT GGACCTGGAG 
GCCCTGAACG ACGAGCTGCT GGAGAAGGAG CGCCAGTGGG AGGCCTGGCG GAGCGTCCTC 
GGCGACGAGA AGTCCCAGTT CGAGTGTCGA GTTCGAGAGC TACAGAGGAT GCTGGACACC 
GAGAAGCAGA GCAGGGCTAG GGCCGATCAG CGGATCACCG AGTCGCGCCA GGTGGTGGAG 
TTGGCGGTGA AGGAACACAA GGCCGAGATT CTTGCTCTGC AGCAGGCTCT CAAGGAGCAG 
AAGCTCAAAG CCGAGAGCCT GTCGGACAAG CTCAACGACC TGGAGAAGAA GCACGCCATG 
CTGGAGATGA ACGCCCGGAG CTTACAGCAG AAACTAGAGA CAGAGCGGGA GCTCAAACAG 
AGGCTTCTGG AGGAGCAAGC CAAATTGCAG CAGCAGATGG ACCTGCAGAA GAACCACATC 
TTCAGACTGA CGCAAGGGCT GCAGGAGGCG CTGGACCGGG CGGATCTGCT GAAGACAGAA 
AGGAGCGACC TGGAGTACCA GCTGGAAAAC ATTCAGGTTC TCTACTCTCA CGAGAAAGTG 
AAAATGGAAG GCACAATCTC TCAGCAAACC AAACTCATTG ATTTCCTGCA AGCCAAAATG 
GACCAGCCTG CTAAAAAGAA AAAGGTTCCT CTGCAGTACA ATGAGCTGAA GCTAGCCCTG 
GAAAAGGAGA AAGCCCGATG CGCGGAGCTG GAGGAGGCCC TTCAGAAGAC CCGCATCGAA 
CTCCGCTCTG CGCGGGAGGA AGCTGCCCAC CGCAAAGCCA CGGACCACCC GCACCCGTCT 
ACGCCAGCCA CTGCGAGGCA GCAGATCGCC ATGTCTGCCA TTGTGCGGTC GCCCGAGCAC 
CAGCCCAGTG CCATGAGCCT GCTTGCCCCA CCATCCAGCC GCAGAAAGGA GTCGTCAACT 
CCAGAGGAAT TCAGCCGGCG TCTGAAAGAG CGCATGCACC ACAACATCCC TCACCGGTTT 
AATGTGGGCC TGAACATGAG AGCCACCAAG TGCGCCGTGT GTCTGGATAC TGTGCACTTT 
GGACGCCAGG CATCCAAATG CCTCGAATGT CAAGTGATGT GTCACCCCAA ATGCTCCACC 
TGCTTGCCCG CTACCTGTGG CCTGCCAGCT GAATATGCCA CACACTTCAC TGAGGCCTTC 
TGCCGGGACA AAATGAACTC CCCGGGGCTC CAGAGCAAGG AGCCTGGCAG CAGCTTGCAC 
CTGGAAGGGT GGATGAAAGT GCCCAGAAAT AACAAACGGG GACAGCAAGG CTGGGACAGG 
AAGTACATTG TTCTGGAGGG GTCAAAAGTC CTCATCTATG ACAATGAAGC CAGAGAAGCT 
GGACAGAGGC CGGTGGAAGA ATTTGAGCTG TGCCTTCCTG ACGGGGACGT ATCTATTCAT 
GGTGCCGTTG GTGCTTCAGA ACTTGCAAAT ACCGCCAAAG CAGATGTCCC ATACATCCTG 
AAGATGGAGT CTCACCCACA CACCACCTGC TGGCCTGGGA GAACCCTCTA CTTGCTAGCA 
CCCAGCTTCC CCGACAAGCA GCGCTGGGTC ACCGCCTTAG AATCTGTCGT CGCAGGTGGG 
AGAGTTTCTA GGGAAAAGGC CGAAGCCGAT GCTGCACGTG ACCATACTTC GAGTGAGCAT 
CAGCCAGTGT GGGTTGAGAA ATTACTTGGA AACTCTCTGC TGAAACTGGA GGGCGATGAC 
CGGCTTGACA TGAACTGCAC CCTGCCCTTC AGTGACCAGG TAGTGCTGGT GGGCACGGAG 
GAAGGCCTCT ACGCCCTGAA TGTCTTGAAA AACTCCTTAA CCCACATCCC AGGGATTGGC 
GCAGTCTTCC AAATTTACAT CATCAAGGAC CTGGAGAAGC TGCTCATGAT AGCAGGGGAA 
GAGCGGGCTC TGTGCCTGGT GGACGTGAAG AAGGTGAAGC AGTCCCTGGC GCAGTCACAC 
CTGCCTGCCC AGCCCGACGT CTCCCCCAAC ATATTCGAAG CCGTCAAAGG CTGCCACTTG 
TTCGCTGCTG GCAAGATCGA GAACAGCCTG TGCATCTGCG CCGCTATGCC AAGCAAAGTC 
GTCATCCTCC GCTACAATGA CAACCTCAGC AAGTACTGCA TCCGCAAGGA GATCGAGACC 
TCAGAGCCCT GCAGCTGTAT CCACTTCACC AACTACAGCA TCCTCATTGG GACCAACAAA 
TTCTATGAGA TCGACATGAA GCAGTACACG CTTGACGAGT TCCTGGACAA GAACGACCAT 
TCCTTGGCAC CTGCTGTGTT CGCCTCCTCG TCCAACAGCT TCCCTGTCTC CATTGTGCAG 
GCGAACAGCG CCGGGCAGCG AGAAGAATAC CTGCTGTGCT TCCACGAATT TGGGGTGTTC 
GTGGATTCTT ACGGAAGACG TAGCCGCACA GATGATCTCA AGTGGAGTCG CTTACCTCTG 
GCCTTCGCCT ACAGAGAACC TTATCTGTTT GTGACTCACT TCAACTCCCT GGAAGTCATT 
GAGATCCAGG CCAGATCCTC ACTGGGGAGC CCTGCCCGAG CATATCTGGA AATTCCAAAC 
CCTCGCTACC TGGGCCCCGC GATTTCCTCC GGAGCGATTT ACCTGGCCTC CTCATACCAG 
GACAAGTTAA GGGTCATATG CTGCAAAGGA AACCTCGTGA AGGAGTCAGG CACTGAGCAG 
CACCGGGTGC CCTCCACCTC CCGCAGCAGC CCCAACAAGC GAGGCCCACC AACATACAAC 
GAGCACATCA CCAAACGCGT GGCCTCCAGC CCGGCGCCAC CGGAAGGCCC CAGCCACCCC 
CGAGAGCCAA GCACACCGCA CCGCTACCGA GACAGAGAGG GTCGGACAGA GCTGCGCAGG 
GACAAGTCTC CAGGCCGCCC TCTGGAGCGG GAGAAGTCCC CAGGCCGAAT GCTCAGCACT 
AGGAGAGAGC GGTCCCCAGG GAGACTGTTT GAAGACAGCA GCAGGGGCCG GCTGCCTGCA 
GGAGCAGTGA GGACCCCACT GTCCCAGGTT AACAAGGTCT GGGACCAGTC TTCAGTATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030109958.1
CDS	157..6456
Translation	MLKFKYGVRNPPEASASEPIASRASRLNLFFQGKPPLMTQQQMSALSREGMLDALFALFEECSQPALMKMKHVSSFVQKYSDTIAELRELQPSARDFEVRSLVGCGHFAEVQVVREKATGDVYAMKIMKKKALLAQEQVSFFEEERNILSRSTSPWIPQLQYAFQDKNNLYLVMEYQPGGDLLSLLNRYEDQLDESMIQFYLAELILAVHSVHQMGYVHRDIKPENILIDRTGHIKLVDFGSAAKMNSNKVDAKLPIGTPDYMAPEVLTVMNEDRRGTYGLDCDWWSVGVVAYEMVYGKTPFTEGTSARTFNNIMNFQRFLKFPDDPKVSSELLDLLQSLLCVQKERLKFEGLCCHPFFARTDWNNIRNSPPPFVPTLKSDDDTSNFDEPEKNSWVSSSVCQLSPSGFSGEELPFVGFSYSKALGYLGRSESVVSSLDSPAKVSSMEKKLLIKSKELQDSQDKCHKMEQEMTRLHRRVSEVEAVLSQKEVELKASETQRSLLEQDLATYITECSSLKRSLEQARMEVSQEDDKALQLLHDIREQSRKLQEIKEQEYQAQVEEMRLMMNQLEEDLVSARRRSDLYESELRESRLAAEEFKRKANECQHKLMKVVSHPPRGDSGGTALDDLHKMQGHAGLTSAKDQGKPEVGEYSKLEKINAEQQLKIQELQEKLEKAVKASTEATELLQNIRQAKERAERELEKLHNREDSSEGIKKKLVEAEERRHSLENKVKRLETMERRENRLKDDIQTKSEQIQQMADKILELEEKHREAQVSAQHLEVHLKQKEQHYEEKIKVLDNQIKKDLADKESLENMMQRHEEEAHEKGKILSEQKAMINAMDSKIRSLEQRIVELSEANKLAANSSLFTQRNMKAQEEMISELRQQKFYLETQAGKLEAQNRKLEEQLEKISHQDHSDKSRLLELETRLREVSLEHEEQKLELKRQLTELQLSLQERESQLTALQAARAALESQLRQAKTELEETTAEAEEEIQALTAHRDEIQRKFDALRNSCTVITDLEEQLNQLTEDNAELNNQNFYLSKQLDEASGANDEIVQLRSEVDHLRREITEREMQLTSQKQTMEALKTTCTMLEEQVLDLEALNDELLEKERQWEAWRSVLGDEKSQFECRVRELQRMLDTEKQSRARADQRITESRQVVELAVKEHKAEILALQQALKEQKLKAESLSDKLNDLEKKHAMLEMNARSLQQKLETERELKQRLLEEQAKLQQQMDLQKNHIFRLTQGLQEALDRADLLKTERSDLEYQLENIQVLYSHEKVKMEGTISQQTKLIDFLQAKMDQPAKKKKVPLQYNELKLALEKEKARCAELEEALQKTRIELRSAREEAAHRKATDHPHPSTPATARQQIAMSAIVRSPEHQPSAMSLLAPPSSRRKESSTPEEFSRRLKERMHHNIPHRFNVGLNMRATKCAVCLDTVHFGRQASKCLECQVMCHPKCSTCLPATCGLPAEYATHFTEAFCRDKMNSPGLQSKEPGSSLHLEGWMKVPRNNKRGQQGWDRKYIVLEGSKVLIYDNEAREAGQRPVEEFELCLPDGDVSIHGAVGASELANTAKADVPYILKMESHPHTTCWPGRTLYLLAPSFPDKQRWVTALESVVAGGRVSREKAEADAARDHTSSEHQPVWVEKLLGNSLLKLEGDDRLDMNCTLPFSDQVVLVGTEEGLYALNVLKNSLTHIPGIGAVFQIYIIKDLEKLLMIAGEERALCLVDVKKVKQSLAQSHLPAQPDVSPNIFEAVKGCHLFAAGKIENSLCICAAMPSKVVILRYNDNLSKYCIRKEIETSEPCSCIHFTNYSILIGTNKFYEIDMKQYTLDEFLDKNDHSLAPAVFASSSNSFPVSIVQANSAGQREEYLLCFHEFGVFVDSYGRRSRTDDLKWSRLPLAFAYREPYLFVTHFNSLEVIEIQARSSLGSPARAYLEIPNPRYLGPAISSGAIYLASSYQDKLRVICCKGNLVKESGTEQHRVPSTSRSSPNKRGPPTYNEHITKRVASSPAPPEGPSHPREPSTPHRYRDREGRTELRRDKSPGRPLEREKSPGRMLSTRRERSPGRLFEDSSRGRLPAGAVRTPLSQVNKVWDQSSV

Target ORF information:

RefSeq Version	XM_030254098.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus citron (Cit), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030254098.1

ORF Insert Sequence:

ATGTTGAAGT TCAAGTATGG TGTGCGGAAC CCGCCGGAGG CCAGTGCCTC CGAGCCCATT 
GCCAGTCGGG CCTCCAGGCT AAATCTCTTC TTCCAGGGGA AACCGCCCCT CATGACTCAA 
CAGCAGATGT CTGCTCTTTC CCGGGAAGGG ATGCTAGACG CCCTCTTCGC TCTCTTTGAA 
GAGTGCAGCC AACCCGCCCT GATGAAGATG AAGCACGTGA GCAGCTTTGT CCAGAAGTAT 
TCCGACACCA TAGCCGAGTT GCGGGAGCTG CAGCCGTCGG CGAGAGACTT CGAAGTTCGA 
AGCCTTGTGG GCTGTGGTCA CTTCGCTGAA GTGCAGGTGG TTAGAGAGAA GGCGACCGGG 
GACGTCTATG CCATGAAAAT CATGAAGAAG AAGGCTTTGC TGGCCCAGGA ACAGGTTTCA 
TTTTTCGAGG AGGAGAGGAA CATATTATCT CGGAGCACGA GTCCTTGGAT CCCCCAGTTA 
CAGTACGCCT TTCAGGACAA AAATAACCTT TACCTGGTCA TGGAATATCA GCCTGGAGGG 
GATTTGCTGT CGCTTCTGAA CAGATACGAG GACCAATTAG ATGAGAGCAT GATCCAGTTT 
TACCTTGCTG AGCTGATTTT GGCTGTCCAC AGCGTGCACC AGATGGGATA TGTGCATCGA 
GACATCAAGC CCGAGAACAT CCTCATCGAC CGGACGGGAC ACATCAAGCT GGTGGATTTT 
GGATCAGCCG CTAAGATGAA TTCAAATAAG GTGGATGCCA AACTCCCCAT TGGGACCCCG 
GATTACATGG CTCCGGAAGT GTTGACCGTG ATGAACGAGG ACCGAAGGGG CACATACGGC 
TTGGACTGTG ACTGGTGGTC TGTCGGAGTT GTTGCTTATG AGATGGTTTA TGGGAAGACC 
CCATTCACAG AGGGAACCTC CGCCCGGACC TTCAACAACA TCATGAACTT CCAGCGGTTT 
TTGAAGTTCC CAGATGACCC CAAAGTTAGC AGTGAGCTCC TTGATCTGCT TCAGAGTCTG 
CTGTGTGTCC AGAAAGAGAG ACTGAAGTTC GAGGGTCTCT GCTGCCACCC TTTCTTTGCC 
AGAACGGACT GGAACAACAT CCGTAACTCT CCTCCCCCCT TCGTCCCCAC CCTCAAGTCT 
GACGATGACA CCTCCAATTT TGATGAACCA GAGAAGAATT CGTGGGTTTC ATCCTCTGTG 
TGCCAGCTGA GCCCCTCGGG CTTCTCAGGC GAAGAGCTGC CGTTTGTGGG ATTTTCGTAC 
AGCAAGGCAC TGGGGTATCT TGGTAGATCT GAGTCTGTCG TGTCGAGTCT GGACTCCCCT 
GCCAAGGTTA GCTCCATGGA AAAGAAACTT CTCATCAAAA GCAAAGAGCT CCAAGACTCC 
CAGGACAAGT GTCACAAGAT GGAGCAGGAA ATGACCCGGT TACATCGCAG AGTGTCAGAG 
GTGGAGGCTG TGCTTAGTCA GAAGGAGGTG GAGCTGAAGG CCTCTGAGAC TCAGAGATCC 
CTCCTGGAGC AGGACCTTGC TACCTACATC ACAGAATGCA GTAGCTTAAA GCGAAGTTTG 
GAGCAAGCGC GGATGGAGGT GTCCCAGGAG GATGACAAAG CTCTGCAGCT TCTCCACGAC 
ATCCGAGAGC AGAGCCGGAA GCTCCAGGAG ATCAAGGAGC AGGAGTACCA GGCTCAGGTG 
GAGGAGATGA GGCTGATGAT GAATCAGCTG GAAGAAGACC TGGTGTCAGC CCGCAGACGC 
AGCGATCTCT ACGAGTCTGA GCTGAGGGAG TCTCGGCTTG CCGCCGAGGA ATTCAAGCGG 
AAGGCAAACG AATGTCAGCA CAAACTGATG AAGGTAGTCA GCCACCCTCC AAGAGGGGAC 
TCCGGGGGCA CGGCCCTGGA TGATCTTCAC AAGATGCAAG GCCACGCTGG CTTAACGAGT 
GCTAAGGACC AAGGGAAGCC TGAAGTGGGA GAATATTCCA AACTGGAGAA GATCAATGCT 
GAGCAGCAGC TGAAGATCCA GGAGCTCCAG GAGAAGCTGG AGAAGGCTGT AAAAGCCAGC 
ACAGAGGCCA CCGAGCTCCT GCAGAATATC CGCCAGGCAA AGGAGCGAGC AGAGCGGGAG 
CTAGAGAAGC TACACAACCG GGAAGACTCC TCCGAGGGCA TCAAAAAGAA GCTGGTGGAA 
GCCGAGGAAC GCCGCCACTC CCTGGAGAAC AAGGTAAAGA GACTAGAGAC CATGGAGCGT 
AGAGAGAACA GACTGAAGGA TGACATCCAG ACAAAGTCCG AACAGATCCA GCAGATGGCT 
GATAAAATTC TGGAGCTGGA GGAGAAACAT CGGGAGGCTC AGGTCTCAGC TCAACATCTA 
GAAGTACACT TGAAACAGAA AGAACAGCAC TACGAGGAAA AGATCAAGGT ATTGGACAAT 
CAGATAAAGA AGGACCTGGC CGACAAGGAG AGCCTGGAGA ACATGATGCA GAGACACGAG 
GAGGAGGCCC ACGAGAAGGG CAAGATCCTC AGCGAGCAGA AGGCGATGAT CAACGCGATG 
GATTCCAAGA TCCGATCCCT GGAGCAGAGG ATCGTGGAGC TGTCGGAAGC CAACAAGCTT 
GCGGCAAACA GCAGTCTCTT CACCCAGAGG AACATGAAGG CCCAGGAAGA GATGATCTCA 
GAACTCAGGC AGCAGAAATT TTACCTGGAG ACGCAGGCCG GAAAGCTGGA GGCCCAGAAC 
CGAAAGCTGG AAGAGCAACT GGAGAAGATC AGCCACCAAG ATCACAGTGA CAAGAGTCGG 
CTGCTGGAGC TGGAAACAAG GCTGAGGGAG GTCAGCCTGG AGCACGAGGA GCAGAAGCTG 
GAGCTGAAGC GGCAGCTCAC GGAGCTGCAG CTGTCCCTGC AGGAGCGCGA GTCCCAGCTG 
ACGGCCCTGC AGGCCGCCCG GGCAGCGCTG GAGAGCCAGC TCCGCCAGGC GAAGACAGAG 
CTGGAGGAGA CAACCGCGGA AGCGGAGGAG GAGATCCAGG CGCTCACGGC ACATCGAGAT 
GAGATCCAGC GCAAATTCGA TGCCCTTCGC AACAGCTGCA CGGTCATCAC CGACCTGGAG 
GAGCAGCTGA ACCAGCTCAC CGAGGACAAC GCCGAGCTCA ACAACCAAAA CTTCTACCTG 
TCCAAACAAC TCGATGAGGC TTCCGGGGCC AATGACGAGA TTGTGCAGCT GCGGAGTGAG 
GTGGACCATC TCCGCCGTGA GATCACGGAG CGGGAGATGC AGCTCACCAG CCAGAAGCAA 
ACGATGGAGG CTCTGAAGAC GACATGCACC ATGCTGGAGG AGCAGGTCCT GGACCTGGAG 
GCCCTGAACG ACGAGCTGCT GGAGAAGGAG CGCCAGTGGG AGGCCTGGCG GAGCGTCCTC 
GGCGACGAGA AGTCCCAGTT CGAGTGTCGA GTTCGAGAGC TACAGAGGAT GCTGGACACC 
GAGAAGCAGA GCAGGGCTAG GGCCGATCAG CGGATCACCG AGTCGCGCCA GGTGGTGGAG 
TTGGCGGTGA AGGAACACAA GGCCGAGATT CTTGCTCTGC AGCAGGCTCT CAAGGAGCAG 
AAGCTCAAAG CCGAGAGCCT GTCGGACAAG CTCAACGACC TGGAGAAGAA GCACGCCATG 
CTGGAGATGA ACGCCCGGAG CTTACAGCAG AAACTAGAGA CAGAGCGGGA GCTCAAACAG 
AGGCTTCTGG AGGAGCAAGC CAAATTGCAG CAGCAGATGG ACCTGCAGAA GAACCACATC 
TTCAGACTGA CGCAAGGGCT GCAGGAGGCG CTGGACCGGG CGGATCTGCT GAAGACAGAA 
AGGAGCGACC TGGAGTACCA GCTGGAAAAC ATTCAGGTTC TCTACTCTCA CGAGAAAGTG 
AAAATGGAAG GCACAATCTC TCAGCAAACC AAACTCATTG ATTTCCTGCA AGCCAAAATG 
GACCAGCCTG CTAAAAAGAA AAAGGTTCCT CTGCAGTACA ATGAGCTGAA GCTAGCCCTG 
GAAAAGGAGA AAGCCCGATG CGCGGAGCTG GAGGAGGCCC TTCAGAAGAC CCGCATCGAA 
CTCCGCTCTG CGCGGGAGGA AGCTGCCCAC CGCAAAGCCA CGGACCACCC GCACCCGTCT 
ACGCCAGCCA CTGCGAGGCA GCAGATCGCC ATGTCTGCCA TTGTGCGGTC GCCCGAGCAC 
CAGCCCAGTG CCATGAGCCT GCTTGCCCCA CCATCCAGCC GCAGAAAGGA GTCGTCAACT 
CCAGAGGAAT TCAGCCGGCG TCTGAAAGAG CGCATGCACC ACAACATCCC TCACCGGTTT 
AATGTGGGCC TGAACATGAG AGCCACCAAG TGCGCCGTGT GTCTGGATAC TGTGCACTTT 
GGACGCCAGG CATCCAAATG CCTCGAATGT CAAGTGATGT GTCACCCCAA ATGCTCCACC 
TGCTTGCCCG CTACCTGTGG CCTGCCAGCT GAATATGCCA CACACTTCAC TGAGGCCTTC 
TGCCGGGACA AAATGAACTC CCCGGGGCTC CAGAGCAAGG AGCCTGGCAG CAGCTTGCAC 
CTGGAAGGGT GGATGAAAGT GCCCAGAAAT AACAAACGGG GACAGCAAGG CTGGGACAGG 
AAGTACATTG TTCTGGAGGG GTCAAAAGTC CTCATCTATG ACAATGAAGC CAGAGAAGCT 
GGACAGAGGC CGGTGGAAGA ATTTGAGCTG TGCCTTCCTG ACGGGGACGT ATCTATTCAT 
GGTGCCGTTG GTGCTTCAGA ACTTGCAAAT ACCGCCAAAG CAGATGTCCC ATACATCCTG 
AAGATGGAGT CTCACCCACA CACCACCTGC TGGCCTGGGA GAACCCTCTA CTTGCTAGCA 
CCCAGCTTCC CCGACAAGCA GCGCTGGGTC ACCGCCTTAG AATCTGTCGT CGCAGGTGGG 
AGAGTTTCTA GGGAAAAGGC CGAAGCCGAT GCTGCACGTG ACCATACTTC GAGTGAGCAT 
CAGCCAGTGT GGGTTGAGAA ATTACTTGGA AACTCTCTGC TGAAACTGGA GGGCGATGAC 
CGGCTTGACA TGAACTGCAC CCTGCCCTTC AGTGACCAGG TAGTGCTGGT GGGCACGGAG 
GAAGGCCTCT ACGCCCTGAA TGTCTTGAAA AACTCCTTAA CCCACATCCC AGGGATTGGC 
GCAGTCTTCC AAATTTACAT CATCAAGGAC CTGGAGAAGC TGCTCATGAT AGCAGGGGAA 
GAGCGGGCTC TGTGCCTGGT GGACGTGAAG AAGGTGAAGC AGTCCCTGGC GCAGTCACAC 
CTGCCTGCCC AGCCCGACGT CTCCCCCAAC ATATTCGAAG CCGTCAAAGG CTGCCACTTG 
TTCGCTGCTG GCAAGATCGA GAACAGCCTG TGCATCTGCG CCGCTATGCC AAGCAAAGTC 
GTCATCCTCC GCTACAATGA CAACCTCAGC AAGTACTGCA TCCGCAAGGA GATCGAGACC 
TCAGAGCCCT GCAGCTGTAT CCACTTCACC AACTACAGCA TCCTCATTGG GACCAACAAA 
TTCTATGAGA TCGACATGAA GCAGTACACG CTTGACGAGT TCCTGGACAA GAACGACCAT 
TCCTTGGCAC CTGCTGTGTT CGCCTCCTCG TCCAACAGCT TCCCTGTCTC CATTGTGCAG 
GCGAACAGCG CCGGGCAGCG AGAAGAATAC CTGCTGTGCT TCCACGAATT TGGGGTGTTC 
GTGGATTCTT ACGGAAGACG TAGCCGCACA GATGATCTCA AGTGGAGTCG CTTACCTCTG 
GCCTTCGCCT ACAGAGAACC TTATCTGTTT GTGACTCACT TCAACTCCCT GGAAGTCATT 
GAGATCCAGG CCAGATCCTC ACTGGGGAGC CCTGCCCGAG CATATCTGGA AATTCCAAAC 
CCTCGCTACC TGGGCCCCGC GATTTCCTCC GGAGCGATTT ACCTGGCCTC CTCATACCAG 
GACAAGTTAA GGGTCATATG CTGCAAAGGA AACCTCGTGA AGGAGTCAGG CACTGAGCAG 
CACCGGGTGC CCTCCACCTC CCGCAGCAGC CCCAACAAGC GAGGCCCACC AACATACAAC 
GAGCACATCA CCAAACGCGT GGCCTCCAGC CCGGCGCCAC CGGAAGGCCC CAGCCACCCC 
CGAGAGCCAA GCACACCGCA CCGCTACCGA GACAGAGAGG GTCGGACAGA GCTGCGCAGG 
GACAAGTCTC CAGGCCGCCC TCTGGAGCGG GAGAAGTCCC CAGGCCGAAT GCTCAGCACT 
AGGAGAGAGC GGTCCCCAGG GAGACTGTTT GAAGACAGCA GCAGGGGCCG GCTGCCTGCA 
GGAGCAGTGA GGACCCCACT GTCCCAGGTT AACAAGGTCT GGGACCAGTC TTCAGTATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cit ( XM_030254100.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030109960.1 Mus musculus citron (Cit), transcript variant X12, mRNA.

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CloneID	OMu88723
Clone ID Related Accession (Same CDS sequence)	XM_030254100.1
Accession Version	XM_030254100.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6282bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	citron Rho-interacting kinase isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000071.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTGAAGT TCAAGTATGG TGTGCGGAAC CCGCCGGAGG CCAGTGCCTC CGAGCCCATT 
GCCAGTCGGG CCTCCAGGCT AAATCTCTTC TTCCAGGGGA AACCGCCCCT CATGACTCAA 
CAGCAGATGT CTGCTCTTTC CCGGGAAGGG ATGCTAGACG CCCTCTTCGC TCTCTTTGAA 
GAGTGCAGCC AACCCGCCCT GATGAAGATG AAGCACGTGA GCAGCTTTGT CCAGAAGTAT 
TCCGACACCA TAGCCGAGTT GCGGGAGCTG CAGCCGTCGG CGAGAGACTT CGAAGTTCGA 
AGCCTTGTGG GCTGTGGTCA CTTCGCTGAA GTGCAGGTGG TTAGAGAGAA GGCGACCGGG 
GACGTCTATG CCATGAAAAT CATGAAGAAG AAGGCTTTGC TGGCCCAGGA ACAGGTTTCA 
TTTTTCGAGG AGGAGAGGAA CATATTATCT CGGAGCACGA GTCCTTGGAT CCCCCAGTTA 
CAGTACGCCT TTCAGGACAA AAATAACCTT TACCTGGTCA TGGAATATCA GCCTGGAGGG 
GATTTGCTGT CGCTTCTGAA CAGATACGAG GACCAATTAG ATGAGAGCAT GATCCAGTTT 
TACCTTGCTG AGCTGATTTT GGCTGTCCAC AGCGTGCACC AGATGGGATA TGTGCATCGA 
GACATCAAGC CCGAGAACAT CCTCATCGAC CGGACGGGAC ACATCAAGCT GGTGGATTTT 
GGATCAGCCG CTAAGATGAA TTCAAATAAG GTGGATGCCA AACTCCCCAT TGGGACCCCG 
GATTACATGG CTCCGGAAGT GTTGACCGTG ATGAACGAGG ACCGAAGGGG CACATACGGC 
TTGGACTGTG ACTGGTGGTC TGTCGGAGTT GTTGCTTATG AGATGGTTTA TGGGAAGACC 
CCATTCACAG AGGGAACCTC CGCCCGGACC TTCAACAACA TCATGAACTT CCAGCGGTTT 
TTGAAGTTCC CAGATGACCC CAAAGTTAGC AGTGAGCTCC TTGATCTGCT TCAGAGTCTG 
CTGTGTGTCC AGAAAGAGAG ACTGAAGTTC GAGGGTCTCT GCTGCCACCC TTTCTTTGCC 
AGAACGGACT GGAACAACAT CCGTAACTCT CCTCCCCCCT TCGTCCCCAC CCTCAAGTCT 
GACGATGACA CCTCCAATTT TGATGAACCA GAGAAGAATT CGTGGGTTTC ATCCTCTGTG 
TGCCAGCTGA GCCCCTCGGG CTTCTCAGGC GAAGAGCTGC CGTTTGTGGG ATTTTCGTAC 
AGCAAGGCAC TGGGGTATCT TGGTAGATCT GAGTCTGTCG TGTCGAGTCT GGACTCCCCT 
GCCAAGGTTA GCTCCATGGA AAAGAAACTT CTCATCAAAA GCAAAGAGCT CCAAGACTCC 
CAGGACAAGT GTCACAAGAT GGAGCAGGAA ATGACCCGGT TACATCGCAG AGTGTCAGAG 
GTGGAGGCTG TGCTTAGTCA GAAGGAGGTG GAGCTGAAGG CCTCTGAGAC TCAGAGATCC 
CTCCTGGAGC AGGACCTTGC TACCTACATC ACAGAATGCA GTAGCTTAAA GCGAAGTTTG 
GAGCAAGCGC GGATGGAGGT GTCCCAGGAG GATGACAAAG CTCTGCAGCT TCTCCACGAC 
ATCCGAGAGC AGAGCCGGAA GCTCCAGGAG ATCAAGGAGC AGGAGTACCA GGCTCAGGTG 
GAGGAGATGA GGCTGATGAT GAATCAGCTG GAAGAAGACC TGGTGTCAGC CCGCAGACGC 
AGCGATCTCT ACGAGTCTGA GCTGAGGGAG TCTCGGCTTG CCGCCGAGGA ATTCAAGCGG 
AAGGCAAACG AATGTCAGCA CAAACTGATG AAGGTAGTCA GCCACCCTCC AAGAGGGGAC 
TCCGGGGGCA CGGCCCTGGA TGATCTTCAC AAGATGCAAG GCCACGCTGG CTTAACGAGT 
GCTAAGGACC AAGGGAAGCC TGAAGTGGGA GAATATTCCA AACTGGAGAA GATCAATGCT 
GAGCAGCAGC TGAAGATCCA GGAGCTCCAG GAGAAGCTGG AGAAGGCTGT AAAAGCCAGC 
ACAGAGGCCA CCGAGCTCCT GCAGAATATC CGCCAGGCAA AGGAGCGAGC AGAGCGGGAG 
CTAGAGAAGC TACACAACCG GGAAGACTCC TCCGAGGGCA TCAAAAAGAA GCTGGTGGAA 
GCCGAGGAAC GCCGCCACTC CCTGGAGAAC AAGGTAAAGA GACTAGAGAC CATGGAGCGT 
AGAGAGAACA GACTGAAGGA TGACATCCAG ACAAAGTCCG AACAGATCCA GCAGATGGCT 
GATAAAATTC TGGAGCTGGA GGAGAAACAT CGGGAGGCTC AGGTCTCAGC TCAACATCTA 
GAAGTACACT TGAAACAGAA AGAACAGCAC TACGAGGAAA AGATCAAGGT ATTGGACAAT 
CAGATAAAGA AGGACCTGGC CGACAAGGAG AGCCTGGAGA ACATGATGCA GAGACACGAG 
GAGGAGGCCC ACGAGAAGGG CAAGATCCTC AGCGAGCAGA AGGCGATGAT CAACGCGATG 
GATTCCAAGA TCCGATCCCT GGAGCAGAGG ATCGTGGAGC TGTCGGAAGC CAACAAGCTT 
GCGGCAAACA GCAGTCTCTT CACCCAGAGG AACATGAAGG CCCAGGAAGA GATGATCTCA 
GAACTCAGGC AGCAGAAATT TTACCTGGAG ACGCAGGCCG GAAAGCTGGA GGCCCAGAAC 
CGAAAGCTGG AAGAGCAACT GGAGAAGATC AGCCACCAAG ATCACAGTGA CAAGAGTCGG 
CTGCTGGAGC TGGAAACAAG GCTGAGGGAG GTCAGCCTGG AGCACGAGGA GCAGAAGCTG 
GAGCTGAAGC GGCAGCTCAC GGAGCTGCAG CTGTCCCTGC AGGAGCGCGA GTCCCAGCTG 
ACGGCCCTGC AGGCCGCCCG GGCAGCGCTG GAGAGCCAGC TCCGCCAGGC GAAGACAGAG 
CTGGAGGAGA CAACCGCGGA AGCGGAGGAG GAGATCCAGG CGCTCACGGC ACATCGAGAT 
GAGATCCAGC GCAAATTCGA TGCCCTTCGC AACAGCTGCA CGGTCATCAC CGACCTGGAG 
GAGCAGCTGA ACCAGCTCAC CGAGGACAAC GCCGAGCTCA ACAACCAAAA CTTCTACCTG 
TCCAAACAAC TCGATGAGGC TTCCGGGGCC AATGACGAGA TTGTGCAGCT GCGGAGTGAG 
GTGGACCATC TCCGCCGTGA GATCACGGAG CGGGAGATGC AGCTCACCAG CCAGAAGCAA 
GCACAACTCA GTGCTCCTGA TTTGCAGACG ATGGAGGCTC TGAAGACGAC ATGCACCATG 
CTGGAGGAGC AGGTCCTGGA CCTGGAGGCC CTGAACGACG AGCTGCTGGA GAAGGAGCGC 
CAGTGGGAGG CCTGGCGGAG CGTCCTCGGC GACGAGAAGT CCCAGTTCGA GTGTCGAGTT 
CGAGAGCTAC AGAGGATGCT GGACACCGAG AAGCAGAGCA GGGCTAGGGC CGATCAGCGG 
ATCACCGAGT CGCGCCAGGT GGTGGAGTTG GCGGTGAAGG AACACAAGGC CGAGATTCTT 
GCTCTGCAGC AGGCTCTCAA GGAGCAGAAG CTCAAAGCCG AGAGCCTGTC GGACAAGCTC 
AACGACCTGG AGAAGAAGCA CGCCATGCTG GAGATGAACG CCCGGAGCTT ACAGCAGAAA 
CTAGAGACAG AGCGGGAGCT CAAACAGAGG CTTCTGGAGG AGCAAGCCAA ATTGCAGCAG 
CAGATGGACC TGCAGAAGAA CCACATCTTC AGACTGACGC AAGGGCTGCA GGAGGCGCTG 
GACCGGGCGG ATCTGCTGAA GACAGAAAGG AGCGACCTGG AGTACCAGCT GGAAAACATT 
CAGGTTCTCT ACTCTCACGA GAAAGTGAAA ATGGAAGGCA CAATCTCTCA GCAAACCAAA 
CTCATTGATT TCCTGCAAGC CAAAATGGAC CAGCCTGCTA AAAAGAAAAA GGTTCCTCTG 
CAGTACAATG AGCTGAAGCT AGCCCTGGAA AAGGAGAAAG CCCGATGCGC GGAGCTGGAG 
GAGGCCCTTC AGAAGACCCG CATCGAACTC CGCTCTGCGC GGGAGGAAGC TGCCCACCGC 
AAAGCCACGG ACCACCCGCA CCCGTCTACG CCAGCCACTG CGAGGCAGCA GATCGCCATG 
TCTGCCATTG TGCGGTCGCC CGAGCACCAG CCCAGTGCCA TGAGCCTGCT TGCCCCACCA 
TCCAGCCGCA GAAAGGAGTC GTCAACTCCA GAGGAATTCA GCCGGCGTCT GAAAGAGCGC 
ATGCACCACA ACATCCCTCA CCGGTTTAAT GTGGGCCTGA ACATGAGAGC CACCAAGTGC 
GCCGTGTGTC TGGATACTGT GCACTTTGGA CGCCAGGCAT CCAAATGCCT CGAATGTCAA 
GTGATGTGTC ACCCCAAATG CTCCACCTGC TTGCCCGCTA CCTGTGGCCT GCCAGCTGAA 
TATGCCACAC ACTTCACTGA GGCCTTCTGC CGGGACAAAA TGAACTCCCC GGGGCTCCAG 
AGCAAGGAGC CTGGCAGCAG CTTGCACCTG GAAGGGTGGA TGAAAGTGCC CAGAAATAAC 
AAACGGGGAC AGCAAGGCTG GGACAGGAAG TACATTGTTC TGGAGGGGTC AAAAGTCCTC 
ATCTATGACA ATGAAGCCAG AGAAGCTGGA CAGAGGCCGG TGGAAGAATT TGAGCTGTGC 
CTTCCTGACG GGGACGTATC TATTCATGGT GCCGTTGGTG CTTCAGAACT TGCAAATACC 
GCCAAAGCAG ATGTCCCATA CATCCTGAAG ATGGAGTCTC ACCCACACAC CACCTGCTGG 
CCTGGGAGAA CCCTCTACTT GCTAGCACCC AGCTTCCCCG ACAAGCAGCG CTGGGTCACC 
GCCTTAGAAT CTGTCGTCGC AGGTGGGAGA GTTTCTAGGG AAAAGGCCGA AGCCGATGCT 
AAATTACTTG GAAACTCTCT GCTGAAACTG GAGGGCGATG ACCGGCTTGA CATGAACTGC 
ACCCTGCCCT TCAGTGACCA GGTAGTGCTG GTGGGCACGG AGGAAGGCCT CTACGCCCTG 
AATGTCTTGA AAAACTCCTT AACCCACATC CCAGGGATTG GCGCAGTCTT CCAAATTTAC 
ATCATCAAGG ACCTGGAGAA GCTGCTCATG ATAGCAGGGG AAGAGCGGGC TCTGTGCCTG 
GTGGACGTGA AGAAGGTGAA GCAGTCCCTG GCGCAGTCAC ACCTGCCTGC CCAGCCCGAC 
GTCTCCCCCA ACATATTCGA AGCCGTCAAA GGCTGCCACT TGTTCGCTGC TGGCAAGATC 
GAGAACAGCC TGTGCATCTG CGCCGCTATG CCAAGCAAAG TCGTCATCCT CCGCTACAAT 
GACAACCTCA GCAAGTACTG CATCCGCAAG GAGATCGAGA CCTCAGAGCC CTGCAGCTGT 
ATCCACTTCA CCAACTACAG CATCCTCATT GGGACCAACA AATTCTATGA GATCGACATG 
AAGCAGTACA CGCTTGACGA GTTCCTGGAC AAGAACGACC ATTCCTTGGC ACCTGCTGTG 
TTCGCCTCCT CGTCCAACAG CTTCCCTGTC TCCATTGTGC AGGCGAACAG CGCCGGGCAG 
CGAGAAGAAT ACCTGCTGTG CTTCCACGAA TTTGGGGTGT TCGTGGATTC TTACGGAAGA 
CGTAGCCGCA CAGATGATCT CAAGTGGAGT CGCTTACCTC TGGCCTTCGC CTACAGAGAA 
CCTTATCTGT TTGTGACTCA CTTCAACTCC CTGGAAGTCA TTGAGATCCA GGCCAGATCC 
TCACTGGGGA GCCCTGCCCG AGCATATCTG GAAATTCCAA ACCCTCGCTA CCTGGGCCCC 
GCGATTTCCT CCGGAGCGAT TTACCTGGCC TCCTCATACC AGGACAAGTT AAGGGTCATA 
TGCTGCAAAG GAAACCTCGT GAAGGAGTCA GGCACTGAGC AGCACCGGGT GCCCTCCACC 
TCCCGCAGCA GCCCCAACAA GCGAGGCCCA CCAACATACA ACGAGCACAT CACCAAACGC 
GTGGCCTCCA GCCCGGCGCC ACCGGAAGGC CCCAGCCACC CCCGAGAGCC AAGCACACCG 
CACCGCTACC GAGACAGAGA GGGTCGGACA GAGCTGCGCA GGGACAAGTC TCCAGGCCGC 
CCTCTGGAGC GGGAGAAGTC CCCAGGCCGA ATGCTCAGCA CTAGGAGAGA GCGGTCCCCA 
GGGAGACTGT TTGAAGACAG CAGCAGGGGC CGGCTGCCTG CAGGAGCAGT GAGGACCCCA 
CTGTCCCAGG TTAACAAGGT CTGGGACCAG TCTTCAGTAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030109960.1
CDS	158..6439
Translation	MLKFKYGVRNPPEASASEPIASRASRLNLFFQGKPPLMTQQQMSALSREGMLDALFALFEECSQPALMKMKHVSSFVQKYSDTIAELRELQPSARDFEVRSLVGCGHFAEVQVVREKATGDVYAMKIMKKKALLAQEQVSFFEEERNILSRSTSPWIPQLQYAFQDKNNLYLVMEYQPGGDLLSLLNRYEDQLDESMIQFYLAELILAVHSVHQMGYVHRDIKPENILIDRTGHIKLVDFGSAAKMNSNKVDAKLPIGTPDYMAPEVLTVMNEDRRGTYGLDCDWWSVGVVAYEMVYGKTPFTEGTSARTFNNIMNFQRFLKFPDDPKVSSELLDLLQSLLCVQKERLKFEGLCCHPFFARTDWNNIRNSPPPFVPTLKSDDDTSNFDEPEKNSWVSSSVCQLSPSGFSGEELPFVGFSYSKALGYLGRSESVVSSLDSPAKVSSMEKKLLIKSKELQDSQDKCHKMEQEMTRLHRRVSEVEAVLSQKEVELKASETQRSLLEQDLATYITECSSLKRSLEQARMEVSQEDDKALQLLHDIREQSRKLQEIKEQEYQAQVEEMRLMMNQLEEDLVSARRRSDLYESELRESRLAAEEFKRKANECQHKLMKVVSHPPRGDSGGTALDDLHKMQGHAGLTSAKDQGKPEVGEYSKLEKINAEQQLKIQELQEKLEKAVKASTEATELLQNIRQAKERAERELEKLHNREDSSEGIKKKLVEAEERRHSLENKVKRLETMERRENRLKDDIQTKSEQIQQMADKILELEEKHREAQVSAQHLEVHLKQKEQHYEEKIKVLDNQIKKDLADKESLENMMQRHEEEAHEKGKILSEQKAMINAMDSKIRSLEQRIVELSEANKLAANSSLFTQRNMKAQEEMISELRQQKFYLETQAGKLEAQNRKLEEQLEKISHQDHSDKSRLLELETRLREVSLEHEEQKLELKRQLTELQLSLQERESQLTALQAARAALESQLRQAKTELEETTAEAEEEIQALTAHRDEIQRKFDALRNSCTVITDLEEQLNQLTEDNAELNNQNFYLSKQLDEASGANDEIVQLRSEVDHLRREITEREMQLTSQKQAQLSAPDLQTMEALKTTCTMLEEQVLDLEALNDELLEKERQWEAWRSVLGDEKSQFECRVRELQRMLDTEKQSRARADQRITESRQVVELAVKEHKAEILALQQALKEQKLKAESLSDKLNDLEKKHAMLEMNARSLQQKLETERELKQRLLEEQAKLQQQMDLQKNHIFRLTQGLQEALDRADLLKTERSDLEYQLENIQVLYSHEKVKMEGTISQQTKLIDFLQAKMDQPAKKKKVPLQYNELKLALEKEKARCAELEEALQKTRIELRSAREEAAHRKATDHPHPSTPATARQQIAMSAIVRSPEHQPSAMSLLAPPSSRRKESSTPEEFSRRLKERMHHNIPHRFNVGLNMRATKCAVCLDTVHFGRQASKCLECQVMCHPKCSTCLPATCGLPAEYATHFTEAFCRDKMNSPGLQSKEPGSSLHLEGWMKVPRNNKRGQQGWDRKYIVLEGSKVLIYDNEAREAGQRPVEEFELCLPDGDVSIHGAVGASELANTAKADVPYILKMESHPHTTCWPGRTLYLLAPSFPDKQRWVTALESVVAGGRVSREKAEADAKLLGNSLLKLEGDDRLDMNCTLPFSDQVVLVGTEEGLYALNVLKNSLTHIPGIGAVFQIYIIKDLEKLLMIAGEERALCLVDVKKVKQSLAQSHLPAQPDVSPNIFEAVKGCHLFAAGKIENSLCICAAMPSKVVILRYNDNLSKYCIRKEIETSEPCSCIHFTNYSILIGTNKFYEIDMKQYTLDEFLDKNDHSLAPAVFASSSNSFPVSIVQANSAGQREEYLLCFHEFGVFVDSYGRRSRTDDLKWSRLPLAFAYREPYLFVTHFNSLEVIEIQARSSLGSPARAYLEIPNPRYLGPAISSGAIYLASSYQDKLRVICCKGNLVKESGTEQHRVPSTSRSSPNKRGPPTYNEHITKRVASSPAPPEGPSHPREPSTPHRYRDREGRTELRRDKSPGRPLEREKSPGRMLSTRRERSPGRLFEDSSRGRLPAGAVRTPLSQVNKVWDQSSV

Target ORF information:

RefSeq Version	XM_030254100.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus citron (Cit), transcript variant X12, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030254100.1

ORF Insert Sequence:

ATGTTGAAGT TCAAGTATGG TGTGCGGAAC CCGCCGGAGG CCAGTGCCTC CGAGCCCATT 
GCCAGTCGGG CCTCCAGGCT AAATCTCTTC TTCCAGGGGA AACCGCCCCT CATGACTCAA 
CAGCAGATGT CTGCTCTTTC CCGGGAAGGG ATGCTAGACG CCCTCTTCGC TCTCTTTGAA 
GAGTGCAGCC AACCCGCCCT GATGAAGATG AAGCACGTGA GCAGCTTTGT CCAGAAGTAT 
TCCGACACCA TAGCCGAGTT GCGGGAGCTG CAGCCGTCGG CGAGAGACTT CGAAGTTCGA 
AGCCTTGTGG GCTGTGGTCA CTTCGCTGAA GTGCAGGTGG TTAGAGAGAA GGCGACCGGG 
GACGTCTATG CCATGAAAAT CATGAAGAAG AAGGCTTTGC TGGCCCAGGA ACAGGTTTCA 
TTTTTCGAGG AGGAGAGGAA CATATTATCT CGGAGCACGA GTCCTTGGAT CCCCCAGTTA 
CAGTACGCCT TTCAGGACAA AAATAACCTT TACCTGGTCA TGGAATATCA GCCTGGAGGG 
GATTTGCTGT CGCTTCTGAA CAGATACGAG GACCAATTAG ATGAGAGCAT GATCCAGTTT 
TACCTTGCTG AGCTGATTTT GGCTGTCCAC AGCGTGCACC AGATGGGATA TGTGCATCGA 
GACATCAAGC CCGAGAACAT CCTCATCGAC CGGACGGGAC ACATCAAGCT GGTGGATTTT 
GGATCAGCCG CTAAGATGAA TTCAAATAAG GTGGATGCCA AACTCCCCAT TGGGACCCCG 
GATTACATGG CTCCGGAAGT GTTGACCGTG ATGAACGAGG ACCGAAGGGG CACATACGGC 
TTGGACTGTG ACTGGTGGTC TGTCGGAGTT GTTGCTTATG AGATGGTTTA TGGGAAGACC 
CCATTCACAG AGGGAACCTC CGCCCGGACC TTCAACAACA TCATGAACTT CCAGCGGTTT 
TTGAAGTTCC CAGATGACCC CAAAGTTAGC AGTGAGCTCC TTGATCTGCT TCAGAGTCTG 
CTGTGTGTCC AGAAAGAGAG ACTGAAGTTC GAGGGTCTCT GCTGCCACCC TTTCTTTGCC 
AGAACGGACT GGAACAACAT CCGTAACTCT CCTCCCCCCT TCGTCCCCAC CCTCAAGTCT 
GACGATGACA CCTCCAATTT TGATGAACCA GAGAAGAATT CGTGGGTTTC ATCCTCTGTG 
TGCCAGCTGA GCCCCTCGGG CTTCTCAGGC GAAGAGCTGC CGTTTGTGGG ATTTTCGTAC 
AGCAAGGCAC TGGGGTATCT TGGTAGATCT GAGTCTGTCG TGTCGAGTCT GGACTCCCCT 
GCCAAGGTTA GCTCCATGGA AAAGAAACTT CTCATCAAAA GCAAAGAGCT CCAAGACTCC 
CAGGACAAGT GTCACAAGAT GGAGCAGGAA ATGACCCGGT TACATCGCAG AGTGTCAGAG 
GTGGAGGCTG TGCTTAGTCA GAAGGAGGTG GAGCTGAAGG CCTCTGAGAC TCAGAGATCC 
CTCCTGGAGC AGGACCTTGC TACCTACATC ACAGAATGCA GTAGCTTAAA GCGAAGTTTG 
GAGCAAGCGC GGATGGAGGT GTCCCAGGAG GATGACAAAG CTCTGCAGCT TCTCCACGAC 
ATCCGAGAGC AGAGCCGGAA GCTCCAGGAG ATCAAGGAGC AGGAGTACCA GGCTCAGGTG 
GAGGAGATGA GGCTGATGAT GAATCAGCTG GAAGAAGACC TGGTGTCAGC CCGCAGACGC 
AGCGATCTCT ACGAGTCTGA GCTGAGGGAG TCTCGGCTTG CCGCCGAGGA ATTCAAGCGG 
AAGGCAAACG AATGTCAGCA CAAACTGATG AAGGTAGTCA GCCACCCTCC AAGAGGGGAC 
TCCGGGGGCA CGGCCCTGGA TGATCTTCAC AAGATGCAAG GCCACGCTGG CTTAACGAGT 
GCTAAGGACC AAGGGAAGCC TGAAGTGGGA GAATATTCCA AACTGGAGAA GATCAATGCT 
GAGCAGCAGC TGAAGATCCA GGAGCTCCAG GAGAAGCTGG AGAAGGCTGT AAAAGCCAGC 
ACAGAGGCCA CCGAGCTCCT GCAGAATATC CGCCAGGCAA AGGAGCGAGC AGAGCGGGAG 
CTAGAGAAGC TACACAACCG GGAAGACTCC TCCGAGGGCA TCAAAAAGAA GCTGGTGGAA 
GCCGAGGAAC GCCGCCACTC CCTGGAGAAC AAGGTAAAGA GACTAGAGAC CATGGAGCGT 
AGAGAGAACA GACTGAAGGA TGACATCCAG ACAAAGTCCG AACAGATCCA GCAGATGGCT 
GATAAAATTC TGGAGCTGGA GGAGAAACAT CGGGAGGCTC AGGTCTCAGC TCAACATCTA 
GAAGTACACT TGAAACAGAA AGAACAGCAC TACGAGGAAA AGATCAAGGT ATTGGACAAT 
CAGATAAAGA AGGACCTGGC CGACAAGGAG AGCCTGGAGA ACATGATGCA GAGACACGAG 
GAGGAGGCCC ACGAGAAGGG CAAGATCCTC AGCGAGCAGA AGGCGATGAT CAACGCGATG 
GATTCCAAGA TCCGATCCCT GGAGCAGAGG ATCGTGGAGC TGTCGGAAGC CAACAAGCTT 
GCGGCAAACA GCAGTCTCTT CACCCAGAGG AACATGAAGG CCCAGGAAGA GATGATCTCA 
GAACTCAGGC AGCAGAAATT TTACCTGGAG ACGCAGGCCG GAAAGCTGGA GGCCCAGAAC 
CGAAAGCTGG AAGAGCAACT GGAGAAGATC AGCCACCAAG ATCACAGTGA CAAGAGTCGG 
CTGCTGGAGC TGGAAACAAG GCTGAGGGAG GTCAGCCTGG AGCACGAGGA GCAGAAGCTG 
GAGCTGAAGC GGCAGCTCAC GGAGCTGCAG CTGTCCCTGC AGGAGCGCGA GTCCCAGCTG 
ACGGCCCTGC AGGCCGCCCG GGCAGCGCTG GAGAGCCAGC TCCGCCAGGC GAAGACAGAG 
CTGGAGGAGA CAACCGCGGA AGCGGAGGAG GAGATCCAGG CGCTCACGGC ACATCGAGAT 
GAGATCCAGC GCAAATTCGA TGCCCTTCGC AACAGCTGCA CGGTCATCAC CGACCTGGAG 
GAGCAGCTGA ACCAGCTCAC CGAGGACAAC GCCGAGCTCA ACAACCAAAA CTTCTACCTG 
TCCAAACAAC TCGATGAGGC TTCCGGGGCC AATGACGAGA TTGTGCAGCT GCGGAGTGAG 
GTGGACCATC TCCGCCGTGA GATCACGGAG CGGGAGATGC AGCTCACCAG CCAGAAGCAA 
GCACAACTCA GTGCTCCTGA TTTGCAGACG ATGGAGGCTC TGAAGACGAC ATGCACCATG 
CTGGAGGAGC AGGTCCTGGA CCTGGAGGCC CTGAACGACG AGCTGCTGGA GAAGGAGCGC 
CAGTGGGAGG CCTGGCGGAG CGTCCTCGGC GACGAGAAGT CCCAGTTCGA GTGTCGAGTT 
CGAGAGCTAC AGAGGATGCT GGACACCGAG AAGCAGAGCA GGGCTAGGGC CGATCAGCGG 
ATCACCGAGT CGCGCCAGGT GGTGGAGTTG GCGGTGAAGG AACACAAGGC CGAGATTCTT 
GCTCTGCAGC AGGCTCTCAA GGAGCAGAAG CTCAAAGCCG AGAGCCTGTC GGACAAGCTC 
AACGACCTGG AGAAGAAGCA CGCCATGCTG GAGATGAACG CCCGGAGCTT ACAGCAGAAA 
CTAGAGACAG AGCGGGAGCT CAAACAGAGG CTTCTGGAGG AGCAAGCCAA ATTGCAGCAG 
CAGATGGACC TGCAGAAGAA CCACATCTTC AGACTGACGC AAGGGCTGCA GGAGGCGCTG 
GACCGGGCGG ATCTGCTGAA GACAGAAAGG AGCGACCTGG AGTACCAGCT GGAAAACATT 
CAGGTTCTCT ACTCTCACGA GAAAGTGAAA ATGGAAGGCA CAATCTCTCA GCAAACCAAA 
CTCATTGATT TCCTGCAAGC CAAAATGGAC CAGCCTGCTA AAAAGAAAAA GGTTCCTCTG 
CAGTACAATG AGCTGAAGCT AGCCCTGGAA AAGGAGAAAG CCCGATGCGC GGAGCTGGAG 
GAGGCCCTTC AGAAGACCCG CATCGAACTC CGCTCTGCGC GGGAGGAAGC TGCCCACCGC 
AAAGCCACGG ACCACCCGCA CCCGTCTACG CCAGCCACTG CGAGGCAGCA GATCGCCATG 
TCTGCCATTG TGCGGTCGCC CGAGCACCAG CCCAGTGCCA TGAGCCTGCT TGCCCCACCA 
TCCAGCCGCA GAAAGGAGTC GTCAACTCCA GAGGAATTCA GCCGGCGTCT GAAAGAGCGC 
ATGCACCACA ACATCCCTCA CCGGTTTAAT GTGGGCCTGA ACATGAGAGC CACCAAGTGC 
GCCGTGTGTC TGGATACTGT GCACTTTGGA CGCCAGGCAT CCAAATGCCT CGAATGTCAA 
GTGATGTGTC ACCCCAAATG CTCCACCTGC TTGCCCGCTA CCTGTGGCCT GCCAGCTGAA 
TATGCCACAC ACTTCACTGA GGCCTTCTGC CGGGACAAAA TGAACTCCCC GGGGCTCCAG 
AGCAAGGAGC CTGGCAGCAG CTTGCACCTG GAAGGGTGGA TGAAAGTGCC CAGAAATAAC 
AAACGGGGAC AGCAAGGCTG GGACAGGAAG TACATTGTTC TGGAGGGGTC AAAAGTCCTC 
ATCTATGACA ATGAAGCCAG AGAAGCTGGA CAGAGGCCGG TGGAAGAATT TGAGCTGTGC 
CTTCCTGACG GGGACGTATC TATTCATGGT GCCGTTGGTG CTTCAGAACT TGCAAATACC 
GCCAAAGCAG ATGTCCCATA CATCCTGAAG ATGGAGTCTC ACCCACACAC CACCTGCTGG 
CCTGGGAGAA CCCTCTACTT GCTAGCACCC AGCTTCCCCG ACAAGCAGCG CTGGGTCACC 
GCCTTAGAAT CTGTCGTCGC AGGTGGGAGA GTTTCTAGGG AAAAGGCCGA AGCCGATGCT 
AAATTACTTG GAAACTCTCT GCTGAAACTG GAGGGCGATG ACCGGCTTGA CATGAACTGC 
ACCCTGCCCT TCAGTGACCA GGTAGTGCTG GTGGGCACGG AGGAAGGCCT CTACGCCCTG 
AATGTCTTGA AAAACTCCTT AACCCACATC CCAGGGATTG GCGCAGTCTT CCAAATTTAC 
ATCATCAAGG ACCTGGAGAA GCTGCTCATG ATAGCAGGGG AAGAGCGGGC TCTGTGCCTG 
GTGGACGTGA AGAAGGTGAA GCAGTCCCTG GCGCAGTCAC ACCTGCCTGC CCAGCCCGAC 
GTCTCCCCCA ACATATTCGA AGCCGTCAAA GGCTGCCACT TGTTCGCTGC TGGCAAGATC 
GAGAACAGCC TGTGCATCTG CGCCGCTATG CCAAGCAAAG TCGTCATCCT CCGCTACAAT 
GACAACCTCA GCAAGTACTG CATCCGCAAG GAGATCGAGA CCTCAGAGCC CTGCAGCTGT 
ATCCACTTCA CCAACTACAG CATCCTCATT GGGACCAACA AATTCTATGA GATCGACATG 
AAGCAGTACA CGCTTGACGA GTTCCTGGAC AAGAACGACC ATTCCTTGGC ACCTGCTGTG 
TTCGCCTCCT CGTCCAACAG CTTCCCTGTC TCCATTGTGC AGGCGAACAG CGCCGGGCAG 
CGAGAAGAAT ACCTGCTGTG CTTCCACGAA TTTGGGGTGT TCGTGGATTC TTACGGAAGA 
CGTAGCCGCA CAGATGATCT CAAGTGGAGT CGCTTACCTC TGGCCTTCGC CTACAGAGAA 
CCTTATCTGT TTGTGACTCA CTTCAACTCC CTGGAAGTCA TTGAGATCCA GGCCAGATCC 
TCACTGGGGA GCCCTGCCCG AGCATATCTG GAAATTCCAA ACCCTCGCTA CCTGGGCCCC 
GCGATTTCCT CCGGAGCGAT TTACCTGGCC TCCTCATACC AGGACAAGTT AAGGGTCATA 
TGCTGCAAAG GAAACCTCGT GAAGGAGTCA GGCACTGAGC AGCACCGGGT GCCCTCCACC 
TCCCGCAGCA GCCCCAACAA GCGAGGCCCA CCAACATACA ACGAGCACAT CACCAAACGC 
GTGGCCTCCA GCCCGGCGCC ACCGGAAGGC CCCAGCCACC CCCGAGAGCC AAGCACACCG 
CACCGCTACC GAGACAGAGA GGGTCGGACA GAGCTGCGCA GGGACAAGTC TCCAGGCCGC 
CCTCTGGAGC GGGAGAAGTC CCCAGGCCGA ATGCTCAGCA CTAGGAGAGA GCGGTCCCCA 
GGGAGACTGT TTGAAGACAG CAGCAGGGGC CGGCTGCCTG CAGGAGCAGT GAGGACCCCA 
CTGTCCCAGG TTAACAAGGT CTGGGACCAG TCTTCAGTAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cit ( NM_007708.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_031734.3 Mus musculus citron (Cit), mRNA.

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CloneID	OMu00646
Clone ID Related Accession (Same CDS sequence)	XM_006530141.2 , XM_006530141.4 , NM_007708.3 , XM_006530141.3
Accession Version	NM_007708.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6168bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-25
Organism	Mus musculus(house mouse)
Product	citron Rho-interacting kinase
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC162461.6 and AC115795.11. On Feb 6, 2007 this sequence version replaced NM_007708.2. Sequence Note: This sequence has been modified as follows: removed 39 bp suspected to be vector contamination from the 5' end. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMN00849384, SAMN00849385 [ECO:0000348] ##Evidence-Data-END##

ATGTTGAAGT TCAAGTATGG TGTGCGGAAC CCGCCGGAGG CCAGTGCCTC CGAGCCCATT 
GCCAGTCGGG CCTCCAGGCT AAATCTCTTC TTCCAGGGGA AACCGCCCCT CATGACTCAA 
CAGCAGATGT CTGCTCTTTC CCGGGAAGGG ATGCTAGACG CCCTCTTCGC TCTCTTTGAA 
GAGTGCAGCC AACCCGCCCT GATGAAGATG AAGCACGTGA GCAGCTTTGT CCAGAAGTAT 
TCCGACACCA TAGCCGAGTT GCGGGAGCTG CAGCCGTCGG CGAGAGACTT CGAAGTTCGA 
AGCCTTGTGG GCTGTGGTCA CTTCGCTGAA GTGCAGGTGG TTAGAGAGAA GGCGACCGGG 
GACGTCTATG CCATGAAAAT CATGAAGAAG AAGGCTTTGC TGGCCCAGGA ACAGGTTTCA 
TTTTTCGAGG AGGAGAGGAA CATATTATCT CGGAGCACGA GTCCTTGGAT CCCCCAGTTA 
CAGTACGCCT TTCAGGACAA AAATAACCTT TACCTGGTCA TGGAATATCA GCCTGGAGGG 
GATTTGCTGT CGCTTCTGAA CAGATACGAG GACCAATTAG ATGAGAGCAT GATCCAGTTT 
TACCTTGCTG AGCTGATTTT GGCTGTCCAC AGCGTGCACC AGATGGGATA TGTGCATCGA 
GACATCAAGC CCGAGAACAT CCTCATCGAC CGGACGGGAC ACATCAAGCT GGTGGATTTT 
GGATCAGCCG CTAAGATGAA TTCAAATAAG GTGGATGCCA AACTCCCCAT TGGGACCCCG 
GATTACATGG CTCCGGAAGT GTTGACCGTG ATGAACGAGG ACCGAAGGGG CACATACGGC 
TTGGACTGTG ACTGGTGGTC TGTCGGAGTT GTTGCTTATG AGATGGTTTA TGGGAAGACC 
CCATTCACAG AGGGAACCTC CGCCCGGACC TTCAACAACA TCATGAACTT CCAGCGGTTT 
TTGAAGTTCC CAGATGACCC CAAAGTTAGC AGTGAGCTCC TTGATCTGCT TCAGAGTCTG 
CTGTGTGTCC AGAAAGAGAG ACTGAAGTTC GAGGGTCTCT GCTGCCACCC TTTCTTTGCC 
AGAACGGACT GGAACAACAT CCGTAACTCT CCTCCCCCCT TCGTCCCCAC CCTCAAGTCT 
GACGATGACA CCTCCAATTT TGATGAACCA GAGAAGAATT CGTGGGTTTC ATCCTCTGTG 
TGCCAGCTGA GCCCCTCGGG CTTCTCAGGC GAAGAGCTGC CGTTTGTGGG ATTTTCGTAC 
AGCAAGGCAC TGGGGTATCT TGGTAGATCT GAGTCTGTCG TGTCGAGTCT GGACTCCCCT 
GCCAAGGTTA GCTCCATGGA AAAGAAACTT CTCATCAAAA GCAAAGAGCT CCAAGACTCC 
CAGGACAAGT GTCACAAGAT GGAGCAGGAA ATGACCCGGT TACATCGCAG AGTGTCAGAG 
GTGGAGGCTG TGCTTAGTCA GAAGGAGGTG GAGCTGAAGG CCTCTGAGAC TCAGAGATCC 
CTCCTGGAGC AGGACCTTGC TACCTACATC ACAGAATGCA GTAGCTTAAA GCGAAGTTTG 
GAGCAAGCGC GGATGGAGGT GTCCCAGGAG GATGACAAAG CTCTGCAGCT TCTCCACGAC 
ATCCGAGAGC AGAGCCGGAA GCTCCAGGAG ATCAAGGAGC AGGAGTACCA GGCTCAGGTG 
GAGGAGATGA GGCTGATGAT GAATCAGCTG GAAGAAGACC TGGTGTCAGC CCGCAGACGC 
AGCGATCTCT ACGAGTCTGA GCTGAGGGAG TCTCGGCTTG CCGCCGAGGA ATTCAAGCGG 
AAGGCAAACG AATGTCAGCA CAAACTGATG AAGGCTAAGG ACCAAGGGAA GCCTGAAGTG 
GGAGAATATT CCAAACTGGA GAAGATCAAT GCTGAGCAGC AGCTGAAGAT CCAGGAGCTC 
CAGGAGAAGC TGGAGAAGGC TGTAAAAGCC AGCACAGAGG CCACCGAGCT CCTGCAGAAT 
ATCCGCCAGG CAAAGGAGCG AGCAGAGCGG GAGCTAGAGA AGCTACACAA CCGGGAAGAC 
TCCTCCGAGG GCATCAAAAA GAAGCTGGTG GAAGCCGAGG AACGCCGCCA CTCCCTGGAG 
AACAAGGTAA AGAGACTAGA GACCATGGAG CGTAGAGAGA ACAGACTGAA GGATGACATC 
CAGACAAAGT CCGAACAGAT CCAGCAGATG GCTGATAAAA TTCTGGAGCT GGAGGAGAAA 
CATCGGGAGG CTCAGGTCTC AGCTCAACAT CTAGAAGTAC ACTTGAAACA GAAAGAACAG 
CACTACGAGG AAAAGATCAA GGTATTGGAC AATCAGATAA AGAAGGACCT GGCCGACAAG 
GAGAGCCTGG AGAACATGAT GCAGAGACAC GAGGAGGAGG CCCACGAGAA GGGCAAGATC 
CTCAGCGAGC AGAAGGCGAT GATCAACGCG ATGGATTCCA AGATCCGATC CCTGGAGCAG 
AGGATCGTGG AGCTGTCGGA AGCCAACAAG CTTGCGGCAA ACAGCAGTCT CTTCACCCAG 
AGGAACATGA AGGCCCAGGA AGAGATGATC TCAGAACTCA GGCAGCAGAA ATTTTACCTG 
GAGACGCAGG CCGGAAAGCT GGAGGCCCAG AACCGAAAGC TGGAAGAGCA ACTGGAGAAG 
ATCAGCCACC AAGATCACAG TGACAAGAGT CGGCTGCTGG AGCTGGAAAC AAGGCTGAGG 
GAGGTCAGCC TGGAGCACGA GGAGCAGAAG CTGGAGCTGA AGCGGCAGCT CACGGAGCTG 
CAGCTGTCCC TGCAGGAGCG CGAGTCCCAG CTGACGGCCC TGCAGGCCGC CCGGGCAGCG 
CTGGAGAGCC AGCTCCGCCA GGCGAAGACA GAGCTGGAGG AGACAACCGC GGAAGCGGAG 
GAGGAGATCC AGGCGCTCAC GGCACATCGA GATGAGATCC AGCGCAAATT CGATGCCCTT 
CGCAACAGCT GCACGGTCAT CACCGACCTG GAGGAGCAGC TGAACCAGCT CACCGAGGAC 
AACGCCGAGC TCAACAACCA AAACTTCTAC CTGTCCAAAC AACTCGATGA GGCTTCCGGG 
GCCAATGACG AGATTGTGCA GCTGCGGAGT GAGGTGGACC ATCTCCGCCG TGAGATCACG 
GAGCGGGAGA TGCAGCTCAC CAGCCAGAAG CAAACGATGG AGGCTCTGAA GACGACATGC 
ACCATGCTGG AGGAGCAGGT CCTGGACCTG GAGGCCCTGA ACGACGAGCT GCTGGAGAAG 
GAGCGCCAGT GGGAGGCCTG GCGGAGCGTC CTCGGCGACG AGAAGTCCCA GTTCGAGTGT 
CGAGTTCGAG AGCTACAGAG GATGCTGGAC ACCGAGAAGC AGAGCAGGGC TAGGGCCGAT 
CAGCGGATCA CCGAGTCGCG CCAGGTGGTG GAGTTGGCGG TGAAGGAACA CAAGGCCGAG 
ATTCTTGCTC TGCAGCAGGC TCTCAAGGAG CAGAAGCTCA AAGCCGAGAG CCTGTCGGAC 
AAGCTCAACG ACCTGGAGAA GAAGCACGCC ATGCTGGAGA TGAACGCCCG GAGCTTACAG 
CAGAAACTAG AGACAGAGCG GGAGCTCAAA CAGAGGCTTC TGGAGGAGCA AGCCAAATTG 
CAGCAGCAGA TGGACCTGCA GAAGAACCAC ATCTTCAGAC TGACGCAAGG GCTGCAGGAG 
GCGCTGGACC GGGCGGATCT GCTGAAGACA GAAAGGAGCG ACCTGGAGTA CCAGCTGGAA 
AACATTCAGG TTCTCTACTC TCACGAGAAA GTGAAAATGG AAGGCACAAT CTCTCAGCAA 
ACCAAACTCA TTGATTTCCT GCAAGCCAAA ATGGACCAGC CTGCTAAAAA GAAAAAGGTT 
CCTCTGCAGT ACAATGAGCT GAAGCTAGCC CTGGAAAAGG AGAAAGCCCG ATGCGCGGAG 
CTGGAGGAGG CCCTTCAGAA GACCCGCATC GAACTCCGCT CTGCGCGGGA GGAAGCTGCC 
CACCGCAAAG CCACGGACCA CCCGCACCCG TCTACGCCAG CCACTGCGAG GCAGCAGATC 
GCCATGTCTG CCATTGTGCG GTCGCCCGAG CACCAGCCCA GTGCCATGAG CCTGCTTGCC 
CCACCATCCA GCCGCAGAAA GGAGTCGTCA ACTCCAGAGG AATTCAGCCG GCGTCTGAAA 
GAGCGCATGC ACCACAACAT CCCTCACCGG TTTAATGTGG GCCTGAACAT GAGAGCCACC 
AAGTGCGCCG TGTGTCTGGA TACTGTGCAC TTTGGACGCC AGGCATCCAA ATGCCTCGAA 
TGTCAAGTGA TGTGTCACCC CAAATGCTCC ACCTGCTTGC CCGCTACCTG TGGCCTGCCA 
GCTGAATATG CCACACACTT CACTGAGGCC TTCTGCCGGG ACAAAATGAA CTCCCCGGGG 
CTCCAGAGCA AGGAGCCTGG CAGCAGCTTG CACCTGGAAG GGTGGATGAA AGTGCCCAGA 
AATAACAAAC GGGGACAGCA AGGCTGGGAC AGGAAGTACA TTGTTCTGGA GGGGTCAAAA 
GTCCTCATCT ATGACAATGA AGCCAGAGAA GCTGGACAGA GGCCGGTGGA AGAATTTGAG 
CTGTGCCTTC CTGACGGGGA CGTATCTATT CATGGTGCCG TTGGTGCTTC AGAACTTGCA 
AATACCGCCA AAGCAGATGT CCCATACATC CTGAAGATGG AGTCTCACCC ACACACCACC 
TGCTGGCCTG GGAGAACCCT CTACTTGCTA GCACCCAGCT TCCCCGACAA GCAGCGCTGG 
GTCACCGCCT TAGAATCTGT CGTCGCAGGT GGGAGAGTTT CTAGGGAAAA GGCCGAAGCC 
GATGCTAAAT TACTTGGAAA CTCTCTGCTG AAACTGGAGG GCGATGACCG GCTTGACATG 
AACTGCACCC TGCCCTTCAG TGACCAGGTA GTGCTGGTGG GCACGGAGGA AGGCCTCTAC 
GCCCTGAATG TCTTGAAAAA CTCCTTAACC CACATCCCAG GGATTGGCGC AGTCTTCCAA 
ATTTACATCA TCAAGGACCT GGAGAAGCTG CTCATGATAG CAGGGGAAGA GCGGGCTCTG 
TGCCTGGTGG ACGTGAAGAA GGTGAAGCAG TCCCTGGCGC AGTCACACCT GCCTGCCCAG 
CCCGACGTCT CCCCCAACAT ATTCGAAGCC GTCAAAGGCT GCCACTTGTT CGCTGCTGGC 
AAGATCGAGA ACAGCCTGTG CATCTGCGCC GCTATGCCAA GCAAAGTCGT CATCCTCCGC 
TACAATGACA ACCTCAGCAA GTACTGCATC CGCAAGGAGA TCGAGACCTC AGAGCCCTGC 
AGCTGTATCC ACTTCACCAA CTACAGCATC CTCATTGGGA CCAACAAATT CTATGAGATC 
GACATGAAGC AGTACACGCT TGACGAGTTC CTGGACAAGA ACGACCATTC CTTGGCACCT 
GCTGTGTTCG CCTCCTCGTC CAACAGCTTC CCTGTCTCCA TTGTGCAGGC GAACAGCGCC 
GGGCAGCGAG AAGAATACCT GCTGTGCTTC CACGAATTTG GGGTGTTCGT GGATTCTTAC 
GGAAGACGTA GCCGCACAGA TGATCTCAAG TGGAGTCGCT TACCTCTGGC CTTCGCCTAC 
AGAGAACCTT ATCTGTTTGT GACTCACTTC AACTCCCTGG AAGTCATTGA GATCCAGGCC 
AGATCCTCAC TGGGGAGCCC TGCCCGAGCA TATCTGGAAA TTCCAAACCC TCGCTACCTG 
GGCCCCGCGA TTTCCTCCGG AGCGATTTAC CTGGCCTCCT CATACCAGGA CAAGTTAAGG 
GTCATATGCT GCAAAGGAAA CCTCGTGAAG GAGTCAGGCA CTGAGCAGCA CCGGGTGCCC 
TCCACCTCCC GCAGCAGCCC CAACAAGCGA GGCCCACCAA CATACAACGA GCACATCACC 
AAACGCGTGG CCTCCAGCCC GGCGCCACCG GAAGGCCCCA GCCACCCCCG AGAGCCAAGC 
ACACCGCACC GCTACCGAGA CAGAGAGGGT CGGACAGAGC TGCGCAGGGA CAAGTCTCCA 
GGCCGCCCTC TGGAGCGGGA GAAGTCCCCA GGCCGAATGC TCAGCACTAG GAGAGAGCGG 
TCCCCAGGGA GACTGTTTGA AGACAGCAGC AGGGGCCGGC TGCCTGCAGG AGCAGTGAGG 
ACCCCACTGT CCCAGGTTAA CAAGGTCTGG GACCAGTCTT CAGTATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_031734.3
CDS	716..6883
Translation	MLKFKYGVRNPPEASASEPIASRASRLNLFFQGKPPLMTQQQMSALSREGMLDALFALFEECSQPALMKMKHVSSFVQKYSDTIAELRELQPSARDFEVRSLVGCGHFAEVQVVREKATGDVYAMKIMKKKALLAQEQVSFFEEERNILSRSTSPWIPQLQYAFQDKNNLYLVMEYQPGGDLLSLLNRYEDQLDESMIQFYLAELILAVHSVHQMGYVHRDIKPENILIDRTGHIKLVDFGSAAKMNSNKVDAKLPIGTPDYMAPEVLTVMNEDRRGTYGLDCDWWSVGVVAYEMVYGKTPFTEGTSARTFNNIMNFQRFLKFPDDPKVSSELLDLLQSLLCVQKERLKFEGLCCHPFFARTDWNNIRNSPPPFVPTLKSDDDTSNFDEPEKNSWVSSSVCQLSPSGFSGEELPFVGFSYSKALGYLGRSESVVSSLDSPAKVSSMEKKLLIKSKELQDSQDKCHKMEQEMTRLHRRVSEVEAVLSQKEVELKASETQRSLLEQDLATYITECSSLKRSLEQARMEVSQEDDKALQLLHDIREQSRKLQEIKEQEYQAQVEEMRLMMNQLEEDLVSARRRSDLYESELRESRLAAEEFKRKANECQHKLMKAKDQGKPEVGEYSKLEKINAEQQLKIQELQEKLEKAVKASTEATELLQNIRQAKERAERELEKLHNREDSSEGIKKKLVEAEERRHSLENKVKRLETMERRENRLKDDIQTKSEQIQQMADKILELEEKHREAQVSAQHLEVHLKQKEQHYEEKIKVLDNQIKKDLADKESLENMMQRHEEEAHEKGKILSEQKAMINAMDSKIRSLEQRIVELSEANKLAANSSLFTQRNMKAQEEMISELRQQKFYLETQAGKLEAQNRKLEEQLEKISHQDHSDKSRLLELETRLREVSLEHEEQKLELKRQLTELQLSLQERESQLTALQAARAALESQLRQAKTELEETTAEAEEEIQALTAHRDEIQRKFDALRNSCTVITDLEEQLNQLTEDNAELNNQNFYLSKQLDEASGANDEIVQLRSEVDHLRREITEREMQLTSQKQTMEALKTTCTMLEEQVLDLEALNDELLEKERQWEAWRSVLGDEKSQFECRVRELQRMLDTEKQSRARADQRITESRQVVELAVKEHKAEILALQQALKEQKLKAESLSDKLNDLEKKHAMLEMNARSLQQKLETERELKQRLLEEQAKLQQQMDLQKNHIFRLTQGLQEALDRADLLKTERSDLEYQLENIQVLYSHEKVKMEGTISQQTKLIDFLQAKMDQPAKKKKVPLQYNELKLALEKEKARCAELEEALQKTRIELRSAREEAAHRKATDHPHPSTPATARQQIAMSAIVRSPEHQPSAMSLLAPPSSRRKESSTPEEFSRRLKERMHHNIPHRFNVGLNMRATKCAVCLDTVHFGRQASKCLECQVMCHPKCSTCLPATCGLPAEYATHFTEAFCRDKMNSPGLQSKEPGSSLHLEGWMKVPRNNKRGQQGWDRKYIVLEGSKVLIYDNEAREAGQRPVEEFELCLPDGDVSIHGAVGASELANTAKADVPYILKMESHPHTTCWPGRTLYLLAPSFPDKQRWVTALESVVAGGRVSREKAEADAKLLGNSLLKLEGDDRLDMNCTLPFSDQVVLVGTEEGLYALNVLKNSLTHIPGIGAVFQIYIIKDLEKLLMIAGEERALCLVDVKKVKQSLAQSHLPAQPDVSPNIFEAVKGCHLFAAGKIENSLCICAAMPSKVVILRYNDNLSKYCIRKEIETSEPCSCIHFTNYSILIGTNKFYEIDMKQYTLDEFLDKNDHSLAPAVFASSSNSFPVSIVQANSAGQREEYLLCFHEFGVFVDSYGRRSRTDDLKWSRLPLAFAYREPYLFVTHFNSLEVIEIQARSSLGSPARAYLEIPNPRYLGPAISSGAIYLASSYQDKLRVICCKGNLVKESGTEQHRVPSTSRSSPNKRGPPTYNEHITKRVASSPAPPEGPSHPREPSTPHRYRDREGRTELRRDKSPGRPLEREKSPGRMLSTRRERSPGRLFEDSSRGRLPAGAVRTPLSQVNKVWDQSSV

Target ORF information:

RefSeq Version	NM_007708.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus citron (Cit), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_007708.3

ORF Insert Sequence:

ATGTTGAAGT TCAAGTATGG TGTGCGGAAC CCGCCGGAGG CCAGTGCCTC CGAGCCCATT 
GCCAGTCGGG CCTCCAGGCT AAATCTCTTC TTCCAGGGGA AACCGCCCCT CATGACTCAA 
CAGCAGATGT CTGCTCTTTC CCGGGAAGGG ATGCTAGACG CCCTCTTCGC TCTCTTTGAA 
GAGTGCAGCC AACCCGCCCT GATGAAGATG AAGCACGTGA GCAGCTTTGT CCAGAAGTAT 
TCCGACACCA TAGCCGAGTT GCGGGAGCTG CAGCCGTCGG CGAGAGACTT CGAAGTTCGA 
AGCCTTGTGG GCTGTGGTCA CTTCGCTGAA GTGCAGGTGG TTAGAGAGAA GGCGACCGGG 
GACGTCTATG CCATGAAAAT CATGAAGAAG AAGGCTTTGC TGGCCCAGGA ACAGGTTTCA 
TTTTTCGAGG AGGAGAGGAA CATATTATCT CGGAGCACGA GTCCTTGGAT CCCCCAGTTA 
CAGTACGCCT TTCAGGACAA AAATAACCTT TACCTGGTCA TGGAATATCA GCCTGGAGGG 
GATTTGCTGT CGCTTCTGAA CAGATACGAG GACCAATTAG ATGAGAGCAT GATCCAGTTT 
TACCTTGCTG AGCTGATTTT GGCTGTCCAC AGCGTGCACC AGATGGGATA TGTGCATCGA 
GACATCAAGC CCGAGAACAT CCTCATCGAC CGGACGGGAC ACATCAAGCT GGTGGATTTT 
GGATCAGCCG CTAAGATGAA TTCAAATAAG GTGGATGCCA AACTCCCCAT TGGGACCCCG 
GATTACATGG CTCCGGAAGT GTTGACCGTG ATGAACGAGG ACCGAAGGGG CACATACGGC 
TTGGACTGTG ACTGGTGGTC TGTCGGAGTT GTTGCTTATG AGATGGTTTA TGGGAAGACC 
CCATTCACAG AGGGAACCTC CGCCCGGACC TTCAACAACA TCATGAACTT CCAGCGGTTT 
TTGAAGTTCC CAGATGACCC CAAAGTTAGC AGTGAGCTCC TTGATCTGCT TCAGAGTCTG 
CTGTGTGTCC AGAAAGAGAG ACTGAAGTTC GAGGGTCTCT GCTGCCACCC TTTCTTTGCC 
AGAACGGACT GGAACAACAT CCGTAACTCT CCTCCCCCCT TCGTCCCCAC CCTCAAGTCT 
GACGATGACA CCTCCAATTT TGATGAACCA GAGAAGAATT CGTGGGTTTC ATCCTCTGTG 
TGCCAGCTGA GCCCCTCGGG CTTCTCAGGC GAAGAGCTGC CGTTTGTGGG ATTTTCGTAC 
AGCAAGGCAC TGGGGTATCT TGGTAGATCT GAGTCTGTCG TGTCGAGTCT GGACTCCCCT 
GCCAAGGTTA GCTCCATGGA AAAGAAACTT CTCATCAAAA GCAAAGAGCT CCAAGACTCC 
CAGGACAAGT GTCACAAGAT GGAGCAGGAA ATGACCCGGT TACATCGCAG AGTGTCAGAG 
GTGGAGGCTG TGCTTAGTCA GAAGGAGGTG GAGCTGAAGG CCTCTGAGAC TCAGAGATCC 
CTCCTGGAGC AGGACCTTGC TACCTACATC ACAGAATGCA GTAGCTTAAA GCGAAGTTTG 
GAGCAAGCGC GGATGGAGGT GTCCCAGGAG GATGACAAAG CTCTGCAGCT TCTCCACGAC 
ATCCGAGAGC AGAGCCGGAA GCTCCAGGAG ATCAAGGAGC AGGAGTACCA GGCTCAGGTG 
GAGGAGATGA GGCTGATGAT GAATCAGCTG GAAGAAGACC TGGTGTCAGC CCGCAGACGC 
AGCGATCTCT ACGAGTCTGA GCTGAGGGAG TCTCGGCTTG CCGCCGAGGA ATTCAAGCGG 
AAGGCAAACG AATGTCAGCA CAAACTGATG AAGGCTAAGG ACCAAGGGAA GCCTGAAGTG 
GGAGAATATT CCAAACTGGA GAAGATCAAT GCTGAGCAGC AGCTGAAGAT CCAGGAGCTC 
CAGGAGAAGC TGGAGAAGGC TGTAAAAGCC AGCACAGAGG CCACCGAGCT CCTGCAGAAT 
ATCCGCCAGG CAAAGGAGCG AGCAGAGCGG GAGCTAGAGA AGCTACACAA CCGGGAAGAC 
TCCTCCGAGG GCATCAAAAA GAAGCTGGTG GAAGCCGAGG AACGCCGCCA CTCCCTGGAG 
AACAAGGTAA AGAGACTAGA GACCATGGAG CGTAGAGAGA ACAGACTGAA GGATGACATC 
CAGACAAAGT CCGAACAGAT CCAGCAGATG GCTGATAAAA TTCTGGAGCT GGAGGAGAAA 
CATCGGGAGG CTCAGGTCTC AGCTCAACAT CTAGAAGTAC ACTTGAAACA GAAAGAACAG 
CACTACGAGG AAAAGATCAA GGTATTGGAC AATCAGATAA AGAAGGACCT GGCCGACAAG 
GAGAGCCTGG AGAACATGAT GCAGAGACAC GAGGAGGAGG CCCACGAGAA GGGCAAGATC 
CTCAGCGAGC AGAAGGCGAT GATCAACGCG ATGGATTCCA AGATCCGATC CCTGGAGCAG 
AGGATCGTGG AGCTGTCGGA AGCCAACAAG CTTGCGGCAA ACAGCAGTCT CTTCACCCAG 
AGGAACATGA AGGCCCAGGA AGAGATGATC TCAGAACTCA GGCAGCAGAA ATTTTACCTG 
GAGACGCAGG CCGGAAAGCT GGAGGCCCAG AACCGAAAGC TGGAAGAGCA ACTGGAGAAG 
ATCAGCCACC AAGATCACAG TGACAAGAGT CGGCTGCTGG AGCTGGAAAC AAGGCTGAGG 
GAGGTCAGCC TGGAGCACGA GGAGCAGAAG CTGGAGCTGA AGCGGCAGCT CACGGAGCTG 
CAGCTGTCCC TGCAGGAGCG CGAGTCCCAG CTGACGGCCC TGCAGGCCGC CCGGGCAGCG 
CTGGAGAGCC AGCTCCGCCA GGCGAAGACA GAGCTGGAGG AGACAACCGC GGAAGCGGAG 
GAGGAGATCC AGGCGCTCAC GGCACATCGA GATGAGATCC AGCGCAAATT CGATGCCCTT 
CGCAACAGCT GCACGGTCAT CACCGACCTG GAGGAGCAGC TGAACCAGCT CACCGAGGAC 
AACGCCGAGC TCAACAACCA AAACTTCTAC CTGTCCAAAC AACTCGATGA GGCTTCCGGG 
GCCAATGACG AGATTGTGCA GCTGCGGAGT GAGGTGGACC ATCTCCGCCG TGAGATCACG 
GAGCGGGAGA TGCAGCTCAC CAGCCAGAAG CAAACGATGG AGGCTCTGAA GACGACATGC 
ACCATGCTGG AGGAGCAGGT CCTGGACCTG GAGGCCCTGA ACGACGAGCT GCTGGAGAAG 
GAGCGCCAGT GGGAGGCCTG GCGGAGCGTC CTCGGCGACG AGAAGTCCCA GTTCGAGTGT 
CGAGTTCGAG AGCTACAGAG GATGCTGGAC ACCGAGAAGC AGAGCAGGGC TAGGGCCGAT 
CAGCGGATCA CCGAGTCGCG CCAGGTGGTG GAGTTGGCGG TGAAGGAACA CAAGGCCGAG 
ATTCTTGCTC TGCAGCAGGC TCTCAAGGAG CAGAAGCTCA AAGCCGAGAG CCTGTCGGAC 
AAGCTCAACG ACCTGGAGAA GAAGCACGCC ATGCTGGAGA TGAACGCCCG GAGCTTACAG 
CAGAAACTAG AGACAGAGCG GGAGCTCAAA CAGAGGCTTC TGGAGGAGCA AGCCAAATTG 
CAGCAGCAGA TGGACCTGCA GAAGAACCAC ATCTTCAGAC TGACGCAAGG GCTGCAGGAG 
GCGCTGGACC GGGCGGATCT GCTGAAGACA GAAAGGAGCG ACCTGGAGTA CCAGCTGGAA 
AACATTCAGG TTCTCTACTC TCACGAGAAA GTGAAAATGG AAGGCACAAT CTCTCAGCAA 
ACCAAACTCA TTGATTTCCT GCAAGCCAAA ATGGACCAGC CTGCTAAAAA GAAAAAGGTT 
CCTCTGCAGT ACAATGAGCT GAAGCTAGCC CTGGAAAAGG AGAAAGCCCG ATGCGCGGAG 
CTGGAGGAGG CCCTTCAGAA GACCCGCATC GAACTCCGCT CTGCGCGGGA GGAAGCTGCC 
CACCGCAAAG CCACGGACCA CCCGCACCCG TCTACGCCAG CCACTGCGAG GCAGCAGATC 
GCCATGTCTG CCATTGTGCG GTCGCCCGAG CACCAGCCCA GTGCCATGAG CCTGCTTGCC 
CCACCATCCA GCCGCAGAAA GGAGTCGTCA ACTCCAGAGG AATTCAGCCG GCGTCTGAAA 
GAGCGCATGC ACCACAACAT CCCTCACCGG TTTAATGTGG GCCTGAACAT GAGAGCCACC 
AAGTGCGCCG TGTGTCTGGA TACTGTGCAC TTTGGACGCC AGGCATCCAA ATGCCTCGAA 
TGTCAAGTGA TGTGTCACCC CAAATGCTCC ACCTGCTTGC CCGCTACCTG TGGCCTGCCA 
GCTGAATATG CCACACACTT CACTGAGGCC TTCTGCCGGG ACAAAATGAA CTCCCCGGGG 
CTCCAGAGCA AGGAGCCTGG CAGCAGCTTG CACCTGGAAG GGTGGATGAA AGTGCCCAGA 
AATAACAAAC GGGGACAGCA AGGCTGGGAC AGGAAGTACA TTGTTCTGGA GGGGTCAAAA 
GTCCTCATCT ATGACAATGA AGCCAGAGAA GCTGGACAGA GGCCGGTGGA AGAATTTGAG 
CTGTGCCTTC CTGACGGGGA CGTATCTATT CATGGTGCCG TTGGTGCTTC AGAACTTGCA 
AATACCGCCA AAGCAGATGT CCCATACATC CTGAAGATGG AGTCTCACCC ACACACCACC 
TGCTGGCCTG GGAGAACCCT CTACTTGCTA GCACCCAGCT TCCCCGACAA GCAGCGCTGG 
GTCACCGCCT TAGAATCTGT CGTCGCAGGT GGGAGAGTTT CTAGGGAAAA GGCCGAAGCC 
GATGCTAAAT TACTTGGAAA CTCTCTGCTG AAACTGGAGG GCGATGACCG GCTTGACATG 
AACTGCACCC TGCCCTTCAG TGACCAGGTA GTGCTGGTGG GCACGGAGGA AGGCCTCTAC 
GCCCTGAATG TCTTGAAAAA CTCCTTAACC CACATCCCAG GGATTGGCGC AGTCTTCCAA 
ATTTACATCA TCAAGGACCT GGAGAAGCTG CTCATGATAG CAGGGGAAGA GCGGGCTCTG 
TGCCTGGTGG ACGTGAAGAA GGTGAAGCAG TCCCTGGCGC AGTCACACCT GCCTGCCCAG 
CCCGACGTCT CCCCCAACAT ATTCGAAGCC GTCAAAGGCT GCCACTTGTT CGCTGCTGGC 
AAGATCGAGA ACAGCCTGTG CATCTGCGCC GCTATGCCAA GCAAAGTCGT CATCCTCCGC 
TACAATGACA ACCTCAGCAA GTACTGCATC CGCAAGGAGA TCGAGACCTC AGAGCCCTGC 
AGCTGTATCC ACTTCACCAA CTACAGCATC CTCATTGGGA CCAACAAATT CTATGAGATC 
GACATGAAGC AGTACACGCT TGACGAGTTC CTGGACAAGA ACGACCATTC CTTGGCACCT 
GCTGTGTTCG CCTCCTCGTC CAACAGCTTC CCTGTCTCCA TTGTGCAGGC GAACAGCGCC 
GGGCAGCGAG AAGAATACCT GCTGTGCTTC CACGAATTTG GGGTGTTCGT GGATTCTTAC 
GGAAGACGTA GCCGCACAGA TGATCTCAAG TGGAGTCGCT TACCTCTGGC CTTCGCCTAC 
AGAGAACCTT ATCTGTTTGT GACTCACTTC AACTCCCTGG AAGTCATTGA GATCCAGGCC 
AGATCCTCAC TGGGGAGCCC TGCCCGAGCA TATCTGGAAA TTCCAAACCC TCGCTACCTG 
GGCCCCGCGA TTTCCTCCGG AGCGATTTAC CTGGCCTCCT CATACCAGGA CAAGTTAAGG 
GTCATATGCT GCAAAGGAAA CCTCGTGAAG GAGTCAGGCA CTGAGCAGCA CCGGGTGCCC 
TCCACCTCCC GCAGCAGCCC CAACAAGCGA GGCCCACCAA CATACAACGA GCACATCACC 
AAACGCGTGG CCTCCAGCCC GGCGCCACCG GAAGGCCCCA GCCACCCCCG AGAGCCAAGC 
ACACCGCACC GCTACCGAGA CAGAGAGGGT CGGACAGAGC TGCGCAGGGA CAAGTCTCCA 
GGCCGCCCTC TGGAGCGGGA GAAGTCCCCA GGCCGAATGC TCAGCACTAG GAGAGAGCGG 
TCCCCAGGGA GACTGTTTGA AGACAGCAGC AGGGGCCGGC TGCCTGCAGG AGCAGTGAGG 
ACCCCACTGT CCCAGGTTAA CAAGGTCTGG GACCAGTCTT CAGTATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cit ( XM_006530141.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006530204.1 Mus musculus citron (Cit), transcript variant X12, mRNA.

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CloneID	OMu00646
Clone ID Related Accession (Same CDS sequence)	XM_006530141.2 , XM_006530141.4 , NM_007708.3 , XM_006530141.3
Accession Version	XM_006530141.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6168bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-02-08
Organism	Mus musculus(House mouse)
Product	citron Rho-interacting kinase isoform X1
Comment

ATGTTGAAGT TCAAGTATGG TGTGCGGAAC CCGCCGGAGG CCAGTGCCTC CGAGCCCATT 
GCCAGTCGGG CCTCCAGGCT AAATCTCTTC TTCCAGGGGA AACCGCCCCT CATGACTCAA 
CAGCAGATGT CTGCTCTTTC CCGGGAAGGG ATGCTAGACG CCCTCTTCGC TCTCTTTGAA 
GAGTGCAGCC AACCCGCCCT GATGAAGATG AAGCACGTGA GCAGCTTTGT CCAGAAGTAT 
TCCGACACCA TAGCCGAGTT GCGGGAGCTG CAGCCGTCGG CGAGAGACTT CGAAGTTCGA 
AGCCTTGTGG GCTGTGGTCA CTTCGCTGAA GTGCAGGTGG TTAGAGAGAA GGCGACCGGG 
GACGTCTATG CCATGAAAAT CATGAAGAAG AAGGCTTTGC TGGCCCAGGA ACAGGTTTCA 
TTTTTCGAGG AGGAGAGGAA CATATTATCT CGGAGCACGA GTCCTTGGAT CCCCCAGTTA 
CAGTACGCCT TTCAGGACAA AAATAACCTT TACCTGGTCA TGGAATATCA GCCTGGAGGG 
GATTTGCTGT CGCTTCTGAA CAGATACGAG GACCAATTAG ATGAGAGCAT GATCCAGTTT 
TACCTTGCTG AGCTGATTTT GGCTGTCCAC AGCGTGCACC AGATGGGATA TGTGCATCGA 
GACATCAAGC CCGAGAACAT CCTCATCGAC CGGACGGGAC ACATCAAGCT GGTGGATTTT 
GGATCAGCCG CTAAGATGAA TTCAAATAAG GTGGATGCCA AACTCCCCAT TGGGACCCCG 
GATTACATGG CTCCGGAAGT GTTGACCGTG ATGAACGAGG ACCGAAGGGG CACATACGGC 
TTGGACTGTG ACTGGTGGTC TGTCGGAGTT GTTGCTTATG AGATGGTTTA TGGGAAGACC 
CCATTCACAG AGGGAACCTC CGCCCGGACC TTCAACAACA TCATGAACTT CCAGCGGTTT 
TTGAAGTTCC CAGATGACCC CAAAGTTAGC AGTGAGCTCC TTGATCTGCT TCAGAGTCTG 
CTGTGTGTCC AGAAAGAGAG ACTGAAGTTC GAGGGTCTCT GCTGCCACCC TTTCTTTGCC 
AGAACGGACT GGAACAACAT CCGTAACTCT CCTCCCCCCT TCGTCCCCAC CCTCAAGTCT 
GACGATGACA CCTCCAATTT TGATGAACCA GAGAAGAATT CGTGGGTTTC ATCCTCTGTG 
TGCCAGCTGA GCCCCTCGGG CTTCTCAGGC GAAGAGCTGC CGTTTGTGGG ATTTTCGTAC 
AGCAAGGCAC TGGGGTATCT TGGTAGATCT GAGTCTGTCG TGTCGAGTCT GGACTCCCCT 
GCCAAGGTTA GCTCCATGGA AAAGAAACTT CTCATCAAAA GCAAAGAGCT CCAAGACTCC 
CAGGACAAGT GTCACAAGAT GGAGCAGGAA ATGACCCGGT TACATCGCAG AGTGTCAGAG 
GTGGAGGCTG TGCTTAGTCA GAAGGAGGTG GAGCTGAAGG CCTCTGAGAC TCAGAGATCC 
CTCCTGGAGC AGGACCTTGC TACCTACATC ACAGAATGCA GTAGCTTAAA GCGAAGTTTG 
GAGCAAGCGC GGATGGAGGT GTCCCAGGAG GATGACAAAG CTCTGCAGCT TCTCCACGAC 
ATCCGAGAGC AGAGCCGGAA GCTCCAGGAG ATCAAGGAGC AGGAGTACCA GGCTCAGGTG 
GAGGAGATGA GGCTGATGAT GAATCAGCTG GAAGAAGACC TGGTGTCAGC CCGCAGACGC 
AGCGATCTCT ACGAGTCTGA GCTGAGGGAG TCTCGGCTTG CCGCCGAGGA ATTCAAGCGG 
AAGGCAAACG AATGTCAGCA CAAACTGATG AAGGCTAAGG ACCAAGGGAA GCCTGAAGTG 
GGAGAATATT CCAAACTGGA GAAGATCAAT GCTGAGCAGC AGCTGAAGAT CCAGGAGCTC 
CAGGAGAAGC TGGAGAAGGC TGTAAAAGCC AGCACAGAGG CCACCGAGCT CCTGCAGAAT 
ATCCGCCAGG CAAAGGAGCG AGCAGAGCGG GAGCTAGAGA AGCTACACAA CCGGGAAGAC 
TCCTCCGAGG GCATCAAAAA GAAGCTGGTG GAAGCCGAGG AACGCCGCCA CTCCCTGGAG 
AACAAGGTAA AGAGACTAGA GACCATGGAG CGTAGAGAGA ACAGACTGAA GGATGACATC 
CAGACAAAGT CCGAACAGAT CCAGCAGATG GCTGATAAAA TTCTGGAGCT GGAGGAGAAA 
CATCGGGAGG CTCAGGTCTC AGCTCAACAT CTAGAAGTAC ACTTGAAACA GAAAGAACAG 
CACTACGAGG AAAAGATCAA GGTATTGGAC AATCAGATAA AGAAGGACCT GGCCGACAAG 
GAGAGCCTGG AGAACATGAT GCAGAGACAC GAGGAGGAGG CCCACGAGAA GGGCAAGATC 
CTCAGCGAGC AGAAGGCGAT GATCAACGCG ATGGATTCCA AGATCCGATC CCTGGAGCAG 
AGGATCGTGG AGCTGTCGGA AGCCAACAAG CTTGCGGCAA ACAGCAGTCT CTTCACCCAG 
AGGAACATGA AGGCCCAGGA AGAGATGATC TCAGAACTCA GGCAGCAGAA ATTTTACCTG 
GAGACGCAGG CCGGAAAGCT GGAGGCCCAG AACCGAAAGC TGGAAGAGCA ACTGGAGAAG 
ATCAGCCACC AAGATCACAG TGACAAGAGT CGGCTGCTGG AGCTGGAAAC AAGGCTGAGG 
GAGGTCAGCC TGGAGCACGA GGAGCAGAAG CTGGAGCTGA AGCGGCAGCT CACGGAGCTG 
CAGCTGTCCC TGCAGGAGCG CGAGTCCCAG CTGACGGCCC TGCAGGCCGC CCGGGCAGCG 
CTGGAGAGCC AGCTCCGCCA GGCGAAGACA GAGCTGGAGG AGACAACCGC GGAAGCGGAG 
GAGGAGATCC AGGCGCTCAC GGCACATCGA GATGAGATCC AGCGCAAATT CGATGCCCTT 
CGCAACAGCT GCACGGTCAT CACCGACCTG GAGGAGCAGC TGAACCAGCT CACCGAGGAC 
AACGCCGAGC TCAACAACCA AAACTTCTAC CTGTCCAAAC AACTCGATGA GGCTTCCGGG 
GCCAATGACG AGATTGTGCA GCTGCGGAGT GAGGTGGACC ATCTCCGCCG TGAGATCACG 
GAGCGGGAGA TGCAGCTCAC CAGCCAGAAG CAAACGATGG AGGCTCTGAA GACGACATGC 
ACCATGCTGG AGGAGCAGGT CCTGGACCTG GAGGCCCTGA ACGACGAGCT GCTGGAGAAG 
GAGCGCCAGT GGGAGGCCTG GCGGAGCGTC CTCGGCGACG AGAAGTCCCA GTTCGAGTGT 
CGAGTTCGAG AGCTACAGAG GATGCTGGAC ACCGAGAAGC AGAGCAGGGC TAGGGCCGAT 
CAGCGGATCA CCGAGTCGCG CCAGGTGGTG GAGTTGGCGG TGAAGGAACA CAAGGCCGAG 
ATTCTTGCTC TGCAGCAGGC TCTCAAGGAG CAGAAGCTCA AAGCCGAGAG CCTGTCGGAC 
AAGCTCAACG ACCTGGAGAA GAAGCACGCC ATGCTGGAGA TGAACGCCCG GAGCTTACAG 
CAGAAACTAG AGACAGAGCG GGAGCTCAAA CAGAGGCTTC TGGAGGAGCA AGCCAAATTG 
CAGCAGCAGA TGGACCTGCA GAAGAACCAC ATCTTCAGAC TGACGCAAGG GCTGCAGGAG 
GCGCTGGACC GGGCGGATCT GCTGAAGACA GAAAGGAGCG ACCTGGAGTA CCAGCTGGAA 
AACATTCAGG TTCTCTACTC TCACGAGAAA GTGAAAATGG AAGGCACAAT CTCTCAGCAA 
ACCAAACTCA TTGATTTCCT GCAAGCCAAA ATGGACCAGC CTGCTAAAAA GAAAAAGGTT 
CCTCTGCAGT ACAATGAGCT GAAGCTAGCC CTGGAAAAGG AGAAAGCCCG ATGCGCGGAG 
CTGGAGGAGG CCCTTCAGAA GACCCGCATC GAACTCCGCT CTGCGCGGGA GGAAGCTGCC 
CACCGCAAAG CCACGGACCA CCCGCACCCG TCTACGCCAG CCACTGCGAG GCAGCAGATC 
GCCATGTCTG CCATTGTGCG GTCGCCCGAG CACCAGCCCA GTGCCATGAG CCTGCTTGCC 
CCACCATCCA GCCGCAGAAA GGAGTCGTCA ACTCCAGAGG AATTCAGCCG GCGTCTGAAA 
GAGCGCATGC ACCACAACAT CCCTCACCGG TTTAATGTGG GCCTGAACAT GAGAGCCACC 
AAGTGCGCCG TGTGTCTGGA TACTGTGCAC TTTGGACGCC AGGCATCCAA ATGCCTCGAA 
TGTCAAGTGA TGTGTCACCC CAAATGCTCC ACCTGCTTGC CCGCTACCTG TGGCCTGCCA 
GCTGAATATG CCACACACTT CACTGAGGCC TTCTGCCGGG ACAAAATGAA CTCCCCGGGG 
CTCCAGAGCA AGGAGCCTGG CAGCAGCTTG CACCTGGAAG GGTGGATGAA AGTGCCCAGA 
AATAACAAAC GGGGACAGCA AGGCTGGGAC AGGAAGTACA TTGTTCTGGA GGGGTCAAAA 
GTCCTCATCT ATGACAATGA AGCCAGAGAA GCTGGACAGA GGCCGGTGGA AGAATTTGAG 
CTGTGCCTTC CTGACGGGGA CGTATCTATT CATGGTGCCG TTGGTGCTTC AGAACTTGCA 
AATACCGCCA AAGCAGATGT CCCATACATC CTGAAGATGG AGTCTCACCC ACACACCACC 
TGCTGGCCTG GGAGAACCCT CTACTTGCTA GCACCCAGCT TCCCCGACAA GCAGCGCTGG 
GTCACCGCCT TAGAATCTGT CGTCGCAGGT GGGAGAGTTT CTAGGGAAAA GGCCGAAGCC 
GATGCTAAAT TACTTGGAAA CTCTCTGCTG AAACTGGAGG GCGATGACCG GCTTGACATG 
AACTGCACCC TGCCCTTCAG TGACCAGGTA GTGCTGGTGG GCACGGAGGA AGGCCTCTAC 
GCCCTGAATG TCTTGAAAAA CTCCTTAACC CACATCCCAG GGATTGGCGC AGTCTTCCAA 
ATTTACATCA TCAAGGACCT GGAGAAGCTG CTCATGATAG CAGGGGAAGA GCGGGCTCTG 
TGCCTGGTGG ACGTGAAGAA GGTGAAGCAG TCCCTGGCGC AGTCACACCT GCCTGCCCAG 
CCCGACGTCT CCCCCAACAT ATTCGAAGCC GTCAAAGGCT GCCACTTGTT CGCTGCTGGC 
AAGATCGAGA ACAGCCTGTG CATCTGCGCC GCTATGCCAA GCAAAGTCGT CATCCTCCGC 
TACAATGACA ACCTCAGCAA GTACTGCATC CGCAAGGAGA TCGAGACCTC AGAGCCCTGC 
AGCTGTATCC ACTTCACCAA CTACAGCATC CTCATTGGGA CCAACAAATT CTATGAGATC 
GACATGAAGC AGTACACGCT TGACGAGTTC CTGGACAAGA ACGACCATTC CTTGGCACCT 
GCTGTGTTCG CCTCCTCGTC CAACAGCTTC CCTGTCTCCA TTGTGCAGGC GAACAGCGCC 
GGGCAGCGAG AAGAATACCT GCTGTGCTTC CACGAATTTG GGGTGTTCGT GGATTCTTAC 
GGAAGACGTA GCCGCACAGA TGATCTCAAG TGGAGTCGCT TACCTCTGGC CTTCGCCTAC 
AGAGAACCTT ATCTGTTTGT GACTCACTTC AACTCCCTGG AAGTCATTGA GATCCAGGCC 
AGATCCTCAC TGGGGAGCCC TGCCCGAGCA TATCTGGAAA TTCCAAACCC TCGCTACCTG 
GGCCCCGCGA TTTCCTCCGG AGCGATTTAC CTGGCCTCCT CATACCAGGA CAAGTTAAGG 
GTCATATGCT GCAAAGGAAA CCTCGTGAAG GAGTCAGGCA CTGAGCAGCA CCGGGTGCCC 
TCCACCTCCC GCAGCAGCCC CAACAAGCGA GGCCCACCAA CATACAACGA GCACATCACC 
AAACGCGTGG CCTCCAGCCC GGCGCCACCG GAAGGCCCCA GCCACCCCCG AGAGCCAAGC 
ACACCGCACC GCTACCGAGA CAGAGAGGGT CGGACAGAGC TGCGCAGGGA CAAGTCTCCA 
GGCCGCCCTC TGGAGCGGGA GAAGTCCCCA GGCCGAATGC TCAGCACTAG GAGAGAGCGG 
TCCCCAGGGA GACTGTTTGA AGACAGCAGC AGGGGCCGGC TGCCTGCAGG AGCAGTGAGG 
ACCCCACTGT CCCAGGTTAA CAAGGTCTGG GACCAGTCTT CAGTATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq

XP_006530204.1

Translation

MLKFKYGVRNPPEASASEPIASRASRLNLFFQGKPPLMTQQQMSALSREGMLDALFALFEECSQPALMKMKHVSSFVQKYSDTIAELRELQPSARDFEVRSLVGCGHFAEVQVVREKATGDVYAMKIMKKKALLAQEQVSFFEEERNILSRSTSPWIPQLQYAFQDKNNLYLVMEYQPGGDLLSLLNRYEDQLDESMIQFYLAELILAVHSVHQMGYVHRDIKPENILIDRTGHIKLVDFGSAAKMNSNKVDAKLPIGTPDYMAPEVLTVMNEDRRGTYGLDCDWWSVGVVAYEMVYGKTPFTEGTSARTFNNIMNFQRFLKFPDDPKVSSELLDLLQSLLCVQKERLKFEGLCCHPFFARTDWNNIRNSPPPFVPTLKSDDDTSNFDEPEKNSWVSSSVCQLSPSGFSGEELPFVGFSYSKALGYLGRSESVVSSLDSPAKVSSMEKKLLIKSKELQDSQDKCHKMEQEMTRLHRRVSEVEAVLSQKEVELKASETQRSLLEQDLATYITECSSLKRSLEQARMEVSQEDDKALQLLHDIREQSRKLQEIKEQEYQAQVEEMRLMMNQLEEDLVSARRRSDLYESELRESRLAAEEFKRKANECQHKLMKAKDQGKPEVGEYSKLEKINAEQQLKIQELQEKLEKAVKASTEATELLQNIRQAKERAERELEKLHNREDSSEGIKKKLVEAEERRHSLENKVKRLETMERRENRLKDDIQTKSEQIQQMADKILELEEKHREAQVSAQHLEVHLKQKEQHYEEKIKVLDNQIKKDLADKESLENMMQRHEEEAHEKGKILSEQKAMINAMDSKIRSLEQRIVELSEANKLAANSSLFTQRNMKAQEEMISELRQQKFYLETQAGKLEAQNRKLEEQLEKISHQDHSDKSRLLELETRLREVSLEHEEQKLELKRQLTELQLSLQERESQLTALQAARAALESQLRQAKTELEETTAEAEEEIQALTAHRDEIQRKFDALRNSCTVITDLEEQLNQLTEDNAELNNQNFYLSKQLDEASGANDEIVQLRSEVDHLRREITEREMQLTSQKQTMEALKTTCTMLEEQVLDLEALNDELLEKERQWEAWRSVLGDEKSQFECRVRELQRMLDTEKQSRARADQRITESRQVVELAVKEHKAEILALQQALKEQKLKAESLSDKLNDLEKKHAMLEMNARSLQQKLETERELKQRLLEEQAKLQQQMDLQKNHIFRLTQGLQEALDRADLLKTERSDLEYQLENIQVLYSHEKVKMEGTISQQTKLIDFLQAKMDQPAKKKKVPLQYNELKLALEKEKARCAELEEALQKTRIELRSAREEAAHRKATDHPHPSTPATARQQIAMSAIVRSPEHQPSAMSLLAPPSSRRKESSTPEEFSRRLKERMHHNIPHRFNVGLNMRATKCAVCLDTVHFGRQASKCLECQVMCHPKCSTCLPATCGLPAEYATHFTEAFCRDKMNSPGLQSKEPGSSLHLEGWMKVPRNNKRGQQGWDRKYIVLEGSKVLIYDNEAREAGQRPVEEFELCLPDGDVSIHGAVGASELANTAKADVPYILKMESHPHTTCWPGRTLYLLAPSFPDKQRWVTALESVVAGGRVSREKAEADAKLLGNSLLKLEGDDRLDMNCTLPFSDQVVLVGTEEGLYALNVLKNSLTHIPGIGAVFQIYIIKDLEKLLMIAGEERALCLVDVKKVKQSLAQSHLPAQPDVSPNIFEAVKGCHLFAAGKIENSLCICAAMPSKVVILRYNDNLSKYCIRKEIETSEPCSCIHFTNYSILIGTNKFYEIDMKQYTLDEFLDKNDHSLAPAVFASSSNSFPVSIVQANSAGQREEYLLCFHEFGVFVDSYGRRSRTDDLKWSRLPLAFAYREPYLFVTHFNSLEVIEIQARSSLGSPARAYLEIPNPRYLGPAISSGAIYLASSYQDKLRVICCKGNLVKESGTEQHRVPSTSRSSPNKRGPPTYNEHITKRVASSPAPPEGPSHPREPSTPHRYRDREGRTELRRDKSPGRPLEREKSPGRMLSTRRERSPGRLFEDSSRGRLPAGAVRTPLSQVNKVWDQSSV

Target ORF information:

RefSeq Version	XM_006530141.2
Organism	Mus musculus(House mouse)
Definition	Mus musculus citron (Cit), transcript variant X12, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006530141.2

ORF Insert Sequence:

ATGTTGAAGT TCAAGTATGG TGTGCGGAAC CCGCCGGAGG CCAGTGCCTC CGAGCCCATT 
GCCAGTCGGG CCTCCAGGCT AAATCTCTTC TTCCAGGGGA AACCGCCCCT CATGACTCAA 
CAGCAGATGT CTGCTCTTTC CCGGGAAGGG ATGCTAGACG CCCTCTTCGC TCTCTTTGAA 
GAGTGCAGCC AACCCGCCCT GATGAAGATG AAGCACGTGA GCAGCTTTGT CCAGAAGTAT 
TCCGACACCA TAGCCGAGTT GCGGGAGCTG CAGCCGTCGG CGAGAGACTT CGAAGTTCGA 
AGCCTTGTGG GCTGTGGTCA CTTCGCTGAA GTGCAGGTGG TTAGAGAGAA GGCGACCGGG 
GACGTCTATG CCATGAAAAT CATGAAGAAG AAGGCTTTGC TGGCCCAGGA ACAGGTTTCA 
TTTTTCGAGG AGGAGAGGAA CATATTATCT CGGAGCACGA GTCCTTGGAT CCCCCAGTTA 
CAGTACGCCT TTCAGGACAA AAATAACCTT TACCTGGTCA TGGAATATCA GCCTGGAGGG 
GATTTGCTGT CGCTTCTGAA CAGATACGAG GACCAATTAG ATGAGAGCAT GATCCAGTTT 
TACCTTGCTG AGCTGATTTT GGCTGTCCAC AGCGTGCACC AGATGGGATA TGTGCATCGA 
GACATCAAGC CCGAGAACAT CCTCATCGAC CGGACGGGAC ACATCAAGCT GGTGGATTTT 
GGATCAGCCG CTAAGATGAA TTCAAATAAG GTGGATGCCA AACTCCCCAT TGGGACCCCG 
GATTACATGG CTCCGGAAGT GTTGACCGTG ATGAACGAGG ACCGAAGGGG CACATACGGC 
TTGGACTGTG ACTGGTGGTC TGTCGGAGTT GTTGCTTATG AGATGGTTTA TGGGAAGACC 
CCATTCACAG AGGGAACCTC CGCCCGGACC TTCAACAACA TCATGAACTT CCAGCGGTTT 
TTGAAGTTCC CAGATGACCC CAAAGTTAGC AGTGAGCTCC TTGATCTGCT TCAGAGTCTG 
CTGTGTGTCC AGAAAGAGAG ACTGAAGTTC GAGGGTCTCT GCTGCCACCC TTTCTTTGCC 
AGAACGGACT GGAACAACAT CCGTAACTCT CCTCCCCCCT TCGTCCCCAC CCTCAAGTCT 
GACGATGACA CCTCCAATTT TGATGAACCA GAGAAGAATT CGTGGGTTTC ATCCTCTGTG 
TGCCAGCTGA GCCCCTCGGG CTTCTCAGGC GAAGAGCTGC CGTTTGTGGG ATTTTCGTAC 
AGCAAGGCAC TGGGGTATCT TGGTAGATCT GAGTCTGTCG TGTCGAGTCT GGACTCCCCT 
GCCAAGGTTA GCTCCATGGA AAAGAAACTT CTCATCAAAA GCAAAGAGCT CCAAGACTCC 
CAGGACAAGT GTCACAAGAT GGAGCAGGAA ATGACCCGGT TACATCGCAG AGTGTCAGAG 
GTGGAGGCTG TGCTTAGTCA GAAGGAGGTG GAGCTGAAGG CCTCTGAGAC TCAGAGATCC 
CTCCTGGAGC AGGACCTTGC TACCTACATC ACAGAATGCA GTAGCTTAAA GCGAAGTTTG 
GAGCAAGCGC GGATGGAGGT GTCCCAGGAG GATGACAAAG CTCTGCAGCT TCTCCACGAC 
ATCCGAGAGC AGAGCCGGAA GCTCCAGGAG ATCAAGGAGC AGGAGTACCA GGCTCAGGTG 
GAGGAGATGA GGCTGATGAT GAATCAGCTG GAAGAAGACC TGGTGTCAGC CCGCAGACGC 
AGCGATCTCT ACGAGTCTGA GCTGAGGGAG TCTCGGCTTG CCGCCGAGGA ATTCAAGCGG 
AAGGCAAACG AATGTCAGCA CAAACTGATG AAGGCTAAGG ACCAAGGGAA GCCTGAAGTG 
GGAGAATATT CCAAACTGGA GAAGATCAAT GCTGAGCAGC AGCTGAAGAT CCAGGAGCTC 
CAGGAGAAGC TGGAGAAGGC TGTAAAAGCC AGCACAGAGG CCACCGAGCT CCTGCAGAAT 
ATCCGCCAGG CAAAGGAGCG AGCAGAGCGG GAGCTAGAGA AGCTACACAA CCGGGAAGAC 
TCCTCCGAGG GCATCAAAAA GAAGCTGGTG GAAGCCGAGG AACGCCGCCA CTCCCTGGAG 
AACAAGGTAA AGAGACTAGA GACCATGGAG CGTAGAGAGA ACAGACTGAA GGATGACATC 
CAGACAAAGT CCGAACAGAT CCAGCAGATG GCTGATAAAA TTCTGGAGCT GGAGGAGAAA 
CATCGGGAGG CTCAGGTCTC AGCTCAACAT CTAGAAGTAC ACTTGAAACA GAAAGAACAG 
CACTACGAGG AAAAGATCAA GGTATTGGAC AATCAGATAA AGAAGGACCT GGCCGACAAG 
GAGAGCCTGG AGAACATGAT GCAGAGACAC GAGGAGGAGG CCCACGAGAA GGGCAAGATC 
CTCAGCGAGC AGAAGGCGAT GATCAACGCG ATGGATTCCA AGATCCGATC CCTGGAGCAG 
AGGATCGTGG AGCTGTCGGA AGCCAACAAG CTTGCGGCAA ACAGCAGTCT CTTCACCCAG 
AGGAACATGA AGGCCCAGGA AGAGATGATC TCAGAACTCA GGCAGCAGAA ATTTTACCTG 
GAGACGCAGG CCGGAAAGCT GGAGGCCCAG AACCGAAAGC TGGAAGAGCA ACTGGAGAAG 
ATCAGCCACC AAGATCACAG TGACAAGAGT CGGCTGCTGG AGCTGGAAAC AAGGCTGAGG 
GAGGTCAGCC TGGAGCACGA GGAGCAGAAG CTGGAGCTGA AGCGGCAGCT CACGGAGCTG 
CAGCTGTCCC TGCAGGAGCG CGAGTCCCAG CTGACGGCCC TGCAGGCCGC CCGGGCAGCG 
CTGGAGAGCC AGCTCCGCCA GGCGAAGACA GAGCTGGAGG AGACAACCGC GGAAGCGGAG 
GAGGAGATCC AGGCGCTCAC GGCACATCGA GATGAGATCC AGCGCAAATT CGATGCCCTT 
CGCAACAGCT GCACGGTCAT CACCGACCTG GAGGAGCAGC TGAACCAGCT CACCGAGGAC 
AACGCCGAGC TCAACAACCA AAACTTCTAC CTGTCCAAAC AACTCGATGA GGCTTCCGGG 
GCCAATGACG AGATTGTGCA GCTGCGGAGT GAGGTGGACC ATCTCCGCCG TGAGATCACG 
GAGCGGGAGA TGCAGCTCAC CAGCCAGAAG CAAACGATGG AGGCTCTGAA GACGACATGC 
ACCATGCTGG AGGAGCAGGT CCTGGACCTG GAGGCCCTGA ACGACGAGCT GCTGGAGAAG 
GAGCGCCAGT GGGAGGCCTG GCGGAGCGTC CTCGGCGACG AGAAGTCCCA GTTCGAGTGT 
CGAGTTCGAG AGCTACAGAG GATGCTGGAC ACCGAGAAGC AGAGCAGGGC TAGGGCCGAT 
CAGCGGATCA CCGAGTCGCG CCAGGTGGTG GAGTTGGCGG TGAAGGAACA CAAGGCCGAG 
ATTCTTGCTC TGCAGCAGGC TCTCAAGGAG CAGAAGCTCA AAGCCGAGAG CCTGTCGGAC 
AAGCTCAACG ACCTGGAGAA GAAGCACGCC ATGCTGGAGA TGAACGCCCG GAGCTTACAG 
CAGAAACTAG AGACAGAGCG GGAGCTCAAA CAGAGGCTTC TGGAGGAGCA AGCCAAATTG 
CAGCAGCAGA TGGACCTGCA GAAGAACCAC ATCTTCAGAC TGACGCAAGG GCTGCAGGAG 
GCGCTGGACC GGGCGGATCT GCTGAAGACA GAAAGGAGCG ACCTGGAGTA CCAGCTGGAA 
AACATTCAGG TTCTCTACTC TCACGAGAAA GTGAAAATGG AAGGCACAAT CTCTCAGCAA 
ACCAAACTCA TTGATTTCCT GCAAGCCAAA ATGGACCAGC CTGCTAAAAA GAAAAAGGTT 
CCTCTGCAGT ACAATGAGCT GAAGCTAGCC CTGGAAAAGG AGAAAGCCCG ATGCGCGGAG 
CTGGAGGAGG CCCTTCAGAA GACCCGCATC GAACTCCGCT CTGCGCGGGA GGAAGCTGCC 
CACCGCAAAG CCACGGACCA CCCGCACCCG TCTACGCCAG CCACTGCGAG GCAGCAGATC 
GCCATGTCTG CCATTGTGCG GTCGCCCGAG CACCAGCCCA GTGCCATGAG CCTGCTTGCC 
CCACCATCCA GCCGCAGAAA GGAGTCGTCA ACTCCAGAGG AATTCAGCCG GCGTCTGAAA 
GAGCGCATGC ACCACAACAT CCCTCACCGG TTTAATGTGG GCCTGAACAT GAGAGCCACC 
AAGTGCGCCG TGTGTCTGGA TACTGTGCAC TTTGGACGCC AGGCATCCAA ATGCCTCGAA 
TGTCAAGTGA TGTGTCACCC CAAATGCTCC ACCTGCTTGC CCGCTACCTG TGGCCTGCCA 
GCTGAATATG CCACACACTT CACTGAGGCC TTCTGCCGGG ACAAAATGAA CTCCCCGGGG 
CTCCAGAGCA AGGAGCCTGG CAGCAGCTTG CACCTGGAAG GGTGGATGAA AGTGCCCAGA 
AATAACAAAC GGGGACAGCA AGGCTGGGAC AGGAAGTACA TTGTTCTGGA GGGGTCAAAA 
GTCCTCATCT ATGACAATGA AGCCAGAGAA GCTGGACAGA GGCCGGTGGA AGAATTTGAG 
CTGTGCCTTC CTGACGGGGA CGTATCTATT CATGGTGCCG TTGGTGCTTC AGAACTTGCA 
AATACCGCCA AAGCAGATGT CCCATACATC CTGAAGATGG AGTCTCACCC ACACACCACC 
TGCTGGCCTG GGAGAACCCT CTACTTGCTA GCACCCAGCT TCCCCGACAA GCAGCGCTGG 
GTCACCGCCT TAGAATCTGT CGTCGCAGGT GGGAGAGTTT CTAGGGAAAA GGCCGAAGCC 
GATGCTAAAT TACTTGGAAA CTCTCTGCTG AAACTGGAGG GCGATGACCG GCTTGACATG 
AACTGCACCC TGCCCTTCAG TGACCAGGTA GTGCTGGTGG GCACGGAGGA AGGCCTCTAC 
GCCCTGAATG TCTTGAAAAA CTCCTTAACC CACATCCCAG GGATTGGCGC AGTCTTCCAA 
ATTTACATCA TCAAGGACCT GGAGAAGCTG CTCATGATAG CAGGGGAAGA GCGGGCTCTG 
TGCCTGGTGG ACGTGAAGAA GGTGAAGCAG TCCCTGGCGC AGTCACACCT GCCTGCCCAG 
CCCGACGTCT CCCCCAACAT ATTCGAAGCC GTCAAAGGCT GCCACTTGTT CGCTGCTGGC 
AAGATCGAGA ACAGCCTGTG CATCTGCGCC GCTATGCCAA GCAAAGTCGT CATCCTCCGC 
TACAATGACA ACCTCAGCAA GTACTGCATC CGCAAGGAGA TCGAGACCTC AGAGCCCTGC 
AGCTGTATCC ACTTCACCAA CTACAGCATC CTCATTGGGA CCAACAAATT CTATGAGATC 
GACATGAAGC AGTACACGCT TGACGAGTTC CTGGACAAGA ACGACCATTC CTTGGCACCT 
GCTGTGTTCG CCTCCTCGTC CAACAGCTTC CCTGTCTCCA TTGTGCAGGC GAACAGCGCC 
GGGCAGCGAG AAGAATACCT GCTGTGCTTC CACGAATTTG GGGTGTTCGT GGATTCTTAC 
GGAAGACGTA GCCGCACAGA TGATCTCAAG TGGAGTCGCT TACCTCTGGC CTTCGCCTAC 
AGAGAACCTT ATCTGTTTGT GACTCACTTC AACTCCCTGG AAGTCATTGA GATCCAGGCC 
AGATCCTCAC TGGGGAGCCC TGCCCGAGCA TATCTGGAAA TTCCAAACCC TCGCTACCTG 
GGCCCCGCGA TTTCCTCCGG AGCGATTTAC CTGGCCTCCT CATACCAGGA CAAGTTAAGG 
GTCATATGCT GCAAAGGAAA CCTCGTGAAG GAGTCAGGCA CTGAGCAGCA CCGGGTGCCC 
TCCACCTCCC GCAGCAGCCC CAACAAGCGA GGCCCACCAA CATACAACGA GCACATCACC 
AAACGCGTGG CCTCCAGCCC GGCGCCACCG GAAGGCCCCA GCCACCCCCG AGAGCCAAGC 
ACACCGCACC GCTACCGAGA CAGAGAGGGT CGGACAGAGC TGCGCAGGGA CAAGTCTCCA 
GGCCGCCCTC TGGAGCGGGA GAAGTCCCCA GGCCGAATGC TCAGCACTAG GAGAGAGCGG 
TCCCCAGGGA GACTGTTTGA AGACAGCAGC AGGGGCCGGC TGCCTGCAGG AGCAGTGAGG 
ACCCCACTGT CCCAGGTTAA CAAGGTCTGG GACCAGTCTT CAGTATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cit ( XM_006530141.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006530204.1 Mus musculus citron (Cit), transcript variant X12, mRNA.

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CloneID	OMu00646
Clone ID Related Accession (Same CDS sequence)	XM_006530141.2 , XM_006530141.4 , NM_007708.3 , XM_006530141.3
Accession Version	XM_006530141.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6168bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	citron Rho-interacting kinase isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000071.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006530141.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTGAAGT TCAAGTATGG TGTGCGGAAC CCGCCGGAGG CCAGTGCCTC CGAGCCCATT 
GCCAGTCGGG CCTCCAGGCT AAATCTCTTC TTCCAGGGGA AACCGCCCCT CATGACTCAA 
CAGCAGATGT CTGCTCTTTC CCGGGAAGGG ATGCTAGACG CCCTCTTCGC TCTCTTTGAA 
GAGTGCAGCC AACCCGCCCT GATGAAGATG AAGCACGTGA GCAGCTTTGT CCAGAAGTAT 
TCCGACACCA TAGCCGAGTT GCGGGAGCTG CAGCCGTCGG CGAGAGACTT CGAAGTTCGA 
AGCCTTGTGG GCTGTGGTCA CTTCGCTGAA GTGCAGGTGG TTAGAGAGAA GGCGACCGGG 
GACGTCTATG CCATGAAAAT CATGAAGAAG AAGGCTTTGC TGGCCCAGGA ACAGGTTTCA 
TTTTTCGAGG AGGAGAGGAA CATATTATCT CGGAGCACGA GTCCTTGGAT CCCCCAGTTA 
CAGTACGCCT TTCAGGACAA AAATAACCTT TACCTGGTCA TGGAATATCA GCCTGGAGGG 
GATTTGCTGT CGCTTCTGAA CAGATACGAG GACCAATTAG ATGAGAGCAT GATCCAGTTT 
TACCTTGCTG AGCTGATTTT GGCTGTCCAC AGCGTGCACC AGATGGGATA TGTGCATCGA 
GACATCAAGC CCGAGAACAT CCTCATCGAC CGGACGGGAC ACATCAAGCT GGTGGATTTT 
GGATCAGCCG CTAAGATGAA TTCAAATAAG GTGGATGCCA AACTCCCCAT TGGGACCCCG 
GATTACATGG CTCCGGAAGT GTTGACCGTG ATGAACGAGG ACCGAAGGGG CACATACGGC 
TTGGACTGTG ACTGGTGGTC TGTCGGAGTT GTTGCTTATG AGATGGTTTA TGGGAAGACC 
CCATTCACAG AGGGAACCTC CGCCCGGACC TTCAACAACA TCATGAACTT CCAGCGGTTT 
TTGAAGTTCC CAGATGACCC CAAAGTTAGC AGTGAGCTCC TTGATCTGCT TCAGAGTCTG 
CTGTGTGTCC AGAAAGAGAG ACTGAAGTTC GAGGGTCTCT GCTGCCACCC TTTCTTTGCC 
AGAACGGACT GGAACAACAT CCGTAACTCT CCTCCCCCCT TCGTCCCCAC CCTCAAGTCT 
GACGATGACA CCTCCAATTT TGATGAACCA GAGAAGAATT CGTGGGTTTC ATCCTCTGTG 
TGCCAGCTGA GCCCCTCGGG CTTCTCAGGC GAAGAGCTGC CGTTTGTGGG ATTTTCGTAC 
AGCAAGGCAC TGGGGTATCT TGGTAGATCT GAGTCTGTCG TGTCGAGTCT GGACTCCCCT 
GCCAAGGTTA GCTCCATGGA AAAGAAACTT CTCATCAAAA GCAAAGAGCT CCAAGACTCC 
CAGGACAAGT GTCACAAGAT GGAGCAGGAA ATGACCCGGT TACATCGCAG AGTGTCAGAG 
GTGGAGGCTG TGCTTAGTCA GAAGGAGGTG GAGCTGAAGG CCTCTGAGAC TCAGAGATCC 
CTCCTGGAGC AGGACCTTGC TACCTACATC ACAGAATGCA GTAGCTTAAA GCGAAGTTTG 
GAGCAAGCGC GGATGGAGGT GTCCCAGGAG GATGACAAAG CTCTGCAGCT TCTCCACGAC 
ATCCGAGAGC AGAGCCGGAA GCTCCAGGAG ATCAAGGAGC AGGAGTACCA GGCTCAGGTG 
GAGGAGATGA GGCTGATGAT GAATCAGCTG GAAGAAGACC TGGTGTCAGC CCGCAGACGC 
AGCGATCTCT ACGAGTCTGA GCTGAGGGAG TCTCGGCTTG CCGCCGAGGA ATTCAAGCGG 
AAGGCAAACG AATGTCAGCA CAAACTGATG AAGGCTAAGG ACCAAGGGAA GCCTGAAGTG 
GGAGAATATT CCAAACTGGA GAAGATCAAT GCTGAGCAGC AGCTGAAGAT CCAGGAGCTC 
CAGGAGAAGC TGGAGAAGGC TGTAAAAGCC AGCACAGAGG CCACCGAGCT CCTGCAGAAT 
ATCCGCCAGG CAAAGGAGCG AGCAGAGCGG GAGCTAGAGA AGCTACACAA CCGGGAAGAC 
TCCTCCGAGG GCATCAAAAA GAAGCTGGTG GAAGCCGAGG AACGCCGCCA CTCCCTGGAG 
AACAAGGTAA AGAGACTAGA GACCATGGAG CGTAGAGAGA ACAGACTGAA GGATGACATC 
CAGACAAAGT CCGAACAGAT CCAGCAGATG GCTGATAAAA TTCTGGAGCT GGAGGAGAAA 
CATCGGGAGG CTCAGGTCTC AGCTCAACAT CTAGAAGTAC ACTTGAAACA GAAAGAACAG 
CACTACGAGG AAAAGATCAA GGTATTGGAC AATCAGATAA AGAAGGACCT GGCCGACAAG 
GAGAGCCTGG AGAACATGAT GCAGAGACAC GAGGAGGAGG CCCACGAGAA GGGCAAGATC 
CTCAGCGAGC AGAAGGCGAT GATCAACGCG ATGGATTCCA AGATCCGATC CCTGGAGCAG 
AGGATCGTGG AGCTGTCGGA AGCCAACAAG CTTGCGGCAA ACAGCAGTCT CTTCACCCAG 
AGGAACATGA AGGCCCAGGA AGAGATGATC TCAGAACTCA GGCAGCAGAA ATTTTACCTG 
GAGACGCAGG CCGGAAAGCT GGAGGCCCAG AACCGAAAGC TGGAAGAGCA ACTGGAGAAG 
ATCAGCCACC AAGATCACAG TGACAAGAGT CGGCTGCTGG AGCTGGAAAC AAGGCTGAGG 
GAGGTCAGCC TGGAGCACGA GGAGCAGAAG CTGGAGCTGA AGCGGCAGCT CACGGAGCTG 
CAGCTGTCCC TGCAGGAGCG CGAGTCCCAG CTGACGGCCC TGCAGGCCGC CCGGGCAGCG 
CTGGAGAGCC AGCTCCGCCA GGCGAAGACA GAGCTGGAGG AGACAACCGC GGAAGCGGAG 
GAGGAGATCC AGGCGCTCAC GGCACATCGA GATGAGATCC AGCGCAAATT CGATGCCCTT 
CGCAACAGCT GCACGGTCAT CACCGACCTG GAGGAGCAGC TGAACCAGCT CACCGAGGAC 
AACGCCGAGC TCAACAACCA AAACTTCTAC CTGTCCAAAC AACTCGATGA GGCTTCCGGG 
GCCAATGACG AGATTGTGCA GCTGCGGAGT GAGGTGGACC ATCTCCGCCG TGAGATCACG 
GAGCGGGAGA TGCAGCTCAC CAGCCAGAAG CAAACGATGG AGGCTCTGAA GACGACATGC 
ACCATGCTGG AGGAGCAGGT CCTGGACCTG GAGGCCCTGA ACGACGAGCT GCTGGAGAAG 
GAGCGCCAGT GGGAGGCCTG GCGGAGCGTC CTCGGCGACG AGAAGTCCCA GTTCGAGTGT 
CGAGTTCGAG AGCTACAGAG GATGCTGGAC ACCGAGAAGC AGAGCAGGGC TAGGGCCGAT 
CAGCGGATCA CCGAGTCGCG CCAGGTGGTG GAGTTGGCGG TGAAGGAACA CAAGGCCGAG 
ATTCTTGCTC TGCAGCAGGC TCTCAAGGAG CAGAAGCTCA AAGCCGAGAG CCTGTCGGAC 
AAGCTCAACG ACCTGGAGAA GAAGCACGCC ATGCTGGAGA TGAACGCCCG GAGCTTACAG 
CAGAAACTAG AGACAGAGCG GGAGCTCAAA CAGAGGCTTC TGGAGGAGCA AGCCAAATTG 
CAGCAGCAGA TGGACCTGCA GAAGAACCAC ATCTTCAGAC TGACGCAAGG GCTGCAGGAG 
GCGCTGGACC GGGCGGATCT GCTGAAGACA GAAAGGAGCG ACCTGGAGTA CCAGCTGGAA 
AACATTCAGG TTCTCTACTC TCACGAGAAA GTGAAAATGG AAGGCACAAT CTCTCAGCAA 
ACCAAACTCA TTGATTTCCT GCAAGCCAAA ATGGACCAGC CTGCTAAAAA GAAAAAGGTT 
CCTCTGCAGT ACAATGAGCT GAAGCTAGCC CTGGAAAAGG AGAAAGCCCG ATGCGCGGAG 
CTGGAGGAGG CCCTTCAGAA GACCCGCATC GAACTCCGCT CTGCGCGGGA GGAAGCTGCC 
CACCGCAAAG CCACGGACCA CCCGCACCCG TCTACGCCAG CCACTGCGAG GCAGCAGATC 
GCCATGTCTG CCATTGTGCG GTCGCCCGAG CACCAGCCCA GTGCCATGAG CCTGCTTGCC 
CCACCATCCA GCCGCAGAAA GGAGTCGTCA ACTCCAGAGG AATTCAGCCG GCGTCTGAAA 
GAGCGCATGC ACCACAACAT CCCTCACCGG TTTAATGTGG GCCTGAACAT GAGAGCCACC 
AAGTGCGCCG TGTGTCTGGA TACTGTGCAC TTTGGACGCC AGGCATCCAA ATGCCTCGAA 
TGTCAAGTGA TGTGTCACCC CAAATGCTCC ACCTGCTTGC CCGCTACCTG TGGCCTGCCA 
GCTGAATATG CCACACACTT CACTGAGGCC TTCTGCCGGG ACAAAATGAA CTCCCCGGGG 
CTCCAGAGCA AGGAGCCTGG CAGCAGCTTG CACCTGGAAG GGTGGATGAA AGTGCCCAGA 
AATAACAAAC GGGGACAGCA AGGCTGGGAC AGGAAGTACA TTGTTCTGGA GGGGTCAAAA 
GTCCTCATCT ATGACAATGA AGCCAGAGAA GCTGGACAGA GGCCGGTGGA AGAATTTGAG 
CTGTGCCTTC CTGACGGGGA CGTATCTATT CATGGTGCCG TTGGTGCTTC AGAACTTGCA 
AATACCGCCA AAGCAGATGT CCCATACATC CTGAAGATGG AGTCTCACCC ACACACCACC 
TGCTGGCCTG GGAGAACCCT CTACTTGCTA GCACCCAGCT TCCCCGACAA GCAGCGCTGG 
GTCACCGCCT TAGAATCTGT CGTCGCAGGT GGGAGAGTTT CTAGGGAAAA GGCCGAAGCC 
GATGCTAAAT TACTTGGAAA CTCTCTGCTG AAACTGGAGG GCGATGACCG GCTTGACATG 
AACTGCACCC TGCCCTTCAG TGACCAGGTA GTGCTGGTGG GCACGGAGGA AGGCCTCTAC 
GCCCTGAATG TCTTGAAAAA CTCCTTAACC CACATCCCAG GGATTGGCGC AGTCTTCCAA 
ATTTACATCA TCAAGGACCT GGAGAAGCTG CTCATGATAG CAGGGGAAGA GCGGGCTCTG 
TGCCTGGTGG ACGTGAAGAA GGTGAAGCAG TCCCTGGCGC AGTCACACCT GCCTGCCCAG 
CCCGACGTCT CCCCCAACAT ATTCGAAGCC GTCAAAGGCT GCCACTTGTT CGCTGCTGGC 
AAGATCGAGA ACAGCCTGTG CATCTGCGCC GCTATGCCAA GCAAAGTCGT CATCCTCCGC 
TACAATGACA ACCTCAGCAA GTACTGCATC CGCAAGGAGA TCGAGACCTC AGAGCCCTGC 
AGCTGTATCC ACTTCACCAA CTACAGCATC CTCATTGGGA CCAACAAATT CTATGAGATC 
GACATGAAGC AGTACACGCT TGACGAGTTC CTGGACAAGA ACGACCATTC CTTGGCACCT 
GCTGTGTTCG CCTCCTCGTC CAACAGCTTC CCTGTCTCCA TTGTGCAGGC GAACAGCGCC 
GGGCAGCGAG AAGAATACCT GCTGTGCTTC CACGAATTTG GGGTGTTCGT GGATTCTTAC 
GGAAGACGTA GCCGCACAGA TGATCTCAAG TGGAGTCGCT TACCTCTGGC CTTCGCCTAC 
AGAGAACCTT ATCTGTTTGT GACTCACTTC AACTCCCTGG AAGTCATTGA GATCCAGGCC 
AGATCCTCAC TGGGGAGCCC TGCCCGAGCA TATCTGGAAA TTCCAAACCC TCGCTACCTG 
GGCCCCGCGA TTTCCTCCGG AGCGATTTAC CTGGCCTCCT CATACCAGGA CAAGTTAAGG 
GTCATATGCT GCAAAGGAAA CCTCGTGAAG GAGTCAGGCA CTGAGCAGCA CCGGGTGCCC 
TCCACCTCCC GCAGCAGCCC CAACAAGCGA GGCCCACCAA CATACAACGA GCACATCACC 
AAACGCGTGG CCTCCAGCCC GGCGCCACCG GAAGGCCCCA GCCACCCCCG AGAGCCAAGC 
ACACCGCACC GCTACCGAGA CAGAGAGGGT CGGACAGAGC TGCGCAGGGA CAAGTCTCCA 
GGCCGCCCTC TGGAGCGGGA GAAGTCCCCA GGCCGAATGC TCAGCACTAG GAGAGAGCGG 
TCCCCAGGGA GACTGTTTGA AGACAGCAGC AGGGGCCGGC TGCCTGCAGG AGCAGTGAGG 
ACCCCACTGT CCCAGGTTAA CAAGGTCTGG GACCAGTCTT CAGTATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006530204.1
CDS	157..6324
Translation	MLKFKYGVRNPPEASASEPIASRASRLNLFFQGKPPLMTQQQMSALSREGMLDALFALFEECSQPALMKMKHVSSFVQKYSDTIAELRELQPSARDFEVRSLVGCGHFAEVQVVREKATGDVYAMKIMKKKALLAQEQVSFFEEERNILSRSTSPWIPQLQYAFQDKNNLYLVMEYQPGGDLLSLLNRYEDQLDESMIQFYLAELILAVHSVHQMGYVHRDIKPENILIDRTGHIKLVDFGSAAKMNSNKVDAKLPIGTPDYMAPEVLTVMNEDRRGTYGLDCDWWSVGVVAYEMVYGKTPFTEGTSARTFNNIMNFQRFLKFPDDPKVSSELLDLLQSLLCVQKERLKFEGLCCHPFFARTDWNNIRNSPPPFVPTLKSDDDTSNFDEPEKNSWVSSSVCQLSPSGFSGEELPFVGFSYSKALGYLGRSESVVSSLDSPAKVSSMEKKLLIKSKELQDSQDKCHKMEQEMTRLHRRVSEVEAVLSQKEVELKASETQRSLLEQDLATYITECSSLKRSLEQARMEVSQEDDKALQLLHDIREQSRKLQEIKEQEYQAQVEEMRLMMNQLEEDLVSARRRSDLYESELRESRLAAEEFKRKANECQHKLMKAKDQGKPEVGEYSKLEKINAEQQLKIQELQEKLEKAVKASTEATELLQNIRQAKERAERELEKLHNREDSSEGIKKKLVEAEERRHSLENKVKRLETMERRENRLKDDIQTKSEQIQQMADKILELEEKHREAQVSAQHLEVHLKQKEQHYEEKIKVLDNQIKKDLADKESLENMMQRHEEEAHEKGKILSEQKAMINAMDSKIRSLEQRIVELSEANKLAANSSLFTQRNMKAQEEMISELRQQKFYLETQAGKLEAQNRKLEEQLEKISHQDHSDKSRLLELETRLREVSLEHEEQKLELKRQLTELQLSLQERESQLTALQAARAALESQLRQAKTELEETTAEAEEEIQALTAHRDEIQRKFDALRNSCTVITDLEEQLNQLTEDNAELNNQNFYLSKQLDEASGANDEIVQLRSEVDHLRREITEREMQLTSQKQTMEALKTTCTMLEEQVLDLEALNDELLEKERQWEAWRSVLGDEKSQFECRVRELQRMLDTEKQSRARADQRITESRQVVELAVKEHKAEILALQQALKEQKLKAESLSDKLNDLEKKHAMLEMNARSLQQKLETERELKQRLLEEQAKLQQQMDLQKNHIFRLTQGLQEALDRADLLKTERSDLEYQLENIQVLYSHEKVKMEGTISQQTKLIDFLQAKMDQPAKKKKVPLQYNELKLALEKEKARCAELEEALQKTRIELRSAREEAAHRKATDHPHPSTPATARQQIAMSAIVRSPEHQPSAMSLLAPPSSRRKESSTPEEFSRRLKERMHHNIPHRFNVGLNMRATKCAVCLDTVHFGRQASKCLECQVMCHPKCSTCLPATCGLPAEYATHFTEAFCRDKMNSPGLQSKEPGSSLHLEGWMKVPRNNKRGQQGWDRKYIVLEGSKVLIYDNEAREAGQRPVEEFELCLPDGDVSIHGAVGASELANTAKADVPYILKMESHPHTTCWPGRTLYLLAPSFPDKQRWVTALESVVAGGRVSREKAEADAKLLGNSLLKLEGDDRLDMNCTLPFSDQVVLVGTEEGLYALNVLKNSLTHIPGIGAVFQIYIIKDLEKLLMIAGEERALCLVDVKKVKQSLAQSHLPAQPDVSPNIFEAVKGCHLFAAGKIENSLCICAAMPSKVVILRYNDNLSKYCIRKEIETSEPCSCIHFTNYSILIGTNKFYEIDMKQYTLDEFLDKNDHSLAPAVFASSSNSFPVSIVQANSAGQREEYLLCFHEFGVFVDSYGRRSRTDDLKWSRLPLAFAYREPYLFVTHFNSLEVIEIQARSSLGSPARAYLEIPNPRYLGPAISSGAIYLASSYQDKLRVICCKGNLVKESGTEQHRVPSTSRSSPNKRGPPTYNEHITKRVASSPAPPEGPSHPREPSTPHRYRDREGRTELRRDKSPGRPLEREKSPGRMLSTRRERSPGRLFEDSSRGRLPAGAVRTPLSQVNKVWDQSSV

Target ORF information:

RefSeq Version	XM_006530141.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus citron (Cit), transcript variant X12, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006530141.3

ORF Insert Sequence:

ATGTTGAAGT TCAAGTATGG TGTGCGGAAC CCGCCGGAGG CCAGTGCCTC CGAGCCCATT 
GCCAGTCGGG CCTCCAGGCT AAATCTCTTC TTCCAGGGGA AACCGCCCCT CATGACTCAA 
CAGCAGATGT CTGCTCTTTC CCGGGAAGGG ATGCTAGACG CCCTCTTCGC TCTCTTTGAA 
GAGTGCAGCC AACCCGCCCT GATGAAGATG AAGCACGTGA GCAGCTTTGT CCAGAAGTAT 
TCCGACACCA TAGCCGAGTT GCGGGAGCTG CAGCCGTCGG CGAGAGACTT CGAAGTTCGA 
AGCCTTGTGG GCTGTGGTCA CTTCGCTGAA GTGCAGGTGG TTAGAGAGAA GGCGACCGGG 
GACGTCTATG CCATGAAAAT CATGAAGAAG AAGGCTTTGC TGGCCCAGGA ACAGGTTTCA 
TTTTTCGAGG AGGAGAGGAA CATATTATCT CGGAGCACGA GTCCTTGGAT CCCCCAGTTA 
CAGTACGCCT TTCAGGACAA AAATAACCTT TACCTGGTCA TGGAATATCA GCCTGGAGGG 
GATTTGCTGT CGCTTCTGAA CAGATACGAG GACCAATTAG ATGAGAGCAT GATCCAGTTT 
TACCTTGCTG AGCTGATTTT GGCTGTCCAC AGCGTGCACC AGATGGGATA TGTGCATCGA 
GACATCAAGC CCGAGAACAT CCTCATCGAC CGGACGGGAC ACATCAAGCT GGTGGATTTT 
GGATCAGCCG CTAAGATGAA TTCAAATAAG GTGGATGCCA AACTCCCCAT TGGGACCCCG 
GATTACATGG CTCCGGAAGT GTTGACCGTG ATGAACGAGG ACCGAAGGGG CACATACGGC 
TTGGACTGTG ACTGGTGGTC TGTCGGAGTT GTTGCTTATG AGATGGTTTA TGGGAAGACC 
CCATTCACAG AGGGAACCTC CGCCCGGACC TTCAACAACA TCATGAACTT CCAGCGGTTT 
TTGAAGTTCC CAGATGACCC CAAAGTTAGC AGTGAGCTCC TTGATCTGCT TCAGAGTCTG 
CTGTGTGTCC AGAAAGAGAG ACTGAAGTTC GAGGGTCTCT GCTGCCACCC TTTCTTTGCC 
AGAACGGACT GGAACAACAT CCGTAACTCT CCTCCCCCCT TCGTCCCCAC CCTCAAGTCT 
GACGATGACA CCTCCAATTT TGATGAACCA GAGAAGAATT CGTGGGTTTC ATCCTCTGTG 
TGCCAGCTGA GCCCCTCGGG CTTCTCAGGC GAAGAGCTGC CGTTTGTGGG ATTTTCGTAC 
AGCAAGGCAC TGGGGTATCT TGGTAGATCT GAGTCTGTCG TGTCGAGTCT GGACTCCCCT 
GCCAAGGTTA GCTCCATGGA AAAGAAACTT CTCATCAAAA GCAAAGAGCT CCAAGACTCC 
CAGGACAAGT GTCACAAGAT GGAGCAGGAA ATGACCCGGT TACATCGCAG AGTGTCAGAG 
GTGGAGGCTG TGCTTAGTCA GAAGGAGGTG GAGCTGAAGG CCTCTGAGAC TCAGAGATCC 
CTCCTGGAGC AGGACCTTGC TACCTACATC ACAGAATGCA GTAGCTTAAA GCGAAGTTTG 
GAGCAAGCGC GGATGGAGGT GTCCCAGGAG GATGACAAAG CTCTGCAGCT TCTCCACGAC 
ATCCGAGAGC AGAGCCGGAA GCTCCAGGAG ATCAAGGAGC AGGAGTACCA GGCTCAGGTG 
GAGGAGATGA GGCTGATGAT GAATCAGCTG GAAGAAGACC TGGTGTCAGC CCGCAGACGC 
AGCGATCTCT ACGAGTCTGA GCTGAGGGAG TCTCGGCTTG CCGCCGAGGA ATTCAAGCGG 
AAGGCAAACG AATGTCAGCA CAAACTGATG AAGGCTAAGG ACCAAGGGAA GCCTGAAGTG 
GGAGAATATT CCAAACTGGA GAAGATCAAT GCTGAGCAGC AGCTGAAGAT CCAGGAGCTC 
CAGGAGAAGC TGGAGAAGGC TGTAAAAGCC AGCACAGAGG CCACCGAGCT CCTGCAGAAT 
ATCCGCCAGG CAAAGGAGCG AGCAGAGCGG GAGCTAGAGA AGCTACACAA CCGGGAAGAC 
TCCTCCGAGG GCATCAAAAA GAAGCTGGTG GAAGCCGAGG AACGCCGCCA CTCCCTGGAG 
AACAAGGTAA AGAGACTAGA GACCATGGAG CGTAGAGAGA ACAGACTGAA GGATGACATC 
CAGACAAAGT CCGAACAGAT CCAGCAGATG GCTGATAAAA TTCTGGAGCT GGAGGAGAAA 
CATCGGGAGG CTCAGGTCTC AGCTCAACAT CTAGAAGTAC ACTTGAAACA GAAAGAACAG 
CACTACGAGG AAAAGATCAA GGTATTGGAC AATCAGATAA AGAAGGACCT GGCCGACAAG 
GAGAGCCTGG AGAACATGAT GCAGAGACAC GAGGAGGAGG CCCACGAGAA GGGCAAGATC 
CTCAGCGAGC AGAAGGCGAT GATCAACGCG ATGGATTCCA AGATCCGATC CCTGGAGCAG 
AGGATCGTGG AGCTGTCGGA AGCCAACAAG CTTGCGGCAA ACAGCAGTCT CTTCACCCAG 
AGGAACATGA AGGCCCAGGA AGAGATGATC TCAGAACTCA GGCAGCAGAA ATTTTACCTG 
GAGACGCAGG CCGGAAAGCT GGAGGCCCAG AACCGAAAGC TGGAAGAGCA ACTGGAGAAG 
ATCAGCCACC AAGATCACAG TGACAAGAGT CGGCTGCTGG AGCTGGAAAC AAGGCTGAGG 
GAGGTCAGCC TGGAGCACGA GGAGCAGAAG CTGGAGCTGA AGCGGCAGCT CACGGAGCTG 
CAGCTGTCCC TGCAGGAGCG CGAGTCCCAG CTGACGGCCC TGCAGGCCGC CCGGGCAGCG 
CTGGAGAGCC AGCTCCGCCA GGCGAAGACA GAGCTGGAGG AGACAACCGC GGAAGCGGAG 
GAGGAGATCC AGGCGCTCAC GGCACATCGA GATGAGATCC AGCGCAAATT CGATGCCCTT 
CGCAACAGCT GCACGGTCAT CACCGACCTG GAGGAGCAGC TGAACCAGCT CACCGAGGAC 
AACGCCGAGC TCAACAACCA AAACTTCTAC CTGTCCAAAC AACTCGATGA GGCTTCCGGG 
GCCAATGACG AGATTGTGCA GCTGCGGAGT GAGGTGGACC ATCTCCGCCG TGAGATCACG 
GAGCGGGAGA TGCAGCTCAC CAGCCAGAAG CAAACGATGG AGGCTCTGAA GACGACATGC 
ACCATGCTGG AGGAGCAGGT CCTGGACCTG GAGGCCCTGA ACGACGAGCT GCTGGAGAAG 
GAGCGCCAGT GGGAGGCCTG GCGGAGCGTC CTCGGCGACG AGAAGTCCCA GTTCGAGTGT 
CGAGTTCGAG AGCTACAGAG GATGCTGGAC ACCGAGAAGC AGAGCAGGGC TAGGGCCGAT 
CAGCGGATCA CCGAGTCGCG CCAGGTGGTG GAGTTGGCGG TGAAGGAACA CAAGGCCGAG 
ATTCTTGCTC TGCAGCAGGC TCTCAAGGAG CAGAAGCTCA AAGCCGAGAG CCTGTCGGAC 
AAGCTCAACG ACCTGGAGAA GAAGCACGCC ATGCTGGAGA TGAACGCCCG GAGCTTACAG 
CAGAAACTAG AGACAGAGCG GGAGCTCAAA CAGAGGCTTC TGGAGGAGCA AGCCAAATTG 
CAGCAGCAGA TGGACCTGCA GAAGAACCAC ATCTTCAGAC TGACGCAAGG GCTGCAGGAG 
GCGCTGGACC GGGCGGATCT GCTGAAGACA GAAAGGAGCG ACCTGGAGTA CCAGCTGGAA 
AACATTCAGG TTCTCTACTC TCACGAGAAA GTGAAAATGG AAGGCACAAT CTCTCAGCAA 
ACCAAACTCA TTGATTTCCT GCAAGCCAAA ATGGACCAGC CTGCTAAAAA GAAAAAGGTT 
CCTCTGCAGT ACAATGAGCT GAAGCTAGCC CTGGAAAAGG AGAAAGCCCG ATGCGCGGAG 
CTGGAGGAGG CCCTTCAGAA GACCCGCATC GAACTCCGCT CTGCGCGGGA GGAAGCTGCC 
CACCGCAAAG CCACGGACCA CCCGCACCCG TCTACGCCAG CCACTGCGAG GCAGCAGATC 
GCCATGTCTG CCATTGTGCG GTCGCCCGAG CACCAGCCCA GTGCCATGAG CCTGCTTGCC 
CCACCATCCA GCCGCAGAAA GGAGTCGTCA ACTCCAGAGG AATTCAGCCG GCGTCTGAAA 
GAGCGCATGC ACCACAACAT CCCTCACCGG TTTAATGTGG GCCTGAACAT GAGAGCCACC 
AAGTGCGCCG TGTGTCTGGA TACTGTGCAC TTTGGACGCC AGGCATCCAA ATGCCTCGAA 
TGTCAAGTGA TGTGTCACCC CAAATGCTCC ACCTGCTTGC CCGCTACCTG TGGCCTGCCA 
GCTGAATATG CCACACACTT CACTGAGGCC TTCTGCCGGG ACAAAATGAA CTCCCCGGGG 
CTCCAGAGCA AGGAGCCTGG CAGCAGCTTG CACCTGGAAG GGTGGATGAA AGTGCCCAGA 
AATAACAAAC GGGGACAGCA AGGCTGGGAC AGGAAGTACA TTGTTCTGGA GGGGTCAAAA 
GTCCTCATCT ATGACAATGA AGCCAGAGAA GCTGGACAGA GGCCGGTGGA AGAATTTGAG 
CTGTGCCTTC CTGACGGGGA CGTATCTATT CATGGTGCCG TTGGTGCTTC AGAACTTGCA 
AATACCGCCA AAGCAGATGT CCCATACATC CTGAAGATGG AGTCTCACCC ACACACCACC 
TGCTGGCCTG GGAGAACCCT CTACTTGCTA GCACCCAGCT TCCCCGACAA GCAGCGCTGG 
GTCACCGCCT TAGAATCTGT CGTCGCAGGT GGGAGAGTTT CTAGGGAAAA GGCCGAAGCC 
GATGCTAAAT TACTTGGAAA CTCTCTGCTG AAACTGGAGG GCGATGACCG GCTTGACATG 
AACTGCACCC TGCCCTTCAG TGACCAGGTA GTGCTGGTGG GCACGGAGGA AGGCCTCTAC 
GCCCTGAATG TCTTGAAAAA CTCCTTAACC CACATCCCAG GGATTGGCGC AGTCTTCCAA 
ATTTACATCA TCAAGGACCT GGAGAAGCTG CTCATGATAG CAGGGGAAGA GCGGGCTCTG 
TGCCTGGTGG ACGTGAAGAA GGTGAAGCAG TCCCTGGCGC AGTCACACCT GCCTGCCCAG 
CCCGACGTCT CCCCCAACAT ATTCGAAGCC GTCAAAGGCT GCCACTTGTT CGCTGCTGGC 
AAGATCGAGA ACAGCCTGTG CATCTGCGCC GCTATGCCAA GCAAAGTCGT CATCCTCCGC 
TACAATGACA ACCTCAGCAA GTACTGCATC CGCAAGGAGA TCGAGACCTC AGAGCCCTGC 
AGCTGTATCC ACTTCACCAA CTACAGCATC CTCATTGGGA CCAACAAATT CTATGAGATC 
GACATGAAGC AGTACACGCT TGACGAGTTC CTGGACAAGA ACGACCATTC CTTGGCACCT 
GCTGTGTTCG CCTCCTCGTC CAACAGCTTC CCTGTCTCCA TTGTGCAGGC GAACAGCGCC 
GGGCAGCGAG AAGAATACCT GCTGTGCTTC CACGAATTTG GGGTGTTCGT GGATTCTTAC 
GGAAGACGTA GCCGCACAGA TGATCTCAAG TGGAGTCGCT TACCTCTGGC CTTCGCCTAC 
AGAGAACCTT ATCTGTTTGT GACTCACTTC AACTCCCTGG AAGTCATTGA GATCCAGGCC 
AGATCCTCAC TGGGGAGCCC TGCCCGAGCA TATCTGGAAA TTCCAAACCC TCGCTACCTG 
GGCCCCGCGA TTTCCTCCGG AGCGATTTAC CTGGCCTCCT CATACCAGGA CAAGTTAAGG 
GTCATATGCT GCAAAGGAAA CCTCGTGAAG GAGTCAGGCA CTGAGCAGCA CCGGGTGCCC 
TCCACCTCCC GCAGCAGCCC CAACAAGCGA GGCCCACCAA CATACAACGA GCACATCACC 
AAACGCGTGG CCTCCAGCCC GGCGCCACCG GAAGGCCCCA GCCACCCCCG AGAGCCAAGC 
ACACCGCACC GCTACCGAGA CAGAGAGGGT CGGACAGAGC TGCGCAGGGA CAAGTCTCCA 
GGCCGCCCTC TGGAGCGGGA GAAGTCCCCA GGCCGAATGC TCAGCACTAG GAGAGAGCGG 
TCCCCAGGGA GACTGTTTGA AGACAGCAGC AGGGGCCGGC TGCCTGCAGG AGCAGTGAGG 
ACCCCACTGT CCCAGGTTAA CAAGGTCTGG GACCAGTCTT CAGTATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cit ( XM_006530130.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006530193.1 Mus musculus citron (Cit), transcript variant X1, mRNA.

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CloneID	OMu35774
Clone ID Related Accession (Same CDS sequence)	XM_006530130.3 , XM_006530132.3 , XM_006530133.3 , XM_006530130.4 , XM_006530131.2 , XM_006530131.1 , XM_006530133.4 , XM_006530132.4
Accession Version	XM_006530130.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6372bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	citron Rho-interacting kinase isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000071.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006530130.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTGAAGT TCAAGTATGG TGTGCGGAAC CCGCCGGAGG CCAGTGCCTC CGAGCCCATT 
GCCAGTCGGG CCTCCAGGCT AAATCTCTTC TTCCAGGGGA AACCGCCCCT CATGACTCAA 
CAGCAGATGT CTGCTCTTTC CCGGGAAGGG ATGCTAGACG CCCTCTTCGC TCTCTTTGAA 
GAGTGCAGCC AACCCGCCCT GATGAAGATG AAGCACGTGA GCAGCTTTGT CCAGAAGTAT 
TCCGACACCA TAGCCGAGTT GCGGGAGCTG CAGCCGTCGG CGAGAGACTT CGAAGTTCGA 
AGCCTTGTGG GCTGTGGTCA CTTCGCTGAA GTGCAGGTGG TTAGAGAGAA GGCGACCGGG 
GACGTCTATG CCATGAAAAT CATGAAGAAG AAGGCTTTGC TGGCCCAGGA ACAGGTTTCA 
TTTTTCGAGG AGGAGAGGAA CATATTATCT CGGAGCACGA GTCCTTGGAT CCCCCAGTTA 
CAGTACGCCT TTCAGGACAA AAATAACCTT TACCTGGTCA TGGAATATCA GCCTGGAGGG 
GATTTGCTGT CGCTTCTGAA CAGATACGAG GACCAATTAG ATGAGAGCAT GATCCAGTTT 
TACCTTGCTG AGCTGATTTT GGCTGTCCAC AGCGTGCACC AGATGGGATA TGTGCATCGA 
GACATCAAGC CCGAGAACAT CCTCATCGAC CGGACGGGAC ACATCAAGCT GGTGGATTTT 
GGATCAGCCG CTAAGATGAA TTCAAATAAG GTGGATGCCA AACTCCCCAT TGGGACCCCG 
GATTACATGG CTCCGGAAGT GTTGACCGTG ATGAACGAGG ACCGAAGGGG CACATACGGC 
TTGGACTGTG ACTGGTGGTC TGTCGGAGTT GTTGCTTATG AGATGGTTTA TGGGAAGACC 
CCATTCACAG AGGGAACCTC CGCCCGGACC TTCAACAACA TCATGAACTT CCAGCGGTTT 
TTGAAGTTCC CAGATGACCC CAAAGTTAGC AGTGAGCTCC TTGATCTGCT TCAGAGTCTG 
CTGTGTGTCC AGAAAGAGAG ACTGAAGTTC GAGGGTCTCT GCTGCCACCC TTTCTTTGCC 
AGAACGGACT GGAACAACAT CCGTAACTCT CCTCCCCCCT TCGTCCCCAC CCTCAAGTCT 
GACGATGACA CCTCCAATTT TGATGAACCA GAGAAGAATT CGTGGGTTTC ATCCTCTGTG 
TGCCAGCTGA GCCCCTCGGG CTTCTCAGGC GAAGAGCTGC CGTTTGTGGG ATTTTCGTAC 
AGCAAGGCAC TGGGGTATCT TGGTAGATCT GAGTCTGTCG TGTCGAGTCT GGACTCCCCT 
GCCAAGGTTA GCTCCATGGA AAAGAAACTT CTCATCAAAA GCAAAGAGCT CCAAGACTCC 
CAGGACAAGT GTCACAAGAT GGAGCAGGAA ATGACCCGGT TACATCGCAG AGTGTCAGAG 
GTGGAGGCTG TGCTTAGTCA GAAGGAGGTG GAGCTGAAGG CCTCTGAGAC TCAGAGATCC 
CTCCTGGAGC AGGACCTTGC TACCTACATC ACAGAATGCA GTAGCTTAAA GCGAAGTTTG 
GAGCAAGCGC GGATGGAGGT GTCCCAGGAG GATGACAAAG CTCTGCAGCT TCTCCACGAC 
ATCCGAGAGC AGAGCCGGAA GCTCCAGGAG ATCAAGGAGC AGGAGTACCA GGCTCAGGTG 
GAGGAGATGA GGCTGATGAT GAATCAGCTG GAAGAAGACC TGGTGTCAGC CCGCAGACGC 
AGCGATCTCT ACGAGTCTGA GCTGAGGGAG TCTCGGCTTG CCGCCGAGGA ATTCAAGCGG 
AAGGCAAACG AATGTCAGCA CAAACTGATG AAGGTAGTCA GCCACCCTCC AAGAGGGGAC 
TCCGGGGGCA CGGCCCTGGA TGATCTTCAC AAGATGCAAG GCCACGCTGG CTTAACGAGT 
GCTAAGGACC AAGGGAAGCC TGAAGTGGGA GAATATTCCA AACTGGAGAA GATCAATGCT 
GAGCAGCAGC TGAAGATCCA GGAGCTCCAG GAGAAGCTGG AGAAGGCTGT AAAAGCCAGC 
ACAGAGGCCA CCGAGCTCCT GCAGAATATC CGCCAGGCAA AGGAGCGAGC AGAGCGGGAG 
CTAGAGAAGC TACACAACCG GGAAGACTCC TCCGAGGGCA TCAAAAAGAA GCTGGTGGAA 
GCCGAGGAAC GCCGCCACTC CCTGGAGAAC AAGGTAAAGA GACTAGAGAC CATGGAGCGT 
AGAGAGAACA GACTGAAGGA TGACATCCAG ACAAAGTCCG AACAGATCCA GCAGATGGCT 
GATAAAATTC TGGAGCTGGA GGAGAAACAT CGGGAGGCTC AGGTCTCAGC TCAACATCTA 
GAAGTACACT TGAAACAGAA AGAACAGCAC TACGAGGAAA AGATCAAGGT ATTGGACAAT 
CAGATAAAGA AGGACCTGGC CGACAAGGAG AGCCTGGAGA ACATGATGCA GAGACACGAG 
GAGGAGGCCC ACGAGAAGGG CAAGATCCTC AGCGAGCAGA AGGCGATGAT CAACGCGATG 
GATTCCAAGA TCCGATCCCT GGAGCAGAGG ATCGTGGAGC TGTCGGAAGC CAACAAGCTT 
GCGGCAAACA GCAGTCTCTT CACCCAGAGG AACATGAAGG CCCAGGAAGA GATGATCTCA 
GAACTCAGGC AGCAGAAATT TTACCTGGAG ACGCAGGCCG GAAAGCTGGA GGCCCAGAAC 
CGAAAGCTGG AAGAGCAACT GGAGAAGATC AGCCACCAAG ATCACAGTGA CAAGAGTCGG 
CTGCTGGAGC TGGAAACAAG GCTGAGGGAG GTCAGCCTGG AGCACGAGGA GCAGAAGCTG 
GAGCTGAAGC GGCAGCTCAC GGAGCTGCAG CTGTCCCTGC AGGAGCGCGA GTCCCAGCTG 
ACGGCCCTGC AGGCCGCCCG GGCAGCGCTG GAGAGCCAGC TCCGCCAGGC GAAGACAGAG 
CTGGAGGAGA CAACCGCGGA AGCGGAGGAG GAGATCCAGG CGCTCACGGC ACATCGAGAT 
GAGATCCAGC GCAAATTCGA TGCCCTTCGC AACAGCTGCA CGGTCATCAC CGACCTGGAG 
GAGCAGCTGA ACCAGCTCAC CGAGGACAAC GCCGAGCTCA ACAACCAAAA CTTCTACCTG 
TCCAAACAAC TCGATGAGGC TTCCGGGGCC AATGACGAGA TTGTGCAGCT GCGGAGTGAG 
GTGGACCATC TCCGCCGTGA GATCACGGAG CGGGAGATGC AGCTCACCAG CCAGAAGCAA 
GCACAACTCA GTGCTCCTGA TTTGCAGACG ATGGAGGCTC TGAAGACGAC ATGCACCATG 
CTGGAGGAGC AGGTCCTGGA CCTGGAGGCC CTGAACGACG AGCTGCTGGA GAAGGAGCGC 
CAGTGGGAGG CCTGGCGGAG CGTCCTCGGC GACGAGAAGT CCCAGTTCGA GTGTCGAGTT 
CGAGAGCTAC AGAGGATGCT GGACACCGAG AAGCAGAGCA GGGCTAGGGC CGATCAGCGG 
ATCACCGAGT CGCGCCAGGT GGTGGAGTTG GCGGTGAAGG AACACAAGGC CGAGATTCTT 
GCTCTGCAGC AGGCTCTCAA GGAGCAGAAG CTCAAAGCCG AGAGCCTGTC GGACAAGCTC 
AACGACCTGG AGAAGAAGCA CGCCATGCTG GAGATGAACG CCCGGAGCTT ACAGCAGAAA 
CTAGAGACAG AGCGGGAGCT CAAACAGAGG CTTCTGGAGG AGCAAGCCAA ATTGCAGCAG 
CAGATGGACC TGCAGAAGAA CCACATCTTC AGACTGACGC AAGGGCTGCA GGAGGCGCTG 
GACCGGGCGG ATCTGCTGAA GACAGAAAGG AGCGACCTGG AGTACCAGCT GGAAAACATT 
CAGGTTCTCT ACTCTCACGA GAAAGTGAAA ATGGAAGGCA CAATCTCTCA GCAAACCAAA 
CTCATTGATT TCCTGCAAGC CAAAATGGAC CAGCCTGCTA AAAAGAAAAA GGGTTTATTT 
AGTCGACGGA AAGAGGACCC TGCTTTGCCC ACACAGGTTC CTCTGCAGTA CAATGAGCTG 
AAGCTAGCCC TGGAAAAGGA GAAAGCCCGA TGCGCGGAGC TGGAGGAGGC CCTTCAGAAG 
ACCCGCATCG AACTCCGCTC TGCGCGGGAG GAAGCTGCCC ACCGCAAAGC CACGGACCAC 
CCGCACCCGT CTACGCCAGC CACTGCGAGG CAGCAGATCG CCATGTCTGC CATTGTGCGG 
TCGCCCGAGC ACCAGCCCAG TGCCATGAGC CTGCTTGCCC CACCATCCAG CCGCAGAAAG 
GAGTCGTCAA CTCCAGAGGA ATTCAGCCGG CGTCTGAAAG AGCGCATGCA CCACAACATC 
CCTCACCGGT TTAATGTGGG CCTGAACATG AGAGCCACCA AGTGCGCCGT GTGTCTGGAT 
ACTGTGCACT TTGGACGCCA GGCATCCAAA TGCCTCGAAT GTCAAGTGAT GTGTCACCCC 
AAATGCTCCA CCTGCTTGCC CGCTACCTGT GGCCTGCCAG CTGAATATGC CACACACTTC 
ACTGAGGCCT TCTGCCGGGA CAAAATGAAC TCCCCGGGGC TCCAGAGCAA GGAGCCTGGC 
AGCAGCTTGC ACCTGGAAGG GTGGATGAAA GTGCCCAGAA ATAACAAACG GGGACAGCAA 
GGCTGGGACA GGAAGTACAT TGTTCTGGAG GGGTCAAAAG TCCTCATCTA TGACAATGAA 
GCCAGAGAAG CTGGACAGAG GCCGGTGGAA GAATTTGAGC TGTGCCTTCC TGACGGGGAC 
GTATCTATTC ATGGTGCCGT TGGTGCTTCA GAACTTGCAA ATACCGCCAA AGCAGATGTC 
CCATACATCC TGAAGATGGA GTCTCACCCA CACACCACCT GCTGGCCTGG GAGAACCCTC 
TACTTGCTAG CACCCAGCTT CCCCGACAAG CAGCGCTGGG TCACCGCCTT AGAATCTGTC 
GTCGCAGGTG GGAGAGTTTC TAGGGAAAAG GCCGAAGCCG ATGCTGCACG TGACCATACT 
TCGAGTGAGC ATCAGCCAGT GTGGGTTGAG AAATTACTTG GAAACTCTCT GCTGAAACTG 
GAGGGCGATG ACCGGCTTGA CATGAACTGC ACCCTGCCCT TCAGTGACCA GGTAGTGCTG 
GTGGGCACGG AGGAAGGCCT CTACGCCCTG AATGTCTTGA AAAACTCCTT AACCCACATC 
CCAGGGATTG GCGCAGTCTT CCAAATTTAC ATCATCAAGG ACCTGGAGAA GCTGCTCATG 
ATAGCAGGGG AAGAGCGGGC TCTGTGCCTG GTGGACGTGA AGAAGGTGAA GCAGTCCCTG 
GCGCAGTCAC ACCTGCCTGC CCAGCCCGAC GTCTCCCCCA ACATATTCGA AGCCGTCAAA 
GGCTGCCACT TGTTCGCTGC TGGCAAGATC GAGAACAGCC TGTGCATCTG CGCCGCTATG 
CCAAGCAAAG TCGTCATCCT CCGCTACAAT GACAACCTCA GCAAGTACTG CATCCGCAAG 
GAGATCGAGA CCTCAGAGCC CTGCAGCTGT ATCCACTTCA CCAACTACAG CATCCTCATT 
GGGACCAACA AATTCTATGA GATCGACATG AAGCAGTACA CGCTTGACGA GTTCCTGGAC 
AAGAACGACC ATTCCTTGGC ACCTGCTGTG TTCGCCTCCT CGTCCAACAG CTTCCCTGTC 
TCCATTGTGC AGGCGAACAG CGCCGGGCAG CGAGAAGAAT ACCTGCTGTG CTTCCACGAA 
TTTGGGGTGT TCGTGGATTC TTACGGAAGA CGTAGCCGCA CAGATGATCT CAAGTGGAGT 
CGCTTACCTC TGGCCTTCGC CTACAGAGAA CCTTATCTGT TTGTGACTCA CTTCAACTCC 
CTGGAAGTCA TTGAGATCCA GGCCAGATCC TCACTGGGGA GCCCTGCCCG AGCATATCTG 
GAAATTCCAA ACCCTCGCTA CCTGGGCCCC GCGATTTCCT CCGGAGCGAT TTACCTGGCC 
TCCTCATACC AGGACAAGTT AAGGGTCATA TGCTGCAAAG GAAACCTCGT GAAGGAGTCA 
GGCACTGAGC AGCACCGGGT GCCCTCCACC TCCCGCAGCA GCCCCAACAA GCGAGGCCCA 
CCAACATACA ACGAGCACAT CACCAAACGC GTGGCCTCCA GCCCGGCGCC ACCGGAAGGC 
CCCAGCCACC CCCGAGAGCC AAGCACACCG CACCGCTACC GAGACAGAGA GGGTCGGACA 
GAGCTGCGCA GGGACAAGTC TCCAGGCCGC CCTCTGGAGC GGGAGAAGTC CCCAGGCCGA 
ATGCTCAGCA CTAGGAGAGA GCGGTCCCCA GGGAGACTGT TTGAAGACAG CAGCAGGGGC 
CGGCTGCCTG CAGGAGCAGT GAGGACCCCA CTGTCCCAGG TTAACAAGGT CTGGGACCAG 
TCTTCAGTAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006530193.1
CDS	158..6529
Translation	MLKFKYGVRNPPEASASEPIASRASRLNLFFQGKPPLMTQQQMSALSREGMLDALFALFEECSQPALMKMKHVSSFVQKYSDTIAELRELQPSARDFEVRSLVGCGHFAEVQVVREKATGDVYAMKIMKKKALLAQEQVSFFEEERNILSRSTSPWIPQLQYAFQDKNNLYLVMEYQPGGDLLSLLNRYEDQLDESMIQFYLAELILAVHSVHQMGYVHRDIKPENILIDRTGHIKLVDFGSAAKMNSNKVDAKLPIGTPDYMAPEVLTVMNEDRRGTYGLDCDWWSVGVVAYEMVYGKTPFTEGTSARTFNNIMNFQRFLKFPDDPKVSSELLDLLQSLLCVQKERLKFEGLCCHPFFARTDWNNIRNSPPPFVPTLKSDDDTSNFDEPEKNSWVSSSVCQLSPSGFSGEELPFVGFSYSKALGYLGRSESVVSSLDSPAKVSSMEKKLLIKSKELQDSQDKCHKMEQEMTRLHRRVSEVEAVLSQKEVELKASETQRSLLEQDLATYITECSSLKRSLEQARMEVSQEDDKALQLLHDIREQSRKLQEIKEQEYQAQVEEMRLMMNQLEEDLVSARRRSDLYESELRESRLAAEEFKRKANECQHKLMKVVSHPPRGDSGGTALDDLHKMQGHAGLTSAKDQGKPEVGEYSKLEKINAEQQLKIQELQEKLEKAVKASTEATELLQNIRQAKERAERELEKLHNREDSSEGIKKKLVEAEERRHSLENKVKRLETMERRENRLKDDIQTKSEQIQQMADKILELEEKHREAQVSAQHLEVHLKQKEQHYEEKIKVLDNQIKKDLADKESLENMMQRHEEEAHEKGKILSEQKAMINAMDSKIRSLEQRIVELSEANKLAANSSLFTQRNMKAQEEMISELRQQKFYLETQAGKLEAQNRKLEEQLEKISHQDHSDKSRLLELETRLREVSLEHEEQKLELKRQLTELQLSLQERESQLTALQAARAALESQLRQAKTELEETTAEAEEEIQALTAHRDEIQRKFDALRNSCTVITDLEEQLNQLTEDNAELNNQNFYLSKQLDEASGANDEIVQLRSEVDHLRREITEREMQLTSQKQAQLSAPDLQTMEALKTTCTMLEEQVLDLEALNDELLEKERQWEAWRSVLGDEKSQFECRVRELQRMLDTEKQSRARADQRITESRQVVELAVKEHKAEILALQQALKEQKLKAESLSDKLNDLEKKHAMLEMNARSLQQKLETERELKQRLLEEQAKLQQQMDLQKNHIFRLTQGLQEALDRADLLKTERSDLEYQLENIQVLYSHEKVKMEGTISQQTKLIDFLQAKMDQPAKKKKGLFSRRKEDPALPTQVPLQYNELKLALEKEKARCAELEEALQKTRIELRSAREEAAHRKATDHPHPSTPATARQQIAMSAIVRSPEHQPSAMSLLAPPSSRRKESSTPEEFSRRLKERMHHNIPHRFNVGLNMRATKCAVCLDTVHFGRQASKCLECQVMCHPKCSTCLPATCGLPAEYATHFTEAFCRDKMNSPGLQSKEPGSSLHLEGWMKVPRNNKRGQQGWDRKYIVLEGSKVLIYDNEAREAGQRPVEEFELCLPDGDVSIHGAVGASELANTAKADVPYILKMESHPHTTCWPGRTLYLLAPSFPDKQRWVTALESVVAGGRVSREKAEADAARDHTSSEHQPVWVEKLLGNSLLKLEGDDRLDMNCTLPFSDQVVLVGTEEGLYALNVLKNSLTHIPGIGAVFQIYIIKDLEKLLMIAGEERALCLVDVKKVKQSLAQSHLPAQPDVSPNIFEAVKGCHLFAAGKIENSLCICAAMPSKVVILRYNDNLSKYCIRKEIETSEPCSCIHFTNYSILIGTNKFYEIDMKQYTLDEFLDKNDHSLAPAVFASSSNSFPVSIVQANSAGQREEYLLCFHEFGVFVDSYGRRSRTDDLKWSRLPLAFAYREPYLFVTHFNSLEVIEIQARSSLGSPARAYLEIPNPRYLGPAISSGAIYLASSYQDKLRVICCKGNLVKESGTEQHRVPSTSRSSPNKRGPPTYNEHITKRVASSPAPPEGPSHPREPSTPHRYRDREGRTELRRDKSPGRPLEREKSPGRMLSTRRERSPGRLFEDSSRGRLPAGAVRTPLSQVNKVWDQSSV

Target ORF information:

RefSeq Version	XM_006530130.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus citron (Cit), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006530130.3

ORF Insert Sequence:

ATGTTGAAGT TCAAGTATGG TGTGCGGAAC CCGCCGGAGG CCAGTGCCTC CGAGCCCATT 
GCCAGTCGGG CCTCCAGGCT AAATCTCTTC TTCCAGGGGA AACCGCCCCT CATGACTCAA 
CAGCAGATGT CTGCTCTTTC CCGGGAAGGG ATGCTAGACG CCCTCTTCGC TCTCTTTGAA 
GAGTGCAGCC AACCCGCCCT GATGAAGATG AAGCACGTGA GCAGCTTTGT CCAGAAGTAT 
TCCGACACCA TAGCCGAGTT GCGGGAGCTG CAGCCGTCGG CGAGAGACTT CGAAGTTCGA 
AGCCTTGTGG GCTGTGGTCA CTTCGCTGAA GTGCAGGTGG TTAGAGAGAA GGCGACCGGG 
GACGTCTATG CCATGAAAAT CATGAAGAAG AAGGCTTTGC TGGCCCAGGA ACAGGTTTCA 
TTTTTCGAGG AGGAGAGGAA CATATTATCT CGGAGCACGA GTCCTTGGAT CCCCCAGTTA 
CAGTACGCCT TTCAGGACAA AAATAACCTT TACCTGGTCA TGGAATATCA GCCTGGAGGG 
GATTTGCTGT CGCTTCTGAA CAGATACGAG GACCAATTAG ATGAGAGCAT GATCCAGTTT 
TACCTTGCTG AGCTGATTTT GGCTGTCCAC AGCGTGCACC AGATGGGATA TGTGCATCGA 
GACATCAAGC CCGAGAACAT CCTCATCGAC CGGACGGGAC ACATCAAGCT GGTGGATTTT 
GGATCAGCCG CTAAGATGAA TTCAAATAAG GTGGATGCCA AACTCCCCAT TGGGACCCCG 
GATTACATGG CTCCGGAAGT GTTGACCGTG ATGAACGAGG ACCGAAGGGG CACATACGGC 
TTGGACTGTG ACTGGTGGTC TGTCGGAGTT GTTGCTTATG AGATGGTTTA TGGGAAGACC 
CCATTCACAG AGGGAACCTC CGCCCGGACC TTCAACAACA TCATGAACTT CCAGCGGTTT 
TTGAAGTTCC CAGATGACCC CAAAGTTAGC AGTGAGCTCC TTGATCTGCT TCAGAGTCTG 
CTGTGTGTCC AGAAAGAGAG ACTGAAGTTC GAGGGTCTCT GCTGCCACCC TTTCTTTGCC 
AGAACGGACT GGAACAACAT CCGTAACTCT CCTCCCCCCT TCGTCCCCAC CCTCAAGTCT 
GACGATGACA CCTCCAATTT TGATGAACCA GAGAAGAATT CGTGGGTTTC ATCCTCTGTG 
TGCCAGCTGA GCCCCTCGGG CTTCTCAGGC GAAGAGCTGC CGTTTGTGGG ATTTTCGTAC 
AGCAAGGCAC TGGGGTATCT TGGTAGATCT GAGTCTGTCG TGTCGAGTCT GGACTCCCCT 
GCCAAGGTTA GCTCCATGGA AAAGAAACTT CTCATCAAAA GCAAAGAGCT CCAAGACTCC 
CAGGACAAGT GTCACAAGAT GGAGCAGGAA ATGACCCGGT TACATCGCAG AGTGTCAGAG 
GTGGAGGCTG TGCTTAGTCA GAAGGAGGTG GAGCTGAAGG CCTCTGAGAC TCAGAGATCC 
CTCCTGGAGC AGGACCTTGC TACCTACATC ACAGAATGCA GTAGCTTAAA GCGAAGTTTG 
GAGCAAGCGC GGATGGAGGT GTCCCAGGAG GATGACAAAG CTCTGCAGCT TCTCCACGAC 
ATCCGAGAGC AGAGCCGGAA GCTCCAGGAG ATCAAGGAGC AGGAGTACCA GGCTCAGGTG 
GAGGAGATGA GGCTGATGAT GAATCAGCTG GAAGAAGACC TGGTGTCAGC CCGCAGACGC 
AGCGATCTCT ACGAGTCTGA GCTGAGGGAG TCTCGGCTTG CCGCCGAGGA ATTCAAGCGG 
AAGGCAAACG AATGTCAGCA CAAACTGATG AAGGTAGTCA GCCACCCTCC AAGAGGGGAC 
TCCGGGGGCA CGGCCCTGGA TGATCTTCAC AAGATGCAAG GCCACGCTGG CTTAACGAGT 
GCTAAGGACC AAGGGAAGCC TGAAGTGGGA GAATATTCCA AACTGGAGAA GATCAATGCT 
GAGCAGCAGC TGAAGATCCA GGAGCTCCAG GAGAAGCTGG AGAAGGCTGT AAAAGCCAGC 
ACAGAGGCCA CCGAGCTCCT GCAGAATATC CGCCAGGCAA AGGAGCGAGC AGAGCGGGAG 
CTAGAGAAGC TACACAACCG GGAAGACTCC TCCGAGGGCA TCAAAAAGAA GCTGGTGGAA 
GCCGAGGAAC GCCGCCACTC CCTGGAGAAC AAGGTAAAGA GACTAGAGAC CATGGAGCGT 
AGAGAGAACA GACTGAAGGA TGACATCCAG ACAAAGTCCG AACAGATCCA GCAGATGGCT 
GATAAAATTC TGGAGCTGGA GGAGAAACAT CGGGAGGCTC AGGTCTCAGC TCAACATCTA 
GAAGTACACT TGAAACAGAA AGAACAGCAC TACGAGGAAA AGATCAAGGT ATTGGACAAT 
CAGATAAAGA AGGACCTGGC CGACAAGGAG AGCCTGGAGA ACATGATGCA GAGACACGAG 
GAGGAGGCCC ACGAGAAGGG CAAGATCCTC AGCGAGCAGA AGGCGATGAT CAACGCGATG 
GATTCCAAGA TCCGATCCCT GGAGCAGAGG ATCGTGGAGC TGTCGGAAGC CAACAAGCTT 
GCGGCAAACA GCAGTCTCTT CACCCAGAGG AACATGAAGG CCCAGGAAGA GATGATCTCA 
GAACTCAGGC AGCAGAAATT TTACCTGGAG ACGCAGGCCG GAAAGCTGGA GGCCCAGAAC 
CGAAAGCTGG AAGAGCAACT GGAGAAGATC AGCCACCAAG ATCACAGTGA CAAGAGTCGG 
CTGCTGGAGC TGGAAACAAG GCTGAGGGAG GTCAGCCTGG AGCACGAGGA GCAGAAGCTG 
GAGCTGAAGC GGCAGCTCAC GGAGCTGCAG CTGTCCCTGC AGGAGCGCGA GTCCCAGCTG 
ACGGCCCTGC AGGCCGCCCG GGCAGCGCTG GAGAGCCAGC TCCGCCAGGC GAAGACAGAG 
CTGGAGGAGA CAACCGCGGA AGCGGAGGAG GAGATCCAGG CGCTCACGGC ACATCGAGAT 
GAGATCCAGC GCAAATTCGA TGCCCTTCGC AACAGCTGCA CGGTCATCAC CGACCTGGAG 
GAGCAGCTGA ACCAGCTCAC CGAGGACAAC GCCGAGCTCA ACAACCAAAA CTTCTACCTG 
TCCAAACAAC TCGATGAGGC TTCCGGGGCC AATGACGAGA TTGTGCAGCT GCGGAGTGAG 
GTGGACCATC TCCGCCGTGA GATCACGGAG CGGGAGATGC AGCTCACCAG CCAGAAGCAA 
GCACAACTCA GTGCTCCTGA TTTGCAGACG ATGGAGGCTC TGAAGACGAC ATGCACCATG 
CTGGAGGAGC AGGTCCTGGA CCTGGAGGCC CTGAACGACG AGCTGCTGGA GAAGGAGCGC 
CAGTGGGAGG CCTGGCGGAG CGTCCTCGGC GACGAGAAGT CCCAGTTCGA GTGTCGAGTT 
CGAGAGCTAC AGAGGATGCT GGACACCGAG AAGCAGAGCA GGGCTAGGGC CGATCAGCGG 
ATCACCGAGT CGCGCCAGGT GGTGGAGTTG GCGGTGAAGG AACACAAGGC CGAGATTCTT 
GCTCTGCAGC AGGCTCTCAA GGAGCAGAAG CTCAAAGCCG AGAGCCTGTC GGACAAGCTC 
AACGACCTGG AGAAGAAGCA CGCCATGCTG GAGATGAACG CCCGGAGCTT ACAGCAGAAA 
CTAGAGACAG AGCGGGAGCT CAAACAGAGG CTTCTGGAGG AGCAAGCCAA ATTGCAGCAG 
CAGATGGACC TGCAGAAGAA CCACATCTTC AGACTGACGC AAGGGCTGCA GGAGGCGCTG 
GACCGGGCGG ATCTGCTGAA GACAGAAAGG AGCGACCTGG AGTACCAGCT GGAAAACATT 
CAGGTTCTCT ACTCTCACGA GAAAGTGAAA ATGGAAGGCA CAATCTCTCA GCAAACCAAA 
CTCATTGATT TCCTGCAAGC CAAAATGGAC CAGCCTGCTA AAAAGAAAAA GGGTTTATTT 
AGTCGACGGA AAGAGGACCC TGCTTTGCCC ACACAGGTTC CTCTGCAGTA CAATGAGCTG 
AAGCTAGCCC TGGAAAAGGA GAAAGCCCGA TGCGCGGAGC TGGAGGAGGC CCTTCAGAAG 
ACCCGCATCG AACTCCGCTC TGCGCGGGAG GAAGCTGCCC ACCGCAAAGC CACGGACCAC 
CCGCACCCGT CTACGCCAGC CACTGCGAGG CAGCAGATCG CCATGTCTGC CATTGTGCGG 
TCGCCCGAGC ACCAGCCCAG TGCCATGAGC CTGCTTGCCC CACCATCCAG CCGCAGAAAG 
GAGTCGTCAA CTCCAGAGGA ATTCAGCCGG CGTCTGAAAG AGCGCATGCA CCACAACATC 
CCTCACCGGT TTAATGTGGG CCTGAACATG AGAGCCACCA AGTGCGCCGT GTGTCTGGAT 
ACTGTGCACT TTGGACGCCA GGCATCCAAA TGCCTCGAAT GTCAAGTGAT GTGTCACCCC 
AAATGCTCCA CCTGCTTGCC CGCTACCTGT GGCCTGCCAG CTGAATATGC CACACACTTC 
ACTGAGGCCT TCTGCCGGGA CAAAATGAAC TCCCCGGGGC TCCAGAGCAA GGAGCCTGGC 
AGCAGCTTGC ACCTGGAAGG GTGGATGAAA GTGCCCAGAA ATAACAAACG GGGACAGCAA 
GGCTGGGACA GGAAGTACAT TGTTCTGGAG GGGTCAAAAG TCCTCATCTA TGACAATGAA 
GCCAGAGAAG CTGGACAGAG GCCGGTGGAA GAATTTGAGC TGTGCCTTCC TGACGGGGAC 
GTATCTATTC ATGGTGCCGT TGGTGCTTCA GAACTTGCAA ATACCGCCAA AGCAGATGTC 
CCATACATCC TGAAGATGGA GTCTCACCCA CACACCACCT GCTGGCCTGG GAGAACCCTC 
TACTTGCTAG CACCCAGCTT CCCCGACAAG CAGCGCTGGG TCACCGCCTT AGAATCTGTC 
GTCGCAGGTG GGAGAGTTTC TAGGGAAAAG GCCGAAGCCG ATGCTGCACG TGACCATACT 
TCGAGTGAGC ATCAGCCAGT GTGGGTTGAG AAATTACTTG GAAACTCTCT GCTGAAACTG 
GAGGGCGATG ACCGGCTTGA CATGAACTGC ACCCTGCCCT TCAGTGACCA GGTAGTGCTG 
GTGGGCACGG AGGAAGGCCT CTACGCCCTG AATGTCTTGA AAAACTCCTT AACCCACATC 
CCAGGGATTG GCGCAGTCTT CCAAATTTAC ATCATCAAGG ACCTGGAGAA GCTGCTCATG 
ATAGCAGGGG AAGAGCGGGC TCTGTGCCTG GTGGACGTGA AGAAGGTGAA GCAGTCCCTG 
GCGCAGTCAC ACCTGCCTGC CCAGCCCGAC GTCTCCCCCA ACATATTCGA AGCCGTCAAA 
GGCTGCCACT TGTTCGCTGC TGGCAAGATC GAGAACAGCC TGTGCATCTG CGCCGCTATG 
CCAAGCAAAG TCGTCATCCT CCGCTACAAT GACAACCTCA GCAAGTACTG CATCCGCAAG 
GAGATCGAGA CCTCAGAGCC CTGCAGCTGT ATCCACTTCA CCAACTACAG CATCCTCATT 
GGGACCAACA AATTCTATGA GATCGACATG AAGCAGTACA CGCTTGACGA GTTCCTGGAC 
AAGAACGACC ATTCCTTGGC ACCTGCTGTG TTCGCCTCCT CGTCCAACAG CTTCCCTGTC 
TCCATTGTGC AGGCGAACAG CGCCGGGCAG CGAGAAGAAT ACCTGCTGTG CTTCCACGAA 
TTTGGGGTGT TCGTGGATTC TTACGGAAGA CGTAGCCGCA CAGATGATCT CAAGTGGAGT 
CGCTTACCTC TGGCCTTCGC CTACAGAGAA CCTTATCTGT TTGTGACTCA CTTCAACTCC 
CTGGAAGTCA TTGAGATCCA GGCCAGATCC TCACTGGGGA GCCCTGCCCG AGCATATCTG 
GAAATTCCAA ACCCTCGCTA CCTGGGCCCC GCGATTTCCT CCGGAGCGAT TTACCTGGCC 
TCCTCATACC AGGACAAGTT AAGGGTCATA TGCTGCAAAG GAAACCTCGT GAAGGAGTCA 
GGCACTGAGC AGCACCGGGT GCCCTCCACC TCCCGCAGCA GCCCCAACAA GCGAGGCCCA 
CCAACATACA ACGAGCACAT CACCAAACGC GTGGCCTCCA GCCCGGCGCC ACCGGAAGGC 
CCCAGCCACC CCCGAGAGCC AAGCACACCG CACCGCTACC GAGACAGAGA GGGTCGGACA 
GAGCTGCGCA GGGACAAGTC TCCAGGCCGC CCTCTGGAGC GGGAGAAGTC CCCAGGCCGA 
ATGCTCAGCA CTAGGAGAGA GCGGTCCCCA GGGAGACTGT TTGAAGACAG CAGCAGGGGC 
CGGCTGCCTG CAGGAGCAGT GAGGACCCCA CTGTCCCAGG TTAACAAGGT CTGGGACCAG 
TCTTCAGTAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cit ( XM_006530132.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006530195.1 Mus musculus citron (Cit), transcript variant X3, mRNA.

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CloneID	OMu35774
Clone ID Related Accession (Same CDS sequence)	XM_006530130.3 , XM_006530132.3 , XM_006530133.3 , XM_006530130.4 , XM_006530131.2 , XM_006530131.1 , XM_006530133.4 , XM_006530132.4
Accession Version	XM_006530132.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6372bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	citron Rho-interacting kinase isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000071.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006530132.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTGAAGT TCAAGTATGG TGTGCGGAAC CCGCCGGAGG CCAGTGCCTC CGAGCCCATT 
GCCAGTCGGG CCTCCAGGCT AAATCTCTTC TTCCAGGGGA AACCGCCCCT CATGACTCAA 
CAGCAGATGT CTGCTCTTTC CCGGGAAGGG ATGCTAGACG CCCTCTTCGC TCTCTTTGAA 
GAGTGCAGCC AACCCGCCCT GATGAAGATG AAGCACGTGA GCAGCTTTGT CCAGAAGTAT 
TCCGACACCA TAGCCGAGTT GCGGGAGCTG CAGCCGTCGG CGAGAGACTT CGAAGTTCGA 
AGCCTTGTGG GCTGTGGTCA CTTCGCTGAA GTGCAGGTGG TTAGAGAGAA GGCGACCGGG 
GACGTCTATG CCATGAAAAT CATGAAGAAG AAGGCTTTGC TGGCCCAGGA ACAGGTTTCA 
TTTTTCGAGG AGGAGAGGAA CATATTATCT CGGAGCACGA GTCCTTGGAT CCCCCAGTTA 
CAGTACGCCT TTCAGGACAA AAATAACCTT TACCTGGTCA TGGAATATCA GCCTGGAGGG 
GATTTGCTGT CGCTTCTGAA CAGATACGAG GACCAATTAG ATGAGAGCAT GATCCAGTTT 
TACCTTGCTG AGCTGATTTT GGCTGTCCAC AGCGTGCACC AGATGGGATA TGTGCATCGA 
GACATCAAGC CCGAGAACAT CCTCATCGAC CGGACGGGAC ACATCAAGCT GGTGGATTTT 
GGATCAGCCG CTAAGATGAA TTCAAATAAG GTGGATGCCA AACTCCCCAT TGGGACCCCG 
GATTACATGG CTCCGGAAGT GTTGACCGTG ATGAACGAGG ACCGAAGGGG CACATACGGC 
TTGGACTGTG ACTGGTGGTC TGTCGGAGTT GTTGCTTATG AGATGGTTTA TGGGAAGACC 
CCATTCACAG AGGGAACCTC CGCCCGGACC TTCAACAACA TCATGAACTT CCAGCGGTTT 
TTGAAGTTCC CAGATGACCC CAAAGTTAGC AGTGAGCTCC TTGATCTGCT TCAGAGTCTG 
CTGTGTGTCC AGAAAGAGAG ACTGAAGTTC GAGGGTCTCT GCTGCCACCC TTTCTTTGCC 
AGAACGGACT GGAACAACAT CCGTAACTCT CCTCCCCCCT TCGTCCCCAC CCTCAAGTCT 
GACGATGACA CCTCCAATTT TGATGAACCA GAGAAGAATT CGTGGGTTTC ATCCTCTGTG 
TGCCAGCTGA GCCCCTCGGG CTTCTCAGGC GAAGAGCTGC CGTTTGTGGG ATTTTCGTAC 
AGCAAGGCAC TGGGGTATCT TGGTAGATCT GAGTCTGTCG TGTCGAGTCT GGACTCCCCT 
GCCAAGGTTA GCTCCATGGA AAAGAAACTT CTCATCAAAA GCAAAGAGCT CCAAGACTCC 
CAGGACAAGT GTCACAAGAT GGAGCAGGAA ATGACCCGGT TACATCGCAG AGTGTCAGAG 
GTGGAGGCTG TGCTTAGTCA GAAGGAGGTG GAGCTGAAGG CCTCTGAGAC TCAGAGATCC 
CTCCTGGAGC AGGACCTTGC TACCTACATC ACAGAATGCA GTAGCTTAAA GCGAAGTTTG 
GAGCAAGCGC GGATGGAGGT GTCCCAGGAG GATGACAAAG CTCTGCAGCT TCTCCACGAC 
ATCCGAGAGC AGAGCCGGAA GCTCCAGGAG ATCAAGGAGC AGGAGTACCA GGCTCAGGTG 
GAGGAGATGA GGCTGATGAT GAATCAGCTG GAAGAAGACC TGGTGTCAGC CCGCAGACGC 
AGCGATCTCT ACGAGTCTGA GCTGAGGGAG TCTCGGCTTG CCGCCGAGGA ATTCAAGCGG 
AAGGCAAACG AATGTCAGCA CAAACTGATG AAGGTAGTCA GCCACCCTCC AAGAGGGGAC 
TCCGGGGGCA CGGCCCTGGA TGATCTTCAC AAGATGCAAG GCCACGCTGG CTTAACGAGT 
GCTAAGGACC AAGGGAAGCC TGAAGTGGGA GAATATTCCA AACTGGAGAA GATCAATGCT 
GAGCAGCAGC TGAAGATCCA GGAGCTCCAG GAGAAGCTGG AGAAGGCTGT AAAAGCCAGC 
ACAGAGGCCA CCGAGCTCCT GCAGAATATC CGCCAGGCAA AGGAGCGAGC AGAGCGGGAG 
CTAGAGAAGC TACACAACCG GGAAGACTCC TCCGAGGGCA TCAAAAAGAA GCTGGTGGAA 
GCCGAGGAAC GCCGCCACTC CCTGGAGAAC AAGGTAAAGA GACTAGAGAC CATGGAGCGT 
AGAGAGAACA GACTGAAGGA TGACATCCAG ACAAAGTCCG AACAGATCCA GCAGATGGCT 
GATAAAATTC TGGAGCTGGA GGAGAAACAT CGGGAGGCTC AGGTCTCAGC TCAACATCTA 
GAAGTACACT TGAAACAGAA AGAACAGCAC TACGAGGAAA AGATCAAGGT ATTGGACAAT 
CAGATAAAGA AGGACCTGGC CGACAAGGAG AGCCTGGAGA ACATGATGCA GAGACACGAG 
GAGGAGGCCC ACGAGAAGGG CAAGATCCTC AGCGAGCAGA AGGCGATGAT CAACGCGATG 
GATTCCAAGA TCCGATCCCT GGAGCAGAGG ATCGTGGAGC TGTCGGAAGC CAACAAGCTT 
GCGGCAAACA GCAGTCTCTT CACCCAGAGG AACATGAAGG CCCAGGAAGA GATGATCTCA 
GAACTCAGGC AGCAGAAATT TTACCTGGAG ACGCAGGCCG GAAAGCTGGA GGCCCAGAAC 
CGAAAGCTGG AAGAGCAACT GGAGAAGATC AGCCACCAAG ATCACAGTGA CAAGAGTCGG 
CTGCTGGAGC TGGAAACAAG GCTGAGGGAG GTCAGCCTGG AGCACGAGGA GCAGAAGCTG 
GAGCTGAAGC GGCAGCTCAC GGAGCTGCAG CTGTCCCTGC AGGAGCGCGA GTCCCAGCTG 
ACGGCCCTGC AGGCCGCCCG GGCAGCGCTG GAGAGCCAGC TCCGCCAGGC GAAGACAGAG 
CTGGAGGAGA CAACCGCGGA AGCGGAGGAG GAGATCCAGG CGCTCACGGC ACATCGAGAT 
GAGATCCAGC GCAAATTCGA TGCCCTTCGC AACAGCTGCA CGGTCATCAC CGACCTGGAG 
GAGCAGCTGA ACCAGCTCAC CGAGGACAAC GCCGAGCTCA ACAACCAAAA CTTCTACCTG 
TCCAAACAAC TCGATGAGGC TTCCGGGGCC AATGACGAGA TTGTGCAGCT GCGGAGTGAG 
GTGGACCATC TCCGCCGTGA GATCACGGAG CGGGAGATGC AGCTCACCAG CCAGAAGCAA 
GCACAACTCA GTGCTCCTGA TTTGCAGACG ATGGAGGCTC TGAAGACGAC ATGCACCATG 
CTGGAGGAGC AGGTCCTGGA CCTGGAGGCC CTGAACGACG AGCTGCTGGA GAAGGAGCGC 
CAGTGGGAGG CCTGGCGGAG CGTCCTCGGC GACGAGAAGT CCCAGTTCGA GTGTCGAGTT 
CGAGAGCTAC AGAGGATGCT GGACACCGAG AAGCAGAGCA GGGCTAGGGC CGATCAGCGG 
ATCACCGAGT CGCGCCAGGT GGTGGAGTTG GCGGTGAAGG AACACAAGGC CGAGATTCTT 
GCTCTGCAGC AGGCTCTCAA GGAGCAGAAG CTCAAAGCCG AGAGCCTGTC GGACAAGCTC 
AACGACCTGG AGAAGAAGCA CGCCATGCTG GAGATGAACG CCCGGAGCTT ACAGCAGAAA 
CTAGAGACAG AGCGGGAGCT CAAACAGAGG CTTCTGGAGG AGCAAGCCAA ATTGCAGCAG 
CAGATGGACC TGCAGAAGAA CCACATCTTC AGACTGACGC AAGGGCTGCA GGAGGCGCTG 
GACCGGGCGG ATCTGCTGAA GACAGAAAGG AGCGACCTGG AGTACCAGCT GGAAAACATT 
CAGGTTCTCT ACTCTCACGA GAAAGTGAAA ATGGAAGGCA CAATCTCTCA GCAAACCAAA 
CTCATTGATT TCCTGCAAGC CAAAATGGAC CAGCCTGCTA AAAAGAAAAA GGGTTTATTT 
AGTCGACGGA AAGAGGACCC TGCTTTGCCC ACACAGGTTC CTCTGCAGTA CAATGAGCTG 
AAGCTAGCCC TGGAAAAGGA GAAAGCCCGA TGCGCGGAGC TGGAGGAGGC CCTTCAGAAG 
ACCCGCATCG AACTCCGCTC TGCGCGGGAG GAAGCTGCCC ACCGCAAAGC CACGGACCAC 
CCGCACCCGT CTACGCCAGC CACTGCGAGG CAGCAGATCG CCATGTCTGC CATTGTGCGG 
TCGCCCGAGC ACCAGCCCAG TGCCATGAGC CTGCTTGCCC CACCATCCAG CCGCAGAAAG 
GAGTCGTCAA CTCCAGAGGA ATTCAGCCGG CGTCTGAAAG AGCGCATGCA CCACAACATC 
CCTCACCGGT TTAATGTGGG CCTGAACATG AGAGCCACCA AGTGCGCCGT GTGTCTGGAT 
ACTGTGCACT TTGGACGCCA GGCATCCAAA TGCCTCGAAT GTCAAGTGAT GTGTCACCCC 
AAATGCTCCA CCTGCTTGCC CGCTACCTGT GGCCTGCCAG CTGAATATGC CACACACTTC 
ACTGAGGCCT TCTGCCGGGA CAAAATGAAC TCCCCGGGGC TCCAGAGCAA GGAGCCTGGC 
AGCAGCTTGC ACCTGGAAGG GTGGATGAAA GTGCCCAGAA ATAACAAACG GGGACAGCAA 
GGCTGGGACA GGAAGTACAT TGTTCTGGAG GGGTCAAAAG TCCTCATCTA TGACAATGAA 
GCCAGAGAAG CTGGACAGAG GCCGGTGGAA GAATTTGAGC TGTGCCTTCC TGACGGGGAC 
GTATCTATTC ATGGTGCCGT TGGTGCTTCA GAACTTGCAA ATACCGCCAA AGCAGATGTC 
CCATACATCC TGAAGATGGA GTCTCACCCA CACACCACCT GCTGGCCTGG GAGAACCCTC 
TACTTGCTAG CACCCAGCTT CCCCGACAAG CAGCGCTGGG TCACCGCCTT AGAATCTGTC 
GTCGCAGGTG GGAGAGTTTC TAGGGAAAAG GCCGAAGCCG ATGCTGCACG TGACCATACT 
TCGAGTGAGC ATCAGCCAGT GTGGGTTGAG AAATTACTTG GAAACTCTCT GCTGAAACTG 
GAGGGCGATG ACCGGCTTGA CATGAACTGC ACCCTGCCCT TCAGTGACCA GGTAGTGCTG 
GTGGGCACGG AGGAAGGCCT CTACGCCCTG AATGTCTTGA AAAACTCCTT AACCCACATC 
CCAGGGATTG GCGCAGTCTT CCAAATTTAC ATCATCAAGG ACCTGGAGAA GCTGCTCATG 
ATAGCAGGGG AAGAGCGGGC TCTGTGCCTG GTGGACGTGA AGAAGGTGAA GCAGTCCCTG 
GCGCAGTCAC ACCTGCCTGC CCAGCCCGAC GTCTCCCCCA ACATATTCGA AGCCGTCAAA 
GGCTGCCACT TGTTCGCTGC TGGCAAGATC GAGAACAGCC TGTGCATCTG CGCCGCTATG 
CCAAGCAAAG TCGTCATCCT CCGCTACAAT GACAACCTCA GCAAGTACTG CATCCGCAAG 
GAGATCGAGA CCTCAGAGCC CTGCAGCTGT ATCCACTTCA CCAACTACAG CATCCTCATT 
GGGACCAACA AATTCTATGA GATCGACATG AAGCAGTACA CGCTTGACGA GTTCCTGGAC 
AAGAACGACC ATTCCTTGGC ACCTGCTGTG TTCGCCTCCT CGTCCAACAG CTTCCCTGTC 
TCCATTGTGC AGGCGAACAG CGCCGGGCAG CGAGAAGAAT ACCTGCTGTG CTTCCACGAA 
TTTGGGGTGT TCGTGGATTC TTACGGAAGA CGTAGCCGCA CAGATGATCT CAAGTGGAGT 
CGCTTACCTC TGGCCTTCGC CTACAGAGAA CCTTATCTGT TTGTGACTCA CTTCAACTCC 
CTGGAAGTCA TTGAGATCCA GGCCAGATCC TCACTGGGGA GCCCTGCCCG AGCATATCTG 
GAAATTCCAA ACCCTCGCTA CCTGGGCCCC GCGATTTCCT CCGGAGCGAT TTACCTGGCC 
TCCTCATACC AGGACAAGTT AAGGGTCATA TGCTGCAAAG GAAACCTCGT GAAGGAGTCA 
GGCACTGAGC AGCACCGGGT GCCCTCCACC TCCCGCAGCA GCCCCAACAA GCGAGGCCCA 
CCAACATACA ACGAGCACAT CACCAAACGC GTGGCCTCCA GCCCGGCGCC ACCGGAAGGC 
CCCAGCCACC CCCGAGAGCC AAGCACACCG CACCGCTACC GAGACAGAGA GGGTCGGACA 
GAGCTGCGCA GGGACAAGTC TCCAGGCCGC CCTCTGGAGC GGGAGAAGTC CCCAGGCCGA 
ATGCTCAGCA CTAGGAGAGA GCGGTCCCCA GGGAGACTGT TTGAAGACAG CAGCAGGGGC 
CGGCTGCCTG CAGGAGCAGT GAGGACCCCA CTGTCCCAGG TTAACAAGGT CTGGGACCAG 
TCTTCAGTAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006530195.1
CDS	125..6496
Translation	MLKFKYGVRNPPEASASEPIASRASRLNLFFQGKPPLMTQQQMSALSREGMLDALFALFEECSQPALMKMKHVSSFVQKYSDTIAELRELQPSARDFEVRSLVGCGHFAEVQVVREKATGDVYAMKIMKKKALLAQEQVSFFEEERNILSRSTSPWIPQLQYAFQDKNNLYLVMEYQPGGDLLSLLNRYEDQLDESMIQFYLAELILAVHSVHQMGYVHRDIKPENILIDRTGHIKLVDFGSAAKMNSNKVDAKLPIGTPDYMAPEVLTVMNEDRRGTYGLDCDWWSVGVVAYEMVYGKTPFTEGTSARTFNNIMNFQRFLKFPDDPKVSSELLDLLQSLLCVQKERLKFEGLCCHPFFARTDWNNIRNSPPPFVPTLKSDDDTSNFDEPEKNSWVSSSVCQLSPSGFSGEELPFVGFSYSKALGYLGRSESVVSSLDSPAKVSSMEKKLLIKSKELQDSQDKCHKMEQEMTRLHRRVSEVEAVLSQKEVELKASETQRSLLEQDLATYITECSSLKRSLEQARMEVSQEDDKALQLLHDIREQSRKLQEIKEQEYQAQVEEMRLMMNQLEEDLVSARRRSDLYESELRESRLAAEEFKRKANECQHKLMKVVSHPPRGDSGGTALDDLHKMQGHAGLTSAKDQGKPEVGEYSKLEKINAEQQLKIQELQEKLEKAVKASTEATELLQNIRQAKERAERELEKLHNREDSSEGIKKKLVEAEERRHSLENKVKRLETMERRENRLKDDIQTKSEQIQQMADKILELEEKHREAQVSAQHLEVHLKQKEQHYEEKIKVLDNQIKKDLADKESLENMMQRHEEEAHEKGKILSEQKAMINAMDSKIRSLEQRIVELSEANKLAANSSLFTQRNMKAQEEMISELRQQKFYLETQAGKLEAQNRKLEEQLEKISHQDHSDKSRLLELETRLREVSLEHEEQKLELKRQLTELQLSLQERESQLTALQAARAALESQLRQAKTELEETTAEAEEEIQALTAHRDEIQRKFDALRNSCTVITDLEEQLNQLTEDNAELNNQNFYLSKQLDEASGANDEIVQLRSEVDHLRREITEREMQLTSQKQAQLSAPDLQTMEALKTTCTMLEEQVLDLEALNDELLEKERQWEAWRSVLGDEKSQFECRVRELQRMLDTEKQSRARADQRITESRQVVELAVKEHKAEILALQQALKEQKLKAESLSDKLNDLEKKHAMLEMNARSLQQKLETERELKQRLLEEQAKLQQQMDLQKNHIFRLTQGLQEALDRADLLKTERSDLEYQLENIQVLYSHEKVKMEGTISQQTKLIDFLQAKMDQPAKKKKGLFSRRKEDPALPTQVPLQYNELKLALEKEKARCAELEEALQKTRIELRSAREEAAHRKATDHPHPSTPATARQQIAMSAIVRSPEHQPSAMSLLAPPSSRRKESSTPEEFSRRLKERMHHNIPHRFNVGLNMRATKCAVCLDTVHFGRQASKCLECQVMCHPKCSTCLPATCGLPAEYATHFTEAFCRDKMNSPGLQSKEPGSSLHLEGWMKVPRNNKRGQQGWDRKYIVLEGSKVLIYDNEAREAGQRPVEEFELCLPDGDVSIHGAVGASELANTAKADVPYILKMESHPHTTCWPGRTLYLLAPSFPDKQRWVTALESVVAGGRVSREKAEADAARDHTSSEHQPVWVEKLLGNSLLKLEGDDRLDMNCTLPFSDQVVLVGTEEGLYALNVLKNSLTHIPGIGAVFQIYIIKDLEKLLMIAGEERALCLVDVKKVKQSLAQSHLPAQPDVSPNIFEAVKGCHLFAAGKIENSLCICAAMPSKVVILRYNDNLSKYCIRKEIETSEPCSCIHFTNYSILIGTNKFYEIDMKQYTLDEFLDKNDHSLAPAVFASSSNSFPVSIVQANSAGQREEYLLCFHEFGVFVDSYGRRSRTDDLKWSRLPLAFAYREPYLFVTHFNSLEVIEIQARSSLGSPARAYLEIPNPRYLGPAISSGAIYLASSYQDKLRVICCKGNLVKESGTEQHRVPSTSRSSPNKRGPPTYNEHITKRVASSPAPPEGPSHPREPSTPHRYRDREGRTELRRDKSPGRPLEREKSPGRMLSTRRERSPGRLFEDSSRGRLPAGAVRTPLSQVNKVWDQSSV

Target ORF information:

RefSeq Version	XM_006530132.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus citron (Cit), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006530132.3

ORF Insert Sequence:

ATGTTGAAGT TCAAGTATGG TGTGCGGAAC CCGCCGGAGG CCAGTGCCTC CGAGCCCATT 
GCCAGTCGGG CCTCCAGGCT AAATCTCTTC TTCCAGGGGA AACCGCCCCT CATGACTCAA 
CAGCAGATGT CTGCTCTTTC CCGGGAAGGG ATGCTAGACG CCCTCTTCGC TCTCTTTGAA 
GAGTGCAGCC AACCCGCCCT GATGAAGATG AAGCACGTGA GCAGCTTTGT CCAGAAGTAT 
TCCGACACCA TAGCCGAGTT GCGGGAGCTG CAGCCGTCGG CGAGAGACTT CGAAGTTCGA 
AGCCTTGTGG GCTGTGGTCA CTTCGCTGAA GTGCAGGTGG TTAGAGAGAA GGCGACCGGG 
GACGTCTATG CCATGAAAAT CATGAAGAAG AAGGCTTTGC TGGCCCAGGA ACAGGTTTCA 
TTTTTCGAGG AGGAGAGGAA CATATTATCT CGGAGCACGA GTCCTTGGAT CCCCCAGTTA 
CAGTACGCCT TTCAGGACAA AAATAACCTT TACCTGGTCA TGGAATATCA GCCTGGAGGG 
GATTTGCTGT CGCTTCTGAA CAGATACGAG GACCAATTAG ATGAGAGCAT GATCCAGTTT 
TACCTTGCTG AGCTGATTTT GGCTGTCCAC AGCGTGCACC AGATGGGATA TGTGCATCGA 
GACATCAAGC CCGAGAACAT CCTCATCGAC CGGACGGGAC ACATCAAGCT GGTGGATTTT 
GGATCAGCCG CTAAGATGAA TTCAAATAAG GTGGATGCCA AACTCCCCAT TGGGACCCCG 
GATTACATGG CTCCGGAAGT GTTGACCGTG ATGAACGAGG ACCGAAGGGG CACATACGGC 
TTGGACTGTG ACTGGTGGTC TGTCGGAGTT GTTGCTTATG AGATGGTTTA TGGGAAGACC 
CCATTCACAG AGGGAACCTC CGCCCGGACC TTCAACAACA TCATGAACTT CCAGCGGTTT 
TTGAAGTTCC CAGATGACCC CAAAGTTAGC AGTGAGCTCC TTGATCTGCT TCAGAGTCTG 
CTGTGTGTCC AGAAAGAGAG ACTGAAGTTC GAGGGTCTCT GCTGCCACCC TTTCTTTGCC 
AGAACGGACT GGAACAACAT CCGTAACTCT CCTCCCCCCT TCGTCCCCAC CCTCAAGTCT 
GACGATGACA CCTCCAATTT TGATGAACCA GAGAAGAATT CGTGGGTTTC ATCCTCTGTG 
TGCCAGCTGA GCCCCTCGGG CTTCTCAGGC GAAGAGCTGC CGTTTGTGGG ATTTTCGTAC 
AGCAAGGCAC TGGGGTATCT TGGTAGATCT GAGTCTGTCG TGTCGAGTCT GGACTCCCCT 
GCCAAGGTTA GCTCCATGGA AAAGAAACTT CTCATCAAAA GCAAAGAGCT CCAAGACTCC 
CAGGACAAGT GTCACAAGAT GGAGCAGGAA ATGACCCGGT TACATCGCAG AGTGTCAGAG 
GTGGAGGCTG TGCTTAGTCA GAAGGAGGTG GAGCTGAAGG CCTCTGAGAC TCAGAGATCC 
CTCCTGGAGC AGGACCTTGC TACCTACATC ACAGAATGCA GTAGCTTAAA GCGAAGTTTG 
GAGCAAGCGC GGATGGAGGT GTCCCAGGAG GATGACAAAG CTCTGCAGCT TCTCCACGAC 
ATCCGAGAGC AGAGCCGGAA GCTCCAGGAG ATCAAGGAGC AGGAGTACCA GGCTCAGGTG 
GAGGAGATGA GGCTGATGAT GAATCAGCTG GAAGAAGACC TGGTGTCAGC CCGCAGACGC 
AGCGATCTCT ACGAGTCTGA GCTGAGGGAG TCTCGGCTTG CCGCCGAGGA ATTCAAGCGG 
AAGGCAAACG AATGTCAGCA CAAACTGATG AAGGTAGTCA GCCACCCTCC AAGAGGGGAC 
TCCGGGGGCA CGGCCCTGGA TGATCTTCAC AAGATGCAAG GCCACGCTGG CTTAACGAGT 
GCTAAGGACC AAGGGAAGCC TGAAGTGGGA GAATATTCCA AACTGGAGAA GATCAATGCT 
GAGCAGCAGC TGAAGATCCA GGAGCTCCAG GAGAAGCTGG AGAAGGCTGT AAAAGCCAGC 
ACAGAGGCCA CCGAGCTCCT GCAGAATATC CGCCAGGCAA AGGAGCGAGC AGAGCGGGAG 
CTAGAGAAGC TACACAACCG GGAAGACTCC TCCGAGGGCA TCAAAAAGAA GCTGGTGGAA 
GCCGAGGAAC GCCGCCACTC CCTGGAGAAC AAGGTAAAGA GACTAGAGAC CATGGAGCGT 
AGAGAGAACA GACTGAAGGA TGACATCCAG ACAAAGTCCG AACAGATCCA GCAGATGGCT 
GATAAAATTC TGGAGCTGGA GGAGAAACAT CGGGAGGCTC AGGTCTCAGC TCAACATCTA 
GAAGTACACT TGAAACAGAA AGAACAGCAC TACGAGGAAA AGATCAAGGT ATTGGACAAT 
CAGATAAAGA AGGACCTGGC CGACAAGGAG AGCCTGGAGA ACATGATGCA GAGACACGAG 
GAGGAGGCCC ACGAGAAGGG CAAGATCCTC AGCGAGCAGA AGGCGATGAT CAACGCGATG 
GATTCCAAGA TCCGATCCCT GGAGCAGAGG ATCGTGGAGC TGTCGGAAGC CAACAAGCTT 
GCGGCAAACA GCAGTCTCTT CACCCAGAGG AACATGAAGG CCCAGGAAGA GATGATCTCA 
GAACTCAGGC AGCAGAAATT TTACCTGGAG ACGCAGGCCG GAAAGCTGGA GGCCCAGAAC 
CGAAAGCTGG AAGAGCAACT GGAGAAGATC AGCCACCAAG ATCACAGTGA CAAGAGTCGG 
CTGCTGGAGC TGGAAACAAG GCTGAGGGAG GTCAGCCTGG AGCACGAGGA GCAGAAGCTG 
GAGCTGAAGC GGCAGCTCAC GGAGCTGCAG CTGTCCCTGC AGGAGCGCGA GTCCCAGCTG 
ACGGCCCTGC AGGCCGCCCG GGCAGCGCTG GAGAGCCAGC TCCGCCAGGC GAAGACAGAG 
CTGGAGGAGA CAACCGCGGA AGCGGAGGAG GAGATCCAGG CGCTCACGGC ACATCGAGAT 
GAGATCCAGC GCAAATTCGA TGCCCTTCGC AACAGCTGCA CGGTCATCAC CGACCTGGAG 
GAGCAGCTGA ACCAGCTCAC CGAGGACAAC GCCGAGCTCA ACAACCAAAA CTTCTACCTG 
TCCAAACAAC TCGATGAGGC TTCCGGGGCC AATGACGAGA TTGTGCAGCT GCGGAGTGAG 
GTGGACCATC TCCGCCGTGA GATCACGGAG CGGGAGATGC AGCTCACCAG CCAGAAGCAA 
GCACAACTCA GTGCTCCTGA TTTGCAGACG ATGGAGGCTC TGAAGACGAC ATGCACCATG 
CTGGAGGAGC AGGTCCTGGA CCTGGAGGCC CTGAACGACG AGCTGCTGGA GAAGGAGCGC 
CAGTGGGAGG CCTGGCGGAG CGTCCTCGGC GACGAGAAGT CCCAGTTCGA GTGTCGAGTT 
CGAGAGCTAC AGAGGATGCT GGACACCGAG AAGCAGAGCA GGGCTAGGGC CGATCAGCGG 
ATCACCGAGT CGCGCCAGGT GGTGGAGTTG GCGGTGAAGG AACACAAGGC CGAGATTCTT 
GCTCTGCAGC AGGCTCTCAA GGAGCAGAAG CTCAAAGCCG AGAGCCTGTC GGACAAGCTC 
AACGACCTGG AGAAGAAGCA CGCCATGCTG GAGATGAACG CCCGGAGCTT ACAGCAGAAA 
CTAGAGACAG AGCGGGAGCT CAAACAGAGG CTTCTGGAGG AGCAAGCCAA ATTGCAGCAG 
CAGATGGACC TGCAGAAGAA CCACATCTTC AGACTGACGC AAGGGCTGCA GGAGGCGCTG 
GACCGGGCGG ATCTGCTGAA GACAGAAAGG AGCGACCTGG AGTACCAGCT GGAAAACATT 
CAGGTTCTCT ACTCTCACGA GAAAGTGAAA ATGGAAGGCA CAATCTCTCA GCAAACCAAA 
CTCATTGATT TCCTGCAAGC CAAAATGGAC CAGCCTGCTA AAAAGAAAAA GGGTTTATTT 
AGTCGACGGA AAGAGGACCC TGCTTTGCCC ACACAGGTTC CTCTGCAGTA CAATGAGCTG 
AAGCTAGCCC TGGAAAAGGA GAAAGCCCGA TGCGCGGAGC TGGAGGAGGC CCTTCAGAAG 
ACCCGCATCG AACTCCGCTC TGCGCGGGAG GAAGCTGCCC ACCGCAAAGC CACGGACCAC 
CCGCACCCGT CTACGCCAGC CACTGCGAGG CAGCAGATCG CCATGTCTGC CATTGTGCGG 
TCGCCCGAGC ACCAGCCCAG TGCCATGAGC CTGCTTGCCC CACCATCCAG CCGCAGAAAG 
GAGTCGTCAA CTCCAGAGGA ATTCAGCCGG CGTCTGAAAG AGCGCATGCA CCACAACATC 
CCTCACCGGT TTAATGTGGG CCTGAACATG AGAGCCACCA AGTGCGCCGT GTGTCTGGAT 
ACTGTGCACT TTGGACGCCA GGCATCCAAA TGCCTCGAAT GTCAAGTGAT GTGTCACCCC 
AAATGCTCCA CCTGCTTGCC CGCTACCTGT GGCCTGCCAG CTGAATATGC CACACACTTC 
ACTGAGGCCT TCTGCCGGGA CAAAATGAAC TCCCCGGGGC TCCAGAGCAA GGAGCCTGGC 
AGCAGCTTGC ACCTGGAAGG GTGGATGAAA GTGCCCAGAA ATAACAAACG GGGACAGCAA 
GGCTGGGACA GGAAGTACAT TGTTCTGGAG GGGTCAAAAG TCCTCATCTA TGACAATGAA 
GCCAGAGAAG CTGGACAGAG GCCGGTGGAA GAATTTGAGC TGTGCCTTCC TGACGGGGAC 
GTATCTATTC ATGGTGCCGT TGGTGCTTCA GAACTTGCAA ATACCGCCAA AGCAGATGTC 
CCATACATCC TGAAGATGGA GTCTCACCCA CACACCACCT GCTGGCCTGG GAGAACCCTC 
TACTTGCTAG CACCCAGCTT CCCCGACAAG CAGCGCTGGG TCACCGCCTT AGAATCTGTC 
GTCGCAGGTG GGAGAGTTTC TAGGGAAAAG GCCGAAGCCG ATGCTGCACG TGACCATACT 
TCGAGTGAGC ATCAGCCAGT GTGGGTTGAG AAATTACTTG GAAACTCTCT GCTGAAACTG 
GAGGGCGATG ACCGGCTTGA CATGAACTGC ACCCTGCCCT TCAGTGACCA GGTAGTGCTG 
GTGGGCACGG AGGAAGGCCT CTACGCCCTG AATGTCTTGA AAAACTCCTT AACCCACATC 
CCAGGGATTG GCGCAGTCTT CCAAATTTAC ATCATCAAGG ACCTGGAGAA GCTGCTCATG 
ATAGCAGGGG AAGAGCGGGC TCTGTGCCTG GTGGACGTGA AGAAGGTGAA GCAGTCCCTG 
GCGCAGTCAC ACCTGCCTGC CCAGCCCGAC GTCTCCCCCA ACATATTCGA AGCCGTCAAA 
GGCTGCCACT TGTTCGCTGC TGGCAAGATC GAGAACAGCC TGTGCATCTG CGCCGCTATG 
CCAAGCAAAG TCGTCATCCT CCGCTACAAT GACAACCTCA GCAAGTACTG CATCCGCAAG 
GAGATCGAGA CCTCAGAGCC CTGCAGCTGT ATCCACTTCA CCAACTACAG CATCCTCATT 
GGGACCAACA AATTCTATGA GATCGACATG AAGCAGTACA CGCTTGACGA GTTCCTGGAC 
AAGAACGACC ATTCCTTGGC ACCTGCTGTG TTCGCCTCCT CGTCCAACAG CTTCCCTGTC 
TCCATTGTGC AGGCGAACAG CGCCGGGCAG CGAGAAGAAT ACCTGCTGTG CTTCCACGAA 
TTTGGGGTGT TCGTGGATTC TTACGGAAGA CGTAGCCGCA CAGATGATCT CAAGTGGAGT 
CGCTTACCTC TGGCCTTCGC CTACAGAGAA CCTTATCTGT TTGTGACTCA CTTCAACTCC 
CTGGAAGTCA TTGAGATCCA GGCCAGATCC TCACTGGGGA GCCCTGCCCG AGCATATCTG 
GAAATTCCAA ACCCTCGCTA CCTGGGCCCC GCGATTTCCT CCGGAGCGAT TTACCTGGCC 
TCCTCATACC AGGACAAGTT AAGGGTCATA TGCTGCAAAG GAAACCTCGT GAAGGAGTCA 
GGCACTGAGC AGCACCGGGT GCCCTCCACC TCCCGCAGCA GCCCCAACAA GCGAGGCCCA 
CCAACATACA ACGAGCACAT CACCAAACGC GTGGCCTCCA GCCCGGCGCC ACCGGAAGGC 
CCCAGCCACC CCCGAGAGCC AAGCACACCG CACCGCTACC GAGACAGAGA GGGTCGGACA 
GAGCTGCGCA GGGACAAGTC TCCAGGCCGC CCTCTGGAGC GGGAGAAGTC CCCAGGCCGA 
ATGCTCAGCA CTAGGAGAGA GCGGTCCCCA GGGAGACTGT TTGAAGACAG CAGCAGGGGC 
CGGCTGCCTG CAGGAGCAGT GAGGACCCCA CTGTCCCAGG TTAACAAGGT CTGGGACCAG 
TCTTCAGTAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cit ( XM_006530133.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006530196.1 Mus musculus citron (Cit), transcript variant X4, mRNA.

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CloneID	OMu35774
Clone ID Related Accession (Same CDS sequence)	XM_006530130.3 , XM_006530132.3 , XM_006530133.3 , XM_006530130.4 , XM_006530131.2 , XM_006530131.1 , XM_006530133.4 , XM_006530132.4
Accession Version	XM_006530133.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6372bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	citron Rho-interacting kinase isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000071.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006530133.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTGAAGT TCAAGTATGG TGTGCGGAAC CCGCCGGAGG CCAGTGCCTC CGAGCCCATT 
GCCAGTCGGG CCTCCAGGCT AAATCTCTTC TTCCAGGGGA AACCGCCCCT CATGACTCAA 
CAGCAGATGT CTGCTCTTTC CCGGGAAGGG ATGCTAGACG CCCTCTTCGC TCTCTTTGAA 
GAGTGCAGCC AACCCGCCCT GATGAAGATG AAGCACGTGA GCAGCTTTGT CCAGAAGTAT 
TCCGACACCA TAGCCGAGTT GCGGGAGCTG CAGCCGTCGG CGAGAGACTT CGAAGTTCGA 
AGCCTTGTGG GCTGTGGTCA CTTCGCTGAA GTGCAGGTGG TTAGAGAGAA GGCGACCGGG 
GACGTCTATG CCATGAAAAT CATGAAGAAG AAGGCTTTGC TGGCCCAGGA ACAGGTTTCA 
TTTTTCGAGG AGGAGAGGAA CATATTATCT CGGAGCACGA GTCCTTGGAT CCCCCAGTTA 
CAGTACGCCT TTCAGGACAA AAATAACCTT TACCTGGTCA TGGAATATCA GCCTGGAGGG 
GATTTGCTGT CGCTTCTGAA CAGATACGAG GACCAATTAG ATGAGAGCAT GATCCAGTTT 
TACCTTGCTG AGCTGATTTT GGCTGTCCAC AGCGTGCACC AGATGGGATA TGTGCATCGA 
GACATCAAGC CCGAGAACAT CCTCATCGAC CGGACGGGAC ACATCAAGCT GGTGGATTTT 
GGATCAGCCG CTAAGATGAA TTCAAATAAG GTGGATGCCA AACTCCCCAT TGGGACCCCG 
GATTACATGG CTCCGGAAGT GTTGACCGTG ATGAACGAGG ACCGAAGGGG CACATACGGC 
TTGGACTGTG ACTGGTGGTC TGTCGGAGTT GTTGCTTATG AGATGGTTTA TGGGAAGACC 
CCATTCACAG AGGGAACCTC CGCCCGGACC TTCAACAACA TCATGAACTT CCAGCGGTTT 
TTGAAGTTCC CAGATGACCC CAAAGTTAGC AGTGAGCTCC TTGATCTGCT TCAGAGTCTG 
CTGTGTGTCC AGAAAGAGAG ACTGAAGTTC GAGGGTCTCT GCTGCCACCC TTTCTTTGCC 
AGAACGGACT GGAACAACAT CCGTAACTCT CCTCCCCCCT TCGTCCCCAC CCTCAAGTCT 
GACGATGACA CCTCCAATTT TGATGAACCA GAGAAGAATT CGTGGGTTTC ATCCTCTGTG 
TGCCAGCTGA GCCCCTCGGG CTTCTCAGGC GAAGAGCTGC CGTTTGTGGG ATTTTCGTAC 
AGCAAGGCAC TGGGGTATCT TGGTAGATCT GAGTCTGTCG TGTCGAGTCT GGACTCCCCT 
GCCAAGGTTA GCTCCATGGA AAAGAAACTT CTCATCAAAA GCAAAGAGCT CCAAGACTCC 
CAGGACAAGT GTCACAAGAT GGAGCAGGAA ATGACCCGGT TACATCGCAG AGTGTCAGAG 
GTGGAGGCTG TGCTTAGTCA GAAGGAGGTG GAGCTGAAGG CCTCTGAGAC TCAGAGATCC 
CTCCTGGAGC AGGACCTTGC TACCTACATC ACAGAATGCA GTAGCTTAAA GCGAAGTTTG 
GAGCAAGCGC GGATGGAGGT GTCCCAGGAG GATGACAAAG CTCTGCAGCT TCTCCACGAC 
ATCCGAGAGC AGAGCCGGAA GCTCCAGGAG ATCAAGGAGC AGGAGTACCA GGCTCAGGTG 
GAGGAGATGA GGCTGATGAT GAATCAGCTG GAAGAAGACC TGGTGTCAGC CCGCAGACGC 
AGCGATCTCT ACGAGTCTGA GCTGAGGGAG TCTCGGCTTG CCGCCGAGGA ATTCAAGCGG 
AAGGCAAACG AATGTCAGCA CAAACTGATG AAGGTAGTCA GCCACCCTCC AAGAGGGGAC 
TCCGGGGGCA CGGCCCTGGA TGATCTTCAC AAGATGCAAG GCCACGCTGG CTTAACGAGT 
GCTAAGGACC AAGGGAAGCC TGAAGTGGGA GAATATTCCA AACTGGAGAA GATCAATGCT 
GAGCAGCAGC TGAAGATCCA GGAGCTCCAG GAGAAGCTGG AGAAGGCTGT AAAAGCCAGC 
ACAGAGGCCA CCGAGCTCCT GCAGAATATC CGCCAGGCAA AGGAGCGAGC AGAGCGGGAG 
CTAGAGAAGC TACACAACCG GGAAGACTCC TCCGAGGGCA TCAAAAAGAA GCTGGTGGAA 
GCCGAGGAAC GCCGCCACTC CCTGGAGAAC AAGGTAAAGA GACTAGAGAC CATGGAGCGT 
AGAGAGAACA GACTGAAGGA TGACATCCAG ACAAAGTCCG AACAGATCCA GCAGATGGCT 
GATAAAATTC TGGAGCTGGA GGAGAAACAT CGGGAGGCTC AGGTCTCAGC TCAACATCTA 
GAAGTACACT TGAAACAGAA AGAACAGCAC TACGAGGAAA AGATCAAGGT ATTGGACAAT 
CAGATAAAGA AGGACCTGGC CGACAAGGAG AGCCTGGAGA ACATGATGCA GAGACACGAG 
GAGGAGGCCC ACGAGAAGGG CAAGATCCTC AGCGAGCAGA AGGCGATGAT CAACGCGATG 
GATTCCAAGA TCCGATCCCT GGAGCAGAGG ATCGTGGAGC TGTCGGAAGC CAACAAGCTT 
GCGGCAAACA GCAGTCTCTT CACCCAGAGG AACATGAAGG CCCAGGAAGA GATGATCTCA 
GAACTCAGGC AGCAGAAATT TTACCTGGAG ACGCAGGCCG GAAAGCTGGA GGCCCAGAAC 
CGAAAGCTGG AAGAGCAACT GGAGAAGATC AGCCACCAAG ATCACAGTGA CAAGAGTCGG 
CTGCTGGAGC TGGAAACAAG GCTGAGGGAG GTCAGCCTGG AGCACGAGGA GCAGAAGCTG 
GAGCTGAAGC GGCAGCTCAC GGAGCTGCAG CTGTCCCTGC AGGAGCGCGA GTCCCAGCTG 
ACGGCCCTGC AGGCCGCCCG GGCAGCGCTG GAGAGCCAGC TCCGCCAGGC GAAGACAGAG 
CTGGAGGAGA CAACCGCGGA AGCGGAGGAG GAGATCCAGG CGCTCACGGC ACATCGAGAT 
GAGATCCAGC GCAAATTCGA TGCCCTTCGC AACAGCTGCA CGGTCATCAC CGACCTGGAG 
GAGCAGCTGA ACCAGCTCAC CGAGGACAAC GCCGAGCTCA ACAACCAAAA CTTCTACCTG 
TCCAAACAAC TCGATGAGGC TTCCGGGGCC AATGACGAGA TTGTGCAGCT GCGGAGTGAG 
GTGGACCATC TCCGCCGTGA GATCACGGAG CGGGAGATGC AGCTCACCAG CCAGAAGCAA 
GCACAACTCA GTGCTCCTGA TTTGCAGACG ATGGAGGCTC TGAAGACGAC ATGCACCATG 
CTGGAGGAGC AGGTCCTGGA CCTGGAGGCC CTGAACGACG AGCTGCTGGA GAAGGAGCGC 
CAGTGGGAGG CCTGGCGGAG CGTCCTCGGC GACGAGAAGT CCCAGTTCGA GTGTCGAGTT 
CGAGAGCTAC AGAGGATGCT GGACACCGAG AAGCAGAGCA GGGCTAGGGC CGATCAGCGG 
ATCACCGAGT CGCGCCAGGT GGTGGAGTTG GCGGTGAAGG AACACAAGGC CGAGATTCTT 
GCTCTGCAGC AGGCTCTCAA GGAGCAGAAG CTCAAAGCCG AGAGCCTGTC GGACAAGCTC 
AACGACCTGG AGAAGAAGCA CGCCATGCTG GAGATGAACG CCCGGAGCTT ACAGCAGAAA 
CTAGAGACAG AGCGGGAGCT CAAACAGAGG CTTCTGGAGG AGCAAGCCAA ATTGCAGCAG 
CAGATGGACC TGCAGAAGAA CCACATCTTC AGACTGACGC AAGGGCTGCA GGAGGCGCTG 
GACCGGGCGG ATCTGCTGAA GACAGAAAGG AGCGACCTGG AGTACCAGCT GGAAAACATT 
CAGGTTCTCT ACTCTCACGA GAAAGTGAAA ATGGAAGGCA CAATCTCTCA GCAAACCAAA 
CTCATTGATT TCCTGCAAGC CAAAATGGAC CAGCCTGCTA AAAAGAAAAA GGGTTTATTT 
AGTCGACGGA AAGAGGACCC TGCTTTGCCC ACACAGGTTC CTCTGCAGTA CAATGAGCTG 
AAGCTAGCCC TGGAAAAGGA GAAAGCCCGA TGCGCGGAGC TGGAGGAGGC CCTTCAGAAG 
ACCCGCATCG AACTCCGCTC TGCGCGGGAG GAAGCTGCCC ACCGCAAAGC CACGGACCAC 
CCGCACCCGT CTACGCCAGC CACTGCGAGG CAGCAGATCG CCATGTCTGC CATTGTGCGG 
TCGCCCGAGC ACCAGCCCAG TGCCATGAGC CTGCTTGCCC CACCATCCAG CCGCAGAAAG 
GAGTCGTCAA CTCCAGAGGA ATTCAGCCGG CGTCTGAAAG AGCGCATGCA CCACAACATC 
CCTCACCGGT TTAATGTGGG CCTGAACATG AGAGCCACCA AGTGCGCCGT GTGTCTGGAT 
ACTGTGCACT TTGGACGCCA GGCATCCAAA TGCCTCGAAT GTCAAGTGAT GTGTCACCCC 
AAATGCTCCA CCTGCTTGCC CGCTACCTGT GGCCTGCCAG CTGAATATGC CACACACTTC 
ACTGAGGCCT TCTGCCGGGA CAAAATGAAC TCCCCGGGGC TCCAGAGCAA GGAGCCTGGC 
AGCAGCTTGC ACCTGGAAGG GTGGATGAAA GTGCCCAGAA ATAACAAACG GGGACAGCAA 
GGCTGGGACA GGAAGTACAT TGTTCTGGAG GGGTCAAAAG TCCTCATCTA TGACAATGAA 
GCCAGAGAAG CTGGACAGAG GCCGGTGGAA GAATTTGAGC TGTGCCTTCC TGACGGGGAC 
GTATCTATTC ATGGTGCCGT TGGTGCTTCA GAACTTGCAA ATACCGCCAA AGCAGATGTC 
CCATACATCC TGAAGATGGA GTCTCACCCA CACACCACCT GCTGGCCTGG GAGAACCCTC 
TACTTGCTAG CACCCAGCTT CCCCGACAAG CAGCGCTGGG TCACCGCCTT AGAATCTGTC 
GTCGCAGGTG GGAGAGTTTC TAGGGAAAAG GCCGAAGCCG ATGCTGCACG TGACCATACT 
TCGAGTGAGC ATCAGCCAGT GTGGGTTGAG AAATTACTTG GAAACTCTCT GCTGAAACTG 
GAGGGCGATG ACCGGCTTGA CATGAACTGC ACCCTGCCCT TCAGTGACCA GGTAGTGCTG 
GTGGGCACGG AGGAAGGCCT CTACGCCCTG AATGTCTTGA AAAACTCCTT AACCCACATC 
CCAGGGATTG GCGCAGTCTT CCAAATTTAC ATCATCAAGG ACCTGGAGAA GCTGCTCATG 
ATAGCAGGGG AAGAGCGGGC TCTGTGCCTG GTGGACGTGA AGAAGGTGAA GCAGTCCCTG 
GCGCAGTCAC ACCTGCCTGC CCAGCCCGAC GTCTCCCCCA ACATATTCGA AGCCGTCAAA 
GGCTGCCACT TGTTCGCTGC TGGCAAGATC GAGAACAGCC TGTGCATCTG CGCCGCTATG 
CCAAGCAAAG TCGTCATCCT CCGCTACAAT GACAACCTCA GCAAGTACTG CATCCGCAAG 
GAGATCGAGA CCTCAGAGCC CTGCAGCTGT ATCCACTTCA CCAACTACAG CATCCTCATT 
GGGACCAACA AATTCTATGA GATCGACATG AAGCAGTACA CGCTTGACGA GTTCCTGGAC 
AAGAACGACC ATTCCTTGGC ACCTGCTGTG TTCGCCTCCT CGTCCAACAG CTTCCCTGTC 
TCCATTGTGC AGGCGAACAG CGCCGGGCAG CGAGAAGAAT ACCTGCTGTG CTTCCACGAA 
TTTGGGGTGT TCGTGGATTC TTACGGAAGA CGTAGCCGCA CAGATGATCT CAAGTGGAGT 
CGCTTACCTC TGGCCTTCGC CTACAGAGAA CCTTATCTGT TTGTGACTCA CTTCAACTCC 
CTGGAAGTCA TTGAGATCCA GGCCAGATCC TCACTGGGGA GCCCTGCCCG AGCATATCTG 
GAAATTCCAA ACCCTCGCTA CCTGGGCCCC GCGATTTCCT CCGGAGCGAT TTACCTGGCC 
TCCTCATACC AGGACAAGTT AAGGGTCATA TGCTGCAAAG GAAACCTCGT GAAGGAGTCA 
GGCACTGAGC AGCACCGGGT GCCCTCCACC TCCCGCAGCA GCCCCAACAA GCGAGGCCCA 
CCAACATACA ACGAGCACAT CACCAAACGC GTGGCCTCCA GCCCGGCGCC ACCGGAAGGC 
CCCAGCCACC CCCGAGAGCC AAGCACACCG CACCGCTACC GAGACAGAGA GGGTCGGACA 
GAGCTGCGCA GGGACAAGTC TCCAGGCCGC CCTCTGGAGC GGGAGAAGTC CCCAGGCCGA 
ATGCTCAGCA CTAGGAGAGA GCGGTCCCCA GGGAGACTGT TTGAAGACAG CAGCAGGGGC 
CGGCTGCCTG CAGGAGCAGT GAGGACCCCA CTGTCCCAGG TTAACAAGGT CTGGGACCAG 
TCTTCAGTAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006530196.1
CDS	115..6486
Translation	MLKFKYGVRNPPEASASEPIASRASRLNLFFQGKPPLMTQQQMSALSREGMLDALFALFEECSQPALMKMKHVSSFVQKYSDTIAELRELQPSARDFEVRSLVGCGHFAEVQVVREKATGDVYAMKIMKKKALLAQEQVSFFEEERNILSRSTSPWIPQLQYAFQDKNNLYLVMEYQPGGDLLSLLNRYEDQLDESMIQFYLAELILAVHSVHQMGYVHRDIKPENILIDRTGHIKLVDFGSAAKMNSNKVDAKLPIGTPDYMAPEVLTVMNEDRRGTYGLDCDWWSVGVVAYEMVYGKTPFTEGTSARTFNNIMNFQRFLKFPDDPKVSSELLDLLQSLLCVQKERLKFEGLCCHPFFARTDWNNIRNSPPPFVPTLKSDDDTSNFDEPEKNSWVSSSVCQLSPSGFSGEELPFVGFSYSKALGYLGRSESVVSSLDSPAKVSSMEKKLLIKSKELQDSQDKCHKMEQEMTRLHRRVSEVEAVLSQKEVELKASETQRSLLEQDLATYITECSSLKRSLEQARMEVSQEDDKALQLLHDIREQSRKLQEIKEQEYQAQVEEMRLMMNQLEEDLVSARRRSDLYESELRESRLAAEEFKRKANECQHKLMKVVSHPPRGDSGGTALDDLHKMQGHAGLTSAKDQGKPEVGEYSKLEKINAEQQLKIQELQEKLEKAVKASTEATELLQNIRQAKERAERELEKLHNREDSSEGIKKKLVEAEERRHSLENKVKRLETMERRENRLKDDIQTKSEQIQQMADKILELEEKHREAQVSAQHLEVHLKQKEQHYEEKIKVLDNQIKKDLADKESLENMMQRHEEEAHEKGKILSEQKAMINAMDSKIRSLEQRIVELSEANKLAANSSLFTQRNMKAQEEMISELRQQKFYLETQAGKLEAQNRKLEEQLEKISHQDHSDKSRLLELETRLREVSLEHEEQKLELKRQLTELQLSLQERESQLTALQAARAALESQLRQAKTELEETTAEAEEEIQALTAHRDEIQRKFDALRNSCTVITDLEEQLNQLTEDNAELNNQNFYLSKQLDEASGANDEIVQLRSEVDHLRREITEREMQLTSQKQAQLSAPDLQTMEALKTTCTMLEEQVLDLEALNDELLEKERQWEAWRSVLGDEKSQFECRVRELQRMLDTEKQSRARADQRITESRQVVELAVKEHKAEILALQQALKEQKLKAESLSDKLNDLEKKHAMLEMNARSLQQKLETERELKQRLLEEQAKLQQQMDLQKNHIFRLTQGLQEALDRADLLKTERSDLEYQLENIQVLYSHEKVKMEGTISQQTKLIDFLQAKMDQPAKKKKGLFSRRKEDPALPTQVPLQYNELKLALEKEKARCAELEEALQKTRIELRSAREEAAHRKATDHPHPSTPATARQQIAMSAIVRSPEHQPSAMSLLAPPSSRRKESSTPEEFSRRLKERMHHNIPHRFNVGLNMRATKCAVCLDTVHFGRQASKCLECQVMCHPKCSTCLPATCGLPAEYATHFTEAFCRDKMNSPGLQSKEPGSSLHLEGWMKVPRNNKRGQQGWDRKYIVLEGSKVLIYDNEAREAGQRPVEEFELCLPDGDVSIHGAVGASELANTAKADVPYILKMESHPHTTCWPGRTLYLLAPSFPDKQRWVTALESVVAGGRVSREKAEADAARDHTSSEHQPVWVEKLLGNSLLKLEGDDRLDMNCTLPFSDQVVLVGTEEGLYALNVLKNSLTHIPGIGAVFQIYIIKDLEKLLMIAGEERALCLVDVKKVKQSLAQSHLPAQPDVSPNIFEAVKGCHLFAAGKIENSLCICAAMPSKVVILRYNDNLSKYCIRKEIETSEPCSCIHFTNYSILIGTNKFYEIDMKQYTLDEFLDKNDHSLAPAVFASSSNSFPVSIVQANSAGQREEYLLCFHEFGVFVDSYGRRSRTDDLKWSRLPLAFAYREPYLFVTHFNSLEVIEIQARSSLGSPARAYLEIPNPRYLGPAISSGAIYLASSYQDKLRVICCKGNLVKESGTEQHRVPSTSRSSPNKRGPPTYNEHITKRVASSPAPPEGPSHPREPSTPHRYRDREGRTELRRDKSPGRPLEREKSPGRMLSTRRERSPGRLFEDSSRGRLPAGAVRTPLSQVNKVWDQSSV

Target ORF information:

RefSeq Version	XM_006530133.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus citron (Cit), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006530133.3

ORF Insert Sequence:

ATGTTGAAGT TCAAGTATGG TGTGCGGAAC CCGCCGGAGG CCAGTGCCTC CGAGCCCATT 
GCCAGTCGGG CCTCCAGGCT AAATCTCTTC TTCCAGGGGA AACCGCCCCT CATGACTCAA 
CAGCAGATGT CTGCTCTTTC CCGGGAAGGG ATGCTAGACG CCCTCTTCGC TCTCTTTGAA 
GAGTGCAGCC AACCCGCCCT GATGAAGATG AAGCACGTGA GCAGCTTTGT CCAGAAGTAT 
TCCGACACCA TAGCCGAGTT GCGGGAGCTG CAGCCGTCGG CGAGAGACTT CGAAGTTCGA 
AGCCTTGTGG GCTGTGGTCA CTTCGCTGAA GTGCAGGTGG TTAGAGAGAA GGCGACCGGG 
GACGTCTATG CCATGAAAAT CATGAAGAAG AAGGCTTTGC TGGCCCAGGA ACAGGTTTCA 
TTTTTCGAGG AGGAGAGGAA CATATTATCT CGGAGCACGA GTCCTTGGAT CCCCCAGTTA 
CAGTACGCCT TTCAGGACAA AAATAACCTT TACCTGGTCA TGGAATATCA GCCTGGAGGG 
GATTTGCTGT CGCTTCTGAA CAGATACGAG GACCAATTAG ATGAGAGCAT GATCCAGTTT 
TACCTTGCTG AGCTGATTTT GGCTGTCCAC AGCGTGCACC AGATGGGATA TGTGCATCGA 
GACATCAAGC CCGAGAACAT CCTCATCGAC CGGACGGGAC ACATCAAGCT GGTGGATTTT 
GGATCAGCCG CTAAGATGAA TTCAAATAAG GTGGATGCCA AACTCCCCAT TGGGACCCCG 
GATTACATGG CTCCGGAAGT GTTGACCGTG ATGAACGAGG ACCGAAGGGG CACATACGGC 
TTGGACTGTG ACTGGTGGTC TGTCGGAGTT GTTGCTTATG AGATGGTTTA TGGGAAGACC 
CCATTCACAG AGGGAACCTC CGCCCGGACC TTCAACAACA TCATGAACTT CCAGCGGTTT 
TTGAAGTTCC CAGATGACCC CAAAGTTAGC AGTGAGCTCC TTGATCTGCT TCAGAGTCTG 
CTGTGTGTCC AGAAAGAGAG ACTGAAGTTC GAGGGTCTCT GCTGCCACCC TTTCTTTGCC 
AGAACGGACT GGAACAACAT CCGTAACTCT CCTCCCCCCT TCGTCCCCAC CCTCAAGTCT 
GACGATGACA CCTCCAATTT TGATGAACCA GAGAAGAATT CGTGGGTTTC ATCCTCTGTG 
TGCCAGCTGA GCCCCTCGGG CTTCTCAGGC GAAGAGCTGC CGTTTGTGGG ATTTTCGTAC 
AGCAAGGCAC TGGGGTATCT TGGTAGATCT GAGTCTGTCG TGTCGAGTCT GGACTCCCCT 
GCCAAGGTTA GCTCCATGGA AAAGAAACTT CTCATCAAAA GCAAAGAGCT CCAAGACTCC 
CAGGACAAGT GTCACAAGAT GGAGCAGGAA ATGACCCGGT TACATCGCAG AGTGTCAGAG 
GTGGAGGCTG TGCTTAGTCA GAAGGAGGTG GAGCTGAAGG CCTCTGAGAC TCAGAGATCC 
CTCCTGGAGC AGGACCTTGC TACCTACATC ACAGAATGCA GTAGCTTAAA GCGAAGTTTG 
GAGCAAGCGC GGATGGAGGT GTCCCAGGAG GATGACAAAG CTCTGCAGCT TCTCCACGAC 
ATCCGAGAGC AGAGCCGGAA GCTCCAGGAG ATCAAGGAGC AGGAGTACCA GGCTCAGGTG 
GAGGAGATGA GGCTGATGAT GAATCAGCTG GAAGAAGACC TGGTGTCAGC CCGCAGACGC 
AGCGATCTCT ACGAGTCTGA GCTGAGGGAG TCTCGGCTTG CCGCCGAGGA ATTCAAGCGG 
AAGGCAAACG AATGTCAGCA CAAACTGATG AAGGTAGTCA GCCACCCTCC AAGAGGGGAC 
TCCGGGGGCA CGGCCCTGGA TGATCTTCAC AAGATGCAAG GCCACGCTGG CTTAACGAGT 
GCTAAGGACC AAGGGAAGCC TGAAGTGGGA GAATATTCCA AACTGGAGAA GATCAATGCT 
GAGCAGCAGC TGAAGATCCA GGAGCTCCAG GAGAAGCTGG AGAAGGCTGT AAAAGCCAGC 
ACAGAGGCCA CCGAGCTCCT GCAGAATATC CGCCAGGCAA AGGAGCGAGC AGAGCGGGAG 
CTAGAGAAGC TACACAACCG GGAAGACTCC TCCGAGGGCA TCAAAAAGAA GCTGGTGGAA 
GCCGAGGAAC GCCGCCACTC CCTGGAGAAC AAGGTAAAGA GACTAGAGAC CATGGAGCGT 
AGAGAGAACA GACTGAAGGA TGACATCCAG ACAAAGTCCG AACAGATCCA GCAGATGGCT 
GATAAAATTC TGGAGCTGGA GGAGAAACAT CGGGAGGCTC AGGTCTCAGC TCAACATCTA 
GAAGTACACT TGAAACAGAA AGAACAGCAC TACGAGGAAA AGATCAAGGT ATTGGACAAT 
CAGATAAAGA AGGACCTGGC CGACAAGGAG AGCCTGGAGA ACATGATGCA GAGACACGAG 
GAGGAGGCCC ACGAGAAGGG CAAGATCCTC AGCGAGCAGA AGGCGATGAT CAACGCGATG 
GATTCCAAGA TCCGATCCCT GGAGCAGAGG ATCGTGGAGC TGTCGGAAGC CAACAAGCTT 
GCGGCAAACA GCAGTCTCTT CACCCAGAGG AACATGAAGG CCCAGGAAGA GATGATCTCA 
GAACTCAGGC AGCAGAAATT TTACCTGGAG ACGCAGGCCG GAAAGCTGGA GGCCCAGAAC 
CGAAAGCTGG AAGAGCAACT GGAGAAGATC AGCCACCAAG ATCACAGTGA CAAGAGTCGG 
CTGCTGGAGC TGGAAACAAG GCTGAGGGAG GTCAGCCTGG AGCACGAGGA GCAGAAGCTG 
GAGCTGAAGC GGCAGCTCAC GGAGCTGCAG CTGTCCCTGC AGGAGCGCGA GTCCCAGCTG 
ACGGCCCTGC AGGCCGCCCG GGCAGCGCTG GAGAGCCAGC TCCGCCAGGC GAAGACAGAG 
CTGGAGGAGA CAACCGCGGA AGCGGAGGAG GAGATCCAGG CGCTCACGGC ACATCGAGAT 
GAGATCCAGC GCAAATTCGA TGCCCTTCGC AACAGCTGCA CGGTCATCAC CGACCTGGAG 
GAGCAGCTGA ACCAGCTCAC CGAGGACAAC GCCGAGCTCA ACAACCAAAA CTTCTACCTG 
TCCAAACAAC TCGATGAGGC TTCCGGGGCC AATGACGAGA TTGTGCAGCT GCGGAGTGAG 
GTGGACCATC TCCGCCGTGA GATCACGGAG CGGGAGATGC AGCTCACCAG CCAGAAGCAA 
GCACAACTCA GTGCTCCTGA TTTGCAGACG ATGGAGGCTC TGAAGACGAC ATGCACCATG 
CTGGAGGAGC AGGTCCTGGA CCTGGAGGCC CTGAACGACG AGCTGCTGGA GAAGGAGCGC 
CAGTGGGAGG CCTGGCGGAG CGTCCTCGGC GACGAGAAGT CCCAGTTCGA GTGTCGAGTT 
CGAGAGCTAC AGAGGATGCT GGACACCGAG AAGCAGAGCA GGGCTAGGGC CGATCAGCGG 
ATCACCGAGT CGCGCCAGGT GGTGGAGTTG GCGGTGAAGG AACACAAGGC CGAGATTCTT 
GCTCTGCAGC AGGCTCTCAA GGAGCAGAAG CTCAAAGCCG AGAGCCTGTC GGACAAGCTC 
AACGACCTGG AGAAGAAGCA CGCCATGCTG GAGATGAACG CCCGGAGCTT ACAGCAGAAA 
CTAGAGACAG AGCGGGAGCT CAAACAGAGG CTTCTGGAGG AGCAAGCCAA ATTGCAGCAG 
CAGATGGACC TGCAGAAGAA CCACATCTTC AGACTGACGC AAGGGCTGCA GGAGGCGCTG 
GACCGGGCGG ATCTGCTGAA GACAGAAAGG AGCGACCTGG AGTACCAGCT GGAAAACATT 
CAGGTTCTCT ACTCTCACGA GAAAGTGAAA ATGGAAGGCA CAATCTCTCA GCAAACCAAA 
CTCATTGATT TCCTGCAAGC CAAAATGGAC CAGCCTGCTA AAAAGAAAAA GGGTTTATTT 
AGTCGACGGA AAGAGGACCC TGCTTTGCCC ACACAGGTTC CTCTGCAGTA CAATGAGCTG 
AAGCTAGCCC TGGAAAAGGA GAAAGCCCGA TGCGCGGAGC TGGAGGAGGC CCTTCAGAAG 
ACCCGCATCG AACTCCGCTC TGCGCGGGAG GAAGCTGCCC ACCGCAAAGC CACGGACCAC 
CCGCACCCGT CTACGCCAGC CACTGCGAGG CAGCAGATCG CCATGTCTGC CATTGTGCGG 
TCGCCCGAGC ACCAGCCCAG TGCCATGAGC CTGCTTGCCC CACCATCCAG CCGCAGAAAG 
GAGTCGTCAA CTCCAGAGGA ATTCAGCCGG CGTCTGAAAG AGCGCATGCA CCACAACATC 
CCTCACCGGT TTAATGTGGG CCTGAACATG AGAGCCACCA AGTGCGCCGT GTGTCTGGAT 
ACTGTGCACT TTGGACGCCA GGCATCCAAA TGCCTCGAAT GTCAAGTGAT GTGTCACCCC 
AAATGCTCCA CCTGCTTGCC CGCTACCTGT GGCCTGCCAG CTGAATATGC CACACACTTC 
ACTGAGGCCT TCTGCCGGGA CAAAATGAAC TCCCCGGGGC TCCAGAGCAA GGAGCCTGGC 
AGCAGCTTGC ACCTGGAAGG GTGGATGAAA GTGCCCAGAA ATAACAAACG GGGACAGCAA 
GGCTGGGACA GGAAGTACAT TGTTCTGGAG GGGTCAAAAG TCCTCATCTA TGACAATGAA 
GCCAGAGAAG CTGGACAGAG GCCGGTGGAA GAATTTGAGC TGTGCCTTCC TGACGGGGAC 
GTATCTATTC ATGGTGCCGT TGGTGCTTCA GAACTTGCAA ATACCGCCAA AGCAGATGTC 
CCATACATCC TGAAGATGGA GTCTCACCCA CACACCACCT GCTGGCCTGG GAGAACCCTC 
TACTTGCTAG CACCCAGCTT CCCCGACAAG CAGCGCTGGG TCACCGCCTT AGAATCTGTC 
GTCGCAGGTG GGAGAGTTTC TAGGGAAAAG GCCGAAGCCG ATGCTGCACG TGACCATACT 
TCGAGTGAGC ATCAGCCAGT GTGGGTTGAG AAATTACTTG GAAACTCTCT GCTGAAACTG 
GAGGGCGATG ACCGGCTTGA CATGAACTGC ACCCTGCCCT TCAGTGACCA GGTAGTGCTG 
GTGGGCACGG AGGAAGGCCT CTACGCCCTG AATGTCTTGA AAAACTCCTT AACCCACATC 
CCAGGGATTG GCGCAGTCTT CCAAATTTAC ATCATCAAGG ACCTGGAGAA GCTGCTCATG 
ATAGCAGGGG AAGAGCGGGC TCTGTGCCTG GTGGACGTGA AGAAGGTGAA GCAGTCCCTG 
GCGCAGTCAC ACCTGCCTGC CCAGCCCGAC GTCTCCCCCA ACATATTCGA AGCCGTCAAA 
GGCTGCCACT TGTTCGCTGC TGGCAAGATC GAGAACAGCC TGTGCATCTG CGCCGCTATG 
CCAAGCAAAG TCGTCATCCT CCGCTACAAT GACAACCTCA GCAAGTACTG CATCCGCAAG 
GAGATCGAGA CCTCAGAGCC CTGCAGCTGT ATCCACTTCA CCAACTACAG CATCCTCATT 
GGGACCAACA AATTCTATGA GATCGACATG AAGCAGTACA CGCTTGACGA GTTCCTGGAC 
AAGAACGACC ATTCCTTGGC ACCTGCTGTG TTCGCCTCCT CGTCCAACAG CTTCCCTGTC 
TCCATTGTGC AGGCGAACAG CGCCGGGCAG CGAGAAGAAT ACCTGCTGTG CTTCCACGAA 
TTTGGGGTGT TCGTGGATTC TTACGGAAGA CGTAGCCGCA CAGATGATCT CAAGTGGAGT 
CGCTTACCTC TGGCCTTCGC CTACAGAGAA CCTTATCTGT TTGTGACTCA CTTCAACTCC 
CTGGAAGTCA TTGAGATCCA GGCCAGATCC TCACTGGGGA GCCCTGCCCG AGCATATCTG 
GAAATTCCAA ACCCTCGCTA CCTGGGCCCC GCGATTTCCT CCGGAGCGAT TTACCTGGCC 
TCCTCATACC AGGACAAGTT AAGGGTCATA TGCTGCAAAG GAAACCTCGT GAAGGAGTCA 
GGCACTGAGC AGCACCGGGT GCCCTCCACC TCCCGCAGCA GCCCCAACAA GCGAGGCCCA 
CCAACATACA ACGAGCACAT CACCAAACGC GTGGCCTCCA GCCCGGCGCC ACCGGAAGGC 
CCCAGCCACC CCCGAGAGCC AAGCACACCG CACCGCTACC GAGACAGAGA GGGTCGGACA 
GAGCTGCGCA GGGACAAGTC TCCAGGCCGC CCTCTGGAGC GGGAGAAGTC CCCAGGCCGA 
ATGCTCAGCA CTAGGAGAGA GCGGTCCCCA GGGAGACTGT TTGAAGACAG CAGCAGGGGC 
CGGCTGCCTG CAGGAGCAGT GAGGACCCCA CTGTCCCAGG TTAACAAGGT CTGGGACCAG 
TCTTCAGTAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cit ( XM_006530131.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006530194.1 Mus musculus citron (Cit), transcript variant X2, mRNA.

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CloneID	OMu35774
Clone ID Related Accession (Same CDS sequence)	XM_006530130.3 , XM_006530132.3 , XM_006530133.3 , XM_006530130.4 , XM_006530131.2 , XM_006530131.1 , XM_006530133.4 , XM_006530132.4
Accession Version	XM_006530131.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6372bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	citron Rho-interacting kinase isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000071.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTGAAGT TCAAGTATGG TGTGCGGAAC CCGCCGGAGG CCAGTGCCTC CGAGCCCATT 
GCCAGTCGGG CCTCCAGGCT AAATCTCTTC TTCCAGGGGA AACCGCCCCT CATGACTCAA 
CAGCAGATGT CTGCTCTTTC CCGGGAAGGG ATGCTAGACG CCCTCTTCGC TCTCTTTGAA 
GAGTGCAGCC AACCCGCCCT GATGAAGATG AAGCACGTGA GCAGCTTTGT CCAGAAGTAT 
TCCGACACCA TAGCCGAGTT GCGGGAGCTG CAGCCGTCGG CGAGAGACTT CGAAGTTCGA 
AGCCTTGTGG GCTGTGGTCA CTTCGCTGAA GTGCAGGTGG TTAGAGAGAA GGCGACCGGG 
GACGTCTATG CCATGAAAAT CATGAAGAAG AAGGCTTTGC TGGCCCAGGA ACAGGTTTCA 
TTTTTCGAGG AGGAGAGGAA CATATTATCT CGGAGCACGA GTCCTTGGAT CCCCCAGTTA 
CAGTACGCCT TTCAGGACAA AAATAACCTT TACCTGGTCA TGGAATATCA GCCTGGAGGG 
GATTTGCTGT CGCTTCTGAA CAGATACGAG GACCAATTAG ATGAGAGCAT GATCCAGTTT 
TACCTTGCTG AGCTGATTTT GGCTGTCCAC AGCGTGCACC AGATGGGATA TGTGCATCGA 
GACATCAAGC CCGAGAACAT CCTCATCGAC CGGACGGGAC ACATCAAGCT GGTGGATTTT 
GGATCAGCCG CTAAGATGAA TTCAAATAAG GTGGATGCCA AACTCCCCAT TGGGACCCCG 
GATTACATGG CTCCGGAAGT GTTGACCGTG ATGAACGAGG ACCGAAGGGG CACATACGGC 
TTGGACTGTG ACTGGTGGTC TGTCGGAGTT GTTGCTTATG AGATGGTTTA TGGGAAGACC 
CCATTCACAG AGGGAACCTC CGCCCGGACC TTCAACAACA TCATGAACTT CCAGCGGTTT 
TTGAAGTTCC CAGATGACCC CAAAGTTAGC AGTGAGCTCC TTGATCTGCT TCAGAGTCTG 
CTGTGTGTCC AGAAAGAGAG ACTGAAGTTC GAGGGTCTCT GCTGCCACCC TTTCTTTGCC 
AGAACGGACT GGAACAACAT CCGTAACTCT CCTCCCCCCT TCGTCCCCAC CCTCAAGTCT 
GACGATGACA CCTCCAATTT TGATGAACCA GAGAAGAATT CGTGGGTTTC ATCCTCTGTG 
TGCCAGCTGA GCCCCTCGGG CTTCTCAGGC GAAGAGCTGC CGTTTGTGGG ATTTTCGTAC 
AGCAAGGCAC TGGGGTATCT TGGTAGATCT GAGTCTGTCG TGTCGAGTCT GGACTCCCCT 
GCCAAGGTTA GCTCCATGGA AAAGAAACTT CTCATCAAAA GCAAAGAGCT CCAAGACTCC 
CAGGACAAGT GTCACAAGAT GGAGCAGGAA ATGACCCGGT TACATCGCAG AGTGTCAGAG 
GTGGAGGCTG TGCTTAGTCA GAAGGAGGTG GAGCTGAAGG CCTCTGAGAC TCAGAGATCC 
CTCCTGGAGC AGGACCTTGC TACCTACATC ACAGAATGCA GTAGCTTAAA GCGAAGTTTG 
GAGCAAGCGC GGATGGAGGT GTCCCAGGAG GATGACAAAG CTCTGCAGCT TCTCCACGAC 
ATCCGAGAGC AGAGCCGGAA GCTCCAGGAG ATCAAGGAGC AGGAGTACCA GGCTCAGGTG 
GAGGAGATGA GGCTGATGAT GAATCAGCTG GAAGAAGACC TGGTGTCAGC CCGCAGACGC 
AGCGATCTCT ACGAGTCTGA GCTGAGGGAG TCTCGGCTTG CCGCCGAGGA ATTCAAGCGG 
AAGGCAAACG AATGTCAGCA CAAACTGATG AAGGTAGTCA GCCACCCTCC AAGAGGGGAC 
TCCGGGGGCA CGGCCCTGGA TGATCTTCAC AAGATGCAAG GCCACGCTGG CTTAACGAGT 
GCTAAGGACC AAGGGAAGCC TGAAGTGGGA GAATATTCCA AACTGGAGAA GATCAATGCT 
GAGCAGCAGC TGAAGATCCA GGAGCTCCAG GAGAAGCTGG AGAAGGCTGT AAAAGCCAGC 
ACAGAGGCCA CCGAGCTCCT GCAGAATATC CGCCAGGCAA AGGAGCGAGC AGAGCGGGAG 
CTAGAGAAGC TACACAACCG GGAAGACTCC TCCGAGGGCA TCAAAAAGAA GCTGGTGGAA 
GCCGAGGAAC GCCGCCACTC CCTGGAGAAC AAGGTAAAGA GACTAGAGAC CATGGAGCGT 
AGAGAGAACA GACTGAAGGA TGACATCCAG ACAAAGTCCG AACAGATCCA GCAGATGGCT 
GATAAAATTC TGGAGCTGGA GGAGAAACAT CGGGAGGCTC AGGTCTCAGC TCAACATCTA 
GAAGTACACT TGAAACAGAA AGAACAGCAC TACGAGGAAA AGATCAAGGT ATTGGACAAT 
CAGATAAAGA AGGACCTGGC CGACAAGGAG AGCCTGGAGA ACATGATGCA GAGACACGAG 
GAGGAGGCCC ACGAGAAGGG CAAGATCCTC AGCGAGCAGA AGGCGATGAT CAACGCGATG 
GATTCCAAGA TCCGATCCCT GGAGCAGAGG ATCGTGGAGC TGTCGGAAGC CAACAAGCTT 
GCGGCAAACA GCAGTCTCTT CACCCAGAGG AACATGAAGG CCCAGGAAGA GATGATCTCA 
GAACTCAGGC AGCAGAAATT TTACCTGGAG ACGCAGGCCG GAAAGCTGGA GGCCCAGAAC 
CGAAAGCTGG AAGAGCAACT GGAGAAGATC AGCCACCAAG ATCACAGTGA CAAGAGTCGG 
CTGCTGGAGC TGGAAACAAG GCTGAGGGAG GTCAGCCTGG AGCACGAGGA GCAGAAGCTG 
GAGCTGAAGC GGCAGCTCAC GGAGCTGCAG CTGTCCCTGC AGGAGCGCGA GTCCCAGCTG 
ACGGCCCTGC AGGCCGCCCG GGCAGCGCTG GAGAGCCAGC TCCGCCAGGC GAAGACAGAG 
CTGGAGGAGA CAACCGCGGA AGCGGAGGAG GAGATCCAGG CGCTCACGGC ACATCGAGAT 
GAGATCCAGC GCAAATTCGA TGCCCTTCGC AACAGCTGCA CGGTCATCAC CGACCTGGAG 
GAGCAGCTGA ACCAGCTCAC CGAGGACAAC GCCGAGCTCA ACAACCAAAA CTTCTACCTG 
TCCAAACAAC TCGATGAGGC TTCCGGGGCC AATGACGAGA TTGTGCAGCT GCGGAGTGAG 
GTGGACCATC TCCGCCGTGA GATCACGGAG CGGGAGATGC AGCTCACCAG CCAGAAGCAA 
GCACAACTCA GTGCTCCTGA TTTGCAGACG ATGGAGGCTC TGAAGACGAC ATGCACCATG 
CTGGAGGAGC AGGTCCTGGA CCTGGAGGCC CTGAACGACG AGCTGCTGGA GAAGGAGCGC 
CAGTGGGAGG CCTGGCGGAG CGTCCTCGGC GACGAGAAGT CCCAGTTCGA GTGTCGAGTT 
CGAGAGCTAC AGAGGATGCT GGACACCGAG AAGCAGAGCA GGGCTAGGGC CGATCAGCGG 
ATCACCGAGT CGCGCCAGGT GGTGGAGTTG GCGGTGAAGG AACACAAGGC CGAGATTCTT 
GCTCTGCAGC AGGCTCTCAA GGAGCAGAAG CTCAAAGCCG AGAGCCTGTC GGACAAGCTC 
AACGACCTGG AGAAGAAGCA CGCCATGCTG GAGATGAACG CCCGGAGCTT ACAGCAGAAA 
CTAGAGACAG AGCGGGAGCT CAAACAGAGG CTTCTGGAGG AGCAAGCCAA ATTGCAGCAG 
CAGATGGACC TGCAGAAGAA CCACATCTTC AGACTGACGC AAGGGCTGCA GGAGGCGCTG 
GACCGGGCGG ATCTGCTGAA GACAGAAAGG AGCGACCTGG AGTACCAGCT GGAAAACATT 
CAGGTTCTCT ACTCTCACGA GAAAGTGAAA ATGGAAGGCA CAATCTCTCA GCAAACCAAA 
CTCATTGATT TCCTGCAAGC CAAAATGGAC CAGCCTGCTA AAAAGAAAAA GGGTTTATTT 
AGTCGACGGA AAGAGGACCC TGCTTTGCCC ACACAGGTTC CTCTGCAGTA CAATGAGCTG 
AAGCTAGCCC TGGAAAAGGA GAAAGCCCGA TGCGCGGAGC TGGAGGAGGC CCTTCAGAAG 
ACCCGCATCG AACTCCGCTC TGCGCGGGAG GAAGCTGCCC ACCGCAAAGC CACGGACCAC 
CCGCACCCGT CTACGCCAGC CACTGCGAGG CAGCAGATCG CCATGTCTGC CATTGTGCGG 
TCGCCCGAGC ACCAGCCCAG TGCCATGAGC CTGCTTGCCC CACCATCCAG CCGCAGAAAG 
GAGTCGTCAA CTCCAGAGGA ATTCAGCCGG CGTCTGAAAG AGCGCATGCA CCACAACATC 
CCTCACCGGT TTAATGTGGG CCTGAACATG AGAGCCACCA AGTGCGCCGT GTGTCTGGAT 
ACTGTGCACT TTGGACGCCA GGCATCCAAA TGCCTCGAAT GTCAAGTGAT GTGTCACCCC 
AAATGCTCCA CCTGCTTGCC CGCTACCTGT GGCCTGCCAG CTGAATATGC CACACACTTC 
ACTGAGGCCT TCTGCCGGGA CAAAATGAAC TCCCCGGGGC TCCAGAGCAA GGAGCCTGGC 
AGCAGCTTGC ACCTGGAAGG GTGGATGAAA GTGCCCAGAA ATAACAAACG GGGACAGCAA 
GGCTGGGACA GGAAGTACAT TGTTCTGGAG GGGTCAAAAG TCCTCATCTA TGACAATGAA 
GCCAGAGAAG CTGGACAGAG GCCGGTGGAA GAATTTGAGC TGTGCCTTCC TGACGGGGAC 
GTATCTATTC ATGGTGCCGT TGGTGCTTCA GAACTTGCAA ATACCGCCAA AGCAGATGTC 
CCATACATCC TGAAGATGGA GTCTCACCCA CACACCACCT GCTGGCCTGG GAGAACCCTC 
TACTTGCTAG CACCCAGCTT CCCCGACAAG CAGCGCTGGG TCACCGCCTT AGAATCTGTC 
GTCGCAGGTG GGAGAGTTTC TAGGGAAAAG GCCGAAGCCG ATGCTGCACG TGACCATACT 
TCGAGTGAGC ATCAGCCAGT GTGGGTTGAG AAATTACTTG GAAACTCTCT GCTGAAACTG 
GAGGGCGATG ACCGGCTTGA CATGAACTGC ACCCTGCCCT TCAGTGACCA GGTAGTGCTG 
GTGGGCACGG AGGAAGGCCT CTACGCCCTG AATGTCTTGA AAAACTCCTT AACCCACATC 
CCAGGGATTG GCGCAGTCTT CCAAATTTAC ATCATCAAGG ACCTGGAGAA GCTGCTCATG 
ATAGCAGGGG AAGAGCGGGC TCTGTGCCTG GTGGACGTGA AGAAGGTGAA GCAGTCCCTG 
GCGCAGTCAC ACCTGCCTGC CCAGCCCGAC GTCTCCCCCA ACATATTCGA AGCCGTCAAA 
GGCTGCCACT TGTTCGCTGC TGGCAAGATC GAGAACAGCC TGTGCATCTG CGCCGCTATG 
CCAAGCAAAG TCGTCATCCT CCGCTACAAT GACAACCTCA GCAAGTACTG CATCCGCAAG 
GAGATCGAGA CCTCAGAGCC CTGCAGCTGT ATCCACTTCA CCAACTACAG CATCCTCATT 
GGGACCAACA AATTCTATGA GATCGACATG AAGCAGTACA CGCTTGACGA GTTCCTGGAC 
AAGAACGACC ATTCCTTGGC ACCTGCTGTG TTCGCCTCCT CGTCCAACAG CTTCCCTGTC 
TCCATTGTGC AGGCGAACAG CGCCGGGCAG CGAGAAGAAT ACCTGCTGTG CTTCCACGAA 
TTTGGGGTGT TCGTGGATTC TTACGGAAGA CGTAGCCGCA CAGATGATCT CAAGTGGAGT 
CGCTTACCTC TGGCCTTCGC CTACAGAGAA CCTTATCTGT TTGTGACTCA CTTCAACTCC 
CTGGAAGTCA TTGAGATCCA GGCCAGATCC TCACTGGGGA GCCCTGCCCG AGCATATCTG 
GAAATTCCAA ACCCTCGCTA CCTGGGCCCC GCGATTTCCT CCGGAGCGAT TTACCTGGCC 
TCCTCATACC AGGACAAGTT AAGGGTCATA TGCTGCAAAG GAAACCTCGT GAAGGAGTCA 
GGCACTGAGC AGCACCGGGT GCCCTCCACC TCCCGCAGCA GCCCCAACAA GCGAGGCCCA 
CCAACATACA ACGAGCACAT CACCAAACGC GTGGCCTCCA GCCCGGCGCC ACCGGAAGGC 
CCCAGCCACC CCCGAGAGCC AAGCACACCG CACCGCTACC GAGACAGAGA GGGTCGGACA 
GAGCTGCGCA GGGACAAGTC TCCAGGCCGC CCTCTGGAGC GGGAGAAGTC CCCAGGCCGA 
ATGCTCAGCA CTAGGAGAGA GCGGTCCCCA GGGAGACTGT TTGAAGACAG CAGCAGGGGC 
CGGCTGCCTG CAGGAGCAGT GAGGACCCCA CTGTCCCAGG TTAACAAGGT CTGGGACCAG 
TCTTCAGTAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006530194.1
CDS	103..6474
Translation	MLKFKYGVRNPPEASASEPIASRASRLNLFFQGKPPLMTQQQMSALSREGMLDALFALFEECSQPALMKMKHVSSFVQKYSDTIAELRELQPSARDFEVRSLVGCGHFAEVQVVREKATGDVYAMKIMKKKALLAQEQVSFFEEERNILSRSTSPWIPQLQYAFQDKNNLYLVMEYQPGGDLLSLLNRYEDQLDESMIQFYLAELILAVHSVHQMGYVHRDIKPENILIDRTGHIKLVDFGSAAKMNSNKVDAKLPIGTPDYMAPEVLTVMNEDRRGTYGLDCDWWSVGVVAYEMVYGKTPFTEGTSARTFNNIMNFQRFLKFPDDPKVSSELLDLLQSLLCVQKERLKFEGLCCHPFFARTDWNNIRNSPPPFVPTLKSDDDTSNFDEPEKNSWVSSSVCQLSPSGFSGEELPFVGFSYSKALGYLGRSESVVSSLDSPAKVSSMEKKLLIKSKELQDSQDKCHKMEQEMTRLHRRVSEVEAVLSQKEVELKASETQRSLLEQDLATYITECSSLKRSLEQARMEVSQEDDKALQLLHDIREQSRKLQEIKEQEYQAQVEEMRLMMNQLEEDLVSARRRSDLYESELRESRLAAEEFKRKANECQHKLMKVVSHPPRGDSGGTALDDLHKMQGHAGLTSAKDQGKPEVGEYSKLEKINAEQQLKIQELQEKLEKAVKASTEATELLQNIRQAKERAERELEKLHNREDSSEGIKKKLVEAEERRHSLENKVKRLETMERRENRLKDDIQTKSEQIQQMADKILELEEKHREAQVSAQHLEVHLKQKEQHYEEKIKVLDNQIKKDLADKESLENMMQRHEEEAHEKGKILSEQKAMINAMDSKIRSLEQRIVELSEANKLAANSSLFTQRNMKAQEEMISELRQQKFYLETQAGKLEAQNRKLEEQLEKISHQDHSDKSRLLELETRLREVSLEHEEQKLELKRQLTELQLSLQERESQLTALQAARAALESQLRQAKTELEETTAEAEEEIQALTAHRDEIQRKFDALRNSCTVITDLEEQLNQLTEDNAELNNQNFYLSKQLDEASGANDEIVQLRSEVDHLRREITEREMQLTSQKQAQLSAPDLQTMEALKTTCTMLEEQVLDLEALNDELLEKERQWEAWRSVLGDEKSQFECRVRELQRMLDTEKQSRARADQRITESRQVVELAVKEHKAEILALQQALKEQKLKAESLSDKLNDLEKKHAMLEMNARSLQQKLETERELKQRLLEEQAKLQQQMDLQKNHIFRLTQGLQEALDRADLLKTERSDLEYQLENIQVLYSHEKVKMEGTISQQTKLIDFLQAKMDQPAKKKKGLFSRRKEDPALPTQVPLQYNELKLALEKEKARCAELEEALQKTRIELRSAREEAAHRKATDHPHPSTPATARQQIAMSAIVRSPEHQPSAMSLLAPPSSRRKESSTPEEFSRRLKERMHHNIPHRFNVGLNMRATKCAVCLDTVHFGRQASKCLECQVMCHPKCSTCLPATCGLPAEYATHFTEAFCRDKMNSPGLQSKEPGSSLHLEGWMKVPRNNKRGQQGWDRKYIVLEGSKVLIYDNEAREAGQRPVEEFELCLPDGDVSIHGAVGASELANTAKADVPYILKMESHPHTTCWPGRTLYLLAPSFPDKQRWVTALESVVAGGRVSREKAEADAARDHTSSEHQPVWVEKLLGNSLLKLEGDDRLDMNCTLPFSDQVVLVGTEEGLYALNVLKNSLTHIPGIGAVFQIYIIKDLEKLLMIAGEERALCLVDVKKVKQSLAQSHLPAQPDVSPNIFEAVKGCHLFAAGKIENSLCICAAMPSKVVILRYNDNLSKYCIRKEIETSEPCSCIHFTNYSILIGTNKFYEIDMKQYTLDEFLDKNDHSLAPAVFASSSNSFPVSIVQANSAGQREEYLLCFHEFGVFVDSYGRRSRTDDLKWSRLPLAFAYREPYLFVTHFNSLEVIEIQARSSLGSPARAYLEIPNPRYLGPAISSGAIYLASSYQDKLRVICCKGNLVKESGTEQHRVPSTSRSSPNKRGPPTYNEHITKRVASSPAPPEGPSHPREPSTPHRYRDREGRTELRRDKSPGRPLEREKSPGRMLSTRRERSPGRLFEDSSRGRLPAGAVRTPLSQVNKVWDQSSV

Target ORF information:

RefSeq Version	XM_006530131.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus citron (Cit), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006530131.1

ORF Insert Sequence:

ATGTTGAAGT TCAAGTATGG TGTGCGGAAC CCGCCGGAGG CCAGTGCCTC CGAGCCCATT 
GCCAGTCGGG CCTCCAGGCT AAATCTCTTC TTCCAGGGGA AACCGCCCCT CATGACTCAA 
CAGCAGATGT CTGCTCTTTC CCGGGAAGGG ATGCTAGACG CCCTCTTCGC TCTCTTTGAA 
GAGTGCAGCC AACCCGCCCT GATGAAGATG AAGCACGTGA GCAGCTTTGT CCAGAAGTAT 
TCCGACACCA TAGCCGAGTT GCGGGAGCTG CAGCCGTCGG CGAGAGACTT CGAAGTTCGA 
AGCCTTGTGG GCTGTGGTCA CTTCGCTGAA GTGCAGGTGG TTAGAGAGAA GGCGACCGGG 
GACGTCTATG CCATGAAAAT CATGAAGAAG AAGGCTTTGC TGGCCCAGGA ACAGGTTTCA 
TTTTTCGAGG AGGAGAGGAA CATATTATCT CGGAGCACGA GTCCTTGGAT CCCCCAGTTA 
CAGTACGCCT TTCAGGACAA AAATAACCTT TACCTGGTCA TGGAATATCA GCCTGGAGGG 
GATTTGCTGT CGCTTCTGAA CAGATACGAG GACCAATTAG ATGAGAGCAT GATCCAGTTT 
TACCTTGCTG AGCTGATTTT GGCTGTCCAC AGCGTGCACC AGATGGGATA TGTGCATCGA 
GACATCAAGC CCGAGAACAT CCTCATCGAC CGGACGGGAC ACATCAAGCT GGTGGATTTT 
GGATCAGCCG CTAAGATGAA TTCAAATAAG GTGGATGCCA AACTCCCCAT TGGGACCCCG 
GATTACATGG CTCCGGAAGT GTTGACCGTG ATGAACGAGG ACCGAAGGGG CACATACGGC 
TTGGACTGTG ACTGGTGGTC TGTCGGAGTT GTTGCTTATG AGATGGTTTA TGGGAAGACC 
CCATTCACAG AGGGAACCTC CGCCCGGACC TTCAACAACA TCATGAACTT CCAGCGGTTT 
TTGAAGTTCC CAGATGACCC CAAAGTTAGC AGTGAGCTCC TTGATCTGCT TCAGAGTCTG 
CTGTGTGTCC AGAAAGAGAG ACTGAAGTTC GAGGGTCTCT GCTGCCACCC TTTCTTTGCC 
AGAACGGACT GGAACAACAT CCGTAACTCT CCTCCCCCCT TCGTCCCCAC CCTCAAGTCT 
GACGATGACA CCTCCAATTT TGATGAACCA GAGAAGAATT CGTGGGTTTC ATCCTCTGTG 
TGCCAGCTGA GCCCCTCGGG CTTCTCAGGC GAAGAGCTGC CGTTTGTGGG ATTTTCGTAC 
AGCAAGGCAC TGGGGTATCT TGGTAGATCT GAGTCTGTCG TGTCGAGTCT GGACTCCCCT 
GCCAAGGTTA GCTCCATGGA AAAGAAACTT CTCATCAAAA GCAAAGAGCT CCAAGACTCC 
CAGGACAAGT GTCACAAGAT GGAGCAGGAA ATGACCCGGT TACATCGCAG AGTGTCAGAG 
GTGGAGGCTG TGCTTAGTCA GAAGGAGGTG GAGCTGAAGG CCTCTGAGAC TCAGAGATCC 
CTCCTGGAGC AGGACCTTGC TACCTACATC ACAGAATGCA GTAGCTTAAA GCGAAGTTTG 
GAGCAAGCGC GGATGGAGGT GTCCCAGGAG GATGACAAAG CTCTGCAGCT TCTCCACGAC 
ATCCGAGAGC AGAGCCGGAA GCTCCAGGAG ATCAAGGAGC AGGAGTACCA GGCTCAGGTG 
GAGGAGATGA GGCTGATGAT GAATCAGCTG GAAGAAGACC TGGTGTCAGC CCGCAGACGC 
AGCGATCTCT ACGAGTCTGA GCTGAGGGAG TCTCGGCTTG CCGCCGAGGA ATTCAAGCGG 
AAGGCAAACG AATGTCAGCA CAAACTGATG AAGGTAGTCA GCCACCCTCC AAGAGGGGAC 
TCCGGGGGCA CGGCCCTGGA TGATCTTCAC AAGATGCAAG GCCACGCTGG CTTAACGAGT 
GCTAAGGACC AAGGGAAGCC TGAAGTGGGA GAATATTCCA AACTGGAGAA GATCAATGCT 
GAGCAGCAGC TGAAGATCCA GGAGCTCCAG GAGAAGCTGG AGAAGGCTGT AAAAGCCAGC 
ACAGAGGCCA CCGAGCTCCT GCAGAATATC CGCCAGGCAA AGGAGCGAGC AGAGCGGGAG 
CTAGAGAAGC TACACAACCG GGAAGACTCC TCCGAGGGCA TCAAAAAGAA GCTGGTGGAA 
GCCGAGGAAC GCCGCCACTC CCTGGAGAAC AAGGTAAAGA GACTAGAGAC CATGGAGCGT 
AGAGAGAACA GACTGAAGGA TGACATCCAG ACAAAGTCCG AACAGATCCA GCAGATGGCT 
GATAAAATTC TGGAGCTGGA GGAGAAACAT CGGGAGGCTC AGGTCTCAGC TCAACATCTA 
GAAGTACACT TGAAACAGAA AGAACAGCAC TACGAGGAAA AGATCAAGGT ATTGGACAAT 
CAGATAAAGA AGGACCTGGC CGACAAGGAG AGCCTGGAGA ACATGATGCA GAGACACGAG 
GAGGAGGCCC ACGAGAAGGG CAAGATCCTC AGCGAGCAGA AGGCGATGAT CAACGCGATG 
GATTCCAAGA TCCGATCCCT GGAGCAGAGG ATCGTGGAGC TGTCGGAAGC CAACAAGCTT 
GCGGCAAACA GCAGTCTCTT CACCCAGAGG AACATGAAGG CCCAGGAAGA GATGATCTCA 
GAACTCAGGC AGCAGAAATT TTACCTGGAG ACGCAGGCCG GAAAGCTGGA GGCCCAGAAC 
CGAAAGCTGG AAGAGCAACT GGAGAAGATC AGCCACCAAG ATCACAGTGA CAAGAGTCGG 
CTGCTGGAGC TGGAAACAAG GCTGAGGGAG GTCAGCCTGG AGCACGAGGA GCAGAAGCTG 
GAGCTGAAGC GGCAGCTCAC GGAGCTGCAG CTGTCCCTGC AGGAGCGCGA GTCCCAGCTG 
ACGGCCCTGC AGGCCGCCCG GGCAGCGCTG GAGAGCCAGC TCCGCCAGGC GAAGACAGAG 
CTGGAGGAGA CAACCGCGGA AGCGGAGGAG GAGATCCAGG CGCTCACGGC ACATCGAGAT 
GAGATCCAGC GCAAATTCGA TGCCCTTCGC AACAGCTGCA CGGTCATCAC CGACCTGGAG 
GAGCAGCTGA ACCAGCTCAC CGAGGACAAC GCCGAGCTCA ACAACCAAAA CTTCTACCTG 
TCCAAACAAC TCGATGAGGC TTCCGGGGCC AATGACGAGA TTGTGCAGCT GCGGAGTGAG 
GTGGACCATC TCCGCCGTGA GATCACGGAG CGGGAGATGC AGCTCACCAG CCAGAAGCAA 
GCACAACTCA GTGCTCCTGA TTTGCAGACG ATGGAGGCTC TGAAGACGAC ATGCACCATG 
CTGGAGGAGC AGGTCCTGGA CCTGGAGGCC CTGAACGACG AGCTGCTGGA GAAGGAGCGC 
CAGTGGGAGG CCTGGCGGAG CGTCCTCGGC GACGAGAAGT CCCAGTTCGA GTGTCGAGTT 
CGAGAGCTAC AGAGGATGCT GGACACCGAG AAGCAGAGCA GGGCTAGGGC CGATCAGCGG 
ATCACCGAGT CGCGCCAGGT GGTGGAGTTG GCGGTGAAGG AACACAAGGC CGAGATTCTT 
GCTCTGCAGC AGGCTCTCAA GGAGCAGAAG CTCAAAGCCG AGAGCCTGTC GGACAAGCTC 
AACGACCTGG AGAAGAAGCA CGCCATGCTG GAGATGAACG CCCGGAGCTT ACAGCAGAAA 
CTAGAGACAG AGCGGGAGCT CAAACAGAGG CTTCTGGAGG AGCAAGCCAA ATTGCAGCAG 
CAGATGGACC TGCAGAAGAA CCACATCTTC AGACTGACGC AAGGGCTGCA GGAGGCGCTG 
GACCGGGCGG ATCTGCTGAA GACAGAAAGG AGCGACCTGG AGTACCAGCT GGAAAACATT 
CAGGTTCTCT ACTCTCACGA GAAAGTGAAA ATGGAAGGCA CAATCTCTCA GCAAACCAAA 
CTCATTGATT TCCTGCAAGC CAAAATGGAC CAGCCTGCTA AAAAGAAAAA GGGTTTATTT 
AGTCGACGGA AAGAGGACCC TGCTTTGCCC ACACAGGTTC CTCTGCAGTA CAATGAGCTG 
AAGCTAGCCC TGGAAAAGGA GAAAGCCCGA TGCGCGGAGC TGGAGGAGGC CCTTCAGAAG 
ACCCGCATCG AACTCCGCTC TGCGCGGGAG GAAGCTGCCC ACCGCAAAGC CACGGACCAC 
CCGCACCCGT CTACGCCAGC CACTGCGAGG CAGCAGATCG CCATGTCTGC CATTGTGCGG 
TCGCCCGAGC ACCAGCCCAG TGCCATGAGC CTGCTTGCCC CACCATCCAG CCGCAGAAAG 
GAGTCGTCAA CTCCAGAGGA ATTCAGCCGG CGTCTGAAAG AGCGCATGCA CCACAACATC 
CCTCACCGGT TTAATGTGGG CCTGAACATG AGAGCCACCA AGTGCGCCGT GTGTCTGGAT 
ACTGTGCACT TTGGACGCCA GGCATCCAAA TGCCTCGAAT GTCAAGTGAT GTGTCACCCC 
AAATGCTCCA CCTGCTTGCC CGCTACCTGT GGCCTGCCAG CTGAATATGC CACACACTTC 
ACTGAGGCCT TCTGCCGGGA CAAAATGAAC TCCCCGGGGC TCCAGAGCAA GGAGCCTGGC 
AGCAGCTTGC ACCTGGAAGG GTGGATGAAA GTGCCCAGAA ATAACAAACG GGGACAGCAA 
GGCTGGGACA GGAAGTACAT TGTTCTGGAG GGGTCAAAAG TCCTCATCTA TGACAATGAA 
GCCAGAGAAG CTGGACAGAG GCCGGTGGAA GAATTTGAGC TGTGCCTTCC TGACGGGGAC 
GTATCTATTC ATGGTGCCGT TGGTGCTTCA GAACTTGCAA ATACCGCCAA AGCAGATGTC 
CCATACATCC TGAAGATGGA GTCTCACCCA CACACCACCT GCTGGCCTGG GAGAACCCTC 
TACTTGCTAG CACCCAGCTT CCCCGACAAG CAGCGCTGGG TCACCGCCTT AGAATCTGTC 
GTCGCAGGTG GGAGAGTTTC TAGGGAAAAG GCCGAAGCCG ATGCTGCACG TGACCATACT 
TCGAGTGAGC ATCAGCCAGT GTGGGTTGAG AAATTACTTG GAAACTCTCT GCTGAAACTG 
GAGGGCGATG ACCGGCTTGA CATGAACTGC ACCCTGCCCT TCAGTGACCA GGTAGTGCTG 
GTGGGCACGG AGGAAGGCCT CTACGCCCTG AATGTCTTGA AAAACTCCTT AACCCACATC 
CCAGGGATTG GCGCAGTCTT CCAAATTTAC ATCATCAAGG ACCTGGAGAA GCTGCTCATG 
ATAGCAGGGG AAGAGCGGGC TCTGTGCCTG GTGGACGTGA AGAAGGTGAA GCAGTCCCTG 
GCGCAGTCAC ACCTGCCTGC CCAGCCCGAC GTCTCCCCCA ACATATTCGA AGCCGTCAAA 
GGCTGCCACT TGTTCGCTGC TGGCAAGATC GAGAACAGCC TGTGCATCTG CGCCGCTATG 
CCAAGCAAAG TCGTCATCCT CCGCTACAAT GACAACCTCA GCAAGTACTG CATCCGCAAG 
GAGATCGAGA CCTCAGAGCC CTGCAGCTGT ATCCACTTCA CCAACTACAG CATCCTCATT 
GGGACCAACA AATTCTATGA GATCGACATG AAGCAGTACA CGCTTGACGA GTTCCTGGAC 
AAGAACGACC ATTCCTTGGC ACCTGCTGTG TTCGCCTCCT CGTCCAACAG CTTCCCTGTC 
TCCATTGTGC AGGCGAACAG CGCCGGGCAG CGAGAAGAAT ACCTGCTGTG CTTCCACGAA 
TTTGGGGTGT TCGTGGATTC TTACGGAAGA CGTAGCCGCA CAGATGATCT CAAGTGGAGT 
CGCTTACCTC TGGCCTTCGC CTACAGAGAA CCTTATCTGT TTGTGACTCA CTTCAACTCC 
CTGGAAGTCA TTGAGATCCA GGCCAGATCC TCACTGGGGA GCCCTGCCCG AGCATATCTG 
GAAATTCCAA ACCCTCGCTA CCTGGGCCCC GCGATTTCCT CCGGAGCGAT TTACCTGGCC 
TCCTCATACC AGGACAAGTT AAGGGTCATA TGCTGCAAAG GAAACCTCGT GAAGGAGTCA 
GGCACTGAGC AGCACCGGGT GCCCTCCACC TCCCGCAGCA GCCCCAACAA GCGAGGCCCA 
CCAACATACA ACGAGCACAT CACCAAACGC GTGGCCTCCA GCCCGGCGCC ACCGGAAGGC 
CCCAGCCACC CCCGAGAGCC AAGCACACCG CACCGCTACC GAGACAGAGA GGGTCGGACA 
GAGCTGCGCA GGGACAAGTC TCCAGGCCGC CCTCTGGAGC GGGAGAAGTC CCCAGGCCGA 
ATGCTCAGCA CTAGGAGAGA GCGGTCCCCA GGGAGACTGT TTGAAGACAG CAGCAGGGGC 
CGGCTGCCTG CAGGAGCAGT GAGGACCCCA CTGTCCCAGG TTAACAAGGT CTGGGACCAG 
TCTTCAGTAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cit ( XM_006530134.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006530197.1 Mus musculus citron (Cit), transcript variant X5, mRNA.

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CloneID	OMu35775
Clone ID Related Accession (Same CDS sequence)	XM_006530134.3 , XM_006530134.4
Accession Version	XM_006530134.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6369bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	citron Rho-interacting kinase isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000071.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006530134.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTGAAGT TCAAGTATGG TGTGCGGAAC CCGCCGGAGG CCAGTGCCTC CGAGCCCATT 
GCCAGTCGGG CCTCCAGGCT AAATCTCTTC TTCCAGGGGA AACCGCCCCT CATGACTCAA 
CAGCAGATGT CTGCTCTTTC CCGGGAAGGG ATGCTAGACG CCCTCTTCGC TCTCTTTGAA 
GAGTGCAGCC AACCCGCCCT GATGAAGATG AAGCACGTGA GCAGCTTTGT CCAGAAGTAT 
TCCGACACCA TAGCCGAGTT GCGGGAGCTG CAGCCGTCGG CGAGAGACTT CGAAGTTCGA 
AGCCTTGTGG GCTGTGGTCA CTTCGCTGAA GTGCAGGTGG TTAGAGAGAA GGCGACCGGG 
GACGTCTATG CCATGAAAAT CATGAAGAAG AAGGCTTTGC TGGCCCAGGA ACAGGTTTCA 
TTTTTCGAGG AGGAGAGGAA CATATTATCT CGGAGCACGA GTCCTTGGAT CCCCCAGTTA 
CAGTACGCCT TTCAGGACAA AAATAACCTT TACCTGGTCA TGGAATATCA GCCTGGAGGG 
GATTTGCTGT CGCTTCTGAA CAGATACGAG GACCAATTAG ATGAGAGCAT GATCCAGTTT 
TACCTTGCTG AGCTGATTTT GGCTGTCCAC AGCGTGCACC AGATGGGATA TGTGCATCGA 
GACATCAAGC CCGAGAACAT CCTCATCGAC CGGACGGGAC ACATCAAGCT GGTGGATTTT 
GGATCAGCCG CTAAGATGAA TTCAAATAAG GTGGATGCCA AACTCCCCAT TGGGACCCCG 
GATTACATGG CTCCGGAAGT GTTGACCGTG ATGAACGAGG ACCGAAGGGG CACATACGGC 
TTGGACTGTG ACTGGTGGTC TGTCGGAGTT GTTGCTTATG AGATGGTTTA TGGGAAGACC 
CCATTCACAG AGGGAACCTC CGCCCGGACC TTCAACAACA TCATGAACTT CCAGCGGTTT 
TTGAAGTTCC CAGATGACCC CAAAGTTAGC AGTGAGCTCC TTGATCTGCT TCAGAGTCTG 
CTGTGTGTCC AGAAAGAGAG ACTGAAGTTC GAGGGTCTCT GCTGCCACCC TTTCTTTGCC 
AGAACGGACT GGAACAACAT CCGTAACTCT CCTCCCCCCT TCGTCCCCAC CCTCAAGTCT 
GACGATGACA CCTCCAATTT TGATGAACCA GAGAAGAATT CGTGGGTTTC ATCCTCTGTG 
TGCCAGCTGA GCCCCTCGGG CTTCTCAGGC GAAGAGCTGC CGTTTGTGGG ATTTTCGTAC 
AGCAAGGCAC TGGGGTATCT TGGTAGATCT GAGTCTGTCG TGTCGAGTCT GGACTCCCCT 
GCCAAGGTTA GCTCCATGGA AAAGAAACTT CTCATCAAAA GCAAAGAGCT CCAAGACTCC 
CAGGACAAGT GTCACAAGAT GGAGCAGGAA ATGACCCGGT TACATCGCAG AGTGTCAGAG 
GTGGAGGCTG TGCTTAGTCA GAAGGAGGTG GAGCTGAAGG CCTCTGAGAC TCAGAGATCC 
CTCCTGGAGC AGGACCTTGC TACCTACATC ACAGAATGCA GTAGCTTAAA GCGAAGTTTG 
GAGCAAGCGC GGATGGAGGT GTCCCAGGAG GATGACAAAG CTCTGCAGCT TCTCCACGAC 
ATCCGAGAGC AGAGCCGGAA GCTCCAGGAG ATCAAGGAGC AGGAGTACCA GGCTCAGGTG 
GAGGAGATGA GGCTGATGAT GAATCAGCTG GAAGAAGACC TGGTGTCAGC CCGCAGACGC 
AGCGATCTCT ACGAGTCTGA GCTGAGGGAG TCTCGGCTTG CCGCCGAGGA ATTCAAGCGG 
AAGGCAAACG AATGTCAGCA CAAACTGATG AAGGTAGTCA GCCACCCTCC AAGAGGGGAC 
TCCGGGGGCA CGGCCCTGGA TGATCTTCAC AAGATGCAAG GCCACGCTGG CTTAACGAGT 
GCTAAGGACC AAGGGAAGCC TGAAGTGGGA GAATATTCCA AACTGGAGAA GATCAATGCT 
GAGCAGCAGC TGAAGATCCA GGAGCTCCAG GAGAAGCTGG AGAAGGCTGT AAAAGCCAGC 
ACAGAGGCCA CCGAGCTCCT GCAGAATATC CGCCAGGCAA AGGAGCGAGC AGAGCGGGAG 
CTAGAGAAGC TACACAACCG GGAAGACTCC TCCGAGGGCA TCAAAAAGAA GCTGGTGGAA 
GCCGAGGAAC GCCGCCACTC CCTGGAGAAC AAGGTAAAGA GACTAGAGAC CATGGAGCGT 
AGAGAGAACA GACTGAAGGA TGACATCCAG ACAAAGTCCG AACAGATCCA GCAGATGGCT 
GATAAAATTC TGGAGCTGGA GGAGAAACAT CGGGAGGCTC AGGTCTCAGC TCAACATCTA 
GAAGTACACT TGAAACAGAA AGAACAGCAC TACGAGGAAA AGATCAAGGT ATTGGACAAT 
CAGATAAAGA AGGACCTGGC CGACAAGGAG AGCCTGGAGA ACATGATGCA GAGACACGAG 
GAGGAGGCCC ACGAGAAGGG CAAGATCCTC AGCGAGCAGA AGGCGATGAT CAACGCGATG 
GATTCCAAGA TCCGATCCCT GGAGCAGAGG ATCGTGGAGC TGTCGGAAGC CAACAAGCTT 
GCGGCAAACA GCAGTCTCTT CACCCAGAGG AACATGAAGG CCCAGGAAGA GATGATCTCA 
GAACTCAGGC AGCAGAAATT TTACCTGGAG ACGCAGGCCG GAAAGCTGGA GGCCCAGAAC 
CGAAAGCTGG AAGAGCAACT GGAGAAGATC AGCCACCAAG ATCACAGTGA CAAGAGTCGG 
CTGCTGGAGC TGGAAACAAG GCTGAGGGAG GTCAGCCTGG AGCACGAGGA GCAGAAGCTG 
GAGCTGAAGC GGCAGCTCAC GGAGCTGCAG CTGTCCCTGC AGGAGCGCGA GTCCCAGCTG 
ACGGCCCTGC AGGCCGCCCG GGCAGCGCTG GAGAGCCAGC TCCGCCAGGC GAAGACAGAG 
CTGGAGGAGA CAACCGCGGA AGCGGAGGAG GAGATCCAGG CGCTCACGGC ACATCGAGAT 
GAGATCCAGC GCAAATTCGA TGCCCTTCGC AACAGCTGCA CGGTCATCAC CGACCTGGAG 
GAGCAGCTGA ACCAGCTCAC CGAGGACAAC GCCGAGCTCA ACAACCAAAA CTTCTACCTG 
TCCAAACAAC TCGATGAGGC TTCCGGGGCC AATGACGAGA TTGTGCAGCT GCGGAGTGAG 
GTGGACCATC TCCGCCGTGA GATCACGGAG CGGGAGATGC AGCTCACCAG CCAGAAGCAA 
GCACAACTCA GTGCTCCTGA TTTGCAGACG ATGGAGGCTC TGAAGACGAC ATGCACCATG 
CTGGAGGAGC AGGTCCTGGA CCTGGAGGCC CTGAACGACG AGCTGCTGGA GAAGGAGCGC 
CAGTGGGAGG CCTGGCGGAG CGTCCTCGGC GACGAGAAGT CCCAGTTCGA GTGTCGAGTT 
CGAGAGCTAC AGAGGATGCT GGACACCGAG AAGCAGAGCA GGGCTAGGGC CGATCAGCGG 
ATCACCGAGT CGCGCCAGGT GGTGGAGTTG GCGGTGAAGG AACACAAGGC CGAGATTCTT 
GCTCTGCAGC AGGCTCTCAA GGAGCAGAAG CTCAAAGCCG AGAGCCTGTC GGACAAGCTC 
AACGACCTGG AGAAGAAGCA CGCCATGCTG GAGATGAACG CCCGGAGCTT ACAGCAGAAA 
CTAGAGACAG AGCGGGAGCT CAAACAGAGG CTTCTGGAGG AGCAAGCCAA ATTGCAGCAG 
CAGATGGACC TGCAGAAGAA CCACATCTTC AGACTGACGC AAGGGCTGCA GGAGGCGCTG 
GACCGGGCGG ATCTGCTGAA GACAGAAAGG AGCGACCTGG AGTACCAGCT GGAAAACATT 
CAGGTTCTCT ACTCTCACGA GAAAGTGAAA ATGGAAGGCA CAATCTCTCA GCAAACCAAA 
CTCATTGATT TCCTGCAAGC CAAAATGGAC CAGCCTGCTA AAAAGAAAAA GGGTTTATTT 
AGTCGACGGA AAGAGGACCC TGCTTTGCCC ACACAGGTTC CTCTGCAGTA CAATGAGCTG 
AAGCTAGCCC TGGAAAAGGA GAAAGCCCGA TGCGCGGAGC TGGAGGAGGC CCTTCAGAAG 
ACCCGCATCG AACTCCGCTC TGCGCGGGAG GAAGCTGCCC ACCGCAAAGC CACGGACCAC 
CCGCACCCGT CTACGCCAGC CACTGCGAGG CAGCAGATCG CCATGTCTGC CATTGTGCGG 
TCGCCCGAGC ACCAGCCCAG TGCCATGAGC CTGCTTGCCC CACCATCCAG CCGCAGAAAG 
GAGTCGTCAA CTCCAGAGGA ATTCAGCCGG CGTCTGAAAG AGCGCATGCA CCACAACATC 
CCTCACCGGT TTAATGTGGG CCTGAACATG AGAGCCACCA AGTGCGCCGT GTGTCTGGAT 
ACTGTGCACT TTGGACGCCA GGCATCCAAA TGCCTCGAAT GTCAAGTGAT GTGTCACCCC 
AAATGCTCCA CCTGCTTGCC CGCTACCTGT GGCCTGCCAG CTGAATATGC CACACACTTC 
ACTGAGGCCT TCTGCCGGGA CAAAATGAAC TCCCCGGGGC TCCAGAGCAA GGAGCCTGGC 
AGCAGCTTGC ACCTGGAAGG GTGGATGAAA GTGCCCAGAA ATAACAAACG GGGACAGCAA 
GGCTGGGACA GGAAGTACAT TGTTCTGGAG GGGTCAAAAG TCCTCATCTA TGACAATGAA 
GCCAGAGAAG CTGGACAGAG GCCGGTGGAA GAATTTGAGC TGTGCCTTCC TGACGGGGAC 
GTATCTATTC ATGGTGCCGT TGGTGCTTCA GAACTTGCAA ATACCGCCAA AGCAGATGTC 
CCATACATCC TGAAGATGGA GTCTCACCCA CACACCACCT GCTGGCCTGG GAGAACCCTC 
TACTTGCTAG CACCCAGCTT CCCCGACAAG CAGCGCTGGG TCACCGCCTT AGAATCTGTC 
GTCGCAGGTG GGAGAGTTTC TAGGGAAAAG GCCGAAGCCG ATGCTGCACG TGACCATACT 
TCGAGTGAGC ATCAGCCAGT GTGGGTTGAG AAATTACTTG GAAACTCTCT GCTGAAACTG 
GAGGGCGATG ACCGGCTTGA CATGAACTGC ACCCTGCCCT TCAGTGACCA GGTAGTGCTG 
GTGGGCACGG AGGAAGGCCT CTACGCCCTG AATGTCTTGA AAAACTCCTT AACCCACATC 
CCAGGGATTG GCGCAGTCTT CCAAATTTAC ATCATCAAGG ACCTGGAGAA GCTGCTCATG 
ATAGCAGGGG AAGAGCGGGC TCTGTGCCTG GTGGACGTGA AGAAGGTGAA GCAGTCCCTG 
GCGCAGTCAC ACCTGCCTGC CCAGCCCGAC GTCTCCCCCA ACATATTCGA AGCCGTCAAA 
GGCTGCCACT TGTTCGCTGC TGGCAAGATC GAGAACAGCC TGTGCATCTG CGCCGCTATG 
CCAAGCAAAG TCGTCATCCT CCGCTACAAT GACAACCTCA GCAAGTACTG CATCCGCAAG 
GAGATCGAGA CCTCAGAGCC CTGCAGCTGT ATCCACTTCA CCAACTACAG CATCCTCATT 
GGGACCAACA AATTCTATGA GATCGACATG AAGCAGTACA CGCTTGACGA GTTCCTGGAC 
AAGAACGACC ATTCCTTGGC ACCTGCTGTG TTCGCCTCCT CGTCCAACAG CTTCCCTGTC 
TCCATTGTGC AGGCGAACAG CGCCGGGCAG CGAGAAGAAT ACCTGCTGTG CTTCCACGAA 
TTTGGGGTGT TCGTGGATTC TTACGGAAGA CGTAGCCGCA CAGATGATCT CAAGTGGAGT 
CGCTTACCTC TGGCCTTCGC CTACAGAGAA CCTTATCTGT TTGTGACTCA CTTCAACTCC 
CTGGAAGTCA TTGAGATCCA GGCCAGATCC TCACTGGGGA GCCCTGCCCG AGCATATCTG 
GAAATTCCAA ACCCTCGCTA CCTGGGCCCC GCGATTTCCT CCGGAGCGAT TTACCTGGCC 
TCCTCATACC AGGACAAGTT AAGGGTCATA TGCTGCAAAG GAAACCTCGT GAAGGAGTCA 
GGCACTGAGC AGCACCGGGT GCCCTCCACC TCCCGCAGCC CCAACAAGCG AGGCCCACCA 
ACATACAACG AGCACATCAC CAAACGCGTG GCCTCCAGCC CGGCGCCACC GGAAGGCCCC 
AGCCACCCCC GAGAGCCAAG CACACCGCAC CGCTACCGAG ACAGAGAGGG TCGGACAGAG 
CTGCGCAGGG ACAAGTCTCC AGGCCGCCCT CTGGAGCGGG AGAAGTCCCC AGGCCGAATG 
CTCAGCACTA GGAGAGAGCG GTCCCCAGGG AGACTGTTTG AAGACAGCAG CAGGGGCCGG 
CTGCCTGCAG GAGCAGTGAG GACCCCACTG TCCCAGGTTA ACAAGGTCTG GGACCAGTCT 
TCAGTATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006530197.1
CDS	158..6526
Translation	MLKFKYGVRNPPEASASEPIASRASRLNLFFQGKPPLMTQQQMSALSREGMLDALFALFEECSQPALMKMKHVSSFVQKYSDTIAELRELQPSARDFEVRSLVGCGHFAEVQVVREKATGDVYAMKIMKKKALLAQEQVSFFEEERNILSRSTSPWIPQLQYAFQDKNNLYLVMEYQPGGDLLSLLNRYEDQLDESMIQFYLAELILAVHSVHQMGYVHRDIKPENILIDRTGHIKLVDFGSAAKMNSNKVDAKLPIGTPDYMAPEVLTVMNEDRRGTYGLDCDWWSVGVVAYEMVYGKTPFTEGTSARTFNNIMNFQRFLKFPDDPKVSSELLDLLQSLLCVQKERLKFEGLCCHPFFARTDWNNIRNSPPPFVPTLKSDDDTSNFDEPEKNSWVSSSVCQLSPSGFSGEELPFVGFSYSKALGYLGRSESVVSSLDSPAKVSSMEKKLLIKSKELQDSQDKCHKMEQEMTRLHRRVSEVEAVLSQKEVELKASETQRSLLEQDLATYITECSSLKRSLEQARMEVSQEDDKALQLLHDIREQSRKLQEIKEQEYQAQVEEMRLMMNQLEEDLVSARRRSDLYESELRESRLAAEEFKRKANECQHKLMKVVSHPPRGDSGGTALDDLHKMQGHAGLTSAKDQGKPEVGEYSKLEKINAEQQLKIQELQEKLEKAVKASTEATELLQNIRQAKERAERELEKLHNREDSSEGIKKKLVEAEERRHSLENKVKRLETMERRENRLKDDIQTKSEQIQQMADKILELEEKHREAQVSAQHLEVHLKQKEQHYEEKIKVLDNQIKKDLADKESLENMMQRHEEEAHEKGKILSEQKAMINAMDSKIRSLEQRIVELSEANKLAANSSLFTQRNMKAQEEMISELRQQKFYLETQAGKLEAQNRKLEEQLEKISHQDHSDKSRLLELETRLREVSLEHEEQKLELKRQLTELQLSLQERESQLTALQAARAALESQLRQAKTELEETTAEAEEEIQALTAHRDEIQRKFDALRNSCTVITDLEEQLNQLTEDNAELNNQNFYLSKQLDEASGANDEIVQLRSEVDHLRREITEREMQLTSQKQAQLSAPDLQTMEALKTTCTMLEEQVLDLEALNDELLEKERQWEAWRSVLGDEKSQFECRVRELQRMLDTEKQSRARADQRITESRQVVELAVKEHKAEILALQQALKEQKLKAESLSDKLNDLEKKHAMLEMNARSLQQKLETERELKQRLLEEQAKLQQQMDLQKNHIFRLTQGLQEALDRADLLKTERSDLEYQLENIQVLYSHEKVKMEGTISQQTKLIDFLQAKMDQPAKKKKGLFSRRKEDPALPTQVPLQYNELKLALEKEKARCAELEEALQKTRIELRSAREEAAHRKATDHPHPSTPATARQQIAMSAIVRSPEHQPSAMSLLAPPSSRRKESSTPEEFSRRLKERMHHNIPHRFNVGLNMRATKCAVCLDTVHFGRQASKCLECQVMCHPKCSTCLPATCGLPAEYATHFTEAFCRDKMNSPGLQSKEPGSSLHLEGWMKVPRNNKRGQQGWDRKYIVLEGSKVLIYDNEAREAGQRPVEEFELCLPDGDVSIHGAVGASELANTAKADVPYILKMESHPHTTCWPGRTLYLLAPSFPDKQRWVTALESVVAGGRVSREKAEADAARDHTSSEHQPVWVEKLLGNSLLKLEGDDRLDMNCTLPFSDQVVLVGTEEGLYALNVLKNSLTHIPGIGAVFQIYIIKDLEKLLMIAGEERALCLVDVKKVKQSLAQSHLPAQPDVSPNIFEAVKGCHLFAAGKIENSLCICAAMPSKVVILRYNDNLSKYCIRKEIETSEPCSCIHFTNYSILIGTNKFYEIDMKQYTLDEFLDKNDHSLAPAVFASSSNSFPVSIVQANSAGQREEYLLCFHEFGVFVDSYGRRSRTDDLKWSRLPLAFAYREPYLFVTHFNSLEVIEIQARSSLGSPARAYLEIPNPRYLGPAISSGAIYLASSYQDKLRVICCKGNLVKESGTEQHRVPSTSRSPNKRGPPTYNEHITKRVASSPAPPEGPSHPREPSTPHRYRDREGRTELRRDKSPGRPLEREKSPGRMLSTRRERSPGRLFEDSSRGRLPAGAVRTPLSQVNKVWDQSSV

Target ORF information:

RefSeq Version	XM_006530134.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus citron (Cit), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006530134.3

ORF Insert Sequence:

ATGTTGAAGT TCAAGTATGG TGTGCGGAAC CCGCCGGAGG CCAGTGCCTC CGAGCCCATT 
GCCAGTCGGG CCTCCAGGCT AAATCTCTTC TTCCAGGGGA AACCGCCCCT CATGACTCAA 
CAGCAGATGT CTGCTCTTTC CCGGGAAGGG ATGCTAGACG CCCTCTTCGC TCTCTTTGAA 
GAGTGCAGCC AACCCGCCCT GATGAAGATG AAGCACGTGA GCAGCTTTGT CCAGAAGTAT 
TCCGACACCA TAGCCGAGTT GCGGGAGCTG CAGCCGTCGG CGAGAGACTT CGAAGTTCGA 
AGCCTTGTGG GCTGTGGTCA CTTCGCTGAA GTGCAGGTGG TTAGAGAGAA GGCGACCGGG 
GACGTCTATG CCATGAAAAT CATGAAGAAG AAGGCTTTGC TGGCCCAGGA ACAGGTTTCA 
TTTTTCGAGG AGGAGAGGAA CATATTATCT CGGAGCACGA GTCCTTGGAT CCCCCAGTTA 
CAGTACGCCT TTCAGGACAA AAATAACCTT TACCTGGTCA TGGAATATCA GCCTGGAGGG 
GATTTGCTGT CGCTTCTGAA CAGATACGAG GACCAATTAG ATGAGAGCAT GATCCAGTTT 
TACCTTGCTG AGCTGATTTT GGCTGTCCAC AGCGTGCACC AGATGGGATA TGTGCATCGA 
GACATCAAGC CCGAGAACAT CCTCATCGAC CGGACGGGAC ACATCAAGCT GGTGGATTTT 
GGATCAGCCG CTAAGATGAA TTCAAATAAG GTGGATGCCA AACTCCCCAT TGGGACCCCG 
GATTACATGG CTCCGGAAGT GTTGACCGTG ATGAACGAGG ACCGAAGGGG CACATACGGC 
TTGGACTGTG ACTGGTGGTC TGTCGGAGTT GTTGCTTATG AGATGGTTTA TGGGAAGACC 
CCATTCACAG AGGGAACCTC CGCCCGGACC TTCAACAACA TCATGAACTT CCAGCGGTTT 
TTGAAGTTCC CAGATGACCC CAAAGTTAGC AGTGAGCTCC TTGATCTGCT TCAGAGTCTG 
CTGTGTGTCC AGAAAGAGAG ACTGAAGTTC GAGGGTCTCT GCTGCCACCC TTTCTTTGCC 
AGAACGGACT GGAACAACAT CCGTAACTCT CCTCCCCCCT TCGTCCCCAC CCTCAAGTCT 
GACGATGACA CCTCCAATTT TGATGAACCA GAGAAGAATT CGTGGGTTTC ATCCTCTGTG 
TGCCAGCTGA GCCCCTCGGG CTTCTCAGGC GAAGAGCTGC CGTTTGTGGG ATTTTCGTAC 
AGCAAGGCAC TGGGGTATCT TGGTAGATCT GAGTCTGTCG TGTCGAGTCT GGACTCCCCT 
GCCAAGGTTA GCTCCATGGA AAAGAAACTT CTCATCAAAA GCAAAGAGCT CCAAGACTCC 
CAGGACAAGT GTCACAAGAT GGAGCAGGAA ATGACCCGGT TACATCGCAG AGTGTCAGAG 
GTGGAGGCTG TGCTTAGTCA GAAGGAGGTG GAGCTGAAGG CCTCTGAGAC TCAGAGATCC 
CTCCTGGAGC AGGACCTTGC TACCTACATC ACAGAATGCA GTAGCTTAAA GCGAAGTTTG 
GAGCAAGCGC GGATGGAGGT GTCCCAGGAG GATGACAAAG CTCTGCAGCT TCTCCACGAC 
ATCCGAGAGC AGAGCCGGAA GCTCCAGGAG ATCAAGGAGC AGGAGTACCA GGCTCAGGTG 
GAGGAGATGA GGCTGATGAT GAATCAGCTG GAAGAAGACC TGGTGTCAGC CCGCAGACGC 
AGCGATCTCT ACGAGTCTGA GCTGAGGGAG TCTCGGCTTG CCGCCGAGGA ATTCAAGCGG 
AAGGCAAACG AATGTCAGCA CAAACTGATG AAGGTAGTCA GCCACCCTCC AAGAGGGGAC 
TCCGGGGGCA CGGCCCTGGA TGATCTTCAC AAGATGCAAG GCCACGCTGG CTTAACGAGT 
GCTAAGGACC AAGGGAAGCC TGAAGTGGGA GAATATTCCA AACTGGAGAA GATCAATGCT 
GAGCAGCAGC TGAAGATCCA GGAGCTCCAG GAGAAGCTGG AGAAGGCTGT AAAAGCCAGC 
ACAGAGGCCA CCGAGCTCCT GCAGAATATC CGCCAGGCAA AGGAGCGAGC AGAGCGGGAG 
CTAGAGAAGC TACACAACCG GGAAGACTCC TCCGAGGGCA TCAAAAAGAA GCTGGTGGAA 
GCCGAGGAAC GCCGCCACTC CCTGGAGAAC AAGGTAAAGA GACTAGAGAC CATGGAGCGT 
AGAGAGAACA GACTGAAGGA TGACATCCAG ACAAAGTCCG AACAGATCCA GCAGATGGCT 
GATAAAATTC TGGAGCTGGA GGAGAAACAT CGGGAGGCTC AGGTCTCAGC TCAACATCTA 
GAAGTACACT TGAAACAGAA AGAACAGCAC TACGAGGAAA AGATCAAGGT ATTGGACAAT 
CAGATAAAGA AGGACCTGGC CGACAAGGAG AGCCTGGAGA ACATGATGCA GAGACACGAG 
GAGGAGGCCC ACGAGAAGGG CAAGATCCTC AGCGAGCAGA AGGCGATGAT CAACGCGATG 
GATTCCAAGA TCCGATCCCT GGAGCAGAGG ATCGTGGAGC TGTCGGAAGC CAACAAGCTT 
GCGGCAAACA GCAGTCTCTT CACCCAGAGG AACATGAAGG CCCAGGAAGA GATGATCTCA 
GAACTCAGGC AGCAGAAATT TTACCTGGAG ACGCAGGCCG GAAAGCTGGA GGCCCAGAAC 
CGAAAGCTGG AAGAGCAACT GGAGAAGATC AGCCACCAAG ATCACAGTGA CAAGAGTCGG 
CTGCTGGAGC TGGAAACAAG GCTGAGGGAG GTCAGCCTGG AGCACGAGGA GCAGAAGCTG 
GAGCTGAAGC GGCAGCTCAC GGAGCTGCAG CTGTCCCTGC AGGAGCGCGA GTCCCAGCTG 
ACGGCCCTGC AGGCCGCCCG GGCAGCGCTG GAGAGCCAGC TCCGCCAGGC GAAGACAGAG 
CTGGAGGAGA CAACCGCGGA AGCGGAGGAG GAGATCCAGG CGCTCACGGC ACATCGAGAT 
GAGATCCAGC GCAAATTCGA TGCCCTTCGC AACAGCTGCA CGGTCATCAC CGACCTGGAG 
GAGCAGCTGA ACCAGCTCAC CGAGGACAAC GCCGAGCTCA ACAACCAAAA CTTCTACCTG 
TCCAAACAAC TCGATGAGGC TTCCGGGGCC AATGACGAGA TTGTGCAGCT GCGGAGTGAG 
GTGGACCATC TCCGCCGTGA GATCACGGAG CGGGAGATGC AGCTCACCAG CCAGAAGCAA 
GCACAACTCA GTGCTCCTGA TTTGCAGACG ATGGAGGCTC TGAAGACGAC ATGCACCATG 
CTGGAGGAGC AGGTCCTGGA CCTGGAGGCC CTGAACGACG AGCTGCTGGA GAAGGAGCGC 
CAGTGGGAGG CCTGGCGGAG CGTCCTCGGC GACGAGAAGT CCCAGTTCGA GTGTCGAGTT 
CGAGAGCTAC AGAGGATGCT GGACACCGAG AAGCAGAGCA GGGCTAGGGC CGATCAGCGG 
ATCACCGAGT CGCGCCAGGT GGTGGAGTTG GCGGTGAAGG AACACAAGGC CGAGATTCTT 
GCTCTGCAGC AGGCTCTCAA GGAGCAGAAG CTCAAAGCCG AGAGCCTGTC GGACAAGCTC 
AACGACCTGG AGAAGAAGCA CGCCATGCTG GAGATGAACG CCCGGAGCTT ACAGCAGAAA 
CTAGAGACAG AGCGGGAGCT CAAACAGAGG CTTCTGGAGG AGCAAGCCAA ATTGCAGCAG 
CAGATGGACC TGCAGAAGAA CCACATCTTC AGACTGACGC AAGGGCTGCA GGAGGCGCTG 
GACCGGGCGG ATCTGCTGAA GACAGAAAGG AGCGACCTGG AGTACCAGCT GGAAAACATT 
CAGGTTCTCT ACTCTCACGA GAAAGTGAAA ATGGAAGGCA CAATCTCTCA GCAAACCAAA 
CTCATTGATT TCCTGCAAGC CAAAATGGAC CAGCCTGCTA AAAAGAAAAA GGGTTTATTT 
AGTCGACGGA AAGAGGACCC TGCTTTGCCC ACACAGGTTC CTCTGCAGTA CAATGAGCTG 
AAGCTAGCCC TGGAAAAGGA GAAAGCCCGA TGCGCGGAGC TGGAGGAGGC CCTTCAGAAG 
ACCCGCATCG AACTCCGCTC TGCGCGGGAG GAAGCTGCCC ACCGCAAAGC CACGGACCAC 
CCGCACCCGT CTACGCCAGC CACTGCGAGG CAGCAGATCG CCATGTCTGC CATTGTGCGG 
TCGCCCGAGC ACCAGCCCAG TGCCATGAGC CTGCTTGCCC CACCATCCAG CCGCAGAAAG 
GAGTCGTCAA CTCCAGAGGA ATTCAGCCGG CGTCTGAAAG AGCGCATGCA CCACAACATC 
CCTCACCGGT TTAATGTGGG CCTGAACATG AGAGCCACCA AGTGCGCCGT GTGTCTGGAT 
ACTGTGCACT TTGGACGCCA GGCATCCAAA TGCCTCGAAT GTCAAGTGAT GTGTCACCCC 
AAATGCTCCA CCTGCTTGCC CGCTACCTGT GGCCTGCCAG CTGAATATGC CACACACTTC 
ACTGAGGCCT TCTGCCGGGA CAAAATGAAC TCCCCGGGGC TCCAGAGCAA GGAGCCTGGC 
AGCAGCTTGC ACCTGGAAGG GTGGATGAAA GTGCCCAGAA ATAACAAACG GGGACAGCAA 
GGCTGGGACA GGAAGTACAT TGTTCTGGAG GGGTCAAAAG TCCTCATCTA TGACAATGAA 
GCCAGAGAAG CTGGACAGAG GCCGGTGGAA GAATTTGAGC TGTGCCTTCC TGACGGGGAC 
GTATCTATTC ATGGTGCCGT TGGTGCTTCA GAACTTGCAA ATACCGCCAA AGCAGATGTC 
CCATACATCC TGAAGATGGA GTCTCACCCA CACACCACCT GCTGGCCTGG GAGAACCCTC 
TACTTGCTAG CACCCAGCTT CCCCGACAAG CAGCGCTGGG TCACCGCCTT AGAATCTGTC 
GTCGCAGGTG GGAGAGTTTC TAGGGAAAAG GCCGAAGCCG ATGCTGCACG TGACCATACT 
TCGAGTGAGC ATCAGCCAGT GTGGGTTGAG AAATTACTTG GAAACTCTCT GCTGAAACTG 
GAGGGCGATG ACCGGCTTGA CATGAACTGC ACCCTGCCCT TCAGTGACCA GGTAGTGCTG 
GTGGGCACGG AGGAAGGCCT CTACGCCCTG AATGTCTTGA AAAACTCCTT AACCCACATC 
CCAGGGATTG GCGCAGTCTT CCAAATTTAC ATCATCAAGG ACCTGGAGAA GCTGCTCATG 
ATAGCAGGGG AAGAGCGGGC TCTGTGCCTG GTGGACGTGA AGAAGGTGAA GCAGTCCCTG 
GCGCAGTCAC ACCTGCCTGC CCAGCCCGAC GTCTCCCCCA ACATATTCGA AGCCGTCAAA 
GGCTGCCACT TGTTCGCTGC TGGCAAGATC GAGAACAGCC TGTGCATCTG CGCCGCTATG 
CCAAGCAAAG TCGTCATCCT CCGCTACAAT GACAACCTCA GCAAGTACTG CATCCGCAAG 
GAGATCGAGA CCTCAGAGCC CTGCAGCTGT ATCCACTTCA CCAACTACAG CATCCTCATT 
GGGACCAACA AATTCTATGA GATCGACATG AAGCAGTACA CGCTTGACGA GTTCCTGGAC 
AAGAACGACC ATTCCTTGGC ACCTGCTGTG TTCGCCTCCT CGTCCAACAG CTTCCCTGTC 
TCCATTGTGC AGGCGAACAG CGCCGGGCAG CGAGAAGAAT ACCTGCTGTG CTTCCACGAA 
TTTGGGGTGT TCGTGGATTC TTACGGAAGA CGTAGCCGCA CAGATGATCT CAAGTGGAGT 
CGCTTACCTC TGGCCTTCGC CTACAGAGAA CCTTATCTGT TTGTGACTCA CTTCAACTCC 
CTGGAAGTCA TTGAGATCCA GGCCAGATCC TCACTGGGGA GCCCTGCCCG AGCATATCTG 
GAAATTCCAA ACCCTCGCTA CCTGGGCCCC GCGATTTCCT CCGGAGCGAT TTACCTGGCC 
TCCTCATACC AGGACAAGTT AAGGGTCATA TGCTGCAAAG GAAACCTCGT GAAGGAGTCA 
GGCACTGAGC AGCACCGGGT GCCCTCCACC TCCCGCAGCC CCAACAAGCG AGGCCCACCA 
ACATACAACG AGCACATCAC CAAACGCGTG GCCTCCAGCC CGGCGCCACC GGAAGGCCCC 
AGCCACCCCC GAGAGCCAAG CACACCGCAC CGCTACCGAG ACAGAGAGGG TCGGACAGAG 
CTGCGCAGGG ACAAGTCTCC AGGCCGCCCT CTGGAGCGGG AGAAGTCCCC AGGCCGAATG 
CTCAGCACTA GGAGAGAGCG GTCCCCAGGG AGACTGTTTG AAGACAGCAG CAGGGGCCGG 
CTGCCTGCAG GAGCAGTGAG GACCCCACTG TCCCAGGTTA ACAAGGTCTG GGACCAGTCT 
TCAGTATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cit ( XM_006530135.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006530198.1 Mus musculus citron (Cit), transcript variant X6, mRNA.

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CloneID	OMu35776
Clone ID Related Accession (Same CDS sequence)	XM_006530135.4 , XM_006530135.3
Accession Version	XM_006530135.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6345bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	citron Rho-interacting kinase isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000071.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006530135.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTGAAGT TCAAGTATGG TGTGCGGAAC CCGCCGGAGG CCAGTGCCTC CGAGCCCATT 
GCCAGTCGGG CCTCCAGGCT AAATCTCTTC TTCCAGGGGA AACCGCCCCT CATGACTCAA 
CAGCAGATGT CTGCTCTTTC CCGGGAAGGG ATGCTAGACG CCCTCTTCGC TCTCTTTGAA 
GAGTGCAGCC AACCCGCCCT GATGAAGATG AAGCACGTGA GCAGCTTTGT CCAGAAGTAT 
TCCGACACCA TAGCCGAGTT GCGGGAGCTG CAGCCGTCGG CGAGAGACTT CGAAGTTCGA 
AGCCTTGTGG GCTGTGGTCA CTTCGCTGAA GTGCAGGTGG TTAGAGAGAA GGCGACCGGG 
GACGTCTATG CCATGAAAAT CATGAAGAAG AAGGCTTTGC TGGCCCAGGA ACAGGTTTCA 
TTTTTCGAGG AGGAGAGGAA CATATTATCT CGGAGCACGA GTCCTTGGAT CCCCCAGTTA 
CAGTACGCCT TTCAGGACAA AAATAACCTT TACCTGGTCA TGGAATATCA GCCTGGAGGG 
GATTTGCTGT CGCTTCTGAA CAGATACGAG GACCAATTAG ATGAGAGCAT GATCCAGTTT 
TACCTTGCTG AGCTGATTTT GGCTGTCCAC AGCGTGCACC AGATGGGATA TGTGCATCGA 
GACATCAAGC CCGAGAACAT CCTCATCGAC CGGACGGGAC ACATCAAGCT GGTGGATTTT 
GGATCAGCCG CTAAGATGAA TTCAAATAAG GTGGATGCCA AACTCCCCAT TGGGACCCCG 
GATTACATGG CTCCGGAAGT GTTGACCGTG ATGAACGAGG ACCGAAGGGG CACATACGGC 
TTGGACTGTG ACTGGTGGTC TGTCGGAGTT GTTGCTTATG AGATGGTTTA TGGGAAGACC 
CCATTCACAG AGGGAACCTC CGCCCGGACC TTCAACAACA TCATGAACTT CCAGCGGTTT 
TTGAAGTTCC CAGATGACCC CAAAGTTAGC AGTGAGCTCC TTGATCTGCT TCAGAGTCTG 
CTGTGTGTCC AGAAAGAGAG ACTGAAGTTC GAGGGTCTCT GCTGCCACCC TTTCTTTGCC 
AGAACGGACT GGAACAACAT CCGTAACTCT CCTCCCCCCT TCGTCCCCAC CCTCAAGTCT 
GACGATGACA CCTCCAATTT TGATGAACCA GAGAAGAATT CGTGGGTTTC ATCCTCTGTG 
TGCCAGCTGA GCCCCTCGGG CTTCTCAGGC GAAGAGCTGC CGTTTGTGGG ATTTTCGTAC 
AGCAAGGCAC TGGGGTATCT TGGTAGATCT GAGTCTGTCG TGTCGAGTCT GGACTCCCCT 
GCCAAGGTTA GCTCCATGGA AAAGAAACTT CTCATCAAAA GCAAAGAGCT CCAAGACTCC 
CAGGACAAGT GTCACAAGAT GGAGCAGGAA ATGACCCGGT TACATCGCAG AGTGTCAGAG 
GTGGAGGCTG TGCTTAGTCA GAAGGAGGTG GAGCTGAAGG CCTCTGAGAC TCAGAGATCC 
CTCCTGGAGC AGGACCTTGC TACCTACATC ACAGAATGCA GTAGCTTAAA GCGAAGTTTG 
GAGCAAGCGC GGATGGAGGT GTCCCAGGAG GATGACAAAG CTCTGCAGCT TCTCCACGAC 
ATCCGAGAGC AGAGCCGGAA GCTCCAGGAG ATCAAGGAGC AGGAGTACCA GGCTCAGGTG 
GAGGAGATGA GGCTGATGAT GAATCAGCTG GAAGAAGACC TGGTGTCAGC CCGCAGACGC 
AGCGATCTCT ACGAGTCTGA GCTGAGGGAG TCTCGGCTTG CCGCCGAGGA ATTCAAGCGG 
AAGGCAAACG AATGTCAGCA CAAACTGATG AAGGTAGTCA GCCACCCTCC AAGAGGGGAC 
TCCGGGGGCA CGGCCCTGGA TGATCTTCAC AAGATGCAAG GCCACGCTGG CTTAACGAGT 
GCTAAGGACC AAGGGAAGCC TGAAGTGGGA GAATATTCCA AACTGGAGAA GATCAATGCT 
GAGCAGCAGC TGAAGATCCA GGAGCTCCAG GAGAAGCTGG AGAAGGCTGT AAAAGCCAGC 
ACAGAGGCCA CCGAGCTCCT GCAGAATATC CGCCAGGCAA AGGAGCGAGC AGAGCGGGAG 
CTAGAGAAGC TACACAACCG GGAAGACTCC TCCGAGGGCA TCAAAAAGAA GCTGGTGGAA 
GCCGAGGAAC GCCGCCACTC CCTGGAGAAC AAGGTAAAGA GACTAGAGAC CATGGAGCGT 
AGAGAGAACA GACTGAAGGA TGACATCCAG ACAAAGTCCG AACAGATCCA GCAGATGGCT 
GATAAAATTC TGGAGCTGGA GGAGAAACAT CGGGAGGCTC AGGTCTCAGC TCAACATCTA 
GAAGTACACT TGAAACAGAA AGAACAGCAC TACGAGGAAA AGATCAAGGT ATTGGACAAT 
CAGATAAAGA AGGACCTGGC CGACAAGGAG AGCCTGGAGA ACATGATGCA GAGACACGAG 
GAGGAGGCCC ACGAGAAGGG CAAGATCCTC AGCGAGCAGA AGGCGATGAT CAACGCGATG 
GATTCCAAGA TCCGATCCCT GGAGCAGAGG ATCGTGGAGC TGTCGGAAGC CAACAAGCTT 
GCGGCAAACA GCAGTCTCTT CACCCAGAGG AACATGAAGG CCCAGGAAGA GATGATCTCA 
GAACTCAGGC AGCAGAAATT TTACCTGGAG ACGCAGGCCG GAAAGCTGGA GGCCCAGAAC 
CGAAAGCTGG AAGAGCAACT GGAGAAGATC AGCCACCAAG ATCACAGTGA CAAGAGTCGG 
CTGCTGGAGC TGGAAACAAG GCTGAGGGAG GTCAGCCTGG AGCACGAGGA GCAGAAGCTG 
GAGCTGAAGC GGCAGCTCAC GGAGCTGCAG CTGTCCCTGC AGGAGCGCGA GTCCCAGCTG 
ACGGCCCTGC AGGCCGCCCG GGCAGCGCTG GAGAGCCAGC TCCGCCAGGC GAAGACAGAG 
CTGGAGGAGA CAACCGCGGA AGCGGAGGAG GAGATCCAGG CGCTCACGGC ACATCGAGAT 
GAGATCCAGC GCAAATTCGA TGCCCTTCGC AACAGCTGCA CGGTCATCAC CGACCTGGAG 
GAGCAGCTGA ACCAGCTCAC CGAGGACAAC GCCGAGCTCA ACAACCAAAA CTTCTACCTG 
TCCAAACAAC TCGATGAGGC TTCCGGGGCC AATGACGAGA TTGTGCAGCT GCGGAGTGAG 
GTGGACCATC TCCGCCGTGA GATCACGGAG CGGGAGATGC AGCTCACCAG CCAGAAGCAA 
ACGATGGAGG CTCTGAAGAC GACATGCACC ATGCTGGAGG AGCAGGTCCT GGACCTGGAG 
GCCCTGAACG ACGAGCTGCT GGAGAAGGAG CGCCAGTGGG AGGCCTGGCG GAGCGTCCTC 
GGCGACGAGA AGTCCCAGTT CGAGTGTCGA GTTCGAGAGC TACAGAGGAT GCTGGACACC 
GAGAAGCAGA GCAGGGCTAG GGCCGATCAG CGGATCACCG AGTCGCGCCA GGTGGTGGAG 
TTGGCGGTGA AGGAACACAA GGCCGAGATT CTTGCTCTGC AGCAGGCTCT CAAGGAGCAG 
AAGCTCAAAG CCGAGAGCCT GTCGGACAAG CTCAACGACC TGGAGAAGAA GCACGCCATG 
CTGGAGATGA ACGCCCGGAG CTTACAGCAG AAACTAGAGA CAGAGCGGGA GCTCAAACAG 
AGGCTTCTGG AGGAGCAAGC CAAATTGCAG CAGCAGATGG ACCTGCAGAA GAACCACATC 
TTCAGACTGA CGCAAGGGCT GCAGGAGGCG CTGGACCGGG CGGATCTGCT GAAGACAGAA 
AGGAGCGACC TGGAGTACCA GCTGGAAAAC ATTCAGGTTC TCTACTCTCA CGAGAAAGTG 
AAAATGGAAG GCACAATCTC TCAGCAAACC AAACTCATTG ATTTCCTGCA AGCCAAAATG 
GACCAGCCTG CTAAAAAGAA AAAGGGTTTA TTTAGTCGAC GGAAAGAGGA CCCTGCTTTG 
CCCACACAGG TTCCTCTGCA GTACAATGAG CTGAAGCTAG CCCTGGAAAA GGAGAAAGCC 
CGATGCGCGG AGCTGGAGGA GGCCCTTCAG AAGACCCGCA TCGAACTCCG CTCTGCGCGG 
GAGGAAGCTG CCCACCGCAA AGCCACGGAC CACCCGCACC CGTCTACGCC AGCCACTGCG 
AGGCAGCAGA TCGCCATGTC TGCCATTGTG CGGTCGCCCG AGCACCAGCC CAGTGCCATG 
AGCCTGCTTG CCCCACCATC CAGCCGCAGA AAGGAGTCGT CAACTCCAGA GGAATTCAGC 
CGGCGTCTGA AAGAGCGCAT GCACCACAAC ATCCCTCACC GGTTTAATGT GGGCCTGAAC 
ATGAGAGCCA CCAAGTGCGC CGTGTGTCTG GATACTGTGC ACTTTGGACG CCAGGCATCC 
AAATGCCTCG AATGTCAAGT GATGTGTCAC CCCAAATGCT CCACCTGCTT GCCCGCTACC 
TGTGGCCTGC CAGCTGAATA TGCCACACAC TTCACTGAGG CCTTCTGCCG GGACAAAATG 
AACTCCCCGG GGCTCCAGAG CAAGGAGCCT GGCAGCAGCT TGCACCTGGA AGGGTGGATG 
AAAGTGCCCA GAAATAACAA ACGGGGACAG CAAGGCTGGG ACAGGAAGTA CATTGTTCTG 
GAGGGGTCAA AAGTCCTCAT CTATGACAAT GAAGCCAGAG AAGCTGGACA GAGGCCGGTG 
GAAGAATTTG AGCTGTGCCT TCCTGACGGG GACGTATCTA TTCATGGTGC CGTTGGTGCT 
TCAGAACTTG CAAATACCGC CAAAGCAGAT GTCCCATACA TCCTGAAGAT GGAGTCTCAC 
CCACACACCA CCTGCTGGCC TGGGAGAACC CTCTACTTGC TAGCACCCAG CTTCCCCGAC 
AAGCAGCGCT GGGTCACCGC CTTAGAATCT GTCGTCGCAG GTGGGAGAGT TTCTAGGGAA 
AAGGCCGAAG CCGATGCTGC ACGTGACCAT ACTTCGAGTG AGCATCAGCC AGTGTGGGTT 
GAGAAATTAC TTGGAAACTC TCTGCTGAAA CTGGAGGGCG ATGACCGGCT TGACATGAAC 
TGCACCCTGC CCTTCAGTGA CCAGGTAGTG CTGGTGGGCA CGGAGGAAGG CCTCTACGCC 
CTGAATGTCT TGAAAAACTC CTTAACCCAC ATCCCAGGGA TTGGCGCAGT CTTCCAAATT 
TACATCATCA AGGACCTGGA GAAGCTGCTC ATGATAGCAG GGGAAGAGCG GGCTCTGTGC 
CTGGTGGACG TGAAGAAGGT GAAGCAGTCC CTGGCGCAGT CACACCTGCC TGCCCAGCCC 
GACGTCTCCC CCAACATATT CGAAGCCGTC AAAGGCTGCC ACTTGTTCGC TGCTGGCAAG 
ATCGAGAACA GCCTGTGCAT CTGCGCCGCT ATGCCAAGCA AAGTCGTCAT CCTCCGCTAC 
AATGACAACC TCAGCAAGTA CTGCATCCGC AAGGAGATCG AGACCTCAGA GCCCTGCAGC 
TGTATCCACT TCACCAACTA CAGCATCCTC ATTGGGACCA ACAAATTCTA TGAGATCGAC 
ATGAAGCAGT ACACGCTTGA CGAGTTCCTG GACAAGAACG ACCATTCCTT GGCACCTGCT 
GTGTTCGCCT CCTCGTCCAA CAGCTTCCCT GTCTCCATTG TGCAGGCGAA CAGCGCCGGG 
CAGCGAGAAG AATACCTGCT GTGCTTCCAC GAATTTGGGG TGTTCGTGGA TTCTTACGGA 
AGACGTAGCC GCACAGATGA TCTCAAGTGG AGTCGCTTAC CTCTGGCCTT CGCCTACAGA 
GAACCTTATC TGTTTGTGAC TCACTTCAAC TCCCTGGAAG TCATTGAGAT CCAGGCCAGA 
TCCTCACTGG GGAGCCCTGC CCGAGCATAT CTGGAAATTC CAAACCCTCG CTACCTGGGC 
CCCGCGATTT CCTCCGGAGC GATTTACCTG GCCTCCTCAT ACCAGGACAA GTTAAGGGTC 
ATATGCTGCA AAGGAAACCT CGTGAAGGAG TCAGGCACTG AGCAGCACCG GGTGCCCTCC 
ACCTCCCGCA GCAGCCCCAA CAAGCGAGGC CCACCAACAT ACAACGAGCA CATCACCAAA 
CGCGTGGCCT CCAGCCCGGC GCCACCGGAA GGCCCCAGCC ACCCCCGAGA GCCAAGCACA 
CCGCACCGCT ACCGAGACAG AGAGGGTCGG ACAGAGCTGC GCAGGGACAA GTCTCCAGGC 
CGCCCTCTGG AGCGGGAGAA GTCCCCAGGC CGAATGCTCA GCACTAGGAG AGAGCGGTCC 
CCAGGGAGAC TGTTTGAAGA CAGCAGCAGG GGCCGGCTGC CTGCAGGAGC AGTGAGGACC 
CCACTGTCCC AGGTTAACAA GGTCTGGGAC CAGTCTTCAG TATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006530198.1
CDS	158..6502
Translation	MLKFKYGVRNPPEASASEPIASRASRLNLFFQGKPPLMTQQQMSALSREGMLDALFALFEECSQPALMKMKHVSSFVQKYSDTIAELRELQPSARDFEVRSLVGCGHFAEVQVVREKATGDVYAMKIMKKKALLAQEQVSFFEEERNILSRSTSPWIPQLQYAFQDKNNLYLVMEYQPGGDLLSLLNRYEDQLDESMIQFYLAELILAVHSVHQMGYVHRDIKPENILIDRTGHIKLVDFGSAAKMNSNKVDAKLPIGTPDYMAPEVLTVMNEDRRGTYGLDCDWWSVGVVAYEMVYGKTPFTEGTSARTFNNIMNFQRFLKFPDDPKVSSELLDLLQSLLCVQKERLKFEGLCCHPFFARTDWNNIRNSPPPFVPTLKSDDDTSNFDEPEKNSWVSSSVCQLSPSGFSGEELPFVGFSYSKALGYLGRSESVVSSLDSPAKVSSMEKKLLIKSKELQDSQDKCHKMEQEMTRLHRRVSEVEAVLSQKEVELKASETQRSLLEQDLATYITECSSLKRSLEQARMEVSQEDDKALQLLHDIREQSRKLQEIKEQEYQAQVEEMRLMMNQLEEDLVSARRRSDLYESELRESRLAAEEFKRKANECQHKLMKVVSHPPRGDSGGTALDDLHKMQGHAGLTSAKDQGKPEVGEYSKLEKINAEQQLKIQELQEKLEKAVKASTEATELLQNIRQAKERAERELEKLHNREDSSEGIKKKLVEAEERRHSLENKVKRLETMERRENRLKDDIQTKSEQIQQMADKILELEEKHREAQVSAQHLEVHLKQKEQHYEEKIKVLDNQIKKDLADKESLENMMQRHEEEAHEKGKILSEQKAMINAMDSKIRSLEQRIVELSEANKLAANSSLFTQRNMKAQEEMISELRQQKFYLETQAGKLEAQNRKLEEQLEKISHQDHSDKSRLLELETRLREVSLEHEEQKLELKRQLTELQLSLQERESQLTALQAARAALESQLRQAKTELEETTAEAEEEIQALTAHRDEIQRKFDALRNSCTVITDLEEQLNQLTEDNAELNNQNFYLSKQLDEASGANDEIVQLRSEVDHLRREITEREMQLTSQKQTMEALKTTCTMLEEQVLDLEALNDELLEKERQWEAWRSVLGDEKSQFECRVRELQRMLDTEKQSRARADQRITESRQVVELAVKEHKAEILALQQALKEQKLKAESLSDKLNDLEKKHAMLEMNARSLQQKLETERELKQRLLEEQAKLQQQMDLQKNHIFRLTQGLQEALDRADLLKTERSDLEYQLENIQVLYSHEKVKMEGTISQQTKLIDFLQAKMDQPAKKKKGLFSRRKEDPALPTQVPLQYNELKLALEKEKARCAELEEALQKTRIELRSAREEAAHRKATDHPHPSTPATARQQIAMSAIVRSPEHQPSAMSLLAPPSSRRKESSTPEEFSRRLKERMHHNIPHRFNVGLNMRATKCAVCLDTVHFGRQASKCLECQVMCHPKCSTCLPATCGLPAEYATHFTEAFCRDKMNSPGLQSKEPGSSLHLEGWMKVPRNNKRGQQGWDRKYIVLEGSKVLIYDNEAREAGQRPVEEFELCLPDGDVSIHGAVGASELANTAKADVPYILKMESHPHTTCWPGRTLYLLAPSFPDKQRWVTALESVVAGGRVSREKAEADAARDHTSSEHQPVWVEKLLGNSLLKLEGDDRLDMNCTLPFSDQVVLVGTEEGLYALNVLKNSLTHIPGIGAVFQIYIIKDLEKLLMIAGEERALCLVDVKKVKQSLAQSHLPAQPDVSPNIFEAVKGCHLFAAGKIENSLCICAAMPSKVVILRYNDNLSKYCIRKEIETSEPCSCIHFTNYSILIGTNKFYEIDMKQYTLDEFLDKNDHSLAPAVFASSSNSFPVSIVQANSAGQREEYLLCFHEFGVFVDSYGRRSRTDDLKWSRLPLAFAYREPYLFVTHFNSLEVIEIQARSSLGSPARAYLEIPNPRYLGPAISSGAIYLASSYQDKLRVICCKGNLVKESGTEQHRVPSTSRSSPNKRGPPTYNEHITKRVASSPAPPEGPSHPREPSTPHRYRDREGRTELRRDKSPGRPLEREKSPGRMLSTRRERSPGRLFEDSSRGRLPAGAVRTPLSQVNKVWDQSSV

Target ORF information:

RefSeq Version	XM_006530135.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus citron (Cit), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006530135.3

ORF Insert Sequence:

ATGTTGAAGT TCAAGTATGG TGTGCGGAAC CCGCCGGAGG CCAGTGCCTC CGAGCCCATT 
GCCAGTCGGG CCTCCAGGCT AAATCTCTTC TTCCAGGGGA AACCGCCCCT CATGACTCAA 
CAGCAGATGT CTGCTCTTTC CCGGGAAGGG ATGCTAGACG CCCTCTTCGC TCTCTTTGAA 
GAGTGCAGCC AACCCGCCCT GATGAAGATG AAGCACGTGA GCAGCTTTGT CCAGAAGTAT 
TCCGACACCA TAGCCGAGTT GCGGGAGCTG CAGCCGTCGG CGAGAGACTT CGAAGTTCGA 
AGCCTTGTGG GCTGTGGTCA CTTCGCTGAA GTGCAGGTGG TTAGAGAGAA GGCGACCGGG 
GACGTCTATG CCATGAAAAT CATGAAGAAG AAGGCTTTGC TGGCCCAGGA ACAGGTTTCA 
TTTTTCGAGG AGGAGAGGAA CATATTATCT CGGAGCACGA GTCCTTGGAT CCCCCAGTTA 
CAGTACGCCT TTCAGGACAA AAATAACCTT TACCTGGTCA TGGAATATCA GCCTGGAGGG 
GATTTGCTGT CGCTTCTGAA CAGATACGAG GACCAATTAG ATGAGAGCAT GATCCAGTTT 
TACCTTGCTG AGCTGATTTT GGCTGTCCAC AGCGTGCACC AGATGGGATA TGTGCATCGA 
GACATCAAGC CCGAGAACAT CCTCATCGAC CGGACGGGAC ACATCAAGCT GGTGGATTTT 
GGATCAGCCG CTAAGATGAA TTCAAATAAG GTGGATGCCA AACTCCCCAT TGGGACCCCG 
GATTACATGG CTCCGGAAGT GTTGACCGTG ATGAACGAGG ACCGAAGGGG CACATACGGC 
TTGGACTGTG ACTGGTGGTC TGTCGGAGTT GTTGCTTATG AGATGGTTTA TGGGAAGACC 
CCATTCACAG AGGGAACCTC CGCCCGGACC TTCAACAACA TCATGAACTT CCAGCGGTTT 
TTGAAGTTCC CAGATGACCC CAAAGTTAGC AGTGAGCTCC TTGATCTGCT TCAGAGTCTG 
CTGTGTGTCC AGAAAGAGAG ACTGAAGTTC GAGGGTCTCT GCTGCCACCC TTTCTTTGCC 
AGAACGGACT GGAACAACAT CCGTAACTCT CCTCCCCCCT TCGTCCCCAC CCTCAAGTCT 
GACGATGACA CCTCCAATTT TGATGAACCA GAGAAGAATT CGTGGGTTTC ATCCTCTGTG 
TGCCAGCTGA GCCCCTCGGG CTTCTCAGGC GAAGAGCTGC CGTTTGTGGG ATTTTCGTAC 
AGCAAGGCAC TGGGGTATCT TGGTAGATCT GAGTCTGTCG TGTCGAGTCT GGACTCCCCT 
GCCAAGGTTA GCTCCATGGA AAAGAAACTT CTCATCAAAA GCAAAGAGCT CCAAGACTCC 
CAGGACAAGT GTCACAAGAT GGAGCAGGAA ATGACCCGGT TACATCGCAG AGTGTCAGAG 
GTGGAGGCTG TGCTTAGTCA GAAGGAGGTG GAGCTGAAGG CCTCTGAGAC TCAGAGATCC 
CTCCTGGAGC AGGACCTTGC TACCTACATC ACAGAATGCA GTAGCTTAAA GCGAAGTTTG 
GAGCAAGCGC GGATGGAGGT GTCCCAGGAG GATGACAAAG CTCTGCAGCT TCTCCACGAC 
ATCCGAGAGC AGAGCCGGAA GCTCCAGGAG ATCAAGGAGC AGGAGTACCA GGCTCAGGTG 
GAGGAGATGA GGCTGATGAT GAATCAGCTG GAAGAAGACC TGGTGTCAGC CCGCAGACGC 
AGCGATCTCT ACGAGTCTGA GCTGAGGGAG TCTCGGCTTG CCGCCGAGGA ATTCAAGCGG 
AAGGCAAACG AATGTCAGCA CAAACTGATG AAGGTAGTCA GCCACCCTCC AAGAGGGGAC 
TCCGGGGGCA CGGCCCTGGA TGATCTTCAC AAGATGCAAG GCCACGCTGG CTTAACGAGT 
GCTAAGGACC AAGGGAAGCC TGAAGTGGGA GAATATTCCA AACTGGAGAA GATCAATGCT 
GAGCAGCAGC TGAAGATCCA GGAGCTCCAG GAGAAGCTGG AGAAGGCTGT AAAAGCCAGC 
ACAGAGGCCA CCGAGCTCCT GCAGAATATC CGCCAGGCAA AGGAGCGAGC AGAGCGGGAG 
CTAGAGAAGC TACACAACCG GGAAGACTCC TCCGAGGGCA TCAAAAAGAA GCTGGTGGAA 
GCCGAGGAAC GCCGCCACTC CCTGGAGAAC AAGGTAAAGA GACTAGAGAC CATGGAGCGT 
AGAGAGAACA GACTGAAGGA TGACATCCAG ACAAAGTCCG AACAGATCCA GCAGATGGCT 
GATAAAATTC TGGAGCTGGA GGAGAAACAT CGGGAGGCTC AGGTCTCAGC TCAACATCTA 
GAAGTACACT TGAAACAGAA AGAACAGCAC TACGAGGAAA AGATCAAGGT ATTGGACAAT 
CAGATAAAGA AGGACCTGGC CGACAAGGAG AGCCTGGAGA ACATGATGCA GAGACACGAG 
GAGGAGGCCC ACGAGAAGGG CAAGATCCTC AGCGAGCAGA AGGCGATGAT CAACGCGATG 
GATTCCAAGA TCCGATCCCT GGAGCAGAGG ATCGTGGAGC TGTCGGAAGC CAACAAGCTT 
GCGGCAAACA GCAGTCTCTT CACCCAGAGG AACATGAAGG CCCAGGAAGA GATGATCTCA 
GAACTCAGGC AGCAGAAATT TTACCTGGAG ACGCAGGCCG GAAAGCTGGA GGCCCAGAAC 
CGAAAGCTGG AAGAGCAACT GGAGAAGATC AGCCACCAAG ATCACAGTGA CAAGAGTCGG 
CTGCTGGAGC TGGAAACAAG GCTGAGGGAG GTCAGCCTGG AGCACGAGGA GCAGAAGCTG 
GAGCTGAAGC GGCAGCTCAC GGAGCTGCAG CTGTCCCTGC AGGAGCGCGA GTCCCAGCTG 
ACGGCCCTGC AGGCCGCCCG GGCAGCGCTG GAGAGCCAGC TCCGCCAGGC GAAGACAGAG 
CTGGAGGAGA CAACCGCGGA AGCGGAGGAG GAGATCCAGG CGCTCACGGC ACATCGAGAT 
GAGATCCAGC GCAAATTCGA TGCCCTTCGC AACAGCTGCA CGGTCATCAC CGACCTGGAG 
GAGCAGCTGA ACCAGCTCAC CGAGGACAAC GCCGAGCTCA ACAACCAAAA CTTCTACCTG 
TCCAAACAAC TCGATGAGGC TTCCGGGGCC AATGACGAGA TTGTGCAGCT GCGGAGTGAG 
GTGGACCATC TCCGCCGTGA GATCACGGAG CGGGAGATGC AGCTCACCAG CCAGAAGCAA 
ACGATGGAGG CTCTGAAGAC GACATGCACC ATGCTGGAGG AGCAGGTCCT GGACCTGGAG 
GCCCTGAACG ACGAGCTGCT GGAGAAGGAG CGCCAGTGGG AGGCCTGGCG GAGCGTCCTC 
GGCGACGAGA AGTCCCAGTT CGAGTGTCGA GTTCGAGAGC TACAGAGGAT GCTGGACACC 
GAGAAGCAGA GCAGGGCTAG GGCCGATCAG CGGATCACCG AGTCGCGCCA GGTGGTGGAG 
TTGGCGGTGA AGGAACACAA GGCCGAGATT CTTGCTCTGC AGCAGGCTCT CAAGGAGCAG 
AAGCTCAAAG CCGAGAGCCT GTCGGACAAG CTCAACGACC TGGAGAAGAA GCACGCCATG 
CTGGAGATGA ACGCCCGGAG CTTACAGCAG AAACTAGAGA CAGAGCGGGA GCTCAAACAG 
AGGCTTCTGG AGGAGCAAGC CAAATTGCAG CAGCAGATGG ACCTGCAGAA GAACCACATC 
TTCAGACTGA CGCAAGGGCT GCAGGAGGCG CTGGACCGGG CGGATCTGCT GAAGACAGAA 
AGGAGCGACC TGGAGTACCA GCTGGAAAAC ATTCAGGTTC TCTACTCTCA CGAGAAAGTG 
AAAATGGAAG GCACAATCTC TCAGCAAACC AAACTCATTG ATTTCCTGCA AGCCAAAATG 
GACCAGCCTG CTAAAAAGAA AAAGGGTTTA TTTAGTCGAC GGAAAGAGGA CCCTGCTTTG 
CCCACACAGG TTCCTCTGCA GTACAATGAG CTGAAGCTAG CCCTGGAAAA GGAGAAAGCC 
CGATGCGCGG AGCTGGAGGA GGCCCTTCAG AAGACCCGCA TCGAACTCCG CTCTGCGCGG 
GAGGAAGCTG CCCACCGCAA AGCCACGGAC CACCCGCACC CGTCTACGCC AGCCACTGCG 
AGGCAGCAGA TCGCCATGTC TGCCATTGTG CGGTCGCCCG AGCACCAGCC CAGTGCCATG 
AGCCTGCTTG CCCCACCATC CAGCCGCAGA AAGGAGTCGT CAACTCCAGA GGAATTCAGC 
CGGCGTCTGA AAGAGCGCAT GCACCACAAC ATCCCTCACC GGTTTAATGT GGGCCTGAAC 
ATGAGAGCCA CCAAGTGCGC CGTGTGTCTG GATACTGTGC ACTTTGGACG CCAGGCATCC 
AAATGCCTCG AATGTCAAGT GATGTGTCAC CCCAAATGCT CCACCTGCTT GCCCGCTACC 
TGTGGCCTGC CAGCTGAATA TGCCACACAC TTCACTGAGG CCTTCTGCCG GGACAAAATG 
AACTCCCCGG GGCTCCAGAG CAAGGAGCCT GGCAGCAGCT TGCACCTGGA AGGGTGGATG 
AAAGTGCCCA GAAATAACAA ACGGGGACAG CAAGGCTGGG ACAGGAAGTA CATTGTTCTG 
GAGGGGTCAA AAGTCCTCAT CTATGACAAT GAAGCCAGAG AAGCTGGACA GAGGCCGGTG 
GAAGAATTTG AGCTGTGCCT TCCTGACGGG GACGTATCTA TTCATGGTGC CGTTGGTGCT 
TCAGAACTTG CAAATACCGC CAAAGCAGAT GTCCCATACA TCCTGAAGAT GGAGTCTCAC 
CCACACACCA CCTGCTGGCC TGGGAGAACC CTCTACTTGC TAGCACCCAG CTTCCCCGAC 
AAGCAGCGCT GGGTCACCGC CTTAGAATCT GTCGTCGCAG GTGGGAGAGT TTCTAGGGAA 
AAGGCCGAAG CCGATGCTGC ACGTGACCAT ACTTCGAGTG AGCATCAGCC AGTGTGGGTT 
GAGAAATTAC TTGGAAACTC TCTGCTGAAA CTGGAGGGCG ATGACCGGCT TGACATGAAC 
TGCACCCTGC CCTTCAGTGA CCAGGTAGTG CTGGTGGGCA CGGAGGAAGG CCTCTACGCC 
CTGAATGTCT TGAAAAACTC CTTAACCCAC ATCCCAGGGA TTGGCGCAGT CTTCCAAATT 
TACATCATCA AGGACCTGGA GAAGCTGCTC ATGATAGCAG GGGAAGAGCG GGCTCTGTGC 
CTGGTGGACG TGAAGAAGGT GAAGCAGTCC CTGGCGCAGT CACACCTGCC TGCCCAGCCC 
GACGTCTCCC CCAACATATT CGAAGCCGTC AAAGGCTGCC ACTTGTTCGC TGCTGGCAAG 
ATCGAGAACA GCCTGTGCAT CTGCGCCGCT ATGCCAAGCA AAGTCGTCAT CCTCCGCTAC 
AATGACAACC TCAGCAAGTA CTGCATCCGC AAGGAGATCG AGACCTCAGA GCCCTGCAGC 
TGTATCCACT TCACCAACTA CAGCATCCTC ATTGGGACCA ACAAATTCTA TGAGATCGAC 
ATGAAGCAGT ACACGCTTGA CGAGTTCCTG GACAAGAACG ACCATTCCTT GGCACCTGCT 
GTGTTCGCCT CCTCGTCCAA CAGCTTCCCT GTCTCCATTG TGCAGGCGAA CAGCGCCGGG 
CAGCGAGAAG AATACCTGCT GTGCTTCCAC GAATTTGGGG TGTTCGTGGA TTCTTACGGA 
AGACGTAGCC GCACAGATGA TCTCAAGTGG AGTCGCTTAC CTCTGGCCTT CGCCTACAGA 
GAACCTTATC TGTTTGTGAC TCACTTCAAC TCCCTGGAAG TCATTGAGAT CCAGGCCAGA 
TCCTCACTGG GGAGCCCTGC CCGAGCATAT CTGGAAATTC CAAACCCTCG CTACCTGGGC 
CCCGCGATTT CCTCCGGAGC GATTTACCTG GCCTCCTCAT ACCAGGACAA GTTAAGGGTC 
ATATGCTGCA AAGGAAACCT CGTGAAGGAG TCAGGCACTG AGCAGCACCG GGTGCCCTCC 
ACCTCCCGCA GCAGCCCCAA CAAGCGAGGC CCACCAACAT ACAACGAGCA CATCACCAAA 
CGCGTGGCCT CCAGCCCGGC GCCACCGGAA GGCCCCAGCC ACCCCCGAGA GCCAAGCACA 
CCGCACCGCT ACCGAGACAG AGAGGGTCGG ACAGAGCTGC GCAGGGACAA GTCTCCAGGC 
CGCCCTCTGG AGCGGGAGAA GTCCCCAGGC CGAATGCTCA GCACTAGGAG AGAGCGGTCC 
CCAGGGAGAC TGTTTGAAGA CAGCAGCAGG GGCCGGCTGC CTGCAGGAGC AGTGAGGACC 
CCACTGTCCC AGGTTAACAA GGTCTGGGAC CAGTCTTCAG TATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cit ( XM_006530136.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006530199.1 Mus musculus citron (Cit), transcript variant X7, mRNA.

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CloneID	OMu35777
Clone ID Related Accession (Same CDS sequence)	XM_006530136.3 , XM_006530136.4
Accession Version	XM_006530136.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6327bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	citron Rho-interacting kinase isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000071.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006530136.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTGAAGT TCAAGTATGG TGTGCGGAAC CCGCCGGAGG CCAGTGCCTC CGAGCCCATT 
GCCAGTCGGG CCTCCAGGCT AAATCTCTTC TTCCAGGGGA AACCGCCCCT CATGACTCAA 
CAGCAGATGT CTGCTCTTTC CCGGGAAGGG ATGCTAGACG CCCTCTTCGC TCTCTTTGAA 
GAGTGCAGCC AACCCGCCCT GATGAAGATG AAGCACGTGA GCAGCTTTGT CCAGAAGTAT 
TCCGACACCA TAGCCGAGTT GCGGGAGCTG CAGCCGTCGG CGAGAGACTT CGAAGTTCGA 
AGCCTTGTGG GCTGTGGTCA CTTCGCTGAA GTGCAGGTGG TTAGAGAGAA GGCGACCGGG 
GACGTCTATG CCATGAAAAT CATGAAGAAG AAGGCTTTGC TGGCCCAGGA ACAGGTTTCA 
TTTTTCGAGG AGGAGAGGAA CATATTATCT CGGAGCACGA GTCCTTGGAT CCCCCAGTTA 
CAGTACGCCT TTCAGGACAA AAATAACCTT TACCTGGTCA TGGAATATCA GCCTGGAGGG 
GATTTGCTGT CGCTTCTGAA CAGATACGAG GACCAATTAG ATGAGAGCAT GATCCAGTTT 
TACCTTGCTG AGCTGATTTT GGCTGTCCAC AGCGTGCACC AGATGGGATA TGTGCATCGA 
GACATCAAGC CCGAGAACAT CCTCATCGAC CGGACGGGAC ACATCAAGCT GGTGGATTTT 
GGATCAGCCG CTAAGATGAA TTCAAATAAG GTGGATGCCA AACTCCCCAT TGGGACCCCG 
GATTACATGG CTCCGGAAGT GTTGACCGTG ATGAACGAGG ACCGAAGGGG CACATACGGC 
TTGGACTGTG ACTGGTGGTC TGTCGGAGTT GTTGCTTATG AGATGGTTTA TGGGAAGACC 
CCATTCACAG AGGGAACCTC CGCCCGGACC TTCAACAACA TCATGAACTT CCAGCGGTTT 
TTGAAGTTCC CAGATGACCC CAAAGTTAGC AGTGAGCTCC TTGATCTGCT TCAGAGTCTG 
CTGTGTGTCC AGAAAGAGAG ACTGAAGTTC GAGGGTCTCT GCTGCCACCC TTTCTTTGCC 
AGAACGGACT GGAACAACAT CCGTAACTCT CCTCCCCCCT TCGTCCCCAC CCTCAAGTCT 
GACGATGACA CCTCCAATTT TGATGAACCA GAGAAGAATT CGTGGGTTTC ATCCTCTGTG 
TGCCAGCTGA GCCCCTCGGG CTTCTCAGGC GAAGAGCTGC CGTTTGTGGG ATTTTCGTAC 
AGCAAGGCAC TGGGGTATCT TGGTAGATCT GAGTCTGTCG TGTCGAGTCT GGACTCCCCT 
GCCAAGGTTA GCTCCATGGA AAAGAAACTT CTCATCAAAA GCAAAGAGCT CCAAGACTCC 
CAGGACAAGT GTCACAAGAT GGAGCAGGAA ATGACCCGGT TACATCGCAG AGTGTCAGAG 
GTGGAGGCTG TGCTTAGTCA GAAGGAGGTG GAGCTGAAGG CCTCTGAGAC TCAGAGATCC 
CTCCTGGAGC AGGACCTTGC TACCTACATC ACAGAATGCA GTAGCTTAAA GCGAAGTTTG 
GAGCAAGCGC GGATGGAGGT GTCCCAGGAG GATGACAAAG CTCTGCAGCT TCTCCACGAC 
ATCCGAGAGC AGAGCCGGAA GCTCCAGGAG ATCAAGGAGC AGGAGTACCA GGCTCAGGTG 
GAGGAGATGA GGCTGATGAT GAATCAGCTG GAAGAAGACC TGGTGTCAGC CCGCAGACGC 
AGCGATCTCT ACGAGTCTGA GCTGAGGGAG TCTCGGCTTG CCGCCGAGGA ATTCAAGCGG 
AAGGCAAACG AATGTCAGCA CAAACTGATG AAGGTAGTCA GCCACCCTCC AAGAGGGGAC 
TCCGGGGGCA CGGCCCTGGA TGATCTTCAC AAGATGCAAG GCCACGCTGG CTTAACGAGT 
GCTAAGGACC AAGGGAAGCC TGAAGTGGGA GAATATTCCA AACTGGAGAA GATCAATGCT 
GAGCAGCAGC TGAAGATCCA GGAGCTCCAG GAGAAGCTGG AGAAGGCTGT AAAAGCCAGC 
ACAGAGGCCA CCGAGCTCCT GCAGAATATC CGCCAGGCAA AGGAGCGAGC AGAGCGGGAG 
CTAGAGAAGC TACACAACCG GGAAGACTCC TCCGAGGGCA TCAAAAAGAA GCTGGTGGAA 
GCCGAGGAAC GCCGCCACTC CCTGGAGAAC AAGGTAAAGA GACTAGAGAC CATGGAGCGT 
AGAGAGAACA GACTGAAGGA TGACATCCAG ACAAAGTCCG AACAGATCCA GCAGATGGCT 
GATAAAATTC TGGAGCTGGA GGAGAAACAT CGGGAGGCTC AGGTCTCAGC TCAACATCTA 
GAAGTACACT TGAAACAGAA AGAACAGCAC TACGAGGAAA AGATCAAGGT ATTGGACAAT 
CAGATAAAGA AGGACCTGGC CGACAAGGAG AGCCTGGAGA ACATGATGCA GAGACACGAG 
GAGGAGGCCC ACGAGAAGGG CAAGATCCTC AGCGAGCAGA AGGCGATGAT CAACGCGATG 
GATTCCAAGA TCCGATCCCT GGAGCAGAGG ATCGTGGAGC TGTCGGAAGC CAACAAGCTT 
GCGGCAAACA GCAGTCTCTT CACCCAGAGG AACATGAAGG CCCAGGAAGA GATGATCTCA 
GAACTCAGGC AGCAGAAATT TTACCTGGAG ACGCAGGCCG GAAAGCTGGA GGCCCAGAAC 
CGAAAGCTGG AAGAGCAACT GGAGAAGATC AGCCACCAAG ATCACAGTGA CAAGAGTCGG 
CTGCTGGAGC TGGAAACAAG GCTGAGGGAG GTCAGCCTGG AGCACGAGGA GCAGAAGCTG 
GAGCTGAAGC GGCAGCTCAC GGAGCTGCAG CTGTCCCTGC AGGAGCGCGA GTCCCAGCTG 
ACGGCCCTGC AGGCCGCCCG GGCAGCGCTG GAGAGCCAGC TCCGCCAGGC GAAGACAGAG 
CTGGAGGAGA CAACCGCGGA AGCGGAGGAG GAGATCCAGG CGCTCACGGC ACATCGAGAT 
GAGATCCAGC GCAAATTCGA TGCCCTTCGC AACAGCTGCA CGGTCATCAC CGACCTGGAG 
GAGCAGCTGA ACCAGCTCAC CGAGGACAAC GCCGAGCTCA ACAACCAAAA CTTCTACCTG 
TCCAAACAAC TCGATGAGGC TTCCGGGGCC AATGACGAGA TTGTGCAGCT GCGGAGTGAG 
GTGGACCATC TCCGCCGTGA GATCACGGAG CGGGAGATGC AGCTCACCAG CCAGAAGCAA 
GCACAACTCA GTGCTCCTGA TTTGCAGACG ATGGAGGCTC TGAAGACGAC ATGCACCATG 
CTGGAGGAGC AGGTCCTGGA CCTGGAGGCC CTGAACGACG AGCTGCTGGA GAAGGAGCGC 
CAGTGGGAGG CCTGGCGGAG CGTCCTCGGC GACGAGAAGT CCCAGTTCGA GTGTCGAGTT 
CGAGAGCTAC AGAGGATGCT GGACACCGAG AAGCAGAGCA GGGCTAGGGC CGATCAGCGG 
ATCACCGAGT CGCGCCAGGT GGTGGAGTTG GCGGTGAAGG AACACAAGGC CGAGATTCTT 
GCTCTGCAGC AGGCTCTCAA GGAGCAGAAG CTCAAAGCCG AGAGCCTGTC GGACAAGCTC 
AACGACCTGG AGAAGAAGCA CGCCATGCTG GAGATGAACG CCCGGAGCTT ACAGCAGAAA 
CTAGAGACAG AGCGGGAGCT CAAACAGAGG CTTCTGGAGG AGCAAGCCAA ATTGCAGCAG 
CAGATGGACC TGCAGAAGAA CCACATCTTC AGACTGACGC AAGGGCTGCA GGAGGCGCTG 
GACCGGGCGG ATCTGCTGAA GACAGAAAGG AGCGACCTGG AGTACCAGCT GGAAAACATT 
CAGGTTCTCT ACTCTCACGA GAAAGTGAAA ATGGAAGGCA CAATCTCTCA GCAAACCAAA 
CTCATTGATT TCCTGCAAGC CAAAATGGAC CAGCCTGCTA AAAAGAAAAA GGTTCCTCTG 
CAGTACAATG AGCTGAAGCT AGCCCTGGAA AAGGAGAAAG CCCGATGCGC GGAGCTGGAG 
GAGGCCCTTC AGAAGACCCG CATCGAACTC CGCTCTGCGC GGGAGGAAGC TGCCCACCGC 
AAAGCCACGG ACCACCCGCA CCCGTCTACG CCAGCCACTG CGAGGCAGCA GATCGCCATG 
TCTGCCATTG TGCGGTCGCC CGAGCACCAG CCCAGTGCCA TGAGCCTGCT TGCCCCACCA 
TCCAGCCGCA GAAAGGAGTC GTCAACTCCA GAGGAATTCA GCCGGCGTCT GAAAGAGCGC 
ATGCACCACA ACATCCCTCA CCGGTTTAAT GTGGGCCTGA ACATGAGAGC CACCAAGTGC 
GCCGTGTGTC TGGATACTGT GCACTTTGGA CGCCAGGCAT CCAAATGCCT CGAATGTCAA 
GTGATGTGTC ACCCCAAATG CTCCACCTGC TTGCCCGCTA CCTGTGGCCT GCCAGCTGAA 
TATGCCACAC ACTTCACTGA GGCCTTCTGC CGGGACAAAA TGAACTCCCC GGGGCTCCAG 
AGCAAGGAGC CTGGCAGCAG CTTGCACCTG GAAGGGTGGA TGAAAGTGCC CAGAAATAAC 
AAACGGGGAC AGCAAGGCTG GGACAGGAAG TACATTGTTC TGGAGGGGTC AAAAGTCCTC 
ATCTATGACA ATGAAGCCAG AGAAGCTGGA CAGAGGCCGG TGGAAGAATT TGAGCTGTGC 
CTTCCTGACG GGGACGTATC TATTCATGGT GCCGTTGGTG CTTCAGAACT TGCAAATACC 
GCCAAAGCAG ATGTCCCATA CATCCTGAAG ATGGAGTCTC ACCCACACAC CACCTGCTGG 
CCTGGGAGAA CCCTCTACTT GCTAGCACCC AGCTTCCCCG ACAAGCAGCG CTGGGTCACC 
GCCTTAGAAT CTGTCGTCGC AGGTGGGAGA GTTTCTAGGG AAAAGGCCGA AGCCGATGCT 
GCACGTGACC ATACTTCGAG TGAGCATCAG CCAGTGTGGG TTGAGAAATT ACTTGGAAAC 
TCTCTGCTGA AACTGGAGGG CGATGACCGG CTTGACATGA ACTGCACCCT GCCCTTCAGT 
GACCAGGTAG TGCTGGTGGG CACGGAGGAA GGCCTCTACG CCCTGAATGT CTTGAAAAAC 
TCCTTAACCC ACATCCCAGG GATTGGCGCA GTCTTCCAAA TTTACATCAT CAAGGACCTG 
GAGAAGCTGC TCATGATAGC AGGGGAAGAG CGGGCTCTGT GCCTGGTGGA CGTGAAGAAG 
GTGAAGCAGT CCCTGGCGCA GTCACACCTG CCTGCCCAGC CCGACGTCTC CCCCAACATA 
TTCGAAGCCG TCAAAGGCTG CCACTTGTTC GCTGCTGGCA AGATCGAGAA CAGCCTGTGC 
ATCTGCGCCG CTATGCCAAG CAAAGTCGTC ATCCTCCGCT ACAATGACAA CCTCAGCAAG 
TACTGCATCC GCAAGGAGAT CGAGACCTCA GAGCCCTGCA GCTGTATCCA CTTCACCAAC 
TACAGCATCC TCATTGGGAC CAACAAATTC TATGAGATCG ACATGAAGCA GTACACGCTT 
GACGAGTTCC TGGACAAGAA CGACCATTCC TTGGCACCTG CTGTGTTCGC CTCCTCGTCC 
AACAGCTTCC CTGTCTCCAT TGTGCAGGCG AACAGCGCCG GGCAGCGAGA AGAATACCTG 
CTGTGCTTCC ACGAATTTGG GGTGTTCGTG GATTCTTACG GAAGACGTAG CCGCACAGAT 
GATCTCAAGT GGAGTCGCTT ACCTCTGGCC TTCGCCTACA GAGAACCTTA TCTGTTTGTG 
ACTCACTTCA ACTCCCTGGA AGTCATTGAG ATCCAGGCCA GATCCTCACT GGGGAGCCCT 
GCCCGAGCAT ATCTGGAAAT TCCAAACCCT CGCTACCTGG GCCCCGCGAT TTCCTCCGGA 
GCGATTTACC TGGCCTCCTC ATACCAGGAC AAGTTAAGGG TCATATGCTG CAAAGGAAAC 
CTCGTGAAGG AGTCAGGCAC TGAGCAGCAC CGGGTGCCCT CCACCTCCCG CAGCAGCCCC 
AACAAGCGAG GCCCACCAAC ATACAACGAG CACATCACCA AACGCGTGGC CTCCAGCCCG 
GCGCCACCGG AAGGCCCCAG CCACCCCCGA GAGCCAAGCA CACCGCACCG CTACCGAGAC 
AGAGAGGGTC GGACAGAGCT GCGCAGGGAC AAGTCTCCAG GCCGCCCTCT GGAGCGGGAG 
AAGTCCCCAG GCCGAATGCT CAGCACTAGG AGAGAGCGGT CCCCAGGGAG ACTGTTTGAA 
GACAGCAGCA GGGGCCGGCT GCCTGCAGGA GCAGTGAGGA CCCCACTGTC CCAGGTTAAC 
AAGGTCTGGG ACCAGTCTTC AGTATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006530199.1
CDS	158..6484
Translation	MLKFKYGVRNPPEASASEPIASRASRLNLFFQGKPPLMTQQQMSALSREGMLDALFALFEECSQPALMKMKHVSSFVQKYSDTIAELRELQPSARDFEVRSLVGCGHFAEVQVVREKATGDVYAMKIMKKKALLAQEQVSFFEEERNILSRSTSPWIPQLQYAFQDKNNLYLVMEYQPGGDLLSLLNRYEDQLDESMIQFYLAELILAVHSVHQMGYVHRDIKPENILIDRTGHIKLVDFGSAAKMNSNKVDAKLPIGTPDYMAPEVLTVMNEDRRGTYGLDCDWWSVGVVAYEMVYGKTPFTEGTSARTFNNIMNFQRFLKFPDDPKVSSELLDLLQSLLCVQKERLKFEGLCCHPFFARTDWNNIRNSPPPFVPTLKSDDDTSNFDEPEKNSWVSSSVCQLSPSGFSGEELPFVGFSYSKALGYLGRSESVVSSLDSPAKVSSMEKKLLIKSKELQDSQDKCHKMEQEMTRLHRRVSEVEAVLSQKEVELKASETQRSLLEQDLATYITECSSLKRSLEQARMEVSQEDDKALQLLHDIREQSRKLQEIKEQEYQAQVEEMRLMMNQLEEDLVSARRRSDLYESELRESRLAAEEFKRKANECQHKLMKVVSHPPRGDSGGTALDDLHKMQGHAGLTSAKDQGKPEVGEYSKLEKINAEQQLKIQELQEKLEKAVKASTEATELLQNIRQAKERAERELEKLHNREDSSEGIKKKLVEAEERRHSLENKVKRLETMERRENRLKDDIQTKSEQIQQMADKILELEEKHREAQVSAQHLEVHLKQKEQHYEEKIKVLDNQIKKDLADKESLENMMQRHEEEAHEKGKILSEQKAMINAMDSKIRSLEQRIVELSEANKLAANSSLFTQRNMKAQEEMISELRQQKFYLETQAGKLEAQNRKLEEQLEKISHQDHSDKSRLLELETRLREVSLEHEEQKLELKRQLTELQLSLQERESQLTALQAARAALESQLRQAKTELEETTAEAEEEIQALTAHRDEIQRKFDALRNSCTVITDLEEQLNQLTEDNAELNNQNFYLSKQLDEASGANDEIVQLRSEVDHLRREITEREMQLTSQKQAQLSAPDLQTMEALKTTCTMLEEQVLDLEALNDELLEKERQWEAWRSVLGDEKSQFECRVRELQRMLDTEKQSRARADQRITESRQVVELAVKEHKAEILALQQALKEQKLKAESLSDKLNDLEKKHAMLEMNARSLQQKLETERELKQRLLEEQAKLQQQMDLQKNHIFRLTQGLQEALDRADLLKTERSDLEYQLENIQVLYSHEKVKMEGTISQQTKLIDFLQAKMDQPAKKKKVPLQYNELKLALEKEKARCAELEEALQKTRIELRSAREEAAHRKATDHPHPSTPATARQQIAMSAIVRSPEHQPSAMSLLAPPSSRRKESSTPEEFSRRLKERMHHNIPHRFNVGLNMRATKCAVCLDTVHFGRQASKCLECQVMCHPKCSTCLPATCGLPAEYATHFTEAFCRDKMNSPGLQSKEPGSSLHLEGWMKVPRNNKRGQQGWDRKYIVLEGSKVLIYDNEAREAGQRPVEEFELCLPDGDVSIHGAVGASELANTAKADVPYILKMESHPHTTCWPGRTLYLLAPSFPDKQRWVTALESVVAGGRVSREKAEADAARDHTSSEHQPVWVEKLLGNSLLKLEGDDRLDMNCTLPFSDQVVLVGTEEGLYALNVLKNSLTHIPGIGAVFQIYIIKDLEKLLMIAGEERALCLVDVKKVKQSLAQSHLPAQPDVSPNIFEAVKGCHLFAAGKIENSLCICAAMPSKVVILRYNDNLSKYCIRKEIETSEPCSCIHFTNYSILIGTNKFYEIDMKQYTLDEFLDKNDHSLAPAVFASSSNSFPVSIVQANSAGQREEYLLCFHEFGVFVDSYGRRSRTDDLKWSRLPLAFAYREPYLFVTHFNSLEVIEIQARSSLGSPARAYLEIPNPRYLGPAISSGAIYLASSYQDKLRVICCKGNLVKESGTEQHRVPSTSRSSPNKRGPPTYNEHITKRVASSPAPPEGPSHPREPSTPHRYRDREGRTELRRDKSPGRPLEREKSPGRMLSTRRERSPGRLFEDSSRGRLPAGAVRTPLSQVNKVWDQSSV

Target ORF information:

RefSeq Version	XM_006530136.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus citron (Cit), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006530136.3

ORF Insert Sequence:

ATGTTGAAGT TCAAGTATGG TGTGCGGAAC CCGCCGGAGG CCAGTGCCTC CGAGCCCATT 
GCCAGTCGGG CCTCCAGGCT AAATCTCTTC TTCCAGGGGA AACCGCCCCT CATGACTCAA 
CAGCAGATGT CTGCTCTTTC CCGGGAAGGG ATGCTAGACG CCCTCTTCGC TCTCTTTGAA 
GAGTGCAGCC AACCCGCCCT GATGAAGATG AAGCACGTGA GCAGCTTTGT CCAGAAGTAT 
TCCGACACCA TAGCCGAGTT GCGGGAGCTG CAGCCGTCGG CGAGAGACTT CGAAGTTCGA 
AGCCTTGTGG GCTGTGGTCA CTTCGCTGAA GTGCAGGTGG TTAGAGAGAA GGCGACCGGG 
GACGTCTATG CCATGAAAAT CATGAAGAAG AAGGCTTTGC TGGCCCAGGA ACAGGTTTCA 
TTTTTCGAGG AGGAGAGGAA CATATTATCT CGGAGCACGA GTCCTTGGAT CCCCCAGTTA 
CAGTACGCCT TTCAGGACAA AAATAACCTT TACCTGGTCA TGGAATATCA GCCTGGAGGG 
GATTTGCTGT CGCTTCTGAA CAGATACGAG GACCAATTAG ATGAGAGCAT GATCCAGTTT 
TACCTTGCTG AGCTGATTTT GGCTGTCCAC AGCGTGCACC AGATGGGATA TGTGCATCGA 
GACATCAAGC CCGAGAACAT CCTCATCGAC CGGACGGGAC ACATCAAGCT GGTGGATTTT 
GGATCAGCCG CTAAGATGAA TTCAAATAAG GTGGATGCCA AACTCCCCAT TGGGACCCCG 
GATTACATGG CTCCGGAAGT GTTGACCGTG ATGAACGAGG ACCGAAGGGG CACATACGGC 
TTGGACTGTG ACTGGTGGTC TGTCGGAGTT GTTGCTTATG AGATGGTTTA TGGGAAGACC 
CCATTCACAG AGGGAACCTC CGCCCGGACC TTCAACAACA TCATGAACTT CCAGCGGTTT 
TTGAAGTTCC CAGATGACCC CAAAGTTAGC AGTGAGCTCC TTGATCTGCT TCAGAGTCTG 
CTGTGTGTCC AGAAAGAGAG ACTGAAGTTC GAGGGTCTCT GCTGCCACCC TTTCTTTGCC 
AGAACGGACT GGAACAACAT CCGTAACTCT CCTCCCCCCT TCGTCCCCAC CCTCAAGTCT 
GACGATGACA CCTCCAATTT TGATGAACCA GAGAAGAATT CGTGGGTTTC ATCCTCTGTG 
TGCCAGCTGA GCCCCTCGGG CTTCTCAGGC GAAGAGCTGC CGTTTGTGGG ATTTTCGTAC 
AGCAAGGCAC TGGGGTATCT TGGTAGATCT GAGTCTGTCG TGTCGAGTCT GGACTCCCCT 
GCCAAGGTTA GCTCCATGGA AAAGAAACTT CTCATCAAAA GCAAAGAGCT CCAAGACTCC 
CAGGACAAGT GTCACAAGAT GGAGCAGGAA ATGACCCGGT TACATCGCAG AGTGTCAGAG 
GTGGAGGCTG TGCTTAGTCA GAAGGAGGTG GAGCTGAAGG CCTCTGAGAC TCAGAGATCC 
CTCCTGGAGC AGGACCTTGC TACCTACATC ACAGAATGCA GTAGCTTAAA GCGAAGTTTG 
GAGCAAGCGC GGATGGAGGT GTCCCAGGAG GATGACAAAG CTCTGCAGCT TCTCCACGAC 
ATCCGAGAGC AGAGCCGGAA GCTCCAGGAG ATCAAGGAGC AGGAGTACCA GGCTCAGGTG 
GAGGAGATGA GGCTGATGAT GAATCAGCTG GAAGAAGACC TGGTGTCAGC CCGCAGACGC 
AGCGATCTCT ACGAGTCTGA GCTGAGGGAG TCTCGGCTTG CCGCCGAGGA ATTCAAGCGG 
AAGGCAAACG AATGTCAGCA CAAACTGATG AAGGTAGTCA GCCACCCTCC AAGAGGGGAC 
TCCGGGGGCA CGGCCCTGGA TGATCTTCAC AAGATGCAAG GCCACGCTGG CTTAACGAGT 
GCTAAGGACC AAGGGAAGCC TGAAGTGGGA GAATATTCCA AACTGGAGAA GATCAATGCT 
GAGCAGCAGC TGAAGATCCA GGAGCTCCAG GAGAAGCTGG AGAAGGCTGT AAAAGCCAGC 
ACAGAGGCCA CCGAGCTCCT GCAGAATATC CGCCAGGCAA AGGAGCGAGC AGAGCGGGAG 
CTAGAGAAGC TACACAACCG GGAAGACTCC TCCGAGGGCA TCAAAAAGAA GCTGGTGGAA 
GCCGAGGAAC GCCGCCACTC CCTGGAGAAC AAGGTAAAGA GACTAGAGAC CATGGAGCGT 
AGAGAGAACA GACTGAAGGA TGACATCCAG ACAAAGTCCG AACAGATCCA GCAGATGGCT 
GATAAAATTC TGGAGCTGGA GGAGAAACAT CGGGAGGCTC AGGTCTCAGC TCAACATCTA 
GAAGTACACT TGAAACAGAA AGAACAGCAC TACGAGGAAA AGATCAAGGT ATTGGACAAT 
CAGATAAAGA AGGACCTGGC CGACAAGGAG AGCCTGGAGA ACATGATGCA GAGACACGAG 
GAGGAGGCCC ACGAGAAGGG CAAGATCCTC AGCGAGCAGA AGGCGATGAT CAACGCGATG 
GATTCCAAGA TCCGATCCCT GGAGCAGAGG ATCGTGGAGC TGTCGGAAGC CAACAAGCTT 
GCGGCAAACA GCAGTCTCTT CACCCAGAGG AACATGAAGG CCCAGGAAGA GATGATCTCA 
GAACTCAGGC AGCAGAAATT TTACCTGGAG ACGCAGGCCG GAAAGCTGGA GGCCCAGAAC 
CGAAAGCTGG AAGAGCAACT GGAGAAGATC AGCCACCAAG ATCACAGTGA CAAGAGTCGG 
CTGCTGGAGC TGGAAACAAG GCTGAGGGAG GTCAGCCTGG AGCACGAGGA GCAGAAGCTG 
GAGCTGAAGC GGCAGCTCAC GGAGCTGCAG CTGTCCCTGC AGGAGCGCGA GTCCCAGCTG 
ACGGCCCTGC AGGCCGCCCG GGCAGCGCTG GAGAGCCAGC TCCGCCAGGC GAAGACAGAG 
CTGGAGGAGA CAACCGCGGA AGCGGAGGAG GAGATCCAGG CGCTCACGGC ACATCGAGAT 
GAGATCCAGC GCAAATTCGA TGCCCTTCGC AACAGCTGCA CGGTCATCAC CGACCTGGAG 
GAGCAGCTGA ACCAGCTCAC CGAGGACAAC GCCGAGCTCA ACAACCAAAA CTTCTACCTG 
TCCAAACAAC TCGATGAGGC TTCCGGGGCC AATGACGAGA TTGTGCAGCT GCGGAGTGAG 
GTGGACCATC TCCGCCGTGA GATCACGGAG CGGGAGATGC AGCTCACCAG CCAGAAGCAA 
GCACAACTCA GTGCTCCTGA TTTGCAGACG ATGGAGGCTC TGAAGACGAC ATGCACCATG 
CTGGAGGAGC AGGTCCTGGA CCTGGAGGCC CTGAACGACG AGCTGCTGGA GAAGGAGCGC 
CAGTGGGAGG CCTGGCGGAG CGTCCTCGGC GACGAGAAGT CCCAGTTCGA GTGTCGAGTT 
CGAGAGCTAC AGAGGATGCT GGACACCGAG AAGCAGAGCA GGGCTAGGGC CGATCAGCGG 
ATCACCGAGT CGCGCCAGGT GGTGGAGTTG GCGGTGAAGG AACACAAGGC CGAGATTCTT 
GCTCTGCAGC AGGCTCTCAA GGAGCAGAAG CTCAAAGCCG AGAGCCTGTC GGACAAGCTC 
AACGACCTGG AGAAGAAGCA CGCCATGCTG GAGATGAACG CCCGGAGCTT ACAGCAGAAA 
CTAGAGACAG AGCGGGAGCT CAAACAGAGG CTTCTGGAGG AGCAAGCCAA ATTGCAGCAG 
CAGATGGACC TGCAGAAGAA CCACATCTTC AGACTGACGC AAGGGCTGCA GGAGGCGCTG 
GACCGGGCGG ATCTGCTGAA GACAGAAAGG AGCGACCTGG AGTACCAGCT GGAAAACATT 
CAGGTTCTCT ACTCTCACGA GAAAGTGAAA ATGGAAGGCA CAATCTCTCA GCAAACCAAA 
CTCATTGATT TCCTGCAAGC CAAAATGGAC CAGCCTGCTA AAAAGAAAAA GGTTCCTCTG 
CAGTACAATG AGCTGAAGCT AGCCCTGGAA AAGGAGAAAG CCCGATGCGC GGAGCTGGAG 
GAGGCCCTTC AGAAGACCCG CATCGAACTC CGCTCTGCGC GGGAGGAAGC TGCCCACCGC 
AAAGCCACGG ACCACCCGCA CCCGTCTACG CCAGCCACTG CGAGGCAGCA GATCGCCATG 
TCTGCCATTG TGCGGTCGCC CGAGCACCAG CCCAGTGCCA TGAGCCTGCT TGCCCCACCA 
TCCAGCCGCA GAAAGGAGTC GTCAACTCCA GAGGAATTCA GCCGGCGTCT GAAAGAGCGC 
ATGCACCACA ACATCCCTCA CCGGTTTAAT GTGGGCCTGA ACATGAGAGC CACCAAGTGC 
GCCGTGTGTC TGGATACTGT GCACTTTGGA CGCCAGGCAT CCAAATGCCT CGAATGTCAA 
GTGATGTGTC ACCCCAAATG CTCCACCTGC TTGCCCGCTA CCTGTGGCCT GCCAGCTGAA 
TATGCCACAC ACTTCACTGA GGCCTTCTGC CGGGACAAAA TGAACTCCCC GGGGCTCCAG 
AGCAAGGAGC CTGGCAGCAG CTTGCACCTG GAAGGGTGGA TGAAAGTGCC CAGAAATAAC 
AAACGGGGAC AGCAAGGCTG GGACAGGAAG TACATTGTTC TGGAGGGGTC AAAAGTCCTC 
ATCTATGACA ATGAAGCCAG AGAAGCTGGA CAGAGGCCGG TGGAAGAATT TGAGCTGTGC 
CTTCCTGACG GGGACGTATC TATTCATGGT GCCGTTGGTG CTTCAGAACT TGCAAATACC 
GCCAAAGCAG ATGTCCCATA CATCCTGAAG ATGGAGTCTC ACCCACACAC CACCTGCTGG 
CCTGGGAGAA CCCTCTACTT GCTAGCACCC AGCTTCCCCG ACAAGCAGCG CTGGGTCACC 
GCCTTAGAAT CTGTCGTCGC AGGTGGGAGA GTTTCTAGGG AAAAGGCCGA AGCCGATGCT 
GCACGTGACC ATACTTCGAG TGAGCATCAG CCAGTGTGGG TTGAGAAATT ACTTGGAAAC 
TCTCTGCTGA AACTGGAGGG CGATGACCGG CTTGACATGA ACTGCACCCT GCCCTTCAGT 
GACCAGGTAG TGCTGGTGGG CACGGAGGAA GGCCTCTACG CCCTGAATGT CTTGAAAAAC 
TCCTTAACCC ACATCCCAGG GATTGGCGCA GTCTTCCAAA TTTACATCAT CAAGGACCTG 
GAGAAGCTGC TCATGATAGC AGGGGAAGAG CGGGCTCTGT GCCTGGTGGA CGTGAAGAAG 
GTGAAGCAGT CCCTGGCGCA GTCACACCTG CCTGCCCAGC CCGACGTCTC CCCCAACATA 
TTCGAAGCCG TCAAAGGCTG CCACTTGTTC GCTGCTGGCA AGATCGAGAA CAGCCTGTGC 
ATCTGCGCCG CTATGCCAAG CAAAGTCGTC ATCCTCCGCT ACAATGACAA CCTCAGCAAG 
TACTGCATCC GCAAGGAGAT CGAGACCTCA GAGCCCTGCA GCTGTATCCA CTTCACCAAC 
TACAGCATCC TCATTGGGAC CAACAAATTC TATGAGATCG ACATGAAGCA GTACACGCTT 
GACGAGTTCC TGGACAAGAA CGACCATTCC TTGGCACCTG CTGTGTTCGC CTCCTCGTCC 
AACAGCTTCC CTGTCTCCAT TGTGCAGGCG AACAGCGCCG GGCAGCGAGA AGAATACCTG 
CTGTGCTTCC ACGAATTTGG GGTGTTCGTG GATTCTTACG GAAGACGTAG CCGCACAGAT 
GATCTCAAGT GGAGTCGCTT ACCTCTGGCC TTCGCCTACA GAGAACCTTA TCTGTTTGTG 
ACTCACTTCA ACTCCCTGGA AGTCATTGAG ATCCAGGCCA GATCCTCACT GGGGAGCCCT 
GCCCGAGCAT ATCTGGAAAT TCCAAACCCT CGCTACCTGG GCCCCGCGAT TTCCTCCGGA 
GCGATTTACC TGGCCTCCTC ATACCAGGAC AAGTTAAGGG TCATATGCTG CAAAGGAAAC 
CTCGTGAAGG AGTCAGGCAC TGAGCAGCAC CGGGTGCCCT CCACCTCCCG CAGCAGCCCC 
AACAAGCGAG GCCCACCAAC ATACAACGAG CACATCACCA AACGCGTGGC CTCCAGCCCG 
GCGCCACCGG AAGGCCCCAG CCACCCCCGA GAGCCAAGCA CACCGCACCG CTACCGAGAC 
AGAGAGGGTC GGACAGAGCT GCGCAGGGAC AAGTCTCCAG GCCGCCCTCT GGAGCGGGAG 
AAGTCCCCAG GCCGAATGCT CAGCACTAGG AGAGAGCGGT CCCCAGGGAG ACTGTTTGAA 
GACAGCAGCA GGGGCCGGCT GCCTGCAGGA GCAGTGAGGA CCCCACTGTC CCAGGTTAAC 
AAGGTCTGGG ACCAGTCTTC AGTATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cit ( XM_006530137.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006530200.1 Mus musculus citron (Cit), transcript variant X8, mRNA.

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CloneID	OMu35778
Clone ID Related Accession (Same CDS sequence)	XM_006530137.4 , XM_006530137.3
Accession Version	XM_006530137.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6327bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	citron Rho-interacting kinase isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000071.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006530137.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTGAAGT TCAAGTATGG TGTGCGGAAC CCGCCGGAGG CCAGTGCCTC CGAGCCCATT 
GCCAGTCGGG CCTCCAGGCT AAATCTCTTC TTCCAGGGGA AACCGCCCCT CATGACTCAA 
CAGCAGATGT CTGCTCTTTC CCGGGAAGGG ATGCTAGACG CCCTCTTCGC TCTCTTTGAA 
GAGTGCAGCC AACCCGCCCT GATGAAGATG AAGCACGTGA GCAGCTTTGT CCAGAAGTAT 
TCCGACACCA TAGCCGAGTT GCGGGAGCTG CAGCCGTCGG CGAGAGACTT CGAAGTTCGA 
AGCCTTGTGG GCTGTGGTCA CTTCGCTGAA GTGCAGGTGG TTAGAGAGAA GGCGACCGGG 
GACGTCTATG CCATGAAAAT CATGAAGAAG AAGGCTTTGC TGGCCCAGGA ACAGGTTTCA 
TTTTTCGAGG AGGAGAGGAA CATATTATCT CGGAGCACGA GTCCTTGGAT CCCCCAGTTA 
CAGTACGCCT TTCAGGACAA AAATAACCTT TACCTGGTCA TGGAATATCA GCCTGGAGGG 
GATTTGCTGT CGCTTCTGAA CAGATACGAG GACCAATTAG ATGAGAGCAT GATCCAGTTT 
TACCTTGCTG AGCTGATTTT GGCTGTCCAC AGCGTGCACC AGATGGGATA TGTGCATCGA 
GACATCAAGC CCGAGAACAT CCTCATCGAC CGGACGGGAC ACATCAAGCT GGTGGATTTT 
GGATCAGCCG CTAAGATGAA TTCAAATAAG GTGGATGCCA AACTCCCCAT TGGGACCCCG 
GATTACATGG CTCCGGAAGT GTTGACCGTG ATGAACGAGG ACCGAAGGGG CACATACGGC 
TTGGACTGTG ACTGGTGGTC TGTCGGAGTT GTTGCTTATG AGATGGTTTA TGGGAAGACC 
CCATTCACAG AGGGAACCTC CGCCCGGACC TTCAACAACA TCATGAACTT CCAGCGGTTT 
TTGAAGTTCC CAGATGACCC CAAAGTTAGC AGTGAGCTCC TTGATCTGCT TCAGAGTCTG 
CTGTGTGTCC AGAAAGAGAG ACTGAAGTTC GAGGGTCTCT GCTGCCACCC TTTCTTTGCC 
AGAACGGACT GGAACAACAT CCGTAACTCT CCTCCCCCCT TCGTCCCCAC CCTCAAGTCT 
GACGATGACA CCTCCAATTT TGATGAACCA GAGAAGAATT CGTGGGTTTC ATCCTCTGTG 
TGCCAGCTGA GCCCCTCGGG CTTCTCAGGC GAAGAGCTGC CGTTTGTGGG ATTTTCGTAC 
AGCAAGGCAC TGGGGTATCT TGGTAGATCT GAGTCTGTCG TGTCGAGTCT GGACTCCCCT 
GCCAAGGTTA GCTCCATGGA AAAGAAACTT CTCATCAAAA GCAAAGAGCT CCAAGACTCC 
CAGGACAAGT GTCACAAGAT GGAGCAGGAA ATGACCCGGT TACATCGCAG AGTGTCAGAG 
GTGGAGGCTG TGCTTAGTCA GAAGGAGGTG GAGCTGAAGG CCTCTGAGAC TCAGAGATCC 
CTCCTGGAGC AGGACCTTGC TACCTACATC ACAGAATGCA GTAGCTTAAA GCGAAGTTTG 
GAGCAAGCGC GGATGGAGGT GTCCCAGGAG GATGACAAAG CTCTGCAGCT TCTCCACGAC 
ATCCGAGAGC AGAGCCGGAA GCTCCAGGAG ATCAAGGAGC AGGAGTACCA GGCTCAGGTG 
GAGGAGATGA GGCTGATGAT GAATCAGCTG GAAGAAGACC TGGTGTCAGC CCGCAGACGC 
AGCGATCTCT ACGAGTCTGA GCTGAGGGAG TCTCGGCTTG CCGCCGAGGA ATTCAAGCGG 
AAGGCAAACG AATGTCAGCA CAAACTGATG AAGGTAGTCA GCCACCCTCC AAGAGGGGAC 
TCCGGGGGCA CGGCCCTGGA TGATCTTCAC AAGATGCAAG GCCACGCTGG CTTAACGAGT 
GCTAAGGACC AAGGGAAGCC TGAAGTGGGA GAATATTCCA AACTGGAGAA GATCAATGCT 
GAGCAGCAGC TGAAGATCCA GGAGCTCCAG GAGAAGCTGG AGAAGGCTGT AAAAGCCAGC 
ACAGAGGCCA CCGAGCTCCT GCAGAATATC CGCCAGGCAA AGGAGCGAGC AGAGCGGGAG 
CTAGAGAAGC TACACAACCG GGAAGACTCC TCCGAGGGCA TCAAAAAGAA GCTGGTGGAA 
GCCGAGGAAC GCCGCCACTC CCTGGAGAAC AAGGTAAAGA GACTAGAGAC CATGGAGCGT 
AGAGAGAACA GACTGAAGGA TGACATCCAG ACAAAGTCCG AACAGATCCA GCAGATGGCT 
GATAAAATTC TGGAGCTGGA GGAGAAACAT CGGGAGGCTC AGGTCTCAGC TCAACATCTA 
GAAGTACACT TGAAACAGAA AGAACAGCAC TACGAGGAAA AGATCAAGGT ATTGGACAAT 
CAGATAAAGA AGGACCTGGC CGACAAGGAG AGCCTGGAGA ACATGATGCA GAGACACGAG 
GAGGAGGCCC ACGAGAAGGG CAAGATCCTC AGCGAGCAGA AGGCGATGAT CAACGCGATG 
GATTCCAAGA TCCGATCCCT GGAGCAGAGG ATCGTGGAGC TGTCGGAAGC CAACAAGCTT 
GCGGCAAACA GCAGTCTCTT CACCCAGAGG AACATGAAGG CCCAGGAAGA GATGATCTCA 
GAACTCAGGC AGCAGAAATT TTACCTGGAG ACGCAGGCCG GAAAGCTGGA GGCCCAGAAC 
CGAAAGCTGG AAGAGCAACT GGAGAAGATC AGCCACCAAG ATCACAGTGA CAAGAGTCGG 
CTGCTGGAGC TGGAAACAAG GCTGAGGGAG GTCAGCCTGG AGCACGAGGA GCAGAAGCTG 
GAGCTGAAGC GGCAGCTCAC GGAGCTGCAG CTGTCCCTGC AGGAGCGCGA GTCCCAGCTG 
ACGGCCCTGC AGGCCGCCCG GGCAGCGCTG GAGAGCCAGC TCCGCCAGGC GAAGACAGAG 
CTGGAGGAGA CAACCGCGGA AGCGGAGGAG GAGATCCAGG CGCTCACGGC ACATCGAGAT 
GAGATCCAGC GCAAATTCGA TGCCCTTCGC AACAGCTGCA CGGTCATCAC CGACCTGGAG 
GAGCAGCTGA ACCAGCTCAC CGAGGACAAC GCCGAGCTCA ACAACCAAAA CTTCTACCTG 
TCCAAACAAC TCGATGAGGC TTCCGGGGCC AATGACGAGA TTGTGCAGCT GCGGAGTGAG 
GTGGACCATC TCCGCCGTGA GATCACGGAG CGGGAGATGC AGCTCACCAG CCAGAAGCAA 
GCACAACTCA GTGCTCCTGA TTTGCAGACG ATGGAGGCTC TGAAGACGAC ATGCACCATG 
CTGGAGGAGC AGGTCCTGGA CCTGGAGGCC CTGAACGACG AGCTGCTGGA GAAGGAGCGC 
CAGTGGGAGG CCTGGCGGAG CGTCCTCGGC GACGAGAAGT CCCAGTTCGA GTGTCGAGTT 
CGAGAGCTAC AGAGGATGCT GGACACCGAG AAGCAGAGCA GGGCTAGGGC CGATCAGCGG 
ATCACCGAGT CGCGCCAGGT GGTGGAGTTG GCGGTGAAGG AACACAAGGC CGAGATTCTT 
GCTCTGCAGC AGGCTCTCAA GGAGCAGAAG CTCAAAGCCG AGAGCCTGTC GGACAAGCTC 
AACGACCTGG AGAAGAAGCA CGCCATGCTG GAGATGAACG CCCGGAGCTT ACAGCAGAAA 
CTAGAGACAG AGCGGGAGCT CAAACAGAGG CTTCTGGAGG AGCAAGCCAA ATTGCAGCAG 
CAGATGGACC TGCAGAAGAA CCACATCTTC AGACTGACGC AAGGGCTGCA GGAGGCGCTG 
GACCGGGCGG ATCTGCTGAA GACAGAAAGG AGCGACCTGG AGTACCAGCT GGAAAACATT 
CAGGTTCTCT ACTCTCACGA GAAAGTGAAA ATGGAAGGCA CAATCTCTCA GCAAACCAAA 
CTCATTGATT TCCTGCAAGC CAAAATGGAC CAGCCTGCTA AAAAGAAAAA GGGTTTATTT 
AGTCGACGGA AAGAGGACCC TGCTTTGCCC ACACAGGTTC CTCTGCAGTA CAATGAGCTG 
AAGCTAGCCC TGGAAAAGGA GAAAGCCCGA TGCGCGGAGC TGGAGGAGGC CCTTCAGAAG 
ACCCGCATCG AACTCCGCTC TGCGCGGGAG GAAGCTGCCC ACCGCAAAGC CACGGACCAC 
CCGCACCCGT CTACGCCAGC CACTGCGAGG CAGCAGATCG CCATGTCTGC CATTGTGCGG 
TCGCCCGAGC ACCAGCCCAG TGCCATGAGC CTGCTTGCCC CACCATCCAG CCGCAGAAAG 
GAGTCGTCAA CTCCAGAGGA ATTCAGCCGG CGTCTGAAAG AGCGCATGCA CCACAACATC 
CCTCACCGGT TTAATGTGGG CCTGAACATG AGAGCCACCA AGTGCGCCGT GTGTCTGGAT 
ACTGTGCACT TTGGACGCCA GGCATCCAAA TGCCTCGAAT GTCAAGTGAT GTGTCACCCC 
AAATGCTCCA CCTGCTTGCC CGCTACCTGT GGCCTGCCAG CTGAATATGC CACACACTTC 
ACTGAGGCCT TCTGCCGGGA CAAAATGAAC TCCCCGGGGC TCCAGAGCAA GGAGCCTGGC 
AGCAGCTTGC ACCTGGAAGG GTGGATGAAA GTGCCCAGAA ATAACAAACG GGGACAGCAA 
GGCTGGGACA GGAAGTACAT TGTTCTGGAG GGGTCAAAAG TCCTCATCTA TGACAATGAA 
GCCAGAGAAG CTGGACAGAG GCCGGTGGAA GAATTTGAGC TGTGCCTTCC TGACGGGGAC 
GTATCTATTC ATGGTGCCGT TGGTGCTTCA GAACTTGCAA ATACCGCCAA AGCAGATGTC 
CCATACATCC TGAAGATGGA GTCTCACCCA CACACCACCT GCTGGCCTGG GAGAACCCTC 
TACTTGCTAG CACCCAGCTT CCCCGACAAG CAGCGCTGGG TCACCGCCTT AGAATCTGTC 
GTCGCAGGTG GGAGAGTTTC TAGGGAAAAG GCCGAAGCCG ATGCTAAATT ACTTGGAAAC 
TCTCTGCTGA AACTGGAGGG CGATGACCGG CTTGACATGA ACTGCACCCT GCCCTTCAGT 
GACCAGGTAG TGCTGGTGGG CACGGAGGAA GGCCTCTACG CCCTGAATGT CTTGAAAAAC 
TCCTTAACCC ACATCCCAGG GATTGGCGCA GTCTTCCAAA TTTACATCAT CAAGGACCTG 
GAGAAGCTGC TCATGATAGC AGGGGAAGAG CGGGCTCTGT GCCTGGTGGA CGTGAAGAAG 
GTGAAGCAGT CCCTGGCGCA GTCACACCTG CCTGCCCAGC CCGACGTCTC CCCCAACATA 
TTCGAAGCCG TCAAAGGCTG CCACTTGTTC GCTGCTGGCA AGATCGAGAA CAGCCTGTGC 
ATCTGCGCCG CTATGCCAAG CAAAGTCGTC ATCCTCCGCT ACAATGACAA CCTCAGCAAG 
TACTGCATCC GCAAGGAGAT CGAGACCTCA GAGCCCTGCA GCTGTATCCA CTTCACCAAC 
TACAGCATCC TCATTGGGAC CAACAAATTC TATGAGATCG ACATGAAGCA GTACACGCTT 
GACGAGTTCC TGGACAAGAA CGACCATTCC TTGGCACCTG CTGTGTTCGC CTCCTCGTCC 
AACAGCTTCC CTGTCTCCAT TGTGCAGGCG AACAGCGCCG GGCAGCGAGA AGAATACCTG 
CTGTGCTTCC ACGAATTTGG GGTGTTCGTG GATTCTTACG GAAGACGTAG CCGCACAGAT 
GATCTCAAGT GGAGTCGCTT ACCTCTGGCC TTCGCCTACA GAGAACCTTA TCTGTTTGTG 
ACTCACTTCA ACTCCCTGGA AGTCATTGAG ATCCAGGCCA GATCCTCACT GGGGAGCCCT 
GCCCGAGCAT ATCTGGAAAT TCCAAACCCT CGCTACCTGG GCCCCGCGAT TTCCTCCGGA 
GCGATTTACC TGGCCTCCTC ATACCAGGAC AAGTTAAGGG TCATATGCTG CAAAGGAAAC 
CTCGTGAAGG AGTCAGGCAC TGAGCAGCAC CGGGTGCCCT CCACCTCCCG CAGCAGCCCC 
AACAAGCGAG GCCCACCAAC ATACAACGAG CACATCACCA AACGCGTGGC CTCCAGCCCG 
GCGCCACCGG AAGGCCCCAG CCACCCCCGA GAGCCAAGCA CACCGCACCG CTACCGAGAC 
AGAGAGGGTC GGACAGAGCT GCGCAGGGAC AAGTCTCCAG GCCGCCCTCT GGAGCGGGAG 
AAGTCCCCAG GCCGAATGCT CAGCACTAGG AGAGAGCGGT CCCCAGGGAG ACTGTTTGAA 
GACAGCAGCA GGGGCCGGCT GCCTGCAGGA GCAGTGAGGA CCCCACTGTC CCAGGTTAAC 
AAGGTCTGGG ACCAGTCTTC AGTATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006530200.1
CDS	158..6484
Translation	MLKFKYGVRNPPEASASEPIASRASRLNLFFQGKPPLMTQQQMSALSREGMLDALFALFEECSQPALMKMKHVSSFVQKYSDTIAELRELQPSARDFEVRSLVGCGHFAEVQVVREKATGDVYAMKIMKKKALLAQEQVSFFEEERNILSRSTSPWIPQLQYAFQDKNNLYLVMEYQPGGDLLSLLNRYEDQLDESMIQFYLAELILAVHSVHQMGYVHRDIKPENILIDRTGHIKLVDFGSAAKMNSNKVDAKLPIGTPDYMAPEVLTVMNEDRRGTYGLDCDWWSVGVVAYEMVYGKTPFTEGTSARTFNNIMNFQRFLKFPDDPKVSSELLDLLQSLLCVQKERLKFEGLCCHPFFARTDWNNIRNSPPPFVPTLKSDDDTSNFDEPEKNSWVSSSVCQLSPSGFSGEELPFVGFSYSKALGYLGRSESVVSSLDSPAKVSSMEKKLLIKSKELQDSQDKCHKMEQEMTRLHRRVSEVEAVLSQKEVELKASETQRSLLEQDLATYITECSSLKRSLEQARMEVSQEDDKALQLLHDIREQSRKLQEIKEQEYQAQVEEMRLMMNQLEEDLVSARRRSDLYESELRESRLAAEEFKRKANECQHKLMKVVSHPPRGDSGGTALDDLHKMQGHAGLTSAKDQGKPEVGEYSKLEKINAEQQLKIQELQEKLEKAVKASTEATELLQNIRQAKERAERELEKLHNREDSSEGIKKKLVEAEERRHSLENKVKRLETMERRENRLKDDIQTKSEQIQQMADKILELEEKHREAQVSAQHLEVHLKQKEQHYEEKIKVLDNQIKKDLADKESLENMMQRHEEEAHEKGKILSEQKAMINAMDSKIRSLEQRIVELSEANKLAANSSLFTQRNMKAQEEMISELRQQKFYLETQAGKLEAQNRKLEEQLEKISHQDHSDKSRLLELETRLREVSLEHEEQKLELKRQLTELQLSLQERESQLTALQAARAALESQLRQAKTELEETTAEAEEEIQALTAHRDEIQRKFDALRNSCTVITDLEEQLNQLTEDNAELNNQNFYLSKQLDEASGANDEIVQLRSEVDHLRREITEREMQLTSQKQAQLSAPDLQTMEALKTTCTMLEEQVLDLEALNDELLEKERQWEAWRSVLGDEKSQFECRVRELQRMLDTEKQSRARADQRITESRQVVELAVKEHKAEILALQQALKEQKLKAESLSDKLNDLEKKHAMLEMNARSLQQKLETERELKQRLLEEQAKLQQQMDLQKNHIFRLTQGLQEALDRADLLKTERSDLEYQLENIQVLYSHEKVKMEGTISQQTKLIDFLQAKMDQPAKKKKGLFSRRKEDPALPTQVPLQYNELKLALEKEKARCAELEEALQKTRIELRSAREEAAHRKATDHPHPSTPATARQQIAMSAIVRSPEHQPSAMSLLAPPSSRRKESSTPEEFSRRLKERMHHNIPHRFNVGLNMRATKCAVCLDTVHFGRQASKCLECQVMCHPKCSTCLPATCGLPAEYATHFTEAFCRDKMNSPGLQSKEPGSSLHLEGWMKVPRNNKRGQQGWDRKYIVLEGSKVLIYDNEAREAGQRPVEEFELCLPDGDVSIHGAVGASELANTAKADVPYILKMESHPHTTCWPGRTLYLLAPSFPDKQRWVTALESVVAGGRVSREKAEADAKLLGNSLLKLEGDDRLDMNCTLPFSDQVVLVGTEEGLYALNVLKNSLTHIPGIGAVFQIYIIKDLEKLLMIAGEERALCLVDVKKVKQSLAQSHLPAQPDVSPNIFEAVKGCHLFAAGKIENSLCICAAMPSKVVILRYNDNLSKYCIRKEIETSEPCSCIHFTNYSILIGTNKFYEIDMKQYTLDEFLDKNDHSLAPAVFASSSNSFPVSIVQANSAGQREEYLLCFHEFGVFVDSYGRRSRTDDLKWSRLPLAFAYREPYLFVTHFNSLEVIEIQARSSLGSPARAYLEIPNPRYLGPAISSGAIYLASSYQDKLRVICCKGNLVKESGTEQHRVPSTSRSSPNKRGPPTYNEHITKRVASSPAPPEGPSHPREPSTPHRYRDREGRTELRRDKSPGRPLEREKSPGRMLSTRRERSPGRLFEDSSRGRLPAGAVRTPLSQVNKVWDQSSV

Target ORF information:

RefSeq Version	XM_006530137.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus citron (Cit), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006530137.3

ORF Insert Sequence:

ATGTTGAAGT TCAAGTATGG TGTGCGGAAC CCGCCGGAGG CCAGTGCCTC CGAGCCCATT 
GCCAGTCGGG CCTCCAGGCT AAATCTCTTC TTCCAGGGGA AACCGCCCCT CATGACTCAA 
CAGCAGATGT CTGCTCTTTC CCGGGAAGGG ATGCTAGACG CCCTCTTCGC TCTCTTTGAA 
GAGTGCAGCC AACCCGCCCT GATGAAGATG AAGCACGTGA GCAGCTTTGT CCAGAAGTAT 
TCCGACACCA TAGCCGAGTT GCGGGAGCTG CAGCCGTCGG CGAGAGACTT CGAAGTTCGA 
AGCCTTGTGG GCTGTGGTCA CTTCGCTGAA GTGCAGGTGG TTAGAGAGAA GGCGACCGGG 
GACGTCTATG CCATGAAAAT CATGAAGAAG AAGGCTTTGC TGGCCCAGGA ACAGGTTTCA 
TTTTTCGAGG AGGAGAGGAA CATATTATCT CGGAGCACGA GTCCTTGGAT CCCCCAGTTA 
CAGTACGCCT TTCAGGACAA AAATAACCTT TACCTGGTCA TGGAATATCA GCCTGGAGGG 
GATTTGCTGT CGCTTCTGAA CAGATACGAG GACCAATTAG ATGAGAGCAT GATCCAGTTT 
TACCTTGCTG AGCTGATTTT GGCTGTCCAC AGCGTGCACC AGATGGGATA TGTGCATCGA 
GACATCAAGC CCGAGAACAT CCTCATCGAC CGGACGGGAC ACATCAAGCT GGTGGATTTT 
GGATCAGCCG CTAAGATGAA TTCAAATAAG GTGGATGCCA AACTCCCCAT TGGGACCCCG 
GATTACATGG CTCCGGAAGT GTTGACCGTG ATGAACGAGG ACCGAAGGGG CACATACGGC 
TTGGACTGTG ACTGGTGGTC TGTCGGAGTT GTTGCTTATG AGATGGTTTA TGGGAAGACC 
CCATTCACAG AGGGAACCTC CGCCCGGACC TTCAACAACA TCATGAACTT CCAGCGGTTT 
TTGAAGTTCC CAGATGACCC CAAAGTTAGC AGTGAGCTCC TTGATCTGCT TCAGAGTCTG 
CTGTGTGTCC AGAAAGAGAG ACTGAAGTTC GAGGGTCTCT GCTGCCACCC TTTCTTTGCC 
AGAACGGACT GGAACAACAT CCGTAACTCT CCTCCCCCCT TCGTCCCCAC CCTCAAGTCT 
GACGATGACA CCTCCAATTT TGATGAACCA GAGAAGAATT CGTGGGTTTC ATCCTCTGTG 
TGCCAGCTGA GCCCCTCGGG CTTCTCAGGC GAAGAGCTGC CGTTTGTGGG ATTTTCGTAC 
AGCAAGGCAC TGGGGTATCT TGGTAGATCT GAGTCTGTCG TGTCGAGTCT GGACTCCCCT 
GCCAAGGTTA GCTCCATGGA AAAGAAACTT CTCATCAAAA GCAAAGAGCT CCAAGACTCC 
CAGGACAAGT GTCACAAGAT GGAGCAGGAA ATGACCCGGT TACATCGCAG AGTGTCAGAG 
GTGGAGGCTG TGCTTAGTCA GAAGGAGGTG GAGCTGAAGG CCTCTGAGAC TCAGAGATCC 
CTCCTGGAGC AGGACCTTGC TACCTACATC ACAGAATGCA GTAGCTTAAA GCGAAGTTTG 
GAGCAAGCGC GGATGGAGGT GTCCCAGGAG GATGACAAAG CTCTGCAGCT TCTCCACGAC 
ATCCGAGAGC AGAGCCGGAA GCTCCAGGAG ATCAAGGAGC AGGAGTACCA GGCTCAGGTG 
GAGGAGATGA GGCTGATGAT GAATCAGCTG GAAGAAGACC TGGTGTCAGC CCGCAGACGC 
AGCGATCTCT ACGAGTCTGA GCTGAGGGAG TCTCGGCTTG CCGCCGAGGA ATTCAAGCGG 
AAGGCAAACG AATGTCAGCA CAAACTGATG AAGGTAGTCA GCCACCCTCC AAGAGGGGAC 
TCCGGGGGCA CGGCCCTGGA TGATCTTCAC AAGATGCAAG GCCACGCTGG CTTAACGAGT 
GCTAAGGACC AAGGGAAGCC TGAAGTGGGA GAATATTCCA AACTGGAGAA GATCAATGCT 
GAGCAGCAGC TGAAGATCCA GGAGCTCCAG GAGAAGCTGG AGAAGGCTGT AAAAGCCAGC 
ACAGAGGCCA CCGAGCTCCT GCAGAATATC CGCCAGGCAA AGGAGCGAGC AGAGCGGGAG 
CTAGAGAAGC TACACAACCG GGAAGACTCC TCCGAGGGCA TCAAAAAGAA GCTGGTGGAA 
GCCGAGGAAC GCCGCCACTC CCTGGAGAAC AAGGTAAAGA GACTAGAGAC CATGGAGCGT 
AGAGAGAACA GACTGAAGGA TGACATCCAG ACAAAGTCCG AACAGATCCA GCAGATGGCT 
GATAAAATTC TGGAGCTGGA GGAGAAACAT CGGGAGGCTC AGGTCTCAGC TCAACATCTA 
GAAGTACACT TGAAACAGAA AGAACAGCAC TACGAGGAAA AGATCAAGGT ATTGGACAAT 
CAGATAAAGA AGGACCTGGC CGACAAGGAG AGCCTGGAGA ACATGATGCA GAGACACGAG 
GAGGAGGCCC ACGAGAAGGG CAAGATCCTC AGCGAGCAGA AGGCGATGAT CAACGCGATG 
GATTCCAAGA TCCGATCCCT GGAGCAGAGG ATCGTGGAGC TGTCGGAAGC CAACAAGCTT 
GCGGCAAACA GCAGTCTCTT CACCCAGAGG AACATGAAGG CCCAGGAAGA GATGATCTCA 
GAACTCAGGC AGCAGAAATT TTACCTGGAG ACGCAGGCCG GAAAGCTGGA GGCCCAGAAC 
CGAAAGCTGG AAGAGCAACT GGAGAAGATC AGCCACCAAG ATCACAGTGA CAAGAGTCGG 
CTGCTGGAGC TGGAAACAAG GCTGAGGGAG GTCAGCCTGG AGCACGAGGA GCAGAAGCTG 
GAGCTGAAGC GGCAGCTCAC GGAGCTGCAG CTGTCCCTGC AGGAGCGCGA GTCCCAGCTG 
ACGGCCCTGC AGGCCGCCCG GGCAGCGCTG GAGAGCCAGC TCCGCCAGGC GAAGACAGAG 
CTGGAGGAGA CAACCGCGGA AGCGGAGGAG GAGATCCAGG CGCTCACGGC ACATCGAGAT 
GAGATCCAGC GCAAATTCGA TGCCCTTCGC AACAGCTGCA CGGTCATCAC CGACCTGGAG 
GAGCAGCTGA ACCAGCTCAC CGAGGACAAC GCCGAGCTCA ACAACCAAAA CTTCTACCTG 
TCCAAACAAC TCGATGAGGC TTCCGGGGCC AATGACGAGA TTGTGCAGCT GCGGAGTGAG 
GTGGACCATC TCCGCCGTGA GATCACGGAG CGGGAGATGC AGCTCACCAG CCAGAAGCAA 
GCACAACTCA GTGCTCCTGA TTTGCAGACG ATGGAGGCTC TGAAGACGAC ATGCACCATG 
CTGGAGGAGC AGGTCCTGGA CCTGGAGGCC CTGAACGACG AGCTGCTGGA GAAGGAGCGC 
CAGTGGGAGG CCTGGCGGAG CGTCCTCGGC GACGAGAAGT CCCAGTTCGA GTGTCGAGTT 
CGAGAGCTAC AGAGGATGCT GGACACCGAG AAGCAGAGCA GGGCTAGGGC CGATCAGCGG 
ATCACCGAGT CGCGCCAGGT GGTGGAGTTG GCGGTGAAGG AACACAAGGC CGAGATTCTT 
GCTCTGCAGC AGGCTCTCAA GGAGCAGAAG CTCAAAGCCG AGAGCCTGTC GGACAAGCTC 
AACGACCTGG AGAAGAAGCA CGCCATGCTG GAGATGAACG CCCGGAGCTT ACAGCAGAAA 
CTAGAGACAG AGCGGGAGCT CAAACAGAGG CTTCTGGAGG AGCAAGCCAA ATTGCAGCAG 
CAGATGGACC TGCAGAAGAA CCACATCTTC AGACTGACGC AAGGGCTGCA GGAGGCGCTG 
GACCGGGCGG ATCTGCTGAA GACAGAAAGG AGCGACCTGG AGTACCAGCT GGAAAACATT 
CAGGTTCTCT ACTCTCACGA GAAAGTGAAA ATGGAAGGCA CAATCTCTCA GCAAACCAAA 
CTCATTGATT TCCTGCAAGC CAAAATGGAC CAGCCTGCTA AAAAGAAAAA GGGTTTATTT 
AGTCGACGGA AAGAGGACCC TGCTTTGCCC ACACAGGTTC CTCTGCAGTA CAATGAGCTG 
AAGCTAGCCC TGGAAAAGGA GAAAGCCCGA TGCGCGGAGC TGGAGGAGGC CCTTCAGAAG 
ACCCGCATCG AACTCCGCTC TGCGCGGGAG GAAGCTGCCC ACCGCAAAGC CACGGACCAC 
CCGCACCCGT CTACGCCAGC CACTGCGAGG CAGCAGATCG CCATGTCTGC CATTGTGCGG 
TCGCCCGAGC ACCAGCCCAG TGCCATGAGC CTGCTTGCCC CACCATCCAG CCGCAGAAAG 
GAGTCGTCAA CTCCAGAGGA ATTCAGCCGG CGTCTGAAAG AGCGCATGCA CCACAACATC 
CCTCACCGGT TTAATGTGGG CCTGAACATG AGAGCCACCA AGTGCGCCGT GTGTCTGGAT 
ACTGTGCACT TTGGACGCCA GGCATCCAAA TGCCTCGAAT GTCAAGTGAT GTGTCACCCC 
AAATGCTCCA CCTGCTTGCC CGCTACCTGT GGCCTGCCAG CTGAATATGC CACACACTTC 
ACTGAGGCCT TCTGCCGGGA CAAAATGAAC TCCCCGGGGC TCCAGAGCAA GGAGCCTGGC 
AGCAGCTTGC ACCTGGAAGG GTGGATGAAA GTGCCCAGAA ATAACAAACG GGGACAGCAA 
GGCTGGGACA GGAAGTACAT TGTTCTGGAG GGGTCAAAAG TCCTCATCTA TGACAATGAA 
GCCAGAGAAG CTGGACAGAG GCCGGTGGAA GAATTTGAGC TGTGCCTTCC TGACGGGGAC 
GTATCTATTC ATGGTGCCGT TGGTGCTTCA GAACTTGCAA ATACCGCCAA AGCAGATGTC 
CCATACATCC TGAAGATGGA GTCTCACCCA CACACCACCT GCTGGCCTGG GAGAACCCTC 
TACTTGCTAG CACCCAGCTT CCCCGACAAG CAGCGCTGGG TCACCGCCTT AGAATCTGTC 
GTCGCAGGTG GGAGAGTTTC TAGGGAAAAG GCCGAAGCCG ATGCTAAATT ACTTGGAAAC 
TCTCTGCTGA AACTGGAGGG CGATGACCGG CTTGACATGA ACTGCACCCT GCCCTTCAGT 
GACCAGGTAG TGCTGGTGGG CACGGAGGAA GGCCTCTACG CCCTGAATGT CTTGAAAAAC 
TCCTTAACCC ACATCCCAGG GATTGGCGCA GTCTTCCAAA TTTACATCAT CAAGGACCTG 
GAGAAGCTGC TCATGATAGC AGGGGAAGAG CGGGCTCTGT GCCTGGTGGA CGTGAAGAAG 
GTGAAGCAGT CCCTGGCGCA GTCACACCTG CCTGCCCAGC CCGACGTCTC CCCCAACATA 
TTCGAAGCCG TCAAAGGCTG CCACTTGTTC GCTGCTGGCA AGATCGAGAA CAGCCTGTGC 
ATCTGCGCCG CTATGCCAAG CAAAGTCGTC ATCCTCCGCT ACAATGACAA CCTCAGCAAG 
TACTGCATCC GCAAGGAGAT CGAGACCTCA GAGCCCTGCA GCTGTATCCA CTTCACCAAC 
TACAGCATCC TCATTGGGAC CAACAAATTC TATGAGATCG ACATGAAGCA GTACACGCTT 
GACGAGTTCC TGGACAAGAA CGACCATTCC TTGGCACCTG CTGTGTTCGC CTCCTCGTCC 
AACAGCTTCC CTGTCTCCAT TGTGCAGGCG AACAGCGCCG GGCAGCGAGA AGAATACCTG 
CTGTGCTTCC ACGAATTTGG GGTGTTCGTG GATTCTTACG GAAGACGTAG CCGCACAGAT 
GATCTCAAGT GGAGTCGCTT ACCTCTGGCC TTCGCCTACA GAGAACCTTA TCTGTTTGTG 
ACTCACTTCA ACTCCCTGGA AGTCATTGAG ATCCAGGCCA GATCCTCACT GGGGAGCCCT 
GCCCGAGCAT ATCTGGAAAT TCCAAACCCT CGCTACCTGG GCCCCGCGAT TTCCTCCGGA 
GCGATTTACC TGGCCTCCTC ATACCAGGAC AAGTTAAGGG TCATATGCTG CAAAGGAAAC 
CTCGTGAAGG AGTCAGGCAC TGAGCAGCAC CGGGTGCCCT CCACCTCCCG CAGCAGCCCC 
AACAAGCGAG GCCCACCAAC ATACAACGAG CACATCACCA AACGCGTGGC CTCCAGCCCG 
GCGCCACCGG AAGGCCCCAG CCACCCCCGA GAGCCAAGCA CACCGCACCG CTACCGAGAC 
AGAGAGGGTC GGACAGAGCT GCGCAGGGAC AAGTCTCCAG GCCGCCCTCT GGAGCGGGAG 
AAGTCCCCAG GCCGAATGCT CAGCACTAGG AGAGAGCGGT CCCCAGGGAG ACTGTTTGAA 
GACAGCAGCA GGGGCCGGCT GCCTGCAGGA GCAGTGAGGA CCCCACTGTC CCAGGTTAAC 
AAGGTCTGGG ACCAGTCTTC AGTATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cit ( XM_006530138.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006530201.1 Mus musculus citron (Cit), transcript variant X9, mRNA.

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CloneID	OMu35779
Clone ID Related Accession (Same CDS sequence)	XM_030254099.1 , XM_006530138.3 , XM_006530138.4
Accession Version	XM_006530138.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6285bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	citron Rho-interacting kinase isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000071.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006530138.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTGAAGT TCAAGTATGG TGTGCGGAAC CCGCCGGAGG CCAGTGCCTC CGAGCCCATT 
GCCAGTCGGG CCTCCAGGCT AAATCTCTTC TTCCAGGGGA AACCGCCCCT CATGACTCAA 
CAGCAGATGT CTGCTCTTTC CCGGGAAGGG ATGCTAGACG CCCTCTTCGC TCTCTTTGAA 
GAGTGCAGCC AACCCGCCCT GATGAAGATG AAGCACGTGA GCAGCTTTGT CCAGAAGTAT 
TCCGACACCA TAGCCGAGTT GCGGGAGCTG CAGCCGTCGG CGAGAGACTT CGAAGTTCGA 
AGCCTTGTGG GCTGTGGTCA CTTCGCTGAA GTGCAGGTGG TTAGAGAGAA GGCGACCGGG 
GACGTCTATG CCATGAAAAT CATGAAGAAG AAGGCTTTGC TGGCCCAGGA ACAGGTTTCA 
TTTTTCGAGG AGGAGAGGAA CATATTATCT CGGAGCACGA GTCCTTGGAT CCCCCAGTTA 
CAGTACGCCT TTCAGGACAA AAATAACCTT TACCTGGTCA TGGAATATCA GCCTGGAGGG 
GATTTGCTGT CGCTTCTGAA CAGATACGAG GACCAATTAG ATGAGAGCAT GATCCAGTTT 
TACCTTGCTG AGCTGATTTT GGCTGTCCAC AGCGTGCACC AGATGGGATA TGTGCATCGA 
GACATCAAGC CCGAGAACAT CCTCATCGAC CGGACGGGAC ACATCAAGCT GGTGGATTTT 
GGATCAGCCG CTAAGATGAA TTCAAATAAG GTGGATGCCA AACTCCCCAT TGGGACCCCG 
GATTACATGG CTCCGGAAGT GTTGACCGTG ATGAACGAGG ACCGAAGGGG CACATACGGC 
TTGGACTGTG ACTGGTGGTC TGTCGGAGTT GTTGCTTATG AGATGGTTTA TGGGAAGACC 
CCATTCACAG AGGGAACCTC CGCCCGGACC TTCAACAACA TCATGAACTT CCAGCGGTTT 
TTGAAGTTCC CAGATGACCC CAAAGTTAGC AGTGAGCTCC TTGATCTGCT TCAGAGTCTG 
CTGTGTGTCC AGAAAGAGAG ACTGAAGTTC GAGGGTCTCT GCTGCCACCC TTTCTTTGCC 
AGAACGGACT GGAACAACAT CCGTAACTCT CCTCCCCCCT TCGTCCCCAC CCTCAAGTCT 
GACGATGACA CCTCCAATTT TGATGAACCA GAGAAGAATT CGTGGGTTTC ATCCTCTGTG 
TGCCAGCTGA GCCCCTCGGG CTTCTCAGGC GAAGAGCTGC CGTTTGTGGG ATTTTCGTAC 
AGCAAGGCAC TGGGGTATCT TGGTAGATCT GAGTCTGTCG TGTCGAGTCT GGACTCCCCT 
GCCAAGGTTA GCTCCATGGA AAAGAAACTT CTCATCAAAA GCAAAGAGCT CCAAGACTCC 
CAGGACAAGT GTCACAAGAT GGAGCAGGAA ATGACCCGGT TACATCGCAG AGTGTCAGAG 
GTGGAGGCTG TGCTTAGTCA GAAGGAGGTG GAGCTGAAGG CCTCTGAGAC TCAGAGATCC 
CTCCTGGAGC AGGACCTTGC TACCTACATC ACAGAATGCA GTAGCTTAAA GCGAAGTTTG 
GAGCAAGCGC GGATGGAGGT GTCCCAGGAG GATGACAAAG CTCTGCAGCT TCTCCACGAC 
ATCCGAGAGC AGAGCCGGAA GCTCCAGGAG ATCAAGGAGC AGGAGTACCA GGCTCAGGTG 
GAGGAGATGA GGCTGATGAT GAATCAGCTG GAAGAAGACC TGGTGTCAGC CCGCAGACGC 
AGCGATCTCT ACGAGTCTGA GCTGAGGGAG TCTCGGCTTG CCGCCGAGGA ATTCAAGCGG 
AAGGCAAACG AATGTCAGCA CAAACTGATG AAGGCTAAGG ACCAAGGGAA GCCTGAAGTG 
GGAGAATATT CCAAACTGGA GAAGATCAAT GCTGAGCAGC AGCTGAAGAT CCAGGAGCTC 
CAGGAGAAGC TGGAGAAGGC TGTAAAAGCC AGCACAGAGG CCACCGAGCT CCTGCAGAAT 
ATCCGCCAGG CAAAGGAGCG AGCAGAGCGG GAGCTAGAGA AGCTACACAA CCGGGAAGAC 
TCCTCCGAGG GCATCAAAAA GAAGCTGGTG GAAGCCGAGG AACGCCGCCA CTCCCTGGAG 
AACAAGGTAA AGAGACTAGA GACCATGGAG CGTAGAGAGA ACAGACTGAA GGATGACATC 
CAGACAAAGT CCGAACAGAT CCAGCAGATG GCTGATAAAA TTCTGGAGCT GGAGGAGAAA 
CATCGGGAGG CTCAGGTCTC AGCTCAACAT CTAGAAGTAC ACTTGAAACA GAAAGAACAG 
CACTACGAGG AAAAGATCAA GGTATTGGAC AATCAGATAA AGAAGGACCT GGCCGACAAG 
GAGAGCCTGG AGAACATGAT GCAGAGACAC GAGGAGGAGG CCCACGAGAA GGGCAAGATC 
CTCAGCGAGC AGAAGGCGAT GATCAACGCG ATGGATTCCA AGATCCGATC CCTGGAGCAG 
AGGATCGTGG AGCTGTCGGA AGCCAACAAG CTTGCGGCAA ACAGCAGTCT CTTCACCCAG 
AGGAACATGA AGGCCCAGGA AGAGATGATC TCAGAACTCA GGCAGCAGAA ATTTTACCTG 
GAGACGCAGG CCGGAAAGCT GGAGGCCCAG AACCGAAAGC TGGAAGAGCA ACTGGAGAAG 
ATCAGCCACC AAGATCACAG TGACAAGAGT CGGCTGCTGG AGCTGGAAAC AAGGCTGAGG 
GAGGTCAGCC TGGAGCACGA GGAGCAGAAG CTGGAGCTGA AGCGGCAGCT CACGGAGCTG 
CAGCTGTCCC TGCAGGAGCG CGAGTCCCAG CTGACGGCCC TGCAGGCCGC CCGGGCAGCG 
CTGGAGAGCC AGCTCCGCCA GGCGAAGACA GAGCTGGAGG AGACAACCGC GGAAGCGGAG 
GAGGAGATCC AGGCGCTCAC GGCACATCGA GATGAGATCC AGCGCAAATT CGATGCCCTT 
CGCAACAGCT GCACGGTCAT CACCGACCTG GAGGAGCAGC TGAACCAGCT CACCGAGGAC 
AACGCCGAGC TCAACAACCA AAACTTCTAC CTGTCCAAAC AACTCGATGA GGCTTCCGGG 
GCCAATGACG AGATTGTGCA GCTGCGGAGT GAGGTGGACC ATCTCCGCCG TGAGATCACG 
GAGCGGGAGA TGCAGCTCAC CAGCCAGAAG CAAGCACAAC TCAGTGCTCC TGATTTGCAG 
ACGATGGAGG CTCTGAAGAC GACATGCACC ATGCTGGAGG AGCAGGTCCT GGACCTGGAG 
GCCCTGAACG ACGAGCTGCT GGAGAAGGAG CGCCAGTGGG AGGCCTGGCG GAGCGTCCTC 
GGCGACGAGA AGTCCCAGTT CGAGTGTCGA GTTCGAGAGC TACAGAGGAT GCTGGACACC 
GAGAAGCAGA GCAGGGCTAG GGCCGATCAG CGGATCACCG AGTCGCGCCA GGTGGTGGAG 
TTGGCGGTGA AGGAACACAA GGCCGAGATT CTTGCTCTGC AGCAGGCTCT CAAGGAGCAG 
AAGCTCAAAG CCGAGAGCCT GTCGGACAAG CTCAACGACC TGGAGAAGAA GCACGCCATG 
CTGGAGATGA ACGCCCGGAG CTTACAGCAG AAACTAGAGA CAGAGCGGGA GCTCAAACAG 
AGGCTTCTGG AGGAGCAAGC CAAATTGCAG CAGCAGATGG ACCTGCAGAA GAACCACATC 
TTCAGACTGA CGCAAGGGCT GCAGGAGGCG CTGGACCGGG CGGATCTGCT GAAGACAGAA 
AGGAGCGACC TGGAGTACCA GCTGGAAAAC ATTCAGGTTC TCTACTCTCA CGAGAAAGTG 
AAAATGGAAG GCACAATCTC TCAGCAAACC AAACTCATTG ATTTCCTGCA AGCCAAAATG 
GACCAGCCTG CTAAAAAGAA AAAGGGTTTA TTTAGTCGAC GGAAAGAGGA CCCTGCTTTG 
CCCACACAGG TTCCTCTGCA GTACAATGAG CTGAAGCTAG CCCTGGAAAA GGAGAAAGCC 
CGATGCGCGG AGCTGGAGGA GGCCCTTCAG AAGACCCGCA TCGAACTCCG CTCTGCGCGG 
GAGGAAGCTG CCCACCGCAA AGCCACGGAC CACCCGCACC CGTCTACGCC AGCCACTGCG 
AGGCAGCAGA TCGCCATGTC TGCCATTGTG CGGTCGCCCG AGCACCAGCC CAGTGCCATG 
AGCCTGCTTG CCCCACCATC CAGCCGCAGA AAGGAGTCGT CAACTCCAGA GGAATTCAGC 
CGGCGTCTGA AAGAGCGCAT GCACCACAAC ATCCCTCACC GGTTTAATGT GGGCCTGAAC 
ATGAGAGCCA CCAAGTGCGC CGTGTGTCTG GATACTGTGC ACTTTGGACG CCAGGCATCC 
AAATGCCTCG AATGTCAAGT GATGTGTCAC CCCAAATGCT CCACCTGCTT GCCCGCTACC 
TGTGGCCTGC CAGCTGAATA TGCCACACAC TTCACTGAGG CCTTCTGCCG GGACAAAATG 
AACTCCCCGG GGCTCCAGAG CAAGGAGCCT GGCAGCAGCT TGCACCTGGA AGGGTGGATG 
AAAGTGCCCA GAAATAACAA ACGGGGACAG CAAGGCTGGG ACAGGAAGTA CATTGTTCTG 
GAGGGGTCAA AAGTCCTCAT CTATGACAAT GAAGCCAGAG AAGCTGGACA GAGGCCGGTG 
GAAGAATTTG AGCTGTGCCT TCCTGACGGG GACGTATCTA TTCATGGTGC CGTTGGTGCT 
TCAGAACTTG CAAATACCGC CAAAGCAGAT GTCCCATACA TCCTGAAGAT GGAGTCTCAC 
CCACACACCA CCTGCTGGCC TGGGAGAACC CTCTACTTGC TAGCACCCAG CTTCCCCGAC 
AAGCAGCGCT GGGTCACCGC CTTAGAATCT GTCGTCGCAG GTGGGAGAGT TTCTAGGGAA 
AAGGCCGAAG CCGATGCTGC ACGTGACCAT ACTTCGAGTG AGCATCAGCC AGTGTGGGTT 
GAGAAATTAC TTGGAAACTC TCTGCTGAAA CTGGAGGGCG ATGACCGGCT TGACATGAAC 
TGCACCCTGC CCTTCAGTGA CCAGGTAGTG CTGGTGGGCA CGGAGGAAGG CCTCTACGCC 
CTGAATGTCT TGAAAAACTC CTTAACCCAC ATCCCAGGGA TTGGCGCAGT CTTCCAAATT 
TACATCATCA AGGACCTGGA GAAGCTGCTC ATGATAGCAG GGGAAGAGCG GGCTCTGTGC 
CTGGTGGACG TGAAGAAGGT GAAGCAGTCC CTGGCGCAGT CACACCTGCC TGCCCAGCCC 
GACGTCTCCC CCAACATATT CGAAGCCGTC AAAGGCTGCC ACTTGTTCGC TGCTGGCAAG 
ATCGAGAACA GCCTGTGCAT CTGCGCCGCT ATGCCAAGCA AAGTCGTCAT CCTCCGCTAC 
AATGACAACC TCAGCAAGTA CTGCATCCGC AAGGAGATCG AGACCTCAGA GCCCTGCAGC 
TGTATCCACT TCACCAACTA CAGCATCCTC ATTGGGACCA ACAAATTCTA TGAGATCGAC 
ATGAAGCAGT ACACGCTTGA CGAGTTCCTG GACAAGAACG ACCATTCCTT GGCACCTGCT 
GTGTTCGCCT CCTCGTCCAA CAGCTTCCCT GTCTCCATTG TGCAGGCGAA CAGCGCCGGG 
CAGCGAGAAG AATACCTGCT GTGCTTCCAC GAATTTGGGG TGTTCGTGGA TTCTTACGGA 
AGACGTAGCC GCACAGATGA TCTCAAGTGG AGTCGCTTAC CTCTGGCCTT CGCCTACAGA 
GAACCTTATC TGTTTGTGAC TCACTTCAAC TCCCTGGAAG TCATTGAGAT CCAGGCCAGA 
TCCTCACTGG GGAGCCCTGC CCGAGCATAT CTGGAAATTC CAAACCCTCG CTACCTGGGC 
CCCGCGATTT CCTCCGGAGC GATTTACCTG GCCTCCTCAT ACCAGGACAA GTTAAGGGTC 
ATATGCTGCA AAGGAAACCT CGTGAAGGAG TCAGGCACTG AGCAGCACCG GGTGCCCTCC 
ACCTCCCGCA GCAGCCCCAA CAAGCGAGGC CCACCAACAT ACAACGAGCA CATCACCAAA 
CGCGTGGCCT CCAGCCCGGC GCCACCGGAA GGCCCCAGCC ACCCCCGAGA GCCAAGCACA 
CCGCACCGCT ACCGAGACAG AGAGGGTCGG ACAGAGCTGC GCAGGGACAA GTCTCCAGGC 
CGCCCTCTGG AGCGGGAGAA GTCCCCAGGC CGAATGCTCA GCACTAGGAG AGAGCGGTCC 
CCAGGGAGAC TGTTTGAAGA CAGCAGCAGG GGCCGGCTGC CTGCAGGAGC AGTGAGGACC 
CCACTGTCCC AGGTTAACAA GGTCTGGGAC CAGTCTTCAG TATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006530201.1
CDS	158..6442
Translation	MLKFKYGVRNPPEASASEPIASRASRLNLFFQGKPPLMTQQQMSALSREGMLDALFALFEECSQPALMKMKHVSSFVQKYSDTIAELRELQPSARDFEVRSLVGCGHFAEVQVVREKATGDVYAMKIMKKKALLAQEQVSFFEEERNILSRSTSPWIPQLQYAFQDKNNLYLVMEYQPGGDLLSLLNRYEDQLDESMIQFYLAELILAVHSVHQMGYVHRDIKPENILIDRTGHIKLVDFGSAAKMNSNKVDAKLPIGTPDYMAPEVLTVMNEDRRGTYGLDCDWWSVGVVAYEMVYGKTPFTEGTSARTFNNIMNFQRFLKFPDDPKVSSELLDLLQSLLCVQKERLKFEGLCCHPFFARTDWNNIRNSPPPFVPTLKSDDDTSNFDEPEKNSWVSSSVCQLSPSGFSGEELPFVGFSYSKALGYLGRSESVVSSLDSPAKVSSMEKKLLIKSKELQDSQDKCHKMEQEMTRLHRRVSEVEAVLSQKEVELKASETQRSLLEQDLATYITECSSLKRSLEQARMEVSQEDDKALQLLHDIREQSRKLQEIKEQEYQAQVEEMRLMMNQLEEDLVSARRRSDLYESELRESRLAAEEFKRKANECQHKLMKAKDQGKPEVGEYSKLEKINAEQQLKIQELQEKLEKAVKASTEATELLQNIRQAKERAERELEKLHNREDSSEGIKKKLVEAEERRHSLENKVKRLETMERRENRLKDDIQTKSEQIQQMADKILELEEKHREAQVSAQHLEVHLKQKEQHYEEKIKVLDNQIKKDLADKESLENMMQRHEEEAHEKGKILSEQKAMINAMDSKIRSLEQRIVELSEANKLAANSSLFTQRNMKAQEEMISELRQQKFYLETQAGKLEAQNRKLEEQLEKISHQDHSDKSRLLELETRLREVSLEHEEQKLELKRQLTELQLSLQERESQLTALQAARAALESQLRQAKTELEETTAEAEEEIQALTAHRDEIQRKFDALRNSCTVITDLEEQLNQLTEDNAELNNQNFYLSKQLDEASGANDEIVQLRSEVDHLRREITEREMQLTSQKQAQLSAPDLQTMEALKTTCTMLEEQVLDLEALNDELLEKERQWEAWRSVLGDEKSQFECRVRELQRMLDTEKQSRARADQRITESRQVVELAVKEHKAEILALQQALKEQKLKAESLSDKLNDLEKKHAMLEMNARSLQQKLETERELKQRLLEEQAKLQQQMDLQKNHIFRLTQGLQEALDRADLLKTERSDLEYQLENIQVLYSHEKVKMEGTISQQTKLIDFLQAKMDQPAKKKKGLFSRRKEDPALPTQVPLQYNELKLALEKEKARCAELEEALQKTRIELRSAREEAAHRKATDHPHPSTPATARQQIAMSAIVRSPEHQPSAMSLLAPPSSRRKESSTPEEFSRRLKERMHHNIPHRFNVGLNMRATKCAVCLDTVHFGRQASKCLECQVMCHPKCSTCLPATCGLPAEYATHFTEAFCRDKMNSPGLQSKEPGSSLHLEGWMKVPRNNKRGQQGWDRKYIVLEGSKVLIYDNEAREAGQRPVEEFELCLPDGDVSIHGAVGASELANTAKADVPYILKMESHPHTTCWPGRTLYLLAPSFPDKQRWVTALESVVAGGRVSREKAEADAARDHTSSEHQPVWVEKLLGNSLLKLEGDDRLDMNCTLPFSDQVVLVGTEEGLYALNVLKNSLTHIPGIGAVFQIYIIKDLEKLLMIAGEERALCLVDVKKVKQSLAQSHLPAQPDVSPNIFEAVKGCHLFAAGKIENSLCICAAMPSKVVILRYNDNLSKYCIRKEIETSEPCSCIHFTNYSILIGTNKFYEIDMKQYTLDEFLDKNDHSLAPAVFASSSNSFPVSIVQANSAGQREEYLLCFHEFGVFVDSYGRRSRTDDLKWSRLPLAFAYREPYLFVTHFNSLEVIEIQARSSLGSPARAYLEIPNPRYLGPAISSGAIYLASSYQDKLRVICCKGNLVKESGTEQHRVPSTSRSSPNKRGPPTYNEHITKRVASSPAPPEGPSHPREPSTPHRYRDREGRTELRRDKSPGRPLEREKSPGRMLSTRRERSPGRLFEDSSRGRLPAGAVRTPLSQVNKVWDQSSV

Target ORF information:

RefSeq Version	XM_006530138.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus citron (Cit), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006530138.3

ORF Insert Sequence:

ATGTTGAAGT TCAAGTATGG TGTGCGGAAC CCGCCGGAGG CCAGTGCCTC CGAGCCCATT 
GCCAGTCGGG CCTCCAGGCT AAATCTCTTC TTCCAGGGGA AACCGCCCCT CATGACTCAA 
CAGCAGATGT CTGCTCTTTC CCGGGAAGGG ATGCTAGACG CCCTCTTCGC TCTCTTTGAA 
GAGTGCAGCC AACCCGCCCT GATGAAGATG AAGCACGTGA GCAGCTTTGT CCAGAAGTAT 
TCCGACACCA TAGCCGAGTT GCGGGAGCTG CAGCCGTCGG CGAGAGACTT CGAAGTTCGA 
AGCCTTGTGG GCTGTGGTCA CTTCGCTGAA GTGCAGGTGG TTAGAGAGAA GGCGACCGGG 
GACGTCTATG CCATGAAAAT CATGAAGAAG AAGGCTTTGC TGGCCCAGGA ACAGGTTTCA 
TTTTTCGAGG AGGAGAGGAA CATATTATCT CGGAGCACGA GTCCTTGGAT CCCCCAGTTA 
CAGTACGCCT TTCAGGACAA AAATAACCTT TACCTGGTCA TGGAATATCA GCCTGGAGGG 
GATTTGCTGT CGCTTCTGAA CAGATACGAG GACCAATTAG ATGAGAGCAT GATCCAGTTT 
TACCTTGCTG AGCTGATTTT GGCTGTCCAC AGCGTGCACC AGATGGGATA TGTGCATCGA 
GACATCAAGC CCGAGAACAT CCTCATCGAC CGGACGGGAC ACATCAAGCT GGTGGATTTT 
GGATCAGCCG CTAAGATGAA TTCAAATAAG GTGGATGCCA AACTCCCCAT TGGGACCCCG 
GATTACATGG CTCCGGAAGT GTTGACCGTG ATGAACGAGG ACCGAAGGGG CACATACGGC 
TTGGACTGTG ACTGGTGGTC TGTCGGAGTT GTTGCTTATG AGATGGTTTA TGGGAAGACC 
CCATTCACAG AGGGAACCTC CGCCCGGACC TTCAACAACA TCATGAACTT CCAGCGGTTT 
TTGAAGTTCC CAGATGACCC CAAAGTTAGC AGTGAGCTCC TTGATCTGCT TCAGAGTCTG 
CTGTGTGTCC AGAAAGAGAG ACTGAAGTTC GAGGGTCTCT GCTGCCACCC TTTCTTTGCC 
AGAACGGACT GGAACAACAT CCGTAACTCT CCTCCCCCCT TCGTCCCCAC CCTCAAGTCT 
GACGATGACA CCTCCAATTT TGATGAACCA GAGAAGAATT CGTGGGTTTC ATCCTCTGTG 
TGCCAGCTGA GCCCCTCGGG CTTCTCAGGC GAAGAGCTGC CGTTTGTGGG ATTTTCGTAC 
AGCAAGGCAC TGGGGTATCT TGGTAGATCT GAGTCTGTCG TGTCGAGTCT GGACTCCCCT 
GCCAAGGTTA GCTCCATGGA AAAGAAACTT CTCATCAAAA GCAAAGAGCT CCAAGACTCC 
CAGGACAAGT GTCACAAGAT GGAGCAGGAA ATGACCCGGT TACATCGCAG AGTGTCAGAG 
GTGGAGGCTG TGCTTAGTCA GAAGGAGGTG GAGCTGAAGG CCTCTGAGAC TCAGAGATCC 
CTCCTGGAGC AGGACCTTGC TACCTACATC ACAGAATGCA GTAGCTTAAA GCGAAGTTTG 
GAGCAAGCGC GGATGGAGGT GTCCCAGGAG GATGACAAAG CTCTGCAGCT TCTCCACGAC 
ATCCGAGAGC AGAGCCGGAA GCTCCAGGAG ATCAAGGAGC AGGAGTACCA GGCTCAGGTG 
GAGGAGATGA GGCTGATGAT GAATCAGCTG GAAGAAGACC TGGTGTCAGC CCGCAGACGC 
AGCGATCTCT ACGAGTCTGA GCTGAGGGAG TCTCGGCTTG CCGCCGAGGA ATTCAAGCGG 
AAGGCAAACG AATGTCAGCA CAAACTGATG AAGGCTAAGG ACCAAGGGAA GCCTGAAGTG 
GGAGAATATT CCAAACTGGA GAAGATCAAT GCTGAGCAGC AGCTGAAGAT CCAGGAGCTC 
CAGGAGAAGC TGGAGAAGGC TGTAAAAGCC AGCACAGAGG CCACCGAGCT CCTGCAGAAT 
ATCCGCCAGG CAAAGGAGCG AGCAGAGCGG GAGCTAGAGA AGCTACACAA CCGGGAAGAC 
TCCTCCGAGG GCATCAAAAA GAAGCTGGTG GAAGCCGAGG AACGCCGCCA CTCCCTGGAG 
AACAAGGTAA AGAGACTAGA GACCATGGAG CGTAGAGAGA ACAGACTGAA GGATGACATC 
CAGACAAAGT CCGAACAGAT CCAGCAGATG GCTGATAAAA TTCTGGAGCT GGAGGAGAAA 
CATCGGGAGG CTCAGGTCTC AGCTCAACAT CTAGAAGTAC ACTTGAAACA GAAAGAACAG 
CACTACGAGG AAAAGATCAA GGTATTGGAC AATCAGATAA AGAAGGACCT GGCCGACAAG 
GAGAGCCTGG AGAACATGAT GCAGAGACAC GAGGAGGAGG CCCACGAGAA GGGCAAGATC 
CTCAGCGAGC AGAAGGCGAT GATCAACGCG ATGGATTCCA AGATCCGATC CCTGGAGCAG 
AGGATCGTGG AGCTGTCGGA AGCCAACAAG CTTGCGGCAA ACAGCAGTCT CTTCACCCAG 
AGGAACATGA AGGCCCAGGA AGAGATGATC TCAGAACTCA GGCAGCAGAA ATTTTACCTG 
GAGACGCAGG CCGGAAAGCT GGAGGCCCAG AACCGAAAGC TGGAAGAGCA ACTGGAGAAG 
ATCAGCCACC AAGATCACAG TGACAAGAGT CGGCTGCTGG AGCTGGAAAC AAGGCTGAGG 
GAGGTCAGCC TGGAGCACGA GGAGCAGAAG CTGGAGCTGA AGCGGCAGCT CACGGAGCTG 
CAGCTGTCCC TGCAGGAGCG CGAGTCCCAG CTGACGGCCC TGCAGGCCGC CCGGGCAGCG 
CTGGAGAGCC AGCTCCGCCA GGCGAAGACA GAGCTGGAGG AGACAACCGC GGAAGCGGAG 
GAGGAGATCC AGGCGCTCAC GGCACATCGA GATGAGATCC AGCGCAAATT CGATGCCCTT 
CGCAACAGCT GCACGGTCAT CACCGACCTG GAGGAGCAGC TGAACCAGCT CACCGAGGAC 
AACGCCGAGC TCAACAACCA AAACTTCTAC CTGTCCAAAC AACTCGATGA GGCTTCCGGG 
GCCAATGACG AGATTGTGCA GCTGCGGAGT GAGGTGGACC ATCTCCGCCG TGAGATCACG 
GAGCGGGAGA TGCAGCTCAC CAGCCAGAAG CAAGCACAAC TCAGTGCTCC TGATTTGCAG 
ACGATGGAGG CTCTGAAGAC GACATGCACC ATGCTGGAGG AGCAGGTCCT GGACCTGGAG 
GCCCTGAACG ACGAGCTGCT GGAGAAGGAG CGCCAGTGGG AGGCCTGGCG GAGCGTCCTC 
GGCGACGAGA AGTCCCAGTT CGAGTGTCGA GTTCGAGAGC TACAGAGGAT GCTGGACACC 
GAGAAGCAGA GCAGGGCTAG GGCCGATCAG CGGATCACCG AGTCGCGCCA GGTGGTGGAG 
TTGGCGGTGA AGGAACACAA GGCCGAGATT CTTGCTCTGC AGCAGGCTCT CAAGGAGCAG 
AAGCTCAAAG CCGAGAGCCT GTCGGACAAG CTCAACGACC TGGAGAAGAA GCACGCCATG 
CTGGAGATGA ACGCCCGGAG CTTACAGCAG AAACTAGAGA CAGAGCGGGA GCTCAAACAG 
AGGCTTCTGG AGGAGCAAGC CAAATTGCAG CAGCAGATGG ACCTGCAGAA GAACCACATC 
TTCAGACTGA CGCAAGGGCT GCAGGAGGCG CTGGACCGGG CGGATCTGCT GAAGACAGAA 
AGGAGCGACC TGGAGTACCA GCTGGAAAAC ATTCAGGTTC TCTACTCTCA CGAGAAAGTG 
AAAATGGAAG GCACAATCTC TCAGCAAACC AAACTCATTG ATTTCCTGCA AGCCAAAATG 
GACCAGCCTG CTAAAAAGAA AAAGGGTTTA TTTAGTCGAC GGAAAGAGGA CCCTGCTTTG 
CCCACACAGG TTCCTCTGCA GTACAATGAG CTGAAGCTAG CCCTGGAAAA GGAGAAAGCC 
CGATGCGCGG AGCTGGAGGA GGCCCTTCAG AAGACCCGCA TCGAACTCCG CTCTGCGCGG 
GAGGAAGCTG CCCACCGCAA AGCCACGGAC CACCCGCACC CGTCTACGCC AGCCACTGCG 
AGGCAGCAGA TCGCCATGTC TGCCATTGTG CGGTCGCCCG AGCACCAGCC CAGTGCCATG 
AGCCTGCTTG CCCCACCATC CAGCCGCAGA AAGGAGTCGT CAACTCCAGA GGAATTCAGC 
CGGCGTCTGA AAGAGCGCAT GCACCACAAC ATCCCTCACC GGTTTAATGT GGGCCTGAAC 
ATGAGAGCCA CCAAGTGCGC CGTGTGTCTG GATACTGTGC ACTTTGGACG CCAGGCATCC 
AAATGCCTCG AATGTCAAGT GATGTGTCAC CCCAAATGCT CCACCTGCTT GCCCGCTACC 
TGTGGCCTGC CAGCTGAATA TGCCACACAC TTCACTGAGG CCTTCTGCCG GGACAAAATG 
AACTCCCCGG GGCTCCAGAG CAAGGAGCCT GGCAGCAGCT TGCACCTGGA AGGGTGGATG 
AAAGTGCCCA GAAATAACAA ACGGGGACAG CAAGGCTGGG ACAGGAAGTA CATTGTTCTG 
GAGGGGTCAA AAGTCCTCAT CTATGACAAT GAAGCCAGAG AAGCTGGACA GAGGCCGGTG 
GAAGAATTTG AGCTGTGCCT TCCTGACGGG GACGTATCTA TTCATGGTGC CGTTGGTGCT 
TCAGAACTTG CAAATACCGC CAAAGCAGAT GTCCCATACA TCCTGAAGAT GGAGTCTCAC 
CCACACACCA CCTGCTGGCC TGGGAGAACC CTCTACTTGC TAGCACCCAG CTTCCCCGAC 
AAGCAGCGCT GGGTCACCGC CTTAGAATCT GTCGTCGCAG GTGGGAGAGT TTCTAGGGAA 
AAGGCCGAAG CCGATGCTGC ACGTGACCAT ACTTCGAGTG AGCATCAGCC AGTGTGGGTT 
GAGAAATTAC TTGGAAACTC TCTGCTGAAA CTGGAGGGCG ATGACCGGCT TGACATGAAC 
TGCACCCTGC CCTTCAGTGA CCAGGTAGTG CTGGTGGGCA CGGAGGAAGG CCTCTACGCC 
CTGAATGTCT TGAAAAACTC CTTAACCCAC ATCCCAGGGA TTGGCGCAGT CTTCCAAATT 
TACATCATCA AGGACCTGGA GAAGCTGCTC ATGATAGCAG GGGAAGAGCG GGCTCTGTGC 
CTGGTGGACG TGAAGAAGGT GAAGCAGTCC CTGGCGCAGT CACACCTGCC TGCCCAGCCC 
GACGTCTCCC CCAACATATT CGAAGCCGTC AAAGGCTGCC ACTTGTTCGC TGCTGGCAAG 
ATCGAGAACA GCCTGTGCAT CTGCGCCGCT ATGCCAAGCA AAGTCGTCAT CCTCCGCTAC 
AATGACAACC TCAGCAAGTA CTGCATCCGC AAGGAGATCG AGACCTCAGA GCCCTGCAGC 
TGTATCCACT TCACCAACTA CAGCATCCTC ATTGGGACCA ACAAATTCTA TGAGATCGAC 
ATGAAGCAGT ACACGCTTGA CGAGTTCCTG GACAAGAACG ACCATTCCTT GGCACCTGCT 
GTGTTCGCCT CCTCGTCCAA CAGCTTCCCT GTCTCCATTG TGCAGGCGAA CAGCGCCGGG 
CAGCGAGAAG AATACCTGCT GTGCTTCCAC GAATTTGGGG TGTTCGTGGA TTCTTACGGA 
AGACGTAGCC GCACAGATGA TCTCAAGTGG AGTCGCTTAC CTCTGGCCTT CGCCTACAGA 
GAACCTTATC TGTTTGTGAC TCACTTCAAC TCCCTGGAAG TCATTGAGAT CCAGGCCAGA 
TCCTCACTGG GGAGCCCTGC CCGAGCATAT CTGGAAATTC CAAACCCTCG CTACCTGGGC 
CCCGCGATTT CCTCCGGAGC GATTTACCTG GCCTCCTCAT ACCAGGACAA GTTAAGGGTC 
ATATGCTGCA AAGGAAACCT CGTGAAGGAG TCAGGCACTG AGCAGCACCG GGTGCCCTCC 
ACCTCCCGCA GCAGCCCCAA CAAGCGAGGC CCACCAACAT ACAACGAGCA CATCACCAAA 
CGCGTGGCCT CCAGCCCGGC GCCACCGGAA GGCCCCAGCC ACCCCCGAGA GCCAAGCACA 
CCGCACCGCT ACCGAGACAG AGAGGGTCGG ACAGAGCTGC GCAGGGACAA GTCTCCAGGC 
CGCCCTCTGG AGCGGGAGAA GTCCCCAGGC CGAATGCTCA GCACTAGGAG AGAGCGGTCC 
CCAGGGAGAC TGTTTGAAGA CAGCAGCAGG GGCCGGCTGC CTGCAGGAGC AGTGAGGACC 
CCACTGTCCC AGGTTAACAA GGTCTGGGAC CAGTCTTCAG TATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cit ( XM_006530139.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006530202.1 Mus musculus citron (Cit), transcript variant X10, mRNA.

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CloneID	OMu35780
Clone ID Related Accession (Same CDS sequence)	XM_006530139.3 , XM_006530139.4
Accession Version	XM_006530139.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6246bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	citron Rho-interacting kinase isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000071.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006530139.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTGAAGT TCAAGTATGG TGTGCGGAAC CCGCCGGAGG CCAGTGCCTC CGAGCCCATT 
GCCAGTCGGG CCTCCAGGCT AAATCTCTTC TTCCAGGGGA AACCGCCCCT CATGACTCAA 
CAGCAGATGT CTGCTCTTTC CCGGGAAGGG ATGCTAGACG CCCTCTTCGC TCTCTTTGAA 
GAGTGCAGCC AACCCGCCCT GATGAAGATG AAGCACGTGA GCAGCTTTGT CCAGAAGTAT 
TCCGACACCA TAGCCGAGTT GCGGGAGCTG CAGCCGTCGG CGAGAGACTT CGAAGTTCGA 
AGCCTTGTGG GCTGTGGTCA CTTCGCTGAA GTGCAGGTGG TTAGAGAGAA GGCGACCGGG 
GACGTCTATG CCATGAAAAT CATGAAGAAG AAGGCTTTGC TGGCCCAGGA ACAGGTTTCA 
TTTTTCGAGG AGGAGAGGAA CATATTATCT CGGAGCACGA GTCCTTGGAT CCCCCAGTTA 
CAGTACGCCT TTCAGGACAA AAATAACCTT TACCTGGTCA TGGAATATCA GCCTGGAGGG 
GATTTGCTGT CGCTTCTGAA CAGATACGAG GACCAATTAG ATGAGAGCAT GATCCAGTTT 
TACCTTGCTG AGCTGATTTT GGCTGTCCAC AGCGTGCACC AGATGGGATA TGTGCATCGA 
GACATCAAGC CCGAGAACAT CCTCATCGAC CGGACGGGAC ACATCAAGCT GGTGGATTTT 
GGATCAGCCG CTAAGATGAA TTCAAATAAG GTGGATGCCA AACTCCCCAT TGGGACCCCG 
GATTACATGG CTCCGGAAGT GTTGACCGTG ATGAACGAGG ACCGAAGGGG CACATACGGC 
TTGGACTGTG ACTGGTGGTC TGTCGGAGTT GTTGCTTATG AGATGGTTTA TGGGAAGACC 
CCATTCACAG AGGGAACCTC CGCCCGGACC TTCAACAACA TCATGAACTT CCAGCGGTTT 
TTGAAGTTCC CAGATGACCC CAAAGTTAGC AGTGAGCTCC TTGATCTGCT TCAGAGTCTG 
CTGTGTGTCC AGAAAGAGAG ACTGAAGTTC GAGGGTCTCT GCTGCCACCC TTTCTTTGCC 
AGAACGGACT GGAACAACAT CCGTAACTCT CCTCCCCCCT TCGTCCCCAC CCTCAAGTCT 
GACGATGACA CCTCCAATTT TGATGAACCA GAGAAGAATT CGTGGGTTTC ATCCTCTGTG 
TGCCAGCTGA GCCCCTCGGG CTTCTCAGGC GAAGAGCTGC CGTTTGTGGG ATTTTCGTAC 
AGCAAGGCAC TGGGGTATCT TGGTAGATCT GAGTCTGTCG TGTCGAGTCT GGACTCCCCT 
GCCAAGGTTA GCTCCATGGA AAAGAAACTT CTCATCAAAA GCAAAGAGCT CCAAGACTCC 
CAGGACAAGT GTCACAAGAT GGAGCAGGAA ATGACCCGGT TACATCGCAG AGTGTCAGAG 
GTGGAGGCTG TGCTTAGTCA GAAGGAGGTG GAGCTGAAGG CCTCTGAGAC TCAGAGATCC 
CTCCTGGAGC AGGACCTTGC TACCTACATC ACAGAATGCA GTAGCTTAAA GCGAAGTTTG 
GAGCAAGCGC GGATGGAGGT GTCCCAGGAG GATGACAAAG CTCTGCAGCT TCTCCACGAC 
ATCCGAGAGC AGAGCCGGAA GCTCCAGGAG ATCAAGGAGC AGGAGTACCA GGCTCAGGTG 
GAGGAGATGA GGCTGATGAT GAATCAGCTG GAAGAAGACC TGGTGTCAGC CCGCAGACGC 
AGCGATCTCT ACGAGTCTGA GCTGAGGGAG TCTCGGCTTG CCGCCGAGGA ATTCAAGCGG 
AAGGCAAACG AATGTCAGCA CAAACTGATG AAGGTAGTCA GCCACCCTCC AAGAGGGGAC 
TCCGGGGGCA CGGCCCTGGA TGATCTTCAC AAGATGCAAG GCCACGCTGG CTTAACGAGT 
GCTAAGGACC AAGGGAAGCC TGAAGTGGGA GAATATTCCA AACTGGAGAA GATCAATGCT 
GAGCAGCAGC TGAAGATCCA GGAGCTCCAG GAGAAGCTGG AGAAGGCTGT AAAAGCCAGC 
ACAGAGGCCA CCGAGCTCCT GCAGAATATC CGCCAGGCAA AGGAGCGAGC AGAGCGGGAG 
CTAGAGAAGC TACACAACCG GGAAGACTCC TCCGAGGGCA TCAAAAAGAA GCTGGTGGAA 
GCCGAGGAGC TGGAGGAGAA ACATCGGGAG GCTCAGGTCT CAGCTCAACA TCTAGAAGTA 
CACTTGAAAC AGAAAGAACA GCACTACGAG GAAAAGATCA AGGTATTGGA CAATCAGATA 
AAGAAGGACC TGGCCGACAA GGAGAGCCTG GAGAACATGA TGCAGAGACA CGAGGAGGAG 
GCCCACGAGA AGGGCAAGAT CCTCAGCGAG CAGAAGGCGA TGATCAACGC GATGGATTCC 
AAGATCCGAT CCCTGGAGCA GAGGATCGTG GAGCTGTCGG AAGCCAACAA GCTTGCGGCA 
AACAGCAGTC TCTTCACCCA GAGGAACATG AAGGCCCAGG AAGAGATGAT CTCAGAACTC 
AGGCAGCAGA AATTTTACCT GGAGACGCAG GCCGGAAAGC TGGAGGCCCA GAACCGAAAG 
CTGGAAGAGC AACTGGAGAA GATCAGCCAC CAAGATCACA GTGACAAGAG TCGGCTGCTG 
GAGCTGGAAA CAAGGCTGAG GGAGGTCAGC CTGGAGCACG AGGAGCAGAA GCTGGAGCTG 
AAGCGGCAGC TCACGGAGCT GCAGCTGTCC CTGCAGGAGC GCGAGTCCCA GCTGACGGCC 
CTGCAGGCCG CCCGGGCAGC GCTGGAGAGC CAGCTCCGCC AGGCGAAGAC AGAGCTGGAG 
GAGACAACCG CGGAAGCGGA GGAGGAGATC CAGGCGCTCA CGGCACATCG AGATGAGATC 
CAGCGCAAAT TCGATGCCCT TCGCAACAGC TGCACGGTCA TCACCGACCT GGAGGAGCAG 
CTGAACCAGC TCACCGAGGA CAACGCCGAG CTCAACAACC AAAACTTCTA CCTGTCCAAA 
CAACTCGATG AGGCTTCCGG GGCCAATGAC GAGATTGTGC AGCTGCGGAG TGAGGTGGAC 
CATCTCCGCC GTGAGATCAC GGAGCGGGAG ATGCAGCTCA CCAGCCAGAA GCAAGCACAA 
CTCAGTGCTC CTGATTTGCA GACGATGGAG GCTCTGAAGA CGACATGCAC CATGCTGGAG 
GAGCAGGTCC TGGACCTGGA GGCCCTGAAC GACGAGCTGC TGGAGAAGGA GCGCCAGTGG 
GAGGCCTGGC GGAGCGTCCT CGGCGACGAG AAGTCCCAGT TCGAGTGTCG AGTTCGAGAG 
CTACAGAGGA TGCTGGACAC CGAGAAGCAG AGCAGGGCTA GGGCCGATCA GCGGATCACC 
GAGTCGCGCC AGGTGGTGGA GTTGGCGGTG AAGGAACACA AGGCCGAGAT TCTTGCTCTG 
CAGCAGGCTC TCAAGGAGCA GAAGCTCAAA GCCGAGAGCC TGTCGGACAA GCTCAACGAC 
CTGGAGAAGA AGCACGCCAT GCTGGAGATG AACGCCCGGA GCTTACAGCA GAAACTAGAG 
ACAGAGCGGG AGCTCAAACA GAGGCTTCTG GAGGAGCAAG CCAAATTGCA GCAGCAGATG 
GACCTGCAGA AGAACCACAT CTTCAGACTG ACGCAAGGGC TGCAGGAGGC GCTGGACCGG 
GCGGATCTGC TGAAGACAGA AAGGAGCGAC CTGGAGTACC AGCTGGAAAA CATTCAGGTT 
CTCTACTCTC ACGAGAAAGT GAAAATGGAA GGCACAATCT CTCAGCAAAC CAAACTCATT 
GATTTCCTGC AAGCCAAAAT GGACCAGCCT GCTAAAAAGA AAAAGGGTTT ATTTAGTCGA 
CGGAAAGAGG ACCCTGCTTT GCCCACACAG GTTCCTCTGC AGTACAATGA GCTGAAGCTA 
GCCCTGGAAA AGGAGAAAGC CCGATGCGCG GAGCTGGAGG AGGCCCTTCA GAAGACCCGC 
ATCGAACTCC GCTCTGCGCG GGAGGAAGCT GCCCACCGCA AAGCCACGGA CCACCCGCAC 
CCGTCTACGC CAGCCACTGC GAGGCAGCAG ATCGCCATGT CTGCCATTGT GCGGTCGCCC 
GAGCACCAGC CCAGTGCCAT GAGCCTGCTT GCCCCACCAT CCAGCCGCAG AAAGGAGTCG 
TCAACTCCAG AGGAATTCAG CCGGCGTCTG AAAGAGCGCA TGCACCACAA CATCCCTCAC 
CGGTTTAATG TGGGCCTGAA CATGAGAGCC ACCAAGTGCG CCGTGTGTCT GGATACTGTG 
CACTTTGGAC GCCAGGCATC CAAATGCCTC GAATGTCAAG TGATGTGTCA CCCCAAATGC 
TCCACCTGCT TGCCCGCTAC CTGTGGCCTG CCAGCTGAAT ATGCCACACA CTTCACTGAG 
GCCTTCTGCC GGGACAAAAT GAACTCCCCG GGGCTCCAGA GCAAGGAGCC TGGCAGCAGC 
TTGCACCTGG AAGGGTGGAT GAAAGTGCCC AGAAATAACA AACGGGGACA GCAAGGCTGG 
GACAGGAAGT ACATTGTTCT GGAGGGGTCA AAAGTCCTCA TCTATGACAA TGAAGCCAGA 
GAAGCTGGAC AGAGGCCGGT GGAAGAATTT GAGCTGTGCC TTCCTGACGG GGACGTATCT 
ATTCATGGTG CCGTTGGTGC TTCAGAACTT GCAAATACCG CCAAAGCAGA TGTCCCATAC 
ATCCTGAAGA TGGAGTCTCA CCCACACACC ACCTGCTGGC CTGGGAGAAC CCTCTACTTG 
CTAGCACCCA GCTTCCCCGA CAAGCAGCGC TGGGTCACCG CCTTAGAATC TGTCGTCGCA 
GGTGGGAGAG TTTCTAGGGA AAAGGCCGAA GCCGATGCTG CACGTGACCA TACTTCGAGT 
GAGCATCAGC CAGTGTGGGT TGAGAAATTA CTTGGAAACT CTCTGCTGAA ACTGGAGGGC 
GATGACCGGC TTGACATGAA CTGCACCCTG CCCTTCAGTG ACCAGGTAGT GCTGGTGGGC 
ACGGAGGAAG GCCTCTACGC CCTGAATGTC TTGAAAAACT CCTTAACCCA CATCCCAGGG 
ATTGGCGCAG TCTTCCAAAT TTACATCATC AAGGACCTGG AGAAGCTGCT CATGATAGCA 
GGGGAAGAGC GGGCTCTGTG CCTGGTGGAC GTGAAGAAGG TGAAGCAGTC CCTGGCGCAG 
TCACACCTGC CTGCCCAGCC CGACGTCTCC CCCAACATAT TCGAAGCCGT CAAAGGCTGC 
CACTTGTTCG CTGCTGGCAA GATCGAGAAC AGCCTGTGCA TCTGCGCCGC TATGCCAAGC 
AAAGTCGTCA TCCTCCGCTA CAATGACAAC CTCAGCAAGT ACTGCATCCG CAAGGAGATC 
GAGACCTCAG AGCCCTGCAG CTGTATCCAC TTCACCAACT ACAGCATCCT CATTGGGACC 
AACAAATTCT ATGAGATCGA CATGAAGCAG TACACGCTTG ACGAGTTCCT GGACAAGAAC 
GACCATTCCT TGGCACCTGC TGTGTTCGCC TCCTCGTCCA ACAGCTTCCC TGTCTCCATT 
GTGCAGGCGA ACAGCGCCGG GCAGCGAGAA GAATACCTGC TGTGCTTCCA CGAATTTGGG 
GTGTTCGTGG ATTCTTACGG AAGACGTAGC CGCACAGATG ATCTCAAGTG GAGTCGCTTA 
CCTCTGGCCT TCGCCTACAG AGAACCTTAT CTGTTTGTGA CTCACTTCAA CTCCCTGGAA 
GTCATTGAGA TCCAGGCCAG ATCCTCACTG GGGAGCCCTG CCCGAGCATA TCTGGAAATT 
CCAAACCCTC GCTACCTGGG CCCCGCGATT TCCTCCGGAG CGATTTACCT GGCCTCCTCA 
TACCAGGACA AGTTAAGGGT CATATGCTGC AAAGGAAACC TCGTGAAGGA GTCAGGCACT 
GAGCAGCACC GGGTGCCCTC CACCTCCCGC AGCAGCCCCA ACAAGCGAGG CCCACCAACA 
TACAACGAGC ACATCACCAA ACGCGTGGCC TCCAGCCCGG CGCCACCGGA AGGCCCCAGC 
CACCCCCGAG AGCCAAGCAC ACCGCACCGC TACCGAGACA GAGAGGGTCG GACAGAGCTG 
CGCAGGGACA AGTCTCCAGG CCGCCCTCTG GAGCGGGAGA AGTCCCCAGG CCGAATGCTC 
AGCACTAGGA GAGAGCGGTC CCCAGGGAGA CTGTTTGAAG ACAGCAGCAG GGGCCGGCTG 
CCTGCAGGAG CAGTGAGGAC CCCACTGTCC CAGGTTAACA AGGTCTGGGA CCAGTCTTCA 
GTATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006530202.1
CDS	158..6403
Translation	MLKFKYGVRNPPEASASEPIASRASRLNLFFQGKPPLMTQQQMSALSREGMLDALFALFEECSQPALMKMKHVSSFVQKYSDTIAELRELQPSARDFEVRSLVGCGHFAEVQVVREKATGDVYAMKIMKKKALLAQEQVSFFEEERNILSRSTSPWIPQLQYAFQDKNNLYLVMEYQPGGDLLSLLNRYEDQLDESMIQFYLAELILAVHSVHQMGYVHRDIKPENILIDRTGHIKLVDFGSAAKMNSNKVDAKLPIGTPDYMAPEVLTVMNEDRRGTYGLDCDWWSVGVVAYEMVYGKTPFTEGTSARTFNNIMNFQRFLKFPDDPKVSSELLDLLQSLLCVQKERLKFEGLCCHPFFARTDWNNIRNSPPPFVPTLKSDDDTSNFDEPEKNSWVSSSVCQLSPSGFSGEELPFVGFSYSKALGYLGRSESVVSSLDSPAKVSSMEKKLLIKSKELQDSQDKCHKMEQEMTRLHRRVSEVEAVLSQKEVELKASETQRSLLEQDLATYITECSSLKRSLEQARMEVSQEDDKALQLLHDIREQSRKLQEIKEQEYQAQVEEMRLMMNQLEEDLVSARRRSDLYESELRESRLAAEEFKRKANECQHKLMKVVSHPPRGDSGGTALDDLHKMQGHAGLTSAKDQGKPEVGEYSKLEKINAEQQLKIQELQEKLEKAVKASTEATELLQNIRQAKERAERELEKLHNREDSSEGIKKKLVEAEELEEKHREAQVSAQHLEVHLKQKEQHYEEKIKVLDNQIKKDLADKESLENMMQRHEEEAHEKGKILSEQKAMINAMDSKIRSLEQRIVELSEANKLAANSSLFTQRNMKAQEEMISELRQQKFYLETQAGKLEAQNRKLEEQLEKISHQDHSDKSRLLELETRLREVSLEHEEQKLELKRQLTELQLSLQERESQLTALQAARAALESQLRQAKTELEETTAEAEEEIQALTAHRDEIQRKFDALRNSCTVITDLEEQLNQLTEDNAELNNQNFYLSKQLDEASGANDEIVQLRSEVDHLRREITEREMQLTSQKQAQLSAPDLQTMEALKTTCTMLEEQVLDLEALNDELLEKERQWEAWRSVLGDEKSQFECRVRELQRMLDTEKQSRARADQRITESRQVVELAVKEHKAEILALQQALKEQKLKAESLSDKLNDLEKKHAMLEMNARSLQQKLETERELKQRLLEEQAKLQQQMDLQKNHIFRLTQGLQEALDRADLLKTERSDLEYQLENIQVLYSHEKVKMEGTISQQTKLIDFLQAKMDQPAKKKKGLFSRRKEDPALPTQVPLQYNELKLALEKEKARCAELEEALQKTRIELRSAREEAAHRKATDHPHPSTPATARQQIAMSAIVRSPEHQPSAMSLLAPPSSRRKESSTPEEFSRRLKERMHHNIPHRFNVGLNMRATKCAVCLDTVHFGRQASKCLECQVMCHPKCSTCLPATCGLPAEYATHFTEAFCRDKMNSPGLQSKEPGSSLHLEGWMKVPRNNKRGQQGWDRKYIVLEGSKVLIYDNEAREAGQRPVEEFELCLPDGDVSIHGAVGASELANTAKADVPYILKMESHPHTTCWPGRTLYLLAPSFPDKQRWVTALESVVAGGRVSREKAEADAARDHTSSEHQPVWVEKLLGNSLLKLEGDDRLDMNCTLPFSDQVVLVGTEEGLYALNVLKNSLTHIPGIGAVFQIYIIKDLEKLLMIAGEERALCLVDVKKVKQSLAQSHLPAQPDVSPNIFEAVKGCHLFAAGKIENSLCICAAMPSKVVILRYNDNLSKYCIRKEIETSEPCSCIHFTNYSILIGTNKFYEIDMKQYTLDEFLDKNDHSLAPAVFASSSNSFPVSIVQANSAGQREEYLLCFHEFGVFVDSYGRRSRTDDLKWSRLPLAFAYREPYLFVTHFNSLEVIEIQARSSLGSPARAYLEIPNPRYLGPAISSGAIYLASSYQDKLRVICCKGNLVKESGTEQHRVPSTSRSSPNKRGPPTYNEHITKRVASSPAPPEGPSHPREPSTPHRYRDREGRTELRRDKSPGRPLEREKSPGRMLSTRRERSPGRLFEDSSRGRLPAGAVRTPLSQVNKVWDQSSV

Target ORF information:

RefSeq Version	XM_006530139.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus citron (Cit), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006530139.3

ORF Insert Sequence:

ATGTTGAAGT TCAAGTATGG TGTGCGGAAC CCGCCGGAGG CCAGTGCCTC CGAGCCCATT 
GCCAGTCGGG CCTCCAGGCT AAATCTCTTC TTCCAGGGGA AACCGCCCCT CATGACTCAA 
CAGCAGATGT CTGCTCTTTC CCGGGAAGGG ATGCTAGACG CCCTCTTCGC TCTCTTTGAA 
GAGTGCAGCC AACCCGCCCT GATGAAGATG AAGCACGTGA GCAGCTTTGT CCAGAAGTAT 
TCCGACACCA TAGCCGAGTT GCGGGAGCTG CAGCCGTCGG CGAGAGACTT CGAAGTTCGA 
AGCCTTGTGG GCTGTGGTCA CTTCGCTGAA GTGCAGGTGG TTAGAGAGAA GGCGACCGGG 
GACGTCTATG CCATGAAAAT CATGAAGAAG AAGGCTTTGC TGGCCCAGGA ACAGGTTTCA 
TTTTTCGAGG AGGAGAGGAA CATATTATCT CGGAGCACGA GTCCTTGGAT CCCCCAGTTA 
CAGTACGCCT TTCAGGACAA AAATAACCTT TACCTGGTCA TGGAATATCA GCCTGGAGGG 
GATTTGCTGT CGCTTCTGAA CAGATACGAG GACCAATTAG ATGAGAGCAT GATCCAGTTT 
TACCTTGCTG AGCTGATTTT GGCTGTCCAC AGCGTGCACC AGATGGGATA TGTGCATCGA 
GACATCAAGC CCGAGAACAT CCTCATCGAC CGGACGGGAC ACATCAAGCT GGTGGATTTT 
GGATCAGCCG CTAAGATGAA TTCAAATAAG GTGGATGCCA AACTCCCCAT TGGGACCCCG 
GATTACATGG CTCCGGAAGT GTTGACCGTG ATGAACGAGG ACCGAAGGGG CACATACGGC 
TTGGACTGTG ACTGGTGGTC TGTCGGAGTT GTTGCTTATG AGATGGTTTA TGGGAAGACC 
CCATTCACAG AGGGAACCTC CGCCCGGACC TTCAACAACA TCATGAACTT CCAGCGGTTT 
TTGAAGTTCC CAGATGACCC CAAAGTTAGC AGTGAGCTCC TTGATCTGCT TCAGAGTCTG 
CTGTGTGTCC AGAAAGAGAG ACTGAAGTTC GAGGGTCTCT GCTGCCACCC TTTCTTTGCC 
AGAACGGACT GGAACAACAT CCGTAACTCT CCTCCCCCCT TCGTCCCCAC CCTCAAGTCT 
GACGATGACA CCTCCAATTT TGATGAACCA GAGAAGAATT CGTGGGTTTC ATCCTCTGTG 
TGCCAGCTGA GCCCCTCGGG CTTCTCAGGC GAAGAGCTGC CGTTTGTGGG ATTTTCGTAC 
AGCAAGGCAC TGGGGTATCT TGGTAGATCT GAGTCTGTCG TGTCGAGTCT GGACTCCCCT 
GCCAAGGTTA GCTCCATGGA AAAGAAACTT CTCATCAAAA GCAAAGAGCT CCAAGACTCC 
CAGGACAAGT GTCACAAGAT GGAGCAGGAA ATGACCCGGT TACATCGCAG AGTGTCAGAG 
GTGGAGGCTG TGCTTAGTCA GAAGGAGGTG GAGCTGAAGG CCTCTGAGAC TCAGAGATCC 
CTCCTGGAGC AGGACCTTGC TACCTACATC ACAGAATGCA GTAGCTTAAA GCGAAGTTTG 
GAGCAAGCGC GGATGGAGGT GTCCCAGGAG GATGACAAAG CTCTGCAGCT TCTCCACGAC 
ATCCGAGAGC AGAGCCGGAA GCTCCAGGAG ATCAAGGAGC AGGAGTACCA GGCTCAGGTG 
GAGGAGATGA GGCTGATGAT GAATCAGCTG GAAGAAGACC TGGTGTCAGC CCGCAGACGC 
AGCGATCTCT ACGAGTCTGA GCTGAGGGAG TCTCGGCTTG CCGCCGAGGA ATTCAAGCGG 
AAGGCAAACG AATGTCAGCA CAAACTGATG AAGGTAGTCA GCCACCCTCC AAGAGGGGAC 
TCCGGGGGCA CGGCCCTGGA TGATCTTCAC AAGATGCAAG GCCACGCTGG CTTAACGAGT 
GCTAAGGACC AAGGGAAGCC TGAAGTGGGA GAATATTCCA AACTGGAGAA GATCAATGCT 
GAGCAGCAGC TGAAGATCCA GGAGCTCCAG GAGAAGCTGG AGAAGGCTGT AAAAGCCAGC 
ACAGAGGCCA CCGAGCTCCT GCAGAATATC CGCCAGGCAA AGGAGCGAGC AGAGCGGGAG 
CTAGAGAAGC TACACAACCG GGAAGACTCC TCCGAGGGCA TCAAAAAGAA GCTGGTGGAA 
GCCGAGGAGC TGGAGGAGAA ACATCGGGAG GCTCAGGTCT CAGCTCAACA TCTAGAAGTA 
CACTTGAAAC AGAAAGAACA GCACTACGAG GAAAAGATCA AGGTATTGGA CAATCAGATA 
AAGAAGGACC TGGCCGACAA GGAGAGCCTG GAGAACATGA TGCAGAGACA CGAGGAGGAG 
GCCCACGAGA AGGGCAAGAT CCTCAGCGAG CAGAAGGCGA TGATCAACGC GATGGATTCC 
AAGATCCGAT CCCTGGAGCA GAGGATCGTG GAGCTGTCGG AAGCCAACAA GCTTGCGGCA 
AACAGCAGTC TCTTCACCCA GAGGAACATG AAGGCCCAGG AAGAGATGAT CTCAGAACTC 
AGGCAGCAGA AATTTTACCT GGAGACGCAG GCCGGAAAGC TGGAGGCCCA GAACCGAAAG 
CTGGAAGAGC AACTGGAGAA GATCAGCCAC CAAGATCACA GTGACAAGAG TCGGCTGCTG 
GAGCTGGAAA CAAGGCTGAG GGAGGTCAGC CTGGAGCACG AGGAGCAGAA GCTGGAGCTG 
AAGCGGCAGC TCACGGAGCT GCAGCTGTCC CTGCAGGAGC GCGAGTCCCA GCTGACGGCC 
CTGCAGGCCG CCCGGGCAGC GCTGGAGAGC CAGCTCCGCC AGGCGAAGAC AGAGCTGGAG 
GAGACAACCG CGGAAGCGGA GGAGGAGATC CAGGCGCTCA CGGCACATCG AGATGAGATC 
CAGCGCAAAT TCGATGCCCT TCGCAACAGC TGCACGGTCA TCACCGACCT GGAGGAGCAG 
CTGAACCAGC TCACCGAGGA CAACGCCGAG CTCAACAACC AAAACTTCTA CCTGTCCAAA 
CAACTCGATG AGGCTTCCGG GGCCAATGAC GAGATTGTGC AGCTGCGGAG TGAGGTGGAC 
CATCTCCGCC GTGAGATCAC GGAGCGGGAG ATGCAGCTCA CCAGCCAGAA GCAAGCACAA 
CTCAGTGCTC CTGATTTGCA GACGATGGAG GCTCTGAAGA CGACATGCAC CATGCTGGAG 
GAGCAGGTCC TGGACCTGGA GGCCCTGAAC GACGAGCTGC TGGAGAAGGA GCGCCAGTGG 
GAGGCCTGGC GGAGCGTCCT CGGCGACGAG AAGTCCCAGT TCGAGTGTCG AGTTCGAGAG 
CTACAGAGGA TGCTGGACAC CGAGAAGCAG AGCAGGGCTA GGGCCGATCA GCGGATCACC 
GAGTCGCGCC AGGTGGTGGA GTTGGCGGTG AAGGAACACA AGGCCGAGAT TCTTGCTCTG 
CAGCAGGCTC TCAAGGAGCA GAAGCTCAAA GCCGAGAGCC TGTCGGACAA GCTCAACGAC 
CTGGAGAAGA AGCACGCCAT GCTGGAGATG AACGCCCGGA GCTTACAGCA GAAACTAGAG 
ACAGAGCGGG AGCTCAAACA GAGGCTTCTG GAGGAGCAAG CCAAATTGCA GCAGCAGATG 
GACCTGCAGA AGAACCACAT CTTCAGACTG ACGCAAGGGC TGCAGGAGGC GCTGGACCGG 
GCGGATCTGC TGAAGACAGA AAGGAGCGAC CTGGAGTACC AGCTGGAAAA CATTCAGGTT 
CTCTACTCTC ACGAGAAAGT GAAAATGGAA GGCACAATCT CTCAGCAAAC CAAACTCATT 
GATTTCCTGC AAGCCAAAAT GGACCAGCCT GCTAAAAAGA AAAAGGGTTT ATTTAGTCGA 
CGGAAAGAGG ACCCTGCTTT GCCCACACAG GTTCCTCTGC AGTACAATGA GCTGAAGCTA 
GCCCTGGAAA AGGAGAAAGC CCGATGCGCG GAGCTGGAGG AGGCCCTTCA GAAGACCCGC 
ATCGAACTCC GCTCTGCGCG GGAGGAAGCT GCCCACCGCA AAGCCACGGA CCACCCGCAC 
CCGTCTACGC CAGCCACTGC GAGGCAGCAG ATCGCCATGT CTGCCATTGT GCGGTCGCCC 
GAGCACCAGC CCAGTGCCAT GAGCCTGCTT GCCCCACCAT CCAGCCGCAG AAAGGAGTCG 
TCAACTCCAG AGGAATTCAG CCGGCGTCTG AAAGAGCGCA TGCACCACAA CATCCCTCAC 
CGGTTTAATG TGGGCCTGAA CATGAGAGCC ACCAAGTGCG CCGTGTGTCT GGATACTGTG 
CACTTTGGAC GCCAGGCATC CAAATGCCTC GAATGTCAAG TGATGTGTCA CCCCAAATGC 
TCCACCTGCT TGCCCGCTAC CTGTGGCCTG CCAGCTGAAT ATGCCACACA CTTCACTGAG 
GCCTTCTGCC GGGACAAAAT GAACTCCCCG GGGCTCCAGA GCAAGGAGCC TGGCAGCAGC 
TTGCACCTGG AAGGGTGGAT GAAAGTGCCC AGAAATAACA AACGGGGACA GCAAGGCTGG 
GACAGGAAGT ACATTGTTCT GGAGGGGTCA AAAGTCCTCA TCTATGACAA TGAAGCCAGA 
GAAGCTGGAC AGAGGCCGGT GGAAGAATTT GAGCTGTGCC TTCCTGACGG GGACGTATCT 
ATTCATGGTG CCGTTGGTGC TTCAGAACTT GCAAATACCG CCAAAGCAGA TGTCCCATAC 
ATCCTGAAGA TGGAGTCTCA CCCACACACC ACCTGCTGGC CTGGGAGAAC CCTCTACTTG 
CTAGCACCCA GCTTCCCCGA CAAGCAGCGC TGGGTCACCG CCTTAGAATC TGTCGTCGCA 
GGTGGGAGAG TTTCTAGGGA AAAGGCCGAA GCCGATGCTG CACGTGACCA TACTTCGAGT 
GAGCATCAGC CAGTGTGGGT TGAGAAATTA CTTGGAAACT CTCTGCTGAA ACTGGAGGGC 
GATGACCGGC TTGACATGAA CTGCACCCTG CCCTTCAGTG ACCAGGTAGT GCTGGTGGGC 
ACGGAGGAAG GCCTCTACGC CCTGAATGTC TTGAAAAACT CCTTAACCCA CATCCCAGGG 
ATTGGCGCAG TCTTCCAAAT TTACATCATC AAGGACCTGG AGAAGCTGCT CATGATAGCA 
GGGGAAGAGC GGGCTCTGTG CCTGGTGGAC GTGAAGAAGG TGAAGCAGTC CCTGGCGCAG 
TCACACCTGC CTGCCCAGCC CGACGTCTCC CCCAACATAT TCGAAGCCGT CAAAGGCTGC 
CACTTGTTCG CTGCTGGCAA GATCGAGAAC AGCCTGTGCA TCTGCGCCGC TATGCCAAGC 
AAAGTCGTCA TCCTCCGCTA CAATGACAAC CTCAGCAAGT ACTGCATCCG CAAGGAGATC 
GAGACCTCAG AGCCCTGCAG CTGTATCCAC TTCACCAACT ACAGCATCCT CATTGGGACC 
AACAAATTCT ATGAGATCGA CATGAAGCAG TACACGCTTG ACGAGTTCCT GGACAAGAAC 
GACCATTCCT TGGCACCTGC TGTGTTCGCC TCCTCGTCCA ACAGCTTCCC TGTCTCCATT 
GTGCAGGCGA ACAGCGCCGG GCAGCGAGAA GAATACCTGC TGTGCTTCCA CGAATTTGGG 
GTGTTCGTGG ATTCTTACGG AAGACGTAGC CGCACAGATG ATCTCAAGTG GAGTCGCTTA 
CCTCTGGCCT TCGCCTACAG AGAACCTTAT CTGTTTGTGA CTCACTTCAA CTCCCTGGAA 
GTCATTGAGA TCCAGGCCAG ATCCTCACTG GGGAGCCCTG CCCGAGCATA TCTGGAAATT 
CCAAACCCTC GCTACCTGGG CCCCGCGATT TCCTCCGGAG CGATTTACCT GGCCTCCTCA 
TACCAGGACA AGTTAAGGGT CATATGCTGC AAAGGAAACC TCGTGAAGGA GTCAGGCACT 
GAGCAGCACC GGGTGCCCTC CACCTCCCGC AGCAGCCCCA ACAAGCGAGG CCCACCAACA 
TACAACGAGC ACATCACCAA ACGCGTGGCC TCCAGCCCGG CGCCACCGGA AGGCCCCAGC 
CACCCCCGAG AGCCAAGCAC ACCGCACCGC TACCGAGACA GAGAGGGTCG GACAGAGCTG 
CGCAGGGACA AGTCTCCAGG CCGCCCTCTG GAGCGGGAGA AGTCCCCAGG CCGAATGCTC 
AGCACTAGGA GAGAGCGGTC CCCAGGGAGA CTGTTTGAAG ACAGCAGCAG GGGCCGGCTG 
CCTGCAGGAG CAGTGAGGAC CCCACTGTCC CAGGTTAACA AGGTCTGGGA CCAGTCTTCA 
GTATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cit ( XM_006530140.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006530203.1 Mus musculus citron (Cit), transcript variant X11, mRNA.

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CloneID	OMu35781
Clone ID Related Accession (Same CDS sequence)	XM_006530140.4 , XM_006530140.3
Accession Version	XM_006530140.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6213bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	citron Rho-interacting kinase isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000071.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006530140.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTGAAGT TCAAGTATGG TGTGCGGAAC CCGCCGGAGG CCAGTGCCTC CGAGCCCATT 
GCCAGTCGGG CCTCCAGGCT AAATCTCTTC TTCCAGGGGA AACCGCCCCT CATGACTCAA 
CAGCAGATGT CTGCTCTTTC CCGGGAAGGG ATGCTAGACG CCCTCTTCGC TCTCTTTGAA 
GAGTGCAGCC AACCCGCCCT GATGAAGATG AAGCACGTGA GCAGCTTTGT CCAGAAGTAT 
TCCGACACCA TAGCCGAGTT GCGGGAGCTG CAGCCGTCGG CGAGAGACTT CGAAGTTCGA 
AGCCTTGTGG GCTGTGGTCA CTTCGCTGAA GTGCAGGTGG TTAGAGAGAA GGCGACCGGG 
GACGTCTATG CCATGAAAAT CATGAAGAAG AAGGCTTTGC TGGCCCAGGA ACAGGTTTCA 
TTTTTCGAGG AGGAGAGGAA CATATTATCT CGGAGCACGA GTCCTTGGAT CCCCCAGTTA 
CAGTACGCCT TTCAGGACAA AAATAACCTT TACCTGGTCA TGGAATATCA GCCTGGAGGG 
GATTTGCTGT CGCTTCTGAA CAGATACGAG GACCAATTAG ATGAGAGCAT GATCCAGTTT 
TACCTTGCTG AGCTGATTTT GGCTGTCCAC AGCGTGCACC AGATGGGATA TGTGCATCGA 
GACATCAAGC CCGAGAACAT CCTCATCGAC CGGACGGGAC ACATCAAGCT GGTGGATTTT 
GGATCAGCCG CTAAGATGAA TTCAAATAAG GTGGATGCCA AACTCCCCAT TGGGACCCCG 
GATTACATGG CTCCGGAAGT GTTGACCGTG ATGAACGAGG ACCGAAGGGG CACATACGGC 
TTGGACTGTG ACTGGTGGTC TGTCGGAGTT GTTGCTTATG AGATGGTTTA TGGGAAGACC 
CCATTCACAG AGGGAACCTC CGCCCGGACC TTCAACAACA TCATGAACTT CCAGCGGTTT 
TTGAAGTTCC CAGATGACCC CAAAGTTAGC AGTGAGCTCC TTGATCTGCT TCAGAGTCTG 
CTGTGTGTCC AGAAAGAGAG ACTGAAGTTC GAGGGTCTCT GCTGCCACCC TTTCTTTGCC 
AGAACGGACT GGAACAACAT CCGTAACTCT CCTCCCCCCT TCGTCCCCAC CCTCAAGTCT 
GACGATGACA CCTCCAATTT TGATGAACCA GAGAAGAATT CGTGGGTTTC ATCCTCTGTG 
TGCCAGCTGA GCCCCTCGGG CTTCTCAGGC GAAGAGCTGC CGTTTGTGGG ATTTTCGTAC 
AGCAAGGCAC TGGGGTATCT TGGTAGATCT GAGTCTGTCG TGTCGAGTCT GGACTCCCCT 
GCCAAGGTTA GCTCCATGGA AAAGAAACTT CTCATCAAAA GCAAAGAGCT CCAAGACTCC 
CAGGACAAGT GTCACAAGAT GGAGCAGGAA ATGACCCGGT TACATCGCAG AGTGTCAGAG 
GTGGAGGCTG TGCTTAGTCA GAAGGAGGTG GAGCTGAAGG CCTCTGAGAC TCAGAGATCC 
CTCCTGGAGC AGGACCTTGC TACCTACATC ACAGAATGCA GTAGCTTAAA GCGAAGTTTG 
GAGCAAGCGC GGATGGAGGT GTCCCAGGAG GATGACAAAG CTCTGCAGCT TCTCCACGAC 
ATCCGAGAGC AGAGCCGGAA GCTCCAGGAG ATCAAGGAGC AGGAGTACCA GGCTCAGGTG 
GAGGAGATGA GGCTGATGAT GAATCAGCTG GAAGAAGACC TGGTGTCAGC CCGCAGACGC 
AGCGATCTCT ACGAGTCTGA GCTGAGGGAG TCTCGGCTTG CCGCCGAGGA ATTCAAGCGG 
AAGGCAAACG AATGTCAGCA CAAACTGATG AAGGCTAAGG ACCAAGGGAA GCCTGAAGTG 
GGAGAATATT CCAAACTGGA GAAGATCAAT GCTGAGCAGC AGCTGAAGAT CCAGGAGCTC 
CAGGAGAAGC TGGAGAAGGC TGTAAAAGCC AGCACAGAGG CCACCGAGCT CCTGCAGAAT 
ATCCGCCAGG CAAAGGAGCG AGCAGAGCGG GAGCTAGAGA AGCTACACAA CCGGGAAGAC 
TCCTCCGAGG GCATCAAAAA GAAGCTGGTG GAAGCCGAGG AACGCCGCCA CTCCCTGGAG 
AACAAGGTAA AGAGACTAGA GACCATGGAG CGTAGAGAGA ACAGACTGAA GGATGACATC 
CAGACAAAGT CCGAACAGAT CCAGCAGATG GCTGATAAAA TTCTGGAGCT GGAGGAGAAA 
CATCGGGAGG CTCAGGTCTC AGCTCAACAT CTAGAAGTAC ACTTGAAACA GAAAGAACAG 
CACTACGAGG AAAAGATCAA GGTATTGGAC AATCAGATAA AGAAGGACCT GGCCGACAAG 
GAGAGCCTGG AGAACATGAT GCAGAGACAC GAGGAGGAGG CCCACGAGAA GGGCAAGATC 
CTCAGCGAGC AGAAGGCGAT GATCAACGCG ATGGATTCCA AGATCCGATC CCTGGAGCAG 
AGGATCGTGG AGCTGTCGGA AGCCAACAAG CTTGCGGCAA ACAGCAGTCT CTTCACCCAG 
AGGAACATGA AGGCCCAGGA AGAGATGATC TCAGAACTCA GGCAGCAGAA ATTTTACCTG 
GAGACGCAGG CCGGAAAGCT GGAGGCCCAG AACCGAAAGC TGGAAGAGCA ACTGGAGAAG 
ATCAGCCACC AAGATCACAG TGACAAGAGT CGGCTGCTGG AGCTGGAAAC AAGGCTGAGG 
GAGGTCAGCC TGGAGCACGA GGAGCAGAAG CTGGAGCTGA AGCGGCAGCT CACGGAGCTG 
CAGCTGTCCC TGCAGGAGCG CGAGTCCCAG CTGACGGCCC TGCAGGCCGC CCGGGCAGCG 
CTGGAGAGCC AGCTCCGCCA GGCGAAGACA GAGCTGGAGG AGACAACCGC GGAAGCGGAG 
GAGGAGATCC AGGCGCTCAC GGCACATCGA GATGAGATCC AGCGCAAATT CGATGCCCTT 
CGCAACAGCT GCACGGTCAT CACCGACCTG GAGGAGCAGC TGAACCAGCT CACCGAGGAC 
AACGCCGAGC TCAACAACCA AAACTTCTAC CTGTCCAAAC AACTCGATGA GGCTTCCGGG 
GCCAATGACG AGATTGTGCA GCTGCGGAGT GAGGTGGACC ATCTCCGCCG TGAGATCACG 
GAGCGGGAGA TGCAGCTCAC CAGCCAGAAG CAAACGATGG AGGCTCTGAA GACGACATGC 
ACCATGCTGG AGGAGCAGGT CCTGGACCTG GAGGCCCTGA ACGACGAGCT GCTGGAGAAG 
GAGCGCCAGT GGGAGGCCTG GCGGAGCGTC CTCGGCGACG AGAAGTCCCA GTTCGAGTGT 
CGAGTTCGAG AGCTACAGAG GATGCTGGAC ACCGAGAAGC AGAGCAGGGC TAGGGCCGAT 
CAGCGGATCA CCGAGTCGCG CCAGGTGGTG GAGTTGGCGG TGAAGGAACA CAAGGCCGAG 
ATTCTTGCTC TGCAGCAGGC TCTCAAGGAG CAGAAGCTCA AAGCCGAGAG CCTGTCGGAC 
AAGCTCAACG ACCTGGAGAA GAAGCACGCC ATGCTGGAGA TGAACGCCCG GAGCTTACAG 
CAGAAACTAG AGACAGAGCG GGAGCTCAAA CAGAGGCTTC TGGAGGAGCA AGCCAAATTG 
CAGCAGCAGA TGGACCTGCA GAAGAACCAC ATCTTCAGAC TGACGCAAGG GCTGCAGGAG 
GCGCTGGACC GGGCGGATCT GCTGAAGACA GAAAGGAGCG ACCTGGAGTA CCAGCTGGAA 
AACATTCAGG TTCTCTACTC TCACGAGAAA GTGAAAATGG AAGGCACAAT CTCTCAGCAA 
ACCAAACTCA TTGATTTCCT GCAAGCCAAA ATGGACCAGC CTGCTAAAAA GAAAAAGGGT 
TTATTTAGTC GACGGAAAGA GGACCCTGCT TTGCCCACAC AGGTTCCTCT GCAGTACAAT 
GAGCTGAAGC TAGCCCTGGA AAAGGAGAAA GCCCGATGCG CGGAGCTGGA GGAGGCCCTT 
CAGAAGACCC GCATCGAACT CCGCTCTGCG CGGGAGGAAG CTGCCCACCG CAAAGCCACG 
GACCACCCGC ACCCGTCTAC GCCAGCCACT GCGAGGCAGC AGATCGCCAT GTCTGCCATT 
GTGCGGTCGC CCGAGCACCA GCCCAGTGCC ATGAGCCTGC TTGCCCCACC ATCCAGCCGC 
AGAAAGGAGT CGTCAACTCC AGAGGAATTC AGCCGGCGTC TGAAAGAGCG CATGCACCAC 
AACATCCCTC ACCGGTTTAA TGTGGGCCTG AACATGAGAG CCACCAAGTG CGCCGTGTGT 
CTGGATACTG TGCACTTTGG ACGCCAGGCA TCCAAATGCC TCGAATGTCA AGTGATGTGT 
CACCCCAAAT GCTCCACCTG CTTGCCCGCT ACCTGTGGCC TGCCAGCTGA ATATGCCACA 
CACTTCACTG AGGCCTTCTG CCGGGACAAA ATGAACTCCC CGGGGCTCCA GAGCAAGGAG 
CCTGGCAGCA GCTTGCACCT GGAAGGGTGG ATGAAAGTGC CCAGAAATAA CAAACGGGGA 
CAGCAAGGCT GGGACAGGAA GTACATTGTT CTGGAGGGGT CAAAAGTCCT CATCTATGAC 
AATGAAGCCA GAGAAGCTGG ACAGAGGCCG GTGGAAGAAT TTGAGCTGTG CCTTCCTGAC 
GGGGACGTAT CTATTCATGG TGCCGTTGGT GCTTCAGAAC TTGCAAATAC CGCCAAAGCA 
GATGTCCCAT ACATCCTGAA GATGGAGTCT CACCCACACA CCACCTGCTG GCCTGGGAGA 
ACCCTCTACT TGCTAGCACC CAGCTTCCCC GACAAGCAGC GCTGGGTCAC CGCCTTAGAA 
TCTGTCGTCG CAGGTGGGAG AGTTTCTAGG GAAAAGGCCG AAGCCGATGC TAAATTACTT 
GGAAACTCTC TGCTGAAACT GGAGGGCGAT GACCGGCTTG ACATGAACTG CACCCTGCCC 
TTCAGTGACC AGGTAGTGCT GGTGGGCACG GAGGAAGGCC TCTACGCCCT GAATGTCTTG 
AAAAACTCCT TAACCCACAT CCCAGGGATT GGCGCAGTCT TCCAAATTTA CATCATCAAG 
GACCTGGAGA AGCTGCTCAT GATAGCAGGG GAAGAGCGGG CTCTGTGCCT GGTGGACGTG 
AAGAAGGTGA AGCAGTCCCT GGCGCAGTCA CACCTGCCTG CCCAGCCCGA CGTCTCCCCC 
AACATATTCG AAGCCGTCAA AGGCTGCCAC TTGTTCGCTG CTGGCAAGAT CGAGAACAGC 
CTGTGCATCT GCGCCGCTAT GCCAAGCAAA GTCGTCATCC TCCGCTACAA TGACAACCTC 
AGCAAGTACT GCATCCGCAA GGAGATCGAG ACCTCAGAGC CCTGCAGCTG TATCCACTTC 
ACCAACTACA GCATCCTCAT TGGGACCAAC AAATTCTATG AGATCGACAT GAAGCAGTAC 
ACGCTTGACG AGTTCCTGGA CAAGAACGAC CATTCCTTGG CACCTGCTGT GTTCGCCTCC 
TCGTCCAACA GCTTCCCTGT CTCCATTGTG CAGGCGAACA GCGCCGGGCA GCGAGAAGAA 
TACCTGCTGT GCTTCCACGA ATTTGGGGTG TTCGTGGATT CTTACGGAAG ACGTAGCCGC 
ACAGATGATC TCAAGTGGAG TCGCTTACCT CTGGCCTTCG CCTACAGAGA ACCTTATCTG 
TTTGTGACTC ACTTCAACTC CCTGGAAGTC ATTGAGATCC AGGCCAGATC CTCACTGGGG 
AGCCCTGCCC GAGCATATCT GGAAATTCCA AACCCTCGCT ACCTGGGCCC CGCGATTTCC 
TCCGGAGCGA TTTACCTGGC CTCCTCATAC CAGGACAAGT TAAGGGTCAT ATGCTGCAAA 
GGAAACCTCG TGAAGGAGTC AGGCACTGAG CAGCACCGGG TGCCCTCCAC CTCCCGCAGC 
AGCCCCAACA AGCGAGGCCC ACCAACATAC AACGAGCACA TCACCAAACG CGTGGCCTCC 
AGCCCGGCGC CACCGGAAGG CCCCAGCCAC CCCCGAGAGC CAAGCACACC GCACCGCTAC 
CGAGACAGAG AGGGTCGGAC AGAGCTGCGC AGGGACAAGT CTCCAGGCCG CCCTCTGGAG 
CGGGAGAAGT CCCCAGGCCG AATGCTCAGC ACTAGGAGAG AGCGGTCCCC AGGGAGACTG 
TTTGAAGACA GCAGCAGGGG CCGGCTGCCT GCAGGAGCAG TGAGGACCCC ACTGTCCCAG 
GTTAACAAGG TCTGGGACCA GTCTTCAGTA TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006530203.1
CDS	157..6369
Translation	MLKFKYGVRNPPEASASEPIASRASRLNLFFQGKPPLMTQQQMSALSREGMLDALFALFEECSQPALMKMKHVSSFVQKYSDTIAELRELQPSARDFEVRSLVGCGHFAEVQVVREKATGDVYAMKIMKKKALLAQEQVSFFEEERNILSRSTSPWIPQLQYAFQDKNNLYLVMEYQPGGDLLSLLNRYEDQLDESMIQFYLAELILAVHSVHQMGYVHRDIKPENILIDRTGHIKLVDFGSAAKMNSNKVDAKLPIGTPDYMAPEVLTVMNEDRRGTYGLDCDWWSVGVVAYEMVYGKTPFTEGTSARTFNNIMNFQRFLKFPDDPKVSSELLDLLQSLLCVQKERLKFEGLCCHPFFARTDWNNIRNSPPPFVPTLKSDDDTSNFDEPEKNSWVSSSVCQLSPSGFSGEELPFVGFSYSKALGYLGRSESVVSSLDSPAKVSSMEKKLLIKSKELQDSQDKCHKMEQEMTRLHRRVSEVEAVLSQKEVELKASETQRSLLEQDLATYITECSSLKRSLEQARMEVSQEDDKALQLLHDIREQSRKLQEIKEQEYQAQVEEMRLMMNQLEEDLVSARRRSDLYESELRESRLAAEEFKRKANECQHKLMKAKDQGKPEVGEYSKLEKINAEQQLKIQELQEKLEKAVKASTEATELLQNIRQAKERAERELEKLHNREDSSEGIKKKLVEAEERRHSLENKVKRLETMERRENRLKDDIQTKSEQIQQMADKILELEEKHREAQVSAQHLEVHLKQKEQHYEEKIKVLDNQIKKDLADKESLENMMQRHEEEAHEKGKILSEQKAMINAMDSKIRSLEQRIVELSEANKLAANSSLFTQRNMKAQEEMISELRQQKFYLETQAGKLEAQNRKLEEQLEKISHQDHSDKSRLLELETRLREVSLEHEEQKLELKRQLTELQLSLQERESQLTALQAARAALESQLRQAKTELEETTAEAEEEIQALTAHRDEIQRKFDALRNSCTVITDLEEQLNQLTEDNAELNNQNFYLSKQLDEASGANDEIVQLRSEVDHLRREITEREMQLTSQKQTMEALKTTCTMLEEQVLDLEALNDELLEKERQWEAWRSVLGDEKSQFECRVRELQRMLDTEKQSRARADQRITESRQVVELAVKEHKAEILALQQALKEQKLKAESLSDKLNDLEKKHAMLEMNARSLQQKLETERELKQRLLEEQAKLQQQMDLQKNHIFRLTQGLQEALDRADLLKTERSDLEYQLENIQVLYSHEKVKMEGTISQQTKLIDFLQAKMDQPAKKKKGLFSRRKEDPALPTQVPLQYNELKLALEKEKARCAELEEALQKTRIELRSAREEAAHRKATDHPHPSTPATARQQIAMSAIVRSPEHQPSAMSLLAPPSSRRKESSTPEEFSRRLKERMHHNIPHRFNVGLNMRATKCAVCLDTVHFGRQASKCLECQVMCHPKCSTCLPATCGLPAEYATHFTEAFCRDKMNSPGLQSKEPGSSLHLEGWMKVPRNNKRGQQGWDRKYIVLEGSKVLIYDNEAREAGQRPVEEFELCLPDGDVSIHGAVGASELANTAKADVPYILKMESHPHTTCWPGRTLYLLAPSFPDKQRWVTALESVVAGGRVSREKAEADAKLLGNSLLKLEGDDRLDMNCTLPFSDQVVLVGTEEGLYALNVLKNSLTHIPGIGAVFQIYIIKDLEKLLMIAGEERALCLVDVKKVKQSLAQSHLPAQPDVSPNIFEAVKGCHLFAAGKIENSLCICAAMPSKVVILRYNDNLSKYCIRKEIETSEPCSCIHFTNYSILIGTNKFYEIDMKQYTLDEFLDKNDHSLAPAVFASSSNSFPVSIVQANSAGQREEYLLCFHEFGVFVDSYGRRSRTDDLKWSRLPLAFAYREPYLFVTHFNSLEVIEIQARSSLGSPARAYLEIPNPRYLGPAISSGAIYLASSYQDKLRVICCKGNLVKESGTEQHRVPSTSRSSPNKRGPPTYNEHITKRVASSPAPPEGPSHPREPSTPHRYRDREGRTELRRDKSPGRPLEREKSPGRMLSTRRERSPGRLFEDSSRGRLPAGAVRTPLSQVNKVWDQSSV

Target ORF information:

RefSeq Version	XM_006530140.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus citron (Cit), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006530140.3

ORF Insert Sequence:

ATGTTGAAGT TCAAGTATGG TGTGCGGAAC CCGCCGGAGG CCAGTGCCTC CGAGCCCATT 
GCCAGTCGGG CCTCCAGGCT AAATCTCTTC TTCCAGGGGA AACCGCCCCT CATGACTCAA 
CAGCAGATGT CTGCTCTTTC CCGGGAAGGG ATGCTAGACG CCCTCTTCGC TCTCTTTGAA 
GAGTGCAGCC AACCCGCCCT GATGAAGATG AAGCACGTGA GCAGCTTTGT CCAGAAGTAT 
TCCGACACCA TAGCCGAGTT GCGGGAGCTG CAGCCGTCGG CGAGAGACTT CGAAGTTCGA 
AGCCTTGTGG GCTGTGGTCA CTTCGCTGAA GTGCAGGTGG TTAGAGAGAA GGCGACCGGG 
GACGTCTATG CCATGAAAAT CATGAAGAAG AAGGCTTTGC TGGCCCAGGA ACAGGTTTCA 
TTTTTCGAGG AGGAGAGGAA CATATTATCT CGGAGCACGA GTCCTTGGAT CCCCCAGTTA 
CAGTACGCCT TTCAGGACAA AAATAACCTT TACCTGGTCA TGGAATATCA GCCTGGAGGG 
GATTTGCTGT CGCTTCTGAA CAGATACGAG GACCAATTAG ATGAGAGCAT GATCCAGTTT 
TACCTTGCTG AGCTGATTTT GGCTGTCCAC AGCGTGCACC AGATGGGATA TGTGCATCGA 
GACATCAAGC CCGAGAACAT CCTCATCGAC CGGACGGGAC ACATCAAGCT GGTGGATTTT 
GGATCAGCCG CTAAGATGAA TTCAAATAAG GTGGATGCCA AACTCCCCAT TGGGACCCCG 
GATTACATGG CTCCGGAAGT GTTGACCGTG ATGAACGAGG ACCGAAGGGG CACATACGGC 
TTGGACTGTG ACTGGTGGTC TGTCGGAGTT GTTGCTTATG AGATGGTTTA TGGGAAGACC 
CCATTCACAG AGGGAACCTC CGCCCGGACC TTCAACAACA TCATGAACTT CCAGCGGTTT 
TTGAAGTTCC CAGATGACCC CAAAGTTAGC AGTGAGCTCC TTGATCTGCT TCAGAGTCTG 
CTGTGTGTCC AGAAAGAGAG ACTGAAGTTC GAGGGTCTCT GCTGCCACCC TTTCTTTGCC 
AGAACGGACT GGAACAACAT CCGTAACTCT CCTCCCCCCT TCGTCCCCAC CCTCAAGTCT 
GACGATGACA CCTCCAATTT TGATGAACCA GAGAAGAATT CGTGGGTTTC ATCCTCTGTG 
TGCCAGCTGA GCCCCTCGGG CTTCTCAGGC GAAGAGCTGC CGTTTGTGGG ATTTTCGTAC 
AGCAAGGCAC TGGGGTATCT TGGTAGATCT GAGTCTGTCG TGTCGAGTCT GGACTCCCCT 
GCCAAGGTTA GCTCCATGGA AAAGAAACTT CTCATCAAAA GCAAAGAGCT CCAAGACTCC 
CAGGACAAGT GTCACAAGAT GGAGCAGGAA ATGACCCGGT TACATCGCAG AGTGTCAGAG 
GTGGAGGCTG TGCTTAGTCA GAAGGAGGTG GAGCTGAAGG CCTCTGAGAC TCAGAGATCC 
CTCCTGGAGC AGGACCTTGC TACCTACATC ACAGAATGCA GTAGCTTAAA GCGAAGTTTG 
GAGCAAGCGC GGATGGAGGT GTCCCAGGAG GATGACAAAG CTCTGCAGCT TCTCCACGAC 
ATCCGAGAGC AGAGCCGGAA GCTCCAGGAG ATCAAGGAGC AGGAGTACCA GGCTCAGGTG 
GAGGAGATGA GGCTGATGAT GAATCAGCTG GAAGAAGACC TGGTGTCAGC CCGCAGACGC 
AGCGATCTCT ACGAGTCTGA GCTGAGGGAG TCTCGGCTTG CCGCCGAGGA ATTCAAGCGG 
AAGGCAAACG AATGTCAGCA CAAACTGATG AAGGCTAAGG ACCAAGGGAA GCCTGAAGTG 
GGAGAATATT CCAAACTGGA GAAGATCAAT GCTGAGCAGC AGCTGAAGAT CCAGGAGCTC 
CAGGAGAAGC TGGAGAAGGC TGTAAAAGCC AGCACAGAGG CCACCGAGCT CCTGCAGAAT 
ATCCGCCAGG CAAAGGAGCG AGCAGAGCGG GAGCTAGAGA AGCTACACAA CCGGGAAGAC 
TCCTCCGAGG GCATCAAAAA GAAGCTGGTG GAAGCCGAGG AACGCCGCCA CTCCCTGGAG 
AACAAGGTAA AGAGACTAGA GACCATGGAG CGTAGAGAGA ACAGACTGAA GGATGACATC 
CAGACAAAGT CCGAACAGAT CCAGCAGATG GCTGATAAAA TTCTGGAGCT GGAGGAGAAA 
CATCGGGAGG CTCAGGTCTC AGCTCAACAT CTAGAAGTAC ACTTGAAACA GAAAGAACAG 
CACTACGAGG AAAAGATCAA GGTATTGGAC AATCAGATAA AGAAGGACCT GGCCGACAAG 
GAGAGCCTGG AGAACATGAT GCAGAGACAC GAGGAGGAGG CCCACGAGAA GGGCAAGATC 
CTCAGCGAGC AGAAGGCGAT GATCAACGCG ATGGATTCCA AGATCCGATC CCTGGAGCAG 
AGGATCGTGG AGCTGTCGGA AGCCAACAAG CTTGCGGCAA ACAGCAGTCT CTTCACCCAG 
AGGAACATGA AGGCCCAGGA AGAGATGATC TCAGAACTCA GGCAGCAGAA ATTTTACCTG 
GAGACGCAGG CCGGAAAGCT GGAGGCCCAG AACCGAAAGC TGGAAGAGCA ACTGGAGAAG 
ATCAGCCACC AAGATCACAG TGACAAGAGT CGGCTGCTGG AGCTGGAAAC AAGGCTGAGG 
GAGGTCAGCC TGGAGCACGA GGAGCAGAAG CTGGAGCTGA AGCGGCAGCT CACGGAGCTG 
CAGCTGTCCC TGCAGGAGCG CGAGTCCCAG CTGACGGCCC TGCAGGCCGC CCGGGCAGCG 
CTGGAGAGCC AGCTCCGCCA GGCGAAGACA GAGCTGGAGG AGACAACCGC GGAAGCGGAG 
GAGGAGATCC AGGCGCTCAC GGCACATCGA GATGAGATCC AGCGCAAATT CGATGCCCTT 
CGCAACAGCT GCACGGTCAT CACCGACCTG GAGGAGCAGC TGAACCAGCT CACCGAGGAC 
AACGCCGAGC TCAACAACCA AAACTTCTAC CTGTCCAAAC AACTCGATGA GGCTTCCGGG 
GCCAATGACG AGATTGTGCA GCTGCGGAGT GAGGTGGACC ATCTCCGCCG TGAGATCACG 
GAGCGGGAGA TGCAGCTCAC CAGCCAGAAG CAAACGATGG AGGCTCTGAA GACGACATGC 
ACCATGCTGG AGGAGCAGGT CCTGGACCTG GAGGCCCTGA ACGACGAGCT GCTGGAGAAG 
GAGCGCCAGT GGGAGGCCTG GCGGAGCGTC CTCGGCGACG AGAAGTCCCA GTTCGAGTGT 
CGAGTTCGAG AGCTACAGAG GATGCTGGAC ACCGAGAAGC AGAGCAGGGC TAGGGCCGAT 
CAGCGGATCA CCGAGTCGCG CCAGGTGGTG GAGTTGGCGG TGAAGGAACA CAAGGCCGAG 
ATTCTTGCTC TGCAGCAGGC TCTCAAGGAG CAGAAGCTCA AAGCCGAGAG CCTGTCGGAC 
AAGCTCAACG ACCTGGAGAA GAAGCACGCC ATGCTGGAGA TGAACGCCCG GAGCTTACAG 
CAGAAACTAG AGACAGAGCG GGAGCTCAAA CAGAGGCTTC TGGAGGAGCA AGCCAAATTG 
CAGCAGCAGA TGGACCTGCA GAAGAACCAC ATCTTCAGAC TGACGCAAGG GCTGCAGGAG 
GCGCTGGACC GGGCGGATCT GCTGAAGACA GAAAGGAGCG ACCTGGAGTA CCAGCTGGAA 
AACATTCAGG TTCTCTACTC TCACGAGAAA GTGAAAATGG AAGGCACAAT CTCTCAGCAA 
ACCAAACTCA TTGATTTCCT GCAAGCCAAA ATGGACCAGC CTGCTAAAAA GAAAAAGGGT 
TTATTTAGTC GACGGAAAGA GGACCCTGCT TTGCCCACAC AGGTTCCTCT GCAGTACAAT 
GAGCTGAAGC TAGCCCTGGA AAAGGAGAAA GCCCGATGCG CGGAGCTGGA GGAGGCCCTT 
CAGAAGACCC GCATCGAACT CCGCTCTGCG CGGGAGGAAG CTGCCCACCG CAAAGCCACG 
GACCACCCGC ACCCGTCTAC GCCAGCCACT GCGAGGCAGC AGATCGCCAT GTCTGCCATT 
GTGCGGTCGC CCGAGCACCA GCCCAGTGCC ATGAGCCTGC TTGCCCCACC ATCCAGCCGC 
AGAAAGGAGT CGTCAACTCC AGAGGAATTC AGCCGGCGTC TGAAAGAGCG CATGCACCAC 
AACATCCCTC ACCGGTTTAA TGTGGGCCTG AACATGAGAG CCACCAAGTG CGCCGTGTGT 
CTGGATACTG TGCACTTTGG ACGCCAGGCA TCCAAATGCC TCGAATGTCA AGTGATGTGT 
CACCCCAAAT GCTCCACCTG CTTGCCCGCT ACCTGTGGCC TGCCAGCTGA ATATGCCACA 
CACTTCACTG AGGCCTTCTG CCGGGACAAA ATGAACTCCC CGGGGCTCCA GAGCAAGGAG 
CCTGGCAGCA GCTTGCACCT GGAAGGGTGG ATGAAAGTGC CCAGAAATAA CAAACGGGGA 
CAGCAAGGCT GGGACAGGAA GTACATTGTT CTGGAGGGGT CAAAAGTCCT CATCTATGAC 
AATGAAGCCA GAGAAGCTGG ACAGAGGCCG GTGGAAGAAT TTGAGCTGTG CCTTCCTGAC 
GGGGACGTAT CTATTCATGG TGCCGTTGGT GCTTCAGAAC TTGCAAATAC CGCCAAAGCA 
GATGTCCCAT ACATCCTGAA GATGGAGTCT CACCCACACA CCACCTGCTG GCCTGGGAGA 
ACCCTCTACT TGCTAGCACC CAGCTTCCCC GACAAGCAGC GCTGGGTCAC CGCCTTAGAA 
TCTGTCGTCG CAGGTGGGAG AGTTTCTAGG GAAAAGGCCG AAGCCGATGC TAAATTACTT 
GGAAACTCTC TGCTGAAACT GGAGGGCGAT GACCGGCTTG ACATGAACTG CACCCTGCCC 
TTCAGTGACC AGGTAGTGCT GGTGGGCACG GAGGAAGGCC TCTACGCCCT GAATGTCTTG 
AAAAACTCCT TAACCCACAT CCCAGGGATT GGCGCAGTCT TCCAAATTTA CATCATCAAG 
GACCTGGAGA AGCTGCTCAT GATAGCAGGG GAAGAGCGGG CTCTGTGCCT GGTGGACGTG 
AAGAAGGTGA AGCAGTCCCT GGCGCAGTCA CACCTGCCTG CCCAGCCCGA CGTCTCCCCC 
AACATATTCG AAGCCGTCAA AGGCTGCCAC TTGTTCGCTG CTGGCAAGAT CGAGAACAGC 
CTGTGCATCT GCGCCGCTAT GCCAAGCAAA GTCGTCATCC TCCGCTACAA TGACAACCTC 
AGCAAGTACT GCATCCGCAA GGAGATCGAG ACCTCAGAGC CCTGCAGCTG TATCCACTTC 
ACCAACTACA GCATCCTCAT TGGGACCAAC AAATTCTATG AGATCGACAT GAAGCAGTAC 
ACGCTTGACG AGTTCCTGGA CAAGAACGAC CATTCCTTGG CACCTGCTGT GTTCGCCTCC 
TCGTCCAACA GCTTCCCTGT CTCCATTGTG CAGGCGAACA GCGCCGGGCA GCGAGAAGAA 
TACCTGCTGT GCTTCCACGA ATTTGGGGTG TTCGTGGATT CTTACGGAAG ACGTAGCCGC 
ACAGATGATC TCAAGTGGAG TCGCTTACCT CTGGCCTTCG CCTACAGAGA ACCTTATCTG 
TTTGTGACTC ACTTCAACTC CCTGGAAGTC ATTGAGATCC AGGCCAGATC CTCACTGGGG 
AGCCCTGCCC GAGCATATCT GGAAATTCCA AACCCTCGCT ACCTGGGCCC CGCGATTTCC 
TCCGGAGCGA TTTACCTGGC CTCCTCATAC CAGGACAAGT TAAGGGTCAT ATGCTGCAAA 
GGAAACCTCG TGAAGGAGTC AGGCACTGAG CAGCACCGGG TGCCCTCCAC CTCCCGCAGC 
AGCCCCAACA AGCGAGGCCC ACCAACATAC AACGAGCACA TCACCAAACG CGTGGCCTCC 
AGCCCGGCGC CACCGGAAGG CCCCAGCCAC CCCCGAGAGC CAAGCACACC GCACCGCTAC 
CGAGACAGAG AGGGTCGGAC AGAGCTGCGC AGGGACAAGT CTCCAGGCCG CCCTCTGGAG 
CGGGAGAAGT CCCCAGGCCG AATGCTCAGC ACTAGGAGAG AGCGGTCCCC AGGGAGACTG 
TTTGAAGACA GCAGCAGGGG CCGGCTGCCT GCAGGAGCAG TGAGGACCCC ACTGTCCCAG 
GTTAACAAGG TCTGGGACCA GTCTTCAGTA TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cit ( XM_006530142.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006530205.1 Mus musculus citron (Cit), transcript variant X16, mRNA.

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CloneID	OMu35782
Clone ID Related Accession (Same CDS sequence)	XM_006530142.3
Accession Version	XM_006530142.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6120bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	citron Rho-interacting kinase isoform X12
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000071.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006530142.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTGAAGT TCAAGTATGG TGTGCGGAAC CCGCCGGAGG CCAGTGCCTC CGAGCCCATT 
GCCAGTCGGG CCTCCAGGCT AAATCTCTTC TTCCAGGGGA AACCGCCCCT CATGACTCAA 
CAGCAGATGT CTGCTCTTTC CCGGGAAGGG ATGCTAGACG CCCTCTTCGC TCTCTTTGAA 
GAGTGCAGCC AACCCGCCCT GATGAAGATG AAGCACGTGA GCAGCTTTGT CCAGAAGTAT 
TCCGACACCA TAGCCGAGTT GCGGGAGCTG CAGCCGTCGG CGAGAGACTT CGAAGTTCGA 
AGCCTTGTGG GCTGTGGTCA CTTCGCTGAA GTGCAGGTGG TTAGAGAGAA GGCGACCGGG 
GACGTCTATG CCATGAAAAT CATGAAGAAG AAGGCTTTGC TGGCCCAGGA ACAGGTTTCA 
TTTTTCGAGG AGGAGAGGAA CATATTATCT CGGAGCACGA GTCCTTGGAT CCCCCAGTTA 
CAGTACGCCT TTCAGGACAA AAATAACCTT TACCTGGTCA TGGAATATCA GCCTGGAGGG 
GATTTGCTGT CGCTTCTGAA CAGATACGAG GACCAATTAG ATGAGAGCAT GATCCAGTTT 
TACCTTGCTG AGCTGATTTT GGCTGTCCAC AGCGTGCACC AGATGGGATA TGTGCATCGA 
GACATCAAGC CCGAGAACAT CCTCATCGAC CGGACGGGAC ACATCAAGCT GGTGGATTTT 
GGATCAGCCG CTAAGATGAA TTCAAATAAG GTGGATGCCA AACTCCCCAT TGGGACCCCG 
GATTACATGG CTCCGGAAGT GTTGACCGTG ATGAACGAGG ACCGAAGGGG CACATACGGC 
TTGGACTGTG ACTGGTGGTC TGTCGGAGTT GTTGCTTATG AGATGGTTTA TGGGAAGACC 
CCATTCACAG AGGGAACCTC CGCCCGGACC TTCAACAACA TCATGAACTT CCAGCGGTTT 
TTGAAGTTCC CAGATGACCC CAAAGTTAGC AGTGAGCTCC TTGATCTGCT TCAGAGTCTG 
CTGTGTGTCC AGAAAGAGAG ACTGAAGTTC GAGGGTCTCT GCTGCCACCC TTTCTTTGCC 
AGAACGGACT GGAACAACAT CCGTAACTCT CCTCCCCCCT TCGTCCCCAC CCTCAAGTCT 
GACGATGACA CCTCCAATTT TGATGAACCA GAGAAGAATT CGTGGGTTTC ATCCTCTGTG 
TGCCAGCTGA GCCCCTCGGG CTTCTCAGGC GAAGAGCTGC CGTTTGTGGG ATTTTCGTAC 
AGCAAGGCAC TGGGGTATCT TGGTAGATCT GAGTCTGTCG TGTCGAGTCT GGACTCCCCT 
GCCAAGGTTA GCTCCATGGA AAAGAAACTT CTCATCAAAA GCAAAGAGCT CCAAGACTCC 
CAGGACAAGT GTCACAAGAT GGAGCAGGAA ATGACCCGGT TACATCGCAG AGTGTCAGAG 
GTGGAGGCTG TGCTTAGTCA GAAGGAGGTG GAGCTGAAGG CCTCTGAGAC TCAGAGATCC 
CTCCTGGAGC AGGACCTTGC TACCTACATC ACAGAATGCA GTAGCTTAAA GCGAAGTTTG 
GAGCAAGCGC GGATGGAGGT GTCCCAGGAG GATGACAAAG CTCTGCAGCT TCTCCACGAC 
ATCCGAGAGC AGAGCCGGAA GCTCCAGGAG ATCAAGGAGC AGGAGTACCA GGCTCAGGTG 
GAGGAGATGA GGCTGATGAT GAATCAGCTG GAAGAAGACC TGGTGTCAGC CCGCAGACGC 
AGCGATCTCT ACGAGTCTGA GCTGAGGGAG TCTCGGCTTG CCGCCGAGGA ATTCAAGCGG 
AAGGCAAACG AATGTCAGCA CAAACTGATG AAGGTAGTCA GCCACCCTCC AAGAGGGGAC 
TCCGGGGGCA CGGCCCTGGA TGATCTTCAC AAGATGCAAG GCCACGCTGG CTTAACGAGT 
GCTAAGGACC AAGGGAAGCC TGAAGTGGGA GAATATTCCA AACTGGAGAA GATCAATGCT 
GAGCAGCAGC TGAAGATCCA GGAGCTCCAG GAGAAGCTGG AGAAGGCTGT AAAAGCCAGC 
ACAGAGGCCA CCGAGCTCCT GCAGAATATC CGCCAGGCAA AGGAGCGAGC AGAGCGGGAG 
CTAGAGAAGC TACACAACCG GGAAGACTCC TCCGAGGGCA TCAAAAAGAA GCTGGTGGAA 
GCCGAGGAAC GCCGCCACTC CCTGGAGAAC AAGGTAAAGA GACTAGAGAC CATGGAGCGT 
AGAGAGAACA GACTGAAGGA TGACATCCAG ACAAAGTCCG AACAGATCCA GCAGATGGCT 
GATAAAATTC TGGAGCTGGA GGAGAAACAT CGGGAGGCTC AGGTCTCAGC TCAACATCTA 
GAAGTACACT TGAAACAGAA AGAACAGCAC TACGAGGAAA AGATCAAGGT ATTGGACAAT 
CAGATAAAGA AGGACCTGGC CGACAAGGAG AGCCTGGAGA ACATGATGCA GAGACACGAG 
GAGGAGGCCC ACGAGAAGGG CAAGATCCTC AGCGAGCAGA AGGCGATGAT CAACGCGATG 
GATTCCAAGA TCCGATCCCT GGAGCAGAGG ATCGTGGAGC TGTCGGAAGC CAACAAGCTT 
GCGGCAAACA GCAGTCTCTT CACCCAGAGG AACATGAAGG CCCAGGAAGA GATGATCTCA 
GAACTCAGGC AGCAGAAATT TTACCTGGAG ACGCAGGCCG GAAAGCTGGA GGCCCAGAAC 
CGAAAGCTGG AAGAGCAACT GGAGAAGATC AGCCACCAAG ATCACAGTGA CAAGAGTCGG 
CTGCTGGAGC TGGAAACAAG GCTGAGGGAG GTCAGCCTGG AGCACGAGGA GCAGAAGCTG 
GAGCTGAAGC GGCAGCTCAC GGAGCTGCAG CTGTCCCTGC AGGAGCGCGA GTCCCAGCTG 
ACGGCCCTGC AGGCCGCCCG GGCAGCGCTG GAGAGCCAGC TCCGCCAGGC GAAGACAGAG 
CTGGAGGAGA CAACCGCGGA AGCGGAGGAG GAGATCCAGG CGCTCACGGC ACATCGAGAT 
GAGATCCAGC GCAAATTCGA TGCCCTTCGC AACAGCTGCA CGGTCATCAC CGACCTGGAG 
GAGCAGCTGA ACCAGCTCAC CGAGGACAAC GCCGAGCTCA ACAACCAAAA CTTCTACCTG 
TCCAAACAAC TCGATGAGGC TTCCGGGGCC AATGACGAGA TTGTGCAGCT GCGGAGTGAG 
GTGGACCATC TCCGCCGTGA GATCACGGAG CGGGAGATGC AGCTCACCAG CCAGAAGCAA 
GCACAACTCA GTGCTCCTGA TTTGCAGACG ATGGAGGCTC TGAAGACGAC ATGCACCATG 
CTGGAGGAGC AGGTCCTGGA CCTGGAGGCC CTGAACGACG AGCTGCTGGA GAAGGAGCGC 
CAGTGGGAGG CCTGGCGGAG CGTCCTCGGC GACGAGAAGT CCCAGTTCGA GTGTCGAGTT 
CGAGAGCTAC AGAGGATGCT GGACACCGAG AAGCAGAGCA GGGCTAGGGC CGATCAGCGG 
ATCACCGAGT CGCGCCAGGT GGTGGAGTTG GCGGTGAAGG AACACAAGGC CGAGATTCTT 
GCTCTGCAGC AGGCTCTCAA GGAGCAGAAG CTCAAAGCCG AGAGCCTGTC GGACAAGCTC 
AACGACCTGG AGAAGAAGCA CGCCATGCTG GAGATGAACG CCCGGAGCTT ACAGCAGAAA 
CTAGAGACAG AGCGGGAGCT CAAACAGAGG CTTCTGGAGG AGCAAGCCAA ATTGCAGCAG 
CAGATGGACC TGCAGAAGAA CCACATCTTC AGACTGACGC AAGGGCTGCA GGAGGCGCTG 
GACCGGGCGG ATCTGCTGAA GACAGAAAGG AGCGACCTGG AGTACCAGCT GGAAAACATT 
CAGGTTCTCT ACTCTCACGA GAAAGTGAAA ATGGAAGGCA CAATCTCTCA GCAAACCAAA 
CTCATTGATT TCCTGCAAGC CAAAATGGAC CAGCCTGCTA AAAAGAAAAA GGGTTTATTT 
AGTCGACGGA AAGAGGACCC TGCTTTGCCC ACACAGGTTC CTCTGCAGTA CAATGAGCTG 
AAGCTAGCCC TGGAAAAGGA GAAAGCCCGA TGCGCGGAGC TGGAGGAGGC CCTTCAGAAG 
ACCCGCATCG AACTCCGCTC TGCGCGGGAG GAAGCTGCCC ACCGCAAAGC CACGGACCAC 
CCGCACCCGT CTACGCCAGC CACTGCGAGG CAGCAGATCG CCATGTCTGC CATTGTGCGG 
TCGCCCGAGC ACCAGCCCAG TGCCATGAGC CTGCTTGCCC CACCATCCAG CCGCAGAAAG 
GAGTCGTCAA CTCCAGAGGA ATTCAGCCGG CGTCTGAAAG AGCGCATGCA CCACAACATC 
CCTCACCGGT TTAATGTGGG CCTGAACATG AGAGCCACCA AGTGCGCCGT GTGTCTGGAT 
ACTGTGCACT TTGGACGCCA GGCATCCAAA TGCCTCGAAT GTCAAGTGAT GTGTCACCCC 
AAATGCTCCA CCTGCTTGCC CGCTACCTGT GGCCTGCCAG CTGAATATGC CACACACTTC 
ACTGAGGCCT TCTGCCGGGA CAAAATGAAC TCCCCGGGGC TCCAGAGCAA GGAGCCTGGC 
AGCAGCTTGC ACCTGGAAGG GTGGATGAAA GTGCCCAGAA ATAACAAACG GGGACAGCAA 
GGCTGGGACA GGAAGTACAT TGTTCTGGAG GGGTCAAAAG TCCTCATCTA TGACAATGAA 
GCCAGAGAAG CTGGACAGAG GCCGGTGGAA GAATTTGAGC TGTGCCTTCC TGACGGGGAC 
GTATCTATTC ATGGTGCCGT TGGTGCTTCA GAACTTGCAA ATACCGCCAA AGCAGATGTC 
CCATACATCC TGAAGATGGA GTCTCACCCA CACACCACCT GCTGGCCTGG GAGAACCCTC 
TACTTGCTAG CACCCAGCTT CCCCGACAAG CAGCGCTGGG TCACCGCCTT AGAATCTGTC 
GTCGCAGGTG GGAGAGTTTC TAGGGAAAAG GCCGAAGCCG ATGCTGCACG TGACCATACT 
TCGAGTGAGC ATCAGCCAGT GTGGGTTGAG AAATTACTTG GAAACTCTCT GCTGAAACTG 
GAGGGCGATG ACCGGCTTGA CATGAACTGC ACCCTGCCCT TCAGTGACCA GGTAGTGCTG 
GTGGGCACGG AGGAAGGCCT CTACGCCCTG AATGTCTTGA AAAACTCCTT AACCCACATC 
CCAGGGATTG GCGCAGTCTT CCAAATTTAC ATCATCAAGG ACCTGGAGAA GCTGCTCATG 
ATAGCAGGGG AAGAGCGGGC TCTGTGCCTG GTGGACGTGA AGAAGGTGAA GCAGTCCCTG 
GCGCAGTCAC ACCTGCCTGC CCAGCCCGAC GTCTCCCCCA ACATATTCGA AGCCGTCAAA 
GGCTGCCACT TGTTCGCTGC TGGCAAGATC GAGAACAGCC TGTGCATCTG CGCCGCTATG 
CCAAGCAAAG TCGTCATCCT CCGCTACAAT GACAACCTCA GCAAGTACTG CATCCGCAAG 
GAGATCGAGA CCTCAGAGCC CTGCAGCTGT ATCCACTTCA CCAACTACAG CATCCTCATT 
GGGACCAACA AATTCTATGA GATCGACATG AAGCAGTACA CGCTTGACGA GTTCCTGGAC 
AAGAACGACC ATTCCTTGGC ACCTGCTGTG TTCGCCTCCT CGTCCAACAG CTTCCCTGTC 
TCCATTGTGC AGGCGAACAG CGCCGGGCAG CGAGAAGAAT ACCTGCTGTG CTTCCACGAA 
TTTGGGGTGT TCGTGGATTC TTACGGAAGA CGTAGCCGCA CAGATGATCT CAAGTGGAGT 
CGCTTACCTC TGGCCTTCGC CTACAGAGAA CCTTATCTGT TTGTGACTCA CTTCAACTCC 
CTGGAAGTCA TTGAGATCCA GGCCAGATCC TCACTGGGGA GCCCTGCCCG AGCATATCTG 
GAAATTCCAA ACCCTCGCTA CCTGGGCCCC GCGATTTCCT CCGGAGCGAT TTACCTGGCC 
TCCTCATACC AGGACAAGTT AAGGGTCATA TGCTGCAAAG GAAACCTCGT GAAGGAGTCA 
GGCACTGAGC AGCACCGGGT GCCCTCCACC TCCCGCAGAT GTTTCAGGCT GCTGATTCTT 
CGTGGTGGCT GTCAGTTCTC CCATCGATCC AGGGAGAGGC ACGGGCCCAG GGCATGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006530205.1
CDS	158..6277
Translation	MLKFKYGVRNPPEASASEPIASRASRLNLFFQGKPPLMTQQQMSALSREGMLDALFALFEECSQPALMKMKHVSSFVQKYSDTIAELRELQPSARDFEVRSLVGCGHFAEVQVVREKATGDVYAMKIMKKKALLAQEQVSFFEEERNILSRSTSPWIPQLQYAFQDKNNLYLVMEYQPGGDLLSLLNRYEDQLDESMIQFYLAELILAVHSVHQMGYVHRDIKPENILIDRTGHIKLVDFGSAAKMNSNKVDAKLPIGTPDYMAPEVLTVMNEDRRGTYGLDCDWWSVGVVAYEMVYGKTPFTEGTSARTFNNIMNFQRFLKFPDDPKVSSELLDLLQSLLCVQKERLKFEGLCCHPFFARTDWNNIRNSPPPFVPTLKSDDDTSNFDEPEKNSWVSSSVCQLSPSGFSGEELPFVGFSYSKALGYLGRSESVVSSLDSPAKVSSMEKKLLIKSKELQDSQDKCHKMEQEMTRLHRRVSEVEAVLSQKEVELKASETQRSLLEQDLATYITECSSLKRSLEQARMEVSQEDDKALQLLHDIREQSRKLQEIKEQEYQAQVEEMRLMMNQLEEDLVSARRRSDLYESELRESRLAAEEFKRKANECQHKLMKVVSHPPRGDSGGTALDDLHKMQGHAGLTSAKDQGKPEVGEYSKLEKINAEQQLKIQELQEKLEKAVKASTEATELLQNIRQAKERAERELEKLHNREDSSEGIKKKLVEAEERRHSLENKVKRLETMERRENRLKDDIQTKSEQIQQMADKILELEEKHREAQVSAQHLEVHLKQKEQHYEEKIKVLDNQIKKDLADKESLENMMQRHEEEAHEKGKILSEQKAMINAMDSKIRSLEQRIVELSEANKLAANSSLFTQRNMKAQEEMISELRQQKFYLETQAGKLEAQNRKLEEQLEKISHQDHSDKSRLLELETRLREVSLEHEEQKLELKRQLTELQLSLQERESQLTALQAARAALESQLRQAKTELEETTAEAEEEIQALTAHRDEIQRKFDALRNSCTVITDLEEQLNQLTEDNAELNNQNFYLSKQLDEASGANDEIVQLRSEVDHLRREITEREMQLTSQKQAQLSAPDLQTMEALKTTCTMLEEQVLDLEALNDELLEKERQWEAWRSVLGDEKSQFECRVRELQRMLDTEKQSRARADQRITESRQVVELAVKEHKAEILALQQALKEQKLKAESLSDKLNDLEKKHAMLEMNARSLQQKLETERELKQRLLEEQAKLQQQMDLQKNHIFRLTQGLQEALDRADLLKTERSDLEYQLENIQVLYSHEKVKMEGTISQQTKLIDFLQAKMDQPAKKKKGLFSRRKEDPALPTQVPLQYNELKLALEKEKARCAELEEALQKTRIELRSAREEAAHRKATDHPHPSTPATARQQIAMSAIVRSPEHQPSAMSLLAPPSSRRKESSTPEEFSRRLKERMHHNIPHRFNVGLNMRATKCAVCLDTVHFGRQASKCLECQVMCHPKCSTCLPATCGLPAEYATHFTEAFCRDKMNSPGLQSKEPGSSLHLEGWMKVPRNNKRGQQGWDRKYIVLEGSKVLIYDNEAREAGQRPVEEFELCLPDGDVSIHGAVGASELANTAKADVPYILKMESHPHTTCWPGRTLYLLAPSFPDKQRWVTALESVVAGGRVSREKAEADAARDHTSSEHQPVWVEKLLGNSLLKLEGDDRLDMNCTLPFSDQVVLVGTEEGLYALNVLKNSLTHIPGIGAVFQIYIIKDLEKLLMIAGEERALCLVDVKKVKQSLAQSHLPAQPDVSPNIFEAVKGCHLFAAGKIENSLCICAAMPSKVVILRYNDNLSKYCIRKEIETSEPCSCIHFTNYSILIGTNKFYEIDMKQYTLDEFLDKNDHSLAPAVFASSSNSFPVSIVQANSAGQREEYLLCFHEFGVFVDSYGRRSRTDDLKWSRLPLAFAYREPYLFVTHFNSLEVIEIQARSSLGSPARAYLEIPNPRYLGPAISSGAIYLASSYQDKLRVICCKGNLVKESGTEQHRVPSTSRRCFRLLILRGGCQFSHRSRERHGPRAC

Target ORF information:

RefSeq Version	XM_006530142.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus citron (Cit), transcript variant X16, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006530142.3

ORF Insert Sequence:

ATGTTGAAGT TCAAGTATGG TGTGCGGAAC CCGCCGGAGG CCAGTGCCTC CGAGCCCATT 
GCCAGTCGGG CCTCCAGGCT AAATCTCTTC TTCCAGGGGA AACCGCCCCT CATGACTCAA 
CAGCAGATGT CTGCTCTTTC CCGGGAAGGG ATGCTAGACG CCCTCTTCGC TCTCTTTGAA 
GAGTGCAGCC AACCCGCCCT GATGAAGATG AAGCACGTGA GCAGCTTTGT CCAGAAGTAT 
TCCGACACCA TAGCCGAGTT GCGGGAGCTG CAGCCGTCGG CGAGAGACTT CGAAGTTCGA 
AGCCTTGTGG GCTGTGGTCA CTTCGCTGAA GTGCAGGTGG TTAGAGAGAA GGCGACCGGG 
GACGTCTATG CCATGAAAAT CATGAAGAAG AAGGCTTTGC TGGCCCAGGA ACAGGTTTCA 
TTTTTCGAGG AGGAGAGGAA CATATTATCT CGGAGCACGA GTCCTTGGAT CCCCCAGTTA 
CAGTACGCCT TTCAGGACAA AAATAACCTT TACCTGGTCA TGGAATATCA GCCTGGAGGG 
GATTTGCTGT CGCTTCTGAA CAGATACGAG GACCAATTAG ATGAGAGCAT GATCCAGTTT 
TACCTTGCTG AGCTGATTTT GGCTGTCCAC AGCGTGCACC AGATGGGATA TGTGCATCGA 
GACATCAAGC CCGAGAACAT CCTCATCGAC CGGACGGGAC ACATCAAGCT GGTGGATTTT 
GGATCAGCCG CTAAGATGAA TTCAAATAAG GTGGATGCCA AACTCCCCAT TGGGACCCCG 
GATTACATGG CTCCGGAAGT GTTGACCGTG ATGAACGAGG ACCGAAGGGG CACATACGGC 
TTGGACTGTG ACTGGTGGTC TGTCGGAGTT GTTGCTTATG AGATGGTTTA TGGGAAGACC 
CCATTCACAG AGGGAACCTC CGCCCGGACC TTCAACAACA TCATGAACTT CCAGCGGTTT 
TTGAAGTTCC CAGATGACCC CAAAGTTAGC AGTGAGCTCC TTGATCTGCT TCAGAGTCTG 
CTGTGTGTCC AGAAAGAGAG ACTGAAGTTC GAGGGTCTCT GCTGCCACCC TTTCTTTGCC 
AGAACGGACT GGAACAACAT CCGTAACTCT CCTCCCCCCT TCGTCCCCAC CCTCAAGTCT 
GACGATGACA CCTCCAATTT TGATGAACCA GAGAAGAATT CGTGGGTTTC ATCCTCTGTG 
TGCCAGCTGA GCCCCTCGGG CTTCTCAGGC GAAGAGCTGC CGTTTGTGGG ATTTTCGTAC 
AGCAAGGCAC TGGGGTATCT TGGTAGATCT GAGTCTGTCG TGTCGAGTCT GGACTCCCCT 
GCCAAGGTTA GCTCCATGGA AAAGAAACTT CTCATCAAAA GCAAAGAGCT CCAAGACTCC 
CAGGACAAGT GTCACAAGAT GGAGCAGGAA ATGACCCGGT TACATCGCAG AGTGTCAGAG 
GTGGAGGCTG TGCTTAGTCA GAAGGAGGTG GAGCTGAAGG CCTCTGAGAC TCAGAGATCC 
CTCCTGGAGC AGGACCTTGC TACCTACATC ACAGAATGCA GTAGCTTAAA GCGAAGTTTG 
GAGCAAGCGC GGATGGAGGT GTCCCAGGAG GATGACAAAG CTCTGCAGCT TCTCCACGAC 
ATCCGAGAGC AGAGCCGGAA GCTCCAGGAG ATCAAGGAGC AGGAGTACCA GGCTCAGGTG 
GAGGAGATGA GGCTGATGAT GAATCAGCTG GAAGAAGACC TGGTGTCAGC CCGCAGACGC 
AGCGATCTCT ACGAGTCTGA GCTGAGGGAG TCTCGGCTTG CCGCCGAGGA ATTCAAGCGG 
AAGGCAAACG AATGTCAGCA CAAACTGATG AAGGTAGTCA GCCACCCTCC AAGAGGGGAC 
TCCGGGGGCA CGGCCCTGGA TGATCTTCAC AAGATGCAAG GCCACGCTGG CTTAACGAGT 
GCTAAGGACC AAGGGAAGCC TGAAGTGGGA GAATATTCCA AACTGGAGAA GATCAATGCT 
GAGCAGCAGC TGAAGATCCA GGAGCTCCAG GAGAAGCTGG AGAAGGCTGT AAAAGCCAGC 
ACAGAGGCCA CCGAGCTCCT GCAGAATATC CGCCAGGCAA AGGAGCGAGC AGAGCGGGAG 
CTAGAGAAGC TACACAACCG GGAAGACTCC TCCGAGGGCA TCAAAAAGAA GCTGGTGGAA 
GCCGAGGAAC GCCGCCACTC CCTGGAGAAC AAGGTAAAGA GACTAGAGAC CATGGAGCGT 
AGAGAGAACA GACTGAAGGA TGACATCCAG ACAAAGTCCG AACAGATCCA GCAGATGGCT 
GATAAAATTC TGGAGCTGGA GGAGAAACAT CGGGAGGCTC AGGTCTCAGC TCAACATCTA 
GAAGTACACT TGAAACAGAA AGAACAGCAC TACGAGGAAA AGATCAAGGT ATTGGACAAT 
CAGATAAAGA AGGACCTGGC CGACAAGGAG AGCCTGGAGA ACATGATGCA GAGACACGAG 
GAGGAGGCCC ACGAGAAGGG CAAGATCCTC AGCGAGCAGA AGGCGATGAT CAACGCGATG 
GATTCCAAGA TCCGATCCCT GGAGCAGAGG ATCGTGGAGC TGTCGGAAGC CAACAAGCTT 
GCGGCAAACA GCAGTCTCTT CACCCAGAGG AACATGAAGG CCCAGGAAGA GATGATCTCA 
GAACTCAGGC AGCAGAAATT TTACCTGGAG ACGCAGGCCG GAAAGCTGGA GGCCCAGAAC 
CGAAAGCTGG AAGAGCAACT GGAGAAGATC AGCCACCAAG ATCACAGTGA CAAGAGTCGG 
CTGCTGGAGC TGGAAACAAG GCTGAGGGAG GTCAGCCTGG AGCACGAGGA GCAGAAGCTG 
GAGCTGAAGC GGCAGCTCAC GGAGCTGCAG CTGTCCCTGC AGGAGCGCGA GTCCCAGCTG 
ACGGCCCTGC AGGCCGCCCG GGCAGCGCTG GAGAGCCAGC TCCGCCAGGC GAAGACAGAG 
CTGGAGGAGA CAACCGCGGA AGCGGAGGAG GAGATCCAGG CGCTCACGGC ACATCGAGAT 
GAGATCCAGC GCAAATTCGA TGCCCTTCGC AACAGCTGCA CGGTCATCAC CGACCTGGAG 
GAGCAGCTGA ACCAGCTCAC CGAGGACAAC GCCGAGCTCA ACAACCAAAA CTTCTACCTG 
TCCAAACAAC TCGATGAGGC TTCCGGGGCC AATGACGAGA TTGTGCAGCT GCGGAGTGAG 
GTGGACCATC TCCGCCGTGA GATCACGGAG CGGGAGATGC AGCTCACCAG CCAGAAGCAA 
GCACAACTCA GTGCTCCTGA TTTGCAGACG ATGGAGGCTC TGAAGACGAC ATGCACCATG 
CTGGAGGAGC AGGTCCTGGA CCTGGAGGCC CTGAACGACG AGCTGCTGGA GAAGGAGCGC 
CAGTGGGAGG CCTGGCGGAG CGTCCTCGGC GACGAGAAGT CCCAGTTCGA GTGTCGAGTT 
CGAGAGCTAC AGAGGATGCT GGACACCGAG AAGCAGAGCA GGGCTAGGGC CGATCAGCGG 
ATCACCGAGT CGCGCCAGGT GGTGGAGTTG GCGGTGAAGG AACACAAGGC CGAGATTCTT 
GCTCTGCAGC AGGCTCTCAA GGAGCAGAAG CTCAAAGCCG AGAGCCTGTC GGACAAGCTC 
AACGACCTGG AGAAGAAGCA CGCCATGCTG GAGATGAACG CCCGGAGCTT ACAGCAGAAA 
CTAGAGACAG AGCGGGAGCT CAAACAGAGG CTTCTGGAGG AGCAAGCCAA ATTGCAGCAG 
CAGATGGACC TGCAGAAGAA CCACATCTTC AGACTGACGC AAGGGCTGCA GGAGGCGCTG 
GACCGGGCGG ATCTGCTGAA GACAGAAAGG AGCGACCTGG AGTACCAGCT GGAAAACATT 
CAGGTTCTCT ACTCTCACGA GAAAGTGAAA ATGGAAGGCA CAATCTCTCA GCAAACCAAA 
CTCATTGATT TCCTGCAAGC CAAAATGGAC CAGCCTGCTA AAAAGAAAAA GGGTTTATTT 
AGTCGACGGA AAGAGGACCC TGCTTTGCCC ACACAGGTTC CTCTGCAGTA CAATGAGCTG 
AAGCTAGCCC TGGAAAAGGA GAAAGCCCGA TGCGCGGAGC TGGAGGAGGC CCTTCAGAAG 
ACCCGCATCG AACTCCGCTC TGCGCGGGAG GAAGCTGCCC ACCGCAAAGC CACGGACCAC 
CCGCACCCGT CTACGCCAGC CACTGCGAGG CAGCAGATCG CCATGTCTGC CATTGTGCGG 
TCGCCCGAGC ACCAGCCCAG TGCCATGAGC CTGCTTGCCC CACCATCCAG CCGCAGAAAG 
GAGTCGTCAA CTCCAGAGGA ATTCAGCCGG CGTCTGAAAG AGCGCATGCA CCACAACATC 
CCTCACCGGT TTAATGTGGG CCTGAACATG AGAGCCACCA AGTGCGCCGT GTGTCTGGAT 
ACTGTGCACT TTGGACGCCA GGCATCCAAA TGCCTCGAAT GTCAAGTGAT GTGTCACCCC 
AAATGCTCCA CCTGCTTGCC CGCTACCTGT GGCCTGCCAG CTGAATATGC CACACACTTC 
ACTGAGGCCT TCTGCCGGGA CAAAATGAAC TCCCCGGGGC TCCAGAGCAA GGAGCCTGGC 
AGCAGCTTGC ACCTGGAAGG GTGGATGAAA GTGCCCAGAA ATAACAAACG GGGACAGCAA 
GGCTGGGACA GGAAGTACAT TGTTCTGGAG GGGTCAAAAG TCCTCATCTA TGACAATGAA 
GCCAGAGAAG CTGGACAGAG GCCGGTGGAA GAATTTGAGC TGTGCCTTCC TGACGGGGAC 
GTATCTATTC ATGGTGCCGT TGGTGCTTCA GAACTTGCAA ATACCGCCAA AGCAGATGTC 
CCATACATCC TGAAGATGGA GTCTCACCCA CACACCACCT GCTGGCCTGG GAGAACCCTC 
TACTTGCTAG CACCCAGCTT CCCCGACAAG CAGCGCTGGG TCACCGCCTT AGAATCTGTC 
GTCGCAGGTG GGAGAGTTTC TAGGGAAAAG GCCGAAGCCG ATGCTGCACG TGACCATACT 
TCGAGTGAGC ATCAGCCAGT GTGGGTTGAG AAATTACTTG GAAACTCTCT GCTGAAACTG 
GAGGGCGATG ACCGGCTTGA CATGAACTGC ACCCTGCCCT TCAGTGACCA GGTAGTGCTG 
GTGGGCACGG AGGAAGGCCT CTACGCCCTG AATGTCTTGA AAAACTCCTT AACCCACATC 
CCAGGGATTG GCGCAGTCTT CCAAATTTAC ATCATCAAGG ACCTGGAGAA GCTGCTCATG 
ATAGCAGGGG AAGAGCGGGC TCTGTGCCTG GTGGACGTGA AGAAGGTGAA GCAGTCCCTG 
GCGCAGTCAC ACCTGCCTGC CCAGCCCGAC GTCTCCCCCA ACATATTCGA AGCCGTCAAA 
GGCTGCCACT TGTTCGCTGC TGGCAAGATC GAGAACAGCC TGTGCATCTG CGCCGCTATG 
CCAAGCAAAG TCGTCATCCT CCGCTACAAT GACAACCTCA GCAAGTACTG CATCCGCAAG 
GAGATCGAGA CCTCAGAGCC CTGCAGCTGT ATCCACTTCA CCAACTACAG CATCCTCATT 
GGGACCAACA AATTCTATGA GATCGACATG AAGCAGTACA CGCTTGACGA GTTCCTGGAC 
AAGAACGACC ATTCCTTGGC ACCTGCTGTG TTCGCCTCCT CGTCCAACAG CTTCCCTGTC 
TCCATTGTGC AGGCGAACAG CGCCGGGCAG CGAGAAGAAT ACCTGCTGTG CTTCCACGAA 
TTTGGGGTGT TCGTGGATTC TTACGGAAGA CGTAGCCGCA CAGATGATCT CAAGTGGAGT 
CGCTTACCTC TGGCCTTCGC CTACAGAGAA CCTTATCTGT TTGTGACTCA CTTCAACTCC 
CTGGAAGTCA TTGAGATCCA GGCCAGATCC TCACTGGGGA GCCCTGCCCG AGCATATCTG 
GAAATTCCAA ACCCTCGCTA CCTGGGCCCC GCGATTTCCT CCGGAGCGAT TTACCTGGCC 
TCCTCATACC AGGACAAGTT AAGGGTCATA TGCTGCAAAG GAAACCTCGT GAAGGAGTCA 
GGCACTGAGC AGCACCGGGT GCCCTCCACC TCCCGCAGAT GTTTCAGGCT GCTGATTCTT 
CGTGGTGGCT GTCAGTTCTC CCATCGATCC AGGGAGAGGC ACGGGCCCAG GGCATGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cit ( XM_006530143.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006530206.1 Mus musculus citron (Cit), transcript variant X14, mRNA.

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CloneID	OMu35783
Clone ID Related Accession (Same CDS sequence)	XM_006530143.3 , XM_006530143.4
Accession Version	XM_006530143.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6087bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	citron Rho-interacting kinase isoform X11
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000071.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006530143.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTGAAGT TCAAGTATGG TGTGCGGAAC CCGCCGGAGG CCAGTGCCTC CGAGCCCATT 
GCCAGTCGGG CCTCCAGGCT AAATCTCTTC TTCCAGGGGA AACCGCCCCT CATGACTCAA 
CAGCAGATGT CTGCTCTTTC CCGGGAAGGG ATGCTAGACG CCCTCTTCGC TCTCTTTGAA 
GAGTGCAGCC AACCCGCCCT GATGAAGATG AAGCACGTGA GCAGCTTTGT CCAGAAGTAT 
TCCGACACCA TAGCCGAGTT GCGGGAGCTG CAGCCGTCGG CGAGAGACTT CGAAGTTCGA 
AGCCTTGTGG GCTGTGGTCA CTTCGCTGAA GTGCAGGTGG TTAGAGAGAA GGCGACCGGG 
GACGTCTATG CCATGAAAAT CATGAAGAAG AAGGCTTTGC TGGCCCAGGA ACAGGTTTCA 
TTTTTCGAGG AGGAGAGGAA CATATTATCT CGGAGCACGA GTCCTTGGAT CCCCCAGTTA 
CAGTACGCCT TTCAGGACAA AAATAACCTT TACCTGGTCA TGGAATATCA GCCTGGAGGG 
GATTTGCTGT CGCTTCTGAA CAGATACGAG GACCAATTAG ATGAGAGCAT GATCCAGTTT 
TACCTTGCTG AGCTGATTTT GGCTGTCCAC AGCGTGCACC AGATGGGATA TGTGCATCGA 
GACATCAAGC CCGAGAACAT CCTCATCGAC CGGACGGGAC ACATCAAGCT GGTGGATTTT 
GGATCAGCCG CTAAGATGAA TTCAAATAAG GTGGATGCCA AACTCCCCAT TGGGACCCCG 
GATTACATGG CTCCGGAAGT GTTGACCGTG ATGAACGAGG ACCGAAGGGG CACATACGGC 
TTGGACTGTG ACTGGTGGTC TGTCGGAGTT GTTGCTTATG AGATGGTTTA TGGGAAGACC 
CCATTCACAG AGGGAACCTC CGCCCGGACC TTCAACAACA TCATGAACTT CCAGCGGTTT 
TTGAAGTTCC CAGATGACCC CAAAGTTAGC AGTGAGCTCC TTGATCTGCT TCAGAGTCTG 
CTGTGTGTCC AGAAAGAGAG ACTGAAGTTC GAGGGTCTCT GCTGCCACCC TTTCTTTGCC 
AGAACGGACT GGAACAACAT CCGTAACTCT CCTCCCCCCT TCGTCCCCAC CCTCAAGTCT 
GACGATGACA CCTCCAATTT TGATGAACCA GAGAAGAATT CGTGGGTTTC ATCCTCTGTG 
TGCCAGCTGA GCCCCTCGGG CTTCTCAGGC GAAGAGCTGC CGTTTGTGGG ATTTTCGTAC 
AGCAAGGCAC TGGGGTATCT TGGTAGATCT GAGTCTGTCG TGTCGAGTCT GGACTCCCCT 
GCCAAGGTTA GCTCCATGGA AAAGAAACTT CTCATCAAAA GCAAAGAGCT CCAAGACTCC 
CAGGACAAGT GTCACAAGAT GGAGCAGGAA ATGACCCGGT TACATCGCAG AGTGTCAGAG 
GTGGAGGCTG TGCTTAGTCA GAAGGAGGTG GAGCTGAAGG CCTCTGAGAC TCAGAGATCC 
CTCCTGGAGC AGGACCTTGC TACCTACATC ACAGAATGCA GTAGCTTAAA GCGAAGTTTG 
GAGCAAGCGC GGATGGAGGT GTCCCAGGAG GATGACAAAG CTCTGCAGCT TCTCCACGAC 
ATCCGAGAGC AGAGCCGGAA GCTCCAGGAG ATCAAGGAGC AGGAGTACCA GGCTCAGGTG 
GAGGAGATGA GGCTGATGAT GAATCAGCTG GAAGAAGACC TGGTGTCAGC CCGCAGACGC 
AGCGATCTCT ACGAGTCTGA GCTGAGGGAG TCTCGGCTTG CCGCCGAGGA ATTCAAGCGG 
AAGGCAAACG AATGTCAGCA CAAACTGATG AAGGCTAAGG ACCAAGGGAA GCCTGAAGTG 
GGAGAATATT CCAAACTGGA GAAGATCAAT GCTGAGCAGC AGCTGAAGAT CCAGGAGCTC 
CAGGAGAAGC TGGAGAAGGC TGTAAAAGCC AGCACAGAGG CCACCGAGCT CCTGCAGAAT 
ATCCGCCAGG CAAAGGAGCG AGCAGAGCGG GAGCTAGAGA AGCTACACAA CCGGGAAGAC 
TCCTCCGAGG GCATCAAAAA GAAGCTGGTG GAAGCCGAGG AGCTGGAGGA GAAACATCGG 
GAGGCTCAGG TCTCAGCTCA ACATCTAGAA GTACACTTGA AACAGAAAGA ACAGCACTAC 
GAGGAAAAGA TCAAGGTATT GGACAATCAG ATAAAGAAGG ACCTGGCCGA CAAGGAGAGC 
CTGGAGAACA TGATGCAGAG ACACGAGGAG GAGGCCCACG AGAAGGGCAA GATCCTCAGC 
GAGCAGAAGG CGATGATCAA CGCGATGGAT TCCAAGATCC GATCCCTGGA GCAGAGGATC 
GTGGAGCTGT CGGAAGCCAA CAAGCTTGCG GCAAACAGCA GTCTCTTCAC CCAGAGGAAC 
ATGAAGGCCC AGGAAGAGAT GATCTCAGAA CTCAGGCAGC AGAAATTTTA CCTGGAGACG 
CAGGCCGGAA AGCTGGAGGC CCAGAACCGA AAGCTGGAAG AGCAACTGGA GAAGATCAGC 
CACCAAGATC ACAGTGACAA GAGTCGGCTG CTGGAGCTGG AAACAAGGCT GAGGGAGGTC 
AGCCTGGAGC ACGAGGAGCA GAAGCTGGAG CTGAAGCGGC AGCTCACGGA GCTGCAGCTG 
TCCCTGCAGG AGCGCGAGTC CCAGCTGACG GCCCTGCAGG CCGCCCGGGC AGCGCTGGAG 
AGCCAGCTCC GCCAGGCGAA GACAGAGCTG GAGGAGACAA CCGCGGAAGC GGAGGAGGAG 
ATCCAGGCGC TCACGGCACA TCGAGATGAG ATCCAGCGCA AATTCGATGC CCTTCGCAAC 
AGCTGCACGG TCATCACCGA CCTGGAGGAG CAGCTGAACC AGCTCACCGA GGACAACGCC 
GAGCTCAACA ACCAAAACTT CTACCTGTCC AAACAACTCG ATGAGGCTTC CGGGGCCAAT 
GACGAGATTG TGCAGCTGCG GAGTGAGGTG GACCATCTCC GCCGTGAGAT CACGGAGCGG 
GAGATGCAGC TCACCAGCCA GAAGCAAACG ATGGAGGCTC TGAAGACGAC ATGCACCATG 
CTGGAGGAGC AGGTCCTGGA CCTGGAGGCC CTGAACGACG AGCTGCTGGA GAAGGAGCGC 
CAGTGGGAGG CCTGGCGGAG CGTCCTCGGC GACGAGAAGT CCCAGTTCGA GTGTCGAGTT 
CGAGAGCTAC AGAGGATGCT GGACACCGAG AAGCAGAGCA GGGCTAGGGC CGATCAGCGG 
ATCACCGAGT CGCGCCAGGT GGTGGAGTTG GCGGTGAAGG AACACAAGGC CGAGATTCTT 
GCTCTGCAGC AGGCTCTCAA GGAGCAGAAG CTCAAAGCCG AGAGCCTGTC GGACAAGCTC 
AACGACCTGG AGAAGAAGCA CGCCATGCTG GAGATGAACG CCCGGAGCTT ACAGCAGAAA 
CTAGAGACAG AGCGGGAGCT CAAACAGAGG CTTCTGGAGG AGCAAGCCAA ATTGCAGCAG 
CAGATGGACC TGCAGAAGAA CCACATCTTC AGACTGACGC AAGGGCTGCA GGAGGCGCTG 
GACCGGGCGG ATCTGCTGAA GACAGAAAGG AGCGACCTGG AGTACCAGCT GGAAAACATT 
CAGGTTCTCT ACTCTCACGA GAAAGTGAAA ATGGAAGGCA CAATCTCTCA GCAAACCAAA 
CTCATTGATT TCCTGCAAGC CAAAATGGAC CAGCCTGCTA AAAAGAAAAA GGGTTTATTT 
AGTCGACGGA AAGAGGACCC TGCTTTGCCC ACACAGGTTC CTCTGCAGTA CAATGAGCTG 
AAGCTAGCCC TGGAAAAGGA GAAAGCCCGA TGCGCGGAGC TGGAGGAGGC CCTTCAGAAG 
ACCCGCATCG AACTCCGCTC TGCGCGGGAG GAAGCTGCCC ACCGCAAAGC CACGGACCAC 
CCGCACCCGT CTACGCCAGC CACTGCGAGG CAGCAGATCG CCATGTCTGC CATTGTGCGG 
TCGCCCGAGC ACCAGCCCAG TGCCATGAGC CTGCTTGCCC CACCATCCAG CCGCAGAAAG 
GAGTCGTCAA CTCCAGAGGA ATTCAGCCGG CGTCTGAAAG AGCGCATGCA CCACAACATC 
CCTCACCGGT TTAATGTGGG CCTGAACATG AGAGCCACCA AGTGCGCCGT GTGTCTGGAT 
ACTGTGCACT TTGGACGCCA GGCATCCAAA TGCCTCGAAT GTCAAGTGAT GTGTCACCCC 
AAATGCTCCA CCTGCTTGCC CGCTACCTGT GGCCTGCCAG CTGAATATGC CACACACTTC 
ACTGAGGCCT TCTGCCGGGA CAAAATGAAC TCCCCGGGGC TCCAGAGCAA GGAGCCTGGC 
AGCAGCTTGC ACCTGGAAGG GTGGATGAAA GTGCCCAGAA ATAACAAACG GGGACAGCAA 
GGCTGGGACA GGAAGTACAT TGTTCTGGAG GGGTCAAAAG TCCTCATCTA TGACAATGAA 
GCCAGAGAAG CTGGACAGAG GCCGGTGGAA GAATTTGAGC TGTGCCTTCC TGACGGGGAC 
GTATCTATTC ATGGTGCCGT TGGTGCTTCA GAACTTGCAA ATACCGCCAA AGCAGATGTC 
CCATACATCC TGAAGATGGA GTCTCACCCA CACACCACCT GCTGGCCTGG GAGAACCCTC 
TACTTGCTAG CACCCAGCTT CCCCGACAAG CAGCGCTGGG TCACCGCCTT AGAATCTGTC 
GTCGCAGGTG GGAGAGTTTC TAGGGAAAAG GCCGAAGCCG ATGCTAAATT ACTTGGAAAC 
TCTCTGCTGA AACTGGAGGG CGATGACCGG CTTGACATGA ACTGCACCCT GCCCTTCAGT 
GACCAGGTAG TGCTGGTGGG CACGGAGGAA GGCCTCTACG CCCTGAATGT CTTGAAAAAC 
TCCTTAACCC ACATCCCAGG GATTGGCGCA GTCTTCCAAA TTTACATCAT CAAGGACCTG 
GAGAAGCTGC TCATGATAGC AGGGGAAGAG CGGGCTCTGT GCCTGGTGGA CGTGAAGAAG 
GTGAAGCAGT CCCTGGCGCA GTCACACCTG CCTGCCCAGC CCGACGTCTC CCCCAACATA 
TTCGAAGCCG TCAAAGGCTG CCACTTGTTC GCTGCTGGCA AGATCGAGAA CAGCCTGTGC 
ATCTGCGCCG CTATGCCAAG CAAAGTCGTC ATCCTCCGCT ACAATGACAA CCTCAGCAAG 
TACTGCATCC GCAAGGAGAT CGAGACCTCA GAGCCCTGCA GCTGTATCCA CTTCACCAAC 
TACAGCATCC TCATTGGGAC CAACAAATTC TATGAGATCG ACATGAAGCA GTACACGCTT 
GACGAGTTCC TGGACAAGAA CGACCATTCC TTGGCACCTG CTGTGTTCGC CTCCTCGTCC 
AACAGCTTCC CTGTCTCCAT TGTGCAGGCG AACAGCGCCG GGCAGCGAGA AGAATACCTG 
CTGTGCTTCC ACGAATTTGG GGTGTTCGTG GATTCTTACG GAAGACGTAG CCGCACAGAT 
GATCTCAAGT GGAGTCGCTT ACCTCTGGCC TTCGCCTACA GAGAACCTTA TCTGTTTGTG 
ACTCACTTCA ACTCCCTGGA AGTCATTGAG ATCCAGGCCA GATCCTCACT GGGGAGCCCT 
GCCCGAGCAT ATCTGGAAAT TCCAAACCCT CGCTACCTGG GCCCCGCGAT TTCCTCCGGA 
GCGATTTACC TGGCCTCCTC ATACCAGGAC AAGTTAAGGG TCATATGCTG CAAAGGAAAC 
CTCGTGAAGG AGTCAGGCAC TGAGCAGCAC CGGGTGCCCT CCACCTCCCG CAGCAGCCCC 
AACAAGCGAG GCCCACCAAC ATACAACGAG CACATCACCA AACGCGTGGC CTCCAGCCCG 
GCGCCACCGG AAGGCCCCAG CCACCCCCGA GAGCCAAGCA CACCGCACCG CTACCGAGAC 
AGAGAGGGTC GGACAGAGCT GCGCAGGGAC AAGTCTCCAG GCCGCCCTCT GGAGCGGGAG 
AAGTCCCCAG GCCGAATGCT CAGCACTAGG AGAGAGCGGT CCCCAGGGAG ACTGTTTGAA 
GACAGCAGCA GGGGCCGGCT GCCTGCAGGA GCAGTGAGGA CCCCACTGTC CCAGGTTAAC 
AAGGTCTGGG ACCAGTCTTC AGTATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006530206.1
CDS	157..6243
Translation	MLKFKYGVRNPPEASASEPIASRASRLNLFFQGKPPLMTQQQMSALSREGMLDALFALFEECSQPALMKMKHVSSFVQKYSDTIAELRELQPSARDFEVRSLVGCGHFAEVQVVREKATGDVYAMKIMKKKALLAQEQVSFFEEERNILSRSTSPWIPQLQYAFQDKNNLYLVMEYQPGGDLLSLLNRYEDQLDESMIQFYLAELILAVHSVHQMGYVHRDIKPENILIDRTGHIKLVDFGSAAKMNSNKVDAKLPIGTPDYMAPEVLTVMNEDRRGTYGLDCDWWSVGVVAYEMVYGKTPFTEGTSARTFNNIMNFQRFLKFPDDPKVSSELLDLLQSLLCVQKERLKFEGLCCHPFFARTDWNNIRNSPPPFVPTLKSDDDTSNFDEPEKNSWVSSSVCQLSPSGFSGEELPFVGFSYSKALGYLGRSESVVSSLDSPAKVSSMEKKLLIKSKELQDSQDKCHKMEQEMTRLHRRVSEVEAVLSQKEVELKASETQRSLLEQDLATYITECSSLKRSLEQARMEVSQEDDKALQLLHDIREQSRKLQEIKEQEYQAQVEEMRLMMNQLEEDLVSARRRSDLYESELRESRLAAEEFKRKANECQHKLMKAKDQGKPEVGEYSKLEKINAEQQLKIQELQEKLEKAVKASTEATELLQNIRQAKERAERELEKLHNREDSSEGIKKKLVEAEELEEKHREAQVSAQHLEVHLKQKEQHYEEKIKVLDNQIKKDLADKESLENMMQRHEEEAHEKGKILSEQKAMINAMDSKIRSLEQRIVELSEANKLAANSSLFTQRNMKAQEEMISELRQQKFYLETQAGKLEAQNRKLEEQLEKISHQDHSDKSRLLELETRLREVSLEHEEQKLELKRQLTELQLSLQERESQLTALQAARAALESQLRQAKTELEETTAEAEEEIQALTAHRDEIQRKFDALRNSCTVITDLEEQLNQLTEDNAELNNQNFYLSKQLDEASGANDEIVQLRSEVDHLRREITEREMQLTSQKQTMEALKTTCTMLEEQVLDLEALNDELLEKERQWEAWRSVLGDEKSQFECRVRELQRMLDTEKQSRARADQRITESRQVVELAVKEHKAEILALQQALKEQKLKAESLSDKLNDLEKKHAMLEMNARSLQQKLETERELKQRLLEEQAKLQQQMDLQKNHIFRLTQGLQEALDRADLLKTERSDLEYQLENIQVLYSHEKVKMEGTISQQTKLIDFLQAKMDQPAKKKKGLFSRRKEDPALPTQVPLQYNELKLALEKEKARCAELEEALQKTRIELRSAREEAAHRKATDHPHPSTPATARQQIAMSAIVRSPEHQPSAMSLLAPPSSRRKESSTPEEFSRRLKERMHHNIPHRFNVGLNMRATKCAVCLDTVHFGRQASKCLECQVMCHPKCSTCLPATCGLPAEYATHFTEAFCRDKMNSPGLQSKEPGSSLHLEGWMKVPRNNKRGQQGWDRKYIVLEGSKVLIYDNEAREAGQRPVEEFELCLPDGDVSIHGAVGASELANTAKADVPYILKMESHPHTTCWPGRTLYLLAPSFPDKQRWVTALESVVAGGRVSREKAEADAKLLGNSLLKLEGDDRLDMNCTLPFSDQVVLVGTEEGLYALNVLKNSLTHIPGIGAVFQIYIIKDLEKLLMIAGEERALCLVDVKKVKQSLAQSHLPAQPDVSPNIFEAVKGCHLFAAGKIENSLCICAAMPSKVVILRYNDNLSKYCIRKEIETSEPCSCIHFTNYSILIGTNKFYEIDMKQYTLDEFLDKNDHSLAPAVFASSSNSFPVSIVQANSAGQREEYLLCFHEFGVFVDSYGRRSRTDDLKWSRLPLAFAYREPYLFVTHFNSLEVIEIQARSSLGSPARAYLEIPNPRYLGPAISSGAIYLASSYQDKLRVICCKGNLVKESGTEQHRVPSTSRSSPNKRGPPTYNEHITKRVASSPAPPEGPSHPREPSTPHRYRDREGRTELRRDKSPGRPLEREKSPGRMLSTRRERSPGRLFEDSSRGRLPAGAVRTPLSQVNKVWDQSSV

Target ORF information:

RefSeq Version	XM_006530143.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus citron (Cit), transcript variant X14, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006530143.3

ORF Insert Sequence:

ATGTTGAAGT TCAAGTATGG TGTGCGGAAC CCGCCGGAGG CCAGTGCCTC CGAGCCCATT 
GCCAGTCGGG CCTCCAGGCT AAATCTCTTC TTCCAGGGGA AACCGCCCCT CATGACTCAA 
CAGCAGATGT CTGCTCTTTC CCGGGAAGGG ATGCTAGACG CCCTCTTCGC TCTCTTTGAA 
GAGTGCAGCC AACCCGCCCT GATGAAGATG AAGCACGTGA GCAGCTTTGT CCAGAAGTAT 
TCCGACACCA TAGCCGAGTT GCGGGAGCTG CAGCCGTCGG CGAGAGACTT CGAAGTTCGA 
AGCCTTGTGG GCTGTGGTCA CTTCGCTGAA GTGCAGGTGG TTAGAGAGAA GGCGACCGGG 
GACGTCTATG CCATGAAAAT CATGAAGAAG AAGGCTTTGC TGGCCCAGGA ACAGGTTTCA 
TTTTTCGAGG AGGAGAGGAA CATATTATCT CGGAGCACGA GTCCTTGGAT CCCCCAGTTA 
CAGTACGCCT TTCAGGACAA AAATAACCTT TACCTGGTCA TGGAATATCA GCCTGGAGGG 
GATTTGCTGT CGCTTCTGAA CAGATACGAG GACCAATTAG ATGAGAGCAT GATCCAGTTT 
TACCTTGCTG AGCTGATTTT GGCTGTCCAC AGCGTGCACC AGATGGGATA TGTGCATCGA 
GACATCAAGC CCGAGAACAT CCTCATCGAC CGGACGGGAC ACATCAAGCT GGTGGATTTT 
GGATCAGCCG CTAAGATGAA TTCAAATAAG GTGGATGCCA AACTCCCCAT TGGGACCCCG 
GATTACATGG CTCCGGAAGT GTTGACCGTG ATGAACGAGG ACCGAAGGGG CACATACGGC 
TTGGACTGTG ACTGGTGGTC TGTCGGAGTT GTTGCTTATG AGATGGTTTA TGGGAAGACC 
CCATTCACAG AGGGAACCTC CGCCCGGACC TTCAACAACA TCATGAACTT CCAGCGGTTT 
TTGAAGTTCC CAGATGACCC CAAAGTTAGC AGTGAGCTCC TTGATCTGCT TCAGAGTCTG 
CTGTGTGTCC AGAAAGAGAG ACTGAAGTTC GAGGGTCTCT GCTGCCACCC TTTCTTTGCC 
AGAACGGACT GGAACAACAT CCGTAACTCT CCTCCCCCCT TCGTCCCCAC CCTCAAGTCT 
GACGATGACA CCTCCAATTT TGATGAACCA GAGAAGAATT CGTGGGTTTC ATCCTCTGTG 
TGCCAGCTGA GCCCCTCGGG CTTCTCAGGC GAAGAGCTGC CGTTTGTGGG ATTTTCGTAC 
AGCAAGGCAC TGGGGTATCT TGGTAGATCT GAGTCTGTCG TGTCGAGTCT GGACTCCCCT 
GCCAAGGTTA GCTCCATGGA AAAGAAACTT CTCATCAAAA GCAAAGAGCT CCAAGACTCC 
CAGGACAAGT GTCACAAGAT GGAGCAGGAA ATGACCCGGT TACATCGCAG AGTGTCAGAG 
GTGGAGGCTG TGCTTAGTCA GAAGGAGGTG GAGCTGAAGG CCTCTGAGAC TCAGAGATCC 
CTCCTGGAGC AGGACCTTGC TACCTACATC ACAGAATGCA GTAGCTTAAA GCGAAGTTTG 
GAGCAAGCGC GGATGGAGGT GTCCCAGGAG GATGACAAAG CTCTGCAGCT TCTCCACGAC 
ATCCGAGAGC AGAGCCGGAA GCTCCAGGAG ATCAAGGAGC AGGAGTACCA GGCTCAGGTG 
GAGGAGATGA GGCTGATGAT GAATCAGCTG GAAGAAGACC TGGTGTCAGC CCGCAGACGC 
AGCGATCTCT ACGAGTCTGA GCTGAGGGAG TCTCGGCTTG CCGCCGAGGA ATTCAAGCGG 
AAGGCAAACG AATGTCAGCA CAAACTGATG AAGGCTAAGG ACCAAGGGAA GCCTGAAGTG 
GGAGAATATT CCAAACTGGA GAAGATCAAT GCTGAGCAGC AGCTGAAGAT CCAGGAGCTC 
CAGGAGAAGC TGGAGAAGGC TGTAAAAGCC AGCACAGAGG CCACCGAGCT CCTGCAGAAT 
ATCCGCCAGG CAAAGGAGCG AGCAGAGCGG GAGCTAGAGA AGCTACACAA CCGGGAAGAC 
TCCTCCGAGG GCATCAAAAA GAAGCTGGTG GAAGCCGAGG AGCTGGAGGA GAAACATCGG 
GAGGCTCAGG TCTCAGCTCA ACATCTAGAA GTACACTTGA AACAGAAAGA ACAGCACTAC 
GAGGAAAAGA TCAAGGTATT GGACAATCAG ATAAAGAAGG ACCTGGCCGA CAAGGAGAGC 
CTGGAGAACA TGATGCAGAG ACACGAGGAG GAGGCCCACG AGAAGGGCAA GATCCTCAGC 
GAGCAGAAGG CGATGATCAA CGCGATGGAT TCCAAGATCC GATCCCTGGA GCAGAGGATC 
GTGGAGCTGT CGGAAGCCAA CAAGCTTGCG GCAAACAGCA GTCTCTTCAC CCAGAGGAAC 
ATGAAGGCCC AGGAAGAGAT GATCTCAGAA CTCAGGCAGC AGAAATTTTA CCTGGAGACG 
CAGGCCGGAA AGCTGGAGGC CCAGAACCGA AAGCTGGAAG AGCAACTGGA GAAGATCAGC 
CACCAAGATC ACAGTGACAA GAGTCGGCTG CTGGAGCTGG AAACAAGGCT GAGGGAGGTC 
AGCCTGGAGC ACGAGGAGCA GAAGCTGGAG CTGAAGCGGC AGCTCACGGA GCTGCAGCTG 
TCCCTGCAGG AGCGCGAGTC CCAGCTGACG GCCCTGCAGG CCGCCCGGGC AGCGCTGGAG 
AGCCAGCTCC GCCAGGCGAA GACAGAGCTG GAGGAGACAA CCGCGGAAGC GGAGGAGGAG 
ATCCAGGCGC TCACGGCACA TCGAGATGAG ATCCAGCGCA AATTCGATGC CCTTCGCAAC 
AGCTGCACGG TCATCACCGA CCTGGAGGAG CAGCTGAACC AGCTCACCGA GGACAACGCC 
GAGCTCAACA ACCAAAACTT CTACCTGTCC AAACAACTCG ATGAGGCTTC CGGGGCCAAT 
GACGAGATTG TGCAGCTGCG GAGTGAGGTG GACCATCTCC GCCGTGAGAT CACGGAGCGG 
GAGATGCAGC TCACCAGCCA GAAGCAAACG ATGGAGGCTC TGAAGACGAC ATGCACCATG 
CTGGAGGAGC AGGTCCTGGA CCTGGAGGCC CTGAACGACG AGCTGCTGGA GAAGGAGCGC 
CAGTGGGAGG CCTGGCGGAG CGTCCTCGGC GACGAGAAGT CCCAGTTCGA GTGTCGAGTT 
CGAGAGCTAC AGAGGATGCT GGACACCGAG AAGCAGAGCA GGGCTAGGGC CGATCAGCGG 
ATCACCGAGT CGCGCCAGGT GGTGGAGTTG GCGGTGAAGG AACACAAGGC CGAGATTCTT 
GCTCTGCAGC AGGCTCTCAA GGAGCAGAAG CTCAAAGCCG AGAGCCTGTC GGACAAGCTC 
AACGACCTGG AGAAGAAGCA CGCCATGCTG GAGATGAACG CCCGGAGCTT ACAGCAGAAA 
CTAGAGACAG AGCGGGAGCT CAAACAGAGG CTTCTGGAGG AGCAAGCCAA ATTGCAGCAG 
CAGATGGACC TGCAGAAGAA CCACATCTTC AGACTGACGC AAGGGCTGCA GGAGGCGCTG 
GACCGGGCGG ATCTGCTGAA GACAGAAAGG AGCGACCTGG AGTACCAGCT GGAAAACATT 
CAGGTTCTCT ACTCTCACGA GAAAGTGAAA ATGGAAGGCA CAATCTCTCA GCAAACCAAA 
CTCATTGATT TCCTGCAAGC CAAAATGGAC CAGCCTGCTA AAAAGAAAAA GGGTTTATTT 
AGTCGACGGA AAGAGGACCC TGCTTTGCCC ACACAGGTTC CTCTGCAGTA CAATGAGCTG 
AAGCTAGCCC TGGAAAAGGA GAAAGCCCGA TGCGCGGAGC TGGAGGAGGC CCTTCAGAAG 
ACCCGCATCG AACTCCGCTC TGCGCGGGAG GAAGCTGCCC ACCGCAAAGC CACGGACCAC 
CCGCACCCGT CTACGCCAGC CACTGCGAGG CAGCAGATCG CCATGTCTGC CATTGTGCGG 
TCGCCCGAGC ACCAGCCCAG TGCCATGAGC CTGCTTGCCC CACCATCCAG CCGCAGAAAG 
GAGTCGTCAA CTCCAGAGGA ATTCAGCCGG CGTCTGAAAG AGCGCATGCA CCACAACATC 
CCTCACCGGT TTAATGTGGG CCTGAACATG AGAGCCACCA AGTGCGCCGT GTGTCTGGAT 
ACTGTGCACT TTGGACGCCA GGCATCCAAA TGCCTCGAAT GTCAAGTGAT GTGTCACCCC 
AAATGCTCCA CCTGCTTGCC CGCTACCTGT GGCCTGCCAG CTGAATATGC CACACACTTC 
ACTGAGGCCT TCTGCCGGGA CAAAATGAAC TCCCCGGGGC TCCAGAGCAA GGAGCCTGGC 
AGCAGCTTGC ACCTGGAAGG GTGGATGAAA GTGCCCAGAA ATAACAAACG GGGACAGCAA 
GGCTGGGACA GGAAGTACAT TGTTCTGGAG GGGTCAAAAG TCCTCATCTA TGACAATGAA 
GCCAGAGAAG CTGGACAGAG GCCGGTGGAA GAATTTGAGC TGTGCCTTCC TGACGGGGAC 
GTATCTATTC ATGGTGCCGT TGGTGCTTCA GAACTTGCAA ATACCGCCAA AGCAGATGTC 
CCATACATCC TGAAGATGGA GTCTCACCCA CACACCACCT GCTGGCCTGG GAGAACCCTC 
TACTTGCTAG CACCCAGCTT CCCCGACAAG CAGCGCTGGG TCACCGCCTT AGAATCTGTC 
GTCGCAGGTG GGAGAGTTTC TAGGGAAAAG GCCGAAGCCG ATGCTAAATT ACTTGGAAAC 
TCTCTGCTGA AACTGGAGGG CGATGACCGG CTTGACATGA ACTGCACCCT GCCCTTCAGT 
GACCAGGTAG TGCTGGTGGG CACGGAGGAA GGCCTCTACG CCCTGAATGT CTTGAAAAAC 
TCCTTAACCC ACATCCCAGG GATTGGCGCA GTCTTCCAAA TTTACATCAT CAAGGACCTG 
GAGAAGCTGC TCATGATAGC AGGGGAAGAG CGGGCTCTGT GCCTGGTGGA CGTGAAGAAG 
GTGAAGCAGT CCCTGGCGCA GTCACACCTG CCTGCCCAGC CCGACGTCTC CCCCAACATA 
TTCGAAGCCG TCAAAGGCTG CCACTTGTTC GCTGCTGGCA AGATCGAGAA CAGCCTGTGC 
ATCTGCGCCG CTATGCCAAG CAAAGTCGTC ATCCTCCGCT ACAATGACAA CCTCAGCAAG 
TACTGCATCC GCAAGGAGAT CGAGACCTCA GAGCCCTGCA GCTGTATCCA CTTCACCAAC 
TACAGCATCC TCATTGGGAC CAACAAATTC TATGAGATCG ACATGAAGCA GTACACGCTT 
GACGAGTTCC TGGACAAGAA CGACCATTCC TTGGCACCTG CTGTGTTCGC CTCCTCGTCC 
AACAGCTTCC CTGTCTCCAT TGTGCAGGCG AACAGCGCCG GGCAGCGAGA AGAATACCTG 
CTGTGCTTCC ACGAATTTGG GGTGTTCGTG GATTCTTACG GAAGACGTAG CCGCACAGAT 
GATCTCAAGT GGAGTCGCTT ACCTCTGGCC TTCGCCTACA GAGAACCTTA TCTGTTTGTG 
ACTCACTTCA ACTCCCTGGA AGTCATTGAG ATCCAGGCCA GATCCTCACT GGGGAGCCCT 
GCCCGAGCAT ATCTGGAAAT TCCAAACCCT CGCTACCTGG GCCCCGCGAT TTCCTCCGGA 
GCGATTTACC TGGCCTCCTC ATACCAGGAC AAGTTAAGGG TCATATGCTG CAAAGGAAAC 
CTCGTGAAGG AGTCAGGCAC TGAGCAGCAC CGGGTGCCCT CCACCTCCCG CAGCAGCCCC 
AACAAGCGAG GCCCACCAAC ATACAACGAG CACATCACCA AACGCGTGGC CTCCAGCCCG 
GCGCCACCGG AAGGCCCCAG CCACCCCCGA GAGCCAAGCA CACCGCACCG CTACCGAGAC 
AGAGAGGGTC GGACAGAGCT GCGCAGGGAC AAGTCTCCAG GCCGCCCTCT GGAGCGGGAG 
AAGTCCCCAG GCCGAATGCT CAGCACTAGG AGAGAGCGGT CCCCAGGGAG ACTGTTTGAA 
GACAGCAGCA GGGGCCGGCT GCCTGCAGGA GCAGTGAGGA CCCCACTGTC CCAGGTTAAC 
AAGGTCTGGG ACCAGTCTTC AGTATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cit ( XM_006530145.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006530208.1 Mus musculus citron (Cit), transcript variant X16, mRNA.

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CloneID	OMu35785
Clone ID Related Accession (Same CDS sequence)	XM_006530145.3 , XM_006530145.4
Accession Version	XM_006530145.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6042bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	citron Rho-interacting kinase isoform X13
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000071.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006530145.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTGAAGT TCAAGTATGG TGTGCGGAAC CCGCCGGAGG CCAGTGCCTC CGAGCCCATT 
GCCAGTCGGG CCTCCAGGCT AAATCTCTTC TTCCAGGGGA AACCGCCCCT CATGACTCAA 
CAGCAGATGT CTGCTCTTTC CCGGGAAGGG ATGCTAGACG CCCTCTTCGC TCTCTTTGAA 
GAGTGCAGCC AACCCGCCCT GATGAAGATG AAGCACGTGA GCAGCTTTGT CCAGAAGTAT 
TCCGACACCA TAGCCGAGTT GCGGGAGCTG CAGCCGTCGG CGAGAGACTT CGAAGTTCGA 
AGCCTTGTGG GCTGTGGTCA CTTCGCTGAA GTGCAGGTGG TTAGAGAGAA GGCGACCGGG 
GACGTCTATG CCATGAAAAT CATGAAGAAG AAGGCTTTGC TGGCCCAGGA ACAGGTTTCA 
TTTTTCGAGG AGGAGAGGAA CATATTATCT CGGAGCACGA GTCCTTGGAT CCCCCAGTTA 
CAGTACGCCT TTCAGGACAA AAATAACCTT TACCTGGTCA TGGAATATCA GCCTGGAGGG 
GATTTGCTGT CGCTTCTGAA CAGATACGAG GACCAATTAG ATGAGAGCAT GATCCAGTTT 
TACCTTGCTG AGCTGATTTT GGCTGTCCAC AGCGTGCACC AGATGGGATA TGTGCATCGA 
GACATCAAGC CCGAGAACAT CCTCATCGAC CGGACGGGAC ACATCAAGCT GGTGGATTTT 
GGATCAGCCG CTAAGATGAA TTCAAATAAG GTGGATGCCA AACTCCCCAT TGGGACCCCG 
GATTACATGG CTCCGGAAGT GTTGACCGTG ATGAACGAGG ACCGAAGGGG CACATACGGC 
TTGGACTGTG ACTGGTGGTC TGTCGGAGTT GTTGCTTATG AGATGGTTTA TGGGAAGACC 
CCATTCACAG AGGGAACCTC CGCCCGGACC TTCAACAACA TCATGAACTT CCAGCGGTTT 
TTGAAGTTCC CAGATGACCC CAAAGTTAGC AGTGAGCTCC TTGATCTGCT TCAGAGTCTG 
CTGTGTGTCC AGAAAGAGAG ACTGAAGTTC GAGGGTCTCT GCTGCCACCC TTTCTTTGCC 
AGAACGGACT GGAACAACAT CCGTAACTCT CCTCCCCCCT TCGTCCCCAC CCTCAAGTCT 
GACGATGACA CCTCCAATTT TGATGAACCA GAGAAGAATT CGTGGGTTTC ATCCTCTGTG 
TGCCAGCTGA GCCCCTCGGG CTTCTCAGGC GAAGAGCTGC CGTTTGTGGG ATTTTCGTAC 
AGCAAGGCAC TGGGGTATCT TGGTAGATCT GAGTCTGTCG TGTCGAGTCT GGACTCCCCT 
GCCAAGGTTA GCTCCATGGA AAAGAAACTT CTCATCAAAA GCAAAGAGCT CCAAGACTCC 
CAGGACAAGT GTCACAAGAT GGAGCAGGAA ATGACCCGGT TACATCGCAG AGTGTCAGAG 
GTGGAGGCTG TGCTTAGTCA GAAGGAGGTG GAGCTGAAGG CCTCTGAGAC TCAGAGATCC 
CTCCTGGAGC AGGACCTTGC TACCTACATC ACAGAATGCA GTAGCTTAAA GCGAAGTTTG 
GAGCAAGCGC GGATGGAGGT GTCCCAGGAG GATGACAAAG CTCTGCAGCT TCTCCACGAC 
ATCCGAGAGC AGAGCCGGAA GCTCCAGGAG ATCAAGGAGC AGGAGTACCA GGCTCAGGTG 
GAGGAGATGA GGCTGATGAT GAATCAGCTG GAAGAAGACC TGGTGTCAGC CCGCAGACGC 
AGCGATCTCT ACGAGTCTGA GCTGAGGGAG TCTCGGCTTG CCGCCGAGGA ATTCAAGCGG 
AAGGCAAACG AATGTCAGCA CAAACTGATG AAGGCTAAGG ACCAAGGGAA GCCTGAAGTG 
GGAGAATATT CCAAACTGGA GAAGATCAAT GCTGAGCAGC AGCTGAAGAT CCAGGAGCTC 
CAGGAGAAGC TGGAGAAGGC TGTAAAAGCC AGCACAGAGG CCACCGAGCT CCTGCAGAAT 
ATCCGCCAGG CAAAGGAGCG AGCAGAGCGG GAGCTAGAGA AGCTACACAA CCGGGAAGAC 
TCCTCCGAGG GCATCAAAAA GAAGCTGGTG GAAGCCGAGG AGCTGGAGGA GAAACATCGG 
GAGGCTCAGG TCTCAGCTCA ACATCTAGAA GTACACTTGA AACAGAAAGA ACAGCACTAC 
GAGGAAAAGA TCAAGGTATT GGACAATCAG ATAAAGAAGG ACCTGGCCGA CAAGGAGAGC 
CTGGAGAACA TGATGCAGAG ACACGAGGAG GAGGCCCACG AGAAGGGCAA GATCCTCAGC 
GAGCAGAAGG CGATGATCAA CGCGATGGAT TCCAAGATCC GATCCCTGGA GCAGAGGATC 
GTGGAGCTGT CGGAAGCCAA CAAGCTTGCG GCAAACAGCA GTCTCTTCAC CCAGAGGAAC 
ATGAAGGCCC AGGAAGAGAT GATCTCAGAA CTCAGGCAGC AGAAATTTTA CCTGGAGACG 
CAGGCCGGAA AGCTGGAGGC CCAGAACCGA AAGCTGGAAG AGCAACTGGA GAAGATCAGC 
CACCAAGATC ACAGTGACAA GAGTCGGCTG CTGGAGCTGG AAACAAGGCT GAGGGAGGTC 
AGCCTGGAGC ACGAGGAGCA GAAGCTGGAG CTGAAGCGGC AGCTCACGGA GCTGCAGCTG 
TCCCTGCAGG AGCGCGAGTC CCAGCTGACG GCCCTGCAGG CCGCCCGGGC AGCGCTGGAG 
AGCCAGCTCC GCCAGGCGAA GACAGAGCTG GAGGAGACAA CCGCGGAAGC GGAGGAGGAG 
ATCCAGGCGC TCACGGCACA TCGAGATGAG ATCCAGCGCA AATTCGATGC CCTTCGCAAC 
AGCTGCACGG TCATCACCGA CCTGGAGGAG CAGCTGAACC AGCTCACCGA GGACAACGCC 
GAGCTCAACA ACCAAAACTT CTACCTGTCC AAACAACTCG ATGAGGCTTC CGGGGCCAAT 
GACGAGATTG TGCAGCTGCG GAGTGAGGTG GACCATCTCC GCCGTGAGAT CACGGAGCGG 
GAGATGCAGC TCACCAGCCA GAAGCAAACG ATGGAGGCTC TGAAGACGAC ATGCACCATG 
CTGGAGGAGC AGGTCCTGGA CCTGGAGGCC CTGAACGACG AGCTGCTGGA GAAGGAGCGC 
CAGTGGGAGG CCTGGCGGAG CGTCCTCGGC GACGAGAAGT CCCAGTTCGA GTGTCGAGTT 
CGAGAGCTAC AGAGGATGCT GGACACCGAG AAGCAGAGCA GGGCTAGGGC CGATCAGCGG 
ATCACCGAGT CGCGCCAGGT GGTGGAGTTG GCGGTGAAGG AACACAAGGC CGAGATTCTT 
GCTCTGCAGC AGGCTCTCAA GGAGCAGAAG CTCAAAGCCG AGAGCCTGTC GGACAAGCTC 
AACGACCTGG AGAAGAAGCA CGCCATGCTG GAGATGAACG CCCGGAGCTT ACAGCAGAAA 
CTAGAGACAG AGCGGGAGCT CAAACAGAGG CTTCTGGAGG AGCAAGCCAA ATTGCAGCAG 
CAGATGGACC TGCAGAAGAA CCACATCTTC AGACTGACGC AAGGGCTGCA GGAGGCGCTG 
GACCGGGCGG ATCTGCTGAA GACAGAAAGG AGCGACCTGG AGTACCAGCT GGAAAACATT 
CAGGTTCTCT ACTCTCACGA GAAAGTGAAA ATGGAAGGCA CAATCTCTCA GCAAACCAAA 
CTCATTGATT TCCTGCAAGC CAAAATGGAC CAGCCTGCTA AAAAGAAAAA GGTTCCTCTG 
CAGTACAATG AGCTGAAGCT AGCCCTGGAA AAGGAGAAAG CCCGATGCGC GGAGCTGGAG 
GAGGCCCTTC AGAAGACCCG CATCGAACTC CGCTCTGCGC GGGAGGAAGC TGCCCACCGC 
AAAGCCACGG ACCACCCGCA CCCGTCTACG CCAGCCACTG CGAGGCAGCA GATCGCCATG 
TCTGCCATTG TGCGGTCGCC CGAGCACCAG CCCAGTGCCA TGAGCCTGCT TGCCCCACCA 
TCCAGCCGCA GAAAGGAGTC GTCAACTCCA GAGGAATTCA GCCGGCGTCT GAAAGAGCGC 
ATGCACCACA ACATCCCTCA CCGGTTTAAT GTGGGCCTGA ACATGAGAGC CACCAAGTGC 
GCCGTGTGTC TGGATACTGT GCACTTTGGA CGCCAGGCAT CCAAATGCCT CGAATGTCAA 
GTGATGTGTC ACCCCAAATG CTCCACCTGC TTGCCCGCTA CCTGTGGCCT GCCAGCTGAA 
TATGCCACAC ACTTCACTGA GGCCTTCTGC CGGGACAAAA TGAACTCCCC GGGGCTCCAG 
AGCAAGGAGC CTGGCAGCAG CTTGCACCTG GAAGGGTGGA TGAAAGTGCC CAGAAATAAC 
AAACGGGGAC AGCAAGGCTG GGACAGGAAG TACATTGTTC TGGAGGGGTC AAAAGTCCTC 
ATCTATGACA ATGAAGCCAG AGAAGCTGGA CAGAGGCCGG TGGAAGAATT TGAGCTGTGC 
CTTCCTGACG GGGACGTATC TATTCATGGT GCCGTTGGTG CTTCAGAACT TGCAAATACC 
GCCAAAGCAG ATGTCCCATA CATCCTGAAG ATGGAGTCTC ACCCACACAC CACCTGCTGG 
CCTGGGAGAA CCCTCTACTT GCTAGCACCC AGCTTCCCCG ACAAGCAGCG CTGGGTCACC 
GCCTTAGAAT CTGTCGTCGC AGGTGGGAGA GTTTCTAGGG AAAAGGCCGA AGCCGATGCT 
AAATTACTTG GAAACTCTCT GCTGAAACTG GAGGGCGATG ACCGGCTTGA CATGAACTGC 
ACCCTGCCCT TCAGTGACCA GGTAGTGCTG GTGGGCACGG AGGAAGGCCT CTACGCCCTG 
AATGTCTTGA AAAACTCCTT AACCCACATC CCAGGGATTG GCGCAGTCTT CCAAATTTAC 
ATCATCAAGG ACCTGGAGAA GCTGCTCATG ATAGCAGGGG AAGAGCGGGC TCTGTGCCTG 
GTGGACGTGA AGAAGGTGAA GCAGTCCCTG GCGCAGTCAC ACCTGCCTGC CCAGCCCGAC 
GTCTCCCCCA ACATATTCGA AGCCGTCAAA GGCTGCCACT TGTTCGCTGC TGGCAAGATC 
GAGAACAGCC TGTGCATCTG CGCCGCTATG CCAAGCAAAG TCGTCATCCT CCGCTACAAT 
GACAACCTCA GCAAGTACTG CATCCGCAAG GAGATCGAGA CCTCAGAGCC CTGCAGCTGT 
ATCCACTTCA CCAACTACAG CATCCTCATT GGGACCAACA AATTCTATGA GATCGACATG 
AAGCAGTACA CGCTTGACGA GTTCCTGGAC AAGAACGACC ATTCCTTGGC ACCTGCTGTG 
TTCGCCTCCT CGTCCAACAG CTTCCCTGTC TCCATTGTGC AGGCGAACAG CGCCGGGCAG 
CGAGAAGAAT ACCTGCTGTG CTTCCACGAA TTTGGGGTGT TCGTGGATTC TTACGGAAGA 
CGTAGCCGCA CAGATGATCT CAAGTGGAGT CGCTTACCTC TGGCCTTCGC CTACAGAGAA 
CCTTATCTGT TTGTGACTCA CTTCAACTCC CTGGAAGTCA TTGAGATCCA GGCCAGATCC 
TCACTGGGGA GCCCTGCCCG AGCATATCTG GAAATTCCAA ACCCTCGCTA CCTGGGCCCC 
GCGATTTCCT CCGGAGCGAT TTACCTGGCC TCCTCATACC AGGACAAGTT AAGGGTCATA 
TGCTGCAAAG GAAACCTCGT GAAGGAGTCA GGCACTGAGC AGCACCGGGT GCCCTCCACC 
TCCCGCAGCA GCCCCAACAA GCGAGGCCCA CCAACATACA ACGAGCACAT CACCAAACGC 
GTGGCCTCCA GCCCGGCGCC ACCGGAAGGC CCCAGCCACC CCCGAGAGCC AAGCACACCG 
CACCGCTACC GAGACAGAGA GGGTCGGACA GAGCTGCGCA GGGACAAGTC TCCAGGCCGC 
CCTCTGGAGC GGGAGAAGTC CCCAGGCCGA ATGCTCAGCA CTAGGAGAGA GCGGTCCCCA 
GGGAGACTGT TTGAAGACAG CAGCAGGGGC CGGCTGCCTG CAGGAGCAGT GAGGACCCCA 
CTGTCCCAGG TTAACAAGGT CTGGGACCAG TCTTCAGTAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006530208.1
CDS	157..6198
Translation	MLKFKYGVRNPPEASASEPIASRASRLNLFFQGKPPLMTQQQMSALSREGMLDALFALFEECSQPALMKMKHVSSFVQKYSDTIAELRELQPSARDFEVRSLVGCGHFAEVQVVREKATGDVYAMKIMKKKALLAQEQVSFFEEERNILSRSTSPWIPQLQYAFQDKNNLYLVMEYQPGGDLLSLLNRYEDQLDESMIQFYLAELILAVHSVHQMGYVHRDIKPENILIDRTGHIKLVDFGSAAKMNSNKVDAKLPIGTPDYMAPEVLTVMNEDRRGTYGLDCDWWSVGVVAYEMVYGKTPFTEGTSARTFNNIMNFQRFLKFPDDPKVSSELLDLLQSLLCVQKERLKFEGLCCHPFFARTDWNNIRNSPPPFVPTLKSDDDTSNFDEPEKNSWVSSSVCQLSPSGFSGEELPFVGFSYSKALGYLGRSESVVSSLDSPAKVSSMEKKLLIKSKELQDSQDKCHKMEQEMTRLHRRVSEVEAVLSQKEVELKASETQRSLLEQDLATYITECSSLKRSLEQARMEVSQEDDKALQLLHDIREQSRKLQEIKEQEYQAQVEEMRLMMNQLEEDLVSARRRSDLYESELRESRLAAEEFKRKANECQHKLMKAKDQGKPEVGEYSKLEKINAEQQLKIQELQEKLEKAVKASTEATELLQNIRQAKERAERELEKLHNREDSSEGIKKKLVEAEELEEKHREAQVSAQHLEVHLKQKEQHYEEKIKVLDNQIKKDLADKESLENMMQRHEEEAHEKGKILSEQKAMINAMDSKIRSLEQRIVELSEANKLAANSSLFTQRNMKAQEEMISELRQQKFYLETQAGKLEAQNRKLEEQLEKISHQDHSDKSRLLELETRLREVSLEHEEQKLELKRQLTELQLSLQERESQLTALQAARAALESQLRQAKTELEETTAEAEEEIQALTAHRDEIQRKFDALRNSCTVITDLEEQLNQLTEDNAELNNQNFYLSKQLDEASGANDEIVQLRSEVDHLRREITEREMQLTSQKQTMEALKTTCTMLEEQVLDLEALNDELLEKERQWEAWRSVLGDEKSQFECRVRELQRMLDTEKQSRARADQRITESRQVVELAVKEHKAEILALQQALKEQKLKAESLSDKLNDLEKKHAMLEMNARSLQQKLETERELKQRLLEEQAKLQQQMDLQKNHIFRLTQGLQEALDRADLLKTERSDLEYQLENIQVLYSHEKVKMEGTISQQTKLIDFLQAKMDQPAKKKKVPLQYNELKLALEKEKARCAELEEALQKTRIELRSAREEAAHRKATDHPHPSTPATARQQIAMSAIVRSPEHQPSAMSLLAPPSSRRKESSTPEEFSRRLKERMHHNIPHRFNVGLNMRATKCAVCLDTVHFGRQASKCLECQVMCHPKCSTCLPATCGLPAEYATHFTEAFCRDKMNSPGLQSKEPGSSLHLEGWMKVPRNNKRGQQGWDRKYIVLEGSKVLIYDNEAREAGQRPVEEFELCLPDGDVSIHGAVGASELANTAKADVPYILKMESHPHTTCWPGRTLYLLAPSFPDKQRWVTALESVVAGGRVSREKAEADAKLLGNSLLKLEGDDRLDMNCTLPFSDQVVLVGTEEGLYALNVLKNSLTHIPGIGAVFQIYIIKDLEKLLMIAGEERALCLVDVKKVKQSLAQSHLPAQPDVSPNIFEAVKGCHLFAAGKIENSLCICAAMPSKVVILRYNDNLSKYCIRKEIETSEPCSCIHFTNYSILIGTNKFYEIDMKQYTLDEFLDKNDHSLAPAVFASSSNSFPVSIVQANSAGQREEYLLCFHEFGVFVDSYGRRSRTDDLKWSRLPLAFAYREPYLFVTHFNSLEVIEIQARSSLGSPARAYLEIPNPRYLGPAISSGAIYLASSYQDKLRVICCKGNLVKESGTEQHRVPSTSRSSPNKRGPPTYNEHITKRVASSPAPPEGPSHPREPSTPHRYRDREGRTELRRDKSPGRPLEREKSPGRMLSTRRERSPGRLFEDSSRGRLPAGAVRTPLSQVNKVWDQSSV

Target ORF information:

RefSeq Version	XM_006530145.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus citron (Cit), transcript variant X16, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006530145.3

ORF Insert Sequence:

ATGTTGAAGT TCAAGTATGG TGTGCGGAAC CCGCCGGAGG CCAGTGCCTC CGAGCCCATT 
GCCAGTCGGG CCTCCAGGCT AAATCTCTTC TTCCAGGGGA AACCGCCCCT CATGACTCAA 
CAGCAGATGT CTGCTCTTTC CCGGGAAGGG ATGCTAGACG CCCTCTTCGC TCTCTTTGAA 
GAGTGCAGCC AACCCGCCCT GATGAAGATG AAGCACGTGA GCAGCTTTGT CCAGAAGTAT 
TCCGACACCA TAGCCGAGTT GCGGGAGCTG CAGCCGTCGG CGAGAGACTT CGAAGTTCGA 
AGCCTTGTGG GCTGTGGTCA CTTCGCTGAA GTGCAGGTGG TTAGAGAGAA GGCGACCGGG 
GACGTCTATG CCATGAAAAT CATGAAGAAG AAGGCTTTGC TGGCCCAGGA ACAGGTTTCA 
TTTTTCGAGG AGGAGAGGAA CATATTATCT CGGAGCACGA GTCCTTGGAT CCCCCAGTTA 
CAGTACGCCT TTCAGGACAA AAATAACCTT TACCTGGTCA TGGAATATCA GCCTGGAGGG 
GATTTGCTGT CGCTTCTGAA CAGATACGAG GACCAATTAG ATGAGAGCAT GATCCAGTTT 
TACCTTGCTG AGCTGATTTT GGCTGTCCAC AGCGTGCACC AGATGGGATA TGTGCATCGA 
GACATCAAGC CCGAGAACAT CCTCATCGAC CGGACGGGAC ACATCAAGCT GGTGGATTTT 
GGATCAGCCG CTAAGATGAA TTCAAATAAG GTGGATGCCA AACTCCCCAT TGGGACCCCG 
GATTACATGG CTCCGGAAGT GTTGACCGTG ATGAACGAGG ACCGAAGGGG CACATACGGC 
TTGGACTGTG ACTGGTGGTC TGTCGGAGTT GTTGCTTATG AGATGGTTTA TGGGAAGACC 
CCATTCACAG AGGGAACCTC CGCCCGGACC TTCAACAACA TCATGAACTT CCAGCGGTTT 
TTGAAGTTCC CAGATGACCC CAAAGTTAGC AGTGAGCTCC TTGATCTGCT TCAGAGTCTG 
CTGTGTGTCC AGAAAGAGAG ACTGAAGTTC GAGGGTCTCT GCTGCCACCC TTTCTTTGCC 
AGAACGGACT GGAACAACAT CCGTAACTCT CCTCCCCCCT TCGTCCCCAC CCTCAAGTCT 
GACGATGACA CCTCCAATTT TGATGAACCA GAGAAGAATT CGTGGGTTTC ATCCTCTGTG 
TGCCAGCTGA GCCCCTCGGG CTTCTCAGGC GAAGAGCTGC CGTTTGTGGG ATTTTCGTAC 
AGCAAGGCAC TGGGGTATCT TGGTAGATCT GAGTCTGTCG TGTCGAGTCT GGACTCCCCT 
GCCAAGGTTA GCTCCATGGA AAAGAAACTT CTCATCAAAA GCAAAGAGCT CCAAGACTCC 
CAGGACAAGT GTCACAAGAT GGAGCAGGAA ATGACCCGGT TACATCGCAG AGTGTCAGAG 
GTGGAGGCTG TGCTTAGTCA GAAGGAGGTG GAGCTGAAGG CCTCTGAGAC TCAGAGATCC 
CTCCTGGAGC AGGACCTTGC TACCTACATC ACAGAATGCA GTAGCTTAAA GCGAAGTTTG 
GAGCAAGCGC GGATGGAGGT GTCCCAGGAG GATGACAAAG CTCTGCAGCT TCTCCACGAC 
ATCCGAGAGC AGAGCCGGAA GCTCCAGGAG ATCAAGGAGC AGGAGTACCA GGCTCAGGTG 
GAGGAGATGA GGCTGATGAT GAATCAGCTG GAAGAAGACC TGGTGTCAGC CCGCAGACGC 
AGCGATCTCT ACGAGTCTGA GCTGAGGGAG TCTCGGCTTG CCGCCGAGGA ATTCAAGCGG 
AAGGCAAACG AATGTCAGCA CAAACTGATG AAGGCTAAGG ACCAAGGGAA GCCTGAAGTG 
GGAGAATATT CCAAACTGGA GAAGATCAAT GCTGAGCAGC AGCTGAAGAT CCAGGAGCTC 
CAGGAGAAGC TGGAGAAGGC TGTAAAAGCC AGCACAGAGG CCACCGAGCT CCTGCAGAAT 
ATCCGCCAGG CAAAGGAGCG AGCAGAGCGG GAGCTAGAGA AGCTACACAA CCGGGAAGAC 
TCCTCCGAGG GCATCAAAAA GAAGCTGGTG GAAGCCGAGG AGCTGGAGGA GAAACATCGG 
GAGGCTCAGG TCTCAGCTCA ACATCTAGAA GTACACTTGA AACAGAAAGA ACAGCACTAC 
GAGGAAAAGA TCAAGGTATT GGACAATCAG ATAAAGAAGG ACCTGGCCGA CAAGGAGAGC 
CTGGAGAACA TGATGCAGAG ACACGAGGAG GAGGCCCACG AGAAGGGCAA GATCCTCAGC 
GAGCAGAAGG CGATGATCAA CGCGATGGAT TCCAAGATCC GATCCCTGGA GCAGAGGATC 
GTGGAGCTGT CGGAAGCCAA CAAGCTTGCG GCAAACAGCA GTCTCTTCAC CCAGAGGAAC 
ATGAAGGCCC AGGAAGAGAT GATCTCAGAA CTCAGGCAGC AGAAATTTTA CCTGGAGACG 
CAGGCCGGAA AGCTGGAGGC CCAGAACCGA AAGCTGGAAG AGCAACTGGA GAAGATCAGC 
CACCAAGATC ACAGTGACAA GAGTCGGCTG CTGGAGCTGG AAACAAGGCT GAGGGAGGTC 
AGCCTGGAGC ACGAGGAGCA GAAGCTGGAG CTGAAGCGGC AGCTCACGGA GCTGCAGCTG 
TCCCTGCAGG AGCGCGAGTC CCAGCTGACG GCCCTGCAGG CCGCCCGGGC AGCGCTGGAG 
AGCCAGCTCC GCCAGGCGAA GACAGAGCTG GAGGAGACAA CCGCGGAAGC GGAGGAGGAG 
ATCCAGGCGC TCACGGCACA TCGAGATGAG ATCCAGCGCA AATTCGATGC CCTTCGCAAC 
AGCTGCACGG TCATCACCGA CCTGGAGGAG CAGCTGAACC AGCTCACCGA GGACAACGCC 
GAGCTCAACA ACCAAAACTT CTACCTGTCC AAACAACTCG ATGAGGCTTC CGGGGCCAAT 
GACGAGATTG TGCAGCTGCG GAGTGAGGTG GACCATCTCC GCCGTGAGAT CACGGAGCGG 
GAGATGCAGC TCACCAGCCA GAAGCAAACG ATGGAGGCTC TGAAGACGAC ATGCACCATG 
CTGGAGGAGC AGGTCCTGGA CCTGGAGGCC CTGAACGACG AGCTGCTGGA GAAGGAGCGC 
CAGTGGGAGG CCTGGCGGAG CGTCCTCGGC GACGAGAAGT CCCAGTTCGA GTGTCGAGTT 
CGAGAGCTAC AGAGGATGCT GGACACCGAG AAGCAGAGCA GGGCTAGGGC CGATCAGCGG 
ATCACCGAGT CGCGCCAGGT GGTGGAGTTG GCGGTGAAGG AACACAAGGC CGAGATTCTT 
GCTCTGCAGC AGGCTCTCAA GGAGCAGAAG CTCAAAGCCG AGAGCCTGTC GGACAAGCTC 
AACGACCTGG AGAAGAAGCA CGCCATGCTG GAGATGAACG CCCGGAGCTT ACAGCAGAAA 
CTAGAGACAG AGCGGGAGCT CAAACAGAGG CTTCTGGAGG AGCAAGCCAA ATTGCAGCAG 
CAGATGGACC TGCAGAAGAA CCACATCTTC AGACTGACGC AAGGGCTGCA GGAGGCGCTG 
GACCGGGCGG ATCTGCTGAA GACAGAAAGG AGCGACCTGG AGTACCAGCT GGAAAACATT 
CAGGTTCTCT ACTCTCACGA GAAAGTGAAA ATGGAAGGCA CAATCTCTCA GCAAACCAAA 
CTCATTGATT TCCTGCAAGC CAAAATGGAC CAGCCTGCTA AAAAGAAAAA GGTTCCTCTG 
CAGTACAATG AGCTGAAGCT AGCCCTGGAA AAGGAGAAAG CCCGATGCGC GGAGCTGGAG 
GAGGCCCTTC AGAAGACCCG CATCGAACTC CGCTCTGCGC GGGAGGAAGC TGCCCACCGC 
AAAGCCACGG ACCACCCGCA CCCGTCTACG CCAGCCACTG CGAGGCAGCA GATCGCCATG 
TCTGCCATTG TGCGGTCGCC CGAGCACCAG CCCAGTGCCA TGAGCCTGCT TGCCCCACCA 
TCCAGCCGCA GAAAGGAGTC GTCAACTCCA GAGGAATTCA GCCGGCGTCT GAAAGAGCGC 
ATGCACCACA ACATCCCTCA CCGGTTTAAT GTGGGCCTGA ACATGAGAGC CACCAAGTGC 
GCCGTGTGTC TGGATACTGT GCACTTTGGA CGCCAGGCAT CCAAATGCCT CGAATGTCAA 
GTGATGTGTC ACCCCAAATG CTCCACCTGC TTGCCCGCTA CCTGTGGCCT GCCAGCTGAA 
TATGCCACAC ACTTCACTGA GGCCTTCTGC CGGGACAAAA TGAACTCCCC GGGGCTCCAG 
AGCAAGGAGC CTGGCAGCAG CTTGCACCTG GAAGGGTGGA TGAAAGTGCC CAGAAATAAC 
AAACGGGGAC AGCAAGGCTG GGACAGGAAG TACATTGTTC TGGAGGGGTC AAAAGTCCTC 
ATCTATGACA ATGAAGCCAG AGAAGCTGGA CAGAGGCCGG TGGAAGAATT TGAGCTGTGC 
CTTCCTGACG GGGACGTATC TATTCATGGT GCCGTTGGTG CTTCAGAACT TGCAAATACC 
GCCAAAGCAG ATGTCCCATA CATCCTGAAG ATGGAGTCTC ACCCACACAC CACCTGCTGG 
CCTGGGAGAA CCCTCTACTT GCTAGCACCC AGCTTCCCCG ACAAGCAGCG CTGGGTCACC 
GCCTTAGAAT CTGTCGTCGC AGGTGGGAGA GTTTCTAGGG AAAAGGCCGA AGCCGATGCT 
AAATTACTTG GAAACTCTCT GCTGAAACTG GAGGGCGATG ACCGGCTTGA CATGAACTGC 
ACCCTGCCCT TCAGTGACCA GGTAGTGCTG GTGGGCACGG AGGAAGGCCT CTACGCCCTG 
AATGTCTTGA AAAACTCCTT AACCCACATC CCAGGGATTG GCGCAGTCTT CCAAATTTAC 
ATCATCAAGG ACCTGGAGAA GCTGCTCATG ATAGCAGGGG AAGAGCGGGC TCTGTGCCTG 
GTGGACGTGA AGAAGGTGAA GCAGTCCCTG GCGCAGTCAC ACCTGCCTGC CCAGCCCGAC 
GTCTCCCCCA ACATATTCGA AGCCGTCAAA GGCTGCCACT TGTTCGCTGC TGGCAAGATC 
GAGAACAGCC TGTGCATCTG CGCCGCTATG CCAAGCAAAG TCGTCATCCT CCGCTACAAT 
GACAACCTCA GCAAGTACTG CATCCGCAAG GAGATCGAGA CCTCAGAGCC CTGCAGCTGT 
ATCCACTTCA CCAACTACAG CATCCTCATT GGGACCAACA AATTCTATGA GATCGACATG 
AAGCAGTACA CGCTTGACGA GTTCCTGGAC AAGAACGACC ATTCCTTGGC ACCTGCTGTG 
TTCGCCTCCT CGTCCAACAG CTTCCCTGTC TCCATTGTGC AGGCGAACAG CGCCGGGCAG 
CGAGAAGAAT ACCTGCTGTG CTTCCACGAA TTTGGGGTGT TCGTGGATTC TTACGGAAGA 
CGTAGCCGCA CAGATGATCT CAAGTGGAGT CGCTTACCTC TGGCCTTCGC CTACAGAGAA 
CCTTATCTGT TTGTGACTCA CTTCAACTCC CTGGAAGTCA TTGAGATCCA GGCCAGATCC 
TCACTGGGGA GCCCTGCCCG AGCATATCTG GAAATTCCAA ACCCTCGCTA CCTGGGCCCC 
GCGATTTCCT CCGGAGCGAT TTACCTGGCC TCCTCATACC AGGACAAGTT AAGGGTCATA 
TGCTGCAAAG GAAACCTCGT GAAGGAGTCA GGCACTGAGC AGCACCGGGT GCCCTCCACC 
TCCCGCAGCA GCCCCAACAA GCGAGGCCCA CCAACATACA ACGAGCACAT CACCAAACGC 
GTGGCCTCCA GCCCGGCGCC ACCGGAAGGC CCCAGCCACC CCCGAGAGCC AAGCACACCG 
CACCGCTACC GAGACAGAGA GGGTCGGACA GAGCTGCGCA GGGACAAGTC TCCAGGCCGC 
CCTCTGGAGC GGGAGAAGTC CCCAGGCCGA ATGCTCAGCA CTAGGAGAGA GCGGTCCCCA 
GGGAGACTGT TTGAAGACAG CAGCAGGGGC CGGCTGCCTG CAGGAGCAGT GAGGACCCCA 
CTGTCCCAGG TTAACAAGGT CTGGGACCAG TCTTCAGTAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cit ( XM_011248163.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011246465.1 Mus musculus citron (Cit), transcript variant X17, mRNA.

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CloneID	OMu67373
Clone ID Related Accession (Same CDS sequence)	XM_011248163.2 , XM_011248163.3
Accession Version	XM_011248163.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6039bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	citron Rho-interacting kinase isoform X14
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000071.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_011248163.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTGAAGT TCAAGTATGG TGTGCGGAAC CCGCCGGAGG CCAGTGCCTC CGAGCCCATT 
GCCAGTCGGG CCTCCAGGCT AAATCTCTTC TTCCAGGGGA AACCGCCCCT CATGACTCAA 
CAGCAGATGT CTGCTCTTTC CCGGGAAGGG ATGCTAGACG CCCTCTTCGC TCTCTTTGAA 
GAGTGCAGCC AACCCGCCCT GATGAAGATG AAGCACGTGA GCAGCTTTGT CCAGAAGTAT 
TCCGACACCA TAGCCGAGTT GCGGGAGCTG CAGCCGTCGG CGAGAGACTT CGAAGTTCGA 
AGCCTTGTGG GCTGTGGTCA CTTCGCTGAA GTGCAGGTGG TTAGAGAGAA GGCGACCGGG 
GACGTCTATG CCATGAAAAT CATGAAGAAG AAGGCTTTGC TGGCCCAGGA ACAGGTTTCA 
TTTTTCGAGG AGGAGAGGAA CATATTATCT CGGAGCACGA GTCCTTGGAT CCCCCAGTTA 
CAGTACGCCT TTCAGGACAA AAATAACCTT TACCTGGTCA TGGAATATCA GCCTGGAGGG 
GATTTGCTGT CGCTTCTGAA CAGATACGAG GACCAATTAG ATGAGAGCAT GATCCAGTTT 
TACCTTGCTG AGCTGATTTT GGCTGTCCAC AGCGTGCACC AGATGGGATA TGTGCATCGA 
GACATCAAGC CCGAGAACAT CCTCATCGAC CGGACGGGAC ACATCAAGCT GGTGGATTTT 
GGATCAGCCG CTAAGATGAA TTCAAATAAG GTGGATGCCA AACTCCCCAT TGGGACCCCG 
GATTACATGG CTCCGGAAGT GTTGACCGTG ATGAACGAGG ACCGAAGGGG CACATACGGC 
TTGGACTGTG ACTGGTGGTC TGTCGGAGTT GTTGCTTATG AGATGGTTTA TGGGAAGACC 
CCATTCACAG AGGGAACCTC CGCCCGGACC TTCAACAACA TCATGAACTT CCAGCGGTTT 
TTGAAGTTCC CAGATGACCC CAAAGTTAGC AGTGAGCTCC TTGATCTGCT TCAGAGTCTG 
CTGTGTGTCC AGAAAGAGAG ACTGAAGTTC GAGGGTCTCT GCTGCCACCC TTTCTTTGCC 
AGAACGGACT GGAACAACAT CCGTAACTCT CCTCCCCCCT TCGTCCCCAC CCTCAAGTCT 
GACGATGACA CCTCCAATTT TGATGAACCA GAGAAGAATT CGTGGGTTTC ATCCTCTGTG 
TGCCAGCTGA GCCCCTCGGG CTTCTCAGGC GAAGAGCTGC CGTTTGTGGG ATTTTCGTAC 
AGCAAGGCAC TGGGGTATCT TGGTAGATCT GAGTCTGTCG TGTCGAGTCT GGACTCCCCT 
GCCAAGGTTA GCTCCATGGA AAAGAAACTT CTCATCAAAA GCAAAGAGCT CCAAGACTCC 
CAGGACAAGT GTCACAAGAT GGAGCAGGAA ATGACCCGGT TACATCGCAG AGTGTCAGAG 
GTGGAGGCTG TGCTTAGTCA GAAGGAGGTG GAGCTGAAGG CCTCTGAGAC TCAGAGATCC 
CTCCTGGAGC AGGACCTTGC TACCTACATC ACAGAATGCA GTAGCTTAAA GCGAAGTTTG 
GAGCAAGCGC GGATGGAGGT GTCCCAGGAG GATGACAAAG CTCTGCAGCT TCTCCACGAC 
ATCCGAGAGC AGAGCCGGAA GCTCCAGGAG ATCAAGGAGC AGGAGTACCA GGCTCAGGTG 
GAGGAGATGA GGCTGATGAT GAATCAGCTG GAAGAAGACC TGGTGTCAGC CCGCAGACGC 
AGCGATCTCT ACGAGTCTGA GCTGAGGGAG TCTCGGCTTG CCGCCGAGGA ATTCAAGCGG 
AAGGCAAACG AATGTCAGCA CAAACTGATG AAGGCTAAGG ACCAAGGGAA GCCTGAAGTG 
GGAGAATATT CCAAACTGGA GAAGATCAAT GCTGAGCAGC AGCTGAAGAT CCAGGAGCTC 
CAGGAGAAGC TGGAGAAGGC TGTAAAAGCC AGCACAGAGG CCACCGAGCT CCTGCAGAAT 
ATCCGCCAGG CAAAGGAGCG AGCAGAGCGG GAGCTAGAGA AGCTACACAA CCGGGAAGAC 
TCCTCCGAGG GCATCAAAAA GAAGCTGGTG GAAGCCGAGG AGCTGGAGGA GAAACATCGG 
GAGGCTCAGG TCTCAGCTCA ACATCTAGAA GTACACTTGA AACAGAAAGA ACAGCACTAC 
GAGGAAAAGA TCAAGGTATT GGACAATCAG ATAAAGAAGG ACCTGGCCGA CAAGGAGAGC 
CTGGAGAACA TGATGCAGAG ACACGAGGAG GAGGCCCACG AGAAGGGCAA GATCCTCAGC 
GAGCAGAAGG CGATGATCAA CGCGATGGAT TCCAAGATCC GATCCCTGGA GCAGAGGATC 
GTGGAGCTGT CGGAAGCCAA CAAGCTTGCG GCAAACAGCA GTCTCTTCAC CCAGAGGAAC 
ATGAAGGCCC AGGAAGAGAT GATCTCAGAA CTCAGGCAGC AGAAATTTTA CCTGGAGACG 
CAGGCCGGAA AGCTGGAGGC CCAGAACCGA AAGCTGGAAG AGCAACTGGA GAAGATCAGC 
CACCAAGATC ACAGTGACAA GAGTCGGCTG CTGGAGCTGG AAACAAGGCT GAGGGAGGTC 
AGCCTGGAGC ACGAGGAGCA GAAGCTGGAG CTGAAGCGGC AGCTCACGGA GCTGCAGCTG 
TCCCTGCAGG AGCGCGAGTC CCAGCTGACG GCCCTGCAGG CCGCCCGGGC AGCGCTGGAG 
AGCCAGCTCC GCCAGGCGAA GACAGAGCTG GAGGAGACAA CCGCGGAAGC GGAGGAGGAG 
ATCCAGGCGC TCACGGCACA TCGAGATGAG ATCCAGCGCA AATTCGATGC CCTTCGCAAC 
AGCTGCACGG TCATCACCGA CCTGGAGGAG CAGCTGAACC AGCTCACCGA GGACAACGCC 
GAGCTCAACA ACCAAAACTT CTACCTGTCC AAACAACTCG ATGAGGCTTC CGGGGCCAAT 
GACGAGATTG TGCAGCTGCG GAGTGAGGTG GACCATCTCC GCCGTGAGAT CACGGAGCGG 
GAGATGCAGC TCACCAGCCA GAAGCAAACG ATGGAGGCTC TGAAGACGAC ATGCACCATG 
CTGGAGGAGC AGGTCCTGGA CCTGGAGGCC CTGAACGACG AGCTGCTGGA GAAGGAGCGC 
CAGTGGGAGG CCTGGCGGAG CGTCCTCGGC GACGAGAAGT CCCAGTTCGA GTGTCGAGTT 
CGAGAGCTAC AGAGGATGCT GGACACCGAG AAGCAGAGCA GGGCTAGGGC CGATCAGCGG 
ATCACCGAGT CGCGCCAGGT GGTGGAGTTG GCGGTGAAGG AACACAAGGC CGAGATTCTT 
GCTCTGCAGC AGGCTCTCAA GGAGCAGAAG CTCAAAGCCG AGAGCCTGTC GGACAAGCTC 
AACGACCTGG AGAAGAAGCA CGCCATGCTG GAGATGAACG CCCGGAGCTT ACAGCAGAAA 
CTAGAGACAG AGCGGGAGCT CAAACAGAGG CTTCTGGAGG AGCAAGCCAA ATTGCAGCAG 
CAGATGGACC TGCAGAAGAA CCACATCTTC AGACTGACGC AAGGGCTGCA GGAGGCGCTG 
GACCGGGCGG ATCTGCTGAA GACAGAAAGG AGCGACCTGG AGTACCAGCT GGAAAACATT 
CAGGTTCTCT ACTCTCACGA GAAAGTGAAA ATGGAAGGCA CAATCTCTCA GCAAACCAAA 
CTCATTGATT TCCTGCAAGC CAAAATGGAC CAGCCTGCTA AAAAGAAAAA GGTTCCTCTG 
CAGTACAATG AGCTGAAGCT AGCCCTGGAA AAGGAGAAAG CCCGATGCGC GGAGCTGGAG 
GAGGCCCTTC AGAAGACCCG CATCGAACTC CGCTCTGCGC GGGAGGAAGC TGCCCACCGC 
AAAGCCACGG ACCACCCGCA CCCGTCTACG CCAGCCACTG CGAGGCAGCA GATCGCCATG 
TCTGCCATTG TGCGGTCGCC CGAGCACCAG CCCAGTGCCA TGAGCCTGCT TGCCCCACCA 
TCCAGCCGCA GAAAGGAGTC GTCAACTCCA GAGGAATTCA GCCGGCGTCT GAAAGAGCGC 
ATGCACCACA ACATCCCTCA CCGGTTTAAT GTGGGCCTGA ACATGAGAGC CACCAAGTGC 
GCCGTGTGTC TGGATACTGT GCACTTTGGA CGCCAGGCAT CCAAATGCCT CGAATGTCAA 
GTGATGTGTC ACCCCAAATG CTCCACCTGC TTGCCCGCTA CCTGTGGCCT GCCAGCTGAA 
TATGCCACAC ACTTCACTGA GGCCTTCTGC CGGGACAAAA TGAACTCCCC GGGGCTCCAG 
AGCAAGGAGC CTGGCAGCAG CTTGCACCTG GAAGGGTGGA TGAAAGTGCC CAGAAATAAC 
AAACGGGGAC AGCAAGGCTG GGACAGGAAG TACATTGTTC TGGAGGGGTC AAAAGTCCTC 
ATCTATGACA ATGAAGCCAG AGAAGCTGGA CAGAGGCCGG TGGAAGAATT TGAGCTGTGC 
CTTCCTGACG GGGACGTATC TATTCATGGT GCCGTTGGTG CTTCAGAACT TGCAAATACC 
GCCAAAGCAG ATGTCCCATA CATCCTGAAG ATGGAGTCTC ACCCACACAC CACCTGCTGG 
CCTGGGAGAA CCCTCTACTT GCTAGCACCC AGCTTCCCCG ACAAGCAGCG CTGGGTCACC 
GCCTTAGAAT CTGTCGTCGC AGGTGGGAGA GTTTCTAGGG AAAAGGCCGA AGCCGATGCT 
AAATTACTTG GAAACTCTCT GCTGAAACTG GAGGGCGATG ACCGGCTTGA CATGAACTGC 
ACCCTGCCCT TCAGTGACCA GGTAGTGCTG GTGGGCACGG AGGAAGGCCT CTACGCCCTG 
AATGTCTTGA AAAACTCCTT AACCCACATC CCAGGGATTG GCGCAGTCTT CCAAATTTAC 
ATCATCAAGG ACCTGGAGAA GCTGCTCATG ATAGCAGGGG AAGAGCGGGC TCTGTGCCTG 
GTGGACGTGA AGAAGGTGAA GCAGTCCCTG GCGCAGTCAC ACCTGCCTGC CCAGCCCGAC 
GTCTCCCCCA ACATATTCGA AGCCGTCAAA GGCTGCCACT TGTTCGCTGC TGGCAAGATC 
GAGAACAGCC TGTGCATCTG CGCCGCTATG CCAAGCAAAG TCGTCATCCT CCGCTACAAT 
GACAACCTCA GCAAGTACTG CATCCGCAAG GAGATCGAGA CCTCAGAGCC CTGCAGCTGT 
ATCCACTTCA CCAACTACAG CATCCTCATT GGGACCAACA AATTCTATGA GATCGACATG 
AAGCAGTACA CGCTTGACGA GTTCCTGGAC AAGAACGACC ATTCCTTGGC ACCTGCTGTG 
TTCGCCTCCT CGTCCAACAG CTTCCCTGTC TCCATTGTGC AGGCGAACAG CGCCGGGCAG 
CGAGAAGAAT ACCTGCTGTG CTTCCACGAA TTTGGGGTGT TCGTGGATTC TTACGGAAGA 
CGTAGCCGCA CAGATGATCT CAAGTGGAGT CGCTTACCTC TGGCCTTCGC CTACAGAGAA 
CCTTATCTGT TTGTGACTCA CTTCAACTCC CTGGAAGTCA TTGAGATCCA GGCCAGATCC 
TCACTGGGGA GCCCTGCCCG AGCATATCTG GAAATTCCAA ACCCTCGCTA CCTGGGCCCC 
GCGATTTCCT CCGGAGCGAT TTACCTGGCC TCCTCATACC AGGACAAGTT AAGGGTCATA 
TGCTGCAAAG GAAACCTCGT GAAGGAGTCA GGCACTGAGC AGCACCGGGT GCCCTCCACC 
TCCCGCAGCC CCAACAAGCG AGGCCCACCA ACATACAACG AGCACATCAC CAAACGCGTG 
GCCTCCAGCC CGGCGCCACC GGAAGGCCCC AGCCACCCCC GAGAGCCAAG CACACCGCAC 
CGCTACCGAG ACAGAGAGGG TCGGACAGAG CTGCGCAGGG ACAAGTCTCC AGGCCGCCCT 
CTGGAGCGGG AGAAGTCCCC AGGCCGAATG CTCAGCACTA GGAGAGAGCG GTCCCCAGGG 
AGACTGTTTG AAGACAGCAG CAGGGGCCGG CTGCCTGCAG GAGCAGTGAG GACCCCACTG 
TCCCAGGTTA ACAAGGTCTG GGACCAGTCT TCAGTATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011246465.1
CDS	157..6195
Translation	MLKFKYGVRNPPEASASEPIASRASRLNLFFQGKPPLMTQQQMSALSREGMLDALFALFEECSQPALMKMKHVSSFVQKYSDTIAELRELQPSARDFEVRSLVGCGHFAEVQVVREKATGDVYAMKIMKKKALLAQEQVSFFEEERNILSRSTSPWIPQLQYAFQDKNNLYLVMEYQPGGDLLSLLNRYEDQLDESMIQFYLAELILAVHSVHQMGYVHRDIKPENILIDRTGHIKLVDFGSAAKMNSNKVDAKLPIGTPDYMAPEVLTVMNEDRRGTYGLDCDWWSVGVVAYEMVYGKTPFTEGTSARTFNNIMNFQRFLKFPDDPKVSSELLDLLQSLLCVQKERLKFEGLCCHPFFARTDWNNIRNSPPPFVPTLKSDDDTSNFDEPEKNSWVSSSVCQLSPSGFSGEELPFVGFSYSKALGYLGRSESVVSSLDSPAKVSSMEKKLLIKSKELQDSQDKCHKMEQEMTRLHRRVSEVEAVLSQKEVELKASETQRSLLEQDLATYITECSSLKRSLEQARMEVSQEDDKALQLLHDIREQSRKLQEIKEQEYQAQVEEMRLMMNQLEEDLVSARRRSDLYESELRESRLAAEEFKRKANECQHKLMKAKDQGKPEVGEYSKLEKINAEQQLKIQELQEKLEKAVKASTEATELLQNIRQAKERAERELEKLHNREDSSEGIKKKLVEAEELEEKHREAQVSAQHLEVHLKQKEQHYEEKIKVLDNQIKKDLADKESLENMMQRHEEEAHEKGKILSEQKAMINAMDSKIRSLEQRIVELSEANKLAANSSLFTQRNMKAQEEMISELRQQKFYLETQAGKLEAQNRKLEEQLEKISHQDHSDKSRLLELETRLREVSLEHEEQKLELKRQLTELQLSLQERESQLTALQAARAALESQLRQAKTELEETTAEAEEEIQALTAHRDEIQRKFDALRNSCTVITDLEEQLNQLTEDNAELNNQNFYLSKQLDEASGANDEIVQLRSEVDHLRREITEREMQLTSQKQTMEALKTTCTMLEEQVLDLEALNDELLEKERQWEAWRSVLGDEKSQFECRVRELQRMLDTEKQSRARADQRITESRQVVELAVKEHKAEILALQQALKEQKLKAESLSDKLNDLEKKHAMLEMNARSLQQKLETERELKQRLLEEQAKLQQQMDLQKNHIFRLTQGLQEALDRADLLKTERSDLEYQLENIQVLYSHEKVKMEGTISQQTKLIDFLQAKMDQPAKKKKVPLQYNELKLALEKEKARCAELEEALQKTRIELRSAREEAAHRKATDHPHPSTPATARQQIAMSAIVRSPEHQPSAMSLLAPPSSRRKESSTPEEFSRRLKERMHHNIPHRFNVGLNMRATKCAVCLDTVHFGRQASKCLECQVMCHPKCSTCLPATCGLPAEYATHFTEAFCRDKMNSPGLQSKEPGSSLHLEGWMKVPRNNKRGQQGWDRKYIVLEGSKVLIYDNEAREAGQRPVEEFELCLPDGDVSIHGAVGASELANTAKADVPYILKMESHPHTTCWPGRTLYLLAPSFPDKQRWVTALESVVAGGRVSREKAEADAKLLGNSLLKLEGDDRLDMNCTLPFSDQVVLVGTEEGLYALNVLKNSLTHIPGIGAVFQIYIIKDLEKLLMIAGEERALCLVDVKKVKQSLAQSHLPAQPDVSPNIFEAVKGCHLFAAGKIENSLCICAAMPSKVVILRYNDNLSKYCIRKEIETSEPCSCIHFTNYSILIGTNKFYEIDMKQYTLDEFLDKNDHSLAPAVFASSSNSFPVSIVQANSAGQREEYLLCFHEFGVFVDSYGRRSRTDDLKWSRLPLAFAYREPYLFVTHFNSLEVIEIQARSSLGSPARAYLEIPNPRYLGPAISSGAIYLASSYQDKLRVICCKGNLVKESGTEQHRVPSTSRSPNKRGPPTYNEHITKRVASSPAPPEGPSHPREPSTPHRYRDREGRTELRRDKSPGRPLEREKSPGRMLSTRRERSPGRLFEDSSRGRLPAGAVRTPLSQVNKVWDQSSV

Target ORF information:

RefSeq Version	XM_011248163.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus citron (Cit), transcript variant X17, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011248163.2

ORF Insert Sequence:

ATGTTGAAGT TCAAGTATGG TGTGCGGAAC CCGCCGGAGG CCAGTGCCTC CGAGCCCATT 
GCCAGTCGGG CCTCCAGGCT AAATCTCTTC TTCCAGGGGA AACCGCCCCT CATGACTCAA 
CAGCAGATGT CTGCTCTTTC CCGGGAAGGG ATGCTAGACG CCCTCTTCGC TCTCTTTGAA 
GAGTGCAGCC AACCCGCCCT GATGAAGATG AAGCACGTGA GCAGCTTTGT CCAGAAGTAT 
TCCGACACCA TAGCCGAGTT GCGGGAGCTG CAGCCGTCGG CGAGAGACTT CGAAGTTCGA 
AGCCTTGTGG GCTGTGGTCA CTTCGCTGAA GTGCAGGTGG TTAGAGAGAA GGCGACCGGG 
GACGTCTATG CCATGAAAAT CATGAAGAAG AAGGCTTTGC TGGCCCAGGA ACAGGTTTCA 
TTTTTCGAGG AGGAGAGGAA CATATTATCT CGGAGCACGA GTCCTTGGAT CCCCCAGTTA 
CAGTACGCCT TTCAGGACAA AAATAACCTT TACCTGGTCA TGGAATATCA GCCTGGAGGG 
GATTTGCTGT CGCTTCTGAA CAGATACGAG GACCAATTAG ATGAGAGCAT GATCCAGTTT 
TACCTTGCTG AGCTGATTTT GGCTGTCCAC AGCGTGCACC AGATGGGATA TGTGCATCGA 
GACATCAAGC CCGAGAACAT CCTCATCGAC CGGACGGGAC ACATCAAGCT GGTGGATTTT 
GGATCAGCCG CTAAGATGAA TTCAAATAAG GTGGATGCCA AACTCCCCAT TGGGACCCCG 
GATTACATGG CTCCGGAAGT GTTGACCGTG ATGAACGAGG ACCGAAGGGG CACATACGGC 
TTGGACTGTG ACTGGTGGTC TGTCGGAGTT GTTGCTTATG AGATGGTTTA TGGGAAGACC 
CCATTCACAG AGGGAACCTC CGCCCGGACC TTCAACAACA TCATGAACTT CCAGCGGTTT 
TTGAAGTTCC CAGATGACCC CAAAGTTAGC AGTGAGCTCC TTGATCTGCT TCAGAGTCTG 
CTGTGTGTCC AGAAAGAGAG ACTGAAGTTC GAGGGTCTCT GCTGCCACCC TTTCTTTGCC 
AGAACGGACT GGAACAACAT CCGTAACTCT CCTCCCCCCT TCGTCCCCAC CCTCAAGTCT 
GACGATGACA CCTCCAATTT TGATGAACCA GAGAAGAATT CGTGGGTTTC ATCCTCTGTG 
TGCCAGCTGA GCCCCTCGGG CTTCTCAGGC GAAGAGCTGC CGTTTGTGGG ATTTTCGTAC 
AGCAAGGCAC TGGGGTATCT TGGTAGATCT GAGTCTGTCG TGTCGAGTCT GGACTCCCCT 
GCCAAGGTTA GCTCCATGGA AAAGAAACTT CTCATCAAAA GCAAAGAGCT CCAAGACTCC 
CAGGACAAGT GTCACAAGAT GGAGCAGGAA ATGACCCGGT TACATCGCAG AGTGTCAGAG 
GTGGAGGCTG TGCTTAGTCA GAAGGAGGTG GAGCTGAAGG CCTCTGAGAC TCAGAGATCC 
CTCCTGGAGC AGGACCTTGC TACCTACATC ACAGAATGCA GTAGCTTAAA GCGAAGTTTG 
GAGCAAGCGC GGATGGAGGT GTCCCAGGAG GATGACAAAG CTCTGCAGCT TCTCCACGAC 
ATCCGAGAGC AGAGCCGGAA GCTCCAGGAG ATCAAGGAGC AGGAGTACCA GGCTCAGGTG 
GAGGAGATGA GGCTGATGAT GAATCAGCTG GAAGAAGACC TGGTGTCAGC CCGCAGACGC 
AGCGATCTCT ACGAGTCTGA GCTGAGGGAG TCTCGGCTTG CCGCCGAGGA ATTCAAGCGG 
AAGGCAAACG AATGTCAGCA CAAACTGATG AAGGCTAAGG ACCAAGGGAA GCCTGAAGTG 
GGAGAATATT CCAAACTGGA GAAGATCAAT GCTGAGCAGC AGCTGAAGAT CCAGGAGCTC 
CAGGAGAAGC TGGAGAAGGC TGTAAAAGCC AGCACAGAGG CCACCGAGCT CCTGCAGAAT 
ATCCGCCAGG CAAAGGAGCG AGCAGAGCGG GAGCTAGAGA AGCTACACAA CCGGGAAGAC 
TCCTCCGAGG GCATCAAAAA GAAGCTGGTG GAAGCCGAGG AGCTGGAGGA GAAACATCGG 
GAGGCTCAGG TCTCAGCTCA ACATCTAGAA GTACACTTGA AACAGAAAGA ACAGCACTAC 
GAGGAAAAGA TCAAGGTATT GGACAATCAG ATAAAGAAGG ACCTGGCCGA CAAGGAGAGC 
CTGGAGAACA TGATGCAGAG ACACGAGGAG GAGGCCCACG AGAAGGGCAA GATCCTCAGC 
GAGCAGAAGG CGATGATCAA CGCGATGGAT TCCAAGATCC GATCCCTGGA GCAGAGGATC 
GTGGAGCTGT CGGAAGCCAA CAAGCTTGCG GCAAACAGCA GTCTCTTCAC CCAGAGGAAC 
ATGAAGGCCC AGGAAGAGAT GATCTCAGAA CTCAGGCAGC AGAAATTTTA CCTGGAGACG 
CAGGCCGGAA AGCTGGAGGC CCAGAACCGA AAGCTGGAAG AGCAACTGGA GAAGATCAGC 
CACCAAGATC ACAGTGACAA GAGTCGGCTG CTGGAGCTGG AAACAAGGCT GAGGGAGGTC 
AGCCTGGAGC ACGAGGAGCA GAAGCTGGAG CTGAAGCGGC AGCTCACGGA GCTGCAGCTG 
TCCCTGCAGG AGCGCGAGTC CCAGCTGACG GCCCTGCAGG CCGCCCGGGC AGCGCTGGAG 
AGCCAGCTCC GCCAGGCGAA GACAGAGCTG GAGGAGACAA CCGCGGAAGC GGAGGAGGAG 
ATCCAGGCGC TCACGGCACA TCGAGATGAG ATCCAGCGCA AATTCGATGC CCTTCGCAAC 
AGCTGCACGG TCATCACCGA CCTGGAGGAG CAGCTGAACC AGCTCACCGA GGACAACGCC 
GAGCTCAACA ACCAAAACTT CTACCTGTCC AAACAACTCG ATGAGGCTTC CGGGGCCAAT 
GACGAGATTG TGCAGCTGCG GAGTGAGGTG GACCATCTCC GCCGTGAGAT CACGGAGCGG 
GAGATGCAGC TCACCAGCCA GAAGCAAACG ATGGAGGCTC TGAAGACGAC ATGCACCATG 
CTGGAGGAGC AGGTCCTGGA CCTGGAGGCC CTGAACGACG AGCTGCTGGA GAAGGAGCGC 
CAGTGGGAGG CCTGGCGGAG CGTCCTCGGC GACGAGAAGT CCCAGTTCGA GTGTCGAGTT 
CGAGAGCTAC AGAGGATGCT GGACACCGAG AAGCAGAGCA GGGCTAGGGC CGATCAGCGG 
ATCACCGAGT CGCGCCAGGT GGTGGAGTTG GCGGTGAAGG AACACAAGGC CGAGATTCTT 
GCTCTGCAGC AGGCTCTCAA GGAGCAGAAG CTCAAAGCCG AGAGCCTGTC GGACAAGCTC 
AACGACCTGG AGAAGAAGCA CGCCATGCTG GAGATGAACG CCCGGAGCTT ACAGCAGAAA 
CTAGAGACAG AGCGGGAGCT CAAACAGAGG CTTCTGGAGG AGCAAGCCAA ATTGCAGCAG 
CAGATGGACC TGCAGAAGAA CCACATCTTC AGACTGACGC AAGGGCTGCA GGAGGCGCTG 
GACCGGGCGG ATCTGCTGAA GACAGAAAGG AGCGACCTGG AGTACCAGCT GGAAAACATT 
CAGGTTCTCT ACTCTCACGA GAAAGTGAAA ATGGAAGGCA CAATCTCTCA GCAAACCAAA 
CTCATTGATT TCCTGCAAGC CAAAATGGAC CAGCCTGCTA AAAAGAAAAA GGTTCCTCTG 
CAGTACAATG AGCTGAAGCT AGCCCTGGAA AAGGAGAAAG CCCGATGCGC GGAGCTGGAG 
GAGGCCCTTC AGAAGACCCG CATCGAACTC CGCTCTGCGC GGGAGGAAGC TGCCCACCGC 
AAAGCCACGG ACCACCCGCA CCCGTCTACG CCAGCCACTG CGAGGCAGCA GATCGCCATG 
TCTGCCATTG TGCGGTCGCC CGAGCACCAG CCCAGTGCCA TGAGCCTGCT TGCCCCACCA 
TCCAGCCGCA GAAAGGAGTC GTCAACTCCA GAGGAATTCA GCCGGCGTCT GAAAGAGCGC 
ATGCACCACA ACATCCCTCA CCGGTTTAAT GTGGGCCTGA ACATGAGAGC CACCAAGTGC 
GCCGTGTGTC TGGATACTGT GCACTTTGGA CGCCAGGCAT CCAAATGCCT CGAATGTCAA 
GTGATGTGTC ACCCCAAATG CTCCACCTGC TTGCCCGCTA CCTGTGGCCT GCCAGCTGAA 
TATGCCACAC ACTTCACTGA GGCCTTCTGC CGGGACAAAA TGAACTCCCC GGGGCTCCAG 
AGCAAGGAGC CTGGCAGCAG CTTGCACCTG GAAGGGTGGA TGAAAGTGCC CAGAAATAAC 
AAACGGGGAC AGCAAGGCTG GGACAGGAAG TACATTGTTC TGGAGGGGTC AAAAGTCCTC 
ATCTATGACA ATGAAGCCAG AGAAGCTGGA CAGAGGCCGG TGGAAGAATT TGAGCTGTGC 
CTTCCTGACG GGGACGTATC TATTCATGGT GCCGTTGGTG CTTCAGAACT TGCAAATACC 
GCCAAAGCAG ATGTCCCATA CATCCTGAAG ATGGAGTCTC ACCCACACAC CACCTGCTGG 
CCTGGGAGAA CCCTCTACTT GCTAGCACCC AGCTTCCCCG ACAAGCAGCG CTGGGTCACC 
GCCTTAGAAT CTGTCGTCGC AGGTGGGAGA GTTTCTAGGG AAAAGGCCGA AGCCGATGCT 
AAATTACTTG GAAACTCTCT GCTGAAACTG GAGGGCGATG ACCGGCTTGA CATGAACTGC 
ACCCTGCCCT TCAGTGACCA GGTAGTGCTG GTGGGCACGG AGGAAGGCCT CTACGCCCTG 
AATGTCTTGA AAAACTCCTT AACCCACATC CCAGGGATTG GCGCAGTCTT CCAAATTTAC 
ATCATCAAGG ACCTGGAGAA GCTGCTCATG ATAGCAGGGG AAGAGCGGGC TCTGTGCCTG 
GTGGACGTGA AGAAGGTGAA GCAGTCCCTG GCGCAGTCAC ACCTGCCTGC CCAGCCCGAC 
GTCTCCCCCA ACATATTCGA AGCCGTCAAA GGCTGCCACT TGTTCGCTGC TGGCAAGATC 
GAGAACAGCC TGTGCATCTG CGCCGCTATG CCAAGCAAAG TCGTCATCCT CCGCTACAAT 
GACAACCTCA GCAAGTACTG CATCCGCAAG GAGATCGAGA CCTCAGAGCC CTGCAGCTGT 
ATCCACTTCA CCAACTACAG CATCCTCATT GGGACCAACA AATTCTATGA GATCGACATG 
AAGCAGTACA CGCTTGACGA GTTCCTGGAC AAGAACGACC ATTCCTTGGC ACCTGCTGTG 
TTCGCCTCCT CGTCCAACAG CTTCCCTGTC TCCATTGTGC AGGCGAACAG CGCCGGGCAG 
CGAGAAGAAT ACCTGCTGTG CTTCCACGAA TTTGGGGTGT TCGTGGATTC TTACGGAAGA 
CGTAGCCGCA CAGATGATCT CAAGTGGAGT CGCTTACCTC TGGCCTTCGC CTACAGAGAA 
CCTTATCTGT TTGTGACTCA CTTCAACTCC CTGGAAGTCA TTGAGATCCA GGCCAGATCC 
TCACTGGGGA GCCCTGCCCG AGCATATCTG GAAATTCCAA ACCCTCGCTA CCTGGGCCCC 
GCGATTTCCT CCGGAGCGAT TTACCTGGCC TCCTCATACC AGGACAAGTT AAGGGTCATA 
TGCTGCAAAG GAAACCTCGT GAAGGAGTCA GGCACTGAGC AGCACCGGGT GCCCTCCACC 
TCCCGCAGCC CCAACAAGCG AGGCCCACCA ACATACAACG AGCACATCAC CAAACGCGTG 
GCCTCCAGCC CGGCGCCACC GGAAGGCCCC AGCCACCCCC GAGAGCCAAG CACACCGCAC 
CGCTACCGAG ACAGAGAGGG TCGGACAGAG CTGCGCAGGG ACAAGTCTCC AGGCCGCCCT 
CTGGAGCGGG AGAAGTCCCC AGGCCGAATG CTCAGCACTA GGAGAGAGCG GTCCCCAGGG 
AGACTGTTTG AAGACAGCAG CAGGGGCCGG CTGCCTGCAG GAGCAGTGAG GACCCCACTG 
TCCCAGGTTA ACAAGGTCTG GGACCAGTCT TCAGTATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Citron Kinase Deficiency Leads to Chromosomal Instability and TP53-Sensitive Microcephaly.
Cell reports18(7)1674-1686(2017 Feb)
Bianchi FT,Tocco C,Pallavicini G,Liu Y,Vern? F,Merigliano C,Bonaccorsi S,El-Assawy N,Priano L,Gai M,Berto GE,Chiotto AM,Sgr? F,Caramello A,Tasca L,Ala U,Neri F,Oliviero S,Mauro A,Geley S,Gatti M,Di Cunto F

ASPM and CITK regulate spindle orientation by affecting the dynamics of astral microtubules.
EMBO reports17(10)1396-1409(2016 10)
Gai M,Bianchi FT,Vagnoni C,Vern? F,Bonaccorsi S,Pasquero S,Berto GE,Sgr? F,Chiotto AM,Annaratone L,Sapino A,Bergo A,Landsberger N,Bond J,Huttner WB,Di Cunto F

Tissue-specific control of midbody microtubule stability by Citron kinase through modulation of TUBB3 phosphorylation.
Cell death and differentiation23(5)801-13(2016 May)
Sgr? F,Bianchi FT,Falcone M,Pallavicini G,Gai M,Chiotto AM,Berto GE,Turco E,Chang YJ,Huttner WB,Di Cunto F

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Cit cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence: