Cd33 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Cd33
Entrez Gene ID	12489
Full Name	CD33 antigen
Synonyms	Siglec-3,gp67
General protein information	Preferred Names CD33 antigen Names myeloid cell surface antigen CD33 sialic acid-binding Ig-like lectin 3
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 7 Map Location: 7 B3\|7 28.25 cM

mRNA and Protein(s)

mRNA	Protein	Name
XM_006540590.3	XP_006540653.1	myeloid cell surface antigen CD33 isoform X1
NM_001111058.1	NP_001104528.1	myeloid cell surface antigen CD33 isoform 1 precursor
NM_021293.3	NP_067268.1	myeloid cell surface antigen CD33 isoform 2 precursor

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Systematic screening for skin, hair, and nail abnormalities in a large-scale knockout mouse program.
Sundberg JP, Dadras SS, Silva KA, Kennedy VE, Garland G, Murray SA, Sundberg BA, Schofield PN, Pratt CH
PloS one12(7)e0180682(2017)

High-throughput discovery of novel developmental phenotypes.
Dickinson ME, Flenniken AM, Ji X, Teboul L, Wong MD, White JK, Meehan TF, Weninger WJ, Westerberg H, Adissu H, Baker CN, Bower L, Brown JM, Caddle LB, Chiani F, Clary D, Cleak J, Daly MJ, Denegre JM, Doe B, Dolan ME, Edie SM, Fuchs H, Gailus-Durner V, Galli A, Gambadoro A, Gallegos J, Guo S, Horner NR, Hsu CW, Johnson SJ, Kalaga S, Keith LC, Lanoue L, Lawson TN, Lek M, Mark M, Marschall S, Mason J, McElwee ML, Newbigging S, Nutter LM, Peterson KA, Ramirez-Solis R, Rowland DJ, Ryder E, Samocha KE, Seavitt JR, Selloum M, Szoke-Kovacs Z, Tamura M, Trainor AG, Tudose I, Wakana S, Warren J, Wendling O, West DB, Wong L, Yoshiki A, , , , , , , , , MacArthur DG, Tocchini-Valentini GP, Gao X, Flicek P, Bradley A, Skarnes WC, Justice MJ, Parkinson HE, Moore M, Wells S, Braun RE, Svenson KL, de Angelis MH, Herault Y, Mohun T, Mallon AM, Henkelman RM, Brown SD, Adams DJ, Lloyd KC, McKerlie C, Beaudet AL, Bu?an M, Murray SA
Nature537(7621)508-514(2016 09)

Lectin galactoside-binding soluble 3 binding protein (LGALS3BP) is a tumor-associated immunomodulatory ligand for CD33-related Siglecs.
L?ubli H, Alisson-Silva F, Stanczak MA, Siddiqui SS, Deng L, Verhagen A, Varki N, Varki A
The Journal of biological chemistry289(48)33481-91(2014 Nov)

The International Mouse Phenotyping Consortium Web Portal, a unified point of access for knockout mice and related phenotyping data.
Koscielny G, Yaikhom G, Iyer V, Meehan TF, Morgan H, Atienza-Herrero J, Blake A, Chen CK, Easty R, Di Fenza A, Fiegel T, Grifiths M, Horne A, Karp NA, Kurbatova N, Mason JC, Matthews P, Oakley DJ, Qazi A, Regnart J, Retha A, Santos LA, Sneddon DJ, Warren J, Westerberg H, Wilson RJ, Melvin DG, Smedley D, Brown SD, Flicek P, Skarnes WC, Mallon AM, Parkinson H
Nucleic acids research42(Database issue)D802-9(2014 Jan)

Microglial CD33-related Siglec-E inhibits neurotoxicity by preventing the phagocytosis-associated oxidative burst.
Claude J, Linnartz-Gerlach B, Kudin AP, Kunz WS, Neumann H
The Journal of neuroscience : the official journal of the Society for Neuroscience33(46)18270-6(2013 Nov)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Lectin galactoside-binding soluble 3 binding protein (LGALS3BP) is a tumor-associated immunomodulatory ligand for CD33-related Siglecs.
Title: Lectin galactoside-binding soluble 3 binding protein (LGALS3BP) is a tumor-associated immunomodulatory ligand for CD33-related Siglecs.

CD33-related Siglec-E recognizes the intact neuron and has neuroprotective function by preventing phagocytosis.
Title: Microglial CD33-related Siglec-E inhibits neurotoxicity by preventing the phagocytosis-associated oxidative burst.

SOCS3 inhibited the CD33-induced block on cytokine-induced proliferation. SOCS3 and its target protein CD33 are degraded concomitantly concomitantly.
Title: CD33 responses are blocked by SOCS3 through accelerated proteasomal-mediated turnover.

mCD33 is expressed on myeloid precursors in the bone marrow
Title: CD33/Siglec-3 binding specificity, expression pattern, and consequences of gene deletion in mice.

The following Cd33 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Cd33 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu38655	XM_006540590.3 Latest version!	Mus musculus CD33 antigen (Cd33), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
OMu22058	NM_021293.3 Latest version!	Mus musculus CD33 antigen (Cd33), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$223.30 $319.00
OMu22088	NM_001111058.1 Latest version!	Mus musculus CD33 antigen (Cd33), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$188.30 $269.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Cd33 ( XM_006540590.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006540653.1 Mus musculus CD33 antigen (Cd33), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu38655
Clone ID Related Accession (Same CDS sequence)	XM_006540590.3
Accession Version	XM_006540590.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1080bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	myeloid cell surface antigen CD33 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000073.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006540590.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGACAG TGGAATGGGA GGAAGCCACA AAGAAGAGAC GGGAGCCGGA GGAACCTAGG 
ACCATTCACT GTGCTATGCT GTGGCCACTG CCGCTGTTCT TGCTGTGTGC AGGCTCCCTG 
GCTCAGGATT TAGAATTCCA GCTGGTGGCG CCCGAGTCAG TGACAGTCGA GGAGGGCCTA 
TGTGTCCATG TGCCCTGCAG TGTTTTCTAC CCCTCCATTA AGCTCACTTT AGGACCTGTG 
ACCGGCTCCT GGCTCCGGAA AGGGGTCAGT CTCCATGAAG ACTCTCCAGT GGCCACAAGT 
GACCCCAGAC AACTAGTGCA GAAGGCAACA CAGGGCAGAT TCCAACTCCT TGGGGACCCA 
CAGAAACATG ACTGTTCCCT GTTCATCAGA GATGCACAGA AAAATGACAC AGGAATGTAC 
TTCTTCAGAG TGGTCAGAGA ACCTTTTGTG AGATATTCTT ACAAAAAAAG CCAGCTGTCA 
CTGCATGTGA CCTCTCTATC ACGGACTCCT GACATTATAA TCCCGGGGAC CCTGGAGGCT 
GGCTATCCTA GCAATCTCAC CTGCTCTGTG CCCTGGGCTT GTGAGCAGGG GACACCCCCT 
ACTTTCTCCT GGATGTCAAC TGCCCTCACC TCCTTGAGTT CCCGAACCAC AGACTCCTCC 
GTGCTGACGT TCACACCTCA GCCTCAGGAC CATGGTACCA AACTCACCTG CTTGGTGACC 
TTCTCTGGAG CAGGTGTCAC TGTGGAAAGG ACCATCCAGC TCAATGTTAC CCGGAAATCA 
GGCCAGATGA GAGAGCTGGT CCTGGTGGCT GTGGGGGAGG CAACGGTCAA GCTCCTGATT 
CTTGGGCTCT GTCTCGTGTT TCTCATTGTG ATGTTCTGCA GAAGGAAGAC AACAAAGCTG 
TCAGTGCACA TGGGCTGTGA AAATCCTATC AAGGCTCATC AGCAGGACTC CAAGGTGCAC 
AGCAATCCTG AGAATCCAAG ACCCTTACAG AAGGACTCAC CTCAGGAACA GAGCAGTGTC 
CACACTAAGA TTTCCTTGGA TTTCATGGGA GGGAAGCCTC AGGAGTACTC AGAGATCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006540653.1
CDS	455..1534
Translation	MVTVEWEEATKKRREPEEPRTIHCAMLWPLPLFLLCAGSLAQDLEFQLVAPESVTVEEGLCVHVPCSVFYPSIKLTLGPVTGSWLRKGVSLHEDSPVATSDPRQLVQKATQGRFQLLGDPQKHDCSLFIRDAQKNDTGMYFFRVVREPFVRYSYKKSQLSLHVTSLSRTPDIIIPGTLEAGYPSNLTCSVPWACEQGTPPTFSWMSTALTSLSSRTTDSSVLTFTPQPQDHGTKLTCLVTFSGAGVTVERTIQLNVTRKSGQMRELVLVAVGEATVKLLILGLCLVFLIVMFCRRKTTKLSVHMGCENPIKAHQQDSKVHSNPENPRPLQKDSPQEQSSVHTKISLDFMGGKPQEYSEI

Target ORF information:

RefSeq Version	XM_006540590.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus CD33 antigen (Cd33), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006540590.3

ORF Insert Sequence:

ATGGTGACAG TGGAATGGGA GGAAGCCACA AAGAAGAGAC GGGAGCCGGA GGAACCTAGG 
ACCATTCACT GTGCTATGCT GTGGCCACTG CCGCTGTTCT TGCTGTGTGC AGGCTCCCTG 
GCTCAGGATT TAGAATTCCA GCTGGTGGCG CCCGAGTCAG TGACAGTCGA GGAGGGCCTA 
TGTGTCCATG TGCCCTGCAG TGTTTTCTAC CCCTCCATTA AGCTCACTTT AGGACCTGTG 
ACCGGCTCCT GGCTCCGGAA AGGGGTCAGT CTCCATGAAG ACTCTCCAGT GGCCACAAGT 
GACCCCAGAC AACTAGTGCA GAAGGCAACA CAGGGCAGAT TCCAACTCCT TGGGGACCCA 
CAGAAACATG ACTGTTCCCT GTTCATCAGA GATGCACAGA AAAATGACAC AGGAATGTAC 
TTCTTCAGAG TGGTCAGAGA ACCTTTTGTG AGATATTCTT ACAAAAAAAG CCAGCTGTCA 
CTGCATGTGA CCTCTCTATC ACGGACTCCT GACATTATAA TCCCGGGGAC CCTGGAGGCT 
GGCTATCCTA GCAATCTCAC CTGCTCTGTG CCCTGGGCTT GTGAGCAGGG GACACCCCCT 
ACTTTCTCCT GGATGTCAAC TGCCCTCACC TCCTTGAGTT CCCGAACCAC AGACTCCTCC 
GTGCTGACGT TCACACCTCA GCCTCAGGAC CATGGTACCA AACTCACCTG CTTGGTGACC 
TTCTCTGGAG CAGGTGTCAC TGTGGAAAGG ACCATCCAGC TCAATGTTAC CCGGAAATCA 
GGCCAGATGA GAGAGCTGGT CCTGGTGGCT GTGGGGGAGG CAACGGTCAA GCTCCTGATT 
CTTGGGCTCT GTCTCGTGTT TCTCATTGTG ATGTTCTGCA GAAGGAAGAC AACAAAGCTG 
TCAGTGCACA TGGGCTGTGA AAATCCTATC AAGGCTCATC AGCAGGACTC CAAGGTGCAC 
AGCAATCCTG AGAATCCAAG ACCCTTACAG AAGGACTCAC CTCAGGAACA GAGCAGTGTC 
CACACTAAGA TTTCCTTGGA TTTCATGGGA GGGAAGCCTC AGGAGTACTC AGAGATCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cd33 ( NM_021293.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_067268.1 Mus musculus CD33 antigen (Cd33), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OMu22058
Clone ID Related Accession (Same CDS sequence)	NM_021293.3
Accession Version	NM_021293.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1005bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-21
Organism	Mus musculus(house mouse)
Product	myeloid cell surface antigen CD33 isoform 2 precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BC132379.1 and AK155745.1. On Dec 1, 2007 this sequence version replaced NM_021293.2. Transcript Variant: This variant (2) lacks an exon in the 3' coding region that results in a frameshift compared to variant 1. The resulting protein (isoform 2) is shorter and has a distinct C-terminus compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK155745.1, S71403.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164133, SAMN01164135 [ECO:0000348] ##Evidence-Data-END##

ATGCTGTGGC CACTGCCGCT GTTCTTGCTG TGTGCAGGCT CCCTGGCTCA GGATTTAGAA 
TTCCAGCTGG TGGCGCCCGA GTCAGTGACA GTCGAGGAGG GCCTATGTGT CCATGTGCCC 
TGCAGTGTTT TCTACCCCTC CATTAAGCTC ACTTTAGGAC CTGTGACCGG CTCCTGGCTC 
CGGAAAGGGG TCAGTCTCCA TGAAGACTCT CCAGTGGCCA CAAGTGACCC CAGACAACTA 
GTGCAGAAGG CAACACAGGG CAGATTCCAA CTCCTTGGGG ACCCACAGAA ACATGACTGT 
TCCCTGTTCA TCAGAGATGC ACAGAAAAAT GACACAGGAA TGTACTTCTT CAGAGTGGTC 
AGAGAACCTT TTGTGAGATA TTCTTACAAA AAAAGCCAGC TGTCACTGCA TGTGACCTCT 
CTATCACGGA CTCCTGACAT TATAATCCCG GGGACCCTGG AGGCTGGCTA TCCTAGCAAT 
CTCACCTGCT CTGTGCCCTG GGCTTGTGAG CAGGGGACAC CCCCTACTTT CTCCTGGATG 
TCAACTGCCC TCACCTCCTT GAGTTCCCGA ACCACAGACT CCTCCGTGCT GACGTTCACA 
CCTCAGCCTC AGGACCATGG TACCAAACTC ACCTGCTTGG TGACCTTCTC TGGAGCAGGT 
GTCACTGTGG AAAGGACCAT CCAGCTCAAT GTTACCCGGA AATCAGGCCA GATGAGAGAG 
CTGGTCCTGG TGGCTGTGGG GGAGGCAACG GTCAAGCTCC TGATTCTTGG GCTCTGTCTC 
GTGTTTCTCA TTGTGATGTT CTGCAGAAGG AAGACAACAA AGCTGTCAGT GCACATGGGC 
TGTGAAAATC CTATCAAGGC TCATCAGCAG GACTCCAAGG TGCACAGCAA TCCTGAGAAT 
CCAAGACCCT TACAGAAGGA CTCACCTCAG GAACAGAGCA GTGTCCACAC TAAGATTTCC 
TTGGATTTCA TGGGAGGGAA GCCTCAGGAG TACTCAGAGA TCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_067268.1
CDS	78..1082
Translation	MLWPLPLFLLCAGSLAQDLEFQLVAPESVTVEEGLCVHVPCSVFYPSIKLTLGPVTGSWLRKGVSLHEDSPVATSDPRQLVQKATQGRFQLLGDPQKHDCSLFIRDAQKNDTGMYFFRVVREPFVRYSYKKSQLSLHVTSLSRTPDIIIPGTLEAGYPSNLTCSVPWACEQGTPPTFSWMSTALTSLSSRTTDSSVLTFTPQPQDHGTKLTCLVTFSGAGVTVERTIQLNVTRKSGQMRELVLVAVGEATVKLLILGLCLVFLIVMFCRRKTTKLSVHMGCENPIKAHQQDSKVHSNPENPRPLQKDSPQEQSSVHTKISLDFMGGKPQEYSEI

Target ORF information:

RefSeq Version	NM_021293.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus CD33 antigen (Cd33), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_021293.3

ORF Insert Sequence:

ATGCTGTGGC CACTGCCGCT GTTCTTGCTG TGTGCAGGCT CCCTGGCTCA GGATTTAGAA 
TTCCAGCTGG TGGCGCCCGA GTCAGTGACA GTCGAGGAGG GCCTATGTGT CCATGTGCCC 
TGCAGTGTTT TCTACCCCTC CATTAAGCTC ACTTTAGGAC CTGTGACCGG CTCCTGGCTC 
CGGAAAGGGG TCAGTCTCCA TGAAGACTCT CCAGTGGCCA CAAGTGACCC CAGACAACTA 
GTGCAGAAGG CAACACAGGG CAGATTCCAA CTCCTTGGGG ACCCACAGAA ACATGACTGT 
TCCCTGTTCA TCAGAGATGC ACAGAAAAAT GACACAGGAA TGTACTTCTT CAGAGTGGTC 
AGAGAACCTT TTGTGAGATA TTCTTACAAA AAAAGCCAGC TGTCACTGCA TGTGACCTCT 
CTATCACGGA CTCCTGACAT TATAATCCCG GGGACCCTGG AGGCTGGCTA TCCTAGCAAT 
CTCACCTGCT CTGTGCCCTG GGCTTGTGAG CAGGGGACAC CCCCTACTTT CTCCTGGATG 
TCAACTGCCC TCACCTCCTT GAGTTCCCGA ACCACAGACT CCTCCGTGCT GACGTTCACA 
CCTCAGCCTC AGGACCATGG TACCAAACTC ACCTGCTTGG TGACCTTCTC TGGAGCAGGT 
GTCACTGTGG AAAGGACCAT CCAGCTCAAT GTTACCCGGA AATCAGGCCA GATGAGAGAG 
CTGGTCCTGG TGGCTGTGGG GGAGGCAACG GTCAAGCTCC TGATTCTTGG GCTCTGTCTC 
GTGTTTCTCA TTGTGATGTT CTGCAGAAGG AAGACAACAA AGCTGTCAGT GCACATGGGC 
TGTGAAAATC CTATCAAGGC TCATCAGCAG GACTCCAAGG TGCACAGCAA TCCTGAGAAT 
CCAAGACCCT TACAGAAGGA CTCACCTCAG GAACAGAGCA GTGTCCACAC TAAGATTTCC 
TTGGATTTCA TGGGAGGGAA GCCTCAGGAG TACTCAGAGA TCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cd33 ( NM_001111058.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001104528.1 Mus musculus CD33 antigen (Cd33), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OMu22088
Clone ID Related Accession (Same CDS sequence)	NM_001111058.1
Accession Version	NM_001111058.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1212bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-21
Organism	Mus musculus(house mouse)
Product	myeloid cell surface antigen CD33 isoform 1 precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC154108.3. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer protein (isoform 1). Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMN01164133, SAMN01164135 [ECO:0000348] ##Evidence-Data-END##

ATGCTGTGGC CACTGCCGCT GTTCTTGCTG TGTGCAGGCT CCCTGGCTCA GGATTTAGAA 
TTCCAGCTGG TGGCGCCCGA GTCAGTGACA GTCGAGGAGG GCCTATGTGT CCATGTGCCC 
TGCAGTGTTT TCTACCCCTC CATTAAGCTC ACTTTAGGAC CTGTGACCGG CTCCTGGCTC 
CGGAAAGGGG TCAGTCTCCA TGAAGACTCT CCAGTGGCCA CAAGTGACCC CAGACAACTA 
GTGCAGAAGG CAACACAGGG CAGATTCCAA CTCCTTGGGG ACCCACAGAA ACATGACTGT 
TCCCTGTTCA TCAGAGATGC ACAGAAAAAT GACACAGGAA TGTACTTCTT CAGAGTGGTC 
AGAGAACCTT TTGTGAGATA TTCTTACAAA AAAAGCCAGC TGTCACTGCA TGTGACCTCT 
CTATCACGGA CTCCTGACAT TATAATCCCG GGGACCCTGG AGGCTGGCTA TCCTAGCAAT 
CTCACCTGCT CTGTGCCCTG GGCTTGTGAG CAGGGGACAC CCCCTACTTT CTCCTGGATG 
TCAACTGCCC TCACCTCCTT GAGTTCCCGA ACCACAGACT CCTCCGTGCT GACGTTCACA 
CCTCAGCCTC AGGACCATGG TACCAAACTC ACCTGCTTGG TGACCTTCTC TGGAGCAGGT 
GTCACTGTGG AAAGGACCAT CCAGCTCAAT GTTACCCGGA AATCAGGCCA GATGAGAGAG 
CTGGTCCTGG TGGCTGTGGG GGAGGCAACG GTCAAGCTCC TGATTCTTGG GCTCTGTCTC 
GTGTTTCTCA TTGTGATGTT CTGCAGAAGG AAGACAACAA AGCTGTCAGT GCACATGGGC 
TGTGAAAATC CTATCAAGAG GCAGGAAGCG ATCACATCCT ATAATCACTG CCTATCTCCA 
ACTGCATCTG ATGCTGTGAC TCCAGGATGT TCCATACACA GGCTCATCAG CAGGACTCCA 
AGGTGCACAG CAATCCTGAG AATCCAAGAC CCTTACAGAA GGACTCACCT CAGGAACAGA 
GCAGTGTCCA CACTAAGATT TCCTTGGATT TCATGGGAGG GAAGCCTCAG GAGTACTCAG 
AGATCTAAAT GCACCAAGCT TTGCTCTCCT GTGAAGAACC TGTGTCCTCT ATGGCTTCCT 
GTGGATAATT CCTGCATCCC TCTGATCCCA GAGTGGGTTA TGCTGCTGTG TGTGAGTCTT 
ACACTCAGTT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001104528.1
CDS	78..1289
Translation	MLWPLPLFLLCAGSLAQDLEFQLVAPESVTVEEGLCVHVPCSVFYPSIKLTLGPVTGSWLRKGVSLHEDSPVATSDPRQLVQKATQGRFQLLGDPQKHDCSLFIRDAQKNDTGMYFFRVVREPFVRYSYKKSQLSLHVTSLSRTPDIIIPGTLEAGYPSNLTCSVPWACEQGTPPTFSWMSTALTSLSSRTTDSSVLTFTPQPQDHGTKLTCLVTFSGAGVTVERTIQLNVTRKSGQMRELVLVAVGEATVKLLILGLCLVFLIVMFCRRKTTKLSVHMGCENPIKRQEAITSYNHCLSPTASDAVTPGCSIHRLISRTPRCTAILRIQDPYRRTHLRNRAVSTLRFPWISWEGSLRSTQRSKCTKLCSPVKNLCPLWLPVDNSCIPLIPEWVMLLCVSLTLS

Target ORF information:

RefSeq Version	NM_001111058.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus CD33 antigen (Cd33), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001111058.1

ORF Insert Sequence:

ATGCTGTGGC CACTGCCGCT GTTCTTGCTG TGTGCAGGCT CCCTGGCTCA GGATTTAGAA 
TTCCAGCTGG TGGCGCCCGA GTCAGTGACA GTCGAGGAGG GCCTATGTGT CCATGTGCCC 
TGCAGTGTTT TCTACCCCTC CATTAAGCTC ACTTTAGGAC CTGTGACCGG CTCCTGGCTC 
CGGAAAGGGG TCAGTCTCCA TGAAGACTCT CCAGTGGCCA CAAGTGACCC CAGACAACTA 
GTGCAGAAGG CAACACAGGG CAGATTCCAA CTCCTTGGGG ACCCACAGAA ACATGACTGT 
TCCCTGTTCA TCAGAGATGC ACAGAAAAAT GACACAGGAA TGTACTTCTT CAGAGTGGTC 
AGAGAACCTT TTGTGAGATA TTCTTACAAA AAAAGCCAGC TGTCACTGCA TGTGACCTCT 
CTATCACGGA CTCCTGACAT TATAATCCCG GGGACCCTGG AGGCTGGCTA TCCTAGCAAT 
CTCACCTGCT CTGTGCCCTG GGCTTGTGAG CAGGGGACAC CCCCTACTTT CTCCTGGATG 
TCAACTGCCC TCACCTCCTT GAGTTCCCGA ACCACAGACT CCTCCGTGCT GACGTTCACA 
CCTCAGCCTC AGGACCATGG TACCAAACTC ACCTGCTTGG TGACCTTCTC TGGAGCAGGT 
GTCACTGTGG AAAGGACCAT CCAGCTCAAT GTTACCCGGA AATCAGGCCA GATGAGAGAG 
CTGGTCCTGG TGGCTGTGGG GGAGGCAACG GTCAAGCTCC TGATTCTTGG GCTCTGTCTC 
GTGTTTCTCA TTGTGATGTT CTGCAGAAGG AAGACAACAA AGCTGTCAGT GCACATGGGC 
TGTGAAAATC CTATCAAGAG GCAGGAAGCG ATCACATCCT ATAATCACTG CCTATCTCCA 
ACTGCATCTG ATGCTGTGAC TCCAGGATGT TCCATACACA GGCTCATCAG CAGGACTCCA 
AGGTGCACAG CAATCCTGAG AATCCAAGAC CCTTACAGAA GGACTCACCT CAGGAACAGA 
GCAGTGTCCA CACTAAGATT TCCTTGGATT TCATGGGAGG GAAGCCTCAG GAGTACTCAG 
AGATCTAAAT GCACCAAGCT TTGCTCTCCT GTGAAGAACC TGTGTCCTCT ATGGCTTCCT 
GTGGATAATT CCTGCATCCC TCTGATCCCA GAGTGGGTTA TGCTGCTGTG TGTGAGTCTT 
ACACTCAGTT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Systematic screening for skin, hair, and nail abnormalities in a large-scale knockout mouse program.
PloS one12(7)e0180682(2017)
Sundberg JP,Dadras SS,Silva KA,Kennedy VE,Garland G,Murray SA,Sundberg BA,Schofield PN,Pratt CH

High-throughput discovery of novel developmental phenotypes.
Nature537(7621)508-514(2016 09)
Dickinson ME,Flenniken AM,Ji X,Teboul L,Wong MD,White JK,Meehan TF,Weninger WJ,Westerberg H,Adissu H,Baker CN,Bower L,Brown JM,Caddle LB,Chiani F,Clary D,Cleak J,Daly MJ,Denegre JM,Doe B,Dolan ME,Edie SM,Fuchs H,Gailus-Durner V,Galli A,Gambadoro A,Gallegos J,Guo S,Horner NR,Hsu CW,Johnson SJ,Kalaga S,Keith LC,Lanoue L,Lawson TN,Lek M,Mark M,Marschall S,Mason J,McElwee ML,Newbigging S,Nutter LM,Peterson KA,Ramirez-Solis R,Rowland DJ,Ryder E,Samocha KE,Seavitt JR,Selloum M,Szoke-Kovacs Z,Tamura M,Trainor AG,Tudose I,Wakana S,Warren J,Wendling O,West DB,Wong L,Yoshiki A,,,,,,,,,MacArthur DG,Tocchini-Valentini GP,Gao X,Flicek P,Bradley A,Skarnes WC,Justice MJ,Parkinson HE,Moore M,Wells S,Braun RE,Svenson KL,de Angelis MH,Herault Y,Mohun T,Mallon AM,Henkelman RM,Brown SD,Adams DJ,Lloyd KC,McKerlie C,Beaudet AL,Bu?an M,Murray SA

Lectin galactoside-binding soluble 3 binding protein (LGALS3BP) is a tumor-associated immunomodulatory ligand for CD33-related Siglecs.
The Journal of biological chemistry289(48)33481-91(2014 Nov)
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The International Mouse Phenotyping Consortium Web Portal, a unified point of access for knockout mice and related phenotyping data.
Nucleic acids research42(Database issue)D802-9(2014 Jan)
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Microglial CD33-related Siglec-E inhibits neurotoxicity by preventing the phagocytosis-associated oxidative burst.
The Journal of neuroscience : the official journal of the Society for Neuroscience33(46)18270-6(2013 Nov)
Claude J,Linnartz-Gerlach B,Kudin AP,Kunz WS,Neumann H

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Cd33 cDNA ORF clone, Mus musculus(house mouse)

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