Car4 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Car4
Entrez Gene ID	12351
Full Name	carbonic anhydrase 4
Synonyms	AW456718,Ca4
General protein information	Preferred Names carbonic anhydrase 4 Names carbonic anhydrase 4 CA-IV carbonate dehydratase IV carbonic anhydrase IV
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 11 Map Location: 11\|11 C

mRNA and Protein(s)

mRNA	Protein	Name
XM_006532095.3	XP_006532158.1	carbonic anhydrase 4 isoform X1
XM_006532095.4	XP_006532158.1	carbonic anhydrase 4 isoform X1
NM_007607.2	NP_031633.1	carbonic anhydrase 4 precursor

Pathways from BioSystems

Homologies

Danio rerio (zebrafish)	ca4a	NP_001107879.1
Xenopus tropicalis (tropical clawed frog)	ca4	XP_002933882.2
Pan troglodytes (chimpanzee)	CA4	XP_001140977.1
Danio rerio (zebrafish)	ca4c	XP_003200928.1
Homo sapiens (human)	CA4	NP_000708.1
Bos taurus (cattle)	CA4	NP_776322.1
Gallus gallus (chicken)	CA4	XP_415893.1
Canis lupus familiaris (dog)	CA4	XP_537711.3
Mus musculus (house mouse)	Car4	NP_031633.1
Danio rerio (zebrafish)	ca4b	NP_001159683.1
	CA4	XP_001107970.2
Rattus norvegicus (Norway rat)	Car4	NP_062047.1

Gene Ontology
Bibliography

Related articles in PubMed

High-throughput discovery of novel developmental phenotypes.
Dickinson ME, Flenniken AM, Ji X, Teboul L, Wong MD, White JK, Meehan TF, Weninger WJ, Westerberg H, Adissu H, Baker CN, Bower L, Brown JM, Caddle LB, Chiani F, Clary D, Cleak J, Daly MJ, Denegre JM, Doe B, Dolan ME, Edie SM, Fuchs H, Gailus-Durner V, Galli A, Gambadoro A, Gallegos J, Guo S, Horner NR, Hsu CW, Johnson SJ, Kalaga S, Keith LC, Lanoue L, Lawson TN, Lek M, Mark M, Marschall S, Mason J, McElwee ML, Newbigging S, Nutter LM, Peterson KA, Ramirez-Solis R, Rowland DJ, Ryder E, Samocha KE, Seavitt JR, Selloum M, Szoke-Kovacs Z, Tamura M, Trainor AG, Tudose I, Wakana S, Warren J, Wendling O, West DB, Wong L, Yoshiki A, , , , , , , , , MacArthur DG, Tocchini-Valentini GP, Gao X, Flicek P, Bradley A, Skarnes WC, Justice MJ, Parkinson HE, Moore M, Wells S, Braun RE, Svenson KL, de Angelis MH, Herault Y, Mohun T, Mallon AM, Henkelman RM, Brown SD, Adams DJ, Lloyd KC, McKerlie C, Beaudet AL, Bu?an M, Murray SA
Nature537(7621)508-514(2016 09)

Bcl11b/Ctip2 is required for development of lingual papillae in mice.
Nishiguchi Y, Ohmoto M, Koki J, Enomoto T, Kominami R, Matsumoto I, Hirota J
Developmental biology416(1)98-110(2016 08)

Normal Fertility Requires the Expression of Carbonic Anhydrases II and IV in Sperm.
Wandernoth PM, Mannowetz N, Szczyrba J, Grannemann L, Wolf A, Becker HM, Sly WS, Wennemuth G
The Journal of biological chemistry290(49)29202-16(2015 Dec)

Thyroid hormone drives the expression of mouse carbonic anhydrase Car4 in kidney, lung and brain.
Vujovic M, Dudazy-Gralla S, H?rd J, Solsj? P, Warner A, Vennstr?m B, Mittag J
Molecular and cellular endocrinology41619-26(2015 Nov)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

two CA isoforms, CAII and CAIV, are distributed along the epididymal epithelium and appear with the onset of puberty
Title: Normal Fertility Requires the Expression of Carbonic Anhydrases II and IV in Sperm.

Mice with a mutant thyroid hormone receptor alpha1 have permanently reduced Car4 in lung and brain. These findings identify a previously unknown marker of thyroid hormone receptor alpha1 action in brain and lung.
Title: Thyroid hormone drives the expression of mouse carbonic anhydrase Car4 in kidney, lung and brain.

eosinophils express CAR4 following IL-5 or allergen exposure, and that CAR4 is involved in regulating the lung transcriptome associated with allergic airway inflammation
Title: Carbonic anhydrase IV is expressed on IL-5-activated murine eosinophils.

membrane-associated CA IV contributes robust catalytic activity intracellularly, and this activity participates in regulating H(+) dynamics in the cytosol in neurons
Title: GPI-anchored carbonic anhydrase IV displays both intra- and extracellular activity in cRNA-injected oocytes and in mouse neurons.

Data show a 32.13% reduction in total CA activity in CA IV(-/-) mice.
Title: Role of carbonic anhydrase IV in the bicarbonate-mediated activation of murine and human sperm.

The following Car4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Car4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu00468	NM_007607.2 Latest version!	Mus musculus carbonic anhydrase 4 (Car4), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$188.30 $269.00
OMu45490	XM_006532095.4 Latest version!	Mus musculus carbonic anhydrase 4 (Car4), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$139.30 $199.00
View Old Accession Versions >>
OMu45490	XM_006532095.3	Mus musculus carbonic anhydrase 4 (Car4), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$139.30 $199.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Car4 ( NM_007607.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_031633.1 Mus musculus carbonic anhydrase 4 (Car4), mRNA.

Price & Availability↑

CloneID	OMu00468
Clone ID Related Accession (Same CDS sequence)	NM_007607.2
Accession Version	NM_007607.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 918bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-27
Organism	Mus musculus(house mouse)
Product	carbonic anhydrase 4 precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AL669859.5, AK165399.1 and BM935015.1. On Jun 11, 2009 this sequence version replaced NM_007607.1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK154754.1, AK165399.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCAGCTCC TTCTTGCTCT GCTGGCGCTG GCATACGTGG CCCCCTCTAC TGAAGACTCA 
GGCTGGTGCT ATGAGATTCA AACCAAGGAT CCTAGAAGCA GTTGCTTGGG GCCTGAGAAG 
TGGCCTGGAG CCTGTAAGGA GAATCAGCAG TCCCCCATCA ACATTGTCAC TGCTAGGACA 
AAGGTGAACC CCAGACTGAC ACCCTTCATC CTCGTCGGCT ATGACCAAAA GCAACAGTGG 
CCAATTAAGA ACAATCAACA CACAGTGGAG ATGACGCTGG GGGGAGGTGC GTGCATTATC 
GGAGGAGACC TGCCCGCCCG GTACGAGGCT GTACAGTTGC ACCTGCACTG GTCAAATGGG 
AATGACAACG GTTCAGAGCA CAGTATTGAT GGGAGACACT TTGCCATGGA GATGCACATC 
GTGCACAAGA AGTTGACATC TAGCAAGGAG GACTCGAAGG ACAAGTTTGC AGTGCTGGCA 
TTTATGATTG AGGTAGGAGA CAAGGTGAAC AAGGGCTTCC AGCCCCTGGT GGAAGCACTG 
CCCAGTATCT CCAAACCCCA TTCAACCTCT ACAGTGAGGG AGAGCAGTTT GCAGGACATG 
CTTCCCCCGA GCACCAAAAT GTATACCTAC TTCCGTTACA ATGGCTCACT AACCACACCA 
AACTGTGATG AGACTGTCAT CTGGACTGTG TACAAACAAC CCATCAAGAT CCACAAAAAC 
CAGTTCCTGG AATTCTCAAA AAATCTGTAC TATGACGAAG ATCAGAAGTT GAACATGAAG 
GACAATGTGA GGCCCCTGCA GCCGCTGGGA AAACGCCAGG TGTTCAAGTC CCATGCCCCA 
GGACAGCTGC TGTCTTTGCC CCTGCCCACT CTGTTGGTCC CCACACTCAC CTGCCTGGTA 
GCCAACTTCC TCCAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_031633.1
CDS	134..1051
Translation	MQLLLALLALAYVAPSTEDSGWCYEIQTKDPRSSCLGPEKWPGACKENQQSPINIVTARTKVNPRLTPFILVGYDQKQQWPIKNNQHTVEMTLGGGACIIGGDLPARYEAVQLHLHWSNGNDNGSEHSIDGRHFAMEMHIVHKKLTSSKEDSKDKFAVLAFMIEVGDKVNKGFQPLVEALPSISKPHSTSTVRESSLQDMLPPSTKMYTYFRYNGSLTTPNCDETVIWTVYKQPIKIHKNQFLEFSKNLYYDEDQKLNMKDNVRPLQPLGKRQVFKSHAPGQLLSLPLPTLLVPTLTCLVANFLQ

Target ORF information:

RefSeq Version	NM_007607.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus carbonic anhydrase 4 (Car4), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_007607.2

ORF Insert Sequence:

ATGCAGCTCC TTCTTGCTCT GCTGGCGCTG GCATACGTGG CCCCCTCTAC TGAAGACTCA 
GGCTGGTGCT ATGAGATTCA AACCAAGGAT CCTAGAAGCA GTTGCTTGGG GCCTGAGAAG 
TGGCCTGGAG CCTGTAAGGA GAATCAGCAG TCCCCCATCA ACATTGTCAC TGCTAGGACA 
AAGGTGAACC CCAGACTGAC ACCCTTCATC CTCGTCGGCT ATGACCAAAA GCAACAGTGG 
CCAATTAAGA ACAATCAACA CACAGTGGAG ATGACGCTGG GGGGAGGTGC GTGCATTATC 
GGAGGAGACC TGCCCGCCCG GTACGAGGCT GTACAGTTGC ACCTGCACTG GTCAAATGGG 
AATGACAACG GTTCAGAGCA CAGTATTGAT GGGAGACACT TTGCCATGGA GATGCACATC 
GTGCACAAGA AGTTGACATC TAGCAAGGAG GACTCGAAGG ACAAGTTTGC AGTGCTGGCA 
TTTATGATTG AGGTAGGAGA CAAGGTGAAC AAGGGCTTCC AGCCCCTGGT GGAAGCACTG 
CCCAGTATCT CCAAACCCCA TTCAACCTCT ACAGTGAGGG AGAGCAGTTT GCAGGACATG 
CTTCCCCCGA GCACCAAAAT GTATACCTAC TTCCGTTACA ATGGCTCACT AACCACACCA 
AACTGTGATG AGACTGTCAT CTGGACTGTG TACAAACAAC CCATCAAGAT CCACAAAAAC 
CAGTTCCTGG AATTCTCAAA AAATCTGTAC TATGACGAAG ATCAGAAGTT GAACATGAAG 
GACAATGTGA GGCCCCTGCA GCCGCTGGGA AAACGCCAGG TGTTCAAGTC CCATGCCCCA 
GGACAGCTGC TGTCTTTGCC CCTGCCCACT CTGTTGGTCC CCACACTCAC CTGCCTGGTA 
GCCAACTTCC TCCAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Car4 ( XM_006532095.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006532158.1 Mus musculus carbonic anhydrase 4 (Car4), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu45490
Clone ID Related Accession (Same CDS sequence)	XM_006532095.3 , XM_006532095.4
Accession Version	XM_006532095.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 648bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	carbonic anhydrase 4 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000077.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006532095.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACGCTGG GGGGAGGTGC GTGCATTATC GGAGGAGACC TGCCCGCCCG GTACGAGGCT 
GTACAGTTGC ACCTGCACTG GTCAAATGGG AATGACAACG GTTCAGAGCA CAGTATTGAT 
GGGAGACACT TTGCCATGGA GATGCACATC GTGCACAAGA AGTTGACATC TAGCAAGGAG 
GACTCGAAGG ACAAGTTTGC AGTGCTGGCA TTTATGATTG AGGTAGGAGA CAAGGTGAAC 
AAGGGCTTCC AGCCCCTGGT GGAAGCACTG CCCAGTATCT CCAAACCCCA TTCAACCTCT 
ACAGTGAGGG AGAGCAGTTT GCAGGACATG CTTCCCCCGA GCACCAAAAT GTATACCTAC 
TTCCGTTACA ATGGCTCACT AACCACACCA AACTGTGATG AGACTGTCAT CTGGACTGTG 
TACAAACAAC CCATCAAGAT CCACAAAAAC CAGTTCCTGG AATTCTCAAA AAATCTGTAC 
TATGACGAAG ATCAGAAGTT GAACATGAAG GACAATGTGA GGCCCCTGCA GCCGCTGGGA 
AAACGCCAGG TGTTCAAGTC CCATGCCCCA GGACAGCTGC TGTCTTTGCC CCTGCCCACT 
CTGTTGGTCC CCACACTCAC CTGCCTGGTA GCCAACTTCC TCCAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006532158.1
CDS	538..1185
Translation	MTLGGGACIIGGDLPARYEAVQLHLHWSNGNDNGSEHSIDGRHFAMEMHIVHKKLTSSKEDSKDKFAVLAFMIEVGDKVNKGFQPLVEALPSISKPHSTSTVRESSLQDMLPPSTKMYTYFRYNGSLTTPNCDETVIWTVYKQPIKIHKNQFLEFSKNLYYDEDQKLNMKDNVRPLQPLGKRQVFKSHAPGQLLSLPLPTLLVPTLTCLVANFLQ

Target ORF information:

RefSeq Version	XM_006532095.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus carbonic anhydrase 4 (Car4), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006532095.3

ORF Insert Sequence:

ATGACGCTGG GGGGAGGTGC GTGCATTATC GGAGGAGACC TGCCCGCCCG GTACGAGGCT 
GTACAGTTGC ACCTGCACTG GTCAAATGGG AATGACAACG GTTCAGAGCA CAGTATTGAT 
GGGAGACACT TTGCCATGGA GATGCACATC GTGCACAAGA AGTTGACATC TAGCAAGGAG 
GACTCGAAGG ACAAGTTTGC AGTGCTGGCA TTTATGATTG AGGTAGGAGA CAAGGTGAAC 
AAGGGCTTCC AGCCCCTGGT GGAAGCACTG CCCAGTATCT CCAAACCCCA TTCAACCTCT 
ACAGTGAGGG AGAGCAGTTT GCAGGACATG CTTCCCCCGA GCACCAAAAT GTATACCTAC 
TTCCGTTACA ATGGCTCACT AACCACACCA AACTGTGATG AGACTGTCAT CTGGACTGTG 
TACAAACAAC CCATCAAGAT CCACAAAAAC CAGTTCCTGG AATTCTCAAA AAATCTGTAC 
TATGACGAAG ATCAGAAGTT GAACATGAAG GACAATGTGA GGCCCCTGCA GCCGCTGGGA 
AAACGCCAGG TGTTCAAGTC CCATGCCCCA GGACAGCTGC TGTCTTTGCC CCTGCCCACT 
CTGTTGGTCC CCACACTCAC CTGCCTGGTA GCCAACTTCC TCCAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Car4 ( XM_006532095.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006532158.1 Mus musculus carbonic anhydrase 4 (Car4), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu45490
Clone ID Related Accession (Same CDS sequence)	XM_006532095.3 , XM_006532095.4
Accession Version	XM_006532095.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 648bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	carbonic anhydrase 4 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000077.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006532095.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACGCTGG GGGGAGGTGC GTGCATTATC GGAGGAGACC TGCCCGCCCG GTACGAGGCT 
GTACAGTTGC ACCTGCACTG GTCAAATGGG AATGACAACG GTTCAGAGCA CAGTATTGAT 
GGGAGACACT TTGCCATGGA GATGCACATC GTGCACAAGA AGTTGACATC TAGCAAGGAG 
GACTCGAAGG ACAAGTTTGC AGTGCTGGCA TTTATGATTG AGGTAGGAGA CAAGGTGAAC 
AAGGGCTTCC AGCCCCTGGT GGAAGCACTG CCCAGTATCT CCAAACCCCA TTCAACCTCT 
ACAGTGAGGG AGAGCAGTTT GCAGGACATG CTTCCCCCGA GCACCAAAAT GTATACCTAC 
TTCCGTTACA ATGGCTCACT AACCACACCA AACTGTGATG AGACTGTCAT CTGGACTGTG 
TACAAACAAC CCATCAAGAT CCACAAAAAC CAGTTCCTGG AATTCTCAAA AAATCTGTAC 
TATGACGAAG ATCAGAAGTT GAACATGAAG GACAATGTGA GGCCCCTGCA GCCGCTGGGA 
AAACGCCAGG TGTTCAAGTC CCATGCCCCA GGACAGCTGC TGTCTTTGCC CCTGCCCACT 
CTGTTGGTCC CCACACTCAC CTGCCTGGTA GCCAACTTCC TCCAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006532158.1
CDS	718..1365
Translation	MTLGGGACIIGGDLPARYEAVQLHLHWSNGNDNGSEHSIDGRHFAMEMHIVHKKLTSSKEDSKDKFAVLAFMIEVGDKVNKGFQPLVEALPSISKPHSTSTVRESSLQDMLPPSTKMYTYFRYNGSLTTPNCDETVIWTVYKQPIKIHKNQFLEFSKNLYYDEDQKLNMKDNVRPLQPLGKRQVFKSHAPGQLLSLPLPTLLVPTLTCLVANFLQ

Target ORF information:

RefSeq Version	XM_006532095.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus carbonic anhydrase 4 (Car4), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006532095.4

ORF Insert Sequence:

ATGACGCTGG GGGGAGGTGC GTGCATTATC GGAGGAGACC TGCCCGCCCG GTACGAGGCT 
GTACAGTTGC ACCTGCACTG GTCAAATGGG AATGACAACG GTTCAGAGCA CAGTATTGAT 
GGGAGACACT TTGCCATGGA GATGCACATC GTGCACAAGA AGTTGACATC TAGCAAGGAG 
GACTCGAAGG ACAAGTTTGC AGTGCTGGCA TTTATGATTG AGGTAGGAGA CAAGGTGAAC 
AAGGGCTTCC AGCCCCTGGT GGAAGCACTG CCCAGTATCT CCAAACCCCA TTCAACCTCT 
ACAGTGAGGG AGAGCAGTTT GCAGGACATG CTTCCCCCGA GCACCAAAAT GTATACCTAC 
TTCCGTTACA ATGGCTCACT AACCACACCA AACTGTGATG AGACTGTCAT CTGGACTGTG 
TACAAACAAC CCATCAAGAT CCACAAAAAC CAGTTCCTGG AATTCTCAAA AAATCTGTAC 
TATGACGAAG ATCAGAAGTT GAACATGAAG GACAATGTGA GGCCCCTGCA GCCGCTGGGA 
AAACGCCAGG TGTTCAAGTC CCATGCCCCA GGACAGCTGC TGTCTTTGCC CCTGCCCACT 
CTGTTGGTCC CCACACTCAC CTGCCTGGTA GCCAACTTCC TCCAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

High-throughput discovery of novel developmental phenotypes.
Nature537(7621)508-514(2016 09)
Dickinson ME,Flenniken AM,Ji X,Teboul L,Wong MD,White JK,Meehan TF,Weninger WJ,Westerberg H,Adissu H,Baker CN,Bower L,Brown JM,Caddle LB,Chiani F,Clary D,Cleak J,Daly MJ,Denegre JM,Doe B,Dolan ME,Edie SM,Fuchs H,Gailus-Durner V,Galli A,Gambadoro A,Gallegos J,Guo S,Horner NR,Hsu CW,Johnson SJ,Kalaga S,Keith LC,Lanoue L,Lawson TN,Lek M,Mark M,Marschall S,Mason J,McElwee ML,Newbigging S,Nutter LM,Peterson KA,Ramirez-Solis R,Rowland DJ,Ryder E,Samocha KE,Seavitt JR,Selloum M,Szoke-Kovacs Z,Tamura M,Trainor AG,Tudose I,Wakana S,Warren J,Wendling O,West DB,Wong L,Yoshiki A,,,,,,,,,MacArthur DG,Tocchini-Valentini GP,Gao X,Flicek P,Bradley A,Skarnes WC,Justice MJ,Parkinson HE,Moore M,Wells S,Braun RE,Svenson KL,de Angelis MH,Herault Y,Mohun T,Mallon AM,Henkelman RM,Brown SD,Adams DJ,Lloyd KC,McKerlie C,Beaudet AL,Bu?an M,Murray SA

Bcl11b/Ctip2 is required for development of lingual papillae in mice.
Developmental biology416(1)98-110(2016 08)
Nishiguchi Y,Ohmoto M,Koki J,Enomoto T,Kominami R,Matsumoto I,Hirota J

Normal Fertility Requires the Expression of Carbonic Anhydrases II and IV in Sperm.
The Journal of biological chemistry290(49)29202-16(2015 Dec)
Wandernoth PM,Mannowetz N,Szczyrba J,Grannemann L,Wolf A,Becker HM,Sly WS,Wennemuth G

Thyroid hormone drives the expression of mouse carbonic anhydrase Car4 in kidney, lung and brain.
Molecular and cellular endocrinology41619-26(2015 Nov)
Vujovic M,Dudazy-Gralla S,H?rd J,Solsj? P,Warner A,Vennstr?m B,Mittag J

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Car4 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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