Aspm cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Aspm
Entrez Gene ID	12316
Full Name	abnormal spindle microtubule assembly
Synonyms	Calmbp1,D330028K02Rik,MCPH5,Sha1
General protein information	Preferred Names abnormal spindle microtubule assembly Names abnormal spindle-like microcephaly-associated protein homolog asp (abnormal spindle)-like, microcephaly associated calmodulin-binding protein 1 calmodulin-binding protein Sha1 spindle and hydroxyurea checkpoint abnormal protein
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 1 Map Location: 1\|1 E4-F

mRNA and Protein(s)

mRNA	Protein	Name
XM_017312957.2	XP_017168446.1	abnormal spindle-like microcephaly-associated protein homolog isoform X2
NM_009791.4	NP_033921.3	abnormal spindle-like microcephaly-associated protein homolog
XM_006529108.3	XP_006529171.1	abnormal spindle-like microcephaly-associated protein homolog isoform X1
XM_017312957.1	XP_017168446.1	abnormal spindle-like microcephaly-associated protein homolog isoform X2
XM_006529108.4	XP_006529171.1	abnormal spindle-like microcephaly-associated protein homolog isoform X1

Pathways from BioSystems

Homologies

Rattus norvegicus (Norway rat)	Aspm	NP_001099425.2
Bos taurus (cattle)	ASPM	NP_001193435.1
Mus musculus (house mouse)	Aspm	NP_033921.3
Danio rerio (zebrafish)	aspm	NP_001116845.1
Pan troglodytes (chimpanzee)	ASPM	NP_001008994.1
Gallus gallus (chicken)	ASPM	XP_422197.4
Xenopus tropicalis (tropical clawed frog)	aspm	XP_002932224.2
Homo sapiens (human)	ASPM	NP_060606.3
	ASPM	NP_001098005.1
Canis lupus familiaris (dog)	ASPM	NP_001184005.1

Gene Ontology
Bibliography

Related articles in PubMed

Longitudinal Diffusion Tensor Imaging Revealed Nerve Fiber Alterations in Aspm Mutated Microcephaly Model Mice.
Ogi H, Nitta N, Tando S, Fujimori A, Aoki I, Fushiki S, Itoh K
Neuroscience371325-336(2017 Dec)

Disruptions in asymmetric centrosome inheritance and WDR62-Aurora kinase B interactions in primary microcephaly.
Sgourdou P, Mishra-Gorur K, Saotome I, Henagariu O, Tuysuz B, Campos C, Ishigame K, Giannikou K, Quon JL, Sestan N, Caglayan AO, Gunel M, Louvi A
Scientific reports743708(2017 Mar)

Embryonic forebrain transcriptome of mice with polyalanine expansion mutations in the ARX homeobox gene.
Mattiske T, Lee K, Gecz J, Friocourt G, Shoubridge C
Human molecular genetics25(24)5433-5443(2016 12)

Microcephaly Proteins Wdr62 and Aspm Define a Mother Centriole Complex Regulating Centriole Biogenesis, Apical Complex, and Cell Fate.
Jayaraman D, Kodani A, Gonzalez DM, Mancias JD, Mochida GH, Vagnoni C, Johnson J, Krogan N, Harper JW, Reiter JF, Yu TW, Bae BI, Walsh CA
Neuron92(4)813-828(2016 Nov)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Aspm and Wdr62 interact genetically to control brain size, with mice lacking Wdr62, Aspm, or both showing gene dose-related centriole duplication defects that parallel the severity of the microcephaly and increased ectopic basal progenitors, suggesting premature delamination from the ventricular zone.
Title: Microcephaly Proteins Wdr62 and Aspm Define a Mother Centriole Complex Regulating Centriole Biogenesis, Apical Complex, and Cell Fate.

in this study we have provided evidence that ASPM controls spindle orientation by regulating the dynamics of astral MT and that CITK is a critical downstream partner of ASPM for this activity.
Title: ASPM and CITK regulate spindle orientation by affecting the dynamics of astral microtubules.

Interaction between ASPM and the Cdk2/Cyclin E complex regulated the Cyclin activity by modulating its ubiquitination and localization into the nucleus.
Title: ASPM regulates symmetric stem cell division by tuning Cyclin E ubiquitination.

Aspm, a gene that is mutated in familial microcephaly, regulates postnatal neurogenesis in the cerebellum and supports the growth of medulloblastoma, the most common malignant pediatric brain tumor.
Title: Aspm sustains postnatal cerebellar neurogenesis and medulloblastoma growth in mice.

Aspm is essential to the proliferation and differentiation of neural stem/progenitor cells. The Aspm gene loss model provided a novel pathogenetic insight into acquired microcephaly, which can be caused by in utero exposure teratogens.
Title: Disruption of Aspm causes microcephaly with abnormal neuronal differentiation.

The following Aspm gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Aspm cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu76951	XM_017312957.2 Latest version!	Mus musculus abnormal spindle microtubule assembly (Aspm), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OMu27884	XM_006529108.4 Latest version!	Mus musculus abnormal spindle microtubule assembly (Aspm), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OMu04856	NM_009791.4 Latest version!	Mus musculus abnormal spindle microtubule assembly (Aspm), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
View Old Accession Versions >>
OMu76951	XM_017312957.1	Mus musculus asp (abnormal spindle)-like, microcephaly associated (Drosophila) (Aspm), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OMu27884	XM_006529108.3	Mus musculus asp (abnormal spindle)-like, microcephaly associated (Drosophila) (Aspm), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Aspm ( XM_017312957.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017168446.1 Mus musculus abnormal spindle microtubule assembly (Aspm), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OMu76951
Clone ID Related Accession (Same CDS sequence)	XM_017312957.2 , XM_017312957.1
Accession Version	XM_017312957.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3795bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	abnormal spindle-like microcephaly-associated protein homolog isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_017312957.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGACGA TGCAGGCAGC CTCCTGCCCA GAGGAGAGGG GGCGGCGGGC GCGACCAGAT 
CCTGAGGCCG GGGACCCGTC TCCGCCGGTG CTGTTGCTCA GCCACTTCTG CGGCGTTCCC 
TTCCTCTGCT TCGGGGATGT CCGCGTGGGC ACGTCGCGGA CGCGGTCTCT GGTCCTGCAC 
AACCCGCACG AGGAACCTCT GCAGGTGGAG CTGTCGCTGC TGCGCGCCGC AGGCCAGGGC 
TTCAGCGTGG CGCCGAACCG CTGCGAGCTG AAGCCTAAAG AAAAACTTAC CATTTCCGTT 
ACCTGGACGC CACTGCGAGA AGGGGGAGTG AGGGAGATTG TCACATTTCT TGTAAATGAT 
TTCCTGAAGC ACCAGGCTAT ATTACTAGGA AATGCAGAAG AGCCTAAGAA GAAAAAGAGA 
AGTCTTTGGA ATACCAGTAA GAAGATTCCA GCCTCTTCAA AACATACAAA AAGGACTTCC 
AAAAACCAAC ATTTTAATGA ATCATTTACT ATTTCACAAA AAGACAGAAT TAGGAGCCCA 
CTGCAGCCTT GTGAAAATCT GGCTATGAGT GAATGCTCTT CCCCAACAGA AAACAAAGTC 
CCCACCCCAT CCATTAGTCC TATTAGAGAA TGCCAGAGTG AAACTTGCTT GCCACTGTTT 
TTACGCGAGT CCACTGCCTA TTCATCTCTT CATGAATCTG AAAATACACA AAATTTAAAA 
GTACAAGATG CCAGCATTTC ACAAACTTTT GATTTTAATG AGGAAGTCGC AAATGAAACT 
TTTATTAATC CCATTAGTGT CTGTCACCAG AGTGAAGGGG ATAGGAAACT CACGCTTGCC 
CCAAACTGTT CTTCACCTTT GAATAGTACA CAGACCCAAA TACACTTTCT AAGTCCAGAT 
TCTTTTGTAA ACAACAGGTA TACGTCTGAT AATGACCTGA AATCAATGAA AAATGTTTTA 
TCAGACACAT TTAGGAAAGA TCCCGCAGAG TCTGTATGTC TGGAATCCCA AACTGTCCAT 
GAAGTTTGCC AAACAATCTT AAGCCCAGAT TCTTTTTTAA ATGATAATTA TGGACTTAAA 
AAAGGGCTAA ACTTCAAGTC CGTCAATCCC GTTTTGTCGC CAACACAATT TGTAAAGGAT 
AGCATGGGAC ACGTAGGTCA GCAAACGGGT AAGTCAAATG AAGCTTCTCA GGATTGGAGA 
ATAAATGAAG GGTTGGCGTA TACCCCCGAG TGTCAGCATG CACAGACTCC CAGCAGCAGA 
TCTGAAAAAC AAAACCCTGT AGAAGTGAAG CCACATACGT ATGATTTTAC AAAGCAAAAG 
CCCAAGATTT CTGAGTTTCA GGATGCTTTT TGTCACCAGA GTAAACAACC GCACAAGAGA 
CGCCCAATTC TTTCTGCCAC TGTTACGAAA AGGAAACCCA CCAATGCCAG AGAAAAGCTA 
CCTGAGATTA ACAAGCCAGA CGCAAAAAGA TGTCTCGAAG GTCTAGTAGG TGAACGTGGA 
AAAGAAGTGG GTAGCCTGAG AGAGAAAGGT TTTCATTCTT CTCTTCCCGT TGTGGAGCCA 
GGAGTGAGTA AAGCTCTGAG CTACAGGGAT GAAGTAACGC CTGCAACTGT TGTCGTTGCT 
CGGAAAAGAA AGAGCCACGG AACAGTGGGA GATGCAAATG GAAAAGTAGC AGCTGAAGAA 
TGGATGGACA TGTGTGAAGT CAAACGAATC CATTTTTCTC CTCTGGAATC TACACCCTCA 
ACTGTTGCAA GAACCACAAA GAAAGAAGGG CATACCTCAA AGCGGATAAG CAGCCTGGAG 
CGGTCAGGCC TGAAGAAGAA AATGGATTCA TCAATATTAA AAACACCTCT TTCTAAGACA 
AAGAAAAAGA GAAGATCCAT TGTTGCTGTA GCACAGTCTC ATTTGACCTT TATAAAGCCA 
TTAAAAGCAG CTATTCCTAG ACACCCAATG CCGTTTGCTG CAAAGAACAT GTTCTATGAC 
GAACGCTGGA AGGAAAAGCA GGAACAGGGC TTCACTTGGT GGCTAAATTA TATATTAACA 
CCTGATGACT TCACTGTAAA AACCAATGTT TCTAAAGTGA ACGCTGCCAG CCTTGTTTTG 
GGAGCGGAAA GCCAACATAA AATAAGTGTC CCGAAAGCAC CTACTAAAGA GGAAGTGTCT 
CTCAGAGCTT ATACAGCCAG CTGCAGGCTG AATAGATTAC GCCGTACAGC TTGCTCCTTG 
TTTACTTCTG AAAAAATGGT TAAAGCTATT AAAAAGGTTG AAATCGAAAT TGAAGTTGGG 
CGGCTACTTG TTCGAAAAGA CCGACACCTC TGGAAAGATA TTGGACAACG CCAAAAAGTC 
CTGAATTGGC TCTTGTCATA TAATCCTCTG TGGCTTCGAA TTGGTCTGGA GACAGTTTTT 
GGAGAACTGA TCCCTCTGGC AGACAACAGT GACGTCACGG GATTGGCGAT GTTCATTCTG 
AATCGCTTAC TTTGGAATCC TGACATAGCA GCTGAATATA GACACCCCAC TGTTCCTCTC 
CTGTTTCGGG ATGGTCATGA AGCAGCCTTG TCTAAGTTCA CATTGAAAAA ATTGCTGTTG 
TTGATCTGTT TCCTTGATCA TGCTAAAATT TCCAGACTTA TTGATCATGA TCCTTGTCTC 
TTCTGTAAAG ATGCTGAATT CAAGGCTAGC AAAGAGCTTC TTCTAGCCTT TTCACGGGAT 
TTCCTCAGTG GTGAAGGTGA CCTTTCTCGT CACCTTAGCT TCTTGGGGTT ACCTGTGAGC 
CACGTTCAGA CACCACTTGA TGAATTTGAT TTTGCTGTTA CAAATCTTGC AGTGGACTTG 
CAGTGTGGGG TTCGCCTTGT GCGAACTGTG GAGCTTCTCA CACAGAACTG GAATCTGTCC 
GACAAACTCA GGATCCCTGC AATAAGTCGA GTTCAAAAGA TGCACAATGT TGATCTCGTC 
CTTCAGGTTC TCAAGTCTCG GGGAGTTCCA CTGACTGACG AGCATGGGAG CGCTATCTCG 
TCTAAGGATG TTGTGGACAG GCACAGAGAG AAAACCCTTG GGTTGCTTTG GAAAATAGCA 
CTGGCTTTCC AGGTGGATAT TTCCCTTAAT TTAGATCAAT TAAAAGAAGA AATTGATTTT 
CTAAAGCACA CACACAGTAT AAAGAGAGCT ATGTCTGCAC TAACATGCCC TTCACAAGCT 
ATTACTAATA AGCAAAGAGA CAAAAGGATT AGTGGGAACT TTGAACGCTA TGGTGACAGT 
GTGCAGTTGT TGATGGATTG GGTAAATGCA GTTTGTGCCT TCTATAATAA AAAGGTGGAG 
AATTTTACAG TGTCTTTCTC AGATGGCCGC ATACTATGCT ATTTGATCCA CCATTACCAC 
CCGTGCTATG TGCCTTTCGA TGCTATATGC CAGCGCACCA GTCAGTCCGT GGCCTGCGCA 
CAGACTGGTT CAGTGGTATT GAATTCTTCA TCGGAATCTG AAGGAGGTTG TCTGGATCTG 
TCTCTTGAAG CGCTTGATCA CGAAAGTACT CCAGAAATGT ACAAAGAGCT TCTAGAAAAC 
GAGAAGAAAA ATTTCCATTT GGTGCGATCT GCAGCTAGGG ATCTGGGAGG AATACCTGCT 
ATGATTCATC ATTCAGACAT GTCAAATACA ATTCCAGACG AAAAGGTGGT TATCACCTAT 
TTGTCATTTC TTTGTGCAAG GCTTTTGGAT CTCCGTAAAG AAATAAGAGC TGCTCGACTT 
ATACAGACAA CATGGAGAAA ATATAAACTA AAAAGAGATC TGAAACACCA TCAGGCCAGA 
GAGAACTTCT TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017168446.1
CDS	745..4539
Translation	MATMQAASCPEERGRRARPDPEAGDPSPPVLLLSHFCGVPFLCFGDVRVGTSRTRSLVLHNPHEEPLQVELSLLRAAGQGFSVAPNRCELKPKEKLTISVTWTPLREGGVREIVTFLVNDFLKHQAILLGNAEEPKKKKRSLWNTSKKIPASSKHTKRTSKNQHFNESFTISQKDRIRSPLQPCENLAMSECSSPTENKVPTPSISPIRECQSETCLPLFLRESTAYSSLHESENTQNLKVQDASISQTFDFNEEVANETFINPISVCHQSEGDRKLTLAPNCSSPLNSTQTQIHFLSPDSFVNNRYTSDNDLKSMKNVLSDTFRKDPAESVCLESQTVHEVCQTILSPDSFLNDNYGLKKGLNFKSVNPVLSPTQFVKDSMGHVGQQTGKSNEASQDWRINEGLAYTPECQHAQTPSSRSEKQNPVEVKPHTYDFTKQKPKISEFQDAFCHQSKQPHKRRPILSATVTKRKPTNAREKLPEINKPDAKRCLEGLVGERGKEVGSLREKGFHSSLPVVEPGVSKALSYRDEVTPATVVVARKRKSHGTVGDANGKVAAEEWMDMCEVKRIHFSPLESTPSTVARTTKKEGHTSKRISSLERSGLKKKMDSSILKTPLSKTKKKRRSIVAVAQSHLTFIKPLKAAIPRHPMPFAAKNMFYDERWKEKQEQGFTWWLNYILTPDDFTVKTNVSKVNAASLVLGAESQHKISVPKAPTKEEVSLRAYTASCRLNRLRRTACSLFTSEKMVKAIKKVEIEIEVGRLLVRKDRHLWKDIGQRQKVLNWLLSYNPLWLRIGLETVFGELIPLADNSDVTGLAMFILNRLLWNPDIAAEYRHPTVPLLFRDGHEAALSKFTLKKLLLLICFLDHAKISRLIDHDPCLFCKDAEFKASKELLLAFSRDFLSGEGDLSRHLSFLGLPVSHVQTPLDEFDFAVTNLAVDLQCGVRLVRTVELLTQNWNLSDKLRIPAISRVQKMHNVDLVLQVLKSRGVPLTDEHGSAISSKDVVDRHREKTLGLLWKIALAFQVDISLNLDQLKEEIDFLKHTHSIKRAMSALTCPSQAITNKQRDKRISGNFERYGDSVQLLMDWVNAVCAFYNKKVENFTVSFSDGRILCYLIHHYHPCYVPFDAICQRTSQSVACAQTGSVVLNSSSESEGGCLDLSLEALDHESTPEMYKELLENEKKNFHLVRSAARDLGGIPAMIHHSDMSNTIPDEKVVITYLSFLCARLLDLRKEIRAARLIQTTWRKYKLKRDLKHHQARENFL

Target ORF information:

RefSeq Version	XM_017312957.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus abnormal spindle microtubule assembly (Aspm), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017312957.2

ORF Insert Sequence:

ATGGCGACGA TGCAGGCAGC CTCCTGCCCA GAGGAGAGGG GGCGGCGGGC GCGACCAGAT 
CCTGAGGCCG GGGACCCGTC TCCGCCGGTG CTGTTGCTCA GCCACTTCTG CGGCGTTCCC 
TTCCTCTGCT TCGGGGATGT CCGCGTGGGC ACGTCGCGGA CGCGGTCTCT GGTCCTGCAC 
AACCCGCACG AGGAACCTCT GCAGGTGGAG CTGTCGCTGC TGCGCGCCGC AGGCCAGGGC 
TTCAGCGTGG CGCCGAACCG CTGCGAGCTG AAGCCTAAAG AAAAACTTAC CATTTCCGTT 
ACCTGGACGC CACTGCGAGA AGGGGGAGTG AGGGAGATTG TCACATTTCT TGTAAATGAT 
TTCCTGAAGC ACCAGGCTAT ATTACTAGGA AATGCAGAAG AGCCTAAGAA GAAAAAGAGA 
AGTCTTTGGA ATACCAGTAA GAAGATTCCA GCCTCTTCAA AACATACAAA AAGGACTTCC 
AAAAACCAAC ATTTTAATGA ATCATTTACT ATTTCACAAA AAGACAGAAT TAGGAGCCCA 
CTGCAGCCTT GTGAAAATCT GGCTATGAGT GAATGCTCTT CCCCAACAGA AAACAAAGTC 
CCCACCCCAT CCATTAGTCC TATTAGAGAA TGCCAGAGTG AAACTTGCTT GCCACTGTTT 
TTACGCGAGT CCACTGCCTA TTCATCTCTT CATGAATCTG AAAATACACA AAATTTAAAA 
GTACAAGATG CCAGCATTTC ACAAACTTTT GATTTTAATG AGGAAGTCGC AAATGAAACT 
TTTATTAATC CCATTAGTGT CTGTCACCAG AGTGAAGGGG ATAGGAAACT CACGCTTGCC 
CCAAACTGTT CTTCACCTTT GAATAGTACA CAGACCCAAA TACACTTTCT AAGTCCAGAT 
TCTTTTGTAA ACAACAGGTA TACGTCTGAT AATGACCTGA AATCAATGAA AAATGTTTTA 
TCAGACACAT TTAGGAAAGA TCCCGCAGAG TCTGTATGTC TGGAATCCCA AACTGTCCAT 
GAAGTTTGCC AAACAATCTT AAGCCCAGAT TCTTTTTTAA ATGATAATTA TGGACTTAAA 
AAAGGGCTAA ACTTCAAGTC CGTCAATCCC GTTTTGTCGC CAACACAATT TGTAAAGGAT 
AGCATGGGAC ACGTAGGTCA GCAAACGGGT AAGTCAAATG AAGCTTCTCA GGATTGGAGA 
ATAAATGAAG GGTTGGCGTA TACCCCCGAG TGTCAGCATG CACAGACTCC CAGCAGCAGA 
TCTGAAAAAC AAAACCCTGT AGAAGTGAAG CCACATACGT ATGATTTTAC AAAGCAAAAG 
CCCAAGATTT CTGAGTTTCA GGATGCTTTT TGTCACCAGA GTAAACAACC GCACAAGAGA 
CGCCCAATTC TTTCTGCCAC TGTTACGAAA AGGAAACCCA CCAATGCCAG AGAAAAGCTA 
CCTGAGATTA ACAAGCCAGA CGCAAAAAGA TGTCTCGAAG GTCTAGTAGG TGAACGTGGA 
AAAGAAGTGG GTAGCCTGAG AGAGAAAGGT TTTCATTCTT CTCTTCCCGT TGTGGAGCCA 
GGAGTGAGTA AAGCTCTGAG CTACAGGGAT GAAGTAACGC CTGCAACTGT TGTCGTTGCT 
CGGAAAAGAA AGAGCCACGG AACAGTGGGA GATGCAAATG GAAAAGTAGC AGCTGAAGAA 
TGGATGGACA TGTGTGAAGT CAAACGAATC CATTTTTCTC CTCTGGAATC TACACCCTCA 
ACTGTTGCAA GAACCACAAA GAAAGAAGGG CATACCTCAA AGCGGATAAG CAGCCTGGAG 
CGGTCAGGCC TGAAGAAGAA AATGGATTCA TCAATATTAA AAACACCTCT TTCTAAGACA 
AAGAAAAAGA GAAGATCCAT TGTTGCTGTA GCACAGTCTC ATTTGACCTT TATAAAGCCA 
TTAAAAGCAG CTATTCCTAG ACACCCAATG CCGTTTGCTG CAAAGAACAT GTTCTATGAC 
GAACGCTGGA AGGAAAAGCA GGAACAGGGC TTCACTTGGT GGCTAAATTA TATATTAACA 
CCTGATGACT TCACTGTAAA AACCAATGTT TCTAAAGTGA ACGCTGCCAG CCTTGTTTTG 
GGAGCGGAAA GCCAACATAA AATAAGTGTC CCGAAAGCAC CTACTAAAGA GGAAGTGTCT 
CTCAGAGCTT ATACAGCCAG CTGCAGGCTG AATAGATTAC GCCGTACAGC TTGCTCCTTG 
TTTACTTCTG AAAAAATGGT TAAAGCTATT AAAAAGGTTG AAATCGAAAT TGAAGTTGGG 
CGGCTACTTG TTCGAAAAGA CCGACACCTC TGGAAAGATA TTGGACAACG CCAAAAAGTC 
CTGAATTGGC TCTTGTCATA TAATCCTCTG TGGCTTCGAA TTGGTCTGGA GACAGTTTTT 
GGAGAACTGA TCCCTCTGGC AGACAACAGT GACGTCACGG GATTGGCGAT GTTCATTCTG 
AATCGCTTAC TTTGGAATCC TGACATAGCA GCTGAATATA GACACCCCAC TGTTCCTCTC 
CTGTTTCGGG ATGGTCATGA AGCAGCCTTG TCTAAGTTCA CATTGAAAAA ATTGCTGTTG 
TTGATCTGTT TCCTTGATCA TGCTAAAATT TCCAGACTTA TTGATCATGA TCCTTGTCTC 
TTCTGTAAAG ATGCTGAATT CAAGGCTAGC AAAGAGCTTC TTCTAGCCTT TTCACGGGAT 
TTCCTCAGTG GTGAAGGTGA CCTTTCTCGT CACCTTAGCT TCTTGGGGTT ACCTGTGAGC 
CACGTTCAGA CACCACTTGA TGAATTTGAT TTTGCTGTTA CAAATCTTGC AGTGGACTTG 
CAGTGTGGGG TTCGCCTTGT GCGAACTGTG GAGCTTCTCA CACAGAACTG GAATCTGTCC 
GACAAACTCA GGATCCCTGC AATAAGTCGA GTTCAAAAGA TGCACAATGT TGATCTCGTC 
CTTCAGGTTC TCAAGTCTCG GGGAGTTCCA CTGACTGACG AGCATGGGAG CGCTATCTCG 
TCTAAGGATG TTGTGGACAG GCACAGAGAG AAAACCCTTG GGTTGCTTTG GAAAATAGCA 
CTGGCTTTCC AGGTGGATAT TTCCCTTAAT TTAGATCAAT TAAAAGAAGA AATTGATTTT 
CTAAAGCACA CACACAGTAT AAAGAGAGCT ATGTCTGCAC TAACATGCCC TTCACAAGCT 
ATTACTAATA AGCAAAGAGA CAAAAGGATT AGTGGGAACT TTGAACGCTA TGGTGACAGT 
GTGCAGTTGT TGATGGATTG GGTAAATGCA GTTTGTGCCT TCTATAATAA AAAGGTGGAG 
AATTTTACAG TGTCTTTCTC AGATGGCCGC ATACTATGCT ATTTGATCCA CCATTACCAC 
CCGTGCTATG TGCCTTTCGA TGCTATATGC CAGCGCACCA GTCAGTCCGT GGCCTGCGCA 
CAGACTGGTT CAGTGGTATT GAATTCTTCA TCGGAATCTG AAGGAGGTTG TCTGGATCTG 
TCTCTTGAAG CGCTTGATCA CGAAAGTACT CCAGAAATGT ACAAAGAGCT TCTAGAAAAC 
GAGAAGAAAA ATTTCCATTT GGTGCGATCT GCAGCTAGGG ATCTGGGAGG AATACCTGCT 
ATGATTCATC ATTCAGACAT GTCAAATACA ATTCCAGACG AAAAGGTGGT TATCACCTAT 
TTGTCATTTC TTTGTGCAAG GCTTTTGGAT CTCCGTAAAG AAATAAGAGC TGCTCGACTT 
ATACAGACAA CATGGAGAAA ATATAAACTA AAAAGAGATC TGAAACACCA TCAGGCCAGA 
GAGAACTTCT TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Aspm ( XM_017312957.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017168446.1 Mus musculus asp (abnormal spindle)-like, microcephaly associated (Drosophila) (Aspm), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OMu76951
Clone ID Related Accession (Same CDS sequence)	XM_017312957.2 , XM_017312957.1
Accession Version	XM_017312957.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3795bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	abnormal spindle-like microcephaly-associated protein homolog isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGACGA TGCAGGCAGC CTCCTGCCCA GAGGAGAGGG GGCGGCGGGC GCGACCAGAT 
CCTGAGGCCG GGGACCCGTC TCCGCCGGTG CTGTTGCTCA GCCACTTCTG CGGCGTTCCC 
TTCCTCTGCT TCGGGGATGT CCGCGTGGGC ACGTCGCGGA CGCGGTCTCT GGTCCTGCAC 
AACCCGCACG AGGAACCTCT GCAGGTGGAG CTGTCGCTGC TGCGCGCCGC AGGCCAGGGC 
TTCAGCGTGG CGCCGAACCG CTGCGAGCTG AAGCCTAAAG AAAAACTTAC CATTTCCGTT 
ACCTGGACGC CACTGCGAGA AGGGGGAGTG AGGGAGATTG TCACATTTCT TGTAAATGAT 
TTCCTGAAGC ACCAGGCTAT ATTACTAGGA AATGCAGAAG AGCCTAAGAA GAAAAAGAGA 
AGTCTTTGGA ATACCAGTAA GAAGATTCCA GCCTCTTCAA AACATACAAA AAGGACTTCC 
AAAAACCAAC ATTTTAATGA ATCATTTACT ATTTCACAAA AAGACAGAAT TAGGAGCCCA 
CTGCAGCCTT GTGAAAATCT GGCTATGAGT GAATGCTCTT CCCCAACAGA AAACAAAGTC 
CCCACCCCAT CCATTAGTCC TATTAGAGAA TGCCAGAGTG AAACTTGCTT GCCACTGTTT 
TTACGCGAGT CCACTGCCTA TTCATCTCTT CATGAATCTG AAAATACACA AAATTTAAAA 
GTACAAGATG CCAGCATTTC ACAAACTTTT GATTTTAATG AGGAAGTCGC AAATGAAACT 
TTTATTAATC CCATTAGTGT CTGTCACCAG AGTGAAGGGG ATAGGAAACT CACGCTTGCC 
CCAAACTGTT CTTCACCTTT GAATAGTACA CAGACCCAAA TACACTTTCT AAGTCCAGAT 
TCTTTTGTAA ACAACAGGTA TACGTCTGAT AATGACCTGA AATCAATGAA AAATGTTTTA 
TCAGACACAT TTAGGAAAGA TCCCGCAGAG TCTGTATGTC TGGAATCCCA AACTGTCCAT 
GAAGTTTGCC AAACAATCTT AAGCCCAGAT TCTTTTTTAA ATGATAATTA TGGACTTAAA 
AAAGGGCTAA ACTTCAAGTC CGTCAATCCC GTTTTGTCGC CAACACAATT TGTAAAGGAT 
AGCATGGGAC ACGTAGGTCA GCAAACGGGT AAGTCAAATG AAGCTTCTCA GGATTGGAGA 
ATAAATGAAG GGTTGGCGTA TACCCCCGAG TGTCAGCATG CACAGACTCC CAGCAGCAGA 
TCTGAAAAAC AAAACCCTGT AGAAGTGAAG CCACATACGT ATGATTTTAC AAAGCAAAAG 
CCCAAGATTT CTGAGTTTCA GGATGCTTTT TGTCACCAGA GTAAACAACC GCACAAGAGA 
CGCCCAATTC TTTCTGCCAC TGTTACGAAA AGGAAACCCA CCAATGCCAG AGAAAAGCTA 
CCTGAGATTA ACAAGCCAGA CGCAAAAAGA TGTCTCGAAG GTCTAGTAGG TGAACGTGGA 
AAAGAAGTGG GTAGCCTGAG AGAGAAAGGT TTTCATTCTT CTCTTCCCGT TGTGGAGCCA 
GGAGTGAGTA AAGCTCTGAG CTACAGGGAT GAAGTAACGC CTGCAACTGT TGTCGTTGCT 
CGGAAAAGAA AGAGCCACGG AACAGTGGGA GATGCAAATG GAAAAGTAGC AGCTGAAGAA 
TGGATGGACA TGTGTGAAGT CAAACGAATC CATTTTTCTC CTCTGGAATC TACACCCTCA 
ACTGTTGCAA GAACCACAAA GAAAGAAGGG CATACCTCAA AGCGGATAAG CAGCCTGGAG 
CGGTCAGGCC TGAAGAAGAA AATGGATTCA TCAATATTAA AAACACCTCT TTCTAAGACA 
AAGAAAAAGA GAAGATCCAT TGTTGCTGTA GCACAGTCTC ATTTGACCTT TATAAAGCCA 
TTAAAAGCAG CTATTCCTAG ACACCCAATG CCGTTTGCTG CAAAGAACAT GTTCTATGAC 
GAACGCTGGA AGGAAAAGCA GGAACAGGGC TTCACTTGGT GGCTAAATTA TATATTAACA 
CCTGATGACT TCACTGTAAA AACCAATGTT TCTAAAGTGA ACGCTGCCAG CCTTGTTTTG 
GGAGCGGAAA GCCAACATAA AATAAGTGTC CCGAAAGCAC CTACTAAAGA GGAAGTGTCT 
CTCAGAGCTT ATACAGCCAG CTGCAGGCTG AATAGATTAC GCCGTACAGC TTGCTCCTTG 
TTTACTTCTG AAAAAATGGT TAAAGCTATT AAAAAGGTTG AAATCGAAAT TGAAGTTGGG 
CGGCTACTTG TTCGAAAAGA CCGACACCTC TGGAAAGATA TTGGACAACG CCAAAAAGTC 
CTGAATTGGC TCTTGTCATA TAATCCTCTG TGGCTTCGAA TTGGTCTGGA GACAGTTTTT 
GGAGAACTGA TCCCTCTGGC AGACAACAGT GACGTCACGG GATTGGCGAT GTTCATTCTG 
AATCGCTTAC TTTGGAATCC TGACATAGCA GCTGAATATA GACACCCCAC TGTTCCTCTC 
CTGTTTCGGG ATGGTCATGA AGCAGCCTTG TCTAAGTTCA CATTGAAAAA ATTGCTGTTG 
TTGATCTGTT TCCTTGATCA TGCTAAAATT TCCAGACTTA TTGATCATGA TCCTTGTCTC 
TTCTGTAAAG ATGCTGAATT CAAGGCTAGC AAAGAGCTTC TTCTAGCCTT TTCACGGGAT 
TTCCTCAGTG GTGAAGGTGA CCTTTCTCGT CACCTTAGCT TCTTGGGGTT ACCTGTGAGC 
CACGTTCAGA CACCACTTGA TGAATTTGAT TTTGCTGTTA CAAATCTTGC AGTGGACTTG 
CAGTGTGGGG TTCGCCTTGT GCGAACTGTG GAGCTTCTCA CACAGAACTG GAATCTGTCC 
GACAAACTCA GGATCCCTGC AATAAGTCGA GTTCAAAAGA TGCACAATGT TGATCTCGTC 
CTTCAGGTTC TCAAGTCTCG GGGAGTTCCA CTGACTGACG AGCATGGGAG CGCTATCTCG 
TCTAAGGATG TTGTGGACAG GCACAGAGAG AAAACCCTTG GGTTGCTTTG GAAAATAGCA 
CTGGCTTTCC AGGTGGATAT TTCCCTTAAT TTAGATCAAT TAAAAGAAGA AATTGATTTT 
CTAAAGCACA CACACAGTAT AAAGAGAGCT ATGTCTGCAC TAACATGCCC TTCACAAGCT 
ATTACTAATA AGCAAAGAGA CAAAAGGATT AGTGGGAACT TTGAACGCTA TGGTGACAGT 
GTGCAGTTGT TGATGGATTG GGTAAATGCA GTTTGTGCCT TCTATAATAA AAAGGTGGAG 
AATTTTACAG TGTCTTTCTC AGATGGCCGC ATACTATGCT ATTTGATCCA CCATTACCAC 
CCGTGCTATG TGCCTTTCGA TGCTATATGC CAGCGCACCA GTCAGTCCGT GGCCTGCGCA 
CAGACTGGTT CAGTGGTATT GAATTCTTCA TCGGAATCTG AAGGAGGTTG TCTGGATCTG 
TCTCTTGAAG CGCTTGATCA CGAAAGTACT CCAGAAATGT ACAAAGAGCT TCTAGAAAAC 
GAGAAGAAAA ATTTCCATTT GGTGCGATCT GCAGCTAGGG ATCTGGGAGG AATACCTGCT 
ATGATTCATC ATTCAGACAT GTCAAATACA ATTCCAGACG AAAAGGTGGT TATCACCTAT 
TTGTCATTTC TTTGTGCAAG GCTTTTGGAT CTCCGTAAAG AAATAAGAGC TGCTCGACTT 
ATACAGACAA CATGGAGAAA ATATAAACTA AAAAGAGATC TGAAACACCA TCAGGCCAGA 
GAGAACTTCT TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017168446.1
CDS	900..4694
Translation	MATMQAASCPEERGRRARPDPEAGDPSPPVLLLSHFCGVPFLCFGDVRVGTSRTRSLVLHNPHEEPLQVELSLLRAAGQGFSVAPNRCELKPKEKLTISVTWTPLREGGVREIVTFLVNDFLKHQAILLGNAEEPKKKKRSLWNTSKKIPASSKHTKRTSKNQHFNESFTISQKDRIRSPLQPCENLAMSECSSPTENKVPTPSISPIRECQSETCLPLFLRESTAYSSLHESENTQNLKVQDASISQTFDFNEEVANETFINPISVCHQSEGDRKLTLAPNCSSPLNSTQTQIHFLSPDSFVNNRYTSDNDLKSMKNVLSDTFRKDPAESVCLESQTVHEVCQTILSPDSFLNDNYGLKKGLNFKSVNPVLSPTQFVKDSMGHVGQQTGKSNEASQDWRINEGLAYTPECQHAQTPSSRSEKQNPVEVKPHTYDFTKQKPKISEFQDAFCHQSKQPHKRRPILSATVTKRKPTNAREKLPEINKPDAKRCLEGLVGERGKEVGSLREKGFHSSLPVVEPGVSKALSYRDEVTPATVVVARKRKSHGTVGDANGKVAAEEWMDMCEVKRIHFSPLESTPSTVARTTKKEGHTSKRISSLERSGLKKKMDSSILKTPLSKTKKKRRSIVAVAQSHLTFIKPLKAAIPRHPMPFAAKNMFYDERWKEKQEQGFTWWLNYILTPDDFTVKTNVSKVNAASLVLGAESQHKISVPKAPTKEEVSLRAYTASCRLNRLRRTACSLFTSEKMVKAIKKVEIEIEVGRLLVRKDRHLWKDIGQRQKVLNWLLSYNPLWLRIGLETVFGELIPLADNSDVTGLAMFILNRLLWNPDIAAEYRHPTVPLLFRDGHEAALSKFTLKKLLLLICFLDHAKISRLIDHDPCLFCKDAEFKASKELLLAFSRDFLSGEGDLSRHLSFLGLPVSHVQTPLDEFDFAVTNLAVDLQCGVRLVRTVELLTQNWNLSDKLRIPAISRVQKMHNVDLVLQVLKSRGVPLTDEHGSAISSKDVVDRHREKTLGLLWKIALAFQVDISLNLDQLKEEIDFLKHTHSIKRAMSALTCPSQAITNKQRDKRISGNFERYGDSVQLLMDWVNAVCAFYNKKVENFTVSFSDGRILCYLIHHYHPCYVPFDAICQRTSQSVACAQTGSVVLNSSSESEGGCLDLSLEALDHESTPEMYKELLENEKKNFHLVRSAARDLGGIPAMIHHSDMSNTIPDEKVVITYLSFLCARLLDLRKEIRAARLIQTTWRKYKLKRDLKHHQARENFL

Target ORF information:

RefSeq Version	XM_017312957.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus asp (abnormal spindle)-like, microcephaly associated (Drosophila) (Aspm), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017312957.1

ORF Insert Sequence:

ATGGCGACGA TGCAGGCAGC CTCCTGCCCA GAGGAGAGGG GGCGGCGGGC GCGACCAGAT 
CCTGAGGCCG GGGACCCGTC TCCGCCGGTG CTGTTGCTCA GCCACTTCTG CGGCGTTCCC 
TTCCTCTGCT TCGGGGATGT CCGCGTGGGC ACGTCGCGGA CGCGGTCTCT GGTCCTGCAC 
AACCCGCACG AGGAACCTCT GCAGGTGGAG CTGTCGCTGC TGCGCGCCGC AGGCCAGGGC 
TTCAGCGTGG CGCCGAACCG CTGCGAGCTG AAGCCTAAAG AAAAACTTAC CATTTCCGTT 
ACCTGGACGC CACTGCGAGA AGGGGGAGTG AGGGAGATTG TCACATTTCT TGTAAATGAT 
TTCCTGAAGC ACCAGGCTAT ATTACTAGGA AATGCAGAAG AGCCTAAGAA GAAAAAGAGA 
AGTCTTTGGA ATACCAGTAA GAAGATTCCA GCCTCTTCAA AACATACAAA AAGGACTTCC 
AAAAACCAAC ATTTTAATGA ATCATTTACT ATTTCACAAA AAGACAGAAT TAGGAGCCCA 
CTGCAGCCTT GTGAAAATCT GGCTATGAGT GAATGCTCTT CCCCAACAGA AAACAAAGTC 
CCCACCCCAT CCATTAGTCC TATTAGAGAA TGCCAGAGTG AAACTTGCTT GCCACTGTTT 
TTACGCGAGT CCACTGCCTA TTCATCTCTT CATGAATCTG AAAATACACA AAATTTAAAA 
GTACAAGATG CCAGCATTTC ACAAACTTTT GATTTTAATG AGGAAGTCGC AAATGAAACT 
TTTATTAATC CCATTAGTGT CTGTCACCAG AGTGAAGGGG ATAGGAAACT CACGCTTGCC 
CCAAACTGTT CTTCACCTTT GAATAGTACA CAGACCCAAA TACACTTTCT AAGTCCAGAT 
TCTTTTGTAA ACAACAGGTA TACGTCTGAT AATGACCTGA AATCAATGAA AAATGTTTTA 
TCAGACACAT TTAGGAAAGA TCCCGCAGAG TCTGTATGTC TGGAATCCCA AACTGTCCAT 
GAAGTTTGCC AAACAATCTT AAGCCCAGAT TCTTTTTTAA ATGATAATTA TGGACTTAAA 
AAAGGGCTAA ACTTCAAGTC CGTCAATCCC GTTTTGTCGC CAACACAATT TGTAAAGGAT 
AGCATGGGAC ACGTAGGTCA GCAAACGGGT AAGTCAAATG AAGCTTCTCA GGATTGGAGA 
ATAAATGAAG GGTTGGCGTA TACCCCCGAG TGTCAGCATG CACAGACTCC CAGCAGCAGA 
TCTGAAAAAC AAAACCCTGT AGAAGTGAAG CCACATACGT ATGATTTTAC AAAGCAAAAG 
CCCAAGATTT CTGAGTTTCA GGATGCTTTT TGTCACCAGA GTAAACAACC GCACAAGAGA 
CGCCCAATTC TTTCTGCCAC TGTTACGAAA AGGAAACCCA CCAATGCCAG AGAAAAGCTA 
CCTGAGATTA ACAAGCCAGA CGCAAAAAGA TGTCTCGAAG GTCTAGTAGG TGAACGTGGA 
AAAGAAGTGG GTAGCCTGAG AGAGAAAGGT TTTCATTCTT CTCTTCCCGT TGTGGAGCCA 
GGAGTGAGTA AAGCTCTGAG CTACAGGGAT GAAGTAACGC CTGCAACTGT TGTCGTTGCT 
CGGAAAAGAA AGAGCCACGG AACAGTGGGA GATGCAAATG GAAAAGTAGC AGCTGAAGAA 
TGGATGGACA TGTGTGAAGT CAAACGAATC CATTTTTCTC CTCTGGAATC TACACCCTCA 
ACTGTTGCAA GAACCACAAA GAAAGAAGGG CATACCTCAA AGCGGATAAG CAGCCTGGAG 
CGGTCAGGCC TGAAGAAGAA AATGGATTCA TCAATATTAA AAACACCTCT TTCTAAGACA 
AAGAAAAAGA GAAGATCCAT TGTTGCTGTA GCACAGTCTC ATTTGACCTT TATAAAGCCA 
TTAAAAGCAG CTATTCCTAG ACACCCAATG CCGTTTGCTG CAAAGAACAT GTTCTATGAC 
GAACGCTGGA AGGAAAAGCA GGAACAGGGC TTCACTTGGT GGCTAAATTA TATATTAACA 
CCTGATGACT TCACTGTAAA AACCAATGTT TCTAAAGTGA ACGCTGCCAG CCTTGTTTTG 
GGAGCGGAAA GCCAACATAA AATAAGTGTC CCGAAAGCAC CTACTAAAGA GGAAGTGTCT 
CTCAGAGCTT ATACAGCCAG CTGCAGGCTG AATAGATTAC GCCGTACAGC TTGCTCCTTG 
TTTACTTCTG AAAAAATGGT TAAAGCTATT AAAAAGGTTG AAATCGAAAT TGAAGTTGGG 
CGGCTACTTG TTCGAAAAGA CCGACACCTC TGGAAAGATA TTGGACAACG CCAAAAAGTC 
CTGAATTGGC TCTTGTCATA TAATCCTCTG TGGCTTCGAA TTGGTCTGGA GACAGTTTTT 
GGAGAACTGA TCCCTCTGGC AGACAACAGT GACGTCACGG GATTGGCGAT GTTCATTCTG 
AATCGCTTAC TTTGGAATCC TGACATAGCA GCTGAATATA GACACCCCAC TGTTCCTCTC 
CTGTTTCGGG ATGGTCATGA AGCAGCCTTG TCTAAGTTCA CATTGAAAAA ATTGCTGTTG 
TTGATCTGTT TCCTTGATCA TGCTAAAATT TCCAGACTTA TTGATCATGA TCCTTGTCTC 
TTCTGTAAAG ATGCTGAATT CAAGGCTAGC AAAGAGCTTC TTCTAGCCTT TTCACGGGAT 
TTCCTCAGTG GTGAAGGTGA CCTTTCTCGT CACCTTAGCT TCTTGGGGTT ACCTGTGAGC 
CACGTTCAGA CACCACTTGA TGAATTTGAT TTTGCTGTTA CAAATCTTGC AGTGGACTTG 
CAGTGTGGGG TTCGCCTTGT GCGAACTGTG GAGCTTCTCA CACAGAACTG GAATCTGTCC 
GACAAACTCA GGATCCCTGC AATAAGTCGA GTTCAAAAGA TGCACAATGT TGATCTCGTC 
CTTCAGGTTC TCAAGTCTCG GGGAGTTCCA CTGACTGACG AGCATGGGAG CGCTATCTCG 
TCTAAGGATG TTGTGGACAG GCACAGAGAG AAAACCCTTG GGTTGCTTTG GAAAATAGCA 
CTGGCTTTCC AGGTGGATAT TTCCCTTAAT TTAGATCAAT TAAAAGAAGA AATTGATTTT 
CTAAAGCACA CACACAGTAT AAAGAGAGCT ATGTCTGCAC TAACATGCCC TTCACAAGCT 
ATTACTAATA AGCAAAGAGA CAAAAGGATT AGTGGGAACT TTGAACGCTA TGGTGACAGT 
GTGCAGTTGT TGATGGATTG GGTAAATGCA GTTTGTGCCT TCTATAATAA AAAGGTGGAG 
AATTTTACAG TGTCTTTCTC AGATGGCCGC ATACTATGCT ATTTGATCCA CCATTACCAC 
CCGTGCTATG TGCCTTTCGA TGCTATATGC CAGCGCACCA GTCAGTCCGT GGCCTGCGCA 
CAGACTGGTT CAGTGGTATT GAATTCTTCA TCGGAATCTG AAGGAGGTTG TCTGGATCTG 
TCTCTTGAAG CGCTTGATCA CGAAAGTACT CCAGAAATGT ACAAAGAGCT TCTAGAAAAC 
GAGAAGAAAA ATTTCCATTT GGTGCGATCT GCAGCTAGGG ATCTGGGAGG AATACCTGCT 
ATGATTCATC ATTCAGACAT GTCAAATACA ATTCCAGACG AAAAGGTGGT TATCACCTAT 
TTGTCATTTC TTTGTGCAAG GCTTTTGGAT CTCCGTAAAG AAATAAGAGC TGCTCGACTT 
ATACAGACAA CATGGAGAAA ATATAAACTA AAAAGAGATC TGAAACACCA TCAGGCCAGA 
GAGAACTTCT TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Aspm ( XM_006529108.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006529171.1 Mus musculus abnormal spindle microtubule assembly (Aspm), transcript variant X1, mRNA.

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CloneID	OMu27884
Clone ID Related Accession (Same CDS sequence)	XM_006529108.3 , XM_006529108.4
Accession Version	XM_006529108.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5706bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	abnormal spindle-like microcephaly-associated protein homolog isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006529108.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGATGA CAGCATCTAT CTGTATCTTC CTAAGTGGGA ACATATGCAG CACCTCTCTA 
GATCCTCATA CGCATTTCCT GAGCTGCCAG GCTGATAAGG ATCATCCTGA GGAAAGAGAC 
AAAGCTGCTA GAGTTATTCA GTCAGTTGTT CTCAATTTTC TAAGTAGACG AAGATTGCAA 
AAAAACGTCA GTGCAGCTCT GGTCATTCAG AAATGCTGGC GAAGAGTCTC AGCGCAAAGG 
AAGTTGCGGA TGCTGAAAAA TGAAAAACTG GCAAAACTTC AAAATAAATC AGCAGTGCTC 
ATTCAGGCTT ATTGGAGAAG ATACTCCACC AGAAAACGGT TTCTGAGGTT GAAGCACTAT 
TCAGTCATCC TTCAGTCTAG GATAAGAATG AAAATTGCTC TTACATCTTA CAAACGATAT 
CTTTGGGCCA CAGTTACAAT TCAGAGGCAT TGGCGTGCCT ATTTAAGTAG AAAACGAGAT 
CAGCAAATAT TTAGGAAGCT GAAATCCTCA TCCCTTGTAA TCCAGTTTAT GTTCAGAAGG 
TGGAAGCGAC GCAAGCTGCA GTTACAAACC AAAGCTGCAG TAACATTGCA GCGAGCTTTT 
AGAGAATGGC ATCTCAGGAA ACAAATACGA GAAAGATCCG CTGTTGTGAT ACAGTCATGG 
TACAGAATGC ACAGGGAATT GCAGAAATAC ATTTATATTA GGTCTTGTGT TATTGTCATC 
CAGAGAAGAG TTCGGTGCTT TCAAGCCCAA AAGTTATATA AGAGAAGGAA AGATGCCATC 
CTGACTCTCC AGAAACATTA CAGGGCACGT CAGAAGGGGA AGCTAGCACA CGCTGACTAT 
TTGCAGAAAC GAGCTGCCAC CATTCGACTG CAGGCTGCAT TCAGGGGGAT GAAGGCTCGC 
CATTCATACA GGTTGCAGAT TGGAGCTGCC TGTGTTCTTC AGTCCTATTG GAGAATGAGA 
CAGGAAAGAG TTAGATTTTT AAACCTTAAA AAGATGGTTA TTAAGTTGCA AGCACACATA 
AGAAAATACC AACAACTACA GAAATATAAG AAGATAAAGA AAGCAGCCAT TACAATTCAG 
ACTCATTTCC GAGCTTCCAT TTCAGCCAGG AGGGTTCTAG CATCTTATCA GAAAACAAGG 
TCTTCAGTCA TTGTTCTACA GTCTGCATGT AGAGGGATGC AAGCCAGAAA AGCATTTCGC 
CATGCTTTAG CATCCGTTAT AAAGATTCAG TCATATTATC GGGCTTATAT ATGTAGGAAG 
ACATTTCAGA ACTTCAAAAA TGCCACAATA AAGCTGCAGT CAATTGTCAA AATGAAACAA 
TCACGGAAGC AGTATTTACA AATAAGAGCA GCTGCACTGT TTATCCAGCG GTGGTACCGT 
TCCCAAAAAC TGGCCTCACA AAAGAGAAAA GAGTACATAC AGGTGCGTGA ATCCTGTATC 
AAACTGCAGT CACACTTTAG AGGATGCCTG GTCAGAAAGC AGTTGAGGTT GCAATGTAAA 
GCTGCCATTT CTCTACAGTC TTATTTCAGA ATGAGAACAG CGAGGCAGCG CTACCTGAAG 
ATGTGCAAGG CGGCTCTTGT CATTCAGAGT TTCTACTGTG CCTACAGAGC ACAAATCAGT 
CAAAGGAAGA ACTTCTTGCA GGTCAAAAGA GCAGCTATTT GTTTGCAGGC AGCTTACAGA 
GGCTGCAAAG TACGCCGGCA AATCAAGCAG CAGTCTACGG CTGCTGTTAC AATTCAGAGG 
GTTTTTAGAG GCCACAGTCA GAGGATGAAA TACCAGACTA TGCTTCAGTC TGCTGTGAAG 
ATTCAGAGAT GGTACAGGGC ACAGAAGGTT GCTTATGACA TGAGAATTCA ATTTCTAAAG 
ACTAGAGAGG CTGTGGTTTG CCTCCAGTCT GCCTACCGTG GCTGGCAGGT TCGGCAACAG 
CTCAGGAGGC AACATGAGGC TGCAGTGAAG ATCCAGTCTA CGTTTAGAAT GGCCGTGGCC 
CAGCAACAGT ATAAACTACT AAGAGCAGCT GCAGCAGTCA TCCAGCAGCA TGTGAGGGCA 
CGGGCTGCAG GGAAGAGGCA GCACTTGGCG TACATCCAGC TCCGTCATGC AGCTCTGGTG 
TTTCAGGCTG CATGGAAGGG AAAGATGCTG AGAAGACAGA TTGCCCGGCA GCATCAATGC 
GCTGCCCTCA TACAGTCATA TTACAGGATG CACATTCAGC GCAGGAAGTG GAGCATCATG 
AAAACCGCTG CCCTTCAGAT TCAGCTGTGC TATAGGGCTT ACAAAGTTGG AAAAGAACAG 
CGCCACTTAT ACTTGAAAAC AAAAGCAGCT GTAGTAACTT TACAGTCAGC TTACCGTGGT 
ATGAAAGTGA GAAAGCGGGT AGCTGAATGC CACAAAGCAG CGGTCACTAT ACAGTCTAAA 
TTTAGAGCCT ACAGAACACA AAAGAAGTAC ACGACCTATA GAACTTCAGC TATTGTAATT 
CAGAGGTGGT ACCGGAATAT TAAAATTACA ACCCAGCAAC ATCAGGAATA CCTTAACTTA 
AGGAGGGCAG CAGTTCAAGT CCAGGCTGCG TATAGAGGCA TTAGAGTGAG GAGACGCATT 
CAACACATGC ACATGGCGGC CACACTTATT GAAGCCATGT TTAAGATGCG TCAGTCAAGA 
GTAAGGTACC TCAAGATGAG AACAGCAGCT CTTATAATTC AGGTGAGATA CAGAGCGTAT 
TATCTAGGTA AGATTCAGCA TGAGAAGTAC CTGAGGACTT TGAAGGCTAT TAAAACCCTT 
CAAGCTGGAG TCAGAGGAGC AAGAGTGAGG CGGACTGTAA GAAAGATGCA TTTCGCAGCA 
ACACTCATTC AGTCACATTT TAGGGGACAC AGGCAGCAAA CTTACTTTCA TCGGTTGAGG 
AAGGCGGCAA CCATGGTGCA GCAGAGATAC CGAGCAGTGA AAGAAGGAAG CGCTGAGTTT 
CAGAGGTACA GCAGACTAAG GCGTTCTGTA CTCCTCATCC AGGCTGCCTT CAGGGGACTG 
AGAACTAGGA GACACTTAAA AGCTATGCAC CTGGCTGCTA CTCTCATTCA GAGGAGATTC 
AGGACTTTCG CGATGAGAAG GAAGTTCCTT TCCCTCAGGA AAACTGCCAT TTGGATTCAG 
AGACAGTACC GTGCAAGACT TTATGCCAAG TATTCCCGAC AACAGTTACT GTTGGAAAAG 
GCAGTTATAA AGATCCAGTC ATCATACAGA GGATGGGTGG TAAGGAAAAG GGTGCAAAAG 
ATGCACAGGG CGGCTACTGT GATTCAGGCC ACTTTCCGGA TGCACGGAGC CTATATGAGG 
TACCAGCATT TGAAGCGGGC CTCAGTTGTG ATCCAAGTAC ACACAGCTGC AGAACTGCAG 
AGGCAAAAGC ATGCTGCTGT AATCCTTCAG GCTGCCGTTA GGGGGATGAA AACCAGAAGT 
CATCTGAAGA CTATGCATTC TTCTGCAACC CTTATTCAGA GTCAATTTAG AGCTTTTATA 
GTAAGGAGAC GATTCATTGC TCTCAGAAAA GCTGCTATAT TTGTGCAGAG GAAATTCCGA 
GCCACCCTTT ATGCCAAGCA TAAATTGCAC CAATTCTTGC AGTTACGAAA GGCAGCCATT 
ACCATACAGT CCTCTTATCG AAGACTGATG GTACAAAAGA AGTTACAAGA AATGCACAGG 
GCTGCAGCTC TCATCCAGGC TACTTTTAGA ATGCACAGAA CTTACGTTGC ATTTCACATT 
TGGAAATGTG CCTCGATTAG AATTCAGCAG TGTTATCGAA CATACAGAAC TATAAAATTA 
CAAAAAGAAA AACTTATCAG AGAAGAACAA CACAGTGCTG CTGTCCTAAT ACAAAGCACG 
TACAGGATGT ACAGGCAGCG TTGCTTCTAT CAACAGCGTC GGTGGGCAGC AAAGGTAATA 
CAAAAAACAT ACAGAGCAAA TAAAAGACGG CAAGATCTGC TCTATGTGTG TAAGGAAGAA 
ACACCTCTTC TTCAGATGCA TTTTCAGGGT TTAAACACTG CAAAACAAGG TCGGCAGCAG 
CATGGGGCCG CTATGATTAC ACAGAAGCAT TTTAGAGCCT TTAAAGCAAG GAGGCTGATG 
GAGGCCGAGA GAGGCTTCCA GGCTGGATGT AGAAAATATA AAGCTAAGAA ATACTTATCC 
AAAGTGGAAG CTGCCTGCAG GATTCAAGCT TGGTATAGGC GTTGGAGAGC GCACAAGAAA 
TATCTAACTT TATTGAAAGC TGTTAACATT ATTGAAGGTT ACCTCTCTGC ACAGCTGGCG 
AGAAGGAGGT TCTTGAAGAT GAGGGCGGCA GCTATTATAA TTCAAAGGAA ATGGAGAGCT 
ACACTTTCTG TAAGAGGAGC TCGTGAGAAT TTAAAAAGAC ATCGAGCTGC TTGTGTGATT 
CAAGCCCACT TTAGGGGCTA TCAGGCAAGG CAGTCCTTCC TGCAGCAGAG ATCTGCTGTT 
TTAATCATAC AGAGACATGT ACGAGCCATG GTGGCTGCGA AGCAGGAAAG GATAAAGTAT 
ATCAAATTGA AAAAGTCTAC GGTTGTTGTA CAAGCCTTGG TGCGTGGCTG GTTAGTAAGG 
AAAAGGGTTT CAGAGCAGAA AGCCAAAACT CGGCTTTTCC ACTTTACAGC AGCTGCCTAC 
TGTCACATGT GTGCTCTTAA AATCCAACGA GCTTATCGGC TCCACGTGAC CTTGAGGAAC 
GCTAAGAAGC ACATGGATTC AGTCATCTTC ATTCAGAGAT GGTTTCGAAA AAGATTACAA 
CGAAAGAGGT TCATTGAACA GTACCACAAG ATCCTAAGCA CTCGACGTGA AGCTCATGCA 
TGTTGGCTCC AGCAGGACAG GGCTGCATCC GTGATACAGA AGGCGGTACG CCGCTTTCTC 
CTCTGCAGAA GACAGGAAAA GATCACTAGC TGCGCCACTA GAATTCAGGC ATTATGGAGA 
GGCTATTCTT GGAGGAAGAA AAATGACCAC ACAGAAATCA AAGCTATCCG ACGAAGCCTT 
AGAGCTGTGA GCACGACCGT GGAGGAGGAG AACAAGCTGT ACAGAAGAAC TGAGCGAGCG 
CTTCATCACC TGCTCACCTA CAAACACTTG TCGGCCATTC TGGATGCGTT GAAGCACCTG 
GAGGTCGTTA CCAGACTCTC TCCACTTTGC TGTGAGAATA TGGCTGAAAG CGGAGCGGTA 
TCTACCATCT TTGTTGTGAT CCGAAGCTGT AATCGGAGTG TTCCTTGTAT GGAAGTCGTT 
GGATATGCTG TTCAAGTATT GCTGAATGTG GCAAAGTATG ATAAGACGAT CGCGGCTGTG 
TACGAGGCCG AGAACTGCGT GGACACACTG CTGGAGCTTC TGCAGGTGTA CCGAGAGAAG 
CCTGGAGACC GAGTCGCTGA GAAGAGTGCC AGCATTTTCA CAAGAACTTG CTGTTTGTTA 
GCAGTGCTGT TGAAGACAGA GCAGTGTGCT TTTGATGCAC AAAGTAGATC TAAAGTCACT 
GATCGTATTT ATCGTCTCTA CAAATTTACA GTTCCTAAGC ATAAAGTAAA TACACAGGGA 
TTGTTTGATA AGCAGAAGCA GAATTCCTGT GTAGGCTTTC CTTGTATACC AGAAAGAACT 
ATGAAGACCA GGTTAGTTTC AAGACTTAAG CCACAGTGGG TTTTGAGGCG TGATAATGTG 
GAAGAAATTA CAAACTCCCT GCAAGCTATT CAACTGGTAA TGGATACACT TGGAATTTCT 
TATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006529171.1
CDS	256..5961
Translation	MVMTASICIFLSGNICSTSLDPHTHFLSCQADKDHPEERDKAARVIQSVVLNFLSRRRLQKNVSAALVIQKCWRRVSAQRKLRMLKNEKLAKLQNKSAVLIQAYWRRYSTRKRFLRLKHYSVILQSRIRMKIALTSYKRYLWATVTIQRHWRAYLSRKRDQQIFRKLKSSSLVIQFMFRRWKRRKLQLQTKAAVTLQRAFREWHLRKQIRERSAVVIQSWYRMHRELQKYIYIRSCVIVIQRRVRCFQAQKLYKRRKDAILTLQKHYRARQKGKLAHADYLQKRAATIRLQAAFRGMKARHSYRLQIGAACVLQSYWRMRQERVRFLNLKKMVIKLQAHIRKYQQLQKYKKIKKAAITIQTHFRASISARRVLASYQKTRSSVIVLQSACRGMQARKAFRHALASVIKIQSYYRAYICRKTFQNFKNATIKLQSIVKMKQSRKQYLQIRAAALFIQRWYRSQKLASQKRKEYIQVRESCIKLQSHFRGCLVRKQLRLQCKAAISLQSYFRMRTARQRYLKMCKAALVIQSFYCAYRAQISQRKNFLQVKRAAICLQAAYRGCKVRRQIKQQSTAAVTIQRVFRGHSQRMKYQTMLQSAVKIQRWYRAQKVAYDMRIQFLKTREAVVCLQSAYRGWQVRQQLRRQHEAAVKIQSTFRMAVAQQQYKLLRAAAAVIQQHVRARAAGKRQHLAYIQLRHAALVFQAAWKGKMLRRQIARQHQCAALIQSYYRMHIQRRKWSIMKTAALQIQLCYRAYKVGKEQRHLYLKTKAAVVTLQSAYRGMKVRKRVAECHKAAVTIQSKFRAYRTQKKYTTYRTSAIVIQRWYRNIKITTQQHQEYLNLRRAAVQVQAAYRGIRVRRRIQHMHMAATLIEAMFKMRQSRVRYLKMRTAALIIQVRYRAYYLGKIQHEKYLRTLKAIKTLQAGVRGARVRRTVRKMHFAATLIQSHFRGHRQQTYFHRLRKAATMVQQRYRAVKEGSAEFQRYSRLRRSVLLIQAAFRGLRTRRHLKAMHLAATLIQRRFRTFAMRRKFLSLRKTAIWIQRQYRARLYAKYSRQQLLLEKAVIKIQSSYRGWVVRKRVQKMHRAATVIQATFRMHGAYMRYQHLKRASVVIQVHTAAELQRQKHAAVILQAAVRGMKTRSHLKTMHSSATLIQSQFRAFIVRRRFIALRKAAIFVQRKFRATLYAKHKLHQFLQLRKAAITIQSSYRRLMVQKKLQEMHRAAALIQATFRMHRTYVAFHIWKCASIRIQQCYRTYRTIKLQKEKLIREEQHSAAVLIQSTYRMYRQRCFYQQRRWAAKVIQKTYRANKRRQDLLYVCKEETPLLQMHFQGLNTAKQGRQQHGAAMITQKHFRAFKARRLMEAERGFQAGCRKYKAKKYLSKVEAACRIQAWYRRWRAHKKYLTLLKAVNIIEGYLSAQLARRRFLKMRAAAIIIQRKWRATLSVRGARENLKRHRAACVIQAHFRGYQARQSFLQQRSAVLIIQRHVRAMVAAKQERIKYIKLKKSTVVVQALVRGWLVRKRVSEQKAKTRLFHFTAAAYCHMCALKIQRAYRLHVTLRNAKKHMDSVIFIQRWFRKRLQRKRFIEQYHKILSTRREAHACWLQQDRAASVIQKAVRRFLLCRRQEKITSCATRIQALWRGYSWRKKNDHTEIKAIRRSLRAVSTTVEEENKLYRRTERALHHLLTYKHLSAILDALKHLEVVTRLSPLCCENMAESGAVSTIFVVIRSCNRSVPCMEVVGYAVQVLLNVAKYDKTIAAVYEAENCVDTLLELLQVYREKPGDRVAEKSASIFTRTCCLLAVLLKTEQCAFDAQSRSKVTDRIYRLYKFTVPKHKVNTQGLFDKQKQNSCVGFPCIPERTMKTRLVSRLKPQWVLRRDNVEEITNSLQAIQLVMDTLGISY

Target ORF information:

RefSeq Version	XM_006529108.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus abnormal spindle microtubule assembly (Aspm), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006529108.4

ORF Insert Sequence:

ATGGTGATGA CAGCATCTAT CTGTATCTTC CTAAGTGGGA ACATATGCAG CACCTCTCTA 
GATCCTCATA CGCATTTCCT GAGCTGCCAG GCTGATAAGG ATCATCCTGA GGAAAGAGAC 
AAAGCTGCTA GAGTTATTCA GTCAGTTGTT CTCAATTTTC TAAGTAGACG AAGATTGCAA 
AAAAACGTCA GTGCAGCTCT GGTCATTCAG AAATGCTGGC GAAGAGTCTC AGCGCAAAGG 
AAGTTGCGGA TGCTGAAAAA TGAAAAACTG GCAAAACTTC AAAATAAATC AGCAGTGCTC 
ATTCAGGCTT ATTGGAGAAG ATACTCCACC AGAAAACGGT TTCTGAGGTT GAAGCACTAT 
TCAGTCATCC TTCAGTCTAG GATAAGAATG AAAATTGCTC TTACATCTTA CAAACGATAT 
CTTTGGGCCA CAGTTACAAT TCAGAGGCAT TGGCGTGCCT ATTTAAGTAG AAAACGAGAT 
CAGCAAATAT TTAGGAAGCT GAAATCCTCA TCCCTTGTAA TCCAGTTTAT GTTCAGAAGG 
TGGAAGCGAC GCAAGCTGCA GTTACAAACC AAAGCTGCAG TAACATTGCA GCGAGCTTTT 
AGAGAATGGC ATCTCAGGAA ACAAATACGA GAAAGATCCG CTGTTGTGAT ACAGTCATGG 
TACAGAATGC ACAGGGAATT GCAGAAATAC ATTTATATTA GGTCTTGTGT TATTGTCATC 
CAGAGAAGAG TTCGGTGCTT TCAAGCCCAA AAGTTATATA AGAGAAGGAA AGATGCCATC 
CTGACTCTCC AGAAACATTA CAGGGCACGT CAGAAGGGGA AGCTAGCACA CGCTGACTAT 
TTGCAGAAAC GAGCTGCCAC CATTCGACTG CAGGCTGCAT TCAGGGGGAT GAAGGCTCGC 
CATTCATACA GGTTGCAGAT TGGAGCTGCC TGTGTTCTTC AGTCCTATTG GAGAATGAGA 
CAGGAAAGAG TTAGATTTTT AAACCTTAAA AAGATGGTTA TTAAGTTGCA AGCACACATA 
AGAAAATACC AACAACTACA GAAATATAAG AAGATAAAGA AAGCAGCCAT TACAATTCAG 
ACTCATTTCC GAGCTTCCAT TTCAGCCAGG AGGGTTCTAG CATCTTATCA GAAAACAAGG 
TCTTCAGTCA TTGTTCTACA GTCTGCATGT AGAGGGATGC AAGCCAGAAA AGCATTTCGC 
CATGCTTTAG CATCCGTTAT AAAGATTCAG TCATATTATC GGGCTTATAT ATGTAGGAAG 
ACATTTCAGA ACTTCAAAAA TGCCACAATA AAGCTGCAGT CAATTGTCAA AATGAAACAA 
TCACGGAAGC AGTATTTACA AATAAGAGCA GCTGCACTGT TTATCCAGCG GTGGTACCGT 
TCCCAAAAAC TGGCCTCACA AAAGAGAAAA GAGTACATAC AGGTGCGTGA ATCCTGTATC 
AAACTGCAGT CACACTTTAG AGGATGCCTG GTCAGAAAGC AGTTGAGGTT GCAATGTAAA 
GCTGCCATTT CTCTACAGTC TTATTTCAGA ATGAGAACAG CGAGGCAGCG CTACCTGAAG 
ATGTGCAAGG CGGCTCTTGT CATTCAGAGT TTCTACTGTG CCTACAGAGC ACAAATCAGT 
CAAAGGAAGA ACTTCTTGCA GGTCAAAAGA GCAGCTATTT GTTTGCAGGC AGCTTACAGA 
GGCTGCAAAG TACGCCGGCA AATCAAGCAG CAGTCTACGG CTGCTGTTAC AATTCAGAGG 
GTTTTTAGAG GCCACAGTCA GAGGATGAAA TACCAGACTA TGCTTCAGTC TGCTGTGAAG 
ATTCAGAGAT GGTACAGGGC ACAGAAGGTT GCTTATGACA TGAGAATTCA ATTTCTAAAG 
ACTAGAGAGG CTGTGGTTTG CCTCCAGTCT GCCTACCGTG GCTGGCAGGT TCGGCAACAG 
CTCAGGAGGC AACATGAGGC TGCAGTGAAG ATCCAGTCTA CGTTTAGAAT GGCCGTGGCC 
CAGCAACAGT ATAAACTACT AAGAGCAGCT GCAGCAGTCA TCCAGCAGCA TGTGAGGGCA 
CGGGCTGCAG GGAAGAGGCA GCACTTGGCG TACATCCAGC TCCGTCATGC AGCTCTGGTG 
TTTCAGGCTG CATGGAAGGG AAAGATGCTG AGAAGACAGA TTGCCCGGCA GCATCAATGC 
GCTGCCCTCA TACAGTCATA TTACAGGATG CACATTCAGC GCAGGAAGTG GAGCATCATG 
AAAACCGCTG CCCTTCAGAT TCAGCTGTGC TATAGGGCTT ACAAAGTTGG AAAAGAACAG 
CGCCACTTAT ACTTGAAAAC AAAAGCAGCT GTAGTAACTT TACAGTCAGC TTACCGTGGT 
ATGAAAGTGA GAAAGCGGGT AGCTGAATGC CACAAAGCAG CGGTCACTAT ACAGTCTAAA 
TTTAGAGCCT ACAGAACACA AAAGAAGTAC ACGACCTATA GAACTTCAGC TATTGTAATT 
CAGAGGTGGT ACCGGAATAT TAAAATTACA ACCCAGCAAC ATCAGGAATA CCTTAACTTA 
AGGAGGGCAG CAGTTCAAGT CCAGGCTGCG TATAGAGGCA TTAGAGTGAG GAGACGCATT 
CAACACATGC ACATGGCGGC CACACTTATT GAAGCCATGT TTAAGATGCG TCAGTCAAGA 
GTAAGGTACC TCAAGATGAG AACAGCAGCT CTTATAATTC AGGTGAGATA CAGAGCGTAT 
TATCTAGGTA AGATTCAGCA TGAGAAGTAC CTGAGGACTT TGAAGGCTAT TAAAACCCTT 
CAAGCTGGAG TCAGAGGAGC AAGAGTGAGG CGGACTGTAA GAAAGATGCA TTTCGCAGCA 
ACACTCATTC AGTCACATTT TAGGGGACAC AGGCAGCAAA CTTACTTTCA TCGGTTGAGG 
AAGGCGGCAA CCATGGTGCA GCAGAGATAC CGAGCAGTGA AAGAAGGAAG CGCTGAGTTT 
CAGAGGTACA GCAGACTAAG GCGTTCTGTA CTCCTCATCC AGGCTGCCTT CAGGGGACTG 
AGAACTAGGA GACACTTAAA AGCTATGCAC CTGGCTGCTA CTCTCATTCA GAGGAGATTC 
AGGACTTTCG CGATGAGAAG GAAGTTCCTT TCCCTCAGGA AAACTGCCAT TTGGATTCAG 
AGACAGTACC GTGCAAGACT TTATGCCAAG TATTCCCGAC AACAGTTACT GTTGGAAAAG 
GCAGTTATAA AGATCCAGTC ATCATACAGA GGATGGGTGG TAAGGAAAAG GGTGCAAAAG 
ATGCACAGGG CGGCTACTGT GATTCAGGCC ACTTTCCGGA TGCACGGAGC CTATATGAGG 
TACCAGCATT TGAAGCGGGC CTCAGTTGTG ATCCAAGTAC ACACAGCTGC AGAACTGCAG 
AGGCAAAAGC ATGCTGCTGT AATCCTTCAG GCTGCCGTTA GGGGGATGAA AACCAGAAGT 
CATCTGAAGA CTATGCATTC TTCTGCAACC CTTATTCAGA GTCAATTTAG AGCTTTTATA 
GTAAGGAGAC GATTCATTGC TCTCAGAAAA GCTGCTATAT TTGTGCAGAG GAAATTCCGA 
GCCACCCTTT ATGCCAAGCA TAAATTGCAC CAATTCTTGC AGTTACGAAA GGCAGCCATT 
ACCATACAGT CCTCTTATCG AAGACTGATG GTACAAAAGA AGTTACAAGA AATGCACAGG 
GCTGCAGCTC TCATCCAGGC TACTTTTAGA ATGCACAGAA CTTACGTTGC ATTTCACATT 
TGGAAATGTG CCTCGATTAG AATTCAGCAG TGTTATCGAA CATACAGAAC TATAAAATTA 
CAAAAAGAAA AACTTATCAG AGAAGAACAA CACAGTGCTG CTGTCCTAAT ACAAAGCACG 
TACAGGATGT ACAGGCAGCG TTGCTTCTAT CAACAGCGTC GGTGGGCAGC AAAGGTAATA 
CAAAAAACAT ACAGAGCAAA TAAAAGACGG CAAGATCTGC TCTATGTGTG TAAGGAAGAA 
ACACCTCTTC TTCAGATGCA TTTTCAGGGT TTAAACACTG CAAAACAAGG TCGGCAGCAG 
CATGGGGCCG CTATGATTAC ACAGAAGCAT TTTAGAGCCT TTAAAGCAAG GAGGCTGATG 
GAGGCCGAGA GAGGCTTCCA GGCTGGATGT AGAAAATATA AAGCTAAGAA ATACTTATCC 
AAAGTGGAAG CTGCCTGCAG GATTCAAGCT TGGTATAGGC GTTGGAGAGC GCACAAGAAA 
TATCTAACTT TATTGAAAGC TGTTAACATT ATTGAAGGTT ACCTCTCTGC ACAGCTGGCG 
AGAAGGAGGT TCTTGAAGAT GAGGGCGGCA GCTATTATAA TTCAAAGGAA ATGGAGAGCT 
ACACTTTCTG TAAGAGGAGC TCGTGAGAAT TTAAAAAGAC ATCGAGCTGC TTGTGTGATT 
CAAGCCCACT TTAGGGGCTA TCAGGCAAGG CAGTCCTTCC TGCAGCAGAG ATCTGCTGTT 
TTAATCATAC AGAGACATGT ACGAGCCATG GTGGCTGCGA AGCAGGAAAG GATAAAGTAT 
ATCAAATTGA AAAAGTCTAC GGTTGTTGTA CAAGCCTTGG TGCGTGGCTG GTTAGTAAGG 
AAAAGGGTTT CAGAGCAGAA AGCCAAAACT CGGCTTTTCC ACTTTACAGC AGCTGCCTAC 
TGTCACATGT GTGCTCTTAA AATCCAACGA GCTTATCGGC TCCACGTGAC CTTGAGGAAC 
GCTAAGAAGC ACATGGATTC AGTCATCTTC ATTCAGAGAT GGTTTCGAAA AAGATTACAA 
CGAAAGAGGT TCATTGAACA GTACCACAAG ATCCTAAGCA CTCGACGTGA AGCTCATGCA 
TGTTGGCTCC AGCAGGACAG GGCTGCATCC GTGATACAGA AGGCGGTACG CCGCTTTCTC 
CTCTGCAGAA GACAGGAAAA GATCACTAGC TGCGCCACTA GAATTCAGGC ATTATGGAGA 
GGCTATTCTT GGAGGAAGAA AAATGACCAC ACAGAAATCA AAGCTATCCG ACGAAGCCTT 
AGAGCTGTGA GCACGACCGT GGAGGAGGAG AACAAGCTGT ACAGAAGAAC TGAGCGAGCG 
CTTCATCACC TGCTCACCTA CAAACACTTG TCGGCCATTC TGGATGCGTT GAAGCACCTG 
GAGGTCGTTA CCAGACTCTC TCCACTTTGC TGTGAGAATA TGGCTGAAAG CGGAGCGGTA 
TCTACCATCT TTGTTGTGAT CCGAAGCTGT AATCGGAGTG TTCCTTGTAT GGAAGTCGTT 
GGATATGCTG TTCAAGTATT GCTGAATGTG GCAAAGTATG ATAAGACGAT CGCGGCTGTG 
TACGAGGCCG AGAACTGCGT GGACACACTG CTGGAGCTTC TGCAGGTGTA CCGAGAGAAG 
CCTGGAGACC GAGTCGCTGA GAAGAGTGCC AGCATTTTCA CAAGAACTTG CTGTTTGTTA 
GCAGTGCTGT TGAAGACAGA GCAGTGTGCT TTTGATGCAC AAAGTAGATC TAAAGTCACT 
GATCGTATTT ATCGTCTCTA CAAATTTACA GTTCCTAAGC ATAAAGTAAA TACACAGGGA 
TTGTTTGATA AGCAGAAGCA GAATTCCTGT GTAGGCTTTC CTTGTATACC AGAAAGAACT 
ATGAAGACCA GGTTAGTTTC AAGACTTAAG CCACAGTGGG TTTTGAGGCG TGATAATGTG 
GAAGAAATTA CAAACTCCCT GCAAGCTATT CAACTGGTAA TGGATACACT TGGAATTTCT 
TATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Aspm ( XM_006529108.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006529171.1 Mus musculus asp (abnormal spindle)-like, microcephaly associated (Drosophila) (Aspm), transcript variant X1, mRNA.

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CloneID	OMu27884
Clone ID Related Accession (Same CDS sequence)	XM_006529108.3 , XM_006529108.4
Accession Version	XM_006529108.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5706bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	abnormal spindle-like microcephaly-associated protein homolog isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006529108.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGATGA CAGCATCTAT CTGTATCTTC CTAAGTGGGA ACATATGCAG CACCTCTCTA 
GATCCTCATA CGCATTTCCT GAGCTGCCAG GCTGATAAGG ATCATCCTGA GGAAAGAGAC 
AAAGCTGCTA GAGTTATTCA GTCAGTTGTT CTCAATTTTC TAAGTAGACG AAGATTGCAA 
AAAAACGTCA GTGCAGCTCT GGTCATTCAG AAATGCTGGC GAAGAGTCTC AGCGCAAAGG 
AAGTTGCGGA TGCTGAAAAA TGAAAAACTG GCAAAACTTC AAAATAAATC AGCAGTGCTC 
ATTCAGGCTT ATTGGAGAAG ATACTCCACC AGAAAACGGT TTCTGAGGTT GAAGCACTAT 
TCAGTCATCC TTCAGTCTAG GATAAGAATG AAAATTGCTC TTACATCTTA CAAACGATAT 
CTTTGGGCCA CAGTTACAAT TCAGAGGCAT TGGCGTGCCT ATTTAAGTAG AAAACGAGAT 
CAGCAAATAT TTAGGAAGCT GAAATCCTCA TCCCTTGTAA TCCAGTTTAT GTTCAGAAGG 
TGGAAGCGAC GCAAGCTGCA GTTACAAACC AAAGCTGCAG TAACATTGCA GCGAGCTTTT 
AGAGAATGGC ATCTCAGGAA ACAAATACGA GAAAGATCCG CTGTTGTGAT ACAGTCATGG 
TACAGAATGC ACAGGGAATT GCAGAAATAC ATTTATATTA GGTCTTGTGT TATTGTCATC 
CAGAGAAGAG TTCGGTGCTT TCAAGCCCAA AAGTTATATA AGAGAAGGAA AGATGCCATC 
CTGACTCTCC AGAAACATTA CAGGGCACGT CAGAAGGGGA AGCTAGCACA CGCTGACTAT 
TTGCAGAAAC GAGCTGCCAC CATTCGACTG CAGGCTGCAT TCAGGGGGAT GAAGGCTCGC 
CATTCATACA GGTTGCAGAT TGGAGCTGCC TGTGTTCTTC AGTCCTATTG GAGAATGAGA 
CAGGAAAGAG TTAGATTTTT AAACCTTAAA AAGATGGTTA TTAAGTTGCA AGCACACATA 
AGAAAATACC AACAACTACA GAAATATAAG AAGATAAAGA AAGCAGCCAT TACAATTCAG 
ACTCATTTCC GAGCTTCCAT TTCAGCCAGG AGGGTTCTAG CATCTTATCA GAAAACAAGG 
TCTTCAGTCA TTGTTCTACA GTCTGCATGT AGAGGGATGC AAGCCAGAAA AGCATTTCGC 
CATGCTTTAG CATCCGTTAT AAAGATTCAG TCATATTATC GGGCTTATAT ATGTAGGAAG 
ACATTTCAGA ACTTCAAAAA TGCCACAATA AAGCTGCAGT CAATTGTCAA AATGAAACAA 
TCACGGAAGC AGTATTTACA AATAAGAGCA GCTGCACTGT TTATCCAGCG GTGGTACCGT 
TCCCAAAAAC TGGCCTCACA AAAGAGAAAA GAGTACATAC AGGTGCGTGA ATCCTGTATC 
AAACTGCAGT CACACTTTAG AGGATGCCTG GTCAGAAAGC AGTTGAGGTT GCAATGTAAA 
GCTGCCATTT CTCTACAGTC TTATTTCAGA ATGAGAACAG CGAGGCAGCG CTACCTGAAG 
ATGTGCAAGG CGGCTCTTGT CATTCAGAGT TTCTACTGTG CCTACAGAGC ACAAATCAGT 
CAAAGGAAGA ACTTCTTGCA GGTCAAAAGA GCAGCTATTT GTTTGCAGGC AGCTTACAGA 
GGCTGCAAAG TACGCCGGCA AATCAAGCAG CAGTCTACGG CTGCTGTTAC AATTCAGAGG 
GTTTTTAGAG GCCACAGTCA GAGGATGAAA TACCAGACTA TGCTTCAGTC TGCTGTGAAG 
ATTCAGAGAT GGTACAGGGC ACAGAAGGTT GCTTATGACA TGAGAATTCA ATTTCTAAAG 
ACTAGAGAGG CTGTGGTTTG CCTCCAGTCT GCCTACCGTG GCTGGCAGGT TCGGCAACAG 
CTCAGGAGGC AACATGAGGC TGCAGTGAAG ATCCAGTCTA CGTTTAGAAT GGCCGTGGCC 
CAGCAACAGT ATAAACTACT AAGAGCAGCT GCAGCAGTCA TCCAGCAGCA TGTGAGGGCA 
CGGGCTGCAG GGAAGAGGCA GCACTTGGCG TACATCCAGC TCCGTCATGC AGCTCTGGTG 
TTTCAGGCTG CATGGAAGGG AAAGATGCTG AGAAGACAGA TTGCCCGGCA GCATCAATGC 
GCTGCCCTCA TACAGTCATA TTACAGGATG CACATTCAGC GCAGGAAGTG GAGCATCATG 
AAAACCGCTG CCCTTCAGAT TCAGCTGTGC TATAGGGCTT ACAAAGTTGG AAAAGAACAG 
CGCCACTTAT ACTTGAAAAC AAAAGCAGCT GTAGTAACTT TACAGTCAGC TTACCGTGGT 
ATGAAAGTGA GAAAGCGGGT AGCTGAATGC CACAAAGCAG CGGTCACTAT ACAGTCTAAA 
TTTAGAGCCT ACAGAACACA AAAGAAGTAC ACGACCTATA GAACTTCAGC TATTGTAATT 
CAGAGGTGGT ACCGGAATAT TAAAATTACA ACCCAGCAAC ATCAGGAATA CCTTAACTTA 
AGGAGGGCAG CAGTTCAAGT CCAGGCTGCG TATAGAGGCA TTAGAGTGAG GAGACGCATT 
CAACACATGC ACATGGCGGC CACACTTATT GAAGCCATGT TTAAGATGCG TCAGTCAAGA 
GTAAGGTACC TCAAGATGAG AACAGCAGCT CTTATAATTC AGGTGAGATA CAGAGCGTAT 
TATCTAGGTA AGATTCAGCA TGAGAAGTAC CTGAGGACTT TGAAGGCTAT TAAAACCCTT 
CAAGCTGGAG TCAGAGGAGC AAGAGTGAGG CGGACTGTAA GAAAGATGCA TTTCGCAGCA 
ACACTCATTC AGTCACATTT TAGGGGACAC AGGCAGCAAA CTTACTTTCA TCGGTTGAGG 
AAGGCGGCAA CCATGGTGCA GCAGAGATAC CGAGCAGTGA AAGAAGGAAG CGCTGAGTTT 
CAGAGGTACA GCAGACTAAG GCGTTCTGTA CTCCTCATCC AGGCTGCCTT CAGGGGACTG 
AGAACTAGGA GACACTTAAA AGCTATGCAC CTGGCTGCTA CTCTCATTCA GAGGAGATTC 
AGGACTTTCG CGATGAGAAG GAAGTTCCTT TCCCTCAGGA AAACTGCCAT TTGGATTCAG 
AGACAGTACC GTGCAAGACT TTATGCCAAG TATTCCCGAC AACAGTTACT GTTGGAAAAG 
GCAGTTATAA AGATCCAGTC ATCATACAGA GGATGGGTGG TAAGGAAAAG GGTGCAAAAG 
ATGCACAGGG CGGCTACTGT GATTCAGGCC ACTTTCCGGA TGCACGGAGC CTATATGAGG 
TACCAGCATT TGAAGCGGGC CTCAGTTGTG ATCCAAGTAC ACACAGCTGC AGAACTGCAG 
AGGCAAAAGC ATGCTGCTGT AATCCTTCAG GCTGCCGTTA GGGGGATGAA AACCAGAAGT 
CATCTGAAGA CTATGCATTC TTCTGCAACC CTTATTCAGA GTCAATTTAG AGCTTTTATA 
GTAAGGAGAC GATTCATTGC TCTCAGAAAA GCTGCTATAT TTGTGCAGAG GAAATTCCGA 
GCCACCCTTT ATGCCAAGCA TAAATTGCAC CAATTCTTGC AGTTACGAAA GGCAGCCATT 
ACCATACAGT CCTCTTATCG AAGACTGATG GTACAAAAGA AGTTACAAGA AATGCACAGG 
GCTGCAGCTC TCATCCAGGC TACTTTTAGA ATGCACAGAA CTTACGTTGC ATTTCACATT 
TGGAAATGTG CCTCGATTAG AATTCAGCAG TGTTATCGAA CATACAGAAC TATAAAATTA 
CAAAAAGAAA AACTTATCAG AGAAGAACAA CACAGTGCTG CTGTCCTAAT ACAAAGCACG 
TACAGGATGT ACAGGCAGCG TTGCTTCTAT CAACAGCGTC GGTGGGCAGC AAAGGTAATA 
CAAAAAACAT ACAGAGCAAA TAAAAGACGG CAAGATCTGC TCTATGTGTG TAAGGAAGAA 
ACACCTCTTC TTCAGATGCA TTTTCAGGGT TTAAACACTG CAAAACAAGG TCGGCAGCAG 
CATGGGGCCG CTATGATTAC ACAGAAGCAT TTTAGAGCCT TTAAAGCAAG GAGGCTGATG 
GAGGCCGAGA GAGGCTTCCA GGCTGGATGT AGAAAATATA AAGCTAAGAA ATACTTATCC 
AAAGTGGAAG CTGCCTGCAG GATTCAAGCT TGGTATAGGC GTTGGAGAGC GCACAAGAAA 
TATCTAACTT TATTGAAAGC TGTTAACATT ATTGAAGGTT ACCTCTCTGC ACAGCTGGCG 
AGAAGGAGGT TCTTGAAGAT GAGGGCGGCA GCTATTATAA TTCAAAGGAA ATGGAGAGCT 
ACACTTTCTG TAAGAGGAGC TCGTGAGAAT TTAAAAAGAC ATCGAGCTGC TTGTGTGATT 
CAAGCCCACT TTAGGGGCTA TCAGGCAAGG CAGTCCTTCC TGCAGCAGAG ATCTGCTGTT 
TTAATCATAC AGAGACATGT ACGAGCCATG GTGGCTGCGA AGCAGGAAAG GATAAAGTAT 
ATCAAATTGA AAAAGTCTAC GGTTGTTGTA CAAGCCTTGG TGCGTGGCTG GTTAGTAAGG 
AAAAGGGTTT CAGAGCAGAA AGCCAAAACT CGGCTTTTCC ACTTTACAGC AGCTGCCTAC 
TGTCACATGT GTGCTCTTAA AATCCAACGA GCTTATCGGC TCCACGTGAC CTTGAGGAAC 
GCTAAGAAGC ACATGGATTC AGTCATCTTC ATTCAGAGAT GGTTTCGAAA AAGATTACAA 
CGAAAGAGGT TCATTGAACA GTACCACAAG ATCCTAAGCA CTCGACGTGA AGCTCATGCA 
TGTTGGCTCC AGCAGGACAG GGCTGCATCC GTGATACAGA AGGCGGTACG CCGCTTTCTC 
CTCTGCAGAA GACAGGAAAA GATCACTAGC TGCGCCACTA GAATTCAGGC ATTATGGAGA 
GGCTATTCTT GGAGGAAGAA AAATGACCAC ACAGAAATCA AAGCTATCCG ACGAAGCCTT 
AGAGCTGTGA GCACGACCGT GGAGGAGGAG AACAAGCTGT ACAGAAGAAC TGAGCGAGCG 
CTTCATCACC TGCTCACCTA CAAACACTTG TCGGCCATTC TGGATGCGTT GAAGCACCTG 
GAGGTCGTTA CCAGACTCTC TCCACTTTGC TGTGAGAATA TGGCTGAAAG CGGAGCGGTA 
TCTACCATCT TTGTTGTGAT CCGAAGCTGT AATCGGAGTG TTCCTTGTAT GGAAGTCGTT 
GGATATGCTG TTCAAGTATT GCTGAATGTG GCAAAGTATG ATAAGACGAT CGCGGCTGTG 
TACGAGGCCG AGAACTGCGT GGACACACTG CTGGAGCTTC TGCAGGTGTA CCGAGAGAAG 
CCTGGAGACC GAGTCGCTGA GAAGAGTGCC AGCATTTTCA CAAGAACTTG CTGTTTGTTA 
GCAGTGCTGT TGAAGACAGA GCAGTGTGCT TTTGATGCAC AAAGTAGATC TAAAGTCACT 
GATCGTATTT ATCGTCTCTA CAAATTTACA GTTCCTAAGC ATAAAGTAAA TACACAGGGA 
TTGTTTGATA AGCAGAAGCA GAATTCCTGT GTAGGCTTTC CTTGTATACC AGAAAGAACT 
ATGAAGACCA GGTTAGTTTC AAGACTTAAG CCACAGTGGG TTTTGAGGCG TGATAATGTG 
GAAGAAATTA CAAACTCCCT GCAAGCTATT CAACTGGTAA TGGATACACT TGGAATTTCT 
TATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006529171.1
CDS	267..5972
Translation	MVMTASICIFLSGNICSTSLDPHTHFLSCQADKDHPEERDKAARVIQSVVLNFLSRRRLQKNVSAALVIQKCWRRVSAQRKLRMLKNEKLAKLQNKSAVLIQAYWRRYSTRKRFLRLKHYSVILQSRIRMKIALTSYKRYLWATVTIQRHWRAYLSRKRDQQIFRKLKSSSLVIQFMFRRWKRRKLQLQTKAAVTLQRAFREWHLRKQIRERSAVVIQSWYRMHRELQKYIYIRSCVIVIQRRVRCFQAQKLYKRRKDAILTLQKHYRARQKGKLAHADYLQKRAATIRLQAAFRGMKARHSYRLQIGAACVLQSYWRMRQERVRFLNLKKMVIKLQAHIRKYQQLQKYKKIKKAAITIQTHFRASISARRVLASYQKTRSSVIVLQSACRGMQARKAFRHALASVIKIQSYYRAYICRKTFQNFKNATIKLQSIVKMKQSRKQYLQIRAAALFIQRWYRSQKLASQKRKEYIQVRESCIKLQSHFRGCLVRKQLRLQCKAAISLQSYFRMRTARQRYLKMCKAALVIQSFYCAYRAQISQRKNFLQVKRAAICLQAAYRGCKVRRQIKQQSTAAVTIQRVFRGHSQRMKYQTMLQSAVKIQRWYRAQKVAYDMRIQFLKTREAVVCLQSAYRGWQVRQQLRRQHEAAVKIQSTFRMAVAQQQYKLLRAAAAVIQQHVRARAAGKRQHLAYIQLRHAALVFQAAWKGKMLRRQIARQHQCAALIQSYYRMHIQRRKWSIMKTAALQIQLCYRAYKVGKEQRHLYLKTKAAVVTLQSAYRGMKVRKRVAECHKAAVTIQSKFRAYRTQKKYTTYRTSAIVIQRWYRNIKITTQQHQEYLNLRRAAVQVQAAYRGIRVRRRIQHMHMAATLIEAMFKMRQSRVRYLKMRTAALIIQVRYRAYYLGKIQHEKYLRTLKAIKTLQAGVRGARVRRTVRKMHFAATLIQSHFRGHRQQTYFHRLRKAATMVQQRYRAVKEGSAEFQRYSRLRRSVLLIQAAFRGLRTRRHLKAMHLAATLIQRRFRTFAMRRKFLSLRKTAIWIQRQYRARLYAKYSRQQLLLEKAVIKIQSSYRGWVVRKRVQKMHRAATVIQATFRMHGAYMRYQHLKRASVVIQVHTAAELQRQKHAAVILQAAVRGMKTRSHLKTMHSSATLIQSQFRAFIVRRRFIALRKAAIFVQRKFRATLYAKHKLHQFLQLRKAAITIQSSYRRLMVQKKLQEMHRAAALIQATFRMHRTYVAFHIWKCASIRIQQCYRTYRTIKLQKEKLIREEQHSAAVLIQSTYRMYRQRCFYQQRRWAAKVIQKTYRANKRRQDLLYVCKEETPLLQMHFQGLNTAKQGRQQHGAAMITQKHFRAFKARRLMEAERGFQAGCRKYKAKKYLSKVEAACRIQAWYRRWRAHKKYLTLLKAVNIIEGYLSAQLARRRFLKMRAAAIIIQRKWRATLSVRGARENLKRHRAACVIQAHFRGYQARQSFLQQRSAVLIIQRHVRAMVAAKQERIKYIKLKKSTVVVQALVRGWLVRKRVSEQKAKTRLFHFTAAAYCHMCALKIQRAYRLHVTLRNAKKHMDSVIFIQRWFRKRLQRKRFIEQYHKILSTRREAHACWLQQDRAASVIQKAVRRFLLCRRQEKITSCATRIQALWRGYSWRKKNDHTEIKAIRRSLRAVSTTVEEENKLYRRTERALHHLLTYKHLSAILDALKHLEVVTRLSPLCCENMAESGAVSTIFVVIRSCNRSVPCMEVVGYAVQVLLNVAKYDKTIAAVYEAENCVDTLLELLQVYREKPGDRVAEKSASIFTRTCCLLAVLLKTEQCAFDAQSRSKVTDRIYRLYKFTVPKHKVNTQGLFDKQKQNSCVGFPCIPERTMKTRLVSRLKPQWVLRRDNVEEITNSLQAIQLVMDTLGISY

Target ORF information:

RefSeq Version	XM_006529108.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus asp (abnormal spindle)-like, microcephaly associated (Drosophila) (Aspm), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006529108.3

ORF Insert Sequence:

ATGGTGATGA CAGCATCTAT CTGTATCTTC CTAAGTGGGA ACATATGCAG CACCTCTCTA 
GATCCTCATA CGCATTTCCT GAGCTGCCAG GCTGATAAGG ATCATCCTGA GGAAAGAGAC 
AAAGCTGCTA GAGTTATTCA GTCAGTTGTT CTCAATTTTC TAAGTAGACG AAGATTGCAA 
AAAAACGTCA GTGCAGCTCT GGTCATTCAG AAATGCTGGC GAAGAGTCTC AGCGCAAAGG 
AAGTTGCGGA TGCTGAAAAA TGAAAAACTG GCAAAACTTC AAAATAAATC AGCAGTGCTC 
ATTCAGGCTT ATTGGAGAAG ATACTCCACC AGAAAACGGT TTCTGAGGTT GAAGCACTAT 
TCAGTCATCC TTCAGTCTAG GATAAGAATG AAAATTGCTC TTACATCTTA CAAACGATAT 
CTTTGGGCCA CAGTTACAAT TCAGAGGCAT TGGCGTGCCT ATTTAAGTAG AAAACGAGAT 
CAGCAAATAT TTAGGAAGCT GAAATCCTCA TCCCTTGTAA TCCAGTTTAT GTTCAGAAGG 
TGGAAGCGAC GCAAGCTGCA GTTACAAACC AAAGCTGCAG TAACATTGCA GCGAGCTTTT 
AGAGAATGGC ATCTCAGGAA ACAAATACGA GAAAGATCCG CTGTTGTGAT ACAGTCATGG 
TACAGAATGC ACAGGGAATT GCAGAAATAC ATTTATATTA GGTCTTGTGT TATTGTCATC 
CAGAGAAGAG TTCGGTGCTT TCAAGCCCAA AAGTTATATA AGAGAAGGAA AGATGCCATC 
CTGACTCTCC AGAAACATTA CAGGGCACGT CAGAAGGGGA AGCTAGCACA CGCTGACTAT 
TTGCAGAAAC GAGCTGCCAC CATTCGACTG CAGGCTGCAT TCAGGGGGAT GAAGGCTCGC 
CATTCATACA GGTTGCAGAT TGGAGCTGCC TGTGTTCTTC AGTCCTATTG GAGAATGAGA 
CAGGAAAGAG TTAGATTTTT AAACCTTAAA AAGATGGTTA TTAAGTTGCA AGCACACATA 
AGAAAATACC AACAACTACA GAAATATAAG AAGATAAAGA AAGCAGCCAT TACAATTCAG 
ACTCATTTCC GAGCTTCCAT TTCAGCCAGG AGGGTTCTAG CATCTTATCA GAAAACAAGG 
TCTTCAGTCA TTGTTCTACA GTCTGCATGT AGAGGGATGC AAGCCAGAAA AGCATTTCGC 
CATGCTTTAG CATCCGTTAT AAAGATTCAG TCATATTATC GGGCTTATAT ATGTAGGAAG 
ACATTTCAGA ACTTCAAAAA TGCCACAATA AAGCTGCAGT CAATTGTCAA AATGAAACAA 
TCACGGAAGC AGTATTTACA AATAAGAGCA GCTGCACTGT TTATCCAGCG GTGGTACCGT 
TCCCAAAAAC TGGCCTCACA AAAGAGAAAA GAGTACATAC AGGTGCGTGA ATCCTGTATC 
AAACTGCAGT CACACTTTAG AGGATGCCTG GTCAGAAAGC AGTTGAGGTT GCAATGTAAA 
GCTGCCATTT CTCTACAGTC TTATTTCAGA ATGAGAACAG CGAGGCAGCG CTACCTGAAG 
ATGTGCAAGG CGGCTCTTGT CATTCAGAGT TTCTACTGTG CCTACAGAGC ACAAATCAGT 
CAAAGGAAGA ACTTCTTGCA GGTCAAAAGA GCAGCTATTT GTTTGCAGGC AGCTTACAGA 
GGCTGCAAAG TACGCCGGCA AATCAAGCAG CAGTCTACGG CTGCTGTTAC AATTCAGAGG 
GTTTTTAGAG GCCACAGTCA GAGGATGAAA TACCAGACTA TGCTTCAGTC TGCTGTGAAG 
ATTCAGAGAT GGTACAGGGC ACAGAAGGTT GCTTATGACA TGAGAATTCA ATTTCTAAAG 
ACTAGAGAGG CTGTGGTTTG CCTCCAGTCT GCCTACCGTG GCTGGCAGGT TCGGCAACAG 
CTCAGGAGGC AACATGAGGC TGCAGTGAAG ATCCAGTCTA CGTTTAGAAT GGCCGTGGCC 
CAGCAACAGT ATAAACTACT AAGAGCAGCT GCAGCAGTCA TCCAGCAGCA TGTGAGGGCA 
CGGGCTGCAG GGAAGAGGCA GCACTTGGCG TACATCCAGC TCCGTCATGC AGCTCTGGTG 
TTTCAGGCTG CATGGAAGGG AAAGATGCTG AGAAGACAGA TTGCCCGGCA GCATCAATGC 
GCTGCCCTCA TACAGTCATA TTACAGGATG CACATTCAGC GCAGGAAGTG GAGCATCATG 
AAAACCGCTG CCCTTCAGAT TCAGCTGTGC TATAGGGCTT ACAAAGTTGG AAAAGAACAG 
CGCCACTTAT ACTTGAAAAC AAAAGCAGCT GTAGTAACTT TACAGTCAGC TTACCGTGGT 
ATGAAAGTGA GAAAGCGGGT AGCTGAATGC CACAAAGCAG CGGTCACTAT ACAGTCTAAA 
TTTAGAGCCT ACAGAACACA AAAGAAGTAC ACGACCTATA GAACTTCAGC TATTGTAATT 
CAGAGGTGGT ACCGGAATAT TAAAATTACA ACCCAGCAAC ATCAGGAATA CCTTAACTTA 
AGGAGGGCAG CAGTTCAAGT CCAGGCTGCG TATAGAGGCA TTAGAGTGAG GAGACGCATT 
CAACACATGC ACATGGCGGC CACACTTATT GAAGCCATGT TTAAGATGCG TCAGTCAAGA 
GTAAGGTACC TCAAGATGAG AACAGCAGCT CTTATAATTC AGGTGAGATA CAGAGCGTAT 
TATCTAGGTA AGATTCAGCA TGAGAAGTAC CTGAGGACTT TGAAGGCTAT TAAAACCCTT 
CAAGCTGGAG TCAGAGGAGC AAGAGTGAGG CGGACTGTAA GAAAGATGCA TTTCGCAGCA 
ACACTCATTC AGTCACATTT TAGGGGACAC AGGCAGCAAA CTTACTTTCA TCGGTTGAGG 
AAGGCGGCAA CCATGGTGCA GCAGAGATAC CGAGCAGTGA AAGAAGGAAG CGCTGAGTTT 
CAGAGGTACA GCAGACTAAG GCGTTCTGTA CTCCTCATCC AGGCTGCCTT CAGGGGACTG 
AGAACTAGGA GACACTTAAA AGCTATGCAC CTGGCTGCTA CTCTCATTCA GAGGAGATTC 
AGGACTTTCG CGATGAGAAG GAAGTTCCTT TCCCTCAGGA AAACTGCCAT TTGGATTCAG 
AGACAGTACC GTGCAAGACT TTATGCCAAG TATTCCCGAC AACAGTTACT GTTGGAAAAG 
GCAGTTATAA AGATCCAGTC ATCATACAGA GGATGGGTGG TAAGGAAAAG GGTGCAAAAG 
ATGCACAGGG CGGCTACTGT GATTCAGGCC ACTTTCCGGA TGCACGGAGC CTATATGAGG 
TACCAGCATT TGAAGCGGGC CTCAGTTGTG ATCCAAGTAC ACACAGCTGC AGAACTGCAG 
AGGCAAAAGC ATGCTGCTGT AATCCTTCAG GCTGCCGTTA GGGGGATGAA AACCAGAAGT 
CATCTGAAGA CTATGCATTC TTCTGCAACC CTTATTCAGA GTCAATTTAG AGCTTTTATA 
GTAAGGAGAC GATTCATTGC TCTCAGAAAA GCTGCTATAT TTGTGCAGAG GAAATTCCGA 
GCCACCCTTT ATGCCAAGCA TAAATTGCAC CAATTCTTGC AGTTACGAAA GGCAGCCATT 
ACCATACAGT CCTCTTATCG AAGACTGATG GTACAAAAGA AGTTACAAGA AATGCACAGG 
GCTGCAGCTC TCATCCAGGC TACTTTTAGA ATGCACAGAA CTTACGTTGC ATTTCACATT 
TGGAAATGTG CCTCGATTAG AATTCAGCAG TGTTATCGAA CATACAGAAC TATAAAATTA 
CAAAAAGAAA AACTTATCAG AGAAGAACAA CACAGTGCTG CTGTCCTAAT ACAAAGCACG 
TACAGGATGT ACAGGCAGCG TTGCTTCTAT CAACAGCGTC GGTGGGCAGC AAAGGTAATA 
CAAAAAACAT ACAGAGCAAA TAAAAGACGG CAAGATCTGC TCTATGTGTG TAAGGAAGAA 
ACACCTCTTC TTCAGATGCA TTTTCAGGGT TTAAACACTG CAAAACAAGG TCGGCAGCAG 
CATGGGGCCG CTATGATTAC ACAGAAGCAT TTTAGAGCCT TTAAAGCAAG GAGGCTGATG 
GAGGCCGAGA GAGGCTTCCA GGCTGGATGT AGAAAATATA AAGCTAAGAA ATACTTATCC 
AAAGTGGAAG CTGCCTGCAG GATTCAAGCT TGGTATAGGC GTTGGAGAGC GCACAAGAAA 
TATCTAACTT TATTGAAAGC TGTTAACATT ATTGAAGGTT ACCTCTCTGC ACAGCTGGCG 
AGAAGGAGGT TCTTGAAGAT GAGGGCGGCA GCTATTATAA TTCAAAGGAA ATGGAGAGCT 
ACACTTTCTG TAAGAGGAGC TCGTGAGAAT TTAAAAAGAC ATCGAGCTGC TTGTGTGATT 
CAAGCCCACT TTAGGGGCTA TCAGGCAAGG CAGTCCTTCC TGCAGCAGAG ATCTGCTGTT 
TTAATCATAC AGAGACATGT ACGAGCCATG GTGGCTGCGA AGCAGGAAAG GATAAAGTAT 
ATCAAATTGA AAAAGTCTAC GGTTGTTGTA CAAGCCTTGG TGCGTGGCTG GTTAGTAAGG 
AAAAGGGTTT CAGAGCAGAA AGCCAAAACT CGGCTTTTCC ACTTTACAGC AGCTGCCTAC 
TGTCACATGT GTGCTCTTAA AATCCAACGA GCTTATCGGC TCCACGTGAC CTTGAGGAAC 
GCTAAGAAGC ACATGGATTC AGTCATCTTC ATTCAGAGAT GGTTTCGAAA AAGATTACAA 
CGAAAGAGGT TCATTGAACA GTACCACAAG ATCCTAAGCA CTCGACGTGA AGCTCATGCA 
TGTTGGCTCC AGCAGGACAG GGCTGCATCC GTGATACAGA AGGCGGTACG CCGCTTTCTC 
CTCTGCAGAA GACAGGAAAA GATCACTAGC TGCGCCACTA GAATTCAGGC ATTATGGAGA 
GGCTATTCTT GGAGGAAGAA AAATGACCAC ACAGAAATCA AAGCTATCCG ACGAAGCCTT 
AGAGCTGTGA GCACGACCGT GGAGGAGGAG AACAAGCTGT ACAGAAGAAC TGAGCGAGCG 
CTTCATCACC TGCTCACCTA CAAACACTTG TCGGCCATTC TGGATGCGTT GAAGCACCTG 
GAGGTCGTTA CCAGACTCTC TCCACTTTGC TGTGAGAATA TGGCTGAAAG CGGAGCGGTA 
TCTACCATCT TTGTTGTGAT CCGAAGCTGT AATCGGAGTG TTCCTTGTAT GGAAGTCGTT 
GGATATGCTG TTCAAGTATT GCTGAATGTG GCAAAGTATG ATAAGACGAT CGCGGCTGTG 
TACGAGGCCG AGAACTGCGT GGACACACTG CTGGAGCTTC TGCAGGTGTA CCGAGAGAAG 
CCTGGAGACC GAGTCGCTGA GAAGAGTGCC AGCATTTTCA CAAGAACTTG CTGTTTGTTA 
GCAGTGCTGT TGAAGACAGA GCAGTGTGCT TTTGATGCAC AAAGTAGATC TAAAGTCACT 
GATCGTATTT ATCGTCTCTA CAAATTTACA GTTCCTAAGC ATAAAGTAAA TACACAGGGA 
TTGTTTGATA AGCAGAAGCA GAATTCCTGT GTAGGCTTTC CTTGTATACC AGAAAGAACT 
ATGAAGACCA GGTTAGTTTC AAGACTTAAG CCACAGTGGG TTTTGAGGCG TGATAATGTG 
GAAGAAATTA CAAACTCCCT GCAAGCTATT CAACTGGTAA TGGATACACT TGGAATTTCT 
TATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Aspm ( NM_009791.4 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_033921.3 Mus musculus abnormal spindle microtubule assembly (Aspm), mRNA.

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CloneID	OMu04856
Clone ID Related Accession (Same CDS sequence)	NM_009791.4
Accession Version	NM_009791.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 9369bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-16
Organism	Mus musculus(house mouse)
Product	abnormal spindle-like microcephaly-associated protein homolog
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK052326.1, CN664812.1, CV556405.1, BI989704.1, AC158946.4, AY971958.1 and DV662096.1. On Mar 21, 2006 this sequence version replaced NM_009791.3. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF533752.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849384, SAMN01164134 [ECO:0000348] ##Evidence-Data-END##

ATGGCGACGA TGCAGGCAGC CTCCTGCCCA GAGGAGAGGG GGCGGCGGGC GCGACCAGAT 
CCTGAGGCCG GGGACCCGTC TCCGCCGGTG CTGTTGCTCA GCCACTTCTG CGGCGTTCCC 
TTCCTCTGCT TCGGGGATGT CCGCGTGGGC ACGTCGCGGA CGCGGTCTCT GGTCCTGCAC 
AACCCGCACG AGGAACCTCT GCAGGTGGAG CTGTCGCTGC TGCGCGCCGC AGGCCAGGGC 
TTCAGCGTGG CGCCGAACCG CTGCGAGCTG AAGCCTAAAG AAAAACTTAC CATTTCCGTT 
ACCTGGACGC CACTGCGAGA AGGGGGAGTG AGGGAGATTG TCACATTTCT TGTAAATGAT 
TTCCTGAAGC ACCAGGCTAT ATTACTAGGA AATGCAGAAG AGCCTAAGAA GAAAAAGAGA 
AGTCTTTGGA ATACCAGTAA GAAGATTCCA GCCTCTTCAA AACATACAAA AAGGACTTCC 
AAAAACCAAC ATTTTAATGA ATCATTTACT ATTTCACAAA AAGACAGAAT TAGGAGCCCA 
CTGCAGCCTT GTGAAAATCT GGCTATGAGT GAATGCTCTT CCCCAACAGA AAACAAAGTC 
CCCACCCCAT CCATTAGTCC TATTAGAGAA TGCCAGAGTG AAACTTGCTT GCCACTGTTT 
TTACGCGAGT CCACTGCCTA TTCATCTCTT CATGAATCTG AAAATACACA AAATTTAAAA 
GTACAAGATG CCAGCATTTC ACAAACTTTT GATTTTAATG AGGAAGTCGC AAATGAAACT 
TTTATTAATC CCATTAGTGT CTGTCACCAG AGTGAAGGGG ATAGGAAACT CACGCTTGCC 
CCAAACTGTT CTTCACCTTT GAATAGTACA CAGACCCAAA TACACTTTCT AAGTCCAGAT 
TCTTTTGTAA ACAACAGGTA TACGTCTGAT AATGACCTGA AATCAATGAA AAATGTTTTA 
TCAGACACAT TTAGGAAAGA TCCCGCAGAG TCTGTATGTC TGGAATCCCA AACTGTCCAT 
GAAGTTTGCC AAACAATCTT AAGCCCAGAT TCTTTTTTAA ATGATAATTA TGGACTTAAA 
AAAGGGCTAA ACTTCAAGTC CGTCAATCCC GTTTTGTCGC CAACACAATT TGTAAAGGAT 
AGCATGGGAC ACGTAGGTCA GCAAACGGGT AAGTCAAATG AAGCTTCTCA GGATTGGAGA 
ATAAATGAAG GGTTGGCGTA TACCCCCGAG TGTCAGCATG CACAGACTCC CAGCAGCAGA 
TCTGAAAAAC AAAACCCTGT AGAAGTGAAG CCACATACGT ATGATTTTAC AAAGCAAAAG 
CCCAAGATTT CTGAGTTTCA GGATGCTTTT TGTCACCAGA GTAAACAACC GCACAAGAGA 
CGCCCAATTC TTTCTGCCAC TGTTACGAAA AGGAAACCCA CCAATGCCAG AGAAAAGCTA 
CCTGAGATTA ACAAGCCAGA CGCAAAAAGA TGTCTCGAAG GTCTAGTAGG TGAACGTGGA 
AAAGAAGTGG GTAGCCTGAG AGAGAAAGGT TTTCATTCTT CTCTTCCCGT TGTGGAGCCA 
GGAGTGAGTA AAGCTCTGAG CTACAGGGAT GAAGTAACGC CTGCAACTGT TGTCGTTGCT 
CGGAAAAGAA AGAGCCACGG AACAGTGGGA GATGCAAATG GAAAAGTAGC AGCTGAAGAA 
TGGATGGACA TGTGTGAAGT CAAACGAATC CATTTTTCTC CTCTGGAATC TACACCCTCA 
ACTGTTGCAA GAACCACAAA GAAAGAAGGG CATACCTCAA AGCGGATAAG CAGCCTGGAG 
CGGTCAGGCC TGAAGAAGAA AATGGATTCA TCAATATTAA AAACACCTCT TTCTAAGACA 
AAGAAAAAGA GAAGATCCAT TGTTGCTGTA GCACAGTCTC ATTTGACCTT TATAAAGCCA 
TTAAAAGCAG CTATTCCTAG ACACCCAATG CCGTTTGCTG CAAAGAACAT GTTCTATGAC 
GAACGCTGGA AGGAAAAGCA GGAACAGGGC TTCACTTGGT GGCTAAATTA TATATTAACA 
CCTGATGACT TCACTGTAAA AACCAATGTT TCTAAAGTGA ACGCTGCCAG CCTTGTTTTG 
GGAGCGGAAA GCCAACATAA AATAAGTGTC CCGAAAGCAC CTACTAAAGA GGAAGTGTCT 
CTCAGAGCTT ATACAGCCAG CTGCAGGCTG AATAGATTAC GCCGTACAGC TTGCTCCTTG 
TTTACTTCTG AAAAAATGGT TAAAGCTATT AAAAAGGTTG AAATCGAAAT TGAAGTTGGG 
CGGCTACTTG TTCGAAAAGA CCGACACCTC TGGAAAGATA TTGGACAACG CCAAAAAGTC 
CTGAATTGGC TCTTGTCATA TAATCCTCTG TGGCTTCGAA TTGGTCTGGA GACAGTTTTT 
GGAGAACTGA TCCCTCTGGC AGACAACAGT GACGTCACGG GATTGGCGAT GTTCATTCTG 
AATCGCTTAC TTTGGAATCC TGACATAGCA GCTGAATATA GACACCCCAC TGTTCCTCTC 
CTGTTTCGGG ATGGTCATGA AGCAGCCTTG TCTAAGTTCA CATTGAAAAA ATTGCTGTTG 
TTGATCTGTT TCCTTGATCA TGCTAAAATT TCCAGACTTA TTGATCATGA TCCTTGTCTC 
TTCTGTAAAG ATGCTGAATT CAAGGCTAGC AAAGAGCTTC TTCTAGCCTT TTCACGGGAT 
TTCCTCAGTG GTGAAGGTGA CCTTTCTCGT CACCTTAGCT TCTTGGGGTT ACCTGTGAGC 
CACGTTCAGA CACCACTTGA TGAATTTGAT TTTGCTGTTA CAAATCTTGC AGTGGACTTG 
CAGTGTGGGG TTCGCCTTGT GCGAACTGTG GAGCTTCTCA CACAGAACTG GAATCTGTCC 
GACAAACTCA GGATCCCTGC AATAAGTCGA GTTCAAAAGA TGCACAATGT TGATCTCGTC 
CTTCAGGTTC TCAAGTCTCG GGGAGTTCCA CTGACTGACG AGCATGGGAG CGCTATCTCG 
TCTAAGGATG TTGTGGACAG GCACAGAGAG AAAACCCTTG GGTTGCTTTG GAAAATAGCA 
CTGGCTTTCC AGGTGGATAT TTCCCTTAAT TTAGATCAAT TAAAAGAAGA AATTGATTTT 
CTAAAGCACA CACACAGTAT AAAGAGAGCT ATGTCTGCAC TAACATGCCC TTCACAAGCT 
ATTACTAATA AGCAAAGAGA CAAAAGGATT AGTGGGAACT TTGAACGCTA TGGTGACAGT 
GTGCAGTTGT TGATGGATTG GGTAAATGCA GTTTGTGCCT TCTATAATAA AAAGGTGGAG 
AATTTTACAG TGTCTTTCTC AGATGGCCGC ATACTATGCT ATTTGATCCA CCATTACCAC 
CCGTGCTATG TGCCTTTCGA TGCTATATGC CAGCGCACCA GTCAGTCCGT GGCCTGCGCA 
CAGACTGGTT CAGTGGTATT GAATTCTTCA TCGGAATCTG AAGGAGGTTG TCTGGATCTG 
TCTCTTGAAG CGCTTGATCA CGAAAGTACT CCAGAAATGT ACAAAGAGCT TCTAGAAAAC 
GAGAAGAAAA ATTTCCATTT GGTGCGATCT GCAGCTAGGG ATCTGGGAGG AATACCTGCT 
ATGATTCATC ATTCAGACAT GTCAAATACA ATTCCAGACG AAAAGGTGGT TATCACCTAT 
TTGTCATTTC TTTGTGCAAG GCTTTTGGAT CTCCGTAAAG AAATAAGAGC TGCTCGACTT 
ATACAGACAA CATGGAGAAA ATATAAACTA AAAAGAGATC TGAAACACCA TCAGGAAAGA 
GACAAAGCTG CTAGAGTTAT TCAGTCAGTT GTTCTCAATT TTCTAAGTAG ACGAAGATTG 
CAAAAAAACG TCAGTGCAGC TCTGGTCATT CAGAAATGCT GGCGAAGAGT CTCAGCGCAA 
AGGAAGTTGC GGATGCTGAA AAATGAAAAA CTGGCAAAAC TTCAAAATAA ATCAGCAGTG 
CTCATTCAGG CTTATTGGAG AAGATACTCC ACCAGAAAAC GGTTTCTGAG GTTGAAGCAC 
TATTCAGTCA TCCTTCAGTC TAGGATAAGA ATGAAAATTG CTCTTACATC TTACAAACGA 
TATCTTTGGG CCACAGTTAC AATTCAGAGG CATTGGCGTG CCTATTTAAG TAGAAAACGA 
GATCAGCAAA TATTTAGGAA GCTGAAATCC TCATCCCTTG TAATCCAGTT TATGTTCAGA 
AGGTGGAAGC GACGCAAGCT GCAGTTACAA ACCAAAGCTG CAGTAACATT GCAGCGAGCT 
TTTAGAGAAT GGCATCTCAG GAAACAAATA CGAGAAAGAT CCGCTGTTGT GATACAGTCA 
TGGTACAGAA TGCACAGGGA ATTGCAGAAA TACATTTATA TTAGGTCTTG TGTTATTGTC 
ATCCAGAGAA GAGTTCGGTG CTTTCAAGCC CAAAAGTTAT ATAAGAGAAG GAAAGATGCC 
ATCCTGACTC TCCAGAAACA TTACAGGGCA CGTCAGAAGG GGAAGCTAGC ACACGCTGAC 
TATTTGCAGA AACGAGCTGC CACCATTCGA CTGCAGGCTG CATTCAGGGG GATGAAGGCT 
CGCCATTCAT ACAGGTTGCA GATTGGAGCT GCCTGTGTTC TTCAGTCCTA TTGGAGAATG 
AGACAGGAAA GAGTTAGATT TTTAAACCTT AAAAAGATGG TTATTAAGTT GCAAGCACAC 
ATAAGAAAAT ACCAACAACT ACAGAAATAT AAGAAGATAA AGAAAGCAGC CATTACAATT 
CAGACTCATT TCCGAGCTTC CATTTCAGCC AGGAGGGTTC TAGCATCTTA TCAGAAAACA 
AGGTCTTCAG TCATTGTTCT ACAGTCTGCA TGTAGAGGGA TGCAAGCCAG AAAAGCATTT 
CGCCATGCTT TAGCATCCGT TATAAAGATT CAGTCATATT ATCGGGCTTA TATATGTAGG 
AAGACATTTC AGAACTTCAA AAATGCCACA ATAAAGCTGC AGTCAATTGT CAAAATGAAA 
CAATCACGGA AGCAGTATTT ACAAATAAGA GCAGCTGCAC TGTTTATCCA GCGGTGGTAC 
CGTTCCCAAA AACTGGCCTC ACAAAAGAGA AAAGAGTACA TACAGGTGCG TGAATCCTGT 
ATCAAACTGC AGTCACACTT TAGAGGATGC CTGGTCAGAA AGCAGTTGAG GTTGCAATGT 
AAAGCTGCCA TTTCTCTACA GTCTTATTTC AGAATGAGAA CAGCGAGGCA GCGCTACCTG 
AAGATGTGCA AGGCGGCTCT TGTCATTCAG AGTTTCTACT GTGCCTACAG AGCACAAATC 
AGTCAAAGGA AGAACTTCTT GCAGGTCAAA AGAGCAGCTA TTTGTTTGCA GGCAGCTTAC 
AGAGGCTGCA AAGTACGCCG GCAAATCAAG CAGCAGTCTA CGGCTGCTGT TACAATTCAG 
AGGGTTTTTA GAGGCCACAG TCAGAGGATG AAATACCAGA CTATGCTTCA GTCTGCTGTG 
AAGATTCAGA GATGGTACAG GGCACAGAAG GTTGCTTATG ACATGAGAAT TCAATTTCTA 
AAGACTAGAG AGGCTGTGGT TTGCCTCCAG TCTGCCTACC GTGGCTGGCA GGTTCGGCAA 
CAGCTCAGGA GGCAACATGA GGCTGCAGTG AAGATCCAGT CTACGTTTAG AATGGCCGTG 
GCCCAGCAAC AGTATAAACT ACTAAGAGCA GCTGCAGCAG TCATCCAGCA GCATGTGAGG 
GCACGGGCTG CAGGGAAGAG GCAGCACTTG GCGTACATCC AGCTCCGTCA TGCAGCTCTG 
GTGTTTCAGG CTGCATGGAA GGGAAAGATG CTGAGAAGAC AGATTGCCCG GCAGCATCAA 
TGCGCTGCCC TCATACAGTC ATATTACAGG ATGCACATTC AGCGCAGGAA GTGGAGCATC 
ATGAAAACCG CTGCCCTTCA GATTCAGCTG TGCTATAGGG CTTACAAAGT TGGAAAAGAA 
CAGCGCCACT TATACTTGAA AACAAAAGCA GCTGTAGTAA CTTTACAGTC AGCTTACCGT 
GGTATGAAAG TGAGAAAGCG GGTAGCTGAA TGCCACAAAG CAGCGGTCAC TATACAGTCT 
AAATTTAGAG CCTACAGAAC ACAAAAGAAG TACACGACCT ATAGAACTTC AGCTATTGTA 
ATTCAGAGGT GGTACCGGAA TATTAAAATT ACAACCCAGC AACATCAGGA ATACCTTAAC 
TTAAGGAGGG CAGCAGTTCA AGTCCAGGCT GCGTATAGAG GCATTAGAGT GAGGAGACGC 
ATTCAACACA TGCACATGGC GGCCACACTT ATTGAAGCCA TGTTTAAGAT GCGTCAGTCA 
AGAGTAAGGT ACCTCAAGAT GAGAACAGCA GCTCTTATAA TTCAGGTGAG ATACAGAGCG 
TATTATCTAG GTAAGATTCA GCATGAGAAG TACCTGAGGA CTTTGAAGGC TATTAAAACC 
CTTCAAGCTG GAGTCAGAGG AGCAAGAGTG AGGCGGACTG TAAGAAAGAT GCATTTCGCA 
GCAACACTCA TTCAGTCACA TTTTAGGGGA CACAGGCAGC AAACTTACTT TCATCGGTTG 
AGGAAGGCGG CAACCATGGT GCAGCAGAGA TACCGAGCAG TGAAAGAAGG AAGCGCTGAG 
TTTCAGAGGT ACAGCAGACT AAGGCGTTCT GTACTCCTCA TCCAGGCTGC CTTCAGGGGA 
CTGAGAACTA GGAGACACTT AAAAGCTATG CACCTGGCTG CTACTCTCAT TCAGAGGAGA 
TTCAGGACTT TCGCGATGAG AAGGAAGTTC CTTTCCCTCA GGAAAACTGC CATTTGGATT 
CAGAGACAGT ACCGTGCAAG ACTTTATGCC AAGTATTCCC GACAACAGTT ACTGTTGGAA 
AAGGCAGTTA TAAAGATCCA GTCATCATAC AGAGGATGGG TGGTAAGGAA AAGGGTGCAA 
AAGATGCACA GGGCGGCTAC TGTGATTCAG GCCACTTTCC GGATGCACGG AGCCTATATG 
AGGTACCAGC ATTTGAAGCG GGCCTCAGTT GTGATCCAAG TACACACAGC TGCAGAACTG 
CAGAGGCAAA AGCATGCTGC TGTAATCCTT CAGGCTGCCG TTAGGGGGAT GAAAACCAGA 
AGTCATCTGA AGACTATGCA TTCTTCTGCA ACCCTTATTC AGAGTCAATT TAGAGCTTTT 
ATAGTAAGGA GACGATTCAT TGCTCTCAGA AAAGCTGCTA TATTTGTGCA GAGGAAATTC 
CGAGCCACCC TTTATGCCAA GCATAAATTG CACCAATTCT TGCAGTTACG AAAGGCAGCC 
ATTACCATAC AGTCCTCTTA TCGAAGACTG ATGGTACAAA AGAAGTTACA AGAAATGCAC 
AGGGCTGCAG CTCTCATCCA GGCTACTTTT AGAATGCACA GAACTTACGT TGCATTTCAC 
ATTTGGAAAT GTGCCTCGAT TAGAATTCAG CAGTGTTATC GAACATACAG AACTATAAAA 
TTACAAAAAG AAAAACTTAT CAGAGAAGAA CAACACAGTG CTGCTGTCCT AATACAAAGC 
ACGTACAGGA TGTACAGGCA GCGTTGCTTC TATCAACAGC GTCGGTGGGC AGCAAAGGTA 
ATACAAAAAA CATACAGAGC AAATAAAAGA CGGCAAGATC TGCTCTATGT GTGTAAGGAA 
GAAACACCTC TTCTTCAGAT GCATTTTCAG GGTTTAAACA CTGCAAAACA AGGTCGGCAG 
CAGCATGGGG CCGCTATGAT TACACAGAAG CATTTTAGAG CCTTTAAAGC AAGGAGGCTG 
ATGGAGGCCG AGAGAGGCTT CCAGGCTGGA TGTAGAAAAT ATAAAGCTAA GAAATACTTA 
TCCAAAGTGG AAGCTGCCTG CAGGATTCAA GCTTGGTATA GGCGTTGGAG AGCGCACAAG 
AAATATCTAA CTTTATTGAA AGCTGTTAAC ATTATTGAAG GTTACCTCTC TGCACAGCTG 
GCGAGAAGGA GGTTCTTGAA GATGAGGGCG GCAGCTATTA TAATTCAAAG GAAATGGAGA 
GCTACACTTT CTGTAAGAGG AGCTCGTGAG AATTTAAAAA GACATCGAGC TGCTTGTGTG 
ATTCAAGCCC ACTTTAGGGG CTATCAGGCA AGGCAGTCCT TCCTGCAGCA GAGATCTGCT 
GTTTTAATCA TACAGAGACA TGTACGAGCC ATGGTGGCTG CGAAGCAGGA AAGGATAAAG 
TATATCAAAT TGAAAAAGTC TACGGTTGTT GTACAAGCCT TGGTGCGTGG CTGGTTAGTA 
AGGAAAAGGG TTTCAGAGCA GAAAGCCAAA ACTCGGCTTT TCCACTTTAC AGCAGCTGCC 
TACTGTCACA TGTGTGCTCT TAAAATCCAA CGAGCTTATC GGCTCCACGT GACCTTGAGG 
AACGCTAAGA AGCACATGGA TTCAGTCATC TTCATTCAGA GATGGTTTCG AAAAAGATTA 
CAACGAAAGA GGTTCATTGA ACAGTACCAC AAGATCCTAA GCACTCGACG TGAAGCTCAT 
GCATGTTGGC TCCAGCAGGA CAGGGCTGCA TCCGTGATAC AGAAGGCGGT ACGCCGCTTT 
CTCCTCTGCA GAAGACAGGA AAAGATCACT AGCTGCGCCA CTAGAATTCA GGCATTATGG 
AGAGGCTATT CTTGGAGGAA GAAAAATGAC CACACAGAAA TCAAAGCTAT CCGACGAAGC 
CTTAGAGCTG TGAGCACGAC CGTGGAGGAG GAGAACAAGC TGTACAGAAG AACTGAGCGA 
GCGCTTCATC ACCTGCTCAC CTACAAACAC TTGTCGGCCA TTCTGGATGC GTTGAAGCAC 
CTGGAGGTCG TTACCAGACT CTCTCCACTT TGCTGTGAGA ATATGGCTGA AAGCGGAGCG 
GTATCTACCA TCTTTGTTGT GATCCGAAGC TGTAATCGGA GTGTTCCTTG TATGGAAGTC 
GTTGGATATG CTGTTCAAGT ATTGCTGAAT GTGGCAAAGT ATGATAAGAC GATCGCGGCT 
GTGTACGAGG CCGAGAACTG CGTGGACACA CTGCTGGAGC TTCTGCAGGT GTACCGAGAG 
AAGCCTGGAG ACCGAGTCGC TGAGAAGAGT GCCAGCATTT TCACAAGAAC TTGCTGTTTG 
TTAGCAGTGC TGTTGAAGAC AGAGCAGTGT GCTTTTGATG CACAAAGTAG ATCTAAAGTC 
ACTGATCGTA TTTATCGTCT CTACAAATTT ACAGTTCCTA AGCATAAAGT AAATACACAG 
GGATTGTTTG ATAAGCAGAA GCAGAATTCC TGTGTAGGCT TTCCTTGTAT ACCAGAAAGA 
ACTATGAAGA CCAGGTTAGT TTCAAGACTT AAGCCACAGT GGGTTTTGAG GCGTGATAAT 
GTGGAAGAAA TTACAAACTC CCTGCAAGCT ATTCAACTGG TAATGGATAC ACTTGGAATT 
TCTTATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_033921.3
CDS	160..9528
Translation	MATMQAASCPEERGRRARPDPEAGDPSPPVLLLSHFCGVPFLCFGDVRVGTSRTRSLVLHNPHEEPLQVELSLLRAAGQGFSVAPNRCELKPKEKLTISVTWTPLREGGVREIVTFLVNDFLKHQAILLGNAEEPKKKKRSLWNTSKKIPASSKHTKRTSKNQHFNESFTISQKDRIRSPLQPCENLAMSECSSPTENKVPTPSISPIRECQSETCLPLFLRESTAYSSLHESENTQNLKVQDASISQTFDFNEEVANETFINPISVCHQSEGDRKLTLAPNCSSPLNSTQTQIHFLSPDSFVNNRYTSDNDLKSMKNVLSDTFRKDPAESVCLESQTVHEVCQTILSPDSFLNDNYGLKKGLNFKSVNPVLSPTQFVKDSMGHVGQQTGKSNEASQDWRINEGLAYTPECQHAQTPSSRSEKQNPVEVKPHTYDFTKQKPKISEFQDAFCHQSKQPHKRRPILSATVTKRKPTNAREKLPEINKPDAKRCLEGLVGERGKEVGSLREKGFHSSLPVVEPGVSKALSYRDEVTPATVVVARKRKSHGTVGDANGKVAAEEWMDMCEVKRIHFSPLESTPSTVARTTKKEGHTSKRISSLERSGLKKKMDSSILKTPLSKTKKKRRSIVAVAQSHLTFIKPLKAAIPRHPMPFAAKNMFYDERWKEKQEQGFTWWLNYILTPDDFTVKTNVSKVNAASLVLGAESQHKISVPKAPTKEEVSLRAYTASCRLNRLRRTACSLFTSEKMVKAIKKVEIEIEVGRLLVRKDRHLWKDIGQRQKVLNWLLSYNPLWLRIGLETVFGELIPLADNSDVTGLAMFILNRLLWNPDIAAEYRHPTVPLLFRDGHEAALSKFTLKKLLLLICFLDHAKISRLIDHDPCLFCKDAEFKASKELLLAFSRDFLSGEGDLSRHLSFLGLPVSHVQTPLDEFDFAVTNLAVDLQCGVRLVRTVELLTQNWNLSDKLRIPAISRVQKMHNVDLVLQVLKSRGVPLTDEHGSAISSKDVVDRHREKTLGLLWKIALAFQVDISLNLDQLKEEIDFLKHTHSIKRAMSALTCPSQAITNKQRDKRISGNFERYGDSVQLLMDWVNAVCAFYNKKVENFTVSFSDGRILCYLIHHYHPCYVPFDAICQRTSQSVACAQTGSVVLNSSSESEGGCLDLSLEALDHESTPEMYKELLENEKKNFHLVRSAARDLGGIPAMIHHSDMSNTIPDEKVVITYLSFLCARLLDLRKEIRAARLIQTTWRKYKLKRDLKHHQERDKAARVIQSVVLNFLSRRRLQKNVSAALVIQKCWRRVSAQRKLRMLKNEKLAKLQNKSAVLIQAYWRRYSTRKRFLRLKHYSVILQSRIRMKIALTSYKRYLWATVTIQRHWRAYLSRKRDQQIFRKLKSSSLVIQFMFRRWKRRKLQLQTKAAVTLQRAFREWHLRKQIRERSAVVIQSWYRMHRELQKYIYIRSCVIVIQRRVRCFQAQKLYKRRKDAILTLQKHYRARQKGKLAHADYLQKRAATIRLQAAFRGMKARHSYRLQIGAACVLQSYWRMRQERVRFLNLKKMVIKLQAHIRKYQQLQKYKKIKKAAITIQTHFRASISARRVLASYQKTRSSVIVLQSACRGMQARKAFRHALASVIKIQSYYRAYICRKTFQNFKNATIKLQSIVKMKQSRKQYLQIRAAALFIQRWYRSQKLASQKRKEYIQVRESCIKLQSHFRGCLVRKQLRLQCKAAISLQSYFRMRTARQRYLKMCKAALVIQSFYCAYRAQISQRKNFLQVKRAAICLQAAYRGCKVRRQIKQQSTAAVTIQRVFRGHSQRMKYQTMLQSAVKIQRWYRAQKVAYDMRIQFLKTREAVVCLQSAYRGWQVRQQLRRQHEAAVKIQSTFRMAVAQQQYKLLRAAAAVIQQHVRARAAGKRQHLAYIQLRHAALVFQAAWKGKMLRRQIARQHQCAALIQSYYRMHIQRRKWSIMKTAALQIQLCYRAYKVGKEQRHLYLKTKAAVVTLQSAYRGMKVRKRVAECHKAAVTIQSKFRAYRTQKKYTTYRTSAIVIQRWYRNIKITTQQHQEYLNLRRAAVQVQAAYRGIRVRRRIQHMHMAATLIEAMFKMRQSRVRYLKMRTAALIIQVRYRAYYLGKIQHEKYLRTLKAIKTLQAGVRGARVRRTVRKMHFAATLIQSHFRGHRQQTYFHRLRKAATMVQQRYRAVKEGSAEFQRYSRLRRSVLLIQAAFRGLRTRRHLKAMHLAATLIQRRFRTFAMRRKFLSLRKTAIWIQRQYRARLYAKYSRQQLLLEKAVIKIQSSYRGWVVRKRVQKMHRAATVIQATFRMHGAYMRYQHLKRASVVIQVHTAAELQRQKHAAVILQAAVRGMKTRSHLKTMHSSATLIQSQFRAFIVRRRFIALRKAAIFVQRKFRATLYAKHKLHQFLQLRKAAITIQSSYRRLMVQKKLQEMHRAAALIQATFRMHRTYVAFHIWKCASIRIQQCYRTYRTIKLQKEKLIREEQHSAAVLIQSTYRMYRQRCFYQQRRWAAKVIQKTYRANKRRQDLLYVCKEETPLLQMHFQGLNTAKQGRQQHGAAMITQKHFRAFKARRLMEAERGFQAGCRKYKAKKYLSKVEAACRIQAWYRRWRAHKKYLTLLKAVNIIEGYLSAQLARRRFLKMRAAAIIIQRKWRATLSVRGARENLKRHRAACVIQAHFRGYQARQSFLQQRSAVLIIQRHVRAMVAAKQERIKYIKLKKSTVVVQALVRGWLVRKRVSEQKAKTRLFHFTAAAYCHMCALKIQRAYRLHVTLRNAKKHMDSVIFIQRWFRKRLQRKRFIEQYHKILSTRREAHACWLQQDRAASVIQKAVRRFLLCRRQEKITSCATRIQALWRGYSWRKKNDHTEIKAIRRSLRAVSTTVEEENKLYRRTERALHHLLTYKHLSAILDALKHLEVVTRLSPLCCENMAESGAVSTIFVVIRSCNRSVPCMEVVGYAVQVLLNVAKYDKTIAAVYEAENCVDTLLELLQVYREKPGDRVAEKSASIFTRTCCLLAVLLKTEQCAFDAQSRSKVTDRIYRLYKFTVPKHKVNTQGLFDKQKQNSCVGFPCIPERTMKTRLVSRLKPQWVLRRDNVEEITNSLQAIQLVMDTLGISY

Target ORF information:

RefSeq Version	NM_009791.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus abnormal spindle microtubule assembly (Aspm), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_009791.4

ORF Insert Sequence:

ATGGCGACGA TGCAGGCAGC CTCCTGCCCA GAGGAGAGGG GGCGGCGGGC GCGACCAGAT 
CCTGAGGCCG GGGACCCGTC TCCGCCGGTG CTGTTGCTCA GCCACTTCTG CGGCGTTCCC 
TTCCTCTGCT TCGGGGATGT CCGCGTGGGC ACGTCGCGGA CGCGGTCTCT GGTCCTGCAC 
AACCCGCACG AGGAACCTCT GCAGGTGGAG CTGTCGCTGC TGCGCGCCGC AGGCCAGGGC 
TTCAGCGTGG CGCCGAACCG CTGCGAGCTG AAGCCTAAAG AAAAACTTAC CATTTCCGTT 
ACCTGGACGC CACTGCGAGA AGGGGGAGTG AGGGAGATTG TCACATTTCT TGTAAATGAT 
TTCCTGAAGC ACCAGGCTAT ATTACTAGGA AATGCAGAAG AGCCTAAGAA GAAAAAGAGA 
AGTCTTTGGA ATACCAGTAA GAAGATTCCA GCCTCTTCAA AACATACAAA AAGGACTTCC 
AAAAACCAAC ATTTTAATGA ATCATTTACT ATTTCACAAA AAGACAGAAT TAGGAGCCCA 
CTGCAGCCTT GTGAAAATCT GGCTATGAGT GAATGCTCTT CCCCAACAGA AAACAAAGTC 
CCCACCCCAT CCATTAGTCC TATTAGAGAA TGCCAGAGTG AAACTTGCTT GCCACTGTTT 
TTACGCGAGT CCACTGCCTA TTCATCTCTT CATGAATCTG AAAATACACA AAATTTAAAA 
GTACAAGATG CCAGCATTTC ACAAACTTTT GATTTTAATG AGGAAGTCGC AAATGAAACT 
TTTATTAATC CCATTAGTGT CTGTCACCAG AGTGAAGGGG ATAGGAAACT CACGCTTGCC 
CCAAACTGTT CTTCACCTTT GAATAGTACA CAGACCCAAA TACACTTTCT AAGTCCAGAT 
TCTTTTGTAA ACAACAGGTA TACGTCTGAT AATGACCTGA AATCAATGAA AAATGTTTTA 
TCAGACACAT TTAGGAAAGA TCCCGCAGAG TCTGTATGTC TGGAATCCCA AACTGTCCAT 
GAAGTTTGCC AAACAATCTT AAGCCCAGAT TCTTTTTTAA ATGATAATTA TGGACTTAAA 
AAAGGGCTAA ACTTCAAGTC CGTCAATCCC GTTTTGTCGC CAACACAATT TGTAAAGGAT 
AGCATGGGAC ACGTAGGTCA GCAAACGGGT AAGTCAAATG AAGCTTCTCA GGATTGGAGA 
ATAAATGAAG GGTTGGCGTA TACCCCCGAG TGTCAGCATG CACAGACTCC CAGCAGCAGA 
TCTGAAAAAC AAAACCCTGT AGAAGTGAAG CCACATACGT ATGATTTTAC AAAGCAAAAG 
CCCAAGATTT CTGAGTTTCA GGATGCTTTT TGTCACCAGA GTAAACAACC GCACAAGAGA 
CGCCCAATTC TTTCTGCCAC TGTTACGAAA AGGAAACCCA CCAATGCCAG AGAAAAGCTA 
CCTGAGATTA ACAAGCCAGA CGCAAAAAGA TGTCTCGAAG GTCTAGTAGG TGAACGTGGA 
AAAGAAGTGG GTAGCCTGAG AGAGAAAGGT TTTCATTCTT CTCTTCCCGT TGTGGAGCCA 
GGAGTGAGTA AAGCTCTGAG CTACAGGGAT GAAGTAACGC CTGCAACTGT TGTCGTTGCT 
CGGAAAAGAA AGAGCCACGG AACAGTGGGA GATGCAAATG GAAAAGTAGC AGCTGAAGAA 
TGGATGGACA TGTGTGAAGT CAAACGAATC CATTTTTCTC CTCTGGAATC TACACCCTCA 
ACTGTTGCAA GAACCACAAA GAAAGAAGGG CATACCTCAA AGCGGATAAG CAGCCTGGAG 
CGGTCAGGCC TGAAGAAGAA AATGGATTCA TCAATATTAA AAACACCTCT TTCTAAGACA 
AAGAAAAAGA GAAGATCCAT TGTTGCTGTA GCACAGTCTC ATTTGACCTT TATAAAGCCA 
TTAAAAGCAG CTATTCCTAG ACACCCAATG CCGTTTGCTG CAAAGAACAT GTTCTATGAC 
GAACGCTGGA AGGAAAAGCA GGAACAGGGC TTCACTTGGT GGCTAAATTA TATATTAACA 
CCTGATGACT TCACTGTAAA AACCAATGTT TCTAAAGTGA ACGCTGCCAG CCTTGTTTTG 
GGAGCGGAAA GCCAACATAA AATAAGTGTC CCGAAAGCAC CTACTAAAGA GGAAGTGTCT 
CTCAGAGCTT ATACAGCCAG CTGCAGGCTG AATAGATTAC GCCGTACAGC TTGCTCCTTG 
TTTACTTCTG AAAAAATGGT TAAAGCTATT AAAAAGGTTG AAATCGAAAT TGAAGTTGGG 
CGGCTACTTG TTCGAAAAGA CCGACACCTC TGGAAAGATA TTGGACAACG CCAAAAAGTC 
CTGAATTGGC TCTTGTCATA TAATCCTCTG TGGCTTCGAA TTGGTCTGGA GACAGTTTTT 
GGAGAACTGA TCCCTCTGGC AGACAACAGT GACGTCACGG GATTGGCGAT GTTCATTCTG 
AATCGCTTAC TTTGGAATCC TGACATAGCA GCTGAATATA GACACCCCAC TGTTCCTCTC 
CTGTTTCGGG ATGGTCATGA AGCAGCCTTG TCTAAGTTCA CATTGAAAAA ATTGCTGTTG 
TTGATCTGTT TCCTTGATCA TGCTAAAATT TCCAGACTTA TTGATCATGA TCCTTGTCTC 
TTCTGTAAAG ATGCTGAATT CAAGGCTAGC AAAGAGCTTC TTCTAGCCTT TTCACGGGAT 
TTCCTCAGTG GTGAAGGTGA CCTTTCTCGT CACCTTAGCT TCTTGGGGTT ACCTGTGAGC 
CACGTTCAGA CACCACTTGA TGAATTTGAT TTTGCTGTTA CAAATCTTGC AGTGGACTTG 
CAGTGTGGGG TTCGCCTTGT GCGAACTGTG GAGCTTCTCA CACAGAACTG GAATCTGTCC 
GACAAACTCA GGATCCCTGC AATAAGTCGA GTTCAAAAGA TGCACAATGT TGATCTCGTC 
CTTCAGGTTC TCAAGTCTCG GGGAGTTCCA CTGACTGACG AGCATGGGAG CGCTATCTCG 
TCTAAGGATG TTGTGGACAG GCACAGAGAG AAAACCCTTG GGTTGCTTTG GAAAATAGCA 
CTGGCTTTCC AGGTGGATAT TTCCCTTAAT TTAGATCAAT TAAAAGAAGA AATTGATTTT 
CTAAAGCACA CACACAGTAT AAAGAGAGCT ATGTCTGCAC TAACATGCCC TTCACAAGCT 
ATTACTAATA AGCAAAGAGA CAAAAGGATT AGTGGGAACT TTGAACGCTA TGGTGACAGT 
GTGCAGTTGT TGATGGATTG GGTAAATGCA GTTTGTGCCT TCTATAATAA AAAGGTGGAG 
AATTTTACAG TGTCTTTCTC AGATGGCCGC ATACTATGCT ATTTGATCCA CCATTACCAC 
CCGTGCTATG TGCCTTTCGA TGCTATATGC CAGCGCACCA GTCAGTCCGT GGCCTGCGCA 
CAGACTGGTT CAGTGGTATT GAATTCTTCA TCGGAATCTG AAGGAGGTTG TCTGGATCTG 
TCTCTTGAAG CGCTTGATCA CGAAAGTACT CCAGAAATGT ACAAAGAGCT TCTAGAAAAC 
GAGAAGAAAA ATTTCCATTT GGTGCGATCT GCAGCTAGGG ATCTGGGAGG AATACCTGCT 
ATGATTCATC ATTCAGACAT GTCAAATACA ATTCCAGACG AAAAGGTGGT TATCACCTAT 
TTGTCATTTC TTTGTGCAAG GCTTTTGGAT CTCCGTAAAG AAATAAGAGC TGCTCGACTT 
ATACAGACAA CATGGAGAAA ATATAAACTA AAAAGAGATC TGAAACACCA TCAGGAAAGA 
GACAAAGCTG CTAGAGTTAT TCAGTCAGTT GTTCTCAATT TTCTAAGTAG ACGAAGATTG 
CAAAAAAACG TCAGTGCAGC TCTGGTCATT CAGAAATGCT GGCGAAGAGT CTCAGCGCAA 
AGGAAGTTGC GGATGCTGAA AAATGAAAAA CTGGCAAAAC TTCAAAATAA ATCAGCAGTG 
CTCATTCAGG CTTATTGGAG AAGATACTCC ACCAGAAAAC GGTTTCTGAG GTTGAAGCAC 
TATTCAGTCA TCCTTCAGTC TAGGATAAGA ATGAAAATTG CTCTTACATC TTACAAACGA 
TATCTTTGGG CCACAGTTAC AATTCAGAGG CATTGGCGTG CCTATTTAAG TAGAAAACGA 
GATCAGCAAA TATTTAGGAA GCTGAAATCC TCATCCCTTG TAATCCAGTT TATGTTCAGA 
AGGTGGAAGC GACGCAAGCT GCAGTTACAA ACCAAAGCTG CAGTAACATT GCAGCGAGCT 
TTTAGAGAAT GGCATCTCAG GAAACAAATA CGAGAAAGAT CCGCTGTTGT GATACAGTCA 
TGGTACAGAA TGCACAGGGA ATTGCAGAAA TACATTTATA TTAGGTCTTG TGTTATTGTC 
ATCCAGAGAA GAGTTCGGTG CTTTCAAGCC CAAAAGTTAT ATAAGAGAAG GAAAGATGCC 
ATCCTGACTC TCCAGAAACA TTACAGGGCA CGTCAGAAGG GGAAGCTAGC ACACGCTGAC 
TATTTGCAGA AACGAGCTGC CACCATTCGA CTGCAGGCTG CATTCAGGGG GATGAAGGCT 
CGCCATTCAT ACAGGTTGCA GATTGGAGCT GCCTGTGTTC TTCAGTCCTA TTGGAGAATG 
AGACAGGAAA GAGTTAGATT TTTAAACCTT AAAAAGATGG TTATTAAGTT GCAAGCACAC 
ATAAGAAAAT ACCAACAACT ACAGAAATAT AAGAAGATAA AGAAAGCAGC CATTACAATT 
CAGACTCATT TCCGAGCTTC CATTTCAGCC AGGAGGGTTC TAGCATCTTA TCAGAAAACA 
AGGTCTTCAG TCATTGTTCT ACAGTCTGCA TGTAGAGGGA TGCAAGCCAG AAAAGCATTT 
CGCCATGCTT TAGCATCCGT TATAAAGATT CAGTCATATT ATCGGGCTTA TATATGTAGG 
AAGACATTTC AGAACTTCAA AAATGCCACA ATAAAGCTGC AGTCAATTGT CAAAATGAAA 
CAATCACGGA AGCAGTATTT ACAAATAAGA GCAGCTGCAC TGTTTATCCA GCGGTGGTAC 
CGTTCCCAAA AACTGGCCTC ACAAAAGAGA AAAGAGTACA TACAGGTGCG TGAATCCTGT 
ATCAAACTGC AGTCACACTT TAGAGGATGC CTGGTCAGAA AGCAGTTGAG GTTGCAATGT 
AAAGCTGCCA TTTCTCTACA GTCTTATTTC AGAATGAGAA CAGCGAGGCA GCGCTACCTG 
AAGATGTGCA AGGCGGCTCT TGTCATTCAG AGTTTCTACT GTGCCTACAG AGCACAAATC 
AGTCAAAGGA AGAACTTCTT GCAGGTCAAA AGAGCAGCTA TTTGTTTGCA GGCAGCTTAC 
AGAGGCTGCA AAGTACGCCG GCAAATCAAG CAGCAGTCTA CGGCTGCTGT TACAATTCAG 
AGGGTTTTTA GAGGCCACAG TCAGAGGATG AAATACCAGA CTATGCTTCA GTCTGCTGTG 
AAGATTCAGA GATGGTACAG GGCACAGAAG GTTGCTTATG ACATGAGAAT TCAATTTCTA 
AAGACTAGAG AGGCTGTGGT TTGCCTCCAG TCTGCCTACC GTGGCTGGCA GGTTCGGCAA 
CAGCTCAGGA GGCAACATGA GGCTGCAGTG AAGATCCAGT CTACGTTTAG AATGGCCGTG 
GCCCAGCAAC AGTATAAACT ACTAAGAGCA GCTGCAGCAG TCATCCAGCA GCATGTGAGG 
GCACGGGCTG CAGGGAAGAG GCAGCACTTG GCGTACATCC AGCTCCGTCA TGCAGCTCTG 
GTGTTTCAGG CTGCATGGAA GGGAAAGATG CTGAGAAGAC AGATTGCCCG GCAGCATCAA 
TGCGCTGCCC TCATACAGTC ATATTACAGG ATGCACATTC AGCGCAGGAA GTGGAGCATC 
ATGAAAACCG CTGCCCTTCA GATTCAGCTG TGCTATAGGG CTTACAAAGT TGGAAAAGAA 
CAGCGCCACT TATACTTGAA AACAAAAGCA GCTGTAGTAA CTTTACAGTC AGCTTACCGT 
GGTATGAAAG TGAGAAAGCG GGTAGCTGAA TGCCACAAAG CAGCGGTCAC TATACAGTCT 
AAATTTAGAG CCTACAGAAC ACAAAAGAAG TACACGACCT ATAGAACTTC AGCTATTGTA 
ATTCAGAGGT GGTACCGGAA TATTAAAATT ACAACCCAGC AACATCAGGA ATACCTTAAC 
TTAAGGAGGG CAGCAGTTCA AGTCCAGGCT GCGTATAGAG GCATTAGAGT GAGGAGACGC 
ATTCAACACA TGCACATGGC GGCCACACTT ATTGAAGCCA TGTTTAAGAT GCGTCAGTCA 
AGAGTAAGGT ACCTCAAGAT GAGAACAGCA GCTCTTATAA TTCAGGTGAG ATACAGAGCG 
TATTATCTAG GTAAGATTCA GCATGAGAAG TACCTGAGGA CTTTGAAGGC TATTAAAACC 
CTTCAAGCTG GAGTCAGAGG AGCAAGAGTG AGGCGGACTG TAAGAAAGAT GCATTTCGCA 
GCAACACTCA TTCAGTCACA TTTTAGGGGA CACAGGCAGC AAACTTACTT TCATCGGTTG 
AGGAAGGCGG CAACCATGGT GCAGCAGAGA TACCGAGCAG TGAAAGAAGG AAGCGCTGAG 
TTTCAGAGGT ACAGCAGACT AAGGCGTTCT GTACTCCTCA TCCAGGCTGC CTTCAGGGGA 
CTGAGAACTA GGAGACACTT AAAAGCTATG CACCTGGCTG CTACTCTCAT TCAGAGGAGA 
TTCAGGACTT TCGCGATGAG AAGGAAGTTC CTTTCCCTCA GGAAAACTGC CATTTGGATT 
CAGAGACAGT ACCGTGCAAG ACTTTATGCC AAGTATTCCC GACAACAGTT ACTGTTGGAA 
AAGGCAGTTA TAAAGATCCA GTCATCATAC AGAGGATGGG TGGTAAGGAA AAGGGTGCAA 
AAGATGCACA GGGCGGCTAC TGTGATTCAG GCCACTTTCC GGATGCACGG AGCCTATATG 
AGGTACCAGC ATTTGAAGCG GGCCTCAGTT GTGATCCAAG TACACACAGC TGCAGAACTG 
CAGAGGCAAA AGCATGCTGC TGTAATCCTT CAGGCTGCCG TTAGGGGGAT GAAAACCAGA 
AGTCATCTGA AGACTATGCA TTCTTCTGCA ACCCTTATTC AGAGTCAATT TAGAGCTTTT 
ATAGTAAGGA GACGATTCAT TGCTCTCAGA AAAGCTGCTA TATTTGTGCA GAGGAAATTC 
CGAGCCACCC TTTATGCCAA GCATAAATTG CACCAATTCT TGCAGTTACG AAAGGCAGCC 
ATTACCATAC AGTCCTCTTA TCGAAGACTG ATGGTACAAA AGAAGTTACA AGAAATGCAC 
AGGGCTGCAG CTCTCATCCA GGCTACTTTT AGAATGCACA GAACTTACGT TGCATTTCAC 
ATTTGGAAAT GTGCCTCGAT TAGAATTCAG CAGTGTTATC GAACATACAG AACTATAAAA 
TTACAAAAAG AAAAACTTAT CAGAGAAGAA CAACACAGTG CTGCTGTCCT AATACAAAGC 
ACGTACAGGA TGTACAGGCA GCGTTGCTTC TATCAACAGC GTCGGTGGGC AGCAAAGGTA 
ATACAAAAAA CATACAGAGC AAATAAAAGA CGGCAAGATC TGCTCTATGT GTGTAAGGAA 
GAAACACCTC TTCTTCAGAT GCATTTTCAG GGTTTAAACA CTGCAAAACA AGGTCGGCAG 
CAGCATGGGG CCGCTATGAT TACACAGAAG CATTTTAGAG CCTTTAAAGC AAGGAGGCTG 
ATGGAGGCCG AGAGAGGCTT CCAGGCTGGA TGTAGAAAAT ATAAAGCTAA GAAATACTTA 
TCCAAAGTGG AAGCTGCCTG CAGGATTCAA GCTTGGTATA GGCGTTGGAG AGCGCACAAG 
AAATATCTAA CTTTATTGAA AGCTGTTAAC ATTATTGAAG GTTACCTCTC TGCACAGCTG 
GCGAGAAGGA GGTTCTTGAA GATGAGGGCG GCAGCTATTA TAATTCAAAG GAAATGGAGA 
GCTACACTTT CTGTAAGAGG AGCTCGTGAG AATTTAAAAA GACATCGAGC TGCTTGTGTG 
ATTCAAGCCC ACTTTAGGGG CTATCAGGCA AGGCAGTCCT TCCTGCAGCA GAGATCTGCT 
GTTTTAATCA TACAGAGACA TGTACGAGCC ATGGTGGCTG CGAAGCAGGA AAGGATAAAG 
TATATCAAAT TGAAAAAGTC TACGGTTGTT GTACAAGCCT TGGTGCGTGG CTGGTTAGTA 
AGGAAAAGGG TTTCAGAGCA GAAAGCCAAA ACTCGGCTTT TCCACTTTAC AGCAGCTGCC 
TACTGTCACA TGTGTGCTCT TAAAATCCAA CGAGCTTATC GGCTCCACGT GACCTTGAGG 
AACGCTAAGA AGCACATGGA TTCAGTCATC TTCATTCAGA GATGGTTTCG AAAAAGATTA 
CAACGAAAGA GGTTCATTGA ACAGTACCAC AAGATCCTAA GCACTCGACG TGAAGCTCAT 
GCATGTTGGC TCCAGCAGGA CAGGGCTGCA TCCGTGATAC AGAAGGCGGT ACGCCGCTTT 
CTCCTCTGCA GAAGACAGGA AAAGATCACT AGCTGCGCCA CTAGAATTCA GGCATTATGG 
AGAGGCTATT CTTGGAGGAA GAAAAATGAC CACACAGAAA TCAAAGCTAT CCGACGAAGC 
CTTAGAGCTG TGAGCACGAC CGTGGAGGAG GAGAACAAGC TGTACAGAAG AACTGAGCGA 
GCGCTTCATC ACCTGCTCAC CTACAAACAC TTGTCGGCCA TTCTGGATGC GTTGAAGCAC 
CTGGAGGTCG TTACCAGACT CTCTCCACTT TGCTGTGAGA ATATGGCTGA AAGCGGAGCG 
GTATCTACCA TCTTTGTTGT GATCCGAAGC TGTAATCGGA GTGTTCCTTG TATGGAAGTC 
GTTGGATATG CTGTTCAAGT ATTGCTGAAT GTGGCAAAGT ATGATAAGAC GATCGCGGCT 
GTGTACGAGG CCGAGAACTG CGTGGACACA CTGCTGGAGC TTCTGCAGGT GTACCGAGAG 
AAGCCTGGAG ACCGAGTCGC TGAGAAGAGT GCCAGCATTT TCACAAGAAC TTGCTGTTTG 
TTAGCAGTGC TGTTGAAGAC AGAGCAGTGT GCTTTTGATG CACAAAGTAG ATCTAAAGTC 
ACTGATCGTA TTTATCGTCT CTACAAATTT ACAGTTCCTA AGCATAAAGT AAATACACAG 
GGATTGTTTG ATAAGCAGAA GCAGAATTCC TGTGTAGGCT TTCCTTGTAT ACCAGAAAGA 
ACTATGAAGA CCAGGTTAGT TTCAAGACTT AAGCCACAGT GGGTTTTGAG GCGTGATAAT 
GTGGAAGAAA TTACAAACTC CCTGCAAGCT ATTCAACTGG TAATGGATAC ACTTGGAATT 
TCTTATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Longitudinal Diffusion Tensor Imaging Revealed Nerve Fiber Alterations in Aspm Mutated Microcephaly Model Mice.
Neuroscience371325-336(2017 Dec)
Ogi H,Nitta N,Tando S,Fujimori A,Aoki I,Fushiki S,Itoh K

Disruptions in asymmetric centrosome inheritance and WDR62-Aurora kinase B interactions in primary microcephaly.
Scientific reports743708(2017 Mar)
Sgourdou P,Mishra-Gorur K,Saotome I,Henagariu O,Tuysuz B,Campos C,Ishigame K,Giannikou K,Quon JL,Sestan N,Caglayan AO,Gunel M,Louvi A

Embryonic forebrain transcriptome of mice with polyalanine expansion mutations in the ARX homeobox gene.
Human molecular genetics25(24)5433-5443(2016 12)
Mattiske T,Lee K,Gecz J,Friocourt G,Shoubridge C

Microcephaly Proteins Wdr62 and Aspm Define a Mother Centriole Complex Regulating Centriole Biogenesis, Apical Complex, and Cell Fate.
Neuron92(4)813-828(2016 Nov)
Jayaraman D,Kodani A,Gonzalez DM,Mancias JD,Mochida GH,Vagnoni C,Johnson J,Krogan N,Harper JW,Reiter JF,Yu TW,Bae BI,Walsh CA

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Aspm cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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