Cacna1e cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Cacna1e
Entrez Gene ID	12290
Full Name	calcium channel, voltage-dependent, R type, alpha 1E subunit
Synonyms	A430040I15,BII,Cach6,Cacnl1a6,Cav2.3,Cchra1,alpha1E
General protein information	Preferred Names calcium channel, voltage-dependent, R type, alpha 1E subunit Names voltage-dependent R-type calcium channel subunit alpha-1E brain calcium channel II calcium channel, L type, alpha-1 polypeptide calcium channel, voltage-dependent, L type, alpha 1E subunit voltage-gated calcium channel subunit alpha Cav2.3
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 1 Map Location: 1 G3\|1 66.14 cM
Summary	This gene encodes an integral membrane protein that belongs to the calcium channel alpha-1 subunits family. Voltage-sensitive calcium channels mediate the entry of calcium ions into excitable cells and are also involved in a variety of calcium-dependent processes. Voltage-dependent calcium channels are multi-subunit complexes, comprised of alpha-1, alpha-2, beta and delta subunits in a 1:1:1:1 ratio. The isoform alpha-1E gives rise to R-type calcium currents and belongs to the high-voltage activated group. Calcium channels containing the alpha-1E subunit may be involved in the modulation of neuronal firing patterns, an important component of information processing. [provided by RefSeq, Jul 2008].

mRNA and Protein(s)

mRNA	Protein	Name
XM_017312867.1	XP_017168356.1	voltage-dependent R-type calcium channel subunit alpha-1E isoform X16
XM_017312795.1	XP_017168284.1	voltage-dependent R-type calcium channel subunit alpha-1E isoform X12
XM_017312824.1	XP_017168313.1	voltage-dependent R-type calcium channel subunit alpha-1E isoform X13
XM_011247916.3	XP_011246218.1	voltage-dependent R-type calcium channel subunit alpha-1E isoform X14
XM_030244154.1	XP_030100014.1	voltage-dependent R-type calcium channel subunit alpha-1E isoform X15
XM_006529105.4	XP_006529168.1	voltage-dependent R-type calcium channel subunit alpha-1E isoform X18
XM_017312578.1	XP_017168067.1	voltage-dependent R-type calcium channel subunit alpha-1E isoform X1
XM_011247916.2	XP_011246218.1	voltage-dependent R-type calcium channel subunit alpha-1E isoform X14
XM_030243926.1	XP_030099786.1	voltage-dependent R-type calcium channel subunit alpha-1E isoform X1
XM_017312613.1	XP_017168102.1	voltage-dependent R-type calcium channel subunit alpha-1E isoform X2
XM_017312848.1	XP_017168337.1	voltage-dependent R-type calcium channel subunit alpha-1E isoform X14
XM_017312855.2	XP_017168344.1	voltage-dependent R-type calcium channel subunit alpha-1E isoform X16
XM_017312867.2	XP_017168356.1	voltage-dependent R-type calcium channel subunit alpha-1E isoform X17
XM_017312855.1	XP_017168344.1	voltage-dependent R-type calcium channel subunit alpha-1E isoform X15
XM_017312628.1	XP_017168117.1	voltage-dependent R-type calcium channel subunit alpha-1E isoform X4
XM_017312682.1	XP_017168171.1	voltage-dependent R-type calcium channel subunit alpha-1E isoform X7
XM_017312766.1	XP_017168255.1	voltage-dependent R-type calcium channel subunit alpha-1E isoform X11
XM_017312594.1	XP_017168083.1	voltage-dependent R-type calcium channel subunit alpha-1E isoform X1
XM_030243929.1	XP_030099789.1	voltage-dependent R-type calcium channel subunit alpha-1E isoform X1
XM_017312619.1	XP_017168108.1	voltage-dependent R-type calcium channel subunit alpha-1E isoform X3
XM_017312642.1	XP_017168131.1	voltage-dependent R-type calcium channel subunit alpha-1E isoform X5
XM_017312660.1	XP_017168149.1	voltage-dependent R-type calcium channel subunit alpha-1E isoform X6
XM_017312730.1	XP_017168219.1	voltage-dependent R-type calcium channel subunit alpha-1E isoform X9
XM_017312745.1	XP_017168234.1	voltage-dependent R-type calcium channel subunit alpha-1E isoform X10
XM_011247909.2	XP_011246211.1	voltage-dependent R-type calcium channel subunit alpha-1E isoform X1
XM_006529105.3	XP_006529168.1	voltage-dependent R-type calcium channel subunit alpha-1E isoform X17
XM_017312578.2	XP_017168067.1	voltage-dependent R-type calcium channel subunit alpha-1E isoform X1
XM_011247909.3	XP_011246211.1	voltage-dependent R-type calcium channel subunit alpha-1E isoform X1
XM_017312712.1	XP_017168201.1	voltage-dependent R-type calcium channel subunit alpha-1E isoform X8
NM_009782.3	NP_033912.2	voltage-dependent R-type calcium channel subunit alpha-1E

Pathways from BioSystems

Homologies

Rattus norvegicus (Norway rat)	Cacna1e	NP_062167.2
Xenopus tropicalis (tropical clawed frog)	cacna1e	XP_002937409.2
Homo sapiens (human)	CACNA1E	NP_001192222.1
Bos taurus (cattle)	CACNA1E	NP_001178052.1
Mus musculus (house mouse)	Cacna1e	NP_033912.2
Gallus gallus (chicken)	CACNA1E	XP_422255.4
Pan troglodytes (chimpanzee)	CACNA1E	XP_003949701.1
	CACNA1E	XP_001110352.2
Canis lupus familiaris (dog)	CACNA1E	XP_547425.4
Danio rerio (zebrafish)	LOC564274	XP_001920777.3

Gene Ontology
Bibliography

Related articles in PubMed

Unconjugated bilirubin modulates neuronal signaling only in wild-type mice, but not after ablation of the R-type/Ca
Albanna W, Neumaier F, L?ke JN, Kotliar K, Conzen C, Lindauer U, Hescheler J, Clusmann H, Schneider T, Schubert GA
CNS neuroscience & therapeutics(2017 Dec)

miR-34c-5p functions as pronociceptive microRNA in cancer pain by targeting Cav2.3 containing calcium channels.
Gandla J, Lomada SK, Lu J, Kuner R, Bali KK
Pain158(9)1765-1779(2017 Sep)

Gender specific hippocampal whole genome transcriptome data from mice lacking the Ca
Papazoglou A, Henseler C, Lundt A, Wormuth C, Soos J, Broich K, Ehninger D, Weiergr?ber M
Data in brief1281-86(2017 Jun)

Corticothalamic Projection Neuron Development beyond Subtype Specification: Fog2 and Intersectional Controls Regulate Intraclass Neuronal Diversity.
Galazo MJ, Emsley JG, Macklis JD
Neuron91(1)90-106(2016 Jul)

Presynaptic Excitation via GABAB Receptors in Habenula Cholinergic Neurons Regulates Fear Memory Expression.
Zhang J, Tan L, Ren Y, Liang J, Lin R, Feng Q, Zhou J, Hu F, Ren J, Wei C, Yu T, Zhuang Y, Bettler B, Wang F, Luo M
Cell166(3)716-728(2016 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

under conditions of Zn2+ deficiency, ablation or dysfunction of Cav2.3 channels may lead to severe disturbances in glucose homeostasis.
Title: Diethyldithiocarbamate-mediated zinc ion chelation reveals role of Cav2.3 channels in glucagon secretion.

Data show that apamin boosts evoked excitatory postsynaptic potentials (EPSPs) in CaV2.3 R-Type Ca2+ Channel knockout (CaV2.3-/-) mice.
Title: Apamin Boosting of Synaptic Potentials in CaV2.3 R-Type Ca2+ Channel Null Mice.

Cav2.1-2.3 have unique contributions to the dynamics at the Schaffer collateral synapse that are engaged by the complex patterns of afferent activity seen in vivo
Title: Distinct roles for Cav2.1-2.3 in activity-dependent synaptic dynamics.

Low-voltage activated CaV2.3 R-type Ca(2+) channels in the thalamocortical loop and extra-thalamocortical circuitries substantially regulate rodent sleep architecture and represent a novel potential target for pharmacological treatment of sleep disorders
Title: The CaV2.3 R-type voltage-gated Ca2+ channel in mouse sleep architecture.

Cacna1e expression (and Cacna1h) is essential for paraventricular hypothalamic neuronal activity and preventing hyperphagia.
Title: LMO4 is essential for paraventricular hypothalamic neuronal activity and calcium channel expression to prevent hyperphagia.

The following Cacna1e gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Cacna1e cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu27881	XM_006529105.4 Latest version!	Mus musculus calcium channel, voltage-dependent, R type, alpha 1E subunit (Cacna1e), transcript variant X22, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OMu76950	XM_017312867.2 Latest version!	Mus musculus calcium channel, voltage-dependent, R type, alpha 1E subunit (Cacna1e), transcript variant X21, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OMu67260	XM_011247909.3 Latest version!	Mus musculus calcium channel, voltage-dependent, R type, alpha 1E subunit (Cacna1e), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OMu67260	XM_030243926.1 Latest version!	Mus musculus calcium channel, voltage-dependent, R type, alpha 1E subunit (Cacna1e), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OMu67260	XM_017312578.2 Latest version!	Mus musculus calcium channel, voltage-dependent, R type, alpha 1E subunit (Cacna1e), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OMu67260	XM_030243929.1 Latest version!	Mus musculus calcium channel, voltage-dependent, R type, alpha 1E subunit (Cacna1e), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OMu67267	XM_011247916.3 Latest version!	Mus musculus calcium channel, voltage-dependent, R type, alpha 1E subunit (Cacna1e), transcript variant X17, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OMu76948	XM_017312766.1 Latest version!	Mus musculus calcium channel, voltage-dependent, R type, alpha 1E subunit (Cacna1e), transcript variant X14, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OMu76949	XM_017312855.2 Latest version!	Mus musculus calcium channel, voltage-dependent, R type, alpha 1E subunit (Cacna1e), transcript variant X20, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OMu84991	XM_030244154.1 Latest version!	Mus musculus calcium channel, voltage-dependent, R type, alpha 1E subunit (Cacna1e), transcript variant X19, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OMu13081	NM_009782.3 Latest version!	Mus musculus calcium channel, voltage-dependent, R type, alpha 1E subunit (Cacna1e), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1672.30 $2389.00
OMu27870	XM_017312619.1 Latest version!	Mus musculus calcium channel, voltage-dependent, R type, alpha 1E subunit (Cacna1e), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1301.30 $1859.00
OMu27874	XM_017312682.1 Latest version!	Mus musculus calcium channel, voltage-dependent, R type, alpha 1E subunit (Cacna1e), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1301.30 $1859.00
OMu27876	XM_017312745.1 Latest version!	Mus musculus calcium channel, voltage-dependent, R type, alpha 1E subunit (Cacna1e), transcript variant X13, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1301.30 $1859.00
OMu27877	XM_017312795.1 Latest version!	Mus musculus calcium channel, voltage-dependent, R type, alpha 1E subunit (Cacna1e), transcript variant X15, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1301.30 $1859.00
OMu27878	XM_017312824.1 Latest version!	Mus musculus calcium channel, voltage-dependent, R type, alpha 1E subunit (Cacna1e), transcript variant X16, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1301.30 $1859.00
OMu67260	XM_017312594.1 Latest version!	Mus musculus calcium channel, voltage-dependent, R type, alpha 1E subunit (Cacna1e), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1301.30 $1859.00
OMu67261	XM_017312613.1 Latest version!	Mus musculus calcium channel, voltage-dependent, R type, alpha 1E subunit (Cacna1e), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1301.30 $1859.00
OMu67262	XM_017312628.1 Latest version!	Mus musculus calcium channel, voltage-dependent, R type, alpha 1E subunit (Cacna1e), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1301.30 $1859.00
OMu67263	XM_017312642.1 Latest version!	Mus musculus calcium channel, voltage-dependent, R type, alpha 1E subunit (Cacna1e), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1301.30 $1859.00
OMu67264	XM_017312660.1 Latest version!	Mus musculus calcium channel, voltage-dependent, R type, alpha 1E subunit (Cacna1e), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1301.30 $1859.00
OMu67265	XM_017312712.1 Latest version!	Mus musculus calcium channel, voltage-dependent, R type, alpha 1E subunit (Cacna1e), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1301.30 $1859.00
OMu67266	XM_017312730.1 Latest version!	Mus musculus calcium channel, voltage-dependent, R type, alpha 1E subunit (Cacna1e), transcript variant X12, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1301.30 $1859.00
OMu67267	XM_017312848.1 Latest version!	Mus musculus calcium channel, voltage-dependent, R type, alpha 1E subunit (Cacna1e), transcript variant X18, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1091.30 $1559.00
View Old Accession Versions >>
OMu67267	XM_011247916.2	Mus musculus calcium channel, voltage-dependent, R type, alpha 1E subunit (Cacna1e), transcript variant X16, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1091.30 $1559.00
OMu76950	XM_017312867.1	Mus musculus calcium channel, voltage-dependent, R type, alpha 1E subunit (Cacna1e), transcript variant X19, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OMu76949	XM_017312855.1	Mus musculus calcium channel, voltage-dependent, R type, alpha 1E subunit (Cacna1e), transcript variant X18, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OMu27881	XM_006529105.3	Mus musculus calcium channel, voltage-dependent, R type, alpha 1E subunit (Cacna1e), transcript variant X20, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
OMu67260	XM_017312578.1	Mus musculus calcium channel, voltage-dependent, R type, alpha 1E subunit (Cacna1e), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1301.30 $1859.00
OMu67260	XM_011247909.2	Mus musculus calcium channel, voltage-dependent, R type, alpha 1E subunit (Cacna1e), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1301.30 $1859.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Cacna1e ( XM_006529105.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006529168.1 Mus musculus calcium channel, voltage-dependent, R type, alpha 1E subunit (Cacna1e), transcript variant X22, mRNA.

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CloneID	OMu27881
Clone ID Related Accession (Same CDS sequence)	XM_006529105.4 , XM_006529105.3
Accession Version	XM_006529105.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5715bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	voltage-dependent R-type calcium channel subunit alpha-1E isoform X18
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006529105.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGCCCG AGATGCGTGG ACTGAGCCCA GGCCGAAGGC TGCCCGAGCC CACCCTGTGC 
CCGGCTACAG GAGGGAGCCC TGAGCCCGGG GCTGCTGGCA GGCGCCGCCA CGTCCTGGCT 
CTGCCTGATG AGGAGGCATG GCGGCCGCAG CAGTGCGCCC TGCTCGAGGC CAATGCCTCC 
GACGAGGTGG GCATGTTGCC TGCTTCCCCG CGCTCCGCGG TGGCCGGCTT CGACAACTTC 
TTCCCGGTGC AGGAAGGCGA GGGCCCGGGC TGGGAGGGCA GCACCACGCT GGATGCAGGC 
TCCAGCCCCT TCCTTCCTGT GAGCCCAGAG GTCATGAAGC GGAGGCGAGG GGGGCTCATC 
GAGCAGCGGG ACATCATCAA GGCCCACGAG GCACACAAGA TGCAGAGCAC CCCGCAGGCC 
CGGCGCAAGG AGTGGGAGAT GGCTCGCTTC GGGGAGGCGG TGGTCGTTGG CAGGCCAGGC 
TCAGGCGATG GAGACTCGGA CCAGAGCAGG AACCGACAAG GAACCCCCGT CCCGGCCTCG 
GGGCCGGCGG CCGCCTACAA GCAGTCAAAA GCGCAGAGGG CGCGGACTAT GGCTTTGTAC 
AACCCCATAC CAGTCCGGCA GAACTGTTTC ACGGTCAACA GATCCCTGTT CATCTTCGGA 
GAAGATAACA TTGTCAGGAA ATACGCCAAG AAGCTCATCG ATTGGCCGCC ATTTGAGTAC 
ATGATCCTGG CCACCATCAT TGCCAACTGC ATCGTCTTGG CCCTGGAGCA GCATCTTCCT 
GAGGATGACA AGACCCCAAT GTCTCGAAGA CTGGAGAAGA CAGAACCATA TTTCATTGGG 
ATCTTCTGCT TTGAAGCTGG GATCAAAATT GTGGCTCTAG GGTTCATCTT CCATAAGGGT 
TCATACCTCC GTAATGGCTG GAATGTCATG GACTTCATCG TGGTCCTTAG TGGCATCCTG 
GCCACTGCGG GAACCCACTT CAACACCCAC GTGGACCTGC GGACCCTCCG GGCTGTGCGT 
GTCCTGAGGC CTTTAAAGCT GGTATCAGGA ATACCTAGCC TGCAGATCGT GCTGAAGTCT 
ATCATGAAGG CCATGGTGCC TCTTCTGCAG ATTGGCCTTC TGCTCTTCTT TGCCATCCTC 
ATGTTTGCTA TCATTGGCTT GGAGTTCTAC AGTGGCAAGT TGCATCGAGC ATGCTTCATG 
AACAATTCAG GTATTCTAGA GGGGTTTGAT CCTCCTCACC CGTGTGGTGT GCAAGGCTGC 
CCGGCTGGTT ATGAATGTAA GGACTGGATC GGCCCCAATG ACGGGATCAC CCAGTTTGAC 
AACATCCTTT TTGCCGTGCT GACTGTCTTC CAATGCATCA CTATGGAAGG GTGGACCACT 
GTGCTGTACA ATACCAATGA TGCCTTAGGA GCCACCTGGA ACTGGCTGTA CTTCATCCCT 
CTCATCATCA TCGGATCCTT CTTTGTTCTC AACCTCGTCC TGGGAGTGCT TTCCGGGGAA 
TTTGCCAAAG AAAGAGAGAG AGTGGAGAAC CGAAGAGCGT TCATGAAGCT CCGGCGCCAG 
CAACAGATTG AGCGAGAATT GAACGGCTAC CGTGCCTGGA TAGACAAAGC AGAGGAAGTC 
ATGCTTGCTG AAGAAAATAA AAACTCGGGA ACATCAGCCT TGGAAGTGCT TCGAAGGGCA 
ACCATCAAAA GGAGCCGGAC GGAGGCCATG ACCCGAGACT CCAGCGATGA GCACTGCGTT 
GATATCTCTT CAGTGGGCAC ACCTCTTGCC AGAGCCAGTA TCAAGAGCAC AAAAGTAGAT 
GGGGCCTCCT ATTTCCGGCA CAAGGAACGG CTTCTGCGCA TCTCTATCCG CCACATGGTC 
AAGTCCCAAG TCTTTTACTG GATCGTCCTG AGTGTTGTGG CTCTTAATAC TGCCTGTGTG 
GCCATTGTTC ATCACAACCA GCCACAGTGG CTCACTCACC TCCTCTACTA TGCAGAGTTT 
TTGTTTCTGG GACTCTTCCT GTTGGAGATG TCCCTGAAGA TGTATGGCAT GGGGCCACGC 
CTCTATTTTC ACTCATCCTT CAACTGCTTT GATTTTGGGG TCACAGTGGG CAGCATCTTT 
GAGGTTGTCT GGGCAATCTT CAGACCTGGC ACATCTTTTG GAATCAGTGT CTTACGAGCT 
CTCCGGCTCC TAAGGATATT TAAAATAACC AAGTATTGGG CATCCCTCCG GAATCTGGTT 
GTCTCCTTGA TGAGCTCCAT GAAGTCTATC ATCAGCTTAC TCTTCCTTCT CTTCCTCTTC 
ATCGTTGTCT TTGCTCTCCT GGGAATGCAG CTCTTTGGAG GCAGGTTTAA CTTTAATGAT 
GGGACTCCTT CAGCTAATTT TGACACCTTC CCTGCAGCTA TCATGACAGT GTTCCAGATC 
CTGACAGGGG AAGACTGGAA TGAGGTGATG TACAATGGCA TTCGCTCCCA GGGTGGGGTC 
AGCTCAGGCA TGTGGTCTGC CATCTACTTC ATTGTGCTCA CCTTGTTTGG AAACTACACG 
CTGCTAAATG TATTCTTGGC CATCGCTGTG GACAATCTGG CCAATGCCCA GGAACTGACC 
AAGGATGAGC AGGAGGAAGA AGAAGCCTTC AACCAGAAAC ATGCACTGCA GAAGGCCAAG 
GAGGTCAGCC CGATGTCTGC TCCCAACATG CCTTCCATTG AAAGAGACAG AAGGAGAAGA 
CACCACATGT CGATGTGGGA GCCACGCAGC AGCCACCTGA GGGAGCGGAG GCGCCGGCAC 
CACATGTCTG TGTGGGAACA ACGCACCAGC CAGCTGAGGA GGCACATGCA AATGTCTAGC 
CAAGAAGCCC TCAACAAAGA GGAGGCCCCG CCAATGAACC CCCTCAATCC ACTCAACCCA 
CTGAGTCCTC TCAACCCCCT CAATGCTCAC CCCAGCCTTT ATCGGAGGCC CAGGCCCATT 
GAGGGCCTAG CCCTAGGTCT GGGTCTTGAA AAGTGTGAAG AAGAACGCAT CAGTCGTGGG 
GGCTCCCTCA AGGGGGACAT AGGGGGCCTG ACTAGCGCCC TGGACAACCA GAGGAGCCCC 
TTGTCCTTGG GCAAACGGGA GCCACCGTGG CTGCCTAGAT CCTGTCATGG AAACTGTGAC 
CCAATCCAGC AGGAGGCTGG GGGAGGAGAG ACTGTGGTGA CTTTTGAGGA CCGGGCCAGG 
CACAGGCAGA GTCAGAGGCG AAGCCGGCAT CGCCGAGTCA GGACTGAGGG CAAGGACTCT 
GCCTCTGCAT CCCGAAGCAG GTCTGCCAGC CAGGAGCGGA GTCTGGATGA AGGGGTGTCC 
GTTGAGGGGG AGAAGGAACA TGAGCCTCAC AGCAGCCACA GGAGCAAAGA GCCGACCATC 
CACGAAGAGG AGAGAACCCA GGACTTAAGG AGGACCAACA GTCTGATGGT CCCCAGGGGC 
TCTGGACTGG TAGGGGCTCT TGATGAAGCA GAAACACCTC TAGTCCAGCC CCAACCTGAG 
CTGGAAGTGG GGAAGGATGC AGCTCTGACA GAGCAGGAGG CTGAAGGCAG CAGTGAACAA 
GCCCTTCTTG GGGACGTACA GCTGGATGTG GGCCGGGGCA TTAGCCAGAG TGAACCGGAT 
CTCTCCTGCA TGACAGCCAA CATGGACAAG GCTACCACGG AGAGCACCAG TGTCACGGTC 
GCCATCCCTG ATGTGGACCC CTTGGTGGAC TCGACAGTGG TGAACATTAG CAACAAGACG 
GATGGAGAAG CCAGTCCCTT GAAGGAGGCA GAGACTAAAG AGGAAGAAGA AGAGGTAGAG 
AAGAAGAAGA AGCAGAAGAA GGAGAAGCGA GAGACTGGAA AAGCCATGGT GCCCCATAGC 
TCTATGTTCA TCTTCAGCAC CACTAACCCG ATCCGCAGGG CCTGCCACTA CATTGTGAAC 
CTACGCTACT TTGAAATGTG CATTCTCTTG GTGATTGCGG CCAGCAGCAT TGCCCTGGCA 
GCCGAGGACC CTGTACTAAC TAACTCGGAG CGTAACAAAG TCCTGAGATA TTTCGACTAT 
GTTTTCACAG GAGTGTTTAC CTTTGAGATG GTTATAAAGA TGATAGACCA AGGCTTGATC 
CTGCAGGATG GATCATACTT CAGAGACCTC TGGAACATCC TGGACTTTGT AGTAGTGGTT 
GGTGCATTGG TAGCATTTGC TCTGGCGAAC GCTTTGGGAA CCAACAAGGG GAGGGATATC 
AAGACTATCA AGTCTCTGAG GGTACTTCGA GTTCTGCGGC CACTGAAAAC CATCAAGCGC 
TTGCCCAAGC TGAAGGCTGT GTTTGACTGC GTGGTGACCT CCTTGAAGAA TGTCTTCAAC 
ATACTCATTG TCTACAAGCT CTTCATGTTC ATCTTTGCCG TCATCGCTGT TCAGCTATTC 
AAAGGAAAGT TCTTTTATTG CACTGACAGT TCCAAGGACA CAGAGAAGGA GTGCATAGGC 
AACTATGTAG ACCACGAGAA AAACAAGATG GAGGTGAAGG GCCGGGAATG GAAGCGTCAT 
GAATTCCACT ATGACAACAT CATCTGGGCC CTGCTTACCC TCTTCACAGT CTCTACAGGG 
GAAGGATGGC CTCAGGTTCT GCAGCACTCT GTAGATGTGA CAGAGGAGGA CAGAGGCCCA 
AGTCGCAGCA ACCGCATGGA GATGTCCATC TTTTATGTGG TCTACTTTGT GGTCTTCCCT 
TTCTTCTTTG TCAATATCTT CGTGGCTCTC ATTATCATCA CCTTCCAGGA GCAAGGGGAC 
AAGATGATGG AGGAGTGTAG CCTGGAGAAG AATGAGCGGG CATGCATCGA CTTCGCCATT 
AGTGCCAAAC CTCTCACCCG CTACATGCCA CAGAACAGGC ACACCTTCCA GTACCGTGTG 
TGGCACTTTG TAGTCTCCCC ATCCTTTGAG TATACCATCA TGGCCATGAT TGCCTTGAAC 
ACTGTCGTGC TGATGATGAA GTACTACTCT GCTCCTTGCA CCTATGAGCT CGCCTTAAAG 
TACCTGAACA TCGCCTTCAC TATGGTGTTT TCCCTGGAGT GTGTCCTAAA GGTCATTGCT 
TTCGGCTTCC TGAACTATTT CCGAGATACC TGGAACATCT TTGACTTCAT CACGGTGATC 
GGCAGTATCA CAGAAATTAT CCTGACAGAT AGCAAGCTGG TGAACACCAG TGGCTTCAAT 
ATGAGCTTTC TGAAGCTCTT CCGAGCTGCA CGGCTCATAA AGCTTCTGAG ACAGGGCTAC 
ACCATCCGCA TCTTACTGTG GACCTTCGTG CAGTCCTTTA AGGCTCTTCC CTACGTCTGC 
CTTCTCATTG CCATGCTTTT CTTCATCTAC GCCATCATTG GGATGCAGGT GTTTGGAAAC 
ATAAAGTTAG ATGAGGAGAG TCACATCAAC CGGCACAACA ACTTCCGGAG TTTCTTTGGA 
TCCCTCATGC TGCTTTTCAG GAGTGCCACG GGGGAGGCTT GGCAGGAGAT TATGCTGTCC 
TGTCTCGGTG AGAAGGGTTG TGAGCCTGAC ACCACTGCAC CCTCAGGGCA GAATGAGAGT 
GAGCGCTGTG GCACTGACCT GGCCTACGTT TATTTTGTCT CCTTCATCTT CTTCTGCTCC 
TTCCTGATGC TCAACCTGTT CGTGGCTGTC ATCATGGACA ACTTTGAGTA CCTGACTCGA 
GATTCCTCCA TCCTGGGGCC TCACCACTTA GATGAGTTTG TCCGTGTCTG GGCAGAATAT 
GACAGAGCAG CATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006529168.1
CDS	696..6410
Translation	MVPEMRGLSPGRRLPEPTLCPATGGSPEPGAAGRRRHVLALPDEEAWRPQQCALLEANASDEVGMLPASPRSAVAGFDNFFPVQEGEGPGWEGSTTLDAGSSPFLPVSPEVMKRRRGGLIEQRDIIKAHEAHKMQSTPQARRKEWEMARFGEAVVVGRPGSGDGDSDQSRNRQGTPVPASGPAAAYKQSKAQRARTMALYNPIPVRQNCFTVNRSLFIFGEDNIVRKYAKKLIDWPPFEYMILATIIANCIVLALEQHLPEDDKTPMSRRLEKTEPYFIGIFCFEAGIKIVALGFIFHKGSYLRNGWNVMDFIVVLSGILATAGTHFNTHVDLRTLRAVRVLRPLKLVSGIPSLQIVLKSIMKAMVPLLQIGLLLFFAILMFAIIGLEFYSGKLHRACFMNNSGILEGFDPPHPCGVQGCPAGYECKDWIGPNDGITQFDNILFAVLTVFQCITMEGWTTVLYNTNDALGATWNWLYFIPLIIIGSFFVLNLVLGVLSGEFAKERERVENRRAFMKLRRQQQIERELNGYRAWIDKAEEVMLAEENKNSGTSALEVLRRATIKRSRTEAMTRDSSDEHCVDISSVGTPLARASIKSTKVDGASYFRHKERLLRISIRHMVKSQVFYWIVLSVVALNTACVAIVHHNQPQWLTHLLYYAEFLFLGLFLLEMSLKMYGMGPRLYFHSSFNCFDFGVTVGSIFEVVWAIFRPGTSFGISVLRALRLLRIFKITKYWASLRNLVVSLMSSMKSIISLLFLLFLFIVVFALLGMQLFGGRFNFNDGTPSANFDTFPAAIMTVFQILTGEDWNEVMYNGIRSQGGVSSGMWSAIYFIVLTLFGNYTLLNVFLAIAVDNLANAQELTKDEQEEEEAFNQKHALQKAKEVSPMSAPNMPSIERDRRRRHHMSMWEPRSSHLRERRRRHHMSVWEQRTSQLRRHMQMSSQEALNKEEAPPMNPLNPLNPLSPLNPLNAHPSLYRRPRPIEGLALGLGLEKCEEERISRGGSLKGDIGGLTSALDNQRSPLSLGKREPPWLPRSCHGNCDPIQQEAGGGETVVTFEDRARHRQSQRRSRHRRVRTEGKDSASASRSRSASQERSLDEGVSVEGEKEHEPHSSHRSKEPTIHEEERTQDLRRTNSLMVPRGSGLVGALDEAETPLVQPQPELEVGKDAALTEQEAEGSSEQALLGDVQLDVGRGISQSEPDLSCMTANMDKATTESTSVTVAIPDVDPLVDSTVVNISNKTDGEASPLKEAETKEEEEEVEKKKKQKKEKRETGKAMVPHSSMFIFSTTNPIRRACHYIVNLRYFEMCILLVIAASSIALAAEDPVLTNSERNKVLRYFDYVFTGVFTFEMVIKMIDQGLILQDGSYFRDLWNILDFVVVVGALVAFALANALGTNKGRDIKTIKSLRVLRVLRPLKTIKRLPKLKAVFDCVVTSLKNVFNILIVYKLFMFIFAVIAVQLFKGKFFYCTDSSKDTEKECIGNYVDHEKNKMEVKGREWKRHEFHYDNIIWALLTLFTVSTGEGWPQVLQHSVDVTEEDRGPSRSNRMEMSIFYVVYFVVFPFFFVNIFVALIIITFQEQGDKMMEECSLEKNERACIDFAISAKPLTRYMPQNRHTFQYRVWHFVVSPSFEYTIMAMIALNTVVLMMKYYSAPCTYELALKYLNIAFTMVFSLECVLKVIAFGFLNYFRDTWNIFDFITVIGSITEIILTDSKLVNTSGFNMSFLKLFRAARLIKLLRQGYTIRILLWTFVQSFKALPYVCLLIAMLFFIYAIIGMQVFGNIKLDEESHINRHNNFRSFFGSLMLLFRSATGEAWQEIMLSCLGEKGCEPDTTAPSGQNESERCGTDLAYVYFVSFIFFCSFLMLNLFVAVIMDNFEYLTRDSSILGPHHLDEFVRVWAEYDRAA

Target ORF information:

RefSeq Version	XM_006529105.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus calcium channel, voltage-dependent, R type, alpha 1E subunit (Cacna1e), transcript variant X22, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006529105.4

ORF Insert Sequence:

ATGGTGCCCG AGATGCGTGG ACTGAGCCCA GGCCGAAGGC TGCCCGAGCC CACCCTGTGC 
CCGGCTACAG GAGGGAGCCC TGAGCCCGGG GCTGCTGGCA GGCGCCGCCA CGTCCTGGCT 
CTGCCTGATG AGGAGGCATG GCGGCCGCAG CAGTGCGCCC TGCTCGAGGC CAATGCCTCC 
GACGAGGTGG GCATGTTGCC TGCTTCCCCG CGCTCCGCGG TGGCCGGCTT CGACAACTTC 
TTCCCGGTGC AGGAAGGCGA GGGCCCGGGC TGGGAGGGCA GCACCACGCT GGATGCAGGC 
TCCAGCCCCT TCCTTCCTGT GAGCCCAGAG GTCATGAAGC GGAGGCGAGG GGGGCTCATC 
GAGCAGCGGG ACATCATCAA GGCCCACGAG GCACACAAGA TGCAGAGCAC CCCGCAGGCC 
CGGCGCAAGG AGTGGGAGAT GGCTCGCTTC GGGGAGGCGG TGGTCGTTGG CAGGCCAGGC 
TCAGGCGATG GAGACTCGGA CCAGAGCAGG AACCGACAAG GAACCCCCGT CCCGGCCTCG 
GGGCCGGCGG CCGCCTACAA GCAGTCAAAA GCGCAGAGGG CGCGGACTAT GGCTTTGTAC 
AACCCCATAC CAGTCCGGCA GAACTGTTTC ACGGTCAACA GATCCCTGTT CATCTTCGGA 
GAAGATAACA TTGTCAGGAA ATACGCCAAG AAGCTCATCG ATTGGCCGCC ATTTGAGTAC 
ATGATCCTGG CCACCATCAT TGCCAACTGC ATCGTCTTGG CCCTGGAGCA GCATCTTCCT 
GAGGATGACA AGACCCCAAT GTCTCGAAGA CTGGAGAAGA CAGAACCATA TTTCATTGGG 
ATCTTCTGCT TTGAAGCTGG GATCAAAATT GTGGCTCTAG GGTTCATCTT CCATAAGGGT 
TCATACCTCC GTAATGGCTG GAATGTCATG GACTTCATCG TGGTCCTTAG TGGCATCCTG 
GCCACTGCGG GAACCCACTT CAACACCCAC GTGGACCTGC GGACCCTCCG GGCTGTGCGT 
GTCCTGAGGC CTTTAAAGCT GGTATCAGGA ATACCTAGCC TGCAGATCGT GCTGAAGTCT 
ATCATGAAGG CCATGGTGCC TCTTCTGCAG ATTGGCCTTC TGCTCTTCTT TGCCATCCTC 
ATGTTTGCTA TCATTGGCTT GGAGTTCTAC AGTGGCAAGT TGCATCGAGC ATGCTTCATG 
AACAATTCAG GTATTCTAGA GGGGTTTGAT CCTCCTCACC CGTGTGGTGT GCAAGGCTGC 
CCGGCTGGTT ATGAATGTAA GGACTGGATC GGCCCCAATG ACGGGATCAC CCAGTTTGAC 
AACATCCTTT TTGCCGTGCT GACTGTCTTC CAATGCATCA CTATGGAAGG GTGGACCACT 
GTGCTGTACA ATACCAATGA TGCCTTAGGA GCCACCTGGA ACTGGCTGTA CTTCATCCCT 
CTCATCATCA TCGGATCCTT CTTTGTTCTC AACCTCGTCC TGGGAGTGCT TTCCGGGGAA 
TTTGCCAAAG AAAGAGAGAG AGTGGAGAAC CGAAGAGCGT TCATGAAGCT CCGGCGCCAG 
CAACAGATTG AGCGAGAATT GAACGGCTAC CGTGCCTGGA TAGACAAAGC AGAGGAAGTC 
ATGCTTGCTG AAGAAAATAA AAACTCGGGA ACATCAGCCT TGGAAGTGCT TCGAAGGGCA 
ACCATCAAAA GGAGCCGGAC GGAGGCCATG ACCCGAGACT CCAGCGATGA GCACTGCGTT 
GATATCTCTT CAGTGGGCAC ACCTCTTGCC AGAGCCAGTA TCAAGAGCAC AAAAGTAGAT 
GGGGCCTCCT ATTTCCGGCA CAAGGAACGG CTTCTGCGCA TCTCTATCCG CCACATGGTC 
AAGTCCCAAG TCTTTTACTG GATCGTCCTG AGTGTTGTGG CTCTTAATAC TGCCTGTGTG 
GCCATTGTTC ATCACAACCA GCCACAGTGG CTCACTCACC TCCTCTACTA TGCAGAGTTT 
TTGTTTCTGG GACTCTTCCT GTTGGAGATG TCCCTGAAGA TGTATGGCAT GGGGCCACGC 
CTCTATTTTC ACTCATCCTT CAACTGCTTT GATTTTGGGG TCACAGTGGG CAGCATCTTT 
GAGGTTGTCT GGGCAATCTT CAGACCTGGC ACATCTTTTG GAATCAGTGT CTTACGAGCT 
CTCCGGCTCC TAAGGATATT TAAAATAACC AAGTATTGGG CATCCCTCCG GAATCTGGTT 
GTCTCCTTGA TGAGCTCCAT GAAGTCTATC ATCAGCTTAC TCTTCCTTCT CTTCCTCTTC 
ATCGTTGTCT TTGCTCTCCT GGGAATGCAG CTCTTTGGAG GCAGGTTTAA CTTTAATGAT 
GGGACTCCTT CAGCTAATTT TGACACCTTC CCTGCAGCTA TCATGACAGT GTTCCAGATC 
CTGACAGGGG AAGACTGGAA TGAGGTGATG TACAATGGCA TTCGCTCCCA GGGTGGGGTC 
AGCTCAGGCA TGTGGTCTGC CATCTACTTC ATTGTGCTCA CCTTGTTTGG AAACTACACG 
CTGCTAAATG TATTCTTGGC CATCGCTGTG GACAATCTGG CCAATGCCCA GGAACTGACC 
AAGGATGAGC AGGAGGAAGA AGAAGCCTTC AACCAGAAAC ATGCACTGCA GAAGGCCAAG 
GAGGTCAGCC CGATGTCTGC TCCCAACATG CCTTCCATTG AAAGAGACAG AAGGAGAAGA 
CACCACATGT CGATGTGGGA GCCACGCAGC AGCCACCTGA GGGAGCGGAG GCGCCGGCAC 
CACATGTCTG TGTGGGAACA ACGCACCAGC CAGCTGAGGA GGCACATGCA AATGTCTAGC 
CAAGAAGCCC TCAACAAAGA GGAGGCCCCG CCAATGAACC CCCTCAATCC ACTCAACCCA 
CTGAGTCCTC TCAACCCCCT CAATGCTCAC CCCAGCCTTT ATCGGAGGCC CAGGCCCATT 
GAGGGCCTAG CCCTAGGTCT GGGTCTTGAA AAGTGTGAAG AAGAACGCAT CAGTCGTGGG 
GGCTCCCTCA AGGGGGACAT AGGGGGCCTG ACTAGCGCCC TGGACAACCA GAGGAGCCCC 
TTGTCCTTGG GCAAACGGGA GCCACCGTGG CTGCCTAGAT CCTGTCATGG AAACTGTGAC 
CCAATCCAGC AGGAGGCTGG GGGAGGAGAG ACTGTGGTGA CTTTTGAGGA CCGGGCCAGG 
CACAGGCAGA GTCAGAGGCG AAGCCGGCAT CGCCGAGTCA GGACTGAGGG CAAGGACTCT 
GCCTCTGCAT CCCGAAGCAG GTCTGCCAGC CAGGAGCGGA GTCTGGATGA AGGGGTGTCC 
GTTGAGGGGG AGAAGGAACA TGAGCCTCAC AGCAGCCACA GGAGCAAAGA GCCGACCATC 
CACGAAGAGG AGAGAACCCA GGACTTAAGG AGGACCAACA GTCTGATGGT CCCCAGGGGC 
TCTGGACTGG TAGGGGCTCT TGATGAAGCA GAAACACCTC TAGTCCAGCC CCAACCTGAG 
CTGGAAGTGG GGAAGGATGC AGCTCTGACA GAGCAGGAGG CTGAAGGCAG CAGTGAACAA 
GCCCTTCTTG GGGACGTACA GCTGGATGTG GGCCGGGGCA TTAGCCAGAG TGAACCGGAT 
CTCTCCTGCA TGACAGCCAA CATGGACAAG GCTACCACGG AGAGCACCAG TGTCACGGTC 
GCCATCCCTG ATGTGGACCC CTTGGTGGAC TCGACAGTGG TGAACATTAG CAACAAGACG 
GATGGAGAAG CCAGTCCCTT GAAGGAGGCA GAGACTAAAG AGGAAGAAGA AGAGGTAGAG 
AAGAAGAAGA AGCAGAAGAA GGAGAAGCGA GAGACTGGAA AAGCCATGGT GCCCCATAGC 
TCTATGTTCA TCTTCAGCAC CACTAACCCG ATCCGCAGGG CCTGCCACTA CATTGTGAAC 
CTACGCTACT TTGAAATGTG CATTCTCTTG GTGATTGCGG CCAGCAGCAT TGCCCTGGCA 
GCCGAGGACC CTGTACTAAC TAACTCGGAG CGTAACAAAG TCCTGAGATA TTTCGACTAT 
GTTTTCACAG GAGTGTTTAC CTTTGAGATG GTTATAAAGA TGATAGACCA AGGCTTGATC 
CTGCAGGATG GATCATACTT CAGAGACCTC TGGAACATCC TGGACTTTGT AGTAGTGGTT 
GGTGCATTGG TAGCATTTGC TCTGGCGAAC GCTTTGGGAA CCAACAAGGG GAGGGATATC 
AAGACTATCA AGTCTCTGAG GGTACTTCGA GTTCTGCGGC CACTGAAAAC CATCAAGCGC 
TTGCCCAAGC TGAAGGCTGT GTTTGACTGC GTGGTGACCT CCTTGAAGAA TGTCTTCAAC 
ATACTCATTG TCTACAAGCT CTTCATGTTC ATCTTTGCCG TCATCGCTGT TCAGCTATTC 
AAAGGAAAGT TCTTTTATTG CACTGACAGT TCCAAGGACA CAGAGAAGGA GTGCATAGGC 
AACTATGTAG ACCACGAGAA AAACAAGATG GAGGTGAAGG GCCGGGAATG GAAGCGTCAT 
GAATTCCACT ATGACAACAT CATCTGGGCC CTGCTTACCC TCTTCACAGT CTCTACAGGG 
GAAGGATGGC CTCAGGTTCT GCAGCACTCT GTAGATGTGA CAGAGGAGGA CAGAGGCCCA 
AGTCGCAGCA ACCGCATGGA GATGTCCATC TTTTATGTGG TCTACTTTGT GGTCTTCCCT 
TTCTTCTTTG TCAATATCTT CGTGGCTCTC ATTATCATCA CCTTCCAGGA GCAAGGGGAC 
AAGATGATGG AGGAGTGTAG CCTGGAGAAG AATGAGCGGG CATGCATCGA CTTCGCCATT 
AGTGCCAAAC CTCTCACCCG CTACATGCCA CAGAACAGGC ACACCTTCCA GTACCGTGTG 
TGGCACTTTG TAGTCTCCCC ATCCTTTGAG TATACCATCA TGGCCATGAT TGCCTTGAAC 
ACTGTCGTGC TGATGATGAA GTACTACTCT GCTCCTTGCA CCTATGAGCT CGCCTTAAAG 
TACCTGAACA TCGCCTTCAC TATGGTGTTT TCCCTGGAGT GTGTCCTAAA GGTCATTGCT 
TTCGGCTTCC TGAACTATTT CCGAGATACC TGGAACATCT TTGACTTCAT CACGGTGATC 
GGCAGTATCA CAGAAATTAT CCTGACAGAT AGCAAGCTGG TGAACACCAG TGGCTTCAAT 
ATGAGCTTTC TGAAGCTCTT CCGAGCTGCA CGGCTCATAA AGCTTCTGAG ACAGGGCTAC 
ACCATCCGCA TCTTACTGTG GACCTTCGTG CAGTCCTTTA AGGCTCTTCC CTACGTCTGC 
CTTCTCATTG CCATGCTTTT CTTCATCTAC GCCATCATTG GGATGCAGGT GTTTGGAAAC 
ATAAAGTTAG ATGAGGAGAG TCACATCAAC CGGCACAACA ACTTCCGGAG TTTCTTTGGA 
TCCCTCATGC TGCTTTTCAG GAGTGCCACG GGGGAGGCTT GGCAGGAGAT TATGCTGTCC 
TGTCTCGGTG AGAAGGGTTG TGAGCCTGAC ACCACTGCAC CCTCAGGGCA GAATGAGAGT 
GAGCGCTGTG GCACTGACCT GGCCTACGTT TATTTTGTCT CCTTCATCTT CTTCTGCTCC 
TTCCTGATGC TCAACCTGTT CGTGGCTGTC ATCATGGACA ACTTTGAGTA CCTGACTCGA 
GATTCCTCCA TCCTGGGGCC TCACCACTTA GATGAGTTTG TCCGTGTCTG GGCAGAATAT 
GACAGAGCAG CATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cacna1e ( XM_017312867.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017168356.1 Mus musculus calcium channel, voltage-dependent, R type, alpha 1E subunit (Cacna1e), transcript variant X19, mRNA.

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CloneID	OMu76950
Clone ID Related Accession (Same CDS sequence)	XM_017312867.1 , XM_017312867.2
Accession Version	XM_017312867.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5838bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	voltage-dependent R-type calcium channel subunit alpha-1E isoform X16
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGCCCG AGATGCGTGG ACTGAGCCCA GGCCGAAGGC TGCCCGAGCC CACCCTGTGC 
CCGGCTACAG GAGGGAGCCC TGAGCCCGGG GCTGCTGGCA GGCGCCGCCA CGTCCTGGCT 
CTGCCTGATG AGGAGGCATG GCGGCCGCAG CAGTGCGCCC TGCTCGAGGC CAATGCCTCC 
GACGAGGTGG GCATGTTGCC TGCTTCCCCG CGCTCCGCGG TGGCCGGCTT CGACAACTTC 
TTCCCGGTGC AGGAAGGCGA GGGCCCGGGC TGGGAGGGCA GCACCACGCT GGATGCAGGC 
TCCAGCCCCT TCCTTCCTGT GAGCCCAGAG GTCATGAAGC GGAGGCGAGG GGGGCTCATC 
GAGCAGCGGG ACATCATCAA GGCCCACGAG GCACACAAGA TGCAGAGCAC CCCGCAGGCC 
CGGCGCAAGG AGTGGGAGAT GGCTCGCTTC GGGGAGGCGG TGGTCGTTGG CAGGCCAGGC 
TCAGGCGATG GAGACTCGGA CCAGAGCAGG AACCGACAAG GAACCCCCGT CCCGGCCTCG 
GGGCCGGCGG CCGCCTACAA GCAGTCAAAA GCGCAGAGGG CGCGGACTAT GGCTTTGTAC 
AACCCCATAC CAGTCCGGCA GAACTGTTTC ACGGTCAACA GATCCCTGTT CATCTTCGGA 
GAAGATAACA TTGTCAGGAA ATACGCCAAG AAGCTCATCG ATTGGCCGCC ATTTGAGTAC 
ATGATCCTGG CCACCATCAT TGCCAACTGC ATCGTCTTGG CCCTGGAGCA GCATCTTCCT 
GAGGATGACA AGACCCCAAT GTCTCGAAGA CTGGAGAAGA CAGAACCATA TTTCATTGGG 
ATCTTCTGCT TTGAAGCTGG GATCAAAATT GTGGCTCTAG GGTTCATCTT CCATAAGGGT 
TCATACCTCC GTAATGGCTG GAATGTCATG GACTTCATCG TGGTCCTTAG TGGCATCCTG 
GCCACTGCGG GAACCCACTT CAACACCCAC GTGGACCTGC GGACCCTCCG GGCTGTGCGT 
GTCCTGAGGC CTTTAAAGCT GGTATCAGGA ATACCTAGCC TGCAGATCGT GCTGAAGTCT 
ATCATGAAGG CCATGGTGCC TCTTCTGCAG ATTGGCCTTC TGCTCTTCTT TGCCATCCTC 
ATGTTTGCTA TCATTGGCTT GGAGTTCTAC AGTGGCAAGT TGCATCGAGC ATGCTTCATG 
AACAATTCAG GTATTCTAGA GGGGTTTGAT CCTCCTCACC CGTGTGGTGT GCAAGGCTGC 
CCGGCTGGTT ATGAATGTAA GGACTGGATC GGCCCCAATG ACGGGATCAC CCAGTTTGAC 
AACATCCTTT TTGCCGTGCT GACTGTCTTC CAATGCATCA CTATGGAAGG GTGGACCACT 
GTGCTGTACA ATACCAATGA TGCCTTAGGA GCCACCTGGA ACTGGCTGTA CTTCATCCCT 
CTCATCATCA TCGGATCCTT CTTTGTTCTC AACCTCGTCC TGGGAGTGCT TTCCGGGGAA 
TTTGCCAAAG AAAGAGAGAG AGTGGAGAAC CGAAGAGCGT TCATGAAGCT CCGGCGCCAG 
CAACAGATTG AGCGAGAATT GAACGGCTAC CGTGCCTGGA TAGACAAAGC AGAGGAAGTC 
ATGCTTGCTG AAGAAAATAA AAACTCGGGA ACATCAGCCT TGGAAGTGCT TCGAAGGGCA 
ACCATCAAAA GGAGCCGGAC GGAGGCCATG ACCCGAGACT CCAGCGATGA GCACTGCGTT 
GATATCTCTT CAGTGGGCAC ACCTCTTGCC AGAGCCAGTA TCAAGAGCAC AAAAGTAGAT 
GGGGCCTCCT ATTTCCGGCA CAAGGAACGG CTTCTGCGCA TCTCTATCCG CCACATGGTC 
AAGTCCCAAG TCTTTTACTG GATCGTCCTG AGTGTTGTGG CTCTTAATAC TGCCTGTGTG 
GCCATTGTTC ATCACAACCA GCCACAGTGG CTCACTCACC TCCTCTACTA TGCAGAGTTT 
TTGTTTCTGG GACTCTTCCT GTTGGAGATG TCCCTGAAGA TGTATGGCAT GGGGCCACGC 
CTCTATTTTC ACTCATCCTT CAACTGCTTT GATTTTGGGG TCACAGTGGG CAGCATCTTT 
GAGGTTGTCT GGGCAATCTT CAGACCTGGC ACATCTTTTG GAATCAGTGT CTTACGAGCT 
CTCCGGCTCC TAAGGATATT TAAAATAACC AAGTATTGGG CATCCCTCCG GAATCTGGTT 
GTCTCCTTGA TGAGCTCCAT GAAGTCTATC ATCAGCTTAC TCTTCCTTCT CTTCCTCTTC 
ATCGTTGTCT TTGCTCTCCT GGGAATGCAG CTCTTTGGAG GCAGGTTTAA CTTTAATGAT 
GGGACTCCTT CAGCTAATTT TGACACCTTC CCTGCAGCTA TCATGACAGT GTTCCAGATC 
CTGACAGGGG AAGACTGGAA TGAGGTGATG TACAATGGCA TTCGCTCCCA GGGTGGGGTC 
AGCTCAGGCA TGTGGTCTGC CATCTACTTC ATTGTGCTCA CCTTGTTTGG AAACTACACG 
CTGCTAAATG TATTCTTGGC CATCGCTGTG GACAATCTGG CCAATGCCCA GGAACTGACC 
AAGGATGAGC AGGAGGAAGA AGAAGCCTTC AACCAGAAAC ATGCACTGCA GAAGGCCAAG 
GAGGTCAGCC CGATGTCTGC TCCCAACATG CCTTCCATTG AAAGAGACAG AAGGAGAAGA 
CACCACATGT CGATGTGGGA GCCACGCAGC AGCCACCTGA GGGAGCGGAG GCGCCGGCAC 
CACATGTCTG TGTGGGAACA ACGCACCAGC CAGCTGAGGA GGCACATGCA AATGTCTAGC 
CAAGAAGCCC TCAACAAAGA GGAGGCCCCG CCAATGAACC CCCTCAATCC ACTCAACCCA 
CTGAGTCCTC TCAACCCCCT CAATGCTCAC CCCAGCCTTT ATCGGAGGCC CAGGCCCATT 
GAGGGCCTAG CCCTAGGTCT GGGTCTTGAA AAGTGTGAAG AAGAACGCAT CAGTCGTGGG 
GGCTCCCTCA AGGGGGACAT AGGGGGCCTG ACTAGCGCCC TGGACAACCA GAGGAGCCCC 
TTGTCCTTGG GCAAACGGGA GCCACCGTGG CTGCCTAGAT CCTGTCATGG AAACTGTGAC 
CCAATCCAGC AGGAGGCTGG GGGAGGAGAG ACTGTGGTGA CTTTTGAGGA CCGGGCCAGG 
CACAGGCAGA GTCAGAGGCG AAGCCGGCAT CGCCGAGTCA GGACTGAGGG CAAGGACTCT 
GCCTCTGCAT CCCGAAGCAG GTCTGCCAGC CAGGAGCGGA GTCTGGATGA AGGGGTGTCC 
GTTGAGGGGG AGAAGGAACA TGAGCCTCAC AGCAGCCACA GGAGCAAAGA GCCGACCATC 
CACGAAGAGG AGAGAACCCA GGACTTAAGG AGGACCAACA GTCTGATGGT CCCCAGGGGC 
TCTGGACTGG TAGGGGCTCT TGATGAAGCA GAAACACCTC TAGTCCAGCC CCAACCTGAG 
CTGGAAGTGG GGAAGGATGC AGCTCTGACA GAGCAGGAGG CTGAAGGCAG CAGTGAACAA 
GCCCTTCTTG GGGACGTACA GCTGGATGTG GGCCGGGGCA TTAGCCAGAG TGAACCGGAT 
CTCTCCTGCA TGACAGCCAA CATGGACAAG GCTACCACGG AGAGCACCAG TGTCACGGTC 
GCCATCCCTG ATGTGGACCC CTTGGTGGAC TCGACAGTGG TGAACATTAG CAACAAGACG 
GATGGAGAAG CCAGTCCCTT GAAGGAGGCA GAGACTAAAG AGGAAGAAGA AGAGGTAGAG 
AAGAAGAAGA AGCAGAAGAA GGAGAAGCGA GAGACTGGAA AAGCCATGGT GCCCCATAGC 
TCTATGTTCA TCTTCAGCAC CACTAACCCG ATCCGCAGGG CCTGCCACTA CATTGTGAAC 
CTACGCTACT TTGAAATGTG CATTCTCTTG GTGATTGCGG CCAGCAGCAT TGCCCTGGCA 
GCCGAGGACC CTGTACTAAC TAACTCGGAG CGTAACAAAG TCCTGAGATA TTTCGACTAT 
GTTTTCACAG GAGTGTTTAC CTTTGAGATG GTTATAAAGA TGATAGACCA AGGCTTGATC 
CTGCAGGATG GATCATACTT CAGAGACCTC TGGAACATCC TGGACTTTGT AGTAGTGGTT 
GGTGCATTGG TAGCATTTGC TCTGGCGAAC GCTTTGGGAA CCAACAAGGG GAGGGATATC 
AAGACTATCA AGTCTCTGAG GGTACTTCGA GTTCTGCGGC CACTGAAAAC CATCAAGCGC 
TTGCCCAAGC TGAAGGCTGT GTTTGACTGC GTGGTGACCT CCTTGAAGAA TGTCTTCAAC 
ATACTCATTG TCTACAAGCT CTTCATGTTC ATCTTTGCCG TCATCGCTGT TCAGCTATTC 
AAAGGAAAGT TCTTTTATTG CACTGACAGT TCCAAGGACA CAGAGAAGGA GTGCATAGGC 
AACTATGTAG ACCACGAGAA AAACAAGATG GAGGTGAAGG GCCGGGAATG GAAGCGTCAT 
GAATTCCACT ATGACAACAT CATCTGGGCC CTGCTTACCC TCTTCACAGT CTCTACAGGG 
GAAGGATGGC CTCAGGTTCT GCAGCACTCT GTAGATGTGA CAGAGGAGGA CAGAGGCCCA 
AGTCGCAGCA ACCGCATGGA GATGTCCATC TTTTATGTGG TCTACTTTGT GGTCTTCCCT 
TTCTTCTTTG TCAATATCTT CGTGGCTCTC ATTATCATCA CCTTCCAGGA GCAAGGGGAC 
AAGATGATGG AGGAGTGTAG CCTGGAGAAG AATGAGCGGG CATGCATCGA CTTCGCCATT 
AGTGCCAAAC CTCTCACCCG CTACATGCCA CAGAACAGGC ACACCTTCCA GTACCGTGTG 
TGGCACTTTG TAGTCTCCCC ATCCTTTGAG TATACCATCA TGGCCATGAT TGCCTTGAAC 
ACTGTCGTGC TGATGATGAA GTACTACTCT GCTCCTTGCA CCTATGAGCT CGCCTTAAAG 
TACCTGAACA TCGCCTTCAC TATGGTGTTT TCCCTGGAGT GTGTCCTAAA GGTCATTGCT 
TTCGGCTTCC TGAACTATTT CCGAGATACC TGGAACATCT TTGACTTCAT CACGGTGATC 
GGCAGTATCA CAGAAATTAT CCTGACAGAT AGCAAGCTGG TGAACACCAG TGGCTTCAAT 
ATGAGCTTTC TGAAGCTCTT CCGAGCTGCA CGGCTCATAA AGCTTCTGAG ACAGGGCTAC 
ACCATCCGCA TCTTACTGTG GACCTTCGTG CAGTCCTTTA AGGCTCTTCC CTACGTCTGC 
CTTCTCATTG CCATGCTTTT CTTCATCTAC GCCATCATTG GGATGCAGGT GTTTGGAAAC 
ATAAAGTTAG ATGAGGAGAG TCACATCAAC CGGCACAACA ACTTCCGGAG TTTCTTTGGA 
TCCCTCATGC TGCTTTTCAG GAGTGCCACG GGGGAGGCTT GGCAGGAGAT TATGCTGTCC 
TGTCTCGGTG AGAAGGGTTG TGAGCCTGAC ACCACTGCAC CCTCAGGGCA GAATGAGAGT 
GAGCGCTGTG GCACTGACCT GGCCTACGTT TATTTTGTCT CCTTCATCTT CTTCTGCTCC 
TTCCTGATGC TCAACCTGTT CGTGGCTGTC ATCATGGACA ACTTTGAGTA CCTGACTCGA 
GATTCCTCCA TCCTGGGGCC TCACCACTTA GATGAGTTTG TCCGTGTCTG GGCAGAATAT 
GACAGAGCAG CATGCGGCAG GATCCCGTAT AAGGATATGT ACAAATTAGT GCGGGTGATC 
TCGCCTCCTC TGGGCCTGGG AGAGAACTGC CCCTACAGGG TGGCTTGCAA GGTTTGCCCC 
CCCAGTCCCC AGCCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017168356.1
CDS	698..6535
Translation	MVPEMRGLSPGRRLPEPTLCPATGGSPEPGAAGRRRHVLALPDEEAWRPQQCALLEANASDEVGMLPASPRSAVAGFDNFFPVQEGEGPGWEGSTTLDAGSSPFLPVSPEVMKRRRGGLIEQRDIIKAHEAHKMQSTPQARRKEWEMARFGEAVVVGRPGSGDGDSDQSRNRQGTPVPASGPAAAYKQSKAQRARTMALYNPIPVRQNCFTVNRSLFIFGEDNIVRKYAKKLIDWPPFEYMILATIIANCIVLALEQHLPEDDKTPMSRRLEKTEPYFIGIFCFEAGIKIVALGFIFHKGSYLRNGWNVMDFIVVLSGILATAGTHFNTHVDLRTLRAVRVLRPLKLVSGIPSLQIVLKSIMKAMVPLLQIGLLLFFAILMFAIIGLEFYSGKLHRACFMNNSGILEGFDPPHPCGVQGCPAGYECKDWIGPNDGITQFDNILFAVLTVFQCITMEGWTTVLYNTNDALGATWNWLYFIPLIIIGSFFVLNLVLGVLSGEFAKERERVENRRAFMKLRRQQQIERELNGYRAWIDKAEEVMLAEENKNSGTSALEVLRRATIKRSRTEAMTRDSSDEHCVDISSVGTPLARASIKSTKVDGASYFRHKERLLRISIRHMVKSQVFYWIVLSVVALNTACVAIVHHNQPQWLTHLLYYAEFLFLGLFLLEMSLKMYGMGPRLYFHSSFNCFDFGVTVGSIFEVVWAIFRPGTSFGISVLRALRLLRIFKITKYWASLRNLVVSLMSSMKSIISLLFLLFLFIVVFALLGMQLFGGRFNFNDGTPSANFDTFPAAIMTVFQILTGEDWNEVMYNGIRSQGGVSSGMWSAIYFIVLTLFGNYTLLNVFLAIAVDNLANAQELTKDEQEEEEAFNQKHALQKAKEVSPMSAPNMPSIERDRRRRHHMSMWEPRSSHLRERRRRHHMSVWEQRTSQLRRHMQMSSQEALNKEEAPPMNPLNPLNPLSPLNPLNAHPSLYRRPRPIEGLALGLGLEKCEEERISRGGSLKGDIGGLTSALDNQRSPLSLGKREPPWLPRSCHGNCDPIQQEAGGGETVVTFEDRARHRQSQRRSRHRRVRTEGKDSASASRSRSASQERSLDEGVSVEGEKEHEPHSSHRSKEPTIHEEERTQDLRRTNSLMVPRGSGLVGALDEAETPLVQPQPELEVGKDAALTEQEAEGSSEQALLGDVQLDVGRGISQSEPDLSCMTANMDKATTESTSVTVAIPDVDPLVDSTVVNISNKTDGEASPLKEAETKEEEEEVEKKKKQKKEKRETGKAMVPHSSMFIFSTTNPIRRACHYIVNLRYFEMCILLVIAASSIALAAEDPVLTNSERNKVLRYFDYVFTGVFTFEMVIKMIDQGLILQDGSYFRDLWNILDFVVVVGALVAFALANALGTNKGRDIKTIKSLRVLRVLRPLKTIKRLPKLKAVFDCVVTSLKNVFNILIVYKLFMFIFAVIAVQLFKGKFFYCTDSSKDTEKECIGNYVDHEKNKMEVKGREWKRHEFHYDNIIWALLTLFTVSTGEGWPQVLQHSVDVTEEDRGPSRSNRMEMSIFYVVYFVVFPFFFVNIFVALIIITFQEQGDKMMEECSLEKNERACIDFAISAKPLTRYMPQNRHTFQYRVWHFVVSPSFEYTIMAMIALNTVVLMMKYYSAPCTYELALKYLNIAFTMVFSLECVLKVIAFGFLNYFRDTWNIFDFITVIGSITEIILTDSKLVNTSGFNMSFLKLFRAARLIKLLRQGYTIRILLWTFVQSFKALPYVCLLIAMLFFIYAIIGMQVFGNIKLDEESHINRHNNFRSFFGSLMLLFRSATGEAWQEIMLSCLGEKGCEPDTTAPSGQNESERCGTDLAYVYFVSFIFFCSFLMLNLFVAVIMDNFEYLTRDSSILGPHHLDEFVRVWAEYDRAACGRIPYKDMYKLVRVISPPLGLGENCPYRVACKVCPPSPQP

Target ORF information:

RefSeq Version	XM_017312867.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus calcium channel, voltage-dependent, R type, alpha 1E subunit (Cacna1e), transcript variant X19, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017312867.1

ORF Insert Sequence:

ATGGTGCCCG AGATGCGTGG ACTGAGCCCA GGCCGAAGGC TGCCCGAGCC CACCCTGTGC 
CCGGCTACAG GAGGGAGCCC TGAGCCCGGG GCTGCTGGCA GGCGCCGCCA CGTCCTGGCT 
CTGCCTGATG AGGAGGCATG GCGGCCGCAG CAGTGCGCCC TGCTCGAGGC CAATGCCTCC 
GACGAGGTGG GCATGTTGCC TGCTTCCCCG CGCTCCGCGG TGGCCGGCTT CGACAACTTC 
TTCCCGGTGC AGGAAGGCGA GGGCCCGGGC TGGGAGGGCA GCACCACGCT GGATGCAGGC 
TCCAGCCCCT TCCTTCCTGT GAGCCCAGAG GTCATGAAGC GGAGGCGAGG GGGGCTCATC 
GAGCAGCGGG ACATCATCAA GGCCCACGAG GCACACAAGA TGCAGAGCAC CCCGCAGGCC 
CGGCGCAAGG AGTGGGAGAT GGCTCGCTTC GGGGAGGCGG TGGTCGTTGG CAGGCCAGGC 
TCAGGCGATG GAGACTCGGA CCAGAGCAGG AACCGACAAG GAACCCCCGT CCCGGCCTCG 
GGGCCGGCGG CCGCCTACAA GCAGTCAAAA GCGCAGAGGG CGCGGACTAT GGCTTTGTAC 
AACCCCATAC CAGTCCGGCA GAACTGTTTC ACGGTCAACA GATCCCTGTT CATCTTCGGA 
GAAGATAACA TTGTCAGGAA ATACGCCAAG AAGCTCATCG ATTGGCCGCC ATTTGAGTAC 
ATGATCCTGG CCACCATCAT TGCCAACTGC ATCGTCTTGG CCCTGGAGCA GCATCTTCCT 
GAGGATGACA AGACCCCAAT GTCTCGAAGA CTGGAGAAGA CAGAACCATA TTTCATTGGG 
ATCTTCTGCT TTGAAGCTGG GATCAAAATT GTGGCTCTAG GGTTCATCTT CCATAAGGGT 
TCATACCTCC GTAATGGCTG GAATGTCATG GACTTCATCG TGGTCCTTAG TGGCATCCTG 
GCCACTGCGG GAACCCACTT CAACACCCAC GTGGACCTGC GGACCCTCCG GGCTGTGCGT 
GTCCTGAGGC CTTTAAAGCT GGTATCAGGA ATACCTAGCC TGCAGATCGT GCTGAAGTCT 
ATCATGAAGG CCATGGTGCC TCTTCTGCAG ATTGGCCTTC TGCTCTTCTT TGCCATCCTC 
ATGTTTGCTA TCATTGGCTT GGAGTTCTAC AGTGGCAAGT TGCATCGAGC ATGCTTCATG 
AACAATTCAG GTATTCTAGA GGGGTTTGAT CCTCCTCACC CGTGTGGTGT GCAAGGCTGC 
CCGGCTGGTT ATGAATGTAA GGACTGGATC GGCCCCAATG ACGGGATCAC CCAGTTTGAC 
AACATCCTTT TTGCCGTGCT GACTGTCTTC CAATGCATCA CTATGGAAGG GTGGACCACT 
GTGCTGTACA ATACCAATGA TGCCTTAGGA GCCACCTGGA ACTGGCTGTA CTTCATCCCT 
CTCATCATCA TCGGATCCTT CTTTGTTCTC AACCTCGTCC TGGGAGTGCT TTCCGGGGAA 
TTTGCCAAAG AAAGAGAGAG AGTGGAGAAC CGAAGAGCGT TCATGAAGCT CCGGCGCCAG 
CAACAGATTG AGCGAGAATT GAACGGCTAC CGTGCCTGGA TAGACAAAGC AGAGGAAGTC 
ATGCTTGCTG AAGAAAATAA AAACTCGGGA ACATCAGCCT TGGAAGTGCT TCGAAGGGCA 
ACCATCAAAA GGAGCCGGAC GGAGGCCATG ACCCGAGACT CCAGCGATGA GCACTGCGTT 
GATATCTCTT CAGTGGGCAC ACCTCTTGCC AGAGCCAGTA TCAAGAGCAC AAAAGTAGAT 
GGGGCCTCCT ATTTCCGGCA CAAGGAACGG CTTCTGCGCA TCTCTATCCG CCACATGGTC 
AAGTCCCAAG TCTTTTACTG GATCGTCCTG AGTGTTGTGG CTCTTAATAC TGCCTGTGTG 
GCCATTGTTC ATCACAACCA GCCACAGTGG CTCACTCACC TCCTCTACTA TGCAGAGTTT 
TTGTTTCTGG GACTCTTCCT GTTGGAGATG TCCCTGAAGA TGTATGGCAT GGGGCCACGC 
CTCTATTTTC ACTCATCCTT CAACTGCTTT GATTTTGGGG TCACAGTGGG CAGCATCTTT 
GAGGTTGTCT GGGCAATCTT CAGACCTGGC ACATCTTTTG GAATCAGTGT CTTACGAGCT 
CTCCGGCTCC TAAGGATATT TAAAATAACC AAGTATTGGG CATCCCTCCG GAATCTGGTT 
GTCTCCTTGA TGAGCTCCAT GAAGTCTATC ATCAGCTTAC TCTTCCTTCT CTTCCTCTTC 
ATCGTTGTCT TTGCTCTCCT GGGAATGCAG CTCTTTGGAG GCAGGTTTAA CTTTAATGAT 
GGGACTCCTT CAGCTAATTT TGACACCTTC CCTGCAGCTA TCATGACAGT GTTCCAGATC 
CTGACAGGGG AAGACTGGAA TGAGGTGATG TACAATGGCA TTCGCTCCCA GGGTGGGGTC 
AGCTCAGGCA TGTGGTCTGC CATCTACTTC ATTGTGCTCA CCTTGTTTGG AAACTACACG 
CTGCTAAATG TATTCTTGGC CATCGCTGTG GACAATCTGG CCAATGCCCA GGAACTGACC 
AAGGATGAGC AGGAGGAAGA AGAAGCCTTC AACCAGAAAC ATGCACTGCA GAAGGCCAAG 
GAGGTCAGCC CGATGTCTGC TCCCAACATG CCTTCCATTG AAAGAGACAG AAGGAGAAGA 
CACCACATGT CGATGTGGGA GCCACGCAGC AGCCACCTGA GGGAGCGGAG GCGCCGGCAC 
CACATGTCTG TGTGGGAACA ACGCACCAGC CAGCTGAGGA GGCACATGCA AATGTCTAGC 
CAAGAAGCCC TCAACAAAGA GGAGGCCCCG CCAATGAACC CCCTCAATCC ACTCAACCCA 
CTGAGTCCTC TCAACCCCCT CAATGCTCAC CCCAGCCTTT ATCGGAGGCC CAGGCCCATT 
GAGGGCCTAG CCCTAGGTCT GGGTCTTGAA AAGTGTGAAG AAGAACGCAT CAGTCGTGGG 
GGCTCCCTCA AGGGGGACAT AGGGGGCCTG ACTAGCGCCC TGGACAACCA GAGGAGCCCC 
TTGTCCTTGG GCAAACGGGA GCCACCGTGG CTGCCTAGAT CCTGTCATGG AAACTGTGAC 
CCAATCCAGC AGGAGGCTGG GGGAGGAGAG ACTGTGGTGA CTTTTGAGGA CCGGGCCAGG 
CACAGGCAGA GTCAGAGGCG AAGCCGGCAT CGCCGAGTCA GGACTGAGGG CAAGGACTCT 
GCCTCTGCAT CCCGAAGCAG GTCTGCCAGC CAGGAGCGGA GTCTGGATGA AGGGGTGTCC 
GTTGAGGGGG AGAAGGAACA TGAGCCTCAC AGCAGCCACA GGAGCAAAGA GCCGACCATC 
CACGAAGAGG AGAGAACCCA GGACTTAAGG AGGACCAACA GTCTGATGGT CCCCAGGGGC 
TCTGGACTGG TAGGGGCTCT TGATGAAGCA GAAACACCTC TAGTCCAGCC CCAACCTGAG 
CTGGAAGTGG GGAAGGATGC AGCTCTGACA GAGCAGGAGG CTGAAGGCAG CAGTGAACAA 
GCCCTTCTTG GGGACGTACA GCTGGATGTG GGCCGGGGCA TTAGCCAGAG TGAACCGGAT 
CTCTCCTGCA TGACAGCCAA CATGGACAAG GCTACCACGG AGAGCACCAG TGTCACGGTC 
GCCATCCCTG ATGTGGACCC CTTGGTGGAC TCGACAGTGG TGAACATTAG CAACAAGACG 
GATGGAGAAG CCAGTCCCTT GAAGGAGGCA GAGACTAAAG AGGAAGAAGA AGAGGTAGAG 
AAGAAGAAGA AGCAGAAGAA GGAGAAGCGA GAGACTGGAA AAGCCATGGT GCCCCATAGC 
TCTATGTTCA TCTTCAGCAC CACTAACCCG ATCCGCAGGG CCTGCCACTA CATTGTGAAC 
CTACGCTACT TTGAAATGTG CATTCTCTTG GTGATTGCGG CCAGCAGCAT TGCCCTGGCA 
GCCGAGGACC CTGTACTAAC TAACTCGGAG CGTAACAAAG TCCTGAGATA TTTCGACTAT 
GTTTTCACAG GAGTGTTTAC CTTTGAGATG GTTATAAAGA TGATAGACCA AGGCTTGATC 
CTGCAGGATG GATCATACTT CAGAGACCTC TGGAACATCC TGGACTTTGT AGTAGTGGTT 
GGTGCATTGG TAGCATTTGC TCTGGCGAAC GCTTTGGGAA CCAACAAGGG GAGGGATATC 
AAGACTATCA AGTCTCTGAG GGTACTTCGA GTTCTGCGGC CACTGAAAAC CATCAAGCGC 
TTGCCCAAGC TGAAGGCTGT GTTTGACTGC GTGGTGACCT CCTTGAAGAA TGTCTTCAAC 
ATACTCATTG TCTACAAGCT CTTCATGTTC ATCTTTGCCG TCATCGCTGT TCAGCTATTC 
AAAGGAAAGT TCTTTTATTG CACTGACAGT TCCAAGGACA CAGAGAAGGA GTGCATAGGC 
AACTATGTAG ACCACGAGAA AAACAAGATG GAGGTGAAGG GCCGGGAATG GAAGCGTCAT 
GAATTCCACT ATGACAACAT CATCTGGGCC CTGCTTACCC TCTTCACAGT CTCTACAGGG 
GAAGGATGGC CTCAGGTTCT GCAGCACTCT GTAGATGTGA CAGAGGAGGA CAGAGGCCCA 
AGTCGCAGCA ACCGCATGGA GATGTCCATC TTTTATGTGG TCTACTTTGT GGTCTTCCCT 
TTCTTCTTTG TCAATATCTT CGTGGCTCTC ATTATCATCA CCTTCCAGGA GCAAGGGGAC 
AAGATGATGG AGGAGTGTAG CCTGGAGAAG AATGAGCGGG CATGCATCGA CTTCGCCATT 
AGTGCCAAAC CTCTCACCCG CTACATGCCA CAGAACAGGC ACACCTTCCA GTACCGTGTG 
TGGCACTTTG TAGTCTCCCC ATCCTTTGAG TATACCATCA TGGCCATGAT TGCCTTGAAC 
ACTGTCGTGC TGATGATGAA GTACTACTCT GCTCCTTGCA CCTATGAGCT CGCCTTAAAG 
TACCTGAACA TCGCCTTCAC TATGGTGTTT TCCCTGGAGT GTGTCCTAAA GGTCATTGCT 
TTCGGCTTCC TGAACTATTT CCGAGATACC TGGAACATCT TTGACTTCAT CACGGTGATC 
GGCAGTATCA CAGAAATTAT CCTGACAGAT AGCAAGCTGG TGAACACCAG TGGCTTCAAT 
ATGAGCTTTC TGAAGCTCTT CCGAGCTGCA CGGCTCATAA AGCTTCTGAG ACAGGGCTAC 
ACCATCCGCA TCTTACTGTG GACCTTCGTG CAGTCCTTTA AGGCTCTTCC CTACGTCTGC 
CTTCTCATTG CCATGCTTTT CTTCATCTAC GCCATCATTG GGATGCAGGT GTTTGGAAAC 
ATAAAGTTAG ATGAGGAGAG TCACATCAAC CGGCACAACA ACTTCCGGAG TTTCTTTGGA 
TCCCTCATGC TGCTTTTCAG GAGTGCCACG GGGGAGGCTT GGCAGGAGAT TATGCTGTCC 
TGTCTCGGTG AGAAGGGTTG TGAGCCTGAC ACCACTGCAC CCTCAGGGCA GAATGAGAGT 
GAGCGCTGTG GCACTGACCT GGCCTACGTT TATTTTGTCT CCTTCATCTT CTTCTGCTCC 
TTCCTGATGC TCAACCTGTT CGTGGCTGTC ATCATGGACA ACTTTGAGTA CCTGACTCGA 
GATTCCTCCA TCCTGGGGCC TCACCACTTA GATGAGTTTG TCCGTGTCTG GGCAGAATAT 
GACAGAGCAG CATGCGGCAG GATCCCGTAT AAGGATATGT ACAAATTAGT GCGGGTGATC 
TCGCCTCCTC TGGGCCTGGG AGAGAACTGC CCCTACAGGG TGGCTTGCAA GGTTTGCCCC 
CCCAGTCCCC AGCCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cacna1e ( XM_017312867.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017168356.1 Mus musculus calcium channel, voltage-dependent, R type, alpha 1E subunit (Cacna1e), transcript variant X21, mRNA.

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CloneID	OMu76950
Clone ID Related Accession (Same CDS sequence)	XM_017312867.1 , XM_017312867.2
Accession Version	XM_017312867.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5838bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	voltage-dependent R-type calcium channel subunit alpha-1E isoform X17
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_017312867.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGCCCG AGATGCGTGG ACTGAGCCCA GGCCGAAGGC TGCCCGAGCC CACCCTGTGC 
CCGGCTACAG GAGGGAGCCC TGAGCCCGGG GCTGCTGGCA GGCGCCGCCA CGTCCTGGCT 
CTGCCTGATG AGGAGGCATG GCGGCCGCAG CAGTGCGCCC TGCTCGAGGC CAATGCCTCC 
GACGAGGTGG GCATGTTGCC TGCTTCCCCG CGCTCCGCGG TGGCCGGCTT CGACAACTTC 
TTCCCGGTGC AGGAAGGCGA GGGCCCGGGC TGGGAGGGCA GCACCACGCT GGATGCAGGC 
TCCAGCCCCT TCCTTCCTGT GAGCCCAGAG GTCATGAAGC GGAGGCGAGG GGGGCTCATC 
GAGCAGCGGG ACATCATCAA GGCCCACGAG GCACACAAGA TGCAGAGCAC CCCGCAGGCC 
CGGCGCAAGG AGTGGGAGAT GGCTCGCTTC GGGGAGGCGG TGGTCGTTGG CAGGCCAGGC 
TCAGGCGATG GAGACTCGGA CCAGAGCAGG AACCGACAAG GAACCCCCGT CCCGGCCTCG 
GGGCCGGCGG CCGCCTACAA GCAGTCAAAA GCGCAGAGGG CGCGGACTAT GGCTTTGTAC 
AACCCCATAC CAGTCCGGCA GAACTGTTTC ACGGTCAACA GATCCCTGTT CATCTTCGGA 
GAAGATAACA TTGTCAGGAA ATACGCCAAG AAGCTCATCG ATTGGCCGCC ATTTGAGTAC 
ATGATCCTGG CCACCATCAT TGCCAACTGC ATCGTCTTGG CCCTGGAGCA GCATCTTCCT 
GAGGATGACA AGACCCCAAT GTCTCGAAGA CTGGAGAAGA CAGAACCATA TTTCATTGGG 
ATCTTCTGCT TTGAAGCTGG GATCAAAATT GTGGCTCTAG GGTTCATCTT CCATAAGGGT 
TCATACCTCC GTAATGGCTG GAATGTCATG GACTTCATCG TGGTCCTTAG TGGCATCCTG 
GCCACTGCGG GAACCCACTT CAACACCCAC GTGGACCTGC GGACCCTCCG GGCTGTGCGT 
GTCCTGAGGC CTTTAAAGCT GGTATCAGGA ATACCTAGCC TGCAGATCGT GCTGAAGTCT 
ATCATGAAGG CCATGGTGCC TCTTCTGCAG ATTGGCCTTC TGCTCTTCTT TGCCATCCTC 
ATGTTTGCTA TCATTGGCTT GGAGTTCTAC AGTGGCAAGT TGCATCGAGC ATGCTTCATG 
AACAATTCAG GTATTCTAGA GGGGTTTGAT CCTCCTCACC CGTGTGGTGT GCAAGGCTGC 
CCGGCTGGTT ATGAATGTAA GGACTGGATC GGCCCCAATG ACGGGATCAC CCAGTTTGAC 
AACATCCTTT TTGCCGTGCT GACTGTCTTC CAATGCATCA CTATGGAAGG GTGGACCACT 
GTGCTGTACA ATACCAATGA TGCCTTAGGA GCCACCTGGA ACTGGCTGTA CTTCATCCCT 
CTCATCATCA TCGGATCCTT CTTTGTTCTC AACCTCGTCC TGGGAGTGCT TTCCGGGGAA 
TTTGCCAAAG AAAGAGAGAG AGTGGAGAAC CGAAGAGCGT TCATGAAGCT CCGGCGCCAG 
CAACAGATTG AGCGAGAATT GAACGGCTAC CGTGCCTGGA TAGACAAAGC AGAGGAAGTC 
ATGCTTGCTG AAGAAAATAA AAACTCGGGA ACATCAGCCT TGGAAGTGCT TCGAAGGGCA 
ACCATCAAAA GGAGCCGGAC GGAGGCCATG ACCCGAGACT CCAGCGATGA GCACTGCGTT 
GATATCTCTT CAGTGGGCAC ACCTCTTGCC AGAGCCAGTA TCAAGAGCAC AAAAGTAGAT 
GGGGCCTCCT ATTTCCGGCA CAAGGAACGG CTTCTGCGCA TCTCTATCCG CCACATGGTC 
AAGTCCCAAG TCTTTTACTG GATCGTCCTG AGTGTTGTGG CTCTTAATAC TGCCTGTGTG 
GCCATTGTTC ATCACAACCA GCCACAGTGG CTCACTCACC TCCTCTACTA TGCAGAGTTT 
TTGTTTCTGG GACTCTTCCT GTTGGAGATG TCCCTGAAGA TGTATGGCAT GGGGCCACGC 
CTCTATTTTC ACTCATCCTT CAACTGCTTT GATTTTGGGG TCACAGTGGG CAGCATCTTT 
GAGGTTGTCT GGGCAATCTT CAGACCTGGC ACATCTTTTG GAATCAGTGT CTTACGAGCT 
CTCCGGCTCC TAAGGATATT TAAAATAACC AAGTATTGGG CATCCCTCCG GAATCTGGTT 
GTCTCCTTGA TGAGCTCCAT GAAGTCTATC ATCAGCTTAC TCTTCCTTCT CTTCCTCTTC 
ATCGTTGTCT TTGCTCTCCT GGGAATGCAG CTCTTTGGAG GCAGGTTTAA CTTTAATGAT 
GGGACTCCTT CAGCTAATTT TGACACCTTC CCTGCAGCTA TCATGACAGT GTTCCAGATC 
CTGACAGGGG AAGACTGGAA TGAGGTGATG TACAATGGCA TTCGCTCCCA GGGTGGGGTC 
AGCTCAGGCA TGTGGTCTGC CATCTACTTC ATTGTGCTCA CCTTGTTTGG AAACTACACG 
CTGCTAAATG TATTCTTGGC CATCGCTGTG GACAATCTGG CCAATGCCCA GGAACTGACC 
AAGGATGAGC AGGAGGAAGA AGAAGCCTTC AACCAGAAAC ATGCACTGCA GAAGGCCAAG 
GAGGTCAGCC CGATGTCTGC TCCCAACATG CCTTCCATTG AAAGAGACAG AAGGAGAAGA 
CACCACATGT CGATGTGGGA GCCACGCAGC AGCCACCTGA GGGAGCGGAG GCGCCGGCAC 
CACATGTCTG TGTGGGAACA ACGCACCAGC CAGCTGAGGA GGCACATGCA AATGTCTAGC 
CAAGAAGCCC TCAACAAAGA GGAGGCCCCG CCAATGAACC CCCTCAATCC ACTCAACCCA 
CTGAGTCCTC TCAACCCCCT CAATGCTCAC CCCAGCCTTT ATCGGAGGCC CAGGCCCATT 
GAGGGCCTAG CCCTAGGTCT GGGTCTTGAA AAGTGTGAAG AAGAACGCAT CAGTCGTGGG 
GGCTCCCTCA AGGGGGACAT AGGGGGCCTG ACTAGCGCCC TGGACAACCA GAGGAGCCCC 
TTGTCCTTGG GCAAACGGGA GCCACCGTGG CTGCCTAGAT CCTGTCATGG AAACTGTGAC 
CCAATCCAGC AGGAGGCTGG GGGAGGAGAG ACTGTGGTGA CTTTTGAGGA CCGGGCCAGG 
CACAGGCAGA GTCAGAGGCG AAGCCGGCAT CGCCGAGTCA GGACTGAGGG CAAGGACTCT 
GCCTCTGCAT CCCGAAGCAG GTCTGCCAGC CAGGAGCGGA GTCTGGATGA AGGGGTGTCC 
GTTGAGGGGG AGAAGGAACA TGAGCCTCAC AGCAGCCACA GGAGCAAAGA GCCGACCATC 
CACGAAGAGG AGAGAACCCA GGACTTAAGG AGGACCAACA GTCTGATGGT CCCCAGGGGC 
TCTGGACTGG TAGGGGCTCT TGATGAAGCA GAAACACCTC TAGTCCAGCC CCAACCTGAG 
CTGGAAGTGG GGAAGGATGC AGCTCTGACA GAGCAGGAGG CTGAAGGCAG CAGTGAACAA 
GCCCTTCTTG GGGACGTACA GCTGGATGTG GGCCGGGGCA TTAGCCAGAG TGAACCGGAT 
CTCTCCTGCA TGACAGCCAA CATGGACAAG GCTACCACGG AGAGCACCAG TGTCACGGTC 
GCCATCCCTG ATGTGGACCC CTTGGTGGAC TCGACAGTGG TGAACATTAG CAACAAGACG 
GATGGAGAAG CCAGTCCCTT GAAGGAGGCA GAGACTAAAG AGGAAGAAGA AGAGGTAGAG 
AAGAAGAAGA AGCAGAAGAA GGAGAAGCGA GAGACTGGAA AAGCCATGGT GCCCCATAGC 
TCTATGTTCA TCTTCAGCAC CACTAACCCG ATCCGCAGGG CCTGCCACTA CATTGTGAAC 
CTACGCTACT TTGAAATGTG CATTCTCTTG GTGATTGCGG CCAGCAGCAT TGCCCTGGCA 
GCCGAGGACC CTGTACTAAC TAACTCGGAG CGTAACAAAG TCCTGAGATA TTTCGACTAT 
GTTTTCACAG GAGTGTTTAC CTTTGAGATG GTTATAAAGA TGATAGACCA AGGCTTGATC 
CTGCAGGATG GATCATACTT CAGAGACCTC TGGAACATCC TGGACTTTGT AGTAGTGGTT 
GGTGCATTGG TAGCATTTGC TCTGGCGAAC GCTTTGGGAA CCAACAAGGG GAGGGATATC 
AAGACTATCA AGTCTCTGAG GGTACTTCGA GTTCTGCGGC CACTGAAAAC CATCAAGCGC 
TTGCCCAAGC TGAAGGCTGT GTTTGACTGC GTGGTGACCT CCTTGAAGAA TGTCTTCAAC 
ATACTCATTG TCTACAAGCT CTTCATGTTC ATCTTTGCCG TCATCGCTGT TCAGCTATTC 
AAAGGAAAGT TCTTTTATTG CACTGACAGT TCCAAGGACA CAGAGAAGGA GTGCATAGGC 
AACTATGTAG ACCACGAGAA AAACAAGATG GAGGTGAAGG GCCGGGAATG GAAGCGTCAT 
GAATTCCACT ATGACAACAT CATCTGGGCC CTGCTTACCC TCTTCACAGT CTCTACAGGG 
GAAGGATGGC CTCAGGTTCT GCAGCACTCT GTAGATGTGA CAGAGGAGGA CAGAGGCCCA 
AGTCGCAGCA ACCGCATGGA GATGTCCATC TTTTATGTGG TCTACTTTGT GGTCTTCCCT 
TTCTTCTTTG TCAATATCTT CGTGGCTCTC ATTATCATCA CCTTCCAGGA GCAAGGGGAC 
AAGATGATGG AGGAGTGTAG CCTGGAGAAG AATGAGCGGG CATGCATCGA CTTCGCCATT 
AGTGCCAAAC CTCTCACCCG CTACATGCCA CAGAACAGGC ACACCTTCCA GTACCGTGTG 
TGGCACTTTG TAGTCTCCCC ATCCTTTGAG TATACCATCA TGGCCATGAT TGCCTTGAAC 
ACTGTCGTGC TGATGATGAA GTACTACTCT GCTCCTTGCA CCTATGAGCT CGCCTTAAAG 
TACCTGAACA TCGCCTTCAC TATGGTGTTT TCCCTGGAGT GTGTCCTAAA GGTCATTGCT 
TTCGGCTTCC TGAACTATTT CCGAGATACC TGGAACATCT TTGACTTCAT CACGGTGATC 
GGCAGTATCA CAGAAATTAT CCTGACAGAT AGCAAGCTGG TGAACACCAG TGGCTTCAAT 
ATGAGCTTTC TGAAGCTCTT CCGAGCTGCA CGGCTCATAA AGCTTCTGAG ACAGGGCTAC 
ACCATCCGCA TCTTACTGTG GACCTTCGTG CAGTCCTTTA AGGCTCTTCC CTACGTCTGC 
CTTCTCATTG CCATGCTTTT CTTCATCTAC GCCATCATTG GGATGCAGGT GTTTGGAAAC 
ATAAAGTTAG ATGAGGAGAG TCACATCAAC CGGCACAACA ACTTCCGGAG TTTCTTTGGA 
TCCCTCATGC TGCTTTTCAG GAGTGCCACG GGGGAGGCTT GGCAGGAGAT TATGCTGTCC 
TGTCTCGGTG AGAAGGGTTG TGAGCCTGAC ACCACTGCAC CCTCAGGGCA GAATGAGAGT 
GAGCGCTGTG GCACTGACCT GGCCTACGTT TATTTTGTCT CCTTCATCTT CTTCTGCTCC 
TTCCTGATGC TCAACCTGTT CGTGGCTGTC ATCATGGACA ACTTTGAGTA CCTGACTCGA 
GATTCCTCCA TCCTGGGGCC TCACCACTTA GATGAGTTTG TCCGTGTCTG GGCAGAATAT 
GACAGAGCAG CATGCGGCAG GATCCCGTAT AAGGATATGT ACAAATTAGT GCGGGTGATC 
TCGCCTCCTC TGGGCCTGGG AGAGAACTGC CCCTACAGGG TGGCTTGCAA GGTTTGCCCC 
CCCAGTCCCC AGCCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017168356.1
CDS	697..6534
Translation	MVPEMRGLSPGRRLPEPTLCPATGGSPEPGAAGRRRHVLALPDEEAWRPQQCALLEANASDEVGMLPASPRSAVAGFDNFFPVQEGEGPGWEGSTTLDAGSSPFLPVSPEVMKRRRGGLIEQRDIIKAHEAHKMQSTPQARRKEWEMARFGEAVVVGRPGSGDGDSDQSRNRQGTPVPASGPAAAYKQSKAQRARTMALYNPIPVRQNCFTVNRSLFIFGEDNIVRKYAKKLIDWPPFEYMILATIIANCIVLALEQHLPEDDKTPMSRRLEKTEPYFIGIFCFEAGIKIVALGFIFHKGSYLRNGWNVMDFIVVLSGILATAGTHFNTHVDLRTLRAVRVLRPLKLVSGIPSLQIVLKSIMKAMVPLLQIGLLLFFAILMFAIIGLEFYSGKLHRACFMNNSGILEGFDPPHPCGVQGCPAGYECKDWIGPNDGITQFDNILFAVLTVFQCITMEGWTTVLYNTNDALGATWNWLYFIPLIIIGSFFVLNLVLGVLSGEFAKERERVENRRAFMKLRRQQQIERELNGYRAWIDKAEEVMLAEENKNSGTSALEVLRRATIKRSRTEAMTRDSSDEHCVDISSVGTPLARASIKSTKVDGASYFRHKERLLRISIRHMVKSQVFYWIVLSVVALNTACVAIVHHNQPQWLTHLLYYAEFLFLGLFLLEMSLKMYGMGPRLYFHSSFNCFDFGVTVGSIFEVVWAIFRPGTSFGISVLRALRLLRIFKITKYWASLRNLVVSLMSSMKSIISLLFLLFLFIVVFALLGMQLFGGRFNFNDGTPSANFDTFPAAIMTVFQILTGEDWNEVMYNGIRSQGGVSSGMWSAIYFIVLTLFGNYTLLNVFLAIAVDNLANAQELTKDEQEEEEAFNQKHALQKAKEVSPMSAPNMPSIERDRRRRHHMSMWEPRSSHLRERRRRHHMSVWEQRTSQLRRHMQMSSQEALNKEEAPPMNPLNPLNPLSPLNPLNAHPSLYRRPRPIEGLALGLGLEKCEEERISRGGSLKGDIGGLTSALDNQRSPLSLGKREPPWLPRSCHGNCDPIQQEAGGGETVVTFEDRARHRQSQRRSRHRRVRTEGKDSASASRSRSASQERSLDEGVSVEGEKEHEPHSSHRSKEPTIHEEERTQDLRRTNSLMVPRGSGLVGALDEAETPLVQPQPELEVGKDAALTEQEAEGSSEQALLGDVQLDVGRGISQSEPDLSCMTANMDKATTESTSVTVAIPDVDPLVDSTVVNISNKTDGEASPLKEAETKEEEEEVEKKKKQKKEKRETGKAMVPHSSMFIFSTTNPIRRACHYIVNLRYFEMCILLVIAASSIALAAEDPVLTNSERNKVLRYFDYVFTGVFTFEMVIKMIDQGLILQDGSYFRDLWNILDFVVVVGALVAFALANALGTNKGRDIKTIKSLRVLRVLRPLKTIKRLPKLKAVFDCVVTSLKNVFNILIVYKLFMFIFAVIAVQLFKGKFFYCTDSSKDTEKECIGNYVDHEKNKMEVKGREWKRHEFHYDNIIWALLTLFTVSTGEGWPQVLQHSVDVTEEDRGPSRSNRMEMSIFYVVYFVVFPFFFVNIFVALIIITFQEQGDKMMEECSLEKNERACIDFAISAKPLTRYMPQNRHTFQYRVWHFVVSPSFEYTIMAMIALNTVVLMMKYYSAPCTYELALKYLNIAFTMVFSLECVLKVIAFGFLNYFRDTWNIFDFITVIGSITEIILTDSKLVNTSGFNMSFLKLFRAARLIKLLRQGYTIRILLWTFVQSFKALPYVCLLIAMLFFIYAIIGMQVFGNIKLDEESHINRHNNFRSFFGSLMLLFRSATGEAWQEIMLSCLGEKGCEPDTTAPSGQNESERCGTDLAYVYFVSFIFFCSFLMLNLFVAVIMDNFEYLTRDSSILGPHHLDEFVRVWAEYDRAACGRIPYKDMYKLVRVISPPLGLGENCPYRVACKVCPPSPQP

Target ORF information:

RefSeq Version	XM_017312867.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus calcium channel, voltage-dependent, R type, alpha 1E subunit (Cacna1e), transcript variant X21, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017312867.2

ORF Insert Sequence:

ATGGTGCCCG AGATGCGTGG ACTGAGCCCA GGCCGAAGGC TGCCCGAGCC CACCCTGTGC 
CCGGCTACAG GAGGGAGCCC TGAGCCCGGG GCTGCTGGCA GGCGCCGCCA CGTCCTGGCT 
CTGCCTGATG AGGAGGCATG GCGGCCGCAG CAGTGCGCCC TGCTCGAGGC CAATGCCTCC 
GACGAGGTGG GCATGTTGCC TGCTTCCCCG CGCTCCGCGG TGGCCGGCTT CGACAACTTC 
TTCCCGGTGC AGGAAGGCGA GGGCCCGGGC TGGGAGGGCA GCACCACGCT GGATGCAGGC 
TCCAGCCCCT TCCTTCCTGT GAGCCCAGAG GTCATGAAGC GGAGGCGAGG GGGGCTCATC 
GAGCAGCGGG ACATCATCAA GGCCCACGAG GCACACAAGA TGCAGAGCAC CCCGCAGGCC 
CGGCGCAAGG AGTGGGAGAT GGCTCGCTTC GGGGAGGCGG TGGTCGTTGG CAGGCCAGGC 
TCAGGCGATG GAGACTCGGA CCAGAGCAGG AACCGACAAG GAACCCCCGT CCCGGCCTCG 
GGGCCGGCGG CCGCCTACAA GCAGTCAAAA GCGCAGAGGG CGCGGACTAT GGCTTTGTAC 
AACCCCATAC CAGTCCGGCA GAACTGTTTC ACGGTCAACA GATCCCTGTT CATCTTCGGA 
GAAGATAACA TTGTCAGGAA ATACGCCAAG AAGCTCATCG ATTGGCCGCC ATTTGAGTAC 
ATGATCCTGG CCACCATCAT TGCCAACTGC ATCGTCTTGG CCCTGGAGCA GCATCTTCCT 
GAGGATGACA AGACCCCAAT GTCTCGAAGA CTGGAGAAGA CAGAACCATA TTTCATTGGG 
ATCTTCTGCT TTGAAGCTGG GATCAAAATT GTGGCTCTAG GGTTCATCTT CCATAAGGGT 
TCATACCTCC GTAATGGCTG GAATGTCATG GACTTCATCG TGGTCCTTAG TGGCATCCTG 
GCCACTGCGG GAACCCACTT CAACACCCAC GTGGACCTGC GGACCCTCCG GGCTGTGCGT 
GTCCTGAGGC CTTTAAAGCT GGTATCAGGA ATACCTAGCC TGCAGATCGT GCTGAAGTCT 
ATCATGAAGG CCATGGTGCC TCTTCTGCAG ATTGGCCTTC TGCTCTTCTT TGCCATCCTC 
ATGTTTGCTA TCATTGGCTT GGAGTTCTAC AGTGGCAAGT TGCATCGAGC ATGCTTCATG 
AACAATTCAG GTATTCTAGA GGGGTTTGAT CCTCCTCACC CGTGTGGTGT GCAAGGCTGC 
CCGGCTGGTT ATGAATGTAA GGACTGGATC GGCCCCAATG ACGGGATCAC CCAGTTTGAC 
AACATCCTTT TTGCCGTGCT GACTGTCTTC CAATGCATCA CTATGGAAGG GTGGACCACT 
GTGCTGTACA ATACCAATGA TGCCTTAGGA GCCACCTGGA ACTGGCTGTA CTTCATCCCT 
CTCATCATCA TCGGATCCTT CTTTGTTCTC AACCTCGTCC TGGGAGTGCT TTCCGGGGAA 
TTTGCCAAAG AAAGAGAGAG AGTGGAGAAC CGAAGAGCGT TCATGAAGCT CCGGCGCCAG 
CAACAGATTG AGCGAGAATT GAACGGCTAC CGTGCCTGGA TAGACAAAGC AGAGGAAGTC 
ATGCTTGCTG AAGAAAATAA AAACTCGGGA ACATCAGCCT TGGAAGTGCT TCGAAGGGCA 
ACCATCAAAA GGAGCCGGAC GGAGGCCATG ACCCGAGACT CCAGCGATGA GCACTGCGTT 
GATATCTCTT CAGTGGGCAC ACCTCTTGCC AGAGCCAGTA TCAAGAGCAC AAAAGTAGAT 
GGGGCCTCCT ATTTCCGGCA CAAGGAACGG CTTCTGCGCA TCTCTATCCG CCACATGGTC 
AAGTCCCAAG TCTTTTACTG GATCGTCCTG AGTGTTGTGG CTCTTAATAC TGCCTGTGTG 
GCCATTGTTC ATCACAACCA GCCACAGTGG CTCACTCACC TCCTCTACTA TGCAGAGTTT 
TTGTTTCTGG GACTCTTCCT GTTGGAGATG TCCCTGAAGA TGTATGGCAT GGGGCCACGC 
CTCTATTTTC ACTCATCCTT CAACTGCTTT GATTTTGGGG TCACAGTGGG CAGCATCTTT 
GAGGTTGTCT GGGCAATCTT CAGACCTGGC ACATCTTTTG GAATCAGTGT CTTACGAGCT 
CTCCGGCTCC TAAGGATATT TAAAATAACC AAGTATTGGG CATCCCTCCG GAATCTGGTT 
GTCTCCTTGA TGAGCTCCAT GAAGTCTATC ATCAGCTTAC TCTTCCTTCT CTTCCTCTTC 
ATCGTTGTCT TTGCTCTCCT GGGAATGCAG CTCTTTGGAG GCAGGTTTAA CTTTAATGAT 
GGGACTCCTT CAGCTAATTT TGACACCTTC CCTGCAGCTA TCATGACAGT GTTCCAGATC 
CTGACAGGGG AAGACTGGAA TGAGGTGATG TACAATGGCA TTCGCTCCCA GGGTGGGGTC 
AGCTCAGGCA TGTGGTCTGC CATCTACTTC ATTGTGCTCA CCTTGTTTGG AAACTACACG 
CTGCTAAATG TATTCTTGGC CATCGCTGTG GACAATCTGG CCAATGCCCA GGAACTGACC 
AAGGATGAGC AGGAGGAAGA AGAAGCCTTC AACCAGAAAC ATGCACTGCA GAAGGCCAAG 
GAGGTCAGCC CGATGTCTGC TCCCAACATG CCTTCCATTG AAAGAGACAG AAGGAGAAGA 
CACCACATGT CGATGTGGGA GCCACGCAGC AGCCACCTGA GGGAGCGGAG GCGCCGGCAC 
CACATGTCTG TGTGGGAACA ACGCACCAGC CAGCTGAGGA GGCACATGCA AATGTCTAGC 
CAAGAAGCCC TCAACAAAGA GGAGGCCCCG CCAATGAACC CCCTCAATCC ACTCAACCCA 
CTGAGTCCTC TCAACCCCCT CAATGCTCAC CCCAGCCTTT ATCGGAGGCC CAGGCCCATT 
GAGGGCCTAG CCCTAGGTCT GGGTCTTGAA AAGTGTGAAG AAGAACGCAT CAGTCGTGGG 
GGCTCCCTCA AGGGGGACAT AGGGGGCCTG ACTAGCGCCC TGGACAACCA GAGGAGCCCC 
TTGTCCTTGG GCAAACGGGA GCCACCGTGG CTGCCTAGAT CCTGTCATGG AAACTGTGAC 
CCAATCCAGC AGGAGGCTGG GGGAGGAGAG ACTGTGGTGA CTTTTGAGGA CCGGGCCAGG 
CACAGGCAGA GTCAGAGGCG AAGCCGGCAT CGCCGAGTCA GGACTGAGGG CAAGGACTCT 
GCCTCTGCAT CCCGAAGCAG GTCTGCCAGC CAGGAGCGGA GTCTGGATGA AGGGGTGTCC 
GTTGAGGGGG AGAAGGAACA TGAGCCTCAC AGCAGCCACA GGAGCAAAGA GCCGACCATC 
CACGAAGAGG AGAGAACCCA GGACTTAAGG AGGACCAACA GTCTGATGGT CCCCAGGGGC 
TCTGGACTGG TAGGGGCTCT TGATGAAGCA GAAACACCTC TAGTCCAGCC CCAACCTGAG 
CTGGAAGTGG GGAAGGATGC AGCTCTGACA GAGCAGGAGG CTGAAGGCAG CAGTGAACAA 
GCCCTTCTTG GGGACGTACA GCTGGATGTG GGCCGGGGCA TTAGCCAGAG TGAACCGGAT 
CTCTCCTGCA TGACAGCCAA CATGGACAAG GCTACCACGG AGAGCACCAG TGTCACGGTC 
GCCATCCCTG ATGTGGACCC CTTGGTGGAC TCGACAGTGG TGAACATTAG CAACAAGACG 
GATGGAGAAG CCAGTCCCTT GAAGGAGGCA GAGACTAAAG AGGAAGAAGA AGAGGTAGAG 
AAGAAGAAGA AGCAGAAGAA GGAGAAGCGA GAGACTGGAA AAGCCATGGT GCCCCATAGC 
TCTATGTTCA TCTTCAGCAC CACTAACCCG ATCCGCAGGG CCTGCCACTA CATTGTGAAC 
CTACGCTACT TTGAAATGTG CATTCTCTTG GTGATTGCGG CCAGCAGCAT TGCCCTGGCA 
GCCGAGGACC CTGTACTAAC TAACTCGGAG CGTAACAAAG TCCTGAGATA TTTCGACTAT 
GTTTTCACAG GAGTGTTTAC CTTTGAGATG GTTATAAAGA TGATAGACCA AGGCTTGATC 
CTGCAGGATG GATCATACTT CAGAGACCTC TGGAACATCC TGGACTTTGT AGTAGTGGTT 
GGTGCATTGG TAGCATTTGC TCTGGCGAAC GCTTTGGGAA CCAACAAGGG GAGGGATATC 
AAGACTATCA AGTCTCTGAG GGTACTTCGA GTTCTGCGGC CACTGAAAAC CATCAAGCGC 
TTGCCCAAGC TGAAGGCTGT GTTTGACTGC GTGGTGACCT CCTTGAAGAA TGTCTTCAAC 
ATACTCATTG TCTACAAGCT CTTCATGTTC ATCTTTGCCG TCATCGCTGT TCAGCTATTC 
AAAGGAAAGT TCTTTTATTG CACTGACAGT TCCAAGGACA CAGAGAAGGA GTGCATAGGC 
AACTATGTAG ACCACGAGAA AAACAAGATG GAGGTGAAGG GCCGGGAATG GAAGCGTCAT 
GAATTCCACT ATGACAACAT CATCTGGGCC CTGCTTACCC TCTTCACAGT CTCTACAGGG 
GAAGGATGGC CTCAGGTTCT GCAGCACTCT GTAGATGTGA CAGAGGAGGA CAGAGGCCCA 
AGTCGCAGCA ACCGCATGGA GATGTCCATC TTTTATGTGG TCTACTTTGT GGTCTTCCCT 
TTCTTCTTTG TCAATATCTT CGTGGCTCTC ATTATCATCA CCTTCCAGGA GCAAGGGGAC 
AAGATGATGG AGGAGTGTAG CCTGGAGAAG AATGAGCGGG CATGCATCGA CTTCGCCATT 
AGTGCCAAAC CTCTCACCCG CTACATGCCA CAGAACAGGC ACACCTTCCA GTACCGTGTG 
TGGCACTTTG TAGTCTCCCC ATCCTTTGAG TATACCATCA TGGCCATGAT TGCCTTGAAC 
ACTGTCGTGC TGATGATGAA GTACTACTCT GCTCCTTGCA CCTATGAGCT CGCCTTAAAG 
TACCTGAACA TCGCCTTCAC TATGGTGTTT TCCCTGGAGT GTGTCCTAAA GGTCATTGCT 
TTCGGCTTCC TGAACTATTT CCGAGATACC TGGAACATCT TTGACTTCAT CACGGTGATC 
GGCAGTATCA CAGAAATTAT CCTGACAGAT AGCAAGCTGG TGAACACCAG TGGCTTCAAT 
ATGAGCTTTC TGAAGCTCTT CCGAGCTGCA CGGCTCATAA AGCTTCTGAG ACAGGGCTAC 
ACCATCCGCA TCTTACTGTG GACCTTCGTG CAGTCCTTTA AGGCTCTTCC CTACGTCTGC 
CTTCTCATTG CCATGCTTTT CTTCATCTAC GCCATCATTG GGATGCAGGT GTTTGGAAAC 
ATAAAGTTAG ATGAGGAGAG TCACATCAAC CGGCACAACA ACTTCCGGAG TTTCTTTGGA 
TCCCTCATGC TGCTTTTCAG GAGTGCCACG GGGGAGGCTT GGCAGGAGAT TATGCTGTCC 
TGTCTCGGTG AGAAGGGTTG TGAGCCTGAC ACCACTGCAC CCTCAGGGCA GAATGAGAGT 
GAGCGCTGTG GCACTGACCT GGCCTACGTT TATTTTGTCT CCTTCATCTT CTTCTGCTCC 
TTCCTGATGC TCAACCTGTT CGTGGCTGTC ATCATGGACA ACTTTGAGTA CCTGACTCGA 
GATTCCTCCA TCCTGGGGCC TCACCACTTA GATGAGTTTG TCCGTGTCTG GGCAGAATAT 
GACAGAGCAG CATGCGGCAG GATCCCGTAT AAGGATATGT ACAAATTAGT GCGGGTGATC 
TCGCCTCCTC TGGGCCTGGG AGAGAACTGC CCCTACAGGG TGGCTTGCAA GGTTTGCCCC 
CCCAGTCCCC AGCCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cacna1e ( XM_006529105.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006529168.1 Mus musculus calcium channel, voltage-dependent, R type, alpha 1E subunit (Cacna1e), transcript variant X20, mRNA.

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CloneID	OMu27881
Clone ID Related Accession (Same CDS sequence)	XM_006529105.4 , XM_006529105.3
Accession Version	XM_006529105.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5715bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	voltage-dependent R-type calcium channel subunit alpha-1E isoform X17
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006529105.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGCCCG AGATGCGTGG ACTGAGCCCA GGCCGAAGGC TGCCCGAGCC CACCCTGTGC 
CCGGCTACAG GAGGGAGCCC TGAGCCCGGG GCTGCTGGCA GGCGCCGCCA CGTCCTGGCT 
CTGCCTGATG AGGAGGCATG GCGGCCGCAG CAGTGCGCCC TGCTCGAGGC CAATGCCTCC 
GACGAGGTGG GCATGTTGCC TGCTTCCCCG CGCTCCGCGG TGGCCGGCTT CGACAACTTC 
TTCCCGGTGC AGGAAGGCGA GGGCCCGGGC TGGGAGGGCA GCACCACGCT GGATGCAGGC 
TCCAGCCCCT TCCTTCCTGT GAGCCCAGAG GTCATGAAGC GGAGGCGAGG GGGGCTCATC 
GAGCAGCGGG ACATCATCAA GGCCCACGAG GCACACAAGA TGCAGAGCAC CCCGCAGGCC 
CGGCGCAAGG AGTGGGAGAT GGCTCGCTTC GGGGAGGCGG TGGTCGTTGG CAGGCCAGGC 
TCAGGCGATG GAGACTCGGA CCAGAGCAGG AACCGACAAG GAACCCCCGT CCCGGCCTCG 
GGGCCGGCGG CCGCCTACAA GCAGTCAAAA GCGCAGAGGG CGCGGACTAT GGCTTTGTAC 
AACCCCATAC CAGTCCGGCA GAACTGTTTC ACGGTCAACA GATCCCTGTT CATCTTCGGA 
GAAGATAACA TTGTCAGGAA ATACGCCAAG AAGCTCATCG ATTGGCCGCC ATTTGAGTAC 
ATGATCCTGG CCACCATCAT TGCCAACTGC ATCGTCTTGG CCCTGGAGCA GCATCTTCCT 
GAGGATGACA AGACCCCAAT GTCTCGAAGA CTGGAGAAGA CAGAACCATA TTTCATTGGG 
ATCTTCTGCT TTGAAGCTGG GATCAAAATT GTGGCTCTAG GGTTCATCTT CCATAAGGGT 
TCATACCTCC GTAATGGCTG GAATGTCATG GACTTCATCG TGGTCCTTAG TGGCATCCTG 
GCCACTGCGG GAACCCACTT CAACACCCAC GTGGACCTGC GGACCCTCCG GGCTGTGCGT 
GTCCTGAGGC CTTTAAAGCT GGTATCAGGA ATACCTAGCC TGCAGATCGT GCTGAAGTCT 
ATCATGAAGG CCATGGTGCC TCTTCTGCAG ATTGGCCTTC TGCTCTTCTT TGCCATCCTC 
ATGTTTGCTA TCATTGGCTT GGAGTTCTAC AGTGGCAAGT TGCATCGAGC ATGCTTCATG 
AACAATTCAG GTATTCTAGA GGGGTTTGAT CCTCCTCACC CGTGTGGTGT GCAAGGCTGC 
CCGGCTGGTT ATGAATGTAA GGACTGGATC GGCCCCAATG ACGGGATCAC CCAGTTTGAC 
AACATCCTTT TTGCCGTGCT GACTGTCTTC CAATGCATCA CTATGGAAGG GTGGACCACT 
GTGCTGTACA ATACCAATGA TGCCTTAGGA GCCACCTGGA ACTGGCTGTA CTTCATCCCT 
CTCATCATCA TCGGATCCTT CTTTGTTCTC AACCTCGTCC TGGGAGTGCT TTCCGGGGAA 
TTTGCCAAAG AAAGAGAGAG AGTGGAGAAC CGAAGAGCGT TCATGAAGCT CCGGCGCCAG 
CAACAGATTG AGCGAGAATT GAACGGCTAC CGTGCCTGGA TAGACAAAGC AGAGGAAGTC 
ATGCTTGCTG AAGAAAATAA AAACTCGGGA ACATCAGCCT TGGAAGTGCT TCGAAGGGCA 
ACCATCAAAA GGAGCCGGAC GGAGGCCATG ACCCGAGACT CCAGCGATGA GCACTGCGTT 
GATATCTCTT CAGTGGGCAC ACCTCTTGCC AGAGCCAGTA TCAAGAGCAC AAAAGTAGAT 
GGGGCCTCCT ATTTCCGGCA CAAGGAACGG CTTCTGCGCA TCTCTATCCG CCACATGGTC 
AAGTCCCAAG TCTTTTACTG GATCGTCCTG AGTGTTGTGG CTCTTAATAC TGCCTGTGTG 
GCCATTGTTC ATCACAACCA GCCACAGTGG CTCACTCACC TCCTCTACTA TGCAGAGTTT 
TTGTTTCTGG GACTCTTCCT GTTGGAGATG TCCCTGAAGA TGTATGGCAT GGGGCCACGC 
CTCTATTTTC ACTCATCCTT CAACTGCTTT GATTTTGGGG TCACAGTGGG CAGCATCTTT 
GAGGTTGTCT GGGCAATCTT CAGACCTGGC ACATCTTTTG GAATCAGTGT CTTACGAGCT 
CTCCGGCTCC TAAGGATATT TAAAATAACC AAGTATTGGG CATCCCTCCG GAATCTGGTT 
GTCTCCTTGA TGAGCTCCAT GAAGTCTATC ATCAGCTTAC TCTTCCTTCT CTTCCTCTTC 
ATCGTTGTCT TTGCTCTCCT GGGAATGCAG CTCTTTGGAG GCAGGTTTAA CTTTAATGAT 
GGGACTCCTT CAGCTAATTT TGACACCTTC CCTGCAGCTA TCATGACAGT GTTCCAGATC 
CTGACAGGGG AAGACTGGAA TGAGGTGATG TACAATGGCA TTCGCTCCCA GGGTGGGGTC 
AGCTCAGGCA TGTGGTCTGC CATCTACTTC ATTGTGCTCA CCTTGTTTGG AAACTACACG 
CTGCTAAATG TATTCTTGGC CATCGCTGTG GACAATCTGG CCAATGCCCA GGAACTGACC 
AAGGATGAGC AGGAGGAAGA AGAAGCCTTC AACCAGAAAC ATGCACTGCA GAAGGCCAAG 
GAGGTCAGCC CGATGTCTGC TCCCAACATG CCTTCCATTG AAAGAGACAG AAGGAGAAGA 
CACCACATGT CGATGTGGGA GCCACGCAGC AGCCACCTGA GGGAGCGGAG GCGCCGGCAC 
CACATGTCTG TGTGGGAACA ACGCACCAGC CAGCTGAGGA GGCACATGCA AATGTCTAGC 
CAAGAAGCCC TCAACAAAGA GGAGGCCCCG CCAATGAACC CCCTCAATCC ACTCAACCCA 
CTGAGTCCTC TCAACCCCCT CAATGCTCAC CCCAGCCTTT ATCGGAGGCC CAGGCCCATT 
GAGGGCCTAG CCCTAGGTCT GGGTCTTGAA AAGTGTGAAG AAGAACGCAT CAGTCGTGGG 
GGCTCCCTCA AGGGGGACAT AGGGGGCCTG ACTAGCGCCC TGGACAACCA GAGGAGCCCC 
TTGTCCTTGG GCAAACGGGA GCCACCGTGG CTGCCTAGAT CCTGTCATGG AAACTGTGAC 
CCAATCCAGC AGGAGGCTGG GGGAGGAGAG ACTGTGGTGA CTTTTGAGGA CCGGGCCAGG 
CACAGGCAGA GTCAGAGGCG AAGCCGGCAT CGCCGAGTCA GGACTGAGGG CAAGGACTCT 
GCCTCTGCAT CCCGAAGCAG GTCTGCCAGC CAGGAGCGGA GTCTGGATGA AGGGGTGTCC 
GTTGAGGGGG AGAAGGAACA TGAGCCTCAC AGCAGCCACA GGAGCAAAGA GCCGACCATC 
CACGAAGAGG AGAGAACCCA GGACTTAAGG AGGACCAACA GTCTGATGGT CCCCAGGGGC 
TCTGGACTGG TAGGGGCTCT TGATGAAGCA GAAACACCTC TAGTCCAGCC CCAACCTGAG 
CTGGAAGTGG GGAAGGATGC AGCTCTGACA GAGCAGGAGG CTGAAGGCAG CAGTGAACAA 
GCCCTTCTTG GGGACGTACA GCTGGATGTG GGCCGGGGCA TTAGCCAGAG TGAACCGGAT 
CTCTCCTGCA TGACAGCCAA CATGGACAAG GCTACCACGG AGAGCACCAG TGTCACGGTC 
GCCATCCCTG ATGTGGACCC CTTGGTGGAC TCGACAGTGG TGAACATTAG CAACAAGACG 
GATGGAGAAG CCAGTCCCTT GAAGGAGGCA GAGACTAAAG AGGAAGAAGA AGAGGTAGAG 
AAGAAGAAGA AGCAGAAGAA GGAGAAGCGA GAGACTGGAA AAGCCATGGT GCCCCATAGC 
TCTATGTTCA TCTTCAGCAC CACTAACCCG ATCCGCAGGG CCTGCCACTA CATTGTGAAC 
CTACGCTACT TTGAAATGTG CATTCTCTTG GTGATTGCGG CCAGCAGCAT TGCCCTGGCA 
GCCGAGGACC CTGTACTAAC TAACTCGGAG CGTAACAAAG TCCTGAGATA TTTCGACTAT 
GTTTTCACAG GAGTGTTTAC CTTTGAGATG GTTATAAAGA TGATAGACCA AGGCTTGATC 
CTGCAGGATG GATCATACTT CAGAGACCTC TGGAACATCC TGGACTTTGT AGTAGTGGTT 
GGTGCATTGG TAGCATTTGC TCTGGCGAAC GCTTTGGGAA CCAACAAGGG GAGGGATATC 
AAGACTATCA AGTCTCTGAG GGTACTTCGA GTTCTGCGGC CACTGAAAAC CATCAAGCGC 
TTGCCCAAGC TGAAGGCTGT GTTTGACTGC GTGGTGACCT CCTTGAAGAA TGTCTTCAAC 
ATACTCATTG TCTACAAGCT CTTCATGTTC ATCTTTGCCG TCATCGCTGT TCAGCTATTC 
AAAGGAAAGT TCTTTTATTG CACTGACAGT TCCAAGGACA CAGAGAAGGA GTGCATAGGC 
AACTATGTAG ACCACGAGAA AAACAAGATG GAGGTGAAGG GCCGGGAATG GAAGCGTCAT 
GAATTCCACT ATGACAACAT CATCTGGGCC CTGCTTACCC TCTTCACAGT CTCTACAGGG 
GAAGGATGGC CTCAGGTTCT GCAGCACTCT GTAGATGTGA CAGAGGAGGA CAGAGGCCCA 
AGTCGCAGCA ACCGCATGGA GATGTCCATC TTTTATGTGG TCTACTTTGT GGTCTTCCCT 
TTCTTCTTTG TCAATATCTT CGTGGCTCTC ATTATCATCA CCTTCCAGGA GCAAGGGGAC 
AAGATGATGG AGGAGTGTAG CCTGGAGAAG AATGAGCGGG CATGCATCGA CTTCGCCATT 
AGTGCCAAAC CTCTCACCCG CTACATGCCA CAGAACAGGC ACACCTTCCA GTACCGTGTG 
TGGCACTTTG TAGTCTCCCC ATCCTTTGAG TATACCATCA TGGCCATGAT TGCCTTGAAC 
ACTGTCGTGC TGATGATGAA GTACTACTCT GCTCCTTGCA CCTATGAGCT CGCCTTAAAG 
TACCTGAACA TCGCCTTCAC TATGGTGTTT TCCCTGGAGT GTGTCCTAAA GGTCATTGCT 
TTCGGCTTCC TGAACTATTT CCGAGATACC TGGAACATCT TTGACTTCAT CACGGTGATC 
GGCAGTATCA CAGAAATTAT CCTGACAGAT AGCAAGCTGG TGAACACCAG TGGCTTCAAT 
ATGAGCTTTC TGAAGCTCTT CCGAGCTGCA CGGCTCATAA AGCTTCTGAG ACAGGGCTAC 
ACCATCCGCA TCTTACTGTG GACCTTCGTG CAGTCCTTTA AGGCTCTTCC CTACGTCTGC 
CTTCTCATTG CCATGCTTTT CTTCATCTAC GCCATCATTG GGATGCAGGT GTTTGGAAAC 
ATAAAGTTAG ATGAGGAGAG TCACATCAAC CGGCACAACA ACTTCCGGAG TTTCTTTGGA 
TCCCTCATGC TGCTTTTCAG GAGTGCCACG GGGGAGGCTT GGCAGGAGAT TATGCTGTCC 
TGTCTCGGTG AGAAGGGTTG TGAGCCTGAC ACCACTGCAC CCTCAGGGCA GAATGAGAGT 
GAGCGCTGTG GCACTGACCT GGCCTACGTT TATTTTGTCT CCTTCATCTT CTTCTGCTCC 
TTCCTGATGC TCAACCTGTT CGTGGCTGTC ATCATGGACA ACTTTGAGTA CCTGACTCGA 
GATTCCTCCA TCCTGGGGCC TCACCACTTA GATGAGTTTG TCCGTGTCTG GGCAGAATAT 
GACAGAGCAG CATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006529168.1
CDS	697..6411
Translation	MVPEMRGLSPGRRLPEPTLCPATGGSPEPGAAGRRRHVLALPDEEAWRPQQCALLEANASDEVGMLPASPRSAVAGFDNFFPVQEGEGPGWEGSTTLDAGSSPFLPVSPEVMKRRRGGLIEQRDIIKAHEAHKMQSTPQARRKEWEMARFGEAVVVGRPGSGDGDSDQSRNRQGTPVPASGPAAAYKQSKAQRARTMALYNPIPVRQNCFTVNRSLFIFGEDNIVRKYAKKLIDWPPFEYMILATIIANCIVLALEQHLPEDDKTPMSRRLEKTEPYFIGIFCFEAGIKIVALGFIFHKGSYLRNGWNVMDFIVVLSGILATAGTHFNTHVDLRTLRAVRVLRPLKLVSGIPSLQIVLKSIMKAMVPLLQIGLLLFFAILMFAIIGLEFYSGKLHRACFMNNSGILEGFDPPHPCGVQGCPAGYECKDWIGPNDGITQFDNILFAVLTVFQCITMEGWTTVLYNTNDALGATWNWLYFIPLIIIGSFFVLNLVLGVLSGEFAKERERVENRRAFMKLRRQQQIERELNGYRAWIDKAEEVMLAEENKNSGTSALEVLRRATIKRSRTEAMTRDSSDEHCVDISSVGTPLARASIKSTKVDGASYFRHKERLLRISIRHMVKSQVFYWIVLSVVALNTACVAIVHHNQPQWLTHLLYYAEFLFLGLFLLEMSLKMYGMGPRLYFHSSFNCFDFGVTVGSIFEVVWAIFRPGTSFGISVLRALRLLRIFKITKYWASLRNLVVSLMSSMKSIISLLFLLFLFIVVFALLGMQLFGGRFNFNDGTPSANFDTFPAAIMTVFQILTGEDWNEVMYNGIRSQGGVSSGMWSAIYFIVLTLFGNYTLLNVFLAIAVDNLANAQELTKDEQEEEEAFNQKHALQKAKEVSPMSAPNMPSIERDRRRRHHMSMWEPRSSHLRERRRRHHMSVWEQRTSQLRRHMQMSSQEALNKEEAPPMNPLNPLNPLSPLNPLNAHPSLYRRPRPIEGLALGLGLEKCEEERISRGGSLKGDIGGLTSALDNQRSPLSLGKREPPWLPRSCHGNCDPIQQEAGGGETVVTFEDRARHRQSQRRSRHRRVRTEGKDSASASRSRSASQERSLDEGVSVEGEKEHEPHSSHRSKEPTIHEEERTQDLRRTNSLMVPRGSGLVGALDEAETPLVQPQPELEVGKDAALTEQEAEGSSEQALLGDVQLDVGRGISQSEPDLSCMTANMDKATTESTSVTVAIPDVDPLVDSTVVNISNKTDGEASPLKEAETKEEEEEVEKKKKQKKEKRETGKAMVPHSSMFIFSTTNPIRRACHYIVNLRYFEMCILLVIAASSIALAAEDPVLTNSERNKVLRYFDYVFTGVFTFEMVIKMIDQGLILQDGSYFRDLWNILDFVVVVGALVAFALANALGTNKGRDIKTIKSLRVLRVLRPLKTIKRLPKLKAVFDCVVTSLKNVFNILIVYKLFMFIFAVIAVQLFKGKFFYCTDSSKDTEKECIGNYVDHEKNKMEVKGREWKRHEFHYDNIIWALLTLFTVSTGEGWPQVLQHSVDVTEEDRGPSRSNRMEMSIFYVVYFVVFPFFFVNIFVALIIITFQEQGDKMMEECSLEKNERACIDFAISAKPLTRYMPQNRHTFQYRVWHFVVSPSFEYTIMAMIALNTVVLMMKYYSAPCTYELALKYLNIAFTMVFSLECVLKVIAFGFLNYFRDTWNIFDFITVIGSITEIILTDSKLVNTSGFNMSFLKLFRAARLIKLLRQGYTIRILLWTFVQSFKALPYVCLLIAMLFFIYAIIGMQVFGNIKLDEESHINRHNNFRSFFGSLMLLFRSATGEAWQEIMLSCLGEKGCEPDTTAPSGQNESERCGTDLAYVYFVSFIFFCSFLMLNLFVAVIMDNFEYLTRDSSILGPHHLDEFVRVWAEYDRAA

Target ORF information:

RefSeq Version	XM_006529105.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus calcium channel, voltage-dependent, R type, alpha 1E subunit (Cacna1e), transcript variant X20, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006529105.3

ORF Insert Sequence:

ATGGTGCCCG AGATGCGTGG ACTGAGCCCA GGCCGAAGGC TGCCCGAGCC CACCCTGTGC 
CCGGCTACAG GAGGGAGCCC TGAGCCCGGG GCTGCTGGCA GGCGCCGCCA CGTCCTGGCT 
CTGCCTGATG AGGAGGCATG GCGGCCGCAG CAGTGCGCCC TGCTCGAGGC CAATGCCTCC 
GACGAGGTGG GCATGTTGCC TGCTTCCCCG CGCTCCGCGG TGGCCGGCTT CGACAACTTC 
TTCCCGGTGC AGGAAGGCGA GGGCCCGGGC TGGGAGGGCA GCACCACGCT GGATGCAGGC 
TCCAGCCCCT TCCTTCCTGT GAGCCCAGAG GTCATGAAGC GGAGGCGAGG GGGGCTCATC 
GAGCAGCGGG ACATCATCAA GGCCCACGAG GCACACAAGA TGCAGAGCAC CCCGCAGGCC 
CGGCGCAAGG AGTGGGAGAT GGCTCGCTTC GGGGAGGCGG TGGTCGTTGG CAGGCCAGGC 
TCAGGCGATG GAGACTCGGA CCAGAGCAGG AACCGACAAG GAACCCCCGT CCCGGCCTCG 
GGGCCGGCGG CCGCCTACAA GCAGTCAAAA GCGCAGAGGG CGCGGACTAT GGCTTTGTAC 
AACCCCATAC CAGTCCGGCA GAACTGTTTC ACGGTCAACA GATCCCTGTT CATCTTCGGA 
GAAGATAACA TTGTCAGGAA ATACGCCAAG AAGCTCATCG ATTGGCCGCC ATTTGAGTAC 
ATGATCCTGG CCACCATCAT TGCCAACTGC ATCGTCTTGG CCCTGGAGCA GCATCTTCCT 
GAGGATGACA AGACCCCAAT GTCTCGAAGA CTGGAGAAGA CAGAACCATA TTTCATTGGG 
ATCTTCTGCT TTGAAGCTGG GATCAAAATT GTGGCTCTAG GGTTCATCTT CCATAAGGGT 
TCATACCTCC GTAATGGCTG GAATGTCATG GACTTCATCG TGGTCCTTAG TGGCATCCTG 
GCCACTGCGG GAACCCACTT CAACACCCAC GTGGACCTGC GGACCCTCCG GGCTGTGCGT 
GTCCTGAGGC CTTTAAAGCT GGTATCAGGA ATACCTAGCC TGCAGATCGT GCTGAAGTCT 
ATCATGAAGG CCATGGTGCC TCTTCTGCAG ATTGGCCTTC TGCTCTTCTT TGCCATCCTC 
ATGTTTGCTA TCATTGGCTT GGAGTTCTAC AGTGGCAAGT TGCATCGAGC ATGCTTCATG 
AACAATTCAG GTATTCTAGA GGGGTTTGAT CCTCCTCACC CGTGTGGTGT GCAAGGCTGC 
CCGGCTGGTT ATGAATGTAA GGACTGGATC GGCCCCAATG ACGGGATCAC CCAGTTTGAC 
AACATCCTTT TTGCCGTGCT GACTGTCTTC CAATGCATCA CTATGGAAGG GTGGACCACT 
GTGCTGTACA ATACCAATGA TGCCTTAGGA GCCACCTGGA ACTGGCTGTA CTTCATCCCT 
CTCATCATCA TCGGATCCTT CTTTGTTCTC AACCTCGTCC TGGGAGTGCT TTCCGGGGAA 
TTTGCCAAAG AAAGAGAGAG AGTGGAGAAC CGAAGAGCGT TCATGAAGCT CCGGCGCCAG 
CAACAGATTG AGCGAGAATT GAACGGCTAC CGTGCCTGGA TAGACAAAGC AGAGGAAGTC 
ATGCTTGCTG AAGAAAATAA AAACTCGGGA ACATCAGCCT TGGAAGTGCT TCGAAGGGCA 
ACCATCAAAA GGAGCCGGAC GGAGGCCATG ACCCGAGACT CCAGCGATGA GCACTGCGTT 
GATATCTCTT CAGTGGGCAC ACCTCTTGCC AGAGCCAGTA TCAAGAGCAC AAAAGTAGAT 
GGGGCCTCCT ATTTCCGGCA CAAGGAACGG CTTCTGCGCA TCTCTATCCG CCACATGGTC 
AAGTCCCAAG TCTTTTACTG GATCGTCCTG AGTGTTGTGG CTCTTAATAC TGCCTGTGTG 
GCCATTGTTC ATCACAACCA GCCACAGTGG CTCACTCACC TCCTCTACTA TGCAGAGTTT 
TTGTTTCTGG GACTCTTCCT GTTGGAGATG TCCCTGAAGA TGTATGGCAT GGGGCCACGC 
CTCTATTTTC ACTCATCCTT CAACTGCTTT GATTTTGGGG TCACAGTGGG CAGCATCTTT 
GAGGTTGTCT GGGCAATCTT CAGACCTGGC ACATCTTTTG GAATCAGTGT CTTACGAGCT 
CTCCGGCTCC TAAGGATATT TAAAATAACC AAGTATTGGG CATCCCTCCG GAATCTGGTT 
GTCTCCTTGA TGAGCTCCAT GAAGTCTATC ATCAGCTTAC TCTTCCTTCT CTTCCTCTTC 
ATCGTTGTCT TTGCTCTCCT GGGAATGCAG CTCTTTGGAG GCAGGTTTAA CTTTAATGAT 
GGGACTCCTT CAGCTAATTT TGACACCTTC CCTGCAGCTA TCATGACAGT GTTCCAGATC 
CTGACAGGGG AAGACTGGAA TGAGGTGATG TACAATGGCA TTCGCTCCCA GGGTGGGGTC 
AGCTCAGGCA TGTGGTCTGC CATCTACTTC ATTGTGCTCA CCTTGTTTGG AAACTACACG 
CTGCTAAATG TATTCTTGGC CATCGCTGTG GACAATCTGG CCAATGCCCA GGAACTGACC 
AAGGATGAGC AGGAGGAAGA AGAAGCCTTC AACCAGAAAC ATGCACTGCA GAAGGCCAAG 
GAGGTCAGCC CGATGTCTGC TCCCAACATG CCTTCCATTG AAAGAGACAG AAGGAGAAGA 
CACCACATGT CGATGTGGGA GCCACGCAGC AGCCACCTGA GGGAGCGGAG GCGCCGGCAC 
CACATGTCTG TGTGGGAACA ACGCACCAGC CAGCTGAGGA GGCACATGCA AATGTCTAGC 
CAAGAAGCCC TCAACAAAGA GGAGGCCCCG CCAATGAACC CCCTCAATCC ACTCAACCCA 
CTGAGTCCTC TCAACCCCCT CAATGCTCAC CCCAGCCTTT ATCGGAGGCC CAGGCCCATT 
GAGGGCCTAG CCCTAGGTCT GGGTCTTGAA AAGTGTGAAG AAGAACGCAT CAGTCGTGGG 
GGCTCCCTCA AGGGGGACAT AGGGGGCCTG ACTAGCGCCC TGGACAACCA GAGGAGCCCC 
TTGTCCTTGG GCAAACGGGA GCCACCGTGG CTGCCTAGAT CCTGTCATGG AAACTGTGAC 
CCAATCCAGC AGGAGGCTGG GGGAGGAGAG ACTGTGGTGA CTTTTGAGGA CCGGGCCAGG 
CACAGGCAGA GTCAGAGGCG AAGCCGGCAT CGCCGAGTCA GGACTGAGGG CAAGGACTCT 
GCCTCTGCAT CCCGAAGCAG GTCTGCCAGC CAGGAGCGGA GTCTGGATGA AGGGGTGTCC 
GTTGAGGGGG AGAAGGAACA TGAGCCTCAC AGCAGCCACA GGAGCAAAGA GCCGACCATC 
CACGAAGAGG AGAGAACCCA GGACTTAAGG AGGACCAACA GTCTGATGGT CCCCAGGGGC 
TCTGGACTGG TAGGGGCTCT TGATGAAGCA GAAACACCTC TAGTCCAGCC CCAACCTGAG 
CTGGAAGTGG GGAAGGATGC AGCTCTGACA GAGCAGGAGG CTGAAGGCAG CAGTGAACAA 
GCCCTTCTTG GGGACGTACA GCTGGATGTG GGCCGGGGCA TTAGCCAGAG TGAACCGGAT 
CTCTCCTGCA TGACAGCCAA CATGGACAAG GCTACCACGG AGAGCACCAG TGTCACGGTC 
GCCATCCCTG ATGTGGACCC CTTGGTGGAC TCGACAGTGG TGAACATTAG CAACAAGACG 
GATGGAGAAG CCAGTCCCTT GAAGGAGGCA GAGACTAAAG AGGAAGAAGA AGAGGTAGAG 
AAGAAGAAGA AGCAGAAGAA GGAGAAGCGA GAGACTGGAA AAGCCATGGT GCCCCATAGC 
TCTATGTTCA TCTTCAGCAC CACTAACCCG ATCCGCAGGG CCTGCCACTA CATTGTGAAC 
CTACGCTACT TTGAAATGTG CATTCTCTTG GTGATTGCGG CCAGCAGCAT TGCCCTGGCA 
GCCGAGGACC CTGTACTAAC TAACTCGGAG CGTAACAAAG TCCTGAGATA TTTCGACTAT 
GTTTTCACAG GAGTGTTTAC CTTTGAGATG GTTATAAAGA TGATAGACCA AGGCTTGATC 
CTGCAGGATG GATCATACTT CAGAGACCTC TGGAACATCC TGGACTTTGT AGTAGTGGTT 
GGTGCATTGG TAGCATTTGC TCTGGCGAAC GCTTTGGGAA CCAACAAGGG GAGGGATATC 
AAGACTATCA AGTCTCTGAG GGTACTTCGA GTTCTGCGGC CACTGAAAAC CATCAAGCGC 
TTGCCCAAGC TGAAGGCTGT GTTTGACTGC GTGGTGACCT CCTTGAAGAA TGTCTTCAAC 
ATACTCATTG TCTACAAGCT CTTCATGTTC ATCTTTGCCG TCATCGCTGT TCAGCTATTC 
AAAGGAAAGT TCTTTTATTG CACTGACAGT TCCAAGGACA CAGAGAAGGA GTGCATAGGC 
AACTATGTAG ACCACGAGAA AAACAAGATG GAGGTGAAGG GCCGGGAATG GAAGCGTCAT 
GAATTCCACT ATGACAACAT CATCTGGGCC CTGCTTACCC TCTTCACAGT CTCTACAGGG 
GAAGGATGGC CTCAGGTTCT GCAGCACTCT GTAGATGTGA CAGAGGAGGA CAGAGGCCCA 
AGTCGCAGCA ACCGCATGGA GATGTCCATC TTTTATGTGG TCTACTTTGT GGTCTTCCCT 
TTCTTCTTTG TCAATATCTT CGTGGCTCTC ATTATCATCA CCTTCCAGGA GCAAGGGGAC 
AAGATGATGG AGGAGTGTAG CCTGGAGAAG AATGAGCGGG CATGCATCGA CTTCGCCATT 
AGTGCCAAAC CTCTCACCCG CTACATGCCA CAGAACAGGC ACACCTTCCA GTACCGTGTG 
TGGCACTTTG TAGTCTCCCC ATCCTTTGAG TATACCATCA TGGCCATGAT TGCCTTGAAC 
ACTGTCGTGC TGATGATGAA GTACTACTCT GCTCCTTGCA CCTATGAGCT CGCCTTAAAG 
TACCTGAACA TCGCCTTCAC TATGGTGTTT TCCCTGGAGT GTGTCCTAAA GGTCATTGCT 
TTCGGCTTCC TGAACTATTT CCGAGATACC TGGAACATCT TTGACTTCAT CACGGTGATC 
GGCAGTATCA CAGAAATTAT CCTGACAGAT AGCAAGCTGG TGAACACCAG TGGCTTCAAT 
ATGAGCTTTC TGAAGCTCTT CCGAGCTGCA CGGCTCATAA AGCTTCTGAG ACAGGGCTAC 
ACCATCCGCA TCTTACTGTG GACCTTCGTG CAGTCCTTTA AGGCTCTTCC CTACGTCTGC 
CTTCTCATTG CCATGCTTTT CTTCATCTAC GCCATCATTG GGATGCAGGT GTTTGGAAAC 
ATAAAGTTAG ATGAGGAGAG TCACATCAAC CGGCACAACA ACTTCCGGAG TTTCTTTGGA 
TCCCTCATGC TGCTTTTCAG GAGTGCCACG GGGGAGGCTT GGCAGGAGAT TATGCTGTCC 
TGTCTCGGTG AGAAGGGTTG TGAGCCTGAC ACCACTGCAC CCTCAGGGCA GAATGAGAGT 
GAGCGCTGTG GCACTGACCT GGCCTACGTT TATTTTGTCT CCTTCATCTT CTTCTGCTCC 
TTCCTGATGC TCAACCTGTT CGTGGCTGTC ATCATGGACA ACTTTGAGTA CCTGACTCGA 
GATTCCTCCA TCCTGGGGCC TCACCACTTA GATGAGTTTG TCCGTGTCTG GGCAGAATAT 
GACAGAGCAG CATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cacna1e ( XM_030243926.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030099786.1 Mus musculus calcium channel, voltage-dependent, R type, alpha 1E subunit (Cacna1e), transcript variant X3, mRNA.

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CloneID	OMu67260
Clone ID Related Accession (Same CDS sequence)	XM_017312578.1 , XM_030243926.1 , XM_017312594.1 , XM_030243929.1 , XM_011247909.2 , XM_017312578.2 , XM_011247909.3
Accession Version	XM_030243926.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7401bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	voltage-dependent R-type calcium channel subunit alpha-1E isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGCCCG AGATGCGTGG ACTGAGCCCA GGCCGAAGGC TGCCCGAGCC CACCCTGTGC 
CCGGCTACAG GAGGGAGCCC TGAGCCCGGG GCTGCTGGCA GGCGCCGCCA CGTCCTGGCT 
CTGCCTGATG AGGAGGCATG GCGGCCGCAG CAGTGCGCCC TGCTCGAGGC CAATGCCTCC 
GACGAGGTGG GCATGTTGCC TGCTTCCCCG CGCTCCGCGG TGGCCGGCTT CGACAACTTC 
TTCCCGGTGC AGGAAGGCGA GGGCCCGGGC TGGGAGGGCA GCACCACGCT GGATGCAGGC 
TCCAGCCCCT TCCTTCCTGT GAGCCCAGAG GTCATGAAGC GGAGGCGAGG GGGGCTCATC 
GAGCAGCGGG ACATCATCAA GGCCCACGAG GCACACAAGA TGCAGAGCAC CCCGCAGGCC 
CGGCGCAAGG AGTGGGAGAT GGCTCGCTTC GGGGAGGCGG TGGTCGTTGG CAGGCCAGGC 
TCAGGCGATG GAGACTCGGA CCAGAGCAGG AACCGACAAG GAACCCCCGT CCCGGCCTCG 
GGGCCGGCGG CCGCCTACAA GCAGTCAAAA GCGCAGAGGG CGCGGACTAT GGCTTTGTAC 
AACCCCATAC CAGTCCGGCA GAACTGTTTC ACGGTCAACA GATCCCTGTT CATCTTCGGA 
GAAGATAACA TTGTCAGGAA ATACGCCAAG AAGCTCATCG ATTGGCCGCC ATTTGAGTAC 
ATGATCCTGG CCACCATCAT TGCCAACTGC ATCGTCTTGG CCCTGGAGCA GCATCTTCCT 
GAGGATGACA AGACCCCAAT GTCTCGAAGA CTGGAGAAGA CAGAACCATA TTTCATTGGG 
ATCTTCTGCT TTGAAGCTGG GATCAAAATT GTGGCTCTAG GGTTCATCTT CCATAAGGGT 
TCATACCTCC GTAATGGCTG GAATGTCATG GACTTCATCG TGGTCCTTAG TGGCATCCTG 
GCCACTGCGG GAACCCACTT CAACACCCAC GTGGACCTGC GGACCCTCCG GGCTGTGCGT 
GTCCTGAGGC CTTTAAAGCT GGTATCAGGA ATACCTAGCC TGCAGATCGT GCTGAAGTCT 
ATCATGAAGG CCATGGTGCC TCTTCTGCAG ATTGGCCTTC TGCTCTTCTT TGCCATCCTC 
ATGTTTGCTA TCATTGGCTT GGAGTTCTAC AGTGGCAAGT TGCATCGAGC ATGCTTCATG 
AACAATTCAG GTATTCTAGA GGGGTTTGAT CCTCCTCACC CGTGTGGTGT GCAAGGCTGC 
CCGGCTGGTT ATGAATGTAA GGACTGGATC GGCCCCAATG ACGGGATCAC CCAGTTTGAC 
AACATCCTTT TTGCCGTGCT GACTGTCTTC CAATGCATCA CTATGGAAGG GTGGACCACT 
GTGCTGTACA ATACCAATGA TGCCTTAGGA GCCACCTGGA ACTGGCTGTA CTTCATCCCT 
CTCATCATCA TCGGATCCTT CTTTGTTCTC AACCTCGTCC TGGGAGTGCT TTCCGGGGAA 
TTTGCCAAAG AAAGAGAGAG AGTGGAGAAC CGAAGAGCGT TCATGAAGCT CCGGCGCCAG 
CAACAGATTG AGCGAGAATT GAACGGCTAC CGTGCCTGGA TAGACAAAGC AGAGGAAGTC 
ATGCTTGCTG AAGAAAATAA AAACTCGGGA ACATCAGCCT TGGAAGTGCT TCGAAGGGCA 
ACCATCAAAA GGAGCCGGAC GGAGGCCATG ACCCGAGACT CCAGCGATGA GCACTGCGTT 
GATATCTCTT CAGTGGGCAC ACCTCTTGCC AGAGCCAGTA TCAAGAGCAC AAAAGTAGAT 
GGGGCCTCCT ATTTCCGGCA CAAGGAACGG CTTCTGCGCA TCTCTATCCG CCACATGGTC 
AAGTCCCAAG TCTTTTACTG GATCGTCCTG AGTGTTGTGG CTCTTAATAC TGCCTGTGTG 
GCCATTGTTC ATCACAACCA GCCACAGTGG CTCACTCACC TCCTCTACTA TGCAGAGTTT 
TTGTTTCTGG GACTCTTCCT GTTGGAGATG TCCCTGAAGA TGTATGGCAT GGGGCCACGC 
CTCTATTTTC ACTCATCCTT CAACTGCTTT GATTTTGGGG TCACAGTGGG CAGCATCTTT 
GAGGTTGTCT GGGCAATCTT CAGACCTGGC ACATCTTTTG GAATCAGTGT CTTACGAGCT 
CTCCGGCTCC TAAGGATATT TAAAATAACC AAGTATTGGG CATCCCTCCG GAATCTGGTT 
GTCTCCTTGA TGAGCTCCAT GAAGTCTATC ATCAGCTTAC TCTTCCTTCT CTTCCTCTTC 
ATCGTTGTCT TTGCTCTCCT GGGAATGCAG CTCTTTGGAG GCAGGTTTAA CTTTAATGAT 
GGGACTCCTT CAGCTAATTT TGACACCTTC CCTGCAGCTA TCATGACAGT GTTCCAGATC 
CTGACAGGGG AAGACTGGAA TGAGGTGATG TACAATGGCA TTCGCTCCCA GGGTGGGGTC 
AGCTCAGGCA TGTGGTCTGC CATCTACTTC ATTGTGCTCA CCTTGTTTGG AAACTACACG 
CTGCTAAATG TATTCTTGGC CATCGCTGTG GACAATCTGG CCAATGCCCA GGAACTGACC 
AAGGATGAGC AGGAGGAAGA AGAAGCCTTC AACCAGAAAC ATGCACTGCA GAAGGCCAAG 
GAGGTCAGCC CGATGTCTGC TCCCAACATG CCTTCCATTG AAAGAGACAG AAGGAGAAGA 
CACCACATGT CGATGTGGGA GCCACGCAGC AGCCACCTGA GGGAGCGGAG GCGCCGGCAC 
CACATGTCTG TGTGGGAACA ACGCACCAGC CAGCTGAGGA GGCACATGCA AATGTCTAGC 
CAAGAAGCCC TCAACAAAGA GGAGGCCCCG CCAATGAACC CCCTCAATCC ACTCAACCCA 
CTGAGTCCTC TCAACCCCCT CAATGCTCAC CCCAGCCTTT ATCGGAGGCC CAGGCCCATT 
GAGGGCCTAG CCCTAGGTCT GGGTCTTGAA AAGTGTGAAG AAGAACGCAT CAGTCGTGGG 
GGCTCCCTCA AGGGGGACAT AGGGGGCCTG ACTAGCGCCC TGGACAACCA GAGGAGCCCC 
TTGTCCTTGG GCAAACGGGA GCCACCGTGG CTGCCTAGAT CCTGTCATGG AAACTGTGAC 
CCAATCCAGC AGGAGGCTGG GGGAGGAGAG ACTGTGGTGA CTTTTGAGGA CCGGGCCAGG 
CACAGGCAGA GTCAGAGGCG AAGCCGGCAT CGCCGAGTCA GGACTGAGGG CAAGGACTCT 
GCCTCTGCAT CCCGAAGCAG GTCTGCCAGC CAGGAGCGGA GTCTGGATGA AGGGGTGTCC 
GTTGAGGGGG AGAAGGAACA TGAGCCTCAC AGCAGCCACA GGAGCAAAGA GCCGACCATC 
CACGAAGAGG AGAGAACCCA GGACTTAAGG AGGACCAACA GTCTGATGGT CCCCAGGGGC 
TCTGGACTGG TAGGGGCTCT TGATGAAGCA GAAACACCTC TAGTCCAGCC CCAACCTGAG 
CTGGAAGTGG GGAAGGATGC AGCTCTGACA GAGCAGGAGG CTGAAGGCAG CAGTGAACAA 
GCCCTTCTTG GGGACGTACA GCTGGATGTG GGCCGGGGCA TTAGCCAGAG TGAACCGGAT 
CTCTCCTGCA TGACAGCCAA CATGGACAAG GCTACCACGG AGAGCACCAG TGTCACGGTC 
GCCATCCCTG ATGTGGACCC CTTGGTGGAC TCGACAGTGG TGAACATTAG CAACAAGACG 
GATGGAGAAG CCAGTCCCTT GAAGGAGGCA GAGACTAAAG AGGAAGAAGA AGAGGTAGAG 
AAGAAGAAGA AGCAGAAGAA GGAGAAGCGA GAGACTGGAA AAGCCATGGT GCCCCATAGC 
TCTATGTTCA TCTTCAGCAC CACTAACCCG ATCCGCAGGG CCTGCCACTA CATTGTGAAC 
CTACGCTACT TTGAAATGTG CATTCTCTTG GTGATTGCGG CCAGCAGCAT TGCCCTGGCA 
GCCGAGGACC CTGTACTAAC TAACTCGGAG CGTAACAAAG TCCTGAGATA TTTCGACTAT 
GTTTTCACAG GAGTGTTTAC CTTTGAGATG GTTATAAAGA TGATAGACCA AGGCTTGATC 
CTGCAGGATG GATCATACTT CAGAGACCTC TGGAACATCC TGGACTTTGT AGTAGTGGTT 
GGTGCATTGG TAGCATTTGC TCTGGCGAAC GCTTTGGGAA CCAACAAGGG GAGGGATATC 
AAGACTATCA AGTCTCTGAG GGTACTTCGA GTTCTGCGGC CACTGAAAAC CATCAAGCGC 
TTGCCCAAGC TGAAGGCTGT GTTTGACTGC GTGGTGACCT CCTTGAAGAA TGTCTTCAAC 
ATACTCATTG TCTACAAGCT CTTCATGTTC ATCTTTGCCG TCATCGCTGT TCAGCTATTC 
AAAGGAAAGT TCTTTTATTG CACTGACAGT TCCAAGGACA CAGAGAAGGA GTGCATAGGC 
AACTATGTAG ACCACGAGAA AAACAAGATG GAGGTGAAGG GCCGGGAATG GAAGCGTCAT 
GAATTCCACT ATGACAACAT CATCTGGGCC CTGCTTACCC TCTTCACAGT CTCTACAGGG 
GAAGGATGGC CTCAGGTTCT GCAGCACTCT GTAGATGTGA CAGAGGAGGA CAGAGGCCCA 
AGTCGCAGCA ACCGCATGGA GATGTCCATC TTTTATGTGG TCTACTTTGT GGTCTTCCCT 
TTCTTCTTTG TCAATATCTT CGTGGCTCTC ATTATCATCA CCTTCCAGGA GCAAGGGGAC 
AAGATGATGG AGGAGTGTAG CCTGGAGAAG AATGAGCGGG CATGCATCGA CTTCGCCATT 
AGTGCCAAAC CTCTCACCCG CTACATGCCA CAGAACAGGC ACACCTTCCA GTACCGTGTG 
TGGCACTTTG TAGTCTCCCC ATCCTTTGAG TATACCATCA TGGCCATGAT TGCCTTGAAC 
ACTGTCGTGC TGATGATGAA GTACTACTCT GCTCCTTGCA CCTATGAGCT CGCCTTAAAG 
TACCTGAACA TCGCCTTCAC TATGGTGTTT TCCCTGGAGT GTGTCCTAAA GGTCATTGCT 
TTCGGCTTCC TGAACTATTT CCGAGATACC TGGAACATCT TTGACTTCAT CACGGTGATC 
GGCAGTATCA CAGAAATTAT CCTGACAGAT AGCAAGCTGG TGAACACCAG TGGCTTCAAT 
ATGAGCTTTC TGAAGCTCTT CCGAGCTGCA CGGCTCATAA AGCTTCTGAG ACAGGGCTAC 
ACCATCCGCA TCTTACTGTG GACCTTCGTG CAGTCCTTTA AGGCTCTTCC CTACGTCTGC 
CTTCTCATTG CCATGCTTTT CTTCATCTAC GCCATCATTG GGATGCAGGT GTTTGGAAAC 
ATAAAGTTAG ATGAGGAGAG TCACATCAAC CGGCACAACA ACTTCCGGAG TTTCTTTGGA 
TCCCTCATGC TGCTTTTCAG GAGTGCCACG GGGGAGGCTT GGCAGGAGAT TATGCTGTCC 
TGTCTCGGTG AGAAGGGTTG TGAGCCTGAC ACCACTGCAC CCTCAGGGCA GAATGAGAGT 
GAGCGCTGTG GCACTGACCT GGCCTACGTT TATTTTGTCT CCTTCATCTT CTTCTGCTCC 
TTCCTGATGC TCAACCTGTT CGTGGCTGTC ATCATGGACA ACTTTGAGTA CCTGACTCGA 
GATTCCTCCA TCCTGGGGCC TCACCACTTA GATGAGTTTG TCCGTGTCTG GGCAGAATAT 
GACAGAGCAG CATGTGGCCG CATCCATTAC ACTGAGATGT ATGAAATGCT GACTCTCATG 
TCACCACCGC TAGGCCTCGG CAAGAGATGT CCCTCCAAAG TGGCCTATAA GAGGCTGGTC 
CTGATGAACA TGCCAGTGGC TGAGGACATG ACAGTCCACT TCACCTCCAC ACTTATGGCT 
CTGATCCGGA CAGCTCTGGA CATTAAAATC GCCAAAGGTG GTGCAGACAG ACAGCAGCTA 
GACTCGGAGC TGCAAAAAGA GACCCTGGCC ATTTGGCCTC ACCTGTCCCA AAAGATGTTG 
GATCTGCTTG TGCCCATGCC CAAAGCCTCT GACCTGACTG TGGGCAAGAT CTATGCAGCA 
ATGATGATCA TGGACTACTA TAAACAGAGT AAGGTAAAGA AACAGAGACA GCAGCTGGAA 
GAACAGAAAA ATGCACCCAT GTTCCAGCGC ATGGAGCCCT CATCACTGCC TCAGGAGATC 
ATTGCTAATG CCAAAGCCCT GCCTTATCTC CAGCAGGACC CTGTTTCTGG CCTGAGTGGT 
CGGAGTGGAT ACCCTTCAAT GAGTCCACTC TCCCCTCAAG AAATATTCCA GTTGGCTTGT 
ATGGACCCAG CGGATGATGG ACAATTCCAG GAACAACAAT CTCTGGTGAA TGCACAAGAG 
CCAGAGGTTA GTGAATTAAA AAGTGTGCAG TCCTCTAACC ATGGCATCTA CCTTCCTCCG 
GACACCCAGG AGCATGCGGG ATCTGGGAGG GCATCCTCTA TGCCACGTCT GACTATGGAT 
CCCCAGGTGG TAACAGACCC TAGCTCTATG CGACGTTCAT TTTCTACAAT TCGGGATAAG 
CGTTCAAATT CCTCCTGGTT GGAGGAGTTC TCCATGGAAC GCAGCAGTGA AAACACTTAC 
AAGTCGCGGC GCAGAAGTTA CCATTCCTCC CTGAGGCTGT CAGCACACCG CCTGAATTCT 
GACTCTGGTC ACAAGTCTGA CACTCACCGA TCAGGAGGCA GGGAGAGAGG CCGATCCAAA 
GAACGAAAGC ACCTCCTCTC TCCAGATGTC TCCCGCTGCA ATTCTGAAGA GCGAGGGACC 
CAGGCCGACT GGGAGTCCCC AGAACGTCGC CAGTCCAGGT CACCCAGTGA AGGAAGGTCA 
CAGACCCCCA ATAGACAGGG TACCGGTTCC CTAAGTGAGA GCTCCATCCC CTCTATTTCC 
GACACCAGCA CCCCAAGACG AAGCCGTCGC CAGCTCCCAC CTGTGCCACC AAAGCCTCGT 
CCCCTCCTCT CCTACAGCTC CCTGATGAGA CACACTGGCG GCATCTCTCC ACCTCCTGAT 
GGAAGTGAGG GTGGATCCCC ACTGGCCTCT CAAGCTCTGG AGAGCAACAG CGCTTGCCTG 
ACCGAGTCTT CCAACTCCTT GCACCCCCAG CAGGGCCAGC ATCCTTCCCC ACAGCACTAC 
ATCTCCGAGC CCTATCTGGC CCTCCATGAA GACTCCCACG CCTCAGACTG TGGCGAGGAG 
GAGACACTCA CCTTTGAGGC AGCCGTAGCC ACTAGCTTGG GCCGGTCCAA TACCATTGGC 
TCTGCGCCAC CCCTACGGCA CAGCTGGCAG ATGCCTAATG GGCACTATCG GCGGCGGAGG 
CGTGGGGGGC CTGGGCCTGG CATGATGTGT GGAGCTGTCA GTGACCTCCT GAGTGACACG 
GAAGAAGACG ATAAGTGCTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030099786.1
CDS	670..8070
Translation	MVPEMRGLSPGRRLPEPTLCPATGGSPEPGAAGRRRHVLALPDEEAWRPQQCALLEANASDEVGMLPASPRSAVAGFDNFFPVQEGEGPGWEGSTTLDAGSSPFLPVSPEVMKRRRGGLIEQRDIIKAHEAHKMQSTPQARRKEWEMARFGEAVVVGRPGSGDGDSDQSRNRQGTPVPASGPAAAYKQSKAQRARTMALYNPIPVRQNCFTVNRSLFIFGEDNIVRKYAKKLIDWPPFEYMILATIIANCIVLALEQHLPEDDKTPMSRRLEKTEPYFIGIFCFEAGIKIVALGFIFHKGSYLRNGWNVMDFIVVLSGILATAGTHFNTHVDLRTLRAVRVLRPLKLVSGIPSLQIVLKSIMKAMVPLLQIGLLLFFAILMFAIIGLEFYSGKLHRACFMNNSGILEGFDPPHPCGVQGCPAGYECKDWIGPNDGITQFDNILFAVLTVFQCITMEGWTTVLYNTNDALGATWNWLYFIPLIIIGSFFVLNLVLGVLSGEFAKERERVENRRAFMKLRRQQQIERELNGYRAWIDKAEEVMLAEENKNSGTSALEVLRRATIKRSRTEAMTRDSSDEHCVDISSVGTPLARASIKSTKVDGASYFRHKERLLRISIRHMVKSQVFYWIVLSVVALNTACVAIVHHNQPQWLTHLLYYAEFLFLGLFLLEMSLKMYGMGPRLYFHSSFNCFDFGVTVGSIFEVVWAIFRPGTSFGISVLRALRLLRIFKITKYWASLRNLVVSLMSSMKSIISLLFLLFLFIVVFALLGMQLFGGRFNFNDGTPSANFDTFPAAIMTVFQILTGEDWNEVMYNGIRSQGGVSSGMWSAIYFIVLTLFGNYTLLNVFLAIAVDNLANAQELTKDEQEEEEAFNQKHALQKAKEVSPMSAPNMPSIERDRRRRHHMSMWEPRSSHLRERRRRHHMSVWEQRTSQLRRHMQMSSQEALNKEEAPPMNPLNPLNPLSPLNPLNAHPSLYRRPRPIEGLALGLGLEKCEEERISRGGSLKGDIGGLTSALDNQRSPLSLGKREPPWLPRSCHGNCDPIQQEAGGGETVVTFEDRARHRQSQRRSRHRRVRTEGKDSASASRSRSASQERSLDEGVSVEGEKEHEPHSSHRSKEPTIHEEERTQDLRRTNSLMVPRGSGLVGALDEAETPLVQPQPELEVGKDAALTEQEAEGSSEQALLGDVQLDVGRGISQSEPDLSCMTANMDKATTESTSVTVAIPDVDPLVDSTVVNISNKTDGEASPLKEAETKEEEEEVEKKKKQKKEKRETGKAMVPHSSMFIFSTTNPIRRACHYIVNLRYFEMCILLVIAASSIALAAEDPVLTNSERNKVLRYFDYVFTGVFTFEMVIKMIDQGLILQDGSYFRDLWNILDFVVVVGALVAFALANALGTNKGRDIKTIKSLRVLRVLRPLKTIKRLPKLKAVFDCVVTSLKNVFNILIVYKLFMFIFAVIAVQLFKGKFFYCTDSSKDTEKECIGNYVDHEKNKMEVKGREWKRHEFHYDNIIWALLTLFTVSTGEGWPQVLQHSVDVTEEDRGPSRSNRMEMSIFYVVYFVVFPFFFVNIFVALIIITFQEQGDKMMEECSLEKNERACIDFAISAKPLTRYMPQNRHTFQYRVWHFVVSPSFEYTIMAMIALNTVVLMMKYYSAPCTYELALKYLNIAFTMVFSLECVLKVIAFGFLNYFRDTWNIFDFITVIGSITEIILTDSKLVNTSGFNMSFLKLFRAARLIKLLRQGYTIRILLWTFVQSFKALPYVCLLIAMLFFIYAIIGMQVFGNIKLDEESHINRHNNFRSFFGSLMLLFRSATGEAWQEIMLSCLGEKGCEPDTTAPSGQNESERCGTDLAYVYFVSFIFFCSFLMLNLFVAVIMDNFEYLTRDSSILGPHHLDEFVRVWAEYDRAACGRIHYTEMYEMLTLMSPPLGLGKRCPSKVAYKRLVLMNMPVAEDMTVHFTSTLMALIRTALDIKIAKGGADRQQLDSELQKETLAIWPHLSQKMLDLLVPMPKASDLTVGKIYAAMMIMDYYKQSKVKKQRQQLEEQKNAPMFQRMEPSSLPQEIIANAKALPYLQQDPVSGLSGRSGYPSMSPLSPQEIFQLACMDPADDGQFQEQQSLVNAQEPEVSELKSVQSSNHGIYLPPDTQEHAGSGRASSMPRLTMDPQVVTDPSSMRRSFSTIRDKRSNSSWLEEFSMERSSENTYKSRRRSYHSSLRLSAHRLNSDSGHKSDTHRSGGRERGRSKERKHLLSPDVSRCNSEERGTQADWESPERRQSRSPSEGRSQTPNRQGTGSLSESSIPSISDTSTPRRSRRQLPPVPPKPRPLLSYSSLMRHTGGISPPPDGSEGGSPLASQALESNSACLTESSNSLHPQQGQHPSPQHYISEPYLALHEDSHASDCGEEETLTFEAAVATSLGRSNTIGSAPPLRHSWQMPNGHYRRRRRGGPGPGMMCGAVSDLLSDTEEDDKC

Target ORF information:

RefSeq Version	XM_030243926.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus calcium channel, voltage-dependent, R type, alpha 1E subunit (Cacna1e), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030243926.1

ORF Insert Sequence:

ATGGTGCCCG AGATGCGTGG ACTGAGCCCA GGCCGAAGGC TGCCCGAGCC CACCCTGTGC 
CCGGCTACAG GAGGGAGCCC TGAGCCCGGG GCTGCTGGCA GGCGCCGCCA CGTCCTGGCT 
CTGCCTGATG AGGAGGCATG GCGGCCGCAG CAGTGCGCCC TGCTCGAGGC CAATGCCTCC 
GACGAGGTGG GCATGTTGCC TGCTTCCCCG CGCTCCGCGG TGGCCGGCTT CGACAACTTC 
TTCCCGGTGC AGGAAGGCGA GGGCCCGGGC TGGGAGGGCA GCACCACGCT GGATGCAGGC 
TCCAGCCCCT TCCTTCCTGT GAGCCCAGAG GTCATGAAGC GGAGGCGAGG GGGGCTCATC 
GAGCAGCGGG ACATCATCAA GGCCCACGAG GCACACAAGA TGCAGAGCAC CCCGCAGGCC 
CGGCGCAAGG AGTGGGAGAT GGCTCGCTTC GGGGAGGCGG TGGTCGTTGG CAGGCCAGGC 
TCAGGCGATG GAGACTCGGA CCAGAGCAGG AACCGACAAG GAACCCCCGT CCCGGCCTCG 
GGGCCGGCGG CCGCCTACAA GCAGTCAAAA GCGCAGAGGG CGCGGACTAT GGCTTTGTAC 
AACCCCATAC CAGTCCGGCA GAACTGTTTC ACGGTCAACA GATCCCTGTT CATCTTCGGA 
GAAGATAACA TTGTCAGGAA ATACGCCAAG AAGCTCATCG ATTGGCCGCC ATTTGAGTAC 
ATGATCCTGG CCACCATCAT TGCCAACTGC ATCGTCTTGG CCCTGGAGCA GCATCTTCCT 
GAGGATGACA AGACCCCAAT GTCTCGAAGA CTGGAGAAGA CAGAACCATA TTTCATTGGG 
ATCTTCTGCT TTGAAGCTGG GATCAAAATT GTGGCTCTAG GGTTCATCTT CCATAAGGGT 
TCATACCTCC GTAATGGCTG GAATGTCATG GACTTCATCG TGGTCCTTAG TGGCATCCTG 
GCCACTGCGG GAACCCACTT CAACACCCAC GTGGACCTGC GGACCCTCCG GGCTGTGCGT 
GTCCTGAGGC CTTTAAAGCT GGTATCAGGA ATACCTAGCC TGCAGATCGT GCTGAAGTCT 
ATCATGAAGG CCATGGTGCC TCTTCTGCAG ATTGGCCTTC TGCTCTTCTT TGCCATCCTC 
ATGTTTGCTA TCATTGGCTT GGAGTTCTAC AGTGGCAAGT TGCATCGAGC ATGCTTCATG 
AACAATTCAG GTATTCTAGA GGGGTTTGAT CCTCCTCACC CGTGTGGTGT GCAAGGCTGC 
CCGGCTGGTT ATGAATGTAA GGACTGGATC GGCCCCAATG ACGGGATCAC CCAGTTTGAC 
AACATCCTTT TTGCCGTGCT GACTGTCTTC CAATGCATCA CTATGGAAGG GTGGACCACT 
GTGCTGTACA ATACCAATGA TGCCTTAGGA GCCACCTGGA ACTGGCTGTA CTTCATCCCT 
CTCATCATCA TCGGATCCTT CTTTGTTCTC AACCTCGTCC TGGGAGTGCT TTCCGGGGAA 
TTTGCCAAAG AAAGAGAGAG AGTGGAGAAC CGAAGAGCGT TCATGAAGCT CCGGCGCCAG 
CAACAGATTG AGCGAGAATT GAACGGCTAC CGTGCCTGGA TAGACAAAGC AGAGGAAGTC 
ATGCTTGCTG AAGAAAATAA AAACTCGGGA ACATCAGCCT TGGAAGTGCT TCGAAGGGCA 
ACCATCAAAA GGAGCCGGAC GGAGGCCATG ACCCGAGACT CCAGCGATGA GCACTGCGTT 
GATATCTCTT CAGTGGGCAC ACCTCTTGCC AGAGCCAGTA TCAAGAGCAC AAAAGTAGAT 
GGGGCCTCCT ATTTCCGGCA CAAGGAACGG CTTCTGCGCA TCTCTATCCG CCACATGGTC 
AAGTCCCAAG TCTTTTACTG GATCGTCCTG AGTGTTGTGG CTCTTAATAC TGCCTGTGTG 
GCCATTGTTC ATCACAACCA GCCACAGTGG CTCACTCACC TCCTCTACTA TGCAGAGTTT 
TTGTTTCTGG GACTCTTCCT GTTGGAGATG TCCCTGAAGA TGTATGGCAT GGGGCCACGC 
CTCTATTTTC ACTCATCCTT CAACTGCTTT GATTTTGGGG TCACAGTGGG CAGCATCTTT 
GAGGTTGTCT GGGCAATCTT CAGACCTGGC ACATCTTTTG GAATCAGTGT CTTACGAGCT 
CTCCGGCTCC TAAGGATATT TAAAATAACC AAGTATTGGG CATCCCTCCG GAATCTGGTT 
GTCTCCTTGA TGAGCTCCAT GAAGTCTATC ATCAGCTTAC TCTTCCTTCT CTTCCTCTTC 
ATCGTTGTCT TTGCTCTCCT GGGAATGCAG CTCTTTGGAG GCAGGTTTAA CTTTAATGAT 
GGGACTCCTT CAGCTAATTT TGACACCTTC CCTGCAGCTA TCATGACAGT GTTCCAGATC 
CTGACAGGGG AAGACTGGAA TGAGGTGATG TACAATGGCA TTCGCTCCCA GGGTGGGGTC 
AGCTCAGGCA TGTGGTCTGC CATCTACTTC ATTGTGCTCA CCTTGTTTGG AAACTACACG 
CTGCTAAATG TATTCTTGGC CATCGCTGTG GACAATCTGG CCAATGCCCA GGAACTGACC 
AAGGATGAGC AGGAGGAAGA AGAAGCCTTC AACCAGAAAC ATGCACTGCA GAAGGCCAAG 
GAGGTCAGCC CGATGTCTGC TCCCAACATG CCTTCCATTG AAAGAGACAG AAGGAGAAGA 
CACCACATGT CGATGTGGGA GCCACGCAGC AGCCACCTGA GGGAGCGGAG GCGCCGGCAC 
CACATGTCTG TGTGGGAACA ACGCACCAGC CAGCTGAGGA GGCACATGCA AATGTCTAGC 
CAAGAAGCCC TCAACAAAGA GGAGGCCCCG CCAATGAACC CCCTCAATCC ACTCAACCCA 
CTGAGTCCTC TCAACCCCCT CAATGCTCAC CCCAGCCTTT ATCGGAGGCC CAGGCCCATT 
GAGGGCCTAG CCCTAGGTCT GGGTCTTGAA AAGTGTGAAG AAGAACGCAT CAGTCGTGGG 
GGCTCCCTCA AGGGGGACAT AGGGGGCCTG ACTAGCGCCC TGGACAACCA GAGGAGCCCC 
TTGTCCTTGG GCAAACGGGA GCCACCGTGG CTGCCTAGAT CCTGTCATGG AAACTGTGAC 
CCAATCCAGC AGGAGGCTGG GGGAGGAGAG ACTGTGGTGA CTTTTGAGGA CCGGGCCAGG 
CACAGGCAGA GTCAGAGGCG AAGCCGGCAT CGCCGAGTCA GGACTGAGGG CAAGGACTCT 
GCCTCTGCAT CCCGAAGCAG GTCTGCCAGC CAGGAGCGGA GTCTGGATGA AGGGGTGTCC 
GTTGAGGGGG AGAAGGAACA TGAGCCTCAC AGCAGCCACA GGAGCAAAGA GCCGACCATC 
CACGAAGAGG AGAGAACCCA GGACTTAAGG AGGACCAACA GTCTGATGGT CCCCAGGGGC 
TCTGGACTGG TAGGGGCTCT TGATGAAGCA GAAACACCTC TAGTCCAGCC CCAACCTGAG 
CTGGAAGTGG GGAAGGATGC AGCTCTGACA GAGCAGGAGG CTGAAGGCAG CAGTGAACAA 
GCCCTTCTTG GGGACGTACA GCTGGATGTG GGCCGGGGCA TTAGCCAGAG TGAACCGGAT 
CTCTCCTGCA TGACAGCCAA CATGGACAAG GCTACCACGG AGAGCACCAG TGTCACGGTC 
GCCATCCCTG ATGTGGACCC CTTGGTGGAC TCGACAGTGG TGAACATTAG CAACAAGACG 
GATGGAGAAG CCAGTCCCTT GAAGGAGGCA GAGACTAAAG AGGAAGAAGA AGAGGTAGAG 
AAGAAGAAGA AGCAGAAGAA GGAGAAGCGA GAGACTGGAA AAGCCATGGT GCCCCATAGC 
TCTATGTTCA TCTTCAGCAC CACTAACCCG ATCCGCAGGG CCTGCCACTA CATTGTGAAC 
CTACGCTACT TTGAAATGTG CATTCTCTTG GTGATTGCGG CCAGCAGCAT TGCCCTGGCA 
GCCGAGGACC CTGTACTAAC TAACTCGGAG CGTAACAAAG TCCTGAGATA TTTCGACTAT 
GTTTTCACAG GAGTGTTTAC CTTTGAGATG GTTATAAAGA TGATAGACCA AGGCTTGATC 
CTGCAGGATG GATCATACTT CAGAGACCTC TGGAACATCC TGGACTTTGT AGTAGTGGTT 
GGTGCATTGG TAGCATTTGC TCTGGCGAAC GCTTTGGGAA CCAACAAGGG GAGGGATATC 
AAGACTATCA AGTCTCTGAG GGTACTTCGA GTTCTGCGGC CACTGAAAAC CATCAAGCGC 
TTGCCCAAGC TGAAGGCTGT GTTTGACTGC GTGGTGACCT CCTTGAAGAA TGTCTTCAAC 
ATACTCATTG TCTACAAGCT CTTCATGTTC ATCTTTGCCG TCATCGCTGT TCAGCTATTC 
AAAGGAAAGT TCTTTTATTG CACTGACAGT TCCAAGGACA CAGAGAAGGA GTGCATAGGC 
AACTATGTAG ACCACGAGAA AAACAAGATG GAGGTGAAGG GCCGGGAATG GAAGCGTCAT 
GAATTCCACT ATGACAACAT CATCTGGGCC CTGCTTACCC TCTTCACAGT CTCTACAGGG 
GAAGGATGGC CTCAGGTTCT GCAGCACTCT GTAGATGTGA CAGAGGAGGA CAGAGGCCCA 
AGTCGCAGCA ACCGCATGGA GATGTCCATC TTTTATGTGG TCTACTTTGT GGTCTTCCCT 
TTCTTCTTTG TCAATATCTT CGTGGCTCTC ATTATCATCA CCTTCCAGGA GCAAGGGGAC 
AAGATGATGG AGGAGTGTAG CCTGGAGAAG AATGAGCGGG CATGCATCGA CTTCGCCATT 
AGTGCCAAAC CTCTCACCCG CTACATGCCA CAGAACAGGC ACACCTTCCA GTACCGTGTG 
TGGCACTTTG TAGTCTCCCC ATCCTTTGAG TATACCATCA TGGCCATGAT TGCCTTGAAC 
ACTGTCGTGC TGATGATGAA GTACTACTCT GCTCCTTGCA CCTATGAGCT CGCCTTAAAG 
TACCTGAACA TCGCCTTCAC TATGGTGTTT TCCCTGGAGT GTGTCCTAAA GGTCATTGCT 
TTCGGCTTCC TGAACTATTT CCGAGATACC TGGAACATCT TTGACTTCAT CACGGTGATC 
GGCAGTATCA CAGAAATTAT CCTGACAGAT AGCAAGCTGG TGAACACCAG TGGCTTCAAT 
ATGAGCTTTC TGAAGCTCTT CCGAGCTGCA CGGCTCATAA AGCTTCTGAG ACAGGGCTAC 
ACCATCCGCA TCTTACTGTG GACCTTCGTG CAGTCCTTTA AGGCTCTTCC CTACGTCTGC 
CTTCTCATTG CCATGCTTTT CTTCATCTAC GCCATCATTG GGATGCAGGT GTTTGGAAAC 
ATAAAGTTAG ATGAGGAGAG TCACATCAAC CGGCACAACA ACTTCCGGAG TTTCTTTGGA 
TCCCTCATGC TGCTTTTCAG GAGTGCCACG GGGGAGGCTT GGCAGGAGAT TATGCTGTCC 
TGTCTCGGTG AGAAGGGTTG TGAGCCTGAC ACCACTGCAC CCTCAGGGCA GAATGAGAGT 
GAGCGCTGTG GCACTGACCT GGCCTACGTT TATTTTGTCT CCTTCATCTT CTTCTGCTCC 
TTCCTGATGC TCAACCTGTT CGTGGCTGTC ATCATGGACA ACTTTGAGTA CCTGACTCGA 
GATTCCTCCA TCCTGGGGCC TCACCACTTA GATGAGTTTG TCCGTGTCTG GGCAGAATAT 
GACAGAGCAG CATGTGGCCG CATCCATTAC ACTGAGATGT ATGAAATGCT GACTCTCATG 
TCACCACCGC TAGGCCTCGG CAAGAGATGT CCCTCCAAAG TGGCCTATAA GAGGCTGGTC 
CTGATGAACA TGCCAGTGGC TGAGGACATG ACAGTCCACT TCACCTCCAC ACTTATGGCT 
CTGATCCGGA CAGCTCTGGA CATTAAAATC GCCAAAGGTG GTGCAGACAG ACAGCAGCTA 
GACTCGGAGC TGCAAAAAGA GACCCTGGCC ATTTGGCCTC ACCTGTCCCA AAAGATGTTG 
GATCTGCTTG TGCCCATGCC CAAAGCCTCT GACCTGACTG TGGGCAAGAT CTATGCAGCA 
ATGATGATCA TGGACTACTA TAAACAGAGT AAGGTAAAGA AACAGAGACA GCAGCTGGAA 
GAACAGAAAA ATGCACCCAT GTTCCAGCGC ATGGAGCCCT CATCACTGCC TCAGGAGATC 
ATTGCTAATG CCAAAGCCCT GCCTTATCTC CAGCAGGACC CTGTTTCTGG CCTGAGTGGT 
CGGAGTGGAT ACCCTTCAAT GAGTCCACTC TCCCCTCAAG AAATATTCCA GTTGGCTTGT 
ATGGACCCAG CGGATGATGG ACAATTCCAG GAACAACAAT CTCTGGTGAA TGCACAAGAG 
CCAGAGGTTA GTGAATTAAA AAGTGTGCAG TCCTCTAACC ATGGCATCTA CCTTCCTCCG 
GACACCCAGG AGCATGCGGG ATCTGGGAGG GCATCCTCTA TGCCACGTCT GACTATGGAT 
CCCCAGGTGG TAACAGACCC TAGCTCTATG CGACGTTCAT TTTCTACAAT TCGGGATAAG 
CGTTCAAATT CCTCCTGGTT GGAGGAGTTC TCCATGGAAC GCAGCAGTGA AAACACTTAC 
AAGTCGCGGC GCAGAAGTTA CCATTCCTCC CTGAGGCTGT CAGCACACCG CCTGAATTCT 
GACTCTGGTC ACAAGTCTGA CACTCACCGA TCAGGAGGCA GGGAGAGAGG CCGATCCAAA 
GAACGAAAGC ACCTCCTCTC TCCAGATGTC TCCCGCTGCA ATTCTGAAGA GCGAGGGACC 
CAGGCCGACT GGGAGTCCCC AGAACGTCGC CAGTCCAGGT CACCCAGTGA AGGAAGGTCA 
CAGACCCCCA ATAGACAGGG TACCGGTTCC CTAAGTGAGA GCTCCATCCC CTCTATTTCC 
GACACCAGCA CCCCAAGACG AAGCCGTCGC CAGCTCCCAC CTGTGCCACC AAAGCCTCGT 
CCCCTCCTCT CCTACAGCTC CCTGATGAGA CACACTGGCG GCATCTCTCC ACCTCCTGAT 
GGAAGTGAGG GTGGATCCCC ACTGGCCTCT CAAGCTCTGG AGAGCAACAG CGCTTGCCTG 
ACCGAGTCTT CCAACTCCTT GCACCCCCAG CAGGGCCAGC ATCCTTCCCC ACAGCACTAC 
ATCTCCGAGC CCTATCTGGC CCTCCATGAA GACTCCCACG CCTCAGACTG TGGCGAGGAG 
GAGACACTCA CCTTTGAGGC AGCCGTAGCC ACTAGCTTGG GCCGGTCCAA TACCATTGGC 
TCTGCGCCAC CCCTACGGCA CAGCTGGCAG ATGCCTAATG GGCACTATCG GCGGCGGAGG 
CGTGGGGGGC CTGGGCCTGG CATGATGTGT GGAGCTGTCA GTGACCTCCT GAGTGACACG 
GAAGAAGACG ATAAGTGCTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cacna1e ( XM_030243929.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030099789.1 Mus musculus calcium channel, voltage-dependent, R type, alpha 1E subunit (Cacna1e), transcript variant X4, mRNA.

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CloneID	OMu67260
Clone ID Related Accession (Same CDS sequence)	XM_017312578.1 , XM_030243926.1 , XM_017312594.1 , XM_030243929.1 , XM_011247909.2 , XM_017312578.2 , XM_011247909.3
Accession Version	XM_030243929.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7401bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	voltage-dependent R-type calcium channel subunit alpha-1E isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGCCCG AGATGCGTGG ACTGAGCCCA GGCCGAAGGC TGCCCGAGCC CACCCTGTGC 
CCGGCTACAG GAGGGAGCCC TGAGCCCGGG GCTGCTGGCA GGCGCCGCCA CGTCCTGGCT 
CTGCCTGATG AGGAGGCATG GCGGCCGCAG CAGTGCGCCC TGCTCGAGGC CAATGCCTCC 
GACGAGGTGG GCATGTTGCC TGCTTCCCCG CGCTCCGCGG TGGCCGGCTT CGACAACTTC 
TTCCCGGTGC AGGAAGGCGA GGGCCCGGGC TGGGAGGGCA GCACCACGCT GGATGCAGGC 
TCCAGCCCCT TCCTTCCTGT GAGCCCAGAG GTCATGAAGC GGAGGCGAGG GGGGCTCATC 
GAGCAGCGGG ACATCATCAA GGCCCACGAG GCACACAAGA TGCAGAGCAC CCCGCAGGCC 
CGGCGCAAGG AGTGGGAGAT GGCTCGCTTC GGGGAGGCGG TGGTCGTTGG CAGGCCAGGC 
TCAGGCGATG GAGACTCGGA CCAGAGCAGG AACCGACAAG GAACCCCCGT CCCGGCCTCG 
GGGCCGGCGG CCGCCTACAA GCAGTCAAAA GCGCAGAGGG CGCGGACTAT GGCTTTGTAC 
AACCCCATAC CAGTCCGGCA GAACTGTTTC ACGGTCAACA GATCCCTGTT CATCTTCGGA 
GAAGATAACA TTGTCAGGAA ATACGCCAAG AAGCTCATCG ATTGGCCGCC ATTTGAGTAC 
ATGATCCTGG CCACCATCAT TGCCAACTGC ATCGTCTTGG CCCTGGAGCA GCATCTTCCT 
GAGGATGACA AGACCCCAAT GTCTCGAAGA CTGGAGAAGA CAGAACCATA TTTCATTGGG 
ATCTTCTGCT TTGAAGCTGG GATCAAAATT GTGGCTCTAG GGTTCATCTT CCATAAGGGT 
TCATACCTCC GTAATGGCTG GAATGTCATG GACTTCATCG TGGTCCTTAG TGGCATCCTG 
GCCACTGCGG GAACCCACTT CAACACCCAC GTGGACCTGC GGACCCTCCG GGCTGTGCGT 
GTCCTGAGGC CTTTAAAGCT GGTATCAGGA ATACCTAGCC TGCAGATCGT GCTGAAGTCT 
ATCATGAAGG CCATGGTGCC TCTTCTGCAG ATTGGCCTTC TGCTCTTCTT TGCCATCCTC 
ATGTTTGCTA TCATTGGCTT GGAGTTCTAC AGTGGCAAGT TGCATCGAGC ATGCTTCATG 
AACAATTCAG GTATTCTAGA GGGGTTTGAT CCTCCTCACC CGTGTGGTGT GCAAGGCTGC 
CCGGCTGGTT ATGAATGTAA GGACTGGATC GGCCCCAATG ACGGGATCAC CCAGTTTGAC 
AACATCCTTT TTGCCGTGCT GACTGTCTTC CAATGCATCA CTATGGAAGG GTGGACCACT 
GTGCTGTACA ATACCAATGA TGCCTTAGGA GCCACCTGGA ACTGGCTGTA CTTCATCCCT 
CTCATCATCA TCGGATCCTT CTTTGTTCTC AACCTCGTCC TGGGAGTGCT TTCCGGGGAA 
TTTGCCAAAG AAAGAGAGAG AGTGGAGAAC CGAAGAGCGT TCATGAAGCT CCGGCGCCAG 
CAACAGATTG AGCGAGAATT GAACGGCTAC CGTGCCTGGA TAGACAAAGC AGAGGAAGTC 
ATGCTTGCTG AAGAAAATAA AAACTCGGGA ACATCAGCCT TGGAAGTGCT TCGAAGGGCA 
ACCATCAAAA GGAGCCGGAC GGAGGCCATG ACCCGAGACT CCAGCGATGA GCACTGCGTT 
GATATCTCTT CAGTGGGCAC ACCTCTTGCC AGAGCCAGTA TCAAGAGCAC AAAAGTAGAT 
GGGGCCTCCT ATTTCCGGCA CAAGGAACGG CTTCTGCGCA TCTCTATCCG CCACATGGTC 
AAGTCCCAAG TCTTTTACTG GATCGTCCTG AGTGTTGTGG CTCTTAATAC TGCCTGTGTG 
GCCATTGTTC ATCACAACCA GCCACAGTGG CTCACTCACC TCCTCTACTA TGCAGAGTTT 
TTGTTTCTGG GACTCTTCCT GTTGGAGATG TCCCTGAAGA TGTATGGCAT GGGGCCACGC 
CTCTATTTTC ACTCATCCTT CAACTGCTTT GATTTTGGGG TCACAGTGGG CAGCATCTTT 
GAGGTTGTCT GGGCAATCTT CAGACCTGGC ACATCTTTTG GAATCAGTGT CTTACGAGCT 
CTCCGGCTCC TAAGGATATT TAAAATAACC AAGTATTGGG CATCCCTCCG GAATCTGGTT 
GTCTCCTTGA TGAGCTCCAT GAAGTCTATC ATCAGCTTAC TCTTCCTTCT CTTCCTCTTC 
ATCGTTGTCT TTGCTCTCCT GGGAATGCAG CTCTTTGGAG GCAGGTTTAA CTTTAATGAT 
GGGACTCCTT CAGCTAATTT TGACACCTTC CCTGCAGCTA TCATGACAGT GTTCCAGATC 
CTGACAGGGG AAGACTGGAA TGAGGTGATG TACAATGGCA TTCGCTCCCA GGGTGGGGTC 
AGCTCAGGCA TGTGGTCTGC CATCTACTTC ATTGTGCTCA CCTTGTTTGG AAACTACACG 
CTGCTAAATG TATTCTTGGC CATCGCTGTG GACAATCTGG CCAATGCCCA GGAACTGACC 
AAGGATGAGC AGGAGGAAGA AGAAGCCTTC AACCAGAAAC ATGCACTGCA GAAGGCCAAG 
GAGGTCAGCC CGATGTCTGC TCCCAACATG CCTTCCATTG AAAGAGACAG AAGGAGAAGA 
CACCACATGT CGATGTGGGA GCCACGCAGC AGCCACCTGA GGGAGCGGAG GCGCCGGCAC 
CACATGTCTG TGTGGGAACA ACGCACCAGC CAGCTGAGGA GGCACATGCA AATGTCTAGC 
CAAGAAGCCC TCAACAAAGA GGAGGCCCCG CCAATGAACC CCCTCAATCC ACTCAACCCA 
CTGAGTCCTC TCAACCCCCT CAATGCTCAC CCCAGCCTTT ATCGGAGGCC CAGGCCCATT 
GAGGGCCTAG CCCTAGGTCT GGGTCTTGAA AAGTGTGAAG AAGAACGCAT CAGTCGTGGG 
GGCTCCCTCA AGGGGGACAT AGGGGGCCTG ACTAGCGCCC TGGACAACCA GAGGAGCCCC 
TTGTCCTTGG GCAAACGGGA GCCACCGTGG CTGCCTAGAT CCTGTCATGG AAACTGTGAC 
CCAATCCAGC AGGAGGCTGG GGGAGGAGAG ACTGTGGTGA CTTTTGAGGA CCGGGCCAGG 
CACAGGCAGA GTCAGAGGCG AAGCCGGCAT CGCCGAGTCA GGACTGAGGG CAAGGACTCT 
GCCTCTGCAT CCCGAAGCAG GTCTGCCAGC CAGGAGCGGA GTCTGGATGA AGGGGTGTCC 
GTTGAGGGGG AGAAGGAACA TGAGCCTCAC AGCAGCCACA GGAGCAAAGA GCCGACCATC 
CACGAAGAGG AGAGAACCCA GGACTTAAGG AGGACCAACA GTCTGATGGT CCCCAGGGGC 
TCTGGACTGG TAGGGGCTCT TGATGAAGCA GAAACACCTC TAGTCCAGCC CCAACCTGAG 
CTGGAAGTGG GGAAGGATGC AGCTCTGACA GAGCAGGAGG CTGAAGGCAG CAGTGAACAA 
GCCCTTCTTG GGGACGTACA GCTGGATGTG GGCCGGGGCA TTAGCCAGAG TGAACCGGAT 
CTCTCCTGCA TGACAGCCAA CATGGACAAG GCTACCACGG AGAGCACCAG TGTCACGGTC 
GCCATCCCTG ATGTGGACCC CTTGGTGGAC TCGACAGTGG TGAACATTAG CAACAAGACG 
GATGGAGAAG CCAGTCCCTT GAAGGAGGCA GAGACTAAAG AGGAAGAAGA AGAGGTAGAG 
AAGAAGAAGA AGCAGAAGAA GGAGAAGCGA GAGACTGGAA AAGCCATGGT GCCCCATAGC 
TCTATGTTCA TCTTCAGCAC CACTAACCCG ATCCGCAGGG CCTGCCACTA CATTGTGAAC 
CTACGCTACT TTGAAATGTG CATTCTCTTG GTGATTGCGG CCAGCAGCAT TGCCCTGGCA 
GCCGAGGACC CTGTACTAAC TAACTCGGAG CGTAACAAAG TCCTGAGATA TTTCGACTAT 
GTTTTCACAG GAGTGTTTAC CTTTGAGATG GTTATAAAGA TGATAGACCA AGGCTTGATC 
CTGCAGGATG GATCATACTT CAGAGACCTC TGGAACATCC TGGACTTTGT AGTAGTGGTT 
GGTGCATTGG TAGCATTTGC TCTGGCGAAC GCTTTGGGAA CCAACAAGGG GAGGGATATC 
AAGACTATCA AGTCTCTGAG GGTACTTCGA GTTCTGCGGC CACTGAAAAC CATCAAGCGC 
TTGCCCAAGC TGAAGGCTGT GTTTGACTGC GTGGTGACCT CCTTGAAGAA TGTCTTCAAC 
ATACTCATTG TCTACAAGCT CTTCATGTTC ATCTTTGCCG TCATCGCTGT TCAGCTATTC 
AAAGGAAAGT TCTTTTATTG CACTGACAGT TCCAAGGACA CAGAGAAGGA GTGCATAGGC 
AACTATGTAG ACCACGAGAA AAACAAGATG GAGGTGAAGG GCCGGGAATG GAAGCGTCAT 
GAATTCCACT ATGACAACAT CATCTGGGCC CTGCTTACCC TCTTCACAGT CTCTACAGGG 
GAAGGATGGC CTCAGGTTCT GCAGCACTCT GTAGATGTGA CAGAGGAGGA CAGAGGCCCA 
AGTCGCAGCA ACCGCATGGA GATGTCCATC TTTTATGTGG TCTACTTTGT GGTCTTCCCT 
TTCTTCTTTG TCAATATCTT CGTGGCTCTC ATTATCATCA CCTTCCAGGA GCAAGGGGAC 
AAGATGATGG AGGAGTGTAG CCTGGAGAAG AATGAGCGGG CATGCATCGA CTTCGCCATT 
AGTGCCAAAC CTCTCACCCG CTACATGCCA CAGAACAGGC ACACCTTCCA GTACCGTGTG 
TGGCACTTTG TAGTCTCCCC ATCCTTTGAG TATACCATCA TGGCCATGAT TGCCTTGAAC 
ACTGTCGTGC TGATGATGAA GTACTACTCT GCTCCTTGCA CCTATGAGCT CGCCTTAAAG 
TACCTGAACA TCGCCTTCAC TATGGTGTTT TCCCTGGAGT GTGTCCTAAA GGTCATTGCT 
TTCGGCTTCC TGAACTATTT CCGAGATACC TGGAACATCT TTGACTTCAT CACGGTGATC 
GGCAGTATCA CAGAAATTAT CCTGACAGAT AGCAAGCTGG TGAACACCAG TGGCTTCAAT 
ATGAGCTTTC TGAAGCTCTT CCGAGCTGCA CGGCTCATAA AGCTTCTGAG ACAGGGCTAC 
ACCATCCGCA TCTTACTGTG GACCTTCGTG CAGTCCTTTA AGGCTCTTCC CTACGTCTGC 
CTTCTCATTG CCATGCTTTT CTTCATCTAC GCCATCATTG GGATGCAGGT GTTTGGAAAC 
ATAAAGTTAG ATGAGGAGAG TCACATCAAC CGGCACAACA ACTTCCGGAG TTTCTTTGGA 
TCCCTCATGC TGCTTTTCAG GAGTGCCACG GGGGAGGCTT GGCAGGAGAT TATGCTGTCC 
TGTCTCGGTG AGAAGGGTTG TGAGCCTGAC ACCACTGCAC CCTCAGGGCA GAATGAGAGT 
GAGCGCTGTG GCACTGACCT GGCCTACGTT TATTTTGTCT CCTTCATCTT CTTCTGCTCC 
TTCCTGATGC TCAACCTGTT CGTGGCTGTC ATCATGGACA ACTTTGAGTA CCTGACTCGA 
GATTCCTCCA TCCTGGGGCC TCACCACTTA GATGAGTTTG TCCGTGTCTG GGCAGAATAT 
GACAGAGCAG CATGTGGCCG CATCCATTAC ACTGAGATGT ATGAAATGCT GACTCTCATG 
TCACCACCGC TAGGCCTCGG CAAGAGATGT CCCTCCAAAG TGGCCTATAA GAGGCTGGTC 
CTGATGAACA TGCCAGTGGC TGAGGACATG ACAGTCCACT TCACCTCCAC ACTTATGGCT 
CTGATCCGGA CAGCTCTGGA CATTAAAATC GCCAAAGGTG GTGCAGACAG ACAGCAGCTA 
GACTCGGAGC TGCAAAAAGA GACCCTGGCC ATTTGGCCTC ACCTGTCCCA AAAGATGTTG 
GATCTGCTTG TGCCCATGCC CAAAGCCTCT GACCTGACTG TGGGCAAGAT CTATGCAGCA 
ATGATGATCA TGGACTACTA TAAACAGAGT AAGGTAAAGA AACAGAGACA GCAGCTGGAA 
GAACAGAAAA ATGCACCCAT GTTCCAGCGC ATGGAGCCCT CATCACTGCC TCAGGAGATC 
ATTGCTAATG CCAAAGCCCT GCCTTATCTC CAGCAGGACC CTGTTTCTGG CCTGAGTGGT 
CGGAGTGGAT ACCCTTCAAT GAGTCCACTC TCCCCTCAAG AAATATTCCA GTTGGCTTGT 
ATGGACCCAG CGGATGATGG ACAATTCCAG GAACAACAAT CTCTGGTGAA TGCACAAGAG 
CCAGAGGTTA GTGAATTAAA AAGTGTGCAG TCCTCTAACC ATGGCATCTA CCTTCCTCCG 
GACACCCAGG AGCATGCGGG ATCTGGGAGG GCATCCTCTA TGCCACGTCT GACTATGGAT 
CCCCAGGTGG TAACAGACCC TAGCTCTATG CGACGTTCAT TTTCTACAAT TCGGGATAAG 
CGTTCAAATT CCTCCTGGTT GGAGGAGTTC TCCATGGAAC GCAGCAGTGA AAACACTTAC 
AAGTCGCGGC GCAGAAGTTA CCATTCCTCC CTGAGGCTGT CAGCACACCG CCTGAATTCT 
GACTCTGGTC ACAAGTCTGA CACTCACCGA TCAGGAGGCA GGGAGAGAGG CCGATCCAAA 
GAACGAAAGC ACCTCCTCTC TCCAGATGTC TCCCGCTGCA ATTCTGAAGA GCGAGGGACC 
CAGGCCGACT GGGAGTCCCC AGAACGTCGC CAGTCCAGGT CACCCAGTGA AGGAAGGTCA 
CAGACCCCCA ATAGACAGGG TACCGGTTCC CTAAGTGAGA GCTCCATCCC CTCTATTTCC 
GACACCAGCA CCCCAAGACG AAGCCGTCGC CAGCTCCCAC CTGTGCCACC AAAGCCTCGT 
CCCCTCCTCT CCTACAGCTC CCTGATGAGA CACACTGGCG GCATCTCTCC ACCTCCTGAT 
GGAAGTGAGG GTGGATCCCC ACTGGCCTCT CAAGCTCTGG AGAGCAACAG CGCTTGCCTG 
ACCGAGTCTT CCAACTCCTT GCACCCCCAG CAGGGCCAGC ATCCTTCCCC ACAGCACTAC 
ATCTCCGAGC CCTATCTGGC CCTCCATGAA GACTCCCACG CCTCAGACTG TGGCGAGGAG 
GAGACACTCA CCTTTGAGGC AGCCGTAGCC ACTAGCTTGG GCCGGTCCAA TACCATTGGC 
TCTGCGCCAC CCCTACGGCA CAGCTGGCAG ATGCCTAATG GGCACTATCG GCGGCGGAGG 
CGTGGGGGGC CTGGGCCTGG CATGATGTGT GGAGCTGTCA GTGACCTCCT GAGTGACACG 
GAAGAAGACG ATAAGTGCTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030099789.1
CDS	93..7493
Translation	MVPEMRGLSPGRRLPEPTLCPATGGSPEPGAAGRRRHVLALPDEEAWRPQQCALLEANASDEVGMLPASPRSAVAGFDNFFPVQEGEGPGWEGSTTLDAGSSPFLPVSPEVMKRRRGGLIEQRDIIKAHEAHKMQSTPQARRKEWEMARFGEAVVVGRPGSGDGDSDQSRNRQGTPVPASGPAAAYKQSKAQRARTMALYNPIPVRQNCFTVNRSLFIFGEDNIVRKYAKKLIDWPPFEYMILATIIANCIVLALEQHLPEDDKTPMSRRLEKTEPYFIGIFCFEAGIKIVALGFIFHKGSYLRNGWNVMDFIVVLSGILATAGTHFNTHVDLRTLRAVRVLRPLKLVSGIPSLQIVLKSIMKAMVPLLQIGLLLFFAILMFAIIGLEFYSGKLHRACFMNNSGILEGFDPPHPCGVQGCPAGYECKDWIGPNDGITQFDNILFAVLTVFQCITMEGWTTVLYNTNDALGATWNWLYFIPLIIIGSFFVLNLVLGVLSGEFAKERERVENRRAFMKLRRQQQIERELNGYRAWIDKAEEVMLAEENKNSGTSALEVLRRATIKRSRTEAMTRDSSDEHCVDISSVGTPLARASIKSTKVDGASYFRHKERLLRISIRHMVKSQVFYWIVLSVVALNTACVAIVHHNQPQWLTHLLYYAEFLFLGLFLLEMSLKMYGMGPRLYFHSSFNCFDFGVTVGSIFEVVWAIFRPGTSFGISVLRALRLLRIFKITKYWASLRNLVVSLMSSMKSIISLLFLLFLFIVVFALLGMQLFGGRFNFNDGTPSANFDTFPAAIMTVFQILTGEDWNEVMYNGIRSQGGVSSGMWSAIYFIVLTLFGNYTLLNVFLAIAVDNLANAQELTKDEQEEEEAFNQKHALQKAKEVSPMSAPNMPSIERDRRRRHHMSMWEPRSSHLRERRRRHHMSVWEQRTSQLRRHMQMSSQEALNKEEAPPMNPLNPLNPLSPLNPLNAHPSLYRRPRPIEGLALGLGLEKCEEERISRGGSLKGDIGGLTSALDNQRSPLSLGKREPPWLPRSCHGNCDPIQQEAGGGETVVTFEDRARHRQSQRRSRHRRVRTEGKDSASASRSRSASQERSLDEGVSVEGEKEHEPHSSHRSKEPTIHEEERTQDLRRTNSLMVPRGSGLVGALDEAETPLVQPQPELEVGKDAALTEQEAEGSSEQALLGDVQLDVGRGISQSEPDLSCMTANMDKATTESTSVTVAIPDVDPLVDSTVVNISNKTDGEASPLKEAETKEEEEEVEKKKKQKKEKRETGKAMVPHSSMFIFSTTNPIRRACHYIVNLRYFEMCILLVIAASSIALAAEDPVLTNSERNKVLRYFDYVFTGVFTFEMVIKMIDQGLILQDGSYFRDLWNILDFVVVVGALVAFALANALGTNKGRDIKTIKSLRVLRVLRPLKTIKRLPKLKAVFDCVVTSLKNVFNILIVYKLFMFIFAVIAVQLFKGKFFYCTDSSKDTEKECIGNYVDHEKNKMEVKGREWKRHEFHYDNIIWALLTLFTVSTGEGWPQVLQHSVDVTEEDRGPSRSNRMEMSIFYVVYFVVFPFFFVNIFVALIIITFQEQGDKMMEECSLEKNERACIDFAISAKPLTRYMPQNRHTFQYRVWHFVVSPSFEYTIMAMIALNTVVLMMKYYSAPCTYELALKYLNIAFTMVFSLECVLKVIAFGFLNYFRDTWNIFDFITVIGSITEIILTDSKLVNTSGFNMSFLKLFRAARLIKLLRQGYTIRILLWTFVQSFKALPYVCLLIAMLFFIYAIIGMQVFGNIKLDEESHINRHNNFRSFFGSLMLLFRSATGEAWQEIMLSCLGEKGCEPDTTAPSGQNESERCGTDLAYVYFVSFIFFCSFLMLNLFVAVIMDNFEYLTRDSSILGPHHLDEFVRVWAEYDRAACGRIHYTEMYEMLTLMSPPLGLGKRCPSKVAYKRLVLMNMPVAEDMTVHFTSTLMALIRTALDIKIAKGGADRQQLDSELQKETLAIWPHLSQKMLDLLVPMPKASDLTVGKIYAAMMIMDYYKQSKVKKQRQQLEEQKNAPMFQRMEPSSLPQEIIANAKALPYLQQDPVSGLSGRSGYPSMSPLSPQEIFQLACMDPADDGQFQEQQSLVNAQEPEVSELKSVQSSNHGIYLPPDTQEHAGSGRASSMPRLTMDPQVVTDPSSMRRSFSTIRDKRSNSSWLEEFSMERSSENTYKSRRRSYHSSLRLSAHRLNSDSGHKSDTHRSGGRERGRSKERKHLLSPDVSRCNSEERGTQADWESPERRQSRSPSEGRSQTPNRQGTGSLSESSIPSISDTSTPRRSRRQLPPVPPKPRPLLSYSSLMRHTGGISPPPDGSEGGSPLASQALESNSACLTESSNSLHPQQGQHPSPQHYISEPYLALHEDSHASDCGEEETLTFEAAVATSLGRSNTIGSAPPLRHSWQMPNGHYRRRRRGGPGPGMMCGAVSDLLSDTEEDDKC

Target ORF information:

RefSeq Version	XM_030243929.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus calcium channel, voltage-dependent, R type, alpha 1E subunit (Cacna1e), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030243929.1

ORF Insert Sequence:

ATGGTGCCCG AGATGCGTGG ACTGAGCCCA GGCCGAAGGC TGCCCGAGCC CACCCTGTGC 
CCGGCTACAG GAGGGAGCCC TGAGCCCGGG GCTGCTGGCA GGCGCCGCCA CGTCCTGGCT 
CTGCCTGATG AGGAGGCATG GCGGCCGCAG CAGTGCGCCC TGCTCGAGGC CAATGCCTCC 
GACGAGGTGG GCATGTTGCC TGCTTCCCCG CGCTCCGCGG TGGCCGGCTT CGACAACTTC 
TTCCCGGTGC AGGAAGGCGA GGGCCCGGGC TGGGAGGGCA GCACCACGCT GGATGCAGGC 
TCCAGCCCCT TCCTTCCTGT GAGCCCAGAG GTCATGAAGC GGAGGCGAGG GGGGCTCATC 
GAGCAGCGGG ACATCATCAA GGCCCACGAG GCACACAAGA TGCAGAGCAC CCCGCAGGCC 
CGGCGCAAGG AGTGGGAGAT GGCTCGCTTC GGGGAGGCGG TGGTCGTTGG CAGGCCAGGC 
TCAGGCGATG GAGACTCGGA CCAGAGCAGG AACCGACAAG GAACCCCCGT CCCGGCCTCG 
GGGCCGGCGG CCGCCTACAA GCAGTCAAAA GCGCAGAGGG CGCGGACTAT GGCTTTGTAC 
AACCCCATAC CAGTCCGGCA GAACTGTTTC ACGGTCAACA GATCCCTGTT CATCTTCGGA 
GAAGATAACA TTGTCAGGAA ATACGCCAAG AAGCTCATCG ATTGGCCGCC ATTTGAGTAC 
ATGATCCTGG CCACCATCAT TGCCAACTGC ATCGTCTTGG CCCTGGAGCA GCATCTTCCT 
GAGGATGACA AGACCCCAAT GTCTCGAAGA CTGGAGAAGA CAGAACCATA TTTCATTGGG 
ATCTTCTGCT TTGAAGCTGG GATCAAAATT GTGGCTCTAG GGTTCATCTT CCATAAGGGT 
TCATACCTCC GTAATGGCTG GAATGTCATG GACTTCATCG TGGTCCTTAG TGGCATCCTG 
GCCACTGCGG GAACCCACTT CAACACCCAC GTGGACCTGC GGACCCTCCG GGCTGTGCGT 
GTCCTGAGGC CTTTAAAGCT GGTATCAGGA ATACCTAGCC TGCAGATCGT GCTGAAGTCT 
ATCATGAAGG CCATGGTGCC TCTTCTGCAG ATTGGCCTTC TGCTCTTCTT TGCCATCCTC 
ATGTTTGCTA TCATTGGCTT GGAGTTCTAC AGTGGCAAGT TGCATCGAGC ATGCTTCATG 
AACAATTCAG GTATTCTAGA GGGGTTTGAT CCTCCTCACC CGTGTGGTGT GCAAGGCTGC 
CCGGCTGGTT ATGAATGTAA GGACTGGATC GGCCCCAATG ACGGGATCAC CCAGTTTGAC 
AACATCCTTT TTGCCGTGCT GACTGTCTTC CAATGCATCA CTATGGAAGG GTGGACCACT 
GTGCTGTACA ATACCAATGA TGCCTTAGGA GCCACCTGGA ACTGGCTGTA CTTCATCCCT 
CTCATCATCA TCGGATCCTT CTTTGTTCTC AACCTCGTCC TGGGAGTGCT TTCCGGGGAA 
TTTGCCAAAG AAAGAGAGAG AGTGGAGAAC CGAAGAGCGT TCATGAAGCT CCGGCGCCAG 
CAACAGATTG AGCGAGAATT GAACGGCTAC CGTGCCTGGA TAGACAAAGC AGAGGAAGTC 
ATGCTTGCTG AAGAAAATAA AAACTCGGGA ACATCAGCCT TGGAAGTGCT TCGAAGGGCA 
ACCATCAAAA GGAGCCGGAC GGAGGCCATG ACCCGAGACT CCAGCGATGA GCACTGCGTT 
GATATCTCTT CAGTGGGCAC ACCTCTTGCC AGAGCCAGTA TCAAGAGCAC AAAAGTAGAT 
GGGGCCTCCT ATTTCCGGCA CAAGGAACGG CTTCTGCGCA TCTCTATCCG CCACATGGTC 
AAGTCCCAAG TCTTTTACTG GATCGTCCTG AGTGTTGTGG CTCTTAATAC TGCCTGTGTG 
GCCATTGTTC ATCACAACCA GCCACAGTGG CTCACTCACC TCCTCTACTA TGCAGAGTTT 
TTGTTTCTGG GACTCTTCCT GTTGGAGATG TCCCTGAAGA TGTATGGCAT GGGGCCACGC 
CTCTATTTTC ACTCATCCTT CAACTGCTTT GATTTTGGGG TCACAGTGGG CAGCATCTTT 
GAGGTTGTCT GGGCAATCTT CAGACCTGGC ACATCTTTTG GAATCAGTGT CTTACGAGCT 
CTCCGGCTCC TAAGGATATT TAAAATAACC AAGTATTGGG CATCCCTCCG GAATCTGGTT 
GTCTCCTTGA TGAGCTCCAT GAAGTCTATC ATCAGCTTAC TCTTCCTTCT CTTCCTCTTC 
ATCGTTGTCT TTGCTCTCCT GGGAATGCAG CTCTTTGGAG GCAGGTTTAA CTTTAATGAT 
GGGACTCCTT CAGCTAATTT TGACACCTTC CCTGCAGCTA TCATGACAGT GTTCCAGATC 
CTGACAGGGG AAGACTGGAA TGAGGTGATG TACAATGGCA TTCGCTCCCA GGGTGGGGTC 
AGCTCAGGCA TGTGGTCTGC CATCTACTTC ATTGTGCTCA CCTTGTTTGG AAACTACACG 
CTGCTAAATG TATTCTTGGC CATCGCTGTG GACAATCTGG CCAATGCCCA GGAACTGACC 
AAGGATGAGC AGGAGGAAGA AGAAGCCTTC AACCAGAAAC ATGCACTGCA GAAGGCCAAG 
GAGGTCAGCC CGATGTCTGC TCCCAACATG CCTTCCATTG AAAGAGACAG AAGGAGAAGA 
CACCACATGT CGATGTGGGA GCCACGCAGC AGCCACCTGA GGGAGCGGAG GCGCCGGCAC 
CACATGTCTG TGTGGGAACA ACGCACCAGC CAGCTGAGGA GGCACATGCA AATGTCTAGC 
CAAGAAGCCC TCAACAAAGA GGAGGCCCCG CCAATGAACC CCCTCAATCC ACTCAACCCA 
CTGAGTCCTC TCAACCCCCT CAATGCTCAC CCCAGCCTTT ATCGGAGGCC CAGGCCCATT 
GAGGGCCTAG CCCTAGGTCT GGGTCTTGAA AAGTGTGAAG AAGAACGCAT CAGTCGTGGG 
GGCTCCCTCA AGGGGGACAT AGGGGGCCTG ACTAGCGCCC TGGACAACCA GAGGAGCCCC 
TTGTCCTTGG GCAAACGGGA GCCACCGTGG CTGCCTAGAT CCTGTCATGG AAACTGTGAC 
CCAATCCAGC AGGAGGCTGG GGGAGGAGAG ACTGTGGTGA CTTTTGAGGA CCGGGCCAGG 
CACAGGCAGA GTCAGAGGCG AAGCCGGCAT CGCCGAGTCA GGACTGAGGG CAAGGACTCT 
GCCTCTGCAT CCCGAAGCAG GTCTGCCAGC CAGGAGCGGA GTCTGGATGA AGGGGTGTCC 
GTTGAGGGGG AGAAGGAACA TGAGCCTCAC AGCAGCCACA GGAGCAAAGA GCCGACCATC 
CACGAAGAGG AGAGAACCCA GGACTTAAGG AGGACCAACA GTCTGATGGT CCCCAGGGGC 
TCTGGACTGG TAGGGGCTCT TGATGAAGCA GAAACACCTC TAGTCCAGCC CCAACCTGAG 
CTGGAAGTGG GGAAGGATGC AGCTCTGACA GAGCAGGAGG CTGAAGGCAG CAGTGAACAA 
GCCCTTCTTG GGGACGTACA GCTGGATGTG GGCCGGGGCA TTAGCCAGAG TGAACCGGAT 
CTCTCCTGCA TGACAGCCAA CATGGACAAG GCTACCACGG AGAGCACCAG TGTCACGGTC 
GCCATCCCTG ATGTGGACCC CTTGGTGGAC TCGACAGTGG TGAACATTAG CAACAAGACG 
GATGGAGAAG CCAGTCCCTT GAAGGAGGCA GAGACTAAAG AGGAAGAAGA AGAGGTAGAG 
AAGAAGAAGA AGCAGAAGAA GGAGAAGCGA GAGACTGGAA AAGCCATGGT GCCCCATAGC 
TCTATGTTCA TCTTCAGCAC CACTAACCCG ATCCGCAGGG CCTGCCACTA CATTGTGAAC 
CTACGCTACT TTGAAATGTG CATTCTCTTG GTGATTGCGG CCAGCAGCAT TGCCCTGGCA 
GCCGAGGACC CTGTACTAAC TAACTCGGAG CGTAACAAAG TCCTGAGATA TTTCGACTAT 
GTTTTCACAG GAGTGTTTAC CTTTGAGATG GTTATAAAGA TGATAGACCA AGGCTTGATC 
CTGCAGGATG GATCATACTT CAGAGACCTC TGGAACATCC TGGACTTTGT AGTAGTGGTT 
GGTGCATTGG TAGCATTTGC TCTGGCGAAC GCTTTGGGAA CCAACAAGGG GAGGGATATC 
AAGACTATCA AGTCTCTGAG GGTACTTCGA GTTCTGCGGC CACTGAAAAC CATCAAGCGC 
TTGCCCAAGC TGAAGGCTGT GTTTGACTGC GTGGTGACCT CCTTGAAGAA TGTCTTCAAC 
ATACTCATTG TCTACAAGCT CTTCATGTTC ATCTTTGCCG TCATCGCTGT TCAGCTATTC 
AAAGGAAAGT TCTTTTATTG CACTGACAGT TCCAAGGACA CAGAGAAGGA GTGCATAGGC 
AACTATGTAG ACCACGAGAA AAACAAGATG GAGGTGAAGG GCCGGGAATG GAAGCGTCAT 
GAATTCCACT ATGACAACAT CATCTGGGCC CTGCTTACCC TCTTCACAGT CTCTACAGGG 
GAAGGATGGC CTCAGGTTCT GCAGCACTCT GTAGATGTGA CAGAGGAGGA CAGAGGCCCA 
AGTCGCAGCA ACCGCATGGA GATGTCCATC TTTTATGTGG TCTACTTTGT GGTCTTCCCT 
TTCTTCTTTG TCAATATCTT CGTGGCTCTC ATTATCATCA CCTTCCAGGA GCAAGGGGAC 
AAGATGATGG AGGAGTGTAG CCTGGAGAAG AATGAGCGGG CATGCATCGA CTTCGCCATT 
AGTGCCAAAC CTCTCACCCG CTACATGCCA CAGAACAGGC ACACCTTCCA GTACCGTGTG 
TGGCACTTTG TAGTCTCCCC ATCCTTTGAG TATACCATCA TGGCCATGAT TGCCTTGAAC 
ACTGTCGTGC TGATGATGAA GTACTACTCT GCTCCTTGCA CCTATGAGCT CGCCTTAAAG 
TACCTGAACA TCGCCTTCAC TATGGTGTTT TCCCTGGAGT GTGTCCTAAA GGTCATTGCT 
TTCGGCTTCC TGAACTATTT CCGAGATACC TGGAACATCT TTGACTTCAT CACGGTGATC 
GGCAGTATCA CAGAAATTAT CCTGACAGAT AGCAAGCTGG TGAACACCAG TGGCTTCAAT 
ATGAGCTTTC TGAAGCTCTT CCGAGCTGCA CGGCTCATAA AGCTTCTGAG ACAGGGCTAC 
ACCATCCGCA TCTTACTGTG GACCTTCGTG CAGTCCTTTA AGGCTCTTCC CTACGTCTGC 
CTTCTCATTG CCATGCTTTT CTTCATCTAC GCCATCATTG GGATGCAGGT GTTTGGAAAC 
ATAAAGTTAG ATGAGGAGAG TCACATCAAC CGGCACAACA ACTTCCGGAG TTTCTTTGGA 
TCCCTCATGC TGCTTTTCAG GAGTGCCACG GGGGAGGCTT GGCAGGAGAT TATGCTGTCC 
TGTCTCGGTG AGAAGGGTTG TGAGCCTGAC ACCACTGCAC CCTCAGGGCA GAATGAGAGT 
GAGCGCTGTG GCACTGACCT GGCCTACGTT TATTTTGTCT CCTTCATCTT CTTCTGCTCC 
TTCCTGATGC TCAACCTGTT CGTGGCTGTC ATCATGGACA ACTTTGAGTA CCTGACTCGA 
GATTCCTCCA TCCTGGGGCC TCACCACTTA GATGAGTTTG TCCGTGTCTG GGCAGAATAT 
GACAGAGCAG CATGTGGCCG CATCCATTAC ACTGAGATGT ATGAAATGCT GACTCTCATG 
TCACCACCGC TAGGCCTCGG CAAGAGATGT CCCTCCAAAG TGGCCTATAA GAGGCTGGTC 
CTGATGAACA TGCCAGTGGC TGAGGACATG ACAGTCCACT TCACCTCCAC ACTTATGGCT 
CTGATCCGGA CAGCTCTGGA CATTAAAATC GCCAAAGGTG GTGCAGACAG ACAGCAGCTA 
GACTCGGAGC TGCAAAAAGA GACCCTGGCC ATTTGGCCTC ACCTGTCCCA AAAGATGTTG 
GATCTGCTTG TGCCCATGCC CAAAGCCTCT GACCTGACTG TGGGCAAGAT CTATGCAGCA 
ATGATGATCA TGGACTACTA TAAACAGAGT AAGGTAAAGA AACAGAGACA GCAGCTGGAA 
GAACAGAAAA ATGCACCCAT GTTCCAGCGC ATGGAGCCCT CATCACTGCC TCAGGAGATC 
ATTGCTAATG CCAAAGCCCT GCCTTATCTC CAGCAGGACC CTGTTTCTGG CCTGAGTGGT 
CGGAGTGGAT ACCCTTCAAT GAGTCCACTC TCCCCTCAAG AAATATTCCA GTTGGCTTGT 
ATGGACCCAG CGGATGATGG ACAATTCCAG GAACAACAAT CTCTGGTGAA TGCACAAGAG 
CCAGAGGTTA GTGAATTAAA AAGTGTGCAG TCCTCTAACC ATGGCATCTA CCTTCCTCCG 
GACACCCAGG AGCATGCGGG ATCTGGGAGG GCATCCTCTA TGCCACGTCT GACTATGGAT 
CCCCAGGTGG TAACAGACCC TAGCTCTATG CGACGTTCAT TTTCTACAAT TCGGGATAAG 
CGTTCAAATT CCTCCTGGTT GGAGGAGTTC TCCATGGAAC GCAGCAGTGA AAACACTTAC 
AAGTCGCGGC GCAGAAGTTA CCATTCCTCC CTGAGGCTGT CAGCACACCG CCTGAATTCT 
GACTCTGGTC ACAAGTCTGA CACTCACCGA TCAGGAGGCA GGGAGAGAGG CCGATCCAAA 
GAACGAAAGC ACCTCCTCTC TCCAGATGTC TCCCGCTGCA ATTCTGAAGA GCGAGGGACC 
CAGGCCGACT GGGAGTCCCC AGAACGTCGC CAGTCCAGGT CACCCAGTGA AGGAAGGTCA 
CAGACCCCCA ATAGACAGGG TACCGGTTCC CTAAGTGAGA GCTCCATCCC CTCTATTTCC 
GACACCAGCA CCCCAAGACG AAGCCGTCGC CAGCTCCCAC CTGTGCCACC AAAGCCTCGT 
CCCCTCCTCT CCTACAGCTC CCTGATGAGA CACACTGGCG GCATCTCTCC ACCTCCTGAT 
GGAAGTGAGG GTGGATCCCC ACTGGCCTCT CAAGCTCTGG AGAGCAACAG CGCTTGCCTG 
ACCGAGTCTT CCAACTCCTT GCACCCCCAG CAGGGCCAGC ATCCTTCCCC ACAGCACTAC 
ATCTCCGAGC CCTATCTGGC CCTCCATGAA GACTCCCACG CCTCAGACTG TGGCGAGGAG 
GAGACACTCA CCTTTGAGGC AGCCGTAGCC ACTAGCTTGG GCCGGTCCAA TACCATTGGC 
TCTGCGCCAC CCCTACGGCA CAGCTGGCAG ATGCCTAATG GGCACTATCG GCGGCGGAGG 
CGTGGGGGGC CTGGGCCTGG CATGATGTGT GGAGCTGTCA GTGACCTCCT GAGTGACACG 
GAAGAAGACG ATAAGTGCTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cacna1e ( XM_017312578.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017168067.1 Mus musculus calcium channel, voltage-dependent, R type, alpha 1E subunit (Cacna1e), transcript variant X1, mRNA.

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CloneID	OMu67260
Clone ID Related Accession (Same CDS sequence)	XM_017312578.1 , XM_030243926.1 , XM_017312594.1 , XM_030243929.1 , XM_011247909.2 , XM_017312578.2 , XM_011247909.3
Accession Version	XM_017312578.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7401bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	voltage-dependent R-type calcium channel subunit alpha-1E isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_017312578.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGCCCG AGATGCGTGG ACTGAGCCCA GGCCGAAGGC TGCCCGAGCC CACCCTGTGC 
CCGGCTACAG GAGGGAGCCC TGAGCCCGGG GCTGCTGGCA GGCGCCGCCA CGTCCTGGCT 
CTGCCTGATG AGGAGGCATG GCGGCCGCAG CAGTGCGCCC TGCTCGAGGC CAATGCCTCC 
GACGAGGTGG GCATGTTGCC TGCTTCCCCG CGCTCCGCGG TGGCCGGCTT CGACAACTTC 
TTCCCGGTGC AGGAAGGCGA GGGCCCGGGC TGGGAGGGCA GCACCACGCT GGATGCAGGC 
TCCAGCCCCT TCCTTCCTGT GAGCCCAGAG GTCATGAAGC GGAGGCGAGG GGGGCTCATC 
GAGCAGCGGG ACATCATCAA GGCCCACGAG GCACACAAGA TGCAGAGCAC CCCGCAGGCC 
CGGCGCAAGG AGTGGGAGAT GGCTCGCTTC GGGGAGGCGG TGGTCGTTGG CAGGCCAGGC 
TCAGGCGATG GAGACTCGGA CCAGAGCAGG AACCGACAAG GAACCCCCGT CCCGGCCTCG 
GGGCCGGCGG CCGCCTACAA GCAGTCAAAA GCGCAGAGGG CGCGGACTAT GGCTTTGTAC 
AACCCCATAC CAGTCCGGCA GAACTGTTTC ACGGTCAACA GATCCCTGTT CATCTTCGGA 
GAAGATAACA TTGTCAGGAA ATACGCCAAG AAGCTCATCG ATTGGCCGCC ATTTGAGTAC 
ATGATCCTGG CCACCATCAT TGCCAACTGC ATCGTCTTGG CCCTGGAGCA GCATCTTCCT 
GAGGATGACA AGACCCCAAT GTCTCGAAGA CTGGAGAAGA CAGAACCATA TTTCATTGGG 
ATCTTCTGCT TTGAAGCTGG GATCAAAATT GTGGCTCTAG GGTTCATCTT CCATAAGGGT 
TCATACCTCC GTAATGGCTG GAATGTCATG GACTTCATCG TGGTCCTTAG TGGCATCCTG 
GCCACTGCGG GAACCCACTT CAACACCCAC GTGGACCTGC GGACCCTCCG GGCTGTGCGT 
GTCCTGAGGC CTTTAAAGCT GGTATCAGGA ATACCTAGCC TGCAGATCGT GCTGAAGTCT 
ATCATGAAGG CCATGGTGCC TCTTCTGCAG ATTGGCCTTC TGCTCTTCTT TGCCATCCTC 
ATGTTTGCTA TCATTGGCTT GGAGTTCTAC AGTGGCAAGT TGCATCGAGC ATGCTTCATG 
AACAATTCAG GTATTCTAGA GGGGTTTGAT CCTCCTCACC CGTGTGGTGT GCAAGGCTGC 
CCGGCTGGTT ATGAATGTAA GGACTGGATC GGCCCCAATG ACGGGATCAC CCAGTTTGAC 
AACATCCTTT TTGCCGTGCT GACTGTCTTC CAATGCATCA CTATGGAAGG GTGGACCACT 
GTGCTGTACA ATACCAATGA TGCCTTAGGA GCCACCTGGA ACTGGCTGTA CTTCATCCCT 
CTCATCATCA TCGGATCCTT CTTTGTTCTC AACCTCGTCC TGGGAGTGCT TTCCGGGGAA 
TTTGCCAAAG AAAGAGAGAG AGTGGAGAAC CGAAGAGCGT TCATGAAGCT CCGGCGCCAG 
CAACAGATTG AGCGAGAATT GAACGGCTAC CGTGCCTGGA TAGACAAAGC AGAGGAAGTC 
ATGCTTGCTG AAGAAAATAA AAACTCGGGA ACATCAGCCT TGGAAGTGCT TCGAAGGGCA 
ACCATCAAAA GGAGCCGGAC GGAGGCCATG ACCCGAGACT CCAGCGATGA GCACTGCGTT 
GATATCTCTT CAGTGGGCAC ACCTCTTGCC AGAGCCAGTA TCAAGAGCAC AAAAGTAGAT 
GGGGCCTCCT ATTTCCGGCA CAAGGAACGG CTTCTGCGCA TCTCTATCCG CCACATGGTC 
AAGTCCCAAG TCTTTTACTG GATCGTCCTG AGTGTTGTGG CTCTTAATAC TGCCTGTGTG 
GCCATTGTTC ATCACAACCA GCCACAGTGG CTCACTCACC TCCTCTACTA TGCAGAGTTT 
TTGTTTCTGG GACTCTTCCT GTTGGAGATG TCCCTGAAGA TGTATGGCAT GGGGCCACGC 
CTCTATTTTC ACTCATCCTT CAACTGCTTT GATTTTGGGG TCACAGTGGG CAGCATCTTT 
GAGGTTGTCT GGGCAATCTT CAGACCTGGC ACATCTTTTG GAATCAGTGT CTTACGAGCT 
CTCCGGCTCC TAAGGATATT TAAAATAACC AAGTATTGGG CATCCCTCCG GAATCTGGTT 
GTCTCCTTGA TGAGCTCCAT GAAGTCTATC ATCAGCTTAC TCTTCCTTCT CTTCCTCTTC 
ATCGTTGTCT TTGCTCTCCT GGGAATGCAG CTCTTTGGAG GCAGGTTTAA CTTTAATGAT 
GGGACTCCTT CAGCTAATTT TGACACCTTC CCTGCAGCTA TCATGACAGT GTTCCAGATC 
CTGACAGGGG AAGACTGGAA TGAGGTGATG TACAATGGCA TTCGCTCCCA GGGTGGGGTC 
AGCTCAGGCA TGTGGTCTGC CATCTACTTC ATTGTGCTCA CCTTGTTTGG AAACTACACG 
CTGCTAAATG TATTCTTGGC CATCGCTGTG GACAATCTGG CCAATGCCCA GGAACTGACC 
AAGGATGAGC AGGAGGAAGA AGAAGCCTTC AACCAGAAAC ATGCACTGCA GAAGGCCAAG 
GAGGTCAGCC CGATGTCTGC TCCCAACATG CCTTCCATTG AAAGAGACAG AAGGAGAAGA 
CACCACATGT CGATGTGGGA GCCACGCAGC AGCCACCTGA GGGAGCGGAG GCGCCGGCAC 
CACATGTCTG TGTGGGAACA ACGCACCAGC CAGCTGAGGA GGCACATGCA AATGTCTAGC 
CAAGAAGCCC TCAACAAAGA GGAGGCCCCG CCAATGAACC CCCTCAATCC ACTCAACCCA 
CTGAGTCCTC TCAACCCCCT CAATGCTCAC CCCAGCCTTT ATCGGAGGCC CAGGCCCATT 
GAGGGCCTAG CCCTAGGTCT GGGTCTTGAA AAGTGTGAAG AAGAACGCAT CAGTCGTGGG 
GGCTCCCTCA AGGGGGACAT AGGGGGCCTG ACTAGCGCCC TGGACAACCA GAGGAGCCCC 
TTGTCCTTGG GCAAACGGGA GCCACCGTGG CTGCCTAGAT CCTGTCATGG AAACTGTGAC 
CCAATCCAGC AGGAGGCTGG GGGAGGAGAG ACTGTGGTGA CTTTTGAGGA CCGGGCCAGG 
CACAGGCAGA GTCAGAGGCG AAGCCGGCAT CGCCGAGTCA GGACTGAGGG CAAGGACTCT 
GCCTCTGCAT CCCGAAGCAG GTCTGCCAGC CAGGAGCGGA GTCTGGATGA AGGGGTGTCC 
GTTGAGGGGG AGAAGGAACA TGAGCCTCAC AGCAGCCACA GGAGCAAAGA GCCGACCATC 
CACGAAGAGG AGAGAACCCA GGACTTAAGG AGGACCAACA GTCTGATGGT CCCCAGGGGC 
TCTGGACTGG TAGGGGCTCT TGATGAAGCA GAAACACCTC TAGTCCAGCC CCAACCTGAG 
CTGGAAGTGG GGAAGGATGC AGCTCTGACA GAGCAGGAGG CTGAAGGCAG CAGTGAACAA 
GCCCTTCTTG GGGACGTACA GCTGGATGTG GGCCGGGGCA TTAGCCAGAG TGAACCGGAT 
CTCTCCTGCA TGACAGCCAA CATGGACAAG GCTACCACGG AGAGCACCAG TGTCACGGTC 
GCCATCCCTG ATGTGGACCC CTTGGTGGAC TCGACAGTGG TGAACATTAG CAACAAGACG 
GATGGAGAAG CCAGTCCCTT GAAGGAGGCA GAGACTAAAG AGGAAGAAGA AGAGGTAGAG 
AAGAAGAAGA AGCAGAAGAA GGAGAAGCGA GAGACTGGAA AAGCCATGGT GCCCCATAGC 
TCTATGTTCA TCTTCAGCAC CACTAACCCG ATCCGCAGGG CCTGCCACTA CATTGTGAAC 
CTACGCTACT TTGAAATGTG CATTCTCTTG GTGATTGCGG CCAGCAGCAT TGCCCTGGCA 
GCCGAGGACC CTGTACTAAC TAACTCGGAG CGTAACAAAG TCCTGAGATA TTTCGACTAT 
GTTTTCACAG GAGTGTTTAC CTTTGAGATG GTTATAAAGA TGATAGACCA AGGCTTGATC 
CTGCAGGATG GATCATACTT CAGAGACCTC TGGAACATCC TGGACTTTGT AGTAGTGGTT 
GGTGCATTGG TAGCATTTGC TCTGGCGAAC GCTTTGGGAA CCAACAAGGG GAGGGATATC 
AAGACTATCA AGTCTCTGAG GGTACTTCGA GTTCTGCGGC CACTGAAAAC CATCAAGCGC 
TTGCCCAAGC TGAAGGCTGT GTTTGACTGC GTGGTGACCT CCTTGAAGAA TGTCTTCAAC 
ATACTCATTG TCTACAAGCT CTTCATGTTC ATCTTTGCCG TCATCGCTGT TCAGCTATTC 
AAAGGAAAGT TCTTTTATTG CACTGACAGT TCCAAGGACA CAGAGAAGGA GTGCATAGGC 
AACTATGTAG ACCACGAGAA AAACAAGATG GAGGTGAAGG GCCGGGAATG GAAGCGTCAT 
GAATTCCACT ATGACAACAT CATCTGGGCC CTGCTTACCC TCTTCACAGT CTCTACAGGG 
GAAGGATGGC CTCAGGTTCT GCAGCACTCT GTAGATGTGA CAGAGGAGGA CAGAGGCCCA 
AGTCGCAGCA ACCGCATGGA GATGTCCATC TTTTATGTGG TCTACTTTGT GGTCTTCCCT 
TTCTTCTTTG TCAATATCTT CGTGGCTCTC ATTATCATCA CCTTCCAGGA GCAAGGGGAC 
AAGATGATGG AGGAGTGTAG CCTGGAGAAG AATGAGCGGG CATGCATCGA CTTCGCCATT 
AGTGCCAAAC CTCTCACCCG CTACATGCCA CAGAACAGGC ACACCTTCCA GTACCGTGTG 
TGGCACTTTG TAGTCTCCCC ATCCTTTGAG TATACCATCA TGGCCATGAT TGCCTTGAAC 
ACTGTCGTGC TGATGATGAA GTACTACTCT GCTCCTTGCA CCTATGAGCT CGCCTTAAAG 
TACCTGAACA TCGCCTTCAC TATGGTGTTT TCCCTGGAGT GTGTCCTAAA GGTCATTGCT 
TTCGGCTTCC TGAACTATTT CCGAGATACC TGGAACATCT TTGACTTCAT CACGGTGATC 
GGCAGTATCA CAGAAATTAT CCTGACAGAT AGCAAGCTGG TGAACACCAG TGGCTTCAAT 
ATGAGCTTTC TGAAGCTCTT CCGAGCTGCA CGGCTCATAA AGCTTCTGAG ACAGGGCTAC 
ACCATCCGCA TCTTACTGTG GACCTTCGTG CAGTCCTTTA AGGCTCTTCC CTACGTCTGC 
CTTCTCATTG CCATGCTTTT CTTCATCTAC GCCATCATTG GGATGCAGGT GTTTGGAAAC 
ATAAAGTTAG ATGAGGAGAG TCACATCAAC CGGCACAACA ACTTCCGGAG TTTCTTTGGA 
TCCCTCATGC TGCTTTTCAG GAGTGCCACG GGGGAGGCTT GGCAGGAGAT TATGCTGTCC 
TGTCTCGGTG AGAAGGGTTG TGAGCCTGAC ACCACTGCAC CCTCAGGGCA GAATGAGAGT 
GAGCGCTGTG GCACTGACCT GGCCTACGTT TATTTTGTCT CCTTCATCTT CTTCTGCTCC 
TTCCTGATGC TCAACCTGTT CGTGGCTGTC ATCATGGACA ACTTTGAGTA CCTGACTCGA 
GATTCCTCCA TCCTGGGGCC TCACCACTTA GATGAGTTTG TCCGTGTCTG GGCAGAATAT 
GACAGAGCAG CATGTGGCCG CATCCATTAC ACTGAGATGT ATGAAATGCT GACTCTCATG 
TCACCACCGC TAGGCCTCGG CAAGAGATGT CCCTCCAAAG TGGCCTATAA GAGGCTGGTC 
CTGATGAACA TGCCAGTGGC TGAGGACATG ACAGTCCACT TCACCTCCAC ACTTATGGCT 
CTGATCCGGA CAGCTCTGGA CATTAAAATC GCCAAAGGTG GTGCAGACAG ACAGCAGCTA 
GACTCGGAGC TGCAAAAAGA GACCCTGGCC ATTTGGCCTC ACCTGTCCCA AAAGATGTTG 
GATCTGCTTG TGCCCATGCC CAAAGCCTCT GACCTGACTG TGGGCAAGAT CTATGCAGCA 
ATGATGATCA TGGACTACTA TAAACAGAGT AAGGTAAAGA AACAGAGACA GCAGCTGGAA 
GAACAGAAAA ATGCACCCAT GTTCCAGCGC ATGGAGCCCT CATCACTGCC TCAGGAGATC 
ATTGCTAATG CCAAAGCCCT GCCTTATCTC CAGCAGGACC CTGTTTCTGG CCTGAGTGGT 
CGGAGTGGAT ACCCTTCAAT GAGTCCACTC TCCCCTCAAG AAATATTCCA GTTGGCTTGT 
ATGGACCCAG CGGATGATGG ACAATTCCAG GAACAACAAT CTCTGGTGAA TGCACAAGAG 
CCAGAGGTTA GTGAATTAAA AAGTGTGCAG TCCTCTAACC ATGGCATCTA CCTTCCTCCG 
GACACCCAGG AGCATGCGGG ATCTGGGAGG GCATCCTCTA TGCCACGTCT GACTATGGAT 
CCCCAGGTGG TAACAGACCC TAGCTCTATG CGACGTTCAT TTTCTACAAT TCGGGATAAG 
CGTTCAAATT CCTCCTGGTT GGAGGAGTTC TCCATGGAAC GCAGCAGTGA AAACACTTAC 
AAGTCGCGGC GCAGAAGTTA CCATTCCTCC CTGAGGCTGT CAGCACACCG CCTGAATTCT 
GACTCTGGTC ACAAGTCTGA CACTCACCGA TCAGGAGGCA GGGAGAGAGG CCGATCCAAA 
GAACGAAAGC ACCTCCTCTC TCCAGATGTC TCCCGCTGCA ATTCTGAAGA GCGAGGGACC 
CAGGCCGACT GGGAGTCCCC AGAACGTCGC CAGTCCAGGT CACCCAGTGA AGGAAGGTCA 
CAGACCCCCA ATAGACAGGG TACCGGTTCC CTAAGTGAGA GCTCCATCCC CTCTATTTCC 
GACACCAGCA CCCCAAGACG AAGCCGTCGC CAGCTCCCAC CTGTGCCACC AAAGCCTCGT 
CCCCTCCTCT CCTACAGCTC CCTGATGAGA CACACTGGCG GCATCTCTCC ACCTCCTGAT 
GGAAGTGAGG GTGGATCCCC ACTGGCCTCT CAAGCTCTGG AGAGCAACAG CGCTTGCCTG 
ACCGAGTCTT CCAACTCCTT GCACCCCCAG CAGGGCCAGC ATCCTTCCCC ACAGCACTAC 
ATCTCCGAGC CCTATCTGGC CCTCCATGAA GACTCCCACG CCTCAGACTG TGGCGAGGAG 
GAGACACTCA CCTTTGAGGC AGCCGTAGCC ACTAGCTTGG GCCGGTCCAA TACCATTGGC 
TCTGCGCCAC CCCTACGGCA CAGCTGGCAG ATGCCTAATG GGCACTATCG GCGGCGGAGG 
CGTGGGGGGC CTGGGCCTGG CATGATGTGT GGAGCTGTCA GTGACCTCCT GAGTGACACG 
GAAGAAGACG ATAAGTGCTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017168067.1
CDS	598..7998
Translation	MVPEMRGLSPGRRLPEPTLCPATGGSPEPGAAGRRRHVLALPDEEAWRPQQCALLEANASDEVGMLPASPRSAVAGFDNFFPVQEGEGPGWEGSTTLDAGSSPFLPVSPEVMKRRRGGLIEQRDIIKAHEAHKMQSTPQARRKEWEMARFGEAVVVGRPGSGDGDSDQSRNRQGTPVPASGPAAAYKQSKAQRARTMALYNPIPVRQNCFTVNRSLFIFGEDNIVRKYAKKLIDWPPFEYMILATIIANCIVLALEQHLPEDDKTPMSRRLEKTEPYFIGIFCFEAGIKIVALGFIFHKGSYLRNGWNVMDFIVVLSGILATAGTHFNTHVDLRTLRAVRVLRPLKLVSGIPSLQIVLKSIMKAMVPLLQIGLLLFFAILMFAIIGLEFYSGKLHRACFMNNSGILEGFDPPHPCGVQGCPAGYECKDWIGPNDGITQFDNILFAVLTVFQCITMEGWTTVLYNTNDALGATWNWLYFIPLIIIGSFFVLNLVLGVLSGEFAKERERVENRRAFMKLRRQQQIERELNGYRAWIDKAEEVMLAEENKNSGTSALEVLRRATIKRSRTEAMTRDSSDEHCVDISSVGTPLARASIKSTKVDGASYFRHKERLLRISIRHMVKSQVFYWIVLSVVALNTACVAIVHHNQPQWLTHLLYYAEFLFLGLFLLEMSLKMYGMGPRLYFHSSFNCFDFGVTVGSIFEVVWAIFRPGTSFGISVLRALRLLRIFKITKYWASLRNLVVSLMSSMKSIISLLFLLFLFIVVFALLGMQLFGGRFNFNDGTPSANFDTFPAAIMTVFQILTGEDWNEVMYNGIRSQGGVSSGMWSAIYFIVLTLFGNYTLLNVFLAIAVDNLANAQELTKDEQEEEEAFNQKHALQKAKEVSPMSAPNMPSIERDRRRRHHMSMWEPRSSHLRERRRRHHMSVWEQRTSQLRRHMQMSSQEALNKEEAPPMNPLNPLNPLSPLNPLNAHPSLYRRPRPIEGLALGLGLEKCEEERISRGGSLKGDIGGLTSALDNQRSPLSLGKREPPWLPRSCHGNCDPIQQEAGGGETVVTFEDRARHRQSQRRSRHRRVRTEGKDSASASRSRSASQERSLDEGVSVEGEKEHEPHSSHRSKEPTIHEEERTQDLRRTNSLMVPRGSGLVGALDEAETPLVQPQPELEVGKDAALTEQEAEGSSEQALLGDVQLDVGRGISQSEPDLSCMTANMDKATTESTSVTVAIPDVDPLVDSTVVNISNKTDGEASPLKEAETKEEEEEVEKKKKQKKEKRETGKAMVPHSSMFIFSTTNPIRRACHYIVNLRYFEMCILLVIAASSIALAAEDPVLTNSERNKVLRYFDYVFTGVFTFEMVIKMIDQGLILQDGSYFRDLWNILDFVVVVGALVAFALANALGTNKGRDIKTIKSLRVLRVLRPLKTIKRLPKLKAVFDCVVTSLKNVFNILIVYKLFMFIFAVIAVQLFKGKFFYCTDSSKDTEKECIGNYVDHEKNKMEVKGREWKRHEFHYDNIIWALLTLFTVSTGEGWPQVLQHSVDVTEEDRGPSRSNRMEMSIFYVVYFVVFPFFFVNIFVALIIITFQEQGDKMMEECSLEKNERACIDFAISAKPLTRYMPQNRHTFQYRVWHFVVSPSFEYTIMAMIALNTVVLMMKYYSAPCTYELALKYLNIAFTMVFSLECVLKVIAFGFLNYFRDTWNIFDFITVIGSITEIILTDSKLVNTSGFNMSFLKLFRAARLIKLLRQGYTIRILLWTFVQSFKALPYVCLLIAMLFFIYAIIGMQVFGNIKLDEESHINRHNNFRSFFGSLMLLFRSATGEAWQEIMLSCLGEKGCEPDTTAPSGQNESERCGTDLAYVYFVSFIFFCSFLMLNLFVAVIMDNFEYLTRDSSILGPHHLDEFVRVWAEYDRAACGRIHYTEMYEMLTLMSPPLGLGKRCPSKVAYKRLVLMNMPVAEDMTVHFTSTLMALIRTALDIKIAKGGADRQQLDSELQKETLAIWPHLSQKMLDLLVPMPKASDLTVGKIYAAMMIMDYYKQSKVKKQRQQLEEQKNAPMFQRMEPSSLPQEIIANAKALPYLQQDPVSGLSGRSGYPSMSPLSPQEIFQLACMDPADDGQFQEQQSLVNAQEPEVSELKSVQSSNHGIYLPPDTQEHAGSGRASSMPRLTMDPQVVTDPSSMRRSFSTIRDKRSNSSWLEEFSMERSSENTYKSRRRSYHSSLRLSAHRLNSDSGHKSDTHRSGGRERGRSKERKHLLSPDVSRCNSEERGTQADWESPERRQSRSPSEGRSQTPNRQGTGSLSESSIPSISDTSTPRRSRRQLPPVPPKPRPLLSYSSLMRHTGGISPPPDGSEGGSPLASQALESNSACLTESSNSLHPQQGQHPSPQHYISEPYLALHEDSHASDCGEEETLTFEAAVATSLGRSNTIGSAPPLRHSWQMPNGHYRRRRRGGPGPGMMCGAVSDLLSDTEEDDKC

Target ORF information:

RefSeq Version	XM_017312578.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus calcium channel, voltage-dependent, R type, alpha 1E subunit (Cacna1e), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017312578.2

ORF Insert Sequence:

ATGGTGCCCG AGATGCGTGG ACTGAGCCCA GGCCGAAGGC TGCCCGAGCC CACCCTGTGC 
CCGGCTACAG GAGGGAGCCC TGAGCCCGGG GCTGCTGGCA GGCGCCGCCA CGTCCTGGCT 
CTGCCTGATG AGGAGGCATG GCGGCCGCAG CAGTGCGCCC TGCTCGAGGC CAATGCCTCC 
GACGAGGTGG GCATGTTGCC TGCTTCCCCG CGCTCCGCGG TGGCCGGCTT CGACAACTTC 
TTCCCGGTGC AGGAAGGCGA GGGCCCGGGC TGGGAGGGCA GCACCACGCT GGATGCAGGC 
TCCAGCCCCT TCCTTCCTGT GAGCCCAGAG GTCATGAAGC GGAGGCGAGG GGGGCTCATC 
GAGCAGCGGG ACATCATCAA GGCCCACGAG GCACACAAGA TGCAGAGCAC CCCGCAGGCC 
CGGCGCAAGG AGTGGGAGAT GGCTCGCTTC GGGGAGGCGG TGGTCGTTGG CAGGCCAGGC 
TCAGGCGATG GAGACTCGGA CCAGAGCAGG AACCGACAAG GAACCCCCGT CCCGGCCTCG 
GGGCCGGCGG CCGCCTACAA GCAGTCAAAA GCGCAGAGGG CGCGGACTAT GGCTTTGTAC 
AACCCCATAC CAGTCCGGCA GAACTGTTTC ACGGTCAACA GATCCCTGTT CATCTTCGGA 
GAAGATAACA TTGTCAGGAA ATACGCCAAG AAGCTCATCG ATTGGCCGCC ATTTGAGTAC 
ATGATCCTGG CCACCATCAT TGCCAACTGC ATCGTCTTGG CCCTGGAGCA GCATCTTCCT 
GAGGATGACA AGACCCCAAT GTCTCGAAGA CTGGAGAAGA CAGAACCATA TTTCATTGGG 
ATCTTCTGCT TTGAAGCTGG GATCAAAATT GTGGCTCTAG GGTTCATCTT CCATAAGGGT 
TCATACCTCC GTAATGGCTG GAATGTCATG GACTTCATCG TGGTCCTTAG TGGCATCCTG 
GCCACTGCGG GAACCCACTT CAACACCCAC GTGGACCTGC GGACCCTCCG GGCTGTGCGT 
GTCCTGAGGC CTTTAAAGCT GGTATCAGGA ATACCTAGCC TGCAGATCGT GCTGAAGTCT 
ATCATGAAGG CCATGGTGCC TCTTCTGCAG ATTGGCCTTC TGCTCTTCTT TGCCATCCTC 
ATGTTTGCTA TCATTGGCTT GGAGTTCTAC AGTGGCAAGT TGCATCGAGC ATGCTTCATG 
AACAATTCAG GTATTCTAGA GGGGTTTGAT CCTCCTCACC CGTGTGGTGT GCAAGGCTGC 
CCGGCTGGTT ATGAATGTAA GGACTGGATC GGCCCCAATG ACGGGATCAC CCAGTTTGAC 
AACATCCTTT TTGCCGTGCT GACTGTCTTC CAATGCATCA CTATGGAAGG GTGGACCACT 
GTGCTGTACA ATACCAATGA TGCCTTAGGA GCCACCTGGA ACTGGCTGTA CTTCATCCCT 
CTCATCATCA TCGGATCCTT CTTTGTTCTC AACCTCGTCC TGGGAGTGCT TTCCGGGGAA 
TTTGCCAAAG AAAGAGAGAG AGTGGAGAAC CGAAGAGCGT TCATGAAGCT CCGGCGCCAG 
CAACAGATTG AGCGAGAATT GAACGGCTAC CGTGCCTGGA TAGACAAAGC AGAGGAAGTC 
ATGCTTGCTG AAGAAAATAA AAACTCGGGA ACATCAGCCT TGGAAGTGCT TCGAAGGGCA 
ACCATCAAAA GGAGCCGGAC GGAGGCCATG ACCCGAGACT CCAGCGATGA GCACTGCGTT 
GATATCTCTT CAGTGGGCAC ACCTCTTGCC AGAGCCAGTA TCAAGAGCAC AAAAGTAGAT 
GGGGCCTCCT ATTTCCGGCA CAAGGAACGG CTTCTGCGCA TCTCTATCCG CCACATGGTC 
AAGTCCCAAG TCTTTTACTG GATCGTCCTG AGTGTTGTGG CTCTTAATAC TGCCTGTGTG 
GCCATTGTTC ATCACAACCA GCCACAGTGG CTCACTCACC TCCTCTACTA TGCAGAGTTT 
TTGTTTCTGG GACTCTTCCT GTTGGAGATG TCCCTGAAGA TGTATGGCAT GGGGCCACGC 
CTCTATTTTC ACTCATCCTT CAACTGCTTT GATTTTGGGG TCACAGTGGG CAGCATCTTT 
GAGGTTGTCT GGGCAATCTT CAGACCTGGC ACATCTTTTG GAATCAGTGT CTTACGAGCT 
CTCCGGCTCC TAAGGATATT TAAAATAACC AAGTATTGGG CATCCCTCCG GAATCTGGTT 
GTCTCCTTGA TGAGCTCCAT GAAGTCTATC ATCAGCTTAC TCTTCCTTCT CTTCCTCTTC 
ATCGTTGTCT TTGCTCTCCT GGGAATGCAG CTCTTTGGAG GCAGGTTTAA CTTTAATGAT 
GGGACTCCTT CAGCTAATTT TGACACCTTC CCTGCAGCTA TCATGACAGT GTTCCAGATC 
CTGACAGGGG AAGACTGGAA TGAGGTGATG TACAATGGCA TTCGCTCCCA GGGTGGGGTC 
AGCTCAGGCA TGTGGTCTGC CATCTACTTC ATTGTGCTCA CCTTGTTTGG AAACTACACG 
CTGCTAAATG TATTCTTGGC CATCGCTGTG GACAATCTGG CCAATGCCCA GGAACTGACC 
AAGGATGAGC AGGAGGAAGA AGAAGCCTTC AACCAGAAAC ATGCACTGCA GAAGGCCAAG 
GAGGTCAGCC CGATGTCTGC TCCCAACATG CCTTCCATTG AAAGAGACAG AAGGAGAAGA 
CACCACATGT CGATGTGGGA GCCACGCAGC AGCCACCTGA GGGAGCGGAG GCGCCGGCAC 
CACATGTCTG TGTGGGAACA ACGCACCAGC CAGCTGAGGA GGCACATGCA AATGTCTAGC 
CAAGAAGCCC TCAACAAAGA GGAGGCCCCG CCAATGAACC CCCTCAATCC ACTCAACCCA 
CTGAGTCCTC TCAACCCCCT CAATGCTCAC CCCAGCCTTT ATCGGAGGCC CAGGCCCATT 
GAGGGCCTAG CCCTAGGTCT GGGTCTTGAA AAGTGTGAAG AAGAACGCAT CAGTCGTGGG 
GGCTCCCTCA AGGGGGACAT AGGGGGCCTG ACTAGCGCCC TGGACAACCA GAGGAGCCCC 
TTGTCCTTGG GCAAACGGGA GCCACCGTGG CTGCCTAGAT CCTGTCATGG AAACTGTGAC 
CCAATCCAGC AGGAGGCTGG GGGAGGAGAG ACTGTGGTGA CTTTTGAGGA CCGGGCCAGG 
CACAGGCAGA GTCAGAGGCG AAGCCGGCAT CGCCGAGTCA GGACTGAGGG CAAGGACTCT 
GCCTCTGCAT CCCGAAGCAG GTCTGCCAGC CAGGAGCGGA GTCTGGATGA AGGGGTGTCC 
GTTGAGGGGG AGAAGGAACA TGAGCCTCAC AGCAGCCACA GGAGCAAAGA GCCGACCATC 
CACGAAGAGG AGAGAACCCA GGACTTAAGG AGGACCAACA GTCTGATGGT CCCCAGGGGC 
TCTGGACTGG TAGGGGCTCT TGATGAAGCA GAAACACCTC TAGTCCAGCC CCAACCTGAG 
CTGGAAGTGG GGAAGGATGC AGCTCTGACA GAGCAGGAGG CTGAAGGCAG CAGTGAACAA 
GCCCTTCTTG GGGACGTACA GCTGGATGTG GGCCGGGGCA TTAGCCAGAG TGAACCGGAT 
CTCTCCTGCA TGACAGCCAA CATGGACAAG GCTACCACGG AGAGCACCAG TGTCACGGTC 
GCCATCCCTG ATGTGGACCC CTTGGTGGAC TCGACAGTGG TGAACATTAG CAACAAGACG 
GATGGAGAAG CCAGTCCCTT GAAGGAGGCA GAGACTAAAG AGGAAGAAGA AGAGGTAGAG 
AAGAAGAAGA AGCAGAAGAA GGAGAAGCGA GAGACTGGAA AAGCCATGGT GCCCCATAGC 
TCTATGTTCA TCTTCAGCAC CACTAACCCG ATCCGCAGGG CCTGCCACTA CATTGTGAAC 
CTACGCTACT TTGAAATGTG CATTCTCTTG GTGATTGCGG CCAGCAGCAT TGCCCTGGCA 
GCCGAGGACC CTGTACTAAC TAACTCGGAG CGTAACAAAG TCCTGAGATA TTTCGACTAT 
GTTTTCACAG GAGTGTTTAC CTTTGAGATG GTTATAAAGA TGATAGACCA AGGCTTGATC 
CTGCAGGATG GATCATACTT CAGAGACCTC TGGAACATCC TGGACTTTGT AGTAGTGGTT 
GGTGCATTGG TAGCATTTGC TCTGGCGAAC GCTTTGGGAA CCAACAAGGG GAGGGATATC 
AAGACTATCA AGTCTCTGAG GGTACTTCGA GTTCTGCGGC CACTGAAAAC CATCAAGCGC 
TTGCCCAAGC TGAAGGCTGT GTTTGACTGC GTGGTGACCT CCTTGAAGAA TGTCTTCAAC 
ATACTCATTG TCTACAAGCT CTTCATGTTC ATCTTTGCCG TCATCGCTGT TCAGCTATTC 
AAAGGAAAGT TCTTTTATTG CACTGACAGT TCCAAGGACA CAGAGAAGGA GTGCATAGGC 
AACTATGTAG ACCACGAGAA AAACAAGATG GAGGTGAAGG GCCGGGAATG GAAGCGTCAT 
GAATTCCACT ATGACAACAT CATCTGGGCC CTGCTTACCC TCTTCACAGT CTCTACAGGG 
GAAGGATGGC CTCAGGTTCT GCAGCACTCT GTAGATGTGA CAGAGGAGGA CAGAGGCCCA 
AGTCGCAGCA ACCGCATGGA GATGTCCATC TTTTATGTGG TCTACTTTGT GGTCTTCCCT 
TTCTTCTTTG TCAATATCTT CGTGGCTCTC ATTATCATCA CCTTCCAGGA GCAAGGGGAC 
AAGATGATGG AGGAGTGTAG CCTGGAGAAG AATGAGCGGG CATGCATCGA CTTCGCCATT 
AGTGCCAAAC CTCTCACCCG CTACATGCCA CAGAACAGGC ACACCTTCCA GTACCGTGTG 
TGGCACTTTG TAGTCTCCCC ATCCTTTGAG TATACCATCA TGGCCATGAT TGCCTTGAAC 
ACTGTCGTGC TGATGATGAA GTACTACTCT GCTCCTTGCA CCTATGAGCT CGCCTTAAAG 
TACCTGAACA TCGCCTTCAC TATGGTGTTT TCCCTGGAGT GTGTCCTAAA GGTCATTGCT 
TTCGGCTTCC TGAACTATTT CCGAGATACC TGGAACATCT TTGACTTCAT CACGGTGATC 
GGCAGTATCA CAGAAATTAT CCTGACAGAT AGCAAGCTGG TGAACACCAG TGGCTTCAAT 
ATGAGCTTTC TGAAGCTCTT CCGAGCTGCA CGGCTCATAA AGCTTCTGAG ACAGGGCTAC 
ACCATCCGCA TCTTACTGTG GACCTTCGTG CAGTCCTTTA AGGCTCTTCC CTACGTCTGC 
CTTCTCATTG CCATGCTTTT CTTCATCTAC GCCATCATTG GGATGCAGGT GTTTGGAAAC 
ATAAAGTTAG ATGAGGAGAG TCACATCAAC CGGCACAACA ACTTCCGGAG TTTCTTTGGA 
TCCCTCATGC TGCTTTTCAG GAGTGCCACG GGGGAGGCTT GGCAGGAGAT TATGCTGTCC 
TGTCTCGGTG AGAAGGGTTG TGAGCCTGAC ACCACTGCAC CCTCAGGGCA GAATGAGAGT 
GAGCGCTGTG GCACTGACCT GGCCTACGTT TATTTTGTCT CCTTCATCTT CTTCTGCTCC 
TTCCTGATGC TCAACCTGTT CGTGGCTGTC ATCATGGACA ACTTTGAGTA CCTGACTCGA 
GATTCCTCCA TCCTGGGGCC TCACCACTTA GATGAGTTTG TCCGTGTCTG GGCAGAATAT 
GACAGAGCAG CATGTGGCCG CATCCATTAC ACTGAGATGT ATGAAATGCT GACTCTCATG 
TCACCACCGC TAGGCCTCGG CAAGAGATGT CCCTCCAAAG TGGCCTATAA GAGGCTGGTC 
CTGATGAACA TGCCAGTGGC TGAGGACATG ACAGTCCACT TCACCTCCAC ACTTATGGCT 
CTGATCCGGA CAGCTCTGGA CATTAAAATC GCCAAAGGTG GTGCAGACAG ACAGCAGCTA 
GACTCGGAGC TGCAAAAAGA GACCCTGGCC ATTTGGCCTC ACCTGTCCCA AAAGATGTTG 
GATCTGCTTG TGCCCATGCC CAAAGCCTCT GACCTGACTG TGGGCAAGAT CTATGCAGCA 
ATGATGATCA TGGACTACTA TAAACAGAGT AAGGTAAAGA AACAGAGACA GCAGCTGGAA 
GAACAGAAAA ATGCACCCAT GTTCCAGCGC ATGGAGCCCT CATCACTGCC TCAGGAGATC 
ATTGCTAATG CCAAAGCCCT GCCTTATCTC CAGCAGGACC CTGTTTCTGG CCTGAGTGGT 
CGGAGTGGAT ACCCTTCAAT GAGTCCACTC TCCCCTCAAG AAATATTCCA GTTGGCTTGT 
ATGGACCCAG CGGATGATGG ACAATTCCAG GAACAACAAT CTCTGGTGAA TGCACAAGAG 
CCAGAGGTTA GTGAATTAAA AAGTGTGCAG TCCTCTAACC ATGGCATCTA CCTTCCTCCG 
GACACCCAGG AGCATGCGGG ATCTGGGAGG GCATCCTCTA TGCCACGTCT GACTATGGAT 
CCCCAGGTGG TAACAGACCC TAGCTCTATG CGACGTTCAT TTTCTACAAT TCGGGATAAG 
CGTTCAAATT CCTCCTGGTT GGAGGAGTTC TCCATGGAAC GCAGCAGTGA AAACACTTAC 
AAGTCGCGGC GCAGAAGTTA CCATTCCTCC CTGAGGCTGT CAGCACACCG CCTGAATTCT 
GACTCTGGTC ACAAGTCTGA CACTCACCGA TCAGGAGGCA GGGAGAGAGG CCGATCCAAA 
GAACGAAAGC ACCTCCTCTC TCCAGATGTC TCCCGCTGCA ATTCTGAAGA GCGAGGGACC 
CAGGCCGACT GGGAGTCCCC AGAACGTCGC CAGTCCAGGT CACCCAGTGA AGGAAGGTCA 
CAGACCCCCA ATAGACAGGG TACCGGTTCC CTAAGTGAGA GCTCCATCCC CTCTATTTCC 
GACACCAGCA CCCCAAGACG AAGCCGTCGC CAGCTCCCAC CTGTGCCACC AAAGCCTCGT 
CCCCTCCTCT CCTACAGCTC CCTGATGAGA CACACTGGCG GCATCTCTCC ACCTCCTGAT 
GGAAGTGAGG GTGGATCCCC ACTGGCCTCT CAAGCTCTGG AGAGCAACAG CGCTTGCCTG 
ACCGAGTCTT CCAACTCCTT GCACCCCCAG CAGGGCCAGC ATCCTTCCCC ACAGCACTAC 
ATCTCCGAGC CCTATCTGGC CCTCCATGAA GACTCCCACG CCTCAGACTG TGGCGAGGAG 
GAGACACTCA CCTTTGAGGC AGCCGTAGCC ACTAGCTTGG GCCGGTCCAA TACCATTGGC 
TCTGCGCCAC CCCTACGGCA CAGCTGGCAG ATGCCTAATG GGCACTATCG GCGGCGGAGG 
CGTGGGGGGC CTGGGCCTGG CATGATGTGT GGAGCTGTCA GTGACCTCCT GAGTGACACG 
GAAGAAGACG ATAAGTGCTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cacna1e ( XM_011247909.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011246211.1 Mus musculus calcium channel, voltage-dependent, R type, alpha 1E subunit (Cacna1e), transcript variant X2, mRNA.

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CloneID	OMu67260
Clone ID Related Accession (Same CDS sequence)	XM_017312578.1 , XM_030243926.1 , XM_017312594.1 , XM_030243929.1 , XM_011247909.2 , XM_017312578.2 , XM_011247909.3
Accession Version	XM_011247909.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7401bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	voltage-dependent R-type calcium channel subunit alpha-1E isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_011247909.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGCCCG AGATGCGTGG ACTGAGCCCA GGCCGAAGGC TGCCCGAGCC CACCCTGTGC 
CCGGCTACAG GAGGGAGCCC TGAGCCCGGG GCTGCTGGCA GGCGCCGCCA CGTCCTGGCT 
CTGCCTGATG AGGAGGCATG GCGGCCGCAG CAGTGCGCCC TGCTCGAGGC CAATGCCTCC 
GACGAGGTGG GCATGTTGCC TGCTTCCCCG CGCTCCGCGG TGGCCGGCTT CGACAACTTC 
TTCCCGGTGC AGGAAGGCGA GGGCCCGGGC TGGGAGGGCA GCACCACGCT GGATGCAGGC 
TCCAGCCCCT TCCTTCCTGT GAGCCCAGAG GTCATGAAGC GGAGGCGAGG GGGGCTCATC 
GAGCAGCGGG ACATCATCAA GGCCCACGAG GCACACAAGA TGCAGAGCAC CCCGCAGGCC 
CGGCGCAAGG AGTGGGAGAT GGCTCGCTTC GGGGAGGCGG TGGTCGTTGG CAGGCCAGGC 
TCAGGCGATG GAGACTCGGA CCAGAGCAGG AACCGACAAG GAACCCCCGT CCCGGCCTCG 
GGGCCGGCGG CCGCCTACAA GCAGTCAAAA GCGCAGAGGG CGCGGACTAT GGCTTTGTAC 
AACCCCATAC CAGTCCGGCA GAACTGTTTC ACGGTCAACA GATCCCTGTT CATCTTCGGA 
GAAGATAACA TTGTCAGGAA ATACGCCAAG AAGCTCATCG ATTGGCCGCC ATTTGAGTAC 
ATGATCCTGG CCACCATCAT TGCCAACTGC ATCGTCTTGG CCCTGGAGCA GCATCTTCCT 
GAGGATGACA AGACCCCAAT GTCTCGAAGA CTGGAGAAGA CAGAACCATA TTTCATTGGG 
ATCTTCTGCT TTGAAGCTGG GATCAAAATT GTGGCTCTAG GGTTCATCTT CCATAAGGGT 
TCATACCTCC GTAATGGCTG GAATGTCATG GACTTCATCG TGGTCCTTAG TGGCATCCTG 
GCCACTGCGG GAACCCACTT CAACACCCAC GTGGACCTGC GGACCCTCCG GGCTGTGCGT 
GTCCTGAGGC CTTTAAAGCT GGTATCAGGA ATACCTAGCC TGCAGATCGT GCTGAAGTCT 
ATCATGAAGG CCATGGTGCC TCTTCTGCAG ATTGGCCTTC TGCTCTTCTT TGCCATCCTC 
ATGTTTGCTA TCATTGGCTT GGAGTTCTAC AGTGGCAAGT TGCATCGAGC ATGCTTCATG 
AACAATTCAG GTATTCTAGA GGGGTTTGAT CCTCCTCACC CGTGTGGTGT GCAAGGCTGC 
CCGGCTGGTT ATGAATGTAA GGACTGGATC GGCCCCAATG ACGGGATCAC CCAGTTTGAC 
AACATCCTTT TTGCCGTGCT GACTGTCTTC CAATGCATCA CTATGGAAGG GTGGACCACT 
GTGCTGTACA ATACCAATGA TGCCTTAGGA GCCACCTGGA ACTGGCTGTA CTTCATCCCT 
CTCATCATCA TCGGATCCTT CTTTGTTCTC AACCTCGTCC TGGGAGTGCT TTCCGGGGAA 
TTTGCCAAAG AAAGAGAGAG AGTGGAGAAC CGAAGAGCGT TCATGAAGCT CCGGCGCCAG 
CAACAGATTG AGCGAGAATT GAACGGCTAC CGTGCCTGGA TAGACAAAGC AGAGGAAGTC 
ATGCTTGCTG AAGAAAATAA AAACTCGGGA ACATCAGCCT TGGAAGTGCT TCGAAGGGCA 
ACCATCAAAA GGAGCCGGAC GGAGGCCATG ACCCGAGACT CCAGCGATGA GCACTGCGTT 
GATATCTCTT CAGTGGGCAC ACCTCTTGCC AGAGCCAGTA TCAAGAGCAC AAAAGTAGAT 
GGGGCCTCCT ATTTCCGGCA CAAGGAACGG CTTCTGCGCA TCTCTATCCG CCACATGGTC 
AAGTCCCAAG TCTTTTACTG GATCGTCCTG AGTGTTGTGG CTCTTAATAC TGCCTGTGTG 
GCCATTGTTC ATCACAACCA GCCACAGTGG CTCACTCACC TCCTCTACTA TGCAGAGTTT 
TTGTTTCTGG GACTCTTCCT GTTGGAGATG TCCCTGAAGA TGTATGGCAT GGGGCCACGC 
CTCTATTTTC ACTCATCCTT CAACTGCTTT GATTTTGGGG TCACAGTGGG CAGCATCTTT 
GAGGTTGTCT GGGCAATCTT CAGACCTGGC ACATCTTTTG GAATCAGTGT CTTACGAGCT 
CTCCGGCTCC TAAGGATATT TAAAATAACC AAGTATTGGG CATCCCTCCG GAATCTGGTT 
GTCTCCTTGA TGAGCTCCAT GAAGTCTATC ATCAGCTTAC TCTTCCTTCT CTTCCTCTTC 
ATCGTTGTCT TTGCTCTCCT GGGAATGCAG CTCTTTGGAG GCAGGTTTAA CTTTAATGAT 
GGGACTCCTT CAGCTAATTT TGACACCTTC CCTGCAGCTA TCATGACAGT GTTCCAGATC 
CTGACAGGGG AAGACTGGAA TGAGGTGATG TACAATGGCA TTCGCTCCCA GGGTGGGGTC 
AGCTCAGGCA TGTGGTCTGC CATCTACTTC ATTGTGCTCA CCTTGTTTGG AAACTACACG 
CTGCTAAATG TATTCTTGGC CATCGCTGTG GACAATCTGG CCAATGCCCA GGAACTGACC 
AAGGATGAGC AGGAGGAAGA AGAAGCCTTC AACCAGAAAC ATGCACTGCA GAAGGCCAAG 
GAGGTCAGCC CGATGTCTGC TCCCAACATG CCTTCCATTG AAAGAGACAG AAGGAGAAGA 
CACCACATGT CGATGTGGGA GCCACGCAGC AGCCACCTGA GGGAGCGGAG GCGCCGGCAC 
CACATGTCTG TGTGGGAACA ACGCACCAGC CAGCTGAGGA GGCACATGCA AATGTCTAGC 
CAAGAAGCCC TCAACAAAGA GGAGGCCCCG CCAATGAACC CCCTCAATCC ACTCAACCCA 
CTGAGTCCTC TCAACCCCCT CAATGCTCAC CCCAGCCTTT ATCGGAGGCC CAGGCCCATT 
GAGGGCCTAG CCCTAGGTCT GGGTCTTGAA AAGTGTGAAG AAGAACGCAT CAGTCGTGGG 
GGCTCCCTCA AGGGGGACAT AGGGGGCCTG ACTAGCGCCC TGGACAACCA GAGGAGCCCC 
TTGTCCTTGG GCAAACGGGA GCCACCGTGG CTGCCTAGAT CCTGTCATGG AAACTGTGAC 
CCAATCCAGC AGGAGGCTGG GGGAGGAGAG ACTGTGGTGA CTTTTGAGGA CCGGGCCAGG 
CACAGGCAGA GTCAGAGGCG AAGCCGGCAT CGCCGAGTCA GGACTGAGGG CAAGGACTCT 
GCCTCTGCAT CCCGAAGCAG GTCTGCCAGC CAGGAGCGGA GTCTGGATGA AGGGGTGTCC 
GTTGAGGGGG AGAAGGAACA TGAGCCTCAC AGCAGCCACA GGAGCAAAGA GCCGACCATC 
CACGAAGAGG AGAGAACCCA GGACTTAAGG AGGACCAACA GTCTGATGGT CCCCAGGGGC 
TCTGGACTGG TAGGGGCTCT TGATGAAGCA GAAACACCTC TAGTCCAGCC CCAACCTGAG 
CTGGAAGTGG GGAAGGATGC AGCTCTGACA GAGCAGGAGG CTGAAGGCAG CAGTGAACAA 
GCCCTTCTTG GGGACGTACA GCTGGATGTG GGCCGGGGCA TTAGCCAGAG TGAACCGGAT 
CTCTCCTGCA TGACAGCCAA CATGGACAAG GCTACCACGG AGAGCACCAG TGTCACGGTC 
GCCATCCCTG ATGTGGACCC CTTGGTGGAC TCGACAGTGG TGAACATTAG CAACAAGACG 
GATGGAGAAG CCAGTCCCTT GAAGGAGGCA GAGACTAAAG AGGAAGAAGA AGAGGTAGAG 
AAGAAGAAGA AGCAGAAGAA GGAGAAGCGA GAGACTGGAA AAGCCATGGT GCCCCATAGC 
TCTATGTTCA TCTTCAGCAC CACTAACCCG ATCCGCAGGG CCTGCCACTA CATTGTGAAC 
CTACGCTACT TTGAAATGTG CATTCTCTTG GTGATTGCGG CCAGCAGCAT TGCCCTGGCA 
GCCGAGGACC CTGTACTAAC TAACTCGGAG CGTAACAAAG TCCTGAGATA TTTCGACTAT 
GTTTTCACAG GAGTGTTTAC CTTTGAGATG GTTATAAAGA TGATAGACCA AGGCTTGATC 
CTGCAGGATG GATCATACTT CAGAGACCTC TGGAACATCC TGGACTTTGT AGTAGTGGTT 
GGTGCATTGG TAGCATTTGC TCTGGCGAAC GCTTTGGGAA CCAACAAGGG GAGGGATATC 
AAGACTATCA AGTCTCTGAG GGTACTTCGA GTTCTGCGGC CACTGAAAAC CATCAAGCGC 
TTGCCCAAGC TGAAGGCTGT GTTTGACTGC GTGGTGACCT CCTTGAAGAA TGTCTTCAAC 
ATACTCATTG TCTACAAGCT CTTCATGTTC ATCTTTGCCG TCATCGCTGT TCAGCTATTC 
AAAGGAAAGT TCTTTTATTG CACTGACAGT TCCAAGGACA CAGAGAAGGA GTGCATAGGC 
AACTATGTAG ACCACGAGAA AAACAAGATG GAGGTGAAGG GCCGGGAATG GAAGCGTCAT 
GAATTCCACT ATGACAACAT CATCTGGGCC CTGCTTACCC TCTTCACAGT CTCTACAGGG 
GAAGGATGGC CTCAGGTTCT GCAGCACTCT GTAGATGTGA CAGAGGAGGA CAGAGGCCCA 
AGTCGCAGCA ACCGCATGGA GATGTCCATC TTTTATGTGG TCTACTTTGT GGTCTTCCCT 
TTCTTCTTTG TCAATATCTT CGTGGCTCTC ATTATCATCA CCTTCCAGGA GCAAGGGGAC 
AAGATGATGG AGGAGTGTAG CCTGGAGAAG AATGAGCGGG CATGCATCGA CTTCGCCATT 
AGTGCCAAAC CTCTCACCCG CTACATGCCA CAGAACAGGC ACACCTTCCA GTACCGTGTG 
TGGCACTTTG TAGTCTCCCC ATCCTTTGAG TATACCATCA TGGCCATGAT TGCCTTGAAC 
ACTGTCGTGC TGATGATGAA GTACTACTCT GCTCCTTGCA CCTATGAGCT CGCCTTAAAG 
TACCTGAACA TCGCCTTCAC TATGGTGTTT TCCCTGGAGT GTGTCCTAAA GGTCATTGCT 
TTCGGCTTCC TGAACTATTT CCGAGATACC TGGAACATCT TTGACTTCAT CACGGTGATC 
GGCAGTATCA CAGAAATTAT CCTGACAGAT AGCAAGCTGG TGAACACCAG TGGCTTCAAT 
ATGAGCTTTC TGAAGCTCTT CCGAGCTGCA CGGCTCATAA AGCTTCTGAG ACAGGGCTAC 
ACCATCCGCA TCTTACTGTG GACCTTCGTG CAGTCCTTTA AGGCTCTTCC CTACGTCTGC 
CTTCTCATTG CCATGCTTTT CTTCATCTAC GCCATCATTG GGATGCAGGT GTTTGGAAAC 
ATAAAGTTAG ATGAGGAGAG TCACATCAAC CGGCACAACA ACTTCCGGAG TTTCTTTGGA 
TCCCTCATGC TGCTTTTCAG GAGTGCCACG GGGGAGGCTT GGCAGGAGAT TATGCTGTCC 
TGTCTCGGTG AGAAGGGTTG TGAGCCTGAC ACCACTGCAC CCTCAGGGCA GAATGAGAGT 
GAGCGCTGTG GCACTGACCT GGCCTACGTT TATTTTGTCT CCTTCATCTT CTTCTGCTCC 
TTCCTGATGC TCAACCTGTT CGTGGCTGTC ATCATGGACA ACTTTGAGTA CCTGACTCGA 
GATTCCTCCA TCCTGGGGCC TCACCACTTA GATGAGTTTG TCCGTGTCTG GGCAGAATAT 
GACAGAGCAG CATGTGGCCG CATCCATTAC ACTGAGATGT ATGAAATGCT GACTCTCATG 
TCACCACCGC TAGGCCTCGG CAAGAGATGT CCCTCCAAAG TGGCCTATAA GAGGCTGGTC 
CTGATGAACA TGCCAGTGGC TGAGGACATG ACAGTCCACT TCACCTCCAC ACTTATGGCT 
CTGATCCGGA CAGCTCTGGA CATTAAAATC GCCAAAGGTG GTGCAGACAG ACAGCAGCTA 
GACTCGGAGC TGCAAAAAGA GACCCTGGCC ATTTGGCCTC ACCTGTCCCA AAAGATGTTG 
GATCTGCTTG TGCCCATGCC CAAAGCCTCT GACCTGACTG TGGGCAAGAT CTATGCAGCA 
ATGATGATCA TGGACTACTA TAAACAGAGT AAGGTAAAGA AACAGAGACA GCAGCTGGAA 
GAACAGAAAA ATGCACCCAT GTTCCAGCGC ATGGAGCCCT CATCACTGCC TCAGGAGATC 
ATTGCTAATG CCAAAGCCCT GCCTTATCTC CAGCAGGACC CTGTTTCTGG CCTGAGTGGT 
CGGAGTGGAT ACCCTTCAAT GAGTCCACTC TCCCCTCAAG AAATATTCCA GTTGGCTTGT 
ATGGACCCAG CGGATGATGG ACAATTCCAG GAACAACAAT CTCTGGTGAA TGCACAAGAG 
CCAGAGGTTA GTGAATTAAA AAGTGTGCAG TCCTCTAACC ATGGCATCTA CCTTCCTCCG 
GACACCCAGG AGCATGCGGG ATCTGGGAGG GCATCCTCTA TGCCACGTCT GACTATGGAT 
CCCCAGGTGG TAACAGACCC TAGCTCTATG CGACGTTCAT TTTCTACAAT TCGGGATAAG 
CGTTCAAATT CCTCCTGGTT GGAGGAGTTC TCCATGGAAC GCAGCAGTGA AAACACTTAC 
AAGTCGCGGC GCAGAAGTTA CCATTCCTCC CTGAGGCTGT CAGCACACCG CCTGAATTCT 
GACTCTGGTC ACAAGTCTGA CACTCACCGA TCAGGAGGCA GGGAGAGAGG CCGATCCAAA 
GAACGAAAGC ACCTCCTCTC TCCAGATGTC TCCCGCTGCA ATTCTGAAGA GCGAGGGACC 
CAGGCCGACT GGGAGTCCCC AGAACGTCGC CAGTCCAGGT CACCCAGTGA AGGAAGGTCA 
CAGACCCCCA ATAGACAGGG TACCGGTTCC CTAAGTGAGA GCTCCATCCC CTCTATTTCC 
GACACCAGCA CCCCAAGACG AAGCCGTCGC CAGCTCCCAC CTGTGCCACC AAAGCCTCGT 
CCCCTCCTCT CCTACAGCTC CCTGATGAGA CACACTGGCG GCATCTCTCC ACCTCCTGAT 
GGAAGTGAGG GTGGATCCCC ACTGGCCTCT CAAGCTCTGG AGAGCAACAG CGCTTGCCTG 
ACCGAGTCTT CCAACTCCTT GCACCCCCAG CAGGGCCAGC ATCCTTCCCC ACAGCACTAC 
ATCTCCGAGC CCTATCTGGC CCTCCATGAA GACTCCCACG CCTCAGACTG TGGCGAGGAG 
GAGACACTCA CCTTTGAGGC AGCCGTAGCC ACTAGCTTGG GCCGGTCCAA TACCATTGGC 
TCTGCGCCAC CCCTACGGCA CAGCTGGCAG ATGCCTAATG GGCACTATCG GCGGCGGAGG 
CGTGGGGGGC CTGGGCCTGG CATGATGTGT GGAGCTGTCA GTGACCTCCT GAGTGACACG 
GAAGAAGACG ATAAGTGCTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011246211.1
CDS	522..7922
Translation	MVPEMRGLSPGRRLPEPTLCPATGGSPEPGAAGRRRHVLALPDEEAWRPQQCALLEANASDEVGMLPASPRSAVAGFDNFFPVQEGEGPGWEGSTTLDAGSSPFLPVSPEVMKRRRGGLIEQRDIIKAHEAHKMQSTPQARRKEWEMARFGEAVVVGRPGSGDGDSDQSRNRQGTPVPASGPAAAYKQSKAQRARTMALYNPIPVRQNCFTVNRSLFIFGEDNIVRKYAKKLIDWPPFEYMILATIIANCIVLALEQHLPEDDKTPMSRRLEKTEPYFIGIFCFEAGIKIVALGFIFHKGSYLRNGWNVMDFIVVLSGILATAGTHFNTHVDLRTLRAVRVLRPLKLVSGIPSLQIVLKSIMKAMVPLLQIGLLLFFAILMFAIIGLEFYSGKLHRACFMNNSGILEGFDPPHPCGVQGCPAGYECKDWIGPNDGITQFDNILFAVLTVFQCITMEGWTTVLYNTNDALGATWNWLYFIPLIIIGSFFVLNLVLGVLSGEFAKERERVENRRAFMKLRRQQQIERELNGYRAWIDKAEEVMLAEENKNSGTSALEVLRRATIKRSRTEAMTRDSSDEHCVDISSVGTPLARASIKSTKVDGASYFRHKERLLRISIRHMVKSQVFYWIVLSVVALNTACVAIVHHNQPQWLTHLLYYAEFLFLGLFLLEMSLKMYGMGPRLYFHSSFNCFDFGVTVGSIFEVVWAIFRPGTSFGISVLRALRLLRIFKITKYWASLRNLVVSLMSSMKSIISLLFLLFLFIVVFALLGMQLFGGRFNFNDGTPSANFDTFPAAIMTVFQILTGEDWNEVMYNGIRSQGGVSSGMWSAIYFIVLTLFGNYTLLNVFLAIAVDNLANAQELTKDEQEEEEAFNQKHALQKAKEVSPMSAPNMPSIERDRRRRHHMSMWEPRSSHLRERRRRHHMSVWEQRTSQLRRHMQMSSQEALNKEEAPPMNPLNPLNPLSPLNPLNAHPSLYRRPRPIEGLALGLGLEKCEEERISRGGSLKGDIGGLTSALDNQRSPLSLGKREPPWLPRSCHGNCDPIQQEAGGGETVVTFEDRARHRQSQRRSRHRRVRTEGKDSASASRSRSASQERSLDEGVSVEGEKEHEPHSSHRSKEPTIHEEERTQDLRRTNSLMVPRGSGLVGALDEAETPLVQPQPELEVGKDAALTEQEAEGSSEQALLGDVQLDVGRGISQSEPDLSCMTANMDKATTESTSVTVAIPDVDPLVDSTVVNISNKTDGEASPLKEAETKEEEEEVEKKKKQKKEKRETGKAMVPHSSMFIFSTTNPIRRACHYIVNLRYFEMCILLVIAASSIALAAEDPVLTNSERNKVLRYFDYVFTGVFTFEMVIKMIDQGLILQDGSYFRDLWNILDFVVVVGALVAFALANALGTNKGRDIKTIKSLRVLRVLRPLKTIKRLPKLKAVFDCVVTSLKNVFNILIVYKLFMFIFAVIAVQLFKGKFFYCTDSSKDTEKECIGNYVDHEKNKMEVKGREWKRHEFHYDNIIWALLTLFTVSTGEGWPQVLQHSVDVTEEDRGPSRSNRMEMSIFYVVYFVVFPFFFVNIFVALIIITFQEQGDKMMEECSLEKNERACIDFAISAKPLTRYMPQNRHTFQYRVWHFVVSPSFEYTIMAMIALNTVVLMMKYYSAPCTYELALKYLNIAFTMVFSLECVLKVIAFGFLNYFRDTWNIFDFITVIGSITEIILTDSKLVNTSGFNMSFLKLFRAARLIKLLRQGYTIRILLWTFVQSFKALPYVCLLIAMLFFIYAIIGMQVFGNIKLDEESHINRHNNFRSFFGSLMLLFRSATGEAWQEIMLSCLGEKGCEPDTTAPSGQNESERCGTDLAYVYFVSFIFFCSFLMLNLFVAVIMDNFEYLTRDSSILGPHHLDEFVRVWAEYDRAACGRIHYTEMYEMLTLMSPPLGLGKRCPSKVAYKRLVLMNMPVAEDMTVHFTSTLMALIRTALDIKIAKGGADRQQLDSELQKETLAIWPHLSQKMLDLLVPMPKASDLTVGKIYAAMMIMDYYKQSKVKKQRQQLEEQKNAPMFQRMEPSSLPQEIIANAKALPYLQQDPVSGLSGRSGYPSMSPLSPQEIFQLACMDPADDGQFQEQQSLVNAQEPEVSELKSVQSSNHGIYLPPDTQEHAGSGRASSMPRLTMDPQVVTDPSSMRRSFSTIRDKRSNSSWLEEFSMERSSENTYKSRRRSYHSSLRLSAHRLNSDSGHKSDTHRSGGRERGRSKERKHLLSPDVSRCNSEERGTQADWESPERRQSRSPSEGRSQTPNRQGTGSLSESSIPSISDTSTPRRSRRQLPPVPPKPRPLLSYSSLMRHTGGISPPPDGSEGGSPLASQALESNSACLTESSNSLHPQQGQHPSPQHYISEPYLALHEDSHASDCGEEETLTFEAAVATSLGRSNTIGSAPPLRHSWQMPNGHYRRRRRGGPGPGMMCGAVSDLLSDTEEDDKC

Target ORF information:

RefSeq Version	XM_011247909.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus calcium channel, voltage-dependent, R type, alpha 1E subunit (Cacna1e), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011247909.3

ORF Insert Sequence:

ATGGTGCCCG AGATGCGTGG ACTGAGCCCA GGCCGAAGGC TGCCCGAGCC CACCCTGTGC 
CCGGCTACAG GAGGGAGCCC TGAGCCCGGG GCTGCTGGCA GGCGCCGCCA CGTCCTGGCT 
CTGCCTGATG AGGAGGCATG GCGGCCGCAG CAGTGCGCCC TGCTCGAGGC CAATGCCTCC 
GACGAGGTGG GCATGTTGCC TGCTTCCCCG CGCTCCGCGG TGGCCGGCTT CGACAACTTC 
TTCCCGGTGC AGGAAGGCGA GGGCCCGGGC TGGGAGGGCA GCACCACGCT GGATGCAGGC 
TCCAGCCCCT TCCTTCCTGT GAGCCCAGAG GTCATGAAGC GGAGGCGAGG GGGGCTCATC 
GAGCAGCGGG ACATCATCAA GGCCCACGAG GCACACAAGA TGCAGAGCAC CCCGCAGGCC 
CGGCGCAAGG AGTGGGAGAT GGCTCGCTTC GGGGAGGCGG TGGTCGTTGG CAGGCCAGGC 
TCAGGCGATG GAGACTCGGA CCAGAGCAGG AACCGACAAG GAACCCCCGT CCCGGCCTCG 
GGGCCGGCGG CCGCCTACAA GCAGTCAAAA GCGCAGAGGG CGCGGACTAT GGCTTTGTAC 
AACCCCATAC CAGTCCGGCA GAACTGTTTC ACGGTCAACA GATCCCTGTT CATCTTCGGA 
GAAGATAACA TTGTCAGGAA ATACGCCAAG AAGCTCATCG ATTGGCCGCC ATTTGAGTAC 
ATGATCCTGG CCACCATCAT TGCCAACTGC ATCGTCTTGG CCCTGGAGCA GCATCTTCCT 
GAGGATGACA AGACCCCAAT GTCTCGAAGA CTGGAGAAGA CAGAACCATA TTTCATTGGG 
ATCTTCTGCT TTGAAGCTGG GATCAAAATT GTGGCTCTAG GGTTCATCTT CCATAAGGGT 
TCATACCTCC GTAATGGCTG GAATGTCATG GACTTCATCG TGGTCCTTAG TGGCATCCTG 
GCCACTGCGG GAACCCACTT CAACACCCAC GTGGACCTGC GGACCCTCCG GGCTGTGCGT 
GTCCTGAGGC CTTTAAAGCT GGTATCAGGA ATACCTAGCC TGCAGATCGT GCTGAAGTCT 
ATCATGAAGG CCATGGTGCC TCTTCTGCAG ATTGGCCTTC TGCTCTTCTT TGCCATCCTC 
ATGTTTGCTA TCATTGGCTT GGAGTTCTAC AGTGGCAAGT TGCATCGAGC ATGCTTCATG 
AACAATTCAG GTATTCTAGA GGGGTTTGAT CCTCCTCACC CGTGTGGTGT GCAAGGCTGC 
CCGGCTGGTT ATGAATGTAA GGACTGGATC GGCCCCAATG ACGGGATCAC CCAGTTTGAC 
AACATCCTTT TTGCCGTGCT GACTGTCTTC CAATGCATCA CTATGGAAGG GTGGACCACT 
GTGCTGTACA ATACCAATGA TGCCTTAGGA GCCACCTGGA ACTGGCTGTA CTTCATCCCT 
CTCATCATCA TCGGATCCTT CTTTGTTCTC AACCTCGTCC TGGGAGTGCT TTCCGGGGAA 
TTTGCCAAAG AAAGAGAGAG AGTGGAGAAC CGAAGAGCGT TCATGAAGCT CCGGCGCCAG 
CAACAGATTG AGCGAGAATT GAACGGCTAC CGTGCCTGGA TAGACAAAGC AGAGGAAGTC 
ATGCTTGCTG AAGAAAATAA AAACTCGGGA ACATCAGCCT TGGAAGTGCT TCGAAGGGCA 
ACCATCAAAA GGAGCCGGAC GGAGGCCATG ACCCGAGACT CCAGCGATGA GCACTGCGTT 
GATATCTCTT CAGTGGGCAC ACCTCTTGCC AGAGCCAGTA TCAAGAGCAC AAAAGTAGAT 
GGGGCCTCCT ATTTCCGGCA CAAGGAACGG CTTCTGCGCA TCTCTATCCG CCACATGGTC 
AAGTCCCAAG TCTTTTACTG GATCGTCCTG AGTGTTGTGG CTCTTAATAC TGCCTGTGTG 
GCCATTGTTC ATCACAACCA GCCACAGTGG CTCACTCACC TCCTCTACTA TGCAGAGTTT 
TTGTTTCTGG GACTCTTCCT GTTGGAGATG TCCCTGAAGA TGTATGGCAT GGGGCCACGC 
CTCTATTTTC ACTCATCCTT CAACTGCTTT GATTTTGGGG TCACAGTGGG CAGCATCTTT 
GAGGTTGTCT GGGCAATCTT CAGACCTGGC ACATCTTTTG GAATCAGTGT CTTACGAGCT 
CTCCGGCTCC TAAGGATATT TAAAATAACC AAGTATTGGG CATCCCTCCG GAATCTGGTT 
GTCTCCTTGA TGAGCTCCAT GAAGTCTATC ATCAGCTTAC TCTTCCTTCT CTTCCTCTTC 
ATCGTTGTCT TTGCTCTCCT GGGAATGCAG CTCTTTGGAG GCAGGTTTAA CTTTAATGAT 
GGGACTCCTT CAGCTAATTT TGACACCTTC CCTGCAGCTA TCATGACAGT GTTCCAGATC 
CTGACAGGGG AAGACTGGAA TGAGGTGATG TACAATGGCA TTCGCTCCCA GGGTGGGGTC 
AGCTCAGGCA TGTGGTCTGC CATCTACTTC ATTGTGCTCA CCTTGTTTGG AAACTACACG 
CTGCTAAATG TATTCTTGGC CATCGCTGTG GACAATCTGG CCAATGCCCA GGAACTGACC 
AAGGATGAGC AGGAGGAAGA AGAAGCCTTC AACCAGAAAC ATGCACTGCA GAAGGCCAAG 
GAGGTCAGCC CGATGTCTGC TCCCAACATG CCTTCCATTG AAAGAGACAG AAGGAGAAGA 
CACCACATGT CGATGTGGGA GCCACGCAGC AGCCACCTGA GGGAGCGGAG GCGCCGGCAC 
CACATGTCTG TGTGGGAACA ACGCACCAGC CAGCTGAGGA GGCACATGCA AATGTCTAGC 
CAAGAAGCCC TCAACAAAGA GGAGGCCCCG CCAATGAACC CCCTCAATCC ACTCAACCCA 
CTGAGTCCTC TCAACCCCCT CAATGCTCAC CCCAGCCTTT ATCGGAGGCC CAGGCCCATT 
GAGGGCCTAG CCCTAGGTCT GGGTCTTGAA AAGTGTGAAG AAGAACGCAT CAGTCGTGGG 
GGCTCCCTCA AGGGGGACAT AGGGGGCCTG ACTAGCGCCC TGGACAACCA GAGGAGCCCC 
TTGTCCTTGG GCAAACGGGA GCCACCGTGG CTGCCTAGAT CCTGTCATGG AAACTGTGAC 
CCAATCCAGC AGGAGGCTGG GGGAGGAGAG ACTGTGGTGA CTTTTGAGGA CCGGGCCAGG 
CACAGGCAGA GTCAGAGGCG AAGCCGGCAT CGCCGAGTCA GGACTGAGGG CAAGGACTCT 
GCCTCTGCAT CCCGAAGCAG GTCTGCCAGC CAGGAGCGGA GTCTGGATGA AGGGGTGTCC 
GTTGAGGGGG AGAAGGAACA TGAGCCTCAC AGCAGCCACA GGAGCAAAGA GCCGACCATC 
CACGAAGAGG AGAGAACCCA GGACTTAAGG AGGACCAACA GTCTGATGGT CCCCAGGGGC 
TCTGGACTGG TAGGGGCTCT TGATGAAGCA GAAACACCTC TAGTCCAGCC CCAACCTGAG 
CTGGAAGTGG GGAAGGATGC AGCTCTGACA GAGCAGGAGG CTGAAGGCAG CAGTGAACAA 
GCCCTTCTTG GGGACGTACA GCTGGATGTG GGCCGGGGCA TTAGCCAGAG TGAACCGGAT 
CTCTCCTGCA TGACAGCCAA CATGGACAAG GCTACCACGG AGAGCACCAG TGTCACGGTC 
GCCATCCCTG ATGTGGACCC CTTGGTGGAC TCGACAGTGG TGAACATTAG CAACAAGACG 
GATGGAGAAG CCAGTCCCTT GAAGGAGGCA GAGACTAAAG AGGAAGAAGA AGAGGTAGAG 
AAGAAGAAGA AGCAGAAGAA GGAGAAGCGA GAGACTGGAA AAGCCATGGT GCCCCATAGC 
TCTATGTTCA TCTTCAGCAC CACTAACCCG ATCCGCAGGG CCTGCCACTA CATTGTGAAC 
CTACGCTACT TTGAAATGTG CATTCTCTTG GTGATTGCGG CCAGCAGCAT TGCCCTGGCA 
GCCGAGGACC CTGTACTAAC TAACTCGGAG CGTAACAAAG TCCTGAGATA TTTCGACTAT 
GTTTTCACAG GAGTGTTTAC CTTTGAGATG GTTATAAAGA TGATAGACCA AGGCTTGATC 
CTGCAGGATG GATCATACTT CAGAGACCTC TGGAACATCC TGGACTTTGT AGTAGTGGTT 
GGTGCATTGG TAGCATTTGC TCTGGCGAAC GCTTTGGGAA CCAACAAGGG GAGGGATATC 
AAGACTATCA AGTCTCTGAG GGTACTTCGA GTTCTGCGGC CACTGAAAAC CATCAAGCGC 
TTGCCCAAGC TGAAGGCTGT GTTTGACTGC GTGGTGACCT CCTTGAAGAA TGTCTTCAAC 
ATACTCATTG TCTACAAGCT CTTCATGTTC ATCTTTGCCG TCATCGCTGT TCAGCTATTC 
AAAGGAAAGT TCTTTTATTG CACTGACAGT TCCAAGGACA CAGAGAAGGA GTGCATAGGC 
AACTATGTAG ACCACGAGAA AAACAAGATG GAGGTGAAGG GCCGGGAATG GAAGCGTCAT 
GAATTCCACT ATGACAACAT CATCTGGGCC CTGCTTACCC TCTTCACAGT CTCTACAGGG 
GAAGGATGGC CTCAGGTTCT GCAGCACTCT GTAGATGTGA CAGAGGAGGA CAGAGGCCCA 
AGTCGCAGCA ACCGCATGGA GATGTCCATC TTTTATGTGG TCTACTTTGT GGTCTTCCCT 
TTCTTCTTTG TCAATATCTT CGTGGCTCTC ATTATCATCA CCTTCCAGGA GCAAGGGGAC 
AAGATGATGG AGGAGTGTAG CCTGGAGAAG AATGAGCGGG CATGCATCGA CTTCGCCATT 
AGTGCCAAAC CTCTCACCCG CTACATGCCA CAGAACAGGC ACACCTTCCA GTACCGTGTG 
TGGCACTTTG TAGTCTCCCC ATCCTTTGAG TATACCATCA TGGCCATGAT TGCCTTGAAC 
ACTGTCGTGC TGATGATGAA GTACTACTCT GCTCCTTGCA CCTATGAGCT CGCCTTAAAG 
TACCTGAACA TCGCCTTCAC TATGGTGTTT TCCCTGGAGT GTGTCCTAAA GGTCATTGCT 
TTCGGCTTCC TGAACTATTT CCGAGATACC TGGAACATCT TTGACTTCAT CACGGTGATC 
GGCAGTATCA CAGAAATTAT CCTGACAGAT AGCAAGCTGG TGAACACCAG TGGCTTCAAT 
ATGAGCTTTC TGAAGCTCTT CCGAGCTGCA CGGCTCATAA AGCTTCTGAG ACAGGGCTAC 
ACCATCCGCA TCTTACTGTG GACCTTCGTG CAGTCCTTTA AGGCTCTTCC CTACGTCTGC 
CTTCTCATTG CCATGCTTTT CTTCATCTAC GCCATCATTG GGATGCAGGT GTTTGGAAAC 
ATAAAGTTAG ATGAGGAGAG TCACATCAAC CGGCACAACA ACTTCCGGAG TTTCTTTGGA 
TCCCTCATGC TGCTTTTCAG GAGTGCCACG GGGGAGGCTT GGCAGGAGAT TATGCTGTCC 
TGTCTCGGTG AGAAGGGTTG TGAGCCTGAC ACCACTGCAC CCTCAGGGCA GAATGAGAGT 
GAGCGCTGTG GCACTGACCT GGCCTACGTT TATTTTGTCT CCTTCATCTT CTTCTGCTCC 
TTCCTGATGC TCAACCTGTT CGTGGCTGTC ATCATGGACA ACTTTGAGTA CCTGACTCGA 
GATTCCTCCA TCCTGGGGCC TCACCACTTA GATGAGTTTG TCCGTGTCTG GGCAGAATAT 
GACAGAGCAG CATGTGGCCG CATCCATTAC ACTGAGATGT ATGAAATGCT GACTCTCATG 
TCACCACCGC TAGGCCTCGG CAAGAGATGT CCCTCCAAAG TGGCCTATAA GAGGCTGGTC 
CTGATGAACA TGCCAGTGGC TGAGGACATG ACAGTCCACT TCACCTCCAC ACTTATGGCT 
CTGATCCGGA CAGCTCTGGA CATTAAAATC GCCAAAGGTG GTGCAGACAG ACAGCAGCTA 
GACTCGGAGC TGCAAAAAGA GACCCTGGCC ATTTGGCCTC ACCTGTCCCA AAAGATGTTG 
GATCTGCTTG TGCCCATGCC CAAAGCCTCT GACCTGACTG TGGGCAAGAT CTATGCAGCA 
ATGATGATCA TGGACTACTA TAAACAGAGT AAGGTAAAGA AACAGAGACA GCAGCTGGAA 
GAACAGAAAA ATGCACCCAT GTTCCAGCGC ATGGAGCCCT CATCACTGCC TCAGGAGATC 
ATTGCTAATG CCAAAGCCCT GCCTTATCTC CAGCAGGACC CTGTTTCTGG CCTGAGTGGT 
CGGAGTGGAT ACCCTTCAAT GAGTCCACTC TCCCCTCAAG AAATATTCCA GTTGGCTTGT 
ATGGACCCAG CGGATGATGG ACAATTCCAG GAACAACAAT CTCTGGTGAA TGCACAAGAG 
CCAGAGGTTA GTGAATTAAA AAGTGTGCAG TCCTCTAACC ATGGCATCTA CCTTCCTCCG 
GACACCCAGG AGCATGCGGG ATCTGGGAGG GCATCCTCTA TGCCACGTCT GACTATGGAT 
CCCCAGGTGG TAACAGACCC TAGCTCTATG CGACGTTCAT TTTCTACAAT TCGGGATAAG 
CGTTCAAATT CCTCCTGGTT GGAGGAGTTC TCCATGGAAC GCAGCAGTGA AAACACTTAC 
AAGTCGCGGC GCAGAAGTTA CCATTCCTCC CTGAGGCTGT CAGCACACCG CCTGAATTCT 
GACTCTGGTC ACAAGTCTGA CACTCACCGA TCAGGAGGCA GGGAGAGAGG CCGATCCAAA 
GAACGAAAGC ACCTCCTCTC TCCAGATGTC TCCCGCTGCA ATTCTGAAGA GCGAGGGACC 
CAGGCCGACT GGGAGTCCCC AGAACGTCGC CAGTCCAGGT CACCCAGTGA AGGAAGGTCA 
CAGACCCCCA ATAGACAGGG TACCGGTTCC CTAAGTGAGA GCTCCATCCC CTCTATTTCC 
GACACCAGCA CCCCAAGACG AAGCCGTCGC CAGCTCCCAC CTGTGCCACC AAAGCCTCGT 
CCCCTCCTCT CCTACAGCTC CCTGATGAGA CACACTGGCG GCATCTCTCC ACCTCCTGAT 
GGAAGTGAGG GTGGATCCCC ACTGGCCTCT CAAGCTCTGG AGAGCAACAG CGCTTGCCTG 
ACCGAGTCTT CCAACTCCTT GCACCCCCAG CAGGGCCAGC ATCCTTCCCC ACAGCACTAC 
ATCTCCGAGC CCTATCTGGC CCTCCATGAA GACTCCCACG CCTCAGACTG TGGCGAGGAG 
GAGACACTCA CCTTTGAGGC AGCCGTAGCC ACTAGCTTGG GCCGGTCCAA TACCATTGGC 
TCTGCGCCAC CCCTACGGCA CAGCTGGCAG ATGCCTAATG GGCACTATCG GCGGCGGAGG 
CGTGGGGGGC CTGGGCCTGG CATGATGTGT GGAGCTGTCA GTGACCTCCT GAGTGACACG 
GAAGAAGACG ATAAGTGCTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cacna1e ( XM_011247916.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011246218.1 Mus musculus calcium channel, voltage-dependent, R type, alpha 1E subunit (Cacna1e), transcript variant X17, mRNA.

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CloneID	OMu67267
Clone ID Related Accession (Same CDS sequence)	XM_011247916.3 , XM_011247916.2 , XM_017312848.1
Accession Version	XM_011247916.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6963bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	voltage-dependent R-type calcium channel subunit alpha-1E isoform X14
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_011247916.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTCGCT TCGGGGAGGC GGTGGTCGTT GGCAGGCCAG GCTCAGGCGA TGGAGACTCG 
GACCAGAGCA GGAACCGACA AGGAACCCCC GTCCCGGCCT CGGGGCCGGC GGCCGCCTAC 
AAGCAGTCAA AAGCGCAGAG GGCGCGGACT ATGGCTTTGT ACAACCCCAT ACCAGTCCGG 
CAGAACTGTT TCACGGTCAA CAGATCCCTG TTCATCTTCG GAGAAGATAA CATTGTCAGG 
AAATACGCCA AGAAGCTCAT CGATTGGCCG CCATTTGAGT ACATGATCCT GGCCACCATC 
ATTGCCAACT GCATCGTCTT GGCCCTGGAG CAGCATCTTC CTGAGGATGA CAAGACCCCA 
ATGTCTCGAA GACTGGAGAA GACAGAACCA TATTTCATTG GGATCTTCTG CTTTGAAGCT 
GGGATCAAAA TTGTGGCTCT AGGGTTCATC TTCCATAAGG GTTCATACCT CCGTAATGGC 
TGGAATGTCA TGGACTTCAT CGTGGTCCTT AGTGGCATCC TGGCCACTGC GGGAACCCAC 
TTCAACACCC ACGTGGACCT GCGGACCCTC CGGGCTGTGC GTGTCCTGAG GCCTTTAAAG 
CTGGTATCAG GAATACCTAG CCTGCAGATC GTGCTGAAGT CTATCATGAA GGCCATGGTG 
CCTCTTCTGC AGATTGGCCT TCTGCTCTTC TTTGCCATCC TCATGTTTGC TATCATTGGC 
TTGGAGTTCT ACAGTGGCAA GTTGCATCGA GCATGCTTCA TGAACAATTC AGGTATTCTA 
GAGGGGTTTG ATCCTCCTCA CCCGTGTGGT GTGCAAGGCT GCCCGGCTGG TTATGAATGT 
AAGGACTGGA TCGGCCCCAA TGACGGGATC ACCCAGTTTG ACAACATCCT TTTTGCCGTG 
CTGACTGTCT TCCAATGCAT CACTATGGAA GGGTGGACCA CTGTGCTGTA CAATACCAAT 
GATGCCTTAG GAGCCACCTG GAACTGGCTG TACTTCATCC CTCTCATCAT CATCGGATCC 
TTCTTTGTTC TCAACCTCGT CCTGGGAGTG CTTTCCGGGG AATTTGCCAA AGAAAGAGAG 
AGAGTGGAGA ACCGAAGAGC GTTCATGAAG CTCCGGCGCC AGCAACAGAT TGAGCGAGAA 
TTGAACGGCT ACCGTGCCTG GATAGACAAA GCAGAGGAAG TCATGCTTGC TGAAGAAAAT 
AAAAACTCGG GAACATCAGC CTTGGAAGTG CTTCGAAGGG CAACCATCAA AAGGAGCCGG 
ACGGAGGCCA TGACCCGAGA CTCCAGCGAT GAGCACTGCG TTGATATCTC TTCAGTGGGC 
ACACCTCTTG CCAGAGCCAG TATCAAGAGC ACAAAAGTAG ATGGGGCCTC CTATTTCCGG 
CACAAGGAAC GGCTTCTGCG CATCTCTATC CGCCACATGG TCAAGTCCCA AGTCTTTTAC 
TGGATCGTCC TGAGTGTTGT GGCTCTTAAT ACTGCCTGTG TGGCCATTGT TCATCACAAC 
CAGCCACAGT GGCTCACTCA CCTCCTCTAC TATGCAGAGT TTTTGTTTCT GGGACTCTTC 
CTGTTGGAGA TGTCCCTGAA GATGTATGGC ATGGGGCCAC GCCTCTATTT TCACTCATCC 
TTCAACTGCT TTGATTTTGG GGTCACAGTG GGCAGCATCT TTGAGGTTGT CTGGGCAATC 
TTCAGACCTG GCACATCTTT TGGAATCAGT GTCTTACGAG CTCTCCGGCT CCTAAGGATA 
TTTAAAATAA CCAAGTATTG GGCATCCCTC CGGAATCTGG TTGTCTCCTT GATGAGCTCC 
ATGAAGTCTA TCATCAGCTT ACTCTTCCTT CTCTTCCTCT TCATCGTTGT CTTTGCTCTC 
CTGGGAATGC AGCTCTTTGG AGGCAGGTTT AACTTTAATG ATGGGACTCC TTCAGCTAAT 
TTTGACACCT TCCCTGCAGC TATCATGACA GTGTTCCAGA TCCTGACAGG GGAAGACTGG 
AATGAGGTGA TGTACAATGG CATTCGCTCC CAGGGTGGGG TCAGCTCAGG CATGTGGTCT 
GCCATCTACT TCATTGTGCT CACCTTGTTT GGAAACTACA CGCTGCTAAA TGTATTCTTG 
GCCATCGCTG TGGACAATCT GGCCAATGCC CAGGAACTGA CCAAGGATGA GCAGGAGGAA 
GAAGAAGCCT TCAACCAGAA ACATGCACTG CAGAAGGCCA AGGAGGTCAG CCCGATGTCT 
GCTCCCAACA TGCCTTCCAT TGAAAGAGAC AGAAGGAGAA GACACCACAT GTCGATGTGG 
GAGCCACGCA GCAGCCACCT GAGGGAGCGG AGGCGCCGGC ACCACATGTC TGTGTGGGAA 
CAACGCACCA GCCAGCTGAG GAGGCACATG CAAATGTCTA GCCAAGAAGC CCTCAACAAA 
GAGGAGGCCC CGCCAATGAA CCCCCTCAAT CCACTCAACC CACTGAGTCC TCTCAACCCC 
CTCAATGCTC ACCCCAGCCT TTATCGGAGG CCCAGGCCCA TTGAGGGCCT AGCCCTAGGT 
CTGGGTCTTG AAAAGTGTGA AGAAGAACGC ATCAGTCGTG GGGGCTCCCT CAAGGGGGAC 
ATAGGGGGCC TGACTAGCGC CCTGGACAAC CAGAGGAGCC CCTTGTCCTT GGGCAAACGG 
GAGCCACCGT GGCTGCCTAG ATCCTGTCAT GGAAACTGTG ACCCAATCCA GCAGGAGGCT 
GGGGGAGGAG AGACTGTGGT GACTTTTGAG GACCGGGCCA GGCACAGGCA GAGTCAGAGG 
CGAAGCCGGC ATCGCCGAGT CAGGACTGAG GGCAAGGACT CTGCCTCTGC ATCCCGAAGC 
AGGTCTGCCA GCCAGGAGCG GAGTCTGGAT GAAGGGGTGT CCGTTGAGGG GGAGAAGGAA 
CATGAGCCTC ACAGCAGCCA CAGGAGCAAA GAGCCGACCA TCCACGAAGA GGAGAGAACC 
CAGGACTTAA GGAGGACCAA CAGTCTGATG GTCCCCAGGG GCTCTGGACT GGTAGGGGCT 
CTTGATGAAG CAGAAACACC TCTAGTCCAG CCCCAACCTG AGCTGGAAGT GGGGAAGGAT 
GCAGCTCTGA CAGAGCAGGA GGCTGAAGGC AGCAGTGAAC AAGCCCTTCT TGGGGACGTA 
CAGCTGGATG TGGGCCGGGG CATTAGCCAG AGTGAACCGG ATCTCTCCTG CATGACAGCC 
AACATGGACA AGGCTACCAC GGAGAGCACC AGTGTCACGG TCGCCATCCC TGATGTGGAC 
CCCTTGGTGG ACTCGACAGT GGTGAACATT AGCAACAAGA CGGATGGAGA AGCCAGTCCC 
TTGAAGGAGG CAGAGACTAA AGAGGAAGAA GAAGAGGTAG AGAAGAAGAA GAAGCAGAAG 
AAGGAGAAGC GAGAGACTGG AAAAGCCATG GTGCCCCATA GCTCTATGTT CATCTTCAGC 
ACCACTAACC CGATCCGCAG GGCCTGCCAC TACATTGTGA ACCTACGCTA CTTTGAAATG 
TGCATTCTCT TGGTGATTGC GGCCAGCAGC ATTGCCCTGG CAGCCGAGGA CCCTGTACTA 
ACTAACTCGG AGCGTAACAA AGTCCTGAGA TATTTCGACT ATGTTTTCAC AGGAGTGTTT 
ACCTTTGAGA TGGTTATAAA GATGATAGAC CAAGGCTTGA TCCTGCAGGA TGGATCATAC 
TTCAGAGACC TCTGGAACAT CCTGGACTTT GTAGTAGTGG TTGGTGCATT GGTAGCATTT 
GCTCTGGCGA ACGCTTTGGG AACCAACAAG GGGAGGGATA TCAAGACTAT CAAGTCTCTG 
AGGGTACTTC GAGTTCTGCG GCCACTGAAA ACCATCAAGC GCTTGCCCAA GCTGAAGGCT 
GTGTTTGACT GCGTGGTGAC CTCCTTGAAG AATGTCTTCA ACATACTCAT TGTCTACAAG 
CTCTTCATGT TCATCTTTGC CGTCATCGCT GTTCAGCTAT TCAAAGGAAA GTTCTTTTAT 
TGCACTGACA GTTCCAAGGA CACAGAGAAG GAGTGCATAG GCAACTATGT AGACCACGAG 
AAAAACAAGA TGGAGGTGAA GGGCCGGGAA TGGAAGCGTC ATGAATTCCA CTATGACAAC 
ATCATCTGGG CCCTGCTTAC CCTCTTCACA GTCTCTACAG GGGAAGGATG GCCTCAGGTT 
CTGCAGCACT CTGTAGATGT GACAGAGGAG GACAGAGGCC CAAGTCGCAG CAACCGCATG 
GAGATGTCCA TCTTTTATGT GGTCTACTTT GTGGTCTTCC CTTTCTTCTT TGTCAATATC 
TTCGTGGCTC TCATTATCAT CACCTTCCAG GAGCAAGGGG ACAAGATGAT GGAGGAGTGT 
AGCCTGGAGA AGAATGAGCG GGCATGCATC GACTTCGCCA TTAGTGCCAA ACCTCTCACC 
CGCTACATGC CACAGAACAG GCACACCTTC CAGTACCGTG TGTGGCACTT TGTAGTCTCC 
CCATCCTTTG AGTATACCAT CATGGCCATG ATTGCCTTGA ACACTGTCGT GCTGATGATG 
AAGTACTACT CTGCTCCTTG CACCTATGAG CTCGCCTTAA AGTACCTGAA CATCGCCTTC 
ACTATGGTGT TTTCCCTGGA GTGTGTCCTA AAGGTCATTG CTTTCGGCTT CCTGAACTAT 
TTCCGAGATA CCTGGAACAT CTTTGACTTC ATCACGGTGA TCGGCAGTAT CACAGAAATT 
ATCCTGACAG ATAGCAAGCT GGTGAACACC AGTGGCTTCA ATATGAGCTT TCTGAAGCTC 
TTCCGAGCTG CACGGCTCAT AAAGCTTCTG AGACAGGGCT ACACCATCCG CATCTTACTG 
TGGACCTTCG TGCAGTCCTT TAAGGCTCTT CCCTACGTCT GCCTTCTCAT TGCCATGCTT 
TTCTTCATCT ACGCCATCAT TGGGATGCAG GTGTTTGGAA ACATAAAGTT AGATGAGGAG 
AGTCACATCA ACCGGCACAA CAACTTCCGG AGTTTCTTTG GATCCCTCAT GCTGCTTTTC 
AGGAGTGCCA CGGGGGAGGC TTGGCAGGAG ATTATGCTGT CCTGTCTCGG TGAGAAGGGT 
TGTGAGCCTG ACACCACTGC ACCCTCAGGG CAGAATGAGA GTGAGCGCTG TGGCACTGAC 
CTGGCCTACG TTTATTTTGT CTCCTTCATC TTCTTCTGCT CCTTCCTGAT GCTCAACCTG 
TTCGTGGCTG TCATCATGGA CAACTTTGAG TACCTGACTC GAGATTCCTC CATCCTGGGG 
CCTCACCACT TAGATGAGTT TGTCCGTGTC TGGGCAGAAT ATGACAGAGC AGCATGTGGC 
CGCATCCATT ACACTGAGAT GTATGAAATG CTGACTCTCA TGTCACCACC GCTAGGCCTC 
GGCAAGAGAT GTCCCTCCAA AGTGGCCTAT AAGAGGCTGG TCCTGATGAA CATGCCAGTG 
GCTGAGGACA TGACAGTCCA CTTCACCTCC ACACTTATGG CTCTGATCCG GACAGCTCTG 
GACATTAAAA TCGCCAAAGG TGGTGCAGAC AGACAGCAGC TAGACTCGGA GCTGCAAAAA 
GAGACCCTGG CCATTTGGCC TCACCTGTCC CAAAAGATGT TGGATCTGCT TGTGCCCATG 
CCCAAAGCCT CTGACCTGAC TGTGGGCAAG ATCTATGCAG CAATGATGAT CATGGACTAC 
TATAAACAGA GTAAGGTAAA GAAACAGAGA CAGCAGCTGG AAGAACAGAA AAATGCACCC 
ATGTTCCAGC GCATGGAGCC CTCATCACTG CCTCAGGAGA TCATTGCTAA TGCCAAAGCC 
CTGCCTTATC TCCAGCAGGA CCCTGTTTCT GGCCTGAGTG GTCGGAGTGG ATACCCTTCA 
ATGAGTCCAC TCTCCCCTCA AGAAATATTC CAGTTGGCTT GTATGGACCC AGCGGATGAT 
GGACAATTCC AGGAACAACA ATCTCTGGTG AATGCACAAG AGCCAGAGGT TAGTGAATTA 
AAAAGTGTGC AGTCCTCTAA CCATGGCATC TACCTTCCTC CGGACACCCA GGAGCATGCG 
GGATCTGGGA GGGCATCCTC TATGCCACGT CTGACTATGG ATCCCCAGGT GGTAACAGAC 
CCTAGCTCTA TGCGACGTTC ATTTTCTACA ATTCGGGATA AGCGTTCAAA TTCCTCCTGG 
TTGGAGGAGT TCTCCATGGA ACGCAGCAGT GAAAACACTT ACAAGTCGCG GCGCAGAAGT 
TACCATTCCT CCCTGAGGCT GTCAGCACAC CGCCTGAATT CTGACTCTGG TCACAAGTCT 
GACACTCACC GATCAGGAGG CAGGGAGAGA GGCCGATCCA AAGAACGAAA GCACCTCCTC 
TCTCCAGATG TCTCCCGCTG CAATTCTGAA GAGCGAGGGA CCCAGGCCGA CTGGGAGTCC 
CCAGAACGTC GCCAGTCCAG GTCACCCAGT GAAGGAAGGT CACAGACCCC CAATAGACAG 
GGTACCGGTT CCCTAAGTGA GAGCTCCATC CCCTCTATTT CCGACACCAG CACCCCAAGA 
CGAAGCCGTC GCCAGCTCCC ACCTGTGCCA CCAAAGCCTC GTCCCCTCCT CTCCTACAGC 
TCCCTGATGA GACACACTGG CGGCATCTCT CCACCTCCTG ATGGAAGTGA GGGTGGATCC 
CCACTGGCCT CTCAAGCTCT GGAGAGCAAC AGCGCTTGCC TGACCGAGTC TTCCAACTCC 
TTGCACCCCC AGCAGGGCCA GCATCCTTCC CCACAGCACT ACATCTCCGA GCCCTATCTG 
GCCCTCCATG AAGACTCCCA CGCCTCAGAC TGTGGCGAGG AGGAGACACT CACCTTTGAG 
GCAGCCGTAG CCACTAGCTT GGGCCGGTCC AATACCATTG GCTCTGCGCC ACCCCTACGG 
CACAGCTGGC AGATGCCTAA TGGGCACTAT CGGCGGCGGA GGCGTGGGGG GCCTGGGCCT 
GGCATGATGT GTGGAGCTGT CAGTGACCTC CTGAGTGACA CGGAAGAAGA CGATAAGTGC 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011246218.1
CDS	775..7737
Translation	MARFGEAVVVGRPGSGDGDSDQSRNRQGTPVPASGPAAAYKQSKAQRARTMALYNPIPVRQNCFTVNRSLFIFGEDNIVRKYAKKLIDWPPFEYMILATIIANCIVLALEQHLPEDDKTPMSRRLEKTEPYFIGIFCFEAGIKIVALGFIFHKGSYLRNGWNVMDFIVVLSGILATAGTHFNTHVDLRTLRAVRVLRPLKLVSGIPSLQIVLKSIMKAMVPLLQIGLLLFFAILMFAIIGLEFYSGKLHRACFMNNSGILEGFDPPHPCGVQGCPAGYECKDWIGPNDGITQFDNILFAVLTVFQCITMEGWTTVLYNTNDALGATWNWLYFIPLIIIGSFFVLNLVLGVLSGEFAKERERVENRRAFMKLRRQQQIERELNGYRAWIDKAEEVMLAEENKNSGTSALEVLRRATIKRSRTEAMTRDSSDEHCVDISSVGTPLARASIKSTKVDGASYFRHKERLLRISIRHMVKSQVFYWIVLSVVALNTACVAIVHHNQPQWLTHLLYYAEFLFLGLFLLEMSLKMYGMGPRLYFHSSFNCFDFGVTVGSIFEVVWAIFRPGTSFGISVLRALRLLRIFKITKYWASLRNLVVSLMSSMKSIISLLFLLFLFIVVFALLGMQLFGGRFNFNDGTPSANFDTFPAAIMTVFQILTGEDWNEVMYNGIRSQGGVSSGMWSAIYFIVLTLFGNYTLLNVFLAIAVDNLANAQELTKDEQEEEEAFNQKHALQKAKEVSPMSAPNMPSIERDRRRRHHMSMWEPRSSHLRERRRRHHMSVWEQRTSQLRRHMQMSSQEALNKEEAPPMNPLNPLNPLSPLNPLNAHPSLYRRPRPIEGLALGLGLEKCEEERISRGGSLKGDIGGLTSALDNQRSPLSLGKREPPWLPRSCHGNCDPIQQEAGGGETVVTFEDRARHRQSQRRSRHRRVRTEGKDSASASRSRSASQERSLDEGVSVEGEKEHEPHSSHRSKEPTIHEEERTQDLRRTNSLMVPRGSGLVGALDEAETPLVQPQPELEVGKDAALTEQEAEGSSEQALLGDVQLDVGRGISQSEPDLSCMTANMDKATTESTSVTVAIPDVDPLVDSTVVNISNKTDGEASPLKEAETKEEEEEVEKKKKQKKEKRETGKAMVPHSSMFIFSTTNPIRRACHYIVNLRYFEMCILLVIAASSIALAAEDPVLTNSERNKVLRYFDYVFTGVFTFEMVIKMIDQGLILQDGSYFRDLWNILDFVVVVGALVAFALANALGTNKGRDIKTIKSLRVLRVLRPLKTIKRLPKLKAVFDCVVTSLKNVFNILIVYKLFMFIFAVIAVQLFKGKFFYCTDSSKDTEKECIGNYVDHEKNKMEVKGREWKRHEFHYDNIIWALLTLFTVSTGEGWPQVLQHSVDVTEEDRGPSRSNRMEMSIFYVVYFVVFPFFFVNIFVALIIITFQEQGDKMMEECSLEKNERACIDFAISAKPLTRYMPQNRHTFQYRVWHFVVSPSFEYTIMAMIALNTVVLMMKYYSAPCTYELALKYLNIAFTMVFSLECVLKVIAFGFLNYFRDTWNIFDFITVIGSITEIILTDSKLVNTSGFNMSFLKLFRAARLIKLLRQGYTIRILLWTFVQSFKALPYVCLLIAMLFFIYAIIGMQVFGNIKLDEESHINRHNNFRSFFGSLMLLFRSATGEAWQEIMLSCLGEKGCEPDTTAPSGQNESERCGTDLAYVYFVSFIFFCSFLMLNLFVAVIMDNFEYLTRDSSILGPHHLDEFVRVWAEYDRAACGRIHYTEMYEMLTLMSPPLGLGKRCPSKVAYKRLVLMNMPVAEDMTVHFTSTLMALIRTALDIKIAKGGADRQQLDSELQKETLAIWPHLSQKMLDLLVPMPKASDLTVGKIYAAMMIMDYYKQSKVKKQRQQLEEQKNAPMFQRMEPSSLPQEIIANAKALPYLQQDPVSGLSGRSGYPSMSPLSPQEIFQLACMDPADDGQFQEQQSLVNAQEPEVSELKSVQSSNHGIYLPPDTQEHAGSGRASSMPRLTMDPQVVTDPSSMRRSFSTIRDKRSNSSWLEEFSMERSSENTYKSRRRSYHSSLRLSAHRLNSDSGHKSDTHRSGGRERGRSKERKHLLSPDVSRCNSEERGTQADWESPERRQSRSPSEGRSQTPNRQGTGSLSESSIPSISDTSTPRRSRRQLPPVPPKPRPLLSYSSLMRHTGGISPPPDGSEGGSPLASQALESNSACLTESSNSLHPQQGQHPSPQHYISEPYLALHEDSHASDCGEEETLTFEAAVATSLGRSNTIGSAPPLRHSWQMPNGHYRRRRRGGPGPGMMCGAVSDLLSDTEEDDKC

Target ORF information:

RefSeq Version	XM_011247916.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus calcium channel, voltage-dependent, R type, alpha 1E subunit (Cacna1e), transcript variant X17, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011247916.3

ORF Insert Sequence:

ATGGCTCGCT TCGGGGAGGC GGTGGTCGTT GGCAGGCCAG GCTCAGGCGA TGGAGACTCG 
GACCAGAGCA GGAACCGACA AGGAACCCCC GTCCCGGCCT CGGGGCCGGC GGCCGCCTAC 
AAGCAGTCAA AAGCGCAGAG GGCGCGGACT ATGGCTTTGT ACAACCCCAT ACCAGTCCGG 
CAGAACTGTT TCACGGTCAA CAGATCCCTG TTCATCTTCG GAGAAGATAA CATTGTCAGG 
AAATACGCCA AGAAGCTCAT CGATTGGCCG CCATTTGAGT ACATGATCCT GGCCACCATC 
ATTGCCAACT GCATCGTCTT GGCCCTGGAG CAGCATCTTC CTGAGGATGA CAAGACCCCA 
ATGTCTCGAA GACTGGAGAA GACAGAACCA TATTTCATTG GGATCTTCTG CTTTGAAGCT 
GGGATCAAAA TTGTGGCTCT AGGGTTCATC TTCCATAAGG GTTCATACCT CCGTAATGGC 
TGGAATGTCA TGGACTTCAT CGTGGTCCTT AGTGGCATCC TGGCCACTGC GGGAACCCAC 
TTCAACACCC ACGTGGACCT GCGGACCCTC CGGGCTGTGC GTGTCCTGAG GCCTTTAAAG 
CTGGTATCAG GAATACCTAG CCTGCAGATC GTGCTGAAGT CTATCATGAA GGCCATGGTG 
CCTCTTCTGC AGATTGGCCT TCTGCTCTTC TTTGCCATCC TCATGTTTGC TATCATTGGC 
TTGGAGTTCT ACAGTGGCAA GTTGCATCGA GCATGCTTCA TGAACAATTC AGGTATTCTA 
GAGGGGTTTG ATCCTCCTCA CCCGTGTGGT GTGCAAGGCT GCCCGGCTGG TTATGAATGT 
AAGGACTGGA TCGGCCCCAA TGACGGGATC ACCCAGTTTG ACAACATCCT TTTTGCCGTG 
CTGACTGTCT TCCAATGCAT CACTATGGAA GGGTGGACCA CTGTGCTGTA CAATACCAAT 
GATGCCTTAG GAGCCACCTG GAACTGGCTG TACTTCATCC CTCTCATCAT CATCGGATCC 
TTCTTTGTTC TCAACCTCGT CCTGGGAGTG CTTTCCGGGG AATTTGCCAA AGAAAGAGAG 
AGAGTGGAGA ACCGAAGAGC GTTCATGAAG CTCCGGCGCC AGCAACAGAT TGAGCGAGAA 
TTGAACGGCT ACCGTGCCTG GATAGACAAA GCAGAGGAAG TCATGCTTGC TGAAGAAAAT 
AAAAACTCGG GAACATCAGC CTTGGAAGTG CTTCGAAGGG CAACCATCAA AAGGAGCCGG 
ACGGAGGCCA TGACCCGAGA CTCCAGCGAT GAGCACTGCG TTGATATCTC TTCAGTGGGC 
ACACCTCTTG CCAGAGCCAG TATCAAGAGC ACAAAAGTAG ATGGGGCCTC CTATTTCCGG 
CACAAGGAAC GGCTTCTGCG CATCTCTATC CGCCACATGG TCAAGTCCCA AGTCTTTTAC 
TGGATCGTCC TGAGTGTTGT GGCTCTTAAT ACTGCCTGTG TGGCCATTGT TCATCACAAC 
CAGCCACAGT GGCTCACTCA CCTCCTCTAC TATGCAGAGT TTTTGTTTCT GGGACTCTTC 
CTGTTGGAGA TGTCCCTGAA GATGTATGGC ATGGGGCCAC GCCTCTATTT TCACTCATCC 
TTCAACTGCT TTGATTTTGG GGTCACAGTG GGCAGCATCT TTGAGGTTGT CTGGGCAATC 
TTCAGACCTG GCACATCTTT TGGAATCAGT GTCTTACGAG CTCTCCGGCT CCTAAGGATA 
TTTAAAATAA CCAAGTATTG GGCATCCCTC CGGAATCTGG TTGTCTCCTT GATGAGCTCC 
ATGAAGTCTA TCATCAGCTT ACTCTTCCTT CTCTTCCTCT TCATCGTTGT CTTTGCTCTC 
CTGGGAATGC AGCTCTTTGG AGGCAGGTTT AACTTTAATG ATGGGACTCC TTCAGCTAAT 
TTTGACACCT TCCCTGCAGC TATCATGACA GTGTTCCAGA TCCTGACAGG GGAAGACTGG 
AATGAGGTGA TGTACAATGG CATTCGCTCC CAGGGTGGGG TCAGCTCAGG CATGTGGTCT 
GCCATCTACT TCATTGTGCT CACCTTGTTT GGAAACTACA CGCTGCTAAA TGTATTCTTG 
GCCATCGCTG TGGACAATCT GGCCAATGCC CAGGAACTGA CCAAGGATGA GCAGGAGGAA 
GAAGAAGCCT TCAACCAGAA ACATGCACTG CAGAAGGCCA AGGAGGTCAG CCCGATGTCT 
GCTCCCAACA TGCCTTCCAT TGAAAGAGAC AGAAGGAGAA GACACCACAT GTCGATGTGG 
GAGCCACGCA GCAGCCACCT GAGGGAGCGG AGGCGCCGGC ACCACATGTC TGTGTGGGAA 
CAACGCACCA GCCAGCTGAG GAGGCACATG CAAATGTCTA GCCAAGAAGC CCTCAACAAA 
GAGGAGGCCC CGCCAATGAA CCCCCTCAAT CCACTCAACC CACTGAGTCC TCTCAACCCC 
CTCAATGCTC ACCCCAGCCT TTATCGGAGG CCCAGGCCCA TTGAGGGCCT AGCCCTAGGT 
CTGGGTCTTG AAAAGTGTGA AGAAGAACGC ATCAGTCGTG GGGGCTCCCT CAAGGGGGAC 
ATAGGGGGCC TGACTAGCGC CCTGGACAAC CAGAGGAGCC CCTTGTCCTT GGGCAAACGG 
GAGCCACCGT GGCTGCCTAG ATCCTGTCAT GGAAACTGTG ACCCAATCCA GCAGGAGGCT 
GGGGGAGGAG AGACTGTGGT GACTTTTGAG GACCGGGCCA GGCACAGGCA GAGTCAGAGG 
CGAAGCCGGC ATCGCCGAGT CAGGACTGAG GGCAAGGACT CTGCCTCTGC ATCCCGAAGC 
AGGTCTGCCA GCCAGGAGCG GAGTCTGGAT GAAGGGGTGT CCGTTGAGGG GGAGAAGGAA 
CATGAGCCTC ACAGCAGCCA CAGGAGCAAA GAGCCGACCA TCCACGAAGA GGAGAGAACC 
CAGGACTTAA GGAGGACCAA CAGTCTGATG GTCCCCAGGG GCTCTGGACT GGTAGGGGCT 
CTTGATGAAG CAGAAACACC TCTAGTCCAG CCCCAACCTG AGCTGGAAGT GGGGAAGGAT 
GCAGCTCTGA CAGAGCAGGA GGCTGAAGGC AGCAGTGAAC AAGCCCTTCT TGGGGACGTA 
CAGCTGGATG TGGGCCGGGG CATTAGCCAG AGTGAACCGG ATCTCTCCTG CATGACAGCC 
AACATGGACA AGGCTACCAC GGAGAGCACC AGTGTCACGG TCGCCATCCC TGATGTGGAC 
CCCTTGGTGG ACTCGACAGT GGTGAACATT AGCAACAAGA CGGATGGAGA AGCCAGTCCC 
TTGAAGGAGG CAGAGACTAA AGAGGAAGAA GAAGAGGTAG AGAAGAAGAA GAAGCAGAAG 
AAGGAGAAGC GAGAGACTGG AAAAGCCATG GTGCCCCATA GCTCTATGTT CATCTTCAGC 
ACCACTAACC CGATCCGCAG GGCCTGCCAC TACATTGTGA ACCTACGCTA CTTTGAAATG 
TGCATTCTCT TGGTGATTGC GGCCAGCAGC ATTGCCCTGG CAGCCGAGGA CCCTGTACTA 
ACTAACTCGG AGCGTAACAA AGTCCTGAGA TATTTCGACT ATGTTTTCAC AGGAGTGTTT 
ACCTTTGAGA TGGTTATAAA GATGATAGAC CAAGGCTTGA TCCTGCAGGA TGGATCATAC 
TTCAGAGACC TCTGGAACAT CCTGGACTTT GTAGTAGTGG TTGGTGCATT GGTAGCATTT 
GCTCTGGCGA ACGCTTTGGG AACCAACAAG GGGAGGGATA TCAAGACTAT CAAGTCTCTG 
AGGGTACTTC GAGTTCTGCG GCCACTGAAA ACCATCAAGC GCTTGCCCAA GCTGAAGGCT 
GTGTTTGACT GCGTGGTGAC CTCCTTGAAG AATGTCTTCA ACATACTCAT TGTCTACAAG 
CTCTTCATGT TCATCTTTGC CGTCATCGCT GTTCAGCTAT TCAAAGGAAA GTTCTTTTAT 
TGCACTGACA GTTCCAAGGA CACAGAGAAG GAGTGCATAG GCAACTATGT AGACCACGAG 
AAAAACAAGA TGGAGGTGAA GGGCCGGGAA TGGAAGCGTC ATGAATTCCA CTATGACAAC 
ATCATCTGGG CCCTGCTTAC CCTCTTCACA GTCTCTACAG GGGAAGGATG GCCTCAGGTT 
CTGCAGCACT CTGTAGATGT GACAGAGGAG GACAGAGGCC CAAGTCGCAG CAACCGCATG 
GAGATGTCCA TCTTTTATGT GGTCTACTTT GTGGTCTTCC CTTTCTTCTT TGTCAATATC 
TTCGTGGCTC TCATTATCAT CACCTTCCAG GAGCAAGGGG ACAAGATGAT GGAGGAGTGT 
AGCCTGGAGA AGAATGAGCG GGCATGCATC GACTTCGCCA TTAGTGCCAA ACCTCTCACC 
CGCTACATGC CACAGAACAG GCACACCTTC CAGTACCGTG TGTGGCACTT TGTAGTCTCC 
CCATCCTTTG AGTATACCAT CATGGCCATG ATTGCCTTGA ACACTGTCGT GCTGATGATG 
AAGTACTACT CTGCTCCTTG CACCTATGAG CTCGCCTTAA AGTACCTGAA CATCGCCTTC 
ACTATGGTGT TTTCCCTGGA GTGTGTCCTA AAGGTCATTG CTTTCGGCTT CCTGAACTAT 
TTCCGAGATA CCTGGAACAT CTTTGACTTC ATCACGGTGA TCGGCAGTAT CACAGAAATT 
ATCCTGACAG ATAGCAAGCT GGTGAACACC AGTGGCTTCA ATATGAGCTT TCTGAAGCTC 
TTCCGAGCTG CACGGCTCAT AAAGCTTCTG AGACAGGGCT ACACCATCCG CATCTTACTG 
TGGACCTTCG TGCAGTCCTT TAAGGCTCTT CCCTACGTCT GCCTTCTCAT TGCCATGCTT 
TTCTTCATCT ACGCCATCAT TGGGATGCAG GTGTTTGGAA ACATAAAGTT AGATGAGGAG 
AGTCACATCA ACCGGCACAA CAACTTCCGG AGTTTCTTTG GATCCCTCAT GCTGCTTTTC 
AGGAGTGCCA CGGGGGAGGC TTGGCAGGAG ATTATGCTGT CCTGTCTCGG TGAGAAGGGT 
TGTGAGCCTG ACACCACTGC ACCCTCAGGG CAGAATGAGA GTGAGCGCTG TGGCACTGAC 
CTGGCCTACG TTTATTTTGT CTCCTTCATC TTCTTCTGCT CCTTCCTGAT GCTCAACCTG 
TTCGTGGCTG TCATCATGGA CAACTTTGAG TACCTGACTC GAGATTCCTC CATCCTGGGG 
CCTCACCACT TAGATGAGTT TGTCCGTGTC TGGGCAGAAT ATGACAGAGC AGCATGTGGC 
CGCATCCATT ACACTGAGAT GTATGAAATG CTGACTCTCA TGTCACCACC GCTAGGCCTC 
GGCAAGAGAT GTCCCTCCAA AGTGGCCTAT AAGAGGCTGG TCCTGATGAA CATGCCAGTG 
GCTGAGGACA TGACAGTCCA CTTCACCTCC ACACTTATGG CTCTGATCCG GACAGCTCTG 
GACATTAAAA TCGCCAAAGG TGGTGCAGAC AGACAGCAGC TAGACTCGGA GCTGCAAAAA 
GAGACCCTGG CCATTTGGCC TCACCTGTCC CAAAAGATGT TGGATCTGCT TGTGCCCATG 
CCCAAAGCCT CTGACCTGAC TGTGGGCAAG ATCTATGCAG CAATGATGAT CATGGACTAC 
TATAAACAGA GTAAGGTAAA GAAACAGAGA CAGCAGCTGG AAGAACAGAA AAATGCACCC 
ATGTTCCAGC GCATGGAGCC CTCATCACTG CCTCAGGAGA TCATTGCTAA TGCCAAAGCC 
CTGCCTTATC TCCAGCAGGA CCCTGTTTCT GGCCTGAGTG GTCGGAGTGG ATACCCTTCA 
ATGAGTCCAC TCTCCCCTCA AGAAATATTC CAGTTGGCTT GTATGGACCC AGCGGATGAT 
GGACAATTCC AGGAACAACA ATCTCTGGTG AATGCACAAG AGCCAGAGGT TAGTGAATTA 
AAAAGTGTGC AGTCCTCTAA CCATGGCATC TACCTTCCTC CGGACACCCA GGAGCATGCG 
GGATCTGGGA GGGCATCCTC TATGCCACGT CTGACTATGG ATCCCCAGGT GGTAACAGAC 
CCTAGCTCTA TGCGACGTTC ATTTTCTACA ATTCGGGATA AGCGTTCAAA TTCCTCCTGG 
TTGGAGGAGT TCTCCATGGA ACGCAGCAGT GAAAACACTT ACAAGTCGCG GCGCAGAAGT 
TACCATTCCT CCCTGAGGCT GTCAGCACAC CGCCTGAATT CTGACTCTGG TCACAAGTCT 
GACACTCACC GATCAGGAGG CAGGGAGAGA GGCCGATCCA AAGAACGAAA GCACCTCCTC 
TCTCCAGATG TCTCCCGCTG CAATTCTGAA GAGCGAGGGA CCCAGGCCGA CTGGGAGTCC 
CCAGAACGTC GCCAGTCCAG GTCACCCAGT GAAGGAAGGT CACAGACCCC CAATAGACAG 
GGTACCGGTT CCCTAAGTGA GAGCTCCATC CCCTCTATTT CCGACACCAG CACCCCAAGA 
CGAAGCCGTC GCCAGCTCCC ACCTGTGCCA CCAAAGCCTC GTCCCCTCCT CTCCTACAGC 
TCCCTGATGA GACACACTGG CGGCATCTCT CCACCTCCTG ATGGAAGTGA GGGTGGATCC 
CCACTGGCCT CTCAAGCTCT GGAGAGCAAC AGCGCTTGCC TGACCGAGTC TTCCAACTCC 
TTGCACCCCC AGCAGGGCCA GCATCCTTCC CCACAGCACT ACATCTCCGA GCCCTATCTG 
GCCCTCCATG AAGACTCCCA CGCCTCAGAC TGTGGCGAGG AGGAGACACT CACCTTTGAG 
GCAGCCGTAG CCACTAGCTT GGGCCGGTCC AATACCATTG GCTCTGCGCC ACCCCTACGG 
CACAGCTGGC AGATGCCTAA TGGGCACTAT CGGCGGCGGA GGCGTGGGGG GCCTGGGCCT 
GGCATGATGT GTGGAGCTGT CAGTGACCTC CTGAGTGACA CGGAAGAAGA CGATAAGTGC 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cacna1e ( XM_017312766.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017168255.1 Mus musculus calcium channel, voltage-dependent, R type, alpha 1E subunit (Cacna1e), transcript variant X14, mRNA.

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CloneID	OMu76948
Clone ID Related Accession (Same CDS sequence)	XM_017312766.1
Accession Version	XM_017312766.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7260bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	voltage-dependent R-type calcium channel subunit alpha-1E isoform X11
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGCCCG AGATGCGTGG ACTGAGCCCA GGCCGAAGGC TGCCCGAGCC CACCCTGTGC 
CCGGCTACAG GAGGGAGCCC TGAGCCCGGG GCTGCTGGCA GGCGCCGCCA CGTCCTGGCT 
CTGCCTGATG AGGAGGCATG GCGGCCGCAG CAGTGCGCCC TGCTCGAGGC CAATGCCTCC 
GACGAGGTGG GCATGTTGCC TGCTTCCCCG CGCTCCGCGG TGGCCGGCTT CGACAACTTC 
TTCCCGGTGC AGGAAGGCGA GGGCCCGGGC TGGGAGGGCA GCACCACGCT GGATGCAGGC 
TCCAGCCCCT TCCTTCCTGT GAGCCCAGAG GTCATGAAGC GGAGGCGAGG GGGGCTCATC 
GAGCAGCGGG ACATCATCAA GGCCCACGAG GCACACAAGA TGCAGAGCAC CCCGCAGGCC 
CGGCGCAAGG AGTGGGAGAT GGCTCGCTTC GGGGAGGCGG TGGTCGTTGG CAGGCCAGGC 
TCAGGCGATG GAGACTCGGA CCAGAGCAGG AACCGACAAG GAACCCCCGT CCCGGCCTCG 
GGGCCGGCGG CCGCCTACAA GCAGTCAAAA GCGCAGAGGG CGCGGACTAT GGCTTTGTAC 
AACCCCATAC CAGTCCGGCA GAACTGTTTC ACGGTCAACA GATCCCTGTT CATCTTCGGA 
GAAGATAACA TTGTCAGGAA ATACGCCAAG AAGCTCATCG ATTGGCCGCC ATTTGAGTAC 
ATGATCCTGG CCACCATCAT TGCCAACTGC ATCGTCTTGG CCCTGGAGCA GCATCTTCCT 
GAGGATGACA AGACCCCAAT GTCTCGAAGA CTGGAGAAGA CAGAACCATA TTTCATTGGG 
ATCTTCTGCT TTGAAGCTGG GATCAAAATT GTGGCTCTAG GGTTCATCTT CCATAAGGGT 
TCATACCTCC GTAATGGCTG GAATGTCATG GACTTCATCG TGGTCCTTAG TGGCATCCTG 
GCCACTGCGG GAACCCACTT CAACACCCAC GTGGACCTGC GGACCCTCCG GGCTGTGCGT 
GTCCTGAGGC CTTTAAAGCT GGTATCAGGA ATACCTAGCC TGCAGATCGT GCTGAAGTCT 
ATCATGAAGG CCATGGTGCC TCTTCTGCAG ATTGGCCTTC TGCTCTTCTT TGCCATCCTC 
ATGTTTGCTA TCATTGGCTT GGAGTTCTAC AGTGGCAAGT TGCATCGAGC ATGCTTCATG 
AACAATTCAG GTATTCTAGA GGGGTTTGAT CCTCCTCACC CGTGTGGTGT GCAAGGCTGC 
CCGGCTGGTT ATGAATGTAA GGACTGGATC GGCCCCAATG ACGGGATCAC CCAGTTTGAC 
AACATCCTTT TTGCCGTGCT GACTGTCTTC CAATGCATCA CTATGGAAGG GTGGACCACT 
GTGCTGTACA ATACCAATGA TGCCTTAGGA GCCACCTGGA ACTGGCTGTA CTTCATCCCT 
CTCATCATCA TCGGATCCTT CTTTGTTCTC AACCTCGTCC TGGGAGTGCT TTCCGGGGAA 
TTTGCCAAAG AAAGAGAGAG AGTGGAGAAC CGAAGAGCGT TCATGAAGCT CCGGCGCCAG 
CAACAGATTG AGCGAGAATT GAACGGCTAC CGTGCCTGGA TAGACAAAGC AGAGGAAGTC 
ATGCTTGCTG AAGAAAATAA AAACTCGGGA ACATCAGCCT TGGAAGTGCT TCGAAGGGCA 
ACCATCAAAA GGAGCCGGAC GGAGGCCATG ACCCGAGACT CCAGCGATGA GCACTGCGTT 
GATATCTCTT CAGTGGGCAC ACCTCTTGCC AGAGCCAGTA TCAAGAGCAC AAAAGTAGAT 
GGGGCCTCCT ATTTCCGGCA CAAGGAACGG CTTCTGCGCA TCTCTATCCG CCACATGGTC 
AAGTCCCAAG TCTTTTACTG GATCGTCCTG AGTGTTGTGG CTCTTAATAC TGCCTGTGTG 
GCCATTGTTC ATCACAACCA GCCACAGTGG CTCACTCACC TCCTCTACTA TGCAGAGTTT 
TTGTTTCTGG GACTCTTCCT GTTGGAGATG TCCCTGAAGA TGTATGGCAT GGGGCCACGC 
CTCTATTTTC ACTCATCCTT CAACTGCTTT GATTTTGGGG TCACAGTGGG CAGCATCTTT 
GAGGTTGTCT GGGCAATCTT CAGACCTGGC ACATCTTTTG GAATCAGTGT CTTACGAGCT 
CTCCGGCTCC TAAGGATATT TAAAATAACC AAGTATTGGG CATCCCTCCG GAATCTGGTT 
GTCTCCTTGA TGAGCTCCAT GAAGTCTATC ATCAGCTTAC TCTTCCTTCT CTTCCTCTTC 
ATCGTTGTCT TTGCTCTCCT GGGAATGCAG CTCTTTGGAG GCAGGTTTAA CTTTAATGAT 
GGGACTCCTT CAGCTAATTT TGACACCTTC CCTGCAGCTA TCATGACAGT GTTCCAGATC 
CTGACAGGGG AAGACTGGAA TGAGGTGATG TACAATGGCA TTCGCTCCCA GGGTGGGGTC 
AGCTCAGGCA TGTGGTCTGC CATCTACTTC ATTGTGCTCA CCTTGTTTGG AAACTACACG 
CTGCTAAATG TATTCTTGGC CATCGCTGTG GACAATCTGG CCAATGCCCA GGAACTGACC 
AAGGATGAGC AGGAGGAAGA AGAAGCCTTC AACCAGAAAC ATGCACTGCA GAAGGCCAAG 
GAGGTCAGCC CGATGTCTGC TCCCAACATG CCTTCCATTG AAAGAGACAG AAGGAGAAGA 
CACCACATGT CGATGTGGGA GCCACGCAGC AGCCACCTGA GGGAGCGGAG GCGCCGGCAC 
CACATGTCTG TGTGGGAACA ACGCACCAGC CAGCTGAGGA GGCACATGCA AATGTCTAGC 
CAAGAAGCCC TCAACAAAGA GGAGGCCCCG CCAATGAACC CCCTCAATCC ACTCAACCCA 
CTGAGTCCTC TCAACCCCCT CAATGCTCAC CCCAGCCTTT ATCGGAGGCC CAGGCCCATT 
GAGGGCCTAG CCCTAGGTCT GGGTCTTGAA AAGTGTGAAG AAGAACGCAT CAGTCGTGGG 
GGCTCCCTCA AGGGGGACAT AGGGGGCCTG ACTAGCGCCC TGGACAACCA GAGGAGCCCC 
TTGTCCTTGG GCAAACGGGA GCCACCGTGG CTGCCTAGAT CCTGTCATGG AAACTGTGAC 
CCAATCCAGC AGGAGGCTGG GGGAGGAGAG ACTGTGGTGA CTTTTGAGGA CCGGGCCAGG 
CACAGGCAGA GTCAGAGGCG AAGCCGGCAT CGCCGAGTCA GGACTGAGGG CAAGGACTCT 
GCCTCTGCAT CCCGAAGCAG GTCTGCCAGC CAGGAGCGGA GTCTGGATGA AGGGGTGTCC 
GTTGAGGGGG AGAAGGAACA TGAGCCTCAC AGCAGCCACA GGAGCAAAGA GCCGACCATC 
CACGAAGAGG AGAGAACCCA GGACTTAAGG AGGACCAACA GTCTGATGGT CCCCAGGGGC 
TCTGGACTGG TAGGGGCTCT TGATGAAGCA GAAACACCTC TAGTCCAGCC CCAACCTGAG 
CTGGAAGTGG GGAAGGATGC AGCTCTGACA GAGCAGGAGG CTGAAGGCAG CAGTGAACAA 
GCCCTTCTTG GGGACGTACA GCTGGATGTG GGCCGGGGCA TTAGCCAGAG TGAACCGGAT 
CTCTCCTGCA TGACAGCCAA CATGGACAAG GCTACCACGG AGAGCACCAG TGTCACGGTC 
GCCATCCCTG ATGTGGACCC CTTGGTGGAC TCGACAGTGG TGAACATTAG CAACAAGACG 
GATGGAGAAG CCAGTCCCTT GAAGGAGGCA GAGACTAAAG AGGAAGAAGA AGAGGTAGAG 
AAGAAGAAGA AGCAGAAGAA GGAGAAGCGA GAGACTGGAA AAGCCATGGT GCCCCATAGC 
TCTATGTTCA TCTTCAGCAC CACTAACCCG ATCCGCAGGG CCTGCCACTA CATTGTGAAC 
CTACGCTACT TTGAAATGTG CATTCTCTTG GTGATTGCGG CCAGCAGCAT TGCCCTGGCA 
GCCGAGGACC CTGTACTAAC TAACTCGGAG CGTAACAAAG TCCTGAGATA TTTCGACTAT 
GTTTTCACAG GAGTGTTTAC CTTTGAGATG GTTATAAAGA TGATAGACCA AGGCTTGATC 
CTGCAGGATG GATCATACTT CAGAGACCTC TGGAACATCC TGGACTTTGT AGTAGTGGTT 
GGTGCATTGG TAGCATTTGC TCTGGCGAAC GCTTTGGGAA CCAACAAGGG GAGGGATATC 
AAGACTATCA AGTCTCTGAG GGTACTTCGA GTTCTGCGGC CACTGAAAAC CATCAAGCGC 
TTGCCCAAGC TGAAGGCTGT GTTTGACTGC GTGGTGACCT CCTTGAAGAA TGTCTTCAAC 
ATACTCATTG TCTACAAGCT CTTCATGTTC ATCTTTGCCG TCATCGCTGT TCAGCTATTC 
AAAGGAAAGT TCTTTTATTG CACTGACAGT TCCAAGGACA CAGAGAAGGA GTGCATAGGC 
AACTATGTAG ACCACGAGAA AAACAAGATG GAGGTGAAGG GCCGGGAATG GAAGCGTCAT 
GAATTCCACT ATGACAACAT CATCTGGGCC CTGCTTACCC TCTTCACAGT CTCTACAGGG 
GAAGGATGGC CTCAGGTTCT GCAGCACTCT GTAGATGTGA CAGAGGAGGA CAGAGGCCCA 
AGTCGCAGCA ACCGCATGGA GATGTCCATC TTTTATGTGG TCTACTTTGT GGTCTTCCCT 
TTCTTCTTTG TCAATATCTT CGTGGCTCTC ATTATCATCA CCTTCCAGGA GCAAGGGGAC 
AAGATGATGG AGGAGTGTAG CCTGGAGAAG AATGAGCGGG CATGCATCGA CTTCGCCATT 
AGTGCCAAAC CTCTCACCCG CTACATGCCA CAGAACAGGC ACACCTTCCA GTACCGTGTG 
TGGCACTTTG TAGTCTCCCC ATCCTTTGAG TATACCATCA TGGCCATGAT TGCCTTGAAC 
ACTGTCGTGC TGATGATGAA GTACTACTCT GCTCCTTGCA CCTATGAGCT CGCCTTAAAG 
TACCTGAACA TCGCCTTCAC TATGGTGTTT TCCCTGGAGT GTGTCCTAAA GGTCATTGCT 
TTCGGCTTCC TGAACTATTT CCGAGATACC TGGAACATCT TTGACTTCAT CACGGTGATC 
GGCAGTATCA CAGAAATTAT CCTGACAGAT AGCAAGCTGG TGAACACCAG TGGCTTCAAT 
ATGAGCTTTC TGAAGCTCTT CCGAGCTGCA CGGCTCATAA AGCTTCTGAG ACAGGGCTAC 
ACCATCCGCA TCTTACTGTG GACCTTCGTG CAGTCCTTTA AGGCTCTTCC CTACGTCTGC 
CTTCTCATTG CCATGCTTTT CTTCATCTAC GCCATCATTG GGATGCAGGT GTTTGGAAAC 
ATAAAGTTAG ATGAGGAGAG TCACATCAAC CGGCACAACA ACTTCCGGAG TTTCTTTGGA 
TCCCTCATGC TGCTTTTCAG GAGTGCCACG GGGGAGGCTT GGCAGGAGAT TATGCTGTCC 
TGTCTCGGTG AGAAGGGTTG TGAGCCTGAC ACCACTGCAC CCTCAGGGCA GAATGAGAGT 
GAGCGCTGTG GCACTGACCT GGCCTACGTT TATTTTGTCT CCTTCATCTT CTTCTGCTCC 
TTCCTGATGC TCAACCTGTT CGTGGCTGTC ATCATGGACA ACTTTGAGTA CCTGACTCGA 
GATTCCTCCA TCCTGGGGCC TCACCACTTA GATGAGTTTG TCCGTGTCTG GGCAGAATAT 
GACAGAGCAG CATGCGGCAG GATCCCGTAT AAGGATATGT ACAAATTAGT GCGGGTGATC 
TCGCCTCCTC TGGGCCTGGG AGAGAACTGC CCCTACAGGG TGGCTTGCAA GAGGCTGGTC 
CTGATGAACA TGCCAGTGGC TGAGGACATG ACAGTCCACT TCACCTCCAC ACTTATGGCT 
CTGATCCGGA CAGCTCTGGA CATTAAAATC GCCAAAGGTG GTGCAGACAG ACAGCAGCTA 
GACTCGGAGC TGCAAAAAGA GACCCTGGCC ATTTGGCCTC ACCTGTCCCA AAAGATGTTG 
GATCTGCTTG TGCCCATGCC CAAAGCCTCT GACCTGACTG TGGGCAAGAT CTATGCAGCA 
ATGATGATCA TGGACTACTA TAAACAGAGT AAGGTAAAGA AACAGAGACA GCAGCTGGAA 
GAACAGAAAA ATGCACCCAT GTTCCAGCGC ATGGAGCCCT CATCACTGCC TCAGGAGATC 
ATTGCTAATG CCAAAGCCCT GCCTTATCTC CAGCAGGACC CTGTTTCTGG CCTGAGTGGT 
CGGAGTGGAT ACCCTTCAAT GAGTCCACTC TCCCCTCAAG AAATATTCCA GTTGGCTTGT 
ATGGACCCAG CGGATGATGG ACAATTCCAG GAACAACAAT CTCTGGTGGT AACAGACCCT 
AGCTCTATGC GACGTTCATT TTCTACAATT CGGGATAAGC GTTCAAATTC CTCCTGGTTG 
GAGGAGTTCT CCATGGAACG CAGCAGTGAA AACACTTACA AGTCGCGGCG CAGAAGTTAC 
CATTCCTCCC TGAGGCTGTC AGCACACCGC CTGAATTCTG ACTCTGGTCA CAAGTCTGAC 
ACTCACCGAT CAGGAGGCAG GGAGAGAGGC CGATCCAAAG AACGAAAGCA CCTCCTCTCT 
CCAGATGTCT CCCGCTGCAA TTCTGAAGAG CGAGGGACCC AGGCCGACTG GGAGTCCCCA 
GAACGTCGCC AGTCCAGGTC ACCCAGTGAA GGAAGGTCAC AGACCCCCAA TAGACAGGGT 
ACCGGTTCCC TAAGTGAGAG CTCCATCCCC TCTATTTCCG ACACCAGCAC CCCAAGACGA 
AGCCGTCGCC AGCTCCCACC TGTGCCACCA AAGCCTCGTC CCCTCCTCTC CTACAGCTCC 
CTGATGAGAC ACACTGGCGG CATCTCTCCA CCTCCTGATG GAAGTGAGGG TGGATCCCCA 
CTGGCCTCTC AAGCTCTGGA GAGCAACAGC GCTTGCCTGA CCGAGTCTTC CAACTCCTTG 
CACCCCCAGC AGGGCCAGCA TCCTTCCCCA CAGCACTACA TCTCCGAGCC CTATCTGGCC 
CTCCATGAAG ACTCCCACGC CTCAGACTGT GGCGAGGAGG AGACACTCAC CTTTGAGGCA 
GCCGTAGCCA CTAGCTTGGG CCGGTCCAAT ACCATTGGCT CTGCGCCACC CCTACGGCAC 
AGCTGGCAGA TGCCTAATGG GCACTATCGG CGGCGGAGGC GTGGGGGGCC TGGGCCTGGC 
ATGATGTGTG GAGCTGTCAG TGACCTCCTG AGTGACACGG AAGAAGACGA TAAGTGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017168255.1
CDS	594..7853
Translation	MVPEMRGLSPGRRLPEPTLCPATGGSPEPGAAGRRRHVLALPDEEAWRPQQCALLEANASDEVGMLPASPRSAVAGFDNFFPVQEGEGPGWEGSTTLDAGSSPFLPVSPEVMKRRRGGLIEQRDIIKAHEAHKMQSTPQARRKEWEMARFGEAVVVGRPGSGDGDSDQSRNRQGTPVPASGPAAAYKQSKAQRARTMALYNPIPVRQNCFTVNRSLFIFGEDNIVRKYAKKLIDWPPFEYMILATIIANCIVLALEQHLPEDDKTPMSRRLEKTEPYFIGIFCFEAGIKIVALGFIFHKGSYLRNGWNVMDFIVVLSGILATAGTHFNTHVDLRTLRAVRVLRPLKLVSGIPSLQIVLKSIMKAMVPLLQIGLLLFFAILMFAIIGLEFYSGKLHRACFMNNSGILEGFDPPHPCGVQGCPAGYECKDWIGPNDGITQFDNILFAVLTVFQCITMEGWTTVLYNTNDALGATWNWLYFIPLIIIGSFFVLNLVLGVLSGEFAKERERVENRRAFMKLRRQQQIERELNGYRAWIDKAEEVMLAEENKNSGTSALEVLRRATIKRSRTEAMTRDSSDEHCVDISSVGTPLARASIKSTKVDGASYFRHKERLLRISIRHMVKSQVFYWIVLSVVALNTACVAIVHHNQPQWLTHLLYYAEFLFLGLFLLEMSLKMYGMGPRLYFHSSFNCFDFGVTVGSIFEVVWAIFRPGTSFGISVLRALRLLRIFKITKYWASLRNLVVSLMSSMKSIISLLFLLFLFIVVFALLGMQLFGGRFNFNDGTPSANFDTFPAAIMTVFQILTGEDWNEVMYNGIRSQGGVSSGMWSAIYFIVLTLFGNYTLLNVFLAIAVDNLANAQELTKDEQEEEEAFNQKHALQKAKEVSPMSAPNMPSIERDRRRRHHMSMWEPRSSHLRERRRRHHMSVWEQRTSQLRRHMQMSSQEALNKEEAPPMNPLNPLNPLSPLNPLNAHPSLYRRPRPIEGLALGLGLEKCEEERISRGGSLKGDIGGLTSALDNQRSPLSLGKREPPWLPRSCHGNCDPIQQEAGGGETVVTFEDRARHRQSQRRSRHRRVRTEGKDSASASRSRSASQERSLDEGVSVEGEKEHEPHSSHRSKEPTIHEEERTQDLRRTNSLMVPRGSGLVGALDEAETPLVQPQPELEVGKDAALTEQEAEGSSEQALLGDVQLDVGRGISQSEPDLSCMTANMDKATTESTSVTVAIPDVDPLVDSTVVNISNKTDGEASPLKEAETKEEEEEVEKKKKQKKEKRETGKAMVPHSSMFIFSTTNPIRRACHYIVNLRYFEMCILLVIAASSIALAAEDPVLTNSERNKVLRYFDYVFTGVFTFEMVIKMIDQGLILQDGSYFRDLWNILDFVVVVGALVAFALANALGTNKGRDIKTIKSLRVLRVLRPLKTIKRLPKLKAVFDCVVTSLKNVFNILIVYKLFMFIFAVIAVQLFKGKFFYCTDSSKDTEKECIGNYVDHEKNKMEVKGREWKRHEFHYDNIIWALLTLFTVSTGEGWPQVLQHSVDVTEEDRGPSRSNRMEMSIFYVVYFVVFPFFFVNIFVALIIITFQEQGDKMMEECSLEKNERACIDFAISAKPLTRYMPQNRHTFQYRVWHFVVSPSFEYTIMAMIALNTVVLMMKYYSAPCTYELALKYLNIAFTMVFSLECVLKVIAFGFLNYFRDTWNIFDFITVIGSITEIILTDSKLVNTSGFNMSFLKLFRAARLIKLLRQGYTIRILLWTFVQSFKALPYVCLLIAMLFFIYAIIGMQVFGNIKLDEESHINRHNNFRSFFGSLMLLFRSATGEAWQEIMLSCLGEKGCEPDTTAPSGQNESERCGTDLAYVYFVSFIFFCSFLMLNLFVAVIMDNFEYLTRDSSILGPHHLDEFVRVWAEYDRAACGRIPYKDMYKLVRVISPPLGLGENCPYRVACKRLVLMNMPVAEDMTVHFTSTLMALIRTALDIKIAKGGADRQQLDSELQKETLAIWPHLSQKMLDLLVPMPKASDLTVGKIYAAMMIMDYYKQSKVKKQRQQLEEQKNAPMFQRMEPSSLPQEIIANAKALPYLQQDPVSGLSGRSGYPSMSPLSPQEIFQLACMDPADDGQFQEQQSLVVTDPSSMRRSFSTIRDKRSNSSWLEEFSMERSSENTYKSRRRSYHSSLRLSAHRLNSDSGHKSDTHRSGGRERGRSKERKHLLSPDVSRCNSEERGTQADWESPERRQSRSPSEGRSQTPNRQGTGSLSESSIPSISDTSTPRRSRRQLPPVPPKPRPLLSYSSLMRHTGGISPPPDGSEGGSPLASQALESNSACLTESSNSLHPQQGQHPSPQHYISEPYLALHEDSHASDCGEEETLTFEAAVATSLGRSNTIGSAPPLRHSWQMPNGHYRRRRRGGPGPGMMCGAVSDLLSDTEEDDKC

Target ORF information:

RefSeq Version	XM_017312766.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus calcium channel, voltage-dependent, R type, alpha 1E subunit (Cacna1e), transcript variant X14, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017312766.1

ORF Insert Sequence:

ATGGTGCCCG AGATGCGTGG ACTGAGCCCA GGCCGAAGGC TGCCCGAGCC CACCCTGTGC 
CCGGCTACAG GAGGGAGCCC TGAGCCCGGG GCTGCTGGCA GGCGCCGCCA CGTCCTGGCT 
CTGCCTGATG AGGAGGCATG GCGGCCGCAG CAGTGCGCCC TGCTCGAGGC CAATGCCTCC 
GACGAGGTGG GCATGTTGCC TGCTTCCCCG CGCTCCGCGG TGGCCGGCTT CGACAACTTC 
TTCCCGGTGC AGGAAGGCGA GGGCCCGGGC TGGGAGGGCA GCACCACGCT GGATGCAGGC 
TCCAGCCCCT TCCTTCCTGT GAGCCCAGAG GTCATGAAGC GGAGGCGAGG GGGGCTCATC 
GAGCAGCGGG ACATCATCAA GGCCCACGAG GCACACAAGA TGCAGAGCAC CCCGCAGGCC 
CGGCGCAAGG AGTGGGAGAT GGCTCGCTTC GGGGAGGCGG TGGTCGTTGG CAGGCCAGGC 
TCAGGCGATG GAGACTCGGA CCAGAGCAGG AACCGACAAG GAACCCCCGT CCCGGCCTCG 
GGGCCGGCGG CCGCCTACAA GCAGTCAAAA GCGCAGAGGG CGCGGACTAT GGCTTTGTAC 
AACCCCATAC CAGTCCGGCA GAACTGTTTC ACGGTCAACA GATCCCTGTT CATCTTCGGA 
GAAGATAACA TTGTCAGGAA ATACGCCAAG AAGCTCATCG ATTGGCCGCC ATTTGAGTAC 
ATGATCCTGG CCACCATCAT TGCCAACTGC ATCGTCTTGG CCCTGGAGCA GCATCTTCCT 
GAGGATGACA AGACCCCAAT GTCTCGAAGA CTGGAGAAGA CAGAACCATA TTTCATTGGG 
ATCTTCTGCT TTGAAGCTGG GATCAAAATT GTGGCTCTAG GGTTCATCTT CCATAAGGGT 
TCATACCTCC GTAATGGCTG GAATGTCATG GACTTCATCG TGGTCCTTAG TGGCATCCTG 
GCCACTGCGG GAACCCACTT CAACACCCAC GTGGACCTGC GGACCCTCCG GGCTGTGCGT 
GTCCTGAGGC CTTTAAAGCT GGTATCAGGA ATACCTAGCC TGCAGATCGT GCTGAAGTCT 
ATCATGAAGG CCATGGTGCC TCTTCTGCAG ATTGGCCTTC TGCTCTTCTT TGCCATCCTC 
ATGTTTGCTA TCATTGGCTT GGAGTTCTAC AGTGGCAAGT TGCATCGAGC ATGCTTCATG 
AACAATTCAG GTATTCTAGA GGGGTTTGAT CCTCCTCACC CGTGTGGTGT GCAAGGCTGC 
CCGGCTGGTT ATGAATGTAA GGACTGGATC GGCCCCAATG ACGGGATCAC CCAGTTTGAC 
AACATCCTTT TTGCCGTGCT GACTGTCTTC CAATGCATCA CTATGGAAGG GTGGACCACT 
GTGCTGTACA ATACCAATGA TGCCTTAGGA GCCACCTGGA ACTGGCTGTA CTTCATCCCT 
CTCATCATCA TCGGATCCTT CTTTGTTCTC AACCTCGTCC TGGGAGTGCT TTCCGGGGAA 
TTTGCCAAAG AAAGAGAGAG AGTGGAGAAC CGAAGAGCGT TCATGAAGCT CCGGCGCCAG 
CAACAGATTG AGCGAGAATT GAACGGCTAC CGTGCCTGGA TAGACAAAGC AGAGGAAGTC 
ATGCTTGCTG AAGAAAATAA AAACTCGGGA ACATCAGCCT TGGAAGTGCT TCGAAGGGCA 
ACCATCAAAA GGAGCCGGAC GGAGGCCATG ACCCGAGACT CCAGCGATGA GCACTGCGTT 
GATATCTCTT CAGTGGGCAC ACCTCTTGCC AGAGCCAGTA TCAAGAGCAC AAAAGTAGAT 
GGGGCCTCCT ATTTCCGGCA CAAGGAACGG CTTCTGCGCA TCTCTATCCG CCACATGGTC 
AAGTCCCAAG TCTTTTACTG GATCGTCCTG AGTGTTGTGG CTCTTAATAC TGCCTGTGTG 
GCCATTGTTC ATCACAACCA GCCACAGTGG CTCACTCACC TCCTCTACTA TGCAGAGTTT 
TTGTTTCTGG GACTCTTCCT GTTGGAGATG TCCCTGAAGA TGTATGGCAT GGGGCCACGC 
CTCTATTTTC ACTCATCCTT CAACTGCTTT GATTTTGGGG TCACAGTGGG CAGCATCTTT 
GAGGTTGTCT GGGCAATCTT CAGACCTGGC ACATCTTTTG GAATCAGTGT CTTACGAGCT 
CTCCGGCTCC TAAGGATATT TAAAATAACC AAGTATTGGG CATCCCTCCG GAATCTGGTT 
GTCTCCTTGA TGAGCTCCAT GAAGTCTATC ATCAGCTTAC TCTTCCTTCT CTTCCTCTTC 
ATCGTTGTCT TTGCTCTCCT GGGAATGCAG CTCTTTGGAG GCAGGTTTAA CTTTAATGAT 
GGGACTCCTT CAGCTAATTT TGACACCTTC CCTGCAGCTA TCATGACAGT GTTCCAGATC 
CTGACAGGGG AAGACTGGAA TGAGGTGATG TACAATGGCA TTCGCTCCCA GGGTGGGGTC 
AGCTCAGGCA TGTGGTCTGC CATCTACTTC ATTGTGCTCA CCTTGTTTGG AAACTACACG 
CTGCTAAATG TATTCTTGGC CATCGCTGTG GACAATCTGG CCAATGCCCA GGAACTGACC 
AAGGATGAGC AGGAGGAAGA AGAAGCCTTC AACCAGAAAC ATGCACTGCA GAAGGCCAAG 
GAGGTCAGCC CGATGTCTGC TCCCAACATG CCTTCCATTG AAAGAGACAG AAGGAGAAGA 
CACCACATGT CGATGTGGGA GCCACGCAGC AGCCACCTGA GGGAGCGGAG GCGCCGGCAC 
CACATGTCTG TGTGGGAACA ACGCACCAGC CAGCTGAGGA GGCACATGCA AATGTCTAGC 
CAAGAAGCCC TCAACAAAGA GGAGGCCCCG CCAATGAACC CCCTCAATCC ACTCAACCCA 
CTGAGTCCTC TCAACCCCCT CAATGCTCAC CCCAGCCTTT ATCGGAGGCC CAGGCCCATT 
GAGGGCCTAG CCCTAGGTCT GGGTCTTGAA AAGTGTGAAG AAGAACGCAT CAGTCGTGGG 
GGCTCCCTCA AGGGGGACAT AGGGGGCCTG ACTAGCGCCC TGGACAACCA GAGGAGCCCC 
TTGTCCTTGG GCAAACGGGA GCCACCGTGG CTGCCTAGAT CCTGTCATGG AAACTGTGAC 
CCAATCCAGC AGGAGGCTGG GGGAGGAGAG ACTGTGGTGA CTTTTGAGGA CCGGGCCAGG 
CACAGGCAGA GTCAGAGGCG AAGCCGGCAT CGCCGAGTCA GGACTGAGGG CAAGGACTCT 
GCCTCTGCAT CCCGAAGCAG GTCTGCCAGC CAGGAGCGGA GTCTGGATGA AGGGGTGTCC 
GTTGAGGGGG AGAAGGAACA TGAGCCTCAC AGCAGCCACA GGAGCAAAGA GCCGACCATC 
CACGAAGAGG AGAGAACCCA GGACTTAAGG AGGACCAACA GTCTGATGGT CCCCAGGGGC 
TCTGGACTGG TAGGGGCTCT TGATGAAGCA GAAACACCTC TAGTCCAGCC CCAACCTGAG 
CTGGAAGTGG GGAAGGATGC AGCTCTGACA GAGCAGGAGG CTGAAGGCAG CAGTGAACAA 
GCCCTTCTTG GGGACGTACA GCTGGATGTG GGCCGGGGCA TTAGCCAGAG TGAACCGGAT 
CTCTCCTGCA TGACAGCCAA CATGGACAAG GCTACCACGG AGAGCACCAG TGTCACGGTC 
GCCATCCCTG ATGTGGACCC CTTGGTGGAC TCGACAGTGG TGAACATTAG CAACAAGACG 
GATGGAGAAG CCAGTCCCTT GAAGGAGGCA GAGACTAAAG AGGAAGAAGA AGAGGTAGAG 
AAGAAGAAGA AGCAGAAGAA GGAGAAGCGA GAGACTGGAA AAGCCATGGT GCCCCATAGC 
TCTATGTTCA TCTTCAGCAC CACTAACCCG ATCCGCAGGG CCTGCCACTA CATTGTGAAC 
CTACGCTACT TTGAAATGTG CATTCTCTTG GTGATTGCGG CCAGCAGCAT TGCCCTGGCA 
GCCGAGGACC CTGTACTAAC TAACTCGGAG CGTAACAAAG TCCTGAGATA TTTCGACTAT 
GTTTTCACAG GAGTGTTTAC CTTTGAGATG GTTATAAAGA TGATAGACCA AGGCTTGATC 
CTGCAGGATG GATCATACTT CAGAGACCTC TGGAACATCC TGGACTTTGT AGTAGTGGTT 
GGTGCATTGG TAGCATTTGC TCTGGCGAAC GCTTTGGGAA CCAACAAGGG GAGGGATATC 
AAGACTATCA AGTCTCTGAG GGTACTTCGA GTTCTGCGGC CACTGAAAAC CATCAAGCGC 
TTGCCCAAGC TGAAGGCTGT GTTTGACTGC GTGGTGACCT CCTTGAAGAA TGTCTTCAAC 
ATACTCATTG TCTACAAGCT CTTCATGTTC ATCTTTGCCG TCATCGCTGT TCAGCTATTC 
AAAGGAAAGT TCTTTTATTG CACTGACAGT TCCAAGGACA CAGAGAAGGA GTGCATAGGC 
AACTATGTAG ACCACGAGAA AAACAAGATG GAGGTGAAGG GCCGGGAATG GAAGCGTCAT 
GAATTCCACT ATGACAACAT CATCTGGGCC CTGCTTACCC TCTTCACAGT CTCTACAGGG 
GAAGGATGGC CTCAGGTTCT GCAGCACTCT GTAGATGTGA CAGAGGAGGA CAGAGGCCCA 
AGTCGCAGCA ACCGCATGGA GATGTCCATC TTTTATGTGG TCTACTTTGT GGTCTTCCCT 
TTCTTCTTTG TCAATATCTT CGTGGCTCTC ATTATCATCA CCTTCCAGGA GCAAGGGGAC 
AAGATGATGG AGGAGTGTAG CCTGGAGAAG AATGAGCGGG CATGCATCGA CTTCGCCATT 
AGTGCCAAAC CTCTCACCCG CTACATGCCA CAGAACAGGC ACACCTTCCA GTACCGTGTG 
TGGCACTTTG TAGTCTCCCC ATCCTTTGAG TATACCATCA TGGCCATGAT TGCCTTGAAC 
ACTGTCGTGC TGATGATGAA GTACTACTCT GCTCCTTGCA CCTATGAGCT CGCCTTAAAG 
TACCTGAACA TCGCCTTCAC TATGGTGTTT TCCCTGGAGT GTGTCCTAAA GGTCATTGCT 
TTCGGCTTCC TGAACTATTT CCGAGATACC TGGAACATCT TTGACTTCAT CACGGTGATC 
GGCAGTATCA CAGAAATTAT CCTGACAGAT AGCAAGCTGG TGAACACCAG TGGCTTCAAT 
ATGAGCTTTC TGAAGCTCTT CCGAGCTGCA CGGCTCATAA AGCTTCTGAG ACAGGGCTAC 
ACCATCCGCA TCTTACTGTG GACCTTCGTG CAGTCCTTTA AGGCTCTTCC CTACGTCTGC 
CTTCTCATTG CCATGCTTTT CTTCATCTAC GCCATCATTG GGATGCAGGT GTTTGGAAAC 
ATAAAGTTAG ATGAGGAGAG TCACATCAAC CGGCACAACA ACTTCCGGAG TTTCTTTGGA 
TCCCTCATGC TGCTTTTCAG GAGTGCCACG GGGGAGGCTT GGCAGGAGAT TATGCTGTCC 
TGTCTCGGTG AGAAGGGTTG TGAGCCTGAC ACCACTGCAC CCTCAGGGCA GAATGAGAGT 
GAGCGCTGTG GCACTGACCT GGCCTACGTT TATTTTGTCT CCTTCATCTT CTTCTGCTCC 
TTCCTGATGC TCAACCTGTT CGTGGCTGTC ATCATGGACA ACTTTGAGTA CCTGACTCGA 
GATTCCTCCA TCCTGGGGCC TCACCACTTA GATGAGTTTG TCCGTGTCTG GGCAGAATAT 
GACAGAGCAG CATGCGGCAG GATCCCGTAT AAGGATATGT ACAAATTAGT GCGGGTGATC 
TCGCCTCCTC TGGGCCTGGG AGAGAACTGC CCCTACAGGG TGGCTTGCAA GAGGCTGGTC 
CTGATGAACA TGCCAGTGGC TGAGGACATG ACAGTCCACT TCACCTCCAC ACTTATGGCT 
CTGATCCGGA CAGCTCTGGA CATTAAAATC GCCAAAGGTG GTGCAGACAG ACAGCAGCTA 
GACTCGGAGC TGCAAAAAGA GACCCTGGCC ATTTGGCCTC ACCTGTCCCA AAAGATGTTG 
GATCTGCTTG TGCCCATGCC CAAAGCCTCT GACCTGACTG TGGGCAAGAT CTATGCAGCA 
ATGATGATCA TGGACTACTA TAAACAGAGT AAGGTAAAGA AACAGAGACA GCAGCTGGAA 
GAACAGAAAA ATGCACCCAT GTTCCAGCGC ATGGAGCCCT CATCACTGCC TCAGGAGATC 
ATTGCTAATG CCAAAGCCCT GCCTTATCTC CAGCAGGACC CTGTTTCTGG CCTGAGTGGT 
CGGAGTGGAT ACCCTTCAAT GAGTCCACTC TCCCCTCAAG AAATATTCCA GTTGGCTTGT 
ATGGACCCAG CGGATGATGG ACAATTCCAG GAACAACAAT CTCTGGTGGT AACAGACCCT 
AGCTCTATGC GACGTTCATT TTCTACAATT CGGGATAAGC GTTCAAATTC CTCCTGGTTG 
GAGGAGTTCT CCATGGAACG CAGCAGTGAA AACACTTACA AGTCGCGGCG CAGAAGTTAC 
CATTCCTCCC TGAGGCTGTC AGCACACCGC CTGAATTCTG ACTCTGGTCA CAAGTCTGAC 
ACTCACCGAT CAGGAGGCAG GGAGAGAGGC CGATCCAAAG AACGAAAGCA CCTCCTCTCT 
CCAGATGTCT CCCGCTGCAA TTCTGAAGAG CGAGGGACCC AGGCCGACTG GGAGTCCCCA 
GAACGTCGCC AGTCCAGGTC ACCCAGTGAA GGAAGGTCAC AGACCCCCAA TAGACAGGGT 
ACCGGTTCCC TAAGTGAGAG CTCCATCCCC TCTATTTCCG ACACCAGCAC CCCAAGACGA 
AGCCGTCGCC AGCTCCCACC TGTGCCACCA AAGCCTCGTC CCCTCCTCTC CTACAGCTCC 
CTGATGAGAC ACACTGGCGG CATCTCTCCA CCTCCTGATG GAAGTGAGGG TGGATCCCCA 
CTGGCCTCTC AAGCTCTGGA GAGCAACAGC GCTTGCCTGA CCGAGTCTTC CAACTCCTTG 
CACCCCCAGC AGGGCCAGCA TCCTTCCCCA CAGCACTACA TCTCCGAGCC CTATCTGGCC 
CTCCATGAAG ACTCCCACGC CTCAGACTGT GGCGAGGAGG AGACACTCAC CTTTGAGGCA 
GCCGTAGCCA CTAGCTTGGG CCGGTCCAAT ACCATTGGCT CTGCGCCACC CCTACGGCAC 
AGCTGGCAGA TGCCTAATGG GCACTATCGG CGGCGGAGGC GTGGGGGGCC TGGGCCTGGC 
ATGATGTGTG GAGCTGTCAG TGACCTCCTG AGTGACACGG AAGAAGACGA TAAGTGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cacna1e ( XM_017312855.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017168344.1 Mus musculus calcium channel, voltage-dependent, R type, alpha 1E subunit (Cacna1e), transcript variant X20, mRNA.

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CloneID	OMu76949
Clone ID Related Accession (Same CDS sequence)	XM_017312855.2 , XM_017312855.1
Accession Version	XM_017312855.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6162bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	voltage-dependent R-type calcium channel subunit alpha-1E isoform X16
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_017312855.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGCCCG AGATGCGTGG ACTGAGCCCA GGCCGAAGGC TGCCCGAGCC CACCCTGTGC 
CCGGCTACAG GAGGGAGCCC TGAGCCCGGG GCTGCTGGCA GGCGCCGCCA CGTCCTGGCT 
CTGCCTGATG AGGAGGCATG GCGGCCGCAG CAGTGCGCCC TGCTCGAGGC CAATGCCTCC 
GACGAGGTGG GCATGTTGCC TGCTTCCCCG CGCTCCGCGG TGGCCGGCTT CGACAACTTC 
TTCCCGGTGC AGGAAGGCGA GGGCCCGGGC TGGGAGGGCA GCACCACGCT GGATGCAGGC 
TCCAGCCCCT TCCTTCCTGT GAGCCCAGAG GTCATGAAGC GGAGGCGAGG GGGGCTCATC 
GAGCAGCGGG ACATCATCAA GGCCCACGAG GCACACAAGA TGCAGAGCAC CCCGCAGGCC 
CGGCGCAAGG AGTGGGAGAT GGCTCGCTTC GGGGAGGCGG TGGTCGTTGG CAGGCCAGGC 
TCAGGCGATG GAGACTCGGA CCAGAGCAGG AACCGACAAG GAACCCCCGT CCCGGCCTCG 
GGGCCGGCGG CCGCCTACAA GCAGTCAAAA GCGCAGAGGG CGCGGACTAT GGCTTTGTAC 
AACCCCATAC CAGTCCGGCA GAACTGTTTC ACGGTCAACA GATCCCTGTT CATCTTCGGA 
GAAGATAACA TTGTCAGGAA ATACGCCAAG AAGCTCATCG ATTGGCCGCC ATTTGAGTAC 
ATGATCCTGG CCACCATCAT TGCCAACTGC ATCGTCTTGG CCCTGGAGCA GCATCTTCCT 
GAGGATGACA AGACCCCAAT GTCTCGAAGA CTGGAGAAGA CAGAACCATA TTTCATTGGG 
ATCTTCTGCT TTGAAGCTGG GATCAAAATT GTGGCTCTAG GGTTCATCTT CCATAAGGGT 
TCATACCTCC GTAATGGCTG GAATGTCATG GACTTCATCG TGGTCCTTAG TGGCATCCTG 
GCCACTGCGG GAACCCACTT CAACACCCAC GTGGACCTGC GGACCCTCCG GGCTGTGCGT 
GTCCTGAGGC CTTTAAAGCT GGTATCAGGA ATACCTAGCC TGCAGATCGT GCTGAAGTCT 
ATCATGAAGG CCATGGTGCC TCTTCTGCAG ATTGGCCTTC TGCTCTTCTT TGCCATCCTC 
ATGTTTGCTA TCATTGGCTT GGAGTTCTAC AGTGGCAAGT TGCATCGAGC ATGCTTCATG 
AACAATTCAG GTATTCTAGA GGGGTTTGAT CCTCCTCACC CGTGTGGTGT GCAAGGCTGC 
CCGGCTGGTT ATGAATGTAA GGACTGGATC GGCCCCAATG ACGGGATCAC CCAGTTTGAC 
AACATCCTTT TTGCCGTGCT GACTGTCTTC CAATGCATCA CTATGGAAGG GTGGACCACT 
GTGCTGTACA ATACCAATGA TGCCTTAGGA GCCACCTGGA ACTGGCTGTA CTTCATCCCT 
CTCATCATCA TCGGATCCTT CTTTGTTCTC AACCTCGTCC TGGGAGTGCT TTCCGGGGAA 
TTTGCCAAAG AAAGAGAGAG AGTGGAGAAC CGAAGAGCGT TCATGAAGCT CCGGCGCCAG 
CAACAGATTG AGCGAGAATT GAACGGCTAC CGTGCCTGGA TAGACAAAGC AGAGGAAGTC 
ATGCTTGCTG AAGAAAATAA AAACTCGGGA ACATCAGCCT TGGAAGTGCT TCGAAGGGCA 
ACCATCAAAA GGAGCCGGAC GGAGGCCATG ACCCGAGACT CCAGCGATGA GCACTGCGTT 
GATATCTCTT CAGTGGGCAC ACCTCTTGCC AGAGCCAGTA TCAAGAGCAC AAAAGTAGAT 
GGGGCCTCCT ATTTCCGGCA CAAGGAACGG CTTCTGCGCA TCTCTATCCG CCACATGGTC 
AAGTCCCAAG TCTTTTACTG GATCGTCCTG AGTGTTGTGG CTCTTAATAC TGCCTGTGTG 
GCCATTGTTC ATCACAACCA GCCACAGTGG CTCACTCACC TCCTCTACTA TGCAGAGTTT 
TTGTTTCTGG GACTCTTCCT GTTGGAGATG TCCCTGAAGA TGTATGGCAT GGGGCCACGC 
CTCTATTTTC ACTCATCCTT CAACTGCTTT GATTTTGGGG TCACAGTGGG CAGCATCTTT 
GAGGTTGTCT GGGCAATCTT CAGACCTGGC ACATCTTTTG GAATCAGTGT CTTACGAGCT 
CTCCGGCTCC TAAGGATATT TAAAATAACC AAGTATTGGG CATCCCTCCG GAATCTGGTT 
GTCTCCTTGA TGAGCTCCAT GAAGTCTATC ATCAGCTTAC TCTTCCTTCT CTTCCTCTTC 
ATCGTTGTCT TTGCTCTCCT GGGAATGCAG CTCTTTGGAG GCAGGTTTAA CTTTAATGAT 
GGGACTCCTT CAGCTAATTT TGACACCTTC CCTGCAGCTA TCATGACAGT GTTCCAGATC 
CTGACAGGGG AAGACTGGAA TGAGGTGATG TACAATGGCA TTCGCTCCCA GGGTGGGGTC 
AGCTCAGGCA TGTGGTCTGC CATCTACTTC ATTGTGCTCA CCTTGTTTGG AAACTACACG 
CTGCTAAATG TATTCTTGGC CATCGCTGTG GACAATCTGG CCAATGCCCA GGAACTGACC 
AAGGATGAGC AGGAGGAAGA AGAAGCCTTC AACCAGAAAC ATGCACTGCA GAAGGCCAAG 
GAGGTCAGCC CGATGTCTGC TCCCAACATG CCTTCCATTG AAAGAGACAG AAGGAGAAGA 
CACCACATGT CGATGTGGGA GCCACGCAGC AGCCACCTGA GGGAGCGGAG GCGCCGGCAC 
CACATGTCTG TGTGGGAACA ACGCACCAGC CAGCTGAGGA GGCACATGCA AATGTCTAGC 
CAAGAAGCCC TCAACAAAGA GGAGGCCCCG CCAATGAACC CCCTCAATCC ACTCAACCCA 
CTGAGTCCTC TCAACCCCCT CAATGCTCAC CCCAGCCTTT ATCGGAGGCC CAGGCCCATT 
GAGGGCCTAG CCCTAGGTCT GGGTCTTGAA AAGTGTGAAG AAGAACGCAT CAGTCGTGGG 
GGCTCCCTCA AGGGGGACAT AGGGGGCCTG ACTAGCGCCC TGGACAACCA GAGGAGCCCC 
TTGTCCTTGG GCAAACGGGA GCCACCGTGG CTGCCTAGAT CCTGTCATGG AAACTGTGAC 
CCAATCCAGC AGGAGGCTGG GGGAGGAGAG ACTGTGGTGA CTTTTGAGGA CCGGGCCAGG 
CACAGGCAGA GTCAGAGGCG AAGCCGGCAT CGCCGAGTCA GGACTGAGGG CAAGGACTCT 
GCCTCTGCAT CCCGAAGCAG GTCTGCCAGC CAGGAGCGGA GTCTGGATGA AGGGGTGTCC 
GTTGAGGGGG AGAAGGAACA TGAGCCTCAC AGCAGCCACA GGAGCAAAGA GCCGACCATC 
CACGAAGAGG AGAGAACCCA GGACTTAAGG AGGACCAACA GTCTGATGGT CCCCAGGGGC 
TCTGGACTGG TAGGGGCTCT TGATGAAGCA GAAACACCTC TAGTCCAGCC CCAACCTGAG 
CTGGAAGTGG GGAAGGATGC AGCTCTGACA GAGCAGGAGG CTGAAGGCAG CAGTGAACAA 
GCCCTTCTTG GGGACGTACA GCTGGATGTG GGCCGGGGCA TTAGCCAGAG TGAACCGGAT 
CTCTCCTGCA TGACAGCCAA CATGGACAAG GCTACCACGG AGAGCACCAG TGTCACGGTC 
GCCATCCCTG ATGTGGACCC CTTGGTGGAC TCGACAGTGG TGAACATTAG CAACAAGACG 
GATGGAGAAG CCAGTCCCTT GAAGGAGGCA GAGACTAAAG AGGAAGAAGA AGAGGTAGAG 
AAGAAGAAGA AGCAGAAGAA GGAGAAGCGA GAGACTGGAA AAGCCATGGT GCCCCATAGC 
TCTATGTTCA TCTTCAGCAC CACTAACCCG ATCCGCAGGG CCTGCCACTA CATTGTGAAC 
CTACGCTACT TTGAAATGTG CATTCTCTTG GTGATTGCGG CCAGCAGCAT TGCCCTGGCA 
GCCGAGGACC CTGTACTAAC TAACTCGGAG CGTAACAAAG TCCTGAGATA TTTCGACTAT 
GTTTTCACAG GAGTGTTTAC CTTTGAGATG GTTATAAAGA TGATAGACCA AGGCTTGATC 
CTGCAGGATG GATCATACTT CAGAGACCTC TGGAACATCC TGGACTTTGT AGTAGTGGTT 
GGTGCATTGG TAGCATTTGC TCTGGCGAAC GCTTTGGGAA CCAACAAGGG GAGGGATATC 
AAGACTATCA AGTCTCTGAG GGTACTTCGA GTTCTGCGGC CACTGAAAAC CATCAAGCGC 
TTGCCCAAGC TGAAGGCTGT GTTTGACTGC GTGGTGACCT CCTTGAAGAA TGTCTTCAAC 
ATACTCATTG TCTACAAGCT CTTCATGTTC ATCTTTGCCG TCATCGCTGT TCAGCTATTC 
AAAGGAAAGT TCTTTTATTG CACTGACAGT TCCAAGGACA CAGAGAAGGA GTGCATAGGC 
AACTATGTAG ACCACGAGAA AAACAAGATG GAGGTGAAGG GCCGGGAATG GAAGCGTCAT 
GAATTCCACT ATGACAACAT CATCTGGGCC CTGCTTACCC TCTTCACAGT CTCTACAGGG 
GAAGGATGGC CTCAGGTTCT GCAGCACTCT GTAGATGTGA CAGAGGAGGA CAGAGGCCCA 
AGTCGCAGCA ACCGCATGGA GATGTCCATC TTTTATGTGG TCTACTTTGT GGTCTTCCCT 
TTCTTCTTTG TCAATATCTT CGTGGCTCTC ATTATCATCA CCTTCCAGGA GCAAGGGGAC 
AAGATGATGG AGGAGTGTAG CCTGGAGAAG AATGAGCGGG CATGCATCGA CTTCGCCATT 
AGTGCCAAAC CTCTCACCCG CTACATGCCA CAGAACAGGC ACACCTTCCA GTACCGTGTG 
TGGCACTTTG TAGTCTCCCC ATCCTTTGAG TATACCATCA TGGCCATGAT TGCCTTGAAC 
ACTGTCGTGC TGATGATGAA GTACTACTCT GCTCCTTGCA CCTATGAGCT CGCCTTAAAG 
TACCTGAACA TCGCCTTCAC TATGGTGTTT TCCCTGGAGT GTGTCCTAAA GGTCATTGCT 
TTCGGCTTCC TGAACTATTT CCGAGATACC TGGAACATCT TTGACTTCAT CACGGTGATC 
GGCAGTATCA CAGAAATTAT CCTGACAGAT AGCAAGCTGG TGAACACCAG TGGCTTCAAT 
ATGAGCTTTC TGAAGCTCTT CCGAGCTGCA CGGCTCATAA AGCTTCTGAG ACAGGGCTAC 
ACCATCCGCA TCTTACTGTG GACCTTCGTG CAGTCCTTTA AGGCTCTTCC CTACGTCTGC 
CTTCTCATTG CCATGCTTTT CTTCATCTAC GCCATCATTG GGATGCAGGT GTTTGGAAAC 
ATAAAGTTAG ATGAGGAGAG TCACATCAAC CGGCACAACA ACTTCCGGAG TTTCTTTGGA 
TCCCTCATGC TGCTTTTCAG GAGTGCCACG GGGGAGGCTT GGCAGGAGAT TATGCTGTCC 
TGTCTCGGTG AGAAGGGTTG TGAGCCTGAC ACCACTGCAC CCTCAGGGCA GAATGAGAGT 
GAGCGCTGTG GCACTGACCT GGCCTACGTT TATTTTGTCT CCTTCATCTT CTTCTGCTCC 
TTCCTGATGC TCAACCTGTT CGTGGCTGTC ATCATGGACA ACTTTGAGTA CCTGACTCGA 
GATTCCTCCA TCCTGGGGCC TCACCACTTA GATGAGTTTG TCCGTGTCTG GGCAGAATAT 
GACAGAGCAG CATGTGGCCG CATCCATTAC ACTGAGATGT ATGAAATGCT GACTCTCATG 
TCACCACCGC TAGGCCTCGG CAAGAGATGT CCCTCCAAAG TGGCCTATAA GAGGCTGGTC 
CTGATGAACA TGCCAGTGGC TGAGGACATG ACAGTCCACT TCACCTCCAC ACTTATGGCT 
CTGATCCGGA CAGCTCTGGA CATTAAAATC GCCAAAGGTG GTGCAGACAG ACAGCAGCTA 
GACTCGGAGC TGCAAAAAGA GACCCTGGCC ATTTGGCCTC ACCTGTCCCA AAAGATGTTG 
GATCTGCTTG TGCCCATGCC CAAAGCCTCT GACCTGACTG TGGGCAAGAT CTATGCAGCA 
ATGATGATCA TGGACTACTA TAAACAGAGT AAGGTAAAGA AACAGAGACA GCAGCTGGAA 
GAACAGGTAA GCACCATTGA AAGCACATTG GATGGGTCAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017168344.1
CDS	695..6856
Translation	MVPEMRGLSPGRRLPEPTLCPATGGSPEPGAAGRRRHVLALPDEEAWRPQQCALLEANASDEVGMLPASPRSAVAGFDNFFPVQEGEGPGWEGSTTLDAGSSPFLPVSPEVMKRRRGGLIEQRDIIKAHEAHKMQSTPQARRKEWEMARFGEAVVVGRPGSGDGDSDQSRNRQGTPVPASGPAAAYKQSKAQRARTMALYNPIPVRQNCFTVNRSLFIFGEDNIVRKYAKKLIDWPPFEYMILATIIANCIVLALEQHLPEDDKTPMSRRLEKTEPYFIGIFCFEAGIKIVALGFIFHKGSYLRNGWNVMDFIVVLSGILATAGTHFNTHVDLRTLRAVRVLRPLKLVSGIPSLQIVLKSIMKAMVPLLQIGLLLFFAILMFAIIGLEFYSGKLHRACFMNNSGILEGFDPPHPCGVQGCPAGYECKDWIGPNDGITQFDNILFAVLTVFQCITMEGWTTVLYNTNDALGATWNWLYFIPLIIIGSFFVLNLVLGVLSGEFAKERERVENRRAFMKLRRQQQIERELNGYRAWIDKAEEVMLAEENKNSGTSALEVLRRATIKRSRTEAMTRDSSDEHCVDISSVGTPLARASIKSTKVDGASYFRHKERLLRISIRHMVKSQVFYWIVLSVVALNTACVAIVHHNQPQWLTHLLYYAEFLFLGLFLLEMSLKMYGMGPRLYFHSSFNCFDFGVTVGSIFEVVWAIFRPGTSFGISVLRALRLLRIFKITKYWASLRNLVVSLMSSMKSIISLLFLLFLFIVVFALLGMQLFGGRFNFNDGTPSANFDTFPAAIMTVFQILTGEDWNEVMYNGIRSQGGVSSGMWSAIYFIVLTLFGNYTLLNVFLAIAVDNLANAQELTKDEQEEEEAFNQKHALQKAKEVSPMSAPNMPSIERDRRRRHHMSMWEPRSSHLRERRRRHHMSVWEQRTSQLRRHMQMSSQEALNKEEAPPMNPLNPLNPLSPLNPLNAHPSLYRRPRPIEGLALGLGLEKCEEERISRGGSLKGDIGGLTSALDNQRSPLSLGKREPPWLPRSCHGNCDPIQQEAGGGETVVTFEDRARHRQSQRRSRHRRVRTEGKDSASASRSRSASQERSLDEGVSVEGEKEHEPHSSHRSKEPTIHEEERTQDLRRTNSLMVPRGSGLVGALDEAETPLVQPQPELEVGKDAALTEQEAEGSSEQALLGDVQLDVGRGISQSEPDLSCMTANMDKATTESTSVTVAIPDVDPLVDSTVVNISNKTDGEASPLKEAETKEEEEEVEKKKKQKKEKRETGKAMVPHSSMFIFSTTNPIRRACHYIVNLRYFEMCILLVIAASSIALAAEDPVLTNSERNKVLRYFDYVFTGVFTFEMVIKMIDQGLILQDGSYFRDLWNILDFVVVVGALVAFALANALGTNKGRDIKTIKSLRVLRVLRPLKTIKRLPKLKAVFDCVVTSLKNVFNILIVYKLFMFIFAVIAVQLFKGKFFYCTDSSKDTEKECIGNYVDHEKNKMEVKGREWKRHEFHYDNIIWALLTLFTVSTGEGWPQVLQHSVDVTEEDRGPSRSNRMEMSIFYVVYFVVFPFFFVNIFVALIIITFQEQGDKMMEECSLEKNERACIDFAISAKPLTRYMPQNRHTFQYRVWHFVVSPSFEYTIMAMIALNTVVLMMKYYSAPCTYELALKYLNIAFTMVFSLECVLKVIAFGFLNYFRDTWNIFDFITVIGSITEIILTDSKLVNTSGFNMSFLKLFRAARLIKLLRQGYTIRILLWTFVQSFKALPYVCLLIAMLFFIYAIIGMQVFGNIKLDEESHINRHNNFRSFFGSLMLLFRSATGEAWQEIMLSCLGEKGCEPDTTAPSGQNESERCGTDLAYVYFVSFIFFCSFLMLNLFVAVIMDNFEYLTRDSSILGPHHLDEFVRVWAEYDRAACGRIHYTEMYEMLTLMSPPLGLGKRCPSKVAYKRLVLMNMPVAEDMTVHFTSTLMALIRTALDIKIAKGGADRQQLDSELQKETLAIWPHLSQKMLDLLVPMPKASDLTVGKIYAAMMIMDYYKQSKVKKQRQQLEEQVSTIESTLDGS

Target ORF information:

RefSeq Version	XM_017312855.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus calcium channel, voltage-dependent, R type, alpha 1E subunit (Cacna1e), transcript variant X20, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017312855.2

ORF Insert Sequence:

ATGGTGCCCG AGATGCGTGG ACTGAGCCCA GGCCGAAGGC TGCCCGAGCC CACCCTGTGC 
CCGGCTACAG GAGGGAGCCC TGAGCCCGGG GCTGCTGGCA GGCGCCGCCA CGTCCTGGCT 
CTGCCTGATG AGGAGGCATG GCGGCCGCAG CAGTGCGCCC TGCTCGAGGC CAATGCCTCC 
GACGAGGTGG GCATGTTGCC TGCTTCCCCG CGCTCCGCGG TGGCCGGCTT CGACAACTTC 
TTCCCGGTGC AGGAAGGCGA GGGCCCGGGC TGGGAGGGCA GCACCACGCT GGATGCAGGC 
TCCAGCCCCT TCCTTCCTGT GAGCCCAGAG GTCATGAAGC GGAGGCGAGG GGGGCTCATC 
GAGCAGCGGG ACATCATCAA GGCCCACGAG GCACACAAGA TGCAGAGCAC CCCGCAGGCC 
CGGCGCAAGG AGTGGGAGAT GGCTCGCTTC GGGGAGGCGG TGGTCGTTGG CAGGCCAGGC 
TCAGGCGATG GAGACTCGGA CCAGAGCAGG AACCGACAAG GAACCCCCGT CCCGGCCTCG 
GGGCCGGCGG CCGCCTACAA GCAGTCAAAA GCGCAGAGGG CGCGGACTAT GGCTTTGTAC 
AACCCCATAC CAGTCCGGCA GAACTGTTTC ACGGTCAACA GATCCCTGTT CATCTTCGGA 
GAAGATAACA TTGTCAGGAA ATACGCCAAG AAGCTCATCG ATTGGCCGCC ATTTGAGTAC 
ATGATCCTGG CCACCATCAT TGCCAACTGC ATCGTCTTGG CCCTGGAGCA GCATCTTCCT 
GAGGATGACA AGACCCCAAT GTCTCGAAGA CTGGAGAAGA CAGAACCATA TTTCATTGGG 
ATCTTCTGCT TTGAAGCTGG GATCAAAATT GTGGCTCTAG GGTTCATCTT CCATAAGGGT 
TCATACCTCC GTAATGGCTG GAATGTCATG GACTTCATCG TGGTCCTTAG TGGCATCCTG 
GCCACTGCGG GAACCCACTT CAACACCCAC GTGGACCTGC GGACCCTCCG GGCTGTGCGT 
GTCCTGAGGC CTTTAAAGCT GGTATCAGGA ATACCTAGCC TGCAGATCGT GCTGAAGTCT 
ATCATGAAGG CCATGGTGCC TCTTCTGCAG ATTGGCCTTC TGCTCTTCTT TGCCATCCTC 
ATGTTTGCTA TCATTGGCTT GGAGTTCTAC AGTGGCAAGT TGCATCGAGC ATGCTTCATG 
AACAATTCAG GTATTCTAGA GGGGTTTGAT CCTCCTCACC CGTGTGGTGT GCAAGGCTGC 
CCGGCTGGTT ATGAATGTAA GGACTGGATC GGCCCCAATG ACGGGATCAC CCAGTTTGAC 
AACATCCTTT TTGCCGTGCT GACTGTCTTC CAATGCATCA CTATGGAAGG GTGGACCACT 
GTGCTGTACA ATACCAATGA TGCCTTAGGA GCCACCTGGA ACTGGCTGTA CTTCATCCCT 
CTCATCATCA TCGGATCCTT CTTTGTTCTC AACCTCGTCC TGGGAGTGCT TTCCGGGGAA 
TTTGCCAAAG AAAGAGAGAG AGTGGAGAAC CGAAGAGCGT TCATGAAGCT CCGGCGCCAG 
CAACAGATTG AGCGAGAATT GAACGGCTAC CGTGCCTGGA TAGACAAAGC AGAGGAAGTC 
ATGCTTGCTG AAGAAAATAA AAACTCGGGA ACATCAGCCT TGGAAGTGCT TCGAAGGGCA 
ACCATCAAAA GGAGCCGGAC GGAGGCCATG ACCCGAGACT CCAGCGATGA GCACTGCGTT 
GATATCTCTT CAGTGGGCAC ACCTCTTGCC AGAGCCAGTA TCAAGAGCAC AAAAGTAGAT 
GGGGCCTCCT ATTTCCGGCA CAAGGAACGG CTTCTGCGCA TCTCTATCCG CCACATGGTC 
AAGTCCCAAG TCTTTTACTG GATCGTCCTG AGTGTTGTGG CTCTTAATAC TGCCTGTGTG 
GCCATTGTTC ATCACAACCA GCCACAGTGG CTCACTCACC TCCTCTACTA TGCAGAGTTT 
TTGTTTCTGG GACTCTTCCT GTTGGAGATG TCCCTGAAGA TGTATGGCAT GGGGCCACGC 
CTCTATTTTC ACTCATCCTT CAACTGCTTT GATTTTGGGG TCACAGTGGG CAGCATCTTT 
GAGGTTGTCT GGGCAATCTT CAGACCTGGC ACATCTTTTG GAATCAGTGT CTTACGAGCT 
CTCCGGCTCC TAAGGATATT TAAAATAACC AAGTATTGGG CATCCCTCCG GAATCTGGTT 
GTCTCCTTGA TGAGCTCCAT GAAGTCTATC ATCAGCTTAC TCTTCCTTCT CTTCCTCTTC 
ATCGTTGTCT TTGCTCTCCT GGGAATGCAG CTCTTTGGAG GCAGGTTTAA CTTTAATGAT 
GGGACTCCTT CAGCTAATTT TGACACCTTC CCTGCAGCTA TCATGACAGT GTTCCAGATC 
CTGACAGGGG AAGACTGGAA TGAGGTGATG TACAATGGCA TTCGCTCCCA GGGTGGGGTC 
AGCTCAGGCA TGTGGTCTGC CATCTACTTC ATTGTGCTCA CCTTGTTTGG AAACTACACG 
CTGCTAAATG TATTCTTGGC CATCGCTGTG GACAATCTGG CCAATGCCCA GGAACTGACC 
AAGGATGAGC AGGAGGAAGA AGAAGCCTTC AACCAGAAAC ATGCACTGCA GAAGGCCAAG 
GAGGTCAGCC CGATGTCTGC TCCCAACATG CCTTCCATTG AAAGAGACAG AAGGAGAAGA 
CACCACATGT CGATGTGGGA GCCACGCAGC AGCCACCTGA GGGAGCGGAG GCGCCGGCAC 
CACATGTCTG TGTGGGAACA ACGCACCAGC CAGCTGAGGA GGCACATGCA AATGTCTAGC 
CAAGAAGCCC TCAACAAAGA GGAGGCCCCG CCAATGAACC CCCTCAATCC ACTCAACCCA 
CTGAGTCCTC TCAACCCCCT CAATGCTCAC CCCAGCCTTT ATCGGAGGCC CAGGCCCATT 
GAGGGCCTAG CCCTAGGTCT GGGTCTTGAA AAGTGTGAAG AAGAACGCAT CAGTCGTGGG 
GGCTCCCTCA AGGGGGACAT AGGGGGCCTG ACTAGCGCCC TGGACAACCA GAGGAGCCCC 
TTGTCCTTGG GCAAACGGGA GCCACCGTGG CTGCCTAGAT CCTGTCATGG AAACTGTGAC 
CCAATCCAGC AGGAGGCTGG GGGAGGAGAG ACTGTGGTGA CTTTTGAGGA CCGGGCCAGG 
CACAGGCAGA GTCAGAGGCG AAGCCGGCAT CGCCGAGTCA GGACTGAGGG CAAGGACTCT 
GCCTCTGCAT CCCGAAGCAG GTCTGCCAGC CAGGAGCGGA GTCTGGATGA AGGGGTGTCC 
GTTGAGGGGG AGAAGGAACA TGAGCCTCAC AGCAGCCACA GGAGCAAAGA GCCGACCATC 
CACGAAGAGG AGAGAACCCA GGACTTAAGG AGGACCAACA GTCTGATGGT CCCCAGGGGC 
TCTGGACTGG TAGGGGCTCT TGATGAAGCA GAAACACCTC TAGTCCAGCC CCAACCTGAG 
CTGGAAGTGG GGAAGGATGC AGCTCTGACA GAGCAGGAGG CTGAAGGCAG CAGTGAACAA 
GCCCTTCTTG GGGACGTACA GCTGGATGTG GGCCGGGGCA TTAGCCAGAG TGAACCGGAT 
CTCTCCTGCA TGACAGCCAA CATGGACAAG GCTACCACGG AGAGCACCAG TGTCACGGTC 
GCCATCCCTG ATGTGGACCC CTTGGTGGAC TCGACAGTGG TGAACATTAG CAACAAGACG 
GATGGAGAAG CCAGTCCCTT GAAGGAGGCA GAGACTAAAG AGGAAGAAGA AGAGGTAGAG 
AAGAAGAAGA AGCAGAAGAA GGAGAAGCGA GAGACTGGAA AAGCCATGGT GCCCCATAGC 
TCTATGTTCA TCTTCAGCAC CACTAACCCG ATCCGCAGGG CCTGCCACTA CATTGTGAAC 
CTACGCTACT TTGAAATGTG CATTCTCTTG GTGATTGCGG CCAGCAGCAT TGCCCTGGCA 
GCCGAGGACC CTGTACTAAC TAACTCGGAG CGTAACAAAG TCCTGAGATA TTTCGACTAT 
GTTTTCACAG GAGTGTTTAC CTTTGAGATG GTTATAAAGA TGATAGACCA AGGCTTGATC 
CTGCAGGATG GATCATACTT CAGAGACCTC TGGAACATCC TGGACTTTGT AGTAGTGGTT 
GGTGCATTGG TAGCATTTGC TCTGGCGAAC GCTTTGGGAA CCAACAAGGG GAGGGATATC 
AAGACTATCA AGTCTCTGAG GGTACTTCGA GTTCTGCGGC CACTGAAAAC CATCAAGCGC 
TTGCCCAAGC TGAAGGCTGT GTTTGACTGC GTGGTGACCT CCTTGAAGAA TGTCTTCAAC 
ATACTCATTG TCTACAAGCT CTTCATGTTC ATCTTTGCCG TCATCGCTGT TCAGCTATTC 
AAAGGAAAGT TCTTTTATTG CACTGACAGT TCCAAGGACA CAGAGAAGGA GTGCATAGGC 
AACTATGTAG ACCACGAGAA AAACAAGATG GAGGTGAAGG GCCGGGAATG GAAGCGTCAT 
GAATTCCACT ATGACAACAT CATCTGGGCC CTGCTTACCC TCTTCACAGT CTCTACAGGG 
GAAGGATGGC CTCAGGTTCT GCAGCACTCT GTAGATGTGA CAGAGGAGGA CAGAGGCCCA 
AGTCGCAGCA ACCGCATGGA GATGTCCATC TTTTATGTGG TCTACTTTGT GGTCTTCCCT 
TTCTTCTTTG TCAATATCTT CGTGGCTCTC ATTATCATCA CCTTCCAGGA GCAAGGGGAC 
AAGATGATGG AGGAGTGTAG CCTGGAGAAG AATGAGCGGG CATGCATCGA CTTCGCCATT 
AGTGCCAAAC CTCTCACCCG CTACATGCCA CAGAACAGGC ACACCTTCCA GTACCGTGTG 
TGGCACTTTG TAGTCTCCCC ATCCTTTGAG TATACCATCA TGGCCATGAT TGCCTTGAAC 
ACTGTCGTGC TGATGATGAA GTACTACTCT GCTCCTTGCA CCTATGAGCT CGCCTTAAAG 
TACCTGAACA TCGCCTTCAC TATGGTGTTT TCCCTGGAGT GTGTCCTAAA GGTCATTGCT 
TTCGGCTTCC TGAACTATTT CCGAGATACC TGGAACATCT TTGACTTCAT CACGGTGATC 
GGCAGTATCA CAGAAATTAT CCTGACAGAT AGCAAGCTGG TGAACACCAG TGGCTTCAAT 
ATGAGCTTTC TGAAGCTCTT CCGAGCTGCA CGGCTCATAA AGCTTCTGAG ACAGGGCTAC 
ACCATCCGCA TCTTACTGTG GACCTTCGTG CAGTCCTTTA AGGCTCTTCC CTACGTCTGC 
CTTCTCATTG CCATGCTTTT CTTCATCTAC GCCATCATTG GGATGCAGGT GTTTGGAAAC 
ATAAAGTTAG ATGAGGAGAG TCACATCAAC CGGCACAACA ACTTCCGGAG TTTCTTTGGA 
TCCCTCATGC TGCTTTTCAG GAGTGCCACG GGGGAGGCTT GGCAGGAGAT TATGCTGTCC 
TGTCTCGGTG AGAAGGGTTG TGAGCCTGAC ACCACTGCAC CCTCAGGGCA GAATGAGAGT 
GAGCGCTGTG GCACTGACCT GGCCTACGTT TATTTTGTCT CCTTCATCTT CTTCTGCTCC 
TTCCTGATGC TCAACCTGTT CGTGGCTGTC ATCATGGACA ACTTTGAGTA CCTGACTCGA 
GATTCCTCCA TCCTGGGGCC TCACCACTTA GATGAGTTTG TCCGTGTCTG GGCAGAATAT 
GACAGAGCAG CATGTGGCCG CATCCATTAC ACTGAGATGT ATGAAATGCT GACTCTCATG 
TCACCACCGC TAGGCCTCGG CAAGAGATGT CCCTCCAAAG TGGCCTATAA GAGGCTGGTC 
CTGATGAACA TGCCAGTGGC TGAGGACATG ACAGTCCACT TCACCTCCAC ACTTATGGCT 
CTGATCCGGA CAGCTCTGGA CATTAAAATC GCCAAAGGTG GTGCAGACAG ACAGCAGCTA 
GACTCGGAGC TGCAAAAAGA GACCCTGGCC ATTTGGCCTC ACCTGTCCCA AAAGATGTTG 
GATCTGCTTG TGCCCATGCC CAAAGCCTCT GACCTGACTG TGGGCAAGAT CTATGCAGCA 
ATGATGATCA TGGACTACTA TAAACAGAGT AAGGTAAAGA AACAGAGACA GCAGCTGGAA 
GAACAGGTAA GCACCATTGA AAGCACATTG GATGGGTCAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cacna1e ( XM_017312855.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017168344.1 Mus musculus calcium channel, voltage-dependent, R type, alpha 1E subunit (Cacna1e), transcript variant X18, mRNA.

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CloneID	OMu76949
Clone ID Related Accession (Same CDS sequence)	XM_017312855.2 , XM_017312855.1
Accession Version	XM_017312855.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6162bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	voltage-dependent R-type calcium channel subunit alpha-1E isoform X15
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGCCCG AGATGCGTGG ACTGAGCCCA GGCCGAAGGC TGCCCGAGCC CACCCTGTGC 
CCGGCTACAG GAGGGAGCCC TGAGCCCGGG GCTGCTGGCA GGCGCCGCCA CGTCCTGGCT 
CTGCCTGATG AGGAGGCATG GCGGCCGCAG CAGTGCGCCC TGCTCGAGGC CAATGCCTCC 
GACGAGGTGG GCATGTTGCC TGCTTCCCCG CGCTCCGCGG TGGCCGGCTT CGACAACTTC 
TTCCCGGTGC AGGAAGGCGA GGGCCCGGGC TGGGAGGGCA GCACCACGCT GGATGCAGGC 
TCCAGCCCCT TCCTTCCTGT GAGCCCAGAG GTCATGAAGC GGAGGCGAGG GGGGCTCATC 
GAGCAGCGGG ACATCATCAA GGCCCACGAG GCACACAAGA TGCAGAGCAC CCCGCAGGCC 
CGGCGCAAGG AGTGGGAGAT GGCTCGCTTC GGGGAGGCGG TGGTCGTTGG CAGGCCAGGC 
TCAGGCGATG GAGACTCGGA CCAGAGCAGG AACCGACAAG GAACCCCCGT CCCGGCCTCG 
GGGCCGGCGG CCGCCTACAA GCAGTCAAAA GCGCAGAGGG CGCGGACTAT GGCTTTGTAC 
AACCCCATAC CAGTCCGGCA GAACTGTTTC ACGGTCAACA GATCCCTGTT CATCTTCGGA 
GAAGATAACA TTGTCAGGAA ATACGCCAAG AAGCTCATCG ATTGGCCGCC ATTTGAGTAC 
ATGATCCTGG CCACCATCAT TGCCAACTGC ATCGTCTTGG CCCTGGAGCA GCATCTTCCT 
GAGGATGACA AGACCCCAAT GTCTCGAAGA CTGGAGAAGA CAGAACCATA TTTCATTGGG 
ATCTTCTGCT TTGAAGCTGG GATCAAAATT GTGGCTCTAG GGTTCATCTT CCATAAGGGT 
TCATACCTCC GTAATGGCTG GAATGTCATG GACTTCATCG TGGTCCTTAG TGGCATCCTG 
GCCACTGCGG GAACCCACTT CAACACCCAC GTGGACCTGC GGACCCTCCG GGCTGTGCGT 
GTCCTGAGGC CTTTAAAGCT GGTATCAGGA ATACCTAGCC TGCAGATCGT GCTGAAGTCT 
ATCATGAAGG CCATGGTGCC TCTTCTGCAG ATTGGCCTTC TGCTCTTCTT TGCCATCCTC 
ATGTTTGCTA TCATTGGCTT GGAGTTCTAC AGTGGCAAGT TGCATCGAGC ATGCTTCATG 
AACAATTCAG GTATTCTAGA GGGGTTTGAT CCTCCTCACC CGTGTGGTGT GCAAGGCTGC 
CCGGCTGGTT ATGAATGTAA GGACTGGATC GGCCCCAATG ACGGGATCAC CCAGTTTGAC 
AACATCCTTT TTGCCGTGCT GACTGTCTTC CAATGCATCA CTATGGAAGG GTGGACCACT 
GTGCTGTACA ATACCAATGA TGCCTTAGGA GCCACCTGGA ACTGGCTGTA CTTCATCCCT 
CTCATCATCA TCGGATCCTT CTTTGTTCTC AACCTCGTCC TGGGAGTGCT TTCCGGGGAA 
TTTGCCAAAG AAAGAGAGAG AGTGGAGAAC CGAAGAGCGT TCATGAAGCT CCGGCGCCAG 
CAACAGATTG AGCGAGAATT GAACGGCTAC CGTGCCTGGA TAGACAAAGC AGAGGAAGTC 
ATGCTTGCTG AAGAAAATAA AAACTCGGGA ACATCAGCCT TGGAAGTGCT TCGAAGGGCA 
ACCATCAAAA GGAGCCGGAC GGAGGCCATG ACCCGAGACT CCAGCGATGA GCACTGCGTT 
GATATCTCTT CAGTGGGCAC ACCTCTTGCC AGAGCCAGTA TCAAGAGCAC AAAAGTAGAT 
GGGGCCTCCT ATTTCCGGCA CAAGGAACGG CTTCTGCGCA TCTCTATCCG CCACATGGTC 
AAGTCCCAAG TCTTTTACTG GATCGTCCTG AGTGTTGTGG CTCTTAATAC TGCCTGTGTG 
GCCATTGTTC ATCACAACCA GCCACAGTGG CTCACTCACC TCCTCTACTA TGCAGAGTTT 
TTGTTTCTGG GACTCTTCCT GTTGGAGATG TCCCTGAAGA TGTATGGCAT GGGGCCACGC 
CTCTATTTTC ACTCATCCTT CAACTGCTTT GATTTTGGGG TCACAGTGGG CAGCATCTTT 
GAGGTTGTCT GGGCAATCTT CAGACCTGGC ACATCTTTTG GAATCAGTGT CTTACGAGCT 
CTCCGGCTCC TAAGGATATT TAAAATAACC AAGTATTGGG CATCCCTCCG GAATCTGGTT 
GTCTCCTTGA TGAGCTCCAT GAAGTCTATC ATCAGCTTAC TCTTCCTTCT CTTCCTCTTC 
ATCGTTGTCT TTGCTCTCCT GGGAATGCAG CTCTTTGGAG GCAGGTTTAA CTTTAATGAT 
GGGACTCCTT CAGCTAATTT TGACACCTTC CCTGCAGCTA TCATGACAGT GTTCCAGATC 
CTGACAGGGG AAGACTGGAA TGAGGTGATG TACAATGGCA TTCGCTCCCA GGGTGGGGTC 
AGCTCAGGCA TGTGGTCTGC CATCTACTTC ATTGTGCTCA CCTTGTTTGG AAACTACACG 
CTGCTAAATG TATTCTTGGC CATCGCTGTG GACAATCTGG CCAATGCCCA GGAACTGACC 
AAGGATGAGC AGGAGGAAGA AGAAGCCTTC AACCAGAAAC ATGCACTGCA GAAGGCCAAG 
GAGGTCAGCC CGATGTCTGC TCCCAACATG CCTTCCATTG AAAGAGACAG AAGGAGAAGA 
CACCACATGT CGATGTGGGA GCCACGCAGC AGCCACCTGA GGGAGCGGAG GCGCCGGCAC 
CACATGTCTG TGTGGGAACA ACGCACCAGC CAGCTGAGGA GGCACATGCA AATGTCTAGC 
CAAGAAGCCC TCAACAAAGA GGAGGCCCCG CCAATGAACC CCCTCAATCC ACTCAACCCA 
CTGAGTCCTC TCAACCCCCT CAATGCTCAC CCCAGCCTTT ATCGGAGGCC CAGGCCCATT 
GAGGGCCTAG CCCTAGGTCT GGGTCTTGAA AAGTGTGAAG AAGAACGCAT CAGTCGTGGG 
GGCTCCCTCA AGGGGGACAT AGGGGGCCTG ACTAGCGCCC TGGACAACCA GAGGAGCCCC 
TTGTCCTTGG GCAAACGGGA GCCACCGTGG CTGCCTAGAT CCTGTCATGG AAACTGTGAC 
CCAATCCAGC AGGAGGCTGG GGGAGGAGAG ACTGTGGTGA CTTTTGAGGA CCGGGCCAGG 
CACAGGCAGA GTCAGAGGCG AAGCCGGCAT CGCCGAGTCA GGACTGAGGG CAAGGACTCT 
GCCTCTGCAT CCCGAAGCAG GTCTGCCAGC CAGGAGCGGA GTCTGGATGA AGGGGTGTCC 
GTTGAGGGGG AGAAGGAACA TGAGCCTCAC AGCAGCCACA GGAGCAAAGA GCCGACCATC 
CACGAAGAGG AGAGAACCCA GGACTTAAGG AGGACCAACA GTCTGATGGT CCCCAGGGGC 
TCTGGACTGG TAGGGGCTCT TGATGAAGCA GAAACACCTC TAGTCCAGCC CCAACCTGAG 
CTGGAAGTGG GGAAGGATGC AGCTCTGACA GAGCAGGAGG CTGAAGGCAG CAGTGAACAA 
GCCCTTCTTG GGGACGTACA GCTGGATGTG GGCCGGGGCA TTAGCCAGAG TGAACCGGAT 
CTCTCCTGCA TGACAGCCAA CATGGACAAG GCTACCACGG AGAGCACCAG TGTCACGGTC 
GCCATCCCTG ATGTGGACCC CTTGGTGGAC TCGACAGTGG TGAACATTAG CAACAAGACG 
GATGGAGAAG CCAGTCCCTT GAAGGAGGCA GAGACTAAAG AGGAAGAAGA AGAGGTAGAG 
AAGAAGAAGA AGCAGAAGAA GGAGAAGCGA GAGACTGGAA AAGCCATGGT GCCCCATAGC 
TCTATGTTCA TCTTCAGCAC CACTAACCCG ATCCGCAGGG CCTGCCACTA CATTGTGAAC 
CTACGCTACT TTGAAATGTG CATTCTCTTG GTGATTGCGG CCAGCAGCAT TGCCCTGGCA 
GCCGAGGACC CTGTACTAAC TAACTCGGAG CGTAACAAAG TCCTGAGATA TTTCGACTAT 
GTTTTCACAG GAGTGTTTAC CTTTGAGATG GTTATAAAGA TGATAGACCA AGGCTTGATC 
CTGCAGGATG GATCATACTT CAGAGACCTC TGGAACATCC TGGACTTTGT AGTAGTGGTT 
GGTGCATTGG TAGCATTTGC TCTGGCGAAC GCTTTGGGAA CCAACAAGGG GAGGGATATC 
AAGACTATCA AGTCTCTGAG GGTACTTCGA GTTCTGCGGC CACTGAAAAC CATCAAGCGC 
TTGCCCAAGC TGAAGGCTGT GTTTGACTGC GTGGTGACCT CCTTGAAGAA TGTCTTCAAC 
ATACTCATTG TCTACAAGCT CTTCATGTTC ATCTTTGCCG TCATCGCTGT TCAGCTATTC 
AAAGGAAAGT TCTTTTATTG CACTGACAGT TCCAAGGACA CAGAGAAGGA GTGCATAGGC 
AACTATGTAG ACCACGAGAA AAACAAGATG GAGGTGAAGG GCCGGGAATG GAAGCGTCAT 
GAATTCCACT ATGACAACAT CATCTGGGCC CTGCTTACCC TCTTCACAGT CTCTACAGGG 
GAAGGATGGC CTCAGGTTCT GCAGCACTCT GTAGATGTGA CAGAGGAGGA CAGAGGCCCA 
AGTCGCAGCA ACCGCATGGA GATGTCCATC TTTTATGTGG TCTACTTTGT GGTCTTCCCT 
TTCTTCTTTG TCAATATCTT CGTGGCTCTC ATTATCATCA CCTTCCAGGA GCAAGGGGAC 
AAGATGATGG AGGAGTGTAG CCTGGAGAAG AATGAGCGGG CATGCATCGA CTTCGCCATT 
AGTGCCAAAC CTCTCACCCG CTACATGCCA CAGAACAGGC ACACCTTCCA GTACCGTGTG 
TGGCACTTTG TAGTCTCCCC ATCCTTTGAG TATACCATCA TGGCCATGAT TGCCTTGAAC 
ACTGTCGTGC TGATGATGAA GTACTACTCT GCTCCTTGCA CCTATGAGCT CGCCTTAAAG 
TACCTGAACA TCGCCTTCAC TATGGTGTTT TCCCTGGAGT GTGTCCTAAA GGTCATTGCT 
TTCGGCTTCC TGAACTATTT CCGAGATACC TGGAACATCT TTGACTTCAT CACGGTGATC 
GGCAGTATCA CAGAAATTAT CCTGACAGAT AGCAAGCTGG TGAACACCAG TGGCTTCAAT 
ATGAGCTTTC TGAAGCTCTT CCGAGCTGCA CGGCTCATAA AGCTTCTGAG ACAGGGCTAC 
ACCATCCGCA TCTTACTGTG GACCTTCGTG CAGTCCTTTA AGGCTCTTCC CTACGTCTGC 
CTTCTCATTG CCATGCTTTT CTTCATCTAC GCCATCATTG GGATGCAGGT GTTTGGAAAC 
ATAAAGTTAG ATGAGGAGAG TCACATCAAC CGGCACAACA ACTTCCGGAG TTTCTTTGGA 
TCCCTCATGC TGCTTTTCAG GAGTGCCACG GGGGAGGCTT GGCAGGAGAT TATGCTGTCC 
TGTCTCGGTG AGAAGGGTTG TGAGCCTGAC ACCACTGCAC CCTCAGGGCA GAATGAGAGT 
GAGCGCTGTG GCACTGACCT GGCCTACGTT TATTTTGTCT CCTTCATCTT CTTCTGCTCC 
TTCCTGATGC TCAACCTGTT CGTGGCTGTC ATCATGGACA ACTTTGAGTA CCTGACTCGA 
GATTCCTCCA TCCTGGGGCC TCACCACTTA GATGAGTTTG TCCGTGTCTG GGCAGAATAT 
GACAGAGCAG CATGTGGCCG CATCCATTAC ACTGAGATGT ATGAAATGCT GACTCTCATG 
TCACCACCGC TAGGCCTCGG CAAGAGATGT CCCTCCAAAG TGGCCTATAA GAGGCTGGTC 
CTGATGAACA TGCCAGTGGC TGAGGACATG ACAGTCCACT TCACCTCCAC ACTTATGGCT 
CTGATCCGGA CAGCTCTGGA CATTAAAATC GCCAAAGGTG GTGCAGACAG ACAGCAGCTA 
GACTCGGAGC TGCAAAAAGA GACCCTGGCC ATTTGGCCTC ACCTGTCCCA AAAGATGTTG 
GATCTGCTTG TGCCCATGCC CAAAGCCTCT GACCTGACTG TGGGCAAGAT CTATGCAGCA 
ATGATGATCA TGGACTACTA TAAACAGAGT AAGGTAAAGA AACAGAGACA GCAGCTGGAA 
GAACAGGTAA GCACCATTGA AAGCACATTG GATGGGTCAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017168344.1
CDS	696..6857
Translation	MVPEMRGLSPGRRLPEPTLCPATGGSPEPGAAGRRRHVLALPDEEAWRPQQCALLEANASDEVGMLPASPRSAVAGFDNFFPVQEGEGPGWEGSTTLDAGSSPFLPVSPEVMKRRRGGLIEQRDIIKAHEAHKMQSTPQARRKEWEMARFGEAVVVGRPGSGDGDSDQSRNRQGTPVPASGPAAAYKQSKAQRARTMALYNPIPVRQNCFTVNRSLFIFGEDNIVRKYAKKLIDWPPFEYMILATIIANCIVLALEQHLPEDDKTPMSRRLEKTEPYFIGIFCFEAGIKIVALGFIFHKGSYLRNGWNVMDFIVVLSGILATAGTHFNTHVDLRTLRAVRVLRPLKLVSGIPSLQIVLKSIMKAMVPLLQIGLLLFFAILMFAIIGLEFYSGKLHRACFMNNSGILEGFDPPHPCGVQGCPAGYECKDWIGPNDGITQFDNILFAVLTVFQCITMEGWTTVLYNTNDALGATWNWLYFIPLIIIGSFFVLNLVLGVLSGEFAKERERVENRRAFMKLRRQQQIERELNGYRAWIDKAEEVMLAEENKNSGTSALEVLRRATIKRSRTEAMTRDSSDEHCVDISSVGTPLARASIKSTKVDGASYFRHKERLLRISIRHMVKSQVFYWIVLSVVALNTACVAIVHHNQPQWLTHLLYYAEFLFLGLFLLEMSLKMYGMGPRLYFHSSFNCFDFGVTVGSIFEVVWAIFRPGTSFGISVLRALRLLRIFKITKYWASLRNLVVSLMSSMKSIISLLFLLFLFIVVFALLGMQLFGGRFNFNDGTPSANFDTFPAAIMTVFQILTGEDWNEVMYNGIRSQGGVSSGMWSAIYFIVLTLFGNYTLLNVFLAIAVDNLANAQELTKDEQEEEEAFNQKHALQKAKEVSPMSAPNMPSIERDRRRRHHMSMWEPRSSHLRERRRRHHMSVWEQRTSQLRRHMQMSSQEALNKEEAPPMNPLNPLNPLSPLNPLNAHPSLYRRPRPIEGLALGLGLEKCEEERISRGGSLKGDIGGLTSALDNQRSPLSLGKREPPWLPRSCHGNCDPIQQEAGGGETVVTFEDRARHRQSQRRSRHRRVRTEGKDSASASRSRSASQERSLDEGVSVEGEKEHEPHSSHRSKEPTIHEEERTQDLRRTNSLMVPRGSGLVGALDEAETPLVQPQPELEVGKDAALTEQEAEGSSEQALLGDVQLDVGRGISQSEPDLSCMTANMDKATTESTSVTVAIPDVDPLVDSTVVNISNKTDGEASPLKEAETKEEEEEVEKKKKQKKEKRETGKAMVPHSSMFIFSTTNPIRRACHYIVNLRYFEMCILLVIAASSIALAAEDPVLTNSERNKVLRYFDYVFTGVFTFEMVIKMIDQGLILQDGSYFRDLWNILDFVVVVGALVAFALANALGTNKGRDIKTIKSLRVLRVLRPLKTIKRLPKLKAVFDCVVTSLKNVFNILIVYKLFMFIFAVIAVQLFKGKFFYCTDSSKDTEKECIGNYVDHEKNKMEVKGREWKRHEFHYDNIIWALLTLFTVSTGEGWPQVLQHSVDVTEEDRGPSRSNRMEMSIFYVVYFVVFPFFFVNIFVALIIITFQEQGDKMMEECSLEKNERACIDFAISAKPLTRYMPQNRHTFQYRVWHFVVSPSFEYTIMAMIALNTVVLMMKYYSAPCTYELALKYLNIAFTMVFSLECVLKVIAFGFLNYFRDTWNIFDFITVIGSITEIILTDSKLVNTSGFNMSFLKLFRAARLIKLLRQGYTIRILLWTFVQSFKALPYVCLLIAMLFFIYAIIGMQVFGNIKLDEESHINRHNNFRSFFGSLMLLFRSATGEAWQEIMLSCLGEKGCEPDTTAPSGQNESERCGTDLAYVYFVSFIFFCSFLMLNLFVAVIMDNFEYLTRDSSILGPHHLDEFVRVWAEYDRAACGRIHYTEMYEMLTLMSPPLGLGKRCPSKVAYKRLVLMNMPVAEDMTVHFTSTLMALIRTALDIKIAKGGADRQQLDSELQKETLAIWPHLSQKMLDLLVPMPKASDLTVGKIYAAMMIMDYYKQSKVKKQRQQLEEQVSTIESTLDGS

Target ORF information:

RefSeq Version	XM_017312855.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus calcium channel, voltage-dependent, R type, alpha 1E subunit (Cacna1e), transcript variant X18, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017312855.1

ORF Insert Sequence:

ATGGTGCCCG AGATGCGTGG ACTGAGCCCA GGCCGAAGGC TGCCCGAGCC CACCCTGTGC 
CCGGCTACAG GAGGGAGCCC TGAGCCCGGG GCTGCTGGCA GGCGCCGCCA CGTCCTGGCT 
CTGCCTGATG AGGAGGCATG GCGGCCGCAG CAGTGCGCCC TGCTCGAGGC CAATGCCTCC 
GACGAGGTGG GCATGTTGCC TGCTTCCCCG CGCTCCGCGG TGGCCGGCTT CGACAACTTC 
TTCCCGGTGC AGGAAGGCGA GGGCCCGGGC TGGGAGGGCA GCACCACGCT GGATGCAGGC 
TCCAGCCCCT TCCTTCCTGT GAGCCCAGAG GTCATGAAGC GGAGGCGAGG GGGGCTCATC 
GAGCAGCGGG ACATCATCAA GGCCCACGAG GCACACAAGA TGCAGAGCAC CCCGCAGGCC 
CGGCGCAAGG AGTGGGAGAT GGCTCGCTTC GGGGAGGCGG TGGTCGTTGG CAGGCCAGGC 
TCAGGCGATG GAGACTCGGA CCAGAGCAGG AACCGACAAG GAACCCCCGT CCCGGCCTCG 
GGGCCGGCGG CCGCCTACAA GCAGTCAAAA GCGCAGAGGG CGCGGACTAT GGCTTTGTAC 
AACCCCATAC CAGTCCGGCA GAACTGTTTC ACGGTCAACA GATCCCTGTT CATCTTCGGA 
GAAGATAACA TTGTCAGGAA ATACGCCAAG AAGCTCATCG ATTGGCCGCC ATTTGAGTAC 
ATGATCCTGG CCACCATCAT TGCCAACTGC ATCGTCTTGG CCCTGGAGCA GCATCTTCCT 
GAGGATGACA AGACCCCAAT GTCTCGAAGA CTGGAGAAGA CAGAACCATA TTTCATTGGG 
ATCTTCTGCT TTGAAGCTGG GATCAAAATT GTGGCTCTAG GGTTCATCTT CCATAAGGGT 
TCATACCTCC GTAATGGCTG GAATGTCATG GACTTCATCG TGGTCCTTAG TGGCATCCTG 
GCCACTGCGG GAACCCACTT CAACACCCAC GTGGACCTGC GGACCCTCCG GGCTGTGCGT 
GTCCTGAGGC CTTTAAAGCT GGTATCAGGA ATACCTAGCC TGCAGATCGT GCTGAAGTCT 
ATCATGAAGG CCATGGTGCC TCTTCTGCAG ATTGGCCTTC TGCTCTTCTT TGCCATCCTC 
ATGTTTGCTA TCATTGGCTT GGAGTTCTAC AGTGGCAAGT TGCATCGAGC ATGCTTCATG 
AACAATTCAG GTATTCTAGA GGGGTTTGAT CCTCCTCACC CGTGTGGTGT GCAAGGCTGC 
CCGGCTGGTT ATGAATGTAA GGACTGGATC GGCCCCAATG ACGGGATCAC CCAGTTTGAC 
AACATCCTTT TTGCCGTGCT GACTGTCTTC CAATGCATCA CTATGGAAGG GTGGACCACT 
GTGCTGTACA ATACCAATGA TGCCTTAGGA GCCACCTGGA ACTGGCTGTA CTTCATCCCT 
CTCATCATCA TCGGATCCTT CTTTGTTCTC AACCTCGTCC TGGGAGTGCT TTCCGGGGAA 
TTTGCCAAAG AAAGAGAGAG AGTGGAGAAC CGAAGAGCGT TCATGAAGCT CCGGCGCCAG 
CAACAGATTG AGCGAGAATT GAACGGCTAC CGTGCCTGGA TAGACAAAGC AGAGGAAGTC 
ATGCTTGCTG AAGAAAATAA AAACTCGGGA ACATCAGCCT TGGAAGTGCT TCGAAGGGCA 
ACCATCAAAA GGAGCCGGAC GGAGGCCATG ACCCGAGACT CCAGCGATGA GCACTGCGTT 
GATATCTCTT CAGTGGGCAC ACCTCTTGCC AGAGCCAGTA TCAAGAGCAC AAAAGTAGAT 
GGGGCCTCCT ATTTCCGGCA CAAGGAACGG CTTCTGCGCA TCTCTATCCG CCACATGGTC 
AAGTCCCAAG TCTTTTACTG GATCGTCCTG AGTGTTGTGG CTCTTAATAC TGCCTGTGTG 
GCCATTGTTC ATCACAACCA GCCACAGTGG CTCACTCACC TCCTCTACTA TGCAGAGTTT 
TTGTTTCTGG GACTCTTCCT GTTGGAGATG TCCCTGAAGA TGTATGGCAT GGGGCCACGC 
CTCTATTTTC ACTCATCCTT CAACTGCTTT GATTTTGGGG TCACAGTGGG CAGCATCTTT 
GAGGTTGTCT GGGCAATCTT CAGACCTGGC ACATCTTTTG GAATCAGTGT CTTACGAGCT 
CTCCGGCTCC TAAGGATATT TAAAATAACC AAGTATTGGG CATCCCTCCG GAATCTGGTT 
GTCTCCTTGA TGAGCTCCAT GAAGTCTATC ATCAGCTTAC TCTTCCTTCT CTTCCTCTTC 
ATCGTTGTCT TTGCTCTCCT GGGAATGCAG CTCTTTGGAG GCAGGTTTAA CTTTAATGAT 
GGGACTCCTT CAGCTAATTT TGACACCTTC CCTGCAGCTA TCATGACAGT GTTCCAGATC 
CTGACAGGGG AAGACTGGAA TGAGGTGATG TACAATGGCA TTCGCTCCCA GGGTGGGGTC 
AGCTCAGGCA TGTGGTCTGC CATCTACTTC ATTGTGCTCA CCTTGTTTGG AAACTACACG 
CTGCTAAATG TATTCTTGGC CATCGCTGTG GACAATCTGG CCAATGCCCA GGAACTGACC 
AAGGATGAGC AGGAGGAAGA AGAAGCCTTC AACCAGAAAC ATGCACTGCA GAAGGCCAAG 
GAGGTCAGCC CGATGTCTGC TCCCAACATG CCTTCCATTG AAAGAGACAG AAGGAGAAGA 
CACCACATGT CGATGTGGGA GCCACGCAGC AGCCACCTGA GGGAGCGGAG GCGCCGGCAC 
CACATGTCTG TGTGGGAACA ACGCACCAGC CAGCTGAGGA GGCACATGCA AATGTCTAGC 
CAAGAAGCCC TCAACAAAGA GGAGGCCCCG CCAATGAACC CCCTCAATCC ACTCAACCCA 
CTGAGTCCTC TCAACCCCCT CAATGCTCAC CCCAGCCTTT ATCGGAGGCC CAGGCCCATT 
GAGGGCCTAG CCCTAGGTCT GGGTCTTGAA AAGTGTGAAG AAGAACGCAT CAGTCGTGGG 
GGCTCCCTCA AGGGGGACAT AGGGGGCCTG ACTAGCGCCC TGGACAACCA GAGGAGCCCC 
TTGTCCTTGG GCAAACGGGA GCCACCGTGG CTGCCTAGAT CCTGTCATGG AAACTGTGAC 
CCAATCCAGC AGGAGGCTGG GGGAGGAGAG ACTGTGGTGA CTTTTGAGGA CCGGGCCAGG 
CACAGGCAGA GTCAGAGGCG AAGCCGGCAT CGCCGAGTCA GGACTGAGGG CAAGGACTCT 
GCCTCTGCAT CCCGAAGCAG GTCTGCCAGC CAGGAGCGGA GTCTGGATGA AGGGGTGTCC 
GTTGAGGGGG AGAAGGAACA TGAGCCTCAC AGCAGCCACA GGAGCAAAGA GCCGACCATC 
CACGAAGAGG AGAGAACCCA GGACTTAAGG AGGACCAACA GTCTGATGGT CCCCAGGGGC 
TCTGGACTGG TAGGGGCTCT TGATGAAGCA GAAACACCTC TAGTCCAGCC CCAACCTGAG 
CTGGAAGTGG GGAAGGATGC AGCTCTGACA GAGCAGGAGG CTGAAGGCAG CAGTGAACAA 
GCCCTTCTTG GGGACGTACA GCTGGATGTG GGCCGGGGCA TTAGCCAGAG TGAACCGGAT 
CTCTCCTGCA TGACAGCCAA CATGGACAAG GCTACCACGG AGAGCACCAG TGTCACGGTC 
GCCATCCCTG ATGTGGACCC CTTGGTGGAC TCGACAGTGG TGAACATTAG CAACAAGACG 
GATGGAGAAG CCAGTCCCTT GAAGGAGGCA GAGACTAAAG AGGAAGAAGA AGAGGTAGAG 
AAGAAGAAGA AGCAGAAGAA GGAGAAGCGA GAGACTGGAA AAGCCATGGT GCCCCATAGC 
TCTATGTTCA TCTTCAGCAC CACTAACCCG ATCCGCAGGG CCTGCCACTA CATTGTGAAC 
CTACGCTACT TTGAAATGTG CATTCTCTTG GTGATTGCGG CCAGCAGCAT TGCCCTGGCA 
GCCGAGGACC CTGTACTAAC TAACTCGGAG CGTAACAAAG TCCTGAGATA TTTCGACTAT 
GTTTTCACAG GAGTGTTTAC CTTTGAGATG GTTATAAAGA TGATAGACCA AGGCTTGATC 
CTGCAGGATG GATCATACTT CAGAGACCTC TGGAACATCC TGGACTTTGT AGTAGTGGTT 
GGTGCATTGG TAGCATTTGC TCTGGCGAAC GCTTTGGGAA CCAACAAGGG GAGGGATATC 
AAGACTATCA AGTCTCTGAG GGTACTTCGA GTTCTGCGGC CACTGAAAAC CATCAAGCGC 
TTGCCCAAGC TGAAGGCTGT GTTTGACTGC GTGGTGACCT CCTTGAAGAA TGTCTTCAAC 
ATACTCATTG TCTACAAGCT CTTCATGTTC ATCTTTGCCG TCATCGCTGT TCAGCTATTC 
AAAGGAAAGT TCTTTTATTG CACTGACAGT TCCAAGGACA CAGAGAAGGA GTGCATAGGC 
AACTATGTAG ACCACGAGAA AAACAAGATG GAGGTGAAGG GCCGGGAATG GAAGCGTCAT 
GAATTCCACT ATGACAACAT CATCTGGGCC CTGCTTACCC TCTTCACAGT CTCTACAGGG 
GAAGGATGGC CTCAGGTTCT GCAGCACTCT GTAGATGTGA CAGAGGAGGA CAGAGGCCCA 
AGTCGCAGCA ACCGCATGGA GATGTCCATC TTTTATGTGG TCTACTTTGT GGTCTTCCCT 
TTCTTCTTTG TCAATATCTT CGTGGCTCTC ATTATCATCA CCTTCCAGGA GCAAGGGGAC 
AAGATGATGG AGGAGTGTAG CCTGGAGAAG AATGAGCGGG CATGCATCGA CTTCGCCATT 
AGTGCCAAAC CTCTCACCCG CTACATGCCA CAGAACAGGC ACACCTTCCA GTACCGTGTG 
TGGCACTTTG TAGTCTCCCC ATCCTTTGAG TATACCATCA TGGCCATGAT TGCCTTGAAC 
ACTGTCGTGC TGATGATGAA GTACTACTCT GCTCCTTGCA CCTATGAGCT CGCCTTAAAG 
TACCTGAACA TCGCCTTCAC TATGGTGTTT TCCCTGGAGT GTGTCCTAAA GGTCATTGCT 
TTCGGCTTCC TGAACTATTT CCGAGATACC TGGAACATCT TTGACTTCAT CACGGTGATC 
GGCAGTATCA CAGAAATTAT CCTGACAGAT AGCAAGCTGG TGAACACCAG TGGCTTCAAT 
ATGAGCTTTC TGAAGCTCTT CCGAGCTGCA CGGCTCATAA AGCTTCTGAG ACAGGGCTAC 
ACCATCCGCA TCTTACTGTG GACCTTCGTG CAGTCCTTTA AGGCTCTTCC CTACGTCTGC 
CTTCTCATTG CCATGCTTTT CTTCATCTAC GCCATCATTG GGATGCAGGT GTTTGGAAAC 
ATAAAGTTAG ATGAGGAGAG TCACATCAAC CGGCACAACA ACTTCCGGAG TTTCTTTGGA 
TCCCTCATGC TGCTTTTCAG GAGTGCCACG GGGGAGGCTT GGCAGGAGAT TATGCTGTCC 
TGTCTCGGTG AGAAGGGTTG TGAGCCTGAC ACCACTGCAC CCTCAGGGCA GAATGAGAGT 
GAGCGCTGTG GCACTGACCT GGCCTACGTT TATTTTGTCT CCTTCATCTT CTTCTGCTCC 
TTCCTGATGC TCAACCTGTT CGTGGCTGTC ATCATGGACA ACTTTGAGTA CCTGACTCGA 
GATTCCTCCA TCCTGGGGCC TCACCACTTA GATGAGTTTG TCCGTGTCTG GGCAGAATAT 
GACAGAGCAG CATGTGGCCG CATCCATTAC ACTGAGATGT ATGAAATGCT GACTCTCATG 
TCACCACCGC TAGGCCTCGG CAAGAGATGT CCCTCCAAAG TGGCCTATAA GAGGCTGGTC 
CTGATGAACA TGCCAGTGGC TGAGGACATG ACAGTCCACT TCACCTCCAC ACTTATGGCT 
CTGATCCGGA CAGCTCTGGA CATTAAAATC GCCAAAGGTG GTGCAGACAG ACAGCAGCTA 
GACTCGGAGC TGCAAAAAGA GACCCTGGCC ATTTGGCCTC ACCTGTCCCA AAAGATGTTG 
GATCTGCTTG TGCCCATGCC CAAAGCCTCT GACCTGACTG TGGGCAAGAT CTATGCAGCA 
ATGATGATCA TGGACTACTA TAAACAGAGT AAGGTAAAGA AACAGAGACA GCAGCTGGAA 
GAACAGGTAA GCACCATTGA AAGCACATTG GATGGGTCAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cacna1e ( XM_030244154.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030100014.1 Mus musculus calcium channel, voltage-dependent, R type, alpha 1E subunit (Cacna1e), transcript variant X19, mRNA.

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CloneID	OMu84991
Clone ID Related Accession (Same CDS sequence)	XM_030244154.1
Accession Version	XM_030244154.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6816bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	voltage-dependent R-type calcium channel subunit alpha-1E isoform X15
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTCGCT TCGGGGAGGC GGTGGTCGTT GGCAGGCCAG GCTCAGGCGA TGGAGACTCG 
GACCAGAGCA GGAACCGACA AGGAACCCCC GTCCCGGCCT CGGGGCCGGC GGCCGCCTAC 
AAGCAGTCAA AAGCGCAGAG GGCGCGGACT ATGGCTTTGT ACAACCCCAT ACCAGTCCGG 
CAGAACTGTT TCACGGTCAA CAGATCCCTG TTCATCTTCG GAGAAGATAA CATTGTCAGG 
AAATACGCCA AGAAGCTCAT CGATTGGCCG CCATTTGAGT ACATGATCCT GGCCACCATC 
ATTGCCAACT GCATCGTCTT GGCCCTGGAG CAGCATCTTC CTGAGGATGA CAAGACCCCA 
ATGTCTCGAA GACTGGAGAA GACAGAACCA TATTTCATTG GGATCTTCTG CTTTGAAGCT 
GGGATCAAAA TTGTGGCTCT AGGGTTCATC TTCCATAAGG GTTCATACCT CCGTAATGGC 
TGGAATGTCA TGGACTTCAT CGTGGTCCTT AGTGGCATCC TGGCCACTGC GGGAACCCAC 
TTCAACACCC ACGTGGACCT GCGGACCCTC CGGGCTGTGC GTGTCCTGAG GCCTTTAAAG 
CTGGTATCAG GAATACCTAG CCTGCAGATC GTGCTGAAGT CTATCATGAA GGCCATGGTG 
CCTCTTCTGC AGATTGGCCT TCTGCTCTTC TTTGCCATCC TCATGTTTGC TATCATTGGC 
TTGGAGTTCT ACAGTGGCAA GTTGCATCGA GCATGCTTCA TGAACAATTC AGGTATTCTA 
GAGGGGTTTG ATCCTCCTCA CCCGTGTGGT GTGCAAGGCT GCCCGGCTGG TTATGAATGT 
AAGGACTGGA TCGGCCCCAA TGACGGGATC ACCCAGTTTG ACAACATCCT TTTTGCCGTG 
CTGACTGTCT TCCAATGCAT CACTATGGAA GGGTGGACCA CTGTGCTGTA CAATACCAAT 
GATGCCTTAG GAGCCACCTG GAACTGGCTG TACTTCATCC CTCTCATCAT CATCGGATCC 
TTCTTTGTTC TCAACCTCGT CCTGGGAGTG CTTTCCGGGG AATTTGCCAA AGAAAGAGAG 
AGAGTGGAGA ACCGAAGAGC GTTCATGAAG CTCCGGCGCC AGCAACAGAT TGAGCGAGAA 
TTGAACGGCT ACCGTGCCTG GATAGACAAA GCAGAGGAAG TCATGCTTGC TGAAGAAAAT 
AAAAACTCGG GAACATCAGC CTTGGAAGGC ACACCTCTTG CCAGAGCCAG TATCAAGAGC 
ACAAAAGTAG ATGGGGCCTC CTATTTCCGG CACAAGGAAC GGCTTCTGCG CATCTCTATC 
CGCCACATGG TCAAGTCCCA AGTCTTTTAC TGGATCGTCC TGAGTGTTGT GGCTCTTAAT 
ACTGCCTGTG TGGCCATTGT TCATCACAAC CAGCCACAGT GGCTCACTCA CCTCCTCTAC 
TATGCAGAGT TTTTGTTTCT GGGACTCTTC CTGTTGGAGA TGTCCCTGAA GATGTATGGC 
ATGGGGCCAC GCCTCTATTT TCACTCATCC TTCAACTGCT TTGATTTTGG GGTCACAGTG 
GGCAGCATCT TTGAGGTTGT CTGGGCAATC TTCAGACCTG GCACATCTTT TGGAATCAGT 
GTCTTACGAG CTCTCCGGCT CCTAAGGATA TTTAAAATAA CCAAGTATTG GGCATCCCTC 
CGGAATCTGG TTGTCTCCTT GATGAGCTCC ATGAAGTCTA TCATCAGCTT ACTCTTCCTT 
CTCTTCCTCT TCATCGTTGT CTTTGCTCTC CTGGGAATGC AGCTCTTTGG AGGCAGGTTT 
AACTTTAATG ATGGGACTCC TTCAGCTAAT TTTGACACCT TCCCTGCAGC TATCATGACA 
GTGTTCCAGA TCCTGACAGG GGAAGACTGG AATGAGGTGA TGTACAATGG CATTCGCTCC 
CAGGGTGGGG TCAGCTCAGG CATGTGGTCT GCCATCTACT TCATTGTGCT CACCTTGTTT 
GGAAACTACA CGCTGCTAAA TGTATTCTTG GCCATCGCTG TGGACAATCT GGCCAATGCC 
CAGGAACTGA CCAAGGATGA GCAGGAGGAA GAAGAAGCCT TCAACCAGAA ACATGCACTG 
CAGAAGGCCA AGGAGGTCAG CCCGATGTCT GCTCCCAACA TGCCTTCCAT TGAGAGGGAG 
CGGAGGCGCC GGCACCACAT GTCTGTGTGG GAACAACGCA CCAGCCAGCT GAGGAGGCAC 
ATGCAAATGT CTAGCCAAGA AGCCCTCAAC AAAGAGGAGG CCCCGCCAAT GAACCCCCTC 
AATCCACTCA ACCCACTGAG TCCTCTCAAC CCCCTCAATG CTCACCCCAG CCTTTATCGG 
AGGCCCAGGC CCATTGAGGG CCTAGCCCTA GGTCTGGGTC TTGAAAAGTG TGAAGAAGAA 
CGCATCAGTC GTGGGGGCTC CCTCAAGGGG GACATAGGGG GCCTGACTAG CGCCCTGGAC 
AACCAGAGGA GCCCCTTGTC CTTGGGCAAA CGGGAGCCAC CGTGGCTGCC TAGATCCTGT 
CATGGAAACT GTGACCCAAT CCAGCAGGAG GCTGGGGGAG GAGAGACTGT GGTGACTTTT 
GAGGACCGGG CCAGGCACAG GCAGAGTCAG AGGCGAAGCC GGCATCGCCG AGTCAGGACT 
GAGGGCAAGG ACTCTGCCTC TGCATCCCGA AGCAGGTCTG CCAGCCAGGA GCGGAGTCTG 
GATGAAGGGG TGTCCGTTGA GGGGGAGAAG GAACATGAGC CTCACAGCAG CCACAGGAGC 
AAAGAGCCGA CCATCCACGA AGAGGAGAGA ACCCAGGACT TAAGGAGGAC CAACAGTCTG 
ATGGTCCCCA GGGGCTCTGG ACTGGTAGGG GCTCTTGATG AAGCAGAAAC ACCTCTAGTC 
CAGCCCCAAC CTGAGCTGGA AGTGGGGAAG GATGCAGCTC TGACAGAGCA GGAGGCTGAA 
GGCAGCAGTG AACAAGCCCT TCTTGGGGAC GTACAGCTGG ATGTGGGCCG GGGCATTAGC 
CAGAGTGAAC CGGATCTCTC CTGCATGACA GCCAACATGG ACAAGGCTAC CACGGAGAGC 
ACCAGTGTCA CGGTCGCCAT CCCTGATGTG GACCCCTTGG TGGACTCGAC AGTGGTGAAC 
ATTAGCAACA AGACGGATGG AGAAGCCAGT CCCTTGAAGG AGGCAGAGAC TAAAGAGGAA 
GAAGAAGAGG TAGAGAAGAA GAAGAAGCAG AAGAAGGAGA AGCGAGAGAC TGGAAAAGCC 
ATGGTGCCCC ATAGCTCTAT GTTCATCTTC AGCACCACTA ACCCGATCCG CAGGGCCTGC 
CACTACATTG TGAACCTACG CTACTTTGAA ATGTGCATTC TCTTGGTGAT TGCGGCCAGC 
AGCATTGCCC TGGCAGCCGA GGACCCTGTA CTAACTAACT CGGAGCGTAA CAAAGTCCTG 
AGATATTTCG ACTATGTTTT CACAGGAGTG TTTACCTTTG AGATGGTTAT AAAGATGATA 
GACCAAGGCT TGATCCTGCA GGATGGATCA TACTTCAGAG ACCTCTGGAA CATCCTGGAC 
TTTGTAGTAG TGGTTGGTGC ATTGGTAGCA TTTGCTCTGG CGAACGCTTT GGGAACCAAC 
AAGGGGAGGG ATATCAAGAC TATCAAGTCT CTGAGGGTAC TTCGAGTTCT GCGGCCACTG 
AAAACCATCA AGCGCTTGCC CAAGCTGAAG GCTGTGTTTG ACTGCGTGGT GACCTCCTTG 
AAGAATGTCT TCAACATACT CATTGTCTAC AAGCTCTTCA TGTTCATCTT TGCCGTCATC 
GCTGTTCAGC TATTCAAAGG AAAGTTCTTT TATTGCACTG ACAGTTCCAA GGACACAGAG 
AAGGAGTGCA TAGGCAACTA TGTAGACCAC GAGAAAAACA AGATGGAGGT GAAGGGCCGG 
GAATGGAAGC GTCATGAATT CCACTATGAC AACATCATCT GGGCCCTGCT TACCCTCTTC 
ACAGTCTCTA CAGGGGAAGG ATGGCCTCAG GTTCTGCAGC ACTCTGTAGA TGTGACAGAG 
GAGGACAGAG GCCCAAGTCG CAGCAACCGC ATGGAGATGT CCATCTTTTA TGTGGTCTAC 
TTTGTGGTCT TCCCTTTCTT CTTTGTCAAT ATCTTCGTGG CTCTCATTAT CATCACCTTC 
CAGGAGCAAG GGGACAAGAT GATGGAGGAG TGTAGCCTGG AGAAGAATGA GCGGGCATGC 
ATCGACTTCG CCATTAGTGC CAAACCTCTC ACCCGCTACA TGCCACAGAA CAGGCACACC 
TTCCAGTACC GTGTGTGGCA CTTTGTAGTC TCCCCATCCT TTGAGTATAC CATCATGGCC 
ATGATTGCCT TGAACACTGT CGTGCTGATG ATGAAGTACT ACTCTGCTCC TTGCACCTAT 
GAGCTCGCCT TAAAGTACCT GAACATCGCC TTCACTATGG TGTTTTCCCT GGAGTGTGTC 
CTAAAGGTCA TTGCTTTCGG CTTCCTGAAC TATTTCCGAG ATACCTGGAA CATCTTTGAC 
TTCATCACGG TGATCGGCAG TATCACAGAA ATTATCCTGA CAGATAGCAA GCTGGTGAAC 
ACCAGTGGCT TCAATATGAG CTTTCTGAAG CTCTTCCGAG CTGCACGGCT CATAAAGCTT 
CTGAGACAGG GCTACACCAT CCGCATCTTA CTGTGGACCT TCGTGCAGTC CTTTAAGGCT 
CTTCCCTACG TCTGCCTTCT CATTGCCATG CTTTTCTTCA TCTACGCCAT CATTGGGATG 
CAGGTGTTTG GAAACATAAA GTTAGATGAG GAGAGTCACA TCAACCGGCA CAACAACTTC 
CGGAGTTTCT TTGGATCCCT CATGCTGCTT TTCAGGAGTG CCACGGGGGA GGCTTGGCAG 
GAGATTATGC TGTCCTGTCT CGGTGAGAAG GGTTGTGAGC CTGACACCAC TGCACCCTCA 
GGGCAGAATG AGAGTGAGCG CTGTGGCACT GACCTGGCCT ACGTTTATTT TGTCTCCTTC 
ATCTTCTTCT GCTCCTTCCT GATGCTCAAC CTGTTCGTGG CTGTCATCAT GGACAACTTT 
GAGTACCTGA CTCGAGATTC CTCCATCCTG GGGCCTCACC ACTTAGATGA GTTTGTCCGT 
GTCTGGGCAG AATATGACAG AGCAGCATGT GGCCGCATCC ATTACACTGA GATGTATGAA 
ATGCTGACTC TCATGTCACC ACCGCTAGGC CTCGGCAAGA GATGTCCCTC CAAAGTGGCC 
TATAAGAGGC TGGTCCTGAT GAACATGCCA GTGGCTGAGG ACATGACAGT CCACTTCACC 
TCCACACTTA TGGCTCTGAT CCGGACAGCT CTGGACATTA AAATCGCCAA AGGTGGTGCA 
GACAGACAGC AGCTAGACTC GGAGCTGCAA AAAGAGACCC TGGCCATTTG GCCTCACCTG 
TCCCAAAAGA TGTTGGATCT GCTTGTGCCC ATGCCCAAAG CCTCTGACCT GACTGTGGGC 
AAGATCTATG CAGCAATGAT GATCATGGAC TACTATAAAC AGAGTAAGGT AAAGAAACAG 
AGACAGCAGC TGGAAGAACA GAAAAATGCA CCCATGTTCC AGCGCATGGA GCCCTCATCA 
CTGCCTCAGG AGATCATTGC TAATGCCAAA GCCCTGCCTT ATCTCCAGCA GGACCCTGTT 
TCTGGCCTGA GTGGTCGGAG TGGATACCCT TCAATGAGTC CACTCTCCCC TCAAGAAATA 
TTCCAGTTGG CTTGTATGGA CCCAGCGGAT GATGGACAAT TCCAGGAACA ACAATCTCTG 
GTGAATGCAC AAGAGCCAGA GGTTAGTGAA TTAAAAAGTG TGCAGTCCTC TAACCATGGC 
ATCTACCTTC CTCCGGACAC CCAGGAGCAT GCGGGATCTG GGAGGGCATC CTCTATGCCA 
CGTCTGACTA TGGATCCCCA GGTGGTAACA GACCCTAGCT CTATGCGACG TTCATTTTCT 
ACAATTCGGG ATAAGCGTTC AAATTCCTCC TGGTTGGAGG AGTTCTCCAT GGAACGCAGC 
AGTGAAAACA CTTACAAGTC GCGGCGCAGA AGTTACCATT CCTCCCTGAG GCTGTCAGCA 
CACCGCCTGA ATTCTGACTC TGGTCACAAG TCTGACACTC ACCGATCAGG AGGCAGGGAG 
AGAGGCCGAT CCAAAGAACG AAAGCACCTC CTCTCTCCAG ATGTCTCCCG CTGCAATTCT 
GAAGAGCGAG GGACCCAGGC CGACTGGGAG TCCCCAGAAC GTCGCCAGTC CAGGTCACCC 
AGTGAAGGAA GGTCACAGAC CCCCAATAGA CAGGGTACCG GTTCCCTAAG TGAGAGCTCC 
ATCCCCTCTA TTTCCGACAC CAGCACCCCA AGACGAAGCC GTCGCCAGCT CCCACCTGTG 
CCACCAAAGC CTCGTCCCCT CCTCTCCTAC AGCTCCCTGA TGAGACACAC TGGCGGCATC 
TCTCCACCTC CTGATGGAAG TGAGGGTGGA TCCCCACTGG CCTCTCAAGC TCTGGAGAGC 
AACAGCGCTT GCCTGACCGA GTCTTCCAAC TCCTTGCACC CCCAGCAGGG CCAGCATCCT 
TCCCCACAGC ACTACATCTC CGAGCCCTAT CTGGCCCTCC ATGAAGACTC CCACGCCTCA 
GACTGTGGCG AGGAGGAGAC ACTCACCTTT GAGGCAGCCG TAGCCACTAG CTTGGGCCGG 
TCCAATACCA TTGGCTCTGC GCCACCCCTA CGGCACAGCT GGCAGATGCC TAATGGGCAC 
TATCGGCGGC GGAGGCGTGG GGGGCCTGGG CCTGGCATGA TGTGTGGAGC TGTCAGTGAC 
CTCCTGAGTG ACACGGAAGA AGACGATAAG TGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030100014.1
CDS	1744..8559
Translation	MARFGEAVVVGRPGSGDGDSDQSRNRQGTPVPASGPAAAYKQSKAQRARTMALYNPIPVRQNCFTVNRSLFIFGEDNIVRKYAKKLIDWPPFEYMILATIIANCIVLALEQHLPEDDKTPMSRRLEKTEPYFIGIFCFEAGIKIVALGFIFHKGSYLRNGWNVMDFIVVLSGILATAGTHFNTHVDLRTLRAVRVLRPLKLVSGIPSLQIVLKSIMKAMVPLLQIGLLLFFAILMFAIIGLEFYSGKLHRACFMNNSGILEGFDPPHPCGVQGCPAGYECKDWIGPNDGITQFDNILFAVLTVFQCITMEGWTTVLYNTNDALGATWNWLYFIPLIIIGSFFVLNLVLGVLSGEFAKERERVENRRAFMKLRRQQQIERELNGYRAWIDKAEEVMLAEENKNSGTSALEGTPLARASIKSTKVDGASYFRHKERLLRISIRHMVKSQVFYWIVLSVVALNTACVAIVHHNQPQWLTHLLYYAEFLFLGLFLLEMSLKMYGMGPRLYFHSSFNCFDFGVTVGSIFEVVWAIFRPGTSFGISVLRALRLLRIFKITKYWASLRNLVVSLMSSMKSIISLLFLLFLFIVVFALLGMQLFGGRFNFNDGTPSANFDTFPAAIMTVFQILTGEDWNEVMYNGIRSQGGVSSGMWSAIYFIVLTLFGNYTLLNVFLAIAVDNLANAQELTKDEQEEEEAFNQKHALQKAKEVSPMSAPNMPSIERERRRRHHMSVWEQRTSQLRRHMQMSSQEALNKEEAPPMNPLNPLNPLSPLNPLNAHPSLYRRPRPIEGLALGLGLEKCEEERISRGGSLKGDIGGLTSALDNQRSPLSLGKREPPWLPRSCHGNCDPIQQEAGGGETVVTFEDRARHRQSQRRSRHRRVRTEGKDSASASRSRSASQERSLDEGVSVEGEKEHEPHSSHRSKEPTIHEEERTQDLRRTNSLMVPRGSGLVGALDEAETPLVQPQPELEVGKDAALTEQEAEGSSEQALLGDVQLDVGRGISQSEPDLSCMTANMDKATTESTSVTVAIPDVDPLVDSTVVNISNKTDGEASPLKEAETKEEEEEVEKKKKQKKEKRETGKAMVPHSSMFIFSTTNPIRRACHYIVNLRYFEMCILLVIAASSIALAAEDPVLTNSERNKVLRYFDYVFTGVFTFEMVIKMIDQGLILQDGSYFRDLWNILDFVVVVGALVAFALANALGTNKGRDIKTIKSLRVLRVLRPLKTIKRLPKLKAVFDCVVTSLKNVFNILIVYKLFMFIFAVIAVQLFKGKFFYCTDSSKDTEKECIGNYVDHEKNKMEVKGREWKRHEFHYDNIIWALLTLFTVSTGEGWPQVLQHSVDVTEEDRGPSRSNRMEMSIFYVVYFVVFPFFFVNIFVALIIITFQEQGDKMMEECSLEKNERACIDFAISAKPLTRYMPQNRHTFQYRVWHFVVSPSFEYTIMAMIALNTVVLMMKYYSAPCTYELALKYLNIAFTMVFSLECVLKVIAFGFLNYFRDTWNIFDFITVIGSITEIILTDSKLVNTSGFNMSFLKLFRAARLIKLLRQGYTIRILLWTFVQSFKALPYVCLLIAMLFFIYAIIGMQVFGNIKLDEESHINRHNNFRSFFGSLMLLFRSATGEAWQEIMLSCLGEKGCEPDTTAPSGQNESERCGTDLAYVYFVSFIFFCSFLMLNLFVAVIMDNFEYLTRDSSILGPHHLDEFVRVWAEYDRAACGRIHYTEMYEMLTLMSPPLGLGKRCPSKVAYKRLVLMNMPVAEDMTVHFTSTLMALIRTALDIKIAKGGADRQQLDSELQKETLAIWPHLSQKMLDLLVPMPKASDLTVGKIYAAMMIMDYYKQSKVKKQRQQLEEQKNAPMFQRMEPSSLPQEIIANAKALPYLQQDPVSGLSGRSGYPSMSPLSPQEIFQLACMDPADDGQFQEQQSLVNAQEPEVSELKSVQSSNHGIYLPPDTQEHAGSGRASSMPRLTMDPQVVTDPSSMRRSFSTIRDKRSNSSWLEEFSMERSSENTYKSRRRSYHSSLRLSAHRLNSDSGHKSDTHRSGGRERGRSKERKHLLSPDVSRCNSEERGTQADWESPERRQSRSPSEGRSQTPNRQGTGSLSESSIPSISDTSTPRRSRRQLPPVPPKPRPLLSYSSLMRHTGGISPPPDGSEGGSPLASQALESNSACLTESSNSLHPQQGQHPSPQHYISEPYLALHEDSHASDCGEEETLTFEAAVATSLGRSNTIGSAPPLRHSWQMPNGHYRRRRRGGPGPGMMCGAVSDLLSDTEEDDKC

Target ORF information:

RefSeq Version	XM_030244154.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus calcium channel, voltage-dependent, R type, alpha 1E subunit (Cacna1e), transcript variant X19, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030244154.1

ORF Insert Sequence:

ATGGCTCGCT TCGGGGAGGC GGTGGTCGTT GGCAGGCCAG GCTCAGGCGA TGGAGACTCG 
GACCAGAGCA GGAACCGACA AGGAACCCCC GTCCCGGCCT CGGGGCCGGC GGCCGCCTAC 
AAGCAGTCAA AAGCGCAGAG GGCGCGGACT ATGGCTTTGT ACAACCCCAT ACCAGTCCGG 
CAGAACTGTT TCACGGTCAA CAGATCCCTG TTCATCTTCG GAGAAGATAA CATTGTCAGG 
AAATACGCCA AGAAGCTCAT CGATTGGCCG CCATTTGAGT ACATGATCCT GGCCACCATC 
ATTGCCAACT GCATCGTCTT GGCCCTGGAG CAGCATCTTC CTGAGGATGA CAAGACCCCA 
ATGTCTCGAA GACTGGAGAA GACAGAACCA TATTTCATTG GGATCTTCTG CTTTGAAGCT 
GGGATCAAAA TTGTGGCTCT AGGGTTCATC TTCCATAAGG GTTCATACCT CCGTAATGGC 
TGGAATGTCA TGGACTTCAT CGTGGTCCTT AGTGGCATCC TGGCCACTGC GGGAACCCAC 
TTCAACACCC ACGTGGACCT GCGGACCCTC CGGGCTGTGC GTGTCCTGAG GCCTTTAAAG 
CTGGTATCAG GAATACCTAG CCTGCAGATC GTGCTGAAGT CTATCATGAA GGCCATGGTG 
CCTCTTCTGC AGATTGGCCT TCTGCTCTTC TTTGCCATCC TCATGTTTGC TATCATTGGC 
TTGGAGTTCT ACAGTGGCAA GTTGCATCGA GCATGCTTCA TGAACAATTC AGGTATTCTA 
GAGGGGTTTG ATCCTCCTCA CCCGTGTGGT GTGCAAGGCT GCCCGGCTGG TTATGAATGT 
AAGGACTGGA TCGGCCCCAA TGACGGGATC ACCCAGTTTG ACAACATCCT TTTTGCCGTG 
CTGACTGTCT TCCAATGCAT CACTATGGAA GGGTGGACCA CTGTGCTGTA CAATACCAAT 
GATGCCTTAG GAGCCACCTG GAACTGGCTG TACTTCATCC CTCTCATCAT CATCGGATCC 
TTCTTTGTTC TCAACCTCGT CCTGGGAGTG CTTTCCGGGG AATTTGCCAA AGAAAGAGAG 
AGAGTGGAGA ACCGAAGAGC GTTCATGAAG CTCCGGCGCC AGCAACAGAT TGAGCGAGAA 
TTGAACGGCT ACCGTGCCTG GATAGACAAA GCAGAGGAAG TCATGCTTGC TGAAGAAAAT 
AAAAACTCGG GAACATCAGC CTTGGAAGGC ACACCTCTTG CCAGAGCCAG TATCAAGAGC 
ACAAAAGTAG ATGGGGCCTC CTATTTCCGG CACAAGGAAC GGCTTCTGCG CATCTCTATC 
CGCCACATGG TCAAGTCCCA AGTCTTTTAC TGGATCGTCC TGAGTGTTGT GGCTCTTAAT 
ACTGCCTGTG TGGCCATTGT TCATCACAAC CAGCCACAGT GGCTCACTCA CCTCCTCTAC 
TATGCAGAGT TTTTGTTTCT GGGACTCTTC CTGTTGGAGA TGTCCCTGAA GATGTATGGC 
ATGGGGCCAC GCCTCTATTT TCACTCATCC TTCAACTGCT TTGATTTTGG GGTCACAGTG 
GGCAGCATCT TTGAGGTTGT CTGGGCAATC TTCAGACCTG GCACATCTTT TGGAATCAGT 
GTCTTACGAG CTCTCCGGCT CCTAAGGATA TTTAAAATAA CCAAGTATTG GGCATCCCTC 
CGGAATCTGG TTGTCTCCTT GATGAGCTCC ATGAAGTCTA TCATCAGCTT ACTCTTCCTT 
CTCTTCCTCT TCATCGTTGT CTTTGCTCTC CTGGGAATGC AGCTCTTTGG AGGCAGGTTT 
AACTTTAATG ATGGGACTCC TTCAGCTAAT TTTGACACCT TCCCTGCAGC TATCATGACA 
GTGTTCCAGA TCCTGACAGG GGAAGACTGG AATGAGGTGA TGTACAATGG CATTCGCTCC 
CAGGGTGGGG TCAGCTCAGG CATGTGGTCT GCCATCTACT TCATTGTGCT CACCTTGTTT 
GGAAACTACA CGCTGCTAAA TGTATTCTTG GCCATCGCTG TGGACAATCT GGCCAATGCC 
CAGGAACTGA CCAAGGATGA GCAGGAGGAA GAAGAAGCCT TCAACCAGAA ACATGCACTG 
CAGAAGGCCA AGGAGGTCAG CCCGATGTCT GCTCCCAACA TGCCTTCCAT TGAGAGGGAG 
CGGAGGCGCC GGCACCACAT GTCTGTGTGG GAACAACGCA CCAGCCAGCT GAGGAGGCAC 
ATGCAAATGT CTAGCCAAGA AGCCCTCAAC AAAGAGGAGG CCCCGCCAAT GAACCCCCTC 
AATCCACTCA ACCCACTGAG TCCTCTCAAC CCCCTCAATG CTCACCCCAG CCTTTATCGG 
AGGCCCAGGC CCATTGAGGG CCTAGCCCTA GGTCTGGGTC TTGAAAAGTG TGAAGAAGAA 
CGCATCAGTC GTGGGGGCTC CCTCAAGGGG GACATAGGGG GCCTGACTAG CGCCCTGGAC 
AACCAGAGGA GCCCCTTGTC CTTGGGCAAA CGGGAGCCAC CGTGGCTGCC TAGATCCTGT 
CATGGAAACT GTGACCCAAT CCAGCAGGAG GCTGGGGGAG GAGAGACTGT GGTGACTTTT 
GAGGACCGGG CCAGGCACAG GCAGAGTCAG AGGCGAAGCC GGCATCGCCG AGTCAGGACT 
GAGGGCAAGG ACTCTGCCTC TGCATCCCGA AGCAGGTCTG CCAGCCAGGA GCGGAGTCTG 
GATGAAGGGG TGTCCGTTGA GGGGGAGAAG GAACATGAGC CTCACAGCAG CCACAGGAGC 
AAAGAGCCGA CCATCCACGA AGAGGAGAGA ACCCAGGACT TAAGGAGGAC CAACAGTCTG 
ATGGTCCCCA GGGGCTCTGG ACTGGTAGGG GCTCTTGATG AAGCAGAAAC ACCTCTAGTC 
CAGCCCCAAC CTGAGCTGGA AGTGGGGAAG GATGCAGCTC TGACAGAGCA GGAGGCTGAA 
GGCAGCAGTG AACAAGCCCT TCTTGGGGAC GTACAGCTGG ATGTGGGCCG GGGCATTAGC 
CAGAGTGAAC CGGATCTCTC CTGCATGACA GCCAACATGG ACAAGGCTAC CACGGAGAGC 
ACCAGTGTCA CGGTCGCCAT CCCTGATGTG GACCCCTTGG TGGACTCGAC AGTGGTGAAC 
ATTAGCAACA AGACGGATGG AGAAGCCAGT CCCTTGAAGG AGGCAGAGAC TAAAGAGGAA 
GAAGAAGAGG TAGAGAAGAA GAAGAAGCAG AAGAAGGAGA AGCGAGAGAC TGGAAAAGCC 
ATGGTGCCCC ATAGCTCTAT GTTCATCTTC AGCACCACTA ACCCGATCCG CAGGGCCTGC 
CACTACATTG TGAACCTACG CTACTTTGAA ATGTGCATTC TCTTGGTGAT TGCGGCCAGC 
AGCATTGCCC TGGCAGCCGA GGACCCTGTA CTAACTAACT CGGAGCGTAA CAAAGTCCTG 
AGATATTTCG ACTATGTTTT CACAGGAGTG TTTACCTTTG AGATGGTTAT AAAGATGATA 
GACCAAGGCT TGATCCTGCA GGATGGATCA TACTTCAGAG ACCTCTGGAA CATCCTGGAC 
TTTGTAGTAG TGGTTGGTGC ATTGGTAGCA TTTGCTCTGG CGAACGCTTT GGGAACCAAC 
AAGGGGAGGG ATATCAAGAC TATCAAGTCT CTGAGGGTAC TTCGAGTTCT GCGGCCACTG 
AAAACCATCA AGCGCTTGCC CAAGCTGAAG GCTGTGTTTG ACTGCGTGGT GACCTCCTTG 
AAGAATGTCT TCAACATACT CATTGTCTAC AAGCTCTTCA TGTTCATCTT TGCCGTCATC 
GCTGTTCAGC TATTCAAAGG AAAGTTCTTT TATTGCACTG ACAGTTCCAA GGACACAGAG 
AAGGAGTGCA TAGGCAACTA TGTAGACCAC GAGAAAAACA AGATGGAGGT GAAGGGCCGG 
GAATGGAAGC GTCATGAATT CCACTATGAC AACATCATCT GGGCCCTGCT TACCCTCTTC 
ACAGTCTCTA CAGGGGAAGG ATGGCCTCAG GTTCTGCAGC ACTCTGTAGA TGTGACAGAG 
GAGGACAGAG GCCCAAGTCG CAGCAACCGC ATGGAGATGT CCATCTTTTA TGTGGTCTAC 
TTTGTGGTCT TCCCTTTCTT CTTTGTCAAT ATCTTCGTGG CTCTCATTAT CATCACCTTC 
CAGGAGCAAG GGGACAAGAT GATGGAGGAG TGTAGCCTGG AGAAGAATGA GCGGGCATGC 
ATCGACTTCG CCATTAGTGC CAAACCTCTC ACCCGCTACA TGCCACAGAA CAGGCACACC 
TTCCAGTACC GTGTGTGGCA CTTTGTAGTC TCCCCATCCT TTGAGTATAC CATCATGGCC 
ATGATTGCCT TGAACACTGT CGTGCTGATG ATGAAGTACT ACTCTGCTCC TTGCACCTAT 
GAGCTCGCCT TAAAGTACCT GAACATCGCC TTCACTATGG TGTTTTCCCT GGAGTGTGTC 
CTAAAGGTCA TTGCTTTCGG CTTCCTGAAC TATTTCCGAG ATACCTGGAA CATCTTTGAC 
TTCATCACGG TGATCGGCAG TATCACAGAA ATTATCCTGA CAGATAGCAA GCTGGTGAAC 
ACCAGTGGCT TCAATATGAG CTTTCTGAAG CTCTTCCGAG CTGCACGGCT CATAAAGCTT 
CTGAGACAGG GCTACACCAT CCGCATCTTA CTGTGGACCT TCGTGCAGTC CTTTAAGGCT 
CTTCCCTACG TCTGCCTTCT CATTGCCATG CTTTTCTTCA TCTACGCCAT CATTGGGATG 
CAGGTGTTTG GAAACATAAA GTTAGATGAG GAGAGTCACA TCAACCGGCA CAACAACTTC 
CGGAGTTTCT TTGGATCCCT CATGCTGCTT TTCAGGAGTG CCACGGGGGA GGCTTGGCAG 
GAGATTATGC TGTCCTGTCT CGGTGAGAAG GGTTGTGAGC CTGACACCAC TGCACCCTCA 
GGGCAGAATG AGAGTGAGCG CTGTGGCACT GACCTGGCCT ACGTTTATTT TGTCTCCTTC 
ATCTTCTTCT GCTCCTTCCT GATGCTCAAC CTGTTCGTGG CTGTCATCAT GGACAACTTT 
GAGTACCTGA CTCGAGATTC CTCCATCCTG GGGCCTCACC ACTTAGATGA GTTTGTCCGT 
GTCTGGGCAG AATATGACAG AGCAGCATGT GGCCGCATCC ATTACACTGA GATGTATGAA 
ATGCTGACTC TCATGTCACC ACCGCTAGGC CTCGGCAAGA GATGTCCCTC CAAAGTGGCC 
TATAAGAGGC TGGTCCTGAT GAACATGCCA GTGGCTGAGG ACATGACAGT CCACTTCACC 
TCCACACTTA TGGCTCTGAT CCGGACAGCT CTGGACATTA AAATCGCCAA AGGTGGTGCA 
GACAGACAGC AGCTAGACTC GGAGCTGCAA AAAGAGACCC TGGCCATTTG GCCTCACCTG 
TCCCAAAAGA TGTTGGATCT GCTTGTGCCC ATGCCCAAAG CCTCTGACCT GACTGTGGGC 
AAGATCTATG CAGCAATGAT GATCATGGAC TACTATAAAC AGAGTAAGGT AAAGAAACAG 
AGACAGCAGC TGGAAGAACA GAAAAATGCA CCCATGTTCC AGCGCATGGA GCCCTCATCA 
CTGCCTCAGG AGATCATTGC TAATGCCAAA GCCCTGCCTT ATCTCCAGCA GGACCCTGTT 
TCTGGCCTGA GTGGTCGGAG TGGATACCCT TCAATGAGTC CACTCTCCCC TCAAGAAATA 
TTCCAGTTGG CTTGTATGGA CCCAGCGGAT GATGGACAAT TCCAGGAACA ACAATCTCTG 
GTGAATGCAC AAGAGCCAGA GGTTAGTGAA TTAAAAAGTG TGCAGTCCTC TAACCATGGC 
ATCTACCTTC CTCCGGACAC CCAGGAGCAT GCGGGATCTG GGAGGGCATC CTCTATGCCA 
CGTCTGACTA TGGATCCCCA GGTGGTAACA GACCCTAGCT CTATGCGACG TTCATTTTCT 
ACAATTCGGG ATAAGCGTTC AAATTCCTCC TGGTTGGAGG AGTTCTCCAT GGAACGCAGC 
AGTGAAAACA CTTACAAGTC GCGGCGCAGA AGTTACCATT CCTCCCTGAG GCTGTCAGCA 
CACCGCCTGA ATTCTGACTC TGGTCACAAG TCTGACACTC ACCGATCAGG AGGCAGGGAG 
AGAGGCCGAT CCAAAGAACG AAAGCACCTC CTCTCTCCAG ATGTCTCCCG CTGCAATTCT 
GAAGAGCGAG GGACCCAGGC CGACTGGGAG TCCCCAGAAC GTCGCCAGTC CAGGTCACCC 
AGTGAAGGAA GGTCACAGAC CCCCAATAGA CAGGGTACCG GTTCCCTAAG TGAGAGCTCC 
ATCCCCTCTA TTTCCGACAC CAGCACCCCA AGACGAAGCC GTCGCCAGCT CCCACCTGTG 
CCACCAAAGC CTCGTCCCCT CCTCTCCTAC AGCTCCCTGA TGAGACACAC TGGCGGCATC 
TCTCCACCTC CTGATGGAAG TGAGGGTGGA TCCCCACTGG CCTCTCAAGC TCTGGAGAGC 
AACAGCGCTT GCCTGACCGA GTCTTCCAAC TCCTTGCACC CCCAGCAGGG CCAGCATCCT 
TCCCCACAGC ACTACATCTC CGAGCCCTAT CTGGCCCTCC ATGAAGACTC CCACGCCTCA 
GACTGTGGCG AGGAGGAGAC ACTCACCTTT GAGGCAGCCG TAGCCACTAG CTTGGGCCGG 
TCCAATACCA TTGGCTCTGC GCCACCCCTA CGGCACAGCT GGCAGATGCC TAATGGGCAC 
TATCGGCGGC GGAGGCGTGG GGGGCCTGGG CCTGGCATGA TGTGTGGAGC TGTCAGTGAC 
CTCCTGAGTG ACACGGAAGA AGACGATAAG TGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cacna1e ( NM_009782.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_033912.2 Mus musculus calcium channel, voltage-dependent, R type, alpha 1E subunit (Cacna1e), mRNA.

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CloneID	OMu13081
Clone ID Related Accession (Same CDS sequence)	NM_009782.3
Accession Version	NM_009782.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6822bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-25
Organism	Mus musculus(house mouse)
Product	voltage-dependent R-type calcium channel subunit alpha-1E
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC101727.8, AC099707.14 and AC122040.4. On Nov 15, 2006 this sequence version replaced NM_009782.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC150750.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END##

ATGGCTCGCT TCGGGGAGGC GGTGGTCGTT GGCAGGCCAG GCTCAGGCGA TGGAGACTCG 
GACCAGAGCA GGAACCGACA AGGAACCCCC GTCCCGGCCT CGGGGCCGGC GGCCGCCTAC 
AAGCAGTCAA AAGCGCAGAG GGCGCGGACT ATGGCTTTGT ACAACCCCAT ACCAGTCCGG 
CAGAACTGTT TCACGGTCAA CAGATCCCTG TTCATCTTCG GAGAAGATAA CATTGTCAGG 
AAATACGCCA AGAAGCTCAT CGATTGGCCG CCATTTGAGT ACATGATCCT GGCCACCATC 
ATTGCCAACT GCATCGTCTT GGCCCTGGAG CAGCATCTTC CTGAGGATGA CAAGACCCCA 
ATGTCTCGAA GACTGGAGAA GACAGAACCA TATTTCATTG GGATCTTCTG CTTTGAAGCT 
GGGATCAAAA TTGTGGCTCT AGGGTTCATC TTCCATAAGG GTTCATACCT CCGTAATGGC 
TGGAATGTCA TGGACTTCAT CGTGGTCCTT AGTGGCATCC TGGCCACTGC GGGAACCCAC 
TTCAACACCC ACGTGGACCT GCGGACCCTC CGGGCTGTGC GTGTCCTGAG GCCTTTAAAG 
CTGGTATCAG GAATACCTAG CCTGCAGATC GTGCTGAAGT CTATCATGAA GGCCATGGTG 
CCTCTTCTGC AGATTGGCCT TCTGCTCTTC TTTGCCATCC TCATGTTTGC TATCATTGGC 
TTGGAGTTCT ACAGTGGCAA GTTGCATCGA GCATGCTTCA TGAACAATTC AGGTATTCTA 
GAGGGGTTTG ATCCTCCTCA CCCGTGTGGT GTGCAAGGCT GCCCGGCTGG TTATGAATGT 
AAGGACTGGA TCGGCCCCAA TGACGGGATC ACCCAGTTTG ACAACATCCT TTTTGCCGTG 
CTGACTGTCT TCCAATGCAT CACTATGGAA GGGTGGACCA CTGTGCTGTA CAATACCAAT 
GATGCCTTAG GAGCCACCTG GAACTGGCTG TACTTCATCC CTCTCATCAT CATCGGATCC 
TTCTTTGTTC TCAACCTCGT CCTGGGAGTG CTTTCCGGGG AATTTGCCAA AGAAAGAGAG 
AGAGTGGAGA ACCGAAGAGC GTTCATGAAG CTCCGGCGCC AGCAACAGAT TGAGCGAGAA 
TTGAACGGCT ACCGTGCCTG GATAGACAAA GCAGAGGAAG TCATGCTTGC TGAAGAAAAT 
AAAAACTCGG GAACATCAGC CTTGGAAGTG CTTCGAAGGG CAACCATCAA AAGGAGCCGG 
ACGGAGGCCA TGACCCGAGA CTCCAGCGAT GAGCACTGCG TTGATATCTC TTCAGTGGGC 
ACACCTCTTG CCAGAGCCAG TATCAAGAGC ACAAAAGTAG ATGGGGCCTC CTATTTCCGG 
CACAAGGAAC GGCTTCTGCG CATCTCTATC CGCCACATGG TCAAGTCCCA AGTCTTTTAC 
TGGATCGTCC TGAGTGTTGT GGCTCTTAAT ACTGCCTGTG TGGCCATTGT TCATCACAAC 
CAGCCACAGT GGCTCACTCA CCTCCTCTAC TATGCAGAGT TTTTGTTTCT GGGACTCTTC 
CTGTTGGAGA TGTCCCTGAA GATGTATGGC ATGGGGCCAC GCCTCTATTT TCACTCATCC 
TTCAACTGCT TTGATTTTGG GGTCACAGTG GGCAGCATCT TTGAGGTTGT CTGGGCAATC 
TTCAGACCTG GCACATCTTT TGGAATCAGT GTCTTACGAG CTCTCCGGCT CCTAAGGATA 
TTTAAAATAA CCAAGTATTG GGCATCCCTC CGGAATCTGG TTGTCTCCTT GATGAGCTCC 
ATGAAGTCTA TCATCAGCTT ACTCTTCCTT CTCTTCCTCT TCATCGTTGT CTTTGCTCTC 
CTGGGAATGC AGCTCTTTGG AGGCAGGTTT AACTTTAATG ATGGGACTCC TTCAGCTAAT 
TTTGACACCT TCCCTGCAGC TATCATGACA GTGTTCCAGA TCCTGACAGG GGAAGACTGG 
AATGAGGTGA TGTACAATGG CATTCGCTCC CAGGGTGGGG TCAGCTCAGG CATGTGGTCT 
GCCATCTACT TCATTGTGCT CACCTTGTTT GGAAACTACA CGCTGCTAAA TGTATTCTTG 
GCCATCGCTG TGGACAATCT GGCCAATGCC CAGGAACTGA CCAAGGATGA GCAGGAGGAA 
GAAGAAGCCT TCAACCAGAA ACATGCACTG CAGAAGGCCA AGGAGGTCAG CCCGATGTCT 
GCTCCCAACA TGCCTTCCAT TGAAAGAGAC AGAAGGAGAA GACACCACAT GTCGATGTGG 
GAGCCACGCA GCAGCCACCT GAGGGAGCGG AGGCGCCGGC ACCACATGTC TGTGTGGGAA 
CAACGCACCA GCCAGCTGAG GAGGCACATG CAAATGTCTA GCCAAGAAGC CCTCAACAAA 
GAGGAGGCCC CGCCAATGAA CCCCCTCAAT CCACTCAACC CACTGAGTCC TCTCAACCCC 
CTCAATGCTC ACCCCAGCCT TTATCGGAGG CCCAGGCCCA TTGAGGGCCT AGCCCTAGGT 
CTGGGTCTTG AAAAGTGTGA AGAAGAACGC ATCAGTCGTG GGGGCTCCCT CAAGGGGGAC 
ATAGGGGGCC TGACTAGCGC CCTGGACAAC CAGAGGAGCC CCTTGTCCTT GGGCAAACGG 
GAGCCACCGT GGCTGCCTAG ATCCTGTCAT GGAAACTGTG ACCCAATCCA GCAGGAGGCT 
GGGGGAGGAG AGACTGTGGT GACTTTTGAG GACCGGGCCA GGCACAGGCA GAGTCAGAGG 
CGAAGCCGGC ATCGCCGAGT CAGGACTGAG GGCAAGGACT CTGCCTCTGC ATCCCGAAGC 
AGGTCTGCCA GCCAGGAGCG GAGTCTGGAT GAAGGGGTGT CCGTTGAGGG GGAGAAGGAA 
CATGAGCCTC ACAGCAGCCA CAGGAGCAAA GAGCCGACCA TCCACGAAGA GGAGAGAACC 
CAGGACTTAA GGAGGACCAA CAGTCTGATG GTCCCCAGGG GCTCTGGACT GGTAGGGGCT 
CTTGATGAAG CAGAAACACC TCTAGTCCAG CCCCAACCTG AGCTGGAAGT GGGGAAGGAT 
GCAGCTCTGA CAGAGCAGGA GGCTGAAGGC AGCAGTGAAC AAGCCCTTCT TGGGGACGTA 
CAGCTGGATG TGGGCCGGGG CATTAGCCAG AGTGAACCGG ATCTCTCCTG CATGACAGCC 
AACATGGACA AGGCTACCAC GGAGAGCACC AGTGTCACGG TCGCCATCCC TGATGTGGAC 
CCCTTGGTGG ACTCGACAGT GGTGAACATT AGCAACAAGA CGGATGGAGA AGCCAGTCCC 
TTGAAGGAGG CAGAGACTAA AGAGGAAGAA GAAGAGGTAG AGAAGAAGAA GAAGCAGAAG 
AAGGAGAAGC GAGAGACTGG AAAAGCCATG GTGCCCCATA GCTCTATGTT CATCTTCAGC 
ACCACTAACC CGATCCGCAG GGCCTGCCAC TACATTGTGA ACCTACGCTA CTTTGAAATG 
TGCATTCTCT TGGTGATTGC GGCCAGCAGC ATTGCCCTGG CAGCCGAGGA CCCTGTACTA 
ACTAACTCGG AGCGTAACAA AGTCCTGAGA TATTTCGACT ATGTTTTCAC AGGAGTGTTT 
ACCTTTGAGA TGGTTATAAA GATGATAGAC CAAGGCTTGA TCCTGCAGGA TGGATCATAC 
TTCAGAGACC TCTGGAACAT CCTGGACTTT GTAGTAGTGG TTGGTGCATT GGTAGCATTT 
GCTCTGGCGA ACGCTTTGGG AACCAACAAG GGGAGGGATA TCAAGACTAT CAAGTCTCTG 
AGGGTACTTC GAGTTCTGCG GCCACTGAAA ACCATCAAGC GCTTGCCCAA GCTGAAGGCT 
GTGTTTGACT GCGTGGTGAC CTCCTTGAAG AATGTCTTCA ACATACTCAT TGTCTACAAG 
CTCTTCATGT TCATCTTTGC CGTCATCGCT GTTCAGCTAT TCAAAGGAAA GTTCTTTTAT 
TGCACTGACA GTTCCAAGGA CACAGAGAAG GAGTGCATAG GCAACTATGT AGACCACGAG 
AAAAACAAGA TGGAGGTGAA GGGCCGGGAA TGGAAGCGTC ATGAATTCCA CTATGACAAC 
ATCATCTGGG CCCTGCTTAC CCTCTTCACA GTCTCTACAG GGGAAGGATG GCCTCAGGTT 
CTGCAGCACT CTGTAGATGT GACAGAGGAG GACAGAGGCC CAAGTCGCAG CAACCGCATG 
GAGATGTCCA TCTTTTATGT GGTCTACTTT GTGGTCTTCC CTTTCTTCTT TGTCAATATC 
TTCGTGGCTC TCATTATCAT CACCTTCCAG GAGCAAGGGG ACAAGATGAT GGAGGAGTGT 
AGCCTGGAGA AGAATGAGCG GGCATGCATC GACTTCGCCA TTAGTGCCAA ACCTCTCACC 
CGCTACATGC CACAGAACAG GCACACCTTC CAGTACCGTG TGTGGCACTT TGTAGTCTCC 
CCATCCTTTG AGTATACCAT CATGGCCATG ATTGCCTTGA ACACTGTCGT GCTGATGATG 
AAGTACTACT CTGCTCCTTG CACCTATGAG CTCGCCTTAA AGTACCTGAA CATCGCCTTC 
ACTATGGTGT TTTCCCTGGA GTGTGTCCTA AAGGTCATTG CTTTCGGCTT CCTGAACTAT 
TTCCGAGATA CCTGGAACAT CTTTGACTTC ATCACGGTGA TCGGCAGTAT CACAGAAATT 
ATCCTGACAG ATAGCAAGCT GGTGAACACC AGTGGCTTCA ATATGAGCTT TCTGAAGCTC 
TTCCGAGCTG CACGGCTCAT AAAGCTTCTG AGACAGGGCT ACACCATCCG CATCTTACTG 
TGGACCTTCG TGCAGTCCTT TAAGGCTCTT CCCTACGTCT GCCTTCTCAT TGCCATGCTT 
TTCTTCATCT ACGCCATCAT TGGGATGCAG GTGTTTGGAA ACATAAAGTT AGATGAGGAG 
AGTCACATCA ACCGGCACAA CAACTTCCGG AGTTTCTTTG GATCCCTCAT GCTGCTTTTC 
AGGAGTGCCA CGGGGGAGGC TTGGCAGGAG ATTATGCTGT CCTGTCTCGG TGAGAAGGGT 
TGTGAGCCTG ACACCACTGC ACCCTCAGGG CAGAATGAGA GTGAGCGCTG TGGCACTGAC 
CTGGCCTACG TTTATTTTGT CTCCTTCATC TTCTTCTGCT CCTTCCTGAT GCTCAACCTG 
TTCGTGGCTG TCATCATGGA CAACTTTGAG TACCTGACTC GAGATTCCTC CATCCTGGGG 
CCTCACCACT TAGATGAGTT TGTCCGTGTC TGGGCAGAAT ATGACAGAGC AGCATGTGGC 
CGCATCCATT ACACTGAGAT GTATGAAATG CTGACTCTCA TGTCACCACC GCTAGGCCTC 
GGCAAGAGAT GTCCCTCCAA AGTGGCCTAT AAGAGGCTGG TCCTGATGAA CATGCCAGTG 
GCTGAGGACA TGACAGTCCA CTTCACCTCC ACACTTATGG CTCTGATCCG GACAGCTCTG 
GACATTAAAA TCGCCAAAGG TGGTGCAGAC AGACAGCAGC TAGACTCGGA GCTGCAAAAA 
GAGACCCTGG CCATTTGGCC TCACCTGTCC CAAAAGATGT TGGATCTGCT TGTGCCCATG 
CCCAAAGCCT CTGACCTGAC TGTGGGCAAG ATCTATGCAG CAATGATGAT CATGGACTAC 
TATAAACAGA GTAAGGTAAA GAAACAGAGA CAGCAGCTGG AAGAACAGAA AAATGCACCC 
ATGTTCCAGC GCATGGAGCC CTCATCACTG CCTCAGGAGA TCATTGCTAA TGCCAAAGCC 
CTGCCTTATC TCCAGCAGGA CCCTGTTTCT GGCCTGAGTG GTCGGAGTGG ATACCCTTCA 
ATGAGTCCAC TCTCCCCTCA AGAAATATTC CAGTTGGCTT GTATGGACCC AGCGGATGAT 
GGACAATTCC AGGAACAACA ATCTCTGGTG GTAACAGACC CTAGCTCTAT GCGACGTTCA 
TTTTCTACAA TTCGGGATAA GCGTTCAAAT TCCTCCTGGT TGGAGGAGTT CTCCATGGAA 
CGCAGCAGTG AAAACACTTA CAAGTCGCGG CGCAGAAGTT ACCATTCCTC CCTGAGGCTG 
TCAGCACACC GCCTGAATTC TGACTCTGGT CACAAGTCTG ACACTCACCG ATCAGGAGGC 
AGGGAGAGAG GCCGATCCAA AGAACGAAAG CACCTCCTCT CTCCAGATGT CTCCCGCTGC 
AATTCTGAAG AGCGAGGGAC CCAGGCCGAC TGGGAGTCCC CAGAACGTCG CCAGTCCAGG 
TCACCCAGTG AAGGAAGGTC ACAGACCCCC AATAGACAGG GTACCGGTTC CCTAAGTGAG 
AGCTCCATCC CCTCTATTTC CGACACCAGC ACCCCAAGAC GAAGCCGTCG CCAGCTCCCA 
CCTGTGCCAC CAAAGCCTCG TCCCCTCCTC TCCTACAGCT CCCTGATGAG ACACACTGGC 
GGCATCTCTC CACCTCCTGA TGGAAGTGAG GGTGGATCCC CACTGGCCTC TCAAGCTCTG 
GAGAGCAACA GCGCTTGCCT GACCGAGTCT TCCAACTCCT TGCACCCCCA GCAGGGCCAG 
CATCCTTCCC CACAGCACTA CATCTCCGAG CCCTATCTGG CCCTCCATGA AGACTCCCAC 
GCCTCAGACT GTGGCGAGGA GGAGACACTC ACCTTTGAGG CAGCCGTAGC CACTAGCTTG 
GGCCGGTCCA ATACCATTGG CTCTGCGCCA CCCCTACGGC ACAGCTGGCA GATGCCTAAT 
GGGCACTATC GGCGGCGGAG GCGTGGGGGG CCTGGGCCTG GCATGATGTG TGGAGCTGTC 
AGTGACCTCC TGAGTGACAC GGAAGAAGAC GATAAGTGCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_033912.2
CDS	2..6823
Translation	MARFGEAVVVGRPGSGDGDSDQSRNRQGTPVPASGPAAAYKQSKAQRARTMALYNPIPVRQNCFTVNRSLFIFGEDNIVRKYAKKLIDWPPFEYMILATIIANCIVLALEQHLPEDDKTPMSRRLEKTEPYFIGIFCFEAGIKIVALGFIFHKGSYLRNGWNVMDFIVVLSGILATAGTHFNTHVDLRTLRAVRVLRPLKLVSGIPSLQIVLKSIMKAMVPLLQIGLLLFFAILMFAIIGLEFYSGKLHRACFMNNSGILEGFDPPHPCGVQGCPAGYECKDWIGPNDGITQFDNILFAVLTVFQCITMEGWTTVLYNTNDALGATWNWLYFIPLIIIGSFFVLNLVLGVLSGEFAKERERVENRRAFMKLRRQQQIERELNGYRAWIDKAEEVMLAEENKNSGTSALEVLRRATIKRSRTEAMTRDSSDEHCVDISSVGTPLARASIKSTKVDGASYFRHKERLLRISIRHMVKSQVFYWIVLSVVALNTACVAIVHHNQPQWLTHLLYYAEFLFLGLFLLEMSLKMYGMGPRLYFHSSFNCFDFGVTVGSIFEVVWAIFRPGTSFGISVLRALRLLRIFKITKYWASLRNLVVSLMSSMKSIISLLFLLFLFIVVFALLGMQLFGGRFNFNDGTPSANFDTFPAAIMTVFQILTGEDWNEVMYNGIRSQGGVSSGMWSAIYFIVLTLFGNYTLLNVFLAIAVDNLANAQELTKDEQEEEEAFNQKHALQKAKEVSPMSAPNMPSIERDRRRRHHMSMWEPRSSHLRERRRRHHMSVWEQRTSQLRRHMQMSSQEALNKEEAPPMNPLNPLNPLSPLNPLNAHPSLYRRPRPIEGLALGLGLEKCEEERISRGGSLKGDIGGLTSALDNQRSPLSLGKREPPWLPRSCHGNCDPIQQEAGGGETVVTFEDRARHRQSQRRSRHRRVRTEGKDSASASRSRSASQERSLDEGVSVEGEKEHEPHSSHRSKEPTIHEEERTQDLRRTNSLMVPRGSGLVGALDEAETPLVQPQPELEVGKDAALTEQEAEGSSEQALLGDVQLDVGRGISQSEPDLSCMTANMDKATTESTSVTVAIPDVDPLVDSTVVNISNKTDGEASPLKEAETKEEEEEVEKKKKQKKEKRETGKAMVPHSSMFIFSTTNPIRRACHYIVNLRYFEMCILLVIAASSIALAAEDPVLTNSERNKVLRYFDYVFTGVFTFEMVIKMIDQGLILQDGSYFRDLWNILDFVVVVGALVAFALANALGTNKGRDIKTIKSLRVLRVLRPLKTIKRLPKLKAVFDCVVTSLKNVFNILIVYKLFMFIFAVIAVQLFKGKFFYCTDSSKDTEKECIGNYVDHEKNKMEVKGREWKRHEFHYDNIIWALLTLFTVSTGEGWPQVLQHSVDVTEEDRGPSRSNRMEMSIFYVVYFVVFPFFFVNIFVALIIITFQEQGDKMMEECSLEKNERACIDFAISAKPLTRYMPQNRHTFQYRVWHFVVSPSFEYTIMAMIALNTVVLMMKYYSAPCTYELALKYLNIAFTMVFSLECVLKVIAFGFLNYFRDTWNIFDFITVIGSITEIILTDSKLVNTSGFNMSFLKLFRAARLIKLLRQGYTIRILLWTFVQSFKALPYVCLLIAMLFFIYAIIGMQVFGNIKLDEESHINRHNNFRSFFGSLMLLFRSATGEAWQEIMLSCLGEKGCEPDTTAPSGQNESERCGTDLAYVYFVSFIFFCSFLMLNLFVAVIMDNFEYLTRDSSILGPHHLDEFVRVWAEYDRAACGRIHYTEMYEMLTLMSPPLGLGKRCPSKVAYKRLVLMNMPVAEDMTVHFTSTLMALIRTALDIKIAKGGADRQQLDSELQKETLAIWPHLSQKMLDLLVPMPKASDLTVGKIYAAMMIMDYYKQSKVKKQRQQLEEQKNAPMFQRMEPSSLPQEIIANAKALPYLQQDPVSGLSGRSGYPSMSPLSPQEIFQLACMDPADDGQFQEQQSLVVTDPSSMRRSFSTIRDKRSNSSWLEEFSMERSSENTYKSRRRSYHSSLRLSAHRLNSDSGHKSDTHRSGGRERGRSKERKHLLSPDVSRCNSEERGTQADWESPERRQSRSPSEGRSQTPNRQGTGSLSESSIPSISDTSTPRRSRRQLPPVPPKPRPLLSYSSLMRHTGGISPPPDGSEGGSPLASQALESNSACLTESSNSLHPQQGQHPSPQHYISEPYLALHEDSHASDCGEEETLTFEAAVATSLGRSNTIGSAPPLRHSWQMPNGHYRRRRRGGPGPGMMCGAVSDLLSDTEEDDKC

Target ORF information:

RefSeq Version	NM_009782.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus calcium channel, voltage-dependent, R type, alpha 1E subunit (Cacna1e), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_009782.3

ORF Insert Sequence:

ATGGCTCGCT TCGGGGAGGC GGTGGTCGTT GGCAGGCCAG GCTCAGGCGA TGGAGACTCG 
GACCAGAGCA GGAACCGACA AGGAACCCCC GTCCCGGCCT CGGGGCCGGC GGCCGCCTAC 
AAGCAGTCAA AAGCGCAGAG GGCGCGGACT ATGGCTTTGT ACAACCCCAT ACCAGTCCGG 
CAGAACTGTT TCACGGTCAA CAGATCCCTG TTCATCTTCG GAGAAGATAA CATTGTCAGG 
AAATACGCCA AGAAGCTCAT CGATTGGCCG CCATTTGAGT ACATGATCCT GGCCACCATC 
ATTGCCAACT GCATCGTCTT GGCCCTGGAG CAGCATCTTC CTGAGGATGA CAAGACCCCA 
ATGTCTCGAA GACTGGAGAA GACAGAACCA TATTTCATTG GGATCTTCTG CTTTGAAGCT 
GGGATCAAAA TTGTGGCTCT AGGGTTCATC TTCCATAAGG GTTCATACCT CCGTAATGGC 
TGGAATGTCA TGGACTTCAT CGTGGTCCTT AGTGGCATCC TGGCCACTGC GGGAACCCAC 
TTCAACACCC ACGTGGACCT GCGGACCCTC CGGGCTGTGC GTGTCCTGAG GCCTTTAAAG 
CTGGTATCAG GAATACCTAG CCTGCAGATC GTGCTGAAGT CTATCATGAA GGCCATGGTG 
CCTCTTCTGC AGATTGGCCT TCTGCTCTTC TTTGCCATCC TCATGTTTGC TATCATTGGC 
TTGGAGTTCT ACAGTGGCAA GTTGCATCGA GCATGCTTCA TGAACAATTC AGGTATTCTA 
GAGGGGTTTG ATCCTCCTCA CCCGTGTGGT GTGCAAGGCT GCCCGGCTGG TTATGAATGT 
AAGGACTGGA TCGGCCCCAA TGACGGGATC ACCCAGTTTG ACAACATCCT TTTTGCCGTG 
CTGACTGTCT TCCAATGCAT CACTATGGAA GGGTGGACCA CTGTGCTGTA CAATACCAAT 
GATGCCTTAG GAGCCACCTG GAACTGGCTG TACTTCATCC CTCTCATCAT CATCGGATCC 
TTCTTTGTTC TCAACCTCGT CCTGGGAGTG CTTTCCGGGG AATTTGCCAA AGAAAGAGAG 
AGAGTGGAGA ACCGAAGAGC GTTCATGAAG CTCCGGCGCC AGCAACAGAT TGAGCGAGAA 
TTGAACGGCT ACCGTGCCTG GATAGACAAA GCAGAGGAAG TCATGCTTGC TGAAGAAAAT 
AAAAACTCGG GAACATCAGC CTTGGAAGTG CTTCGAAGGG CAACCATCAA AAGGAGCCGG 
ACGGAGGCCA TGACCCGAGA CTCCAGCGAT GAGCACTGCG TTGATATCTC TTCAGTGGGC 
ACACCTCTTG CCAGAGCCAG TATCAAGAGC ACAAAAGTAG ATGGGGCCTC CTATTTCCGG 
CACAAGGAAC GGCTTCTGCG CATCTCTATC CGCCACATGG TCAAGTCCCA AGTCTTTTAC 
TGGATCGTCC TGAGTGTTGT GGCTCTTAAT ACTGCCTGTG TGGCCATTGT TCATCACAAC 
CAGCCACAGT GGCTCACTCA CCTCCTCTAC TATGCAGAGT TTTTGTTTCT GGGACTCTTC 
CTGTTGGAGA TGTCCCTGAA GATGTATGGC ATGGGGCCAC GCCTCTATTT TCACTCATCC 
TTCAACTGCT TTGATTTTGG GGTCACAGTG GGCAGCATCT TTGAGGTTGT CTGGGCAATC 
TTCAGACCTG GCACATCTTT TGGAATCAGT GTCTTACGAG CTCTCCGGCT CCTAAGGATA 
TTTAAAATAA CCAAGTATTG GGCATCCCTC CGGAATCTGG TTGTCTCCTT GATGAGCTCC 
ATGAAGTCTA TCATCAGCTT ACTCTTCCTT CTCTTCCTCT TCATCGTTGT CTTTGCTCTC 
CTGGGAATGC AGCTCTTTGG AGGCAGGTTT AACTTTAATG ATGGGACTCC TTCAGCTAAT 
TTTGACACCT TCCCTGCAGC TATCATGACA GTGTTCCAGA TCCTGACAGG GGAAGACTGG 
AATGAGGTGA TGTACAATGG CATTCGCTCC CAGGGTGGGG TCAGCTCAGG CATGTGGTCT 
GCCATCTACT TCATTGTGCT CACCTTGTTT GGAAACTACA CGCTGCTAAA TGTATTCTTG 
GCCATCGCTG TGGACAATCT GGCCAATGCC CAGGAACTGA CCAAGGATGA GCAGGAGGAA 
GAAGAAGCCT TCAACCAGAA ACATGCACTG CAGAAGGCCA AGGAGGTCAG CCCGATGTCT 
GCTCCCAACA TGCCTTCCAT TGAAAGAGAC AGAAGGAGAA GACACCACAT GTCGATGTGG 
GAGCCACGCA GCAGCCACCT GAGGGAGCGG AGGCGCCGGC ACCACATGTC TGTGTGGGAA 
CAACGCACCA GCCAGCTGAG GAGGCACATG CAAATGTCTA GCCAAGAAGC CCTCAACAAA 
GAGGAGGCCC CGCCAATGAA CCCCCTCAAT CCACTCAACC CACTGAGTCC TCTCAACCCC 
CTCAATGCTC ACCCCAGCCT TTATCGGAGG CCCAGGCCCA TTGAGGGCCT AGCCCTAGGT 
CTGGGTCTTG AAAAGTGTGA AGAAGAACGC ATCAGTCGTG GGGGCTCCCT CAAGGGGGAC 
ATAGGGGGCC TGACTAGCGC CCTGGACAAC CAGAGGAGCC CCTTGTCCTT GGGCAAACGG 
GAGCCACCGT GGCTGCCTAG ATCCTGTCAT GGAAACTGTG ACCCAATCCA GCAGGAGGCT 
GGGGGAGGAG AGACTGTGGT GACTTTTGAG GACCGGGCCA GGCACAGGCA GAGTCAGAGG 
CGAAGCCGGC ATCGCCGAGT CAGGACTGAG GGCAAGGACT CTGCCTCTGC ATCCCGAAGC 
AGGTCTGCCA GCCAGGAGCG GAGTCTGGAT GAAGGGGTGT CCGTTGAGGG GGAGAAGGAA 
CATGAGCCTC ACAGCAGCCA CAGGAGCAAA GAGCCGACCA TCCACGAAGA GGAGAGAACC 
CAGGACTTAA GGAGGACCAA CAGTCTGATG GTCCCCAGGG GCTCTGGACT GGTAGGGGCT 
CTTGATGAAG CAGAAACACC TCTAGTCCAG CCCCAACCTG AGCTGGAAGT GGGGAAGGAT 
GCAGCTCTGA CAGAGCAGGA GGCTGAAGGC AGCAGTGAAC AAGCCCTTCT TGGGGACGTA 
CAGCTGGATG TGGGCCGGGG CATTAGCCAG AGTGAACCGG ATCTCTCCTG CATGACAGCC 
AACATGGACA AGGCTACCAC GGAGAGCACC AGTGTCACGG TCGCCATCCC TGATGTGGAC 
CCCTTGGTGG ACTCGACAGT GGTGAACATT AGCAACAAGA CGGATGGAGA AGCCAGTCCC 
TTGAAGGAGG CAGAGACTAA AGAGGAAGAA GAAGAGGTAG AGAAGAAGAA GAAGCAGAAG 
AAGGAGAAGC GAGAGACTGG AAAAGCCATG GTGCCCCATA GCTCTATGTT CATCTTCAGC 
ACCACTAACC CGATCCGCAG GGCCTGCCAC TACATTGTGA ACCTACGCTA CTTTGAAATG 
TGCATTCTCT TGGTGATTGC GGCCAGCAGC ATTGCCCTGG CAGCCGAGGA CCCTGTACTA 
ACTAACTCGG AGCGTAACAA AGTCCTGAGA TATTTCGACT ATGTTTTCAC AGGAGTGTTT 
ACCTTTGAGA TGGTTATAAA GATGATAGAC CAAGGCTTGA TCCTGCAGGA TGGATCATAC 
TTCAGAGACC TCTGGAACAT CCTGGACTTT GTAGTAGTGG TTGGTGCATT GGTAGCATTT 
GCTCTGGCGA ACGCTTTGGG AACCAACAAG GGGAGGGATA TCAAGACTAT CAAGTCTCTG 
AGGGTACTTC GAGTTCTGCG GCCACTGAAA ACCATCAAGC GCTTGCCCAA GCTGAAGGCT 
GTGTTTGACT GCGTGGTGAC CTCCTTGAAG AATGTCTTCA ACATACTCAT TGTCTACAAG 
CTCTTCATGT TCATCTTTGC CGTCATCGCT GTTCAGCTAT TCAAAGGAAA GTTCTTTTAT 
TGCACTGACA GTTCCAAGGA CACAGAGAAG GAGTGCATAG GCAACTATGT AGACCACGAG 
AAAAACAAGA TGGAGGTGAA GGGCCGGGAA TGGAAGCGTC ATGAATTCCA CTATGACAAC 
ATCATCTGGG CCCTGCTTAC CCTCTTCACA GTCTCTACAG GGGAAGGATG GCCTCAGGTT 
CTGCAGCACT CTGTAGATGT GACAGAGGAG GACAGAGGCC CAAGTCGCAG CAACCGCATG 
GAGATGTCCA TCTTTTATGT GGTCTACTTT GTGGTCTTCC CTTTCTTCTT TGTCAATATC 
TTCGTGGCTC TCATTATCAT CACCTTCCAG GAGCAAGGGG ACAAGATGAT GGAGGAGTGT 
AGCCTGGAGA AGAATGAGCG GGCATGCATC GACTTCGCCA TTAGTGCCAA ACCTCTCACC 
CGCTACATGC CACAGAACAG GCACACCTTC CAGTACCGTG TGTGGCACTT TGTAGTCTCC 
CCATCCTTTG AGTATACCAT CATGGCCATG ATTGCCTTGA ACACTGTCGT GCTGATGATG 
AAGTACTACT CTGCTCCTTG CACCTATGAG CTCGCCTTAA AGTACCTGAA CATCGCCTTC 
ACTATGGTGT TTTCCCTGGA GTGTGTCCTA AAGGTCATTG CTTTCGGCTT CCTGAACTAT 
TTCCGAGATA CCTGGAACAT CTTTGACTTC ATCACGGTGA TCGGCAGTAT CACAGAAATT 
ATCCTGACAG ATAGCAAGCT GGTGAACACC AGTGGCTTCA ATATGAGCTT TCTGAAGCTC 
TTCCGAGCTG CACGGCTCAT AAAGCTTCTG AGACAGGGCT ACACCATCCG CATCTTACTG 
TGGACCTTCG TGCAGTCCTT TAAGGCTCTT CCCTACGTCT GCCTTCTCAT TGCCATGCTT 
TTCTTCATCT ACGCCATCAT TGGGATGCAG GTGTTTGGAA ACATAAAGTT AGATGAGGAG 
AGTCACATCA ACCGGCACAA CAACTTCCGG AGTTTCTTTG GATCCCTCAT GCTGCTTTTC 
AGGAGTGCCA CGGGGGAGGC TTGGCAGGAG ATTATGCTGT CCTGTCTCGG TGAGAAGGGT 
TGTGAGCCTG ACACCACTGC ACCCTCAGGG CAGAATGAGA GTGAGCGCTG TGGCACTGAC 
CTGGCCTACG TTTATTTTGT CTCCTTCATC TTCTTCTGCT CCTTCCTGAT GCTCAACCTG 
TTCGTGGCTG TCATCATGGA CAACTTTGAG TACCTGACTC GAGATTCCTC CATCCTGGGG 
CCTCACCACT TAGATGAGTT TGTCCGTGTC TGGGCAGAAT ATGACAGAGC AGCATGTGGC 
CGCATCCATT ACACTGAGAT GTATGAAATG CTGACTCTCA TGTCACCACC GCTAGGCCTC 
GGCAAGAGAT GTCCCTCCAA AGTGGCCTAT AAGAGGCTGG TCCTGATGAA CATGCCAGTG 
GCTGAGGACA TGACAGTCCA CTTCACCTCC ACACTTATGG CTCTGATCCG GACAGCTCTG 
GACATTAAAA TCGCCAAAGG TGGTGCAGAC AGACAGCAGC TAGACTCGGA GCTGCAAAAA 
GAGACCCTGG CCATTTGGCC TCACCTGTCC CAAAAGATGT TGGATCTGCT TGTGCCCATG 
CCCAAAGCCT CTGACCTGAC TGTGGGCAAG ATCTATGCAG CAATGATGAT CATGGACTAC 
TATAAACAGA GTAAGGTAAA GAAACAGAGA CAGCAGCTGG AAGAACAGAA AAATGCACCC 
ATGTTCCAGC GCATGGAGCC CTCATCACTG CCTCAGGAGA TCATTGCTAA TGCCAAAGCC 
CTGCCTTATC TCCAGCAGGA CCCTGTTTCT GGCCTGAGTG GTCGGAGTGG ATACCCTTCA 
ATGAGTCCAC TCTCCCCTCA AGAAATATTC CAGTTGGCTT GTATGGACCC AGCGGATGAT 
GGACAATTCC AGGAACAACA ATCTCTGGTG GTAACAGACC CTAGCTCTAT GCGACGTTCA 
TTTTCTACAA TTCGGGATAA GCGTTCAAAT TCCTCCTGGT TGGAGGAGTT CTCCATGGAA 
CGCAGCAGTG AAAACACTTA CAAGTCGCGG CGCAGAAGTT ACCATTCCTC CCTGAGGCTG 
TCAGCACACC GCCTGAATTC TGACTCTGGT CACAAGTCTG ACACTCACCG ATCAGGAGGC 
AGGGAGAGAG GCCGATCCAA AGAACGAAAG CACCTCCTCT CTCCAGATGT CTCCCGCTGC 
AATTCTGAAG AGCGAGGGAC CCAGGCCGAC TGGGAGTCCC CAGAACGTCG CCAGTCCAGG 
TCACCCAGTG AAGGAAGGTC ACAGACCCCC AATAGACAGG GTACCGGTTC CCTAAGTGAG 
AGCTCCATCC CCTCTATTTC CGACACCAGC ACCCCAAGAC GAAGCCGTCG CCAGCTCCCA 
CCTGTGCCAC CAAAGCCTCG TCCCCTCCTC TCCTACAGCT CCCTGATGAG ACACACTGGC 
GGCATCTCTC CACCTCCTGA TGGAAGTGAG GGTGGATCCC CACTGGCCTC TCAAGCTCTG 
GAGAGCAACA GCGCTTGCCT GACCGAGTCT TCCAACTCCT TGCACCCCCA GCAGGGCCAG 
CATCCTTCCC CACAGCACTA CATCTCCGAG CCCTATCTGG CCCTCCATGA AGACTCCCAC 
GCCTCAGACT GTGGCGAGGA GGAGACACTC ACCTTTGAGG CAGCCGTAGC CACTAGCTTG 
GGCCGGTCCA ATACCATTGG CTCTGCGCCA CCCCTACGGC ACAGCTGGCA GATGCCTAAT 
GGGCACTATC GGCGGCGGAG GCGTGGGGGG CCTGGGCCTG GCATGATGTG TGGAGCTGTC 
AGTGACCTCC TGAGTGACAC GGAAGAAGAC GATAAGTGCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cacna1e ( XM_017312619.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017168108.1 Mus musculus calcium channel, voltage-dependent, R type, alpha 1E subunit (Cacna1e), transcript variant X6, mRNA.

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CloneID	OMu27870
Clone ID Related Accession (Same CDS sequence)	XM_017312619.1
Accession Version	XM_017312619.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7389bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	voltage-dependent R-type calcium channel subunit alpha-1E isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGCCCG AGATGCGTGG ACTGAGCCCA GGCCGAAGGC TGCCCGAGCC CACCCTGTGC 
CCGGCTACAG GAGGGAGCCC TGAGCCCGGG GCTGCTGGCA GGCGCCGCCA CGTCCTGGCT 
CTGCCTGATG AGGAGGCATG GCGGCCGCAG CAGTGCGCCC TGCTCGAGGC CAATGCCTCC 
GACGAGGTGG GCATGTTGCC TGCTTCCCCG CGCTCCGCGG TGGCCGGCTT CGACAACTTC 
TTCCCGGTGC AGGAAGGCGA GGGCCCGGGC TGGGAGGGCA GCACCACGCT GGATGCAGGC 
TCCAGCCCCT TCCTTCCTGT GAGCCCAGAG GTCATGAAGC GGAGGCGAGG GGGGCTCATC 
GAGCAGCGGG ACATCATCAA GGCCCACGAG GCACACAAGA TGCAGAGCAC CCCGCAGGCC 
CGGCGCAAGG AGTGGGAGAT GGCTCGCTTC GGGGAGGCGG TGGTCGTTGG CAGGCCAGGC 
TCAGGCGATG GAGACTCGGA CCAGAGCAGG AACCGACAAG GAACCCCCGT CCCGGCCTCG 
GGGCCGGCGG CCGCCTACAA GCAGTCAAAA GCGCAGAGGG CGCGGACTAT GGCTTTGTAC 
AACCCCATAC CAGTCCGGCA GAACTGTTTC ACGGTCAACA GATCCCTGTT CATCTTCGGA 
GAAGATAACA TTGTCAGGAA ATACGCCAAG AAGCTCATCG ATTGGCCGCC ATTTGAGTAC 
ATGATCCTGG CCACCATCAT TGCCAACTGC ATCGTCTTGG CCCTGGAGCA GCATCTTCCT 
GAGGATGACA AGACCCCAAT GTCTCGAAGA CTGGAGAAGA CAGAACCATA TTTCATTGGG 
ATCTTCTGCT TTGAAGCTGG GATCAAAATT GTGGCTCTAG GGTTCATCTT CCATAAGGGT 
TCATACCTCC GTAATGGCTG GAATGTCATG GACTTCATCG TGGTCCTTAG TGGCATCCTG 
GCCACTGCGG GAACCCACTT CAACACCCAC GTGGACCTGC GGACCCTCCG GGCTGTGCGT 
GTCCTGAGGC CTTTAAAGCT GGTATCAGGA ATACCTAGCC TGCAGATCGT GCTGAAGTCT 
ATCATGAAGG CCATGGTGCC TCTTCTGCAG ATTGGCCTTC TGCTCTTCTT TGCCATCCTC 
ATGTTTGCTA TCATTGGCTT GGAGTTCTAC AGTGGCAAGT TGCATCGAGC ATGCTTCATG 
AACAATTCAG GTATTCTAGA GGGGTTTGAT CCTCCTCACC CGTGTGGTGT GCAAGGCTGC 
CCGGCTGGTT ATGAATGTAA GGACTGGATC GGCCCCAATG ACGGGATCAC CCAGTTTGAC 
AACATCCTTT TTGCCGTGCT GACTGTCTTC CAATGCATCA CTATGGAAGG GTGGACCACT 
GTGCTGTACA ATACCAATGA TGCCTTAGGA GCCACCTGGA ACTGGCTGTA CTTCATCCCT 
CTCATCATCA TCGGATCCTT CTTTGTTCTC AACCTCGTCC TGGGAGTGCT TTCCGGGGAA 
TTTGCCAAAG AAAGAGAGAG AGTGGAGAAC CGAAGAGCGT TCATGAAGCT CCGGCGCCAG 
CAACAGATTG AGCGAGAATT GAACGGCTAC CGTGCCTGGA TAGACAAAGC AGAGGAAGTC 
ATGCTTGCTG AAGAAAATAA AAACTCGGGA ACATCAGCCT TGGAAGTGCT TCGAAGGGCA 
ACCATCAAAA GGAGCCGGAC GGAGGCCATG ACCCGAGACT CCAGCGATGA GCACTGCGTT 
GATATCTCTT CAGTGGGCAC ACCTCTTGCC AGAGCCAGTA TCAAGAGCAC AAAAGTAGAT 
GGGGCCTCCT ATTTCCGGCA CAAGGAACGG CTTCTGCGCA TCTCTATCCG CCACATGGTC 
AAGTCCCAAG TCTTTTACTG GATCGTCCTG AGTGTTGTGG CTCTTAATAC TGCCTGTGTG 
GCCATTGTTC ATCACAACCA GCCACAGTGG CTCACTCACC TCCTCTACTA TGCAGAGTTT 
TTGTTTCTGG GACTCTTCCT GTTGGAGATG TCCCTGAAGA TGTATGGCAT GGGGCCACGC 
CTCTATTTTC ACTCATCCTT CAACTGCTTT GATTTTGGGG TCACAGTGGG CAGCATCTTT 
GAGGTTGTCT GGGCAATCTT CAGACCTGGC ACATCTTTTG GAATCAGTGT CTTACGAGCT 
CTCCGGCTCC TAAGGATATT TAAAATAACC AAGTATTGGG CATCCCTCCG GAATCTGGTT 
GTCTCCTTGA TGAGCTCCAT GAAGTCTATC ATCAGCTTAC TCTTCCTTCT CTTCCTCTTC 
ATCGTTGTCT TTGCTCTCCT GGGAATGCAG CTCTTTGGAG GCAGGTTTAA CTTTAATGAT 
GGGACTCCTT CAGCTAATTT TGACACCTTC CCTGCAGCTA TCATGACAGT GTTCCAGATC 
CTGACAGGGG AAGACTGGAA TGAGGTGATG TACAATGGCA TTCGCTCCCA GGGTGGGGTC 
AGCTCAGGCA TGTGGTCTGC CATCTACTTC ATTGTGCTCA CCTTGTTTGG AAACTACACG 
CTGCTAAATG TATTCTTGGC CATCGCTGTG GACAATCTGG CCAATGCCCA GGAACTGACC 
AAGGATGAGC AGGAGGAAGA AGAAGCCTTC AACCAGAAAC ATGCACTGCA GAAGGCCAAG 
GAGGTCAGCC CGATGTCTGC TCCCAACATG CCTTCCATTG AAAGAGACAG AAGGAGAAGA 
CACCACATGT CGATGTGGGA GCCACGCAGC AGCCACCTGA GGGAGCGGAG GCGCCGGCAC 
CACATGTCTG TGTGGGAACA ACGCACCAGC CAGCTGAGGA GGCACATGCA AATGTCTAGC 
CAAGAAGCCC TCAACAAAGA GGAGGCCCCG CCAATGAACC CCCTCAATCC ACTCAACCCA 
CTGAGTCCTC TCAACCCCCT CAATGCTCAC CCCAGCCTTT ATCGGAGGCC CAGGCCCATT 
GAGGGCCTAG CCCTAGGTCT GGGTCTTGAA AAGTGTGAAG AAGAACGCAT CAGTCGTGGG 
GGCTCCCTCA AGGGGGACAT AGGGGGCCTG ACTAGCGCCC TGGACAACCA GAGGAGCCCC 
TTGTCCTTGG GCAAACGGGA GCCACCGTGG CTGCCTAGAT CCTGTCATGG AAACTGTGAC 
CCAATCCAGC AGGAGGCTGG GGGAGGAGAG ACTGTGGTGA CTTTTGAGGA CCGGGCCAGG 
CACAGGCAGA GTCAGAGGCG AAGCCGGCAT CGCCGAGTCA GGACTGAGGG CAAGGACTCT 
GCCTCTGCAT CCCGAAGCAG GTCTGCCAGC CAGGAGCGGA GTCTGGATGA AGGGGTGTCC 
GTTGAGGGGG AGAAGGAACA TGAGCCTCAC AGCAGCCACA GGAGCAAAGA GCCGACCATC 
CACGAAGAGG AGAGAACCCA GGACTTAAGG AGGACCAACA GTCTGATGGT CCCCAGGGGC 
TCTGGACTGG TAGGGGCTCT TGATGAAGCA GAAACACCTC TAGTCCAGCC CCAACCTGAG 
CTGGAAGTGG GGAAGGATGC AGCTCTGACA GAGCAGGAGG CTGAAGGCAG CAGTGAACAA 
GCCCTTCTTG GGGACGTACA GCTGGATGTG GGCCGGGGCA TTAGCCAGAG TGAACCGGAT 
CTCTCCTGCA TGACAGCCAA CATGGACAAG GCTACCACGG AGAGCACCAG TGTCACGGTC 
GCCATCCCTG ATGTGGACCC CTTGGTGGAC TCGACAGTGG TGAACATTAG CAACAAGACG 
GATGGAGAAG CCAGTCCCTT GAAGGAGGCA GAGACTAAAG AGGAAGAAGA AGAGGTAGAG 
AAGAAGAAGA AGCAGAAGAA GGAGAAGCGA GAGACTGGAA AAGCCATGGT GCCCCATAGC 
TCTATGTTCA TCTTCAGCAC CACTAACCCG ATCCGCAGGG CCTGCCACTA CATTGTGAAC 
CTACGCTACT TTGAAATGTG CATTCTCTTG GTGATTGCGG CCAGCAGCAT TGCCCTGGCA 
GCCGAGGACC CTGTACTAAC TAACTCGGAG CGTAACAAAG TCCTGAGATA TTTCGACTAT 
GTTTTCACAG GAGTGTTTAC CTTTGAGATG GTTATAAAGA TGATAGACCA AGGCTTGATC 
CTGCAGGATG GATCATACTT CAGAGACCTC TGGAACATCC TGGACTTTGT AGTAGTGGTT 
GGTGCATTGG TAGCATTTGC TCTGGCGAAC GCTTTGGGAA CCAACAAGGG GAGGGATATC 
AAGACTATCA AGTCTCTGAG GGTACTTCGA GTTCTGCGGC CACTGAAAAC CATCAAGCGC 
TTGCCCAAGC TGAAGGCTGT GTTTGACTGC GTGGTGACCT CCTTGAAGAA TGTCTTCAAC 
ATACTCATTG TCTACAAGCT CTTCATGTTC ATCTTTGCCG TCATCGCTGT TCAGCTATTC 
AAAGGAAAGT TCTTTTATTG CACTGACAGT TCCAAGGACA CAGAGAAGGA GTGCATAGGC 
AACTATGTAG ACCACGAGAA AAACAAGATG GAGGTGAAGG GCCGGGAATG GAAGCGTCAT 
GAATTCCACT ATGACAACAT CATCTGGGCC CTGCTTACCC TCTTCACAGT CTCTACAGGG 
GAAGGATGGC CTCAGGTTCT GCAGCACTCT GTAGATGTGA CAGAGGAGGA CAGAGGCCCA 
AGTCGCAGCA ACCGCATGGA GATGTCCATC TTTTATGTGG TCTACTTTGT GGTCTTCCCT 
TTCTTCTTTG TCAATATCTT CGTGGCTCTC ATTATCATCA CCTTCCAGGA GCAAGGGGAC 
AAGATGATGG AGGAGTGTAG CCTGGAGAAG AATGAGCGGG CATGCATCGA CTTCGCCATT 
AGTGCCAAAC CTCTCACCCG CTACATGCCA CAGAACAGGC ACACCTTCCA GTACCGTGTG 
TGGCACTTTG TAGTCTCCCC ATCCTTTGAG TATACCATCA TGGCCATGAT TGCCTTGAAC 
ACTGTCGTGC TGATGATGAA GTACTACTCT GCTCCTTGCA CCTATGAGCT CGCCTTAAAG 
TACCTGAACA TCGCCTTCAC TATGGTGTTT TCCCTGGAGT GTGTCCTAAA GGTCATTGCT 
TTCGGCTTCC TGAACTATTT CCGAGATACC TGGAACATCT TTGACTTCAT CACGGTGATC 
GGCAGTATCA CAGAAATTAT CCTGACAGAT AGCAAGCTGG TGAACACCAG TGGCTTCAAT 
ATGAGCTTTC TGAAGCTCTT CCGAGCTGCA CGGCTCATAA AGCTTCTGAG ACAGGGCTAC 
ACCATCCGCA TCTTACTGTG GACCTTCGTG CAGTCCTTTA AGGCTCTTCC CTACGTCTGC 
CTTCTCATTG CCATGCTTTT CTTCATCTAC GCCATCATTG GGATGCAGGT GTTTGGAAAC 
ATAAAGTTAG ATGAGGAGAG TCACATCAAC CGGCACAACA ACTTCCGGAG TTTCTTTGGA 
TCCCTCATGC TGCTTTTCAG GAGTGCCACG GGGGAGGCTT GGCAGGAGAT TATGCTGTCC 
TGTCTCGGTG AGAAGGGTTG TGAGCCTGAC ACCACTGCAC CCTCAGGGCA GAATGAGAGT 
GAGCGCTGTG GCACTGACCT GGCCTACGTT TATTTTGTCT CCTTCATCTT CTTCTGCTCC 
TTCCTGATGC TCAACCTGTT CGTGGCTGTC ATCATGGACA ACTTTGAGTA CCTGACTCGA 
GATTCCTCCA TCCTGGGGCC TCACCACTTA GATGAGTTTG TCCGTGTCTG GGCAGAATAT 
GACAGAGCAG CATGTGGCCG CATCCATTAC ACTGAGATGT ATGAAATGCT GACTCTCATG 
TCACCACCGC TAGGCCTCGG CAAGAGATGT CCCTCCAAAG TGGCCTATAA GAGGCTGGTC 
CTGATGAACA TGCCAGTGGC TGAGGACATG ACAGTCCACT TCACCTCCAC ACTTATGGCT 
CTGATCCGGA CAGCTCTGGA CATTAAAATC GCCAAAGGTG GTGCAGACAG ACAGCAGCTA 
GACTCGGAGC TGCAAAAAGA GACCCTGGCC ATTTGGCCTC ACCTGTCCCA AAAGATGTTG 
GATCTGCTTG TGCCCATGCC CAAAGCCTCT GACCTGACTG TGGGCAAGAT CTATGCAGCA 
ATGATGATCA TGGACTACTA TAAACAGAGT AAGGTAAAGA AACAGAGACA GCAGCTGGAA 
GAACAGAAAA ATGCACCCAT GTTCCAGCGC ATGGAGCCCT CATCACTGCC TCAGGAGATC 
ATTGCTAATG CCAAAGCCCT GCCTTATCTC CAGCAGGACC CTGTTTCTGG CCTGAGTGGT 
CGGAGTGGAT ACCCTTCAAT GAGTCCACTC TCCCCTCAAG AAATATTCCA GTTGGCTTGT 
ATGGACCCAG CGGATGATGG ACAATTCCAG GAACAACAAT CTCTGGAGCC AGAGGTTAGT 
GAATTAAAAA GTGTGCAGTC CTCTAACCAT GGCATCTACC TTCCTCCGGA CACCCAGGAG 
CATGCGGGAT CTGGGAGGGC ATCCTCTATG CCACGTCTGA CTATGGATCC CCAGGTGGTA 
ACAGACCCTA GCTCTATGCG ACGTTCATTT TCTACAATTC GGGATAAGCG TTCAAATTCC 
TCCTGGTTGG AGGAGTTCTC CATGGAACGC AGCAGTGAAA ACACTTACAA GTCGCGGCGC 
AGAAGTTACC ATTCCTCCCT GAGGCTGTCA GCACACCGCC TGAATTCTGA CTCTGGTCAC 
AAGTCTGACA CTCACCGATC AGGAGGCAGG GAGAGAGGCC GATCCAAAGA ACGAAAGCAC 
CTCCTCTCTC CAGATGTCTC CCGCTGCAAT TCTGAAGAGC GAGGGACCCA GGCCGACTGG 
GAGTCCCCAG AACGTCGCCA GTCCAGGTCA CCCAGTGAAG GAAGGTCACA GACCCCCAAT 
AGACAGGGTA CCGGTTCCCT AAGTGAGAGC TCCATCCCCT CTATTTCCGA CACCAGCACC 
CCAAGACGAA GCCGTCGCCA GCTCCCACCT GTGCCACCAA AGCCTCGTCC CCTCCTCTCC 
TACAGCTCCC TGATGAGACA CACTGGCGGC ATCTCTCCAC CTCCTGATGG AAGTGAGGGT 
GGATCCCCAC TGGCCTCTCA AGCTCTGGAG AGCAACAGCG CTTGCCTGAC CGAGTCTTCC 
AACTCCTTGC ACCCCCAGCA GGGCCAGCAT CCTTCCCCAC AGCACTACAT CTCCGAGCCC 
TATCTGGCCC TCCATGAAGA CTCCCACGCC TCAGACTGTG GCGAGGAGGA GACACTCACC 
TTTGAGGCAG CCGTAGCCAC TAGCTTGGGC CGGTCCAATA CCATTGGCTC TGCGCCACCC 
CTACGGCACA GCTGGCAGAT GCCTAATGGG CACTATCGGC GGCGGAGGCG TGGGGGGCCT 
GGGCCTGGCA TGATGTGTGG AGCTGTCAGT GACCTCCTGA GTGACACGGA AGAAGACGAT 
AAGTGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017168108.1
CDS	594..7982
Translation	MVPEMRGLSPGRRLPEPTLCPATGGSPEPGAAGRRRHVLALPDEEAWRPQQCALLEANASDEVGMLPASPRSAVAGFDNFFPVQEGEGPGWEGSTTLDAGSSPFLPVSPEVMKRRRGGLIEQRDIIKAHEAHKMQSTPQARRKEWEMARFGEAVVVGRPGSGDGDSDQSRNRQGTPVPASGPAAAYKQSKAQRARTMALYNPIPVRQNCFTVNRSLFIFGEDNIVRKYAKKLIDWPPFEYMILATIIANCIVLALEQHLPEDDKTPMSRRLEKTEPYFIGIFCFEAGIKIVALGFIFHKGSYLRNGWNVMDFIVVLSGILATAGTHFNTHVDLRTLRAVRVLRPLKLVSGIPSLQIVLKSIMKAMVPLLQIGLLLFFAILMFAIIGLEFYSGKLHRACFMNNSGILEGFDPPHPCGVQGCPAGYECKDWIGPNDGITQFDNILFAVLTVFQCITMEGWTTVLYNTNDALGATWNWLYFIPLIIIGSFFVLNLVLGVLSGEFAKERERVENRRAFMKLRRQQQIERELNGYRAWIDKAEEVMLAEENKNSGTSALEVLRRATIKRSRTEAMTRDSSDEHCVDISSVGTPLARASIKSTKVDGASYFRHKERLLRISIRHMVKSQVFYWIVLSVVALNTACVAIVHHNQPQWLTHLLYYAEFLFLGLFLLEMSLKMYGMGPRLYFHSSFNCFDFGVTVGSIFEVVWAIFRPGTSFGISVLRALRLLRIFKITKYWASLRNLVVSLMSSMKSIISLLFLLFLFIVVFALLGMQLFGGRFNFNDGTPSANFDTFPAAIMTVFQILTGEDWNEVMYNGIRSQGGVSSGMWSAIYFIVLTLFGNYTLLNVFLAIAVDNLANAQELTKDEQEEEEAFNQKHALQKAKEVSPMSAPNMPSIERDRRRRHHMSMWEPRSSHLRERRRRHHMSVWEQRTSQLRRHMQMSSQEALNKEEAPPMNPLNPLNPLSPLNPLNAHPSLYRRPRPIEGLALGLGLEKCEEERISRGGSLKGDIGGLTSALDNQRSPLSLGKREPPWLPRSCHGNCDPIQQEAGGGETVVTFEDRARHRQSQRRSRHRRVRTEGKDSASASRSRSASQERSLDEGVSVEGEKEHEPHSSHRSKEPTIHEEERTQDLRRTNSLMVPRGSGLVGALDEAETPLVQPQPELEVGKDAALTEQEAEGSSEQALLGDVQLDVGRGISQSEPDLSCMTANMDKATTESTSVTVAIPDVDPLVDSTVVNISNKTDGEASPLKEAETKEEEEEVEKKKKQKKEKRETGKAMVPHSSMFIFSTTNPIRRACHYIVNLRYFEMCILLVIAASSIALAAEDPVLTNSERNKVLRYFDYVFTGVFTFEMVIKMIDQGLILQDGSYFRDLWNILDFVVVVGALVAFALANALGTNKGRDIKTIKSLRVLRVLRPLKTIKRLPKLKAVFDCVVTSLKNVFNILIVYKLFMFIFAVIAVQLFKGKFFYCTDSSKDTEKECIGNYVDHEKNKMEVKGREWKRHEFHYDNIIWALLTLFTVSTGEGWPQVLQHSVDVTEEDRGPSRSNRMEMSIFYVVYFVVFPFFFVNIFVALIIITFQEQGDKMMEECSLEKNERACIDFAISAKPLTRYMPQNRHTFQYRVWHFVVSPSFEYTIMAMIALNTVVLMMKYYSAPCTYELALKYLNIAFTMVFSLECVLKVIAFGFLNYFRDTWNIFDFITVIGSITEIILTDSKLVNTSGFNMSFLKLFRAARLIKLLRQGYTIRILLWTFVQSFKALPYVCLLIAMLFFIYAIIGMQVFGNIKLDEESHINRHNNFRSFFGSLMLLFRSATGEAWQEIMLSCLGEKGCEPDTTAPSGQNESERCGTDLAYVYFVSFIFFCSFLMLNLFVAVIMDNFEYLTRDSSILGPHHLDEFVRVWAEYDRAACGRIHYTEMYEMLTLMSPPLGLGKRCPSKVAYKRLVLMNMPVAEDMTVHFTSTLMALIRTALDIKIAKGGADRQQLDSELQKETLAIWPHLSQKMLDLLVPMPKASDLTVGKIYAAMMIMDYYKQSKVKKQRQQLEEQKNAPMFQRMEPSSLPQEIIANAKALPYLQQDPVSGLSGRSGYPSMSPLSPQEIFQLACMDPADDGQFQEQQSLEPEVSELKSVQSSNHGIYLPPDTQEHAGSGRASSMPRLTMDPQVVTDPSSMRRSFSTIRDKRSNSSWLEEFSMERSSENTYKSRRRSYHSSLRLSAHRLNSDSGHKSDTHRSGGRERGRSKERKHLLSPDVSRCNSEERGTQADWESPERRQSRSPSEGRSQTPNRQGTGSLSESSIPSISDTSTPRRSRRQLPPVPPKPRPLLSYSSLMRHTGGISPPPDGSEGGSPLASQALESNSACLTESSNSLHPQQGQHPSPQHYISEPYLALHEDSHASDCGEEETLTFEAAVATSLGRSNTIGSAPPLRHSWQMPNGHYRRRRRGGPGPGMMCGAVSDLLSDTEEDDKC

Target ORF information:

RefSeq Version	XM_017312619.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus calcium channel, voltage-dependent, R type, alpha 1E subunit (Cacna1e), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017312619.1

ORF Insert Sequence:

ATGGTGCCCG AGATGCGTGG ACTGAGCCCA GGCCGAAGGC TGCCCGAGCC CACCCTGTGC 
CCGGCTACAG GAGGGAGCCC TGAGCCCGGG GCTGCTGGCA GGCGCCGCCA CGTCCTGGCT 
CTGCCTGATG AGGAGGCATG GCGGCCGCAG CAGTGCGCCC TGCTCGAGGC CAATGCCTCC 
GACGAGGTGG GCATGTTGCC TGCTTCCCCG CGCTCCGCGG TGGCCGGCTT CGACAACTTC 
TTCCCGGTGC AGGAAGGCGA GGGCCCGGGC TGGGAGGGCA GCACCACGCT GGATGCAGGC 
TCCAGCCCCT TCCTTCCTGT GAGCCCAGAG GTCATGAAGC GGAGGCGAGG GGGGCTCATC 
GAGCAGCGGG ACATCATCAA GGCCCACGAG GCACACAAGA TGCAGAGCAC CCCGCAGGCC 
CGGCGCAAGG AGTGGGAGAT GGCTCGCTTC GGGGAGGCGG TGGTCGTTGG CAGGCCAGGC 
TCAGGCGATG GAGACTCGGA CCAGAGCAGG AACCGACAAG GAACCCCCGT CCCGGCCTCG 
GGGCCGGCGG CCGCCTACAA GCAGTCAAAA GCGCAGAGGG CGCGGACTAT GGCTTTGTAC 
AACCCCATAC CAGTCCGGCA GAACTGTTTC ACGGTCAACA GATCCCTGTT CATCTTCGGA 
GAAGATAACA TTGTCAGGAA ATACGCCAAG AAGCTCATCG ATTGGCCGCC ATTTGAGTAC 
ATGATCCTGG CCACCATCAT TGCCAACTGC ATCGTCTTGG CCCTGGAGCA GCATCTTCCT 
GAGGATGACA AGACCCCAAT GTCTCGAAGA CTGGAGAAGA CAGAACCATA TTTCATTGGG 
ATCTTCTGCT TTGAAGCTGG GATCAAAATT GTGGCTCTAG GGTTCATCTT CCATAAGGGT 
TCATACCTCC GTAATGGCTG GAATGTCATG GACTTCATCG TGGTCCTTAG TGGCATCCTG 
GCCACTGCGG GAACCCACTT CAACACCCAC GTGGACCTGC GGACCCTCCG GGCTGTGCGT 
GTCCTGAGGC CTTTAAAGCT GGTATCAGGA ATACCTAGCC TGCAGATCGT GCTGAAGTCT 
ATCATGAAGG CCATGGTGCC TCTTCTGCAG ATTGGCCTTC TGCTCTTCTT TGCCATCCTC 
ATGTTTGCTA TCATTGGCTT GGAGTTCTAC AGTGGCAAGT TGCATCGAGC ATGCTTCATG 
AACAATTCAG GTATTCTAGA GGGGTTTGAT CCTCCTCACC CGTGTGGTGT GCAAGGCTGC 
CCGGCTGGTT ATGAATGTAA GGACTGGATC GGCCCCAATG ACGGGATCAC CCAGTTTGAC 
AACATCCTTT TTGCCGTGCT GACTGTCTTC CAATGCATCA CTATGGAAGG GTGGACCACT 
GTGCTGTACA ATACCAATGA TGCCTTAGGA GCCACCTGGA ACTGGCTGTA CTTCATCCCT 
CTCATCATCA TCGGATCCTT CTTTGTTCTC AACCTCGTCC TGGGAGTGCT TTCCGGGGAA 
TTTGCCAAAG AAAGAGAGAG AGTGGAGAAC CGAAGAGCGT TCATGAAGCT CCGGCGCCAG 
CAACAGATTG AGCGAGAATT GAACGGCTAC CGTGCCTGGA TAGACAAAGC AGAGGAAGTC 
ATGCTTGCTG AAGAAAATAA AAACTCGGGA ACATCAGCCT TGGAAGTGCT TCGAAGGGCA 
ACCATCAAAA GGAGCCGGAC GGAGGCCATG ACCCGAGACT CCAGCGATGA GCACTGCGTT 
GATATCTCTT CAGTGGGCAC ACCTCTTGCC AGAGCCAGTA TCAAGAGCAC AAAAGTAGAT 
GGGGCCTCCT ATTTCCGGCA CAAGGAACGG CTTCTGCGCA TCTCTATCCG CCACATGGTC 
AAGTCCCAAG TCTTTTACTG GATCGTCCTG AGTGTTGTGG CTCTTAATAC TGCCTGTGTG 
GCCATTGTTC ATCACAACCA GCCACAGTGG CTCACTCACC TCCTCTACTA TGCAGAGTTT 
TTGTTTCTGG GACTCTTCCT GTTGGAGATG TCCCTGAAGA TGTATGGCAT GGGGCCACGC 
CTCTATTTTC ACTCATCCTT CAACTGCTTT GATTTTGGGG TCACAGTGGG CAGCATCTTT 
GAGGTTGTCT GGGCAATCTT CAGACCTGGC ACATCTTTTG GAATCAGTGT CTTACGAGCT 
CTCCGGCTCC TAAGGATATT TAAAATAACC AAGTATTGGG CATCCCTCCG GAATCTGGTT 
GTCTCCTTGA TGAGCTCCAT GAAGTCTATC ATCAGCTTAC TCTTCCTTCT CTTCCTCTTC 
ATCGTTGTCT TTGCTCTCCT GGGAATGCAG CTCTTTGGAG GCAGGTTTAA CTTTAATGAT 
GGGACTCCTT CAGCTAATTT TGACACCTTC CCTGCAGCTA TCATGACAGT GTTCCAGATC 
CTGACAGGGG AAGACTGGAA TGAGGTGATG TACAATGGCA TTCGCTCCCA GGGTGGGGTC 
AGCTCAGGCA TGTGGTCTGC CATCTACTTC ATTGTGCTCA CCTTGTTTGG AAACTACACG 
CTGCTAAATG TATTCTTGGC CATCGCTGTG GACAATCTGG CCAATGCCCA GGAACTGACC 
AAGGATGAGC AGGAGGAAGA AGAAGCCTTC AACCAGAAAC ATGCACTGCA GAAGGCCAAG 
GAGGTCAGCC CGATGTCTGC TCCCAACATG CCTTCCATTG AAAGAGACAG AAGGAGAAGA 
CACCACATGT CGATGTGGGA GCCACGCAGC AGCCACCTGA GGGAGCGGAG GCGCCGGCAC 
CACATGTCTG TGTGGGAACA ACGCACCAGC CAGCTGAGGA GGCACATGCA AATGTCTAGC 
CAAGAAGCCC TCAACAAAGA GGAGGCCCCG CCAATGAACC CCCTCAATCC ACTCAACCCA 
CTGAGTCCTC TCAACCCCCT CAATGCTCAC CCCAGCCTTT ATCGGAGGCC CAGGCCCATT 
GAGGGCCTAG CCCTAGGTCT GGGTCTTGAA AAGTGTGAAG AAGAACGCAT CAGTCGTGGG 
GGCTCCCTCA AGGGGGACAT AGGGGGCCTG ACTAGCGCCC TGGACAACCA GAGGAGCCCC 
TTGTCCTTGG GCAAACGGGA GCCACCGTGG CTGCCTAGAT CCTGTCATGG AAACTGTGAC 
CCAATCCAGC AGGAGGCTGG GGGAGGAGAG ACTGTGGTGA CTTTTGAGGA CCGGGCCAGG 
CACAGGCAGA GTCAGAGGCG AAGCCGGCAT CGCCGAGTCA GGACTGAGGG CAAGGACTCT 
GCCTCTGCAT CCCGAAGCAG GTCTGCCAGC CAGGAGCGGA GTCTGGATGA AGGGGTGTCC 
GTTGAGGGGG AGAAGGAACA TGAGCCTCAC AGCAGCCACA GGAGCAAAGA GCCGACCATC 
CACGAAGAGG AGAGAACCCA GGACTTAAGG AGGACCAACA GTCTGATGGT CCCCAGGGGC 
TCTGGACTGG TAGGGGCTCT TGATGAAGCA GAAACACCTC TAGTCCAGCC CCAACCTGAG 
CTGGAAGTGG GGAAGGATGC AGCTCTGACA GAGCAGGAGG CTGAAGGCAG CAGTGAACAA 
GCCCTTCTTG GGGACGTACA GCTGGATGTG GGCCGGGGCA TTAGCCAGAG TGAACCGGAT 
CTCTCCTGCA TGACAGCCAA CATGGACAAG GCTACCACGG AGAGCACCAG TGTCACGGTC 
GCCATCCCTG ATGTGGACCC CTTGGTGGAC TCGACAGTGG TGAACATTAG CAACAAGACG 
GATGGAGAAG CCAGTCCCTT GAAGGAGGCA GAGACTAAAG AGGAAGAAGA AGAGGTAGAG 
AAGAAGAAGA AGCAGAAGAA GGAGAAGCGA GAGACTGGAA AAGCCATGGT GCCCCATAGC 
TCTATGTTCA TCTTCAGCAC CACTAACCCG ATCCGCAGGG CCTGCCACTA CATTGTGAAC 
CTACGCTACT TTGAAATGTG CATTCTCTTG GTGATTGCGG CCAGCAGCAT TGCCCTGGCA 
GCCGAGGACC CTGTACTAAC TAACTCGGAG CGTAACAAAG TCCTGAGATA TTTCGACTAT 
GTTTTCACAG GAGTGTTTAC CTTTGAGATG GTTATAAAGA TGATAGACCA AGGCTTGATC 
CTGCAGGATG GATCATACTT CAGAGACCTC TGGAACATCC TGGACTTTGT AGTAGTGGTT 
GGTGCATTGG TAGCATTTGC TCTGGCGAAC GCTTTGGGAA CCAACAAGGG GAGGGATATC 
AAGACTATCA AGTCTCTGAG GGTACTTCGA GTTCTGCGGC CACTGAAAAC CATCAAGCGC 
TTGCCCAAGC TGAAGGCTGT GTTTGACTGC GTGGTGACCT CCTTGAAGAA TGTCTTCAAC 
ATACTCATTG TCTACAAGCT CTTCATGTTC ATCTTTGCCG TCATCGCTGT TCAGCTATTC 
AAAGGAAAGT TCTTTTATTG CACTGACAGT TCCAAGGACA CAGAGAAGGA GTGCATAGGC 
AACTATGTAG ACCACGAGAA AAACAAGATG GAGGTGAAGG GCCGGGAATG GAAGCGTCAT 
GAATTCCACT ATGACAACAT CATCTGGGCC CTGCTTACCC TCTTCACAGT CTCTACAGGG 
GAAGGATGGC CTCAGGTTCT GCAGCACTCT GTAGATGTGA CAGAGGAGGA CAGAGGCCCA 
AGTCGCAGCA ACCGCATGGA GATGTCCATC TTTTATGTGG TCTACTTTGT GGTCTTCCCT 
TTCTTCTTTG TCAATATCTT CGTGGCTCTC ATTATCATCA CCTTCCAGGA GCAAGGGGAC 
AAGATGATGG AGGAGTGTAG CCTGGAGAAG AATGAGCGGG CATGCATCGA CTTCGCCATT 
AGTGCCAAAC CTCTCACCCG CTACATGCCA CAGAACAGGC ACACCTTCCA GTACCGTGTG 
TGGCACTTTG TAGTCTCCCC ATCCTTTGAG TATACCATCA TGGCCATGAT TGCCTTGAAC 
ACTGTCGTGC TGATGATGAA GTACTACTCT GCTCCTTGCA CCTATGAGCT CGCCTTAAAG 
TACCTGAACA TCGCCTTCAC TATGGTGTTT TCCCTGGAGT GTGTCCTAAA GGTCATTGCT 
TTCGGCTTCC TGAACTATTT CCGAGATACC TGGAACATCT TTGACTTCAT CACGGTGATC 
GGCAGTATCA CAGAAATTAT CCTGACAGAT AGCAAGCTGG TGAACACCAG TGGCTTCAAT 
ATGAGCTTTC TGAAGCTCTT CCGAGCTGCA CGGCTCATAA AGCTTCTGAG ACAGGGCTAC 
ACCATCCGCA TCTTACTGTG GACCTTCGTG CAGTCCTTTA AGGCTCTTCC CTACGTCTGC 
CTTCTCATTG CCATGCTTTT CTTCATCTAC GCCATCATTG GGATGCAGGT GTTTGGAAAC 
ATAAAGTTAG ATGAGGAGAG TCACATCAAC CGGCACAACA ACTTCCGGAG TTTCTTTGGA 
TCCCTCATGC TGCTTTTCAG GAGTGCCACG GGGGAGGCTT GGCAGGAGAT TATGCTGTCC 
TGTCTCGGTG AGAAGGGTTG TGAGCCTGAC ACCACTGCAC CCTCAGGGCA GAATGAGAGT 
GAGCGCTGTG GCACTGACCT GGCCTACGTT TATTTTGTCT CCTTCATCTT CTTCTGCTCC 
TTCCTGATGC TCAACCTGTT CGTGGCTGTC ATCATGGACA ACTTTGAGTA CCTGACTCGA 
GATTCCTCCA TCCTGGGGCC TCACCACTTA GATGAGTTTG TCCGTGTCTG GGCAGAATAT 
GACAGAGCAG CATGTGGCCG CATCCATTAC ACTGAGATGT ATGAAATGCT GACTCTCATG 
TCACCACCGC TAGGCCTCGG CAAGAGATGT CCCTCCAAAG TGGCCTATAA GAGGCTGGTC 
CTGATGAACA TGCCAGTGGC TGAGGACATG ACAGTCCACT TCACCTCCAC ACTTATGGCT 
CTGATCCGGA CAGCTCTGGA CATTAAAATC GCCAAAGGTG GTGCAGACAG ACAGCAGCTA 
GACTCGGAGC TGCAAAAAGA GACCCTGGCC ATTTGGCCTC ACCTGTCCCA AAAGATGTTG 
GATCTGCTTG TGCCCATGCC CAAAGCCTCT GACCTGACTG TGGGCAAGAT CTATGCAGCA 
ATGATGATCA TGGACTACTA TAAACAGAGT AAGGTAAAGA AACAGAGACA GCAGCTGGAA 
GAACAGAAAA ATGCACCCAT GTTCCAGCGC ATGGAGCCCT CATCACTGCC TCAGGAGATC 
ATTGCTAATG CCAAAGCCCT GCCTTATCTC CAGCAGGACC CTGTTTCTGG CCTGAGTGGT 
CGGAGTGGAT ACCCTTCAAT GAGTCCACTC TCCCCTCAAG AAATATTCCA GTTGGCTTGT 
ATGGACCCAG CGGATGATGG ACAATTCCAG GAACAACAAT CTCTGGAGCC AGAGGTTAGT 
GAATTAAAAA GTGTGCAGTC CTCTAACCAT GGCATCTACC TTCCTCCGGA CACCCAGGAG 
CATGCGGGAT CTGGGAGGGC ATCCTCTATG CCACGTCTGA CTATGGATCC CCAGGTGGTA 
ACAGACCCTA GCTCTATGCG ACGTTCATTT TCTACAATTC GGGATAAGCG TTCAAATTCC 
TCCTGGTTGG AGGAGTTCTC CATGGAACGC AGCAGTGAAA ACACTTACAA GTCGCGGCGC 
AGAAGTTACC ATTCCTCCCT GAGGCTGTCA GCACACCGCC TGAATTCTGA CTCTGGTCAC 
AAGTCTGACA CTCACCGATC AGGAGGCAGG GAGAGAGGCC GATCCAAAGA ACGAAAGCAC 
CTCCTCTCTC CAGATGTCTC CCGCTGCAAT TCTGAAGAGC GAGGGACCCA GGCCGACTGG 
GAGTCCCCAG AACGTCGCCA GTCCAGGTCA CCCAGTGAAG GAAGGTCACA GACCCCCAAT 
AGACAGGGTA CCGGTTCCCT AAGTGAGAGC TCCATCCCCT CTATTTCCGA CACCAGCACC 
CCAAGACGAA GCCGTCGCCA GCTCCCACCT GTGCCACCAA AGCCTCGTCC CCTCCTCTCC 
TACAGCTCCC TGATGAGACA CACTGGCGGC ATCTCTCCAC CTCCTGATGG AAGTGAGGGT 
GGATCCCCAC TGGCCTCTCA AGCTCTGGAG AGCAACAGCG CTTGCCTGAC CGAGTCTTCC 
AACTCCTTGC ACCCCCAGCA GGGCCAGCAT CCTTCCCCAC AGCACTACAT CTCCGAGCCC 
TATCTGGCCC TCCATGAAGA CTCCCACGCC TCAGACTGTG GCGAGGAGGA GACACTCACC 
TTTGAGGCAG CCGTAGCCAC TAGCTTGGGC CGGTCCAATA CCATTGGCTC TGCGCCACCC 
CTACGGCACA GCTGGCAGAT GCCTAATGGG CACTATCGGC GGCGGAGGCG TGGGGGGCCT 
GGGCCTGGCA TGATGTGTGG AGCTGTCAGT GACCTCCTGA GTGACACGGA AGAAGACGAT 
AAGTGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cacna1e ( XM_017312682.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017168171.1 Mus musculus calcium channel, voltage-dependent, R type, alpha 1E subunit (Cacna1e), transcript variant X10, mRNA.

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CloneID	OMu27874
Clone ID Related Accession (Same CDS sequence)	XM_017312682.1
Accession Version	XM_017312682.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7332bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	voltage-dependent R-type calcium channel subunit alpha-1E isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGCCCG AGATGCGTGG ACTGAGCCCA GGCCGAAGGC TGCCCGAGCC CACCCTGTGC 
CCGGCTACAG GAGGGAGCCC TGAGCCCGGG GCTGCTGGCA GGCGCCGCCA CGTCCTGGCT 
CTGCCTGATG AGGAGGCATG GCGGCCGCAG CAGTGCGCCC TGCTCGAGGC CAATGCCTCC 
GACGAGGTGG GCATGTTGCC TGCTTCCCCG CGCTCCGCGG TGGCCGGCTT CGACAACTTC 
TTCCCGGTGC AGGAAGGCGA GGGCCCGGGC TGGGAGGGCA GCACCACGCT GGATGCAGGC 
TCCAGCCCCT TCCTTCCTGT GAGCCCAGAG GTCATGAAGC GGAGGCGAGG GGGGCTCATC 
GAGCAGCGGG ACATCATCAA GGCCCACGAG GCACACAAGA TGCAGAGCAC CCCGCAGGCC 
CGGCGCAAGG AGTGGGAGAT GGCTCGCTTC GGGGAGGCGG TGGTCGTTGG CAGGCCAGGC 
TCAGGCGATG GAGACTCGGA CCAGAGCAGG AACCGACAAG GAACCCCCGT CCCGGCCTCG 
GGGCCGGCGG CCGCCTACAA GCAGTCAAAA GCGCAGAGGG CGCGGACTAT GGCTTTGTAC 
AACCCCATAC CAGTCCGGCA GAACTGTTTC ACGGTCAACA GATCCCTGTT CATCTTCGGA 
GAAGATAACA TTGTCAGGAA ATACGCCAAG AAGCTCATCG ATTGGCCGCC ATTTGAGTAC 
ATGATCCTGG CCACCATCAT TGCCAACTGC ATCGTCTTGG CCCTGGAGCA GCATCTTCCT 
GAGGATGACA AGACCCCAAT GTCTCGAAGA CTGGAGAAGA CAGAACCATA TTTCATTGGG 
ATCTTCTGCT TTGAAGCTGG GATCAAAATT GTGGCTCTAG GGTTCATCTT CCATAAGGGT 
TCATACCTCC GTAATGGCTG GAATGTCATG GACTTCATCG TGGTCCTTAG TGGCATCCTG 
GCCACTGCGG GAACCCACTT CAACACCCAC GTGGACCTGC GGACCCTCCG GGCTGTGCGT 
GTCCTGAGGC CTTTAAAGCT GGTATCAGGA ATACCTAGCC TGCAGATCGT GCTGAAGTCT 
ATCATGAAGG CCATGGTGCC TCTTCTGCAG ATTGGCCTTC TGCTCTTCTT TGCCATCCTC 
ATGTTTGCTA TCATTGGCTT GGAGTTCTAC AGTGGCAAGT TGCATCGAGC ATGCTTCATG 
AACAATTCAG GTATTCTAGA GGGGTTTGAT CCTCCTCACC CGTGTGGTGT GCAAGGCTGC 
CCGGCTGGTT ATGAATGTAA GGACTGGATC GGCCCCAATG ACGGGATCAC CCAGTTTGAC 
AACATCCTTT TTGCCGTGCT GACTGTCTTC CAATGCATCA CTATGGAAGG GTGGACCACT 
GTGCTGTACA ATACCAATGA TGCCTTAGGA GCCACCTGGA ACTGGCTGTA CTTCATCCCT 
CTCATCATCA TCGGATCCTT CTTTGTTCTC AACCTCGTCC TGGGAGTGCT TTCCGGGGAA 
TTTGCCAAAG AAAGAGAGAG AGTGGAGAAC CGAAGAGCGT TCATGAAGCT CCGGCGCCAG 
CAACAGATTG AGCGAGAATT GAACGGCTAC CGTGCCTGGA TAGACAAAGC AGAGGAAGTC 
ATGCTTGCTG AAGAAAATAA AAACTCGGGA ACATCAGCCT TGGAAGTGCT TCGAAGGGCA 
ACCATCAAAA GGAGCCGGAC GGAGGCCATG ACCCGAGACT CCAGCGATGA GCACTGCGTT 
GATATCTCTT CAGTGGGCAC ACCTCTTGCC AGAGCCAGTA TCAAGAGCAC AAAAGTAGAT 
GGGGCCTCCT ATTTCCGGCA CAAGGAACGG CTTCTGCGCA TCTCTATCCG CCACATGGTC 
AAGTCCCAAG TCTTTTACTG GATCGTCCTG AGTGTTGTGG CTCTTAATAC TGCCTGTGTG 
GCCATTGTTC ATCACAACCA GCCACAGTGG CTCACTCACC TCCTCTACTA TGCAGAGTTT 
TTGTTTCTGG GACTCTTCCT GTTGGAGATG TCCCTGAAGA TGTATGGCAT GGGGCCACGC 
CTCTATTTTC ACTCATCCTT CAACTGCTTT GATTTTGGGG TCACAGTGGG CAGCATCTTT 
GAGGTTGTCT GGGCAATCTT CAGACCTGGC ACATCTTTTG GAATCAGTGT CTTACGAGCT 
CTCCGGCTCC TAAGGATATT TAAAATAACC AAGTATTGGG CATCCCTCCG GAATCTGGTT 
GTCTCCTTGA TGAGCTCCAT GAAGTCTATC ATCAGCTTAC TCTTCCTTCT CTTCCTCTTC 
ATCGTTGTCT TTGCTCTCCT GGGAATGCAG CTCTTTGGAG GCAGGTTTAA CTTTAATGAT 
GGGACTCCTT CAGCTAATTT TGACACCTTC CCTGCAGCTA TCATGACAGT GTTCCAGATC 
CTGACAGGGG AAGACTGGAA TGAGGTGATG TACAATGGCA TTCGCTCCCA GGGTGGGGTC 
AGCTCAGGCA TGTGGTCTGC CATCTACTTC ATTGTGCTCA CCTTGTTTGG AAACTACACG 
CTGCTAAATG TATTCTTGGC CATCGCTGTG GACAATCTGG CCAATGCCCA GGAACTGACC 
AAGGATGAGC AGGAGGAAGA AGAAGCCTTC AACCAGAAAC ATGCACTGCA GAAGGCCAAG 
GAGGTCAGCC CGATGTCTGC TCCCAACATG CCTTCCATTG AGAGGGAGCG GAGGCGCCGG 
CACCACATGT CTGTGTGGGA ACAACGCACC AGCCAGCTGA GGAGGCACAT GCAAATGTCT 
AGCCAAGAAG CCCTCAACAA AGAGGAGGCC CCGCCAATGA ACCCCCTCAA TCCACTCAAC 
CCACTGAGTC CTCTCAACCC CCTCAATGCT CACCCCAGCC TTTATCGGAG GCCCAGGCCC 
ATTGAGGGCC TAGCCCTAGG TCTGGGTCTT GAAAAGTGTG AAGAAGAACG CATCAGTCGT 
GGGGGCTCCC TCAAGGGGGA CATAGGGGGC CTGACTAGCG CCCTGGACAA CCAGAGGAGC 
CCCTTGTCCT TGGGCAAACG GGAGCCACCG TGGCTGCCTA GATCCTGTCA TGGAAACTGT 
GACCCAATCC AGCAGGAGGC TGGGGGAGGA GAGACTGTGG TGACTTTTGA GGACCGGGCC 
AGGCACAGGC AGAGTCAGAG GCGAAGCCGG CATCGCCGAG TCAGGACTGA GGGCAAGGAC 
TCTGCCTCTG CATCCCGAAG CAGGTCTGCC AGCCAGGAGC GGAGTCTGGA TGAAGGGGTG 
TCCGTTGAGG GGGAGAAGGA ACATGAGCCT CACAGCAGCC ACAGGAGCAA AGAGCCGACC 
ATCCACGAAG AGGAGAGAAC CCAGGACTTA AGGAGGACCA ACAGTCTGAT GGTCCCCAGG 
GGCTCTGGAC TGGTAGGGGC TCTTGATGAA GCAGAAACAC CTCTAGTCCA GCCCCAACCT 
GAGCTGGAAG TGGGGAAGGA TGCAGCTCTG ACAGAGCAGG AGGCTGAAGG CAGCAGTGAA 
CAAGCCCTTC TTGGGGACGT ACAGCTGGAT GTGGGCCGGG GCATTAGCCA GAGTGAACCG 
GATCTCTCCT GCATGACAGC CAACATGGAC AAGGCTACCA CGGAGAGCAC CAGTGTCACG 
GTCGCCATCC CTGATGTGGA CCCCTTGGTG GACTCGACAG TGGTGAACAT TAGCAACAAG 
ACGGATGGAG AAGCCAGTCC CTTGAAGGAG GCAGAGACTA AAGAGGAAGA AGAAGAGGTA 
GAGAAGAAGA AGAAGCAGAA GAAGGAGAAG CGAGAGACTG GAAAAGCCAT GGTGCCCCAT 
AGCTCTATGT TCATCTTCAG CACCACTAAC CCGATCCGCA GGGCCTGCCA CTACATTGTG 
AACCTACGCT ACTTTGAAAT GTGCATTCTC TTGGTGATTG CGGCCAGCAG CATTGCCCTG 
GCAGCCGAGG ACCCTGTACT AACTAACTCG GAGCGTAACA AAGTCCTGAG ATATTTCGAC 
TATGTTTTCA CAGGAGTGTT TACCTTTGAG ATGGTTATAA AGATGATAGA CCAAGGCTTG 
ATCCTGCAGG ATGGATCATA CTTCAGAGAC CTCTGGAACA TCCTGGACTT TGTAGTAGTG 
GTTGGTGCAT TGGTAGCATT TGCTCTGGCG AACGCTTTGG GAACCAACAA GGGGAGGGAT 
ATCAAGACTA TCAAGTCTCT GAGGGTACTT CGAGTTCTGC GGCCACTGAA AACCATCAAG 
CGCTTGCCCA AGCTGAAGGC TGTGTTTGAC TGCGTGGTGA CCTCCTTGAA GAATGTCTTC 
AACATACTCA TTGTCTACAA GCTCTTCATG TTCATCTTTG CCGTCATCGC TGTTCAGCTA 
TTCAAAGGAA AGTTCTTTTA TTGCACTGAC AGTTCCAAGG ACACAGAGAA GGAGTGCATA 
GGCAACTATG TAGACCACGA GAAAAACAAG ATGGAGGTGA AGGGCCGGGA ATGGAAGCGT 
CATGAATTCC ACTATGACAA CATCATCTGG GCCCTGCTTA CCCTCTTCAC AGTCTCTACA 
GGGGAAGGAT GGCCTCAGGT TCTGCAGCAC TCTGTAGATG TGACAGAGGA GGACAGAGGC 
CCAAGTCGCA GCAACCGCAT GGAGATGTCC ATCTTTTATG TGGTCTACTT TGTGGTCTTC 
CCTTTCTTCT TTGTCAATAT CTTCGTGGCT CTCATTATCA TCACCTTCCA GGAGCAAGGG 
GACAAGATGA TGGAGGAGTG TAGCCTGGAG AAGAATGAGC GGGCATGCAT CGACTTCGCC 
ATTAGTGCCA AACCTCTCAC CCGCTACATG CCACAGAACA GGCACACCTT CCAGTACCGT 
GTGTGGCACT TTGTAGTCTC CCCATCCTTT GAGTATACCA TCATGGCCAT GATTGCCTTG 
AACACTGTCG TGCTGATGAT GAAGTACTAC TCTGCTCCTT GCACCTATGA GCTCGCCTTA 
AAGTACCTGA ACATCGCCTT CACTATGGTG TTTTCCCTGG AGTGTGTCCT AAAGGTCATT 
GCTTTCGGCT TCCTGAACTA TTTCCGAGAT ACCTGGAACA TCTTTGACTT CATCACGGTG 
ATCGGCAGTA TCACAGAAAT TATCCTGACA GATAGCAAGC TGGTGAACAC CAGTGGCTTC 
AATATGAGCT TTCTGAAGCT CTTCCGAGCT GCACGGCTCA TAAAGCTTCT GAGACAGGGC 
TACACCATCC GCATCTTACT GTGGACCTTC GTGCAGTCCT TTAAGGCTCT TCCCTACGTC 
TGCCTTCTCA TTGCCATGCT TTTCTTCATC TACGCCATCA TTGGGATGCA GGTGTTTGGA 
AACATAAAGT TAGATGAGGA GAGTCACATC AACCGGCACA ACAACTTCCG GAGTTTCTTT 
GGATCCCTCA TGCTGCTTTT CAGGAGTGCC ACGGGGGAGG CTTGGCAGGA GATTATGCTG 
TCCTGTCTCG GTGAGAAGGG TTGTGAGCCT GACACCACTG CACCCTCAGG GCAGAATGAG 
AGTGAGCGCT GTGGCACTGA CCTGGCCTAC GTTTATTTTG TCTCCTTCAT CTTCTTCTGC 
TCCTTCCTGA TGCTCAACCT GTTCGTGGCT GTCATCATGG ACAACTTTGA GTACCTGACT 
CGAGATTCCT CCATCCTGGG GCCTCACCAC TTAGATGAGT TTGTCCGTGT CTGGGCAGAA 
TATGACAGAG CAGCATGTGG CCGCATCCAT TACACTGAGA TGTATGAAAT GCTGACTCTC 
ATGTCACCAC CGCTAGGCCT CGGCAAGAGA TGTCCCTCCA AAGTGGCCTA TAAGAGGCTG 
GTCCTGATGA ACATGCCAGT GGCTGAGGAC ATGACAGTCC ACTTCACCTC CACACTTATG 
GCTCTGATCC GGACAGCTCT GGACATTAAA ATCGCCAAAG GTGGTGCAGA CAGACAGCAG 
CTAGACTCGG AGCTGCAAAA AGAGACCCTG GCCATTTGGC CTCACCTGTC CCAAAAGATG 
TTGGATCTGC TTGTGCCCAT GCCCAAAGCC TCTGACCTGA CTGTGGGCAA GATCTATGCA 
GCAATGATGA TCATGGACTA CTATAAACAG AGTAAGGTAA AGAAACAGAG ACAGCAGCTG 
GAAGAACAGA AAAATGCACC CATGTTCCAG CGCATGGAGC CCTCATCACT GCCTCAGGAG 
ATCATTGCTA ATGCCAAAGC CCTGCCTTAT CTCCAGCAGG ACCCTGTTTC TGGCCTGAGT 
GGTCGGAGTG GATACCCTTC AATGAGTCCA CTCTCCCCTC AAGAAATATT CCAGTTGGCT 
TGTATGGACC CAGCGGATGA TGGACAATTC CAGGAACAAC AATCTCTGGA GCCAGAGGTT 
AGTGAATTAA AAAGTGTGCA GTCCTCTAAC CATGGCATCT ACCTTCCTCC GGACACCCAG 
GAGCATGCGG GATCTGGGAG GGCATCCTCT ATGCCACGTC TGACTATGGA TCCCCAGGTG 
GTAACAGACC CTAGCTCTAT GCGACGTTCA TTTTCTACAA TTCGGGATAA GCGTTCAAAT 
TCCTCCTGGT TGGAGGAGTT CTCCATGGAA CGCAGCAGTG AAAACACTTA CAAGTCGCGG 
CGCAGAAGTT ACCATTCCTC CCTGAGGCTG TCAGCACACC GCCTGAATTC TGACTCTGGT 
CACAAGTCTG ACACTCACCG ATCAGGAGGC AGGGAGAGAG GCCGATCCAA AGAACGAAAG 
CACCTCCTCT CTCCAGATGT CTCCCGCTGC AATTCTGAAG AGCGAGGGAC CCAGGCCGAC 
TGGGAGTCCC CAGAACGTCG CCAGTCCAGG TCACCCAGTG AAGGAAGGTC ACAGACCCCC 
AATAGACAGG GTACCGGTTC CCTAAGTGAG AGCTCCATCC CCTCTATTTC CGACACCAGC 
ACCCCAAGAC GAAGCCGTCG CCAGCTCCCA CCTGTGCCAC CAAAGCCTCG TCCCCTCCTC 
TCCTACAGCT CCCTGATGAG ACACACTGGC GGCATCTCTC CACCTCCTGA TGGAAGTGAG 
GGTGGATCCC CACTGGCCTC TCAAGCTCTG GAGAGCAACA GCGCTTGCCT GACCGAGTCT 
TCCAACTCCT TGCACCCCCA GCAGGGCCAG CATCCTTCCC CACAGCACTA CATCTCCGAG 
CCCTATCTGG CCCTCCATGA AGACTCCCAC GCCTCAGACT GTGGCGAGGA GGAGACACTC 
ACCTTTGAGG CAGCCGTAGC CACTAGCTTG GGCCGGTCCA ATACCATTGG CTCTGCGCCA 
CCCCTACGGC ACAGCTGGCA GATGCCTAAT GGGCACTATC GGCGGCGGAG GCGTGGGGGG 
CCTGGGCCTG GCATGATGTG TGGAGCTGTC AGTGACCTCC TGAGTGACAC GGAAGAAGAC 
GATAAGTGCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017168171.1
CDS	594..7925
Translation	MVPEMRGLSPGRRLPEPTLCPATGGSPEPGAAGRRRHVLALPDEEAWRPQQCALLEANASDEVGMLPASPRSAVAGFDNFFPVQEGEGPGWEGSTTLDAGSSPFLPVSPEVMKRRRGGLIEQRDIIKAHEAHKMQSTPQARRKEWEMARFGEAVVVGRPGSGDGDSDQSRNRQGTPVPASGPAAAYKQSKAQRARTMALYNPIPVRQNCFTVNRSLFIFGEDNIVRKYAKKLIDWPPFEYMILATIIANCIVLALEQHLPEDDKTPMSRRLEKTEPYFIGIFCFEAGIKIVALGFIFHKGSYLRNGWNVMDFIVVLSGILATAGTHFNTHVDLRTLRAVRVLRPLKLVSGIPSLQIVLKSIMKAMVPLLQIGLLLFFAILMFAIIGLEFYSGKLHRACFMNNSGILEGFDPPHPCGVQGCPAGYECKDWIGPNDGITQFDNILFAVLTVFQCITMEGWTTVLYNTNDALGATWNWLYFIPLIIIGSFFVLNLVLGVLSGEFAKERERVENRRAFMKLRRQQQIERELNGYRAWIDKAEEVMLAEENKNSGTSALEVLRRATIKRSRTEAMTRDSSDEHCVDISSVGTPLARASIKSTKVDGASYFRHKERLLRISIRHMVKSQVFYWIVLSVVALNTACVAIVHHNQPQWLTHLLYYAEFLFLGLFLLEMSLKMYGMGPRLYFHSSFNCFDFGVTVGSIFEVVWAIFRPGTSFGISVLRALRLLRIFKITKYWASLRNLVVSLMSSMKSIISLLFLLFLFIVVFALLGMQLFGGRFNFNDGTPSANFDTFPAAIMTVFQILTGEDWNEVMYNGIRSQGGVSSGMWSAIYFIVLTLFGNYTLLNVFLAIAVDNLANAQELTKDEQEEEEAFNQKHALQKAKEVSPMSAPNMPSIERERRRRHHMSVWEQRTSQLRRHMQMSSQEALNKEEAPPMNPLNPLNPLSPLNPLNAHPSLYRRPRPIEGLALGLGLEKCEEERISRGGSLKGDIGGLTSALDNQRSPLSLGKREPPWLPRSCHGNCDPIQQEAGGGETVVTFEDRARHRQSQRRSRHRRVRTEGKDSASASRSRSASQERSLDEGVSVEGEKEHEPHSSHRSKEPTIHEEERTQDLRRTNSLMVPRGSGLVGALDEAETPLVQPQPELEVGKDAALTEQEAEGSSEQALLGDVQLDVGRGISQSEPDLSCMTANMDKATTESTSVTVAIPDVDPLVDSTVVNISNKTDGEASPLKEAETKEEEEEVEKKKKQKKEKRETGKAMVPHSSMFIFSTTNPIRRACHYIVNLRYFEMCILLVIAASSIALAAEDPVLTNSERNKVLRYFDYVFTGVFTFEMVIKMIDQGLILQDGSYFRDLWNILDFVVVVGALVAFALANALGTNKGRDIKTIKSLRVLRVLRPLKTIKRLPKLKAVFDCVVTSLKNVFNILIVYKLFMFIFAVIAVQLFKGKFFYCTDSSKDTEKECIGNYVDHEKNKMEVKGREWKRHEFHYDNIIWALLTLFTVSTGEGWPQVLQHSVDVTEEDRGPSRSNRMEMSIFYVVYFVVFPFFFVNIFVALIIITFQEQGDKMMEECSLEKNERACIDFAISAKPLTRYMPQNRHTFQYRVWHFVVSPSFEYTIMAMIALNTVVLMMKYYSAPCTYELALKYLNIAFTMVFSLECVLKVIAFGFLNYFRDTWNIFDFITVIGSITEIILTDSKLVNTSGFNMSFLKLFRAARLIKLLRQGYTIRILLWTFVQSFKALPYVCLLIAMLFFIYAIIGMQVFGNIKLDEESHINRHNNFRSFFGSLMLLFRSATGEAWQEIMLSCLGEKGCEPDTTAPSGQNESERCGTDLAYVYFVSFIFFCSFLMLNLFVAVIMDNFEYLTRDSSILGPHHLDEFVRVWAEYDRAACGRIHYTEMYEMLTLMSPPLGLGKRCPSKVAYKRLVLMNMPVAEDMTVHFTSTLMALIRTALDIKIAKGGADRQQLDSELQKETLAIWPHLSQKMLDLLVPMPKASDLTVGKIYAAMMIMDYYKQSKVKKQRQQLEEQKNAPMFQRMEPSSLPQEIIANAKALPYLQQDPVSGLSGRSGYPSMSPLSPQEIFQLACMDPADDGQFQEQQSLEPEVSELKSVQSSNHGIYLPPDTQEHAGSGRASSMPRLTMDPQVVTDPSSMRRSFSTIRDKRSNSSWLEEFSMERSSENTYKSRRRSYHSSLRLSAHRLNSDSGHKSDTHRSGGRERGRSKERKHLLSPDVSRCNSEERGTQADWESPERRQSRSPSEGRSQTPNRQGTGSLSESSIPSISDTSTPRRSRRQLPPVPPKPRPLLSYSSLMRHTGGISPPPDGSEGGSPLASQALESNSACLTESSNSLHPQQGQHPSPQHYISEPYLALHEDSHASDCGEEETLTFEAAVATSLGRSNTIGSAPPLRHSWQMPNGHYRRRRRGGPGPGMMCGAVSDLLSDTEEDDKC

Target ORF information:

RefSeq Version	XM_017312682.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus calcium channel, voltage-dependent, R type, alpha 1E subunit (Cacna1e), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017312682.1

ORF Insert Sequence:

ATGGTGCCCG AGATGCGTGG ACTGAGCCCA GGCCGAAGGC TGCCCGAGCC CACCCTGTGC 
CCGGCTACAG GAGGGAGCCC TGAGCCCGGG GCTGCTGGCA GGCGCCGCCA CGTCCTGGCT 
CTGCCTGATG AGGAGGCATG GCGGCCGCAG CAGTGCGCCC TGCTCGAGGC CAATGCCTCC 
GACGAGGTGG GCATGTTGCC TGCTTCCCCG CGCTCCGCGG TGGCCGGCTT CGACAACTTC 
TTCCCGGTGC AGGAAGGCGA GGGCCCGGGC TGGGAGGGCA GCACCACGCT GGATGCAGGC 
TCCAGCCCCT TCCTTCCTGT GAGCCCAGAG GTCATGAAGC GGAGGCGAGG GGGGCTCATC 
GAGCAGCGGG ACATCATCAA GGCCCACGAG GCACACAAGA TGCAGAGCAC CCCGCAGGCC 
CGGCGCAAGG AGTGGGAGAT GGCTCGCTTC GGGGAGGCGG TGGTCGTTGG CAGGCCAGGC 
TCAGGCGATG GAGACTCGGA CCAGAGCAGG AACCGACAAG GAACCCCCGT CCCGGCCTCG 
GGGCCGGCGG CCGCCTACAA GCAGTCAAAA GCGCAGAGGG CGCGGACTAT GGCTTTGTAC 
AACCCCATAC CAGTCCGGCA GAACTGTTTC ACGGTCAACA GATCCCTGTT CATCTTCGGA 
GAAGATAACA TTGTCAGGAA ATACGCCAAG AAGCTCATCG ATTGGCCGCC ATTTGAGTAC 
ATGATCCTGG CCACCATCAT TGCCAACTGC ATCGTCTTGG CCCTGGAGCA GCATCTTCCT 
GAGGATGACA AGACCCCAAT GTCTCGAAGA CTGGAGAAGA CAGAACCATA TTTCATTGGG 
ATCTTCTGCT TTGAAGCTGG GATCAAAATT GTGGCTCTAG GGTTCATCTT CCATAAGGGT 
TCATACCTCC GTAATGGCTG GAATGTCATG GACTTCATCG TGGTCCTTAG TGGCATCCTG 
GCCACTGCGG GAACCCACTT CAACACCCAC GTGGACCTGC GGACCCTCCG GGCTGTGCGT 
GTCCTGAGGC CTTTAAAGCT GGTATCAGGA ATACCTAGCC TGCAGATCGT GCTGAAGTCT 
ATCATGAAGG CCATGGTGCC TCTTCTGCAG ATTGGCCTTC TGCTCTTCTT TGCCATCCTC 
ATGTTTGCTA TCATTGGCTT GGAGTTCTAC AGTGGCAAGT TGCATCGAGC ATGCTTCATG 
AACAATTCAG GTATTCTAGA GGGGTTTGAT CCTCCTCACC CGTGTGGTGT GCAAGGCTGC 
CCGGCTGGTT ATGAATGTAA GGACTGGATC GGCCCCAATG ACGGGATCAC CCAGTTTGAC 
AACATCCTTT TTGCCGTGCT GACTGTCTTC CAATGCATCA CTATGGAAGG GTGGACCACT 
GTGCTGTACA ATACCAATGA TGCCTTAGGA GCCACCTGGA ACTGGCTGTA CTTCATCCCT 
CTCATCATCA TCGGATCCTT CTTTGTTCTC AACCTCGTCC TGGGAGTGCT TTCCGGGGAA 
TTTGCCAAAG AAAGAGAGAG AGTGGAGAAC CGAAGAGCGT TCATGAAGCT CCGGCGCCAG 
CAACAGATTG AGCGAGAATT GAACGGCTAC CGTGCCTGGA TAGACAAAGC AGAGGAAGTC 
ATGCTTGCTG AAGAAAATAA AAACTCGGGA ACATCAGCCT TGGAAGTGCT TCGAAGGGCA 
ACCATCAAAA GGAGCCGGAC GGAGGCCATG ACCCGAGACT CCAGCGATGA GCACTGCGTT 
GATATCTCTT CAGTGGGCAC ACCTCTTGCC AGAGCCAGTA TCAAGAGCAC AAAAGTAGAT 
GGGGCCTCCT ATTTCCGGCA CAAGGAACGG CTTCTGCGCA TCTCTATCCG CCACATGGTC 
AAGTCCCAAG TCTTTTACTG GATCGTCCTG AGTGTTGTGG CTCTTAATAC TGCCTGTGTG 
GCCATTGTTC ATCACAACCA GCCACAGTGG CTCACTCACC TCCTCTACTA TGCAGAGTTT 
TTGTTTCTGG GACTCTTCCT GTTGGAGATG TCCCTGAAGA TGTATGGCAT GGGGCCACGC 
CTCTATTTTC ACTCATCCTT CAACTGCTTT GATTTTGGGG TCACAGTGGG CAGCATCTTT 
GAGGTTGTCT GGGCAATCTT CAGACCTGGC ACATCTTTTG GAATCAGTGT CTTACGAGCT 
CTCCGGCTCC TAAGGATATT TAAAATAACC AAGTATTGGG CATCCCTCCG GAATCTGGTT 
GTCTCCTTGA TGAGCTCCAT GAAGTCTATC ATCAGCTTAC TCTTCCTTCT CTTCCTCTTC 
ATCGTTGTCT TTGCTCTCCT GGGAATGCAG CTCTTTGGAG GCAGGTTTAA CTTTAATGAT 
GGGACTCCTT CAGCTAATTT TGACACCTTC CCTGCAGCTA TCATGACAGT GTTCCAGATC 
CTGACAGGGG AAGACTGGAA TGAGGTGATG TACAATGGCA TTCGCTCCCA GGGTGGGGTC 
AGCTCAGGCA TGTGGTCTGC CATCTACTTC ATTGTGCTCA CCTTGTTTGG AAACTACACG 
CTGCTAAATG TATTCTTGGC CATCGCTGTG GACAATCTGG CCAATGCCCA GGAACTGACC 
AAGGATGAGC AGGAGGAAGA AGAAGCCTTC AACCAGAAAC ATGCACTGCA GAAGGCCAAG 
GAGGTCAGCC CGATGTCTGC TCCCAACATG CCTTCCATTG AGAGGGAGCG GAGGCGCCGG 
CACCACATGT CTGTGTGGGA ACAACGCACC AGCCAGCTGA GGAGGCACAT GCAAATGTCT 
AGCCAAGAAG CCCTCAACAA AGAGGAGGCC CCGCCAATGA ACCCCCTCAA TCCACTCAAC 
CCACTGAGTC CTCTCAACCC CCTCAATGCT CACCCCAGCC TTTATCGGAG GCCCAGGCCC 
ATTGAGGGCC TAGCCCTAGG TCTGGGTCTT GAAAAGTGTG AAGAAGAACG CATCAGTCGT 
GGGGGCTCCC TCAAGGGGGA CATAGGGGGC CTGACTAGCG CCCTGGACAA CCAGAGGAGC 
CCCTTGTCCT TGGGCAAACG GGAGCCACCG TGGCTGCCTA GATCCTGTCA TGGAAACTGT 
GACCCAATCC AGCAGGAGGC TGGGGGAGGA GAGACTGTGG TGACTTTTGA GGACCGGGCC 
AGGCACAGGC AGAGTCAGAG GCGAAGCCGG CATCGCCGAG TCAGGACTGA GGGCAAGGAC 
TCTGCCTCTG CATCCCGAAG CAGGTCTGCC AGCCAGGAGC GGAGTCTGGA TGAAGGGGTG 
TCCGTTGAGG GGGAGAAGGA ACATGAGCCT CACAGCAGCC ACAGGAGCAA AGAGCCGACC 
ATCCACGAAG AGGAGAGAAC CCAGGACTTA AGGAGGACCA ACAGTCTGAT GGTCCCCAGG 
GGCTCTGGAC TGGTAGGGGC TCTTGATGAA GCAGAAACAC CTCTAGTCCA GCCCCAACCT 
GAGCTGGAAG TGGGGAAGGA TGCAGCTCTG ACAGAGCAGG AGGCTGAAGG CAGCAGTGAA 
CAAGCCCTTC TTGGGGACGT ACAGCTGGAT GTGGGCCGGG GCATTAGCCA GAGTGAACCG 
GATCTCTCCT GCATGACAGC CAACATGGAC AAGGCTACCA CGGAGAGCAC CAGTGTCACG 
GTCGCCATCC CTGATGTGGA CCCCTTGGTG GACTCGACAG TGGTGAACAT TAGCAACAAG 
ACGGATGGAG AAGCCAGTCC CTTGAAGGAG GCAGAGACTA AAGAGGAAGA AGAAGAGGTA 
GAGAAGAAGA AGAAGCAGAA GAAGGAGAAG CGAGAGACTG GAAAAGCCAT GGTGCCCCAT 
AGCTCTATGT TCATCTTCAG CACCACTAAC CCGATCCGCA GGGCCTGCCA CTACATTGTG 
AACCTACGCT ACTTTGAAAT GTGCATTCTC TTGGTGATTG CGGCCAGCAG CATTGCCCTG 
GCAGCCGAGG ACCCTGTACT AACTAACTCG GAGCGTAACA AAGTCCTGAG ATATTTCGAC 
TATGTTTTCA CAGGAGTGTT TACCTTTGAG ATGGTTATAA AGATGATAGA CCAAGGCTTG 
ATCCTGCAGG ATGGATCATA CTTCAGAGAC CTCTGGAACA TCCTGGACTT TGTAGTAGTG 
GTTGGTGCAT TGGTAGCATT TGCTCTGGCG AACGCTTTGG GAACCAACAA GGGGAGGGAT 
ATCAAGACTA TCAAGTCTCT GAGGGTACTT CGAGTTCTGC GGCCACTGAA AACCATCAAG 
CGCTTGCCCA AGCTGAAGGC TGTGTTTGAC TGCGTGGTGA CCTCCTTGAA GAATGTCTTC 
AACATACTCA TTGTCTACAA GCTCTTCATG TTCATCTTTG CCGTCATCGC TGTTCAGCTA 
TTCAAAGGAA AGTTCTTTTA TTGCACTGAC AGTTCCAAGG ACACAGAGAA GGAGTGCATA 
GGCAACTATG TAGACCACGA GAAAAACAAG ATGGAGGTGA AGGGCCGGGA ATGGAAGCGT 
CATGAATTCC ACTATGACAA CATCATCTGG GCCCTGCTTA CCCTCTTCAC AGTCTCTACA 
GGGGAAGGAT GGCCTCAGGT TCTGCAGCAC TCTGTAGATG TGACAGAGGA GGACAGAGGC 
CCAAGTCGCA GCAACCGCAT GGAGATGTCC ATCTTTTATG TGGTCTACTT TGTGGTCTTC 
CCTTTCTTCT TTGTCAATAT CTTCGTGGCT CTCATTATCA TCACCTTCCA GGAGCAAGGG 
GACAAGATGA TGGAGGAGTG TAGCCTGGAG AAGAATGAGC GGGCATGCAT CGACTTCGCC 
ATTAGTGCCA AACCTCTCAC CCGCTACATG CCACAGAACA GGCACACCTT CCAGTACCGT 
GTGTGGCACT TTGTAGTCTC CCCATCCTTT GAGTATACCA TCATGGCCAT GATTGCCTTG 
AACACTGTCG TGCTGATGAT GAAGTACTAC TCTGCTCCTT GCACCTATGA GCTCGCCTTA 
AAGTACCTGA ACATCGCCTT CACTATGGTG TTTTCCCTGG AGTGTGTCCT AAAGGTCATT 
GCTTTCGGCT TCCTGAACTA TTTCCGAGAT ACCTGGAACA TCTTTGACTT CATCACGGTG 
ATCGGCAGTA TCACAGAAAT TATCCTGACA GATAGCAAGC TGGTGAACAC CAGTGGCTTC 
AATATGAGCT TTCTGAAGCT CTTCCGAGCT GCACGGCTCA TAAAGCTTCT GAGACAGGGC 
TACACCATCC GCATCTTACT GTGGACCTTC GTGCAGTCCT TTAAGGCTCT TCCCTACGTC 
TGCCTTCTCA TTGCCATGCT TTTCTTCATC TACGCCATCA TTGGGATGCA GGTGTTTGGA 
AACATAAAGT TAGATGAGGA GAGTCACATC AACCGGCACA ACAACTTCCG GAGTTTCTTT 
GGATCCCTCA TGCTGCTTTT CAGGAGTGCC ACGGGGGAGG CTTGGCAGGA GATTATGCTG 
TCCTGTCTCG GTGAGAAGGG TTGTGAGCCT GACACCACTG CACCCTCAGG GCAGAATGAG 
AGTGAGCGCT GTGGCACTGA CCTGGCCTAC GTTTATTTTG TCTCCTTCAT CTTCTTCTGC 
TCCTTCCTGA TGCTCAACCT GTTCGTGGCT GTCATCATGG ACAACTTTGA GTACCTGACT 
CGAGATTCCT CCATCCTGGG GCCTCACCAC TTAGATGAGT TTGTCCGTGT CTGGGCAGAA 
TATGACAGAG CAGCATGTGG CCGCATCCAT TACACTGAGA TGTATGAAAT GCTGACTCTC 
ATGTCACCAC CGCTAGGCCT CGGCAAGAGA TGTCCCTCCA AAGTGGCCTA TAAGAGGCTG 
GTCCTGATGA ACATGCCAGT GGCTGAGGAC ATGACAGTCC ACTTCACCTC CACACTTATG 
GCTCTGATCC GGACAGCTCT GGACATTAAA ATCGCCAAAG GTGGTGCAGA CAGACAGCAG 
CTAGACTCGG AGCTGCAAAA AGAGACCCTG GCCATTTGGC CTCACCTGTC CCAAAAGATG 
TTGGATCTGC TTGTGCCCAT GCCCAAAGCC TCTGACCTGA CTGTGGGCAA GATCTATGCA 
GCAATGATGA TCATGGACTA CTATAAACAG AGTAAGGTAA AGAAACAGAG ACAGCAGCTG 
GAAGAACAGA AAAATGCACC CATGTTCCAG CGCATGGAGC CCTCATCACT GCCTCAGGAG 
ATCATTGCTA ATGCCAAAGC CCTGCCTTAT CTCCAGCAGG ACCCTGTTTC TGGCCTGAGT 
GGTCGGAGTG GATACCCTTC AATGAGTCCA CTCTCCCCTC AAGAAATATT CCAGTTGGCT 
TGTATGGACC CAGCGGATGA TGGACAATTC CAGGAACAAC AATCTCTGGA GCCAGAGGTT 
AGTGAATTAA AAAGTGTGCA GTCCTCTAAC CATGGCATCT ACCTTCCTCC GGACACCCAG 
GAGCATGCGG GATCTGGGAG GGCATCCTCT ATGCCACGTC TGACTATGGA TCCCCAGGTG 
GTAACAGACC CTAGCTCTAT GCGACGTTCA TTTTCTACAA TTCGGGATAA GCGTTCAAAT 
TCCTCCTGGT TGGAGGAGTT CTCCATGGAA CGCAGCAGTG AAAACACTTA CAAGTCGCGG 
CGCAGAAGTT ACCATTCCTC CCTGAGGCTG TCAGCACACC GCCTGAATTC TGACTCTGGT 
CACAAGTCTG ACACTCACCG ATCAGGAGGC AGGGAGAGAG GCCGATCCAA AGAACGAAAG 
CACCTCCTCT CTCCAGATGT CTCCCGCTGC AATTCTGAAG AGCGAGGGAC CCAGGCCGAC 
TGGGAGTCCC CAGAACGTCG CCAGTCCAGG TCACCCAGTG AAGGAAGGTC ACAGACCCCC 
AATAGACAGG GTACCGGTTC CCTAAGTGAG AGCTCCATCC CCTCTATTTC CGACACCAGC 
ACCCCAAGAC GAAGCCGTCG CCAGCTCCCA CCTGTGCCAC CAAAGCCTCG TCCCCTCCTC 
TCCTACAGCT CCCTGATGAG ACACACTGGC GGCATCTCTC CACCTCCTGA TGGAAGTGAG 
GGTGGATCCC CACTGGCCTC TCAAGCTCTG GAGAGCAACA GCGCTTGCCT GACCGAGTCT 
TCCAACTCCT TGCACCCCCA GCAGGGCCAG CATCCTTCCC CACAGCACTA CATCTCCGAG 
CCCTATCTGG CCCTCCATGA AGACTCCCAC GCCTCAGACT GTGGCGAGGA GGAGACACTC 
ACCTTTGAGG CAGCCGTAGC CACTAGCTTG GGCCGGTCCA ATACCATTGG CTCTGCGCCA 
CCCCTACGGC ACAGCTGGCA GATGCCTAAT GGGCACTATC GGCGGCGGAG GCGTGGGGGG 
CCTGGGCCTG GCATGATGTG TGGAGCTGTC AGTGACCTCC TGAGTGACAC GGAAGAAGAC 
GATAAGTGCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cacna1e ( XM_017312745.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017168234.1 Mus musculus calcium channel, voltage-dependent, R type, alpha 1E subunit (Cacna1e), transcript variant X13, mRNA.

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CloneID	OMu27876
Clone ID Related Accession (Same CDS sequence)	XM_017312745.1
Accession Version	XM_017312745.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7260bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	voltage-dependent R-type calcium channel subunit alpha-1E isoform X10
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGCCCG AGATGCGTGG ACTGAGCCCA GGCCGAAGGC TGCCCGAGCC CACCCTGTGC 
CCGGCTACAG GAGGGAGCCC TGAGCCCGGG GCTGCTGGCA GGCGCCGCCA CGTCCTGGCT 
CTGCCTGATG AGGAGGCATG GCGGCCGCAG CAGTGCGCCC TGCTCGAGGC CAATGCCTCC 
GACGAGGTGG GCATGTTGCC TGCTTCCCCG CGCTCCGCGG TGGCCGGCTT CGACAACTTC 
TTCCCGGTGC AGGAAGGCGA GGGCCCGGGC TGGGAGGGCA GCACCACGCT GGATGCAGGC 
TCCAGCCCCT TCCTTCCTGT GAGCCCAGAG GTCATGAAGC GGAGGCGAGG GGGGCTCATC 
GAGCAGCGGG ACATCATCAA GGCCCACGAG GCACACAAGA TGCAGAGCAC CCCGCAGGCC 
CGGCGCAAGG AGTGGGAGAT GGCTCGCTTC GGGGAGGCGG TGGTCGTTGG CAGGCCAGGC 
TCAGGCGATG GAGACTCGGA CCAGAGCAGG AACCGACAAG GAACCCCCGT CCCGGCCTCG 
GGGCCGGCGG CCGCCTACAA GCAGTCAAAA GCGCAGAGGG CGCGGACTAT GGCTTTGTAC 
AACCCCATAC CAGTCCGGCA GAACTGTTTC ACGGTCAACA GATCCCTGTT CATCTTCGGA 
GAAGATAACA TTGTCAGGAA ATACGCCAAG AAGCTCATCG ATTGGCCGCC ATTTGAGTAC 
ATGATCCTGG CCACCATCAT TGCCAACTGC ATCGTCTTGG CCCTGGAGCA GCATCTTCCT 
GAGGATGACA AGACCCCAAT GTCTCGAAGA CTGGAGAAGA CAGAACCATA TTTCATTGGG 
ATCTTCTGCT TTGAAGCTGG GATCAAAATT GTGGCTCTAG GGTTCATCTT CCATAAGGGT 
TCATACCTCC GTAATGGCTG GAATGTCATG GACTTCATCG TGGTCCTTAG TGGCATCCTG 
GCCACTGCGG GAACCCACTT CAACACCCAC GTGGACCTGC GGACCCTCCG GGCTGTGCGT 
GTCCTGAGGC CTTTAAAGCT GGTATCAGGA ATACCTAGCC TGCAGATCGT GCTGAAGTCT 
ATCATGAAGG CCATGGTGCC TCTTCTGCAG ATTGGCCTTC TGCTCTTCTT TGCCATCCTC 
ATGTTTGCTA TCATTGGCTT GGAGTTCTAC AGTGGCAAGT TGCATCGAGC ATGCTTCATG 
AACAATTCAG GTATTCTAGA GGGGTTTGAT CCTCCTCACC CGTGTGGTGT GCAAGGCTGC 
CCGGCTGGTT ATGAATGTAA GGACTGGATC GGCCCCAATG ACGGGATCAC CCAGTTTGAC 
AACATCCTTT TTGCCGTGCT GACTGTCTTC CAATGCATCA CTATGGAAGG GTGGACCACT 
GTGCTGTACA ATACCAATGA TGCCTTAGGA GCCACCTGGA ACTGGCTGTA CTTCATCCCT 
CTCATCATCA TCGGATCCTT CTTTGTTCTC AACCTCGTCC TGGGAGTGCT TTCCGGGGAA 
TTTGCCAAAG AAAGAGAGAG AGTGGAGAAC CGAAGAGCGT TCATGAAGCT CCGGCGCCAG 
CAACAGATTG AGCGAGAATT GAACGGCTAC CGTGCCTGGA TAGACAAAGC AGAGGAAGTC 
ATGCTTGCTG AAGAAAATAA AAACTCGGGA ACATCAGCCT TGGAAGTGCT TCGAAGGGCA 
ACCATCAAAA GGAGCCGGAC GGAGGCCATG ACCCGAGACT CCAGCGATGA GCACTGCGTT 
GATATCTCTT CAGTGGGCAC ACCTCTTGCC AGAGCCAGTA TCAAGAGCAC AAAAGTAGAT 
GGGGCCTCCT ATTTCCGGCA CAAGGAACGG CTTCTGCGCA TCTCTATCCG CCACATGGTC 
AAGTCCCAAG TCTTTTACTG GATCGTCCTG AGTGTTGTGG CTCTTAATAC TGCCTGTGTG 
GCCATTGTTC ATCACAACCA GCCACAGTGG CTCACTCACC TCCTCTACTA TGCAGAGTTT 
TTGTTTCTGG GACTCTTCCT GTTGGAGATG TCCCTGAAGA TGTATGGCAT GGGGCCACGC 
CTCTATTTTC ACTCATCCTT CAACTGCTTT GATTTTGGGG TCACAGTGGG CAGCATCTTT 
GAGGTTGTCT GGGCAATCTT CAGACCTGGC ACATCTTTTG GAATCAGTGT CTTACGAGCT 
CTCCGGCTCC TAAGGATATT TAAAATAACC AAGTATTGGG CATCCCTCCG GAATCTGGTT 
GTCTCCTTGA TGAGCTCCAT GAAGTCTATC ATCAGCTTAC TCTTCCTTCT CTTCCTCTTC 
ATCGTTGTCT TTGCTCTCCT GGGAATGCAG CTCTTTGGAG GCAGGTTTAA CTTTAATGAT 
GGGACTCCTT CAGCTAATTT TGACACCTTC CCTGCAGCTA TCATGACAGT GTTCCAGATC 
CTGACAGGGG AAGACTGGAA TGAGGTGATG TACAATGGCA TTCGCTCCCA GGGTGGGGTC 
AGCTCAGGCA TGTGGTCTGC CATCTACTTC ATTGTGCTCA CCTTGTTTGG AAACTACACG 
CTGCTAAATG TATTCTTGGC CATCGCTGTG GACAATCTGG CCAATGCCCA GGAACTGACC 
AAGGATGAGC AGGAGGAAGA AGAAGCCTTC AACCAGAAAC ATGCACTGCA GAAGGCCAAG 
GAGGTCAGCC CGATGTCTGC TCCCAACATG CCTTCCATTG AAAGAGACAG AAGGAGAAGA 
CACCACATGT CGATGTGGGA GCCACGCAGC AGCCACCTGA GGGAGCGGAG GCGCCGGCAC 
CACATGTCTG TGTGGGAACA ACGCACCAGC CAGCTGAGGA GGCACATGCA AATGTCTAGC 
CAAGAAGCCC TCAACAAAGA GGAGGCCCCG CCAATGAACC CCCTCAATCC ACTCAACCCA 
CTGAGTCCTC TCAACCCCCT CAATGCTCAC CCCAGCCTTT ATCGGAGGCC CAGGCCCATT 
GAGGGCCTAG CCCTAGGTCT GGGTCTTGAA AAGTGTGAAG AAGAACGCAT CAGTCGTGGG 
GGCTCCCTCA AGGGGGACAT AGGGGGCCTG ACTAGCGCCC TGGACAACCA GAGGAGCCCC 
TTGTCCTTGG GCAAACGGGA GCCACCGTGG CTGCCTAGAT CCTGTCATGG AAACTGTGAC 
CCAATCCAGC AGGAGGCTGG GGGAGGAGAG ACTGTGGTGA CTTTTGAGGA CCGGGCCAGG 
CACAGGCAGA GTCAGAGGCG AAGCCGGCAT CGCCGAGTCA GGACTGAGGG CAAGGACTCT 
GCCTCTGCAT CCCGAAGCAG GTCTGCCAGC CAGGAGCGGA GTCTGGATGA AGGGGTGTCC 
GTTGAGGGGG AGAAGGAACA TGAGCCTCAC AGCAGCCACA GGAGCAAAGA GCCGACCATC 
CACGAAGAGG AGAGAACCCA GGACTTAAGG AGGACCAACA GTCTGATGGT CCCCAGGGGC 
TCTGGACTGG TAGGGGCTCT TGATGAAGCA GAAACACCTC TAGTCCAGCC CCAACCTGAG 
CTGGAAGTGG GGAAGGATGC AGCTCTGACA GAGCAGGAGG CTGAAGGCAG CAGTGAACAA 
GCCCTTCTTG GGGACGTACA GCTGGATGTG GGCCGGGGCA TTAGCCAGAG TGAACCGGAT 
CTCTCCTGCA TGACAGCCAA CATGGACAAG GCTACCACGG AGAGCACCAG TGTCACGGTC 
GCCATCCCTG ATGTGGACCC CTTGGTGGAC TCGACAGTGG TGAACATTAG CAACAAGACG 
GATGGAGAAG CCAGTCCCTT GAAGGAGGCA GAGACTAAAG AGGAAGAAGA AGAGGTAGAG 
AAGAAGAAGA AGCAGAAGAA GGAGAAGCGA GAGACTGGAA AAGCCATGGT GCCCCATAGC 
TCTATGTTCA TCTTCAGCAC CACTAACCCG ATCCGCAGGG CCTGCCACTA CATTGTGAAC 
CTACGCTACT TTGAAATGTG CATTCTCTTG GTGATTGCGG CCAGCAGCAT TGCCCTGGCA 
GCCGAGGACC CTGTACTAAC TAACTCGGAG CGTAACAAAG TCCTGAGATA TTTCGACTAT 
GTTTTCACAG GAGTGTTTAC CTTTGAGATG GTTATAAAGA TGATAGACCA AGGCTTGATC 
CTGCAGGATG GATCATACTT CAGAGACCTC TGGAACATCC TGGACTTTGT AGTAGTGGTT 
GGTGCATTGG TAGCATTTGC TCTGGCGAAC GCTTTGGGAA CCAACAAGGG GAGGGATATC 
AAGACTATCA AGTCTCTGAG GGTACTTCGA GTTCTGCGGC CACTGAAAAC CATCAAGCGC 
TTGCCCAAGC TGAAGGCTGT GTTTGACTGC GTGGTGACCT CCTTGAAGAA TGTCTTCAAC 
ATACTCATTG TCTACAAGCT CTTCATGTTC ATCTTTGCCG TCATCGCTGT TCAGCTATTC 
AAAGGAAAGT TCTTTTATTG CACTGACAGT TCCAAGGACA CAGAGAAGGA GTGCATAGGC 
AACTATGTAG ACCACGAGAA AAACAAGATG GAGGTGAAGG GCCGGGAATG GAAGCGTCAT 
GAATTCCACT ATGACAACAT CATCTGGGCC CTGCTTACCC TCTTCACAGT CTCTACAGGG 
GAAGGATGGC CTCAGGTTCT GCAGCACTCT GTAGATGTGA CAGAGGAGGA CAGAGGCCCA 
AGTCGCAGCA ACCGCATGGA GATGTCCATC TTTTATGTGG TCTACTTTGT GGTCTTCCCT 
TTCTTCTTTG TCAATATCTT CGTGGCTCTC ATTATCATCA CCTTCCAGGA GCAAGGGGAC 
AAGATGATGG AGGAGTGTAG CCTGGAGAAG AATGAGCGGG CATGCATCGA CTTCGCCATT 
AGTGCCAAAC CTCTCACCCG CTACATGCCA CAGAACAGGC ACACCTTCCA GTACCGTGTG 
TGGCACTTTG TAGTCTCCCC ATCCTTTGAG TATACCATCA TGGCCATGAT TGCCTTGAAC 
ACTGTCGTGC TGATGATGAA GTACTACTCT GCTCCTTGCA CCTATGAGCT CGCCTTAAAG 
TACCTGAACA TCGCCTTCAC TATGGTGTTT TCCCTGGAGT GTGTCCTAAA GGTCATTGCT 
TTCGGCTTCC TGAACTATTT CCGAGATACC TGGAACATCT TTGACTTCAT CACGGTGATC 
GGCAGTATCA CAGAAATTAT CCTGACAGAT AGCAAGCTGG TGAACACCAG TGGCTTCAAT 
ATGAGCTTTC TGAAGCTCTT CCGAGCTGCA CGGCTCATAA AGCTTCTGAG ACAGGGCTAC 
ACCATCCGCA TCTTACTGTG GACCTTCGTG CAGTCCTTTA AGGCTCTTCC CTACGTCTGC 
CTTCTCATTG CCATGCTTTT CTTCATCTAC GCCATCATTG GGATGCAGGT GTTTGGAAAC 
ATAAAGTTAG ATGAGGAGAG TCACATCAAC CGGCACAACA ACTTCCGGAG TTTCTTTGGA 
TCCCTCATGC TGCTTTTCAG GAGTGCCACG GGGGAGGCTT GGCAGGAGAT TATGCTGTCC 
TGTCTCGGTG AGAAGGGTTG TGAGCCTGAC ACCACTGCAC CCTCAGGGCA GAATGAGAGT 
GAGCGCTGTG GCACTGACCT GGCCTACGTT TATTTTGTCT CCTTCATCTT CTTCTGCTCC 
TTCCTGATGC TCAACCTGTT CGTGGCTGTC ATCATGGACA ACTTTGAGTA CCTGACTCGA 
GATTCCTCCA TCCTGGGGCC TCACCACTTA GATGAGTTTG TCCGTGTCTG GGCAGAATAT 
GACAGAGCAG CATGTGGCCG CATCCATTAC ACTGAGATGT ATGAAATGCT GACTCTCATG 
TCACCACCGC TAGGCCTCGG CAAGAGATGT CCCTCCAAAG TGGCCTATAA GAGGCTGGTC 
CTGATGAACA TGCCAGTGGC TGAGGACATG ACAGTCCACT TCACCTCCAC ACTTATGGCT 
CTGATCCGGA CAGCTCTGGA CATTAAAATC GCCAAAGGTG GTGCAGACAG ACAGCAGCTA 
GACTCGGAGC TGCAAAAAGA GACCCTGGCC ATTTGGCCTC ACCTGTCCCA AAAGATGTTG 
GATCTGCTTG TGCCCATGCC CAAAGCCTCT GACCTGACTG TGGGCAAGAT CTATGCAGCA 
ATGATGATCA TGGACTACTA TAAACAGAGT AAGGTAAAGA AACAGAGACA GCAGCTGGAA 
GAACAGAAAA ATGCACCCAT GTTCCAGCGC ATGGAGCCCT CATCACTGCC TCAGGAGATC 
ATTGCTAATG CCAAAGCCCT GCCTTATCTC CAGCAGGACC CTGTTTCTGG CCTGAGTGGT 
CGGAGTGGAT ACCCTTCAAT GAGTCCACTC TCCCCTCAAG AAATATTCCA GTTGGCTTGT 
ATGGACCCAG CGGATGATGG ACAATTCCAG GAACAACAAT CTCTGGTGGT AACAGACCCT 
AGCTCTATGC GACGTTCATT TTCTACAATT CGGGATAAGC GTTCAAATTC CTCCTGGTTG 
GAGGAGTTCT CCATGGAACG CAGCAGTGAA AACACTTACA AGTCGCGGCG CAGAAGTTAC 
CATTCCTCCC TGAGGCTGTC AGCACACCGC CTGAATTCTG ACTCTGGTCA CAAGTCTGAC 
ACTCACCGAT CAGGAGGCAG GGAGAGAGGC CGATCCAAAG AACGAAAGCA CCTCCTCTCT 
CCAGATGTCT CCCGCTGCAA TTCTGAAGAG CGAGGGACCC AGGCCGACTG GGAGTCCCCA 
GAACGTCGCC AGTCCAGGTC ACCCAGTGAA GGAAGGTCAC AGACCCCCAA TAGACAGGGT 
ACCGGTTCCC TAAGTGAGAG CTCCATCCCC TCTATTTCCG ACACCAGCAC CCCAAGACGA 
AGCCGTCGCC AGCTCCCACC TGTGCCACCA AAGCCTCGTC CCCTCCTCTC CTACAGCTCC 
CTGATGAGAC ACACTGGCGG CATCTCTCCA CCTCCTGATG GAAGTGAGGG TGGATCCCCA 
CTGGCCTCTC AAGCTCTGGA GAGCAACAGC GCTTGCCTGA CCGAGTCTTC CAACTCCTTG 
CACCCCCAGC AGGGCCAGCA TCCTTCCCCA CAGCACTACA TCTCCGAGCC CTATCTGGCC 
CTCCATGAAG ACTCCCACGC CTCAGACTGT GGCGAGGAGG AGACACTCAC CTTTGAGGCA 
GCCGTAGCCA CTAGCTTGGG CCGGTCCAAT ACCATTGGCT CTGCGCCACC CCTACGGCAC 
AGCTGGCAGA TGCCTAATGG GCACTATCGG CGGCGGAGGC GTGGGGGGCC TGGGCCTGGC 
ATGATGTGTG GAGCTGTCAG TGACCTCCTG AGTGACACGG AAGAAGACGA TAAGTGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017168234.1
CDS	594..7853
Translation	MVPEMRGLSPGRRLPEPTLCPATGGSPEPGAAGRRRHVLALPDEEAWRPQQCALLEANASDEVGMLPASPRSAVAGFDNFFPVQEGEGPGWEGSTTLDAGSSPFLPVSPEVMKRRRGGLIEQRDIIKAHEAHKMQSTPQARRKEWEMARFGEAVVVGRPGSGDGDSDQSRNRQGTPVPASGPAAAYKQSKAQRARTMALYNPIPVRQNCFTVNRSLFIFGEDNIVRKYAKKLIDWPPFEYMILATIIANCIVLALEQHLPEDDKTPMSRRLEKTEPYFIGIFCFEAGIKIVALGFIFHKGSYLRNGWNVMDFIVVLSGILATAGTHFNTHVDLRTLRAVRVLRPLKLVSGIPSLQIVLKSIMKAMVPLLQIGLLLFFAILMFAIIGLEFYSGKLHRACFMNNSGILEGFDPPHPCGVQGCPAGYECKDWIGPNDGITQFDNILFAVLTVFQCITMEGWTTVLYNTNDALGATWNWLYFIPLIIIGSFFVLNLVLGVLSGEFAKERERVENRRAFMKLRRQQQIERELNGYRAWIDKAEEVMLAEENKNSGTSALEVLRRATIKRSRTEAMTRDSSDEHCVDISSVGTPLARASIKSTKVDGASYFRHKERLLRISIRHMVKSQVFYWIVLSVVALNTACVAIVHHNQPQWLTHLLYYAEFLFLGLFLLEMSLKMYGMGPRLYFHSSFNCFDFGVTVGSIFEVVWAIFRPGTSFGISVLRALRLLRIFKITKYWASLRNLVVSLMSSMKSIISLLFLLFLFIVVFALLGMQLFGGRFNFNDGTPSANFDTFPAAIMTVFQILTGEDWNEVMYNGIRSQGGVSSGMWSAIYFIVLTLFGNYTLLNVFLAIAVDNLANAQELTKDEQEEEEAFNQKHALQKAKEVSPMSAPNMPSIERDRRRRHHMSMWEPRSSHLRERRRRHHMSVWEQRTSQLRRHMQMSSQEALNKEEAPPMNPLNPLNPLSPLNPLNAHPSLYRRPRPIEGLALGLGLEKCEEERISRGGSLKGDIGGLTSALDNQRSPLSLGKREPPWLPRSCHGNCDPIQQEAGGGETVVTFEDRARHRQSQRRSRHRRVRTEGKDSASASRSRSASQERSLDEGVSVEGEKEHEPHSSHRSKEPTIHEEERTQDLRRTNSLMVPRGSGLVGALDEAETPLVQPQPELEVGKDAALTEQEAEGSSEQALLGDVQLDVGRGISQSEPDLSCMTANMDKATTESTSVTVAIPDVDPLVDSTVVNISNKTDGEASPLKEAETKEEEEEVEKKKKQKKEKRETGKAMVPHSSMFIFSTTNPIRRACHYIVNLRYFEMCILLVIAASSIALAAEDPVLTNSERNKVLRYFDYVFTGVFTFEMVIKMIDQGLILQDGSYFRDLWNILDFVVVVGALVAFALANALGTNKGRDIKTIKSLRVLRVLRPLKTIKRLPKLKAVFDCVVTSLKNVFNILIVYKLFMFIFAVIAVQLFKGKFFYCTDSSKDTEKECIGNYVDHEKNKMEVKGREWKRHEFHYDNIIWALLTLFTVSTGEGWPQVLQHSVDVTEEDRGPSRSNRMEMSIFYVVYFVVFPFFFVNIFVALIIITFQEQGDKMMEECSLEKNERACIDFAISAKPLTRYMPQNRHTFQYRVWHFVVSPSFEYTIMAMIALNTVVLMMKYYSAPCTYELALKYLNIAFTMVFSLECVLKVIAFGFLNYFRDTWNIFDFITVIGSITEIILTDSKLVNTSGFNMSFLKLFRAARLIKLLRQGYTIRILLWTFVQSFKALPYVCLLIAMLFFIYAIIGMQVFGNIKLDEESHINRHNNFRSFFGSLMLLFRSATGEAWQEIMLSCLGEKGCEPDTTAPSGQNESERCGTDLAYVYFVSFIFFCSFLMLNLFVAVIMDNFEYLTRDSSILGPHHLDEFVRVWAEYDRAACGRIHYTEMYEMLTLMSPPLGLGKRCPSKVAYKRLVLMNMPVAEDMTVHFTSTLMALIRTALDIKIAKGGADRQQLDSELQKETLAIWPHLSQKMLDLLVPMPKASDLTVGKIYAAMMIMDYYKQSKVKKQRQQLEEQKNAPMFQRMEPSSLPQEIIANAKALPYLQQDPVSGLSGRSGYPSMSPLSPQEIFQLACMDPADDGQFQEQQSLVVTDPSSMRRSFSTIRDKRSNSSWLEEFSMERSSENTYKSRRRSYHSSLRLSAHRLNSDSGHKSDTHRSGGRERGRSKERKHLLSPDVSRCNSEERGTQADWESPERRQSRSPSEGRSQTPNRQGTGSLSESSIPSISDTSTPRRSRRQLPPVPPKPRPLLSYSSLMRHTGGISPPPDGSEGGSPLASQALESNSACLTESSNSLHPQQGQHPSPQHYISEPYLALHEDSHASDCGEEETLTFEAAVATSLGRSNTIGSAPPLRHSWQMPNGHYRRRRRGGPGPGMMCGAVSDLLSDTEEDDKC

Target ORF information:

RefSeq Version	XM_017312745.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus calcium channel, voltage-dependent, R type, alpha 1E subunit (Cacna1e), transcript variant X13, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017312745.1

ORF Insert Sequence:

ATGGTGCCCG AGATGCGTGG ACTGAGCCCA GGCCGAAGGC TGCCCGAGCC CACCCTGTGC 
CCGGCTACAG GAGGGAGCCC TGAGCCCGGG GCTGCTGGCA GGCGCCGCCA CGTCCTGGCT 
CTGCCTGATG AGGAGGCATG GCGGCCGCAG CAGTGCGCCC TGCTCGAGGC CAATGCCTCC 
GACGAGGTGG GCATGTTGCC TGCTTCCCCG CGCTCCGCGG TGGCCGGCTT CGACAACTTC 
TTCCCGGTGC AGGAAGGCGA GGGCCCGGGC TGGGAGGGCA GCACCACGCT GGATGCAGGC 
TCCAGCCCCT TCCTTCCTGT GAGCCCAGAG GTCATGAAGC GGAGGCGAGG GGGGCTCATC 
GAGCAGCGGG ACATCATCAA GGCCCACGAG GCACACAAGA TGCAGAGCAC CCCGCAGGCC 
CGGCGCAAGG AGTGGGAGAT GGCTCGCTTC GGGGAGGCGG TGGTCGTTGG CAGGCCAGGC 
TCAGGCGATG GAGACTCGGA CCAGAGCAGG AACCGACAAG GAACCCCCGT CCCGGCCTCG 
GGGCCGGCGG CCGCCTACAA GCAGTCAAAA GCGCAGAGGG CGCGGACTAT GGCTTTGTAC 
AACCCCATAC CAGTCCGGCA GAACTGTTTC ACGGTCAACA GATCCCTGTT CATCTTCGGA 
GAAGATAACA TTGTCAGGAA ATACGCCAAG AAGCTCATCG ATTGGCCGCC ATTTGAGTAC 
ATGATCCTGG CCACCATCAT TGCCAACTGC ATCGTCTTGG CCCTGGAGCA GCATCTTCCT 
GAGGATGACA AGACCCCAAT GTCTCGAAGA CTGGAGAAGA CAGAACCATA TTTCATTGGG 
ATCTTCTGCT TTGAAGCTGG GATCAAAATT GTGGCTCTAG GGTTCATCTT CCATAAGGGT 
TCATACCTCC GTAATGGCTG GAATGTCATG GACTTCATCG TGGTCCTTAG TGGCATCCTG 
GCCACTGCGG GAACCCACTT CAACACCCAC GTGGACCTGC GGACCCTCCG GGCTGTGCGT 
GTCCTGAGGC CTTTAAAGCT GGTATCAGGA ATACCTAGCC TGCAGATCGT GCTGAAGTCT 
ATCATGAAGG CCATGGTGCC TCTTCTGCAG ATTGGCCTTC TGCTCTTCTT TGCCATCCTC 
ATGTTTGCTA TCATTGGCTT GGAGTTCTAC AGTGGCAAGT TGCATCGAGC ATGCTTCATG 
AACAATTCAG GTATTCTAGA GGGGTTTGAT CCTCCTCACC CGTGTGGTGT GCAAGGCTGC 
CCGGCTGGTT ATGAATGTAA GGACTGGATC GGCCCCAATG ACGGGATCAC CCAGTTTGAC 
AACATCCTTT TTGCCGTGCT GACTGTCTTC CAATGCATCA CTATGGAAGG GTGGACCACT 
GTGCTGTACA ATACCAATGA TGCCTTAGGA GCCACCTGGA ACTGGCTGTA CTTCATCCCT 
CTCATCATCA TCGGATCCTT CTTTGTTCTC AACCTCGTCC TGGGAGTGCT TTCCGGGGAA 
TTTGCCAAAG AAAGAGAGAG AGTGGAGAAC CGAAGAGCGT TCATGAAGCT CCGGCGCCAG 
CAACAGATTG AGCGAGAATT GAACGGCTAC CGTGCCTGGA TAGACAAAGC AGAGGAAGTC 
ATGCTTGCTG AAGAAAATAA AAACTCGGGA ACATCAGCCT TGGAAGTGCT TCGAAGGGCA 
ACCATCAAAA GGAGCCGGAC GGAGGCCATG ACCCGAGACT CCAGCGATGA GCACTGCGTT 
GATATCTCTT CAGTGGGCAC ACCTCTTGCC AGAGCCAGTA TCAAGAGCAC AAAAGTAGAT 
GGGGCCTCCT ATTTCCGGCA CAAGGAACGG CTTCTGCGCA TCTCTATCCG CCACATGGTC 
AAGTCCCAAG TCTTTTACTG GATCGTCCTG AGTGTTGTGG CTCTTAATAC TGCCTGTGTG 
GCCATTGTTC ATCACAACCA GCCACAGTGG CTCACTCACC TCCTCTACTA TGCAGAGTTT 
TTGTTTCTGG GACTCTTCCT GTTGGAGATG TCCCTGAAGA TGTATGGCAT GGGGCCACGC 
CTCTATTTTC ACTCATCCTT CAACTGCTTT GATTTTGGGG TCACAGTGGG CAGCATCTTT 
GAGGTTGTCT GGGCAATCTT CAGACCTGGC ACATCTTTTG GAATCAGTGT CTTACGAGCT 
CTCCGGCTCC TAAGGATATT TAAAATAACC AAGTATTGGG CATCCCTCCG GAATCTGGTT 
GTCTCCTTGA TGAGCTCCAT GAAGTCTATC ATCAGCTTAC TCTTCCTTCT CTTCCTCTTC 
ATCGTTGTCT TTGCTCTCCT GGGAATGCAG CTCTTTGGAG GCAGGTTTAA CTTTAATGAT 
GGGACTCCTT CAGCTAATTT TGACACCTTC CCTGCAGCTA TCATGACAGT GTTCCAGATC 
CTGACAGGGG AAGACTGGAA TGAGGTGATG TACAATGGCA TTCGCTCCCA GGGTGGGGTC 
AGCTCAGGCA TGTGGTCTGC CATCTACTTC ATTGTGCTCA CCTTGTTTGG AAACTACACG 
CTGCTAAATG TATTCTTGGC CATCGCTGTG GACAATCTGG CCAATGCCCA GGAACTGACC 
AAGGATGAGC AGGAGGAAGA AGAAGCCTTC AACCAGAAAC ATGCACTGCA GAAGGCCAAG 
GAGGTCAGCC CGATGTCTGC TCCCAACATG CCTTCCATTG AAAGAGACAG AAGGAGAAGA 
CACCACATGT CGATGTGGGA GCCACGCAGC AGCCACCTGA GGGAGCGGAG GCGCCGGCAC 
CACATGTCTG TGTGGGAACA ACGCACCAGC CAGCTGAGGA GGCACATGCA AATGTCTAGC 
CAAGAAGCCC TCAACAAAGA GGAGGCCCCG CCAATGAACC CCCTCAATCC ACTCAACCCA 
CTGAGTCCTC TCAACCCCCT CAATGCTCAC CCCAGCCTTT ATCGGAGGCC CAGGCCCATT 
GAGGGCCTAG CCCTAGGTCT GGGTCTTGAA AAGTGTGAAG AAGAACGCAT CAGTCGTGGG 
GGCTCCCTCA AGGGGGACAT AGGGGGCCTG ACTAGCGCCC TGGACAACCA GAGGAGCCCC 
TTGTCCTTGG GCAAACGGGA GCCACCGTGG CTGCCTAGAT CCTGTCATGG AAACTGTGAC 
CCAATCCAGC AGGAGGCTGG GGGAGGAGAG ACTGTGGTGA CTTTTGAGGA CCGGGCCAGG 
CACAGGCAGA GTCAGAGGCG AAGCCGGCAT CGCCGAGTCA GGACTGAGGG CAAGGACTCT 
GCCTCTGCAT CCCGAAGCAG GTCTGCCAGC CAGGAGCGGA GTCTGGATGA AGGGGTGTCC 
GTTGAGGGGG AGAAGGAACA TGAGCCTCAC AGCAGCCACA GGAGCAAAGA GCCGACCATC 
CACGAAGAGG AGAGAACCCA GGACTTAAGG AGGACCAACA GTCTGATGGT CCCCAGGGGC 
TCTGGACTGG TAGGGGCTCT TGATGAAGCA GAAACACCTC TAGTCCAGCC CCAACCTGAG 
CTGGAAGTGG GGAAGGATGC AGCTCTGACA GAGCAGGAGG CTGAAGGCAG CAGTGAACAA 
GCCCTTCTTG GGGACGTACA GCTGGATGTG GGCCGGGGCA TTAGCCAGAG TGAACCGGAT 
CTCTCCTGCA TGACAGCCAA CATGGACAAG GCTACCACGG AGAGCACCAG TGTCACGGTC 
GCCATCCCTG ATGTGGACCC CTTGGTGGAC TCGACAGTGG TGAACATTAG CAACAAGACG 
GATGGAGAAG CCAGTCCCTT GAAGGAGGCA GAGACTAAAG AGGAAGAAGA AGAGGTAGAG 
AAGAAGAAGA AGCAGAAGAA GGAGAAGCGA GAGACTGGAA AAGCCATGGT GCCCCATAGC 
TCTATGTTCA TCTTCAGCAC CACTAACCCG ATCCGCAGGG CCTGCCACTA CATTGTGAAC 
CTACGCTACT TTGAAATGTG CATTCTCTTG GTGATTGCGG CCAGCAGCAT TGCCCTGGCA 
GCCGAGGACC CTGTACTAAC TAACTCGGAG CGTAACAAAG TCCTGAGATA TTTCGACTAT 
GTTTTCACAG GAGTGTTTAC CTTTGAGATG GTTATAAAGA TGATAGACCA AGGCTTGATC 
CTGCAGGATG GATCATACTT CAGAGACCTC TGGAACATCC TGGACTTTGT AGTAGTGGTT 
GGTGCATTGG TAGCATTTGC TCTGGCGAAC GCTTTGGGAA CCAACAAGGG GAGGGATATC 
AAGACTATCA AGTCTCTGAG GGTACTTCGA GTTCTGCGGC CACTGAAAAC CATCAAGCGC 
TTGCCCAAGC TGAAGGCTGT GTTTGACTGC GTGGTGACCT CCTTGAAGAA TGTCTTCAAC 
ATACTCATTG TCTACAAGCT CTTCATGTTC ATCTTTGCCG TCATCGCTGT TCAGCTATTC 
AAAGGAAAGT TCTTTTATTG CACTGACAGT TCCAAGGACA CAGAGAAGGA GTGCATAGGC 
AACTATGTAG ACCACGAGAA AAACAAGATG GAGGTGAAGG GCCGGGAATG GAAGCGTCAT 
GAATTCCACT ATGACAACAT CATCTGGGCC CTGCTTACCC TCTTCACAGT CTCTACAGGG 
GAAGGATGGC CTCAGGTTCT GCAGCACTCT GTAGATGTGA CAGAGGAGGA CAGAGGCCCA 
AGTCGCAGCA ACCGCATGGA GATGTCCATC TTTTATGTGG TCTACTTTGT GGTCTTCCCT 
TTCTTCTTTG TCAATATCTT CGTGGCTCTC ATTATCATCA CCTTCCAGGA GCAAGGGGAC 
AAGATGATGG AGGAGTGTAG CCTGGAGAAG AATGAGCGGG CATGCATCGA CTTCGCCATT 
AGTGCCAAAC CTCTCACCCG CTACATGCCA CAGAACAGGC ACACCTTCCA GTACCGTGTG 
TGGCACTTTG TAGTCTCCCC ATCCTTTGAG TATACCATCA TGGCCATGAT TGCCTTGAAC 
ACTGTCGTGC TGATGATGAA GTACTACTCT GCTCCTTGCA CCTATGAGCT CGCCTTAAAG 
TACCTGAACA TCGCCTTCAC TATGGTGTTT TCCCTGGAGT GTGTCCTAAA GGTCATTGCT 
TTCGGCTTCC TGAACTATTT CCGAGATACC TGGAACATCT TTGACTTCAT CACGGTGATC 
GGCAGTATCA CAGAAATTAT CCTGACAGAT AGCAAGCTGG TGAACACCAG TGGCTTCAAT 
ATGAGCTTTC TGAAGCTCTT CCGAGCTGCA CGGCTCATAA AGCTTCTGAG ACAGGGCTAC 
ACCATCCGCA TCTTACTGTG GACCTTCGTG CAGTCCTTTA AGGCTCTTCC CTACGTCTGC 
CTTCTCATTG CCATGCTTTT CTTCATCTAC GCCATCATTG GGATGCAGGT GTTTGGAAAC 
ATAAAGTTAG ATGAGGAGAG TCACATCAAC CGGCACAACA ACTTCCGGAG TTTCTTTGGA 
TCCCTCATGC TGCTTTTCAG GAGTGCCACG GGGGAGGCTT GGCAGGAGAT TATGCTGTCC 
TGTCTCGGTG AGAAGGGTTG TGAGCCTGAC ACCACTGCAC CCTCAGGGCA GAATGAGAGT 
GAGCGCTGTG GCACTGACCT GGCCTACGTT TATTTTGTCT CCTTCATCTT CTTCTGCTCC 
TTCCTGATGC TCAACCTGTT CGTGGCTGTC ATCATGGACA ACTTTGAGTA CCTGACTCGA 
GATTCCTCCA TCCTGGGGCC TCACCACTTA GATGAGTTTG TCCGTGTCTG GGCAGAATAT 
GACAGAGCAG CATGTGGCCG CATCCATTAC ACTGAGATGT ATGAAATGCT GACTCTCATG 
TCACCACCGC TAGGCCTCGG CAAGAGATGT CCCTCCAAAG TGGCCTATAA GAGGCTGGTC 
CTGATGAACA TGCCAGTGGC TGAGGACATG ACAGTCCACT TCACCTCCAC ACTTATGGCT 
CTGATCCGGA CAGCTCTGGA CATTAAAATC GCCAAAGGTG GTGCAGACAG ACAGCAGCTA 
GACTCGGAGC TGCAAAAAGA GACCCTGGCC ATTTGGCCTC ACCTGTCCCA AAAGATGTTG 
GATCTGCTTG TGCCCATGCC CAAAGCCTCT GACCTGACTG TGGGCAAGAT CTATGCAGCA 
ATGATGATCA TGGACTACTA TAAACAGAGT AAGGTAAAGA AACAGAGACA GCAGCTGGAA 
GAACAGAAAA ATGCACCCAT GTTCCAGCGC ATGGAGCCCT CATCACTGCC TCAGGAGATC 
ATTGCTAATG CCAAAGCCCT GCCTTATCTC CAGCAGGACC CTGTTTCTGG CCTGAGTGGT 
CGGAGTGGAT ACCCTTCAAT GAGTCCACTC TCCCCTCAAG AAATATTCCA GTTGGCTTGT 
ATGGACCCAG CGGATGATGG ACAATTCCAG GAACAACAAT CTCTGGTGGT AACAGACCCT 
AGCTCTATGC GACGTTCATT TTCTACAATT CGGGATAAGC GTTCAAATTC CTCCTGGTTG 
GAGGAGTTCT CCATGGAACG CAGCAGTGAA AACACTTACA AGTCGCGGCG CAGAAGTTAC 
CATTCCTCCC TGAGGCTGTC AGCACACCGC CTGAATTCTG ACTCTGGTCA CAAGTCTGAC 
ACTCACCGAT CAGGAGGCAG GGAGAGAGGC CGATCCAAAG AACGAAAGCA CCTCCTCTCT 
CCAGATGTCT CCCGCTGCAA TTCTGAAGAG CGAGGGACCC AGGCCGACTG GGAGTCCCCA 
GAACGTCGCC AGTCCAGGTC ACCCAGTGAA GGAAGGTCAC AGACCCCCAA TAGACAGGGT 
ACCGGTTCCC TAAGTGAGAG CTCCATCCCC TCTATTTCCG ACACCAGCAC CCCAAGACGA 
AGCCGTCGCC AGCTCCCACC TGTGCCACCA AAGCCTCGTC CCCTCCTCTC CTACAGCTCC 
CTGATGAGAC ACACTGGCGG CATCTCTCCA CCTCCTGATG GAAGTGAGGG TGGATCCCCA 
CTGGCCTCTC AAGCTCTGGA GAGCAACAGC GCTTGCCTGA CCGAGTCTTC CAACTCCTTG 
CACCCCCAGC AGGGCCAGCA TCCTTCCCCA CAGCACTACA TCTCCGAGCC CTATCTGGCC 
CTCCATGAAG ACTCCCACGC CTCAGACTGT GGCGAGGAGG AGACACTCAC CTTTGAGGCA 
GCCGTAGCCA CTAGCTTGGG CCGGTCCAAT ACCATTGGCT CTGCGCCACC CCTACGGCAC 
AGCTGGCAGA TGCCTAATGG GCACTATCGG CGGCGGAGGC GTGGGGGGCC TGGGCCTGGC 
ATGATGTGTG GAGCTGTCAG TGACCTCCTG AGTGACACGG AAGAAGACGA TAAGTGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cacna1e ( XM_017312795.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017168284.1 Mus musculus calcium channel, voltage-dependent, R type, alpha 1E subunit (Cacna1e), transcript variant X15, mRNA.

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CloneID	OMu27877
Clone ID Related Accession (Same CDS sequence)	XM_017312795.1
Accession Version	XM_017312795.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7203bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	voltage-dependent R-type calcium channel subunit alpha-1E isoform X12
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGCCCG AGATGCGTGG ACTGAGCCCA GGCCGAAGGC TGCCCGAGCC CACCCTGTGC 
CCGGCTACAG GAGGGAGCCC TGAGCCCGGG GCTGCTGGCA GGCGCCGCCA CGTCCTGGCT 
CTGCCTGATG AGGAGGCATG GCGGCCGCAG CAGTGCGCCC TGCTCGAGGC CAATGCCTCC 
GACGAGGTGG GCATGTTGCC TGCTTCCCCG CGCTCCGCGG TGGCCGGCTT CGACAACTTC 
TTCCCGGTGC AGGAAGGCGA GGGCCCGGGC TGGGAGGGCA GCACCACGCT GGATGCAGGC 
TCCAGCCCCT TCCTTCCTGT GAGCCCAGAG GTCATGAAGC GGAGGCGAGG GGGGCTCATC 
GAGCAGCGGG ACATCATCAA GGCCCACGAG GCACACAAGA TGCAGAGCAC CCCGCAGGCC 
CGGCGCAAGG AGTGGGAGAT GGCTCGCTTC GGGGAGGCGG TGGTCGTTGG CAGGCCAGGC 
TCAGGCGATG GAGACTCGGA CCAGAGCAGG AACCGACAAG GAACCCCCGT CCCGGCCTCG 
GGGCCGGCGG CCGCCTACAA GCAGTCAAAA GCGCAGAGGG CGCGGACTAT GGCTTTGTAC 
AACCCCATAC CAGTCCGGCA GAACTGTTTC ACGGTCAACA GATCCCTGTT CATCTTCGGA 
GAAGATAACA TTGTCAGGAA ATACGCCAAG AAGCTCATCG ATTGGCCGCC ATTTGAGTAC 
ATGATCCTGG CCACCATCAT TGCCAACTGC ATCGTCTTGG CCCTGGAGCA GCATCTTCCT 
GAGGATGACA AGACCCCAAT GTCTCGAAGA CTGGAGAAGA CAGAACCATA TTTCATTGGG 
ATCTTCTGCT TTGAAGCTGG GATCAAAATT GTGGCTCTAG GGTTCATCTT CCATAAGGGT 
TCATACCTCC GTAATGGCTG GAATGTCATG GACTTCATCG TGGTCCTTAG TGGCATCCTG 
GCCACTGCGG GAACCCACTT CAACACCCAC GTGGACCTGC GGACCCTCCG GGCTGTGCGT 
GTCCTGAGGC CTTTAAAGCT GGTATCAGGA ATACCTAGCC TGCAGATCGT GCTGAAGTCT 
ATCATGAAGG CCATGGTGCC TCTTCTGCAG ATTGGCCTTC TGCTCTTCTT TGCCATCCTC 
ATGTTTGCTA TCATTGGCTT GGAGTTCTAC AGTGGCAAGT TGCATCGAGC ATGCTTCATG 
AACAATTCAG GTATTCTAGA GGGGTTTGAT CCTCCTCACC CGTGTGGTGT GCAAGGCTGC 
CCGGCTGGTT ATGAATGTAA GGACTGGATC GGCCCCAATG ACGGGATCAC CCAGTTTGAC 
AACATCCTTT TTGCCGTGCT GACTGTCTTC CAATGCATCA CTATGGAAGG GTGGACCACT 
GTGCTGTACA ATACCAATGA TGCCTTAGGA GCCACCTGGA ACTGGCTGTA CTTCATCCCT 
CTCATCATCA TCGGATCCTT CTTTGTTCTC AACCTCGTCC TGGGAGTGCT TTCCGGGGAA 
TTTGCCAAAG AAAGAGAGAG AGTGGAGAAC CGAAGAGCGT TCATGAAGCT CCGGCGCCAG 
CAACAGATTG AGCGAGAATT GAACGGCTAC CGTGCCTGGA TAGACAAAGC AGAGGAAGTC 
ATGCTTGCTG AAGAAAATAA AAACTCGGGA ACATCAGCCT TGGAAGTGCT TCGAAGGGCA 
ACCATCAAAA GGAGCCGGAC GGAGGCCATG ACCCGAGACT CCAGCGATGA GCACTGCGTT 
GATATCTCTT CAGTGGGCAC ACCTCTTGCC AGAGCCAGTA TCAAGAGCAC AAAAGTAGAT 
GGGGCCTCCT ATTTCCGGCA CAAGGAACGG CTTCTGCGCA TCTCTATCCG CCACATGGTC 
AAGTCCCAAG TCTTTTACTG GATCGTCCTG AGTGTTGTGG CTCTTAATAC TGCCTGTGTG 
GCCATTGTTC ATCACAACCA GCCACAGTGG CTCACTCACC TCCTCTACTA TGCAGAGTTT 
TTGTTTCTGG GACTCTTCCT GTTGGAGATG TCCCTGAAGA TGTATGGCAT GGGGCCACGC 
CTCTATTTTC ACTCATCCTT CAACTGCTTT GATTTTGGGG TCACAGTGGG CAGCATCTTT 
GAGGTTGTCT GGGCAATCTT CAGACCTGGC ACATCTTTTG GAATCAGTGT CTTACGAGCT 
CTCCGGCTCC TAAGGATATT TAAAATAACC AAGTATTGGG CATCCCTCCG GAATCTGGTT 
GTCTCCTTGA TGAGCTCCAT GAAGTCTATC ATCAGCTTAC TCTTCCTTCT CTTCCTCTTC 
ATCGTTGTCT TTGCTCTCCT GGGAATGCAG CTCTTTGGAG GCAGGTTTAA CTTTAATGAT 
GGGACTCCTT CAGCTAATTT TGACACCTTC CCTGCAGCTA TCATGACAGT GTTCCAGATC 
CTGACAGGGG AAGACTGGAA TGAGGTGATG TACAATGGCA TTCGCTCCCA GGGTGGGGTC 
AGCTCAGGCA TGTGGTCTGC CATCTACTTC ATTGTGCTCA CCTTGTTTGG AAACTACACG 
CTGCTAAATG TATTCTTGGC CATCGCTGTG GACAATCTGG CCAATGCCCA GGAACTGACC 
AAGGATGAGC AGGAGGAAGA AGAAGCCTTC AACCAGAAAC ATGCACTGCA GAAGGCCAAG 
GAGGTCAGCC CGATGTCTGC TCCCAACATG CCTTCCATTG AGAGGGAGCG GAGGCGCCGG 
CACCACATGT CTGTGTGGGA ACAACGCACC AGCCAGCTGA GGAGGCACAT GCAAATGTCT 
AGCCAAGAAG CCCTCAACAA AGAGGAGGCC CCGCCAATGA ACCCCCTCAA TCCACTCAAC 
CCACTGAGTC CTCTCAACCC CCTCAATGCT CACCCCAGCC TTTATCGGAG GCCCAGGCCC 
ATTGAGGGCC TAGCCCTAGG TCTGGGTCTT GAAAAGTGTG AAGAAGAACG CATCAGTCGT 
GGGGGCTCCC TCAAGGGGGA CATAGGGGGC CTGACTAGCG CCCTGGACAA CCAGAGGAGC 
CCCTTGTCCT TGGGCAAACG GGAGCCACCG TGGCTGCCTA GATCCTGTCA TGGAAACTGT 
GACCCAATCC AGCAGGAGGC TGGGGGAGGA GAGACTGTGG TGACTTTTGA GGACCGGGCC 
AGGCACAGGC AGAGTCAGAG GCGAAGCCGG CATCGCCGAG TCAGGACTGA GGGCAAGGAC 
TCTGCCTCTG CATCCCGAAG CAGGTCTGCC AGCCAGGAGC GGAGTCTGGA TGAAGGGGTG 
TCCGTTGAGG GGGAGAAGGA ACATGAGCCT CACAGCAGCC ACAGGAGCAA AGAGCCGACC 
ATCCACGAAG AGGAGAGAAC CCAGGACTTA AGGAGGACCA ACAGTCTGAT GGTCCCCAGG 
GGCTCTGGAC TGGTAGGGGC TCTTGATGAA GCAGAAACAC CTCTAGTCCA GCCCCAACCT 
GAGCTGGAAG TGGGGAAGGA TGCAGCTCTG ACAGAGCAGG AGGCTGAAGG CAGCAGTGAA 
CAAGCCCTTC TTGGGGACGT ACAGCTGGAT GTGGGCCGGG GCATTAGCCA GAGTGAACCG 
GATCTCTCCT GCATGACAGC CAACATGGAC AAGGCTACCA CGGAGAGCAC CAGTGTCACG 
GTCGCCATCC CTGATGTGGA CCCCTTGGTG GACTCGACAG TGGTGAACAT TAGCAACAAG 
ACGGATGGAG AAGCCAGTCC CTTGAAGGAG GCAGAGACTA AAGAGGAAGA AGAAGAGGTA 
GAGAAGAAGA AGAAGCAGAA GAAGGAGAAG CGAGAGACTG GAAAAGCCAT GGTGCCCCAT 
AGCTCTATGT TCATCTTCAG CACCACTAAC CCGATCCGCA GGGCCTGCCA CTACATTGTG 
AACCTACGCT ACTTTGAAAT GTGCATTCTC TTGGTGATTG CGGCCAGCAG CATTGCCCTG 
GCAGCCGAGG ACCCTGTACT AACTAACTCG GAGCGTAACA AAGTCCTGAG ATATTTCGAC 
TATGTTTTCA CAGGAGTGTT TACCTTTGAG ATGGTTATAA AGATGATAGA CCAAGGCTTG 
ATCCTGCAGG ATGGATCATA CTTCAGAGAC CTCTGGAACA TCCTGGACTT TGTAGTAGTG 
GTTGGTGCAT TGGTAGCATT TGCTCTGGCG AACGCTTTGG GAACCAACAA GGGGAGGGAT 
ATCAAGACTA TCAAGTCTCT GAGGGTACTT CGAGTTCTGC GGCCACTGAA AACCATCAAG 
CGCTTGCCCA AGCTGAAGGC TGTGTTTGAC TGCGTGGTGA CCTCCTTGAA GAATGTCTTC 
AACATACTCA TTGTCTACAA GCTCTTCATG TTCATCTTTG CCGTCATCGC TGTTCAGCTA 
TTCAAAGGAA AGTTCTTTTA TTGCACTGAC AGTTCCAAGG ACACAGAGAA GGAGTGCATA 
GGCAACTATG TAGACCACGA GAAAAACAAG ATGGAGGTGA AGGGCCGGGA ATGGAAGCGT 
CATGAATTCC ACTATGACAA CATCATCTGG GCCCTGCTTA CCCTCTTCAC AGTCTCTACA 
GGGGAAGGAT GGCCTCAGGT TCTGCAGCAC TCTGTAGATG TGACAGAGGA GGACAGAGGC 
CCAAGTCGCA GCAACCGCAT GGAGATGTCC ATCTTTTATG TGGTCTACTT TGTGGTCTTC 
CCTTTCTTCT TTGTCAATAT CTTCGTGGCT CTCATTATCA TCACCTTCCA GGAGCAAGGG 
GACAAGATGA TGGAGGAGTG TAGCCTGGAG AAGAATGAGC GGGCATGCAT CGACTTCGCC 
ATTAGTGCCA AACCTCTCAC CCGCTACATG CCACAGAACA GGCACACCTT CCAGTACCGT 
GTGTGGCACT TTGTAGTCTC CCCATCCTTT GAGTATACCA TCATGGCCAT GATTGCCTTG 
AACACTGTCG TGCTGATGAT GAAGTACTAC TCTGCTCCTT GCACCTATGA GCTCGCCTTA 
AAGTACCTGA ACATCGCCTT CACTATGGTG TTTTCCCTGG AGTGTGTCCT AAAGGTCATT 
GCTTTCGGCT TCCTGAACTA TTTCCGAGAT ACCTGGAACA TCTTTGACTT CATCACGGTG 
ATCGGCAGTA TCACAGAAAT TATCCTGACA GATAGCAAGC TGGTGAACAC CAGTGGCTTC 
AATATGAGCT TTCTGAAGCT CTTCCGAGCT GCACGGCTCA TAAAGCTTCT GAGACAGGGC 
TACACCATCC GCATCTTACT GTGGACCTTC GTGCAGTCCT TTAAGGCTCT TCCCTACGTC 
TGCCTTCTCA TTGCCATGCT TTTCTTCATC TACGCCATCA TTGGGATGCA GGTGTTTGGA 
AACATAAAGT TAGATGAGGA GAGTCACATC AACCGGCACA ACAACTTCCG GAGTTTCTTT 
GGATCCCTCA TGCTGCTTTT CAGGAGTGCC ACGGGGGAGG CTTGGCAGGA GATTATGCTG 
TCCTGTCTCG GTGAGAAGGG TTGTGAGCCT GACACCACTG CACCCTCAGG GCAGAATGAG 
AGTGAGCGCT GTGGCACTGA CCTGGCCTAC GTTTATTTTG TCTCCTTCAT CTTCTTCTGC 
TCCTTCCTGA TGCTCAACCT GTTCGTGGCT GTCATCATGG ACAACTTTGA GTACCTGACT 
CGAGATTCCT CCATCCTGGG GCCTCACCAC TTAGATGAGT TTGTCCGTGT CTGGGCAGAA 
TATGACAGAG CAGCATGTGG CCGCATCCAT TACACTGAGA TGTATGAAAT GCTGACTCTC 
ATGTCACCAC CGCTAGGCCT CGGCAAGAGA TGTCCCTCCA AAGTGGCCTA TAAGAGGCTG 
GTCCTGATGA ACATGCCAGT GGCTGAGGAC ATGACAGTCC ACTTCACCTC CACACTTATG 
GCTCTGATCC GGACAGCTCT GGACATTAAA ATCGCCAAAG GTGGTGCAGA CAGACAGCAG 
CTAGACTCGG AGCTGCAAAA AGAGACCCTG GCCATTTGGC CTCACCTGTC CCAAAAGATG 
TTGGATCTGC TTGTGCCCAT GCCCAAAGCC TCTGACCTGA CTGTGGGCAA GATCTATGCA 
GCAATGATGA TCATGGACTA CTATAAACAG AGTAAGGTAA AGAAACAGAG ACAGCAGCTG 
GAAGAACAGA AAAATGCACC CATGTTCCAG CGCATGGAGC CCTCATCACT GCCTCAGGAG 
ATCATTGCTA ATGCCAAAGC CCTGCCTTAT CTCCAGCAGG ACCCTGTTTC TGGCCTGAGT 
GGTCGGAGTG GATACCCTTC AATGAGTCCA CTCTCCCCTC AAGAAATATT CCAGTTGGCT 
TGTATGGACC CAGCGGATGA TGGACAATTC CAGGAACAAC AATCTCTGGT GGTAACAGAC 
CCTAGCTCTA TGCGACGTTC ATTTTCTACA ATTCGGGATA AGCGTTCAAA TTCCTCCTGG 
TTGGAGGAGT TCTCCATGGA ACGCAGCAGT GAAAACACTT ACAAGTCGCG GCGCAGAAGT 
TACCATTCCT CCCTGAGGCT GTCAGCACAC CGCCTGAATT CTGACTCTGG TCACAAGTCT 
GACACTCACC GATCAGGAGG CAGGGAGAGA GGCCGATCCA AAGAACGAAA GCACCTCCTC 
TCTCCAGATG TCTCCCGCTG CAATTCTGAA GAGCGAGGGA CCCAGGCCGA CTGGGAGTCC 
CCAGAACGTC GCCAGTCCAG GTCACCCAGT GAAGGAAGGT CACAGACCCC CAATAGACAG 
GGTACCGGTT CCCTAAGTGA GAGCTCCATC CCCTCTATTT CCGACACCAG CACCCCAAGA 
CGAAGCCGTC GCCAGCTCCC ACCTGTGCCA CCAAAGCCTC GTCCCCTCCT CTCCTACAGC 
TCCCTGATGA GACACACTGG CGGCATCTCT CCACCTCCTG ATGGAAGTGA GGGTGGATCC 
CCACTGGCCT CTCAAGCTCT GGAGAGCAAC AGCGCTTGCC TGACCGAGTC TTCCAACTCC 
TTGCACCCCC AGCAGGGCCA GCATCCTTCC CCACAGCACT ACATCTCCGA GCCCTATCTG 
GCCCTCCATG AAGACTCCCA CGCCTCAGAC TGTGGCGAGG AGGAGACACT CACCTTTGAG 
GCAGCCGTAG CCACTAGCTT GGGCCGGTCC AATACCATTG GCTCTGCGCC ACCCCTACGG 
CACAGCTGGC AGATGCCTAA TGGGCACTAT CGGCGGCGGA GGCGTGGGGG GCCTGGGCCT 
GGCATGATGT GTGGAGCTGT CAGTGACCTC CTGAGTGACA CGGAAGAAGA CGATAAGTGC 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017168284.1
CDS	594..7796
Translation	MVPEMRGLSPGRRLPEPTLCPATGGSPEPGAAGRRRHVLALPDEEAWRPQQCALLEANASDEVGMLPASPRSAVAGFDNFFPVQEGEGPGWEGSTTLDAGSSPFLPVSPEVMKRRRGGLIEQRDIIKAHEAHKMQSTPQARRKEWEMARFGEAVVVGRPGSGDGDSDQSRNRQGTPVPASGPAAAYKQSKAQRARTMALYNPIPVRQNCFTVNRSLFIFGEDNIVRKYAKKLIDWPPFEYMILATIIANCIVLALEQHLPEDDKTPMSRRLEKTEPYFIGIFCFEAGIKIVALGFIFHKGSYLRNGWNVMDFIVVLSGILATAGTHFNTHVDLRTLRAVRVLRPLKLVSGIPSLQIVLKSIMKAMVPLLQIGLLLFFAILMFAIIGLEFYSGKLHRACFMNNSGILEGFDPPHPCGVQGCPAGYECKDWIGPNDGITQFDNILFAVLTVFQCITMEGWTTVLYNTNDALGATWNWLYFIPLIIIGSFFVLNLVLGVLSGEFAKERERVENRRAFMKLRRQQQIERELNGYRAWIDKAEEVMLAEENKNSGTSALEVLRRATIKRSRTEAMTRDSSDEHCVDISSVGTPLARASIKSTKVDGASYFRHKERLLRISIRHMVKSQVFYWIVLSVVALNTACVAIVHHNQPQWLTHLLYYAEFLFLGLFLLEMSLKMYGMGPRLYFHSSFNCFDFGVTVGSIFEVVWAIFRPGTSFGISVLRALRLLRIFKITKYWASLRNLVVSLMSSMKSIISLLFLLFLFIVVFALLGMQLFGGRFNFNDGTPSANFDTFPAAIMTVFQILTGEDWNEVMYNGIRSQGGVSSGMWSAIYFIVLTLFGNYTLLNVFLAIAVDNLANAQELTKDEQEEEEAFNQKHALQKAKEVSPMSAPNMPSIERERRRRHHMSVWEQRTSQLRRHMQMSSQEALNKEEAPPMNPLNPLNPLSPLNPLNAHPSLYRRPRPIEGLALGLGLEKCEEERISRGGSLKGDIGGLTSALDNQRSPLSLGKREPPWLPRSCHGNCDPIQQEAGGGETVVTFEDRARHRQSQRRSRHRRVRTEGKDSASASRSRSASQERSLDEGVSVEGEKEHEPHSSHRSKEPTIHEEERTQDLRRTNSLMVPRGSGLVGALDEAETPLVQPQPELEVGKDAALTEQEAEGSSEQALLGDVQLDVGRGISQSEPDLSCMTANMDKATTESTSVTVAIPDVDPLVDSTVVNISNKTDGEASPLKEAETKEEEEEVEKKKKQKKEKRETGKAMVPHSSMFIFSTTNPIRRACHYIVNLRYFEMCILLVIAASSIALAAEDPVLTNSERNKVLRYFDYVFTGVFTFEMVIKMIDQGLILQDGSYFRDLWNILDFVVVVGALVAFALANALGTNKGRDIKTIKSLRVLRVLRPLKTIKRLPKLKAVFDCVVTSLKNVFNILIVYKLFMFIFAVIAVQLFKGKFFYCTDSSKDTEKECIGNYVDHEKNKMEVKGREWKRHEFHYDNIIWALLTLFTVSTGEGWPQVLQHSVDVTEEDRGPSRSNRMEMSIFYVVYFVVFPFFFVNIFVALIIITFQEQGDKMMEECSLEKNERACIDFAISAKPLTRYMPQNRHTFQYRVWHFVVSPSFEYTIMAMIALNTVVLMMKYYSAPCTYELALKYLNIAFTMVFSLECVLKVIAFGFLNYFRDTWNIFDFITVIGSITEIILTDSKLVNTSGFNMSFLKLFRAARLIKLLRQGYTIRILLWTFVQSFKALPYVCLLIAMLFFIYAIIGMQVFGNIKLDEESHINRHNNFRSFFGSLMLLFRSATGEAWQEIMLSCLGEKGCEPDTTAPSGQNESERCGTDLAYVYFVSFIFFCSFLMLNLFVAVIMDNFEYLTRDSSILGPHHLDEFVRVWAEYDRAACGRIHYTEMYEMLTLMSPPLGLGKRCPSKVAYKRLVLMNMPVAEDMTVHFTSTLMALIRTALDIKIAKGGADRQQLDSELQKETLAIWPHLSQKMLDLLVPMPKASDLTVGKIYAAMMIMDYYKQSKVKKQRQQLEEQKNAPMFQRMEPSSLPQEIIANAKALPYLQQDPVSGLSGRSGYPSMSPLSPQEIFQLACMDPADDGQFQEQQSLVVTDPSSMRRSFSTIRDKRSNSSWLEEFSMERSSENTYKSRRRSYHSSLRLSAHRLNSDSGHKSDTHRSGGRERGRSKERKHLLSPDVSRCNSEERGTQADWESPERRQSRSPSEGRSQTPNRQGTGSLSESSIPSISDTSTPRRSRRQLPPVPPKPRPLLSYSSLMRHTGGISPPPDGSEGGSPLASQALESNSACLTESSNSLHPQQGQHPSPQHYISEPYLALHEDSHASDCGEEETLTFEAAVATSLGRSNTIGSAPPLRHSWQMPNGHYRRRRRGGPGPGMMCGAVSDLLSDTEEDDKC

Target ORF information:

RefSeq Version	XM_017312795.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus calcium channel, voltage-dependent, R type, alpha 1E subunit (Cacna1e), transcript variant X15, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017312795.1

ORF Insert Sequence:

ATGGTGCCCG AGATGCGTGG ACTGAGCCCA GGCCGAAGGC TGCCCGAGCC CACCCTGTGC 
CCGGCTACAG GAGGGAGCCC TGAGCCCGGG GCTGCTGGCA GGCGCCGCCA CGTCCTGGCT 
CTGCCTGATG AGGAGGCATG GCGGCCGCAG CAGTGCGCCC TGCTCGAGGC CAATGCCTCC 
GACGAGGTGG GCATGTTGCC TGCTTCCCCG CGCTCCGCGG TGGCCGGCTT CGACAACTTC 
TTCCCGGTGC AGGAAGGCGA GGGCCCGGGC TGGGAGGGCA GCACCACGCT GGATGCAGGC 
TCCAGCCCCT TCCTTCCTGT GAGCCCAGAG GTCATGAAGC GGAGGCGAGG GGGGCTCATC 
GAGCAGCGGG ACATCATCAA GGCCCACGAG GCACACAAGA TGCAGAGCAC CCCGCAGGCC 
CGGCGCAAGG AGTGGGAGAT GGCTCGCTTC GGGGAGGCGG TGGTCGTTGG CAGGCCAGGC 
TCAGGCGATG GAGACTCGGA CCAGAGCAGG AACCGACAAG GAACCCCCGT CCCGGCCTCG 
GGGCCGGCGG CCGCCTACAA GCAGTCAAAA GCGCAGAGGG CGCGGACTAT GGCTTTGTAC 
AACCCCATAC CAGTCCGGCA GAACTGTTTC ACGGTCAACA GATCCCTGTT CATCTTCGGA 
GAAGATAACA TTGTCAGGAA ATACGCCAAG AAGCTCATCG ATTGGCCGCC ATTTGAGTAC 
ATGATCCTGG CCACCATCAT TGCCAACTGC ATCGTCTTGG CCCTGGAGCA GCATCTTCCT 
GAGGATGACA AGACCCCAAT GTCTCGAAGA CTGGAGAAGA CAGAACCATA TTTCATTGGG 
ATCTTCTGCT TTGAAGCTGG GATCAAAATT GTGGCTCTAG GGTTCATCTT CCATAAGGGT 
TCATACCTCC GTAATGGCTG GAATGTCATG GACTTCATCG TGGTCCTTAG TGGCATCCTG 
GCCACTGCGG GAACCCACTT CAACACCCAC GTGGACCTGC GGACCCTCCG GGCTGTGCGT 
GTCCTGAGGC CTTTAAAGCT GGTATCAGGA ATACCTAGCC TGCAGATCGT GCTGAAGTCT 
ATCATGAAGG CCATGGTGCC TCTTCTGCAG ATTGGCCTTC TGCTCTTCTT TGCCATCCTC 
ATGTTTGCTA TCATTGGCTT GGAGTTCTAC AGTGGCAAGT TGCATCGAGC ATGCTTCATG 
AACAATTCAG GTATTCTAGA GGGGTTTGAT CCTCCTCACC CGTGTGGTGT GCAAGGCTGC 
CCGGCTGGTT ATGAATGTAA GGACTGGATC GGCCCCAATG ACGGGATCAC CCAGTTTGAC 
AACATCCTTT TTGCCGTGCT GACTGTCTTC CAATGCATCA CTATGGAAGG GTGGACCACT 
GTGCTGTACA ATACCAATGA TGCCTTAGGA GCCACCTGGA ACTGGCTGTA CTTCATCCCT 
CTCATCATCA TCGGATCCTT CTTTGTTCTC AACCTCGTCC TGGGAGTGCT TTCCGGGGAA 
TTTGCCAAAG AAAGAGAGAG AGTGGAGAAC CGAAGAGCGT TCATGAAGCT CCGGCGCCAG 
CAACAGATTG AGCGAGAATT GAACGGCTAC CGTGCCTGGA TAGACAAAGC AGAGGAAGTC 
ATGCTTGCTG AAGAAAATAA AAACTCGGGA ACATCAGCCT TGGAAGTGCT TCGAAGGGCA 
ACCATCAAAA GGAGCCGGAC GGAGGCCATG ACCCGAGACT CCAGCGATGA GCACTGCGTT 
GATATCTCTT CAGTGGGCAC ACCTCTTGCC AGAGCCAGTA TCAAGAGCAC AAAAGTAGAT 
GGGGCCTCCT ATTTCCGGCA CAAGGAACGG CTTCTGCGCA TCTCTATCCG CCACATGGTC 
AAGTCCCAAG TCTTTTACTG GATCGTCCTG AGTGTTGTGG CTCTTAATAC TGCCTGTGTG 
GCCATTGTTC ATCACAACCA GCCACAGTGG CTCACTCACC TCCTCTACTA TGCAGAGTTT 
TTGTTTCTGG GACTCTTCCT GTTGGAGATG TCCCTGAAGA TGTATGGCAT GGGGCCACGC 
CTCTATTTTC ACTCATCCTT CAACTGCTTT GATTTTGGGG TCACAGTGGG CAGCATCTTT 
GAGGTTGTCT GGGCAATCTT CAGACCTGGC ACATCTTTTG GAATCAGTGT CTTACGAGCT 
CTCCGGCTCC TAAGGATATT TAAAATAACC AAGTATTGGG CATCCCTCCG GAATCTGGTT 
GTCTCCTTGA TGAGCTCCAT GAAGTCTATC ATCAGCTTAC TCTTCCTTCT CTTCCTCTTC 
ATCGTTGTCT TTGCTCTCCT GGGAATGCAG CTCTTTGGAG GCAGGTTTAA CTTTAATGAT 
GGGACTCCTT CAGCTAATTT TGACACCTTC CCTGCAGCTA TCATGACAGT GTTCCAGATC 
CTGACAGGGG AAGACTGGAA TGAGGTGATG TACAATGGCA TTCGCTCCCA GGGTGGGGTC 
AGCTCAGGCA TGTGGTCTGC CATCTACTTC ATTGTGCTCA CCTTGTTTGG AAACTACACG 
CTGCTAAATG TATTCTTGGC CATCGCTGTG GACAATCTGG CCAATGCCCA GGAACTGACC 
AAGGATGAGC AGGAGGAAGA AGAAGCCTTC AACCAGAAAC ATGCACTGCA GAAGGCCAAG 
GAGGTCAGCC CGATGTCTGC TCCCAACATG CCTTCCATTG AGAGGGAGCG GAGGCGCCGG 
CACCACATGT CTGTGTGGGA ACAACGCACC AGCCAGCTGA GGAGGCACAT GCAAATGTCT 
AGCCAAGAAG CCCTCAACAA AGAGGAGGCC CCGCCAATGA ACCCCCTCAA TCCACTCAAC 
CCACTGAGTC CTCTCAACCC CCTCAATGCT CACCCCAGCC TTTATCGGAG GCCCAGGCCC 
ATTGAGGGCC TAGCCCTAGG TCTGGGTCTT GAAAAGTGTG AAGAAGAACG CATCAGTCGT 
GGGGGCTCCC TCAAGGGGGA CATAGGGGGC CTGACTAGCG CCCTGGACAA CCAGAGGAGC 
CCCTTGTCCT TGGGCAAACG GGAGCCACCG TGGCTGCCTA GATCCTGTCA TGGAAACTGT 
GACCCAATCC AGCAGGAGGC TGGGGGAGGA GAGACTGTGG TGACTTTTGA GGACCGGGCC 
AGGCACAGGC AGAGTCAGAG GCGAAGCCGG CATCGCCGAG TCAGGACTGA GGGCAAGGAC 
TCTGCCTCTG CATCCCGAAG CAGGTCTGCC AGCCAGGAGC GGAGTCTGGA TGAAGGGGTG 
TCCGTTGAGG GGGAGAAGGA ACATGAGCCT CACAGCAGCC ACAGGAGCAA AGAGCCGACC 
ATCCACGAAG AGGAGAGAAC CCAGGACTTA AGGAGGACCA ACAGTCTGAT GGTCCCCAGG 
GGCTCTGGAC TGGTAGGGGC TCTTGATGAA GCAGAAACAC CTCTAGTCCA GCCCCAACCT 
GAGCTGGAAG TGGGGAAGGA TGCAGCTCTG ACAGAGCAGG AGGCTGAAGG CAGCAGTGAA 
CAAGCCCTTC TTGGGGACGT ACAGCTGGAT GTGGGCCGGG GCATTAGCCA GAGTGAACCG 
GATCTCTCCT GCATGACAGC CAACATGGAC AAGGCTACCA CGGAGAGCAC CAGTGTCACG 
GTCGCCATCC CTGATGTGGA CCCCTTGGTG GACTCGACAG TGGTGAACAT TAGCAACAAG 
ACGGATGGAG AAGCCAGTCC CTTGAAGGAG GCAGAGACTA AAGAGGAAGA AGAAGAGGTA 
GAGAAGAAGA AGAAGCAGAA GAAGGAGAAG CGAGAGACTG GAAAAGCCAT GGTGCCCCAT 
AGCTCTATGT TCATCTTCAG CACCACTAAC CCGATCCGCA GGGCCTGCCA CTACATTGTG 
AACCTACGCT ACTTTGAAAT GTGCATTCTC TTGGTGATTG CGGCCAGCAG CATTGCCCTG 
GCAGCCGAGG ACCCTGTACT AACTAACTCG GAGCGTAACA AAGTCCTGAG ATATTTCGAC 
TATGTTTTCA CAGGAGTGTT TACCTTTGAG ATGGTTATAA AGATGATAGA CCAAGGCTTG 
ATCCTGCAGG ATGGATCATA CTTCAGAGAC CTCTGGAACA TCCTGGACTT TGTAGTAGTG 
GTTGGTGCAT TGGTAGCATT TGCTCTGGCG AACGCTTTGG GAACCAACAA GGGGAGGGAT 
ATCAAGACTA TCAAGTCTCT GAGGGTACTT CGAGTTCTGC GGCCACTGAA AACCATCAAG 
CGCTTGCCCA AGCTGAAGGC TGTGTTTGAC TGCGTGGTGA CCTCCTTGAA GAATGTCTTC 
AACATACTCA TTGTCTACAA GCTCTTCATG TTCATCTTTG CCGTCATCGC TGTTCAGCTA 
TTCAAAGGAA AGTTCTTTTA TTGCACTGAC AGTTCCAAGG ACACAGAGAA GGAGTGCATA 
GGCAACTATG TAGACCACGA GAAAAACAAG ATGGAGGTGA AGGGCCGGGA ATGGAAGCGT 
CATGAATTCC ACTATGACAA CATCATCTGG GCCCTGCTTA CCCTCTTCAC AGTCTCTACA 
GGGGAAGGAT GGCCTCAGGT TCTGCAGCAC TCTGTAGATG TGACAGAGGA GGACAGAGGC 
CCAAGTCGCA GCAACCGCAT GGAGATGTCC ATCTTTTATG TGGTCTACTT TGTGGTCTTC 
CCTTTCTTCT TTGTCAATAT CTTCGTGGCT CTCATTATCA TCACCTTCCA GGAGCAAGGG 
GACAAGATGA TGGAGGAGTG TAGCCTGGAG AAGAATGAGC GGGCATGCAT CGACTTCGCC 
ATTAGTGCCA AACCTCTCAC CCGCTACATG CCACAGAACA GGCACACCTT CCAGTACCGT 
GTGTGGCACT TTGTAGTCTC CCCATCCTTT GAGTATACCA TCATGGCCAT GATTGCCTTG 
AACACTGTCG TGCTGATGAT GAAGTACTAC TCTGCTCCTT GCACCTATGA GCTCGCCTTA 
AAGTACCTGA ACATCGCCTT CACTATGGTG TTTTCCCTGG AGTGTGTCCT AAAGGTCATT 
GCTTTCGGCT TCCTGAACTA TTTCCGAGAT ACCTGGAACA TCTTTGACTT CATCACGGTG 
ATCGGCAGTA TCACAGAAAT TATCCTGACA GATAGCAAGC TGGTGAACAC CAGTGGCTTC 
AATATGAGCT TTCTGAAGCT CTTCCGAGCT GCACGGCTCA TAAAGCTTCT GAGACAGGGC 
TACACCATCC GCATCTTACT GTGGACCTTC GTGCAGTCCT TTAAGGCTCT TCCCTACGTC 
TGCCTTCTCA TTGCCATGCT TTTCTTCATC TACGCCATCA TTGGGATGCA GGTGTTTGGA 
AACATAAAGT TAGATGAGGA GAGTCACATC AACCGGCACA ACAACTTCCG GAGTTTCTTT 
GGATCCCTCA TGCTGCTTTT CAGGAGTGCC ACGGGGGAGG CTTGGCAGGA GATTATGCTG 
TCCTGTCTCG GTGAGAAGGG TTGTGAGCCT GACACCACTG CACCCTCAGG GCAGAATGAG 
AGTGAGCGCT GTGGCACTGA CCTGGCCTAC GTTTATTTTG TCTCCTTCAT CTTCTTCTGC 
TCCTTCCTGA TGCTCAACCT GTTCGTGGCT GTCATCATGG ACAACTTTGA GTACCTGACT 
CGAGATTCCT CCATCCTGGG GCCTCACCAC TTAGATGAGT TTGTCCGTGT CTGGGCAGAA 
TATGACAGAG CAGCATGTGG CCGCATCCAT TACACTGAGA TGTATGAAAT GCTGACTCTC 
ATGTCACCAC CGCTAGGCCT CGGCAAGAGA TGTCCCTCCA AAGTGGCCTA TAAGAGGCTG 
GTCCTGATGA ACATGCCAGT GGCTGAGGAC ATGACAGTCC ACTTCACCTC CACACTTATG 
GCTCTGATCC GGACAGCTCT GGACATTAAA ATCGCCAAAG GTGGTGCAGA CAGACAGCAG 
CTAGACTCGG AGCTGCAAAA AGAGACCCTG GCCATTTGGC CTCACCTGTC CCAAAAGATG 
TTGGATCTGC TTGTGCCCAT GCCCAAAGCC TCTGACCTGA CTGTGGGCAA GATCTATGCA 
GCAATGATGA TCATGGACTA CTATAAACAG AGTAAGGTAA AGAAACAGAG ACAGCAGCTG 
GAAGAACAGA AAAATGCACC CATGTTCCAG CGCATGGAGC CCTCATCACT GCCTCAGGAG 
ATCATTGCTA ATGCCAAAGC CCTGCCTTAT CTCCAGCAGG ACCCTGTTTC TGGCCTGAGT 
GGTCGGAGTG GATACCCTTC AATGAGTCCA CTCTCCCCTC AAGAAATATT CCAGTTGGCT 
TGTATGGACC CAGCGGATGA TGGACAATTC CAGGAACAAC AATCTCTGGT GGTAACAGAC 
CCTAGCTCTA TGCGACGTTC ATTTTCTACA ATTCGGGATA AGCGTTCAAA TTCCTCCTGG 
TTGGAGGAGT TCTCCATGGA ACGCAGCAGT GAAAACACTT ACAAGTCGCG GCGCAGAAGT 
TACCATTCCT CCCTGAGGCT GTCAGCACAC CGCCTGAATT CTGACTCTGG TCACAAGTCT 
GACACTCACC GATCAGGAGG CAGGGAGAGA GGCCGATCCA AAGAACGAAA GCACCTCCTC 
TCTCCAGATG TCTCCCGCTG CAATTCTGAA GAGCGAGGGA CCCAGGCCGA CTGGGAGTCC 
CCAGAACGTC GCCAGTCCAG GTCACCCAGT GAAGGAAGGT CACAGACCCC CAATAGACAG 
GGTACCGGTT CCCTAAGTGA GAGCTCCATC CCCTCTATTT CCGACACCAG CACCCCAAGA 
CGAAGCCGTC GCCAGCTCCC ACCTGTGCCA CCAAAGCCTC GTCCCCTCCT CTCCTACAGC 
TCCCTGATGA GACACACTGG CGGCATCTCT CCACCTCCTG ATGGAAGTGA GGGTGGATCC 
CCACTGGCCT CTCAAGCTCT GGAGAGCAAC AGCGCTTGCC TGACCGAGTC TTCCAACTCC 
TTGCACCCCC AGCAGGGCCA GCATCCTTCC CCACAGCACT ACATCTCCGA GCCCTATCTG 
GCCCTCCATG AAGACTCCCA CGCCTCAGAC TGTGGCGAGG AGGAGACACT CACCTTTGAG 
GCAGCCGTAG CCACTAGCTT GGGCCGGTCC AATACCATTG GCTCTGCGCC ACCCCTACGG 
CACAGCTGGC AGATGCCTAA TGGGCACTAT CGGCGGCGGA GGCGTGGGGG GCCTGGGCCT 
GGCATGATGT GTGGAGCTGT CAGTGACCTC CTGAGTGACA CGGAAGAAGA CGATAAGTGC 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cacna1e ( XM_017312824.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017168313.1 Mus musculus calcium channel, voltage-dependent, R type, alpha 1E subunit (Cacna1e), transcript variant X16, mRNA.

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CloneID	OMu27878
Clone ID Related Accession (Same CDS sequence)	XM_017312824.1
Accession Version	XM_017312824.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7203bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	voltage-dependent R-type calcium channel subunit alpha-1E isoform X13
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGCCCG AGATGCGTGG ACTGAGCCCA GGCCGAAGGC TGCCCGAGCC CACCCTGTGC 
CCGGCTACAG GAGGGAGCCC TGAGCCCGGG GCTGCTGGCA GGCGCCGCCA CGTCCTGGCT 
CTGCCTGATG AGGAGGCATG GCGGCCGCAG CAGTGCGCCC TGCTCGAGGC CAATGCCTCC 
GACGAGGTGG GCATGTTGCC TGCTTCCCCG CGCTCCGCGG TGGCCGGCTT CGACAACTTC 
TTCCCGGTGC AGGAAGGCGA GGGCCCGGGC TGGGAGGGCA GCACCACGCT GGATGCAGGC 
TCCAGCCCCT TCCTTCCTGT GAGCCCAGAG GTCATGAAGC GGAGGCGAGG GGGGCTCATC 
GAGCAGCGGG ACATCATCAA GGCCCACGAG GCACACAAGA TGCAGAGCAC CCCGCAGGCC 
CGGCGCAAGG AGTGGGAGAT GGCTCGCTTC GGGGAGGCGG TGGTCGTTGG CAGGCCAGGC 
TCAGGCGATG GAGACTCGGA CCAGAGCAGG AACCGACAAG GAACCCCCGT CCCGGCCTCG 
GGGCCGGCGG CCGCCTACAA GCAGTCAAAA GCGCAGAGGG CGCGGACTAT GGCTTTGTAC 
AACCCCATAC CAGTCCGGCA GAACTGTTTC ACGGTCAACA GATCCCTGTT CATCTTCGGA 
GAAGATAACA TTGTCAGGAA ATACGCCAAG AAGCTCATCG ATTGGCCGCC ATTTGAGTAC 
ATGATCCTGG CCACCATCAT TGCCAACTGC ATCGTCTTGG CCCTGGAGCA GCATCTTCCT 
GAGGATGACA AGACCCCAAT GTCTCGAAGA CTGGAGAAGA CAGAACCATA TTTCATTGGG 
ATCTTCTGCT TTGAAGCTGG GATCAAAATT GTGGCTCTAG GGTTCATCTT CCATAAGGGT 
TCATACCTCC GTAATGGCTG GAATGTCATG GACTTCATCG TGGTCCTTAG TGGCATCCTG 
GCCACTGCGG GAACCCACTT CAACACCCAC GTGGACCTGC GGACCCTCCG GGCTGTGCGT 
GTCCTGAGGC CTTTAAAGCT GGTATCAGGA ATACCTAGCC TGCAGATCGT GCTGAAGTCT 
ATCATGAAGG CCATGGTGCC TCTTCTGCAG ATTGGCCTTC TGCTCTTCTT TGCCATCCTC 
ATGTTTGCTA TCATTGGCTT GGAGTTCTAC AGTGGCAAGT TGCATCGAGC ATGCTTCATG 
AACAATTCAG GTATTCTAGA GGGGTTTGAT CCTCCTCACC CGTGTGGTGT GCAAGGCTGC 
CCGGCTGGTT ATGAATGTAA GGACTGGATC GGCCCCAATG ACGGGATCAC CCAGTTTGAC 
AACATCCTTT TTGCCGTGCT GACTGTCTTC CAATGCATCA CTATGGAAGG GTGGACCACT 
GTGCTGTACA ATACCAATGA TGCCTTAGGA GCCACCTGGA ACTGGCTGTA CTTCATCCCT 
CTCATCATCA TCGGATCCTT CTTTGTTCTC AACCTCGTCC TGGGAGTGCT TTCCGGGGAA 
TTTGCCAAAG AAAGAGAGAG AGTGGAGAAC CGAAGAGCGT TCATGAAGCT CCGGCGCCAG 
CAACAGATTG AGCGAGAATT GAACGGCTAC CGTGCCTGGA TAGACAAAGC AGAGGAAGTC 
ATGCTTGCTG AAGAAAATAA AAACTCGGGA ACATCAGCCT TGGAAGTGCT TCGAAGGGCA 
ACCATCAAAA GGAGCCGGAC GGAGGCCATG ACCCGAGACT CCAGCGATGA GCACTGCGTT 
GATATCTCTT CAGTGGGCAC ACCTCTTGCC AGAGCCAGTA TCAAGAGCAC AAAAGTAGAT 
GGGGCCTCCT ATTTCCGGCA CAAGGAACGG CTTCTGCGCA TCTCTATCCG CCACATGGTC 
AAGTCCCAAG TCTTTTACTG GATCGTCCTG AGTGTTGTGG CTCTTAATAC TGCCTGTGTG 
GCCATTGTTC ATCACAACCA GCCACAGTGG CTCACTCACC TCCTCTACTA TGCAGAGTTT 
TTGTTTCTGG GACTCTTCCT GTTGGAGATG TCCCTGAAGA TGTATGGCAT GGGGCCACGC 
CTCTATTTTC ACTCATCCTT CAACTGCTTT GATTTTGGGG TCACAGTGGG CAGCATCTTT 
GAGGTTGTCT GGGCAATCTT CAGACCTGGC ACATCTTTTG GAATCAGTGT CTTACGAGCT 
CTCCGGCTCC TAAGGATATT TAAAATAACC AAGTATTGGG CATCCCTCCG GAATCTGGTT 
GTCTCCTTGA TGAGCTCCAT GAAGTCTATC ATCAGCTTAC TCTTCCTTCT CTTCCTCTTC 
ATCGTTGTCT TTGCTCTCCT GGGAATGCAG CTCTTTGGAG GCAGGTTTAA CTTTAATGAT 
GGGACTCCTT CAGCTAATTT TGACACCTTC CCTGCAGCTA TCATGACAGT GTTCCAGATC 
CTGACAGGGG AAGACTGGAA TGAGGTGATG TACAATGGCA TTCGCTCCCA GGGTGGGGTC 
AGCTCAGGCA TGTGGTCTGC CATCTACTTC ATTGTGCTCA CCTTGTTTGG AAACTACACG 
CTGCTAAATG TATTCTTGGC CATCGCTGTG GACAATCTGG CCAATGCCCA GGAACTGACC 
AAGGATGAGC AGGAGGAAGA AGAAGCCTTC AACCAGAAAC ATGCACTGCA GAAGGCCAAG 
GAGGTCAGCC CGATGTCTGC TCCCAACATG CCTTCCATTG AGAGGGAGCG GAGGCGCCGG 
CACCACATGT CTGTGTGGGA ACAACGCACC AGCCAGCTGA GGAGGCACAT GCAAATGTCT 
AGCCAAGAAG CCCTCAACAA AGAGGAGGCC CCGCCAATGA ACCCCCTCAA TCCACTCAAC 
CCACTGAGTC CTCTCAACCC CCTCAATGCT CACCCCAGCC TTTATCGGAG GCCCAGGCCC 
ATTGAGGGCC TAGCCCTAGG TCTGGGTCTT GAAAAGTGTG AAGAAGAACG CATCAGTCGT 
GGGGGCTCCC TCAAGGGGGA CATAGGGGGC CTGACTAGCG CCCTGGACAA CCAGAGGAGC 
CCCTTGTCCT TGGGCAAACG GGAGCCACCG TGGCTGCCTA GATCCTGTCA TGGAAACTGT 
GACCCAATCC AGCAGGAGGC TGGGGGAGGA GAGACTGTGG TGACTTTTGA GGACCGGGCC 
AGGCACAGGC AGAGTCAGAG GCGAAGCCGG CATCGCCGAG TCAGGACTGA GGGCAAGGAC 
TCTGCCTCTG CATCCCGAAG CAGGTCTGCC AGCCAGGAGC GGAGTCTGGA TGAAGGGGTG 
TCCGTTGAGG GGGAGAAGGA ACATGAGCCT CACAGCAGCC ACAGGAGCAA AGAGCCGACC 
ATCCACGAAG AGGAGAGAAC CCAGGACTTA AGGAGGACCA ACAGTCTGAT GGTCCCCAGG 
GGCTCTGGAC TGGTAGGGGC TCTTGATGAA GCAGAAACAC CTCTAGTCCA GCCCCAACCT 
GAGCTGGAAG TGGGGAAGGA TGCAGCTCTG ACAGAGCAGG AGGCTGAAGG CAGCAGTGAA 
CAAGCCCTTC TTGGGGACGT ACAGCTGGAT GTGGGCCGGG GCATTAGCCA GAGTGAACCG 
GATCTCTCCT GCATGACAGC CAACATGGAC AAGGCTACCA CGGAGAGCAC CAGTGTCACG 
GTCGCCATCC CTGATGTGGA CCCCTTGGTG GACTCGACAG TGGTGAACAT TAGCAACAAG 
ACGGATGGAG AAGCCAGTCC CTTGAAGGAG GCAGAGACTA AAGAGGAAGA AGAAGAGGTA 
GAGAAGAAGA AGAAGCAGAA GAAGGAGAAG CGAGAGACTG GAAAAGCCAT GGTGCCCCAT 
AGCTCTATGT TCATCTTCAG CACCACTAAC CCGATCCGCA GGGCCTGCCA CTACATTGTG 
AACCTACGCT ACTTTGAAAT GTGCATTCTC TTGGTGATTG CGGCCAGCAG CATTGCCCTG 
GCAGCCGAGG ACCCTGTACT AACTAACTCG GAGCGTAACA AAGTCCTGAG ATATTTCGAC 
TATGTTTTCA CAGGAGTGTT TACCTTTGAG ATGGTTATAA AGATGATAGA CCAAGGCTTG 
ATCCTGCAGG ATGGATCATA CTTCAGAGAC CTCTGGAACA TCCTGGACTT TGTAGTAGTG 
GTTGGTGCAT TGGTAGCATT TGCTCTGGCG AACGCTTTGG GAACCAACAA GGGGAGGGAT 
ATCAAGACTA TCAAGTCTCT GAGGGTACTT CGAGTTCTGC GGCCACTGAA AACCATCAAG 
CGCTTGCCCA AGCTGAAGGC TGTGTTTGAC TGCGTGGTGA CCTCCTTGAA GAATGTCTTC 
AACATACTCA TTGTCTACAA GCTCTTCATG TTCATCTTTG CCGTCATCGC TGTTCAGCTA 
TTCAAAGGAA AGTTCTTTTA TTGCACTGAC AGTTCCAAGG ACACAGAGAA GGAGTGCATA 
GGCAACTATG TAGACCACGA GAAAAACAAG ATGGAGGTGA AGGGCCGGGA ATGGAAGCGT 
CATGAATTCC ACTATGACAA CATCATCTGG GCCCTGCTTA CCCTCTTCAC AGTCTCTACA 
GGGGAAGGAT GGCCTCAGGT TCTGCAGCAC TCTGTAGATG TGACAGAGGA GGACAGAGGC 
CCAAGTCGCA GCAACCGCAT GGAGATGTCC ATCTTTTATG TGGTCTACTT TGTGGTCTTC 
CCTTTCTTCT TTGTCAATAT CTTCGTGGCT CTCATTATCA TCACCTTCCA GGAGCAAGGG 
GACAAGATGA TGGAGGAGTG TAGCCTGGAG AAGAATGAGC GGGCATGCAT CGACTTCGCC 
ATTAGTGCCA AACCTCTCAC CCGCTACATG CCACAGAACA GGCACACCTT CCAGTACCGT 
GTGTGGCACT TTGTAGTCTC CCCATCCTTT GAGTATACCA TCATGGCCAT GATTGCCTTG 
AACACTGTCG TGCTGATGAT GAAGTACTAC TCTGCTCCTT GCACCTATGA GCTCGCCTTA 
AAGTACCTGA ACATCGCCTT CACTATGGTG TTTTCCCTGG AGTGTGTCCT AAAGGTCATT 
GCTTTCGGCT TCCTGAACTA TTTCCGAGAT ACCTGGAACA TCTTTGACTT CATCACGGTG 
ATCGGCAGTA TCACAGAAAT TATCCTGACA GATAGCAAGC TGGTGAACAC CAGTGGCTTC 
AATATGAGCT TTCTGAAGCT CTTCCGAGCT GCACGGCTCA TAAAGCTTCT GAGACAGGGC 
TACACCATCC GCATCTTACT GTGGACCTTC GTGCAGTCCT TTAAGGCTCT TCCCTACGTC 
TGCCTTCTCA TTGCCATGCT TTTCTTCATC TACGCCATCA TTGGGATGCA GGTGTTTGGA 
AACATAAAGT TAGATGAGGA GAGTCACATC AACCGGCACA ACAACTTCCG GAGTTTCTTT 
GGATCCCTCA TGCTGCTTTT CAGGAGTGCC ACGGGGGAGG CTTGGCAGGA GATTATGCTG 
TCCTGTCTCG GTGAGAAGGG TTGTGAGCCT GACACCACTG CACCCTCAGG GCAGAATGAG 
AGTGAGCGCT GTGGCACTGA CCTGGCCTAC GTTTATTTTG TCTCCTTCAT CTTCTTCTGC 
TCCTTCCTGA TGCTCAACCT GTTCGTGGCT GTCATCATGG ACAACTTTGA GTACCTGACT 
CGAGATTCCT CCATCCTGGG GCCTCACCAC TTAGATGAGT TTGTCCGTGT CTGGGCAGAA 
TATGACAGAG CAGCATGCGG CAGGATCCCG TATAAGGATA TGTACAAATT AGTGCGGGTG 
ATCTCGCCTC CTCTGGGCCT GGGAGAGAAC TGCCCCTACA GGGTGGCTTG CAAGAGGCTG 
GTCCTGATGA ACATGCCAGT GGCTGAGGAC ATGACAGTCC ACTTCACCTC CACACTTATG 
GCTCTGATCC GGACAGCTCT GGACATTAAA ATCGCCAAAG GTGGTGCAGA CAGACAGCAG 
CTAGACTCGG AGCTGCAAAA AGAGACCCTG GCCATTTGGC CTCACCTGTC CCAAAAGATG 
TTGGATCTGC TTGTGCCCAT GCCCAAAGCC TCTGACCTGA CTGTGGGCAA GATCTATGCA 
GCAATGATGA TCATGGACTA CTATAAACAG AGTAAGGTAA AGAAACAGAG ACAGCAGCTG 
GAAGAACAGA AAAATGCACC CATGTTCCAG CGCATGGAGC CCTCATCACT GCCTCAGGAG 
ATCATTGCTA ATGCCAAAGC CCTGCCTTAT CTCCAGCAGG ACCCTGTTTC TGGCCTGAGT 
GGTCGGAGTG GATACCCTTC AATGAGTCCA CTCTCCCCTC AAGAAATATT CCAGTTGGCT 
TGTATGGACC CAGCGGATGA TGGACAATTC CAGGAACAAC AATCTCTGGT GGTAACAGAC 
CCTAGCTCTA TGCGACGTTC ATTTTCTACA ATTCGGGATA AGCGTTCAAA TTCCTCCTGG 
TTGGAGGAGT TCTCCATGGA ACGCAGCAGT GAAAACACTT ACAAGTCGCG GCGCAGAAGT 
TACCATTCCT CCCTGAGGCT GTCAGCACAC CGCCTGAATT CTGACTCTGG TCACAAGTCT 
GACACTCACC GATCAGGAGG CAGGGAGAGA GGCCGATCCA AAGAACGAAA GCACCTCCTC 
TCTCCAGATG TCTCCCGCTG CAATTCTGAA GAGCGAGGGA CCCAGGCCGA CTGGGAGTCC 
CCAGAACGTC GCCAGTCCAG GTCACCCAGT GAAGGAAGGT CACAGACCCC CAATAGACAG 
GGTACCGGTT CCCTAAGTGA GAGCTCCATC CCCTCTATTT CCGACACCAG CACCCCAAGA 
CGAAGCCGTC GCCAGCTCCC ACCTGTGCCA CCAAAGCCTC GTCCCCTCCT CTCCTACAGC 
TCCCTGATGA GACACACTGG CGGCATCTCT CCACCTCCTG ATGGAAGTGA GGGTGGATCC 
CCACTGGCCT CTCAAGCTCT GGAGAGCAAC AGCGCTTGCC TGACCGAGTC TTCCAACTCC 
TTGCACCCCC AGCAGGGCCA GCATCCTTCC CCACAGCACT ACATCTCCGA GCCCTATCTG 
GCCCTCCATG AAGACTCCCA CGCCTCAGAC TGTGGCGAGG AGGAGACACT CACCTTTGAG 
GCAGCCGTAG CCACTAGCTT GGGCCGGTCC AATACCATTG GCTCTGCGCC ACCCCTACGG 
CACAGCTGGC AGATGCCTAA TGGGCACTAT CGGCGGCGGA GGCGTGGGGG GCCTGGGCCT 
GGCATGATGT GTGGAGCTGT CAGTGACCTC CTGAGTGACA CGGAAGAAGA CGATAAGTGC 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017168313.1
CDS	594..7796
Translation	MVPEMRGLSPGRRLPEPTLCPATGGSPEPGAAGRRRHVLALPDEEAWRPQQCALLEANASDEVGMLPASPRSAVAGFDNFFPVQEGEGPGWEGSTTLDAGSSPFLPVSPEVMKRRRGGLIEQRDIIKAHEAHKMQSTPQARRKEWEMARFGEAVVVGRPGSGDGDSDQSRNRQGTPVPASGPAAAYKQSKAQRARTMALYNPIPVRQNCFTVNRSLFIFGEDNIVRKYAKKLIDWPPFEYMILATIIANCIVLALEQHLPEDDKTPMSRRLEKTEPYFIGIFCFEAGIKIVALGFIFHKGSYLRNGWNVMDFIVVLSGILATAGTHFNTHVDLRTLRAVRVLRPLKLVSGIPSLQIVLKSIMKAMVPLLQIGLLLFFAILMFAIIGLEFYSGKLHRACFMNNSGILEGFDPPHPCGVQGCPAGYECKDWIGPNDGITQFDNILFAVLTVFQCITMEGWTTVLYNTNDALGATWNWLYFIPLIIIGSFFVLNLVLGVLSGEFAKERERVENRRAFMKLRRQQQIERELNGYRAWIDKAEEVMLAEENKNSGTSALEVLRRATIKRSRTEAMTRDSSDEHCVDISSVGTPLARASIKSTKVDGASYFRHKERLLRISIRHMVKSQVFYWIVLSVVALNTACVAIVHHNQPQWLTHLLYYAEFLFLGLFLLEMSLKMYGMGPRLYFHSSFNCFDFGVTVGSIFEVVWAIFRPGTSFGISVLRALRLLRIFKITKYWASLRNLVVSLMSSMKSIISLLFLLFLFIVVFALLGMQLFGGRFNFNDGTPSANFDTFPAAIMTVFQILTGEDWNEVMYNGIRSQGGVSSGMWSAIYFIVLTLFGNYTLLNVFLAIAVDNLANAQELTKDEQEEEEAFNQKHALQKAKEVSPMSAPNMPSIERERRRRHHMSVWEQRTSQLRRHMQMSSQEALNKEEAPPMNPLNPLNPLSPLNPLNAHPSLYRRPRPIEGLALGLGLEKCEEERISRGGSLKGDIGGLTSALDNQRSPLSLGKREPPWLPRSCHGNCDPIQQEAGGGETVVTFEDRARHRQSQRRSRHRRVRTEGKDSASASRSRSASQERSLDEGVSVEGEKEHEPHSSHRSKEPTIHEEERTQDLRRTNSLMVPRGSGLVGALDEAETPLVQPQPELEVGKDAALTEQEAEGSSEQALLGDVQLDVGRGISQSEPDLSCMTANMDKATTESTSVTVAIPDVDPLVDSTVVNISNKTDGEASPLKEAETKEEEEEVEKKKKQKKEKRETGKAMVPHSSMFIFSTTNPIRRACHYIVNLRYFEMCILLVIAASSIALAAEDPVLTNSERNKVLRYFDYVFTGVFTFEMVIKMIDQGLILQDGSYFRDLWNILDFVVVVGALVAFALANALGTNKGRDIKTIKSLRVLRVLRPLKTIKRLPKLKAVFDCVVTSLKNVFNILIVYKLFMFIFAVIAVQLFKGKFFYCTDSSKDTEKECIGNYVDHEKNKMEVKGREWKRHEFHYDNIIWALLTLFTVSTGEGWPQVLQHSVDVTEEDRGPSRSNRMEMSIFYVVYFVVFPFFFVNIFVALIIITFQEQGDKMMEECSLEKNERACIDFAISAKPLTRYMPQNRHTFQYRVWHFVVSPSFEYTIMAMIALNTVVLMMKYYSAPCTYELALKYLNIAFTMVFSLECVLKVIAFGFLNYFRDTWNIFDFITVIGSITEIILTDSKLVNTSGFNMSFLKLFRAARLIKLLRQGYTIRILLWTFVQSFKALPYVCLLIAMLFFIYAIIGMQVFGNIKLDEESHINRHNNFRSFFGSLMLLFRSATGEAWQEIMLSCLGEKGCEPDTTAPSGQNESERCGTDLAYVYFVSFIFFCSFLMLNLFVAVIMDNFEYLTRDSSILGPHHLDEFVRVWAEYDRAACGRIPYKDMYKLVRVISPPLGLGENCPYRVACKRLVLMNMPVAEDMTVHFTSTLMALIRTALDIKIAKGGADRQQLDSELQKETLAIWPHLSQKMLDLLVPMPKASDLTVGKIYAAMMIMDYYKQSKVKKQRQQLEEQKNAPMFQRMEPSSLPQEIIANAKALPYLQQDPVSGLSGRSGYPSMSPLSPQEIFQLACMDPADDGQFQEQQSLVVTDPSSMRRSFSTIRDKRSNSSWLEEFSMERSSENTYKSRRRSYHSSLRLSAHRLNSDSGHKSDTHRSGGRERGRSKERKHLLSPDVSRCNSEERGTQADWESPERRQSRSPSEGRSQTPNRQGTGSLSESSIPSISDTSTPRRSRRQLPPVPPKPRPLLSYSSLMRHTGGISPPPDGSEGGSPLASQALESNSACLTESSNSLHPQQGQHPSPQHYISEPYLALHEDSHASDCGEEETLTFEAAVATSLGRSNTIGSAPPLRHSWQMPNGHYRRRRRGGPGPGMMCGAVSDLLSDTEEDDKC

Target ORF information:

RefSeq Version	XM_017312824.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus calcium channel, voltage-dependent, R type, alpha 1E subunit (Cacna1e), transcript variant X16, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017312824.1

ORF Insert Sequence:

ATGGTGCCCG AGATGCGTGG ACTGAGCCCA GGCCGAAGGC TGCCCGAGCC CACCCTGTGC 
CCGGCTACAG GAGGGAGCCC TGAGCCCGGG GCTGCTGGCA GGCGCCGCCA CGTCCTGGCT 
CTGCCTGATG AGGAGGCATG GCGGCCGCAG CAGTGCGCCC TGCTCGAGGC CAATGCCTCC 
GACGAGGTGG GCATGTTGCC TGCTTCCCCG CGCTCCGCGG TGGCCGGCTT CGACAACTTC 
TTCCCGGTGC AGGAAGGCGA GGGCCCGGGC TGGGAGGGCA GCACCACGCT GGATGCAGGC 
TCCAGCCCCT TCCTTCCTGT GAGCCCAGAG GTCATGAAGC GGAGGCGAGG GGGGCTCATC 
GAGCAGCGGG ACATCATCAA GGCCCACGAG GCACACAAGA TGCAGAGCAC CCCGCAGGCC 
CGGCGCAAGG AGTGGGAGAT GGCTCGCTTC GGGGAGGCGG TGGTCGTTGG CAGGCCAGGC 
TCAGGCGATG GAGACTCGGA CCAGAGCAGG AACCGACAAG GAACCCCCGT CCCGGCCTCG 
GGGCCGGCGG CCGCCTACAA GCAGTCAAAA GCGCAGAGGG CGCGGACTAT GGCTTTGTAC 
AACCCCATAC CAGTCCGGCA GAACTGTTTC ACGGTCAACA GATCCCTGTT CATCTTCGGA 
GAAGATAACA TTGTCAGGAA ATACGCCAAG AAGCTCATCG ATTGGCCGCC ATTTGAGTAC 
ATGATCCTGG CCACCATCAT TGCCAACTGC ATCGTCTTGG CCCTGGAGCA GCATCTTCCT 
GAGGATGACA AGACCCCAAT GTCTCGAAGA CTGGAGAAGA CAGAACCATA TTTCATTGGG 
ATCTTCTGCT TTGAAGCTGG GATCAAAATT GTGGCTCTAG GGTTCATCTT CCATAAGGGT 
TCATACCTCC GTAATGGCTG GAATGTCATG GACTTCATCG TGGTCCTTAG TGGCATCCTG 
GCCACTGCGG GAACCCACTT CAACACCCAC GTGGACCTGC GGACCCTCCG GGCTGTGCGT 
GTCCTGAGGC CTTTAAAGCT GGTATCAGGA ATACCTAGCC TGCAGATCGT GCTGAAGTCT 
ATCATGAAGG CCATGGTGCC TCTTCTGCAG ATTGGCCTTC TGCTCTTCTT TGCCATCCTC 
ATGTTTGCTA TCATTGGCTT GGAGTTCTAC AGTGGCAAGT TGCATCGAGC ATGCTTCATG 
AACAATTCAG GTATTCTAGA GGGGTTTGAT CCTCCTCACC CGTGTGGTGT GCAAGGCTGC 
CCGGCTGGTT ATGAATGTAA GGACTGGATC GGCCCCAATG ACGGGATCAC CCAGTTTGAC 
AACATCCTTT TTGCCGTGCT GACTGTCTTC CAATGCATCA CTATGGAAGG GTGGACCACT 
GTGCTGTACA ATACCAATGA TGCCTTAGGA GCCACCTGGA ACTGGCTGTA CTTCATCCCT 
CTCATCATCA TCGGATCCTT CTTTGTTCTC AACCTCGTCC TGGGAGTGCT TTCCGGGGAA 
TTTGCCAAAG AAAGAGAGAG AGTGGAGAAC CGAAGAGCGT TCATGAAGCT CCGGCGCCAG 
CAACAGATTG AGCGAGAATT GAACGGCTAC CGTGCCTGGA TAGACAAAGC AGAGGAAGTC 
ATGCTTGCTG AAGAAAATAA AAACTCGGGA ACATCAGCCT TGGAAGTGCT TCGAAGGGCA 
ACCATCAAAA GGAGCCGGAC GGAGGCCATG ACCCGAGACT CCAGCGATGA GCACTGCGTT 
GATATCTCTT CAGTGGGCAC ACCTCTTGCC AGAGCCAGTA TCAAGAGCAC AAAAGTAGAT 
GGGGCCTCCT ATTTCCGGCA CAAGGAACGG CTTCTGCGCA TCTCTATCCG CCACATGGTC 
AAGTCCCAAG TCTTTTACTG GATCGTCCTG AGTGTTGTGG CTCTTAATAC TGCCTGTGTG 
GCCATTGTTC ATCACAACCA GCCACAGTGG CTCACTCACC TCCTCTACTA TGCAGAGTTT 
TTGTTTCTGG GACTCTTCCT GTTGGAGATG TCCCTGAAGA TGTATGGCAT GGGGCCACGC 
CTCTATTTTC ACTCATCCTT CAACTGCTTT GATTTTGGGG TCACAGTGGG CAGCATCTTT 
GAGGTTGTCT GGGCAATCTT CAGACCTGGC ACATCTTTTG GAATCAGTGT CTTACGAGCT 
CTCCGGCTCC TAAGGATATT TAAAATAACC AAGTATTGGG CATCCCTCCG GAATCTGGTT 
GTCTCCTTGA TGAGCTCCAT GAAGTCTATC ATCAGCTTAC TCTTCCTTCT CTTCCTCTTC 
ATCGTTGTCT TTGCTCTCCT GGGAATGCAG CTCTTTGGAG GCAGGTTTAA CTTTAATGAT 
GGGACTCCTT CAGCTAATTT TGACACCTTC CCTGCAGCTA TCATGACAGT GTTCCAGATC 
CTGACAGGGG AAGACTGGAA TGAGGTGATG TACAATGGCA TTCGCTCCCA GGGTGGGGTC 
AGCTCAGGCA TGTGGTCTGC CATCTACTTC ATTGTGCTCA CCTTGTTTGG AAACTACACG 
CTGCTAAATG TATTCTTGGC CATCGCTGTG GACAATCTGG CCAATGCCCA GGAACTGACC 
AAGGATGAGC AGGAGGAAGA AGAAGCCTTC AACCAGAAAC ATGCACTGCA GAAGGCCAAG 
GAGGTCAGCC CGATGTCTGC TCCCAACATG CCTTCCATTG AGAGGGAGCG GAGGCGCCGG 
CACCACATGT CTGTGTGGGA ACAACGCACC AGCCAGCTGA GGAGGCACAT GCAAATGTCT 
AGCCAAGAAG CCCTCAACAA AGAGGAGGCC CCGCCAATGA ACCCCCTCAA TCCACTCAAC 
CCACTGAGTC CTCTCAACCC CCTCAATGCT CACCCCAGCC TTTATCGGAG GCCCAGGCCC 
ATTGAGGGCC TAGCCCTAGG TCTGGGTCTT GAAAAGTGTG AAGAAGAACG CATCAGTCGT 
GGGGGCTCCC TCAAGGGGGA CATAGGGGGC CTGACTAGCG CCCTGGACAA CCAGAGGAGC 
CCCTTGTCCT TGGGCAAACG GGAGCCACCG TGGCTGCCTA GATCCTGTCA TGGAAACTGT 
GACCCAATCC AGCAGGAGGC TGGGGGAGGA GAGACTGTGG TGACTTTTGA GGACCGGGCC 
AGGCACAGGC AGAGTCAGAG GCGAAGCCGG CATCGCCGAG TCAGGACTGA GGGCAAGGAC 
TCTGCCTCTG CATCCCGAAG CAGGTCTGCC AGCCAGGAGC GGAGTCTGGA TGAAGGGGTG 
TCCGTTGAGG GGGAGAAGGA ACATGAGCCT CACAGCAGCC ACAGGAGCAA AGAGCCGACC 
ATCCACGAAG AGGAGAGAAC CCAGGACTTA AGGAGGACCA ACAGTCTGAT GGTCCCCAGG 
GGCTCTGGAC TGGTAGGGGC TCTTGATGAA GCAGAAACAC CTCTAGTCCA GCCCCAACCT 
GAGCTGGAAG TGGGGAAGGA TGCAGCTCTG ACAGAGCAGG AGGCTGAAGG CAGCAGTGAA 
CAAGCCCTTC TTGGGGACGT ACAGCTGGAT GTGGGCCGGG GCATTAGCCA GAGTGAACCG 
GATCTCTCCT GCATGACAGC CAACATGGAC AAGGCTACCA CGGAGAGCAC CAGTGTCACG 
GTCGCCATCC CTGATGTGGA CCCCTTGGTG GACTCGACAG TGGTGAACAT TAGCAACAAG 
ACGGATGGAG AAGCCAGTCC CTTGAAGGAG GCAGAGACTA AAGAGGAAGA AGAAGAGGTA 
GAGAAGAAGA AGAAGCAGAA GAAGGAGAAG CGAGAGACTG GAAAAGCCAT GGTGCCCCAT 
AGCTCTATGT TCATCTTCAG CACCACTAAC CCGATCCGCA GGGCCTGCCA CTACATTGTG 
AACCTACGCT ACTTTGAAAT GTGCATTCTC TTGGTGATTG CGGCCAGCAG CATTGCCCTG 
GCAGCCGAGG ACCCTGTACT AACTAACTCG GAGCGTAACA AAGTCCTGAG ATATTTCGAC 
TATGTTTTCA CAGGAGTGTT TACCTTTGAG ATGGTTATAA AGATGATAGA CCAAGGCTTG 
ATCCTGCAGG ATGGATCATA CTTCAGAGAC CTCTGGAACA TCCTGGACTT TGTAGTAGTG 
GTTGGTGCAT TGGTAGCATT TGCTCTGGCG AACGCTTTGG GAACCAACAA GGGGAGGGAT 
ATCAAGACTA TCAAGTCTCT GAGGGTACTT CGAGTTCTGC GGCCACTGAA AACCATCAAG 
CGCTTGCCCA AGCTGAAGGC TGTGTTTGAC TGCGTGGTGA CCTCCTTGAA GAATGTCTTC 
AACATACTCA TTGTCTACAA GCTCTTCATG TTCATCTTTG CCGTCATCGC TGTTCAGCTA 
TTCAAAGGAA AGTTCTTTTA TTGCACTGAC AGTTCCAAGG ACACAGAGAA GGAGTGCATA 
GGCAACTATG TAGACCACGA GAAAAACAAG ATGGAGGTGA AGGGCCGGGA ATGGAAGCGT 
CATGAATTCC ACTATGACAA CATCATCTGG GCCCTGCTTA CCCTCTTCAC AGTCTCTACA 
GGGGAAGGAT GGCCTCAGGT TCTGCAGCAC TCTGTAGATG TGACAGAGGA GGACAGAGGC 
CCAAGTCGCA GCAACCGCAT GGAGATGTCC ATCTTTTATG TGGTCTACTT TGTGGTCTTC 
CCTTTCTTCT TTGTCAATAT CTTCGTGGCT CTCATTATCA TCACCTTCCA GGAGCAAGGG 
GACAAGATGA TGGAGGAGTG TAGCCTGGAG AAGAATGAGC GGGCATGCAT CGACTTCGCC 
ATTAGTGCCA AACCTCTCAC CCGCTACATG CCACAGAACA GGCACACCTT CCAGTACCGT 
GTGTGGCACT TTGTAGTCTC CCCATCCTTT GAGTATACCA TCATGGCCAT GATTGCCTTG 
AACACTGTCG TGCTGATGAT GAAGTACTAC TCTGCTCCTT GCACCTATGA GCTCGCCTTA 
AAGTACCTGA ACATCGCCTT CACTATGGTG TTTTCCCTGG AGTGTGTCCT AAAGGTCATT 
GCTTTCGGCT TCCTGAACTA TTTCCGAGAT ACCTGGAACA TCTTTGACTT CATCACGGTG 
ATCGGCAGTA TCACAGAAAT TATCCTGACA GATAGCAAGC TGGTGAACAC CAGTGGCTTC 
AATATGAGCT TTCTGAAGCT CTTCCGAGCT GCACGGCTCA TAAAGCTTCT GAGACAGGGC 
TACACCATCC GCATCTTACT GTGGACCTTC GTGCAGTCCT TTAAGGCTCT TCCCTACGTC 
TGCCTTCTCA TTGCCATGCT TTTCTTCATC TACGCCATCA TTGGGATGCA GGTGTTTGGA 
AACATAAAGT TAGATGAGGA GAGTCACATC AACCGGCACA ACAACTTCCG GAGTTTCTTT 
GGATCCCTCA TGCTGCTTTT CAGGAGTGCC ACGGGGGAGG CTTGGCAGGA GATTATGCTG 
TCCTGTCTCG GTGAGAAGGG TTGTGAGCCT GACACCACTG CACCCTCAGG GCAGAATGAG 
AGTGAGCGCT GTGGCACTGA CCTGGCCTAC GTTTATTTTG TCTCCTTCAT CTTCTTCTGC 
TCCTTCCTGA TGCTCAACCT GTTCGTGGCT GTCATCATGG ACAACTTTGA GTACCTGACT 
CGAGATTCCT CCATCCTGGG GCCTCACCAC TTAGATGAGT TTGTCCGTGT CTGGGCAGAA 
TATGACAGAG CAGCATGCGG CAGGATCCCG TATAAGGATA TGTACAAATT AGTGCGGGTG 
ATCTCGCCTC CTCTGGGCCT GGGAGAGAAC TGCCCCTACA GGGTGGCTTG CAAGAGGCTG 
GTCCTGATGA ACATGCCAGT GGCTGAGGAC ATGACAGTCC ACTTCACCTC CACACTTATG 
GCTCTGATCC GGACAGCTCT GGACATTAAA ATCGCCAAAG GTGGTGCAGA CAGACAGCAG 
CTAGACTCGG AGCTGCAAAA AGAGACCCTG GCCATTTGGC CTCACCTGTC CCAAAAGATG 
TTGGATCTGC TTGTGCCCAT GCCCAAAGCC TCTGACCTGA CTGTGGGCAA GATCTATGCA 
GCAATGATGA TCATGGACTA CTATAAACAG AGTAAGGTAA AGAAACAGAG ACAGCAGCTG 
GAAGAACAGA AAAATGCACC CATGTTCCAG CGCATGGAGC CCTCATCACT GCCTCAGGAG 
ATCATTGCTA ATGCCAAAGC CCTGCCTTAT CTCCAGCAGG ACCCTGTTTC TGGCCTGAGT 
GGTCGGAGTG GATACCCTTC AATGAGTCCA CTCTCCCCTC AAGAAATATT CCAGTTGGCT 
TGTATGGACC CAGCGGATGA TGGACAATTC CAGGAACAAC AATCTCTGGT GGTAACAGAC 
CCTAGCTCTA TGCGACGTTC ATTTTCTACA ATTCGGGATA AGCGTTCAAA TTCCTCCTGG 
TTGGAGGAGT TCTCCATGGA ACGCAGCAGT GAAAACACTT ACAAGTCGCG GCGCAGAAGT 
TACCATTCCT CCCTGAGGCT GTCAGCACAC CGCCTGAATT CTGACTCTGG TCACAAGTCT 
GACACTCACC GATCAGGAGG CAGGGAGAGA GGCCGATCCA AAGAACGAAA GCACCTCCTC 
TCTCCAGATG TCTCCCGCTG CAATTCTGAA GAGCGAGGGA CCCAGGCCGA CTGGGAGTCC 
CCAGAACGTC GCCAGTCCAG GTCACCCAGT GAAGGAAGGT CACAGACCCC CAATAGACAG 
GGTACCGGTT CCCTAAGTGA GAGCTCCATC CCCTCTATTT CCGACACCAG CACCCCAAGA 
CGAAGCCGTC GCCAGCTCCC ACCTGTGCCA CCAAAGCCTC GTCCCCTCCT CTCCTACAGC 
TCCCTGATGA GACACACTGG CGGCATCTCT CCACCTCCTG ATGGAAGTGA GGGTGGATCC 
CCACTGGCCT CTCAAGCTCT GGAGAGCAAC AGCGCTTGCC TGACCGAGTC TTCCAACTCC 
TTGCACCCCC AGCAGGGCCA GCATCCTTCC CCACAGCACT ACATCTCCGA GCCCTATCTG 
GCCCTCCATG AAGACTCCCA CGCCTCAGAC TGTGGCGAGG AGGAGACACT CACCTTTGAG 
GCAGCCGTAG CCACTAGCTT GGGCCGGTCC AATACCATTG GCTCTGCGCC ACCCCTACGG 
CACAGCTGGC AGATGCCTAA TGGGCACTAT CGGCGGCGGA GGCGTGGGGG GCCTGGGCCT 
GGCATGATGT GTGGAGCTGT CAGTGACCTC CTGAGTGACA CGGAAGAAGA CGATAAGTGC 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cacna1e ( XM_017312578.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017168067.1 Mus musculus calcium channel, voltage-dependent, R type, alpha 1E subunit (Cacna1e), transcript variant X1, mRNA.

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CloneID	OMu67260
Clone ID Related Accession (Same CDS sequence)	XM_017312578.1 , XM_030243926.1 , XM_017312594.1 , XM_030243929.1 , XM_011247909.2 , XM_017312578.2 , XM_011247909.3
Accession Version	XM_017312578.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7401bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	voltage-dependent R-type calcium channel subunit alpha-1E isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGCCCG AGATGCGTGG ACTGAGCCCA GGCCGAAGGC TGCCCGAGCC CACCCTGTGC 
CCGGCTACAG GAGGGAGCCC TGAGCCCGGG GCTGCTGGCA GGCGCCGCCA CGTCCTGGCT 
CTGCCTGATG AGGAGGCATG GCGGCCGCAG CAGTGCGCCC TGCTCGAGGC CAATGCCTCC 
GACGAGGTGG GCATGTTGCC TGCTTCCCCG CGCTCCGCGG TGGCCGGCTT CGACAACTTC 
TTCCCGGTGC AGGAAGGCGA GGGCCCGGGC TGGGAGGGCA GCACCACGCT GGATGCAGGC 
TCCAGCCCCT TCCTTCCTGT GAGCCCAGAG GTCATGAAGC GGAGGCGAGG GGGGCTCATC 
GAGCAGCGGG ACATCATCAA GGCCCACGAG GCACACAAGA TGCAGAGCAC CCCGCAGGCC 
CGGCGCAAGG AGTGGGAGAT GGCTCGCTTC GGGGAGGCGG TGGTCGTTGG CAGGCCAGGC 
TCAGGCGATG GAGACTCGGA CCAGAGCAGG AACCGACAAG GAACCCCCGT CCCGGCCTCG 
GGGCCGGCGG CCGCCTACAA GCAGTCAAAA GCGCAGAGGG CGCGGACTAT GGCTTTGTAC 
AACCCCATAC CAGTCCGGCA GAACTGTTTC ACGGTCAACA GATCCCTGTT CATCTTCGGA 
GAAGATAACA TTGTCAGGAA ATACGCCAAG AAGCTCATCG ATTGGCCGCC ATTTGAGTAC 
ATGATCCTGG CCACCATCAT TGCCAACTGC ATCGTCTTGG CCCTGGAGCA GCATCTTCCT 
GAGGATGACA AGACCCCAAT GTCTCGAAGA CTGGAGAAGA CAGAACCATA TTTCATTGGG 
ATCTTCTGCT TTGAAGCTGG GATCAAAATT GTGGCTCTAG GGTTCATCTT CCATAAGGGT 
TCATACCTCC GTAATGGCTG GAATGTCATG GACTTCATCG TGGTCCTTAG TGGCATCCTG 
GCCACTGCGG GAACCCACTT CAACACCCAC GTGGACCTGC GGACCCTCCG GGCTGTGCGT 
GTCCTGAGGC CTTTAAAGCT GGTATCAGGA ATACCTAGCC TGCAGATCGT GCTGAAGTCT 
ATCATGAAGG CCATGGTGCC TCTTCTGCAG ATTGGCCTTC TGCTCTTCTT TGCCATCCTC 
ATGTTTGCTA TCATTGGCTT GGAGTTCTAC AGTGGCAAGT TGCATCGAGC ATGCTTCATG 
AACAATTCAG GTATTCTAGA GGGGTTTGAT CCTCCTCACC CGTGTGGTGT GCAAGGCTGC 
CCGGCTGGTT ATGAATGTAA GGACTGGATC GGCCCCAATG ACGGGATCAC CCAGTTTGAC 
AACATCCTTT TTGCCGTGCT GACTGTCTTC CAATGCATCA CTATGGAAGG GTGGACCACT 
GTGCTGTACA ATACCAATGA TGCCTTAGGA GCCACCTGGA ACTGGCTGTA CTTCATCCCT 
CTCATCATCA TCGGATCCTT CTTTGTTCTC AACCTCGTCC TGGGAGTGCT TTCCGGGGAA 
TTTGCCAAAG AAAGAGAGAG AGTGGAGAAC CGAAGAGCGT TCATGAAGCT CCGGCGCCAG 
CAACAGATTG AGCGAGAATT GAACGGCTAC CGTGCCTGGA TAGACAAAGC AGAGGAAGTC 
ATGCTTGCTG AAGAAAATAA AAACTCGGGA ACATCAGCCT TGGAAGTGCT TCGAAGGGCA 
ACCATCAAAA GGAGCCGGAC GGAGGCCATG ACCCGAGACT CCAGCGATGA GCACTGCGTT 
GATATCTCTT CAGTGGGCAC ACCTCTTGCC AGAGCCAGTA TCAAGAGCAC AAAAGTAGAT 
GGGGCCTCCT ATTTCCGGCA CAAGGAACGG CTTCTGCGCA TCTCTATCCG CCACATGGTC 
AAGTCCCAAG TCTTTTACTG GATCGTCCTG AGTGTTGTGG CTCTTAATAC TGCCTGTGTG 
GCCATTGTTC ATCACAACCA GCCACAGTGG CTCACTCACC TCCTCTACTA TGCAGAGTTT 
TTGTTTCTGG GACTCTTCCT GTTGGAGATG TCCCTGAAGA TGTATGGCAT GGGGCCACGC 
CTCTATTTTC ACTCATCCTT CAACTGCTTT GATTTTGGGG TCACAGTGGG CAGCATCTTT 
GAGGTTGTCT GGGCAATCTT CAGACCTGGC ACATCTTTTG GAATCAGTGT CTTACGAGCT 
CTCCGGCTCC TAAGGATATT TAAAATAACC AAGTATTGGG CATCCCTCCG GAATCTGGTT 
GTCTCCTTGA TGAGCTCCAT GAAGTCTATC ATCAGCTTAC TCTTCCTTCT CTTCCTCTTC 
ATCGTTGTCT TTGCTCTCCT GGGAATGCAG CTCTTTGGAG GCAGGTTTAA CTTTAATGAT 
GGGACTCCTT CAGCTAATTT TGACACCTTC CCTGCAGCTA TCATGACAGT GTTCCAGATC 
CTGACAGGGG AAGACTGGAA TGAGGTGATG TACAATGGCA TTCGCTCCCA GGGTGGGGTC 
AGCTCAGGCA TGTGGTCTGC CATCTACTTC ATTGTGCTCA CCTTGTTTGG AAACTACACG 
CTGCTAAATG TATTCTTGGC CATCGCTGTG GACAATCTGG CCAATGCCCA GGAACTGACC 
AAGGATGAGC AGGAGGAAGA AGAAGCCTTC AACCAGAAAC ATGCACTGCA GAAGGCCAAG 
GAGGTCAGCC CGATGTCTGC TCCCAACATG CCTTCCATTG AAAGAGACAG AAGGAGAAGA 
CACCACATGT CGATGTGGGA GCCACGCAGC AGCCACCTGA GGGAGCGGAG GCGCCGGCAC 
CACATGTCTG TGTGGGAACA ACGCACCAGC CAGCTGAGGA GGCACATGCA AATGTCTAGC 
CAAGAAGCCC TCAACAAAGA GGAGGCCCCG CCAATGAACC CCCTCAATCC ACTCAACCCA 
CTGAGTCCTC TCAACCCCCT CAATGCTCAC CCCAGCCTTT ATCGGAGGCC CAGGCCCATT 
GAGGGCCTAG CCCTAGGTCT GGGTCTTGAA AAGTGTGAAG AAGAACGCAT CAGTCGTGGG 
GGCTCCCTCA AGGGGGACAT AGGGGGCCTG ACTAGCGCCC TGGACAACCA GAGGAGCCCC 
TTGTCCTTGG GCAAACGGGA GCCACCGTGG CTGCCTAGAT CCTGTCATGG AAACTGTGAC 
CCAATCCAGC AGGAGGCTGG GGGAGGAGAG ACTGTGGTGA CTTTTGAGGA CCGGGCCAGG 
CACAGGCAGA GTCAGAGGCG AAGCCGGCAT CGCCGAGTCA GGACTGAGGG CAAGGACTCT 
GCCTCTGCAT CCCGAAGCAG GTCTGCCAGC CAGGAGCGGA GTCTGGATGA AGGGGTGTCC 
GTTGAGGGGG AGAAGGAACA TGAGCCTCAC AGCAGCCACA GGAGCAAAGA GCCGACCATC 
CACGAAGAGG AGAGAACCCA GGACTTAAGG AGGACCAACA GTCTGATGGT CCCCAGGGGC 
TCTGGACTGG TAGGGGCTCT TGATGAAGCA GAAACACCTC TAGTCCAGCC CCAACCTGAG 
CTGGAAGTGG GGAAGGATGC AGCTCTGACA GAGCAGGAGG CTGAAGGCAG CAGTGAACAA 
GCCCTTCTTG GGGACGTACA GCTGGATGTG GGCCGGGGCA TTAGCCAGAG TGAACCGGAT 
CTCTCCTGCA TGACAGCCAA CATGGACAAG GCTACCACGG AGAGCACCAG TGTCACGGTC 
GCCATCCCTG ATGTGGACCC CTTGGTGGAC TCGACAGTGG TGAACATTAG CAACAAGACG 
GATGGAGAAG CCAGTCCCTT GAAGGAGGCA GAGACTAAAG AGGAAGAAGA AGAGGTAGAG 
AAGAAGAAGA AGCAGAAGAA GGAGAAGCGA GAGACTGGAA AAGCCATGGT GCCCCATAGC 
TCTATGTTCA TCTTCAGCAC CACTAACCCG ATCCGCAGGG CCTGCCACTA CATTGTGAAC 
CTACGCTACT TTGAAATGTG CATTCTCTTG GTGATTGCGG CCAGCAGCAT TGCCCTGGCA 
GCCGAGGACC CTGTACTAAC TAACTCGGAG CGTAACAAAG TCCTGAGATA TTTCGACTAT 
GTTTTCACAG GAGTGTTTAC CTTTGAGATG GTTATAAAGA TGATAGACCA AGGCTTGATC 
CTGCAGGATG GATCATACTT CAGAGACCTC TGGAACATCC TGGACTTTGT AGTAGTGGTT 
GGTGCATTGG TAGCATTTGC TCTGGCGAAC GCTTTGGGAA CCAACAAGGG GAGGGATATC 
AAGACTATCA AGTCTCTGAG GGTACTTCGA GTTCTGCGGC CACTGAAAAC CATCAAGCGC 
TTGCCCAAGC TGAAGGCTGT GTTTGACTGC GTGGTGACCT CCTTGAAGAA TGTCTTCAAC 
ATACTCATTG TCTACAAGCT CTTCATGTTC ATCTTTGCCG TCATCGCTGT TCAGCTATTC 
AAAGGAAAGT TCTTTTATTG CACTGACAGT TCCAAGGACA CAGAGAAGGA GTGCATAGGC 
AACTATGTAG ACCACGAGAA AAACAAGATG GAGGTGAAGG GCCGGGAATG GAAGCGTCAT 
GAATTCCACT ATGACAACAT CATCTGGGCC CTGCTTACCC TCTTCACAGT CTCTACAGGG 
GAAGGATGGC CTCAGGTTCT GCAGCACTCT GTAGATGTGA CAGAGGAGGA CAGAGGCCCA 
AGTCGCAGCA ACCGCATGGA GATGTCCATC TTTTATGTGG TCTACTTTGT GGTCTTCCCT 
TTCTTCTTTG TCAATATCTT CGTGGCTCTC ATTATCATCA CCTTCCAGGA GCAAGGGGAC 
AAGATGATGG AGGAGTGTAG CCTGGAGAAG AATGAGCGGG CATGCATCGA CTTCGCCATT 
AGTGCCAAAC CTCTCACCCG CTACATGCCA CAGAACAGGC ACACCTTCCA GTACCGTGTG 
TGGCACTTTG TAGTCTCCCC ATCCTTTGAG TATACCATCA TGGCCATGAT TGCCTTGAAC 
ACTGTCGTGC TGATGATGAA GTACTACTCT GCTCCTTGCA CCTATGAGCT CGCCTTAAAG 
TACCTGAACA TCGCCTTCAC TATGGTGTTT TCCCTGGAGT GTGTCCTAAA GGTCATTGCT 
TTCGGCTTCC TGAACTATTT CCGAGATACC TGGAACATCT TTGACTTCAT CACGGTGATC 
GGCAGTATCA CAGAAATTAT CCTGACAGAT AGCAAGCTGG TGAACACCAG TGGCTTCAAT 
ATGAGCTTTC TGAAGCTCTT CCGAGCTGCA CGGCTCATAA AGCTTCTGAG ACAGGGCTAC 
ACCATCCGCA TCTTACTGTG GACCTTCGTG CAGTCCTTTA AGGCTCTTCC CTACGTCTGC 
CTTCTCATTG CCATGCTTTT CTTCATCTAC GCCATCATTG GGATGCAGGT GTTTGGAAAC 
ATAAAGTTAG ATGAGGAGAG TCACATCAAC CGGCACAACA ACTTCCGGAG TTTCTTTGGA 
TCCCTCATGC TGCTTTTCAG GAGTGCCACG GGGGAGGCTT GGCAGGAGAT TATGCTGTCC 
TGTCTCGGTG AGAAGGGTTG TGAGCCTGAC ACCACTGCAC CCTCAGGGCA GAATGAGAGT 
GAGCGCTGTG GCACTGACCT GGCCTACGTT TATTTTGTCT CCTTCATCTT CTTCTGCTCC 
TTCCTGATGC TCAACCTGTT CGTGGCTGTC ATCATGGACA ACTTTGAGTA CCTGACTCGA 
GATTCCTCCA TCCTGGGGCC TCACCACTTA GATGAGTTTG TCCGTGTCTG GGCAGAATAT 
GACAGAGCAG CATGTGGCCG CATCCATTAC ACTGAGATGT ATGAAATGCT GACTCTCATG 
TCACCACCGC TAGGCCTCGG CAAGAGATGT CCCTCCAAAG TGGCCTATAA GAGGCTGGTC 
CTGATGAACA TGCCAGTGGC TGAGGACATG ACAGTCCACT TCACCTCCAC ACTTATGGCT 
CTGATCCGGA CAGCTCTGGA CATTAAAATC GCCAAAGGTG GTGCAGACAG ACAGCAGCTA 
GACTCGGAGC TGCAAAAAGA GACCCTGGCC ATTTGGCCTC ACCTGTCCCA AAAGATGTTG 
GATCTGCTTG TGCCCATGCC CAAAGCCTCT GACCTGACTG TGGGCAAGAT CTATGCAGCA 
ATGATGATCA TGGACTACTA TAAACAGAGT AAGGTAAAGA AACAGAGACA GCAGCTGGAA 
GAACAGAAAA ATGCACCCAT GTTCCAGCGC ATGGAGCCCT CATCACTGCC TCAGGAGATC 
ATTGCTAATG CCAAAGCCCT GCCTTATCTC CAGCAGGACC CTGTTTCTGG CCTGAGTGGT 
CGGAGTGGAT ACCCTTCAAT GAGTCCACTC TCCCCTCAAG AAATATTCCA GTTGGCTTGT 
ATGGACCCAG CGGATGATGG ACAATTCCAG GAACAACAAT CTCTGGTGAA TGCACAAGAG 
CCAGAGGTTA GTGAATTAAA AAGTGTGCAG TCCTCTAACC ATGGCATCTA CCTTCCTCCG 
GACACCCAGG AGCATGCGGG ATCTGGGAGG GCATCCTCTA TGCCACGTCT GACTATGGAT 
CCCCAGGTGG TAACAGACCC TAGCTCTATG CGACGTTCAT TTTCTACAAT TCGGGATAAG 
CGTTCAAATT CCTCCTGGTT GGAGGAGTTC TCCATGGAAC GCAGCAGTGA AAACACTTAC 
AAGTCGCGGC GCAGAAGTTA CCATTCCTCC CTGAGGCTGT CAGCACACCG CCTGAATTCT 
GACTCTGGTC ACAAGTCTGA CACTCACCGA TCAGGAGGCA GGGAGAGAGG CCGATCCAAA 
GAACGAAAGC ACCTCCTCTC TCCAGATGTC TCCCGCTGCA ATTCTGAAGA GCGAGGGACC 
CAGGCCGACT GGGAGTCCCC AGAACGTCGC CAGTCCAGGT CACCCAGTGA AGGAAGGTCA 
CAGACCCCCA ATAGACAGGG TACCGGTTCC CTAAGTGAGA GCTCCATCCC CTCTATTTCC 
GACACCAGCA CCCCAAGACG AAGCCGTCGC CAGCTCCCAC CTGTGCCACC AAAGCCTCGT 
CCCCTCCTCT CCTACAGCTC CCTGATGAGA CACACTGGCG GCATCTCTCC ACCTCCTGAT 
GGAAGTGAGG GTGGATCCCC ACTGGCCTCT CAAGCTCTGG AGAGCAACAG CGCTTGCCTG 
ACCGAGTCTT CCAACTCCTT GCACCCCCAG CAGGGCCAGC ATCCTTCCCC ACAGCACTAC 
ATCTCCGAGC CCTATCTGGC CCTCCATGAA GACTCCCACG CCTCAGACTG TGGCGAGGAG 
GAGACACTCA CCTTTGAGGC AGCCGTAGCC ACTAGCTTGG GCCGGTCCAA TACCATTGGC 
TCTGCGCCAC CCCTACGGCA CAGCTGGCAG ATGCCTAATG GGCACTATCG GCGGCGGAGG 
CGTGGGGGGC CTGGGCCTGG CATGATGTGT GGAGCTGTCA GTGACCTCCT GAGTGACACG 
GAAGAAGACG ATAAGTGCTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017168067.1
CDS	597..7997
Translation	MVPEMRGLSPGRRLPEPTLCPATGGSPEPGAAGRRRHVLALPDEEAWRPQQCALLEANASDEVGMLPASPRSAVAGFDNFFPVQEGEGPGWEGSTTLDAGSSPFLPVSPEVMKRRRGGLIEQRDIIKAHEAHKMQSTPQARRKEWEMARFGEAVVVGRPGSGDGDSDQSRNRQGTPVPASGPAAAYKQSKAQRARTMALYNPIPVRQNCFTVNRSLFIFGEDNIVRKYAKKLIDWPPFEYMILATIIANCIVLALEQHLPEDDKTPMSRRLEKTEPYFIGIFCFEAGIKIVALGFIFHKGSYLRNGWNVMDFIVVLSGILATAGTHFNTHVDLRTLRAVRVLRPLKLVSGIPSLQIVLKSIMKAMVPLLQIGLLLFFAILMFAIIGLEFYSGKLHRACFMNNSGILEGFDPPHPCGVQGCPAGYECKDWIGPNDGITQFDNILFAVLTVFQCITMEGWTTVLYNTNDALGATWNWLYFIPLIIIGSFFVLNLVLGVLSGEFAKERERVENRRAFMKLRRQQQIERELNGYRAWIDKAEEVMLAEENKNSGTSALEVLRRATIKRSRTEAMTRDSSDEHCVDISSVGTPLARASIKSTKVDGASYFRHKERLLRISIRHMVKSQVFYWIVLSVVALNTACVAIVHHNQPQWLTHLLYYAEFLFLGLFLLEMSLKMYGMGPRLYFHSSFNCFDFGVTVGSIFEVVWAIFRPGTSFGISVLRALRLLRIFKITKYWASLRNLVVSLMSSMKSIISLLFLLFLFIVVFALLGMQLFGGRFNFNDGTPSANFDTFPAAIMTVFQILTGEDWNEVMYNGIRSQGGVSSGMWSAIYFIVLTLFGNYTLLNVFLAIAVDNLANAQELTKDEQEEEEAFNQKHALQKAKEVSPMSAPNMPSIERDRRRRHHMSMWEPRSSHLRERRRRHHMSVWEQRTSQLRRHMQMSSQEALNKEEAPPMNPLNPLNPLSPLNPLNAHPSLYRRPRPIEGLALGLGLEKCEEERISRGGSLKGDIGGLTSALDNQRSPLSLGKREPPWLPRSCHGNCDPIQQEAGGGETVVTFEDRARHRQSQRRSRHRRVRTEGKDSASASRSRSASQERSLDEGVSVEGEKEHEPHSSHRSKEPTIHEEERTQDLRRTNSLMVPRGSGLVGALDEAETPLVQPQPELEVGKDAALTEQEAEGSSEQALLGDVQLDVGRGISQSEPDLSCMTANMDKATTESTSVTVAIPDVDPLVDSTVVNISNKTDGEASPLKEAETKEEEEEVEKKKKQKKEKRETGKAMVPHSSMFIFSTTNPIRRACHYIVNLRYFEMCILLVIAASSIALAAEDPVLTNSERNKVLRYFDYVFTGVFTFEMVIKMIDQGLILQDGSYFRDLWNILDFVVVVGALVAFALANALGTNKGRDIKTIKSLRVLRVLRPLKTIKRLPKLKAVFDCVVTSLKNVFNILIVYKLFMFIFAVIAVQLFKGKFFYCTDSSKDTEKECIGNYVDHEKNKMEVKGREWKRHEFHYDNIIWALLTLFTVSTGEGWPQVLQHSVDVTEEDRGPSRSNRMEMSIFYVVYFVVFPFFFVNIFVALIIITFQEQGDKMMEECSLEKNERACIDFAISAKPLTRYMPQNRHTFQYRVWHFVVSPSFEYTIMAMIALNTVVLMMKYYSAPCTYELALKYLNIAFTMVFSLECVLKVIAFGFLNYFRDTWNIFDFITVIGSITEIILTDSKLVNTSGFNMSFLKLFRAARLIKLLRQGYTIRILLWTFVQSFKALPYVCLLIAMLFFIYAIIGMQVFGNIKLDEESHINRHNNFRSFFGSLMLLFRSATGEAWQEIMLSCLGEKGCEPDTTAPSGQNESERCGTDLAYVYFVSFIFFCSFLMLNLFVAVIMDNFEYLTRDSSILGPHHLDEFVRVWAEYDRAACGRIHYTEMYEMLTLMSPPLGLGKRCPSKVAYKRLVLMNMPVAEDMTVHFTSTLMALIRTALDIKIAKGGADRQQLDSELQKETLAIWPHLSQKMLDLLVPMPKASDLTVGKIYAAMMIMDYYKQSKVKKQRQQLEEQKNAPMFQRMEPSSLPQEIIANAKALPYLQQDPVSGLSGRSGYPSMSPLSPQEIFQLACMDPADDGQFQEQQSLVNAQEPEVSELKSVQSSNHGIYLPPDTQEHAGSGRASSMPRLTMDPQVVTDPSSMRRSFSTIRDKRSNSSWLEEFSMERSSENTYKSRRRSYHSSLRLSAHRLNSDSGHKSDTHRSGGRERGRSKERKHLLSPDVSRCNSEERGTQADWESPERRQSRSPSEGRSQTPNRQGTGSLSESSIPSISDTSTPRRSRRQLPPVPPKPRPLLSYSSLMRHTGGISPPPDGSEGGSPLASQALESNSACLTESSNSLHPQQGQHPSPQHYISEPYLALHEDSHASDCGEEETLTFEAAVATSLGRSNTIGSAPPLRHSWQMPNGHYRRRRRGGPGPGMMCGAVSDLLSDTEEDDKC

Target ORF information:

RefSeq Version	XM_017312578.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus calcium channel, voltage-dependent, R type, alpha 1E subunit (Cacna1e), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017312578.1

ORF Insert Sequence:

ATGGTGCCCG AGATGCGTGG ACTGAGCCCA GGCCGAAGGC TGCCCGAGCC CACCCTGTGC 
CCGGCTACAG GAGGGAGCCC TGAGCCCGGG GCTGCTGGCA GGCGCCGCCA CGTCCTGGCT 
CTGCCTGATG AGGAGGCATG GCGGCCGCAG CAGTGCGCCC TGCTCGAGGC CAATGCCTCC 
GACGAGGTGG GCATGTTGCC TGCTTCCCCG CGCTCCGCGG TGGCCGGCTT CGACAACTTC 
TTCCCGGTGC AGGAAGGCGA GGGCCCGGGC TGGGAGGGCA GCACCACGCT GGATGCAGGC 
TCCAGCCCCT TCCTTCCTGT GAGCCCAGAG GTCATGAAGC GGAGGCGAGG GGGGCTCATC 
GAGCAGCGGG ACATCATCAA GGCCCACGAG GCACACAAGA TGCAGAGCAC CCCGCAGGCC 
CGGCGCAAGG AGTGGGAGAT GGCTCGCTTC GGGGAGGCGG TGGTCGTTGG CAGGCCAGGC 
TCAGGCGATG GAGACTCGGA CCAGAGCAGG AACCGACAAG GAACCCCCGT CCCGGCCTCG 
GGGCCGGCGG CCGCCTACAA GCAGTCAAAA GCGCAGAGGG CGCGGACTAT GGCTTTGTAC 
AACCCCATAC CAGTCCGGCA GAACTGTTTC ACGGTCAACA GATCCCTGTT CATCTTCGGA 
GAAGATAACA TTGTCAGGAA ATACGCCAAG AAGCTCATCG ATTGGCCGCC ATTTGAGTAC 
ATGATCCTGG CCACCATCAT TGCCAACTGC ATCGTCTTGG CCCTGGAGCA GCATCTTCCT 
GAGGATGACA AGACCCCAAT GTCTCGAAGA CTGGAGAAGA CAGAACCATA TTTCATTGGG 
ATCTTCTGCT TTGAAGCTGG GATCAAAATT GTGGCTCTAG GGTTCATCTT CCATAAGGGT 
TCATACCTCC GTAATGGCTG GAATGTCATG GACTTCATCG TGGTCCTTAG TGGCATCCTG 
GCCACTGCGG GAACCCACTT CAACACCCAC GTGGACCTGC GGACCCTCCG GGCTGTGCGT 
GTCCTGAGGC CTTTAAAGCT GGTATCAGGA ATACCTAGCC TGCAGATCGT GCTGAAGTCT 
ATCATGAAGG CCATGGTGCC TCTTCTGCAG ATTGGCCTTC TGCTCTTCTT TGCCATCCTC 
ATGTTTGCTA TCATTGGCTT GGAGTTCTAC AGTGGCAAGT TGCATCGAGC ATGCTTCATG 
AACAATTCAG GTATTCTAGA GGGGTTTGAT CCTCCTCACC CGTGTGGTGT GCAAGGCTGC 
CCGGCTGGTT ATGAATGTAA GGACTGGATC GGCCCCAATG ACGGGATCAC CCAGTTTGAC 
AACATCCTTT TTGCCGTGCT GACTGTCTTC CAATGCATCA CTATGGAAGG GTGGACCACT 
GTGCTGTACA ATACCAATGA TGCCTTAGGA GCCACCTGGA ACTGGCTGTA CTTCATCCCT 
CTCATCATCA TCGGATCCTT CTTTGTTCTC AACCTCGTCC TGGGAGTGCT TTCCGGGGAA 
TTTGCCAAAG AAAGAGAGAG AGTGGAGAAC CGAAGAGCGT TCATGAAGCT CCGGCGCCAG 
CAACAGATTG AGCGAGAATT GAACGGCTAC CGTGCCTGGA TAGACAAAGC AGAGGAAGTC 
ATGCTTGCTG AAGAAAATAA AAACTCGGGA ACATCAGCCT TGGAAGTGCT TCGAAGGGCA 
ACCATCAAAA GGAGCCGGAC GGAGGCCATG ACCCGAGACT CCAGCGATGA GCACTGCGTT 
GATATCTCTT CAGTGGGCAC ACCTCTTGCC AGAGCCAGTA TCAAGAGCAC AAAAGTAGAT 
GGGGCCTCCT ATTTCCGGCA CAAGGAACGG CTTCTGCGCA TCTCTATCCG CCACATGGTC 
AAGTCCCAAG TCTTTTACTG GATCGTCCTG AGTGTTGTGG CTCTTAATAC TGCCTGTGTG 
GCCATTGTTC ATCACAACCA GCCACAGTGG CTCACTCACC TCCTCTACTA TGCAGAGTTT 
TTGTTTCTGG GACTCTTCCT GTTGGAGATG TCCCTGAAGA TGTATGGCAT GGGGCCACGC 
CTCTATTTTC ACTCATCCTT CAACTGCTTT GATTTTGGGG TCACAGTGGG CAGCATCTTT 
GAGGTTGTCT GGGCAATCTT CAGACCTGGC ACATCTTTTG GAATCAGTGT CTTACGAGCT 
CTCCGGCTCC TAAGGATATT TAAAATAACC AAGTATTGGG CATCCCTCCG GAATCTGGTT 
GTCTCCTTGA TGAGCTCCAT GAAGTCTATC ATCAGCTTAC TCTTCCTTCT CTTCCTCTTC 
ATCGTTGTCT TTGCTCTCCT GGGAATGCAG CTCTTTGGAG GCAGGTTTAA CTTTAATGAT 
GGGACTCCTT CAGCTAATTT TGACACCTTC CCTGCAGCTA TCATGACAGT GTTCCAGATC 
CTGACAGGGG AAGACTGGAA TGAGGTGATG TACAATGGCA TTCGCTCCCA GGGTGGGGTC 
AGCTCAGGCA TGTGGTCTGC CATCTACTTC ATTGTGCTCA CCTTGTTTGG AAACTACACG 
CTGCTAAATG TATTCTTGGC CATCGCTGTG GACAATCTGG CCAATGCCCA GGAACTGACC 
AAGGATGAGC AGGAGGAAGA AGAAGCCTTC AACCAGAAAC ATGCACTGCA GAAGGCCAAG 
GAGGTCAGCC CGATGTCTGC TCCCAACATG CCTTCCATTG AAAGAGACAG AAGGAGAAGA 
CACCACATGT CGATGTGGGA GCCACGCAGC AGCCACCTGA GGGAGCGGAG GCGCCGGCAC 
CACATGTCTG TGTGGGAACA ACGCACCAGC CAGCTGAGGA GGCACATGCA AATGTCTAGC 
CAAGAAGCCC TCAACAAAGA GGAGGCCCCG CCAATGAACC CCCTCAATCC ACTCAACCCA 
CTGAGTCCTC TCAACCCCCT CAATGCTCAC CCCAGCCTTT ATCGGAGGCC CAGGCCCATT 
GAGGGCCTAG CCCTAGGTCT GGGTCTTGAA AAGTGTGAAG AAGAACGCAT CAGTCGTGGG 
GGCTCCCTCA AGGGGGACAT AGGGGGCCTG ACTAGCGCCC TGGACAACCA GAGGAGCCCC 
TTGTCCTTGG GCAAACGGGA GCCACCGTGG CTGCCTAGAT CCTGTCATGG AAACTGTGAC 
CCAATCCAGC AGGAGGCTGG GGGAGGAGAG ACTGTGGTGA CTTTTGAGGA CCGGGCCAGG 
CACAGGCAGA GTCAGAGGCG AAGCCGGCAT CGCCGAGTCA GGACTGAGGG CAAGGACTCT 
GCCTCTGCAT CCCGAAGCAG GTCTGCCAGC CAGGAGCGGA GTCTGGATGA AGGGGTGTCC 
GTTGAGGGGG AGAAGGAACA TGAGCCTCAC AGCAGCCACA GGAGCAAAGA GCCGACCATC 
CACGAAGAGG AGAGAACCCA GGACTTAAGG AGGACCAACA GTCTGATGGT CCCCAGGGGC 
TCTGGACTGG TAGGGGCTCT TGATGAAGCA GAAACACCTC TAGTCCAGCC CCAACCTGAG 
CTGGAAGTGG GGAAGGATGC AGCTCTGACA GAGCAGGAGG CTGAAGGCAG CAGTGAACAA 
GCCCTTCTTG GGGACGTACA GCTGGATGTG GGCCGGGGCA TTAGCCAGAG TGAACCGGAT 
CTCTCCTGCA TGACAGCCAA CATGGACAAG GCTACCACGG AGAGCACCAG TGTCACGGTC 
GCCATCCCTG ATGTGGACCC CTTGGTGGAC TCGACAGTGG TGAACATTAG CAACAAGACG 
GATGGAGAAG CCAGTCCCTT GAAGGAGGCA GAGACTAAAG AGGAAGAAGA AGAGGTAGAG 
AAGAAGAAGA AGCAGAAGAA GGAGAAGCGA GAGACTGGAA AAGCCATGGT GCCCCATAGC 
TCTATGTTCA TCTTCAGCAC CACTAACCCG ATCCGCAGGG CCTGCCACTA CATTGTGAAC 
CTACGCTACT TTGAAATGTG CATTCTCTTG GTGATTGCGG CCAGCAGCAT TGCCCTGGCA 
GCCGAGGACC CTGTACTAAC TAACTCGGAG CGTAACAAAG TCCTGAGATA TTTCGACTAT 
GTTTTCACAG GAGTGTTTAC CTTTGAGATG GTTATAAAGA TGATAGACCA AGGCTTGATC 
CTGCAGGATG GATCATACTT CAGAGACCTC TGGAACATCC TGGACTTTGT AGTAGTGGTT 
GGTGCATTGG TAGCATTTGC TCTGGCGAAC GCTTTGGGAA CCAACAAGGG GAGGGATATC 
AAGACTATCA AGTCTCTGAG GGTACTTCGA GTTCTGCGGC CACTGAAAAC CATCAAGCGC 
TTGCCCAAGC TGAAGGCTGT GTTTGACTGC GTGGTGACCT CCTTGAAGAA TGTCTTCAAC 
ATACTCATTG TCTACAAGCT CTTCATGTTC ATCTTTGCCG TCATCGCTGT TCAGCTATTC 
AAAGGAAAGT TCTTTTATTG CACTGACAGT TCCAAGGACA CAGAGAAGGA GTGCATAGGC 
AACTATGTAG ACCACGAGAA AAACAAGATG GAGGTGAAGG GCCGGGAATG GAAGCGTCAT 
GAATTCCACT ATGACAACAT CATCTGGGCC CTGCTTACCC TCTTCACAGT CTCTACAGGG 
GAAGGATGGC CTCAGGTTCT GCAGCACTCT GTAGATGTGA CAGAGGAGGA CAGAGGCCCA 
AGTCGCAGCA ACCGCATGGA GATGTCCATC TTTTATGTGG TCTACTTTGT GGTCTTCCCT 
TTCTTCTTTG TCAATATCTT CGTGGCTCTC ATTATCATCA CCTTCCAGGA GCAAGGGGAC 
AAGATGATGG AGGAGTGTAG CCTGGAGAAG AATGAGCGGG CATGCATCGA CTTCGCCATT 
AGTGCCAAAC CTCTCACCCG CTACATGCCA CAGAACAGGC ACACCTTCCA GTACCGTGTG 
TGGCACTTTG TAGTCTCCCC ATCCTTTGAG TATACCATCA TGGCCATGAT TGCCTTGAAC 
ACTGTCGTGC TGATGATGAA GTACTACTCT GCTCCTTGCA CCTATGAGCT CGCCTTAAAG 
TACCTGAACA TCGCCTTCAC TATGGTGTTT TCCCTGGAGT GTGTCCTAAA GGTCATTGCT 
TTCGGCTTCC TGAACTATTT CCGAGATACC TGGAACATCT TTGACTTCAT CACGGTGATC 
GGCAGTATCA CAGAAATTAT CCTGACAGAT AGCAAGCTGG TGAACACCAG TGGCTTCAAT 
ATGAGCTTTC TGAAGCTCTT CCGAGCTGCA CGGCTCATAA AGCTTCTGAG ACAGGGCTAC 
ACCATCCGCA TCTTACTGTG GACCTTCGTG CAGTCCTTTA AGGCTCTTCC CTACGTCTGC 
CTTCTCATTG CCATGCTTTT CTTCATCTAC GCCATCATTG GGATGCAGGT GTTTGGAAAC 
ATAAAGTTAG ATGAGGAGAG TCACATCAAC CGGCACAACA ACTTCCGGAG TTTCTTTGGA 
TCCCTCATGC TGCTTTTCAG GAGTGCCACG GGGGAGGCTT GGCAGGAGAT TATGCTGTCC 
TGTCTCGGTG AGAAGGGTTG TGAGCCTGAC ACCACTGCAC CCTCAGGGCA GAATGAGAGT 
GAGCGCTGTG GCACTGACCT GGCCTACGTT TATTTTGTCT CCTTCATCTT CTTCTGCTCC 
TTCCTGATGC TCAACCTGTT CGTGGCTGTC ATCATGGACA ACTTTGAGTA CCTGACTCGA 
GATTCCTCCA TCCTGGGGCC TCACCACTTA GATGAGTTTG TCCGTGTCTG GGCAGAATAT 
GACAGAGCAG CATGTGGCCG CATCCATTAC ACTGAGATGT ATGAAATGCT GACTCTCATG 
TCACCACCGC TAGGCCTCGG CAAGAGATGT CCCTCCAAAG TGGCCTATAA GAGGCTGGTC 
CTGATGAACA TGCCAGTGGC TGAGGACATG ACAGTCCACT TCACCTCCAC ACTTATGGCT 
CTGATCCGGA CAGCTCTGGA CATTAAAATC GCCAAAGGTG GTGCAGACAG ACAGCAGCTA 
GACTCGGAGC TGCAAAAAGA GACCCTGGCC ATTTGGCCTC ACCTGTCCCA AAAGATGTTG 
GATCTGCTTG TGCCCATGCC CAAAGCCTCT GACCTGACTG TGGGCAAGAT CTATGCAGCA 
ATGATGATCA TGGACTACTA TAAACAGAGT AAGGTAAAGA AACAGAGACA GCAGCTGGAA 
GAACAGAAAA ATGCACCCAT GTTCCAGCGC ATGGAGCCCT CATCACTGCC TCAGGAGATC 
ATTGCTAATG CCAAAGCCCT GCCTTATCTC CAGCAGGACC CTGTTTCTGG CCTGAGTGGT 
CGGAGTGGAT ACCCTTCAAT GAGTCCACTC TCCCCTCAAG AAATATTCCA GTTGGCTTGT 
ATGGACCCAG CGGATGATGG ACAATTCCAG GAACAACAAT CTCTGGTGAA TGCACAAGAG 
CCAGAGGTTA GTGAATTAAA AAGTGTGCAG TCCTCTAACC ATGGCATCTA CCTTCCTCCG 
GACACCCAGG AGCATGCGGG ATCTGGGAGG GCATCCTCTA TGCCACGTCT GACTATGGAT 
CCCCAGGTGG TAACAGACCC TAGCTCTATG CGACGTTCAT TTTCTACAAT TCGGGATAAG 
CGTTCAAATT CCTCCTGGTT GGAGGAGTTC TCCATGGAAC GCAGCAGTGA AAACACTTAC 
AAGTCGCGGC GCAGAAGTTA CCATTCCTCC CTGAGGCTGT CAGCACACCG CCTGAATTCT 
GACTCTGGTC ACAAGTCTGA CACTCACCGA TCAGGAGGCA GGGAGAGAGG CCGATCCAAA 
GAACGAAAGC ACCTCCTCTC TCCAGATGTC TCCCGCTGCA ATTCTGAAGA GCGAGGGACC 
CAGGCCGACT GGGAGTCCCC AGAACGTCGC CAGTCCAGGT CACCCAGTGA AGGAAGGTCA 
CAGACCCCCA ATAGACAGGG TACCGGTTCC CTAAGTGAGA GCTCCATCCC CTCTATTTCC 
GACACCAGCA CCCCAAGACG AAGCCGTCGC CAGCTCCCAC CTGTGCCACC AAAGCCTCGT 
CCCCTCCTCT CCTACAGCTC CCTGATGAGA CACACTGGCG GCATCTCTCC ACCTCCTGAT 
GGAAGTGAGG GTGGATCCCC ACTGGCCTCT CAAGCTCTGG AGAGCAACAG CGCTTGCCTG 
ACCGAGTCTT CCAACTCCTT GCACCCCCAG CAGGGCCAGC ATCCTTCCCC ACAGCACTAC 
ATCTCCGAGC CCTATCTGGC CCTCCATGAA GACTCCCACG CCTCAGACTG TGGCGAGGAG 
GAGACACTCA CCTTTGAGGC AGCCGTAGCC ACTAGCTTGG GCCGGTCCAA TACCATTGGC 
TCTGCGCCAC CCCTACGGCA CAGCTGGCAG ATGCCTAATG GGCACTATCG GCGGCGGAGG 
CGTGGGGGGC CTGGGCCTGG CATGATGTGT GGAGCTGTCA GTGACCTCCT GAGTGACACG 
GAAGAAGACG ATAAGTGCTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cacna1e ( XM_017312594.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017168083.1 Mus musculus calcium channel, voltage-dependent, R type, alpha 1E subunit (Cacna1e), transcript variant X3, mRNA.

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CloneID	OMu67260
Clone ID Related Accession (Same CDS sequence)	XM_017312578.1 , XM_030243926.1 , XM_017312594.1 , XM_030243929.1 , XM_011247909.2 , XM_017312578.2 , XM_011247909.3
Accession Version	XM_017312594.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7401bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	voltage-dependent R-type calcium channel subunit alpha-1E isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGCCCG AGATGCGTGG ACTGAGCCCA GGCCGAAGGC TGCCCGAGCC CACCCTGTGC 
CCGGCTACAG GAGGGAGCCC TGAGCCCGGG GCTGCTGGCA GGCGCCGCCA CGTCCTGGCT 
CTGCCTGATG AGGAGGCATG GCGGCCGCAG CAGTGCGCCC TGCTCGAGGC CAATGCCTCC 
GACGAGGTGG GCATGTTGCC TGCTTCCCCG CGCTCCGCGG TGGCCGGCTT CGACAACTTC 
TTCCCGGTGC AGGAAGGCGA GGGCCCGGGC TGGGAGGGCA GCACCACGCT GGATGCAGGC 
TCCAGCCCCT TCCTTCCTGT GAGCCCAGAG GTCATGAAGC GGAGGCGAGG GGGGCTCATC 
GAGCAGCGGG ACATCATCAA GGCCCACGAG GCACACAAGA TGCAGAGCAC CCCGCAGGCC 
CGGCGCAAGG AGTGGGAGAT GGCTCGCTTC GGGGAGGCGG TGGTCGTTGG CAGGCCAGGC 
TCAGGCGATG GAGACTCGGA CCAGAGCAGG AACCGACAAG GAACCCCCGT CCCGGCCTCG 
GGGCCGGCGG CCGCCTACAA GCAGTCAAAA GCGCAGAGGG CGCGGACTAT GGCTTTGTAC 
AACCCCATAC CAGTCCGGCA GAACTGTTTC ACGGTCAACA GATCCCTGTT CATCTTCGGA 
GAAGATAACA TTGTCAGGAA ATACGCCAAG AAGCTCATCG ATTGGCCGCC ATTTGAGTAC 
ATGATCCTGG CCACCATCAT TGCCAACTGC ATCGTCTTGG CCCTGGAGCA GCATCTTCCT 
GAGGATGACA AGACCCCAAT GTCTCGAAGA CTGGAGAAGA CAGAACCATA TTTCATTGGG 
ATCTTCTGCT TTGAAGCTGG GATCAAAATT GTGGCTCTAG GGTTCATCTT CCATAAGGGT 
TCATACCTCC GTAATGGCTG GAATGTCATG GACTTCATCG TGGTCCTTAG TGGCATCCTG 
GCCACTGCGG GAACCCACTT CAACACCCAC GTGGACCTGC GGACCCTCCG GGCTGTGCGT 
GTCCTGAGGC CTTTAAAGCT GGTATCAGGA ATACCTAGCC TGCAGATCGT GCTGAAGTCT 
ATCATGAAGG CCATGGTGCC TCTTCTGCAG ATTGGCCTTC TGCTCTTCTT TGCCATCCTC 
ATGTTTGCTA TCATTGGCTT GGAGTTCTAC AGTGGCAAGT TGCATCGAGC ATGCTTCATG 
AACAATTCAG GTATTCTAGA GGGGTTTGAT CCTCCTCACC CGTGTGGTGT GCAAGGCTGC 
CCGGCTGGTT ATGAATGTAA GGACTGGATC GGCCCCAATG ACGGGATCAC CCAGTTTGAC 
AACATCCTTT TTGCCGTGCT GACTGTCTTC CAATGCATCA CTATGGAAGG GTGGACCACT 
GTGCTGTACA ATACCAATGA TGCCTTAGGA GCCACCTGGA ACTGGCTGTA CTTCATCCCT 
CTCATCATCA TCGGATCCTT CTTTGTTCTC AACCTCGTCC TGGGAGTGCT TTCCGGGGAA 
TTTGCCAAAG AAAGAGAGAG AGTGGAGAAC CGAAGAGCGT TCATGAAGCT CCGGCGCCAG 
CAACAGATTG AGCGAGAATT GAACGGCTAC CGTGCCTGGA TAGACAAAGC AGAGGAAGTC 
ATGCTTGCTG AAGAAAATAA AAACTCGGGA ACATCAGCCT TGGAAGTGCT TCGAAGGGCA 
ACCATCAAAA GGAGCCGGAC GGAGGCCATG ACCCGAGACT CCAGCGATGA GCACTGCGTT 
GATATCTCTT CAGTGGGCAC ACCTCTTGCC AGAGCCAGTA TCAAGAGCAC AAAAGTAGAT 
GGGGCCTCCT ATTTCCGGCA CAAGGAACGG CTTCTGCGCA TCTCTATCCG CCACATGGTC 
AAGTCCCAAG TCTTTTACTG GATCGTCCTG AGTGTTGTGG CTCTTAATAC TGCCTGTGTG 
GCCATTGTTC ATCACAACCA GCCACAGTGG CTCACTCACC TCCTCTACTA TGCAGAGTTT 
TTGTTTCTGG GACTCTTCCT GTTGGAGATG TCCCTGAAGA TGTATGGCAT GGGGCCACGC 
CTCTATTTTC ACTCATCCTT CAACTGCTTT GATTTTGGGG TCACAGTGGG CAGCATCTTT 
GAGGTTGTCT GGGCAATCTT CAGACCTGGC ACATCTTTTG GAATCAGTGT CTTACGAGCT 
CTCCGGCTCC TAAGGATATT TAAAATAACC AAGTATTGGG CATCCCTCCG GAATCTGGTT 
GTCTCCTTGA TGAGCTCCAT GAAGTCTATC ATCAGCTTAC TCTTCCTTCT CTTCCTCTTC 
ATCGTTGTCT TTGCTCTCCT GGGAATGCAG CTCTTTGGAG GCAGGTTTAA CTTTAATGAT 
GGGACTCCTT CAGCTAATTT TGACACCTTC CCTGCAGCTA TCATGACAGT GTTCCAGATC 
CTGACAGGGG AAGACTGGAA TGAGGTGATG TACAATGGCA TTCGCTCCCA GGGTGGGGTC 
AGCTCAGGCA TGTGGTCTGC CATCTACTTC ATTGTGCTCA CCTTGTTTGG AAACTACACG 
CTGCTAAATG TATTCTTGGC CATCGCTGTG GACAATCTGG CCAATGCCCA GGAACTGACC 
AAGGATGAGC AGGAGGAAGA AGAAGCCTTC AACCAGAAAC ATGCACTGCA GAAGGCCAAG 
GAGGTCAGCC CGATGTCTGC TCCCAACATG CCTTCCATTG AAAGAGACAG AAGGAGAAGA 
CACCACATGT CGATGTGGGA GCCACGCAGC AGCCACCTGA GGGAGCGGAG GCGCCGGCAC 
CACATGTCTG TGTGGGAACA ACGCACCAGC CAGCTGAGGA GGCACATGCA AATGTCTAGC 
CAAGAAGCCC TCAACAAAGA GGAGGCCCCG CCAATGAACC CCCTCAATCC ACTCAACCCA 
CTGAGTCCTC TCAACCCCCT CAATGCTCAC CCCAGCCTTT ATCGGAGGCC CAGGCCCATT 
GAGGGCCTAG CCCTAGGTCT GGGTCTTGAA AAGTGTGAAG AAGAACGCAT CAGTCGTGGG 
GGCTCCCTCA AGGGGGACAT AGGGGGCCTG ACTAGCGCCC TGGACAACCA GAGGAGCCCC 
TTGTCCTTGG GCAAACGGGA GCCACCGTGG CTGCCTAGAT CCTGTCATGG AAACTGTGAC 
CCAATCCAGC AGGAGGCTGG GGGAGGAGAG ACTGTGGTGA CTTTTGAGGA CCGGGCCAGG 
CACAGGCAGA GTCAGAGGCG AAGCCGGCAT CGCCGAGTCA GGACTGAGGG CAAGGACTCT 
GCCTCTGCAT CCCGAAGCAG GTCTGCCAGC CAGGAGCGGA GTCTGGATGA AGGGGTGTCC 
GTTGAGGGGG AGAAGGAACA TGAGCCTCAC AGCAGCCACA GGAGCAAAGA GCCGACCATC 
CACGAAGAGG AGAGAACCCA GGACTTAAGG AGGACCAACA GTCTGATGGT CCCCAGGGGC 
TCTGGACTGG TAGGGGCTCT TGATGAAGCA GAAACACCTC TAGTCCAGCC CCAACCTGAG 
CTGGAAGTGG GGAAGGATGC AGCTCTGACA GAGCAGGAGG CTGAAGGCAG CAGTGAACAA 
GCCCTTCTTG GGGACGTACA GCTGGATGTG GGCCGGGGCA TTAGCCAGAG TGAACCGGAT 
CTCTCCTGCA TGACAGCCAA CATGGACAAG GCTACCACGG AGAGCACCAG TGTCACGGTC 
GCCATCCCTG ATGTGGACCC CTTGGTGGAC TCGACAGTGG TGAACATTAG CAACAAGACG 
GATGGAGAAG CCAGTCCCTT GAAGGAGGCA GAGACTAAAG AGGAAGAAGA AGAGGTAGAG 
AAGAAGAAGA AGCAGAAGAA GGAGAAGCGA GAGACTGGAA AAGCCATGGT GCCCCATAGC 
TCTATGTTCA TCTTCAGCAC CACTAACCCG ATCCGCAGGG CCTGCCACTA CATTGTGAAC 
CTACGCTACT TTGAAATGTG CATTCTCTTG GTGATTGCGG CCAGCAGCAT TGCCCTGGCA 
GCCGAGGACC CTGTACTAAC TAACTCGGAG CGTAACAAAG TCCTGAGATA TTTCGACTAT 
GTTTTCACAG GAGTGTTTAC CTTTGAGATG GTTATAAAGA TGATAGACCA AGGCTTGATC 
CTGCAGGATG GATCATACTT CAGAGACCTC TGGAACATCC TGGACTTTGT AGTAGTGGTT 
GGTGCATTGG TAGCATTTGC TCTGGCGAAC GCTTTGGGAA CCAACAAGGG GAGGGATATC 
AAGACTATCA AGTCTCTGAG GGTACTTCGA GTTCTGCGGC CACTGAAAAC CATCAAGCGC 
TTGCCCAAGC TGAAGGCTGT GTTTGACTGC GTGGTGACCT CCTTGAAGAA TGTCTTCAAC 
ATACTCATTG TCTACAAGCT CTTCATGTTC ATCTTTGCCG TCATCGCTGT TCAGCTATTC 
AAAGGAAAGT TCTTTTATTG CACTGACAGT TCCAAGGACA CAGAGAAGGA GTGCATAGGC 
AACTATGTAG ACCACGAGAA AAACAAGATG GAGGTGAAGG GCCGGGAATG GAAGCGTCAT 
GAATTCCACT ATGACAACAT CATCTGGGCC CTGCTTACCC TCTTCACAGT CTCTACAGGG 
GAAGGATGGC CTCAGGTTCT GCAGCACTCT GTAGATGTGA CAGAGGAGGA CAGAGGCCCA 
AGTCGCAGCA ACCGCATGGA GATGTCCATC TTTTATGTGG TCTACTTTGT GGTCTTCCCT 
TTCTTCTTTG TCAATATCTT CGTGGCTCTC ATTATCATCA CCTTCCAGGA GCAAGGGGAC 
AAGATGATGG AGGAGTGTAG CCTGGAGAAG AATGAGCGGG CATGCATCGA CTTCGCCATT 
AGTGCCAAAC CTCTCACCCG CTACATGCCA CAGAACAGGC ACACCTTCCA GTACCGTGTG 
TGGCACTTTG TAGTCTCCCC ATCCTTTGAG TATACCATCA TGGCCATGAT TGCCTTGAAC 
ACTGTCGTGC TGATGATGAA GTACTACTCT GCTCCTTGCA CCTATGAGCT CGCCTTAAAG 
TACCTGAACA TCGCCTTCAC TATGGTGTTT TCCCTGGAGT GTGTCCTAAA GGTCATTGCT 
TTCGGCTTCC TGAACTATTT CCGAGATACC TGGAACATCT TTGACTTCAT CACGGTGATC 
GGCAGTATCA CAGAAATTAT CCTGACAGAT AGCAAGCTGG TGAACACCAG TGGCTTCAAT 
ATGAGCTTTC TGAAGCTCTT CCGAGCTGCA CGGCTCATAA AGCTTCTGAG ACAGGGCTAC 
ACCATCCGCA TCTTACTGTG GACCTTCGTG CAGTCCTTTA AGGCTCTTCC CTACGTCTGC 
CTTCTCATTG CCATGCTTTT CTTCATCTAC GCCATCATTG GGATGCAGGT GTTTGGAAAC 
ATAAAGTTAG ATGAGGAGAG TCACATCAAC CGGCACAACA ACTTCCGGAG TTTCTTTGGA 
TCCCTCATGC TGCTTTTCAG GAGTGCCACG GGGGAGGCTT GGCAGGAGAT TATGCTGTCC 
TGTCTCGGTG AGAAGGGTTG TGAGCCTGAC ACCACTGCAC CCTCAGGGCA GAATGAGAGT 
GAGCGCTGTG GCACTGACCT GGCCTACGTT TATTTTGTCT CCTTCATCTT CTTCTGCTCC 
TTCCTGATGC TCAACCTGTT CGTGGCTGTC ATCATGGACA ACTTTGAGTA CCTGACTCGA 
GATTCCTCCA TCCTGGGGCC TCACCACTTA GATGAGTTTG TCCGTGTCTG GGCAGAATAT 
GACAGAGCAG CATGTGGCCG CATCCATTAC ACTGAGATGT ATGAAATGCT GACTCTCATG 
TCACCACCGC TAGGCCTCGG CAAGAGATGT CCCTCCAAAG TGGCCTATAA GAGGCTGGTC 
CTGATGAACA TGCCAGTGGC TGAGGACATG ACAGTCCACT TCACCTCCAC ACTTATGGCT 
CTGATCCGGA CAGCTCTGGA CATTAAAATC GCCAAAGGTG GTGCAGACAG ACAGCAGCTA 
GACTCGGAGC TGCAAAAAGA GACCCTGGCC ATTTGGCCTC ACCTGTCCCA AAAGATGTTG 
GATCTGCTTG TGCCCATGCC CAAAGCCTCT GACCTGACTG TGGGCAAGAT CTATGCAGCA 
ATGATGATCA TGGACTACTA TAAACAGAGT AAGGTAAAGA AACAGAGACA GCAGCTGGAA 
GAACAGAAAA ATGCACCCAT GTTCCAGCGC ATGGAGCCCT CATCACTGCC TCAGGAGATC 
ATTGCTAATG CCAAAGCCCT GCCTTATCTC CAGCAGGACC CTGTTTCTGG CCTGAGTGGT 
CGGAGTGGAT ACCCTTCAAT GAGTCCACTC TCCCCTCAAG AAATATTCCA GTTGGCTTGT 
ATGGACCCAG CGGATGATGG ACAATTCCAG GAACAACAAT CTCTGGTGAA TGCACAAGAG 
CCAGAGGTTA GTGAATTAAA AAGTGTGCAG TCCTCTAACC ATGGCATCTA CCTTCCTCCG 
GACACCCAGG AGCATGCGGG ATCTGGGAGG GCATCCTCTA TGCCACGTCT GACTATGGAT 
CCCCAGGTGG TAACAGACCC TAGCTCTATG CGACGTTCAT TTTCTACAAT TCGGGATAAG 
CGTTCAAATT CCTCCTGGTT GGAGGAGTTC TCCATGGAAC GCAGCAGTGA AAACACTTAC 
AAGTCGCGGC GCAGAAGTTA CCATTCCTCC CTGAGGCTGT CAGCACACCG CCTGAATTCT 
GACTCTGGTC ACAAGTCTGA CACTCACCGA TCAGGAGGCA GGGAGAGAGG CCGATCCAAA 
GAACGAAAGC ACCTCCTCTC TCCAGATGTC TCCCGCTGCA ATTCTGAAGA GCGAGGGACC 
CAGGCCGACT GGGAGTCCCC AGAACGTCGC CAGTCCAGGT CACCCAGTGA AGGAAGGTCA 
CAGACCCCCA ATAGACAGGG TACCGGTTCC CTAAGTGAGA GCTCCATCCC CTCTATTTCC 
GACACCAGCA CCCCAAGACG AAGCCGTCGC CAGCTCCCAC CTGTGCCACC AAAGCCTCGT 
CCCCTCCTCT CCTACAGCTC CCTGATGAGA CACACTGGCG GCATCTCTCC ACCTCCTGAT 
GGAAGTGAGG GTGGATCCCC ACTGGCCTCT CAAGCTCTGG AGAGCAACAG CGCTTGCCTG 
ACCGAGTCTT CCAACTCCTT GCACCCCCAG CAGGGCCAGC ATCCTTCCCC ACAGCACTAC 
ATCTCCGAGC CCTATCTGGC CCTCCATGAA GACTCCCACG CCTCAGACTG TGGCGAGGAG 
GAGACACTCA CCTTTGAGGC AGCCGTAGCC ACTAGCTTGG GCCGGTCCAA TACCATTGGC 
TCTGCGCCAC CCCTACGGCA CAGCTGGCAG ATGCCTAATG GGCACTATCG GCGGCGGAGG 
CGTGGGGGGC CTGGGCCTGG CATGATGTGT GGAGCTGTCA GTGACCTCCT GAGTGACACG 
GAAGAAGACG ATAAGTGCTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017168083.1
CDS	36..7436
Translation	MVPEMRGLSPGRRLPEPTLCPATGGSPEPGAAGRRRHVLALPDEEAWRPQQCALLEANASDEVGMLPASPRSAVAGFDNFFPVQEGEGPGWEGSTTLDAGSSPFLPVSPEVMKRRRGGLIEQRDIIKAHEAHKMQSTPQARRKEWEMARFGEAVVVGRPGSGDGDSDQSRNRQGTPVPASGPAAAYKQSKAQRARTMALYNPIPVRQNCFTVNRSLFIFGEDNIVRKYAKKLIDWPPFEYMILATIIANCIVLALEQHLPEDDKTPMSRRLEKTEPYFIGIFCFEAGIKIVALGFIFHKGSYLRNGWNVMDFIVVLSGILATAGTHFNTHVDLRTLRAVRVLRPLKLVSGIPSLQIVLKSIMKAMVPLLQIGLLLFFAILMFAIIGLEFYSGKLHRACFMNNSGILEGFDPPHPCGVQGCPAGYECKDWIGPNDGITQFDNILFAVLTVFQCITMEGWTTVLYNTNDALGATWNWLYFIPLIIIGSFFVLNLVLGVLSGEFAKERERVENRRAFMKLRRQQQIERELNGYRAWIDKAEEVMLAEENKNSGTSALEVLRRATIKRSRTEAMTRDSSDEHCVDISSVGTPLARASIKSTKVDGASYFRHKERLLRISIRHMVKSQVFYWIVLSVVALNTACVAIVHHNQPQWLTHLLYYAEFLFLGLFLLEMSLKMYGMGPRLYFHSSFNCFDFGVTVGSIFEVVWAIFRPGTSFGISVLRALRLLRIFKITKYWASLRNLVVSLMSSMKSIISLLFLLFLFIVVFALLGMQLFGGRFNFNDGTPSANFDTFPAAIMTVFQILTGEDWNEVMYNGIRSQGGVSSGMWSAIYFIVLTLFGNYTLLNVFLAIAVDNLANAQELTKDEQEEEEAFNQKHALQKAKEVSPMSAPNMPSIERDRRRRHHMSMWEPRSSHLRERRRRHHMSVWEQRTSQLRRHMQMSSQEALNKEEAPPMNPLNPLNPLSPLNPLNAHPSLYRRPRPIEGLALGLGLEKCEEERISRGGSLKGDIGGLTSALDNQRSPLSLGKREPPWLPRSCHGNCDPIQQEAGGGETVVTFEDRARHRQSQRRSRHRRVRTEGKDSASASRSRSASQERSLDEGVSVEGEKEHEPHSSHRSKEPTIHEEERTQDLRRTNSLMVPRGSGLVGALDEAETPLVQPQPELEVGKDAALTEQEAEGSSEQALLGDVQLDVGRGISQSEPDLSCMTANMDKATTESTSVTVAIPDVDPLVDSTVVNISNKTDGEASPLKEAETKEEEEEVEKKKKQKKEKRETGKAMVPHSSMFIFSTTNPIRRACHYIVNLRYFEMCILLVIAASSIALAAEDPVLTNSERNKVLRYFDYVFTGVFTFEMVIKMIDQGLILQDGSYFRDLWNILDFVVVVGALVAFALANALGTNKGRDIKTIKSLRVLRVLRPLKTIKRLPKLKAVFDCVVTSLKNVFNILIVYKLFMFIFAVIAVQLFKGKFFYCTDSSKDTEKECIGNYVDHEKNKMEVKGREWKRHEFHYDNIIWALLTLFTVSTGEGWPQVLQHSVDVTEEDRGPSRSNRMEMSIFYVVYFVVFPFFFVNIFVALIIITFQEQGDKMMEECSLEKNERACIDFAISAKPLTRYMPQNRHTFQYRVWHFVVSPSFEYTIMAMIALNTVVLMMKYYSAPCTYELALKYLNIAFTMVFSLECVLKVIAFGFLNYFRDTWNIFDFITVIGSITEIILTDSKLVNTSGFNMSFLKLFRAARLIKLLRQGYTIRILLWTFVQSFKALPYVCLLIAMLFFIYAIIGMQVFGNIKLDEESHINRHNNFRSFFGSLMLLFRSATGEAWQEIMLSCLGEKGCEPDTTAPSGQNESERCGTDLAYVYFVSFIFFCSFLMLNLFVAVIMDNFEYLTRDSSILGPHHLDEFVRVWAEYDRAACGRIHYTEMYEMLTLMSPPLGLGKRCPSKVAYKRLVLMNMPVAEDMTVHFTSTLMALIRTALDIKIAKGGADRQQLDSELQKETLAIWPHLSQKMLDLLVPMPKASDLTVGKIYAAMMIMDYYKQSKVKKQRQQLEEQKNAPMFQRMEPSSLPQEIIANAKALPYLQQDPVSGLSGRSGYPSMSPLSPQEIFQLACMDPADDGQFQEQQSLVNAQEPEVSELKSVQSSNHGIYLPPDTQEHAGSGRASSMPRLTMDPQVVTDPSSMRRSFSTIRDKRSNSSWLEEFSMERSSENTYKSRRRSYHSSLRLSAHRLNSDSGHKSDTHRSGGRERGRSKERKHLLSPDVSRCNSEERGTQADWESPERRQSRSPSEGRSQTPNRQGTGSLSESSIPSISDTSTPRRSRRQLPPVPPKPRPLLSYSSLMRHTGGISPPPDGSEGGSPLASQALESNSACLTESSNSLHPQQGQHPSPQHYISEPYLALHEDSHASDCGEEETLTFEAAVATSLGRSNTIGSAPPLRHSWQMPNGHYRRRRRGGPGPGMMCGAVSDLLSDTEEDDKC

Target ORF information:

RefSeq Version	XM_017312594.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus calcium channel, voltage-dependent, R type, alpha 1E subunit (Cacna1e), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017312594.1

ORF Insert Sequence:

ATGGTGCCCG AGATGCGTGG ACTGAGCCCA GGCCGAAGGC TGCCCGAGCC CACCCTGTGC 
CCGGCTACAG GAGGGAGCCC TGAGCCCGGG GCTGCTGGCA GGCGCCGCCA CGTCCTGGCT 
CTGCCTGATG AGGAGGCATG GCGGCCGCAG CAGTGCGCCC TGCTCGAGGC CAATGCCTCC 
GACGAGGTGG GCATGTTGCC TGCTTCCCCG CGCTCCGCGG TGGCCGGCTT CGACAACTTC 
TTCCCGGTGC AGGAAGGCGA GGGCCCGGGC TGGGAGGGCA GCACCACGCT GGATGCAGGC 
TCCAGCCCCT TCCTTCCTGT GAGCCCAGAG GTCATGAAGC GGAGGCGAGG GGGGCTCATC 
GAGCAGCGGG ACATCATCAA GGCCCACGAG GCACACAAGA TGCAGAGCAC CCCGCAGGCC 
CGGCGCAAGG AGTGGGAGAT GGCTCGCTTC GGGGAGGCGG TGGTCGTTGG CAGGCCAGGC 
TCAGGCGATG GAGACTCGGA CCAGAGCAGG AACCGACAAG GAACCCCCGT CCCGGCCTCG 
GGGCCGGCGG CCGCCTACAA GCAGTCAAAA GCGCAGAGGG CGCGGACTAT GGCTTTGTAC 
AACCCCATAC CAGTCCGGCA GAACTGTTTC ACGGTCAACA GATCCCTGTT CATCTTCGGA 
GAAGATAACA TTGTCAGGAA ATACGCCAAG AAGCTCATCG ATTGGCCGCC ATTTGAGTAC 
ATGATCCTGG CCACCATCAT TGCCAACTGC ATCGTCTTGG CCCTGGAGCA GCATCTTCCT 
GAGGATGACA AGACCCCAAT GTCTCGAAGA CTGGAGAAGA CAGAACCATA TTTCATTGGG 
ATCTTCTGCT TTGAAGCTGG GATCAAAATT GTGGCTCTAG GGTTCATCTT CCATAAGGGT 
TCATACCTCC GTAATGGCTG GAATGTCATG GACTTCATCG TGGTCCTTAG TGGCATCCTG 
GCCACTGCGG GAACCCACTT CAACACCCAC GTGGACCTGC GGACCCTCCG GGCTGTGCGT 
GTCCTGAGGC CTTTAAAGCT GGTATCAGGA ATACCTAGCC TGCAGATCGT GCTGAAGTCT 
ATCATGAAGG CCATGGTGCC TCTTCTGCAG ATTGGCCTTC TGCTCTTCTT TGCCATCCTC 
ATGTTTGCTA TCATTGGCTT GGAGTTCTAC AGTGGCAAGT TGCATCGAGC ATGCTTCATG 
AACAATTCAG GTATTCTAGA GGGGTTTGAT CCTCCTCACC CGTGTGGTGT GCAAGGCTGC 
CCGGCTGGTT ATGAATGTAA GGACTGGATC GGCCCCAATG ACGGGATCAC CCAGTTTGAC 
AACATCCTTT TTGCCGTGCT GACTGTCTTC CAATGCATCA CTATGGAAGG GTGGACCACT 
GTGCTGTACA ATACCAATGA TGCCTTAGGA GCCACCTGGA ACTGGCTGTA CTTCATCCCT 
CTCATCATCA TCGGATCCTT CTTTGTTCTC AACCTCGTCC TGGGAGTGCT TTCCGGGGAA 
TTTGCCAAAG AAAGAGAGAG AGTGGAGAAC CGAAGAGCGT TCATGAAGCT CCGGCGCCAG 
CAACAGATTG AGCGAGAATT GAACGGCTAC CGTGCCTGGA TAGACAAAGC AGAGGAAGTC 
ATGCTTGCTG AAGAAAATAA AAACTCGGGA ACATCAGCCT TGGAAGTGCT TCGAAGGGCA 
ACCATCAAAA GGAGCCGGAC GGAGGCCATG ACCCGAGACT CCAGCGATGA GCACTGCGTT 
GATATCTCTT CAGTGGGCAC ACCTCTTGCC AGAGCCAGTA TCAAGAGCAC AAAAGTAGAT 
GGGGCCTCCT ATTTCCGGCA CAAGGAACGG CTTCTGCGCA TCTCTATCCG CCACATGGTC 
AAGTCCCAAG TCTTTTACTG GATCGTCCTG AGTGTTGTGG CTCTTAATAC TGCCTGTGTG 
GCCATTGTTC ATCACAACCA GCCACAGTGG CTCACTCACC TCCTCTACTA TGCAGAGTTT 
TTGTTTCTGG GACTCTTCCT GTTGGAGATG TCCCTGAAGA TGTATGGCAT GGGGCCACGC 
CTCTATTTTC ACTCATCCTT CAACTGCTTT GATTTTGGGG TCACAGTGGG CAGCATCTTT 
GAGGTTGTCT GGGCAATCTT CAGACCTGGC ACATCTTTTG GAATCAGTGT CTTACGAGCT 
CTCCGGCTCC TAAGGATATT TAAAATAACC AAGTATTGGG CATCCCTCCG GAATCTGGTT 
GTCTCCTTGA TGAGCTCCAT GAAGTCTATC ATCAGCTTAC TCTTCCTTCT CTTCCTCTTC 
ATCGTTGTCT TTGCTCTCCT GGGAATGCAG CTCTTTGGAG GCAGGTTTAA CTTTAATGAT 
GGGACTCCTT CAGCTAATTT TGACACCTTC CCTGCAGCTA TCATGACAGT GTTCCAGATC 
CTGACAGGGG AAGACTGGAA TGAGGTGATG TACAATGGCA TTCGCTCCCA GGGTGGGGTC 
AGCTCAGGCA TGTGGTCTGC CATCTACTTC ATTGTGCTCA CCTTGTTTGG AAACTACACG 
CTGCTAAATG TATTCTTGGC CATCGCTGTG GACAATCTGG CCAATGCCCA GGAACTGACC 
AAGGATGAGC AGGAGGAAGA AGAAGCCTTC AACCAGAAAC ATGCACTGCA GAAGGCCAAG 
GAGGTCAGCC CGATGTCTGC TCCCAACATG CCTTCCATTG AAAGAGACAG AAGGAGAAGA 
CACCACATGT CGATGTGGGA GCCACGCAGC AGCCACCTGA GGGAGCGGAG GCGCCGGCAC 
CACATGTCTG TGTGGGAACA ACGCACCAGC CAGCTGAGGA GGCACATGCA AATGTCTAGC 
CAAGAAGCCC TCAACAAAGA GGAGGCCCCG CCAATGAACC CCCTCAATCC ACTCAACCCA 
CTGAGTCCTC TCAACCCCCT CAATGCTCAC CCCAGCCTTT ATCGGAGGCC CAGGCCCATT 
GAGGGCCTAG CCCTAGGTCT GGGTCTTGAA AAGTGTGAAG AAGAACGCAT CAGTCGTGGG 
GGCTCCCTCA AGGGGGACAT AGGGGGCCTG ACTAGCGCCC TGGACAACCA GAGGAGCCCC 
TTGTCCTTGG GCAAACGGGA GCCACCGTGG CTGCCTAGAT CCTGTCATGG AAACTGTGAC 
CCAATCCAGC AGGAGGCTGG GGGAGGAGAG ACTGTGGTGA CTTTTGAGGA CCGGGCCAGG 
CACAGGCAGA GTCAGAGGCG AAGCCGGCAT CGCCGAGTCA GGACTGAGGG CAAGGACTCT 
GCCTCTGCAT CCCGAAGCAG GTCTGCCAGC CAGGAGCGGA GTCTGGATGA AGGGGTGTCC 
GTTGAGGGGG AGAAGGAACA TGAGCCTCAC AGCAGCCACA GGAGCAAAGA GCCGACCATC 
CACGAAGAGG AGAGAACCCA GGACTTAAGG AGGACCAACA GTCTGATGGT CCCCAGGGGC 
TCTGGACTGG TAGGGGCTCT TGATGAAGCA GAAACACCTC TAGTCCAGCC CCAACCTGAG 
CTGGAAGTGG GGAAGGATGC AGCTCTGACA GAGCAGGAGG CTGAAGGCAG CAGTGAACAA 
GCCCTTCTTG GGGACGTACA GCTGGATGTG GGCCGGGGCA TTAGCCAGAG TGAACCGGAT 
CTCTCCTGCA TGACAGCCAA CATGGACAAG GCTACCACGG AGAGCACCAG TGTCACGGTC 
GCCATCCCTG ATGTGGACCC CTTGGTGGAC TCGACAGTGG TGAACATTAG CAACAAGACG 
GATGGAGAAG CCAGTCCCTT GAAGGAGGCA GAGACTAAAG AGGAAGAAGA AGAGGTAGAG 
AAGAAGAAGA AGCAGAAGAA GGAGAAGCGA GAGACTGGAA AAGCCATGGT GCCCCATAGC 
TCTATGTTCA TCTTCAGCAC CACTAACCCG ATCCGCAGGG CCTGCCACTA CATTGTGAAC 
CTACGCTACT TTGAAATGTG CATTCTCTTG GTGATTGCGG CCAGCAGCAT TGCCCTGGCA 
GCCGAGGACC CTGTACTAAC TAACTCGGAG CGTAACAAAG TCCTGAGATA TTTCGACTAT 
GTTTTCACAG GAGTGTTTAC CTTTGAGATG GTTATAAAGA TGATAGACCA AGGCTTGATC 
CTGCAGGATG GATCATACTT CAGAGACCTC TGGAACATCC TGGACTTTGT AGTAGTGGTT 
GGTGCATTGG TAGCATTTGC TCTGGCGAAC GCTTTGGGAA CCAACAAGGG GAGGGATATC 
AAGACTATCA AGTCTCTGAG GGTACTTCGA GTTCTGCGGC CACTGAAAAC CATCAAGCGC 
TTGCCCAAGC TGAAGGCTGT GTTTGACTGC GTGGTGACCT CCTTGAAGAA TGTCTTCAAC 
ATACTCATTG TCTACAAGCT CTTCATGTTC ATCTTTGCCG TCATCGCTGT TCAGCTATTC 
AAAGGAAAGT TCTTTTATTG CACTGACAGT TCCAAGGACA CAGAGAAGGA GTGCATAGGC 
AACTATGTAG ACCACGAGAA AAACAAGATG GAGGTGAAGG GCCGGGAATG GAAGCGTCAT 
GAATTCCACT ATGACAACAT CATCTGGGCC CTGCTTACCC TCTTCACAGT CTCTACAGGG 
GAAGGATGGC CTCAGGTTCT GCAGCACTCT GTAGATGTGA CAGAGGAGGA CAGAGGCCCA 
AGTCGCAGCA ACCGCATGGA GATGTCCATC TTTTATGTGG TCTACTTTGT GGTCTTCCCT 
TTCTTCTTTG TCAATATCTT CGTGGCTCTC ATTATCATCA CCTTCCAGGA GCAAGGGGAC 
AAGATGATGG AGGAGTGTAG CCTGGAGAAG AATGAGCGGG CATGCATCGA CTTCGCCATT 
AGTGCCAAAC CTCTCACCCG CTACATGCCA CAGAACAGGC ACACCTTCCA GTACCGTGTG 
TGGCACTTTG TAGTCTCCCC ATCCTTTGAG TATACCATCA TGGCCATGAT TGCCTTGAAC 
ACTGTCGTGC TGATGATGAA GTACTACTCT GCTCCTTGCA CCTATGAGCT CGCCTTAAAG 
TACCTGAACA TCGCCTTCAC TATGGTGTTT TCCCTGGAGT GTGTCCTAAA GGTCATTGCT 
TTCGGCTTCC TGAACTATTT CCGAGATACC TGGAACATCT TTGACTTCAT CACGGTGATC 
GGCAGTATCA CAGAAATTAT CCTGACAGAT AGCAAGCTGG TGAACACCAG TGGCTTCAAT 
ATGAGCTTTC TGAAGCTCTT CCGAGCTGCA CGGCTCATAA AGCTTCTGAG ACAGGGCTAC 
ACCATCCGCA TCTTACTGTG GACCTTCGTG CAGTCCTTTA AGGCTCTTCC CTACGTCTGC 
CTTCTCATTG CCATGCTTTT CTTCATCTAC GCCATCATTG GGATGCAGGT GTTTGGAAAC 
ATAAAGTTAG ATGAGGAGAG TCACATCAAC CGGCACAACA ACTTCCGGAG TTTCTTTGGA 
TCCCTCATGC TGCTTTTCAG GAGTGCCACG GGGGAGGCTT GGCAGGAGAT TATGCTGTCC 
TGTCTCGGTG AGAAGGGTTG TGAGCCTGAC ACCACTGCAC CCTCAGGGCA GAATGAGAGT 
GAGCGCTGTG GCACTGACCT GGCCTACGTT TATTTTGTCT CCTTCATCTT CTTCTGCTCC 
TTCCTGATGC TCAACCTGTT CGTGGCTGTC ATCATGGACA ACTTTGAGTA CCTGACTCGA 
GATTCCTCCA TCCTGGGGCC TCACCACTTA GATGAGTTTG TCCGTGTCTG GGCAGAATAT 
GACAGAGCAG CATGTGGCCG CATCCATTAC ACTGAGATGT ATGAAATGCT GACTCTCATG 
TCACCACCGC TAGGCCTCGG CAAGAGATGT CCCTCCAAAG TGGCCTATAA GAGGCTGGTC 
CTGATGAACA TGCCAGTGGC TGAGGACATG ACAGTCCACT TCACCTCCAC ACTTATGGCT 
CTGATCCGGA CAGCTCTGGA CATTAAAATC GCCAAAGGTG GTGCAGACAG ACAGCAGCTA 
GACTCGGAGC TGCAAAAAGA GACCCTGGCC ATTTGGCCTC ACCTGTCCCA AAAGATGTTG 
GATCTGCTTG TGCCCATGCC CAAAGCCTCT GACCTGACTG TGGGCAAGAT CTATGCAGCA 
ATGATGATCA TGGACTACTA TAAACAGAGT AAGGTAAAGA AACAGAGACA GCAGCTGGAA 
GAACAGAAAA ATGCACCCAT GTTCCAGCGC ATGGAGCCCT CATCACTGCC TCAGGAGATC 
ATTGCTAATG CCAAAGCCCT GCCTTATCTC CAGCAGGACC CTGTTTCTGG CCTGAGTGGT 
CGGAGTGGAT ACCCTTCAAT GAGTCCACTC TCCCCTCAAG AAATATTCCA GTTGGCTTGT 
ATGGACCCAG CGGATGATGG ACAATTCCAG GAACAACAAT CTCTGGTGAA TGCACAAGAG 
CCAGAGGTTA GTGAATTAAA AAGTGTGCAG TCCTCTAACC ATGGCATCTA CCTTCCTCCG 
GACACCCAGG AGCATGCGGG ATCTGGGAGG GCATCCTCTA TGCCACGTCT GACTATGGAT 
CCCCAGGTGG TAACAGACCC TAGCTCTATG CGACGTTCAT TTTCTACAAT TCGGGATAAG 
CGTTCAAATT CCTCCTGGTT GGAGGAGTTC TCCATGGAAC GCAGCAGTGA AAACACTTAC 
AAGTCGCGGC GCAGAAGTTA CCATTCCTCC CTGAGGCTGT CAGCACACCG CCTGAATTCT 
GACTCTGGTC ACAAGTCTGA CACTCACCGA TCAGGAGGCA GGGAGAGAGG CCGATCCAAA 
GAACGAAAGC ACCTCCTCTC TCCAGATGTC TCCCGCTGCA ATTCTGAAGA GCGAGGGACC 
CAGGCCGACT GGGAGTCCCC AGAACGTCGC CAGTCCAGGT CACCCAGTGA AGGAAGGTCA 
CAGACCCCCA ATAGACAGGG TACCGGTTCC CTAAGTGAGA GCTCCATCCC CTCTATTTCC 
GACACCAGCA CCCCAAGACG AAGCCGTCGC CAGCTCCCAC CTGTGCCACC AAAGCCTCGT 
CCCCTCCTCT CCTACAGCTC CCTGATGAGA CACACTGGCG GCATCTCTCC ACCTCCTGAT 
GGAAGTGAGG GTGGATCCCC ACTGGCCTCT CAAGCTCTGG AGAGCAACAG CGCTTGCCTG 
ACCGAGTCTT CCAACTCCTT GCACCCCCAG CAGGGCCAGC ATCCTTCCCC ACAGCACTAC 
ATCTCCGAGC CCTATCTGGC CCTCCATGAA GACTCCCACG CCTCAGACTG TGGCGAGGAG 
GAGACACTCA CCTTTGAGGC AGCCGTAGCC ACTAGCTTGG GCCGGTCCAA TACCATTGGC 
TCTGCGCCAC CCCTACGGCA CAGCTGGCAG ATGCCTAATG GGCACTATCG GCGGCGGAGG 
CGTGGGGGGC CTGGGCCTGG CATGATGTGT GGAGCTGTCA GTGACCTCCT GAGTGACACG 
GAAGAAGACG ATAAGTGCTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cacna1e ( XM_011247909.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011246211.1 Mus musculus calcium channel, voltage-dependent, R type, alpha 1E subunit (Cacna1e), transcript variant X2, mRNA.

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CloneID	OMu67260
Clone ID Related Accession (Same CDS sequence)	XM_017312578.1 , XM_030243926.1 , XM_017312594.1 , XM_030243929.1 , XM_011247909.2 , XM_017312578.2 , XM_011247909.3
Accession Version	XM_011247909.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7401bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	voltage-dependent R-type calcium channel subunit alpha-1E isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_011247909.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGCCCG AGATGCGTGG ACTGAGCCCA GGCCGAAGGC TGCCCGAGCC CACCCTGTGC 
CCGGCTACAG GAGGGAGCCC TGAGCCCGGG GCTGCTGGCA GGCGCCGCCA CGTCCTGGCT 
CTGCCTGATG AGGAGGCATG GCGGCCGCAG CAGTGCGCCC TGCTCGAGGC CAATGCCTCC 
GACGAGGTGG GCATGTTGCC TGCTTCCCCG CGCTCCGCGG TGGCCGGCTT CGACAACTTC 
TTCCCGGTGC AGGAAGGCGA GGGCCCGGGC TGGGAGGGCA GCACCACGCT GGATGCAGGC 
TCCAGCCCCT TCCTTCCTGT GAGCCCAGAG GTCATGAAGC GGAGGCGAGG GGGGCTCATC 
GAGCAGCGGG ACATCATCAA GGCCCACGAG GCACACAAGA TGCAGAGCAC CCCGCAGGCC 
CGGCGCAAGG AGTGGGAGAT GGCTCGCTTC GGGGAGGCGG TGGTCGTTGG CAGGCCAGGC 
TCAGGCGATG GAGACTCGGA CCAGAGCAGG AACCGACAAG GAACCCCCGT CCCGGCCTCG 
GGGCCGGCGG CCGCCTACAA GCAGTCAAAA GCGCAGAGGG CGCGGACTAT GGCTTTGTAC 
AACCCCATAC CAGTCCGGCA GAACTGTTTC ACGGTCAACA GATCCCTGTT CATCTTCGGA 
GAAGATAACA TTGTCAGGAA ATACGCCAAG AAGCTCATCG ATTGGCCGCC ATTTGAGTAC 
ATGATCCTGG CCACCATCAT TGCCAACTGC ATCGTCTTGG CCCTGGAGCA GCATCTTCCT 
GAGGATGACA AGACCCCAAT GTCTCGAAGA CTGGAGAAGA CAGAACCATA TTTCATTGGG 
ATCTTCTGCT TTGAAGCTGG GATCAAAATT GTGGCTCTAG GGTTCATCTT CCATAAGGGT 
TCATACCTCC GTAATGGCTG GAATGTCATG GACTTCATCG TGGTCCTTAG TGGCATCCTG 
GCCACTGCGG GAACCCACTT CAACACCCAC GTGGACCTGC GGACCCTCCG GGCTGTGCGT 
GTCCTGAGGC CTTTAAAGCT GGTATCAGGA ATACCTAGCC TGCAGATCGT GCTGAAGTCT 
ATCATGAAGG CCATGGTGCC TCTTCTGCAG ATTGGCCTTC TGCTCTTCTT TGCCATCCTC 
ATGTTTGCTA TCATTGGCTT GGAGTTCTAC AGTGGCAAGT TGCATCGAGC ATGCTTCATG 
AACAATTCAG GTATTCTAGA GGGGTTTGAT CCTCCTCACC CGTGTGGTGT GCAAGGCTGC 
CCGGCTGGTT ATGAATGTAA GGACTGGATC GGCCCCAATG ACGGGATCAC CCAGTTTGAC 
AACATCCTTT TTGCCGTGCT GACTGTCTTC CAATGCATCA CTATGGAAGG GTGGACCACT 
GTGCTGTACA ATACCAATGA TGCCTTAGGA GCCACCTGGA ACTGGCTGTA CTTCATCCCT 
CTCATCATCA TCGGATCCTT CTTTGTTCTC AACCTCGTCC TGGGAGTGCT TTCCGGGGAA 
TTTGCCAAAG AAAGAGAGAG AGTGGAGAAC CGAAGAGCGT TCATGAAGCT CCGGCGCCAG 
CAACAGATTG AGCGAGAATT GAACGGCTAC CGTGCCTGGA TAGACAAAGC AGAGGAAGTC 
ATGCTTGCTG AAGAAAATAA AAACTCGGGA ACATCAGCCT TGGAAGTGCT TCGAAGGGCA 
ACCATCAAAA GGAGCCGGAC GGAGGCCATG ACCCGAGACT CCAGCGATGA GCACTGCGTT 
GATATCTCTT CAGTGGGCAC ACCTCTTGCC AGAGCCAGTA TCAAGAGCAC AAAAGTAGAT 
GGGGCCTCCT ATTTCCGGCA CAAGGAACGG CTTCTGCGCA TCTCTATCCG CCACATGGTC 
AAGTCCCAAG TCTTTTACTG GATCGTCCTG AGTGTTGTGG CTCTTAATAC TGCCTGTGTG 
GCCATTGTTC ATCACAACCA GCCACAGTGG CTCACTCACC TCCTCTACTA TGCAGAGTTT 
TTGTTTCTGG GACTCTTCCT GTTGGAGATG TCCCTGAAGA TGTATGGCAT GGGGCCACGC 
CTCTATTTTC ACTCATCCTT CAACTGCTTT GATTTTGGGG TCACAGTGGG CAGCATCTTT 
GAGGTTGTCT GGGCAATCTT CAGACCTGGC ACATCTTTTG GAATCAGTGT CTTACGAGCT 
CTCCGGCTCC TAAGGATATT TAAAATAACC AAGTATTGGG CATCCCTCCG GAATCTGGTT 
GTCTCCTTGA TGAGCTCCAT GAAGTCTATC ATCAGCTTAC TCTTCCTTCT CTTCCTCTTC 
ATCGTTGTCT TTGCTCTCCT GGGAATGCAG CTCTTTGGAG GCAGGTTTAA CTTTAATGAT 
GGGACTCCTT CAGCTAATTT TGACACCTTC CCTGCAGCTA TCATGACAGT GTTCCAGATC 
CTGACAGGGG AAGACTGGAA TGAGGTGATG TACAATGGCA TTCGCTCCCA GGGTGGGGTC 
AGCTCAGGCA TGTGGTCTGC CATCTACTTC ATTGTGCTCA CCTTGTTTGG AAACTACACG 
CTGCTAAATG TATTCTTGGC CATCGCTGTG GACAATCTGG CCAATGCCCA GGAACTGACC 
AAGGATGAGC AGGAGGAAGA AGAAGCCTTC AACCAGAAAC ATGCACTGCA GAAGGCCAAG 
GAGGTCAGCC CGATGTCTGC TCCCAACATG CCTTCCATTG AAAGAGACAG AAGGAGAAGA 
CACCACATGT CGATGTGGGA GCCACGCAGC AGCCACCTGA GGGAGCGGAG GCGCCGGCAC 
CACATGTCTG TGTGGGAACA ACGCACCAGC CAGCTGAGGA GGCACATGCA AATGTCTAGC 
CAAGAAGCCC TCAACAAAGA GGAGGCCCCG CCAATGAACC CCCTCAATCC ACTCAACCCA 
CTGAGTCCTC TCAACCCCCT CAATGCTCAC CCCAGCCTTT ATCGGAGGCC CAGGCCCATT 
GAGGGCCTAG CCCTAGGTCT GGGTCTTGAA AAGTGTGAAG AAGAACGCAT CAGTCGTGGG 
GGCTCCCTCA AGGGGGACAT AGGGGGCCTG ACTAGCGCCC TGGACAACCA GAGGAGCCCC 
TTGTCCTTGG GCAAACGGGA GCCACCGTGG CTGCCTAGAT CCTGTCATGG AAACTGTGAC 
CCAATCCAGC AGGAGGCTGG GGGAGGAGAG ACTGTGGTGA CTTTTGAGGA CCGGGCCAGG 
CACAGGCAGA GTCAGAGGCG AAGCCGGCAT CGCCGAGTCA GGACTGAGGG CAAGGACTCT 
GCCTCTGCAT CCCGAAGCAG GTCTGCCAGC CAGGAGCGGA GTCTGGATGA AGGGGTGTCC 
GTTGAGGGGG AGAAGGAACA TGAGCCTCAC AGCAGCCACA GGAGCAAAGA GCCGACCATC 
CACGAAGAGG AGAGAACCCA GGACTTAAGG AGGACCAACA GTCTGATGGT CCCCAGGGGC 
TCTGGACTGG TAGGGGCTCT TGATGAAGCA GAAACACCTC TAGTCCAGCC CCAACCTGAG 
CTGGAAGTGG GGAAGGATGC AGCTCTGACA GAGCAGGAGG CTGAAGGCAG CAGTGAACAA 
GCCCTTCTTG GGGACGTACA GCTGGATGTG GGCCGGGGCA TTAGCCAGAG TGAACCGGAT 
CTCTCCTGCA TGACAGCCAA CATGGACAAG GCTACCACGG AGAGCACCAG TGTCACGGTC 
GCCATCCCTG ATGTGGACCC CTTGGTGGAC TCGACAGTGG TGAACATTAG CAACAAGACG 
GATGGAGAAG CCAGTCCCTT GAAGGAGGCA GAGACTAAAG AGGAAGAAGA AGAGGTAGAG 
AAGAAGAAGA AGCAGAAGAA GGAGAAGCGA GAGACTGGAA AAGCCATGGT GCCCCATAGC 
TCTATGTTCA TCTTCAGCAC CACTAACCCG ATCCGCAGGG CCTGCCACTA CATTGTGAAC 
CTACGCTACT TTGAAATGTG CATTCTCTTG GTGATTGCGG CCAGCAGCAT TGCCCTGGCA 
GCCGAGGACC CTGTACTAAC TAACTCGGAG CGTAACAAAG TCCTGAGATA TTTCGACTAT 
GTTTTCACAG GAGTGTTTAC CTTTGAGATG GTTATAAAGA TGATAGACCA AGGCTTGATC 
CTGCAGGATG GATCATACTT CAGAGACCTC TGGAACATCC TGGACTTTGT AGTAGTGGTT 
GGTGCATTGG TAGCATTTGC TCTGGCGAAC GCTTTGGGAA CCAACAAGGG GAGGGATATC 
AAGACTATCA AGTCTCTGAG GGTACTTCGA GTTCTGCGGC CACTGAAAAC CATCAAGCGC 
TTGCCCAAGC TGAAGGCTGT GTTTGACTGC GTGGTGACCT CCTTGAAGAA TGTCTTCAAC 
ATACTCATTG TCTACAAGCT CTTCATGTTC ATCTTTGCCG TCATCGCTGT TCAGCTATTC 
AAAGGAAAGT TCTTTTATTG CACTGACAGT TCCAAGGACA CAGAGAAGGA GTGCATAGGC 
AACTATGTAG ACCACGAGAA AAACAAGATG GAGGTGAAGG GCCGGGAATG GAAGCGTCAT 
GAATTCCACT ATGACAACAT CATCTGGGCC CTGCTTACCC TCTTCACAGT CTCTACAGGG 
GAAGGATGGC CTCAGGTTCT GCAGCACTCT GTAGATGTGA CAGAGGAGGA CAGAGGCCCA 
AGTCGCAGCA ACCGCATGGA GATGTCCATC TTTTATGTGG TCTACTTTGT GGTCTTCCCT 
TTCTTCTTTG TCAATATCTT CGTGGCTCTC ATTATCATCA CCTTCCAGGA GCAAGGGGAC 
AAGATGATGG AGGAGTGTAG CCTGGAGAAG AATGAGCGGG CATGCATCGA CTTCGCCATT 
AGTGCCAAAC CTCTCACCCG CTACATGCCA CAGAACAGGC ACACCTTCCA GTACCGTGTG 
TGGCACTTTG TAGTCTCCCC ATCCTTTGAG TATACCATCA TGGCCATGAT TGCCTTGAAC 
ACTGTCGTGC TGATGATGAA GTACTACTCT GCTCCTTGCA CCTATGAGCT CGCCTTAAAG 
TACCTGAACA TCGCCTTCAC TATGGTGTTT TCCCTGGAGT GTGTCCTAAA GGTCATTGCT 
TTCGGCTTCC TGAACTATTT CCGAGATACC TGGAACATCT TTGACTTCAT CACGGTGATC 
GGCAGTATCA CAGAAATTAT CCTGACAGAT AGCAAGCTGG TGAACACCAG TGGCTTCAAT 
ATGAGCTTTC TGAAGCTCTT CCGAGCTGCA CGGCTCATAA AGCTTCTGAG ACAGGGCTAC 
ACCATCCGCA TCTTACTGTG GACCTTCGTG CAGTCCTTTA AGGCTCTTCC CTACGTCTGC 
CTTCTCATTG CCATGCTTTT CTTCATCTAC GCCATCATTG GGATGCAGGT GTTTGGAAAC 
ATAAAGTTAG ATGAGGAGAG TCACATCAAC CGGCACAACA ACTTCCGGAG TTTCTTTGGA 
TCCCTCATGC TGCTTTTCAG GAGTGCCACG GGGGAGGCTT GGCAGGAGAT TATGCTGTCC 
TGTCTCGGTG AGAAGGGTTG TGAGCCTGAC ACCACTGCAC CCTCAGGGCA GAATGAGAGT 
GAGCGCTGTG GCACTGACCT GGCCTACGTT TATTTTGTCT CCTTCATCTT CTTCTGCTCC 
TTCCTGATGC TCAACCTGTT CGTGGCTGTC ATCATGGACA ACTTTGAGTA CCTGACTCGA 
GATTCCTCCA TCCTGGGGCC TCACCACTTA GATGAGTTTG TCCGTGTCTG GGCAGAATAT 
GACAGAGCAG CATGTGGCCG CATCCATTAC ACTGAGATGT ATGAAATGCT GACTCTCATG 
TCACCACCGC TAGGCCTCGG CAAGAGATGT CCCTCCAAAG TGGCCTATAA GAGGCTGGTC 
CTGATGAACA TGCCAGTGGC TGAGGACATG ACAGTCCACT TCACCTCCAC ACTTATGGCT 
CTGATCCGGA CAGCTCTGGA CATTAAAATC GCCAAAGGTG GTGCAGACAG ACAGCAGCTA 
GACTCGGAGC TGCAAAAAGA GACCCTGGCC ATTTGGCCTC ACCTGTCCCA AAAGATGTTG 
GATCTGCTTG TGCCCATGCC CAAAGCCTCT GACCTGACTG TGGGCAAGAT CTATGCAGCA 
ATGATGATCA TGGACTACTA TAAACAGAGT AAGGTAAAGA AACAGAGACA GCAGCTGGAA 
GAACAGAAAA ATGCACCCAT GTTCCAGCGC ATGGAGCCCT CATCACTGCC TCAGGAGATC 
ATTGCTAATG CCAAAGCCCT GCCTTATCTC CAGCAGGACC CTGTTTCTGG CCTGAGTGGT 
CGGAGTGGAT ACCCTTCAAT GAGTCCACTC TCCCCTCAAG AAATATTCCA GTTGGCTTGT 
ATGGACCCAG CGGATGATGG ACAATTCCAG GAACAACAAT CTCTGGTGAA TGCACAAGAG 
CCAGAGGTTA GTGAATTAAA AAGTGTGCAG TCCTCTAACC ATGGCATCTA CCTTCCTCCG 
GACACCCAGG AGCATGCGGG ATCTGGGAGG GCATCCTCTA TGCCACGTCT GACTATGGAT 
CCCCAGGTGG TAACAGACCC TAGCTCTATG CGACGTTCAT TTTCTACAAT TCGGGATAAG 
CGTTCAAATT CCTCCTGGTT GGAGGAGTTC TCCATGGAAC GCAGCAGTGA AAACACTTAC 
AAGTCGCGGC GCAGAAGTTA CCATTCCTCC CTGAGGCTGT CAGCACACCG CCTGAATTCT 
GACTCTGGTC ACAAGTCTGA CACTCACCGA TCAGGAGGCA GGGAGAGAGG CCGATCCAAA 
GAACGAAAGC ACCTCCTCTC TCCAGATGTC TCCCGCTGCA ATTCTGAAGA GCGAGGGACC 
CAGGCCGACT GGGAGTCCCC AGAACGTCGC CAGTCCAGGT CACCCAGTGA AGGAAGGTCA 
CAGACCCCCA ATAGACAGGG TACCGGTTCC CTAAGTGAGA GCTCCATCCC CTCTATTTCC 
GACACCAGCA CCCCAAGACG AAGCCGTCGC CAGCTCCCAC CTGTGCCACC AAAGCCTCGT 
CCCCTCCTCT CCTACAGCTC CCTGATGAGA CACACTGGCG GCATCTCTCC ACCTCCTGAT 
GGAAGTGAGG GTGGATCCCC ACTGGCCTCT CAAGCTCTGG AGAGCAACAG CGCTTGCCTG 
ACCGAGTCTT CCAACTCCTT GCACCCCCAG CAGGGCCAGC ATCCTTCCCC ACAGCACTAC 
ATCTCCGAGC CCTATCTGGC CCTCCATGAA GACTCCCACG CCTCAGACTG TGGCGAGGAG 
GAGACACTCA CCTTTGAGGC AGCCGTAGCC ACTAGCTTGG GCCGGTCCAA TACCATTGGC 
TCTGCGCCAC CCCTACGGCA CAGCTGGCAG ATGCCTAATG GGCACTATCG GCGGCGGAGG 
CGTGGGGGGC CTGGGCCTGG CATGATGTGT GGAGCTGTCA GTGACCTCCT GAGTGACACG 
GAAGAAGACG ATAAGTGCTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011246211.1
CDS	538..7938
Translation	MVPEMRGLSPGRRLPEPTLCPATGGSPEPGAAGRRRHVLALPDEEAWRPQQCALLEANASDEVGMLPASPRSAVAGFDNFFPVQEGEGPGWEGSTTLDAGSSPFLPVSPEVMKRRRGGLIEQRDIIKAHEAHKMQSTPQARRKEWEMARFGEAVVVGRPGSGDGDSDQSRNRQGTPVPASGPAAAYKQSKAQRARTMALYNPIPVRQNCFTVNRSLFIFGEDNIVRKYAKKLIDWPPFEYMILATIIANCIVLALEQHLPEDDKTPMSRRLEKTEPYFIGIFCFEAGIKIVALGFIFHKGSYLRNGWNVMDFIVVLSGILATAGTHFNTHVDLRTLRAVRVLRPLKLVSGIPSLQIVLKSIMKAMVPLLQIGLLLFFAILMFAIIGLEFYSGKLHRACFMNNSGILEGFDPPHPCGVQGCPAGYECKDWIGPNDGITQFDNILFAVLTVFQCITMEGWTTVLYNTNDALGATWNWLYFIPLIIIGSFFVLNLVLGVLSGEFAKERERVENRRAFMKLRRQQQIERELNGYRAWIDKAEEVMLAEENKNSGTSALEVLRRATIKRSRTEAMTRDSSDEHCVDISSVGTPLARASIKSTKVDGASYFRHKERLLRISIRHMVKSQVFYWIVLSVVALNTACVAIVHHNQPQWLTHLLYYAEFLFLGLFLLEMSLKMYGMGPRLYFHSSFNCFDFGVTVGSIFEVVWAIFRPGTSFGISVLRALRLLRIFKITKYWASLRNLVVSLMSSMKSIISLLFLLFLFIVVFALLGMQLFGGRFNFNDGTPSANFDTFPAAIMTVFQILTGEDWNEVMYNGIRSQGGVSSGMWSAIYFIVLTLFGNYTLLNVFLAIAVDNLANAQELTKDEQEEEEAFNQKHALQKAKEVSPMSAPNMPSIERDRRRRHHMSMWEPRSSHLRERRRRHHMSVWEQRTSQLRRHMQMSSQEALNKEEAPPMNPLNPLNPLSPLNPLNAHPSLYRRPRPIEGLALGLGLEKCEEERISRGGSLKGDIGGLTSALDNQRSPLSLGKREPPWLPRSCHGNCDPIQQEAGGGETVVTFEDRARHRQSQRRSRHRRVRTEGKDSASASRSRSASQERSLDEGVSVEGEKEHEPHSSHRSKEPTIHEEERTQDLRRTNSLMVPRGSGLVGALDEAETPLVQPQPELEVGKDAALTEQEAEGSSEQALLGDVQLDVGRGISQSEPDLSCMTANMDKATTESTSVTVAIPDVDPLVDSTVVNISNKTDGEASPLKEAETKEEEEEVEKKKKQKKEKRETGKAMVPHSSMFIFSTTNPIRRACHYIVNLRYFEMCILLVIAASSIALAAEDPVLTNSERNKVLRYFDYVFTGVFTFEMVIKMIDQGLILQDGSYFRDLWNILDFVVVVGALVAFALANALGTNKGRDIKTIKSLRVLRVLRPLKTIKRLPKLKAVFDCVVTSLKNVFNILIVYKLFMFIFAVIAVQLFKGKFFYCTDSSKDTEKECIGNYVDHEKNKMEVKGREWKRHEFHYDNIIWALLTLFTVSTGEGWPQVLQHSVDVTEEDRGPSRSNRMEMSIFYVVYFVVFPFFFVNIFVALIIITFQEQGDKMMEECSLEKNERACIDFAISAKPLTRYMPQNRHTFQYRVWHFVVSPSFEYTIMAMIALNTVVLMMKYYSAPCTYELALKYLNIAFTMVFSLECVLKVIAFGFLNYFRDTWNIFDFITVIGSITEIILTDSKLVNTSGFNMSFLKLFRAARLIKLLRQGYTIRILLWTFVQSFKALPYVCLLIAMLFFIYAIIGMQVFGNIKLDEESHINRHNNFRSFFGSLMLLFRSATGEAWQEIMLSCLGEKGCEPDTTAPSGQNESERCGTDLAYVYFVSFIFFCSFLMLNLFVAVIMDNFEYLTRDSSILGPHHLDEFVRVWAEYDRAACGRIHYTEMYEMLTLMSPPLGLGKRCPSKVAYKRLVLMNMPVAEDMTVHFTSTLMALIRTALDIKIAKGGADRQQLDSELQKETLAIWPHLSQKMLDLLVPMPKASDLTVGKIYAAMMIMDYYKQSKVKKQRQQLEEQKNAPMFQRMEPSSLPQEIIANAKALPYLQQDPVSGLSGRSGYPSMSPLSPQEIFQLACMDPADDGQFQEQQSLVNAQEPEVSELKSVQSSNHGIYLPPDTQEHAGSGRASSMPRLTMDPQVVTDPSSMRRSFSTIRDKRSNSSWLEEFSMERSSENTYKSRRRSYHSSLRLSAHRLNSDSGHKSDTHRSGGRERGRSKERKHLLSPDVSRCNSEERGTQADWESPERRQSRSPSEGRSQTPNRQGTGSLSESSIPSISDTSTPRRSRRQLPPVPPKPRPLLSYSSLMRHTGGISPPPDGSEGGSPLASQALESNSACLTESSNSLHPQQGQHPSPQHYISEPYLALHEDSHASDCGEEETLTFEAAVATSLGRSNTIGSAPPLRHSWQMPNGHYRRRRRGGPGPGMMCGAVSDLLSDTEEDDKC

Target ORF information:

RefSeq Version	XM_011247909.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus calcium channel, voltage-dependent, R type, alpha 1E subunit (Cacna1e), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011247909.2

ORF Insert Sequence:

ATGGTGCCCG AGATGCGTGG ACTGAGCCCA GGCCGAAGGC TGCCCGAGCC CACCCTGTGC 
CCGGCTACAG GAGGGAGCCC TGAGCCCGGG GCTGCTGGCA GGCGCCGCCA CGTCCTGGCT 
CTGCCTGATG AGGAGGCATG GCGGCCGCAG CAGTGCGCCC TGCTCGAGGC CAATGCCTCC 
GACGAGGTGG GCATGTTGCC TGCTTCCCCG CGCTCCGCGG TGGCCGGCTT CGACAACTTC 
TTCCCGGTGC AGGAAGGCGA GGGCCCGGGC TGGGAGGGCA GCACCACGCT GGATGCAGGC 
TCCAGCCCCT TCCTTCCTGT GAGCCCAGAG GTCATGAAGC GGAGGCGAGG GGGGCTCATC 
GAGCAGCGGG ACATCATCAA GGCCCACGAG GCACACAAGA TGCAGAGCAC CCCGCAGGCC 
CGGCGCAAGG AGTGGGAGAT GGCTCGCTTC GGGGAGGCGG TGGTCGTTGG CAGGCCAGGC 
TCAGGCGATG GAGACTCGGA CCAGAGCAGG AACCGACAAG GAACCCCCGT CCCGGCCTCG 
GGGCCGGCGG CCGCCTACAA GCAGTCAAAA GCGCAGAGGG CGCGGACTAT GGCTTTGTAC 
AACCCCATAC CAGTCCGGCA GAACTGTTTC ACGGTCAACA GATCCCTGTT CATCTTCGGA 
GAAGATAACA TTGTCAGGAA ATACGCCAAG AAGCTCATCG ATTGGCCGCC ATTTGAGTAC 
ATGATCCTGG CCACCATCAT TGCCAACTGC ATCGTCTTGG CCCTGGAGCA GCATCTTCCT 
GAGGATGACA AGACCCCAAT GTCTCGAAGA CTGGAGAAGA CAGAACCATA TTTCATTGGG 
ATCTTCTGCT TTGAAGCTGG GATCAAAATT GTGGCTCTAG GGTTCATCTT CCATAAGGGT 
TCATACCTCC GTAATGGCTG GAATGTCATG GACTTCATCG TGGTCCTTAG TGGCATCCTG 
GCCACTGCGG GAACCCACTT CAACACCCAC GTGGACCTGC GGACCCTCCG GGCTGTGCGT 
GTCCTGAGGC CTTTAAAGCT GGTATCAGGA ATACCTAGCC TGCAGATCGT GCTGAAGTCT 
ATCATGAAGG CCATGGTGCC TCTTCTGCAG ATTGGCCTTC TGCTCTTCTT TGCCATCCTC 
ATGTTTGCTA TCATTGGCTT GGAGTTCTAC AGTGGCAAGT TGCATCGAGC ATGCTTCATG 
AACAATTCAG GTATTCTAGA GGGGTTTGAT CCTCCTCACC CGTGTGGTGT GCAAGGCTGC 
CCGGCTGGTT ATGAATGTAA GGACTGGATC GGCCCCAATG ACGGGATCAC CCAGTTTGAC 
AACATCCTTT TTGCCGTGCT GACTGTCTTC CAATGCATCA CTATGGAAGG GTGGACCACT 
GTGCTGTACA ATACCAATGA TGCCTTAGGA GCCACCTGGA ACTGGCTGTA CTTCATCCCT 
CTCATCATCA TCGGATCCTT CTTTGTTCTC AACCTCGTCC TGGGAGTGCT TTCCGGGGAA 
TTTGCCAAAG AAAGAGAGAG AGTGGAGAAC CGAAGAGCGT TCATGAAGCT CCGGCGCCAG 
CAACAGATTG AGCGAGAATT GAACGGCTAC CGTGCCTGGA TAGACAAAGC AGAGGAAGTC 
ATGCTTGCTG AAGAAAATAA AAACTCGGGA ACATCAGCCT TGGAAGTGCT TCGAAGGGCA 
ACCATCAAAA GGAGCCGGAC GGAGGCCATG ACCCGAGACT CCAGCGATGA GCACTGCGTT 
GATATCTCTT CAGTGGGCAC ACCTCTTGCC AGAGCCAGTA TCAAGAGCAC AAAAGTAGAT 
GGGGCCTCCT ATTTCCGGCA CAAGGAACGG CTTCTGCGCA TCTCTATCCG CCACATGGTC 
AAGTCCCAAG TCTTTTACTG GATCGTCCTG AGTGTTGTGG CTCTTAATAC TGCCTGTGTG 
GCCATTGTTC ATCACAACCA GCCACAGTGG CTCACTCACC TCCTCTACTA TGCAGAGTTT 
TTGTTTCTGG GACTCTTCCT GTTGGAGATG TCCCTGAAGA TGTATGGCAT GGGGCCACGC 
CTCTATTTTC ACTCATCCTT CAACTGCTTT GATTTTGGGG TCACAGTGGG CAGCATCTTT 
GAGGTTGTCT GGGCAATCTT CAGACCTGGC ACATCTTTTG GAATCAGTGT CTTACGAGCT 
CTCCGGCTCC TAAGGATATT TAAAATAACC AAGTATTGGG CATCCCTCCG GAATCTGGTT 
GTCTCCTTGA TGAGCTCCAT GAAGTCTATC ATCAGCTTAC TCTTCCTTCT CTTCCTCTTC 
ATCGTTGTCT TTGCTCTCCT GGGAATGCAG CTCTTTGGAG GCAGGTTTAA CTTTAATGAT 
GGGACTCCTT CAGCTAATTT TGACACCTTC CCTGCAGCTA TCATGACAGT GTTCCAGATC 
CTGACAGGGG AAGACTGGAA TGAGGTGATG TACAATGGCA TTCGCTCCCA GGGTGGGGTC 
AGCTCAGGCA TGTGGTCTGC CATCTACTTC ATTGTGCTCA CCTTGTTTGG AAACTACACG 
CTGCTAAATG TATTCTTGGC CATCGCTGTG GACAATCTGG CCAATGCCCA GGAACTGACC 
AAGGATGAGC AGGAGGAAGA AGAAGCCTTC AACCAGAAAC ATGCACTGCA GAAGGCCAAG 
GAGGTCAGCC CGATGTCTGC TCCCAACATG CCTTCCATTG AAAGAGACAG AAGGAGAAGA 
CACCACATGT CGATGTGGGA GCCACGCAGC AGCCACCTGA GGGAGCGGAG GCGCCGGCAC 
CACATGTCTG TGTGGGAACA ACGCACCAGC CAGCTGAGGA GGCACATGCA AATGTCTAGC 
CAAGAAGCCC TCAACAAAGA GGAGGCCCCG CCAATGAACC CCCTCAATCC ACTCAACCCA 
CTGAGTCCTC TCAACCCCCT CAATGCTCAC CCCAGCCTTT ATCGGAGGCC CAGGCCCATT 
GAGGGCCTAG CCCTAGGTCT GGGTCTTGAA AAGTGTGAAG AAGAACGCAT CAGTCGTGGG 
GGCTCCCTCA AGGGGGACAT AGGGGGCCTG ACTAGCGCCC TGGACAACCA GAGGAGCCCC 
TTGTCCTTGG GCAAACGGGA GCCACCGTGG CTGCCTAGAT CCTGTCATGG AAACTGTGAC 
CCAATCCAGC AGGAGGCTGG GGGAGGAGAG ACTGTGGTGA CTTTTGAGGA CCGGGCCAGG 
CACAGGCAGA GTCAGAGGCG AAGCCGGCAT CGCCGAGTCA GGACTGAGGG CAAGGACTCT 
GCCTCTGCAT CCCGAAGCAG GTCTGCCAGC CAGGAGCGGA GTCTGGATGA AGGGGTGTCC 
GTTGAGGGGG AGAAGGAACA TGAGCCTCAC AGCAGCCACA GGAGCAAAGA GCCGACCATC 
CACGAAGAGG AGAGAACCCA GGACTTAAGG AGGACCAACA GTCTGATGGT CCCCAGGGGC 
TCTGGACTGG TAGGGGCTCT TGATGAAGCA GAAACACCTC TAGTCCAGCC CCAACCTGAG 
CTGGAAGTGG GGAAGGATGC AGCTCTGACA GAGCAGGAGG CTGAAGGCAG CAGTGAACAA 
GCCCTTCTTG GGGACGTACA GCTGGATGTG GGCCGGGGCA TTAGCCAGAG TGAACCGGAT 
CTCTCCTGCA TGACAGCCAA CATGGACAAG GCTACCACGG AGAGCACCAG TGTCACGGTC 
GCCATCCCTG ATGTGGACCC CTTGGTGGAC TCGACAGTGG TGAACATTAG CAACAAGACG 
GATGGAGAAG CCAGTCCCTT GAAGGAGGCA GAGACTAAAG AGGAAGAAGA AGAGGTAGAG 
AAGAAGAAGA AGCAGAAGAA GGAGAAGCGA GAGACTGGAA AAGCCATGGT GCCCCATAGC 
TCTATGTTCA TCTTCAGCAC CACTAACCCG ATCCGCAGGG CCTGCCACTA CATTGTGAAC 
CTACGCTACT TTGAAATGTG CATTCTCTTG GTGATTGCGG CCAGCAGCAT TGCCCTGGCA 
GCCGAGGACC CTGTACTAAC TAACTCGGAG CGTAACAAAG TCCTGAGATA TTTCGACTAT 
GTTTTCACAG GAGTGTTTAC CTTTGAGATG GTTATAAAGA TGATAGACCA AGGCTTGATC 
CTGCAGGATG GATCATACTT CAGAGACCTC TGGAACATCC TGGACTTTGT AGTAGTGGTT 
GGTGCATTGG TAGCATTTGC TCTGGCGAAC GCTTTGGGAA CCAACAAGGG GAGGGATATC 
AAGACTATCA AGTCTCTGAG GGTACTTCGA GTTCTGCGGC CACTGAAAAC CATCAAGCGC 
TTGCCCAAGC TGAAGGCTGT GTTTGACTGC GTGGTGACCT CCTTGAAGAA TGTCTTCAAC 
ATACTCATTG TCTACAAGCT CTTCATGTTC ATCTTTGCCG TCATCGCTGT TCAGCTATTC 
AAAGGAAAGT TCTTTTATTG CACTGACAGT TCCAAGGACA CAGAGAAGGA GTGCATAGGC 
AACTATGTAG ACCACGAGAA AAACAAGATG GAGGTGAAGG GCCGGGAATG GAAGCGTCAT 
GAATTCCACT ATGACAACAT CATCTGGGCC CTGCTTACCC TCTTCACAGT CTCTACAGGG 
GAAGGATGGC CTCAGGTTCT GCAGCACTCT GTAGATGTGA CAGAGGAGGA CAGAGGCCCA 
AGTCGCAGCA ACCGCATGGA GATGTCCATC TTTTATGTGG TCTACTTTGT GGTCTTCCCT 
TTCTTCTTTG TCAATATCTT CGTGGCTCTC ATTATCATCA CCTTCCAGGA GCAAGGGGAC 
AAGATGATGG AGGAGTGTAG CCTGGAGAAG AATGAGCGGG CATGCATCGA CTTCGCCATT 
AGTGCCAAAC CTCTCACCCG CTACATGCCA CAGAACAGGC ACACCTTCCA GTACCGTGTG 
TGGCACTTTG TAGTCTCCCC ATCCTTTGAG TATACCATCA TGGCCATGAT TGCCTTGAAC 
ACTGTCGTGC TGATGATGAA GTACTACTCT GCTCCTTGCA CCTATGAGCT CGCCTTAAAG 
TACCTGAACA TCGCCTTCAC TATGGTGTTT TCCCTGGAGT GTGTCCTAAA GGTCATTGCT 
TTCGGCTTCC TGAACTATTT CCGAGATACC TGGAACATCT TTGACTTCAT CACGGTGATC 
GGCAGTATCA CAGAAATTAT CCTGACAGAT AGCAAGCTGG TGAACACCAG TGGCTTCAAT 
ATGAGCTTTC TGAAGCTCTT CCGAGCTGCA CGGCTCATAA AGCTTCTGAG ACAGGGCTAC 
ACCATCCGCA TCTTACTGTG GACCTTCGTG CAGTCCTTTA AGGCTCTTCC CTACGTCTGC 
CTTCTCATTG CCATGCTTTT CTTCATCTAC GCCATCATTG GGATGCAGGT GTTTGGAAAC 
ATAAAGTTAG ATGAGGAGAG TCACATCAAC CGGCACAACA ACTTCCGGAG TTTCTTTGGA 
TCCCTCATGC TGCTTTTCAG GAGTGCCACG GGGGAGGCTT GGCAGGAGAT TATGCTGTCC 
TGTCTCGGTG AGAAGGGTTG TGAGCCTGAC ACCACTGCAC CCTCAGGGCA GAATGAGAGT 
GAGCGCTGTG GCACTGACCT GGCCTACGTT TATTTTGTCT CCTTCATCTT CTTCTGCTCC 
TTCCTGATGC TCAACCTGTT CGTGGCTGTC ATCATGGACA ACTTTGAGTA CCTGACTCGA 
GATTCCTCCA TCCTGGGGCC TCACCACTTA GATGAGTTTG TCCGTGTCTG GGCAGAATAT 
GACAGAGCAG CATGTGGCCG CATCCATTAC ACTGAGATGT ATGAAATGCT GACTCTCATG 
TCACCACCGC TAGGCCTCGG CAAGAGATGT CCCTCCAAAG TGGCCTATAA GAGGCTGGTC 
CTGATGAACA TGCCAGTGGC TGAGGACATG ACAGTCCACT TCACCTCCAC ACTTATGGCT 
CTGATCCGGA CAGCTCTGGA CATTAAAATC GCCAAAGGTG GTGCAGACAG ACAGCAGCTA 
GACTCGGAGC TGCAAAAAGA GACCCTGGCC ATTTGGCCTC ACCTGTCCCA AAAGATGTTG 
GATCTGCTTG TGCCCATGCC CAAAGCCTCT GACCTGACTG TGGGCAAGAT CTATGCAGCA 
ATGATGATCA TGGACTACTA TAAACAGAGT AAGGTAAAGA AACAGAGACA GCAGCTGGAA 
GAACAGAAAA ATGCACCCAT GTTCCAGCGC ATGGAGCCCT CATCACTGCC TCAGGAGATC 
ATTGCTAATG CCAAAGCCCT GCCTTATCTC CAGCAGGACC CTGTTTCTGG CCTGAGTGGT 
CGGAGTGGAT ACCCTTCAAT GAGTCCACTC TCCCCTCAAG AAATATTCCA GTTGGCTTGT 
ATGGACCCAG CGGATGATGG ACAATTCCAG GAACAACAAT CTCTGGTGAA TGCACAAGAG 
CCAGAGGTTA GTGAATTAAA AAGTGTGCAG TCCTCTAACC ATGGCATCTA CCTTCCTCCG 
GACACCCAGG AGCATGCGGG ATCTGGGAGG GCATCCTCTA TGCCACGTCT GACTATGGAT 
CCCCAGGTGG TAACAGACCC TAGCTCTATG CGACGTTCAT TTTCTACAAT TCGGGATAAG 
CGTTCAAATT CCTCCTGGTT GGAGGAGTTC TCCATGGAAC GCAGCAGTGA AAACACTTAC 
AAGTCGCGGC GCAGAAGTTA CCATTCCTCC CTGAGGCTGT CAGCACACCG CCTGAATTCT 
GACTCTGGTC ACAAGTCTGA CACTCACCGA TCAGGAGGCA GGGAGAGAGG CCGATCCAAA 
GAACGAAAGC ACCTCCTCTC TCCAGATGTC TCCCGCTGCA ATTCTGAAGA GCGAGGGACC 
CAGGCCGACT GGGAGTCCCC AGAACGTCGC CAGTCCAGGT CACCCAGTGA AGGAAGGTCA 
CAGACCCCCA ATAGACAGGG TACCGGTTCC CTAAGTGAGA GCTCCATCCC CTCTATTTCC 
GACACCAGCA CCCCAAGACG AAGCCGTCGC CAGCTCCCAC CTGTGCCACC AAAGCCTCGT 
CCCCTCCTCT CCTACAGCTC CCTGATGAGA CACACTGGCG GCATCTCTCC ACCTCCTGAT 
GGAAGTGAGG GTGGATCCCC ACTGGCCTCT CAAGCTCTGG AGAGCAACAG CGCTTGCCTG 
ACCGAGTCTT CCAACTCCTT GCACCCCCAG CAGGGCCAGC ATCCTTCCCC ACAGCACTAC 
ATCTCCGAGC CCTATCTGGC CCTCCATGAA GACTCCCACG CCTCAGACTG TGGCGAGGAG 
GAGACACTCA CCTTTGAGGC AGCCGTAGCC ACTAGCTTGG GCCGGTCCAA TACCATTGGC 
TCTGCGCCAC CCCTACGGCA CAGCTGGCAG ATGCCTAATG GGCACTATCG GCGGCGGAGG 
CGTGGGGGGC CTGGGCCTGG CATGATGTGT GGAGCTGTCA GTGACCTCCT GAGTGACACG 
GAAGAAGACG ATAAGTGCTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cacna1e ( XM_017312613.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017168102.1 Mus musculus calcium channel, voltage-dependent, R type, alpha 1E subunit (Cacna1e), transcript variant X5, mRNA.

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CloneID	OMu67261
Clone ID Related Accession (Same CDS sequence)	XM_017312613.1
Accession Version	XM_017312613.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7401bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	voltage-dependent R-type calcium channel subunit alpha-1E isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGCCCG AGATGCGTGG ACTGAGCCCA GGCCGAAGGC TGCCCGAGCC CACCCTGTGC 
CCGGCTACAG GAGGGAGCCC TGAGCCCGGG GCTGCTGGCA GGCGCCGCCA CGTCCTGGCT 
CTGCCTGATG AGGAGGCATG GCGGCCGCAG CAGTGCGCCC TGCTCGAGGC CAATGCCTCC 
GACGAGGTGG GCATGTTGCC TGCTTCCCCG CGCTCCGCGG TGGCCGGCTT CGACAACTTC 
TTCCCGGTGC AGGAAGGCGA GGGCCCGGGC TGGGAGGGCA GCACCACGCT GGATGCAGGC 
TCCAGCCCCT TCCTTCCTGT GAGCCCAGAG GTCATGAAGC GGAGGCGAGG GGGGCTCATC 
GAGCAGCGGG ACATCATCAA GGCCCACGAG GCACACAAGA TGCAGAGCAC CCCGCAGGCC 
CGGCGCAAGG AGTGGGAGAT GGCTCGCTTC GGGGAGGCGG TGGTCGTTGG CAGGCCAGGC 
TCAGGCGATG GAGACTCGGA CCAGAGCAGG AACCGACAAG GAACCCCCGT CCCGGCCTCG 
GGGCCGGCGG CCGCCTACAA GCAGTCAAAA GCGCAGAGGG CGCGGACTAT GGCTTTGTAC 
AACCCCATAC CAGTCCGGCA GAACTGTTTC ACGGTCAACA GATCCCTGTT CATCTTCGGA 
GAAGATAACA TTGTCAGGAA ATACGCCAAG AAGCTCATCG ATTGGCCGCC ATTTGAGTAC 
ATGATCCTGG CCACCATCAT TGCCAACTGC ATCGTCTTGG CCCTGGAGCA GCATCTTCCT 
GAGGATGACA AGACCCCAAT GTCTCGAAGA CTGGAGAAGA CAGAACCATA TTTCATTGGG 
ATCTTCTGCT TTGAAGCTGG GATCAAAATT GTGGCTCTAG GGTTCATCTT CCATAAGGGT 
TCATACCTCC GTAATGGCTG GAATGTCATG GACTTCATCG TGGTCCTTAG TGGCATCCTG 
GCCACTGCGG GAACCCACTT CAACACCCAC GTGGACCTGC GGACCCTCCG GGCTGTGCGT 
GTCCTGAGGC CTTTAAAGCT GGTATCAGGA ATACCTAGCC TGCAGATCGT GCTGAAGTCT 
ATCATGAAGG CCATGGTGCC TCTTCTGCAG ATTGGCCTTC TGCTCTTCTT TGCCATCCTC 
ATGTTTGCTA TCATTGGCTT GGAGTTCTAC AGTGGCAAGT TGCATCGAGC ATGCTTCATG 
AACAATTCAG GTATTCTAGA GGGGTTTGAT CCTCCTCACC CGTGTGGTGT GCAAGGCTGC 
CCGGCTGGTT ATGAATGTAA GGACTGGATC GGCCCCAATG ACGGGATCAC CCAGTTTGAC 
AACATCCTTT TTGCCGTGCT GACTGTCTTC CAATGCATCA CTATGGAAGG GTGGACCACT 
GTGCTGTACA ATACCAATGA TGCCTTAGGA GCCACCTGGA ACTGGCTGTA CTTCATCCCT 
CTCATCATCA TCGGATCCTT CTTTGTTCTC AACCTCGTCC TGGGAGTGCT TTCCGGGGAA 
TTTGCCAAAG AAAGAGAGAG AGTGGAGAAC CGAAGAGCGT TCATGAAGCT CCGGCGCCAG 
CAACAGATTG AGCGAGAATT GAACGGCTAC CGTGCCTGGA TAGACAAAGC AGAGGAAGTC 
ATGCTTGCTG AAGAAAATAA AAACTCGGGA ACATCAGCCT TGGAAGTGCT TCGAAGGGCA 
ACCATCAAAA GGAGCCGGAC GGAGGCCATG ACCCGAGACT CCAGCGATGA GCACTGCGTT 
GATATCTCTT CAGTGGGCAC ACCTCTTGCC AGAGCCAGTA TCAAGAGCAC AAAAGTAGAT 
GGGGCCTCCT ATTTCCGGCA CAAGGAACGG CTTCTGCGCA TCTCTATCCG CCACATGGTC 
AAGTCCCAAG TCTTTTACTG GATCGTCCTG AGTGTTGTGG CTCTTAATAC TGCCTGTGTG 
GCCATTGTTC ATCACAACCA GCCACAGTGG CTCACTCACC TCCTCTACTA TGCAGAGTTT 
TTGTTTCTGG GACTCTTCCT GTTGGAGATG TCCCTGAAGA TGTATGGCAT GGGGCCACGC 
CTCTATTTTC ACTCATCCTT CAACTGCTTT GATTTTGGGG TCACAGTGGG CAGCATCTTT 
GAGGTTGTCT GGGCAATCTT CAGACCTGGC ACATCTTTTG GAATCAGTGT CTTACGAGCT 
CTCCGGCTCC TAAGGATATT TAAAATAACC AAGTATTGGG CATCCCTCCG GAATCTGGTT 
GTCTCCTTGA TGAGCTCCAT GAAGTCTATC ATCAGCTTAC TCTTCCTTCT CTTCCTCTTC 
ATCGTTGTCT TTGCTCTCCT GGGAATGCAG CTCTTTGGAG GCAGGTTTAA CTTTAATGAT 
GGGACTCCTT CAGCTAATTT TGACACCTTC CCTGCAGCTA TCATGACAGT GTTCCAGATC 
CTGACAGGGG AAGACTGGAA TGAGGTGATG TACAATGGCA TTCGCTCCCA GGGTGGGGTC 
AGCTCAGGCA TGTGGTCTGC CATCTACTTC ATTGTGCTCA CCTTGTTTGG AAACTACACG 
CTGCTAAATG TATTCTTGGC CATCGCTGTG GACAATCTGG CCAATGCCCA GGAACTGACC 
AAGGATGAGC AGGAGGAAGA AGAAGCCTTC AACCAGAAAC ATGCACTGCA GAAGGCCAAG 
GAGGTCAGCC CGATGTCTGC TCCCAACATG CCTTCCATTG AAAGAGACAG AAGGAGAAGA 
CACCACATGT CGATGTGGGA GCCACGCAGC AGCCACCTGA GGGAGCGGAG GCGCCGGCAC 
CACATGTCTG TGTGGGAACA ACGCACCAGC CAGCTGAGGA GGCACATGCA AATGTCTAGC 
CAAGAAGCCC TCAACAAAGA GGAGGCCCCG CCAATGAACC CCCTCAATCC ACTCAACCCA 
CTGAGTCCTC TCAACCCCCT CAATGCTCAC CCCAGCCTTT ATCGGAGGCC CAGGCCCATT 
GAGGGCCTAG CCCTAGGTCT GGGTCTTGAA AAGTGTGAAG AAGAACGCAT CAGTCGTGGG 
GGCTCCCTCA AGGGGGACAT AGGGGGCCTG ACTAGCGCCC TGGACAACCA GAGGAGCCCC 
TTGTCCTTGG GCAAACGGGA GCCACCGTGG CTGCCTAGAT CCTGTCATGG AAACTGTGAC 
CCAATCCAGC AGGAGGCTGG GGGAGGAGAG ACTGTGGTGA CTTTTGAGGA CCGGGCCAGG 
CACAGGCAGA GTCAGAGGCG AAGCCGGCAT CGCCGAGTCA GGACTGAGGG CAAGGACTCT 
GCCTCTGCAT CCCGAAGCAG GTCTGCCAGC CAGGAGCGGA GTCTGGATGA AGGGGTGTCC 
GTTGAGGGGG AGAAGGAACA TGAGCCTCAC AGCAGCCACA GGAGCAAAGA GCCGACCATC 
CACGAAGAGG AGAGAACCCA GGACTTAAGG AGGACCAACA GTCTGATGGT CCCCAGGGGC 
TCTGGACTGG TAGGGGCTCT TGATGAAGCA GAAACACCTC TAGTCCAGCC CCAACCTGAG 
CTGGAAGTGG GGAAGGATGC AGCTCTGACA GAGCAGGAGG CTGAAGGCAG CAGTGAACAA 
GCCCTTCTTG GGGACGTACA GCTGGATGTG GGCCGGGGCA TTAGCCAGAG TGAACCGGAT 
CTCTCCTGCA TGACAGCCAA CATGGACAAG GCTACCACGG AGAGCACCAG TGTCACGGTC 
GCCATCCCTG ATGTGGACCC CTTGGTGGAC TCGACAGTGG TGAACATTAG CAACAAGACG 
GATGGAGAAG CCAGTCCCTT GAAGGAGGCA GAGACTAAAG AGGAAGAAGA AGAGGTAGAG 
AAGAAGAAGA AGCAGAAGAA GGAGAAGCGA GAGACTGGAA AAGCCATGGT GCCCCATAGC 
TCTATGTTCA TCTTCAGCAC CACTAACCCG ATCCGCAGGG CCTGCCACTA CATTGTGAAC 
CTACGCTACT TTGAAATGTG CATTCTCTTG GTGATTGCGG CCAGCAGCAT TGCCCTGGCA 
GCCGAGGACC CTGTACTAAC TAACTCGGAG CGTAACAAAG TCCTGAGATA TTTCGACTAT 
GTTTTCACAG GAGTGTTTAC CTTTGAGATG GTTATAAAGA TGATAGACCA AGGCTTGATC 
CTGCAGGATG GATCATACTT CAGAGACCTC TGGAACATCC TGGACTTTGT AGTAGTGGTT 
GGTGCATTGG TAGCATTTGC TCTGGCGAAC GCTTTGGGAA CCAACAAGGG GAGGGATATC 
AAGACTATCA AGTCTCTGAG GGTACTTCGA GTTCTGCGGC CACTGAAAAC CATCAAGCGC 
TTGCCCAAGC TGAAGGCTGT GTTTGACTGC GTGGTGACCT CCTTGAAGAA TGTCTTCAAC 
ATACTCATTG TCTACAAGCT CTTCATGTTC ATCTTTGCCG TCATCGCTGT TCAGCTATTC 
AAAGGAAAGT TCTTTTATTG CACTGACAGT TCCAAGGACA CAGAGAAGGA GTGCATAGGC 
AACTATGTAG ACCACGAGAA AAACAAGATG GAGGTGAAGG GCCGGGAATG GAAGCGTCAT 
GAATTCCACT ATGACAACAT CATCTGGGCC CTGCTTACCC TCTTCACAGT CTCTACAGGG 
GAAGGATGGC CTCAGGTTCT GCAGCACTCT GTAGATGTGA CAGAGGAGGA CAGAGGCCCA 
AGTCGCAGCA ACCGCATGGA GATGTCCATC TTTTATGTGG TCTACTTTGT GGTCTTCCCT 
TTCTTCTTTG TCAATATCTT CGTGGCTCTC ATTATCATCA CCTTCCAGGA GCAAGGGGAC 
AAGATGATGG AGGAGTGTAG CCTGGAGAAG AATGAGCGGG CATGCATCGA CTTCGCCATT 
AGTGCCAAAC CTCTCACCCG CTACATGCCA CAGAACAGGC ACACCTTCCA GTACCGTGTG 
TGGCACTTTG TAGTCTCCCC ATCCTTTGAG TATACCATCA TGGCCATGAT TGCCTTGAAC 
ACTGTCGTGC TGATGATGAA GTACTACTCT GCTCCTTGCA CCTATGAGCT CGCCTTAAAG 
TACCTGAACA TCGCCTTCAC TATGGTGTTT TCCCTGGAGT GTGTCCTAAA GGTCATTGCT 
TTCGGCTTCC TGAACTATTT CCGAGATACC TGGAACATCT TTGACTTCAT CACGGTGATC 
GGCAGTATCA CAGAAATTAT CCTGACAGAT AGCAAGCTGG TGAACACCAG TGGCTTCAAT 
ATGAGCTTTC TGAAGCTCTT CCGAGCTGCA CGGCTCATAA AGCTTCTGAG ACAGGGCTAC 
ACCATCCGCA TCTTACTGTG GACCTTCGTG CAGTCCTTTA AGGCTCTTCC CTACGTCTGC 
CTTCTCATTG CCATGCTTTT CTTCATCTAC GCCATCATTG GGATGCAGGT GTTTGGAAAC 
ATAAAGTTAG ATGAGGAGAG TCACATCAAC CGGCACAACA ACTTCCGGAG TTTCTTTGGA 
TCCCTCATGC TGCTTTTCAG GAGTGCCACG GGGGAGGCTT GGCAGGAGAT TATGCTGTCC 
TGTCTCGGTG AGAAGGGTTG TGAGCCTGAC ACCACTGCAC CCTCAGGGCA GAATGAGAGT 
GAGCGCTGTG GCACTGACCT GGCCTACGTT TATTTTGTCT CCTTCATCTT CTTCTGCTCC 
TTCCTGATGC TCAACCTGTT CGTGGCTGTC ATCATGGACA ACTTTGAGTA CCTGACTCGA 
GATTCCTCCA TCCTGGGGCC TCACCACTTA GATGAGTTTG TCCGTGTCTG GGCAGAATAT 
GACAGAGCAG CATGCGGCAG GATCCCGTAT AAGGATATGT ACAAATTAGT GCGGGTGATC 
TCGCCTCCTC TGGGCCTGGG AGAGAACTGC CCCTACAGGG TGGCTTGCAA GAGGCTGGTC 
CTGATGAACA TGCCAGTGGC TGAGGACATG ACAGTCCACT TCACCTCCAC ACTTATGGCT 
CTGATCCGGA CAGCTCTGGA CATTAAAATC GCCAAAGGTG GTGCAGACAG ACAGCAGCTA 
GACTCGGAGC TGCAAAAAGA GACCCTGGCC ATTTGGCCTC ACCTGTCCCA AAAGATGTTG 
GATCTGCTTG TGCCCATGCC CAAAGCCTCT GACCTGACTG TGGGCAAGAT CTATGCAGCA 
ATGATGATCA TGGACTACTA TAAACAGAGT AAGGTAAAGA AACAGAGACA GCAGCTGGAA 
GAACAGAAAA ATGCACCCAT GTTCCAGCGC ATGGAGCCCT CATCACTGCC TCAGGAGATC 
ATTGCTAATG CCAAAGCCCT GCCTTATCTC CAGCAGGACC CTGTTTCTGG CCTGAGTGGT 
CGGAGTGGAT ACCCTTCAAT GAGTCCACTC TCCCCTCAAG AAATATTCCA GTTGGCTTGT 
ATGGACCCAG CGGATGATGG ACAATTCCAG GAACAACAAT CTCTGGTGAA TGCACAAGAG 
CCAGAGGTTA GTGAATTAAA AAGTGTGCAG TCCTCTAACC ATGGCATCTA CCTTCCTCCG 
GACACCCAGG AGCATGCGGG ATCTGGGAGG GCATCCTCTA TGCCACGTCT GACTATGGAT 
CCCCAGGTGG TAACAGACCC TAGCTCTATG CGACGTTCAT TTTCTACAAT TCGGGATAAG 
CGTTCAAATT CCTCCTGGTT GGAGGAGTTC TCCATGGAAC GCAGCAGTGA AAACACTTAC 
AAGTCGCGGC GCAGAAGTTA CCATTCCTCC CTGAGGCTGT CAGCACACCG CCTGAATTCT 
GACTCTGGTC ACAAGTCTGA CACTCACCGA TCAGGAGGCA GGGAGAGAGG CCGATCCAAA 
GAACGAAAGC ACCTCCTCTC TCCAGATGTC TCCCGCTGCA ATTCTGAAGA GCGAGGGACC 
CAGGCCGACT GGGAGTCCCC AGAACGTCGC CAGTCCAGGT CACCCAGTGA AGGAAGGTCA 
CAGACCCCCA ATAGACAGGG TACCGGTTCC CTAAGTGAGA GCTCCATCCC CTCTATTTCC 
GACACCAGCA CCCCAAGACG AAGCCGTCGC CAGCTCCCAC CTGTGCCACC AAAGCCTCGT 
CCCCTCCTCT CCTACAGCTC CCTGATGAGA CACACTGGCG GCATCTCTCC ACCTCCTGAT 
GGAAGTGAGG GTGGATCCCC ACTGGCCTCT CAAGCTCTGG AGAGCAACAG CGCTTGCCTG 
ACCGAGTCTT CCAACTCCTT GCACCCCCAG CAGGGCCAGC ATCCTTCCCC ACAGCACTAC 
ATCTCCGAGC CCTATCTGGC CCTCCATGAA GACTCCCACG CCTCAGACTG TGGCGAGGAG 
GAGACACTCA CCTTTGAGGC AGCCGTAGCC ACTAGCTTGG GCCGGTCCAA TACCATTGGC 
TCTGCGCCAC CCCTACGGCA CAGCTGGCAG ATGCCTAATG GGCACTATCG GCGGCGGAGG 
CGTGGGGGGC CTGGGCCTGG CATGATGTGT GGAGCTGTCA GTGACCTCCT GAGTGACACG 
GAAGAAGACG ATAAGTGCTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017168102.1
CDS	594..7994
Translation	MVPEMRGLSPGRRLPEPTLCPATGGSPEPGAAGRRRHVLALPDEEAWRPQQCALLEANASDEVGMLPASPRSAVAGFDNFFPVQEGEGPGWEGSTTLDAGSSPFLPVSPEVMKRRRGGLIEQRDIIKAHEAHKMQSTPQARRKEWEMARFGEAVVVGRPGSGDGDSDQSRNRQGTPVPASGPAAAYKQSKAQRARTMALYNPIPVRQNCFTVNRSLFIFGEDNIVRKYAKKLIDWPPFEYMILATIIANCIVLALEQHLPEDDKTPMSRRLEKTEPYFIGIFCFEAGIKIVALGFIFHKGSYLRNGWNVMDFIVVLSGILATAGTHFNTHVDLRTLRAVRVLRPLKLVSGIPSLQIVLKSIMKAMVPLLQIGLLLFFAILMFAIIGLEFYSGKLHRACFMNNSGILEGFDPPHPCGVQGCPAGYECKDWIGPNDGITQFDNILFAVLTVFQCITMEGWTTVLYNTNDALGATWNWLYFIPLIIIGSFFVLNLVLGVLSGEFAKERERVENRRAFMKLRRQQQIERELNGYRAWIDKAEEVMLAEENKNSGTSALEVLRRATIKRSRTEAMTRDSSDEHCVDISSVGTPLARASIKSTKVDGASYFRHKERLLRISIRHMVKSQVFYWIVLSVVALNTACVAIVHHNQPQWLTHLLYYAEFLFLGLFLLEMSLKMYGMGPRLYFHSSFNCFDFGVTVGSIFEVVWAIFRPGTSFGISVLRALRLLRIFKITKYWASLRNLVVSLMSSMKSIISLLFLLFLFIVVFALLGMQLFGGRFNFNDGTPSANFDTFPAAIMTVFQILTGEDWNEVMYNGIRSQGGVSSGMWSAIYFIVLTLFGNYTLLNVFLAIAVDNLANAQELTKDEQEEEEAFNQKHALQKAKEVSPMSAPNMPSIERDRRRRHHMSMWEPRSSHLRERRRRHHMSVWEQRTSQLRRHMQMSSQEALNKEEAPPMNPLNPLNPLSPLNPLNAHPSLYRRPRPIEGLALGLGLEKCEEERISRGGSLKGDIGGLTSALDNQRSPLSLGKREPPWLPRSCHGNCDPIQQEAGGGETVVTFEDRARHRQSQRRSRHRRVRTEGKDSASASRSRSASQERSLDEGVSVEGEKEHEPHSSHRSKEPTIHEEERTQDLRRTNSLMVPRGSGLVGALDEAETPLVQPQPELEVGKDAALTEQEAEGSSEQALLGDVQLDVGRGISQSEPDLSCMTANMDKATTESTSVTVAIPDVDPLVDSTVVNISNKTDGEASPLKEAETKEEEEEVEKKKKQKKEKRETGKAMVPHSSMFIFSTTNPIRRACHYIVNLRYFEMCILLVIAASSIALAAEDPVLTNSERNKVLRYFDYVFTGVFTFEMVIKMIDQGLILQDGSYFRDLWNILDFVVVVGALVAFALANALGTNKGRDIKTIKSLRVLRVLRPLKTIKRLPKLKAVFDCVVTSLKNVFNILIVYKLFMFIFAVIAVQLFKGKFFYCTDSSKDTEKECIGNYVDHEKNKMEVKGREWKRHEFHYDNIIWALLTLFTVSTGEGWPQVLQHSVDVTEEDRGPSRSNRMEMSIFYVVYFVVFPFFFVNIFVALIIITFQEQGDKMMEECSLEKNERACIDFAISAKPLTRYMPQNRHTFQYRVWHFVVSPSFEYTIMAMIALNTVVLMMKYYSAPCTYELALKYLNIAFTMVFSLECVLKVIAFGFLNYFRDTWNIFDFITVIGSITEIILTDSKLVNTSGFNMSFLKLFRAARLIKLLRQGYTIRILLWTFVQSFKALPYVCLLIAMLFFIYAIIGMQVFGNIKLDEESHINRHNNFRSFFGSLMLLFRSATGEAWQEIMLSCLGEKGCEPDTTAPSGQNESERCGTDLAYVYFVSFIFFCSFLMLNLFVAVIMDNFEYLTRDSSILGPHHLDEFVRVWAEYDRAACGRIPYKDMYKLVRVISPPLGLGENCPYRVACKRLVLMNMPVAEDMTVHFTSTLMALIRTALDIKIAKGGADRQQLDSELQKETLAIWPHLSQKMLDLLVPMPKASDLTVGKIYAAMMIMDYYKQSKVKKQRQQLEEQKNAPMFQRMEPSSLPQEIIANAKALPYLQQDPVSGLSGRSGYPSMSPLSPQEIFQLACMDPADDGQFQEQQSLVNAQEPEVSELKSVQSSNHGIYLPPDTQEHAGSGRASSMPRLTMDPQVVTDPSSMRRSFSTIRDKRSNSSWLEEFSMERSSENTYKSRRRSYHSSLRLSAHRLNSDSGHKSDTHRSGGRERGRSKERKHLLSPDVSRCNSEERGTQADWESPERRQSRSPSEGRSQTPNRQGTGSLSESSIPSISDTSTPRRSRRQLPPVPPKPRPLLSYSSLMRHTGGISPPPDGSEGGSPLASQALESNSACLTESSNSLHPQQGQHPSPQHYISEPYLALHEDSHASDCGEEETLTFEAAVATSLGRSNTIGSAPPLRHSWQMPNGHYRRRRRGGPGPGMMCGAVSDLLSDTEEDDKC

Target ORF information:

RefSeq Version	XM_017312613.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus calcium channel, voltage-dependent, R type, alpha 1E subunit (Cacna1e), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017312613.1

ORF Insert Sequence:

ATGGTGCCCG AGATGCGTGG ACTGAGCCCA GGCCGAAGGC TGCCCGAGCC CACCCTGTGC 
CCGGCTACAG GAGGGAGCCC TGAGCCCGGG GCTGCTGGCA GGCGCCGCCA CGTCCTGGCT 
CTGCCTGATG AGGAGGCATG GCGGCCGCAG CAGTGCGCCC TGCTCGAGGC CAATGCCTCC 
GACGAGGTGG GCATGTTGCC TGCTTCCCCG CGCTCCGCGG TGGCCGGCTT CGACAACTTC 
TTCCCGGTGC AGGAAGGCGA GGGCCCGGGC TGGGAGGGCA GCACCACGCT GGATGCAGGC 
TCCAGCCCCT TCCTTCCTGT GAGCCCAGAG GTCATGAAGC GGAGGCGAGG GGGGCTCATC 
GAGCAGCGGG ACATCATCAA GGCCCACGAG GCACACAAGA TGCAGAGCAC CCCGCAGGCC 
CGGCGCAAGG AGTGGGAGAT GGCTCGCTTC GGGGAGGCGG TGGTCGTTGG CAGGCCAGGC 
TCAGGCGATG GAGACTCGGA CCAGAGCAGG AACCGACAAG GAACCCCCGT CCCGGCCTCG 
GGGCCGGCGG CCGCCTACAA GCAGTCAAAA GCGCAGAGGG CGCGGACTAT GGCTTTGTAC 
AACCCCATAC CAGTCCGGCA GAACTGTTTC ACGGTCAACA GATCCCTGTT CATCTTCGGA 
GAAGATAACA TTGTCAGGAA ATACGCCAAG AAGCTCATCG ATTGGCCGCC ATTTGAGTAC 
ATGATCCTGG CCACCATCAT TGCCAACTGC ATCGTCTTGG CCCTGGAGCA GCATCTTCCT 
GAGGATGACA AGACCCCAAT GTCTCGAAGA CTGGAGAAGA CAGAACCATA TTTCATTGGG 
ATCTTCTGCT TTGAAGCTGG GATCAAAATT GTGGCTCTAG GGTTCATCTT CCATAAGGGT 
TCATACCTCC GTAATGGCTG GAATGTCATG GACTTCATCG TGGTCCTTAG TGGCATCCTG 
GCCACTGCGG GAACCCACTT CAACACCCAC GTGGACCTGC GGACCCTCCG GGCTGTGCGT 
GTCCTGAGGC CTTTAAAGCT GGTATCAGGA ATACCTAGCC TGCAGATCGT GCTGAAGTCT 
ATCATGAAGG CCATGGTGCC TCTTCTGCAG ATTGGCCTTC TGCTCTTCTT TGCCATCCTC 
ATGTTTGCTA TCATTGGCTT GGAGTTCTAC AGTGGCAAGT TGCATCGAGC ATGCTTCATG 
AACAATTCAG GTATTCTAGA GGGGTTTGAT CCTCCTCACC CGTGTGGTGT GCAAGGCTGC 
CCGGCTGGTT ATGAATGTAA GGACTGGATC GGCCCCAATG ACGGGATCAC CCAGTTTGAC 
AACATCCTTT TTGCCGTGCT GACTGTCTTC CAATGCATCA CTATGGAAGG GTGGACCACT 
GTGCTGTACA ATACCAATGA TGCCTTAGGA GCCACCTGGA ACTGGCTGTA CTTCATCCCT 
CTCATCATCA TCGGATCCTT CTTTGTTCTC AACCTCGTCC TGGGAGTGCT TTCCGGGGAA 
TTTGCCAAAG AAAGAGAGAG AGTGGAGAAC CGAAGAGCGT TCATGAAGCT CCGGCGCCAG 
CAACAGATTG AGCGAGAATT GAACGGCTAC CGTGCCTGGA TAGACAAAGC AGAGGAAGTC 
ATGCTTGCTG AAGAAAATAA AAACTCGGGA ACATCAGCCT TGGAAGTGCT TCGAAGGGCA 
ACCATCAAAA GGAGCCGGAC GGAGGCCATG ACCCGAGACT CCAGCGATGA GCACTGCGTT 
GATATCTCTT CAGTGGGCAC ACCTCTTGCC AGAGCCAGTA TCAAGAGCAC AAAAGTAGAT 
GGGGCCTCCT ATTTCCGGCA CAAGGAACGG CTTCTGCGCA TCTCTATCCG CCACATGGTC 
AAGTCCCAAG TCTTTTACTG GATCGTCCTG AGTGTTGTGG CTCTTAATAC TGCCTGTGTG 
GCCATTGTTC ATCACAACCA GCCACAGTGG CTCACTCACC TCCTCTACTA TGCAGAGTTT 
TTGTTTCTGG GACTCTTCCT GTTGGAGATG TCCCTGAAGA TGTATGGCAT GGGGCCACGC 
CTCTATTTTC ACTCATCCTT CAACTGCTTT GATTTTGGGG TCACAGTGGG CAGCATCTTT 
GAGGTTGTCT GGGCAATCTT CAGACCTGGC ACATCTTTTG GAATCAGTGT CTTACGAGCT 
CTCCGGCTCC TAAGGATATT TAAAATAACC AAGTATTGGG CATCCCTCCG GAATCTGGTT 
GTCTCCTTGA TGAGCTCCAT GAAGTCTATC ATCAGCTTAC TCTTCCTTCT CTTCCTCTTC 
ATCGTTGTCT TTGCTCTCCT GGGAATGCAG CTCTTTGGAG GCAGGTTTAA CTTTAATGAT 
GGGACTCCTT CAGCTAATTT TGACACCTTC CCTGCAGCTA TCATGACAGT GTTCCAGATC 
CTGACAGGGG AAGACTGGAA TGAGGTGATG TACAATGGCA TTCGCTCCCA GGGTGGGGTC 
AGCTCAGGCA TGTGGTCTGC CATCTACTTC ATTGTGCTCA CCTTGTTTGG AAACTACACG 
CTGCTAAATG TATTCTTGGC CATCGCTGTG GACAATCTGG CCAATGCCCA GGAACTGACC 
AAGGATGAGC AGGAGGAAGA AGAAGCCTTC AACCAGAAAC ATGCACTGCA GAAGGCCAAG 
GAGGTCAGCC CGATGTCTGC TCCCAACATG CCTTCCATTG AAAGAGACAG AAGGAGAAGA 
CACCACATGT CGATGTGGGA GCCACGCAGC AGCCACCTGA GGGAGCGGAG GCGCCGGCAC 
CACATGTCTG TGTGGGAACA ACGCACCAGC CAGCTGAGGA GGCACATGCA AATGTCTAGC 
CAAGAAGCCC TCAACAAAGA GGAGGCCCCG CCAATGAACC CCCTCAATCC ACTCAACCCA 
CTGAGTCCTC TCAACCCCCT CAATGCTCAC CCCAGCCTTT ATCGGAGGCC CAGGCCCATT 
GAGGGCCTAG CCCTAGGTCT GGGTCTTGAA AAGTGTGAAG AAGAACGCAT CAGTCGTGGG 
GGCTCCCTCA AGGGGGACAT AGGGGGCCTG ACTAGCGCCC TGGACAACCA GAGGAGCCCC 
TTGTCCTTGG GCAAACGGGA GCCACCGTGG CTGCCTAGAT CCTGTCATGG AAACTGTGAC 
CCAATCCAGC AGGAGGCTGG GGGAGGAGAG ACTGTGGTGA CTTTTGAGGA CCGGGCCAGG 
CACAGGCAGA GTCAGAGGCG AAGCCGGCAT CGCCGAGTCA GGACTGAGGG CAAGGACTCT 
GCCTCTGCAT CCCGAAGCAG GTCTGCCAGC CAGGAGCGGA GTCTGGATGA AGGGGTGTCC 
GTTGAGGGGG AGAAGGAACA TGAGCCTCAC AGCAGCCACA GGAGCAAAGA GCCGACCATC 
CACGAAGAGG AGAGAACCCA GGACTTAAGG AGGACCAACA GTCTGATGGT CCCCAGGGGC 
TCTGGACTGG TAGGGGCTCT TGATGAAGCA GAAACACCTC TAGTCCAGCC CCAACCTGAG 
CTGGAAGTGG GGAAGGATGC AGCTCTGACA GAGCAGGAGG CTGAAGGCAG CAGTGAACAA 
GCCCTTCTTG GGGACGTACA GCTGGATGTG GGCCGGGGCA TTAGCCAGAG TGAACCGGAT 
CTCTCCTGCA TGACAGCCAA CATGGACAAG GCTACCACGG AGAGCACCAG TGTCACGGTC 
GCCATCCCTG ATGTGGACCC CTTGGTGGAC TCGACAGTGG TGAACATTAG CAACAAGACG 
GATGGAGAAG CCAGTCCCTT GAAGGAGGCA GAGACTAAAG AGGAAGAAGA AGAGGTAGAG 
AAGAAGAAGA AGCAGAAGAA GGAGAAGCGA GAGACTGGAA AAGCCATGGT GCCCCATAGC 
TCTATGTTCA TCTTCAGCAC CACTAACCCG ATCCGCAGGG CCTGCCACTA CATTGTGAAC 
CTACGCTACT TTGAAATGTG CATTCTCTTG GTGATTGCGG CCAGCAGCAT TGCCCTGGCA 
GCCGAGGACC CTGTACTAAC TAACTCGGAG CGTAACAAAG TCCTGAGATA TTTCGACTAT 
GTTTTCACAG GAGTGTTTAC CTTTGAGATG GTTATAAAGA TGATAGACCA AGGCTTGATC 
CTGCAGGATG GATCATACTT CAGAGACCTC TGGAACATCC TGGACTTTGT AGTAGTGGTT 
GGTGCATTGG TAGCATTTGC TCTGGCGAAC GCTTTGGGAA CCAACAAGGG GAGGGATATC 
AAGACTATCA AGTCTCTGAG GGTACTTCGA GTTCTGCGGC CACTGAAAAC CATCAAGCGC 
TTGCCCAAGC TGAAGGCTGT GTTTGACTGC GTGGTGACCT CCTTGAAGAA TGTCTTCAAC 
ATACTCATTG TCTACAAGCT CTTCATGTTC ATCTTTGCCG TCATCGCTGT TCAGCTATTC 
AAAGGAAAGT TCTTTTATTG CACTGACAGT TCCAAGGACA CAGAGAAGGA GTGCATAGGC 
AACTATGTAG ACCACGAGAA AAACAAGATG GAGGTGAAGG GCCGGGAATG GAAGCGTCAT 
GAATTCCACT ATGACAACAT CATCTGGGCC CTGCTTACCC TCTTCACAGT CTCTACAGGG 
GAAGGATGGC CTCAGGTTCT GCAGCACTCT GTAGATGTGA CAGAGGAGGA CAGAGGCCCA 
AGTCGCAGCA ACCGCATGGA GATGTCCATC TTTTATGTGG TCTACTTTGT GGTCTTCCCT 
TTCTTCTTTG TCAATATCTT CGTGGCTCTC ATTATCATCA CCTTCCAGGA GCAAGGGGAC 
AAGATGATGG AGGAGTGTAG CCTGGAGAAG AATGAGCGGG CATGCATCGA CTTCGCCATT 
AGTGCCAAAC CTCTCACCCG CTACATGCCA CAGAACAGGC ACACCTTCCA GTACCGTGTG 
TGGCACTTTG TAGTCTCCCC ATCCTTTGAG TATACCATCA TGGCCATGAT TGCCTTGAAC 
ACTGTCGTGC TGATGATGAA GTACTACTCT GCTCCTTGCA CCTATGAGCT CGCCTTAAAG 
TACCTGAACA TCGCCTTCAC TATGGTGTTT TCCCTGGAGT GTGTCCTAAA GGTCATTGCT 
TTCGGCTTCC TGAACTATTT CCGAGATACC TGGAACATCT TTGACTTCAT CACGGTGATC 
GGCAGTATCA CAGAAATTAT CCTGACAGAT AGCAAGCTGG TGAACACCAG TGGCTTCAAT 
ATGAGCTTTC TGAAGCTCTT CCGAGCTGCA CGGCTCATAA AGCTTCTGAG ACAGGGCTAC 
ACCATCCGCA TCTTACTGTG GACCTTCGTG CAGTCCTTTA AGGCTCTTCC CTACGTCTGC 
CTTCTCATTG CCATGCTTTT CTTCATCTAC GCCATCATTG GGATGCAGGT GTTTGGAAAC 
ATAAAGTTAG ATGAGGAGAG TCACATCAAC CGGCACAACA ACTTCCGGAG TTTCTTTGGA 
TCCCTCATGC TGCTTTTCAG GAGTGCCACG GGGGAGGCTT GGCAGGAGAT TATGCTGTCC 
TGTCTCGGTG AGAAGGGTTG TGAGCCTGAC ACCACTGCAC CCTCAGGGCA GAATGAGAGT 
GAGCGCTGTG GCACTGACCT GGCCTACGTT TATTTTGTCT CCTTCATCTT CTTCTGCTCC 
TTCCTGATGC TCAACCTGTT CGTGGCTGTC ATCATGGACA ACTTTGAGTA CCTGACTCGA 
GATTCCTCCA TCCTGGGGCC TCACCACTTA GATGAGTTTG TCCGTGTCTG GGCAGAATAT 
GACAGAGCAG CATGCGGCAG GATCCCGTAT AAGGATATGT ACAAATTAGT GCGGGTGATC 
TCGCCTCCTC TGGGCCTGGG AGAGAACTGC CCCTACAGGG TGGCTTGCAA GAGGCTGGTC 
CTGATGAACA TGCCAGTGGC TGAGGACATG ACAGTCCACT TCACCTCCAC ACTTATGGCT 
CTGATCCGGA CAGCTCTGGA CATTAAAATC GCCAAAGGTG GTGCAGACAG ACAGCAGCTA 
GACTCGGAGC TGCAAAAAGA GACCCTGGCC ATTTGGCCTC ACCTGTCCCA AAAGATGTTG 
GATCTGCTTG TGCCCATGCC CAAAGCCTCT GACCTGACTG TGGGCAAGAT CTATGCAGCA 
ATGATGATCA TGGACTACTA TAAACAGAGT AAGGTAAAGA AACAGAGACA GCAGCTGGAA 
GAACAGAAAA ATGCACCCAT GTTCCAGCGC ATGGAGCCCT CATCACTGCC TCAGGAGATC 
ATTGCTAATG CCAAAGCCCT GCCTTATCTC CAGCAGGACC CTGTTTCTGG CCTGAGTGGT 
CGGAGTGGAT ACCCTTCAAT GAGTCCACTC TCCCCTCAAG AAATATTCCA GTTGGCTTGT 
ATGGACCCAG CGGATGATGG ACAATTCCAG GAACAACAAT CTCTGGTGAA TGCACAAGAG 
CCAGAGGTTA GTGAATTAAA AAGTGTGCAG TCCTCTAACC ATGGCATCTA CCTTCCTCCG 
GACACCCAGG AGCATGCGGG ATCTGGGAGG GCATCCTCTA TGCCACGTCT GACTATGGAT 
CCCCAGGTGG TAACAGACCC TAGCTCTATG CGACGTTCAT TTTCTACAAT TCGGGATAAG 
CGTTCAAATT CCTCCTGGTT GGAGGAGTTC TCCATGGAAC GCAGCAGTGA AAACACTTAC 
AAGTCGCGGC GCAGAAGTTA CCATTCCTCC CTGAGGCTGT CAGCACACCG CCTGAATTCT 
GACTCTGGTC ACAAGTCTGA CACTCACCGA TCAGGAGGCA GGGAGAGAGG CCGATCCAAA 
GAACGAAAGC ACCTCCTCTC TCCAGATGTC TCCCGCTGCA ATTCTGAAGA GCGAGGGACC 
CAGGCCGACT GGGAGTCCCC AGAACGTCGC CAGTCCAGGT CACCCAGTGA AGGAAGGTCA 
CAGACCCCCA ATAGACAGGG TACCGGTTCC CTAAGTGAGA GCTCCATCCC CTCTATTTCC 
GACACCAGCA CCCCAAGACG AAGCCGTCGC CAGCTCCCAC CTGTGCCACC AAAGCCTCGT 
CCCCTCCTCT CCTACAGCTC CCTGATGAGA CACACTGGCG GCATCTCTCC ACCTCCTGAT 
GGAAGTGAGG GTGGATCCCC ACTGGCCTCT CAAGCTCTGG AGAGCAACAG CGCTTGCCTG 
ACCGAGTCTT CCAACTCCTT GCACCCCCAG CAGGGCCAGC ATCCTTCCCC ACAGCACTAC 
ATCTCCGAGC CCTATCTGGC CCTCCATGAA GACTCCCACG CCTCAGACTG TGGCGAGGAG 
GAGACACTCA CCTTTGAGGC AGCCGTAGCC ACTAGCTTGG GCCGGTCCAA TACCATTGGC 
TCTGCGCCAC CCCTACGGCA CAGCTGGCAG ATGCCTAATG GGCACTATCG GCGGCGGAGG 
CGTGGGGGGC CTGGGCCTGG CATGATGTGT GGAGCTGTCA GTGACCTCCT GAGTGACACG 
GAAGAAGACG ATAAGTGCTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cacna1e ( XM_017312628.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017168117.1 Mus musculus calcium channel, voltage-dependent, R type, alpha 1E subunit (Cacna1e), transcript variant X7, mRNA.

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CloneID	OMu67262
Clone ID Related Accession (Same CDS sequence)	XM_017312628.1
Accession Version	XM_017312628.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7389bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	voltage-dependent R-type calcium channel subunit alpha-1E isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGCCCG AGATGCGTGG ACTGAGCCCA GGCCGAAGGC TGCCCGAGCC CACCCTGTGC 
CCGGCTACAG GAGGGAGCCC TGAGCCCGGG GCTGCTGGCA GGCGCCGCCA CGTCCTGGCT 
CTGCCTGATG AGGAGGCATG GCGGCCGCAG CAGTGCGCCC TGCTCGAGGC CAATGCCTCC 
GACGAGGTGG GCATGTTGCC TGCTTCCCCG CGCTCCGCGG TGGCCGGCTT CGACAACTTC 
TTCCCGGTGC AGGAAGGCGA GGGCCCGGGC TGGGAGGGCA GCACCACGCT GGATGCAGGC 
TCCAGCCCCT TCCTTCCTGT GAGCCCAGAG GTCATGAAGC GGAGGCGAGG GGGGCTCATC 
GAGCAGCGGG ACATCATCAA GGCCCACGAG GCACACAAGA TGCAGAGCAC CCCGCAGGCC 
CGGCGCAAGG AGTGGGAGAT GGCTCGCTTC GGGGAGGCGG TGGTCGTTGG CAGGCCAGGC 
TCAGGCGATG GAGACTCGGA CCAGAGCAGG AACCGACAAG GAACCCCCGT CCCGGCCTCG 
GGGCCGGCGG CCGCCTACAA GCAGTCAAAA GCGCAGAGGG CGCGGACTAT GGCTTTGTAC 
AACCCCATAC CAGTCCGGCA GAACTGTTTC ACGGTCAACA GATCCCTGTT CATCTTCGGA 
GAAGATAACA TTGTCAGGAA ATACGCCAAG AAGCTCATCG ATTGGCCGCC ATTTGAGTAC 
ATGATCCTGG CCACCATCAT TGCCAACTGC ATCGTCTTGG CCCTGGAGCA GCATCTTCCT 
GAGGATGACA AGACCCCAAT GTCTCGAAGA CTGGAGAAGA CAGAACCATA TTTCATTGGG 
ATCTTCTGCT TTGAAGCTGG GATCAAAATT GTGGCTCTAG GGTTCATCTT CCATAAGGGT 
TCATACCTCC GTAATGGCTG GAATGTCATG GACTTCATCG TGGTCCTTAG TGGCATCCTG 
GCCACTGCGG GAACCCACTT CAACACCCAC GTGGACCTGC GGACCCTCCG GGCTGTGCGT 
GTCCTGAGGC CTTTAAAGCT GGTATCAGGA ATACCTAGCC TGCAGATCGT GCTGAAGTCT 
ATCATGAAGG CCATGGTGCC TCTTCTGCAG ATTGGCCTTC TGCTCTTCTT TGCCATCCTC 
ATGTTTGCTA TCATTGGCTT GGAGTTCTAC AGTGGCAAGT TGCATCGAGC ATGCTTCATG 
AACAATTCAG GTATTCTAGA GGGGTTTGAT CCTCCTCACC CGTGTGGTGT GCAAGGCTGC 
CCGGCTGGTT ATGAATGTAA GGACTGGATC GGCCCCAATG ACGGGATCAC CCAGTTTGAC 
AACATCCTTT TTGCCGTGCT GACTGTCTTC CAATGCATCA CTATGGAAGG GTGGACCACT 
GTGCTGTACA ATACCAATGA TGCCTTAGGA GCCACCTGGA ACTGGCTGTA CTTCATCCCT 
CTCATCATCA TCGGATCCTT CTTTGTTCTC AACCTCGTCC TGGGAGTGCT TTCCGGGGAA 
TTTGCCAAAG AAAGAGAGAG AGTGGAGAAC CGAAGAGCGT TCATGAAGCT CCGGCGCCAG 
CAACAGATTG AGCGAGAATT GAACGGCTAC CGTGCCTGGA TAGACAAAGC AGAGGAAGTC 
ATGCTTGCTG AAGAAAATAA AAACTCGGGA ACATCAGCCT TGGAAGTGCT TCGAAGGGCA 
ACCATCAAAA GGAGCCGGAC GGAGGCCATG ACCCGAGACT CCAGCGATGA GCACTGCGTT 
GATATCTCTT CAGTGGGCAC ACCTCTTGCC AGAGCCAGTA TCAAGAGCAC AAAAGTAGAT 
GGGGCCTCCT ATTTCCGGCA CAAGGAACGG CTTCTGCGCA TCTCTATCCG CCACATGGTC 
AAGTCCCAAG TCTTTTACTG GATCGTCCTG AGTGTTGTGG CTCTTAATAC TGCCTGTGTG 
GCCATTGTTC ATCACAACCA GCCACAGTGG CTCACTCACC TCCTCTACTA TGCAGAGTTT 
TTGTTTCTGG GACTCTTCCT GTTGGAGATG TCCCTGAAGA TGTATGGCAT GGGGCCACGC 
CTCTATTTTC ACTCATCCTT CAACTGCTTT GATTTTGGGG TCACAGTGGG CAGCATCTTT 
GAGGTTGTCT GGGCAATCTT CAGACCTGGC ACATCTTTTG GAATCAGTGT CTTACGAGCT 
CTCCGGCTCC TAAGGATATT TAAAATAACC AAGTATTGGG CATCCCTCCG GAATCTGGTT 
GTCTCCTTGA TGAGCTCCAT GAAGTCTATC ATCAGCTTAC TCTTCCTTCT CTTCCTCTTC 
ATCGTTGTCT TTGCTCTCCT GGGAATGCAG CTCTTTGGAG GCAGGTTTAA CTTTAATGAT 
GGGACTCCTT CAGCTAATTT TGACACCTTC CCTGCAGCTA TCATGACAGT GTTCCAGATC 
CTGACAGGGG AAGACTGGAA TGAGGTGATG TACAATGGCA TTCGCTCCCA GGGTGGGGTC 
AGCTCAGGCA TGTGGTCTGC CATCTACTTC ATTGTGCTCA CCTTGTTTGG AAACTACACG 
CTGCTAAATG TATTCTTGGC CATCGCTGTG GACAATCTGG CCAATGCCCA GGAACTGACC 
AAGGATGAGC AGGAGGAAGA AGAAGCCTTC AACCAGAAAC ATGCACTGCA GAAGGCCAAG 
GAGGTCAGCC CGATGTCTGC TCCCAACATG CCTTCCATTG AAAGAGACAG AAGGAGAAGA 
CACCACATGT CGATGTGGGA GCCACGCAGC AGCCACCTGA GGGAGCGGAG GCGCCGGCAC 
CACATGTCTG TGTGGGAACA ACGCACCAGC CAGCTGAGGA GGCACATGCA AATGTCTAGC 
CAAGAAGCCC TCAACAAAGA GGAGGCCCCG CCAATGAACC CCCTCAATCC ACTCAACCCA 
CTGAGTCCTC TCAACCCCCT CAATGCTCAC CCCAGCCTTT ATCGGAGGCC CAGGCCCATT 
GAGGGCCTAG CCCTAGGTCT GGGTCTTGAA AAGTGTGAAG AAGAACGCAT CAGTCGTGGG 
GGCTCCCTCA AGGGGGACAT AGGGGGCCTG ACTAGCGCCC TGGACAACCA GAGGAGCCCC 
TTGTCCTTGG GCAAACGGGA GCCACCGTGG CTGCCTAGAT CCTGTCATGG AAACTGTGAC 
CCAATCCAGC AGGAGGCTGG GGGAGGAGAG ACTGTGGTGA CTTTTGAGGA CCGGGCCAGG 
CACAGGCAGA GTCAGAGGCG AAGCCGGCAT CGCCGAGTCA GGACTGAGGG CAAGGACTCT 
GCCTCTGCAT CCCGAAGCAG GTCTGCCAGC CAGGAGCGGA GTCTGGATGA AGGGGTGTCC 
GTTGAGGGGG AGAAGGAACA TGAGCCTCAC AGCAGCCACA GGAGCAAAGA GCCGACCATC 
CACGAAGAGG AGAGAACCCA GGACTTAAGG AGGACCAACA GTCTGATGGT CCCCAGGGGC 
TCTGGACTGG TAGGGGCTCT TGATGAAGCA GAAACACCTC TAGTCCAGCC CCAACCTGAG 
CTGGAAGTGG GGAAGGATGC AGCTCTGACA GAGCAGGAGG CTGAAGGCAG CAGTGAACAA 
GCCCTTCTTG GGGACGTACA GCTGGATGTG GGCCGGGGCA TTAGCCAGAG TGAACCGGAT 
CTCTCCTGCA TGACAGCCAA CATGGACAAG GCTACCACGG AGAGCACCAG TGTCACGGTC 
GCCATCCCTG ATGTGGACCC CTTGGTGGAC TCGACAGTGG TGAACATTAG CAACAAGACG 
GATGGAGAAG CCAGTCCCTT GAAGGAGGCA GAGACTAAAG AGGAAGAAGA AGAGGTAGAG 
AAGAAGAAGA AGCAGAAGAA GGAGAAGCGA GAGACTGGAA AAGCCATGGT GCCCCATAGC 
TCTATGTTCA TCTTCAGCAC CACTAACCCG ATCCGCAGGG CCTGCCACTA CATTGTGAAC 
CTACGCTACT TTGAAATGTG CATTCTCTTG GTGATTGCGG CCAGCAGCAT TGCCCTGGCA 
GCCGAGGACC CTGTACTAAC TAACTCGGAG CGTAACAAAG TCCTGAGATA TTTCGACTAT 
GTTTTCACAG GAGTGTTTAC CTTTGAGATG GTTATAAAGA TGATAGACCA AGGCTTGATC 
CTGCAGGATG GATCATACTT CAGAGACCTC TGGAACATCC TGGACTTTGT AGTAGTGGTT 
GGTGCATTGG TAGCATTTGC TCTGGCAACC AACAAGGGGA GGGATATCAA GACTATCAAG 
TCTCTGAGGG TACTTCGAGT TCTGCGGCCA CTGAAAACCA TCAAGCGCTT GCCCAAGCTG 
AAGGCTGTGT TTGACTGCGT GGTGACCTCC TTGAAGAATG TCTTCAACAT ACTCATTGTC 
TACAAGCTCT TCATGTTCAT CTTTGCCGTC ATCGCTGTTC AGCTATTCAA AGGAAAGTTC 
TTTTATTGCA CTGACAGTTC CAAGGACACA GAGAAGGAGT GCATAGGCAA CTATGTAGAC 
CACGAGAAAA ACAAGATGGA GGTGAAGGGC CGGGAATGGA AGCGTCATGA ATTCCACTAT 
GACAACATCA TCTGGGCCCT GCTTACCCTC TTCACAGTCT CTACAGGGGA AGGATGGCCT 
CAGGTTCTGC AGCACTCTGT AGATGTGACA GAGGAGGACA GAGGCCCAAG TCGCAGCAAC 
CGCATGGAGA TGTCCATCTT TTATGTGGTC TACTTTGTGG TCTTCCCTTT CTTCTTTGTC 
AATATCTTCG TGGCTCTCAT TATCATCACC TTCCAGGAGC AAGGGGACAA GATGATGGAG 
GAGTGTAGCC TGGAGAAGAA TGAGCGGGCA TGCATCGACT TCGCCATTAG TGCCAAACCT 
CTCACCCGCT ACATGCCACA GAACAGGCAC ACCTTCCAGT ACCGTGTGTG GCACTTTGTA 
GTCTCCCCAT CCTTTGAGTA TACCATCATG GCCATGATTG CCTTGAACAC TGTCGTGCTG 
ATGATGAAGT ACTACTCTGC TCCTTGCACC TATGAGCTCG CCTTAAAGTA CCTGAACATC 
GCCTTCACTA TGGTGTTTTC CCTGGAGTGT GTCCTAAAGG TCATTGCTTT CGGCTTCCTG 
AACTATTTCC GAGATACCTG GAACATCTTT GACTTCATCA CGGTGATCGG CAGTATCACA 
GAAATTATCC TGACAGATAG CAAGCTGGTG AACACCAGTG GCTTCAATAT GAGCTTTCTG 
AAGCTCTTCC GAGCTGCACG GCTCATAAAG CTTCTGAGAC AGGGCTACAC CATCCGCATC 
TTACTGTGGA CCTTCGTGCA GTCCTTTAAG GCTCTTCCCT ACGTCTGCCT TCTCATTGCC 
ATGCTTTTCT TCATCTACGC CATCATTGGG ATGCAGGTGT TTGGAAACAT AAAGTTAGAT 
GAGGAGAGTC ACATCAACCG GCACAACAAC TTCCGGAGTT TCTTTGGATC CCTCATGCTG 
CTTTTCAGGA GTGCCACGGG GGAGGCTTGG CAGGAGATTA TGCTGTCCTG TCTCGGTGAG 
AAGGGTTGTG AGCCTGACAC CACTGCACCC TCAGGGCAGA ATGAGAGTGA GCGCTGTGGC 
ACTGACCTGG CCTACGTTTA TTTTGTCTCC TTCATCTTCT TCTGCTCCTT CCTGATGCTC 
AACCTGTTCG TGGCTGTCAT CATGGACAAC TTTGAGTACC TGACTCGAGA TTCCTCCATC 
CTGGGGCCTC ACCACTTAGA TGAGTTTGTC CGTGTCTGGG CAGAATATGA CAGAGCAGCA 
TGTGGCCGCA TCCATTACAC TGAGATGTAT GAAATGCTGA CTCTCATGTC ACCACCGCTA 
GGCCTCGGCA AGAGATGTCC CTCCAAAGTG GCCTATAAGA GGCTGGTCCT GATGAACATG 
CCAGTGGCTG AGGACATGAC AGTCCACTTC ACCTCCACAC TTATGGCTCT GATCCGGACA 
GCTCTGGACA TTAAAATCGC CAAAGGTGGT GCAGACAGAC AGCAGCTAGA CTCGGAGCTG 
CAAAAAGAGA CCCTGGCCAT TTGGCCTCAC CTGTCCCAAA AGATGTTGGA TCTGCTTGTG 
CCCATGCCCA AAGCCTCTGA CCTGACTGTG GGCAAGATCT ATGCAGCAAT GATGATCATG 
GACTACTATA AACAGAGTAA GGTAAAGAAA CAGAGACAGC AGCTGGAAGA ACAGAAAAAT 
GCACCCATGT TCCAGCGCAT GGAGCCCTCA TCACTGCCTC AGGAGATCAT TGCTAATGCC 
AAAGCCCTGC CTTATCTCCA GCAGGACCCT GTTTCTGGCC TGAGTGGTCG GAGTGGATAC 
CCTTCAATGA GTCCACTCTC CCCTCAAGAA ATATTCCAGT TGGCTTGTAT GGACCCAGCG 
GATGATGGAC AATTCCAGGA ACAACAATCT CTGGTGAATG CACAAGAGCC AGAGGTTAGT 
GAATTAAAAA GTGTGCAGTC CTCTAACCAT GGCATCTACC TTCCTCCGGA CACCCAGGAG 
CATGCGGGAT CTGGGAGGGC ATCCTCTATG CCACGTCTGA CTATGGATCC CCAGGTGGTA 
ACAGACCCTA GCTCTATGCG ACGTTCATTT TCTACAATTC GGGATAAGCG TTCAAATTCC 
TCCTGGTTGG AGGAGTTCTC CATGGAACGC AGCAGTGAAA ACACTTACAA GTCGCGGCGC 
AGAAGTTACC ATTCCTCCCT GAGGCTGTCA GCACACCGCC TGAATTCTGA CTCTGGTCAC 
AAGTCTGACA CTCACCGATC AGGAGGCAGG GAGAGAGGCC GATCCAAAGA ACGAAAGCAC 
CTCCTCTCTC CAGATGTCTC CCGCTGCAAT TCTGAAGAGC GAGGGACCCA GGCCGACTGG 
GAGTCCCCAG AACGTCGCCA GTCCAGGTCA CCCAGTGAAG GAAGGTCACA GACCCCCAAT 
AGACAGGGTA CCGGTTCCCT AAGTGAGAGC TCCATCCCCT CTATTTCCGA CACCAGCACC 
CCAAGACGAA GCCGTCGCCA GCTCCCACCT GTGCCACCAA AGCCTCGTCC CCTCCTCTCC 
TACAGCTCCC TGATGAGACA CACTGGCGGC ATCTCTCCAC CTCCTGATGG AAGTGAGGGT 
GGATCCCCAC TGGCCTCTCA AGCTCTGGAG AGCAACAGCG CTTGCCTGAC CGAGTCTTCC 
AACTCCTTGC ACCCCCAGCA GGGCCAGCAT CCTTCCCCAC AGCACTACAT CTCCGAGCCC 
TATCTGGCCC TCCATGAAGA CTCCCACGCC TCAGACTGTG GCGAGGAGGA GACACTCACC 
TTTGAGGCAG CCGTAGCCAC TAGCTTGGGC CGGTCCAATA CCATTGGCTC TGCGCCACCC 
CTACGGCACA GCTGGCAGAT GCCTAATGGG CACTATCGGC GGCGGAGGCG TGGGGGGCCT 
GGGCCTGGCA TGATGTGTGG AGCTGTCAGT GACCTCCTGA GTGACACGGA AGAAGACGAT 
AAGTGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017168117.1
CDS	594..7982
Translation	MVPEMRGLSPGRRLPEPTLCPATGGSPEPGAAGRRRHVLALPDEEAWRPQQCALLEANASDEVGMLPASPRSAVAGFDNFFPVQEGEGPGWEGSTTLDAGSSPFLPVSPEVMKRRRGGLIEQRDIIKAHEAHKMQSTPQARRKEWEMARFGEAVVVGRPGSGDGDSDQSRNRQGTPVPASGPAAAYKQSKAQRARTMALYNPIPVRQNCFTVNRSLFIFGEDNIVRKYAKKLIDWPPFEYMILATIIANCIVLALEQHLPEDDKTPMSRRLEKTEPYFIGIFCFEAGIKIVALGFIFHKGSYLRNGWNVMDFIVVLSGILATAGTHFNTHVDLRTLRAVRVLRPLKLVSGIPSLQIVLKSIMKAMVPLLQIGLLLFFAILMFAIIGLEFYSGKLHRACFMNNSGILEGFDPPHPCGVQGCPAGYECKDWIGPNDGITQFDNILFAVLTVFQCITMEGWTTVLYNTNDALGATWNWLYFIPLIIIGSFFVLNLVLGVLSGEFAKERERVENRRAFMKLRRQQQIERELNGYRAWIDKAEEVMLAEENKNSGTSALEVLRRATIKRSRTEAMTRDSSDEHCVDISSVGTPLARASIKSTKVDGASYFRHKERLLRISIRHMVKSQVFYWIVLSVVALNTACVAIVHHNQPQWLTHLLYYAEFLFLGLFLLEMSLKMYGMGPRLYFHSSFNCFDFGVTVGSIFEVVWAIFRPGTSFGISVLRALRLLRIFKITKYWASLRNLVVSLMSSMKSIISLLFLLFLFIVVFALLGMQLFGGRFNFNDGTPSANFDTFPAAIMTVFQILTGEDWNEVMYNGIRSQGGVSSGMWSAIYFIVLTLFGNYTLLNVFLAIAVDNLANAQELTKDEQEEEEAFNQKHALQKAKEVSPMSAPNMPSIERDRRRRHHMSMWEPRSSHLRERRRRHHMSVWEQRTSQLRRHMQMSSQEALNKEEAPPMNPLNPLNPLSPLNPLNAHPSLYRRPRPIEGLALGLGLEKCEEERISRGGSLKGDIGGLTSALDNQRSPLSLGKREPPWLPRSCHGNCDPIQQEAGGGETVVTFEDRARHRQSQRRSRHRRVRTEGKDSASASRSRSASQERSLDEGVSVEGEKEHEPHSSHRSKEPTIHEEERTQDLRRTNSLMVPRGSGLVGALDEAETPLVQPQPELEVGKDAALTEQEAEGSSEQALLGDVQLDVGRGISQSEPDLSCMTANMDKATTESTSVTVAIPDVDPLVDSTVVNISNKTDGEASPLKEAETKEEEEEVEKKKKQKKEKRETGKAMVPHSSMFIFSTTNPIRRACHYIVNLRYFEMCILLVIAASSIALAAEDPVLTNSERNKVLRYFDYVFTGVFTFEMVIKMIDQGLILQDGSYFRDLWNILDFVVVVGALVAFALATNKGRDIKTIKSLRVLRVLRPLKTIKRLPKLKAVFDCVVTSLKNVFNILIVYKLFMFIFAVIAVQLFKGKFFYCTDSSKDTEKECIGNYVDHEKNKMEVKGREWKRHEFHYDNIIWALLTLFTVSTGEGWPQVLQHSVDVTEEDRGPSRSNRMEMSIFYVVYFVVFPFFFVNIFVALIIITFQEQGDKMMEECSLEKNERACIDFAISAKPLTRYMPQNRHTFQYRVWHFVVSPSFEYTIMAMIALNTVVLMMKYYSAPCTYELALKYLNIAFTMVFSLECVLKVIAFGFLNYFRDTWNIFDFITVIGSITEIILTDSKLVNTSGFNMSFLKLFRAARLIKLLRQGYTIRILLWTFVQSFKALPYVCLLIAMLFFIYAIIGMQVFGNIKLDEESHINRHNNFRSFFGSLMLLFRSATGEAWQEIMLSCLGEKGCEPDTTAPSGQNESERCGTDLAYVYFVSFIFFCSFLMLNLFVAVIMDNFEYLTRDSSILGPHHLDEFVRVWAEYDRAACGRIHYTEMYEMLTLMSPPLGLGKRCPSKVAYKRLVLMNMPVAEDMTVHFTSTLMALIRTALDIKIAKGGADRQQLDSELQKETLAIWPHLSQKMLDLLVPMPKASDLTVGKIYAAMMIMDYYKQSKVKKQRQQLEEQKNAPMFQRMEPSSLPQEIIANAKALPYLQQDPVSGLSGRSGYPSMSPLSPQEIFQLACMDPADDGQFQEQQSLVNAQEPEVSELKSVQSSNHGIYLPPDTQEHAGSGRASSMPRLTMDPQVVTDPSSMRRSFSTIRDKRSNSSWLEEFSMERSSENTYKSRRRSYHSSLRLSAHRLNSDSGHKSDTHRSGGRERGRSKERKHLLSPDVSRCNSEERGTQADWESPERRQSRSPSEGRSQTPNRQGTGSLSESSIPSISDTSTPRRSRRQLPPVPPKPRPLLSYSSLMRHTGGISPPPDGSEGGSPLASQALESNSACLTESSNSLHPQQGQHPSPQHYISEPYLALHEDSHASDCGEEETLTFEAAVATSLGRSNTIGSAPPLRHSWQMPNGHYRRRRRGGPGPGMMCGAVSDLLSDTEEDDKC

Target ORF information:

RefSeq Version	XM_017312628.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus calcium channel, voltage-dependent, R type, alpha 1E subunit (Cacna1e), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017312628.1

ORF Insert Sequence:

ATGGTGCCCG AGATGCGTGG ACTGAGCCCA GGCCGAAGGC TGCCCGAGCC CACCCTGTGC 
CCGGCTACAG GAGGGAGCCC TGAGCCCGGG GCTGCTGGCA GGCGCCGCCA CGTCCTGGCT 
CTGCCTGATG AGGAGGCATG GCGGCCGCAG CAGTGCGCCC TGCTCGAGGC CAATGCCTCC 
GACGAGGTGG GCATGTTGCC TGCTTCCCCG CGCTCCGCGG TGGCCGGCTT CGACAACTTC 
TTCCCGGTGC AGGAAGGCGA GGGCCCGGGC TGGGAGGGCA GCACCACGCT GGATGCAGGC 
TCCAGCCCCT TCCTTCCTGT GAGCCCAGAG GTCATGAAGC GGAGGCGAGG GGGGCTCATC 
GAGCAGCGGG ACATCATCAA GGCCCACGAG GCACACAAGA TGCAGAGCAC CCCGCAGGCC 
CGGCGCAAGG AGTGGGAGAT GGCTCGCTTC GGGGAGGCGG TGGTCGTTGG CAGGCCAGGC 
TCAGGCGATG GAGACTCGGA CCAGAGCAGG AACCGACAAG GAACCCCCGT CCCGGCCTCG 
GGGCCGGCGG CCGCCTACAA GCAGTCAAAA GCGCAGAGGG CGCGGACTAT GGCTTTGTAC 
AACCCCATAC CAGTCCGGCA GAACTGTTTC ACGGTCAACA GATCCCTGTT CATCTTCGGA 
GAAGATAACA TTGTCAGGAA ATACGCCAAG AAGCTCATCG ATTGGCCGCC ATTTGAGTAC 
ATGATCCTGG CCACCATCAT TGCCAACTGC ATCGTCTTGG CCCTGGAGCA GCATCTTCCT 
GAGGATGACA AGACCCCAAT GTCTCGAAGA CTGGAGAAGA CAGAACCATA TTTCATTGGG 
ATCTTCTGCT TTGAAGCTGG GATCAAAATT GTGGCTCTAG GGTTCATCTT CCATAAGGGT 
TCATACCTCC GTAATGGCTG GAATGTCATG GACTTCATCG TGGTCCTTAG TGGCATCCTG 
GCCACTGCGG GAACCCACTT CAACACCCAC GTGGACCTGC GGACCCTCCG GGCTGTGCGT 
GTCCTGAGGC CTTTAAAGCT GGTATCAGGA ATACCTAGCC TGCAGATCGT GCTGAAGTCT 
ATCATGAAGG CCATGGTGCC TCTTCTGCAG ATTGGCCTTC TGCTCTTCTT TGCCATCCTC 
ATGTTTGCTA TCATTGGCTT GGAGTTCTAC AGTGGCAAGT TGCATCGAGC ATGCTTCATG 
AACAATTCAG GTATTCTAGA GGGGTTTGAT CCTCCTCACC CGTGTGGTGT GCAAGGCTGC 
CCGGCTGGTT ATGAATGTAA GGACTGGATC GGCCCCAATG ACGGGATCAC CCAGTTTGAC 
AACATCCTTT TTGCCGTGCT GACTGTCTTC CAATGCATCA CTATGGAAGG GTGGACCACT 
GTGCTGTACA ATACCAATGA TGCCTTAGGA GCCACCTGGA ACTGGCTGTA CTTCATCCCT 
CTCATCATCA TCGGATCCTT CTTTGTTCTC AACCTCGTCC TGGGAGTGCT TTCCGGGGAA 
TTTGCCAAAG AAAGAGAGAG AGTGGAGAAC CGAAGAGCGT TCATGAAGCT CCGGCGCCAG 
CAACAGATTG AGCGAGAATT GAACGGCTAC CGTGCCTGGA TAGACAAAGC AGAGGAAGTC 
ATGCTTGCTG AAGAAAATAA AAACTCGGGA ACATCAGCCT TGGAAGTGCT TCGAAGGGCA 
ACCATCAAAA GGAGCCGGAC GGAGGCCATG ACCCGAGACT CCAGCGATGA GCACTGCGTT 
GATATCTCTT CAGTGGGCAC ACCTCTTGCC AGAGCCAGTA TCAAGAGCAC AAAAGTAGAT 
GGGGCCTCCT ATTTCCGGCA CAAGGAACGG CTTCTGCGCA TCTCTATCCG CCACATGGTC 
AAGTCCCAAG TCTTTTACTG GATCGTCCTG AGTGTTGTGG CTCTTAATAC TGCCTGTGTG 
GCCATTGTTC ATCACAACCA GCCACAGTGG CTCACTCACC TCCTCTACTA TGCAGAGTTT 
TTGTTTCTGG GACTCTTCCT GTTGGAGATG TCCCTGAAGA TGTATGGCAT GGGGCCACGC 
CTCTATTTTC ACTCATCCTT CAACTGCTTT GATTTTGGGG TCACAGTGGG CAGCATCTTT 
GAGGTTGTCT GGGCAATCTT CAGACCTGGC ACATCTTTTG GAATCAGTGT CTTACGAGCT 
CTCCGGCTCC TAAGGATATT TAAAATAACC AAGTATTGGG CATCCCTCCG GAATCTGGTT 
GTCTCCTTGA TGAGCTCCAT GAAGTCTATC ATCAGCTTAC TCTTCCTTCT CTTCCTCTTC 
ATCGTTGTCT TTGCTCTCCT GGGAATGCAG CTCTTTGGAG GCAGGTTTAA CTTTAATGAT 
GGGACTCCTT CAGCTAATTT TGACACCTTC CCTGCAGCTA TCATGACAGT GTTCCAGATC 
CTGACAGGGG AAGACTGGAA TGAGGTGATG TACAATGGCA TTCGCTCCCA GGGTGGGGTC 
AGCTCAGGCA TGTGGTCTGC CATCTACTTC ATTGTGCTCA CCTTGTTTGG AAACTACACG 
CTGCTAAATG TATTCTTGGC CATCGCTGTG GACAATCTGG CCAATGCCCA GGAACTGACC 
AAGGATGAGC AGGAGGAAGA AGAAGCCTTC AACCAGAAAC ATGCACTGCA GAAGGCCAAG 
GAGGTCAGCC CGATGTCTGC TCCCAACATG CCTTCCATTG AAAGAGACAG AAGGAGAAGA 
CACCACATGT CGATGTGGGA GCCACGCAGC AGCCACCTGA GGGAGCGGAG GCGCCGGCAC 
CACATGTCTG TGTGGGAACA ACGCACCAGC CAGCTGAGGA GGCACATGCA AATGTCTAGC 
CAAGAAGCCC TCAACAAAGA GGAGGCCCCG CCAATGAACC CCCTCAATCC ACTCAACCCA 
CTGAGTCCTC TCAACCCCCT CAATGCTCAC CCCAGCCTTT ATCGGAGGCC CAGGCCCATT 
GAGGGCCTAG CCCTAGGTCT GGGTCTTGAA AAGTGTGAAG AAGAACGCAT CAGTCGTGGG 
GGCTCCCTCA AGGGGGACAT AGGGGGCCTG ACTAGCGCCC TGGACAACCA GAGGAGCCCC 
TTGTCCTTGG GCAAACGGGA GCCACCGTGG CTGCCTAGAT CCTGTCATGG AAACTGTGAC 
CCAATCCAGC AGGAGGCTGG GGGAGGAGAG ACTGTGGTGA CTTTTGAGGA CCGGGCCAGG 
CACAGGCAGA GTCAGAGGCG AAGCCGGCAT CGCCGAGTCA GGACTGAGGG CAAGGACTCT 
GCCTCTGCAT CCCGAAGCAG GTCTGCCAGC CAGGAGCGGA GTCTGGATGA AGGGGTGTCC 
GTTGAGGGGG AGAAGGAACA TGAGCCTCAC AGCAGCCACA GGAGCAAAGA GCCGACCATC 
CACGAAGAGG AGAGAACCCA GGACTTAAGG AGGACCAACA GTCTGATGGT CCCCAGGGGC 
TCTGGACTGG TAGGGGCTCT TGATGAAGCA GAAACACCTC TAGTCCAGCC CCAACCTGAG 
CTGGAAGTGG GGAAGGATGC AGCTCTGACA GAGCAGGAGG CTGAAGGCAG CAGTGAACAA 
GCCCTTCTTG GGGACGTACA GCTGGATGTG GGCCGGGGCA TTAGCCAGAG TGAACCGGAT 
CTCTCCTGCA TGACAGCCAA CATGGACAAG GCTACCACGG AGAGCACCAG TGTCACGGTC 
GCCATCCCTG ATGTGGACCC CTTGGTGGAC TCGACAGTGG TGAACATTAG CAACAAGACG 
GATGGAGAAG CCAGTCCCTT GAAGGAGGCA GAGACTAAAG AGGAAGAAGA AGAGGTAGAG 
AAGAAGAAGA AGCAGAAGAA GGAGAAGCGA GAGACTGGAA AAGCCATGGT GCCCCATAGC 
TCTATGTTCA TCTTCAGCAC CACTAACCCG ATCCGCAGGG CCTGCCACTA CATTGTGAAC 
CTACGCTACT TTGAAATGTG CATTCTCTTG GTGATTGCGG CCAGCAGCAT TGCCCTGGCA 
GCCGAGGACC CTGTACTAAC TAACTCGGAG CGTAACAAAG TCCTGAGATA TTTCGACTAT 
GTTTTCACAG GAGTGTTTAC CTTTGAGATG GTTATAAAGA TGATAGACCA AGGCTTGATC 
CTGCAGGATG GATCATACTT CAGAGACCTC TGGAACATCC TGGACTTTGT AGTAGTGGTT 
GGTGCATTGG TAGCATTTGC TCTGGCAACC AACAAGGGGA GGGATATCAA GACTATCAAG 
TCTCTGAGGG TACTTCGAGT TCTGCGGCCA CTGAAAACCA TCAAGCGCTT GCCCAAGCTG 
AAGGCTGTGT TTGACTGCGT GGTGACCTCC TTGAAGAATG TCTTCAACAT ACTCATTGTC 
TACAAGCTCT TCATGTTCAT CTTTGCCGTC ATCGCTGTTC AGCTATTCAA AGGAAAGTTC 
TTTTATTGCA CTGACAGTTC CAAGGACACA GAGAAGGAGT GCATAGGCAA CTATGTAGAC 
CACGAGAAAA ACAAGATGGA GGTGAAGGGC CGGGAATGGA AGCGTCATGA ATTCCACTAT 
GACAACATCA TCTGGGCCCT GCTTACCCTC TTCACAGTCT CTACAGGGGA AGGATGGCCT 
CAGGTTCTGC AGCACTCTGT AGATGTGACA GAGGAGGACA GAGGCCCAAG TCGCAGCAAC 
CGCATGGAGA TGTCCATCTT TTATGTGGTC TACTTTGTGG TCTTCCCTTT CTTCTTTGTC 
AATATCTTCG TGGCTCTCAT TATCATCACC TTCCAGGAGC AAGGGGACAA GATGATGGAG 
GAGTGTAGCC TGGAGAAGAA TGAGCGGGCA TGCATCGACT TCGCCATTAG TGCCAAACCT 
CTCACCCGCT ACATGCCACA GAACAGGCAC ACCTTCCAGT ACCGTGTGTG GCACTTTGTA 
GTCTCCCCAT CCTTTGAGTA TACCATCATG GCCATGATTG CCTTGAACAC TGTCGTGCTG 
ATGATGAAGT ACTACTCTGC TCCTTGCACC TATGAGCTCG CCTTAAAGTA CCTGAACATC 
GCCTTCACTA TGGTGTTTTC CCTGGAGTGT GTCCTAAAGG TCATTGCTTT CGGCTTCCTG 
AACTATTTCC GAGATACCTG GAACATCTTT GACTTCATCA CGGTGATCGG CAGTATCACA 
GAAATTATCC TGACAGATAG CAAGCTGGTG AACACCAGTG GCTTCAATAT GAGCTTTCTG 
AAGCTCTTCC GAGCTGCACG GCTCATAAAG CTTCTGAGAC AGGGCTACAC CATCCGCATC 
TTACTGTGGA CCTTCGTGCA GTCCTTTAAG GCTCTTCCCT ACGTCTGCCT TCTCATTGCC 
ATGCTTTTCT TCATCTACGC CATCATTGGG ATGCAGGTGT TTGGAAACAT AAAGTTAGAT 
GAGGAGAGTC ACATCAACCG GCACAACAAC TTCCGGAGTT TCTTTGGATC CCTCATGCTG 
CTTTTCAGGA GTGCCACGGG GGAGGCTTGG CAGGAGATTA TGCTGTCCTG TCTCGGTGAG 
AAGGGTTGTG AGCCTGACAC CACTGCACCC TCAGGGCAGA ATGAGAGTGA GCGCTGTGGC 
ACTGACCTGG CCTACGTTTA TTTTGTCTCC TTCATCTTCT TCTGCTCCTT CCTGATGCTC 
AACCTGTTCG TGGCTGTCAT CATGGACAAC TTTGAGTACC TGACTCGAGA TTCCTCCATC 
CTGGGGCCTC ACCACTTAGA TGAGTTTGTC CGTGTCTGGG CAGAATATGA CAGAGCAGCA 
TGTGGCCGCA TCCATTACAC TGAGATGTAT GAAATGCTGA CTCTCATGTC ACCACCGCTA 
GGCCTCGGCA AGAGATGTCC CTCCAAAGTG GCCTATAAGA GGCTGGTCCT GATGAACATG 
CCAGTGGCTG AGGACATGAC AGTCCACTTC ACCTCCACAC TTATGGCTCT GATCCGGACA 
GCTCTGGACA TTAAAATCGC CAAAGGTGGT GCAGACAGAC AGCAGCTAGA CTCGGAGCTG 
CAAAAAGAGA CCCTGGCCAT TTGGCCTCAC CTGTCCCAAA AGATGTTGGA TCTGCTTGTG 
CCCATGCCCA AAGCCTCTGA CCTGACTGTG GGCAAGATCT ATGCAGCAAT GATGATCATG 
GACTACTATA AACAGAGTAA GGTAAAGAAA CAGAGACAGC AGCTGGAAGA ACAGAAAAAT 
GCACCCATGT TCCAGCGCAT GGAGCCCTCA TCACTGCCTC AGGAGATCAT TGCTAATGCC 
AAAGCCCTGC CTTATCTCCA GCAGGACCCT GTTTCTGGCC TGAGTGGTCG GAGTGGATAC 
CCTTCAATGA GTCCACTCTC CCCTCAAGAA ATATTCCAGT TGGCTTGTAT GGACCCAGCG 
GATGATGGAC AATTCCAGGA ACAACAATCT CTGGTGAATG CACAAGAGCC AGAGGTTAGT 
GAATTAAAAA GTGTGCAGTC CTCTAACCAT GGCATCTACC TTCCTCCGGA CACCCAGGAG 
CATGCGGGAT CTGGGAGGGC ATCCTCTATG CCACGTCTGA CTATGGATCC CCAGGTGGTA 
ACAGACCCTA GCTCTATGCG ACGTTCATTT TCTACAATTC GGGATAAGCG TTCAAATTCC 
TCCTGGTTGG AGGAGTTCTC CATGGAACGC AGCAGTGAAA ACACTTACAA GTCGCGGCGC 
AGAAGTTACC ATTCCTCCCT GAGGCTGTCA GCACACCGCC TGAATTCTGA CTCTGGTCAC 
AAGTCTGACA CTCACCGATC AGGAGGCAGG GAGAGAGGCC GATCCAAAGA ACGAAAGCAC 
CTCCTCTCTC CAGATGTCTC CCGCTGCAAT TCTGAAGAGC GAGGGACCCA GGCCGACTGG 
GAGTCCCCAG AACGTCGCCA GTCCAGGTCA CCCAGTGAAG GAAGGTCACA GACCCCCAAT 
AGACAGGGTA CCGGTTCCCT AAGTGAGAGC TCCATCCCCT CTATTTCCGA CACCAGCACC 
CCAAGACGAA GCCGTCGCCA GCTCCCACCT GTGCCACCAA AGCCTCGTCC CCTCCTCTCC 
TACAGCTCCC TGATGAGACA CACTGGCGGC ATCTCTCCAC CTCCTGATGG AAGTGAGGGT 
GGATCCCCAC TGGCCTCTCA AGCTCTGGAG AGCAACAGCG CTTGCCTGAC CGAGTCTTCC 
AACTCCTTGC ACCCCCAGCA GGGCCAGCAT CCTTCCCCAC AGCACTACAT CTCCGAGCCC 
TATCTGGCCC TCCATGAAGA CTCCCACGCC TCAGACTGTG GCGAGGAGGA GACACTCACC 
TTTGAGGCAG CCGTAGCCAC TAGCTTGGGC CGGTCCAATA CCATTGGCTC TGCGCCACCC 
CTACGGCACA GCTGGCAGAT GCCTAATGGG CACTATCGGC GGCGGAGGCG TGGGGGGCCT 
GGGCCTGGCA TGATGTGTGG AGCTGTCAGT GACCTCCTGA GTGACACGGA AGAAGACGAT 
AAGTGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cacna1e ( XM_017312642.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017168131.1 Mus musculus calcium channel, voltage-dependent, R type, alpha 1E subunit (Cacna1e), transcript variant X8, mRNA.

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CloneID	OMu67263
Clone ID Related Accession (Same CDS sequence)	XM_017312642.1
Accession Version	XM_017312642.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7389bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	voltage-dependent R-type calcium channel subunit alpha-1E isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGCCCG AGATGCGTGG ACTGAGCCCA GGCCGAAGGC TGCCCGAGCC CACCCTGTGC 
CCGGCTACAG GAGGGAGCCC TGAGCCCGGG GCTGCTGGCA GGCGCCGCCA CGTCCTGGCT 
CTGCCTGATG AGGAGGCATG GCGGCCGCAG CAGTGCGCCC TGCTCGAGGC CAATGCCTCC 
GACGAGGTGG GCATGTTGCC TGCTTCCCCG CGCTCCGCGG TGGCCGGCTT CGACAACTTC 
TTCCCGGTGC AGGAAGGCGA GGGCCCGGGC TGGGAGGGCA GCACCACGCT GGATGCAGGC 
TCCAGCCCCT TCCTTCCTGT GAGCCCAGAG GTCATGAAGC GGAGGCGAGG GGGGCTCATC 
GAGCAGCGGG ACATCATCAA GGCCCACGAG GCACACAAGA TGCAGAGCAC CCCGCAGGCC 
CGGCGCAAGG AGTGGGAGAT GGCTCGCTTC GGGGAGGCGG TGGTCGTTGG CAGGCCAGGC 
TCAGGCGATG GAGACTCGGA CCAGAGCAGG AACCGACAAG GAACCCCCGT CCCGGCCTCG 
GGGCCGGCGG CCGCCTACAA GCAGTCAAAA GCGCAGAGGG CGCGGACTAT GGCTTTGTAC 
AACCCCATAC CAGTCCGGCA GAACTGTTTC ACGGTCAACA GATCCCTGTT CATCTTCGGA 
GAAGATAACA TTGTCAGGAA ATACGCCAAG AAGCTCATCG ATTGGCCGCC ATTTGAGTAC 
ATGATCCTGG CCACCATCAT TGCCAACTGC ATCGTCTTGG CCCTGGAGCA GCATCTTCCT 
GAGGATGACA AGACCCCAAT GTCTCGAAGA CTGGAGAAGA CAGAACCATA TTTCATTGGG 
ATCTTCTGCT TTGAAGCTGG GATCAAAATT GTGGCTCTAG GGTTCATCTT CCATAAGGGT 
TCATACCTCC GTAATGGCTG GAATGTCATG GACTTCATCG TGGTCCTTAG TGGCATCCTG 
GCCACTGCGG GAACCCACTT CAACACCCAC GTGGACCTGC GGACCCTCCG GGCTGTGCGT 
GTCCTGAGGC CTTTAAAGCT GGTATCAGGA ATACCTAGCC TGCAGATCGT GCTGAAGTCT 
ATCATGAAGG CCATGGTGCC TCTTCTGCAG ATTGGCCTTC TGCTCTTCTT TGCCATCCTC 
ATGTTTGCTA TCATTGGCTT GGAGTTCTAC AGTGGCAAGT TGCATCGAGC ATGCTTCATG 
AACAATTCAG GTATTCTAGA GGGGTTTGAT CCTCCTCACC CGTGTGGTGT GCAAGGCTGC 
CCGGCTGGTT ATGAATGTAA GGACTGGATC GGCCCCAATG ACGGGATCAC CCAGTTTGAC 
AACATCCTTT TTGCCGTGCT GACTGTCTTC CAATGCATCA CTATGGAAGG GTGGACCACT 
GTGCTGTACA ATACCAATGA TGCCTTAGGA GCCACCTGGA ACTGGCTGTA CTTCATCCCT 
CTCATCATCA TCGGATCCTT CTTTGTTCTC AACCTCGTCC TGGGAGTGCT TTCCGGGGAA 
TTTGCCAAAG AAAGAGAGAG AGTGGAGAAC CGAAGAGCGT TCATGAAGCT CCGGCGCCAG 
CAACAGATTG AGCGAGAATT GAACGGCTAC CGTGCCTGGA TAGACAAAGC AGAGGAAGTC 
ATGCTTGCTG AAGAAAATAA AAACTCGGGA ACATCAGCCT TGGAAGTGCT TCGAAGGGCA 
ACCATCAAAA GGAGCCGGAC GGAGGCCATG ACCCGAGACT CCAGCGATGA GCACTGCGTT 
GATATCTCTT CAGTGGGCAC ACCTCTTGCC AGAGCCAGTA TCAAGAGCAC AAAAGTAGAT 
GGGGCCTCCT ATTTCCGGCA CAAGGAACGG CTTCTGCGCA TCTCTATCCG CCACATGGTC 
AAGTCCCAAG TCTTTTACTG GATCGTCCTG AGTGTTGTGG CTCTTAATAC TGCCTGTGTG 
GCCATTGTTC ATCACAACCA GCCACAGTGG CTCACTCACC TCCTCTACTA TGCAGAGTTT 
TTGTTTCTGG GACTCTTCCT GTTGGAGATG TCCCTGAAGA TGTATGGCAT GGGGCCACGC 
CTCTATTTTC ACTCATCCTT CAACTGCTTT GATTTTGGGG TCACAGTGGG CAGCATCTTT 
GAGGTTGTCT GGGCAATCTT CAGACCTGGC ACATCTTTTG GAATCAGTGT CTTACGAGCT 
CTCCGGCTCC TAAGGATATT TAAAATAACC AAGTATTGGG CATCCCTCCG GAATCTGGTT 
GTCTCCTTGA TGAGCTCCAT GAAGTCTATC ATCAGCTTAC TCTTCCTTCT CTTCCTCTTC 
ATCGTTGTCT TTGCTCTCCT GGGAATGCAG CTCTTTGGAG GCAGGTTTAA CTTTAATGAT 
GGGACTCCTT CAGCTAATTT TGACACCTTC CCTGCAGCTA TCATGACAGT GTTCCAGATC 
CTGACAGGGG AAGACTGGAA TGAGGTGATG TACAATGGCA TTCGCTCCCA GGGTGGGGTC 
AGCTCAGGCA TGTGGTCTGC CATCTACTTC ATTGTGCTCA CCTTGTTTGG AAACTACACG 
CTGCTAAATG TATTCTTGGC CATCGCTGTG GACAATCTGG CCAATGCCCA GGAACTGACC 
AAGGATGAGC AGGAGGAAGA AGAAGCCTTC AACCAGAAAC ATGCACTGCA GAAGGCCAAG 
GAGGTCAGCC CGATGTCTGC TCCCAACATG CCTTCCATTG AAAGAGACAG AAGGAGAAGA 
CACCACATGT CGATGTGGGA GCCACGCAGC AGCCACCTGA GGGAGCGGAG GCGCCGGCAC 
CACATGTCTG TGTGGGAACA ACGCACCAGC CAGCTGAGGA GGCACATGCA AATGTCTAGC 
CAAGAAGCCC TCAACAAAGA GGAGGCCCCG CCAATGAACC CCCTCAATCC ACTCAACCCA 
CTGAGTCCTC TCAACCCCCT CAATGCTCAC CCCAGCCTTT ATCGGAGGCC CAGGCCCATT 
GAGGGCCTAG CCCTAGGTCT GGGTCTTGAA AAGTGTGAAG AAGAACGCAT CAGTCGTGGG 
GGCTCCCTCA AGGGGGACAT AGGGGGCCTG ACTAGCGCCC TGGACAACCA GAGGAGCCCC 
TTGTCCTTGG GCAAACGGGA GCCACCGTGG CTGCCTAGAT CCTGTCATGG AAACTGTGAC 
CCAATCCAGC AGGAGGCTGG GGGAGGAGAG ACTGTGGTGA CTTTTGAGGA CCGGGCCAGG 
CACAGGCAGA GTCAGAGGCG AAGCCGGCAT CGCCGAGTCA GGACTGAGGG CAAGGACTCT 
GCCTCTGCAT CCCGAAGCAG GTCTGCCAGC CAGGAGCGGA GTCTGGATGA AGGGGTGTCC 
GTTGAGGGGG AGAAGGAACA TGAGCCTCAC AGCAGCCACA GGAGCAAAGA GCCGACCATC 
CACGAAGAGG AGAGAACCCA GGACTTAAGG AGGACCAACA GTCTGATGGT CCCCAGGGGC 
TCTGGACTGG TAGGGGCTCT TGATGAAGCA GAAACACCTC TAGTCCAGCC CCAACCTGAG 
CTGGAAGTGG GGAAGGATGC AGCTCTGACA GAGCAGGAGG CTGAAGGCAG CAGTGAACAA 
GCCCTTCTTG GGGACGTACA GCTGGATGTG GGCCGGGGCA TTAGCCAGAG TGAACCGGAT 
CTCTCCTGCA TGACAGCCAA CATGGACAAG GCTACCACGG AGAGCACCAG TGTCACGGTC 
GCCATCCCTG ATGTGGACCC CTTGGTGGAC TCGACAGTGG TGAACATTAG CAACAAGACG 
GATGGAGAAG CCAGTCCCTT GAAGGAGGCA GAGACTAAAG AGGAAGAAGA AGAGGTAGAG 
AAGAAGAAGA AGCAGAAGAA GGAGAAGCGA GAGACTGGAA AAGCCATGGT GCCCCATAGC 
TCTATGTTCA TCTTCAGCAC CACTAACCCG ATCCGCAGGG CCTGCCACTA CATTGTGAAC 
CTACGCTACT TTGAAATGTG CATTCTCTTG GTGATTGCGG CCAGCAGCAT TGCCCTGGCA 
GCCGAGGACC CTGTACTAAC TAACTCGGAG CGTAACAAAG TCCTGAGATA TTTCGACTAT 
GTTTTCACAG GAGTGTTTAC CTTTGAGATG GTTATAAAGA TGATAGACCA AGGCTTGATC 
CTGCAGGATG GATCATACTT CAGAGACCTC TGGAACATCC TGGACTTTGT AGTAGTGGTT 
GGTGCATTGG TAGCATTTGC TCTGGCAACC AACAAGGGGA GGGATATCAA GACTATCAAG 
TCTCTGAGGG TACTTCGAGT TCTGCGGCCA CTGAAAACCA TCAAGCGCTT GCCCAAGCTG 
AAGGCTGTGT TTGACTGCGT GGTGACCTCC TTGAAGAATG TCTTCAACAT ACTCATTGTC 
TACAAGCTCT TCATGTTCAT CTTTGCCGTC ATCGCTGTTC AGCTATTCAA AGGAAAGTTC 
TTTTATTGCA CTGACAGTTC CAAGGACACA GAGAAGGAGT GCATAGGCAA CTATGTAGAC 
CACGAGAAAA ACAAGATGGA GGTGAAGGGC CGGGAATGGA AGCGTCATGA ATTCCACTAT 
GACAACATCA TCTGGGCCCT GCTTACCCTC TTCACAGTCT CTACAGGGGA AGGATGGCCT 
CAGGTTCTGC AGCACTCTGT AGATGTGACA GAGGAGGACA GAGGCCCAAG TCGCAGCAAC 
CGCATGGAGA TGTCCATCTT TTATGTGGTC TACTTTGTGG TCTTCCCTTT CTTCTTTGTC 
AATATCTTCG TGGCTCTCAT TATCATCACC TTCCAGGAGC AAGGGGACAA GATGATGGAG 
GAGTGTAGCC TGGAGAAGAA TGAGCGGGCA TGCATCGACT TCGCCATTAG TGCCAAACCT 
CTCACCCGCT ACATGCCACA GAACAGGCAC ACCTTCCAGT ACCGTGTGTG GCACTTTGTA 
GTCTCCCCAT CCTTTGAGTA TACCATCATG GCCATGATTG CCTTGAACAC TGTCGTGCTG 
ATGATGAAGT ACTACTCTGC TCCTTGCACC TATGAGCTCG CCTTAAAGTA CCTGAACATC 
GCCTTCACTA TGGTGTTTTC CCTGGAGTGT GTCCTAAAGG TCATTGCTTT CGGCTTCCTG 
AACTATTTCC GAGATACCTG GAACATCTTT GACTTCATCA CGGTGATCGG CAGTATCACA 
GAAATTATCC TGACAGATAG CAAGCTGGTG AACACCAGTG GCTTCAATAT GAGCTTTCTG 
AAGCTCTTCC GAGCTGCACG GCTCATAAAG CTTCTGAGAC AGGGCTACAC CATCCGCATC 
TTACTGTGGA CCTTCGTGCA GTCCTTTAAG GCTCTTCCCT ACGTCTGCCT TCTCATTGCC 
ATGCTTTTCT TCATCTACGC CATCATTGGG ATGCAGGTGT TTGGAAACAT AAAGTTAGAT 
GAGGAGAGTC ACATCAACCG GCACAACAAC TTCCGGAGTT TCTTTGGATC CCTCATGCTG 
CTTTTCAGGA GTGCCACGGG GGAGGCTTGG CAGGAGATTA TGCTGTCCTG TCTCGGTGAG 
AAGGGTTGTG AGCCTGACAC CACTGCACCC TCAGGGCAGA ATGAGAGTGA GCGCTGTGGC 
ACTGACCTGG CCTACGTTTA TTTTGTCTCC TTCATCTTCT TCTGCTCCTT CCTGATGCTC 
AACCTGTTCG TGGCTGTCAT CATGGACAAC TTTGAGTACC TGACTCGAGA TTCCTCCATC 
CTGGGGCCTC ACCACTTAGA TGAGTTTGTC CGTGTCTGGG CAGAATATGA CAGAGCAGCA 
TGCGGCAGGA TCCCGTATAA GGATATGTAC AAATTAGTGC GGGTGATCTC GCCTCCTCTG 
GGCCTGGGAG AGAACTGCCC CTACAGGGTG GCTTGCAAGA GGCTGGTCCT GATGAACATG 
CCAGTGGCTG AGGACATGAC AGTCCACTTC ACCTCCACAC TTATGGCTCT GATCCGGACA 
GCTCTGGACA TTAAAATCGC CAAAGGTGGT GCAGACAGAC AGCAGCTAGA CTCGGAGCTG 
CAAAAAGAGA CCCTGGCCAT TTGGCCTCAC CTGTCCCAAA AGATGTTGGA TCTGCTTGTG 
CCCATGCCCA AAGCCTCTGA CCTGACTGTG GGCAAGATCT ATGCAGCAAT GATGATCATG 
GACTACTATA AACAGAGTAA GGTAAAGAAA CAGAGACAGC AGCTGGAAGA ACAGAAAAAT 
GCACCCATGT TCCAGCGCAT GGAGCCCTCA TCACTGCCTC AGGAGATCAT TGCTAATGCC 
AAAGCCCTGC CTTATCTCCA GCAGGACCCT GTTTCTGGCC TGAGTGGTCG GAGTGGATAC 
CCTTCAATGA GTCCACTCTC CCCTCAAGAA ATATTCCAGT TGGCTTGTAT GGACCCAGCG 
GATGATGGAC AATTCCAGGA ACAACAATCT CTGGTGAATG CACAAGAGCC AGAGGTTAGT 
GAATTAAAAA GTGTGCAGTC CTCTAACCAT GGCATCTACC TTCCTCCGGA CACCCAGGAG 
CATGCGGGAT CTGGGAGGGC ATCCTCTATG CCACGTCTGA CTATGGATCC CCAGGTGGTA 
ACAGACCCTA GCTCTATGCG ACGTTCATTT TCTACAATTC GGGATAAGCG TTCAAATTCC 
TCCTGGTTGG AGGAGTTCTC CATGGAACGC AGCAGTGAAA ACACTTACAA GTCGCGGCGC 
AGAAGTTACC ATTCCTCCCT GAGGCTGTCA GCACACCGCC TGAATTCTGA CTCTGGTCAC 
AAGTCTGACA CTCACCGATC AGGAGGCAGG GAGAGAGGCC GATCCAAAGA ACGAAAGCAC 
CTCCTCTCTC CAGATGTCTC CCGCTGCAAT TCTGAAGAGC GAGGGACCCA GGCCGACTGG 
GAGTCCCCAG AACGTCGCCA GTCCAGGTCA CCCAGTGAAG GAAGGTCACA GACCCCCAAT 
AGACAGGGTA CCGGTTCCCT AAGTGAGAGC TCCATCCCCT CTATTTCCGA CACCAGCACC 
CCAAGACGAA GCCGTCGCCA GCTCCCACCT GTGCCACCAA AGCCTCGTCC CCTCCTCTCC 
TACAGCTCCC TGATGAGACA CACTGGCGGC ATCTCTCCAC CTCCTGATGG AAGTGAGGGT 
GGATCCCCAC TGGCCTCTCA AGCTCTGGAG AGCAACAGCG CTTGCCTGAC CGAGTCTTCC 
AACTCCTTGC ACCCCCAGCA GGGCCAGCAT CCTTCCCCAC AGCACTACAT CTCCGAGCCC 
TATCTGGCCC TCCATGAAGA CTCCCACGCC TCAGACTGTG GCGAGGAGGA GACACTCACC 
TTTGAGGCAG CCGTAGCCAC TAGCTTGGGC CGGTCCAATA CCATTGGCTC TGCGCCACCC 
CTACGGCACA GCTGGCAGAT GCCTAATGGG CACTATCGGC GGCGGAGGCG TGGGGGGCCT 
GGGCCTGGCA TGATGTGTGG AGCTGTCAGT GACCTCCTGA GTGACACGGA AGAAGACGAT 
AAGTGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017168131.1
CDS	594..7982
Translation	MVPEMRGLSPGRRLPEPTLCPATGGSPEPGAAGRRRHVLALPDEEAWRPQQCALLEANASDEVGMLPASPRSAVAGFDNFFPVQEGEGPGWEGSTTLDAGSSPFLPVSPEVMKRRRGGLIEQRDIIKAHEAHKMQSTPQARRKEWEMARFGEAVVVGRPGSGDGDSDQSRNRQGTPVPASGPAAAYKQSKAQRARTMALYNPIPVRQNCFTVNRSLFIFGEDNIVRKYAKKLIDWPPFEYMILATIIANCIVLALEQHLPEDDKTPMSRRLEKTEPYFIGIFCFEAGIKIVALGFIFHKGSYLRNGWNVMDFIVVLSGILATAGTHFNTHVDLRTLRAVRVLRPLKLVSGIPSLQIVLKSIMKAMVPLLQIGLLLFFAILMFAIIGLEFYSGKLHRACFMNNSGILEGFDPPHPCGVQGCPAGYECKDWIGPNDGITQFDNILFAVLTVFQCITMEGWTTVLYNTNDALGATWNWLYFIPLIIIGSFFVLNLVLGVLSGEFAKERERVENRRAFMKLRRQQQIERELNGYRAWIDKAEEVMLAEENKNSGTSALEVLRRATIKRSRTEAMTRDSSDEHCVDISSVGTPLARASIKSTKVDGASYFRHKERLLRISIRHMVKSQVFYWIVLSVVALNTACVAIVHHNQPQWLTHLLYYAEFLFLGLFLLEMSLKMYGMGPRLYFHSSFNCFDFGVTVGSIFEVVWAIFRPGTSFGISVLRALRLLRIFKITKYWASLRNLVVSLMSSMKSIISLLFLLFLFIVVFALLGMQLFGGRFNFNDGTPSANFDTFPAAIMTVFQILTGEDWNEVMYNGIRSQGGVSSGMWSAIYFIVLTLFGNYTLLNVFLAIAVDNLANAQELTKDEQEEEEAFNQKHALQKAKEVSPMSAPNMPSIERDRRRRHHMSMWEPRSSHLRERRRRHHMSVWEQRTSQLRRHMQMSSQEALNKEEAPPMNPLNPLNPLSPLNPLNAHPSLYRRPRPIEGLALGLGLEKCEEERISRGGSLKGDIGGLTSALDNQRSPLSLGKREPPWLPRSCHGNCDPIQQEAGGGETVVTFEDRARHRQSQRRSRHRRVRTEGKDSASASRSRSASQERSLDEGVSVEGEKEHEPHSSHRSKEPTIHEEERTQDLRRTNSLMVPRGSGLVGALDEAETPLVQPQPELEVGKDAALTEQEAEGSSEQALLGDVQLDVGRGISQSEPDLSCMTANMDKATTESTSVTVAIPDVDPLVDSTVVNISNKTDGEASPLKEAETKEEEEEVEKKKKQKKEKRETGKAMVPHSSMFIFSTTNPIRRACHYIVNLRYFEMCILLVIAASSIALAAEDPVLTNSERNKVLRYFDYVFTGVFTFEMVIKMIDQGLILQDGSYFRDLWNILDFVVVVGALVAFALATNKGRDIKTIKSLRVLRVLRPLKTIKRLPKLKAVFDCVVTSLKNVFNILIVYKLFMFIFAVIAVQLFKGKFFYCTDSSKDTEKECIGNYVDHEKNKMEVKGREWKRHEFHYDNIIWALLTLFTVSTGEGWPQVLQHSVDVTEEDRGPSRSNRMEMSIFYVVYFVVFPFFFVNIFVALIIITFQEQGDKMMEECSLEKNERACIDFAISAKPLTRYMPQNRHTFQYRVWHFVVSPSFEYTIMAMIALNTVVLMMKYYSAPCTYELALKYLNIAFTMVFSLECVLKVIAFGFLNYFRDTWNIFDFITVIGSITEIILTDSKLVNTSGFNMSFLKLFRAARLIKLLRQGYTIRILLWTFVQSFKALPYVCLLIAMLFFIYAIIGMQVFGNIKLDEESHINRHNNFRSFFGSLMLLFRSATGEAWQEIMLSCLGEKGCEPDTTAPSGQNESERCGTDLAYVYFVSFIFFCSFLMLNLFVAVIMDNFEYLTRDSSILGPHHLDEFVRVWAEYDRAACGRIPYKDMYKLVRVISPPLGLGENCPYRVACKRLVLMNMPVAEDMTVHFTSTLMALIRTALDIKIAKGGADRQQLDSELQKETLAIWPHLSQKMLDLLVPMPKASDLTVGKIYAAMMIMDYYKQSKVKKQRQQLEEQKNAPMFQRMEPSSLPQEIIANAKALPYLQQDPVSGLSGRSGYPSMSPLSPQEIFQLACMDPADDGQFQEQQSLVNAQEPEVSELKSVQSSNHGIYLPPDTQEHAGSGRASSMPRLTMDPQVVTDPSSMRRSFSTIRDKRSNSSWLEEFSMERSSENTYKSRRRSYHSSLRLSAHRLNSDSGHKSDTHRSGGRERGRSKERKHLLSPDVSRCNSEERGTQADWESPERRQSRSPSEGRSQTPNRQGTGSLSESSIPSISDTSTPRRSRRQLPPVPPKPRPLLSYSSLMRHTGGISPPPDGSEGGSPLASQALESNSACLTESSNSLHPQQGQHPSPQHYISEPYLALHEDSHASDCGEEETLTFEAAVATSLGRSNTIGSAPPLRHSWQMPNGHYRRRRRGGPGPGMMCGAVSDLLSDTEEDDKC

Target ORF information:

RefSeq Version	XM_017312642.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus calcium channel, voltage-dependent, R type, alpha 1E subunit (Cacna1e), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017312642.1

ORF Insert Sequence:

ATGGTGCCCG AGATGCGTGG ACTGAGCCCA GGCCGAAGGC TGCCCGAGCC CACCCTGTGC 
CCGGCTACAG GAGGGAGCCC TGAGCCCGGG GCTGCTGGCA GGCGCCGCCA CGTCCTGGCT 
CTGCCTGATG AGGAGGCATG GCGGCCGCAG CAGTGCGCCC TGCTCGAGGC CAATGCCTCC 
GACGAGGTGG GCATGTTGCC TGCTTCCCCG CGCTCCGCGG TGGCCGGCTT CGACAACTTC 
TTCCCGGTGC AGGAAGGCGA GGGCCCGGGC TGGGAGGGCA GCACCACGCT GGATGCAGGC 
TCCAGCCCCT TCCTTCCTGT GAGCCCAGAG GTCATGAAGC GGAGGCGAGG GGGGCTCATC 
GAGCAGCGGG ACATCATCAA GGCCCACGAG GCACACAAGA TGCAGAGCAC CCCGCAGGCC 
CGGCGCAAGG AGTGGGAGAT GGCTCGCTTC GGGGAGGCGG TGGTCGTTGG CAGGCCAGGC 
TCAGGCGATG GAGACTCGGA CCAGAGCAGG AACCGACAAG GAACCCCCGT CCCGGCCTCG 
GGGCCGGCGG CCGCCTACAA GCAGTCAAAA GCGCAGAGGG CGCGGACTAT GGCTTTGTAC 
AACCCCATAC CAGTCCGGCA GAACTGTTTC ACGGTCAACA GATCCCTGTT CATCTTCGGA 
GAAGATAACA TTGTCAGGAA ATACGCCAAG AAGCTCATCG ATTGGCCGCC ATTTGAGTAC 
ATGATCCTGG CCACCATCAT TGCCAACTGC ATCGTCTTGG CCCTGGAGCA GCATCTTCCT 
GAGGATGACA AGACCCCAAT GTCTCGAAGA CTGGAGAAGA CAGAACCATA TTTCATTGGG 
ATCTTCTGCT TTGAAGCTGG GATCAAAATT GTGGCTCTAG GGTTCATCTT CCATAAGGGT 
TCATACCTCC GTAATGGCTG GAATGTCATG GACTTCATCG TGGTCCTTAG TGGCATCCTG 
GCCACTGCGG GAACCCACTT CAACACCCAC GTGGACCTGC GGACCCTCCG GGCTGTGCGT 
GTCCTGAGGC CTTTAAAGCT GGTATCAGGA ATACCTAGCC TGCAGATCGT GCTGAAGTCT 
ATCATGAAGG CCATGGTGCC TCTTCTGCAG ATTGGCCTTC TGCTCTTCTT TGCCATCCTC 
ATGTTTGCTA TCATTGGCTT GGAGTTCTAC AGTGGCAAGT TGCATCGAGC ATGCTTCATG 
AACAATTCAG GTATTCTAGA GGGGTTTGAT CCTCCTCACC CGTGTGGTGT GCAAGGCTGC 
CCGGCTGGTT ATGAATGTAA GGACTGGATC GGCCCCAATG ACGGGATCAC CCAGTTTGAC 
AACATCCTTT TTGCCGTGCT GACTGTCTTC CAATGCATCA CTATGGAAGG GTGGACCACT 
GTGCTGTACA ATACCAATGA TGCCTTAGGA GCCACCTGGA ACTGGCTGTA CTTCATCCCT 
CTCATCATCA TCGGATCCTT CTTTGTTCTC AACCTCGTCC TGGGAGTGCT TTCCGGGGAA 
TTTGCCAAAG AAAGAGAGAG AGTGGAGAAC CGAAGAGCGT TCATGAAGCT CCGGCGCCAG 
CAACAGATTG AGCGAGAATT GAACGGCTAC CGTGCCTGGA TAGACAAAGC AGAGGAAGTC 
ATGCTTGCTG AAGAAAATAA AAACTCGGGA ACATCAGCCT TGGAAGTGCT TCGAAGGGCA 
ACCATCAAAA GGAGCCGGAC GGAGGCCATG ACCCGAGACT CCAGCGATGA GCACTGCGTT 
GATATCTCTT CAGTGGGCAC ACCTCTTGCC AGAGCCAGTA TCAAGAGCAC AAAAGTAGAT 
GGGGCCTCCT ATTTCCGGCA CAAGGAACGG CTTCTGCGCA TCTCTATCCG CCACATGGTC 
AAGTCCCAAG TCTTTTACTG GATCGTCCTG AGTGTTGTGG CTCTTAATAC TGCCTGTGTG 
GCCATTGTTC ATCACAACCA GCCACAGTGG CTCACTCACC TCCTCTACTA TGCAGAGTTT 
TTGTTTCTGG GACTCTTCCT GTTGGAGATG TCCCTGAAGA TGTATGGCAT GGGGCCACGC 
CTCTATTTTC ACTCATCCTT CAACTGCTTT GATTTTGGGG TCACAGTGGG CAGCATCTTT 
GAGGTTGTCT GGGCAATCTT CAGACCTGGC ACATCTTTTG GAATCAGTGT CTTACGAGCT 
CTCCGGCTCC TAAGGATATT TAAAATAACC AAGTATTGGG CATCCCTCCG GAATCTGGTT 
GTCTCCTTGA TGAGCTCCAT GAAGTCTATC ATCAGCTTAC TCTTCCTTCT CTTCCTCTTC 
ATCGTTGTCT TTGCTCTCCT GGGAATGCAG CTCTTTGGAG GCAGGTTTAA CTTTAATGAT 
GGGACTCCTT CAGCTAATTT TGACACCTTC CCTGCAGCTA TCATGACAGT GTTCCAGATC 
CTGACAGGGG AAGACTGGAA TGAGGTGATG TACAATGGCA TTCGCTCCCA GGGTGGGGTC 
AGCTCAGGCA TGTGGTCTGC CATCTACTTC ATTGTGCTCA CCTTGTTTGG AAACTACACG 
CTGCTAAATG TATTCTTGGC CATCGCTGTG GACAATCTGG CCAATGCCCA GGAACTGACC 
AAGGATGAGC AGGAGGAAGA AGAAGCCTTC AACCAGAAAC ATGCACTGCA GAAGGCCAAG 
GAGGTCAGCC CGATGTCTGC TCCCAACATG CCTTCCATTG AAAGAGACAG AAGGAGAAGA 
CACCACATGT CGATGTGGGA GCCACGCAGC AGCCACCTGA GGGAGCGGAG GCGCCGGCAC 
CACATGTCTG TGTGGGAACA ACGCACCAGC CAGCTGAGGA GGCACATGCA AATGTCTAGC 
CAAGAAGCCC TCAACAAAGA GGAGGCCCCG CCAATGAACC CCCTCAATCC ACTCAACCCA 
CTGAGTCCTC TCAACCCCCT CAATGCTCAC CCCAGCCTTT ATCGGAGGCC CAGGCCCATT 
GAGGGCCTAG CCCTAGGTCT GGGTCTTGAA AAGTGTGAAG AAGAACGCAT CAGTCGTGGG 
GGCTCCCTCA AGGGGGACAT AGGGGGCCTG ACTAGCGCCC TGGACAACCA GAGGAGCCCC 
TTGTCCTTGG GCAAACGGGA GCCACCGTGG CTGCCTAGAT CCTGTCATGG AAACTGTGAC 
CCAATCCAGC AGGAGGCTGG GGGAGGAGAG ACTGTGGTGA CTTTTGAGGA CCGGGCCAGG 
CACAGGCAGA GTCAGAGGCG AAGCCGGCAT CGCCGAGTCA GGACTGAGGG CAAGGACTCT 
GCCTCTGCAT CCCGAAGCAG GTCTGCCAGC CAGGAGCGGA GTCTGGATGA AGGGGTGTCC 
GTTGAGGGGG AGAAGGAACA TGAGCCTCAC AGCAGCCACA GGAGCAAAGA GCCGACCATC 
CACGAAGAGG AGAGAACCCA GGACTTAAGG AGGACCAACA GTCTGATGGT CCCCAGGGGC 
TCTGGACTGG TAGGGGCTCT TGATGAAGCA GAAACACCTC TAGTCCAGCC CCAACCTGAG 
CTGGAAGTGG GGAAGGATGC AGCTCTGACA GAGCAGGAGG CTGAAGGCAG CAGTGAACAA 
GCCCTTCTTG GGGACGTACA GCTGGATGTG GGCCGGGGCA TTAGCCAGAG TGAACCGGAT 
CTCTCCTGCA TGACAGCCAA CATGGACAAG GCTACCACGG AGAGCACCAG TGTCACGGTC 
GCCATCCCTG ATGTGGACCC CTTGGTGGAC TCGACAGTGG TGAACATTAG CAACAAGACG 
GATGGAGAAG CCAGTCCCTT GAAGGAGGCA GAGACTAAAG AGGAAGAAGA AGAGGTAGAG 
AAGAAGAAGA AGCAGAAGAA GGAGAAGCGA GAGACTGGAA AAGCCATGGT GCCCCATAGC 
TCTATGTTCA TCTTCAGCAC CACTAACCCG ATCCGCAGGG CCTGCCACTA CATTGTGAAC 
CTACGCTACT TTGAAATGTG CATTCTCTTG GTGATTGCGG CCAGCAGCAT TGCCCTGGCA 
GCCGAGGACC CTGTACTAAC TAACTCGGAG CGTAACAAAG TCCTGAGATA TTTCGACTAT 
GTTTTCACAG GAGTGTTTAC CTTTGAGATG GTTATAAAGA TGATAGACCA AGGCTTGATC 
CTGCAGGATG GATCATACTT CAGAGACCTC TGGAACATCC TGGACTTTGT AGTAGTGGTT 
GGTGCATTGG TAGCATTTGC TCTGGCAACC AACAAGGGGA GGGATATCAA GACTATCAAG 
TCTCTGAGGG TACTTCGAGT TCTGCGGCCA CTGAAAACCA TCAAGCGCTT GCCCAAGCTG 
AAGGCTGTGT TTGACTGCGT GGTGACCTCC TTGAAGAATG TCTTCAACAT ACTCATTGTC 
TACAAGCTCT TCATGTTCAT CTTTGCCGTC ATCGCTGTTC AGCTATTCAA AGGAAAGTTC 
TTTTATTGCA CTGACAGTTC CAAGGACACA GAGAAGGAGT GCATAGGCAA CTATGTAGAC 
CACGAGAAAA ACAAGATGGA GGTGAAGGGC CGGGAATGGA AGCGTCATGA ATTCCACTAT 
GACAACATCA TCTGGGCCCT GCTTACCCTC TTCACAGTCT CTACAGGGGA AGGATGGCCT 
CAGGTTCTGC AGCACTCTGT AGATGTGACA GAGGAGGACA GAGGCCCAAG TCGCAGCAAC 
CGCATGGAGA TGTCCATCTT TTATGTGGTC TACTTTGTGG TCTTCCCTTT CTTCTTTGTC 
AATATCTTCG TGGCTCTCAT TATCATCACC TTCCAGGAGC AAGGGGACAA GATGATGGAG 
GAGTGTAGCC TGGAGAAGAA TGAGCGGGCA TGCATCGACT TCGCCATTAG TGCCAAACCT 
CTCACCCGCT ACATGCCACA GAACAGGCAC ACCTTCCAGT ACCGTGTGTG GCACTTTGTA 
GTCTCCCCAT CCTTTGAGTA TACCATCATG GCCATGATTG CCTTGAACAC TGTCGTGCTG 
ATGATGAAGT ACTACTCTGC TCCTTGCACC TATGAGCTCG CCTTAAAGTA CCTGAACATC 
GCCTTCACTA TGGTGTTTTC CCTGGAGTGT GTCCTAAAGG TCATTGCTTT CGGCTTCCTG 
AACTATTTCC GAGATACCTG GAACATCTTT GACTTCATCA CGGTGATCGG CAGTATCACA 
GAAATTATCC TGACAGATAG CAAGCTGGTG AACACCAGTG GCTTCAATAT GAGCTTTCTG 
AAGCTCTTCC GAGCTGCACG GCTCATAAAG CTTCTGAGAC AGGGCTACAC CATCCGCATC 
TTACTGTGGA CCTTCGTGCA GTCCTTTAAG GCTCTTCCCT ACGTCTGCCT TCTCATTGCC 
ATGCTTTTCT TCATCTACGC CATCATTGGG ATGCAGGTGT TTGGAAACAT AAAGTTAGAT 
GAGGAGAGTC ACATCAACCG GCACAACAAC TTCCGGAGTT TCTTTGGATC CCTCATGCTG 
CTTTTCAGGA GTGCCACGGG GGAGGCTTGG CAGGAGATTA TGCTGTCCTG TCTCGGTGAG 
AAGGGTTGTG AGCCTGACAC CACTGCACCC TCAGGGCAGA ATGAGAGTGA GCGCTGTGGC 
ACTGACCTGG CCTACGTTTA TTTTGTCTCC TTCATCTTCT TCTGCTCCTT CCTGATGCTC 
AACCTGTTCG TGGCTGTCAT CATGGACAAC TTTGAGTACC TGACTCGAGA TTCCTCCATC 
CTGGGGCCTC ACCACTTAGA TGAGTTTGTC CGTGTCTGGG CAGAATATGA CAGAGCAGCA 
TGCGGCAGGA TCCCGTATAA GGATATGTAC AAATTAGTGC GGGTGATCTC GCCTCCTCTG 
GGCCTGGGAG AGAACTGCCC CTACAGGGTG GCTTGCAAGA GGCTGGTCCT GATGAACATG 
CCAGTGGCTG AGGACATGAC AGTCCACTTC ACCTCCACAC TTATGGCTCT GATCCGGACA 
GCTCTGGACA TTAAAATCGC CAAAGGTGGT GCAGACAGAC AGCAGCTAGA CTCGGAGCTG 
CAAAAAGAGA CCCTGGCCAT TTGGCCTCAC CTGTCCCAAA AGATGTTGGA TCTGCTTGTG 
CCCATGCCCA AAGCCTCTGA CCTGACTGTG GGCAAGATCT ATGCAGCAAT GATGATCATG 
GACTACTATA AACAGAGTAA GGTAAAGAAA CAGAGACAGC AGCTGGAAGA ACAGAAAAAT 
GCACCCATGT TCCAGCGCAT GGAGCCCTCA TCACTGCCTC AGGAGATCAT TGCTAATGCC 
AAAGCCCTGC CTTATCTCCA GCAGGACCCT GTTTCTGGCC TGAGTGGTCG GAGTGGATAC 
CCTTCAATGA GTCCACTCTC CCCTCAAGAA ATATTCCAGT TGGCTTGTAT GGACCCAGCG 
GATGATGGAC AATTCCAGGA ACAACAATCT CTGGTGAATG CACAAGAGCC AGAGGTTAGT 
GAATTAAAAA GTGTGCAGTC CTCTAACCAT GGCATCTACC TTCCTCCGGA CACCCAGGAG 
CATGCGGGAT CTGGGAGGGC ATCCTCTATG CCACGTCTGA CTATGGATCC CCAGGTGGTA 
ACAGACCCTA GCTCTATGCG ACGTTCATTT TCTACAATTC GGGATAAGCG TTCAAATTCC 
TCCTGGTTGG AGGAGTTCTC CATGGAACGC AGCAGTGAAA ACACTTACAA GTCGCGGCGC 
AGAAGTTACC ATTCCTCCCT GAGGCTGTCA GCACACCGCC TGAATTCTGA CTCTGGTCAC 
AAGTCTGACA CTCACCGATC AGGAGGCAGG GAGAGAGGCC GATCCAAAGA ACGAAAGCAC 
CTCCTCTCTC CAGATGTCTC CCGCTGCAAT TCTGAAGAGC GAGGGACCCA GGCCGACTGG 
GAGTCCCCAG AACGTCGCCA GTCCAGGTCA CCCAGTGAAG GAAGGTCACA GACCCCCAAT 
AGACAGGGTA CCGGTTCCCT AAGTGAGAGC TCCATCCCCT CTATTTCCGA CACCAGCACC 
CCAAGACGAA GCCGTCGCCA GCTCCCACCT GTGCCACCAA AGCCTCGTCC CCTCCTCTCC 
TACAGCTCCC TGATGAGACA CACTGGCGGC ATCTCTCCAC CTCCTGATGG AAGTGAGGGT 
GGATCCCCAC TGGCCTCTCA AGCTCTGGAG AGCAACAGCG CTTGCCTGAC CGAGTCTTCC 
AACTCCTTGC ACCCCCAGCA GGGCCAGCAT CCTTCCCCAC AGCACTACAT CTCCGAGCCC 
TATCTGGCCC TCCATGAAGA CTCCCACGCC TCAGACTGTG GCGAGGAGGA GACACTCACC 
TTTGAGGCAG CCGTAGCCAC TAGCTTGGGC CGGTCCAATA CCATTGGCTC TGCGCCACCC 
CTACGGCACA GCTGGCAGAT GCCTAATGGG CACTATCGGC GGCGGAGGCG TGGGGGGCCT 
GGGCCTGGCA TGATGTGTGG AGCTGTCAGT GACCTCCTGA GTGACACGGA AGAAGACGAT 
AAGTGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cacna1e ( XM_017312660.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017168149.1 Mus musculus calcium channel, voltage-dependent, R type, alpha 1E subunit (Cacna1e), transcript variant X9, mRNA.

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CloneID	OMu67264
Clone ID Related Accession (Same CDS sequence)	XM_017312660.1
Accession Version	XM_017312660.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7344bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	voltage-dependent R-type calcium channel subunit alpha-1E isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGCCCG AGATGCGTGG ACTGAGCCCA GGCCGAAGGC TGCCCGAGCC CACCCTGTGC 
CCGGCTACAG GAGGGAGCCC TGAGCCCGGG GCTGCTGGCA GGCGCCGCCA CGTCCTGGCT 
CTGCCTGATG AGGAGGCATG GCGGCCGCAG CAGTGCGCCC TGCTCGAGGC CAATGCCTCC 
GACGAGGTGG GCATGTTGCC TGCTTCCCCG CGCTCCGCGG TGGCCGGCTT CGACAACTTC 
TTCCCGGTGC AGGAAGGCGA GGGCCCGGGC TGGGAGGGCA GCACCACGCT GGATGCAGGC 
TCCAGCCCCT TCCTTCCTGT GAGCCCAGAG GTCATGAAGC GGAGGCGAGG GGGGCTCATC 
GAGCAGCGGG ACATCATCAA GGCCCACGAG GCACACAAGA TGCAGAGCAC CCCGCAGGCC 
CGGCGCAAGG AGTGGGAGAT GGCTCGCTTC GGGGAGGCGG TGGTCGTTGG CAGGCCAGGC 
TCAGGCGATG GAGACTCGGA CCAGAGCAGG AACCGACAAG GAACCCCCGT CCCGGCCTCG 
GGGCCGGCGG CCGCCTACAA GCAGTCAAAA GCGCAGAGGG CGCGGACTAT GGCTTTGTAC 
AACCCCATAC CAGTCCGGCA GAACTGTTTC ACGGTCAACA GATCCCTGTT CATCTTCGGA 
GAAGATAACA TTGTCAGGAA ATACGCCAAG AAGCTCATCG ATTGGCCGCC ATTTGAGTAC 
ATGATCCTGG CCACCATCAT TGCCAACTGC ATCGTCTTGG CCCTGGAGCA GCATCTTCCT 
GAGGATGACA AGACCCCAAT GTCTCGAAGA CTGGAGAAGA CAGAACCATA TTTCATTGGG 
ATCTTCTGCT TTGAAGCTGG GATCAAAATT GTGGCTCTAG GGTTCATCTT CCATAAGGGT 
TCATACCTCC GTAATGGCTG GAATGTCATG GACTTCATCG TGGTCCTTAG TGGCATCCTG 
GCCACTGCGG GAACCCACTT CAACACCCAC GTGGACCTGC GGACCCTCCG GGCTGTGCGT 
GTCCTGAGGC CTTTAAAGCT GGTATCAGGA ATACCTAGCC TGCAGATCGT GCTGAAGTCT 
ATCATGAAGG CCATGGTGCC TCTTCTGCAG ATTGGCCTTC TGCTCTTCTT TGCCATCCTC 
ATGTTTGCTA TCATTGGCTT GGAGTTCTAC AGTGGCAAGT TGCATCGAGC ATGCTTCATG 
AACAATTCAG GTATTCTAGA GGGGTTTGAT CCTCCTCACC CGTGTGGTGT GCAAGGCTGC 
CCGGCTGGTT ATGAATGTAA GGACTGGATC GGCCCCAATG ACGGGATCAC CCAGTTTGAC 
AACATCCTTT TTGCCGTGCT GACTGTCTTC CAATGCATCA CTATGGAAGG GTGGACCACT 
GTGCTGTACA ATACCAATGA TGCCTTAGGA GCCACCTGGA ACTGGCTGTA CTTCATCCCT 
CTCATCATCA TCGGATCCTT CTTTGTTCTC AACCTCGTCC TGGGAGTGCT TTCCGGGGAA 
TTTGCCAAAG AAAGAGAGAG AGTGGAGAAC CGAAGAGCGT TCATGAAGCT CCGGCGCCAG 
CAACAGATTG AGCGAGAATT GAACGGCTAC CGTGCCTGGA TAGACAAAGC AGAGGAAGTC 
ATGCTTGCTG AAGAAAATAA AAACTCGGGA ACATCAGCCT TGGAAGTGCT TCGAAGGGCA 
ACCATCAAAA GGAGCCGGAC GGAGGCCATG ACCCGAGACT CCAGCGATGA GCACTGCGTT 
GATATCTCTT CAGTGGGCAC ACCTCTTGCC AGAGCCAGTA TCAAGAGCAC AAAAGTAGAT 
GGGGCCTCCT ATTTCCGGCA CAAGGAACGG CTTCTGCGCA TCTCTATCCG CCACATGGTC 
AAGTCCCAAG TCTTTTACTG GATCGTCCTG AGTGTTGTGG CTCTTAATAC TGCCTGTGTG 
GCCATTGTTC ATCACAACCA GCCACAGTGG CTCACTCACC TCCTCTACTA TGCAGAGTTT 
TTGTTTCTGG GACTCTTCCT GTTGGAGATG TCCCTGAAGA TGTATGGCAT GGGGCCACGC 
CTCTATTTTC ACTCATCCTT CAACTGCTTT GATTTTGGGG TCACAGTGGG CAGCATCTTT 
GAGGTTGTCT GGGCAATCTT CAGACCTGGC ACATCTTTTG GAATCAGTGT CTTACGAGCT 
CTCCGGCTCC TAAGGATATT TAAAATAACC AAGTATTGGG CATCCCTCCG GAATCTGGTT 
GTCTCCTTGA TGAGCTCCAT GAAGTCTATC ATCAGCTTAC TCTTCCTTCT CTTCCTCTTC 
ATCGTTGTCT TTGCTCTCCT GGGAATGCAG CTCTTTGGAG GCAGGTTTAA CTTTAATGAT 
GGGACTCCTT CAGCTAATTT TGACACCTTC CCTGCAGCTA TCATGACAGT GTTCCAGATC 
CTGACAGGGG AAGACTGGAA TGAGGTGATG TACAATGGCA TTCGCTCCCA GGGTGGGGTC 
AGCTCAGGCA TGTGGTCTGC CATCTACTTC ATTGTGCTCA CCTTGTTTGG AAACTACACG 
CTGCTAAATG TATTCTTGGC CATCGCTGTG GACAATCTGG CCAATGCCCA GGAACTGACC 
AAGGATGAGC AGGAGGAAGA AGAAGCCTTC AACCAGAAAC ATGCACTGCA GAAGGCCAAG 
GAGGTCAGCC CGATGTCTGC TCCCAACATG CCTTCCATTG AGAGGGAGCG GAGGCGCCGG 
CACCACATGT CTGTGTGGGA ACAACGCACC AGCCAGCTGA GGAGGCACAT GCAAATGTCT 
AGCCAAGAAG CCCTCAACAA AGAGGAGGCC CCGCCAATGA ACCCCCTCAA TCCACTCAAC 
CCACTGAGTC CTCTCAACCC CCTCAATGCT CACCCCAGCC TTTATCGGAG GCCCAGGCCC 
ATTGAGGGCC TAGCCCTAGG TCTGGGTCTT GAAAAGTGTG AAGAAGAACG CATCAGTCGT 
GGGGGCTCCC TCAAGGGGGA CATAGGGGGC CTGACTAGCG CCCTGGACAA CCAGAGGAGC 
CCCTTGTCCT TGGGCAAACG GGAGCCACCG TGGCTGCCTA GATCCTGTCA TGGAAACTGT 
GACCCAATCC AGCAGGAGGC TGGGGGAGGA GAGACTGTGG TGACTTTTGA GGACCGGGCC 
AGGCACAGGC AGAGTCAGAG GCGAAGCCGG CATCGCCGAG TCAGGACTGA GGGCAAGGAC 
TCTGCCTCTG CATCCCGAAG CAGGTCTGCC AGCCAGGAGC GGAGTCTGGA TGAAGGGGTG 
TCCGTTGAGG GGGAGAAGGA ACATGAGCCT CACAGCAGCC ACAGGAGCAA AGAGCCGACC 
ATCCACGAAG AGGAGAGAAC CCAGGACTTA AGGAGGACCA ACAGTCTGAT GGTCCCCAGG 
GGCTCTGGAC TGGTAGGGGC TCTTGATGAA GCAGAAACAC CTCTAGTCCA GCCCCAACCT 
GAGCTGGAAG TGGGGAAGGA TGCAGCTCTG ACAGAGCAGG AGGCTGAAGG CAGCAGTGAA 
CAAGCCCTTC TTGGGGACGT ACAGCTGGAT GTGGGCCGGG GCATTAGCCA GAGTGAACCG 
GATCTCTCCT GCATGACAGC CAACATGGAC AAGGCTACCA CGGAGAGCAC CAGTGTCACG 
GTCGCCATCC CTGATGTGGA CCCCTTGGTG GACTCGACAG TGGTGAACAT TAGCAACAAG 
ACGGATGGAG AAGCCAGTCC CTTGAAGGAG GCAGAGACTA AAGAGGAAGA AGAAGAGGTA 
GAGAAGAAGA AGAAGCAGAA GAAGGAGAAG CGAGAGACTG GAAAAGCCAT GGTGCCCCAT 
AGCTCTATGT TCATCTTCAG CACCACTAAC CCGATCCGCA GGGCCTGCCA CTACATTGTG 
AACCTACGCT ACTTTGAAAT GTGCATTCTC TTGGTGATTG CGGCCAGCAG CATTGCCCTG 
GCAGCCGAGG ACCCTGTACT AACTAACTCG GAGCGTAACA AAGTCCTGAG ATATTTCGAC 
TATGTTTTCA CAGGAGTGTT TACCTTTGAG ATGGTTATAA AGATGATAGA CCAAGGCTTG 
ATCCTGCAGG ATGGATCATA CTTCAGAGAC CTCTGGAACA TCCTGGACTT TGTAGTAGTG 
GTTGGTGCAT TGGTAGCATT TGCTCTGGCG AACGCTTTGG GAACCAACAA GGGGAGGGAT 
ATCAAGACTA TCAAGTCTCT GAGGGTACTT CGAGTTCTGC GGCCACTGAA AACCATCAAG 
CGCTTGCCCA AGCTGAAGGC TGTGTTTGAC TGCGTGGTGA CCTCCTTGAA GAATGTCTTC 
AACATACTCA TTGTCTACAA GCTCTTCATG TTCATCTTTG CCGTCATCGC TGTTCAGCTA 
TTCAAAGGAA AGTTCTTTTA TTGCACTGAC AGTTCCAAGG ACACAGAGAA GGAGTGCATA 
GGCAACTATG TAGACCACGA GAAAAACAAG ATGGAGGTGA AGGGCCGGGA ATGGAAGCGT 
CATGAATTCC ACTATGACAA CATCATCTGG GCCCTGCTTA CCCTCTTCAC AGTCTCTACA 
GGGGAAGGAT GGCCTCAGGT TCTGCAGCAC TCTGTAGATG TGACAGAGGA GGACAGAGGC 
CCAAGTCGCA GCAACCGCAT GGAGATGTCC ATCTTTTATG TGGTCTACTT TGTGGTCTTC 
CCTTTCTTCT TTGTCAATAT CTTCGTGGCT CTCATTATCA TCACCTTCCA GGAGCAAGGG 
GACAAGATGA TGGAGGAGTG TAGCCTGGAG AAGAATGAGC GGGCATGCAT CGACTTCGCC 
ATTAGTGCCA AACCTCTCAC CCGCTACATG CCACAGAACA GGCACACCTT CCAGTACCGT 
GTGTGGCACT TTGTAGTCTC CCCATCCTTT GAGTATACCA TCATGGCCAT GATTGCCTTG 
AACACTGTCG TGCTGATGAT GAAGTACTAC TCTGCTCCTT GCACCTATGA GCTCGCCTTA 
AAGTACCTGA ACATCGCCTT CACTATGGTG TTTTCCCTGG AGTGTGTCCT AAAGGTCATT 
GCTTTCGGCT TCCTGAACTA TTTCCGAGAT ACCTGGAACA TCTTTGACTT CATCACGGTG 
ATCGGCAGTA TCACAGAAAT TATCCTGACA GATAGCAAGC TGGTGAACAC CAGTGGCTTC 
AATATGAGCT TTCTGAAGCT CTTCCGAGCT GCACGGCTCA TAAAGCTTCT GAGACAGGGC 
TACACCATCC GCATCTTACT GTGGACCTTC GTGCAGTCCT TTAAGGCTCT TCCCTACGTC 
TGCCTTCTCA TTGCCATGCT TTTCTTCATC TACGCCATCA TTGGGATGCA GGTGTTTGGA 
AACATAAAGT TAGATGAGGA GAGTCACATC AACCGGCACA ACAACTTCCG GAGTTTCTTT 
GGATCCCTCA TGCTGCTTTT CAGGAGTGCC ACGGGGGAGG CTTGGCAGGA GATTATGCTG 
TCCTGTCTCG GTGAGAAGGG TTGTGAGCCT GACACCACTG CACCCTCAGG GCAGAATGAG 
AGTGAGCGCT GTGGCACTGA CCTGGCCTAC GTTTATTTTG TCTCCTTCAT CTTCTTCTGC 
TCCTTCCTGA TGCTCAACCT GTTCGTGGCT GTCATCATGG ACAACTTTGA GTACCTGACT 
CGAGATTCCT CCATCCTGGG GCCTCACCAC TTAGATGAGT TTGTCCGTGT CTGGGCAGAA 
TATGACAGAG CAGCATGTGG CCGCATCCAT TACACTGAGA TGTATGAAAT GCTGACTCTC 
ATGTCACCAC CGCTAGGCCT CGGCAAGAGA TGTCCCTCCA AAGTGGCCTA TAAGAGGCTG 
GTCCTGATGA ACATGCCAGT GGCTGAGGAC ATGACAGTCC ACTTCACCTC CACACTTATG 
GCTCTGATCC GGACAGCTCT GGACATTAAA ATCGCCAAAG GTGGTGCAGA CAGACAGCAG 
CTAGACTCGG AGCTGCAAAA AGAGACCCTG GCCATTTGGC CTCACCTGTC CCAAAAGATG 
TTGGATCTGC TTGTGCCCAT GCCCAAAGCC TCTGACCTGA CTGTGGGCAA GATCTATGCA 
GCAATGATGA TCATGGACTA CTATAAACAG AGTAAGGTAA AGAAACAGAG ACAGCAGCTG 
GAAGAACAGA AAAATGCACC CATGTTCCAG CGCATGGAGC CCTCATCACT GCCTCAGGAG 
ATCATTGCTA ATGCCAAAGC CCTGCCTTAT CTCCAGCAGG ACCCTGTTTC TGGCCTGAGT 
GGTCGGAGTG GATACCCTTC AATGAGTCCA CTCTCCCCTC AAGAAATATT CCAGTTGGCT 
TGTATGGACC CAGCGGATGA TGGACAATTC CAGGAACAAC AATCTCTGGT GAATGCACAA 
GAGCCAGAGG TTAGTGAATT AAAAAGTGTG CAGTCCTCTA ACCATGGCAT CTACCTTCCT 
CCGGACACCC AGGAGCATGC GGGATCTGGG AGGGCATCCT CTATGCCACG TCTGACTATG 
GATCCCCAGG TGGTAACAGA CCCTAGCTCT ATGCGACGTT CATTTTCTAC AATTCGGGAT 
AAGCGTTCAA ATTCCTCCTG GTTGGAGGAG TTCTCCATGG AACGCAGCAG TGAAAACACT 
TACAAGTCGC GGCGCAGAAG TTACCATTCC TCCCTGAGGC TGTCAGCACA CCGCCTGAAT 
TCTGACTCTG GTCACAAGTC TGACACTCAC CGATCAGGAG GCAGGGAGAG AGGCCGATCC 
AAAGAACGAA AGCACCTCCT CTCTCCAGAT GTCTCCCGCT GCAATTCTGA AGAGCGAGGG 
ACCCAGGCCG ACTGGGAGTC CCCAGAACGT CGCCAGTCCA GGTCACCCAG TGAAGGAAGG 
TCACAGACCC CCAATAGACA GGGTACCGGT TCCCTAAGTG AGAGCTCCAT CCCCTCTATT 
TCCGACACCA GCACCCCAAG ACGAAGCCGT CGCCAGCTCC CACCTGTGCC ACCAAAGCCT 
CGTCCCCTCC TCTCCTACAG CTCCCTGATG AGACACACTG GCGGCATCTC TCCACCTCCT 
GATGGAAGTG AGGGTGGATC CCCACTGGCC TCTCAAGCTC TGGAGAGCAA CAGCGCTTGC 
CTGACCGAGT CTTCCAACTC CTTGCACCCC CAGCAGGGCC AGCATCCTTC CCCACAGCAC 
TACATCTCCG AGCCCTATCT GGCCCTCCAT GAAGACTCCC ACGCCTCAGA CTGTGGCGAG 
GAGGAGACAC TCACCTTTGA GGCAGCCGTA GCCACTAGCT TGGGCCGGTC CAATACCATT 
GGCTCTGCGC CACCCCTACG GCACAGCTGG CAGATGCCTA ATGGGCACTA TCGGCGGCGG 
AGGCGTGGGG GGCCTGGGCC TGGCATGATG TGTGGAGCTG TCAGTGACCT CCTGAGTGAC 
ACGGAAGAAG ACGATAAGTG CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017168149.1
CDS	594..7937
Translation	MVPEMRGLSPGRRLPEPTLCPATGGSPEPGAAGRRRHVLALPDEEAWRPQQCALLEANASDEVGMLPASPRSAVAGFDNFFPVQEGEGPGWEGSTTLDAGSSPFLPVSPEVMKRRRGGLIEQRDIIKAHEAHKMQSTPQARRKEWEMARFGEAVVVGRPGSGDGDSDQSRNRQGTPVPASGPAAAYKQSKAQRARTMALYNPIPVRQNCFTVNRSLFIFGEDNIVRKYAKKLIDWPPFEYMILATIIANCIVLALEQHLPEDDKTPMSRRLEKTEPYFIGIFCFEAGIKIVALGFIFHKGSYLRNGWNVMDFIVVLSGILATAGTHFNTHVDLRTLRAVRVLRPLKLVSGIPSLQIVLKSIMKAMVPLLQIGLLLFFAILMFAIIGLEFYSGKLHRACFMNNSGILEGFDPPHPCGVQGCPAGYECKDWIGPNDGITQFDNILFAVLTVFQCITMEGWTTVLYNTNDALGATWNWLYFIPLIIIGSFFVLNLVLGVLSGEFAKERERVENRRAFMKLRRQQQIERELNGYRAWIDKAEEVMLAEENKNSGTSALEVLRRATIKRSRTEAMTRDSSDEHCVDISSVGTPLARASIKSTKVDGASYFRHKERLLRISIRHMVKSQVFYWIVLSVVALNTACVAIVHHNQPQWLTHLLYYAEFLFLGLFLLEMSLKMYGMGPRLYFHSSFNCFDFGVTVGSIFEVVWAIFRPGTSFGISVLRALRLLRIFKITKYWASLRNLVVSLMSSMKSIISLLFLLFLFIVVFALLGMQLFGGRFNFNDGTPSANFDTFPAAIMTVFQILTGEDWNEVMYNGIRSQGGVSSGMWSAIYFIVLTLFGNYTLLNVFLAIAVDNLANAQELTKDEQEEEEAFNQKHALQKAKEVSPMSAPNMPSIERERRRRHHMSVWEQRTSQLRRHMQMSSQEALNKEEAPPMNPLNPLNPLSPLNPLNAHPSLYRRPRPIEGLALGLGLEKCEEERISRGGSLKGDIGGLTSALDNQRSPLSLGKREPPWLPRSCHGNCDPIQQEAGGGETVVTFEDRARHRQSQRRSRHRRVRTEGKDSASASRSRSASQERSLDEGVSVEGEKEHEPHSSHRSKEPTIHEEERTQDLRRTNSLMVPRGSGLVGALDEAETPLVQPQPELEVGKDAALTEQEAEGSSEQALLGDVQLDVGRGISQSEPDLSCMTANMDKATTESTSVTVAIPDVDPLVDSTVVNISNKTDGEASPLKEAETKEEEEEVEKKKKQKKEKRETGKAMVPHSSMFIFSTTNPIRRACHYIVNLRYFEMCILLVIAASSIALAAEDPVLTNSERNKVLRYFDYVFTGVFTFEMVIKMIDQGLILQDGSYFRDLWNILDFVVVVGALVAFALANALGTNKGRDIKTIKSLRVLRVLRPLKTIKRLPKLKAVFDCVVTSLKNVFNILIVYKLFMFIFAVIAVQLFKGKFFYCTDSSKDTEKECIGNYVDHEKNKMEVKGREWKRHEFHYDNIIWALLTLFTVSTGEGWPQVLQHSVDVTEEDRGPSRSNRMEMSIFYVVYFVVFPFFFVNIFVALIIITFQEQGDKMMEECSLEKNERACIDFAISAKPLTRYMPQNRHTFQYRVWHFVVSPSFEYTIMAMIALNTVVLMMKYYSAPCTYELALKYLNIAFTMVFSLECVLKVIAFGFLNYFRDTWNIFDFITVIGSITEIILTDSKLVNTSGFNMSFLKLFRAARLIKLLRQGYTIRILLWTFVQSFKALPYVCLLIAMLFFIYAIIGMQVFGNIKLDEESHINRHNNFRSFFGSLMLLFRSATGEAWQEIMLSCLGEKGCEPDTTAPSGQNESERCGTDLAYVYFVSFIFFCSFLMLNLFVAVIMDNFEYLTRDSSILGPHHLDEFVRVWAEYDRAACGRIHYTEMYEMLTLMSPPLGLGKRCPSKVAYKRLVLMNMPVAEDMTVHFTSTLMALIRTALDIKIAKGGADRQQLDSELQKETLAIWPHLSQKMLDLLVPMPKASDLTVGKIYAAMMIMDYYKQSKVKKQRQQLEEQKNAPMFQRMEPSSLPQEIIANAKALPYLQQDPVSGLSGRSGYPSMSPLSPQEIFQLACMDPADDGQFQEQQSLVNAQEPEVSELKSVQSSNHGIYLPPDTQEHAGSGRASSMPRLTMDPQVVTDPSSMRRSFSTIRDKRSNSSWLEEFSMERSSENTYKSRRRSYHSSLRLSAHRLNSDSGHKSDTHRSGGRERGRSKERKHLLSPDVSRCNSEERGTQADWESPERRQSRSPSEGRSQTPNRQGTGSLSESSIPSISDTSTPRRSRRQLPPVPPKPRPLLSYSSLMRHTGGISPPPDGSEGGSPLASQALESNSACLTESSNSLHPQQGQHPSPQHYISEPYLALHEDSHASDCGEEETLTFEAAVATSLGRSNTIGSAPPLRHSWQMPNGHYRRRRRGGPGPGMMCGAVSDLLSDTEEDDKC

Target ORF information:

RefSeq Version	XM_017312660.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus calcium channel, voltage-dependent, R type, alpha 1E subunit (Cacna1e), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017312660.1

ORF Insert Sequence:

ATGGTGCCCG AGATGCGTGG ACTGAGCCCA GGCCGAAGGC TGCCCGAGCC CACCCTGTGC 
CCGGCTACAG GAGGGAGCCC TGAGCCCGGG GCTGCTGGCA GGCGCCGCCA CGTCCTGGCT 
CTGCCTGATG AGGAGGCATG GCGGCCGCAG CAGTGCGCCC TGCTCGAGGC CAATGCCTCC 
GACGAGGTGG GCATGTTGCC TGCTTCCCCG CGCTCCGCGG TGGCCGGCTT CGACAACTTC 
TTCCCGGTGC AGGAAGGCGA GGGCCCGGGC TGGGAGGGCA GCACCACGCT GGATGCAGGC 
TCCAGCCCCT TCCTTCCTGT GAGCCCAGAG GTCATGAAGC GGAGGCGAGG GGGGCTCATC 
GAGCAGCGGG ACATCATCAA GGCCCACGAG GCACACAAGA TGCAGAGCAC CCCGCAGGCC 
CGGCGCAAGG AGTGGGAGAT GGCTCGCTTC GGGGAGGCGG TGGTCGTTGG CAGGCCAGGC 
TCAGGCGATG GAGACTCGGA CCAGAGCAGG AACCGACAAG GAACCCCCGT CCCGGCCTCG 
GGGCCGGCGG CCGCCTACAA GCAGTCAAAA GCGCAGAGGG CGCGGACTAT GGCTTTGTAC 
AACCCCATAC CAGTCCGGCA GAACTGTTTC ACGGTCAACA GATCCCTGTT CATCTTCGGA 
GAAGATAACA TTGTCAGGAA ATACGCCAAG AAGCTCATCG ATTGGCCGCC ATTTGAGTAC 
ATGATCCTGG CCACCATCAT TGCCAACTGC ATCGTCTTGG CCCTGGAGCA GCATCTTCCT 
GAGGATGACA AGACCCCAAT GTCTCGAAGA CTGGAGAAGA CAGAACCATA TTTCATTGGG 
ATCTTCTGCT TTGAAGCTGG GATCAAAATT GTGGCTCTAG GGTTCATCTT CCATAAGGGT 
TCATACCTCC GTAATGGCTG GAATGTCATG GACTTCATCG TGGTCCTTAG TGGCATCCTG 
GCCACTGCGG GAACCCACTT CAACACCCAC GTGGACCTGC GGACCCTCCG GGCTGTGCGT 
GTCCTGAGGC CTTTAAAGCT GGTATCAGGA ATACCTAGCC TGCAGATCGT GCTGAAGTCT 
ATCATGAAGG CCATGGTGCC TCTTCTGCAG ATTGGCCTTC TGCTCTTCTT TGCCATCCTC 
ATGTTTGCTA TCATTGGCTT GGAGTTCTAC AGTGGCAAGT TGCATCGAGC ATGCTTCATG 
AACAATTCAG GTATTCTAGA GGGGTTTGAT CCTCCTCACC CGTGTGGTGT GCAAGGCTGC 
CCGGCTGGTT ATGAATGTAA GGACTGGATC GGCCCCAATG ACGGGATCAC CCAGTTTGAC 
AACATCCTTT TTGCCGTGCT GACTGTCTTC CAATGCATCA CTATGGAAGG GTGGACCACT 
GTGCTGTACA ATACCAATGA TGCCTTAGGA GCCACCTGGA ACTGGCTGTA CTTCATCCCT 
CTCATCATCA TCGGATCCTT CTTTGTTCTC AACCTCGTCC TGGGAGTGCT TTCCGGGGAA 
TTTGCCAAAG AAAGAGAGAG AGTGGAGAAC CGAAGAGCGT TCATGAAGCT CCGGCGCCAG 
CAACAGATTG AGCGAGAATT GAACGGCTAC CGTGCCTGGA TAGACAAAGC AGAGGAAGTC 
ATGCTTGCTG AAGAAAATAA AAACTCGGGA ACATCAGCCT TGGAAGTGCT TCGAAGGGCA 
ACCATCAAAA GGAGCCGGAC GGAGGCCATG ACCCGAGACT CCAGCGATGA GCACTGCGTT 
GATATCTCTT CAGTGGGCAC ACCTCTTGCC AGAGCCAGTA TCAAGAGCAC AAAAGTAGAT 
GGGGCCTCCT ATTTCCGGCA CAAGGAACGG CTTCTGCGCA TCTCTATCCG CCACATGGTC 
AAGTCCCAAG TCTTTTACTG GATCGTCCTG AGTGTTGTGG CTCTTAATAC TGCCTGTGTG 
GCCATTGTTC ATCACAACCA GCCACAGTGG CTCACTCACC TCCTCTACTA TGCAGAGTTT 
TTGTTTCTGG GACTCTTCCT GTTGGAGATG TCCCTGAAGA TGTATGGCAT GGGGCCACGC 
CTCTATTTTC ACTCATCCTT CAACTGCTTT GATTTTGGGG TCACAGTGGG CAGCATCTTT 
GAGGTTGTCT GGGCAATCTT CAGACCTGGC ACATCTTTTG GAATCAGTGT CTTACGAGCT 
CTCCGGCTCC TAAGGATATT TAAAATAACC AAGTATTGGG CATCCCTCCG GAATCTGGTT 
GTCTCCTTGA TGAGCTCCAT GAAGTCTATC ATCAGCTTAC TCTTCCTTCT CTTCCTCTTC 
ATCGTTGTCT TTGCTCTCCT GGGAATGCAG CTCTTTGGAG GCAGGTTTAA CTTTAATGAT 
GGGACTCCTT CAGCTAATTT TGACACCTTC CCTGCAGCTA TCATGACAGT GTTCCAGATC 
CTGACAGGGG AAGACTGGAA TGAGGTGATG TACAATGGCA TTCGCTCCCA GGGTGGGGTC 
AGCTCAGGCA TGTGGTCTGC CATCTACTTC ATTGTGCTCA CCTTGTTTGG AAACTACACG 
CTGCTAAATG TATTCTTGGC CATCGCTGTG GACAATCTGG CCAATGCCCA GGAACTGACC 
AAGGATGAGC AGGAGGAAGA AGAAGCCTTC AACCAGAAAC ATGCACTGCA GAAGGCCAAG 
GAGGTCAGCC CGATGTCTGC TCCCAACATG CCTTCCATTG AGAGGGAGCG GAGGCGCCGG 
CACCACATGT CTGTGTGGGA ACAACGCACC AGCCAGCTGA GGAGGCACAT GCAAATGTCT 
AGCCAAGAAG CCCTCAACAA AGAGGAGGCC CCGCCAATGA ACCCCCTCAA TCCACTCAAC 
CCACTGAGTC CTCTCAACCC CCTCAATGCT CACCCCAGCC TTTATCGGAG GCCCAGGCCC 
ATTGAGGGCC TAGCCCTAGG TCTGGGTCTT GAAAAGTGTG AAGAAGAACG CATCAGTCGT 
GGGGGCTCCC TCAAGGGGGA CATAGGGGGC CTGACTAGCG CCCTGGACAA CCAGAGGAGC 
CCCTTGTCCT TGGGCAAACG GGAGCCACCG TGGCTGCCTA GATCCTGTCA TGGAAACTGT 
GACCCAATCC AGCAGGAGGC TGGGGGAGGA GAGACTGTGG TGACTTTTGA GGACCGGGCC 
AGGCACAGGC AGAGTCAGAG GCGAAGCCGG CATCGCCGAG TCAGGACTGA GGGCAAGGAC 
TCTGCCTCTG CATCCCGAAG CAGGTCTGCC AGCCAGGAGC GGAGTCTGGA TGAAGGGGTG 
TCCGTTGAGG GGGAGAAGGA ACATGAGCCT CACAGCAGCC ACAGGAGCAA AGAGCCGACC 
ATCCACGAAG AGGAGAGAAC CCAGGACTTA AGGAGGACCA ACAGTCTGAT GGTCCCCAGG 
GGCTCTGGAC TGGTAGGGGC TCTTGATGAA GCAGAAACAC CTCTAGTCCA GCCCCAACCT 
GAGCTGGAAG TGGGGAAGGA TGCAGCTCTG ACAGAGCAGG AGGCTGAAGG CAGCAGTGAA 
CAAGCCCTTC TTGGGGACGT ACAGCTGGAT GTGGGCCGGG GCATTAGCCA GAGTGAACCG 
GATCTCTCCT GCATGACAGC CAACATGGAC AAGGCTACCA CGGAGAGCAC CAGTGTCACG 
GTCGCCATCC CTGATGTGGA CCCCTTGGTG GACTCGACAG TGGTGAACAT TAGCAACAAG 
ACGGATGGAG AAGCCAGTCC CTTGAAGGAG GCAGAGACTA AAGAGGAAGA AGAAGAGGTA 
GAGAAGAAGA AGAAGCAGAA GAAGGAGAAG CGAGAGACTG GAAAAGCCAT GGTGCCCCAT 
AGCTCTATGT TCATCTTCAG CACCACTAAC CCGATCCGCA GGGCCTGCCA CTACATTGTG 
AACCTACGCT ACTTTGAAAT GTGCATTCTC TTGGTGATTG CGGCCAGCAG CATTGCCCTG 
GCAGCCGAGG ACCCTGTACT AACTAACTCG GAGCGTAACA AAGTCCTGAG ATATTTCGAC 
TATGTTTTCA CAGGAGTGTT TACCTTTGAG ATGGTTATAA AGATGATAGA CCAAGGCTTG 
ATCCTGCAGG ATGGATCATA CTTCAGAGAC CTCTGGAACA TCCTGGACTT TGTAGTAGTG 
GTTGGTGCAT TGGTAGCATT TGCTCTGGCG AACGCTTTGG GAACCAACAA GGGGAGGGAT 
ATCAAGACTA TCAAGTCTCT GAGGGTACTT CGAGTTCTGC GGCCACTGAA AACCATCAAG 
CGCTTGCCCA AGCTGAAGGC TGTGTTTGAC TGCGTGGTGA CCTCCTTGAA GAATGTCTTC 
AACATACTCA TTGTCTACAA GCTCTTCATG TTCATCTTTG CCGTCATCGC TGTTCAGCTA 
TTCAAAGGAA AGTTCTTTTA TTGCACTGAC AGTTCCAAGG ACACAGAGAA GGAGTGCATA 
GGCAACTATG TAGACCACGA GAAAAACAAG ATGGAGGTGA AGGGCCGGGA ATGGAAGCGT 
CATGAATTCC ACTATGACAA CATCATCTGG GCCCTGCTTA CCCTCTTCAC AGTCTCTACA 
GGGGAAGGAT GGCCTCAGGT TCTGCAGCAC TCTGTAGATG TGACAGAGGA GGACAGAGGC 
CCAAGTCGCA GCAACCGCAT GGAGATGTCC ATCTTTTATG TGGTCTACTT TGTGGTCTTC 
CCTTTCTTCT TTGTCAATAT CTTCGTGGCT CTCATTATCA TCACCTTCCA GGAGCAAGGG 
GACAAGATGA TGGAGGAGTG TAGCCTGGAG AAGAATGAGC GGGCATGCAT CGACTTCGCC 
ATTAGTGCCA AACCTCTCAC CCGCTACATG CCACAGAACA GGCACACCTT CCAGTACCGT 
GTGTGGCACT TTGTAGTCTC CCCATCCTTT GAGTATACCA TCATGGCCAT GATTGCCTTG 
AACACTGTCG TGCTGATGAT GAAGTACTAC TCTGCTCCTT GCACCTATGA GCTCGCCTTA 
AAGTACCTGA ACATCGCCTT CACTATGGTG TTTTCCCTGG AGTGTGTCCT AAAGGTCATT 
GCTTTCGGCT TCCTGAACTA TTTCCGAGAT ACCTGGAACA TCTTTGACTT CATCACGGTG 
ATCGGCAGTA TCACAGAAAT TATCCTGACA GATAGCAAGC TGGTGAACAC CAGTGGCTTC 
AATATGAGCT TTCTGAAGCT CTTCCGAGCT GCACGGCTCA TAAAGCTTCT GAGACAGGGC 
TACACCATCC GCATCTTACT GTGGACCTTC GTGCAGTCCT TTAAGGCTCT TCCCTACGTC 
TGCCTTCTCA TTGCCATGCT TTTCTTCATC TACGCCATCA TTGGGATGCA GGTGTTTGGA 
AACATAAAGT TAGATGAGGA GAGTCACATC AACCGGCACA ACAACTTCCG GAGTTTCTTT 
GGATCCCTCA TGCTGCTTTT CAGGAGTGCC ACGGGGGAGG CTTGGCAGGA GATTATGCTG 
TCCTGTCTCG GTGAGAAGGG TTGTGAGCCT GACACCACTG CACCCTCAGG GCAGAATGAG 
AGTGAGCGCT GTGGCACTGA CCTGGCCTAC GTTTATTTTG TCTCCTTCAT CTTCTTCTGC 
TCCTTCCTGA TGCTCAACCT GTTCGTGGCT GTCATCATGG ACAACTTTGA GTACCTGACT 
CGAGATTCCT CCATCCTGGG GCCTCACCAC TTAGATGAGT TTGTCCGTGT CTGGGCAGAA 
TATGACAGAG CAGCATGTGG CCGCATCCAT TACACTGAGA TGTATGAAAT GCTGACTCTC 
ATGTCACCAC CGCTAGGCCT CGGCAAGAGA TGTCCCTCCA AAGTGGCCTA TAAGAGGCTG 
GTCCTGATGA ACATGCCAGT GGCTGAGGAC ATGACAGTCC ACTTCACCTC CACACTTATG 
GCTCTGATCC GGACAGCTCT GGACATTAAA ATCGCCAAAG GTGGTGCAGA CAGACAGCAG 
CTAGACTCGG AGCTGCAAAA AGAGACCCTG GCCATTTGGC CTCACCTGTC CCAAAAGATG 
TTGGATCTGC TTGTGCCCAT GCCCAAAGCC TCTGACCTGA CTGTGGGCAA GATCTATGCA 
GCAATGATGA TCATGGACTA CTATAAACAG AGTAAGGTAA AGAAACAGAG ACAGCAGCTG 
GAAGAACAGA AAAATGCACC CATGTTCCAG CGCATGGAGC CCTCATCACT GCCTCAGGAG 
ATCATTGCTA ATGCCAAAGC CCTGCCTTAT CTCCAGCAGG ACCCTGTTTC TGGCCTGAGT 
GGTCGGAGTG GATACCCTTC AATGAGTCCA CTCTCCCCTC AAGAAATATT CCAGTTGGCT 
TGTATGGACC CAGCGGATGA TGGACAATTC CAGGAACAAC AATCTCTGGT GAATGCACAA 
GAGCCAGAGG TTAGTGAATT AAAAAGTGTG CAGTCCTCTA ACCATGGCAT CTACCTTCCT 
CCGGACACCC AGGAGCATGC GGGATCTGGG AGGGCATCCT CTATGCCACG TCTGACTATG 
GATCCCCAGG TGGTAACAGA CCCTAGCTCT ATGCGACGTT CATTTTCTAC AATTCGGGAT 
AAGCGTTCAA ATTCCTCCTG GTTGGAGGAG TTCTCCATGG AACGCAGCAG TGAAAACACT 
TACAAGTCGC GGCGCAGAAG TTACCATTCC TCCCTGAGGC TGTCAGCACA CCGCCTGAAT 
TCTGACTCTG GTCACAAGTC TGACACTCAC CGATCAGGAG GCAGGGAGAG AGGCCGATCC 
AAAGAACGAA AGCACCTCCT CTCTCCAGAT GTCTCCCGCT GCAATTCTGA AGAGCGAGGG 
ACCCAGGCCG ACTGGGAGTC CCCAGAACGT CGCCAGTCCA GGTCACCCAG TGAAGGAAGG 
TCACAGACCC CCAATAGACA GGGTACCGGT TCCCTAAGTG AGAGCTCCAT CCCCTCTATT 
TCCGACACCA GCACCCCAAG ACGAAGCCGT CGCCAGCTCC CACCTGTGCC ACCAAAGCCT 
CGTCCCCTCC TCTCCTACAG CTCCCTGATG AGACACACTG GCGGCATCTC TCCACCTCCT 
GATGGAAGTG AGGGTGGATC CCCACTGGCC TCTCAAGCTC TGGAGAGCAA CAGCGCTTGC 
CTGACCGAGT CTTCCAACTC CTTGCACCCC CAGCAGGGCC AGCATCCTTC CCCACAGCAC 
TACATCTCCG AGCCCTATCT GGCCCTCCAT GAAGACTCCC ACGCCTCAGA CTGTGGCGAG 
GAGGAGACAC TCACCTTTGA GGCAGCCGTA GCCACTAGCT TGGGCCGGTC CAATACCATT 
GGCTCTGCGC CACCCCTACG GCACAGCTGG CAGATGCCTA ATGGGCACTA TCGGCGGCGG 
AGGCGTGGGG GGCCTGGGCC TGGCATGATG TGTGGAGCTG TCAGTGACCT CCTGAGTGAC 
ACGGAAGAAG ACGATAAGTG CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cacna1e ( XM_017312712.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017168201.1 Mus musculus calcium channel, voltage-dependent, R type, alpha 1E subunit (Cacna1e), transcript variant X11, mRNA.

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CloneID	OMu67265
Clone ID Related Accession (Same CDS sequence)	XM_017312712.1
Accession Version	XM_017312712.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7311bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	voltage-dependent R-type calcium channel subunit alpha-1E isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGCCCG AGATGCGTGG ACTGAGCCCA GGCCGAAGGC TGCCCGAGCC CACCCTGTGC 
CCGGCTACAG GAGGGAGCCC TGAGCCCGGG GCTGCTGGCA GGCGCCGCCA CGTCCTGGCT 
CTGCCTGATG AGGAGGCATG GCGGCCGCAG CAGTGCGCCC TGCTCGAGGC CAATGCCTCC 
GACGAGGTGG GCATGTTGCC TGCTTCCCCG CGCTCCGCGG TGGCCGGCTT CGACAACTTC 
TTCCCGGTGC AGGAAGGCGA GGGCCCGGGC TGGGAGGGCA GCACCACGCT GGATGCAGGC 
TCCAGCCCCT TCCTTCCTGT GAGCCCAGAG GTCATGAAGC GGAGGCGAGG GGGGCTCATC 
GAGCAGCGGG ACATCATCAA GGCCCACGAG GCACACAAGA TGCAGAGCAC CCCGCAGGCC 
CGGCGCAAGG AGTGGGAGAT GGCTCGCTTC GGGGAGGCGG TGGTCGTTGG CAGGCCAGGC 
TCAGGCGATG GAGACTCGGA CCAGAGCAGG AACCGACAAG GAACCCCCGT CCCGGCCTCG 
GGGCCGGCGG CCGCCTACAA GCAGTCAAAA GCGCAGAGGG CGCGGACTAT GGCTTTGTAC 
AACCCCATAC CAGTCCGGCA GAACTGTTTC ACGGTCAACA GATCCCTGTT CATCTTCGGA 
GAAGATAACA TTGTCAGGAA ATACGCCAAG AAGCTCATCG ATTGGCCGCC ATTTGAGTAC 
ATGATCCTGG CCACCATCAT TGCCAACTGC ATCGTCTTGG CCCTGGAGCA GCATCTTCCT 
GAGGATGACA AGACCCCAAT GTCTCGAAGA CTGGAGAAGA CAGAACCATA TTTCATTGGG 
ATCTTCTGCT TTGAAGCTGG GATCAAAATT GTGGCTCTAG GGTTCATCTT CCATAAGGGT 
TCATACCTCC GTAATGGCTG GAATGTCATG GACTTCATCG TGGTCCTTAG TGGCATCCTG 
GCCACTGCGG GAACCCACTT CAACACCCAC GTGGACCTGC GGACCCTCCG GGCTGTGCGT 
GTCCTGAGGC CTTTAAAGCT GGTATCAGGA ATACCTAGCC TGCAGATCGT GCTGAAGTCT 
ATCATGAAGG CCATGGTGCC TCTTCTGCAG ATTGGCCTTC TGCTCTTCTT TGCCATCCTC 
ATGTTTGCTA TCATTGGCTT GGAGTTCTAC AGTGGCAAGT TGCATCGAGC ATGCTTCATG 
AACAATTCAG GTATTCTAGA GGGGTTTGAT CCTCCTCACC CGTGTGGTGT GCAAGGCTGC 
CCGGCTGGTT ATGAATGTAA GGACTGGATC GGCCCCAATG ACGGGATCAC CCAGTTTGAC 
AACATCCTTT TTGCCGTGCT GACTGTCTTC CAATGCATCA CTATGGAAGG GTGGACCACT 
GTGCTGTACA ATACCAATGA TGCCTTAGGA GCCACCTGGA ACTGGCTGTA CTTCATCCCT 
CTCATCATCA TCGGATCCTT CTTTGTTCTC AACCTCGTCC TGGGAGTGCT TTCCGGGGAA 
TTTGCCAAAG AAAGAGAGAG AGTGGAGAAC CGAAGAGCGT TCATGAAGCT CCGGCGCCAG 
CAACAGATTG AGCGAGAATT GAACGGCTAC CGTGCCTGGA TAGACAAAGC AGAGGAAGTC 
ATGCTTGCTG AAGAAAATAA AAACTCGGGA ACATCAGCCT TGGAAGGCAC ACCTCTTGCC 
AGAGCCAGTA TCAAGAGCAC AAAAGTAGAT GGGGCCTCCT ATTTCCGGCA CAAGGAACGG 
CTTCTGCGCA TCTCTATCCG CCACATGGTC AAGTCCCAAG TCTTTTACTG GATCGTCCTG 
AGTGTTGTGG CTCTTAATAC TGCCTGTGTG GCCATTGTTC ATCACAACCA GCCACAGTGG 
CTCACTCACC TCCTCTACTA TGCAGAGTTT TTGTTTCTGG GACTCTTCCT GTTGGAGATG 
TCCCTGAAGA TGTATGGCAT GGGGCCACGC CTCTATTTTC ACTCATCCTT CAACTGCTTT 
GATTTTGGGG TCACAGTGGG CAGCATCTTT GAGGTTGTCT GGGCAATCTT CAGACCTGGC 
ACATCTTTTG GAATCAGTGT CTTACGAGCT CTCCGGCTCC TAAGGATATT TAAAATAACC 
AAGTATTGGG CATCCCTCCG GAATCTGGTT GTCTCCTTGA TGAGCTCCAT GAAGTCTATC 
ATCAGCTTAC TCTTCCTTCT CTTCCTCTTC ATCGTTGTCT TTGCTCTCCT GGGAATGCAG 
CTCTTTGGAG GCAGGTTTAA CTTTAATGAT GGGACTCCTT CAGCTAATTT TGACACCTTC 
CCTGCAGCTA TCATGACAGT GTTCCAGATC CTGACAGGGG AAGACTGGAA TGAGGTGATG 
TACAATGGCA TTCGCTCCCA GGGTGGGGTC AGCTCAGGCA TGTGGTCTGC CATCTACTTC 
ATTGTGCTCA CCTTGTTTGG AAACTACACG CTGCTAAATG TATTCTTGGC CATCGCTGTG 
GACAATCTGG CCAATGCCCA GGAACTGACC AAGGATGAGC AGGAGGAAGA AGAAGCCTTC 
AACCAGAAAC ATGCACTGCA GAAGGCCAAG GAGGTCAGCC CGATGTCTGC TCCCAACATG 
CCTTCCATTG AAAGAGACAG AAGGAGAAGA CACCACATGT CGATGTGGGA GCCACGCAGC 
AGCCACCTGA GGGAGCGGAG GCGCCGGCAC CACATGTCTG TGTGGGAACA ACGCACCAGC 
CAGCTGAGGA GGCACATGCA AATGTCTAGC CAAGAAGCCC TCAACAAAGA GGAGGCCCCG 
CCAATGAACC CCCTCAATCC ACTCAACCCA CTGAGTCCTC TCAACCCCCT CAATGCTCAC 
CCCAGCCTTT ATCGGAGGCC CAGGCCCATT GAGGGCCTAG CCCTAGGTCT GGGTCTTGAA 
AAGTGTGAAG AAGAACGCAT CAGTCGTGGG GGCTCCCTCA AGGGGGACAT AGGGGGCCTG 
ACTAGCGCCC TGGACAACCA GAGGAGCCCC TTGTCCTTGG GCAAACGGGA GCCACCGTGG 
CTGCCTAGAT CCTGTCATGG AAACTGTGAC CCAATCCAGC AGGAGGCTGG GGGAGGAGAG 
ACTGTGGTGA CTTTTGAGGA CCGGGCCAGG CACAGGCAGA GTCAGAGGCG AAGCCGGCAT 
CGCCGAGTCA GGACTGAGGG CAAGGACTCT GCCTCTGCAT CCCGAAGCAG GTCTGCCAGC 
CAGGAGCGGA GTCTGGATGA AGGGGTGTCC GTTGAGGGGG AGAAGGAACA TGAGCCTCAC 
AGCAGCCACA GGAGCAAAGA GCCGACCATC CACGAAGAGG AGAGAACCCA GGACTTAAGG 
AGGACCAACA GTCTGATGGT CCCCAGGGGC TCTGGACTGG TAGGGGCTCT TGATGAAGCA 
GAAACACCTC TAGTCCAGCC CCAACCTGAG CTGGAAGTGG GGAAGGATGC AGCTCTGACA 
GAGCAGGAGG CTGAAGGCAG CAGTGAACAA GCCCTTCTTG GGGACGTACA GCTGGATGTG 
GGCCGGGGCA TTAGCCAGAG TGAACCGGAT CTCTCCTGCA TGACAGCCAA CATGGACAAG 
GCTACCACGG AGAGCACCAG TGTCACGGTC GCCATCCCTG ATGTGGACCC CTTGGTGGAC 
TCGACAGTGG TGAACATTAG CAACAAGACG GATGGAGAAG CCAGTCCCTT GAAGGAGGCA 
GAGACTAAAG AGGAAGAAGA AGAGGTAGAG AAGAAGAAGA AGCAGAAGAA GGAGAAGCGA 
GAGACTGGAA AAGCCATGGT GCCCCATAGC TCTATGTTCA TCTTCAGCAC CACTAACCCG 
ATCCGCAGGG CCTGCCACTA CATTGTGAAC CTACGCTACT TTGAAATGTG CATTCTCTTG 
GTGATTGCGG CCAGCAGCAT TGCCCTGGCA GCCGAGGACC CTGTACTAAC TAACTCGGAG 
CGTAACAAAG TCCTGAGATA TTTCGACTAT GTTTTCACAG GAGTGTTTAC CTTTGAGATG 
GTTATAAAGA TGATAGACCA AGGCTTGATC CTGCAGGATG GATCATACTT CAGAGACCTC 
TGGAACATCC TGGACTTTGT AGTAGTGGTT GGTGCATTGG TAGCATTTGC TCTGGCGAAC 
GCTTTGGGAA CCAACAAGGG GAGGGATATC AAGACTATCA AGTCTCTGAG GGTACTTCGA 
GTTCTGCGGC CACTGAAAAC CATCAAGCGC TTGCCCAAGC TGAAGGCTGT GTTTGACTGC 
GTGGTGACCT CCTTGAAGAA TGTCTTCAAC ATACTCATTG TCTACAAGCT CTTCATGTTC 
ATCTTTGCCG TCATCGCTGT TCAGCTATTC AAAGGAAAGT TCTTTTATTG CACTGACAGT 
TCCAAGGACA CAGAGAAGGA GTGCATAGGC AACTATGTAG ACCACGAGAA AAACAAGATG 
GAGGTGAAGG GCCGGGAATG GAAGCGTCAT GAATTCCACT ATGACAACAT CATCTGGGCC 
CTGCTTACCC TCTTCACAGT CTCTACAGGG GAAGGATGGC CTCAGGTTCT GCAGCACTCT 
GTAGATGTGA CAGAGGAGGA CAGAGGCCCA AGTCGCAGCA ACCGCATGGA GATGTCCATC 
TTTTATGTGG TCTACTTTGT GGTCTTCCCT TTCTTCTTTG TCAATATCTT CGTGGCTCTC 
ATTATCATCA CCTTCCAGGA GCAAGGGGAC AAGATGATGG AGGAGTGTAG CCTGGAGAAG 
AATGAGCGGG CATGCATCGA CTTCGCCATT AGTGCCAAAC CTCTCACCCG CTACATGCCA 
CAGAACAGGC ACACCTTCCA GTACCGTGTG TGGCACTTTG TAGTCTCCCC ATCCTTTGAG 
TATACCATCA TGGCCATGAT TGCCTTGAAC ACTGTCGTGC TGATGATGAA GTACTACTCT 
GCTCCTTGCA CCTATGAGCT CGCCTTAAAG TACCTGAACA TCGCCTTCAC TATGGTGTTT 
TCCCTGGAGT GTGTCCTAAA GGTCATTGCT TTCGGCTTCC TGAACTATTT CCGAGATACC 
TGGAACATCT TTGACTTCAT CACGGTGATC GGCAGTATCA CAGAAATTAT CCTGACAGAT 
AGCAAGCTGG TGAACACCAG TGGCTTCAAT ATGAGCTTTC TGAAGCTCTT CCGAGCTGCA 
CGGCTCATAA AGCTTCTGAG ACAGGGCTAC ACCATCCGCA TCTTACTGTG GACCTTCGTG 
CAGTCCTTTA AGGCTCTTCC CTACGTCTGC CTTCTCATTG CCATGCTTTT CTTCATCTAC 
GCCATCATTG GGATGCAGGT GTTTGGAAAC ATAAAGTTAG ATGAGGAGAG TCACATCAAC 
CGGCACAACA ACTTCCGGAG TTTCTTTGGA TCCCTCATGC TGCTTTTCAG GAGTGCCACG 
GGGGAGGCTT GGCAGGAGAT TATGCTGTCC TGTCTCGGTG AGAAGGGTTG TGAGCCTGAC 
ACCACTGCAC CCTCAGGGCA GAATGAGAGT GAGCGCTGTG GCACTGACCT GGCCTACGTT 
TATTTTGTCT CCTTCATCTT CTTCTGCTCC TTCCTGATGC TCAACCTGTT CGTGGCTGTC 
ATCATGGACA ACTTTGAGTA CCTGACTCGA GATTCCTCCA TCCTGGGGCC TCACCACTTA 
GATGAGTTTG TCCGTGTCTG GGCAGAATAT GACAGAGCAG CATGTGGCCG CATCCATTAC 
ACTGAGATGT ATGAAATGCT GACTCTCATG TCACCACCGC TAGGCCTCGG CAAGAGATGT 
CCCTCCAAAG TGGCCTATAA GAGGCTGGTC CTGATGAACA TGCCAGTGGC TGAGGACATG 
ACAGTCCACT TCACCTCCAC ACTTATGGCT CTGATCCGGA CAGCTCTGGA CATTAAAATC 
GCCAAAGGTG GTGCAGACAG ACAGCAGCTA GACTCGGAGC TGCAAAAAGA GACCCTGGCC 
ATTTGGCCTC ACCTGTCCCA AAAGATGTTG GATCTGCTTG TGCCCATGCC CAAAGCCTCT 
GACCTGACTG TGGGCAAGAT CTATGCAGCA ATGATGATCA TGGACTACTA TAAACAGAGT 
AAGGTAAAGA AACAGAGACA GCAGCTGGAA GAACAGAAAA ATGCACCCAT GTTCCAGCGC 
ATGGAGCCCT CATCACTGCC TCAGGAGATC ATTGCTAATG CCAAAGCCCT GCCTTATCTC 
CAGCAGGACC CTGTTTCTGG CCTGAGTGGT CGGAGTGGAT ACCCTTCAAT GAGTCCACTC 
TCCCCTCAAG AAATATTCCA GTTGGCTTGT ATGGACCCAG CGGATGATGG ACAATTCCAG 
GAACAACAAT CTCTGGTGAA TGCACAAGAG CCAGAGGTTA GTGAATTAAA AAGTGTGCAG 
TCCTCTAACC ATGGCATCTA CCTTCCTCCG GACACCCAGG AGCATGCGGG ATCTGGGAGG 
GCATCCTCTA TGCCACGTCT GACTATGGAT CCCCAGGTGG TAACAGACCC TAGCTCTATG 
CGACGTTCAT TTTCTACAAT TCGGGATAAG CGTTCAAATT CCTCCTGGTT GGAGGAGTTC 
TCCATGGAAC GCAGCAGTGA AAACACTTAC AAGTCGCGGC GCAGAAGTTA CCATTCCTCC 
CTGAGGCTGT CAGCACACCG CCTGAATTCT GACTCTGGTC ACAAGTCTGA CACTCACCGA 
TCAGGAGGCA GGGAGAGAGG CCGATCCAAA GAACGAAAGC ACCTCCTCTC TCCAGATGTC 
TCCCGCTGCA ATTCTGAAGA GCGAGGGACC CAGGCCGACT GGGAGTCCCC AGAACGTCGC 
CAGTCCAGGT CACCCAGTGA AGGAAGGTCA CAGACCCCCA ATAGACAGGG TACCGGTTCC 
CTAAGTGAGA GCTCCATCCC CTCTATTTCC GACACCAGCA CCCCAAGACG AAGCCGTCGC 
CAGCTCCCAC CTGTGCCACC AAAGCCTCGT CCCCTCCTCT CCTACAGCTC CCTGATGAGA 
CACACTGGCG GCATCTCTCC ACCTCCTGAT GGAAGTGAGG GTGGATCCCC ACTGGCCTCT 
CAAGCTCTGG AGAGCAACAG CGCTTGCCTG ACCGAGTCTT CCAACTCCTT GCACCCCCAG 
CAGGGCCAGC ATCCTTCCCC ACAGCACTAC ATCTCCGAGC CCTATCTGGC CCTCCATGAA 
GACTCCCACG CCTCAGACTG TGGCGAGGAG GAGACACTCA CCTTTGAGGC AGCCGTAGCC 
ACTAGCTTGG GCCGGTCCAA TACCATTGGC TCTGCGCCAC CCCTACGGCA CAGCTGGCAG 
ATGCCTAATG GGCACTATCG GCGGCGGAGG CGTGGGGGGC CTGGGCCTGG CATGATGTGT 
GGAGCTGTCA GTGACCTCCT GAGTGACACG GAAGAAGACG ATAAGTGCTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017168201.1
CDS	594..7904
Translation	MVPEMRGLSPGRRLPEPTLCPATGGSPEPGAAGRRRHVLALPDEEAWRPQQCALLEANASDEVGMLPASPRSAVAGFDNFFPVQEGEGPGWEGSTTLDAGSSPFLPVSPEVMKRRRGGLIEQRDIIKAHEAHKMQSTPQARRKEWEMARFGEAVVVGRPGSGDGDSDQSRNRQGTPVPASGPAAAYKQSKAQRARTMALYNPIPVRQNCFTVNRSLFIFGEDNIVRKYAKKLIDWPPFEYMILATIIANCIVLALEQHLPEDDKTPMSRRLEKTEPYFIGIFCFEAGIKIVALGFIFHKGSYLRNGWNVMDFIVVLSGILATAGTHFNTHVDLRTLRAVRVLRPLKLVSGIPSLQIVLKSIMKAMVPLLQIGLLLFFAILMFAIIGLEFYSGKLHRACFMNNSGILEGFDPPHPCGVQGCPAGYECKDWIGPNDGITQFDNILFAVLTVFQCITMEGWTTVLYNTNDALGATWNWLYFIPLIIIGSFFVLNLVLGVLSGEFAKERERVENRRAFMKLRRQQQIERELNGYRAWIDKAEEVMLAEENKNSGTSALEGTPLARASIKSTKVDGASYFRHKERLLRISIRHMVKSQVFYWIVLSVVALNTACVAIVHHNQPQWLTHLLYYAEFLFLGLFLLEMSLKMYGMGPRLYFHSSFNCFDFGVTVGSIFEVVWAIFRPGTSFGISVLRALRLLRIFKITKYWASLRNLVVSLMSSMKSIISLLFLLFLFIVVFALLGMQLFGGRFNFNDGTPSANFDTFPAAIMTVFQILTGEDWNEVMYNGIRSQGGVSSGMWSAIYFIVLTLFGNYTLLNVFLAIAVDNLANAQELTKDEQEEEEAFNQKHALQKAKEVSPMSAPNMPSIERDRRRRHHMSMWEPRSSHLRERRRRHHMSVWEQRTSQLRRHMQMSSQEALNKEEAPPMNPLNPLNPLSPLNPLNAHPSLYRRPRPIEGLALGLGLEKCEEERISRGGSLKGDIGGLTSALDNQRSPLSLGKREPPWLPRSCHGNCDPIQQEAGGGETVVTFEDRARHRQSQRRSRHRRVRTEGKDSASASRSRSASQERSLDEGVSVEGEKEHEPHSSHRSKEPTIHEEERTQDLRRTNSLMVPRGSGLVGALDEAETPLVQPQPELEVGKDAALTEQEAEGSSEQALLGDVQLDVGRGISQSEPDLSCMTANMDKATTESTSVTVAIPDVDPLVDSTVVNISNKTDGEASPLKEAETKEEEEEVEKKKKQKKEKRETGKAMVPHSSMFIFSTTNPIRRACHYIVNLRYFEMCILLVIAASSIALAAEDPVLTNSERNKVLRYFDYVFTGVFTFEMVIKMIDQGLILQDGSYFRDLWNILDFVVVVGALVAFALANALGTNKGRDIKTIKSLRVLRVLRPLKTIKRLPKLKAVFDCVVTSLKNVFNILIVYKLFMFIFAVIAVQLFKGKFFYCTDSSKDTEKECIGNYVDHEKNKMEVKGREWKRHEFHYDNIIWALLTLFTVSTGEGWPQVLQHSVDVTEEDRGPSRSNRMEMSIFYVVYFVVFPFFFVNIFVALIIITFQEQGDKMMEECSLEKNERACIDFAISAKPLTRYMPQNRHTFQYRVWHFVVSPSFEYTIMAMIALNTVVLMMKYYSAPCTYELALKYLNIAFTMVFSLECVLKVIAFGFLNYFRDTWNIFDFITVIGSITEIILTDSKLVNTSGFNMSFLKLFRAARLIKLLRQGYTIRILLWTFVQSFKALPYVCLLIAMLFFIYAIIGMQVFGNIKLDEESHINRHNNFRSFFGSLMLLFRSATGEAWQEIMLSCLGEKGCEPDTTAPSGQNESERCGTDLAYVYFVSFIFFCSFLMLNLFVAVIMDNFEYLTRDSSILGPHHLDEFVRVWAEYDRAACGRIHYTEMYEMLTLMSPPLGLGKRCPSKVAYKRLVLMNMPVAEDMTVHFTSTLMALIRTALDIKIAKGGADRQQLDSELQKETLAIWPHLSQKMLDLLVPMPKASDLTVGKIYAAMMIMDYYKQSKVKKQRQQLEEQKNAPMFQRMEPSSLPQEIIANAKALPYLQQDPVSGLSGRSGYPSMSPLSPQEIFQLACMDPADDGQFQEQQSLVNAQEPEVSELKSVQSSNHGIYLPPDTQEHAGSGRASSMPRLTMDPQVVTDPSSMRRSFSTIRDKRSNSSWLEEFSMERSSENTYKSRRRSYHSSLRLSAHRLNSDSGHKSDTHRSGGRERGRSKERKHLLSPDVSRCNSEERGTQADWESPERRQSRSPSEGRSQTPNRQGTGSLSESSIPSISDTSTPRRSRRQLPPVPPKPRPLLSYSSLMRHTGGISPPPDGSEGGSPLASQALESNSACLTESSNSLHPQQGQHPSPQHYISEPYLALHEDSHASDCGEEETLTFEAAVATSLGRSNTIGSAPPLRHSWQMPNGHYRRRRRGGPGPGMMCGAVSDLLSDTEEDDKC

Target ORF information:

RefSeq Version	XM_017312712.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus calcium channel, voltage-dependent, R type, alpha 1E subunit (Cacna1e), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017312712.1

ORF Insert Sequence:

ATGGTGCCCG AGATGCGTGG ACTGAGCCCA GGCCGAAGGC TGCCCGAGCC CACCCTGTGC 
CCGGCTACAG GAGGGAGCCC TGAGCCCGGG GCTGCTGGCA GGCGCCGCCA CGTCCTGGCT 
CTGCCTGATG AGGAGGCATG GCGGCCGCAG CAGTGCGCCC TGCTCGAGGC CAATGCCTCC 
GACGAGGTGG GCATGTTGCC TGCTTCCCCG CGCTCCGCGG TGGCCGGCTT CGACAACTTC 
TTCCCGGTGC AGGAAGGCGA GGGCCCGGGC TGGGAGGGCA GCACCACGCT GGATGCAGGC 
TCCAGCCCCT TCCTTCCTGT GAGCCCAGAG GTCATGAAGC GGAGGCGAGG GGGGCTCATC 
GAGCAGCGGG ACATCATCAA GGCCCACGAG GCACACAAGA TGCAGAGCAC CCCGCAGGCC 
CGGCGCAAGG AGTGGGAGAT GGCTCGCTTC GGGGAGGCGG TGGTCGTTGG CAGGCCAGGC 
TCAGGCGATG GAGACTCGGA CCAGAGCAGG AACCGACAAG GAACCCCCGT CCCGGCCTCG 
GGGCCGGCGG CCGCCTACAA GCAGTCAAAA GCGCAGAGGG CGCGGACTAT GGCTTTGTAC 
AACCCCATAC CAGTCCGGCA GAACTGTTTC ACGGTCAACA GATCCCTGTT CATCTTCGGA 
GAAGATAACA TTGTCAGGAA ATACGCCAAG AAGCTCATCG ATTGGCCGCC ATTTGAGTAC 
ATGATCCTGG CCACCATCAT TGCCAACTGC ATCGTCTTGG CCCTGGAGCA GCATCTTCCT 
GAGGATGACA AGACCCCAAT GTCTCGAAGA CTGGAGAAGA CAGAACCATA TTTCATTGGG 
ATCTTCTGCT TTGAAGCTGG GATCAAAATT GTGGCTCTAG GGTTCATCTT CCATAAGGGT 
TCATACCTCC GTAATGGCTG GAATGTCATG GACTTCATCG TGGTCCTTAG TGGCATCCTG 
GCCACTGCGG GAACCCACTT CAACACCCAC GTGGACCTGC GGACCCTCCG GGCTGTGCGT 
GTCCTGAGGC CTTTAAAGCT GGTATCAGGA ATACCTAGCC TGCAGATCGT GCTGAAGTCT 
ATCATGAAGG CCATGGTGCC TCTTCTGCAG ATTGGCCTTC TGCTCTTCTT TGCCATCCTC 
ATGTTTGCTA TCATTGGCTT GGAGTTCTAC AGTGGCAAGT TGCATCGAGC ATGCTTCATG 
AACAATTCAG GTATTCTAGA GGGGTTTGAT CCTCCTCACC CGTGTGGTGT GCAAGGCTGC 
CCGGCTGGTT ATGAATGTAA GGACTGGATC GGCCCCAATG ACGGGATCAC CCAGTTTGAC 
AACATCCTTT TTGCCGTGCT GACTGTCTTC CAATGCATCA CTATGGAAGG GTGGACCACT 
GTGCTGTACA ATACCAATGA TGCCTTAGGA GCCACCTGGA ACTGGCTGTA CTTCATCCCT 
CTCATCATCA TCGGATCCTT CTTTGTTCTC AACCTCGTCC TGGGAGTGCT TTCCGGGGAA 
TTTGCCAAAG AAAGAGAGAG AGTGGAGAAC CGAAGAGCGT TCATGAAGCT CCGGCGCCAG 
CAACAGATTG AGCGAGAATT GAACGGCTAC CGTGCCTGGA TAGACAAAGC AGAGGAAGTC 
ATGCTTGCTG AAGAAAATAA AAACTCGGGA ACATCAGCCT TGGAAGGCAC ACCTCTTGCC 
AGAGCCAGTA TCAAGAGCAC AAAAGTAGAT GGGGCCTCCT ATTTCCGGCA CAAGGAACGG 
CTTCTGCGCA TCTCTATCCG CCACATGGTC AAGTCCCAAG TCTTTTACTG GATCGTCCTG 
AGTGTTGTGG CTCTTAATAC TGCCTGTGTG GCCATTGTTC ATCACAACCA GCCACAGTGG 
CTCACTCACC TCCTCTACTA TGCAGAGTTT TTGTTTCTGG GACTCTTCCT GTTGGAGATG 
TCCCTGAAGA TGTATGGCAT GGGGCCACGC CTCTATTTTC ACTCATCCTT CAACTGCTTT 
GATTTTGGGG TCACAGTGGG CAGCATCTTT GAGGTTGTCT GGGCAATCTT CAGACCTGGC 
ACATCTTTTG GAATCAGTGT CTTACGAGCT CTCCGGCTCC TAAGGATATT TAAAATAACC 
AAGTATTGGG CATCCCTCCG GAATCTGGTT GTCTCCTTGA TGAGCTCCAT GAAGTCTATC 
ATCAGCTTAC TCTTCCTTCT CTTCCTCTTC ATCGTTGTCT TTGCTCTCCT GGGAATGCAG 
CTCTTTGGAG GCAGGTTTAA CTTTAATGAT GGGACTCCTT CAGCTAATTT TGACACCTTC 
CCTGCAGCTA TCATGACAGT GTTCCAGATC CTGACAGGGG AAGACTGGAA TGAGGTGATG 
TACAATGGCA TTCGCTCCCA GGGTGGGGTC AGCTCAGGCA TGTGGTCTGC CATCTACTTC 
ATTGTGCTCA CCTTGTTTGG AAACTACACG CTGCTAAATG TATTCTTGGC CATCGCTGTG 
GACAATCTGG CCAATGCCCA GGAACTGACC AAGGATGAGC AGGAGGAAGA AGAAGCCTTC 
AACCAGAAAC ATGCACTGCA GAAGGCCAAG GAGGTCAGCC CGATGTCTGC TCCCAACATG 
CCTTCCATTG AAAGAGACAG AAGGAGAAGA CACCACATGT CGATGTGGGA GCCACGCAGC 
AGCCACCTGA GGGAGCGGAG GCGCCGGCAC CACATGTCTG TGTGGGAACA ACGCACCAGC 
CAGCTGAGGA GGCACATGCA AATGTCTAGC CAAGAAGCCC TCAACAAAGA GGAGGCCCCG 
CCAATGAACC CCCTCAATCC ACTCAACCCA CTGAGTCCTC TCAACCCCCT CAATGCTCAC 
CCCAGCCTTT ATCGGAGGCC CAGGCCCATT GAGGGCCTAG CCCTAGGTCT GGGTCTTGAA 
AAGTGTGAAG AAGAACGCAT CAGTCGTGGG GGCTCCCTCA AGGGGGACAT AGGGGGCCTG 
ACTAGCGCCC TGGACAACCA GAGGAGCCCC TTGTCCTTGG GCAAACGGGA GCCACCGTGG 
CTGCCTAGAT CCTGTCATGG AAACTGTGAC CCAATCCAGC AGGAGGCTGG GGGAGGAGAG 
ACTGTGGTGA CTTTTGAGGA CCGGGCCAGG CACAGGCAGA GTCAGAGGCG AAGCCGGCAT 
CGCCGAGTCA GGACTGAGGG CAAGGACTCT GCCTCTGCAT CCCGAAGCAG GTCTGCCAGC 
CAGGAGCGGA GTCTGGATGA AGGGGTGTCC GTTGAGGGGG AGAAGGAACA TGAGCCTCAC 
AGCAGCCACA GGAGCAAAGA GCCGACCATC CACGAAGAGG AGAGAACCCA GGACTTAAGG 
AGGACCAACA GTCTGATGGT CCCCAGGGGC TCTGGACTGG TAGGGGCTCT TGATGAAGCA 
GAAACACCTC TAGTCCAGCC CCAACCTGAG CTGGAAGTGG GGAAGGATGC AGCTCTGACA 
GAGCAGGAGG CTGAAGGCAG CAGTGAACAA GCCCTTCTTG GGGACGTACA GCTGGATGTG 
GGCCGGGGCA TTAGCCAGAG TGAACCGGAT CTCTCCTGCA TGACAGCCAA CATGGACAAG 
GCTACCACGG AGAGCACCAG TGTCACGGTC GCCATCCCTG ATGTGGACCC CTTGGTGGAC 
TCGACAGTGG TGAACATTAG CAACAAGACG GATGGAGAAG CCAGTCCCTT GAAGGAGGCA 
GAGACTAAAG AGGAAGAAGA AGAGGTAGAG AAGAAGAAGA AGCAGAAGAA GGAGAAGCGA 
GAGACTGGAA AAGCCATGGT GCCCCATAGC TCTATGTTCA TCTTCAGCAC CACTAACCCG 
ATCCGCAGGG CCTGCCACTA CATTGTGAAC CTACGCTACT TTGAAATGTG CATTCTCTTG 
GTGATTGCGG CCAGCAGCAT TGCCCTGGCA GCCGAGGACC CTGTACTAAC TAACTCGGAG 
CGTAACAAAG TCCTGAGATA TTTCGACTAT GTTTTCACAG GAGTGTTTAC CTTTGAGATG 
GTTATAAAGA TGATAGACCA AGGCTTGATC CTGCAGGATG GATCATACTT CAGAGACCTC 
TGGAACATCC TGGACTTTGT AGTAGTGGTT GGTGCATTGG TAGCATTTGC TCTGGCGAAC 
GCTTTGGGAA CCAACAAGGG GAGGGATATC AAGACTATCA AGTCTCTGAG GGTACTTCGA 
GTTCTGCGGC CACTGAAAAC CATCAAGCGC TTGCCCAAGC TGAAGGCTGT GTTTGACTGC 
GTGGTGACCT CCTTGAAGAA TGTCTTCAAC ATACTCATTG TCTACAAGCT CTTCATGTTC 
ATCTTTGCCG TCATCGCTGT TCAGCTATTC AAAGGAAAGT TCTTTTATTG CACTGACAGT 
TCCAAGGACA CAGAGAAGGA GTGCATAGGC AACTATGTAG ACCACGAGAA AAACAAGATG 
GAGGTGAAGG GCCGGGAATG GAAGCGTCAT GAATTCCACT ATGACAACAT CATCTGGGCC 
CTGCTTACCC TCTTCACAGT CTCTACAGGG GAAGGATGGC CTCAGGTTCT GCAGCACTCT 
GTAGATGTGA CAGAGGAGGA CAGAGGCCCA AGTCGCAGCA ACCGCATGGA GATGTCCATC 
TTTTATGTGG TCTACTTTGT GGTCTTCCCT TTCTTCTTTG TCAATATCTT CGTGGCTCTC 
ATTATCATCA CCTTCCAGGA GCAAGGGGAC AAGATGATGG AGGAGTGTAG CCTGGAGAAG 
AATGAGCGGG CATGCATCGA CTTCGCCATT AGTGCCAAAC CTCTCACCCG CTACATGCCA 
CAGAACAGGC ACACCTTCCA GTACCGTGTG TGGCACTTTG TAGTCTCCCC ATCCTTTGAG 
TATACCATCA TGGCCATGAT TGCCTTGAAC ACTGTCGTGC TGATGATGAA GTACTACTCT 
GCTCCTTGCA CCTATGAGCT CGCCTTAAAG TACCTGAACA TCGCCTTCAC TATGGTGTTT 
TCCCTGGAGT GTGTCCTAAA GGTCATTGCT TTCGGCTTCC TGAACTATTT CCGAGATACC 
TGGAACATCT TTGACTTCAT CACGGTGATC GGCAGTATCA CAGAAATTAT CCTGACAGAT 
AGCAAGCTGG TGAACACCAG TGGCTTCAAT ATGAGCTTTC TGAAGCTCTT CCGAGCTGCA 
CGGCTCATAA AGCTTCTGAG ACAGGGCTAC ACCATCCGCA TCTTACTGTG GACCTTCGTG 
CAGTCCTTTA AGGCTCTTCC CTACGTCTGC CTTCTCATTG CCATGCTTTT CTTCATCTAC 
GCCATCATTG GGATGCAGGT GTTTGGAAAC ATAAAGTTAG ATGAGGAGAG TCACATCAAC 
CGGCACAACA ACTTCCGGAG TTTCTTTGGA TCCCTCATGC TGCTTTTCAG GAGTGCCACG 
GGGGAGGCTT GGCAGGAGAT TATGCTGTCC TGTCTCGGTG AGAAGGGTTG TGAGCCTGAC 
ACCACTGCAC CCTCAGGGCA GAATGAGAGT GAGCGCTGTG GCACTGACCT GGCCTACGTT 
TATTTTGTCT CCTTCATCTT CTTCTGCTCC TTCCTGATGC TCAACCTGTT CGTGGCTGTC 
ATCATGGACA ACTTTGAGTA CCTGACTCGA GATTCCTCCA TCCTGGGGCC TCACCACTTA 
GATGAGTTTG TCCGTGTCTG GGCAGAATAT GACAGAGCAG CATGTGGCCG CATCCATTAC 
ACTGAGATGT ATGAAATGCT GACTCTCATG TCACCACCGC TAGGCCTCGG CAAGAGATGT 
CCCTCCAAAG TGGCCTATAA GAGGCTGGTC CTGATGAACA TGCCAGTGGC TGAGGACATG 
ACAGTCCACT TCACCTCCAC ACTTATGGCT CTGATCCGGA CAGCTCTGGA CATTAAAATC 
GCCAAAGGTG GTGCAGACAG ACAGCAGCTA GACTCGGAGC TGCAAAAAGA GACCCTGGCC 
ATTTGGCCTC ACCTGTCCCA AAAGATGTTG GATCTGCTTG TGCCCATGCC CAAAGCCTCT 
GACCTGACTG TGGGCAAGAT CTATGCAGCA ATGATGATCA TGGACTACTA TAAACAGAGT 
AAGGTAAAGA AACAGAGACA GCAGCTGGAA GAACAGAAAA ATGCACCCAT GTTCCAGCGC 
ATGGAGCCCT CATCACTGCC TCAGGAGATC ATTGCTAATG CCAAAGCCCT GCCTTATCTC 
CAGCAGGACC CTGTTTCTGG CCTGAGTGGT CGGAGTGGAT ACCCTTCAAT GAGTCCACTC 
TCCCCTCAAG AAATATTCCA GTTGGCTTGT ATGGACCCAG CGGATGATGG ACAATTCCAG 
GAACAACAAT CTCTGGTGAA TGCACAAGAG CCAGAGGTTA GTGAATTAAA AAGTGTGCAG 
TCCTCTAACC ATGGCATCTA CCTTCCTCCG GACACCCAGG AGCATGCGGG ATCTGGGAGG 
GCATCCTCTA TGCCACGTCT GACTATGGAT CCCCAGGTGG TAACAGACCC TAGCTCTATG 
CGACGTTCAT TTTCTACAAT TCGGGATAAG CGTTCAAATT CCTCCTGGTT GGAGGAGTTC 
TCCATGGAAC GCAGCAGTGA AAACACTTAC AAGTCGCGGC GCAGAAGTTA CCATTCCTCC 
CTGAGGCTGT CAGCACACCG CCTGAATTCT GACTCTGGTC ACAAGTCTGA CACTCACCGA 
TCAGGAGGCA GGGAGAGAGG CCGATCCAAA GAACGAAAGC ACCTCCTCTC TCCAGATGTC 
TCCCGCTGCA ATTCTGAAGA GCGAGGGACC CAGGCCGACT GGGAGTCCCC AGAACGTCGC 
CAGTCCAGGT CACCCAGTGA AGGAAGGTCA CAGACCCCCA ATAGACAGGG TACCGGTTCC 
CTAAGTGAGA GCTCCATCCC CTCTATTTCC GACACCAGCA CCCCAAGACG AAGCCGTCGC 
CAGCTCCCAC CTGTGCCACC AAAGCCTCGT CCCCTCCTCT CCTACAGCTC CCTGATGAGA 
CACACTGGCG GCATCTCTCC ACCTCCTGAT GGAAGTGAGG GTGGATCCCC ACTGGCCTCT 
CAAGCTCTGG AGAGCAACAG CGCTTGCCTG ACCGAGTCTT CCAACTCCTT GCACCCCCAG 
CAGGGCCAGC ATCCTTCCCC ACAGCACTAC ATCTCCGAGC CCTATCTGGC CCTCCATGAA 
GACTCCCACG CCTCAGACTG TGGCGAGGAG GAGACACTCA CCTTTGAGGC AGCCGTAGCC 
ACTAGCTTGG GCCGGTCCAA TACCATTGGC TCTGCGCCAC CCCTACGGCA CAGCTGGCAG 
ATGCCTAATG GGCACTATCG GCGGCGGAGG CGTGGGGGGC CTGGGCCTGG CATGATGTGT 
GGAGCTGTCA GTGACCTCCT GAGTGACACG GAAGAAGACG ATAAGTGCTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cacna1e ( XM_017312730.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017168219.1 Mus musculus calcium channel, voltage-dependent, R type, alpha 1E subunit (Cacna1e), transcript variant X12, mRNA.

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CloneID	OMu67266
Clone ID Related Accession (Same CDS sequence)	XM_017312730.1
Accession Version	XM_017312730.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7272bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	voltage-dependent R-type calcium channel subunit alpha-1E isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGCCCG AGATGCGTGG ACTGAGCCCA GGCCGAAGGC TGCCCGAGCC CACCCTGTGC 
CCGGCTACAG GAGGGAGCCC TGAGCCCGGG GCTGCTGGCA GGCGCCGCCA CGTCCTGGCT 
CTGCCTGATG AGGAGGCATG GCGGCCGCAG CAGTGCGCCC TGCTCGAGGC CAATGCCTCC 
GACGAGGTGG GCATGTTGCC TGCTTCCCCG CGCTCCGCGG TGGCCGGCTT CGACAACTTC 
TTCCCGGTGC AGGAAGGCGA GGGCCCGGGC TGGGAGGGCA GCACCACGCT GGATGCAGGC 
TCCAGCCCCT TCCTTCCTGT GAGCCCAGAG GTCATGAAGC GGAGGCGAGG GGGGCTCATC 
GAGCAGCGGG ACATCATCAA GGCCCACGAG GCACACAAGA TGCAGAGCAC CCCGCAGGCC 
CGGCGCAAGG AGTGGGAGAT GGCTCGCTTC GGGGAGGCGG TGGTCGTTGG CAGGCCAGGC 
TCAGGCGATG GAGACTCGGA CCAGAGCAGG AACCGACAAG GAACCCCCGT CCCGGCCTCG 
GGGCCGGCGG CCGCCTACAA GCAGTCAAAA GCGCAGAGGG CGCGGACTAT GGCTTTGTAC 
AACCCCATAC CAGTCCGGCA GAACTGTTTC ACGGTCAACA GATCCCTGTT CATCTTCGGA 
GAAGATAACA TTGTCAGGAA ATACGCCAAG AAGCTCATCG ATTGGCCGCC ATTTGAGTAC 
ATGATCCTGG CCACCATCAT TGCCAACTGC ATCGTCTTGG CCCTGGAGCA GCATCTTCCT 
GAGGATGACA AGACCCCAAT GTCTCGAAGA CTGGAGAAGA CAGAACCATA TTTCATTGGG 
ATCTTCTGCT TTGAAGCTGG GATCAAAATT GTGGCTCTAG GGTTCATCTT CCATAAGGGT 
TCATACCTCC GTAATGGCTG GAATGTCATG GACTTCATCG TGGTCCTTAG TGGCATCCTG 
GCCACTGCGG GAACCCACTT CAACACCCAC GTGGACCTGC GGACCCTCCG GGCTGTGCGT 
GTCCTGAGGC CTTTAAAGCT GGTATCAGGA ATACCTAGCC TGCAGATCGT GCTGAAGTCT 
ATCATGAAGG CCATGGTGCC TCTTCTGCAG ATTGGCCTTC TGCTCTTCTT TGCCATCCTC 
ATGTTTGCTA TCATTGGCTT GGAGTTCTAC AGTGGCAAGT TGCATCGAGC ATGCTTCATG 
AACAATTCAG GTATTCTAGA GGGGTTTGAT CCTCCTCACC CGTGTGGTGT GCAAGGCTGC 
CCGGCTGGTT ATGAATGTAA GGACTGGATC GGCCCCAATG ACGGGATCAC CCAGTTTGAC 
AACATCCTTT TTGCCGTGCT GACTGTCTTC CAATGCATCA CTATGGAAGG GTGGACCACT 
GTGCTGTACA ATACCAATGA TGCCTTAGGA GCCACCTGGA ACTGGCTGTA CTTCATCCCT 
CTCATCATCA TCGGATCCTT CTTTGTTCTC AACCTCGTCC TGGGAGTGCT TTCCGGGGAA 
TTTGCCAAAG AAAGAGAGAG AGTGGAGAAC CGAAGAGCGT TCATGAAGCT CCGGCGCCAG 
CAACAGATTG AGCGAGAATT GAACGGCTAC CGTGCCTGGA TAGACAAAGC AGAGGAAGTC 
ATGCTTGCTG AAGAAAATAA AAACTCGGGA ACATCAGCCT TGGAAGTGCT TCGAAGGGCA 
ACCATCAAAA GGAGCCGGAC GGAGGCCATG ACCCGAGACT CCAGCGATGA GCACTGCGTT 
GATATCTCTT CAGTGGGCAC ACCTCTTGCC AGAGCCAGTA TCAAGAGCAC AAAAGTAGAT 
GGGGCCTCCT ATTTCCGGCA CAAGGAACGG CTTCTGCGCA TCTCTATCCG CCACATGGTC 
AAGTCCCAAG TCTTTTACTG GATCGTCCTG AGTGTTGTGG CTCTTAATAC TGCCTGTGTG 
GCCATTGTTC ATCACAACCA GCCACAGTGG CTCACTCACC TCCTCTACTA TGCAGAGTTT 
TTGTTTCTGG GACTCTTCCT GTTGGAGATG TCCCTGAAGA TGTATGGCAT GGGGCCACGC 
CTCTATTTTC ACTCATCCTT CAACTGCTTT GATTTTGGGG TCACAGTGGG CAGCATCTTT 
GAGGTTGTCT GGGCAATCTT CAGACCTGGC ACATCTTTTG GAATCAGTGT CTTACGAGCT 
CTCCGGCTCC TAAGGATATT TAAAATAACC AAGTATTGGG CATCCCTCCG GAATCTGGTT 
GTCTCCTTGA TGAGCTCCAT GAAGTCTATC ATCAGCTTAC TCTTCCTTCT CTTCCTCTTC 
ATCGTTGTCT TTGCTCTCCT GGGAATGCAG CTCTTTGGAG GCAGGTTTAA CTTTAATGAT 
GGGACTCCTT CAGCTAATTT TGACACCTTC CCTGCAGCTA TCATGACAGT GTTCCAGATC 
CTGACAGGGG AAGACTGGAA TGAGGTGATG TACAATGGCA TTCGCTCCCA GGGTGGGGTC 
AGCTCAGGCA TGTGGTCTGC CATCTACTTC ATTGTGCTCA CCTTGTTTGG AAACTACACG 
CTGCTAAATG TATTCTTGGC CATCGCTGTG GACAATCTGG CCAATGCCCA GGAACTGACC 
AAGGATGAGC AGGAGGAAGA AGAAGCCTTC AACCAGAAAC ATGCACTGCA GAAGGCCAAG 
GAGGTCAGCC CGATGTCTGC TCCCAACATG CCTTCCATTG AAAGAGACAG AAGGAGAAGA 
CACCACATGT CGATGTGGGA GCCACGCAGC AGCCACCTGA GGGAGCGGAG GCGCCGGCAC 
CACATGTCTG TGTGGGAACA ACGCACCAGC CAGCTGAGGA GGCACATGCA AATGTCTAGC 
CAAGAAGCCC TCAACAAAGA GGAGGCCCCG CCAATGAACC CCCTCAATCC ACTCAACCCA 
CTGAGTCCTC TCAACCCCCT CAATGCTCAC CCCAGCCTTT ATCGGAGGCC CAGGCCCATT 
GAGGGCCTAG CCCTAGGTCT GGGTCTTGAA AAGTGTGAAG AAGAACGCAT CAGTCGTGGG 
GGCTCCCTCA AGGGGGACAT AGGGGGCCTG ACTAGCGCCC TGGACAACCA GAGGAGCCCC 
TTGTCCTTGG GCAAACGGGA GCCACCGTGG CTGCCTAGAT CCTGTCATGG AAACTGTGAC 
CCAATCCAGC AGGAGGCTGG GGGAGGAGAG ACTGTGGTGA CTTTTGAGGA CCGGGCCAGG 
CACAGGCAGA GTCAGAGGCG AAGCCGGCAT CGCCGAGTCA GGACTGAGGG CAAGGACTCT 
GCCTCTGCAT CCCGAAGCAG GTCTGCCAGC CAGGAGCGGA GTCTGGATGA AGGGGTGTCC 
GTTGAGGGGG AGAAGGAACA TGAGCCTCAC AGCAGCCACA GGAGCAAAGA GCCGACCATC 
CACGAAGAGG AGAGAACCCA GGACTTAAGG AGGACCAACA GTCTGATGGT CCCCAGGGGC 
TCTGGACTGG TAGGGGCTCT TGATGAAGCA GAAACACCTC TAGTCCAGCC CCAACCTGAG 
CTGGAAGTGG GGAAGGATGC AGCTCTGACA GAGCAGGAGG CTGAAGGCAG CAGTGAACAA 
GCCCTTCTTG GGGACGTACA GCTGGATGTG GGCCGGGGCA TTAGCCAGAG TGAACCGGAT 
CTCTCCTGCA TGACAGCCAA CATGGACAAG GCTACCACGG AGAGCACCAG TGTCACGGTC 
GCCATCCCTG ATGTGGACCC CTTGGTGGAC TCGACAGTGG TGAACATTAG CAACAAGACG 
GATGGAGAAG CCAGTCCCTT GAAGGAGGCA GAGACTAAAG AGGAAGAAGA AGAGGTAGAG 
AAGAAGAAGA AGCAGAAGAA GGAGAAGCGA GAGACTGGAA AAGCCATGGT GCCCCATAGC 
TCTATGTTCA TCTTCAGCAC CACTAACCCG ATCCGCAGGG CCTGCCACTA CATTGTGAAC 
CTACGCTACT TTGAAATGTG CATTCTCTTG GTGATTGCGG CCAGCAGCAT TGCCCTGGCA 
GCCGAGGACC CTGTACTAAC TAACTCGGAG CGTAACAAAG TCCTGAGATA TTTCGACTAT 
GTTTTCACAG GAGTGTTTAC CTTTGAGATG GTTATAAAGA TGATAGACCA AGGCTTGATC 
CTGCAGGATG GATCATACTT CAGAGACCTC TGGAACATCC TGGACTTTGT AGTAGTGGTT 
GGTGCATTGG TAGCATTTGC TCTGGCGAAC GCTTTGGGAA CCAACAAGGG GAGGGATATC 
AAGACTATCA AGTCTCTGAG GGTACTTCGA GTTCTGCGGC CACTGAAAAC CATCAAGCGC 
TTGCCCAAGC TGAAGGCTGT GTTTGACTGC GTGGTGACCT CCTTGAAGAA TGTCTTCAAC 
ATACTCATTG TCTACAAGCT CTTCATGTTC ATCTTTGCCG TCATCGCTGT TCAGCTATTC 
AAAGGAAAGT TCTTTTATTG CACTGACAGT TCCAAGGACA CAGAGAAGGA GTGCATAGGC 
AACTATGTAG ACCACGAGAA AAACAAGATG GAGGTGAAGG GCCGGGAATG GAAGCGTCAT 
GAATTCCACT ATGACAACAT CATCTGGGCC CTGCTTACCC TCTTCACAGT CTCTACAGGG 
GAAGGATGGC CTCAGGTTCT GCAGCACTCT GTAGATGTGA CAGAGGAGGA CAGAGGCCCA 
AGTCGCAGCA ACCGCATGGA GATGTCCATC TTTTATGTGG TCTACTTTGT GGTCTTCCCT 
TTCTTCTTTG TCAATATCTT CGTGGCTCTC ATTATCATCA CCTTCCAGGA GCAAGGGGAC 
AAGATGATGG AGGAGTGTAG CCTGGAGAAG AATGAGCGGG CATGCATCGA CTTCGCCATT 
AGTGCCAAAC CTCTCACCCG CTACATGCCA CAGAACAGGC ACACCTTCCA GTACCGTGTG 
TGGCACTTTG TAGTCTCCCC ATCCTTTGAG TATACCATCA TGGCCATGAT TGCCTTGAAC 
ACTGTCGTGC TGATGATGAA GTACTACTCT GCTCCTTGCA CCTATGAGCT CGCCTTAAAG 
TACCTGAACA TCGCCTTCAC TATGGTGTTT TCCCTGGAGT GTGTCCTAAA GGTCATTGCT 
TTCGGCTTCC TGAACTATTT CCGAGATACC TGGAACATCT TTGACTTCAT CACGGTGATC 
GGCAGTATCA CAGAAATTAT CCTGACAGAT AGCAAGCTGG TGAACACCAG TGGCTTCAAT 
ATGAGCTTTC TGAAGCTCTT CCGAGCTGCA CGGCTCATAA AGCTTCTGAG ACAGGGCTAC 
ACCATCCGCA TCTTACTGTG GACCTTCGTG CAGTCCTTTA AGGCTCTTCC CTACGTCTGC 
CTTCTCATTG CCATGCTTTT CTTCATCTAC GCCATCATTG GGATGCAGGT GTTTGGAAAC 
ATAAAGTTAG ATGAGGAGAG TCACATCAAC CGGCACAACA ACTTCCGGAG TTTCTTTGGA 
TCCCTCATGC TGCTTTTCAG GAGTGCCACG GGGGAGGCTT GGCAGGAGAT TATGCTGTCC 
TGTCTCGGTG AGAAGGGTTG TGAGCCTGAC ACCACTGCAC CCTCAGGGCA GAATGAGAGT 
GAGCGCTGTG GCACTGACCT GGCCTACGTT TATTTTGTCT CCTTCATCTT CTTCTGCTCC 
TTCCTGATGC TCAACCTGTT CGTGGCTGTC ATCATGGACA ACTTTGAGTA CCTGACTCGA 
GATTCCTCCA TCCTGGGGCC TCACCACTTA GATGAGTTTG TCCGTGTCTG GGCAGAATAT 
GACAGAGCAG CATGTGGCCG CATCCATTAC ACTGAGATGT ATGAAATGCT GACTCTCATG 
TCACCACCGC TAGGCCTCGG CAAGAGATGT CCCTCCAAAG TGGCCTATAA GAGGCTGGTC 
CTGATGAACA TGCCAGTGGC TGAGGACATG ACAGTCCACT TCACCTCCAC ACTTATGGCT 
CTGATCCGGA CAGCTCTGGA CATTAAAATC GCCAAAGGTG GTGCAGACAG ACAGCAGCTA 
GACTCGGAGC TGCAAAAAGA GACCCTGGCC ATTTGGCCTC ACCTGTCCCA AAAGATGTTG 
GATCTGCTTG TGCCCATGCC CAAAGCCTCT GACCTGACTG TGGGCAAGAT CTATGCAGCA 
ATGATGATCA TGGACTACTA TAAACAGAGT AAGGTAAAGA AACAGAGACA GCAGCTGGAA 
GAACAGAAAA ATGCACCCAT GTTCCAGCGC ATGGAGCCCT CATCACTGCC TCAGGAGATC 
ATTGCTAATG CCAAAGCCCT GCCTTATCTC CAGCAGGACC CTGTTTCTGG CCTGAGTGGT 
CGGAGTGGAT ACCCTTCAAT GAGTCCACTC TCCCCTCAAG AAATATTCCA GTTGGCTTGT 
ATGGACCCAG CGGATGATGG ACAATTCCAG GAACAACAAT CTCTGGTGAA TGCACAAGTG 
GTAACAGACC CTAGCTCTAT GCGACGTTCA TTTTCTACAA TTCGGGATAA GCGTTCAAAT 
TCCTCCTGGT TGGAGGAGTT CTCCATGGAA CGCAGCAGTG AAAACACTTA CAAGTCGCGG 
CGCAGAAGTT ACCATTCCTC CCTGAGGCTG TCAGCACACC GCCTGAATTC TGACTCTGGT 
CACAAGTCTG ACACTCACCG ATCAGGAGGC AGGGAGAGAG GCCGATCCAA AGAACGAAAG 
CACCTCCTCT CTCCAGATGT CTCCCGCTGC AATTCTGAAG AGCGAGGGAC CCAGGCCGAC 
TGGGAGTCCC CAGAACGTCG CCAGTCCAGG TCACCCAGTG AAGGAAGGTC ACAGACCCCC 
AATAGACAGG GTACCGGTTC CCTAAGTGAG AGCTCCATCC CCTCTATTTC CGACACCAGC 
ACCCCAAGAC GAAGCCGTCG CCAGCTCCCA CCTGTGCCAC CAAAGCCTCG TCCCCTCCTC 
TCCTACAGCT CCCTGATGAG ACACACTGGC GGCATCTCTC CACCTCCTGA TGGAAGTGAG 
GGTGGATCCC CACTGGCCTC TCAAGCTCTG GAGAGCAACA GCGCTTGCCT GACCGAGTCT 
TCCAACTCCT TGCACCCCCA GCAGGGCCAG CATCCTTCCC CACAGCACTA CATCTCCGAG 
CCCTATCTGG CCCTCCATGA AGACTCCCAC GCCTCAGACT GTGGCGAGGA GGAGACACTC 
ACCTTTGAGG CAGCCGTAGC CACTAGCTTG GGCCGGTCCA ATACCATTGG CTCTGCGCCA 
CCCCTACGGC ACAGCTGGCA GATGCCTAAT GGGCACTATC GGCGGCGGAG GCGTGGGGGG 
CCTGGGCCTG GCATGATGTG TGGAGCTGTC AGTGACCTCC TGAGTGACAC GGAAGAAGAC 
GATAAGTGCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017168219.1
CDS	594..7865
Translation	MVPEMRGLSPGRRLPEPTLCPATGGSPEPGAAGRRRHVLALPDEEAWRPQQCALLEANASDEVGMLPASPRSAVAGFDNFFPVQEGEGPGWEGSTTLDAGSSPFLPVSPEVMKRRRGGLIEQRDIIKAHEAHKMQSTPQARRKEWEMARFGEAVVVGRPGSGDGDSDQSRNRQGTPVPASGPAAAYKQSKAQRARTMALYNPIPVRQNCFTVNRSLFIFGEDNIVRKYAKKLIDWPPFEYMILATIIANCIVLALEQHLPEDDKTPMSRRLEKTEPYFIGIFCFEAGIKIVALGFIFHKGSYLRNGWNVMDFIVVLSGILATAGTHFNTHVDLRTLRAVRVLRPLKLVSGIPSLQIVLKSIMKAMVPLLQIGLLLFFAILMFAIIGLEFYSGKLHRACFMNNSGILEGFDPPHPCGVQGCPAGYECKDWIGPNDGITQFDNILFAVLTVFQCITMEGWTTVLYNTNDALGATWNWLYFIPLIIIGSFFVLNLVLGVLSGEFAKERERVENRRAFMKLRRQQQIERELNGYRAWIDKAEEVMLAEENKNSGTSALEVLRRATIKRSRTEAMTRDSSDEHCVDISSVGTPLARASIKSTKVDGASYFRHKERLLRISIRHMVKSQVFYWIVLSVVALNTACVAIVHHNQPQWLTHLLYYAEFLFLGLFLLEMSLKMYGMGPRLYFHSSFNCFDFGVTVGSIFEVVWAIFRPGTSFGISVLRALRLLRIFKITKYWASLRNLVVSLMSSMKSIISLLFLLFLFIVVFALLGMQLFGGRFNFNDGTPSANFDTFPAAIMTVFQILTGEDWNEVMYNGIRSQGGVSSGMWSAIYFIVLTLFGNYTLLNVFLAIAVDNLANAQELTKDEQEEEEAFNQKHALQKAKEVSPMSAPNMPSIERDRRRRHHMSMWEPRSSHLRERRRRHHMSVWEQRTSQLRRHMQMSSQEALNKEEAPPMNPLNPLNPLSPLNPLNAHPSLYRRPRPIEGLALGLGLEKCEEERISRGGSLKGDIGGLTSALDNQRSPLSLGKREPPWLPRSCHGNCDPIQQEAGGGETVVTFEDRARHRQSQRRSRHRRVRTEGKDSASASRSRSASQERSLDEGVSVEGEKEHEPHSSHRSKEPTIHEEERTQDLRRTNSLMVPRGSGLVGALDEAETPLVQPQPELEVGKDAALTEQEAEGSSEQALLGDVQLDVGRGISQSEPDLSCMTANMDKATTESTSVTVAIPDVDPLVDSTVVNISNKTDGEASPLKEAETKEEEEEVEKKKKQKKEKRETGKAMVPHSSMFIFSTTNPIRRACHYIVNLRYFEMCILLVIAASSIALAAEDPVLTNSERNKVLRYFDYVFTGVFTFEMVIKMIDQGLILQDGSYFRDLWNILDFVVVVGALVAFALANALGTNKGRDIKTIKSLRVLRVLRPLKTIKRLPKLKAVFDCVVTSLKNVFNILIVYKLFMFIFAVIAVQLFKGKFFYCTDSSKDTEKECIGNYVDHEKNKMEVKGREWKRHEFHYDNIIWALLTLFTVSTGEGWPQVLQHSVDVTEEDRGPSRSNRMEMSIFYVVYFVVFPFFFVNIFVALIIITFQEQGDKMMEECSLEKNERACIDFAISAKPLTRYMPQNRHTFQYRVWHFVVSPSFEYTIMAMIALNTVVLMMKYYSAPCTYELALKYLNIAFTMVFSLECVLKVIAFGFLNYFRDTWNIFDFITVIGSITEIILTDSKLVNTSGFNMSFLKLFRAARLIKLLRQGYTIRILLWTFVQSFKALPYVCLLIAMLFFIYAIIGMQVFGNIKLDEESHINRHNNFRSFFGSLMLLFRSATGEAWQEIMLSCLGEKGCEPDTTAPSGQNESERCGTDLAYVYFVSFIFFCSFLMLNLFVAVIMDNFEYLTRDSSILGPHHLDEFVRVWAEYDRAACGRIHYTEMYEMLTLMSPPLGLGKRCPSKVAYKRLVLMNMPVAEDMTVHFTSTLMALIRTALDIKIAKGGADRQQLDSELQKETLAIWPHLSQKMLDLLVPMPKASDLTVGKIYAAMMIMDYYKQSKVKKQRQQLEEQKNAPMFQRMEPSSLPQEIIANAKALPYLQQDPVSGLSGRSGYPSMSPLSPQEIFQLACMDPADDGQFQEQQSLVNAQVVTDPSSMRRSFSTIRDKRSNSSWLEEFSMERSSENTYKSRRRSYHSSLRLSAHRLNSDSGHKSDTHRSGGRERGRSKERKHLLSPDVSRCNSEERGTQADWESPERRQSRSPSEGRSQTPNRQGTGSLSESSIPSISDTSTPRRSRRQLPPVPPKPRPLLSYSSLMRHTGGISPPPDGSEGGSPLASQALESNSACLTESSNSLHPQQGQHPSPQHYISEPYLALHEDSHASDCGEEETLTFEAAVATSLGRSNTIGSAPPLRHSWQMPNGHYRRRRRGGPGPGMMCGAVSDLLSDTEEDDKC

Target ORF information:

RefSeq Version	XM_017312730.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus calcium channel, voltage-dependent, R type, alpha 1E subunit (Cacna1e), transcript variant X12, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017312730.1

ORF Insert Sequence:

ATGGTGCCCG AGATGCGTGG ACTGAGCCCA GGCCGAAGGC TGCCCGAGCC CACCCTGTGC 
CCGGCTACAG GAGGGAGCCC TGAGCCCGGG GCTGCTGGCA GGCGCCGCCA CGTCCTGGCT 
CTGCCTGATG AGGAGGCATG GCGGCCGCAG CAGTGCGCCC TGCTCGAGGC CAATGCCTCC 
GACGAGGTGG GCATGTTGCC TGCTTCCCCG CGCTCCGCGG TGGCCGGCTT CGACAACTTC 
TTCCCGGTGC AGGAAGGCGA GGGCCCGGGC TGGGAGGGCA GCACCACGCT GGATGCAGGC 
TCCAGCCCCT TCCTTCCTGT GAGCCCAGAG GTCATGAAGC GGAGGCGAGG GGGGCTCATC 
GAGCAGCGGG ACATCATCAA GGCCCACGAG GCACACAAGA TGCAGAGCAC CCCGCAGGCC 
CGGCGCAAGG AGTGGGAGAT GGCTCGCTTC GGGGAGGCGG TGGTCGTTGG CAGGCCAGGC 
TCAGGCGATG GAGACTCGGA CCAGAGCAGG AACCGACAAG GAACCCCCGT CCCGGCCTCG 
GGGCCGGCGG CCGCCTACAA GCAGTCAAAA GCGCAGAGGG CGCGGACTAT GGCTTTGTAC 
AACCCCATAC CAGTCCGGCA GAACTGTTTC ACGGTCAACA GATCCCTGTT CATCTTCGGA 
GAAGATAACA TTGTCAGGAA ATACGCCAAG AAGCTCATCG ATTGGCCGCC ATTTGAGTAC 
ATGATCCTGG CCACCATCAT TGCCAACTGC ATCGTCTTGG CCCTGGAGCA GCATCTTCCT 
GAGGATGACA AGACCCCAAT GTCTCGAAGA CTGGAGAAGA CAGAACCATA TTTCATTGGG 
ATCTTCTGCT TTGAAGCTGG GATCAAAATT GTGGCTCTAG GGTTCATCTT CCATAAGGGT 
TCATACCTCC GTAATGGCTG GAATGTCATG GACTTCATCG TGGTCCTTAG TGGCATCCTG 
GCCACTGCGG GAACCCACTT CAACACCCAC GTGGACCTGC GGACCCTCCG GGCTGTGCGT 
GTCCTGAGGC CTTTAAAGCT GGTATCAGGA ATACCTAGCC TGCAGATCGT GCTGAAGTCT 
ATCATGAAGG CCATGGTGCC TCTTCTGCAG ATTGGCCTTC TGCTCTTCTT TGCCATCCTC 
ATGTTTGCTA TCATTGGCTT GGAGTTCTAC AGTGGCAAGT TGCATCGAGC ATGCTTCATG 
AACAATTCAG GTATTCTAGA GGGGTTTGAT CCTCCTCACC CGTGTGGTGT GCAAGGCTGC 
CCGGCTGGTT ATGAATGTAA GGACTGGATC GGCCCCAATG ACGGGATCAC CCAGTTTGAC 
AACATCCTTT TTGCCGTGCT GACTGTCTTC CAATGCATCA CTATGGAAGG GTGGACCACT 
GTGCTGTACA ATACCAATGA TGCCTTAGGA GCCACCTGGA ACTGGCTGTA CTTCATCCCT 
CTCATCATCA TCGGATCCTT CTTTGTTCTC AACCTCGTCC TGGGAGTGCT TTCCGGGGAA 
TTTGCCAAAG AAAGAGAGAG AGTGGAGAAC CGAAGAGCGT TCATGAAGCT CCGGCGCCAG 
CAACAGATTG AGCGAGAATT GAACGGCTAC CGTGCCTGGA TAGACAAAGC AGAGGAAGTC 
ATGCTTGCTG AAGAAAATAA AAACTCGGGA ACATCAGCCT TGGAAGTGCT TCGAAGGGCA 
ACCATCAAAA GGAGCCGGAC GGAGGCCATG ACCCGAGACT CCAGCGATGA GCACTGCGTT 
GATATCTCTT CAGTGGGCAC ACCTCTTGCC AGAGCCAGTA TCAAGAGCAC AAAAGTAGAT 
GGGGCCTCCT ATTTCCGGCA CAAGGAACGG CTTCTGCGCA TCTCTATCCG CCACATGGTC 
AAGTCCCAAG TCTTTTACTG GATCGTCCTG AGTGTTGTGG CTCTTAATAC TGCCTGTGTG 
GCCATTGTTC ATCACAACCA GCCACAGTGG CTCACTCACC TCCTCTACTA TGCAGAGTTT 
TTGTTTCTGG GACTCTTCCT GTTGGAGATG TCCCTGAAGA TGTATGGCAT GGGGCCACGC 
CTCTATTTTC ACTCATCCTT CAACTGCTTT GATTTTGGGG TCACAGTGGG CAGCATCTTT 
GAGGTTGTCT GGGCAATCTT CAGACCTGGC ACATCTTTTG GAATCAGTGT CTTACGAGCT 
CTCCGGCTCC TAAGGATATT TAAAATAACC AAGTATTGGG CATCCCTCCG GAATCTGGTT 
GTCTCCTTGA TGAGCTCCAT GAAGTCTATC ATCAGCTTAC TCTTCCTTCT CTTCCTCTTC 
ATCGTTGTCT TTGCTCTCCT GGGAATGCAG CTCTTTGGAG GCAGGTTTAA CTTTAATGAT 
GGGACTCCTT CAGCTAATTT TGACACCTTC CCTGCAGCTA TCATGACAGT GTTCCAGATC 
CTGACAGGGG AAGACTGGAA TGAGGTGATG TACAATGGCA TTCGCTCCCA GGGTGGGGTC 
AGCTCAGGCA TGTGGTCTGC CATCTACTTC ATTGTGCTCA CCTTGTTTGG AAACTACACG 
CTGCTAAATG TATTCTTGGC CATCGCTGTG GACAATCTGG CCAATGCCCA GGAACTGACC 
AAGGATGAGC AGGAGGAAGA AGAAGCCTTC AACCAGAAAC ATGCACTGCA GAAGGCCAAG 
GAGGTCAGCC CGATGTCTGC TCCCAACATG CCTTCCATTG AAAGAGACAG AAGGAGAAGA 
CACCACATGT CGATGTGGGA GCCACGCAGC AGCCACCTGA GGGAGCGGAG GCGCCGGCAC 
CACATGTCTG TGTGGGAACA ACGCACCAGC CAGCTGAGGA GGCACATGCA AATGTCTAGC 
CAAGAAGCCC TCAACAAAGA GGAGGCCCCG CCAATGAACC CCCTCAATCC ACTCAACCCA 
CTGAGTCCTC TCAACCCCCT CAATGCTCAC CCCAGCCTTT ATCGGAGGCC CAGGCCCATT 
GAGGGCCTAG CCCTAGGTCT GGGTCTTGAA AAGTGTGAAG AAGAACGCAT CAGTCGTGGG 
GGCTCCCTCA AGGGGGACAT AGGGGGCCTG ACTAGCGCCC TGGACAACCA GAGGAGCCCC 
TTGTCCTTGG GCAAACGGGA GCCACCGTGG CTGCCTAGAT CCTGTCATGG AAACTGTGAC 
CCAATCCAGC AGGAGGCTGG GGGAGGAGAG ACTGTGGTGA CTTTTGAGGA CCGGGCCAGG 
CACAGGCAGA GTCAGAGGCG AAGCCGGCAT CGCCGAGTCA GGACTGAGGG CAAGGACTCT 
GCCTCTGCAT CCCGAAGCAG GTCTGCCAGC CAGGAGCGGA GTCTGGATGA AGGGGTGTCC 
GTTGAGGGGG AGAAGGAACA TGAGCCTCAC AGCAGCCACA GGAGCAAAGA GCCGACCATC 
CACGAAGAGG AGAGAACCCA GGACTTAAGG AGGACCAACA GTCTGATGGT CCCCAGGGGC 
TCTGGACTGG TAGGGGCTCT TGATGAAGCA GAAACACCTC TAGTCCAGCC CCAACCTGAG 
CTGGAAGTGG GGAAGGATGC AGCTCTGACA GAGCAGGAGG CTGAAGGCAG CAGTGAACAA 
GCCCTTCTTG GGGACGTACA GCTGGATGTG GGCCGGGGCA TTAGCCAGAG TGAACCGGAT 
CTCTCCTGCA TGACAGCCAA CATGGACAAG GCTACCACGG AGAGCACCAG TGTCACGGTC 
GCCATCCCTG ATGTGGACCC CTTGGTGGAC TCGACAGTGG TGAACATTAG CAACAAGACG 
GATGGAGAAG CCAGTCCCTT GAAGGAGGCA GAGACTAAAG AGGAAGAAGA AGAGGTAGAG 
AAGAAGAAGA AGCAGAAGAA GGAGAAGCGA GAGACTGGAA AAGCCATGGT GCCCCATAGC 
TCTATGTTCA TCTTCAGCAC CACTAACCCG ATCCGCAGGG CCTGCCACTA CATTGTGAAC 
CTACGCTACT TTGAAATGTG CATTCTCTTG GTGATTGCGG CCAGCAGCAT TGCCCTGGCA 
GCCGAGGACC CTGTACTAAC TAACTCGGAG CGTAACAAAG TCCTGAGATA TTTCGACTAT 
GTTTTCACAG GAGTGTTTAC CTTTGAGATG GTTATAAAGA TGATAGACCA AGGCTTGATC 
CTGCAGGATG GATCATACTT CAGAGACCTC TGGAACATCC TGGACTTTGT AGTAGTGGTT 
GGTGCATTGG TAGCATTTGC TCTGGCGAAC GCTTTGGGAA CCAACAAGGG GAGGGATATC 
AAGACTATCA AGTCTCTGAG GGTACTTCGA GTTCTGCGGC CACTGAAAAC CATCAAGCGC 
TTGCCCAAGC TGAAGGCTGT GTTTGACTGC GTGGTGACCT CCTTGAAGAA TGTCTTCAAC 
ATACTCATTG TCTACAAGCT CTTCATGTTC ATCTTTGCCG TCATCGCTGT TCAGCTATTC 
AAAGGAAAGT TCTTTTATTG CACTGACAGT TCCAAGGACA CAGAGAAGGA GTGCATAGGC 
AACTATGTAG ACCACGAGAA AAACAAGATG GAGGTGAAGG GCCGGGAATG GAAGCGTCAT 
GAATTCCACT ATGACAACAT CATCTGGGCC CTGCTTACCC TCTTCACAGT CTCTACAGGG 
GAAGGATGGC CTCAGGTTCT GCAGCACTCT GTAGATGTGA CAGAGGAGGA CAGAGGCCCA 
AGTCGCAGCA ACCGCATGGA GATGTCCATC TTTTATGTGG TCTACTTTGT GGTCTTCCCT 
TTCTTCTTTG TCAATATCTT CGTGGCTCTC ATTATCATCA CCTTCCAGGA GCAAGGGGAC 
AAGATGATGG AGGAGTGTAG CCTGGAGAAG AATGAGCGGG CATGCATCGA CTTCGCCATT 
AGTGCCAAAC CTCTCACCCG CTACATGCCA CAGAACAGGC ACACCTTCCA GTACCGTGTG 
TGGCACTTTG TAGTCTCCCC ATCCTTTGAG TATACCATCA TGGCCATGAT TGCCTTGAAC 
ACTGTCGTGC TGATGATGAA GTACTACTCT GCTCCTTGCA CCTATGAGCT CGCCTTAAAG 
TACCTGAACA TCGCCTTCAC TATGGTGTTT TCCCTGGAGT GTGTCCTAAA GGTCATTGCT 
TTCGGCTTCC TGAACTATTT CCGAGATACC TGGAACATCT TTGACTTCAT CACGGTGATC 
GGCAGTATCA CAGAAATTAT CCTGACAGAT AGCAAGCTGG TGAACACCAG TGGCTTCAAT 
ATGAGCTTTC TGAAGCTCTT CCGAGCTGCA CGGCTCATAA AGCTTCTGAG ACAGGGCTAC 
ACCATCCGCA TCTTACTGTG GACCTTCGTG CAGTCCTTTA AGGCTCTTCC CTACGTCTGC 
CTTCTCATTG CCATGCTTTT CTTCATCTAC GCCATCATTG GGATGCAGGT GTTTGGAAAC 
ATAAAGTTAG ATGAGGAGAG TCACATCAAC CGGCACAACA ACTTCCGGAG TTTCTTTGGA 
TCCCTCATGC TGCTTTTCAG GAGTGCCACG GGGGAGGCTT GGCAGGAGAT TATGCTGTCC 
TGTCTCGGTG AGAAGGGTTG TGAGCCTGAC ACCACTGCAC CCTCAGGGCA GAATGAGAGT 
GAGCGCTGTG GCACTGACCT GGCCTACGTT TATTTTGTCT CCTTCATCTT CTTCTGCTCC 
TTCCTGATGC TCAACCTGTT CGTGGCTGTC ATCATGGACA ACTTTGAGTA CCTGACTCGA 
GATTCCTCCA TCCTGGGGCC TCACCACTTA GATGAGTTTG TCCGTGTCTG GGCAGAATAT 
GACAGAGCAG CATGTGGCCG CATCCATTAC ACTGAGATGT ATGAAATGCT GACTCTCATG 
TCACCACCGC TAGGCCTCGG CAAGAGATGT CCCTCCAAAG TGGCCTATAA GAGGCTGGTC 
CTGATGAACA TGCCAGTGGC TGAGGACATG ACAGTCCACT TCACCTCCAC ACTTATGGCT 
CTGATCCGGA CAGCTCTGGA CATTAAAATC GCCAAAGGTG GTGCAGACAG ACAGCAGCTA 
GACTCGGAGC TGCAAAAAGA GACCCTGGCC ATTTGGCCTC ACCTGTCCCA AAAGATGTTG 
GATCTGCTTG TGCCCATGCC CAAAGCCTCT GACCTGACTG TGGGCAAGAT CTATGCAGCA 
ATGATGATCA TGGACTACTA TAAACAGAGT AAGGTAAAGA AACAGAGACA GCAGCTGGAA 
GAACAGAAAA ATGCACCCAT GTTCCAGCGC ATGGAGCCCT CATCACTGCC TCAGGAGATC 
ATTGCTAATG CCAAAGCCCT GCCTTATCTC CAGCAGGACC CTGTTTCTGG CCTGAGTGGT 
CGGAGTGGAT ACCCTTCAAT GAGTCCACTC TCCCCTCAAG AAATATTCCA GTTGGCTTGT 
ATGGACCCAG CGGATGATGG ACAATTCCAG GAACAACAAT CTCTGGTGAA TGCACAAGTG 
GTAACAGACC CTAGCTCTAT GCGACGTTCA TTTTCTACAA TTCGGGATAA GCGTTCAAAT 
TCCTCCTGGT TGGAGGAGTT CTCCATGGAA CGCAGCAGTG AAAACACTTA CAAGTCGCGG 
CGCAGAAGTT ACCATTCCTC CCTGAGGCTG TCAGCACACC GCCTGAATTC TGACTCTGGT 
CACAAGTCTG ACACTCACCG ATCAGGAGGC AGGGAGAGAG GCCGATCCAA AGAACGAAAG 
CACCTCCTCT CTCCAGATGT CTCCCGCTGC AATTCTGAAG AGCGAGGGAC CCAGGCCGAC 
TGGGAGTCCC CAGAACGTCG CCAGTCCAGG TCACCCAGTG AAGGAAGGTC ACAGACCCCC 
AATAGACAGG GTACCGGTTC CCTAAGTGAG AGCTCCATCC CCTCTATTTC CGACACCAGC 
ACCCCAAGAC GAAGCCGTCG CCAGCTCCCA CCTGTGCCAC CAAAGCCTCG TCCCCTCCTC 
TCCTACAGCT CCCTGATGAG ACACACTGGC GGCATCTCTC CACCTCCTGA TGGAAGTGAG 
GGTGGATCCC CACTGGCCTC TCAAGCTCTG GAGAGCAACA GCGCTTGCCT GACCGAGTCT 
TCCAACTCCT TGCACCCCCA GCAGGGCCAG CATCCTTCCC CACAGCACTA CATCTCCGAG 
CCCTATCTGG CCCTCCATGA AGACTCCCAC GCCTCAGACT GTGGCGAGGA GGAGACACTC 
ACCTTTGAGG CAGCCGTAGC CACTAGCTTG GGCCGGTCCA ATACCATTGG CTCTGCGCCA 
CCCCTACGGC ACAGCTGGCA GATGCCTAAT GGGCACTATC GGCGGCGGAG GCGTGGGGGG 
CCTGGGCCTG GCATGATGTG TGGAGCTGTC AGTGACCTCC TGAGTGACAC GGAAGAAGAC 
GATAAGTGCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cacna1e ( XM_011247916.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011246218.1 Mus musculus calcium channel, voltage-dependent, R type, alpha 1E subunit (Cacna1e), transcript variant X16, mRNA.

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CloneID	OMu67267
Clone ID Related Accession (Same CDS sequence)	XM_011247916.3 , XM_011247916.2 , XM_017312848.1
Accession Version	XM_011247916.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6963bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	voltage-dependent R-type calcium channel subunit alpha-1E isoform X14
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_011247916.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTCGCT TCGGGGAGGC GGTGGTCGTT GGCAGGCCAG GCTCAGGCGA TGGAGACTCG 
GACCAGAGCA GGAACCGACA AGGAACCCCC GTCCCGGCCT CGGGGCCGGC GGCCGCCTAC 
AAGCAGTCAA AAGCGCAGAG GGCGCGGACT ATGGCTTTGT ACAACCCCAT ACCAGTCCGG 
CAGAACTGTT TCACGGTCAA CAGATCCCTG TTCATCTTCG GAGAAGATAA CATTGTCAGG 
AAATACGCCA AGAAGCTCAT CGATTGGCCG CCATTTGAGT ACATGATCCT GGCCACCATC 
ATTGCCAACT GCATCGTCTT GGCCCTGGAG CAGCATCTTC CTGAGGATGA CAAGACCCCA 
ATGTCTCGAA GACTGGAGAA GACAGAACCA TATTTCATTG GGATCTTCTG CTTTGAAGCT 
GGGATCAAAA TTGTGGCTCT AGGGTTCATC TTCCATAAGG GTTCATACCT CCGTAATGGC 
TGGAATGTCA TGGACTTCAT CGTGGTCCTT AGTGGCATCC TGGCCACTGC GGGAACCCAC 
TTCAACACCC ACGTGGACCT GCGGACCCTC CGGGCTGTGC GTGTCCTGAG GCCTTTAAAG 
CTGGTATCAG GAATACCTAG CCTGCAGATC GTGCTGAAGT CTATCATGAA GGCCATGGTG 
CCTCTTCTGC AGATTGGCCT TCTGCTCTTC TTTGCCATCC TCATGTTTGC TATCATTGGC 
TTGGAGTTCT ACAGTGGCAA GTTGCATCGA GCATGCTTCA TGAACAATTC AGGTATTCTA 
GAGGGGTTTG ATCCTCCTCA CCCGTGTGGT GTGCAAGGCT GCCCGGCTGG TTATGAATGT 
AAGGACTGGA TCGGCCCCAA TGACGGGATC ACCCAGTTTG ACAACATCCT TTTTGCCGTG 
CTGACTGTCT TCCAATGCAT CACTATGGAA GGGTGGACCA CTGTGCTGTA CAATACCAAT 
GATGCCTTAG GAGCCACCTG GAACTGGCTG TACTTCATCC CTCTCATCAT CATCGGATCC 
TTCTTTGTTC TCAACCTCGT CCTGGGAGTG CTTTCCGGGG AATTTGCCAA AGAAAGAGAG 
AGAGTGGAGA ACCGAAGAGC GTTCATGAAG CTCCGGCGCC AGCAACAGAT TGAGCGAGAA 
TTGAACGGCT ACCGTGCCTG GATAGACAAA GCAGAGGAAG TCATGCTTGC TGAAGAAAAT 
AAAAACTCGG GAACATCAGC CTTGGAAGTG CTTCGAAGGG CAACCATCAA AAGGAGCCGG 
ACGGAGGCCA TGACCCGAGA CTCCAGCGAT GAGCACTGCG TTGATATCTC TTCAGTGGGC 
ACACCTCTTG CCAGAGCCAG TATCAAGAGC ACAAAAGTAG ATGGGGCCTC CTATTTCCGG 
CACAAGGAAC GGCTTCTGCG CATCTCTATC CGCCACATGG TCAAGTCCCA AGTCTTTTAC 
TGGATCGTCC TGAGTGTTGT GGCTCTTAAT ACTGCCTGTG TGGCCATTGT TCATCACAAC 
CAGCCACAGT GGCTCACTCA CCTCCTCTAC TATGCAGAGT TTTTGTTTCT GGGACTCTTC 
CTGTTGGAGA TGTCCCTGAA GATGTATGGC ATGGGGCCAC GCCTCTATTT TCACTCATCC 
TTCAACTGCT TTGATTTTGG GGTCACAGTG GGCAGCATCT TTGAGGTTGT CTGGGCAATC 
TTCAGACCTG GCACATCTTT TGGAATCAGT GTCTTACGAG CTCTCCGGCT CCTAAGGATA 
TTTAAAATAA CCAAGTATTG GGCATCCCTC CGGAATCTGG TTGTCTCCTT GATGAGCTCC 
ATGAAGTCTA TCATCAGCTT ACTCTTCCTT CTCTTCCTCT TCATCGTTGT CTTTGCTCTC 
CTGGGAATGC AGCTCTTTGG AGGCAGGTTT AACTTTAATG ATGGGACTCC TTCAGCTAAT 
TTTGACACCT TCCCTGCAGC TATCATGACA GTGTTCCAGA TCCTGACAGG GGAAGACTGG 
AATGAGGTGA TGTACAATGG CATTCGCTCC CAGGGTGGGG TCAGCTCAGG CATGTGGTCT 
GCCATCTACT TCATTGTGCT CACCTTGTTT GGAAACTACA CGCTGCTAAA TGTATTCTTG 
GCCATCGCTG TGGACAATCT GGCCAATGCC CAGGAACTGA CCAAGGATGA GCAGGAGGAA 
GAAGAAGCCT TCAACCAGAA ACATGCACTG CAGAAGGCCA AGGAGGTCAG CCCGATGTCT 
GCTCCCAACA TGCCTTCCAT TGAAAGAGAC AGAAGGAGAA GACACCACAT GTCGATGTGG 
GAGCCACGCA GCAGCCACCT GAGGGAGCGG AGGCGCCGGC ACCACATGTC TGTGTGGGAA 
CAACGCACCA GCCAGCTGAG GAGGCACATG CAAATGTCTA GCCAAGAAGC CCTCAACAAA 
GAGGAGGCCC CGCCAATGAA CCCCCTCAAT CCACTCAACC CACTGAGTCC TCTCAACCCC 
CTCAATGCTC ACCCCAGCCT TTATCGGAGG CCCAGGCCCA TTGAGGGCCT AGCCCTAGGT 
CTGGGTCTTG AAAAGTGTGA AGAAGAACGC ATCAGTCGTG GGGGCTCCCT CAAGGGGGAC 
ATAGGGGGCC TGACTAGCGC CCTGGACAAC CAGAGGAGCC CCTTGTCCTT GGGCAAACGG 
GAGCCACCGT GGCTGCCTAG ATCCTGTCAT GGAAACTGTG ACCCAATCCA GCAGGAGGCT 
GGGGGAGGAG AGACTGTGGT GACTTTTGAG GACCGGGCCA GGCACAGGCA GAGTCAGAGG 
CGAAGCCGGC ATCGCCGAGT CAGGACTGAG GGCAAGGACT CTGCCTCTGC ATCCCGAAGC 
AGGTCTGCCA GCCAGGAGCG GAGTCTGGAT GAAGGGGTGT CCGTTGAGGG GGAGAAGGAA 
CATGAGCCTC ACAGCAGCCA CAGGAGCAAA GAGCCGACCA TCCACGAAGA GGAGAGAACC 
CAGGACTTAA GGAGGACCAA CAGTCTGATG GTCCCCAGGG GCTCTGGACT GGTAGGGGCT 
CTTGATGAAG CAGAAACACC TCTAGTCCAG CCCCAACCTG AGCTGGAAGT GGGGAAGGAT 
GCAGCTCTGA CAGAGCAGGA GGCTGAAGGC AGCAGTGAAC AAGCCCTTCT TGGGGACGTA 
CAGCTGGATG TGGGCCGGGG CATTAGCCAG AGTGAACCGG ATCTCTCCTG CATGACAGCC 
AACATGGACA AGGCTACCAC GGAGAGCACC AGTGTCACGG TCGCCATCCC TGATGTGGAC 
CCCTTGGTGG ACTCGACAGT GGTGAACATT AGCAACAAGA CGGATGGAGA AGCCAGTCCC 
TTGAAGGAGG CAGAGACTAA AGAGGAAGAA GAAGAGGTAG AGAAGAAGAA GAAGCAGAAG 
AAGGAGAAGC GAGAGACTGG AAAAGCCATG GTGCCCCATA GCTCTATGTT CATCTTCAGC 
ACCACTAACC CGATCCGCAG GGCCTGCCAC TACATTGTGA ACCTACGCTA CTTTGAAATG 
TGCATTCTCT TGGTGATTGC GGCCAGCAGC ATTGCCCTGG CAGCCGAGGA CCCTGTACTA 
ACTAACTCGG AGCGTAACAA AGTCCTGAGA TATTTCGACT ATGTTTTCAC AGGAGTGTTT 
ACCTTTGAGA TGGTTATAAA GATGATAGAC CAAGGCTTGA TCCTGCAGGA TGGATCATAC 
TTCAGAGACC TCTGGAACAT CCTGGACTTT GTAGTAGTGG TTGGTGCATT GGTAGCATTT 
GCTCTGGCGA ACGCTTTGGG AACCAACAAG GGGAGGGATA TCAAGACTAT CAAGTCTCTG 
AGGGTACTTC GAGTTCTGCG GCCACTGAAA ACCATCAAGC GCTTGCCCAA GCTGAAGGCT 
GTGTTTGACT GCGTGGTGAC CTCCTTGAAG AATGTCTTCA ACATACTCAT TGTCTACAAG 
CTCTTCATGT TCATCTTTGC CGTCATCGCT GTTCAGCTAT TCAAAGGAAA GTTCTTTTAT 
TGCACTGACA GTTCCAAGGA CACAGAGAAG GAGTGCATAG GCAACTATGT AGACCACGAG 
AAAAACAAGA TGGAGGTGAA GGGCCGGGAA TGGAAGCGTC ATGAATTCCA CTATGACAAC 
ATCATCTGGG CCCTGCTTAC CCTCTTCACA GTCTCTACAG GGGAAGGATG GCCTCAGGTT 
CTGCAGCACT CTGTAGATGT GACAGAGGAG GACAGAGGCC CAAGTCGCAG CAACCGCATG 
GAGATGTCCA TCTTTTATGT GGTCTACTTT GTGGTCTTCC CTTTCTTCTT TGTCAATATC 
TTCGTGGCTC TCATTATCAT CACCTTCCAG GAGCAAGGGG ACAAGATGAT GGAGGAGTGT 
AGCCTGGAGA AGAATGAGCG GGCATGCATC GACTTCGCCA TTAGTGCCAA ACCTCTCACC 
CGCTACATGC CACAGAACAG GCACACCTTC CAGTACCGTG TGTGGCACTT TGTAGTCTCC 
CCATCCTTTG AGTATACCAT CATGGCCATG ATTGCCTTGA ACACTGTCGT GCTGATGATG 
AAGTACTACT CTGCTCCTTG CACCTATGAG CTCGCCTTAA AGTACCTGAA CATCGCCTTC 
ACTATGGTGT TTTCCCTGGA GTGTGTCCTA AAGGTCATTG CTTTCGGCTT CCTGAACTAT 
TTCCGAGATA CCTGGAACAT CTTTGACTTC ATCACGGTGA TCGGCAGTAT CACAGAAATT 
ATCCTGACAG ATAGCAAGCT GGTGAACACC AGTGGCTTCA ATATGAGCTT TCTGAAGCTC 
TTCCGAGCTG CACGGCTCAT AAAGCTTCTG AGACAGGGCT ACACCATCCG CATCTTACTG 
TGGACCTTCG TGCAGTCCTT TAAGGCTCTT CCCTACGTCT GCCTTCTCAT TGCCATGCTT 
TTCTTCATCT ACGCCATCAT TGGGATGCAG GTGTTTGGAA ACATAAAGTT AGATGAGGAG 
AGTCACATCA ACCGGCACAA CAACTTCCGG AGTTTCTTTG GATCCCTCAT GCTGCTTTTC 
AGGAGTGCCA CGGGGGAGGC TTGGCAGGAG ATTATGCTGT CCTGTCTCGG TGAGAAGGGT 
TGTGAGCCTG ACACCACTGC ACCCTCAGGG CAGAATGAGA GTGAGCGCTG TGGCACTGAC 
CTGGCCTACG TTTATTTTGT CTCCTTCATC TTCTTCTGCT CCTTCCTGAT GCTCAACCTG 
TTCGTGGCTG TCATCATGGA CAACTTTGAG TACCTGACTC GAGATTCCTC CATCCTGGGG 
CCTCACCACT TAGATGAGTT TGTCCGTGTC TGGGCAGAAT ATGACAGAGC AGCATGTGGC 
CGCATCCATT ACACTGAGAT GTATGAAATG CTGACTCTCA TGTCACCACC GCTAGGCCTC 
GGCAAGAGAT GTCCCTCCAA AGTGGCCTAT AAGAGGCTGG TCCTGATGAA CATGCCAGTG 
GCTGAGGACA TGACAGTCCA CTTCACCTCC ACACTTATGG CTCTGATCCG GACAGCTCTG 
GACATTAAAA TCGCCAAAGG TGGTGCAGAC AGACAGCAGC TAGACTCGGA GCTGCAAAAA 
GAGACCCTGG CCATTTGGCC TCACCTGTCC CAAAAGATGT TGGATCTGCT TGTGCCCATG 
CCCAAAGCCT CTGACCTGAC TGTGGGCAAG ATCTATGCAG CAATGATGAT CATGGACTAC 
TATAAACAGA GTAAGGTAAA GAAACAGAGA CAGCAGCTGG AAGAACAGAA AAATGCACCC 
ATGTTCCAGC GCATGGAGCC CTCATCACTG CCTCAGGAGA TCATTGCTAA TGCCAAAGCC 
CTGCCTTATC TCCAGCAGGA CCCTGTTTCT GGCCTGAGTG GTCGGAGTGG ATACCCTTCA 
ATGAGTCCAC TCTCCCCTCA AGAAATATTC CAGTTGGCTT GTATGGACCC AGCGGATGAT 
GGACAATTCC AGGAACAACA ATCTCTGGTG AATGCACAAG AGCCAGAGGT TAGTGAATTA 
AAAAGTGTGC AGTCCTCTAA CCATGGCATC TACCTTCCTC CGGACACCCA GGAGCATGCG 
GGATCTGGGA GGGCATCCTC TATGCCACGT CTGACTATGG ATCCCCAGGT GGTAACAGAC 
CCTAGCTCTA TGCGACGTTC ATTTTCTACA ATTCGGGATA AGCGTTCAAA TTCCTCCTGG 
TTGGAGGAGT TCTCCATGGA ACGCAGCAGT GAAAACACTT ACAAGTCGCG GCGCAGAAGT 
TACCATTCCT CCCTGAGGCT GTCAGCACAC CGCCTGAATT CTGACTCTGG TCACAAGTCT 
GACACTCACC GATCAGGAGG CAGGGAGAGA GGCCGATCCA AAGAACGAAA GCACCTCCTC 
TCTCCAGATG TCTCCCGCTG CAATTCTGAA GAGCGAGGGA CCCAGGCCGA CTGGGAGTCC 
CCAGAACGTC GCCAGTCCAG GTCACCCAGT GAAGGAAGGT CACAGACCCC CAATAGACAG 
GGTACCGGTT CCCTAAGTGA GAGCTCCATC CCCTCTATTT CCGACACCAG CACCCCAAGA 
CGAAGCCGTC GCCAGCTCCC ACCTGTGCCA CCAAAGCCTC GTCCCCTCCT CTCCTACAGC 
TCCCTGATGA GACACACTGG CGGCATCTCT CCACCTCCTG ATGGAAGTGA GGGTGGATCC 
CCACTGGCCT CTCAAGCTCT GGAGAGCAAC AGCGCTTGCC TGACCGAGTC TTCCAACTCC 
TTGCACCCCC AGCAGGGCCA GCATCCTTCC CCACAGCACT ACATCTCCGA GCCCTATCTG 
GCCCTCCATG AAGACTCCCA CGCCTCAGAC TGTGGCGAGG AGGAGACACT CACCTTTGAG 
GCAGCCGTAG CCACTAGCTT GGGCCGGTCC AATACCATTG GCTCTGCGCC ACCCCTACGG 
CACAGCTGGC AGATGCCTAA TGGGCACTAT CGGCGGCGGA GGCGTGGGGG GCCTGGGCCT 
GGCATGATGT GTGGAGCTGT CAGTGACCTC CTGAGTGACA CGGAAGAAGA CGATAAGTGC 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011246218.1
CDS	11493..18455
Translation	MARFGEAVVVGRPGSGDGDSDQSRNRQGTPVPASGPAAAYKQSKAQRARTMALYNPIPVRQNCFTVNRSLFIFGEDNIVRKYAKKLIDWPPFEYMILATIIANCIVLALEQHLPEDDKTPMSRRLEKTEPYFIGIFCFEAGIKIVALGFIFHKGSYLRNGWNVMDFIVVLSGILATAGTHFNTHVDLRTLRAVRVLRPLKLVSGIPSLQIVLKSIMKAMVPLLQIGLLLFFAILMFAIIGLEFYSGKLHRACFMNNSGILEGFDPPHPCGVQGCPAGYECKDWIGPNDGITQFDNILFAVLTVFQCITMEGWTTVLYNTNDALGATWNWLYFIPLIIIGSFFVLNLVLGVLSGEFAKERERVENRRAFMKLRRQQQIERELNGYRAWIDKAEEVMLAEENKNSGTSALEVLRRATIKRSRTEAMTRDSSDEHCVDISSVGTPLARASIKSTKVDGASYFRHKERLLRISIRHMVKSQVFYWIVLSVVALNTACVAIVHHNQPQWLTHLLYYAEFLFLGLFLLEMSLKMYGMGPRLYFHSSFNCFDFGVTVGSIFEVVWAIFRPGTSFGISVLRALRLLRIFKITKYWASLRNLVVSLMSSMKSIISLLFLLFLFIVVFALLGMQLFGGRFNFNDGTPSANFDTFPAAIMTVFQILTGEDWNEVMYNGIRSQGGVSSGMWSAIYFIVLTLFGNYTLLNVFLAIAVDNLANAQELTKDEQEEEEAFNQKHALQKAKEVSPMSAPNMPSIERDRRRRHHMSMWEPRSSHLRERRRRHHMSVWEQRTSQLRRHMQMSSQEALNKEEAPPMNPLNPLNPLSPLNPLNAHPSLYRRPRPIEGLALGLGLEKCEEERISRGGSLKGDIGGLTSALDNQRSPLSLGKREPPWLPRSCHGNCDPIQQEAGGGETVVTFEDRARHRQSQRRSRHRRVRTEGKDSASASRSRSASQERSLDEGVSVEGEKEHEPHSSHRSKEPTIHEEERTQDLRRTNSLMVPRGSGLVGALDEAETPLVQPQPELEVGKDAALTEQEAEGSSEQALLGDVQLDVGRGISQSEPDLSCMTANMDKATTESTSVTVAIPDVDPLVDSTVVNISNKTDGEASPLKEAETKEEEEEVEKKKKQKKEKRETGKAMVPHSSMFIFSTTNPIRRACHYIVNLRYFEMCILLVIAASSIALAAEDPVLTNSERNKVLRYFDYVFTGVFTFEMVIKMIDQGLILQDGSYFRDLWNILDFVVVVGALVAFALANALGTNKGRDIKTIKSLRVLRVLRPLKTIKRLPKLKAVFDCVVTSLKNVFNILIVYKLFMFIFAVIAVQLFKGKFFYCTDSSKDTEKECIGNYVDHEKNKMEVKGREWKRHEFHYDNIIWALLTLFTVSTGEGWPQVLQHSVDVTEEDRGPSRSNRMEMSIFYVVYFVVFPFFFVNIFVALIIITFQEQGDKMMEECSLEKNERACIDFAISAKPLTRYMPQNRHTFQYRVWHFVVSPSFEYTIMAMIALNTVVLMMKYYSAPCTYELALKYLNIAFTMVFSLECVLKVIAFGFLNYFRDTWNIFDFITVIGSITEIILTDSKLVNTSGFNMSFLKLFRAARLIKLLRQGYTIRILLWTFVQSFKALPYVCLLIAMLFFIYAIIGMQVFGNIKLDEESHINRHNNFRSFFGSLMLLFRSATGEAWQEIMLSCLGEKGCEPDTTAPSGQNESERCGTDLAYVYFVSFIFFCSFLMLNLFVAVIMDNFEYLTRDSSILGPHHLDEFVRVWAEYDRAACGRIHYTEMYEMLTLMSPPLGLGKRCPSKVAYKRLVLMNMPVAEDMTVHFTSTLMALIRTALDIKIAKGGADRQQLDSELQKETLAIWPHLSQKMLDLLVPMPKASDLTVGKIYAAMMIMDYYKQSKVKKQRQQLEEQKNAPMFQRMEPSSLPQEIIANAKALPYLQQDPVSGLSGRSGYPSMSPLSPQEIFQLACMDPADDGQFQEQQSLVNAQEPEVSELKSVQSSNHGIYLPPDTQEHAGSGRASSMPRLTMDPQVVTDPSSMRRSFSTIRDKRSNSSWLEEFSMERSSENTYKSRRRSYHSSLRLSAHRLNSDSGHKSDTHRSGGRERGRSKERKHLLSPDVSRCNSEERGTQADWESPERRQSRSPSEGRSQTPNRQGTGSLSESSIPSISDTSTPRRSRRQLPPVPPKPRPLLSYSSLMRHTGGISPPPDGSEGGSPLASQALESNSACLTESSNSLHPQQGQHPSPQHYISEPYLALHEDSHASDCGEEETLTFEAAVATSLGRSNTIGSAPPLRHSWQMPNGHYRRRRRGGPGPGMMCGAVSDLLSDTEEDDKC

Target ORF information:

RefSeq Version	XM_011247916.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus calcium channel, voltage-dependent, R type, alpha 1E subunit (Cacna1e), transcript variant X16, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011247916.2

ORF Insert Sequence:

ATGGCTCGCT TCGGGGAGGC GGTGGTCGTT GGCAGGCCAG GCTCAGGCGA TGGAGACTCG 
GACCAGAGCA GGAACCGACA AGGAACCCCC GTCCCGGCCT CGGGGCCGGC GGCCGCCTAC 
AAGCAGTCAA AAGCGCAGAG GGCGCGGACT ATGGCTTTGT ACAACCCCAT ACCAGTCCGG 
CAGAACTGTT TCACGGTCAA CAGATCCCTG TTCATCTTCG GAGAAGATAA CATTGTCAGG 
AAATACGCCA AGAAGCTCAT CGATTGGCCG CCATTTGAGT ACATGATCCT GGCCACCATC 
ATTGCCAACT GCATCGTCTT GGCCCTGGAG CAGCATCTTC CTGAGGATGA CAAGACCCCA 
ATGTCTCGAA GACTGGAGAA GACAGAACCA TATTTCATTG GGATCTTCTG CTTTGAAGCT 
GGGATCAAAA TTGTGGCTCT AGGGTTCATC TTCCATAAGG GTTCATACCT CCGTAATGGC 
TGGAATGTCA TGGACTTCAT CGTGGTCCTT AGTGGCATCC TGGCCACTGC GGGAACCCAC 
TTCAACACCC ACGTGGACCT GCGGACCCTC CGGGCTGTGC GTGTCCTGAG GCCTTTAAAG 
CTGGTATCAG GAATACCTAG CCTGCAGATC GTGCTGAAGT CTATCATGAA GGCCATGGTG 
CCTCTTCTGC AGATTGGCCT TCTGCTCTTC TTTGCCATCC TCATGTTTGC TATCATTGGC 
TTGGAGTTCT ACAGTGGCAA GTTGCATCGA GCATGCTTCA TGAACAATTC AGGTATTCTA 
GAGGGGTTTG ATCCTCCTCA CCCGTGTGGT GTGCAAGGCT GCCCGGCTGG TTATGAATGT 
AAGGACTGGA TCGGCCCCAA TGACGGGATC ACCCAGTTTG ACAACATCCT TTTTGCCGTG 
CTGACTGTCT TCCAATGCAT CACTATGGAA GGGTGGACCA CTGTGCTGTA CAATACCAAT 
GATGCCTTAG GAGCCACCTG GAACTGGCTG TACTTCATCC CTCTCATCAT CATCGGATCC 
TTCTTTGTTC TCAACCTCGT CCTGGGAGTG CTTTCCGGGG AATTTGCCAA AGAAAGAGAG 
AGAGTGGAGA ACCGAAGAGC GTTCATGAAG CTCCGGCGCC AGCAACAGAT TGAGCGAGAA 
TTGAACGGCT ACCGTGCCTG GATAGACAAA GCAGAGGAAG TCATGCTTGC TGAAGAAAAT 
AAAAACTCGG GAACATCAGC CTTGGAAGTG CTTCGAAGGG CAACCATCAA AAGGAGCCGG 
ACGGAGGCCA TGACCCGAGA CTCCAGCGAT GAGCACTGCG TTGATATCTC TTCAGTGGGC 
ACACCTCTTG CCAGAGCCAG TATCAAGAGC ACAAAAGTAG ATGGGGCCTC CTATTTCCGG 
CACAAGGAAC GGCTTCTGCG CATCTCTATC CGCCACATGG TCAAGTCCCA AGTCTTTTAC 
TGGATCGTCC TGAGTGTTGT GGCTCTTAAT ACTGCCTGTG TGGCCATTGT TCATCACAAC 
CAGCCACAGT GGCTCACTCA CCTCCTCTAC TATGCAGAGT TTTTGTTTCT GGGACTCTTC 
CTGTTGGAGA TGTCCCTGAA GATGTATGGC ATGGGGCCAC GCCTCTATTT TCACTCATCC 
TTCAACTGCT TTGATTTTGG GGTCACAGTG GGCAGCATCT TTGAGGTTGT CTGGGCAATC 
TTCAGACCTG GCACATCTTT TGGAATCAGT GTCTTACGAG CTCTCCGGCT CCTAAGGATA 
TTTAAAATAA CCAAGTATTG GGCATCCCTC CGGAATCTGG TTGTCTCCTT GATGAGCTCC 
ATGAAGTCTA TCATCAGCTT ACTCTTCCTT CTCTTCCTCT TCATCGTTGT CTTTGCTCTC 
CTGGGAATGC AGCTCTTTGG AGGCAGGTTT AACTTTAATG ATGGGACTCC TTCAGCTAAT 
TTTGACACCT TCCCTGCAGC TATCATGACA GTGTTCCAGA TCCTGACAGG GGAAGACTGG 
AATGAGGTGA TGTACAATGG CATTCGCTCC CAGGGTGGGG TCAGCTCAGG CATGTGGTCT 
GCCATCTACT TCATTGTGCT CACCTTGTTT GGAAACTACA CGCTGCTAAA TGTATTCTTG 
GCCATCGCTG TGGACAATCT GGCCAATGCC CAGGAACTGA CCAAGGATGA GCAGGAGGAA 
GAAGAAGCCT TCAACCAGAA ACATGCACTG CAGAAGGCCA AGGAGGTCAG CCCGATGTCT 
GCTCCCAACA TGCCTTCCAT TGAAAGAGAC AGAAGGAGAA GACACCACAT GTCGATGTGG 
GAGCCACGCA GCAGCCACCT GAGGGAGCGG AGGCGCCGGC ACCACATGTC TGTGTGGGAA 
CAACGCACCA GCCAGCTGAG GAGGCACATG CAAATGTCTA GCCAAGAAGC CCTCAACAAA 
GAGGAGGCCC CGCCAATGAA CCCCCTCAAT CCACTCAACC CACTGAGTCC TCTCAACCCC 
CTCAATGCTC ACCCCAGCCT TTATCGGAGG CCCAGGCCCA TTGAGGGCCT AGCCCTAGGT 
CTGGGTCTTG AAAAGTGTGA AGAAGAACGC ATCAGTCGTG GGGGCTCCCT CAAGGGGGAC 
ATAGGGGGCC TGACTAGCGC CCTGGACAAC CAGAGGAGCC CCTTGTCCTT GGGCAAACGG 
GAGCCACCGT GGCTGCCTAG ATCCTGTCAT GGAAACTGTG ACCCAATCCA GCAGGAGGCT 
GGGGGAGGAG AGACTGTGGT GACTTTTGAG GACCGGGCCA GGCACAGGCA GAGTCAGAGG 
CGAAGCCGGC ATCGCCGAGT CAGGACTGAG GGCAAGGACT CTGCCTCTGC ATCCCGAAGC 
AGGTCTGCCA GCCAGGAGCG GAGTCTGGAT GAAGGGGTGT CCGTTGAGGG GGAGAAGGAA 
CATGAGCCTC ACAGCAGCCA CAGGAGCAAA GAGCCGACCA TCCACGAAGA GGAGAGAACC 
CAGGACTTAA GGAGGACCAA CAGTCTGATG GTCCCCAGGG GCTCTGGACT GGTAGGGGCT 
CTTGATGAAG CAGAAACACC TCTAGTCCAG CCCCAACCTG AGCTGGAAGT GGGGAAGGAT 
GCAGCTCTGA CAGAGCAGGA GGCTGAAGGC AGCAGTGAAC AAGCCCTTCT TGGGGACGTA 
CAGCTGGATG TGGGCCGGGG CATTAGCCAG AGTGAACCGG ATCTCTCCTG CATGACAGCC 
AACATGGACA AGGCTACCAC GGAGAGCACC AGTGTCACGG TCGCCATCCC TGATGTGGAC 
CCCTTGGTGG ACTCGACAGT GGTGAACATT AGCAACAAGA CGGATGGAGA AGCCAGTCCC 
TTGAAGGAGG CAGAGACTAA AGAGGAAGAA GAAGAGGTAG AGAAGAAGAA GAAGCAGAAG 
AAGGAGAAGC GAGAGACTGG AAAAGCCATG GTGCCCCATA GCTCTATGTT CATCTTCAGC 
ACCACTAACC CGATCCGCAG GGCCTGCCAC TACATTGTGA ACCTACGCTA CTTTGAAATG 
TGCATTCTCT TGGTGATTGC GGCCAGCAGC ATTGCCCTGG CAGCCGAGGA CCCTGTACTA 
ACTAACTCGG AGCGTAACAA AGTCCTGAGA TATTTCGACT ATGTTTTCAC AGGAGTGTTT 
ACCTTTGAGA TGGTTATAAA GATGATAGAC CAAGGCTTGA TCCTGCAGGA TGGATCATAC 
TTCAGAGACC TCTGGAACAT CCTGGACTTT GTAGTAGTGG TTGGTGCATT GGTAGCATTT 
GCTCTGGCGA ACGCTTTGGG AACCAACAAG GGGAGGGATA TCAAGACTAT CAAGTCTCTG 
AGGGTACTTC GAGTTCTGCG GCCACTGAAA ACCATCAAGC GCTTGCCCAA GCTGAAGGCT 
GTGTTTGACT GCGTGGTGAC CTCCTTGAAG AATGTCTTCA ACATACTCAT TGTCTACAAG 
CTCTTCATGT TCATCTTTGC CGTCATCGCT GTTCAGCTAT TCAAAGGAAA GTTCTTTTAT 
TGCACTGACA GTTCCAAGGA CACAGAGAAG GAGTGCATAG GCAACTATGT AGACCACGAG 
AAAAACAAGA TGGAGGTGAA GGGCCGGGAA TGGAAGCGTC ATGAATTCCA CTATGACAAC 
ATCATCTGGG CCCTGCTTAC CCTCTTCACA GTCTCTACAG GGGAAGGATG GCCTCAGGTT 
CTGCAGCACT CTGTAGATGT GACAGAGGAG GACAGAGGCC CAAGTCGCAG CAACCGCATG 
GAGATGTCCA TCTTTTATGT GGTCTACTTT GTGGTCTTCC CTTTCTTCTT TGTCAATATC 
TTCGTGGCTC TCATTATCAT CACCTTCCAG GAGCAAGGGG ACAAGATGAT GGAGGAGTGT 
AGCCTGGAGA AGAATGAGCG GGCATGCATC GACTTCGCCA TTAGTGCCAA ACCTCTCACC 
CGCTACATGC CACAGAACAG GCACACCTTC CAGTACCGTG TGTGGCACTT TGTAGTCTCC 
CCATCCTTTG AGTATACCAT CATGGCCATG ATTGCCTTGA ACACTGTCGT GCTGATGATG 
AAGTACTACT CTGCTCCTTG CACCTATGAG CTCGCCTTAA AGTACCTGAA CATCGCCTTC 
ACTATGGTGT TTTCCCTGGA GTGTGTCCTA AAGGTCATTG CTTTCGGCTT CCTGAACTAT 
TTCCGAGATA CCTGGAACAT CTTTGACTTC ATCACGGTGA TCGGCAGTAT CACAGAAATT 
ATCCTGACAG ATAGCAAGCT GGTGAACACC AGTGGCTTCA ATATGAGCTT TCTGAAGCTC 
TTCCGAGCTG CACGGCTCAT AAAGCTTCTG AGACAGGGCT ACACCATCCG CATCTTACTG 
TGGACCTTCG TGCAGTCCTT TAAGGCTCTT CCCTACGTCT GCCTTCTCAT TGCCATGCTT 
TTCTTCATCT ACGCCATCAT TGGGATGCAG GTGTTTGGAA ACATAAAGTT AGATGAGGAG 
AGTCACATCA ACCGGCACAA CAACTTCCGG AGTTTCTTTG GATCCCTCAT GCTGCTTTTC 
AGGAGTGCCA CGGGGGAGGC TTGGCAGGAG ATTATGCTGT CCTGTCTCGG TGAGAAGGGT 
TGTGAGCCTG ACACCACTGC ACCCTCAGGG CAGAATGAGA GTGAGCGCTG TGGCACTGAC 
CTGGCCTACG TTTATTTTGT CTCCTTCATC TTCTTCTGCT CCTTCCTGAT GCTCAACCTG 
TTCGTGGCTG TCATCATGGA CAACTTTGAG TACCTGACTC GAGATTCCTC CATCCTGGGG 
CCTCACCACT TAGATGAGTT TGTCCGTGTC TGGGCAGAAT ATGACAGAGC AGCATGTGGC 
CGCATCCATT ACACTGAGAT GTATGAAATG CTGACTCTCA TGTCACCACC GCTAGGCCTC 
GGCAAGAGAT GTCCCTCCAA AGTGGCCTAT AAGAGGCTGG TCCTGATGAA CATGCCAGTG 
GCTGAGGACA TGACAGTCCA CTTCACCTCC ACACTTATGG CTCTGATCCG GACAGCTCTG 
GACATTAAAA TCGCCAAAGG TGGTGCAGAC AGACAGCAGC TAGACTCGGA GCTGCAAAAA 
GAGACCCTGG CCATTTGGCC TCACCTGTCC CAAAAGATGT TGGATCTGCT TGTGCCCATG 
CCCAAAGCCT CTGACCTGAC TGTGGGCAAG ATCTATGCAG CAATGATGAT CATGGACTAC 
TATAAACAGA GTAAGGTAAA GAAACAGAGA CAGCAGCTGG AAGAACAGAA AAATGCACCC 
ATGTTCCAGC GCATGGAGCC CTCATCACTG CCTCAGGAGA TCATTGCTAA TGCCAAAGCC 
CTGCCTTATC TCCAGCAGGA CCCTGTTTCT GGCCTGAGTG GTCGGAGTGG ATACCCTTCA 
ATGAGTCCAC TCTCCCCTCA AGAAATATTC CAGTTGGCTT GTATGGACCC AGCGGATGAT 
GGACAATTCC AGGAACAACA ATCTCTGGTG AATGCACAAG AGCCAGAGGT TAGTGAATTA 
AAAAGTGTGC AGTCCTCTAA CCATGGCATC TACCTTCCTC CGGACACCCA GGAGCATGCG 
GGATCTGGGA GGGCATCCTC TATGCCACGT CTGACTATGG ATCCCCAGGT GGTAACAGAC 
CCTAGCTCTA TGCGACGTTC ATTTTCTACA ATTCGGGATA AGCGTTCAAA TTCCTCCTGG 
TTGGAGGAGT TCTCCATGGA ACGCAGCAGT GAAAACACTT ACAAGTCGCG GCGCAGAAGT 
TACCATTCCT CCCTGAGGCT GTCAGCACAC CGCCTGAATT CTGACTCTGG TCACAAGTCT 
GACACTCACC GATCAGGAGG CAGGGAGAGA GGCCGATCCA AAGAACGAAA GCACCTCCTC 
TCTCCAGATG TCTCCCGCTG CAATTCTGAA GAGCGAGGGA CCCAGGCCGA CTGGGAGTCC 
CCAGAACGTC GCCAGTCCAG GTCACCCAGT GAAGGAAGGT CACAGACCCC CAATAGACAG 
GGTACCGGTT CCCTAAGTGA GAGCTCCATC CCCTCTATTT CCGACACCAG CACCCCAAGA 
CGAAGCCGTC GCCAGCTCCC ACCTGTGCCA CCAAAGCCTC GTCCCCTCCT CTCCTACAGC 
TCCCTGATGA GACACACTGG CGGCATCTCT CCACCTCCTG ATGGAAGTGA GGGTGGATCC 
CCACTGGCCT CTCAAGCTCT GGAGAGCAAC AGCGCTTGCC TGACCGAGTC TTCCAACTCC 
TTGCACCCCC AGCAGGGCCA GCATCCTTCC CCACAGCACT ACATCTCCGA GCCCTATCTG 
GCCCTCCATG AAGACTCCCA CGCCTCAGAC TGTGGCGAGG AGGAGACACT CACCTTTGAG 
GCAGCCGTAG CCACTAGCTT GGGCCGGTCC AATACCATTG GCTCTGCGCC ACCCCTACGG 
CACAGCTGGC AGATGCCTAA TGGGCACTAT CGGCGGCGGA GGCGTGGGGG GCCTGGGCCT 
GGCATGATGT GTGGAGCTGT CAGTGACCTC CTGAGTGACA CGGAAGAAGA CGATAAGTGC 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cacna1e ( XM_017312848.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017168337.1 Mus musculus calcium channel, voltage-dependent, R type, alpha 1E subunit (Cacna1e), transcript variant X18, mRNA.

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CloneID	OMu67267
Clone ID Related Accession (Same CDS sequence)	XM_011247916.3 , XM_011247916.2 , XM_017312848.1
Accession Version	XM_017312848.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6963bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	voltage-dependent R-type calcium channel subunit alpha-1E isoform X14
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTCGCT TCGGGGAGGC GGTGGTCGTT GGCAGGCCAG GCTCAGGCGA TGGAGACTCG 
GACCAGAGCA GGAACCGACA AGGAACCCCC GTCCCGGCCT CGGGGCCGGC GGCCGCCTAC 
AAGCAGTCAA AAGCGCAGAG GGCGCGGACT ATGGCTTTGT ACAACCCCAT ACCAGTCCGG 
CAGAACTGTT TCACGGTCAA CAGATCCCTG TTCATCTTCG GAGAAGATAA CATTGTCAGG 
AAATACGCCA AGAAGCTCAT CGATTGGCCG CCATTTGAGT ACATGATCCT GGCCACCATC 
ATTGCCAACT GCATCGTCTT GGCCCTGGAG CAGCATCTTC CTGAGGATGA CAAGACCCCA 
ATGTCTCGAA GACTGGAGAA GACAGAACCA TATTTCATTG GGATCTTCTG CTTTGAAGCT 
GGGATCAAAA TTGTGGCTCT AGGGTTCATC TTCCATAAGG GTTCATACCT CCGTAATGGC 
TGGAATGTCA TGGACTTCAT CGTGGTCCTT AGTGGCATCC TGGCCACTGC GGGAACCCAC 
TTCAACACCC ACGTGGACCT GCGGACCCTC CGGGCTGTGC GTGTCCTGAG GCCTTTAAAG 
CTGGTATCAG GAATACCTAG CCTGCAGATC GTGCTGAAGT CTATCATGAA GGCCATGGTG 
CCTCTTCTGC AGATTGGCCT TCTGCTCTTC TTTGCCATCC TCATGTTTGC TATCATTGGC 
TTGGAGTTCT ACAGTGGCAA GTTGCATCGA GCATGCTTCA TGAACAATTC AGGTATTCTA 
GAGGGGTTTG ATCCTCCTCA CCCGTGTGGT GTGCAAGGCT GCCCGGCTGG TTATGAATGT 
AAGGACTGGA TCGGCCCCAA TGACGGGATC ACCCAGTTTG ACAACATCCT TTTTGCCGTG 
CTGACTGTCT TCCAATGCAT CACTATGGAA GGGTGGACCA CTGTGCTGTA CAATACCAAT 
GATGCCTTAG GAGCCACCTG GAACTGGCTG TACTTCATCC CTCTCATCAT CATCGGATCC 
TTCTTTGTTC TCAACCTCGT CCTGGGAGTG CTTTCCGGGG AATTTGCCAA AGAAAGAGAG 
AGAGTGGAGA ACCGAAGAGC GTTCATGAAG CTCCGGCGCC AGCAACAGAT TGAGCGAGAA 
TTGAACGGCT ACCGTGCCTG GATAGACAAA GCAGAGGAAG TCATGCTTGC TGAAGAAAAT 
AAAAACTCGG GAACATCAGC CTTGGAAGTG CTTCGAAGGG CAACCATCAA AAGGAGCCGG 
ACGGAGGCCA TGACCCGAGA CTCCAGCGAT GAGCACTGCG TTGATATCTC TTCAGTGGGC 
ACACCTCTTG CCAGAGCCAG TATCAAGAGC ACAAAAGTAG ATGGGGCCTC CTATTTCCGG 
CACAAGGAAC GGCTTCTGCG CATCTCTATC CGCCACATGG TCAAGTCCCA AGTCTTTTAC 
TGGATCGTCC TGAGTGTTGT GGCTCTTAAT ACTGCCTGTG TGGCCATTGT TCATCACAAC 
CAGCCACAGT GGCTCACTCA CCTCCTCTAC TATGCAGAGT TTTTGTTTCT GGGACTCTTC 
CTGTTGGAGA TGTCCCTGAA GATGTATGGC ATGGGGCCAC GCCTCTATTT TCACTCATCC 
TTCAACTGCT TTGATTTTGG GGTCACAGTG GGCAGCATCT TTGAGGTTGT CTGGGCAATC 
TTCAGACCTG GCACATCTTT TGGAATCAGT GTCTTACGAG CTCTCCGGCT CCTAAGGATA 
TTTAAAATAA CCAAGTATTG GGCATCCCTC CGGAATCTGG TTGTCTCCTT GATGAGCTCC 
ATGAAGTCTA TCATCAGCTT ACTCTTCCTT CTCTTCCTCT TCATCGTTGT CTTTGCTCTC 
CTGGGAATGC AGCTCTTTGG AGGCAGGTTT AACTTTAATG ATGGGACTCC TTCAGCTAAT 
TTTGACACCT TCCCTGCAGC TATCATGACA GTGTTCCAGA TCCTGACAGG GGAAGACTGG 
AATGAGGTGA TGTACAATGG CATTCGCTCC CAGGGTGGGG TCAGCTCAGG CATGTGGTCT 
GCCATCTACT TCATTGTGCT CACCTTGTTT GGAAACTACA CGCTGCTAAA TGTATTCTTG 
GCCATCGCTG TGGACAATCT GGCCAATGCC CAGGAACTGA CCAAGGATGA GCAGGAGGAA 
GAAGAAGCCT TCAACCAGAA ACATGCACTG CAGAAGGCCA AGGAGGTCAG CCCGATGTCT 
GCTCCCAACA TGCCTTCCAT TGAAAGAGAC AGAAGGAGAA GACACCACAT GTCGATGTGG 
GAGCCACGCA GCAGCCACCT GAGGGAGCGG AGGCGCCGGC ACCACATGTC TGTGTGGGAA 
CAACGCACCA GCCAGCTGAG GAGGCACATG CAAATGTCTA GCCAAGAAGC CCTCAACAAA 
GAGGAGGCCC CGCCAATGAA CCCCCTCAAT CCACTCAACC CACTGAGTCC TCTCAACCCC 
CTCAATGCTC ACCCCAGCCT TTATCGGAGG CCCAGGCCCA TTGAGGGCCT AGCCCTAGGT 
CTGGGTCTTG AAAAGTGTGA AGAAGAACGC ATCAGTCGTG GGGGCTCCCT CAAGGGGGAC 
ATAGGGGGCC TGACTAGCGC CCTGGACAAC CAGAGGAGCC CCTTGTCCTT GGGCAAACGG 
GAGCCACCGT GGCTGCCTAG ATCCTGTCAT GGAAACTGTG ACCCAATCCA GCAGGAGGCT 
GGGGGAGGAG AGACTGTGGT GACTTTTGAG GACCGGGCCA GGCACAGGCA GAGTCAGAGG 
CGAAGCCGGC ATCGCCGAGT CAGGACTGAG GGCAAGGACT CTGCCTCTGC ATCCCGAAGC 
AGGTCTGCCA GCCAGGAGCG GAGTCTGGAT GAAGGGGTGT CCGTTGAGGG GGAGAAGGAA 
CATGAGCCTC ACAGCAGCCA CAGGAGCAAA GAGCCGACCA TCCACGAAGA GGAGAGAACC 
CAGGACTTAA GGAGGACCAA CAGTCTGATG GTCCCCAGGG GCTCTGGACT GGTAGGGGCT 
CTTGATGAAG CAGAAACACC TCTAGTCCAG CCCCAACCTG AGCTGGAAGT GGGGAAGGAT 
GCAGCTCTGA CAGAGCAGGA GGCTGAAGGC AGCAGTGAAC AAGCCCTTCT TGGGGACGTA 
CAGCTGGATG TGGGCCGGGG CATTAGCCAG AGTGAACCGG ATCTCTCCTG CATGACAGCC 
AACATGGACA AGGCTACCAC GGAGAGCACC AGTGTCACGG TCGCCATCCC TGATGTGGAC 
CCCTTGGTGG ACTCGACAGT GGTGAACATT AGCAACAAGA CGGATGGAGA AGCCAGTCCC 
TTGAAGGAGG CAGAGACTAA AGAGGAAGAA GAAGAGGTAG AGAAGAAGAA GAAGCAGAAG 
AAGGAGAAGC GAGAGACTGG AAAAGCCATG GTGCCCCATA GCTCTATGTT CATCTTCAGC 
ACCACTAACC CGATCCGCAG GGCCTGCCAC TACATTGTGA ACCTACGCTA CTTTGAAATG 
TGCATTCTCT TGGTGATTGC GGCCAGCAGC ATTGCCCTGG CAGCCGAGGA CCCTGTACTA 
ACTAACTCGG AGCGTAACAA AGTCCTGAGA TATTTCGACT ATGTTTTCAC AGGAGTGTTT 
ACCTTTGAGA TGGTTATAAA GATGATAGAC CAAGGCTTGA TCCTGCAGGA TGGATCATAC 
TTCAGAGACC TCTGGAACAT CCTGGACTTT GTAGTAGTGG TTGGTGCATT GGTAGCATTT 
GCTCTGGCGA ACGCTTTGGG AACCAACAAG GGGAGGGATA TCAAGACTAT CAAGTCTCTG 
AGGGTACTTC GAGTTCTGCG GCCACTGAAA ACCATCAAGC GCTTGCCCAA GCTGAAGGCT 
GTGTTTGACT GCGTGGTGAC CTCCTTGAAG AATGTCTTCA ACATACTCAT TGTCTACAAG 
CTCTTCATGT TCATCTTTGC CGTCATCGCT GTTCAGCTAT TCAAAGGAAA GTTCTTTTAT 
TGCACTGACA GTTCCAAGGA CACAGAGAAG GAGTGCATAG GCAACTATGT AGACCACGAG 
AAAAACAAGA TGGAGGTGAA GGGCCGGGAA TGGAAGCGTC ATGAATTCCA CTATGACAAC 
ATCATCTGGG CCCTGCTTAC CCTCTTCACA GTCTCTACAG GGGAAGGATG GCCTCAGGTT 
CTGCAGCACT CTGTAGATGT GACAGAGGAG GACAGAGGCC CAAGTCGCAG CAACCGCATG 
GAGATGTCCA TCTTTTATGT GGTCTACTTT GTGGTCTTCC CTTTCTTCTT TGTCAATATC 
TTCGTGGCTC TCATTATCAT CACCTTCCAG GAGCAAGGGG ACAAGATGAT GGAGGAGTGT 
AGCCTGGAGA AGAATGAGCG GGCATGCATC GACTTCGCCA TTAGTGCCAA ACCTCTCACC 
CGCTACATGC CACAGAACAG GCACACCTTC CAGTACCGTG TGTGGCACTT TGTAGTCTCC 
CCATCCTTTG AGTATACCAT CATGGCCATG ATTGCCTTGA ACACTGTCGT GCTGATGATG 
AAGTACTACT CTGCTCCTTG CACCTATGAG CTCGCCTTAA AGTACCTGAA CATCGCCTTC 
ACTATGGTGT TTTCCCTGGA GTGTGTCCTA AAGGTCATTG CTTTCGGCTT CCTGAACTAT 
TTCCGAGATA CCTGGAACAT CTTTGACTTC ATCACGGTGA TCGGCAGTAT CACAGAAATT 
ATCCTGACAG ATAGCAAGCT GGTGAACACC AGTGGCTTCA ATATGAGCTT TCTGAAGCTC 
TTCCGAGCTG CACGGCTCAT AAAGCTTCTG AGACAGGGCT ACACCATCCG CATCTTACTG 
TGGACCTTCG TGCAGTCCTT TAAGGCTCTT CCCTACGTCT GCCTTCTCAT TGCCATGCTT 
TTCTTCATCT ACGCCATCAT TGGGATGCAG GTGTTTGGAA ACATAAAGTT AGATGAGGAG 
AGTCACATCA ACCGGCACAA CAACTTCCGG AGTTTCTTTG GATCCCTCAT GCTGCTTTTC 
AGGAGTGCCA CGGGGGAGGC TTGGCAGGAG ATTATGCTGT CCTGTCTCGG TGAGAAGGGT 
TGTGAGCCTG ACACCACTGC ACCCTCAGGG CAGAATGAGA GTGAGCGCTG TGGCACTGAC 
CTGGCCTACG TTTATTTTGT CTCCTTCATC TTCTTCTGCT CCTTCCTGAT GCTCAACCTG 
TTCGTGGCTG TCATCATGGA CAACTTTGAG TACCTGACTC GAGATTCCTC CATCCTGGGG 
CCTCACCACT TAGATGAGTT TGTCCGTGTC TGGGCAGAAT ATGACAGAGC AGCATGTGGC 
CGCATCCATT ACACTGAGAT GTATGAAATG CTGACTCTCA TGTCACCACC GCTAGGCCTC 
GGCAAGAGAT GTCCCTCCAA AGTGGCCTAT AAGAGGCTGG TCCTGATGAA CATGCCAGTG 
GCTGAGGACA TGACAGTCCA CTTCACCTCC ACACTTATGG CTCTGATCCG GACAGCTCTG 
GACATTAAAA TCGCCAAAGG TGGTGCAGAC AGACAGCAGC TAGACTCGGA GCTGCAAAAA 
GAGACCCTGG CCATTTGGCC TCACCTGTCC CAAAAGATGT TGGATCTGCT TGTGCCCATG 
CCCAAAGCCT CTGACCTGAC TGTGGGCAAG ATCTATGCAG CAATGATGAT CATGGACTAC 
TATAAACAGA GTAAGGTAAA GAAACAGAGA CAGCAGCTGG AAGAACAGAA AAATGCACCC 
ATGTTCCAGC GCATGGAGCC CTCATCACTG CCTCAGGAGA TCATTGCTAA TGCCAAAGCC 
CTGCCTTATC TCCAGCAGGA CCCTGTTTCT GGCCTGAGTG GTCGGAGTGG ATACCCTTCA 
ATGAGTCCAC TCTCCCCTCA AGAAATATTC CAGTTGGCTT GTATGGACCC AGCGGATGAT 
GGACAATTCC AGGAACAACA ATCTCTGGTG AATGCACAAG AGCCAGAGGT TAGTGAATTA 
AAAAGTGTGC AGTCCTCTAA CCATGGCATC TACCTTCCTC CGGACACCCA GGAGCATGCG 
GGATCTGGGA GGGCATCCTC TATGCCACGT CTGACTATGG ATCCCCAGGT GGTAACAGAC 
CCTAGCTCTA TGCGACGTTC ATTTTCTACA ATTCGGGATA AGCGTTCAAA TTCCTCCTGG 
TTGGAGGAGT TCTCCATGGA ACGCAGCAGT GAAAACACTT ACAAGTCGCG GCGCAGAAGT 
TACCATTCCT CCCTGAGGCT GTCAGCACAC CGCCTGAATT CTGACTCTGG TCACAAGTCT 
GACACTCACC GATCAGGAGG CAGGGAGAGA GGCCGATCCA AAGAACGAAA GCACCTCCTC 
TCTCCAGATG TCTCCCGCTG CAATTCTGAA GAGCGAGGGA CCCAGGCCGA CTGGGAGTCC 
CCAGAACGTC GCCAGTCCAG GTCACCCAGT GAAGGAAGGT CACAGACCCC CAATAGACAG 
GGTACCGGTT CCCTAAGTGA GAGCTCCATC CCCTCTATTT CCGACACCAG CACCCCAAGA 
CGAAGCCGTC GCCAGCTCCC ACCTGTGCCA CCAAAGCCTC GTCCCCTCCT CTCCTACAGC 
TCCCTGATGA GACACACTGG CGGCATCTCT CCACCTCCTG ATGGAAGTGA GGGTGGATCC 
CCACTGGCCT CTCAAGCTCT GGAGAGCAAC AGCGCTTGCC TGACCGAGTC TTCCAACTCC 
TTGCACCCCC AGCAGGGCCA GCATCCTTCC CCACAGCACT ACATCTCCGA GCCCTATCTG 
GCCCTCCATG AAGACTCCCA CGCCTCAGAC TGTGGCGAGG AGGAGACACT CACCTTTGAG 
GCAGCCGTAG CCACTAGCTT GGGCCGGTCC AATACCATTG GCTCTGCGCC ACCCCTACGG 
CACAGCTGGC AGATGCCTAA TGGGCACTAT CGGCGGCGGA GGCGTGGGGG GCCTGGGCCT 
GGCATGATGT GTGGAGCTGT CAGTGACCTC CTGAGTGACA CGGAAGAAGA CGATAAGTGC 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017168337.1
CDS	581..7543
Translation	MARFGEAVVVGRPGSGDGDSDQSRNRQGTPVPASGPAAAYKQSKAQRARTMALYNPIPVRQNCFTVNRSLFIFGEDNIVRKYAKKLIDWPPFEYMILATIIANCIVLALEQHLPEDDKTPMSRRLEKTEPYFIGIFCFEAGIKIVALGFIFHKGSYLRNGWNVMDFIVVLSGILATAGTHFNTHVDLRTLRAVRVLRPLKLVSGIPSLQIVLKSIMKAMVPLLQIGLLLFFAILMFAIIGLEFYSGKLHRACFMNNSGILEGFDPPHPCGVQGCPAGYECKDWIGPNDGITQFDNILFAVLTVFQCITMEGWTTVLYNTNDALGATWNWLYFIPLIIIGSFFVLNLVLGVLSGEFAKERERVENRRAFMKLRRQQQIERELNGYRAWIDKAEEVMLAEENKNSGTSALEVLRRATIKRSRTEAMTRDSSDEHCVDISSVGTPLARASIKSTKVDGASYFRHKERLLRISIRHMVKSQVFYWIVLSVVALNTACVAIVHHNQPQWLTHLLYYAEFLFLGLFLLEMSLKMYGMGPRLYFHSSFNCFDFGVTVGSIFEVVWAIFRPGTSFGISVLRALRLLRIFKITKYWASLRNLVVSLMSSMKSIISLLFLLFLFIVVFALLGMQLFGGRFNFNDGTPSANFDTFPAAIMTVFQILTGEDWNEVMYNGIRSQGGVSSGMWSAIYFIVLTLFGNYTLLNVFLAIAVDNLANAQELTKDEQEEEEAFNQKHALQKAKEVSPMSAPNMPSIERDRRRRHHMSMWEPRSSHLRERRRRHHMSVWEQRTSQLRRHMQMSSQEALNKEEAPPMNPLNPLNPLSPLNPLNAHPSLYRRPRPIEGLALGLGLEKCEEERISRGGSLKGDIGGLTSALDNQRSPLSLGKREPPWLPRSCHGNCDPIQQEAGGGETVVTFEDRARHRQSQRRSRHRRVRTEGKDSASASRSRSASQERSLDEGVSVEGEKEHEPHSSHRSKEPTIHEEERTQDLRRTNSLMVPRGSGLVGALDEAETPLVQPQPELEVGKDAALTEQEAEGSSEQALLGDVQLDVGRGISQSEPDLSCMTANMDKATTESTSVTVAIPDVDPLVDSTVVNISNKTDGEASPLKEAETKEEEEEVEKKKKQKKEKRETGKAMVPHSSMFIFSTTNPIRRACHYIVNLRYFEMCILLVIAASSIALAAEDPVLTNSERNKVLRYFDYVFTGVFTFEMVIKMIDQGLILQDGSYFRDLWNILDFVVVVGALVAFALANALGTNKGRDIKTIKSLRVLRVLRPLKTIKRLPKLKAVFDCVVTSLKNVFNILIVYKLFMFIFAVIAVQLFKGKFFYCTDSSKDTEKECIGNYVDHEKNKMEVKGREWKRHEFHYDNIIWALLTLFTVSTGEGWPQVLQHSVDVTEEDRGPSRSNRMEMSIFYVVYFVVFPFFFVNIFVALIIITFQEQGDKMMEECSLEKNERACIDFAISAKPLTRYMPQNRHTFQYRVWHFVVSPSFEYTIMAMIALNTVVLMMKYYSAPCTYELALKYLNIAFTMVFSLECVLKVIAFGFLNYFRDTWNIFDFITVIGSITEIILTDSKLVNTSGFNMSFLKLFRAARLIKLLRQGYTIRILLWTFVQSFKALPYVCLLIAMLFFIYAIIGMQVFGNIKLDEESHINRHNNFRSFFGSLMLLFRSATGEAWQEIMLSCLGEKGCEPDTTAPSGQNESERCGTDLAYVYFVSFIFFCSFLMLNLFVAVIMDNFEYLTRDSSILGPHHLDEFVRVWAEYDRAACGRIHYTEMYEMLTLMSPPLGLGKRCPSKVAYKRLVLMNMPVAEDMTVHFTSTLMALIRTALDIKIAKGGADRQQLDSELQKETLAIWPHLSQKMLDLLVPMPKASDLTVGKIYAAMMIMDYYKQSKVKKQRQQLEEQKNAPMFQRMEPSSLPQEIIANAKALPYLQQDPVSGLSGRSGYPSMSPLSPQEIFQLACMDPADDGQFQEQQSLVNAQEPEVSELKSVQSSNHGIYLPPDTQEHAGSGRASSMPRLTMDPQVVTDPSSMRRSFSTIRDKRSNSSWLEEFSMERSSENTYKSRRRSYHSSLRLSAHRLNSDSGHKSDTHRSGGRERGRSKERKHLLSPDVSRCNSEERGTQADWESPERRQSRSPSEGRSQTPNRQGTGSLSESSIPSISDTSTPRRSRRQLPPVPPKPRPLLSYSSLMRHTGGISPPPDGSEGGSPLASQALESNSACLTESSNSLHPQQGQHPSPQHYISEPYLALHEDSHASDCGEEETLTFEAAVATSLGRSNTIGSAPPLRHSWQMPNGHYRRRRRGGPGPGMMCGAVSDLLSDTEEDDKC

Target ORF information:

RefSeq Version	XM_017312848.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus calcium channel, voltage-dependent, R type, alpha 1E subunit (Cacna1e), transcript variant X18, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017312848.1

ORF Insert Sequence:

ATGGCTCGCT TCGGGGAGGC GGTGGTCGTT GGCAGGCCAG GCTCAGGCGA TGGAGACTCG 
GACCAGAGCA GGAACCGACA AGGAACCCCC GTCCCGGCCT CGGGGCCGGC GGCCGCCTAC 
AAGCAGTCAA AAGCGCAGAG GGCGCGGACT ATGGCTTTGT ACAACCCCAT ACCAGTCCGG 
CAGAACTGTT TCACGGTCAA CAGATCCCTG TTCATCTTCG GAGAAGATAA CATTGTCAGG 
AAATACGCCA AGAAGCTCAT CGATTGGCCG CCATTTGAGT ACATGATCCT GGCCACCATC 
ATTGCCAACT GCATCGTCTT GGCCCTGGAG CAGCATCTTC CTGAGGATGA CAAGACCCCA 
ATGTCTCGAA GACTGGAGAA GACAGAACCA TATTTCATTG GGATCTTCTG CTTTGAAGCT 
GGGATCAAAA TTGTGGCTCT AGGGTTCATC TTCCATAAGG GTTCATACCT CCGTAATGGC 
TGGAATGTCA TGGACTTCAT CGTGGTCCTT AGTGGCATCC TGGCCACTGC GGGAACCCAC 
TTCAACACCC ACGTGGACCT GCGGACCCTC CGGGCTGTGC GTGTCCTGAG GCCTTTAAAG 
CTGGTATCAG GAATACCTAG CCTGCAGATC GTGCTGAAGT CTATCATGAA GGCCATGGTG 
CCTCTTCTGC AGATTGGCCT TCTGCTCTTC TTTGCCATCC TCATGTTTGC TATCATTGGC 
TTGGAGTTCT ACAGTGGCAA GTTGCATCGA GCATGCTTCA TGAACAATTC AGGTATTCTA 
GAGGGGTTTG ATCCTCCTCA CCCGTGTGGT GTGCAAGGCT GCCCGGCTGG TTATGAATGT 
AAGGACTGGA TCGGCCCCAA TGACGGGATC ACCCAGTTTG ACAACATCCT TTTTGCCGTG 
CTGACTGTCT TCCAATGCAT CACTATGGAA GGGTGGACCA CTGTGCTGTA CAATACCAAT 
GATGCCTTAG GAGCCACCTG GAACTGGCTG TACTTCATCC CTCTCATCAT CATCGGATCC 
TTCTTTGTTC TCAACCTCGT CCTGGGAGTG CTTTCCGGGG AATTTGCCAA AGAAAGAGAG 
AGAGTGGAGA ACCGAAGAGC GTTCATGAAG CTCCGGCGCC AGCAACAGAT TGAGCGAGAA 
TTGAACGGCT ACCGTGCCTG GATAGACAAA GCAGAGGAAG TCATGCTTGC TGAAGAAAAT 
AAAAACTCGG GAACATCAGC CTTGGAAGTG CTTCGAAGGG CAACCATCAA AAGGAGCCGG 
ACGGAGGCCA TGACCCGAGA CTCCAGCGAT GAGCACTGCG TTGATATCTC TTCAGTGGGC 
ACACCTCTTG CCAGAGCCAG TATCAAGAGC ACAAAAGTAG ATGGGGCCTC CTATTTCCGG 
CACAAGGAAC GGCTTCTGCG CATCTCTATC CGCCACATGG TCAAGTCCCA AGTCTTTTAC 
TGGATCGTCC TGAGTGTTGT GGCTCTTAAT ACTGCCTGTG TGGCCATTGT TCATCACAAC 
CAGCCACAGT GGCTCACTCA CCTCCTCTAC TATGCAGAGT TTTTGTTTCT GGGACTCTTC 
CTGTTGGAGA TGTCCCTGAA GATGTATGGC ATGGGGCCAC GCCTCTATTT TCACTCATCC 
TTCAACTGCT TTGATTTTGG GGTCACAGTG GGCAGCATCT TTGAGGTTGT CTGGGCAATC 
TTCAGACCTG GCACATCTTT TGGAATCAGT GTCTTACGAG CTCTCCGGCT CCTAAGGATA 
TTTAAAATAA CCAAGTATTG GGCATCCCTC CGGAATCTGG TTGTCTCCTT GATGAGCTCC 
ATGAAGTCTA TCATCAGCTT ACTCTTCCTT CTCTTCCTCT TCATCGTTGT CTTTGCTCTC 
CTGGGAATGC AGCTCTTTGG AGGCAGGTTT AACTTTAATG ATGGGACTCC TTCAGCTAAT 
TTTGACACCT TCCCTGCAGC TATCATGACA GTGTTCCAGA TCCTGACAGG GGAAGACTGG 
AATGAGGTGA TGTACAATGG CATTCGCTCC CAGGGTGGGG TCAGCTCAGG CATGTGGTCT 
GCCATCTACT TCATTGTGCT CACCTTGTTT GGAAACTACA CGCTGCTAAA TGTATTCTTG 
GCCATCGCTG TGGACAATCT GGCCAATGCC CAGGAACTGA CCAAGGATGA GCAGGAGGAA 
GAAGAAGCCT TCAACCAGAA ACATGCACTG CAGAAGGCCA AGGAGGTCAG CCCGATGTCT 
GCTCCCAACA TGCCTTCCAT TGAAAGAGAC AGAAGGAGAA GACACCACAT GTCGATGTGG 
GAGCCACGCA GCAGCCACCT GAGGGAGCGG AGGCGCCGGC ACCACATGTC TGTGTGGGAA 
CAACGCACCA GCCAGCTGAG GAGGCACATG CAAATGTCTA GCCAAGAAGC CCTCAACAAA 
GAGGAGGCCC CGCCAATGAA CCCCCTCAAT CCACTCAACC CACTGAGTCC TCTCAACCCC 
CTCAATGCTC ACCCCAGCCT TTATCGGAGG CCCAGGCCCA TTGAGGGCCT AGCCCTAGGT 
CTGGGTCTTG AAAAGTGTGA AGAAGAACGC ATCAGTCGTG GGGGCTCCCT CAAGGGGGAC 
ATAGGGGGCC TGACTAGCGC CCTGGACAAC CAGAGGAGCC CCTTGTCCTT GGGCAAACGG 
GAGCCACCGT GGCTGCCTAG ATCCTGTCAT GGAAACTGTG ACCCAATCCA GCAGGAGGCT 
GGGGGAGGAG AGACTGTGGT GACTTTTGAG GACCGGGCCA GGCACAGGCA GAGTCAGAGG 
CGAAGCCGGC ATCGCCGAGT CAGGACTGAG GGCAAGGACT CTGCCTCTGC ATCCCGAAGC 
AGGTCTGCCA GCCAGGAGCG GAGTCTGGAT GAAGGGGTGT CCGTTGAGGG GGAGAAGGAA 
CATGAGCCTC ACAGCAGCCA CAGGAGCAAA GAGCCGACCA TCCACGAAGA GGAGAGAACC 
CAGGACTTAA GGAGGACCAA CAGTCTGATG GTCCCCAGGG GCTCTGGACT GGTAGGGGCT 
CTTGATGAAG CAGAAACACC TCTAGTCCAG CCCCAACCTG AGCTGGAAGT GGGGAAGGAT 
GCAGCTCTGA CAGAGCAGGA GGCTGAAGGC AGCAGTGAAC AAGCCCTTCT TGGGGACGTA 
CAGCTGGATG TGGGCCGGGG CATTAGCCAG AGTGAACCGG ATCTCTCCTG CATGACAGCC 
AACATGGACA AGGCTACCAC GGAGAGCACC AGTGTCACGG TCGCCATCCC TGATGTGGAC 
CCCTTGGTGG ACTCGACAGT GGTGAACATT AGCAACAAGA CGGATGGAGA AGCCAGTCCC 
TTGAAGGAGG CAGAGACTAA AGAGGAAGAA GAAGAGGTAG AGAAGAAGAA GAAGCAGAAG 
AAGGAGAAGC GAGAGACTGG AAAAGCCATG GTGCCCCATA GCTCTATGTT CATCTTCAGC 
ACCACTAACC CGATCCGCAG GGCCTGCCAC TACATTGTGA ACCTACGCTA CTTTGAAATG 
TGCATTCTCT TGGTGATTGC GGCCAGCAGC ATTGCCCTGG CAGCCGAGGA CCCTGTACTA 
ACTAACTCGG AGCGTAACAA AGTCCTGAGA TATTTCGACT ATGTTTTCAC AGGAGTGTTT 
ACCTTTGAGA TGGTTATAAA GATGATAGAC CAAGGCTTGA TCCTGCAGGA TGGATCATAC 
TTCAGAGACC TCTGGAACAT CCTGGACTTT GTAGTAGTGG TTGGTGCATT GGTAGCATTT 
GCTCTGGCGA ACGCTTTGGG AACCAACAAG GGGAGGGATA TCAAGACTAT CAAGTCTCTG 
AGGGTACTTC GAGTTCTGCG GCCACTGAAA ACCATCAAGC GCTTGCCCAA GCTGAAGGCT 
GTGTTTGACT GCGTGGTGAC CTCCTTGAAG AATGTCTTCA ACATACTCAT TGTCTACAAG 
CTCTTCATGT TCATCTTTGC CGTCATCGCT GTTCAGCTAT TCAAAGGAAA GTTCTTTTAT 
TGCACTGACA GTTCCAAGGA CACAGAGAAG GAGTGCATAG GCAACTATGT AGACCACGAG 
AAAAACAAGA TGGAGGTGAA GGGCCGGGAA TGGAAGCGTC ATGAATTCCA CTATGACAAC 
ATCATCTGGG CCCTGCTTAC CCTCTTCACA GTCTCTACAG GGGAAGGATG GCCTCAGGTT 
CTGCAGCACT CTGTAGATGT GACAGAGGAG GACAGAGGCC CAAGTCGCAG CAACCGCATG 
GAGATGTCCA TCTTTTATGT GGTCTACTTT GTGGTCTTCC CTTTCTTCTT TGTCAATATC 
TTCGTGGCTC TCATTATCAT CACCTTCCAG GAGCAAGGGG ACAAGATGAT GGAGGAGTGT 
AGCCTGGAGA AGAATGAGCG GGCATGCATC GACTTCGCCA TTAGTGCCAA ACCTCTCACC 
CGCTACATGC CACAGAACAG GCACACCTTC CAGTACCGTG TGTGGCACTT TGTAGTCTCC 
CCATCCTTTG AGTATACCAT CATGGCCATG ATTGCCTTGA ACACTGTCGT GCTGATGATG 
AAGTACTACT CTGCTCCTTG CACCTATGAG CTCGCCTTAA AGTACCTGAA CATCGCCTTC 
ACTATGGTGT TTTCCCTGGA GTGTGTCCTA AAGGTCATTG CTTTCGGCTT CCTGAACTAT 
TTCCGAGATA CCTGGAACAT CTTTGACTTC ATCACGGTGA TCGGCAGTAT CACAGAAATT 
ATCCTGACAG ATAGCAAGCT GGTGAACACC AGTGGCTTCA ATATGAGCTT TCTGAAGCTC 
TTCCGAGCTG CACGGCTCAT AAAGCTTCTG AGACAGGGCT ACACCATCCG CATCTTACTG 
TGGACCTTCG TGCAGTCCTT TAAGGCTCTT CCCTACGTCT GCCTTCTCAT TGCCATGCTT 
TTCTTCATCT ACGCCATCAT TGGGATGCAG GTGTTTGGAA ACATAAAGTT AGATGAGGAG 
AGTCACATCA ACCGGCACAA CAACTTCCGG AGTTTCTTTG GATCCCTCAT GCTGCTTTTC 
AGGAGTGCCA CGGGGGAGGC TTGGCAGGAG ATTATGCTGT CCTGTCTCGG TGAGAAGGGT 
TGTGAGCCTG ACACCACTGC ACCCTCAGGG CAGAATGAGA GTGAGCGCTG TGGCACTGAC 
CTGGCCTACG TTTATTTTGT CTCCTTCATC TTCTTCTGCT CCTTCCTGAT GCTCAACCTG 
TTCGTGGCTG TCATCATGGA CAACTTTGAG TACCTGACTC GAGATTCCTC CATCCTGGGG 
CCTCACCACT TAGATGAGTT TGTCCGTGTC TGGGCAGAAT ATGACAGAGC AGCATGTGGC 
CGCATCCATT ACACTGAGAT GTATGAAATG CTGACTCTCA TGTCACCACC GCTAGGCCTC 
GGCAAGAGAT GTCCCTCCAA AGTGGCCTAT AAGAGGCTGG TCCTGATGAA CATGCCAGTG 
GCTGAGGACA TGACAGTCCA CTTCACCTCC ACACTTATGG CTCTGATCCG GACAGCTCTG 
GACATTAAAA TCGCCAAAGG TGGTGCAGAC AGACAGCAGC TAGACTCGGA GCTGCAAAAA 
GAGACCCTGG CCATTTGGCC TCACCTGTCC CAAAAGATGT TGGATCTGCT TGTGCCCATG 
CCCAAAGCCT CTGACCTGAC TGTGGGCAAG ATCTATGCAG CAATGATGAT CATGGACTAC 
TATAAACAGA GTAAGGTAAA GAAACAGAGA CAGCAGCTGG AAGAACAGAA AAATGCACCC 
ATGTTCCAGC GCATGGAGCC CTCATCACTG CCTCAGGAGA TCATTGCTAA TGCCAAAGCC 
CTGCCTTATC TCCAGCAGGA CCCTGTTTCT GGCCTGAGTG GTCGGAGTGG ATACCCTTCA 
ATGAGTCCAC TCTCCCCTCA AGAAATATTC CAGTTGGCTT GTATGGACCC AGCGGATGAT 
GGACAATTCC AGGAACAACA ATCTCTGGTG AATGCACAAG AGCCAGAGGT TAGTGAATTA 
AAAAGTGTGC AGTCCTCTAA CCATGGCATC TACCTTCCTC CGGACACCCA GGAGCATGCG 
GGATCTGGGA GGGCATCCTC TATGCCACGT CTGACTATGG ATCCCCAGGT GGTAACAGAC 
CCTAGCTCTA TGCGACGTTC ATTTTCTACA ATTCGGGATA AGCGTTCAAA TTCCTCCTGG 
TTGGAGGAGT TCTCCATGGA ACGCAGCAGT GAAAACACTT ACAAGTCGCG GCGCAGAAGT 
TACCATTCCT CCCTGAGGCT GTCAGCACAC CGCCTGAATT CTGACTCTGG TCACAAGTCT 
GACACTCACC GATCAGGAGG CAGGGAGAGA GGCCGATCCA AAGAACGAAA GCACCTCCTC 
TCTCCAGATG TCTCCCGCTG CAATTCTGAA GAGCGAGGGA CCCAGGCCGA CTGGGAGTCC 
CCAGAACGTC GCCAGTCCAG GTCACCCAGT GAAGGAAGGT CACAGACCCC CAATAGACAG 
GGTACCGGTT CCCTAAGTGA GAGCTCCATC CCCTCTATTT CCGACACCAG CACCCCAAGA 
CGAAGCCGTC GCCAGCTCCC ACCTGTGCCA CCAAAGCCTC GTCCCCTCCT CTCCTACAGC 
TCCCTGATGA GACACACTGG CGGCATCTCT CCACCTCCTG ATGGAAGTGA GGGTGGATCC 
CCACTGGCCT CTCAAGCTCT GGAGAGCAAC AGCGCTTGCC TGACCGAGTC TTCCAACTCC 
TTGCACCCCC AGCAGGGCCA GCATCCTTCC CCACAGCACT ACATCTCCGA GCCCTATCTG 
GCCCTCCATG AAGACTCCCA CGCCTCAGAC TGTGGCGAGG AGGAGACACT CACCTTTGAG 
GCAGCCGTAG CCACTAGCTT GGGCCGGTCC AATACCATTG GCTCTGCGCC ACCCCTACGG 
CACAGCTGGC AGATGCCTAA TGGGCACTAT CGGCGGCGGA GGCGTGGGGG GCCTGGGCCT 
GGCATGATGT GTGGAGCTGT CAGTGACCTC CTGAGTGACA CGGAAGAAGA CGATAAGTGC 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Unconjugated bilirubin modulates neuronal signaling only in wild-type mice, but not after ablation of the R-type/Ca
CNS neuroscience & therapeutics(2017 Dec)
Albanna W,Neumaier F,L?ke JN,Kotliar K,Conzen C,Lindauer U,Hescheler J,Clusmann H,Schneider T,Schubert GA

miR-34c-5p functions as pronociceptive microRNA in cancer pain by targeting Cav2.3 containing calcium channels.
Pain158(9)1765-1779(2017 Sep)
Gandla J,Lomada SK,Lu J,Kuner R,Bali KK

Gender specific hippocampal whole genome transcriptome data from mice lacking the Ca
Data in brief1281-86(2017 Jun)
Papazoglou A,Henseler C,Lundt A,Wormuth C,Soos J,Broich K,Ehninger D,Weiergr?ber M

Corticothalamic Projection Neuron Development beyond Subtype Specification: Fog2 and Intersectional Controls Regulate Intraclass Neuronal Diversity.
Neuron91(1)90-106(2016 Jul)
Galazo MJ,Emsley JG,Macklis JD

Presynaptic Excitation via GABAB Receptors in Habenula Cholinergic Neurons Regulates Fear Memory Expression.
Cell166(3)716-728(2016 Jul)
Zhang J,Tan L,Ren Y,Liang J,Lin R,Feng Q,Zhou J,Hu F,Ren J,Wei C,Yu T,Zhuang Y,Bettler B,Wang F,Luo M

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Cacna1e cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

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