Cacna1b cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Cacna1b
Entrez Gene ID	12287
Full Name	calcium channel, voltage-dependent, N type, alpha 1B subunit
Synonyms	AW050276,AW060892,AW822256,BIII,Cav2.2,Cchn1a,alpha(1B)
General protein information	Preferred Names calcium channel, voltage-dependent, N type, alpha 1B subunit Names voltage-dependent N-type calcium channel subunit alpha-1B brain calcium channel III calcium channel, L type, alpha-1 polypeptide calcium channel, voltage-dependent, L type, alpha 1B subunit calcium channel, voltage-dependent, N type, alpha 1B subunit, BIII voltage-gated calcium channel subunit alpha Cav2.2
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 2 Map Location: 2 A3\|2 16.58 cM

mRNA and Protein(s)

mRNA	Protein	Name
XM_006497635.3	XP_006497698.1	voltage-dependent N-type calcium channel subunit alpha-1B isoform X13
XM_006497636.4	XP_006497699.1	voltage-dependent N-type calcium channel subunit alpha-1B isoform X14
NM_001042528.2	NP_001035993.1	voltage-dependent N-type calcium channel subunit alpha-1B isoform 1
XM_006497629.3	XP_006497692.1	voltage-dependent N-type calcium channel subunit alpha-1B isoform X6
XM_006497632.3	XP_006497695.1	voltage-dependent N-type calcium channel subunit alpha-1B isoform X10
XM_006497640.3	XP_006497703.1	voltage-dependent N-type calcium channel subunit alpha-1B isoform X18
XM_006497636.3	XP_006497699.1	voltage-dependent N-type calcium channel subunit alpha-1B isoform X14
XM_017315146.1	XP_017170635.1	voltage-dependent N-type calcium channel subunit alpha-1B isoform X9
XM_006497633.3	XP_006497696.1	voltage-dependent N-type calcium channel subunit alpha-1B isoform X11
NM_007579.3	NP_031605.2	voltage-dependent N-type calcium channel subunit alpha-1B isoform 2
XM_006497627.3	XP_006497690.1	voltage-dependent N-type calcium channel subunit alpha-1B isoform X2
XM_017315140.1	XP_017170629.1	voltage-dependent N-type calcium channel subunit alpha-1B isoform X3
XM_006497637.4	XP_006497700.1	voltage-dependent N-type calcium channel subunit alpha-1B isoform X15
XM_017315155.1	XP_017170644.1	voltage-dependent N-type calcium channel subunit alpha-1B isoform X17
XM_006497626.3	XP_006497689.1	voltage-dependent N-type calcium channel subunit alpha-1B isoform X1
XM_006497637.3	XP_006497700.1	voltage-dependent N-type calcium channel subunit alpha-1B isoform X15
XM_017315141.1	XP_017170630.1	voltage-dependent N-type calcium channel subunit alpha-1B isoform X4
XM_006497628.3	XP_006497691.1	voltage-dependent N-type calcium channel subunit alpha-1B isoform X5
XM_006497630.3	XP_006497693.1	voltage-dependent N-type calcium channel subunit alpha-1B isoform X7
XM_006497631.3	XP_006497694.1	voltage-dependent N-type calcium channel subunit alpha-1B isoform X8
XM_006497634.3	XP_006497697.1	voltage-dependent N-type calcium channel subunit alpha-1B isoform X12
XM_006497638.3	XP_006497701.1	voltage-dependent N-type calcium channel subunit alpha-1B isoform X16
XM_011239014.1	XP_011237316.1	voltage-dependent N-type calcium channel subunit alpha-1B isoform X19
XM_017315156.1	XP_017170645.1	voltage-dependent N-type calcium channel subunit alpha-1B isoform X20

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	CACNA1B	XP_520396.4
Canis lupus familiaris (dog)	CACNA1B	XP_005625483.1
Rattus norvegicus (Norway rat)	Cacna1b	NP_671482.1
Danio rerio (zebrafish)	cacna1ba	NP_001108020.1
Caenorhabditis elegans (roundworm)	unc-2	NP_001123176.1
Gallus gallus (chicken)	CACNA1B	NP_989624.1
Drosophila melanogaster (fruit fly)	cac	NP_001245636.1
Homo sapiens (human)	CACNA1B	NP_000709.1
Bos taurus (cattle)	CACNA1B	NP_777057.1
Mus musculus (house mouse)	Cacna1b	NP_001035993.1
Danio rerio (zebrafish)	cacna1bb	XP_002665969.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP002578	XP_312358.4

Gene Ontology
Bibliography

Related articles in PubMed

Cell-Specific RNA Binding Protein Rbfox2 Regulates Ca
Allen SE, Toro CP, Andrade A, L?pez-Soto EJ, Denome S, Lipscombe D
eNeuro4(5)(2017 Sep-Oct)

N-type Ca
Silva FR, Miranda AS, Santos RPM, Olmo IG, Zamponi GW, Dobransky T, Cruz JS, Vieira LB, Ribeiro FM
Neurobiology of aging551-10(2017 Jul)

High-throughput discovery of novel developmental phenotypes.
Dickinson ME, Flenniken AM, Ji X, Teboul L, Wong MD, White JK, Meehan TF, Weninger WJ, Westerberg H, Adissu H, Baker CN, Bower L, Brown JM, Caddle LB, Chiani F, Clary D, Cleak J, Daly MJ, Denegre JM, Doe B, Dolan ME, Edie SM, Fuchs H, Gailus-Durner V, Galli A, Gambadoro A, Gallegos J, Guo S, Horner NR, Hsu CW, Johnson SJ, Kalaga S, Keith LC, Lanoue L, Lawson TN, Lek M, Mark M, Marschall S, Mason J, McElwee ML, Newbigging S, Nutter LM, Peterson KA, Ramirez-Solis R, Rowland DJ, Ryder E, Samocha KE, Seavitt JR, Selloum M, Szoke-Kovacs Z, Tamura M, Trainor AG, Tudose I, Wakana S, Warren J, Wendling O, West DB, Wong L, Yoshiki A, , , , , , , , , MacArthur DG, Tocchini-Valentini GP, Gao X, Flicek P, Bradley A, Skarnes WC, Justice MJ, Parkinson HE, Moore M, Wells S, Braun RE, Svenson KL, de Angelis MH, Herault Y, Mohun T, Mallon AM, Henkelman RM, Brown SD, Adams DJ, Lloyd KC, McKerlie C, Beaudet AL, Bu?an M, Murray SA
Nature537(7621)508-514(2016 09)

Ablation of the N-type calcium channel ameliorates diabetic nephropathy with improved glycemic control and reduced blood pressure.
Ohno S, Yokoi H, Mori K, Kasahara M, Kuwahara K, Fujikura J, Naito M, Kuwabara T, Imamaki H, Ishii A, Saleem MA, Numata T, Mori Y, Nakao K, Yanagita M, Mukoyama M
Scientific reports627192(2016 Jun)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

It is essential for presynaptic neurotransmitter release.
Title: N-type Ca

Thus, GHSR1a differentially inhibits CaV2 channels by Gi/o or Gq protein pathways depending on its mode of activation.
Title: Constitutive and ghrelin-dependent GHSR1a activation impairs CaV2.1 and CaV2.2 currents in hypothalamic neurons.

Blockade of Cav2.2 in inflammatory arthritis leads to up-regulation of the osteoclast activator RANKL and concomitant joint and bone destruction.
Title: Suppression of Peripheral Pain by Blockade of Voltage-Gated Calcium 2.2 Channels in Nociceptors Induces RANKL and Impairs Recovery From Inflammatory Arthritis in a Mouse Model.

Gaba B receptors were found to mediate Cav2.2 channel inhibition.
Title: Novel mechanism of voltage-gated N-type (Cav2.2) calcium channel inhibition revealed through ?-conotoxin Vc1.1 activation of the GABA(B) receptor.

Results identified altered synaptic transmission in the olfactory system of Cav2.2-deficient mice and suggest that the olfactory system could become an attractive model to learn more about this channel and the consequences of its removal
Title: Altered synaptic transmission at olfactory and vomeronasal nerve terminals in mice lacking N-type calcium channel Cav2.2.

The following Cacna1b gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Cacna1b cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu67235	XM_011239014.1 Latest version!	Mus musculus calcium channel, voltage-dependent, N type, alpha 1B subunit (Cacna1b), transcript variant X21, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$643.30 $919.00
OMu29268	XM_006497637.4 Latest version!	Mus musculus calcium channel, voltage-dependent, N type, alpha 1B subunit (Cacna1b), transcript variant X15, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OMu29267	XM_006497636.4 Latest version!	Mus musculus calcium channel, voltage-dependent, N type, alpha 1B subunit (Cacna1b), transcript variant X14, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OMu19787	NM_007579.3 Latest version!	Mus musculus calcium channel, voltage-dependent, N type, alpha 1B subunit (Cacna1b), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1091.30 $1559.00
OMu19812	NM_001042528.2 Latest version!	Mus musculus calcium channel, voltage-dependent, N type, alpha 1B subunit (Cacna1b), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1091.30 $1559.00
OMu29257	XM_006497626.3 Latest version!	Mus musculus calcium channel, voltage-dependent, N type, alpha 1B subunit (Cacna1b), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1301.30 $1859.00
OMu29258	XM_006497627.3 Latest version!	Mus musculus calcium channel, voltage-dependent, N type, alpha 1B subunit (Cacna1b), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1301.30 $1859.00
OMu29259	XM_006497628.3 Latest version!	Mus musculus calcium channel, voltage-dependent, N type, alpha 1B subunit (Cacna1b), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1301.30 $1859.00
OMu29260	XM_006497629.3 Latest version!	Mus musculus calcium channel, voltage-dependent, N type, alpha 1B subunit (Cacna1b), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1301.30 $1859.00
OMu29261	XM_006497630.3 Latest version!	Mus musculus calcium channel, voltage-dependent, N type, alpha 1B subunit (Cacna1b), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1301.30 $1859.00
OMu29262	XM_006497631.3 Latest version!	Mus musculus calcium channel, voltage-dependent, N type, alpha 1B subunit (Cacna1b), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1091.30 $1559.00
OMu29263	XM_006497632.3 Latest version!	Mus musculus calcium channel, voltage-dependent, N type, alpha 1B subunit (Cacna1b), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1091.30 $1559.00
OMu29264	XM_006497633.3 Latest version!	Mus musculus calcium channel, voltage-dependent, N type, alpha 1B subunit (Cacna1b), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1091.30 $1559.00
OMu29265	XM_006497634.3 Latest version!	Mus musculus calcium channel, voltage-dependent, N type, alpha 1B subunit (Cacna1b), transcript variant X12, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1091.30 $1559.00
OMu29266	XM_006497635.3 Latest version!	Mus musculus calcium channel, voltage-dependent, N type, alpha 1B subunit (Cacna1b), transcript variant X13, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1091.30 $1559.00
OMu29269	XM_006497638.3 Latest version!	Mus musculus calcium channel, voltage-dependent, N type, alpha 1B subunit (Cacna1b), transcript variant X16, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$951.30 $1359.00
OMu29271	XM_006497640.3 Latest version!	Mus musculus calcium channel, voltage-dependent, N type, alpha 1B subunit (Cacna1b), transcript variant X20, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$811.30 $1159.00
OMu80051	XM_017315140.1 Latest version!	Mus musculus calcium channel, voltage-dependent, N type, alpha 1B subunit (Cacna1b), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1301.30 $1859.00
OMu80052	XM_017315141.1 Latest version!	Mus musculus calcium channel, voltage-dependent, N type, alpha 1B subunit (Cacna1b), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1301.30 $1859.00
OMu80053	XM_017315146.1 Latest version!	Mus musculus calcium channel, voltage-dependent, N type, alpha 1B subunit (Cacna1b), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1091.30 $1559.00
OMu80054	XM_017315155.1 Latest version!	Mus musculus calcium channel, voltage-dependent, N type, alpha 1B subunit (Cacna1b), transcript variant X18, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$951.30 $1359.00
OMu80055	XM_017315156.1 Latest version!	Mus musculus calcium channel, voltage-dependent, N type, alpha 1B subunit (Cacna1b), transcript variant X22, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1301.30 $1859.00
View Old Accession Versions >>
OMu29267	XM_006497636.3	Mus musculus calcium channel, voltage-dependent, N type, alpha 1B subunit (Cacna1b), transcript variant X14, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1091.30 $1559.00
OMu29268	XM_006497637.3	Mus musculus calcium channel, voltage-dependent, N type, alpha 1B subunit (Cacna1b), transcript variant X15, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$951.30 $1359.00
Hide Old Accession Versions >>

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***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Cacna1b ( XM_011239014.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011237316.1 Mus musculus calcium channel, voltage-dependent, N type, alpha 1B subunit (Cacna1b), transcript variant X21, mRNA.

Price & Availability↑

CloneID	OMu67235
Clone ID Related Accession (Same CDS sequence)	XM_011239014.1
Accession Version	XM_011239014.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3729bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	voltage-dependent N-type calcium channel subunit alpha-1B isoform X19
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAACCTGG AAGGCCAAGC AGAGGGCAAG AAGGAGGCAG AGGCGGATGA TGTGCTGAGA 
AGAGGCCCCA GGCCCATCGT TCCCTACAGC TCCATGTTTT GTCTCAGCCC CACCAACCTG 
CTTCGTCGCT TCTGCCATTA CATTGTGACC ATGCGGTACT TTGAGATGGT AATTCTTGTG 
GTCATTGCCT TGAGCAGCAT TGCCCTGGCT GCAGAGGATC CTGTGCGGAC AGATTCATTC 
AGGAACAACG CTTTAAAGTA CATGGATTAC ATCTTTACAG GAGTCTTCAC CTTTGAAATG 
GTCATAAAGA TGATAGACTT GGGCTTGCTG CTGCACCCTG GTGCCTACTT CCGGGACCTG 
TGGAACATTC TGGACTTCAT TGTTGTCAGT GGAGCCCTGG TGGCATTTGC GTTCTCGAGC 
TTCATGGGAG GATCCAAAGG GAAAGACATC AATACCATCA AGTCTCTGAG AGTCCTGCGT 
GTCCTGAGGC CCCTCAAGAC CATCAAGCGG CTGCCTAAGC TCAAGGCTGT CTTTGACTGT 
GTGGTGAACT CCCTGAAGAA CGTCTTGAAC ATCCTGATTG TCTACATGCT CTTCATGTTC 
ATATTTGCCG TCATTGCCGT CCAGCTCTTC AAAGGGAAGT TCTTTTACTG TACTGATGAA 
TCCAAGGAGC TGGAGAGGGA CTGCCGGGGT CAGTATTTGG ATTATGAGAA GGAAGAAGTA 
GAAGCCCAGC CAAGGCAGTG GAAGAAATAT GACTTCCACT ATGACAATGT TCTCTGGGCC 
TTGTTGACGC TGTTCACAGT GTCCACGGGA GAGGGGTGGC CCATGGTGCT GAAACACTCT 
GTGGATGCCA CCTATGAGGA ACAGGGGCCC AGTCCCGGGT TCCGGATGGA GCTCTCCATC 
TTCTACGTGG TCTACTTTGT GGTCTTCCCT TTTTTCTTTG TCAACATCTT TGTGGCCTTG 
ATCATTATCA CCTTCCAGGA ACAGGGAGAT AAGGTGATGT CTGAATGCAG CTTAGAAAAG 
AATGAGAGGG CTTGCATTGA TTTTGCCATC AGTGCCAAGC CCCTGACACG GTACATGCCT 
CAAAACAAAC AGTCGTTCCA GTATAAGACA TGGACATTCG TGGTCTCTCC ACCCTTTGAG 
TACTTCATCA TGGCTATGAT AGCCCTCAAC ACAGTGGTGC TGATGATGAA GTTCTATGAT 
GCACCTTATG AGTACGAGCT GATGCTGAAA TGCCTGAACA TTGTCTTCAC ATCCATGTTC 
TCGATGGAGT GCATACTGAA GATCATCGCC TTTGGGGTAT TGAACTACTT CAGAGATGCC 
TGGAACGTCT TTGACTTTGT CACGGTTTTG GGAAGTATTA CTGATATTTT AGTAACAGAG 
ATAGCGGAAA CGAACAACTT CATCAACCTA AGCTTCCTTC GCCTCTTCCG GGCGGCACGG 
CTGATCAAGC TGCTTCGCCA GGGCTACACC ATCCGCATCC TATTGTGGAC CTTCGTCCAG 
TCCTTTAAGG CGCTGCCCTA CGTGTGCCTC CTCATTGCCA TGCTGTTCTT CATCTACGCC 
ATCATCGGCA TGCAGGTTTT TGGAAACATT GCCCTTGATG ATGACACCAG TATCAACCGA 
CACAACAACT TCCGGACATT TCTGCAAGCC TTAATGCTAT TGTTCAGGAG TGCCACTGGG 
GAGGCCTGGC ATGAGATCAT GCTGTCTTGT CTGGGCAACC GGGCCTGTGA CCCACATGCC 
AACGCCAGTG AGTGCGGGAG CGACTTTGCC TATTTTTATT TTGTCTCCTT CATCTTCCTC 
TGTTCCTTTC TGATGTTGAA CCTCTTTGTT GCTGTAATCA TGGACAATTT TGAGTACCTC 
ACTCGGGACT CTTCCATCCT AGGGCCTCAC CACTTAGACG AATTCATTCG AGTCTGGGCT 
GAATACGACC CAGCTGCGTG TGGGCGCATC AGTTACAATG ACATGTTTGA GATGCTGAAA 
CACATGTCCC CACCTCTGGG GTTGGGGAAG AAATGCCCGG CTCGAGTTGC ATACAAGCGC 
CTGGTTCGCA TGAACATGCC CATATCCAAT GAGGACATGA CGGTGCACTT TACGTCCACA 
CTGATGGCCC TCATCCGGAC AGCACTGGAG ATCAAGCTTG CCCCAGCGGG GACGAAGCAG 
CACCAGTGTG ATGCTGAGCT GAGAAAAGAG ATCTCTTCTG TGTGGGCTAA TCTGCCCCAG 
AAGACTCTGG ACTTACTGGT ACCACCCCAC AAACCTGACG AGATGACAGT GGGGAAGGTC 
TATGCTGCTC TCATGATATT TGACTTCTAC AAACAGAACA AAACCACCAG AGATCAGACT 
CACCAAGCTC CCGGAGGCCT GTCCCAGATG GGTCCCGTTT CCCTGTTCCA CCCTCTGAAG 
GCCACCCTGG AACAGACACA GCCCGCTGTG CTTCGAGGAG CTCGGGTTTT CCTTCGGCAA 
AAGAGTGCAA CTTCCCTCAG CAATGGGGGT GCCATACAAA CCCAGGAAAG TGGCATCAAG 
GAGTCGCTGT CCTGGGGCAC GCAGAGGACC CAAGATGCAC TTTATGAGGC CAGAGCACCT 
CTAGAACGTG GCCATTCTGC AGAGATCCCT GTGGGGCAGT CAGGAACACT GGCTGTGGAT 
GTCCAGATGC AGAACATGAC ACTGAGAGGA CCAGATGGGG AGCCCCAGCC TGGGCTGGAA 
AGCCAAGGCA GAGCTGCCTC TATGCCACGC CTAGCGGCAG AAACACAGCC GGCCCCTAAT 
GCCAGCCCCA TGAAGCGCTC CATCTCCACA CTGGCTCCAC GCCCACATGG GACTCAGCTT 
TGCAGCACAG TTCTGGACCG GCCTCCTCCT AGCCAGGCAT CACATCACCA CCACCACCGC 
TGCCACCGGC GCAGAGACAA GAAGCAAAGG TCCCTGGAAA AGGGGCCCAG CCTGTCTGTT 
GACCCAGAAG GTGCACCAAG CACTGCTGCA GGACCTGGTC TGCCCCATGG AGAAGGATCC 
ACCGCCTGCC GGCGGGACCG TAAACAGGAG CGAGGCCGGT CCCAGGAGCG GAGGCAGCCC 
TCATCTTCCT CTTCAGAGAA GCAGCGCTTC TATTCCTGTG ACCGCTTTGG GAGCCGGGAG 
CCCCCGCAAC TGATGCCCTC ACTCAGTAGC CACCCCACAT CGCCAACAGC GGCGCTAGAG 
CCAGCACCCC ACCCACAGGG CAGTGGTTCC GTTAATGGGA GCCCCTTGAT GTCAACATCC 
GGTGCTAGCA CCCCGGGCCG AGGTGGGCGG AGGCAGCTCC CCCAGACTCC TCTGACCCCA 
CGCCCCAGCA TCACCTACAA GACCGCCAAT TCCTCGCCTG TCCACTTTGC TGAGGGTCAG 
AGCGGCCTCC CAGCCTTCTC CCCTGGCCGT CTCAGCCGCG GCCTTTCTGA ACACAATGCC 
CTGCTCCAGA AAGAGCCCCT GAGCCAGCCT CTAGCTCCTG GCTCCCGAAT TGGCTCTGAC 
CCTTACCTAG GGCAGCGTCT GGACAGTGAG GCCTCCGCCC ACACCCTGCC TGAGGATACA 
CTCACCTTTG AAGAGGCAGT GGCCACCAAC TCTGGCCGCT CCTCCAGGAC TTCCTATGTG 
TCCTCCCTCA CTTCCCAATC CCACCCTCTC CGCCGTGTAC CCAATGGCTA TCACTGCACT 
TTGGGACTCA GCACTGGCGT CCGGGCGCGG CACAGCTACC ACCACCCCGA TCAGGACCAC 
TGGTGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011237316.1
CDS	311..4039
Translation	MNLEGQAEGKKEAEADDVLRRGPRPIVPYSSMFCLSPTNLLRRFCHYIVTMRYFEMVILVVIALSSIALAAEDPVRTDSFRNNALKYMDYIFTGVFTFEMVIKMIDLGLLLHPGAYFRDLWNILDFIVVSGALVAFAFSSFMGGSKGKDINTIKSLRVLRVLRPLKTIKRLPKLKAVFDCVVNSLKNVLNILIVYMLFMFIFAVIAVQLFKGKFFYCTDESKELERDCRGQYLDYEKEEVEAQPRQWKKYDFHYDNVLWALLTLFTVSTGEGWPMVLKHSVDATYEEQGPSPGFRMELSIFYVVYFVVFPFFFVNIFVALIIITFQEQGDKVMSECSLEKNERACIDFAISAKPLTRYMPQNKQSFQYKTWTFVVSPPFEYFIMAMIALNTVVLMMKFYDAPYEYELMLKCLNIVFTSMFSMECILKIIAFGVLNYFRDAWNVFDFVTVLGSITDILVTEIAETNNFINLSFLRLFRAARLIKLLRQGYTIRILLWTFVQSFKALPYVCLLIAMLFFIYAIIGMQVFGNIALDDDTSINRHNNFRTFLQALMLLFRSATGEAWHEIMLSCLGNRACDPHANASECGSDFAYFYFVSFIFLCSFLMLNLFVAVIMDNFEYLTRDSSILGPHHLDEFIRVWAEYDPAACGRISYNDMFEMLKHMSPPLGLGKKCPARVAYKRLVRMNMPISNEDMTVHFTSTLMALIRTALEIKLAPAGTKQHQCDAELRKEISSVWANLPQKTLDLLVPPHKPDEMTVGKVYAALMIFDFYKQNKTTRDQTHQAPGGLSQMGPVSLFHPLKATLEQTQPAVLRGARVFLRQKSATSLSNGGAIQTQESGIKESLSWGTQRTQDALYEARAPLERGHSAEIPVGQSGTLAVDVQMQNMTLRGPDGEPQPGLESQGRAASMPRLAAETQPAPNASPMKRSISTLAPRPHGTQLCSTVLDRPPPSQASHHHHHRCHRRRDKKQRSLEKGPSLSVDPEGAPSTAAGPGLPHGEGSTACRRDRKQERGRSQERRQPSSSSSEKQRFYSCDRFGSREPPQLMPSLSSHPTSPTAALEPAPHPQGSGSVNGSPLMSTSGASTPGRGGRRQLPQTPLTPRPSITYKTANSSPVHFAEGQSGLPAFSPGRLSRGLSEHNALLQKEPLSQPLAPGSRIGSDPYLGQRLDSEASAHTLPEDTLTFEEAVATNSGRSSRTSYVSSLTSQSHPLRRVPNGYHCTLGLSTGVRARHSYHHPDQDHWC

Target ORF information:

RefSeq Version	XM_011239014.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus calcium channel, voltage-dependent, N type, alpha 1B subunit (Cacna1b), transcript variant X21, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011239014.1

ORF Insert Sequence:

ATGAACCTGG AAGGCCAAGC AGAGGGCAAG AAGGAGGCAG AGGCGGATGA TGTGCTGAGA 
AGAGGCCCCA GGCCCATCGT TCCCTACAGC TCCATGTTTT GTCTCAGCCC CACCAACCTG 
CTTCGTCGCT TCTGCCATTA CATTGTGACC ATGCGGTACT TTGAGATGGT AATTCTTGTG 
GTCATTGCCT TGAGCAGCAT TGCCCTGGCT GCAGAGGATC CTGTGCGGAC AGATTCATTC 
AGGAACAACG CTTTAAAGTA CATGGATTAC ATCTTTACAG GAGTCTTCAC CTTTGAAATG 
GTCATAAAGA TGATAGACTT GGGCTTGCTG CTGCACCCTG GTGCCTACTT CCGGGACCTG 
TGGAACATTC TGGACTTCAT TGTTGTCAGT GGAGCCCTGG TGGCATTTGC GTTCTCGAGC 
TTCATGGGAG GATCCAAAGG GAAAGACATC AATACCATCA AGTCTCTGAG AGTCCTGCGT 
GTCCTGAGGC CCCTCAAGAC CATCAAGCGG CTGCCTAAGC TCAAGGCTGT CTTTGACTGT 
GTGGTGAACT CCCTGAAGAA CGTCTTGAAC ATCCTGATTG TCTACATGCT CTTCATGTTC 
ATATTTGCCG TCATTGCCGT CCAGCTCTTC AAAGGGAAGT TCTTTTACTG TACTGATGAA 
TCCAAGGAGC TGGAGAGGGA CTGCCGGGGT CAGTATTTGG ATTATGAGAA GGAAGAAGTA 
GAAGCCCAGC CAAGGCAGTG GAAGAAATAT GACTTCCACT ATGACAATGT TCTCTGGGCC 
TTGTTGACGC TGTTCACAGT GTCCACGGGA GAGGGGTGGC CCATGGTGCT GAAACACTCT 
GTGGATGCCA CCTATGAGGA ACAGGGGCCC AGTCCCGGGT TCCGGATGGA GCTCTCCATC 
TTCTACGTGG TCTACTTTGT GGTCTTCCCT TTTTTCTTTG TCAACATCTT TGTGGCCTTG 
ATCATTATCA CCTTCCAGGA ACAGGGAGAT AAGGTGATGT CTGAATGCAG CTTAGAAAAG 
AATGAGAGGG CTTGCATTGA TTTTGCCATC AGTGCCAAGC CCCTGACACG GTACATGCCT 
CAAAACAAAC AGTCGTTCCA GTATAAGACA TGGACATTCG TGGTCTCTCC ACCCTTTGAG 
TACTTCATCA TGGCTATGAT AGCCCTCAAC ACAGTGGTGC TGATGATGAA GTTCTATGAT 
GCACCTTATG AGTACGAGCT GATGCTGAAA TGCCTGAACA TTGTCTTCAC ATCCATGTTC 
TCGATGGAGT GCATACTGAA GATCATCGCC TTTGGGGTAT TGAACTACTT CAGAGATGCC 
TGGAACGTCT TTGACTTTGT CACGGTTTTG GGAAGTATTA CTGATATTTT AGTAACAGAG 
ATAGCGGAAA CGAACAACTT CATCAACCTA AGCTTCCTTC GCCTCTTCCG GGCGGCACGG 
CTGATCAAGC TGCTTCGCCA GGGCTACACC ATCCGCATCC TATTGTGGAC CTTCGTCCAG 
TCCTTTAAGG CGCTGCCCTA CGTGTGCCTC CTCATTGCCA TGCTGTTCTT CATCTACGCC 
ATCATCGGCA TGCAGGTTTT TGGAAACATT GCCCTTGATG ATGACACCAG TATCAACCGA 
CACAACAACT TCCGGACATT TCTGCAAGCC TTAATGCTAT TGTTCAGGAG TGCCACTGGG 
GAGGCCTGGC ATGAGATCAT GCTGTCTTGT CTGGGCAACC GGGCCTGTGA CCCACATGCC 
AACGCCAGTG AGTGCGGGAG CGACTTTGCC TATTTTTATT TTGTCTCCTT CATCTTCCTC 
TGTTCCTTTC TGATGTTGAA CCTCTTTGTT GCTGTAATCA TGGACAATTT TGAGTACCTC 
ACTCGGGACT CTTCCATCCT AGGGCCTCAC CACTTAGACG AATTCATTCG AGTCTGGGCT 
GAATACGACC CAGCTGCGTG TGGGCGCATC AGTTACAATG ACATGTTTGA GATGCTGAAA 
CACATGTCCC CACCTCTGGG GTTGGGGAAG AAATGCCCGG CTCGAGTTGC ATACAAGCGC 
CTGGTTCGCA TGAACATGCC CATATCCAAT GAGGACATGA CGGTGCACTT TACGTCCACA 
CTGATGGCCC TCATCCGGAC AGCACTGGAG ATCAAGCTTG CCCCAGCGGG GACGAAGCAG 
CACCAGTGTG ATGCTGAGCT GAGAAAAGAG ATCTCTTCTG TGTGGGCTAA TCTGCCCCAG 
AAGACTCTGG ACTTACTGGT ACCACCCCAC AAACCTGACG AGATGACAGT GGGGAAGGTC 
TATGCTGCTC TCATGATATT TGACTTCTAC AAACAGAACA AAACCACCAG AGATCAGACT 
CACCAAGCTC CCGGAGGCCT GTCCCAGATG GGTCCCGTTT CCCTGTTCCA CCCTCTGAAG 
GCCACCCTGG AACAGACACA GCCCGCTGTG CTTCGAGGAG CTCGGGTTTT CCTTCGGCAA 
AAGAGTGCAA CTTCCCTCAG CAATGGGGGT GCCATACAAA CCCAGGAAAG TGGCATCAAG 
GAGTCGCTGT CCTGGGGCAC GCAGAGGACC CAAGATGCAC TTTATGAGGC CAGAGCACCT 
CTAGAACGTG GCCATTCTGC AGAGATCCCT GTGGGGCAGT CAGGAACACT GGCTGTGGAT 
GTCCAGATGC AGAACATGAC ACTGAGAGGA CCAGATGGGG AGCCCCAGCC TGGGCTGGAA 
AGCCAAGGCA GAGCTGCCTC TATGCCACGC CTAGCGGCAG AAACACAGCC GGCCCCTAAT 
GCCAGCCCCA TGAAGCGCTC CATCTCCACA CTGGCTCCAC GCCCACATGG GACTCAGCTT 
TGCAGCACAG TTCTGGACCG GCCTCCTCCT AGCCAGGCAT CACATCACCA CCACCACCGC 
TGCCACCGGC GCAGAGACAA GAAGCAAAGG TCCCTGGAAA AGGGGCCCAG CCTGTCTGTT 
GACCCAGAAG GTGCACCAAG CACTGCTGCA GGACCTGGTC TGCCCCATGG AGAAGGATCC 
ACCGCCTGCC GGCGGGACCG TAAACAGGAG CGAGGCCGGT CCCAGGAGCG GAGGCAGCCC 
TCATCTTCCT CTTCAGAGAA GCAGCGCTTC TATTCCTGTG ACCGCTTTGG GAGCCGGGAG 
CCCCCGCAAC TGATGCCCTC ACTCAGTAGC CACCCCACAT CGCCAACAGC GGCGCTAGAG 
CCAGCACCCC ACCCACAGGG CAGTGGTTCC GTTAATGGGA GCCCCTTGAT GTCAACATCC 
GGTGCTAGCA CCCCGGGCCG AGGTGGGCGG AGGCAGCTCC CCCAGACTCC TCTGACCCCA 
CGCCCCAGCA TCACCTACAA GACCGCCAAT TCCTCGCCTG TCCACTTTGC TGAGGGTCAG 
AGCGGCCTCC CAGCCTTCTC CCCTGGCCGT CTCAGCCGCG GCCTTTCTGA ACACAATGCC 
CTGCTCCAGA AAGAGCCCCT GAGCCAGCCT CTAGCTCCTG GCTCCCGAAT TGGCTCTGAC 
CCTTACCTAG GGCAGCGTCT GGACAGTGAG GCCTCCGCCC ACACCCTGCC TGAGGATACA 
CTCACCTTTG AAGAGGCAGT GGCCACCAAC TCTGGCCGCT CCTCCAGGAC TTCCTATGTG 
TCCTCCCTCA CTTCCCAATC CCACCCTCTC CGCCGTGTAC CCAATGGCTA TCACTGCACT 
TTGGGACTCA GCACTGGCGT CCGGGCGCGG CACAGCTACC ACCACCCCGA TCAGGACCAC 
TGGTGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cacna1b ( XM_006497637.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006497700.1 Mus musculus calcium channel, voltage-dependent, N type, alpha 1B subunit (Cacna1b), transcript variant X15, mRNA.

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CloneID	OMu29268
Clone ID Related Accession (Same CDS sequence)	XM_006497637.4 , XM_006497637.3
Accession Version	XM_006497637.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5427bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	voltage-dependent N-type calcium channel subunit alpha-1B isoform X15
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006497637.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTCCGCT TCGGGGACGA GCTAGGCGGC CGCTATGGGG GCACCGGCGG CGGGGAGCGG 
GCTCGGGGTG GCGGGGCCGG CGGGGCGGGT GGCCCGGGCC AGGGGGGTCT GCCGCCGGGC 
CAGCGGGTCC TGTACAAGCA GTCCATTGCG CAGCGCGCAC GGACTATGGC CCTGTACAAC 
CCCATCCCAG TCAAGCAGAA CTGCTTCACC GTCAACCGCT CGCTCTTCGT CTTCAGCGAG 
GACAACGTCG TCCGCAAATA CGCTAAGCGC ATCACCGAAT GGCCGCCCTT CGAATACATG 
ATCCTGGCCA CCATCATCGC CAACTGCATT GTTCTGGCCC TGGAGCAGCA CCTCCCTGAT 
GGGGACAAGA CTCCCATGTC TGAGCGACTA GATGACACGG AGCCTTACTT CATCGGGATC 
TTTTGCTTCG AGGCGGGCAT CAAGATCATA GCCCTGGGCT TTGTTTTCCA CAAGGGCTCC 
TACCTTCGGA ACGGCTGGAA TGTCATGGAC TTCGTGGTGG TCCTCACGGG GATTCTCGCC 
ACAGCTGGAA CTGACTTTGA CCTGCGCACA CTGAGGGCTG TGCGTGTGCT TAGGCCCCTG 
AAGCTGGTGT CTGGAATTCC AAGCTTGCAG GTGGTGCTTA AGTCCATCAT GAAGGCCATG 
GTCCCGCTGC TGCAGATTGG GCTGCTGCTC TTCTTTGCCA TCCTCATGTT TGCCATCATC 
GGCCTCGAAT TCTATATGGG CAAATTCCAT AAGGCCTGTT TCCCCAACAG CACAGATACA 
GAGCCTGTGG GTGACTTTCC CTGTGGCAAA GATCCCCCTG CTCGTCAGTG TGATGGTGAC 
ACCGAATGCC GGGAGTACTG GCCAGGACCC AACTTTGGCA TCACCAATTT TGACAACATC 
CTGTTTGCCA TCTTGACAGT GTTCCAGTGT ATCACCATGG AGGGCTGGAC TGACATCCTC 
TACAATACAA ATGATGCGGC TGGCAACACA TGGAACTGGT TGTACTTCAT CCCCCTCATC 
ATCATTGGCT CCTTCTTCAT GCTCAACCTG GTGCTGGGTG TGCTTTCCGG AGAGTTTGCC 
AAGGAGCGGG AGCGAGTCGA GAACCGCCGC GCCTTCCTGA AGCTCCGCAG GCAGCAGCAG 
ATTGAGCGAG AGCTGAATGG GTACTTGGAG TGGATCTTCA AGGCAGAGGA AGTCATGTTG 
GCAGAGGAGG ACAAGAATGC AGAAGAGAAA TCCCCTTTGG ATGCAGTGTT GAAGAGAGCT 
GCCACCAAGA AGAGCCGAAA TGACCTCATC CATGCAGAAG AGGGGGAGGA CCGGTTTGTA 
GACCTCTGTG CAGTTGGGTC TCCATTTGCT CGTGCCAGCC TCAAGAGTGG GAAGACGGAG 
AGCTCATCGT ACTTCCGGAG AAAGGAGAAG ATGTTCCGGT TCTTTATCCG GCGTATGGTG 
AAAGCACAGA GCTTCTACTG GGTGGTACTG TGTGTGGTGG CCCTGAACAC ACTGTGTGTG 
GCCATGGTGC ACTATAATCA GCCTCAGCGG CTTACCACTG CACTGTACTT TGCAGAGTTT 
GTTTTCCTGG GTCTCTTCCT CACAGAGATG TCCCTGAAGA TGTATGGCCT AGGGCCCAGA 
AGTTACTTCA GGTCTTCCTT CAACTGCTTT GACTTTGGGG TGATTGTGGG GAGTATCTTT 
GAAGTAGTCT GGGCTGCCAT CAAGCCAGGA ACCTCCTTTG GAATCAGTGT GCTGCGGGCT 
CTGCGACTGC TGAGGATATT CAAAGTTACC AAGTATTGGA ACTCTCTGAG GAACCTGGTG 
GTTTCCCTCC TCAATTCCAT GAAGTCCATC ATCAGCCTTC TCTTCCTGCT TTTCCTCTTC 
ATCGTGGTCT TCGCTCTGTT GGGGATGCAG CTGTTTGGGG GACAGTTCAA CTTTCAAGAT 
GAGACTCCAA CCACCAATTT TGATACCTTC CCAGCTGCCA TCCTCACTGT CTTTCAGATC 
CTGACAGGAG AGGATTGGAA TGCCGTAATG TATCATGGGA TTGAGTCGCA AGGTGGAGTC 
AGCAAAGGCA TGTTTTCTTC CTTTTACTTC ATCGTCCTGA CACTGTTTGG AAACTACACC 
CTGCTGAATG TTTTTCTGGC CATTGCTGTG GACAACCTTG CCAATGCCCA GGAGTTGACC 
AAGGATGAAG AGGAGATGGA AGAAGCAGCC AATCAGAAAC TTGCTCTTCA GAAGGCCAAA 
GAAGTAGCTG AAGTCAGCCC CATGTCTGCT GCCAATATCT CCATCGCTGC TTTTGTAAAG 
CAAACTCGAG GTACTGTATC TCGCAGCTCA TCTGTCTCCA GCGTAAACTC ACCCAGGCAG 
CAGAACTCGG CCAAGGCGCG CTCAGTATGG GAGCAGCGGG CCAGTCAGCT AAGGCTCCAG 
AATCTGCGTG CCAGCTGTGA GGCATTGTAC AGTGAGATGG ACCCTGAGGA GCGCCTGCGT 
TATGCCAGCA CGCGCCATGT GAGGCCAGAC ATGAAGACAC ACATGGACCG ACCCCTAGTG 
GTGGAGCCTG GTCGAGATGG CTTGCGGGGA CCCGTTGGGA GCAAGTCAAA GCCTGAAGGC 
ACGGAGGCCA CAGAAAGCGC GGACCTACCT CGCAGGCACC ACCGGCACCG TGATAGGGAC 
AAGACCTCAG CCACAGCACC TGCTGGAGGC GAACAGGACA GGACAGAAAG CACCGAGACC 
GGGGCCCGGG AGGAACGTGC GCGCCCTCGT CGAAGTCACA GCAAGGAGAC TCCAGGGGCT 
GACACGCAAG TGCGCTGTGA GCGCAGTAGA CGTCACCACC GGCGCGGCTC CCCGGAGGAG 
GCCACTGAAC GGGAGCCTCG GCGCCACCGT GCCCACCGGC ATGCACAGGA CTCAAGCAAG 
GAGGGCACGG CGCCGGTGCT TGTACCCAAG GGTGAGCGAC GAGCAAGACA CCGAGGCCCA 
CGCACGGGTC CACGTGAGGC AGAGAACAAC GAGGAGCCCA CACGCAGGCA CCGTGCAAGG 
CATAAGGTGC CACCCACACT GCAGCCCCCA GAGAGGGAGG CTGCAGAGAA GGAGAGCAAC 
GCGGTGGAAG GGGATAAGGA AACCCGAAAT CACCAGCCCA AGGAACCTCA CTGTGACCTG 
GAGGCCATTG CAGTTACAGG TGTGGGCCCT CTGCACATGC TGCCCAGCAC CTGTCTCCAG 
AAAGTGGACG AGCAACCAGA GGATGCAGAC AACCAGCGTA ATGTCACCCG GATGGGCAGT 
CAGCCCTCAG ATCCCAGCAC CACTGTGCAT GTCCCAGTGA CACTGACAGG CCCTCCTGGG 
GAGACCCCTG TAGTTCCCAG TGGTAACATG AACCTGGAAG GCCAAGCAGA GGGCAAGAAG 
GAGGCAGAGG CGGATGATGT GCTGAGAAGA GGCCCCAGGC CCATCGTTCC CTACAGCTCC 
ATGTTTTGTC TCAGCCCCAC CAACCTGCTT CGTCGCTTCT GCCATTACAT TGTGACCATG 
CGGTACTTTG AGATGGTAAT TCTTGTGGTC ATTGCCTTGA GCAGCATTGC CCTGGCTGCA 
GAGGATCCTG TGCGGACAGA TTCATTCAGG AACAACGCTT TAAAGTACAT GGATTACATC 
TTTACAGGAG TCTTCACCTT TGAAATGGTC ATAAAGATGA TAGACTTGGG CTTGCTGCTG 
CACCCTGGTG CCTACTTCCG GGACCTGTGG AACATTCTGG ACTTCATTGT TGTCAGTGGA 
GCCCTGGTGG CATTTGCGTT CTCGAGCTTC ATGGGAGGAT CCAAAGGGAA AGACATCAAT 
ACCATCAAGT CTCTGAGAGT CCTGCGTGTC CTGAGGCCCC TCAAGACCAT CAAGCGGCTG 
CCTAAGCTCA AGGCTGTCTT TGACTGTGTG GTGAACTCCC TGAAGAACGT CTTGAACATC 
CTGATTGTCT ACATGCTCTT CATGTTCATA TTTGCCGTCA TTGCCGTCCA GCTCTTCAAA 
GGGAAGTTCT TTTACTGTAC TGATGAATCC AAGGAGCTGG AGAGGGACTG CCGGGGTCAG 
TATTTGGATT ATGAGAAGGA AGAAGTAGAA GCCCAGCCAA GGCAGTGGAA GAAATATGAC 
TTCCACTATG ACAATGTTCT CTGGGCCTTG TTGACGCTGT TCACAGTGTC CACGGGAGAG 
GGGTGGCCCA TGGTGCTGAA ACACTCTGTG GATGCCACCT ATGAGGAACA GGGGCCCAGT 
CCCGGGTTCC GGATGGAGCT CTCCATCTTC TACGTGGTCT ACTTTGTGGT CTTCCCTTTT 
TTCTTTGTCA ACATCTTTGT GGCCTTGATC ATTATCACCT TCCAGGAACA GGGAGATAAG 
GTGATGTCTG AATGCAGCTT AGAAAAGAAT GAGAGGGCTT GCATTGATTT TGCCATCAGT 
GCCAAGCCCC TGACACGGTA CATGCCTCAA AACAAACAGT CGTTCCAGTA TAAGACATGG 
ACATTCGTGG TCTCTCCACC CTTTGAGTAC TTCATCATGG CTATGATAGC CCTCAACACA 
GTGGTGCTGA TGATGAAGTT CTATGATGCA CCTTATGAGT ACGAGCTGAT GCTGAAATGC 
CTGAACATTG TCTTCACATC CATGTTCTCG ATGGAGTGCA TACTGAAGAT CATCGCCTTT 
GGGGTATTGA ACTACTTCAG AGATGCCTGG AACGTCTTTG ACTTTGTCAC GGTTTTGGGA 
AGTATTACTG ATATTTTAGT AACAGAGATA GCGGAAACGA ACAACTTCAT CAACCTAAGC 
TTCCTTCGCC TCTTCCGGGC GGCACGGCTG ATCAAGCTGC TTCGCCAGGG CTACACCATC 
CGCATCCTAT TGTGGACCTT CGTCCAGTCC TTTAAGGCGC TGCCCTACGT GTGCCTCCTC 
ATTGCCATGC TGTTCTTCAT CTACGCCATC ATCGGCATGC AGGTTTTTGG AAACATTGCC 
CTTGATGATG ACACCAGTAT CAACCGACAC AACAACTTCC GGACATTTCT GCAAGCCTTA 
ATGCTATTGT TCAGGAGTGC CACTGGGGAG GCCTGGCATG AGATCATGCT GTCTTGTCTG 
GGCAACCGGG CCTGTGACCC ACATGCCAAC GCCAGTGAGT GCGGGAGCGA CTTTGCCTAT 
TTTTATTTTG TCTCCTTCAT CTTCCTCTGT TCCTTTCTGA TGTTGAACCT CTTTGTTGCT 
GTAATCATGG ACAATTTTGA GTACCTCACT CGGGACTCTT CCATCCTAGG GCCTCACCAC 
TTAGACGAAT TCATTCGAGT CTGGGCTGAA TACGACCCAG CTGCGTGCCA GGAGAATCTC 
AGCTCAGATG AGAGTGGGGT CCGGGAGGAG AGAATGCTGG CAAAACTTCC ACCCACCCAG 
CCTTCTGGAC CAGGCACCTG CCGGGACCAC TCTCAGGACG CCAAGCCAAG CTCCAGAGCC 
CAAACACTAA TTCTAGGGTG GGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006497700.1
CDS	153..5579
Translation	MVRFGDELGGRYGGTGGGERARGGGAGGAGGPGQGGLPPGQRVLYKQSIAQRARTMALYNPIPVKQNCFTVNRSLFVFSEDNVVRKYAKRITEWPPFEYMILATIIANCIVLALEQHLPDGDKTPMSERLDDTEPYFIGIFCFEAGIKIIALGFVFHKGSYLRNGWNVMDFVVVLTGILATAGTDFDLRTLRAVRVLRPLKLVSGIPSLQVVLKSIMKAMVPLLQIGLLLFFAILMFAIIGLEFYMGKFHKACFPNSTDTEPVGDFPCGKDPPARQCDGDTECREYWPGPNFGITNFDNILFAILTVFQCITMEGWTDILYNTNDAAGNTWNWLYFIPLIIIGSFFMLNLVLGVLSGEFAKERERVENRRAFLKLRRQQQIERELNGYLEWIFKAEEVMLAEEDKNAEEKSPLDAVLKRAATKKSRNDLIHAEEGEDRFVDLCAVGSPFARASLKSGKTESSSYFRRKEKMFRFFIRRMVKAQSFYWVVLCVVALNTLCVAMVHYNQPQRLTTALYFAEFVFLGLFLTEMSLKMYGLGPRSYFRSSFNCFDFGVIVGSIFEVVWAAIKPGTSFGISVLRALRLLRIFKVTKYWNSLRNLVVSLLNSMKSIISLLFLLFLFIVVFALLGMQLFGGQFNFQDETPTTNFDTFPAAILTVFQILTGEDWNAVMYHGIESQGGVSKGMFSSFYFIVLTLFGNYTLLNVFLAIAVDNLANAQELTKDEEEMEEAANQKLALQKAKEVAEVSPMSAANISIAAFVKQTRGTVSRSSSVSSVNSPRQQNSAKARSVWEQRASQLRLQNLRASCEALYSEMDPEERLRYASTRHVRPDMKTHMDRPLVVEPGRDGLRGPVGSKSKPEGTEATESADLPRRHHRHRDRDKTSATAPAGGEQDRTESTETGAREERARPRRSHSKETPGADTQVRCERSRRHHRRGSPEEATEREPRRHRAHRHAQDSSKEGTAPVLVPKGERRARHRGPRTGPREAENNEEPTRRHRARHKVPPTLQPPEREAAEKESNAVEGDKETRNHQPKEPHCDLEAIAVTGVGPLHMLPSTCLQKVDEQPEDADNQRNVTRMGSQPSDPSTTVHVPVTLTGPPGETPVVPSGNMNLEGQAEGKKEAEADDVLRRGPRPIVPYSSMFCLSPTNLLRRFCHYIVTMRYFEMVILVVIALSSIALAAEDPVRTDSFRNNALKYMDYIFTGVFTFEMVIKMIDLGLLLHPGAYFRDLWNILDFIVVSGALVAFAFSSFMGGSKGKDINTIKSLRVLRVLRPLKTIKRLPKLKAVFDCVVNSLKNVLNILIVYMLFMFIFAVIAVQLFKGKFFYCTDESKELERDCRGQYLDYEKEEVEAQPRQWKKYDFHYDNVLWALLTLFTVSTGEGWPMVLKHSVDATYEEQGPSPGFRMELSIFYVVYFVVFPFFFVNIFVALIIITFQEQGDKVMSECSLEKNERACIDFAISAKPLTRYMPQNKQSFQYKTWTFVVSPPFEYFIMAMIALNTVVLMMKFYDAPYEYELMLKCLNIVFTSMFSMECILKIIAFGVLNYFRDAWNVFDFVTVLGSITDILVTEIAETNNFINLSFLRLFRAARLIKLLRQGYTIRILLWTFVQSFKALPYVCLLIAMLFFIYAIIGMQVFGNIALDDDTSINRHNNFRTFLQALMLLFRSATGEAWHEIMLSCLGNRACDPHANASECGSDFAYFYFVSFIFLCSFLMLNLFVAVIMDNFEYLTRDSSILGPHHLDEFIRVWAEYDPAACQENLSSDESGVREERMLAKLPPTQPSGPGTCRDHSQDAKPSSRAQTLILGWE

Target ORF information:

RefSeq Version	XM_006497637.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus calcium channel, voltage-dependent, N type, alpha 1B subunit (Cacna1b), transcript variant X15, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006497637.4

ORF Insert Sequence:

ATGGTCCGCT TCGGGGACGA GCTAGGCGGC CGCTATGGGG GCACCGGCGG CGGGGAGCGG 
GCTCGGGGTG GCGGGGCCGG CGGGGCGGGT GGCCCGGGCC AGGGGGGTCT GCCGCCGGGC 
CAGCGGGTCC TGTACAAGCA GTCCATTGCG CAGCGCGCAC GGACTATGGC CCTGTACAAC 
CCCATCCCAG TCAAGCAGAA CTGCTTCACC GTCAACCGCT CGCTCTTCGT CTTCAGCGAG 
GACAACGTCG TCCGCAAATA CGCTAAGCGC ATCACCGAAT GGCCGCCCTT CGAATACATG 
ATCCTGGCCA CCATCATCGC CAACTGCATT GTTCTGGCCC TGGAGCAGCA CCTCCCTGAT 
GGGGACAAGA CTCCCATGTC TGAGCGACTA GATGACACGG AGCCTTACTT CATCGGGATC 
TTTTGCTTCG AGGCGGGCAT CAAGATCATA GCCCTGGGCT TTGTTTTCCA CAAGGGCTCC 
TACCTTCGGA ACGGCTGGAA TGTCATGGAC TTCGTGGTGG TCCTCACGGG GATTCTCGCC 
ACAGCTGGAA CTGACTTTGA CCTGCGCACA CTGAGGGCTG TGCGTGTGCT TAGGCCCCTG 
AAGCTGGTGT CTGGAATTCC AAGCTTGCAG GTGGTGCTTA AGTCCATCAT GAAGGCCATG 
GTCCCGCTGC TGCAGATTGG GCTGCTGCTC TTCTTTGCCA TCCTCATGTT TGCCATCATC 
GGCCTCGAAT TCTATATGGG CAAATTCCAT AAGGCCTGTT TCCCCAACAG CACAGATACA 
GAGCCTGTGG GTGACTTTCC CTGTGGCAAA GATCCCCCTG CTCGTCAGTG TGATGGTGAC 
ACCGAATGCC GGGAGTACTG GCCAGGACCC AACTTTGGCA TCACCAATTT TGACAACATC 
CTGTTTGCCA TCTTGACAGT GTTCCAGTGT ATCACCATGG AGGGCTGGAC TGACATCCTC 
TACAATACAA ATGATGCGGC TGGCAACACA TGGAACTGGT TGTACTTCAT CCCCCTCATC 
ATCATTGGCT CCTTCTTCAT GCTCAACCTG GTGCTGGGTG TGCTTTCCGG AGAGTTTGCC 
AAGGAGCGGG AGCGAGTCGA GAACCGCCGC GCCTTCCTGA AGCTCCGCAG GCAGCAGCAG 
ATTGAGCGAG AGCTGAATGG GTACTTGGAG TGGATCTTCA AGGCAGAGGA AGTCATGTTG 
GCAGAGGAGG ACAAGAATGC AGAAGAGAAA TCCCCTTTGG ATGCAGTGTT GAAGAGAGCT 
GCCACCAAGA AGAGCCGAAA TGACCTCATC CATGCAGAAG AGGGGGAGGA CCGGTTTGTA 
GACCTCTGTG CAGTTGGGTC TCCATTTGCT CGTGCCAGCC TCAAGAGTGG GAAGACGGAG 
AGCTCATCGT ACTTCCGGAG AAAGGAGAAG ATGTTCCGGT TCTTTATCCG GCGTATGGTG 
AAAGCACAGA GCTTCTACTG GGTGGTACTG TGTGTGGTGG CCCTGAACAC ACTGTGTGTG 
GCCATGGTGC ACTATAATCA GCCTCAGCGG CTTACCACTG CACTGTACTT TGCAGAGTTT 
GTTTTCCTGG GTCTCTTCCT CACAGAGATG TCCCTGAAGA TGTATGGCCT AGGGCCCAGA 
AGTTACTTCA GGTCTTCCTT CAACTGCTTT GACTTTGGGG TGATTGTGGG GAGTATCTTT 
GAAGTAGTCT GGGCTGCCAT CAAGCCAGGA ACCTCCTTTG GAATCAGTGT GCTGCGGGCT 
CTGCGACTGC TGAGGATATT CAAAGTTACC AAGTATTGGA ACTCTCTGAG GAACCTGGTG 
GTTTCCCTCC TCAATTCCAT GAAGTCCATC ATCAGCCTTC TCTTCCTGCT TTTCCTCTTC 
ATCGTGGTCT TCGCTCTGTT GGGGATGCAG CTGTTTGGGG GACAGTTCAA CTTTCAAGAT 
GAGACTCCAA CCACCAATTT TGATACCTTC CCAGCTGCCA TCCTCACTGT CTTTCAGATC 
CTGACAGGAG AGGATTGGAA TGCCGTAATG TATCATGGGA TTGAGTCGCA AGGTGGAGTC 
AGCAAAGGCA TGTTTTCTTC CTTTTACTTC ATCGTCCTGA CACTGTTTGG AAACTACACC 
CTGCTGAATG TTTTTCTGGC CATTGCTGTG GACAACCTTG CCAATGCCCA GGAGTTGACC 
AAGGATGAAG AGGAGATGGA AGAAGCAGCC AATCAGAAAC TTGCTCTTCA GAAGGCCAAA 
GAAGTAGCTG AAGTCAGCCC CATGTCTGCT GCCAATATCT CCATCGCTGC TTTTGTAAAG 
CAAACTCGAG GTACTGTATC TCGCAGCTCA TCTGTCTCCA GCGTAAACTC ACCCAGGCAG 
CAGAACTCGG CCAAGGCGCG CTCAGTATGG GAGCAGCGGG CCAGTCAGCT AAGGCTCCAG 
AATCTGCGTG CCAGCTGTGA GGCATTGTAC AGTGAGATGG ACCCTGAGGA GCGCCTGCGT 
TATGCCAGCA CGCGCCATGT GAGGCCAGAC ATGAAGACAC ACATGGACCG ACCCCTAGTG 
GTGGAGCCTG GTCGAGATGG CTTGCGGGGA CCCGTTGGGA GCAAGTCAAA GCCTGAAGGC 
ACGGAGGCCA CAGAAAGCGC GGACCTACCT CGCAGGCACC ACCGGCACCG TGATAGGGAC 
AAGACCTCAG CCACAGCACC TGCTGGAGGC GAACAGGACA GGACAGAAAG CACCGAGACC 
GGGGCCCGGG AGGAACGTGC GCGCCCTCGT CGAAGTCACA GCAAGGAGAC TCCAGGGGCT 
GACACGCAAG TGCGCTGTGA GCGCAGTAGA CGTCACCACC GGCGCGGCTC CCCGGAGGAG 
GCCACTGAAC GGGAGCCTCG GCGCCACCGT GCCCACCGGC ATGCACAGGA CTCAAGCAAG 
GAGGGCACGG CGCCGGTGCT TGTACCCAAG GGTGAGCGAC GAGCAAGACA CCGAGGCCCA 
CGCACGGGTC CACGTGAGGC AGAGAACAAC GAGGAGCCCA CACGCAGGCA CCGTGCAAGG 
CATAAGGTGC CACCCACACT GCAGCCCCCA GAGAGGGAGG CTGCAGAGAA GGAGAGCAAC 
GCGGTGGAAG GGGATAAGGA AACCCGAAAT CACCAGCCCA AGGAACCTCA CTGTGACCTG 
GAGGCCATTG CAGTTACAGG TGTGGGCCCT CTGCACATGC TGCCCAGCAC CTGTCTCCAG 
AAAGTGGACG AGCAACCAGA GGATGCAGAC AACCAGCGTA ATGTCACCCG GATGGGCAGT 
CAGCCCTCAG ATCCCAGCAC CACTGTGCAT GTCCCAGTGA CACTGACAGG CCCTCCTGGG 
GAGACCCCTG TAGTTCCCAG TGGTAACATG AACCTGGAAG GCCAAGCAGA GGGCAAGAAG 
GAGGCAGAGG CGGATGATGT GCTGAGAAGA GGCCCCAGGC CCATCGTTCC CTACAGCTCC 
ATGTTTTGTC TCAGCCCCAC CAACCTGCTT CGTCGCTTCT GCCATTACAT TGTGACCATG 
CGGTACTTTG AGATGGTAAT TCTTGTGGTC ATTGCCTTGA GCAGCATTGC CCTGGCTGCA 
GAGGATCCTG TGCGGACAGA TTCATTCAGG AACAACGCTT TAAAGTACAT GGATTACATC 
TTTACAGGAG TCTTCACCTT TGAAATGGTC ATAAAGATGA TAGACTTGGG CTTGCTGCTG 
CACCCTGGTG CCTACTTCCG GGACCTGTGG AACATTCTGG ACTTCATTGT TGTCAGTGGA 
GCCCTGGTGG CATTTGCGTT CTCGAGCTTC ATGGGAGGAT CCAAAGGGAA AGACATCAAT 
ACCATCAAGT CTCTGAGAGT CCTGCGTGTC CTGAGGCCCC TCAAGACCAT CAAGCGGCTG 
CCTAAGCTCA AGGCTGTCTT TGACTGTGTG GTGAACTCCC TGAAGAACGT CTTGAACATC 
CTGATTGTCT ACATGCTCTT CATGTTCATA TTTGCCGTCA TTGCCGTCCA GCTCTTCAAA 
GGGAAGTTCT TTTACTGTAC TGATGAATCC AAGGAGCTGG AGAGGGACTG CCGGGGTCAG 
TATTTGGATT ATGAGAAGGA AGAAGTAGAA GCCCAGCCAA GGCAGTGGAA GAAATATGAC 
TTCCACTATG ACAATGTTCT CTGGGCCTTG TTGACGCTGT TCACAGTGTC CACGGGAGAG 
GGGTGGCCCA TGGTGCTGAA ACACTCTGTG GATGCCACCT ATGAGGAACA GGGGCCCAGT 
CCCGGGTTCC GGATGGAGCT CTCCATCTTC TACGTGGTCT ACTTTGTGGT CTTCCCTTTT 
TTCTTTGTCA ACATCTTTGT GGCCTTGATC ATTATCACCT TCCAGGAACA GGGAGATAAG 
GTGATGTCTG AATGCAGCTT AGAAAAGAAT GAGAGGGCTT GCATTGATTT TGCCATCAGT 
GCCAAGCCCC TGACACGGTA CATGCCTCAA AACAAACAGT CGTTCCAGTA TAAGACATGG 
ACATTCGTGG TCTCTCCACC CTTTGAGTAC TTCATCATGG CTATGATAGC CCTCAACACA 
GTGGTGCTGA TGATGAAGTT CTATGATGCA CCTTATGAGT ACGAGCTGAT GCTGAAATGC 
CTGAACATTG TCTTCACATC CATGTTCTCG ATGGAGTGCA TACTGAAGAT CATCGCCTTT 
GGGGTATTGA ACTACTTCAG AGATGCCTGG AACGTCTTTG ACTTTGTCAC GGTTTTGGGA 
AGTATTACTG ATATTTTAGT AACAGAGATA GCGGAAACGA ACAACTTCAT CAACCTAAGC 
TTCCTTCGCC TCTTCCGGGC GGCACGGCTG ATCAAGCTGC TTCGCCAGGG CTACACCATC 
CGCATCCTAT TGTGGACCTT CGTCCAGTCC TTTAAGGCGC TGCCCTACGT GTGCCTCCTC 
ATTGCCATGC TGTTCTTCAT CTACGCCATC ATCGGCATGC AGGTTTTTGG AAACATTGCC 
CTTGATGATG ACACCAGTAT CAACCGACAC AACAACTTCC GGACATTTCT GCAAGCCTTA 
ATGCTATTGT TCAGGAGTGC CACTGGGGAG GCCTGGCATG AGATCATGCT GTCTTGTCTG 
GGCAACCGGG CCTGTGACCC ACATGCCAAC GCCAGTGAGT GCGGGAGCGA CTTTGCCTAT 
TTTTATTTTG TCTCCTTCAT CTTCCTCTGT TCCTTTCTGA TGTTGAACCT CTTTGTTGCT 
GTAATCATGG ACAATTTTGA GTACCTCACT CGGGACTCTT CCATCCTAGG GCCTCACCAC 
TTAGACGAAT TCATTCGAGT CTGGGCTGAA TACGACCCAG CTGCGTGCCA GGAGAATCTC 
AGCTCAGATG AGAGTGGGGT CCGGGAGGAG AGAATGCTGG CAAAACTTCC ACCCACCCAG 
CCTTCTGGAC CAGGCACCTG CCGGGACCAC TCTCAGGACG CCAAGCCAAG CTCCAGAGCC 
CAAACACTAA TTCTAGGGTG GGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cacna1b ( XM_006497636.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006497699.1 Mus musculus calcium channel, voltage-dependent, N type, alpha 1B subunit (Cacna1b), transcript variant X14, mRNA.

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CloneID	OMu29267
Clone ID Related Accession (Same CDS sequence)	XM_006497636.4 , XM_006497636.3
Accession Version	XM_006497636.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6672bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	voltage-dependent N-type calcium channel subunit alpha-1B isoform X14
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006497636.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGGATG ACACGGAGCC TTACTTCATC GGGATCTTTT GCTTCGAGGC GGGCATCAAG 
ATCATAGCCC TGGGCTTTGT TTTCCACAAG GGCTCCTACC TTCGGAACGG CTGGAATGTC 
ATGGACTTCG TGGTGGTCCT CACGGGGATT CTCGCCACAG CTGGAACTGA CTTTGACCTG 
CGCACACTGA GGGCTGTGCG TGTGCTTAGG CCCCTGAAGC TGGTGTCTGG AATTCCAAGC 
TTGCAGGTGG TGCTTAAGTC CATCATGAAG GCCATGGTCC CGCTGCTGCA GATTGGGCTG 
CTGCTCTTCT TTGCCATCCT CATGTTTGCC ATCATCGGCC TCGAATTCTA TATGGGCAAA 
TTCCATAAGG CCTGTTTCCC CAACAGCACA GATACAGAGC CTGTGGGTGA CTTTCCCTGT 
GGCAAAGATC CCCCTGCTCG TCAGTGTGAT GGTGACACCG AATGCCGGGA GTACTGGCCA 
GGACCCAACT TTGGCATCAC CAATTTTGAC AACATCCTGT TTGCCATCTT GACAGTGTTC 
CAGTGTATCA CCATGGAGGG CTGGACTGAC ATCCTCTACA ATACAAATGA TGCGGCTGGC 
AACACATGGA ACTGGTTGTA CTTCATCCCC CTCATCATCA TTGGCTCCTT CTTCATGCTC 
AACCTGGTGC TGGGTGTGCT TTCCGGAGAG TTTGCCAAGG AGCGGGAGCG AGTCGAGAAC 
CGCCGCGCCT TCCTGAAGCT CCGCAGGCAG CAGCAGATTG AGCGAGAGCT GAATGGGTAC 
TTGGAGTGGA TCTTCAAGGC AGAGGAAGTC ATGTTGGCAG AGGAGGACAA GAATGCAGAA 
GAGAAATCCC CTTTGGATGC AGTGTTGAAG AGAGCTGCCA CCAAGAAGAG CCGAAATGAC 
CTCATCCATG CAGAAGAGGG GGAGGACCGG TTTGTAGACC TCTGTGCAGT TGGGTCTCCA 
TTTGCTCGTG CCAGCCTCAA GAGTGGGAAG ACGGAGAGCT CATCGTACTT CCGGAGAAAG 
GAGAAGATGT TCCGGTTCTT TATCCGGCGT ATGGTGAAAG CACAGAGCTT CTACTGGGTG 
GTACTGTGTG TGGTGGCCCT GAACACACTG TGTGTGGCCA TGGTGCACTA TAATCAGCCT 
CAGCGGCTTA CCACTGCACT GTACTTTGCA GAGTTTGTTT TCCTGGGTCT CTTCCTCACA 
GAGATGTCCC TGAAGATGTA TGGCCTAGGG CCCAGAAGTT ACTTCAGGTC TTCCTTCAAC 
TGCTTTGACT TTGGGGTGAT TGTGGGGAGT ATCTTTGAAG TAGTCTGGGC TGCCATCAAG 
CCAGGAACCT CCTTTGGAAT CAGTGTGCTG CGGGCTCTGC GACTGCTGAG GATATTCAAA 
GTTACCAAGT ATTGGAACTC TCTGAGGAAC CTGGTGGTTT CCCTCCTCAA TTCCATGAAG 
TCCATCATCA GCCTTCTCTT CCTGCTTTTC CTCTTCATCG TGGTCTTCGC TCTGTTGGGG 
ATGCAGCTGT TTGGGGGACA GTTCAACTTT CAAGATGAGA CTCCAACCAC CAATTTTGAT 
ACCTTCCCAG CTGCCATCCT CACTGTCTTT CAGATCCTGA CAGGAGAGGA TTGGAATGCC 
GTAATGTATC ATGGGATTGA GTCGCAAGGT GGAGTCAGCA AAGGCATGTT TTCTTCCTTT 
TACTTCATCG TCCTGACACT GTTTGGAAAC TACACCCTGC TGAATGTTTT TCTGGCCATT 
GCTGTGGACA ACCTTGCCAA TGCCCAGGAG TTGACCAAGG ATGAAGAGGA GATGGAAGAA 
GCAGCCAATC AGAAACTTGC TCTTCAGAAG GCCAAAGAAG TAGCTGAAGT CAGCCCCATG 
TCTGCTGCCA ATATCTCCAT CGCTGCTTTT GTAAAGCAAA CTCGAGGTAC TGTATCTCGC 
AGCTCATCTG TCTCCAGCGT AAACTCACCC AGGCAGCAGA ACTCGGCCAA GGCGCGCTCA 
GTATGGGAGC AGCGGGCCAG TCAGCTAAGG CTCCAGAATC TGCGTGCCAG CTGTGAGGCA 
TTGTACAGTG AGATGGACCC TGAGGAGCGC CTGCGTTATG CCAGCACGCG CCATGTGAGG 
CCAGACATGA AGACACACAT GGACCGACCC CTAGTGGTGG AGCCTGGTCG AGATGGCTTG 
CGGGGACCCG TTGGGAGCAA GTCAAAGCCT GAAGGCACGG AGGCCACAGA AAGCGCGGAC 
CTACCTCGCA GGCACCACCG GCACCGTGAT AGGGACAAGA CCTCAGCCAC AGCACCTGCT 
GGAGGCGAAC AGGACAGGAC AGAAAGCACC GAGACCGGGG CCCGGGAGGA ACGTGCGCGC 
CCTCGTCGAA GTCACAGCAA GGAGACTCCA GGGGCTGACA CGCAAGTGCG CTGTGAGCGC 
AGTAGACGTC ACCACCGGCG CGGCTCCCCG GAGGAGGCCA CTGAACGGGA GCCTCGGCGC 
CACCGTGCCC ACCGGCATGC ACAGGACTCA AGCAAGGAGG GCACGGCGCC GGTGCTTGTA 
CCCAAGGGTG AGCGACGAGC AAGACACCGA GGCCCACGCA CGGGTCCACG TGAGGCAGAG 
AACAACGAGG AGCCCACACG CAGGCACCGT GCAAGGCATA AGGTGCCACC CACACTGCAG 
CCCCCAGAGA GGGAGGCTGC AGAGAAGGAG AGCAACGCGG TGGAAGGGGA TAAGGAAACC 
CGAAATCACC AGCCCAAGGA ACCTCACTGT GACCTGGAGG CCATTGCAGT TACAGGTGTG 
GGCCCTCTGC ACATGCTGCC CAGCACCTGT CTCCAGAAAG TGGACGAGCA ACCAGAGGAT 
GCAGACAACC AGCGTAATGT CACCCGGATG GGCAGTCAGC CCTCAGATCC CAGCACCACT 
GTGCATGTCC CAGTGACACT GACAGGCCCT CCTGGGGAGA CCCCTGTAGT TCCCAGTGGT 
AACATGAACC TGGAAGGCCA AGCAGAGGGC AAGAAGGAGG CAGAGGCGGA TGATGTGCTG 
AGAAGAGGCC CCAGGCCCAT CGTTCCCTAC AGCTCCATGT TTTGTCTCAG CCCCACCAAC 
CTGCTTCGTC GCTTCTGCCA TTACATTGTG ACCATGCGGT ACTTTGAGAT GGTAATTCTT 
GTGGTCATTG CCTTGAGCAG CATTGCCCTG GCTGCAGAGG ATCCTGTGCG GACAGATTCA 
TTCAGGAACA ACGCTTTAAA GTACATGGAT TACATCTTTA CAGGAGTCTT CACCTTTGAA 
ATGGTCATAA AGATGATAGA CTTGGGCTTG CTGCTGCACC CTGGTGCCTA CTTCCGGGAC 
CTGTGGAACA TTCTGGACTT CATTGTTGTC AGTGGAGCCC TGGTGGCATT TGCGTTCTCG 
AGCTTCATGG GAGGATCCAA AGGGAAAGAC ATCAATACCA TCAAGTCTCT GAGAGTCCTG 
CGTGTCCTGA GGCCCCTCAA GACCATCAAG CGGCTGCCTA AGCTCAAGGC TGTCTTTGAC 
TGTGTGGTGA ACTCCCTGAA GAACGTCTTG AACATCCTGA TTGTCTACAT GCTCTTCATG 
TTCATATTTG CCGTCATTGC CGTCCAGCTC TTCAAAGGGA AGTTCTTTTA CTGTACTGAT 
GAATCCAAGG AGCTGGAGAG GGACTGCCGG GGTCAGTATT TGGATTATGA GAAGGAAGAA 
GTAGAAGCCC AGCCAAGGCA GTGGAAGAAA TATGACTTCC ACTATGACAA TGTTCTCTGG 
GCCTTGTTGA CGCTGTTCAC AGTGTCCACG GGAGAGGGGT GGCCCATGGT GCTGAAACAC 
TCTGTGGATG CCACCTATGA GGAACAGGGG CCCAGTCCCG GGTTCCGGAT GGAGCTCTCC 
ATCTTCTACG TGGTCTACTT TGTGGTCTTC CCTTTTTTCT TTGTCAACAT CTTTGTGGCC 
TTGATCATTA TCACCTTCCA GGAACAGGGA GATAAGGTGA TGTCTGAATG CAGCTTAGAA 
AAGAATGAGA GGGCTTGCAT TGATTTTGCC ATCAGTGCCA AGCCCCTGAC ACGGTACATG 
CCTCAAAACA AACAGTCGTT CCAGTATAAG ACATGGACAT TCGTGGTCTC TCCACCCTTT 
GAGTACTTCA TCATGGCTAT GATAGCCCTC AACACAGTGG TGCTGATGAT GAAGTTCTAT 
GATGCACCTT ATGAGTACGA GCTGATGCTG AAATGCCTGA ACATTGTCTT CACATCCATG 
TTCTCGATGG AGTGCATACT GAAGATCATC GCCTTTGGGG TATTGAACTA CTTCAGAGAT 
GCCTGGAACG TCTTTGACTT TGTCACGGTT TTGGGAAGTA TTACTGATAT TTTAGTAACA 
GAGATAGCGG AAACGAACAA CTTCATCAAC CTAAGCTTCC TTCGCCTCTT CCGGGCGGCA 
CGGCTGATCA AGCTGCTTCG CCAGGGCTAC ACCATCCGCA TCCTATTGTG GACCTTCGTC 
CAGTCCTTTA AGGCGCTGCC CTACGTGTGC CTCCTCATTG CCATGCTGTT CTTCATCTAC 
GCCATCATCG GCATGCAGGT TTTTGGAAAC ATTGCCCTTG ATGATGACAC CAGTATCAAC 
CGACACAACA ACTTCCGGAC ATTTCTGCAA GCCTTAATGC TATTGTTCAG GAGTGCCACT 
GGGGAGGCCT GGCATGAGAT CATGCTGTCT TGTCTGGGCA ACCGGGCCTG TGACCCACAT 
GCCAACGCCA GTGAGTGCGG GAGCGACTTT GCCTATTTTT ATTTTGTCTC CTTCATCTTC 
CTCTGTTCCT TTCTGATGTT GAACCTCTTT GTTGCTGTAA TCATGGACAA TTTTGAGTAC 
CTCACTCGGG ACTCTTCCAT CCTAGGGCCT CACCACTTAG ACGAATTCAT TCGAGTCTGG 
GCTGAATACG ACCCAGCTGC GTGTGGGCGC ATCAGTTACA ATGACATGTT TGAGATGCTG 
AAACACATGT CCCCACCTCT GGGGTTGGGG AAGAAATGCC CGGCTCGAGT TGCATACAAG 
CGCCTGGTTC GCATGAACAT GCCCATATCC AATGAGGACA TGACGGTGCA CTTTACGTCC 
ACACTGATGG CCCTCATCCG GACAGCACTG GAGATCAAGC TTGCCCCAGC GGGGACGAAG 
CAGCACCAGT GTGATGCTGA GCTGAGAAAA GAGATCTCTT CTGTGTGGGC TAATCTGCCC 
CAGAAGACTC TGGACTTACT GGTACCACCC CACAAACCTG ACGAGATGAC AGTGGGGAAG 
GTCTATGCTG CTCTCATGAT ATTTGACTTC TACAAACAGA ACAAAACCAC CAGAGATCAG 
ACTCACCAAG CTCCCGGAGG CCTGTCCCAG ATGGGTCCCG TTTCCCTGTT CCACCCTCTG 
AAGGCCACCC TGGAACAGAC ACAGCCCGCT GTGCTTCGAG GAGCTCGGGT TTTCCTTCGG 
CAAAAGAGTG CAACTTCCCT CAGCAATGGG GGTGCCATAC AAACCCAGGA AAGTGGCATC 
AAGGAGTCGC TGTCCTGGGG CACGCAGAGG ACCCAAGATG CACTTTATGA GGCCAGAGCA 
CCTCTAGAAC GTGGCCATTC TGCAGAGATC CCTGTGGGGC AGTCAGGAAC ACTGGCTGTG 
GATGTCCAGA TGCAGAACAT GACACTGAGA GGACCAGATG GGGAGCCCCA GCCTGGGCTG 
GAAAGCCAAG GCAGAGCTGC CTCTATGCCA CGCCTAGCGG CAGAAACACA GCCGGCCCCT 
AATGCCAGCC CCATGAAGCG CTCCATCTCC ACACTGGCTC CACGCCCACA TGGGACTCAG 
CTTTGCAGCA CAGTTCTGGA CCGGCCTCCT CCTAGCCAGG CATCACATCA CCACCACCAC 
CGCTGCCACC GGCGCAGAGA CAAGAAGCAA AGGTCCCTGG AAAAGGGGCC CAGCCTGTCT 
GTTGACCCAG AAGGTGCACC AAGCACTGCT GCAGGACCTG GTCTGCCCCA TGGAGAAGGA 
TCCACCGCCT GCCGGCGGGA CCGTAAACAG GAGCGAGGCC GGTCCCAGGA GCGGAGGCAG 
CCCTCATCTT CCTCTTCAGA GAAGCAGCGC TTCTATTCCT GTGACCGCTT TGGGAGCCGG 
GAGCCCCCGC AACTGATGCC CTCACTCAGT AGCCACCCCA CATCGCCAAC AGCGGCGCTA 
GAGCCAGCAC CCCACCCACA GGGCAGTGGT TCCGTTAATG GGAGCCCCTT GATGTCAACA 
TCCGGTGCTA GCACCCCGGG CCGAGGTGGG CGGAGGCAGC TCCCCCAGAC TCCTCTGACC 
CCACGCCCCA GCATCACCTA CAAGACCGCC AATTCCTCGC CTGTCCACTT TGCTGAGGGT 
CAGAGCGGCC TCCCAGCCTT CTCCCCTGGC CGTCTCAGCC GCGGCCTTTC TGAACACAAT 
GCCCTGCTCC AGAAAGAGCC CCTGAGCCAG CCTCTAGCTC CTGGCTCCCG AATTGGCTCT 
GACCCTTACC TAGGGCAGCG TCTGGACAGT GAGGCCTCCG CCCACACCCT GCCTGAGGAT 
ACACTCACCT TTGAAGAGGC AGTGGCCACC AACTCTGGCC GCTCCTCCAG GACTTCCTAT 
GTGTCCTCCC TCACTTCCCA ATCCCACCCT CTCCGCCGTG TACCCAATGG CTATCACTGC 
ACTTTGGGAC TCAGCACTGG CGTCCGGGCG CGGCACAGCT ACCACCACCC CGATCAGGAC 
CACTGGTGCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006497699.1
CDS	338..7009
Translation	MQDDTEPYFIGIFCFEAGIKIIALGFVFHKGSYLRNGWNVMDFVVVLTGILATAGTDFDLRTLRAVRVLRPLKLVSGIPSLQVVLKSIMKAMVPLLQIGLLLFFAILMFAIIGLEFYMGKFHKACFPNSTDTEPVGDFPCGKDPPARQCDGDTECREYWPGPNFGITNFDNILFAILTVFQCITMEGWTDILYNTNDAAGNTWNWLYFIPLIIIGSFFMLNLVLGVLSGEFAKERERVENRRAFLKLRRQQQIERELNGYLEWIFKAEEVMLAEEDKNAEEKSPLDAVLKRAATKKSRNDLIHAEEGEDRFVDLCAVGSPFARASLKSGKTESSSYFRRKEKMFRFFIRRMVKAQSFYWVVLCVVALNTLCVAMVHYNQPQRLTTALYFAEFVFLGLFLTEMSLKMYGLGPRSYFRSSFNCFDFGVIVGSIFEVVWAAIKPGTSFGISVLRALRLLRIFKVTKYWNSLRNLVVSLLNSMKSIISLLFLLFLFIVVFALLGMQLFGGQFNFQDETPTTNFDTFPAAILTVFQILTGEDWNAVMYHGIESQGGVSKGMFSSFYFIVLTLFGNYTLLNVFLAIAVDNLANAQELTKDEEEMEEAANQKLALQKAKEVAEVSPMSAANISIAAFVKQTRGTVSRSSSVSSVNSPRQQNSAKARSVWEQRASQLRLQNLRASCEALYSEMDPEERLRYASTRHVRPDMKTHMDRPLVVEPGRDGLRGPVGSKSKPEGTEATESADLPRRHHRHRDRDKTSATAPAGGEQDRTESTETGAREERARPRRSHSKETPGADTQVRCERSRRHHRRGSPEEATEREPRRHRAHRHAQDSSKEGTAPVLVPKGERRARHRGPRTGPREAENNEEPTRRHRARHKVPPTLQPPEREAAEKESNAVEGDKETRNHQPKEPHCDLEAIAVTGVGPLHMLPSTCLQKVDEQPEDADNQRNVTRMGSQPSDPSTTVHVPVTLTGPPGETPVVPSGNMNLEGQAEGKKEAEADDVLRRGPRPIVPYSSMFCLSPTNLLRRFCHYIVTMRYFEMVILVVIALSSIALAAEDPVRTDSFRNNALKYMDYIFTGVFTFEMVIKMIDLGLLLHPGAYFRDLWNILDFIVVSGALVAFAFSSFMGGSKGKDINTIKSLRVLRVLRPLKTIKRLPKLKAVFDCVVNSLKNVLNILIVYMLFMFIFAVIAVQLFKGKFFYCTDESKELERDCRGQYLDYEKEEVEAQPRQWKKYDFHYDNVLWALLTLFTVSTGEGWPMVLKHSVDATYEEQGPSPGFRMELSIFYVVYFVVFPFFFVNIFVALIIITFQEQGDKVMSECSLEKNERACIDFAISAKPLTRYMPQNKQSFQYKTWTFVVSPPFEYFIMAMIALNTVVLMMKFYDAPYEYELMLKCLNIVFTSMFSMECILKIIAFGVLNYFRDAWNVFDFVTVLGSITDILVTEIAETNNFINLSFLRLFRAARLIKLLRQGYTIRILLWTFVQSFKALPYVCLLIAMLFFIYAIIGMQVFGNIALDDDTSINRHNNFRTFLQALMLLFRSATGEAWHEIMLSCLGNRACDPHANASECGSDFAYFYFVSFIFLCSFLMLNLFVAVIMDNFEYLTRDSSILGPHHLDEFIRVWAEYDPAACGRISYNDMFEMLKHMSPPLGLGKKCPARVAYKRLVRMNMPISNEDMTVHFTSTLMALIRTALEIKLAPAGTKQHQCDAELRKEISSVWANLPQKTLDLLVPPHKPDEMTVGKVYAALMIFDFYKQNKTTRDQTHQAPGGLSQMGPVSLFHPLKATLEQTQPAVLRGARVFLRQKSATSLSNGGAIQTQESGIKESLSWGTQRTQDALYEARAPLERGHSAEIPVGQSGTLAVDVQMQNMTLRGPDGEPQPGLESQGRAASMPRLAAETQPAPNASPMKRSISTLAPRPHGTQLCSTVLDRPPPSQASHHHHHRCHRRRDKKQRSLEKGPSLSVDPEGAPSTAAGPGLPHGEGSTACRRDRKQERGRSQERRQPSSSSSEKQRFYSCDRFGSREPPQLMPSLSSHPTSPTAALEPAPHPQGSGSVNGSPLMSTSGASTPGRGGRRQLPQTPLTPRPSITYKTANSSPVHFAEGQSGLPAFSPGRLSRGLSEHNALLQKEPLSQPLAPGSRIGSDPYLGQRLDSEASAHTLPEDTLTFEEAVATNSGRSSRTSYVSSLTSQSHPLRRVPNGYHCTLGLSTGVRARHSYHHPDQDHWC

Target ORF information:

RefSeq Version	XM_006497636.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus calcium channel, voltage-dependent, N type, alpha 1B subunit (Cacna1b), transcript variant X14, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006497636.4

ORF Insert Sequence:

ATGCAGGATG ACACGGAGCC TTACTTCATC GGGATCTTTT GCTTCGAGGC GGGCATCAAG 
ATCATAGCCC TGGGCTTTGT TTTCCACAAG GGCTCCTACC TTCGGAACGG CTGGAATGTC 
ATGGACTTCG TGGTGGTCCT CACGGGGATT CTCGCCACAG CTGGAACTGA CTTTGACCTG 
CGCACACTGA GGGCTGTGCG TGTGCTTAGG CCCCTGAAGC TGGTGTCTGG AATTCCAAGC 
TTGCAGGTGG TGCTTAAGTC CATCATGAAG GCCATGGTCC CGCTGCTGCA GATTGGGCTG 
CTGCTCTTCT TTGCCATCCT CATGTTTGCC ATCATCGGCC TCGAATTCTA TATGGGCAAA 
TTCCATAAGG CCTGTTTCCC CAACAGCACA GATACAGAGC CTGTGGGTGA CTTTCCCTGT 
GGCAAAGATC CCCCTGCTCG TCAGTGTGAT GGTGACACCG AATGCCGGGA GTACTGGCCA 
GGACCCAACT TTGGCATCAC CAATTTTGAC AACATCCTGT TTGCCATCTT GACAGTGTTC 
CAGTGTATCA CCATGGAGGG CTGGACTGAC ATCCTCTACA ATACAAATGA TGCGGCTGGC 
AACACATGGA ACTGGTTGTA CTTCATCCCC CTCATCATCA TTGGCTCCTT CTTCATGCTC 
AACCTGGTGC TGGGTGTGCT TTCCGGAGAG TTTGCCAAGG AGCGGGAGCG AGTCGAGAAC 
CGCCGCGCCT TCCTGAAGCT CCGCAGGCAG CAGCAGATTG AGCGAGAGCT GAATGGGTAC 
TTGGAGTGGA TCTTCAAGGC AGAGGAAGTC ATGTTGGCAG AGGAGGACAA GAATGCAGAA 
GAGAAATCCC CTTTGGATGC AGTGTTGAAG AGAGCTGCCA CCAAGAAGAG CCGAAATGAC 
CTCATCCATG CAGAAGAGGG GGAGGACCGG TTTGTAGACC TCTGTGCAGT TGGGTCTCCA 
TTTGCTCGTG CCAGCCTCAA GAGTGGGAAG ACGGAGAGCT CATCGTACTT CCGGAGAAAG 
GAGAAGATGT TCCGGTTCTT TATCCGGCGT ATGGTGAAAG CACAGAGCTT CTACTGGGTG 
GTACTGTGTG TGGTGGCCCT GAACACACTG TGTGTGGCCA TGGTGCACTA TAATCAGCCT 
CAGCGGCTTA CCACTGCACT GTACTTTGCA GAGTTTGTTT TCCTGGGTCT CTTCCTCACA 
GAGATGTCCC TGAAGATGTA TGGCCTAGGG CCCAGAAGTT ACTTCAGGTC TTCCTTCAAC 
TGCTTTGACT TTGGGGTGAT TGTGGGGAGT ATCTTTGAAG TAGTCTGGGC TGCCATCAAG 
CCAGGAACCT CCTTTGGAAT CAGTGTGCTG CGGGCTCTGC GACTGCTGAG GATATTCAAA 
GTTACCAAGT ATTGGAACTC TCTGAGGAAC CTGGTGGTTT CCCTCCTCAA TTCCATGAAG 
TCCATCATCA GCCTTCTCTT CCTGCTTTTC CTCTTCATCG TGGTCTTCGC TCTGTTGGGG 
ATGCAGCTGT TTGGGGGACA GTTCAACTTT CAAGATGAGA CTCCAACCAC CAATTTTGAT 
ACCTTCCCAG CTGCCATCCT CACTGTCTTT CAGATCCTGA CAGGAGAGGA TTGGAATGCC 
GTAATGTATC ATGGGATTGA GTCGCAAGGT GGAGTCAGCA AAGGCATGTT TTCTTCCTTT 
TACTTCATCG TCCTGACACT GTTTGGAAAC TACACCCTGC TGAATGTTTT TCTGGCCATT 
GCTGTGGACA ACCTTGCCAA TGCCCAGGAG TTGACCAAGG ATGAAGAGGA GATGGAAGAA 
GCAGCCAATC AGAAACTTGC TCTTCAGAAG GCCAAAGAAG TAGCTGAAGT CAGCCCCATG 
TCTGCTGCCA ATATCTCCAT CGCTGCTTTT GTAAAGCAAA CTCGAGGTAC TGTATCTCGC 
AGCTCATCTG TCTCCAGCGT AAACTCACCC AGGCAGCAGA ACTCGGCCAA GGCGCGCTCA 
GTATGGGAGC AGCGGGCCAG TCAGCTAAGG CTCCAGAATC TGCGTGCCAG CTGTGAGGCA 
TTGTACAGTG AGATGGACCC TGAGGAGCGC CTGCGTTATG CCAGCACGCG CCATGTGAGG 
CCAGACATGA AGACACACAT GGACCGACCC CTAGTGGTGG AGCCTGGTCG AGATGGCTTG 
CGGGGACCCG TTGGGAGCAA GTCAAAGCCT GAAGGCACGG AGGCCACAGA AAGCGCGGAC 
CTACCTCGCA GGCACCACCG GCACCGTGAT AGGGACAAGA CCTCAGCCAC AGCACCTGCT 
GGAGGCGAAC AGGACAGGAC AGAAAGCACC GAGACCGGGG CCCGGGAGGA ACGTGCGCGC 
CCTCGTCGAA GTCACAGCAA GGAGACTCCA GGGGCTGACA CGCAAGTGCG CTGTGAGCGC 
AGTAGACGTC ACCACCGGCG CGGCTCCCCG GAGGAGGCCA CTGAACGGGA GCCTCGGCGC 
CACCGTGCCC ACCGGCATGC ACAGGACTCA AGCAAGGAGG GCACGGCGCC GGTGCTTGTA 
CCCAAGGGTG AGCGACGAGC AAGACACCGA GGCCCACGCA CGGGTCCACG TGAGGCAGAG 
AACAACGAGG AGCCCACACG CAGGCACCGT GCAAGGCATA AGGTGCCACC CACACTGCAG 
CCCCCAGAGA GGGAGGCTGC AGAGAAGGAG AGCAACGCGG TGGAAGGGGA TAAGGAAACC 
CGAAATCACC AGCCCAAGGA ACCTCACTGT GACCTGGAGG CCATTGCAGT TACAGGTGTG 
GGCCCTCTGC ACATGCTGCC CAGCACCTGT CTCCAGAAAG TGGACGAGCA ACCAGAGGAT 
GCAGACAACC AGCGTAATGT CACCCGGATG GGCAGTCAGC CCTCAGATCC CAGCACCACT 
GTGCATGTCC CAGTGACACT GACAGGCCCT CCTGGGGAGA CCCCTGTAGT TCCCAGTGGT 
AACATGAACC TGGAAGGCCA AGCAGAGGGC AAGAAGGAGG CAGAGGCGGA TGATGTGCTG 
AGAAGAGGCC CCAGGCCCAT CGTTCCCTAC AGCTCCATGT TTTGTCTCAG CCCCACCAAC 
CTGCTTCGTC GCTTCTGCCA TTACATTGTG ACCATGCGGT ACTTTGAGAT GGTAATTCTT 
GTGGTCATTG CCTTGAGCAG CATTGCCCTG GCTGCAGAGG ATCCTGTGCG GACAGATTCA 
TTCAGGAACA ACGCTTTAAA GTACATGGAT TACATCTTTA CAGGAGTCTT CACCTTTGAA 
ATGGTCATAA AGATGATAGA CTTGGGCTTG CTGCTGCACC CTGGTGCCTA CTTCCGGGAC 
CTGTGGAACA TTCTGGACTT CATTGTTGTC AGTGGAGCCC TGGTGGCATT TGCGTTCTCG 
AGCTTCATGG GAGGATCCAA AGGGAAAGAC ATCAATACCA TCAAGTCTCT GAGAGTCCTG 
CGTGTCCTGA GGCCCCTCAA GACCATCAAG CGGCTGCCTA AGCTCAAGGC TGTCTTTGAC 
TGTGTGGTGA ACTCCCTGAA GAACGTCTTG AACATCCTGA TTGTCTACAT GCTCTTCATG 
TTCATATTTG CCGTCATTGC CGTCCAGCTC TTCAAAGGGA AGTTCTTTTA CTGTACTGAT 
GAATCCAAGG AGCTGGAGAG GGACTGCCGG GGTCAGTATT TGGATTATGA GAAGGAAGAA 
GTAGAAGCCC AGCCAAGGCA GTGGAAGAAA TATGACTTCC ACTATGACAA TGTTCTCTGG 
GCCTTGTTGA CGCTGTTCAC AGTGTCCACG GGAGAGGGGT GGCCCATGGT GCTGAAACAC 
TCTGTGGATG CCACCTATGA GGAACAGGGG CCCAGTCCCG GGTTCCGGAT GGAGCTCTCC 
ATCTTCTACG TGGTCTACTT TGTGGTCTTC CCTTTTTTCT TTGTCAACAT CTTTGTGGCC 
TTGATCATTA TCACCTTCCA GGAACAGGGA GATAAGGTGA TGTCTGAATG CAGCTTAGAA 
AAGAATGAGA GGGCTTGCAT TGATTTTGCC ATCAGTGCCA AGCCCCTGAC ACGGTACATG 
CCTCAAAACA AACAGTCGTT CCAGTATAAG ACATGGACAT TCGTGGTCTC TCCACCCTTT 
GAGTACTTCA TCATGGCTAT GATAGCCCTC AACACAGTGG TGCTGATGAT GAAGTTCTAT 
GATGCACCTT ATGAGTACGA GCTGATGCTG AAATGCCTGA ACATTGTCTT CACATCCATG 
TTCTCGATGG AGTGCATACT GAAGATCATC GCCTTTGGGG TATTGAACTA CTTCAGAGAT 
GCCTGGAACG TCTTTGACTT TGTCACGGTT TTGGGAAGTA TTACTGATAT TTTAGTAACA 
GAGATAGCGG AAACGAACAA CTTCATCAAC CTAAGCTTCC TTCGCCTCTT CCGGGCGGCA 
CGGCTGATCA AGCTGCTTCG CCAGGGCTAC ACCATCCGCA TCCTATTGTG GACCTTCGTC 
CAGTCCTTTA AGGCGCTGCC CTACGTGTGC CTCCTCATTG CCATGCTGTT CTTCATCTAC 
GCCATCATCG GCATGCAGGT TTTTGGAAAC ATTGCCCTTG ATGATGACAC CAGTATCAAC 
CGACACAACA ACTTCCGGAC ATTTCTGCAA GCCTTAATGC TATTGTTCAG GAGTGCCACT 
GGGGAGGCCT GGCATGAGAT CATGCTGTCT TGTCTGGGCA ACCGGGCCTG TGACCCACAT 
GCCAACGCCA GTGAGTGCGG GAGCGACTTT GCCTATTTTT ATTTTGTCTC CTTCATCTTC 
CTCTGTTCCT TTCTGATGTT GAACCTCTTT GTTGCTGTAA TCATGGACAA TTTTGAGTAC 
CTCACTCGGG ACTCTTCCAT CCTAGGGCCT CACCACTTAG ACGAATTCAT TCGAGTCTGG 
GCTGAATACG ACCCAGCTGC GTGTGGGCGC ATCAGTTACA ATGACATGTT TGAGATGCTG 
AAACACATGT CCCCACCTCT GGGGTTGGGG AAGAAATGCC CGGCTCGAGT TGCATACAAG 
CGCCTGGTTC GCATGAACAT GCCCATATCC AATGAGGACA TGACGGTGCA CTTTACGTCC 
ACACTGATGG CCCTCATCCG GACAGCACTG GAGATCAAGC TTGCCCCAGC GGGGACGAAG 
CAGCACCAGT GTGATGCTGA GCTGAGAAAA GAGATCTCTT CTGTGTGGGC TAATCTGCCC 
CAGAAGACTC TGGACTTACT GGTACCACCC CACAAACCTG ACGAGATGAC AGTGGGGAAG 
GTCTATGCTG CTCTCATGAT ATTTGACTTC TACAAACAGA ACAAAACCAC CAGAGATCAG 
ACTCACCAAG CTCCCGGAGG CCTGTCCCAG ATGGGTCCCG TTTCCCTGTT CCACCCTCTG 
AAGGCCACCC TGGAACAGAC ACAGCCCGCT GTGCTTCGAG GAGCTCGGGT TTTCCTTCGG 
CAAAAGAGTG CAACTTCCCT CAGCAATGGG GGTGCCATAC AAACCCAGGA AAGTGGCATC 
AAGGAGTCGC TGTCCTGGGG CACGCAGAGG ACCCAAGATG CACTTTATGA GGCCAGAGCA 
CCTCTAGAAC GTGGCCATTC TGCAGAGATC CCTGTGGGGC AGTCAGGAAC ACTGGCTGTG 
GATGTCCAGA TGCAGAACAT GACACTGAGA GGACCAGATG GGGAGCCCCA GCCTGGGCTG 
GAAAGCCAAG GCAGAGCTGC CTCTATGCCA CGCCTAGCGG CAGAAACACA GCCGGCCCCT 
AATGCCAGCC CCATGAAGCG CTCCATCTCC ACACTGGCTC CACGCCCACA TGGGACTCAG 
CTTTGCAGCA CAGTTCTGGA CCGGCCTCCT CCTAGCCAGG CATCACATCA CCACCACCAC 
CGCTGCCACC GGCGCAGAGA CAAGAAGCAA AGGTCCCTGG AAAAGGGGCC CAGCCTGTCT 
GTTGACCCAG AAGGTGCACC AAGCACTGCT GCAGGACCTG GTCTGCCCCA TGGAGAAGGA 
TCCACCGCCT GCCGGCGGGA CCGTAAACAG GAGCGAGGCC GGTCCCAGGA GCGGAGGCAG 
CCCTCATCTT CCTCTTCAGA GAAGCAGCGC TTCTATTCCT GTGACCGCTT TGGGAGCCGG 
GAGCCCCCGC AACTGATGCC CTCACTCAGT AGCCACCCCA CATCGCCAAC AGCGGCGCTA 
GAGCCAGCAC CCCACCCACA GGGCAGTGGT TCCGTTAATG GGAGCCCCTT GATGTCAACA 
TCCGGTGCTA GCACCCCGGG CCGAGGTGGG CGGAGGCAGC TCCCCCAGAC TCCTCTGACC 
CCACGCCCCA GCATCACCTA CAAGACCGCC AATTCCTCGC CTGTCCACTT TGCTGAGGGT 
CAGAGCGGCC TCCCAGCCTT CTCCCCTGGC CGTCTCAGCC GCGGCCTTTC TGAACACAAT 
GCCCTGCTCC AGAAAGAGCC CCTGAGCCAG CCTCTAGCTC CTGGCTCCCG AATTGGCTCT 
GACCCTTACC TAGGGCAGCG TCTGGACAGT GAGGCCTCCG CCCACACCCT GCCTGAGGAT 
ACACTCACCT TTGAAGAGGC AGTGGCCACC AACTCTGGCC GCTCCTCCAG GACTTCCTAT 
GTGTCCTCCC TCACTTCCCA ATCCCACCCT CTCCGCCGTG TACCCAATGG CTATCACTGC 
ACTTTGGGAC TCAGCACTGG CGTCCGGGCG CGGCACAGCT ACCACCACCC CGATCAGGAC 
CACTGGTGCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cacna1b ( XM_006497636.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006497699.1 Mus musculus calcium channel, voltage-dependent, N type, alpha 1B subunit (Cacna1b), transcript variant X14, mRNA.

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CloneID	OMu29267
Clone ID Related Accession (Same CDS sequence)	XM_006497636.4 , XM_006497636.3
Accession Version	XM_006497636.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6672bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	voltage-dependent N-type calcium channel subunit alpha-1B isoform X14
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006497636.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGGATG ACACGGAGCC TTACTTCATC GGGATCTTTT GCTTCGAGGC GGGCATCAAG 
ATCATAGCCC TGGGCTTTGT TTTCCACAAG GGCTCCTACC TTCGGAACGG CTGGAATGTC 
ATGGACTTCG TGGTGGTCCT CACGGGGATT CTCGCCACAG CTGGAACTGA CTTTGACCTG 
CGCACACTGA GGGCTGTGCG TGTGCTTAGG CCCCTGAAGC TGGTGTCTGG AATTCCAAGC 
TTGCAGGTGG TGCTTAAGTC CATCATGAAG GCCATGGTCC CGCTGCTGCA GATTGGGCTG 
CTGCTCTTCT TTGCCATCCT CATGTTTGCC ATCATCGGCC TCGAATTCTA TATGGGCAAA 
TTCCATAAGG CCTGTTTCCC CAACAGCACA GATACAGAGC CTGTGGGTGA CTTTCCCTGT 
GGCAAAGATC CCCCTGCTCG TCAGTGTGAT GGTGACACCG AATGCCGGGA GTACTGGCCA 
GGACCCAACT TTGGCATCAC CAATTTTGAC AACATCCTGT TTGCCATCTT GACAGTGTTC 
CAGTGTATCA CCATGGAGGG CTGGACTGAC ATCCTCTACA ATACAAATGA TGCGGCTGGC 
AACACATGGA ACTGGTTGTA CTTCATCCCC CTCATCATCA TTGGCTCCTT CTTCATGCTC 
AACCTGGTGC TGGGTGTGCT TTCCGGAGAG TTTGCCAAGG AGCGGGAGCG AGTCGAGAAC 
CGCCGCGCCT TCCTGAAGCT CCGCAGGCAG CAGCAGATTG AGCGAGAGCT GAATGGGTAC 
TTGGAGTGGA TCTTCAAGGC AGAGGAAGTC ATGTTGGCAG AGGAGGACAA GAATGCAGAA 
GAGAAATCCC CTTTGGATGC AGTGTTGAAG AGAGCTGCCA CCAAGAAGAG CCGAAATGAC 
CTCATCCATG CAGAAGAGGG GGAGGACCGG TTTGTAGACC TCTGTGCAGT TGGGTCTCCA 
TTTGCTCGTG CCAGCCTCAA GAGTGGGAAG ACGGAGAGCT CATCGTACTT CCGGAGAAAG 
GAGAAGATGT TCCGGTTCTT TATCCGGCGT ATGGTGAAAG CACAGAGCTT CTACTGGGTG 
GTACTGTGTG TGGTGGCCCT GAACACACTG TGTGTGGCCA TGGTGCACTA TAATCAGCCT 
CAGCGGCTTA CCACTGCACT GTACTTTGCA GAGTTTGTTT TCCTGGGTCT CTTCCTCACA 
GAGATGTCCC TGAAGATGTA TGGCCTAGGG CCCAGAAGTT ACTTCAGGTC TTCCTTCAAC 
TGCTTTGACT TTGGGGTGAT TGTGGGGAGT ATCTTTGAAG TAGTCTGGGC TGCCATCAAG 
CCAGGAACCT CCTTTGGAAT CAGTGTGCTG CGGGCTCTGC GACTGCTGAG GATATTCAAA 
GTTACCAAGT ATTGGAACTC TCTGAGGAAC CTGGTGGTTT CCCTCCTCAA TTCCATGAAG 
TCCATCATCA GCCTTCTCTT CCTGCTTTTC CTCTTCATCG TGGTCTTCGC TCTGTTGGGG 
ATGCAGCTGT TTGGGGGACA GTTCAACTTT CAAGATGAGA CTCCAACCAC CAATTTTGAT 
ACCTTCCCAG CTGCCATCCT CACTGTCTTT CAGATCCTGA CAGGAGAGGA TTGGAATGCC 
GTAATGTATC ATGGGATTGA GTCGCAAGGT GGAGTCAGCA AAGGCATGTT TTCTTCCTTT 
TACTTCATCG TCCTGACACT GTTTGGAAAC TACACCCTGC TGAATGTTTT TCTGGCCATT 
GCTGTGGACA ACCTTGCCAA TGCCCAGGAG TTGACCAAGG ATGAAGAGGA GATGGAAGAA 
GCAGCCAATC AGAAACTTGC TCTTCAGAAG GCCAAAGAAG TAGCTGAAGT CAGCCCCATG 
TCTGCTGCCA ATATCTCCAT CGCTGCTTTT GTAAAGCAAA CTCGAGGTAC TGTATCTCGC 
AGCTCATCTG TCTCCAGCGT AAACTCACCC AGGCAGCAGA ACTCGGCCAA GGCGCGCTCA 
GTATGGGAGC AGCGGGCCAG TCAGCTAAGG CTCCAGAATC TGCGTGCCAG CTGTGAGGCA 
TTGTACAGTG AGATGGACCC TGAGGAGCGC CTGCGTTATG CCAGCACGCG CCATGTGAGG 
CCAGACATGA AGACACACAT GGACCGACCC CTAGTGGTGG AGCCTGGTCG AGATGGCTTG 
CGGGGACCCG TTGGGAGCAA GTCAAAGCCT GAAGGCACGG AGGCCACAGA AAGCGCGGAC 
CTACCTCGCA GGCACCACCG GCACCGTGAT AGGGACAAGA CCTCAGCCAC AGCACCTGCT 
GGAGGCGAAC AGGACAGGAC AGAAAGCACC GAGACCGGGG CCCGGGAGGA ACGTGCGCGC 
CCTCGTCGAA GTCACAGCAA GGAGACTCCA GGGGCTGACA CGCAAGTGCG CTGTGAGCGC 
AGTAGACGTC ACCACCGGCG CGGCTCCCCG GAGGAGGCCA CTGAACGGGA GCCTCGGCGC 
CACCGTGCCC ACCGGCATGC ACAGGACTCA AGCAAGGAGG GCACGGCGCC GGTGCTTGTA 
CCCAAGGGTG AGCGACGAGC AAGACACCGA GGCCCACGCA CGGGTCCACG TGAGGCAGAG 
AACAACGAGG AGCCCACACG CAGGCACCGT GCAAGGCATA AGGTGCCACC CACACTGCAG 
CCCCCAGAGA GGGAGGCTGC AGAGAAGGAG AGCAACGCGG TGGAAGGGGA TAAGGAAACC 
CGAAATCACC AGCCCAAGGA ACCTCACTGT GACCTGGAGG CCATTGCAGT TACAGGTGTG 
GGCCCTCTGC ACATGCTGCC CAGCACCTGT CTCCAGAAAG TGGACGAGCA ACCAGAGGAT 
GCAGACAACC AGCGTAATGT CACCCGGATG GGCAGTCAGC CCTCAGATCC CAGCACCACT 
GTGCATGTCC CAGTGACACT GACAGGCCCT CCTGGGGAGA CCCCTGTAGT TCCCAGTGGT 
AACATGAACC TGGAAGGCCA AGCAGAGGGC AAGAAGGAGG CAGAGGCGGA TGATGTGCTG 
AGAAGAGGCC CCAGGCCCAT CGTTCCCTAC AGCTCCATGT TTTGTCTCAG CCCCACCAAC 
CTGCTTCGTC GCTTCTGCCA TTACATTGTG ACCATGCGGT ACTTTGAGAT GGTAATTCTT 
GTGGTCATTG CCTTGAGCAG CATTGCCCTG GCTGCAGAGG ATCCTGTGCG GACAGATTCA 
TTCAGGAACA ACGCTTTAAA GTACATGGAT TACATCTTTA CAGGAGTCTT CACCTTTGAA 
ATGGTCATAA AGATGATAGA CTTGGGCTTG CTGCTGCACC CTGGTGCCTA CTTCCGGGAC 
CTGTGGAACA TTCTGGACTT CATTGTTGTC AGTGGAGCCC TGGTGGCATT TGCGTTCTCG 
AGCTTCATGG GAGGATCCAA AGGGAAAGAC ATCAATACCA TCAAGTCTCT GAGAGTCCTG 
CGTGTCCTGA GGCCCCTCAA GACCATCAAG CGGCTGCCTA AGCTCAAGGC TGTCTTTGAC 
TGTGTGGTGA ACTCCCTGAA GAACGTCTTG AACATCCTGA TTGTCTACAT GCTCTTCATG 
TTCATATTTG CCGTCATTGC CGTCCAGCTC TTCAAAGGGA AGTTCTTTTA CTGTACTGAT 
GAATCCAAGG AGCTGGAGAG GGACTGCCGG GGTCAGTATT TGGATTATGA GAAGGAAGAA 
GTAGAAGCCC AGCCAAGGCA GTGGAAGAAA TATGACTTCC ACTATGACAA TGTTCTCTGG 
GCCTTGTTGA CGCTGTTCAC AGTGTCCACG GGAGAGGGGT GGCCCATGGT GCTGAAACAC 
TCTGTGGATG CCACCTATGA GGAACAGGGG CCCAGTCCCG GGTTCCGGAT GGAGCTCTCC 
ATCTTCTACG TGGTCTACTT TGTGGTCTTC CCTTTTTTCT TTGTCAACAT CTTTGTGGCC 
TTGATCATTA TCACCTTCCA GGAACAGGGA GATAAGGTGA TGTCTGAATG CAGCTTAGAA 
AAGAATGAGA GGGCTTGCAT TGATTTTGCC ATCAGTGCCA AGCCCCTGAC ACGGTACATG 
CCTCAAAACA AACAGTCGTT CCAGTATAAG ACATGGACAT TCGTGGTCTC TCCACCCTTT 
GAGTACTTCA TCATGGCTAT GATAGCCCTC AACACAGTGG TGCTGATGAT GAAGTTCTAT 
GATGCACCTT ATGAGTACGA GCTGATGCTG AAATGCCTGA ACATTGTCTT CACATCCATG 
TTCTCGATGG AGTGCATACT GAAGATCATC GCCTTTGGGG TATTGAACTA CTTCAGAGAT 
GCCTGGAACG TCTTTGACTT TGTCACGGTT TTGGGAAGTA TTACTGATAT TTTAGTAACA 
GAGATAGCGG AAACGAACAA CTTCATCAAC CTAAGCTTCC TTCGCCTCTT CCGGGCGGCA 
CGGCTGATCA AGCTGCTTCG CCAGGGCTAC ACCATCCGCA TCCTATTGTG GACCTTCGTC 
CAGTCCTTTA AGGCGCTGCC CTACGTGTGC CTCCTCATTG CCATGCTGTT CTTCATCTAC 
GCCATCATCG GCATGCAGGT TTTTGGAAAC ATTGCCCTTG ATGATGACAC CAGTATCAAC 
CGACACAACA ACTTCCGGAC ATTTCTGCAA GCCTTAATGC TATTGTTCAG GAGTGCCACT 
GGGGAGGCCT GGCATGAGAT CATGCTGTCT TGTCTGGGCA ACCGGGCCTG TGACCCACAT 
GCCAACGCCA GTGAGTGCGG GAGCGACTTT GCCTATTTTT ATTTTGTCTC CTTCATCTTC 
CTCTGTTCCT TTCTGATGTT GAACCTCTTT GTTGCTGTAA TCATGGACAA TTTTGAGTAC 
CTCACTCGGG ACTCTTCCAT CCTAGGGCCT CACCACTTAG ACGAATTCAT TCGAGTCTGG 
GCTGAATACG ACCCAGCTGC GTGTGGGCGC ATCAGTTACA ATGACATGTT TGAGATGCTG 
AAACACATGT CCCCACCTCT GGGGTTGGGG AAGAAATGCC CGGCTCGAGT TGCATACAAG 
CGCCTGGTTC GCATGAACAT GCCCATATCC AATGAGGACA TGACGGTGCA CTTTACGTCC 
ACACTGATGG CCCTCATCCG GACAGCACTG GAGATCAAGC TTGCCCCAGC GGGGACGAAG 
CAGCACCAGT GTGATGCTGA GCTGAGAAAA GAGATCTCTT CTGTGTGGGC TAATCTGCCC 
CAGAAGACTC TGGACTTACT GGTACCACCC CACAAACCTG ACGAGATGAC AGTGGGGAAG 
GTCTATGCTG CTCTCATGAT ATTTGACTTC TACAAACAGA ACAAAACCAC CAGAGATCAG 
ACTCACCAAG CTCCCGGAGG CCTGTCCCAG ATGGGTCCCG TTTCCCTGTT CCACCCTCTG 
AAGGCCACCC TGGAACAGAC ACAGCCCGCT GTGCTTCGAG GAGCTCGGGT TTTCCTTCGG 
CAAAAGAGTG CAACTTCCCT CAGCAATGGG GGTGCCATAC AAACCCAGGA AAGTGGCATC 
AAGGAGTCGC TGTCCTGGGG CACGCAGAGG ACCCAAGATG CACTTTATGA GGCCAGAGCA 
CCTCTAGAAC GTGGCCATTC TGCAGAGATC CCTGTGGGGC AGTCAGGAAC ACTGGCTGTG 
GATGTCCAGA TGCAGAACAT GACACTGAGA GGACCAGATG GGGAGCCCCA GCCTGGGCTG 
GAAAGCCAAG GCAGAGCTGC CTCTATGCCA CGCCTAGCGG CAGAAACACA GCCGGCCCCT 
AATGCCAGCC CCATGAAGCG CTCCATCTCC ACACTGGCTC CACGCCCACA TGGGACTCAG 
CTTTGCAGCA CAGTTCTGGA CCGGCCTCCT CCTAGCCAGG CATCACATCA CCACCACCAC 
CGCTGCCACC GGCGCAGAGA CAAGAAGCAA AGGTCCCTGG AAAAGGGGCC CAGCCTGTCT 
GTTGACCCAG AAGGTGCACC AAGCACTGCT GCAGGACCTG GTCTGCCCCA TGGAGAAGGA 
TCCACCGCCT GCCGGCGGGA CCGTAAACAG GAGCGAGGCC GGTCCCAGGA GCGGAGGCAG 
CCCTCATCTT CCTCTTCAGA GAAGCAGCGC TTCTATTCCT GTGACCGCTT TGGGAGCCGG 
GAGCCCCCGC AACTGATGCC CTCACTCAGT AGCCACCCCA CATCGCCAAC AGCGGCGCTA 
GAGCCAGCAC CCCACCCACA GGGCAGTGGT TCCGTTAATG GGAGCCCCTT GATGTCAACA 
TCCGGTGCTA GCACCCCGGG CCGAGGTGGG CGGAGGCAGC TCCCCCAGAC TCCTCTGACC 
CCACGCCCCA GCATCACCTA CAAGACCGCC AATTCCTCGC CTGTCCACTT TGCTGAGGGT 
CAGAGCGGCC TCCCAGCCTT CTCCCCTGGC CGTCTCAGCC GCGGCCTTTC TGAACACAAT 
GCCCTGCTCC AGAAAGAGCC CCTGAGCCAG CCTCTAGCTC CTGGCTCCCG AATTGGCTCT 
GACCCTTACC TAGGGCAGCG TCTGGACAGT GAGGCCTCCG CCCACACCCT GCCTGAGGAT 
ACACTCACCT TTGAAGAGGC AGTGGCCACC AACTCTGGCC GCTCCTCCAG GACTTCCTAT 
GTGTCCTCCC TCACTTCCCA ATCCCACCCT CTCCGCCGTG TACCCAATGG CTATCACTGC 
ACTTTGGGAC TCAGCACTGG CGTCCGGGCG CGGCACAGCT ACCACCACCC CGATCAGGAC 
CACTGGTGCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006497699.1
CDS	337..7008
Translation	MQDDTEPYFIGIFCFEAGIKIIALGFVFHKGSYLRNGWNVMDFVVVLTGILATAGTDFDLRTLRAVRVLRPLKLVSGIPSLQVVLKSIMKAMVPLLQIGLLLFFAILMFAIIGLEFYMGKFHKACFPNSTDTEPVGDFPCGKDPPARQCDGDTECREYWPGPNFGITNFDNILFAILTVFQCITMEGWTDILYNTNDAAGNTWNWLYFIPLIIIGSFFMLNLVLGVLSGEFAKERERVENRRAFLKLRRQQQIERELNGYLEWIFKAEEVMLAEEDKNAEEKSPLDAVLKRAATKKSRNDLIHAEEGEDRFVDLCAVGSPFARASLKSGKTESSSYFRRKEKMFRFFIRRMVKAQSFYWVVLCVVALNTLCVAMVHYNQPQRLTTALYFAEFVFLGLFLTEMSLKMYGLGPRSYFRSSFNCFDFGVIVGSIFEVVWAAIKPGTSFGISVLRALRLLRIFKVTKYWNSLRNLVVSLLNSMKSIISLLFLLFLFIVVFALLGMQLFGGQFNFQDETPTTNFDTFPAAILTVFQILTGEDWNAVMYHGIESQGGVSKGMFSSFYFIVLTLFGNYTLLNVFLAIAVDNLANAQELTKDEEEMEEAANQKLALQKAKEVAEVSPMSAANISIAAFVKQTRGTVSRSSSVSSVNSPRQQNSAKARSVWEQRASQLRLQNLRASCEALYSEMDPEERLRYASTRHVRPDMKTHMDRPLVVEPGRDGLRGPVGSKSKPEGTEATESADLPRRHHRHRDRDKTSATAPAGGEQDRTESTETGAREERARPRRSHSKETPGADTQVRCERSRRHHRRGSPEEATEREPRRHRAHRHAQDSSKEGTAPVLVPKGERRARHRGPRTGPREAENNEEPTRRHRARHKVPPTLQPPEREAAEKESNAVEGDKETRNHQPKEPHCDLEAIAVTGVGPLHMLPSTCLQKVDEQPEDADNQRNVTRMGSQPSDPSTTVHVPVTLTGPPGETPVVPSGNMNLEGQAEGKKEAEADDVLRRGPRPIVPYSSMFCLSPTNLLRRFCHYIVTMRYFEMVILVVIALSSIALAAEDPVRTDSFRNNALKYMDYIFTGVFTFEMVIKMIDLGLLLHPGAYFRDLWNILDFIVVSGALVAFAFSSFMGGSKGKDINTIKSLRVLRVLRPLKTIKRLPKLKAVFDCVVNSLKNVLNILIVYMLFMFIFAVIAVQLFKGKFFYCTDESKELERDCRGQYLDYEKEEVEAQPRQWKKYDFHYDNVLWALLTLFTVSTGEGWPMVLKHSVDATYEEQGPSPGFRMELSIFYVVYFVVFPFFFVNIFVALIIITFQEQGDKVMSECSLEKNERACIDFAISAKPLTRYMPQNKQSFQYKTWTFVVSPPFEYFIMAMIALNTVVLMMKFYDAPYEYELMLKCLNIVFTSMFSMECILKIIAFGVLNYFRDAWNVFDFVTVLGSITDILVTEIAETNNFINLSFLRLFRAARLIKLLRQGYTIRILLWTFVQSFKALPYVCLLIAMLFFIYAIIGMQVFGNIALDDDTSINRHNNFRTFLQALMLLFRSATGEAWHEIMLSCLGNRACDPHANASECGSDFAYFYFVSFIFLCSFLMLNLFVAVIMDNFEYLTRDSSILGPHHLDEFIRVWAEYDPAACGRISYNDMFEMLKHMSPPLGLGKKCPARVAYKRLVRMNMPISNEDMTVHFTSTLMALIRTALEIKLAPAGTKQHQCDAELRKEISSVWANLPQKTLDLLVPPHKPDEMTVGKVYAALMIFDFYKQNKTTRDQTHQAPGGLSQMGPVSLFHPLKATLEQTQPAVLRGARVFLRQKSATSLSNGGAIQTQESGIKESLSWGTQRTQDALYEARAPLERGHSAEIPVGQSGTLAVDVQMQNMTLRGPDGEPQPGLESQGRAASMPRLAAETQPAPNASPMKRSISTLAPRPHGTQLCSTVLDRPPPSQASHHHHHRCHRRRDKKQRSLEKGPSLSVDPEGAPSTAAGPGLPHGEGSTACRRDRKQERGRSQERRQPSSSSSEKQRFYSCDRFGSREPPQLMPSLSSHPTSPTAALEPAPHPQGSGSVNGSPLMSTSGASTPGRGGRRQLPQTPLTPRPSITYKTANSSPVHFAEGQSGLPAFSPGRLSRGLSEHNALLQKEPLSQPLAPGSRIGSDPYLGQRLDSEASAHTLPEDTLTFEEAVATNSGRSSRTSYVSSLTSQSHPLRRVPNGYHCTLGLSTGVRARHSYHHPDQDHWC

Target ORF information:

RefSeq Version	XM_006497636.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus calcium channel, voltage-dependent, N type, alpha 1B subunit (Cacna1b), transcript variant X14, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006497636.3

ORF Insert Sequence:

ATGCAGGATG ACACGGAGCC TTACTTCATC GGGATCTTTT GCTTCGAGGC GGGCATCAAG 
ATCATAGCCC TGGGCTTTGT TTTCCACAAG GGCTCCTACC TTCGGAACGG CTGGAATGTC 
ATGGACTTCG TGGTGGTCCT CACGGGGATT CTCGCCACAG CTGGAACTGA CTTTGACCTG 
CGCACACTGA GGGCTGTGCG TGTGCTTAGG CCCCTGAAGC TGGTGTCTGG AATTCCAAGC 
TTGCAGGTGG TGCTTAAGTC CATCATGAAG GCCATGGTCC CGCTGCTGCA GATTGGGCTG 
CTGCTCTTCT TTGCCATCCT CATGTTTGCC ATCATCGGCC TCGAATTCTA TATGGGCAAA 
TTCCATAAGG CCTGTTTCCC CAACAGCACA GATACAGAGC CTGTGGGTGA CTTTCCCTGT 
GGCAAAGATC CCCCTGCTCG TCAGTGTGAT GGTGACACCG AATGCCGGGA GTACTGGCCA 
GGACCCAACT TTGGCATCAC CAATTTTGAC AACATCCTGT TTGCCATCTT GACAGTGTTC 
CAGTGTATCA CCATGGAGGG CTGGACTGAC ATCCTCTACA ATACAAATGA TGCGGCTGGC 
AACACATGGA ACTGGTTGTA CTTCATCCCC CTCATCATCA TTGGCTCCTT CTTCATGCTC 
AACCTGGTGC TGGGTGTGCT TTCCGGAGAG TTTGCCAAGG AGCGGGAGCG AGTCGAGAAC 
CGCCGCGCCT TCCTGAAGCT CCGCAGGCAG CAGCAGATTG AGCGAGAGCT GAATGGGTAC 
TTGGAGTGGA TCTTCAAGGC AGAGGAAGTC ATGTTGGCAG AGGAGGACAA GAATGCAGAA 
GAGAAATCCC CTTTGGATGC AGTGTTGAAG AGAGCTGCCA CCAAGAAGAG CCGAAATGAC 
CTCATCCATG CAGAAGAGGG GGAGGACCGG TTTGTAGACC TCTGTGCAGT TGGGTCTCCA 
TTTGCTCGTG CCAGCCTCAA GAGTGGGAAG ACGGAGAGCT CATCGTACTT CCGGAGAAAG 
GAGAAGATGT TCCGGTTCTT TATCCGGCGT ATGGTGAAAG CACAGAGCTT CTACTGGGTG 
GTACTGTGTG TGGTGGCCCT GAACACACTG TGTGTGGCCA TGGTGCACTA TAATCAGCCT 
CAGCGGCTTA CCACTGCACT GTACTTTGCA GAGTTTGTTT TCCTGGGTCT CTTCCTCACA 
GAGATGTCCC TGAAGATGTA TGGCCTAGGG CCCAGAAGTT ACTTCAGGTC TTCCTTCAAC 
TGCTTTGACT TTGGGGTGAT TGTGGGGAGT ATCTTTGAAG TAGTCTGGGC TGCCATCAAG 
CCAGGAACCT CCTTTGGAAT CAGTGTGCTG CGGGCTCTGC GACTGCTGAG GATATTCAAA 
GTTACCAAGT ATTGGAACTC TCTGAGGAAC CTGGTGGTTT CCCTCCTCAA TTCCATGAAG 
TCCATCATCA GCCTTCTCTT CCTGCTTTTC CTCTTCATCG TGGTCTTCGC TCTGTTGGGG 
ATGCAGCTGT TTGGGGGACA GTTCAACTTT CAAGATGAGA CTCCAACCAC CAATTTTGAT 
ACCTTCCCAG CTGCCATCCT CACTGTCTTT CAGATCCTGA CAGGAGAGGA TTGGAATGCC 
GTAATGTATC ATGGGATTGA GTCGCAAGGT GGAGTCAGCA AAGGCATGTT TTCTTCCTTT 
TACTTCATCG TCCTGACACT GTTTGGAAAC TACACCCTGC TGAATGTTTT TCTGGCCATT 
GCTGTGGACA ACCTTGCCAA TGCCCAGGAG TTGACCAAGG ATGAAGAGGA GATGGAAGAA 
GCAGCCAATC AGAAACTTGC TCTTCAGAAG GCCAAAGAAG TAGCTGAAGT CAGCCCCATG 
TCTGCTGCCA ATATCTCCAT CGCTGCTTTT GTAAAGCAAA CTCGAGGTAC TGTATCTCGC 
AGCTCATCTG TCTCCAGCGT AAACTCACCC AGGCAGCAGA ACTCGGCCAA GGCGCGCTCA 
GTATGGGAGC AGCGGGCCAG TCAGCTAAGG CTCCAGAATC TGCGTGCCAG CTGTGAGGCA 
TTGTACAGTG AGATGGACCC TGAGGAGCGC CTGCGTTATG CCAGCACGCG CCATGTGAGG 
CCAGACATGA AGACACACAT GGACCGACCC CTAGTGGTGG AGCCTGGTCG AGATGGCTTG 
CGGGGACCCG TTGGGAGCAA GTCAAAGCCT GAAGGCACGG AGGCCACAGA AAGCGCGGAC 
CTACCTCGCA GGCACCACCG GCACCGTGAT AGGGACAAGA CCTCAGCCAC AGCACCTGCT 
GGAGGCGAAC AGGACAGGAC AGAAAGCACC GAGACCGGGG CCCGGGAGGA ACGTGCGCGC 
CCTCGTCGAA GTCACAGCAA GGAGACTCCA GGGGCTGACA CGCAAGTGCG CTGTGAGCGC 
AGTAGACGTC ACCACCGGCG CGGCTCCCCG GAGGAGGCCA CTGAACGGGA GCCTCGGCGC 
CACCGTGCCC ACCGGCATGC ACAGGACTCA AGCAAGGAGG GCACGGCGCC GGTGCTTGTA 
CCCAAGGGTG AGCGACGAGC AAGACACCGA GGCCCACGCA CGGGTCCACG TGAGGCAGAG 
AACAACGAGG AGCCCACACG CAGGCACCGT GCAAGGCATA AGGTGCCACC CACACTGCAG 
CCCCCAGAGA GGGAGGCTGC AGAGAAGGAG AGCAACGCGG TGGAAGGGGA TAAGGAAACC 
CGAAATCACC AGCCCAAGGA ACCTCACTGT GACCTGGAGG CCATTGCAGT TACAGGTGTG 
GGCCCTCTGC ACATGCTGCC CAGCACCTGT CTCCAGAAAG TGGACGAGCA ACCAGAGGAT 
GCAGACAACC AGCGTAATGT CACCCGGATG GGCAGTCAGC CCTCAGATCC CAGCACCACT 
GTGCATGTCC CAGTGACACT GACAGGCCCT CCTGGGGAGA CCCCTGTAGT TCCCAGTGGT 
AACATGAACC TGGAAGGCCA AGCAGAGGGC AAGAAGGAGG CAGAGGCGGA TGATGTGCTG 
AGAAGAGGCC CCAGGCCCAT CGTTCCCTAC AGCTCCATGT TTTGTCTCAG CCCCACCAAC 
CTGCTTCGTC GCTTCTGCCA TTACATTGTG ACCATGCGGT ACTTTGAGAT GGTAATTCTT 
GTGGTCATTG CCTTGAGCAG CATTGCCCTG GCTGCAGAGG ATCCTGTGCG GACAGATTCA 
TTCAGGAACA ACGCTTTAAA GTACATGGAT TACATCTTTA CAGGAGTCTT CACCTTTGAA 
ATGGTCATAA AGATGATAGA CTTGGGCTTG CTGCTGCACC CTGGTGCCTA CTTCCGGGAC 
CTGTGGAACA TTCTGGACTT CATTGTTGTC AGTGGAGCCC TGGTGGCATT TGCGTTCTCG 
AGCTTCATGG GAGGATCCAA AGGGAAAGAC ATCAATACCA TCAAGTCTCT GAGAGTCCTG 
CGTGTCCTGA GGCCCCTCAA GACCATCAAG CGGCTGCCTA AGCTCAAGGC TGTCTTTGAC 
TGTGTGGTGA ACTCCCTGAA GAACGTCTTG AACATCCTGA TTGTCTACAT GCTCTTCATG 
TTCATATTTG CCGTCATTGC CGTCCAGCTC TTCAAAGGGA AGTTCTTTTA CTGTACTGAT 
GAATCCAAGG AGCTGGAGAG GGACTGCCGG GGTCAGTATT TGGATTATGA GAAGGAAGAA 
GTAGAAGCCC AGCCAAGGCA GTGGAAGAAA TATGACTTCC ACTATGACAA TGTTCTCTGG 
GCCTTGTTGA CGCTGTTCAC AGTGTCCACG GGAGAGGGGT GGCCCATGGT GCTGAAACAC 
TCTGTGGATG CCACCTATGA GGAACAGGGG CCCAGTCCCG GGTTCCGGAT GGAGCTCTCC 
ATCTTCTACG TGGTCTACTT TGTGGTCTTC CCTTTTTTCT TTGTCAACAT CTTTGTGGCC 
TTGATCATTA TCACCTTCCA GGAACAGGGA GATAAGGTGA TGTCTGAATG CAGCTTAGAA 
AAGAATGAGA GGGCTTGCAT TGATTTTGCC ATCAGTGCCA AGCCCCTGAC ACGGTACATG 
CCTCAAAACA AACAGTCGTT CCAGTATAAG ACATGGACAT TCGTGGTCTC TCCACCCTTT 
GAGTACTTCA TCATGGCTAT GATAGCCCTC AACACAGTGG TGCTGATGAT GAAGTTCTAT 
GATGCACCTT ATGAGTACGA GCTGATGCTG AAATGCCTGA ACATTGTCTT CACATCCATG 
TTCTCGATGG AGTGCATACT GAAGATCATC GCCTTTGGGG TATTGAACTA CTTCAGAGAT 
GCCTGGAACG TCTTTGACTT TGTCACGGTT TTGGGAAGTA TTACTGATAT TTTAGTAACA 
GAGATAGCGG AAACGAACAA CTTCATCAAC CTAAGCTTCC TTCGCCTCTT CCGGGCGGCA 
CGGCTGATCA AGCTGCTTCG CCAGGGCTAC ACCATCCGCA TCCTATTGTG GACCTTCGTC 
CAGTCCTTTA AGGCGCTGCC CTACGTGTGC CTCCTCATTG CCATGCTGTT CTTCATCTAC 
GCCATCATCG GCATGCAGGT TTTTGGAAAC ATTGCCCTTG ATGATGACAC CAGTATCAAC 
CGACACAACA ACTTCCGGAC ATTTCTGCAA GCCTTAATGC TATTGTTCAG GAGTGCCACT 
GGGGAGGCCT GGCATGAGAT CATGCTGTCT TGTCTGGGCA ACCGGGCCTG TGACCCACAT 
GCCAACGCCA GTGAGTGCGG GAGCGACTTT GCCTATTTTT ATTTTGTCTC CTTCATCTTC 
CTCTGTTCCT TTCTGATGTT GAACCTCTTT GTTGCTGTAA TCATGGACAA TTTTGAGTAC 
CTCACTCGGG ACTCTTCCAT CCTAGGGCCT CACCACTTAG ACGAATTCAT TCGAGTCTGG 
GCTGAATACG ACCCAGCTGC GTGTGGGCGC ATCAGTTACA ATGACATGTT TGAGATGCTG 
AAACACATGT CCCCACCTCT GGGGTTGGGG AAGAAATGCC CGGCTCGAGT TGCATACAAG 
CGCCTGGTTC GCATGAACAT GCCCATATCC AATGAGGACA TGACGGTGCA CTTTACGTCC 
ACACTGATGG CCCTCATCCG GACAGCACTG GAGATCAAGC TTGCCCCAGC GGGGACGAAG 
CAGCACCAGT GTGATGCTGA GCTGAGAAAA GAGATCTCTT CTGTGTGGGC TAATCTGCCC 
CAGAAGACTC TGGACTTACT GGTACCACCC CACAAACCTG ACGAGATGAC AGTGGGGAAG 
GTCTATGCTG CTCTCATGAT ATTTGACTTC TACAAACAGA ACAAAACCAC CAGAGATCAG 
ACTCACCAAG CTCCCGGAGG CCTGTCCCAG ATGGGTCCCG TTTCCCTGTT CCACCCTCTG 
AAGGCCACCC TGGAACAGAC ACAGCCCGCT GTGCTTCGAG GAGCTCGGGT TTTCCTTCGG 
CAAAAGAGTG CAACTTCCCT CAGCAATGGG GGTGCCATAC AAACCCAGGA AAGTGGCATC 
AAGGAGTCGC TGTCCTGGGG CACGCAGAGG ACCCAAGATG CACTTTATGA GGCCAGAGCA 
CCTCTAGAAC GTGGCCATTC TGCAGAGATC CCTGTGGGGC AGTCAGGAAC ACTGGCTGTG 
GATGTCCAGA TGCAGAACAT GACACTGAGA GGACCAGATG GGGAGCCCCA GCCTGGGCTG 
GAAAGCCAAG GCAGAGCTGC CTCTATGCCA CGCCTAGCGG CAGAAACACA GCCGGCCCCT 
AATGCCAGCC CCATGAAGCG CTCCATCTCC ACACTGGCTC CACGCCCACA TGGGACTCAG 
CTTTGCAGCA CAGTTCTGGA CCGGCCTCCT CCTAGCCAGG CATCACATCA CCACCACCAC 
CGCTGCCACC GGCGCAGAGA CAAGAAGCAA AGGTCCCTGG AAAAGGGGCC CAGCCTGTCT 
GTTGACCCAG AAGGTGCACC AAGCACTGCT GCAGGACCTG GTCTGCCCCA TGGAGAAGGA 
TCCACCGCCT GCCGGCGGGA CCGTAAACAG GAGCGAGGCC GGTCCCAGGA GCGGAGGCAG 
CCCTCATCTT CCTCTTCAGA GAAGCAGCGC TTCTATTCCT GTGACCGCTT TGGGAGCCGG 
GAGCCCCCGC AACTGATGCC CTCACTCAGT AGCCACCCCA CATCGCCAAC AGCGGCGCTA 
GAGCCAGCAC CCCACCCACA GGGCAGTGGT TCCGTTAATG GGAGCCCCTT GATGTCAACA 
TCCGGTGCTA GCACCCCGGG CCGAGGTGGG CGGAGGCAGC TCCCCCAGAC TCCTCTGACC 
CCACGCCCCA GCATCACCTA CAAGACCGCC AATTCCTCGC CTGTCCACTT TGCTGAGGGT 
CAGAGCGGCC TCCCAGCCTT CTCCCCTGGC CGTCTCAGCC GCGGCCTTTC TGAACACAAT 
GCCCTGCTCC AGAAAGAGCC CCTGAGCCAG CCTCTAGCTC CTGGCTCCCG AATTGGCTCT 
GACCCTTACC TAGGGCAGCG TCTGGACAGT GAGGCCTCCG CCCACACCCT GCCTGAGGAT 
ACACTCACCT TTGAAGAGGC AGTGGCCACC AACTCTGGCC GCTCCTCCAG GACTTCCTAT 
GTGTCCTCCC TCACTTCCCA ATCCCACCCT CTCCGCCGTG TACCCAATGG CTATCACTGC 
ACTTTGGGAC TCAGCACTGG CGTCCGGGCG CGGCACAGCT ACCACCACCC CGATCAGGAC 
CACTGGTGCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cacna1b ( NM_007579.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_031605.2 Mus musculus calcium channel, voltage-dependent, N type, alpha 1B subunit (Cacna1b), transcript variant 2, mRNA.

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CloneID	OMu19787
Clone ID Related Accession (Same CDS sequence)	NM_007579.3
Accession Version	NM_007579.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6867bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-27
Organism	Mus musculus(house mouse)
Product	voltage-dependent N-type calcium channel subunit alpha-1B isoform 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AL732525.13, BX294112.6 and AL732546.13. On Oct 18, 2013 this sequence version replaced NM_007579.2. Transcript Variant: This variant (2) lacks an alternate in-frame exon compared to variant 1. The resulting protein (isoform 2) is shorter compared to isoform 1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END##

ATGGTCCGCT TCGGGGACGA GCTAGGCGGC CGCTATGGGG GCACCGGCGG CGGGGAGCGG 
GCTCGGGGTG GCGGGGCCGG CGGGGCGGGT GGCCCGGGCC AGGGGGGTCT GCCGCCGGGC 
CAGCGGGTCC TGTACAAGCA GTCCATTGCG CAGCGCGCAC GGACTATGGC CCTGTACAAC 
CCCATCCCAG TCAAGCAGAA CTGCTTCACC GTCAACCGCT CGCTCTTCGT CTTCAGCGAG 
GACAACGTCG TCCGCAAATA CGCTAAGCGC ATCACCGAAT GGCCGCCCTT CGAATACATG 
ATCCTGGCCA CCATCATCGC CAACTGCATT GTTCTGGCCC TGGAGCAGCA CCTCCCTGAT 
GGGGACAAGA CTCCCATGTC TGAGCGACTA GATGACACGG AGCCTTACTT CATCGGGATC 
TTTTGCTTCG AGGCGGGCAT CAAGATCATA GCCCTGGGCT TTGTTTTCCA CAAGGGCTCC 
TACCTTCGGA ACGGCTGGAA TGTCATGGAC TTCGTGGTGG TCCTCACGGG GATTCTCGCC 
ACAGCTGGAA CTGACTTTGA CCTGCGCACA CTGAGGGCTG TGCGTGTGCT TAGGCCCCTG 
AAGCTGGTGT CTGGAATTCC AAGCTTGCAG GTGGTGCTTA AGTCCATCAT GAAGGCCATG 
GTCCCGCTGC TGCAGATTGG GCTGCTGCTC TTCTTTGCCA TCCTCATGTT TGCCATCATC 
GGCCTCGAAT TCTATATGGG CAAATTCCAT AAGGCCTGTT TCCCCAACAG CACAGATACA 
GAGCCTGTGG GTGACTTTCC CTGTGGCAAA GATCCCCCTG CTCGTCAGTG TGATGGTGAC 
ACCGAATGCC GGGAGTACTG GCCAGGACCC AACTTTGGCA TCACCAATTT TGACAACATC 
CTGTTTGCCA TCTTGACAGT GTTCCAGTGT ATCACCATGG AGGGCTGGAC TGACATCCTC 
TACAATACAA ATGATGCGGC TGGCAACACA TGGAACTGGT TGTACTTCAT CCCCCTCATC 
ATCATTGGCT CCTTCTTCAT GCTCAACCTG GTGCTGGGTG TGCTTTCCGG AGAGTTTGCC 
AAGGAGCGGG AGCGAGTCGA GAACCGCCGC GCCTTCCTGA AGCTCCGCAG GCAGCAGCAG 
ATTGAGCGAG AGCTGAATGG GTACTTGGAG TGGATCTTCA AGGCAGAGGA AGTCATGTTG 
GCAGAGGAGG ACAAGAATGC AGAAGAGAAA TCCCCTTTGG ATGTGTTGAA GAGAGCTGCC 
ACCAAGAAGA GCCGAAATGA CCTCATCCAT GCAGAAGAGG GGGAGGACCG GTTTGTAGAC 
CTCTGTGCAG TTGGGTCTCC ATTTGCTCGT GCCAGCCTCA AGAGTGGGAA GACGGAGAGC 
TCATCGTACT TCCGGAGAAA GGAGAAGATG TTCCGGTTCT TTATCCGGCG TATGGTGAAA 
GCACAGAGCT TCTACTGGGT GGTACTGTGT GTGGTGGCCC TGAACACACT GTGTGTGGCC 
ATGGTGCACT ATAATCAGCC TCAGCGGCTT ACCACTGCAC TGTACTTTGC AGAGTTTGTT 
TTCCTGGGTC TCTTCCTCAC AGAGATGTCC CTGAAGATGT ATGGCCTAGG GCCCAGAAGT 
TACTTCAGGT CTTCCTTCAA CTGCTTTGAC TTTGGGGTGA TTGTGGGGAG TATCTTTGAA 
GTAGTCTGGG CTGCCATCAA GCCAGGAACC TCCTTTGGAA TCAGTGTGCT GCGGGCTCTG 
CGACTGCTGA GGATATTCAA AGTTACCAAG TATTGGAACT CTCTGAGGAA CCTGGTGGTT 
TCCCTCCTCA ATTCCATGAA GTCCATCATC AGCCTTCTCT TCCTGCTTTT CCTCTTCATC 
GTGGTCTTCG CTCTGTTGGG GATGCAGCTG TTTGGGGGAC AGTTCAACTT TCAAGATGAG 
ACTCCAACCA CCAATTTTGA TACCTTCCCA GCTGCCATCC TCACTGTCTT TCAGATCCTG 
ACAGGAGAGG ATTGGAATGC CGTAATGTAT CATGGGATTG AGTCGCAAGG TGGAGTCAGC 
AAAGGCATGT TTTCTTCCTT TTACTTCATC GTCCTGACAC TGTTTGGAAA CTACACCCTG 
CTGAATGTTT TTCTGGCCAT TGCTGTGGAC AACCTTGCCA ATGCCCAGGA GTTGACCAAG 
GATGAAGAGG AGATGGAAGA AGCAGCCAAT CAGAAACTTG CTCTTCAGAA GGCCAAAGAA 
GTAGCTGAAG TCAGCCCCAT GTCTGCTGCC AATATCTCCA TCGCTGCGCA GCAGAACTCG 
GCCAAGGCGC GCTCAGTATG GGAGCAGCGG GCCAGTCAGC TAAGGCTCCA GAATCTGCGT 
GCCAGCTGTG AGGCATTGTA CAGTGAGATG GACCCTGAGG AGCGCCTGCG TTATGCCAGC 
ACGCGCCATG TGAGGCCAGA CATGAAGACA CACATGGACC GACCCCTAGT GGTGGAGCCT 
GGTCGAGATG GCTTGCGGGG ACCCGTTGGG AGCAAGTCAA AGCCTGAAGG CACGGAGGCC 
ACAGAAAGCG CGGACCTACC TCGCAGGCAC CACCGGCACC GTGATAGGGA CAAGACCTCA 
GCCACAGCAC CTGCTGGAGG CGAACAGGAC AGGACAGAAA GCACCGAGAC CGGGGCCCGG 
GAGGAACGTG CGCGCCCTCG TCGAAGTCAC AGCAAGGAGA CTCCAGGGGC TGACACGCAA 
GTGCGCTGTG AGCGCAGTAG ACGTCACCAC CGGCGCGGCT CCCCGGAGGA GGCCACTGAA 
CGGGAGCCTC GGCGCCACCG TGCCCACCGG CATGCACAGG ACTCAAGCAA GGAGGGCACG 
GCGCCGGTGC TTGTACCCAA GGGTGAGCGA CGAGCAAGAC ACCGAGGCCC ACGCACGGGT 
CCACGTGAGG CAGAGAACAA CGAGGAGCCC ACACGCAGGC ACCGTGCAAG GCATAAGGTG 
CCACCCACAC TGCAGCCCCC AGAGAGGGAG GCTGCAGAGA AGGAGAGCAA CGCGGTGGAA 
GGGGATAAGG AAACCCGAAA TCACCAGCCC AAGGAACCTC ACTGTGACCT GGAGGCCATT 
GCAGTTACAG GTGTGGGCCC TCTGCACATG CTGCCCAGCA CCTGTCTCCA GAAAGTGGAC 
GAGCAACCAG AGGATGCAGA CAACCAGCGT AATGTCACCC GGATGGGCAG TCAGCCCTCA 
GATCCCAGCA CCACTGTGCA TGTCCCAGTG ACACTGACAG GCCCTCCTGG GGAGACCCCT 
GTAGTTCCCA GTGGTAACAT GAACCTGGAA GGCCAAGCAG AGGGCAAGAA GGAGGCAGAG 
GCGGATGATG TGCTGAGAAG AGGCCCCAGG CCCATCGTTC CCTACAGCTC CATGTTTTGT 
CTCAGCCCCA CCAACCTGCT TCGTCGCTTC TGCCATTACA TTGTGACCAT GCGGTACTTT 
GAGATGGTAA TTCTTGTGGT CATTGCCTTG AGCAGCATTG CCCTGGCTGC AGAGGATCCT 
GTGCGGACAG ATTCATTCAG GAACAACGCT TTAAAGTACA TGGATTACAT CTTTACAGGA 
GTCTTCACCT TTGAAATGGT CATAAAGATG ATAGACTTGG GCTTGCTGCT GCACCCTGGT 
GCCTACTTCC GGGACCTGTG GAACATTCTG GACTTCATTG TTGTCAGTGG AGCCCTGGTG 
GCATTTGCGT TCTCAGGATC CAAAGGGAAA GACATCAATA CCATCAAGTC TCTGAGAGTC 
CTGCGTGTCC TGAGGCCCCT CAAGACCATC AAGCGGCTGC CTAAGCTCAA GGCTGTCTTT 
GACTGTGTGG TGAACTCCCT GAAGAACGTC TTGAACATCC TGATTGTCTA CATGCTCTTC 
ATGTTCATAT TTGCCGTCAT TGCCGTCCAG CTCTTCAAAG GGAAGTTCTT TTACTGTACT 
GATGAATCCA AGGAGCTGGA GAGGGACTGC CGGGGTCAGT ATTTGGATTA TGAGAAGGAA 
GAAGTAGAAG CCCAGCCAAG GCAGTGGAAG AAATATGACT TCCACTATGA CAATGTTCTC 
TGGGCCTTGT TGACGCTGTT CACAGTGTCC ACGGGAGAGG GGTGGCCCAT GGTGCTGAAA 
CACTCTGTGG ATGCCACCTA TGAGGAACAG GGGCCCAGTC CCGGGTTCCG GATGGAGCTC 
TCCATCTTCT ACGTGGTCTA CTTTGTGGTC TTCCCTTTTT TCTTTGTCAA CATCTTTGTG 
GCCTTGATCA TTATCACCTT CCAGGAACAG GGAGATAAGG TGATGTCTGA ATGCAGCTTA 
GAAAAGAATG AGAGGGCTTG CATTGATTTT GCCATCAGTG CCAAGCCCCT GACACGGTAC 
ATGCCTCAAA ACAAACAGTC GTTCCAGTAT AAGACATGGA CATTCGTGGT CTCTCCACCC 
TTTGAGTACT TCATCATGGC TATGATAGCC CTCAACACAG TGGTGCTGAT GATGAAGTTC 
TATGATGCAC CTTATGAGTA CGAGCTGATG CTGAAATGCC TGAACATTGT CTTCACATCC 
ATGTTCTCGA TGGAGTGCAT ACTGAAGATC ATCGCCTTTG GGGTATTGAA CTACTTCAGA 
GATGCCTGGA ACGTCTTTGA CTTTGTCACG GTTTTGGGAA GTATTACTGA TATTTTAGTA 
ACAGAGATAG CGGAAACGAA CAACTTCATC AACCTAAGCT TCCTTCGCCT CTTCCGGGCG 
GCACGGCTGA TCAAGCTGCT TCGCCAGGGC TACACCATCC GCATCCTATT GTGGACCTTC 
GTCCAGTCCT TTAAGGCGCT GCCCTACGTG TGCCTCCTCA TTGCCATGCT GTTCTTCATC 
TACGCCATCA TCGGCATGCA GGTTTTTGGA AACATTGCCC TTGATGATGA CACCAGTATC 
AACCGACACA ACAACTTCCG GACATTTCTG CAAGCCTTAA TGCTATTGTT CAGGAGTGCC 
ACTGGGGAGG CCTGGCATGA GATCATGCTG TCTTGTCTGG GCAACCGGGC CTGTGACCCA 
CATGCCAACG CCAGTGAGTG CGGGAGCGAC TTTGCCTATT TTTATTTTGT CTCCTTCATC 
TTCCTCTGTT CCTTTCTGAT GTTGAACCTC TTTGTTGCTG TAATCATGGA CAATTTTGAG 
TACCTCACTC GGGACTCTTC CATCCTAGGG CCTCACCACT TAGACGAATT CATTCGAGTC 
TGGGCTGAAT ACGACCCAGC TGCGTGTGGG CGCATCAGTT ACAATGACAT GTTTGAGATG 
CTGAAACACA TGTCCCCACC TCTGGGGTTG GGGAAGAAAT GCCCGGCTCG AGTTGCATAC 
AAGCGCCTGG TTCGCATGAA CATGCCCATA TCCAATGAGG ACATGACGGT GCACTTTACG 
TCCACACTGA TGGCCCTCAT CCGGACAGCA CTGGAGATCA AGCTTGCCCC AGCTGACGAG 
ATGACAGTGG GGAAGGTCTA TGCTGCTCTC ATGATATTTG ACTTCTACAA ACAGAACAAA 
ACCACCAGAG ATCAGACTCA CCAAGCTCCC GGAGGCCTGT CCCAGATGGG TCCCGTTTCC 
CTGTTCCACC CTCTGAAGGC CACCCTGGAA CAGACACAGC CCGCTGTGCT TCGAGGAGCT 
CGGGTTTTCC TTCGGCAAAA GAGTGCAACT TCCCTCAGCA ATGGGGGTGC CATACAAACC 
CAGGAAAGTG GCATCAAGGA GTCGCTGTCC TGGGGCACGC AGAGGACCCA AGATGCACTT 
TATGAGGCCA GAGCACCTCT AGAACGTGGC CATTCTGCAG AGATCCCTGT GGGGCAGTCA 
GGAACACTGG CTGTGGATGT CCAGATGCAG AACATGACAC TGAGAGGACC AGATGGGGAG 
CCCCAGCCTG GGCTGGAAAG CCAAGGCAGA GCTGCCTCTA TGCCACGCCT AGCGGCAGAA 
ACACAGCCGG CCCCTAATGC CAGCCCCATG AAGCGCTCCA TCTCCACACT GGCTCCACGC 
CCACATGGGA CTCAGCTTTG CAGCACAGTT CTGGACCGGC CTCCTCCTAG CCAGGCATCA 
CATCACCACC ACCACCGCTG CCACCGGCGC AGAGACAAGA AGCAAAGGTC CCTGGAAAAG 
GGGCCCAGCC TGTCTGTTGA CCCAGAAGGT GCACCAAGCA CTGCTGCAGG ACCTGGTCTG 
CCCCATGGAG AAGGATCCAC CGCCTGCCGG CGGGACCGTA AACAGGAGCG AGGCCGGTCC 
CAGGAGCGGA GGCAGCCCTC ATCTTCCTCT TCAGAGAAGC AGCGCTTCTA TTCCTGTGAC 
CGCTTTGGGA GCCGGGAGCC CCCGCAACTG ATGCCCTCAC TCAGTAGCCA CCCCACATCG 
CCAACAGCGG CGCTAGAGCC AGCACCCCAC CCACAGGGCA GTGGTTCCGT TAATGGGAGC 
CCCTTGATGT CAACATCCGG TGCTAGCACC CCGGGCCGAG GTGGGCGGAG GCAGCTCCCC 
CAGACTCCTC TGACCCCACG CCCCAGCATC ACCTACAAGA CCGCCAATTC CTCGCCTGTC 
CACTTTGCTG AGGGTCAGAG CGGCCTCCCA GCCTTCTCCC CTGGCCGTCT CAGCCGCGGC 
CTTTCTGAAC ACAATGCCCT GCTCCAGAAA GAGCCCCTGA GCCAGCCTCT AGCTCCTGGC 
TCCCGAATTG GCTCTGACCC TTACCTAGGG CAGCGTCTGG ACAGTGAGGC CTCCGCCCAC 
ACCCTGCCTG AGGATACACT CACCTTTGAA GAGGCAGTGG CCACCAACTC TGGCCGCTCC 
TCCAGGACTT CCTATGTGTC CTCCCTCACT TCCCAATCCC ACCCTCTCCG CCGTGTACCC 
AATGGCTATC ACTGCACTTT GGGACTCAGC ACTGGCGTCC GGGCGCGGCA CAGCTACCAC 
CACCCCGATC AGGACCACTG GTGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_031605.2
CDS	106..6972
Translation	MVRFGDELGGRYGGTGGGERARGGGAGGAGGPGQGGLPPGQRVLYKQSIAQRARTMALYNPIPVKQNCFTVNRSLFVFSEDNVVRKYAKRITEWPPFEYMILATIIANCIVLALEQHLPDGDKTPMSERLDDTEPYFIGIFCFEAGIKIIALGFVFHKGSYLRNGWNVMDFVVVLTGILATAGTDFDLRTLRAVRVLRPLKLVSGIPSLQVVLKSIMKAMVPLLQIGLLLFFAILMFAIIGLEFYMGKFHKACFPNSTDTEPVGDFPCGKDPPARQCDGDTECREYWPGPNFGITNFDNILFAILTVFQCITMEGWTDILYNTNDAAGNTWNWLYFIPLIIIGSFFMLNLVLGVLSGEFAKERERVENRRAFLKLRRQQQIERELNGYLEWIFKAEEVMLAEEDKNAEEKSPLDVLKRAATKKSRNDLIHAEEGEDRFVDLCAVGSPFARASLKSGKTESSSYFRRKEKMFRFFIRRMVKAQSFYWVVLCVVALNTLCVAMVHYNQPQRLTTALYFAEFVFLGLFLTEMSLKMYGLGPRSYFRSSFNCFDFGVIVGSIFEVVWAAIKPGTSFGISVLRALRLLRIFKVTKYWNSLRNLVVSLLNSMKSIISLLFLLFLFIVVFALLGMQLFGGQFNFQDETPTTNFDTFPAAILTVFQILTGEDWNAVMYHGIESQGGVSKGMFSSFYFIVLTLFGNYTLLNVFLAIAVDNLANAQELTKDEEEMEEAANQKLALQKAKEVAEVSPMSAANISIAAQQNSAKARSVWEQRASQLRLQNLRASCEALYSEMDPEERLRYASTRHVRPDMKTHMDRPLVVEPGRDGLRGPVGSKSKPEGTEATESADLPRRHHRHRDRDKTSATAPAGGEQDRTESTETGAREERARPRRSHSKETPGADTQVRCERSRRHHRRGSPEEATEREPRRHRAHRHAQDSSKEGTAPVLVPKGERRARHRGPRTGPREAENNEEPTRRHRARHKVPPTLQPPEREAAEKESNAVEGDKETRNHQPKEPHCDLEAIAVTGVGPLHMLPSTCLQKVDEQPEDADNQRNVTRMGSQPSDPSTTVHVPVTLTGPPGETPVVPSGNMNLEGQAEGKKEAEADDVLRRGPRPIVPYSSMFCLSPTNLLRRFCHYIVTMRYFEMVILVVIALSSIALAAEDPVRTDSFRNNALKYMDYIFTGVFTFEMVIKMIDLGLLLHPGAYFRDLWNILDFIVVSGALVAFAFSGSKGKDINTIKSLRVLRVLRPLKTIKRLPKLKAVFDCVVNSLKNVLNILIVYMLFMFIFAVIAVQLFKGKFFYCTDESKELERDCRGQYLDYEKEEVEAQPRQWKKYDFHYDNVLWALLTLFTVSTGEGWPMVLKHSVDATYEEQGPSPGFRMELSIFYVVYFVVFPFFFVNIFVALIIITFQEQGDKVMSECSLEKNERACIDFAISAKPLTRYMPQNKQSFQYKTWTFVVSPPFEYFIMAMIALNTVVLMMKFYDAPYEYELMLKCLNIVFTSMFSMECILKIIAFGVLNYFRDAWNVFDFVTVLGSITDILVTEIAETNNFINLSFLRLFRAARLIKLLRQGYTIRILLWTFVQSFKALPYVCLLIAMLFFIYAIIGMQVFGNIALDDDTSINRHNNFRTFLQALMLLFRSATGEAWHEIMLSCLGNRACDPHANASECGSDFAYFYFVSFIFLCSFLMLNLFVAVIMDNFEYLTRDSSILGPHHLDEFIRVWAEYDPAACGRISYNDMFEMLKHMSPPLGLGKKCPARVAYKRLVRMNMPISNEDMTVHFTSTLMALIRTALEIKLAPADEMTVGKVYAALMIFDFYKQNKTTRDQTHQAPGGLSQMGPVSLFHPLKATLEQTQPAVLRGARVFLRQKSATSLSNGGAIQTQESGIKESLSWGTQRTQDALYEARAPLERGHSAEIPVGQSGTLAVDVQMQNMTLRGPDGEPQPGLESQGRAASMPRLAAETQPAPNASPMKRSISTLAPRPHGTQLCSTVLDRPPPSQASHHHHHRCHRRRDKKQRSLEKGPSLSVDPEGAPSTAAGPGLPHGEGSTACRRDRKQERGRSQERRQPSSSSSEKQRFYSCDRFGSREPPQLMPSLSSHPTSPTAALEPAPHPQGSGSVNGSPLMSTSGASTPGRGGRRQLPQTPLTPRPSITYKTANSSPVHFAEGQSGLPAFSPGRLSRGLSEHNALLQKEPLSQPLAPGSRIGSDPYLGQRLDSEASAHTLPEDTLTFEEAVATNSGRSSRTSYVSSLTSQSHPLRRVPNGYHCTLGLSTGVRARHSYHHPDQDHWC

Target ORF information:

RefSeq Version	NM_007579.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus calcium channel, voltage-dependent, N type, alpha 1B subunit (Cacna1b), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_007579.3

ORF Insert Sequence:

ATGGTCCGCT TCGGGGACGA GCTAGGCGGC CGCTATGGGG GCACCGGCGG CGGGGAGCGG 
GCTCGGGGTG GCGGGGCCGG CGGGGCGGGT GGCCCGGGCC AGGGGGGTCT GCCGCCGGGC 
CAGCGGGTCC TGTACAAGCA GTCCATTGCG CAGCGCGCAC GGACTATGGC CCTGTACAAC 
CCCATCCCAG TCAAGCAGAA CTGCTTCACC GTCAACCGCT CGCTCTTCGT CTTCAGCGAG 
GACAACGTCG TCCGCAAATA CGCTAAGCGC ATCACCGAAT GGCCGCCCTT CGAATACATG 
ATCCTGGCCA CCATCATCGC CAACTGCATT GTTCTGGCCC TGGAGCAGCA CCTCCCTGAT 
GGGGACAAGA CTCCCATGTC TGAGCGACTA GATGACACGG AGCCTTACTT CATCGGGATC 
TTTTGCTTCG AGGCGGGCAT CAAGATCATA GCCCTGGGCT TTGTTTTCCA CAAGGGCTCC 
TACCTTCGGA ACGGCTGGAA TGTCATGGAC TTCGTGGTGG TCCTCACGGG GATTCTCGCC 
ACAGCTGGAA CTGACTTTGA CCTGCGCACA CTGAGGGCTG TGCGTGTGCT TAGGCCCCTG 
AAGCTGGTGT CTGGAATTCC AAGCTTGCAG GTGGTGCTTA AGTCCATCAT GAAGGCCATG 
GTCCCGCTGC TGCAGATTGG GCTGCTGCTC TTCTTTGCCA TCCTCATGTT TGCCATCATC 
GGCCTCGAAT TCTATATGGG CAAATTCCAT AAGGCCTGTT TCCCCAACAG CACAGATACA 
GAGCCTGTGG GTGACTTTCC CTGTGGCAAA GATCCCCCTG CTCGTCAGTG TGATGGTGAC 
ACCGAATGCC GGGAGTACTG GCCAGGACCC AACTTTGGCA TCACCAATTT TGACAACATC 
CTGTTTGCCA TCTTGACAGT GTTCCAGTGT ATCACCATGG AGGGCTGGAC TGACATCCTC 
TACAATACAA ATGATGCGGC TGGCAACACA TGGAACTGGT TGTACTTCAT CCCCCTCATC 
ATCATTGGCT CCTTCTTCAT GCTCAACCTG GTGCTGGGTG TGCTTTCCGG AGAGTTTGCC 
AAGGAGCGGG AGCGAGTCGA GAACCGCCGC GCCTTCCTGA AGCTCCGCAG GCAGCAGCAG 
ATTGAGCGAG AGCTGAATGG GTACTTGGAG TGGATCTTCA AGGCAGAGGA AGTCATGTTG 
GCAGAGGAGG ACAAGAATGC AGAAGAGAAA TCCCCTTTGG ATGTGTTGAA GAGAGCTGCC 
ACCAAGAAGA GCCGAAATGA CCTCATCCAT GCAGAAGAGG GGGAGGACCG GTTTGTAGAC 
CTCTGTGCAG TTGGGTCTCC ATTTGCTCGT GCCAGCCTCA AGAGTGGGAA GACGGAGAGC 
TCATCGTACT TCCGGAGAAA GGAGAAGATG TTCCGGTTCT TTATCCGGCG TATGGTGAAA 
GCACAGAGCT TCTACTGGGT GGTACTGTGT GTGGTGGCCC TGAACACACT GTGTGTGGCC 
ATGGTGCACT ATAATCAGCC TCAGCGGCTT ACCACTGCAC TGTACTTTGC AGAGTTTGTT 
TTCCTGGGTC TCTTCCTCAC AGAGATGTCC CTGAAGATGT ATGGCCTAGG GCCCAGAAGT 
TACTTCAGGT CTTCCTTCAA CTGCTTTGAC TTTGGGGTGA TTGTGGGGAG TATCTTTGAA 
GTAGTCTGGG CTGCCATCAA GCCAGGAACC TCCTTTGGAA TCAGTGTGCT GCGGGCTCTG 
CGACTGCTGA GGATATTCAA AGTTACCAAG TATTGGAACT CTCTGAGGAA CCTGGTGGTT 
TCCCTCCTCA ATTCCATGAA GTCCATCATC AGCCTTCTCT TCCTGCTTTT CCTCTTCATC 
GTGGTCTTCG CTCTGTTGGG GATGCAGCTG TTTGGGGGAC AGTTCAACTT TCAAGATGAG 
ACTCCAACCA CCAATTTTGA TACCTTCCCA GCTGCCATCC TCACTGTCTT TCAGATCCTG 
ACAGGAGAGG ATTGGAATGC CGTAATGTAT CATGGGATTG AGTCGCAAGG TGGAGTCAGC 
AAAGGCATGT TTTCTTCCTT TTACTTCATC GTCCTGACAC TGTTTGGAAA CTACACCCTG 
CTGAATGTTT TTCTGGCCAT TGCTGTGGAC AACCTTGCCA ATGCCCAGGA GTTGACCAAG 
GATGAAGAGG AGATGGAAGA AGCAGCCAAT CAGAAACTTG CTCTTCAGAA GGCCAAAGAA 
GTAGCTGAAG TCAGCCCCAT GTCTGCTGCC AATATCTCCA TCGCTGCGCA GCAGAACTCG 
GCCAAGGCGC GCTCAGTATG GGAGCAGCGG GCCAGTCAGC TAAGGCTCCA GAATCTGCGT 
GCCAGCTGTG AGGCATTGTA CAGTGAGATG GACCCTGAGG AGCGCCTGCG TTATGCCAGC 
ACGCGCCATG TGAGGCCAGA CATGAAGACA CACATGGACC GACCCCTAGT GGTGGAGCCT 
GGTCGAGATG GCTTGCGGGG ACCCGTTGGG AGCAAGTCAA AGCCTGAAGG CACGGAGGCC 
ACAGAAAGCG CGGACCTACC TCGCAGGCAC CACCGGCACC GTGATAGGGA CAAGACCTCA 
GCCACAGCAC CTGCTGGAGG CGAACAGGAC AGGACAGAAA GCACCGAGAC CGGGGCCCGG 
GAGGAACGTG CGCGCCCTCG TCGAAGTCAC AGCAAGGAGA CTCCAGGGGC TGACACGCAA 
GTGCGCTGTG AGCGCAGTAG ACGTCACCAC CGGCGCGGCT CCCCGGAGGA GGCCACTGAA 
CGGGAGCCTC GGCGCCACCG TGCCCACCGG CATGCACAGG ACTCAAGCAA GGAGGGCACG 
GCGCCGGTGC TTGTACCCAA GGGTGAGCGA CGAGCAAGAC ACCGAGGCCC ACGCACGGGT 
CCACGTGAGG CAGAGAACAA CGAGGAGCCC ACACGCAGGC ACCGTGCAAG GCATAAGGTG 
CCACCCACAC TGCAGCCCCC AGAGAGGGAG GCTGCAGAGA AGGAGAGCAA CGCGGTGGAA 
GGGGATAAGG AAACCCGAAA TCACCAGCCC AAGGAACCTC ACTGTGACCT GGAGGCCATT 
GCAGTTACAG GTGTGGGCCC TCTGCACATG CTGCCCAGCA CCTGTCTCCA GAAAGTGGAC 
GAGCAACCAG AGGATGCAGA CAACCAGCGT AATGTCACCC GGATGGGCAG TCAGCCCTCA 
GATCCCAGCA CCACTGTGCA TGTCCCAGTG ACACTGACAG GCCCTCCTGG GGAGACCCCT 
GTAGTTCCCA GTGGTAACAT GAACCTGGAA GGCCAAGCAG AGGGCAAGAA GGAGGCAGAG 
GCGGATGATG TGCTGAGAAG AGGCCCCAGG CCCATCGTTC CCTACAGCTC CATGTTTTGT 
CTCAGCCCCA CCAACCTGCT TCGTCGCTTC TGCCATTACA TTGTGACCAT GCGGTACTTT 
GAGATGGTAA TTCTTGTGGT CATTGCCTTG AGCAGCATTG CCCTGGCTGC AGAGGATCCT 
GTGCGGACAG ATTCATTCAG GAACAACGCT TTAAAGTACA TGGATTACAT CTTTACAGGA 
GTCTTCACCT TTGAAATGGT CATAAAGATG ATAGACTTGG GCTTGCTGCT GCACCCTGGT 
GCCTACTTCC GGGACCTGTG GAACATTCTG GACTTCATTG TTGTCAGTGG AGCCCTGGTG 
GCATTTGCGT TCTCAGGATC CAAAGGGAAA GACATCAATA CCATCAAGTC TCTGAGAGTC 
CTGCGTGTCC TGAGGCCCCT CAAGACCATC AAGCGGCTGC CTAAGCTCAA GGCTGTCTTT 
GACTGTGTGG TGAACTCCCT GAAGAACGTC TTGAACATCC TGATTGTCTA CATGCTCTTC 
ATGTTCATAT TTGCCGTCAT TGCCGTCCAG CTCTTCAAAG GGAAGTTCTT TTACTGTACT 
GATGAATCCA AGGAGCTGGA GAGGGACTGC CGGGGTCAGT ATTTGGATTA TGAGAAGGAA 
GAAGTAGAAG CCCAGCCAAG GCAGTGGAAG AAATATGACT TCCACTATGA CAATGTTCTC 
TGGGCCTTGT TGACGCTGTT CACAGTGTCC ACGGGAGAGG GGTGGCCCAT GGTGCTGAAA 
CACTCTGTGG ATGCCACCTA TGAGGAACAG GGGCCCAGTC CCGGGTTCCG GATGGAGCTC 
TCCATCTTCT ACGTGGTCTA CTTTGTGGTC TTCCCTTTTT TCTTTGTCAA CATCTTTGTG 
GCCTTGATCA TTATCACCTT CCAGGAACAG GGAGATAAGG TGATGTCTGA ATGCAGCTTA 
GAAAAGAATG AGAGGGCTTG CATTGATTTT GCCATCAGTG CCAAGCCCCT GACACGGTAC 
ATGCCTCAAA ACAAACAGTC GTTCCAGTAT AAGACATGGA CATTCGTGGT CTCTCCACCC 
TTTGAGTACT TCATCATGGC TATGATAGCC CTCAACACAG TGGTGCTGAT GATGAAGTTC 
TATGATGCAC CTTATGAGTA CGAGCTGATG CTGAAATGCC TGAACATTGT CTTCACATCC 
ATGTTCTCGA TGGAGTGCAT ACTGAAGATC ATCGCCTTTG GGGTATTGAA CTACTTCAGA 
GATGCCTGGA ACGTCTTTGA CTTTGTCACG GTTTTGGGAA GTATTACTGA TATTTTAGTA 
ACAGAGATAG CGGAAACGAA CAACTTCATC AACCTAAGCT TCCTTCGCCT CTTCCGGGCG 
GCACGGCTGA TCAAGCTGCT TCGCCAGGGC TACACCATCC GCATCCTATT GTGGACCTTC 
GTCCAGTCCT TTAAGGCGCT GCCCTACGTG TGCCTCCTCA TTGCCATGCT GTTCTTCATC 
TACGCCATCA TCGGCATGCA GGTTTTTGGA AACATTGCCC TTGATGATGA CACCAGTATC 
AACCGACACA ACAACTTCCG GACATTTCTG CAAGCCTTAA TGCTATTGTT CAGGAGTGCC 
ACTGGGGAGG CCTGGCATGA GATCATGCTG TCTTGTCTGG GCAACCGGGC CTGTGACCCA 
CATGCCAACG CCAGTGAGTG CGGGAGCGAC TTTGCCTATT TTTATTTTGT CTCCTTCATC 
TTCCTCTGTT CCTTTCTGAT GTTGAACCTC TTTGTTGCTG TAATCATGGA CAATTTTGAG 
TACCTCACTC GGGACTCTTC CATCCTAGGG CCTCACCACT TAGACGAATT CATTCGAGTC 
TGGGCTGAAT ACGACCCAGC TGCGTGTGGG CGCATCAGTT ACAATGACAT GTTTGAGATG 
CTGAAACACA TGTCCCCACC TCTGGGGTTG GGGAAGAAAT GCCCGGCTCG AGTTGCATAC 
AAGCGCCTGG TTCGCATGAA CATGCCCATA TCCAATGAGG ACATGACGGT GCACTTTACG 
TCCACACTGA TGGCCCTCAT CCGGACAGCA CTGGAGATCA AGCTTGCCCC AGCTGACGAG 
ATGACAGTGG GGAAGGTCTA TGCTGCTCTC ATGATATTTG ACTTCTACAA ACAGAACAAA 
ACCACCAGAG ATCAGACTCA CCAAGCTCCC GGAGGCCTGT CCCAGATGGG TCCCGTTTCC 
CTGTTCCACC CTCTGAAGGC CACCCTGGAA CAGACACAGC CCGCTGTGCT TCGAGGAGCT 
CGGGTTTTCC TTCGGCAAAA GAGTGCAACT TCCCTCAGCA ATGGGGGTGC CATACAAACC 
CAGGAAAGTG GCATCAAGGA GTCGCTGTCC TGGGGCACGC AGAGGACCCA AGATGCACTT 
TATGAGGCCA GAGCACCTCT AGAACGTGGC CATTCTGCAG AGATCCCTGT GGGGCAGTCA 
GGAACACTGG CTGTGGATGT CCAGATGCAG AACATGACAC TGAGAGGACC AGATGGGGAG 
CCCCAGCCTG GGCTGGAAAG CCAAGGCAGA GCTGCCTCTA TGCCACGCCT AGCGGCAGAA 
ACACAGCCGG CCCCTAATGC CAGCCCCATG AAGCGCTCCA TCTCCACACT GGCTCCACGC 
CCACATGGGA CTCAGCTTTG CAGCACAGTT CTGGACCGGC CTCCTCCTAG CCAGGCATCA 
CATCACCACC ACCACCGCTG CCACCGGCGC AGAGACAAGA AGCAAAGGTC CCTGGAAAAG 
GGGCCCAGCC TGTCTGTTGA CCCAGAAGGT GCACCAAGCA CTGCTGCAGG ACCTGGTCTG 
CCCCATGGAG AAGGATCCAC CGCCTGCCGG CGGGACCGTA AACAGGAGCG AGGCCGGTCC 
CAGGAGCGGA GGCAGCCCTC ATCTTCCTCT TCAGAGAAGC AGCGCTTCTA TTCCTGTGAC 
CGCTTTGGGA GCCGGGAGCC CCCGCAACTG ATGCCCTCAC TCAGTAGCCA CCCCACATCG 
CCAACAGCGG CGCTAGAGCC AGCACCCCAC CCACAGGGCA GTGGTTCCGT TAATGGGAGC 
CCCTTGATGT CAACATCCGG TGCTAGCACC CCGGGCCGAG GTGGGCGGAG GCAGCTCCCC 
CAGACTCCTC TGACCCCACG CCCCAGCATC ACCTACAAGA CCGCCAATTC CTCGCCTGTC 
CACTTTGCTG AGGGTCAGAG CGGCCTCCCA GCCTTCTCCC CTGGCCGTCT CAGCCGCGGC 
CTTTCTGAAC ACAATGCCCT GCTCCAGAAA GAGCCCCTGA GCCAGCCTCT AGCTCCTGGC 
TCCCGAATTG GCTCTGACCC TTACCTAGGG CAGCGTCTGG ACAGTGAGGC CTCCGCCCAC 
ACCCTGCCTG AGGATACACT CACCTTTGAA GAGGCAGTGG CCACCAACTC TGGCCGCTCC 
TCCAGGACTT CCTATGTGTC CTCCCTCACT TCCCAATCCC ACCCTCTCCG CCGTGTACCC 
AATGGCTATC ACTGCACTTT GGGACTCAGC ACTGGCGTCC GGGCGCGGCA CAGCTACCAC 
CACCCCGATC AGGACCACTG GTGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cacna1b ( NM_001042528.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001035993.1 Mus musculus calcium channel, voltage-dependent, N type, alpha 1B subunit (Cacna1b), transcript variant 1, mRNA.

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CloneID	OMu19812
Clone ID Related Accession (Same CDS sequence)	NM_001042528.2
Accession Version	NM_001042528.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6984bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-27
Organism	Mus musculus(house mouse)
Product	voltage-dependent N-type calcium channel subunit alpha-1B isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AL732525.13, BX294112.6 and AL732546.13. On Oct 18, 2013 this sequence version replaced NM_001042528.1. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer protein (isoform 1). Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF042317.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END##

ATGGTCCGCT TCGGGGACGA GCTAGGCGGC CGCTATGGGG GCACCGGCGG CGGGGAGCGG 
GCTCGGGGTG GCGGGGCCGG CGGGGCGGGT GGCCCGGGCC AGGGGGGTCT GCCGCCGGGC 
CAGCGGGTCC TGTACAAGCA GTCCATTGCG CAGCGCGCAC GGACTATGGC CCTGTACAAC 
CCCATCCCAG TCAAGCAGAA CTGCTTCACC GTCAACCGCT CGCTCTTCGT CTTCAGCGAG 
GACAACGTCG TCCGCAAATA CGCTAAGCGC ATCACCGAAT GGCCGCCCTT CGAATACATG 
ATCCTGGCCA CCATCATCGC CAACTGCATT GTTCTGGCCC TGGAGCAGCA CCTCCCTGAT 
GGGGACAAGA CTCCCATGTC TGAGCGACTA GATGACACGG AGCCTTACTT CATCGGGATC 
TTTTGCTTCG AGGCGGGCAT CAAGATCATA GCCCTGGGCT TTGTTTTCCA CAAGGGCTCC 
TACCTTCGGA ACGGCTGGAA TGTCATGGAC TTCGTGGTGG TCCTCACGGG GATTCTCGCC 
ACAGCTGGAA CTGACTTTGA CCTGCGCACA CTGAGGGCTG TGCGTGTGCT TAGGCCCCTG 
AAGCTGGTGT CTGGAATTCC AAGCTTGCAG GTGGTGCTTA AGTCCATCAT GAAGGCCATG 
GTCCCGCTGC TGCAGATTGG GCTGCTGCTC TTCTTTGCCA TCCTCATGTT TGCCATCATC 
GGCCTCGAAT TCTATATGGG CAAATTCCAT AAGGCCTGTT TCCCCAACAG CACAGATACA 
GAGCCTGTGG GTGACTTTCC CTGTGGCAAA GATCCCCCTG CTCGTCAGTG TGATGGTGAC 
ACCGAATGCC GGGAGTACTG GCCAGGACCC AACTTTGGCA TCACCAATTT TGACAACATC 
CTGTTTGCCA TCTTGACAGT GTTCCAGTGT ATCACCATGG AGGGCTGGAC TGACATCCTC 
TACAATACAA ATGATGCGGC TGGCAACACA TGGAACTGGT TGTACTTCAT CCCCCTCATC 
ATCATTGGCT CCTTCTTCAT GCTCAACCTG GTGCTGGGTG TGCTTTCCGG AGAGTTTGCC 
AAGGAGCGGG AGCGAGTCGA GAACCGCCGC GCCTTCCTGA AGCTCCGCAG GCAGCAGCAG 
ATTGAGCGAG AGCTGAATGG GTACTTGGAG TGGATCTTCA AGGCAGAGGA AGTCATGTTG 
GCAGAGGAGG ACAAGAATGC AGAAGAGAAA TCCCCTTTGG ATGTGTTGAA GAGAGCTGCC 
ACCAAGAAGA GCCGAAATGA CCTCATCCAT GCAGAAGAGG GGGAGGACCG GTTTGTAGAC 
CTCTGTGCAG TTGGGTCTCC ATTTGCTCGT GCCAGCCTCA AGAGTGGGAA GACGGAGAGC 
TCATCGTACT TCCGGAGAAA GGAGAAGATG TTCCGGTTCT TTATCCGGCG TATGGTGAAA 
GCACAGAGCT TCTACTGGGT GGTACTGTGT GTGGTGGCCC TGAACACACT GTGTGTGGCC 
ATGGTGCACT ATAATCAGCC TCAGCGGCTT ACCACTGCAC TGTACTTTGC AGAGTTTGTT 
TTCCTGGGTC TCTTCCTCAC AGAGATGTCC CTGAAGATGT ATGGCCTAGG GCCCAGAAGT 
TACTTCAGGT CTTCCTTCAA CTGCTTTGAC TTTGGGGTGA TTGTGGGGAG TATCTTTGAA 
GTAGTCTGGG CTGCCATCAA GCCAGGAACC TCCTTTGGAA TCAGTGTGCT GCGGGCTCTG 
CGACTGCTGA GGATATTCAA AGTTACCAAG TATTGGAACT CTCTGAGGAA CCTGGTGGTT 
TCCCTCCTCA ATTCCATGAA GTCCATCATC AGCCTTCTCT TCCTGCTTTT CCTCTTCATC 
GTGGTCTTCG CTCTGTTGGG GATGCAGCTG TTTGGGGGAC AGTTCAACTT TCAAGATGAG 
ACTCCAACCA CCAATTTTGA TACCTTCCCA GCTGCCATCC TCACTGTCTT TCAGATCCTG 
ACAGGAGAGG ATTGGAATGC CGTAATGTAT CATGGGATTG AGTCGCAAGG TGGAGTCAGC 
AAAGGCATGT TTTCTTCCTT TTACTTCATC GTCCTGACAC TGTTTGGAAA CTACACCCTG 
CTGAATGTTT TTCTGGCCAT TGCTGTGGAC AACCTTGCCA ATGCCCAGGA GTTGACCAAG 
GATGAAGAGG AGATGGAAGA AGCAGCCAAT CAGAAACTTG CTCTTCAGAA GGCCAAAGAA 
GTAGCTGAAG TCAGCCCCAT GTCTGCTGCC AATATCTCCA TCGCTGCCAG GCAGCAGAAC 
TCGGCCAAGG CGCGCTCAGT ATGGGAGCAG CGGGCCAGTC AGCTAAGGCT CCAGAATCTG 
CGTGCCAGCT GTGAGGCATT GTACAGTGAG ATGGACCCTG AGGAGCGCCT GCGTTATGCC 
AGCACGCGCC ATGTGAGGCC AGACATGAAG ACACACATGG ACCGACCCCT AGTGGTGGAG 
CCTGGTCGAG ATGGCTTGCG GGGACCCGTT GGGAGCAAGT CAAAGCCTGA AGGCACGGAG 
GCCACAGAAA GCGCGGACCT ACCTCGCAGG CACCACCGGC ACCGTGATAG GGACAAGACC 
TCAGCCACAG CACCTGCTGG AGGCGAACAG GACAGGACAG AAAGCACCGA GACCGGGGCC 
CGGGAGGAAC GTGCGCGCCC TCGTCGAAGT CACAGCAAGG AGACTCCAGG GGCTGACACG 
CAAGTGCGCT GTGAGCGCAG TAGACGTCAC CACCGGCGCG GCTCCCCGGA GGAGGCCACT 
GAACGGGAGC CTCGGCGCCA CCGTGCCCAC CGGCATGCAC AGGACTCAAG CAAGGAGGGC 
ACGGCGCCGG TGCTTGTACC CAAGGGTGAG CGACGAGCAA GACACCGAGG CCCACGCACG 
GGTCCACGTG AGGCAGAGAA CAACGAGGAG CCCACACGCA GGCACCGTGC AAGGCATAAG 
GTGCCACCCA CACTGCAGCC CCCAGAGAGG GAGGCTGCAG AGAAGGAGAG CAACGCGGTG 
GAAGGGGATA AGGAAACCCG AAATCACCAG CCCAAGGAAC CTCACTGTGA CCTGGAGGCC 
ATTGCAGTTA CAGGTGTGGG CCCTCTGCAC ATGCTGCCCA GCACCTGTCT CCAGAAAGTG 
GACGAGCAAC CAGAGGATGC AGACAACCAG CGTAATGTCA CCCGGATGGG CAGTCAGCCC 
TCAGATCCCA GCACCACTGT GCATGTCCCA GTGACACTGA CAGGCCCTCC TGGGGAGACC 
CCTGTAGTTC CCAGTGGTAA CATGAACCTG GAAGGCCAAG CAGAGGGCAA GAAGGAGGCA 
GAGGCGGATG ATGTGCTGAG AAGAGGCCCC AGGCCCATCG TTCCCTACAG CTCCATGTTT 
TGTCTCAGCC CCACCAACCT GCTTCGTCGC TTCTGCCATT ACATTGTGAC CATGCGGTAC 
TTTGAGATGG TAATTCTTGT GGTCATTGCC TTGAGCAGCA TTGCCCTGGC TGCAGAGGAT 
CCTGTGCGGA CAGATTCATT CAGGAACAAC GCTTTAAAGT ACATGGATTA CATCTTTACA 
GGAGTCTTCA CCTTTGAAAT GGTCATAAAG ATGATAGACT TGGGCTTGCT GCTGCACCCT 
GGTGCCTACT TCCGGGACCT GTGGAACATT CTGGACTTCA TTGTTGTCAG TGGAGCCCTG 
GTGGCATTTG CGTTCTCGAG CTTCATGGGA GGATCCAAAG GGAAAGACAT CAATACCATC 
AAGTCTCTGA GAGTCCTGCG TGTCCTGAGG CCCCTCAAGA CCATCAAGCG GCTGCCTAAG 
CTCAAGGCTG TCTTTGACTG TGTGGTGAAC TCCCTGAAGA ACGTCTTGAA CATCCTGATT 
GTCTACATGC TCTTCATGTT CATATTTGCC GTCATTGCCG TCCAGCTCTT CAAAGGGAAG 
TTCTTTTACT GTACTGATGA ATCCAAGGAG CTGGAGAGGG ACTGCCGGGG TCAGTATTTG 
GATTATGAGA AGGAAGAAGT AGAAGCCCAG CCAAGGCAGT GGAAGAAATA TGACTTCCAC 
TATGACAATG TTCTCTGGGC CTTGTTGACG CTGTTCACAG TGTCCACGGG AGAGGGGTGG 
CCCATGGTGC TGAAACACTC TGTGGATGCC ACCTATGAGG AACAGGGGCC CAGTCCCGGG 
TTCCGGATGG AGCTCTCCAT CTTCTACGTG GTCTACTTTG TGGTCTTCCC TTTTTTCTTT 
GTCAACATCT TTGTGGCCTT GATCATTATC ACCTTCCAGG AACAGGGAGA TAAGGTGATG 
TCTGAATGCA GCTTAGAAAA GAATGAGAGG GCTTGCATTG ATTTTGCCAT CAGTGCCAAG 
CCCCTGACAC GGTACATGCC TCAAAACAAA CAGTCGTTCC AGTATAAGAC ATGGACATTC 
GTGGTCTCTC CACCCTTTGA GTACTTCATC ATGGCTATGA TAGCCCTCAA CACAGTGGTG 
CTGATGATGA AGTTCTATGA TGCACCTTAT GAGTACGAGC TGATGCTGAA ATGCCTGAAC 
ATTGTCTTCA CATCCATGTT CTCGATGGAG TGCATACTGA AGATCATCGC CTTTGGGGTA 
TTGAACTACT TCAGAGATGC CTGGAACGTC TTTGACTTTG TCACGGTTTT GGGAAGTATT 
ACTGATATTT TAGTAACAGA GATAGCGAAC AACTTCATCA ACCTAAGCTT CCTTCGCCTC 
TTCCGGGCGG CACGGCTGAT CAAGCTGCTT CGCCAGGGCT ACACCATCCG CATCCTATTG 
TGGACCTTCG TCCAGTCCTT TAAGGCGCTG CCCTACGTGT GCCTCCTCAT TGCCATGCTG 
TTCTTCATCT ACGCCATCAT CGGCATGCAG GTTTTTGGAA ACATTGCCCT TGATGATGAC 
ACCAGTATCA ACCGACACAA CAACTTCCGG ACATTTCTGC AAGCCTTAAT GCTATTGTTC 
AGGAGTGCCA CTGGGGAGGC CTGGCATGAG ATCATGCTGT CTTGTCTGGG CAACCGGGCC 
TGTGACCCAC ATGCCAACGC CAGTGAGTGC GGGAGCGACT TTGCCTATTT TTATTTTGTC 
TCCTTCATCT TCCTCTGTTC CTTTCTGATG TTGAACCTCT TTGTTGCTGT AATCATGGAC 
AATTTTGAGT ACCTCACTCG GGACTCTTCC ATCCTAGGGC CTCACCACTT AGACGAATTC 
ATTCGAGTCT GGGCTGAATA CGACCCAGCT GCGTGTGGGC GCATCAGTTA CAATGACATG 
TTTGAGATGC TGAAACACAT GTCCCCACCT CTGGGGTTGG GGAAGAAATG CCCGGCTCGA 
GTTGCATACA AGCGCCTGGT TCGCATGAAC ATGCCCATAT CCAATGAGGA CATGACGGTG 
CACTTTACGT CCACACTGAT GGCCCTCATC CGGACAGCAC TGGAGATCAA GCTTGCCCCA 
GCGGGGACGA AGCAGCACCA GTGTGATGCT GAGCTGAGAA AAGAGATCTC TTCTGTGTGG 
GCTAATCTGC CCCAGAAGAC TCTGGACTTA CTGGTACCAC CCCACAAACC TGACGAGATG 
ACAGTGGGGA AGGTCTATGC TGCTCTCATG ATATTTGACT TCTACAAACA GAACAAAACC 
ACCAGAGATC AGACTCACCA AGCTCCCGGA GGCCTGTCCC AGATGGGTCC CGTTTCCCTG 
TTCCACCCTC TGAAGGCCAC CCTGGAACAG ACACAGCCCG CTGTGCTTCG AGGAGCTCGG 
GTTTTCCTTC GGCAAAAGAG TGCAACTTCC CTCAGCAATG GGGGTGCCAT ACAAACCCAG 
GAAAGTGGCA TCAAGGAGTC GCTGTCCTGG GGCACGCAGA GGACCCAAGA TGCACTTTAT 
GAGGCCAGAG CACCTCTAGA ACGTGGCCAT TCTGCAGAGA TCCCTGTGGG GCAGTCAGGA 
ACACTGGCTG TGGATGTCCA GATGCAGAAC ATGACACTGA GAGGACCAGA TGGGGAGCCC 
CAGCCTGGGC TGGAAAGCCA AGGCAGAGCT GCCTCTATGC CACGCCTAGC GGCAGAAACA 
CAGCCGGCCC CTAATGCCAG CCCCATGAAG CGCTCCATCT CCACACTGGC TCCACGCCCA 
CATGGGACTC AGCTTTGCAG CACAGTTCTG GACCGGCCTC CTCCTAGCCA GGCATCACAT 
CACCACCACC ACCGCTGCCA CCGGCGCAGA GACAAGAAGC AAAGGTCCCT GGAAAAGGGG 
CCCAGCCTGT CTGTTGACCC AGAAGGTGCA CCAAGCACTG CTGCAGGACC TGGTCTGCCC 
CATGGAGAAG GATCCACCGC CTGCCGGCGG GACCGTAAAC AGGAGCGAGG CCGGTCCCAG 
GAGCGGAGGC AGCCCTCATC TTCCTCTTCA GAGAAGCAGC GCTTCTATTC CTGTGACCGC 
TTTGGGAGCC GGGAGCCCCC GCAACTGATG CCCTCACTCA GTAGCCACCC CACATCGCCA 
ACAGCGGCGC TAGAGCCAGC ACCCCACCCA CAGGGCAGTG GTTCCGTTAA TGGGAGCCCC 
TTGATGTCAA CATCCGGTGC TAGCACCCCG GGCCGAGGTG GGCGGAGGCA GCTCCCCCAG 
ACTCCTCTGA CCCCACGCCC CAGCATCACC TACAAGACCG CCAATTCCTC GCCTGTCCAC 
TTTGCTGAGG GTCAGAGCGG CCTCCCAGCC TTCTCCCCTG GCCGTCTCAG CCGCGGCCTT 
TCTGAACACA ATGCCCTGCT CCAGAAAGAG CCCCTGAGCC AGCCTCTAGC TCCTGGCTCC 
CGAATTGGCT CTGACCCTTA CCTAGGGCAG CGTCTGGACA GTGAGGCCTC CGCCCACACC 
CTGCCTGAGG ATACACTCAC CTTTGAAGAG GCAGTGGCCA CCAACTCTGG CCGCTCCTCC 
AGGACTTCCT ATGTGTCCTC CCTCACTTCC CAATCCCACC CTCTCCGCCG TGTACCCAAT 
GGCTATCACT GCACTTTGGG ACTCAGCACT GGCGTCCGGG CGCGGCACAG CTACCACCAC 
CCCGATCAGG ACCACTGGTG CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001035993.1
CDS	106..7089
Translation	MVRFGDELGGRYGGTGGGERARGGGAGGAGGPGQGGLPPGQRVLYKQSIAQRARTMALYNPIPVKQNCFTVNRSLFVFSEDNVVRKYAKRITEWPPFEYMILATIIANCIVLALEQHLPDGDKTPMSERLDDTEPYFIGIFCFEAGIKIIALGFVFHKGSYLRNGWNVMDFVVVLTGILATAGTDFDLRTLRAVRVLRPLKLVSGIPSLQVVLKSIMKAMVPLLQIGLLLFFAILMFAIIGLEFYMGKFHKACFPNSTDTEPVGDFPCGKDPPARQCDGDTECREYWPGPNFGITNFDNILFAILTVFQCITMEGWTDILYNTNDAAGNTWNWLYFIPLIIIGSFFMLNLVLGVLSGEFAKERERVENRRAFLKLRRQQQIERELNGYLEWIFKAEEVMLAEEDKNAEEKSPLDVLKRAATKKSRNDLIHAEEGEDRFVDLCAVGSPFARASLKSGKTESSSYFRRKEKMFRFFIRRMVKAQSFYWVVLCVVALNTLCVAMVHYNQPQRLTTALYFAEFVFLGLFLTEMSLKMYGLGPRSYFRSSFNCFDFGVIVGSIFEVVWAAIKPGTSFGISVLRALRLLRIFKVTKYWNSLRNLVVSLLNSMKSIISLLFLLFLFIVVFALLGMQLFGGQFNFQDETPTTNFDTFPAAILTVFQILTGEDWNAVMYHGIESQGGVSKGMFSSFYFIVLTLFGNYTLLNVFLAIAVDNLANAQELTKDEEEMEEAANQKLALQKAKEVAEVSPMSAANISIAARQQNSAKARSVWEQRASQLRLQNLRASCEALYSEMDPEERLRYASTRHVRPDMKTHMDRPLVVEPGRDGLRGPVGSKSKPEGTEATESADLPRRHHRHRDRDKTSATAPAGGEQDRTESTETGAREERARPRRSHSKETPGADTQVRCERSRRHHRRGSPEEATEREPRRHRAHRHAQDSSKEGTAPVLVPKGERRARHRGPRTGPREAENNEEPTRRHRARHKVPPTLQPPEREAAEKESNAVEGDKETRNHQPKEPHCDLEAIAVTGVGPLHMLPSTCLQKVDEQPEDADNQRNVTRMGSQPSDPSTTVHVPVTLTGPPGETPVVPSGNMNLEGQAEGKKEAEADDVLRRGPRPIVPYSSMFCLSPTNLLRRFCHYIVTMRYFEMVILVVIALSSIALAAEDPVRTDSFRNNALKYMDYIFTGVFTFEMVIKMIDLGLLLHPGAYFRDLWNILDFIVVSGALVAFAFSSFMGGSKGKDINTIKSLRVLRVLRPLKTIKRLPKLKAVFDCVVNSLKNVLNILIVYMLFMFIFAVIAVQLFKGKFFYCTDESKELERDCRGQYLDYEKEEVEAQPRQWKKYDFHYDNVLWALLTLFTVSTGEGWPMVLKHSVDATYEEQGPSPGFRMELSIFYVVYFVVFPFFFVNIFVALIIITFQEQGDKVMSECSLEKNERACIDFAISAKPLTRYMPQNKQSFQYKTWTFVVSPPFEYFIMAMIALNTVVLMMKFYDAPYEYELMLKCLNIVFTSMFSMECILKIIAFGVLNYFRDAWNVFDFVTVLGSITDILVTEIANNFINLSFLRLFRAARLIKLLRQGYTIRILLWTFVQSFKALPYVCLLIAMLFFIYAIIGMQVFGNIALDDDTSINRHNNFRTFLQALMLLFRSATGEAWHEIMLSCLGNRACDPHANASECGSDFAYFYFVSFIFLCSFLMLNLFVAVIMDNFEYLTRDSSILGPHHLDEFIRVWAEYDPAACGRISYNDMFEMLKHMSPPLGLGKKCPARVAYKRLVRMNMPISNEDMTVHFTSTLMALIRTALEIKLAPAGTKQHQCDAELRKEISSVWANLPQKTLDLLVPPHKPDEMTVGKVYAALMIFDFYKQNKTTRDQTHQAPGGLSQMGPVSLFHPLKATLEQTQPAVLRGARVFLRQKSATSLSNGGAIQTQESGIKESLSWGTQRTQDALYEARAPLERGHSAEIPVGQSGTLAVDVQMQNMTLRGPDGEPQPGLESQGRAASMPRLAAETQPAPNASPMKRSISTLAPRPHGTQLCSTVLDRPPPSQASHHHHHRCHRRRDKKQRSLEKGPSLSVDPEGAPSTAAGPGLPHGEGSTACRRDRKQERGRSQERRQPSSSSSEKQRFYSCDRFGSREPPQLMPSLSSHPTSPTAALEPAPHPQGSGSVNGSPLMSTSGASTPGRGGRRQLPQTPLTPRPSITYKTANSSPVHFAEGQSGLPAFSPGRLSRGLSEHNALLQKEPLSQPLAPGSRIGSDPYLGQRLDSEASAHTLPEDTLTFEEAVATNSGRSSRTSYVSSLTSQSHPLRRVPNGYHCTLGLSTGVRARHSYHHPDQDHWC

Target ORF information:

RefSeq Version	NM_001042528.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus calcium channel, voltage-dependent, N type, alpha 1B subunit (Cacna1b), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001042528.2

ORF Insert Sequence:

ATGGTCCGCT TCGGGGACGA GCTAGGCGGC CGCTATGGGG GCACCGGCGG CGGGGAGCGG 
GCTCGGGGTG GCGGGGCCGG CGGGGCGGGT GGCCCGGGCC AGGGGGGTCT GCCGCCGGGC 
CAGCGGGTCC TGTACAAGCA GTCCATTGCG CAGCGCGCAC GGACTATGGC CCTGTACAAC 
CCCATCCCAG TCAAGCAGAA CTGCTTCACC GTCAACCGCT CGCTCTTCGT CTTCAGCGAG 
GACAACGTCG TCCGCAAATA CGCTAAGCGC ATCACCGAAT GGCCGCCCTT CGAATACATG 
ATCCTGGCCA CCATCATCGC CAACTGCATT GTTCTGGCCC TGGAGCAGCA CCTCCCTGAT 
GGGGACAAGA CTCCCATGTC TGAGCGACTA GATGACACGG AGCCTTACTT CATCGGGATC 
TTTTGCTTCG AGGCGGGCAT CAAGATCATA GCCCTGGGCT TTGTTTTCCA CAAGGGCTCC 
TACCTTCGGA ACGGCTGGAA TGTCATGGAC TTCGTGGTGG TCCTCACGGG GATTCTCGCC 
ACAGCTGGAA CTGACTTTGA CCTGCGCACA CTGAGGGCTG TGCGTGTGCT TAGGCCCCTG 
AAGCTGGTGT CTGGAATTCC AAGCTTGCAG GTGGTGCTTA AGTCCATCAT GAAGGCCATG 
GTCCCGCTGC TGCAGATTGG GCTGCTGCTC TTCTTTGCCA TCCTCATGTT TGCCATCATC 
GGCCTCGAAT TCTATATGGG CAAATTCCAT AAGGCCTGTT TCCCCAACAG CACAGATACA 
GAGCCTGTGG GTGACTTTCC CTGTGGCAAA GATCCCCCTG CTCGTCAGTG TGATGGTGAC 
ACCGAATGCC GGGAGTACTG GCCAGGACCC AACTTTGGCA TCACCAATTT TGACAACATC 
CTGTTTGCCA TCTTGACAGT GTTCCAGTGT ATCACCATGG AGGGCTGGAC TGACATCCTC 
TACAATACAA ATGATGCGGC TGGCAACACA TGGAACTGGT TGTACTTCAT CCCCCTCATC 
ATCATTGGCT CCTTCTTCAT GCTCAACCTG GTGCTGGGTG TGCTTTCCGG AGAGTTTGCC 
AAGGAGCGGG AGCGAGTCGA GAACCGCCGC GCCTTCCTGA AGCTCCGCAG GCAGCAGCAG 
ATTGAGCGAG AGCTGAATGG GTACTTGGAG TGGATCTTCA AGGCAGAGGA AGTCATGTTG 
GCAGAGGAGG ACAAGAATGC AGAAGAGAAA TCCCCTTTGG ATGTGTTGAA GAGAGCTGCC 
ACCAAGAAGA GCCGAAATGA CCTCATCCAT GCAGAAGAGG GGGAGGACCG GTTTGTAGAC 
CTCTGTGCAG TTGGGTCTCC ATTTGCTCGT GCCAGCCTCA AGAGTGGGAA GACGGAGAGC 
TCATCGTACT TCCGGAGAAA GGAGAAGATG TTCCGGTTCT TTATCCGGCG TATGGTGAAA 
GCACAGAGCT TCTACTGGGT GGTACTGTGT GTGGTGGCCC TGAACACACT GTGTGTGGCC 
ATGGTGCACT ATAATCAGCC TCAGCGGCTT ACCACTGCAC TGTACTTTGC AGAGTTTGTT 
TTCCTGGGTC TCTTCCTCAC AGAGATGTCC CTGAAGATGT ATGGCCTAGG GCCCAGAAGT 
TACTTCAGGT CTTCCTTCAA CTGCTTTGAC TTTGGGGTGA TTGTGGGGAG TATCTTTGAA 
GTAGTCTGGG CTGCCATCAA GCCAGGAACC TCCTTTGGAA TCAGTGTGCT GCGGGCTCTG 
CGACTGCTGA GGATATTCAA AGTTACCAAG TATTGGAACT CTCTGAGGAA CCTGGTGGTT 
TCCCTCCTCA ATTCCATGAA GTCCATCATC AGCCTTCTCT TCCTGCTTTT CCTCTTCATC 
GTGGTCTTCG CTCTGTTGGG GATGCAGCTG TTTGGGGGAC AGTTCAACTT TCAAGATGAG 
ACTCCAACCA CCAATTTTGA TACCTTCCCA GCTGCCATCC TCACTGTCTT TCAGATCCTG 
ACAGGAGAGG ATTGGAATGC CGTAATGTAT CATGGGATTG AGTCGCAAGG TGGAGTCAGC 
AAAGGCATGT TTTCTTCCTT TTACTTCATC GTCCTGACAC TGTTTGGAAA CTACACCCTG 
CTGAATGTTT TTCTGGCCAT TGCTGTGGAC AACCTTGCCA ATGCCCAGGA GTTGACCAAG 
GATGAAGAGG AGATGGAAGA AGCAGCCAAT CAGAAACTTG CTCTTCAGAA GGCCAAAGAA 
GTAGCTGAAG TCAGCCCCAT GTCTGCTGCC AATATCTCCA TCGCTGCCAG GCAGCAGAAC 
TCGGCCAAGG CGCGCTCAGT ATGGGAGCAG CGGGCCAGTC AGCTAAGGCT CCAGAATCTG 
CGTGCCAGCT GTGAGGCATT GTACAGTGAG ATGGACCCTG AGGAGCGCCT GCGTTATGCC 
AGCACGCGCC ATGTGAGGCC AGACATGAAG ACACACATGG ACCGACCCCT AGTGGTGGAG 
CCTGGTCGAG ATGGCTTGCG GGGACCCGTT GGGAGCAAGT CAAAGCCTGA AGGCACGGAG 
GCCACAGAAA GCGCGGACCT ACCTCGCAGG CACCACCGGC ACCGTGATAG GGACAAGACC 
TCAGCCACAG CACCTGCTGG AGGCGAACAG GACAGGACAG AAAGCACCGA GACCGGGGCC 
CGGGAGGAAC GTGCGCGCCC TCGTCGAAGT CACAGCAAGG AGACTCCAGG GGCTGACACG 
CAAGTGCGCT GTGAGCGCAG TAGACGTCAC CACCGGCGCG GCTCCCCGGA GGAGGCCACT 
GAACGGGAGC CTCGGCGCCA CCGTGCCCAC CGGCATGCAC AGGACTCAAG CAAGGAGGGC 
ACGGCGCCGG TGCTTGTACC CAAGGGTGAG CGACGAGCAA GACACCGAGG CCCACGCACG 
GGTCCACGTG AGGCAGAGAA CAACGAGGAG CCCACACGCA GGCACCGTGC AAGGCATAAG 
GTGCCACCCA CACTGCAGCC CCCAGAGAGG GAGGCTGCAG AGAAGGAGAG CAACGCGGTG 
GAAGGGGATA AGGAAACCCG AAATCACCAG CCCAAGGAAC CTCACTGTGA CCTGGAGGCC 
ATTGCAGTTA CAGGTGTGGG CCCTCTGCAC ATGCTGCCCA GCACCTGTCT CCAGAAAGTG 
GACGAGCAAC CAGAGGATGC AGACAACCAG CGTAATGTCA CCCGGATGGG CAGTCAGCCC 
TCAGATCCCA GCACCACTGT GCATGTCCCA GTGACACTGA CAGGCCCTCC TGGGGAGACC 
CCTGTAGTTC CCAGTGGTAA CATGAACCTG GAAGGCCAAG CAGAGGGCAA GAAGGAGGCA 
GAGGCGGATG ATGTGCTGAG AAGAGGCCCC AGGCCCATCG TTCCCTACAG CTCCATGTTT 
TGTCTCAGCC CCACCAACCT GCTTCGTCGC TTCTGCCATT ACATTGTGAC CATGCGGTAC 
TTTGAGATGG TAATTCTTGT GGTCATTGCC TTGAGCAGCA TTGCCCTGGC TGCAGAGGAT 
CCTGTGCGGA CAGATTCATT CAGGAACAAC GCTTTAAAGT ACATGGATTA CATCTTTACA 
GGAGTCTTCA CCTTTGAAAT GGTCATAAAG ATGATAGACT TGGGCTTGCT GCTGCACCCT 
GGTGCCTACT TCCGGGACCT GTGGAACATT CTGGACTTCA TTGTTGTCAG TGGAGCCCTG 
GTGGCATTTG CGTTCTCGAG CTTCATGGGA GGATCCAAAG GGAAAGACAT CAATACCATC 
AAGTCTCTGA GAGTCCTGCG TGTCCTGAGG CCCCTCAAGA CCATCAAGCG GCTGCCTAAG 
CTCAAGGCTG TCTTTGACTG TGTGGTGAAC TCCCTGAAGA ACGTCTTGAA CATCCTGATT 
GTCTACATGC TCTTCATGTT CATATTTGCC GTCATTGCCG TCCAGCTCTT CAAAGGGAAG 
TTCTTTTACT GTACTGATGA ATCCAAGGAG CTGGAGAGGG ACTGCCGGGG TCAGTATTTG 
GATTATGAGA AGGAAGAAGT AGAAGCCCAG CCAAGGCAGT GGAAGAAATA TGACTTCCAC 
TATGACAATG TTCTCTGGGC CTTGTTGACG CTGTTCACAG TGTCCACGGG AGAGGGGTGG 
CCCATGGTGC TGAAACACTC TGTGGATGCC ACCTATGAGG AACAGGGGCC CAGTCCCGGG 
TTCCGGATGG AGCTCTCCAT CTTCTACGTG GTCTACTTTG TGGTCTTCCC TTTTTTCTTT 
GTCAACATCT TTGTGGCCTT GATCATTATC ACCTTCCAGG AACAGGGAGA TAAGGTGATG 
TCTGAATGCA GCTTAGAAAA GAATGAGAGG GCTTGCATTG ATTTTGCCAT CAGTGCCAAG 
CCCCTGACAC GGTACATGCC TCAAAACAAA CAGTCGTTCC AGTATAAGAC ATGGACATTC 
GTGGTCTCTC CACCCTTTGA GTACTTCATC ATGGCTATGA TAGCCCTCAA CACAGTGGTG 
CTGATGATGA AGTTCTATGA TGCACCTTAT GAGTACGAGC TGATGCTGAA ATGCCTGAAC 
ATTGTCTTCA CATCCATGTT CTCGATGGAG TGCATACTGA AGATCATCGC CTTTGGGGTA 
TTGAACTACT TCAGAGATGC CTGGAACGTC TTTGACTTTG TCACGGTTTT GGGAAGTATT 
ACTGATATTT TAGTAACAGA GATAGCGAAC AACTTCATCA ACCTAAGCTT CCTTCGCCTC 
TTCCGGGCGG CACGGCTGAT CAAGCTGCTT CGCCAGGGCT ACACCATCCG CATCCTATTG 
TGGACCTTCG TCCAGTCCTT TAAGGCGCTG CCCTACGTGT GCCTCCTCAT TGCCATGCTG 
TTCTTCATCT ACGCCATCAT CGGCATGCAG GTTTTTGGAA ACATTGCCCT TGATGATGAC 
ACCAGTATCA ACCGACACAA CAACTTCCGG ACATTTCTGC AAGCCTTAAT GCTATTGTTC 
AGGAGTGCCA CTGGGGAGGC CTGGCATGAG ATCATGCTGT CTTGTCTGGG CAACCGGGCC 
TGTGACCCAC ATGCCAACGC CAGTGAGTGC GGGAGCGACT TTGCCTATTT TTATTTTGTC 
TCCTTCATCT TCCTCTGTTC CTTTCTGATG TTGAACCTCT TTGTTGCTGT AATCATGGAC 
AATTTTGAGT ACCTCACTCG GGACTCTTCC ATCCTAGGGC CTCACCACTT AGACGAATTC 
ATTCGAGTCT GGGCTGAATA CGACCCAGCT GCGTGTGGGC GCATCAGTTA CAATGACATG 
TTTGAGATGC TGAAACACAT GTCCCCACCT CTGGGGTTGG GGAAGAAATG CCCGGCTCGA 
GTTGCATACA AGCGCCTGGT TCGCATGAAC ATGCCCATAT CCAATGAGGA CATGACGGTG 
CACTTTACGT CCACACTGAT GGCCCTCATC CGGACAGCAC TGGAGATCAA GCTTGCCCCA 
GCGGGGACGA AGCAGCACCA GTGTGATGCT GAGCTGAGAA AAGAGATCTC TTCTGTGTGG 
GCTAATCTGC CCCAGAAGAC TCTGGACTTA CTGGTACCAC CCCACAAACC TGACGAGATG 
ACAGTGGGGA AGGTCTATGC TGCTCTCATG ATATTTGACT TCTACAAACA GAACAAAACC 
ACCAGAGATC AGACTCACCA AGCTCCCGGA GGCCTGTCCC AGATGGGTCC CGTTTCCCTG 
TTCCACCCTC TGAAGGCCAC CCTGGAACAG ACACAGCCCG CTGTGCTTCG AGGAGCTCGG 
GTTTTCCTTC GGCAAAAGAG TGCAACTTCC CTCAGCAATG GGGGTGCCAT ACAAACCCAG 
GAAAGTGGCA TCAAGGAGTC GCTGTCCTGG GGCACGCAGA GGACCCAAGA TGCACTTTAT 
GAGGCCAGAG CACCTCTAGA ACGTGGCCAT TCTGCAGAGA TCCCTGTGGG GCAGTCAGGA 
ACACTGGCTG TGGATGTCCA GATGCAGAAC ATGACACTGA GAGGACCAGA TGGGGAGCCC 
CAGCCTGGGC TGGAAAGCCA AGGCAGAGCT GCCTCTATGC CACGCCTAGC GGCAGAAACA 
CAGCCGGCCC CTAATGCCAG CCCCATGAAG CGCTCCATCT CCACACTGGC TCCACGCCCA 
CATGGGACTC AGCTTTGCAG CACAGTTCTG GACCGGCCTC CTCCTAGCCA GGCATCACAT 
CACCACCACC ACCGCTGCCA CCGGCGCAGA GACAAGAAGC AAAGGTCCCT GGAAAAGGGG 
CCCAGCCTGT CTGTTGACCC AGAAGGTGCA CCAAGCACTG CTGCAGGACC TGGTCTGCCC 
CATGGAGAAG GATCCACCGC CTGCCGGCGG GACCGTAAAC AGGAGCGAGG CCGGTCCCAG 
GAGCGGAGGC AGCCCTCATC TTCCTCTTCA GAGAAGCAGC GCTTCTATTC CTGTGACCGC 
TTTGGGAGCC GGGAGCCCCC GCAACTGATG CCCTCACTCA GTAGCCACCC CACATCGCCA 
ACAGCGGCGC TAGAGCCAGC ACCCCACCCA CAGGGCAGTG GTTCCGTTAA TGGGAGCCCC 
TTGATGTCAA CATCCGGTGC TAGCACCCCG GGCCGAGGTG GGCGGAGGCA GCTCCCCCAG 
ACTCCTCTGA CCCCACGCCC CAGCATCACC TACAAGACCG CCAATTCCTC GCCTGTCCAC 
TTTGCTGAGG GTCAGAGCGG CCTCCCAGCC TTCTCCCCTG GCCGTCTCAG CCGCGGCCTT 
TCTGAACACA ATGCCCTGCT CCAGAAAGAG CCCCTGAGCC AGCCTCTAGC TCCTGGCTCC 
CGAATTGGCT CTGACCCTTA CCTAGGGCAG CGTCTGGACA GTGAGGCCTC CGCCCACACC 
CTGCCTGAGG ATACACTCAC CTTTGAAGAG GCAGTGGCCA CCAACTCTGG CCGCTCCTCC 
AGGACTTCCT ATGTGTCCTC CCTCACTTCC CAATCCCACC CTCTCCGCCG TGTACCCAAT 
GGCTATCACT GCACTTTGGG ACTCAGCACT GGCGTCCGGG CGCGGCACAG CTACCACCAC 
CCCGATCAGG ACCACTGGTG CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cacna1b ( XM_006497626.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006497689.1 Mus musculus calcium channel, voltage-dependent, N type, alpha 1B subunit (Cacna1b), transcript variant X1, mRNA.

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CloneID	OMu29257
Clone ID Related Accession (Same CDS sequence)	XM_006497626.3
Accession Version	XM_006497626.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7056bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	voltage-dependent N-type calcium channel subunit alpha-1B isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006497626.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTCCGCT TCGGGGACGA GCTAGGCGGC CGCTATGGGG GCACCGGCGG CGGGGAGCGG 
GCTCGGGGTG GCGGGGCCGG CGGGGCGGGT GGCCCGGGCC AGGGGGGTCT GCCGCCGGGC 
CAGCGGGTCC TGTACAAGCA GTCCATTGCG CAGCGCGCAC GGACTATGGC CCTGTACAAC 
CCCATCCCAG TCAAGCAGAA CTGCTTCACC GTCAACCGCT CGCTCTTCGT CTTCAGCGAG 
GACAACGTCG TCCGCAAATA CGCTAAGCGC ATCACCGAAT GGCCGCCCTT CGAATACATG 
ATCCTGGCCA CCATCATCGC CAACTGCATT GTTCTGGCCC TGGAGCAGCA CCTCCCTGAT 
GGGGACAAGA CTCCCATGTC TGAGCGACTA GATGACACGG AGCCTTACTT CATCGGGATC 
TTTTGCTTCG AGGCGGGCAT CAAGATCATA GCCCTGGGCT TTGTTTTCCA CAAGGGCTCC 
TACCTTCGGA ACGGCTGGAA TGTCATGGAC TTCGTGGTGG TCCTCACGGG GATTCTCGCC 
ACAGCTGGAA CTGACTTTGA CCTGCGCACA CTGAGGGCTG TGCGTGTGCT TAGGCCCCTG 
AAGCTGGTGT CTGGAATTCC AAGCTTGCAG GTGGTGCTTA AGTCCATCAT GAAGGCCATG 
GTCCCGCTGC TGCAGATTGG GCTGCTGCTC TTCTTTGCCA TCCTCATGTT TGCCATCATC 
GGCCTCGAAT TCTATATGGG CAAATTCCAT AAGGCCTGTT TCCCCAACAG CACAGATACA 
GAGCCTGTGG GTGACTTTCC CTGTGGCAAA GATCCCCCTG CTCGTCAGTG TGATGGTGAC 
ACCGAATGCC GGGAGTACTG GCCAGGACCC AACTTTGGCA TCACCAATTT TGACAACATC 
CTGTTTGCCA TCTTGACAGT GTTCCAGTGT ATCACCATGG AGGGCTGGAC TGACATCCTC 
TACAATACAA ATGATGCGGC TGGCAACACA TGGAACTGGT TGTACTTCAT CCCCCTCATC 
ATCATTGGCT CCTTCTTCAT GCTCAACCTG GTGCTGGGTG TGCTTTCCGG AGAGTTTGCC 
AAGGAGCGGG AGCGAGTCGA GAACCGCCGC GCCTTCCTGA AGCTCCGCAG GCAGCAGCAG 
ATTGAGCGAG AGCTGAATGG GTACTTGGAG TGGATCTTCA AGGCAGAGGA AGTCATGTTG 
GCAGAGGAGG ACAAGAATGC AGAAGAGAAA TCCCCTTTGG ATGCAGTGTT GAAGAGAGCT 
GCCACCAAGA AGAGCCGAAA TGACCTCATC CATGCAGAAG AGGGGGAGGA CCGGTTTGTA 
GACCTCTGTG CAGTTGGGTC TCCATTTGCT CGTGCCAGCC TCAAGAGTGG GAAGACGGAG 
AGCTCATCGT ACTTCCGGAG AAAGGAGAAG ATGTTCCGGT TCTTTATCCG GCGTATGGTG 
AAAGCACAGA GCTTCTACTG GGTGGTACTG TGTGTGGTGG CCCTGAACAC ACTGTGTGTG 
GCCATGGTGC ACTATAATCA GCCTCAGCGG CTTACCACTG CACTGTACTT TGCAGAGTTT 
GTTTTCCTGG GTCTCTTCCT CACAGAGATG TCCCTGAAGA TGTATGGCCT AGGGCCCAGA 
AGTTACTTCA GGTCTTCCTT CAACTGCTTT GACTTTGGGG TGATTGTGGG GAGTATCTTT 
GAAGTAGTCT GGGCTGCCAT CAAGCCAGGA ACCTCCTTTG GAATCAGTGT GCTGCGGGCT 
CTGCGACTGC TGAGGATATT CAAAGTTACC AAGTATTGGA ACTCTCTGAG GAACCTGGTG 
GTTTCCCTCC TCAATTCCAT GAAGTCCATC ATCAGCCTTC TCTTCCTGCT TTTCCTCTTC 
ATCGTGGTCT TCGCTCTGTT GGGGATGCAG CTGTTTGGGG GACAGTTCAA CTTTCAAGAT 
GAGACTCCAA CCACCAATTT TGATACCTTC CCAGCTGCCA TCCTCACTGT CTTTCAGATC 
CTGACAGGAG AGGATTGGAA TGCCGTAATG TATCATGGGA TTGAGTCGCA AGGTGGAGTC 
AGCAAAGGCA TGTTTTCTTC CTTTTACTTC ATCGTCCTGA CACTGTTTGG AAACTACACC 
CTGCTGAATG TTTTTCTGGC CATTGCTGTG GACAACCTTG CCAATGCCCA GGAGTTGACC 
AAGGATGAAG AGGAGATGGA AGAAGCAGCC AATCAGAAAC TTGCTCTTCA GAAGGCCAAA 
GAAGTAGCTG AAGTCAGCCC CATGTCTGCT GCCAATATCT CCATCGCTGC TTTTGTAAAG 
CAAACTCGAG GTACTGTATC TCGCAGCTCA TCTGTCTCCA GCGTAAACTC ACCCAGGCAG 
CAGAACTCGG CCAAGGCGCG CTCAGTATGG GAGCAGCGGG CCAGTCAGCT AAGGCTCCAG 
AATCTGCGTG CCAGCTGTGA GGCATTGTAC AGTGAGATGG ACCCTGAGGA GCGCCTGCGT 
TATGCCAGCA CGCGCCATGT GAGGCCAGAC ATGAAGACAC ACATGGACCG ACCCCTAGTG 
GTGGAGCCTG GTCGAGATGG CTTGCGGGGA CCCGTTGGGA GCAAGTCAAA GCCTGAAGGC 
ACGGAGGCCA CAGAAAGCGC GGACCTACCT CGCAGGCACC ACCGGCACCG TGATAGGGAC 
AAGACCTCAG CCACAGCACC TGCTGGAGGC GAACAGGACA GGACAGAAAG CACCGAGACC 
GGGGCCCGGG AGGAACGTGC GCGCCCTCGT CGAAGTCACA GCAAGGAGAC TCCAGGGGCT 
GACACGCAAG TGCGCTGTGA GCGCAGTAGA CGTCACCACC GGCGCGGCTC CCCGGAGGAG 
GCCACTGAAC GGGAGCCTCG GCGCCACCGT GCCCACCGGC ATGCACAGGA CTCAAGCAAG 
GAGGGCACGG CGCCGGTGCT TGTACCCAAG GGTGAGCGAC GAGCAAGACA CCGAGGCCCA 
CGCACGGGTC CACGTGAGGC AGAGAACAAC GAGGAGCCCA CACGCAGGCA CCGTGCAAGG 
CATAAGGTGC CACCCACACT GCAGCCCCCA GAGAGGGAGG CTGCAGAGAA GGAGAGCAAC 
GCGGTGGAAG GGGATAAGGA AACCCGAAAT CACCAGCCCA AGGAACCTCA CTGTGACCTG 
GAGGCCATTG CAGTTACAGG TGTGGGCCCT CTGCACATGC TGCCCAGCAC CTGTCTCCAG 
AAAGTGGACG AGCAACCAGA GGATGCAGAC AACCAGCGTA ATGTCACCCG GATGGGCAGT 
CAGCCCTCAG ATCCCAGCAC CACTGTGCAT GTCCCAGTGA CACTGACAGG CCCTCCTGGG 
GAGACCCCTG TAGTTCCCAG TGGTAACATG AACCTGGAAG GCCAAGCAGA GGGCAAGAAG 
GAGGCAGAGG CGGATGATGT GCTGAGAAGA GGCCCCAGGC CCATCGTTCC CTACAGCTCC 
ATGTTTTGTC TCAGCCCCAC CAACCTGCTT CGTCGCTTCT GCCATTACAT TGTGACCATG 
CGGTACTTTG AGATGGTAAT TCTTGTGGTC ATTGCCTTGA GCAGCATTGC CCTGGCTGCA 
GAGGATCCTG TGCGGACAGA TTCATTCAGG AACAACGCTT TAAAGTACAT GGATTACATC 
TTTACAGGAG TCTTCACCTT TGAAATGGTC ATAAAGATGA TAGACTTGGG CTTGCTGCTG 
CACCCTGGTG CCTACTTCCG GGACCTGTGG AACATTCTGG ACTTCATTGT TGTCAGTGGA 
GCCCTGGTGG CATTTGCGTT CTCGAGCTTC ATGGGAGGAT CCAAAGGGAA AGACATCAAT 
ACCATCAAGT CTCTGAGAGT CCTGCGTGTC CTGAGGCCCC TCAAGACCAT CAAGCGGCTG 
CCTAAGCTCA AGGCTGTCTT TGACTGTGTG GTGAACTCCC TGAAGAACGT CTTGAACATC 
CTGATTGTCT ACATGCTCTT CATGTTCATA TTTGCCGTCA TTGCCGTCCA GCTCTTCAAA 
GGGAAGTTCT TTTACTGTAC TGATGAATCC AAGGAGCTGG AGAGGGACTG CCGGGGTCAG 
TATTTGGATT ATGAGAAGGA AGAAGTAGAA GCCCAGCCAA GGCAGTGGAA GAAATATGAC 
TTCCACTATG ACAATGTTCT CTGGGCCTTG TTGACGCTGT TCACAGTGTC CACGGGAGAG 
GGGTGGCCCA TGGTGCTGAA ACACTCTGTG GATGCCACCT ATGAGGAACA GGGGCCCAGT 
CCCGGGTTCC GGATGGAGCT CTCCATCTTC TACGTGGTCT ACTTTGTGGT CTTCCCTTTT 
TTCTTTGTCA ACATCTTTGT GGCCTTGATC ATTATCACCT TCCAGGAACA GGGAGATAAG 
GTGATGTCTG AATGCAGCTT AGAAAAGAAT GAGAGGGCTT GCATTGATTT TGCCATCAGT 
GCCAAGCCCC TGACACGGTA CATGCCTCAA AACAAACAGT CGTTCCAGTA TAAGACATGG 
ACATTCGTGG TCTCTCCACC CTTTGAGTAC TTCATCATGG CTATGATAGC CCTCAACACA 
GTGGTGCTGA TGATGAAGTT CTATGATGCA CCTTATGAGT ACGAGCTGAT GCTGAAATGC 
CTGAACATTG TCTTCACATC CATGTTCTCG ATGGAGTGCA TACTGAAGAT CATCGCCTTT 
GGGGTATTGA ACTACTTCAG AGATGCCTGG AACGTCTTTG ACTTTGTCAC GGTTTTGGGA 
AGTATTACTG ATATTTTAGT AACAGAGATA GCGGAAACGA ACAACTTCAT CAACCTAAGC 
TTCCTTCGCC TCTTCCGGGC GGCACGGCTG ATCAAGCTGC TTCGCCAGGG CTACACCATC 
CGCATCCTAT TGTGGACCTT CGTCCAGTCC TTTAAGGCGC TGCCCTACGT GTGCCTCCTC 
ATTGCCATGC TGTTCTTCAT CTACGCCATC ATCGGCATGC AGGTTTTTGG AAACATTGCC 
CTTGATGATG ACACCAGTAT CAACCGACAC AACAACTTCC GGACATTTCT GCAAGCCTTA 
ATGCTATTGT TCAGGAGTGC CACTGGGGAG GCCTGGCATG AGATCATGCT GTCTTGTCTG 
GGCAACCGGG CCTGTGACCC ACATGCCAAC GCCAGTGAGT GCGGGAGCGA CTTTGCCTAT 
TTTTATTTTG TCTCCTTCAT CTTCCTCTGT TCCTTTCTGA TGTTGAACCT CTTTGTTGCT 
GTAATCATGG ACAATTTTGA GTACCTCACT CGGGACTCTT CCATCCTAGG GCCTCACCAC 
TTAGACGAAT TCATTCGAGT CTGGGCTGAA TACGACCCAG CTGCGTGTGG GCGCATCAGT 
TACAATGACA TGTTTGAGAT GCTGAAACAC ATGTCCCCAC CTCTGGGGTT GGGGAAGAAA 
TGCCCGGCTC GAGTTGCATA CAAGCGCCTG GTTCGCATGA ACATGCCCAT ATCCAATGAG 
GACATGACGG TGCACTTTAC GTCCACACTG ATGGCCCTCA TCCGGACAGC ACTGGAGATC 
AAGCTTGCCC CAGCGGGGAC GAAGCAGCAC CAGTGTGATG CTGAGCTGAG AAAAGAGATC 
TCTTCTGTGT GGGCTAATCT GCCCCAGAAG ACTCTGGACT TACTGGTACC ACCCCACAAA 
CCTGACGAGA TGACAGTGGG GAAGGTCTAT GCTGCTCTCA TGATATTTGA CTTCTACAAA 
CAGAACAAAA CCACCAGAGA TCAGACTCAC CAAGCTCCCG GAGGCCTGTC CCAGATGGGT 
CCCGTTTCCC TGTTCCACCC TCTGAAGGCC ACCCTGGAAC AGACACAGCC CGCTGTGCTT 
CGAGGAGCTC GGGTTTTCCT TCGGCAAAAG AGTGCAACTT CCCTCAGCAA TGGGGGTGCC 
ATACAAACCC AGGAAAGTGG CATCAAGGAG TCGCTGTCCT GGGGCACGCA GAGGACCCAA 
GATGCACTTT ATGAGGCCAG AGCACCTCTA GAACGTGGCC ATTCTGCAGA GATCCCTGTG 
GGGCAGTCAG GAACACTGGC TGTGGATGTC CAGATGCAGA ACATGACACT GAGAGGACCA 
GATGGGGAGC CCCAGCCTGG GCTGGAAAGC CAAGGCAGAG CTGCCTCTAT GCCACGCCTA 
GCGGCAGAAA CACAGCCGGC CCCTAATGCC AGCCCCATGA AGCGCTCCAT CTCCACACTG 
GCTCCACGCC CACATGGGAC TCAGCTTTGC AGCACAGTTC TGGACCGGCC TCCTCCTAGC 
CAGGCATCAC ATCACCACCA CCACCGCTGC CACCGGCGCA GAGACAAGAA GCAAAGGTCC 
CTGGAAAAGG GGCCCAGCCT GTCTGTTGAC CCAGAAGGTG CACCAAGCAC TGCTGCAGGA 
CCTGGTCTGC CCCATGGAGA AGGATCCACC GCCTGCCGGC GGGACCGTAA ACAGGAGCGA 
GGCCGGTCCC AGGAGCGGAG GCAGCCCTCA TCTTCCTCTT CAGAGAAGCA GCGCTTCTAT 
TCCTGTGACC GCTTTGGGAG CCGGGAGCCC CCGCAACTGA TGCCCTCACT CAGTAGCCAC 
CCCACATCGC CAACAGCGGC GCTAGAGCCA GCACCCCACC CACAGGGCAG TGGTTCCGTT 
AATGGGAGCC CCTTGATGTC AACATCCGGT GCTAGCACCC CGGGCCGAGG TGGGCGGAGG 
CAGCTCCCCC AGACTCCTCT GACCCCACGC CCCAGCATCA CCTACAAGAC CGCCAATTCC 
TCGCCTGTCC ACTTTGCTGA GGGTCAGAGC GGCCTCCCAG CCTTCTCCCC TGGCCGTCTC 
AGCCGCGGCC TTTCTGAACA CAATGCCCTG CTCCAGAAAG AGCCCCTGAG CCAGCCTCTA 
GCTCCTGGCT CCCGAATTGG CTCTGACCCT TACCTAGGGC AGCGTCTGGA CAGTGAGGCC 
TCCGCCCACA CCCTGCCTGA GGATACACTC ACCTTTGAAG AGGCAGTGGC CACCAACTCT 
GGCCGCTCCT CCAGGACTTC CTATGTGTCC TCCCTCACTT CCCAATCCCA CCCTCTCCGC 
CGTGTACCCA ATGGCTATCA CTGCACTTTG GGACTCAGCA CTGGCGTCCG GGCGCGGCAC 
AGCTACCACC ACCCCGATCA GGACCACTGG TGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006497689.1
CDS	146..7201
Translation	MVRFGDELGGRYGGTGGGERARGGGAGGAGGPGQGGLPPGQRVLYKQSIAQRARTMALYNPIPVKQNCFTVNRSLFVFSEDNVVRKYAKRITEWPPFEYMILATIIANCIVLALEQHLPDGDKTPMSERLDDTEPYFIGIFCFEAGIKIIALGFVFHKGSYLRNGWNVMDFVVVLTGILATAGTDFDLRTLRAVRVLRPLKLVSGIPSLQVVLKSIMKAMVPLLQIGLLLFFAILMFAIIGLEFYMGKFHKACFPNSTDTEPVGDFPCGKDPPARQCDGDTECREYWPGPNFGITNFDNILFAILTVFQCITMEGWTDILYNTNDAAGNTWNWLYFIPLIIIGSFFMLNLVLGVLSGEFAKERERVENRRAFLKLRRQQQIERELNGYLEWIFKAEEVMLAEEDKNAEEKSPLDAVLKRAATKKSRNDLIHAEEGEDRFVDLCAVGSPFARASLKSGKTESSSYFRRKEKMFRFFIRRMVKAQSFYWVVLCVVALNTLCVAMVHYNQPQRLTTALYFAEFVFLGLFLTEMSLKMYGLGPRSYFRSSFNCFDFGVIVGSIFEVVWAAIKPGTSFGISVLRALRLLRIFKVTKYWNSLRNLVVSLLNSMKSIISLLFLLFLFIVVFALLGMQLFGGQFNFQDETPTTNFDTFPAAILTVFQILTGEDWNAVMYHGIESQGGVSKGMFSSFYFIVLTLFGNYTLLNVFLAIAVDNLANAQELTKDEEEMEEAANQKLALQKAKEVAEVSPMSAANISIAAFVKQTRGTVSRSSSVSSVNSPRQQNSAKARSVWEQRASQLRLQNLRASCEALYSEMDPEERLRYASTRHVRPDMKTHMDRPLVVEPGRDGLRGPVGSKSKPEGTEATESADLPRRHHRHRDRDKTSATAPAGGEQDRTESTETGAREERARPRRSHSKETPGADTQVRCERSRRHHRRGSPEEATEREPRRHRAHRHAQDSSKEGTAPVLVPKGERRARHRGPRTGPREAENNEEPTRRHRARHKVPPTLQPPEREAAEKESNAVEGDKETRNHQPKEPHCDLEAIAVTGVGPLHMLPSTCLQKVDEQPEDADNQRNVTRMGSQPSDPSTTVHVPVTLTGPPGETPVVPSGNMNLEGQAEGKKEAEADDVLRRGPRPIVPYSSMFCLSPTNLLRRFCHYIVTMRYFEMVILVVIALSSIALAAEDPVRTDSFRNNALKYMDYIFTGVFTFEMVIKMIDLGLLLHPGAYFRDLWNILDFIVVSGALVAFAFSSFMGGSKGKDINTIKSLRVLRVLRPLKTIKRLPKLKAVFDCVVNSLKNVLNILIVYMLFMFIFAVIAVQLFKGKFFYCTDESKELERDCRGQYLDYEKEEVEAQPRQWKKYDFHYDNVLWALLTLFTVSTGEGWPMVLKHSVDATYEEQGPSPGFRMELSIFYVVYFVVFPFFFVNIFVALIIITFQEQGDKVMSECSLEKNERACIDFAISAKPLTRYMPQNKQSFQYKTWTFVVSPPFEYFIMAMIALNTVVLMMKFYDAPYEYELMLKCLNIVFTSMFSMECILKIIAFGVLNYFRDAWNVFDFVTVLGSITDILVTEIAETNNFINLSFLRLFRAARLIKLLRQGYTIRILLWTFVQSFKALPYVCLLIAMLFFIYAIIGMQVFGNIALDDDTSINRHNNFRTFLQALMLLFRSATGEAWHEIMLSCLGNRACDPHANASECGSDFAYFYFVSFIFLCSFLMLNLFVAVIMDNFEYLTRDSSILGPHHLDEFIRVWAEYDPAACGRISYNDMFEMLKHMSPPLGLGKKCPARVAYKRLVRMNMPISNEDMTVHFTSTLMALIRTALEIKLAPAGTKQHQCDAELRKEISSVWANLPQKTLDLLVPPHKPDEMTVGKVYAALMIFDFYKQNKTTRDQTHQAPGGLSQMGPVSLFHPLKATLEQTQPAVLRGARVFLRQKSATSLSNGGAIQTQESGIKESLSWGTQRTQDALYEARAPLERGHSAEIPVGQSGTLAVDVQMQNMTLRGPDGEPQPGLESQGRAASMPRLAAETQPAPNASPMKRSISTLAPRPHGTQLCSTVLDRPPPSQASHHHHHRCHRRRDKKQRSLEKGPSLSVDPEGAPSTAAGPGLPHGEGSTACRRDRKQERGRSQERRQPSSSSSEKQRFYSCDRFGSREPPQLMPSLSSHPTSPTAALEPAPHPQGSGSVNGSPLMSTSGASTPGRGGRRQLPQTPLTPRPSITYKTANSSPVHFAEGQSGLPAFSPGRLSRGLSEHNALLQKEPLSQPLAPGSRIGSDPYLGQRLDSEASAHTLPEDTLTFEEAVATNSGRSSRTSYVSSLTSQSHPLRRVPNGYHCTLGLSTGVRARHSYHHPDQDHWC

Target ORF information:

RefSeq Version	XM_006497626.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus calcium channel, voltage-dependent, N type, alpha 1B subunit (Cacna1b), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006497626.3

ORF Insert Sequence:

ATGGTCCGCT TCGGGGACGA GCTAGGCGGC CGCTATGGGG GCACCGGCGG CGGGGAGCGG 
GCTCGGGGTG GCGGGGCCGG CGGGGCGGGT GGCCCGGGCC AGGGGGGTCT GCCGCCGGGC 
CAGCGGGTCC TGTACAAGCA GTCCATTGCG CAGCGCGCAC GGACTATGGC CCTGTACAAC 
CCCATCCCAG TCAAGCAGAA CTGCTTCACC GTCAACCGCT CGCTCTTCGT CTTCAGCGAG 
GACAACGTCG TCCGCAAATA CGCTAAGCGC ATCACCGAAT GGCCGCCCTT CGAATACATG 
ATCCTGGCCA CCATCATCGC CAACTGCATT GTTCTGGCCC TGGAGCAGCA CCTCCCTGAT 
GGGGACAAGA CTCCCATGTC TGAGCGACTA GATGACACGG AGCCTTACTT CATCGGGATC 
TTTTGCTTCG AGGCGGGCAT CAAGATCATA GCCCTGGGCT TTGTTTTCCA CAAGGGCTCC 
TACCTTCGGA ACGGCTGGAA TGTCATGGAC TTCGTGGTGG TCCTCACGGG GATTCTCGCC 
ACAGCTGGAA CTGACTTTGA CCTGCGCACA CTGAGGGCTG TGCGTGTGCT TAGGCCCCTG 
AAGCTGGTGT CTGGAATTCC AAGCTTGCAG GTGGTGCTTA AGTCCATCAT GAAGGCCATG 
GTCCCGCTGC TGCAGATTGG GCTGCTGCTC TTCTTTGCCA TCCTCATGTT TGCCATCATC 
GGCCTCGAAT TCTATATGGG CAAATTCCAT AAGGCCTGTT TCCCCAACAG CACAGATACA 
GAGCCTGTGG GTGACTTTCC CTGTGGCAAA GATCCCCCTG CTCGTCAGTG TGATGGTGAC 
ACCGAATGCC GGGAGTACTG GCCAGGACCC AACTTTGGCA TCACCAATTT TGACAACATC 
CTGTTTGCCA TCTTGACAGT GTTCCAGTGT ATCACCATGG AGGGCTGGAC TGACATCCTC 
TACAATACAA ATGATGCGGC TGGCAACACA TGGAACTGGT TGTACTTCAT CCCCCTCATC 
ATCATTGGCT CCTTCTTCAT GCTCAACCTG GTGCTGGGTG TGCTTTCCGG AGAGTTTGCC 
AAGGAGCGGG AGCGAGTCGA GAACCGCCGC GCCTTCCTGA AGCTCCGCAG GCAGCAGCAG 
ATTGAGCGAG AGCTGAATGG GTACTTGGAG TGGATCTTCA AGGCAGAGGA AGTCATGTTG 
GCAGAGGAGG ACAAGAATGC AGAAGAGAAA TCCCCTTTGG ATGCAGTGTT GAAGAGAGCT 
GCCACCAAGA AGAGCCGAAA TGACCTCATC CATGCAGAAG AGGGGGAGGA CCGGTTTGTA 
GACCTCTGTG CAGTTGGGTC TCCATTTGCT CGTGCCAGCC TCAAGAGTGG GAAGACGGAG 
AGCTCATCGT ACTTCCGGAG AAAGGAGAAG ATGTTCCGGT TCTTTATCCG GCGTATGGTG 
AAAGCACAGA GCTTCTACTG GGTGGTACTG TGTGTGGTGG CCCTGAACAC ACTGTGTGTG 
GCCATGGTGC ACTATAATCA GCCTCAGCGG CTTACCACTG CACTGTACTT TGCAGAGTTT 
GTTTTCCTGG GTCTCTTCCT CACAGAGATG TCCCTGAAGA TGTATGGCCT AGGGCCCAGA 
AGTTACTTCA GGTCTTCCTT CAACTGCTTT GACTTTGGGG TGATTGTGGG GAGTATCTTT 
GAAGTAGTCT GGGCTGCCAT CAAGCCAGGA ACCTCCTTTG GAATCAGTGT GCTGCGGGCT 
CTGCGACTGC TGAGGATATT CAAAGTTACC AAGTATTGGA ACTCTCTGAG GAACCTGGTG 
GTTTCCCTCC TCAATTCCAT GAAGTCCATC ATCAGCCTTC TCTTCCTGCT TTTCCTCTTC 
ATCGTGGTCT TCGCTCTGTT GGGGATGCAG CTGTTTGGGG GACAGTTCAA CTTTCAAGAT 
GAGACTCCAA CCACCAATTT TGATACCTTC CCAGCTGCCA TCCTCACTGT CTTTCAGATC 
CTGACAGGAG AGGATTGGAA TGCCGTAATG TATCATGGGA TTGAGTCGCA AGGTGGAGTC 
AGCAAAGGCA TGTTTTCTTC CTTTTACTTC ATCGTCCTGA CACTGTTTGG AAACTACACC 
CTGCTGAATG TTTTTCTGGC CATTGCTGTG GACAACCTTG CCAATGCCCA GGAGTTGACC 
AAGGATGAAG AGGAGATGGA AGAAGCAGCC AATCAGAAAC TTGCTCTTCA GAAGGCCAAA 
GAAGTAGCTG AAGTCAGCCC CATGTCTGCT GCCAATATCT CCATCGCTGC TTTTGTAAAG 
CAAACTCGAG GTACTGTATC TCGCAGCTCA TCTGTCTCCA GCGTAAACTC ACCCAGGCAG 
CAGAACTCGG CCAAGGCGCG CTCAGTATGG GAGCAGCGGG CCAGTCAGCT AAGGCTCCAG 
AATCTGCGTG CCAGCTGTGA GGCATTGTAC AGTGAGATGG ACCCTGAGGA GCGCCTGCGT 
TATGCCAGCA CGCGCCATGT GAGGCCAGAC ATGAAGACAC ACATGGACCG ACCCCTAGTG 
GTGGAGCCTG GTCGAGATGG CTTGCGGGGA CCCGTTGGGA GCAAGTCAAA GCCTGAAGGC 
ACGGAGGCCA CAGAAAGCGC GGACCTACCT CGCAGGCACC ACCGGCACCG TGATAGGGAC 
AAGACCTCAG CCACAGCACC TGCTGGAGGC GAACAGGACA GGACAGAAAG CACCGAGACC 
GGGGCCCGGG AGGAACGTGC GCGCCCTCGT CGAAGTCACA GCAAGGAGAC TCCAGGGGCT 
GACACGCAAG TGCGCTGTGA GCGCAGTAGA CGTCACCACC GGCGCGGCTC CCCGGAGGAG 
GCCACTGAAC GGGAGCCTCG GCGCCACCGT GCCCACCGGC ATGCACAGGA CTCAAGCAAG 
GAGGGCACGG CGCCGGTGCT TGTACCCAAG GGTGAGCGAC GAGCAAGACA CCGAGGCCCA 
CGCACGGGTC CACGTGAGGC AGAGAACAAC GAGGAGCCCA CACGCAGGCA CCGTGCAAGG 
CATAAGGTGC CACCCACACT GCAGCCCCCA GAGAGGGAGG CTGCAGAGAA GGAGAGCAAC 
GCGGTGGAAG GGGATAAGGA AACCCGAAAT CACCAGCCCA AGGAACCTCA CTGTGACCTG 
GAGGCCATTG CAGTTACAGG TGTGGGCCCT CTGCACATGC TGCCCAGCAC CTGTCTCCAG 
AAAGTGGACG AGCAACCAGA GGATGCAGAC AACCAGCGTA ATGTCACCCG GATGGGCAGT 
CAGCCCTCAG ATCCCAGCAC CACTGTGCAT GTCCCAGTGA CACTGACAGG CCCTCCTGGG 
GAGACCCCTG TAGTTCCCAG TGGTAACATG AACCTGGAAG GCCAAGCAGA GGGCAAGAAG 
GAGGCAGAGG CGGATGATGT GCTGAGAAGA GGCCCCAGGC CCATCGTTCC CTACAGCTCC 
ATGTTTTGTC TCAGCCCCAC CAACCTGCTT CGTCGCTTCT GCCATTACAT TGTGACCATG 
CGGTACTTTG AGATGGTAAT TCTTGTGGTC ATTGCCTTGA GCAGCATTGC CCTGGCTGCA 
GAGGATCCTG TGCGGACAGA TTCATTCAGG AACAACGCTT TAAAGTACAT GGATTACATC 
TTTACAGGAG TCTTCACCTT TGAAATGGTC ATAAAGATGA TAGACTTGGG CTTGCTGCTG 
CACCCTGGTG CCTACTTCCG GGACCTGTGG AACATTCTGG ACTTCATTGT TGTCAGTGGA 
GCCCTGGTGG CATTTGCGTT CTCGAGCTTC ATGGGAGGAT CCAAAGGGAA AGACATCAAT 
ACCATCAAGT CTCTGAGAGT CCTGCGTGTC CTGAGGCCCC TCAAGACCAT CAAGCGGCTG 
CCTAAGCTCA AGGCTGTCTT TGACTGTGTG GTGAACTCCC TGAAGAACGT CTTGAACATC 
CTGATTGTCT ACATGCTCTT CATGTTCATA TTTGCCGTCA TTGCCGTCCA GCTCTTCAAA 
GGGAAGTTCT TTTACTGTAC TGATGAATCC AAGGAGCTGG AGAGGGACTG CCGGGGTCAG 
TATTTGGATT ATGAGAAGGA AGAAGTAGAA GCCCAGCCAA GGCAGTGGAA GAAATATGAC 
TTCCACTATG ACAATGTTCT CTGGGCCTTG TTGACGCTGT TCACAGTGTC CACGGGAGAG 
GGGTGGCCCA TGGTGCTGAA ACACTCTGTG GATGCCACCT ATGAGGAACA GGGGCCCAGT 
CCCGGGTTCC GGATGGAGCT CTCCATCTTC TACGTGGTCT ACTTTGTGGT CTTCCCTTTT 
TTCTTTGTCA ACATCTTTGT GGCCTTGATC ATTATCACCT TCCAGGAACA GGGAGATAAG 
GTGATGTCTG AATGCAGCTT AGAAAAGAAT GAGAGGGCTT GCATTGATTT TGCCATCAGT 
GCCAAGCCCC TGACACGGTA CATGCCTCAA AACAAACAGT CGTTCCAGTA TAAGACATGG 
ACATTCGTGG TCTCTCCACC CTTTGAGTAC TTCATCATGG CTATGATAGC CCTCAACACA 
GTGGTGCTGA TGATGAAGTT CTATGATGCA CCTTATGAGT ACGAGCTGAT GCTGAAATGC 
CTGAACATTG TCTTCACATC CATGTTCTCG ATGGAGTGCA TACTGAAGAT CATCGCCTTT 
GGGGTATTGA ACTACTTCAG AGATGCCTGG AACGTCTTTG ACTTTGTCAC GGTTTTGGGA 
AGTATTACTG ATATTTTAGT AACAGAGATA GCGGAAACGA ACAACTTCAT CAACCTAAGC 
TTCCTTCGCC TCTTCCGGGC GGCACGGCTG ATCAAGCTGC TTCGCCAGGG CTACACCATC 
CGCATCCTAT TGTGGACCTT CGTCCAGTCC TTTAAGGCGC TGCCCTACGT GTGCCTCCTC 
ATTGCCATGC TGTTCTTCAT CTACGCCATC ATCGGCATGC AGGTTTTTGG AAACATTGCC 
CTTGATGATG ACACCAGTAT CAACCGACAC AACAACTTCC GGACATTTCT GCAAGCCTTA 
ATGCTATTGT TCAGGAGTGC CACTGGGGAG GCCTGGCATG AGATCATGCT GTCTTGTCTG 
GGCAACCGGG CCTGTGACCC ACATGCCAAC GCCAGTGAGT GCGGGAGCGA CTTTGCCTAT 
TTTTATTTTG TCTCCTTCAT CTTCCTCTGT TCCTTTCTGA TGTTGAACCT CTTTGTTGCT 
GTAATCATGG ACAATTTTGA GTACCTCACT CGGGACTCTT CCATCCTAGG GCCTCACCAC 
TTAGACGAAT TCATTCGAGT CTGGGCTGAA TACGACCCAG CTGCGTGTGG GCGCATCAGT 
TACAATGACA TGTTTGAGAT GCTGAAACAC ATGTCCCCAC CTCTGGGGTT GGGGAAGAAA 
TGCCCGGCTC GAGTTGCATA CAAGCGCCTG GTTCGCATGA ACATGCCCAT ATCCAATGAG 
GACATGACGG TGCACTTTAC GTCCACACTG ATGGCCCTCA TCCGGACAGC ACTGGAGATC 
AAGCTTGCCC CAGCGGGGAC GAAGCAGCAC CAGTGTGATG CTGAGCTGAG AAAAGAGATC 
TCTTCTGTGT GGGCTAATCT GCCCCAGAAG ACTCTGGACT TACTGGTACC ACCCCACAAA 
CCTGACGAGA TGACAGTGGG GAAGGTCTAT GCTGCTCTCA TGATATTTGA CTTCTACAAA 
CAGAACAAAA CCACCAGAGA TCAGACTCAC CAAGCTCCCG GAGGCCTGTC CCAGATGGGT 
CCCGTTTCCC TGTTCCACCC TCTGAAGGCC ACCCTGGAAC AGACACAGCC CGCTGTGCTT 
CGAGGAGCTC GGGTTTTCCT TCGGCAAAAG AGTGCAACTT CCCTCAGCAA TGGGGGTGCC 
ATACAAACCC AGGAAAGTGG CATCAAGGAG TCGCTGTCCT GGGGCACGCA GAGGACCCAA 
GATGCACTTT ATGAGGCCAG AGCACCTCTA GAACGTGGCC ATTCTGCAGA GATCCCTGTG 
GGGCAGTCAG GAACACTGGC TGTGGATGTC CAGATGCAGA ACATGACACT GAGAGGACCA 
GATGGGGAGC CCCAGCCTGG GCTGGAAAGC CAAGGCAGAG CTGCCTCTAT GCCACGCCTA 
GCGGCAGAAA CACAGCCGGC CCCTAATGCC AGCCCCATGA AGCGCTCCAT CTCCACACTG 
GCTCCACGCC CACATGGGAC TCAGCTTTGC AGCACAGTTC TGGACCGGCC TCCTCCTAGC 
CAGGCATCAC ATCACCACCA CCACCGCTGC CACCGGCGCA GAGACAAGAA GCAAAGGTCC 
CTGGAAAAGG GGCCCAGCCT GTCTGTTGAC CCAGAAGGTG CACCAAGCAC TGCTGCAGGA 
CCTGGTCTGC CCCATGGAGA AGGATCCACC GCCTGCCGGC GGGACCGTAA ACAGGAGCGA 
GGCCGGTCCC AGGAGCGGAG GCAGCCCTCA TCTTCCTCTT CAGAGAAGCA GCGCTTCTAT 
TCCTGTGACC GCTTTGGGAG CCGGGAGCCC CCGCAACTGA TGCCCTCACT CAGTAGCCAC 
CCCACATCGC CAACAGCGGC GCTAGAGCCA GCACCCCACC CACAGGGCAG TGGTTCCGTT 
AATGGGAGCC CCTTGATGTC AACATCCGGT GCTAGCACCC CGGGCCGAGG TGGGCGGAGG 
CAGCTCCCCC AGACTCCTCT GACCCCACGC CCCAGCATCA CCTACAAGAC CGCCAATTCC 
TCGCCTGTCC ACTTTGCTGA GGGTCAGAGC GGCCTCCCAG CCTTCTCCCC TGGCCGTCTC 
AGCCGCGGCC TTTCTGAACA CAATGCCCTG CTCCAGAAAG AGCCCCTGAG CCAGCCTCTA 
GCTCCTGGCT CCCGAATTGG CTCTGACCCT TACCTAGGGC AGCGTCTGGA CAGTGAGGCC 
TCCGCCCACA CCCTGCCTGA GGATACACTC ACCTTTGAAG AGGCAGTGGC CACCAACTCT 
GGCCGCTCCT CCAGGACTTC CTATGTGTCC TCCCTCACTT CCCAATCCCA CCCTCTCCGC 
CGTGTACCCA ATGGCTATCA CTGCACTTTG GGACTCAGCA CTGGCGTCCG GGCGCGGCAC 
AGCTACCACC ACCCCGATCA GGACCACTGG TGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cacna1b ( XM_006497627.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006497690.1 Mus musculus calcium channel, voltage-dependent, N type, alpha 1B subunit (Cacna1b), transcript variant X2, mRNA.

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CloneID	OMu29258
Clone ID Related Accession (Same CDS sequence)	XM_006497627.3
Accession Version	XM_006497627.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7053bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	voltage-dependent N-type calcium channel subunit alpha-1B isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006497627.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTCCGCT TCGGGGACGA GCTAGGCGGC CGCTATGGGG GCACCGGCGG CGGGGAGCGG 
GCTCGGGGTG GCGGGGCCGG CGGGGCGGGT GGCCCGGGCC AGGGGGGTCT GCCGCCGGGC 
CAGCGGGTCC TGTACAAGCA GTCCATTGCG CAGCGCGCAC GGACTATGGC CCTGTACAAC 
CCCATCCCAG TCAAGCAGAA CTGCTTCACC GTCAACCGCT CGCTCTTCGT CTTCAGCGAG 
GACAACGTCG TCCGCAAATA CGCTAAGCGC ATCACCGAAT GGCCGCCCTT CGAATACATG 
ATCCTGGCCA CCATCATCGC CAACTGCATT GTTCTGGCCC TGGAGCAGCA CCTCCCTGAT 
GGGGACAAGA CTCCCATGTC TGAGCGACTA GATGACACGG AGCCTTACTT CATCGGGATC 
TTTTGCTTCG AGGCGGGCAT CAAGATCATA GCCCTGGGCT TTGTTTTCCA CAAGGGCTCC 
TACCTTCGGA ACGGCTGGAA TGTCATGGAC TTCGTGGTGG TCCTCACGGG GATTCTCGCC 
ACAGCTGGAA CTGACTTTGA CCTGCGCACA CTGAGGGCTG TGCGTGTGCT TAGGCCCCTG 
AAGCTGGTGT CTGGAATTCC AAGCTTGCAG GTGGTGCTTA AGTCCATCAT GAAGGCCATG 
GTCCCGCTGC TGCAGATTGG GCTGCTGCTC TTCTTTGCCA TCCTCATGTT TGCCATCATC 
GGCCTCGAAT TCTATATGGG CAAATTCCAT AAGGCCTGTT TCCCCAACAG CACAGATACA 
GAGCCTGTGG GTGACTTTCC CTGTGGCAAA GATCCCCCTG CTCGTCAGTG TGATGGTGAC 
ACCGAATGCC GGGAGTACTG GCCAGGACCC AACTTTGGCA TCACCAATTT TGACAACATC 
CTGTTTGCCA TCTTGACAGT GTTCCAGTGT ATCACCATGG AGGGCTGGAC TGACATCCTC 
TACAATACAA ATGATGCGGC TGGCAACACA TGGAACTGGT TGTACTTCAT CCCCCTCATC 
ATCATTGGCT CCTTCTTCAT GCTCAACCTG GTGCTGGGTG TGCTTTCCGG AGAGTTTGCC 
AAGGAGCGGG AGCGAGTCGA GAACCGCCGC GCCTTCCTGA AGCTCCGCAG GCAGCAGCAG 
ATTGAGCGAG AGCTGAATGG GTACTTGGAG TGGATCTTCA AGGCAGAGGA AGTCATGTTG 
GCAGAGGAGG ACAAGAATGC AGAAGAGAAA TCCCCTTTGG ATGCAGTGTT GAAGAGAGCT 
GCCACCAAGA AGAGCCGAAA TGACCTCATC CATGCAGAAG AGGGGGAGGA CCGGTTTGTA 
GACCTCTGTG CAGTTGGGTC TCCATTTGCT CGTGCCAGCC TCAAGAGTGG GAAGACGGAG 
AGCTCATCGT ACTTCCGGAG AAAGGAGAAG ATGTTCCGGT TCTTTATCCG GCGTATGGTG 
AAAGCACAGA GCTTCTACTG GGTGGTACTG TGTGTGGTGG CCCTGAACAC ACTGTGTGTG 
GCCATGGTGC ACTATAATCA GCCTCAGCGG CTTACCACTG CACTGTACTT TGCAGAGTTT 
GTTTTCCTGG GTCTCTTCCT CACAGAGATG TCCCTGAAGA TGTATGGCCT AGGGCCCAGA 
AGTTACTTCA GGTCTTCCTT CAACTGCTTT GACTTTGGGG TGATTGTGGG GAGTATCTTT 
GAAGTAGTCT GGGCTGCCAT CAAGCCAGGA ACCTCCTTTG GAATCAGTGT GCTGCGGGCT 
CTGCGACTGC TGAGGATATT CAAAGTTACC AAGTATTGGA ACTCTCTGAG GAACCTGGTG 
GTTTCCCTCC TCAATTCCAT GAAGTCCATC ATCAGCCTTC TCTTCCTGCT TTTCCTCTTC 
ATCGTGGTCT TCGCTCTGTT GGGGATGCAG CTGTTTGGGG GACAGTTCAA CTTTCAAGAT 
GAGACTCCAA CCACCAATTT TGATACCTTC CCAGCTGCCA TCCTCACTGT CTTTCAGATC 
CTGACAGGAG AGGATTGGAA TGCCGTAATG TATCATGGGA TTGAGTCGCA AGGTGGAGTC 
AGCAAAGGCA TGTTTTCTTC CTTTTACTTC ATCGTCCTGA CACTGTTTGG AAACTACACC 
CTGCTGAATG TTTTTCTGGC CATTGCTGTG GACAACCTTG CCAATGCCCA GGAGTTGACC 
AAGGATGAAG AGGAGATGGA AGAAGCAGCC AATCAGAAAC TTGCTCTTCA GAAGGCCAAA 
GAAGTAGCTG AAGTCAGCCC CATGTCTGCT GCCAATATCT CCATCGCTGC TTTTGTAAAG 
CAAACTCGAG GTACTGTATC TCGCAGCTCA TCTGTCTCCA GCGTAAACTC ACCGCAGCAG 
AACTCGGCCA AGGCGCGCTC AGTATGGGAG CAGCGGGCCA GTCAGCTAAG GCTCCAGAAT 
CTGCGTGCCA GCTGTGAGGC ATTGTACAGT GAGATGGACC CTGAGGAGCG CCTGCGTTAT 
GCCAGCACGC GCCATGTGAG GCCAGACATG AAGACACACA TGGACCGACC CCTAGTGGTG 
GAGCCTGGTC GAGATGGCTT GCGGGGACCC GTTGGGAGCA AGTCAAAGCC TGAAGGCACG 
GAGGCCACAG AAAGCGCGGA CCTACCTCGC AGGCACCACC GGCACCGTGA TAGGGACAAG 
ACCTCAGCCA CAGCACCTGC TGGAGGCGAA CAGGACAGGA CAGAAAGCAC CGAGACCGGG 
GCCCGGGAGG AACGTGCGCG CCCTCGTCGA AGTCACAGCA AGGAGACTCC AGGGGCTGAC 
ACGCAAGTGC GCTGTGAGCG CAGTAGACGT CACCACCGGC GCGGCTCCCC GGAGGAGGCC 
ACTGAACGGG AGCCTCGGCG CCACCGTGCC CACCGGCATG CACAGGACTC AAGCAAGGAG 
GGCACGGCGC CGGTGCTTGT ACCCAAGGGT GAGCGACGAG CAAGACACCG AGGCCCACGC 
ACGGGTCCAC GTGAGGCAGA GAACAACGAG GAGCCCACAC GCAGGCACCG TGCAAGGCAT 
AAGGTGCCAC CCACACTGCA GCCCCCAGAG AGGGAGGCTG CAGAGAAGGA GAGCAACGCG 
GTGGAAGGGG ATAAGGAAAC CCGAAATCAC CAGCCCAAGG AACCTCACTG TGACCTGGAG 
GCCATTGCAG TTACAGGTGT GGGCCCTCTG CACATGCTGC CCAGCACCTG TCTCCAGAAA 
GTGGACGAGC AACCAGAGGA TGCAGACAAC CAGCGTAATG TCACCCGGAT GGGCAGTCAG 
CCCTCAGATC CCAGCACCAC TGTGCATGTC CCAGTGACAC TGACAGGCCC TCCTGGGGAG 
ACCCCTGTAG TTCCCAGTGG TAACATGAAC CTGGAAGGCC AAGCAGAGGG CAAGAAGGAG 
GCAGAGGCGG ATGATGTGCT GAGAAGAGGC CCCAGGCCCA TCGTTCCCTA CAGCTCCATG 
TTTTGTCTCA GCCCCACCAA CCTGCTTCGT CGCTTCTGCC ATTACATTGT GACCATGCGG 
TACTTTGAGA TGGTAATTCT TGTGGTCATT GCCTTGAGCA GCATTGCCCT GGCTGCAGAG 
GATCCTGTGC GGACAGATTC ATTCAGGAAC AACGCTTTAA AGTACATGGA TTACATCTTT 
ACAGGAGTCT TCACCTTTGA AATGGTCATA AAGATGATAG ACTTGGGCTT GCTGCTGCAC 
CCTGGTGCCT ACTTCCGGGA CCTGTGGAAC ATTCTGGACT TCATTGTTGT CAGTGGAGCC 
CTGGTGGCAT TTGCGTTCTC GAGCTTCATG GGAGGATCCA AAGGGAAAGA CATCAATACC 
ATCAAGTCTC TGAGAGTCCT GCGTGTCCTG AGGCCCCTCA AGACCATCAA GCGGCTGCCT 
AAGCTCAAGG CTGTCTTTGA CTGTGTGGTG AACTCCCTGA AGAACGTCTT GAACATCCTG 
ATTGTCTACA TGCTCTTCAT GTTCATATTT GCCGTCATTG CCGTCCAGCT CTTCAAAGGG 
AAGTTCTTTT ACTGTACTGA TGAATCCAAG GAGCTGGAGA GGGACTGCCG GGGTCAGTAT 
TTGGATTATG AGAAGGAAGA AGTAGAAGCC CAGCCAAGGC AGTGGAAGAA ATATGACTTC 
CACTATGACA ATGTTCTCTG GGCCTTGTTG ACGCTGTTCA CAGTGTCCAC GGGAGAGGGG 
TGGCCCATGG TGCTGAAACA CTCTGTGGAT GCCACCTATG AGGAACAGGG GCCCAGTCCC 
GGGTTCCGGA TGGAGCTCTC CATCTTCTAC GTGGTCTACT TTGTGGTCTT CCCTTTTTTC 
TTTGTCAACA TCTTTGTGGC CTTGATCATT ATCACCTTCC AGGAACAGGG AGATAAGGTG 
ATGTCTGAAT GCAGCTTAGA AAAGAATGAG AGGGCTTGCA TTGATTTTGC CATCAGTGCC 
AAGCCCCTGA CACGGTACAT GCCTCAAAAC AAACAGTCGT TCCAGTATAA GACATGGACA 
TTCGTGGTCT CTCCACCCTT TGAGTACTTC ATCATGGCTA TGATAGCCCT CAACACAGTG 
GTGCTGATGA TGAAGTTCTA TGATGCACCT TATGAGTACG AGCTGATGCT GAAATGCCTG 
AACATTGTCT TCACATCCAT GTTCTCGATG GAGTGCATAC TGAAGATCAT CGCCTTTGGG 
GTATTGAACT ACTTCAGAGA TGCCTGGAAC GTCTTTGACT TTGTCACGGT TTTGGGAAGT 
ATTACTGATA TTTTAGTAAC AGAGATAGCG GAAACGAACA ACTTCATCAA CCTAAGCTTC 
CTTCGCCTCT TCCGGGCGGC ACGGCTGATC AAGCTGCTTC GCCAGGGCTA CACCATCCGC 
ATCCTATTGT GGACCTTCGT CCAGTCCTTT AAGGCGCTGC CCTACGTGTG CCTCCTCATT 
GCCATGCTGT TCTTCATCTA CGCCATCATC GGCATGCAGG TTTTTGGAAA CATTGCCCTT 
GATGATGACA CCAGTATCAA CCGACACAAC AACTTCCGGA CATTTCTGCA AGCCTTAATG 
CTATTGTTCA GGAGTGCCAC TGGGGAGGCC TGGCATGAGA TCATGCTGTC TTGTCTGGGC 
AACCGGGCCT GTGACCCACA TGCCAACGCC AGTGAGTGCG GGAGCGACTT TGCCTATTTT 
TATTTTGTCT CCTTCATCTT CCTCTGTTCC TTTCTGATGT TGAACCTCTT TGTTGCTGTA 
ATCATGGACA ATTTTGAGTA CCTCACTCGG GACTCTTCCA TCCTAGGGCC TCACCACTTA 
GACGAATTCA TTCGAGTCTG GGCTGAATAC GACCCAGCTG CGTGTGGGCG CATCAGTTAC 
AATGACATGT TTGAGATGCT GAAACACATG TCCCCACCTC TGGGGTTGGG GAAGAAATGC 
CCGGCTCGAG TTGCATACAA GCGCCTGGTT CGCATGAACA TGCCCATATC CAATGAGGAC 
ATGACGGTGC ACTTTACGTC CACACTGATG GCCCTCATCC GGACAGCACT GGAGATCAAG 
CTTGCCCCAG CGGGGACGAA GCAGCACCAG TGTGATGCTG AGCTGAGAAA AGAGATCTCT 
TCTGTGTGGG CTAATCTGCC CCAGAAGACT CTGGACTTAC TGGTACCACC CCACAAACCT 
GACGAGATGA CAGTGGGGAA GGTCTATGCT GCTCTCATGA TATTTGACTT CTACAAACAG 
AACAAAACCA CCAGAGATCA GACTCACCAA GCTCCCGGAG GCCTGTCCCA GATGGGTCCC 
GTTTCCCTGT TCCACCCTCT GAAGGCCACC CTGGAACAGA CACAGCCCGC TGTGCTTCGA 
GGAGCTCGGG TTTTCCTTCG GCAAAAGAGT GCAACTTCCC TCAGCAATGG GGGTGCCATA 
CAAACCCAGG AAAGTGGCAT CAAGGAGTCG CTGTCCTGGG GCACGCAGAG GACCCAAGAT 
GCACTTTATG AGGCCAGAGC ACCTCTAGAA CGTGGCCATT CTGCAGAGAT CCCTGTGGGG 
CAGTCAGGAA CACTGGCTGT GGATGTCCAG ATGCAGAACA TGACACTGAG AGGACCAGAT 
GGGGAGCCCC AGCCTGGGCT GGAAAGCCAA GGCAGAGCTG CCTCTATGCC ACGCCTAGCG 
GCAGAAACAC AGCCGGCCCC TAATGCCAGC CCCATGAAGC GCTCCATCTC CACACTGGCT 
CCACGCCCAC ATGGGACTCA GCTTTGCAGC ACAGTTCTGG ACCGGCCTCC TCCTAGCCAG 
GCATCACATC ACCACCACCA CCGCTGCCAC CGGCGCAGAG ACAAGAAGCA AAGGTCCCTG 
GAAAAGGGGC CCAGCCTGTC TGTTGACCCA GAAGGTGCAC CAAGCACTGC TGCAGGACCT 
GGTCTGCCCC ATGGAGAAGG ATCCACCGCC TGCCGGCGGG ACCGTAAACA GGAGCGAGGC 
CGGTCCCAGG AGCGGAGGCA GCCCTCATCT TCCTCTTCAG AGAAGCAGCG CTTCTATTCC 
TGTGACCGCT TTGGGAGCCG GGAGCCCCCG CAACTGATGC CCTCACTCAG TAGCCACCCC 
ACATCGCCAA CAGCGGCGCT AGAGCCAGCA CCCCACCCAC AGGGCAGTGG TTCCGTTAAT 
GGGAGCCCCT TGATGTCAAC ATCCGGTGCT AGCACCCCGG GCCGAGGTGG GCGGAGGCAG 
CTCCCCCAGA CTCCTCTGAC CCCACGCCCC AGCATCACCT ACAAGACCGC CAATTCCTCG 
CCTGTCCACT TTGCTGAGGG TCAGAGCGGC CTCCCAGCCT TCTCCCCTGG CCGTCTCAGC 
CGCGGCCTTT CTGAACACAA TGCCCTGCTC CAGAAAGAGC CCCTGAGCCA GCCTCTAGCT 
CCTGGCTCCC GAATTGGCTC TGACCCTTAC CTAGGGCAGC GTCTGGACAG TGAGGCCTCC 
GCCCACACCC TGCCTGAGGA TACACTCACC TTTGAAGAGG CAGTGGCCAC CAACTCTGGC 
CGCTCCTCCA GGACTTCCTA TGTGTCCTCC CTCACTTCCC AATCCCACCC TCTCCGCCGT 
GTACCCAATG GCTATCACTG CACTTTGGGA CTCAGCACTG GCGTCCGGGC GCGGCACAGC 
TACCACCACC CCGATCAGGA CCACTGGTGC TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006497690.1
CDS	146..7198
Translation	MVRFGDELGGRYGGTGGGERARGGGAGGAGGPGQGGLPPGQRVLYKQSIAQRARTMALYNPIPVKQNCFTVNRSLFVFSEDNVVRKYAKRITEWPPFEYMILATIIANCIVLALEQHLPDGDKTPMSERLDDTEPYFIGIFCFEAGIKIIALGFVFHKGSYLRNGWNVMDFVVVLTGILATAGTDFDLRTLRAVRVLRPLKLVSGIPSLQVVLKSIMKAMVPLLQIGLLLFFAILMFAIIGLEFYMGKFHKACFPNSTDTEPVGDFPCGKDPPARQCDGDTECREYWPGPNFGITNFDNILFAILTVFQCITMEGWTDILYNTNDAAGNTWNWLYFIPLIIIGSFFMLNLVLGVLSGEFAKERERVENRRAFLKLRRQQQIERELNGYLEWIFKAEEVMLAEEDKNAEEKSPLDAVLKRAATKKSRNDLIHAEEGEDRFVDLCAVGSPFARASLKSGKTESSSYFRRKEKMFRFFIRRMVKAQSFYWVVLCVVALNTLCVAMVHYNQPQRLTTALYFAEFVFLGLFLTEMSLKMYGLGPRSYFRSSFNCFDFGVIVGSIFEVVWAAIKPGTSFGISVLRALRLLRIFKVTKYWNSLRNLVVSLLNSMKSIISLLFLLFLFIVVFALLGMQLFGGQFNFQDETPTTNFDTFPAAILTVFQILTGEDWNAVMYHGIESQGGVSKGMFSSFYFIVLTLFGNYTLLNVFLAIAVDNLANAQELTKDEEEMEEAANQKLALQKAKEVAEVSPMSAANISIAAFVKQTRGTVSRSSSVSSVNSPQQNSAKARSVWEQRASQLRLQNLRASCEALYSEMDPEERLRYASTRHVRPDMKTHMDRPLVVEPGRDGLRGPVGSKSKPEGTEATESADLPRRHHRHRDRDKTSATAPAGGEQDRTESTETGAREERARPRRSHSKETPGADTQVRCERSRRHHRRGSPEEATEREPRRHRAHRHAQDSSKEGTAPVLVPKGERRARHRGPRTGPREAENNEEPTRRHRARHKVPPTLQPPEREAAEKESNAVEGDKETRNHQPKEPHCDLEAIAVTGVGPLHMLPSTCLQKVDEQPEDADNQRNVTRMGSQPSDPSTTVHVPVTLTGPPGETPVVPSGNMNLEGQAEGKKEAEADDVLRRGPRPIVPYSSMFCLSPTNLLRRFCHYIVTMRYFEMVILVVIALSSIALAAEDPVRTDSFRNNALKYMDYIFTGVFTFEMVIKMIDLGLLLHPGAYFRDLWNILDFIVVSGALVAFAFSSFMGGSKGKDINTIKSLRVLRVLRPLKTIKRLPKLKAVFDCVVNSLKNVLNILIVYMLFMFIFAVIAVQLFKGKFFYCTDESKELERDCRGQYLDYEKEEVEAQPRQWKKYDFHYDNVLWALLTLFTVSTGEGWPMVLKHSVDATYEEQGPSPGFRMELSIFYVVYFVVFPFFFVNIFVALIIITFQEQGDKVMSECSLEKNERACIDFAISAKPLTRYMPQNKQSFQYKTWTFVVSPPFEYFIMAMIALNTVVLMMKFYDAPYEYELMLKCLNIVFTSMFSMECILKIIAFGVLNYFRDAWNVFDFVTVLGSITDILVTEIAETNNFINLSFLRLFRAARLIKLLRQGYTIRILLWTFVQSFKALPYVCLLIAMLFFIYAIIGMQVFGNIALDDDTSINRHNNFRTFLQALMLLFRSATGEAWHEIMLSCLGNRACDPHANASECGSDFAYFYFVSFIFLCSFLMLNLFVAVIMDNFEYLTRDSSILGPHHLDEFIRVWAEYDPAACGRISYNDMFEMLKHMSPPLGLGKKCPARVAYKRLVRMNMPISNEDMTVHFTSTLMALIRTALEIKLAPAGTKQHQCDAELRKEISSVWANLPQKTLDLLVPPHKPDEMTVGKVYAALMIFDFYKQNKTTRDQTHQAPGGLSQMGPVSLFHPLKATLEQTQPAVLRGARVFLRQKSATSLSNGGAIQTQESGIKESLSWGTQRTQDALYEARAPLERGHSAEIPVGQSGTLAVDVQMQNMTLRGPDGEPQPGLESQGRAASMPRLAAETQPAPNASPMKRSISTLAPRPHGTQLCSTVLDRPPPSQASHHHHHRCHRRRDKKQRSLEKGPSLSVDPEGAPSTAAGPGLPHGEGSTACRRDRKQERGRSQERRQPSSSSSEKQRFYSCDRFGSREPPQLMPSLSSHPTSPTAALEPAPHPQGSGSVNGSPLMSTSGASTPGRGGRRQLPQTPLTPRPSITYKTANSSPVHFAEGQSGLPAFSPGRLSRGLSEHNALLQKEPLSQPLAPGSRIGSDPYLGQRLDSEASAHTLPEDTLTFEEAVATNSGRSSRTSYVSSLTSQSHPLRRVPNGYHCTLGLSTGVRARHSYHHPDQDHWC

Target ORF information:

RefSeq Version	XM_006497627.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus calcium channel, voltage-dependent, N type, alpha 1B subunit (Cacna1b), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006497627.3

ORF Insert Sequence:

ATGGTCCGCT TCGGGGACGA GCTAGGCGGC CGCTATGGGG GCACCGGCGG CGGGGAGCGG 
GCTCGGGGTG GCGGGGCCGG CGGGGCGGGT GGCCCGGGCC AGGGGGGTCT GCCGCCGGGC 
CAGCGGGTCC TGTACAAGCA GTCCATTGCG CAGCGCGCAC GGACTATGGC CCTGTACAAC 
CCCATCCCAG TCAAGCAGAA CTGCTTCACC GTCAACCGCT CGCTCTTCGT CTTCAGCGAG 
GACAACGTCG TCCGCAAATA CGCTAAGCGC ATCACCGAAT GGCCGCCCTT CGAATACATG 
ATCCTGGCCA CCATCATCGC CAACTGCATT GTTCTGGCCC TGGAGCAGCA CCTCCCTGAT 
GGGGACAAGA CTCCCATGTC TGAGCGACTA GATGACACGG AGCCTTACTT CATCGGGATC 
TTTTGCTTCG AGGCGGGCAT CAAGATCATA GCCCTGGGCT TTGTTTTCCA CAAGGGCTCC 
TACCTTCGGA ACGGCTGGAA TGTCATGGAC TTCGTGGTGG TCCTCACGGG GATTCTCGCC 
ACAGCTGGAA CTGACTTTGA CCTGCGCACA CTGAGGGCTG TGCGTGTGCT TAGGCCCCTG 
AAGCTGGTGT CTGGAATTCC AAGCTTGCAG GTGGTGCTTA AGTCCATCAT GAAGGCCATG 
GTCCCGCTGC TGCAGATTGG GCTGCTGCTC TTCTTTGCCA TCCTCATGTT TGCCATCATC 
GGCCTCGAAT TCTATATGGG CAAATTCCAT AAGGCCTGTT TCCCCAACAG CACAGATACA 
GAGCCTGTGG GTGACTTTCC CTGTGGCAAA GATCCCCCTG CTCGTCAGTG TGATGGTGAC 
ACCGAATGCC GGGAGTACTG GCCAGGACCC AACTTTGGCA TCACCAATTT TGACAACATC 
CTGTTTGCCA TCTTGACAGT GTTCCAGTGT ATCACCATGG AGGGCTGGAC TGACATCCTC 
TACAATACAA ATGATGCGGC TGGCAACACA TGGAACTGGT TGTACTTCAT CCCCCTCATC 
ATCATTGGCT CCTTCTTCAT GCTCAACCTG GTGCTGGGTG TGCTTTCCGG AGAGTTTGCC 
AAGGAGCGGG AGCGAGTCGA GAACCGCCGC GCCTTCCTGA AGCTCCGCAG GCAGCAGCAG 
ATTGAGCGAG AGCTGAATGG GTACTTGGAG TGGATCTTCA AGGCAGAGGA AGTCATGTTG 
GCAGAGGAGG ACAAGAATGC AGAAGAGAAA TCCCCTTTGG ATGCAGTGTT GAAGAGAGCT 
GCCACCAAGA AGAGCCGAAA TGACCTCATC CATGCAGAAG AGGGGGAGGA CCGGTTTGTA 
GACCTCTGTG CAGTTGGGTC TCCATTTGCT CGTGCCAGCC TCAAGAGTGG GAAGACGGAG 
AGCTCATCGT ACTTCCGGAG AAAGGAGAAG ATGTTCCGGT TCTTTATCCG GCGTATGGTG 
AAAGCACAGA GCTTCTACTG GGTGGTACTG TGTGTGGTGG CCCTGAACAC ACTGTGTGTG 
GCCATGGTGC ACTATAATCA GCCTCAGCGG CTTACCACTG CACTGTACTT TGCAGAGTTT 
GTTTTCCTGG GTCTCTTCCT CACAGAGATG TCCCTGAAGA TGTATGGCCT AGGGCCCAGA 
AGTTACTTCA GGTCTTCCTT CAACTGCTTT GACTTTGGGG TGATTGTGGG GAGTATCTTT 
GAAGTAGTCT GGGCTGCCAT CAAGCCAGGA ACCTCCTTTG GAATCAGTGT GCTGCGGGCT 
CTGCGACTGC TGAGGATATT CAAAGTTACC AAGTATTGGA ACTCTCTGAG GAACCTGGTG 
GTTTCCCTCC TCAATTCCAT GAAGTCCATC ATCAGCCTTC TCTTCCTGCT TTTCCTCTTC 
ATCGTGGTCT TCGCTCTGTT GGGGATGCAG CTGTTTGGGG GACAGTTCAA CTTTCAAGAT 
GAGACTCCAA CCACCAATTT TGATACCTTC CCAGCTGCCA TCCTCACTGT CTTTCAGATC 
CTGACAGGAG AGGATTGGAA TGCCGTAATG TATCATGGGA TTGAGTCGCA AGGTGGAGTC 
AGCAAAGGCA TGTTTTCTTC CTTTTACTTC ATCGTCCTGA CACTGTTTGG AAACTACACC 
CTGCTGAATG TTTTTCTGGC CATTGCTGTG GACAACCTTG CCAATGCCCA GGAGTTGACC 
AAGGATGAAG AGGAGATGGA AGAAGCAGCC AATCAGAAAC TTGCTCTTCA GAAGGCCAAA 
GAAGTAGCTG AAGTCAGCCC CATGTCTGCT GCCAATATCT CCATCGCTGC TTTTGTAAAG 
CAAACTCGAG GTACTGTATC TCGCAGCTCA TCTGTCTCCA GCGTAAACTC ACCGCAGCAG 
AACTCGGCCA AGGCGCGCTC AGTATGGGAG CAGCGGGCCA GTCAGCTAAG GCTCCAGAAT 
CTGCGTGCCA GCTGTGAGGC ATTGTACAGT GAGATGGACC CTGAGGAGCG CCTGCGTTAT 
GCCAGCACGC GCCATGTGAG GCCAGACATG AAGACACACA TGGACCGACC CCTAGTGGTG 
GAGCCTGGTC GAGATGGCTT GCGGGGACCC GTTGGGAGCA AGTCAAAGCC TGAAGGCACG 
GAGGCCACAG AAAGCGCGGA CCTACCTCGC AGGCACCACC GGCACCGTGA TAGGGACAAG 
ACCTCAGCCA CAGCACCTGC TGGAGGCGAA CAGGACAGGA CAGAAAGCAC CGAGACCGGG 
GCCCGGGAGG AACGTGCGCG CCCTCGTCGA AGTCACAGCA AGGAGACTCC AGGGGCTGAC 
ACGCAAGTGC GCTGTGAGCG CAGTAGACGT CACCACCGGC GCGGCTCCCC GGAGGAGGCC 
ACTGAACGGG AGCCTCGGCG CCACCGTGCC CACCGGCATG CACAGGACTC AAGCAAGGAG 
GGCACGGCGC CGGTGCTTGT ACCCAAGGGT GAGCGACGAG CAAGACACCG AGGCCCACGC 
ACGGGTCCAC GTGAGGCAGA GAACAACGAG GAGCCCACAC GCAGGCACCG TGCAAGGCAT 
AAGGTGCCAC CCACACTGCA GCCCCCAGAG AGGGAGGCTG CAGAGAAGGA GAGCAACGCG 
GTGGAAGGGG ATAAGGAAAC CCGAAATCAC CAGCCCAAGG AACCTCACTG TGACCTGGAG 
GCCATTGCAG TTACAGGTGT GGGCCCTCTG CACATGCTGC CCAGCACCTG TCTCCAGAAA 
GTGGACGAGC AACCAGAGGA TGCAGACAAC CAGCGTAATG TCACCCGGAT GGGCAGTCAG 
CCCTCAGATC CCAGCACCAC TGTGCATGTC CCAGTGACAC TGACAGGCCC TCCTGGGGAG 
ACCCCTGTAG TTCCCAGTGG TAACATGAAC CTGGAAGGCC AAGCAGAGGG CAAGAAGGAG 
GCAGAGGCGG ATGATGTGCT GAGAAGAGGC CCCAGGCCCA TCGTTCCCTA CAGCTCCATG 
TTTTGTCTCA GCCCCACCAA CCTGCTTCGT CGCTTCTGCC ATTACATTGT GACCATGCGG 
TACTTTGAGA TGGTAATTCT TGTGGTCATT GCCTTGAGCA GCATTGCCCT GGCTGCAGAG 
GATCCTGTGC GGACAGATTC ATTCAGGAAC AACGCTTTAA AGTACATGGA TTACATCTTT 
ACAGGAGTCT TCACCTTTGA AATGGTCATA AAGATGATAG ACTTGGGCTT GCTGCTGCAC 
CCTGGTGCCT ACTTCCGGGA CCTGTGGAAC ATTCTGGACT TCATTGTTGT CAGTGGAGCC 
CTGGTGGCAT TTGCGTTCTC GAGCTTCATG GGAGGATCCA AAGGGAAAGA CATCAATACC 
ATCAAGTCTC TGAGAGTCCT GCGTGTCCTG AGGCCCCTCA AGACCATCAA GCGGCTGCCT 
AAGCTCAAGG CTGTCTTTGA CTGTGTGGTG AACTCCCTGA AGAACGTCTT GAACATCCTG 
ATTGTCTACA TGCTCTTCAT GTTCATATTT GCCGTCATTG CCGTCCAGCT CTTCAAAGGG 
AAGTTCTTTT ACTGTACTGA TGAATCCAAG GAGCTGGAGA GGGACTGCCG GGGTCAGTAT 
TTGGATTATG AGAAGGAAGA AGTAGAAGCC CAGCCAAGGC AGTGGAAGAA ATATGACTTC 
CACTATGACA ATGTTCTCTG GGCCTTGTTG ACGCTGTTCA CAGTGTCCAC GGGAGAGGGG 
TGGCCCATGG TGCTGAAACA CTCTGTGGAT GCCACCTATG AGGAACAGGG GCCCAGTCCC 
GGGTTCCGGA TGGAGCTCTC CATCTTCTAC GTGGTCTACT TTGTGGTCTT CCCTTTTTTC 
TTTGTCAACA TCTTTGTGGC CTTGATCATT ATCACCTTCC AGGAACAGGG AGATAAGGTG 
ATGTCTGAAT GCAGCTTAGA AAAGAATGAG AGGGCTTGCA TTGATTTTGC CATCAGTGCC 
AAGCCCCTGA CACGGTACAT GCCTCAAAAC AAACAGTCGT TCCAGTATAA GACATGGACA 
TTCGTGGTCT CTCCACCCTT TGAGTACTTC ATCATGGCTA TGATAGCCCT CAACACAGTG 
GTGCTGATGA TGAAGTTCTA TGATGCACCT TATGAGTACG AGCTGATGCT GAAATGCCTG 
AACATTGTCT TCACATCCAT GTTCTCGATG GAGTGCATAC TGAAGATCAT CGCCTTTGGG 
GTATTGAACT ACTTCAGAGA TGCCTGGAAC GTCTTTGACT TTGTCACGGT TTTGGGAAGT 
ATTACTGATA TTTTAGTAAC AGAGATAGCG GAAACGAACA ACTTCATCAA CCTAAGCTTC 
CTTCGCCTCT TCCGGGCGGC ACGGCTGATC AAGCTGCTTC GCCAGGGCTA CACCATCCGC 
ATCCTATTGT GGACCTTCGT CCAGTCCTTT AAGGCGCTGC CCTACGTGTG CCTCCTCATT 
GCCATGCTGT TCTTCATCTA CGCCATCATC GGCATGCAGG TTTTTGGAAA CATTGCCCTT 
GATGATGACA CCAGTATCAA CCGACACAAC AACTTCCGGA CATTTCTGCA AGCCTTAATG 
CTATTGTTCA GGAGTGCCAC TGGGGAGGCC TGGCATGAGA TCATGCTGTC TTGTCTGGGC 
AACCGGGCCT GTGACCCACA TGCCAACGCC AGTGAGTGCG GGAGCGACTT TGCCTATTTT 
TATTTTGTCT CCTTCATCTT CCTCTGTTCC TTTCTGATGT TGAACCTCTT TGTTGCTGTA 
ATCATGGACA ATTTTGAGTA CCTCACTCGG GACTCTTCCA TCCTAGGGCC TCACCACTTA 
GACGAATTCA TTCGAGTCTG GGCTGAATAC GACCCAGCTG CGTGTGGGCG CATCAGTTAC 
AATGACATGT TTGAGATGCT GAAACACATG TCCCCACCTC TGGGGTTGGG GAAGAAATGC 
CCGGCTCGAG TTGCATACAA GCGCCTGGTT CGCATGAACA TGCCCATATC CAATGAGGAC 
ATGACGGTGC ACTTTACGTC CACACTGATG GCCCTCATCC GGACAGCACT GGAGATCAAG 
CTTGCCCCAG CGGGGACGAA GCAGCACCAG TGTGATGCTG AGCTGAGAAA AGAGATCTCT 
TCTGTGTGGG CTAATCTGCC CCAGAAGACT CTGGACTTAC TGGTACCACC CCACAAACCT 
GACGAGATGA CAGTGGGGAA GGTCTATGCT GCTCTCATGA TATTTGACTT CTACAAACAG 
AACAAAACCA CCAGAGATCA GACTCACCAA GCTCCCGGAG GCCTGTCCCA GATGGGTCCC 
GTTTCCCTGT TCCACCCTCT GAAGGCCACC CTGGAACAGA CACAGCCCGC TGTGCTTCGA 
GGAGCTCGGG TTTTCCTTCG GCAAAAGAGT GCAACTTCCC TCAGCAATGG GGGTGCCATA 
CAAACCCAGG AAAGTGGCAT CAAGGAGTCG CTGTCCTGGG GCACGCAGAG GACCCAAGAT 
GCACTTTATG AGGCCAGAGC ACCTCTAGAA CGTGGCCATT CTGCAGAGAT CCCTGTGGGG 
CAGTCAGGAA CACTGGCTGT GGATGTCCAG ATGCAGAACA TGACACTGAG AGGACCAGAT 
GGGGAGCCCC AGCCTGGGCT GGAAAGCCAA GGCAGAGCTG CCTCTATGCC ACGCCTAGCG 
GCAGAAACAC AGCCGGCCCC TAATGCCAGC CCCATGAAGC GCTCCATCTC CACACTGGCT 
CCACGCCCAC ATGGGACTCA GCTTTGCAGC ACAGTTCTGG ACCGGCCTCC TCCTAGCCAG 
GCATCACATC ACCACCACCA CCGCTGCCAC CGGCGCAGAG ACAAGAAGCA AAGGTCCCTG 
GAAAAGGGGC CCAGCCTGTC TGTTGACCCA GAAGGTGCAC CAAGCACTGC TGCAGGACCT 
GGTCTGCCCC ATGGAGAAGG ATCCACCGCC TGCCGGCGGG ACCGTAAACA GGAGCGAGGC 
CGGTCCCAGG AGCGGAGGCA GCCCTCATCT TCCTCTTCAG AGAAGCAGCG CTTCTATTCC 
TGTGACCGCT TTGGGAGCCG GGAGCCCCCG CAACTGATGC CCTCACTCAG TAGCCACCCC 
ACATCGCCAA CAGCGGCGCT AGAGCCAGCA CCCCACCCAC AGGGCAGTGG TTCCGTTAAT 
GGGAGCCCCT TGATGTCAAC ATCCGGTGCT AGCACCCCGG GCCGAGGTGG GCGGAGGCAG 
CTCCCCCAGA CTCCTCTGAC CCCACGCCCC AGCATCACCT ACAAGACCGC CAATTCCTCG 
CCTGTCCACT TTGCTGAGGG TCAGAGCGGC CTCCCAGCCT TCTCCCCTGG CCGTCTCAGC 
CGCGGCCTTT CTGAACACAA TGCCCTGCTC CAGAAAGAGC CCCTGAGCCA GCCTCTAGCT 
CCTGGCTCCC GAATTGGCTC TGACCCTTAC CTAGGGCAGC GTCTGGACAG TGAGGCCTCC 
GCCCACACCC TGCCTGAGGA TACACTCACC TTTGAAGAGG CAGTGGCCAC CAACTCTGGC 
CGCTCCTCCA GGACTTCCTA TGTGTCCTCC CTCACTTCCC AATCCCACCC TCTCCGCCGT 
GTACCCAATG GCTATCACTG CACTTTGGGA CTCAGCACTG GCGTCCGGGC GCGGCACAGC 
TACCACCACC CCGATCAGGA CCACTGGTGC TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cacna1b ( XM_006497628.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006497691.1 Mus musculus calcium channel, voltage-dependent, N type, alpha 1B subunit (Cacna1b), transcript variant X5, mRNA.

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CloneID	OMu29259
Clone ID Related Accession (Same CDS sequence)	XM_006497628.3
Accession Version	XM_006497628.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7044bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	voltage-dependent N-type calcium channel subunit alpha-1B isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006497628.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTCCGCT TCGGGGACGA GCTAGGCGGC CGCTATGGGG GCACCGGCGG CGGGGAGCGG 
GCTCGGGGTG GCGGGGCCGG CGGGGCGGGT GGCCCGGGCC AGGGGGGTCT GCCGCCGGGC 
CAGCGGGTCC TGTACAAGCA GTCCATTGCG CAGCGCGCAC GGACTATGGC CCTGTACAAC 
CCCATCCCAG TCAAGCAGAA CTGCTTCACC GTCAACCGCT CGCTCTTCGT CTTCAGCGAG 
GACAACGTCG TCCGCAAATA CGCTAAGCGC ATCACCGAAT GGCCGCCCTT CGAATACATG 
ATCCTGGCCA CCATCATCGC CAACTGCATT GTTCTGGCCC TGGAGCAGCA CCTCCCTGAT 
GGGGACAAGA CTCCCATGTC TGAGCGACTA GATGACACGG AGCCTTACTT CATCGGGATC 
TTTTGCTTCG AGGCGGGCAT CAAGATCATA GCCCTGGGCT TTGTTTTCCA CAAGGGCTCC 
TACCTTCGGA ACGGCTGGAA TGTCATGGAC TTCGTGGTGG TCCTCACGGG GATTCTCGCC 
ACAGCTGGAA CTGACTTTGA CCTGCGCACA CTGAGGGCTG TGCGTGTGCT TAGGCCCCTG 
AAGCTGGTGT CTGGAATTCC AAGCTTGCAG GTGGTGCTTA AGTCCATCAT GAAGGCCATG 
GTCCCGCTGC TGCAGATTGG GCTGCTGCTC TTCTTTGCCA TCCTCATGTT TGCCATCATC 
GGCCTCGAAT TCTATATGGG CAAATTCCAT AAGGCCTGTT TCCCCAACAG CACAGATACA 
GAGCCTGTGG GTGACTTTCC CTGTGGCAAA GATCCCCCTG CTCGTCAGTG TGATGGTGAC 
ACCGAATGCC GGGAGTACTG GCCAGGACCC AACTTTGGCA TCACCAATTT TGACAACATC 
CTGTTTGCCA TCTTGACAGT GTTCCAGTGT ATCACCATGG AGGGCTGGAC TGACATCCTC 
TACAATACAA ATGATGCGGC TGGCAACACA TGGAACTGGT TGTACTTCAT CCCCCTCATC 
ATCATTGGCT CCTTCTTCAT GCTCAACCTG GTGCTGGGTG TGCTTTCCGG AGAGTTTGCC 
AAGGAGCGGG AGCGAGTCGA GAACCGCCGC GCCTTCCTGA AGCTCCGCAG GCAGCAGCAG 
ATTGAGCGAG AGCTGAATGG GTACTTGGAG TGGATCTTCA AGGCAGAGGA AGTCATGTTG 
GCAGAGGAGG ACAAGAATGC AGAAGAGAAA TCCCCTTTGG ATGCAGTGTT GAAGAGAGCT 
GCCACCAAGA AGAGCCGAAA TGACCTCATC CATGCAGAAG AGGGGGAGGA CCGGTTTGTA 
GACCTCTGTG CAGTTGGGTC TCCATTTGCT CGTGCCAGCC TCAAGAGTGG GAAGACGGAG 
AGCTCATCGT ACTTCCGGAG AAAGGAGAAG ATGTTCCGGT TCTTTATCCG GCGTATGGTG 
AAAGCACAGA GCTTCTACTG GGTGGTACTG TGTGTGGTGG CCCTGAACAC ACTGTGTGTG 
GCCATGGTGC ACTATAATCA GCCTCAGCGG CTTACCACTG CACTGTACTT TGCAGAGTTT 
GTTTTCCTGG GTCTCTTCCT CACAGAGATG TCCCTGAAGA TGTATGGCCT AGGGCCCAGA 
AGTTACTTCA GGTCTTCCTT CAACTGCTTT GACTTTGGGG TGATTGTGGG GAGTATCTTT 
GAAGTAGTCT GGGCTGCCAT CAAGCCAGGA ACCTCCTTTG GAATCAGTGT GCTGCGGGCT 
CTGCGACTGC TGAGGATATT CAAAGTTACC AAGTATTGGA ACTCTCTGAG GAACCTGGTG 
GTTTCCCTCC TCAATTCCAT GAAGTCCATC ATCAGCCTTC TCTTCCTGCT TTTCCTCTTC 
ATCGTGGTCT TCGCTCTGTT GGGGATGCAG CTGTTTGGGG GACAGTTCAA CTTTCAAGAT 
GAGACTCCAA CCACCAATTT TGATACCTTC CCAGCTGCCA TCCTCACTGT CTTTCAGATC 
CTGACAGGAG AGGATTGGAA TGCCGTAATG TATCATGGGA TTGAGTCGCA AGGTGGAGTC 
AGCAAAGGCA TGTTTTCTTC CTTTTACTTC ATCGTCCTGA CACTGTTTGG AAACTACACC 
CTGCTGAATG TTTTTCTGGC CATTGCTGTG GACAACCTTG CCAATGCCCA GGAGTTGACC 
AAGGATGAAG AGGAGATGGA AGAAGCAGCC AATCAGAAAC TTGCTCTTCA GAAGGCCAAA 
GAAGTAGCTG AAGTCAGCCC CATGTCTGCT GCCAATATCT CCATCGCTGC TTTTGTAAAG 
CAAACTCGAG GTACTGTATC TCGCAGCTCA TCTGTCTCCA GCGTAAACTC ACCCAGGCAG 
CAGAACTCGG CCAAGGCGCG CTCAGTATGG GAGCAGCGGG CCAGTCAGCT AAGGCTCCAG 
AATCTGCGTG CCAGCTGTGA GGCATTGTAC AGTGAGATGG ACCCTGAGGA GCGCCTGCGT 
TATGCCAGCA CGCGCCATGT GAGGCCAGAC ATGAAGACAC ACATGGACCG ACCCCTAGTG 
GTGGAGCCTG GTCGAGATGG CTTGCGGGGA CCCGTTGGGA GCAAGTCAAA GCCTGAAGGC 
ACGGAGGCCA CAGAAAGCGC GGACCTACCT CGCAGGCACC ACCGGCACCG TGATAGGGAC 
AAGACCTCAG CCACAGCACC TGCTGGAGGC GAACAGGACA GGACAGAAAG CACCGAGACC 
GGGGCCCGGG AGGAACGTGC GCGCCCTCGT CGAAGTCACA GCAAGGAGAC TCCAGGGGCT 
GACACGCAAG TGCGCTGTGA GCGCAGTAGA CGTCACCACC GGCGCGGCTC CCCGGAGGAG 
GCCACTGAAC GGGAGCCTCG GCGCCACCGT GCCCACCGGC ATGCACAGGA CTCAAGCAAG 
GAGGGCACGG CGCCGGTGCT TGTACCCAAG GGTGAGCGAC GAGCAAGACA CCGAGGCCCA 
CGCACGGGTC CACGTGAGGC AGAGAACAAC GAGGAGCCCA CACGCAGGCA CCGTGCAAGG 
CATAAGGTGC CACCCACACT GCAGCCCCCA GAGAGGGAGG CTGCAGAGAA GGAGAGCAAC 
GCGGTGGAAG GGGATAAGGA AACCCGAAAT CACCAGCCCA AGGAACCTCA CTGTGACCTG 
GAGGCCATTG CAGTTACAGG TGTGGGCCCT CTGCACATGC TGCCCAGCAC CTGTCTCCAG 
AAAGTGGACG AGCAACCAGA GGATGCAGAC AACCAGCGTA ATGTCACCCG GATGGGCAGT 
CAGCCCTCAG ATCCCAGCAC CACTGTGCAT GTCCCAGTGA CACTGACAGG CCCTCCTGGG 
GAGACCCCTG TAGTTCCCAG TGGTAACATG AACCTGGAAG GCCAAGCAGA GGGCAAGAAG 
GAGGCAGAGG CGGATGATGT GCTGAGAAGA GGCCCCAGGC CCATCGTTCC CTACAGCTCC 
ATGTTTTGTC TCAGCCCCAC CAACCTGCTT CGTCGCTTCT GCCATTACAT TGTGACCATG 
CGGTACTTTG AGATGGTAAT TCTTGTGGTC ATTGCCTTGA GCAGCATTGC CCTGGCTGCA 
GAGGATCCTG TGCGGACAGA TTCATTCAGG AACAACGCTT TAAAGTACAT GGATTACATC 
TTTACAGGAG TCTTCACCTT TGAAATGGTC ATAAAGATGA TAGACTTGGG CTTGCTGCTG 
CACCCTGGTG CCTACTTCCG GGACCTGTGG AACATTCTGG ACTTCATTGT TGTCAGTGGA 
GCCCTGGTGG CATTTGCGTT CTCAGGATCC AAAGGGAAAG ACATCAATAC CATCAAGTCT 
CTGAGAGTCC TGCGTGTCCT GAGGCCCCTC AAGACCATCA AGCGGCTGCC TAAGCTCAAG 
GCTGTCTTTG ACTGTGTGGT GAACTCCCTG AAGAACGTCT TGAACATCCT GATTGTCTAC 
ATGCTCTTCA TGTTCATATT TGCCGTCATT GCCGTCCAGC TCTTCAAAGG GAAGTTCTTT 
TACTGTACTG ATGAATCCAA GGAGCTGGAG AGGGACTGCC GGGGTCAGTA TTTGGATTAT 
GAGAAGGAAG AAGTAGAAGC CCAGCCAAGG CAGTGGAAGA AATATGACTT CCACTATGAC 
AATGTTCTCT GGGCCTTGTT GACGCTGTTC ACAGTGTCCA CGGGAGAGGG GTGGCCCATG 
GTGCTGAAAC ACTCTGTGGA TGCCACCTAT GAGGAACAGG GGCCCAGTCC CGGGTTCCGG 
ATGGAGCTCT CCATCTTCTA CGTGGTCTAC TTTGTGGTCT TCCCTTTTTT CTTTGTCAAC 
ATCTTTGTGG CCTTGATCAT TATCACCTTC CAGGAACAGG GAGATAAGGT GATGTCTGAA 
TGCAGCTTAG AAAAGAATGA GAGGGCTTGC ATTGATTTTG CCATCAGTGC CAAGCCCCTG 
ACACGGTACA TGCCTCAAAA CAAACAGTCG TTCCAGTATA AGACATGGAC ATTCGTGGTC 
TCTCCACCCT TTGAGTACTT CATCATGGCT ATGATAGCCC TCAACACAGT GGTGCTGATG 
ATGAAGTTCT ATGATGCACC TTATGAGTAC GAGCTGATGC TGAAATGCCT GAACATTGTC 
TTCACATCCA TGTTCTCGAT GGAGTGCATA CTGAAGATCA TCGCCTTTGG GGTATTGAAC 
TACTTCAGAG ATGCCTGGAA CGTCTTTGAC TTTGTCACGG TTTTGGGAAG TATTACTGAT 
ATTTTAGTAA CAGAGATAGC GGAAACGAAC AACTTCATCA ACCTAAGCTT CCTTCGCCTC 
TTCCGGGCGG CACGGCTGAT CAAGCTGCTT CGCCAGGGCT ACACCATCCG CATCCTATTG 
TGGACCTTCG TCCAGTCCTT TAAGGCGCTG CCCTACGTGT GCCTCCTCAT TGCCATGCTG 
TTCTTCATCT ACGCCATCAT CGGCATGCAG GTTTTTGGAA ACATTGCCCT TGATGATGAC 
ACCAGTATCA ACCGACACAA CAACTTCCGG ACATTTCTGC AAGCCTTAAT GCTATTGTTC 
AGGAGTGCCA CTGGGGAGGC CTGGCATGAG ATCATGCTGT CTTGTCTGGG CAACCGGGCC 
TGTGACCCAC ATGCCAACGC CAGTGAGTGC GGGAGCGACT TTGCCTATTT TTATTTTGTC 
TCCTTCATCT TCCTCTGTTC CTTTCTGATG TTGAACCTCT TTGTTGCTGT AATCATGGAC 
AATTTTGAGT ACCTCACTCG GGACTCTTCC ATCCTAGGGC CTCACCACTT AGACGAATTC 
ATTCGAGTCT GGGCTGAATA CGACCCAGCT GCGTGTGGGC GCATCAGTTA CAATGACATG 
TTTGAGATGC TGAAACACAT GTCCCCACCT CTGGGGTTGG GGAAGAAATG CCCGGCTCGA 
GTTGCATACA AGCGCCTGGT TCGCATGAAC ATGCCCATAT CCAATGAGGA CATGACGGTG 
CACTTTACGT CCACACTGAT GGCCCTCATC CGGACAGCAC TGGAGATCAA GCTTGCCCCA 
GCGGGGACGA AGCAGCACCA GTGTGATGCT GAGCTGAGAA AAGAGATCTC TTCTGTGTGG 
GCTAATCTGC CCCAGAAGAC TCTGGACTTA CTGGTACCAC CCCACAAACC TGACGAGATG 
ACAGTGGGGA AGGTCTATGC TGCTCTCATG ATATTTGACT TCTACAAACA GAACAAAACC 
ACCAGAGATC AGACTCACCA AGCTCCCGGA GGCCTGTCCC AGATGGGTCC CGTTTCCCTG 
TTCCACCCTC TGAAGGCCAC CCTGGAACAG ACACAGCCCG CTGTGCTTCG AGGAGCTCGG 
GTTTTCCTTC GGCAAAAGAG TGCAACTTCC CTCAGCAATG GGGGTGCCAT ACAAACCCAG 
GAAAGTGGCA TCAAGGAGTC GCTGTCCTGG GGCACGCAGA GGACCCAAGA TGCACTTTAT 
GAGGCCAGAG CACCTCTAGA ACGTGGCCAT TCTGCAGAGA TCCCTGTGGG GCAGTCAGGA 
ACACTGGCTG TGGATGTCCA GATGCAGAAC ATGACACTGA GAGGACCAGA TGGGGAGCCC 
CAGCCTGGGC TGGAAAGCCA AGGCAGAGCT GCCTCTATGC CACGCCTAGC GGCAGAAACA 
CAGCCGGCCC CTAATGCCAG CCCCATGAAG CGCTCCATCT CCACACTGGC TCCACGCCCA 
CATGGGACTC AGCTTTGCAG CACAGTTCTG GACCGGCCTC CTCCTAGCCA GGCATCACAT 
CACCACCACC ACCGCTGCCA CCGGCGCAGA GACAAGAAGC AAAGGTCCCT GGAAAAGGGG 
CCCAGCCTGT CTGTTGACCC AGAAGGTGCA CCAAGCACTG CTGCAGGACC TGGTCTGCCC 
CATGGAGAAG GATCCACCGC CTGCCGGCGG GACCGTAAAC AGGAGCGAGG CCGGTCCCAG 
GAGCGGAGGC AGCCCTCATC TTCCTCTTCA GAGAAGCAGC GCTTCTATTC CTGTGACCGC 
TTTGGGAGCC GGGAGCCCCC GCAACTGATG CCCTCACTCA GTAGCCACCC CACATCGCCA 
ACAGCGGCGC TAGAGCCAGC ACCCCACCCA CAGGGCAGTG GTTCCGTTAA TGGGAGCCCC 
TTGATGTCAA CATCCGGTGC TAGCACCCCG GGCCGAGGTG GGCGGAGGCA GCTCCCCCAG 
ACTCCTCTGA CCCCACGCCC CAGCATCACC TACAAGACCG CCAATTCCTC GCCTGTCCAC 
TTTGCTGAGG GTCAGAGCGG CCTCCCAGCC TTCTCCCCTG GCCGTCTCAG CCGCGGCCTT 
TCTGAACACA ATGCCCTGCT CCAGAAAGAG CCCCTGAGCC AGCCTCTAGC TCCTGGCTCC 
CGAATTGGCT CTGACCCTTA CCTAGGGCAG CGTCTGGACA GTGAGGCCTC CGCCCACACC 
CTGCCTGAGG ATACACTCAC CTTTGAAGAG GCAGTGGCCA CCAACTCTGG CCGCTCCTCC 
AGGACTTCCT ATGTGTCCTC CCTCACTTCC CAATCCCACC CTCTCCGCCG TGTACCCAAT 
GGCTATCACT GCACTTTGGG ACTCAGCACT GGCGTCCGGG CGCGGCACAG CTACCACCAC 
CCCGATCAGG ACCACTGGTG CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006497691.1
CDS	146..7189
Translation	MVRFGDELGGRYGGTGGGERARGGGAGGAGGPGQGGLPPGQRVLYKQSIAQRARTMALYNPIPVKQNCFTVNRSLFVFSEDNVVRKYAKRITEWPPFEYMILATIIANCIVLALEQHLPDGDKTPMSERLDDTEPYFIGIFCFEAGIKIIALGFVFHKGSYLRNGWNVMDFVVVLTGILATAGTDFDLRTLRAVRVLRPLKLVSGIPSLQVVLKSIMKAMVPLLQIGLLLFFAILMFAIIGLEFYMGKFHKACFPNSTDTEPVGDFPCGKDPPARQCDGDTECREYWPGPNFGITNFDNILFAILTVFQCITMEGWTDILYNTNDAAGNTWNWLYFIPLIIIGSFFMLNLVLGVLSGEFAKERERVENRRAFLKLRRQQQIERELNGYLEWIFKAEEVMLAEEDKNAEEKSPLDAVLKRAATKKSRNDLIHAEEGEDRFVDLCAVGSPFARASLKSGKTESSSYFRRKEKMFRFFIRRMVKAQSFYWVVLCVVALNTLCVAMVHYNQPQRLTTALYFAEFVFLGLFLTEMSLKMYGLGPRSYFRSSFNCFDFGVIVGSIFEVVWAAIKPGTSFGISVLRALRLLRIFKVTKYWNSLRNLVVSLLNSMKSIISLLFLLFLFIVVFALLGMQLFGGQFNFQDETPTTNFDTFPAAILTVFQILTGEDWNAVMYHGIESQGGVSKGMFSSFYFIVLTLFGNYTLLNVFLAIAVDNLANAQELTKDEEEMEEAANQKLALQKAKEVAEVSPMSAANISIAAFVKQTRGTVSRSSSVSSVNSPRQQNSAKARSVWEQRASQLRLQNLRASCEALYSEMDPEERLRYASTRHVRPDMKTHMDRPLVVEPGRDGLRGPVGSKSKPEGTEATESADLPRRHHRHRDRDKTSATAPAGGEQDRTESTETGAREERARPRRSHSKETPGADTQVRCERSRRHHRRGSPEEATEREPRRHRAHRHAQDSSKEGTAPVLVPKGERRARHRGPRTGPREAENNEEPTRRHRARHKVPPTLQPPEREAAEKESNAVEGDKETRNHQPKEPHCDLEAIAVTGVGPLHMLPSTCLQKVDEQPEDADNQRNVTRMGSQPSDPSTTVHVPVTLTGPPGETPVVPSGNMNLEGQAEGKKEAEADDVLRRGPRPIVPYSSMFCLSPTNLLRRFCHYIVTMRYFEMVILVVIALSSIALAAEDPVRTDSFRNNALKYMDYIFTGVFTFEMVIKMIDLGLLLHPGAYFRDLWNILDFIVVSGALVAFAFSGSKGKDINTIKSLRVLRVLRPLKTIKRLPKLKAVFDCVVNSLKNVLNILIVYMLFMFIFAVIAVQLFKGKFFYCTDESKELERDCRGQYLDYEKEEVEAQPRQWKKYDFHYDNVLWALLTLFTVSTGEGWPMVLKHSVDATYEEQGPSPGFRMELSIFYVVYFVVFPFFFVNIFVALIIITFQEQGDKVMSECSLEKNERACIDFAISAKPLTRYMPQNKQSFQYKTWTFVVSPPFEYFIMAMIALNTVVLMMKFYDAPYEYELMLKCLNIVFTSMFSMECILKIIAFGVLNYFRDAWNVFDFVTVLGSITDILVTEIAETNNFINLSFLRLFRAARLIKLLRQGYTIRILLWTFVQSFKALPYVCLLIAMLFFIYAIIGMQVFGNIALDDDTSINRHNNFRTFLQALMLLFRSATGEAWHEIMLSCLGNRACDPHANASECGSDFAYFYFVSFIFLCSFLMLNLFVAVIMDNFEYLTRDSSILGPHHLDEFIRVWAEYDPAACGRISYNDMFEMLKHMSPPLGLGKKCPARVAYKRLVRMNMPISNEDMTVHFTSTLMALIRTALEIKLAPAGTKQHQCDAELRKEISSVWANLPQKTLDLLVPPHKPDEMTVGKVYAALMIFDFYKQNKTTRDQTHQAPGGLSQMGPVSLFHPLKATLEQTQPAVLRGARVFLRQKSATSLSNGGAIQTQESGIKESLSWGTQRTQDALYEARAPLERGHSAEIPVGQSGTLAVDVQMQNMTLRGPDGEPQPGLESQGRAASMPRLAAETQPAPNASPMKRSISTLAPRPHGTQLCSTVLDRPPPSQASHHHHHRCHRRRDKKQRSLEKGPSLSVDPEGAPSTAAGPGLPHGEGSTACRRDRKQERGRSQERRQPSSSSSEKQRFYSCDRFGSREPPQLMPSLSSHPTSPTAALEPAPHPQGSGSVNGSPLMSTSGASTPGRGGRRQLPQTPLTPRPSITYKTANSSPVHFAEGQSGLPAFSPGRLSRGLSEHNALLQKEPLSQPLAPGSRIGSDPYLGQRLDSEASAHTLPEDTLTFEEAVATNSGRSSRTSYVSSLTSQSHPLRRVPNGYHCTLGLSTGVRARHSYHHPDQDHWC

Target ORF information:

RefSeq Version	XM_006497628.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus calcium channel, voltage-dependent, N type, alpha 1B subunit (Cacna1b), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006497628.3

ORF Insert Sequence:

ATGGTCCGCT TCGGGGACGA GCTAGGCGGC CGCTATGGGG GCACCGGCGG CGGGGAGCGG 
GCTCGGGGTG GCGGGGCCGG CGGGGCGGGT GGCCCGGGCC AGGGGGGTCT GCCGCCGGGC 
CAGCGGGTCC TGTACAAGCA GTCCATTGCG CAGCGCGCAC GGACTATGGC CCTGTACAAC 
CCCATCCCAG TCAAGCAGAA CTGCTTCACC GTCAACCGCT CGCTCTTCGT CTTCAGCGAG 
GACAACGTCG TCCGCAAATA CGCTAAGCGC ATCACCGAAT GGCCGCCCTT CGAATACATG 
ATCCTGGCCA CCATCATCGC CAACTGCATT GTTCTGGCCC TGGAGCAGCA CCTCCCTGAT 
GGGGACAAGA CTCCCATGTC TGAGCGACTA GATGACACGG AGCCTTACTT CATCGGGATC 
TTTTGCTTCG AGGCGGGCAT CAAGATCATA GCCCTGGGCT TTGTTTTCCA CAAGGGCTCC 
TACCTTCGGA ACGGCTGGAA TGTCATGGAC TTCGTGGTGG TCCTCACGGG GATTCTCGCC 
ACAGCTGGAA CTGACTTTGA CCTGCGCACA CTGAGGGCTG TGCGTGTGCT TAGGCCCCTG 
AAGCTGGTGT CTGGAATTCC AAGCTTGCAG GTGGTGCTTA AGTCCATCAT GAAGGCCATG 
GTCCCGCTGC TGCAGATTGG GCTGCTGCTC TTCTTTGCCA TCCTCATGTT TGCCATCATC 
GGCCTCGAAT TCTATATGGG CAAATTCCAT AAGGCCTGTT TCCCCAACAG CACAGATACA 
GAGCCTGTGG GTGACTTTCC CTGTGGCAAA GATCCCCCTG CTCGTCAGTG TGATGGTGAC 
ACCGAATGCC GGGAGTACTG GCCAGGACCC AACTTTGGCA TCACCAATTT TGACAACATC 
CTGTTTGCCA TCTTGACAGT GTTCCAGTGT ATCACCATGG AGGGCTGGAC TGACATCCTC 
TACAATACAA ATGATGCGGC TGGCAACACA TGGAACTGGT TGTACTTCAT CCCCCTCATC 
ATCATTGGCT CCTTCTTCAT GCTCAACCTG GTGCTGGGTG TGCTTTCCGG AGAGTTTGCC 
AAGGAGCGGG AGCGAGTCGA GAACCGCCGC GCCTTCCTGA AGCTCCGCAG GCAGCAGCAG 
ATTGAGCGAG AGCTGAATGG GTACTTGGAG TGGATCTTCA AGGCAGAGGA AGTCATGTTG 
GCAGAGGAGG ACAAGAATGC AGAAGAGAAA TCCCCTTTGG ATGCAGTGTT GAAGAGAGCT 
GCCACCAAGA AGAGCCGAAA TGACCTCATC CATGCAGAAG AGGGGGAGGA CCGGTTTGTA 
GACCTCTGTG CAGTTGGGTC TCCATTTGCT CGTGCCAGCC TCAAGAGTGG GAAGACGGAG 
AGCTCATCGT ACTTCCGGAG AAAGGAGAAG ATGTTCCGGT TCTTTATCCG GCGTATGGTG 
AAAGCACAGA GCTTCTACTG GGTGGTACTG TGTGTGGTGG CCCTGAACAC ACTGTGTGTG 
GCCATGGTGC ACTATAATCA GCCTCAGCGG CTTACCACTG CACTGTACTT TGCAGAGTTT 
GTTTTCCTGG GTCTCTTCCT CACAGAGATG TCCCTGAAGA TGTATGGCCT AGGGCCCAGA 
AGTTACTTCA GGTCTTCCTT CAACTGCTTT GACTTTGGGG TGATTGTGGG GAGTATCTTT 
GAAGTAGTCT GGGCTGCCAT CAAGCCAGGA ACCTCCTTTG GAATCAGTGT GCTGCGGGCT 
CTGCGACTGC TGAGGATATT CAAAGTTACC AAGTATTGGA ACTCTCTGAG GAACCTGGTG 
GTTTCCCTCC TCAATTCCAT GAAGTCCATC ATCAGCCTTC TCTTCCTGCT TTTCCTCTTC 
ATCGTGGTCT TCGCTCTGTT GGGGATGCAG CTGTTTGGGG GACAGTTCAA CTTTCAAGAT 
GAGACTCCAA CCACCAATTT TGATACCTTC CCAGCTGCCA TCCTCACTGT CTTTCAGATC 
CTGACAGGAG AGGATTGGAA TGCCGTAATG TATCATGGGA TTGAGTCGCA AGGTGGAGTC 
AGCAAAGGCA TGTTTTCTTC CTTTTACTTC ATCGTCCTGA CACTGTTTGG AAACTACACC 
CTGCTGAATG TTTTTCTGGC CATTGCTGTG GACAACCTTG CCAATGCCCA GGAGTTGACC 
AAGGATGAAG AGGAGATGGA AGAAGCAGCC AATCAGAAAC TTGCTCTTCA GAAGGCCAAA 
GAAGTAGCTG AAGTCAGCCC CATGTCTGCT GCCAATATCT CCATCGCTGC TTTTGTAAAG 
CAAACTCGAG GTACTGTATC TCGCAGCTCA TCTGTCTCCA GCGTAAACTC ACCCAGGCAG 
CAGAACTCGG CCAAGGCGCG CTCAGTATGG GAGCAGCGGG CCAGTCAGCT AAGGCTCCAG 
AATCTGCGTG CCAGCTGTGA GGCATTGTAC AGTGAGATGG ACCCTGAGGA GCGCCTGCGT 
TATGCCAGCA CGCGCCATGT GAGGCCAGAC ATGAAGACAC ACATGGACCG ACCCCTAGTG 
GTGGAGCCTG GTCGAGATGG CTTGCGGGGA CCCGTTGGGA GCAAGTCAAA GCCTGAAGGC 
ACGGAGGCCA CAGAAAGCGC GGACCTACCT CGCAGGCACC ACCGGCACCG TGATAGGGAC 
AAGACCTCAG CCACAGCACC TGCTGGAGGC GAACAGGACA GGACAGAAAG CACCGAGACC 
GGGGCCCGGG AGGAACGTGC GCGCCCTCGT CGAAGTCACA GCAAGGAGAC TCCAGGGGCT 
GACACGCAAG TGCGCTGTGA GCGCAGTAGA CGTCACCACC GGCGCGGCTC CCCGGAGGAG 
GCCACTGAAC GGGAGCCTCG GCGCCACCGT GCCCACCGGC ATGCACAGGA CTCAAGCAAG 
GAGGGCACGG CGCCGGTGCT TGTACCCAAG GGTGAGCGAC GAGCAAGACA CCGAGGCCCA 
CGCACGGGTC CACGTGAGGC AGAGAACAAC GAGGAGCCCA CACGCAGGCA CCGTGCAAGG 
CATAAGGTGC CACCCACACT GCAGCCCCCA GAGAGGGAGG CTGCAGAGAA GGAGAGCAAC 
GCGGTGGAAG GGGATAAGGA AACCCGAAAT CACCAGCCCA AGGAACCTCA CTGTGACCTG 
GAGGCCATTG CAGTTACAGG TGTGGGCCCT CTGCACATGC TGCCCAGCAC CTGTCTCCAG 
AAAGTGGACG AGCAACCAGA GGATGCAGAC AACCAGCGTA ATGTCACCCG GATGGGCAGT 
CAGCCCTCAG ATCCCAGCAC CACTGTGCAT GTCCCAGTGA CACTGACAGG CCCTCCTGGG 
GAGACCCCTG TAGTTCCCAG TGGTAACATG AACCTGGAAG GCCAAGCAGA GGGCAAGAAG 
GAGGCAGAGG CGGATGATGT GCTGAGAAGA GGCCCCAGGC CCATCGTTCC CTACAGCTCC 
ATGTTTTGTC TCAGCCCCAC CAACCTGCTT CGTCGCTTCT GCCATTACAT TGTGACCATG 
CGGTACTTTG AGATGGTAAT TCTTGTGGTC ATTGCCTTGA GCAGCATTGC CCTGGCTGCA 
GAGGATCCTG TGCGGACAGA TTCATTCAGG AACAACGCTT TAAAGTACAT GGATTACATC 
TTTACAGGAG TCTTCACCTT TGAAATGGTC ATAAAGATGA TAGACTTGGG CTTGCTGCTG 
CACCCTGGTG CCTACTTCCG GGACCTGTGG AACATTCTGG ACTTCATTGT TGTCAGTGGA 
GCCCTGGTGG CATTTGCGTT CTCAGGATCC AAAGGGAAAG ACATCAATAC CATCAAGTCT 
CTGAGAGTCC TGCGTGTCCT GAGGCCCCTC AAGACCATCA AGCGGCTGCC TAAGCTCAAG 
GCTGTCTTTG ACTGTGTGGT GAACTCCCTG AAGAACGTCT TGAACATCCT GATTGTCTAC 
ATGCTCTTCA TGTTCATATT TGCCGTCATT GCCGTCCAGC TCTTCAAAGG GAAGTTCTTT 
TACTGTACTG ATGAATCCAA GGAGCTGGAG AGGGACTGCC GGGGTCAGTA TTTGGATTAT 
GAGAAGGAAG AAGTAGAAGC CCAGCCAAGG CAGTGGAAGA AATATGACTT CCACTATGAC 
AATGTTCTCT GGGCCTTGTT GACGCTGTTC ACAGTGTCCA CGGGAGAGGG GTGGCCCATG 
GTGCTGAAAC ACTCTGTGGA TGCCACCTAT GAGGAACAGG GGCCCAGTCC CGGGTTCCGG 
ATGGAGCTCT CCATCTTCTA CGTGGTCTAC TTTGTGGTCT TCCCTTTTTT CTTTGTCAAC 
ATCTTTGTGG CCTTGATCAT TATCACCTTC CAGGAACAGG GAGATAAGGT GATGTCTGAA 
TGCAGCTTAG AAAAGAATGA GAGGGCTTGC ATTGATTTTG CCATCAGTGC CAAGCCCCTG 
ACACGGTACA TGCCTCAAAA CAAACAGTCG TTCCAGTATA AGACATGGAC ATTCGTGGTC 
TCTCCACCCT TTGAGTACTT CATCATGGCT ATGATAGCCC TCAACACAGT GGTGCTGATG 
ATGAAGTTCT ATGATGCACC TTATGAGTAC GAGCTGATGC TGAAATGCCT GAACATTGTC 
TTCACATCCA TGTTCTCGAT GGAGTGCATA CTGAAGATCA TCGCCTTTGG GGTATTGAAC 
TACTTCAGAG ATGCCTGGAA CGTCTTTGAC TTTGTCACGG TTTTGGGAAG TATTACTGAT 
ATTTTAGTAA CAGAGATAGC GGAAACGAAC AACTTCATCA ACCTAAGCTT CCTTCGCCTC 
TTCCGGGCGG CACGGCTGAT CAAGCTGCTT CGCCAGGGCT ACACCATCCG CATCCTATTG 
TGGACCTTCG TCCAGTCCTT TAAGGCGCTG CCCTACGTGT GCCTCCTCAT TGCCATGCTG 
TTCTTCATCT ACGCCATCAT CGGCATGCAG GTTTTTGGAA ACATTGCCCT TGATGATGAC 
ACCAGTATCA ACCGACACAA CAACTTCCGG ACATTTCTGC AAGCCTTAAT GCTATTGTTC 
AGGAGTGCCA CTGGGGAGGC CTGGCATGAG ATCATGCTGT CTTGTCTGGG CAACCGGGCC 
TGTGACCCAC ATGCCAACGC CAGTGAGTGC GGGAGCGACT TTGCCTATTT TTATTTTGTC 
TCCTTCATCT TCCTCTGTTC CTTTCTGATG TTGAACCTCT TTGTTGCTGT AATCATGGAC 
AATTTTGAGT ACCTCACTCG GGACTCTTCC ATCCTAGGGC CTCACCACTT AGACGAATTC 
ATTCGAGTCT GGGCTGAATA CGACCCAGCT GCGTGTGGGC GCATCAGTTA CAATGACATG 
TTTGAGATGC TGAAACACAT GTCCCCACCT CTGGGGTTGG GGAAGAAATG CCCGGCTCGA 
GTTGCATACA AGCGCCTGGT TCGCATGAAC ATGCCCATAT CCAATGAGGA CATGACGGTG 
CACTTTACGT CCACACTGAT GGCCCTCATC CGGACAGCAC TGGAGATCAA GCTTGCCCCA 
GCGGGGACGA AGCAGCACCA GTGTGATGCT GAGCTGAGAA AAGAGATCTC TTCTGTGTGG 
GCTAATCTGC CCCAGAAGAC TCTGGACTTA CTGGTACCAC CCCACAAACC TGACGAGATG 
ACAGTGGGGA AGGTCTATGC TGCTCTCATG ATATTTGACT TCTACAAACA GAACAAAACC 
ACCAGAGATC AGACTCACCA AGCTCCCGGA GGCCTGTCCC AGATGGGTCC CGTTTCCCTG 
TTCCACCCTC TGAAGGCCAC CCTGGAACAG ACACAGCCCG CTGTGCTTCG AGGAGCTCGG 
GTTTTCCTTC GGCAAAAGAG TGCAACTTCC CTCAGCAATG GGGGTGCCAT ACAAACCCAG 
GAAAGTGGCA TCAAGGAGTC GCTGTCCTGG GGCACGCAGA GGACCCAAGA TGCACTTTAT 
GAGGCCAGAG CACCTCTAGA ACGTGGCCAT TCTGCAGAGA TCCCTGTGGG GCAGTCAGGA 
ACACTGGCTG TGGATGTCCA GATGCAGAAC ATGACACTGA GAGGACCAGA TGGGGAGCCC 
CAGCCTGGGC TGGAAAGCCA AGGCAGAGCT GCCTCTATGC CACGCCTAGC GGCAGAAACA 
CAGCCGGCCC CTAATGCCAG CCCCATGAAG CGCTCCATCT CCACACTGGC TCCACGCCCA 
CATGGGACTC AGCTTTGCAG CACAGTTCTG GACCGGCCTC CTCCTAGCCA GGCATCACAT 
CACCACCACC ACCGCTGCCA CCGGCGCAGA GACAAGAAGC AAAGGTCCCT GGAAAAGGGG 
CCCAGCCTGT CTGTTGACCC AGAAGGTGCA CCAAGCACTG CTGCAGGACC TGGTCTGCCC 
CATGGAGAAG GATCCACCGC CTGCCGGCGG GACCGTAAAC AGGAGCGAGG CCGGTCCCAG 
GAGCGGAGGC AGCCCTCATC TTCCTCTTCA GAGAAGCAGC GCTTCTATTC CTGTGACCGC 
TTTGGGAGCC GGGAGCCCCC GCAACTGATG CCCTCACTCA GTAGCCACCC CACATCGCCA 
ACAGCGGCGC TAGAGCCAGC ACCCCACCCA CAGGGCAGTG GTTCCGTTAA TGGGAGCCCC 
TTGATGTCAA CATCCGGTGC TAGCACCCCG GGCCGAGGTG GGCGGAGGCA GCTCCCCCAG 
ACTCCTCTGA CCCCACGCCC CAGCATCACC TACAAGACCG CCAATTCCTC GCCTGTCCAC 
TTTGCTGAGG GTCAGAGCGG CCTCCCAGCC TTCTCCCCTG GCCGTCTCAG CCGCGGCCTT 
TCTGAACACA ATGCCCTGCT CCAGAAAGAG CCCCTGAGCC AGCCTCTAGC TCCTGGCTCC 
CGAATTGGCT CTGACCCTTA CCTAGGGCAG CGTCTGGACA GTGAGGCCTC CGCCCACACC 
CTGCCTGAGG ATACACTCAC CTTTGAAGAG GCAGTGGCCA CCAACTCTGG CCGCTCCTCC 
AGGACTTCCT ATGTGTCCTC CCTCACTTCC CAATCCCACC CTCTCCGCCG TGTACCCAAT 
GGCTATCACT GCACTTTGGG ACTCAGCACT GGCGTCCGGG CGCGGCACAG CTACCACCAC 
CCCGATCAGG ACCACTGGTG CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cacna1b ( XM_006497629.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006497692.1 Mus musculus calcium channel, voltage-dependent, N type, alpha 1B subunit (Cacna1b), transcript variant X6, mRNA.

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CloneID	OMu29260
Clone ID Related Accession (Same CDS sequence)	XM_006497629.3
Accession Version	XM_006497629.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7041bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	voltage-dependent N-type calcium channel subunit alpha-1B isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006497629.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTCCGCT TCGGGGACGA GCTAGGCGGC CGCTATGGGG GCACCGGCGG CGGGGAGCGG 
GCTCGGGGTG GCGGGGCCGG CGGGGCGGGT GGCCCGGGCC AGGGGGGTCT GCCGCCGGGC 
CAGCGGGTCC TGTACAAGCA GTCCATTGCG CAGCGCGCAC GGACTATGGC CCTGTACAAC 
CCCATCCCAG TCAAGCAGAA CTGCTTCACC GTCAACCGCT CGCTCTTCGT CTTCAGCGAG 
GACAACGTCG TCCGCAAATA CGCTAAGCGC ATCACCGAAT GGCCGCCCTT CGAATACATG 
ATCCTGGCCA CCATCATCGC CAACTGCATT GTTCTGGCCC TGGAGCAGCA CCTCCCTGAT 
GGGGACAAGA CTCCCATGTC TGAGCGACTA GATGACACGG AGCCTTACTT CATCGGGATC 
TTTTGCTTCG AGGCGGGCAT CAAGATCATA GCCCTGGGCT TTGTTTTCCA CAAGGGCTCC 
TACCTTCGGA ACGGCTGGAA TGTCATGGAC TTCGTGGTGG TCCTCACGGG GATTCTCGCC 
ACAGCTGGAA CTGACTTTGA CCTGCGCACA CTGAGGGCTG TGCGTGTGCT TAGGCCCCTG 
AAGCTGGTGT CTGGAATTCC AAGCTTGCAG GTGGTGCTTA AGTCCATCAT GAAGGCCATG 
GTCCCGCTGC TGCAGATTGG GCTGCTGCTC TTCTTTGCCA TCCTCATGTT TGCCATCATC 
GGCCTCGAAT TCTATATGGG CAAATTCCAT AAGGCCTGTT TCCCCAACAG CACAGATACA 
GAGCCTGTGG GTGACTTTCC CTGTGGCAAA GATCCCCCTG CTCGTCAGTG TGATGGTGAC 
ACCGAATGCC GGGAGTACTG GCCAGGACCC AACTTTGGCA TCACCAATTT TGACAACATC 
CTGTTTGCCA TCTTGACAGT GTTCCAGTGT ATCACCATGG AGGGCTGGAC TGACATCCTC 
TACAATACAA ATGATGCGGC TGGCAACACA TGGAACTGGT TGTACTTCAT CCCCCTCATC 
ATCATTGGCT CCTTCTTCAT GCTCAACCTG GTGCTGGGTG TGCTTTCCGG AGAGTTTGCC 
AAGGAGCGGG AGCGAGTCGA GAACCGCCGC GCCTTCCTGA AGCTCCGCAG GCAGCAGCAG 
ATTGAGCGAG AGCTGAATGG GTACTTGGAG TGGATCTTCA AGGCAGAGGA AGTCATGTTG 
GCAGAGGAGG ACAAGAATGC AGAAGAGAAA TCCCCTTTGG ATGCAGTGTT GAAGAGAGCT 
GCCACCAAGA AGAGCCGAAA TGACCTCATC CATGCAGAAG AGGGGGAGGA CCGGTTTGTA 
GACCTCTGTG CAGTTGGGTC TCCATTTGCT CGTGCCAGCC TCAAGAGTGG GAAGACGGAG 
AGCTCATCGT ACTTCCGGAG AAAGGAGAAG ATGTTCCGGT TCTTTATCCG GCGTATGGTG 
AAAGCACAGA GCTTCTACTG GGTGGTACTG TGTGTGGTGG CCCTGAACAC ACTGTGTGTG 
GCCATGGTGC ACTATAATCA GCCTCAGCGG CTTACCACTG CACTGTACTT TGCAGAGTTT 
GTTTTCCTGG GTCTCTTCCT CACAGAGATG TCCCTGAAGA TGTATGGCCT AGGGCCCAGA 
AGTTACTTCA GGTCTTCCTT CAACTGCTTT GACTTTGGGG TGATTGTGGG GAGTATCTTT 
GAAGTAGTCT GGGCTGCCAT CAAGCCAGGA ACCTCCTTTG GAATCAGTGT GCTGCGGGCT 
CTGCGACTGC TGAGGATATT CAAAGTTACC AAGTATTGGA ACTCTCTGAG GAACCTGGTG 
GTTTCCCTCC TCAATTCCAT GAAGTCCATC ATCAGCCTTC TCTTCCTGCT TTTCCTCTTC 
ATCGTGGTCT TCGCTCTGTT GGGGATGCAG CTGTTTGGGG GACAGTTCAA CTTTCAAGAT 
GAGACTCCAA CCACCAATTT TGATACCTTC CCAGCTGCCA TCCTCACTGT CTTTCAGATC 
CTGACAGGAG AGGATTGGAA TGCCGTAATG TATCATGGGA TTGAGTCGCA AGGTGGAGTC 
AGCAAAGGCA TGTTTTCTTC CTTTTACTTC ATCGTCCTGA CACTGTTTGG AAACTACACC 
CTGCTGAATG TTTTTCTGGC CATTGCTGTG GACAACCTTG CCAATGCCCA GGAGTTGACC 
AAGGATGAAG AGGAGATGGA AGAAGCAGCC AATCAGAAAC TTGCTCTTCA GAAGGCCAAA 
GAAGTAGCTG AAGTCAGCCC CATGTCTGCT GCCAATATCT CCATCGCTGC TTTTGTAAAG 
CAAACTCGAG GTACTGTATC TCGCAGCTCA TCTGTCTCCA GCGTAAACTC ACCGCAGCAG 
AACTCGGCCA AGGCGCGCTC AGTATGGGAG CAGCGGGCCA GTCAGCTAAG GCTCCAGAAT 
CTGCGTGCCA GCTGTGAGGC ATTGTACAGT GAGATGGACC CTGAGGAGCG CCTGCGTTAT 
GCCAGCACGC GCCATGTGAG GCCAGACATG AAGACACACA TGGACCGACC CCTAGTGGTG 
GAGCCTGGTC GAGATGGCTT GCGGGGACCC GTTGGGAGCA AGTCAAAGCC TGAAGGCACG 
GAGGCCACAG AAAGCGCGGA CCTACCTCGC AGGCACCACC GGCACCGTGA TAGGGACAAG 
ACCTCAGCCA CAGCACCTGC TGGAGGCGAA CAGGACAGGA CAGAAAGCAC CGAGACCGGG 
GCCCGGGAGG AACGTGCGCG CCCTCGTCGA AGTCACAGCA AGGAGACTCC AGGGGCTGAC 
ACGCAAGTGC GCTGTGAGCG CAGTAGACGT CACCACCGGC GCGGCTCCCC GGAGGAGGCC 
ACTGAACGGG AGCCTCGGCG CCACCGTGCC CACCGGCATG CACAGGACTC AAGCAAGGAG 
GGCACGGCGC CGGTGCTTGT ACCCAAGGGT GAGCGACGAG CAAGACACCG AGGCCCACGC 
ACGGGTCCAC GTGAGGCAGA GAACAACGAG GAGCCCACAC GCAGGCACCG TGCAAGGCAT 
AAGGTGCCAC CCACACTGCA GCCCCCAGAG AGGGAGGCTG CAGAGAAGGA GAGCAACGCG 
GTGGAAGGGG ATAAGGAAAC CCGAAATCAC CAGCCCAAGG AACCTCACTG TGACCTGGAG 
GCCATTGCAG TTACAGGTGT GGGCCCTCTG CACATGCTGC CCAGCACCTG TCTCCAGAAA 
GTGGACGAGC AACCAGAGGA TGCAGACAAC CAGCGTAATG TCACCCGGAT GGGCAGTCAG 
CCCTCAGATC CCAGCACCAC TGTGCATGTC CCAGTGACAC TGACAGGCCC TCCTGGGGAG 
ACCCCTGTAG TTCCCAGTGG TAACATGAAC CTGGAAGGCC AAGCAGAGGG CAAGAAGGAG 
GCAGAGGCGG ATGATGTGCT GAGAAGAGGC CCCAGGCCCA TCGTTCCCTA CAGCTCCATG 
TTTTGTCTCA GCCCCACCAA CCTGCTTCGT CGCTTCTGCC ATTACATTGT GACCATGCGG 
TACTTTGAGA TGGTAATTCT TGTGGTCATT GCCTTGAGCA GCATTGCCCT GGCTGCAGAG 
GATCCTGTGC GGACAGATTC ATTCAGGAAC AACGCTTTAA AGTACATGGA TTACATCTTT 
ACAGGAGTCT TCACCTTTGA AATGGTCATA AAGATGATAG ACTTGGGCTT GCTGCTGCAC 
CCTGGTGCCT ACTTCCGGGA CCTGTGGAAC ATTCTGGACT TCATTGTTGT CAGTGGAGCC 
CTGGTGGCAT TTGCGTTCTC AGGATCCAAA GGGAAAGACA TCAATACCAT CAAGTCTCTG 
AGAGTCCTGC GTGTCCTGAG GCCCCTCAAG ACCATCAAGC GGCTGCCTAA GCTCAAGGCT 
GTCTTTGACT GTGTGGTGAA CTCCCTGAAG AACGTCTTGA ACATCCTGAT TGTCTACATG 
CTCTTCATGT TCATATTTGC CGTCATTGCC GTCCAGCTCT TCAAAGGGAA GTTCTTTTAC 
TGTACTGATG AATCCAAGGA GCTGGAGAGG GACTGCCGGG GTCAGTATTT GGATTATGAG 
AAGGAAGAAG TAGAAGCCCA GCCAAGGCAG TGGAAGAAAT ATGACTTCCA CTATGACAAT 
GTTCTCTGGG CCTTGTTGAC GCTGTTCACA GTGTCCACGG GAGAGGGGTG GCCCATGGTG 
CTGAAACACT CTGTGGATGC CACCTATGAG GAACAGGGGC CCAGTCCCGG GTTCCGGATG 
GAGCTCTCCA TCTTCTACGT GGTCTACTTT GTGGTCTTCC CTTTTTTCTT TGTCAACATC 
TTTGTGGCCT TGATCATTAT CACCTTCCAG GAACAGGGAG ATAAGGTGAT GTCTGAATGC 
AGCTTAGAAA AGAATGAGAG GGCTTGCATT GATTTTGCCA TCAGTGCCAA GCCCCTGACA 
CGGTACATGC CTCAAAACAA ACAGTCGTTC CAGTATAAGA CATGGACATT CGTGGTCTCT 
CCACCCTTTG AGTACTTCAT CATGGCTATG ATAGCCCTCA ACACAGTGGT GCTGATGATG 
AAGTTCTATG ATGCACCTTA TGAGTACGAG CTGATGCTGA AATGCCTGAA CATTGTCTTC 
ACATCCATGT TCTCGATGGA GTGCATACTG AAGATCATCG CCTTTGGGGT ATTGAACTAC 
TTCAGAGATG CCTGGAACGT CTTTGACTTT GTCACGGTTT TGGGAAGTAT TACTGATATT 
TTAGTAACAG AGATAGCGGA AACGAACAAC TTCATCAACC TAAGCTTCCT TCGCCTCTTC 
CGGGCGGCAC GGCTGATCAA GCTGCTTCGC CAGGGCTACA CCATCCGCAT CCTATTGTGG 
ACCTTCGTCC AGTCCTTTAA GGCGCTGCCC TACGTGTGCC TCCTCATTGC CATGCTGTTC 
TTCATCTACG CCATCATCGG CATGCAGGTT TTTGGAAACA TTGCCCTTGA TGATGACACC 
AGTATCAACC GACACAACAA CTTCCGGACA TTTCTGCAAG CCTTAATGCT ATTGTTCAGG 
AGTGCCACTG GGGAGGCCTG GCATGAGATC ATGCTGTCTT GTCTGGGCAA CCGGGCCTGT 
GACCCACATG CCAACGCCAG TGAGTGCGGG AGCGACTTTG CCTATTTTTA TTTTGTCTCC 
TTCATCTTCC TCTGTTCCTT TCTGATGTTG AACCTCTTTG TTGCTGTAAT CATGGACAAT 
TTTGAGTACC TCACTCGGGA CTCTTCCATC CTAGGGCCTC ACCACTTAGA CGAATTCATT 
CGAGTCTGGG CTGAATACGA CCCAGCTGCG TGTGGGCGCA TCAGTTACAA TGACATGTTT 
GAGATGCTGA AACACATGTC CCCACCTCTG GGGTTGGGGA AGAAATGCCC GGCTCGAGTT 
GCATACAAGC GCCTGGTTCG CATGAACATG CCCATATCCA ATGAGGACAT GACGGTGCAC 
TTTACGTCCA CACTGATGGC CCTCATCCGG ACAGCACTGG AGATCAAGCT TGCCCCAGCG 
GGGACGAAGC AGCACCAGTG TGATGCTGAG CTGAGAAAAG AGATCTCTTC TGTGTGGGCT 
AATCTGCCCC AGAAGACTCT GGACTTACTG GTACCACCCC ACAAACCTGA CGAGATGACA 
GTGGGGAAGG TCTATGCTGC TCTCATGATA TTTGACTTCT ACAAACAGAA CAAAACCACC 
AGAGATCAGA CTCACCAAGC TCCCGGAGGC CTGTCCCAGA TGGGTCCCGT TTCCCTGTTC 
CACCCTCTGA AGGCCACCCT GGAACAGACA CAGCCCGCTG TGCTTCGAGG AGCTCGGGTT 
TTCCTTCGGC AAAAGAGTGC AACTTCCCTC AGCAATGGGG GTGCCATACA AACCCAGGAA 
AGTGGCATCA AGGAGTCGCT GTCCTGGGGC ACGCAGAGGA CCCAAGATGC ACTTTATGAG 
GCCAGAGCAC CTCTAGAACG TGGCCATTCT GCAGAGATCC CTGTGGGGCA GTCAGGAACA 
CTGGCTGTGG ATGTCCAGAT GCAGAACATG ACACTGAGAG GACCAGATGG GGAGCCCCAG 
CCTGGGCTGG AAAGCCAAGG CAGAGCTGCC TCTATGCCAC GCCTAGCGGC AGAAACACAG 
CCGGCCCCTA ATGCCAGCCC CATGAAGCGC TCCATCTCCA CACTGGCTCC ACGCCCACAT 
GGGACTCAGC TTTGCAGCAC AGTTCTGGAC CGGCCTCCTC CTAGCCAGGC ATCACATCAC 
CACCACCACC GCTGCCACCG GCGCAGAGAC AAGAAGCAAA GGTCCCTGGA AAAGGGGCCC 
AGCCTGTCTG TTGACCCAGA AGGTGCACCA AGCACTGCTG CAGGACCTGG TCTGCCCCAT 
GGAGAAGGAT CCACCGCCTG CCGGCGGGAC CGTAAACAGG AGCGAGGCCG GTCCCAGGAG 
CGGAGGCAGC CCTCATCTTC CTCTTCAGAG AAGCAGCGCT TCTATTCCTG TGACCGCTTT 
GGGAGCCGGG AGCCCCCGCA ACTGATGCCC TCACTCAGTA GCCACCCCAC ATCGCCAACA 
GCGGCGCTAG AGCCAGCACC CCACCCACAG GGCAGTGGTT CCGTTAATGG GAGCCCCTTG 
ATGTCAACAT CCGGTGCTAG CACCCCGGGC CGAGGTGGGC GGAGGCAGCT CCCCCAGACT 
CCTCTGACCC CACGCCCCAG CATCACCTAC AAGACCGCCA ATTCCTCGCC TGTCCACTTT 
GCTGAGGGTC AGAGCGGCCT CCCAGCCTTC TCCCCTGGCC GTCTCAGCCG CGGCCTTTCT 
GAACACAATG CCCTGCTCCA GAAAGAGCCC CTGAGCCAGC CTCTAGCTCC TGGCTCCCGA 
ATTGGCTCTG ACCCTTACCT AGGGCAGCGT CTGGACAGTG AGGCCTCCGC CCACACCCTG 
CCTGAGGATA CACTCACCTT TGAAGAGGCA GTGGCCACCA ACTCTGGCCG CTCCTCCAGG 
ACTTCCTATG TGTCCTCCCT CACTTCCCAA TCCCACCCTC TCCGCCGTGT ACCCAATGGC 
TATCACTGCA CTTTGGGACT CAGCACTGGC GTCCGGGCGC GGCACAGCTA CCACCACCCC 
GATCAGGACC ACTGGTGCTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006497692.1
CDS	146..7186
Translation	MVRFGDELGGRYGGTGGGERARGGGAGGAGGPGQGGLPPGQRVLYKQSIAQRARTMALYNPIPVKQNCFTVNRSLFVFSEDNVVRKYAKRITEWPPFEYMILATIIANCIVLALEQHLPDGDKTPMSERLDDTEPYFIGIFCFEAGIKIIALGFVFHKGSYLRNGWNVMDFVVVLTGILATAGTDFDLRTLRAVRVLRPLKLVSGIPSLQVVLKSIMKAMVPLLQIGLLLFFAILMFAIIGLEFYMGKFHKACFPNSTDTEPVGDFPCGKDPPARQCDGDTECREYWPGPNFGITNFDNILFAILTVFQCITMEGWTDILYNTNDAAGNTWNWLYFIPLIIIGSFFMLNLVLGVLSGEFAKERERVENRRAFLKLRRQQQIERELNGYLEWIFKAEEVMLAEEDKNAEEKSPLDAVLKRAATKKSRNDLIHAEEGEDRFVDLCAVGSPFARASLKSGKTESSSYFRRKEKMFRFFIRRMVKAQSFYWVVLCVVALNTLCVAMVHYNQPQRLTTALYFAEFVFLGLFLTEMSLKMYGLGPRSYFRSSFNCFDFGVIVGSIFEVVWAAIKPGTSFGISVLRALRLLRIFKVTKYWNSLRNLVVSLLNSMKSIISLLFLLFLFIVVFALLGMQLFGGQFNFQDETPTTNFDTFPAAILTVFQILTGEDWNAVMYHGIESQGGVSKGMFSSFYFIVLTLFGNYTLLNVFLAIAVDNLANAQELTKDEEEMEEAANQKLALQKAKEVAEVSPMSAANISIAAFVKQTRGTVSRSSSVSSVNSPQQNSAKARSVWEQRASQLRLQNLRASCEALYSEMDPEERLRYASTRHVRPDMKTHMDRPLVVEPGRDGLRGPVGSKSKPEGTEATESADLPRRHHRHRDRDKTSATAPAGGEQDRTESTETGAREERARPRRSHSKETPGADTQVRCERSRRHHRRGSPEEATEREPRRHRAHRHAQDSSKEGTAPVLVPKGERRARHRGPRTGPREAENNEEPTRRHRARHKVPPTLQPPEREAAEKESNAVEGDKETRNHQPKEPHCDLEAIAVTGVGPLHMLPSTCLQKVDEQPEDADNQRNVTRMGSQPSDPSTTVHVPVTLTGPPGETPVVPSGNMNLEGQAEGKKEAEADDVLRRGPRPIVPYSSMFCLSPTNLLRRFCHYIVTMRYFEMVILVVIALSSIALAAEDPVRTDSFRNNALKYMDYIFTGVFTFEMVIKMIDLGLLLHPGAYFRDLWNILDFIVVSGALVAFAFSGSKGKDINTIKSLRVLRVLRPLKTIKRLPKLKAVFDCVVNSLKNVLNILIVYMLFMFIFAVIAVQLFKGKFFYCTDESKELERDCRGQYLDYEKEEVEAQPRQWKKYDFHYDNVLWALLTLFTVSTGEGWPMVLKHSVDATYEEQGPSPGFRMELSIFYVVYFVVFPFFFVNIFVALIIITFQEQGDKVMSECSLEKNERACIDFAISAKPLTRYMPQNKQSFQYKTWTFVVSPPFEYFIMAMIALNTVVLMMKFYDAPYEYELMLKCLNIVFTSMFSMECILKIIAFGVLNYFRDAWNVFDFVTVLGSITDILVTEIAETNNFINLSFLRLFRAARLIKLLRQGYTIRILLWTFVQSFKALPYVCLLIAMLFFIYAIIGMQVFGNIALDDDTSINRHNNFRTFLQALMLLFRSATGEAWHEIMLSCLGNRACDPHANASECGSDFAYFYFVSFIFLCSFLMLNLFVAVIMDNFEYLTRDSSILGPHHLDEFIRVWAEYDPAACGRISYNDMFEMLKHMSPPLGLGKKCPARVAYKRLVRMNMPISNEDMTVHFTSTLMALIRTALEIKLAPAGTKQHQCDAELRKEISSVWANLPQKTLDLLVPPHKPDEMTVGKVYAALMIFDFYKQNKTTRDQTHQAPGGLSQMGPVSLFHPLKATLEQTQPAVLRGARVFLRQKSATSLSNGGAIQTQESGIKESLSWGTQRTQDALYEARAPLERGHSAEIPVGQSGTLAVDVQMQNMTLRGPDGEPQPGLESQGRAASMPRLAAETQPAPNASPMKRSISTLAPRPHGTQLCSTVLDRPPPSQASHHHHHRCHRRRDKKQRSLEKGPSLSVDPEGAPSTAAGPGLPHGEGSTACRRDRKQERGRSQERRQPSSSSSEKQRFYSCDRFGSREPPQLMPSLSSHPTSPTAALEPAPHPQGSGSVNGSPLMSTSGASTPGRGGRRQLPQTPLTPRPSITYKTANSSPVHFAEGQSGLPAFSPGRLSRGLSEHNALLQKEPLSQPLAPGSRIGSDPYLGQRLDSEASAHTLPEDTLTFEEAVATNSGRSSRTSYVSSLTSQSHPLRRVPNGYHCTLGLSTGVRARHSYHHPDQDHWC

Target ORF information:

RefSeq Version	XM_006497629.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus calcium channel, voltage-dependent, N type, alpha 1B subunit (Cacna1b), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006497629.3

ORF Insert Sequence:

ATGGTCCGCT TCGGGGACGA GCTAGGCGGC CGCTATGGGG GCACCGGCGG CGGGGAGCGG 
GCTCGGGGTG GCGGGGCCGG CGGGGCGGGT GGCCCGGGCC AGGGGGGTCT GCCGCCGGGC 
CAGCGGGTCC TGTACAAGCA GTCCATTGCG CAGCGCGCAC GGACTATGGC CCTGTACAAC 
CCCATCCCAG TCAAGCAGAA CTGCTTCACC GTCAACCGCT CGCTCTTCGT CTTCAGCGAG 
GACAACGTCG TCCGCAAATA CGCTAAGCGC ATCACCGAAT GGCCGCCCTT CGAATACATG 
ATCCTGGCCA CCATCATCGC CAACTGCATT GTTCTGGCCC TGGAGCAGCA CCTCCCTGAT 
GGGGACAAGA CTCCCATGTC TGAGCGACTA GATGACACGG AGCCTTACTT CATCGGGATC 
TTTTGCTTCG AGGCGGGCAT CAAGATCATA GCCCTGGGCT TTGTTTTCCA CAAGGGCTCC 
TACCTTCGGA ACGGCTGGAA TGTCATGGAC TTCGTGGTGG TCCTCACGGG GATTCTCGCC 
ACAGCTGGAA CTGACTTTGA CCTGCGCACA CTGAGGGCTG TGCGTGTGCT TAGGCCCCTG 
AAGCTGGTGT CTGGAATTCC AAGCTTGCAG GTGGTGCTTA AGTCCATCAT GAAGGCCATG 
GTCCCGCTGC TGCAGATTGG GCTGCTGCTC TTCTTTGCCA TCCTCATGTT TGCCATCATC 
GGCCTCGAAT TCTATATGGG CAAATTCCAT AAGGCCTGTT TCCCCAACAG CACAGATACA 
GAGCCTGTGG GTGACTTTCC CTGTGGCAAA GATCCCCCTG CTCGTCAGTG TGATGGTGAC 
ACCGAATGCC GGGAGTACTG GCCAGGACCC AACTTTGGCA TCACCAATTT TGACAACATC 
CTGTTTGCCA TCTTGACAGT GTTCCAGTGT ATCACCATGG AGGGCTGGAC TGACATCCTC 
TACAATACAA ATGATGCGGC TGGCAACACA TGGAACTGGT TGTACTTCAT CCCCCTCATC 
ATCATTGGCT CCTTCTTCAT GCTCAACCTG GTGCTGGGTG TGCTTTCCGG AGAGTTTGCC 
AAGGAGCGGG AGCGAGTCGA GAACCGCCGC GCCTTCCTGA AGCTCCGCAG GCAGCAGCAG 
ATTGAGCGAG AGCTGAATGG GTACTTGGAG TGGATCTTCA AGGCAGAGGA AGTCATGTTG 
GCAGAGGAGG ACAAGAATGC AGAAGAGAAA TCCCCTTTGG ATGCAGTGTT GAAGAGAGCT 
GCCACCAAGA AGAGCCGAAA TGACCTCATC CATGCAGAAG AGGGGGAGGA CCGGTTTGTA 
GACCTCTGTG CAGTTGGGTC TCCATTTGCT CGTGCCAGCC TCAAGAGTGG GAAGACGGAG 
AGCTCATCGT ACTTCCGGAG AAAGGAGAAG ATGTTCCGGT TCTTTATCCG GCGTATGGTG 
AAAGCACAGA GCTTCTACTG GGTGGTACTG TGTGTGGTGG CCCTGAACAC ACTGTGTGTG 
GCCATGGTGC ACTATAATCA GCCTCAGCGG CTTACCACTG CACTGTACTT TGCAGAGTTT 
GTTTTCCTGG GTCTCTTCCT CACAGAGATG TCCCTGAAGA TGTATGGCCT AGGGCCCAGA 
AGTTACTTCA GGTCTTCCTT CAACTGCTTT GACTTTGGGG TGATTGTGGG GAGTATCTTT 
GAAGTAGTCT GGGCTGCCAT CAAGCCAGGA ACCTCCTTTG GAATCAGTGT GCTGCGGGCT 
CTGCGACTGC TGAGGATATT CAAAGTTACC AAGTATTGGA ACTCTCTGAG GAACCTGGTG 
GTTTCCCTCC TCAATTCCAT GAAGTCCATC ATCAGCCTTC TCTTCCTGCT TTTCCTCTTC 
ATCGTGGTCT TCGCTCTGTT GGGGATGCAG CTGTTTGGGG GACAGTTCAA CTTTCAAGAT 
GAGACTCCAA CCACCAATTT TGATACCTTC CCAGCTGCCA TCCTCACTGT CTTTCAGATC 
CTGACAGGAG AGGATTGGAA TGCCGTAATG TATCATGGGA TTGAGTCGCA AGGTGGAGTC 
AGCAAAGGCA TGTTTTCTTC CTTTTACTTC ATCGTCCTGA CACTGTTTGG AAACTACACC 
CTGCTGAATG TTTTTCTGGC CATTGCTGTG GACAACCTTG CCAATGCCCA GGAGTTGACC 
AAGGATGAAG AGGAGATGGA AGAAGCAGCC AATCAGAAAC TTGCTCTTCA GAAGGCCAAA 
GAAGTAGCTG AAGTCAGCCC CATGTCTGCT GCCAATATCT CCATCGCTGC TTTTGTAAAG 
CAAACTCGAG GTACTGTATC TCGCAGCTCA TCTGTCTCCA GCGTAAACTC ACCGCAGCAG 
AACTCGGCCA AGGCGCGCTC AGTATGGGAG CAGCGGGCCA GTCAGCTAAG GCTCCAGAAT 
CTGCGTGCCA GCTGTGAGGC ATTGTACAGT GAGATGGACC CTGAGGAGCG CCTGCGTTAT 
GCCAGCACGC GCCATGTGAG GCCAGACATG AAGACACACA TGGACCGACC CCTAGTGGTG 
GAGCCTGGTC GAGATGGCTT GCGGGGACCC GTTGGGAGCA AGTCAAAGCC TGAAGGCACG 
GAGGCCACAG AAAGCGCGGA CCTACCTCGC AGGCACCACC GGCACCGTGA TAGGGACAAG 
ACCTCAGCCA CAGCACCTGC TGGAGGCGAA CAGGACAGGA CAGAAAGCAC CGAGACCGGG 
GCCCGGGAGG AACGTGCGCG CCCTCGTCGA AGTCACAGCA AGGAGACTCC AGGGGCTGAC 
ACGCAAGTGC GCTGTGAGCG CAGTAGACGT CACCACCGGC GCGGCTCCCC GGAGGAGGCC 
ACTGAACGGG AGCCTCGGCG CCACCGTGCC CACCGGCATG CACAGGACTC AAGCAAGGAG 
GGCACGGCGC CGGTGCTTGT ACCCAAGGGT GAGCGACGAG CAAGACACCG AGGCCCACGC 
ACGGGTCCAC GTGAGGCAGA GAACAACGAG GAGCCCACAC GCAGGCACCG TGCAAGGCAT 
AAGGTGCCAC CCACACTGCA GCCCCCAGAG AGGGAGGCTG CAGAGAAGGA GAGCAACGCG 
GTGGAAGGGG ATAAGGAAAC CCGAAATCAC CAGCCCAAGG AACCTCACTG TGACCTGGAG 
GCCATTGCAG TTACAGGTGT GGGCCCTCTG CACATGCTGC CCAGCACCTG TCTCCAGAAA 
GTGGACGAGC AACCAGAGGA TGCAGACAAC CAGCGTAATG TCACCCGGAT GGGCAGTCAG 
CCCTCAGATC CCAGCACCAC TGTGCATGTC CCAGTGACAC TGACAGGCCC TCCTGGGGAG 
ACCCCTGTAG TTCCCAGTGG TAACATGAAC CTGGAAGGCC AAGCAGAGGG CAAGAAGGAG 
GCAGAGGCGG ATGATGTGCT GAGAAGAGGC CCCAGGCCCA TCGTTCCCTA CAGCTCCATG 
TTTTGTCTCA GCCCCACCAA CCTGCTTCGT CGCTTCTGCC ATTACATTGT GACCATGCGG 
TACTTTGAGA TGGTAATTCT TGTGGTCATT GCCTTGAGCA GCATTGCCCT GGCTGCAGAG 
GATCCTGTGC GGACAGATTC ATTCAGGAAC AACGCTTTAA AGTACATGGA TTACATCTTT 
ACAGGAGTCT TCACCTTTGA AATGGTCATA AAGATGATAG ACTTGGGCTT GCTGCTGCAC 
CCTGGTGCCT ACTTCCGGGA CCTGTGGAAC ATTCTGGACT TCATTGTTGT CAGTGGAGCC 
CTGGTGGCAT TTGCGTTCTC AGGATCCAAA GGGAAAGACA TCAATACCAT CAAGTCTCTG 
AGAGTCCTGC GTGTCCTGAG GCCCCTCAAG ACCATCAAGC GGCTGCCTAA GCTCAAGGCT 
GTCTTTGACT GTGTGGTGAA CTCCCTGAAG AACGTCTTGA ACATCCTGAT TGTCTACATG 
CTCTTCATGT TCATATTTGC CGTCATTGCC GTCCAGCTCT TCAAAGGGAA GTTCTTTTAC 
TGTACTGATG AATCCAAGGA GCTGGAGAGG GACTGCCGGG GTCAGTATTT GGATTATGAG 
AAGGAAGAAG TAGAAGCCCA GCCAAGGCAG TGGAAGAAAT ATGACTTCCA CTATGACAAT 
GTTCTCTGGG CCTTGTTGAC GCTGTTCACA GTGTCCACGG GAGAGGGGTG GCCCATGGTG 
CTGAAACACT CTGTGGATGC CACCTATGAG GAACAGGGGC CCAGTCCCGG GTTCCGGATG 
GAGCTCTCCA TCTTCTACGT GGTCTACTTT GTGGTCTTCC CTTTTTTCTT TGTCAACATC 
TTTGTGGCCT TGATCATTAT CACCTTCCAG GAACAGGGAG ATAAGGTGAT GTCTGAATGC 
AGCTTAGAAA AGAATGAGAG GGCTTGCATT GATTTTGCCA TCAGTGCCAA GCCCCTGACA 
CGGTACATGC CTCAAAACAA ACAGTCGTTC CAGTATAAGA CATGGACATT CGTGGTCTCT 
CCACCCTTTG AGTACTTCAT CATGGCTATG ATAGCCCTCA ACACAGTGGT GCTGATGATG 
AAGTTCTATG ATGCACCTTA TGAGTACGAG CTGATGCTGA AATGCCTGAA CATTGTCTTC 
ACATCCATGT TCTCGATGGA GTGCATACTG AAGATCATCG CCTTTGGGGT ATTGAACTAC 
TTCAGAGATG CCTGGAACGT CTTTGACTTT GTCACGGTTT TGGGAAGTAT TACTGATATT 
TTAGTAACAG AGATAGCGGA AACGAACAAC TTCATCAACC TAAGCTTCCT TCGCCTCTTC 
CGGGCGGCAC GGCTGATCAA GCTGCTTCGC CAGGGCTACA CCATCCGCAT CCTATTGTGG 
ACCTTCGTCC AGTCCTTTAA GGCGCTGCCC TACGTGTGCC TCCTCATTGC CATGCTGTTC 
TTCATCTACG CCATCATCGG CATGCAGGTT TTTGGAAACA TTGCCCTTGA TGATGACACC 
AGTATCAACC GACACAACAA CTTCCGGACA TTTCTGCAAG CCTTAATGCT ATTGTTCAGG 
AGTGCCACTG GGGAGGCCTG GCATGAGATC ATGCTGTCTT GTCTGGGCAA CCGGGCCTGT 
GACCCACATG CCAACGCCAG TGAGTGCGGG AGCGACTTTG CCTATTTTTA TTTTGTCTCC 
TTCATCTTCC TCTGTTCCTT TCTGATGTTG AACCTCTTTG TTGCTGTAAT CATGGACAAT 
TTTGAGTACC TCACTCGGGA CTCTTCCATC CTAGGGCCTC ACCACTTAGA CGAATTCATT 
CGAGTCTGGG CTGAATACGA CCCAGCTGCG TGTGGGCGCA TCAGTTACAA TGACATGTTT 
GAGATGCTGA AACACATGTC CCCACCTCTG GGGTTGGGGA AGAAATGCCC GGCTCGAGTT 
GCATACAAGC GCCTGGTTCG CATGAACATG CCCATATCCA ATGAGGACAT GACGGTGCAC 
TTTACGTCCA CACTGATGGC CCTCATCCGG ACAGCACTGG AGATCAAGCT TGCCCCAGCG 
GGGACGAAGC AGCACCAGTG TGATGCTGAG CTGAGAAAAG AGATCTCTTC TGTGTGGGCT 
AATCTGCCCC AGAAGACTCT GGACTTACTG GTACCACCCC ACAAACCTGA CGAGATGACA 
GTGGGGAAGG TCTATGCTGC TCTCATGATA TTTGACTTCT ACAAACAGAA CAAAACCACC 
AGAGATCAGA CTCACCAAGC TCCCGGAGGC CTGTCCCAGA TGGGTCCCGT TTCCCTGTTC 
CACCCTCTGA AGGCCACCCT GGAACAGACA CAGCCCGCTG TGCTTCGAGG AGCTCGGGTT 
TTCCTTCGGC AAAAGAGTGC AACTTCCCTC AGCAATGGGG GTGCCATACA AACCCAGGAA 
AGTGGCATCA AGGAGTCGCT GTCCTGGGGC ACGCAGAGGA CCCAAGATGC ACTTTATGAG 
GCCAGAGCAC CTCTAGAACG TGGCCATTCT GCAGAGATCC CTGTGGGGCA GTCAGGAACA 
CTGGCTGTGG ATGTCCAGAT GCAGAACATG ACACTGAGAG GACCAGATGG GGAGCCCCAG 
CCTGGGCTGG AAAGCCAAGG CAGAGCTGCC TCTATGCCAC GCCTAGCGGC AGAAACACAG 
CCGGCCCCTA ATGCCAGCCC CATGAAGCGC TCCATCTCCA CACTGGCTCC ACGCCCACAT 
GGGACTCAGC TTTGCAGCAC AGTTCTGGAC CGGCCTCCTC CTAGCCAGGC ATCACATCAC 
CACCACCACC GCTGCCACCG GCGCAGAGAC AAGAAGCAAA GGTCCCTGGA AAAGGGGCCC 
AGCCTGTCTG TTGACCCAGA AGGTGCACCA AGCACTGCTG CAGGACCTGG TCTGCCCCAT 
GGAGAAGGAT CCACCGCCTG CCGGCGGGAC CGTAAACAGG AGCGAGGCCG GTCCCAGGAG 
CGGAGGCAGC CCTCATCTTC CTCTTCAGAG AAGCAGCGCT TCTATTCCTG TGACCGCTTT 
GGGAGCCGGG AGCCCCCGCA ACTGATGCCC TCACTCAGTA GCCACCCCAC ATCGCCAACA 
GCGGCGCTAG AGCCAGCACC CCACCCACAG GGCAGTGGTT CCGTTAATGG GAGCCCCTTG 
ATGTCAACAT CCGGTGCTAG CACCCCGGGC CGAGGTGGGC GGAGGCAGCT CCCCCAGACT 
CCTCTGACCC CACGCCCCAG CATCACCTAC AAGACCGCCA ATTCCTCGCC TGTCCACTTT 
GCTGAGGGTC AGAGCGGCCT CCCAGCCTTC TCCCCTGGCC GTCTCAGCCG CGGCCTTTCT 
GAACACAATG CCCTGCTCCA GAAAGAGCCC CTGAGCCAGC CTCTAGCTCC TGGCTCCCGA 
ATTGGCTCTG ACCCTTACCT AGGGCAGCGT CTGGACAGTG AGGCCTCCGC CCACACCCTG 
CCTGAGGATA CACTCACCTT TGAAGAGGCA GTGGCCACCA ACTCTGGCCG CTCCTCCAGG 
ACTTCCTATG TGTCCTCCCT CACTTCCCAA TCCCACCCTC TCCGCCGTGT ACCCAATGGC 
TATCACTGCA CTTTGGGACT CAGCACTGGC GTCCGGGCGC GGCACAGCTA CCACCACCCC 
GATCAGGACC ACTGGTGCTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cacna1b ( XM_006497630.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006497693.1 Mus musculus calcium channel, voltage-dependent, N type, alpha 1B subunit (Cacna1b), transcript variant X7, mRNA.

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CloneID	OMu29261
Clone ID Related Accession (Same CDS sequence)	XM_006497630.3
Accession Version	XM_006497630.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7038bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	voltage-dependent N-type calcium channel subunit alpha-1B isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006497630.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTCCGCT TCGGGGACGA GCTAGGCGGC CGCTATGGGG GCACCGGCGG CGGGGAGCGG 
GCTCGGGGTG GCGGGGCCGG CGGGGCGGGT GGCCCGGGCC AGGGGGGTCT GCCGCCGGGC 
CAGCGGGTCC TGTACAAGCA GTCCATTGCG CAGCGCGCAC GGACTATGGC CCTGTACAAC 
CCCATCCCAG TCAAGCAGAA CTGCTTCACC GTCAACCGCT CGCTCTTCGT CTTCAGCGAG 
GACAACGTCG TCCGCAAATA CGCTAAGCGC ATCACCGAAT GGCCGCCCTT CGAATACATG 
ATCCTGGCCA CCATCATCGC CAACTGCATT GTTCTGGCCC TGGAGCAGCA CCTCCCTGAT 
GGGGACAAGA CTCCCATGTC TGAGCGACTA GATGACACGG AGCCTTACTT CATCGGGATC 
TTTTGCTTCG AGGCGGGCAT CAAGATCATA GCCCTGGGCT TTGTTTTCCA CAAGGGCTCC 
TACCTTCGGA ACGGCTGGAA TGTCATGGAC TTCGTGGTGG TCCTCACGGG GATTCTCGCC 
ACAGCTGGAA CTGACTTTGA CCTGCGCACA CTGAGGGCTG TGCGTGTGCT TAGGCCCCTG 
AAGCTGGTGT CTGGAATTCC AAGCTTGCAG GTGGTGCTTA AGTCCATCAT GAAGGCCATG 
GTCCCGCTGC TGCAGATTGG GCTGCTGCTC TTCTTTGCCA TCCTCATGTT TGCCATCATC 
GGCCTCGAAT TCTATATGGG CAAATTCCAT AAGGCCTGTT TCCCCAACAG CACAGATACA 
GAGCCTGTGG GTGACTTTCC CTGTGGCAAA GATCCCCCTG CTCGTCAGTG TGATGGTGAC 
ACCGAATGCC GGGAGTACTG GCCAGGACCC AACTTTGGCA TCACCAATTT TGACAACATC 
CTGTTTGCCA TCTTGACAGT GTTCCAGTGT ATCACCATGG AGGGCTGGAC TGACATCCTC 
TACAATACAA ATGATGCGGC TGGCAACACA TGGAACTGGT TGTACTTCAT CCCCCTCATC 
ATCATTGGCT CCTTCTTCAT GCTCAACCTG GTGCTGGGTG TGCTTTCCGG AGAGTTTGCC 
AAGGAGCGGG AGCGAGTCGA GAACCGCCGC GCCTTCCTGA AGCTCCGCAG GCAGCAGCAG 
ATTGAGCGAG AGCTGAATGG GTACTTGGAG TGGATCTTCA AGGCAGAGGA AGTCATGTTG 
GCAGAGGAGG ACAAGAATGC AGAAGAGAAA TCCCCTTTGG ATGCAGTGTT GAAGAGAGCT 
GCCACCAAGA AGAGCCGAAA TGACCTCATC CATGCAGAAG AGGGGGAGGA CCGGTTTGTA 
GACCTCTGTG CAGTTGGGTC TCCATTTGCT CGTGCCAGCC TCAAGAGTGG GAAGACGGAG 
AGCTCATCGT ACTTCCGGAG AAAGGAGAAG ATGTTCCGGT TCTTTATCCG GCGTATGGTG 
AAAGCACAGA GCTTCTACTG GGTGGTACTG TGTGTGGTGG CCCTGAACAC ACTGTGTGTG 
GCCATGGTGC ACTATAATCA GCCTCAGCGG CTTACCACTG CACTGTACTT TGCAGAGTTT 
GTTTTCCTGG GTCTCTTCCT CACAGAGATG TCCCTGAAGA TGTATGGCCT AGGGCCCAGA 
AGTTACTTCA GGTCTTCCTT CAACTGCTTT GACTTTGGGG TGATTGTGGG GAGTATCTTT 
GAAGTAGTCT GGGCTGCCAT CAAGCCAGGA ACCTCCTTTG GAATCAGTGT GCTGCGGGCT 
CTGCGACTGC TGAGGATATT CAAAGTTACC AAGTATTGGA ACTCTCTGAG GAACCTGGTG 
GTTTCCCTCC TCAATTCCAT GAAGTCCATC ATCAGCCTTC TCTTCCTGCT TTTCCTCTTC 
ATCGTGGTCT TCGCTCTGTT GGGGATGCAG CTGTTTGGGG GACAGTTCAA CTTTCAAGAT 
GAGACTCCAA CCACCAATTT TGATACCTTC CCAGCTGCCA TCCTCACTGT CTTTCAGATC 
CTGACAGGAG AGGATTGGAA TGCCGTAATG TATCATGGGA TTGAGTCGCA AGGTGGAGTC 
AGCAAAGGCA TGTTTTCTTC CTTTTACTTC ATCGTCCTGA CACTGTTTGG AAACTACACC 
CTGCTGAATG TTTTTCTGGC CATTGCTGTG GACAACCTTG CCAATGCCCA GGAGTTGACC 
AAGGATGAAG AGGAGATGGA AGAAGCAGCC AATCAGAAAC TTGCTCTTCA GAAGGCCAAA 
GAAGTAGCTG AAGTCAGCCC CATGTCTGCT GCCAATATCT CCATCGCTGC TTTTGTAAAG 
CAAACTCGAG GTACTGTATC TCGCAGCTCA TCTGTCTCCA GCGTAAACTC ACCCAGGCAG 
CAGAACTCGG CCAAGGCGCG CTCAGTATGG GAGCAGCGGG CCAGTCAGCT AAGGCTCCAG 
AATCTGCGTG CCAGCTGTGA GGCATTGTAC AGTGAGATGG ACCCTGAGGA GCGCCTGCGT 
TATGCCAGCA CGCGCCATGT GAGGCCAGAC ATGAAGACAC ACATGGACCG ACCCCTAGTG 
GTGGAGCCTG GTCGAGATGG CTTGCGGGGA CCCGTTGGGA GCAAGTCAAA GCCTGAAGGC 
ACGGAGGCCA CAGAAAGCGC GGACCTACCT CGCAGGCACC ACCGGCACCG TGATAGGGAC 
AAGACCTCAG CCACAGCACC TGCTGGAGGC GAACAGGACA GGACAGAAAG CACCGAGACC 
GGGGCCCGGG AGGAACGTGC GCGCCCTCGT CGAAGTCACA GCAAGGAGAC TCCAGGGGCT 
GACACGCAAG TGCGCTGTGA GCGCAGTAGA CGTCACCACC GGCGCGGCTC CCCGGAGGAG 
GCCACTGAAC GGGAGCCTCG GCGCCACCGT GCCCACCGGC ATGCACAGGA CTCAAGCAAG 
GAGGGCACGG CGCCGGTGCT TGTACCCAAG GGTGAGCGAC GAGCAAGACA CCGAGGCCCA 
CGCACGGGTC CACGTGAGGC AGAGAACAAC GAGGAGCCCA CACGCAGGCA CCGTGCAAGG 
CATAAGGTGC CACCCACACT GCAGCCCCCA GAGAGGGAGG CTGCAGAGAA GGAGAGCAAC 
GCGGTGGAAG GGGATAAGGA AACCCGAAAT CACCAGCCCA AGGAACCTCA CTGTGACCTG 
GAGGCCATTG CAGTTACAGG TGTGGGCCCT CTGCACATGC TGCCCAGCAC CTGTCTCCAG 
AAAGTGGACG AGCAACCAGA GGATGCAGAC AACCAGCGTA ATGTCACCCG GATGGGCAGT 
CAGCCCTCAG ATCCCAGCAC CACTGTGCAT GTCCCAGTGA CACTGACAGG CCCTCCTGGG 
GAGACCCCTG TAGTTCCCAG TGGTAACATG AACCTGGAAG GCCAAGCAGA GGGCAAGAAG 
GAGGCAGAGG CGGATGATGT GCTGAGAAGA GGCCCCAGGC CCATCGTTCC CTACAGCTCC 
ATGTTTTGTC TCAGCCCCAC CAACCTGCTT CGTCGCTTCT GCCATTACAT TGTGACCATG 
CGGTACTTTG AGATGGTAAT TCTTGTGGTC ATTGCCTTGA GCAGCATTGC CCTGGCTGCA 
GAGGATCCTG TGCGGACAGA TTCATTCAGG AACAACGCTT TAAAGTACAT GGATTACATC 
TTTACAGGAG TCTTCACCTT TGAAATGGTC ATAAAGATGA TAGACTTGGG CTTGCTGCTG 
CACCCTGGTG CCTACTTCCG GGACCTGTGG AACATTCTGG ACTTCATTGT TGTCAGTGGA 
GCCCTGGTGG CATTTGCGTT CTCAGGATCC AAAGGGAAAG ACATCAATAC CATCAAGTCT 
CTGAGAGTCC TGCGTGTCCT GAGGCCCCTC AAGACCATCA AGCGGCTGCC TAAGCTCAAG 
GCTGTCTTTG ACTGTGTGGT GAACTCCCTG AAGAACGTCT TGAACATCCT GATTGTCTAC 
ATGCTCTTCA TGTTCATATT TGCCGTCATT GCCGTCCAGC TCTTCAAAGG GAAGTTCTTT 
TACTGTACTG ATGAATCCAA GGAGCTGGAG AGGGACTGCC GGGGTCAGTA TTTGGATTAT 
GAGAAGGAAG AAGTAGAAGC CCAGCCAAGG CAGTGGAAGA AATATGACTT CCACTATGAC 
AATGTTCTCT GGGCCTTGTT GACGCTGTTC ACAGTGTCCA CGGGAGAGGG GTGGCCCATG 
GTGCTGAAAC ACTCTGTGGA TGCCACCTAT GAGGAACAGG GGCCCAGTCC CGGGTTCCGG 
ATGGAGCTCT CCATCTTCTA CGTGGTCTAC TTTGTGGTCT TCCCTTTTTT CTTTGTCAAC 
ATCTTTGTGG CCTTGATCAT TATCACCTTC CAGGAACAGG GAGATAAGGT GATGTCTGAA 
TGCAGCTTAG AAAAGAATGA GAGGGCTTGC ATTGATTTTG CCATCAGTGC CAAGCCCCTG 
ACACGGTACA TGCCTCAAAA CAAACAGTCG TTCCAGTATA AGACATGGAC ATTCGTGGTC 
TCTCCACCCT TTGAGTACTT CATCATGGCT ATGATAGCCC TCAACACAGT GGTGCTGATG 
ATGAAGTTCT ATGATGCACC TTATGAGTAC GAGCTGATGC TGAAATGCCT GAACATTGTC 
TTCACATCCA TGTTCTCGAT GGAGTGCATA CTGAAGATCA TCGCCTTTGG GGTATTGAAC 
TACTTCAGAG ATGCCTGGAA CGTCTTTGAC TTTGTCACGG TTTTGGGAAG TATTACTGAT 
ATTTTAGTAA CAGAGATAGC GAACAACTTC ATCAACCTAA GCTTCCTTCG CCTCTTCCGG 
GCGGCACGGC TGATCAAGCT GCTTCGCCAG GGCTACACCA TCCGCATCCT ATTGTGGACC 
TTCGTCCAGT CCTTTAAGGC GCTGCCCTAC GTGTGCCTCC TCATTGCCAT GCTGTTCTTC 
ATCTACGCCA TCATCGGCAT GCAGGTTTTT GGAAACATTG CCCTTGATGA TGACACCAGT 
ATCAACCGAC ACAACAACTT CCGGACATTT CTGCAAGCCT TAATGCTATT GTTCAGGAGT 
GCCACTGGGG AGGCCTGGCA TGAGATCATG CTGTCTTGTC TGGGCAACCG GGCCTGTGAC 
CCACATGCCA ACGCCAGTGA GTGCGGGAGC GACTTTGCCT ATTTTTATTT TGTCTCCTTC 
ATCTTCCTCT GTTCCTTTCT GATGTTGAAC CTCTTTGTTG CTGTAATCAT GGACAATTTT 
GAGTACCTCA CTCGGGACTC TTCCATCCTA GGGCCTCACC ACTTAGACGA ATTCATTCGA 
GTCTGGGCTG AATACGACCC AGCTGCGTGT GGGCGCATCA GTTACAATGA CATGTTTGAG 
ATGCTGAAAC ACATGTCCCC ACCTCTGGGG TTGGGGAAGA AATGCCCGGC TCGAGTTGCA 
TACAAGCGCC TGGTTCGCAT GAACATGCCC ATATCCAATG AGGACATGAC GGTGCACTTT 
ACGTCCACAC TGATGGCCCT CATCCGGACA GCACTGGAGA TCAAGCTTGC CCCAGCGGGG 
ACGAAGCAGC ACCAGTGTGA TGCTGAGCTG AGAAAAGAGA TCTCTTCTGT GTGGGCTAAT 
CTGCCCCAGA AGACTCTGGA CTTACTGGTA CCACCCCACA AACCTGACGA GATGACAGTG 
GGGAAGGTCT ATGCTGCTCT CATGATATTT GACTTCTACA AACAGAACAA AACCACCAGA 
GATCAGACTC ACCAAGCTCC CGGAGGCCTG TCCCAGATGG GTCCCGTTTC CCTGTTCCAC 
CCTCTGAAGG CCACCCTGGA ACAGACACAG CCCGCTGTGC TTCGAGGAGC TCGGGTTTTC 
CTTCGGCAAA AGAGTGCAAC TTCCCTCAGC AATGGGGGTG CCATACAAAC CCAGGAAAGT 
GGCATCAAGG AGTCGCTGTC CTGGGGCACG CAGAGGACCC AAGATGCACT TTATGAGGCC 
AGAGCACCTC TAGAACGTGG CCATTCTGCA GAGATCCCTG TGGGGCAGTC AGGAACACTG 
GCTGTGGATG TCCAGATGCA GAACATGACA CTGAGAGGAC CAGATGGGGA GCCCCAGCCT 
GGGCTGGAAA GCCAAGGCAG AGCTGCCTCT ATGCCACGCC TAGCGGCAGA AACACAGCCG 
GCCCCTAATG CCAGCCCCAT GAAGCGCTCC ATCTCCACAC TGGCTCCACG CCCACATGGG 
ACTCAGCTTT GCAGCACAGT TCTGGACCGG CCTCCTCCTA GCCAGGCATC ACATCACCAC 
CACCACCGCT GCCACCGGCG CAGAGACAAG AAGCAAAGGT CCCTGGAAAA GGGGCCCAGC 
CTGTCTGTTG ACCCAGAAGG TGCACCAAGC ACTGCTGCAG GACCTGGTCT GCCCCATGGA 
GAAGGATCCA CCGCCTGCCG GCGGGACCGT AAACAGGAGC GAGGCCGGTC CCAGGAGCGG 
AGGCAGCCCT CATCTTCCTC TTCAGAGAAG CAGCGCTTCT ATTCCTGTGA CCGCTTTGGG 
AGCCGGGAGC CCCCGCAACT GATGCCCTCA CTCAGTAGCC ACCCCACATC GCCAACAGCG 
GCGCTAGAGC CAGCACCCCA CCCACAGGGC AGTGGTTCCG TTAATGGGAG CCCCTTGATG 
TCAACATCCG GTGCTAGCAC CCCGGGCCGA GGTGGGCGGA GGCAGCTCCC CCAGACTCCT 
CTGACCCCAC GCCCCAGCAT CACCTACAAG ACCGCCAATT CCTCGCCTGT CCACTTTGCT 
GAGGGTCAGA GCGGCCTCCC AGCCTTCTCC CCTGGCCGTC TCAGCCGCGG CCTTTCTGAA 
CACAATGCCC TGCTCCAGAA AGAGCCCCTG AGCCAGCCTC TAGCTCCTGG CTCCCGAATT 
GGCTCTGACC CTTACCTAGG GCAGCGTCTG GACAGTGAGG CCTCCGCCCA CACCCTGCCT 
GAGGATACAC TCACCTTTGA AGAGGCAGTG GCCACCAACT CTGGCCGCTC CTCCAGGACT 
TCCTATGTGT CCTCCCTCAC TTCCCAATCC CACCCTCTCC GCCGTGTACC CAATGGCTAT 
CACTGCACTT TGGGACTCAG CACTGGCGTC CGGGCGCGGC ACAGCTACCA CCACCCCGAT 
CAGGACCACT GGTGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006497693.1
CDS	146..7183
Translation	MVRFGDELGGRYGGTGGGERARGGGAGGAGGPGQGGLPPGQRVLYKQSIAQRARTMALYNPIPVKQNCFTVNRSLFVFSEDNVVRKYAKRITEWPPFEYMILATIIANCIVLALEQHLPDGDKTPMSERLDDTEPYFIGIFCFEAGIKIIALGFVFHKGSYLRNGWNVMDFVVVLTGILATAGTDFDLRTLRAVRVLRPLKLVSGIPSLQVVLKSIMKAMVPLLQIGLLLFFAILMFAIIGLEFYMGKFHKACFPNSTDTEPVGDFPCGKDPPARQCDGDTECREYWPGPNFGITNFDNILFAILTVFQCITMEGWTDILYNTNDAAGNTWNWLYFIPLIIIGSFFMLNLVLGVLSGEFAKERERVENRRAFLKLRRQQQIERELNGYLEWIFKAEEVMLAEEDKNAEEKSPLDAVLKRAATKKSRNDLIHAEEGEDRFVDLCAVGSPFARASLKSGKTESSSYFRRKEKMFRFFIRRMVKAQSFYWVVLCVVALNTLCVAMVHYNQPQRLTTALYFAEFVFLGLFLTEMSLKMYGLGPRSYFRSSFNCFDFGVIVGSIFEVVWAAIKPGTSFGISVLRALRLLRIFKVTKYWNSLRNLVVSLLNSMKSIISLLFLLFLFIVVFALLGMQLFGGQFNFQDETPTTNFDTFPAAILTVFQILTGEDWNAVMYHGIESQGGVSKGMFSSFYFIVLTLFGNYTLLNVFLAIAVDNLANAQELTKDEEEMEEAANQKLALQKAKEVAEVSPMSAANISIAAFVKQTRGTVSRSSSVSSVNSPRQQNSAKARSVWEQRASQLRLQNLRASCEALYSEMDPEERLRYASTRHVRPDMKTHMDRPLVVEPGRDGLRGPVGSKSKPEGTEATESADLPRRHHRHRDRDKTSATAPAGGEQDRTESTETGAREERARPRRSHSKETPGADTQVRCERSRRHHRRGSPEEATEREPRRHRAHRHAQDSSKEGTAPVLVPKGERRARHRGPRTGPREAENNEEPTRRHRARHKVPPTLQPPEREAAEKESNAVEGDKETRNHQPKEPHCDLEAIAVTGVGPLHMLPSTCLQKVDEQPEDADNQRNVTRMGSQPSDPSTTVHVPVTLTGPPGETPVVPSGNMNLEGQAEGKKEAEADDVLRRGPRPIVPYSSMFCLSPTNLLRRFCHYIVTMRYFEMVILVVIALSSIALAAEDPVRTDSFRNNALKYMDYIFTGVFTFEMVIKMIDLGLLLHPGAYFRDLWNILDFIVVSGALVAFAFSGSKGKDINTIKSLRVLRVLRPLKTIKRLPKLKAVFDCVVNSLKNVLNILIVYMLFMFIFAVIAVQLFKGKFFYCTDESKELERDCRGQYLDYEKEEVEAQPRQWKKYDFHYDNVLWALLTLFTVSTGEGWPMVLKHSVDATYEEQGPSPGFRMELSIFYVVYFVVFPFFFVNIFVALIIITFQEQGDKVMSECSLEKNERACIDFAISAKPLTRYMPQNKQSFQYKTWTFVVSPPFEYFIMAMIALNTVVLMMKFYDAPYEYELMLKCLNIVFTSMFSMECILKIIAFGVLNYFRDAWNVFDFVTVLGSITDILVTEIANNFINLSFLRLFRAARLIKLLRQGYTIRILLWTFVQSFKALPYVCLLIAMLFFIYAIIGMQVFGNIALDDDTSINRHNNFRTFLQALMLLFRSATGEAWHEIMLSCLGNRACDPHANASECGSDFAYFYFVSFIFLCSFLMLNLFVAVIMDNFEYLTRDSSILGPHHLDEFIRVWAEYDPAACGRISYNDMFEMLKHMSPPLGLGKKCPARVAYKRLVRMNMPISNEDMTVHFTSTLMALIRTALEIKLAPAGTKQHQCDAELRKEISSVWANLPQKTLDLLVPPHKPDEMTVGKVYAALMIFDFYKQNKTTRDQTHQAPGGLSQMGPVSLFHPLKATLEQTQPAVLRGARVFLRQKSATSLSNGGAIQTQESGIKESLSWGTQRTQDALYEARAPLERGHSAEIPVGQSGTLAVDVQMQNMTLRGPDGEPQPGLESQGRAASMPRLAAETQPAPNASPMKRSISTLAPRPHGTQLCSTVLDRPPPSQASHHHHHRCHRRRDKKQRSLEKGPSLSVDPEGAPSTAAGPGLPHGEGSTACRRDRKQERGRSQERRQPSSSSSEKQRFYSCDRFGSREPPQLMPSLSSHPTSPTAALEPAPHPQGSGSVNGSPLMSTSGASTPGRGGRRQLPQTPLTPRPSITYKTANSSPVHFAEGQSGLPAFSPGRLSRGLSEHNALLQKEPLSQPLAPGSRIGSDPYLGQRLDSEASAHTLPEDTLTFEEAVATNSGRSSRTSYVSSLTSQSHPLRRVPNGYHCTLGLSTGVRARHSYHHPDQDHWC

Target ORF information:

RefSeq Version	XM_006497630.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus calcium channel, voltage-dependent, N type, alpha 1B subunit (Cacna1b), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006497630.3

ORF Insert Sequence:

ATGGTCCGCT TCGGGGACGA GCTAGGCGGC CGCTATGGGG GCACCGGCGG CGGGGAGCGG 
GCTCGGGGTG GCGGGGCCGG CGGGGCGGGT GGCCCGGGCC AGGGGGGTCT GCCGCCGGGC 
CAGCGGGTCC TGTACAAGCA GTCCATTGCG CAGCGCGCAC GGACTATGGC CCTGTACAAC 
CCCATCCCAG TCAAGCAGAA CTGCTTCACC GTCAACCGCT CGCTCTTCGT CTTCAGCGAG 
GACAACGTCG TCCGCAAATA CGCTAAGCGC ATCACCGAAT GGCCGCCCTT CGAATACATG 
ATCCTGGCCA CCATCATCGC CAACTGCATT GTTCTGGCCC TGGAGCAGCA CCTCCCTGAT 
GGGGACAAGA CTCCCATGTC TGAGCGACTA GATGACACGG AGCCTTACTT CATCGGGATC 
TTTTGCTTCG AGGCGGGCAT CAAGATCATA GCCCTGGGCT TTGTTTTCCA CAAGGGCTCC 
TACCTTCGGA ACGGCTGGAA TGTCATGGAC TTCGTGGTGG TCCTCACGGG GATTCTCGCC 
ACAGCTGGAA CTGACTTTGA CCTGCGCACA CTGAGGGCTG TGCGTGTGCT TAGGCCCCTG 
AAGCTGGTGT CTGGAATTCC AAGCTTGCAG GTGGTGCTTA AGTCCATCAT GAAGGCCATG 
GTCCCGCTGC TGCAGATTGG GCTGCTGCTC TTCTTTGCCA TCCTCATGTT TGCCATCATC 
GGCCTCGAAT TCTATATGGG CAAATTCCAT AAGGCCTGTT TCCCCAACAG CACAGATACA 
GAGCCTGTGG GTGACTTTCC CTGTGGCAAA GATCCCCCTG CTCGTCAGTG TGATGGTGAC 
ACCGAATGCC GGGAGTACTG GCCAGGACCC AACTTTGGCA TCACCAATTT TGACAACATC 
CTGTTTGCCA TCTTGACAGT GTTCCAGTGT ATCACCATGG AGGGCTGGAC TGACATCCTC 
TACAATACAA ATGATGCGGC TGGCAACACA TGGAACTGGT TGTACTTCAT CCCCCTCATC 
ATCATTGGCT CCTTCTTCAT GCTCAACCTG GTGCTGGGTG TGCTTTCCGG AGAGTTTGCC 
AAGGAGCGGG AGCGAGTCGA GAACCGCCGC GCCTTCCTGA AGCTCCGCAG GCAGCAGCAG 
ATTGAGCGAG AGCTGAATGG GTACTTGGAG TGGATCTTCA AGGCAGAGGA AGTCATGTTG 
GCAGAGGAGG ACAAGAATGC AGAAGAGAAA TCCCCTTTGG ATGCAGTGTT GAAGAGAGCT 
GCCACCAAGA AGAGCCGAAA TGACCTCATC CATGCAGAAG AGGGGGAGGA CCGGTTTGTA 
GACCTCTGTG CAGTTGGGTC TCCATTTGCT CGTGCCAGCC TCAAGAGTGG GAAGACGGAG 
AGCTCATCGT ACTTCCGGAG AAAGGAGAAG ATGTTCCGGT TCTTTATCCG GCGTATGGTG 
AAAGCACAGA GCTTCTACTG GGTGGTACTG TGTGTGGTGG CCCTGAACAC ACTGTGTGTG 
GCCATGGTGC ACTATAATCA GCCTCAGCGG CTTACCACTG CACTGTACTT TGCAGAGTTT 
GTTTTCCTGG GTCTCTTCCT CACAGAGATG TCCCTGAAGA TGTATGGCCT AGGGCCCAGA 
AGTTACTTCA GGTCTTCCTT CAACTGCTTT GACTTTGGGG TGATTGTGGG GAGTATCTTT 
GAAGTAGTCT GGGCTGCCAT CAAGCCAGGA ACCTCCTTTG GAATCAGTGT GCTGCGGGCT 
CTGCGACTGC TGAGGATATT CAAAGTTACC AAGTATTGGA ACTCTCTGAG GAACCTGGTG 
GTTTCCCTCC TCAATTCCAT GAAGTCCATC ATCAGCCTTC TCTTCCTGCT TTTCCTCTTC 
ATCGTGGTCT TCGCTCTGTT GGGGATGCAG CTGTTTGGGG GACAGTTCAA CTTTCAAGAT 
GAGACTCCAA CCACCAATTT TGATACCTTC CCAGCTGCCA TCCTCACTGT CTTTCAGATC 
CTGACAGGAG AGGATTGGAA TGCCGTAATG TATCATGGGA TTGAGTCGCA AGGTGGAGTC 
AGCAAAGGCA TGTTTTCTTC CTTTTACTTC ATCGTCCTGA CACTGTTTGG AAACTACACC 
CTGCTGAATG TTTTTCTGGC CATTGCTGTG GACAACCTTG CCAATGCCCA GGAGTTGACC 
AAGGATGAAG AGGAGATGGA AGAAGCAGCC AATCAGAAAC TTGCTCTTCA GAAGGCCAAA 
GAAGTAGCTG AAGTCAGCCC CATGTCTGCT GCCAATATCT CCATCGCTGC TTTTGTAAAG 
CAAACTCGAG GTACTGTATC TCGCAGCTCA TCTGTCTCCA GCGTAAACTC ACCCAGGCAG 
CAGAACTCGG CCAAGGCGCG CTCAGTATGG GAGCAGCGGG CCAGTCAGCT AAGGCTCCAG 
AATCTGCGTG CCAGCTGTGA GGCATTGTAC AGTGAGATGG ACCCTGAGGA GCGCCTGCGT 
TATGCCAGCA CGCGCCATGT GAGGCCAGAC ATGAAGACAC ACATGGACCG ACCCCTAGTG 
GTGGAGCCTG GTCGAGATGG CTTGCGGGGA CCCGTTGGGA GCAAGTCAAA GCCTGAAGGC 
ACGGAGGCCA CAGAAAGCGC GGACCTACCT CGCAGGCACC ACCGGCACCG TGATAGGGAC 
AAGACCTCAG CCACAGCACC TGCTGGAGGC GAACAGGACA GGACAGAAAG CACCGAGACC 
GGGGCCCGGG AGGAACGTGC GCGCCCTCGT CGAAGTCACA GCAAGGAGAC TCCAGGGGCT 
GACACGCAAG TGCGCTGTGA GCGCAGTAGA CGTCACCACC GGCGCGGCTC CCCGGAGGAG 
GCCACTGAAC GGGAGCCTCG GCGCCACCGT GCCCACCGGC ATGCACAGGA CTCAAGCAAG 
GAGGGCACGG CGCCGGTGCT TGTACCCAAG GGTGAGCGAC GAGCAAGACA CCGAGGCCCA 
CGCACGGGTC CACGTGAGGC AGAGAACAAC GAGGAGCCCA CACGCAGGCA CCGTGCAAGG 
CATAAGGTGC CACCCACACT GCAGCCCCCA GAGAGGGAGG CTGCAGAGAA GGAGAGCAAC 
GCGGTGGAAG GGGATAAGGA AACCCGAAAT CACCAGCCCA AGGAACCTCA CTGTGACCTG 
GAGGCCATTG CAGTTACAGG TGTGGGCCCT CTGCACATGC TGCCCAGCAC CTGTCTCCAG 
AAAGTGGACG AGCAACCAGA GGATGCAGAC AACCAGCGTA ATGTCACCCG GATGGGCAGT 
CAGCCCTCAG ATCCCAGCAC CACTGTGCAT GTCCCAGTGA CACTGACAGG CCCTCCTGGG 
GAGACCCCTG TAGTTCCCAG TGGTAACATG AACCTGGAAG GCCAAGCAGA GGGCAAGAAG 
GAGGCAGAGG CGGATGATGT GCTGAGAAGA GGCCCCAGGC CCATCGTTCC CTACAGCTCC 
ATGTTTTGTC TCAGCCCCAC CAACCTGCTT CGTCGCTTCT GCCATTACAT TGTGACCATG 
CGGTACTTTG AGATGGTAAT TCTTGTGGTC ATTGCCTTGA GCAGCATTGC CCTGGCTGCA 
GAGGATCCTG TGCGGACAGA TTCATTCAGG AACAACGCTT TAAAGTACAT GGATTACATC 
TTTACAGGAG TCTTCACCTT TGAAATGGTC ATAAAGATGA TAGACTTGGG CTTGCTGCTG 
CACCCTGGTG CCTACTTCCG GGACCTGTGG AACATTCTGG ACTTCATTGT TGTCAGTGGA 
GCCCTGGTGG CATTTGCGTT CTCAGGATCC AAAGGGAAAG ACATCAATAC CATCAAGTCT 
CTGAGAGTCC TGCGTGTCCT GAGGCCCCTC AAGACCATCA AGCGGCTGCC TAAGCTCAAG 
GCTGTCTTTG ACTGTGTGGT GAACTCCCTG AAGAACGTCT TGAACATCCT GATTGTCTAC 
ATGCTCTTCA TGTTCATATT TGCCGTCATT GCCGTCCAGC TCTTCAAAGG GAAGTTCTTT 
TACTGTACTG ATGAATCCAA GGAGCTGGAG AGGGACTGCC GGGGTCAGTA TTTGGATTAT 
GAGAAGGAAG AAGTAGAAGC CCAGCCAAGG CAGTGGAAGA AATATGACTT CCACTATGAC 
AATGTTCTCT GGGCCTTGTT GACGCTGTTC ACAGTGTCCA CGGGAGAGGG GTGGCCCATG 
GTGCTGAAAC ACTCTGTGGA TGCCACCTAT GAGGAACAGG GGCCCAGTCC CGGGTTCCGG 
ATGGAGCTCT CCATCTTCTA CGTGGTCTAC TTTGTGGTCT TCCCTTTTTT CTTTGTCAAC 
ATCTTTGTGG CCTTGATCAT TATCACCTTC CAGGAACAGG GAGATAAGGT GATGTCTGAA 
TGCAGCTTAG AAAAGAATGA GAGGGCTTGC ATTGATTTTG CCATCAGTGC CAAGCCCCTG 
ACACGGTACA TGCCTCAAAA CAAACAGTCG TTCCAGTATA AGACATGGAC ATTCGTGGTC 
TCTCCACCCT TTGAGTACTT CATCATGGCT ATGATAGCCC TCAACACAGT GGTGCTGATG 
ATGAAGTTCT ATGATGCACC TTATGAGTAC GAGCTGATGC TGAAATGCCT GAACATTGTC 
TTCACATCCA TGTTCTCGAT GGAGTGCATA CTGAAGATCA TCGCCTTTGG GGTATTGAAC 
TACTTCAGAG ATGCCTGGAA CGTCTTTGAC TTTGTCACGG TTTTGGGAAG TATTACTGAT 
ATTTTAGTAA CAGAGATAGC GAACAACTTC ATCAACCTAA GCTTCCTTCG CCTCTTCCGG 
GCGGCACGGC TGATCAAGCT GCTTCGCCAG GGCTACACCA TCCGCATCCT ATTGTGGACC 
TTCGTCCAGT CCTTTAAGGC GCTGCCCTAC GTGTGCCTCC TCATTGCCAT GCTGTTCTTC 
ATCTACGCCA TCATCGGCAT GCAGGTTTTT GGAAACATTG CCCTTGATGA TGACACCAGT 
ATCAACCGAC ACAACAACTT CCGGACATTT CTGCAAGCCT TAATGCTATT GTTCAGGAGT 
GCCACTGGGG AGGCCTGGCA TGAGATCATG CTGTCTTGTC TGGGCAACCG GGCCTGTGAC 
CCACATGCCA ACGCCAGTGA GTGCGGGAGC GACTTTGCCT ATTTTTATTT TGTCTCCTTC 
ATCTTCCTCT GTTCCTTTCT GATGTTGAAC CTCTTTGTTG CTGTAATCAT GGACAATTTT 
GAGTACCTCA CTCGGGACTC TTCCATCCTA GGGCCTCACC ACTTAGACGA ATTCATTCGA 
GTCTGGGCTG AATACGACCC AGCTGCGTGT GGGCGCATCA GTTACAATGA CATGTTTGAG 
ATGCTGAAAC ACATGTCCCC ACCTCTGGGG TTGGGGAAGA AATGCCCGGC TCGAGTTGCA 
TACAAGCGCC TGGTTCGCAT GAACATGCCC ATATCCAATG AGGACATGAC GGTGCACTTT 
ACGTCCACAC TGATGGCCCT CATCCGGACA GCACTGGAGA TCAAGCTTGC CCCAGCGGGG 
ACGAAGCAGC ACCAGTGTGA TGCTGAGCTG AGAAAAGAGA TCTCTTCTGT GTGGGCTAAT 
CTGCCCCAGA AGACTCTGGA CTTACTGGTA CCACCCCACA AACCTGACGA GATGACAGTG 
GGGAAGGTCT ATGCTGCTCT CATGATATTT GACTTCTACA AACAGAACAA AACCACCAGA 
GATCAGACTC ACCAAGCTCC CGGAGGCCTG TCCCAGATGG GTCCCGTTTC CCTGTTCCAC 
CCTCTGAAGG CCACCCTGGA ACAGACACAG CCCGCTGTGC TTCGAGGAGC TCGGGTTTTC 
CTTCGGCAAA AGAGTGCAAC TTCCCTCAGC AATGGGGGTG CCATACAAAC CCAGGAAAGT 
GGCATCAAGG AGTCGCTGTC CTGGGGCACG CAGAGGACCC AAGATGCACT TTATGAGGCC 
AGAGCACCTC TAGAACGTGG CCATTCTGCA GAGATCCCTG TGGGGCAGTC AGGAACACTG 
GCTGTGGATG TCCAGATGCA GAACATGACA CTGAGAGGAC CAGATGGGGA GCCCCAGCCT 
GGGCTGGAAA GCCAAGGCAG AGCTGCCTCT ATGCCACGCC TAGCGGCAGA AACACAGCCG 
GCCCCTAATG CCAGCCCCAT GAAGCGCTCC ATCTCCACAC TGGCTCCACG CCCACATGGG 
ACTCAGCTTT GCAGCACAGT TCTGGACCGG CCTCCTCCTA GCCAGGCATC ACATCACCAC 
CACCACCGCT GCCACCGGCG CAGAGACAAG AAGCAAAGGT CCCTGGAAAA GGGGCCCAGC 
CTGTCTGTTG ACCCAGAAGG TGCACCAAGC ACTGCTGCAG GACCTGGTCT GCCCCATGGA 
GAAGGATCCA CCGCCTGCCG GCGGGACCGT AAACAGGAGC GAGGCCGGTC CCAGGAGCGG 
AGGCAGCCCT CATCTTCCTC TTCAGAGAAG CAGCGCTTCT ATTCCTGTGA CCGCTTTGGG 
AGCCGGGAGC CCCCGCAACT GATGCCCTCA CTCAGTAGCC ACCCCACATC GCCAACAGCG 
GCGCTAGAGC CAGCACCCCA CCCACAGGGC AGTGGTTCCG TTAATGGGAG CCCCTTGATG 
TCAACATCCG GTGCTAGCAC CCCGGGCCGA GGTGGGCGGA GGCAGCTCCC CCAGACTCCT 
CTGACCCCAC GCCCCAGCAT CACCTACAAG ACCGCCAATT CCTCGCCTGT CCACTTTGCT 
GAGGGTCAGA GCGGCCTCCC AGCCTTCTCC CCTGGCCGTC TCAGCCGCGG CCTTTCTGAA 
CACAATGCCC TGCTCCAGAA AGAGCCCCTG AGCCAGCCTC TAGCTCCTGG CTCCCGAATT 
GGCTCTGACC CTTACCTAGG GCAGCGTCTG GACAGTGAGG CCTCCGCCCA CACCCTGCCT 
GAGGATACAC TCACCTTTGA AGAGGCAGTG GCCACCAACT CTGGCCGCTC CTCCAGGACT 
TCCTATGTGT CCTCCCTCAC TTCCCAATCC CACCCTCTCC GCCGTGTACC CAATGGCTAT 
CACTGCACTT TGGGACTCAG CACTGGCGTC CGGGCGCGGC ACAGCTACCA CCACCCCGAT 
CAGGACCACT GGTGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cacna1b ( XM_006497631.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006497694.1 Mus musculus calcium channel, voltage-dependent, N type, alpha 1B subunit (Cacna1b), transcript variant X8, mRNA.

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CloneID	OMu29262
Clone ID Related Accession (Same CDS sequence)	XM_006497631.3
Accession Version	XM_006497631.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6993bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	voltage-dependent N-type calcium channel subunit alpha-1B isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006497631.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTCCGCT TCGGGGACGA GCTAGGCGGC CGCTATGGGG GCACCGGCGG CGGGGAGCGG 
GCTCGGGGTG GCGGGGCCGG CGGGGCGGGT GGCCCGGGCC AGGGGGGTCT GCCGCCGGGC 
CAGCGGGTCC TGTACAAGCA GTCCATTGCG CAGCGCGCAC GGACTATGGC CCTGTACAAC 
CCCATCCCAG TCAAGCAGAA CTGCTTCACC GTCAACCGCT CGCTCTTCGT CTTCAGCGAG 
GACAACGTCG TCCGCAAATA CGCTAAGCGC ATCACCGAAT GGCCGCCCTT CGAATACATG 
ATCCTGGCCA CCATCATCGC CAACTGCATT GTTCTGGCCC TGGAGCAGCA CCTCCCTGAT 
GGGGACAAGA CTCCCATGTC TGAGCGACTA GATGACACGG AGCCTTACTT CATCGGGATC 
TTTTGCTTCG AGGCGGGCAT CAAGATCATA GCCCTGGGCT TTGTTTTCCA CAAGGGCTCC 
TACCTTCGGA ACGGCTGGAA TGTCATGGAC TTCGTGGTGG TCCTCACGGG GATTCTCGCC 
ACAGCTGGAA CTGACTTTGA CCTGCGCACA CTGAGGGCTG TGCGTGTGCT TAGGCCCCTG 
AAGCTGGTGT CTGGAATTCC AAGCTTGCAG GTGGTGCTTA AGTCCATCAT GAAGGCCATG 
GTCCCGCTGC TGCAGATTGG GCTGCTGCTC TTCTTTGCCA TCCTCATGTT TGCCATCATC 
GGCCTCGAAT TCTATATGGG CAAATTCCAT AAGGCCTGTT TCCCCAACAG CACAGATACA 
GAGCCTGTGG GTGACTTTCC CTGTGGCAAA GATCCCCCTG CTCGTCAGTG TGATGGTGAC 
ACCGAATGCC GGGAGTACTG GCCAGGACCC AACTTTGGCA TCACCAATTT TGACAACATC 
CTGTTTGCCA TCTTGACAGT GTTCCAGTGT ATCACCATGG AGGGCTGGAC TGACATCCTC 
TACAATACAA ATGATGCGGC TGGCAACACA TGGAACTGGT TGTACTTCAT CCCCCTCATC 
ATCATTGGCT CCTTCTTCAT GCTCAACCTG GTGCTGGGTG TGCTTTCCGG AGAGTTTGCC 
AAGGAGCGGG AGCGAGTCGA GAACCGCCGC GCCTTCCTGA AGCTCCGCAG GCAGCAGCAG 
ATTGAGCGAG AGCTGAATGG GTACTTGGAG TGGATCTTCA AGGCAGAGGA AGTCATGTTG 
GCAGAGGAGG ACAAGAATGC AGAAGAGAAA TCCCCTTTGG ATGCAGTGTT GAAGAGAGCT 
GCCACCAAGA AGAGCCGAAA TGACCTCATC CATGCAGAAG AGGGGGAGGA CCGGTTTGTA 
GACCTCTGTG CAGTTGGGTC TCCATTTGCT CGTGCCAGCC TCAAGAGTGG GAAGACGGAG 
AGCTCATCGT ACTTCCGGAG AAAGGAGAAG ATGTTCCGGT TCTTTATCCG GCGTATGGTG 
AAAGCACAGA GCTTCTACTG GGTGGTACTG TGTGTGGTGG CCCTGAACAC ACTGTGTGTG 
GCCATGGTGC ACTATAATCA GCCTCAGCGG CTTACCACTG CACTGTACTT TGCAGAGTTT 
GTTTTCCTGG GTCTCTTCCT CACAGAGATG TCCCTGAAGA TGTATGGCCT AGGGCCCAGA 
AGTTACTTCA GGTCTTCCTT CAACTGCTTT GACTTTGGGG TGATTGTGGG GAGTATCTTT 
GAAGTAGTCT GGGCTGCCAT CAAGCCAGGA ACCTCCTTTG GAATCAGTGT GCTGCGGGCT 
CTGCGACTGC TGAGGATATT CAAAGTTACC AAGTATTGGA ACTCTCTGAG GAACCTGGTG 
GTTTCCCTCC TCAATTCCAT GAAGTCCATC ATCAGCCTTC TCTTCCTGCT TTTCCTCTTC 
ATCGTGGTCT TCGCTCTGTT GGGGATGCAG CTGTTTGGGG GACAGTTCAA CTTTCAAGAT 
GAGACTCCAA CCACCAATTT TGATACCTTC CCAGCTGCCA TCCTCACTGT CTTTCAGATC 
CTGACAGGAG AGGATTGGAA TGCCGTAATG TATCATGGGA TTGAGTCGCA AGGTGGAGTC 
AGCAAAGGCA TGTTTTCTTC CTTTTACTTC ATCGTCCTGA CACTGTTTGG AAACTACACC 
CTGCTGAATG TTTTTCTGGC CATTGCTGTG GACAACCTTG CCAATGCCCA GGAGTTGACC 
AAGGATGAAG AGGAGATGGA AGAAGCAGCC AATCAGAAAC TTGCTCTTCA GAAGGCCAAA 
GAAGTAGCTG AAGTCAGCCC CATGTCTGCT GCCAATATCT CCATCGCTGC CAGGCAGCAG 
AACTCGGCCA AGGCGCGCTC AGTATGGGAG CAGCGGGCCA GTCAGCTAAG GCTCCAGAAT 
CTGCGTGCCA GCTGTGAGGC ATTGTACAGT GAGATGGACC CTGAGGAGCG CCTGCGTTAT 
GCCAGCACGC GCCATGTGAG GCCAGACATG AAGACACACA TGGACCGACC CCTAGTGGTG 
GAGCCTGGTC GAGATGGCTT GCGGGGACCC GTTGGGAGCA AGTCAAAGCC TGAAGGCACG 
GAGGCCACAG AAAGCGCGGA CCTACCTCGC AGGCACCACC GGCACCGTGA TAGGGACAAG 
ACCTCAGCCA CAGCACCTGC TGGAGGCGAA CAGGACAGGA CAGAAAGCAC CGAGACCGGG 
GCCCGGGAGG AACGTGCGCG CCCTCGTCGA AGTCACAGCA AGGAGACTCC AGGGGCTGAC 
ACGCAAGTGC GCTGTGAGCG CAGTAGACGT CACCACCGGC GCGGCTCCCC GGAGGAGGCC 
ACTGAACGGG AGCCTCGGCG CCACCGTGCC CACCGGCATG CACAGGACTC AAGCAAGGAG 
GGCACGGCGC CGGTGCTTGT ACCCAAGGGT GAGCGACGAG CAAGACACCG AGGCCCACGC 
ACGGGTCCAC GTGAGGCAGA GAACAACGAG GAGCCCACAC GCAGGCACCG TGCAAGGCAT 
AAGGTGCCAC CCACACTGCA GCCCCCAGAG AGGGAGGCTG CAGAGAAGGA GAGCAACGCG 
GTGGAAGGGG ATAAGGAAAC CCGAAATCAC CAGCCCAAGG AACCTCACTG TGACCTGGAG 
GCCATTGCAG TTACAGGTGT GGGCCCTCTG CACATGCTGC CCAGCACCTG TCTCCAGAAA 
GTGGACGAGC AACCAGAGGA TGCAGACAAC CAGCGTAATG TCACCCGGAT GGGCAGTCAG 
CCCTCAGATC CCAGCACCAC TGTGCATGTC CCAGTGACAC TGACAGGCCC TCCTGGGGAG 
ACCCCTGTAG TTCCCAGTGG TAACATGAAC CTGGAAGGCC AAGCAGAGGG CAAGAAGGAG 
GCAGAGGCGG ATGATGTGCT GAGAAGAGGC CCCAGGCCCA TCGTTCCCTA CAGCTCCATG 
TTTTGTCTCA GCCCCACCAA CCTGCTTCGT CGCTTCTGCC ATTACATTGT GACCATGCGG 
TACTTTGAGA TGGTAATTCT TGTGGTCATT GCCTTGAGCA GCATTGCCCT GGCTGCAGAG 
GATCCTGTGC GGACAGATTC ATTCAGGAAC AACGCTTTAA AGTACATGGA TTACATCTTT 
ACAGGAGTCT TCACCTTTGA AATGGTCATA AAGATGATAG ACTTGGGCTT GCTGCTGCAC 
CCTGGTGCCT ACTTCCGGGA CCTGTGGAAC ATTCTGGACT TCATTGTTGT CAGTGGAGCC 
CTGGTGGCAT TTGCGTTCTC GAGCTTCATG GGAGGATCCA AAGGGAAAGA CATCAATACC 
ATCAAGTCTC TGAGAGTCCT GCGTGTCCTG AGGCCCCTCA AGACCATCAA GCGGCTGCCT 
AAGCTCAAGG CTGTCTTTGA CTGTGTGGTG AACTCCCTGA AGAACGTCTT GAACATCCTG 
ATTGTCTACA TGCTCTTCAT GTTCATATTT GCCGTCATTG CCGTCCAGCT CTTCAAAGGG 
AAGTTCTTTT ACTGTACTGA TGAATCCAAG GAGCTGGAGA GGGACTGCCG GGGTCAGTAT 
TTGGATTATG AGAAGGAAGA AGTAGAAGCC CAGCCAAGGC AGTGGAAGAA ATATGACTTC 
CACTATGACA ATGTTCTCTG GGCCTTGTTG ACGCTGTTCA CAGTGTCCAC GGGAGAGGGG 
TGGCCCATGG TGCTGAAACA CTCTGTGGAT GCCACCTATG AGGAACAGGG GCCCAGTCCC 
GGGTTCCGGA TGGAGCTCTC CATCTTCTAC GTGGTCTACT TTGTGGTCTT CCCTTTTTTC 
TTTGTCAACA TCTTTGTGGC CTTGATCATT ATCACCTTCC AGGAACAGGG AGATAAGGTG 
ATGTCTGAAT GCAGCTTAGA AAAGAATGAG AGGGCTTGCA TTGATTTTGC CATCAGTGCC 
AAGCCCCTGA CACGGTACAT GCCTCAAAAC AAACAGTCGT TCCAGTATAA GACATGGACA 
TTCGTGGTCT CTCCACCCTT TGAGTACTTC ATCATGGCTA TGATAGCCCT CAACACAGTG 
GTGCTGATGA TGAAGTTCTA TGATGCACCT TATGAGTACG AGCTGATGCT GAAATGCCTG 
AACATTGTCT TCACATCCAT GTTCTCGATG GAGTGCATAC TGAAGATCAT CGCCTTTGGG 
GTATTGAACT ACTTCAGAGA TGCCTGGAAC GTCTTTGACT TTGTCACGGT TTTGGGAAGT 
ATTACTGATA TTTTAGTAAC AGAGATAGCG GAAACGAACA ACTTCATCAA CCTAAGCTTC 
CTTCGCCTCT TCCGGGCGGC ACGGCTGATC AAGCTGCTTC GCCAGGGCTA CACCATCCGC 
ATCCTATTGT GGACCTTCGT CCAGTCCTTT AAGGCGCTGC CCTACGTGTG CCTCCTCATT 
GCCATGCTGT TCTTCATCTA CGCCATCATC GGCATGCAGG TTTTTGGAAA CATTGCCCTT 
GATGATGACA CCAGTATCAA CCGACACAAC AACTTCCGGA CATTTCTGCA AGCCTTAATG 
CTATTGTTCA GGAGTGCCAC TGGGGAGGCC TGGCATGAGA TCATGCTGTC TTGTCTGGGC 
AACCGGGCCT GTGACCCACA TGCCAACGCC AGTGAGTGCG GGAGCGACTT TGCCTATTTT 
TATTTTGTCT CCTTCATCTT CCTCTGTTCC TTTCTGATGT TGAACCTCTT TGTTGCTGTA 
ATCATGGACA ATTTTGAGTA CCTCACTCGG GACTCTTCCA TCCTAGGGCC TCACCACTTA 
GACGAATTCA TTCGAGTCTG GGCTGAATAC GACCCAGCTG CGTGTGGGCG CATCAGTTAC 
AATGACATGT TTGAGATGCT GAAACACATG TCCCCACCTC TGGGGTTGGG GAAGAAATGC 
CCGGCTCGAG TTGCATACAA GCGCCTGGTT CGCATGAACA TGCCCATATC CAATGAGGAC 
ATGACGGTGC ACTTTACGTC CACACTGATG GCCCTCATCC GGACAGCACT GGAGATCAAG 
CTTGCCCCAG CGGGGACGAA GCAGCACCAG TGTGATGCTG AGCTGAGAAA AGAGATCTCT 
TCTGTGTGGG CTAATCTGCC CCAGAAGACT CTGGACTTAC TGGTACCACC CCACAAACCT 
GACGAGATGA CAGTGGGGAA GGTCTATGCT GCTCTCATGA TATTTGACTT CTACAAACAG 
AACAAAACCA CCAGAGATCA GACTCACCAA GCTCCCGGAG GCCTGTCCCA GATGGGTCCC 
GTTTCCCTGT TCCACCCTCT GAAGGCCACC CTGGAACAGA CACAGCCCGC TGTGCTTCGA 
GGAGCTCGGG TTTTCCTTCG GCAAAAGAGT GCAACTTCCC TCAGCAATGG GGGTGCCATA 
CAAACCCAGG AAAGTGGCAT CAAGGAGTCG CTGTCCTGGG GCACGCAGAG GACCCAAGAT 
GCACTTTATG AGGCCAGAGC ACCTCTAGAA CGTGGCCATT CTGCAGAGAT CCCTGTGGGG 
CAGTCAGGAA CACTGGCTGT GGATGTCCAG ATGCAGAACA TGACACTGAG AGGACCAGAT 
GGGGAGCCCC AGCCTGGGCT GGAAAGCCAA GGCAGAGCTG CCTCTATGCC ACGCCTAGCG 
GCAGAAACAC AGCCGGCCCC TAATGCCAGC CCCATGAAGC GCTCCATCTC CACACTGGCT 
CCACGCCCAC ATGGGACTCA GCTTTGCAGC ACAGTTCTGG ACCGGCCTCC TCCTAGCCAG 
GCATCACATC ACCACCACCA CCGCTGCCAC CGGCGCAGAG ACAAGAAGCA AAGGTCCCTG 
GAAAAGGGGC CCAGCCTGTC TGTTGACCCA GAAGGTGCAC CAAGCACTGC TGCAGGACCT 
GGTCTGCCCC ATGGAGAAGG ATCCACCGCC TGCCGGCGGG ACCGTAAACA GGAGCGAGGC 
CGGTCCCAGG AGCGGAGGCA GCCCTCATCT TCCTCTTCAG AGAAGCAGCG CTTCTATTCC 
TGTGACCGCT TTGGGAGCCG GGAGCCCCCG CAACTGATGC CCTCACTCAG TAGCCACCCC 
ACATCGCCAA CAGCGGCGCT AGAGCCAGCA CCCCACCCAC AGGGCAGTGG TTCCGTTAAT 
GGGAGCCCCT TGATGTCAAC ATCCGGTGCT AGCACCCCGG GCCGAGGTGG GCGGAGGCAG 
CTCCCCCAGA CTCCTCTGAC CCCACGCCCC AGCATCACCT ACAAGACCGC CAATTCCTCG 
CCTGTCCACT TTGCTGAGGG TCAGAGCGGC CTCCCAGCCT TCTCCCCTGG CCGTCTCAGC 
CGCGGCCTTT CTGAACACAA TGCCCTGCTC CAGAAAGAGC CCCTGAGCCA GCCTCTAGCT 
CCTGGCTCCC GAATTGGCTC TGACCCTTAC CTAGGGCAGC GTCTGGACAG TGAGGCCTCC 
GCCCACACCC TGCCTGAGGA TACACTCACC TTTGAAGAGG CAGTGGCCAC CAACTCTGGC 
CGCTCCTCCA GGACTTCCTA TGTGTCCTCC CTCACTTCCC AATCCCACCC TCTCCGCCGT 
GTACCCAATG GCTATCACTG CACTTTGGGA CTCAGCACTG GCGTCCGGGC GCGGCACAGC 
TACCACCACC CCGATCAGGA CCACTGGTGC TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006497694.1
CDS	146..7138
Translation	MVRFGDELGGRYGGTGGGERARGGGAGGAGGPGQGGLPPGQRVLYKQSIAQRARTMALYNPIPVKQNCFTVNRSLFVFSEDNVVRKYAKRITEWPPFEYMILATIIANCIVLALEQHLPDGDKTPMSERLDDTEPYFIGIFCFEAGIKIIALGFVFHKGSYLRNGWNVMDFVVVLTGILATAGTDFDLRTLRAVRVLRPLKLVSGIPSLQVVLKSIMKAMVPLLQIGLLLFFAILMFAIIGLEFYMGKFHKACFPNSTDTEPVGDFPCGKDPPARQCDGDTECREYWPGPNFGITNFDNILFAILTVFQCITMEGWTDILYNTNDAAGNTWNWLYFIPLIIIGSFFMLNLVLGVLSGEFAKERERVENRRAFLKLRRQQQIERELNGYLEWIFKAEEVMLAEEDKNAEEKSPLDAVLKRAATKKSRNDLIHAEEGEDRFVDLCAVGSPFARASLKSGKTESSSYFRRKEKMFRFFIRRMVKAQSFYWVVLCVVALNTLCVAMVHYNQPQRLTTALYFAEFVFLGLFLTEMSLKMYGLGPRSYFRSSFNCFDFGVIVGSIFEVVWAAIKPGTSFGISVLRALRLLRIFKVTKYWNSLRNLVVSLLNSMKSIISLLFLLFLFIVVFALLGMQLFGGQFNFQDETPTTNFDTFPAAILTVFQILTGEDWNAVMYHGIESQGGVSKGMFSSFYFIVLTLFGNYTLLNVFLAIAVDNLANAQELTKDEEEMEEAANQKLALQKAKEVAEVSPMSAANISIAARQQNSAKARSVWEQRASQLRLQNLRASCEALYSEMDPEERLRYASTRHVRPDMKTHMDRPLVVEPGRDGLRGPVGSKSKPEGTEATESADLPRRHHRHRDRDKTSATAPAGGEQDRTESTETGAREERARPRRSHSKETPGADTQVRCERSRRHHRRGSPEEATEREPRRHRAHRHAQDSSKEGTAPVLVPKGERRARHRGPRTGPREAENNEEPTRRHRARHKVPPTLQPPEREAAEKESNAVEGDKETRNHQPKEPHCDLEAIAVTGVGPLHMLPSTCLQKVDEQPEDADNQRNVTRMGSQPSDPSTTVHVPVTLTGPPGETPVVPSGNMNLEGQAEGKKEAEADDVLRRGPRPIVPYSSMFCLSPTNLLRRFCHYIVTMRYFEMVILVVIALSSIALAAEDPVRTDSFRNNALKYMDYIFTGVFTFEMVIKMIDLGLLLHPGAYFRDLWNILDFIVVSGALVAFAFSSFMGGSKGKDINTIKSLRVLRVLRPLKTIKRLPKLKAVFDCVVNSLKNVLNILIVYMLFMFIFAVIAVQLFKGKFFYCTDESKELERDCRGQYLDYEKEEVEAQPRQWKKYDFHYDNVLWALLTLFTVSTGEGWPMVLKHSVDATYEEQGPSPGFRMELSIFYVVYFVVFPFFFVNIFVALIIITFQEQGDKVMSECSLEKNERACIDFAISAKPLTRYMPQNKQSFQYKTWTFVVSPPFEYFIMAMIALNTVVLMMKFYDAPYEYELMLKCLNIVFTSMFSMECILKIIAFGVLNYFRDAWNVFDFVTVLGSITDILVTEIAETNNFINLSFLRLFRAARLIKLLRQGYTIRILLWTFVQSFKALPYVCLLIAMLFFIYAIIGMQVFGNIALDDDTSINRHNNFRTFLQALMLLFRSATGEAWHEIMLSCLGNRACDPHANASECGSDFAYFYFVSFIFLCSFLMLNLFVAVIMDNFEYLTRDSSILGPHHLDEFIRVWAEYDPAACGRISYNDMFEMLKHMSPPLGLGKKCPARVAYKRLVRMNMPISNEDMTVHFTSTLMALIRTALEIKLAPAGTKQHQCDAELRKEISSVWANLPQKTLDLLVPPHKPDEMTVGKVYAALMIFDFYKQNKTTRDQTHQAPGGLSQMGPVSLFHPLKATLEQTQPAVLRGARVFLRQKSATSLSNGGAIQTQESGIKESLSWGTQRTQDALYEARAPLERGHSAEIPVGQSGTLAVDVQMQNMTLRGPDGEPQPGLESQGRAASMPRLAAETQPAPNASPMKRSISTLAPRPHGTQLCSTVLDRPPPSQASHHHHHRCHRRRDKKQRSLEKGPSLSVDPEGAPSTAAGPGLPHGEGSTACRRDRKQERGRSQERRQPSSSSSEKQRFYSCDRFGSREPPQLMPSLSSHPTSPTAALEPAPHPQGSGSVNGSPLMSTSGASTPGRGGRRQLPQTPLTPRPSITYKTANSSPVHFAEGQSGLPAFSPGRLSRGLSEHNALLQKEPLSQPLAPGSRIGSDPYLGQRLDSEASAHTLPEDTLTFEEAVATNSGRSSRTSYVSSLTSQSHPLRRVPNGYHCTLGLSTGVRARHSYHHPDQDHWC

Target ORF information:

RefSeq Version	XM_006497631.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus calcium channel, voltage-dependent, N type, alpha 1B subunit (Cacna1b), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006497631.3

ORF Insert Sequence:

ATGGTCCGCT TCGGGGACGA GCTAGGCGGC CGCTATGGGG GCACCGGCGG CGGGGAGCGG 
GCTCGGGGTG GCGGGGCCGG CGGGGCGGGT GGCCCGGGCC AGGGGGGTCT GCCGCCGGGC 
CAGCGGGTCC TGTACAAGCA GTCCATTGCG CAGCGCGCAC GGACTATGGC CCTGTACAAC 
CCCATCCCAG TCAAGCAGAA CTGCTTCACC GTCAACCGCT CGCTCTTCGT CTTCAGCGAG 
GACAACGTCG TCCGCAAATA CGCTAAGCGC ATCACCGAAT GGCCGCCCTT CGAATACATG 
ATCCTGGCCA CCATCATCGC CAACTGCATT GTTCTGGCCC TGGAGCAGCA CCTCCCTGAT 
GGGGACAAGA CTCCCATGTC TGAGCGACTA GATGACACGG AGCCTTACTT CATCGGGATC 
TTTTGCTTCG AGGCGGGCAT CAAGATCATA GCCCTGGGCT TTGTTTTCCA CAAGGGCTCC 
TACCTTCGGA ACGGCTGGAA TGTCATGGAC TTCGTGGTGG TCCTCACGGG GATTCTCGCC 
ACAGCTGGAA CTGACTTTGA CCTGCGCACA CTGAGGGCTG TGCGTGTGCT TAGGCCCCTG 
AAGCTGGTGT CTGGAATTCC AAGCTTGCAG GTGGTGCTTA AGTCCATCAT GAAGGCCATG 
GTCCCGCTGC TGCAGATTGG GCTGCTGCTC TTCTTTGCCA TCCTCATGTT TGCCATCATC 
GGCCTCGAAT TCTATATGGG CAAATTCCAT AAGGCCTGTT TCCCCAACAG CACAGATACA 
GAGCCTGTGG GTGACTTTCC CTGTGGCAAA GATCCCCCTG CTCGTCAGTG TGATGGTGAC 
ACCGAATGCC GGGAGTACTG GCCAGGACCC AACTTTGGCA TCACCAATTT TGACAACATC 
CTGTTTGCCA TCTTGACAGT GTTCCAGTGT ATCACCATGG AGGGCTGGAC TGACATCCTC 
TACAATACAA ATGATGCGGC TGGCAACACA TGGAACTGGT TGTACTTCAT CCCCCTCATC 
ATCATTGGCT CCTTCTTCAT GCTCAACCTG GTGCTGGGTG TGCTTTCCGG AGAGTTTGCC 
AAGGAGCGGG AGCGAGTCGA GAACCGCCGC GCCTTCCTGA AGCTCCGCAG GCAGCAGCAG 
ATTGAGCGAG AGCTGAATGG GTACTTGGAG TGGATCTTCA AGGCAGAGGA AGTCATGTTG 
GCAGAGGAGG ACAAGAATGC AGAAGAGAAA TCCCCTTTGG ATGCAGTGTT GAAGAGAGCT 
GCCACCAAGA AGAGCCGAAA TGACCTCATC CATGCAGAAG AGGGGGAGGA CCGGTTTGTA 
GACCTCTGTG CAGTTGGGTC TCCATTTGCT CGTGCCAGCC TCAAGAGTGG GAAGACGGAG 
AGCTCATCGT ACTTCCGGAG AAAGGAGAAG ATGTTCCGGT TCTTTATCCG GCGTATGGTG 
AAAGCACAGA GCTTCTACTG GGTGGTACTG TGTGTGGTGG CCCTGAACAC ACTGTGTGTG 
GCCATGGTGC ACTATAATCA GCCTCAGCGG CTTACCACTG CACTGTACTT TGCAGAGTTT 
GTTTTCCTGG GTCTCTTCCT CACAGAGATG TCCCTGAAGA TGTATGGCCT AGGGCCCAGA 
AGTTACTTCA GGTCTTCCTT CAACTGCTTT GACTTTGGGG TGATTGTGGG GAGTATCTTT 
GAAGTAGTCT GGGCTGCCAT CAAGCCAGGA ACCTCCTTTG GAATCAGTGT GCTGCGGGCT 
CTGCGACTGC TGAGGATATT CAAAGTTACC AAGTATTGGA ACTCTCTGAG GAACCTGGTG 
GTTTCCCTCC TCAATTCCAT GAAGTCCATC ATCAGCCTTC TCTTCCTGCT TTTCCTCTTC 
ATCGTGGTCT TCGCTCTGTT GGGGATGCAG CTGTTTGGGG GACAGTTCAA CTTTCAAGAT 
GAGACTCCAA CCACCAATTT TGATACCTTC CCAGCTGCCA TCCTCACTGT CTTTCAGATC 
CTGACAGGAG AGGATTGGAA TGCCGTAATG TATCATGGGA TTGAGTCGCA AGGTGGAGTC 
AGCAAAGGCA TGTTTTCTTC CTTTTACTTC ATCGTCCTGA CACTGTTTGG AAACTACACC 
CTGCTGAATG TTTTTCTGGC CATTGCTGTG GACAACCTTG CCAATGCCCA GGAGTTGACC 
AAGGATGAAG AGGAGATGGA AGAAGCAGCC AATCAGAAAC TTGCTCTTCA GAAGGCCAAA 
GAAGTAGCTG AAGTCAGCCC CATGTCTGCT GCCAATATCT CCATCGCTGC CAGGCAGCAG 
AACTCGGCCA AGGCGCGCTC AGTATGGGAG CAGCGGGCCA GTCAGCTAAG GCTCCAGAAT 
CTGCGTGCCA GCTGTGAGGC ATTGTACAGT GAGATGGACC CTGAGGAGCG CCTGCGTTAT 
GCCAGCACGC GCCATGTGAG GCCAGACATG AAGACACACA TGGACCGACC CCTAGTGGTG 
GAGCCTGGTC GAGATGGCTT GCGGGGACCC GTTGGGAGCA AGTCAAAGCC TGAAGGCACG 
GAGGCCACAG AAAGCGCGGA CCTACCTCGC AGGCACCACC GGCACCGTGA TAGGGACAAG 
ACCTCAGCCA CAGCACCTGC TGGAGGCGAA CAGGACAGGA CAGAAAGCAC CGAGACCGGG 
GCCCGGGAGG AACGTGCGCG CCCTCGTCGA AGTCACAGCA AGGAGACTCC AGGGGCTGAC 
ACGCAAGTGC GCTGTGAGCG CAGTAGACGT CACCACCGGC GCGGCTCCCC GGAGGAGGCC 
ACTGAACGGG AGCCTCGGCG CCACCGTGCC CACCGGCATG CACAGGACTC AAGCAAGGAG 
GGCACGGCGC CGGTGCTTGT ACCCAAGGGT GAGCGACGAG CAAGACACCG AGGCCCACGC 
ACGGGTCCAC GTGAGGCAGA GAACAACGAG GAGCCCACAC GCAGGCACCG TGCAAGGCAT 
AAGGTGCCAC CCACACTGCA GCCCCCAGAG AGGGAGGCTG CAGAGAAGGA GAGCAACGCG 
GTGGAAGGGG ATAAGGAAAC CCGAAATCAC CAGCCCAAGG AACCTCACTG TGACCTGGAG 
GCCATTGCAG TTACAGGTGT GGGCCCTCTG CACATGCTGC CCAGCACCTG TCTCCAGAAA 
GTGGACGAGC AACCAGAGGA TGCAGACAAC CAGCGTAATG TCACCCGGAT GGGCAGTCAG 
CCCTCAGATC CCAGCACCAC TGTGCATGTC CCAGTGACAC TGACAGGCCC TCCTGGGGAG 
ACCCCTGTAG TTCCCAGTGG TAACATGAAC CTGGAAGGCC AAGCAGAGGG CAAGAAGGAG 
GCAGAGGCGG ATGATGTGCT GAGAAGAGGC CCCAGGCCCA TCGTTCCCTA CAGCTCCATG 
TTTTGTCTCA GCCCCACCAA CCTGCTTCGT CGCTTCTGCC ATTACATTGT GACCATGCGG 
TACTTTGAGA TGGTAATTCT TGTGGTCATT GCCTTGAGCA GCATTGCCCT GGCTGCAGAG 
GATCCTGTGC GGACAGATTC ATTCAGGAAC AACGCTTTAA AGTACATGGA TTACATCTTT 
ACAGGAGTCT TCACCTTTGA AATGGTCATA AAGATGATAG ACTTGGGCTT GCTGCTGCAC 
CCTGGTGCCT ACTTCCGGGA CCTGTGGAAC ATTCTGGACT TCATTGTTGT CAGTGGAGCC 
CTGGTGGCAT TTGCGTTCTC GAGCTTCATG GGAGGATCCA AAGGGAAAGA CATCAATACC 
ATCAAGTCTC TGAGAGTCCT GCGTGTCCTG AGGCCCCTCA AGACCATCAA GCGGCTGCCT 
AAGCTCAAGG CTGTCTTTGA CTGTGTGGTG AACTCCCTGA AGAACGTCTT GAACATCCTG 
ATTGTCTACA TGCTCTTCAT GTTCATATTT GCCGTCATTG CCGTCCAGCT CTTCAAAGGG 
AAGTTCTTTT ACTGTACTGA TGAATCCAAG GAGCTGGAGA GGGACTGCCG GGGTCAGTAT 
TTGGATTATG AGAAGGAAGA AGTAGAAGCC CAGCCAAGGC AGTGGAAGAA ATATGACTTC 
CACTATGACA ATGTTCTCTG GGCCTTGTTG ACGCTGTTCA CAGTGTCCAC GGGAGAGGGG 
TGGCCCATGG TGCTGAAACA CTCTGTGGAT GCCACCTATG AGGAACAGGG GCCCAGTCCC 
GGGTTCCGGA TGGAGCTCTC CATCTTCTAC GTGGTCTACT TTGTGGTCTT CCCTTTTTTC 
TTTGTCAACA TCTTTGTGGC CTTGATCATT ATCACCTTCC AGGAACAGGG AGATAAGGTG 
ATGTCTGAAT GCAGCTTAGA AAAGAATGAG AGGGCTTGCA TTGATTTTGC CATCAGTGCC 
AAGCCCCTGA CACGGTACAT GCCTCAAAAC AAACAGTCGT TCCAGTATAA GACATGGACA 
TTCGTGGTCT CTCCACCCTT TGAGTACTTC ATCATGGCTA TGATAGCCCT CAACACAGTG 
GTGCTGATGA TGAAGTTCTA TGATGCACCT TATGAGTACG AGCTGATGCT GAAATGCCTG 
AACATTGTCT TCACATCCAT GTTCTCGATG GAGTGCATAC TGAAGATCAT CGCCTTTGGG 
GTATTGAACT ACTTCAGAGA TGCCTGGAAC GTCTTTGACT TTGTCACGGT TTTGGGAAGT 
ATTACTGATA TTTTAGTAAC AGAGATAGCG GAAACGAACA ACTTCATCAA CCTAAGCTTC 
CTTCGCCTCT TCCGGGCGGC ACGGCTGATC AAGCTGCTTC GCCAGGGCTA CACCATCCGC 
ATCCTATTGT GGACCTTCGT CCAGTCCTTT AAGGCGCTGC CCTACGTGTG CCTCCTCATT 
GCCATGCTGT TCTTCATCTA CGCCATCATC GGCATGCAGG TTTTTGGAAA CATTGCCCTT 
GATGATGACA CCAGTATCAA CCGACACAAC AACTTCCGGA CATTTCTGCA AGCCTTAATG 
CTATTGTTCA GGAGTGCCAC TGGGGAGGCC TGGCATGAGA TCATGCTGTC TTGTCTGGGC 
AACCGGGCCT GTGACCCACA TGCCAACGCC AGTGAGTGCG GGAGCGACTT TGCCTATTTT 
TATTTTGTCT CCTTCATCTT CCTCTGTTCC TTTCTGATGT TGAACCTCTT TGTTGCTGTA 
ATCATGGACA ATTTTGAGTA CCTCACTCGG GACTCTTCCA TCCTAGGGCC TCACCACTTA 
GACGAATTCA TTCGAGTCTG GGCTGAATAC GACCCAGCTG CGTGTGGGCG CATCAGTTAC 
AATGACATGT TTGAGATGCT GAAACACATG TCCCCACCTC TGGGGTTGGG GAAGAAATGC 
CCGGCTCGAG TTGCATACAA GCGCCTGGTT CGCATGAACA TGCCCATATC CAATGAGGAC 
ATGACGGTGC ACTTTACGTC CACACTGATG GCCCTCATCC GGACAGCACT GGAGATCAAG 
CTTGCCCCAG CGGGGACGAA GCAGCACCAG TGTGATGCTG AGCTGAGAAA AGAGATCTCT 
TCTGTGTGGG CTAATCTGCC CCAGAAGACT CTGGACTTAC TGGTACCACC CCACAAACCT 
GACGAGATGA CAGTGGGGAA GGTCTATGCT GCTCTCATGA TATTTGACTT CTACAAACAG 
AACAAAACCA CCAGAGATCA GACTCACCAA GCTCCCGGAG GCCTGTCCCA GATGGGTCCC 
GTTTCCCTGT TCCACCCTCT GAAGGCCACC CTGGAACAGA CACAGCCCGC TGTGCTTCGA 
GGAGCTCGGG TTTTCCTTCG GCAAAAGAGT GCAACTTCCC TCAGCAATGG GGGTGCCATA 
CAAACCCAGG AAAGTGGCAT CAAGGAGTCG CTGTCCTGGG GCACGCAGAG GACCCAAGAT 
GCACTTTATG AGGCCAGAGC ACCTCTAGAA CGTGGCCATT CTGCAGAGAT CCCTGTGGGG 
CAGTCAGGAA CACTGGCTGT GGATGTCCAG ATGCAGAACA TGACACTGAG AGGACCAGAT 
GGGGAGCCCC AGCCTGGGCT GGAAAGCCAA GGCAGAGCTG CCTCTATGCC ACGCCTAGCG 
GCAGAAACAC AGCCGGCCCC TAATGCCAGC CCCATGAAGC GCTCCATCTC CACACTGGCT 
CCACGCCCAC ATGGGACTCA GCTTTGCAGC ACAGTTCTGG ACCGGCCTCC TCCTAGCCAG 
GCATCACATC ACCACCACCA CCGCTGCCAC CGGCGCAGAG ACAAGAAGCA AAGGTCCCTG 
GAAAAGGGGC CCAGCCTGTC TGTTGACCCA GAAGGTGCAC CAAGCACTGC TGCAGGACCT 
GGTCTGCCCC ATGGAGAAGG ATCCACCGCC TGCCGGCGGG ACCGTAAACA GGAGCGAGGC 
CGGTCCCAGG AGCGGAGGCA GCCCTCATCT TCCTCTTCAG AGAAGCAGCG CTTCTATTCC 
TGTGACCGCT TTGGGAGCCG GGAGCCCCCG CAACTGATGC CCTCACTCAG TAGCCACCCC 
ACATCGCCAA CAGCGGCGCT AGAGCCAGCA CCCCACCCAC AGGGCAGTGG TTCCGTTAAT 
GGGAGCCCCT TGATGTCAAC ATCCGGTGCT AGCACCCCGG GCCGAGGTGG GCGGAGGCAG 
CTCCCCCAGA CTCCTCTGAC CCCACGCCCC AGCATCACCT ACAAGACCGC CAATTCCTCG 
CCTGTCCACT TTGCTGAGGG TCAGAGCGGC CTCCCAGCCT TCTCCCCTGG CCGTCTCAGC 
CGCGGCCTTT CTGAACACAA TGCCCTGCTC CAGAAAGAGC CCCTGAGCCA GCCTCTAGCT 
CCTGGCTCCC GAATTGGCTC TGACCCTTAC CTAGGGCAGC GTCTGGACAG TGAGGCCTCC 
GCCCACACCC TGCCTGAGGA TACACTCACC TTTGAAGAGG CAGTGGCCAC CAACTCTGGC 
CGCTCCTCCA GGACTTCCTA TGTGTCCTCC CTCACTTCCC AATCCCACCC TCTCCGCCGT 
GTACCCAATG GCTATCACTG CACTTTGGGA CTCAGCACTG GCGTCCGGGC GCGGCACAGC 
TACCACCACC CCGATCAGGA CCACTGGTGC TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cacna1b ( XM_006497632.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006497695.1 Mus musculus calcium channel, voltage-dependent, N type, alpha 1B subunit (Cacna1b), transcript variant X10, mRNA.

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CloneID	OMu29263
Clone ID Related Accession (Same CDS sequence)	XM_006497632.3
Accession Version	XM_006497632.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6978bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	voltage-dependent N-type calcium channel subunit alpha-1B isoform X10
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006497632.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTCCGCT TCGGGGACGA GCTAGGCGGC CGCTATGGGG GCACCGGCGG CGGGGAGCGG 
GCTCGGGGTG GCGGGGCCGG CGGGGCGGGT GGCCCGGGCC AGGGGGGTCT GCCGCCGGGC 
CAGCGGGTCC TGTACAAGCA GTCCATTGCG CAGCGCGCAC GGACTATGGC CCTGTACAAC 
CCCATCCCAG TCAAGCAGAA CTGCTTCACC GTCAACCGCT CGCTCTTCGT CTTCAGCGAG 
GACAACGTCG TCCGCAAATA CGCTAAGCGC ATCACCGAAT GGCCGCCCTT CGAATACATG 
ATCCTGGCCA CCATCATCGC CAACTGCATT GTTCTGGCCC TGGAGCAGCA CCTCCCTGAT 
GGGGACAAGA CTCCCATGTC TGAGCGACTA GATGACACGG AGCCTTACTT CATCGGGATC 
TTTTGCTTCG AGGCGGGCAT CAAGATCATA GCCCTGGGCT TTGTTTTCCA CAAGGGCTCC 
TACCTTCGGA ACGGCTGGAA TGTCATGGAC TTCGTGGTGG TCCTCACGGG GATTCTCGCC 
ACAGCTGGAA CTGACTTTGA CCTGCGCACA CTGAGGGCTG TGCGTGTGCT TAGGCCCCTG 
AAGCTGGTGT CTGGAATTCC AAGCTTGCAG GTGGTGCTTA AGTCCATCAT GAAGGCCATG 
GTCCCGCTGC TGCAGATTGG GCTGCTGCTC TTCTTTGCCA TCCTCATGTT TGCCATCATC 
GGCCTCGAAT TCTATATGGG CAAATTCCAT AAGGCCTGTT TCCCCAACAG CACAGATACA 
GAGCCTGTGG GTGACTTTCC CTGTGGCAAA GATCCCCCTG CTCGTCAGTG TGATGGTGAC 
ACCGAATGCC GGGAGTACTG GCCAGGACCC AACTTTGGCA TCACCAATTT TGACAACATC 
CTGTTTGCCA TCTTGACAGT GTTCCAGTGT ATCACCATGG AGGGCTGGAC TGACATCCTC 
TACAATACAA ATGATGCGGC TGGCAACACA TGGAACTGGT TGTACTTCAT CCCCCTCATC 
ATCATTGGCT CCTTCTTCAT GCTCAACCTG GTGCTGGGTG TGCTTTCCGG AGAGTTTGCC 
AAGGAGCGGG AGCGAGTCGA GAACCGCCGC GCCTTCCTGA AGCTCCGCAG GCAGCAGCAG 
ATTGAGCGAG AGCTGAATGG GTACTTGGAG TGGATCTTCA AGGCAGAGGA AGTCATGTTG 
GCAGAGGAGG ACAAGAATGC AGAAGAGAAA TCCCCTTTGG ATGTGTTGAA GAGAGCTGCC 
ACCAAGAAGA GCCGAAATGA CCTCATCCAT GCAGAAGAGG GGGAGGACCG GTTTGTAGAC 
CTCTGTGCAG TTGGGTCTCC ATTTGCTCGT GCCAGCCTCA AGAGTGGGAA GACGGAGAGC 
TCATCGTACT TCCGGAGAAA GGAGAAGATG TTCCGGTTCT TTATCCGGCG TATGGTGAAA 
GCACAGAGCT TCTACTGGGT GGTACTGTGT GTGGTGGCCC TGAACACACT GTGTGTGGCC 
ATGGTGCACT ATAATCAGCC TCAGCGGCTT ACCACTGCAC TGTACTTTGC AGAGTTTGTT 
TTCCTGGGTC TCTTCCTCAC AGAGATGTCC CTGAAGATGT ATGGCCTAGG GCCCAGAAGT 
TACTTCAGGT CTTCCTTCAA CTGCTTTGAC TTTGGGGTGA TTGTGGGGAG TATCTTTGAA 
GTAGTCTGGG CTGCCATCAA GCCAGGAACC TCCTTTGGAA TCAGTGTGCT GCGGGCTCTG 
CGACTGCTGA GGATATTCAA AGTTACCAAG TATTGGAACT CTCTGAGGAA CCTGGTGGTT 
TCCCTCCTCA ATTCCATGAA GTCCATCATC AGCCTTCTCT TCCTGCTTTT CCTCTTCATC 
GTGGTCTTCG CTCTGTTGGG GATGCAGCTG TTTGGGGGAC AGTTCAACTT TCAAGATGAG 
ACTCCAACCA CCAATTTTGA TACCTTCCCA GCTGCCATCC TCACTGTCTT TCAGATCCTG 
ACAGGAGAGG ATTGGAATGC CGTAATGTAT CATGGGATTG AGTCGCAAGG TGGAGTCAGC 
AAAGGCATGT TTTCTTCCTT TTACTTCATC GTCCTGACAC TGTTTGGAAA CTACACCCTG 
CTGAATGTTT TTCTGGCCAT TGCTGTGGAC AACCTTGCCA ATGCCCAGGA GTTGACCAAG 
GATGAAGAGG AGATGGAAGA AGCAGCCAAT CAGAAACTTG CTCTTCAGAA GGCCAAAGAA 
GTAGCTGAAG TCAGCCCCAT GTCTGCTGCC AATATCTCCA TCGCTGCCAG GCAGCAGAAC 
TCGGCCAAGG CGCGCTCAGT ATGGGAGCAG CGGGCCAGTC AGCTAAGGCT CCAGAATCTG 
CGTGCCAGCT GTGAGGCATT GTACAGTGAG ATGGACCCTG AGGAGCGCCT GCGTTATGCC 
AGCACGCGCC ATGTGAGGCC AGACATGAAG ACACACATGG ACCGACCCCT AGTGGTGGAG 
CCTGGTCGAG ATGGCTTGCG GGGACCCGTT GGGAGCAAGT CAAAGCCTGA AGGCACGGAG 
GCCACAGAAA GCGCGGACCT ACCTCGCAGG CACCACCGGC ACCGTGATAG GGACAAGACC 
TCAGCCACAG CACCTGCTGG AGGCGAACAG GACAGGACAG AAAGCACCGA GACCGGGGCC 
CGGGAGGAAC GTGCGCGCCC TCGTCGAAGT CACAGCAAGG AGACTCCAGG GGCTGACACG 
CAAGTGCGCT GTGAGCGCAG TAGACGTCAC CACCGGCGCG GCTCCCCGGA GGAGGCCACT 
GAACGGGAGC CTCGGCGCCA CCGTGCCCAC CGGCATGCAC AGGACTCAAG CAAGGAGGGC 
ACGGCGCCGG TGCTTGTACC CAAGGGTGAG CGACGAGCAA GACACCGAGG CCCACGCACG 
GGTCCACGTG AGGCAGAGAA CAACGAGGAG CCCACACGCA GGCACCGTGC AAGGCATAAG 
GTGCCACCCA CACTGCAGCC CCCAGAGAGG GAGGCTGCAG AGAAGGAGAG CAACGCGGTG 
GAAGGGGATA AGGAAACCCG AAATCACCAG CCCAAGGAAC CTCACTGTGA CCTGGAGGCC 
ATTGCAGTTA CAGGTGTGGG CCCTCTGCAC ATGCTGCCCA GCACCTGTCT CCAGAAAGTG 
GACGAGCAAC CAGAGGATGC AGACAACCAG CGTAATGTCA CCCGGATGGG CAGTCAGCCC 
TCAGATCCCA GCACCACTGT GCATGTCCCA GTGACACTGA CAGGCCCTCC TGGGGAGACC 
CCTGTAGTTC CCAGTGGTAA CATGAACCTG GAAGGCCAAG CAGAGGGCAA GAAGGAGGCA 
GAGGCGGATG ATGTGCTGAG AAGAGGCCCC AGGCCCATCG TTCCCTACAG CTCCATGTTT 
TGTCTCAGCC CCACCAACCT GCTTCGTCGC TTCTGCCATT ACATTGTGAC CATGCGGTAC 
TTTGAGATGG TAATTCTTGT GGTCATTGCC TTGAGCAGCA TTGCCCTGGC TGCAGAGGAT 
CCTGTGCGGA CAGATTCATT CAGGAACAAC GCTTTAAAGT ACATGGATTA CATCTTTACA 
GGAGTCTTCA CCTTTGAAAT GGTCATAAAG ATGATAGACT TGGGCTTGCT GCTGCACCCT 
GGTGCCTACT TCCGGGACCT GTGGAACATT CTGGACTTCA TTGTTGTCAG TGGAGCCCTG 
GTGGCATTTG CGTTCTCAGG ATCCAAAGGG AAAGACATCA ATACCATCAA GTCTCTGAGA 
GTCCTGCGTG TCCTGAGGCC CCTCAAGACC ATCAAGCGGC TGCCTAAGCT CAAGGCTGTC 
TTTGACTGTG TGGTGAACTC CCTGAAGAAC GTCTTGAACA TCCTGATTGT CTACATGCTC 
TTCATGTTCA TATTTGCCGT CATTGCCGTC CAGCTCTTCA AAGGGAAGTT CTTTTACTGT 
ACTGATGAAT CCAAGGAGCT GGAGAGGGAC TGCCGGGGTC AGTATTTGGA TTATGAGAAG 
GAAGAAGTAG AAGCCCAGCC AAGGCAGTGG AAGAAATATG ACTTCCACTA TGACAATGTT 
CTCTGGGCCT TGTTGACGCT GTTCACAGTG TCCACGGGAG AGGGGTGGCC CATGGTGCTG 
AAACACTCTG TGGATGCCAC CTATGAGGAA CAGGGGCCCA GTCCCGGGTT CCGGATGGAG 
CTCTCCATCT TCTACGTGGT CTACTTTGTG GTCTTCCCTT TTTTCTTTGT CAACATCTTT 
GTGGCCTTGA TCATTATCAC CTTCCAGGAA CAGGGAGATA AGGTGATGTC TGAATGCAGC 
TTAGAAAAGA ATGAGAGGGC TTGCATTGAT TTTGCCATCA GTGCCAAGCC CCTGACACGG 
TACATGCCTC AAAACAAACA GTCGTTCCAG TATAAGACAT GGACATTCGT GGTCTCTCCA 
CCCTTTGAGT ACTTCATCAT GGCTATGATA GCCCTCAACA CAGTGGTGCT GATGATGAAG 
TTCTATGATG CACCTTATGA GTACGAGCTG ATGCTGAAAT GCCTGAACAT TGTCTTCACA 
TCCATGTTCT CGATGGAGTG CATACTGAAG ATCATCGCCT TTGGGGTATT GAACTACTTC 
AGAGATGCCT GGAACGTCTT TGACTTTGTC ACGGTTTTGG GAAGTATTAC TGATATTTTA 
GTAACAGAGA TAGCGGAAAC GAACAACTTC ATCAACCTAA GCTTCCTTCG CCTCTTCCGG 
GCGGCACGGC TGATCAAGCT GCTTCGCCAG GGCTACACCA TCCGCATCCT ATTGTGGACC 
TTCGTCCAGT CCTTTAAGGC GCTGCCCTAC GTGTGCCTCC TCATTGCCAT GCTGTTCTTC 
ATCTACGCCA TCATCGGCAT GCAGGTTTTT GGAAACATTG CCCTTGATGA TGACACCAGT 
ATCAACCGAC ACAACAACTT CCGGACATTT CTGCAAGCCT TAATGCTATT GTTCAGGAGT 
GCCACTGGGG AGGCCTGGCA TGAGATCATG CTGTCTTGTC TGGGCAACCG GGCCTGTGAC 
CCACATGCCA ACGCCAGTGA GTGCGGGAGC GACTTTGCCT ATTTTTATTT TGTCTCCTTC 
ATCTTCCTCT GTTCCTTTCT GATGTTGAAC CTCTTTGTTG CTGTAATCAT GGACAATTTT 
GAGTACCTCA CTCGGGACTC TTCCATCCTA GGGCCTCACC ACTTAGACGA ATTCATTCGA 
GTCTGGGCTG AATACGACCC AGCTGCGTGT GGGCGCATCA GTTACAATGA CATGTTTGAG 
ATGCTGAAAC ACATGTCCCC ACCTCTGGGG TTGGGGAAGA AATGCCCGGC TCGAGTTGCA 
TACAAGCGCC TGGTTCGCAT GAACATGCCC ATATCCAATG AGGACATGAC GGTGCACTTT 
ACGTCCACAC TGATGGCCCT CATCCGGACA GCACTGGAGA TCAAGCTTGC CCCAGCGGGG 
ACGAAGCAGC ACCAGTGTGA TGCTGAGCTG AGAAAAGAGA TCTCTTCTGT GTGGGCTAAT 
CTGCCCCAGA AGACTCTGGA CTTACTGGTA CCACCCCACA AACCTGACGA GATGACAGTG 
GGGAAGGTCT ATGCTGCTCT CATGATATTT GACTTCTACA AACAGAACAA AACCACCAGA 
GATCAGACTC ACCAAGCTCC CGGAGGCCTG TCCCAGATGG GTCCCGTTTC CCTGTTCCAC 
CCTCTGAAGG CCACCCTGGA ACAGACACAG CCCGCTGTGC TTCGAGGAGC TCGGGTTTTC 
CTTCGGCAAA AGAGTGCAAC TTCCCTCAGC AATGGGGGTG CCATACAAAC CCAGGAAAGT 
GGCATCAAGG AGTCGCTGTC CTGGGGCACG CAGAGGACCC AAGATGCACT TTATGAGGCC 
AGAGCACCTC TAGAACGTGG CCATTCTGCA GAGATCCCTG TGGGGCAGTC AGGAACACTG 
GCTGTGGATG TCCAGATGCA GAACATGACA CTGAGAGGAC CAGATGGGGA GCCCCAGCCT 
GGGCTGGAAA GCCAAGGCAG AGCTGCCTCT ATGCCACGCC TAGCGGCAGA AACACAGCCG 
GCCCCTAATG CCAGCCCCAT GAAGCGCTCC ATCTCCACAC TGGCTCCACG CCCACATGGG 
ACTCAGCTTT GCAGCACAGT TCTGGACCGG CCTCCTCCTA GCCAGGCATC ACATCACCAC 
CACCACCGCT GCCACCGGCG CAGAGACAAG AAGCAAAGGT CCCTGGAAAA GGGGCCCAGC 
CTGTCTGTTG ACCCAGAAGG TGCACCAAGC ACTGCTGCAG GACCTGGTCT GCCCCATGGA 
GAAGGATCCA CCGCCTGCCG GCGGGACCGT AAACAGGAGC GAGGCCGGTC CCAGGAGCGG 
AGGCAGCCCT CATCTTCCTC TTCAGAGAAG CAGCGCTTCT ATTCCTGTGA CCGCTTTGGG 
AGCCGGGAGC CCCCGCAACT GATGCCCTCA CTCAGTAGCC ACCCCACATC GCCAACAGCG 
GCGCTAGAGC CAGCACCCCA CCCACAGGGC AGTGGTTCCG TTAATGGGAG CCCCTTGATG 
TCAACATCCG GTGCTAGCAC CCCGGGCCGA GGTGGGCGGA GGCAGCTCCC CCAGACTCCT 
CTGACCCCAC GCCCCAGCAT CACCTACAAG ACCGCCAATT CCTCGCCTGT CCACTTTGCT 
GAGGGTCAGA GCGGCCTCCC AGCCTTCTCC CCTGGCCGTC TCAGCCGCGG CCTTTCTGAA 
CACAATGCCC TGCTCCAGAA AGAGCCCCTG AGCCAGCCTC TAGCTCCTGG CTCCCGAATT 
GGCTCTGACC CTTACCTAGG GCAGCGTCTG GACAGTGAGG CCTCCGCCCA CACCCTGCCT 
GAGGATACAC TCACCTTTGA AGAGGCAGTG GCCACCAACT CTGGCCGCTC CTCCAGGACT 
TCCTATGTGT CCTCCCTCAC TTCCCAATCC CACCCTCTCC GCCGTGTACC CAATGGCTAT 
CACTGCACTT TGGGACTCAG CACTGGCGTC CGGGCGCGGC ACAGCTACCA CCACCCCGAT 
CAGGACCACT GGTGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006497695.1
CDS	146..7123
Translation	MVRFGDELGGRYGGTGGGERARGGGAGGAGGPGQGGLPPGQRVLYKQSIAQRARTMALYNPIPVKQNCFTVNRSLFVFSEDNVVRKYAKRITEWPPFEYMILATIIANCIVLALEQHLPDGDKTPMSERLDDTEPYFIGIFCFEAGIKIIALGFVFHKGSYLRNGWNVMDFVVVLTGILATAGTDFDLRTLRAVRVLRPLKLVSGIPSLQVVLKSIMKAMVPLLQIGLLLFFAILMFAIIGLEFYMGKFHKACFPNSTDTEPVGDFPCGKDPPARQCDGDTECREYWPGPNFGITNFDNILFAILTVFQCITMEGWTDILYNTNDAAGNTWNWLYFIPLIIIGSFFMLNLVLGVLSGEFAKERERVENRRAFLKLRRQQQIERELNGYLEWIFKAEEVMLAEEDKNAEEKSPLDVLKRAATKKSRNDLIHAEEGEDRFVDLCAVGSPFARASLKSGKTESSSYFRRKEKMFRFFIRRMVKAQSFYWVVLCVVALNTLCVAMVHYNQPQRLTTALYFAEFVFLGLFLTEMSLKMYGLGPRSYFRSSFNCFDFGVIVGSIFEVVWAAIKPGTSFGISVLRALRLLRIFKVTKYWNSLRNLVVSLLNSMKSIISLLFLLFLFIVVFALLGMQLFGGQFNFQDETPTTNFDTFPAAILTVFQILTGEDWNAVMYHGIESQGGVSKGMFSSFYFIVLTLFGNYTLLNVFLAIAVDNLANAQELTKDEEEMEEAANQKLALQKAKEVAEVSPMSAANISIAARQQNSAKARSVWEQRASQLRLQNLRASCEALYSEMDPEERLRYASTRHVRPDMKTHMDRPLVVEPGRDGLRGPVGSKSKPEGTEATESADLPRRHHRHRDRDKTSATAPAGGEQDRTESTETGAREERARPRRSHSKETPGADTQVRCERSRRHHRRGSPEEATEREPRRHRAHRHAQDSSKEGTAPVLVPKGERRARHRGPRTGPREAENNEEPTRRHRARHKVPPTLQPPEREAAEKESNAVEGDKETRNHQPKEPHCDLEAIAVTGVGPLHMLPSTCLQKVDEQPEDADNQRNVTRMGSQPSDPSTTVHVPVTLTGPPGETPVVPSGNMNLEGQAEGKKEAEADDVLRRGPRPIVPYSSMFCLSPTNLLRRFCHYIVTMRYFEMVILVVIALSSIALAAEDPVRTDSFRNNALKYMDYIFTGVFTFEMVIKMIDLGLLLHPGAYFRDLWNILDFIVVSGALVAFAFSGSKGKDINTIKSLRVLRVLRPLKTIKRLPKLKAVFDCVVNSLKNVLNILIVYMLFMFIFAVIAVQLFKGKFFYCTDESKELERDCRGQYLDYEKEEVEAQPRQWKKYDFHYDNVLWALLTLFTVSTGEGWPMVLKHSVDATYEEQGPSPGFRMELSIFYVVYFVVFPFFFVNIFVALIIITFQEQGDKVMSECSLEKNERACIDFAISAKPLTRYMPQNKQSFQYKTWTFVVSPPFEYFIMAMIALNTVVLMMKFYDAPYEYELMLKCLNIVFTSMFSMECILKIIAFGVLNYFRDAWNVFDFVTVLGSITDILVTEIAETNNFINLSFLRLFRAARLIKLLRQGYTIRILLWTFVQSFKALPYVCLLIAMLFFIYAIIGMQVFGNIALDDDTSINRHNNFRTFLQALMLLFRSATGEAWHEIMLSCLGNRACDPHANASECGSDFAYFYFVSFIFLCSFLMLNLFVAVIMDNFEYLTRDSSILGPHHLDEFIRVWAEYDPAACGRISYNDMFEMLKHMSPPLGLGKKCPARVAYKRLVRMNMPISNEDMTVHFTSTLMALIRTALEIKLAPAGTKQHQCDAELRKEISSVWANLPQKTLDLLVPPHKPDEMTVGKVYAALMIFDFYKQNKTTRDQTHQAPGGLSQMGPVSLFHPLKATLEQTQPAVLRGARVFLRQKSATSLSNGGAIQTQESGIKESLSWGTQRTQDALYEARAPLERGHSAEIPVGQSGTLAVDVQMQNMTLRGPDGEPQPGLESQGRAASMPRLAAETQPAPNASPMKRSISTLAPRPHGTQLCSTVLDRPPPSQASHHHHHRCHRRRDKKQRSLEKGPSLSVDPEGAPSTAAGPGLPHGEGSTACRRDRKQERGRSQERRQPSSSSSEKQRFYSCDRFGSREPPQLMPSLSSHPTSPTAALEPAPHPQGSGSVNGSPLMSTSGASTPGRGGRRQLPQTPLTPRPSITYKTANSSPVHFAEGQSGLPAFSPGRLSRGLSEHNALLQKEPLSQPLAPGSRIGSDPYLGQRLDSEASAHTLPEDTLTFEEAVATNSGRSSRTSYVSSLTSQSHPLRRVPNGYHCTLGLSTGVRARHSYHHPDQDHWC

Target ORF information:

RefSeq Version	XM_006497632.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus calcium channel, voltage-dependent, N type, alpha 1B subunit (Cacna1b), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006497632.3

ORF Insert Sequence:

ATGGTCCGCT TCGGGGACGA GCTAGGCGGC CGCTATGGGG GCACCGGCGG CGGGGAGCGG 
GCTCGGGGTG GCGGGGCCGG CGGGGCGGGT GGCCCGGGCC AGGGGGGTCT GCCGCCGGGC 
CAGCGGGTCC TGTACAAGCA GTCCATTGCG CAGCGCGCAC GGACTATGGC CCTGTACAAC 
CCCATCCCAG TCAAGCAGAA CTGCTTCACC GTCAACCGCT CGCTCTTCGT CTTCAGCGAG 
GACAACGTCG TCCGCAAATA CGCTAAGCGC ATCACCGAAT GGCCGCCCTT CGAATACATG 
ATCCTGGCCA CCATCATCGC CAACTGCATT GTTCTGGCCC TGGAGCAGCA CCTCCCTGAT 
GGGGACAAGA CTCCCATGTC TGAGCGACTA GATGACACGG AGCCTTACTT CATCGGGATC 
TTTTGCTTCG AGGCGGGCAT CAAGATCATA GCCCTGGGCT TTGTTTTCCA CAAGGGCTCC 
TACCTTCGGA ACGGCTGGAA TGTCATGGAC TTCGTGGTGG TCCTCACGGG GATTCTCGCC 
ACAGCTGGAA CTGACTTTGA CCTGCGCACA CTGAGGGCTG TGCGTGTGCT TAGGCCCCTG 
AAGCTGGTGT CTGGAATTCC AAGCTTGCAG GTGGTGCTTA AGTCCATCAT GAAGGCCATG 
GTCCCGCTGC TGCAGATTGG GCTGCTGCTC TTCTTTGCCA TCCTCATGTT TGCCATCATC 
GGCCTCGAAT TCTATATGGG CAAATTCCAT AAGGCCTGTT TCCCCAACAG CACAGATACA 
GAGCCTGTGG GTGACTTTCC CTGTGGCAAA GATCCCCCTG CTCGTCAGTG TGATGGTGAC 
ACCGAATGCC GGGAGTACTG GCCAGGACCC AACTTTGGCA TCACCAATTT TGACAACATC 
CTGTTTGCCA TCTTGACAGT GTTCCAGTGT ATCACCATGG AGGGCTGGAC TGACATCCTC 
TACAATACAA ATGATGCGGC TGGCAACACA TGGAACTGGT TGTACTTCAT CCCCCTCATC 
ATCATTGGCT CCTTCTTCAT GCTCAACCTG GTGCTGGGTG TGCTTTCCGG AGAGTTTGCC 
AAGGAGCGGG AGCGAGTCGA GAACCGCCGC GCCTTCCTGA AGCTCCGCAG GCAGCAGCAG 
ATTGAGCGAG AGCTGAATGG GTACTTGGAG TGGATCTTCA AGGCAGAGGA AGTCATGTTG 
GCAGAGGAGG ACAAGAATGC AGAAGAGAAA TCCCCTTTGG ATGTGTTGAA GAGAGCTGCC 
ACCAAGAAGA GCCGAAATGA CCTCATCCAT GCAGAAGAGG GGGAGGACCG GTTTGTAGAC 
CTCTGTGCAG TTGGGTCTCC ATTTGCTCGT GCCAGCCTCA AGAGTGGGAA GACGGAGAGC 
TCATCGTACT TCCGGAGAAA GGAGAAGATG TTCCGGTTCT TTATCCGGCG TATGGTGAAA 
GCACAGAGCT TCTACTGGGT GGTACTGTGT GTGGTGGCCC TGAACACACT GTGTGTGGCC 
ATGGTGCACT ATAATCAGCC TCAGCGGCTT ACCACTGCAC TGTACTTTGC AGAGTTTGTT 
TTCCTGGGTC TCTTCCTCAC AGAGATGTCC CTGAAGATGT ATGGCCTAGG GCCCAGAAGT 
TACTTCAGGT CTTCCTTCAA CTGCTTTGAC TTTGGGGTGA TTGTGGGGAG TATCTTTGAA 
GTAGTCTGGG CTGCCATCAA GCCAGGAACC TCCTTTGGAA TCAGTGTGCT GCGGGCTCTG 
CGACTGCTGA GGATATTCAA AGTTACCAAG TATTGGAACT CTCTGAGGAA CCTGGTGGTT 
TCCCTCCTCA ATTCCATGAA GTCCATCATC AGCCTTCTCT TCCTGCTTTT CCTCTTCATC 
GTGGTCTTCG CTCTGTTGGG GATGCAGCTG TTTGGGGGAC AGTTCAACTT TCAAGATGAG 
ACTCCAACCA CCAATTTTGA TACCTTCCCA GCTGCCATCC TCACTGTCTT TCAGATCCTG 
ACAGGAGAGG ATTGGAATGC CGTAATGTAT CATGGGATTG AGTCGCAAGG TGGAGTCAGC 
AAAGGCATGT TTTCTTCCTT TTACTTCATC GTCCTGACAC TGTTTGGAAA CTACACCCTG 
CTGAATGTTT TTCTGGCCAT TGCTGTGGAC AACCTTGCCA ATGCCCAGGA GTTGACCAAG 
GATGAAGAGG AGATGGAAGA AGCAGCCAAT CAGAAACTTG CTCTTCAGAA GGCCAAAGAA 
GTAGCTGAAG TCAGCCCCAT GTCTGCTGCC AATATCTCCA TCGCTGCCAG GCAGCAGAAC 
TCGGCCAAGG CGCGCTCAGT ATGGGAGCAG CGGGCCAGTC AGCTAAGGCT CCAGAATCTG 
CGTGCCAGCT GTGAGGCATT GTACAGTGAG ATGGACCCTG AGGAGCGCCT GCGTTATGCC 
AGCACGCGCC ATGTGAGGCC AGACATGAAG ACACACATGG ACCGACCCCT AGTGGTGGAG 
CCTGGTCGAG ATGGCTTGCG GGGACCCGTT GGGAGCAAGT CAAAGCCTGA AGGCACGGAG 
GCCACAGAAA GCGCGGACCT ACCTCGCAGG CACCACCGGC ACCGTGATAG GGACAAGACC 
TCAGCCACAG CACCTGCTGG AGGCGAACAG GACAGGACAG AAAGCACCGA GACCGGGGCC 
CGGGAGGAAC GTGCGCGCCC TCGTCGAAGT CACAGCAAGG AGACTCCAGG GGCTGACACG 
CAAGTGCGCT GTGAGCGCAG TAGACGTCAC CACCGGCGCG GCTCCCCGGA GGAGGCCACT 
GAACGGGAGC CTCGGCGCCA CCGTGCCCAC CGGCATGCAC AGGACTCAAG CAAGGAGGGC 
ACGGCGCCGG TGCTTGTACC CAAGGGTGAG CGACGAGCAA GACACCGAGG CCCACGCACG 
GGTCCACGTG AGGCAGAGAA CAACGAGGAG CCCACACGCA GGCACCGTGC AAGGCATAAG 
GTGCCACCCA CACTGCAGCC CCCAGAGAGG GAGGCTGCAG AGAAGGAGAG CAACGCGGTG 
GAAGGGGATA AGGAAACCCG AAATCACCAG CCCAAGGAAC CTCACTGTGA CCTGGAGGCC 
ATTGCAGTTA CAGGTGTGGG CCCTCTGCAC ATGCTGCCCA GCACCTGTCT CCAGAAAGTG 
GACGAGCAAC CAGAGGATGC AGACAACCAG CGTAATGTCA CCCGGATGGG CAGTCAGCCC 
TCAGATCCCA GCACCACTGT GCATGTCCCA GTGACACTGA CAGGCCCTCC TGGGGAGACC 
CCTGTAGTTC CCAGTGGTAA CATGAACCTG GAAGGCCAAG CAGAGGGCAA GAAGGAGGCA 
GAGGCGGATG ATGTGCTGAG AAGAGGCCCC AGGCCCATCG TTCCCTACAG CTCCATGTTT 
TGTCTCAGCC CCACCAACCT GCTTCGTCGC TTCTGCCATT ACATTGTGAC CATGCGGTAC 
TTTGAGATGG TAATTCTTGT GGTCATTGCC TTGAGCAGCA TTGCCCTGGC TGCAGAGGAT 
CCTGTGCGGA CAGATTCATT CAGGAACAAC GCTTTAAAGT ACATGGATTA CATCTTTACA 
GGAGTCTTCA CCTTTGAAAT GGTCATAAAG ATGATAGACT TGGGCTTGCT GCTGCACCCT 
GGTGCCTACT TCCGGGACCT GTGGAACATT CTGGACTTCA TTGTTGTCAG TGGAGCCCTG 
GTGGCATTTG CGTTCTCAGG ATCCAAAGGG AAAGACATCA ATACCATCAA GTCTCTGAGA 
GTCCTGCGTG TCCTGAGGCC CCTCAAGACC ATCAAGCGGC TGCCTAAGCT CAAGGCTGTC 
TTTGACTGTG TGGTGAACTC CCTGAAGAAC GTCTTGAACA TCCTGATTGT CTACATGCTC 
TTCATGTTCA TATTTGCCGT CATTGCCGTC CAGCTCTTCA AAGGGAAGTT CTTTTACTGT 
ACTGATGAAT CCAAGGAGCT GGAGAGGGAC TGCCGGGGTC AGTATTTGGA TTATGAGAAG 
GAAGAAGTAG AAGCCCAGCC AAGGCAGTGG AAGAAATATG ACTTCCACTA TGACAATGTT 
CTCTGGGCCT TGTTGACGCT GTTCACAGTG TCCACGGGAG AGGGGTGGCC CATGGTGCTG 
AAACACTCTG TGGATGCCAC CTATGAGGAA CAGGGGCCCA GTCCCGGGTT CCGGATGGAG 
CTCTCCATCT TCTACGTGGT CTACTTTGTG GTCTTCCCTT TTTTCTTTGT CAACATCTTT 
GTGGCCTTGA TCATTATCAC CTTCCAGGAA CAGGGAGATA AGGTGATGTC TGAATGCAGC 
TTAGAAAAGA ATGAGAGGGC TTGCATTGAT TTTGCCATCA GTGCCAAGCC CCTGACACGG 
TACATGCCTC AAAACAAACA GTCGTTCCAG TATAAGACAT GGACATTCGT GGTCTCTCCA 
CCCTTTGAGT ACTTCATCAT GGCTATGATA GCCCTCAACA CAGTGGTGCT GATGATGAAG 
TTCTATGATG CACCTTATGA GTACGAGCTG ATGCTGAAAT GCCTGAACAT TGTCTTCACA 
TCCATGTTCT CGATGGAGTG CATACTGAAG ATCATCGCCT TTGGGGTATT GAACTACTTC 
AGAGATGCCT GGAACGTCTT TGACTTTGTC ACGGTTTTGG GAAGTATTAC TGATATTTTA 
GTAACAGAGA TAGCGGAAAC GAACAACTTC ATCAACCTAA GCTTCCTTCG CCTCTTCCGG 
GCGGCACGGC TGATCAAGCT GCTTCGCCAG GGCTACACCA TCCGCATCCT ATTGTGGACC 
TTCGTCCAGT CCTTTAAGGC GCTGCCCTAC GTGTGCCTCC TCATTGCCAT GCTGTTCTTC 
ATCTACGCCA TCATCGGCAT GCAGGTTTTT GGAAACATTG CCCTTGATGA TGACACCAGT 
ATCAACCGAC ACAACAACTT CCGGACATTT CTGCAAGCCT TAATGCTATT GTTCAGGAGT 
GCCACTGGGG AGGCCTGGCA TGAGATCATG CTGTCTTGTC TGGGCAACCG GGCCTGTGAC 
CCACATGCCA ACGCCAGTGA GTGCGGGAGC GACTTTGCCT ATTTTTATTT TGTCTCCTTC 
ATCTTCCTCT GTTCCTTTCT GATGTTGAAC CTCTTTGTTG CTGTAATCAT GGACAATTTT 
GAGTACCTCA CTCGGGACTC TTCCATCCTA GGGCCTCACC ACTTAGACGA ATTCATTCGA 
GTCTGGGCTG AATACGACCC AGCTGCGTGT GGGCGCATCA GTTACAATGA CATGTTTGAG 
ATGCTGAAAC ACATGTCCCC ACCTCTGGGG TTGGGGAAGA AATGCCCGGC TCGAGTTGCA 
TACAAGCGCC TGGTTCGCAT GAACATGCCC ATATCCAATG AGGACATGAC GGTGCACTTT 
ACGTCCACAC TGATGGCCCT CATCCGGACA GCACTGGAGA TCAAGCTTGC CCCAGCGGGG 
ACGAAGCAGC ACCAGTGTGA TGCTGAGCTG AGAAAAGAGA TCTCTTCTGT GTGGGCTAAT 
CTGCCCCAGA AGACTCTGGA CTTACTGGTA CCACCCCACA AACCTGACGA GATGACAGTG 
GGGAAGGTCT ATGCTGCTCT CATGATATTT GACTTCTACA AACAGAACAA AACCACCAGA 
GATCAGACTC ACCAAGCTCC CGGAGGCCTG TCCCAGATGG GTCCCGTTTC CCTGTTCCAC 
CCTCTGAAGG CCACCCTGGA ACAGACACAG CCCGCTGTGC TTCGAGGAGC TCGGGTTTTC 
CTTCGGCAAA AGAGTGCAAC TTCCCTCAGC AATGGGGGTG CCATACAAAC CCAGGAAAGT 
GGCATCAAGG AGTCGCTGTC CTGGGGCACG CAGAGGACCC AAGATGCACT TTATGAGGCC 
AGAGCACCTC TAGAACGTGG CCATTCTGCA GAGATCCCTG TGGGGCAGTC AGGAACACTG 
GCTGTGGATG TCCAGATGCA GAACATGACA CTGAGAGGAC CAGATGGGGA GCCCCAGCCT 
GGGCTGGAAA GCCAAGGCAG AGCTGCCTCT ATGCCACGCC TAGCGGCAGA AACACAGCCG 
GCCCCTAATG CCAGCCCCAT GAAGCGCTCC ATCTCCACAC TGGCTCCACG CCCACATGGG 
ACTCAGCTTT GCAGCACAGT TCTGGACCGG CCTCCTCCTA GCCAGGCATC ACATCACCAC 
CACCACCGCT GCCACCGGCG CAGAGACAAG AAGCAAAGGT CCCTGGAAAA GGGGCCCAGC 
CTGTCTGTTG ACCCAGAAGG TGCACCAAGC ACTGCTGCAG GACCTGGTCT GCCCCATGGA 
GAAGGATCCA CCGCCTGCCG GCGGGACCGT AAACAGGAGC GAGGCCGGTC CCAGGAGCGG 
AGGCAGCCCT CATCTTCCTC TTCAGAGAAG CAGCGCTTCT ATTCCTGTGA CCGCTTTGGG 
AGCCGGGAGC CCCCGCAACT GATGCCCTCA CTCAGTAGCC ACCCCACATC GCCAACAGCG 
GCGCTAGAGC CAGCACCCCA CCCACAGGGC AGTGGTTCCG TTAATGGGAG CCCCTTGATG 
TCAACATCCG GTGCTAGCAC CCCGGGCCGA GGTGGGCGGA GGCAGCTCCC CCAGACTCCT 
CTGACCCCAC GCCCCAGCAT CACCTACAAG ACCGCCAATT CCTCGCCTGT CCACTTTGCT 
GAGGGTCAGA GCGGCCTCCC AGCCTTCTCC CCTGGCCGTC TCAGCCGCGG CCTTTCTGAA 
CACAATGCCC TGCTCCAGAA AGAGCCCCTG AGCCAGCCTC TAGCTCCTGG CTCCCGAATT 
GGCTCTGACC CTTACCTAGG GCAGCGTCTG GACAGTGAGG CCTCCGCCCA CACCCTGCCT 
GAGGATACAC TCACCTTTGA AGAGGCAGTG GCCACCAACT CTGGCCGCTC CTCCAGGACT 
TCCTATGTGT CCTCCCTCAC TTCCCAATCC CACCCTCTCC GCCGTGTACC CAATGGCTAT 
CACTGCACTT TGGGACTCAG CACTGGCGTC CGGGCGCGGC ACAGCTACCA CCACCCCGAT 
CAGGACCACT GGTGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cacna1b ( XM_006497633.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006497696.1 Mus musculus calcium channel, voltage-dependent, N type, alpha 1B subunit (Cacna1b), transcript variant X11, mRNA.

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CloneID	OMu29264
Clone ID Related Accession (Same CDS sequence)	XM_006497633.3
Accession Version	XM_006497633.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6975bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	voltage-dependent N-type calcium channel subunit alpha-1B isoform X11
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006497633.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTCCGCT TCGGGGACGA GCTAGGCGGC CGCTATGGGG GCACCGGCGG CGGGGAGCGG 
GCTCGGGGTG GCGGGGCCGG CGGGGCGGGT GGCCCGGGCC AGGGGGGTCT GCCGCCGGGC 
CAGCGGGTCC TGTACAAGCA GTCCATTGCG CAGCGCGCAC GGACTATGGC CCTGTACAAC 
CCCATCCCAG TCAAGCAGAA CTGCTTCACC GTCAACCGCT CGCTCTTCGT CTTCAGCGAG 
GACAACGTCG TCCGCAAATA CGCTAAGCGC ATCACCGAAT GGCCGCCCTT CGAATACATG 
ATCCTGGCCA CCATCATCGC CAACTGCATT GTTCTGGCCC TGGAGCAGCA CCTCCCTGAT 
GGGGACAAGA CTCCCATGTC TGAGCGACTA GATGACACGG AGCCTTACTT CATCGGGATC 
TTTTGCTTCG AGGCGGGCAT CAAGATCATA GCCCTGGGCT TTGTTTTCCA CAAGGGCTCC 
TACCTTCGGA ACGGCTGGAA TGTCATGGAC TTCGTGGTGG TCCTCACGGG GATTCTCGCC 
ACAGCTGGAA CTGACTTTGA CCTGCGCACA CTGAGGGCTG TGCGTGTGCT TAGGCCCCTG 
AAGCTGGTGT CTGGAATTCC AAGCTTGCAG GTGGTGCTTA AGTCCATCAT GAAGGCCATG 
GTCCCGCTGC TGCAGATTGG GCTGCTGCTC TTCTTTGCCA TCCTCATGTT TGCCATCATC 
GGCCTCGAAT TCTATATGGG CAAATTCCAT AAGGCCTGTT TCCCCAACAG CACAGATACA 
GAGCCTGTGG GTGACTTTCC CTGTGGCAAA GATCCCCCTG CTCGTCAGTG TGATGGTGAC 
ACCGAATGCC GGGAGTACTG GCCAGGACCC AACTTTGGCA TCACCAATTT TGACAACATC 
CTGTTTGCCA TCTTGACAGT GTTCCAGTGT ATCACCATGG AGGGCTGGAC TGACATCCTC 
TACAATACAA ATGATGCGGC TGGCAACACA TGGAACTGGT TGTACTTCAT CCCCCTCATC 
ATCATTGGCT CCTTCTTCAT GCTCAACCTG GTGCTGGGTG TGCTTTCCGG AGAGTTTGCC 
AAGGAGCGGG AGCGAGTCGA GAACCGCCGC GCCTTCCTGA AGCTCCGCAG GCAGCAGCAG 
ATTGAGCGAG AGCTGAATGG GTACTTGGAG TGGATCTTCA AGGCAGAGGA AGTCATGTTG 
GCAGAGGAGG ACAAGAATGC AGAAGAGAAA TCCCCTTTGG ATGCAGTGTT GAAGAGAGCT 
GCCACCAAGA AGAGCCGAAA TGACCTCATC CATGCAGAAG AGGGGGAGGA CCGGTTTGTA 
GACCTCTGTG CAGTTGGGTC TCCATTTGCT CGTGCCAGCC TCAAGAGTGG GAAGACGGAG 
AGCTCATCGT ACTTCCGGAG AAAGGAGAAG ATGTTCCGGT TCTTTATCCG GCGTATGGTG 
AAAGCACAGA GCTTCTACTG GGTGGTACTG TGTGTGGTGG CCCTGAACAC ACTGTGTGTG 
GCCATGGTGC ACTATAATCA GCCTCAGCGG CTTACCACTG CACTGTACTT TGCAGAGTTT 
GTTTTCCTGG GTCTCTTCCT CACAGAGATG TCCCTGAAGA TGTATGGCCT AGGGCCCAGA 
AGTTACTTCA GGTCTTCCTT CAACTGCTTT GACTTTGGGG TGATTGTGGG GAGTATCTTT 
GAAGTAGTCT GGGCTGCCAT CAAGCCAGGA ACCTCCTTTG GAATCAGTGT GCTGCGGGCT 
CTGCGACTGC TGAGGATATT CAAAGTTACC AAGTATTGGA ACTCTCTGAG GAACCTGGTG 
GTTTCCCTCC TCAATTCCAT GAAGTCCATC ATCAGCCTTC TCTTCCTGCT TTTCCTCTTC 
ATCGTGGTCT TCGCTCTGTT GGGGATGCAG CTGTTTGGGG GACAGTTCAA CTTTCAAGAT 
GAGACTCCAA CCACCAATTT TGATACCTTC CCAGCTGCCA TCCTCACTGT CTTTCAGATC 
CTGACAGGAG AGGATTGGAA TGCCGTAATG TATCATGGGA TTGAGTCGCA AGGTGGAGTC 
AGCAAAGGCA TGTTTTCTTC CTTTTACTTC ATCGTCCTGA CACTGTTTGG AAACTACACC 
CTGCTGAATG TTTTTCTGGC CATTGCTGTG GACAACCTTG CCAATGCCCA GGAGTTGACC 
AAGGATGAAG AGGAGATGGA AGAAGCAGCC AATCAGAAAC TTGCTCTTCA GAAGGCCAAA 
GAAGTAGCTG AAGTCAGCCC CATGTCTGCT GCCAATATCT CCATCGCTGC CAGGCAGCAG 
AACTCGGCCA AGGCGCGCTC AGTATGGGAG CAGCGGGCCA GTCAGCTAAG GCTCCAGAAT 
CTGCGTGCCA GCTGTGAGGC ATTGTACAGT GAGATGGACC CTGAGGAGCG CCTGCGTTAT 
GCCAGCACGC GCCATGTGAG GCCAGACATG AAGACACACA TGGACCGACC CCTAGTGGTG 
GAGCCTGGTC GAGATGGCTT GCGGGGACCC GTTGGGAGCA AGTCAAAGCC TGAAGGCACG 
GAGGCCACAG AAAGCGCGGA CCTACCTCGC AGGCACCACC GGCACCGTGA TAGGGACAAG 
ACCTCAGCCA CAGCACCTGC TGGAGGCGAA CAGGACAGGA CAGAAAGCAC CGAGACCGGG 
GCCCGGGAGG AACGTGCGCG CCCTCGTCGA AGTCACAGCA AGGAGACTCC AGGGGCTGAC 
ACGCAAGTGC GCTGTGAGCG CAGTAGACGT CACCACCGGC GCGGCTCCCC GGAGGAGGCC 
ACTGAACGGG AGCCTCGGCG CCACCGTGCC CACCGGCATG CACAGGACTC AAGCAAGGAG 
GGCACGGCGC CGGTGCTTGT ACCCAAGGGT GAGCGACGAG CAAGACACCG AGGCCCACGC 
ACGGGTCCAC GTGAGGCAGA GAACAACGAG GAGCCCACAC GCAGGCACCG TGCAAGGCAT 
AAGGTGCCAC CCACACTGCA GCCCCCAGAG AGGGAGGCTG CAGAGAAGGA GAGCAACGCG 
GTGGAAGGGG ATAAGGAAAC CCGAAATCAC CAGCCCAAGG AACCTCACTG TGACCTGGAG 
GCCATTGCAG TTACAGGTGT GGGCCCTCTG CACATGCTGC CCAGCACCTG TCTCCAGAAA 
GTGGACGAGC AACCAGAGGA TGCAGACAAC CAGCGTAATG TCACCCGGAT GGGCAGTCAG 
CCCTCAGATC CCAGCACCAC TGTGCATGTC CCAGTGACAC TGACAGGCCC TCCTGGGGAG 
ACCCCTGTAG TTCCCAGTGG TAACATGAAC CTGGAAGGCC AAGCAGAGGG CAAGAAGGAG 
GCAGAGGCGG ATGATGTGCT GAGAAGAGGC CCCAGGCCCA TCGTTCCCTA CAGCTCCATG 
TTTTGTCTCA GCCCCACCAA CCTGCTTCGT CGCTTCTGCC ATTACATTGT GACCATGCGG 
TACTTTGAGA TGGTAATTCT TGTGGTCATT GCCTTGAGCA GCATTGCCCT GGCTGCAGAG 
GATCCTGTGC GGACAGATTC ATTCAGGAAC AACGCTTTAA AGTACATGGA TTACATCTTT 
ACAGGAGTCT TCACCTTTGA AATGGTCATA AAGATGATAG ACTTGGGCTT GCTGCTGCAC 
CCTGGTGCCT ACTTCCGGGA CCTGTGGAAC ATTCTGGACT TCATTGTTGT CAGTGGAGCC 
CTGGTGGCAT TTGCGTTCTC AGGATCCAAA GGGAAAGACA TCAATACCAT CAAGTCTCTG 
AGAGTCCTGC GTGTCCTGAG GCCCCTCAAG ACCATCAAGC GGCTGCCTAA GCTCAAGGCT 
GTCTTTGACT GTGTGGTGAA CTCCCTGAAG AACGTCTTGA ACATCCTGAT TGTCTACATG 
CTCTTCATGT TCATATTTGC CGTCATTGCC GTCCAGCTCT TCAAAGGGAA GTTCTTTTAC 
TGTACTGATG AATCCAAGGA GCTGGAGAGG GACTGCCGGG GTCAGTATTT GGATTATGAG 
AAGGAAGAAG TAGAAGCCCA GCCAAGGCAG TGGAAGAAAT ATGACTTCCA CTATGACAAT 
GTTCTCTGGG CCTTGTTGAC GCTGTTCACA GTGTCCACGG GAGAGGGGTG GCCCATGGTG 
CTGAAACACT CTGTGGATGC CACCTATGAG GAACAGGGGC CCAGTCCCGG GTTCCGGATG 
GAGCTCTCCA TCTTCTACGT GGTCTACTTT GTGGTCTTCC CTTTTTTCTT TGTCAACATC 
TTTGTGGCCT TGATCATTAT CACCTTCCAG GAACAGGGAG ATAAGGTGAT GTCTGAATGC 
AGCTTAGAAA AGAATGAGAG GGCTTGCATT GATTTTGCCA TCAGTGCCAA GCCCCTGACA 
CGGTACATGC CTCAAAACAA ACAGTCGTTC CAGTATAAGA CATGGACATT CGTGGTCTCT 
CCACCCTTTG AGTACTTCAT CATGGCTATG ATAGCCCTCA ACACAGTGGT GCTGATGATG 
AAGTTCTATG ATGCACCTTA TGAGTACGAG CTGATGCTGA AATGCCTGAA CATTGTCTTC 
ACATCCATGT TCTCGATGGA GTGCATACTG AAGATCATCG CCTTTGGGGT ATTGAACTAC 
TTCAGAGATG CCTGGAACGT CTTTGACTTT GTCACGGTTT TGGGAAGTAT TACTGATATT 
TTAGTAACAG AGATAGCGAA CAACTTCATC AACCTAAGCT TCCTTCGCCT CTTCCGGGCG 
GCACGGCTGA TCAAGCTGCT TCGCCAGGGC TACACCATCC GCATCCTATT GTGGACCTTC 
GTCCAGTCCT TTAAGGCGCT GCCCTACGTG TGCCTCCTCA TTGCCATGCT GTTCTTCATC 
TACGCCATCA TCGGCATGCA GGTTTTTGGA AACATTGCCC TTGATGATGA CACCAGTATC 
AACCGACACA ACAACTTCCG GACATTTCTG CAAGCCTTAA TGCTATTGTT CAGGAGTGCC 
ACTGGGGAGG CCTGGCATGA GATCATGCTG TCTTGTCTGG GCAACCGGGC CTGTGACCCA 
CATGCCAACG CCAGTGAGTG CGGGAGCGAC TTTGCCTATT TTTATTTTGT CTCCTTCATC 
TTCCTCTGTT CCTTTCTGAT GTTGAACCTC TTTGTTGCTG TAATCATGGA CAATTTTGAG 
TACCTCACTC GGGACTCTTC CATCCTAGGG CCTCACCACT TAGACGAATT CATTCGAGTC 
TGGGCTGAAT ACGACCCAGC TGCGTGTGGG CGCATCAGTT ACAATGACAT GTTTGAGATG 
CTGAAACACA TGTCCCCACC TCTGGGGTTG GGGAAGAAAT GCCCGGCTCG AGTTGCATAC 
AAGCGCCTGG TTCGCATGAA CATGCCCATA TCCAATGAGG ACATGACGGT GCACTTTACG 
TCCACACTGA TGGCCCTCAT CCGGACAGCA CTGGAGATCA AGCTTGCCCC AGCGGGGACG 
AAGCAGCACC AGTGTGATGC TGAGCTGAGA AAAGAGATCT CTTCTGTGTG GGCTAATCTG 
CCCCAGAAGA CTCTGGACTT ACTGGTACCA CCCCACAAAC CTGACGAGAT GACAGTGGGG 
AAGGTCTATG CTGCTCTCAT GATATTTGAC TTCTACAAAC AGAACAAAAC CACCAGAGAT 
CAGACTCACC AAGCTCCCGG AGGCCTGTCC CAGATGGGTC CCGTTTCCCT GTTCCACCCT 
CTGAAGGCCA CCCTGGAACA GACACAGCCC GCTGTGCTTC GAGGAGCTCG GGTTTTCCTT 
CGGCAAAAGA GTGCAACTTC CCTCAGCAAT GGGGGTGCCA TACAAACCCA GGAAAGTGGC 
ATCAAGGAGT CGCTGTCCTG GGGCACGCAG AGGACCCAAG ATGCACTTTA TGAGGCCAGA 
GCACCTCTAG AACGTGGCCA TTCTGCAGAG ATCCCTGTGG GGCAGTCAGG AACACTGGCT 
GTGGATGTCC AGATGCAGAA CATGACACTG AGAGGACCAG ATGGGGAGCC CCAGCCTGGG 
CTGGAAAGCC AAGGCAGAGC TGCCTCTATG CCACGCCTAG CGGCAGAAAC ACAGCCGGCC 
CCTAATGCCA GCCCCATGAA GCGCTCCATC TCCACACTGG CTCCACGCCC ACATGGGACT 
CAGCTTTGCA GCACAGTTCT GGACCGGCCT CCTCCTAGCC AGGCATCACA TCACCACCAC 
CACCGCTGCC ACCGGCGCAG AGACAAGAAG CAAAGGTCCC TGGAAAAGGG GCCCAGCCTG 
TCTGTTGACC CAGAAGGTGC ACCAAGCACT GCTGCAGGAC CTGGTCTGCC CCATGGAGAA 
GGATCCACCG CCTGCCGGCG GGACCGTAAA CAGGAGCGAG GCCGGTCCCA GGAGCGGAGG 
CAGCCCTCAT CTTCCTCTTC AGAGAAGCAG CGCTTCTATT CCTGTGACCG CTTTGGGAGC 
CGGGAGCCCC CGCAACTGAT GCCCTCACTC AGTAGCCACC CCACATCGCC AACAGCGGCG 
CTAGAGCCAG CACCCCACCC ACAGGGCAGT GGTTCCGTTA ATGGGAGCCC CTTGATGTCA 
ACATCCGGTG CTAGCACCCC GGGCCGAGGT GGGCGGAGGC AGCTCCCCCA GACTCCTCTG 
ACCCCACGCC CCAGCATCAC CTACAAGACC GCCAATTCCT CGCCTGTCCA CTTTGCTGAG 
GGTCAGAGCG GCCTCCCAGC CTTCTCCCCT GGCCGTCTCA GCCGCGGCCT TTCTGAACAC 
AATGCCCTGC TCCAGAAAGA GCCCCTGAGC CAGCCTCTAG CTCCTGGCTC CCGAATTGGC 
TCTGACCCTT ACCTAGGGCA GCGTCTGGAC AGTGAGGCCT CCGCCCACAC CCTGCCTGAG 
GATACACTCA CCTTTGAAGA GGCAGTGGCC ACCAACTCTG GCCGCTCCTC CAGGACTTCC 
TATGTGTCCT CCCTCACTTC CCAATCCCAC CCTCTCCGCC GTGTACCCAA TGGCTATCAC 
TGCACTTTGG GACTCAGCAC TGGCGTCCGG GCGCGGCACA GCTACCACCA CCCCGATCAG 
GACCACTGGT GCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006497696.1
CDS	146..7120
Translation	MVRFGDELGGRYGGTGGGERARGGGAGGAGGPGQGGLPPGQRVLYKQSIAQRARTMALYNPIPVKQNCFTVNRSLFVFSEDNVVRKYAKRITEWPPFEYMILATIIANCIVLALEQHLPDGDKTPMSERLDDTEPYFIGIFCFEAGIKIIALGFVFHKGSYLRNGWNVMDFVVVLTGILATAGTDFDLRTLRAVRVLRPLKLVSGIPSLQVVLKSIMKAMVPLLQIGLLLFFAILMFAIIGLEFYMGKFHKACFPNSTDTEPVGDFPCGKDPPARQCDGDTECREYWPGPNFGITNFDNILFAILTVFQCITMEGWTDILYNTNDAAGNTWNWLYFIPLIIIGSFFMLNLVLGVLSGEFAKERERVENRRAFLKLRRQQQIERELNGYLEWIFKAEEVMLAEEDKNAEEKSPLDAVLKRAATKKSRNDLIHAEEGEDRFVDLCAVGSPFARASLKSGKTESSSYFRRKEKMFRFFIRRMVKAQSFYWVVLCVVALNTLCVAMVHYNQPQRLTTALYFAEFVFLGLFLTEMSLKMYGLGPRSYFRSSFNCFDFGVIVGSIFEVVWAAIKPGTSFGISVLRALRLLRIFKVTKYWNSLRNLVVSLLNSMKSIISLLFLLFLFIVVFALLGMQLFGGQFNFQDETPTTNFDTFPAAILTVFQILTGEDWNAVMYHGIESQGGVSKGMFSSFYFIVLTLFGNYTLLNVFLAIAVDNLANAQELTKDEEEMEEAANQKLALQKAKEVAEVSPMSAANISIAARQQNSAKARSVWEQRASQLRLQNLRASCEALYSEMDPEERLRYASTRHVRPDMKTHMDRPLVVEPGRDGLRGPVGSKSKPEGTEATESADLPRRHHRHRDRDKTSATAPAGGEQDRTESTETGAREERARPRRSHSKETPGADTQVRCERSRRHHRRGSPEEATEREPRRHRAHRHAQDSSKEGTAPVLVPKGERRARHRGPRTGPREAENNEEPTRRHRARHKVPPTLQPPEREAAEKESNAVEGDKETRNHQPKEPHCDLEAIAVTGVGPLHMLPSTCLQKVDEQPEDADNQRNVTRMGSQPSDPSTTVHVPVTLTGPPGETPVVPSGNMNLEGQAEGKKEAEADDVLRRGPRPIVPYSSMFCLSPTNLLRRFCHYIVTMRYFEMVILVVIALSSIALAAEDPVRTDSFRNNALKYMDYIFTGVFTFEMVIKMIDLGLLLHPGAYFRDLWNILDFIVVSGALVAFAFSGSKGKDINTIKSLRVLRVLRPLKTIKRLPKLKAVFDCVVNSLKNVLNILIVYMLFMFIFAVIAVQLFKGKFFYCTDESKELERDCRGQYLDYEKEEVEAQPRQWKKYDFHYDNVLWALLTLFTVSTGEGWPMVLKHSVDATYEEQGPSPGFRMELSIFYVVYFVVFPFFFVNIFVALIIITFQEQGDKVMSECSLEKNERACIDFAISAKPLTRYMPQNKQSFQYKTWTFVVSPPFEYFIMAMIALNTVVLMMKFYDAPYEYELMLKCLNIVFTSMFSMECILKIIAFGVLNYFRDAWNVFDFVTVLGSITDILVTEIANNFINLSFLRLFRAARLIKLLRQGYTIRILLWTFVQSFKALPYVCLLIAMLFFIYAIIGMQVFGNIALDDDTSINRHNNFRTFLQALMLLFRSATGEAWHEIMLSCLGNRACDPHANASECGSDFAYFYFVSFIFLCSFLMLNLFVAVIMDNFEYLTRDSSILGPHHLDEFIRVWAEYDPAACGRISYNDMFEMLKHMSPPLGLGKKCPARVAYKRLVRMNMPISNEDMTVHFTSTLMALIRTALEIKLAPAGTKQHQCDAELRKEISSVWANLPQKTLDLLVPPHKPDEMTVGKVYAALMIFDFYKQNKTTRDQTHQAPGGLSQMGPVSLFHPLKATLEQTQPAVLRGARVFLRQKSATSLSNGGAIQTQESGIKESLSWGTQRTQDALYEARAPLERGHSAEIPVGQSGTLAVDVQMQNMTLRGPDGEPQPGLESQGRAASMPRLAAETQPAPNASPMKRSISTLAPRPHGTQLCSTVLDRPPPSQASHHHHHRCHRRRDKKQRSLEKGPSLSVDPEGAPSTAAGPGLPHGEGSTACRRDRKQERGRSQERRQPSSSSSEKQRFYSCDRFGSREPPQLMPSLSSHPTSPTAALEPAPHPQGSGSVNGSPLMSTSGASTPGRGGRRQLPQTPLTPRPSITYKTANSSPVHFAEGQSGLPAFSPGRLSRGLSEHNALLQKEPLSQPLAPGSRIGSDPYLGQRLDSEASAHTLPEDTLTFEEAVATNSGRSSRTSYVSSLTSQSHPLRRVPNGYHCTLGLSTGVRARHSYHHPDQDHWC

Target ORF information:

RefSeq Version	XM_006497633.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus calcium channel, voltage-dependent, N type, alpha 1B subunit (Cacna1b), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006497633.3

ORF Insert Sequence:

ATGGTCCGCT TCGGGGACGA GCTAGGCGGC CGCTATGGGG GCACCGGCGG CGGGGAGCGG 
GCTCGGGGTG GCGGGGCCGG CGGGGCGGGT GGCCCGGGCC AGGGGGGTCT GCCGCCGGGC 
CAGCGGGTCC TGTACAAGCA GTCCATTGCG CAGCGCGCAC GGACTATGGC CCTGTACAAC 
CCCATCCCAG TCAAGCAGAA CTGCTTCACC GTCAACCGCT CGCTCTTCGT CTTCAGCGAG 
GACAACGTCG TCCGCAAATA CGCTAAGCGC ATCACCGAAT GGCCGCCCTT CGAATACATG 
ATCCTGGCCA CCATCATCGC CAACTGCATT GTTCTGGCCC TGGAGCAGCA CCTCCCTGAT 
GGGGACAAGA CTCCCATGTC TGAGCGACTA GATGACACGG AGCCTTACTT CATCGGGATC 
TTTTGCTTCG AGGCGGGCAT CAAGATCATA GCCCTGGGCT TTGTTTTCCA CAAGGGCTCC 
TACCTTCGGA ACGGCTGGAA TGTCATGGAC TTCGTGGTGG TCCTCACGGG GATTCTCGCC 
ACAGCTGGAA CTGACTTTGA CCTGCGCACA CTGAGGGCTG TGCGTGTGCT TAGGCCCCTG 
AAGCTGGTGT CTGGAATTCC AAGCTTGCAG GTGGTGCTTA AGTCCATCAT GAAGGCCATG 
GTCCCGCTGC TGCAGATTGG GCTGCTGCTC TTCTTTGCCA TCCTCATGTT TGCCATCATC 
GGCCTCGAAT TCTATATGGG CAAATTCCAT AAGGCCTGTT TCCCCAACAG CACAGATACA 
GAGCCTGTGG GTGACTTTCC CTGTGGCAAA GATCCCCCTG CTCGTCAGTG TGATGGTGAC 
ACCGAATGCC GGGAGTACTG GCCAGGACCC AACTTTGGCA TCACCAATTT TGACAACATC 
CTGTTTGCCA TCTTGACAGT GTTCCAGTGT ATCACCATGG AGGGCTGGAC TGACATCCTC 
TACAATACAA ATGATGCGGC TGGCAACACA TGGAACTGGT TGTACTTCAT CCCCCTCATC 
ATCATTGGCT CCTTCTTCAT GCTCAACCTG GTGCTGGGTG TGCTTTCCGG AGAGTTTGCC 
AAGGAGCGGG AGCGAGTCGA GAACCGCCGC GCCTTCCTGA AGCTCCGCAG GCAGCAGCAG 
ATTGAGCGAG AGCTGAATGG GTACTTGGAG TGGATCTTCA AGGCAGAGGA AGTCATGTTG 
GCAGAGGAGG ACAAGAATGC AGAAGAGAAA TCCCCTTTGG ATGCAGTGTT GAAGAGAGCT 
GCCACCAAGA AGAGCCGAAA TGACCTCATC CATGCAGAAG AGGGGGAGGA CCGGTTTGTA 
GACCTCTGTG CAGTTGGGTC TCCATTTGCT CGTGCCAGCC TCAAGAGTGG GAAGACGGAG 
AGCTCATCGT ACTTCCGGAG AAAGGAGAAG ATGTTCCGGT TCTTTATCCG GCGTATGGTG 
AAAGCACAGA GCTTCTACTG GGTGGTACTG TGTGTGGTGG CCCTGAACAC ACTGTGTGTG 
GCCATGGTGC ACTATAATCA GCCTCAGCGG CTTACCACTG CACTGTACTT TGCAGAGTTT 
GTTTTCCTGG GTCTCTTCCT CACAGAGATG TCCCTGAAGA TGTATGGCCT AGGGCCCAGA 
AGTTACTTCA GGTCTTCCTT CAACTGCTTT GACTTTGGGG TGATTGTGGG GAGTATCTTT 
GAAGTAGTCT GGGCTGCCAT CAAGCCAGGA ACCTCCTTTG GAATCAGTGT GCTGCGGGCT 
CTGCGACTGC TGAGGATATT CAAAGTTACC AAGTATTGGA ACTCTCTGAG GAACCTGGTG 
GTTTCCCTCC TCAATTCCAT GAAGTCCATC ATCAGCCTTC TCTTCCTGCT TTTCCTCTTC 
ATCGTGGTCT TCGCTCTGTT GGGGATGCAG CTGTTTGGGG GACAGTTCAA CTTTCAAGAT 
GAGACTCCAA CCACCAATTT TGATACCTTC CCAGCTGCCA TCCTCACTGT CTTTCAGATC 
CTGACAGGAG AGGATTGGAA TGCCGTAATG TATCATGGGA TTGAGTCGCA AGGTGGAGTC 
AGCAAAGGCA TGTTTTCTTC CTTTTACTTC ATCGTCCTGA CACTGTTTGG AAACTACACC 
CTGCTGAATG TTTTTCTGGC CATTGCTGTG GACAACCTTG CCAATGCCCA GGAGTTGACC 
AAGGATGAAG AGGAGATGGA AGAAGCAGCC AATCAGAAAC TTGCTCTTCA GAAGGCCAAA 
GAAGTAGCTG AAGTCAGCCC CATGTCTGCT GCCAATATCT CCATCGCTGC CAGGCAGCAG 
AACTCGGCCA AGGCGCGCTC AGTATGGGAG CAGCGGGCCA GTCAGCTAAG GCTCCAGAAT 
CTGCGTGCCA GCTGTGAGGC ATTGTACAGT GAGATGGACC CTGAGGAGCG CCTGCGTTAT 
GCCAGCACGC GCCATGTGAG GCCAGACATG AAGACACACA TGGACCGACC CCTAGTGGTG 
GAGCCTGGTC GAGATGGCTT GCGGGGACCC GTTGGGAGCA AGTCAAAGCC TGAAGGCACG 
GAGGCCACAG AAAGCGCGGA CCTACCTCGC AGGCACCACC GGCACCGTGA TAGGGACAAG 
ACCTCAGCCA CAGCACCTGC TGGAGGCGAA CAGGACAGGA CAGAAAGCAC CGAGACCGGG 
GCCCGGGAGG AACGTGCGCG CCCTCGTCGA AGTCACAGCA AGGAGACTCC AGGGGCTGAC 
ACGCAAGTGC GCTGTGAGCG CAGTAGACGT CACCACCGGC GCGGCTCCCC GGAGGAGGCC 
ACTGAACGGG AGCCTCGGCG CCACCGTGCC CACCGGCATG CACAGGACTC AAGCAAGGAG 
GGCACGGCGC CGGTGCTTGT ACCCAAGGGT GAGCGACGAG CAAGACACCG AGGCCCACGC 
ACGGGTCCAC GTGAGGCAGA GAACAACGAG GAGCCCACAC GCAGGCACCG TGCAAGGCAT 
AAGGTGCCAC CCACACTGCA GCCCCCAGAG AGGGAGGCTG CAGAGAAGGA GAGCAACGCG 
GTGGAAGGGG ATAAGGAAAC CCGAAATCAC CAGCCCAAGG AACCTCACTG TGACCTGGAG 
GCCATTGCAG TTACAGGTGT GGGCCCTCTG CACATGCTGC CCAGCACCTG TCTCCAGAAA 
GTGGACGAGC AACCAGAGGA TGCAGACAAC CAGCGTAATG TCACCCGGAT GGGCAGTCAG 
CCCTCAGATC CCAGCACCAC TGTGCATGTC CCAGTGACAC TGACAGGCCC TCCTGGGGAG 
ACCCCTGTAG TTCCCAGTGG TAACATGAAC CTGGAAGGCC AAGCAGAGGG CAAGAAGGAG 
GCAGAGGCGG ATGATGTGCT GAGAAGAGGC CCCAGGCCCA TCGTTCCCTA CAGCTCCATG 
TTTTGTCTCA GCCCCACCAA CCTGCTTCGT CGCTTCTGCC ATTACATTGT GACCATGCGG 
TACTTTGAGA TGGTAATTCT TGTGGTCATT GCCTTGAGCA GCATTGCCCT GGCTGCAGAG 
GATCCTGTGC GGACAGATTC ATTCAGGAAC AACGCTTTAA AGTACATGGA TTACATCTTT 
ACAGGAGTCT TCACCTTTGA AATGGTCATA AAGATGATAG ACTTGGGCTT GCTGCTGCAC 
CCTGGTGCCT ACTTCCGGGA CCTGTGGAAC ATTCTGGACT TCATTGTTGT CAGTGGAGCC 
CTGGTGGCAT TTGCGTTCTC AGGATCCAAA GGGAAAGACA TCAATACCAT CAAGTCTCTG 
AGAGTCCTGC GTGTCCTGAG GCCCCTCAAG ACCATCAAGC GGCTGCCTAA GCTCAAGGCT 
GTCTTTGACT GTGTGGTGAA CTCCCTGAAG AACGTCTTGA ACATCCTGAT TGTCTACATG 
CTCTTCATGT TCATATTTGC CGTCATTGCC GTCCAGCTCT TCAAAGGGAA GTTCTTTTAC 
TGTACTGATG AATCCAAGGA GCTGGAGAGG GACTGCCGGG GTCAGTATTT GGATTATGAG 
AAGGAAGAAG TAGAAGCCCA GCCAAGGCAG TGGAAGAAAT ATGACTTCCA CTATGACAAT 
GTTCTCTGGG CCTTGTTGAC GCTGTTCACA GTGTCCACGG GAGAGGGGTG GCCCATGGTG 
CTGAAACACT CTGTGGATGC CACCTATGAG GAACAGGGGC CCAGTCCCGG GTTCCGGATG 
GAGCTCTCCA TCTTCTACGT GGTCTACTTT GTGGTCTTCC CTTTTTTCTT TGTCAACATC 
TTTGTGGCCT TGATCATTAT CACCTTCCAG GAACAGGGAG ATAAGGTGAT GTCTGAATGC 
AGCTTAGAAA AGAATGAGAG GGCTTGCATT GATTTTGCCA TCAGTGCCAA GCCCCTGACA 
CGGTACATGC CTCAAAACAA ACAGTCGTTC CAGTATAAGA CATGGACATT CGTGGTCTCT 
CCACCCTTTG AGTACTTCAT CATGGCTATG ATAGCCCTCA ACACAGTGGT GCTGATGATG 
AAGTTCTATG ATGCACCTTA TGAGTACGAG CTGATGCTGA AATGCCTGAA CATTGTCTTC 
ACATCCATGT TCTCGATGGA GTGCATACTG AAGATCATCG CCTTTGGGGT ATTGAACTAC 
TTCAGAGATG CCTGGAACGT CTTTGACTTT GTCACGGTTT TGGGAAGTAT TACTGATATT 
TTAGTAACAG AGATAGCGAA CAACTTCATC AACCTAAGCT TCCTTCGCCT CTTCCGGGCG 
GCACGGCTGA TCAAGCTGCT TCGCCAGGGC TACACCATCC GCATCCTATT GTGGACCTTC 
GTCCAGTCCT TTAAGGCGCT GCCCTACGTG TGCCTCCTCA TTGCCATGCT GTTCTTCATC 
TACGCCATCA TCGGCATGCA GGTTTTTGGA AACATTGCCC TTGATGATGA CACCAGTATC 
AACCGACACA ACAACTTCCG GACATTTCTG CAAGCCTTAA TGCTATTGTT CAGGAGTGCC 
ACTGGGGAGG CCTGGCATGA GATCATGCTG TCTTGTCTGG GCAACCGGGC CTGTGACCCA 
CATGCCAACG CCAGTGAGTG CGGGAGCGAC TTTGCCTATT TTTATTTTGT CTCCTTCATC 
TTCCTCTGTT CCTTTCTGAT GTTGAACCTC TTTGTTGCTG TAATCATGGA CAATTTTGAG 
TACCTCACTC GGGACTCTTC CATCCTAGGG CCTCACCACT TAGACGAATT CATTCGAGTC 
TGGGCTGAAT ACGACCCAGC TGCGTGTGGG CGCATCAGTT ACAATGACAT GTTTGAGATG 
CTGAAACACA TGTCCCCACC TCTGGGGTTG GGGAAGAAAT GCCCGGCTCG AGTTGCATAC 
AAGCGCCTGG TTCGCATGAA CATGCCCATA TCCAATGAGG ACATGACGGT GCACTTTACG 
TCCACACTGA TGGCCCTCAT CCGGACAGCA CTGGAGATCA AGCTTGCCCC AGCGGGGACG 
AAGCAGCACC AGTGTGATGC TGAGCTGAGA AAAGAGATCT CTTCTGTGTG GGCTAATCTG 
CCCCAGAAGA CTCTGGACTT ACTGGTACCA CCCCACAAAC CTGACGAGAT GACAGTGGGG 
AAGGTCTATG CTGCTCTCAT GATATTTGAC TTCTACAAAC AGAACAAAAC CACCAGAGAT 
CAGACTCACC AAGCTCCCGG AGGCCTGTCC CAGATGGGTC CCGTTTCCCT GTTCCACCCT 
CTGAAGGCCA CCCTGGAACA GACACAGCCC GCTGTGCTTC GAGGAGCTCG GGTTTTCCTT 
CGGCAAAAGA GTGCAACTTC CCTCAGCAAT GGGGGTGCCA TACAAACCCA GGAAAGTGGC 
ATCAAGGAGT CGCTGTCCTG GGGCACGCAG AGGACCCAAG ATGCACTTTA TGAGGCCAGA 
GCACCTCTAG AACGTGGCCA TTCTGCAGAG ATCCCTGTGG GGCAGTCAGG AACACTGGCT 
GTGGATGTCC AGATGCAGAA CATGACACTG AGAGGACCAG ATGGGGAGCC CCAGCCTGGG 
CTGGAAAGCC AAGGCAGAGC TGCCTCTATG CCACGCCTAG CGGCAGAAAC ACAGCCGGCC 
CCTAATGCCA GCCCCATGAA GCGCTCCATC TCCACACTGG CTCCACGCCC ACATGGGACT 
CAGCTTTGCA GCACAGTTCT GGACCGGCCT CCTCCTAGCC AGGCATCACA TCACCACCAC 
CACCGCTGCC ACCGGCGCAG AGACAAGAAG CAAAGGTCCC TGGAAAAGGG GCCCAGCCTG 
TCTGTTGACC CAGAAGGTGC ACCAAGCACT GCTGCAGGAC CTGGTCTGCC CCATGGAGAA 
GGATCCACCG CCTGCCGGCG GGACCGTAAA CAGGAGCGAG GCCGGTCCCA GGAGCGGAGG 
CAGCCCTCAT CTTCCTCTTC AGAGAAGCAG CGCTTCTATT CCTGTGACCG CTTTGGGAGC 
CGGGAGCCCC CGCAACTGAT GCCCTCACTC AGTAGCCACC CCACATCGCC AACAGCGGCG 
CTAGAGCCAG CACCCCACCC ACAGGGCAGT GGTTCCGTTA ATGGGAGCCC CTTGATGTCA 
ACATCCGGTG CTAGCACCCC GGGCCGAGGT GGGCGGAGGC AGCTCCCCCA GACTCCTCTG 
ACCCCACGCC CCAGCATCAC CTACAAGACC GCCAATTCCT CGCCTGTCCA CTTTGCTGAG 
GGTCAGAGCG GCCTCCCAGC CTTCTCCCCT GGCCGTCTCA GCCGCGGCCT TTCTGAACAC 
AATGCCCTGC TCCAGAAAGA GCCCCTGAGC CAGCCTCTAG CTCCTGGCTC CCGAATTGGC 
TCTGACCCTT ACCTAGGGCA GCGTCTGGAC AGTGAGGCCT CCGCCCACAC CCTGCCTGAG 
GATACACTCA CCTTTGAAGA GGCAGTGGCC ACCAACTCTG GCCGCTCCTC CAGGACTTCC 
TATGTGTCCT CCCTCACTTC CCAATCCCAC CCTCTCCGCC GTGTACCCAA TGGCTATCAC 
TGCACTTTGG GACTCAGCAC TGGCGTCCGG GCGCGGCACA GCTACCACCA CCCCGATCAG 
GACCACTGGT GCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cacna1b ( XM_006497634.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006497697.1 Mus musculus calcium channel, voltage-dependent, N type, alpha 1B subunit (Cacna1b), transcript variant X12, mRNA.

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CloneID	OMu29265
Clone ID Related Accession (Same CDS sequence)	XM_006497634.3
Accession Version	XM_006497634.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6975bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	voltage-dependent N-type calcium channel subunit alpha-1B isoform X12
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006497634.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTCCGCT TCGGGGACGA GCTAGGCGGC CGCTATGGGG GCACCGGCGG CGGGGAGCGG 
GCTCGGGGTG GCGGGGCCGG CGGGGCGGGT GGCCCGGGCC AGGGGGGTCT GCCGCCGGGC 
CAGCGGGTCC TGTACAAGCA GTCCATTGCG CAGCGCGCAC GGACTATGGC CCTGTACAAC 
CCCATCCCAG TCAAGCAGAA CTGCTTCACC GTCAACCGCT CGCTCTTCGT CTTCAGCGAG 
GACAACGTCG TCCGCAAATA CGCTAAGCGC ATCACCGAAT GGCCGCCCTT CGAATACATG 
ATCCTGGCCA CCATCATCGC CAACTGCATT GTTCTGGCCC TGGAGCAGCA CCTCCCTGAT 
GGGGACAAGA CTCCCATGTC TGAGCGACTA GATGACACGG AGCCTTACTT CATCGGGATC 
TTTTGCTTCG AGGCGGGCAT CAAGATCATA GCCCTGGGCT TTGTTTTCCA CAAGGGCTCC 
TACCTTCGGA ACGGCTGGAA TGTCATGGAC TTCGTGGTGG TCCTCACGGG GATTCTCGCC 
ACAGCTGGAA CTGACTTTGA CCTGCGCACA CTGAGGGCTG TGCGTGTGCT TAGGCCCCTG 
AAGCTGGTGT CTGGAATTCC AAGCTTGCAG GTGGTGCTTA AGTCCATCAT GAAGGCCATG 
GTCCCGCTGC TGCAGATTGG GCTGCTGCTC TTCTTTGCCA TCCTCATGTT TGCCATCATC 
GGCCTCGAAT TCTATATGGG CAAATTCCAT AAGGCCTGTT TCCCCAACAG CACAGATACA 
GAGCCTGTGG GTGACTTTCC CTGTGGCAAA GATCCCCCTG CTCGTCAGTG TGATGGTGAC 
ACCGAATGCC GGGAGTACTG GCCAGGACCC AACTTTGGCA TCACCAATTT TGACAACATC 
CTGTTTGCCA TCTTGACAGT GTTCCAGTGT ATCACCATGG AGGGCTGGAC TGACATCCTC 
TACAATACAA ATGATGCGGC TGGCAACACA TGGAACTGGT TGTACTTCAT CCCCCTCATC 
ATCATTGGCT CCTTCTTCAT GCTCAACCTG GTGCTGGGTG TGCTTTCCGG AGAGTTTGCC 
AAGGAGCGGG AGCGAGTCGA GAACCGCCGC GCCTTCCTGA AGCTCCGCAG GCAGCAGCAG 
ATTGAGCGAG AGCTGAATGG GTACTTGGAG TGGATCTTCA AGGCAGAGGA AGTCATGTTG 
GCAGAGGAGG ACAAGAATGC AGAAGAGAAA TCCCCTTTGG ATGTGTTGAA GAGAGCTGCC 
ACCAAGAAGA GCCGAAATGA CCTCATCCAT GCAGAAGAGG GGGAGGACCG GTTTGTAGAC 
CTCTGTGCAG TTGGGTCTCC ATTTGCTCGT GCCAGCCTCA AGAGTGGGAA GACGGAGAGC 
TCATCGTACT TCCGGAGAAA GGAGAAGATG TTCCGGTTCT TTATCCGGCG TATGGTGAAA 
GCACAGAGCT TCTACTGGGT GGTACTGTGT GTGGTGGCCC TGAACACACT GTGTGTGGCC 
ATGGTGCACT ATAATCAGCC TCAGCGGCTT ACCACTGCAC TGTACTTTGC AGAGTTTGTT 
TTCCTGGGTC TCTTCCTCAC AGAGATGTCC CTGAAGATGT ATGGCCTAGG GCCCAGAAGT 
TACTTCAGGT CTTCCTTCAA CTGCTTTGAC TTTGGGGTGA TTGTGGGGAG TATCTTTGAA 
GTAGTCTGGG CTGCCATCAA GCCAGGAACC TCCTTTGGAA TCAGTGTGCT GCGGGCTCTG 
CGACTGCTGA GGATATTCAA AGTTACCAAG TATTGGAACT CTCTGAGGAA CCTGGTGGTT 
TCCCTCCTCA ATTCCATGAA GTCCATCATC AGCCTTCTCT TCCTGCTTTT CCTCTTCATC 
GTGGTCTTCG CTCTGTTGGG GATGCAGCTG TTTGGGGGAC AGTTCAACTT TCAAGATGAG 
ACTCCAACCA CCAATTTTGA TACCTTCCCA GCTGCCATCC TCACTGTCTT TCAGATCCTG 
ACAGGAGAGG ATTGGAATGC CGTAATGTAT CATGGGATTG AGTCGCAAGG TGGAGTCAGC 
AAAGGCATGT TTTCTTCCTT TTACTTCATC GTCCTGACAC TGTTTGGAAA CTACACCCTG 
CTGAATGTTT TTCTGGCCAT TGCTGTGGAC AACCTTGCCA ATGCCCAGGA GTTGACCAAG 
GATGAAGAGG AGATGGAAGA AGCAGCCAAT CAGAAACTTG CTCTTCAGAA GGCCAAAGAA 
GTAGCTGAAG TCAGCCCCAT GTCTGCTGCC AATATCTCCA TCGCTGCGCA GCAGAACTCG 
GCCAAGGCGC GCTCAGTATG GGAGCAGCGG GCCAGTCAGC TAAGGCTCCA GAATCTGCGT 
GCCAGCTGTG AGGCATTGTA CAGTGAGATG GACCCTGAGG AGCGCCTGCG TTATGCCAGC 
ACGCGCCATG TGAGGCCAGA CATGAAGACA CACATGGACC GACCCCTAGT GGTGGAGCCT 
GGTCGAGATG GCTTGCGGGG ACCCGTTGGG AGCAAGTCAA AGCCTGAAGG CACGGAGGCC 
ACAGAAAGCG CGGACCTACC TCGCAGGCAC CACCGGCACC GTGATAGGGA CAAGACCTCA 
GCCACAGCAC CTGCTGGAGG CGAACAGGAC AGGACAGAAA GCACCGAGAC CGGGGCCCGG 
GAGGAACGTG CGCGCCCTCG TCGAAGTCAC AGCAAGGAGA CTCCAGGGGC TGACACGCAA 
GTGCGCTGTG AGCGCAGTAG ACGTCACCAC CGGCGCGGCT CCCCGGAGGA GGCCACTGAA 
CGGGAGCCTC GGCGCCACCG TGCCCACCGG CATGCACAGG ACTCAAGCAA GGAGGGCACG 
GCGCCGGTGC TTGTACCCAA GGGTGAGCGA CGAGCAAGAC ACCGAGGCCC ACGCACGGGT 
CCACGTGAGG CAGAGAACAA CGAGGAGCCC ACACGCAGGC ACCGTGCAAG GCATAAGGTG 
CCACCCACAC TGCAGCCCCC AGAGAGGGAG GCTGCAGAGA AGGAGAGCAA CGCGGTGGAA 
GGGGATAAGG AAACCCGAAA TCACCAGCCC AAGGAACCTC ACTGTGACCT GGAGGCCATT 
GCAGTTACAG GTGTGGGCCC TCTGCACATG CTGCCCAGCA CCTGTCTCCA GAAAGTGGAC 
GAGCAACCAG AGGATGCAGA CAACCAGCGT AATGTCACCC GGATGGGCAG TCAGCCCTCA 
GATCCCAGCA CCACTGTGCA TGTCCCAGTG ACACTGACAG GCCCTCCTGG GGAGACCCCT 
GTAGTTCCCA GTGGTAACAT GAACCTGGAA GGCCAAGCAG AGGGCAAGAA GGAGGCAGAG 
GCGGATGATG TGCTGAGAAG AGGCCCCAGG CCCATCGTTC CCTACAGCTC CATGTTTTGT 
CTCAGCCCCA CCAACCTGCT TCGTCGCTTC TGCCATTACA TTGTGACCAT GCGGTACTTT 
GAGATGGTAA TTCTTGTGGT CATTGCCTTG AGCAGCATTG CCCTGGCTGC AGAGGATCCT 
GTGCGGACAG ATTCATTCAG GAACAACGCT TTAAAGTACA TGGATTACAT CTTTACAGGA 
GTCTTCACCT TTGAAATGGT CATAAAGATG ATAGACTTGG GCTTGCTGCT GCACCCTGGT 
GCCTACTTCC GGGACCTGTG GAACATTCTG GACTTCATTG TTGTCAGTGG AGCCCTGGTG 
GCATTTGCGT TCTCAGGATC CAAAGGGAAA GACATCAATA CCATCAAGTC TCTGAGAGTC 
CTGCGTGTCC TGAGGCCCCT CAAGACCATC AAGCGGCTGC CTAAGCTCAA GGCTGTCTTT 
GACTGTGTGG TGAACTCCCT GAAGAACGTC TTGAACATCC TGATTGTCTA CATGCTCTTC 
ATGTTCATAT TTGCCGTCAT TGCCGTCCAG CTCTTCAAAG GGAAGTTCTT TTACTGTACT 
GATGAATCCA AGGAGCTGGA GAGGGACTGC CGGGGTCAGT ATTTGGATTA TGAGAAGGAA 
GAAGTAGAAG CCCAGCCAAG GCAGTGGAAG AAATATGACT TCCACTATGA CAATGTTCTC 
TGGGCCTTGT TGACGCTGTT CACAGTGTCC ACGGGAGAGG GGTGGCCCAT GGTGCTGAAA 
CACTCTGTGG ATGCCACCTA TGAGGAACAG GGGCCCAGTC CCGGGTTCCG GATGGAGCTC 
TCCATCTTCT ACGTGGTCTA CTTTGTGGTC TTCCCTTTTT TCTTTGTCAA CATCTTTGTG 
GCCTTGATCA TTATCACCTT CCAGGAACAG GGAGATAAGG TGATGTCTGA ATGCAGCTTA 
GAAAAGAATG AGAGGGCTTG CATTGATTTT GCCATCAGTG CCAAGCCCCT GACACGGTAC 
ATGCCTCAAA ACAAACAGTC GTTCCAGTAT AAGACATGGA CATTCGTGGT CTCTCCACCC 
TTTGAGTACT TCATCATGGC TATGATAGCC CTCAACACAG TGGTGCTGAT GATGAAGTTC 
TATGATGCAC CTTATGAGTA CGAGCTGATG CTGAAATGCC TGAACATTGT CTTCACATCC 
ATGTTCTCGA TGGAGTGCAT ACTGAAGATC ATCGCCTTTG GGGTATTGAA CTACTTCAGA 
GATGCCTGGA ACGTCTTTGA CTTTGTCACG GTTTTGGGAA GTATTACTGA TATTTTAGTA 
ACAGAGATAG CGGAAACGAA CAACTTCATC AACCTAAGCT TCCTTCGCCT CTTCCGGGCG 
GCACGGCTGA TCAAGCTGCT TCGCCAGGGC TACACCATCC GCATCCTATT GTGGACCTTC 
GTCCAGTCCT TTAAGGCGCT GCCCTACGTG TGCCTCCTCA TTGCCATGCT GTTCTTCATC 
TACGCCATCA TCGGCATGCA GGTTTTTGGA AACATTGCCC TTGATGATGA CACCAGTATC 
AACCGACACA ACAACTTCCG GACATTTCTG CAAGCCTTAA TGCTATTGTT CAGGAGTGCC 
ACTGGGGAGG CCTGGCATGA GATCATGCTG TCTTGTCTGG GCAACCGGGC CTGTGACCCA 
CATGCCAACG CCAGTGAGTG CGGGAGCGAC TTTGCCTATT TTTATTTTGT CTCCTTCATC 
TTCCTCTGTT CCTTTCTGAT GTTGAACCTC TTTGTTGCTG TAATCATGGA CAATTTTGAG 
TACCTCACTC GGGACTCTTC CATCCTAGGG CCTCACCACT TAGACGAATT CATTCGAGTC 
TGGGCTGAAT ACGACCCAGC TGCGTGTGGG CGCATCAGTT ACAATGACAT GTTTGAGATG 
CTGAAACACA TGTCCCCACC TCTGGGGTTG GGGAAGAAAT GCCCGGCTCG AGTTGCATAC 
AAGCGCCTGG TTCGCATGAA CATGCCCATA TCCAATGAGG ACATGACGGT GCACTTTACG 
TCCACACTGA TGGCCCTCAT CCGGACAGCA CTGGAGATCA AGCTTGCCCC AGCGGGGACG 
AAGCAGCACC AGTGTGATGC TGAGCTGAGA AAAGAGATCT CTTCTGTGTG GGCTAATCTG 
CCCCAGAAGA CTCTGGACTT ACTGGTACCA CCCCACAAAC CTGACGAGAT GACAGTGGGG 
AAGGTCTATG CTGCTCTCAT GATATTTGAC TTCTACAAAC AGAACAAAAC CACCAGAGAT 
CAGACTCACC AAGCTCCCGG AGGCCTGTCC CAGATGGGTC CCGTTTCCCT GTTCCACCCT 
CTGAAGGCCA CCCTGGAACA GACACAGCCC GCTGTGCTTC GAGGAGCTCG GGTTTTCCTT 
CGGCAAAAGA GTGCAACTTC CCTCAGCAAT GGGGGTGCCA TACAAACCCA GGAAAGTGGC 
ATCAAGGAGT CGCTGTCCTG GGGCACGCAG AGGACCCAAG ATGCACTTTA TGAGGCCAGA 
GCACCTCTAG AACGTGGCCA TTCTGCAGAG ATCCCTGTGG GGCAGTCAGG AACACTGGCT 
GTGGATGTCC AGATGCAGAA CATGACACTG AGAGGACCAG ATGGGGAGCC CCAGCCTGGG 
CTGGAAAGCC AAGGCAGAGC TGCCTCTATG CCACGCCTAG CGGCAGAAAC ACAGCCGGCC 
CCTAATGCCA GCCCCATGAA GCGCTCCATC TCCACACTGG CTCCACGCCC ACATGGGACT 
CAGCTTTGCA GCACAGTTCT GGACCGGCCT CCTCCTAGCC AGGCATCACA TCACCACCAC 
CACCGCTGCC ACCGGCGCAG AGACAAGAAG CAAAGGTCCC TGGAAAAGGG GCCCAGCCTG 
TCTGTTGACC CAGAAGGTGC ACCAAGCACT GCTGCAGGAC CTGGTCTGCC CCATGGAGAA 
GGATCCACCG CCTGCCGGCG GGACCGTAAA CAGGAGCGAG GCCGGTCCCA GGAGCGGAGG 
CAGCCCTCAT CTTCCTCTTC AGAGAAGCAG CGCTTCTATT CCTGTGACCG CTTTGGGAGC 
CGGGAGCCCC CGCAACTGAT GCCCTCACTC AGTAGCCACC CCACATCGCC AACAGCGGCG 
CTAGAGCCAG CACCCCACCC ACAGGGCAGT GGTTCCGTTA ATGGGAGCCC CTTGATGTCA 
ACATCCGGTG CTAGCACCCC GGGCCGAGGT GGGCGGAGGC AGCTCCCCCA GACTCCTCTG 
ACCCCACGCC CCAGCATCAC CTACAAGACC GCCAATTCCT CGCCTGTCCA CTTTGCTGAG 
GGTCAGAGCG GCCTCCCAGC CTTCTCCCCT GGCCGTCTCA GCCGCGGCCT TTCTGAACAC 
AATGCCCTGC TCCAGAAAGA GCCCCTGAGC CAGCCTCTAG CTCCTGGCTC CCGAATTGGC 
TCTGACCCTT ACCTAGGGCA GCGTCTGGAC AGTGAGGCCT CCGCCCACAC CCTGCCTGAG 
GATACACTCA CCTTTGAAGA GGCAGTGGCC ACCAACTCTG GCCGCTCCTC CAGGACTTCC 
TATGTGTCCT CCCTCACTTC CCAATCCCAC CCTCTCCGCC GTGTACCCAA TGGCTATCAC 
TGCACTTTGG GACTCAGCAC TGGCGTCCGG GCGCGGCACA GCTACCACCA CCCCGATCAG 
GACCACTGGT GCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006497697.1
CDS	146..7120
Translation	MVRFGDELGGRYGGTGGGERARGGGAGGAGGPGQGGLPPGQRVLYKQSIAQRARTMALYNPIPVKQNCFTVNRSLFVFSEDNVVRKYAKRITEWPPFEYMILATIIANCIVLALEQHLPDGDKTPMSERLDDTEPYFIGIFCFEAGIKIIALGFVFHKGSYLRNGWNVMDFVVVLTGILATAGTDFDLRTLRAVRVLRPLKLVSGIPSLQVVLKSIMKAMVPLLQIGLLLFFAILMFAIIGLEFYMGKFHKACFPNSTDTEPVGDFPCGKDPPARQCDGDTECREYWPGPNFGITNFDNILFAILTVFQCITMEGWTDILYNTNDAAGNTWNWLYFIPLIIIGSFFMLNLVLGVLSGEFAKERERVENRRAFLKLRRQQQIERELNGYLEWIFKAEEVMLAEEDKNAEEKSPLDVLKRAATKKSRNDLIHAEEGEDRFVDLCAVGSPFARASLKSGKTESSSYFRRKEKMFRFFIRRMVKAQSFYWVVLCVVALNTLCVAMVHYNQPQRLTTALYFAEFVFLGLFLTEMSLKMYGLGPRSYFRSSFNCFDFGVIVGSIFEVVWAAIKPGTSFGISVLRALRLLRIFKVTKYWNSLRNLVVSLLNSMKSIISLLFLLFLFIVVFALLGMQLFGGQFNFQDETPTTNFDTFPAAILTVFQILTGEDWNAVMYHGIESQGGVSKGMFSSFYFIVLTLFGNYTLLNVFLAIAVDNLANAQELTKDEEEMEEAANQKLALQKAKEVAEVSPMSAANISIAAQQNSAKARSVWEQRASQLRLQNLRASCEALYSEMDPEERLRYASTRHVRPDMKTHMDRPLVVEPGRDGLRGPVGSKSKPEGTEATESADLPRRHHRHRDRDKTSATAPAGGEQDRTESTETGAREERARPRRSHSKETPGADTQVRCERSRRHHRRGSPEEATEREPRRHRAHRHAQDSSKEGTAPVLVPKGERRARHRGPRTGPREAENNEEPTRRHRARHKVPPTLQPPEREAAEKESNAVEGDKETRNHQPKEPHCDLEAIAVTGVGPLHMLPSTCLQKVDEQPEDADNQRNVTRMGSQPSDPSTTVHVPVTLTGPPGETPVVPSGNMNLEGQAEGKKEAEADDVLRRGPRPIVPYSSMFCLSPTNLLRRFCHYIVTMRYFEMVILVVIALSSIALAAEDPVRTDSFRNNALKYMDYIFTGVFTFEMVIKMIDLGLLLHPGAYFRDLWNILDFIVVSGALVAFAFSGSKGKDINTIKSLRVLRVLRPLKTIKRLPKLKAVFDCVVNSLKNVLNILIVYMLFMFIFAVIAVQLFKGKFFYCTDESKELERDCRGQYLDYEKEEVEAQPRQWKKYDFHYDNVLWALLTLFTVSTGEGWPMVLKHSVDATYEEQGPSPGFRMELSIFYVVYFVVFPFFFVNIFVALIIITFQEQGDKVMSECSLEKNERACIDFAISAKPLTRYMPQNKQSFQYKTWTFVVSPPFEYFIMAMIALNTVVLMMKFYDAPYEYELMLKCLNIVFTSMFSMECILKIIAFGVLNYFRDAWNVFDFVTVLGSITDILVTEIAETNNFINLSFLRLFRAARLIKLLRQGYTIRILLWTFVQSFKALPYVCLLIAMLFFIYAIIGMQVFGNIALDDDTSINRHNNFRTFLQALMLLFRSATGEAWHEIMLSCLGNRACDPHANASECGSDFAYFYFVSFIFLCSFLMLNLFVAVIMDNFEYLTRDSSILGPHHLDEFIRVWAEYDPAACGRISYNDMFEMLKHMSPPLGLGKKCPARVAYKRLVRMNMPISNEDMTVHFTSTLMALIRTALEIKLAPAGTKQHQCDAELRKEISSVWANLPQKTLDLLVPPHKPDEMTVGKVYAALMIFDFYKQNKTTRDQTHQAPGGLSQMGPVSLFHPLKATLEQTQPAVLRGARVFLRQKSATSLSNGGAIQTQESGIKESLSWGTQRTQDALYEARAPLERGHSAEIPVGQSGTLAVDVQMQNMTLRGPDGEPQPGLESQGRAASMPRLAAETQPAPNASPMKRSISTLAPRPHGTQLCSTVLDRPPPSQASHHHHHRCHRRRDKKQRSLEKGPSLSVDPEGAPSTAAGPGLPHGEGSTACRRDRKQERGRSQERRQPSSSSSEKQRFYSCDRFGSREPPQLMPSLSSHPTSPTAALEPAPHPQGSGSVNGSPLMSTSGASTPGRGGRRQLPQTPLTPRPSITYKTANSSPVHFAEGQSGLPAFSPGRLSRGLSEHNALLQKEPLSQPLAPGSRIGSDPYLGQRLDSEASAHTLPEDTLTFEEAVATNSGRSSRTSYVSSLTSQSHPLRRVPNGYHCTLGLSTGVRARHSYHHPDQDHWC

Target ORF information:

RefSeq Version	XM_006497634.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus calcium channel, voltage-dependent, N type, alpha 1B subunit (Cacna1b), transcript variant X12, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006497634.3

ORF Insert Sequence:

ATGGTCCGCT TCGGGGACGA GCTAGGCGGC CGCTATGGGG GCACCGGCGG CGGGGAGCGG 
GCTCGGGGTG GCGGGGCCGG CGGGGCGGGT GGCCCGGGCC AGGGGGGTCT GCCGCCGGGC 
CAGCGGGTCC TGTACAAGCA GTCCATTGCG CAGCGCGCAC GGACTATGGC CCTGTACAAC 
CCCATCCCAG TCAAGCAGAA CTGCTTCACC GTCAACCGCT CGCTCTTCGT CTTCAGCGAG 
GACAACGTCG TCCGCAAATA CGCTAAGCGC ATCACCGAAT GGCCGCCCTT CGAATACATG 
ATCCTGGCCA CCATCATCGC CAACTGCATT GTTCTGGCCC TGGAGCAGCA CCTCCCTGAT 
GGGGACAAGA CTCCCATGTC TGAGCGACTA GATGACACGG AGCCTTACTT CATCGGGATC 
TTTTGCTTCG AGGCGGGCAT CAAGATCATA GCCCTGGGCT TTGTTTTCCA CAAGGGCTCC 
TACCTTCGGA ACGGCTGGAA TGTCATGGAC TTCGTGGTGG TCCTCACGGG GATTCTCGCC 
ACAGCTGGAA CTGACTTTGA CCTGCGCACA CTGAGGGCTG TGCGTGTGCT TAGGCCCCTG 
AAGCTGGTGT CTGGAATTCC AAGCTTGCAG GTGGTGCTTA AGTCCATCAT GAAGGCCATG 
GTCCCGCTGC TGCAGATTGG GCTGCTGCTC TTCTTTGCCA TCCTCATGTT TGCCATCATC 
GGCCTCGAAT TCTATATGGG CAAATTCCAT AAGGCCTGTT TCCCCAACAG CACAGATACA 
GAGCCTGTGG GTGACTTTCC CTGTGGCAAA GATCCCCCTG CTCGTCAGTG TGATGGTGAC 
ACCGAATGCC GGGAGTACTG GCCAGGACCC AACTTTGGCA TCACCAATTT TGACAACATC 
CTGTTTGCCA TCTTGACAGT GTTCCAGTGT ATCACCATGG AGGGCTGGAC TGACATCCTC 
TACAATACAA ATGATGCGGC TGGCAACACA TGGAACTGGT TGTACTTCAT CCCCCTCATC 
ATCATTGGCT CCTTCTTCAT GCTCAACCTG GTGCTGGGTG TGCTTTCCGG AGAGTTTGCC 
AAGGAGCGGG AGCGAGTCGA GAACCGCCGC GCCTTCCTGA AGCTCCGCAG GCAGCAGCAG 
ATTGAGCGAG AGCTGAATGG GTACTTGGAG TGGATCTTCA AGGCAGAGGA AGTCATGTTG 
GCAGAGGAGG ACAAGAATGC AGAAGAGAAA TCCCCTTTGG ATGTGTTGAA GAGAGCTGCC 
ACCAAGAAGA GCCGAAATGA CCTCATCCAT GCAGAAGAGG GGGAGGACCG GTTTGTAGAC 
CTCTGTGCAG TTGGGTCTCC ATTTGCTCGT GCCAGCCTCA AGAGTGGGAA GACGGAGAGC 
TCATCGTACT TCCGGAGAAA GGAGAAGATG TTCCGGTTCT TTATCCGGCG TATGGTGAAA 
GCACAGAGCT TCTACTGGGT GGTACTGTGT GTGGTGGCCC TGAACACACT GTGTGTGGCC 
ATGGTGCACT ATAATCAGCC TCAGCGGCTT ACCACTGCAC TGTACTTTGC AGAGTTTGTT 
TTCCTGGGTC TCTTCCTCAC AGAGATGTCC CTGAAGATGT ATGGCCTAGG GCCCAGAAGT 
TACTTCAGGT CTTCCTTCAA CTGCTTTGAC TTTGGGGTGA TTGTGGGGAG TATCTTTGAA 
GTAGTCTGGG CTGCCATCAA GCCAGGAACC TCCTTTGGAA TCAGTGTGCT GCGGGCTCTG 
CGACTGCTGA GGATATTCAA AGTTACCAAG TATTGGAACT CTCTGAGGAA CCTGGTGGTT 
TCCCTCCTCA ATTCCATGAA GTCCATCATC AGCCTTCTCT TCCTGCTTTT CCTCTTCATC 
GTGGTCTTCG CTCTGTTGGG GATGCAGCTG TTTGGGGGAC AGTTCAACTT TCAAGATGAG 
ACTCCAACCA CCAATTTTGA TACCTTCCCA GCTGCCATCC TCACTGTCTT TCAGATCCTG 
ACAGGAGAGG ATTGGAATGC CGTAATGTAT CATGGGATTG AGTCGCAAGG TGGAGTCAGC 
AAAGGCATGT TTTCTTCCTT TTACTTCATC GTCCTGACAC TGTTTGGAAA CTACACCCTG 
CTGAATGTTT TTCTGGCCAT TGCTGTGGAC AACCTTGCCA ATGCCCAGGA GTTGACCAAG 
GATGAAGAGG AGATGGAAGA AGCAGCCAAT CAGAAACTTG CTCTTCAGAA GGCCAAAGAA 
GTAGCTGAAG TCAGCCCCAT GTCTGCTGCC AATATCTCCA TCGCTGCGCA GCAGAACTCG 
GCCAAGGCGC GCTCAGTATG GGAGCAGCGG GCCAGTCAGC TAAGGCTCCA GAATCTGCGT 
GCCAGCTGTG AGGCATTGTA CAGTGAGATG GACCCTGAGG AGCGCCTGCG TTATGCCAGC 
ACGCGCCATG TGAGGCCAGA CATGAAGACA CACATGGACC GACCCCTAGT GGTGGAGCCT 
GGTCGAGATG GCTTGCGGGG ACCCGTTGGG AGCAAGTCAA AGCCTGAAGG CACGGAGGCC 
ACAGAAAGCG CGGACCTACC TCGCAGGCAC CACCGGCACC GTGATAGGGA CAAGACCTCA 
GCCACAGCAC CTGCTGGAGG CGAACAGGAC AGGACAGAAA GCACCGAGAC CGGGGCCCGG 
GAGGAACGTG CGCGCCCTCG TCGAAGTCAC AGCAAGGAGA CTCCAGGGGC TGACACGCAA 
GTGCGCTGTG AGCGCAGTAG ACGTCACCAC CGGCGCGGCT CCCCGGAGGA GGCCACTGAA 
CGGGAGCCTC GGCGCCACCG TGCCCACCGG CATGCACAGG ACTCAAGCAA GGAGGGCACG 
GCGCCGGTGC TTGTACCCAA GGGTGAGCGA CGAGCAAGAC ACCGAGGCCC ACGCACGGGT 
CCACGTGAGG CAGAGAACAA CGAGGAGCCC ACACGCAGGC ACCGTGCAAG GCATAAGGTG 
CCACCCACAC TGCAGCCCCC AGAGAGGGAG GCTGCAGAGA AGGAGAGCAA CGCGGTGGAA 
GGGGATAAGG AAACCCGAAA TCACCAGCCC AAGGAACCTC ACTGTGACCT GGAGGCCATT 
GCAGTTACAG GTGTGGGCCC TCTGCACATG CTGCCCAGCA CCTGTCTCCA GAAAGTGGAC 
GAGCAACCAG AGGATGCAGA CAACCAGCGT AATGTCACCC GGATGGGCAG TCAGCCCTCA 
GATCCCAGCA CCACTGTGCA TGTCCCAGTG ACACTGACAG GCCCTCCTGG GGAGACCCCT 
GTAGTTCCCA GTGGTAACAT GAACCTGGAA GGCCAAGCAG AGGGCAAGAA GGAGGCAGAG 
GCGGATGATG TGCTGAGAAG AGGCCCCAGG CCCATCGTTC CCTACAGCTC CATGTTTTGT 
CTCAGCCCCA CCAACCTGCT TCGTCGCTTC TGCCATTACA TTGTGACCAT GCGGTACTTT 
GAGATGGTAA TTCTTGTGGT CATTGCCTTG AGCAGCATTG CCCTGGCTGC AGAGGATCCT 
GTGCGGACAG ATTCATTCAG GAACAACGCT TTAAAGTACA TGGATTACAT CTTTACAGGA 
GTCTTCACCT TTGAAATGGT CATAAAGATG ATAGACTTGG GCTTGCTGCT GCACCCTGGT 
GCCTACTTCC GGGACCTGTG GAACATTCTG GACTTCATTG TTGTCAGTGG AGCCCTGGTG 
GCATTTGCGT TCTCAGGATC CAAAGGGAAA GACATCAATA CCATCAAGTC TCTGAGAGTC 
CTGCGTGTCC TGAGGCCCCT CAAGACCATC AAGCGGCTGC CTAAGCTCAA GGCTGTCTTT 
GACTGTGTGG TGAACTCCCT GAAGAACGTC TTGAACATCC TGATTGTCTA CATGCTCTTC 
ATGTTCATAT TTGCCGTCAT TGCCGTCCAG CTCTTCAAAG GGAAGTTCTT TTACTGTACT 
GATGAATCCA AGGAGCTGGA GAGGGACTGC CGGGGTCAGT ATTTGGATTA TGAGAAGGAA 
GAAGTAGAAG CCCAGCCAAG GCAGTGGAAG AAATATGACT TCCACTATGA CAATGTTCTC 
TGGGCCTTGT TGACGCTGTT CACAGTGTCC ACGGGAGAGG GGTGGCCCAT GGTGCTGAAA 
CACTCTGTGG ATGCCACCTA TGAGGAACAG GGGCCCAGTC CCGGGTTCCG GATGGAGCTC 
TCCATCTTCT ACGTGGTCTA CTTTGTGGTC TTCCCTTTTT TCTTTGTCAA CATCTTTGTG 
GCCTTGATCA TTATCACCTT CCAGGAACAG GGAGATAAGG TGATGTCTGA ATGCAGCTTA 
GAAAAGAATG AGAGGGCTTG CATTGATTTT GCCATCAGTG CCAAGCCCCT GACACGGTAC 
ATGCCTCAAA ACAAACAGTC GTTCCAGTAT AAGACATGGA CATTCGTGGT CTCTCCACCC 
TTTGAGTACT TCATCATGGC TATGATAGCC CTCAACACAG TGGTGCTGAT GATGAAGTTC 
TATGATGCAC CTTATGAGTA CGAGCTGATG CTGAAATGCC TGAACATTGT CTTCACATCC 
ATGTTCTCGA TGGAGTGCAT ACTGAAGATC ATCGCCTTTG GGGTATTGAA CTACTTCAGA 
GATGCCTGGA ACGTCTTTGA CTTTGTCACG GTTTTGGGAA GTATTACTGA TATTTTAGTA 
ACAGAGATAG CGGAAACGAA CAACTTCATC AACCTAAGCT TCCTTCGCCT CTTCCGGGCG 
GCACGGCTGA TCAAGCTGCT TCGCCAGGGC TACACCATCC GCATCCTATT GTGGACCTTC 
GTCCAGTCCT TTAAGGCGCT GCCCTACGTG TGCCTCCTCA TTGCCATGCT GTTCTTCATC 
TACGCCATCA TCGGCATGCA GGTTTTTGGA AACATTGCCC TTGATGATGA CACCAGTATC 
AACCGACACA ACAACTTCCG GACATTTCTG CAAGCCTTAA TGCTATTGTT CAGGAGTGCC 
ACTGGGGAGG CCTGGCATGA GATCATGCTG TCTTGTCTGG GCAACCGGGC CTGTGACCCA 
CATGCCAACG CCAGTGAGTG CGGGAGCGAC TTTGCCTATT TTTATTTTGT CTCCTTCATC 
TTCCTCTGTT CCTTTCTGAT GTTGAACCTC TTTGTTGCTG TAATCATGGA CAATTTTGAG 
TACCTCACTC GGGACTCTTC CATCCTAGGG CCTCACCACT TAGACGAATT CATTCGAGTC 
TGGGCTGAAT ACGACCCAGC TGCGTGTGGG CGCATCAGTT ACAATGACAT GTTTGAGATG 
CTGAAACACA TGTCCCCACC TCTGGGGTTG GGGAAGAAAT GCCCGGCTCG AGTTGCATAC 
AAGCGCCTGG TTCGCATGAA CATGCCCATA TCCAATGAGG ACATGACGGT GCACTTTACG 
TCCACACTGA TGGCCCTCAT CCGGACAGCA CTGGAGATCA AGCTTGCCCC AGCGGGGACG 
AAGCAGCACC AGTGTGATGC TGAGCTGAGA AAAGAGATCT CTTCTGTGTG GGCTAATCTG 
CCCCAGAAGA CTCTGGACTT ACTGGTACCA CCCCACAAAC CTGACGAGAT GACAGTGGGG 
AAGGTCTATG CTGCTCTCAT GATATTTGAC TTCTACAAAC AGAACAAAAC CACCAGAGAT 
CAGACTCACC AAGCTCCCGG AGGCCTGTCC CAGATGGGTC CCGTTTCCCT GTTCCACCCT 
CTGAAGGCCA CCCTGGAACA GACACAGCCC GCTGTGCTTC GAGGAGCTCG GGTTTTCCTT 
CGGCAAAAGA GTGCAACTTC CCTCAGCAAT GGGGGTGCCA TACAAACCCA GGAAAGTGGC 
ATCAAGGAGT CGCTGTCCTG GGGCACGCAG AGGACCCAAG ATGCACTTTA TGAGGCCAGA 
GCACCTCTAG AACGTGGCCA TTCTGCAGAG ATCCCTGTGG GGCAGTCAGG AACACTGGCT 
GTGGATGTCC AGATGCAGAA CATGACACTG AGAGGACCAG ATGGGGAGCC CCAGCCTGGG 
CTGGAAAGCC AAGGCAGAGC TGCCTCTATG CCACGCCTAG CGGCAGAAAC ACAGCCGGCC 
CCTAATGCCA GCCCCATGAA GCGCTCCATC TCCACACTGG CTCCACGCCC ACATGGGACT 
CAGCTTTGCA GCACAGTTCT GGACCGGCCT CCTCCTAGCC AGGCATCACA TCACCACCAC 
CACCGCTGCC ACCGGCGCAG AGACAAGAAG CAAAGGTCCC TGGAAAAGGG GCCCAGCCTG 
TCTGTTGACC CAGAAGGTGC ACCAAGCACT GCTGCAGGAC CTGGTCTGCC CCATGGAGAA 
GGATCCACCG CCTGCCGGCG GGACCGTAAA CAGGAGCGAG GCCGGTCCCA GGAGCGGAGG 
CAGCCCTCAT CTTCCTCTTC AGAGAAGCAG CGCTTCTATT CCTGTGACCG CTTTGGGAGC 
CGGGAGCCCC CGCAACTGAT GCCCTCACTC AGTAGCCACC CCACATCGCC AACAGCGGCG 
CTAGAGCCAG CACCCCACCC ACAGGGCAGT GGTTCCGTTA ATGGGAGCCC CTTGATGTCA 
ACATCCGGTG CTAGCACCCC GGGCCGAGGT GGGCGGAGGC AGCTCCCCCA GACTCCTCTG 
ACCCCACGCC CCAGCATCAC CTACAAGACC GCCAATTCCT CGCCTGTCCA CTTTGCTGAG 
GGTCAGAGCG GCCTCCCAGC CTTCTCCCCT GGCCGTCTCA GCCGCGGCCT TTCTGAACAC 
AATGCCCTGC TCCAGAAAGA GCCCCTGAGC CAGCCTCTAG CTCCTGGCTC CCGAATTGGC 
TCTGACCCTT ACCTAGGGCA GCGTCTGGAC AGTGAGGCCT CCGCCCACAC CCTGCCTGAG 
GATACACTCA CCTTTGAAGA GGCAGTGGCC ACCAACTCTG GCCGCTCCTC CAGGACTTCC 
TATGTGTCCT CCCTCACTTC CCAATCCCAC CCTCTCCGCC GTGTACCCAA TGGCTATCAC 
TGCACTTTGG GACTCAGCAC TGGCGTCCGG GCGCGGCACA GCTACCACCA CCCCGATCAG 
GACCACTGGT GCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cacna1b ( XM_006497635.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006497698.1 Mus musculus calcium channel, voltage-dependent, N type, alpha 1B subunit (Cacna1b), transcript variant X13, mRNA.

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CloneID	OMu29266
Clone ID Related Accession (Same CDS sequence)	XM_006497635.3
Accession Version	XM_006497635.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6831bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	voltage-dependent N-type calcium channel subunit alpha-1B isoform X13
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006497635.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTCCGCT TCGGGGACGA GCTAGGCGGC CGCTATGGGG GCACCGGCGG CGGGGAGCGG 
GCTCGGGGTG GCGGGGCCGG CGGGGCGGGT GGCCCGGGCC AGGGGGGTCT GCCGCCGGGC 
CAGCGGGTCC TGTACAAGCA GTCCATTGCG CAGCGCGCAC GGACTATGGC CCTGTACAAC 
CCCATCCCAG TCAAGCAGAA CTGCTTCACC GTCAACCGCT CGCTCTTCGT CTTCAGCGAG 
GACAACGTCG TCCGCAAATA CGCTAAGCGC ATCACCGAAT GGCCGCCCTT CGAATACATG 
ATCCTGGCCA CCATCATCGC CAACTGCATT GTTCTGGCCC TGGAGCAGCA CCTCCCTGAT 
GGGGACAAGA CTCCCATGTC TGAGCGACTA GATGACACGG AGCCTTACTT CATCGGGATC 
TTTTGCTTCG AGGCGGGCAT CAAGATCATA GCCCTGGGCT TTGTTTTCCA CAAGGGCTCC 
TACCTTCGGA ACGGCTGGAA TGTCATGGAC TTCGTGGTGG TCCTCACGGG GATTCTCGCC 
ACAGCTGGAA CTGACTTTGA CCTGCGCACA CTGAGGGCTG TGCGTGTGCT TAGGCCCCTG 
AAGCTGGTGT CTGGAATTCC AAGCTTGCAG GTGGTGCTTA AGTCCATCAT GAAGGCCATG 
GTCCCGCTGC TGCAGATTGG GCTGCTGCTC TTCTTTGCCA TCCTCATGTT TGCCATCATC 
GGCCTCGAAT TCTATATGGG CAAATTCCAT AAGGCCTGTT TCCCCAACAG CACAGATACA 
GAGCCTGTGG GTGACTTTCC CTGTGGCAAA GATCCCCCTG CTCGTCAGTG TGATGGTGAC 
ACCGAATGCC GGGAGTACTG GCCAGGACCC AACTTTGGCA TCACCAATTT TGACAACATC 
CTGTTTGCCA TCTTGACAGT GTTCCAGTGT ATCACCATGG AGGGCTGGAC TGACATCCTC 
TACAATACAA ATGATGCGGC TGGCAACACA TGGAACTGGT TGTACTTCAT CCCCCTCATC 
ATCATTGGCT CCTTCTTCAT GCTCAACCTG GTGCTGGGTG TGCTTTCCGG AGAGTTTGCC 
AAGGAGCGGG AGCGAGTCGA GAACCGCCGC GCCTTCCTGA AGCTCCGCAG GCAGCAGCAG 
ATTGAGCGAG AGCTGAATGG GTACTTGGAG TGGATCTTCA AGGCAGAGGA AGTCATGTTG 
GCAGAGGAGG ACAAGAATGC AGAAGAGAAA TCCCCTTTGG ATGCAGTGTT GAAGAGAGCT 
GCCACCAAGA AGAGCCGAAA TGACCTCATC CATGCAGAAG AGGGGGAGGA CCGGTTTGTA 
GACCTCTGTG CAGTTGGGTC TCCATTTGCT CGTGCCAGCC TCAAGAGTGG GAAGACGGAG 
AGCTCATCGT ACTTCCGGAG AAAGGAGAAG ATGTTCCGGT TCTTTATCCG GCGTATGGTG 
AAAGCACAGA GCTTCTACTG GGTGGTACTG TGTGTGGTGG CCCTGAACAC ACTGTGTGTG 
GCCATGGTGC ACTATAATCA GCCTCAGCGG CTTACCACTG CACTGTACTT TGCAGAGTTT 
GTTTTCCTGG GTCTCTTCCT CACAGAGATG TCCCTGAAGA TGTATGGCCT AGGGCCCAGA 
AGTTACTTCA GGTCTTCCTT CAACTGCTTT GACTTTGGGG TGATTGTGGG GAGTATCTTT 
GAAGTAGTCT GGGCTGCCAT CAAGCCAGGA ACCTCCTTTG GAATCAGTGT GCTGCGGGCT 
CTGCGACTGC TGAGGATATT CAAAGTTACC AAGTATTGGA ACTCTCTGAG GAACCTGGTG 
GTTTCCCTCC TCAATTCCAT GAAGTCCATC ATCAGCCTTC TCTTCCTGCT TTTCCTCTTC 
ATCGTGGTCT TCGCTCTGTT GGGGATGCAG CTGTTTGGGG GACAGTTCAA CTTTCAAGAT 
GAGACTCCAA CCACCAATTT TGATACCTTC CCAGCTGCCA TCCTCACTGT CTTTCAGATC 
CTGACAGGAG AGGATTGGAA TGCCGTAATG TATCATGGGA TTGAGTCGCA AGGTGGAGTC 
AGCAAAGGCA TGTTTTCTTC CTTTTACTTC ATCGTCCTGA CACTGTTTGG AAACTACACC 
CTGCTGAATG TTTTTCTGGC CATTGCTGTG GACAACCTTG CCAATGCCCA GGAGTTGACC 
AAGGATGAAG AGGAGATGGA AGAAGCAGCC AATCAGAAAC TTGCTCTTCA GAAGGCCAAA 
GAAGTAGCTG AAGTCAGCCC CATGTCTGCT GCCAATATCT CCATCGCTGC TTTTGTAAAG 
CAAACTCGAG GTACTGTATC TCGCAGCTCA TCTGTCTCCA GCGTAAACTC ACCCAGGCAG 
CAGAACTCGG CCAAGGCGCG CTCAGTATGG GAGCAGCGGG CCAGTCAGCT AAGGCTCCAG 
AATCTGCGTG CCAGCTGTGA GGCATTGTAC AGTGAGATGG ACCCTGAGGA GCGCCTGCGT 
TATGCCAGCA CGCGCCATGT GAGGCCAGAC ATGAAGACAC ACATGGACCG ACCCCTAGTG 
GTGGAGCCTG GTCGAGATGG CTTGCGGGGA CCCGTTGGGA GCAAGTCAAA GCCTGAAGGC 
ACGGAGGCCA CAGAAAGCGC GGACCTACCT CGCAGGCACC ACCGGCACCG TGATAGGGAC 
AAGACCTCAG CCACAGCACC TGCTGGAGGC GAACAGGACA GGACAGAAAG CACCGAGACC 
GGGGCCCGGG AGGAACGTGC GCGCCCTCGT CGAAGTCACA GCAAGGAGAC TCCAGGGGCT 
GACACGCAAG TGCGCTGTGA GCGCAGTAGA CGTCACCACC GGCGCGGCTC CCCGGAGGAG 
GCCACTGAAC GGGAGCCTCG GCGCCACCGT GCCCACCGGC ATGCACAGGA CTCAAGCAAG 
GAGGGCACGG CGCCGGTGCT TGTACCCAAG GGTGAGCGAC GAGCAAGACA CCGAGGCCCA 
CGCACGGGTC CACGTGAGGC AGAGAACAAC GAGGAGCCCA CACGCAGGCA CCGTGCAAGG 
CATAAGGTGC CACCCACACT GCAGCCCCCA GAGAGGGAGG CTGCAGAGAA GGAGAGCAAC 
GCGGTGGAAG GGGATAAGGA AACCCGAAAT CACCAGCCCA AGGAACCTCA CTGTGACCTG 
GAGGCCATTG CAGTTACAGG TGTGGGCCCT CTGCACATGC TGCCCAGCAC CTGTCTCCAG 
AAAGTGGACG AGCAACCAGA GGATGCAGAC AACCAGCGTA ATGTCACCCG GATGGGCAGT 
CAGCCCTCAG ATCCCAGCAC CACTGTGCAT GTCCCAGTGA CACTGACAGG CCCTCCTGGG 
GAGACCCCTG TAGTTCCCAG TGGTAACATG AACCTGGAAG GCCAAGCAGA GGGCAAGAAG 
GAGGCAGAGG CGGATGATGT GCTGAGAAGA GGCCCCAGGC CCATCGTTCC CTACAGCTCC 
ATGTTTTGTC TCAGCCCCAC CAACCTGCTT CGTCGCTTCT GCCATTACAT TGTGACCATG 
CGGTACTTTG AGATGGTAAT TCTTGTGGTC ATTGCCTTGA GCAGCATTGC CCTGGCTGCA 
GAGGATCCTG TGCGGACAGA TTCATTCAGG AACAACGCTT TAAAGTACAT GGATTACATC 
TTTACAGGAG TCTTCACCTT TGAAATGGTC ATAAAGATGA TAGACTTGGG CTTGCTGCTG 
CACCCTGGTG CCTACTTCCG GGACCTGTGG AACATTCTGG ACTTCATTGT TGTCAGTGGA 
GCCCTGGTGG CATTTGCGTT CTCGAGCTTC ATGGGAGGAT CCAAAGGGAA AGACATCAAT 
ACCATCAAGT CTCTGAGAGT CCTGCGTGTC CTGAGGCCCC TCAAGACCAT CAAGCGGCTG 
CCTAAGCTCA AGGCTGTCTT TGACTGTGTG GTGAACTCCC TGAAGAACGT CTTGAACATC 
CTGATTGTCT ACATGCTCTT CATGTTCATA TTTGCCGTCA TTGCCGTCCA GCTCTTCAAA 
GGGAAGTTCT TTTACTGTAC TGATGAATCC AAGGAGCTGG AGAGGGACTG CCGGGGTCAG 
TATTTGGATT ATGAGAAGGA AGAAGTAGAA GCCCAGCCAA GGCAGTGGAA GAAATATGAC 
TTCCACTATG ACAATGTTCT CTGGGCCTTG TTGACGCTGT TCACAGTGTC CACGGGAGAG 
GGGTGGCCCA TGGTGCTGAA ACACTCTGTG GATGCCACCT ATGAGGAACA GGGGCCCAGT 
CCCGGGTTCC GGATGGAGCT CTCCATCTTC TACGTGGTCT ACTTTGTGGT CTTCCCTTTT 
TTCTTTGTCA ACATCTTTGT GGCCTTGATC ATTATCACCT TCCAGGAACA GGGAGATAAG 
GTGATGTCTG AATGCAGCTT AGAAAAGAAT GAGAGGGCTT GCATTGATTT TGCCATCAGT 
GCCAAGCCCC TGACACGGTA CATGCCTCAA AACAAACAGT CGTTCCAGTA TAAGACATGG 
ACATTCGTGG TCTCTCCACC CTTTGAGTAC TTCATCATGG CTATGATAGC CCTCAACACA 
GTGGTGCTGA TGATGAAGTT CTATGATGCA CCTTATGAGT ACGAGCTGAT GCTGAAATGC 
CTGAACATTG TCTTCACATC CATGTTCTCG ATGGAGTGCA TACTGAAGAT CATCGCCTTT 
GGGGTATTGA ACTACTTCAG AGATGCCTGG AACGTCTTTG ACTTTGTCAC GGTTTTGGGA 
AGTATTACTG ATATTTTAGT AACAGAGATA GCGGAAACGA ACAACTTCAT CAACCTAAGC 
TTCCTTCGCC TCTTCCGGGC GGCACGGCTG ATCAAGCTGC TTCGCCAGGG CTACACCATC 
CGCATCCTAT TGTGGACCTT CGTCCAGTCC TTTAAGGCGC TGCCCTACGT GTGCCTCCTC 
ATTGCCATGC TGTTCTTCAT CTACGCCATC ATCGGCATGC AGGTTTTTGG AAACATTGCC 
CTTGATGATG ACACCAGTAT CAACCGACAC AACAACTTCC GGACATTTCT GCAAGCCTTA 
ATGCTATTGT TCAGGAGTGC CACTGGGGAG GCCTGGCATG AGATCATGCT GTCTTGTCTG 
GGCAACCGGG CCTGTGACCC ACATGCCAAC GCCAGTGAGT GCGGGAGCGA CTTTGCCTAT 
TTTTATTTTG TCTCCTTCAT CTTCCTCTGT TCCTTTCTGC GCCTGGTTCG CATGAACATG 
CCCATATCCA ATGAGGACAT GACGGTGCAC TTTACGTCCA CACTGATGGC CCTCATCCGG 
ACAGCACTGG AGATCAAGCT TGCCCCAGCG GGGACGAAGC AGCACCAGTG TGATGCTGAG 
CTGAGAAAAG AGATCTCTTC TGTGTGGGCT AATCTGCCCC AGAAGACTCT GGACTTACTG 
GTACCACCCC ACAAACCTGA CGAGATGACA GTGGGGAAGG TCTATGCTGC TCTCATGATA 
TTTGACTTCT ACAAACAGAA CAAAACCACC AGAGATCAGA CTCACCAAGC TCCCGGAGGC 
CTGTCCCAGA TGGGTCCCGT TTCCCTGTTC CACCCTCTGA AGGCCACCCT GGAACAGACA 
CAGCCCGCTG TGCTTCGAGG AGCTCGGGTT TTCCTTCGGC AAAAGAGTGC AACTTCCCTC 
AGCAATGGGG GTGCCATACA AACCCAGGAA AGTGGCATCA AGGAGTCGCT GTCCTGGGGC 
ACGCAGAGGA CCCAAGATGC ACTTTATGAG GCCAGAGCAC CTCTAGAACG TGGCCATTCT 
GCAGAGATCC CTGTGGGGCA GTCAGGAACA CTGGCTGTGG ATGTCCAGAT GCAGAACATG 
ACACTGAGAG GACCAGATGG GGAGCCCCAG CCTGGGCTGG AAAGCCAAGG CAGAGCTGCC 
TCTATGCCAC GCCTAGCGGC AGAAACACAG CCGGCCCCTA ATGCCAGCCC CATGAAGCGC 
TCCATCTCCA CACTGGCTCC ACGCCCACAT GGGACTCAGC TTTGCAGCAC AGTTCTGGAC 
CGGCCTCCTC CTAGCCAGGC ATCACATCAC CACCACCACC GCTGCCACCG GCGCAGAGAC 
AAGAAGCAAA GGTCCCTGGA AAAGGGGCCC AGCCTGTCTG TTGACCCAGA AGGTGCACCA 
AGCACTGCTG CAGGACCTGG TCTGCCCCAT GGAGAAGGAT CCACCGCCTG CCGGCGGGAC 
CGTAAACAGG AGCGAGGCCG GTCCCAGGAG CGGAGGCAGC CCTCATCTTC CTCTTCAGAG 
AAGCAGCGCT TCTATTCCTG TGACCGCTTT GGGAGCCGGG AGCCCCCGCA ACTGATGCCC 
TCACTCAGTA GCCACCCCAC ATCGCCAACA GCGGCGCTAG AGCCAGCACC CCACCCACAG 
GGCAGTGGTT CCGTTAATGG GAGCCCCTTG ATGTCAACAT CCGGTGCTAG CACCCCGGGC 
CGAGGTGGGC GGAGGCAGCT CCCCCAGACT CCTCTGACCC CACGCCCCAG CATCACCTAC 
AAGACCGCCA ATTCCTCGCC TGTCCACTTT GCTGAGGGTC AGAGCGGCCT CCCAGCCTTC 
TCCCCTGGCC GTCTCAGCCG CGGCCTTTCT GAACACAATG CCCTGCTCCA GAAAGAGCCC 
CTGAGCCAGC CTCTAGCTCC TGGCTCCCGA ATTGGCTCTG ACCCTTACCT AGGGCAGCGT 
CTGGACAGTG AGGCCTCCGC CCACACCCTG CCTGAGGATA CACTCACCTT TGAAGAGGCA 
GTGGCCACCA ACTCTGGCCG CTCCTCCAGG ACTTCCTATG TGTCCTCCCT CACTTCCCAA 
TCCCACCCTC TCCGCCGTGT ACCCAATGGC TATCACTGCA CTTTGGGACT CAGCACTGGC 
GTCCGGGCGC GGCACAGCTA CCACCACCCC GATCAGGACC ACTGGTGCTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006497698.1
CDS	146..6976
Translation	MVRFGDELGGRYGGTGGGERARGGGAGGAGGPGQGGLPPGQRVLYKQSIAQRARTMALYNPIPVKQNCFTVNRSLFVFSEDNVVRKYAKRITEWPPFEYMILATIIANCIVLALEQHLPDGDKTPMSERLDDTEPYFIGIFCFEAGIKIIALGFVFHKGSYLRNGWNVMDFVVVLTGILATAGTDFDLRTLRAVRVLRPLKLVSGIPSLQVVLKSIMKAMVPLLQIGLLLFFAILMFAIIGLEFYMGKFHKACFPNSTDTEPVGDFPCGKDPPARQCDGDTECREYWPGPNFGITNFDNILFAILTVFQCITMEGWTDILYNTNDAAGNTWNWLYFIPLIIIGSFFMLNLVLGVLSGEFAKERERVENRRAFLKLRRQQQIERELNGYLEWIFKAEEVMLAEEDKNAEEKSPLDAVLKRAATKKSRNDLIHAEEGEDRFVDLCAVGSPFARASLKSGKTESSSYFRRKEKMFRFFIRRMVKAQSFYWVVLCVVALNTLCVAMVHYNQPQRLTTALYFAEFVFLGLFLTEMSLKMYGLGPRSYFRSSFNCFDFGVIVGSIFEVVWAAIKPGTSFGISVLRALRLLRIFKVTKYWNSLRNLVVSLLNSMKSIISLLFLLFLFIVVFALLGMQLFGGQFNFQDETPTTNFDTFPAAILTVFQILTGEDWNAVMYHGIESQGGVSKGMFSSFYFIVLTLFGNYTLLNVFLAIAVDNLANAQELTKDEEEMEEAANQKLALQKAKEVAEVSPMSAANISIAAFVKQTRGTVSRSSSVSSVNSPRQQNSAKARSVWEQRASQLRLQNLRASCEALYSEMDPEERLRYASTRHVRPDMKTHMDRPLVVEPGRDGLRGPVGSKSKPEGTEATESADLPRRHHRHRDRDKTSATAPAGGEQDRTESTETGAREERARPRRSHSKETPGADTQVRCERSRRHHRRGSPEEATEREPRRHRAHRHAQDSSKEGTAPVLVPKGERRARHRGPRTGPREAENNEEPTRRHRARHKVPPTLQPPEREAAEKESNAVEGDKETRNHQPKEPHCDLEAIAVTGVGPLHMLPSTCLQKVDEQPEDADNQRNVTRMGSQPSDPSTTVHVPVTLTGPPGETPVVPSGNMNLEGQAEGKKEAEADDVLRRGPRPIVPYSSMFCLSPTNLLRRFCHYIVTMRYFEMVILVVIALSSIALAAEDPVRTDSFRNNALKYMDYIFTGVFTFEMVIKMIDLGLLLHPGAYFRDLWNILDFIVVSGALVAFAFSSFMGGSKGKDINTIKSLRVLRVLRPLKTIKRLPKLKAVFDCVVNSLKNVLNILIVYMLFMFIFAVIAVQLFKGKFFYCTDESKELERDCRGQYLDYEKEEVEAQPRQWKKYDFHYDNVLWALLTLFTVSTGEGWPMVLKHSVDATYEEQGPSPGFRMELSIFYVVYFVVFPFFFVNIFVALIIITFQEQGDKVMSECSLEKNERACIDFAISAKPLTRYMPQNKQSFQYKTWTFVVSPPFEYFIMAMIALNTVVLMMKFYDAPYEYELMLKCLNIVFTSMFSMECILKIIAFGVLNYFRDAWNVFDFVTVLGSITDILVTEIAETNNFINLSFLRLFRAARLIKLLRQGYTIRILLWTFVQSFKALPYVCLLIAMLFFIYAIIGMQVFGNIALDDDTSINRHNNFRTFLQALMLLFRSATGEAWHEIMLSCLGNRACDPHANASECGSDFAYFYFVSFIFLCSFLRLVRMNMPISNEDMTVHFTSTLMALIRTALEIKLAPAGTKQHQCDAELRKEISSVWANLPQKTLDLLVPPHKPDEMTVGKVYAALMIFDFYKQNKTTRDQTHQAPGGLSQMGPVSLFHPLKATLEQTQPAVLRGARVFLRQKSATSLSNGGAIQTQESGIKESLSWGTQRTQDALYEARAPLERGHSAEIPVGQSGTLAVDVQMQNMTLRGPDGEPQPGLESQGRAASMPRLAAETQPAPNASPMKRSISTLAPRPHGTQLCSTVLDRPPPSQASHHHHHRCHRRRDKKQRSLEKGPSLSVDPEGAPSTAAGPGLPHGEGSTACRRDRKQERGRSQERRQPSSSSSEKQRFYSCDRFGSREPPQLMPSLSSHPTSPTAALEPAPHPQGSGSVNGSPLMSTSGASTPGRGGRRQLPQTPLTPRPSITYKTANSSPVHFAEGQSGLPAFSPGRLSRGLSEHNALLQKEPLSQPLAPGSRIGSDPYLGQRLDSEASAHTLPEDTLTFEEAVATNSGRSSRTSYVSSLTSQSHPLRRVPNGYHCTLGLSTGVRARHSYHHPDQDHWC

Target ORF information:

RefSeq Version	XM_006497635.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus calcium channel, voltage-dependent, N type, alpha 1B subunit (Cacna1b), transcript variant X13, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006497635.3

ORF Insert Sequence:

ATGGTCCGCT TCGGGGACGA GCTAGGCGGC CGCTATGGGG GCACCGGCGG CGGGGAGCGG 
GCTCGGGGTG GCGGGGCCGG CGGGGCGGGT GGCCCGGGCC AGGGGGGTCT GCCGCCGGGC 
CAGCGGGTCC TGTACAAGCA GTCCATTGCG CAGCGCGCAC GGACTATGGC CCTGTACAAC 
CCCATCCCAG TCAAGCAGAA CTGCTTCACC GTCAACCGCT CGCTCTTCGT CTTCAGCGAG 
GACAACGTCG TCCGCAAATA CGCTAAGCGC ATCACCGAAT GGCCGCCCTT CGAATACATG 
ATCCTGGCCA CCATCATCGC CAACTGCATT GTTCTGGCCC TGGAGCAGCA CCTCCCTGAT 
GGGGACAAGA CTCCCATGTC TGAGCGACTA GATGACACGG AGCCTTACTT CATCGGGATC 
TTTTGCTTCG AGGCGGGCAT CAAGATCATA GCCCTGGGCT TTGTTTTCCA CAAGGGCTCC 
TACCTTCGGA ACGGCTGGAA TGTCATGGAC TTCGTGGTGG TCCTCACGGG GATTCTCGCC 
ACAGCTGGAA CTGACTTTGA CCTGCGCACA CTGAGGGCTG TGCGTGTGCT TAGGCCCCTG 
AAGCTGGTGT CTGGAATTCC AAGCTTGCAG GTGGTGCTTA AGTCCATCAT GAAGGCCATG 
GTCCCGCTGC TGCAGATTGG GCTGCTGCTC TTCTTTGCCA TCCTCATGTT TGCCATCATC 
GGCCTCGAAT TCTATATGGG CAAATTCCAT AAGGCCTGTT TCCCCAACAG CACAGATACA 
GAGCCTGTGG GTGACTTTCC CTGTGGCAAA GATCCCCCTG CTCGTCAGTG TGATGGTGAC 
ACCGAATGCC GGGAGTACTG GCCAGGACCC AACTTTGGCA TCACCAATTT TGACAACATC 
CTGTTTGCCA TCTTGACAGT GTTCCAGTGT ATCACCATGG AGGGCTGGAC TGACATCCTC 
TACAATACAA ATGATGCGGC TGGCAACACA TGGAACTGGT TGTACTTCAT CCCCCTCATC 
ATCATTGGCT CCTTCTTCAT GCTCAACCTG GTGCTGGGTG TGCTTTCCGG AGAGTTTGCC 
AAGGAGCGGG AGCGAGTCGA GAACCGCCGC GCCTTCCTGA AGCTCCGCAG GCAGCAGCAG 
ATTGAGCGAG AGCTGAATGG GTACTTGGAG TGGATCTTCA AGGCAGAGGA AGTCATGTTG 
GCAGAGGAGG ACAAGAATGC AGAAGAGAAA TCCCCTTTGG ATGCAGTGTT GAAGAGAGCT 
GCCACCAAGA AGAGCCGAAA TGACCTCATC CATGCAGAAG AGGGGGAGGA CCGGTTTGTA 
GACCTCTGTG CAGTTGGGTC TCCATTTGCT CGTGCCAGCC TCAAGAGTGG GAAGACGGAG 
AGCTCATCGT ACTTCCGGAG AAAGGAGAAG ATGTTCCGGT TCTTTATCCG GCGTATGGTG 
AAAGCACAGA GCTTCTACTG GGTGGTACTG TGTGTGGTGG CCCTGAACAC ACTGTGTGTG 
GCCATGGTGC ACTATAATCA GCCTCAGCGG CTTACCACTG CACTGTACTT TGCAGAGTTT 
GTTTTCCTGG GTCTCTTCCT CACAGAGATG TCCCTGAAGA TGTATGGCCT AGGGCCCAGA 
AGTTACTTCA GGTCTTCCTT CAACTGCTTT GACTTTGGGG TGATTGTGGG GAGTATCTTT 
GAAGTAGTCT GGGCTGCCAT CAAGCCAGGA ACCTCCTTTG GAATCAGTGT GCTGCGGGCT 
CTGCGACTGC TGAGGATATT CAAAGTTACC AAGTATTGGA ACTCTCTGAG GAACCTGGTG 
GTTTCCCTCC TCAATTCCAT GAAGTCCATC ATCAGCCTTC TCTTCCTGCT TTTCCTCTTC 
ATCGTGGTCT TCGCTCTGTT GGGGATGCAG CTGTTTGGGG GACAGTTCAA CTTTCAAGAT 
GAGACTCCAA CCACCAATTT TGATACCTTC CCAGCTGCCA TCCTCACTGT CTTTCAGATC 
CTGACAGGAG AGGATTGGAA TGCCGTAATG TATCATGGGA TTGAGTCGCA AGGTGGAGTC 
AGCAAAGGCA TGTTTTCTTC CTTTTACTTC ATCGTCCTGA CACTGTTTGG AAACTACACC 
CTGCTGAATG TTTTTCTGGC CATTGCTGTG GACAACCTTG CCAATGCCCA GGAGTTGACC 
AAGGATGAAG AGGAGATGGA AGAAGCAGCC AATCAGAAAC TTGCTCTTCA GAAGGCCAAA 
GAAGTAGCTG AAGTCAGCCC CATGTCTGCT GCCAATATCT CCATCGCTGC TTTTGTAAAG 
CAAACTCGAG GTACTGTATC TCGCAGCTCA TCTGTCTCCA GCGTAAACTC ACCCAGGCAG 
CAGAACTCGG CCAAGGCGCG CTCAGTATGG GAGCAGCGGG CCAGTCAGCT AAGGCTCCAG 
AATCTGCGTG CCAGCTGTGA GGCATTGTAC AGTGAGATGG ACCCTGAGGA GCGCCTGCGT 
TATGCCAGCA CGCGCCATGT GAGGCCAGAC ATGAAGACAC ACATGGACCG ACCCCTAGTG 
GTGGAGCCTG GTCGAGATGG CTTGCGGGGA CCCGTTGGGA GCAAGTCAAA GCCTGAAGGC 
ACGGAGGCCA CAGAAAGCGC GGACCTACCT CGCAGGCACC ACCGGCACCG TGATAGGGAC 
AAGACCTCAG CCACAGCACC TGCTGGAGGC GAACAGGACA GGACAGAAAG CACCGAGACC 
GGGGCCCGGG AGGAACGTGC GCGCCCTCGT CGAAGTCACA GCAAGGAGAC TCCAGGGGCT 
GACACGCAAG TGCGCTGTGA GCGCAGTAGA CGTCACCACC GGCGCGGCTC CCCGGAGGAG 
GCCACTGAAC GGGAGCCTCG GCGCCACCGT GCCCACCGGC ATGCACAGGA CTCAAGCAAG 
GAGGGCACGG CGCCGGTGCT TGTACCCAAG GGTGAGCGAC GAGCAAGACA CCGAGGCCCA 
CGCACGGGTC CACGTGAGGC AGAGAACAAC GAGGAGCCCA CACGCAGGCA CCGTGCAAGG 
CATAAGGTGC CACCCACACT GCAGCCCCCA GAGAGGGAGG CTGCAGAGAA GGAGAGCAAC 
GCGGTGGAAG GGGATAAGGA AACCCGAAAT CACCAGCCCA AGGAACCTCA CTGTGACCTG 
GAGGCCATTG CAGTTACAGG TGTGGGCCCT CTGCACATGC TGCCCAGCAC CTGTCTCCAG 
AAAGTGGACG AGCAACCAGA GGATGCAGAC AACCAGCGTA ATGTCACCCG GATGGGCAGT 
CAGCCCTCAG ATCCCAGCAC CACTGTGCAT GTCCCAGTGA CACTGACAGG CCCTCCTGGG 
GAGACCCCTG TAGTTCCCAG TGGTAACATG AACCTGGAAG GCCAAGCAGA GGGCAAGAAG 
GAGGCAGAGG CGGATGATGT GCTGAGAAGA GGCCCCAGGC CCATCGTTCC CTACAGCTCC 
ATGTTTTGTC TCAGCCCCAC CAACCTGCTT CGTCGCTTCT GCCATTACAT TGTGACCATG 
CGGTACTTTG AGATGGTAAT TCTTGTGGTC ATTGCCTTGA GCAGCATTGC CCTGGCTGCA 
GAGGATCCTG TGCGGACAGA TTCATTCAGG AACAACGCTT TAAAGTACAT GGATTACATC 
TTTACAGGAG TCTTCACCTT TGAAATGGTC ATAAAGATGA TAGACTTGGG CTTGCTGCTG 
CACCCTGGTG CCTACTTCCG GGACCTGTGG AACATTCTGG ACTTCATTGT TGTCAGTGGA 
GCCCTGGTGG CATTTGCGTT CTCGAGCTTC ATGGGAGGAT CCAAAGGGAA AGACATCAAT 
ACCATCAAGT CTCTGAGAGT CCTGCGTGTC CTGAGGCCCC TCAAGACCAT CAAGCGGCTG 
CCTAAGCTCA AGGCTGTCTT TGACTGTGTG GTGAACTCCC TGAAGAACGT CTTGAACATC 
CTGATTGTCT ACATGCTCTT CATGTTCATA TTTGCCGTCA TTGCCGTCCA GCTCTTCAAA 
GGGAAGTTCT TTTACTGTAC TGATGAATCC AAGGAGCTGG AGAGGGACTG CCGGGGTCAG 
TATTTGGATT ATGAGAAGGA AGAAGTAGAA GCCCAGCCAA GGCAGTGGAA GAAATATGAC 
TTCCACTATG ACAATGTTCT CTGGGCCTTG TTGACGCTGT TCACAGTGTC CACGGGAGAG 
GGGTGGCCCA TGGTGCTGAA ACACTCTGTG GATGCCACCT ATGAGGAACA GGGGCCCAGT 
CCCGGGTTCC GGATGGAGCT CTCCATCTTC TACGTGGTCT ACTTTGTGGT CTTCCCTTTT 
TTCTTTGTCA ACATCTTTGT GGCCTTGATC ATTATCACCT TCCAGGAACA GGGAGATAAG 
GTGATGTCTG AATGCAGCTT AGAAAAGAAT GAGAGGGCTT GCATTGATTT TGCCATCAGT 
GCCAAGCCCC TGACACGGTA CATGCCTCAA AACAAACAGT CGTTCCAGTA TAAGACATGG 
ACATTCGTGG TCTCTCCACC CTTTGAGTAC TTCATCATGG CTATGATAGC CCTCAACACA 
GTGGTGCTGA TGATGAAGTT CTATGATGCA CCTTATGAGT ACGAGCTGAT GCTGAAATGC 
CTGAACATTG TCTTCACATC CATGTTCTCG ATGGAGTGCA TACTGAAGAT CATCGCCTTT 
GGGGTATTGA ACTACTTCAG AGATGCCTGG AACGTCTTTG ACTTTGTCAC GGTTTTGGGA 
AGTATTACTG ATATTTTAGT AACAGAGATA GCGGAAACGA ACAACTTCAT CAACCTAAGC 
TTCCTTCGCC TCTTCCGGGC GGCACGGCTG ATCAAGCTGC TTCGCCAGGG CTACACCATC 
CGCATCCTAT TGTGGACCTT CGTCCAGTCC TTTAAGGCGC TGCCCTACGT GTGCCTCCTC 
ATTGCCATGC TGTTCTTCAT CTACGCCATC ATCGGCATGC AGGTTTTTGG AAACATTGCC 
CTTGATGATG ACACCAGTAT CAACCGACAC AACAACTTCC GGACATTTCT GCAAGCCTTA 
ATGCTATTGT TCAGGAGTGC CACTGGGGAG GCCTGGCATG AGATCATGCT GTCTTGTCTG 
GGCAACCGGG CCTGTGACCC ACATGCCAAC GCCAGTGAGT GCGGGAGCGA CTTTGCCTAT 
TTTTATTTTG TCTCCTTCAT CTTCCTCTGT TCCTTTCTGC GCCTGGTTCG CATGAACATG 
CCCATATCCA ATGAGGACAT GACGGTGCAC TTTACGTCCA CACTGATGGC CCTCATCCGG 
ACAGCACTGG AGATCAAGCT TGCCCCAGCG GGGACGAAGC AGCACCAGTG TGATGCTGAG 
CTGAGAAAAG AGATCTCTTC TGTGTGGGCT AATCTGCCCC AGAAGACTCT GGACTTACTG 
GTACCACCCC ACAAACCTGA CGAGATGACA GTGGGGAAGG TCTATGCTGC TCTCATGATA 
TTTGACTTCT ACAAACAGAA CAAAACCACC AGAGATCAGA CTCACCAAGC TCCCGGAGGC 
CTGTCCCAGA TGGGTCCCGT TTCCCTGTTC CACCCTCTGA AGGCCACCCT GGAACAGACA 
CAGCCCGCTG TGCTTCGAGG AGCTCGGGTT TTCCTTCGGC AAAAGAGTGC AACTTCCCTC 
AGCAATGGGG GTGCCATACA AACCCAGGAA AGTGGCATCA AGGAGTCGCT GTCCTGGGGC 
ACGCAGAGGA CCCAAGATGC ACTTTATGAG GCCAGAGCAC CTCTAGAACG TGGCCATTCT 
GCAGAGATCC CTGTGGGGCA GTCAGGAACA CTGGCTGTGG ATGTCCAGAT GCAGAACATG 
ACACTGAGAG GACCAGATGG GGAGCCCCAG CCTGGGCTGG AAAGCCAAGG CAGAGCTGCC 
TCTATGCCAC GCCTAGCGGC AGAAACACAG CCGGCCCCTA ATGCCAGCCC CATGAAGCGC 
TCCATCTCCA CACTGGCTCC ACGCCCACAT GGGACTCAGC TTTGCAGCAC AGTTCTGGAC 
CGGCCTCCTC CTAGCCAGGC ATCACATCAC CACCACCACC GCTGCCACCG GCGCAGAGAC 
AAGAAGCAAA GGTCCCTGGA AAAGGGGCCC AGCCTGTCTG TTGACCCAGA AGGTGCACCA 
AGCACTGCTG CAGGACCTGG TCTGCCCCAT GGAGAAGGAT CCACCGCCTG CCGGCGGGAC 
CGTAAACAGG AGCGAGGCCG GTCCCAGGAG CGGAGGCAGC CCTCATCTTC CTCTTCAGAG 
AAGCAGCGCT TCTATTCCTG TGACCGCTTT GGGAGCCGGG AGCCCCCGCA ACTGATGCCC 
TCACTCAGTA GCCACCCCAC ATCGCCAACA GCGGCGCTAG AGCCAGCACC CCACCCACAG 
GGCAGTGGTT CCGTTAATGG GAGCCCCTTG ATGTCAACAT CCGGTGCTAG CACCCCGGGC 
CGAGGTGGGC GGAGGCAGCT CCCCCAGACT CCTCTGACCC CACGCCCCAG CATCACCTAC 
AAGACCGCCA ATTCCTCGCC TGTCCACTTT GCTGAGGGTC AGAGCGGCCT CCCAGCCTTC 
TCCCCTGGCC GTCTCAGCCG CGGCCTTTCT GAACACAATG CCCTGCTCCA GAAAGAGCCC 
CTGAGCCAGC CTCTAGCTCC TGGCTCCCGA ATTGGCTCTG ACCCTTACCT AGGGCAGCGT 
CTGGACAGTG AGGCCTCCGC CCACACCCTG CCTGAGGATA CACTCACCTT TGAAGAGGCA 
GTGGCCACCA ACTCTGGCCG CTCCTCCAGG ACTTCCTATG TGTCCTCCCT CACTTCCCAA 
TCCCACCCTC TCCGCCGTGT ACCCAATGGC TATCACTGCA CTTTGGGACT CAGCACTGGC 
GTCCGGGCGC GGCACAGCTA CCACCACCCC GATCAGGACC ACTGGTGCTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cacna1b ( XM_006497637.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006497700.1 Mus musculus calcium channel, voltage-dependent, N type, alpha 1B subunit (Cacna1b), transcript variant X15, mRNA.

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CloneID	OMu29268
Clone ID Related Accession (Same CDS sequence)	XM_006497637.4 , XM_006497637.3
Accession Version	XM_006497637.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5427bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	voltage-dependent N-type calcium channel subunit alpha-1B isoform X15
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006497637.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTCCGCT TCGGGGACGA GCTAGGCGGC CGCTATGGGG GCACCGGCGG CGGGGAGCGG 
GCTCGGGGTG GCGGGGCCGG CGGGGCGGGT GGCCCGGGCC AGGGGGGTCT GCCGCCGGGC 
CAGCGGGTCC TGTACAAGCA GTCCATTGCG CAGCGCGCAC GGACTATGGC CCTGTACAAC 
CCCATCCCAG TCAAGCAGAA CTGCTTCACC GTCAACCGCT CGCTCTTCGT CTTCAGCGAG 
GACAACGTCG TCCGCAAATA CGCTAAGCGC ATCACCGAAT GGCCGCCCTT CGAATACATG 
ATCCTGGCCA CCATCATCGC CAACTGCATT GTTCTGGCCC TGGAGCAGCA CCTCCCTGAT 
GGGGACAAGA CTCCCATGTC TGAGCGACTA GATGACACGG AGCCTTACTT CATCGGGATC 
TTTTGCTTCG AGGCGGGCAT CAAGATCATA GCCCTGGGCT TTGTTTTCCA CAAGGGCTCC 
TACCTTCGGA ACGGCTGGAA TGTCATGGAC TTCGTGGTGG TCCTCACGGG GATTCTCGCC 
ACAGCTGGAA CTGACTTTGA CCTGCGCACA CTGAGGGCTG TGCGTGTGCT TAGGCCCCTG 
AAGCTGGTGT CTGGAATTCC AAGCTTGCAG GTGGTGCTTA AGTCCATCAT GAAGGCCATG 
GTCCCGCTGC TGCAGATTGG GCTGCTGCTC TTCTTTGCCA TCCTCATGTT TGCCATCATC 
GGCCTCGAAT TCTATATGGG CAAATTCCAT AAGGCCTGTT TCCCCAACAG CACAGATACA 
GAGCCTGTGG GTGACTTTCC CTGTGGCAAA GATCCCCCTG CTCGTCAGTG TGATGGTGAC 
ACCGAATGCC GGGAGTACTG GCCAGGACCC AACTTTGGCA TCACCAATTT TGACAACATC 
CTGTTTGCCA TCTTGACAGT GTTCCAGTGT ATCACCATGG AGGGCTGGAC TGACATCCTC 
TACAATACAA ATGATGCGGC TGGCAACACA TGGAACTGGT TGTACTTCAT CCCCCTCATC 
ATCATTGGCT CCTTCTTCAT GCTCAACCTG GTGCTGGGTG TGCTTTCCGG AGAGTTTGCC 
AAGGAGCGGG AGCGAGTCGA GAACCGCCGC GCCTTCCTGA AGCTCCGCAG GCAGCAGCAG 
ATTGAGCGAG AGCTGAATGG GTACTTGGAG TGGATCTTCA AGGCAGAGGA AGTCATGTTG 
GCAGAGGAGG ACAAGAATGC AGAAGAGAAA TCCCCTTTGG ATGCAGTGTT GAAGAGAGCT 
GCCACCAAGA AGAGCCGAAA TGACCTCATC CATGCAGAAG AGGGGGAGGA CCGGTTTGTA 
GACCTCTGTG CAGTTGGGTC TCCATTTGCT CGTGCCAGCC TCAAGAGTGG GAAGACGGAG 
AGCTCATCGT ACTTCCGGAG AAAGGAGAAG ATGTTCCGGT TCTTTATCCG GCGTATGGTG 
AAAGCACAGA GCTTCTACTG GGTGGTACTG TGTGTGGTGG CCCTGAACAC ACTGTGTGTG 
GCCATGGTGC ACTATAATCA GCCTCAGCGG CTTACCACTG CACTGTACTT TGCAGAGTTT 
GTTTTCCTGG GTCTCTTCCT CACAGAGATG TCCCTGAAGA TGTATGGCCT AGGGCCCAGA 
AGTTACTTCA GGTCTTCCTT CAACTGCTTT GACTTTGGGG TGATTGTGGG GAGTATCTTT 
GAAGTAGTCT GGGCTGCCAT CAAGCCAGGA ACCTCCTTTG GAATCAGTGT GCTGCGGGCT 
CTGCGACTGC TGAGGATATT CAAAGTTACC AAGTATTGGA ACTCTCTGAG GAACCTGGTG 
GTTTCCCTCC TCAATTCCAT GAAGTCCATC ATCAGCCTTC TCTTCCTGCT TTTCCTCTTC 
ATCGTGGTCT TCGCTCTGTT GGGGATGCAG CTGTTTGGGG GACAGTTCAA CTTTCAAGAT 
GAGACTCCAA CCACCAATTT TGATACCTTC CCAGCTGCCA TCCTCACTGT CTTTCAGATC 
CTGACAGGAG AGGATTGGAA TGCCGTAATG TATCATGGGA TTGAGTCGCA AGGTGGAGTC 
AGCAAAGGCA TGTTTTCTTC CTTTTACTTC ATCGTCCTGA CACTGTTTGG AAACTACACC 
CTGCTGAATG TTTTTCTGGC CATTGCTGTG GACAACCTTG CCAATGCCCA GGAGTTGACC 
AAGGATGAAG AGGAGATGGA AGAAGCAGCC AATCAGAAAC TTGCTCTTCA GAAGGCCAAA 
GAAGTAGCTG AAGTCAGCCC CATGTCTGCT GCCAATATCT CCATCGCTGC TTTTGTAAAG 
CAAACTCGAG GTACTGTATC TCGCAGCTCA TCTGTCTCCA GCGTAAACTC ACCCAGGCAG 
CAGAACTCGG CCAAGGCGCG CTCAGTATGG GAGCAGCGGG CCAGTCAGCT AAGGCTCCAG 
AATCTGCGTG CCAGCTGTGA GGCATTGTAC AGTGAGATGG ACCCTGAGGA GCGCCTGCGT 
TATGCCAGCA CGCGCCATGT GAGGCCAGAC ATGAAGACAC ACATGGACCG ACCCCTAGTG 
GTGGAGCCTG GTCGAGATGG CTTGCGGGGA CCCGTTGGGA GCAAGTCAAA GCCTGAAGGC 
ACGGAGGCCA CAGAAAGCGC GGACCTACCT CGCAGGCACC ACCGGCACCG TGATAGGGAC 
AAGACCTCAG CCACAGCACC TGCTGGAGGC GAACAGGACA GGACAGAAAG CACCGAGACC 
GGGGCCCGGG AGGAACGTGC GCGCCCTCGT CGAAGTCACA GCAAGGAGAC TCCAGGGGCT 
GACACGCAAG TGCGCTGTGA GCGCAGTAGA CGTCACCACC GGCGCGGCTC CCCGGAGGAG 
GCCACTGAAC GGGAGCCTCG GCGCCACCGT GCCCACCGGC ATGCACAGGA CTCAAGCAAG 
GAGGGCACGG CGCCGGTGCT TGTACCCAAG GGTGAGCGAC GAGCAAGACA CCGAGGCCCA 
CGCACGGGTC CACGTGAGGC AGAGAACAAC GAGGAGCCCA CACGCAGGCA CCGTGCAAGG 
CATAAGGTGC CACCCACACT GCAGCCCCCA GAGAGGGAGG CTGCAGAGAA GGAGAGCAAC 
GCGGTGGAAG GGGATAAGGA AACCCGAAAT CACCAGCCCA AGGAACCTCA CTGTGACCTG 
GAGGCCATTG CAGTTACAGG TGTGGGCCCT CTGCACATGC TGCCCAGCAC CTGTCTCCAG 
AAAGTGGACG AGCAACCAGA GGATGCAGAC AACCAGCGTA ATGTCACCCG GATGGGCAGT 
CAGCCCTCAG ATCCCAGCAC CACTGTGCAT GTCCCAGTGA CACTGACAGG CCCTCCTGGG 
GAGACCCCTG TAGTTCCCAG TGGTAACATG AACCTGGAAG GCCAAGCAGA GGGCAAGAAG 
GAGGCAGAGG CGGATGATGT GCTGAGAAGA GGCCCCAGGC CCATCGTTCC CTACAGCTCC 
ATGTTTTGTC TCAGCCCCAC CAACCTGCTT CGTCGCTTCT GCCATTACAT TGTGACCATG 
CGGTACTTTG AGATGGTAAT TCTTGTGGTC ATTGCCTTGA GCAGCATTGC CCTGGCTGCA 
GAGGATCCTG TGCGGACAGA TTCATTCAGG AACAACGCTT TAAAGTACAT GGATTACATC 
TTTACAGGAG TCTTCACCTT TGAAATGGTC ATAAAGATGA TAGACTTGGG CTTGCTGCTG 
CACCCTGGTG CCTACTTCCG GGACCTGTGG AACATTCTGG ACTTCATTGT TGTCAGTGGA 
GCCCTGGTGG CATTTGCGTT CTCGAGCTTC ATGGGAGGAT CCAAAGGGAA AGACATCAAT 
ACCATCAAGT CTCTGAGAGT CCTGCGTGTC CTGAGGCCCC TCAAGACCAT CAAGCGGCTG 
CCTAAGCTCA AGGCTGTCTT TGACTGTGTG GTGAACTCCC TGAAGAACGT CTTGAACATC 
CTGATTGTCT ACATGCTCTT CATGTTCATA TTTGCCGTCA TTGCCGTCCA GCTCTTCAAA 
GGGAAGTTCT TTTACTGTAC TGATGAATCC AAGGAGCTGG AGAGGGACTG CCGGGGTCAG 
TATTTGGATT ATGAGAAGGA AGAAGTAGAA GCCCAGCCAA GGCAGTGGAA GAAATATGAC 
TTCCACTATG ACAATGTTCT CTGGGCCTTG TTGACGCTGT TCACAGTGTC CACGGGAGAG 
GGGTGGCCCA TGGTGCTGAA ACACTCTGTG GATGCCACCT ATGAGGAACA GGGGCCCAGT 
CCCGGGTTCC GGATGGAGCT CTCCATCTTC TACGTGGTCT ACTTTGTGGT CTTCCCTTTT 
TTCTTTGTCA ACATCTTTGT GGCCTTGATC ATTATCACCT TCCAGGAACA GGGAGATAAG 
GTGATGTCTG AATGCAGCTT AGAAAAGAAT GAGAGGGCTT GCATTGATTT TGCCATCAGT 
GCCAAGCCCC TGACACGGTA CATGCCTCAA AACAAACAGT CGTTCCAGTA TAAGACATGG 
ACATTCGTGG TCTCTCCACC CTTTGAGTAC TTCATCATGG CTATGATAGC CCTCAACACA 
GTGGTGCTGA TGATGAAGTT CTATGATGCA CCTTATGAGT ACGAGCTGAT GCTGAAATGC 
CTGAACATTG TCTTCACATC CATGTTCTCG ATGGAGTGCA TACTGAAGAT CATCGCCTTT 
GGGGTATTGA ACTACTTCAG AGATGCCTGG AACGTCTTTG ACTTTGTCAC GGTTTTGGGA 
AGTATTACTG ATATTTTAGT AACAGAGATA GCGGAAACGA ACAACTTCAT CAACCTAAGC 
TTCCTTCGCC TCTTCCGGGC GGCACGGCTG ATCAAGCTGC TTCGCCAGGG CTACACCATC 
CGCATCCTAT TGTGGACCTT CGTCCAGTCC TTTAAGGCGC TGCCCTACGT GTGCCTCCTC 
ATTGCCATGC TGTTCTTCAT CTACGCCATC ATCGGCATGC AGGTTTTTGG AAACATTGCC 
CTTGATGATG ACACCAGTAT CAACCGACAC AACAACTTCC GGACATTTCT GCAAGCCTTA 
ATGCTATTGT TCAGGAGTGC CACTGGGGAG GCCTGGCATG AGATCATGCT GTCTTGTCTG 
GGCAACCGGG CCTGTGACCC ACATGCCAAC GCCAGTGAGT GCGGGAGCGA CTTTGCCTAT 
TTTTATTTTG TCTCCTTCAT CTTCCTCTGT TCCTTTCTGA TGTTGAACCT CTTTGTTGCT 
GTAATCATGG ACAATTTTGA GTACCTCACT CGGGACTCTT CCATCCTAGG GCCTCACCAC 
TTAGACGAAT TCATTCGAGT CTGGGCTGAA TACGACCCAG CTGCGTGCCA GGAGAATCTC 
AGCTCAGATG AGAGTGGGGT CCGGGAGGAG AGAATGCTGG CAAAACTTCC ACCCACCCAG 
CCTTCTGGAC CAGGCACCTG CCGGGACCAC TCTCAGGACG CCAAGCCAAG CTCCAGAGCC 
CAAACACTAA TTCTAGGGTG GGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006497700.1
CDS	153..5579
Translation	MVRFGDELGGRYGGTGGGERARGGGAGGAGGPGQGGLPPGQRVLYKQSIAQRARTMALYNPIPVKQNCFTVNRSLFVFSEDNVVRKYAKRITEWPPFEYMILATIIANCIVLALEQHLPDGDKTPMSERLDDTEPYFIGIFCFEAGIKIIALGFVFHKGSYLRNGWNVMDFVVVLTGILATAGTDFDLRTLRAVRVLRPLKLVSGIPSLQVVLKSIMKAMVPLLQIGLLLFFAILMFAIIGLEFYMGKFHKACFPNSTDTEPVGDFPCGKDPPARQCDGDTECREYWPGPNFGITNFDNILFAILTVFQCITMEGWTDILYNTNDAAGNTWNWLYFIPLIIIGSFFMLNLVLGVLSGEFAKERERVENRRAFLKLRRQQQIERELNGYLEWIFKAEEVMLAEEDKNAEEKSPLDAVLKRAATKKSRNDLIHAEEGEDRFVDLCAVGSPFARASLKSGKTESSSYFRRKEKMFRFFIRRMVKAQSFYWVVLCVVALNTLCVAMVHYNQPQRLTTALYFAEFVFLGLFLTEMSLKMYGLGPRSYFRSSFNCFDFGVIVGSIFEVVWAAIKPGTSFGISVLRALRLLRIFKVTKYWNSLRNLVVSLLNSMKSIISLLFLLFLFIVVFALLGMQLFGGQFNFQDETPTTNFDTFPAAILTVFQILTGEDWNAVMYHGIESQGGVSKGMFSSFYFIVLTLFGNYTLLNVFLAIAVDNLANAQELTKDEEEMEEAANQKLALQKAKEVAEVSPMSAANISIAAFVKQTRGTVSRSSSVSSVNSPRQQNSAKARSVWEQRASQLRLQNLRASCEALYSEMDPEERLRYASTRHVRPDMKTHMDRPLVVEPGRDGLRGPVGSKSKPEGTEATESADLPRRHHRHRDRDKTSATAPAGGEQDRTESTETGAREERARPRRSHSKETPGADTQVRCERSRRHHRRGSPEEATEREPRRHRAHRHAQDSSKEGTAPVLVPKGERRARHRGPRTGPREAENNEEPTRRHRARHKVPPTLQPPEREAAEKESNAVEGDKETRNHQPKEPHCDLEAIAVTGVGPLHMLPSTCLQKVDEQPEDADNQRNVTRMGSQPSDPSTTVHVPVTLTGPPGETPVVPSGNMNLEGQAEGKKEAEADDVLRRGPRPIVPYSSMFCLSPTNLLRRFCHYIVTMRYFEMVILVVIALSSIALAAEDPVRTDSFRNNALKYMDYIFTGVFTFEMVIKMIDLGLLLHPGAYFRDLWNILDFIVVSGALVAFAFSSFMGGSKGKDINTIKSLRVLRVLRPLKTIKRLPKLKAVFDCVVNSLKNVLNILIVYMLFMFIFAVIAVQLFKGKFFYCTDESKELERDCRGQYLDYEKEEVEAQPRQWKKYDFHYDNVLWALLTLFTVSTGEGWPMVLKHSVDATYEEQGPSPGFRMELSIFYVVYFVVFPFFFVNIFVALIIITFQEQGDKVMSECSLEKNERACIDFAISAKPLTRYMPQNKQSFQYKTWTFVVSPPFEYFIMAMIALNTVVLMMKFYDAPYEYELMLKCLNIVFTSMFSMECILKIIAFGVLNYFRDAWNVFDFVTVLGSITDILVTEIAETNNFINLSFLRLFRAARLIKLLRQGYTIRILLWTFVQSFKALPYVCLLIAMLFFIYAIIGMQVFGNIALDDDTSINRHNNFRTFLQALMLLFRSATGEAWHEIMLSCLGNRACDPHANASECGSDFAYFYFVSFIFLCSFLMLNLFVAVIMDNFEYLTRDSSILGPHHLDEFIRVWAEYDPAACQENLSSDESGVREERMLAKLPPTQPSGPGTCRDHSQDAKPSSRAQTLILGWE

Target ORF information:

RefSeq Version	XM_006497637.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus calcium channel, voltage-dependent, N type, alpha 1B subunit (Cacna1b), transcript variant X15, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006497637.3

ORF Insert Sequence:

ATGGTCCGCT TCGGGGACGA GCTAGGCGGC CGCTATGGGG GCACCGGCGG CGGGGAGCGG 
GCTCGGGGTG GCGGGGCCGG CGGGGCGGGT GGCCCGGGCC AGGGGGGTCT GCCGCCGGGC 
CAGCGGGTCC TGTACAAGCA GTCCATTGCG CAGCGCGCAC GGACTATGGC CCTGTACAAC 
CCCATCCCAG TCAAGCAGAA CTGCTTCACC GTCAACCGCT CGCTCTTCGT CTTCAGCGAG 
GACAACGTCG TCCGCAAATA CGCTAAGCGC ATCACCGAAT GGCCGCCCTT CGAATACATG 
ATCCTGGCCA CCATCATCGC CAACTGCATT GTTCTGGCCC TGGAGCAGCA CCTCCCTGAT 
GGGGACAAGA CTCCCATGTC TGAGCGACTA GATGACACGG AGCCTTACTT CATCGGGATC 
TTTTGCTTCG AGGCGGGCAT CAAGATCATA GCCCTGGGCT TTGTTTTCCA CAAGGGCTCC 
TACCTTCGGA ACGGCTGGAA TGTCATGGAC TTCGTGGTGG TCCTCACGGG GATTCTCGCC 
ACAGCTGGAA CTGACTTTGA CCTGCGCACA CTGAGGGCTG TGCGTGTGCT TAGGCCCCTG 
AAGCTGGTGT CTGGAATTCC AAGCTTGCAG GTGGTGCTTA AGTCCATCAT GAAGGCCATG 
GTCCCGCTGC TGCAGATTGG GCTGCTGCTC TTCTTTGCCA TCCTCATGTT TGCCATCATC 
GGCCTCGAAT TCTATATGGG CAAATTCCAT AAGGCCTGTT TCCCCAACAG CACAGATACA 
GAGCCTGTGG GTGACTTTCC CTGTGGCAAA GATCCCCCTG CTCGTCAGTG TGATGGTGAC 
ACCGAATGCC GGGAGTACTG GCCAGGACCC AACTTTGGCA TCACCAATTT TGACAACATC 
CTGTTTGCCA TCTTGACAGT GTTCCAGTGT ATCACCATGG AGGGCTGGAC TGACATCCTC 
TACAATACAA ATGATGCGGC TGGCAACACA TGGAACTGGT TGTACTTCAT CCCCCTCATC 
ATCATTGGCT CCTTCTTCAT GCTCAACCTG GTGCTGGGTG TGCTTTCCGG AGAGTTTGCC 
AAGGAGCGGG AGCGAGTCGA GAACCGCCGC GCCTTCCTGA AGCTCCGCAG GCAGCAGCAG 
ATTGAGCGAG AGCTGAATGG GTACTTGGAG TGGATCTTCA AGGCAGAGGA AGTCATGTTG 
GCAGAGGAGG ACAAGAATGC AGAAGAGAAA TCCCCTTTGG ATGCAGTGTT GAAGAGAGCT 
GCCACCAAGA AGAGCCGAAA TGACCTCATC CATGCAGAAG AGGGGGAGGA CCGGTTTGTA 
GACCTCTGTG CAGTTGGGTC TCCATTTGCT CGTGCCAGCC TCAAGAGTGG GAAGACGGAG 
AGCTCATCGT ACTTCCGGAG AAAGGAGAAG ATGTTCCGGT TCTTTATCCG GCGTATGGTG 
AAAGCACAGA GCTTCTACTG GGTGGTACTG TGTGTGGTGG CCCTGAACAC ACTGTGTGTG 
GCCATGGTGC ACTATAATCA GCCTCAGCGG CTTACCACTG CACTGTACTT TGCAGAGTTT 
GTTTTCCTGG GTCTCTTCCT CACAGAGATG TCCCTGAAGA TGTATGGCCT AGGGCCCAGA 
AGTTACTTCA GGTCTTCCTT CAACTGCTTT GACTTTGGGG TGATTGTGGG GAGTATCTTT 
GAAGTAGTCT GGGCTGCCAT CAAGCCAGGA ACCTCCTTTG GAATCAGTGT GCTGCGGGCT 
CTGCGACTGC TGAGGATATT CAAAGTTACC AAGTATTGGA ACTCTCTGAG GAACCTGGTG 
GTTTCCCTCC TCAATTCCAT GAAGTCCATC ATCAGCCTTC TCTTCCTGCT TTTCCTCTTC 
ATCGTGGTCT TCGCTCTGTT GGGGATGCAG CTGTTTGGGG GACAGTTCAA CTTTCAAGAT 
GAGACTCCAA CCACCAATTT TGATACCTTC CCAGCTGCCA TCCTCACTGT CTTTCAGATC 
CTGACAGGAG AGGATTGGAA TGCCGTAATG TATCATGGGA TTGAGTCGCA AGGTGGAGTC 
AGCAAAGGCA TGTTTTCTTC CTTTTACTTC ATCGTCCTGA CACTGTTTGG AAACTACACC 
CTGCTGAATG TTTTTCTGGC CATTGCTGTG GACAACCTTG CCAATGCCCA GGAGTTGACC 
AAGGATGAAG AGGAGATGGA AGAAGCAGCC AATCAGAAAC TTGCTCTTCA GAAGGCCAAA 
GAAGTAGCTG AAGTCAGCCC CATGTCTGCT GCCAATATCT CCATCGCTGC TTTTGTAAAG 
CAAACTCGAG GTACTGTATC TCGCAGCTCA TCTGTCTCCA GCGTAAACTC ACCCAGGCAG 
CAGAACTCGG CCAAGGCGCG CTCAGTATGG GAGCAGCGGG CCAGTCAGCT AAGGCTCCAG 
AATCTGCGTG CCAGCTGTGA GGCATTGTAC AGTGAGATGG ACCCTGAGGA GCGCCTGCGT 
TATGCCAGCA CGCGCCATGT GAGGCCAGAC ATGAAGACAC ACATGGACCG ACCCCTAGTG 
GTGGAGCCTG GTCGAGATGG CTTGCGGGGA CCCGTTGGGA GCAAGTCAAA GCCTGAAGGC 
ACGGAGGCCA CAGAAAGCGC GGACCTACCT CGCAGGCACC ACCGGCACCG TGATAGGGAC 
AAGACCTCAG CCACAGCACC TGCTGGAGGC GAACAGGACA GGACAGAAAG CACCGAGACC 
GGGGCCCGGG AGGAACGTGC GCGCCCTCGT CGAAGTCACA GCAAGGAGAC TCCAGGGGCT 
GACACGCAAG TGCGCTGTGA GCGCAGTAGA CGTCACCACC GGCGCGGCTC CCCGGAGGAG 
GCCACTGAAC GGGAGCCTCG GCGCCACCGT GCCCACCGGC ATGCACAGGA CTCAAGCAAG 
GAGGGCACGG CGCCGGTGCT TGTACCCAAG GGTGAGCGAC GAGCAAGACA CCGAGGCCCA 
CGCACGGGTC CACGTGAGGC AGAGAACAAC GAGGAGCCCA CACGCAGGCA CCGTGCAAGG 
CATAAGGTGC CACCCACACT GCAGCCCCCA GAGAGGGAGG CTGCAGAGAA GGAGAGCAAC 
GCGGTGGAAG GGGATAAGGA AACCCGAAAT CACCAGCCCA AGGAACCTCA CTGTGACCTG 
GAGGCCATTG CAGTTACAGG TGTGGGCCCT CTGCACATGC TGCCCAGCAC CTGTCTCCAG 
AAAGTGGACG AGCAACCAGA GGATGCAGAC AACCAGCGTA ATGTCACCCG GATGGGCAGT 
CAGCCCTCAG ATCCCAGCAC CACTGTGCAT GTCCCAGTGA CACTGACAGG CCCTCCTGGG 
GAGACCCCTG TAGTTCCCAG TGGTAACATG AACCTGGAAG GCCAAGCAGA GGGCAAGAAG 
GAGGCAGAGG CGGATGATGT GCTGAGAAGA GGCCCCAGGC CCATCGTTCC CTACAGCTCC 
ATGTTTTGTC TCAGCCCCAC CAACCTGCTT CGTCGCTTCT GCCATTACAT TGTGACCATG 
CGGTACTTTG AGATGGTAAT TCTTGTGGTC ATTGCCTTGA GCAGCATTGC CCTGGCTGCA 
GAGGATCCTG TGCGGACAGA TTCATTCAGG AACAACGCTT TAAAGTACAT GGATTACATC 
TTTACAGGAG TCTTCACCTT TGAAATGGTC ATAAAGATGA TAGACTTGGG CTTGCTGCTG 
CACCCTGGTG CCTACTTCCG GGACCTGTGG AACATTCTGG ACTTCATTGT TGTCAGTGGA 
GCCCTGGTGG CATTTGCGTT CTCGAGCTTC ATGGGAGGAT CCAAAGGGAA AGACATCAAT 
ACCATCAAGT CTCTGAGAGT CCTGCGTGTC CTGAGGCCCC TCAAGACCAT CAAGCGGCTG 
CCTAAGCTCA AGGCTGTCTT TGACTGTGTG GTGAACTCCC TGAAGAACGT CTTGAACATC 
CTGATTGTCT ACATGCTCTT CATGTTCATA TTTGCCGTCA TTGCCGTCCA GCTCTTCAAA 
GGGAAGTTCT TTTACTGTAC TGATGAATCC AAGGAGCTGG AGAGGGACTG CCGGGGTCAG 
TATTTGGATT ATGAGAAGGA AGAAGTAGAA GCCCAGCCAA GGCAGTGGAA GAAATATGAC 
TTCCACTATG ACAATGTTCT CTGGGCCTTG TTGACGCTGT TCACAGTGTC CACGGGAGAG 
GGGTGGCCCA TGGTGCTGAA ACACTCTGTG GATGCCACCT ATGAGGAACA GGGGCCCAGT 
CCCGGGTTCC GGATGGAGCT CTCCATCTTC TACGTGGTCT ACTTTGTGGT CTTCCCTTTT 
TTCTTTGTCA ACATCTTTGT GGCCTTGATC ATTATCACCT TCCAGGAACA GGGAGATAAG 
GTGATGTCTG AATGCAGCTT AGAAAAGAAT GAGAGGGCTT GCATTGATTT TGCCATCAGT 
GCCAAGCCCC TGACACGGTA CATGCCTCAA AACAAACAGT CGTTCCAGTA TAAGACATGG 
ACATTCGTGG TCTCTCCACC CTTTGAGTAC TTCATCATGG CTATGATAGC CCTCAACACA 
GTGGTGCTGA TGATGAAGTT CTATGATGCA CCTTATGAGT ACGAGCTGAT GCTGAAATGC 
CTGAACATTG TCTTCACATC CATGTTCTCG ATGGAGTGCA TACTGAAGAT CATCGCCTTT 
GGGGTATTGA ACTACTTCAG AGATGCCTGG AACGTCTTTG ACTTTGTCAC GGTTTTGGGA 
AGTATTACTG ATATTTTAGT AACAGAGATA GCGGAAACGA ACAACTTCAT CAACCTAAGC 
TTCCTTCGCC TCTTCCGGGC GGCACGGCTG ATCAAGCTGC TTCGCCAGGG CTACACCATC 
CGCATCCTAT TGTGGACCTT CGTCCAGTCC TTTAAGGCGC TGCCCTACGT GTGCCTCCTC 
ATTGCCATGC TGTTCTTCAT CTACGCCATC ATCGGCATGC AGGTTTTTGG AAACATTGCC 
CTTGATGATG ACACCAGTAT CAACCGACAC AACAACTTCC GGACATTTCT GCAAGCCTTA 
ATGCTATTGT TCAGGAGTGC CACTGGGGAG GCCTGGCATG AGATCATGCT GTCTTGTCTG 
GGCAACCGGG CCTGTGACCC ACATGCCAAC GCCAGTGAGT GCGGGAGCGA CTTTGCCTAT 
TTTTATTTTG TCTCCTTCAT CTTCCTCTGT TCCTTTCTGA TGTTGAACCT CTTTGTTGCT 
GTAATCATGG ACAATTTTGA GTACCTCACT CGGGACTCTT CCATCCTAGG GCCTCACCAC 
TTAGACGAAT TCATTCGAGT CTGGGCTGAA TACGACCCAG CTGCGTGCCA GGAGAATCTC 
AGCTCAGATG AGAGTGGGGT CCGGGAGGAG AGAATGCTGG CAAAACTTCC ACCCACCCAG 
CCTTCTGGAC CAGGCACCTG CCGGGACCAC TCTCAGGACG CCAAGCCAAG CTCCAGAGCC 
CAAACACTAA TTCTAGGGTG GGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cacna1b ( XM_006497638.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006497701.1 Mus musculus calcium channel, voltage-dependent, N type, alpha 1B subunit (Cacna1b), transcript variant X16, mRNA.

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CloneID	OMu29269
Clone ID Related Accession (Same CDS sequence)	XM_006497638.3
Accession Version	XM_006497638.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5391bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	voltage-dependent N-type calcium channel subunit alpha-1B isoform X16
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006497638.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTCCGCT TCGGGGACGA GCTAGGCGGC CGCTATGGGG GCACCGGCGG CGGGGAGCGG 
GCTCGGGGTG GCGGGGCCGG CGGGGCGGGT GGCCCGGGCC AGGGGGGTCT GCCGCCGGGC 
CAGCGGGTCC TGTACAAGCA GTCCATTGCG CAGCGCGCAC GGACTATGGC CCTGTACAAC 
CCCATCCCAG TCAAGCAGAA CTGCTTCACC GTCAACCGCT CGCTCTTCGT CTTCAGCGAG 
GACAACGTCG TCCGCAAATA CGCTAAGCGC ATCACCGAAT GGCCGCCCTT CGAATACATG 
ATCCTGGCCA CCATCATCGC CAACTGCATT GTTCTGGCCC TGGAGCAGCA CCTCCCTGAT 
GGGGACAAGA CTCCCATGTC TGAGCGACTA GATGACACGG AGCCTTACTT CATCGGGATC 
TTTTGCTTCG AGGCGGGCAT CAAGATCATA GCCCTGGGCT TTGTTTTCCA CAAGGGCTCC 
TACCTTCGGA ACGGCTGGAA TGTCATGGAC TTCGTGGTGG TCCTCACGGG GATTCTCGCC 
ACAGCTGGAA CTGACTTTGA CCTGCGCACA CTGAGGGCTG TGCGTGTGCT TAGGCCCCTG 
AAGCTGGTGT CTGGAATTCC AAGCTTGCAG GTGGTGCTTA AGTCCATCAT GAAGGCCATG 
GTCCCGCTGC TGCAGATTGG GCTGCTGCTC TTCTTTGCCA TCCTCATGTT TGCCATCATC 
GGCCTCGAAT TCTATATGGG CAAATTCCAT AAGGCCTGTT TCCCCAACAG CACAGATACA 
GAGCCTGTGG GTGACTTTCC CTGTGGCAAA GATCCCCCTG CTCGTCAGTG TGATGGTGAC 
ACCGAATGCC GGGAGTACTG GCCAGGACCC AACTTTGGCA TCACCAATTT TGACAACATC 
CTGTTTGCCA TCTTGACAGT GTTCCAGTGT ATCACCATGG AGGGCTGGAC TGACATCCTC 
TACAATACAA ATGATGCGGC TGGCAACACA TGGAACTGGT TGTACTTCAT CCCCCTCATC 
ATCATTGGCT CCTTCTTCAT GCTCAACCTG GTGCTGGGTG TGCTTTCCGG AGAGTTTGCC 
AAGGAGCGGG AGCGAGTCGA GAACCGCCGC GCCTTCCTGA AGCTCCGCAG GCAGCAGCAG 
ATTGAGCGAG AGCTGAATGG GTACTTGGAG TGGATCTTCA AGGCAGAGGA AGTCATGTTG 
GCAGAGGAGG ACAAGAATGC AGAAGAGAAA TCCCCTTTGG ATGCAGTGTT GAAGAGAGCT 
GCCACCAAGA AGAGCCGAAA TGACCTCATC CATGCAGAAG AGGGGGAGGA CCGGTTTGTA 
GACCTCTGTG CAGTTGGGTC TCCATTTGCT CGTGCCAGCC TCAAGAGTGG GAAGACGGAG 
AGCTCATCGT ACTTCCGGAG AAAGGAGAAG ATGTTCCGGT TCTTTATCCG GCGTATGGTG 
AAAGCACAGA GCTTCTACTG GGTGGTACTG TGTGTGGTGG CCCTGAACAC ACTGTGTGTG 
GCCATGGTGC ACTATAATCA GCCTCAGCGG CTTACCACTG CACTGTACTT TGCAGAGTTT 
GTTTTCCTGG GTCTCTTCCT CACAGAGATG TCCCTGAAGA TGTATGGCCT AGGGCCCAGA 
AGTTACTTCA GGTCTTCCTT CAACTGCTTT GACTTTGGGG TGATTGTGGG GAGTATCTTT 
GAAGTAGTCT GGGCTGCCAT CAAGCCAGGA ACCTCCTTTG GAATCAGTGT GCTGCGGGCT 
CTGCGACTGC TGAGGATATT CAAAGTTACC AAGTATTGGA ACTCTCTGAG GAACCTGGTG 
GTTTCCCTCC TCAATTCCAT GAAGTCCATC ATCAGCCTTC TCTTCCTGCT TTTCCTCTTC 
ATCGTGGTCT TCGCTCTGTT GGGGATGCAG CTGTTTGGGG GACAGTTCAA CTTTCAAGAT 
GAGACTCCAA CCACCAATTT TGATACCTTC CCAGCTGCCA TCCTCACTGT CTTTCAGATC 
CTGACAGGAG AGGATTGGAA TGCCGTAATG TATCATGGGA TTGAGTCGCA AGGTGGAGTC 
AGCAAAGGCA TGTTTTCTTC CTTTTACTTC ATCGTCCTGA CACTGTTTGG AAACTACACC 
CTGCTGAATG TTTTTCTGGC CATTGCTGTG GACAACCTTG CCAATGCCCA GGAGTTGACC 
AAGGATGAAG AGGAGATGGA AGAAGCAGCC AATCAGAAAC TTGCTCTTCA GAAGGCCAAA 
GAAGTAGCTG AAGTCAGCCC CATGTCTGCT GCCAATATCT CCATCGCTGC TTTTGTAAAG 
CAAACTCGAG GTACTGTATC TCGCAGCTCA TCTGTCTCCA GCGTAAACTC ACCCAGGCAG 
CAGAACTCGG CCAAGGCGCG CTCAGTATGG GAGCAGCGGG CCAGTCAGCT AAGGCTCCAG 
AATCTGCGTG CCAGCTGTGA GGCATTGTAC AGTGAGATGG ACCCTGAGGA GCGCCTGCGT 
TATGCCAGCA CGCGCCATGT GAGGCCAGAC ATGAAGACAC ACATGGACCG ACCCCTAGTG 
GTGGAGCCTG GTCGAGATGG CTTGCGGGGA CCCGTTGGGA GCAAGTCAAA GCCTGAAGGC 
ACGGAGGCCA CAGAAAGCGC GGACCTACCT CGCAGGCACC ACCGGCACCG TGATAGGGAC 
AAGACCTCAG CCACAGCACC TGCTGGAGGC GAACAGGACA GGACAGAAAG CACCGAGACC 
GGGGCCCGGG AGGAACGTGC GCGCCCTCGT CGAAGTCACA GCAAGGAGAC TCCAGGGGCT 
GACACGCAAG TGCGCTGTGA GCGCAGTAGA CGTCACCACC GGCGCGGCTC CCCGGAGGAG 
GCCACTGAAC GGGAGCCTCG GCGCCACCGT GCCCACCGGC ATGCACAGGA CTCAAGCAAG 
GAGGGCACGG CGCCGGTGCT TGTACCCAAG GGTGAGCGAC GAGCAAGACA CCGAGGCCCA 
CGCACGGGTC CACGTGAGGC AGAGAACAAC GAGGAGCCCA CACGCAGGCA CCGTGCAAGG 
CATAAGGTGC CACCCACACT GCAGCCCCCA GAGAGGGAGG CTGCAGAGAA GGAGAGCAAC 
GCGGTGGAAG GGGATAAGGA AACCCGAAAT CACCAGCCCA AGGAACCTCA CTGTGACCTG 
GAGGCCATTG CAGTTACAGG TGTGGGCCCT CTGCACATGC TGCCCAGCAC CTGTCTCCAG 
AAAGTGGACG AGCAACCAGA GGATGCAGAC AACCAGCGTA ATGTCACCCG GATGGGCAGT 
CAGCCCTCAG ATCCCAGCAC CACTGTGCAT GTCCCAGTGA CACTGACAGG CCCTCCTGGG 
GAGACCCCTG TAGTTCCCAG TGGTAACATG AACCTGGAAG GCCAAGCAGA GGGCAAGAAG 
GAGGCAGAGG CGGATGATGT GCTGAGAAGA GGCCCCAGGC CCATCGTTCC CTACAGCTCC 
ATGTTTTGTC TCAGCCCCAC CAACCTGCTT CGTCGCTTCT GCCATTACAT TGTGACCATG 
CGGTACTTTG AGATGGTAAT TCTTGTGGTC ATTGCCTTGA GCAGCATTGC CCTGGCTGCA 
GAGGATCCTG TGCGGACAGA TTCATTCAGG AACAACGCTT TAAAGTACAT GGATTACATC 
TTTACAGGAG TCTTCACCTT TGAAATGGTC ATAAAGATGA TAGACTTGGG CTTGCTGCTG 
CACCCTGGTG CCTACTTCCG GGACCTGTGG AACATTCTGG ACTTCATTGT TGTCAGTGGA 
GCCCTGGTGG CATTTGCGTT CTCGAGCTTC ATGGGAGGAT CCAAAGGGAA AGACATCAAT 
ACCATCAAGT CTCTGAGAGT CCTGCGTGTC CTGAGGCCCC TCAAGACCAT CAAGCGGCTG 
CCTAAGCTCA AGGCTGTCTT TGACTGTGTG GTGAACTCCC TGAAGAACGT CTTGAACATC 
CTGATTGTCT ACATGCTCTT CATGTTCATA TTTGCCGTCA TTGCCGTCCA GCTCTTCAAA 
GGGAAGTTCT TTTACTGTAC TGATGAATCC AAGGAGCTGG AGAGGGACTG CCGGGGTCAG 
TATTTGGATT ATGAGAAGGA AGAAGTAGAA GCCCAGCCAA GGCAGTGGAA GAAATATGAC 
TTCCACTATG ACAATGTTCT CTGGGCCTTG TTGACGCTGT TCACAGTGTC CACGGGAGAG 
GGGTGGCCCA TGGTGCTGAA ACACTCTGTG GATGCCACCT ATGAGGAACA GGGGCCCAGT 
CCCGGGTTCC GGATGGAGCT CTCCATCTTC TACGTGGTCT ACTTTGTGGT CTTCCCTTTT 
TTCTTTGTCA ACATCTTTGT GGCCTTGATC ATTATCACCT TCCAGGAACA GGGAGATAAG 
GTGATGTCTG AATGCAGCTT AGAAAAGAAT GAGAGGGCTT GCATTGATTT TGCCATCAGT 
GCCAAGCCCC TGACACGGTA CATGCCTCAA AACAAACAGT CGTTCCAGTA TAAGACATGG 
ACATTCGTGG TCTCTCCACC CTTTGAGTAC TTCATCATGG CTATGATAGC CCTCAACACA 
GTGGTGCTGA TGATGAAGTT CTATGATGCA CCTTATGAGT ACGAGCTGAT GCTGAAATGC 
CTGAACATTG TCTTCACATC CATGTTCTCG ATGGAGTGCA TACTGAAGAT CATCGCCTTT 
GGGGTATTGA ACTACTTCAG AGATGCCTGG AACGTCTTTG ACTTTGTCAC GGTTTTGGGA 
AGTATTACTG ATATTTTAGT AACAGAGATA GCGGAAACGA ACAACTTCAT CAACCTAAGC 
TTCCTTCGCC TCTTCCGGGC GGCACGGCTG ATCAAGCTGC TTCGCCAGGG CTACACCATC 
CGCATCCTAT TGTGGACCTT CGTCCAGTCC TTTAAGGCGC TGCCCTACGT GTGCCTCCTC 
ATTGCCATGC TGTTCTTCAT CTACGCCATC ATCGGCATGC AGGTTTTTGG AAACATTGCC 
CTTGATGATG ACACCAGTAT CAACCGACAC AACAACTTCC GGACATTTCT GCAAGCCTTA 
ATGCTATTGT TCAGGAGTGC CACTGGGGAG GCCTGGCATG AGATCATGCT GTCTTGTCTG 
GGCAACCGGG CCTGTGACCC ACATGCCAAC GCCAGTGAGT GCGGGAGCGA CTTTGCCTAT 
TTTTATTTTG TCTCCTTCAT CTTCCTCTGT TCCTTTCTGA TGTTGAACCT CTTTGTTGCT 
GTAATCATGG ACAATTTTGA GTACCTCACT CGGGACTCTT CCATCCTAGG GCCTCACCAC 
TTAGACGAAT TCATTCGAGT CTGGGCTGAA TACGACCCAG CTGCGTGTTG CCGGATTCAT 
TATAAGGATA TGTACAGTTT GTTGCGTTGT ATTGCGCCAC CCGTTGGCTT AGGAAAGAAC 
TGCCCTCGTA GGTTGGCCTA CAAGGTTTGC AACTTCAGAG CCTCCATCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006497701.1
CDS	153..5543
Translation	MVRFGDELGGRYGGTGGGERARGGGAGGAGGPGQGGLPPGQRVLYKQSIAQRARTMALYNPIPVKQNCFTVNRSLFVFSEDNVVRKYAKRITEWPPFEYMILATIIANCIVLALEQHLPDGDKTPMSERLDDTEPYFIGIFCFEAGIKIIALGFVFHKGSYLRNGWNVMDFVVVLTGILATAGTDFDLRTLRAVRVLRPLKLVSGIPSLQVVLKSIMKAMVPLLQIGLLLFFAILMFAIIGLEFYMGKFHKACFPNSTDTEPVGDFPCGKDPPARQCDGDTECREYWPGPNFGITNFDNILFAILTVFQCITMEGWTDILYNTNDAAGNTWNWLYFIPLIIIGSFFMLNLVLGVLSGEFAKERERVENRRAFLKLRRQQQIERELNGYLEWIFKAEEVMLAEEDKNAEEKSPLDAVLKRAATKKSRNDLIHAEEGEDRFVDLCAVGSPFARASLKSGKTESSSYFRRKEKMFRFFIRRMVKAQSFYWVVLCVVALNTLCVAMVHYNQPQRLTTALYFAEFVFLGLFLTEMSLKMYGLGPRSYFRSSFNCFDFGVIVGSIFEVVWAAIKPGTSFGISVLRALRLLRIFKVTKYWNSLRNLVVSLLNSMKSIISLLFLLFLFIVVFALLGMQLFGGQFNFQDETPTTNFDTFPAAILTVFQILTGEDWNAVMYHGIESQGGVSKGMFSSFYFIVLTLFGNYTLLNVFLAIAVDNLANAQELTKDEEEMEEAANQKLALQKAKEVAEVSPMSAANISIAAFVKQTRGTVSRSSSVSSVNSPRQQNSAKARSVWEQRASQLRLQNLRASCEALYSEMDPEERLRYASTRHVRPDMKTHMDRPLVVEPGRDGLRGPVGSKSKPEGTEATESADLPRRHHRHRDRDKTSATAPAGGEQDRTESTETGAREERARPRRSHSKETPGADTQVRCERSRRHHRRGSPEEATEREPRRHRAHRHAQDSSKEGTAPVLVPKGERRARHRGPRTGPREAENNEEPTRRHRARHKVPPTLQPPEREAAEKESNAVEGDKETRNHQPKEPHCDLEAIAVTGVGPLHMLPSTCLQKVDEQPEDADNQRNVTRMGSQPSDPSTTVHVPVTLTGPPGETPVVPSGNMNLEGQAEGKKEAEADDVLRRGPRPIVPYSSMFCLSPTNLLRRFCHYIVTMRYFEMVILVVIALSSIALAAEDPVRTDSFRNNALKYMDYIFTGVFTFEMVIKMIDLGLLLHPGAYFRDLWNILDFIVVSGALVAFAFSSFMGGSKGKDINTIKSLRVLRVLRPLKTIKRLPKLKAVFDCVVNSLKNVLNILIVYMLFMFIFAVIAVQLFKGKFFYCTDESKELERDCRGQYLDYEKEEVEAQPRQWKKYDFHYDNVLWALLTLFTVSTGEGWPMVLKHSVDATYEEQGPSPGFRMELSIFYVVYFVVFPFFFVNIFVALIIITFQEQGDKVMSECSLEKNERACIDFAISAKPLTRYMPQNKQSFQYKTWTFVVSPPFEYFIMAMIALNTVVLMMKFYDAPYEYELMLKCLNIVFTSMFSMECILKIIAFGVLNYFRDAWNVFDFVTVLGSITDILVTEIAETNNFINLSFLRLFRAARLIKLLRQGYTIRILLWTFVQSFKALPYVCLLIAMLFFIYAIIGMQVFGNIALDDDTSINRHNNFRTFLQALMLLFRSATGEAWHEIMLSCLGNRACDPHANASECGSDFAYFYFVSFIFLCSFLMLNLFVAVIMDNFEYLTRDSSILGPHHLDEFIRVWAEYDPAACCRIHYKDMYSLLRCIAPPVGLGKNCPRRLAYKVCNFRASI

Target ORF information:

RefSeq Version	XM_006497638.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus calcium channel, voltage-dependent, N type, alpha 1B subunit (Cacna1b), transcript variant X16, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006497638.3

ORF Insert Sequence:

ATGGTCCGCT TCGGGGACGA GCTAGGCGGC CGCTATGGGG GCACCGGCGG CGGGGAGCGG 
GCTCGGGGTG GCGGGGCCGG CGGGGCGGGT GGCCCGGGCC AGGGGGGTCT GCCGCCGGGC 
CAGCGGGTCC TGTACAAGCA GTCCATTGCG CAGCGCGCAC GGACTATGGC CCTGTACAAC 
CCCATCCCAG TCAAGCAGAA CTGCTTCACC GTCAACCGCT CGCTCTTCGT CTTCAGCGAG 
GACAACGTCG TCCGCAAATA CGCTAAGCGC ATCACCGAAT GGCCGCCCTT CGAATACATG 
ATCCTGGCCA CCATCATCGC CAACTGCATT GTTCTGGCCC TGGAGCAGCA CCTCCCTGAT 
GGGGACAAGA CTCCCATGTC TGAGCGACTA GATGACACGG AGCCTTACTT CATCGGGATC 
TTTTGCTTCG AGGCGGGCAT CAAGATCATA GCCCTGGGCT TTGTTTTCCA CAAGGGCTCC 
TACCTTCGGA ACGGCTGGAA TGTCATGGAC TTCGTGGTGG TCCTCACGGG GATTCTCGCC 
ACAGCTGGAA CTGACTTTGA CCTGCGCACA CTGAGGGCTG TGCGTGTGCT TAGGCCCCTG 
AAGCTGGTGT CTGGAATTCC AAGCTTGCAG GTGGTGCTTA AGTCCATCAT GAAGGCCATG 
GTCCCGCTGC TGCAGATTGG GCTGCTGCTC TTCTTTGCCA TCCTCATGTT TGCCATCATC 
GGCCTCGAAT TCTATATGGG CAAATTCCAT AAGGCCTGTT TCCCCAACAG CACAGATACA 
GAGCCTGTGG GTGACTTTCC CTGTGGCAAA GATCCCCCTG CTCGTCAGTG TGATGGTGAC 
ACCGAATGCC GGGAGTACTG GCCAGGACCC AACTTTGGCA TCACCAATTT TGACAACATC 
CTGTTTGCCA TCTTGACAGT GTTCCAGTGT ATCACCATGG AGGGCTGGAC TGACATCCTC 
TACAATACAA ATGATGCGGC TGGCAACACA TGGAACTGGT TGTACTTCAT CCCCCTCATC 
ATCATTGGCT CCTTCTTCAT GCTCAACCTG GTGCTGGGTG TGCTTTCCGG AGAGTTTGCC 
AAGGAGCGGG AGCGAGTCGA GAACCGCCGC GCCTTCCTGA AGCTCCGCAG GCAGCAGCAG 
ATTGAGCGAG AGCTGAATGG GTACTTGGAG TGGATCTTCA AGGCAGAGGA AGTCATGTTG 
GCAGAGGAGG ACAAGAATGC AGAAGAGAAA TCCCCTTTGG ATGCAGTGTT GAAGAGAGCT 
GCCACCAAGA AGAGCCGAAA TGACCTCATC CATGCAGAAG AGGGGGAGGA CCGGTTTGTA 
GACCTCTGTG CAGTTGGGTC TCCATTTGCT CGTGCCAGCC TCAAGAGTGG GAAGACGGAG 
AGCTCATCGT ACTTCCGGAG AAAGGAGAAG ATGTTCCGGT TCTTTATCCG GCGTATGGTG 
AAAGCACAGA GCTTCTACTG GGTGGTACTG TGTGTGGTGG CCCTGAACAC ACTGTGTGTG 
GCCATGGTGC ACTATAATCA GCCTCAGCGG CTTACCACTG CACTGTACTT TGCAGAGTTT 
GTTTTCCTGG GTCTCTTCCT CACAGAGATG TCCCTGAAGA TGTATGGCCT AGGGCCCAGA 
AGTTACTTCA GGTCTTCCTT CAACTGCTTT GACTTTGGGG TGATTGTGGG GAGTATCTTT 
GAAGTAGTCT GGGCTGCCAT CAAGCCAGGA ACCTCCTTTG GAATCAGTGT GCTGCGGGCT 
CTGCGACTGC TGAGGATATT CAAAGTTACC AAGTATTGGA ACTCTCTGAG GAACCTGGTG 
GTTTCCCTCC TCAATTCCAT GAAGTCCATC ATCAGCCTTC TCTTCCTGCT TTTCCTCTTC 
ATCGTGGTCT TCGCTCTGTT GGGGATGCAG CTGTTTGGGG GACAGTTCAA CTTTCAAGAT 
GAGACTCCAA CCACCAATTT TGATACCTTC CCAGCTGCCA TCCTCACTGT CTTTCAGATC 
CTGACAGGAG AGGATTGGAA TGCCGTAATG TATCATGGGA TTGAGTCGCA AGGTGGAGTC 
AGCAAAGGCA TGTTTTCTTC CTTTTACTTC ATCGTCCTGA CACTGTTTGG AAACTACACC 
CTGCTGAATG TTTTTCTGGC CATTGCTGTG GACAACCTTG CCAATGCCCA GGAGTTGACC 
AAGGATGAAG AGGAGATGGA AGAAGCAGCC AATCAGAAAC TTGCTCTTCA GAAGGCCAAA 
GAAGTAGCTG AAGTCAGCCC CATGTCTGCT GCCAATATCT CCATCGCTGC TTTTGTAAAG 
CAAACTCGAG GTACTGTATC TCGCAGCTCA TCTGTCTCCA GCGTAAACTC ACCCAGGCAG 
CAGAACTCGG CCAAGGCGCG CTCAGTATGG GAGCAGCGGG CCAGTCAGCT AAGGCTCCAG 
AATCTGCGTG CCAGCTGTGA GGCATTGTAC AGTGAGATGG ACCCTGAGGA GCGCCTGCGT 
TATGCCAGCA CGCGCCATGT GAGGCCAGAC ATGAAGACAC ACATGGACCG ACCCCTAGTG 
GTGGAGCCTG GTCGAGATGG CTTGCGGGGA CCCGTTGGGA GCAAGTCAAA GCCTGAAGGC 
ACGGAGGCCA CAGAAAGCGC GGACCTACCT CGCAGGCACC ACCGGCACCG TGATAGGGAC 
AAGACCTCAG CCACAGCACC TGCTGGAGGC GAACAGGACA GGACAGAAAG CACCGAGACC 
GGGGCCCGGG AGGAACGTGC GCGCCCTCGT CGAAGTCACA GCAAGGAGAC TCCAGGGGCT 
GACACGCAAG TGCGCTGTGA GCGCAGTAGA CGTCACCACC GGCGCGGCTC CCCGGAGGAG 
GCCACTGAAC GGGAGCCTCG GCGCCACCGT GCCCACCGGC ATGCACAGGA CTCAAGCAAG 
GAGGGCACGG CGCCGGTGCT TGTACCCAAG GGTGAGCGAC GAGCAAGACA CCGAGGCCCA 
CGCACGGGTC CACGTGAGGC AGAGAACAAC GAGGAGCCCA CACGCAGGCA CCGTGCAAGG 
CATAAGGTGC CACCCACACT GCAGCCCCCA GAGAGGGAGG CTGCAGAGAA GGAGAGCAAC 
GCGGTGGAAG GGGATAAGGA AACCCGAAAT CACCAGCCCA AGGAACCTCA CTGTGACCTG 
GAGGCCATTG CAGTTACAGG TGTGGGCCCT CTGCACATGC TGCCCAGCAC CTGTCTCCAG 
AAAGTGGACG AGCAACCAGA GGATGCAGAC AACCAGCGTA ATGTCACCCG GATGGGCAGT 
CAGCCCTCAG ATCCCAGCAC CACTGTGCAT GTCCCAGTGA CACTGACAGG CCCTCCTGGG 
GAGACCCCTG TAGTTCCCAG TGGTAACATG AACCTGGAAG GCCAAGCAGA GGGCAAGAAG 
GAGGCAGAGG CGGATGATGT GCTGAGAAGA GGCCCCAGGC CCATCGTTCC CTACAGCTCC 
ATGTTTTGTC TCAGCCCCAC CAACCTGCTT CGTCGCTTCT GCCATTACAT TGTGACCATG 
CGGTACTTTG AGATGGTAAT TCTTGTGGTC ATTGCCTTGA GCAGCATTGC CCTGGCTGCA 
GAGGATCCTG TGCGGACAGA TTCATTCAGG AACAACGCTT TAAAGTACAT GGATTACATC 
TTTACAGGAG TCTTCACCTT TGAAATGGTC ATAAAGATGA TAGACTTGGG CTTGCTGCTG 
CACCCTGGTG CCTACTTCCG GGACCTGTGG AACATTCTGG ACTTCATTGT TGTCAGTGGA 
GCCCTGGTGG CATTTGCGTT CTCGAGCTTC ATGGGAGGAT CCAAAGGGAA AGACATCAAT 
ACCATCAAGT CTCTGAGAGT CCTGCGTGTC CTGAGGCCCC TCAAGACCAT CAAGCGGCTG 
CCTAAGCTCA AGGCTGTCTT TGACTGTGTG GTGAACTCCC TGAAGAACGT CTTGAACATC 
CTGATTGTCT ACATGCTCTT CATGTTCATA TTTGCCGTCA TTGCCGTCCA GCTCTTCAAA 
GGGAAGTTCT TTTACTGTAC TGATGAATCC AAGGAGCTGG AGAGGGACTG CCGGGGTCAG 
TATTTGGATT ATGAGAAGGA AGAAGTAGAA GCCCAGCCAA GGCAGTGGAA GAAATATGAC 
TTCCACTATG ACAATGTTCT CTGGGCCTTG TTGACGCTGT TCACAGTGTC CACGGGAGAG 
GGGTGGCCCA TGGTGCTGAA ACACTCTGTG GATGCCACCT ATGAGGAACA GGGGCCCAGT 
CCCGGGTTCC GGATGGAGCT CTCCATCTTC TACGTGGTCT ACTTTGTGGT CTTCCCTTTT 
TTCTTTGTCA ACATCTTTGT GGCCTTGATC ATTATCACCT TCCAGGAACA GGGAGATAAG 
GTGATGTCTG AATGCAGCTT AGAAAAGAAT GAGAGGGCTT GCATTGATTT TGCCATCAGT 
GCCAAGCCCC TGACACGGTA CATGCCTCAA AACAAACAGT CGTTCCAGTA TAAGACATGG 
ACATTCGTGG TCTCTCCACC CTTTGAGTAC TTCATCATGG CTATGATAGC CCTCAACACA 
GTGGTGCTGA TGATGAAGTT CTATGATGCA CCTTATGAGT ACGAGCTGAT GCTGAAATGC 
CTGAACATTG TCTTCACATC CATGTTCTCG ATGGAGTGCA TACTGAAGAT CATCGCCTTT 
GGGGTATTGA ACTACTTCAG AGATGCCTGG AACGTCTTTG ACTTTGTCAC GGTTTTGGGA 
AGTATTACTG ATATTTTAGT AACAGAGATA GCGGAAACGA ACAACTTCAT CAACCTAAGC 
TTCCTTCGCC TCTTCCGGGC GGCACGGCTG ATCAAGCTGC TTCGCCAGGG CTACACCATC 
CGCATCCTAT TGTGGACCTT CGTCCAGTCC TTTAAGGCGC TGCCCTACGT GTGCCTCCTC 
ATTGCCATGC TGTTCTTCAT CTACGCCATC ATCGGCATGC AGGTTTTTGG AAACATTGCC 
CTTGATGATG ACACCAGTAT CAACCGACAC AACAACTTCC GGACATTTCT GCAAGCCTTA 
ATGCTATTGT TCAGGAGTGC CACTGGGGAG GCCTGGCATG AGATCATGCT GTCTTGTCTG 
GGCAACCGGG CCTGTGACCC ACATGCCAAC GCCAGTGAGT GCGGGAGCGA CTTTGCCTAT 
TTTTATTTTG TCTCCTTCAT CTTCCTCTGT TCCTTTCTGA TGTTGAACCT CTTTGTTGCT 
GTAATCATGG ACAATTTTGA GTACCTCACT CGGGACTCTT CCATCCTAGG GCCTCACCAC 
TTAGACGAAT TCATTCGAGT CTGGGCTGAA TACGACCCAG CTGCGTGTTG CCGGATTCAT 
TATAAGGATA TGTACAGTTT GTTGCGTTGT ATTGCGCCAC CCGTTGGCTT AGGAAAGAAC 
TGCCCTCGTA GGTTGGCCTA CAAGGTTTGC AACTTCAGAG CCTCCATCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cacna1b ( XM_006497640.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006497703.1 Mus musculus calcium channel, voltage-dependent, N type, alpha 1B subunit (Cacna1b), transcript variant X20, mRNA.

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CloneID	OMu29271
Clone ID Related Accession (Same CDS sequence)	XM_006497640.3
Accession Version	XM_006497640.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4956bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	voltage-dependent N-type calcium channel subunit alpha-1B isoform X18
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006497640.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTCCGCT TCGGGGACGA GCTAGGCGGC CGCTATGGGG GCACCGGCGG CGGGGAGCGG 
GCTCGGGGTG GCGGGGCCGG CGGGGCGGGT GGCCCGGGCC AGGGGGGTCT GCCGCCGGGC 
CAGCGGGTCC TGTACAAGCA GTCCATTGCG CAGCGCGCAC GGACTATGGC CCTGTACAAC 
CCCATCCCAG TCAAGCAGAA CTGCTTCACC GTCAACCGCT CGCTCTTCGT CTTCAGCGAG 
GACAACGTCG TCCGCAAATA CGCTAAGCGC ATCACCGAAT GGCCGCCCTT CGAATACATG 
ATCCTGGCCA CCATCATCGC CAACTGCATT GTTCTGGCCC TGGAGCAGCA CCTCCCTGAT 
GGGGACAAGA CTCCCATGTC TGAGCGACTA GATGACACGG AGCCTTACTT CATCGGGATC 
TTTTGCTTCG AGGCGGGCAT CAAGATCATA GCCCTGGGCT TTGTTTTCCA CAAGGGCTCC 
TACCTTCGGA ACGGCTGGAA TGTCATGGAC TTCGTGGTGG TCCTCACGGG GATTCTCGCC 
ACAGCTGGAA CTGACTTTGA CCTGCGCACA CTGAGGGCTG TGCGTGTGCT TAGGCCCCTG 
AAGCTGGTGT CTGGAATTCC AAGCTTGCAG GTGGTGCTTA AGTCCATCAT GAAGGCCATG 
GTCCCGCTGC TGCAGATTGG GCTGCTGCTC TTCTTTGCCA TCCTCATGTT TGCCATCATC 
GGCCTCGAAT TCTATATGGG CAAATTCCAT AAGGCCTGTT TCCCCAACAG CACAGATACA 
GAGCCTGTGG GTGACTTTCC CTGTGGCAAA GATCCCCCTG CTCGTCAGTG TGATGGTGAC 
ACCGAATGCC GGGAGTACTG GCCAGGACCC AACTTTGGCA TCACCAATTT TGACAACATC 
CTGTTTGCCA TCTTGACAGT GTTCCAGTGT ATCACCATGG AGGGCTGGAC TGACATCCTC 
TACAATACAA ATGATGCGGC TGGCAACACA TGGAACTGGT TGTACTTCAT CCCCCTCATC 
ATCATTGGCT CCTTCTTCAT GCTCAACCTG GTGCTGGGTG TGCTTTCCGG AGAGTTTGCC 
AAGGAGCGGG AGCGAGTCGA GAACCGCCGC GCCTTCCTGA AGCTCCGCAG GCAGCAGCAG 
ATTGAGCGAG AGCTGAATGG GTACTTGGAG TGGATCTTCA AGGCAGAGGA AGTCATGTTG 
GCAGAGGAGG ACAAGAATGC AGAAGAGAAA TCCCCTTTGG ATGCAGTGTT GAAGAGAGCT 
GCCACCAAGA AGAGCCGAAA TGACCTCATC CATGCAGAAG AGGGGGAGGA CCGGTTTGTA 
GACCTCTGTG CAGTTGGGTC TCCATTTGCT CGTGCCAGCC TCAAGAGTGG GAAGACGGAG 
AGCTCATCGT ACTTCCGGAG AAAGGAGAAG ATGTTCCGGT TCTTTATCCG GCGTATGGTG 
AAAGCACAGA GCTTCTACTG GGTGGTACTG TGTGTGGTGG CCCTGAACAC ACTGTGTGTG 
GCCATGGTGC ACTATAATCA GCCTCAGCGG CTTACCACTG CACTGTACTT TGCAGAGTTT 
GTTTTCCTGG GTCTCTTCCT CACAGAGATG TCCCTGAAGA TGTATGGCCT AGGGCCCAGA 
AGTTACTTCA GGTCTTCCTT CAACTGCTTT GACTTTGGGG TGATTGTGGG GAGTATCTTT 
GAAGTAGTCT GGGCTGCCAT CAAGCCAGGA ACCTCCTTTG GAATCAGTGT GCTGCGGGCT 
CTGCGACTGC TGAGGATATT CAAAGTTACC AAGTATTGGA ACTCTCTGAG GAACCTGGTG 
GTTTCCCTCC TCAATTCCAT GAAGTCCATC ATCAGCCTTC TCTTCCTGCT TTTCCTCTTC 
ATCGTGGTCT TCGCTCTGTT GGGGATGCAG CTGTTTGGGG GACAGTTCAA CTTTCAAGAT 
GAGACTCCAA CCACCAATTT TGATACCTTC CCAGCTGCCA TCCTCACTGT CTTTCAGATC 
CTGACAGGAG AGGATTGGAA TGCCGTAATG TATCATGGGA TTGAGTCGCA AGGTGGAGTC 
AGCAAAGGCA TGTTTTCTTC CTTTTACTTC ATCGTCCTGA CACTGTTTGG AAACTACACC 
CTGCTGAATG TTTTTCTGGC CATTGCTGTG GACAACCTTG CCAATGCCCA GGAGTTGACC 
AAGGATGAAG AGGAGATGGA AGAAGCAGCC AATCAGAAAC TTGCTCTTCA GAAGGCCAAA 
GAAGTAGCTG AAGTCAGCCC CATGTCTGCT GCCAATATCT CCATCGCTGC TTTTGTAAAG 
CAAACTCGAG GTACTGTATC TCGCAGCTCA TCTGTCTCCA GCGTAAACTC ACCCAGGCAG 
CAGAACTCGG CCAAGGCGCG CTCAGTATGG GAGCAGCGGG CCAGTCAGCT AAGGCTCCAG 
AATCTGCGTG CCAGCTGTGA GGCATTGTAC AGTGAGATGG ACCCTGAGGA GCGCCTGCGT 
TATGCCAGCA CGCGCCATGT GAGGCCAGAC ATGAAGACAC ACATGGACCG ACCCCTAGTG 
GTGGAGCCTG GTCGAGATGG CTTGCGGGGA CCCGTTGGGA GCAAGTCAAA GCCTGAAGGC 
ACGGAGGCCA CAGAAAGCGC GGACCTACCT CGCAGGCACC ACCGGCACCG TGATAGGGAC 
AAGACCTCAG CCACAGCACC TGCTGGAGGC GAACAGGACA GGACAGAAAG CACCGAGACC 
GGGGCCCGGG AGGAACGTGC GCGCCCTCGT CGAAGTCACA GCAAGGAGAC TCCAGGGGCT 
GACACGCAAG TGCGCTGTGA GCGCAGTAGA CGTCACCACC GGCGCGGCTC CCCGGAGGAG 
GCCACTGAAC GGGAGCCTCG GCGCCACCGT GCCCACCGGC ATGCACAGGA CTCAAGCAAG 
GAGGGCACGG CGCCGGTGCT TGTACCCAAG GGTGAGCGAC GAGCAAGACA CCGAGGCCCA 
CGCACGGGTC CACGTGAGGC AGAGAACAAC GAGGAGCCCA CACGCAGGCA CCGTGCAAGG 
CATAAGGTGC CACCCACACT GCAGCCCCCA GAGAGGGAGG CTGCAGAGAA GGAGAGCAAC 
GCGGTGGAAG GGGATAAGGA AACCCGAAAT CACCAGCCCA AGGAACCTCA CTGTGACCTG 
GAGGCCATTG CAGTTACAGG TGTGGGCCCT CTGCACATGC TGCCCAGCAC CTGTCTCCAG 
AAAGTGGACG AGCAACCAGA GGATGCAGAC AACCAGCGTA ATGTCACCCG GATGGGCAGT 
CAGCCCTCAG ATCCCAGCAC CACTGTGCAT GTCCCAGTGA CACTGACAGG CCCTCCTGGG 
GAGACCCCTG TAGTTCCCAG TGGTAACATG AACCTGGAAG GCCAAGCAGA GGGCAAGAAG 
GAGGCAGAGG CGGATGATGT GCTGAGAAGA GGCCCCAGGC CCATCGTTCC CTACAGCTCC 
ATGTTTTGTC TCAGCCCCAC CAACCTGCTT CGTCGCTTCT GCCATTACAT TGTGACCATG 
CGGTACTTTG AGATGGTAAT TCTTGTGGTC ATTGCCTTGA GCAGCATTGC CCTGGCTGCA 
GAGGATCCTG TGCGGACAGA TTCATTCAGG AACAACGCTT TAAAGTACAT GGATTACATC 
TTTACAGGAG TCTTCACCTT TGAAATGGTC ATAAAGATGA TAGACTTGGG CTTGCTGCTG 
CACCCTGGTG CCTACTTCCG GGACCTGTGG AACATTCTGG ACTTCATTGT TGTCAGTGGA 
GCCCTGGTGG CATTTGCGTT CTCGAGCTTC ATGGGAGGAT CCAAAGGGAA AGACATCAAT 
ACCATCAAGT CTCTGAGAGT CCTGCGTGTC CTGAGGCCCC TCAAGACCAT CAAGCGGCTG 
CCTAAGCTCA AGGCTGTCTT TGACTGTGTG GTGAACTCCC TGAAGAACGT CTTGAACATC 
CTGATTGTCT ACATGCTCTT CATGTTCATA TTTGCCGTCA TTGCCGTCCA GCTCTTCAAA 
GGGAAGTTCT TTTACTGTAC TGATGAATCC AAGGAGCTGG AGAGGGACTG CCGGGGTCAG 
TATTTGGATT ATGAGAAGGA AGAAGTAGAA GCCCAGCCAA GGCAGTGGAA GAAATATGAC 
TTCCACTATG ACAATGTTCT CTGGGCCTTG TTGACGCTGT TCACAGTGTC CACGGGAGAG 
GGGTGGCCCA TGGTGCTGAA ACACTCTGTG GATGCCACCT ATGAGGAACA GGGGCCCAGT 
CCCGGGTTCC GGATGGAGCT CTCCATCTTC TACGTGGTCT ACTTTGTGGT CTTCCCTTTT 
TTCTTTGTCA ACATCTTTGT GGCCTTGATC ATTATCACCT TCCAGGAACA GGGAGATAAG 
GTGATGTCTG AATGCAGCTT AGAAAAGAAT GAGAGGGCTT GCATTGATTT TGCCATCAGT 
GCCAAGCCCC TGACACGGTA CATGCCTCAA AACAAACAGT CGTTCCAGTA TAAGACATGG 
ACATTCGTGG TCTCTCCACC CTTTGAGTAC TTCATCATGG CTATGATAGC CCTCAACACA 
GTGGTGCTGA TGATGAAGTT CTATGATGCA CCTTATGAGT ACGAGCTGAT GCTGAAATGC 
CTGAACATTG TCTTCACATC CATGTTCTCG ATGGAGTGCA TACTGAAGAT CATCGCCTTT 
GGGGTATTGA ACTACTTCAG AGATGCCTGG AACGTCTTTG ACTTTGTCAC GGTTTTGGGA 
AGTATTACTG ATATTTTAGT AACAGAGATA GCGGAAACGA ACAACTTCAT CAACCTAAGC 
TTCCTTCGCC TCTTCCGGGC GGCACGGCTG ATCAAGCTGC TTCGCCAGGG CTACACCATC 
CGCATCCTAT TGTGGACCTT CGTCCAGTCC TTTAAGGCGC TGCCCTACGT GTGCCTCCTC 
ATTGCCATGC TGTTCTTCAT CTACGCCATC ATCGGCATGC AGGTGGGTGC CACCTGCCCA 
GACTGGATTG GACACAGGTC TCCTGTCGCT GGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006497703.1
CDS	154..5109
Translation	MVRFGDELGGRYGGTGGGERARGGGAGGAGGPGQGGLPPGQRVLYKQSIAQRARTMALYNPIPVKQNCFTVNRSLFVFSEDNVVRKYAKRITEWPPFEYMILATIIANCIVLALEQHLPDGDKTPMSERLDDTEPYFIGIFCFEAGIKIIALGFVFHKGSYLRNGWNVMDFVVVLTGILATAGTDFDLRTLRAVRVLRPLKLVSGIPSLQVVLKSIMKAMVPLLQIGLLLFFAILMFAIIGLEFYMGKFHKACFPNSTDTEPVGDFPCGKDPPARQCDGDTECREYWPGPNFGITNFDNILFAILTVFQCITMEGWTDILYNTNDAAGNTWNWLYFIPLIIIGSFFMLNLVLGVLSGEFAKERERVENRRAFLKLRRQQQIERELNGYLEWIFKAEEVMLAEEDKNAEEKSPLDAVLKRAATKKSRNDLIHAEEGEDRFVDLCAVGSPFARASLKSGKTESSSYFRRKEKMFRFFIRRMVKAQSFYWVVLCVVALNTLCVAMVHYNQPQRLTTALYFAEFVFLGLFLTEMSLKMYGLGPRSYFRSSFNCFDFGVIVGSIFEVVWAAIKPGTSFGISVLRALRLLRIFKVTKYWNSLRNLVVSLLNSMKSIISLLFLLFLFIVVFALLGMQLFGGQFNFQDETPTTNFDTFPAAILTVFQILTGEDWNAVMYHGIESQGGVSKGMFSSFYFIVLTLFGNYTLLNVFLAIAVDNLANAQELTKDEEEMEEAANQKLALQKAKEVAEVSPMSAANISIAAFVKQTRGTVSRSSSVSSVNSPRQQNSAKARSVWEQRASQLRLQNLRASCEALYSEMDPEERLRYASTRHVRPDMKTHMDRPLVVEPGRDGLRGPVGSKSKPEGTEATESADLPRRHHRHRDRDKTSATAPAGGEQDRTESTETGAREERARPRRSHSKETPGADTQVRCERSRRHHRRGSPEEATEREPRRHRAHRHAQDSSKEGTAPVLVPKGERRARHRGPRTGPREAENNEEPTRRHRARHKVPPTLQPPEREAAEKESNAVEGDKETRNHQPKEPHCDLEAIAVTGVGPLHMLPSTCLQKVDEQPEDADNQRNVTRMGSQPSDPSTTVHVPVTLTGPPGETPVVPSGNMNLEGQAEGKKEAEADDVLRRGPRPIVPYSSMFCLSPTNLLRRFCHYIVTMRYFEMVILVVIALSSIALAAEDPVRTDSFRNNALKYMDYIFTGVFTFEMVIKMIDLGLLLHPGAYFRDLWNILDFIVVSGALVAFAFSSFMGGSKGKDINTIKSLRVLRVLRPLKTIKRLPKLKAVFDCVVNSLKNVLNILIVYMLFMFIFAVIAVQLFKGKFFYCTDESKELERDCRGQYLDYEKEEVEAQPRQWKKYDFHYDNVLWALLTLFTVSTGEGWPMVLKHSVDATYEEQGPSPGFRMELSIFYVVYFVVFPFFFVNIFVALIIITFQEQGDKVMSECSLEKNERACIDFAISAKPLTRYMPQNKQSFQYKTWTFVVSPPFEYFIMAMIALNTVVLMMKFYDAPYEYELMLKCLNIVFTSMFSMECILKIIAFGVLNYFRDAWNVFDFVTVLGSITDILVTEIAETNNFINLSFLRLFRAARLIKLLRQGYTIRILLWTFVQSFKALPYVCLLIAMLFFIYAIIGMQVGATCPDWIGHRSPVAG

Target ORF information:

RefSeq Version	XM_006497640.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus calcium channel, voltage-dependent, N type, alpha 1B subunit (Cacna1b), transcript variant X20, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006497640.3

ORF Insert Sequence:

ATGGTCCGCT TCGGGGACGA GCTAGGCGGC CGCTATGGGG GCACCGGCGG CGGGGAGCGG 
GCTCGGGGTG GCGGGGCCGG CGGGGCGGGT GGCCCGGGCC AGGGGGGTCT GCCGCCGGGC 
CAGCGGGTCC TGTACAAGCA GTCCATTGCG CAGCGCGCAC GGACTATGGC CCTGTACAAC 
CCCATCCCAG TCAAGCAGAA CTGCTTCACC GTCAACCGCT CGCTCTTCGT CTTCAGCGAG 
GACAACGTCG TCCGCAAATA CGCTAAGCGC ATCACCGAAT GGCCGCCCTT CGAATACATG 
ATCCTGGCCA CCATCATCGC CAACTGCATT GTTCTGGCCC TGGAGCAGCA CCTCCCTGAT 
GGGGACAAGA CTCCCATGTC TGAGCGACTA GATGACACGG AGCCTTACTT CATCGGGATC 
TTTTGCTTCG AGGCGGGCAT CAAGATCATA GCCCTGGGCT TTGTTTTCCA CAAGGGCTCC 
TACCTTCGGA ACGGCTGGAA TGTCATGGAC TTCGTGGTGG TCCTCACGGG GATTCTCGCC 
ACAGCTGGAA CTGACTTTGA CCTGCGCACA CTGAGGGCTG TGCGTGTGCT TAGGCCCCTG 
AAGCTGGTGT CTGGAATTCC AAGCTTGCAG GTGGTGCTTA AGTCCATCAT GAAGGCCATG 
GTCCCGCTGC TGCAGATTGG GCTGCTGCTC TTCTTTGCCA TCCTCATGTT TGCCATCATC 
GGCCTCGAAT TCTATATGGG CAAATTCCAT AAGGCCTGTT TCCCCAACAG CACAGATACA 
GAGCCTGTGG GTGACTTTCC CTGTGGCAAA GATCCCCCTG CTCGTCAGTG TGATGGTGAC 
ACCGAATGCC GGGAGTACTG GCCAGGACCC AACTTTGGCA TCACCAATTT TGACAACATC 
CTGTTTGCCA TCTTGACAGT GTTCCAGTGT ATCACCATGG AGGGCTGGAC TGACATCCTC 
TACAATACAA ATGATGCGGC TGGCAACACA TGGAACTGGT TGTACTTCAT CCCCCTCATC 
ATCATTGGCT CCTTCTTCAT GCTCAACCTG GTGCTGGGTG TGCTTTCCGG AGAGTTTGCC 
AAGGAGCGGG AGCGAGTCGA GAACCGCCGC GCCTTCCTGA AGCTCCGCAG GCAGCAGCAG 
ATTGAGCGAG AGCTGAATGG GTACTTGGAG TGGATCTTCA AGGCAGAGGA AGTCATGTTG 
GCAGAGGAGG ACAAGAATGC AGAAGAGAAA TCCCCTTTGG ATGCAGTGTT GAAGAGAGCT 
GCCACCAAGA AGAGCCGAAA TGACCTCATC CATGCAGAAG AGGGGGAGGA CCGGTTTGTA 
GACCTCTGTG CAGTTGGGTC TCCATTTGCT CGTGCCAGCC TCAAGAGTGG GAAGACGGAG 
AGCTCATCGT ACTTCCGGAG AAAGGAGAAG ATGTTCCGGT TCTTTATCCG GCGTATGGTG 
AAAGCACAGA GCTTCTACTG GGTGGTACTG TGTGTGGTGG CCCTGAACAC ACTGTGTGTG 
GCCATGGTGC ACTATAATCA GCCTCAGCGG CTTACCACTG CACTGTACTT TGCAGAGTTT 
GTTTTCCTGG GTCTCTTCCT CACAGAGATG TCCCTGAAGA TGTATGGCCT AGGGCCCAGA 
AGTTACTTCA GGTCTTCCTT CAACTGCTTT GACTTTGGGG TGATTGTGGG GAGTATCTTT 
GAAGTAGTCT GGGCTGCCAT CAAGCCAGGA ACCTCCTTTG GAATCAGTGT GCTGCGGGCT 
CTGCGACTGC TGAGGATATT CAAAGTTACC AAGTATTGGA ACTCTCTGAG GAACCTGGTG 
GTTTCCCTCC TCAATTCCAT GAAGTCCATC ATCAGCCTTC TCTTCCTGCT TTTCCTCTTC 
ATCGTGGTCT TCGCTCTGTT GGGGATGCAG CTGTTTGGGG GACAGTTCAA CTTTCAAGAT 
GAGACTCCAA CCACCAATTT TGATACCTTC CCAGCTGCCA TCCTCACTGT CTTTCAGATC 
CTGACAGGAG AGGATTGGAA TGCCGTAATG TATCATGGGA TTGAGTCGCA AGGTGGAGTC 
AGCAAAGGCA TGTTTTCTTC CTTTTACTTC ATCGTCCTGA CACTGTTTGG AAACTACACC 
CTGCTGAATG TTTTTCTGGC CATTGCTGTG GACAACCTTG CCAATGCCCA GGAGTTGACC 
AAGGATGAAG AGGAGATGGA AGAAGCAGCC AATCAGAAAC TTGCTCTTCA GAAGGCCAAA 
GAAGTAGCTG AAGTCAGCCC CATGTCTGCT GCCAATATCT CCATCGCTGC TTTTGTAAAG 
CAAACTCGAG GTACTGTATC TCGCAGCTCA TCTGTCTCCA GCGTAAACTC ACCCAGGCAG 
CAGAACTCGG CCAAGGCGCG CTCAGTATGG GAGCAGCGGG CCAGTCAGCT AAGGCTCCAG 
AATCTGCGTG CCAGCTGTGA GGCATTGTAC AGTGAGATGG ACCCTGAGGA GCGCCTGCGT 
TATGCCAGCA CGCGCCATGT GAGGCCAGAC ATGAAGACAC ACATGGACCG ACCCCTAGTG 
GTGGAGCCTG GTCGAGATGG CTTGCGGGGA CCCGTTGGGA GCAAGTCAAA GCCTGAAGGC 
ACGGAGGCCA CAGAAAGCGC GGACCTACCT CGCAGGCACC ACCGGCACCG TGATAGGGAC 
AAGACCTCAG CCACAGCACC TGCTGGAGGC GAACAGGACA GGACAGAAAG CACCGAGACC 
GGGGCCCGGG AGGAACGTGC GCGCCCTCGT CGAAGTCACA GCAAGGAGAC TCCAGGGGCT 
GACACGCAAG TGCGCTGTGA GCGCAGTAGA CGTCACCACC GGCGCGGCTC CCCGGAGGAG 
GCCACTGAAC GGGAGCCTCG GCGCCACCGT GCCCACCGGC ATGCACAGGA CTCAAGCAAG 
GAGGGCACGG CGCCGGTGCT TGTACCCAAG GGTGAGCGAC GAGCAAGACA CCGAGGCCCA 
CGCACGGGTC CACGTGAGGC AGAGAACAAC GAGGAGCCCA CACGCAGGCA CCGTGCAAGG 
CATAAGGTGC CACCCACACT GCAGCCCCCA GAGAGGGAGG CTGCAGAGAA GGAGAGCAAC 
GCGGTGGAAG GGGATAAGGA AACCCGAAAT CACCAGCCCA AGGAACCTCA CTGTGACCTG 
GAGGCCATTG CAGTTACAGG TGTGGGCCCT CTGCACATGC TGCCCAGCAC CTGTCTCCAG 
AAAGTGGACG AGCAACCAGA GGATGCAGAC AACCAGCGTA ATGTCACCCG GATGGGCAGT 
CAGCCCTCAG ATCCCAGCAC CACTGTGCAT GTCCCAGTGA CACTGACAGG CCCTCCTGGG 
GAGACCCCTG TAGTTCCCAG TGGTAACATG AACCTGGAAG GCCAAGCAGA GGGCAAGAAG 
GAGGCAGAGG CGGATGATGT GCTGAGAAGA GGCCCCAGGC CCATCGTTCC CTACAGCTCC 
ATGTTTTGTC TCAGCCCCAC CAACCTGCTT CGTCGCTTCT GCCATTACAT TGTGACCATG 
CGGTACTTTG AGATGGTAAT TCTTGTGGTC ATTGCCTTGA GCAGCATTGC CCTGGCTGCA 
GAGGATCCTG TGCGGACAGA TTCATTCAGG AACAACGCTT TAAAGTACAT GGATTACATC 
TTTACAGGAG TCTTCACCTT TGAAATGGTC ATAAAGATGA TAGACTTGGG CTTGCTGCTG 
CACCCTGGTG CCTACTTCCG GGACCTGTGG AACATTCTGG ACTTCATTGT TGTCAGTGGA 
GCCCTGGTGG CATTTGCGTT CTCGAGCTTC ATGGGAGGAT CCAAAGGGAA AGACATCAAT 
ACCATCAAGT CTCTGAGAGT CCTGCGTGTC CTGAGGCCCC TCAAGACCAT CAAGCGGCTG 
CCTAAGCTCA AGGCTGTCTT TGACTGTGTG GTGAACTCCC TGAAGAACGT CTTGAACATC 
CTGATTGTCT ACATGCTCTT CATGTTCATA TTTGCCGTCA TTGCCGTCCA GCTCTTCAAA 
GGGAAGTTCT TTTACTGTAC TGATGAATCC AAGGAGCTGG AGAGGGACTG CCGGGGTCAG 
TATTTGGATT ATGAGAAGGA AGAAGTAGAA GCCCAGCCAA GGCAGTGGAA GAAATATGAC 
TTCCACTATG ACAATGTTCT CTGGGCCTTG TTGACGCTGT TCACAGTGTC CACGGGAGAG 
GGGTGGCCCA TGGTGCTGAA ACACTCTGTG GATGCCACCT ATGAGGAACA GGGGCCCAGT 
CCCGGGTTCC GGATGGAGCT CTCCATCTTC TACGTGGTCT ACTTTGTGGT CTTCCCTTTT 
TTCTTTGTCA ACATCTTTGT GGCCTTGATC ATTATCACCT TCCAGGAACA GGGAGATAAG 
GTGATGTCTG AATGCAGCTT AGAAAAGAAT GAGAGGGCTT GCATTGATTT TGCCATCAGT 
GCCAAGCCCC TGACACGGTA CATGCCTCAA AACAAACAGT CGTTCCAGTA TAAGACATGG 
ACATTCGTGG TCTCTCCACC CTTTGAGTAC TTCATCATGG CTATGATAGC CCTCAACACA 
GTGGTGCTGA TGATGAAGTT CTATGATGCA CCTTATGAGT ACGAGCTGAT GCTGAAATGC 
CTGAACATTG TCTTCACATC CATGTTCTCG ATGGAGTGCA TACTGAAGAT CATCGCCTTT 
GGGGTATTGA ACTACTTCAG AGATGCCTGG AACGTCTTTG ACTTTGTCAC GGTTTTGGGA 
AGTATTACTG ATATTTTAGT AACAGAGATA GCGGAAACGA ACAACTTCAT CAACCTAAGC 
TTCCTTCGCC TCTTCCGGGC GGCACGGCTG ATCAAGCTGC TTCGCCAGGG CTACACCATC 
CGCATCCTAT TGTGGACCTT CGTCCAGTCC TTTAAGGCGC TGCCCTACGT GTGCCTCCTC 
ATTGCCATGC TGTTCTTCAT CTACGCCATC ATCGGCATGC AGGTGGGTGC CACCTGCCCA 
GACTGGATTG GACACAGGTC TCCTGTCGCT GGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cacna1b ( XM_017315140.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017170629.1 Mus musculus calcium channel, voltage-dependent, N type, alpha 1B subunit (Cacna1b), transcript variant X3, mRNA.

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CloneID	OMu80051
Clone ID Related Accession (Same CDS sequence)	XM_017315140.1
Accession Version	XM_017315140.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7053bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	voltage-dependent N-type calcium channel subunit alpha-1B isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTCCGCT TCGGGGACGA GCTAGGCGGC CGCTATGGGG GCACCGGCGG CGGGGAGCGG 
GCTCGGGGTG GCGGGGCCGG CGGGGCGGGT GGCCCGGGCC AGGGGGGTCT GCCGCCGGGC 
CAGCGGGTCC TGTACAAGCA GTCCATTGCG CAGCGCGCAC GGACTATGGC CCTGTACAAC 
CCCATCCCAG TCAAGCAGAA CTGCTTCACC GTCAACCGCT CGCTCTTCGT CTTCAGCGAG 
GACAACGTCG TCCGCAAATA CGCTAAGCGC ATCACCGAAT GGCCGCCCTT CGAATACATG 
ATCCTGGCCA CCATCATCGC CAACTGCATT GTTCTGGCCC TGGAGCAGCA CCTCCCTGAT 
GGGGACAAGA CTCCCATGTC TGAGCGACTA GATGACACGG AGCCTTACTT CATCGGGATC 
TTTTGCTTCG AGGCGGGCAT CAAGATCATA GCCCTGGGCT TTGTTTTCCA CAAGGGCTCC 
TACCTTCGGA ACGGCTGGAA TGTCATGGAC TTCGTGGTGG TCCTCACGGG GATTCTCGCC 
ACAGCTGGAA CTGACTTTGA CCTGCGCACA CTGAGGGCTG TGCGTGTGCT TAGGCCCCTG 
AAGCTGGTGT CTGGAATTCC AAGCTTGCAG GTGGTGCTTA AGTCCATCAT GAAGGCCATG 
GTCCCGCTGC TGCAGATTGG GCTGCTGCTC TTCTTTGCCA TCCTCATGTT TGCCATCATC 
GGCCTCGAAT TCTATATGGG CAAATTCCAT AAGGCCTGTT TCCCCAACAG CACAGATACA 
GAGCCTGTGG GTGACTTTCC CTGTGGCAAA GATCCCCCTG CTCGTCAGTG TGATGGTGAC 
ACCGAATGCC GGGAGTACTG GCCAGGACCC AACTTTGGCA TCACCAATTT TGACAACATC 
CTGTTTGCCA TCTTGACAGT GTTCCAGTGT ATCACCATGG AGGGCTGGAC TGACATCCTC 
TACAATACAA ATGATGCGGC TGGCAACACA TGGAACTGGT TGTACTTCAT CCCCCTCATC 
ATCATTGGCT CCTTCTTCAT GCTCAACCTG GTGCTGGGTG TGCTTTCCGG AGAGTTTGCC 
AAGGAGCGGG AGCGAGTCGA GAACCGCCGC GCCTTCCTGA AGCTCCGCAG GCAGCAGCAG 
ATTGAGCGAG AGCTGAATGG GTACTTGGAG TGGATCTTCA AGGCAGAGGA AGTCATGTTG 
GCAGAGGAGG ACAAGAATGC AGAAGAGAAA TCCCCTTTGG ATGTGTTGAA GAGAGCTGCC 
ACCAAGAAGA GCCGAAATGA CCTCATCCAT GCAGAAGAGG GGGAGGACCG GTTTGTAGAC 
CTCTGTGCAG TTGGGTCTCC ATTTGCTCGT GCCAGCCTCA AGAGTGGGAA GACGGAGAGC 
TCATCGTACT TCCGGAGAAA GGAGAAGATG TTCCGGTTCT TTATCCGGCG TATGGTGAAA 
GCACAGAGCT TCTACTGGGT GGTACTGTGT GTGGTGGCCC TGAACACACT GTGTGTGGCC 
ATGGTGCACT ATAATCAGCC TCAGCGGCTT ACCACTGCAC TGTACTTTGC AGAGTTTGTT 
TTCCTGGGTC TCTTCCTCAC AGAGATGTCC CTGAAGATGT ATGGCCTAGG GCCCAGAAGT 
TACTTCAGGT CTTCCTTCAA CTGCTTTGAC TTTGGGGTGA TTGTGGGGAG TATCTTTGAA 
GTAGTCTGGG CTGCCATCAA GCCAGGAACC TCCTTTGGAA TCAGTGTGCT GCGGGCTCTG 
CGACTGCTGA GGATATTCAA AGTTACCAAG TATTGGAACT CTCTGAGGAA CCTGGTGGTT 
TCCCTCCTCA ATTCCATGAA GTCCATCATC AGCCTTCTCT TCCTGCTTTT CCTCTTCATC 
GTGGTCTTCG CTCTGTTGGG GATGCAGCTG TTTGGGGGAC AGTTCAACTT TCAAGATGAG 
ACTCCAACCA CCAATTTTGA TACCTTCCCA GCTGCCATCC TCACTGTCTT TCAGATCCTG 
ACAGGAGAGG ATTGGAATGC CGTAATGTAT CATGGGATTG AGTCGCAAGG TGGAGTCAGC 
AAAGGCATGT TTTCTTCCTT TTACTTCATC GTCCTGACAC TGTTTGGAAA CTACACCCTG 
CTGAATGTTT TTCTGGCCAT TGCTGTGGAC AACCTTGCCA ATGCCCAGGA GTTGACCAAG 
GATGAAGAGG AGATGGAAGA AGCAGCCAAT CAGAAACTTG CTCTTCAGAA GGCCAAAGAA 
GTAGCTGAAG TCAGCCCCAT GTCTGCTGCC AATATCTCCA TCGCTGCTTT TGTAAAGCAA 
ACTCGAGGTA CTGTATCTCG CAGCTCATCT GTCTCCAGCG TAAACTCACC CAGGCAGCAG 
AACTCGGCCA AGGCGCGCTC AGTATGGGAG CAGCGGGCCA GTCAGCTAAG GCTCCAGAAT 
CTGCGTGCCA GCTGTGAGGC ATTGTACAGT GAGATGGACC CTGAGGAGCG CCTGCGTTAT 
GCCAGCACGC GCCATGTGAG GCCAGACATG AAGACACACA TGGACCGACC CCTAGTGGTG 
GAGCCTGGTC GAGATGGCTT GCGGGGACCC GTTGGGAGCA AGTCAAAGCC TGAAGGCACG 
GAGGCCACAG AAAGCGCGGA CCTACCTCGC AGGCACCACC GGCACCGTGA TAGGGACAAG 
ACCTCAGCCA CAGCACCTGC TGGAGGCGAA CAGGACAGGA CAGAAAGCAC CGAGACCGGG 
GCCCGGGAGG AACGTGCGCG CCCTCGTCGA AGTCACAGCA AGGAGACTCC AGGGGCTGAC 
ACGCAAGTGC GCTGTGAGCG CAGTAGACGT CACCACCGGC GCGGCTCCCC GGAGGAGGCC 
ACTGAACGGG AGCCTCGGCG CCACCGTGCC CACCGGCATG CACAGGACTC AAGCAAGGAG 
GGCACGGCGC CGGTGCTTGT ACCCAAGGGT GAGCGACGAG CAAGACACCG AGGCCCACGC 
ACGGGTCCAC GTGAGGCAGA GAACAACGAG GAGCCCACAC GCAGGCACCG TGCAAGGCAT 
AAGGTGCCAC CCACACTGCA GCCCCCAGAG AGGGAGGCTG CAGAGAAGGA GAGCAACGCG 
GTGGAAGGGG ATAAGGAAAC CCGAAATCAC CAGCCCAAGG AACCTCACTG TGACCTGGAG 
GCCATTGCAG TTACAGGTGT GGGCCCTCTG CACATGCTGC CCAGCACCTG TCTCCAGAAA 
GTGGACGAGC AACCAGAGGA TGCAGACAAC CAGCGTAATG TCACCCGGAT GGGCAGTCAG 
CCCTCAGATC CCAGCACCAC TGTGCATGTC CCAGTGACAC TGACAGGCCC TCCTGGGGAG 
ACCCCTGTAG TTCCCAGTGG TAACATGAAC CTGGAAGGCC AAGCAGAGGG CAAGAAGGAG 
GCAGAGGCGG ATGATGTGCT GAGAAGAGGC CCCAGGCCCA TCGTTCCCTA CAGCTCCATG 
TTTTGTCTCA GCCCCACCAA CCTGCTTCGT CGCTTCTGCC ATTACATTGT GACCATGCGG 
TACTTTGAGA TGGTAATTCT TGTGGTCATT GCCTTGAGCA GCATTGCCCT GGCTGCAGAG 
GATCCTGTGC GGACAGATTC ATTCAGGAAC AACGCTTTAA AGTACATGGA TTACATCTTT 
ACAGGAGTCT TCACCTTTGA AATGGTCATA AAGATGATAG ACTTGGGCTT GCTGCTGCAC 
CCTGGTGCCT ACTTCCGGGA CCTGTGGAAC ATTCTGGACT TCATTGTTGT CAGTGGAGCC 
CTGGTGGCAT TTGCGTTCTC GAGCTTCATG GGAGGATCCA AAGGGAAAGA CATCAATACC 
ATCAAGTCTC TGAGAGTCCT GCGTGTCCTG AGGCCCCTCA AGACCATCAA GCGGCTGCCT 
AAGCTCAAGG CTGTCTTTGA CTGTGTGGTG AACTCCCTGA AGAACGTCTT GAACATCCTG 
ATTGTCTACA TGCTCTTCAT GTTCATATTT GCCGTCATTG CCGTCCAGCT CTTCAAAGGG 
AAGTTCTTTT ACTGTACTGA TGAATCCAAG GAGCTGGAGA GGGACTGCCG GGGTCAGTAT 
TTGGATTATG AGAAGGAAGA AGTAGAAGCC CAGCCAAGGC AGTGGAAGAA ATATGACTTC 
CACTATGACA ATGTTCTCTG GGCCTTGTTG ACGCTGTTCA CAGTGTCCAC GGGAGAGGGG 
TGGCCCATGG TGCTGAAACA CTCTGTGGAT GCCACCTATG AGGAACAGGG GCCCAGTCCC 
GGGTTCCGGA TGGAGCTCTC CATCTTCTAC GTGGTCTACT TTGTGGTCTT CCCTTTTTTC 
TTTGTCAACA TCTTTGTGGC CTTGATCATT ATCACCTTCC AGGAACAGGG AGATAAGGTG 
ATGTCTGAAT GCAGCTTAGA AAAGAATGAG AGGGCTTGCA TTGATTTTGC CATCAGTGCC 
AAGCCCCTGA CACGGTACAT GCCTCAAAAC AAACAGTCGT TCCAGTATAA GACATGGACA 
TTCGTGGTCT CTCCACCCTT TGAGTACTTC ATCATGGCTA TGATAGCCCT CAACACAGTG 
GTGCTGATGA TGAAGTTCTA TGATGCACCT TATGAGTACG AGCTGATGCT GAAATGCCTG 
AACATTGTCT TCACATCCAT GTTCTCGATG GAGTGCATAC TGAAGATCAT CGCCTTTGGG 
GTATTGAACT ACTTCAGAGA TGCCTGGAAC GTCTTTGACT TTGTCACGGT TTTGGGAAGT 
ATTACTGATA TTTTAGTAAC AGAGATAGCG GAAACGAACA ACTTCATCAA CCTAAGCTTC 
CTTCGCCTCT TCCGGGCGGC ACGGCTGATC AAGCTGCTTC GCCAGGGCTA CACCATCCGC 
ATCCTATTGT GGACCTTCGT CCAGTCCTTT AAGGCGCTGC CCTACGTGTG CCTCCTCATT 
GCCATGCTGT TCTTCATCTA CGCCATCATC GGCATGCAGG TTTTTGGAAA CATTGCCCTT 
GATGATGACA CCAGTATCAA CCGACACAAC AACTTCCGGA CATTTCTGCA AGCCTTAATG 
CTATTGTTCA GGAGTGCCAC TGGGGAGGCC TGGCATGAGA TCATGCTGTC TTGTCTGGGC 
AACCGGGCCT GTGACCCACA TGCCAACGCC AGTGAGTGCG GGAGCGACTT TGCCTATTTT 
TATTTTGTCT CCTTCATCTT CCTCTGTTCC TTTCTGATGT TGAACCTCTT TGTTGCTGTA 
ATCATGGACA ATTTTGAGTA CCTCACTCGG GACTCTTCCA TCCTAGGGCC TCACCACTTA 
GACGAATTCA TTCGAGTCTG GGCTGAATAC GACCCAGCTG CGTGTGGGCG CATCAGTTAC 
AATGACATGT TTGAGATGCT GAAACACATG TCCCCACCTC TGGGGTTGGG GAAGAAATGC 
CCGGCTCGAG TTGCATACAA GCGCCTGGTT CGCATGAACA TGCCCATATC CAATGAGGAC 
ATGACGGTGC ACTTTACGTC CACACTGATG GCCCTCATCC GGACAGCACT GGAGATCAAG 
CTTGCCCCAG CGGGGACGAA GCAGCACCAG TGTGATGCTG AGCTGAGAAA AGAGATCTCT 
TCTGTGTGGG CTAATCTGCC CCAGAAGACT CTGGACTTAC TGGTACCACC CCACAAACCT 
GACGAGATGA CAGTGGGGAA GGTCTATGCT GCTCTCATGA TATTTGACTT CTACAAACAG 
AACAAAACCA CCAGAGATCA GACTCACCAA GCTCCCGGAG GCCTGTCCCA GATGGGTCCC 
GTTTCCCTGT TCCACCCTCT GAAGGCCACC CTGGAACAGA CACAGCCCGC TGTGCTTCGA 
GGAGCTCGGG TTTTCCTTCG GCAAAAGAGT GCAACTTCCC TCAGCAATGG GGGTGCCATA 
CAAACCCAGG AAAGTGGCAT CAAGGAGTCG CTGTCCTGGG GCACGCAGAG GACCCAAGAT 
GCACTTTATG AGGCCAGAGC ACCTCTAGAA CGTGGCCATT CTGCAGAGAT CCCTGTGGGG 
CAGTCAGGAA CACTGGCTGT GGATGTCCAG ATGCAGAACA TGACACTGAG AGGACCAGAT 
GGGGAGCCCC AGCCTGGGCT GGAAAGCCAA GGCAGAGCTG CCTCTATGCC ACGCCTAGCG 
GCAGAAACAC AGCCGGCCCC TAATGCCAGC CCCATGAAGC GCTCCATCTC CACACTGGCT 
CCACGCCCAC ATGGGACTCA GCTTTGCAGC ACAGTTCTGG ACCGGCCTCC TCCTAGCCAG 
GCATCACATC ACCACCACCA CCGCTGCCAC CGGCGCAGAG ACAAGAAGCA AAGGTCCCTG 
GAAAAGGGGC CCAGCCTGTC TGTTGACCCA GAAGGTGCAC CAAGCACTGC TGCAGGACCT 
GGTCTGCCCC ATGGAGAAGG ATCCACCGCC TGCCGGCGGG ACCGTAAACA GGAGCGAGGC 
CGGTCCCAGG AGCGGAGGCA GCCCTCATCT TCCTCTTCAG AGAAGCAGCG CTTCTATTCC 
TGTGACCGCT TTGGGAGCCG GGAGCCCCCG CAACTGATGC CCTCACTCAG TAGCCACCCC 
ACATCGCCAA CAGCGGCGCT AGAGCCAGCA CCCCACCCAC AGGGCAGTGG TTCCGTTAAT 
GGGAGCCCCT TGATGTCAAC ATCCGGTGCT AGCACCCCGG GCCGAGGTGG GCGGAGGCAG 
CTCCCCCAGA CTCCTCTGAC CCCACGCCCC AGCATCACCT ACAAGACCGC CAATTCCTCG 
CCTGTCCACT TTGCTGAGGG TCAGAGCGGC CTCCCAGCCT TCTCCCCTGG CCGTCTCAGC 
CGCGGCCTTT CTGAACACAA TGCCCTGCTC CAGAAAGAGC CCCTGAGCCA GCCTCTAGCT 
CCTGGCTCCC GAATTGGCTC TGACCCTTAC CTAGGGCAGC GTCTGGACAG TGAGGCCTCC 
GCCCACACCC TGCCTGAGGA TACACTCACC TTTGAAGAGG CAGTGGCCAC CAACTCTGGC 
CGCTCCTCCA GGACTTCCTA TGTGTCCTCC CTCACTTCCC AATCCCACCC TCTCCGCCGT 
GTACCCAATG GCTATCACTG CACTTTGGGA CTCAGCACTG GCGTCCGGGC GCGGCACAGC 
TACCACCACC CCGATCAGGA CCACTGGTGC TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017170629.1
CDS	146..7198
Translation	MVRFGDELGGRYGGTGGGERARGGGAGGAGGPGQGGLPPGQRVLYKQSIAQRARTMALYNPIPVKQNCFTVNRSLFVFSEDNVVRKYAKRITEWPPFEYMILATIIANCIVLALEQHLPDGDKTPMSERLDDTEPYFIGIFCFEAGIKIIALGFVFHKGSYLRNGWNVMDFVVVLTGILATAGTDFDLRTLRAVRVLRPLKLVSGIPSLQVVLKSIMKAMVPLLQIGLLLFFAILMFAIIGLEFYMGKFHKACFPNSTDTEPVGDFPCGKDPPARQCDGDTECREYWPGPNFGITNFDNILFAILTVFQCITMEGWTDILYNTNDAAGNTWNWLYFIPLIIIGSFFMLNLVLGVLSGEFAKERERVENRRAFLKLRRQQQIERELNGYLEWIFKAEEVMLAEEDKNAEEKSPLDVLKRAATKKSRNDLIHAEEGEDRFVDLCAVGSPFARASLKSGKTESSSYFRRKEKMFRFFIRRMVKAQSFYWVVLCVVALNTLCVAMVHYNQPQRLTTALYFAEFVFLGLFLTEMSLKMYGLGPRSYFRSSFNCFDFGVIVGSIFEVVWAAIKPGTSFGISVLRALRLLRIFKVTKYWNSLRNLVVSLLNSMKSIISLLFLLFLFIVVFALLGMQLFGGQFNFQDETPTTNFDTFPAAILTVFQILTGEDWNAVMYHGIESQGGVSKGMFSSFYFIVLTLFGNYTLLNVFLAIAVDNLANAQELTKDEEEMEEAANQKLALQKAKEVAEVSPMSAANISIAAFVKQTRGTVSRSSSVSSVNSPRQQNSAKARSVWEQRASQLRLQNLRASCEALYSEMDPEERLRYASTRHVRPDMKTHMDRPLVVEPGRDGLRGPVGSKSKPEGTEATESADLPRRHHRHRDRDKTSATAPAGGEQDRTESTETGAREERARPRRSHSKETPGADTQVRCERSRRHHRRGSPEEATEREPRRHRAHRHAQDSSKEGTAPVLVPKGERRARHRGPRTGPREAENNEEPTRRHRARHKVPPTLQPPEREAAEKESNAVEGDKETRNHQPKEPHCDLEAIAVTGVGPLHMLPSTCLQKVDEQPEDADNQRNVTRMGSQPSDPSTTVHVPVTLTGPPGETPVVPSGNMNLEGQAEGKKEAEADDVLRRGPRPIVPYSSMFCLSPTNLLRRFCHYIVTMRYFEMVILVVIALSSIALAAEDPVRTDSFRNNALKYMDYIFTGVFTFEMVIKMIDLGLLLHPGAYFRDLWNILDFIVVSGALVAFAFSSFMGGSKGKDINTIKSLRVLRVLRPLKTIKRLPKLKAVFDCVVNSLKNVLNILIVYMLFMFIFAVIAVQLFKGKFFYCTDESKELERDCRGQYLDYEKEEVEAQPRQWKKYDFHYDNVLWALLTLFTVSTGEGWPMVLKHSVDATYEEQGPSPGFRMELSIFYVVYFVVFPFFFVNIFVALIIITFQEQGDKVMSECSLEKNERACIDFAISAKPLTRYMPQNKQSFQYKTWTFVVSPPFEYFIMAMIALNTVVLMMKFYDAPYEYELMLKCLNIVFTSMFSMECILKIIAFGVLNYFRDAWNVFDFVTVLGSITDILVTEIAETNNFINLSFLRLFRAARLIKLLRQGYTIRILLWTFVQSFKALPYVCLLIAMLFFIYAIIGMQVFGNIALDDDTSINRHNNFRTFLQALMLLFRSATGEAWHEIMLSCLGNRACDPHANASECGSDFAYFYFVSFIFLCSFLMLNLFVAVIMDNFEYLTRDSSILGPHHLDEFIRVWAEYDPAACGRISYNDMFEMLKHMSPPLGLGKKCPARVAYKRLVRMNMPISNEDMTVHFTSTLMALIRTALEIKLAPAGTKQHQCDAELRKEISSVWANLPQKTLDLLVPPHKPDEMTVGKVYAALMIFDFYKQNKTTRDQTHQAPGGLSQMGPVSLFHPLKATLEQTQPAVLRGARVFLRQKSATSLSNGGAIQTQESGIKESLSWGTQRTQDALYEARAPLERGHSAEIPVGQSGTLAVDVQMQNMTLRGPDGEPQPGLESQGRAASMPRLAAETQPAPNASPMKRSISTLAPRPHGTQLCSTVLDRPPPSQASHHHHHRCHRRRDKKQRSLEKGPSLSVDPEGAPSTAAGPGLPHGEGSTACRRDRKQERGRSQERRQPSSSSSEKQRFYSCDRFGSREPPQLMPSLSSHPTSPTAALEPAPHPQGSGSVNGSPLMSTSGASTPGRGGRRQLPQTPLTPRPSITYKTANSSPVHFAEGQSGLPAFSPGRLSRGLSEHNALLQKEPLSQPLAPGSRIGSDPYLGQRLDSEASAHTLPEDTLTFEEAVATNSGRSSRTSYVSSLTSQSHPLRRVPNGYHCTLGLSTGVRARHSYHHPDQDHWC

Target ORF information:

RefSeq Version	XM_017315140.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus calcium channel, voltage-dependent, N type, alpha 1B subunit (Cacna1b), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017315140.1

ORF Insert Sequence:

ATGGTCCGCT TCGGGGACGA GCTAGGCGGC CGCTATGGGG GCACCGGCGG CGGGGAGCGG 
GCTCGGGGTG GCGGGGCCGG CGGGGCGGGT GGCCCGGGCC AGGGGGGTCT GCCGCCGGGC 
CAGCGGGTCC TGTACAAGCA GTCCATTGCG CAGCGCGCAC GGACTATGGC CCTGTACAAC 
CCCATCCCAG TCAAGCAGAA CTGCTTCACC GTCAACCGCT CGCTCTTCGT CTTCAGCGAG 
GACAACGTCG TCCGCAAATA CGCTAAGCGC ATCACCGAAT GGCCGCCCTT CGAATACATG 
ATCCTGGCCA CCATCATCGC CAACTGCATT GTTCTGGCCC TGGAGCAGCA CCTCCCTGAT 
GGGGACAAGA CTCCCATGTC TGAGCGACTA GATGACACGG AGCCTTACTT CATCGGGATC 
TTTTGCTTCG AGGCGGGCAT CAAGATCATA GCCCTGGGCT TTGTTTTCCA CAAGGGCTCC 
TACCTTCGGA ACGGCTGGAA TGTCATGGAC TTCGTGGTGG TCCTCACGGG GATTCTCGCC 
ACAGCTGGAA CTGACTTTGA CCTGCGCACA CTGAGGGCTG TGCGTGTGCT TAGGCCCCTG 
AAGCTGGTGT CTGGAATTCC AAGCTTGCAG GTGGTGCTTA AGTCCATCAT GAAGGCCATG 
GTCCCGCTGC TGCAGATTGG GCTGCTGCTC TTCTTTGCCA TCCTCATGTT TGCCATCATC 
GGCCTCGAAT TCTATATGGG CAAATTCCAT AAGGCCTGTT TCCCCAACAG CACAGATACA 
GAGCCTGTGG GTGACTTTCC CTGTGGCAAA GATCCCCCTG CTCGTCAGTG TGATGGTGAC 
ACCGAATGCC GGGAGTACTG GCCAGGACCC AACTTTGGCA TCACCAATTT TGACAACATC 
CTGTTTGCCA TCTTGACAGT GTTCCAGTGT ATCACCATGG AGGGCTGGAC TGACATCCTC 
TACAATACAA ATGATGCGGC TGGCAACACA TGGAACTGGT TGTACTTCAT CCCCCTCATC 
ATCATTGGCT CCTTCTTCAT GCTCAACCTG GTGCTGGGTG TGCTTTCCGG AGAGTTTGCC 
AAGGAGCGGG AGCGAGTCGA GAACCGCCGC GCCTTCCTGA AGCTCCGCAG GCAGCAGCAG 
ATTGAGCGAG AGCTGAATGG GTACTTGGAG TGGATCTTCA AGGCAGAGGA AGTCATGTTG 
GCAGAGGAGG ACAAGAATGC AGAAGAGAAA TCCCCTTTGG ATGTGTTGAA GAGAGCTGCC 
ACCAAGAAGA GCCGAAATGA CCTCATCCAT GCAGAAGAGG GGGAGGACCG GTTTGTAGAC 
CTCTGTGCAG TTGGGTCTCC ATTTGCTCGT GCCAGCCTCA AGAGTGGGAA GACGGAGAGC 
TCATCGTACT TCCGGAGAAA GGAGAAGATG TTCCGGTTCT TTATCCGGCG TATGGTGAAA 
GCACAGAGCT TCTACTGGGT GGTACTGTGT GTGGTGGCCC TGAACACACT GTGTGTGGCC 
ATGGTGCACT ATAATCAGCC TCAGCGGCTT ACCACTGCAC TGTACTTTGC AGAGTTTGTT 
TTCCTGGGTC TCTTCCTCAC AGAGATGTCC CTGAAGATGT ATGGCCTAGG GCCCAGAAGT 
TACTTCAGGT CTTCCTTCAA CTGCTTTGAC TTTGGGGTGA TTGTGGGGAG TATCTTTGAA 
GTAGTCTGGG CTGCCATCAA GCCAGGAACC TCCTTTGGAA TCAGTGTGCT GCGGGCTCTG 
CGACTGCTGA GGATATTCAA AGTTACCAAG TATTGGAACT CTCTGAGGAA CCTGGTGGTT 
TCCCTCCTCA ATTCCATGAA GTCCATCATC AGCCTTCTCT TCCTGCTTTT CCTCTTCATC 
GTGGTCTTCG CTCTGTTGGG GATGCAGCTG TTTGGGGGAC AGTTCAACTT TCAAGATGAG 
ACTCCAACCA CCAATTTTGA TACCTTCCCA GCTGCCATCC TCACTGTCTT TCAGATCCTG 
ACAGGAGAGG ATTGGAATGC CGTAATGTAT CATGGGATTG AGTCGCAAGG TGGAGTCAGC 
AAAGGCATGT TTTCTTCCTT TTACTTCATC GTCCTGACAC TGTTTGGAAA CTACACCCTG 
CTGAATGTTT TTCTGGCCAT TGCTGTGGAC AACCTTGCCA ATGCCCAGGA GTTGACCAAG 
GATGAAGAGG AGATGGAAGA AGCAGCCAAT CAGAAACTTG CTCTTCAGAA GGCCAAAGAA 
GTAGCTGAAG TCAGCCCCAT GTCTGCTGCC AATATCTCCA TCGCTGCTTT TGTAAAGCAA 
ACTCGAGGTA CTGTATCTCG CAGCTCATCT GTCTCCAGCG TAAACTCACC CAGGCAGCAG 
AACTCGGCCA AGGCGCGCTC AGTATGGGAG CAGCGGGCCA GTCAGCTAAG GCTCCAGAAT 
CTGCGTGCCA GCTGTGAGGC ATTGTACAGT GAGATGGACC CTGAGGAGCG CCTGCGTTAT 
GCCAGCACGC GCCATGTGAG GCCAGACATG AAGACACACA TGGACCGACC CCTAGTGGTG 
GAGCCTGGTC GAGATGGCTT GCGGGGACCC GTTGGGAGCA AGTCAAAGCC TGAAGGCACG 
GAGGCCACAG AAAGCGCGGA CCTACCTCGC AGGCACCACC GGCACCGTGA TAGGGACAAG 
ACCTCAGCCA CAGCACCTGC TGGAGGCGAA CAGGACAGGA CAGAAAGCAC CGAGACCGGG 
GCCCGGGAGG AACGTGCGCG CCCTCGTCGA AGTCACAGCA AGGAGACTCC AGGGGCTGAC 
ACGCAAGTGC GCTGTGAGCG CAGTAGACGT CACCACCGGC GCGGCTCCCC GGAGGAGGCC 
ACTGAACGGG AGCCTCGGCG CCACCGTGCC CACCGGCATG CACAGGACTC AAGCAAGGAG 
GGCACGGCGC CGGTGCTTGT ACCCAAGGGT GAGCGACGAG CAAGACACCG AGGCCCACGC 
ACGGGTCCAC GTGAGGCAGA GAACAACGAG GAGCCCACAC GCAGGCACCG TGCAAGGCAT 
AAGGTGCCAC CCACACTGCA GCCCCCAGAG AGGGAGGCTG CAGAGAAGGA GAGCAACGCG 
GTGGAAGGGG ATAAGGAAAC CCGAAATCAC CAGCCCAAGG AACCTCACTG TGACCTGGAG 
GCCATTGCAG TTACAGGTGT GGGCCCTCTG CACATGCTGC CCAGCACCTG TCTCCAGAAA 
GTGGACGAGC AACCAGAGGA TGCAGACAAC CAGCGTAATG TCACCCGGAT GGGCAGTCAG 
CCCTCAGATC CCAGCACCAC TGTGCATGTC CCAGTGACAC TGACAGGCCC TCCTGGGGAG 
ACCCCTGTAG TTCCCAGTGG TAACATGAAC CTGGAAGGCC AAGCAGAGGG CAAGAAGGAG 
GCAGAGGCGG ATGATGTGCT GAGAAGAGGC CCCAGGCCCA TCGTTCCCTA CAGCTCCATG 
TTTTGTCTCA GCCCCACCAA CCTGCTTCGT CGCTTCTGCC ATTACATTGT GACCATGCGG 
TACTTTGAGA TGGTAATTCT TGTGGTCATT GCCTTGAGCA GCATTGCCCT GGCTGCAGAG 
GATCCTGTGC GGACAGATTC ATTCAGGAAC AACGCTTTAA AGTACATGGA TTACATCTTT 
ACAGGAGTCT TCACCTTTGA AATGGTCATA AAGATGATAG ACTTGGGCTT GCTGCTGCAC 
CCTGGTGCCT ACTTCCGGGA CCTGTGGAAC ATTCTGGACT TCATTGTTGT CAGTGGAGCC 
CTGGTGGCAT TTGCGTTCTC GAGCTTCATG GGAGGATCCA AAGGGAAAGA CATCAATACC 
ATCAAGTCTC TGAGAGTCCT GCGTGTCCTG AGGCCCCTCA AGACCATCAA GCGGCTGCCT 
AAGCTCAAGG CTGTCTTTGA CTGTGTGGTG AACTCCCTGA AGAACGTCTT GAACATCCTG 
ATTGTCTACA TGCTCTTCAT GTTCATATTT GCCGTCATTG CCGTCCAGCT CTTCAAAGGG 
AAGTTCTTTT ACTGTACTGA TGAATCCAAG GAGCTGGAGA GGGACTGCCG GGGTCAGTAT 
TTGGATTATG AGAAGGAAGA AGTAGAAGCC CAGCCAAGGC AGTGGAAGAA ATATGACTTC 
CACTATGACA ATGTTCTCTG GGCCTTGTTG ACGCTGTTCA CAGTGTCCAC GGGAGAGGGG 
TGGCCCATGG TGCTGAAACA CTCTGTGGAT GCCACCTATG AGGAACAGGG GCCCAGTCCC 
GGGTTCCGGA TGGAGCTCTC CATCTTCTAC GTGGTCTACT TTGTGGTCTT CCCTTTTTTC 
TTTGTCAACA TCTTTGTGGC CTTGATCATT ATCACCTTCC AGGAACAGGG AGATAAGGTG 
ATGTCTGAAT GCAGCTTAGA AAAGAATGAG AGGGCTTGCA TTGATTTTGC CATCAGTGCC 
AAGCCCCTGA CACGGTACAT GCCTCAAAAC AAACAGTCGT TCCAGTATAA GACATGGACA 
TTCGTGGTCT CTCCACCCTT TGAGTACTTC ATCATGGCTA TGATAGCCCT CAACACAGTG 
GTGCTGATGA TGAAGTTCTA TGATGCACCT TATGAGTACG AGCTGATGCT GAAATGCCTG 
AACATTGTCT TCACATCCAT GTTCTCGATG GAGTGCATAC TGAAGATCAT CGCCTTTGGG 
GTATTGAACT ACTTCAGAGA TGCCTGGAAC GTCTTTGACT TTGTCACGGT TTTGGGAAGT 
ATTACTGATA TTTTAGTAAC AGAGATAGCG GAAACGAACA ACTTCATCAA CCTAAGCTTC 
CTTCGCCTCT TCCGGGCGGC ACGGCTGATC AAGCTGCTTC GCCAGGGCTA CACCATCCGC 
ATCCTATTGT GGACCTTCGT CCAGTCCTTT AAGGCGCTGC CCTACGTGTG CCTCCTCATT 
GCCATGCTGT TCTTCATCTA CGCCATCATC GGCATGCAGG TTTTTGGAAA CATTGCCCTT 
GATGATGACA CCAGTATCAA CCGACACAAC AACTTCCGGA CATTTCTGCA AGCCTTAATG 
CTATTGTTCA GGAGTGCCAC TGGGGAGGCC TGGCATGAGA TCATGCTGTC TTGTCTGGGC 
AACCGGGCCT GTGACCCACA TGCCAACGCC AGTGAGTGCG GGAGCGACTT TGCCTATTTT 
TATTTTGTCT CCTTCATCTT CCTCTGTTCC TTTCTGATGT TGAACCTCTT TGTTGCTGTA 
ATCATGGACA ATTTTGAGTA CCTCACTCGG GACTCTTCCA TCCTAGGGCC TCACCACTTA 
GACGAATTCA TTCGAGTCTG GGCTGAATAC GACCCAGCTG CGTGTGGGCG CATCAGTTAC 
AATGACATGT TTGAGATGCT GAAACACATG TCCCCACCTC TGGGGTTGGG GAAGAAATGC 
CCGGCTCGAG TTGCATACAA GCGCCTGGTT CGCATGAACA TGCCCATATC CAATGAGGAC 
ATGACGGTGC ACTTTACGTC CACACTGATG GCCCTCATCC GGACAGCACT GGAGATCAAG 
CTTGCCCCAG CGGGGACGAA GCAGCACCAG TGTGATGCTG AGCTGAGAAA AGAGATCTCT 
TCTGTGTGGG CTAATCTGCC CCAGAAGACT CTGGACTTAC TGGTACCACC CCACAAACCT 
GACGAGATGA CAGTGGGGAA GGTCTATGCT GCTCTCATGA TATTTGACTT CTACAAACAG 
AACAAAACCA CCAGAGATCA GACTCACCAA GCTCCCGGAG GCCTGTCCCA GATGGGTCCC 
GTTTCCCTGT TCCACCCTCT GAAGGCCACC CTGGAACAGA CACAGCCCGC TGTGCTTCGA 
GGAGCTCGGG TTTTCCTTCG GCAAAAGAGT GCAACTTCCC TCAGCAATGG GGGTGCCATA 
CAAACCCAGG AAAGTGGCAT CAAGGAGTCG CTGTCCTGGG GCACGCAGAG GACCCAAGAT 
GCACTTTATG AGGCCAGAGC ACCTCTAGAA CGTGGCCATT CTGCAGAGAT CCCTGTGGGG 
CAGTCAGGAA CACTGGCTGT GGATGTCCAG ATGCAGAACA TGACACTGAG AGGACCAGAT 
GGGGAGCCCC AGCCTGGGCT GGAAAGCCAA GGCAGAGCTG CCTCTATGCC ACGCCTAGCG 
GCAGAAACAC AGCCGGCCCC TAATGCCAGC CCCATGAAGC GCTCCATCTC CACACTGGCT 
CCACGCCCAC ATGGGACTCA GCTTTGCAGC ACAGTTCTGG ACCGGCCTCC TCCTAGCCAG 
GCATCACATC ACCACCACCA CCGCTGCCAC CGGCGCAGAG ACAAGAAGCA AAGGTCCCTG 
GAAAAGGGGC CCAGCCTGTC TGTTGACCCA GAAGGTGCAC CAAGCACTGC TGCAGGACCT 
GGTCTGCCCC ATGGAGAAGG ATCCACCGCC TGCCGGCGGG ACCGTAAACA GGAGCGAGGC 
CGGTCCCAGG AGCGGAGGCA GCCCTCATCT TCCTCTTCAG AGAAGCAGCG CTTCTATTCC 
TGTGACCGCT TTGGGAGCCG GGAGCCCCCG CAACTGATGC CCTCACTCAG TAGCCACCCC 
ACATCGCCAA CAGCGGCGCT AGAGCCAGCA CCCCACCCAC AGGGCAGTGG TTCCGTTAAT 
GGGAGCCCCT TGATGTCAAC ATCCGGTGCT AGCACCCCGG GCCGAGGTGG GCGGAGGCAG 
CTCCCCCAGA CTCCTCTGAC CCCACGCCCC AGCATCACCT ACAAGACCGC CAATTCCTCG 
CCTGTCCACT TTGCTGAGGG TCAGAGCGGC CTCCCAGCCT TCTCCCCTGG CCGTCTCAGC 
CGCGGCCTTT CTGAACACAA TGCCCTGCTC CAGAAAGAGC CCCTGAGCCA GCCTCTAGCT 
CCTGGCTCCC GAATTGGCTC TGACCCTTAC CTAGGGCAGC GTCTGGACAG TGAGGCCTCC 
GCCCACACCC TGCCTGAGGA TACACTCACC TTTGAAGAGG CAGTGGCCAC CAACTCTGGC 
CGCTCCTCCA GGACTTCCTA TGTGTCCTCC CTCACTTCCC AATCCCACCC TCTCCGCCGT 
GTACCCAATG GCTATCACTG CACTTTGGGA CTCAGCACTG GCGTCCGGGC GCGGCACAGC 
TACCACCACC CCGATCAGGA CCACTGGTGC TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cacna1b ( XM_017315141.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017170630.1 Mus musculus calcium channel, voltage-dependent, N type, alpha 1B subunit (Cacna1b), transcript variant X4, mRNA.

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CloneID	OMu80052
Clone ID Related Accession (Same CDS sequence)	XM_017315141.1
Accession Version	XM_017315141.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7050bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	voltage-dependent N-type calcium channel subunit alpha-1B isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTCCGCT TCGGGGACGA GCTAGGCGGC CGCTATGGGG GCACCGGCGG CGGGGAGCGG 
GCTCGGGGTG GCGGGGCCGG CGGGGCGGGT GGCCCGGGCC AGGGGGGTCT GCCGCCGGGC 
CAGCGGGTCC TGTACAAGCA GTCCATTGCG CAGCGCGCAC GGACTATGGC CCTGTACAAC 
CCCATCCCAG TCAAGCAGAA CTGCTTCACC GTCAACCGCT CGCTCTTCGT CTTCAGCGAG 
GACAACGTCG TCCGCAAATA CGCTAAGCGC ATCACCGAAT GGCCGCCCTT CGAATACATG 
ATCCTGGCCA CCATCATCGC CAACTGCATT GTTCTGGCCC TGGAGCAGCA CCTCCCTGAT 
GGGGACAAGA CTCCCATGTC TGAGCGACTA GATGACACGG AGCCTTACTT CATCGGGATC 
TTTTGCTTCG AGGCGGGCAT CAAGATCATA GCCCTGGGCT TTGTTTTCCA CAAGGGCTCC 
TACCTTCGGA ACGGCTGGAA TGTCATGGAC TTCGTGGTGG TCCTCACGGG GATTCTCGCC 
ACAGCTGGAA CTGACTTTGA CCTGCGCACA CTGAGGGCTG TGCGTGTGCT TAGGCCCCTG 
AAGCTGGTGT CTGGAATTCC AAGCTTGCAG GTGGTGCTTA AGTCCATCAT GAAGGCCATG 
GTCCCGCTGC TGCAGATTGG GCTGCTGCTC TTCTTTGCCA TCCTCATGTT TGCCATCATC 
GGCCTCGAAT TCTATATGGG CAAATTCCAT AAGGCCTGTT TCCCCAACAG CACAGATACA 
GAGCCTGTGG GTGACTTTCC CTGTGGCAAA GATCCCCCTG CTCGTCAGTG TGATGGTGAC 
ACCGAATGCC GGGAGTACTG GCCAGGACCC AACTTTGGCA TCACCAATTT TGACAACATC 
CTGTTTGCCA TCTTGACAGT GTTCCAGTGT ATCACCATGG AGGGCTGGAC TGACATCCTC 
TACAATACAA ATGATGCGGC TGGCAACACA TGGAACTGGT TGTACTTCAT CCCCCTCATC 
ATCATTGGCT CCTTCTTCAT GCTCAACCTG GTGCTGGGTG TGCTTTCCGG AGAGTTTGCC 
AAGGAGCGGG AGCGAGTCGA GAACCGCCGC GCCTTCCTGA AGCTCCGCAG GCAGCAGCAG 
ATTGAGCGAG AGCTGAATGG GTACTTGGAG TGGATCTTCA AGGCAGAGGA AGTCATGTTG 
GCAGAGGAGG ACAAGAATGC AGAAGAGAAA TCCCCTTTGG ATGCAGTGTT GAAGAGAGCT 
GCCACCAAGA AGAGCCGAAA TGACCTCATC CATGCAGAAG AGGGGGAGGA CCGGTTTGTA 
GACCTCTGTG CAGTTGGGTC TCCATTTGCT CGTGCCAGCC TCAAGAGTGG GAAGACGGAG 
AGCTCATCGT ACTTCCGGAG AAAGGAGAAG ATGTTCCGGT TCTTTATCCG GCGTATGGTG 
AAAGCACAGA GCTTCTACTG GGTGGTACTG TGTGTGGTGG CCCTGAACAC ACTGTGTGTG 
GCCATGGTGC ACTATAATCA GCCTCAGCGG CTTACCACTG CACTGTACTT TGCAGAGTTT 
GTTTTCCTGG GTCTCTTCCT CACAGAGATG TCCCTGAAGA TGTATGGCCT AGGGCCCAGA 
AGTTACTTCA GGTCTTCCTT CAACTGCTTT GACTTTGGGG TGATTGTGGG GAGTATCTTT 
GAAGTAGTCT GGGCTGCCAT CAAGCCAGGA ACCTCCTTTG GAATCAGTGT GCTGCGGGCT 
CTGCGACTGC TGAGGATATT CAAAGTTACC AAGTATTGGA ACTCTCTGAG GAACCTGGTG 
GTTTCCCTCC TCAATTCCAT GAAGTCCATC ATCAGCCTTC TCTTCCTGCT TTTCCTCTTC 
ATCGTGGTCT TCGCTCTGTT GGGGATGCAG CTGTTTGGGG GACAGTTCAA CTTTCAAGAT 
GAGACTCCAA CCACCAATTT TGATACCTTC CCAGCTGCCA TCCTCACTGT CTTTCAGATC 
CTGACAGGAG AGGATTGGAA TGCCGTAATG TATCATGGGA TTGAGTCGCA AGGTGGAGTC 
AGCAAAGGCA TGTTTTCTTC CTTTTACTTC ATCGTCCTGA CACTGTTTGG AAACTACACC 
CTGCTGAATG TTTTTCTGGC CATTGCTGTG GACAACCTTG CCAATGCCCA GGAGTTGACC 
AAGGATGAAG AGGAGATGGA AGAAGCAGCC AATCAGAAAC TTGCTCTTCA GAAGGCCAAA 
GAAGTAGCTG AAGTCAGCCC CATGTCTGCT GCCAATATCT CCATCGCTGC TTTTGTAAAG 
CAAACTCGAG GTACTGTATC TCGCAGCTCA TCTGTCTCCA GCGTAAACTC ACCCAGGCAG 
CAGAACTCGG CCAAGGCGCG CTCAGTATGG GAGCAGCGGG CCAGTCAGCT AAGGCTCCAG 
AATCTGCGTG CCAGCTGTGA GGCATTGTAC AGTGAGATGG ACCCTGAGGA GCGCCTGCGT 
TATGCCAGCA CGCGCCATGT GAGGCCAGAC ATGAAGACAC ACATGGACCG ACCCCTAGTG 
GTGGAGCCTG GTCGAGATGG CTTGCGGGGA CCCGTTGGGA GCAAGTCAAA GCCTGAAGGC 
ACGGAGGCCA CAGAAAGCGC GGACCTACCT CGCAGGCACC ACCGGCACCG TGATAGGGAC 
AAGACCTCAG CCACAGCACC TGCTGGAGGC GAACAGGACA GGACAGAAAG CACCGAGACC 
GGGGCCCGGG AGGAACGTGC GCGCCCTCGT CGAAGTCACA GCAAGGAGAC TCCAGGGGCT 
GACACGCAAG TGCGCTGTGA GCGCAGTAGA CGTCACCACC GGCGCGGCTC CCCGGAGGAG 
GCCACTGAAC GGGAGCCTCG GCGCCACCGT GCCCACCGGC ATGCACAGGA CTCAAGCAAG 
GAGGGCACGG CGCCGGTGCT TGTACCCAAG GGTGAGCGAC GAGCAAGACA CCGAGGCCCA 
CGCACGGGTC CACGTGAGGC AGAGAACAAC GAGGAGCCCA CACGCAGGCA CCGTGCAAGG 
CATAAGGTGC CACCCACACT GCAGCCCCCA GAGAGGGAGG CTGCAGAGAA GGAGAGCAAC 
GCGGTGGAAG GGGATAAGGA AACCCGAAAT CACCAGCCCA AGGAACCTCA CTGTGACCTG 
GAGGCCATTG CAGTTACAGG TGTGGGCCCT CTGCACATGC TGCCCAGCAC CTGTCTCCAG 
AAAGTGGACG AGCAACCAGA GGATGCAGAC AACCAGCGTA ATGTCACCCG GATGGGCAGT 
CAGCCCTCAG ATCCCAGCAC CACTGTGCAT GTCCCAGTGA CACTGACAGG CCCTCCTGGG 
GAGACCCCTG TAGTTCCCAG TGGTAACATG AACCTGGAAG GCCAAGCAGA GGGCAAGAAG 
GAGGCAGAGG CGGATGATGT GCTGAGAAGA GGCCCCAGGC CCATCGTTCC CTACAGCTCC 
ATGTTTTGTC TCAGCCCCAC CAACCTGCTT CGTCGCTTCT GCCATTACAT TGTGACCATG 
CGGTACTTTG AGATGGTAAT TCTTGTGGTC ATTGCCTTGA GCAGCATTGC CCTGGCTGCA 
GAGGATCCTG TGCGGACAGA TTCATTCAGG AACAACGCTT TAAAGTACAT GGATTACATC 
TTTACAGGAG TCTTCACCTT TGAAATGGTC ATAAAGATGA TAGACTTGGG CTTGCTGCTG 
CACCCTGGTG CCTACTTCCG GGACCTGTGG AACATTCTGG ACTTCATTGT TGTCAGTGGA 
GCCCTGGTGG CATTTGCGTT CTCGAGCTTC ATGGGAGGAT CCAAAGGGAA AGACATCAAT 
ACCATCAAGT CTCTGAGAGT CCTGCGTGTC CTGAGGCCCC TCAAGACCAT CAAGCGGCTG 
CCTAAGCTCA AGGCTGTCTT TGACTGTGTG GTGAACTCCC TGAAGAACGT CTTGAACATC 
CTGATTGTCT ACATGCTCTT CATGTTCATA TTTGCCGTCA TTGCCGTCCA GCTCTTCAAA 
GGGAAGTTCT TTTACTGTAC TGATGAATCC AAGGAGCTGG AGAGGGACTG CCGGGGTCAG 
TATTTGGATT ATGAGAAGGA AGAAGTAGAA GCCCAGCCAA GGCAGTGGAA GAAATATGAC 
TTCCACTATG ACAATGTTCT CTGGGCCTTG TTGACGCTGT TCACAGTGTC CACGGGAGAG 
GGGTGGCCCA TGGTGCTGAA ACACTCTGTG GATGCCACCT ATGAGGAACA GGGGCCCAGT 
CCCGGGTTCC GGATGGAGCT CTCCATCTTC TACGTGGTCT ACTTTGTGGT CTTCCCTTTT 
TTCTTTGTCA ACATCTTTGT GGCCTTGATC ATTATCACCT TCCAGGAACA GGGAGATAAG 
GTGATGTCTG AATGCAGCTT AGAAAAGAAT GAGAGGGCTT GCATTGATTT TGCCATCAGT 
GCCAAGCCCC TGACACGGTA CATGCCTCAA AACAAACAGT CGTTCCAGTA TAAGACATGG 
ACATTCGTGG TCTCTCCACC CTTTGAGTAC TTCATCATGG CTATGATAGC CCTCAACACA 
GTGGTGCTGA TGATGAAGTT CTATGATGCA CCTTATGAGT ACGAGCTGAT GCTGAAATGC 
CTGAACATTG TCTTCACATC CATGTTCTCG ATGGAGTGCA TACTGAAGAT CATCGCCTTT 
GGGGTATTGA ACTACTTCAG AGATGCCTGG AACGTCTTTG ACTTTGTCAC GGTTTTGGGA 
AGTATTACTG ATATTTTAGT AACAGAGATA GCGAACAACT TCATCAACCT AAGCTTCCTT 
CGCCTCTTCC GGGCGGCACG GCTGATCAAG CTGCTTCGCC AGGGCTACAC CATCCGCATC 
CTATTGTGGA CCTTCGTCCA GTCCTTTAAG GCGCTGCCCT ACGTGTGCCT CCTCATTGCC 
ATGCTGTTCT TCATCTACGC CATCATCGGC ATGCAGGTTT TTGGAAACAT TGCCCTTGAT 
GATGACACCA GTATCAACCG ACACAACAAC TTCCGGACAT TTCTGCAAGC CTTAATGCTA 
TTGTTCAGGA GTGCCACTGG GGAGGCCTGG CATGAGATCA TGCTGTCTTG TCTGGGCAAC 
CGGGCCTGTG ACCCACATGC CAACGCCAGT GAGTGCGGGA GCGACTTTGC CTATTTTTAT 
TTTGTCTCCT TCATCTTCCT CTGTTCCTTT CTGATGTTGA ACCTCTTTGT TGCTGTAATC 
ATGGACAATT TTGAGTACCT CACTCGGGAC TCTTCCATCC TAGGGCCTCA CCACTTAGAC 
GAATTCATTC GAGTCTGGGC TGAATACGAC CCAGCTGCGT GTGGGCGCAT CAGTTACAAT 
GACATGTTTG AGATGCTGAA ACACATGTCC CCACCTCTGG GGTTGGGGAA GAAATGCCCG 
GCTCGAGTTG CATACAAGCG CCTGGTTCGC ATGAACATGC CCATATCCAA TGAGGACATG 
ACGGTGCACT TTACGTCCAC ACTGATGGCC CTCATCCGGA CAGCACTGGA GATCAAGCTT 
GCCCCAGCGG GGACGAAGCA GCACCAGTGT GATGCTGAGC TGAGAAAAGA GATCTCTTCT 
GTGTGGGCTA ATCTGCCCCA GAAGACTCTG GACTTACTGG TACCACCCCA CAAACCTGAC 
GAGATGACAG TGGGGAAGGT CTATGCTGCT CTCATGATAT TTGACTTCTA CAAACAGAAC 
AAAACCACCA GAGATCAGAC TCACCAAGCT CCCGGAGGCC TGTCCCAGAT GGGTCCCGTT 
TCCCTGTTCC ACCCTCTGAA GGCCACCCTG GAACAGACAC AGCCCGCTGT GCTTCGAGGA 
GCTCGGGTTT TCCTTCGGCA AAAGAGTGCA ACTTCCCTCA GCAATGGGGG TGCCATACAA 
ACCCAGGAAA GTGGCATCAA GGAGTCGCTG TCCTGGGGCA CGCAGAGGAC CCAAGATGCA 
CTTTATGAGG CCAGAGCACC TCTAGAACGT GGCCATTCTG CAGAGATCCC TGTGGGGCAG 
TCAGGAACAC TGGCTGTGGA TGTCCAGATG CAGAACATGA CACTGAGAGG ACCAGATGGG 
GAGCCCCAGC CTGGGCTGGA AAGCCAAGGC AGAGCTGCCT CTATGCCACG CCTAGCGGCA 
GAAACACAGC CGGCCCCTAA TGCCAGCCCC ATGAAGCGCT CCATCTCCAC ACTGGCTCCA 
CGCCCACATG GGACTCAGCT TTGCAGCACA GTTCTGGACC GGCCTCCTCC TAGCCAGGCA 
TCACATCACC ACCACCACCG CTGCCACCGG CGCAGAGACA AGAAGCAAAG GTCCCTGGAA 
AAGGGGCCCA GCCTGTCTGT TGACCCAGAA GGTGCACCAA GCACTGCTGC AGGACCTGGT 
CTGCCCCATG GAGAAGGATC CACCGCCTGC CGGCGGGACC GTAAACAGGA GCGAGGCCGG 
TCCCAGGAGC GGAGGCAGCC CTCATCTTCC TCTTCAGAGA AGCAGCGCTT CTATTCCTGT 
GACCGCTTTG GGAGCCGGGA GCCCCCGCAA CTGATGCCCT CACTCAGTAG CCACCCCACA 
TCGCCAACAG CGGCGCTAGA GCCAGCACCC CACCCACAGG GCAGTGGTTC CGTTAATGGG 
AGCCCCTTGA TGTCAACATC CGGTGCTAGC ACCCCGGGCC GAGGTGGGCG GAGGCAGCTC 
CCCCAGACTC CTCTGACCCC ACGCCCCAGC ATCACCTACA AGACCGCCAA TTCCTCGCCT 
GTCCACTTTG CTGAGGGTCA GAGCGGCCTC CCAGCCTTCT CCCCTGGCCG TCTCAGCCGC 
GGCCTTTCTG AACACAATGC CCTGCTCCAG AAAGAGCCCC TGAGCCAGCC TCTAGCTCCT 
GGCTCCCGAA TTGGCTCTGA CCCTTACCTA GGGCAGCGTC TGGACAGTGA GGCCTCCGCC 
CACACCCTGC CTGAGGATAC ACTCACCTTT GAAGAGGCAG TGGCCACCAA CTCTGGCCGC 
TCCTCCAGGA CTTCCTATGT GTCCTCCCTC ACTTCCCAAT CCCACCCTCT CCGCCGTGTA 
CCCAATGGCT ATCACTGCAC TTTGGGACTC AGCACTGGCG TCCGGGCGCG GCACAGCTAC 
CACCACCCCG ATCAGGACCA CTGGTGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017170630.1
CDS	146..7195
Translation	MVRFGDELGGRYGGTGGGERARGGGAGGAGGPGQGGLPPGQRVLYKQSIAQRARTMALYNPIPVKQNCFTVNRSLFVFSEDNVVRKYAKRITEWPPFEYMILATIIANCIVLALEQHLPDGDKTPMSERLDDTEPYFIGIFCFEAGIKIIALGFVFHKGSYLRNGWNVMDFVVVLTGILATAGTDFDLRTLRAVRVLRPLKLVSGIPSLQVVLKSIMKAMVPLLQIGLLLFFAILMFAIIGLEFYMGKFHKACFPNSTDTEPVGDFPCGKDPPARQCDGDTECREYWPGPNFGITNFDNILFAILTVFQCITMEGWTDILYNTNDAAGNTWNWLYFIPLIIIGSFFMLNLVLGVLSGEFAKERERVENRRAFLKLRRQQQIERELNGYLEWIFKAEEVMLAEEDKNAEEKSPLDAVLKRAATKKSRNDLIHAEEGEDRFVDLCAVGSPFARASLKSGKTESSSYFRRKEKMFRFFIRRMVKAQSFYWVVLCVVALNTLCVAMVHYNQPQRLTTALYFAEFVFLGLFLTEMSLKMYGLGPRSYFRSSFNCFDFGVIVGSIFEVVWAAIKPGTSFGISVLRALRLLRIFKVTKYWNSLRNLVVSLLNSMKSIISLLFLLFLFIVVFALLGMQLFGGQFNFQDETPTTNFDTFPAAILTVFQILTGEDWNAVMYHGIESQGGVSKGMFSSFYFIVLTLFGNYTLLNVFLAIAVDNLANAQELTKDEEEMEEAANQKLALQKAKEVAEVSPMSAANISIAAFVKQTRGTVSRSSSVSSVNSPRQQNSAKARSVWEQRASQLRLQNLRASCEALYSEMDPEERLRYASTRHVRPDMKTHMDRPLVVEPGRDGLRGPVGSKSKPEGTEATESADLPRRHHRHRDRDKTSATAPAGGEQDRTESTETGAREERARPRRSHSKETPGADTQVRCERSRRHHRRGSPEEATEREPRRHRAHRHAQDSSKEGTAPVLVPKGERRARHRGPRTGPREAENNEEPTRRHRARHKVPPTLQPPEREAAEKESNAVEGDKETRNHQPKEPHCDLEAIAVTGVGPLHMLPSTCLQKVDEQPEDADNQRNVTRMGSQPSDPSTTVHVPVTLTGPPGETPVVPSGNMNLEGQAEGKKEAEADDVLRRGPRPIVPYSSMFCLSPTNLLRRFCHYIVTMRYFEMVILVVIALSSIALAAEDPVRTDSFRNNALKYMDYIFTGVFTFEMVIKMIDLGLLLHPGAYFRDLWNILDFIVVSGALVAFAFSSFMGGSKGKDINTIKSLRVLRVLRPLKTIKRLPKLKAVFDCVVNSLKNVLNILIVYMLFMFIFAVIAVQLFKGKFFYCTDESKELERDCRGQYLDYEKEEVEAQPRQWKKYDFHYDNVLWALLTLFTVSTGEGWPMVLKHSVDATYEEQGPSPGFRMELSIFYVVYFVVFPFFFVNIFVALIIITFQEQGDKVMSECSLEKNERACIDFAISAKPLTRYMPQNKQSFQYKTWTFVVSPPFEYFIMAMIALNTVVLMMKFYDAPYEYELMLKCLNIVFTSMFSMECILKIIAFGVLNYFRDAWNVFDFVTVLGSITDILVTEIANNFINLSFLRLFRAARLIKLLRQGYTIRILLWTFVQSFKALPYVCLLIAMLFFIYAIIGMQVFGNIALDDDTSINRHNNFRTFLQALMLLFRSATGEAWHEIMLSCLGNRACDPHANASECGSDFAYFYFVSFIFLCSFLMLNLFVAVIMDNFEYLTRDSSILGPHHLDEFIRVWAEYDPAACGRISYNDMFEMLKHMSPPLGLGKKCPARVAYKRLVRMNMPISNEDMTVHFTSTLMALIRTALEIKLAPAGTKQHQCDAELRKEISSVWANLPQKTLDLLVPPHKPDEMTVGKVYAALMIFDFYKQNKTTRDQTHQAPGGLSQMGPVSLFHPLKATLEQTQPAVLRGARVFLRQKSATSLSNGGAIQTQESGIKESLSWGTQRTQDALYEARAPLERGHSAEIPVGQSGTLAVDVQMQNMTLRGPDGEPQPGLESQGRAASMPRLAAETQPAPNASPMKRSISTLAPRPHGTQLCSTVLDRPPPSQASHHHHHRCHRRRDKKQRSLEKGPSLSVDPEGAPSTAAGPGLPHGEGSTACRRDRKQERGRSQERRQPSSSSSEKQRFYSCDRFGSREPPQLMPSLSSHPTSPTAALEPAPHPQGSGSVNGSPLMSTSGASTPGRGGRRQLPQTPLTPRPSITYKTANSSPVHFAEGQSGLPAFSPGRLSRGLSEHNALLQKEPLSQPLAPGSRIGSDPYLGQRLDSEASAHTLPEDTLTFEEAVATNSGRSSRTSYVSSLTSQSHPLRRVPNGYHCTLGLSTGVRARHSYHHPDQDHWC

Target ORF information:

RefSeq Version	XM_017315141.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus calcium channel, voltage-dependent, N type, alpha 1B subunit (Cacna1b), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017315141.1

ORF Insert Sequence:

ATGGTCCGCT TCGGGGACGA GCTAGGCGGC CGCTATGGGG GCACCGGCGG CGGGGAGCGG 
GCTCGGGGTG GCGGGGCCGG CGGGGCGGGT GGCCCGGGCC AGGGGGGTCT GCCGCCGGGC 
CAGCGGGTCC TGTACAAGCA GTCCATTGCG CAGCGCGCAC GGACTATGGC CCTGTACAAC 
CCCATCCCAG TCAAGCAGAA CTGCTTCACC GTCAACCGCT CGCTCTTCGT CTTCAGCGAG 
GACAACGTCG TCCGCAAATA CGCTAAGCGC ATCACCGAAT GGCCGCCCTT CGAATACATG 
ATCCTGGCCA CCATCATCGC CAACTGCATT GTTCTGGCCC TGGAGCAGCA CCTCCCTGAT 
GGGGACAAGA CTCCCATGTC TGAGCGACTA GATGACACGG AGCCTTACTT CATCGGGATC 
TTTTGCTTCG AGGCGGGCAT CAAGATCATA GCCCTGGGCT TTGTTTTCCA CAAGGGCTCC 
TACCTTCGGA ACGGCTGGAA TGTCATGGAC TTCGTGGTGG TCCTCACGGG GATTCTCGCC 
ACAGCTGGAA CTGACTTTGA CCTGCGCACA CTGAGGGCTG TGCGTGTGCT TAGGCCCCTG 
AAGCTGGTGT CTGGAATTCC AAGCTTGCAG GTGGTGCTTA AGTCCATCAT GAAGGCCATG 
GTCCCGCTGC TGCAGATTGG GCTGCTGCTC TTCTTTGCCA TCCTCATGTT TGCCATCATC 
GGCCTCGAAT TCTATATGGG CAAATTCCAT AAGGCCTGTT TCCCCAACAG CACAGATACA 
GAGCCTGTGG GTGACTTTCC CTGTGGCAAA GATCCCCCTG CTCGTCAGTG TGATGGTGAC 
ACCGAATGCC GGGAGTACTG GCCAGGACCC AACTTTGGCA TCACCAATTT TGACAACATC 
CTGTTTGCCA TCTTGACAGT GTTCCAGTGT ATCACCATGG AGGGCTGGAC TGACATCCTC 
TACAATACAA ATGATGCGGC TGGCAACACA TGGAACTGGT TGTACTTCAT CCCCCTCATC 
ATCATTGGCT CCTTCTTCAT GCTCAACCTG GTGCTGGGTG TGCTTTCCGG AGAGTTTGCC 
AAGGAGCGGG AGCGAGTCGA GAACCGCCGC GCCTTCCTGA AGCTCCGCAG GCAGCAGCAG 
ATTGAGCGAG AGCTGAATGG GTACTTGGAG TGGATCTTCA AGGCAGAGGA AGTCATGTTG 
GCAGAGGAGG ACAAGAATGC AGAAGAGAAA TCCCCTTTGG ATGCAGTGTT GAAGAGAGCT 
GCCACCAAGA AGAGCCGAAA TGACCTCATC CATGCAGAAG AGGGGGAGGA CCGGTTTGTA 
GACCTCTGTG CAGTTGGGTC TCCATTTGCT CGTGCCAGCC TCAAGAGTGG GAAGACGGAG 
AGCTCATCGT ACTTCCGGAG AAAGGAGAAG ATGTTCCGGT TCTTTATCCG GCGTATGGTG 
AAAGCACAGA GCTTCTACTG GGTGGTACTG TGTGTGGTGG CCCTGAACAC ACTGTGTGTG 
GCCATGGTGC ACTATAATCA GCCTCAGCGG CTTACCACTG CACTGTACTT TGCAGAGTTT 
GTTTTCCTGG GTCTCTTCCT CACAGAGATG TCCCTGAAGA TGTATGGCCT AGGGCCCAGA 
AGTTACTTCA GGTCTTCCTT CAACTGCTTT GACTTTGGGG TGATTGTGGG GAGTATCTTT 
GAAGTAGTCT GGGCTGCCAT CAAGCCAGGA ACCTCCTTTG GAATCAGTGT GCTGCGGGCT 
CTGCGACTGC TGAGGATATT CAAAGTTACC AAGTATTGGA ACTCTCTGAG GAACCTGGTG 
GTTTCCCTCC TCAATTCCAT GAAGTCCATC ATCAGCCTTC TCTTCCTGCT TTTCCTCTTC 
ATCGTGGTCT TCGCTCTGTT GGGGATGCAG CTGTTTGGGG GACAGTTCAA CTTTCAAGAT 
GAGACTCCAA CCACCAATTT TGATACCTTC CCAGCTGCCA TCCTCACTGT CTTTCAGATC 
CTGACAGGAG AGGATTGGAA TGCCGTAATG TATCATGGGA TTGAGTCGCA AGGTGGAGTC 
AGCAAAGGCA TGTTTTCTTC CTTTTACTTC ATCGTCCTGA CACTGTTTGG AAACTACACC 
CTGCTGAATG TTTTTCTGGC CATTGCTGTG GACAACCTTG CCAATGCCCA GGAGTTGACC 
AAGGATGAAG AGGAGATGGA AGAAGCAGCC AATCAGAAAC TTGCTCTTCA GAAGGCCAAA 
GAAGTAGCTG AAGTCAGCCC CATGTCTGCT GCCAATATCT CCATCGCTGC TTTTGTAAAG 
CAAACTCGAG GTACTGTATC TCGCAGCTCA TCTGTCTCCA GCGTAAACTC ACCCAGGCAG 
CAGAACTCGG CCAAGGCGCG CTCAGTATGG GAGCAGCGGG CCAGTCAGCT AAGGCTCCAG 
AATCTGCGTG CCAGCTGTGA GGCATTGTAC AGTGAGATGG ACCCTGAGGA GCGCCTGCGT 
TATGCCAGCA CGCGCCATGT GAGGCCAGAC ATGAAGACAC ACATGGACCG ACCCCTAGTG 
GTGGAGCCTG GTCGAGATGG CTTGCGGGGA CCCGTTGGGA GCAAGTCAAA GCCTGAAGGC 
ACGGAGGCCA CAGAAAGCGC GGACCTACCT CGCAGGCACC ACCGGCACCG TGATAGGGAC 
AAGACCTCAG CCACAGCACC TGCTGGAGGC GAACAGGACA GGACAGAAAG CACCGAGACC 
GGGGCCCGGG AGGAACGTGC GCGCCCTCGT CGAAGTCACA GCAAGGAGAC TCCAGGGGCT 
GACACGCAAG TGCGCTGTGA GCGCAGTAGA CGTCACCACC GGCGCGGCTC CCCGGAGGAG 
GCCACTGAAC GGGAGCCTCG GCGCCACCGT GCCCACCGGC ATGCACAGGA CTCAAGCAAG 
GAGGGCACGG CGCCGGTGCT TGTACCCAAG GGTGAGCGAC GAGCAAGACA CCGAGGCCCA 
CGCACGGGTC CACGTGAGGC AGAGAACAAC GAGGAGCCCA CACGCAGGCA CCGTGCAAGG 
CATAAGGTGC CACCCACACT GCAGCCCCCA GAGAGGGAGG CTGCAGAGAA GGAGAGCAAC 
GCGGTGGAAG GGGATAAGGA AACCCGAAAT CACCAGCCCA AGGAACCTCA CTGTGACCTG 
GAGGCCATTG CAGTTACAGG TGTGGGCCCT CTGCACATGC TGCCCAGCAC CTGTCTCCAG 
AAAGTGGACG AGCAACCAGA GGATGCAGAC AACCAGCGTA ATGTCACCCG GATGGGCAGT 
CAGCCCTCAG ATCCCAGCAC CACTGTGCAT GTCCCAGTGA CACTGACAGG CCCTCCTGGG 
GAGACCCCTG TAGTTCCCAG TGGTAACATG AACCTGGAAG GCCAAGCAGA GGGCAAGAAG 
GAGGCAGAGG CGGATGATGT GCTGAGAAGA GGCCCCAGGC CCATCGTTCC CTACAGCTCC 
ATGTTTTGTC TCAGCCCCAC CAACCTGCTT CGTCGCTTCT GCCATTACAT TGTGACCATG 
CGGTACTTTG AGATGGTAAT TCTTGTGGTC ATTGCCTTGA GCAGCATTGC CCTGGCTGCA 
GAGGATCCTG TGCGGACAGA TTCATTCAGG AACAACGCTT TAAAGTACAT GGATTACATC 
TTTACAGGAG TCTTCACCTT TGAAATGGTC ATAAAGATGA TAGACTTGGG CTTGCTGCTG 
CACCCTGGTG CCTACTTCCG GGACCTGTGG AACATTCTGG ACTTCATTGT TGTCAGTGGA 
GCCCTGGTGG CATTTGCGTT CTCGAGCTTC ATGGGAGGAT CCAAAGGGAA AGACATCAAT 
ACCATCAAGT CTCTGAGAGT CCTGCGTGTC CTGAGGCCCC TCAAGACCAT CAAGCGGCTG 
CCTAAGCTCA AGGCTGTCTT TGACTGTGTG GTGAACTCCC TGAAGAACGT CTTGAACATC 
CTGATTGTCT ACATGCTCTT CATGTTCATA TTTGCCGTCA TTGCCGTCCA GCTCTTCAAA 
GGGAAGTTCT TTTACTGTAC TGATGAATCC AAGGAGCTGG AGAGGGACTG CCGGGGTCAG 
TATTTGGATT ATGAGAAGGA AGAAGTAGAA GCCCAGCCAA GGCAGTGGAA GAAATATGAC 
TTCCACTATG ACAATGTTCT CTGGGCCTTG TTGACGCTGT TCACAGTGTC CACGGGAGAG 
GGGTGGCCCA TGGTGCTGAA ACACTCTGTG GATGCCACCT ATGAGGAACA GGGGCCCAGT 
CCCGGGTTCC GGATGGAGCT CTCCATCTTC TACGTGGTCT ACTTTGTGGT CTTCCCTTTT 
TTCTTTGTCA ACATCTTTGT GGCCTTGATC ATTATCACCT TCCAGGAACA GGGAGATAAG 
GTGATGTCTG AATGCAGCTT AGAAAAGAAT GAGAGGGCTT GCATTGATTT TGCCATCAGT 
GCCAAGCCCC TGACACGGTA CATGCCTCAA AACAAACAGT CGTTCCAGTA TAAGACATGG 
ACATTCGTGG TCTCTCCACC CTTTGAGTAC TTCATCATGG CTATGATAGC CCTCAACACA 
GTGGTGCTGA TGATGAAGTT CTATGATGCA CCTTATGAGT ACGAGCTGAT GCTGAAATGC 
CTGAACATTG TCTTCACATC CATGTTCTCG ATGGAGTGCA TACTGAAGAT CATCGCCTTT 
GGGGTATTGA ACTACTTCAG AGATGCCTGG AACGTCTTTG ACTTTGTCAC GGTTTTGGGA 
AGTATTACTG ATATTTTAGT AACAGAGATA GCGAACAACT TCATCAACCT AAGCTTCCTT 
CGCCTCTTCC GGGCGGCACG GCTGATCAAG CTGCTTCGCC AGGGCTACAC CATCCGCATC 
CTATTGTGGA CCTTCGTCCA GTCCTTTAAG GCGCTGCCCT ACGTGTGCCT CCTCATTGCC 
ATGCTGTTCT TCATCTACGC CATCATCGGC ATGCAGGTTT TTGGAAACAT TGCCCTTGAT 
GATGACACCA GTATCAACCG ACACAACAAC TTCCGGACAT TTCTGCAAGC CTTAATGCTA 
TTGTTCAGGA GTGCCACTGG GGAGGCCTGG CATGAGATCA TGCTGTCTTG TCTGGGCAAC 
CGGGCCTGTG ACCCACATGC CAACGCCAGT GAGTGCGGGA GCGACTTTGC CTATTTTTAT 
TTTGTCTCCT TCATCTTCCT CTGTTCCTTT CTGATGTTGA ACCTCTTTGT TGCTGTAATC 
ATGGACAATT TTGAGTACCT CACTCGGGAC TCTTCCATCC TAGGGCCTCA CCACTTAGAC 
GAATTCATTC GAGTCTGGGC TGAATACGAC CCAGCTGCGT GTGGGCGCAT CAGTTACAAT 
GACATGTTTG AGATGCTGAA ACACATGTCC CCACCTCTGG GGTTGGGGAA GAAATGCCCG 
GCTCGAGTTG CATACAAGCG CCTGGTTCGC ATGAACATGC CCATATCCAA TGAGGACATG 
ACGGTGCACT TTACGTCCAC ACTGATGGCC CTCATCCGGA CAGCACTGGA GATCAAGCTT 
GCCCCAGCGG GGACGAAGCA GCACCAGTGT GATGCTGAGC TGAGAAAAGA GATCTCTTCT 
GTGTGGGCTA ATCTGCCCCA GAAGACTCTG GACTTACTGG TACCACCCCA CAAACCTGAC 
GAGATGACAG TGGGGAAGGT CTATGCTGCT CTCATGATAT TTGACTTCTA CAAACAGAAC 
AAAACCACCA GAGATCAGAC TCACCAAGCT CCCGGAGGCC TGTCCCAGAT GGGTCCCGTT 
TCCCTGTTCC ACCCTCTGAA GGCCACCCTG GAACAGACAC AGCCCGCTGT GCTTCGAGGA 
GCTCGGGTTT TCCTTCGGCA AAAGAGTGCA ACTTCCCTCA GCAATGGGGG TGCCATACAA 
ACCCAGGAAA GTGGCATCAA GGAGTCGCTG TCCTGGGGCA CGCAGAGGAC CCAAGATGCA 
CTTTATGAGG CCAGAGCACC TCTAGAACGT GGCCATTCTG CAGAGATCCC TGTGGGGCAG 
TCAGGAACAC TGGCTGTGGA TGTCCAGATG CAGAACATGA CACTGAGAGG ACCAGATGGG 
GAGCCCCAGC CTGGGCTGGA AAGCCAAGGC AGAGCTGCCT CTATGCCACG CCTAGCGGCA 
GAAACACAGC CGGCCCCTAA TGCCAGCCCC ATGAAGCGCT CCATCTCCAC ACTGGCTCCA 
CGCCCACATG GGACTCAGCT TTGCAGCACA GTTCTGGACC GGCCTCCTCC TAGCCAGGCA 
TCACATCACC ACCACCACCG CTGCCACCGG CGCAGAGACA AGAAGCAAAG GTCCCTGGAA 
AAGGGGCCCA GCCTGTCTGT TGACCCAGAA GGTGCACCAA GCACTGCTGC AGGACCTGGT 
CTGCCCCATG GAGAAGGATC CACCGCCTGC CGGCGGGACC GTAAACAGGA GCGAGGCCGG 
TCCCAGGAGC GGAGGCAGCC CTCATCTTCC TCTTCAGAGA AGCAGCGCTT CTATTCCTGT 
GACCGCTTTG GGAGCCGGGA GCCCCCGCAA CTGATGCCCT CACTCAGTAG CCACCCCACA 
TCGCCAACAG CGGCGCTAGA GCCAGCACCC CACCCACAGG GCAGTGGTTC CGTTAATGGG 
AGCCCCTTGA TGTCAACATC CGGTGCTAGC ACCCCGGGCC GAGGTGGGCG GAGGCAGCTC 
CCCCAGACTC CTCTGACCCC ACGCCCCAGC ATCACCTACA AGACCGCCAA TTCCTCGCCT 
GTCCACTTTG CTGAGGGTCA GAGCGGCCTC CCAGCCTTCT CCCCTGGCCG TCTCAGCCGC 
GGCCTTTCTG AACACAATGC CCTGCTCCAG AAAGAGCCCC TGAGCCAGCC TCTAGCTCCT 
GGCTCCCGAA TTGGCTCTGA CCCTTACCTA GGGCAGCGTC TGGACAGTGA GGCCTCCGCC 
CACACCCTGC CTGAGGATAC ACTCACCTTT GAAGAGGCAG TGGCCACCAA CTCTGGCCGC 
TCCTCCAGGA CTTCCTATGT GTCCTCCCTC ACTTCCCAAT CCCACCCTCT CCGCCGTGTA 
CCCAATGGCT ATCACTGCAC TTTGGGACTC AGCACTGGCG TCCGGGCGCG GCACAGCTAC 
CACCACCCCG ATCAGGACCA CTGGTGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cacna1b ( XM_017315146.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017170635.1 Mus musculus calcium channel, voltage-dependent, N type, alpha 1B subunit (Cacna1b), transcript variant X9, mRNA.

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CloneID	OMu80053
Clone ID Related Accession (Same CDS sequence)	XM_017315146.1
Accession Version	XM_017315146.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6990bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	voltage-dependent N-type calcium channel subunit alpha-1B isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTCCGCT TCGGGGACGA GCTAGGCGGC CGCTATGGGG GCACCGGCGG CGGGGAGCGG 
GCTCGGGGTG GCGGGGCCGG CGGGGCGGGT GGCCCGGGCC AGGGGGGTCT GCCGCCGGGC 
CAGCGGGTCC TGTACAAGCA GTCCATTGCG CAGCGCGCAC GGACTATGGC CCTGTACAAC 
CCCATCCCAG TCAAGCAGAA CTGCTTCACC GTCAACCGCT CGCTCTTCGT CTTCAGCGAG 
GACAACGTCG TCCGCAAATA CGCTAAGCGC ATCACCGAAT GGCCGCCCTT CGAATACATG 
ATCCTGGCCA CCATCATCGC CAACTGCATT GTTCTGGCCC TGGAGCAGCA CCTCCCTGAT 
GGGGACAAGA CTCCCATGTC TGAGCGACTA GATGACACGG AGCCTTACTT CATCGGGATC 
TTTTGCTTCG AGGCGGGCAT CAAGATCATA GCCCTGGGCT TTGTTTTCCA CAAGGGCTCC 
TACCTTCGGA ACGGCTGGAA TGTCATGGAC TTCGTGGTGG TCCTCACGGG GATTCTCGCC 
ACAGCTGGAA CTGACTTTGA CCTGCGCACA CTGAGGGCTG TGCGTGTGCT TAGGCCCCTG 
AAGCTGGTGT CTGGAATTCC AAGCTTGCAG GTGGTGCTTA AGTCCATCAT GAAGGCCATG 
GTCCCGCTGC TGCAGATTGG GCTGCTGCTC TTCTTTGCCA TCCTCATGTT TGCCATCATC 
GGCCTCGAAT TCTATATGGG CAAATTCCAT AAGGCCTGTT TCCCCAACAG CACAGATACA 
GAGCCTGTGG GTGACTTTCC CTGTGGCAAA GATCCCCCTG CTCGTCAGTG TGATGGTGAC 
ACCGAATGCC GGGAGTACTG GCCAGGACCC AACTTTGGCA TCACCAATTT TGACAACATC 
CTGTTTGCCA TCTTGACAGT GTTCCAGTGT ATCACCATGG AGGGCTGGAC TGACATCCTC 
TACAATACAA ATGATGCGGC TGGCAACACA TGGAACTGGT TGTACTTCAT CCCCCTCATC 
ATCATTGGCT CCTTCTTCAT GCTCAACCTG GTGCTGGGTG TGCTTTCCGG AGAGTTTGCC 
AAGGAGCGGG AGCGAGTCGA GAACCGCCGC GCCTTCCTGA AGCTCCGCAG GCAGCAGCAG 
ATTGAGCGAG AGCTGAATGG GTACTTGGAG TGGATCTTCA AGGCAGAGGA AGTCATGTTG 
GCAGAGGAGG ACAAGAATGC AGAAGAGAAA TCCCCTTTGG ATGCAGTGTT GAAGAGAGCT 
GCCACCAAGA AGAGCCGAAA TGACCTCATC CATGCAGAAG AGGGGGAGGA CCGGTTTGTA 
GACCTCTGTG CAGTTGGGTC TCCATTTGCT CGTGCCAGCC TCAAGAGTGG GAAGACGGAG 
AGCTCATCGT ACTTCCGGAG AAAGGAGAAG ATGTTCCGGT TCTTTATCCG GCGTATGGTG 
AAAGCACAGA GCTTCTACTG GGTGGTACTG TGTGTGGTGG CCCTGAACAC ACTGTGTGTG 
GCCATGGTGC ACTATAATCA GCCTCAGCGG CTTACCACTG CACTGTACTT TGCAGAGTTT 
GTTTTCCTGG GTCTCTTCCT CACAGAGATG TCCCTGAAGA TGTATGGCCT AGGGCCCAGA 
AGTTACTTCA GGTCTTCCTT CAACTGCTTT GACTTTGGGG TGATTGTGGG GAGTATCTTT 
GAAGTAGTCT GGGCTGCCAT CAAGCCAGGA ACCTCCTTTG GAATCAGTGT GCTGCGGGCT 
CTGCGACTGC TGAGGATATT CAAAGTTACC AAGTATTGGA ACTCTCTGAG GAACCTGGTG 
GTTTCCCTCC TCAATTCCAT GAAGTCCATC ATCAGCCTTC TCTTCCTGCT TTTCCTCTTC 
ATCGTGGTCT TCGCTCTGTT GGGGATGCAG CTGTTTGGGG GACAGTTCAA CTTTCAAGAT 
GAGACTCCAA CCACCAATTT TGATACCTTC CCAGCTGCCA TCCTCACTGT CTTTCAGATC 
CTGACAGGAG AGGATTGGAA TGCCGTAATG TATCATGGGA TTGAGTCGCA AGGTGGAGTC 
AGCAAAGGCA TGTTTTCTTC CTTTTACTTC ATCGTCCTGA CACTGTTTGG AAACTACACC 
CTGCTGAATG TTTTTCTGGC CATTGCTGTG GACAACCTTG CCAATGCCCA GGAGTTGACC 
AAGGATGAAG AGGAGATGGA AGAAGCAGCC AATCAGAAAC TTGCTCTTCA GAAGGCCAAA 
GAAGTAGCTG AAGTCAGCCC CATGTCTGCT GCCAATATCT CCATCGCTGC GCAGCAGAAC 
TCGGCCAAGG CGCGCTCAGT ATGGGAGCAG CGGGCCAGTC AGCTAAGGCT CCAGAATCTG 
CGTGCCAGCT GTGAGGCATT GTACAGTGAG ATGGACCCTG AGGAGCGCCT GCGTTATGCC 
AGCACGCGCC ATGTGAGGCC AGACATGAAG ACACACATGG ACCGACCCCT AGTGGTGGAG 
CCTGGTCGAG ATGGCTTGCG GGGACCCGTT GGGAGCAAGT CAAAGCCTGA AGGCACGGAG 
GCCACAGAAA GCGCGGACCT ACCTCGCAGG CACCACCGGC ACCGTGATAG GGACAAGACC 
TCAGCCACAG CACCTGCTGG AGGCGAACAG GACAGGACAG AAAGCACCGA GACCGGGGCC 
CGGGAGGAAC GTGCGCGCCC TCGTCGAAGT CACAGCAAGG AGACTCCAGG GGCTGACACG 
CAAGTGCGCT GTGAGCGCAG TAGACGTCAC CACCGGCGCG GCTCCCCGGA GGAGGCCACT 
GAACGGGAGC CTCGGCGCCA CCGTGCCCAC CGGCATGCAC AGGACTCAAG CAAGGAGGGC 
ACGGCGCCGG TGCTTGTACC CAAGGGTGAG CGACGAGCAA GACACCGAGG CCCACGCACG 
GGTCCACGTG AGGCAGAGAA CAACGAGGAG CCCACACGCA GGCACCGTGC AAGGCATAAG 
GTGCCACCCA CACTGCAGCC CCCAGAGAGG GAGGCTGCAG AGAAGGAGAG CAACGCGGTG 
GAAGGGGATA AGGAAACCCG AAATCACCAG CCCAAGGAAC CTCACTGTGA CCTGGAGGCC 
ATTGCAGTTA CAGGTGTGGG CCCTCTGCAC ATGCTGCCCA GCACCTGTCT CCAGAAAGTG 
GACGAGCAAC CAGAGGATGC AGACAACCAG CGTAATGTCA CCCGGATGGG CAGTCAGCCC 
TCAGATCCCA GCACCACTGT GCATGTCCCA GTGACACTGA CAGGCCCTCC TGGGGAGACC 
CCTGTAGTTC CCAGTGGTAA CATGAACCTG GAAGGCCAAG CAGAGGGCAA GAAGGAGGCA 
GAGGCGGATG ATGTGCTGAG AAGAGGCCCC AGGCCCATCG TTCCCTACAG CTCCATGTTT 
TGTCTCAGCC CCACCAACCT GCTTCGTCGC TTCTGCCATT ACATTGTGAC CATGCGGTAC 
TTTGAGATGG TAATTCTTGT GGTCATTGCC TTGAGCAGCA TTGCCCTGGC TGCAGAGGAT 
CCTGTGCGGA CAGATTCATT CAGGAACAAC GCTTTAAAGT ACATGGATTA CATCTTTACA 
GGAGTCTTCA CCTTTGAAAT GGTCATAAAG ATGATAGACT TGGGCTTGCT GCTGCACCCT 
GGTGCCTACT TCCGGGACCT GTGGAACATT CTGGACTTCA TTGTTGTCAG TGGAGCCCTG 
GTGGCATTTG CGTTCTCGAG CTTCATGGGA GGATCCAAAG GGAAAGACAT CAATACCATC 
AAGTCTCTGA GAGTCCTGCG TGTCCTGAGG CCCCTCAAGA CCATCAAGCG GCTGCCTAAG 
CTCAAGGCTG TCTTTGACTG TGTGGTGAAC TCCCTGAAGA ACGTCTTGAA CATCCTGATT 
GTCTACATGC TCTTCATGTT CATATTTGCC GTCATTGCCG TCCAGCTCTT CAAAGGGAAG 
TTCTTTTACT GTACTGATGA ATCCAAGGAG CTGGAGAGGG ACTGCCGGGG TCAGTATTTG 
GATTATGAGA AGGAAGAAGT AGAAGCCCAG CCAAGGCAGT GGAAGAAATA TGACTTCCAC 
TATGACAATG TTCTCTGGGC CTTGTTGACG CTGTTCACAG TGTCCACGGG AGAGGGGTGG 
CCCATGGTGC TGAAACACTC TGTGGATGCC ACCTATGAGG AACAGGGGCC CAGTCCCGGG 
TTCCGGATGG AGCTCTCCAT CTTCTACGTG GTCTACTTTG TGGTCTTCCC TTTTTTCTTT 
GTCAACATCT TTGTGGCCTT GATCATTATC ACCTTCCAGG AACAGGGAGA TAAGGTGATG 
TCTGAATGCA GCTTAGAAAA GAATGAGAGG GCTTGCATTG ATTTTGCCAT CAGTGCCAAG 
CCCCTGACAC GGTACATGCC TCAAAACAAA CAGTCGTTCC AGTATAAGAC ATGGACATTC 
GTGGTCTCTC CACCCTTTGA GTACTTCATC ATGGCTATGA TAGCCCTCAA CACAGTGGTG 
CTGATGATGA AGTTCTATGA TGCACCTTAT GAGTACGAGC TGATGCTGAA ATGCCTGAAC 
ATTGTCTTCA CATCCATGTT CTCGATGGAG TGCATACTGA AGATCATCGC CTTTGGGGTA 
TTGAACTACT TCAGAGATGC CTGGAACGTC TTTGACTTTG TCACGGTTTT GGGAAGTATT 
ACTGATATTT TAGTAACAGA GATAGCGGAA ACGAACAACT TCATCAACCT AAGCTTCCTT 
CGCCTCTTCC GGGCGGCACG GCTGATCAAG CTGCTTCGCC AGGGCTACAC CATCCGCATC 
CTATTGTGGA CCTTCGTCCA GTCCTTTAAG GCGCTGCCCT ACGTGTGCCT CCTCATTGCC 
ATGCTGTTCT TCATCTACGC CATCATCGGC ATGCAGGTTT TTGGAAACAT TGCCCTTGAT 
GATGACACCA GTATCAACCG ACACAACAAC TTCCGGACAT TTCTGCAAGC CTTAATGCTA 
TTGTTCAGGA GTGCCACTGG GGAGGCCTGG CATGAGATCA TGCTGTCTTG TCTGGGCAAC 
CGGGCCTGTG ACCCACATGC CAACGCCAGT GAGTGCGGGA GCGACTTTGC CTATTTTTAT 
TTTGTCTCCT TCATCTTCCT CTGTTCCTTT CTGATGTTGA ACCTCTTTGT TGCTGTAATC 
ATGGACAATT TTGAGTACCT CACTCGGGAC TCTTCCATCC TAGGGCCTCA CCACTTAGAC 
GAATTCATTC GAGTCTGGGC TGAATACGAC CCAGCTGCGT GTGGGCGCAT CAGTTACAAT 
GACATGTTTG AGATGCTGAA ACACATGTCC CCACCTCTGG GGTTGGGGAA GAAATGCCCG 
GCTCGAGTTG CATACAAGCG CCTGGTTCGC ATGAACATGC CCATATCCAA TGAGGACATG 
ACGGTGCACT TTACGTCCAC ACTGATGGCC CTCATCCGGA CAGCACTGGA GATCAAGCTT 
GCCCCAGCGG GGACGAAGCA GCACCAGTGT GATGCTGAGC TGAGAAAAGA GATCTCTTCT 
GTGTGGGCTA ATCTGCCCCA GAAGACTCTG GACTTACTGG TACCACCCCA CAAACCTGAC 
GAGATGACAG TGGGGAAGGT CTATGCTGCT CTCATGATAT TTGACTTCTA CAAACAGAAC 
AAAACCACCA GAGATCAGAC TCACCAAGCT CCCGGAGGCC TGTCCCAGAT GGGTCCCGTT 
TCCCTGTTCC ACCCTCTGAA GGCCACCCTG GAACAGACAC AGCCCGCTGT GCTTCGAGGA 
GCTCGGGTTT TCCTTCGGCA AAAGAGTGCA ACTTCCCTCA GCAATGGGGG TGCCATACAA 
ACCCAGGAAA GTGGCATCAA GGAGTCGCTG TCCTGGGGCA CGCAGAGGAC CCAAGATGCA 
CTTTATGAGG CCAGAGCACC TCTAGAACGT GGCCATTCTG CAGAGATCCC TGTGGGGCAG 
TCAGGAACAC TGGCTGTGGA TGTCCAGATG CAGAACATGA CACTGAGAGG ACCAGATGGG 
GAGCCCCAGC CTGGGCTGGA AAGCCAAGGC AGAGCTGCCT CTATGCCACG CCTAGCGGCA 
GAAACACAGC CGGCCCCTAA TGCCAGCCCC ATGAAGCGCT CCATCTCCAC ACTGGCTCCA 
CGCCCACATG GGACTCAGCT TTGCAGCACA GTTCTGGACC GGCCTCCTCC TAGCCAGGCA 
TCACATCACC ACCACCACCG CTGCCACCGG CGCAGAGACA AGAAGCAAAG GTCCCTGGAA 
AAGGGGCCCA GCCTGTCTGT TGACCCAGAA GGTGCACCAA GCACTGCTGC AGGACCTGGT 
CTGCCCCATG GAGAAGGATC CACCGCCTGC CGGCGGGACC GTAAACAGGA GCGAGGCCGG 
TCCCAGGAGC GGAGGCAGCC CTCATCTTCC TCTTCAGAGA AGCAGCGCTT CTATTCCTGT 
GACCGCTTTG GGAGCCGGGA GCCCCCGCAA CTGATGCCCT CACTCAGTAG CCACCCCACA 
TCGCCAACAG CGGCGCTAGA GCCAGCACCC CACCCACAGG GCAGTGGTTC CGTTAATGGG 
AGCCCCTTGA TGTCAACATC CGGTGCTAGC ACCCCGGGCC GAGGTGGGCG GAGGCAGCTC 
CCCCAGACTC CTCTGACCCC ACGCCCCAGC ATCACCTACA AGACCGCCAA TTCCTCGCCT 
GTCCACTTTG CTGAGGGTCA GAGCGGCCTC CCAGCCTTCT CCCCTGGCCG TCTCAGCCGC 
GGCCTTTCTG AACACAATGC CCTGCTCCAG AAAGAGCCCC TGAGCCAGCC TCTAGCTCCT 
GGCTCCCGAA TTGGCTCTGA CCCTTACCTA GGGCAGCGTC TGGACAGTGA GGCCTCCGCC 
CACACCCTGC CTGAGGATAC ACTCACCTTT GAAGAGGCAG TGGCCACCAA CTCTGGCCGC 
TCCTCCAGGA CTTCCTATGT GTCCTCCCTC ACTTCCCAAT CCCACCCTCT CCGCCGTGTA 
CCCAATGGCT ATCACTGCAC TTTGGGACTC AGCACTGGCG TCCGGGCGCG GCACAGCTAC 
CACCACCCCG ATCAGGACCA CTGGTGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017170635.1
CDS	146..7135
Translation	MVRFGDELGGRYGGTGGGERARGGGAGGAGGPGQGGLPPGQRVLYKQSIAQRARTMALYNPIPVKQNCFTVNRSLFVFSEDNVVRKYAKRITEWPPFEYMILATIIANCIVLALEQHLPDGDKTPMSERLDDTEPYFIGIFCFEAGIKIIALGFVFHKGSYLRNGWNVMDFVVVLTGILATAGTDFDLRTLRAVRVLRPLKLVSGIPSLQVVLKSIMKAMVPLLQIGLLLFFAILMFAIIGLEFYMGKFHKACFPNSTDTEPVGDFPCGKDPPARQCDGDTECREYWPGPNFGITNFDNILFAILTVFQCITMEGWTDILYNTNDAAGNTWNWLYFIPLIIIGSFFMLNLVLGVLSGEFAKERERVENRRAFLKLRRQQQIERELNGYLEWIFKAEEVMLAEEDKNAEEKSPLDAVLKRAATKKSRNDLIHAEEGEDRFVDLCAVGSPFARASLKSGKTESSSYFRRKEKMFRFFIRRMVKAQSFYWVVLCVVALNTLCVAMVHYNQPQRLTTALYFAEFVFLGLFLTEMSLKMYGLGPRSYFRSSFNCFDFGVIVGSIFEVVWAAIKPGTSFGISVLRALRLLRIFKVTKYWNSLRNLVVSLLNSMKSIISLLFLLFLFIVVFALLGMQLFGGQFNFQDETPTTNFDTFPAAILTVFQILTGEDWNAVMYHGIESQGGVSKGMFSSFYFIVLTLFGNYTLLNVFLAIAVDNLANAQELTKDEEEMEEAANQKLALQKAKEVAEVSPMSAANISIAAQQNSAKARSVWEQRASQLRLQNLRASCEALYSEMDPEERLRYASTRHVRPDMKTHMDRPLVVEPGRDGLRGPVGSKSKPEGTEATESADLPRRHHRHRDRDKTSATAPAGGEQDRTESTETGAREERARPRRSHSKETPGADTQVRCERSRRHHRRGSPEEATEREPRRHRAHRHAQDSSKEGTAPVLVPKGERRARHRGPRTGPREAENNEEPTRRHRARHKVPPTLQPPEREAAEKESNAVEGDKETRNHQPKEPHCDLEAIAVTGVGPLHMLPSTCLQKVDEQPEDADNQRNVTRMGSQPSDPSTTVHVPVTLTGPPGETPVVPSGNMNLEGQAEGKKEAEADDVLRRGPRPIVPYSSMFCLSPTNLLRRFCHYIVTMRYFEMVILVVIALSSIALAAEDPVRTDSFRNNALKYMDYIFTGVFTFEMVIKMIDLGLLLHPGAYFRDLWNILDFIVVSGALVAFAFSSFMGGSKGKDINTIKSLRVLRVLRPLKTIKRLPKLKAVFDCVVNSLKNVLNILIVYMLFMFIFAVIAVQLFKGKFFYCTDESKELERDCRGQYLDYEKEEVEAQPRQWKKYDFHYDNVLWALLTLFTVSTGEGWPMVLKHSVDATYEEQGPSPGFRMELSIFYVVYFVVFPFFFVNIFVALIIITFQEQGDKVMSECSLEKNERACIDFAISAKPLTRYMPQNKQSFQYKTWTFVVSPPFEYFIMAMIALNTVVLMMKFYDAPYEYELMLKCLNIVFTSMFSMECILKIIAFGVLNYFRDAWNVFDFVTVLGSITDILVTEIAETNNFINLSFLRLFRAARLIKLLRQGYTIRILLWTFVQSFKALPYVCLLIAMLFFIYAIIGMQVFGNIALDDDTSINRHNNFRTFLQALMLLFRSATGEAWHEIMLSCLGNRACDPHANASECGSDFAYFYFVSFIFLCSFLMLNLFVAVIMDNFEYLTRDSSILGPHHLDEFIRVWAEYDPAACGRISYNDMFEMLKHMSPPLGLGKKCPARVAYKRLVRMNMPISNEDMTVHFTSTLMALIRTALEIKLAPAGTKQHQCDAELRKEISSVWANLPQKTLDLLVPPHKPDEMTVGKVYAALMIFDFYKQNKTTRDQTHQAPGGLSQMGPVSLFHPLKATLEQTQPAVLRGARVFLRQKSATSLSNGGAIQTQESGIKESLSWGTQRTQDALYEARAPLERGHSAEIPVGQSGTLAVDVQMQNMTLRGPDGEPQPGLESQGRAASMPRLAAETQPAPNASPMKRSISTLAPRPHGTQLCSTVLDRPPPSQASHHHHHRCHRRRDKKQRSLEKGPSLSVDPEGAPSTAAGPGLPHGEGSTACRRDRKQERGRSQERRQPSSSSSEKQRFYSCDRFGSREPPQLMPSLSSHPTSPTAALEPAPHPQGSGSVNGSPLMSTSGASTPGRGGRRQLPQTPLTPRPSITYKTANSSPVHFAEGQSGLPAFSPGRLSRGLSEHNALLQKEPLSQPLAPGSRIGSDPYLGQRLDSEASAHTLPEDTLTFEEAVATNSGRSSRTSYVSSLTSQSHPLRRVPNGYHCTLGLSTGVRARHSYHHPDQDHWC

Target ORF information:

RefSeq Version	XM_017315146.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus calcium channel, voltage-dependent, N type, alpha 1B subunit (Cacna1b), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017315146.1

ORF Insert Sequence:

ATGGTCCGCT TCGGGGACGA GCTAGGCGGC CGCTATGGGG GCACCGGCGG CGGGGAGCGG 
GCTCGGGGTG GCGGGGCCGG CGGGGCGGGT GGCCCGGGCC AGGGGGGTCT GCCGCCGGGC 
CAGCGGGTCC TGTACAAGCA GTCCATTGCG CAGCGCGCAC GGACTATGGC CCTGTACAAC 
CCCATCCCAG TCAAGCAGAA CTGCTTCACC GTCAACCGCT CGCTCTTCGT CTTCAGCGAG 
GACAACGTCG TCCGCAAATA CGCTAAGCGC ATCACCGAAT GGCCGCCCTT CGAATACATG 
ATCCTGGCCA CCATCATCGC CAACTGCATT GTTCTGGCCC TGGAGCAGCA CCTCCCTGAT 
GGGGACAAGA CTCCCATGTC TGAGCGACTA GATGACACGG AGCCTTACTT CATCGGGATC 
TTTTGCTTCG AGGCGGGCAT CAAGATCATA GCCCTGGGCT TTGTTTTCCA CAAGGGCTCC 
TACCTTCGGA ACGGCTGGAA TGTCATGGAC TTCGTGGTGG TCCTCACGGG GATTCTCGCC 
ACAGCTGGAA CTGACTTTGA CCTGCGCACA CTGAGGGCTG TGCGTGTGCT TAGGCCCCTG 
AAGCTGGTGT CTGGAATTCC AAGCTTGCAG GTGGTGCTTA AGTCCATCAT GAAGGCCATG 
GTCCCGCTGC TGCAGATTGG GCTGCTGCTC TTCTTTGCCA TCCTCATGTT TGCCATCATC 
GGCCTCGAAT TCTATATGGG CAAATTCCAT AAGGCCTGTT TCCCCAACAG CACAGATACA 
GAGCCTGTGG GTGACTTTCC CTGTGGCAAA GATCCCCCTG CTCGTCAGTG TGATGGTGAC 
ACCGAATGCC GGGAGTACTG GCCAGGACCC AACTTTGGCA TCACCAATTT TGACAACATC 
CTGTTTGCCA TCTTGACAGT GTTCCAGTGT ATCACCATGG AGGGCTGGAC TGACATCCTC 
TACAATACAA ATGATGCGGC TGGCAACACA TGGAACTGGT TGTACTTCAT CCCCCTCATC 
ATCATTGGCT CCTTCTTCAT GCTCAACCTG GTGCTGGGTG TGCTTTCCGG AGAGTTTGCC 
AAGGAGCGGG AGCGAGTCGA GAACCGCCGC GCCTTCCTGA AGCTCCGCAG GCAGCAGCAG 
ATTGAGCGAG AGCTGAATGG GTACTTGGAG TGGATCTTCA AGGCAGAGGA AGTCATGTTG 
GCAGAGGAGG ACAAGAATGC AGAAGAGAAA TCCCCTTTGG ATGCAGTGTT GAAGAGAGCT 
GCCACCAAGA AGAGCCGAAA TGACCTCATC CATGCAGAAG AGGGGGAGGA CCGGTTTGTA 
GACCTCTGTG CAGTTGGGTC TCCATTTGCT CGTGCCAGCC TCAAGAGTGG GAAGACGGAG 
AGCTCATCGT ACTTCCGGAG AAAGGAGAAG ATGTTCCGGT TCTTTATCCG GCGTATGGTG 
AAAGCACAGA GCTTCTACTG GGTGGTACTG TGTGTGGTGG CCCTGAACAC ACTGTGTGTG 
GCCATGGTGC ACTATAATCA GCCTCAGCGG CTTACCACTG CACTGTACTT TGCAGAGTTT 
GTTTTCCTGG GTCTCTTCCT CACAGAGATG TCCCTGAAGA TGTATGGCCT AGGGCCCAGA 
AGTTACTTCA GGTCTTCCTT CAACTGCTTT GACTTTGGGG TGATTGTGGG GAGTATCTTT 
GAAGTAGTCT GGGCTGCCAT CAAGCCAGGA ACCTCCTTTG GAATCAGTGT GCTGCGGGCT 
CTGCGACTGC TGAGGATATT CAAAGTTACC AAGTATTGGA ACTCTCTGAG GAACCTGGTG 
GTTTCCCTCC TCAATTCCAT GAAGTCCATC ATCAGCCTTC TCTTCCTGCT TTTCCTCTTC 
ATCGTGGTCT TCGCTCTGTT GGGGATGCAG CTGTTTGGGG GACAGTTCAA CTTTCAAGAT 
GAGACTCCAA CCACCAATTT TGATACCTTC CCAGCTGCCA TCCTCACTGT CTTTCAGATC 
CTGACAGGAG AGGATTGGAA TGCCGTAATG TATCATGGGA TTGAGTCGCA AGGTGGAGTC 
AGCAAAGGCA TGTTTTCTTC CTTTTACTTC ATCGTCCTGA CACTGTTTGG AAACTACACC 
CTGCTGAATG TTTTTCTGGC CATTGCTGTG GACAACCTTG CCAATGCCCA GGAGTTGACC 
AAGGATGAAG AGGAGATGGA AGAAGCAGCC AATCAGAAAC TTGCTCTTCA GAAGGCCAAA 
GAAGTAGCTG AAGTCAGCCC CATGTCTGCT GCCAATATCT CCATCGCTGC GCAGCAGAAC 
TCGGCCAAGG CGCGCTCAGT ATGGGAGCAG CGGGCCAGTC AGCTAAGGCT CCAGAATCTG 
CGTGCCAGCT GTGAGGCATT GTACAGTGAG ATGGACCCTG AGGAGCGCCT GCGTTATGCC 
AGCACGCGCC ATGTGAGGCC AGACATGAAG ACACACATGG ACCGACCCCT AGTGGTGGAG 
CCTGGTCGAG ATGGCTTGCG GGGACCCGTT GGGAGCAAGT CAAAGCCTGA AGGCACGGAG 
GCCACAGAAA GCGCGGACCT ACCTCGCAGG CACCACCGGC ACCGTGATAG GGACAAGACC 
TCAGCCACAG CACCTGCTGG AGGCGAACAG GACAGGACAG AAAGCACCGA GACCGGGGCC 
CGGGAGGAAC GTGCGCGCCC TCGTCGAAGT CACAGCAAGG AGACTCCAGG GGCTGACACG 
CAAGTGCGCT GTGAGCGCAG TAGACGTCAC CACCGGCGCG GCTCCCCGGA GGAGGCCACT 
GAACGGGAGC CTCGGCGCCA CCGTGCCCAC CGGCATGCAC AGGACTCAAG CAAGGAGGGC 
ACGGCGCCGG TGCTTGTACC CAAGGGTGAG CGACGAGCAA GACACCGAGG CCCACGCACG 
GGTCCACGTG AGGCAGAGAA CAACGAGGAG CCCACACGCA GGCACCGTGC AAGGCATAAG 
GTGCCACCCA CACTGCAGCC CCCAGAGAGG GAGGCTGCAG AGAAGGAGAG CAACGCGGTG 
GAAGGGGATA AGGAAACCCG AAATCACCAG CCCAAGGAAC CTCACTGTGA CCTGGAGGCC 
ATTGCAGTTA CAGGTGTGGG CCCTCTGCAC ATGCTGCCCA GCACCTGTCT CCAGAAAGTG 
GACGAGCAAC CAGAGGATGC AGACAACCAG CGTAATGTCA CCCGGATGGG CAGTCAGCCC 
TCAGATCCCA GCACCACTGT GCATGTCCCA GTGACACTGA CAGGCCCTCC TGGGGAGACC 
CCTGTAGTTC CCAGTGGTAA CATGAACCTG GAAGGCCAAG CAGAGGGCAA GAAGGAGGCA 
GAGGCGGATG ATGTGCTGAG AAGAGGCCCC AGGCCCATCG TTCCCTACAG CTCCATGTTT 
TGTCTCAGCC CCACCAACCT GCTTCGTCGC TTCTGCCATT ACATTGTGAC CATGCGGTAC 
TTTGAGATGG TAATTCTTGT GGTCATTGCC TTGAGCAGCA TTGCCCTGGC TGCAGAGGAT 
CCTGTGCGGA CAGATTCATT CAGGAACAAC GCTTTAAAGT ACATGGATTA CATCTTTACA 
GGAGTCTTCA CCTTTGAAAT GGTCATAAAG ATGATAGACT TGGGCTTGCT GCTGCACCCT 
GGTGCCTACT TCCGGGACCT GTGGAACATT CTGGACTTCA TTGTTGTCAG TGGAGCCCTG 
GTGGCATTTG CGTTCTCGAG CTTCATGGGA GGATCCAAAG GGAAAGACAT CAATACCATC 
AAGTCTCTGA GAGTCCTGCG TGTCCTGAGG CCCCTCAAGA CCATCAAGCG GCTGCCTAAG 
CTCAAGGCTG TCTTTGACTG TGTGGTGAAC TCCCTGAAGA ACGTCTTGAA CATCCTGATT 
GTCTACATGC TCTTCATGTT CATATTTGCC GTCATTGCCG TCCAGCTCTT CAAAGGGAAG 
TTCTTTTACT GTACTGATGA ATCCAAGGAG CTGGAGAGGG ACTGCCGGGG TCAGTATTTG 
GATTATGAGA AGGAAGAAGT AGAAGCCCAG CCAAGGCAGT GGAAGAAATA TGACTTCCAC 
TATGACAATG TTCTCTGGGC CTTGTTGACG CTGTTCACAG TGTCCACGGG AGAGGGGTGG 
CCCATGGTGC TGAAACACTC TGTGGATGCC ACCTATGAGG AACAGGGGCC CAGTCCCGGG 
TTCCGGATGG AGCTCTCCAT CTTCTACGTG GTCTACTTTG TGGTCTTCCC TTTTTTCTTT 
GTCAACATCT TTGTGGCCTT GATCATTATC ACCTTCCAGG AACAGGGAGA TAAGGTGATG 
TCTGAATGCA GCTTAGAAAA GAATGAGAGG GCTTGCATTG ATTTTGCCAT CAGTGCCAAG 
CCCCTGACAC GGTACATGCC TCAAAACAAA CAGTCGTTCC AGTATAAGAC ATGGACATTC 
GTGGTCTCTC CACCCTTTGA GTACTTCATC ATGGCTATGA TAGCCCTCAA CACAGTGGTG 
CTGATGATGA AGTTCTATGA TGCACCTTAT GAGTACGAGC TGATGCTGAA ATGCCTGAAC 
ATTGTCTTCA CATCCATGTT CTCGATGGAG TGCATACTGA AGATCATCGC CTTTGGGGTA 
TTGAACTACT TCAGAGATGC CTGGAACGTC TTTGACTTTG TCACGGTTTT GGGAAGTATT 
ACTGATATTT TAGTAACAGA GATAGCGGAA ACGAACAACT TCATCAACCT AAGCTTCCTT 
CGCCTCTTCC GGGCGGCACG GCTGATCAAG CTGCTTCGCC AGGGCTACAC CATCCGCATC 
CTATTGTGGA CCTTCGTCCA GTCCTTTAAG GCGCTGCCCT ACGTGTGCCT CCTCATTGCC 
ATGCTGTTCT TCATCTACGC CATCATCGGC ATGCAGGTTT TTGGAAACAT TGCCCTTGAT 
GATGACACCA GTATCAACCG ACACAACAAC TTCCGGACAT TTCTGCAAGC CTTAATGCTA 
TTGTTCAGGA GTGCCACTGG GGAGGCCTGG CATGAGATCA TGCTGTCTTG TCTGGGCAAC 
CGGGCCTGTG ACCCACATGC CAACGCCAGT GAGTGCGGGA GCGACTTTGC CTATTTTTAT 
TTTGTCTCCT TCATCTTCCT CTGTTCCTTT CTGATGTTGA ACCTCTTTGT TGCTGTAATC 
ATGGACAATT TTGAGTACCT CACTCGGGAC TCTTCCATCC TAGGGCCTCA CCACTTAGAC 
GAATTCATTC GAGTCTGGGC TGAATACGAC CCAGCTGCGT GTGGGCGCAT CAGTTACAAT 
GACATGTTTG AGATGCTGAA ACACATGTCC CCACCTCTGG GGTTGGGGAA GAAATGCCCG 
GCTCGAGTTG CATACAAGCG CCTGGTTCGC ATGAACATGC CCATATCCAA TGAGGACATG 
ACGGTGCACT TTACGTCCAC ACTGATGGCC CTCATCCGGA CAGCACTGGA GATCAAGCTT 
GCCCCAGCGG GGACGAAGCA GCACCAGTGT GATGCTGAGC TGAGAAAAGA GATCTCTTCT 
GTGTGGGCTA ATCTGCCCCA GAAGACTCTG GACTTACTGG TACCACCCCA CAAACCTGAC 
GAGATGACAG TGGGGAAGGT CTATGCTGCT CTCATGATAT TTGACTTCTA CAAACAGAAC 
AAAACCACCA GAGATCAGAC TCACCAAGCT CCCGGAGGCC TGTCCCAGAT GGGTCCCGTT 
TCCCTGTTCC ACCCTCTGAA GGCCACCCTG GAACAGACAC AGCCCGCTGT GCTTCGAGGA 
GCTCGGGTTT TCCTTCGGCA AAAGAGTGCA ACTTCCCTCA GCAATGGGGG TGCCATACAA 
ACCCAGGAAA GTGGCATCAA GGAGTCGCTG TCCTGGGGCA CGCAGAGGAC CCAAGATGCA 
CTTTATGAGG CCAGAGCACC TCTAGAACGT GGCCATTCTG CAGAGATCCC TGTGGGGCAG 
TCAGGAACAC TGGCTGTGGA TGTCCAGATG CAGAACATGA CACTGAGAGG ACCAGATGGG 
GAGCCCCAGC CTGGGCTGGA AAGCCAAGGC AGAGCTGCCT CTATGCCACG CCTAGCGGCA 
GAAACACAGC CGGCCCCTAA TGCCAGCCCC ATGAAGCGCT CCATCTCCAC ACTGGCTCCA 
CGCCCACATG GGACTCAGCT TTGCAGCACA GTTCTGGACC GGCCTCCTCC TAGCCAGGCA 
TCACATCACC ACCACCACCG CTGCCACCGG CGCAGAGACA AGAAGCAAAG GTCCCTGGAA 
AAGGGGCCCA GCCTGTCTGT TGACCCAGAA GGTGCACCAA GCACTGCTGC AGGACCTGGT 
CTGCCCCATG GAGAAGGATC CACCGCCTGC CGGCGGGACC GTAAACAGGA GCGAGGCCGG 
TCCCAGGAGC GGAGGCAGCC CTCATCTTCC TCTTCAGAGA AGCAGCGCTT CTATTCCTGT 
GACCGCTTTG GGAGCCGGGA GCCCCCGCAA CTGATGCCCT CACTCAGTAG CCACCCCACA 
TCGCCAACAG CGGCGCTAGA GCCAGCACCC CACCCACAGG GCAGTGGTTC CGTTAATGGG 
AGCCCCTTGA TGTCAACATC CGGTGCTAGC ACCCCGGGCC GAGGTGGGCG GAGGCAGCTC 
CCCCAGACTC CTCTGACCCC ACGCCCCAGC ATCACCTACA AGACCGCCAA TTCCTCGCCT 
GTCCACTTTG CTGAGGGTCA GAGCGGCCTC CCAGCCTTCT CCCCTGGCCG TCTCAGCCGC 
GGCCTTTCTG AACACAATGC CCTGCTCCAG AAAGAGCCCC TGAGCCAGCC TCTAGCTCCT 
GGCTCCCGAA TTGGCTCTGA CCCTTACCTA GGGCAGCGTC TGGACAGTGA GGCCTCCGCC 
CACACCCTGC CTGAGGATAC ACTCACCTTT GAAGAGGCAG TGGCCACCAA CTCTGGCCGC 
TCCTCCAGGA CTTCCTATGT GTCCTCCCTC ACTTCCCAAT CCCACCCTCT CCGCCGTGTA 
CCCAATGGCT ATCACTGCAC TTTGGGACTC AGCACTGGCG TCCGGGCGCG GCACAGCTAC 
CACCACCCCG ATCAGGACCA CTGGTGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cacna1b ( XM_017315155.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017170644.1 Mus musculus calcium channel, voltage-dependent, N type, alpha 1B subunit (Cacna1b), transcript variant X18, mRNA.

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CloneID	OMu80054
Clone ID Related Accession (Same CDS sequence)	XM_017315155.1
Accession Version	XM_017315155.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5373bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	voltage-dependent N-type calcium channel subunit alpha-1B isoform X17
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTCCGCT TCGGGGACGA GCTAGGCGGC CGCTATGGGG GCACCGGCGG CGGGGAGCGG 
GCTCGGGGTG GCGGGGCCGG CGGGGCGGGT GGCCCGGGCC AGGGGGGTCT GCCGCCGGGC 
CAGCGGGTCC TGTACAAGCA GTCCATTGCG CAGCGCGCAC GGACTATGGC CCTGTACAAC 
CCCATCCCAG TCAAGCAGAA CTGCTTCACC GTCAACCGCT CGCTCTTCGT CTTCAGCGAG 
GACAACGTCG TCCGCAAATA CGCTAAGCGC ATCACCGAAT GGCCGCCCTT CGAATACATG 
ATCCTGGCCA CCATCATCGC CAACTGCATT GTTCTGGCCC TGGAGCAGCA CCTCCCTGAT 
GGGGACAAGA CTCCCATGTC TGAGCGACTA GATGACACGG AGCCTTACTT CATCGGGATC 
TTTTGCTTCG AGGCGGGCAT CAAGATCATA GCCCTGGGCT TTGTTTTCCA CAAGGGCTCC 
TACCTTCGGA ACGGCTGGAA TGTCATGGAC TTCGTGGTGG TCCTCACGGG GATTCTCGCC 
ACAGCTGGAA CTGACTTTGA CCTGCGCACA CTGAGGGCTG TGCGTGTGCT TAGGCCCCTG 
AAGCTGGTGT CTGGAATTCC AAGCTTGCAG GTGGTGCTTA AGTCCATCAT GAAGGCCATG 
GTCCCGCTGC TGCAGATTGG GCTGCTGCTC TTCTTTGCCA TCCTCATGTT TGCCATCATC 
GGCCTCGAAT TCTATATGGG CAAATTCCAT AAGGCCTGTT TCCCCAACAG CACAGATACA 
GAGCCTGTGG GTGACTTTCC CTGTGGCAAA GATCCCCCTG CTCGTCAGTG TGATGGTGAC 
ACCGAATGCC GGGAGTACTG GCCAGGACCC AACTTTGGCA TCACCAATTT TGACAACATC 
CTGTTTGCCA TCTTGACAGT GTTCCAGTGT ATCACCATGG AGGGCTGGAC TGACATCCTC 
TACAATACAA ATGATGCGGC TGGCAACACA TGGAACTGGT TGTACTTCAT CCCCCTCATC 
ATCATTGGCT CCTTCTTCAT GCTCAACCTG GTGCTGGGTG TGCTTTCCGG AGAGTTTGCC 
AAGGAGCGGG AGCGAGTCGA GAACCGCCGC GCCTTCCTGA AGCTCCGCAG GCAGCAGCAG 
ATTGAGCGAG AGCTGAATGG GTACTTGGAG TGGATCTTCA AGGCAGAGGA AGTCATGTTG 
GCAGAGGAGG ACAAGAATGC AGAAGAGAAA TCCCCTTTGG ATGCAGTGTT GAAGAGAGCT 
GCCACCAAGA AGAGCCGAAA TGACCTCATC CATGCAGAAG AGGGGGAGGA CCGGTTTGTA 
GACCTCTGTG CAGTTGGGTC TCCATTTGCT CGTGCCAGCC TCAAGAGTGG GAAGACGGAG 
AGCTCATCGT ACTTCCGGAG AAAGGAGAAG ATGTTCCGGT TCTTTATCCG GCGTATGGTG 
AAAGCACAGA GCTTCTACTG GGTGGTACTG TGTGTGGTGG CCCTGAACAC ACTGTGTGTG 
GCCATGGTGC ACTATAATCA GCCTCAGCGG CTTACCACTG CACTGTACTT TGCAGAGTTT 
GTTTTCCTGG GTCTCTTCCT CACAGAGATG TCCCTGAAGA TGTATGGCCT AGGGCCCAGA 
AGTTACTTCA GGTCTTCCTT CAACTGCTTT GACTTTGGGG TGATTGTGGG GAGTATCTTT 
GAAGTAGTCT GGGCTGCCAT CAAGCCAGGA ACCTCCTTTG GAATCAGTGT GCTGCGGGCT 
CTGCGACTGC TGAGGATATT CAAAGTTACC AAGTATTGGA ACTCTCTGAG GAACCTGGTG 
GTTTCCCTCC TCAATTCCAT GAAGTCCATC ATCAGCCTTC TCTTCCTGCT TTTCCTCTTC 
ATCGTGGTCT TCGCTCTGTT GGGGATGCAG CTGTTTGGGG GACAGTTCAA CTTTCAAGAT 
GAGACTCCAA CCACCAATTT TGATACCTTC CCAGCTGCCA TCCTCACTGT CTTTCAGATC 
CTGACAGGAG AGGATTGGAA TGCCGTAATG TATCATGGGA TTGAGTCGCA AGGTGGAGTC 
AGCAAAGGCA TGTTTTCTTC CTTTTACTTC ATCGTCCTGA CACTGTTTGG AAACTACACC 
CTGCTGAATG TTTTTCTGGC CATTGCTGTG GACAACCTTG CCAATGCCCA GGAGTTGACC 
AAGGATGAAG AGGAGATGGA AGAAGCAGCC AATCAGAAAC TTGCTCTTCA GAAGGCCAAA 
GAAGTAGCTG AAGTCAGCCC CATGTCTGCT GCCAATATCT CCATCGCTGC TTTTGTAAAG 
CAAACTCGAG GTACTGTATC TCGCAGCTCA TCTGTCTCCA GCGTAAACTC ACCCAGGCAG 
CAGAACTCGG CCAAGGCGCG CTCAGTATGG GAGCAGCGGG CCAGTCAGCT AAGGCTCCAG 
AATCTGCGTG CCAGCTGTGA GGCATTGTAC AGTGAGATGG ACCCTGAGGA GCGCCTGCGT 
TATGCCAGCA CGCGCCATGT GAGGCCAGAC ATGAAGACAC ACATGGACCG ACCCCTAGTG 
GTGGAGCCTG GTCGAGATGG CTTGCGGGGA CCCGTTGGGA GCAAGTCAAA GCCTGAAGGC 
ACGGAGGCCA CAGAAAGCGC GGACCTACCT CGCAGGCACC ACCGGCACCG TGATAGGGAC 
AAGACCTCAG CCACAGCACC TGCTGGAGGC GAACAGGACA GGACAGAAAG CACCGAGACC 
GGGGCCCGGG AGGAACGTGC GCGCCCTCGT CGAAGTCACA GCAAGGAGAC TCCAGGGGCT 
GACACGCAAG TGCGCTGTGA GCGCAGTAGA CGTCACCACC GGCGCGGCTC CCCGGAGGAG 
GCCACTGAAC GGGAGCCTCG GCGCCACCGT GCCCACCGGC ATGCACAGGA CTCAAGCAAG 
GAGGGCACGG CGCCGGTGCT TGTACCCAAG GGTGAGCGAC GAGCAAGACA CCGAGGCCCA 
CGCACGGGTC CACGTGAGGC AGAGAACAAC GAGGAGCCCA CACGCAGGCA CCGTGCAAGG 
CATAAGGTGC CACCCACACT GCAGCCCCCA GAGAGGGAGG CTGCAGAGAA GGAGAGCAAC 
GCGGTGGAAG GGGATAAGGA AACCCGAAAT CACCAGCCCA AGGAACCTCA CTGTGACCTG 
GAGGCCATTG CAGTTACAGG TGTGGGCCCT CTGCACATGC TGCCCAGCAC CTGTCTCCAG 
AAAGTGGACG AGCAACCAGA GGATGCAGAC AACCAGCGTA ATGTCACCCG GATGGGCAGT 
CAGCCCTCAG ATCCCAGCAC CACTGTGCAT GTCCCAGTGA CACTGACAGG CCCTCCTGGG 
GAGACCCCTG TAGTTCCCAG TGGTAACATG AACCTGGAAG GCCAAGCAGA GGGCAAGAAG 
GAGGCAGAGG CGGATGATGT GCTGAGAAGA GGCCCCAGGC CCATCGTTCC CTACAGCTCC 
ATGTTTTGTC TCAGCCCCAC CAACCTGCTT CGTCGCTTCT GCCATTACAT TGTGACCATG 
CGGTACTTTG AGATGGTAAT TCTTGTGGTC ATTGCCTTGA GCAGCATTGC CCTGGCTGCA 
GAGGATCCTG TGCGGACAGA TTCATTCAGG AACAACGCTT TAAAGTACAT GGATTACATC 
TTTACAGGAG TCTTCACCTT TGAAATGGTC ATAAAGATGA TAGACTTGGG CTTGCTGCTG 
CACCCTGGTG CCTACTTCCG GGACCTGTGG AACATTCTGG ACTTCATTGT TGTCAGTGGA 
GCCCTGGTGG CATTTGCGTT CTCAGGATCC AAAGGGAAAG ACATCAATAC CATCAAGTCT 
CTGAGAGTCC TGCGTGTCCT GAGGCCCCTC AAGACCATCA AGCGGCTGCC TAAGCTCAAG 
GCTGTCTTTG ACTGTGTGGT GAACTCCCTG AAGAACGTCT TGAACATCCT GATTGTCTAC 
ATGCTCTTCA TGTTCATATT TGCCGTCATT GCCGTCCAGC TCTTCAAAGG GAAGTTCTTT 
TACTGTACTG ATGAATCCAA GGAGCTGGAG AGGGACTGCC GGGGTCAGTA TTTGGATTAT 
GAGAAGGAAG AAGTAGAAGC CCAGCCAAGG CAGTGGAAGA AATATGACTT CCACTATGAC 
AATGTTCTCT GGGCCTTGTT GACGCTGTTC ACAGTGTCCA CGGGAGAGGG GTGGCCCATG 
GTGCTGAAAC ACTCTGTGGA TGCCACCTAT GAGGAACAGG GGCCCAGTCC CGGGTTCCGG 
ATGGAGCTCT CCATCTTCTA CGTGGTCTAC TTTGTGGTCT TCCCTTTTTT CTTTGTCAAC 
ATCTTTGTGG CCTTGATCAT TATCACCTTC CAGGAACAGG GAGATAAGGT GATGTCTGAA 
TGCAGCTTAG AAAAGAATGA GAGGGCTTGC ATTGATTTTG CCATCAGTGC CAAGCCCCTG 
ACACGGTACA TGCCTCAAAA CAAACAGTCG TTCCAGTATA AGACATGGAC ATTCGTGGTC 
TCTCCACCCT TTGAGTACTT CATCATGGCT ATGATAGCCC TCAACACAGT GGTGCTGATG 
ATGAAGTTCT ATGATGCACC TTATGAGTAC GAGCTGATGC TGAAATGCCT GAACATTGTC 
TTCACATCCA TGTTCTCGAT GGAGTGCATA CTGAAGATCA TCGCCTTTGG GGTATTGAAC 
TACTTCAGAG ATGCCTGGAA CGTCTTTGAC TTTGTCACGG TTTTGGGAAG TATTACTGAT 
ATTTTAGTAA CAGAGATAGC GAACAACTTC ATCAACCTAA GCTTCCTTCG CCTCTTCCGG 
GCGGCACGGC TGATCAAGCT GCTTCGCCAG GGCTACACCA TCCGCATCCT ATTGTGGACC 
TTCGTCCAGT CCTTTAAGGC GCTGCCCTAC GTGTGCCTCC TCATTGCCAT GCTGTTCTTC 
ATCTACGCCA TCATCGGCAT GCAGGTTTTT GGAAACATTG CCCTTGATGA TGACACCAGT 
ATCAACCGAC ACAACAACTT CCGGACATTT CTGCAAGCCT TAATGCTATT GTTCAGGAGT 
GCCACTGGGG AGGCCTGGCA TGAGATCATG CTGTCTTGTC TGGGCAACCG GGCCTGTGAC 
CCACATGCCA ACGCCAGTGA GTGCGGGAGC GACTTTGCCT ATTTTTATTT TGTCTCCTTC 
ATCTTCCTCT GTTCCTTTCT GATGTTGAAC CTCTTTGTTG CTGTAATCAT GGACAATTTT 
GAGTACCTCA CTCGGGACTC TTCCATCCTA GGGCCTCACC ACTTAGACGA ATTCATTCGA 
GTCTGGGCTG AATACGACCC AGCTGCGTGT TGCCGGATTC ATTATAAGGA TATGTACAGT 
TTGTTGCGTT GTATTGCGCC ACCCGTTGGC TTAGGAAAGA ACTGCCCTCG TAGGTTGGCC 
TACAAGGTTT GCAACTTCAG AGCCTCCATC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017170644.1
CDS	153..5525
Translation	MVRFGDELGGRYGGTGGGERARGGGAGGAGGPGQGGLPPGQRVLYKQSIAQRARTMALYNPIPVKQNCFTVNRSLFVFSEDNVVRKYAKRITEWPPFEYMILATIIANCIVLALEQHLPDGDKTPMSERLDDTEPYFIGIFCFEAGIKIIALGFVFHKGSYLRNGWNVMDFVVVLTGILATAGTDFDLRTLRAVRVLRPLKLVSGIPSLQVVLKSIMKAMVPLLQIGLLLFFAILMFAIIGLEFYMGKFHKACFPNSTDTEPVGDFPCGKDPPARQCDGDTECREYWPGPNFGITNFDNILFAILTVFQCITMEGWTDILYNTNDAAGNTWNWLYFIPLIIIGSFFMLNLVLGVLSGEFAKERERVENRRAFLKLRRQQQIERELNGYLEWIFKAEEVMLAEEDKNAEEKSPLDAVLKRAATKKSRNDLIHAEEGEDRFVDLCAVGSPFARASLKSGKTESSSYFRRKEKMFRFFIRRMVKAQSFYWVVLCVVALNTLCVAMVHYNQPQRLTTALYFAEFVFLGLFLTEMSLKMYGLGPRSYFRSSFNCFDFGVIVGSIFEVVWAAIKPGTSFGISVLRALRLLRIFKVTKYWNSLRNLVVSLLNSMKSIISLLFLLFLFIVVFALLGMQLFGGQFNFQDETPTTNFDTFPAAILTVFQILTGEDWNAVMYHGIESQGGVSKGMFSSFYFIVLTLFGNYTLLNVFLAIAVDNLANAQELTKDEEEMEEAANQKLALQKAKEVAEVSPMSAANISIAAFVKQTRGTVSRSSSVSSVNSPRQQNSAKARSVWEQRASQLRLQNLRASCEALYSEMDPEERLRYASTRHVRPDMKTHMDRPLVVEPGRDGLRGPVGSKSKPEGTEATESADLPRRHHRHRDRDKTSATAPAGGEQDRTESTETGAREERARPRRSHSKETPGADTQVRCERSRRHHRRGSPEEATEREPRRHRAHRHAQDSSKEGTAPVLVPKGERRARHRGPRTGPREAENNEEPTRRHRARHKVPPTLQPPEREAAEKESNAVEGDKETRNHQPKEPHCDLEAIAVTGVGPLHMLPSTCLQKVDEQPEDADNQRNVTRMGSQPSDPSTTVHVPVTLTGPPGETPVVPSGNMNLEGQAEGKKEAEADDVLRRGPRPIVPYSSMFCLSPTNLLRRFCHYIVTMRYFEMVILVVIALSSIALAAEDPVRTDSFRNNALKYMDYIFTGVFTFEMVIKMIDLGLLLHPGAYFRDLWNILDFIVVSGALVAFAFSGSKGKDINTIKSLRVLRVLRPLKTIKRLPKLKAVFDCVVNSLKNVLNILIVYMLFMFIFAVIAVQLFKGKFFYCTDESKELERDCRGQYLDYEKEEVEAQPRQWKKYDFHYDNVLWALLTLFTVSTGEGWPMVLKHSVDATYEEQGPSPGFRMELSIFYVVYFVVFPFFFVNIFVALIIITFQEQGDKVMSECSLEKNERACIDFAISAKPLTRYMPQNKQSFQYKTWTFVVSPPFEYFIMAMIALNTVVLMMKFYDAPYEYELMLKCLNIVFTSMFSMECILKIIAFGVLNYFRDAWNVFDFVTVLGSITDILVTEIANNFINLSFLRLFRAARLIKLLRQGYTIRILLWTFVQSFKALPYVCLLIAMLFFIYAIIGMQVFGNIALDDDTSINRHNNFRTFLQALMLLFRSATGEAWHEIMLSCLGNRACDPHANASECGSDFAYFYFVSFIFLCSFLMLNLFVAVIMDNFEYLTRDSSILGPHHLDEFIRVWAEYDPAACCRIHYKDMYSLLRCIAPPVGLGKNCPRRLAYKVCNFRASI

Target ORF information:

RefSeq Version	XM_017315155.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus calcium channel, voltage-dependent, N type, alpha 1B subunit (Cacna1b), transcript variant X18, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017315155.1

ORF Insert Sequence:

ATGGTCCGCT TCGGGGACGA GCTAGGCGGC CGCTATGGGG GCACCGGCGG CGGGGAGCGG 
GCTCGGGGTG GCGGGGCCGG CGGGGCGGGT GGCCCGGGCC AGGGGGGTCT GCCGCCGGGC 
CAGCGGGTCC TGTACAAGCA GTCCATTGCG CAGCGCGCAC GGACTATGGC CCTGTACAAC 
CCCATCCCAG TCAAGCAGAA CTGCTTCACC GTCAACCGCT CGCTCTTCGT CTTCAGCGAG 
GACAACGTCG TCCGCAAATA CGCTAAGCGC ATCACCGAAT GGCCGCCCTT CGAATACATG 
ATCCTGGCCA CCATCATCGC CAACTGCATT GTTCTGGCCC TGGAGCAGCA CCTCCCTGAT 
GGGGACAAGA CTCCCATGTC TGAGCGACTA GATGACACGG AGCCTTACTT CATCGGGATC 
TTTTGCTTCG AGGCGGGCAT CAAGATCATA GCCCTGGGCT TTGTTTTCCA CAAGGGCTCC 
TACCTTCGGA ACGGCTGGAA TGTCATGGAC TTCGTGGTGG TCCTCACGGG GATTCTCGCC 
ACAGCTGGAA CTGACTTTGA CCTGCGCACA CTGAGGGCTG TGCGTGTGCT TAGGCCCCTG 
AAGCTGGTGT CTGGAATTCC AAGCTTGCAG GTGGTGCTTA AGTCCATCAT GAAGGCCATG 
GTCCCGCTGC TGCAGATTGG GCTGCTGCTC TTCTTTGCCA TCCTCATGTT TGCCATCATC 
GGCCTCGAAT TCTATATGGG CAAATTCCAT AAGGCCTGTT TCCCCAACAG CACAGATACA 
GAGCCTGTGG GTGACTTTCC CTGTGGCAAA GATCCCCCTG CTCGTCAGTG TGATGGTGAC 
ACCGAATGCC GGGAGTACTG GCCAGGACCC AACTTTGGCA TCACCAATTT TGACAACATC 
CTGTTTGCCA TCTTGACAGT GTTCCAGTGT ATCACCATGG AGGGCTGGAC TGACATCCTC 
TACAATACAA ATGATGCGGC TGGCAACACA TGGAACTGGT TGTACTTCAT CCCCCTCATC 
ATCATTGGCT CCTTCTTCAT GCTCAACCTG GTGCTGGGTG TGCTTTCCGG AGAGTTTGCC 
AAGGAGCGGG AGCGAGTCGA GAACCGCCGC GCCTTCCTGA AGCTCCGCAG GCAGCAGCAG 
ATTGAGCGAG AGCTGAATGG GTACTTGGAG TGGATCTTCA AGGCAGAGGA AGTCATGTTG 
GCAGAGGAGG ACAAGAATGC AGAAGAGAAA TCCCCTTTGG ATGCAGTGTT GAAGAGAGCT 
GCCACCAAGA AGAGCCGAAA TGACCTCATC CATGCAGAAG AGGGGGAGGA CCGGTTTGTA 
GACCTCTGTG CAGTTGGGTC TCCATTTGCT CGTGCCAGCC TCAAGAGTGG GAAGACGGAG 
AGCTCATCGT ACTTCCGGAG AAAGGAGAAG ATGTTCCGGT TCTTTATCCG GCGTATGGTG 
AAAGCACAGA GCTTCTACTG GGTGGTACTG TGTGTGGTGG CCCTGAACAC ACTGTGTGTG 
GCCATGGTGC ACTATAATCA GCCTCAGCGG CTTACCACTG CACTGTACTT TGCAGAGTTT 
GTTTTCCTGG GTCTCTTCCT CACAGAGATG TCCCTGAAGA TGTATGGCCT AGGGCCCAGA 
AGTTACTTCA GGTCTTCCTT CAACTGCTTT GACTTTGGGG TGATTGTGGG GAGTATCTTT 
GAAGTAGTCT GGGCTGCCAT CAAGCCAGGA ACCTCCTTTG GAATCAGTGT GCTGCGGGCT 
CTGCGACTGC TGAGGATATT CAAAGTTACC AAGTATTGGA ACTCTCTGAG GAACCTGGTG 
GTTTCCCTCC TCAATTCCAT GAAGTCCATC ATCAGCCTTC TCTTCCTGCT TTTCCTCTTC 
ATCGTGGTCT TCGCTCTGTT GGGGATGCAG CTGTTTGGGG GACAGTTCAA CTTTCAAGAT 
GAGACTCCAA CCACCAATTT TGATACCTTC CCAGCTGCCA TCCTCACTGT CTTTCAGATC 
CTGACAGGAG AGGATTGGAA TGCCGTAATG TATCATGGGA TTGAGTCGCA AGGTGGAGTC 
AGCAAAGGCA TGTTTTCTTC CTTTTACTTC ATCGTCCTGA CACTGTTTGG AAACTACACC 
CTGCTGAATG TTTTTCTGGC CATTGCTGTG GACAACCTTG CCAATGCCCA GGAGTTGACC 
AAGGATGAAG AGGAGATGGA AGAAGCAGCC AATCAGAAAC TTGCTCTTCA GAAGGCCAAA 
GAAGTAGCTG AAGTCAGCCC CATGTCTGCT GCCAATATCT CCATCGCTGC TTTTGTAAAG 
CAAACTCGAG GTACTGTATC TCGCAGCTCA TCTGTCTCCA GCGTAAACTC ACCCAGGCAG 
CAGAACTCGG CCAAGGCGCG CTCAGTATGG GAGCAGCGGG CCAGTCAGCT AAGGCTCCAG 
AATCTGCGTG CCAGCTGTGA GGCATTGTAC AGTGAGATGG ACCCTGAGGA GCGCCTGCGT 
TATGCCAGCA CGCGCCATGT GAGGCCAGAC ATGAAGACAC ACATGGACCG ACCCCTAGTG 
GTGGAGCCTG GTCGAGATGG CTTGCGGGGA CCCGTTGGGA GCAAGTCAAA GCCTGAAGGC 
ACGGAGGCCA CAGAAAGCGC GGACCTACCT CGCAGGCACC ACCGGCACCG TGATAGGGAC 
AAGACCTCAG CCACAGCACC TGCTGGAGGC GAACAGGACA GGACAGAAAG CACCGAGACC 
GGGGCCCGGG AGGAACGTGC GCGCCCTCGT CGAAGTCACA GCAAGGAGAC TCCAGGGGCT 
GACACGCAAG TGCGCTGTGA GCGCAGTAGA CGTCACCACC GGCGCGGCTC CCCGGAGGAG 
GCCACTGAAC GGGAGCCTCG GCGCCACCGT GCCCACCGGC ATGCACAGGA CTCAAGCAAG 
GAGGGCACGG CGCCGGTGCT TGTACCCAAG GGTGAGCGAC GAGCAAGACA CCGAGGCCCA 
CGCACGGGTC CACGTGAGGC AGAGAACAAC GAGGAGCCCA CACGCAGGCA CCGTGCAAGG 
CATAAGGTGC CACCCACACT GCAGCCCCCA GAGAGGGAGG CTGCAGAGAA GGAGAGCAAC 
GCGGTGGAAG GGGATAAGGA AACCCGAAAT CACCAGCCCA AGGAACCTCA CTGTGACCTG 
GAGGCCATTG CAGTTACAGG TGTGGGCCCT CTGCACATGC TGCCCAGCAC CTGTCTCCAG 
AAAGTGGACG AGCAACCAGA GGATGCAGAC AACCAGCGTA ATGTCACCCG GATGGGCAGT 
CAGCCCTCAG ATCCCAGCAC CACTGTGCAT GTCCCAGTGA CACTGACAGG CCCTCCTGGG 
GAGACCCCTG TAGTTCCCAG TGGTAACATG AACCTGGAAG GCCAAGCAGA GGGCAAGAAG 
GAGGCAGAGG CGGATGATGT GCTGAGAAGA GGCCCCAGGC CCATCGTTCC CTACAGCTCC 
ATGTTTTGTC TCAGCCCCAC CAACCTGCTT CGTCGCTTCT GCCATTACAT TGTGACCATG 
CGGTACTTTG AGATGGTAAT TCTTGTGGTC ATTGCCTTGA GCAGCATTGC CCTGGCTGCA 
GAGGATCCTG TGCGGACAGA TTCATTCAGG AACAACGCTT TAAAGTACAT GGATTACATC 
TTTACAGGAG TCTTCACCTT TGAAATGGTC ATAAAGATGA TAGACTTGGG CTTGCTGCTG 
CACCCTGGTG CCTACTTCCG GGACCTGTGG AACATTCTGG ACTTCATTGT TGTCAGTGGA 
GCCCTGGTGG CATTTGCGTT CTCAGGATCC AAAGGGAAAG ACATCAATAC CATCAAGTCT 
CTGAGAGTCC TGCGTGTCCT GAGGCCCCTC AAGACCATCA AGCGGCTGCC TAAGCTCAAG 
GCTGTCTTTG ACTGTGTGGT GAACTCCCTG AAGAACGTCT TGAACATCCT GATTGTCTAC 
ATGCTCTTCA TGTTCATATT TGCCGTCATT GCCGTCCAGC TCTTCAAAGG GAAGTTCTTT 
TACTGTACTG ATGAATCCAA GGAGCTGGAG AGGGACTGCC GGGGTCAGTA TTTGGATTAT 
GAGAAGGAAG AAGTAGAAGC CCAGCCAAGG CAGTGGAAGA AATATGACTT CCACTATGAC 
AATGTTCTCT GGGCCTTGTT GACGCTGTTC ACAGTGTCCA CGGGAGAGGG GTGGCCCATG 
GTGCTGAAAC ACTCTGTGGA TGCCACCTAT GAGGAACAGG GGCCCAGTCC CGGGTTCCGG 
ATGGAGCTCT CCATCTTCTA CGTGGTCTAC TTTGTGGTCT TCCCTTTTTT CTTTGTCAAC 
ATCTTTGTGG CCTTGATCAT TATCACCTTC CAGGAACAGG GAGATAAGGT GATGTCTGAA 
TGCAGCTTAG AAAAGAATGA GAGGGCTTGC ATTGATTTTG CCATCAGTGC CAAGCCCCTG 
ACACGGTACA TGCCTCAAAA CAAACAGTCG TTCCAGTATA AGACATGGAC ATTCGTGGTC 
TCTCCACCCT TTGAGTACTT CATCATGGCT ATGATAGCCC TCAACACAGT GGTGCTGATG 
ATGAAGTTCT ATGATGCACC TTATGAGTAC GAGCTGATGC TGAAATGCCT GAACATTGTC 
TTCACATCCA TGTTCTCGAT GGAGTGCATA CTGAAGATCA TCGCCTTTGG GGTATTGAAC 
TACTTCAGAG ATGCCTGGAA CGTCTTTGAC TTTGTCACGG TTTTGGGAAG TATTACTGAT 
ATTTTAGTAA CAGAGATAGC GAACAACTTC ATCAACCTAA GCTTCCTTCG CCTCTTCCGG 
GCGGCACGGC TGATCAAGCT GCTTCGCCAG GGCTACACCA TCCGCATCCT ATTGTGGACC 
TTCGTCCAGT CCTTTAAGGC GCTGCCCTAC GTGTGCCTCC TCATTGCCAT GCTGTTCTTC 
ATCTACGCCA TCATCGGCAT GCAGGTTTTT GGAAACATTG CCCTTGATGA TGACACCAGT 
ATCAACCGAC ACAACAACTT CCGGACATTT CTGCAAGCCT TAATGCTATT GTTCAGGAGT 
GCCACTGGGG AGGCCTGGCA TGAGATCATG CTGTCTTGTC TGGGCAACCG GGCCTGTGAC 
CCACATGCCA ACGCCAGTGA GTGCGGGAGC GACTTTGCCT ATTTTTATTT TGTCTCCTTC 
ATCTTCCTCT GTTCCTTTCT GATGTTGAAC CTCTTTGTTG CTGTAATCAT GGACAATTTT 
GAGTACCTCA CTCGGGACTC TTCCATCCTA GGGCCTCACC ACTTAGACGA ATTCATTCGA 
GTCTGGGCTG AATACGACCC AGCTGCGTGT TGCCGGATTC ATTATAAGGA TATGTACAGT 
TTGTTGCGTT GTATTGCGCC ACCCGTTGGC TTAGGAAAGA ACTGCCCTCG TAGGTTGGCC 
TACAAGGTTT GCAACTTCAG AGCCTCCATC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cacna1b ( XM_017315156.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017170645.1 Mus musculus calcium channel, voltage-dependent, N type, alpha 1B subunit (Cacna1b), transcript variant X22, mRNA.

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CloneID	OMu80055
Clone ID Related Accession (Same CDS sequence)	XM_017315156.1
Accession Version	XM_017315156.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7035bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	voltage-dependent N-type calcium channel subunit alpha-1B isoform X20
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTCCGCT TCGGGGACGA GCTAGGCGGC CGCTATGGGG GCACCGGCGG CGGGGAGCGG 
GCTCGGGGTG GCGGGGCCGG CGGGGCGGGT GGCCCGGGCC AGGGGGGTCT GCCGCCGGGC 
CAGCGGGTCC TGTACAAGCA GTCCATTGCG CAGCGCGCAC GGACTATGGC CCTGTACAAC 
CCCATCCCAG TCAAGCAGAA CTGCTTCACC GTCAACCGCT CGCTCTTCGT CTTCAGCGAG 
GACAACGTCG TCCGCAAATA CGCTAAGCGC ATCACCGAAT GGCCGCCCTT CGAATACATG 
ATCCTGGCCA CCATCATCGC CAACTGCATT GTTCTGGCCC TGGAGCAGCA CCTCCCTGAT 
GGGGACAAGA CTCCCATGTC TGAGCGACTA GATGACACGG AGCCTTACTT CATCGGGATC 
TTTTGCTTCG AGGCGGGCAT CAAGATCATA GCCCTGGGCT TTGTTTTCCA CAAGGGCTCC 
TACCTTCGGA ACGGCTGGAA TGTCATGGAC TTCGTGGTGG TCCTCACGGG GATTCTCGCC 
ACAGCTGGAA CTGACTTTGA CCTGCGCACA CTGAGGGCTG TGCGTGTGCT TAGGCCCCTG 
AAGCTGGTGT CTGGAATTCC AAGCTTGCAG GTGGTGCTTA AGTCCATCAT GAAGGCCATG 
GTCCCGCTGC TGCAGATTGG GCTGCTGCTC TTCTTTGCCA TCCTCATGTT TGCCATCATC 
GGCCTCGAAT TCTATATGGG CAAATTCCAT AAGGCCTGTT TCCCCAACAG CACAGATACA 
GAGCCTGTGG GTGACTTTCC CTGTGGCAAA GATCCCCCTG CTCGTCAGTG TGATGGTGAC 
ACCGAATGCC GGGAGTACTG GCCAGGACCC AACTTTGGCA TCACCAATTT TGACAACATC 
CTGTTTGCCA TCTTGACAGT GTTCCAGTGT ATCACCATGG AGGGCTGGAC TGACATCCTC 
TACAATACAA ATGATGCGGC TGGCAACACA TGGAACTGGT TGTACTTCAT CCCCCTCATC 
ATCATTGGCT CCTTCTTCAT GCTCAACCTG GTGCTGGGTG TGCTTTCCGG AGAGTTTGCC 
AAGGAGCGGG AGCGAGTCGA GAACCGCCGC GCCTTCCTGA AGCTCCGCAG GCAGCAGCAG 
ATTGAGCGAG AGCTGAATGG GTACTTGGAG TGGATCTTCA AGGCAGAGGA AGTCATGTTG 
GCAGAGGAGG ACAAGAATGC AGAAGAGAAA TCCCCTTTGG ATGTGTTGAA GAGAGCTGCC 
ACCAAGAAGA GCCGAAATGA CCTCATCCAT GCAGAAGAGG GGGAGGACCG GTTTGTAGAC 
CTCTGTGCAG TTGGGTCTCC ATTTGCTCGT GCCAGCCTCA AGAGTGGGAA GACGGAGAGC 
TCATCGTACT TCCGGAGAAA GGAGAAGATG TTCCGGTTCT TTATCCGGCG TATGGTGAAA 
GCACAGAGCT TCTACTGGGT GGTACTGTGT GTGGTGGCCC TGAACACACT GTGTGTGGCC 
ATGGTGCACT ATAATCAGCC TCAGCGGCTT ACCACTGCAC TGTACTTTGC AGAGTTTGTT 
TTCCTGGGTC TCTTCCTCAC AGAGATGTCC CTGAAGATGT ATGGCCTAGG GCCCAGAAGT 
TACTTCAGGT CTTCCTTCAA CTGCTTTGAC TTTGGGGTGA TTGTGGGGAG TATCTTTGAA 
GTAGTCTGGG CTGCCATCAA GCCAGGAACC TCCTTTGGAA TCAGTGTGCT GCGGGCTCTG 
CGACTGCTGA GGATATTCAA AGTTACCAAG TATTGGAACT CTCTGAGGAA CCTGGTGGTT 
TCCCTCCTCA ATTCCATGAA GTCCATCATC AGCCTTCTCT TCCTGCTTTT CCTCTTCATC 
GTGGTCTTCG CTCTGTTGGG GATGCAGCTG TTTGGGGGAC AGTTCAACTT TCAAGATGAG 
ACTCCAACCA CCAATTTTGA TACCTTCCCA GCTGCCATCC TCACTGTCTT TCAGATCCTG 
ACAGGAGAGG ATTGGAATGC CGTAATGTAT CATGGGATTG AGTCGCAAGG TGGAGTCAGC 
AAAGGCATGT TTTCTTCCTT TTACTTCATC GTCCTGACAC TGTTTGGAAA CTACACCCTG 
CTGAATGTTT TTCTGGCCAT TGCTGTGGAC AACCTTGCCA ATGCCCAGGA GTTGACCAAG 
GATGAAGAGG AGATGGAAGA AGCAGCCAAT CAGAAACTTG CTCTTCAGAA GGCCAAAGAA 
GTAGCTGAAG TCAGCCCCAT GTCTGCTGCC AATATCTCCA TCGCTGCTTT TGTAAAGCAA 
ACTCGAGGTA CTGTATCTCG CAGCTCATCT GTCTCCAGCG TAAACTCACC CAGGCAGCAG 
AACTCGGCCA AGGCGCGCTC AGTATGGGAG CAGCGGGCCA GTCAGCTAAG GCTCCAGAAT 
CTGCGTGCCA GCTGTGAGGC ATTGTACAGT GAGATGGACC CTGAGGAGCG CCTGCGTTAT 
GCCAGCACGC GCCATGTGAG GCCAGACATG AAGACACACA TGGACCGACC CCTAGTGGTG 
GAGCCTGGTC GAGATGGCTT GCGGGGACCC GTTGGGAGCA AGTCAAAGCC TGAAGGCACG 
GAGGCCACAG AAAGCGCGGA CCTACCTCGC AGGCACCACC GGCACCGTGA TAGGGACAAG 
ACCTCAGCCA CAGCACCTGC TGGAGGCGAA CAGGACAGGA CAGAAAGCAC CGAGACCGGG 
GCCCGGGAGG AACGTGCGCG CCCTCGTCGA AGTCACAGCA AGGAGACTCC AGGGGCTGAC 
ACGCAAGTGC GCTGTGAGCG CAGTAGACGT CACCACCGGC GCGGCTCCCC GGAGGAGGCC 
ACTGAACGGG AGCCTCGGCG CCACCGTGCC CACCGGCATG CACAGGACTC AAGCAAGGAG 
GGCACGGCGC CGGTGCTTGT ACCCAAGGGT GAGCGACGAG CAAGACACCG AGGCCCACGC 
ACGGGTCCAC GTGAGGCAGA GAACAACGAG GAGCCCACAC GCAGGCACCG TGCAAGGCAT 
AAGGTGCCAC CCACACTGCA GCCCCCAGAG AGGGAGGCTG CAGAGAAGGA GAGCAACGCG 
GTGGAAGGGG ATAAGGAAAC CCGAAATCAC CAGCCCAAGG AACCTCACTG TGACCTGGAG 
GCCATTGCAG TTACAGGTGT GGGCCCTCTG CACATGCTGC CCAGCACCTG TCTCCAGAAA 
GTGGACGAGC AACCAGAGGA TGCAGACAAC CAGCGTAATG TCACCCGGAT GGGCAGTCAG 
CCCTCAGATC CCAGCACCAC TGTGCATGTC CCAGTGACAC TGACAGGCCC TCCTGGGGAG 
ACCCCTGTAG TTCCCAGTGG TAACATGAAC CTGGAAGGCC AAGCAGAGGG CAAGAAGGAG 
GCAGAGGCGG ATGATGTGCT GAGAAGAGGC CCCAGGCCCA TCGTTCCCTA CAGCTCCATG 
TTTTGTCTCA GCCCCACCAA CCTGCTTCGT CGCTTCTGCC ATTACATTGT GACCATGCGG 
TACTTTGAGA TGGTAATTCT TGTGGTCATT GCCTTGAGCA GCATTGCCCT GGCTGCAGAG 
GATCCTGTGC GGACAGATTC ATTCAGGAAC AACGCTTTAA AGTACATGGA TTACATCTTT 
ACAGGAGTCT TCACCTTTGA AATGGTCATA AAGATGATAG ACTTGGGCTT GCTGCTGCAC 
CCTGGTGCCT ACTTCCGGGA CCTGTGGAAC ATTCTGGACT TCATTGTTGT CAGTGGAGCC 
CTGGTGGCAT TTGCGTTCTC AGGATCCAAA GGGAAAGACA TCAATACCAT CAAGTCTCTG 
AGAGTCCTGC GTGTCCTGAG GCCCCTCAAG ACCATCAAGC GGCTGCCTAA GCTCAAGGCT 
GTCTTTGACT GTGTGGTGAA CTCCCTGAAG AACGTCTTGA ACATCCTGAT TGTCTACATG 
CTCTTCATGT TCATATTTGC CGTCATTGCC GTCCAGCTCT TCAAAGGGAA GTTCTTTTAC 
TGTACTGATG AATCCAAGGA GCTGGAGAGG GACTGCCGGG GTCAGTATTT GGATTATGAG 
AAGGAAGAAG TAGAAGCCCA GCCAAGGCAG TGGAAGAAAT ATGACTTCCA CTATGACAAT 
GTTCTCTGGG CCTTGTTGAC GCTGTTCACA GTGTCCACGG GAGAGGGGTG GCCCATGGTG 
CTGAAACACT CTGTGGATGC CACCTATGAG GAACAGGGGC CCAGTCCCGG GTTCCGGATG 
GAGCTCTCCA TCTTCTACGT GGTCTACTTT GTGGTCTTCC CTTTTTTCTT TGTCAACATC 
TTTGTGGCCT TGATCATTAT CACCTTCCAG GAACAGGGAG ATAAGGTGAT GTCTGAATGC 
AGCTTAGAAA AGAATGAGAG GGCTTGCATT GATTTTGCCA TCAGTGCCAA GCCCCTGACA 
CGGTACATGC CTCAAAACAA ACAGTCGTTC CAGTATAAGA CATGGACATT CGTGGTCTCT 
CCACCCTTTG AGTACTTCAT CATGGCTATG ATAGCCCTCA ACACAGTGGT GCTGATGATG 
AAGTTCTATG ATGCACCTTA TGAGTACGAG CTGATGCTGA AATGCCTGAA CATTGTCTTC 
ACATCCATGT TCTCGATGGA GTGCATACTG AAGATCATCG CCTTTGGGGT ATTGAACTAC 
TTCAGAGATG CCTGGAACGT CTTTGACTTT GTCACGGTTT TGGGAAGTAT TACTGATATT 
TTAGTAACAG AGATAGCGAA CAACTTCATC AACCTAAGCT TCCTTCGCCT CTTCCGGGCG 
GCACGGCTGA TCAAGCTGCT TCGCCAGGGC TACACCATCC GCATCCTATT GTGGACCTTC 
GTCCAGTCCT TTAAGGCGCT GCCCTACGTG TGCCTCCTCA TTGCCATGCT GTTCTTCATC 
TACGCCATCA TCGGCATGCA GGTTTTTGGA AACATTGCCC TTGATGATGA CACCAGTATC 
AACCGACACA ACAACTTCCG GACATTTCTG CAAGCCTTAA TGCTATTGTT CAGGAGTGCC 
ACTGGGGAGG CCTGGCATGA GATCATGCTG TCTTGTCTGG GCAACCGGGC CTGTGACCCA 
CATGCCAACG CCAGTGAGTG CGGGAGCGAC TTTGCCTATT TTTATTTTGT CTCCTTCATC 
TTCCTCTGTT CCTTTCTGAT GTTGAACCTC TTTGTTGCTG TAATCATGGA CAATTTTGAG 
TACCTCACTC GGGACTCTTC CATCCTAGGG CCTCACCACT TAGACGAATT CATTCGAGTC 
TGGGCTGAAT ACGACCCAGC TGCGTGTGGG CGCATCAGTT ACAATGACAT GTTTGAGATG 
CTGAAACACA TGTCCCCACC TCTGGGGTTG GGGAAGAAAT GCCCGGCTCG AGTTGCATAC 
AAGCGCCTGG TTCGCATGAA CATGCCCATA TCCAATGAGG ACATGACGGT GCACTTTACG 
TCCACACTGA TGGCCCTCAT CCGGACAGCA CTGGAGATCA AGCTTGCCCC AGCGGGGACG 
AAGCAGCACC AGTGTGATGC TGAGCTGAGA AAAGAGATCT CTTCTGTGTG GGCTAATCTG 
CCCCAGAAGA CTCTGGACTT ACTGGTACCA CCCCACAAAC CTGACGAGAT GACAGTGGGG 
AAGGTCTATG CTGCTCTCAT GATATTTGAC TTCTACAAAC AGAACAAAAC CACCAGAGAT 
CAGACTCACC AAGCTCCCGG AGGCCTGTCC CAGATGGGTC CCGTTTCCCT GTTCCACCCT 
CTGAAGGCCA CCCTGGAACA GACACAGCCC GCTGTGCTTC GAGGAGCTCG GGTTTTCCTT 
CGGCAAAAGA GTGCAACTTC CCTCAGCAAT GGGGGTGCCA TACAAACCCA GGAAAGTGGC 
ATCAAGGAGT CGCTGTCCTG GGGCACGCAG AGGACCCAAG ATGCACTTTA TGAGGCCAGA 
GCACCTCTAG AACGTGGCCA TTCTGCAGAG ATCCCTGTGG GGCAGTCAGG AACACTGGCT 
GTGGATGTCC AGATGCAGAA CATGACACTG AGAGGACCAG ATGGGGAGCC CCAGCCTGGG 
CTGGAAAGCC AAGGCAGAGC TGCCTCTATG CCACGCCTAG CGGCAGAAAC ACAGCCGGCC 
CCTAATGCCA GCCCCATGAA GCGCTCCATC TCCACACTGG CTCCACGCCC ACATGGGACT 
CAGCTTTGCA GCACAGTTCT GGACCGGCCT CCTCCTAGCC AGGCATCACA TCACCACCAC 
CACCGCTGCC ACCGGCGCAG AGACAAGAAG CAAAGGTCCC TGGAAAAGGG GCCCAGCCTG 
TCTGTTGACC CAGAAGGTGC ACCAAGCACT GCTGCAGGAC CTGGTCTGCC CCATGGAGAA 
GGATCCACCG CCTGCCGGCG GGACCGTAAA CAGGAGCGAG GCCGGTCCCA GGAGCGGAGG 
CAGCCCTCAT CTTCCTCTTC AGAGAAGCAG CGCTTCTATT CCTGTGACCG CTTTGGGAGC 
CGGGAGCCCC CGCAACTGAT GCCCTCACTC AGTAGCCACC CCACATCGCC AACAGCGGCG 
CTAGAGCCAG CACCCCACCC ACAGGGCAGT GGTTCCGTTA ATGGGAGCCC CTTGATGTCA 
ACATCCGGTG CTAGCACCCC GGGCCGAGGT GGGCGGAGGC AGCTCCCCCA GACTCCTCTG 
ACCCCACGCC CCAGCATCAC CTACAAGACC GCCAATTCCT CGCCTGTCCA CTTTGCTGAG 
GGTCAGAGCG GCCTCCCAGC CTTCTCCCCT GGCCGTCTCA GCCGCGGCCT TTCTGAACAC 
AATGCCCTGC TCCAGAAAGA GCCCCTGAGC CAGCCTCTAG CTCCTGGCTC CCGAATTGGC 
TCTGACCCTT ACCTAGGGCA GCGTCTGGAC AGTGAGGCCT CCGCCCACAC CCTGCCTGAG 
GATACACTCA CCTTTGAAGA GGCAGTGGCC ACCAACTCTG GCCGCTCCTC CAGGACTTCC 
TATGTGTCCT CCCTCACTTC CCAATCCCAC CCTCTCCGCC GTGTACCCAA TGGCTATCAC 
TGCACTTTGG GACTCAGCAC TGGCGTCCGG GCGCGGCACA GCTACCACCA CCCCGATCAG 
GACCACTGGT GCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017170645.1
CDS	146..7180
Translation	MVRFGDELGGRYGGTGGGERARGGGAGGAGGPGQGGLPPGQRVLYKQSIAQRARTMALYNPIPVKQNCFTVNRSLFVFSEDNVVRKYAKRITEWPPFEYMILATIIANCIVLALEQHLPDGDKTPMSERLDDTEPYFIGIFCFEAGIKIIALGFVFHKGSYLRNGWNVMDFVVVLTGILATAGTDFDLRTLRAVRVLRPLKLVSGIPSLQVVLKSIMKAMVPLLQIGLLLFFAILMFAIIGLEFYMGKFHKACFPNSTDTEPVGDFPCGKDPPARQCDGDTECREYWPGPNFGITNFDNILFAILTVFQCITMEGWTDILYNTNDAAGNTWNWLYFIPLIIIGSFFMLNLVLGVLSGEFAKERERVENRRAFLKLRRQQQIERELNGYLEWIFKAEEVMLAEEDKNAEEKSPLDVLKRAATKKSRNDLIHAEEGEDRFVDLCAVGSPFARASLKSGKTESSSYFRRKEKMFRFFIRRMVKAQSFYWVVLCVVALNTLCVAMVHYNQPQRLTTALYFAEFVFLGLFLTEMSLKMYGLGPRSYFRSSFNCFDFGVIVGSIFEVVWAAIKPGTSFGISVLRALRLLRIFKVTKYWNSLRNLVVSLLNSMKSIISLLFLLFLFIVVFALLGMQLFGGQFNFQDETPTTNFDTFPAAILTVFQILTGEDWNAVMYHGIESQGGVSKGMFSSFYFIVLTLFGNYTLLNVFLAIAVDNLANAQELTKDEEEMEEAANQKLALQKAKEVAEVSPMSAANISIAAFVKQTRGTVSRSSSVSSVNSPRQQNSAKARSVWEQRASQLRLQNLRASCEALYSEMDPEERLRYASTRHVRPDMKTHMDRPLVVEPGRDGLRGPVGSKSKPEGTEATESADLPRRHHRHRDRDKTSATAPAGGEQDRTESTETGAREERARPRRSHSKETPGADTQVRCERSRRHHRRGSPEEATEREPRRHRAHRHAQDSSKEGTAPVLVPKGERRARHRGPRTGPREAENNEEPTRRHRARHKVPPTLQPPEREAAEKESNAVEGDKETRNHQPKEPHCDLEAIAVTGVGPLHMLPSTCLQKVDEQPEDADNQRNVTRMGSQPSDPSTTVHVPVTLTGPPGETPVVPSGNMNLEGQAEGKKEAEADDVLRRGPRPIVPYSSMFCLSPTNLLRRFCHYIVTMRYFEMVILVVIALSSIALAAEDPVRTDSFRNNALKYMDYIFTGVFTFEMVIKMIDLGLLLHPGAYFRDLWNILDFIVVSGALVAFAFSGSKGKDINTIKSLRVLRVLRPLKTIKRLPKLKAVFDCVVNSLKNVLNILIVYMLFMFIFAVIAVQLFKGKFFYCTDESKELERDCRGQYLDYEKEEVEAQPRQWKKYDFHYDNVLWALLTLFTVSTGEGWPMVLKHSVDATYEEQGPSPGFRMELSIFYVVYFVVFPFFFVNIFVALIIITFQEQGDKVMSECSLEKNERACIDFAISAKPLTRYMPQNKQSFQYKTWTFVVSPPFEYFIMAMIALNTVVLMMKFYDAPYEYELMLKCLNIVFTSMFSMECILKIIAFGVLNYFRDAWNVFDFVTVLGSITDILVTEIANNFINLSFLRLFRAARLIKLLRQGYTIRILLWTFVQSFKALPYVCLLIAMLFFIYAIIGMQVFGNIALDDDTSINRHNNFRTFLQALMLLFRSATGEAWHEIMLSCLGNRACDPHANASECGSDFAYFYFVSFIFLCSFLMLNLFVAVIMDNFEYLTRDSSILGPHHLDEFIRVWAEYDPAACGRISYNDMFEMLKHMSPPLGLGKKCPARVAYKRLVRMNMPISNEDMTVHFTSTLMALIRTALEIKLAPAGTKQHQCDAELRKEISSVWANLPQKTLDLLVPPHKPDEMTVGKVYAALMIFDFYKQNKTTRDQTHQAPGGLSQMGPVSLFHPLKATLEQTQPAVLRGARVFLRQKSATSLSNGGAIQTQESGIKESLSWGTQRTQDALYEARAPLERGHSAEIPVGQSGTLAVDVQMQNMTLRGPDGEPQPGLESQGRAASMPRLAAETQPAPNASPMKRSISTLAPRPHGTQLCSTVLDRPPPSQASHHHHHRCHRRRDKKQRSLEKGPSLSVDPEGAPSTAAGPGLPHGEGSTACRRDRKQERGRSQERRQPSSSSSEKQRFYSCDRFGSREPPQLMPSLSSHPTSPTAALEPAPHPQGSGSVNGSPLMSTSGASTPGRGGRRQLPQTPLTPRPSITYKTANSSPVHFAEGQSGLPAFSPGRLSRGLSEHNALLQKEPLSQPLAPGSRIGSDPYLGQRLDSEASAHTLPEDTLTFEEAVATNSGRSSRTSYVSSLTSQSHPLRRVPNGYHCTLGLSTGVRARHSYHHPDQDHWC

Target ORF information:

RefSeq Version	XM_017315156.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus calcium channel, voltage-dependent, N type, alpha 1B subunit (Cacna1b), transcript variant X22, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017315156.1

ORF Insert Sequence:

ATGGTCCGCT TCGGGGACGA GCTAGGCGGC CGCTATGGGG GCACCGGCGG CGGGGAGCGG 
GCTCGGGGTG GCGGGGCCGG CGGGGCGGGT GGCCCGGGCC AGGGGGGTCT GCCGCCGGGC 
CAGCGGGTCC TGTACAAGCA GTCCATTGCG CAGCGCGCAC GGACTATGGC CCTGTACAAC 
CCCATCCCAG TCAAGCAGAA CTGCTTCACC GTCAACCGCT CGCTCTTCGT CTTCAGCGAG 
GACAACGTCG TCCGCAAATA CGCTAAGCGC ATCACCGAAT GGCCGCCCTT CGAATACATG 
ATCCTGGCCA CCATCATCGC CAACTGCATT GTTCTGGCCC TGGAGCAGCA CCTCCCTGAT 
GGGGACAAGA CTCCCATGTC TGAGCGACTA GATGACACGG AGCCTTACTT CATCGGGATC 
TTTTGCTTCG AGGCGGGCAT CAAGATCATA GCCCTGGGCT TTGTTTTCCA CAAGGGCTCC 
TACCTTCGGA ACGGCTGGAA TGTCATGGAC TTCGTGGTGG TCCTCACGGG GATTCTCGCC 
ACAGCTGGAA CTGACTTTGA CCTGCGCACA CTGAGGGCTG TGCGTGTGCT TAGGCCCCTG 
AAGCTGGTGT CTGGAATTCC AAGCTTGCAG GTGGTGCTTA AGTCCATCAT GAAGGCCATG 
GTCCCGCTGC TGCAGATTGG GCTGCTGCTC TTCTTTGCCA TCCTCATGTT TGCCATCATC 
GGCCTCGAAT TCTATATGGG CAAATTCCAT AAGGCCTGTT TCCCCAACAG CACAGATACA 
GAGCCTGTGG GTGACTTTCC CTGTGGCAAA GATCCCCCTG CTCGTCAGTG TGATGGTGAC 
ACCGAATGCC GGGAGTACTG GCCAGGACCC AACTTTGGCA TCACCAATTT TGACAACATC 
CTGTTTGCCA TCTTGACAGT GTTCCAGTGT ATCACCATGG AGGGCTGGAC TGACATCCTC 
TACAATACAA ATGATGCGGC TGGCAACACA TGGAACTGGT TGTACTTCAT CCCCCTCATC 
ATCATTGGCT CCTTCTTCAT GCTCAACCTG GTGCTGGGTG TGCTTTCCGG AGAGTTTGCC 
AAGGAGCGGG AGCGAGTCGA GAACCGCCGC GCCTTCCTGA AGCTCCGCAG GCAGCAGCAG 
ATTGAGCGAG AGCTGAATGG GTACTTGGAG TGGATCTTCA AGGCAGAGGA AGTCATGTTG 
GCAGAGGAGG ACAAGAATGC AGAAGAGAAA TCCCCTTTGG ATGTGTTGAA GAGAGCTGCC 
ACCAAGAAGA GCCGAAATGA CCTCATCCAT GCAGAAGAGG GGGAGGACCG GTTTGTAGAC 
CTCTGTGCAG TTGGGTCTCC ATTTGCTCGT GCCAGCCTCA AGAGTGGGAA GACGGAGAGC 
TCATCGTACT TCCGGAGAAA GGAGAAGATG TTCCGGTTCT TTATCCGGCG TATGGTGAAA 
GCACAGAGCT TCTACTGGGT GGTACTGTGT GTGGTGGCCC TGAACACACT GTGTGTGGCC 
ATGGTGCACT ATAATCAGCC TCAGCGGCTT ACCACTGCAC TGTACTTTGC AGAGTTTGTT 
TTCCTGGGTC TCTTCCTCAC AGAGATGTCC CTGAAGATGT ATGGCCTAGG GCCCAGAAGT 
TACTTCAGGT CTTCCTTCAA CTGCTTTGAC TTTGGGGTGA TTGTGGGGAG TATCTTTGAA 
GTAGTCTGGG CTGCCATCAA GCCAGGAACC TCCTTTGGAA TCAGTGTGCT GCGGGCTCTG 
CGACTGCTGA GGATATTCAA AGTTACCAAG TATTGGAACT CTCTGAGGAA CCTGGTGGTT 
TCCCTCCTCA ATTCCATGAA GTCCATCATC AGCCTTCTCT TCCTGCTTTT CCTCTTCATC 
GTGGTCTTCG CTCTGTTGGG GATGCAGCTG TTTGGGGGAC AGTTCAACTT TCAAGATGAG 
ACTCCAACCA CCAATTTTGA TACCTTCCCA GCTGCCATCC TCACTGTCTT TCAGATCCTG 
ACAGGAGAGG ATTGGAATGC CGTAATGTAT CATGGGATTG AGTCGCAAGG TGGAGTCAGC 
AAAGGCATGT TTTCTTCCTT TTACTTCATC GTCCTGACAC TGTTTGGAAA CTACACCCTG 
CTGAATGTTT TTCTGGCCAT TGCTGTGGAC AACCTTGCCA ATGCCCAGGA GTTGACCAAG 
GATGAAGAGG AGATGGAAGA AGCAGCCAAT CAGAAACTTG CTCTTCAGAA GGCCAAAGAA 
GTAGCTGAAG TCAGCCCCAT GTCTGCTGCC AATATCTCCA TCGCTGCTTT TGTAAAGCAA 
ACTCGAGGTA CTGTATCTCG CAGCTCATCT GTCTCCAGCG TAAACTCACC CAGGCAGCAG 
AACTCGGCCA AGGCGCGCTC AGTATGGGAG CAGCGGGCCA GTCAGCTAAG GCTCCAGAAT 
CTGCGTGCCA GCTGTGAGGC ATTGTACAGT GAGATGGACC CTGAGGAGCG CCTGCGTTAT 
GCCAGCACGC GCCATGTGAG GCCAGACATG AAGACACACA TGGACCGACC CCTAGTGGTG 
GAGCCTGGTC GAGATGGCTT GCGGGGACCC GTTGGGAGCA AGTCAAAGCC TGAAGGCACG 
GAGGCCACAG AAAGCGCGGA CCTACCTCGC AGGCACCACC GGCACCGTGA TAGGGACAAG 
ACCTCAGCCA CAGCACCTGC TGGAGGCGAA CAGGACAGGA CAGAAAGCAC CGAGACCGGG 
GCCCGGGAGG AACGTGCGCG CCCTCGTCGA AGTCACAGCA AGGAGACTCC AGGGGCTGAC 
ACGCAAGTGC GCTGTGAGCG CAGTAGACGT CACCACCGGC GCGGCTCCCC GGAGGAGGCC 
ACTGAACGGG AGCCTCGGCG CCACCGTGCC CACCGGCATG CACAGGACTC AAGCAAGGAG 
GGCACGGCGC CGGTGCTTGT ACCCAAGGGT GAGCGACGAG CAAGACACCG AGGCCCACGC 
ACGGGTCCAC GTGAGGCAGA GAACAACGAG GAGCCCACAC GCAGGCACCG TGCAAGGCAT 
AAGGTGCCAC CCACACTGCA GCCCCCAGAG AGGGAGGCTG CAGAGAAGGA GAGCAACGCG 
GTGGAAGGGG ATAAGGAAAC CCGAAATCAC CAGCCCAAGG AACCTCACTG TGACCTGGAG 
GCCATTGCAG TTACAGGTGT GGGCCCTCTG CACATGCTGC CCAGCACCTG TCTCCAGAAA 
GTGGACGAGC AACCAGAGGA TGCAGACAAC CAGCGTAATG TCACCCGGAT GGGCAGTCAG 
CCCTCAGATC CCAGCACCAC TGTGCATGTC CCAGTGACAC TGACAGGCCC TCCTGGGGAG 
ACCCCTGTAG TTCCCAGTGG TAACATGAAC CTGGAAGGCC AAGCAGAGGG CAAGAAGGAG 
GCAGAGGCGG ATGATGTGCT GAGAAGAGGC CCCAGGCCCA TCGTTCCCTA CAGCTCCATG 
TTTTGTCTCA GCCCCACCAA CCTGCTTCGT CGCTTCTGCC ATTACATTGT GACCATGCGG 
TACTTTGAGA TGGTAATTCT TGTGGTCATT GCCTTGAGCA GCATTGCCCT GGCTGCAGAG 
GATCCTGTGC GGACAGATTC ATTCAGGAAC AACGCTTTAA AGTACATGGA TTACATCTTT 
ACAGGAGTCT TCACCTTTGA AATGGTCATA AAGATGATAG ACTTGGGCTT GCTGCTGCAC 
CCTGGTGCCT ACTTCCGGGA CCTGTGGAAC ATTCTGGACT TCATTGTTGT CAGTGGAGCC 
CTGGTGGCAT TTGCGTTCTC AGGATCCAAA GGGAAAGACA TCAATACCAT CAAGTCTCTG 
AGAGTCCTGC GTGTCCTGAG GCCCCTCAAG ACCATCAAGC GGCTGCCTAA GCTCAAGGCT 
GTCTTTGACT GTGTGGTGAA CTCCCTGAAG AACGTCTTGA ACATCCTGAT TGTCTACATG 
CTCTTCATGT TCATATTTGC CGTCATTGCC GTCCAGCTCT TCAAAGGGAA GTTCTTTTAC 
TGTACTGATG AATCCAAGGA GCTGGAGAGG GACTGCCGGG GTCAGTATTT GGATTATGAG 
AAGGAAGAAG TAGAAGCCCA GCCAAGGCAG TGGAAGAAAT ATGACTTCCA CTATGACAAT 
GTTCTCTGGG CCTTGTTGAC GCTGTTCACA GTGTCCACGG GAGAGGGGTG GCCCATGGTG 
CTGAAACACT CTGTGGATGC CACCTATGAG GAACAGGGGC CCAGTCCCGG GTTCCGGATG 
GAGCTCTCCA TCTTCTACGT GGTCTACTTT GTGGTCTTCC CTTTTTTCTT TGTCAACATC 
TTTGTGGCCT TGATCATTAT CACCTTCCAG GAACAGGGAG ATAAGGTGAT GTCTGAATGC 
AGCTTAGAAA AGAATGAGAG GGCTTGCATT GATTTTGCCA TCAGTGCCAA GCCCCTGACA 
CGGTACATGC CTCAAAACAA ACAGTCGTTC CAGTATAAGA CATGGACATT CGTGGTCTCT 
CCACCCTTTG AGTACTTCAT CATGGCTATG ATAGCCCTCA ACACAGTGGT GCTGATGATG 
AAGTTCTATG ATGCACCTTA TGAGTACGAG CTGATGCTGA AATGCCTGAA CATTGTCTTC 
ACATCCATGT TCTCGATGGA GTGCATACTG AAGATCATCG CCTTTGGGGT ATTGAACTAC 
TTCAGAGATG CCTGGAACGT CTTTGACTTT GTCACGGTTT TGGGAAGTAT TACTGATATT 
TTAGTAACAG AGATAGCGAA CAACTTCATC AACCTAAGCT TCCTTCGCCT CTTCCGGGCG 
GCACGGCTGA TCAAGCTGCT TCGCCAGGGC TACACCATCC GCATCCTATT GTGGACCTTC 
GTCCAGTCCT TTAAGGCGCT GCCCTACGTG TGCCTCCTCA TTGCCATGCT GTTCTTCATC 
TACGCCATCA TCGGCATGCA GGTTTTTGGA AACATTGCCC TTGATGATGA CACCAGTATC 
AACCGACACA ACAACTTCCG GACATTTCTG CAAGCCTTAA TGCTATTGTT CAGGAGTGCC 
ACTGGGGAGG CCTGGCATGA GATCATGCTG TCTTGTCTGG GCAACCGGGC CTGTGACCCA 
CATGCCAACG CCAGTGAGTG CGGGAGCGAC TTTGCCTATT TTTATTTTGT CTCCTTCATC 
TTCCTCTGTT CCTTTCTGAT GTTGAACCTC TTTGTTGCTG TAATCATGGA CAATTTTGAG 
TACCTCACTC GGGACTCTTC CATCCTAGGG CCTCACCACT TAGACGAATT CATTCGAGTC 
TGGGCTGAAT ACGACCCAGC TGCGTGTGGG CGCATCAGTT ACAATGACAT GTTTGAGATG 
CTGAAACACA TGTCCCCACC TCTGGGGTTG GGGAAGAAAT GCCCGGCTCG AGTTGCATAC 
AAGCGCCTGG TTCGCATGAA CATGCCCATA TCCAATGAGG ACATGACGGT GCACTTTACG 
TCCACACTGA TGGCCCTCAT CCGGACAGCA CTGGAGATCA AGCTTGCCCC AGCGGGGACG 
AAGCAGCACC AGTGTGATGC TGAGCTGAGA AAAGAGATCT CTTCTGTGTG GGCTAATCTG 
CCCCAGAAGA CTCTGGACTT ACTGGTACCA CCCCACAAAC CTGACGAGAT GACAGTGGGG 
AAGGTCTATG CTGCTCTCAT GATATTTGAC TTCTACAAAC AGAACAAAAC CACCAGAGAT 
CAGACTCACC AAGCTCCCGG AGGCCTGTCC CAGATGGGTC CCGTTTCCCT GTTCCACCCT 
CTGAAGGCCA CCCTGGAACA GACACAGCCC GCTGTGCTTC GAGGAGCTCG GGTTTTCCTT 
CGGCAAAAGA GTGCAACTTC CCTCAGCAAT GGGGGTGCCA TACAAACCCA GGAAAGTGGC 
ATCAAGGAGT CGCTGTCCTG GGGCACGCAG AGGACCCAAG ATGCACTTTA TGAGGCCAGA 
GCACCTCTAG AACGTGGCCA TTCTGCAGAG ATCCCTGTGG GGCAGTCAGG AACACTGGCT 
GTGGATGTCC AGATGCAGAA CATGACACTG AGAGGACCAG ATGGGGAGCC CCAGCCTGGG 
CTGGAAAGCC AAGGCAGAGC TGCCTCTATG CCACGCCTAG CGGCAGAAAC ACAGCCGGCC 
CCTAATGCCA GCCCCATGAA GCGCTCCATC TCCACACTGG CTCCACGCCC ACATGGGACT 
CAGCTTTGCA GCACAGTTCT GGACCGGCCT CCTCCTAGCC AGGCATCACA TCACCACCAC 
CACCGCTGCC ACCGGCGCAG AGACAAGAAG CAAAGGTCCC TGGAAAAGGG GCCCAGCCTG 
TCTGTTGACC CAGAAGGTGC ACCAAGCACT GCTGCAGGAC CTGGTCTGCC CCATGGAGAA 
GGATCCACCG CCTGCCGGCG GGACCGTAAA CAGGAGCGAG GCCGGTCCCA GGAGCGGAGG 
CAGCCCTCAT CTTCCTCTTC AGAGAAGCAG CGCTTCTATT CCTGTGACCG CTTTGGGAGC 
CGGGAGCCCC CGCAACTGAT GCCCTCACTC AGTAGCCACC CCACATCGCC AACAGCGGCG 
CTAGAGCCAG CACCCCACCC ACAGGGCAGT GGTTCCGTTA ATGGGAGCCC CTTGATGTCA 
ACATCCGGTG CTAGCACCCC GGGCCGAGGT GGGCGGAGGC AGCTCCCCCA GACTCCTCTG 
ACCCCACGCC CCAGCATCAC CTACAAGACC GCCAATTCCT CGCCTGTCCA CTTTGCTGAG 
GGTCAGAGCG GCCTCCCAGC CTTCTCCCCT GGCCGTCTCA GCCGCGGCCT TTCTGAACAC 
AATGCCCTGC TCCAGAAAGA GCCCCTGAGC CAGCCTCTAG CTCCTGGCTC CCGAATTGGC 
TCTGACCCTT ACCTAGGGCA GCGTCTGGAC AGTGAGGCCT CCGCCCACAC CCTGCCTGAG 
GATACACTCA CCTTTGAAGA GGCAGTGGCC ACCAACTCTG GCCGCTCCTC CAGGACTTCC 
TATGTGTCCT CCCTCACTTC CCAATCCCAC CCTCTCCGCC GTGTACCCAA TGGCTATCAC 
TGCACTTTGG GACTCAGCAC TGGCGTCCGG GCGCGGCACA GCTACCACCA CCCCGATCAG 
GACCACTGGT GCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Cell-Specific RNA Binding Protein Rbfox2 Regulates Ca
eNeuro4(5)(2017 Sep-Oct)
Allen SE,Toro CP,Andrade A,L?pez-Soto EJ,Denome S,Lipscombe D

N-type Ca
Neurobiology of aging551-10(2017 Jul)
Silva FR,Miranda AS,Santos RPM,Olmo IG,Zamponi GW,Dobransky T,Cruz JS,Vieira LB,Ribeiro FM

High-throughput discovery of novel developmental phenotypes.
Nature537(7621)508-514(2016 09)
Dickinson ME,Flenniken AM,Ji X,Teboul L,Wong MD,White JK,Meehan TF,Weninger WJ,Westerberg H,Adissu H,Baker CN,Bower L,Brown JM,Caddle LB,Chiani F,Clary D,Cleak J,Daly MJ,Denegre JM,Doe B,Dolan ME,Edie SM,Fuchs H,Gailus-Durner V,Galli A,Gambadoro A,Gallegos J,Guo S,Horner NR,Hsu CW,Johnson SJ,Kalaga S,Keith LC,Lanoue L,Lawson TN,Lek M,Mark M,Marschall S,Mason J,McElwee ML,Newbigging S,Nutter LM,Peterson KA,Ramirez-Solis R,Rowland DJ,Ryder E,Samocha KE,Seavitt JR,Selloum M,Szoke-Kovacs Z,Tamura M,Trainor AG,Tudose I,Wakana S,Warren J,Wendling O,West DB,Wong L,Yoshiki A,,,,,,,,,MacArthur DG,Tocchini-Valentini GP,Gao X,Flicek P,Bradley A,Skarnes WC,Justice MJ,Parkinson HE,Moore M,Wells S,Braun RE,Svenson KL,de Angelis MH,Herault Y,Mohun T,Mallon AM,Henkelman RM,Brown SD,Adams DJ,Lloyd KC,McKerlie C,Beaudet AL,Bu?an M,Murray SA

Ablation of the N-type calcium channel ameliorates diabetic nephropathy with improved glycemic control and reduced blood pressure.
Scientific reports627192(2016 Jun)
Ohno S,Yokoi H,Mori K,Kasahara M,Kuwahara K,Fujikura J,Naito M,Kuwabara T,Imamaki H,Ishii A,Saleem MA,Numata T,Mori Y,Nakao K,Yanagita M,Mukoyama M

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Cacna1b cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

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Target ORF information:

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

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Target ORF information:

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ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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