Glb1 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Glb1
Entrez Gene ID	12091
Full Name	galactosidase, beta 1
Synonyms	AW125515,Bge,Bgl,Bgl-e,Bgl-s,Bgl-t,Bgs,Bgt,C130097A14Rik
General protein information	Preferred Names galactosidase, beta 1 Names beta-galactosidase acid beta-galactosidase lactase
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 9 Map Location: 9 F3\|9 64.4 cM
Summary	This gene encodes a preproprotein that is proteolytically cleaved to yield a signal peptide and a proproptein that is subsequently processed to generate the active mature peptide. The encoded protein is a lysosomal enzyme that catalyzes the hydrolysis of terminal beta-D-galactose residues in various substrates like lactose, ganglioside GM1 and other glycoproteins. Mutations in the human gene are associated with GM1-gangliosidosis and Morquio B syndrome. Disruption of the mouse gene mirrors the symptoms of human gangliosidosis. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2013].

mRNA and Protein(s)

mRNA	Protein	Name
NM_009752.2	NP_033882.1	beta-galactosidase preproprotein

Pathways from BioSystems

Homologies

Homo sapiens (human)	GLB1	NP_001073279.1
Oryza sativa (rice)	Os05g0539400	NP_001056172.1
Canis lupus familiaris (dog)	GLB1	NP_001032730.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP002056	XP_001689312.2
Pan troglodytes (chimpanzee)	GLB1	XP_003950098.1
	LOC709355	XP_001097877.1
Bos taurus (cattle)	GLB1	NP_001030215.1
Mus musculus (house mouse)	Glb1	NP_033882.1
Rattus norvegicus (Norway rat)	Glb1	NP_001101662.1
Danio rerio (zebrafish)	glb1	NP_001017547.1
Caenorhabditis elegans (roundworm)	T19B10.3	NP_505849.2
Caenorhabditis elegans (roundworm)	H22K11.2	NP_509140.2
Arabidopsis thaliana (thale cress)	BGAL17	NP_565051.1
Gallus gallus (chicken)	GLB1	NP_001265076.1
Xenopus tropicalis (tropical clawed frog)	glb1l	NP_001015958.1

Gene Ontology
Bibliography

Related articles in PubMed

Stem cell senescence drives age-attenuated induction of pituitary tumours in mouse models of paediatric craniopharyngioma.
Mario Gonzalez-Meljem J, Haston S, Carreno G, Apps JR, Pozzi S, Stache C, Kaushal G, Virasami A, Panousopoulos L, Neda Mousavy-Gharavy S, Guerrero A, Rashid M, Jani N, Goding CR, Jacques TS, Adams DJ, Gil J, Andoniadou CL, Pedro Martinez-Barbera J
Nature communications8(1)1819(2017 Nov)

p16(Ink4a) and senescence-associated ?-galactosidase can be induced in macrophages as part of a reversible response to physiological stimuli.
Hall BM, Balan V, Gleiberman AS, Strom E, Krasnov P, Virtuoso LP, Rydkina E, Vujcic S, Balan K, Gitlin II, Leonova KI, Consiglio CR, Gollnick SO, Chernova OB, Gudkov AV
Aging9(8)1867-1884(2017 Aug)

Spatiotemporal Analysis of a Glycolytic Activity Gradient Linked to Mouse Embryo Mesoderm Development.
Bulusu V, Prior N, Snaebjornsson MT, Kuehne A, Sonnen KF, Kress J, Stein F, Schultz C, Sauer U, Aulehla A
Developmental cell40(4)331-341.e4(2017 02)

Molecular cloning of mouse acid beta-galactosidase cDNA: sequence, expression of catalytic activity and comparison with the human enzyme.
Nanba E, Suzuki K
Biochemical and biophysical research communications173(1)141-8(1990 Nov)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

beta-gal is present in the pro-urine from where it is thought to stimulate TRPV5 activity.
Title: Urinary ?-galactosidase stimulates Ca2+ transport by stabilizing TRPV5 at the plasma membrane.

This study shows that moderate widespread expression of betagal in the CNS of GM1 gangliosidosis mice is sufficient to achieve significant biochemical impact with phenotypic amelioration and extension in lifespan
Title: Systemic AAV9 gene transfer in adult GM1 gangliosidosis mice reduces lysosomal storage in CNS and extends lifespan.

Senescence-associated beta-galactosidase is present in apical vacuoles of visceral endoderm in developing mouse embryos.
Title: Senescence-associated ?-galactosidase activity marks the visceral endoderm of mouse embryos but is not indicative of senescence.

We show here that mouse GLB1 has greater stability when compared to human GLB1, and that human GLB1 activity is temperature and protective-dependent on protein cathepsin A, while that of mouse GLB1 is not
Title: Murine ?-galactosidase stability is not dependent on temperature or protective protein/cathepsin A.

This protein was used as a biological marker of cell lineage, cell differentiation, and cell movement in the mouse cerebellum.
Title: Progressive restriction of cell fates in relation to neuroepithelial cell mingling in the mouse cerebellum.

The following Glb1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Glb1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu04770	NM_009752.2 Latest version!	Mus musculus galactosidase, beta 1 (Glb1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$384.30 $549.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Glb1 ( NM_009752.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_033882.1 Mus musculus galactosidase, beta 1 (Glb1), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OMu04770
Clone ID Related Accession (Same CDS sequence)	NM_009752.2
Accession Version	NM_009752.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1944bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-10
Organism	Mus musculus(house mouse)
Product	beta-galactosidase preproprotein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK171593.1, AC156499.2 and AK153218.1. On Dec 7, 2013 this sequence version replaced NM_009752.1. Transcript Variant: This variant (1) represents the longest transcript and encodes the functional protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK166473.1, AK171593.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: full length.

ATGCTCCGGG TCCCCCTGTG TACGCCGCTC CCGCTCCTGG CACTGCTGCA ACTGCTGGGC 
GCTGCGCACG GCATCTATAA TGTCACCCAG AGGACATTTA AGCTCGACTA CAGCCGGGAC 
CGCTTCCTCA AGGATGGACA GCCATTCCGA TACATCTCGG GAAGCATTCA TTACTTCCGG 
ATACCCCGCT TCTACTGGGA GGACCGGCTG CTGAAGATGA AGATGGCTGG GCTGAATGCT 
ATCCAGATGT ACGTGCCCTG GAACTTCCAT GAACCCCAAC CAGGACAATA TGAGTTTTCT 
GGGGACCGTG ATGTGGAGCA TTTCATCCAG CTGGCTCATG AGCTGGGACT CCTGGTGATC 
CTGAGGCCTG GGCCCTACAT CTGTGCAGAG TGGGACATGG GGGGCTTACC TGCTTGGCTA 
CTAGAGAAAC AATCTATCGT TCTCCGGTCT TCTGACCCAG ACTACCTTGT AGCTGTGGAT 
AAATGGCTGG CAGTCCTTCT GCCCAAGATG AAGCCCCTGC TCTACCAGAA CGGAGGACCG 
ATCATAACCG TGCAGGTTGA GAATGAGTAC GGGTCCTACT TTGCCTGCGA TTACGACTAC 
CTACGCTTCC TGGTGCACCG CTTCCGCTAC CATCTGGGTA ATGACGTCAT TCTCTTCACC 
ACCGACGGAG CAAGTGAAAA AATGCTGAAG TGTGGGACCC TGCAGGACCT GTACGCCACA 
GTGGATTTTG GAACAGGCAA CAATATCACA CAAGCTTTCC TGGTCCAGAG GAAGTTTGAA 
CCTAAAGGAC CTTTGATCAA TTCTGAGTTC TATACTGGCT GGCTAGACCA CTGGGGTAAA 
CCCCATTCCA CGGTGAAAAC TAAAACACTG GCTACCTCCC TCTATAACCT GCTTGCCCGT 
GGGGCCAACG TGAACTTGTA CATGTTTATA GGTGGGACCA ATTTTGCCTA TTGGAATGGT 
GCCAACACGC CCTATGAGCC ACAGCCCACC AGCTATGACT ACGACGCCCC ACTGAGCGAG 
GCTGGGGACC TCACTAAGAA GTATTTTGCT CTTCGAGAAG TCATTCAGAT GTTTAAAGAA 
GTCCCAGAAG GCCCTATCCC TCCGTCTACA CCCAAATTTG CATATGGAAA AGTTGCTCTG 
AGAAAGTTCA AGACAGTGGC TGAAGCTCTG GGTATCCTGT GTCCCAATGG GCCAGTGAAA 
AGCCTCTATC CCCTGACATT CACTCAGGTA AAACAGTATT TTGGGTATGT GCTGTACCGA 
ACAACGCTTC CTCAAGATTG CAGTAACCCG AAACCCATTT TCTCTTCACC CTTCAATGGT 
GTCCGTGATC GGGCTTACGT CTCTGTGGAC GGGGTCCCCC AAGGAATCCT TGATCGAAAC 
CTCATGACAG CTCTGAACAT ACGGGGGAAG GCTGGAGCCA CGCTGGACAT CCTGGTGGAG 
AACATGGGGC GTGTGAACTA TGGCAGATTC ATCAATGACT TCAAGGGTTT GATTTCCAAC 
ATGACTATCA ACTCCACTGT CCTCACCAAC TGGACGGTCT TCCCACTGAA CACTGAGGCC 
ATGGTACGCA ACCATCTCTG GGGCCGGGAG GCCAGTGATG AGGGTCACCT TGACGGACGG 
TCGACCTCCA ATTCTTCGGA CCTCATACTC CCCACCTTTT ACGTGGGCAA CTTCTCCATC 
CCCTCGGGCA TCCCAGACCT GCCACAGGAC ACCTTCATCC AGTTTCCTGG GTGGTCCAAG 
GGTCAAGTAT GGATCAATGG CTTTAACCTC GGCCGATACT GGCCCACAAT GGGCCCACAA 
AAGACCTTGT TCGTGCCAAG GAACATCCTG ACCACTTCAG CCCCAAACAA CATCACAGTG 
TTGGAGCTAG AGTTTGCACC CTGCAGCGAG GGGACCCCAG AGCTGTGTAC AGTAGAGTTT 
GTTGACACTC CGGTCATTTC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_033882.1
CDS	115..2058
Translation	MLRVPLCTPLPLLALLQLLGAAHGIYNVTQRTFKLDYSRDRFLKDGQPFRYISGSIHYFRIPRFYWEDRLLKMKMAGLNAIQMYVPWNFHEPQPGQYEFSGDRDVEHFIQLAHELGLLVILRPGPYICAEWDMGGLPAWLLEKQSIVLRSSDPDYLVAVDKWLAVLLPKMKPLLYQNGGPIITVQVENEYGSYFACDYDYLRFLVHRFRYHLGNDVILFTTDGASEKMLKCGTLQDLYATVDFGTGNNITQAFLVQRKFEPKGPLINSEFYTGWLDHWGKPHSTVKTKTLATSLYNLLARGANVNLYMFIGGTNFAYWNGANTPYEPQPTSYDYDAPLSEAGDLTKKYFALREVIQMFKEVPEGPIPPSTPKFAYGKVALRKFKTVAEALGILCPNGPVKSLYPLTFTQVKQYFGYVLYRTTLPQDCSNPKPIFSSPFNGVRDRAYVSVDGVPQGILDRNLMTALNIRGKAGATLDILVENMGRVNYGRFINDFKGLISNMTINSTVLTNWTVFPLNTEAMVRNHLWGREASDEGHLDGRSTSNSSDLILPTFYVGNFSIPSGIPDLPQDTFIQFPGWSKGQVWINGFNLGRYWPTMGPQKTLFVPRNILTTSAPNNITVLELEFAPCSEGTPELCTVEFVDTPVIS

Target ORF information:

RefSeq Version	NM_009752.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus galactosidase, beta 1 (Glb1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_009752.2

ORF Insert Sequence:

ATGCTCCGGG TCCCCCTGTG TACGCCGCTC CCGCTCCTGG CACTGCTGCA ACTGCTGGGC 
GCTGCGCACG GCATCTATAA TGTCACCCAG AGGACATTTA AGCTCGACTA CAGCCGGGAC 
CGCTTCCTCA AGGATGGACA GCCATTCCGA TACATCTCGG GAAGCATTCA TTACTTCCGG 
ATACCCCGCT TCTACTGGGA GGACCGGCTG CTGAAGATGA AGATGGCTGG GCTGAATGCT 
ATCCAGATGT ACGTGCCCTG GAACTTCCAT GAACCCCAAC CAGGACAATA TGAGTTTTCT 
GGGGACCGTG ATGTGGAGCA TTTCATCCAG CTGGCTCATG AGCTGGGACT CCTGGTGATC 
CTGAGGCCTG GGCCCTACAT CTGTGCAGAG TGGGACATGG GGGGCTTACC TGCTTGGCTA 
CTAGAGAAAC AATCTATCGT TCTCCGGTCT TCTGACCCAG ACTACCTTGT AGCTGTGGAT 
AAATGGCTGG CAGTCCTTCT GCCCAAGATG AAGCCCCTGC TCTACCAGAA CGGAGGACCG 
ATCATAACCG TGCAGGTTGA GAATGAGTAC GGGTCCTACT TTGCCTGCGA TTACGACTAC 
CTACGCTTCC TGGTGCACCG CTTCCGCTAC CATCTGGGTA ATGACGTCAT TCTCTTCACC 
ACCGACGGAG CAAGTGAAAA AATGCTGAAG TGTGGGACCC TGCAGGACCT GTACGCCACA 
GTGGATTTTG GAACAGGCAA CAATATCACA CAAGCTTTCC TGGTCCAGAG GAAGTTTGAA 
CCTAAAGGAC CTTTGATCAA TTCTGAGTTC TATACTGGCT GGCTAGACCA CTGGGGTAAA 
CCCCATTCCA CGGTGAAAAC TAAAACACTG GCTACCTCCC TCTATAACCT GCTTGCCCGT 
GGGGCCAACG TGAACTTGTA CATGTTTATA GGTGGGACCA ATTTTGCCTA TTGGAATGGT 
GCCAACACGC CCTATGAGCC ACAGCCCACC AGCTATGACT ACGACGCCCC ACTGAGCGAG 
GCTGGGGACC TCACTAAGAA GTATTTTGCT CTTCGAGAAG TCATTCAGAT GTTTAAAGAA 
GTCCCAGAAG GCCCTATCCC TCCGTCTACA CCCAAATTTG CATATGGAAA AGTTGCTCTG 
AGAAAGTTCA AGACAGTGGC TGAAGCTCTG GGTATCCTGT GTCCCAATGG GCCAGTGAAA 
AGCCTCTATC CCCTGACATT CACTCAGGTA AAACAGTATT TTGGGTATGT GCTGTACCGA 
ACAACGCTTC CTCAAGATTG CAGTAACCCG AAACCCATTT TCTCTTCACC CTTCAATGGT 
GTCCGTGATC GGGCTTACGT CTCTGTGGAC GGGGTCCCCC AAGGAATCCT TGATCGAAAC 
CTCATGACAG CTCTGAACAT ACGGGGGAAG GCTGGAGCCA CGCTGGACAT CCTGGTGGAG 
AACATGGGGC GTGTGAACTA TGGCAGATTC ATCAATGACT TCAAGGGTTT GATTTCCAAC 
ATGACTATCA ACTCCACTGT CCTCACCAAC TGGACGGTCT TCCCACTGAA CACTGAGGCC 
ATGGTACGCA ACCATCTCTG GGGCCGGGAG GCCAGTGATG AGGGTCACCT TGACGGACGG 
TCGACCTCCA ATTCTTCGGA CCTCATACTC CCCACCTTTT ACGTGGGCAA CTTCTCCATC 
CCCTCGGGCA TCCCAGACCT GCCACAGGAC ACCTTCATCC AGTTTCCTGG GTGGTCCAAG 
GGTCAAGTAT GGATCAATGG CTTTAACCTC GGCCGATACT GGCCCACAAT GGGCCCACAA 
AAGACCTTGT TCGTGCCAAG GAACATCCTG ACCACTTCAG CCCCAAACAA CATCACAGTG 
TTGGAGCTAG AGTTTGCACC CTGCAGCGAG GGGACCCCAG AGCTGTGTAC AGTAGAGTTT 
GTTGACACTC CGGTCATTTC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Stem cell senescence drives age-attenuated induction of pituitary tumours in mouse models of paediatric craniopharyngioma.
Nature communications8(1)1819(2017 Nov)
Mario Gonzalez-Meljem J,Haston S,Carreno G,Apps JR,Pozzi S,Stache C,Kaushal G,Virasami A,Panousopoulos L,Neda Mousavy-Gharavy S,Guerrero A,Rashid M,Jani N,Goding CR,Jacques TS,Adams DJ,Gil J,Andoniadou CL,Pedro Martinez-Barbera J

p16(Ink4a) and senescence-associated ?-galactosidase can be induced in macrophages as part of a reversible response to physiological stimuli.
Aging9(8)1867-1884(2017 Aug)
Hall BM,Balan V,Gleiberman AS,Strom E,Krasnov P,Virtuoso LP,Rydkina E,Vujcic S,Balan K,Gitlin II,Leonova KI,Consiglio CR,Gollnick SO,Chernova OB,Gudkov AV

Spatiotemporal Analysis of a Glycolytic Activity Gradient Linked to Mouse Embryo Mesoderm Development.
Developmental cell40(4)331-341.e4(2017 02)
Bulusu V,Prior N,Snaebjornsson MT,Kuehne A,Sonnen KF,Kress J,Stein F,Schultz C,Sauer U,Aulehla A

Molecular cloning of mouse acid beta-galactosidase cDNA: sequence, expression of catalytic activity and comparison with the human enzyme.
Biochemical and biophysical research communications173(1)141-8(1990 Nov)
Nanba E,Suzuki K

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Glb1 cDNA ORF clone, Mus musculus(house mouse)

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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