Arf5 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Arf5
Entrez Gene ID	11844
Full Name	ADP-ribosylation factor 5
General protein information	Preferred Names ADP-ribosylation factor 5 Names ADP-ribosylation factor 5
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 6 Map Location: 6\|6 A3.3

mRNA and Protein(s)

mRNA	Protein	Name
NM_007480.2	NP_031506.1	ADP-ribosylation factor 5
NM_007480.1	NP_031506.1	ADP-ribosylation factor 5

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	ARF5	XP_519351.3
Bos taurus (cattle)	ARF5	NP_001077140.1
Danio rerio (zebrafish)	arf5	NP_954969.1
Homo sapiens (human)	ARF5	NP_001653.1
Rattus norvegicus (Norway rat)	Arf5	NP_077063.1
Danio rerio (zebrafish)	zgc:77650	NP_956170.1
Mus musculus (house mouse)	Arf5	NP_031506.1
Caenorhabditis elegans (roundworm)	arf-3	NP_501336.1
Canis lupus familiaris (dog)	ARF5	XP_532438.3

Gene Ontology
Bibliography

Related articles in PubMed

Calcium-dependent activator protein for secretion 2 interacts with the class II ARF small GTPases and regulates dense-core vesicle trafficking.
Sadakata T, Sekine Y, Oka M, Itakura M, Takahashi M, Furuichi T
The FEBS journal279(3)384-94(2012 Feb)

Interaction of calcium-dependent activator protein for secretion 1 (CAPS1) with the class II ADP-ribosylation factor small GTPases is required for dense-core vesicle trafficking in the trans-Golgi network.
Sadakata T, Shinoda Y, Sekine Y, Saruta C, Itakura M, Takahashi M, Furuichi T
The Journal of biological chemistry285(49)38710-9(2010 Dec)

Identification of candidate alkylator-induced cancer susceptibility genes by whole genome scanning in mice.
Fenske TS, McMahon C, Edwin D, Jarvis JC, Cheverud JM, Minn M, Mathews V, Bogue MA, Province MA, McLeod HL, Graubert TA
Cancer research66(10)5029-38(2006 May)

Structure and intracellular localization of mouse ADP-ribosylation factors type 1 to type 6 (ARF1-ARF6).
Hosaka M, Toda K, Takatsu H, Torii S, Murakami K, Nakayama K
Journal of biochemistry120(4)813-9(1996 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

somal CAPS2 is associated with the Golgi membrane, and mediates binding and recruitment of the GDP-bound form of ARF4 and ARF5 (members of the membrane-trafficking small GTPase family) to the Golgi membrane
Title: Calcium-dependent activator protein for secretion 2 interacts with the class II ARF small GTPases and regulates dense-core vesicle trafficking.

a functional role for CAPS1 in regulating DCV trafficking in the trans-Golgi network; this activity occurs via protein-protein interaction with ARF4/ARF5 in a GDP-dependent manner.
Title: Interaction of calcium-dependent activator protein for secretion 1 (CAPS1) with the class II ADP-ribosylation factor small GTPases is required for dense-core vesicle trafficking in the trans-Golgi network.

The following Arf5 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Arf5 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu00251	NM_007480.2 Latest version!	Mus musculus ADP-ribosylation factor 5 (Arf5), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$69.30 $99.00
View Old Accession Versions >>
OMu00251	NM_007480.1	Mus musculus ADP-ribosylation factor 5 (Arf5), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$69.30 $99.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Arf5 ( NM_007480.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_031506.1 Mus musculus ADP-ribosylation factor 5 (Arf5), mRNA.

Price & Availability↑

CloneID	OMu00251
Clone ID Related Accession (Same CDS sequence)	NM_007480.2 , NM_007480.1
Accession Version	NM_007480.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 543bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-11
Organism	Mus musculus(house mouse)
Product	ADP-ribosylation factor 5
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from CX224671.1, BF469348.1 and BF456113.1. On Feb 13, 2018 this sequence version replaced NM_007480.1. ##Evidence-Data-START## Transcript exon combination :: SRR1660817.409179.1, D87902.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END##

ATGGGCCTCA CGGTGTCCGC GCTCTTTTCG CGGATCTTCG GGAAGAAGCA GATGCGGATC 
CTTATGGTTG GCTTGGATGC GGCTGGCAAG ACTACCATCC TGTACAAACT GAAGTTGGGG 
GAGATTGTCA CCACCATCCC CACTATAGGC TTCAATGTGG AAACAGTGGA ATATAAGAAC 
ATCTGTTTCA CAGTGTGGGA TGTTGGAGGC CAGGATAAGA TTCGGCCTCT GTGGCGGCAC 
TACTTCCAGA ACACTCAGGG CCTCATCTTT GTGGTAGACA GCAACGACCG GGAGCGGGTC 
CAGGAGTCTG CTGATGAACT CCAGAAGATG CTGCAGGAGG ATGAGCTCCG GGATGCGGTG 
CTGCTGGTGT TTGCCAACAA GCAGGACATG CCCAATGCCA TGCCCGTGAG CGAGCTGACT 
GACAAGCTGG GCCTTCAGCA CCTGCGTAGC CGCACGTGGT ACGTCCAGGC CACCTGTGCC 
ACCCAAGGCA CAGGCCTGTA TGATGGGCTG GACTGGCTGT CCCACGAGCT GTCAAAGCGC 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_031506.1
CDS	86..628
Translation	MGLTVSALFSRIFGKKQMRILMVGLDAAGKTTILYKLKLGEIVTTIPTIGFNVETVEYKNICFTVWDVGGQDKIRPLWRHYFQNTQGLIFVVDSNDRERVQESADELQKMLQEDELRDAVLLVFANKQDMPNAMPVSELTDKLGLQHLRSRTWYVQATCATQGTGLYDGLDWLSHELSKR

Target ORF information:

RefSeq Version	NM_007480.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus ADP-ribosylation factor 5 (Arf5), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_007480.2

ORF Insert Sequence:

ATGGGCCTCA CGGTGTCCGC GCTCTTTTCG CGGATCTTCG GGAAGAAGCA GATGCGGATC 
CTTATGGTTG GCTTGGATGC GGCTGGCAAG ACTACCATCC TGTACAAACT GAAGTTGGGG 
GAGATTGTCA CCACCATCCC CACTATAGGC TTCAATGTGG AAACAGTGGA ATATAAGAAC 
ATCTGTTTCA CAGTGTGGGA TGTTGGAGGC CAGGATAAGA TTCGGCCTCT GTGGCGGCAC 
TACTTCCAGA ACACTCAGGG CCTCATCTTT GTGGTAGACA GCAACGACCG GGAGCGGGTC 
CAGGAGTCTG CTGATGAACT CCAGAAGATG CTGCAGGAGG ATGAGCTCCG GGATGCGGTG 
CTGCTGGTGT TTGCCAACAA GCAGGACATG CCCAATGCCA TGCCCGTGAG CGAGCTGACT 
GACAAGCTGG GCCTTCAGCA CCTGCGTAGC CGCACGTGGT ACGTCCAGGC CACCTGTGCC 
ACCCAAGGCA CAGGCCTGTA TGATGGGCTG GACTGGCTGT CCCACGAGCT GTCAAAGCGC 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Arf5 ( NM_007480.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_031506.1 Mus musculus ADP-ribosylation factor 5 (Arf5), mRNA.

Price & Availability↑

CloneID	OMu00251
Clone ID Related Accession (Same CDS sequence)	NM_007480.2 , NM_007480.1
Accession Version	NM_007480.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 543bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-28
Organism	Mus musculus(house mouse)
Product	ADP-ribosylation factor 5
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from D87902.1. On Dec 7, 2005 this sequence version replaced XM_913125.1. ##Evidence-Data-START## Transcript exon combination :: D87902.1, SRR1660817.409179.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END##

ATGGGCCTCA CGGTGTCCGC GCTCTTTTCG CGGATCTTCG GGAAGAAGCA GATGCGGATC 
CTTATGGTTG GCTTGGATGC GGCTGGCAAG ACTACCATCC TGTACAAACT GAAGTTGGGG 
GAGATTGTCA CCACCATCCC CACTATAGGC TTCAATGTGG AAACAGTGGA ATATAAGAAC 
ATCTGTTTCA CAGTGTGGGA TGTTGGAGGC CAGGATAAGA TTCGGCCTCT GTGGCGGCAC 
TACTTCCAGA ACACTCAGGG CCTCATCTTT GTGGTAGACA GCAACGACCG GGAGCGGGTC 
CAGGAGTCTG CTGATGAACT CCAGAAGATG CTGCAGGAGG ATGAGCTCCG GGATGCGGTG 
CTGCTGGTGT TTGCCAACAA GCAGGACATG CCCAATGCCA TGCCCGTGAG CGAGCTGACT 
GACAAGCTGG GCCTTCAGCA CCTGCGTAGC CGCACGTGGT ACGTCCAGGC CACCTGTGCC 
ACCCAAGGCA CAGGCCTGTA TGATGGGCTG GACTGGCTGT CCCACGAGCT GTCAAAGCGC 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_031506.1
CDS	50..592
Translation	MGLTVSALFSRIFGKKQMRILMVGLDAAGKTTILYKLKLGEIVTTIPTIGFNVETVEYKNICFTVWDVGGQDKIRPLWRHYFQNTQGLIFVVDSNDRERVQESADELQKMLQEDELRDAVLLVFANKQDMPNAMPVSELTDKLGLQHLRSRTWYVQATCATQGTGLYDGLDWLSHELSKR

Target ORF information:

RefSeq Version	NM_007480.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus ADP-ribosylation factor 5 (Arf5), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_007480.1

ORF Insert Sequence:

ATGGGCCTCA CGGTGTCCGC GCTCTTTTCG CGGATCTTCG GGAAGAAGCA GATGCGGATC 
CTTATGGTTG GCTTGGATGC GGCTGGCAAG ACTACCATCC TGTACAAACT GAAGTTGGGG 
GAGATTGTCA CCACCATCCC CACTATAGGC TTCAATGTGG AAACAGTGGA ATATAAGAAC 
ATCTGTTTCA CAGTGTGGGA TGTTGGAGGC CAGGATAAGA TTCGGCCTCT GTGGCGGCAC 
TACTTCCAGA ACACTCAGGG CCTCATCTTT GTGGTAGACA GCAACGACCG GGAGCGGGTC 
CAGGAGTCTG CTGATGAACT CCAGAAGATG CTGCAGGAGG ATGAGCTCCG GGATGCGGTG 
CTGCTGGTGT TTGCCAACAA GCAGGACATG CCCAATGCCA TGCCCGTGAG CGAGCTGACT 
GACAAGCTGG GCCTTCAGCA CCTGCGTAGC CGCACGTGGT ACGTCCAGGC CACCTGTGCC 
ACCCAAGGCA CAGGCCTGTA TGATGGGCTG GACTGGCTGT CCCACGAGCT GTCAAAGCGC 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Calcium-dependent activator protein for secretion 2 interacts with the class II ARF small GTPases and regulates dense-core vesicle trafficking.
The FEBS journal279(3)384-94(2012 Feb)
Sadakata T,Sekine Y,Oka M,Itakura M,Takahashi M,Furuichi T

Interaction of calcium-dependent activator protein for secretion 1 (CAPS1) with the class II ADP-ribosylation factor small GTPases is required for dense-core vesicle trafficking in the trans-Golgi network.
The Journal of biological chemistry285(49)38710-9(2010 Dec)
Sadakata T,Shinoda Y,Sekine Y,Saruta C,Itakura M,Takahashi M,Furuichi T

Identification of candidate alkylator-induced cancer susceptibility genes by whole genome scanning in mice.
Cancer research66(10)5029-38(2006 May)
Fenske TS,McMahon C,Edwin D,Jarvis JC,Cheverud JM,Minn M,Mathews V,Bogue MA,Province MA,McLeod HL,Graubert TA

Structure and intracellular localization of mouse ADP-ribosylation factors type 1 to type 6 (ARF1-ARF6).
Journal of biochemistry120(4)813-9(1996 Oct)
Hosaka M,Toda K,Takatsu H,Torii S,Murakami K,Nakayama K

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Arf5 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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