Ap2a1 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Ap2a1
Entrez Gene ID	11771
Full Name	adaptor-related protein complex 2, alpha 1 subunit
Synonyms	Adtaa
General protein information	Preferred Names adaptor-related protein complex 2, alpha 1 subunit Names AP-2 complex subunit alpha-1 100 kDa coated vesicle protein A adapter-related protein complex 2 alpha-1 subunit adapter-related protein complex 2 subunit alpha-1 adaptor protein complex AP-2 subunit alpha-1 adaptor protein complex AP-2, alpha 1 subunit adaptor-related protein complex 2 subunit alpha-1 adaptor-related protein complex AP-2, alpha 1 subunit alpha-adaptin A alpha1-adaptin clathrin assembly protein complex 2 alpha-A large chain plasma membrane adaptor HA2/AP2 adaptin alpha A subunit
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 7 Map Location: 7\|7 B3

mRNA and Protein(s)

mRNA	Protein	Name
XM_006540570.1	XP_006540633.1	AP-2 complex subunit alpha-1 isoform X2
NM_007458.2	NP_031484.1	AP-2 complex subunit alpha-1 isoform a
XM_006540569.4	XP_006540632.1	AP-2 complex subunit alpha-1 isoform X1
XM_006540569.3	XP_006540632.1	AP-2 complex subunit alpha-1 isoform X1
NM_001077264.1	NP_001070732.1	AP-2 complex subunit alpha-1 isoform b

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	AP2A1	XP_003953618.1
Xenopus tropicalis (tropical clawed frog)	ap2a1	NP_001090846.1
Homo sapiens (human)	AP2A1	NP_570603.2
Mus musculus (house mouse)	Ap2a1	NP_031484.1
Canis lupus familiaris (dog)	AP2A1	XP_003638863.1
Rattus norvegicus (Norway rat)	Ap2a1	NP_001100981.1
Danio rerio (zebrafish)	ap2a1	XP_005164119.1
	AP2A1	XP_001115452.1
Bos taurus (cattle)	AP2A1	NP_001178369.1

Gene Ontology
Bibliography

Related articles in PubMed

APache Is an AP2-Interacting Protein Involved in Synaptic Vesicle Trafficking and Neuronal Development.
Piccini A, Castroflorio E, Valente P, Guarnieri FC, Aprile D, Michetti C, Bramini M, Giansante G, Pinto B, Savardi A, Cesca F, Bachi A, Cattaneo A, Wren JD, Fassio A, Valtorta F, Benfenati F, Gioved? S
Cell reports21(12)3596-3611(2017 Dec)

Surfactant Protein A Enhances Constitutive Immune Functions of Clathrin Heavy Chain and Clathrin Adaptor Protein 2.
Moulakakis C, Steinh?user C, Biedziak D, Freundt K, Reiling N, Stamme C
American journal of respiratory cell and molecular biology55(1)92-104(2016 Jul)

SPECC1L deficiency results in increased adherens junction stability and reduced cranial neural crest cell delamination.
Wilson NR, Olm-Shipman AJ, Acevedo DS, Palaniyandi K, Hall EG, Kosa E, Stumpff KM, Smith GJ, Pitstick L, Liao EC, Bjork BC, Czirok A, Saadi I
Scientific reports617735(2016 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

SP-A transiently enhances the basal protein expression of CHC and alpha-adaptin.
Title: Surfactant Protein A Enhances Constitutive Immune Functions of Clathrin Heavy Chain and Clathrin Adaptor Protein 2.

AP2 protein expression was regulated in a vitamin A-deficiennt mouse developing eye.
Title: Regulation of AP-2 and apoptosis in developing eye in a vitamin A-deficiency model.

The following Ap2a1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Ap2a1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu38641	XM_006540570.1 Latest version!	Mus musculus adaptor-related protein complex 2, alpha 1 subunit (Ap2a1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$307.30 $439.00
OMu20722	NM_001077264.1 Latest version!	Mus musculus adaptor-related protein complex 2, alpha 1 subunit (Ap2a1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$566.30 $809.00
OMu20235	NM_007458.2 Latest version!	Mus musculus adaptor-related protein complex 2, alpha 1 subunit (Ap2a1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$475.30 $679.00
OMu38640	XM_006540569.4 Latest version!	Mus musculus adaptor-related protein complex 2, alpha 1 subunit (Ap2a1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
View Old Accession Versions >>
OMu38640	XM_006540569.3	Mus musculus adaptor-related protein complex 2, alpha 1 subunit (Ap2a1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$475.30 $679.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Ap2a1 ( XM_006540569.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006540632.1 Mus musculus adaptor-related protein complex 2, alpha 1 subunit (Ap2a1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu38640
Clone ID Related Accession (Same CDS sequence)	XM_006540569.4 , XM_006540569.3
Accession Version	XM_006540569.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2706bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	AP-2 complex subunit alpha-1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000073.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006540569.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGCCG TGAACCTGCT AAGCTCTAAC AAGTACACGG AGAAGCAGAT AGGGTACCTG 
TTCATCTCAG TCCTGGTGAA CTCGAACTCA GAACTGATCC GGCTCATCAA CAACGCTATC 
AAGAATGACC TGGCCAGTCG CAACCCCACC TTCATGTGCC TGGCCTTGCA CTGTATCGCT 
AACGTGGGCA GCCGTGAGAT GGGCGAGGCT TTTGCTGCAG ACATTCCCCG AATCCTGGTG 
GCTGGGGACA GCATGGACAG TGTGAAGCAG AGTGCCGCCC TATGCCTACT GCGACTCTAC 
AAGGCCTCGC CCGACTTGGT GCCCATGGGC GAGTGGACGG CACGTGTAGT GCACTTGCTC 
AATGATCAGC ACATGGGAGT GGTCACAGCT GCTGTCAGCC TCATCACCTG TCTCTGCAAG 
AAGAATCCGG ATGACTTCAA GACCTGTATC TCCCTGGCTG TGTCTCGCTT AAGCCGGATC 
GTCTCCTCAG CCTCCACTGA CCTCCAGGAC TACACTTACT ACTTCGTTCC TGCACCCTGG 
CTCTCTGTGA AGCTACTGCG GCTGCTCCAG TGTTACCCAC CACCAGAGGA TGCAGCCGTG 
AAAGGGCGGT TAGTGGAGTG TCTGGAGACT GTGCTCAACA AGGCCCAGGA GCCTCCCAAG 
TCCAAGAAGG TGCAGCACTC CAACGCCAAG AACGCTATCC TCTTTGAGAC CATTAGCCTC 
ATCATCCACT ATGACAGTGA GCCCAACCTC CTGGTCCGCG CCTGCAACCA GCTGGGCCAG 
TTCCTGCAGC ACCGGGAGAC TAACCTGCGC TACCTGGCCC TGGAGAGCAT GTGCACGCTG 
GCCAGCTCCG AGTTCTCCCA CGAGGCCGTC AAGACCCACA TTGATACAGT TATTAATGCC 
CTCAAGACGG AGCGGGACGT CAGTGTGAGG CAGCGGGCGG CTGATCTCCT GTATGCCATG 
TGTGACCGGA GCAATGCCAA GCAGATTGTG TCAGAGATGC TGCGGTACCT GGAGACTGCT 
GACTATGCCA TCCGAGAGGA GATCGTGCTG AAGGTGGCCA TCCTGGCTGA GAAGTATGCA 
GTGGACTACA GCTGGTACGT GGACACCATC CTCAACCTCA TCCGCATCGC GGGCGACTAT 
GTGAGCGAGG AGGTGTGGTA CCGCGTGCTG CAGATCGTCA CCAACCGTGA TGACGTCCAG 
GGTTATGCTG CCAAGACAGT GTTTGAGGCC CTCCAGGCCC CAGCCTGTCA TGAGAACATG 
GTGAAGGTCG GTGGCTACAT CCTTGGGGAG TTTGGGAACT TGATTGCTGG GGACCCACGC 
TCCAGCCCAC CAGTGCAGTT CTCGCTGCTG CACTCCAAGT TCCACCTGTG CAGCGTGGCC 
ACCCGCGCTC TGTTGCTGTC CACCTACATC AAGTTCATCA ACCTCTTCCC TGAGACCAAG 
GCCACCATCC AAGGGGTTCT GCGTGCCGGC TCCCAGCTGC GAAATGCCGA CGTGGAGCTA 
CAGCAGCGGG CCGTGGAGTA CCTCACCCTC AGCTCCGTAG CCAGCACCGA TGTTCTGGCT 
ACGGTGCTAG AAGAAATGCC CCCATTTCCC GAGCGGGAGT CGTCCATCTT GGCCAAGCTG 
AAGCGCAAGA AGGGCCCTGG GGCAGCCAGT GCCCTAGATG ACAGCCGCAG GGACACCAGC 
AGCAATGACA TCAATGGGGG TGTGGAGCCC ACCCCCAGCA CTGTGTCGAC CCCCTCACCC 
TCCGCGGACC TCTTAGGGCT GCGGGCAGCC CCTCCCCCTG CTGCACCCCC GGCTCCCGTA 
GGCGGGAACC TCCTGGTGGA TGTCTTCTCT GACGGCCCCA CTGCACAGCC CAGCCTGGGG 
CCCACTCCTG AGGAGGCCTT CCTCAGCGAG CTGGAGCCCC CTGCCCCTGA GAGCCCCATG 
GCTTTGTTGG CTGACCCAGC TCCAGCTGCT GACCCAGGTC CTGAGGACAT AGGCCCTCCC 
ATTCCAGAAG CAGATGAACT GTTGAATAAG TTCGTGTGTA AGAATAGTGG GGTCTTGTTT 
GAGAACCAGC TGCTGCAGAT TGGAGTCAAG TCTGAGTTCC GGCAGAACCT GGGCCGCATG 
TATCTCTTCT ATGGCAACAA GACTTCTGTG CAGTTCCAGA ACTTCTTGCC CACCGTGGTC 
CATCCTGGGG ACCTCCAGAC TCAGCTGGCG GTGCAGACCA AGCGTGTGGC GGCACAAGTG 
GACGGTGGCG CACAGGTGCA GCAAGTACTC AACATTGAGT GTCTGCGAGA CTTCCTGACG 
CCGCCACTGT TGTCGGTGCG CTTCCGGTAC GGTGGCACCG CCCAGTCCCT CACTCTGAAG 
CTCCCAGTGA CCATCAACAA ATTCTTCCAG CCCACAGAGA TGGCGGCCCA AGACTTTTTC 
CAGCGCTGGA AGCAGCTGAG CCTCCCCCTG CAGGAGGCAC AGAAAATCTT CAAAGCCAAC 
CACCCGATGG ATGCTGAAGT TACTAAGGCC AAGCTTCTGG GGTTTGGCTC TGCTCTTCTG 
GACAATGTGG ATCCCAACCC TGAGAACTTT GTGGGTGCTG GAATCATCCA GACGAAGGCC 
CTGCAGGTGG GGTGTCTGCT TCGGCTGGAG CCCAATGCCC AGGCCCAAAT GTACCGTCTA 
ACCCTGCGCA CCAGCAAAGA GCCTGTCTCC CGTCACTTGT GTGAGCTGCT GGCCCAGCAG 
TTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006540632.1
CDS	473..3178
Translation	MEAVNLLSSNKYTEKQIGYLFISVLVNSNSELIRLINNAIKNDLASRNPTFMCLALHCIANVGSREMGEAFAADIPRILVAGDSMDSVKQSAALCLLRLYKASPDLVPMGEWTARVVHLLNDQHMGVVTAAVSLITCLCKKNPDDFKTCISLAVSRLSRIVSSASTDLQDYTYYFVPAPWLSVKLLRLLQCYPPPEDAAVKGRLVECLETVLNKAQEPPKSKKVQHSNAKNAILFETISLIIHYDSEPNLLVRACNQLGQFLQHRETNLRYLALESMCTLASSEFSHEAVKTHIDTVINALKTERDVSVRQRAADLLYAMCDRSNAKQIVSEMLRYLETADYAIREEIVLKVAILAEKYAVDYSWYVDTILNLIRIAGDYVSEEVWYRVLQIVTNRDDVQGYAAKTVFEALQAPACHENMVKVGGYILGEFGNLIAGDPRSSPPVQFSLLHSKFHLCSVATRALLLSTYIKFINLFPETKATIQGVLRAGSQLRNADVELQQRAVEYLTLSSVASTDVLATVLEEMPPFPERESSILAKLKRKKGPGAASALDDSRRDTSSNDINGGVEPTPSTVSTPSPSADLLGLRAAPPPAAPPAPVGGNLLVDVFSDGPTAQPSLGPTPEEAFLSELEPPAPESPMALLADPAPAADPGPEDIGPPIPEADELLNKFVCKNSGVLFENQLLQIGVKSEFRQNLGRMYLFYGNKTSVQFQNFLPTVVHPGDLQTQLAVQTKRVAAQVDGGAQVQQVLNIECLRDFLTPPLLSVRFRYGGTAQSLTLKLPVTINKFFQPTEMAAQDFFQRWKQLSLPLQEAQKIFKANHPMDAEVTKAKLLGFGSALLDNVDPNPENFVGAGIIQTKALQVGCLLRLEPNAQAQMYRLTLRTSKEPVSRHLCELLAQQF

Target ORF information:

RefSeq Version	XM_006540569.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus adaptor-related protein complex 2, alpha 1 subunit (Ap2a1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006540569.3

ORF Insert Sequence:

ATGGAGGCCG TGAACCTGCT AAGCTCTAAC AAGTACACGG AGAAGCAGAT AGGGTACCTG 
TTCATCTCAG TCCTGGTGAA CTCGAACTCA GAACTGATCC GGCTCATCAA CAACGCTATC 
AAGAATGACC TGGCCAGTCG CAACCCCACC TTCATGTGCC TGGCCTTGCA CTGTATCGCT 
AACGTGGGCA GCCGTGAGAT GGGCGAGGCT TTTGCTGCAG ACATTCCCCG AATCCTGGTG 
GCTGGGGACA GCATGGACAG TGTGAAGCAG AGTGCCGCCC TATGCCTACT GCGACTCTAC 
AAGGCCTCGC CCGACTTGGT GCCCATGGGC GAGTGGACGG CACGTGTAGT GCACTTGCTC 
AATGATCAGC ACATGGGAGT GGTCACAGCT GCTGTCAGCC TCATCACCTG TCTCTGCAAG 
AAGAATCCGG ATGACTTCAA GACCTGTATC TCCCTGGCTG TGTCTCGCTT AAGCCGGATC 
GTCTCCTCAG CCTCCACTGA CCTCCAGGAC TACACTTACT ACTTCGTTCC TGCACCCTGG 
CTCTCTGTGA AGCTACTGCG GCTGCTCCAG TGTTACCCAC CACCAGAGGA TGCAGCCGTG 
AAAGGGCGGT TAGTGGAGTG TCTGGAGACT GTGCTCAACA AGGCCCAGGA GCCTCCCAAG 
TCCAAGAAGG TGCAGCACTC CAACGCCAAG AACGCTATCC TCTTTGAGAC CATTAGCCTC 
ATCATCCACT ATGACAGTGA GCCCAACCTC CTGGTCCGCG CCTGCAACCA GCTGGGCCAG 
TTCCTGCAGC ACCGGGAGAC TAACCTGCGC TACCTGGCCC TGGAGAGCAT GTGCACGCTG 
GCCAGCTCCG AGTTCTCCCA CGAGGCCGTC AAGACCCACA TTGATACAGT TATTAATGCC 
CTCAAGACGG AGCGGGACGT CAGTGTGAGG CAGCGGGCGG CTGATCTCCT GTATGCCATG 
TGTGACCGGA GCAATGCCAA GCAGATTGTG TCAGAGATGC TGCGGTACCT GGAGACTGCT 
GACTATGCCA TCCGAGAGGA GATCGTGCTG AAGGTGGCCA TCCTGGCTGA GAAGTATGCA 
GTGGACTACA GCTGGTACGT GGACACCATC CTCAACCTCA TCCGCATCGC GGGCGACTAT 
GTGAGCGAGG AGGTGTGGTA CCGCGTGCTG CAGATCGTCA CCAACCGTGA TGACGTCCAG 
GGTTATGCTG CCAAGACAGT GTTTGAGGCC CTCCAGGCCC CAGCCTGTCA TGAGAACATG 
GTGAAGGTCG GTGGCTACAT CCTTGGGGAG TTTGGGAACT TGATTGCTGG GGACCCACGC 
TCCAGCCCAC CAGTGCAGTT CTCGCTGCTG CACTCCAAGT TCCACCTGTG CAGCGTGGCC 
ACCCGCGCTC TGTTGCTGTC CACCTACATC AAGTTCATCA ACCTCTTCCC TGAGACCAAG 
GCCACCATCC AAGGGGTTCT GCGTGCCGGC TCCCAGCTGC GAAATGCCGA CGTGGAGCTA 
CAGCAGCGGG CCGTGGAGTA CCTCACCCTC AGCTCCGTAG CCAGCACCGA TGTTCTGGCT 
ACGGTGCTAG AAGAAATGCC CCCATTTCCC GAGCGGGAGT CGTCCATCTT GGCCAAGCTG 
AAGCGCAAGA AGGGCCCTGG GGCAGCCAGT GCCCTAGATG ACAGCCGCAG GGACACCAGC 
AGCAATGACA TCAATGGGGG TGTGGAGCCC ACCCCCAGCA CTGTGTCGAC CCCCTCACCC 
TCCGCGGACC TCTTAGGGCT GCGGGCAGCC CCTCCCCCTG CTGCACCCCC GGCTCCCGTA 
GGCGGGAACC TCCTGGTGGA TGTCTTCTCT GACGGCCCCA CTGCACAGCC CAGCCTGGGG 
CCCACTCCTG AGGAGGCCTT CCTCAGCGAG CTGGAGCCCC CTGCCCCTGA GAGCCCCATG 
GCTTTGTTGG CTGACCCAGC TCCAGCTGCT GACCCAGGTC CTGAGGACAT AGGCCCTCCC 
ATTCCAGAAG CAGATGAACT GTTGAATAAG TTCGTGTGTA AGAATAGTGG GGTCTTGTTT 
GAGAACCAGC TGCTGCAGAT TGGAGTCAAG TCTGAGTTCC GGCAGAACCT GGGCCGCATG 
TATCTCTTCT ATGGCAACAA GACTTCTGTG CAGTTCCAGA ACTTCTTGCC CACCGTGGTC 
CATCCTGGGG ACCTCCAGAC TCAGCTGGCG GTGCAGACCA AGCGTGTGGC GGCACAAGTG 
GACGGTGGCG CACAGGTGCA GCAAGTACTC AACATTGAGT GTCTGCGAGA CTTCCTGACG 
CCGCCACTGT TGTCGGTGCG CTTCCGGTAC GGTGGCACCG CCCAGTCCCT CACTCTGAAG 
CTCCCAGTGA CCATCAACAA ATTCTTCCAG CCCACAGAGA TGGCGGCCCA AGACTTTTTC 
CAGCGCTGGA AGCAGCTGAG CCTCCCCCTG CAGGAGGCAC AGAAAATCTT CAAAGCCAAC 
CACCCGATGG ATGCTGAAGT TACTAAGGCC AAGCTTCTGG GGTTTGGCTC TGCTCTTCTG 
GACAATGTGG ATCCCAACCC TGAGAACTTT GTGGGTGCTG GAATCATCCA GACGAAGGCC 
CTGCAGGTGG GGTGTCTGCT TCGGCTGGAG CCCAATGCCC AGGCCCAAAT GTACCGTCTA 
ACCCTGCGCA CCAGCAAAGA GCCTGTCTCC CGTCACTTGT GTGAGCTGCT GGCCCAGCAG 
TTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ap2a1 ( XM_006540570.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006540633.1 Mus musculus adaptor-related protein complex 2, alpha 1 subunit (Ap2a1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OMu38641
Clone ID Related Accession (Same CDS sequence)	XM_006540570.1
Accession Version	XM_006540570.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1878bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	AP-2 complex subunit alpha-1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000073.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGCACGC TGGCCAGCTC CGAGTTCTCC CACGAGGCCG TCAAGACCCA CATTGATACA 
GTTATTAATG CCCTCAAGAC GGAGCGGGAC GTCAGTGTGA GGCAGCGGGC GGCTGATCTC 
CTGTATGCCA TGTGTGACCG GAGCAATGCC AAGCAGATTG TGTCAGAGAT GCTGCGGTAC 
CTGGAGACTG CTGACTATGC CATCCGAGAG GAGATCGTGC TGAAGGTGGC CATCCTGGCT 
GAGAAGTATG CAGTGGACTA CAGCTGGTAC GTGGACACCA TCCTCAACCT CATCCGCATC 
GCGGGCGACT ATGTGAGCGA GGAGGTGTGG TACCGCGTGC TGCAGATCGT CACCAACCGT 
GATGACGTCC AGGGTTATGC TGCCAAGACA GTGTTTGAGG CCCTCCAGGC CCCAGCCTGT 
CATGAGAACA TGGTGAAGGT CGGTGGCTAC ATCCTTGGGG AGTTTGGGAA CTTGATTGCT 
GGGGACCCAC GCTCCAGCCC ACCAGTGCAG TTCTCGCTGC TGCACTCCAA GTTCCACCTG 
TGCAGCGTGG CCACCCGCGC TCTGTTGCTG TCCACCTACA TCAAGTTCAT CAACCTCTTC 
CCTGAGACCA AGGCCACCAT CCAAGGGGTT CTGCGTGCCG GCTCCCAGCT GCGAAATGCC 
GACGTGGAGC TACAGCAGCG GGCCGTGGAG TACCTCACCC TCAGCTCCGT AGCCAGCACC 
GATGTTCTGG CTACGGTGCT AGAAGAAATG CCCCCATTTC CCGAGCGGGA GTCGTCCATC 
TTGGCCAAGC TGAAGCGCAA GAAGGGCCCT GGGGCAGCCA GTGCCCTAGA TGACAGCCGC 
AGGGACACCA GCAGCAATGA CATCAATGGG GGTGTGGAGC CCACCCCCAG CACTGTGTCG 
ACCCCCTCAC CCTCCGCGGA CCTCTTAGGG CTGCGGGCAG CCCCTCCCCC TGCTGCACCC 
CCGGCTCCCG TAGGCGGGAA CCTCCTGGTG GATGTCTTCT CTGACGGCCC CACTGCACAG 
CCCAGCCTGG GGCCCACTCC TGAGGAGGCC TTCCTCAGCG AGCTGGAGCC CCCTGCCCCT 
GAGAGCCCCA TGGCTTTGTT GGCTGACCCA GCTCCAGCTG CTGACCCAGG TCCTGAGGAC 
ATAGGCCCTC CCATTCCAGA AGCAGATGAA CTGTTGAATA AGTTCGTGTG TAAGAATAGT 
GGGGTCTTGT TTGAGAACCA GCTGCTGCAG ATTGGAGTCA AGTCTGAGTT CCGGCAGAAC 
CTGGGCCGCA TGTATCTCTT CTATGGCAAC AAGACTTCTG TGCAGTTCCA GAACTTCTTG 
CCCACCGTGG TCCATCCTGG GGACCTCCAG ACTCAGCTGG CGGTGCAGAC CAAGCGTGTG 
GCGGCACAAG TGGACGGTGG CGCACAGGTG CAGCAAGTAC TCAACATTGA GTGTCTGCGA 
GACTTCCTGA CGCCGCCACT GTTGTCGGTG CGCTTCCGGT ACGGTGGCAC CGCCCAGTCC 
CTCACTCTGA AGCTCCCAGT GACCATCAAC AAATTCTTCC AGCCCACAGA GATGGCGGCC 
CAAGACTTTT TCCAGCGCTG GAAGCAGCTG AGCCTCCCCC TGCAGGAGGC ACAGAAAATC 
TTCAAAGCCA ACCACCCGAT GGATGCTGAA GTTACTAAGG CCAAGCTTCT GGGGTTTGGC 
TCTGCTCTTC TGGACAATGT GGATCCCAAC CCTGAGAACT TTGTGGGTGC TGGAATCATC 
CAGACGAAGG CCCTGCAGGT GGGGTGTCTG CTTCGGCTGG AGCCCAATGC CCAGGCCCAA 
ATGTACCGTC TAACCCTGCG CACCAGCAAA GAGCCTGTCT CCCGTCACTT GTGTGAGCTG 
CTGGCCCAGC AGTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006540633.1
CDS	638..2515
Translation	MCTLASSEFSHEAVKTHIDTVINALKTERDVSVRQRAADLLYAMCDRSNAKQIVSEMLRYLETADYAIREEIVLKVAILAEKYAVDYSWYVDTILNLIRIAGDYVSEEVWYRVLQIVTNRDDVQGYAAKTVFEALQAPACHENMVKVGGYILGEFGNLIAGDPRSSPPVQFSLLHSKFHLCSVATRALLLSTYIKFINLFPETKATIQGVLRAGSQLRNADVELQQRAVEYLTLSSVASTDVLATVLEEMPPFPERESSILAKLKRKKGPGAASALDDSRRDTSSNDINGGVEPTPSTVSTPSPSADLLGLRAAPPPAAPPAPVGGNLLVDVFSDGPTAQPSLGPTPEEAFLSELEPPAPESPMALLADPAPAADPGPEDIGPPIPEADELLNKFVCKNSGVLFENQLLQIGVKSEFRQNLGRMYLFYGNKTSVQFQNFLPTVVHPGDLQTQLAVQTKRVAAQVDGGAQVQQVLNIECLRDFLTPPLLSVRFRYGGTAQSLTLKLPVTINKFFQPTEMAAQDFFQRWKQLSLPLQEAQKIFKANHPMDAEVTKAKLLGFGSALLDNVDPNPENFVGAGIIQTKALQVGCLLRLEPNAQAQMYRLTLRTSKEPVSRHLCELLAQQF

Target ORF information:

RefSeq Version	XM_006540570.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus adaptor-related protein complex 2, alpha 1 subunit (Ap2a1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006540570.1

ORF Insert Sequence:

ATGTGCACGC TGGCCAGCTC CGAGTTCTCC CACGAGGCCG TCAAGACCCA CATTGATACA 
GTTATTAATG CCCTCAAGAC GGAGCGGGAC GTCAGTGTGA GGCAGCGGGC GGCTGATCTC 
CTGTATGCCA TGTGTGACCG GAGCAATGCC AAGCAGATTG TGTCAGAGAT GCTGCGGTAC 
CTGGAGACTG CTGACTATGC CATCCGAGAG GAGATCGTGC TGAAGGTGGC CATCCTGGCT 
GAGAAGTATG CAGTGGACTA CAGCTGGTAC GTGGACACCA TCCTCAACCT CATCCGCATC 
GCGGGCGACT ATGTGAGCGA GGAGGTGTGG TACCGCGTGC TGCAGATCGT CACCAACCGT 
GATGACGTCC AGGGTTATGC TGCCAAGACA GTGTTTGAGG CCCTCCAGGC CCCAGCCTGT 
CATGAGAACA TGGTGAAGGT CGGTGGCTAC ATCCTTGGGG AGTTTGGGAA CTTGATTGCT 
GGGGACCCAC GCTCCAGCCC ACCAGTGCAG TTCTCGCTGC TGCACTCCAA GTTCCACCTG 
TGCAGCGTGG CCACCCGCGC TCTGTTGCTG TCCACCTACA TCAAGTTCAT CAACCTCTTC 
CCTGAGACCA AGGCCACCAT CCAAGGGGTT CTGCGTGCCG GCTCCCAGCT GCGAAATGCC 
GACGTGGAGC TACAGCAGCG GGCCGTGGAG TACCTCACCC TCAGCTCCGT AGCCAGCACC 
GATGTTCTGG CTACGGTGCT AGAAGAAATG CCCCCATTTC CCGAGCGGGA GTCGTCCATC 
TTGGCCAAGC TGAAGCGCAA GAAGGGCCCT GGGGCAGCCA GTGCCCTAGA TGACAGCCGC 
AGGGACACCA GCAGCAATGA CATCAATGGG GGTGTGGAGC CCACCCCCAG CACTGTGTCG 
ACCCCCTCAC CCTCCGCGGA CCTCTTAGGG CTGCGGGCAG CCCCTCCCCC TGCTGCACCC 
CCGGCTCCCG TAGGCGGGAA CCTCCTGGTG GATGTCTTCT CTGACGGCCC CACTGCACAG 
CCCAGCCTGG GGCCCACTCC TGAGGAGGCC TTCCTCAGCG AGCTGGAGCC CCCTGCCCCT 
GAGAGCCCCA TGGCTTTGTT GGCTGACCCA GCTCCAGCTG CTGACCCAGG TCCTGAGGAC 
ATAGGCCCTC CCATTCCAGA AGCAGATGAA CTGTTGAATA AGTTCGTGTG TAAGAATAGT 
GGGGTCTTGT TTGAGAACCA GCTGCTGCAG ATTGGAGTCA AGTCTGAGTT CCGGCAGAAC 
CTGGGCCGCA TGTATCTCTT CTATGGCAAC AAGACTTCTG TGCAGTTCCA GAACTTCTTG 
CCCACCGTGG TCCATCCTGG GGACCTCCAG ACTCAGCTGG CGGTGCAGAC CAAGCGTGTG 
GCGGCACAAG TGGACGGTGG CGCACAGGTG CAGCAAGTAC TCAACATTGA GTGTCTGCGA 
GACTTCCTGA CGCCGCCACT GTTGTCGGTG CGCTTCCGGT ACGGTGGCAC CGCCCAGTCC 
CTCACTCTGA AGCTCCCAGT GACCATCAAC AAATTCTTCC AGCCCACAGA GATGGCGGCC 
CAAGACTTTT TCCAGCGCTG GAAGCAGCTG AGCCTCCCCC TGCAGGAGGC ACAGAAAATC 
TTCAAAGCCA ACCACCCGAT GGATGCTGAA GTTACTAAGG CCAAGCTTCT GGGGTTTGGC 
TCTGCTCTTC TGGACAATGT GGATCCCAAC CCTGAGAACT TTGTGGGTGC TGGAATCATC 
CAGACGAAGG CCCTGCAGGT GGGGTGTCTG CTTCGGCTGG AGCCCAATGC CCAGGCCCAA 
ATGTACCGTC TAACCCTGCG CACCAGCAAA GAGCCTGTCT CCCGTCACTT GTGTGAGCTG 
CTGGCCCAGC AGTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ap2a1 ( NM_001077264.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001070732.1 Mus musculus adaptor-related protein complex 2, alpha 1 subunit (Ap2a1), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OMu20722
Clone ID Related Accession (Same CDS sequence)	NM_001077264.1
Accession Version	NM_001077264.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2868bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-24
Organism	Mus musculus(house mouse)
Product	AP-2 complex subunit alpha-1 isoform b
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from CA327188.1, AK135424.1 and BC031433.1. Transcript Variant: This variant (2) lacks an alternate exon in the coding region compared to variant 1. The resulting protein (isoform b) is shorter but has the same N- and C-termini compared to isoform a. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK135424.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164139 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCCGGCTG TATCCAAAGG CGATGGCATG CGTGGGCTCG CCGTGTTCAT CTCCGACATC 
CGGAACTGCA AGAGCAAAGA GGCTGAGATC AAGAGGATCA ACAAGGAACT GGCCAACATC 
CGTTCCAAGT TCAAAGGGGA CAAGGCCTTG GATGGCTACA GTAAAAAGAA GTATGTGTGT 
AAGCTGCTCT TCATATTCCT GCTTGGCCAT GACATTGACT TTGGACATAT GGAGGCCGTG 
AACCTGCTAA GCTCTAACAA GTACACGGAG AAGCAGATAG GGTACCTGTT CATCTCAGTC 
CTGGTGAACT CGAACTCAGA ACTGATCCGG CTCATCAACA ACGCTATCAA GAATGACCTG 
GCCAGTCGCA ACCCCACCTT CATGTGCCTG GCCTTGCACT GTATCGCTAA CGTGGGCAGC 
CGTGAGATGG GCGAGGCTTT TGCTGCAGAC ATTCCCCGAA TCCTGGTGGC TGGGGACAGC 
ATGGACAGTG TGAAGCAGAG TGCCGCCCTA TGCCTACTGC GACTCTACAA GGCCTCGCCC 
GACTTGGTGC CCATGGGCGA GTGGACGGCA CGTGTAGTGC ACTTGCTCAA TGATCAGCAC 
ATGGGAGTGG TCACAGCTGC TGTCAGCCTC ATCACCTGTC TCTGCAAGAA GAATCCGGAT 
GACTTCAAGA CCTGTATCTC CCTGGCTGTG TCTCGCTTAA GCCGGATCGT CTCCTCAGCC 
TCCACTGACC TCCAGGACTA CACTTACTAC TTCGTTCCTG CACCCTGGCT CTCTGTGAAG 
CTACTGCGGC TGCTCCAGTG TTACCCACCA CCAGAGGATG CAGCCGTGAA AGGGCGGTTA 
GTGGAGTGTC TGGAGACTGT GCTCAACAAG GCCCAGGAGC CTCCCAAGTC CAAGAAGGTG 
CAGCACTCCA ACGCCAAGAA CGCTATCCTC TTTGAGACCA TTAGCCTCAT CATCCACTAT 
GACAGTGAGC CCAACCTCCT GGTCCGCGCC TGCAACCAGC TGGGCCAGTT CCTGCAGCAC 
CGGGAGACTA ACCTGCGCTA CCTGGCCCTG GAGAGCATGT GCACGCTGGC CAGCTCCGAG 
TTCTCCCACG AGGCCGTCAA GACCCACATT GATACAGTTA TTAATGCCCT CAAGACGGAG 
CGGGACGTCA GTGTGAGGCA GCGGGCGGCT GATCTCCTGT ATGCCATGTG TGACCGGAGC 
AATGCCAAGC AGATTGTGTC AGAGATGCTG CGGTACCTGG AGACTGCTGA CTATGCCATC 
CGAGAGGAGA TCGTGCTGAA GGTGGCCATC CTGGCTGAGA AGTATGCAGT GGACTACAGC 
TGGTACGTGG ACACCATCCT CAACCTCATC CGCATCGCGG GCGACTATGT GAGCGAGGAG 
GTGTGGTACC GCGTGCTGCA GATCGTCACC AACCGTGATG ACGTCCAGGG TTATGCTGCC 
AAGACAGTGT TTGAGGCCCT CCAGGCCCCA GCCTGTCATG AGAACATGGT GAAGGTCGGT 
GGCTACATCC TTGGGGAGTT TGGGAACTTG ATTGCTGGGG ACCCACGCTC CAGCCCACCA 
GTGCAGTTCT CGCTGCTGCA CTCCAAGTTC CACCTGTGCA GCGTGGCCAC CCGCGCTCTG 
TTGCTGTCCA CCTACATCAA GTTCATCAAC CTCTTCCCTG AGACCAAGGC CACCATCCAA 
GGGGTTCTGC GTGCCGGCTC CCAGCTGCGA AATGCCGACG TGGAGCTACA GCAGCGGGCC 
GTGGAGTACC TCACCCTCAG CTCCGTAGCC AGCACCGATG TTCTGGCTAC GGTGCTAGAA 
GAAATGCCCC CATTTCCCGA GCGGGAGTCG TCCATCTTGG CCAAGCTGAA GCGCAAGAAG 
GGCCCTGGGG CAGCCAGTGC CCTAGATGAC AGCCGCAGGG ACACCAGCAG CAATGACATC 
AATGGGGGTG TGGAGCCCAC CCCCAGCACT GTGTCGACCC CCTCACCCTC CGCGGACCTC 
TTAGGGCTGC GGGCAGCCCC TCCCCCTGCT GCACCCCCGG CTCCCGTAGG CGGGAACCTC 
CTGGTGGATG TCTTCTCTGA CGGCCCCACT GCACAGCCCA GCCTGGGGCC CACTCCTGAG 
GAGGCCTTCC TCAGCCCAGG TCCTGAGGAC ATAGGCCCTC CCATTCCAGA AGCAGATGAA 
CTGTTGAATA AGTTCGTGTG TAAGAATAGT GGGGTCTTGT TTGAGAACCA GCTGCTGCAG 
ATTGGAGTCA AGTCTGAGTT CCGGCAGAAC CTGGGCCGCA TGTATCTCTT CTATGGCAAC 
AAGACTTCTG TGCAGTTCCA GAACTTCTTG CCCACCGTGG TCCATCCTGG GGACCTCCAG 
ACTCAGCTGG CGGTGCAGAC CAAGCGTGTG GCGGCACAAG TGGACGGTGG CGCACAGGTG 
CAGCAAGTAC TCAACATTGA GTGTCTGCGA GACTTCCTGA CGCCGCCACT GTTGTCGGTG 
CGCTTCCGGT ACGGTGGCAC CGCCCAGTCC CTCACTCTGA AGCTCCCAGT GACCATCAAC 
AAATTCTTCC AGCCCACAGA GATGGCGGCC CAAGACTTTT TCCAGCGCTG GAAGCAGCTG 
AGCCTCCCCC TGCAGGAGGC ACAGAAAATC TTCAAAGCCA ACCACCCGAT GGATGCTGAA 
GTTACTAAGG CCAAGCTTCT GGGGTTTGGC TCTGCTCTTC TGGACAATGT GGATCCCAAC 
CCTGAGAACT TTGTGGGTGC TGGAATCATC CAGACGAAGG CCCTGCAGGT GGGGTGTCTG 
CTTCGGCTGG AGCCCAATGC CCAGGCCCAA ATGTACCGTC TAACCCTGCG CACCAGCAAA 
GAGCCTGTCT CCCGTCACTT GTGTGAGCTG CTGGCCCAGC AGTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001070732.1
CDS	145..3012
Translation	MPAVSKGDGMRGLAVFISDIRNCKSKEAEIKRINKELANIRSKFKGDKALDGYSKKKYVCKLLFIFLLGHDIDFGHMEAVNLLSSNKYTEKQIGYLFISVLVNSNSELIRLINNAIKNDLASRNPTFMCLALHCIANVGSREMGEAFAADIPRILVAGDSMDSVKQSAALCLLRLYKASPDLVPMGEWTARVVHLLNDQHMGVVTAAVSLITCLCKKNPDDFKTCISLAVSRLSRIVSSASTDLQDYTYYFVPAPWLSVKLLRLLQCYPPPEDAAVKGRLVECLETVLNKAQEPPKSKKVQHSNAKNAILFETISLIIHYDSEPNLLVRACNQLGQFLQHRETNLRYLALESMCTLASSEFSHEAVKTHIDTVINALKTERDVSVRQRAADLLYAMCDRSNAKQIVSEMLRYLETADYAIREEIVLKVAILAEKYAVDYSWYVDTILNLIRIAGDYVSEEVWYRVLQIVTNRDDVQGYAAKTVFEALQAPACHENMVKVGGYILGEFGNLIAGDPRSSPPVQFSLLHSKFHLCSVATRALLLSTYIKFINLFPETKATIQGVLRAGSQLRNADVELQQRAVEYLTLSSVASTDVLATVLEEMPPFPERESSILAKLKRKKGPGAASALDDSRRDTSSNDINGGVEPTPSTVSTPSPSADLLGLRAAPPPAAPPAPVGGNLLVDVFSDGPTAQPSLGPTPEEAFLSPGPEDIGPPIPEADELLNKFVCKNSGVLFENQLLQIGVKSEFRQNLGRMYLFYGNKTSVQFQNFLPTVVHPGDLQTQLAVQTKRVAAQVDGGAQVQQVLNIECLRDFLTPPLLSVRFRYGGTAQSLTLKLPVTINKFFQPTEMAAQDFFQRWKQLSLPLQEAQKIFKANHPMDAEVTKAKLLGFGSALLDNVDPNPENFVGAGIIQTKALQVGCLLRLEPNAQAQMYRLTLRTSKEPVSRHLCELLAQQF

Target ORF information:

RefSeq Version	NM_001077264.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus adaptor-related protein complex 2, alpha 1 subunit (Ap2a1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001077264.1

ORF Insert Sequence:

ATGCCGGCTG TATCCAAAGG CGATGGCATG CGTGGGCTCG CCGTGTTCAT CTCCGACATC 
CGGAACTGCA AGAGCAAAGA GGCTGAGATC AAGAGGATCA ACAAGGAACT GGCCAACATC 
CGTTCCAAGT TCAAAGGGGA CAAGGCCTTG GATGGCTACA GTAAAAAGAA GTATGTGTGT 
AAGCTGCTCT TCATATTCCT GCTTGGCCAT GACATTGACT TTGGACATAT GGAGGCCGTG 
AACCTGCTAA GCTCTAACAA GTACACGGAG AAGCAGATAG GGTACCTGTT CATCTCAGTC 
CTGGTGAACT CGAACTCAGA ACTGATCCGG CTCATCAACA ACGCTATCAA GAATGACCTG 
GCCAGTCGCA ACCCCACCTT CATGTGCCTG GCCTTGCACT GTATCGCTAA CGTGGGCAGC 
CGTGAGATGG GCGAGGCTTT TGCTGCAGAC ATTCCCCGAA TCCTGGTGGC TGGGGACAGC 
ATGGACAGTG TGAAGCAGAG TGCCGCCCTA TGCCTACTGC GACTCTACAA GGCCTCGCCC 
GACTTGGTGC CCATGGGCGA GTGGACGGCA CGTGTAGTGC ACTTGCTCAA TGATCAGCAC 
ATGGGAGTGG TCACAGCTGC TGTCAGCCTC ATCACCTGTC TCTGCAAGAA GAATCCGGAT 
GACTTCAAGA CCTGTATCTC CCTGGCTGTG TCTCGCTTAA GCCGGATCGT CTCCTCAGCC 
TCCACTGACC TCCAGGACTA CACTTACTAC TTCGTTCCTG CACCCTGGCT CTCTGTGAAG 
CTACTGCGGC TGCTCCAGTG TTACCCACCA CCAGAGGATG CAGCCGTGAA AGGGCGGTTA 
GTGGAGTGTC TGGAGACTGT GCTCAACAAG GCCCAGGAGC CTCCCAAGTC CAAGAAGGTG 
CAGCACTCCA ACGCCAAGAA CGCTATCCTC TTTGAGACCA TTAGCCTCAT CATCCACTAT 
GACAGTGAGC CCAACCTCCT GGTCCGCGCC TGCAACCAGC TGGGCCAGTT CCTGCAGCAC 
CGGGAGACTA ACCTGCGCTA CCTGGCCCTG GAGAGCATGT GCACGCTGGC CAGCTCCGAG 
TTCTCCCACG AGGCCGTCAA GACCCACATT GATACAGTTA TTAATGCCCT CAAGACGGAG 
CGGGACGTCA GTGTGAGGCA GCGGGCGGCT GATCTCCTGT ATGCCATGTG TGACCGGAGC 
AATGCCAAGC AGATTGTGTC AGAGATGCTG CGGTACCTGG AGACTGCTGA CTATGCCATC 
CGAGAGGAGA TCGTGCTGAA GGTGGCCATC CTGGCTGAGA AGTATGCAGT GGACTACAGC 
TGGTACGTGG ACACCATCCT CAACCTCATC CGCATCGCGG GCGACTATGT GAGCGAGGAG 
GTGTGGTACC GCGTGCTGCA GATCGTCACC AACCGTGATG ACGTCCAGGG TTATGCTGCC 
AAGACAGTGT TTGAGGCCCT CCAGGCCCCA GCCTGTCATG AGAACATGGT GAAGGTCGGT 
GGCTACATCC TTGGGGAGTT TGGGAACTTG ATTGCTGGGG ACCCACGCTC CAGCCCACCA 
GTGCAGTTCT CGCTGCTGCA CTCCAAGTTC CACCTGTGCA GCGTGGCCAC CCGCGCTCTG 
TTGCTGTCCA CCTACATCAA GTTCATCAAC CTCTTCCCTG AGACCAAGGC CACCATCCAA 
GGGGTTCTGC GTGCCGGCTC CCAGCTGCGA AATGCCGACG TGGAGCTACA GCAGCGGGCC 
GTGGAGTACC TCACCCTCAG CTCCGTAGCC AGCACCGATG TTCTGGCTAC GGTGCTAGAA 
GAAATGCCCC CATTTCCCGA GCGGGAGTCG TCCATCTTGG CCAAGCTGAA GCGCAAGAAG 
GGCCCTGGGG CAGCCAGTGC CCTAGATGAC AGCCGCAGGG ACACCAGCAG CAATGACATC 
AATGGGGGTG TGGAGCCCAC CCCCAGCACT GTGTCGACCC CCTCACCCTC CGCGGACCTC 
TTAGGGCTGC GGGCAGCCCC TCCCCCTGCT GCACCCCCGG CTCCCGTAGG CGGGAACCTC 
CTGGTGGATG TCTTCTCTGA CGGCCCCACT GCACAGCCCA GCCTGGGGCC CACTCCTGAG 
GAGGCCTTCC TCAGCCCAGG TCCTGAGGAC ATAGGCCCTC CCATTCCAGA AGCAGATGAA 
CTGTTGAATA AGTTCGTGTG TAAGAATAGT GGGGTCTTGT TTGAGAACCA GCTGCTGCAG 
ATTGGAGTCA AGTCTGAGTT CCGGCAGAAC CTGGGCCGCA TGTATCTCTT CTATGGCAAC 
AAGACTTCTG TGCAGTTCCA GAACTTCTTG CCCACCGTGG TCCATCCTGG GGACCTCCAG 
ACTCAGCTGG CGGTGCAGAC CAAGCGTGTG GCGGCACAAG TGGACGGTGG CGCACAGGTG 
CAGCAAGTAC TCAACATTGA GTGTCTGCGA GACTTCCTGA CGCCGCCACT GTTGTCGGTG 
CGCTTCCGGT ACGGTGGCAC CGCCCAGTCC CTCACTCTGA AGCTCCCAGT GACCATCAAC 
AAATTCTTCC AGCCCACAGA GATGGCGGCC CAAGACTTTT TCCAGCGCTG GAAGCAGCTG 
AGCCTCCCCC TGCAGGAGGC ACAGAAAATC TTCAAAGCCA ACCACCCGAT GGATGCTGAA 
GTTACTAAGG CCAAGCTTCT GGGGTTTGGC TCTGCTCTTC TGGACAATGT GGATCCCAAC 
CCTGAGAACT TTGTGGGTGC TGGAATCATC CAGACGAAGG CCCTGCAGGT GGGGTGTCTG 
CTTCGGCTGG AGCCCAATGC CCAGGCCCAA ATGTACCGTC TAACCCTGCG CACCAGCAAA 
GAGCCTGTCT CCCGTCACTT GTGTGAGCTG CTGGCCCAGC AGTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ap2a1 ( NM_007458.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_031484.1 Mus musculus adaptor-related protein complex 2, alpha 1 subunit (Ap2a1), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OMu20235
Clone ID Related Accession (Same CDS sequence)	NM_007458.2
Accession Version	NM_007458.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2934bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-24
Organism	Mus musculus(house mouse)
Product	AP-2 complex subunit alpha-1 isoform a
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from CA327188.1 and BC031433.1. On Oct 18, 2006 this sequence version replaced NM_007458.1. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer protein (isoform a). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC031433.1, SRR1660811.104228.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCCGGCTG TATCCAAAGG CGATGGCATG CGTGGGCTCG CCGTGTTCAT CTCCGACATC 
CGGAACTGCA AGAGCAAAGA GGCTGAGATC AAGAGGATCA ACAAGGAACT GGCCAACATC 
CGTTCCAAGT TCAAAGGGGA CAAGGCCTTG GATGGCTACA GTAAAAAGAA GTATGTGTGT 
AAGCTGCTCT TCATATTCCT GCTTGGCCAT GACATTGACT TTGGACATAT GGAGGCCGTG 
AACCTGCTAA GCTCTAACAA GTACACGGAG AAGCAGATAG GGTACCTGTT CATCTCAGTC 
CTGGTGAACT CGAACTCAGA ACTGATCCGG CTCATCAACA ACGCTATCAA GAATGACCTG 
GCCAGTCGCA ACCCCACCTT CATGTGCCTG GCCTTGCACT GTATCGCTAA CGTGGGCAGC 
CGTGAGATGG GCGAGGCTTT TGCTGCAGAC ATTCCCCGAA TCCTGGTGGC TGGGGACAGC 
ATGGACAGTG TGAAGCAGAG TGCCGCCCTA TGCCTACTGC GACTCTACAA GGCCTCGCCC 
GACTTGGTGC CCATGGGCGA GTGGACGGCA CGTGTAGTGC ACTTGCTCAA TGATCAGCAC 
ATGGGAGTGG TCACAGCTGC TGTCAGCCTC ATCACCTGTC TCTGCAAGAA GAATCCGGAT 
GACTTCAAGA CCTGTATCTC CCTGGCTGTG TCTCGCTTAA GCCGGATCGT CTCCTCAGCC 
TCCACTGACC TCCAGGACTA CACTTACTAC TTCGTTCCTG CACCCTGGCT CTCTGTGAAG 
CTACTGCGGC TGCTCCAGTG TTACCCACCA CCAGAGGATG CAGCCGTGAA AGGGCGGTTA 
GTGGAGTGTC TGGAGACTGT GCTCAACAAG GCCCAGGAGC CTCCCAAGTC CAAGAAGGTG 
CAGCACTCCA ACGCCAAGAA CGCTATCCTC TTTGAGACCA TTAGCCTCAT CATCCACTAT 
GACAGTGAGC CCAACCTCCT GGTCCGCGCC TGCAACCAGC TGGGCCAGTT CCTGCAGCAC 
CGGGAGACTA ACCTGCGCTA CCTGGCCCTG GAGAGCATGT GCACGCTGGC CAGCTCCGAG 
TTCTCCCACG AGGCCGTCAA GACCCACATT GATACAGTTA TTAATGCCCT CAAGACGGAG 
CGGGACGTCA GTGTGAGGCA GCGGGCGGCT GATCTCCTGT ATGCCATGTG TGACCGGAGC 
AATGCCAAGC AGATTGTGTC AGAGATGCTG CGGTACCTGG AGACTGCTGA CTATGCCATC 
CGAGAGGAGA TCGTGCTGAA GGTGGCCATC CTGGCTGAGA AGTATGCAGT GGACTACAGC 
TGGTACGTGG ACACCATCCT CAACCTCATC CGCATCGCGG GCGACTATGT GAGCGAGGAG 
GTGTGGTACC GCGTGCTGCA GATCGTCACC AACCGTGATG ACGTCCAGGG TTATGCTGCC 
AAGACAGTGT TTGAGGCCCT CCAGGCCCCA GCCTGTCATG AGAACATGGT GAAGGTCGGT 
GGCTACATCC TTGGGGAGTT TGGGAACTTG ATTGCTGGGG ACCCACGCTC CAGCCCACCA 
GTGCAGTTCT CGCTGCTGCA CTCCAAGTTC CACCTGTGCA GCGTGGCCAC CCGCGCTCTG 
TTGCTGTCCA CCTACATCAA GTTCATCAAC CTCTTCCCTG AGACCAAGGC CACCATCCAA 
GGGGTTCTGC GTGCCGGCTC CCAGCTGCGA AATGCCGACG TGGAGCTACA GCAGCGGGCC 
GTGGAGTACC TCACCCTCAG CTCCGTAGCC AGCACCGATG TTCTGGCTAC GGTGCTAGAA 
GAAATGCCCC CATTTCCCGA GCGGGAGTCG TCCATCTTGG CCAAGCTGAA GCGCAAGAAG 
GGCCCTGGGG CAGCCAGTGC CCTAGATGAC AGCCGCAGGG ACACCAGCAG CAATGACATC 
AATGGGGGTG TGGAGCCCAC CCCCAGCACT GTGTCGACCC CCTCACCCTC CGCGGACCTC 
TTAGGGCTGC GGGCAGCCCC TCCCCCTGCT GCACCCCCGG CTCCCGTAGG CGGGAACCTC 
CTGGTGGATG TCTTCTCTGA CGGCCCCACT GCACAGCCCA GCCTGGGGCC CACTCCTGAG 
GAGGCCTTCC TCAGCGAGCT GGAGCCCCCT GCCCCTGAGA GCCCCATGGC TTTGTTGGCT 
GACCCAGCTC CAGCTGCTGA CCCAGGTCCT GAGGACATAG GCCCTCCCAT TCCAGAAGCA 
GATGAACTGT TGAATAAGTT CGTGTGTAAG AATAGTGGGG TCTTGTTTGA GAACCAGCTG 
CTGCAGATTG GAGTCAAGTC TGAGTTCCGG CAGAACCTGG GCCGCATGTA TCTCTTCTAT 
GGCAACAAGA CTTCTGTGCA GTTCCAGAAC TTCTTGCCCA CCGTGGTCCA TCCTGGGGAC 
CTCCAGACTC AGCTGGCGGT GCAGACCAAG CGTGTGGCGG CACAAGTGGA CGGTGGCGCA 
CAGGTGCAGC AAGTACTCAA CATTGAGTGT CTGCGAGACT TCCTGACGCC GCCACTGTTG 
TCGGTGCGCT TCCGGTACGG TGGCACCGCC CAGTCCCTCA CTCTGAAGCT CCCAGTGACC 
ATCAACAAAT TCTTCCAGCC CACAGAGATG GCGGCCCAAG ACTTTTTCCA GCGCTGGAAG 
CAGCTGAGCC TCCCCCTGCA GGAGGCACAG AAAATCTTCA AAGCCAACCA CCCGATGGAT 
GCTGAAGTTA CTAAGGCCAA GCTTCTGGGG TTTGGCTCTG CTCTTCTGGA CAATGTGGAT 
CCCAACCCTG AGAACTTTGT GGGTGCTGGA ATCATCCAGA CGAAGGCCCT GCAGGTGGGG 
TGTCTGCTTC GGCTGGAGCC CAATGCCCAG GCCCAAATGT ACCGTCTAAC CCTGCGCACC 
AGCAAAGAGC CTGTCTCCCG TCACTTGTGT GAGCTGCTGG CCCAGCAGTT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_031484.1
CDS	145..3078
Translation	MPAVSKGDGMRGLAVFISDIRNCKSKEAEIKRINKELANIRSKFKGDKALDGYSKKKYVCKLLFIFLLGHDIDFGHMEAVNLLSSNKYTEKQIGYLFISVLVNSNSELIRLINNAIKNDLASRNPTFMCLALHCIANVGSREMGEAFAADIPRILVAGDSMDSVKQSAALCLLRLYKASPDLVPMGEWTARVVHLLNDQHMGVVTAAVSLITCLCKKNPDDFKTCISLAVSRLSRIVSSASTDLQDYTYYFVPAPWLSVKLLRLLQCYPPPEDAAVKGRLVECLETVLNKAQEPPKSKKVQHSNAKNAILFETISLIIHYDSEPNLLVRACNQLGQFLQHRETNLRYLALESMCTLASSEFSHEAVKTHIDTVINALKTERDVSVRQRAADLLYAMCDRSNAKQIVSEMLRYLETADYAIREEIVLKVAILAEKYAVDYSWYVDTILNLIRIAGDYVSEEVWYRVLQIVTNRDDVQGYAAKTVFEALQAPACHENMVKVGGYILGEFGNLIAGDPRSSPPVQFSLLHSKFHLCSVATRALLLSTYIKFINLFPETKATIQGVLRAGSQLRNADVELQQRAVEYLTLSSVASTDVLATVLEEMPPFPERESSILAKLKRKKGPGAASALDDSRRDTSSNDINGGVEPTPSTVSTPSPSADLLGLRAAPPPAAPPAPVGGNLLVDVFSDGPTAQPSLGPTPEEAFLSELEPPAPESPMALLADPAPAADPGPEDIGPPIPEADELLNKFVCKNSGVLFENQLLQIGVKSEFRQNLGRMYLFYGNKTSVQFQNFLPTVVHPGDLQTQLAVQTKRVAAQVDGGAQVQQVLNIECLRDFLTPPLLSVRFRYGGTAQSLTLKLPVTINKFFQPTEMAAQDFFQRWKQLSLPLQEAQKIFKANHPMDAEVTKAKLLGFGSALLDNVDPNPENFVGAGIIQTKALQVGCLLRLEPNAQAQMYRLTLRTSKEPVSRHLCELLAQQF

Target ORF information:

RefSeq Version	NM_007458.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus adaptor-related protein complex 2, alpha 1 subunit (Ap2a1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_007458.2

ORF Insert Sequence:

ATGCCGGCTG TATCCAAAGG CGATGGCATG CGTGGGCTCG CCGTGTTCAT CTCCGACATC 
CGGAACTGCA AGAGCAAAGA GGCTGAGATC AAGAGGATCA ACAAGGAACT GGCCAACATC 
CGTTCCAAGT TCAAAGGGGA CAAGGCCTTG GATGGCTACA GTAAAAAGAA GTATGTGTGT 
AAGCTGCTCT TCATATTCCT GCTTGGCCAT GACATTGACT TTGGACATAT GGAGGCCGTG 
AACCTGCTAA GCTCTAACAA GTACACGGAG AAGCAGATAG GGTACCTGTT CATCTCAGTC 
CTGGTGAACT CGAACTCAGA ACTGATCCGG CTCATCAACA ACGCTATCAA GAATGACCTG 
GCCAGTCGCA ACCCCACCTT CATGTGCCTG GCCTTGCACT GTATCGCTAA CGTGGGCAGC 
CGTGAGATGG GCGAGGCTTT TGCTGCAGAC ATTCCCCGAA TCCTGGTGGC TGGGGACAGC 
ATGGACAGTG TGAAGCAGAG TGCCGCCCTA TGCCTACTGC GACTCTACAA GGCCTCGCCC 
GACTTGGTGC CCATGGGCGA GTGGACGGCA CGTGTAGTGC ACTTGCTCAA TGATCAGCAC 
ATGGGAGTGG TCACAGCTGC TGTCAGCCTC ATCACCTGTC TCTGCAAGAA GAATCCGGAT 
GACTTCAAGA CCTGTATCTC CCTGGCTGTG TCTCGCTTAA GCCGGATCGT CTCCTCAGCC 
TCCACTGACC TCCAGGACTA CACTTACTAC TTCGTTCCTG CACCCTGGCT CTCTGTGAAG 
CTACTGCGGC TGCTCCAGTG TTACCCACCA CCAGAGGATG CAGCCGTGAA AGGGCGGTTA 
GTGGAGTGTC TGGAGACTGT GCTCAACAAG GCCCAGGAGC CTCCCAAGTC CAAGAAGGTG 
CAGCACTCCA ACGCCAAGAA CGCTATCCTC TTTGAGACCA TTAGCCTCAT CATCCACTAT 
GACAGTGAGC CCAACCTCCT GGTCCGCGCC TGCAACCAGC TGGGCCAGTT CCTGCAGCAC 
CGGGAGACTA ACCTGCGCTA CCTGGCCCTG GAGAGCATGT GCACGCTGGC CAGCTCCGAG 
TTCTCCCACG AGGCCGTCAA GACCCACATT GATACAGTTA TTAATGCCCT CAAGACGGAG 
CGGGACGTCA GTGTGAGGCA GCGGGCGGCT GATCTCCTGT ATGCCATGTG TGACCGGAGC 
AATGCCAAGC AGATTGTGTC AGAGATGCTG CGGTACCTGG AGACTGCTGA CTATGCCATC 
CGAGAGGAGA TCGTGCTGAA GGTGGCCATC CTGGCTGAGA AGTATGCAGT GGACTACAGC 
TGGTACGTGG ACACCATCCT CAACCTCATC CGCATCGCGG GCGACTATGT GAGCGAGGAG 
GTGTGGTACC GCGTGCTGCA GATCGTCACC AACCGTGATG ACGTCCAGGG TTATGCTGCC 
AAGACAGTGT TTGAGGCCCT CCAGGCCCCA GCCTGTCATG AGAACATGGT GAAGGTCGGT 
GGCTACATCC TTGGGGAGTT TGGGAACTTG ATTGCTGGGG ACCCACGCTC CAGCCCACCA 
GTGCAGTTCT CGCTGCTGCA CTCCAAGTTC CACCTGTGCA GCGTGGCCAC CCGCGCTCTG 
TTGCTGTCCA CCTACATCAA GTTCATCAAC CTCTTCCCTG AGACCAAGGC CACCATCCAA 
GGGGTTCTGC GTGCCGGCTC CCAGCTGCGA AATGCCGACG TGGAGCTACA GCAGCGGGCC 
GTGGAGTACC TCACCCTCAG CTCCGTAGCC AGCACCGATG TTCTGGCTAC GGTGCTAGAA 
GAAATGCCCC CATTTCCCGA GCGGGAGTCG TCCATCTTGG CCAAGCTGAA GCGCAAGAAG 
GGCCCTGGGG CAGCCAGTGC CCTAGATGAC AGCCGCAGGG ACACCAGCAG CAATGACATC 
AATGGGGGTG TGGAGCCCAC CCCCAGCACT GTGTCGACCC CCTCACCCTC CGCGGACCTC 
TTAGGGCTGC GGGCAGCCCC TCCCCCTGCT GCACCCCCGG CTCCCGTAGG CGGGAACCTC 
CTGGTGGATG TCTTCTCTGA CGGCCCCACT GCACAGCCCA GCCTGGGGCC CACTCCTGAG 
GAGGCCTTCC TCAGCGAGCT GGAGCCCCCT GCCCCTGAGA GCCCCATGGC TTTGTTGGCT 
GACCCAGCTC CAGCTGCTGA CCCAGGTCCT GAGGACATAG GCCCTCCCAT TCCAGAAGCA 
GATGAACTGT TGAATAAGTT CGTGTGTAAG AATAGTGGGG TCTTGTTTGA GAACCAGCTG 
CTGCAGATTG GAGTCAAGTC TGAGTTCCGG CAGAACCTGG GCCGCATGTA TCTCTTCTAT 
GGCAACAAGA CTTCTGTGCA GTTCCAGAAC TTCTTGCCCA CCGTGGTCCA TCCTGGGGAC 
CTCCAGACTC AGCTGGCGGT GCAGACCAAG CGTGTGGCGG CACAAGTGGA CGGTGGCGCA 
CAGGTGCAGC AAGTACTCAA CATTGAGTGT CTGCGAGACT TCCTGACGCC GCCACTGTTG 
TCGGTGCGCT TCCGGTACGG TGGCACCGCC CAGTCCCTCA CTCTGAAGCT CCCAGTGACC 
ATCAACAAAT TCTTCCAGCC CACAGAGATG GCGGCCCAAG ACTTTTTCCA GCGCTGGAAG 
CAGCTGAGCC TCCCCCTGCA GGAGGCACAG AAAATCTTCA AAGCCAACCA CCCGATGGAT 
GCTGAAGTTA CTAAGGCCAA GCTTCTGGGG TTTGGCTCTG CTCTTCTGGA CAATGTGGAT 
CCCAACCCTG AGAACTTTGT GGGTGCTGGA ATCATCCAGA CGAAGGCCCT GCAGGTGGGG 
TGTCTGCTTC GGCTGGAGCC CAATGCCCAG GCCCAAATGT ACCGTCTAAC CCTGCGCACC 
AGCAAAGAGC CTGTCTCCCG TCACTTGTGT GAGCTGCTGG CCCAGCAGTT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ap2a1 ( XM_006540569.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006540632.1 Mus musculus adaptor-related protein complex 2, alpha 1 subunit (Ap2a1), transcript variant X1, mRNA.

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CloneID	OMu38640
Clone ID Related Accession (Same CDS sequence)	XM_006540569.4 , XM_006540569.3
Accession Version	XM_006540569.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2706bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	AP-2 complex subunit alpha-1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000073.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006540569.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGCCG TGAACCTGCT AAGCTCTAAC AAGTACACGG AGAAGCAGAT AGGGTACCTG 
TTCATCTCAG TCCTGGTGAA CTCGAACTCA GAACTGATCC GGCTCATCAA CAACGCTATC 
AAGAATGACC TGGCCAGTCG CAACCCCACC TTCATGTGCC TGGCCTTGCA CTGTATCGCT 
AACGTGGGCA GCCGTGAGAT GGGCGAGGCT TTTGCTGCAG ACATTCCCCG AATCCTGGTG 
GCTGGGGACA GCATGGACAG TGTGAAGCAG AGTGCCGCCC TATGCCTACT GCGACTCTAC 
AAGGCCTCGC CCGACTTGGT GCCCATGGGC GAGTGGACGG CACGTGTAGT GCACTTGCTC 
AATGATCAGC ACATGGGAGT GGTCACAGCT GCTGTCAGCC TCATCACCTG TCTCTGCAAG 
AAGAATCCGG ATGACTTCAA GACCTGTATC TCCCTGGCTG TGTCTCGCTT AAGCCGGATC 
GTCTCCTCAG CCTCCACTGA CCTCCAGGAC TACACTTACT ACTTCGTTCC TGCACCCTGG 
CTCTCTGTGA AGCTACTGCG GCTGCTCCAG TGTTACCCAC CACCAGAGGA TGCAGCCGTG 
AAAGGGCGGT TAGTGGAGTG TCTGGAGACT GTGCTCAACA AGGCCCAGGA GCCTCCCAAG 
TCCAAGAAGG TGCAGCACTC CAACGCCAAG AACGCTATCC TCTTTGAGAC CATTAGCCTC 
ATCATCCACT ATGACAGTGA GCCCAACCTC CTGGTCCGCG CCTGCAACCA GCTGGGCCAG 
TTCCTGCAGC ACCGGGAGAC TAACCTGCGC TACCTGGCCC TGGAGAGCAT GTGCACGCTG 
GCCAGCTCCG AGTTCTCCCA CGAGGCCGTC AAGACCCACA TTGATACAGT TATTAATGCC 
CTCAAGACGG AGCGGGACGT CAGTGTGAGG CAGCGGGCGG CTGATCTCCT GTATGCCATG 
TGTGACCGGA GCAATGCCAA GCAGATTGTG TCAGAGATGC TGCGGTACCT GGAGACTGCT 
GACTATGCCA TCCGAGAGGA GATCGTGCTG AAGGTGGCCA TCCTGGCTGA GAAGTATGCA 
GTGGACTACA GCTGGTACGT GGACACCATC CTCAACCTCA TCCGCATCGC GGGCGACTAT 
GTGAGCGAGG AGGTGTGGTA CCGCGTGCTG CAGATCGTCA CCAACCGTGA TGACGTCCAG 
GGTTATGCTG CCAAGACAGT GTTTGAGGCC CTCCAGGCCC CAGCCTGTCA TGAGAACATG 
GTGAAGGTCG GTGGCTACAT CCTTGGGGAG TTTGGGAACT TGATTGCTGG GGACCCACGC 
TCCAGCCCAC CAGTGCAGTT CTCGCTGCTG CACTCCAAGT TCCACCTGTG CAGCGTGGCC 
ACCCGCGCTC TGTTGCTGTC CACCTACATC AAGTTCATCA ACCTCTTCCC TGAGACCAAG 
GCCACCATCC AAGGGGTTCT GCGTGCCGGC TCCCAGCTGC GAAATGCCGA CGTGGAGCTA 
CAGCAGCGGG CCGTGGAGTA CCTCACCCTC AGCTCCGTAG CCAGCACCGA TGTTCTGGCT 
ACGGTGCTAG AAGAAATGCC CCCATTTCCC GAGCGGGAGT CGTCCATCTT GGCCAAGCTG 
AAGCGCAAGA AGGGCCCTGG GGCAGCCAGT GCCCTAGATG ACAGCCGCAG GGACACCAGC 
AGCAATGACA TCAATGGGGG TGTGGAGCCC ACCCCCAGCA CTGTGTCGAC CCCCTCACCC 
TCCGCGGACC TCTTAGGGCT GCGGGCAGCC CCTCCCCCTG CTGCACCCCC GGCTCCCGTA 
GGCGGGAACC TCCTGGTGGA TGTCTTCTCT GACGGCCCCA CTGCACAGCC CAGCCTGGGG 
CCCACTCCTG AGGAGGCCTT CCTCAGCGAG CTGGAGCCCC CTGCCCCTGA GAGCCCCATG 
GCTTTGTTGG CTGACCCAGC TCCAGCTGCT GACCCAGGTC CTGAGGACAT AGGCCCTCCC 
ATTCCAGAAG CAGATGAACT GTTGAATAAG TTCGTGTGTA AGAATAGTGG GGTCTTGTTT 
GAGAACCAGC TGCTGCAGAT TGGAGTCAAG TCTGAGTTCC GGCAGAACCT GGGCCGCATG 
TATCTCTTCT ATGGCAACAA GACTTCTGTG CAGTTCCAGA ACTTCTTGCC CACCGTGGTC 
CATCCTGGGG ACCTCCAGAC TCAGCTGGCG GTGCAGACCA AGCGTGTGGC GGCACAAGTG 
GACGGTGGCG CACAGGTGCA GCAAGTACTC AACATTGAGT GTCTGCGAGA CTTCCTGACG 
CCGCCACTGT TGTCGGTGCG CTTCCGGTAC GGTGGCACCG CCCAGTCCCT CACTCTGAAG 
CTCCCAGTGA CCATCAACAA ATTCTTCCAG CCCACAGAGA TGGCGGCCCA AGACTTTTTC 
CAGCGCTGGA AGCAGCTGAG CCTCCCCCTG CAGGAGGCAC AGAAAATCTT CAAAGCCAAC 
CACCCGATGG ATGCTGAAGT TACTAAGGCC AAGCTTCTGG GGTTTGGCTC TGCTCTTCTG 
GACAATGTGG ATCCCAACCC TGAGAACTTT GTGGGTGCTG GAATCATCCA GACGAAGGCC 
CTGCAGGTGG GGTGTCTGCT TCGGCTGGAG CCCAATGCCC AGGCCCAAAT GTACCGTCTA 
ACCCTGCGCA CCAGCAAAGA GCCTGTCTCC CGTCACTTGT GTGAGCTGCT GGCCCAGCAG 
TTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006540632.1
CDS	471..3176
Translation	MEAVNLLSSNKYTEKQIGYLFISVLVNSNSELIRLINNAIKNDLASRNPTFMCLALHCIANVGSREMGEAFAADIPRILVAGDSMDSVKQSAALCLLRLYKASPDLVPMGEWTARVVHLLNDQHMGVVTAAVSLITCLCKKNPDDFKTCISLAVSRLSRIVSSASTDLQDYTYYFVPAPWLSVKLLRLLQCYPPPEDAAVKGRLVECLETVLNKAQEPPKSKKVQHSNAKNAILFETISLIIHYDSEPNLLVRACNQLGQFLQHRETNLRYLALESMCTLASSEFSHEAVKTHIDTVINALKTERDVSVRQRAADLLYAMCDRSNAKQIVSEMLRYLETADYAIREEIVLKVAILAEKYAVDYSWYVDTILNLIRIAGDYVSEEVWYRVLQIVTNRDDVQGYAAKTVFEALQAPACHENMVKVGGYILGEFGNLIAGDPRSSPPVQFSLLHSKFHLCSVATRALLLSTYIKFINLFPETKATIQGVLRAGSQLRNADVELQQRAVEYLTLSSVASTDVLATVLEEMPPFPERESSILAKLKRKKGPGAASALDDSRRDTSSNDINGGVEPTPSTVSTPSPSADLLGLRAAPPPAAPPAPVGGNLLVDVFSDGPTAQPSLGPTPEEAFLSELEPPAPESPMALLADPAPAADPGPEDIGPPIPEADELLNKFVCKNSGVLFENQLLQIGVKSEFRQNLGRMYLFYGNKTSVQFQNFLPTVVHPGDLQTQLAVQTKRVAAQVDGGAQVQQVLNIECLRDFLTPPLLSVRFRYGGTAQSLTLKLPVTINKFFQPTEMAAQDFFQRWKQLSLPLQEAQKIFKANHPMDAEVTKAKLLGFGSALLDNVDPNPENFVGAGIIQTKALQVGCLLRLEPNAQAQMYRLTLRTSKEPVSRHLCELLAQQF

Target ORF information:

RefSeq Version	XM_006540569.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus adaptor-related protein complex 2, alpha 1 subunit (Ap2a1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006540569.4

ORF Insert Sequence:

ATGGAGGCCG TGAACCTGCT AAGCTCTAAC AAGTACACGG AGAAGCAGAT AGGGTACCTG 
TTCATCTCAG TCCTGGTGAA CTCGAACTCA GAACTGATCC GGCTCATCAA CAACGCTATC 
AAGAATGACC TGGCCAGTCG CAACCCCACC TTCATGTGCC TGGCCTTGCA CTGTATCGCT 
AACGTGGGCA GCCGTGAGAT GGGCGAGGCT TTTGCTGCAG ACATTCCCCG AATCCTGGTG 
GCTGGGGACA GCATGGACAG TGTGAAGCAG AGTGCCGCCC TATGCCTACT GCGACTCTAC 
AAGGCCTCGC CCGACTTGGT GCCCATGGGC GAGTGGACGG CACGTGTAGT GCACTTGCTC 
AATGATCAGC ACATGGGAGT GGTCACAGCT GCTGTCAGCC TCATCACCTG TCTCTGCAAG 
AAGAATCCGG ATGACTTCAA GACCTGTATC TCCCTGGCTG TGTCTCGCTT AAGCCGGATC 
GTCTCCTCAG CCTCCACTGA CCTCCAGGAC TACACTTACT ACTTCGTTCC TGCACCCTGG 
CTCTCTGTGA AGCTACTGCG GCTGCTCCAG TGTTACCCAC CACCAGAGGA TGCAGCCGTG 
AAAGGGCGGT TAGTGGAGTG TCTGGAGACT GTGCTCAACA AGGCCCAGGA GCCTCCCAAG 
TCCAAGAAGG TGCAGCACTC CAACGCCAAG AACGCTATCC TCTTTGAGAC CATTAGCCTC 
ATCATCCACT ATGACAGTGA GCCCAACCTC CTGGTCCGCG CCTGCAACCA GCTGGGCCAG 
TTCCTGCAGC ACCGGGAGAC TAACCTGCGC TACCTGGCCC TGGAGAGCAT GTGCACGCTG 
GCCAGCTCCG AGTTCTCCCA CGAGGCCGTC AAGACCCACA TTGATACAGT TATTAATGCC 
CTCAAGACGG AGCGGGACGT CAGTGTGAGG CAGCGGGCGG CTGATCTCCT GTATGCCATG 
TGTGACCGGA GCAATGCCAA GCAGATTGTG TCAGAGATGC TGCGGTACCT GGAGACTGCT 
GACTATGCCA TCCGAGAGGA GATCGTGCTG AAGGTGGCCA TCCTGGCTGA GAAGTATGCA 
GTGGACTACA GCTGGTACGT GGACACCATC CTCAACCTCA TCCGCATCGC GGGCGACTAT 
GTGAGCGAGG AGGTGTGGTA CCGCGTGCTG CAGATCGTCA CCAACCGTGA TGACGTCCAG 
GGTTATGCTG CCAAGACAGT GTTTGAGGCC CTCCAGGCCC CAGCCTGTCA TGAGAACATG 
GTGAAGGTCG GTGGCTACAT CCTTGGGGAG TTTGGGAACT TGATTGCTGG GGACCCACGC 
TCCAGCCCAC CAGTGCAGTT CTCGCTGCTG CACTCCAAGT TCCACCTGTG CAGCGTGGCC 
ACCCGCGCTC TGTTGCTGTC CACCTACATC AAGTTCATCA ACCTCTTCCC TGAGACCAAG 
GCCACCATCC AAGGGGTTCT GCGTGCCGGC TCCCAGCTGC GAAATGCCGA CGTGGAGCTA 
CAGCAGCGGG CCGTGGAGTA CCTCACCCTC AGCTCCGTAG CCAGCACCGA TGTTCTGGCT 
ACGGTGCTAG AAGAAATGCC CCCATTTCCC GAGCGGGAGT CGTCCATCTT GGCCAAGCTG 
AAGCGCAAGA AGGGCCCTGG GGCAGCCAGT GCCCTAGATG ACAGCCGCAG GGACACCAGC 
AGCAATGACA TCAATGGGGG TGTGGAGCCC ACCCCCAGCA CTGTGTCGAC CCCCTCACCC 
TCCGCGGACC TCTTAGGGCT GCGGGCAGCC CCTCCCCCTG CTGCACCCCC GGCTCCCGTA 
GGCGGGAACC TCCTGGTGGA TGTCTTCTCT GACGGCCCCA CTGCACAGCC CAGCCTGGGG 
CCCACTCCTG AGGAGGCCTT CCTCAGCGAG CTGGAGCCCC CTGCCCCTGA GAGCCCCATG 
GCTTTGTTGG CTGACCCAGC TCCAGCTGCT GACCCAGGTC CTGAGGACAT AGGCCCTCCC 
ATTCCAGAAG CAGATGAACT GTTGAATAAG TTCGTGTGTA AGAATAGTGG GGTCTTGTTT 
GAGAACCAGC TGCTGCAGAT TGGAGTCAAG TCTGAGTTCC GGCAGAACCT GGGCCGCATG 
TATCTCTTCT ATGGCAACAA GACTTCTGTG CAGTTCCAGA ACTTCTTGCC CACCGTGGTC 
CATCCTGGGG ACCTCCAGAC TCAGCTGGCG GTGCAGACCA AGCGTGTGGC GGCACAAGTG 
GACGGTGGCG CACAGGTGCA GCAAGTACTC AACATTGAGT GTCTGCGAGA CTTCCTGACG 
CCGCCACTGT TGTCGGTGCG CTTCCGGTAC GGTGGCACCG CCCAGTCCCT CACTCTGAAG 
CTCCCAGTGA CCATCAACAA ATTCTTCCAG CCCACAGAGA TGGCGGCCCA AGACTTTTTC 
CAGCGCTGGA AGCAGCTGAG CCTCCCCCTG CAGGAGGCAC AGAAAATCTT CAAAGCCAAC 
CACCCGATGG ATGCTGAAGT TACTAAGGCC AAGCTTCTGG GGTTTGGCTC TGCTCTTCTG 
GACAATGTGG ATCCCAACCC TGAGAACTTT GTGGGTGCTG GAATCATCCA GACGAAGGCC 
CTGCAGGTGG GGTGTCTGCT TCGGCTGGAG CCCAATGCCC AGGCCCAAAT GTACCGTCTA 
ACCCTGCGCA CCAGCAAAGA GCCTGTCTCC CGTCACTTGT GTGAGCTGCT GGCCCAGCAG 
TTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

APache Is an AP2-Interacting Protein Involved in Synaptic Vesicle Trafficking and Neuronal Development.
Cell reports21(12)3596-3611(2017 Dec)
Piccini A,Castroflorio E,Valente P,Guarnieri FC,Aprile D,Michetti C,Bramini M,Giansante G,Pinto B,Savardi A,Cesca F,Bachi A,Cattaneo A,Wren JD,Fassio A,Valtorta F,Benfenati F,Gioved? S

Surfactant Protein A Enhances Constitutive Immune Functions of Clathrin Heavy Chain and Clathrin Adaptor Protein 2.
American journal of respiratory cell and molecular biology55(1)92-104(2016 Jul)
Moulakakis C,Steinh?user C,Biedziak D,Freundt K,Reiling N,Stamme C

SPECC1L deficiency results in increased adherens junction stability and reduced cranial neural crest cell delamination.
Scientific reports617735(2016 Jan)
Wilson NR,Olm-Shipman AJ,Acevedo DS,Palaniyandi K,Hall EG,Kosa E,Stumpff KM,Smith GJ,Pitstick L,Liao EC,Bjork BC,Czirok A,Saadi I

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Ap2a1 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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