UBE3B cDNA ORF clone, Mus musculus(House mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	UBE3B
Entrez Gene ID	117146
Full Name	ubiquitin protein ligase E3B
Synonyms	AI449831,AU020130
General protein information	Preferred Names ubiquitin protein ligase E3B Names ubiquitin-protein ligase E3B HECT-type ubiquitin transferase E3B
Gene Type	protein-coding
Organism	Mus musculus(House mouse)
Genome	Chromosome: 5 Map Location: 5\|5 F

mRNA and Protein(s)

mRNA	Protein	Name
XM_006530126.2	XP_006530189.1	ubiquitin-protein ligase E3B isoform X1
XM_006530126.3	XP_006530189.1	ubiquitin-protein ligase E3B isoform X1
NM_054093.2	NP_473434.2	ubiquitin-protein ligase E3B
NM_054093.3	NP_473434.2	ubiquitin-protein ligase E3B isoform 1
XM_011248161.2	XP_011246463.1	ubiquitin-protein ligase E3B isoform X2
XM_011248161.1	XP_011246463.1	ubiquitin-protein ligase E3B isoform X2
NM_001331219.1	NP_001318148.1	ubiquitin-protein ligase E3B isoform 2 precursor
XM_006530125.1	XP_006530188.1	ubiquitin-protein ligase E3B isoform X1

Pathways from BioSystems

Homologies

Xenopus tropicalis (tropical clawed frog)	ube3b	NP_001072875.1
Pan troglodytes (chimpanzee)	UBE3B	XP_001137003.2
Danio rerio (zebrafish)	ube3b	NP_001108154.1
	UBE3B	XP_002798818.1
Homo sapiens (human)	UBE3B	NP_569733.2
Canis lupus familiaris (dog)	UBE3B	XP_543437.2
Bos taurus (cattle)	UBE3B	NP_001193161.1
Drosophila melanogaster (fruit fly)	CG5087	NP_001261610.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP005897	XP_315926.2
Caenorhabditis elegans (roundworm)	oxi-1	NP_499392.1
Mus musculus (house mouse)	Ube3b	NP_473434.2
Rattus norvegicus (Norway rat)	Ube3b	NP_001137366.1
Gallus gallus (chicken)	LOC776286	XP_003642246.2

Gene Ontology
Bibliography

Related articles in PubMed

Deficiency for the ubiquitin ligase UBE3B in a blepharophimosis-ptosis-intellectual-disability syndrome.
Basel-Vanagaite L, Dallapiccola B, Ramirez-Solis R, Segref A, Thiele H, Edwards A, Arends MJ, Mir? X, White JK, D?sir J, Abramowicz M, Dentici ML, Lepri F, Hofmann K, Har-Zahav A, Ryder E, Karp NA, Estabel J, Gerdin AK, Podrini C, Ingham NJ, Altm?ller J, N?rnberg G, Frommolt P, Abdelhak S, Pasmanik-Chor M, Konen O, Kelley RI, Shohat M, N?rnberg P, Flint J, Steel KP, Hoppe T, Kubisch C, Adams DJ, Borck G
American journal of human genetics91(6)998-1010(2012 Dec)

Characterization of the human UBE3B gene: structure, expression, evolution, and alternative splicing.
Gong TW, Huang L, Warner SJ, Lomax MI
Genomics82(2)143-52(2003 Aug)

Differential display and gene arrays to examine auditory plasticity.
Lomax MI, Huang L, Cho Y, Gong TL, Altschuler RA
Hearing research147(1-2)293-302(2000 Sep)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Our data reveal the pleiotropic effects of UBE3B deficiency and reinforce the physiological importance of ubiquitination in neuronal development and function in mammals.
Title: Deficiency for the ubiquitin ligase UBE3B in a blepharophimosis-ptosis-intellectual-disability syndrome.

The following UBE3B gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the UBE3B cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu83388	NM_001331219.1 Latest version!	Mus musculus ubiquitin protein ligase E3B (Ube3b), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	10-12	$475.30 $679.00
OMu72445	XM_011248161.2 Latest version!	Mus musculus ubiquitin protein ligase E3B (Ube3b), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
OMu09583	NM_054093.3 Latest version!	Mus musculus ubiquitin protein ligase E3B (Ube3b), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	16	$559.30 $799.00
OMu09583	XM_006530125.1 Latest version!	Mus musculus ubiquitin protein ligase E3B (Ube3b), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	16	$559.30 $799.00
OMu35771	XM_006530126.3 Latest version!	Mus musculus ubiquitin protein ligase E3B (Ube3b), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
View Old Accession Versions >>
OMu35771	XM_006530126.2	Mus musculus ubiquitin protein ligase E3B (Ube3b), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00
OMu09583	NM_054093.2	Mus musculus ubiquitin protein ligase E3B (Ube3b), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	16	$559.30 $799.00
OMu72445	XM_011248161.1	Mus musculus ubiquitin protein ligase E3B (Ube3b), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$307.30 $439.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

UBE3B ( XM_011248161.1 ) cDNA ORF clone, Mus musculus(House mouse) -> XP_011246463.1 Mus musculus ubiquitin protein ligase E3B (Ube3b), transcript variant X3, mRNA.

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CloneID	OMu72445
Clone ID Related Accession (Same CDS sequence)	XM_011248161.2 , XM_011248161.1
Accession Version	XM_011248161.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1626bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	ubiquitin-protein ligase E3B isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000071.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGATGC TCTTCTGTGA CTGTTCCCGG CACCTCATCA CCATCCTCGA TGACATTGAA 
GTTTATGAAG AACAGATCTC CTTCAAGCTG GAAGAGCTGG TCACCATCTC CTCGTTCCTC 
AATTCCTTCG TGTTTAAGAT GATCTGGGAC GGAATTGTAG AGAATGCCAA GGGTGAGACC 
TTGGAGCTGT TCCAGTCCGT CCATGGCTGG CTGATGGTGC TGTATGAGCG AGACTGCCGC 
CGGCGCTTTG CTCCGGAGGA CCACTGGCTA CGGAGGGACC TCAAGCCTGG TGTGCTCTTC 
CAGGAGCTCG ACAAGGACCG AAGGAGGGCT CAGCTGGTCC TGCAGCACAT CCCCCACGTC 
GTGCCTCACA AGAACAGAGT TCTTCTGTTC CGGAACATGG TTATCAAGGA GAAAGAGAAG 
CTGGGGCTTG TGGAGACCAG CTCTGCCTCT CCTCACGTCA CCCACATCAC CATCCGAAGG 
TCCCGGATGT TGGAGGACGG CTACGAACAG CTCCGGCAGC TTTCCCAGCA TGCCATGAAG 
GGTGTGATCC GAGTGAAGTT TGTCAACGAC CTCGGGGTGG ACGAGGCTGG GATTGACCAG 
GATGGTGTCT TCAAGGAGTT TCTGGAAGAG ATCATCAAGA GGGTGTTTGA CCCCGCACTC 
AACCTGTTCA AGACAACCAG CGGGGATGAG CGGCTGTACC CATCACCCAC GTCCTACATT 
CACGAGAATT ACCTGCAGCT CTTCGAGTTT GTGGGGAAGA TGCTGGGAAA GGCAGTGTAT 
GAGGGGATCG TGGTAGATGT GCCCTTTGCC TCCTTCTTCC TGAGCCAGAT GCTCGGGCAC 
CACCACAGCG TCTTCTACAG CTCCGTGGAT GAGCTTCCCT CCCTGGACTC GGAGTTTTAT 
AAAAACCTCA CCTCCATCAA GCGCTACGAT GGAGACATCG CAGACCTCGG GCTGACGCTG 
TCTTACGATG AAGACGTCAT GGGTCAGCTC GTCTGCCACG AACTGGTTCC CGGAGGGAAG 
ACCATCCCGG TTACAGATGA GAACAAAATT AGCTACATCC ATCTGATGGC GCATTTCCGA 
ATGCACACTC AAATTAAAAA CCAGACAGCT GCTCTTATCA GTGGCTTCCG TTCCATCATC 
AAGCCCGAGT GGATCCGAAT GTTCTCTACC CCTGAGCTTC AGCGCCTCAT CTCCGGGGAC 
AACGCTGAGA TAGATCTGGA AGACTTAAAG AAGCACACAG TGTACTACGG TGGCTTTCAT 
GGGAGCCACA GGGTCATCAT CTGGCTCTGG GATATCCTGG CTTCCGACTT CACGCCTGAA 
GAAAGAGCCA TGTTCCTGAA GTTTGTGACC AGCTGCTCCC GGCCCCCGCT CCTAGGATTC 
GCCTACCTCA AGCCTCCGTT CTCCATCCGC TGTGTGGAAG TATCAGATGA CCAGGACACT 
GGGGATACCC TGGGCAGCGT CCTCAGGGGC TTCTTCACCA TCCGCAAGAG GGAGCCAGGG 
GGGCGTCTGC CCACATCTTC CACTTGCTTC AACCTGCTTA AGCTGCCCAA CTACAGCAAG 
AAGAGCGTAC TGCGAGAGAA ACTACGCTAT GCCATCAGCA TGAACACGGG CTTTGAGCTC 
TCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011246463.1
CDS	347..1972
Translation	MLMLFCDCSRHLITILDDIEVYEEQISFKLEELVTISSFLNSFVFKMIWDGIVENAKGETLELFQSVHGWLMVLYERDCRRRFAPEDHWLRRDLKPGVLFQELDKDRRRAQLVLQHIPHVVPHKNRVLLFRNMVIKEKEKLGLVETSSASPHVTHITIRRSRMLEDGYEQLRQLSQHAMKGVIRVKFVNDLGVDEAGIDQDGVFKEFLEEIIKRVFDPALNLFKTTSGDERLYPSPTSYIHENYLQLFEFVGKMLGKAVYEGIVVDVPFASFFLSQMLGHHHSVFYSSVDELPSLDSEFYKNLTSIKRYDGDIADLGLTLSYDEDVMGQLVCHELVPGGKTIPVTDENKISYIHLMAHFRMHTQIKNQTAALISGFRSIIKPEWIRMFSTPELQRLISGDNAEIDLEDLKKHTVYYGGFHGSHRVIIWLWDILASDFTPEERAMFLKFVTSCSRPPLLGFAYLKPPFSIRCVEVSDDQDTGDTLGSVLRGFFTIRKREPGGRLPTSSTCFNLLKLPNYSKKSVLREKLRYAISMNTGFELS

Target ORF information:

RefSeq Version	XM_011248161.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus ubiquitin protein ligase E3B (Ube3b), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011248161.1

ORF Insert Sequence:

ATGCTGATGC TCTTCTGTGA CTGTTCCCGG CACCTCATCA CCATCCTCGA TGACATTGAA 
GTTTATGAAG AACAGATCTC CTTCAAGCTG GAAGAGCTGG TCACCATCTC CTCGTTCCTC 
AATTCCTTCG TGTTTAAGAT GATCTGGGAC GGAATTGTAG AGAATGCCAA GGGTGAGACC 
TTGGAGCTGT TCCAGTCCGT CCATGGCTGG CTGATGGTGC TGTATGAGCG AGACTGCCGC 
CGGCGCTTTG CTCCGGAGGA CCACTGGCTA CGGAGGGACC TCAAGCCTGG TGTGCTCTTC 
CAGGAGCTCG ACAAGGACCG AAGGAGGGCT CAGCTGGTCC TGCAGCACAT CCCCCACGTC 
GTGCCTCACA AGAACAGAGT TCTTCTGTTC CGGAACATGG TTATCAAGGA GAAAGAGAAG 
CTGGGGCTTG TGGAGACCAG CTCTGCCTCT CCTCACGTCA CCCACATCAC CATCCGAAGG 
TCCCGGATGT TGGAGGACGG CTACGAACAG CTCCGGCAGC TTTCCCAGCA TGCCATGAAG 
GGTGTGATCC GAGTGAAGTT TGTCAACGAC CTCGGGGTGG ACGAGGCTGG GATTGACCAG 
GATGGTGTCT TCAAGGAGTT TCTGGAAGAG ATCATCAAGA GGGTGTTTGA CCCCGCACTC 
AACCTGTTCA AGACAACCAG CGGGGATGAG CGGCTGTACC CATCACCCAC GTCCTACATT 
CACGAGAATT ACCTGCAGCT CTTCGAGTTT GTGGGGAAGA TGCTGGGAAA GGCAGTGTAT 
GAGGGGATCG TGGTAGATGT GCCCTTTGCC TCCTTCTTCC TGAGCCAGAT GCTCGGGCAC 
CACCACAGCG TCTTCTACAG CTCCGTGGAT GAGCTTCCCT CCCTGGACTC GGAGTTTTAT 
AAAAACCTCA CCTCCATCAA GCGCTACGAT GGAGACATCG CAGACCTCGG GCTGACGCTG 
TCTTACGATG AAGACGTCAT GGGTCAGCTC GTCTGCCACG AACTGGTTCC CGGAGGGAAG 
ACCATCCCGG TTACAGATGA GAACAAAATT AGCTACATCC ATCTGATGGC GCATTTCCGA 
ATGCACACTC AAATTAAAAA CCAGACAGCT GCTCTTATCA GTGGCTTCCG TTCCATCATC 
AAGCCCGAGT GGATCCGAAT GTTCTCTACC CCTGAGCTTC AGCGCCTCAT CTCCGGGGAC 
AACGCTGAGA TAGATCTGGA AGACTTAAAG AAGCACACAG TGTACTACGG TGGCTTTCAT 
GGGAGCCACA GGGTCATCAT CTGGCTCTGG GATATCCTGG CTTCCGACTT CACGCCTGAA 
GAAAGAGCCA TGTTCCTGAA GTTTGTGACC AGCTGCTCCC GGCCCCCGCT CCTAGGATTC 
GCCTACCTCA AGCCTCCGTT CTCCATCCGC TGTGTGGAAG TATCAGATGA CCAGGACACT 
GGGGATACCC TGGGCAGCGT CCTCAGGGGC TTCTTCACCA TCCGCAAGAG GGAGCCAGGG 
GGGCGTCTGC CCACATCTTC CACTTGCTTC AACCTGCTTA AGCTGCCCAA CTACAGCAAG 
AAGAGCGTAC TGCGAGAGAA ACTACGCTAT GCCATCAGCA TGAACACGGG CTTTGAGCTC 
TCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

UBE3B ( NM_001331219.1 ) cDNA ORF clone, Mus musculus(House mouse) -> NP_001318148.1 Mus musculus ubiquitin protein ligase E3B (Ube3b), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OMu83388
Clone ID Related Accession (Same CDS sequence)	NM_001331219.1
Accession Version	NM_001331219.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2532bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-24
Organism	Mus musculus(house mouse)
Product	ubiquitin-protein ligase E3B isoform 2 precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC132102.3. Transcript Variant: This variant (2) lacks an exon in the 5' region and initiates translation at a downstream start codon compared to variant 1. The encoded isoform (2) has a shorter N-terminus than isoform 1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: AK088518.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTTAACAG CAGCCTTCTC TCTGGCCCTG CGCCCTGTGG TTGCTGCGCA GTTCTCAGAC 
AATCTTATGC GACCATTCAT CATCCACGTC ATGTCGGTAC CTGCTCTCGT GGCTCATCTC 
AGCACCGTGG CCCCTGAGCG CCTCGGCGTT TTAGAGTCCC ATGACATGCT TCGTAAATTC 
ATCGTCTTCC TGAGAGATAG AGACCGATGC CGCGATGCTT GTGAGAGTTT AGAAGGCTGC 
CATACCCTGT GTCTCATGGG CAATCTCCTG CACTTGGGCT CCCTCAGCCT CAGGCTGTTA 
GAGGAAGAGA TGGATGGGTT TGTGAGCGCG CTCACGCAGA TGCTGTGCTA CTGTCAGAAG 
TACGTGGCTC AGAAGAAGTC CAACCTGACC CACTGGCACC CTGTGCTCGG CTGGTTTTCC 
CAACCTGTGG ATTACGGCCT CAATGATTCC ATGTACCTGA TCACCAAGCA GCTGCAGTTC 
CTGTGGGCGG TGCCTCTCAT CCGCATTCTC TTCAGTGACA TCCTGAGCAG GAAGCTGCTG 
GAGCATGCAG AGCCGGCCCC GGTGCAACCG CAGCCGTCCT CACCGCAGAC GGTGCTCCCC 
GTGAAGAGCC TCCTCAAGCG TGCGTTTCAG AAGTCGGCTT CGGTTCGGAA CATCCTCAGG 
CCTGTTGGGG GCCGCCGTGT CGACTCCGCT GAGGTGCGGA AGGTCTGCAA CATTTGCGTG 
CTCTACCAGA CCTCGCTGAC GACCCTCACC CAGATCCGCC TGCAGATCCT CACAGGTCTT 
ACGTACCTGG ATGACCTGCT GCCCAAGCTC TGGGCGTTTA TCTGTGAGCT GGGGCCCCAC 
GGCGGGCTCA AGCTCTTCCT GGAGTGCCTG AACAATGACA CTGGAGAGTC CAAGCAGCTC 
CTGGCCATGC TGATGCTCTT CTGTGACTGT TCCCGGCACC TCATCACCAT CCTCGATGAC 
ATTGAAGTTT ATGAAGAACA GATCTCCTTC AAGCTGGAAG AGCTGGTCAC CATCTCCTCG 
TTCCTCAATT CCTTCGTGTT TAAGATGATC TGGGACGGAA TTGTAGAGAA TGCCAAGGGT 
GAGACCTTGG AGCTGTTCCA GTCCGTCCAT GGCTGGCTGA TGGTGCTGTA TGAGCGAGAC 
TGCCGCCGGC GCTTTGCTCC GGAGGACCAC TGGCTACGGA GGGACCTCAA GCCTGGTGTG 
CTCTTCCAGG AGCTCGACAA GGACCGAAGG AGGGCTCAGC TGGTCCTGCA GCACATCCCC 
CACGTCGTGC CTCACAAGAA CAGAGTTCTT CTGTTCCGGA ACATGGTTAT CAAGGAGAAA 
GAGAAGCTGG GGCTTGTGGA GACCAGCTCT GCCTCTCCTC ACGTCACCCA CATCACCATC 
CGAAGGTCCC GGATGTTGGA GGACGGCTAC GAACAGCTCC GGCAGCTTTC CCAGCATGCC 
ATGAAGGGTG TGATCCGAGT GAAGTTTGTC AACGACCTCG GGGTGGACGA GGCTGGGATT 
GACCAGGATG GTGTCTTCAA GGAGTTTCTG GAAGAGATCA TCAAGAGGGT GTTTGACCCC 
GCACTCAACC TGTTCAAGAC AACCAGCGGG GATGAGCGGC TGTACCCATC ACCCACGTCC 
TACATTCACG AGAATTACCT GCAGCTCTTC GAGTTTGTGG GGAAGATGCT GGGAAAGGCA 
GTGTATGAGG GGATCGTGGT AGATGTGCCC TTTGCCTCCT TCTTCCTGAG CCAGATGCTC 
GGGCACCACC ACAGCGTCTT CTACAGCTCC GTGGATGAGC TTCCCTCCCT GGACTCGGAG 
TTTTATAAAA ACCTCACCTC CATCAAGCGC TACGATGGAG ACATCGCAGA CCTCGGGCTG 
ACGCTGTCTT ACGATGAAGA CGTCATGGGT CAGCTCGTCT GCCACGAACT GGTTCCCGGA 
GGGAAGACCA TCCCGGTTAC AGATGAGAAC AAAATTAGCT ACATCCATCT GATGGCGCAT 
TTCCGAATGC ACACTCAAAT TAAAAACCAG ACAGCTGCTC TTATCAGTGG CTTCCGTTCC 
ATCATCAAGC CCGAGTGGAT CCGAATGTTC TCTACCCCTG AGCTTCAGCG CCTCATCTCC 
GGGGACAACG CTGAGATAGA TCTGGAAGAC TTAAAGAAGC ACACAGTGTA CTACGGTGGC 
TTTCATGGGA GCCACAGGGT CATCATCTGG CTCTGGGATA TCCTGGCTTC CGACTTCACG 
CCTGAAGAAA GAGCCATGTT CCTGAAGTTT GTGACCAGCT GCTCCCGGCC CCCGCTCCTA 
GGATTCGCCT ACCTCAAGCC TCCGTTCTCC ATCCGCTGTG TGGAAGTATC AGATGACCAG 
GACACTGGGG ATACCCTGGG CAGCGTCCTC AGGGGCTTCT TCACCATCCG CAAGAGGGAG 
CCAGGGGGGC GTCTGCCCAC ATCTTCCACT TGCTTCAACC TGCTTAAGCT GCCCAACTAC 
AGCAAGAAGA GCGTACTGCG AGAGAAACTA CGCTATGCCA TCAGCATGAA CACGGGCTTT 
GAGCTCTCCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001318148.1
CDS	1064..3595
Translation	MLTAAFSLALRPVVAAQFSDNLMRPFIIHVMSVPALVAHLSTVAPERLGVLESHDMLRKFIVFLRDRDRCRDACESLEGCHTLCLMGNLLHLGSLSLRLLEEEMDGFVSALTQMLCYCQKYVAQKKSNLTHWHPVLGWFSQPVDYGLNDSMYLITKQLQFLWAVPLIRILFSDILSRKLLEHAEPAPVQPQPSSPQTVLPVKSLLKRAFQKSASVRNILRPVGGRRVDSAEVRKVCNICVLYQTSLTTLTQIRLQILTGLTYLDDLLPKLWAFICELGPHGGLKLFLECLNNDTGESKQLLAMLMLFCDCSRHLITILDDIEVYEEQISFKLEELVTISSFLNSFVFKMIWDGIVENAKGETLELFQSVHGWLMVLYERDCRRRFAPEDHWLRRDLKPGVLFQELDKDRRRAQLVLQHIPHVVPHKNRVLLFRNMVIKEKEKLGLVETSSASPHVTHITIRRSRMLEDGYEQLRQLSQHAMKGVIRVKFVNDLGVDEAGIDQDGVFKEFLEEIIKRVFDPALNLFKTTSGDERLYPSPTSYIHENYLQLFEFVGKMLGKAVYEGIVVDVPFASFFLSQMLGHHHSVFYSSVDELPSLDSEFYKNLTSIKRYDGDIADLGLTLSYDEDVMGQLVCHELVPGGKTIPVTDENKISYIHLMAHFRMHTQIKNQTAALISGFRSIIKPEWIRMFSTPELQRLISGDNAEIDLEDLKKHTVYYGGFHGSHRVIIWLWDILASDFTPEERAMFLKFVTSCSRPPLLGFAYLKPPFSIRCVEVSDDQDTGDTLGSVLRGFFTIRKREPGGRLPTSSTCFNLLKLPNYSKKSVLREKLRYAISMNTGFELS

Target ORF information:

RefSeq Version	NM_001331219.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus ubiquitin protein ligase E3B (Ube3b), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001331219.1

ORF Insert Sequence:

ATGTTAACAG CAGCCTTCTC TCTGGCCCTG CGCCCTGTGG TTGCTGCGCA GTTCTCAGAC 
AATCTTATGC GACCATTCAT CATCCACGTC ATGTCGGTAC CTGCTCTCGT GGCTCATCTC 
AGCACCGTGG CCCCTGAGCG CCTCGGCGTT TTAGAGTCCC ATGACATGCT TCGTAAATTC 
ATCGTCTTCC TGAGAGATAG AGACCGATGC CGCGATGCTT GTGAGAGTTT AGAAGGCTGC 
CATACCCTGT GTCTCATGGG CAATCTCCTG CACTTGGGCT CCCTCAGCCT CAGGCTGTTA 
GAGGAAGAGA TGGATGGGTT TGTGAGCGCG CTCACGCAGA TGCTGTGCTA CTGTCAGAAG 
TACGTGGCTC AGAAGAAGTC CAACCTGACC CACTGGCACC CTGTGCTCGG CTGGTTTTCC 
CAACCTGTGG ATTACGGCCT CAATGATTCC ATGTACCTGA TCACCAAGCA GCTGCAGTTC 
CTGTGGGCGG TGCCTCTCAT CCGCATTCTC TTCAGTGACA TCCTGAGCAG GAAGCTGCTG 
GAGCATGCAG AGCCGGCCCC GGTGCAACCG CAGCCGTCCT CACCGCAGAC GGTGCTCCCC 
GTGAAGAGCC TCCTCAAGCG TGCGTTTCAG AAGTCGGCTT CGGTTCGGAA CATCCTCAGG 
CCTGTTGGGG GCCGCCGTGT CGACTCCGCT GAGGTGCGGA AGGTCTGCAA CATTTGCGTG 
CTCTACCAGA CCTCGCTGAC GACCCTCACC CAGATCCGCC TGCAGATCCT CACAGGTCTT 
ACGTACCTGG ATGACCTGCT GCCCAAGCTC TGGGCGTTTA TCTGTGAGCT GGGGCCCCAC 
GGCGGGCTCA AGCTCTTCCT GGAGTGCCTG AACAATGACA CTGGAGAGTC CAAGCAGCTC 
CTGGCCATGC TGATGCTCTT CTGTGACTGT TCCCGGCACC TCATCACCAT CCTCGATGAC 
ATTGAAGTTT ATGAAGAACA GATCTCCTTC AAGCTGGAAG AGCTGGTCAC CATCTCCTCG 
TTCCTCAATT CCTTCGTGTT TAAGATGATC TGGGACGGAA TTGTAGAGAA TGCCAAGGGT 
GAGACCTTGG AGCTGTTCCA GTCCGTCCAT GGCTGGCTGA TGGTGCTGTA TGAGCGAGAC 
TGCCGCCGGC GCTTTGCTCC GGAGGACCAC TGGCTACGGA GGGACCTCAA GCCTGGTGTG 
CTCTTCCAGG AGCTCGACAA GGACCGAAGG AGGGCTCAGC TGGTCCTGCA GCACATCCCC 
CACGTCGTGC CTCACAAGAA CAGAGTTCTT CTGTTCCGGA ACATGGTTAT CAAGGAGAAA 
GAGAAGCTGG GGCTTGTGGA GACCAGCTCT GCCTCTCCTC ACGTCACCCA CATCACCATC 
CGAAGGTCCC GGATGTTGGA GGACGGCTAC GAACAGCTCC GGCAGCTTTC CCAGCATGCC 
ATGAAGGGTG TGATCCGAGT GAAGTTTGTC AACGACCTCG GGGTGGACGA GGCTGGGATT 
GACCAGGATG GTGTCTTCAA GGAGTTTCTG GAAGAGATCA TCAAGAGGGT GTTTGACCCC 
GCACTCAACC TGTTCAAGAC AACCAGCGGG GATGAGCGGC TGTACCCATC ACCCACGTCC 
TACATTCACG AGAATTACCT GCAGCTCTTC GAGTTTGTGG GGAAGATGCT GGGAAAGGCA 
GTGTATGAGG GGATCGTGGT AGATGTGCCC TTTGCCTCCT TCTTCCTGAG CCAGATGCTC 
GGGCACCACC ACAGCGTCTT CTACAGCTCC GTGGATGAGC TTCCCTCCCT GGACTCGGAG 
TTTTATAAAA ACCTCACCTC CATCAAGCGC TACGATGGAG ACATCGCAGA CCTCGGGCTG 
ACGCTGTCTT ACGATGAAGA CGTCATGGGT CAGCTCGTCT GCCACGAACT GGTTCCCGGA 
GGGAAGACCA TCCCGGTTAC AGATGAGAAC AAAATTAGCT ACATCCATCT GATGGCGCAT 
TTCCGAATGC ACACTCAAAT TAAAAACCAG ACAGCTGCTC TTATCAGTGG CTTCCGTTCC 
ATCATCAAGC CCGAGTGGAT CCGAATGTTC TCTACCCCTG AGCTTCAGCG CCTCATCTCC 
GGGGACAACG CTGAGATAGA TCTGGAAGAC TTAAAGAAGC ACACAGTGTA CTACGGTGGC 
TTTCATGGGA GCCACAGGGT CATCATCTGG CTCTGGGATA TCCTGGCTTC CGACTTCACG 
CCTGAAGAAA GAGCCATGTT CCTGAAGTTT GTGACCAGCT GCTCCCGGCC CCCGCTCCTA 
GGATTCGCCT ACCTCAAGCC TCCGTTCTCC ATCCGCTGTG TGGAAGTATC AGATGACCAG 
GACACTGGGG ATACCCTGGG CAGCGTCCTC AGGGGCTTCT TCACCATCCG CAAGAGGGAG 
CCAGGGGGGC GTCTGCCCAC ATCTTCCACT TGCTTCAACC TGCTTAAGCT GCCCAACTAC 
AGCAAGAAGA GCGTACTGCG AGAGAAACTA CGCTATGCCA TCAGCATGAA CACGGGCTTT 
GAGCTCTCCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

UBE3B ( XM_011248161.2 ) cDNA ORF clone, Mus musculus(House mouse) -> XP_011246463.1 Mus musculus ubiquitin protein ligase E3B (Ube3b), transcript variant X3, mRNA.

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CloneID	OMu72445
Clone ID Related Accession (Same CDS sequence)	XM_011248161.2 , XM_011248161.1
Accession Version	XM_011248161.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1626bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	ubiquitin-protein ligase E3B isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000071.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_011248161.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGATGC TCTTCTGTGA CTGTTCCCGG CACCTCATCA CCATCCTCGA TGACATTGAA 
GTTTATGAAG AACAGATCTC CTTCAAGCTG GAAGAGCTGG TCACCATCTC CTCGTTCCTC 
AATTCCTTCG TGTTTAAGAT GATCTGGGAC GGAATTGTAG AGAATGCCAA GGGTGAGACC 
TTGGAGCTGT TCCAGTCCGT CCATGGCTGG CTGATGGTGC TGTATGAGCG AGACTGCCGC 
CGGCGCTTTG CTCCGGAGGA CCACTGGCTA CGGAGGGACC TCAAGCCTGG TGTGCTCTTC 
CAGGAGCTCG ACAAGGACCG AAGGAGGGCT CAGCTGGTCC TGCAGCACAT CCCCCACGTC 
GTGCCTCACA AGAACAGAGT TCTTCTGTTC CGGAACATGG TTATCAAGGA GAAAGAGAAG 
CTGGGGCTTG TGGAGACCAG CTCTGCCTCT CCTCACGTCA CCCACATCAC CATCCGAAGG 
TCCCGGATGT TGGAGGACGG CTACGAACAG CTCCGGCAGC TTTCCCAGCA TGCCATGAAG 
GGTGTGATCC GAGTGAAGTT TGTCAACGAC CTCGGGGTGG ACGAGGCTGG GATTGACCAG 
GATGGTGTCT TCAAGGAGTT TCTGGAAGAG ATCATCAAGA GGGTGTTTGA CCCCGCACTC 
AACCTGTTCA AGACAACCAG CGGGGATGAG CGGCTGTACC CATCACCCAC GTCCTACATT 
CACGAGAATT ACCTGCAGCT CTTCGAGTTT GTGGGGAAGA TGCTGGGAAA GGCAGTGTAT 
GAGGGGATCG TGGTAGATGT GCCCTTTGCC TCCTTCTTCC TGAGCCAGAT GCTCGGGCAC 
CACCACAGCG TCTTCTACAG CTCCGTGGAT GAGCTTCCCT CCCTGGACTC GGAGTTTTAT 
AAAAACCTCA CCTCCATCAA GCGCTACGAT GGAGACATCG CAGACCTCGG GCTGACGCTG 
TCTTACGATG AAGACGTCAT GGGTCAGCTC GTCTGCCACG AACTGGTTCC CGGAGGGAAG 
ACCATCCCGG TTACAGATGA GAACAAAATT AGCTACATCC ATCTGATGGC GCATTTCCGA 
ATGCACACTC AAATTAAAAA CCAGACAGCT GCTCTTATCA GTGGCTTCCG TTCCATCATC 
AAGCCCGAGT GGATCCGAAT GTTCTCTACC CCTGAGCTTC AGCGCCTCAT CTCCGGGGAC 
AACGCTGAGA TAGATCTGGA AGACTTAAAG AAGCACACAG TGTACTACGG TGGCTTTCAT 
GGGAGCCACA GGGTCATCAT CTGGCTCTGG GATATCCTGG CTTCCGACTT CACGCCTGAA 
GAAAGAGCCA TGTTCCTGAA GTTTGTGACC AGCTGCTCCC GGCCCCCGCT CCTAGGATTC 
GCCTACCTCA AGCCTCCGTT CTCCATCCGC TGTGTGGAAG TATCAGATGA CCAGGACACT 
GGGGATACCC TGGGCAGCGT CCTCAGGGGC TTCTTCACCA TCCGCAAGAG GGAGCCAGGG 
GGGCGTCTGC CCACATCTTC CACTTGCTTC AACCTGCTTA AGCTGCCCAA CTACAGCAAG 
AAGAGCGTAC TGCGAGAGAA ACTACGCTAT GCCATCAGCA TGAACACGGG CTTTGAGCTC 
TCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011246463.1
CDS	1310..2935
Translation	MLMLFCDCSRHLITILDDIEVYEEQISFKLEELVTISSFLNSFVFKMIWDGIVENAKGETLELFQSVHGWLMVLYERDCRRRFAPEDHWLRRDLKPGVLFQELDKDRRRAQLVLQHIPHVVPHKNRVLLFRNMVIKEKEKLGLVETSSASPHVTHITIRRSRMLEDGYEQLRQLSQHAMKGVIRVKFVNDLGVDEAGIDQDGVFKEFLEEIIKRVFDPALNLFKTTSGDERLYPSPTSYIHENYLQLFEFVGKMLGKAVYEGIVVDVPFASFFLSQMLGHHHSVFYSSVDELPSLDSEFYKNLTSIKRYDGDIADLGLTLSYDEDVMGQLVCHELVPGGKTIPVTDENKISYIHLMAHFRMHTQIKNQTAALISGFRSIIKPEWIRMFSTPELQRLISGDNAEIDLEDLKKHTVYYGGFHGSHRVIIWLWDILASDFTPEERAMFLKFVTSCSRPPLLGFAYLKPPFSIRCVEVSDDQDTGDTLGSVLRGFFTIRKREPGGRLPTSSTCFNLLKLPNYSKKSVLREKLRYAISMNTGFELS

Target ORF information:

RefSeq Version	XM_011248161.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus ubiquitin protein ligase E3B (Ube3b), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011248161.2

ORF Insert Sequence:

ATGCTGATGC TCTTCTGTGA CTGTTCCCGG CACCTCATCA CCATCCTCGA TGACATTGAA 
GTTTATGAAG AACAGATCTC CTTCAAGCTG GAAGAGCTGG TCACCATCTC CTCGTTCCTC 
AATTCCTTCG TGTTTAAGAT GATCTGGGAC GGAATTGTAG AGAATGCCAA GGGTGAGACC 
TTGGAGCTGT TCCAGTCCGT CCATGGCTGG CTGATGGTGC TGTATGAGCG AGACTGCCGC 
CGGCGCTTTG CTCCGGAGGA CCACTGGCTA CGGAGGGACC TCAAGCCTGG TGTGCTCTTC 
CAGGAGCTCG ACAAGGACCG AAGGAGGGCT CAGCTGGTCC TGCAGCACAT CCCCCACGTC 
GTGCCTCACA AGAACAGAGT TCTTCTGTTC CGGAACATGG TTATCAAGGA GAAAGAGAAG 
CTGGGGCTTG TGGAGACCAG CTCTGCCTCT CCTCACGTCA CCCACATCAC CATCCGAAGG 
TCCCGGATGT TGGAGGACGG CTACGAACAG CTCCGGCAGC TTTCCCAGCA TGCCATGAAG 
GGTGTGATCC GAGTGAAGTT TGTCAACGAC CTCGGGGTGG ACGAGGCTGG GATTGACCAG 
GATGGTGTCT TCAAGGAGTT TCTGGAAGAG ATCATCAAGA GGGTGTTTGA CCCCGCACTC 
AACCTGTTCA AGACAACCAG CGGGGATGAG CGGCTGTACC CATCACCCAC GTCCTACATT 
CACGAGAATT ACCTGCAGCT CTTCGAGTTT GTGGGGAAGA TGCTGGGAAA GGCAGTGTAT 
GAGGGGATCG TGGTAGATGT GCCCTTTGCC TCCTTCTTCC TGAGCCAGAT GCTCGGGCAC 
CACCACAGCG TCTTCTACAG CTCCGTGGAT GAGCTTCCCT CCCTGGACTC GGAGTTTTAT 
AAAAACCTCA CCTCCATCAA GCGCTACGAT GGAGACATCG CAGACCTCGG GCTGACGCTG 
TCTTACGATG AAGACGTCAT GGGTCAGCTC GTCTGCCACG AACTGGTTCC CGGAGGGAAG 
ACCATCCCGG TTACAGATGA GAACAAAATT AGCTACATCC ATCTGATGGC GCATTTCCGA 
ATGCACACTC AAATTAAAAA CCAGACAGCT GCTCTTATCA GTGGCTTCCG TTCCATCATC 
AAGCCCGAGT GGATCCGAAT GTTCTCTACC CCTGAGCTTC AGCGCCTCAT CTCCGGGGAC 
AACGCTGAGA TAGATCTGGA AGACTTAAAG AAGCACACAG TGTACTACGG TGGCTTTCAT 
GGGAGCCACA GGGTCATCAT CTGGCTCTGG GATATCCTGG CTTCCGACTT CACGCCTGAA 
GAAAGAGCCA TGTTCCTGAA GTTTGTGACC AGCTGCTCCC GGCCCCCGCT CCTAGGATTC 
GCCTACCTCA AGCCTCCGTT CTCCATCCGC TGTGTGGAAG TATCAGATGA CCAGGACACT 
GGGGATACCC TGGGCAGCGT CCTCAGGGGC TTCTTCACCA TCCGCAAGAG GGAGCCAGGG 
GGGCGTCTGC CCACATCTTC CACTTGCTTC AACCTGCTTA AGCTGCCCAA CTACAGCAAG 
AAGAGCGTAC TGCGAGAGAA ACTACGCTAT GCCATCAGCA TGAACACGGG CTTTGAGCTC 
TCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

UBE3B ( NM_054093.2 ) cDNA ORF clone, Mus musculus(House mouse) -> NP_473434.2 Mus musculus ubiquitin protein ligase E3B (Ube3b), mRNA.

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CloneID	OMu09583
Clone ID Related Accession (Same CDS sequence)	NM_054093.2 , NM_054093.3 , XM_006530125.1
Accession Version	NM_054093.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3213bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-02-14
Organism	Mus musculus(House mouse)
Product	ubiquitin-protein ligase E3B
Comment

ATGTTCACTG TATCTCAGAC CTCCAGAGCA TGGTTCATCG ACAGAGCCCG GCAGGCACGA 
GAAGAGAGGC TGGTGCAGAA GGAGCGGGAG CGCTCAGCGG TGACGATCCA GGCCCTAGTC 
CGCAGTTTCC TCTGCCGGAG GCGACTGCAC AGAGACATCA GGAAAGAGAT TGATGAGTTT 
TTTAGTGCTG ATGAGTCTGG GTCCAGTAAA AGAAGTGCAC TTTGTATTTT TAAGATTGCC 
AGGAGACTGC TGTTTATATG CAAAACTACC GAGGACAGCG AGAGACTGGA GAAGTTGTGT 
CGAAGCATCC TGAACAGCAT GGATGCCGAG AATGAGCCAA AGGTGTGGTA CGTGTCCCTG 
GCTCTCTCCA AGGACCTCAC GCTGCTGTGG ATTAAGCAGA TCAAGAGCAT CCTGTGGCAC 
TGCTGTGAGC TTCTGGGGCA GCTCAAGCCA GAGATCTTAC AGGACTCCAG GCTCATCACG 
CTGTACCTCA CCATGCTTGT GACCTTCACA GACACTTCAA CATGGAAGAT TCTCCGGGGA 
AAAGGTGAGA GCCTTCGGCC AGCCCTGAAC CACATTTGCG CAAATATAAT GGGCCATCTC 
AACCAGCGTG GACTTTACTC TGTATTGCAG GTACTGCTGA CCCGAGGCCT GGCCAGGCCC 
CGGCCCTGTC TGTCCAAAGG CATGTTAACA GCAGCCTTCT CTCTGGCCCT GCGCCCTGTG 
GTTGCTGCGC AGTTCTCAGA CAATCTTATG CGACCATTCA TCATCCACGT CATGTCGGTA 
CCTGCTCTCG TGGCTCATCT CAGCACCGTG GCCCCTGAGC GCCTCGGCGT TTTAGAGTCC 
CATGACATGC TTCGTAAATT CATCGTCTTC CTGAGAGATA GAGACCGATG CCGCGATGCT 
TGTGAGAGTT TAGAAGGCTG CCATACCCTG TGTCTCATGG GCAATCTCCT GCACTTGGGC 
TCCCTCAGCC TCAGGCTGTT AGAGGAAGAG ATGGATGGGT TTGTGAGCGC GCTCACGCAG 
ATGCTGTGCT ACTGTCAGAA GTACGTGGCT CAGAAGAAGT CCAACCTGAC CCACTGGCAC 
CCTGTGCTCG GCTGGTTTTC CCAACCTGTG GATTACGGCC TCAATGATTC CATGTACCTG 
ATCACCAAGC AGCTGCAGTT CCTGTGGGCG GTGCCTCTCA TCCGCATTCT CTTCAGTGAC 
ATCCTGAGCA GGAAGCTGCT GGAGCATGCA GAGCCGGCCC CGGTGCAACC GCAGCCGTCC 
TCACCGCAGA CGGTGCTCCC CGTGAAGAGC CTCCTCAAGC GTGCGTTTCA GAAGTCGGCT 
TCGGTTCGGA ACATCCTCAG GCCTGTTGGG GGCCGCCGTG TCGACTCCGC TGAGGTGCGG 
AAGGTCTGCA ACATTTGCGT GCTCTACCAG ACCTCGCTGA CGACCCTCAC CCAGATCCGC 
CTGCAGATCC TCACAGGTCT TACGTACCTG GATGACCTGC TGCCCAAGCT CTGGGCGTTT 
ATCTGTGAGC TGGGGCCCCA CGGCGGGCTC AAGCTCTTCC TGGAGTGCCT GAACAATGAC 
ACTGGAGAGT CCAAGCAGCT CCTGGCCATG CTGATGCTCT TCTGTGACTG TTCCCGGCAC 
CTCATCACCA TCCTCGATGA CATTGAAGTT TATGAAGAAC AGATCTCCTT CAAGCTGGAA 
GAGCTGGTCA CCATCTCCTC GTTCCTCAAT TCCTTCGTGT TTAAGATGAT CTGGGACGGA 
ATTGTAGAGA ATGCCAAGGG TGAGACCTTG GAGCTGTTCC AGTCCGTCCA TGGCTGGCTG 
ATGGTGCTGT ATGAGCGAGA CTGCCGCCGG CGCTTTGCTC CGGAGGACCA CTGGCTACGG 
AGGGACCTCA AGCCTGGTGT GCTCTTCCAG GAGCTCGACA AGGACCGAAG GAGGGCTCAG 
CTGGTCCTGC AGCACATCCC CCACGTCGTG CCTCACAAGA ACAGAGTTCT TCTGTTCCGG 
AACATGGTTA TCAAGGAGAA AGAGAAGCTG GGGCTTGTGG AGACCAGCTC TGCCTCTCCT 
CACGTCACCC ACATCACCAT CCGAAGGTCC CGGATGTTGG AGGACGGCTA CGAACAGCTC 
CGGCAGCTTT CCCAGCATGC CATGAAGGGT GTGATCCGAG TGAAGTTTGT CAACGACCTC 
GGGGTGGACG AGGCTGGGAT TGACCAGGAT GGTGTCTTCA AGGAGTTTCT GGAAGAGATC 
ATCAAGAGGG TGTTTGACCC CGCACTCAAC CTGTTCAAGA CAACCAGCGG GGATGAGCGG 
CTGTACCCAT CACCCACGTC CTACATTCAC GAGAATTACC TGCAGCTCTT CGAGTTTGTG 
GGGAAGATGC TGGGAAAGGC AGTGTATGAG GGGATCGTGG TAGATGTGCC CTTTGCCTCC 
TTCTTCCTGA GCCAGATGCT CGGGCACCAC CACAGCGTCT TCTACAGCTC CGTGGATGAG 
CTTCCCTCCC TGGACTCGGA GTTTTATAAA AACCTCACCT CCATCAAGCG CTACGATGGA 
GACATCGCAG ACCTCGGGCT GACGCTGTCT TACGATGAAG ACGTCATGGG TCAGCTCGTC 
TGCCACGAAC TGGTTCCCGG AGGGAAGACC ATCCCGGTTA CAGATGAGAA CAAAATTAGC 
TACATCCATC TGATGGCGCA TTTCCGAATG CACACTCAAA TTAAAAACCA GACAGCTGCT 
CTTATCAGTG GCTTCCGTTC CATCATCAAG CCCGAGTGGA TCCGAATGTT CTCTACCCCT 
GAGCTTCAGC GCCTCATCTC CGGGGACAAC GCTGAGATAG ATCTGGAAGA CTTAAAGAAG 
CACACAGTGT ACTACGGTGG CTTTCATGGG AGCCACAGGG TCATCATCTG GCTCTGGGAT 
ATCCTGGCTT CCGACTTCAC GCCTGAAGAA AGAGCCATGT TCCTGAAGTT TGTGACCAGC 
TGCTCCCGGC CCCCGCTCCT AGGATTCGCC TACCTCAAGC CTCCGTTCTC CATCCGCTGT 
GTGGAAGTAT CAGATGACCA GGACACTGGG GATACCCTGG GCAGCGTCCT CAGGGGCTTC 
TTCACCATCC GCAAGAGGGA GCCAGGGGGG CGTCTGCCCA CATCTTCCAC TTGCTTCAAC 
CTGCTTAAGC TGCCCAACTA CAGCAAGAAG AGCGTACTGC GAGAGAAACT ACGCTATGCC 
ATCAGCATGA ACACGGGCTT TGAGCTCTCC TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq

NP_473434.2

Translation

MFTVSQTSRAWFIDRARQAREERLVQKERERSAVTIQALVRSFLCRRRLHRDIRKEIDEFFSADESGSSKRSALCIFKIARRLLFICKTTEDSERLEKLCRSILNSMDAENEPKVWYVSLALSKDLTLLWIKQIKSILWHCCELLGQLKPEILQDSRLITLYLTMLVTFTDTSTWKILRGKGESLRPALNHICANIMGHLNQRGLYSVLQVLLTRGLARPRPCLSKGMLTAAFSLALRPVVAAQFSDNLMRPFIIHVMSVPALVAHLSTVAPERLGVLESHDMLRKFIVFLRDRDRCRDACESLEGCHTLCLMGNLLHLGSLSLRLLEEEMDGFVSALTQMLCYCQKYVAQKKSNLTHWHPVLGWFSQPVDYGLNDSMYLITKQLQFLWAVPLIRILFSDILSRKLLEHAEPAPVQPQPSSPQTVLPVKSLLKRAFQKSASVRNILRPVGGRRVDSAEVRKVCNICVLYQTSLTTLTQIRLQILTGLTYLDDLLPKLWAFICELGPHGGLKLFLECLNNDTGESKQLLAMLMLFCDCSRHLITILDDIEVYEEQISFKLEELVTISSFLNSFVFKMIWDGIVENAKGETLELFQSVHGWLMVLYERDCRRRFAPEDHWLRRDLKPGVLFQELDKDRRRAQLVLQHIPHVVPHKNRVLLFRNMVIKEKEKLGLVETSSASPHVTHITIRRSRMLEDGYEQLRQLSQHAMKGVIRVKFVNDLGVDEAGIDQDGVFKEFLEEIIKRVFDPALNLFKTTSGDERLYPSPTSYIHENYLQLFEFVGKMLGKAVYEGIVVDVPFASFFLSQMLGHHHSVFYSSVDELPSLDSEFYKNLTSIKRYDGDIADLGLTLSYDEDVMGQLVCHELVPGGKTIPVTDENKISYIHLMAHFRMHTQIKNQTAALISGFRSIIKPEWIRMFSTPELQRLISGDNAEIDLEDLKKHTVYYGGFHGSHRVIIWLWDILASDFTPEERAMFLKFVTSCSRPPLLGFAYLKPPFSIRCVEVSDDQDTGDTLGSVLRGFFTIRKREPGGRLPTSSTCFNLLKLPNYSKKSVLREKLRYAISMNTGFELS

Target ORF information:

RefSeq Version	NM_054093.2
Organism	Mus musculus(House mouse)
Definition	Mus musculus ubiquitin protein ligase E3B (Ube3b), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_054093.2

ORF Insert Sequence:

ATGTTCACTG TATCTCAGAC CTCCAGAGCA TGGTTCATCG ACAGAGCCCG GCAGGCACGA 
GAAGAGAGGC TGGTGCAGAA GGAGCGGGAG CGCTCAGCGG TGACGATCCA GGCCCTAGTC 
CGCAGTTTCC TCTGCCGGAG GCGACTGCAC AGAGACATCA GGAAAGAGAT TGATGAGTTT 
TTTAGTGCTG ATGAGTCTGG GTCCAGTAAA AGAAGTGCAC TTTGTATTTT TAAGATTGCC 
AGGAGACTGC TGTTTATATG CAAAACTACC GAGGACAGCG AGAGACTGGA GAAGTTGTGT 
CGAAGCATCC TGAACAGCAT GGATGCCGAG AATGAGCCAA AGGTGTGGTA CGTGTCCCTG 
GCTCTCTCCA AGGACCTCAC GCTGCTGTGG ATTAAGCAGA TCAAGAGCAT CCTGTGGCAC 
TGCTGTGAGC TTCTGGGGCA GCTCAAGCCA GAGATCTTAC AGGACTCCAG GCTCATCACG 
CTGTACCTCA CCATGCTTGT GACCTTCACA GACACTTCAA CATGGAAGAT TCTCCGGGGA 
AAAGGTGAGA GCCTTCGGCC AGCCCTGAAC CACATTTGCG CAAATATAAT GGGCCATCTC 
AACCAGCGTG GACTTTACTC TGTATTGCAG GTACTGCTGA CCCGAGGCCT GGCCAGGCCC 
CGGCCCTGTC TGTCCAAAGG CATGTTAACA GCAGCCTTCT CTCTGGCCCT GCGCCCTGTG 
GTTGCTGCGC AGTTCTCAGA CAATCTTATG CGACCATTCA TCATCCACGT CATGTCGGTA 
CCTGCTCTCG TGGCTCATCT CAGCACCGTG GCCCCTGAGC GCCTCGGCGT TTTAGAGTCC 
CATGACATGC TTCGTAAATT CATCGTCTTC CTGAGAGATA GAGACCGATG CCGCGATGCT 
TGTGAGAGTT TAGAAGGCTG CCATACCCTG TGTCTCATGG GCAATCTCCT GCACTTGGGC 
TCCCTCAGCC TCAGGCTGTT AGAGGAAGAG ATGGATGGGT TTGTGAGCGC GCTCACGCAG 
ATGCTGTGCT ACTGTCAGAA GTACGTGGCT CAGAAGAAGT CCAACCTGAC CCACTGGCAC 
CCTGTGCTCG GCTGGTTTTC CCAACCTGTG GATTACGGCC TCAATGATTC CATGTACCTG 
ATCACCAAGC AGCTGCAGTT CCTGTGGGCG GTGCCTCTCA TCCGCATTCT CTTCAGTGAC 
ATCCTGAGCA GGAAGCTGCT GGAGCATGCA GAGCCGGCCC CGGTGCAACC GCAGCCGTCC 
TCACCGCAGA CGGTGCTCCC CGTGAAGAGC CTCCTCAAGC GTGCGTTTCA GAAGTCGGCT 
TCGGTTCGGA ACATCCTCAG GCCTGTTGGG GGCCGCCGTG TCGACTCCGC TGAGGTGCGG 
AAGGTCTGCA ACATTTGCGT GCTCTACCAG ACCTCGCTGA CGACCCTCAC CCAGATCCGC 
CTGCAGATCC TCACAGGTCT TACGTACCTG GATGACCTGC TGCCCAAGCT CTGGGCGTTT 
ATCTGTGAGC TGGGGCCCCA CGGCGGGCTC AAGCTCTTCC TGGAGTGCCT GAACAATGAC 
ACTGGAGAGT CCAAGCAGCT CCTGGCCATG CTGATGCTCT TCTGTGACTG TTCCCGGCAC 
CTCATCACCA TCCTCGATGA CATTGAAGTT TATGAAGAAC AGATCTCCTT CAAGCTGGAA 
GAGCTGGTCA CCATCTCCTC GTTCCTCAAT TCCTTCGTGT TTAAGATGAT CTGGGACGGA 
ATTGTAGAGA ATGCCAAGGG TGAGACCTTG GAGCTGTTCC AGTCCGTCCA TGGCTGGCTG 
ATGGTGCTGT ATGAGCGAGA CTGCCGCCGG CGCTTTGCTC CGGAGGACCA CTGGCTACGG 
AGGGACCTCA AGCCTGGTGT GCTCTTCCAG GAGCTCGACA AGGACCGAAG GAGGGCTCAG 
CTGGTCCTGC AGCACATCCC CCACGTCGTG CCTCACAAGA ACAGAGTTCT TCTGTTCCGG 
AACATGGTTA TCAAGGAGAA AGAGAAGCTG GGGCTTGTGG AGACCAGCTC TGCCTCTCCT 
CACGTCACCC ACATCACCAT CCGAAGGTCC CGGATGTTGG AGGACGGCTA CGAACAGCTC 
CGGCAGCTTT CCCAGCATGC CATGAAGGGT GTGATCCGAG TGAAGTTTGT CAACGACCTC 
GGGGTGGACG AGGCTGGGAT TGACCAGGAT GGTGTCTTCA AGGAGTTTCT GGAAGAGATC 
ATCAAGAGGG TGTTTGACCC CGCACTCAAC CTGTTCAAGA CAACCAGCGG GGATGAGCGG 
CTGTACCCAT CACCCACGTC CTACATTCAC GAGAATTACC TGCAGCTCTT CGAGTTTGTG 
GGGAAGATGC TGGGAAAGGC AGTGTATGAG GGGATCGTGG TAGATGTGCC CTTTGCCTCC 
TTCTTCCTGA GCCAGATGCT CGGGCACCAC CACAGCGTCT TCTACAGCTC CGTGGATGAG 
CTTCCCTCCC TGGACTCGGA GTTTTATAAA AACCTCACCT CCATCAAGCG CTACGATGGA 
GACATCGCAG ACCTCGGGCT GACGCTGTCT TACGATGAAG ACGTCATGGG TCAGCTCGTC 
TGCCACGAAC TGGTTCCCGG AGGGAAGACC ATCCCGGTTA CAGATGAGAA CAAAATTAGC 
TACATCCATC TGATGGCGCA TTTCCGAATG CACACTCAAA TTAAAAACCA GACAGCTGCT 
CTTATCAGTG GCTTCCGTTC CATCATCAAG CCCGAGTGGA TCCGAATGTT CTCTACCCCT 
GAGCTTCAGC GCCTCATCTC CGGGGACAAC GCTGAGATAG ATCTGGAAGA CTTAAAGAAG 
CACACAGTGT ACTACGGTGG CTTTCATGGG AGCCACAGGG TCATCATCTG GCTCTGGGAT 
ATCCTGGCTT CCGACTTCAC GCCTGAAGAA AGAGCCATGT TCCTGAAGTT TGTGACCAGC 
TGCTCCCGGC CCCCGCTCCT AGGATTCGCC TACCTCAAGC CTCCGTTCTC CATCCGCTGT 
GTGGAAGTAT CAGATGACCA GGACACTGGG GATACCCTGG GCAGCGTCCT CAGGGGCTTC 
TTCACCATCC GCAAGAGGGA GCCAGGGGGG CGTCTGCCCA CATCTTCCAC TTGCTTCAAC 
CTGCTTAAGC TGCCCAACTA CAGCAAGAAG AGCGTACTGC GAGAGAAACT ACGCTATGCC 
ATCAGCATGA ACACGGGCTT TGAGCTCTCC TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

UBE3B ( NM_054093.3 ) cDNA ORF clone, Mus musculus(House mouse) -> NP_473434.2 Mus musculus ubiquitin protein ligase E3B (Ube3b), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OMu09583
Clone ID Related Accession (Same CDS sequence)	NM_054093.2 , NM_054093.3 , XM_006530125.1
Accession Version	NM_054093.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3213bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-24
Organism	Mus musculus(house mouse)
Product	ubiquitin-protein ligase E3B isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC132102.3. On Sep 13, 2016 this sequence version replaced NM_054093.2. Transcript Variant: This variant (1) represents the longest transcript and encodes the longer isoform (1). Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: SRR1660813.59773.1, BC023956.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTTCACTG TATCTCAGAC CTCCAGAGCA TGGTTCATCG ACAGAGCCCG GCAGGCACGA 
GAAGAGAGGC TGGTGCAGAA GGAGCGGGAG CGCTCAGCGG TGACGATCCA GGCCCTAGTC 
CGCAGTTTCC TCTGCCGGAG GCGACTGCAC AGAGACATCA GGAAAGAGAT TGATGAGTTT 
TTTAGTGCTG ATGAGTCTGG GTCCAGTAAA AGAAGTGCAC TTTGTATTTT TAAGATTGCC 
AGGAGACTGC TGTTTATATG CAAAACTACC GAGGACAGCG AGAGACTGGA GAAGTTGTGT 
CGAAGCATCC TGAACAGCAT GGATGCCGAG AATGAGCCAA AGGTGTGGTA CGTGTCCCTG 
GCTCTCTCCA AGGACCTCAC GCTGCTGTGG ATTAAGCAGA TCAAGAGCAT CCTGTGGCAC 
TGCTGTGAGC TTCTGGGGCA GCTCAAGCCA GAGATCTTAC AGGACTCCAG GCTCATCACG 
CTGTACCTCA CCATGCTTGT GACCTTCACA GACACTTCAA CATGGAAGAT TCTCCGGGGA 
AAAGGTGAGA GCCTTCGGCC AGCCCTGAAC CACATTTGCG CAAATATAAT GGGCCATCTC 
AACCAGCGTG GACTTTACTC TGTATTGCAG GTACTGCTGA CCCGAGGCCT GGCCAGGCCC 
CGGCCCTGTC TGTCCAAAGG CATGTTAACA GCAGCCTTCT CTCTGGCCCT GCGCCCTGTG 
GTTGCTGCGC AGTTCTCAGA CAATCTTATG CGACCATTCA TCATCCACGT CATGTCGGTA 
CCTGCTCTCG TGGCTCATCT CAGCACCGTG GCCCCTGAGC GCCTCGGCGT TTTAGAGTCC 
CATGACATGC TTCGTAAATT CATCGTCTTC CTGAGAGATA GAGACCGATG CCGCGATGCT 
TGTGAGAGTT TAGAAGGCTG CCATACCCTG TGTCTCATGG GCAATCTCCT GCACTTGGGC 
TCCCTCAGCC TCAGGCTGTT AGAGGAAGAG ATGGATGGGT TTGTGAGCGC GCTCACGCAG 
ATGCTGTGCT ACTGTCAGAA GTACGTGGCT CAGAAGAAGT CCAACCTGAC CCACTGGCAC 
CCTGTGCTCG GCTGGTTTTC CCAACCTGTG GATTACGGCC TCAATGATTC CATGTACCTG 
ATCACCAAGC AGCTGCAGTT CCTGTGGGCG GTGCCTCTCA TCCGCATTCT CTTCAGTGAC 
ATCCTGAGCA GGAAGCTGCT GGAGCATGCA GAGCCGGCCC CGGTGCAACC GCAGCCGTCC 
TCACCGCAGA CGGTGCTCCC CGTGAAGAGC CTCCTCAAGC GTGCGTTTCA GAAGTCGGCT 
TCGGTTCGGA ACATCCTCAG GCCTGTTGGG GGCCGCCGTG TCGACTCCGC TGAGGTGCGG 
AAGGTCTGCA ACATTTGCGT GCTCTACCAG ACCTCGCTGA CGACCCTCAC CCAGATCCGC 
CTGCAGATCC TCACAGGTCT TACGTACCTG GATGACCTGC TGCCCAAGCT CTGGGCGTTT 
ATCTGTGAGC TGGGGCCCCA CGGCGGGCTC AAGCTCTTCC TGGAGTGCCT GAACAATGAC 
ACTGGAGAGT CCAAGCAGCT CCTGGCCATG CTGATGCTCT TCTGTGACTG TTCCCGGCAC 
CTCATCACCA TCCTCGATGA CATTGAAGTT TATGAAGAAC AGATCTCCTT CAAGCTGGAA 
GAGCTGGTCA CCATCTCCTC GTTCCTCAAT TCCTTCGTGT TTAAGATGAT CTGGGACGGA 
ATTGTAGAGA ATGCCAAGGG TGAGACCTTG GAGCTGTTCC AGTCCGTCCA TGGCTGGCTG 
ATGGTGCTGT ATGAGCGAGA CTGCCGCCGG CGCTTTGCTC CGGAGGACCA CTGGCTACGG 
AGGGACCTCA AGCCTGGTGT GCTCTTCCAG GAGCTCGACA AGGACCGAAG GAGGGCTCAG 
CTGGTCCTGC AGCACATCCC CCACGTCGTG CCTCACAAGA ACAGAGTTCT TCTGTTCCGG 
AACATGGTTA TCAAGGAGAA AGAGAAGCTG GGGCTTGTGG AGACCAGCTC TGCCTCTCCT 
CACGTCACCC ACATCACCAT CCGAAGGTCC CGGATGTTGG AGGACGGCTA CGAACAGCTC 
CGGCAGCTTT CCCAGCATGC CATGAAGGGT GTGATCCGAG TGAAGTTTGT CAACGACCTC 
GGGGTGGACG AGGCTGGGAT TGACCAGGAT GGTGTCTTCA AGGAGTTTCT GGAAGAGATC 
ATCAAGAGGG TGTTTGACCC CGCACTCAAC CTGTTCAAGA CAACCAGCGG GGATGAGCGG 
CTGTACCCAT CACCCACGTC CTACATTCAC GAGAATTACC TGCAGCTCTT CGAGTTTGTG 
GGGAAGATGC TGGGAAAGGC AGTGTATGAG GGGATCGTGG TAGATGTGCC CTTTGCCTCC 
TTCTTCCTGA GCCAGATGCT CGGGCACCAC CACAGCGTCT TCTACAGCTC CGTGGATGAG 
CTTCCCTCCC TGGACTCGGA GTTTTATAAA AACCTCACCT CCATCAAGCG CTACGATGGA 
GACATCGCAG ACCTCGGGCT GACGCTGTCT TACGATGAAG ACGTCATGGG TCAGCTCGTC 
TGCCACGAAC TGGTTCCCGG AGGGAAGACC ATCCCGGTTA CAGATGAGAA CAAAATTAGC 
TACATCCATC TGATGGCGCA TTTCCGAATG CACACTCAAA TTAAAAACCA GACAGCTGCT 
CTTATCAGTG GCTTCCGTTC CATCATCAAG CCCGAGTGGA TCCGAATGTT CTCTACCCCT 
GAGCTTCAGC GCCTCATCTC CGGGGACAAC GCTGAGATAG ATCTGGAAGA CTTAAAGAAG 
CACACAGTGT ACTACGGTGG CTTTCATGGG AGCCACAGGG TCATCATCTG GCTCTGGGAT 
ATCCTGGCTT CCGACTTCAC GCCTGAAGAA AGAGCCATGT TCCTGAAGTT TGTGACCAGC 
TGCTCCCGGC CCCCGCTCCT AGGATTCGCC TACCTCAAGC CTCCGTTCTC CATCCGCTGT 
GTGGAAGTAT CAGATGACCA GGACACTGGG GATACCCTGG GCAGCGTCCT CAGGGGCTTC 
TTCACCATCC GCAAGAGGGA GCCAGGGGGG CGTCTGCCCA CATCTTCCAC TTGCTTCAAC 
CTGCTTAAGC TGCCCAACTA CAGCAAGAAG AGCGTACTGC GAGAGAAACT ACGCTATGCC 
ATCAGCATGA ACACGGGCTT TGAGCTCTCC TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_473434.2
CDS	469..3681
Translation	MFTVSQTSRAWFIDRARQAREERLVQKERERSAVTIQALVRSFLCRRRLHRDIRKEIDEFFSADESGSSKRSALCIFKIARRLLFICKTTEDSERLEKLCRSILNSMDAENEPKVWYVSLALSKDLTLLWIKQIKSILWHCCELLGQLKPEILQDSRLITLYLTMLVTFTDTSTWKILRGKGESLRPALNHICANIMGHLNQRGLYSVLQVLLTRGLARPRPCLSKGMLTAAFSLALRPVVAAQFSDNLMRPFIIHVMSVPALVAHLSTVAPERLGVLESHDMLRKFIVFLRDRDRCRDACESLEGCHTLCLMGNLLHLGSLSLRLLEEEMDGFVSALTQMLCYCQKYVAQKKSNLTHWHPVLGWFSQPVDYGLNDSMYLITKQLQFLWAVPLIRILFSDILSRKLLEHAEPAPVQPQPSSPQTVLPVKSLLKRAFQKSASVRNILRPVGGRRVDSAEVRKVCNICVLYQTSLTTLTQIRLQILTGLTYLDDLLPKLWAFICELGPHGGLKLFLECLNNDTGESKQLLAMLMLFCDCSRHLITILDDIEVYEEQISFKLEELVTISSFLNSFVFKMIWDGIVENAKGETLELFQSVHGWLMVLYERDCRRRFAPEDHWLRRDLKPGVLFQELDKDRRRAQLVLQHIPHVVPHKNRVLLFRNMVIKEKEKLGLVETSSASPHVTHITIRRSRMLEDGYEQLRQLSQHAMKGVIRVKFVNDLGVDEAGIDQDGVFKEFLEEIIKRVFDPALNLFKTTSGDERLYPSPTSYIHENYLQLFEFVGKMLGKAVYEGIVVDVPFASFFLSQMLGHHHSVFYSSVDELPSLDSEFYKNLTSIKRYDGDIADLGLTLSYDEDVMGQLVCHELVPGGKTIPVTDENKISYIHLMAHFRMHTQIKNQTAALISGFRSIIKPEWIRMFSTPELQRLISGDNAEIDLEDLKKHTVYYGGFHGSHRVIIWLWDILASDFTPEERAMFLKFVTSCSRPPLLGFAYLKPPFSIRCVEVSDDQDTGDTLGSVLRGFFTIRKREPGGRLPTSSTCFNLLKLPNYSKKSVLREKLRYAISMNTGFELS

Target ORF information:

RefSeq Version	NM_054093.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus ubiquitin protein ligase E3B (Ube3b), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_054093.3

ORF Insert Sequence:

ATGTTCACTG TATCTCAGAC CTCCAGAGCA TGGTTCATCG ACAGAGCCCG GCAGGCACGA 
GAAGAGAGGC TGGTGCAGAA GGAGCGGGAG CGCTCAGCGG TGACGATCCA GGCCCTAGTC 
CGCAGTTTCC TCTGCCGGAG GCGACTGCAC AGAGACATCA GGAAAGAGAT TGATGAGTTT 
TTTAGTGCTG ATGAGTCTGG GTCCAGTAAA AGAAGTGCAC TTTGTATTTT TAAGATTGCC 
AGGAGACTGC TGTTTATATG CAAAACTACC GAGGACAGCG AGAGACTGGA GAAGTTGTGT 
CGAAGCATCC TGAACAGCAT GGATGCCGAG AATGAGCCAA AGGTGTGGTA CGTGTCCCTG 
GCTCTCTCCA AGGACCTCAC GCTGCTGTGG ATTAAGCAGA TCAAGAGCAT CCTGTGGCAC 
TGCTGTGAGC TTCTGGGGCA GCTCAAGCCA GAGATCTTAC AGGACTCCAG GCTCATCACG 
CTGTACCTCA CCATGCTTGT GACCTTCACA GACACTTCAA CATGGAAGAT TCTCCGGGGA 
AAAGGTGAGA GCCTTCGGCC AGCCCTGAAC CACATTTGCG CAAATATAAT GGGCCATCTC 
AACCAGCGTG GACTTTACTC TGTATTGCAG GTACTGCTGA CCCGAGGCCT GGCCAGGCCC 
CGGCCCTGTC TGTCCAAAGG CATGTTAACA GCAGCCTTCT CTCTGGCCCT GCGCCCTGTG 
GTTGCTGCGC AGTTCTCAGA CAATCTTATG CGACCATTCA TCATCCACGT CATGTCGGTA 
CCTGCTCTCG TGGCTCATCT CAGCACCGTG GCCCCTGAGC GCCTCGGCGT TTTAGAGTCC 
CATGACATGC TTCGTAAATT CATCGTCTTC CTGAGAGATA GAGACCGATG CCGCGATGCT 
TGTGAGAGTT TAGAAGGCTG CCATACCCTG TGTCTCATGG GCAATCTCCT GCACTTGGGC 
TCCCTCAGCC TCAGGCTGTT AGAGGAAGAG ATGGATGGGT TTGTGAGCGC GCTCACGCAG 
ATGCTGTGCT ACTGTCAGAA GTACGTGGCT CAGAAGAAGT CCAACCTGAC CCACTGGCAC 
CCTGTGCTCG GCTGGTTTTC CCAACCTGTG GATTACGGCC TCAATGATTC CATGTACCTG 
ATCACCAAGC AGCTGCAGTT CCTGTGGGCG GTGCCTCTCA TCCGCATTCT CTTCAGTGAC 
ATCCTGAGCA GGAAGCTGCT GGAGCATGCA GAGCCGGCCC CGGTGCAACC GCAGCCGTCC 
TCACCGCAGA CGGTGCTCCC CGTGAAGAGC CTCCTCAAGC GTGCGTTTCA GAAGTCGGCT 
TCGGTTCGGA ACATCCTCAG GCCTGTTGGG GGCCGCCGTG TCGACTCCGC TGAGGTGCGG 
AAGGTCTGCA ACATTTGCGT GCTCTACCAG ACCTCGCTGA CGACCCTCAC CCAGATCCGC 
CTGCAGATCC TCACAGGTCT TACGTACCTG GATGACCTGC TGCCCAAGCT CTGGGCGTTT 
ATCTGTGAGC TGGGGCCCCA CGGCGGGCTC AAGCTCTTCC TGGAGTGCCT GAACAATGAC 
ACTGGAGAGT CCAAGCAGCT CCTGGCCATG CTGATGCTCT TCTGTGACTG TTCCCGGCAC 
CTCATCACCA TCCTCGATGA CATTGAAGTT TATGAAGAAC AGATCTCCTT CAAGCTGGAA 
GAGCTGGTCA CCATCTCCTC GTTCCTCAAT TCCTTCGTGT TTAAGATGAT CTGGGACGGA 
ATTGTAGAGA ATGCCAAGGG TGAGACCTTG GAGCTGTTCC AGTCCGTCCA TGGCTGGCTG 
ATGGTGCTGT ATGAGCGAGA CTGCCGCCGG CGCTTTGCTC CGGAGGACCA CTGGCTACGG 
AGGGACCTCA AGCCTGGTGT GCTCTTCCAG GAGCTCGACA AGGACCGAAG GAGGGCTCAG 
CTGGTCCTGC AGCACATCCC CCACGTCGTG CCTCACAAGA ACAGAGTTCT TCTGTTCCGG 
AACATGGTTA TCAAGGAGAA AGAGAAGCTG GGGCTTGTGG AGACCAGCTC TGCCTCTCCT 
CACGTCACCC ACATCACCAT CCGAAGGTCC CGGATGTTGG AGGACGGCTA CGAACAGCTC 
CGGCAGCTTT CCCAGCATGC CATGAAGGGT GTGATCCGAG TGAAGTTTGT CAACGACCTC 
GGGGTGGACG AGGCTGGGAT TGACCAGGAT GGTGTCTTCA AGGAGTTTCT GGAAGAGATC 
ATCAAGAGGG TGTTTGACCC CGCACTCAAC CTGTTCAAGA CAACCAGCGG GGATGAGCGG 
CTGTACCCAT CACCCACGTC CTACATTCAC GAGAATTACC TGCAGCTCTT CGAGTTTGTG 
GGGAAGATGC TGGGAAAGGC AGTGTATGAG GGGATCGTGG TAGATGTGCC CTTTGCCTCC 
TTCTTCCTGA GCCAGATGCT CGGGCACCAC CACAGCGTCT TCTACAGCTC CGTGGATGAG 
CTTCCCTCCC TGGACTCGGA GTTTTATAAA AACCTCACCT CCATCAAGCG CTACGATGGA 
GACATCGCAG ACCTCGGGCT GACGCTGTCT TACGATGAAG ACGTCATGGG TCAGCTCGTC 
TGCCACGAAC TGGTTCCCGG AGGGAAGACC ATCCCGGTTA CAGATGAGAA CAAAATTAGC 
TACATCCATC TGATGGCGCA TTTCCGAATG CACACTCAAA TTAAAAACCA GACAGCTGCT 
CTTATCAGTG GCTTCCGTTC CATCATCAAG CCCGAGTGGA TCCGAATGTT CTCTACCCCT 
GAGCTTCAGC GCCTCATCTC CGGGGACAAC GCTGAGATAG ATCTGGAAGA CTTAAAGAAG 
CACACAGTGT ACTACGGTGG CTTTCATGGG AGCCACAGGG TCATCATCTG GCTCTGGGAT 
ATCCTGGCTT CCGACTTCAC GCCTGAAGAA AGAGCCATGT TCCTGAAGTT TGTGACCAGC 
TGCTCCCGGC CCCCGCTCCT AGGATTCGCC TACCTCAAGC CTCCGTTCTC CATCCGCTGT 
GTGGAAGTAT CAGATGACCA GGACACTGGG GATACCCTGG GCAGCGTCCT CAGGGGCTTC 
TTCACCATCC GCAAGAGGGA GCCAGGGGGG CGTCTGCCCA CATCTTCCAC TTGCTTCAAC 
CTGCTTAAGC TGCCCAACTA CAGCAAGAAG AGCGTACTGC GAGAGAAACT ACGCTATGCC 
ATCAGCATGA ACACGGGCTT TGAGCTCTCC TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

UBE3B ( XM_006530125.1 ) cDNA ORF clone, Mus musculus(House mouse) -> XP_006530188.1 Mus musculus ubiquitin protein ligase E3B (Ube3b), transcript variant X1, mRNA.

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CloneID	OMu09583
Clone ID Related Accession (Same CDS sequence)	NM_054093.2 , NM_054093.3 , XM_006530125.1
Accession Version	XM_006530125.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3213bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-02-08
Organism	Mus musculus(House mouse)
Product	ubiquitin-protein ligase E3B isoform X1
Comment

ATGTTCACTG TATCTCAGAC CTCCAGAGCA TGGTTCATCG ACAGAGCCCG GCAGGCACGA 
GAAGAGAGGC TGGTGCAGAA GGAGCGGGAG CGCTCAGCGG TGACGATCCA GGCCCTAGTC 
CGCAGTTTCC TCTGCCGGAG GCGACTGCAC AGAGACATCA GGAAAGAGAT TGATGAGTTT 
TTTAGTGCTG ATGAGTCTGG GTCCAGTAAA AGAAGTGCAC TTTGTATTTT TAAGATTGCC 
AGGAGACTGC TGTTTATATG CAAAACTACC GAGGACAGCG AGAGACTGGA GAAGTTGTGT 
CGAAGCATCC TGAACAGCAT GGATGCCGAG AATGAGCCAA AGGTGTGGTA CGTGTCCCTG 
GCTCTCTCCA AGGACCTCAC GCTGCTGTGG ATTAAGCAGA TCAAGAGCAT CCTGTGGCAC 
TGCTGTGAGC TTCTGGGGCA GCTCAAGCCA GAGATCTTAC AGGACTCCAG GCTCATCACG 
CTGTACCTCA CCATGCTTGT GACCTTCACA GACACTTCAA CATGGAAGAT TCTCCGGGGA 
AAAGGTGAGA GCCTTCGGCC AGCCCTGAAC CACATTTGCG CAAATATAAT GGGCCATCTC 
AACCAGCGTG GACTTTACTC TGTATTGCAG GTACTGCTGA CCCGAGGCCT GGCCAGGCCC 
CGGCCCTGTC TGTCCAAAGG CATGTTAACA GCAGCCTTCT CTCTGGCCCT GCGCCCTGTG 
GTTGCTGCGC AGTTCTCAGA CAATCTTATG CGACCATTCA TCATCCACGT CATGTCGGTA 
CCTGCTCTCG TGGCTCATCT CAGCACCGTG GCCCCTGAGC GCCTCGGCGT TTTAGAGTCC 
CATGACATGC TTCGTAAATT CATCGTCTTC CTGAGAGATA GAGACCGATG CCGCGATGCT 
TGTGAGAGTT TAGAAGGCTG CCATACCCTG TGTCTCATGG GCAATCTCCT GCACTTGGGC 
TCCCTCAGCC TCAGGCTGTT AGAGGAAGAG ATGGATGGGT TTGTGAGCGC GCTCACGCAG 
ATGCTGTGCT ACTGTCAGAA GTACGTGGCT CAGAAGAAGT CCAACCTGAC CCACTGGCAC 
CCTGTGCTCG GCTGGTTTTC CCAACCTGTG GATTACGGCC TCAATGATTC CATGTACCTG 
ATCACCAAGC AGCTGCAGTT CCTGTGGGCG GTGCCTCTCA TCCGCATTCT CTTCAGTGAC 
ATCCTGAGCA GGAAGCTGCT GGAGCATGCA GAGCCGGCCC CGGTGCAACC GCAGCCGTCC 
TCACCGCAGA CGGTGCTCCC CGTGAAGAGC CTCCTCAAGC GTGCGTTTCA GAAGTCGGCT 
TCGGTTCGGA ACATCCTCAG GCCTGTTGGG GGCCGCCGTG TCGACTCCGC TGAGGTGCGG 
AAGGTCTGCA ACATTTGCGT GCTCTACCAG ACCTCGCTGA CGACCCTCAC CCAGATCCGC 
CTGCAGATCC TCACAGGTCT TACGTACCTG GATGACCTGC TGCCCAAGCT CTGGGCGTTT 
ATCTGTGAGC TGGGGCCCCA CGGCGGGCTC AAGCTCTTCC TGGAGTGCCT GAACAATGAC 
ACTGGAGAGT CCAAGCAGCT CCTGGCCATG CTGATGCTCT TCTGTGACTG TTCCCGGCAC 
CTCATCACCA TCCTCGATGA CATTGAAGTT TATGAAGAAC AGATCTCCTT CAAGCTGGAA 
GAGCTGGTCA CCATCTCCTC GTTCCTCAAT TCCTTCGTGT TTAAGATGAT CTGGGACGGA 
ATTGTAGAGA ATGCCAAGGG TGAGACCTTG GAGCTGTTCC AGTCCGTCCA TGGCTGGCTG 
ATGGTGCTGT ATGAGCGAGA CTGCCGCCGG CGCTTTGCTC CGGAGGACCA CTGGCTACGG 
AGGGACCTCA AGCCTGGTGT GCTCTTCCAG GAGCTCGACA AGGACCGAAG GAGGGCTCAG 
CTGGTCCTGC AGCACATCCC CCACGTCGTG CCTCACAAGA ACAGAGTTCT TCTGTTCCGG 
AACATGGTTA TCAAGGAGAA AGAGAAGCTG GGGCTTGTGG AGACCAGCTC TGCCTCTCCT 
CACGTCACCC ACATCACCAT CCGAAGGTCC CGGATGTTGG AGGACGGCTA CGAACAGCTC 
CGGCAGCTTT CCCAGCATGC CATGAAGGGT GTGATCCGAG TGAAGTTTGT CAACGACCTC 
GGGGTGGACG AGGCTGGGAT TGACCAGGAT GGTGTCTTCA AGGAGTTTCT GGAAGAGATC 
ATCAAGAGGG TGTTTGACCC CGCACTCAAC CTGTTCAAGA CAACCAGCGG GGATGAGCGG 
CTGTACCCAT CACCCACGTC CTACATTCAC GAGAATTACC TGCAGCTCTT CGAGTTTGTG 
GGGAAGATGC TGGGAAAGGC AGTGTATGAG GGGATCGTGG TAGATGTGCC CTTTGCCTCC 
TTCTTCCTGA GCCAGATGCT CGGGCACCAC CACAGCGTCT TCTACAGCTC CGTGGATGAG 
CTTCCCTCCC TGGACTCGGA GTTTTATAAA AACCTCACCT CCATCAAGCG CTACGATGGA 
GACATCGCAG ACCTCGGGCT GACGCTGTCT TACGATGAAG ACGTCATGGG TCAGCTCGTC 
TGCCACGAAC TGGTTCCCGG AGGGAAGACC ATCCCGGTTA CAGATGAGAA CAAAATTAGC 
TACATCCATC TGATGGCGCA TTTCCGAATG CACACTCAAA TTAAAAACCA GACAGCTGCT 
CTTATCAGTG GCTTCCGTTC CATCATCAAG CCCGAGTGGA TCCGAATGTT CTCTACCCCT 
GAGCTTCAGC GCCTCATCTC CGGGGACAAC GCTGAGATAG ATCTGGAAGA CTTAAAGAAG 
CACACAGTGT ACTACGGTGG CTTTCATGGG AGCCACAGGG TCATCATCTG GCTCTGGGAT 
ATCCTGGCTT CCGACTTCAC GCCTGAAGAA AGAGCCATGT TCCTGAAGTT TGTGACCAGC 
TGCTCCCGGC CCCCGCTCCT AGGATTCGCC TACCTCAAGC CTCCGTTCTC CATCCGCTGT 
GTGGAAGTAT CAGATGACCA GGACACTGGG GATACCCTGG GCAGCGTCCT CAGGGGCTTC 
TTCACCATCC GCAAGAGGGA GCCAGGGGGG CGTCTGCCCA CATCTTCCAC TTGCTTCAAC 
CTGCTTAAGC TGCCCAACTA CAGCAAGAAG AGCGTACTGC GAGAGAAACT ACGCTATGCC 
ATCAGCATGA ACACGGGCTT TGAGCTCTCC TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq

XP_006530188.1

Translation

MFTVSQTSRAWFIDRARQAREERLVQKERERSAVTIQALVRSFLCRRRLHRDIRKEIDEFFSADESGSSKRSALCIFKIARRLLFICKTTEDSERLEKLCRSILNSMDAENEPKVWYVSLALSKDLTLLWIKQIKSILWHCCELLGQLKPEILQDSRLITLYLTMLVTFTDTSTWKILRGKGESLRPALNHICANIMGHLNQRGLYSVLQVLLTRGLARPRPCLSKGMLTAAFSLALRPVVAAQFSDNLMRPFIIHVMSVPALVAHLSTVAPERLGVLESHDMLRKFIVFLRDRDRCRDACESLEGCHTLCLMGNLLHLGSLSLRLLEEEMDGFVSALTQMLCYCQKYVAQKKSNLTHWHPVLGWFSQPVDYGLNDSMYLITKQLQFLWAVPLIRILFSDILSRKLLEHAEPAPVQPQPSSPQTVLPVKSLLKRAFQKSASVRNILRPVGGRRVDSAEVRKVCNICVLYQTSLTTLTQIRLQILTGLTYLDDLLPKLWAFICELGPHGGLKLFLECLNNDTGESKQLLAMLMLFCDCSRHLITILDDIEVYEEQISFKLEELVTISSFLNSFVFKMIWDGIVENAKGETLELFQSVHGWLMVLYERDCRRRFAPEDHWLRRDLKPGVLFQELDKDRRRAQLVLQHIPHVVPHKNRVLLFRNMVIKEKEKLGLVETSSASPHVTHITIRRSRMLEDGYEQLRQLSQHAMKGVIRVKFVNDLGVDEAGIDQDGVFKEFLEEIIKRVFDPALNLFKTTSGDERLYPSPTSYIHENYLQLFEFVGKMLGKAVYEGIVVDVPFASFFLSQMLGHHHSVFYSSVDELPSLDSEFYKNLTSIKRYDGDIADLGLTLSYDEDVMGQLVCHELVPGGKTIPVTDENKISYIHLMAHFRMHTQIKNQTAALISGFRSIIKPEWIRMFSTPELQRLISGDNAEIDLEDLKKHTVYYGGFHGSHRVIIWLWDILASDFTPEERAMFLKFVTSCSRPPLLGFAYLKPPFSIRCVEVSDDQDTGDTLGSVLRGFFTIRKREPGGRLPTSSTCFNLLKLPNYSKKSVLREKLRYAISMNTGFELS

Target ORF information:

RefSeq Version	XM_006530125.1
Organism	Mus musculus(House mouse)
Definition	Mus musculus ubiquitin protein ligase E3B (Ube3b), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006530125.1

ORF Insert Sequence:

ATGTTCACTG TATCTCAGAC CTCCAGAGCA TGGTTCATCG ACAGAGCCCG GCAGGCACGA 
GAAGAGAGGC TGGTGCAGAA GGAGCGGGAG CGCTCAGCGG TGACGATCCA GGCCCTAGTC 
CGCAGTTTCC TCTGCCGGAG GCGACTGCAC AGAGACATCA GGAAAGAGAT TGATGAGTTT 
TTTAGTGCTG ATGAGTCTGG GTCCAGTAAA AGAAGTGCAC TTTGTATTTT TAAGATTGCC 
AGGAGACTGC TGTTTATATG CAAAACTACC GAGGACAGCG AGAGACTGGA GAAGTTGTGT 
CGAAGCATCC TGAACAGCAT GGATGCCGAG AATGAGCCAA AGGTGTGGTA CGTGTCCCTG 
GCTCTCTCCA AGGACCTCAC GCTGCTGTGG ATTAAGCAGA TCAAGAGCAT CCTGTGGCAC 
TGCTGTGAGC TTCTGGGGCA GCTCAAGCCA GAGATCTTAC AGGACTCCAG GCTCATCACG 
CTGTACCTCA CCATGCTTGT GACCTTCACA GACACTTCAA CATGGAAGAT TCTCCGGGGA 
AAAGGTGAGA GCCTTCGGCC AGCCCTGAAC CACATTTGCG CAAATATAAT GGGCCATCTC 
AACCAGCGTG GACTTTACTC TGTATTGCAG GTACTGCTGA CCCGAGGCCT GGCCAGGCCC 
CGGCCCTGTC TGTCCAAAGG CATGTTAACA GCAGCCTTCT CTCTGGCCCT GCGCCCTGTG 
GTTGCTGCGC AGTTCTCAGA CAATCTTATG CGACCATTCA TCATCCACGT CATGTCGGTA 
CCTGCTCTCG TGGCTCATCT CAGCACCGTG GCCCCTGAGC GCCTCGGCGT TTTAGAGTCC 
CATGACATGC TTCGTAAATT CATCGTCTTC CTGAGAGATA GAGACCGATG CCGCGATGCT 
TGTGAGAGTT TAGAAGGCTG CCATACCCTG TGTCTCATGG GCAATCTCCT GCACTTGGGC 
TCCCTCAGCC TCAGGCTGTT AGAGGAAGAG ATGGATGGGT TTGTGAGCGC GCTCACGCAG 
ATGCTGTGCT ACTGTCAGAA GTACGTGGCT CAGAAGAAGT CCAACCTGAC CCACTGGCAC 
CCTGTGCTCG GCTGGTTTTC CCAACCTGTG GATTACGGCC TCAATGATTC CATGTACCTG 
ATCACCAAGC AGCTGCAGTT CCTGTGGGCG GTGCCTCTCA TCCGCATTCT CTTCAGTGAC 
ATCCTGAGCA GGAAGCTGCT GGAGCATGCA GAGCCGGCCC CGGTGCAACC GCAGCCGTCC 
TCACCGCAGA CGGTGCTCCC CGTGAAGAGC CTCCTCAAGC GTGCGTTTCA GAAGTCGGCT 
TCGGTTCGGA ACATCCTCAG GCCTGTTGGG GGCCGCCGTG TCGACTCCGC TGAGGTGCGG 
AAGGTCTGCA ACATTTGCGT GCTCTACCAG ACCTCGCTGA CGACCCTCAC CCAGATCCGC 
CTGCAGATCC TCACAGGTCT TACGTACCTG GATGACCTGC TGCCCAAGCT CTGGGCGTTT 
ATCTGTGAGC TGGGGCCCCA CGGCGGGCTC AAGCTCTTCC TGGAGTGCCT GAACAATGAC 
ACTGGAGAGT CCAAGCAGCT CCTGGCCATG CTGATGCTCT TCTGTGACTG TTCCCGGCAC 
CTCATCACCA TCCTCGATGA CATTGAAGTT TATGAAGAAC AGATCTCCTT CAAGCTGGAA 
GAGCTGGTCA CCATCTCCTC GTTCCTCAAT TCCTTCGTGT TTAAGATGAT CTGGGACGGA 
ATTGTAGAGA ATGCCAAGGG TGAGACCTTG GAGCTGTTCC AGTCCGTCCA TGGCTGGCTG 
ATGGTGCTGT ATGAGCGAGA CTGCCGCCGG CGCTTTGCTC CGGAGGACCA CTGGCTACGG 
AGGGACCTCA AGCCTGGTGT GCTCTTCCAG GAGCTCGACA AGGACCGAAG GAGGGCTCAG 
CTGGTCCTGC AGCACATCCC CCACGTCGTG CCTCACAAGA ACAGAGTTCT TCTGTTCCGG 
AACATGGTTA TCAAGGAGAA AGAGAAGCTG GGGCTTGTGG AGACCAGCTC TGCCTCTCCT 
CACGTCACCC ACATCACCAT CCGAAGGTCC CGGATGTTGG AGGACGGCTA CGAACAGCTC 
CGGCAGCTTT CCCAGCATGC CATGAAGGGT GTGATCCGAG TGAAGTTTGT CAACGACCTC 
GGGGTGGACG AGGCTGGGAT TGACCAGGAT GGTGTCTTCA AGGAGTTTCT GGAAGAGATC 
ATCAAGAGGG TGTTTGACCC CGCACTCAAC CTGTTCAAGA CAACCAGCGG GGATGAGCGG 
CTGTACCCAT CACCCACGTC CTACATTCAC GAGAATTACC TGCAGCTCTT CGAGTTTGTG 
GGGAAGATGC TGGGAAAGGC AGTGTATGAG GGGATCGTGG TAGATGTGCC CTTTGCCTCC 
TTCTTCCTGA GCCAGATGCT CGGGCACCAC CACAGCGTCT TCTACAGCTC CGTGGATGAG 
CTTCCCTCCC TGGACTCGGA GTTTTATAAA AACCTCACCT CCATCAAGCG CTACGATGGA 
GACATCGCAG ACCTCGGGCT GACGCTGTCT TACGATGAAG ACGTCATGGG TCAGCTCGTC 
TGCCACGAAC TGGTTCCCGG AGGGAAGACC ATCCCGGTTA CAGATGAGAA CAAAATTAGC 
TACATCCATC TGATGGCGCA TTTCCGAATG CACACTCAAA TTAAAAACCA GACAGCTGCT 
CTTATCAGTG GCTTCCGTTC CATCATCAAG CCCGAGTGGA TCCGAATGTT CTCTACCCCT 
GAGCTTCAGC GCCTCATCTC CGGGGACAAC GCTGAGATAG ATCTGGAAGA CTTAAAGAAG 
CACACAGTGT ACTACGGTGG CTTTCATGGG AGCCACAGGG TCATCATCTG GCTCTGGGAT 
ATCCTGGCTT CCGACTTCAC GCCTGAAGAA AGAGCCATGT TCCTGAAGTT TGTGACCAGC 
TGCTCCCGGC CCCCGCTCCT AGGATTCGCC TACCTCAAGC CTCCGTTCTC CATCCGCTGT 
GTGGAAGTAT CAGATGACCA GGACACTGGG GATACCCTGG GCAGCGTCCT CAGGGGCTTC 
TTCACCATCC GCAAGAGGGA GCCAGGGGGG CGTCTGCCCA CATCTTCCAC TTGCTTCAAC 
CTGCTTAAGC TGCCCAACTA CAGCAAGAAG AGCGTACTGC GAGAGAAACT ACGCTATGCC 
ATCAGCATGA ACACGGGCTT TGAGCTCTCC TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

UBE3B ( XM_006530126.3 ) cDNA ORF clone, Mus musculus(House mouse) -> XP_006530189.1 Mus musculus ubiquitin protein ligase E3B (Ube3b), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu35771
Clone ID Related Accession (Same CDS sequence)	XM_006530126.2 , XM_006530126.3
Accession Version	XM_006530126.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3063bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	ubiquitin-protein ligase E3B isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000071.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006530126.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTTCATC GACAGAGCCC GGCAGGCACG AGAAGAGAGG CTGGTGCAGA AGGAGCGGGA 
GCGCTCAGCG GTGACGATCC AGGCCCTAGT CCGCAGTTTC CTCTGCCGGA GGCGACTGCA 
CAGAGACATC AGAGACTGGA GAAGTTGTGT CGAAGCATCC TGAACAGCAT GGATGCCGAG 
AATGAGCCAA AGGTGTGGTA CGTGTCCCTG GCTCTCTCCA AGGACCTCAC GCTGCTGTGG 
ATTAAGCAGA TCAAGAGCAT CCTGTGGCAC TGCTGTGAGC TTCTGGGGCA GCTCAAGCCA 
GAGATCTTAC AGGACTCCAG GCTCATCACG CTGTACCTCA CCATGCTTGT GACCTTCACA 
GACACTTCAA CATGGAAGAT TCTCCGGGGA AAAGGTGAGA GCCTTCGGCC AGCCCTGAAC 
CACATTTGCG CAAATATAAT GGGCCATCTC AACCAGCGTG GACTTTACTC TGTATTGCAG 
GTACTGCTGA CCCGAGGCCT GGCCAGGCCC CGGCCCTGTC TGTCCAAAGG CATGTTAACA 
GCAGCCTTCT CTCTGGCCCT GCGCCCTGTG GTTGCTGCGC AGTTCTCAGA CAATCTTATG 
CGACCATTCA TCATCCACGT CATGTCGGTA CCTGCTCTCG TGGCTCATCT CAGCACCGTG 
GCCCCTGAGC GCCTCGGCGT TTTAGAGTCC CATGACATGC TTCGTAAATT CATCGTCTTC 
CTGAGAGATA GAGACCGATG CCGCGATGCT TGTGAGAGTT TAGAAGGCTG CCATACCCTG 
TGTCTCATGG GCAATCTCCT GCACTTGGGC TCCCTCAGCC TCAGGCTGTT AGAGGAAGAG 
ATGGATGGGT TTGTGAGCGC GCTCACGCAG ATGCTGTGCT ACTGTCAGAA GTACGTGGCT 
CAGAAGAAGT CCAACCTGAC CCACTGGCAC CCTGTGCTCG GCTGGTTTTC CCAACCTGTG 
GATTACGGCC TCAATGATTC CATGTACCTG ATCACCAAGC AGCTGCAGTT CCTGTGGGCG 
GTGCCTCTCA TCCGCATTCT CTTCAGTGAC ATCCTGAGCA GGAAGCTGCT GGAGCATGCA 
GAGCCGGCCC CGGTGCAACC GCAGCCGTCC TCACCGCAGA CGGTGCTCCC CGTGAAGAGC 
CTCCTCAAGC GTGCGTTTCA GAAGTCGGCT TCGGTTCGGA ACATCCTCAG GCCTGTTGGG 
GGCCGCCGTG TCGACTCCGC TGAGGTGCGG AAGGTCTGCA ACATTTGCGT GCTCTACCAG 
ACCTCGCTGA CGACCCTCAC CCAGATCCGC CTGCAGATCC TCACAGGTCT TACGTACCTG 
GATGACCTGC TGCCCAAGCT CTGGGCGTTT ATCTGTGAGC TGGGGCCCCA CGGCGGGCTC 
AAGCTCTTCC TGGAGTGCCT GAACAATGAC ACTGGAGAGT CCAAGCAGCT CCTGGCCATG 
CTGATGCTCT TCTGTGACTG TTCCCGGCAC CTCATCACCA TCCTCGATGA CATTGAAGTT 
TATGAAGAAC AGATCTCCTT CAAGCTGGAA GAGCTGGTCA CCATCTCCTC GTTCCTCAAT 
TCCTTCGTGT TTAAGATGAT CTGGGACGGA ATTGTAGAGA ATGCCAAGGG TGAGACCTTG 
GAGCTGTTCC AGTCCGTCCA TGGCTGGCTG ATGGTGCTGT ATGAGCGAGA CTGCCGCCGG 
CGCTTTGCTC CGGAGGACCA CTGGCTACGG AGGGACCTCA AGCCTGGTGT GCTCTTCCAG 
GAGCTCGACA AGGACCGAAG GAGGGCTCAG CTGGTCCTGC AGCACATCCC CCACGTCGTG 
CCTCACAAGA ACAGAGTTCT TCTGTTCCGG AACATGGTTA TCAAGGAGAA AGAGAAGCTG 
GGGCTTGTGG AGACCAGCTC TGCCTCTCCT CACGTCACCC ACATCACCAT CCGAAGGTCC 
CGGATGTTGG AGGACGGCTA CGAACAGCTC CGGCAGCTTT CCCAGCATGC CATGAAGGGT 
GTGATCCGAG TGAAGTTTGT CAACGACCTC GGGGTGGACG AGGCTGGGAT TGACCAGGAT 
GGTGTCTTCA AGGAGTTTCT GGAAGAGATC ATCAAGAGGG TGTTTGACCC CGCACTCAAC 
CTGTTCAAGA CAACCAGCGG GGATGAGCGG CTGTACCCAT CACCCACGTC CTACATTCAC 
GAGAATTACC TGCAGCTCTT CGAGTTTGTG GGGAAGATGC TGGGAAAGGC AGTGTATGAG 
GGGATCGTGG TAGATGTGCC CTTTGCCTCC TTCTTCCTGA GCCAGATGCT CGGGCACCAC 
CACAGCGTCT TCTACAGCTC CGTGGATGAG CTTCCCTCCC TGGACTCGGA GTTTTATAAA 
AACCTCACCT CCATCAAGCG CTACGATGGA GACATCGCAG ACCTCGGGCT GACGCTGTCT 
TACGATGAAG ACGTCATGGG TCAGCTCGTC TGCCACGAAC TGGTTCCCGG AGGGAAGACC 
ATCCCGGTTA CAGATGAGAA CAAAATTAGC TACATCCATC TGATGGCGCA TTTCCGAATG 
CACACTCAAA TTAAAAACCA GACAGCTGCT CTTATCAGTG GCTTCCGTTC CATCATCAAG 
CCCGAGTGGA TCCGAATGTT CTCTACCCCT GAGCTTCAGC GCCTCATCTC CGGGGACAAC 
GCTGAGATAG ATCTGGAAGA CTTAAAGAAG CACACAGTGT ACTACGGTGG CTTTCATGGG 
AGCCACAGGG TCATCATCTG GCTCTGGGAT ATCCTGGCTT CCGACTTCAC GCCTGAAGAA 
AGAGCCATGT TCCTGAAGTT TGTGACCAGC TGCTCCCGGC CCCCGCTCCT AGGATTCGCC 
TACCTCAAGC CTCCGTTCTC CATCCGCTGT GTGGAAGTAT CAGATGACCA GGACACTGGG 
GATACCCTGG GCAGCGTCCT CAGGGGCTTC TTCACCATCC GCAAGAGGGA GCCAGGGGGG 
CGTCTGCCCA CATCTTCCAC TTGCTTCAAC CTGCTTAAGC TGCCCAACTA CAGCAAGAAG 
AGCGTACTGC GAGAGAAACT ACGCTATGCC ATCAGCATGA ACACGGGCTT TGAGCTCTCC 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006530189.1
CDS	512..3574
Translation	MVHRQSPAGTRREAGAEGAGALSGDDPGPSPQFPLPEATAQRHQRLEKLCRSILNSMDAENEPKVWYVSLALSKDLTLLWIKQIKSILWHCCELLGQLKPEILQDSRLITLYLTMLVTFTDTSTWKILRGKGESLRPALNHICANIMGHLNQRGLYSVLQVLLTRGLARPRPCLSKGMLTAAFSLALRPVVAAQFSDNLMRPFIIHVMSVPALVAHLSTVAPERLGVLESHDMLRKFIVFLRDRDRCRDACESLEGCHTLCLMGNLLHLGSLSLRLLEEEMDGFVSALTQMLCYCQKYVAQKKSNLTHWHPVLGWFSQPVDYGLNDSMYLITKQLQFLWAVPLIRILFSDILSRKLLEHAEPAPVQPQPSSPQTVLPVKSLLKRAFQKSASVRNILRPVGGRRVDSAEVRKVCNICVLYQTSLTTLTQIRLQILTGLTYLDDLLPKLWAFICELGPHGGLKLFLECLNNDTGESKQLLAMLMLFCDCSRHLITILDDIEVYEEQISFKLEELVTISSFLNSFVFKMIWDGIVENAKGETLELFQSVHGWLMVLYERDCRRRFAPEDHWLRRDLKPGVLFQELDKDRRRAQLVLQHIPHVVPHKNRVLLFRNMVIKEKEKLGLVETSSASPHVTHITIRRSRMLEDGYEQLRQLSQHAMKGVIRVKFVNDLGVDEAGIDQDGVFKEFLEEIIKRVFDPALNLFKTTSGDERLYPSPTSYIHENYLQLFEFVGKMLGKAVYEGIVVDVPFASFFLSQMLGHHHSVFYSSVDELPSLDSEFYKNLTSIKRYDGDIADLGLTLSYDEDVMGQLVCHELVPGGKTIPVTDENKISYIHLMAHFRMHTQIKNQTAALISGFRSIIKPEWIRMFSTPELQRLISGDNAEIDLEDLKKHTVYYGGFHGSHRVIIWLWDILASDFTPEERAMFLKFVTSCSRPPLLGFAYLKPPFSIRCVEVSDDQDTGDTLGSVLRGFFTIRKREPGGRLPTSSTCFNLLKLPNYSKKSVLREKLRYAISMNTGFELS

Target ORF information:

RefSeq Version	XM_006530126.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus ubiquitin protein ligase E3B (Ube3b), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006530126.3

ORF Insert Sequence:

ATGGTTCATC GACAGAGCCC GGCAGGCACG AGAAGAGAGG CTGGTGCAGA AGGAGCGGGA 
GCGCTCAGCG GTGACGATCC AGGCCCTAGT CCGCAGTTTC CTCTGCCGGA GGCGACTGCA 
CAGAGACATC AGAGACTGGA GAAGTTGTGT CGAAGCATCC TGAACAGCAT GGATGCCGAG 
AATGAGCCAA AGGTGTGGTA CGTGTCCCTG GCTCTCTCCA AGGACCTCAC GCTGCTGTGG 
ATTAAGCAGA TCAAGAGCAT CCTGTGGCAC TGCTGTGAGC TTCTGGGGCA GCTCAAGCCA 
GAGATCTTAC AGGACTCCAG GCTCATCACG CTGTACCTCA CCATGCTTGT GACCTTCACA 
GACACTTCAA CATGGAAGAT TCTCCGGGGA AAAGGTGAGA GCCTTCGGCC AGCCCTGAAC 
CACATTTGCG CAAATATAAT GGGCCATCTC AACCAGCGTG GACTTTACTC TGTATTGCAG 
GTACTGCTGA CCCGAGGCCT GGCCAGGCCC CGGCCCTGTC TGTCCAAAGG CATGTTAACA 
GCAGCCTTCT CTCTGGCCCT GCGCCCTGTG GTTGCTGCGC AGTTCTCAGA CAATCTTATG 
CGACCATTCA TCATCCACGT CATGTCGGTA CCTGCTCTCG TGGCTCATCT CAGCACCGTG 
GCCCCTGAGC GCCTCGGCGT TTTAGAGTCC CATGACATGC TTCGTAAATT CATCGTCTTC 
CTGAGAGATA GAGACCGATG CCGCGATGCT TGTGAGAGTT TAGAAGGCTG CCATACCCTG 
TGTCTCATGG GCAATCTCCT GCACTTGGGC TCCCTCAGCC TCAGGCTGTT AGAGGAAGAG 
ATGGATGGGT TTGTGAGCGC GCTCACGCAG ATGCTGTGCT ACTGTCAGAA GTACGTGGCT 
CAGAAGAAGT CCAACCTGAC CCACTGGCAC CCTGTGCTCG GCTGGTTTTC CCAACCTGTG 
GATTACGGCC TCAATGATTC CATGTACCTG ATCACCAAGC AGCTGCAGTT CCTGTGGGCG 
GTGCCTCTCA TCCGCATTCT CTTCAGTGAC ATCCTGAGCA GGAAGCTGCT GGAGCATGCA 
GAGCCGGCCC CGGTGCAACC GCAGCCGTCC TCACCGCAGA CGGTGCTCCC CGTGAAGAGC 
CTCCTCAAGC GTGCGTTTCA GAAGTCGGCT TCGGTTCGGA ACATCCTCAG GCCTGTTGGG 
GGCCGCCGTG TCGACTCCGC TGAGGTGCGG AAGGTCTGCA ACATTTGCGT GCTCTACCAG 
ACCTCGCTGA CGACCCTCAC CCAGATCCGC CTGCAGATCC TCACAGGTCT TACGTACCTG 
GATGACCTGC TGCCCAAGCT CTGGGCGTTT ATCTGTGAGC TGGGGCCCCA CGGCGGGCTC 
AAGCTCTTCC TGGAGTGCCT GAACAATGAC ACTGGAGAGT CCAAGCAGCT CCTGGCCATG 
CTGATGCTCT TCTGTGACTG TTCCCGGCAC CTCATCACCA TCCTCGATGA CATTGAAGTT 
TATGAAGAAC AGATCTCCTT CAAGCTGGAA GAGCTGGTCA CCATCTCCTC GTTCCTCAAT 
TCCTTCGTGT TTAAGATGAT CTGGGACGGA ATTGTAGAGA ATGCCAAGGG TGAGACCTTG 
GAGCTGTTCC AGTCCGTCCA TGGCTGGCTG ATGGTGCTGT ATGAGCGAGA CTGCCGCCGG 
CGCTTTGCTC CGGAGGACCA CTGGCTACGG AGGGACCTCA AGCCTGGTGT GCTCTTCCAG 
GAGCTCGACA AGGACCGAAG GAGGGCTCAG CTGGTCCTGC AGCACATCCC CCACGTCGTG 
CCTCACAAGA ACAGAGTTCT TCTGTTCCGG AACATGGTTA TCAAGGAGAA AGAGAAGCTG 
GGGCTTGTGG AGACCAGCTC TGCCTCTCCT CACGTCACCC ACATCACCAT CCGAAGGTCC 
CGGATGTTGG AGGACGGCTA CGAACAGCTC CGGCAGCTTT CCCAGCATGC CATGAAGGGT 
GTGATCCGAG TGAAGTTTGT CAACGACCTC GGGGTGGACG AGGCTGGGAT TGACCAGGAT 
GGTGTCTTCA AGGAGTTTCT GGAAGAGATC ATCAAGAGGG TGTTTGACCC CGCACTCAAC 
CTGTTCAAGA CAACCAGCGG GGATGAGCGG CTGTACCCAT CACCCACGTC CTACATTCAC 
GAGAATTACC TGCAGCTCTT CGAGTTTGTG GGGAAGATGC TGGGAAAGGC AGTGTATGAG 
GGGATCGTGG TAGATGTGCC CTTTGCCTCC TTCTTCCTGA GCCAGATGCT CGGGCACCAC 
CACAGCGTCT TCTACAGCTC CGTGGATGAG CTTCCCTCCC TGGACTCGGA GTTTTATAAA 
AACCTCACCT CCATCAAGCG CTACGATGGA GACATCGCAG ACCTCGGGCT GACGCTGTCT 
TACGATGAAG ACGTCATGGG TCAGCTCGTC TGCCACGAAC TGGTTCCCGG AGGGAAGACC 
ATCCCGGTTA CAGATGAGAA CAAAATTAGC TACATCCATC TGATGGCGCA TTTCCGAATG 
CACACTCAAA TTAAAAACCA GACAGCTGCT CTTATCAGTG GCTTCCGTTC CATCATCAAG 
CCCGAGTGGA TCCGAATGTT CTCTACCCCT GAGCTTCAGC GCCTCATCTC CGGGGACAAC 
GCTGAGATAG ATCTGGAAGA CTTAAAGAAG CACACAGTGT ACTACGGTGG CTTTCATGGG 
AGCCACAGGG TCATCATCTG GCTCTGGGAT ATCCTGGCTT CCGACTTCAC GCCTGAAGAA 
AGAGCCATGT TCCTGAAGTT TGTGACCAGC TGCTCCCGGC CCCCGCTCCT AGGATTCGCC 
TACCTCAAGC CTCCGTTCTC CATCCGCTGT GTGGAAGTAT CAGATGACCA GGACACTGGG 
GATACCCTGG GCAGCGTCCT CAGGGGCTTC TTCACCATCC GCAAGAGGGA GCCAGGGGGG 
CGTCTGCCCA CATCTTCCAC TTGCTTCAAC CTGCTTAAGC TGCCCAACTA CAGCAAGAAG 
AGCGTACTGC GAGAGAAACT ACGCTATGCC ATCAGCATGA ACACGGGCTT TGAGCTCTCC 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

UBE3B ( XM_006530126.2 ) cDNA ORF clone, Mus musculus(House mouse) -> XP_006530189.1 Mus musculus ubiquitin protein ligase E3B (Ube3b), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu35771
Clone ID Related Accession (Same CDS sequence)	XM_006530126.2 , XM_006530126.3
Accession Version	XM_006530126.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3063bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	ubiquitin-protein ligase E3B isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000071.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006530126.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTTCATC GACAGAGCCC GGCAGGCACG AGAAGAGAGG CTGGTGCAGA AGGAGCGGGA 
GCGCTCAGCG GTGACGATCC AGGCCCTAGT CCGCAGTTTC CTCTGCCGGA GGCGACTGCA 
CAGAGACATC AGAGACTGGA GAAGTTGTGT CGAAGCATCC TGAACAGCAT GGATGCCGAG 
AATGAGCCAA AGGTGTGGTA CGTGTCCCTG GCTCTCTCCA AGGACCTCAC GCTGCTGTGG 
ATTAAGCAGA TCAAGAGCAT CCTGTGGCAC TGCTGTGAGC TTCTGGGGCA GCTCAAGCCA 
GAGATCTTAC AGGACTCCAG GCTCATCACG CTGTACCTCA CCATGCTTGT GACCTTCACA 
GACACTTCAA CATGGAAGAT TCTCCGGGGA AAAGGTGAGA GCCTTCGGCC AGCCCTGAAC 
CACATTTGCG CAAATATAAT GGGCCATCTC AACCAGCGTG GACTTTACTC TGTATTGCAG 
GTACTGCTGA CCCGAGGCCT GGCCAGGCCC CGGCCCTGTC TGTCCAAAGG CATGTTAACA 
GCAGCCTTCT CTCTGGCCCT GCGCCCTGTG GTTGCTGCGC AGTTCTCAGA CAATCTTATG 
CGACCATTCA TCATCCACGT CATGTCGGTA CCTGCTCTCG TGGCTCATCT CAGCACCGTG 
GCCCCTGAGC GCCTCGGCGT TTTAGAGTCC CATGACATGC TTCGTAAATT CATCGTCTTC 
CTGAGAGATA GAGACCGATG CCGCGATGCT TGTGAGAGTT TAGAAGGCTG CCATACCCTG 
TGTCTCATGG GCAATCTCCT GCACTTGGGC TCCCTCAGCC TCAGGCTGTT AGAGGAAGAG 
ATGGATGGGT TTGTGAGCGC GCTCACGCAG ATGCTGTGCT ACTGTCAGAA GTACGTGGCT 
CAGAAGAAGT CCAACCTGAC CCACTGGCAC CCTGTGCTCG GCTGGTTTTC CCAACCTGTG 
GATTACGGCC TCAATGATTC CATGTACCTG ATCACCAAGC AGCTGCAGTT CCTGTGGGCG 
GTGCCTCTCA TCCGCATTCT CTTCAGTGAC ATCCTGAGCA GGAAGCTGCT GGAGCATGCA 
GAGCCGGCCC CGGTGCAACC GCAGCCGTCC TCACCGCAGA CGGTGCTCCC CGTGAAGAGC 
CTCCTCAAGC GTGCGTTTCA GAAGTCGGCT TCGGTTCGGA ACATCCTCAG GCCTGTTGGG 
GGCCGCCGTG TCGACTCCGC TGAGGTGCGG AAGGTCTGCA ACATTTGCGT GCTCTACCAG 
ACCTCGCTGA CGACCCTCAC CCAGATCCGC CTGCAGATCC TCACAGGTCT TACGTACCTG 
GATGACCTGC TGCCCAAGCT CTGGGCGTTT ATCTGTGAGC TGGGGCCCCA CGGCGGGCTC 
AAGCTCTTCC TGGAGTGCCT GAACAATGAC ACTGGAGAGT CCAAGCAGCT CCTGGCCATG 
CTGATGCTCT TCTGTGACTG TTCCCGGCAC CTCATCACCA TCCTCGATGA CATTGAAGTT 
TATGAAGAAC AGATCTCCTT CAAGCTGGAA GAGCTGGTCA CCATCTCCTC GTTCCTCAAT 
TCCTTCGTGT TTAAGATGAT CTGGGACGGA ATTGTAGAGA ATGCCAAGGG TGAGACCTTG 
GAGCTGTTCC AGTCCGTCCA TGGCTGGCTG ATGGTGCTGT ATGAGCGAGA CTGCCGCCGG 
CGCTTTGCTC CGGAGGACCA CTGGCTACGG AGGGACCTCA AGCCTGGTGT GCTCTTCCAG 
GAGCTCGACA AGGACCGAAG GAGGGCTCAG CTGGTCCTGC AGCACATCCC CCACGTCGTG 
CCTCACAAGA ACAGAGTTCT TCTGTTCCGG AACATGGTTA TCAAGGAGAA AGAGAAGCTG 
GGGCTTGTGG AGACCAGCTC TGCCTCTCCT CACGTCACCC ACATCACCAT CCGAAGGTCC 
CGGATGTTGG AGGACGGCTA CGAACAGCTC CGGCAGCTTT CCCAGCATGC CATGAAGGGT 
GTGATCCGAG TGAAGTTTGT CAACGACCTC GGGGTGGACG AGGCTGGGAT TGACCAGGAT 
GGTGTCTTCA AGGAGTTTCT GGAAGAGATC ATCAAGAGGG TGTTTGACCC CGCACTCAAC 
CTGTTCAAGA CAACCAGCGG GGATGAGCGG CTGTACCCAT CACCCACGTC CTACATTCAC 
GAGAATTACC TGCAGCTCTT CGAGTTTGTG GGGAAGATGC TGGGAAAGGC AGTGTATGAG 
GGGATCGTGG TAGATGTGCC CTTTGCCTCC TTCTTCCTGA GCCAGATGCT CGGGCACCAC 
CACAGCGTCT TCTACAGCTC CGTGGATGAG CTTCCCTCCC TGGACTCGGA GTTTTATAAA 
AACCTCACCT CCATCAAGCG CTACGATGGA GACATCGCAG ACCTCGGGCT GACGCTGTCT 
TACGATGAAG ACGTCATGGG TCAGCTCGTC TGCCACGAAC TGGTTCCCGG AGGGAAGACC 
ATCCCGGTTA CAGATGAGAA CAAAATTAGC TACATCCATC TGATGGCGCA TTTCCGAATG 
CACACTCAAA TTAAAAACCA GACAGCTGCT CTTATCAGTG GCTTCCGTTC CATCATCAAG 
CCCGAGTGGA TCCGAATGTT CTCTACCCCT GAGCTTCAGC GCCTCATCTC CGGGGACAAC 
GCTGAGATAG ATCTGGAAGA CTTAAAGAAG CACACAGTGT ACTACGGTGG CTTTCATGGG 
AGCCACAGGG TCATCATCTG GCTCTGGGAT ATCCTGGCTT CCGACTTCAC GCCTGAAGAA 
AGAGCCATGT TCCTGAAGTT TGTGACCAGC TGCTCCCGGC CCCCGCTCCT AGGATTCGCC 
TACCTCAAGC CTCCGTTCTC CATCCGCTGT GTGGAAGTAT CAGATGACCA GGACACTGGG 
GATACCCTGG GCAGCGTCCT CAGGGGCTTC TTCACCATCC GCAAGAGGGA GCCAGGGGGG 
CGTCTGCCCA CATCTTCCAC TTGCTTCAAC CTGCTTAAGC TGCCCAACTA CAGCAAGAAG 
AGCGTACTGC GAGAGAAACT ACGCTATGCC ATCAGCATGA ACACGGGCTT TGAGCTCTCC 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006530189.1
CDS	514..3576
Translation	MVHRQSPAGTRREAGAEGAGALSGDDPGPSPQFPLPEATAQRHQRLEKLCRSILNSMDAENEPKVWYVSLALSKDLTLLWIKQIKSILWHCCELLGQLKPEILQDSRLITLYLTMLVTFTDTSTWKILRGKGESLRPALNHICANIMGHLNQRGLYSVLQVLLTRGLARPRPCLSKGMLTAAFSLALRPVVAAQFSDNLMRPFIIHVMSVPALVAHLSTVAPERLGVLESHDMLRKFIVFLRDRDRCRDACESLEGCHTLCLMGNLLHLGSLSLRLLEEEMDGFVSALTQMLCYCQKYVAQKKSNLTHWHPVLGWFSQPVDYGLNDSMYLITKQLQFLWAVPLIRILFSDILSRKLLEHAEPAPVQPQPSSPQTVLPVKSLLKRAFQKSASVRNILRPVGGRRVDSAEVRKVCNICVLYQTSLTTLTQIRLQILTGLTYLDDLLPKLWAFICELGPHGGLKLFLECLNNDTGESKQLLAMLMLFCDCSRHLITILDDIEVYEEQISFKLEELVTISSFLNSFVFKMIWDGIVENAKGETLELFQSVHGWLMVLYERDCRRRFAPEDHWLRRDLKPGVLFQELDKDRRRAQLVLQHIPHVVPHKNRVLLFRNMVIKEKEKLGLVETSSASPHVTHITIRRSRMLEDGYEQLRQLSQHAMKGVIRVKFVNDLGVDEAGIDQDGVFKEFLEEIIKRVFDPALNLFKTTSGDERLYPSPTSYIHENYLQLFEFVGKMLGKAVYEGIVVDVPFASFFLSQMLGHHHSVFYSSVDELPSLDSEFYKNLTSIKRYDGDIADLGLTLSYDEDVMGQLVCHELVPGGKTIPVTDENKISYIHLMAHFRMHTQIKNQTAALISGFRSIIKPEWIRMFSTPELQRLISGDNAEIDLEDLKKHTVYYGGFHGSHRVIIWLWDILASDFTPEERAMFLKFVTSCSRPPLLGFAYLKPPFSIRCVEVSDDQDTGDTLGSVLRGFFTIRKREPGGRLPTSSTCFNLLKLPNYSKKSVLREKLRYAISMNTGFELS

Target ORF information:

RefSeq Version	XM_006530126.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus ubiquitin protein ligase E3B (Ube3b), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006530126.2

ORF Insert Sequence:

ATGGTTCATC GACAGAGCCC GGCAGGCACG AGAAGAGAGG CTGGTGCAGA AGGAGCGGGA 
GCGCTCAGCG GTGACGATCC AGGCCCTAGT CCGCAGTTTC CTCTGCCGGA GGCGACTGCA 
CAGAGACATC AGAGACTGGA GAAGTTGTGT CGAAGCATCC TGAACAGCAT GGATGCCGAG 
AATGAGCCAA AGGTGTGGTA CGTGTCCCTG GCTCTCTCCA AGGACCTCAC GCTGCTGTGG 
ATTAAGCAGA TCAAGAGCAT CCTGTGGCAC TGCTGTGAGC TTCTGGGGCA GCTCAAGCCA 
GAGATCTTAC AGGACTCCAG GCTCATCACG CTGTACCTCA CCATGCTTGT GACCTTCACA 
GACACTTCAA CATGGAAGAT TCTCCGGGGA AAAGGTGAGA GCCTTCGGCC AGCCCTGAAC 
CACATTTGCG CAAATATAAT GGGCCATCTC AACCAGCGTG GACTTTACTC TGTATTGCAG 
GTACTGCTGA CCCGAGGCCT GGCCAGGCCC CGGCCCTGTC TGTCCAAAGG CATGTTAACA 
GCAGCCTTCT CTCTGGCCCT GCGCCCTGTG GTTGCTGCGC AGTTCTCAGA CAATCTTATG 
CGACCATTCA TCATCCACGT CATGTCGGTA CCTGCTCTCG TGGCTCATCT CAGCACCGTG 
GCCCCTGAGC GCCTCGGCGT TTTAGAGTCC CATGACATGC TTCGTAAATT CATCGTCTTC 
CTGAGAGATA GAGACCGATG CCGCGATGCT TGTGAGAGTT TAGAAGGCTG CCATACCCTG 
TGTCTCATGG GCAATCTCCT GCACTTGGGC TCCCTCAGCC TCAGGCTGTT AGAGGAAGAG 
ATGGATGGGT TTGTGAGCGC GCTCACGCAG ATGCTGTGCT ACTGTCAGAA GTACGTGGCT 
CAGAAGAAGT CCAACCTGAC CCACTGGCAC CCTGTGCTCG GCTGGTTTTC CCAACCTGTG 
GATTACGGCC TCAATGATTC CATGTACCTG ATCACCAAGC AGCTGCAGTT CCTGTGGGCG 
GTGCCTCTCA TCCGCATTCT CTTCAGTGAC ATCCTGAGCA GGAAGCTGCT GGAGCATGCA 
GAGCCGGCCC CGGTGCAACC GCAGCCGTCC TCACCGCAGA CGGTGCTCCC CGTGAAGAGC 
CTCCTCAAGC GTGCGTTTCA GAAGTCGGCT TCGGTTCGGA ACATCCTCAG GCCTGTTGGG 
GGCCGCCGTG TCGACTCCGC TGAGGTGCGG AAGGTCTGCA ACATTTGCGT GCTCTACCAG 
ACCTCGCTGA CGACCCTCAC CCAGATCCGC CTGCAGATCC TCACAGGTCT TACGTACCTG 
GATGACCTGC TGCCCAAGCT CTGGGCGTTT ATCTGTGAGC TGGGGCCCCA CGGCGGGCTC 
AAGCTCTTCC TGGAGTGCCT GAACAATGAC ACTGGAGAGT CCAAGCAGCT CCTGGCCATG 
CTGATGCTCT TCTGTGACTG TTCCCGGCAC CTCATCACCA TCCTCGATGA CATTGAAGTT 
TATGAAGAAC AGATCTCCTT CAAGCTGGAA GAGCTGGTCA CCATCTCCTC GTTCCTCAAT 
TCCTTCGTGT TTAAGATGAT CTGGGACGGA ATTGTAGAGA ATGCCAAGGG TGAGACCTTG 
GAGCTGTTCC AGTCCGTCCA TGGCTGGCTG ATGGTGCTGT ATGAGCGAGA CTGCCGCCGG 
CGCTTTGCTC CGGAGGACCA CTGGCTACGG AGGGACCTCA AGCCTGGTGT GCTCTTCCAG 
GAGCTCGACA AGGACCGAAG GAGGGCTCAG CTGGTCCTGC AGCACATCCC CCACGTCGTG 
CCTCACAAGA ACAGAGTTCT TCTGTTCCGG AACATGGTTA TCAAGGAGAA AGAGAAGCTG 
GGGCTTGTGG AGACCAGCTC TGCCTCTCCT CACGTCACCC ACATCACCAT CCGAAGGTCC 
CGGATGTTGG AGGACGGCTA CGAACAGCTC CGGCAGCTTT CCCAGCATGC CATGAAGGGT 
GTGATCCGAG TGAAGTTTGT CAACGACCTC GGGGTGGACG AGGCTGGGAT TGACCAGGAT 
GGTGTCTTCA AGGAGTTTCT GGAAGAGATC ATCAAGAGGG TGTTTGACCC CGCACTCAAC 
CTGTTCAAGA CAACCAGCGG GGATGAGCGG CTGTACCCAT CACCCACGTC CTACATTCAC 
GAGAATTACC TGCAGCTCTT CGAGTTTGTG GGGAAGATGC TGGGAAAGGC AGTGTATGAG 
GGGATCGTGG TAGATGTGCC CTTTGCCTCC TTCTTCCTGA GCCAGATGCT CGGGCACCAC 
CACAGCGTCT TCTACAGCTC CGTGGATGAG CTTCCCTCCC TGGACTCGGA GTTTTATAAA 
AACCTCACCT CCATCAAGCG CTACGATGGA GACATCGCAG ACCTCGGGCT GACGCTGTCT 
TACGATGAAG ACGTCATGGG TCAGCTCGTC TGCCACGAAC TGGTTCCCGG AGGGAAGACC 
ATCCCGGTTA CAGATGAGAA CAAAATTAGC TACATCCATC TGATGGCGCA TTTCCGAATG 
CACACTCAAA TTAAAAACCA GACAGCTGCT CTTATCAGTG GCTTCCGTTC CATCATCAAG 
CCCGAGTGGA TCCGAATGTT CTCTACCCCT GAGCTTCAGC GCCTCATCTC CGGGGACAAC 
GCTGAGATAG ATCTGGAAGA CTTAAAGAAG CACACAGTGT ACTACGGTGG CTTTCATGGG 
AGCCACAGGG TCATCATCTG GCTCTGGGAT ATCCTGGCTT CCGACTTCAC GCCTGAAGAA 
AGAGCCATGT TCCTGAAGTT TGTGACCAGC TGCTCCCGGC CCCCGCTCCT AGGATTCGCC 
TACCTCAAGC CTCCGTTCTC CATCCGCTGT GTGGAAGTAT CAGATGACCA GGACACTGGG 
GATACCCTGG GCAGCGTCCT CAGGGGCTTC TTCACCATCC GCAAGAGGGA GCCAGGGGGG 
CGTCTGCCCA CATCTTCCAC TTGCTTCAAC CTGCTTAAGC TGCCCAACTA CAGCAAGAAG 
AGCGTACTGC GAGAGAAACT ACGCTATGCC ATCAGCATGA ACACGGGCTT TGAGCTCTCC 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Deficiency for the ubiquitin ligase UBE3B in a blepharophimosis-ptosis-intellectual-disability syndrome.
American journal of human genetics91(6)998-1010(2012 Dec)
Basel-Vanagaite L,Dallapiccola B,Ramirez-Solis R,Segref A,Thiele H,Edwards A,Arends MJ,Mir? X,White JK,D?sir J,Abramowicz M,Dentici ML,Lepri F,Hofmann K,Har-Zahav A,Ryder E,Karp NA,Estabel J,Gerdin AK,Podrini C,Ingham NJ,Altm?ller J,N?rnberg G,Frommolt P,Abdelhak S,Pasmanik-Chor M,Konen O,Kelley RI,Shohat M,N?rnberg P,Flint J,Steel KP,Hoppe T,Kubisch C,Adams DJ,Borck G

Characterization of the human UBE3B gene: structure, expression, evolution, and alternative splicing.
Genomics82(2)143-52(2003 Aug)
Gong TW,Huang L,Warner SJ,Lomax MI

Differential display and gene arrays to examine auditory plasticity.
Hearing research147(1-2)293-302(2000 Sep)
Lomax MI,Huang L,Cho Y,Gong TL,Altschuler RA

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UBE3B cDNA ORF clone, Mus musculus(House mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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