Alox8 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Alox8
Entrez Gene ID	11688
Full Name	arachidonate 8-lipoxygenase
Synonyms	15-LOX-2,15-LOX-B,8-LOX,8S-LOX,Alox15b
General protein information	Preferred Names arachidonate 8-lipoxygenase Names arachidonate 8S-lipoxygenase 15-lipoxygenase 2 8S-lipoxygenase arachidonate 15-lipoxygenase B arachidonate 15-lipoxygenase type II arachidonate 15-lipoxygenase, second type
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 11 Map Location: 11\|11 B3
Summary	This gene belongs to the lipoxygenase (LOX) gene family whose members encode enzymes that catalyze the addition of molecular oxygen to polyunsaturated fatty acids (PUFAs) to yield fatty acid hydroperoxides. The encoded enzyme preferentially metabolizes arachidonic acid to yield 8-hydroxyeicosatetraenoic acid (8-HETE), while metabolizing linoleic acid less efficiently. The gene may also function as a tumor suppressor. This gene is located in a cluster of related genes that spans approximately 75 kilobases on chromosome 11. [provided by RefSeq, Jan 2013].

mRNA and Protein(s)

mRNA	Protein	Name
NM_009661.4	NP_033791.1	arachidonate 8S-lipoxygenase

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	ALOX15B	XP_511865.2
Gallus gallus (chicken)	ALOXE3	XP_423676.4
Rattus norvegicus (Norway rat)	Alox15b	NP_695213.1
	ALOX15B	XP_001111890.1
Canis lupus familiaris (dog)	ALOX15B	XP_546603.1
Bos taurus (cattle)	ALOX15B	NP_001192632.1
Mus musculus (house mouse)	Alox8	NP_033791.1
Homo sapiens (human)	ALOX15B	NP_001132.2

Gene Ontology
Bibliography

Related articles in PubMed

Membrane-dependent Activities of Human 15-LOX-2 and Its Murine Counterpart: IMPLICATIONS FOR MURINE MODELS OF ATHEROSCLEROSIS.
Bender G, Schexnaydre EE, Murphy RC, Uhlson C, Newcomer ME
The Journal of biological chemistry291(37)19413-24(2016 09)

The role of group IIF-secreted phospholipase A2 in epidermal homeostasis and hyperplasia.
Yamamoto K, Miki Y, Sato M, Taketomi Y, Nishito Y, Taya C, Muramatsu K, Ikeda K, Nakanishi H, Taguchi R, Kambe N, Kabashima K, Lambeau G, Gelb MH, Murakami M
The Journal of experimental medicine212(11)1901-19(2015 Oct)

Expression of inactive glutathione peroxidase 4 leads to embryonic lethality, and inactivation of the Alox15 gene does not rescue such knock-in mice.
Br?tsch SH, Wang CC, Li L, Stender H, Neziroglu N, Richter C, Kuhn H, Borchert A
Antioxidants & redox signaling22(4)281-93(2015 Feb)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

15-LOX-2 is distributed at the plasma membrane when cells are stimulated by the addition Ca(2+) ionophore and that cellular localization is dependent upon the presence of a putative membrane insertion loop.
Title: Membrane-dependent Activities of Human 15-LOX-2 and Its Murine Counterpart: IMPLICATIONS FOR MURINE MODELS OF ATHEROSCLEROSIS.

Knockdown of mouse Alox15b in vivo decreased plaque lipid content and markers of inflammation.
Title: Arachidonate 15-lipoxygenase type B knockdown leads to reduced lipid accumulation and inflammation in atherosclerosis.

15-LOX2 expression in mouse prostate leads to hyperplasia and also induces cell senescence.
Title: Transgenic expression of 15-lipoxygenase 2 (15-LOX2) in mouse prostate leads to hyperplasia and cell senescence.

The reaction specificities of wild-type vertebrate lipoxygenase isoforms are largely resistant towards pH alterations.
Title: Structural basis for pH-dependent alterations of reaction specificity of vertebrate lipoxygenase isoforms.

Expression of 8S-LOX and 15S-LOX-2 suppresses CRD-BP/IMP-1 expression, resulting in inhibition of human prostate carcinoma PC-3 cell proliferation.
Title: Tumor-suppressive lipoxygenases inhibit the expression of c-myc mRNA coding region determinant-binding protein/insulin-like growth factor II mRNA-binding protein 1 in human prostate carcinoma PC-3 cells.

The following Alox8 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Alox8 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu04602	NM_009661.4 Latest version!	Mus musculus arachidonate 8-lipoxygenase (Alox8), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$391.30 $559.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Alox8 ( NM_009661.4 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_033791.1 Mus musculus arachidonate 8-lipoxygenase (Alox8), mRNA.

Price & Availability↑

CloneID	OMu04602
Clone ID Related Accession (Same CDS sequence)	NM_009661.4
Accession Version	NM_009661.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2034bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-24
Organism	Mus musculus(house mouse)
Product	arachidonate 8S-lipoxygenase
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL645527.20 and AK028724.1. On Jan 26, 2013 this sequence version replaced NM_009661.3. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK028724.1, Y14696.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164136 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: full length.

ATGGCGAAAT GCAGGGTGAG AGTATCCACG GGGGAAGCCT GTGGGGCTGG CACATGGGAC 
AAAGTGTCTG TCAGCATCGT GGGAACCCAC GGAGAGAGCC CCTTAGTACC TCTGGACCAT 
CTGGGCAAGG AGTTCAGCGC CGGTGCTGAA GAAGACTTCG AGGTGACGCT TCCCCAGGAC 
GTAGGCACTG TGCTGATGCT GCGAGTCCAC AAAGCACCCC CGGAAGTGTC CCTCCCGCTT 
ATGTCTTTCC GTTCTGATGC CTGGTTCTGC CGCTGGTTCG AGCTGGAGTG GCTACCTGGG 
GCTGCACTCC ACTTCCCCTG TTATCAGTGG CTGGAAGGGG CGGGGGAGCT GGTGCTGAGA 
GAGGGAGCAG CAAAGGTGTC CTGGCAAGAC CATCACCCTA CACTGCAGGA TCAGCGCCAG 
AAGGAGCTTG AGTCCAGGCA GAAGATGTAC AGCTGGAAGA CTTACATTGA AGGTTGGCCT 
CGCTGCCTTG ACCACGAGAC TGTGAAAGAC TTGGACCTCA ACATCAAGTA CTCTGCGATG 
AAGAATGCCA AACTCTTCTT TAAAGCCCAC TCCGCGTATA CGGAGCTGAA AGTCAAAGGG 
CTCCTGGACC GCACAGGACT CTGGAGGAGT CTGAGGGAGA TGAGAAGGCT GTTTAACTTC 
CGCAAGACTC CAGCAGCAGA GTATGTGTTT GCACACTGGC AGGAAGATGC CTTCTTCGCC 
TCCCAGTTCC TAAATGGCAT CAACCCGGTC CTGATTCGCC GCTGTCACAG TCTCCCAAAC 
AACTTCCCGG TCACTGATGA AATGGTGGCC CCAGTGCTGG GCCCTGGAAC CAGTCTGCAG 
GCTGAGTTGG AGAAGGGCTC CCTGTTCTTG GTGGATCATG GCATTCTTTC TGGAGTCCAC 
ACCAACATCC TCAATGGAAA GCCTCAGTTC TCTGCAGCCC CGATGACCCT GTTACACCAG 
AGCTCAGGGT CCGGACCCCT GCTTCCCATT GCCATCCAGC TCAAACAGAC TCCCGGGCCA 
GACAACCCCA TCTTCCTGCC CAGCGATGAC ACGTGGGACT GGTTGCTGGC CAAGACCTGG 
GTTCGCAATT CTGAGTTTTA CATCCATGAG GCTGTCACAC ATCTGCTGCA TGCCCATCTG 
ATTCCAGAAG TCTTTGCCTT GGCCACATTA CGTCAGCTGC CTAGGTGTCA CCCTCTCTTC 
AAGCTATTGA TTCCTCACAT TCGGTACACA CTGCACATCA ACACGCTTGC CCGGGAGCTG 
CTCGTTGCCC CTGGGAAGTT GATAGACAAG TCCACAGGCC TTGGCACTGG GGGATTCTCT 
GACCTGATAA AGAGAAACAT GGAGCAGCTG AACTACTCTG TCCTGTGTCT CCCTGAAGAT 
ATCCGAGCCC GAGGTGTGGA AGACATCCCA GGCTACTATT ACCGAGATGA TGGGATGCAG 
ATCTGGGGGG CAATAAAGAG CTTTGTCTCT GAAATAGTCA GCATCTACTA TCCAAGTGAC 
ACATCCGTCC AAGATGACCA AGAGCTCCAG GCCTGGGTGA GGGAGATCTT CTCTGAGGGC 
TTCCTCGGCC GAGAAAGCTC AGGTATGCCC TCCTTGTTGG ATACCCGGGA AGCCCTGGTC 
CAGTATATCA CCATGGTGAT ATTCACCTGC TCAGCCAAGC ATGCAGCTGT CAGTTCAGGC 
CAGTTCGACT CTTGTGTTTG GATGCCCAAT CTGCCACCTA CCATGCAGCT ACCACCACCT 
ACTTCCAAAG GCCAGGCCCG GCCTGAGAGT TTCATAGCCA CGCTCCCAGC AGTTAATTCG 
TCAAGTTATC ACATCATTGC TCTCTGGCTG CTAAGCGCAG AACCTGGGGA CCAAAGGCCC 
CTGGGCCACT ATCCAGATGA ACACTTCACA GAGGATGCCC CCCGGCGAAG CGTGGCTGCC 
TTCCAGAGAA AGCTGATCCA GATCTCCAAG GGCATCAGGG AGAGGAACCG AGGCCTGGCA 
CTGCCCTACA CCTACCTGGA TCCTCCCCTC ATTGAGAACA GTGTCTCCAT CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_033791.1
CDS	35..2068
Translation	MAKCRVRVSTGEACGAGTWDKVSVSIVGTHGESPLVPLDHLGKEFSAGAEEDFEVTLPQDVGTVLMLRVHKAPPEVSLPLMSFRSDAWFCRWFELEWLPGAALHFPCYQWLEGAGELVLREGAAKVSWQDHHPTLQDQRQKELESRQKMYSWKTYIEGWPRCLDHETVKDLDLNIKYSAMKNAKLFFKAHSAYTELKVKGLLDRTGLWRSLREMRRLFNFRKTPAAEYVFAHWQEDAFFASQFLNGINPVLIRRCHSLPNNFPVTDEMVAPVLGPGTSLQAELEKGSLFLVDHGILSGVHTNILNGKPQFSAAPMTLLHQSSGSGPLLPIAIQLKQTPGPDNPIFLPSDDTWDWLLAKTWVRNSEFYIHEAVTHLLHAHLIPEVFALATLRQLPRCHPLFKLLIPHIRYTLHINTLARELLVAPGKLIDKSTGLGTGGFSDLIKRNMEQLNYSVLCLPEDIRARGVEDIPGYYYRDDGMQIWGAIKSFVSEIVSIYYPSDTSVQDDQELQAWVREIFSEGFLGRESSGMPSLLDTREALVQYITMVIFTCSAKHAAVSSGQFDSCVWMPNLPPTMQLPPPTSKGQARPESFIATLPAVNSSSYHIIALWLLSAEPGDQRPLGHYPDEHFTEDAPRRSVAAFQRKLIQISKGIRERNRGLALPYTYLDPPLIENSVSI

Target ORF information:

RefSeq Version	NM_009661.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus arachidonate 8-lipoxygenase (Alox8), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_009661.4

ORF Insert Sequence:

ATGGCGAAAT GCAGGGTGAG AGTATCCACG GGGGAAGCCT GTGGGGCTGG CACATGGGAC 
AAAGTGTCTG TCAGCATCGT GGGAACCCAC GGAGAGAGCC CCTTAGTACC TCTGGACCAT 
CTGGGCAAGG AGTTCAGCGC CGGTGCTGAA GAAGACTTCG AGGTGACGCT TCCCCAGGAC 
GTAGGCACTG TGCTGATGCT GCGAGTCCAC AAAGCACCCC CGGAAGTGTC CCTCCCGCTT 
ATGTCTTTCC GTTCTGATGC CTGGTTCTGC CGCTGGTTCG AGCTGGAGTG GCTACCTGGG 
GCTGCACTCC ACTTCCCCTG TTATCAGTGG CTGGAAGGGG CGGGGGAGCT GGTGCTGAGA 
GAGGGAGCAG CAAAGGTGTC CTGGCAAGAC CATCACCCTA CACTGCAGGA TCAGCGCCAG 
AAGGAGCTTG AGTCCAGGCA GAAGATGTAC AGCTGGAAGA CTTACATTGA AGGTTGGCCT 
CGCTGCCTTG ACCACGAGAC TGTGAAAGAC TTGGACCTCA ACATCAAGTA CTCTGCGATG 
AAGAATGCCA AACTCTTCTT TAAAGCCCAC TCCGCGTATA CGGAGCTGAA AGTCAAAGGG 
CTCCTGGACC GCACAGGACT CTGGAGGAGT CTGAGGGAGA TGAGAAGGCT GTTTAACTTC 
CGCAAGACTC CAGCAGCAGA GTATGTGTTT GCACACTGGC AGGAAGATGC CTTCTTCGCC 
TCCCAGTTCC TAAATGGCAT CAACCCGGTC CTGATTCGCC GCTGTCACAG TCTCCCAAAC 
AACTTCCCGG TCACTGATGA AATGGTGGCC CCAGTGCTGG GCCCTGGAAC CAGTCTGCAG 
GCTGAGTTGG AGAAGGGCTC CCTGTTCTTG GTGGATCATG GCATTCTTTC TGGAGTCCAC 
ACCAACATCC TCAATGGAAA GCCTCAGTTC TCTGCAGCCC CGATGACCCT GTTACACCAG 
AGCTCAGGGT CCGGACCCCT GCTTCCCATT GCCATCCAGC TCAAACAGAC TCCCGGGCCA 
GACAACCCCA TCTTCCTGCC CAGCGATGAC ACGTGGGACT GGTTGCTGGC CAAGACCTGG 
GTTCGCAATT CTGAGTTTTA CATCCATGAG GCTGTCACAC ATCTGCTGCA TGCCCATCTG 
ATTCCAGAAG TCTTTGCCTT GGCCACATTA CGTCAGCTGC CTAGGTGTCA CCCTCTCTTC 
AAGCTATTGA TTCCTCACAT TCGGTACACA CTGCACATCA ACACGCTTGC CCGGGAGCTG 
CTCGTTGCCC CTGGGAAGTT GATAGACAAG TCCACAGGCC TTGGCACTGG GGGATTCTCT 
GACCTGATAA AGAGAAACAT GGAGCAGCTG AACTACTCTG TCCTGTGTCT CCCTGAAGAT 
ATCCGAGCCC GAGGTGTGGA AGACATCCCA GGCTACTATT ACCGAGATGA TGGGATGCAG 
ATCTGGGGGG CAATAAAGAG CTTTGTCTCT GAAATAGTCA GCATCTACTA TCCAAGTGAC 
ACATCCGTCC AAGATGACCA AGAGCTCCAG GCCTGGGTGA GGGAGATCTT CTCTGAGGGC 
TTCCTCGGCC GAGAAAGCTC AGGTATGCCC TCCTTGTTGG ATACCCGGGA AGCCCTGGTC 
CAGTATATCA CCATGGTGAT ATTCACCTGC TCAGCCAAGC ATGCAGCTGT CAGTTCAGGC 
CAGTTCGACT CTTGTGTTTG GATGCCCAAT CTGCCACCTA CCATGCAGCT ACCACCACCT 
ACTTCCAAAG GCCAGGCCCG GCCTGAGAGT TTCATAGCCA CGCTCCCAGC AGTTAATTCG 
TCAAGTTATC ACATCATTGC TCTCTGGCTG CTAAGCGCAG AACCTGGGGA CCAAAGGCCC 
CTGGGCCACT ATCCAGATGA ACACTTCACA GAGGATGCCC CCCGGCGAAG CGTGGCTGCC 
TTCCAGAGAA AGCTGATCCA GATCTCCAAG GGCATCAGGG AGAGGAACCG AGGCCTGGCA 
CTGCCCTACA CCTACCTGGA TCCTCCCCTC ATTGAGAACA GTGTCTCCAT CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Membrane-dependent Activities of Human 15-LOX-2 and Its Murine Counterpart: IMPLICATIONS FOR MURINE MODELS OF ATHEROSCLEROSIS.
The Journal of biological chemistry291(37)19413-24(2016 09)
Bender G,Schexnaydre EE,Murphy RC,Uhlson C,Newcomer ME

The role of group IIF-secreted phospholipase A2 in epidermal homeostasis and hyperplasia.
The Journal of experimental medicine212(11)1901-19(2015 Oct)
Yamamoto K,Miki Y,Sato M,Taketomi Y,Nishito Y,Taya C,Muramatsu K,Ikeda K,Nakanishi H,Taguchi R,Kambe N,Kabashima K,Lambeau G,Gelb MH,Murakami M

Expression of inactive glutathione peroxidase 4 leads to embryonic lethality, and inactivation of the Alox15 gene does not rescue such knock-in mice.
Antioxidants & redox signaling22(4)281-93(2015 Feb)
Br?tsch SH,Wang CC,Li L,Stender H,Neziroglu N,Richter C,Kuhn H,Borchert A

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Alox8 cDNA ORF clone, Mus musculus(house mouse)

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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