Alox12b cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Alox12b
Entrez Gene ID	11686
Full Name	arachidonate 12-lipoxygenase, 12R type
Synonyms	12R-LOX,Aloxe2,e-LOX2
General protein information	Preferred Names arachidonate 12-lipoxygenase, 12R type Names arachidonate 12-lipoxygenase, 12R-type 12R-lipoxygenase epidermis-type lipoxygenase 12 epidermis-type lipoxygenase 2
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 11 Map Location: 11 B3\|11 42.38 cM
Summary	This gene encodes an enzyme involved in the conversion of arachidonic acid to 12R-hydroxyeicosatetraenoic acid. Mutations in this gene can prevent the formation of the epidermal permeability barrier and cause an ichthyosiform phenotype. [provided by RefSeq, Sep 2015].

mRNA and Protein(s)

mRNA	Protein	Name
NM_009659.2	NP_033789.1	arachidonate 12-lipoxygenase, 12R-type

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Alox12b	NP_033789.1
Homo sapiens (human)	ALOX12B	NP_001130.1
Pan troglodytes (chimpanzee)	ALOX12B	XP_511864.2
Bos taurus (cattle)	ALOX12B	NP_001178967.1
Canis lupus familiaris (dog)	ALOX12B	XP_546604.2
Rattus norvegicus (Norway rat)	Alox12b	NP_001034466.1

Gene Ontology
Bibliography

Related articles in PubMed

PNPLA1 defects in patients with autosomal recessive congenital ichthyosis and KO mice sustain PNPLA1 irreplaceable function in epidermal omega-O-acylceramide synthesis and skin permeability barrier.
Pichery M, Huchenq A, Sandhoff R, Severino-Freire M, Zaafouri S, Op?lka L, Levade T, Soldan V, Bertrand-Michel J, Lhuillier E, Serre G, Maruani A, Mazereeuw-Hautier J, Jonca N
Human molecular genetics26(10)1787-1800(2017 05)

The role of lipoxygenases in epidermis.
Krieg P, F?rstenberger G
Biochimica et biophysica acta1841(3)390-400(2014 Mar)

Aloxe3 knockout mice reveal a function of epidermal lipoxygenase-3 as hepoxilin synthase and its pivotal role in barrier formation.
Krieg P, Rosenberger S, de Juanes S, Latzko S, Hou J, Dick A, Kloz U, van der Hoeven F, Hausser I, Esposito I, Rauh M, Schneider H
The Journal of investigative dermatology133(1)172-80(2013 Jan)

Lipoxygenases mediate the effect of essential fatty acid in skin barrier formation: a proposed role in releasing omega-hydroxyceramide for construction of the corneocyte lipid envelope.
Zheng Y, Yin H, Boeglin WE, Elias PM, Crumrine D, Beier DR, Brash AR
The Journal of biological chemistry286(27)24046-56(2011 Jul)

Development of an ichthyosiform phenotype in Alox12b-deficient mouse skin transplants.
de Juanes S, Epp N, Latzko S, Neumann M, F?rstenberger G, Hausser I, Stark HJ, Krieg P
The Journal of investigative dermatology129(6)1429-36(2009 Jun)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Loss-of-function mutations in the LOX genes ALOX12B and ALOXE3 have been found to represent the second most common cause of autosomal recessive congenital ichthyosis. [review]
Title: The role of lipoxygenases in epidermis.

Alox12b-deficient mouse skin transplants have an ichthyosiform phenotype
Title: Development of an ichthyosiform phenotype in Alox12b-deficient mouse skin transplants.

The 12R-LOX-eLOX-3 pathway plays a key role in the process of epidermal barrier acquisition by affecting lipid metabolism, as well as protein processing.
Title: 12R-lipoxygenase deficiency disrupts epidermal barrier function.

The following Alox12b gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Alox12b cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu18349	NM_009659.2 Latest version!	Mus musculus arachidonate 12-lipoxygenase, 12R type (Alox12b), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$412.30 $589.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Alox12b ( NM_009659.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_033789.1 Mus musculus arachidonate 12-lipoxygenase, 12R type (Alox12b), mRNA.

Price & Availability↑

CloneID	OMu18349
Clone ID Related Accession (Same CDS sequence)	NM_009659.2
Accession Version	NM_009659.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2106bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-28
Organism	Mus musculus(house mouse)
Product	arachidonate 12-lipoxygenase, 12R-type
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL645527.20. On Nov 17, 2006 this sequence version replaced NM_009659.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: Y14334.1, BC113149.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849376, SAMN00849387 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCCACCT ATAAAGTCAA GGTGGCCACG GGCACTGACT TCTTTTCGGG GACGCTGGAT 
TCAATTTCAC TGACCATCGT GGGGACCCAA GGAGAGAGCC ATAAGCAGCG GTTGAACCAC 
TTTGGGAGAG ACTTTGCCAC GGGGGCGGTG GATGACTACA CGGTACAGTG CCAGCAGGAC 
CTGGGGGAGC TTATCATCAT CCGTCTGCAC AAGGAGCCTC ATTCCTTCCT AGCCAAGGAC 
CCCTGGTACT GCAACTATGT GCAGATCTGT GCTCCCGACT GCCGTGTCTA CCACTTCCCA 
GCTTACCAGT GGATGGATGG CTATGAGACC CTGGCACTCC GGGAGGCCAC AGGAAAGATA 
ACAGCAGACG ACACACTCCC TATCCTTTTG GAACACAGAC AAGAAGAGAT CAGAGCCAAG 
AAGGACTTCT ACCACTGGAG GGTCTTTGTT CCTGGCCTTC CCAACTATGT GGATATCCCC 
AGTTACCATC CTCCTCCCCG ACGGTGCCGC AATCCCAACC GGCCTGAGTG GGATGGTTAT 
ATTCCCGGAT TCCCAATTCT CATCAACATT AAGGCTACCA GGTTCTTAAA CTCAAATCTT 
CGTTTCTCCT TCGTCAAGAC AGCCTCCTTC TTCTACCGCC TAGGGCCCAT GGCGCTGGCC 
TTCAAACTGC GTGGCCTGGT GGATCGAAAA CGCTCCTGGA AGAGACTAAA GGACATTAAG 
AATATTTTCC CTGCTACCAA GTCTGTCGTC TCAGAGTATG TGGCCGAGCA CTGGACAGAG 
GACAGCTTCT TTGGGTACCA GTACCTTAAT GGTATTAACC CAGGCCTGAT CCGAAGATGT 
ACACAGATCC CAGACAAATT CCCAGTCACA GATGAAATGG TGGCCCCATT CCTGGGTGAA 
GGAACATGCC TGCAAGCAGA GCTGGAGAGG GGGAATATTT ACCTGGCAGA CTACCGCATC 
CTGGATGGCA TCCCCACCGT GGAGCTCAAT GGTCAGCAGC AGCATCACTG TGCCCCGATG 
TGCTTGCTGC ACTTTGGTCC TGATGGCAAC ATGATGCCCA TCGCCATTCA GCTCAGTCAG 
ACTCCTGGGC CTGATTGTCC TATCTTCCTG CCGAATGATT CTGAGTGGGA CTGGCTGTTG 
GCTAAAACGT GGGTGCGCTA CGCGGAGTTC TACAGCCACG AGGCCGTGGC ACACTTGCTG 
GAGAGCCACC TCATCGGGGA AGCTTTCTGC TTGGCCCTCC TGAGGAACCT GCCCATGTGC 
CACCCCCTGT ATAAGCTTCT CATCCCTCAC ACTCGGTACA ACGTCCAGAT CAATAGCATT 
GGGCGGGCCC TCCTTCTCAA CAAGGGAGGT CTCTCTGCCA GGGCCATGTC CCTGGGCCTA 
GAGGGCTTCG CACAGGTGAT GGTTCGGGGT CTGTCTGAGC TCACCTACAA AAGCCTCTGC 
ATTCCCAACG ACTTTGTGGA GCGCGGGGTC CAGGACCTGC CTGGGTATTA TTTCCGTGAT 
GACAGCCTGG CGGTGTGGTA CGCGATGGAG AGGTACGTGA CAGAGATCAT CACTTATTAT 
TACCCAAATG ACGCAGCCGT AGAGGGCGAC CCGGAACTGC AGTGCTGGGT ACAGGAGATA 
TTCAAGGAGT GTCTCCTGGG GCGTGAGAGC TCAGGCTTCC CCACCTGCTT GCGAACTATA 
CCTGAGCTGA TCGAATACGT CACAATGGTC ATGTACACCT GCTCGGCCAG GCATGCAGCT 
GTCAACTCAG GCCAGCTGGA GTACACCTCT TGGATGCCCA ACTTCCCATC ATCCATGAGG 
AACCCACCGA TGCAGACCAA GGGGCTAACT ACTCTGCAGA CATACATGGA CACTCTTCCG 
GACGTGAAGA CCACATGCAT TGTGCTCTTG GTGCTCTGGA CTCTCTGTCG GGAGCCTGAT 
GACAGGCGGC CGCTTGGCCA CTTCCCAGAC ATCCATTTTG TGGAGGAGGG TCCTCGGAGG 
AGTATTGAGG CTTTTCGTCA GAACCTCAAC CAGATCTCTC ACAACATCCG ACAGCGCAAT 
AAGTGCCTCA CCCTCCCGTA CTACTACCTG GACCCAGTGC TGATTGAGAA CAGTATTTCC 
ATCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_033789.1
CDS	176..2281
Translation	MATYKVKVATGTDFFSGTLDSISLTIVGTQGESHKQRLNHFGRDFATGAVDDYTVQCQQDLGELIIIRLHKEPHSFLAKDPWYCNYVQICAPDCRVYHFPAYQWMDGYETLALREATGKITADDTLPILLEHRQEEIRAKKDFYHWRVFVPGLPNYVDIPSYHPPPRRCRNPNRPEWDGYIPGFPILINIKATRFLNSNLRFSFVKTASFFYRLGPMALAFKLRGLVDRKRSWKRLKDIKNIFPATKSVVSEYVAEHWTEDSFFGYQYLNGINPGLIRRCTQIPDKFPVTDEMVAPFLGEGTCLQAELERGNIYLADYRILDGIPTVELNGQQQHHCAPMCLLHFGPDGNMMPIAIQLSQTPGPDCPIFLPNDSEWDWLLAKTWVRYAEFYSHEAVAHLLESHLIGEAFCLALLRNLPMCHPLYKLLIPHTRYNVQINSIGRALLLNKGGLSARAMSLGLEGFAQVMVRGLSELTYKSLCIPNDFVERGVQDLPGYYFRDDSLAVWYAMERYVTEIITYYYPNDAAVEGDPELQCWVQEIFKECLLGRESSGFPTCLRTIPELIEYVTMVMYTCSARHAAVNSGQLEYTSWMPNFPSSMRNPPMQTKGLTTLQTYMDTLPDVKTTCIVLLVLWTLCREPDDRRPLGHFPDIHFVEEGPRRSIEAFRQNLNQISHNIRQRNKCLTLPYYYLDPVLIENSISI

Target ORF information:

RefSeq Version	NM_009659.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus arachidonate 12-lipoxygenase, 12R type (Alox12b), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_009659.2

ORF Insert Sequence:

ATGGCCACCT ATAAAGTCAA GGTGGCCACG GGCACTGACT TCTTTTCGGG GACGCTGGAT 
TCAATTTCAC TGACCATCGT GGGGACCCAA GGAGAGAGCC ATAAGCAGCG GTTGAACCAC 
TTTGGGAGAG ACTTTGCCAC GGGGGCGGTG GATGACTACA CGGTACAGTG CCAGCAGGAC 
CTGGGGGAGC TTATCATCAT CCGTCTGCAC AAGGAGCCTC ATTCCTTCCT AGCCAAGGAC 
CCCTGGTACT GCAACTATGT GCAGATCTGT GCTCCCGACT GCCGTGTCTA CCACTTCCCA 
GCTTACCAGT GGATGGATGG CTATGAGACC CTGGCACTCC GGGAGGCCAC AGGAAAGATA 
ACAGCAGACG ACACACTCCC TATCCTTTTG GAACACAGAC AAGAAGAGAT CAGAGCCAAG 
AAGGACTTCT ACCACTGGAG GGTCTTTGTT CCTGGCCTTC CCAACTATGT GGATATCCCC 
AGTTACCATC CTCCTCCCCG ACGGTGCCGC AATCCCAACC GGCCTGAGTG GGATGGTTAT 
ATTCCCGGAT TCCCAATTCT CATCAACATT AAGGCTACCA GGTTCTTAAA CTCAAATCTT 
CGTTTCTCCT TCGTCAAGAC AGCCTCCTTC TTCTACCGCC TAGGGCCCAT GGCGCTGGCC 
TTCAAACTGC GTGGCCTGGT GGATCGAAAA CGCTCCTGGA AGAGACTAAA GGACATTAAG 
AATATTTTCC CTGCTACCAA GTCTGTCGTC TCAGAGTATG TGGCCGAGCA CTGGACAGAG 
GACAGCTTCT TTGGGTACCA GTACCTTAAT GGTATTAACC CAGGCCTGAT CCGAAGATGT 
ACACAGATCC CAGACAAATT CCCAGTCACA GATGAAATGG TGGCCCCATT CCTGGGTGAA 
GGAACATGCC TGCAAGCAGA GCTGGAGAGG GGGAATATTT ACCTGGCAGA CTACCGCATC 
CTGGATGGCA TCCCCACCGT GGAGCTCAAT GGTCAGCAGC AGCATCACTG TGCCCCGATG 
TGCTTGCTGC ACTTTGGTCC TGATGGCAAC ATGATGCCCA TCGCCATTCA GCTCAGTCAG 
ACTCCTGGGC CTGATTGTCC TATCTTCCTG CCGAATGATT CTGAGTGGGA CTGGCTGTTG 
GCTAAAACGT GGGTGCGCTA CGCGGAGTTC TACAGCCACG AGGCCGTGGC ACACTTGCTG 
GAGAGCCACC TCATCGGGGA AGCTTTCTGC TTGGCCCTCC TGAGGAACCT GCCCATGTGC 
CACCCCCTGT ATAAGCTTCT CATCCCTCAC ACTCGGTACA ACGTCCAGAT CAATAGCATT 
GGGCGGGCCC TCCTTCTCAA CAAGGGAGGT CTCTCTGCCA GGGCCATGTC CCTGGGCCTA 
GAGGGCTTCG CACAGGTGAT GGTTCGGGGT CTGTCTGAGC TCACCTACAA AAGCCTCTGC 
ATTCCCAACG ACTTTGTGGA GCGCGGGGTC CAGGACCTGC CTGGGTATTA TTTCCGTGAT 
GACAGCCTGG CGGTGTGGTA CGCGATGGAG AGGTACGTGA CAGAGATCAT CACTTATTAT 
TACCCAAATG ACGCAGCCGT AGAGGGCGAC CCGGAACTGC AGTGCTGGGT ACAGGAGATA 
TTCAAGGAGT GTCTCCTGGG GCGTGAGAGC TCAGGCTTCC CCACCTGCTT GCGAACTATA 
CCTGAGCTGA TCGAATACGT CACAATGGTC ATGTACACCT GCTCGGCCAG GCATGCAGCT 
GTCAACTCAG GCCAGCTGGA GTACACCTCT TGGATGCCCA ACTTCCCATC ATCCATGAGG 
AACCCACCGA TGCAGACCAA GGGGCTAACT ACTCTGCAGA CATACATGGA CACTCTTCCG 
GACGTGAAGA CCACATGCAT TGTGCTCTTG GTGCTCTGGA CTCTCTGTCG GGAGCCTGAT 
GACAGGCGGC CGCTTGGCCA CTTCCCAGAC ATCCATTTTG TGGAGGAGGG TCCTCGGAGG 
AGTATTGAGG CTTTTCGTCA GAACCTCAAC CAGATCTCTC ACAACATCCG ACAGCGCAAT 
AAGTGCCTCA CCCTCCCGTA CTACTACCTG GACCCAGTGC TGATTGAGAA CAGTATTTCC 
ATCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

PNPLA1 defects in patients with autosomal recessive congenital ichthyosis and KO mice sustain PNPLA1 irreplaceable function in epidermal omega-O-acylceramide synthesis and skin permeability barrier.
Human molecular genetics26(10)1787-1800(2017 05)
Pichery M,Huchenq A,Sandhoff R,Severino-Freire M,Zaafouri S,Op?lka L,Levade T,Soldan V,Bertrand-Michel J,Lhuillier E,Serre G,Maruani A,Mazereeuw-Hautier J,Jonca N

The role of lipoxygenases in epidermis.
Biochimica et biophysica acta1841(3)390-400(2014 Mar)
Krieg P,F?rstenberger G

Aloxe3 knockout mice reveal a function of epidermal lipoxygenase-3 as hepoxilin synthase and its pivotal role in barrier formation.
The Journal of investigative dermatology133(1)172-80(2013 Jan)
Krieg P,Rosenberger S,de Juanes S,Latzko S,Hou J,Dick A,Kloz U,van der Hoeven F,Hausser I,Esposito I,Rauh M,Schneider H

Lipoxygenases mediate the effect of essential fatty acid in skin barrier formation: a proposed role in releasing omega-hydroxyceramide for construction of the corneocyte lipid envelope.
The Journal of biological chemistry286(27)24046-56(2011 Jul)
Zheng Y,Yin H,Boeglin WE,Elias PM,Crumrine D,Beier DR,Brash AR

Development of an ichthyosiform phenotype in Alox12b-deficient mouse skin transplants.
The Journal of investigative dermatology129(6)1429-36(2009 Jun)
de Juanes S,Epp N,Latzko S,Neumann M,F?rstenberger G,Hausser I,Stark HJ,Krieg P

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Alox12b cDNA ORF clone, Mus musculus(house mouse)

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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