Add2 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Add2
Entrez Gene ID	11519
Full Name	adducin 2 (beta)
Synonyms	2900072M03Rik,add97
General protein information	Preferred Names adducin 2 (beta) Names beta-adducin erythrocyte adducin subunit beta snoRNA MBII-396
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 6 Map Location: 6 C3-D1\|6 37.55 cM
Summary	This gene encodes the beta subunit of the adducin family. Adducins, encoded by alpha, beta and gamma genes, are heteromeric proteins that crosslink actin filaments with spectrin at the cytoskeletal membrane. This protein, primarily found in the brain and hematopoietic cells, is regulated by phosphorylation and calmodulin interactions as it promotes spectrin assembly onto actin filaments, bundles actin and caps barbed ends of actin filaments. In mouse, deficiency of this gene can lead to mild hemolytic anemia and impaired synaptic plasticity. Mutations of this gene in mouse serve as a pathophysiological model for hereditary spherocytosis and hereditary elliptocytosis. Alternative splicing results in multiple transcript variants that encode different protein isoforms. [provided by RefSeq, Dec 2012].

mRNA and Protein(s)

mRNA	Protein	Name
NM_001271858.1	NP_001258787.1	beta-adducin isoform 1
NM_001271861.1	NP_001258790.1	beta-adducin isoform 2
NM_001271859.1	NP_001258788.1	beta-adducin isoform 1
NM_013458.5	NP_038486.2	beta-adducin isoform 1
NM_001271860.1	NP_001258789.1	beta-adducin isoform 2
NM_001271857.1	NP_001258786.1	beta-adducin isoform 1

Pathways from BioSystems

Homologies

Homo sapiens (human)	ADD2	NP_001171983.1
Mus musculus (house mouse)	Add2	NP_001258786.1
	ADD2	XP_002799318.1
Danio rerio (zebrafish)	add2	XP_698680.6
Pan troglodytes (chimpanzee)	ADD2	XP_003309148.1
Canis lupus familiaris (dog)	ADD2	XP_853066.1
Xenopus tropicalis (tropical clawed frog)	add2	NP_001096433.1
Bos taurus (cattle)	ADD2	NP_001092479.1
Rattus norvegicus (Norway rat)	Add2	NP_001103350.1

Gene Ontology
Bibliography

Related articles in PubMed

Sez6l2 regulates phosphorylation of ADD and neuritogenesis.
Yaguchi H, Yabe I, Takahashi H, Watanabe M, Nomura T, Kano T, Matsumoto M, Nakayama KI, Watanabe M, Hatakeyama S
Biochemical and biophysical research communications494(1-2)234-241(2017 12)

Circulating primitive erythroblasts establish a functional, protein 4.1R-dependent cytoskeletal network prior to enucleating.
Huang YS, Delgadillo LF, Cyr KH, Kingsley PD, An X, McGrath KE, Mohandas N, Conboy JG, Waugh RE, Wan J, Palis J
Scientific reports7(1)5164(2017 Jul)

Acute drug-induced spine changes in the nucleus accumbens are dependent on ?-adducin.
Engmann O, Giralt A, Girault JA
Neuropharmacology110(Pt A)333-342(2016 Nov)

Impaired synaptic plasticity and learning in mice lacking beta-adducin, an actin-regulating protein.
Rabenstein RL, Addy NA, Caldarone BJ, Asaka Y, Gruenbaum LM, Peters LL, Gilligan DM, Fitzsimonds RM, Picciotto MR
The Journal of neuroscience : the official journal of the Society for Neuroscience25(8)2138-45(2005 Feb)

The mouse adducin gene family: alternative splicing and chromosomal localization.
Suriyapperuma SP, Lozovatsky L, Ciciotte SL, Peters LL, Gilligan DM
Mammalian genome : official journal of the International Mammalian Genome Society11(1)16-23(2000 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Sez6l2 is one of the auxiliary subunits of the AMPA receptor and acts as a scaffolding protein to link GluR1 to ADD. Furthermore, Sez6l2 overexpression upregulates ADD phosphorylation, whereas siRNA-mediated downregulation of Sez612 prevents ADD phosphorylation, suggesting that Sez6l2 modulates AMPA-ADD signal transduction.
Title: Sez6l2 regulates phosphorylation of ADD and neuritogenesis.

Darpp32 interaction with beta-adducin may be the molecular pathway by which environmental changes may rapidly alter responsiveness of striatal neurons involved in the reward system.
Title: DARPP-32 interaction with adducin may mediate rapid environmental effects on striatal neurons.

Calcium plays a role in regulating the expression and function of beta-adducin to sustain normal organization of the spectrin-based cytoskeleton and the differentiation properties in keratinocytes through the calmodulin/EGFR/cadherin signaling pathway.
Title: ?-Adducin siRNA disruption of the spectrin-based cytoskeleton in differentiating keratinocytes prevented by calcium acting through calmodulin/epidermal growth factor receptor/cadherin pathway.

chorein interacts with beta-adducin and beta-actin.
Title: Chorein, the protein responsible for chorea-acanthocytosis, interacts with ?-adducin and ?-actin.

study identified novel long-distance upstream non-canonical polyadenylation-regulatory elements that enhance or silence 3'end processing of the Add2 PAS4
Title: Long-distance regulation of Add2 pre-mRNA3'end processing.

The following Add2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Add2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu09405	NM_013458.5 Latest version!	Mus musculus adducin 2 (beta) (Add2), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
OMu09405	NM_001271857.1 Latest version!	Mus musculus adducin 2 (beta) (Add2), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
OMu09405	NM_001271858.1 Latest version!	Mus musculus adducin 2 (beta) (Add2), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
OMu09405	NM_001271859.1 Latest version!	Mus musculus adducin 2 (beta) (Add2), transcript variant 4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
OMu07382	NM_001271861.1 Latest version!	Mus musculus adducin 2 (beta) (Add2), transcript variant 6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$307.30 $439.00
OMu07382	NM_001271860.1 Latest version!	Mus musculus adducin 2 (beta) (Add2), transcript variant 5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$307.30 $439.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Add2 ( NM_001271858.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001258787.1 Mus musculus adducin 2 (beta) (Add2), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OMu09405
Clone ID Related Accession (Same CDS sequence)	NM_001271858.1 , NM_001271859.1 , NM_013458.5 , NM_001271857.1
Accession Version	NM_001271858.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2178bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-24
Organism	Mus musculus(house mouse)
Product	beta-adducin isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BY146240.1, AK029488.1, AK147544.1, AK014496.1, CB722600.1, BU708888.1, CB524359.1, BU058008.1, BY712734.1, CB519116.1, BE951615.1 and AI835294.1. Transcript Variant: This variant (2) differs in the 5' UTR compared to variant 1. Variants 1, 2, 3 and 4 encode the same isoform (1, also known as beta-1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK029488.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN01164131 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAGCGAGG ACACGGTTCC CGAGGCGGCC TCCCCGCCAC CCTCTCAGGG GCAGCACTAC 
TTTGACCGGT TCTCTGAAGA TGATCCTGAG TACTTGCGAC TTCGCAACCG TGCAGCTGAC 
CTGCGACAGG ACTTCAACTT GATGGAGCAG AAGAAGAGGG TCACCATGAT CCTGCAGAGC 
CCTTCTTTCA GGGAGGAGCT GGAAGGCCTC ATCCAGGAGC AAATGAAGAA GGGCAACAAC 
TCCTCCAACA TCTGGGCCCT CCGACAGATC GCGGACTTCA TGGCCAGCAC CTCCCACGCA 
GTCTTCCCAG CTTCTTCCAT GAACTTCTCC ATGATGACAC CCATCAACGA CCTCCACACT 
GCCGACTCCC TGAACCTGGC CAAGGGGGAG AGGCTTATGC GGTGCAAGAT CAGCAGCGTC 
TACCGTCTCC TGGACCTCTA CGGCTGGGCG CAGCTCAGTG ACACCTACGT CACGCTGAGA 
GTGAGCAAGG AGCAGGACCA CTTCCTGATC AGCCCCAAGG GGGTTTCCTG CAGCGAGGTC 
ACAGCCTCCA GCCTGATTAA AGTGAACATT CTGGGAGAGG TGGTGGAGAA GGGCAGCAGT 
TGCTTCCCGG TGGACACCAC GGGCTTCAGT CTGCACTCAG CCATCTATGC CGCCAGGCCC 
GACGTGCGGT GTGCCATCCA CCTGCACACG CCTGCCACCG CAGCGGTGTC AGCTATGAAG 
TGCGGCCTCC TGCCGGTCTC CCATAATGCC CTGCTGGTGG GGGACATGGC CTACTATGAC 
TTCAATGGGG AAATGGAGCA GGAAGCTGAC CGAATCAACT TGCAGAAGTG CCTTGGACCC 
ACCTGCAAGA TTCTGGTGCT AAGAAACCAT GGCATGGTCG CCCTGGGTGA CACCGTGGAG 
GAAGCTTTCT ACAAGGTCTT CCATCTGCAG GCTGCGTGTG AGGTACAGGT GTCGGCTCTG 
TCCAGTGCTG GGGGCACTGA GAACCTCATC CTCTTGGAGC AAGAGAAACA CCGGCCGCAC 
GAGGTGGGCT CTGTGCAGTG GGCCGGCAGC ACCTTCGGGC CCATGCAGAA GAGCCGGCTG 
GGAGAGCATG AATTTGAAGC CCTCATGAGG ATGCTGGACA ATTTAGGCTA CAGAACAGGC 
TATACGTACC GCCACCCCTT TGTCCAAGAG AAAACCAAAC ACAAAAGTGA AGTGGAGATC 
CCAGCCACAG TCACTGCCTT TGTGTTTGAA GAGGATGGAG TCCCAGTCCC CGCCCTGCGC 
CAGCACGCCC AGAAGCAGCA GAAGGAAAAG ACCCGCTGGC TTAACACTCC CAACACCTAC 
CTGCGGGTGA ACGTGGCTGA CGAGGTGCAG AGGAACATGG GCAGTCCCCG ACCAAAGACC 
ACGTGGATGA AGGCTGATGA AGTGGAAAAG TCCAGCAGCG GCATGCCCAT ACGGATTGAA 
AACCCAAACC AATTTGTGCC TCTCTACACT GACCCCCAGG AAGTTCTGGA CATGAGGAAC 
AAGATTCGAG AGCAAAACCG ACAAGACATA AAGTCAGCCG GGCCTCAGTC TCAGCTCTTG 
GCCAGTGTCA TCGCAGAGAA GAGCCGGAGT CCGTCTACAG AGAGCCAGCT GATGTCCAAG 
GGCGATGCAG ATACCAAAGA TGAATCGGAG GAGACGGTGC CCAACCCCTT CAGCCAACTC 
ACTGACCAGG AGCTGGAGGA GTACAAGAAG GAGGTGGAAA GGAAGAAGCT AGAACAGGAG 
CAGGAAGGGG AGAAGGACAT AGCCACAGAG AAGCCTGGTT CACCTGTAAA GTCCACACCT 
GCATCCCCAG TGCAGAGCCC ATCAAAAGCC GGGACCAAGA GCCCAGCGGT CTCTCCTTCC 
AAGACTTCAG AGGATACCAA GAAGACAGAA GTCAGCGAAG CCAACACAGA GCCTGAGCCA 
GTGAAACCAG AAGGGCTGGT GGTGAATGGG AAGGAGGAGG AGCCGAGCGT AGAAGAGGCC 
CTCAGCAAAG GACTGGGCCA GATGACCACC AACGCTGACA CTGATGGCGA CAGCTACAAG 
GACAAAACCG AGTCAGTTAC CAGTGGCCCC TTGTCCCCAG AGGGCTCGCC CTCTAAGTCA 
CCCTCCAAAA AGAAAAAGAA ATTCCGAACC CCCTCGTTCC TGAAGAAAAG CAAGAAGAAG 
GAGAAGGTGG AATCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001258787.1
CDS	400..2577
Translation	MSEDTVPEAASPPPSQGQHYFDRFSEDDPEYLRLRNRAADLRQDFNLMEQKKRVTMILQSPSFREELEGLIQEQMKKGNNSSNIWALRQIADFMASTSHAVFPASSMNFSMMTPINDLHTADSLNLAKGERLMRCKISSVYRLLDLYGWAQLSDTYVTLRVSKEQDHFLISPKGVSCSEVTASSLIKVNILGEVVEKGSSCFPVDTTGFSLHSAIYAARPDVRCAIHLHTPATAAVSAMKCGLLPVSHNALLVGDMAYYDFNGEMEQEADRINLQKCLGPTCKILVLRNHGMVALGDTVEEAFYKVFHLQAACEVQVSALSSAGGTENLILLEQEKHRPHEVGSVQWAGSTFGPMQKSRLGEHEFEALMRMLDNLGYRTGYTYRHPFVQEKTKHKSEVEIPATVTAFVFEEDGVPVPALRQHAQKQQKEKTRWLNTPNTYLRVNVADEVQRNMGSPRPKTTWMKADEVEKSSSGMPIRIENPNQFVPLYTDPQEVLDMRNKIREQNRQDIKSAGPQSQLLASVIAEKSRSPSTESQLMSKGDADTKDESEETVPNPFSQLTDQELEEYKKEVERKKLEQEQEGEKDIATEKPGSPVKSTPASPVQSPSKAGTKSPAVSPSKTSEDTKKTEVSEANTEPEPVKPEGLVVNGKEEEPSVEEALSKGLGQMTTNADTDGDSYKDKTESVTSGPLSPEGSPSKSPSKKKKKFRTPSFLKKSKKKEKVES

Target ORF information:

RefSeq Version	NM_001271858.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus adducin 2 (beta) (Add2), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001271858.1

ORF Insert Sequence:

ATGAGCGAGG ACACGGTTCC CGAGGCGGCC TCCCCGCCAC CCTCTCAGGG GCAGCACTAC 
TTTGACCGGT TCTCTGAAGA TGATCCTGAG TACTTGCGAC TTCGCAACCG TGCAGCTGAC 
CTGCGACAGG ACTTCAACTT GATGGAGCAG AAGAAGAGGG TCACCATGAT CCTGCAGAGC 
CCTTCTTTCA GGGAGGAGCT GGAAGGCCTC ATCCAGGAGC AAATGAAGAA GGGCAACAAC 
TCCTCCAACA TCTGGGCCCT CCGACAGATC GCGGACTTCA TGGCCAGCAC CTCCCACGCA 
GTCTTCCCAG CTTCTTCCAT GAACTTCTCC ATGATGACAC CCATCAACGA CCTCCACACT 
GCCGACTCCC TGAACCTGGC CAAGGGGGAG AGGCTTATGC GGTGCAAGAT CAGCAGCGTC 
TACCGTCTCC TGGACCTCTA CGGCTGGGCG CAGCTCAGTG ACACCTACGT CACGCTGAGA 
GTGAGCAAGG AGCAGGACCA CTTCCTGATC AGCCCCAAGG GGGTTTCCTG CAGCGAGGTC 
ACAGCCTCCA GCCTGATTAA AGTGAACATT CTGGGAGAGG TGGTGGAGAA GGGCAGCAGT 
TGCTTCCCGG TGGACACCAC GGGCTTCAGT CTGCACTCAG CCATCTATGC CGCCAGGCCC 
GACGTGCGGT GTGCCATCCA CCTGCACACG CCTGCCACCG CAGCGGTGTC AGCTATGAAG 
TGCGGCCTCC TGCCGGTCTC CCATAATGCC CTGCTGGTGG GGGACATGGC CTACTATGAC 
TTCAATGGGG AAATGGAGCA GGAAGCTGAC CGAATCAACT TGCAGAAGTG CCTTGGACCC 
ACCTGCAAGA TTCTGGTGCT AAGAAACCAT GGCATGGTCG CCCTGGGTGA CACCGTGGAG 
GAAGCTTTCT ACAAGGTCTT CCATCTGCAG GCTGCGTGTG AGGTACAGGT GTCGGCTCTG 
TCCAGTGCTG GGGGCACTGA GAACCTCATC CTCTTGGAGC AAGAGAAACA CCGGCCGCAC 
GAGGTGGGCT CTGTGCAGTG GGCCGGCAGC ACCTTCGGGC CCATGCAGAA GAGCCGGCTG 
GGAGAGCATG AATTTGAAGC CCTCATGAGG ATGCTGGACA ATTTAGGCTA CAGAACAGGC 
TATACGTACC GCCACCCCTT TGTCCAAGAG AAAACCAAAC ACAAAAGTGA AGTGGAGATC 
CCAGCCACAG TCACTGCCTT TGTGTTTGAA GAGGATGGAG TCCCAGTCCC CGCCCTGCGC 
CAGCACGCCC AGAAGCAGCA GAAGGAAAAG ACCCGCTGGC TTAACACTCC CAACACCTAC 
CTGCGGGTGA ACGTGGCTGA CGAGGTGCAG AGGAACATGG GCAGTCCCCG ACCAAAGACC 
ACGTGGATGA AGGCTGATGA AGTGGAAAAG TCCAGCAGCG GCATGCCCAT ACGGATTGAA 
AACCCAAACC AATTTGTGCC TCTCTACACT GACCCCCAGG AAGTTCTGGA CATGAGGAAC 
AAGATTCGAG AGCAAAACCG ACAAGACATA AAGTCAGCCG GGCCTCAGTC TCAGCTCTTG 
GCCAGTGTCA TCGCAGAGAA GAGCCGGAGT CCGTCTACAG AGAGCCAGCT GATGTCCAAG 
GGCGATGCAG ATACCAAAGA TGAATCGGAG GAGACGGTGC CCAACCCCTT CAGCCAACTC 
ACTGACCAGG AGCTGGAGGA GTACAAGAAG GAGGTGGAAA GGAAGAAGCT AGAACAGGAG 
CAGGAAGGGG AGAAGGACAT AGCCACAGAG AAGCCTGGTT CACCTGTAAA GTCCACACCT 
GCATCCCCAG TGCAGAGCCC ATCAAAAGCC GGGACCAAGA GCCCAGCGGT CTCTCCTTCC 
AAGACTTCAG AGGATACCAA GAAGACAGAA GTCAGCGAAG CCAACACAGA GCCTGAGCCA 
GTGAAACCAG AAGGGCTGGT GGTGAATGGG AAGGAGGAGG AGCCGAGCGT AGAAGAGGCC 
CTCAGCAAAG GACTGGGCCA GATGACCACC AACGCTGACA CTGATGGCGA CAGCTACAAG 
GACAAAACCG AGTCAGTTAC CAGTGGCCCC TTGTCCCCAG AGGGCTCGCC CTCTAAGTCA 
CCCTCCAAAA AGAAAAAGAA ATTCCGAACC CCCTCGTTCC TGAAGAAAAG CAAGAAGAAG 
GAGAAGGTGG AATCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Add2 ( NM_001271859.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001258788.1 Mus musculus adducin 2 (beta) (Add2), transcript variant 4, mRNA.

Price & Availability↑

CloneID	OMu09405
Clone ID Related Accession (Same CDS sequence)	NM_001271858.1 , NM_001271859.1 , NM_013458.5 , NM_001271857.1
Accession Version	NM_001271859.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2178bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-23
Organism	Mus musculus(house mouse)
Product	beta-adducin isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC158645.6. Transcript Variant: This variant (4) differs in the 5' UTR compared to variant 1. Variants 1, 2, 3 and 4 encode the same isoform (1, also known as beta-1). Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK156954.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849388 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAGCGAGG ACACGGTTCC CGAGGCGGCC TCCCCGCCAC CCTCTCAGGG GCAGCACTAC 
TTTGACCGGT TCTCTGAAGA TGATCCTGAG TACTTGCGAC TTCGCAACCG TGCAGCTGAC 
CTGCGACAGG ACTTCAACTT GATGGAGCAG AAGAAGAGGG TCACCATGAT CCTGCAGAGC 
CCTTCTTTCA GGGAGGAGCT GGAAGGCCTC ATCCAGGAGC AAATGAAGAA GGGCAACAAC 
TCCTCCAACA TCTGGGCCCT CCGACAGATC GCGGACTTCA TGGCCAGCAC CTCCCACGCA 
GTCTTCCCAG CTTCTTCCAT GAACTTCTCC ATGATGACAC CCATCAACGA CCTCCACACT 
GCCGACTCCC TGAACCTGGC CAAGGGGGAG AGGCTTATGC GGTGCAAGAT CAGCAGCGTC 
TACCGTCTCC TGGACCTCTA CGGCTGGGCG CAGCTCAGTG ACACCTACGT CACGCTGAGA 
GTGAGCAAGG AGCAGGACCA CTTCCTGATC AGCCCCAAGG GGGTTTCCTG CAGCGAGGTC 
ACAGCCTCCA GCCTGATTAA AGTGAACATT CTGGGAGAGG TGGTGGAGAA GGGCAGCAGT 
TGCTTCCCGG TGGACACCAC GGGCTTCAGT CTGCACTCAG CCATCTATGC CGCCAGGCCC 
GACGTGCGGT GTGCCATCCA CCTGCACACG CCTGCCACCG CAGCGGTGTC AGCTATGAAG 
TGCGGCCTCC TGCCGGTCTC CCATAATGCC CTGCTGGTGG GGGACATGGC CTACTATGAC 
TTCAATGGGG AAATGGAGCA GGAAGCTGAC CGAATCAACT TGCAGAAGTG CCTTGGACCC 
ACCTGCAAGA TTCTGGTGCT AAGAAACCAT GGCATGGTCG CCCTGGGTGA CACCGTGGAG 
GAAGCTTTCT ACAAGGTCTT CCATCTGCAG GCTGCGTGTG AGGTACAGGT GTCGGCTCTG 
TCCAGTGCTG GGGGCACTGA GAACCTCATC CTCTTGGAGC AAGAGAAACA CCGGCCGCAC 
GAGGTGGGCT CTGTGCAGTG GGCCGGCAGC ACCTTCGGGC CCATGCAGAA GAGCCGGCTG 
GGAGAGCATG AATTTGAAGC CCTCATGAGG ATGCTGGACA ATTTAGGCTA CAGAACAGGC 
TATACGTACC GCCACCCCTT TGTCCAAGAG AAAACCAAAC ACAAAAGTGA AGTGGAGATC 
CCAGCCACAG TCACTGCCTT TGTGTTTGAA GAGGATGGAG TCCCAGTCCC CGCCCTGCGC 
CAGCACGCCC AGAAGCAGCA GAAGGAAAAG ACCCGCTGGC TTAACACTCC CAACACCTAC 
CTGCGGGTGA ACGTGGCTGA CGAGGTGCAG AGGAACATGG GCAGTCCCCG ACCAAAGACC 
ACGTGGATGA AGGCTGATGA AGTGGAAAAG TCCAGCAGCG GCATGCCCAT ACGGATTGAA 
AACCCAAACC AATTTGTGCC TCTCTACACT GACCCCCAGG AAGTTCTGGA CATGAGGAAC 
AAGATTCGAG AGCAAAACCG ACAAGACATA AAGTCAGCCG GGCCTCAGTC TCAGCTCTTG 
GCCAGTGTCA TCGCAGAGAA GAGCCGGAGT CCGTCTACAG AGAGCCAGCT GATGTCCAAG 
GGCGATGCAG ATACCAAAGA TGAATCGGAG GAGACGGTGC CCAACCCCTT CAGCCAACTC 
ACTGACCAGG AGCTGGAGGA GTACAAGAAG GAGGTGGAAA GGAAGAAGCT AGAACAGGAG 
CAGGAAGGGG AGAAGGACAT AGCCACAGAG AAGCCTGGTT CACCTGTAAA GTCCACACCT 
GCATCCCCAG TGCAGAGCCC ATCAAAAGCC GGGACCAAGA GCCCAGCGGT CTCTCCTTCC 
AAGACTTCAG AGGATACCAA GAAGACAGAA GTCAGCGAAG CCAACACAGA GCCTGAGCCA 
GTGAAACCAG AAGGGCTGGT GGTGAATGGG AAGGAGGAGG AGCCGAGCGT AGAAGAGGCC 
CTCAGCAAAG GACTGGGCCA GATGACCACC AACGCTGACA CTGATGGCGA CAGCTACAAG 
GACAAAACCG AGTCAGTTAC CAGTGGCCCC TTGTCCCCAG AGGGCTCGCC CTCTAAGTCA 
CCCTCCAAAA AGAAAAAGAA ATTCCGAACC CCCTCGTTCC TGAAGAAAAG CAAGAAGAAG 
GAGAAGGTGG AATCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001258788.1
CDS	201..2378
Translation	MSEDTVPEAASPPPSQGQHYFDRFSEDDPEYLRLRNRAADLRQDFNLMEQKKRVTMILQSPSFREELEGLIQEQMKKGNNSSNIWALRQIADFMASTSHAVFPASSMNFSMMTPINDLHTADSLNLAKGERLMRCKISSVYRLLDLYGWAQLSDTYVTLRVSKEQDHFLISPKGVSCSEVTASSLIKVNILGEVVEKGSSCFPVDTTGFSLHSAIYAARPDVRCAIHLHTPATAAVSAMKCGLLPVSHNALLVGDMAYYDFNGEMEQEADRINLQKCLGPTCKILVLRNHGMVALGDTVEEAFYKVFHLQAACEVQVSALSSAGGTENLILLEQEKHRPHEVGSVQWAGSTFGPMQKSRLGEHEFEALMRMLDNLGYRTGYTYRHPFVQEKTKHKSEVEIPATVTAFVFEEDGVPVPALRQHAQKQQKEKTRWLNTPNTYLRVNVADEVQRNMGSPRPKTTWMKADEVEKSSSGMPIRIENPNQFVPLYTDPQEVLDMRNKIREQNRQDIKSAGPQSQLLASVIAEKSRSPSTESQLMSKGDADTKDESEETVPNPFSQLTDQELEEYKKEVERKKLEQEQEGEKDIATEKPGSPVKSTPASPVQSPSKAGTKSPAVSPSKTSEDTKKTEVSEANTEPEPVKPEGLVVNGKEEEPSVEEALSKGLGQMTTNADTDGDSYKDKTESVTSGPLSPEGSPSKSPSKKKKKFRTPSFLKKSKKKEKVES

Target ORF information:

RefSeq Version	NM_001271859.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus adducin 2 (beta) (Add2), transcript variant 4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001271859.1

ORF Insert Sequence:

ATGAGCGAGG ACACGGTTCC CGAGGCGGCC TCCCCGCCAC CCTCTCAGGG GCAGCACTAC 
TTTGACCGGT TCTCTGAAGA TGATCCTGAG TACTTGCGAC TTCGCAACCG TGCAGCTGAC 
CTGCGACAGG ACTTCAACTT GATGGAGCAG AAGAAGAGGG TCACCATGAT CCTGCAGAGC 
CCTTCTTTCA GGGAGGAGCT GGAAGGCCTC ATCCAGGAGC AAATGAAGAA GGGCAACAAC 
TCCTCCAACA TCTGGGCCCT CCGACAGATC GCGGACTTCA TGGCCAGCAC CTCCCACGCA 
GTCTTCCCAG CTTCTTCCAT GAACTTCTCC ATGATGACAC CCATCAACGA CCTCCACACT 
GCCGACTCCC TGAACCTGGC CAAGGGGGAG AGGCTTATGC GGTGCAAGAT CAGCAGCGTC 
TACCGTCTCC TGGACCTCTA CGGCTGGGCG CAGCTCAGTG ACACCTACGT CACGCTGAGA 
GTGAGCAAGG AGCAGGACCA CTTCCTGATC AGCCCCAAGG GGGTTTCCTG CAGCGAGGTC 
ACAGCCTCCA GCCTGATTAA AGTGAACATT CTGGGAGAGG TGGTGGAGAA GGGCAGCAGT 
TGCTTCCCGG TGGACACCAC GGGCTTCAGT CTGCACTCAG CCATCTATGC CGCCAGGCCC 
GACGTGCGGT GTGCCATCCA CCTGCACACG CCTGCCACCG CAGCGGTGTC AGCTATGAAG 
TGCGGCCTCC TGCCGGTCTC CCATAATGCC CTGCTGGTGG GGGACATGGC CTACTATGAC 
TTCAATGGGG AAATGGAGCA GGAAGCTGAC CGAATCAACT TGCAGAAGTG CCTTGGACCC 
ACCTGCAAGA TTCTGGTGCT AAGAAACCAT GGCATGGTCG CCCTGGGTGA CACCGTGGAG 
GAAGCTTTCT ACAAGGTCTT CCATCTGCAG GCTGCGTGTG AGGTACAGGT GTCGGCTCTG 
TCCAGTGCTG GGGGCACTGA GAACCTCATC CTCTTGGAGC AAGAGAAACA CCGGCCGCAC 
GAGGTGGGCT CTGTGCAGTG GGCCGGCAGC ACCTTCGGGC CCATGCAGAA GAGCCGGCTG 
GGAGAGCATG AATTTGAAGC CCTCATGAGG ATGCTGGACA ATTTAGGCTA CAGAACAGGC 
TATACGTACC GCCACCCCTT TGTCCAAGAG AAAACCAAAC ACAAAAGTGA AGTGGAGATC 
CCAGCCACAG TCACTGCCTT TGTGTTTGAA GAGGATGGAG TCCCAGTCCC CGCCCTGCGC 
CAGCACGCCC AGAAGCAGCA GAAGGAAAAG ACCCGCTGGC TTAACACTCC CAACACCTAC 
CTGCGGGTGA ACGTGGCTGA CGAGGTGCAG AGGAACATGG GCAGTCCCCG ACCAAAGACC 
ACGTGGATGA AGGCTGATGA AGTGGAAAAG TCCAGCAGCG GCATGCCCAT ACGGATTGAA 
AACCCAAACC AATTTGTGCC TCTCTACACT GACCCCCAGG AAGTTCTGGA CATGAGGAAC 
AAGATTCGAG AGCAAAACCG ACAAGACATA AAGTCAGCCG GGCCTCAGTC TCAGCTCTTG 
GCCAGTGTCA TCGCAGAGAA GAGCCGGAGT CCGTCTACAG AGAGCCAGCT GATGTCCAAG 
GGCGATGCAG ATACCAAAGA TGAATCGGAG GAGACGGTGC CCAACCCCTT CAGCCAACTC 
ACTGACCAGG AGCTGGAGGA GTACAAGAAG GAGGTGGAAA GGAAGAAGCT AGAACAGGAG 
CAGGAAGGGG AGAAGGACAT AGCCACAGAG AAGCCTGGTT CACCTGTAAA GTCCACACCT 
GCATCCCCAG TGCAGAGCCC ATCAAAAGCC GGGACCAAGA GCCCAGCGGT CTCTCCTTCC 
AAGACTTCAG AGGATACCAA GAAGACAGAA GTCAGCGAAG CCAACACAGA GCCTGAGCCA 
GTGAAACCAG AAGGGCTGGT GGTGAATGGG AAGGAGGAGG AGCCGAGCGT AGAAGAGGCC 
CTCAGCAAAG GACTGGGCCA GATGACCACC AACGCTGACA CTGATGGCGA CAGCTACAAG 
GACAAAACCG AGTCAGTTAC CAGTGGCCCC TTGTCCCCAG AGGGCTCGCC CTCTAAGTCA 
CCCTCCAAAA AGAAAAAGAA ATTCCGAACC CCCTCGTTCC TGAAGAAAAG CAAGAAGAAG 
GAGAAGGTGG AATCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Add2 ( NM_013458.5 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_038486.2 Mus musculus adducin 2 (beta) (Add2), transcript variant 3, mRNA.

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CloneID	OMu09405
Clone ID Related Accession (Same CDS sequence)	NM_001271858.1 , NM_001271859.1 , NM_013458.5 , NM_001271857.1
Accession Version	NM_013458.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2178bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-27
Organism	Mus musculus(house mouse)
Product	beta-adducin isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC158645.6, AK014496.1, AK147544.1, CB722600.1, BU708888.1, CB524359.1, BU058008.1, BY712734.1, CB519116.1, BE951615.1 and AI835294.1. On Dec 5, 2012 this sequence version replaced NM_013458.4. Transcript Variant: This variant (3) differs in the 5' UTR compared to variant 1. Variants 1, 2, 3 and 4 encode the same isoform (1, also known as beta-1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK014496.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849388 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAGCGAGG ACACGGTTCC CGAGGCGGCC TCCCCGCCAC CCTCTCAGGG GCAGCACTAC 
TTTGACCGGT TCTCTGAAGA TGATCCTGAG TACTTGCGAC TTCGCAACCG TGCAGCTGAC 
CTGCGACAGG ACTTCAACTT GATGGAGCAG AAGAAGAGGG TCACCATGAT CCTGCAGAGC 
CCTTCTTTCA GGGAGGAGCT GGAAGGCCTC ATCCAGGAGC AAATGAAGAA GGGCAACAAC 
TCCTCCAACA TCTGGGCCCT CCGACAGATC GCGGACTTCA TGGCCAGCAC CTCCCACGCA 
GTCTTCCCAG CTTCTTCCAT GAACTTCTCC ATGATGACAC CCATCAACGA CCTCCACACT 
GCCGACTCCC TGAACCTGGC CAAGGGGGAG AGGCTTATGC GGTGCAAGAT CAGCAGCGTC 
TACCGTCTCC TGGACCTCTA CGGCTGGGCG CAGCTCAGTG ACACCTACGT CACGCTGAGA 
GTGAGCAAGG AGCAGGACCA CTTCCTGATC AGCCCCAAGG GGGTTTCCTG CAGCGAGGTC 
ACAGCCTCCA GCCTGATTAA AGTGAACATT CTGGGAGAGG TGGTGGAGAA GGGCAGCAGT 
TGCTTCCCGG TGGACACCAC GGGCTTCAGT CTGCACTCAG CCATCTATGC CGCCAGGCCC 
GACGTGCGGT GTGCCATCCA CCTGCACACG CCTGCCACCG CAGCGGTGTC AGCTATGAAG 
TGCGGCCTCC TGCCGGTCTC CCATAATGCC CTGCTGGTGG GGGACATGGC CTACTATGAC 
TTCAATGGGG AAATGGAGCA GGAAGCTGAC CGAATCAACT TGCAGAAGTG CCTTGGACCC 
ACCTGCAAGA TTCTGGTGCT AAGAAACCAT GGCATGGTCG CCCTGGGTGA CACCGTGGAG 
GAAGCTTTCT ACAAGGTCTT CCATCTGCAG GCTGCGTGTG AGGTACAGGT GTCGGCTCTG 
TCCAGTGCTG GGGGCACTGA GAACCTCATC CTCTTGGAGC AAGAGAAACA CCGGCCGCAC 
GAGGTGGGCT CTGTGCAGTG GGCCGGCAGC ACCTTCGGGC CCATGCAGAA GAGCCGGCTG 
GGAGAGCATG AATTTGAAGC CCTCATGAGG ATGCTGGACA ATTTAGGCTA CAGAACAGGC 
TATACGTACC GCCACCCCTT TGTCCAAGAG AAAACCAAAC ACAAAAGTGA AGTGGAGATC 
CCAGCCACAG TCACTGCCTT TGTGTTTGAA GAGGATGGAG TCCCAGTCCC CGCCCTGCGC 
CAGCACGCCC AGAAGCAGCA GAAGGAAAAG ACCCGCTGGC TTAACACTCC CAACACCTAC 
CTGCGGGTGA ACGTGGCTGA CGAGGTGCAG AGGAACATGG GCAGTCCCCG ACCAAAGACC 
ACGTGGATGA AGGCTGATGA AGTGGAAAAG TCCAGCAGCG GCATGCCCAT ACGGATTGAA 
AACCCAAACC AATTTGTGCC TCTCTACACT GACCCCCAGG AAGTTCTGGA CATGAGGAAC 
AAGATTCGAG AGCAAAACCG ACAAGACATA AAGTCAGCCG GGCCTCAGTC TCAGCTCTTG 
GCCAGTGTCA TCGCAGAGAA GAGCCGGAGT CCGTCTACAG AGAGCCAGCT GATGTCCAAG 
GGCGATGCAG ATACCAAAGA TGAATCGGAG GAGACGGTGC CCAACCCCTT CAGCCAACTC 
ACTGACCAGG AGCTGGAGGA GTACAAGAAG GAGGTGGAAA GGAAGAAGCT AGAACAGGAG 
CAGGAAGGGG AGAAGGACAT AGCCACAGAG AAGCCTGGTT CACCTGTAAA GTCCACACCT 
GCATCCCCAG TGCAGAGCCC ATCAAAAGCC GGGACCAAGA GCCCAGCGGT CTCTCCTTCC 
AAGACTTCAG AGGATACCAA GAAGACAGAA GTCAGCGAAG CCAACACAGA GCCTGAGCCA 
GTGAAACCAG AAGGGCTGGT GGTGAATGGG AAGGAGGAGG AGCCGAGCGT AGAAGAGGCC 
CTCAGCAAAG GACTGGGCCA GATGACCACC AACGCTGACA CTGATGGCGA CAGCTACAAG 
GACAAAACCG AGTCAGTTAC CAGTGGCCCC TTGTCCCCAG AGGGCTCGCC CTCTAAGTCA 
CCCTCCAAAA AGAAAAAGAA ATTCCGAACC CCCTCGTTCC TGAAGAAAAG CAAGAAGAAG 
GAGAAGGTGG AATCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_038486.2
CDS	204..2381
Translation	MSEDTVPEAASPPPSQGQHYFDRFSEDDPEYLRLRNRAADLRQDFNLMEQKKRVTMILQSPSFREELEGLIQEQMKKGNNSSNIWALRQIADFMASTSHAVFPASSMNFSMMTPINDLHTADSLNLAKGERLMRCKISSVYRLLDLYGWAQLSDTYVTLRVSKEQDHFLISPKGVSCSEVTASSLIKVNILGEVVEKGSSCFPVDTTGFSLHSAIYAARPDVRCAIHLHTPATAAVSAMKCGLLPVSHNALLVGDMAYYDFNGEMEQEADRINLQKCLGPTCKILVLRNHGMVALGDTVEEAFYKVFHLQAACEVQVSALSSAGGTENLILLEQEKHRPHEVGSVQWAGSTFGPMQKSRLGEHEFEALMRMLDNLGYRTGYTYRHPFVQEKTKHKSEVEIPATVTAFVFEEDGVPVPALRQHAQKQQKEKTRWLNTPNTYLRVNVADEVQRNMGSPRPKTTWMKADEVEKSSSGMPIRIENPNQFVPLYTDPQEVLDMRNKIREQNRQDIKSAGPQSQLLASVIAEKSRSPSTESQLMSKGDADTKDESEETVPNPFSQLTDQELEEYKKEVERKKLEQEQEGEKDIATEKPGSPVKSTPASPVQSPSKAGTKSPAVSPSKTSEDTKKTEVSEANTEPEPVKPEGLVVNGKEEEPSVEEALSKGLGQMTTNADTDGDSYKDKTESVTSGPLSPEGSPSKSPSKKKKKFRTPSFLKKSKKKEKVES

Target ORF information:

RefSeq Version	NM_013458.5
Organism	Mus musculus(house mouse)
Definition	Mus musculus adducin 2 (beta) (Add2), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_013458.5

ORF Insert Sequence:

ATGAGCGAGG ACACGGTTCC CGAGGCGGCC TCCCCGCCAC CCTCTCAGGG GCAGCACTAC 
TTTGACCGGT TCTCTGAAGA TGATCCTGAG TACTTGCGAC TTCGCAACCG TGCAGCTGAC 
CTGCGACAGG ACTTCAACTT GATGGAGCAG AAGAAGAGGG TCACCATGAT CCTGCAGAGC 
CCTTCTTTCA GGGAGGAGCT GGAAGGCCTC ATCCAGGAGC AAATGAAGAA GGGCAACAAC 
TCCTCCAACA TCTGGGCCCT CCGACAGATC GCGGACTTCA TGGCCAGCAC CTCCCACGCA 
GTCTTCCCAG CTTCTTCCAT GAACTTCTCC ATGATGACAC CCATCAACGA CCTCCACACT 
GCCGACTCCC TGAACCTGGC CAAGGGGGAG AGGCTTATGC GGTGCAAGAT CAGCAGCGTC 
TACCGTCTCC TGGACCTCTA CGGCTGGGCG CAGCTCAGTG ACACCTACGT CACGCTGAGA 
GTGAGCAAGG AGCAGGACCA CTTCCTGATC AGCCCCAAGG GGGTTTCCTG CAGCGAGGTC 
ACAGCCTCCA GCCTGATTAA AGTGAACATT CTGGGAGAGG TGGTGGAGAA GGGCAGCAGT 
TGCTTCCCGG TGGACACCAC GGGCTTCAGT CTGCACTCAG CCATCTATGC CGCCAGGCCC 
GACGTGCGGT GTGCCATCCA CCTGCACACG CCTGCCACCG CAGCGGTGTC AGCTATGAAG 
TGCGGCCTCC TGCCGGTCTC CCATAATGCC CTGCTGGTGG GGGACATGGC CTACTATGAC 
TTCAATGGGG AAATGGAGCA GGAAGCTGAC CGAATCAACT TGCAGAAGTG CCTTGGACCC 
ACCTGCAAGA TTCTGGTGCT AAGAAACCAT GGCATGGTCG CCCTGGGTGA CACCGTGGAG 
GAAGCTTTCT ACAAGGTCTT CCATCTGCAG GCTGCGTGTG AGGTACAGGT GTCGGCTCTG 
TCCAGTGCTG GGGGCACTGA GAACCTCATC CTCTTGGAGC AAGAGAAACA CCGGCCGCAC 
GAGGTGGGCT CTGTGCAGTG GGCCGGCAGC ACCTTCGGGC CCATGCAGAA GAGCCGGCTG 
GGAGAGCATG AATTTGAAGC CCTCATGAGG ATGCTGGACA ATTTAGGCTA CAGAACAGGC 
TATACGTACC GCCACCCCTT TGTCCAAGAG AAAACCAAAC ACAAAAGTGA AGTGGAGATC 
CCAGCCACAG TCACTGCCTT TGTGTTTGAA GAGGATGGAG TCCCAGTCCC CGCCCTGCGC 
CAGCACGCCC AGAAGCAGCA GAAGGAAAAG ACCCGCTGGC TTAACACTCC CAACACCTAC 
CTGCGGGTGA ACGTGGCTGA CGAGGTGCAG AGGAACATGG GCAGTCCCCG ACCAAAGACC 
ACGTGGATGA AGGCTGATGA AGTGGAAAAG TCCAGCAGCG GCATGCCCAT ACGGATTGAA 
AACCCAAACC AATTTGTGCC TCTCTACACT GACCCCCAGG AAGTTCTGGA CATGAGGAAC 
AAGATTCGAG AGCAAAACCG ACAAGACATA AAGTCAGCCG GGCCTCAGTC TCAGCTCTTG 
GCCAGTGTCA TCGCAGAGAA GAGCCGGAGT CCGTCTACAG AGAGCCAGCT GATGTCCAAG 
GGCGATGCAG ATACCAAAGA TGAATCGGAG GAGACGGTGC CCAACCCCTT CAGCCAACTC 
ACTGACCAGG AGCTGGAGGA GTACAAGAAG GAGGTGGAAA GGAAGAAGCT AGAACAGGAG 
CAGGAAGGGG AGAAGGACAT AGCCACAGAG AAGCCTGGTT CACCTGTAAA GTCCACACCT 
GCATCCCCAG TGCAGAGCCC ATCAAAAGCC GGGACCAAGA GCCCAGCGGT CTCTCCTTCC 
AAGACTTCAG AGGATACCAA GAAGACAGAA GTCAGCGAAG CCAACACAGA GCCTGAGCCA 
GTGAAACCAG AAGGGCTGGT GGTGAATGGG AAGGAGGAGG AGCCGAGCGT AGAAGAGGCC 
CTCAGCAAAG GACTGGGCCA GATGACCACC AACGCTGACA CTGATGGCGA CAGCTACAAG 
GACAAAACCG AGTCAGTTAC CAGTGGCCCC TTGTCCCCAG AGGGCTCGCC CTCTAAGTCA 
CCCTCCAAAA AGAAAAAGAA ATTCCGAACC CCCTCGTTCC TGAAGAAAAG CAAGAAGAAG 
GAGAAGGTGG AATCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Add2 ( NM_001271857.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001258786.1 Mus musculus adducin 2 (beta) (Add2), transcript variant 1, mRNA.

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CloneID	OMu09405
Clone ID Related Accession (Same CDS sequence)	NM_001271858.1 , NM_001271859.1 , NM_013458.5 , NM_001271857.1
Accession Version	NM_001271857.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2178bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-23
Organism	Mus musculus(house mouse)
Product	beta-adducin isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BY146240.1, AK147544.1, AK014496.1, CB722600.1, BU708888.1, CB524359.1, BU058008.1, BY712734.1, CB519116.1, BE951615.1 and AI835294.1. Transcript Variant: This variant (1) represents the longest transcript and encodes the longer isoform (1). Variants 1, 2, 3 and 4 encode the same isoform (1, also known as beta-1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK147544.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN01164131 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAGCGAGG ACACGGTTCC CGAGGCGGCC TCCCCGCCAC CCTCTCAGGG GCAGCACTAC 
TTTGACCGGT TCTCTGAAGA TGATCCTGAG TACTTGCGAC TTCGCAACCG TGCAGCTGAC 
CTGCGACAGG ACTTCAACTT GATGGAGCAG AAGAAGAGGG TCACCATGAT CCTGCAGAGC 
CCTTCTTTCA GGGAGGAGCT GGAAGGCCTC ATCCAGGAGC AAATGAAGAA GGGCAACAAC 
TCCTCCAACA TCTGGGCCCT CCGACAGATC GCGGACTTCA TGGCCAGCAC CTCCCACGCA 
GTCTTCCCAG CTTCTTCCAT GAACTTCTCC ATGATGACAC CCATCAACGA CCTCCACACT 
GCCGACTCCC TGAACCTGGC CAAGGGGGAG AGGCTTATGC GGTGCAAGAT CAGCAGCGTC 
TACCGTCTCC TGGACCTCTA CGGCTGGGCG CAGCTCAGTG ACACCTACGT CACGCTGAGA 
GTGAGCAAGG AGCAGGACCA CTTCCTGATC AGCCCCAAGG GGGTTTCCTG CAGCGAGGTC 
ACAGCCTCCA GCCTGATTAA AGTGAACATT CTGGGAGAGG TGGTGGAGAA GGGCAGCAGT 
TGCTTCCCGG TGGACACCAC GGGCTTCAGT CTGCACTCAG CCATCTATGC CGCCAGGCCC 
GACGTGCGGT GTGCCATCCA CCTGCACACG CCTGCCACCG CAGCGGTGTC AGCTATGAAG 
TGCGGCCTCC TGCCGGTCTC CCATAATGCC CTGCTGGTGG GGGACATGGC CTACTATGAC 
TTCAATGGGG AAATGGAGCA GGAAGCTGAC CGAATCAACT TGCAGAAGTG CCTTGGACCC 
ACCTGCAAGA TTCTGGTGCT AAGAAACCAT GGCATGGTCG CCCTGGGTGA CACCGTGGAG 
GAAGCTTTCT ACAAGGTCTT CCATCTGCAG GCTGCGTGTG AGGTACAGGT GTCGGCTCTG 
TCCAGTGCTG GGGGCACTGA GAACCTCATC CTCTTGGAGC AAGAGAAACA CCGGCCGCAC 
GAGGTGGGCT CTGTGCAGTG GGCCGGCAGC ACCTTCGGGC CCATGCAGAA GAGCCGGCTG 
GGAGAGCATG AATTTGAAGC CCTCATGAGG ATGCTGGACA ATTTAGGCTA CAGAACAGGC 
TATACGTACC GCCACCCCTT TGTCCAAGAG AAAACCAAAC ACAAAAGTGA AGTGGAGATC 
CCAGCCACAG TCACTGCCTT TGTGTTTGAA GAGGATGGAG TCCCAGTCCC CGCCCTGCGC 
CAGCACGCCC AGAAGCAGCA GAAGGAAAAG ACCCGCTGGC TTAACACTCC CAACACCTAC 
CTGCGGGTGA ACGTGGCTGA CGAGGTGCAG AGGAACATGG GCAGTCCCCG ACCAAAGACC 
ACGTGGATGA AGGCTGATGA AGTGGAAAAG TCCAGCAGCG GCATGCCCAT ACGGATTGAA 
AACCCAAACC AATTTGTGCC TCTCTACACT GACCCCCAGG AAGTTCTGGA CATGAGGAAC 
AAGATTCGAG AGCAAAACCG ACAAGACATA AAGTCAGCCG GGCCTCAGTC TCAGCTCTTG 
GCCAGTGTCA TCGCAGAGAA GAGCCGGAGT CCGTCTACAG AGAGCCAGCT GATGTCCAAG 
GGCGATGCAG ATACCAAAGA TGAATCGGAG GAGACGGTGC CCAACCCCTT CAGCCAACTC 
ACTGACCAGG AGCTGGAGGA GTACAAGAAG GAGGTGGAAA GGAAGAAGCT AGAACAGGAG 
CAGGAAGGGG AGAAGGACAT AGCCACAGAG AAGCCTGGTT CACCTGTAAA GTCCACACCT 
GCATCCCCAG TGCAGAGCCC ATCAAAAGCC GGGACCAAGA GCCCAGCGGT CTCTCCTTCC 
AAGACTTCAG AGGATACCAA GAAGACAGAA GTCAGCGAAG CCAACACAGA GCCTGAGCCA 
GTGAAACCAG AAGGGCTGGT GGTGAATGGG AAGGAGGAGG AGCCGAGCGT AGAAGAGGCC 
CTCAGCAAAG GACTGGGCCA GATGACCACC AACGCTGACA CTGATGGCGA CAGCTACAAG 
GACAAAACCG AGTCAGTTAC CAGTGGCCCC TTGTCCCCAG AGGGCTCGCC CTCTAAGTCA 
CCCTCCAAAA AGAAAAAGAA ATTCCGAACC CCCTCGTTCC TGAAGAAAAG CAAGAAGAAG 
GAGAAGGTGG AATCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001258786.1
CDS	403..2580
Translation	MSEDTVPEAASPPPSQGQHYFDRFSEDDPEYLRLRNRAADLRQDFNLMEQKKRVTMILQSPSFREELEGLIQEQMKKGNNSSNIWALRQIADFMASTSHAVFPASSMNFSMMTPINDLHTADSLNLAKGERLMRCKISSVYRLLDLYGWAQLSDTYVTLRVSKEQDHFLISPKGVSCSEVTASSLIKVNILGEVVEKGSSCFPVDTTGFSLHSAIYAARPDVRCAIHLHTPATAAVSAMKCGLLPVSHNALLVGDMAYYDFNGEMEQEADRINLQKCLGPTCKILVLRNHGMVALGDTVEEAFYKVFHLQAACEVQVSALSSAGGTENLILLEQEKHRPHEVGSVQWAGSTFGPMQKSRLGEHEFEALMRMLDNLGYRTGYTYRHPFVQEKTKHKSEVEIPATVTAFVFEEDGVPVPALRQHAQKQQKEKTRWLNTPNTYLRVNVADEVQRNMGSPRPKTTWMKADEVEKSSSGMPIRIENPNQFVPLYTDPQEVLDMRNKIREQNRQDIKSAGPQSQLLASVIAEKSRSPSTESQLMSKGDADTKDESEETVPNPFSQLTDQELEEYKKEVERKKLEQEQEGEKDIATEKPGSPVKSTPASPVQSPSKAGTKSPAVSPSKTSEDTKKTEVSEANTEPEPVKPEGLVVNGKEEEPSVEEALSKGLGQMTTNADTDGDSYKDKTESVTSGPLSPEGSPSKSPSKKKKKFRTPSFLKKSKKKEKVES

Target ORF information:

RefSeq Version	NM_001271857.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus adducin 2 (beta) (Add2), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001271857.1

ORF Insert Sequence:

ATGAGCGAGG ACACGGTTCC CGAGGCGGCC TCCCCGCCAC CCTCTCAGGG GCAGCACTAC 
TTTGACCGGT TCTCTGAAGA TGATCCTGAG TACTTGCGAC TTCGCAACCG TGCAGCTGAC 
CTGCGACAGG ACTTCAACTT GATGGAGCAG AAGAAGAGGG TCACCATGAT CCTGCAGAGC 
CCTTCTTTCA GGGAGGAGCT GGAAGGCCTC ATCCAGGAGC AAATGAAGAA GGGCAACAAC 
TCCTCCAACA TCTGGGCCCT CCGACAGATC GCGGACTTCA TGGCCAGCAC CTCCCACGCA 
GTCTTCCCAG CTTCTTCCAT GAACTTCTCC ATGATGACAC CCATCAACGA CCTCCACACT 
GCCGACTCCC TGAACCTGGC CAAGGGGGAG AGGCTTATGC GGTGCAAGAT CAGCAGCGTC 
TACCGTCTCC TGGACCTCTA CGGCTGGGCG CAGCTCAGTG ACACCTACGT CACGCTGAGA 
GTGAGCAAGG AGCAGGACCA CTTCCTGATC AGCCCCAAGG GGGTTTCCTG CAGCGAGGTC 
ACAGCCTCCA GCCTGATTAA AGTGAACATT CTGGGAGAGG TGGTGGAGAA GGGCAGCAGT 
TGCTTCCCGG TGGACACCAC GGGCTTCAGT CTGCACTCAG CCATCTATGC CGCCAGGCCC 
GACGTGCGGT GTGCCATCCA CCTGCACACG CCTGCCACCG CAGCGGTGTC AGCTATGAAG 
TGCGGCCTCC TGCCGGTCTC CCATAATGCC CTGCTGGTGG GGGACATGGC CTACTATGAC 
TTCAATGGGG AAATGGAGCA GGAAGCTGAC CGAATCAACT TGCAGAAGTG CCTTGGACCC 
ACCTGCAAGA TTCTGGTGCT AAGAAACCAT GGCATGGTCG CCCTGGGTGA CACCGTGGAG 
GAAGCTTTCT ACAAGGTCTT CCATCTGCAG GCTGCGTGTG AGGTACAGGT GTCGGCTCTG 
TCCAGTGCTG GGGGCACTGA GAACCTCATC CTCTTGGAGC AAGAGAAACA CCGGCCGCAC 
GAGGTGGGCT CTGTGCAGTG GGCCGGCAGC ACCTTCGGGC CCATGCAGAA GAGCCGGCTG 
GGAGAGCATG AATTTGAAGC CCTCATGAGG ATGCTGGACA ATTTAGGCTA CAGAACAGGC 
TATACGTACC GCCACCCCTT TGTCCAAGAG AAAACCAAAC ACAAAAGTGA AGTGGAGATC 
CCAGCCACAG TCACTGCCTT TGTGTTTGAA GAGGATGGAG TCCCAGTCCC CGCCCTGCGC 
CAGCACGCCC AGAAGCAGCA GAAGGAAAAG ACCCGCTGGC TTAACACTCC CAACACCTAC 
CTGCGGGTGA ACGTGGCTGA CGAGGTGCAG AGGAACATGG GCAGTCCCCG ACCAAAGACC 
ACGTGGATGA AGGCTGATGA AGTGGAAAAG TCCAGCAGCG GCATGCCCAT ACGGATTGAA 
AACCCAAACC AATTTGTGCC TCTCTACACT GACCCCCAGG AAGTTCTGGA CATGAGGAAC 
AAGATTCGAG AGCAAAACCG ACAAGACATA AAGTCAGCCG GGCCTCAGTC TCAGCTCTTG 
GCCAGTGTCA TCGCAGAGAA GAGCCGGAGT CCGTCTACAG AGAGCCAGCT GATGTCCAAG 
GGCGATGCAG ATACCAAAGA TGAATCGGAG GAGACGGTGC CCAACCCCTT CAGCCAACTC 
ACTGACCAGG AGCTGGAGGA GTACAAGAAG GAGGTGGAAA GGAAGAAGCT AGAACAGGAG 
CAGGAAGGGG AGAAGGACAT AGCCACAGAG AAGCCTGGTT CACCTGTAAA GTCCACACCT 
GCATCCCCAG TGCAGAGCCC ATCAAAAGCC GGGACCAAGA GCCCAGCGGT CTCTCCTTCC 
AAGACTTCAG AGGATACCAA GAAGACAGAA GTCAGCGAAG CCAACACAGA GCCTGAGCCA 
GTGAAACCAG AAGGGCTGGT GGTGAATGGG AAGGAGGAGG AGCCGAGCGT AGAAGAGGCC 
CTCAGCAAAG GACTGGGCCA GATGACCACC AACGCTGACA CTGATGGCGA CAGCTACAAG 
GACAAAACCG AGTCAGTTAC CAGTGGCCCC TTGTCCCCAG AGGGCTCGCC CTCTAAGTCA 
CCCTCCAAAA AGAAAAAGAA ATTCCGAACC CCCTCGTTCC TGAAGAAAAG CAAGAAGAAG 
GAGAAGGTGG AATCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Add2 ( NM_001271861.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001258790.1 Mus musculus adducin 2 (beta) (Add2), transcript variant 6, mRNA.

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CloneID	OMu07382
Clone ID Related Accession (Same CDS sequence)	NM_001271861.1 , NM_001271860.1
Accession Version	NM_001271861.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1689bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-24
Organism	Mus musculus(house mouse)
Product	beta-adducin isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC158645.6, AK028425.1 and BC053032.1. Transcript Variant: This variant (6) differs in the 5' UTR, lacks several 3' exons and contains an alternate 3' exon, and thus differs in the 3' coding region and 3' UTR, compared to variant 1. The encoded isoform (2) has a distinct C-terminus and is shorter than isoform 1. Both variants 5 and 6 encode the same isoform (2, also known as beta-2). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK028425.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849388 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAGCGAGG ACACGGTTCC CGAGGCGGCC TCCCCGCCAC CCTCTCAGGG GCAGCACTAC 
TTTGACCGGT TCTCTGAAGA TGATCCTGAG TACTTGCGAC TTCGCAACCG TGCAGCTGAC 
CTGCGACAGG ACTTCAACTT GATGGAGCAG AAGAAGAGGG TCACCATGAT CCTGCAGAGC 
CCTTCTTTCA GGGAGGAGCT GGAAGGCCTC ATCCAGGAGC AAATGAAGAA GGGCAACAAC 
TCCTCCAACA TCTGGGCCCT CCGACAGATC GCGGACTTCA TGGCCAGCAC CTCCCACGCA 
GTCTTCCCAG CTTCTTCCAT GAACTTCTCC ATGATGACAC CCATCAACGA CCTCCACACT 
GCCGACTCCC TGAACCTGGC CAAGGGGGAG AGGCTTATGC GGTGCAAGAT CAGCAGCGTC 
TACCGTCTCC TGGACCTCTA CGGCTGGGCG CAGCTCAGTG ACACCTACGT CACGCTGAGA 
GTGAGCAAGG AGCAGGACCA CTTCCTGATC AGCCCCAAGG GGGTTTCCTG CAGCGAGGTC 
ACAGCCTCCA GCCTGATTAA AGTGAACATT CTGGGAGAGG TGGTGGAGAA GGGCAGCAGT 
TGCTTCCCGG TGGACACCAC GGGCTTCAGT CTGCACTCAG CCATCTATGC CGCCAGGCCC 
GACGTGCGGT GTGCCATCCA CCTGCACACG CCTGCCACCG CAGCGGTGTC AGCTATGAAG 
TGCGGCCTCC TGCCGGTCTC CCATAATGCC CTGCTGGTGG GGGACATGGC CTACTATGAC 
TTCAATGGGG AAATGGAGCA GGAAGCTGAC CGAATCAACT TGCAGAAGTG CCTTGGACCC 
ACCTGCAAGA TTCTGGTGCT AAGAAACCAT GGCATGGTCG CCCTGGGTGA CACCGTGGAG 
GAAGCTTTCT ACAAGGTCTT CCATCTGCAG GCTGCGTGTG AGGTACAGGT GTCGGCTCTG 
TCCAGTGCTG GGGGCACTGA GAACCTCATC CTCTTGGAGC AAGAGAAACA CCGGCCGCAC 
GAGGTGGGCT CTGTGCAGTG GGCCGGCAGC ACCTTCGGGC CCATGCAGAA GAGCCGGCTG 
GGAGAGCATG AATTTGAAGC CCTCATGAGG ATGCTGGACA ATTTAGGCTA CAGAACAGGC 
TATACGTACC GCCACCCCTT TGTCCAAGAG AAAACCAAAC ACAAAAGTGA AGTGGAGATC 
CCAGCCACAG TCACTGCCTT TGTGTTTGAA GAGGATGGAG TCCCAGTCCC CGCCCTGCGC 
CAGCACGCCC AGAAGCAGCA GAAGGAAAAG ACCCGCTGGC TTAACACTCC CAACACCTAC 
CTGCGGGTGA ACGTGGCTGA CGAGGTGCAG AGGAACATGG GCAGTCCCCG ACCAAAGACC 
ACGTGGATGA AGGCTGATGA AGTGGAAAAG TCCAGCAGCG GCATGCCCAT ACGGATTGAA 
AACCCAAACC AATTTGTGCC TCTCTACACT GACCCCCAGG AAGTTCTGGA CATGAGGAAC 
AAGATTCGAG AGCAAAACCG ACAAGACATA AAGTCAGCCG GGCCTCAGTC TCAGCTCTTG 
GCCAGTGTCA TCGCAGAGAA GAGCCGGAGT CCGGTACAGC AGAGACTGCC CCCAACCGAA 
GGGGAAGTGT ATCAGACTCC TGGGGCTGGG CAGGGGACCC CTGAGTCCTC AGGCCCGCTC 
ACCCCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001258790.1
CDS	204..1892
Translation	MSEDTVPEAASPPPSQGQHYFDRFSEDDPEYLRLRNRAADLRQDFNLMEQKKRVTMILQSPSFREELEGLIQEQMKKGNNSSNIWALRQIADFMASTSHAVFPASSMNFSMMTPINDLHTADSLNLAKGERLMRCKISSVYRLLDLYGWAQLSDTYVTLRVSKEQDHFLISPKGVSCSEVTASSLIKVNILGEVVEKGSSCFPVDTTGFSLHSAIYAARPDVRCAIHLHTPATAAVSAMKCGLLPVSHNALLVGDMAYYDFNGEMEQEADRINLQKCLGPTCKILVLRNHGMVALGDTVEEAFYKVFHLQAACEVQVSALSSAGGTENLILLEQEKHRPHEVGSVQWAGSTFGPMQKSRLGEHEFEALMRMLDNLGYRTGYTYRHPFVQEKTKHKSEVEIPATVTAFVFEEDGVPVPALRQHAQKQQKEKTRWLNTPNTYLRVNVADEVQRNMGSPRPKTTWMKADEVEKSSSGMPIRIENPNQFVPLYTDPQEVLDMRNKIREQNRQDIKSAGPQSQLLASVIAEKSRSPVQQRLPPTEGEVYQTPGAGQGTPESSGPLTP

Target ORF information:

RefSeq Version	NM_001271861.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus adducin 2 (beta) (Add2), transcript variant 6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001271861.1

ORF Insert Sequence:

ATGAGCGAGG ACACGGTTCC CGAGGCGGCC TCCCCGCCAC CCTCTCAGGG GCAGCACTAC 
TTTGACCGGT TCTCTGAAGA TGATCCTGAG TACTTGCGAC TTCGCAACCG TGCAGCTGAC 
CTGCGACAGG ACTTCAACTT GATGGAGCAG AAGAAGAGGG TCACCATGAT CCTGCAGAGC 
CCTTCTTTCA GGGAGGAGCT GGAAGGCCTC ATCCAGGAGC AAATGAAGAA GGGCAACAAC 
TCCTCCAACA TCTGGGCCCT CCGACAGATC GCGGACTTCA TGGCCAGCAC CTCCCACGCA 
GTCTTCCCAG CTTCTTCCAT GAACTTCTCC ATGATGACAC CCATCAACGA CCTCCACACT 
GCCGACTCCC TGAACCTGGC CAAGGGGGAG AGGCTTATGC GGTGCAAGAT CAGCAGCGTC 
TACCGTCTCC TGGACCTCTA CGGCTGGGCG CAGCTCAGTG ACACCTACGT CACGCTGAGA 
GTGAGCAAGG AGCAGGACCA CTTCCTGATC AGCCCCAAGG GGGTTTCCTG CAGCGAGGTC 
ACAGCCTCCA GCCTGATTAA AGTGAACATT CTGGGAGAGG TGGTGGAGAA GGGCAGCAGT 
TGCTTCCCGG TGGACACCAC GGGCTTCAGT CTGCACTCAG CCATCTATGC CGCCAGGCCC 
GACGTGCGGT GTGCCATCCA CCTGCACACG CCTGCCACCG CAGCGGTGTC AGCTATGAAG 
TGCGGCCTCC TGCCGGTCTC CCATAATGCC CTGCTGGTGG GGGACATGGC CTACTATGAC 
TTCAATGGGG AAATGGAGCA GGAAGCTGAC CGAATCAACT TGCAGAAGTG CCTTGGACCC 
ACCTGCAAGA TTCTGGTGCT AAGAAACCAT GGCATGGTCG CCCTGGGTGA CACCGTGGAG 
GAAGCTTTCT ACAAGGTCTT CCATCTGCAG GCTGCGTGTG AGGTACAGGT GTCGGCTCTG 
TCCAGTGCTG GGGGCACTGA GAACCTCATC CTCTTGGAGC AAGAGAAACA CCGGCCGCAC 
GAGGTGGGCT CTGTGCAGTG GGCCGGCAGC ACCTTCGGGC CCATGCAGAA GAGCCGGCTG 
GGAGAGCATG AATTTGAAGC CCTCATGAGG ATGCTGGACA ATTTAGGCTA CAGAACAGGC 
TATACGTACC GCCACCCCTT TGTCCAAGAG AAAACCAAAC ACAAAAGTGA AGTGGAGATC 
CCAGCCACAG TCACTGCCTT TGTGTTTGAA GAGGATGGAG TCCCAGTCCC CGCCCTGCGC 
CAGCACGCCC AGAAGCAGCA GAAGGAAAAG ACCCGCTGGC TTAACACTCC CAACACCTAC 
CTGCGGGTGA ACGTGGCTGA CGAGGTGCAG AGGAACATGG GCAGTCCCCG ACCAAAGACC 
ACGTGGATGA AGGCTGATGA AGTGGAAAAG TCCAGCAGCG GCATGCCCAT ACGGATTGAA 
AACCCAAACC AATTTGTGCC TCTCTACACT GACCCCCAGG AAGTTCTGGA CATGAGGAAC 
AAGATTCGAG AGCAAAACCG ACAAGACATA AAGTCAGCCG GGCCTCAGTC TCAGCTCTTG 
GCCAGTGTCA TCGCAGAGAA GAGCCGGAGT CCGGTACAGC AGAGACTGCC CCCAACCGAA 
GGGGAAGTGT ATCAGACTCC TGGGGCTGGG CAGGGGACCC CTGAGTCCTC AGGCCCGCTC 
ACCCCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Add2 ( NM_001271860.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001258789.1 Mus musculus adducin 2 (beta) (Add2), transcript variant 5, mRNA.

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CloneID	OMu07382
Clone ID Related Accession (Same CDS sequence)	NM_001271861.1 , NM_001271860.1
Accession Version	NM_001271860.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1689bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-27
Organism	Mus musculus(house mouse)
Product	beta-adducin isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BY146240.1 and BC053032.1. Transcript Variant: This variant (5) lacks several 3' exons and contains an alternate 3' exon, and thus differs in the 3' coding region and 3' UTR, compared to variant 1. The encoded isoform (2) has a distinct C-terminus and is shorter than isoform 1. Both variants 5 and 6 encode the same isoform (2, also known as beta-2). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC046783.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849385 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAGCGAGG ACACGGTTCC CGAGGCGGCC TCCCCGCCAC CCTCTCAGGG GCAGCACTAC 
TTTGACCGGT TCTCTGAAGA TGATCCTGAG TACTTGCGAC TTCGCAACCG TGCAGCTGAC 
CTGCGACAGG ACTTCAACTT GATGGAGCAG AAGAAGAGGG TCACCATGAT CCTGCAGAGC 
CCTTCTTTCA GGGAGGAGCT GGAAGGCCTC ATCCAGGAGC AAATGAAGAA GGGCAACAAC 
TCCTCCAACA TCTGGGCCCT CCGACAGATC GCGGACTTCA TGGCCAGCAC CTCCCACGCA 
GTCTTCCCAG CTTCTTCCAT GAACTTCTCC ATGATGACAC CCATCAACGA CCTCCACACT 
GCCGACTCCC TGAACCTGGC CAAGGGGGAG AGGCTTATGC GGTGCAAGAT CAGCAGCGTC 
TACCGTCTCC TGGACCTCTA CGGCTGGGCG CAGCTCAGTG ACACCTACGT CACGCTGAGA 
GTGAGCAAGG AGCAGGACCA CTTCCTGATC AGCCCCAAGG GGGTTTCCTG CAGCGAGGTC 
ACAGCCTCCA GCCTGATTAA AGTGAACATT CTGGGAGAGG TGGTGGAGAA GGGCAGCAGT 
TGCTTCCCGG TGGACACCAC GGGCTTCAGT CTGCACTCAG CCATCTATGC CGCCAGGCCC 
GACGTGCGGT GTGCCATCCA CCTGCACACG CCTGCCACCG CAGCGGTGTC AGCTATGAAG 
TGCGGCCTCC TGCCGGTCTC CCATAATGCC CTGCTGGTGG GGGACATGGC CTACTATGAC 
TTCAATGGGG AAATGGAGCA GGAAGCTGAC CGAATCAACT TGCAGAAGTG CCTTGGACCC 
ACCTGCAAGA TTCTGGTGCT AAGAAACCAT GGCATGGTCG CCCTGGGTGA CACCGTGGAG 
GAAGCTTTCT ACAAGGTCTT CCATCTGCAG GCTGCGTGTG AGGTACAGGT GTCGGCTCTG 
TCCAGTGCTG GGGGCACTGA GAACCTCATC CTCTTGGAGC AAGAGAAACA CCGGCCGCAC 
GAGGTGGGCT CTGTGCAGTG GGCCGGCAGC ACCTTCGGGC CCATGCAGAA GAGCCGGCTG 
GGAGAGCATG AATTTGAAGC CCTCATGAGG ATGCTGGACA ATTTAGGCTA CAGAACAGGC 
TATACGTACC GCCACCCCTT TGTCCAAGAG AAAACCAAAC ACAAAAGTGA AGTGGAGATC 
CCAGCCACAG TCACTGCCTT TGTGTTTGAA GAGGATGGAG TCCCAGTCCC CGCCCTGCGC 
CAGCACGCCC AGAAGCAGCA GAAGGAAAAG ACCCGCTGGC TTAACACTCC CAACACCTAC 
CTGCGGGTGA ACGTGGCTGA CGAGGTGCAG AGGAACATGG GCAGTCCCCG ACCAAAGACC 
ACGTGGATGA AGGCTGATGA AGTGGAAAAG TCCAGCAGCG GCATGCCCAT ACGGATTGAA 
AACCCAAACC AATTTGTGCC TCTCTACACT GACCCCCAGG AAGTTCTGGA CATGAGGAAC 
AAGATTCGAG AGCAAAACCG ACAAGACATA AAGTCAGCCG GGCCTCAGTC TCAGCTCTTG 
GCCAGTGTCA TCGCAGAGAA GAGCCGGAGT CCGGTACAGC AGAGACTGCC CCCAACCGAA 
GGGGAAGTGT ATCAGACTCC TGGGGCTGGG CAGGGGACCC CTGAGTCCTC AGGCCCGCTC 
ACCCCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001258789.1
CDS	403..2091
Translation	MSEDTVPEAASPPPSQGQHYFDRFSEDDPEYLRLRNRAADLRQDFNLMEQKKRVTMILQSPSFREELEGLIQEQMKKGNNSSNIWALRQIADFMASTSHAVFPASSMNFSMMTPINDLHTADSLNLAKGERLMRCKISSVYRLLDLYGWAQLSDTYVTLRVSKEQDHFLISPKGVSCSEVTASSLIKVNILGEVVEKGSSCFPVDTTGFSLHSAIYAARPDVRCAIHLHTPATAAVSAMKCGLLPVSHNALLVGDMAYYDFNGEMEQEADRINLQKCLGPTCKILVLRNHGMVALGDTVEEAFYKVFHLQAACEVQVSALSSAGGTENLILLEQEKHRPHEVGSVQWAGSTFGPMQKSRLGEHEFEALMRMLDNLGYRTGYTYRHPFVQEKTKHKSEVEIPATVTAFVFEEDGVPVPALRQHAQKQQKEKTRWLNTPNTYLRVNVADEVQRNMGSPRPKTTWMKADEVEKSSSGMPIRIENPNQFVPLYTDPQEVLDMRNKIREQNRQDIKSAGPQSQLLASVIAEKSRSPVQQRLPPTEGEVYQTPGAGQGTPESSGPLTP

Target ORF information:

RefSeq Version	NM_001271860.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus adducin 2 (beta) (Add2), transcript variant 5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001271860.1

ORF Insert Sequence:

ATGAGCGAGG ACACGGTTCC CGAGGCGGCC TCCCCGCCAC CCTCTCAGGG GCAGCACTAC 
TTTGACCGGT TCTCTGAAGA TGATCCTGAG TACTTGCGAC TTCGCAACCG TGCAGCTGAC 
CTGCGACAGG ACTTCAACTT GATGGAGCAG AAGAAGAGGG TCACCATGAT CCTGCAGAGC 
CCTTCTTTCA GGGAGGAGCT GGAAGGCCTC ATCCAGGAGC AAATGAAGAA GGGCAACAAC 
TCCTCCAACA TCTGGGCCCT CCGACAGATC GCGGACTTCA TGGCCAGCAC CTCCCACGCA 
GTCTTCCCAG CTTCTTCCAT GAACTTCTCC ATGATGACAC CCATCAACGA CCTCCACACT 
GCCGACTCCC TGAACCTGGC CAAGGGGGAG AGGCTTATGC GGTGCAAGAT CAGCAGCGTC 
TACCGTCTCC TGGACCTCTA CGGCTGGGCG CAGCTCAGTG ACACCTACGT CACGCTGAGA 
GTGAGCAAGG AGCAGGACCA CTTCCTGATC AGCCCCAAGG GGGTTTCCTG CAGCGAGGTC 
ACAGCCTCCA GCCTGATTAA AGTGAACATT CTGGGAGAGG TGGTGGAGAA GGGCAGCAGT 
TGCTTCCCGG TGGACACCAC GGGCTTCAGT CTGCACTCAG CCATCTATGC CGCCAGGCCC 
GACGTGCGGT GTGCCATCCA CCTGCACACG CCTGCCACCG CAGCGGTGTC AGCTATGAAG 
TGCGGCCTCC TGCCGGTCTC CCATAATGCC CTGCTGGTGG GGGACATGGC CTACTATGAC 
TTCAATGGGG AAATGGAGCA GGAAGCTGAC CGAATCAACT TGCAGAAGTG CCTTGGACCC 
ACCTGCAAGA TTCTGGTGCT AAGAAACCAT GGCATGGTCG CCCTGGGTGA CACCGTGGAG 
GAAGCTTTCT ACAAGGTCTT CCATCTGCAG GCTGCGTGTG AGGTACAGGT GTCGGCTCTG 
TCCAGTGCTG GGGGCACTGA GAACCTCATC CTCTTGGAGC AAGAGAAACA CCGGCCGCAC 
GAGGTGGGCT CTGTGCAGTG GGCCGGCAGC ACCTTCGGGC CCATGCAGAA GAGCCGGCTG 
GGAGAGCATG AATTTGAAGC CCTCATGAGG ATGCTGGACA ATTTAGGCTA CAGAACAGGC 
TATACGTACC GCCACCCCTT TGTCCAAGAG AAAACCAAAC ACAAAAGTGA AGTGGAGATC 
CCAGCCACAG TCACTGCCTT TGTGTTTGAA GAGGATGGAG TCCCAGTCCC CGCCCTGCGC 
CAGCACGCCC AGAAGCAGCA GAAGGAAAAG ACCCGCTGGC TTAACACTCC CAACACCTAC 
CTGCGGGTGA ACGTGGCTGA CGAGGTGCAG AGGAACATGG GCAGTCCCCG ACCAAAGACC 
ACGTGGATGA AGGCTGATGA AGTGGAAAAG TCCAGCAGCG GCATGCCCAT ACGGATTGAA 
AACCCAAACC AATTTGTGCC TCTCTACACT GACCCCCAGG AAGTTCTGGA CATGAGGAAC 
AAGATTCGAG AGCAAAACCG ACAAGACATA AAGTCAGCCG GGCCTCAGTC TCAGCTCTTG 
GCCAGTGTCA TCGCAGAGAA GAGCCGGAGT CCGGTACAGC AGAGACTGCC CCCAACCGAA 
GGGGAAGTGT ATCAGACTCC TGGGGCTGGG CAGGGGACCC CTGAGTCCTC AGGCCCGCTC 
ACCCCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Sez6l2 regulates phosphorylation of ADD and neuritogenesis.
Biochemical and biophysical research communications494(1-2)234-241(2017 12)
Yaguchi H,Yabe I,Takahashi H,Watanabe M,Nomura T,Kano T,Matsumoto M,Nakayama KI,Watanabe M,Hatakeyama S

Circulating primitive erythroblasts establish a functional, protein 4.1R-dependent cytoskeletal network prior to enucleating.
Scientific reports7(1)5164(2017 Jul)
Huang YS,Delgadillo LF,Cyr KH,Kingsley PD,An X,McGrath KE,Mohandas N,Conboy JG,Waugh RE,Wan J,Palis J

Acute drug-induced spine changes in the nucleus accumbens are dependent on ?-adducin.
Neuropharmacology110(Pt A)333-342(2016 Nov)
Engmann O,Giralt A,Girault JA

Impaired synaptic plasticity and learning in mice lacking beta-adducin, an actin-regulating protein.
The Journal of neuroscience : the official journal of the Society for Neuroscience25(8)2138-45(2005 Feb)
Rabenstein RL,Addy NA,Caldarone BJ,Asaka Y,Gruenbaum LM,Peters LL,Gilligan DM,Fitzsimonds RM,Picciotto MR

The mouse adducin gene family: alternative splicing and chromosomal localization.
Mammalian genome : official journal of the International Mammalian Genome Society11(1)16-23(2000 Jan)
Suriyapperuma SP,Lozovatsky L,Ciciotte SL,Peters LL,Gilligan DM

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Add2 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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