Actn3 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Actn3
Entrez Gene ID	11474
Full Name	actinin alpha 3
General protein information	Preferred Names actinin alpha 3 Names alpha-actinin-3 F-actin cross-linking protein alpha-actinin skeletal muscle
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 19 Map Location: 19 A\|19 4.12 cM
Summary	This gene encodes a member of the alpha-actin binding protein gene family. The encoded protein is primarily expressed in skeletal muscle and functions as a structural component of sarcomeric Z line. This protein is involved in crosslinking actin containing thin filaments. [provided by RefSeq, Sep 2015].

mRNA and Protein(s)

mRNA	Protein	Name
NM_013456.2	NP_038484.1	alpha-actinin-3

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	ACTN3	XP_005631486.1
Anopheles gambiae (African malaria mosquito)	ACTN_ANOGA	XP_321625.2
Bos taurus (cattle)	ACTN3	NP_001069625.1
Mus musculus (house mouse)	Actn3	NP_038484.1
Pan troglodytes (chimpanzee)	ACTN3	XP_003313215.1
	ACTN3	XP_001109839.1
Danio rerio (zebrafish)	actn3b	NP_991107.1
Drosophila melanogaster (fruit fly)	Actn	NP_477485.1
Rattus norvegicus (Norway rat)	Actn3	NP_596915.1
Danio rerio (zebrafish)	actn3a	NP_571597.1
Caenorhabditis elegans (roundworm)	atn-1	NP_506128.1
Xenopus tropicalis (tropical clawed frog)	actn3	NP_001135513.1

Gene Ontology
Bibliography

Related articles in PubMed

Exploring the relationship between ?-actinin-3 deficiency and obesity in mice and humans.
Houweling PJ, Berman YD, Turner N, Quinlan KGR, Seto JT, Yang N, Lek M, Macarthur DG, Cooney G, North KN
International journal of obesity (2005)41(7)1154-1157(2017 Jul)

Evidence for ACTN3 as a genetic modifier of Duchenne muscular dystrophy.
Hogarth MW, Houweling PJ, Thomas KC, Gordish-Dressman H, Bello L, , Pegoraro E, Hoffman EP, Head SI, North KN
Nature communications814143(2017 Jan)

Defective excitation-contraction coupling is partially responsible for impaired contractility in hindlimb muscles of Stac3 knockout mice.
Cong X, Doering J, Grange RW, Jiang H
Scientific reports626194(2016 May)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

New insights that have been made in understanding the molecular mechanisms that underlie the consequences of alpha-actinin-3 deficiency.[review]
Title: How does ?-actinin-3 deficiency alter muscle function? Mechanistic insights into ACTN3, the 'gene for speed'.

Actn3 controls sarcomeric composition and muscle function in a dose-dependent fashion.
Title: Analysis of the ACTN3 heterozygous genotype suggests that ?-actinin-3 controls sarcomeric composition and muscle function in a dose-dependent fashion.

Absence of alpha-actinin-3 resulted in reduced atrophic response and altered adaptation to disuse, as measured by a change in myosin heavy chain isoform.
Title: ?-Actinin-3 deficiency alters muscle adaptation in response to denervation and immobilization.

ACTN3 likely contributes to the regulation of bone mass through alterations in bone turnover.
Title: ?-Actinin-3 deficiency is associated with reduced bone mass in human and mouse.

Data demonstrated that the Z-disk proteins, ZASP, titin and vinculin preferentially bind to alpha-actinin-2. Thus, the loss of alpha-actinin-3 changes the overall protein composition of fast fiber Z-disks and alters their elastic properties.
Title: Deficiency of ?-actinin-3 is associated with increased susceptibility to contraction-induced damage and skeletal muscle remodeling.

The following Actn3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Actn3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu18266	NM_013456.2 Latest version!	Mus musculus actinin alpha 3 (Actn3), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$531.30 $759.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Actn3 ( NM_013456.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_038484.1 Mus musculus actinin alpha 3 (Actn3), mRNA.

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CloneID	OMu18266
Clone ID Related Accession (Same CDS sequence)	NM_013456.2
Accession Version	NM_013456.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2703bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-22
Organism	Mus musculus(house mouse)
Product	alpha-actinin-3
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BY132390.1, AF093775.1 and BX630701.1. On Jan 4, 2013 this sequence version replaced NM_013456.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF093775.1, BC111890.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGATGATGG TTATGCAGCC CGAGGGTCTG GGGGCCGGGG AGGGGCCCTT CTCTGGCGGT 
GGGGGCGGCG AGTACATGGA ACAGGAGGAG GACTGGGACC GCGACTTGCT GTTGGACCCG 
GCCTGGGAGA AACAGCAGCG GAAAACCTTC ACTGCCTGGT GCAACTCACA TCTGCGCAAG 
GCAGGCACGC AGATCGAGAA CATTGAAGAG GACTTCCGCA ATGGCCTGAA GCTCATGCTG 
CTCCTGGAGG TCATTTCCGG AGAGAGGCTG CCTAGGCCCG ACAAAGGCAA GATGCGCTTC 
CACAAAATCG CCAATGTCAA TAAAGCCCTA GACTTCATTG CCAGCAAAGG AGTTAAGCTA 
GTGTCCATCG GTGCTGAAGA AATTGTTGAC GGGAACCTGA AGATGACCCT GGGCATGATC 
TGGACCATCA TCCTCCGCTT TGCCATTCAG GACATCTCTG TAGAAGAGAC CTCAGCCAAA 
GAAGGCTTGC TTCTCTGGTG TCAGCGGAAA ACAGCACCGT ACCGCAATGT CAACGTACAG 
AACTTCCATA CCAGCTGGAA GGATGGCCTG GCCCTCTGTG CTCTCATCCA CCGTCACCGG 
CCAGACCTCA TTGACTATGC CAAGCTTCGC AAGGATGACC CAATTGGAAA CCTGAACACT 
GCCTTCGAGG TGGCAGAGAA ATACCTGGAC ATTCCTAAGA TGCTGGATGC AGAAGATATC 
GTGAACACCC CCAAACCAGA TGAGAAAGCC ATCATGACCT ATGTTTCCTG CTTCTACCAT 
GCGTTTGCTG GGGCTGAGCA GGCAGAGACT GCTGCCAACA GGATCTGCAA GGTCTTAGCT 
GTGAACCAGG AAAATGAGAA GCTGATGGAG GAGTATGAGA AACTGGCCAG TGAGCTGTTG 
GAGTGGATCC GCCGCACTGT CCCATGGCTA GAGAACCGCG TGGGCGAACC CAGCATGAGT 
GCCATGCAGC GCAAGCTGGA GGACTTCCGA GACTATCGAC GCCTGCACAA GCCTCCCCGT 
GTGCAGGAGA AGTGCCAGCT GGAGATCAAC TTCAACACGC TGCAGACCAA GTTGCGCCTG 
AGCCACCGAC CCGCTTTCAT GCCCTCCGAG GGCAAGCTGG TTTCGGACAT AGCCAATGCG 
TGGCGGGGAC TGGAGCAGGT AGAGAAGGGC TATGAGGATT GGCTGCTTTC GGAGATCAGG 
CGTCTGCAGA GGCTTCAGCA CCTGGCTGAG AAGTTCCAAC AGAAGGCTTC CCTGCATGAA 
GCTTGGACCC GGGGCAAAGA GGAAATGTTA AACCAGCACG ACTACGAGTC AGCCTCGCTG 
CAGGAGGTGC GTGCGCTCTT GCGACGTCAT GAGGCCTTTG AGAGCGACCT GGCTGCGCAC 
CAAGACCGGG TGGAACACAT CGCGGCCCTG GCCCAGGAAC TCAATGAGCT CGACTACCAT 
GAGGCAGCCT CCGTGAACAG CCGCTGCCAA GCCATCTGTG ACCAGTGGGA TAACTTGGGT 
ACACTGACCC AGAAGAGGAG GGACGCACTA GAGAGGATGG AGAAGCTCCT GGAGACCATT 
GACCAGCTGC AGCTGGAGTT TGCTCGGCGG GCAGCCCCCT TCAATAACTG GCTGGATGGG 
GCTATTGAGG ACCTGCAGGA TGTGTGGCTC GTGCACTCTG TGGAGGAGAC CCAGAGCCTG 
CTAACAGCAC ACGAACAGTT CAAGGCAACG TTGCCCGAGG CTGATCGAGA GCGAGGCGCC 
ATCCTGGGCA TCCAAGGAGA GATCCAGAAG ATTTGCCAAA CATACGGACT ACGGCCAAAG 
TCCGGCAACC CCTACATCAC CCTCAGTTCG CAGGACATCA ACAATAAGTG GGATACAGTC 
AGAAAGCTGG TACCCAGCCG TGACCAGACA CTGCAGGAGG AGCTGGCCCG GCAGCAGGTG 
AACGAGAGGC TCCGGCGACA GTTTGCAGCC CAGGCTAATG CCATAGGACC CTGGATCCAG 
GGAAAAGTGG AGGAAGTAGG ACGGCTGGCA GCTGGGCTGG CCGGCTCTCT GGAGGAGCAG 
ATGGCAGGTC TGCGGCAGCA GGAACAGAAC ATCATCAATT ACAAGAGCAA CATCGACCGG 
CTGGAGGGTG ACCACCAGCT GCTGCAGGAG AGCCTAGTCT TTGACAACAA GCACACAGTC 
TACAGCATGG AGCACATTCG TGTGGGCTGG GAGCAGTTGC TCACCTCCAT TGCCCGCACC 
ATCAATGAGG TCGAGAACCA GGTACTGACC CGAGATGCCA AGGGCCTGAG CCAGGAGCAG 
CTCAACGAAT TCCGCGCCTC CTTCAACCAC TTTGACCGGA AGCGGAATGG GATGATGGAG 
CCCGACGACT TCCGAGCTTG TCTCATCTCC ATGGGTTACG ATCTGGGAGA AGTGGAGTTT 
GCTCGAATCA TGACCATGGT GGACCCCAAT GCAGCTGGGG TTGTGACCTT CCAAGCCTTC 
ATTGACTTCA TGACCCGAGA GACTGCCGAG ACAGACACGG CTGAACAAGT GGTAGCCTCC 
TTCAAAATCC TGGCAGGAGA CAAGAACTAC ATTACCCCAG AGGAGCTGCG GAGAGAGCTC 
CCAGCCGAGC AGGCTGAGTA CTGCATCCGT CGGATGGCGC CCTACAAGGG ATCTGGGGCT 
CCATCTGGAG CTCTGGACTA CGTGGCTTTC TCTAGTGCCC TCTATGGAGA GAGTGACCTC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_038484.1
CDS	56..2758
Translation	MMMVMQPEGLGAGEGPFSGGGGGEYMEQEEDWDRDLLLDPAWEKQQRKTFTAWCNSHLRKAGTQIENIEEDFRNGLKLMLLLEVISGERLPRPDKGKMRFHKIANVNKALDFIASKGVKLVSIGAEEIVDGNLKMTLGMIWTIILRFAIQDISVEETSAKEGLLLWCQRKTAPYRNVNVQNFHTSWKDGLALCALIHRHRPDLIDYAKLRKDDPIGNLNTAFEVAEKYLDIPKMLDAEDIVNTPKPDEKAIMTYVSCFYHAFAGAEQAETAANRICKVLAVNQENEKLMEEYEKLASELLEWIRRTVPWLENRVGEPSMSAMQRKLEDFRDYRRLHKPPRVQEKCQLEINFNTLQTKLRLSHRPAFMPSEGKLVSDIANAWRGLEQVEKGYEDWLLSEIRRLQRLQHLAEKFQQKASLHEAWTRGKEEMLNQHDYESASLQEVRALLRRHEAFESDLAAHQDRVEHIAALAQELNELDYHEAASVNSRCQAICDQWDNLGTLTQKRRDALERMEKLLETIDQLQLEFARRAAPFNNWLDGAIEDLQDVWLVHSVEETQSLLTAHEQFKATLPEADRERGAILGIQGEIQKICQTYGLRPKSGNPYITLSSQDINNKWDTVRKLVPSRDQTLQEELARQQVNERLRRQFAAQANAIGPWIQGKVEEVGRLAAGLAGSLEEQMAGLRQQEQNIINYKSNIDRLEGDHQLLQESLVFDNKHTVYSMEHIRVGWEQLLTSIARTINEVENQVLTRDAKGLSQEQLNEFRASFNHFDRKRNGMMEPDDFRACLISMGYDLGEVEFARIMTMVDPNAAGVVTFQAFIDFMTRETAETDTAEQVVASFKILAGDKNYITPEELRRELPAEQAEYCIRRMAPYKGSGAPSGALDYVAFSSALYGESDL

Target ORF information:

RefSeq Version	NM_013456.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus actinin alpha 3 (Actn3), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_013456.2

ORF Insert Sequence:

ATGATGATGG TTATGCAGCC CGAGGGTCTG GGGGCCGGGG AGGGGCCCTT CTCTGGCGGT 
GGGGGCGGCG AGTACATGGA ACAGGAGGAG GACTGGGACC GCGACTTGCT GTTGGACCCG 
GCCTGGGAGA AACAGCAGCG GAAAACCTTC ACTGCCTGGT GCAACTCACA TCTGCGCAAG 
GCAGGCACGC AGATCGAGAA CATTGAAGAG GACTTCCGCA ATGGCCTGAA GCTCATGCTG 
CTCCTGGAGG TCATTTCCGG AGAGAGGCTG CCTAGGCCCG ACAAAGGCAA GATGCGCTTC 
CACAAAATCG CCAATGTCAA TAAAGCCCTA GACTTCATTG CCAGCAAAGG AGTTAAGCTA 
GTGTCCATCG GTGCTGAAGA AATTGTTGAC GGGAACCTGA AGATGACCCT GGGCATGATC 
TGGACCATCA TCCTCCGCTT TGCCATTCAG GACATCTCTG TAGAAGAGAC CTCAGCCAAA 
GAAGGCTTGC TTCTCTGGTG TCAGCGGAAA ACAGCACCGT ACCGCAATGT CAACGTACAG 
AACTTCCATA CCAGCTGGAA GGATGGCCTG GCCCTCTGTG CTCTCATCCA CCGTCACCGG 
CCAGACCTCA TTGACTATGC CAAGCTTCGC AAGGATGACC CAATTGGAAA CCTGAACACT 
GCCTTCGAGG TGGCAGAGAA ATACCTGGAC ATTCCTAAGA TGCTGGATGC AGAAGATATC 
GTGAACACCC CCAAACCAGA TGAGAAAGCC ATCATGACCT ATGTTTCCTG CTTCTACCAT 
GCGTTTGCTG GGGCTGAGCA GGCAGAGACT GCTGCCAACA GGATCTGCAA GGTCTTAGCT 
GTGAACCAGG AAAATGAGAA GCTGATGGAG GAGTATGAGA AACTGGCCAG TGAGCTGTTG 
GAGTGGATCC GCCGCACTGT CCCATGGCTA GAGAACCGCG TGGGCGAACC CAGCATGAGT 
GCCATGCAGC GCAAGCTGGA GGACTTCCGA GACTATCGAC GCCTGCACAA GCCTCCCCGT 
GTGCAGGAGA AGTGCCAGCT GGAGATCAAC TTCAACACGC TGCAGACCAA GTTGCGCCTG 
AGCCACCGAC CCGCTTTCAT GCCCTCCGAG GGCAAGCTGG TTTCGGACAT AGCCAATGCG 
TGGCGGGGAC TGGAGCAGGT AGAGAAGGGC TATGAGGATT GGCTGCTTTC GGAGATCAGG 
CGTCTGCAGA GGCTTCAGCA CCTGGCTGAG AAGTTCCAAC AGAAGGCTTC CCTGCATGAA 
GCTTGGACCC GGGGCAAAGA GGAAATGTTA AACCAGCACG ACTACGAGTC AGCCTCGCTG 
CAGGAGGTGC GTGCGCTCTT GCGACGTCAT GAGGCCTTTG AGAGCGACCT GGCTGCGCAC 
CAAGACCGGG TGGAACACAT CGCGGCCCTG GCCCAGGAAC TCAATGAGCT CGACTACCAT 
GAGGCAGCCT CCGTGAACAG CCGCTGCCAA GCCATCTGTG ACCAGTGGGA TAACTTGGGT 
ACACTGACCC AGAAGAGGAG GGACGCACTA GAGAGGATGG AGAAGCTCCT GGAGACCATT 
GACCAGCTGC AGCTGGAGTT TGCTCGGCGG GCAGCCCCCT TCAATAACTG GCTGGATGGG 
GCTATTGAGG ACCTGCAGGA TGTGTGGCTC GTGCACTCTG TGGAGGAGAC CCAGAGCCTG 
CTAACAGCAC ACGAACAGTT CAAGGCAACG TTGCCCGAGG CTGATCGAGA GCGAGGCGCC 
ATCCTGGGCA TCCAAGGAGA GATCCAGAAG ATTTGCCAAA CATACGGACT ACGGCCAAAG 
TCCGGCAACC CCTACATCAC CCTCAGTTCG CAGGACATCA ACAATAAGTG GGATACAGTC 
AGAAAGCTGG TACCCAGCCG TGACCAGACA CTGCAGGAGG AGCTGGCCCG GCAGCAGGTG 
AACGAGAGGC TCCGGCGACA GTTTGCAGCC CAGGCTAATG CCATAGGACC CTGGATCCAG 
GGAAAAGTGG AGGAAGTAGG ACGGCTGGCA GCTGGGCTGG CCGGCTCTCT GGAGGAGCAG 
ATGGCAGGTC TGCGGCAGCA GGAACAGAAC ATCATCAATT ACAAGAGCAA CATCGACCGG 
CTGGAGGGTG ACCACCAGCT GCTGCAGGAG AGCCTAGTCT TTGACAACAA GCACACAGTC 
TACAGCATGG AGCACATTCG TGTGGGCTGG GAGCAGTTGC TCACCTCCAT TGCCCGCACC 
ATCAATGAGG TCGAGAACCA GGTACTGACC CGAGATGCCA AGGGCCTGAG CCAGGAGCAG 
CTCAACGAAT TCCGCGCCTC CTTCAACCAC TTTGACCGGA AGCGGAATGG GATGATGGAG 
CCCGACGACT TCCGAGCTTG TCTCATCTCC ATGGGTTACG ATCTGGGAGA AGTGGAGTTT 
GCTCGAATCA TGACCATGGT GGACCCCAAT GCAGCTGGGG TTGTGACCTT CCAAGCCTTC 
ATTGACTTCA TGACCCGAGA GACTGCCGAG ACAGACACGG CTGAACAAGT GGTAGCCTCC 
TTCAAAATCC TGGCAGGAGA CAAGAACTAC ATTACCCCAG AGGAGCTGCG GAGAGAGCTC 
CCAGCCGAGC AGGCTGAGTA CTGCATCCGT CGGATGGCGC CCTACAAGGG ATCTGGGGCT 
CCATCTGGAG CTCTGGACTA CGTGGCTTTC TCTAGTGCCC TCTATGGAGA GAGTGACCTC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Exploring the relationship between ?-actinin-3 deficiency and obesity in mice and humans.
International journal of obesity (2005)41(7)1154-1157(2017 Jul)
Houweling PJ,Berman YD,Turner N,Quinlan KGR,Seto JT,Yang N,Lek M,Macarthur DG,Cooney G,North KN

Evidence for ACTN3 as a genetic modifier of Duchenne muscular dystrophy.
Nature communications814143(2017 Jan)
Hogarth MW,Houweling PJ,Thomas KC,Gordish-Dressman H,Bello L,,Pegoraro E,Hoffman EP,Head SI,North KN

Defective excitation-contraction coupling is partially responsible for impaired contractility in hindlimb muscles of Stac3 knockout mice.
Scientific reports626194(2016 May)
Cong X,Doering J,Grange RW,Jiang H

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Actn3 cDNA ORF clone, Mus musculus(house mouse)

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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