Tars cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Tars
Entrez Gene ID	110960
Full Name	threonyl-tRNA synthetase
Synonyms	D15Wsu59e,ThrRS
General protein information	Preferred Names threonyl-tRNA synthetase Names threonine--tRNA ligase, cytoplasmic threonyl-tRNA synthetase, cytoplasmic
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 15 Map Location: 15 A1\|15 5.6 cM

mRNA and Protein(s)

mRNA	Protein	Name
NM_033074.3	NP_149065.2	threonine--tRNA ligase 1, cytoplasmic

Pathways from BioSystems

Homologies

Danio rerio (zebrafish)	tars	NP_001116258.1
Drosophila melanogaster (fruit fly)	Aats-thr	NP_524839.3
Caenorhabditis elegans (roundworm)	tars-1	NP_001022033.1
Magnaporthe oryzae (rice blast fungus)	MGG_03551	XP_003716364.1
Arabidopsis thaliana (thale cress)	AT5G26830	NP_198035.2
Rattus norvegicus (Norway rat)	Tars	NP_001006977.1
Gallus gallus (chicken)	TARS	NP_001026618.1
Saccharomyces cerevisiae (baker's yeast)	THS1	NP_116578.3
Schizosaccharomyces pombe (fission yeast)	trs1	NP_596364.1
Canis lupus familiaris (dog)	TARS	XP_536509.3
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP008929	XP_319682.4
Eremothecium gossypii	AGOS_ACL039W	NP_983365.1
Neurospora crassa	NCU02380	XP_959801.1
Homo sapiens (human)	TARS	NP_001245367.1
Oryza sativa (rice)	Os08g0295300	NP_001061475.1
Xenopus tropicalis (tropical clawed frog)	tars	NP_001001219.1
Pan troglodytes (chimpanzee)	TARS	XP_003310808.1
Bos taurus (cattle)	TARS	NP_001029542.1
Mus musculus (house mouse)	Tars	NP_149065.2
Kluyveromyces lactis	KLLA0F05918g	XP_455348.1

Gene Ontology
Bibliography

Related articles in PubMed

BayGenomics: a resource of insertional mutations in mouse embryonic stem cells.
Stryke D, Kawamoto M, Huang CC, Johns SJ, King LA, Harper CA, Meng EC, Lee RE, Yee A, L'Italien L, Chuang PT, Young SG, Skarnes WC, Babbitt PC, Ferrin TE
Nucleic acids research31(1)278-81(2003 Jan)

p38 is essential for the assembly and stability of macromolecular tRNA synthetase complex: implications for its physiological significance.
Kim JY, Kang YS, Lee JW, Kim HJ, Ahn YH, Park H, Ko YG, Kim S
Proceedings of the National Academy of Sciences of the United States of America99(12)7912-6(2002 Jun)

Genome-wide mapping of unselected transcripts from extraembryonic tissue of 7.5-day mouse embryos reveals enrichment in the t-complex and under-representation on the X chromosome.
Ko MS, Threat TA, Wang X, Horton JH, Cui Y, Wang X, Pryor E, Paris J, Wells-Smith J, Kitchen JR, Rowe LB, Eppig J, Satoh T, Brant L, Fujiwara H, Yotsumoto S, Nakashima H
Human molecular genetics7(12)1967-78(1998 Nov)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Tars gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Tars cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu14955	NM_033074.3 Latest version!	Mus musculus threonyl-tRNA synthetase (Tars), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Tars ( NM_033074.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_149065.2 Mus musculus threonyl-tRNA synthetase (Tars), mRNA.

Price & Availability↑

CloneID	OMu14955
Clone ID Related Accession (Same CDS sequence)	NM_033074.3
Accession Version	NM_033074.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2169bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-14
Organism	Mus musculus(house mouse)
Product	threonine--tRNA ligase 1, cytoplasmic
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AK011146.2. On Apr 5, 2007 this sequence version replaced NM_033074.2. ##Evidence-Data-START## Transcript exon combination :: AK011146.2, AK167597.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164135, SAMN01164140 [ECO:0000348] ##Evidence-Data-END##

ATGTCGCAGG AGAAGGCTAG CAGCCCTTCG GGGAAGATGG ACGGCGAGAA GCCGGTGGAT 
GCCAGCGAGG AGAAGCGGAA AGAAGGAGGC AAGAAGAAGA GCAAAGATGG AGGTGGAGAT 
GGAGGTCGAG CAGAGTTGAA TCCCTGGCCT GAATACATTA ACACACGCCT TGATATGTAC 
AACAAACTCA AAGCAGAACA CGATTCTATC CTGGCCGAAA AGGCAGCAAA AGATAGCAAG 
CCGATTAAAG TCACCCTGCC TGACGGCAAG CAGGTGGATG CGGAGTCCTG GAAAACCACG 
CCGTATCAGA TTGCTTGTGG AATTAGTCAG GGCCTGGCTG ACAACACCGT TGTTGCTAAG 
GTGAACAAAG TCGTTTGGGA CCTGGACCGC CCGCTGGAGA CAGATTGCAC GTTGGAGCTG 
CTCAAGTTTG AGGACGAGGA AGCGCAGGCC GTATACTGGC ACTCCAGTGC CCACATAATG 
GGCGAGGCCA TGGAGAGAGT CTATGGCGGA TGTCTATGTT ACGGCCCACC AATAGAAAAC 
GGCTTCTATT ATGATATGTA CCTTGAAGAA GGGGGCGTGT CCAGCAATGA CTTCTCTTCC 
CTGGAAACTT TGTGCAAGAA AATCATTAAA GAGAAACAAA CTTTCGAAAG ATTGGAAGTT 
AAAAAGGAAA CATTACTAGA AATGTTTAAG TACAACAAGT TCAAGTGCCG GATATTGAAT 
GAGAAAGTGA ATACTCCGAC CACGACTGTC TACAGATGTG GCCCTCTGAT AGATCTCTGC 
CGGGGTCCTC ATGTCCGACA CACCGGCAAG ATTAAGACTT TGAAGATACA CAAGAATTCT 
TCCACGTACT GGGAAGGCAA AGCAGACATG GAGACGCTCC AGCGGATCTA TGGCATTTCA 
TTCCCTGACC CCAAGCTACT GAAAGAGTGG GAGAAGTTCC AAGAGGAAGC CAAAAACCGA 
GACCACAGGA AAATCGGGAG GGACCAAGAA CTATATTTCT TCCATGAACT TAGCCCTGGA 
AGTTGCTTTT TCCTGCCCAA AGGAGCCTAC ATTTATAATA CACTAATGGA ATTTATCAGG 
AGTGAATATA GGAAAAGAGG GTTCCAGGAG GTCGTCACTC CCAACATCTT CAATAGCCGG 
CTCTGGATGA CCTCTGGCCA CTGGCAGCAC TACAGCGAGA ACATGTTCTC CTTTGAGGTG 
GAGAAGGAGC AGTTTGCACT TAAGCCCATG AACTGCCCGG GACACTGCCT GATGTTCGAT 
CACCGGCCAC GGTCCTGGCG AGAGCTGCCC CTGCGGCTAG CTGACTTTGG TGTGCTGCAT 
AGGAATGAGC TCTCGGGGGC TCTCACCGGG CTCACACGGG TCCGAAGATT CCAGCAGGAT 
GACGCACACA TCTTCTGTGC CATGGAGCAG ATTGAAGATG AAATCAAAGG TTGTTTGGAT 
TTTCTTCGCA CAGTATATAG TGTCTTTGGA TTTTCATTTA AATTGAATCT TTCTACTCGC 
CCAGAAAAAT TCCTTGGAGA TATTGAAATA TGGAACCAAG CTGAGAAACA ACTTGAAAAC 
AGTTTGAATG AGTTCGGTGA GAAGTGGGAA CTAAATCCTG GAGATGGAGC CTTCTATGGG 
CCAAAGATTG ACATACAGAT AAAAGATGCC ATCGGTCGCT ACCACCAGTG TGCAACCATC 
CAGCTGGACT TTCAGTTGCC CATCAGGTTT AACCTTACTT ACGTCAGCCA CGATGGCGAT 
GATAAGAAAA GGCCAGTGAT TGTTCACAGA GCCATCCTGG GGTCGGTGGA AAGAATGATT 
GCCATCCTCA CAGAAAACTA TGGGGGCAAA TGGCCTTTCT GGCTCTCTCC TCGCCAGGTG 
ATGGTGGTCC CAGTGGGACC GACATGTGAT GAATATGCCC AAAAGGTACG GCAACAATTC 
CATGATGCTA AATTCATGGC GGACACTGAC CTGGATCCAG GCTGTACCTT GAATAAGAAG 
ATCAGAAACG CACAGTTAGC ACAGTATAAC TTCATCCTTG TTGTTGGTGA AAAAGAGAAA 
GCCAGTGGCA CGGTGAACAT CCGCACGAGA GACAATAAGG TCCACGGAGA GCGGACAGTG 
GAGGAGACGG TGCGGCGGCT GCAGCAGCTC AAGCAGACGC GCAGCAAGCA GGCAGAGGAG 
GAGTTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_149065.2
CDS	120..2288
Translation	MSQEKASSPSGKMDGEKPVDASEEKRKEGGKKKSKDGGGDGGRAELNPWPEYINTRLDMYNKLKAEHDSILAEKAAKDSKPIKVTLPDGKQVDAESWKTTPYQIACGISQGLADNTVVAKVNKVVWDLDRPLETDCTLELLKFEDEEAQAVYWHSSAHIMGEAMERVYGGCLCYGPPIENGFYYDMYLEEGGVSSNDFSSLETLCKKIIKEKQTFERLEVKKETLLEMFKYNKFKCRILNEKVNTPTTTVYRCGPLIDLCRGPHVRHTGKIKTLKIHKNSSTYWEGKADMETLQRIYGISFPDPKLLKEWEKFQEEAKNRDHRKIGRDQELYFFHELSPGSCFFLPKGAYIYNTLMEFIRSEYRKRGFQEVVTPNIFNSRLWMTSGHWQHYSENMFSFEVEKEQFALKPMNCPGHCLMFDHRPRSWRELPLRLADFGVLHRNELSGALTGLTRVRRFQQDDAHIFCAMEQIEDEIKGCLDFLRTVYSVFGFSFKLNLSTRPEKFLGDIEIWNQAEKQLENSLNEFGEKWELNPGDGAFYGPKIDIQIKDAIGRYHQCATIQLDFQLPIRFNLTYVSHDGDDKKRPVIVHRAILGSVERMIAILTENYGGKWPFWLSPRQVMVVPVGPTCDEYAQKVRQQFHDAKFMADTDLDPGCTLNKKIRNAQLAQYNFILVVGEKEKASGTVNIRTRDNKVHGERTVEETVRRLQQLKQTRSKQAEEEF

Target ORF information:

RefSeq Version	NM_033074.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus threonyl-tRNA synthetase (Tars), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_033074.3

ORF Insert Sequence:

ATGTCGCAGG AGAAGGCTAG CAGCCCTTCG GGGAAGATGG ACGGCGAGAA GCCGGTGGAT 
GCCAGCGAGG AGAAGCGGAA AGAAGGAGGC AAGAAGAAGA GCAAAGATGG AGGTGGAGAT 
GGAGGTCGAG CAGAGTTGAA TCCCTGGCCT GAATACATTA ACACACGCCT TGATATGTAC 
AACAAACTCA AAGCAGAACA CGATTCTATC CTGGCCGAAA AGGCAGCAAA AGATAGCAAG 
CCGATTAAAG TCACCCTGCC TGACGGCAAG CAGGTGGATG CGGAGTCCTG GAAAACCACG 
CCGTATCAGA TTGCTTGTGG AATTAGTCAG GGCCTGGCTG ACAACACCGT TGTTGCTAAG 
GTGAACAAAG TCGTTTGGGA CCTGGACCGC CCGCTGGAGA CAGATTGCAC GTTGGAGCTG 
CTCAAGTTTG AGGACGAGGA AGCGCAGGCC GTATACTGGC ACTCCAGTGC CCACATAATG 
GGCGAGGCCA TGGAGAGAGT CTATGGCGGA TGTCTATGTT ACGGCCCACC AATAGAAAAC 
GGCTTCTATT ATGATATGTA CCTTGAAGAA GGGGGCGTGT CCAGCAATGA CTTCTCTTCC 
CTGGAAACTT TGTGCAAGAA AATCATTAAA GAGAAACAAA CTTTCGAAAG ATTGGAAGTT 
AAAAAGGAAA CATTACTAGA AATGTTTAAG TACAACAAGT TCAAGTGCCG GATATTGAAT 
GAGAAAGTGA ATACTCCGAC CACGACTGTC TACAGATGTG GCCCTCTGAT AGATCTCTGC 
CGGGGTCCTC ATGTCCGACA CACCGGCAAG ATTAAGACTT TGAAGATACA CAAGAATTCT 
TCCACGTACT GGGAAGGCAA AGCAGACATG GAGACGCTCC AGCGGATCTA TGGCATTTCA 
TTCCCTGACC CCAAGCTACT GAAAGAGTGG GAGAAGTTCC AAGAGGAAGC CAAAAACCGA 
GACCACAGGA AAATCGGGAG GGACCAAGAA CTATATTTCT TCCATGAACT TAGCCCTGGA 
AGTTGCTTTT TCCTGCCCAA AGGAGCCTAC ATTTATAATA CACTAATGGA ATTTATCAGG 
AGTGAATATA GGAAAAGAGG GTTCCAGGAG GTCGTCACTC CCAACATCTT CAATAGCCGG 
CTCTGGATGA CCTCTGGCCA CTGGCAGCAC TACAGCGAGA ACATGTTCTC CTTTGAGGTG 
GAGAAGGAGC AGTTTGCACT TAAGCCCATG AACTGCCCGG GACACTGCCT GATGTTCGAT 
CACCGGCCAC GGTCCTGGCG AGAGCTGCCC CTGCGGCTAG CTGACTTTGG TGTGCTGCAT 
AGGAATGAGC TCTCGGGGGC TCTCACCGGG CTCACACGGG TCCGAAGATT CCAGCAGGAT 
GACGCACACA TCTTCTGTGC CATGGAGCAG ATTGAAGATG AAATCAAAGG TTGTTTGGAT 
TTTCTTCGCA CAGTATATAG TGTCTTTGGA TTTTCATTTA AATTGAATCT TTCTACTCGC 
CCAGAAAAAT TCCTTGGAGA TATTGAAATA TGGAACCAAG CTGAGAAACA ACTTGAAAAC 
AGTTTGAATG AGTTCGGTGA GAAGTGGGAA CTAAATCCTG GAGATGGAGC CTTCTATGGG 
CCAAAGATTG ACATACAGAT AAAAGATGCC ATCGGTCGCT ACCACCAGTG TGCAACCATC 
CAGCTGGACT TTCAGTTGCC CATCAGGTTT AACCTTACTT ACGTCAGCCA CGATGGCGAT 
GATAAGAAAA GGCCAGTGAT TGTTCACAGA GCCATCCTGG GGTCGGTGGA AAGAATGATT 
GCCATCCTCA CAGAAAACTA TGGGGGCAAA TGGCCTTTCT GGCTCTCTCC TCGCCAGGTG 
ATGGTGGTCC CAGTGGGACC GACATGTGAT GAATATGCCC AAAAGGTACG GCAACAATTC 
CATGATGCTA AATTCATGGC GGACACTGAC CTGGATCCAG GCTGTACCTT GAATAAGAAG 
ATCAGAAACG CACAGTTAGC ACAGTATAAC TTCATCCTTG TTGTTGGTGA AAAAGAGAAA 
GCCAGTGGCA CGGTGAACAT CCGCACGAGA GACAATAAGG TCCACGGAGA GCGGACAGTG 
GAGGAGACGG TGCGGCGGCT GCAGCAGCTC AAGCAGACGC GCAGCAAGCA GGCAGAGGAG 
GAGTTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

BayGenomics: a resource of insertional mutations in mouse embryonic stem cells.
Nucleic acids research31(1)278-81(2003 Jan)
Stryke D,Kawamoto M,Huang CC,Johns SJ,King LA,Harper CA,Meng EC,Lee RE,Yee A,L'Italien L,Chuang PT,Young SG,Skarnes WC,Babbitt PC,Ferrin TE

p38 is essential for the assembly and stability of macromolecular tRNA synthetase complex: implications for its physiological significance.
Proceedings of the National Academy of Sciences of the United States of America99(12)7912-6(2002 Jun)
Kim JY,Kang YS,Lee JW,Kim HJ,Ahn YH,Park H,Ko YG,Kim S

Genome-wide mapping of unselected transcripts from extraembryonic tissue of 7.5-day mouse embryos reveals enrichment in the t-complex and under-representation on the X chromosome.
Human molecular genetics7(12)1967-78(1998 Nov)
Ko MS,Threat TA,Wang X,Horton JH,Cui Y,Wang X,Pryor E,Paris J,Wells-Smith J,Kitchen JR,Rowe LB,Eppig J,Satoh T,Brant L,Fujiwara H,Yotsumoto S,Nakashima H

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Tars cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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