Pcca cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Pcca
Entrez Gene ID	110821
Full Name	propionyl-Coenzyme A carboxylase, alpha polypeptide
Synonyms	C79630
General protein information	Preferred Names propionyl-Coenzyme A carboxylase, alpha polypeptide Names propionyl-CoA carboxylase alpha chain, mitochondrial PCCase subunit alpha propanoyl-CoA:carbon dioxide ligase subunit alpha propionyl CoA-carboxylase alpha
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 14 Map Location: 14 E5\|14 65.99 cM

mRNA and Protein(s)

mRNA	Protein	Name
NM_144844.2	NP_659093.2	propionyl-CoA carboxylase alpha chain, mitochondrial precursor
XM_006518432.3	XP_006518495.1	propionyl-CoA carboxylase alpha chain, mitochondrial isoform X2
XM_006518430.4	XP_006518493.1	propionyl-CoA carboxylase alpha chain, mitochondrial isoform X1
XM_011244922.2	XP_011243224.1	propionyl-CoA carboxylase alpha chain, mitochondrial isoform X4
XM_030247609.1	XP_030103469.1	propionyl-CoA carboxylase alpha chain, mitochondrial isoform X3
XM_006518430.3	XP_006518493.1	propionyl-CoA carboxylase alpha chain, mitochondrial isoform X1

Pathways from BioSystems

Homologies

Gallus gallus (chicken)	PCCA	XP_416970.2
Rattus norvegicus (Norway rat)	Pcca	NP_062203.1
Caenorhabditis elegans (roundworm)	pcca-1	NP_509293.1
Xenopus tropicalis (tropical clawed frog)	pcca	XP_002937132.1
Pan troglodytes (chimpanzee)	PCCA	XP_001149000.1
Canis lupus familiaris (dog)	PCCA	XP_534175.2
Homo sapiens (human)	PCCA	NP_000273.2
	PCCA	XP_001092670.1
Mus musculus (house mouse)	Pcca	NP_659093.2
Danio rerio (zebrafish)	pcca	NP_001002746.1
Bos taurus (cattle)	PCCA	NP_001076978.1

Gene Ontology
Bibliography

Related articles in PubMed

Dysregulated miRNAs and their pathogenic implications for the neurometabolic disease propionic acidemia.
Rivera-Barahona A, Fulgencio-Covi?n A, P?rez-Cerd? C, Ramos R, Barry MA, Ugarte M, P?rez B, Richard E, Desviat LR
Scientific reports7(1)5727(2017 Jul)

CLUH regulates mitochondrial metabolism by controlling translation and decay of target mRNAs.
Schatton D, Pla-Martin D, Marx MC, Hansen H, Mourier A, Nemazanyy I, Pessia A, Zentis P, Corona T, Kondylis V, Barth E, Schauss AC, Velagapudi V, Rugarli EI
The Journal of cell biology216(3)675-693(2017 Mar)

In vivo evidence of mitochondrial dysfunction and altered redox homeostasis in a genetic mouse model of propionic acidemia: Implications for the pathophysiology of this disorder.
Gallego-Villar L, Rivera-Barahona A, Cuevas-Mart?n C, Guenzel A, P?rez B, Barry MA, Murphy MP, Logan A, Gonzalez-Quintana A, Mart?n MA, Medina S, Gil-Izquierdo A, Cuezva JM, Richard E, Desviat LR
Free radical biology & medicine961-12(2016 07)

Long-term sex-biased correction of circulating propionic acidemia disease markers by adeno-associated virus vectors.
Guenzel AJ, Collard R, Kraus JP, Matern D, Barry MA
Human gene therapy26(3)153-60(2015 Mar)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

we have investigated mitochondrial function, superoxide anion and H2O2 levels and oxidative damage in relevant tissues of the hypomorphic mice(knockout mouse model for the Pcca gene), to gain insight into the pathophysiological processes contributing to the multiorgan complications observed in propionic acidemia
Title: In vivo evidence of mitochondrial dysfunction and altered redox homeostasis in a genetic mouse model of propionic acidemia: Implications for the pathophysiology of this disorder.

A single intravenous injection of adeno-associated virus 2/8 or AAVrh10 expressing PCCA into propionic academia hypomorphic mice decreased systemic propionylcarnitine and methyl citrate for up to 1.5 years.
Title: Long-term sex-biased correction of circulating propionic acidemia disease markers by adeno-associated virus vectors.

The following Pcca gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Pcca cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu49589	XM_006518432.3 Latest version!	Mus musculus propionyl-Coenzyme A carboxylase, alpha polypeptide (Pcca), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$307.30 $439.00
OMu72371	XM_011244922.2 Latest version!	Mus musculus propionyl-Coenzyme A carboxylase, alpha polypeptide (Pcca), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
OMu15267	NM_144844.2 Latest version!	Mus musculus propionyl-Coenzyme A carboxylase, alpha polypeptide (Pcca), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$391.30 $559.00
OMu49587	XM_006518430.4 Latest version!	Mus musculus propionyl-Coenzyme A carboxylase, alpha polypeptide (Pcca), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OMu49590	XM_030247609.1 Latest version!	Mus musculus propionyl-Coenzyme A carboxylase, alpha polypeptide (Pcca), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
View Old Accession Versions >>
OMu49587	XM_006518430.3	Mus musculus propionyl-Coenzyme A carboxylase, alpha polypeptide (Pcca), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
Hide Old Accession Versions >>

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***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Pcca ( XM_006518432.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006518495.1 Mus musculus propionyl-Coenzyme A carboxylase, alpha polypeptide (Pcca), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OMu49589
Clone ID Related Accession (Same CDS sequence)	XM_006518432.3
Accession Version	XM_006518432.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1935bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	propionyl-CoA carboxylase alpha chain, mitochondrial isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000080.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006518432.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGCATCA AGACAGTTGC CATTCACAGT GATGTTGATG CCAGTTCTGT TCACGTGAAA 
ATGGCGGATG AGGCTGTCTG TGTTGGCCCA GCTCCCACCA GTAAAAGCTA CCTCAACATG 
GATGCCATCA TGGAAGCCAT TAAGAAAACC AGGGCCCAAG CTGTACACCC AGGGTATGGA 
TTCCTGTCAG AAAACAAAGA GTTTGCAAAG CGTCTGGCAG CAGAAGATGT CACTTTCATT 
GGACCTGATA CTCATGCTAT TCAAGCCATG GGTGACAAGA TAGAAAGCAA ACTATTAGCC 
AAGAGAGCAA AGGTCAACAC AATCCCTGGT TTTGATGGGG TAGTAAAGGA TGCAGATGAA 
GCTGTCAGAA TTGCAAGGGA AATTGGCTAC CCTGTGATGA TCAAGGCCTC AGCAGGCGGT 
GGTGGGAAAG GCATGCGCAT CGCCTGGGAT GACGAAGAGA CCAGGGATGG CTTTAGATTT 
TCATCCCAGG AAGCTGCTTC TAGTTTTGGT GATGATAGAC TACTAATAGA AAAATTTATC 
GATAACCCTC GTCATATAGA AATCCAGGTT TTAGGGGATA AACATGGCAA TGCTCTGTGG 
CTCAATGAGA GGGAGTGCTC GATCCAGAGA AGGAACCAGA AGGTGGTAGA GGAGGCGCCA 
AGCATTTTTC TGGATCCTGA AACTCGCCAA GCAATGGGAG AGCAGGCCGT GGCTTTGGCT 
AAAGCCGTGA AGTATTCTTC TGCTGGAACT GTGGAATTTC TTGTGGACTC CCAGAAGAAT 
TTTTACTTCT TGGAGATGAA TACAAGACTA CAGGTCGAAC ATCCTGTCAC AGAGTGCATT 
ACTGGCCTGG ACTTAGTCCA AGAAATGATC CTTGTTGCTA AGGGTTACCC ACTCAGGCAC 
AAGCAAGAGG ATATTCCCAT CAGTGGCTGG GCAGTTGAAT GTCGGGTTTA TGCTGAGGAC 
CCCTACAAGT CTTTCGGTTT ACCGTCTATT GGGAGGCTGT CCCAGTACCA AGAGCCGATA 
CATCTACCTG GTGTCCGAGT TGACAGTGGC ATCCAACCAG GAAGTGACAT CAGCATCTAT 
TATGATCCTA TGATTTCAAA GCTAGTCACA TATGGGTCTG ACAGAGCAGA AGCCCTGAAG 
AGGATGGAAG ACGCACTGGA CAATTATGTG ATCCGGGGTG TTACACACAA CATCCCATTG 
CTCCGGGAGG TGATAATCAA CACACGTTTT GTGAAAGGAG ACATCAGCAC TAAGTTTCTC 
TCTGATGTGT ATCCTGATGG CTTCAAAGGG CACACGTTAA CACTGAGTGA GAGAAACCAG 
TTATTGGCCA TTGCATCATC TGTATTTGTG GCATCCCAGC TACGAGCTCA GCGCTTCCAA 
GAACATTCAA GAGTACCAGT TATTAGGCCT GATGTGGCTA AGTGGGAGCT CTCGGTAAAG 
TTACATGATG AAGATCATAC TGTCGTGGCA TCTAACAATG GGCCGGCATT TACCGTGGAA 
GTTGATGGCT CGAAACTAAA TGTGACCAGT ACGTGGAACC TGGCGTCACC CTTATTGTCT 
GTCAACGTTG ATGGCACGCA GAGGACTGTG CAGTGTCTTT CTCGGGAAGC AGGTGGAAAC 
ATGAGCATCC AGTTTCTTGG CACAGTGTAC AAAGTGCACA TTTTAACCAA GCTTGCTGCA 
GAGCTGAACA AATTCATGCT TGAAAAAGTG CCCAAGGACA CCAGCAGCAC TCTGTGCTCC 
CCGATGCCTG GAGTGGTGGT GGCCGTTTCT GTCAAGCCTG GAGACATGGT AGCAGAAGGT 
CAGGAAATCT GTGTGATTGA AGCTATGAAA ATGCAGAACA GTATGACAGC TGGGAAAATG 
GGCAAGGTGA AATTGGTGCA CTGCAAAGCT GGAGACACAG TTGGTGAAGG AGACCTGCTT 
GTGGAGCTGG AATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006518495.1
CDS	230..2164
Translation	MGIKTVAIHSDVDASSVHVKMADEAVCVGPAPTSKSYLNMDAIMEAIKKTRAQAVHPGYGFLSENKEFAKRLAAEDVTFIGPDTHAIQAMGDKIESKLLAKRAKVNTIPGFDGVVKDADEAVRIAREIGYPVMIKASAGGGGKGMRIAWDDEETRDGFRFSSQEAASSFGDDRLLIEKFIDNPRHIEIQVLGDKHGNALWLNERECSIQRRNQKVVEEAPSIFLDPETRQAMGEQAVALAKAVKYSSAGTVEFLVDSQKNFYFLEMNTRLQVEHPVTECITGLDLVQEMILVAKGYPLRHKQEDIPISGWAVECRVYAEDPYKSFGLPSIGRLSQYQEPIHLPGVRVDSGIQPGSDISIYYDPMISKLVTYGSDRAEALKRMEDALDNYVIRGVTHNIPLLREVIINTRFVKGDISTKFLSDVYPDGFKGHTLTLSERNQLLAIASSVFVASQLRAQRFQEHSRVPVIRPDVAKWELSVKLHDEDHTVVASNNGPAFTVEVDGSKLNVTSTWNLASPLLSVNVDGTQRTVQCLSREAGGNMSIQFLGTVYKVHILTKLAAELNKFMLEKVPKDTSSTLCSPMPGVVVAVSVKPGDMVAEGQEICVIEAMKMQNSMTAGKMGKVKLVHCKAGDTVGEGDLLVELE

Target ORF information:

RefSeq Version	XM_006518432.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus propionyl-Coenzyme A carboxylase, alpha polypeptide (Pcca), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006518432.3

ORF Insert Sequence:

ATGGGCATCA AGACAGTTGC CATTCACAGT GATGTTGATG CCAGTTCTGT TCACGTGAAA 
ATGGCGGATG AGGCTGTCTG TGTTGGCCCA GCTCCCACCA GTAAAAGCTA CCTCAACATG 
GATGCCATCA TGGAAGCCAT TAAGAAAACC AGGGCCCAAG CTGTACACCC AGGGTATGGA 
TTCCTGTCAG AAAACAAAGA GTTTGCAAAG CGTCTGGCAG CAGAAGATGT CACTTTCATT 
GGACCTGATA CTCATGCTAT TCAAGCCATG GGTGACAAGA TAGAAAGCAA ACTATTAGCC 
AAGAGAGCAA AGGTCAACAC AATCCCTGGT TTTGATGGGG TAGTAAAGGA TGCAGATGAA 
GCTGTCAGAA TTGCAAGGGA AATTGGCTAC CCTGTGATGA TCAAGGCCTC AGCAGGCGGT 
GGTGGGAAAG GCATGCGCAT CGCCTGGGAT GACGAAGAGA CCAGGGATGG CTTTAGATTT 
TCATCCCAGG AAGCTGCTTC TAGTTTTGGT GATGATAGAC TACTAATAGA AAAATTTATC 
GATAACCCTC GTCATATAGA AATCCAGGTT TTAGGGGATA AACATGGCAA TGCTCTGTGG 
CTCAATGAGA GGGAGTGCTC GATCCAGAGA AGGAACCAGA AGGTGGTAGA GGAGGCGCCA 
AGCATTTTTC TGGATCCTGA AACTCGCCAA GCAATGGGAG AGCAGGCCGT GGCTTTGGCT 
AAAGCCGTGA AGTATTCTTC TGCTGGAACT GTGGAATTTC TTGTGGACTC CCAGAAGAAT 
TTTTACTTCT TGGAGATGAA TACAAGACTA CAGGTCGAAC ATCCTGTCAC AGAGTGCATT 
ACTGGCCTGG ACTTAGTCCA AGAAATGATC CTTGTTGCTA AGGGTTACCC ACTCAGGCAC 
AAGCAAGAGG ATATTCCCAT CAGTGGCTGG GCAGTTGAAT GTCGGGTTTA TGCTGAGGAC 
CCCTACAAGT CTTTCGGTTT ACCGTCTATT GGGAGGCTGT CCCAGTACCA AGAGCCGATA 
CATCTACCTG GTGTCCGAGT TGACAGTGGC ATCCAACCAG GAAGTGACAT CAGCATCTAT 
TATGATCCTA TGATTTCAAA GCTAGTCACA TATGGGTCTG ACAGAGCAGA AGCCCTGAAG 
AGGATGGAAG ACGCACTGGA CAATTATGTG ATCCGGGGTG TTACACACAA CATCCCATTG 
CTCCGGGAGG TGATAATCAA CACACGTTTT GTGAAAGGAG ACATCAGCAC TAAGTTTCTC 
TCTGATGTGT ATCCTGATGG CTTCAAAGGG CACACGTTAA CACTGAGTGA GAGAAACCAG 
TTATTGGCCA TTGCATCATC TGTATTTGTG GCATCCCAGC TACGAGCTCA GCGCTTCCAA 
GAACATTCAA GAGTACCAGT TATTAGGCCT GATGTGGCTA AGTGGGAGCT CTCGGTAAAG 
TTACATGATG AAGATCATAC TGTCGTGGCA TCTAACAATG GGCCGGCATT TACCGTGGAA 
GTTGATGGCT CGAAACTAAA TGTGACCAGT ACGTGGAACC TGGCGTCACC CTTATTGTCT 
GTCAACGTTG ATGGCACGCA GAGGACTGTG CAGTGTCTTT CTCGGGAAGC AGGTGGAAAC 
ATGAGCATCC AGTTTCTTGG CACAGTGTAC AAAGTGCACA TTTTAACCAA GCTTGCTGCA 
GAGCTGAACA AATTCATGCT TGAAAAAGTG CCCAAGGACA CCAGCAGCAC TCTGTGCTCC 
CCGATGCCTG GAGTGGTGGT GGCCGTTTCT GTCAAGCCTG GAGACATGGT AGCAGAAGGT 
CAGGAAATCT GTGTGATTGA AGCTATGAAA ATGCAGAACA GTATGACAGC TGGGAAAATG 
GGCAAGGTGA AATTGGTGCA CTGCAAAGCT GGAGACACAG TTGGTGAAGG AGACCTGCTT 
GTGGAGCTGG AATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Pcca ( XM_011244922.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011243224.1 Mus musculus propionyl-Coenzyme A carboxylase, alpha polypeptide (Pcca), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OMu72371
Clone ID Related Accession (Same CDS sequence)	XM_011244922.2
Accession Version	XM_011244922.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1173bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	propionyl-CoA carboxylase alpha chain, mitochondrial isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000080.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_011244922.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGAAAC CCTGTTTTAT AGGAGACGAC AGTTCCCTCC TATTTTATCG GGTCGAACAT 
CCTGTCACAG AGTGCATTAC TGGCCTGGAC TTAGTCCAAG AAATGATCCT TGTTGCTAAG 
GGTTACCCAC TCAGGCACAA GCAAGAGGAT ATTCCCATCA GTGGCTGGGC AGTTGAATGT 
CGGGTTTATG CTGAGGACCC CTACAAGTCT TTCGGTTTAC CGTCTATTGG GAGGCTGTCC 
CAGTACCAAG AGCCGATACA TCTACCTGGT GTCCGAGTTG ACAGTGGCAT CCAACCAGGA 
AGTGACATCA GCATCTATTA TGATCCTATG ATTTCAAAGC TAGTCACATA TGGGTCTGAC 
AGAGCAGAAG CCCTGAAGAG GATGGAAGAC GCACTGGACA ATTATGTGAT CCGGGGTGTT 
ACACACAACA TCCCATTGCT CCGGGAGGTG ATAATCAACA CACGTTTTGT GAAAGGAGAC 
ATCAGCACTA AGTTTCTCTC TGATGTGTAT CCTGATGGCT TCAAAGGGCA CACGTTAACA 
CTGAGTGAGA GAAACCAGTT ATTGGCCATT GCATCATCTG TATTTGTGGC ATCCCAGCTA 
CGAGCTCAGC GCTTCCAAGA ACATTCAAGA GTACCAGTTA TTAGGCCTGA TGTGGCTAAG 
TGGGAGCTCT CGGTAAAGTT ACATGATGAA GATCATACTG TCGTGGCATC TAACAATGGG 
CCGGCATTTA CCGTGGAAGT TGATGGCTCG AAACTAAATG TGACCAGTAC GTGGAACCTG 
GCGTCACCCT TATTGTCTGT CAACGTTGAT GGCACGCAGA GGACTGTGCA GTGTCTTTCT 
CGGGAAGCAG GTGGAAACAT GAGCATCCAG TTTCTTGGCA CAGTGTACAA AGTGCACATT 
TTAACCAAGC TTGCTGCAGA GCTGAACAAA TTCATGCTTG AAAAAGTGCC CAAGGACACC 
AGCAGCACTC TGTGCTCCCC GATGCCTGGA GTGGTGGTGG CCGTTTCTGT CAAGCCTGGA 
GACATGGTAG CAGAAGGTCA GGAAATCTGT GTGATTGAAG CTATGAAAAT GCAGAACAGT 
ATGACAGCTG GGAAAATGGG CAAGGTGAAA TTGGTGCACT GCAAAGCTGG AGACACAGTT 
GGTGAAGGAG ACCTGCTTGT GGAGCTGGAA TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011243224.1
CDS	575..1747
Translation	MMKPCFIGDDSSLLFYRVEHPVTECITGLDLVQEMILVAKGYPLRHKQEDIPISGWAVECRVYAEDPYKSFGLPSIGRLSQYQEPIHLPGVRVDSGIQPGSDISIYYDPMISKLVTYGSDRAEALKRMEDALDNYVIRGVTHNIPLLREVIINTRFVKGDISTKFLSDVYPDGFKGHTLTLSERNQLLAIASSVFVASQLRAQRFQEHSRVPVIRPDVAKWELSVKLHDEDHTVVASNNGPAFTVEVDGSKLNVTSTWNLASPLLSVNVDGTQRTVQCLSREAGGNMSIQFLGTVYKVHILTKLAAELNKFMLEKVPKDTSSTLCSPMPGVVVAVSVKPGDMVAEGQEICVIEAMKMQNSMTAGKMGKVKLVHCKAGDTVGEGDLLVELE

Target ORF information:

RefSeq Version	XM_011244922.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus propionyl-Coenzyme A carboxylase, alpha polypeptide (Pcca), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011244922.2

ORF Insert Sequence:

ATGATGAAAC CCTGTTTTAT AGGAGACGAC AGTTCCCTCC TATTTTATCG GGTCGAACAT 
CCTGTCACAG AGTGCATTAC TGGCCTGGAC TTAGTCCAAG AAATGATCCT TGTTGCTAAG 
GGTTACCCAC TCAGGCACAA GCAAGAGGAT ATTCCCATCA GTGGCTGGGC AGTTGAATGT 
CGGGTTTATG CTGAGGACCC CTACAAGTCT TTCGGTTTAC CGTCTATTGG GAGGCTGTCC 
CAGTACCAAG AGCCGATACA TCTACCTGGT GTCCGAGTTG ACAGTGGCAT CCAACCAGGA 
AGTGACATCA GCATCTATTA TGATCCTATG ATTTCAAAGC TAGTCACATA TGGGTCTGAC 
AGAGCAGAAG CCCTGAAGAG GATGGAAGAC GCACTGGACA ATTATGTGAT CCGGGGTGTT 
ACACACAACA TCCCATTGCT CCGGGAGGTG ATAATCAACA CACGTTTTGT GAAAGGAGAC 
ATCAGCACTA AGTTTCTCTC TGATGTGTAT CCTGATGGCT TCAAAGGGCA CACGTTAACA 
CTGAGTGAGA GAAACCAGTT ATTGGCCATT GCATCATCTG TATTTGTGGC ATCCCAGCTA 
CGAGCTCAGC GCTTCCAAGA ACATTCAAGA GTACCAGTTA TTAGGCCTGA TGTGGCTAAG 
TGGGAGCTCT CGGTAAAGTT ACATGATGAA GATCATACTG TCGTGGCATC TAACAATGGG 
CCGGCATTTA CCGTGGAAGT TGATGGCTCG AAACTAAATG TGACCAGTAC GTGGAACCTG 
GCGTCACCCT TATTGTCTGT CAACGTTGAT GGCACGCAGA GGACTGTGCA GTGTCTTTCT 
CGGGAAGCAG GTGGAAACAT GAGCATCCAG TTTCTTGGCA CAGTGTACAA AGTGCACATT 
TTAACCAAGC TTGCTGCAGA GCTGAACAAA TTCATGCTTG AAAAAGTGCC CAAGGACACC 
AGCAGCACTC TGTGCTCCCC GATGCCTGGA GTGGTGGTGG CCGTTTCTGT CAAGCCTGGA 
GACATGGTAG CAGAAGGTCA GGAAATCTGT GTGATTGAAG CTATGAAAAT GCAGAACAGT 
ATGACAGCTG GGAAAATGGG CAAGGTGAAA TTGGTGCACT GCAAAGCTGG AGACACAGTT 
GGTGAAGGAG ACCTGCTTGT GGAGCTGGAA TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Pcca ( XM_006518430.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006518493.1 Mus musculus propionyl-Coenzyme A carboxylase, alpha polypeptide (Pcca), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu49587
Clone ID Related Accession (Same CDS sequence)	XM_006518430.4 , XM_006518430.3
Accession Version	XM_006518430.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2121bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	propionyl-CoA carboxylase alpha chain, mitochondrial isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000080.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006518430.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGGGC AGTGGGTCAG GACCGTGGCG CTGTTGGCGG CCAGGCGGCA TTGGCGGCGG 
TCCTCGCAGC AGCAATTGCT GGGGACGCTG AAGCATGCTC CAGTCTATTC ATACCAATGC 
CTAGTGGTGT CCAGAAGTCT CAGTTCTGTG GAATATGAGC CTAAAGAAAA GACTTTTGAT 
AAAATTCTCA TTGCTAACAG AGGAGAAATT GCCTGTAGGG TTATTAAAAC TTGCAAGAAG 
ATGGGCATCA AGACAGTTGC CATTCACAGT GATGTTGATG CCAGTTCTGT TCACGTGAAA 
ATGGCGGATG AGGCTGTCTG TGTTGGCCCA GCTCCCACCA GTAAAAGCTA CCTCAACATG 
GATGCCATCA TGGAAGCCAT TAAGAAAACC AGGGCCCAAG CTGTACACCC AGGGTATGGA 
TTCCTGTCAG AAAACAAAGA GTTTGCAAAG CGTCTGGCAG CAGAAGATGT CACTTTCATT 
GGACCTGATA CTCATGCTAT TCAAGCCATG GGTGACAAGA TAGAAAGCAA ACTATTAGCC 
AAGAGAGCAA AGGTCAACAC AATCCCTGGT TTTGATGGGG TAGTAAAGGA TGCAGATGAA 
GCTGTCAGAA TTGCAAGGGA AATTGGCTAC CCTGTGATGA TCAAGGCCTC AGCAGGCGGT 
GGTGGGAAAG GCATGCGCAT CGCCTGGGAT GACGAAGAGA CCAGGGATGG CTTTAGATTT 
TCATCCCAGG AAGCTGCTTC TAGTTTTGGT GATGATAGAC TACTAATAGA AAAATTTATC 
GATAACCCTC GTCATATAGA AATCCAGGTT TTAGGGGATA AACATGGCAA TGCTCTGTGG 
CTCAATGAGA GGGAGTGCTC GATCCAGAGA AGGAACCAGA AGGTGGTAGA GGAGGCGCCA 
AGCATTTTTC TGGATCCTGA AACTCGCCAA GCAATGGGAG AGCAGGCCGT GGCTTTGGCT 
AAAGCCGTGA AGTATTCTTC TGCTGGAACT GTGGAATTTC TTGTGGACTC CCAGAAGAAT 
TTTTACTTCT TGGAGATGAA TACAAGACTA CAGGTCGAAC ATCCTGTCAC AGAGTGCATT 
ACTGGCCTGG ACTTAGTCCA AGAAATGATC CTTGTTGCTA AGGGTTACCC ACTCAGGCAC 
AAGCAAGAGG ATATTCCCAT CAGTGGCTGG GCAGTTGAAT GTCGGGTTTA TGCTGAGGAC 
CCCTACAAGT CTTTCGGTTT ACCGTCTATT GGGAGGCTGT CCCAGTACCA AGAGCCGATA 
CATCTACCTG GTGTCCGAGT TGACAGTGGC ATCCAACCAG GAAGTGACAT CAGCATCTAT 
TATGATCCTA TGATTTCAAA GCTAGTCACA TATGGGTCTG ACAGAGCAGA AGCCCTGAAG 
AGGATGGAAG ACGCACTGGA CAATTATGTG ATCCGGGGTG TTACACACAA CATCCCATTG 
CTCCGGGAGG TGATAATCAA CACACGTTTT GTGAAAGGAG ACATCAGCAC TAAGTTTCTC 
TCTGATGTGT ATCCTGATGG CTTCAAAGGG CACACGTTAA CACTGAGTGA GAGAAACCAG 
TTATTGGCCA TTGCATCATC TGTATTTGTG GCATCCCAGC TACGAGCTCA GCGCTTCCAA 
GAACATTCAA GAGTACCAGT TATTAGGCCT GATGTGGCTA AGTGGGAGCT CTCGGTAAAG 
TTACATGATG AAGATCATAC TGTCGTGGCA TCTAACAATG GGCCGGCATT TACCGTGGAA 
GTTGATGGCT CGAAACTAAA TGTGACCAGT ACGTGGAACC TGGCGTCACC CTTATTGTCT 
GTCAACGTTG ATGGCACGCA GAGGACTGTG CAGTACAAAG TGCACATTTT AACCAAGCTT 
GCTGCAGAGC TGAACAAATT CATGCTTGAA AAAGTGCCCA AGGACACCAG CAGCACTCTG 
TGCTCCCCGA TGCCTGGAGT GGTGGTGGCC GTTTCTGTCA AGCCTGGAGA CATGGTAGCA 
GAAGGTCAGG AAATCTGTGT GATTGAAGCT ATGAAAATGC AGAACAGTAT GACAGCTGGG 
AAAATGGGCA AGGTGAAATT GGTGCACTGC AAAGCTGGAG ACACAGTTGG TGAAGGAGAC 
CTGCTTGTGG AGCTGGAATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006518493.1
CDS	8055..10175
Translation	MAGQWVRTVALLAARRHWRRSSQQQLLGTLKHAPVYSYQCLVVSRSLSSVEYEPKEKTFDKILIANRGEIACRVIKTCKKMGIKTVAIHSDVDASSVHVKMADEAVCVGPAPTSKSYLNMDAIMEAIKKTRAQAVHPGYGFLSENKEFAKRLAAEDVTFIGPDTHAIQAMGDKIESKLLAKRAKVNTIPGFDGVVKDADEAVRIAREIGYPVMIKASAGGGGKGMRIAWDDEETRDGFRFSSQEAASSFGDDRLLIEKFIDNPRHIEIQVLGDKHGNALWLNERECSIQRRNQKVVEEAPSIFLDPETRQAMGEQAVALAKAVKYSSAGTVEFLVDSQKNFYFLEMNTRLQVEHPVTECITGLDLVQEMILVAKGYPLRHKQEDIPISGWAVECRVYAEDPYKSFGLPSIGRLSQYQEPIHLPGVRVDSGIQPGSDISIYYDPMISKLVTYGSDRAEALKRMEDALDNYVIRGVTHNIPLLREVIINTRFVKGDISTKFLSDVYPDGFKGHTLTLSERNQLLAIASSVFVASQLRAQRFQEHSRVPVIRPDVAKWELSVKLHDEDHTVVASNNGPAFTVEVDGSKLNVTSTWNLASPLLSVNVDGTQRTVQYKVHILTKLAAELNKFMLEKVPKDTSSTLCSPMPGVVVAVSVKPGDMVAEGQEICVIEAMKMQNSMTAGKMGKVKLVHCKAGDTVGEGDLLVELE

Target ORF information:

RefSeq Version	XM_006518430.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus propionyl-Coenzyme A carboxylase, alpha polypeptide (Pcca), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006518430.3

ORF Insert Sequence:

ATGGCGGGGC AGTGGGTCAG GACCGTGGCG CTGTTGGCGG CCAGGCGGCA TTGGCGGCGG 
TCCTCGCAGC AGCAATTGCT GGGGACGCTG AAGCATGCTC CAGTCTATTC ATACCAATGC 
CTAGTGGTGT CCAGAAGTCT CAGTTCTGTG GAATATGAGC CTAAAGAAAA GACTTTTGAT 
AAAATTCTCA TTGCTAACAG AGGAGAAATT GCCTGTAGGG TTATTAAAAC TTGCAAGAAG 
ATGGGCATCA AGACAGTTGC CATTCACAGT GATGTTGATG CCAGTTCTGT TCACGTGAAA 
ATGGCGGATG AGGCTGTCTG TGTTGGCCCA GCTCCCACCA GTAAAAGCTA CCTCAACATG 
GATGCCATCA TGGAAGCCAT TAAGAAAACC AGGGCCCAAG CTGTACACCC AGGGTATGGA 
TTCCTGTCAG AAAACAAAGA GTTTGCAAAG CGTCTGGCAG CAGAAGATGT CACTTTCATT 
GGACCTGATA CTCATGCTAT TCAAGCCATG GGTGACAAGA TAGAAAGCAA ACTATTAGCC 
AAGAGAGCAA AGGTCAACAC AATCCCTGGT TTTGATGGGG TAGTAAAGGA TGCAGATGAA 
GCTGTCAGAA TTGCAAGGGA AATTGGCTAC CCTGTGATGA TCAAGGCCTC AGCAGGCGGT 
GGTGGGAAAG GCATGCGCAT CGCCTGGGAT GACGAAGAGA CCAGGGATGG CTTTAGATTT 
TCATCCCAGG AAGCTGCTTC TAGTTTTGGT GATGATAGAC TACTAATAGA AAAATTTATC 
GATAACCCTC GTCATATAGA AATCCAGGTT TTAGGGGATA AACATGGCAA TGCTCTGTGG 
CTCAATGAGA GGGAGTGCTC GATCCAGAGA AGGAACCAGA AGGTGGTAGA GGAGGCGCCA 
AGCATTTTTC TGGATCCTGA AACTCGCCAA GCAATGGGAG AGCAGGCCGT GGCTTTGGCT 
AAAGCCGTGA AGTATTCTTC TGCTGGAACT GTGGAATTTC TTGTGGACTC CCAGAAGAAT 
TTTTACTTCT TGGAGATGAA TACAAGACTA CAGGTCGAAC ATCCTGTCAC AGAGTGCATT 
ACTGGCCTGG ACTTAGTCCA AGAAATGATC CTTGTTGCTA AGGGTTACCC ACTCAGGCAC 
AAGCAAGAGG ATATTCCCAT CAGTGGCTGG GCAGTTGAAT GTCGGGTTTA TGCTGAGGAC 
CCCTACAAGT CTTTCGGTTT ACCGTCTATT GGGAGGCTGT CCCAGTACCA AGAGCCGATA 
CATCTACCTG GTGTCCGAGT TGACAGTGGC ATCCAACCAG GAAGTGACAT CAGCATCTAT 
TATGATCCTA TGATTTCAAA GCTAGTCACA TATGGGTCTG ACAGAGCAGA AGCCCTGAAG 
AGGATGGAAG ACGCACTGGA CAATTATGTG ATCCGGGGTG TTACACACAA CATCCCATTG 
CTCCGGGAGG TGATAATCAA CACACGTTTT GTGAAAGGAG ACATCAGCAC TAAGTTTCTC 
TCTGATGTGT ATCCTGATGG CTTCAAAGGG CACACGTTAA CACTGAGTGA GAGAAACCAG 
TTATTGGCCA TTGCATCATC TGTATTTGTG GCATCCCAGC TACGAGCTCA GCGCTTCCAA 
GAACATTCAA GAGTACCAGT TATTAGGCCT GATGTGGCTA AGTGGGAGCT CTCGGTAAAG 
TTACATGATG AAGATCATAC TGTCGTGGCA TCTAACAATG GGCCGGCATT TACCGTGGAA 
GTTGATGGCT CGAAACTAAA TGTGACCAGT ACGTGGAACC TGGCGTCACC CTTATTGTCT 
GTCAACGTTG ATGGCACGCA GAGGACTGTG CAGTACAAAG TGCACATTTT AACCAAGCTT 
GCTGCAGAGC TGAACAAATT CATGCTTGAA AAAGTGCCCA AGGACACCAG CAGCACTCTG 
TGCTCCCCGA TGCCTGGAGT GGTGGTGGCC GTTTCTGTCA AGCCTGGAGA CATGGTAGCA 
GAAGGTCAGG AAATCTGTGT GATTGAAGCT ATGAAAATGC AGAACAGTAT GACAGCTGGG 
AAAATGGGCA AGGTGAAATT GGTGCACTGC AAAGCTGGAG ACACAGTTGG TGAAGGAGAC 
CTGCTTGTGG AGCTGGAATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Pcca ( NM_144844.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_659093.2 Mus musculus propionyl-Coenzyme A carboxylase, alpha polypeptide (Pcca), transcript variant 1, mRNA.

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CloneID	OMu15267
Clone ID Related Accession (Same CDS sequence)	NM_144844.2
Accession Version	NM_144844.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2175bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-15
Organism	Mus musculus(house mouse)
Product	propionyl-CoA carboxylase alpha chain, mitochondrial precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BY263345.1 and AK148002.1. On Jul 21, 2009 this sequence version replaced NM_144844.1. ##Evidence-Data-START## Transcript exon combination :: AK148002.1, AY046947.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGCGGGGC AGTGGGTCAG GACCGTGGCG CTGTTGGCGG CCAGGCGGCA TTGGCGGCGG 
TCCTCGCAGC AGCAATTGCT GGGGACGCTG AAGCATGCTC CAGTCTATTC ATACCAATGC 
CTAGTGGTGT CCAGAAGTCT CAGTTCTGTG GAATATGAGC CTAAAGAAAA GACTTTTGAT 
AAAATTCTCA TTGCTAACAG AGGAGAAATT GCCTGTAGGG TTATTAAAAC TTGCAAGAAG 
ATGGGCATCA AGACAGTTGC CATTCACAGT GATGTTGATG CCAGTTCTGT TCACGTGAAA 
ATGGCGGATG AGGCTGTCTG TGTTGGCCCA GCTCCCACCA GTAAAAGCTA CCTCAACATG 
GATGCCATCA TGGAAGCCAT TAAGAAAACC AGGGCCCAAG CTGTACACCC AGGGTATGGA 
TTCCTGTCAG AAAACAAAGA GTTTGCAAAG CGTCTGGCAG CAGAAGATGT CACTTTCATT 
GGACCTGATA CTCATGCTAT TCAAGCCATG GGTGACAAGA TAGAAAGCAA ACTATTAGCC 
AAGAGAGCAA AGGTCAACAC AATCCCTGGT TTTGATGGGG TAGTAAAGGA TGCAGATGAA 
GCTGTCAGAA TTGCAAGGGA AATTGGCTAC CCTGTGATGA TCAAGGCCTC AGCAGGCGGT 
GGTGGGAAAG GCATGCGCAT CGCCTGGGAT GACGAAGAGA CCAGGGATGG CTTTAGATTT 
TCATCCCAGG AAGCTGCTTC TAGTTTTGGT GATGATAGAC TACTAATAGA AAAATTTATC 
GATAACCCTC GTCATATAGA AATCCAGGTT TTAGGGGATA AACATGGCAA TGCTCTGTGG 
CTCAATGAGA GGGAGTGCTC GATCCAGAGA AGGAACCAGA AGGTGGTAGA GGAGGCGCCA 
AGCATTTTTC TGGATCCTGA AACTCGCCAA GCAATGGGAG AGCAGGCCGT GGCTTTGGCT 
AAAGCCGTGA AGTATTCTTC TGCTGGAACT GTGGAATTTC TTGTGGACTC CCAGAAGAAT 
TTTTACTTCT TGGAGATGAA TACAAGACTA CAGGTCGAAC ATCCTGTCAC AGAGTGCATT 
ACTGGCCTGG ACTTAGTCCA AGAAATGATC CTTGTTGCTA AGGGTTACCC ACTCAGGCAC 
AAGCAAGAGG ATATTCCCAT CAGTGGCTGG GCAGTTGAAT GTCGGGTTTA TGCTGAGGAC 
CCCTACAAGT CTTTCGGTTT ACCGTCTATT GGGAGGCTGT CCCAGTACCA AGAGCCGATA 
CATCTACCTG GTGTCCGAGT TGACAGTGGC ATCCAACCAG GAAGTGACAT CAGCATCTAT 
TATGATCCTA TGATTTCAAA GCTAGTCACA TATGGGTCTG ACAGAGCAGA AGCCCTGAAG 
AGGATGGAAG ACGCACTGGA CAATTATGTG ATCCGGGGTG TTACACACAA CATCCCATTG 
CTCCGGGAGG TGATAATCAA CACACGTTTT GTGAAAGGAG ACATCAGCAC TAAGTTTCTC 
TCTGATGTGT ATCCTGATGG CTTCAAAGGG CACACGTTAA CACTGAGTGA GAGAAACCAG 
TTATTGGCCA TTGCATCATC TGTATTTGTG GCATCCCAGC TACGAGCTCA GCGCTTCCAA 
GAACATTCAA GAGTACCAGT TATTAGGCCT GATGTGGCTA AGTGGGAGCT CTCGGTAAAG 
TTACATGATG AAGATCATAC TGTCGTGGCA TCTAACAATG GGCCGGCATT TACCGTGGAA 
GTTGATGGCT CGAAACTAAA TGTGACCAGT ACGTGGAACC TGGCGTCACC CTTATTGTCT 
GTCAACGTTG ATGGCACGCA GAGGACTGTG CAGTGTCTTT CTCGGGAAGC AGGTGGAAAC 
ATGAGCATCC AGTTTCTTGG CACAGTGTAC AAAGTGCACA TTTTAACCAA GCTTGCTGCA 
GAGCTGAACA AATTCATGCT TGAAAAAGTG CCCAAGGACA CCAGCAGCAC TCTGTGCTCC 
CCGATGCCTG GAGTGGTGGT GGCCGTTTCT GTCAAGCCTG GAGACATGGT AGCAGAAGGT 
CAGGAAATCT GTGTGATTGA AGCTATGAAA ATGCAGAACA GTATGACAGC TGGGAAAATG 
GGCAAGGTGA AATTGGTGCA CTGCAAAGCT GGAGACACAG TTGGTGAAGG AGACCTGCTT 
GTGGAGCTGG AATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_659093.2
CDS	40..2214
Translation	MAGQWVRTVALLAARRHWRRSSQQQLLGTLKHAPVYSYQCLVVSRSLSSVEYEPKEKTFDKILIANRGEIACRVIKTCKKMGIKTVAIHSDVDASSVHVKMADEAVCVGPAPTSKSYLNMDAIMEAIKKTRAQAVHPGYGFLSENKEFAKRLAAEDVTFIGPDTHAIQAMGDKIESKLLAKRAKVNTIPGFDGVVKDADEAVRIAREIGYPVMIKASAGGGGKGMRIAWDDEETRDGFRFSSQEAASSFGDDRLLIEKFIDNPRHIEIQVLGDKHGNALWLNERECSIQRRNQKVVEEAPSIFLDPETRQAMGEQAVALAKAVKYSSAGTVEFLVDSQKNFYFLEMNTRLQVEHPVTECITGLDLVQEMILVAKGYPLRHKQEDIPISGWAVECRVYAEDPYKSFGLPSIGRLSQYQEPIHLPGVRVDSGIQPGSDISIYYDPMISKLVTYGSDRAEALKRMEDALDNYVIRGVTHNIPLLREVIINTRFVKGDISTKFLSDVYPDGFKGHTLTLSERNQLLAIASSVFVASQLRAQRFQEHSRVPVIRPDVAKWELSVKLHDEDHTVVASNNGPAFTVEVDGSKLNVTSTWNLASPLLSVNVDGTQRTVQCLSREAGGNMSIQFLGTVYKVHILTKLAAELNKFMLEKVPKDTSSTLCSPMPGVVVAVSVKPGDMVAEGQEICVIEAMKMQNSMTAGKMGKVKLVHCKAGDTVGEGDLLVELE

Target ORF information:

RefSeq Version	NM_144844.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus propionyl-Coenzyme A carboxylase, alpha polypeptide (Pcca), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_144844.2

ORF Insert Sequence:

ATGGCGGGGC AGTGGGTCAG GACCGTGGCG CTGTTGGCGG CCAGGCGGCA TTGGCGGCGG 
TCCTCGCAGC AGCAATTGCT GGGGACGCTG AAGCATGCTC CAGTCTATTC ATACCAATGC 
CTAGTGGTGT CCAGAAGTCT CAGTTCTGTG GAATATGAGC CTAAAGAAAA GACTTTTGAT 
AAAATTCTCA TTGCTAACAG AGGAGAAATT GCCTGTAGGG TTATTAAAAC TTGCAAGAAG 
ATGGGCATCA AGACAGTTGC CATTCACAGT GATGTTGATG CCAGTTCTGT TCACGTGAAA 
ATGGCGGATG AGGCTGTCTG TGTTGGCCCA GCTCCCACCA GTAAAAGCTA CCTCAACATG 
GATGCCATCA TGGAAGCCAT TAAGAAAACC AGGGCCCAAG CTGTACACCC AGGGTATGGA 
TTCCTGTCAG AAAACAAAGA GTTTGCAAAG CGTCTGGCAG CAGAAGATGT CACTTTCATT 
GGACCTGATA CTCATGCTAT TCAAGCCATG GGTGACAAGA TAGAAAGCAA ACTATTAGCC 
AAGAGAGCAA AGGTCAACAC AATCCCTGGT TTTGATGGGG TAGTAAAGGA TGCAGATGAA 
GCTGTCAGAA TTGCAAGGGA AATTGGCTAC CCTGTGATGA TCAAGGCCTC AGCAGGCGGT 
GGTGGGAAAG GCATGCGCAT CGCCTGGGAT GACGAAGAGA CCAGGGATGG CTTTAGATTT 
TCATCCCAGG AAGCTGCTTC TAGTTTTGGT GATGATAGAC TACTAATAGA AAAATTTATC 
GATAACCCTC GTCATATAGA AATCCAGGTT TTAGGGGATA AACATGGCAA TGCTCTGTGG 
CTCAATGAGA GGGAGTGCTC GATCCAGAGA AGGAACCAGA AGGTGGTAGA GGAGGCGCCA 
AGCATTTTTC TGGATCCTGA AACTCGCCAA GCAATGGGAG AGCAGGCCGT GGCTTTGGCT 
AAAGCCGTGA AGTATTCTTC TGCTGGAACT GTGGAATTTC TTGTGGACTC CCAGAAGAAT 
TTTTACTTCT TGGAGATGAA TACAAGACTA CAGGTCGAAC ATCCTGTCAC AGAGTGCATT 
ACTGGCCTGG ACTTAGTCCA AGAAATGATC CTTGTTGCTA AGGGTTACCC ACTCAGGCAC 
AAGCAAGAGG ATATTCCCAT CAGTGGCTGG GCAGTTGAAT GTCGGGTTTA TGCTGAGGAC 
CCCTACAAGT CTTTCGGTTT ACCGTCTATT GGGAGGCTGT CCCAGTACCA AGAGCCGATA 
CATCTACCTG GTGTCCGAGT TGACAGTGGC ATCCAACCAG GAAGTGACAT CAGCATCTAT 
TATGATCCTA TGATTTCAAA GCTAGTCACA TATGGGTCTG ACAGAGCAGA AGCCCTGAAG 
AGGATGGAAG ACGCACTGGA CAATTATGTG ATCCGGGGTG TTACACACAA CATCCCATTG 
CTCCGGGAGG TGATAATCAA CACACGTTTT GTGAAAGGAG ACATCAGCAC TAAGTTTCTC 
TCTGATGTGT ATCCTGATGG CTTCAAAGGG CACACGTTAA CACTGAGTGA GAGAAACCAG 
TTATTGGCCA TTGCATCATC TGTATTTGTG GCATCCCAGC TACGAGCTCA GCGCTTCCAA 
GAACATTCAA GAGTACCAGT TATTAGGCCT GATGTGGCTA AGTGGGAGCT CTCGGTAAAG 
TTACATGATG AAGATCATAC TGTCGTGGCA TCTAACAATG GGCCGGCATT TACCGTGGAA 
GTTGATGGCT CGAAACTAAA TGTGACCAGT ACGTGGAACC TGGCGTCACC CTTATTGTCT 
GTCAACGTTG ATGGCACGCA GAGGACTGTG CAGTGTCTTT CTCGGGAAGC AGGTGGAAAC 
ATGAGCATCC AGTTTCTTGG CACAGTGTAC AAAGTGCACA TTTTAACCAA GCTTGCTGCA 
GAGCTGAACA AATTCATGCT TGAAAAAGTG CCCAAGGACA CCAGCAGCAC TCTGTGCTCC 
CCGATGCCTG GAGTGGTGGT GGCCGTTTCT GTCAAGCCTG GAGACATGGT AGCAGAAGGT 
CAGGAAATCT GTGTGATTGA AGCTATGAAA ATGCAGAACA GTATGACAGC TGGGAAAATG 
GGCAAGGTGA AATTGGTGCA CTGCAAAGCT GGAGACACAG TTGGTGAAGG AGACCTGCTT 
GTGGAGCTGG AATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Pcca ( XM_006518430.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006518493.1 Mus musculus propionyl-Coenzyme A carboxylase, alpha polypeptide (Pcca), transcript variant X1, mRNA.

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CloneID	OMu49587
Clone ID Related Accession (Same CDS sequence)	XM_006518430.4 , XM_006518430.3
Accession Version	XM_006518430.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2121bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	propionyl-CoA carboxylase alpha chain, mitochondrial isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000080.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006518430.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGGGC AGTGGGTCAG GACCGTGGCG CTGTTGGCGG CCAGGCGGCA TTGGCGGCGG 
TCCTCGCAGC AGCAATTGCT GGGGACGCTG AAGCATGCTC CAGTCTATTC ATACCAATGC 
CTAGTGGTGT CCAGAAGTCT CAGTTCTGTG GAATATGAGC CTAAAGAAAA GACTTTTGAT 
AAAATTCTCA TTGCTAACAG AGGAGAAATT GCCTGTAGGG TTATTAAAAC TTGCAAGAAG 
ATGGGCATCA AGACAGTTGC CATTCACAGT GATGTTGATG CCAGTTCTGT TCACGTGAAA 
ATGGCGGATG AGGCTGTCTG TGTTGGCCCA GCTCCCACCA GTAAAAGCTA CCTCAACATG 
GATGCCATCA TGGAAGCCAT TAAGAAAACC AGGGCCCAAG CTGTACACCC AGGGTATGGA 
TTCCTGTCAG AAAACAAAGA GTTTGCAAAG CGTCTGGCAG CAGAAGATGT CACTTTCATT 
GGACCTGATA CTCATGCTAT TCAAGCCATG GGTGACAAGA TAGAAAGCAA ACTATTAGCC 
AAGAGAGCAA AGGTCAACAC AATCCCTGGT TTTGATGGGG TAGTAAAGGA TGCAGATGAA 
GCTGTCAGAA TTGCAAGGGA AATTGGCTAC CCTGTGATGA TCAAGGCCTC AGCAGGCGGT 
GGTGGGAAAG GCATGCGCAT CGCCTGGGAT GACGAAGAGA CCAGGGATGG CTTTAGATTT 
TCATCCCAGG AAGCTGCTTC TAGTTTTGGT GATGATAGAC TACTAATAGA AAAATTTATC 
GATAACCCTC GTCATATAGA AATCCAGGTT TTAGGGGATA AACATGGCAA TGCTCTGTGG 
CTCAATGAGA GGGAGTGCTC GATCCAGAGA AGGAACCAGA AGGTGGTAGA GGAGGCGCCA 
AGCATTTTTC TGGATCCTGA AACTCGCCAA GCAATGGGAG AGCAGGCCGT GGCTTTGGCT 
AAAGCCGTGA AGTATTCTTC TGCTGGAACT GTGGAATTTC TTGTGGACTC CCAGAAGAAT 
TTTTACTTCT TGGAGATGAA TACAAGACTA CAGGTCGAAC ATCCTGTCAC AGAGTGCATT 
ACTGGCCTGG ACTTAGTCCA AGAAATGATC CTTGTTGCTA AGGGTTACCC ACTCAGGCAC 
AAGCAAGAGG ATATTCCCAT CAGTGGCTGG GCAGTTGAAT GTCGGGTTTA TGCTGAGGAC 
CCCTACAAGT CTTTCGGTTT ACCGTCTATT GGGAGGCTGT CCCAGTACCA AGAGCCGATA 
CATCTACCTG GTGTCCGAGT TGACAGTGGC ATCCAACCAG GAAGTGACAT CAGCATCTAT 
TATGATCCTA TGATTTCAAA GCTAGTCACA TATGGGTCTG ACAGAGCAGA AGCCCTGAAG 
AGGATGGAAG ACGCACTGGA CAATTATGTG ATCCGGGGTG TTACACACAA CATCCCATTG 
CTCCGGGAGG TGATAATCAA CACACGTTTT GTGAAAGGAG ACATCAGCAC TAAGTTTCTC 
TCTGATGTGT ATCCTGATGG CTTCAAAGGG CACACGTTAA CACTGAGTGA GAGAAACCAG 
TTATTGGCCA TTGCATCATC TGTATTTGTG GCATCCCAGC TACGAGCTCA GCGCTTCCAA 
GAACATTCAA GAGTACCAGT TATTAGGCCT GATGTGGCTA AGTGGGAGCT CTCGGTAAAG 
TTACATGATG AAGATCATAC TGTCGTGGCA TCTAACAATG GGCCGGCATT TACCGTGGAA 
GTTGATGGCT CGAAACTAAA TGTGACCAGT ACGTGGAACC TGGCGTCACC CTTATTGTCT 
GTCAACGTTG ATGGCACGCA GAGGACTGTG CAGTACAAAG TGCACATTTT AACCAAGCTT 
GCTGCAGAGC TGAACAAATT CATGCTTGAA AAAGTGCCCA AGGACACCAG CAGCACTCTG 
TGCTCCCCGA TGCCTGGAGT GGTGGTGGCC GTTTCTGTCA AGCCTGGAGA CATGGTAGCA 
GAAGGTCAGG AAATCTGTGT GATTGAAGCT ATGAAAATGC AGAACAGTAT GACAGCTGGG 
AAAATGGGCA AGGTGAAATT GGTGCACTGC AAAGCTGGAG ACACAGTTGG TGAAGGAGAC 
CTGCTTGTGG AGCTGGAATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006518493.1
CDS	145..2265
Translation	MAGQWVRTVALLAARRHWRRSSQQQLLGTLKHAPVYSYQCLVVSRSLSSVEYEPKEKTFDKILIANRGEIACRVIKTCKKMGIKTVAIHSDVDASSVHVKMADEAVCVGPAPTSKSYLNMDAIMEAIKKTRAQAVHPGYGFLSENKEFAKRLAAEDVTFIGPDTHAIQAMGDKIESKLLAKRAKVNTIPGFDGVVKDADEAVRIAREIGYPVMIKASAGGGGKGMRIAWDDEETRDGFRFSSQEAASSFGDDRLLIEKFIDNPRHIEIQVLGDKHGNALWLNERECSIQRRNQKVVEEAPSIFLDPETRQAMGEQAVALAKAVKYSSAGTVEFLVDSQKNFYFLEMNTRLQVEHPVTECITGLDLVQEMILVAKGYPLRHKQEDIPISGWAVECRVYAEDPYKSFGLPSIGRLSQYQEPIHLPGVRVDSGIQPGSDISIYYDPMISKLVTYGSDRAEALKRMEDALDNYVIRGVTHNIPLLREVIINTRFVKGDISTKFLSDVYPDGFKGHTLTLSERNQLLAIASSVFVASQLRAQRFQEHSRVPVIRPDVAKWELSVKLHDEDHTVVASNNGPAFTVEVDGSKLNVTSTWNLASPLLSVNVDGTQRTVQYKVHILTKLAAELNKFMLEKVPKDTSSTLCSPMPGVVVAVSVKPGDMVAEGQEICVIEAMKMQNSMTAGKMGKVKLVHCKAGDTVGEGDLLVELE

Target ORF information:

RefSeq Version	XM_006518430.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus propionyl-Coenzyme A carboxylase, alpha polypeptide (Pcca), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006518430.4

ORF Insert Sequence:

ATGGCGGGGC AGTGGGTCAG GACCGTGGCG CTGTTGGCGG CCAGGCGGCA TTGGCGGCGG 
TCCTCGCAGC AGCAATTGCT GGGGACGCTG AAGCATGCTC CAGTCTATTC ATACCAATGC 
CTAGTGGTGT CCAGAAGTCT CAGTTCTGTG GAATATGAGC CTAAAGAAAA GACTTTTGAT 
AAAATTCTCA TTGCTAACAG AGGAGAAATT GCCTGTAGGG TTATTAAAAC TTGCAAGAAG 
ATGGGCATCA AGACAGTTGC CATTCACAGT GATGTTGATG CCAGTTCTGT TCACGTGAAA 
ATGGCGGATG AGGCTGTCTG TGTTGGCCCA GCTCCCACCA GTAAAAGCTA CCTCAACATG 
GATGCCATCA TGGAAGCCAT TAAGAAAACC AGGGCCCAAG CTGTACACCC AGGGTATGGA 
TTCCTGTCAG AAAACAAAGA GTTTGCAAAG CGTCTGGCAG CAGAAGATGT CACTTTCATT 
GGACCTGATA CTCATGCTAT TCAAGCCATG GGTGACAAGA TAGAAAGCAA ACTATTAGCC 
AAGAGAGCAA AGGTCAACAC AATCCCTGGT TTTGATGGGG TAGTAAAGGA TGCAGATGAA 
GCTGTCAGAA TTGCAAGGGA AATTGGCTAC CCTGTGATGA TCAAGGCCTC AGCAGGCGGT 
GGTGGGAAAG GCATGCGCAT CGCCTGGGAT GACGAAGAGA CCAGGGATGG CTTTAGATTT 
TCATCCCAGG AAGCTGCTTC TAGTTTTGGT GATGATAGAC TACTAATAGA AAAATTTATC 
GATAACCCTC GTCATATAGA AATCCAGGTT TTAGGGGATA AACATGGCAA TGCTCTGTGG 
CTCAATGAGA GGGAGTGCTC GATCCAGAGA AGGAACCAGA AGGTGGTAGA GGAGGCGCCA 
AGCATTTTTC TGGATCCTGA AACTCGCCAA GCAATGGGAG AGCAGGCCGT GGCTTTGGCT 
AAAGCCGTGA AGTATTCTTC TGCTGGAACT GTGGAATTTC TTGTGGACTC CCAGAAGAAT 
TTTTACTTCT TGGAGATGAA TACAAGACTA CAGGTCGAAC ATCCTGTCAC AGAGTGCATT 
ACTGGCCTGG ACTTAGTCCA AGAAATGATC CTTGTTGCTA AGGGTTACCC ACTCAGGCAC 
AAGCAAGAGG ATATTCCCAT CAGTGGCTGG GCAGTTGAAT GTCGGGTTTA TGCTGAGGAC 
CCCTACAAGT CTTTCGGTTT ACCGTCTATT GGGAGGCTGT CCCAGTACCA AGAGCCGATA 
CATCTACCTG GTGTCCGAGT TGACAGTGGC ATCCAACCAG GAAGTGACAT CAGCATCTAT 
TATGATCCTA TGATTTCAAA GCTAGTCACA TATGGGTCTG ACAGAGCAGA AGCCCTGAAG 
AGGATGGAAG ACGCACTGGA CAATTATGTG ATCCGGGGTG TTACACACAA CATCCCATTG 
CTCCGGGAGG TGATAATCAA CACACGTTTT GTGAAAGGAG ACATCAGCAC TAAGTTTCTC 
TCTGATGTGT ATCCTGATGG CTTCAAAGGG CACACGTTAA CACTGAGTGA GAGAAACCAG 
TTATTGGCCA TTGCATCATC TGTATTTGTG GCATCCCAGC TACGAGCTCA GCGCTTCCAA 
GAACATTCAA GAGTACCAGT TATTAGGCCT GATGTGGCTA AGTGGGAGCT CTCGGTAAAG 
TTACATGATG AAGATCATAC TGTCGTGGCA TCTAACAATG GGCCGGCATT TACCGTGGAA 
GTTGATGGCT CGAAACTAAA TGTGACCAGT ACGTGGAACC TGGCGTCACC CTTATTGTCT 
GTCAACGTTG ATGGCACGCA GAGGACTGTG CAGTACAAAG TGCACATTTT AACCAAGCTT 
GCTGCAGAGC TGAACAAATT CATGCTTGAA AAAGTGCCCA AGGACACCAG CAGCACTCTG 
TGCTCCCCGA TGCCTGGAGT GGTGGTGGCC GTTTCTGTCA AGCCTGGAGA CATGGTAGCA 
GAAGGTCAGG AAATCTGTGT GATTGAAGCT ATGAAAATGC AGAACAGTAT GACAGCTGGG 
AAAATGGGCA AGGTGAAATT GGTGCACTGC AAAGCTGGAG ACACAGTTGG TGAAGGAGAC 
CTGCTTGTGG AGCTGGAATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Pcca ( XM_030247609.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030103469.1 Mus musculus propionyl-Coenzyme A carboxylase, alpha polypeptide (Pcca), transcript variant X4, mRNA.

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CloneID	OMu49590
Clone ID Related Accession (Same CDS sequence)	XM_030247609.1
Accession Version	XM_030247609.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1635bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	propionyl-CoA carboxylase alpha chain, mitochondrial isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000080.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGGGC AGTGGGTCAG GACCGTGGCG CTGTTGGCGG CCAGGCGGCA TTGGCGGCGG 
TCCTCGCAGC AGCAATTGCT GGGGACGCTG AAGCATGCTC CAGTCTATTC ATACCAATGC 
CTAGTGGTGT CCAGAAGTCT CAGTTCTGTG GAATATGAGC CTAAAGAAAA GACTTTTGAT 
AAAATTCTCA TTGCTAACAG AGGAGAAATT GCCTGTAGGG TTATTAAAAC TTGCAAGAAG 
ATGGGCATCA AGACAGTTGC CATTCACAGT GATGTTGATG CCAGTTCTGT TCACGTGAAA 
ATGGCGGATG AGGCTGTCTG TGTTGGCCCA GCTCCCACCA GTAAAAGCTA CCTCAACATG 
GATGCCATCA TGGAAGCCAT TAAGAAAACC AGGGCCCAAG CTGTACACCC AGGGTATGGA 
TTCCTGTCAG AAAACAAAGA GTTTGCAAAG CGTCTGGCAG CAGAAGATGT CACTTTCATT 
GGACCTGATA CTCATGCTAT TCAAGCCATG GGTGACAAGA TAGAAAGCAA ACTATTAGCC 
AAGAGAGCAA AGGTCAACAC AATCCCTGGT TTTGATGGGG TAGTAAAGGA TGCAGATGAA 
GCTGTCAGAA TTGCAAGGGA AATTGGCTAC CCTGTGATGA TCAAGGCCTC AGCAGGCGGT 
GGTGGGAAAG GCATGCGCAT CGCCTGGGAT GACGAAGAGA CCAGGGATGG CTTTAGATTT 
TCATCCCAGG AAGCTGCTTC TAGTTTTGGT GATGATAGAC TACTAATAGA AAAATTTATC 
GATAACCCTC GTCATATAGA AATCCAGGTT TTAGGGGATA AACATGGCAA TGCTCTGTGG 
CTCAATGAGA GGGAGTGCTC GATCCAGAGA AGGAACCAGA AGGTGGTAGA GGAGGCGCCA 
AGCATTTTTC TGGATCCTGA AACTCGCCAA GCAATGGGAG AGCAGGCCGT GGCTTTGGCT 
AAAGCCGTGA AGTATTCTTC TGCTGGAACT GTGGAATTTC TTGTGGACTC CCAGAAGAAT 
TTTTACTTCT TGGAGATGAA TACAAGACTA CAGGTCGAAC ATCCTGTCAC AGAGTGCATT 
ACTGGCCTGG ACTTAGTCCA AGAAATGATC CTTGTTGCTA AGGGTTACCC ACTCAGGCAC 
AAGCAAGAGG ATATTCCCAT CAGTGGCTGG GCAGTTGAAT GTCGGGTTTA TGCTGAGGAC 
CCCTACAAGT CTTTCGGTTT ACCGTCTATT GGGAGGCTGT CCCAGTACCA AGAGCCGATA 
CATCTACCTG GTGTCCGAGT TGACAGTGGC ATCCAACCAG GAAGTGACAT CAGCATCTAT 
TATGATCCTA TGATTTCAAA GCTAGTCACA TATGGGTCTG ACAGAGCAGA AGCCCTGAAG 
AGGATGGAAG ACGCACTGGA CAATTATGTG ATCCGGGGTG TTACACACAA CATCCCATTG 
CTCCGGGAGG TGATAATCAA CACACGTTTT GTGAAAGGAG ACATCAGCAC TAAGTTTCTC 
TCTGATGTGT ATCCTGATGG CTTCAAAGGG CACACGTTAA CACTGAGTGA GAGAAACCAG 
TTATTGGCCA TTGCATCATC TGTATTTGTG GCATCCCAGC TACGAGCTCA GCGCTTCCAA 
GAACATTCAA GATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030103469.1
CDS	8055..9689
Translation	MAGQWVRTVALLAARRHWRRSSQQQLLGTLKHAPVYSYQCLVVSRSLSSVEYEPKEKTFDKILIANRGEIACRVIKTCKKMGIKTVAIHSDVDASSVHVKMADEAVCVGPAPTSKSYLNMDAIMEAIKKTRAQAVHPGYGFLSENKEFAKRLAAEDVTFIGPDTHAIQAMGDKIESKLLAKRAKVNTIPGFDGVVKDADEAVRIAREIGYPVMIKASAGGGGKGMRIAWDDEETRDGFRFSSQEAASSFGDDRLLIEKFIDNPRHIEIQVLGDKHGNALWLNERECSIQRRNQKVVEEAPSIFLDPETRQAMGEQAVALAKAVKYSSAGTVEFLVDSQKNFYFLEMNTRLQVEHPVTECITGLDLVQEMILVAKGYPLRHKQEDIPISGWAVECRVYAEDPYKSFGLPSIGRLSQYQEPIHLPGVRVDSGIQPGSDISIYYDPMISKLVTYGSDRAEALKRMEDALDNYVIRGVTHNIPLLREVIINTRFVKGDISTKFLSDVYPDGFKGHTLTLSERNQLLAIASSVFVASQLRAQRFQEHSR

Target ORF information:

RefSeq Version	XM_030247609.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus propionyl-Coenzyme A carboxylase, alpha polypeptide (Pcca), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030247609.1

ORF Insert Sequence:

ATGGCGGGGC AGTGGGTCAG GACCGTGGCG CTGTTGGCGG CCAGGCGGCA TTGGCGGCGG 
TCCTCGCAGC AGCAATTGCT GGGGACGCTG AAGCATGCTC CAGTCTATTC ATACCAATGC 
CTAGTGGTGT CCAGAAGTCT CAGTTCTGTG GAATATGAGC CTAAAGAAAA GACTTTTGAT 
AAAATTCTCA TTGCTAACAG AGGAGAAATT GCCTGTAGGG TTATTAAAAC TTGCAAGAAG 
ATGGGCATCA AGACAGTTGC CATTCACAGT GATGTTGATG CCAGTTCTGT TCACGTGAAA 
ATGGCGGATG AGGCTGTCTG TGTTGGCCCA GCTCCCACCA GTAAAAGCTA CCTCAACATG 
GATGCCATCA TGGAAGCCAT TAAGAAAACC AGGGCCCAAG CTGTACACCC AGGGTATGGA 
TTCCTGTCAG AAAACAAAGA GTTTGCAAAG CGTCTGGCAG CAGAAGATGT CACTTTCATT 
GGACCTGATA CTCATGCTAT TCAAGCCATG GGTGACAAGA TAGAAAGCAA ACTATTAGCC 
AAGAGAGCAA AGGTCAACAC AATCCCTGGT TTTGATGGGG TAGTAAAGGA TGCAGATGAA 
GCTGTCAGAA TTGCAAGGGA AATTGGCTAC CCTGTGATGA TCAAGGCCTC AGCAGGCGGT 
GGTGGGAAAG GCATGCGCAT CGCCTGGGAT GACGAAGAGA CCAGGGATGG CTTTAGATTT 
TCATCCCAGG AAGCTGCTTC TAGTTTTGGT GATGATAGAC TACTAATAGA AAAATTTATC 
GATAACCCTC GTCATATAGA AATCCAGGTT TTAGGGGATA AACATGGCAA TGCTCTGTGG 
CTCAATGAGA GGGAGTGCTC GATCCAGAGA AGGAACCAGA AGGTGGTAGA GGAGGCGCCA 
AGCATTTTTC TGGATCCTGA AACTCGCCAA GCAATGGGAG AGCAGGCCGT GGCTTTGGCT 
AAAGCCGTGA AGTATTCTTC TGCTGGAACT GTGGAATTTC TTGTGGACTC CCAGAAGAAT 
TTTTACTTCT TGGAGATGAA TACAAGACTA CAGGTCGAAC ATCCTGTCAC AGAGTGCATT 
ACTGGCCTGG ACTTAGTCCA AGAAATGATC CTTGTTGCTA AGGGTTACCC ACTCAGGCAC 
AAGCAAGAGG ATATTCCCAT CAGTGGCTGG GCAGTTGAAT GTCGGGTTTA TGCTGAGGAC 
CCCTACAAGT CTTTCGGTTT ACCGTCTATT GGGAGGCTGT CCCAGTACCA AGAGCCGATA 
CATCTACCTG GTGTCCGAGT TGACAGTGGC ATCCAACCAG GAAGTGACAT CAGCATCTAT 
TATGATCCTA TGATTTCAAA GCTAGTCACA TATGGGTCTG ACAGAGCAGA AGCCCTGAAG 
AGGATGGAAG ACGCACTGGA CAATTATGTG ATCCGGGGTG TTACACACAA CATCCCATTG 
CTCCGGGAGG TGATAATCAA CACACGTTTT GTGAAAGGAG ACATCAGCAC TAAGTTTCTC 
TCTGATGTGT ATCCTGATGG CTTCAAAGGG CACACGTTAA CACTGAGTGA GAGAAACCAG 
TTATTGGCCA TTGCATCATC TGTATTTGTG GCATCCCAGC TACGAGCTCA GCGCTTCCAA 
GAACATTCAA GATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Dysregulated miRNAs and their pathogenic implications for the neurometabolic disease propionic acidemia.
Scientific reports7(1)5727(2017 Jul)
Rivera-Barahona A,Fulgencio-Covi?n A,P?rez-Cerd? C,Ramos R,Barry MA,Ugarte M,P?rez B,Richard E,Desviat LR

CLUH regulates mitochondrial metabolism by controlling translation and decay of target mRNAs.
The Journal of cell biology216(3)675-693(2017 Mar)
Schatton D,Pla-Martin D,Marx MC,Hansen H,Mourier A,Nemazanyy I,Pessia A,Zentis P,Corona T,Kondylis V,Barth E,Schauss AC,Velagapudi V,Rugarli EI

In vivo evidence of mitochondrial dysfunction and altered redox homeostasis in a genetic mouse model of propionic acidemia: Implications for the pathophysiology of this disorder.
Free radical biology & medicine961-12(2016 07)
Gallego-Villar L,Rivera-Barahona A,Cuevas-Mart?n C,Guenzel A,P?rez B,Barry MA,Murphy MP,Logan A,Gonzalez-Quintana A,Mart?n MA,Medina S,Gil-Izquierdo A,Cuezva JM,Richard E,Desviat LR

Long-term sex-biased correction of circulating propionic acidemia disease markers by adeno-associated virus vectors.
Human gene therapy26(3)153-60(2015 Mar)
Guenzel AJ,Collard R,Kraus JP,Matern D,Barry MA

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Pcca cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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