TBX10 cDNA ORF clone, Mus musculus(House mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	TBX10
Entrez Gene ID	109575
Full Name	T-box 10
Synonyms	Dc,Tbx13,Tbx7
General protein information	Preferred Names T-box 10 Names T-box transcription factor TBX10 T-box 13 T-box protein 10 T-box protein 13 dancer mmTBX7
Gene Type	protein-coding
Organism	Mus musculus(House mouse)
Genome	Chromosome: 19 Map Location: 19 A\|19 3.71 cM

mRNA and Protein(s)

mRNA	Protein	Name
XM_006531631.3	XP_006531694.1	T-box transcription factor TBX10 isoform X1
NM_011533.2	NP_035663.1	T-box transcription factor TBX10 isoform 2
NM_001001320.1	NP_001001320.1	T-box transcription factor TBX10 isoform 1
XM_006531632.2	XP_006531695.1	T-box transcription factor TBX10 isoform X1

Pathways from BioSystems

Homologies

	LOC721689	XP_001117884.2
Canis lupus familiaris (dog)	TBX10	XP_005631453.1
Bos taurus (cattle)	TBX10	XP_002699446.2
Mus musculus (house mouse)	Tbx10	NP_001001320.1
Homo sapiens (human)	TBX10	NP_005986.2
Pan troglodytes (chimpanzee)	TBX10	XP_001152773.1
Rattus norvegicus (Norway rat)	Tbx10	XP_006230983.1
Gallus gallus (chicken)	TBX10	XP_426372.3

Gene Ontology
Bibliography

Related articles in PubMed

High-throughput discovery of novel developmental phenotypes.
Dickinson ME, Flenniken AM, Ji X, Teboul L, Wong MD, White JK, Meehan TF, Weninger WJ, Westerberg H, Adissu H, Baker CN, Bower L, Brown JM, Caddle LB, Chiani F, Clary D, Cleak J, Daly MJ, Denegre JM, Doe B, Dolan ME, Edie SM, Fuchs H, Gailus-Durner V, Galli A, Gambadoro A, Gallegos J, Guo S, Horner NR, Hsu CW, Johnson SJ, Kalaga S, Keith LC, Lanoue L, Lawson TN, Lek M, Mark M, Marschall S, Mason J, McElwee ML, Newbigging S, Nutter LM, Peterson KA, Ramirez-Solis R, Rowland DJ, Ryder E, Samocha KE, Seavitt JR, Selloum M, Szoke-Kovacs Z, Tamura M, Trainor AG, Tudose I, Wakana S, Warren J, Wendling O, West DB, Wong L, Yoshiki A, , , , , , , , , MacArthur DG, Tocchini-Valentini GP, Gao X, Flicek P, Bradley A, Skarnes WC, Justice MJ, Parkinson HE, Moore M, Wells S, Braun RE, Svenson KL, de Angelis MH, Herault Y, Mohun T, Mallon AM, Henkelman RM, Brown SD, Adams DJ, Lloyd KC, McKerlie C, Beaudet AL, Bu?an M, Murray SA
Nature537(7621)508-514(2016 09)

The International Mouse Phenotyping Consortium Web Portal, a unified point of access for knockout mice and related phenotyping data.
Koscielny G, Yaikhom G, Iyer V, Meehan TF, Morgan H, Atienza-Herrero J, Blake A, Chen CK, Easty R, Di Fenza A, Fiegel T, Grifiths M, Horne A, Karp NA, Kurbatova N, Mason JC, Matthews P, Oakley DJ, Qazi A, Regnart J, Retha A, Santos LA, Sneddon DJ, Warren J, Westerberg H, Wilson RJ, Melvin DG, Smedley D, Brown SD, Flicek P, Skarnes WC, Mallon AM, Parkinson H
Nucleic acids research42(Database issue)D802-9(2014 Jan)

SOX2 regulates the hypothalamic-pituitary axis at multiple levels.
Jayakody SA, Andoniadou CL, Gaston-Massuet C, Signore M, Cariboni A, Bouloux PM, Le Tissier P, Pevny LH, Dattani MT, Martinez-Barbera JP
The Journal of clinical investigation122(10)3635-46(2012 Oct)

A unique expression pattern of Tbx10 in the hindbrain as revealed by Tbx10(LacZ) allele.
Xue XD, Kimura W, Wang B, Hikosaka K, Itakura T, Uezato T, Matsuda M, Koseki H, Miura N
Genesis (New York, N.Y. : 2000)48(5)295-302(2010 May)

A systems approach reveals that the myogenesis genome network is regulated by the transcriptional repressor RP58.
Yokoyama S, Ito Y, Ueno-Kudoh H, Shimizu H, Uchibe K, Albini S, Mitsuoka K, Miyaki S, Kiso M, Nagai A, Hikata T, Osada T, Fukuda N, Yamashita S, Harada D, Mezzano V, Kasai M, Puri PL, Hayashizaki Y, Okado H, Hashimoto M, Asahara H
Developmental cell17(6)836-48(2009 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Data show that the LacZ-marked Tbx10 expressing cells are likely to be a subpopulation of interneurons in the ventral pons.
Title: A unique expression pattern of Tbx10 in the hindbrain as revealed by Tbx10(LacZ) allele.

The following TBX10 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the TBX10 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu54998	XM_006531631.3 Latest version!	Mus musculus T-box 10 (Tbx10), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
OMu09480	NM_001001320.1 Latest version!	Mus musculus T-box 10 (Tbx10), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$188.30 $269.00
OMu54999	NM_011533.2 Latest version!	Mus musculus T-box 10 (Tbx10), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$223.30 $319.00
OMu54999	XM_006531632.2 Latest version!	Mus musculus T-box 10 (Tbx10), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$223.30 $319.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

TBX10 ( XM_006531631.3 ) cDNA ORF clone, Mus musculus(House mouse) -> XP_006531694.1 Mus musculus T-box 10 (Tbx10), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu54998
Clone ID Related Accession (Same CDS sequence)	XM_006531631.3
Accession Version	XM_006531631.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1128bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	T-box transcription factor TBX10 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000085.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006531631.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGTCT TCCTGTCTGC TGGCCTTGGG GTACTTGCTC CTCCAGAGAC CTGTCCCCAT 
CCCGAGACCA GCACCAGCTG GGAGTCCCGG CTGGGGACAC GATTCCCCTC AGGTTCCTGC 
ACCATGTCTA CAGAAGCCCA AGCCATGGCA GAGCCCACTG GGCAGGGCCC CAAGAACCCA 
CGTGTGTCCA GTGTGATGGT GCAGCTGGAG ATGAAGCCAC TGTGGGAAGA GTTCAACCAG 
CTGGGCACAG AGATGATTGT CACCAAAGCG GGCAGGAGGA TGTTCCCAAC GTTCCAGGTG 
AAGATCCTGG GCATGGACAC GCTGGCTGAC TACGCTTTGC TCATGGACTT TATCCCGTTG 
GATGACAAGA GATACAGGTA CGCATTCCAC AGTTCTGCCT GGCTGGTGGC AGGCAAGGCA 
GACCCTGCCA CGCCTGGCAG GGTGCACTTC CACCCTGACT CGCCAGCCAA GGGTGCCCAG 
TGGATGCGGC AGATTGTGTC CTTTGACAAA CTCAAGCTGA CCAACAACCT GATGGACGAC 
AATGGCCACC CCCGTTTCCA CGTGGTCTTC GTGGACCCAC GCAAGGACAG TGCCCGCTAT 
GCCCAGGAAA ACTTCAAGTC CTTTGTCTTC ACGGAGACCC AGTTTACAGC TGTGACAGCC 
TATCAGAACC ACAGGATCAC GCAGCTGAAA ATTGCTAGCA ACCCCTTTGC CAAAGGTTTC 
AGAGAGGCTG ACCCAGACTC ATGGCCTGTG ACCCCCCGGC CACTGCTCAG CATCCCTGCC 
CGGAGTCGTA GCAGCCTCAG TCCCTGCCTC CTTAAGGGCT CTGCAGAACG AGAGAAAGAT 
ACCAGTAAAG CTTCCGCTTC CAGCTCCAGG ACCCCTACTC AGCCACACAA TCAGCTGCTG 
CCTGCCCCTG ATGTCCTGCT AGCCCCTGCC ACCTACAGAC CCCTCCCTTA CCAGAACCTG 
TACCCTGGAT CCCCAAGCCG TGCTGGGCCC CCAAGAGCTC GGCTGGCACC TTACCCTCTC 
CCCAACATCA GCACGGCTGG AGATCAGGAG GACCCAACCC TCGCAGCTGG GCTGGGACTC 
CTGCCCACCT CTGCCTTGTG CTTGGTGTCT AATCAAGCCT CGCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006531694.1
CDS	317..1444
Translation	MAVFLSAGLGVLAPPETCPHPETSTSWESRLGTRFPSGSCTMSTEAQAMAEPTGQGPKNPRVSSVMVQLEMKPLWEEFNQLGTEMIVTKAGRRMFPTFQVKILGMDTLADYALLMDFIPLDDKRYRYAFHSSAWLVAGKADPATPGRVHFHPDSPAKGAQWMRQIVSFDKLKLTNNLMDDNGHPRFHVVFVDPRKDSARYAQENFKSFVFTETQFTAVTAYQNHRITQLKIASNPFAKGFREADPDSWPVTPRPLLSIPARSRSSLSPCLLKGSAEREKDTSKASASSSRTPTQPHNQLLPAPDVLLAPATYRPLPYQNLYPGSPSRAGPPRARLAPYPLPNISTAGDQEDPTLAAGLGLLPTSALCLVSNQASQ

Target ORF information:

RefSeq Version	XM_006531631.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus T-box 10 (Tbx10), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006531631.3

ORF Insert Sequence:

ATGGCAGTCT TCCTGTCTGC TGGCCTTGGG GTACTTGCTC CTCCAGAGAC CTGTCCCCAT 
CCCGAGACCA GCACCAGCTG GGAGTCCCGG CTGGGGACAC GATTCCCCTC AGGTTCCTGC 
ACCATGTCTA CAGAAGCCCA AGCCATGGCA GAGCCCACTG GGCAGGGCCC CAAGAACCCA 
CGTGTGTCCA GTGTGATGGT GCAGCTGGAG ATGAAGCCAC TGTGGGAAGA GTTCAACCAG 
CTGGGCACAG AGATGATTGT CACCAAAGCG GGCAGGAGGA TGTTCCCAAC GTTCCAGGTG 
AAGATCCTGG GCATGGACAC GCTGGCTGAC TACGCTTTGC TCATGGACTT TATCCCGTTG 
GATGACAAGA GATACAGGTA CGCATTCCAC AGTTCTGCCT GGCTGGTGGC AGGCAAGGCA 
GACCCTGCCA CGCCTGGCAG GGTGCACTTC CACCCTGACT CGCCAGCCAA GGGTGCCCAG 
TGGATGCGGC AGATTGTGTC CTTTGACAAA CTCAAGCTGA CCAACAACCT GATGGACGAC 
AATGGCCACC CCCGTTTCCA CGTGGTCTTC GTGGACCCAC GCAAGGACAG TGCCCGCTAT 
GCCCAGGAAA ACTTCAAGTC CTTTGTCTTC ACGGAGACCC AGTTTACAGC TGTGACAGCC 
TATCAGAACC ACAGGATCAC GCAGCTGAAA ATTGCTAGCA ACCCCTTTGC CAAAGGTTTC 
AGAGAGGCTG ACCCAGACTC ATGGCCTGTG ACCCCCCGGC CACTGCTCAG CATCCCTGCC 
CGGAGTCGTA GCAGCCTCAG TCCCTGCCTC CTTAAGGGCT CTGCAGAACG AGAGAAAGAT 
ACCAGTAAAG CTTCCGCTTC CAGCTCCAGG ACCCCTACTC AGCCACACAA TCAGCTGCTG 
CCTGCCCCTG ATGTCCTGCT AGCCCCTGCC ACCTACAGAC CCCTCCCTTA CCAGAACCTG 
TACCCTGGAT CCCCAAGCCG TGCTGGGCCC CCAAGAGCTC GGCTGGCACC TTACCCTCTC 
CCCAACATCA GCACGGCTGG AGATCAGGAG GACCCAACCC TCGCAGCTGG GCTGGGACTC 
CTGCCCACCT CTGCCTTGTG CTTGGTGTCT AATCAAGCCT CGCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TBX10 ( NM_001001320.1 ) cDNA ORF clone, Mus musculus(House mouse) -> NP_001001320.1 Mus musculus T-box 10 (Tbx10), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OMu09480
Clone ID Related Accession (Same CDS sequence)	NM_001001320.1
Accession Version	NM_001001320.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1158bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-03
Organism	Mus musculus(house mouse)
Product	T-box transcription factor TBX10 isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AY229976.1. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AY229976.1, BC109150.2 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849385, SAMN01164134 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCAGTCT TCCTGTCTGC TGGCCTTGGG GTACTTGCTC CTCCAGAGAC CTGTCCCCAT 
CCCGAGACCA GCACCAGCTG GGAGTCCCGG CTGGGGACAC GATTCCCCTC AGGTTCCTGC 
ACCATGTCTA CAGAAGCCCA AGCCATGGCA GAGCCCACTG GGCAGGGCCC CAAGAACCCA 
CGTGTGTCCA GTGTGATGGT GCAGCTGGAG ATGAAGCCAC TGTGGGAAGA GTTCAACCAG 
CTGGGCACAG AGATGATTGT CACCAAAGCG GGCAGGAGGA TGTTCCCAAC GTTCCAGGTG 
AAGATCCTGG GCATGGACAC GCTGGCTGAC TACGCTTTGC TCATGGACTT TATCCCGTTG 
GATGACAAGA GATACAGGTA CGCATTCCAC AGTTCTGCCT GGCTGGTGGC AGGCAAGGCA 
GACCCTGCCA CGCCTGGCAG GGTGCACTTC CACCCTGACT CGCCAGCCAA GGGTGCCCAG 
TGGATGCGGC AGATTGTGTC CTTTGACAAA CTCAAGCTGA CCAACAACCT GATGGACGAC 
AATGGCCACA TCATCCTCAA CTCCATGCAT CGTTATCAGC CCCGTTTCCA CGTGGTCTTC 
GTGGACCCAC GCAAGGACAG TGCCCGCTAT GCCCAGGAAA ACTTCAAGTC CTTTGTCTTC 
ACGGAGACCC AGTTTACAGC TGTGACAGCC TATCAGAACC ACAGGATCAC GCAGCTGAAA 
ATTGCTAGCA ACCCCTTTGC CAAAGGTTTC AGAGAGGCTG ACCCAGACTC ATGGCCTGTG 
ACCCCCCGGC CACTGCTCAG CATCCCTGCC CGGAGTCGTA GCAGCCTCAG TCCCTGCCTC 
CTTAAGGGCT CTGCAGAACG AGAGAAAGAT ACCAGTAAAG CTTCCGCTTC CAGCTCCAGG 
ACCCCTACTC AGCCACACAA TCAGCTGCTG CCTGCCCCTG ATGTCCTGCT AGCCCCTGCC 
ACCTACAGAC CCCTCCCTTA CCAGAACCTG TACCCTGGAT CCCCAAGCCG TGCTGGGCCC 
CCAAGAGCTC GGCTGGCACC TTACCCTCTC CCCAACATCA GCACGGCTGG AGATCAGGAG 
GACCCAACCC TCGCAGCTGG GCTGGGACTC CTGCCCACCT CTGCCTTGTG CTTGGTGTCT 
AATCAAGCCT CGCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001001320.1
CDS	357..1514
Translation	MAVFLSAGLGVLAPPETCPHPETSTSWESRLGTRFPSGSCTMSTEAQAMAEPTGQGPKNPRVSSVMVQLEMKPLWEEFNQLGTEMIVTKAGRRMFPTFQVKILGMDTLADYALLMDFIPLDDKRYRYAFHSSAWLVAGKADPATPGRVHFHPDSPAKGAQWMRQIVSFDKLKLTNNLMDDNGHIILNSMHRYQPRFHVVFVDPRKDSARYAQENFKSFVFTETQFTAVTAYQNHRITQLKIASNPFAKGFREADPDSWPVTPRPLLSIPARSRSSLSPCLLKGSAEREKDTSKASASSSRTPTQPHNQLLPAPDVLLAPATYRPLPYQNLYPGSPSRAGPPRARLAPYPLPNISTAGDQEDPTLAAGLGLLPTSALCLVSNQASQ

Target ORF information:

RefSeq Version	NM_001001320.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus T-box 10 (Tbx10), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001001320.1

ORF Insert Sequence:

ATGGCAGTCT TCCTGTCTGC TGGCCTTGGG GTACTTGCTC CTCCAGAGAC CTGTCCCCAT 
CCCGAGACCA GCACCAGCTG GGAGTCCCGG CTGGGGACAC GATTCCCCTC AGGTTCCTGC 
ACCATGTCTA CAGAAGCCCA AGCCATGGCA GAGCCCACTG GGCAGGGCCC CAAGAACCCA 
CGTGTGTCCA GTGTGATGGT GCAGCTGGAG ATGAAGCCAC TGTGGGAAGA GTTCAACCAG 
CTGGGCACAG AGATGATTGT CACCAAAGCG GGCAGGAGGA TGTTCCCAAC GTTCCAGGTG 
AAGATCCTGG GCATGGACAC GCTGGCTGAC TACGCTTTGC TCATGGACTT TATCCCGTTG 
GATGACAAGA GATACAGGTA CGCATTCCAC AGTTCTGCCT GGCTGGTGGC AGGCAAGGCA 
GACCCTGCCA CGCCTGGCAG GGTGCACTTC CACCCTGACT CGCCAGCCAA GGGTGCCCAG 
TGGATGCGGC AGATTGTGTC CTTTGACAAA CTCAAGCTGA CCAACAACCT GATGGACGAC 
AATGGCCACA TCATCCTCAA CTCCATGCAT CGTTATCAGC CCCGTTTCCA CGTGGTCTTC 
GTGGACCCAC GCAAGGACAG TGCCCGCTAT GCCCAGGAAA ACTTCAAGTC CTTTGTCTTC 
ACGGAGACCC AGTTTACAGC TGTGACAGCC TATCAGAACC ACAGGATCAC GCAGCTGAAA 
ATTGCTAGCA ACCCCTTTGC CAAAGGTTTC AGAGAGGCTG ACCCAGACTC ATGGCCTGTG 
ACCCCCCGGC CACTGCTCAG CATCCCTGCC CGGAGTCGTA GCAGCCTCAG TCCCTGCCTC 
CTTAAGGGCT CTGCAGAACG AGAGAAAGAT ACCAGTAAAG CTTCCGCTTC CAGCTCCAGG 
ACCCCTACTC AGCCACACAA TCAGCTGCTG CCTGCCCCTG ATGTCCTGCT AGCCCCTGCC 
ACCTACAGAC CCCTCCCTTA CCAGAACCTG TACCCTGGAT CCCCAAGCCG TGCTGGGCCC 
CCAAGAGCTC GGCTGGCACC TTACCCTCTC CCCAACATCA GCACGGCTGG AGATCAGGAG 
GACCCAACCC TCGCAGCTGG GCTGGGACTC CTGCCCACCT CTGCCTTGTG CTTGGTGTCT 
AATCAAGCCT CGCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TBX10 ( NM_011533.2 ) cDNA ORF clone, Mus musculus(House mouse) -> NP_035663.1 Mus musculus T-box 10 (Tbx10), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OMu54999
Clone ID Related Accession (Same CDS sequence)	NM_011533.2 , XM_006531632.2
Accession Version	NM_011533.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1035bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-03
Organism	Mus musculus(house mouse)
Product	T-box transcription factor TBX10 isoform 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC133523.3. On Apr 7, 2010 this sequence version replaced NM_011533.1. Transcript Variant: This variant (2) uses an alternate splice site and uses a downstream start codon, compared to variant 1. Isoform 2 has a shorter N-terminus, compared to isoform 1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AY542280.1 [ECO:0000332] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTCTACAG AAGCCCAAGC CATGGCAGAG CCCACTGGGC AGGGCCCCAA GAACCCACGT 
GTGTCCAGTG TGATGGTGCA GCTGGAGATG AAGCCACTGT GGGAAGAGTT CAACCAGCTG 
GGCACAGAGA TGATTGTCAC CAAAGCGGGC AGGAGGATGT TCCCAACGTT CCAGGTGAAG 
ATCCTGGGCA TGGACACGCT GGCTGACTAC GCTTTGCTCA TGGACTTTAT CCCGTTGGAT 
GACAAGAGAT ACAGGTACGC ATTCCACAGT TCTGCCTGGC TGGTGGCAGG CAAGGCAGAC 
CCTGCCACGC CTGGCAGGGT GCACTTCCAC CCTGACTCGC CAGCCAAGGG TGCCCAGTGG 
ATGCGGCAGA TTGTGTCCTT TGACAAACTC AAGCTGACCA ACAACCTGAT GGACGACAAT 
GGCCACATCA TCCTCAACTC CATGCATCGT TATCAGCCCC GTTTCCACGT GGTCTTCGTG 
GACCCACGCA AGGACAGTGC CCGCTATGCC CAGGAAAACT TCAAGTCCTT TGTCTTCACG 
GAGACCCAGT TTACAGCTGT GACAGCCTAT CAGAACCACA GGATCACGCA GCTGAAAATT 
GCTAGCAACC CCTTTGCCAA AGGTTTCAGA GAGGCTGACC CAGACTCATG GCCTGTGACC 
CCCCGGCCAC TGCTCAGCAT CCCTGCCCGG AGTCGTAGCA GCCTCAGTCC CTGCCTCCTT 
AAGGGCTCTG CAGAACGAGA GAAAGATACC AGTAAAGCTT CCGCTTCCAG CTCCAGGACC 
CCTACTCAGC CACACAATCA GCTGCTGCCT GCCCCTGATG TCCTGCTAGC CCCTGCCACC 
TACAGACCCC TCCCTTACCA GAACCTGTAC CCTGGATCCC CAAGCCGTGC TGGGCCCCCA 
AGAGCTCGGC TGGCACCTTA CCCTCTCCCC AACATCAGCA CGGCTGGAGA TCAGGAGGAC 
CCAACCCTCG CAGCTGGGCT GGGACTCCTG CCCACCTCTG CCTTGTGCTT GGTGTCTAAT 
CAAGCCTCGC AGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_035663.1
CDS	323..1357
Translation	MSTEAQAMAEPTGQGPKNPRVSSVMVQLEMKPLWEEFNQLGTEMIVTKAGRRMFPTFQVKILGMDTLADYALLMDFIPLDDKRYRYAFHSSAWLVAGKADPATPGRVHFHPDSPAKGAQWMRQIVSFDKLKLTNNLMDDNGHIILNSMHRYQPRFHVVFVDPRKDSARYAQENFKSFVFTETQFTAVTAYQNHRITQLKIASNPFAKGFREADPDSWPVTPRPLLSIPARSRSSLSPCLLKGSAEREKDTSKASASSSRTPTQPHNQLLPAPDVLLAPATYRPLPYQNLYPGSPSRAGPPRARLAPYPLPNISTAGDQEDPTLAAGLGLLPTSALCLVSNQASQ

Target ORF information:

RefSeq Version	NM_011533.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus T-box 10 (Tbx10), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_011533.2

ORF Insert Sequence:

ATGTCTACAG AAGCCCAAGC CATGGCAGAG CCCACTGGGC AGGGCCCCAA GAACCCACGT 
GTGTCCAGTG TGATGGTGCA GCTGGAGATG AAGCCACTGT GGGAAGAGTT CAACCAGCTG 
GGCACAGAGA TGATTGTCAC CAAAGCGGGC AGGAGGATGT TCCCAACGTT CCAGGTGAAG 
ATCCTGGGCA TGGACACGCT GGCTGACTAC GCTTTGCTCA TGGACTTTAT CCCGTTGGAT 
GACAAGAGAT ACAGGTACGC ATTCCACAGT TCTGCCTGGC TGGTGGCAGG CAAGGCAGAC 
CCTGCCACGC CTGGCAGGGT GCACTTCCAC CCTGACTCGC CAGCCAAGGG TGCCCAGTGG 
ATGCGGCAGA TTGTGTCCTT TGACAAACTC AAGCTGACCA ACAACCTGAT GGACGACAAT 
GGCCACATCA TCCTCAACTC CATGCATCGT TATCAGCCCC GTTTCCACGT GGTCTTCGTG 
GACCCACGCA AGGACAGTGC CCGCTATGCC CAGGAAAACT TCAAGTCCTT TGTCTTCACG 
GAGACCCAGT TTACAGCTGT GACAGCCTAT CAGAACCACA GGATCACGCA GCTGAAAATT 
GCTAGCAACC CCTTTGCCAA AGGTTTCAGA GAGGCTGACC CAGACTCATG GCCTGTGACC 
CCCCGGCCAC TGCTCAGCAT CCCTGCCCGG AGTCGTAGCA GCCTCAGTCC CTGCCTCCTT 
AAGGGCTCTG CAGAACGAGA GAAAGATACC AGTAAAGCTT CCGCTTCCAG CTCCAGGACC 
CCTACTCAGC CACACAATCA GCTGCTGCCT GCCCCTGATG TCCTGCTAGC CCCTGCCACC 
TACAGACCCC TCCCTTACCA GAACCTGTAC CCTGGATCCC CAAGCCGTGC TGGGCCCCCA 
AGAGCTCGGC TGGCACCTTA CCCTCTCCCC AACATCAGCA CGGCTGGAGA TCAGGAGGAC 
CCAACCCTCG CAGCTGGGCT GGGACTCCTG CCCACCTCTG CCTTGTGCTT GGTGTCTAAT 
CAAGCCTCGC AGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TBX10 ( XM_006531632.2 ) cDNA ORF clone, Mus musculus(House mouse) -> XP_006531695.1 Mus musculus T-box 10 (Tbx10), transcript variant X2, mRNA.

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CloneID	OMu54999
Clone ID Related Accession (Same CDS sequence)	NM_011533.2 , XM_006531632.2
Accession Version	XM_006531632.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1035bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-02-08
Organism	Mus musculus(House mouse)
Product	T-box transcription factor TBX10 isoform X1
Comment

ATGTCTACAG AAGCCCAAGC CATGGCAGAG CCCACTGGGC AGGGCCCCAA GAACCCACGT 
GTGTCCAGTG TGATGGTGCA GCTGGAGATG AAGCCACTGT GGGAAGAGTT CAACCAGCTG 
GGCACAGAGA TGATTGTCAC CAAAGCGGGC AGGAGGATGT TCCCAACGTT CCAGGTGAAG 
ATCCTGGGCA TGGACACGCT GGCTGACTAC GCTTTGCTCA TGGACTTTAT CCCGTTGGAT 
GACAAGAGAT ACAGGTACGC ATTCCACAGT TCTGCCTGGC TGGTGGCAGG CAAGGCAGAC 
CCTGCCACGC CTGGCAGGGT GCACTTCCAC CCTGACTCGC CAGCCAAGGG TGCCCAGTGG 
ATGCGGCAGA TTGTGTCCTT TGACAAACTC AAGCTGACCA ACAACCTGAT GGACGACAAT 
GGCCACATCA TCCTCAACTC CATGCATCGT TATCAGCCCC GTTTCCACGT GGTCTTCGTG 
GACCCACGCA AGGACAGTGC CCGCTATGCC CAGGAAAACT TCAAGTCCTT TGTCTTCACG 
GAGACCCAGT TTACAGCTGT GACAGCCTAT CAGAACCACA GGATCACGCA GCTGAAAATT 
GCTAGCAACC CCTTTGCCAA AGGTTTCAGA GAGGCTGACC CAGACTCATG GCCTGTGACC 
CCCCGGCCAC TGCTCAGCAT CCCTGCCCGG AGTCGTAGCA GCCTCAGTCC CTGCCTCCTT 
AAGGGCTCTG CAGAACGAGA GAAAGATACC AGTAAAGCTT CCGCTTCCAG CTCCAGGACC 
CCTACTCAGC CACACAATCA GCTGCTGCCT GCCCCTGATG TCCTGCTAGC CCCTGCCACC 
TACAGACCCC TCCCTTACCA GAACCTGTAC CCTGGATCCC CAAGCCGTGC TGGGCCCCCA 
AGAGCTCGGC TGGCACCTTA CCCTCTCCCC AACATCAGCA CGGCTGGAGA TCAGGAGGAC 
CCAACCCTCG CAGCTGGGCT GGGACTCCTG CCCACCTCTG CCTTGTGCTT GGTGTCTAAT 
CAAGCCTCGC AGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006531695.1
Translation	MSTEAQAMAEPTGQGPKNPRVSSVMVQLEMKPLWEEFNQLGTEMIVTKAGRRMFPTFQVKILGMDTLADYALLMDFIPLDDKRYRYAFHSSAWLVAGKADPATPGRVHFHPDSPAKGAQWMRQIVSFDKLKLTNNLMDDNGHIILNSMHRYQPRFHVVFVDPRKDSARYAQENFKSFVFTETQFTAVTAYQNHRITQLKIASNPFAKGFREADPDSWPVTPRPLLSIPARSRSSLSPCLLKGSAEREKDTSKASASSSRTPTQPHNQLLPAPDVLLAPATYRPLPYQNLYPGSPSRAGPPRARLAPYPLPNISTAGDQEDPTLAAGLGLLPTSALCLVSNQASQ

Target ORF information:

RefSeq Version	XM_006531632.2
Organism	Mus musculus(House mouse)
Definition	Mus musculus T-box 10 (Tbx10), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006531632.2

ORF Insert Sequence:

ATGTCTACAG AAGCCCAAGC CATGGCAGAG CCCACTGGGC AGGGCCCCAA GAACCCACGT 
GTGTCCAGTG TGATGGTGCA GCTGGAGATG AAGCCACTGT GGGAAGAGTT CAACCAGCTG 
GGCACAGAGA TGATTGTCAC CAAAGCGGGC AGGAGGATGT TCCCAACGTT CCAGGTGAAG 
ATCCTGGGCA TGGACACGCT GGCTGACTAC GCTTTGCTCA TGGACTTTAT CCCGTTGGAT 
GACAAGAGAT ACAGGTACGC ATTCCACAGT TCTGCCTGGC TGGTGGCAGG CAAGGCAGAC 
CCTGCCACGC CTGGCAGGGT GCACTTCCAC CCTGACTCGC CAGCCAAGGG TGCCCAGTGG 
ATGCGGCAGA TTGTGTCCTT TGACAAACTC AAGCTGACCA ACAACCTGAT GGACGACAAT 
GGCCACATCA TCCTCAACTC CATGCATCGT TATCAGCCCC GTTTCCACGT GGTCTTCGTG 
GACCCACGCA AGGACAGTGC CCGCTATGCC CAGGAAAACT TCAAGTCCTT TGTCTTCACG 
GAGACCCAGT TTACAGCTGT GACAGCCTAT CAGAACCACA GGATCACGCA GCTGAAAATT 
GCTAGCAACC CCTTTGCCAA AGGTTTCAGA GAGGCTGACC CAGACTCATG GCCTGTGACC 
CCCCGGCCAC TGCTCAGCAT CCCTGCCCGG AGTCGTAGCA GCCTCAGTCC CTGCCTCCTT 
AAGGGCTCTG CAGAACGAGA GAAAGATACC AGTAAAGCTT CCGCTTCCAG CTCCAGGACC 
CCTACTCAGC CACACAATCA GCTGCTGCCT GCCCCTGATG TCCTGCTAGC CCCTGCCACC 
TACAGACCCC TCCCTTACCA GAACCTGTAC CCTGGATCCC CAAGCCGTGC TGGGCCCCCA 
AGAGCTCGGC TGGCACCTTA CCCTCTCCCC AACATCAGCA CGGCTGGAGA TCAGGAGGAC 
CCAACCCTCG CAGCTGGGCT GGGACTCCTG CCCACCTCTG CCTTGTGCTT GGTGTCTAAT 
CAAGCCTCGC AGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

High-throughput discovery of novel developmental phenotypes.
Nature537(7621)508-514(2016 09)
Dickinson ME,Flenniken AM,Ji X,Teboul L,Wong MD,White JK,Meehan TF,Weninger WJ,Westerberg H,Adissu H,Baker CN,Bower L,Brown JM,Caddle LB,Chiani F,Clary D,Cleak J,Daly MJ,Denegre JM,Doe B,Dolan ME,Edie SM,Fuchs H,Gailus-Durner V,Galli A,Gambadoro A,Gallegos J,Guo S,Horner NR,Hsu CW,Johnson SJ,Kalaga S,Keith LC,Lanoue L,Lawson TN,Lek M,Mark M,Marschall S,Mason J,McElwee ML,Newbigging S,Nutter LM,Peterson KA,Ramirez-Solis R,Rowland DJ,Ryder E,Samocha KE,Seavitt JR,Selloum M,Szoke-Kovacs Z,Tamura M,Trainor AG,Tudose I,Wakana S,Warren J,Wendling O,West DB,Wong L,Yoshiki A,,,,,,,,,MacArthur DG,Tocchini-Valentini GP,Gao X,Flicek P,Bradley A,Skarnes WC,Justice MJ,Parkinson HE,Moore M,Wells S,Braun RE,Svenson KL,de Angelis MH,Herault Y,Mohun T,Mallon AM,Henkelman RM,Brown SD,Adams DJ,Lloyd KC,McKerlie C,Beaudet AL,Bu?an M,Murray SA

The International Mouse Phenotyping Consortium Web Portal, a unified point of access for knockout mice and related phenotyping data.
Nucleic acids research42(Database issue)D802-9(2014 Jan)
Koscielny G,Yaikhom G,Iyer V,Meehan TF,Morgan H,Atienza-Herrero J,Blake A,Chen CK,Easty R,Di Fenza A,Fiegel T,Grifiths M,Horne A,Karp NA,Kurbatova N,Mason JC,Matthews P,Oakley DJ,Qazi A,Regnart J,Retha A,Santos LA,Sneddon DJ,Warren J,Westerberg H,Wilson RJ,Melvin DG,Smedley D,Brown SD,Flicek P,Skarnes WC,Mallon AM,Parkinson H

SOX2 regulates the hypothalamic-pituitary axis at multiple levels.
The Journal of clinical investigation122(10)3635-46(2012 Oct)
Jayakody SA,Andoniadou CL,Gaston-Massuet C,Signore M,Cariboni A,Bouloux PM,Le Tissier P,Pevny LH,Dattani MT,Martinez-Barbera JP

A unique expression pattern of Tbx10 in the hindbrain as revealed by Tbx10(LacZ) allele.
Genesis (New York, N.Y. : 2000)48(5)295-302(2010 May)
Xue XD,Kimura W,Wang B,Hikosaka K,Itakura T,Uezato T,Matsuda M,Koseki H,Miura N

A systems approach reveals that the myogenesis genome network is regulated by the transcriptional repressor RP58.
Developmental cell17(6)836-48(2009 Dec)
Yokoyama S,Ito Y,Ueno-Kudoh H,Shimizu H,Uchibe K,Albini S,Mitsuoka K,Miyaki S,Kiso M,Nagai A,Hikata T,Osada T,Fukuda N,Yamashita S,Harada D,Mezzano V,Kasai M,Puri PL,Hayashizaki Y,Okado H,Hashimoto M,Asahara H

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TBX10 cDNA ORF clone, Mus musculus(House mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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