Dio3 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Dio3
Entrez Gene ID	107585
Full Name	deiodinase, iodothyronine type III
General protein information	Preferred Names deiodinase, iodothyronine type III Names thyroxine 5-deiodinase 5DIII DIOIII type 3 DI type III iodothyronine deiodinase type-III 5'-deiodinase
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 12 Map Location: 12\|12 F1
Summary	This is an intronless, imprinted gene that is preferentially expressed from the paternal allele in the mouse fetus. The encoded protein belongs to the iodothyronine deiodinase family, and catalyzes the inactivation of thyroid hormone by inner ring deiodination of the prohormone thyroxine (T4) and the bioactive hormone 3,3',5-triiodothyronine (T3) to inactive metabolites, 3,3',5' triiodothyronine (RT3) and 3,3'-diiodothyronine (T2), respectively. It is highly expressed in placenta, fetal and neonatal tissues, and thought to prevent premature exposure of developing fetal tissues to adult levels of thyroid hormones. It thus plays a critical role in mammalian development by regulating circulating fetal thyroid hormone concentration. Knockout mice lacking this gene exhibit severe abnormalities related to development and reproduction. This protein is a selenoprotein, containing the rare selenocysteine (Sec) amino acid at its active site. Sec is encoded by the UGA codon, which normally signals translation termination. The 3' UTRs of selenoprotein mRNAs contain a conserved stem-loop structure, designated the Sec insertion sequence (SECIS) element, that is necessary for the recognition of UGA as a Sec codon rather than as a stop signal. [provided by RefSeq, Jun 2016].

mRNA and Protein(s)

mRNA	Protein	Name
NM_172119.2	NP_742117.2	thyroxine 5-deiodinase

Pathways from BioSystems

Homologies

Homo sapiens (human)	DIO3	NP_001353.4
Rattus norvegicus (Norway rat)	Dio3	NP_058906.3
Gallus gallus (chicken)	DIO3	NP_001116120.1
Xenopus tropicalis (tropical clawed frog)	dio3	NP_001107139.2
	DIO3	NP_001116121.1
Canis lupus familiaris (dog)	DIO3	NP_001157660.1
Danio rerio (zebrafish)	dio3a	NP_001242932.1
Bos taurus (cattle)	DIO3	NP_001010993.2
Mus musculus (house mouse)	Dio3	NP_742117.2
Danio rerio (zebrafish)	dio3b	NP_001171406.2

Gene Ontology
Bibliography

Related articles in PubMed

Type 3 deiodinase is critical for the maturation and function of the thyroid axis.
Hernandez A, Martinez ME, Fiering S, Galton VA, St Germain D
The Journal of clinical investigation116(2)476-84(2006 Feb)

Asymmetric regulation of imprinting on the maternal and paternal chromosomes at the Dlk1-Gtl2 imprinted cluster on mouse chromosome 12.
Lin SP, Youngson N, Takada S, Seitz H, Reik W, Paulsen M, Cavaille J, Ferguson-Smith AC
Nature genetics35(1)97-102(2003 Sep)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Dio3-/- mice display degeneration of retinal cones, the photoreceptors that mediate daylight and color vision. In Dio2-/- mice, cone function was largely normal but deletion of Dio2 in Dio3-/- mice markedly recovered cone numbers and electroretinogram responses, suggesting counterbalancing roles for both enzymes in cone survival.
Title: Deletion of the Thyroid Hormone-Activating Type 2 Deiodinase Rescues Cone Photoreceptor Degeneration but Not Deafness in Mice Lacking Type 3 Deiodinase.

Gene body methylation of noncoding RNA genes was observed and among these microRNA genes were prominent. Of particular note, observed only in hyperphenylalaninemic animals, was hypomethylation of miRNA genes within the imprinted Dlk1-Dio3 locus on chromosome 12.
Title: DNA methylation in the pathophysiology of hyperphenylalaninemia in the PAH(enu2) mouse model of phenylketonuria.

The marked phenotypic abnormalities observed in the D3-deficient mouse, including perinatal mortality, growth retardation, and central hypothyroidism in adult animals, require expression of MCT8, confirming the interdependent relationship between the TH transport into cells and the deiodination processes.
Title: MCT8 Deficiency in Male Mice Mitigates the Phenotypic Abnormalities Associated With the Absence of a Functional Type 3 Deiodinase.

The brain-specific miR-379/miR-410 gene cluster at the imprinted Dlk1-Dio3 domain is implicated in several aspects of brain development and function.
Title: Deletion of the miR-379/miR-410 gene cluster at the imprinted Dlk1-Dio3 locus enhances anxiety-related behaviour.

DNA methylation regulates genomic imprinted DLK1-Dio3 miRNAs in autoimmune lupus
Title: The Upregulation of Genomic Imprinted DLK1-Dio3 miRNAs in Murine Lupus Is Associated with Global DNA Hypomethylation.

The following Dio3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Dio3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu22688	NM_172119.2 Latest version!	Mus musculus deiodinase, iodothyronine type III (Dio3), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$188.30 $269.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Dio3 ( NM_172119.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_742117.2 Mus musculus deiodinase, iodothyronine type III (Dio3), mRNA.

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CloneID	OMu22688
Clone ID Related Accession (Same CDS sequence)	NM_172119.2
Accession Version	NM_172119.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 915bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-31
Organism	Mus musculus(house mouse)
Product	thyroxine 5-deiodinase
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK140797.1 and BX638763.1. On Aug 8, 2006 this sequence version replaced NM_172119.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript is intronless :: AK140797.1, AK146060.1 [ECO:0000345] ##Evidence-Data-END## ##RefSeq-Attributes-START## imprinted gene :: PMID: 12176332, 12399446 protein contains selenocysteine :: PMID: 9886816 ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGCCTCGCC AGGCCGCCTC GAGGTTGGTG GTCGGAGAAG GTGAAGGGCC CCCGGGGGCT 
TCGGGGCCCG CGGCCACCAT GCTCCGCTCT CTGCTGCTTC ACTCGCTGAG GCTCTGCGCC 
CAGACCGCCT CGTGCCTCGT GCTGTTCCCG CGCTTCCTAG GCACGGCCTT CATGCTCTGG 
CTTTTAGATT TCTTGTGCAT CCGCAAGCAT TTCCTGCGCC GTCGCCATCC TGACCACCCT 
GAGCCCGAAG TAGAGCTCAA CAGTGAAGGC GAGGAGATGC CCCCTGACGA CCCGCCCATA 
TGCGTATCAG ACGACAACCG TCTGTGCACC CTGGCCTCTC TCAAAGCCGT GTGGCATGGC 
CAGAAATTGG ATTTCTTCAA GCAAGCCCAT GAGGGTGGCC CAGCGCCCAA CTCGGAGGTT 
GTCCGACCTG ATGGCTTCCA GAGCCAGCGC ATCCTCGACT ACGCACAAGG GACCCGCCCG 
TTGGTGCTCA ATTTTGGCAG CTGTACCTGA CCACCGTTCA TGGCGCGGAT GAGCGCCTTC 
CAGCGCCTGG TCACCAAGTA CCAGCGCGAC GTTGACTTCC TTATCATCTA CATCGAGGAA 
GCCCACCCAT CCGACGGCTG GGTCACCACA GATTCACCCT ATGTCATCCC CCAGCACCGC 
AGCCTGGAGG ACCGTGTCAG CGCAGCGAGA GTACTACAAC AAGGTGCACC TGGCTGTGCT 
CTGGTCCTGG ACACTATGGC CAACTCTAGC AGTTCCGCAT ATGGTGCCTA TTTTGAGCGC 
CTCTACGTCA TCCAGAGTGG CACCATCATG TACCAGGGAG GCCGTGGCCC CGACGGTTAC 
CAGGTGTCTG AGTTGCGCAC TTGGTTGGAG CGCTATGATG AACAGTTGCA TGGTACTAGG 
CCACATCGAT TCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_742117.2
CDS	3..917
Translation	MPRQAASRLVVGEGEGPPGASGPAATMLRSLLLHSLRLCAQTASCLVLFPRFLGTAFMLWLLDFLCIRKHFLRRRHPDHPEPEVELNSEGEEMPPDDPPICVSDDNRLCTLASLKAVWHGQKLDFFKQAHEGGPAPNSEVVRPDGFQSQRILDYAQGTRPLVLNFGSCT*PPFMARMSAFQRLVTKYQRDVDFLIIYIEEAHPSDGWVTTDSPYVIPQHRSLEDRVSAARVLQQGAPGCALVLDTMANSSSSAYGAYFERLYVIQSGTIMYQGGRGPDGYQVSELRTWLERYDEQLHGTRPHRF

Target ORF information:

RefSeq Version	NM_172119.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus deiodinase, iodothyronine type III (Dio3), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_172119.2

ORF Insert Sequence:

ATGCCTCGCC AGGCCGCCTC GAGGTTGGTG GTCGGAGAAG GTGAAGGGCC CCCGGGGGCT 
TCGGGGCCCG CGGCCACCAT GCTCCGCTCT CTGCTGCTTC ACTCGCTGAG GCTCTGCGCC 
CAGACCGCCT CGTGCCTCGT GCTGTTCCCG CGCTTCCTAG GCACGGCCTT CATGCTCTGG 
CTTTTAGATT TCTTGTGCAT CCGCAAGCAT TTCCTGCGCC GTCGCCATCC TGACCACCCT 
GAGCCCGAAG TAGAGCTCAA CAGTGAAGGC GAGGAGATGC CCCCTGACGA CCCGCCCATA 
TGCGTATCAG ACGACAACCG TCTGTGCACC CTGGCCTCTC TCAAAGCCGT GTGGCATGGC 
CAGAAATTGG ATTTCTTCAA GCAAGCCCAT GAGGGTGGCC CAGCGCCCAA CTCGGAGGTT 
GTCCGACCTG ATGGCTTCCA GAGCCAGCGC ATCCTCGACT ACGCACAAGG GACCCGCCCG 
TTGGTGCTCA ATTTTGGCAG CTGTACCTGA CCACCGTTCA TGGCGCGGAT GAGCGCCTTC 
CAGCGCCTGG TCACCAAGTA CCAGCGCGAC GTTGACTTCC TTATCATCTA CATCGAGGAA 
GCCCACCCAT CCGACGGCTG GGTCACCACA GATTCACCCT ATGTCATCCC CCAGCACCGC 
AGCCTGGAGG ACCGTGTCAG CGCAGCGAGA GTACTACAAC AAGGTGCACC TGGCTGTGCT 
CTGGTCCTGG ACACTATGGC CAACTCTAGC AGTTCCGCAT ATGGTGCCTA TTTTGAGCGC 
CTCTACGTCA TCCAGAGTGG CACCATCATG TACCAGGGAG GCCGTGGCCC CGACGGTTAC 
CAGGTGTCTG AGTTGCGCAC TTGGTTGGAG CGCTATGATG AACAGTTGCA TGGTACTAGG 
CCACATCGAT TCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Type 3 deiodinase is critical for the maturation and function of the thyroid axis.
The Journal of clinical investigation116(2)476-84(2006 Feb)
Hernandez A,Martinez ME,Fiering S,Galton VA,St Germain D

Asymmetric regulation of imprinting on the maternal and paternal chromosomes at the Dlk1-Gtl2 imprinted cluster on mouse chromosome 12.
Nature genetics35(1)97-102(2003 Sep)
Lin SP,Youngson N,Takada S,Seitz H,Reik W,Paulsen M,Cavaille J,Ferguson-Smith AC

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Dio3 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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