Brms1 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Brms1
Entrez Gene ID	107392
Full Name	breast cancer metastasis-suppressor 1
Synonyms	AV003220,AW554636
General protein information	Preferred Names breast cancer metastasis-suppressor 1 Names breast cancer metastasis-suppressor 1 homolog
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 19 Map Location: 19\|19 A

mRNA and Protein(s)

mRNA	Protein	Name
NM_134155.1	NP_598916.1	breast cancer metastasis-suppressor 1 homolog
NM_134155.3	NP_598916.1	breast cancer metastasis-suppressor 1 homolog isoform 1
NM_001374048.1	NP_001360977.1	breast cancer metastasis-suppressor 1 homolog isoform 2
XM_006531625.3	XP_006531688.1	breast cancer metastasis-suppressor 1 homolog isoform X3
XM_006531623.3	XP_006531686.1	breast cancer metastasis-suppressor 1 homolog isoform X1
NM_134155.2	NP_598916.1	breast cancer metastasis-suppressor 1 homolog
XM_006531624.3	XP_006531687.1	breast cancer metastasis-suppressor 1 homolog isoform X2
NM_001374047.1	NP_001360976.1	breast cancer metastasis-suppressor 1 homolog isoform 1

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Brms1	NP_598916.1
Homo sapiens (human)	BRMS1	NP_001020128.1
Pan troglodytes (chimpanzee)	BRMS1	XP_001171289.1
Bos taurus (cattle)	BRMS1	NP_001069872.1
Danio rerio (zebrafish)	brms1	NP_956391.1
	BRMS1	XP_001110378.1
Canis lupus familiaris (dog)	BRMS1	XP_005631496.1

Gene Ontology
Bibliography

Related articles in PubMed

High-throughput discovery of novel developmental phenotypes.
Dickinson ME, Flenniken AM, Ji X, Teboul L, Wong MD, White JK, Meehan TF, Weninger WJ, Westerberg H, Adissu H, Baker CN, Bower L, Brown JM, Caddle LB, Chiani F, Clary D, Cleak J, Daly MJ, Denegre JM, Doe B, Dolan ME, Edie SM, Fuchs H, Gailus-Durner V, Galli A, Gambadoro A, Gallegos J, Guo S, Horner NR, Hsu CW, Johnson SJ, Kalaga S, Keith LC, Lanoue L, Lawson TN, Lek M, Mark M, Marschall S, Mason J, McElwee ML, Newbigging S, Nutter LM, Peterson KA, Ramirez-Solis R, Rowland DJ, Ryder E, Samocha KE, Seavitt JR, Selloum M, Szoke-Kovacs Z, Tamura M, Trainor AG, Tudose I, Wakana S, Warren J, Wendling O, West DB, Wong L, Yoshiki A, , , , , , , , , MacArthur DG, Tocchini-Valentini GP, Gao X, Flicek P, Bradley A, Skarnes WC, Justice MJ, Parkinson HE, Moore M, Wells S, Braun RE, Svenson KL, de Angelis MH, Herault Y, Mohun T, Mallon AM, Henkelman RM, Brown SD, Adams DJ, Lloyd KC, McKerlie C, Beaudet AL, Bu?an M, Murray SA
Nature537(7621)508-514(2016 09)

Ubiquitous Brms1 expression is critical for mammary carcinoma metastasis suppression via promotion of apoptosis.
Cook LM, Cao X, Dowell AE, Debies MT, Edmonds MD, Beck BH, Kesterson RA, Desmond RA, Frost AR, Hurst DR, Welch DR
Clinical & experimental metastasis29(4)315-25(2012 Apr)

Identification of essential sequences for cellular localization in BRMS1 metastasis suppressor.
Rivera J, Meg?as D, Navas C, Bravo J
PloS one4(7)e6433(2009 Jul)

Suppression of murine mammary carcinoma metastasis by the murine ortholog of breast cancer metastasis suppressor 1 (Brms1).
Samant RS, Debies MT, Hurst DR, Moore BP, Shevde LA, Welch DR
Cancer letters235(2)260-5(2006 Apr)

Breast cancer metastasis suppressor 1 (BRMS1) forms complexes with retinoblastoma-binding protein 1 (RBP1) and the mSin3 histone deacetylase complex and represses transcription.
Meehan WJ, Samant RS, Hopper JE, Carrozza MJ, Shevde LA, Workman JL, Eckert KA, Verderame MF, Welch DR
The Journal of biological chemistry279(2)1562-9(2004 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The Brms1 suppressed pulmonary metastasis and promoted apoptosis of tumor cells located in the lungs but not in the mammary glands.
Title: Ubiquitous Brms1 expression is critical for mammary carcinoma metastasis suppression via promotion of apoptosis.

results show for the first time that BRMS1 contains both nuclear import and export signals enabling its nucleo-cytoplasmic shuttling
Title: Identification of essential sequences for cellular localization in BRMS1 metastasis suppressor.

BRMS1 may participate in transcriptional regulation via interaction with the mSin3.HDAC complex
Title: Breast cancer metastasis suppressor 1 (BRMS1) forms complexes with retinoblastoma-binding protein 1 (RBP1) and the mSin3 histone deacetylase complex and represses transcription.

Comparative sequence analysis in eight inbred strains
Title: Comparative sequence analysis in eight inbred strains of the metastasis modifier QTL candidate gene Brms1.

The following Brms1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Brms1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu54992	XM_006531623.3 Latest version!	Mus musculus breast cancer metastasis-suppressor 1 (Brms1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
OMu54993	XM_006531624.3 Latest version!	Mus musculus breast cancer metastasis-suppressor 1 (Brms1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$139.30 $199.00
OMu54994	XM_006531625.3 Latest version!	Mus musculus breast cancer metastasis-suppressor 1 (Brms1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$139.30 $199.00
OMu10599	NM_134155.3 Latest version!	Mus musculus breast cancer metastasis-suppressor 1 (Brms1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$139.30 $199.00
OMu10599	NM_001374047.1 Latest version!	Mus musculus breast cancer metastasis-suppressor 1 (Brms1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$139.30 $199.00
OMu90564	NM_001374048.1 Latest version!	Mus musculus breast cancer metastasis-suppressor 1 (Brms1), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$139.30 $199.00
View Old Accession Versions >>
OMu10599	NM_134155.1	Mus musculus breast cancer metastasis-suppressor 1 (Brms1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$188.30 $269.00
OMu10599	NM_134155.2	Mus musculus breast cancer metastasis-suppressor 1 (Brms1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$188.30 $269.00
Hide Old Accession Versions >>

ORF Online Only Promotion

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Brms1 ( NM_134155.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_598916.1 Mus musculus breast cancer metastasis-suppressor 1 (Brms1), mRNA.

Price & Availability↑

CloneID	OMu10599
Clone ID Related Accession (Same CDS sequence)	NM_134155.1 , NM_134155.3 , NM_134155.2 , NM_001374047.1
Accession Version	NM_134155.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 741bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-12-26
Organism	Mus musculus(house mouse)
Product	breast cancer metastasis-suppressor 1 homolog
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC016108.1. ##Evidence-Data-START## Transcript exon combination :: BC016108.1, AF233580.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END##

ATGCCCATCC AGCCTTCAGG CAAAGAAACA GAAGAGATGG AGGCAGAAGG GGATTCAGCA 
GCTGAGATGA ACGGGGAGGC AGATGAGAGC GAGGAGGAGC GGAGTGGCAG CCAAACTGAG 
TCAGAGGAGG AGAGCTCAGA GATGGATGAT GAAGACTATG AGCGGCGCCG CAGTGAGTGT 
GTCAGTGAGA TGCTGGACCT GGAAAAGCAG TTCTCAGAGC TGAAGGAGAA GTTGTTCAGG 
GAACGGCTCA GCCAGCTACG TTTACGACTG GAGGAAGTGG GGGCTGAGAG AGCCCCAGAA 
TATACGGAAC CTCTTGGCGG GCTGCAGCAG AGCCTGAAGA TCCGCATTCA GGTGGCAGGG 
ATCTACAAGG GCTTCTGTCT GGATGTGATC AGGAATAAGT ATGAGTGTGA ACTCCAGGGA 
GCCAAACAAC ACCTGGAGAG TGAGAAGATG CTGCTGTATG ACACACTGCT TGGGGAGCTG 
CAGGAGCGGA TCCAGAGGCT GGAGGAGGAC CGGCAGAGCC TTGACATCAG CTCTGAGTGG 
TGGGACGACA AACTACATAG CAGAAGCAGC TCCAAGGCCG GGGACGCCAT GCCACCCAGC 
AAGAGGAAGA AGGCACCGCT CGTTTCTGGT CCATACATTG TGTACATGCT GCAAGAGATC 
GACATCCTGG AAGACTGGAC AGCCATCAAA AAGGCCAGGG CAGCAGTGTC CCCTCAGAAG 
AGAAAAGCAG ACGGACCCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_598916.1
CDS	95..835
Translation	MPIQPSGKETEEMEAEGDSAAEMNGEADESEEERSGSQTESEEESSEMDDEDYERRRSECVSEMLDLEKQFSELKEKLFRERLSQLRLRLEEVGAERAPEYTEPLGGLQQSLKIRIQVAGIYKGFCLDVIRNKYECELQGAKQHLESEKMLLYDTLLGELQERIQRLEEDRQSLDISSEWWDDKLHSRSSSKAGDAMPPSKRKKAPLVSGPYIVYMLQEIDILEDWTAIKKARAAVSPQKRKADGP

Target ORF information:

RefSeq Version	NM_134155.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus breast cancer metastasis-suppressor 1 (Brms1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_134155.1

ORF Insert Sequence:

ATGCCCATCC AGCCTTCAGG CAAAGAAACA GAAGAGATGG AGGCAGAAGG GGATTCAGCA 
GCTGAGATGA ACGGGGAGGC AGATGAGAGC GAGGAGGAGC GGAGTGGCAG CCAAACTGAG 
TCAGAGGAGG AGAGCTCAGA GATGGATGAT GAAGACTATG AGCGGCGCCG CAGTGAGTGT 
GTCAGTGAGA TGCTGGACCT GGAAAAGCAG TTCTCAGAGC TGAAGGAGAA GTTGTTCAGG 
GAACGGCTCA GCCAGCTACG TTTACGACTG GAGGAAGTGG GGGCTGAGAG AGCCCCAGAA 
TATACGGAAC CTCTTGGCGG GCTGCAGCAG AGCCTGAAGA TCCGCATTCA GGTGGCAGGG 
ATCTACAAGG GCTTCTGTCT GGATGTGATC AGGAATAAGT ATGAGTGTGA ACTCCAGGGA 
GCCAAACAAC ACCTGGAGAG TGAGAAGATG CTGCTGTATG ACACACTGCT TGGGGAGCTG 
CAGGAGCGGA TCCAGAGGCT GGAGGAGGAC CGGCAGAGCC TTGACATCAG CTCTGAGTGG 
TGGGACGACA AACTACATAG CAGAAGCAGC TCCAAGGCCG GGGACGCCAT GCCACCCAGC 
AAGAGGAAGA AGGCACCGCT CGTTTCTGGT CCATACATTG TGTACATGCT GCAAGAGATC 
GACATCCTGG AAGACTGGAC AGCCATCAAA AAGGCCAGGG CAGCAGTGTC CCCTCAGAAG 
AGAAAAGCAG ACGGACCCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Brms1 ( NM_134155.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_598916.1 Mus musculus breast cancer metastasis-suppressor 1 (Brms1), mRNA.

Price & Availability↑

CloneID	OMu10599
Clone ID Related Accession (Same CDS sequence)	NM_134155.1 , NM_134155.3 , NM_134155.2 , NM_001374047.1
Accession Version	NM_134155.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 741bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-09-29
Organism	Mus musculus(house mouse)
Product	breast cancer metastasis-suppressor 1 homolog
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BY234808.1 and BC016108.1. On Mar 31, 2018 this sequence version replaced NM_134155.1. ##Evidence-Data-START## Transcript exon combination :: BC016108.1, AF233580.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCCCATCC AGCCTTCAGG CAAAGAAACA GAAGAGATGG AGGCAGAAGG GGATTCAGCA 
GCTGAGATGA ACGGGGAGGC AGATGAGAGC GAGGAGGAGC GGAGTGGCAG CCAAACTGAG 
TCAGAGGAGG AGAGCTCAGA GATGGATGAT GAAGACTATG AGCGGCGCCG CAGTGAGTGT 
GTCAGTGAGA TGCTGGACCT GGAAAAGCAG TTCTCAGAGC TGAAGGAGAA GTTGTTCAGG 
GAACGGCTCA GCCAGCTACG TTTACGACTG GAGGAAGTGG GGGCTGAGAG AGCCCCAGAA 
TATACGGAAC CTCTTGGCGG GCTGCAGCAG AGCCTGAAGA TCCGCATTCA GGTGGCAGGG 
ATCTACAAGG GCTTCTGTCT GGATGTGATC AGGAATAAGT ATGAGTGTGA ACTCCAGGGA 
GCCAAACAAC ACCTGGAGAG TGAGAAGATG CTGCTGTATG ACACACTGCT TGGGGAGCTG 
CAGGAGCGGA TCCAGAGGCT GGAGGAGGAC CGGCAGAGCC TTGACATCAG CTCTGAGTGG 
TGGGACGACA AACTACATAG CAGAAGCAGC TCCAAGGCCG GGGACGCCAT GCCACCCAGC 
AAGAGGAAGA AGGCACCGCT CGTTTCTGGT CCATACATTG TGTACATGCT GCAAGAGATC 
GACATCCTGG AAGACTGGAC AGCCATCAAA AAGGCCAGGG CAGCAGTGTC CCCTCAGAAG 
AGAAAAGCAG ACGGACCCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_598916.1
CDS	145..885
Translation	MPIQPSGKETEEMEAEGDSAAEMNGEADESEEERSGSQTESEEESSEMDDEDYERRRSECVSEMLDLEKQFSELKEKLFRERLSQLRLRLEEVGAERAPEYTEPLGGLQQSLKIRIQVAGIYKGFCLDVIRNKYECELQGAKQHLESEKMLLYDTLLGELQERIQRLEEDRQSLDISSEWWDDKLHSRSSSKAGDAMPPSKRKKAPLVSGPYIVYMLQEIDILEDWTAIKKARAAVSPQKRKADGP

Target ORF information:

RefSeq Version	NM_134155.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus breast cancer metastasis-suppressor 1 (Brms1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_134155.2

ORF Insert Sequence:

ATGCCCATCC AGCCTTCAGG CAAAGAAACA GAAGAGATGG AGGCAGAAGG GGATTCAGCA 
GCTGAGATGA ACGGGGAGGC AGATGAGAGC GAGGAGGAGC GGAGTGGCAG CCAAACTGAG 
TCAGAGGAGG AGAGCTCAGA GATGGATGAT GAAGACTATG AGCGGCGCCG CAGTGAGTGT 
GTCAGTGAGA TGCTGGACCT GGAAAAGCAG TTCTCAGAGC TGAAGGAGAA GTTGTTCAGG 
GAACGGCTCA GCCAGCTACG TTTACGACTG GAGGAAGTGG GGGCTGAGAG AGCCCCAGAA 
TATACGGAAC CTCTTGGCGG GCTGCAGCAG AGCCTGAAGA TCCGCATTCA GGTGGCAGGG 
ATCTACAAGG GCTTCTGTCT GGATGTGATC AGGAATAAGT ATGAGTGTGA ACTCCAGGGA 
GCCAAACAAC ACCTGGAGAG TGAGAAGATG CTGCTGTATG ACACACTGCT TGGGGAGCTG 
CAGGAGCGGA TCCAGAGGCT GGAGGAGGAC CGGCAGAGCC TTGACATCAG CTCTGAGTGG 
TGGGACGACA AACTACATAG CAGAAGCAGC TCCAAGGCCG GGGACGCCAT GCCACCCAGC 
AAGAGGAAGA AGGCACCGCT CGTTTCTGGT CCATACATTG TGTACATGCT GCAAGAGATC 
GACATCCTGG AAGACTGGAC AGCCATCAAA AAGGCCAGGG CAGCAGTGTC CCCTCAGAAG 
AGAAAAGCAG ACGGACCCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Brms1 ( XM_006531623.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006531686.1 Mus musculus breast cancer metastasis-suppressor 1 (Brms1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu54992
Clone ID Related Accession (Same CDS sequence)	XM_006531623.3
Accession Version	XM_006531623.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1017bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	breast cancer metastasis-suppressor 1 homolog isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000085.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006531623.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACGCACA CGGAAGCGTC GACACCTCCT GGAGAAAGAG AATCCTGCGC AGCACTGTTC 
CGGAGATCAA CGGCGGCGGC GTTCACACCC TGGGTCGGAC AGCTCTCCGG GAGTCCTGGA 
CGTGAGGCCG GAGGCCCCAG TGTCTGCGGT GCGTATGGCC ACGCCCGGCG CTGGGTTGGG 
CTTTCCGGAT CGGAGCTAAA GCAGAGACTT AACCGGCCCG ACTCGCGCGC TGGCCTGAGA 
GGAGCCCGCG GTTCCGAGGG TGGATCGAGA GCCCAGATGC CCATCCAGCC TTCAGGCAAA 
GAAACAGAAG AGATGGAGGC AGAAGGGGAT TCAGCAGCTG AGATGAACGG GGAGGCAGAT 
GAGAGCGAGG AGGAGCGGAG TGGCAGCCAA ACTGAGTCAG AGGAGGAGAG CTCAGAGATG 
GATGATGAAG ACTATGAGCG GCGCCGCAGT GAGTGTGTCA GTGAGATGCT GGACCTGGAA 
AAGCAGTTCT CAGAGCTGAA GGAGAAGTTG TTCAGGGAAC GGCTCAGCCA GCTACGTTTA 
CGACTGGAGG AAGTGGGGGC TGAGAGAGCC CCAGAATATA CGGAACCTCT TGGCGGGCTG 
CAGCAGAGCC TGAAGATCCG CATTCAGGTG GCAGGGATCT ACAAGGGCTT CTGTCTGGAT 
GTGATCAGGA ATAAGTATGA GTGTGAACTC CAGGGAGCCA AACAACACCT GGAGAGTGAG 
AAGATGCTGC TGTATGACAC ACTGCTTGGG GAGCTGCAGG AGCGGATCCA GAGGCTGGAG 
GAGGACCGGC AGAGCCTTGA CATCAGCTCT GAGTGGTGGG ACGACAAACT ACATAGCAGA 
AGCAGCTCCA AGGCCGGGGA CGCCATGCCA CCCAGCAAGA GGAAGAAGGC ACCGCTCGTT 
TCTGGTCCAT ACATTGTGTA CATGCTGCAA GAGATCGACA TCCTGGAAGA CTGGACAGCC 
ATCAAAAAGG CCAGGGCAGC AGTGTCCCCT CAGAAGAGAA AAGCAGACGG ACCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006531686.1
CDS	112..1128
Translation	MTHTEASTPPGERESCAALFRRSTAAAFTPWVGQLSGSPGREAGGPSVCGAYGHARRWVGLSGSELKQRLNRPDSRAGLRGARGSEGGSRAQMPIQPSGKETEEMEAEGDSAAEMNGEADESEEERSGSQTESEEESSEMDDEDYERRRSECVSEMLDLEKQFSELKEKLFRERLSQLRLRLEEVGAERAPEYTEPLGGLQQSLKIRIQVAGIYKGFCLDVIRNKYECELQGAKQHLESEKMLLYDTLLGELQERIQRLEEDRQSLDISSEWWDDKLHSRSSSKAGDAMPPSKRKKAPLVSGPYIVYMLQEIDILEDWTAIKKARAAVSPQKRKADGP

Target ORF information:

RefSeq Version	XM_006531623.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus breast cancer metastasis-suppressor 1 (Brms1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006531623.3

ORF Insert Sequence:

ATGACGCACA CGGAAGCGTC GACACCTCCT GGAGAAAGAG AATCCTGCGC AGCACTGTTC 
CGGAGATCAA CGGCGGCGGC GTTCACACCC TGGGTCGGAC AGCTCTCCGG GAGTCCTGGA 
CGTGAGGCCG GAGGCCCCAG TGTCTGCGGT GCGTATGGCC ACGCCCGGCG CTGGGTTGGG 
CTTTCCGGAT CGGAGCTAAA GCAGAGACTT AACCGGCCCG ACTCGCGCGC TGGCCTGAGA 
GGAGCCCGCG GTTCCGAGGG TGGATCGAGA GCCCAGATGC CCATCCAGCC TTCAGGCAAA 
GAAACAGAAG AGATGGAGGC AGAAGGGGAT TCAGCAGCTG AGATGAACGG GGAGGCAGAT 
GAGAGCGAGG AGGAGCGGAG TGGCAGCCAA ACTGAGTCAG AGGAGGAGAG CTCAGAGATG 
GATGATGAAG ACTATGAGCG GCGCCGCAGT GAGTGTGTCA GTGAGATGCT GGACCTGGAA 
AAGCAGTTCT CAGAGCTGAA GGAGAAGTTG TTCAGGGAAC GGCTCAGCCA GCTACGTTTA 
CGACTGGAGG AAGTGGGGGC TGAGAGAGCC CCAGAATATA CGGAACCTCT TGGCGGGCTG 
CAGCAGAGCC TGAAGATCCG CATTCAGGTG GCAGGGATCT ACAAGGGCTT CTGTCTGGAT 
GTGATCAGGA ATAAGTATGA GTGTGAACTC CAGGGAGCCA AACAACACCT GGAGAGTGAG 
AAGATGCTGC TGTATGACAC ACTGCTTGGG GAGCTGCAGG AGCGGATCCA GAGGCTGGAG 
GAGGACCGGC AGAGCCTTGA CATCAGCTCT GAGTGGTGGG ACGACAAACT ACATAGCAGA 
AGCAGCTCCA AGGCCGGGGA CGCCATGCCA CCCAGCAAGA GGAAGAAGGC ACCGCTCGTT 
TCTGGTCCAT ACATTGTGTA CATGCTGCAA GAGATCGACA TCCTGGAAGA CTGGACAGCC 
ATCAAAAAGG CCAGGGCAGC AGTGTCCCCT CAGAAGAGAA AAGCAGACGG ACCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Brms1 ( XM_006531624.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006531687.1 Mus musculus breast cancer metastasis-suppressor 1 (Brms1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OMu54993
Clone ID Related Accession (Same CDS sequence)	XM_006531624.3
Accession Version	XM_006531624.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 924bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	breast cancer metastasis-suppressor 1 homolog isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000085.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006531624.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACGCACA CGGAAGCGTC GACACCTCCT GGAGAAAGAG AATCCTGCGC AGCACTGTTC 
CGGAGATCAA CGGCGGCGGC GTTCACACCC TGGGTCGGAC AGCTCTCCGG GAGTCCTGGA 
CGTGAGGCCG GAGGCCCCAG TGTCTGCGGT GCGTATGGCC ACGCCCGGCG CTGGGTTGGG 
CTTTCCGGAT CGGAGCTAAA GCAGAGACTT AACCGGCCCG ACTCGCGCGC TGGCCTGAGA 
GGAGCCCGCG GTTCCGAGGG TGGATCGAGA GCCCAGATGC CCATCCAGCC TTCAGGCAAA 
GAAACAGAAG AGATGGAGGC AGAAGGGGAT TCAGCAGCTG AGATGAACGG GGAGGCAGAT 
GAGAGCGAGG AGGAGCGGAG TGGCAGCCAA ACTGAGTCAG AGGAGGAGAG CTCAGAGATG 
GATGATGAAG ACTATGAGCG GCGCCGCAGT GAGTGTGTCA GTGAGATGCT GGACCTGGAA 
AAGCAGTTCT CAGAGCTGAA GGAGAAGTTG TTCAGGGAAC GGCTCAGCCA GCTACGTTTA 
CGACTGGAGG AAGTGGGGGC TGAGAGAGCC CCAGAATATA CGGAACCTCT TGGCGGGCTG 
CAGCAGAGCC TGAAGATCCG CATTCAGGTG GCAGGGATCT ACAAGGGCTT CTGTCTGGAT 
GTGATCAGGA ATAAGTATGA GTGTGAACTC CAGGGAGCCA AACAACACCT GGAGAGTGAG 
AAGATGCTGC TGTATGACAC ACTGCTTGGG GAGCTGCAGG AGCGGATCCA GAGGCTGGAG 
GAGGACCGGC AGAGCCTTGA CATCAGCTCT GGGCGTCAGT GTTCATATGG GATCCACAAA 
TTTCCTTCAT CCCCATCTAC AAAGCAAATA GAAGACCTAA GGCTTAAGCA AAGAAGGGCA 
GTGGTCTTCC CCAGGCTGCA CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006531687.1
CDS	112..1035
Translation	MTHTEASTPPGERESCAALFRRSTAAAFTPWVGQLSGSPGREAGGPSVCGAYGHARRWVGLSGSELKQRLNRPDSRAGLRGARGSEGGSRAQMPIQPSGKETEEMEAEGDSAAEMNGEADESEEERSGSQTESEEESSEMDDEDYERRRSECVSEMLDLEKQFSELKEKLFRERLSQLRLRLEEVGAERAPEYTEPLGGLQQSLKIRIQVAGIYKGFCLDVIRNKYECELQGAKQHLESEKMLLYDTLLGELQERIQRLEEDRQSLDISSGRQCSYGIHKFPSSPSTKQIEDLRLKQRRAVVFPRLH

Target ORF information:

RefSeq Version	XM_006531624.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus breast cancer metastasis-suppressor 1 (Brms1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006531624.3

ORF Insert Sequence:

ATGACGCACA CGGAAGCGTC GACACCTCCT GGAGAAAGAG AATCCTGCGC AGCACTGTTC 
CGGAGATCAA CGGCGGCGGC GTTCACACCC TGGGTCGGAC AGCTCTCCGG GAGTCCTGGA 
CGTGAGGCCG GAGGCCCCAG TGTCTGCGGT GCGTATGGCC ACGCCCGGCG CTGGGTTGGG 
CTTTCCGGAT CGGAGCTAAA GCAGAGACTT AACCGGCCCG ACTCGCGCGC TGGCCTGAGA 
GGAGCCCGCG GTTCCGAGGG TGGATCGAGA GCCCAGATGC CCATCCAGCC TTCAGGCAAA 
GAAACAGAAG AGATGGAGGC AGAAGGGGAT TCAGCAGCTG AGATGAACGG GGAGGCAGAT 
GAGAGCGAGG AGGAGCGGAG TGGCAGCCAA ACTGAGTCAG AGGAGGAGAG CTCAGAGATG 
GATGATGAAG ACTATGAGCG GCGCCGCAGT GAGTGTGTCA GTGAGATGCT GGACCTGGAA 
AAGCAGTTCT CAGAGCTGAA GGAGAAGTTG TTCAGGGAAC GGCTCAGCCA GCTACGTTTA 
CGACTGGAGG AAGTGGGGGC TGAGAGAGCC CCAGAATATA CGGAACCTCT TGGCGGGCTG 
CAGCAGAGCC TGAAGATCCG CATTCAGGTG GCAGGGATCT ACAAGGGCTT CTGTCTGGAT 
GTGATCAGGA ATAAGTATGA GTGTGAACTC CAGGGAGCCA AACAACACCT GGAGAGTGAG 
AAGATGCTGC TGTATGACAC ACTGCTTGGG GAGCTGCAGG AGCGGATCCA GAGGCTGGAG 
GAGGACCGGC AGAGCCTTGA CATCAGCTCT GGGCGTCAGT GTTCATATGG GATCCACAAA 
TTTCCTTCAT CCCCATCTAC AAAGCAAATA GAAGACCTAA GGCTTAAGCA AAGAAGGGCA 
GTGGTCTTCC CCAGGCTGCA CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Brms1 ( XM_006531625.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006531688.1 Mus musculus breast cancer metastasis-suppressor 1 (Brms1), transcript variant X3, mRNA.

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CloneID	OMu54994
Clone ID Related Accession (Same CDS sequence)	XM_006531625.3
Accession Version	XM_006531625.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 648bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	breast cancer metastasis-suppressor 1 homolog isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000085.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006531625.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCCATCC AGCCTTCAGG CAAAGAAACA GAAGAGATGG AGGCAGAAGG GGATTCAGCA 
GCTGAGATGA ACGGGGAGGC AGATGAGAGC GAGGAGGAGC GGAGTGGCAG CCAAACTGAG 
TCAGAGGAGG AGAGCTCAGA GATGGATGAT GAAGACTATG AGCGGCGCCG CAGTGAGTGT 
GTCAGTGAGA TGCTGGACCT GGAAAAGCAG TTCTCAGAGC TGAAGGAGAA GTTGTTCAGG 
GAACGGCTCA GCCAGCTACG TTTACGACTG GAGGAAGTGG GGGCTGAGAG AGCCCCAGAA 
TATACGGAAC CTCTTGGCGG GCTGCAGCAG AGCCTGAAGA TCCGCATTCA GGTGGCAGGG 
ATCTACAAGG GCTTCTGTCT GGATGTGATC AGGAATAAGT ATGAGTGTGA ACTCCAGGGA 
GCCAAACAAC ACCTGGAGAG TGAGAAGATG CTGCTGTATG ACACACTGCT TGGGGAGCTG 
CAGGAGCGGA TCCAGAGGCT GGAGGAGGAC CGGCAGAGCC TTGACATCAG CTCTGGGCGT 
CAGTGTTCAT ATGGGATCCA CAAATTTCCT TCATCCCCAT CTACAAAGCA AATAGAAGAC 
CTAAGGCTTA AGCAAAGAAG GGCAGTGGTC TTCCCCAGGC TGCACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006531688.1
CDS	145..792
Translation	MPIQPSGKETEEMEAEGDSAAEMNGEADESEEERSGSQTESEEESSEMDDEDYERRRSECVSEMLDLEKQFSELKEKLFRERLSQLRLRLEEVGAERAPEYTEPLGGLQQSLKIRIQVAGIYKGFCLDVIRNKYECELQGAKQHLESEKMLLYDTLLGELQERIQRLEEDRQSLDISSGRQCSYGIHKFPSSPSTKQIEDLRLKQRRAVVFPRLH

Target ORF information:

RefSeq Version	XM_006531625.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus breast cancer metastasis-suppressor 1 (Brms1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006531625.3

ORF Insert Sequence:

ATGCCCATCC AGCCTTCAGG CAAAGAAACA GAAGAGATGG AGGCAGAAGG GGATTCAGCA 
GCTGAGATGA ACGGGGAGGC AGATGAGAGC GAGGAGGAGC GGAGTGGCAG CCAAACTGAG 
TCAGAGGAGG AGAGCTCAGA GATGGATGAT GAAGACTATG AGCGGCGCCG CAGTGAGTGT 
GTCAGTGAGA TGCTGGACCT GGAAAAGCAG TTCTCAGAGC TGAAGGAGAA GTTGTTCAGG 
GAACGGCTCA GCCAGCTACG TTTACGACTG GAGGAAGTGG GGGCTGAGAG AGCCCCAGAA 
TATACGGAAC CTCTTGGCGG GCTGCAGCAG AGCCTGAAGA TCCGCATTCA GGTGGCAGGG 
ATCTACAAGG GCTTCTGTCT GGATGTGATC AGGAATAAGT ATGAGTGTGA ACTCCAGGGA 
GCCAAACAAC ACCTGGAGAG TGAGAAGATG CTGCTGTATG ACACACTGCT TGGGGAGCTG 
CAGGAGCGGA TCCAGAGGCT GGAGGAGGAC CGGCAGAGCC TTGACATCAG CTCTGGGCGT 
CAGTGTTCAT ATGGGATCCA CAAATTTCCT TCATCCCCAT CTACAAAGCA AATAGAAGAC 
CTAAGGCTTA AGCAAAGAAG GGCAGTGGTC TTCCCCAGGC TGCACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Brms1 ( NM_134155.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_598916.1 Mus musculus breast cancer metastasis-suppressor 1 (Brms1), transcript variant 1, mRNA.

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CloneID	OMu10599
Clone ID Related Accession (Same CDS sequence)	NM_134155.1 , NM_134155.3 , NM_134155.2 , NM_001374047.1
Accession Version	NM_134155.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 741bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-09
Organism	Mus musculus(house mouse)
Product	breast cancer metastasis-suppressor 1 homolog isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC124502.4. On Sep 10, 2019 this sequence version replaced NM_134155.2. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: AF233580.1, BC016108.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCCCATCC AGCCTTCAGG CAAAGAAACA GAAGAGATGG AGGCAGAAGG GGATTCAGCA 
GCTGAGATGA ACGGGGAGGC AGATGAGAGC GAGGAGGAGC GGAGTGGCAG CCAAACTGAG 
TCAGAGGAGG AGAGCTCAGA GATGGATGAT GAAGACTATG AGCGGCGCCG CAGTGAGTGT 
GTCAGTGAGA TGCTGGACCT GGAAAAGCAG TTCTCAGAGC TGAAGGAGAA GTTGTTCAGG 
GAACGGCTCA GCCAGCTACG TTTACGACTG GAGGAAGTGG GGGCTGAGAG AGCCCCAGAA 
TATACGGAAC CTCTTGGCGG GCTGCAGCAG AGCCTGAAGA TCCGCATTCA GGTGGCAGGG 
ATCTACAAGG GCTTCTGTCT GGATGTGATC AGGAATAAGT ATGAGTGTGA ACTCCAGGGA 
GCCAAACAAC ACCTGGAGAG TGAGAAGATG CTGCTGTATG ACACACTGCT TGGGGAGCTG 
CAGGAGCGGA TCCAGAGGCT GGAGGAGGAC CGGCAGAGCC TTGACATCAG CTCTGAGTGG 
TGGGACGACA AACTACATAG CAGAAGCAGC TCCAAGGCCG GGGACGCCAT GCCACCCAGC 
AAGAGGAAGA AGGCACCGCT CGTTTCTGGT CCATACATTG TGTACATGCT GCAAGAGATC 
GACATCCTGG AAGACTGGAC AGCCATCAAA AAGGCCAGGG CAGCAGTGTC CCCTCAGAAG 
AGAAAAGCAG ACGGACCCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_598916.1
CDS	136..876
Translation	MPIQPSGKETEEMEAEGDSAAEMNGEADESEEERSGSQTESEEESSEMDDEDYERRRSECVSEMLDLEKQFSELKEKLFRERLSQLRLRLEEVGAERAPEYTEPLGGLQQSLKIRIQVAGIYKGFCLDVIRNKYECELQGAKQHLESEKMLLYDTLLGELQERIQRLEEDRQSLDISSEWWDDKLHSRSSSKAGDAMPPSKRKKAPLVSGPYIVYMLQEIDILEDWTAIKKARAAVSPQKRKADGP

Target ORF information:

RefSeq Version	NM_134155.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus breast cancer metastasis-suppressor 1 (Brms1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_134155.3

ORF Insert Sequence:

ATGCCCATCC AGCCTTCAGG CAAAGAAACA GAAGAGATGG AGGCAGAAGG GGATTCAGCA 
GCTGAGATGA ACGGGGAGGC AGATGAGAGC GAGGAGGAGC GGAGTGGCAG CCAAACTGAG 
TCAGAGGAGG AGAGCTCAGA GATGGATGAT GAAGACTATG AGCGGCGCCG CAGTGAGTGT 
GTCAGTGAGA TGCTGGACCT GGAAAAGCAG TTCTCAGAGC TGAAGGAGAA GTTGTTCAGG 
GAACGGCTCA GCCAGCTACG TTTACGACTG GAGGAAGTGG GGGCTGAGAG AGCCCCAGAA 
TATACGGAAC CTCTTGGCGG GCTGCAGCAG AGCCTGAAGA TCCGCATTCA GGTGGCAGGG 
ATCTACAAGG GCTTCTGTCT GGATGTGATC AGGAATAAGT ATGAGTGTGA ACTCCAGGGA 
GCCAAACAAC ACCTGGAGAG TGAGAAGATG CTGCTGTATG ACACACTGCT TGGGGAGCTG 
CAGGAGCGGA TCCAGAGGCT GGAGGAGGAC CGGCAGAGCC TTGACATCAG CTCTGAGTGG 
TGGGACGACA AACTACATAG CAGAAGCAGC TCCAAGGCCG GGGACGCCAT GCCACCCAGC 
AAGAGGAAGA AGGCACCGCT CGTTTCTGGT CCATACATTG TGTACATGCT GCAAGAGATC 
GACATCCTGG AAGACTGGAC AGCCATCAAA AAGGCCAGGG CAGCAGTGTC CCCTCAGAAG 
AGAAAAGCAG ACGGACCCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Brms1 ( NM_001374047.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001360976.1 Mus musculus breast cancer metastasis-suppressor 1 (Brms1), transcript variant 2, mRNA.

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CloneID	OMu10599
Clone ID Related Accession (Same CDS sequence)	NM_134155.1 , NM_134155.3 , NM_134155.2 , NM_001374047.1
Accession Version	NM_001374047.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 741bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-09
Organism	Mus musculus(house mouse)
Product	breast cancer metastasis-suppressor 1 homolog isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC124502.4. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: SRR7345562.3940897.1, SRR7345562.1927039.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END##

ATGCCCATCC AGCCTTCAGG CAAAGAAACA GAAGAGATGG AGGCAGAAGG GGATTCAGCA 
GCTGAGATGA ACGGGGAGGC AGATGAGAGC GAGGAGGAGC GGAGTGGCAG CCAAACTGAG 
TCAGAGGAGG AGAGCTCAGA GATGGATGAT GAAGACTATG AGCGGCGCCG CAGTGAGTGT 
GTCAGTGAGA TGCTGGACCT GGAAAAGCAG TTCTCAGAGC TGAAGGAGAA GTTGTTCAGG 
GAACGGCTCA GCCAGCTACG TTTACGACTG GAGGAAGTGG GGGCTGAGAG AGCCCCAGAA 
TATACGGAAC CTCTTGGCGG GCTGCAGCAG AGCCTGAAGA TCCGCATTCA GGTGGCAGGG 
ATCTACAAGG GCTTCTGTCT GGATGTGATC AGGAATAAGT ATGAGTGTGA ACTCCAGGGA 
GCCAAACAAC ACCTGGAGAG TGAGAAGATG CTGCTGTATG ACACACTGCT TGGGGAGCTG 
CAGGAGCGGA TCCAGAGGCT GGAGGAGGAC CGGCAGAGCC TTGACATCAG CTCTGAGTGG 
TGGGACGACA AACTACATAG CAGAAGCAGC TCCAAGGCCG GGGACGCCAT GCCACCCAGC 
AAGAGGAAGA AGGCACCGCT CGTTTCTGGT CCATACATTG TGTACATGCT GCAAGAGATC 
GACATCCTGG AAGACTGGAC AGCCATCAAA AAGGCCAGGG CAGCAGTGTC CCCTCAGAAG 
AGAAAAGCAG ACGGACCCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001360976.1
CDS	257..997
Translation	MPIQPSGKETEEMEAEGDSAAEMNGEADESEEERSGSQTESEEESSEMDDEDYERRRSECVSEMLDLEKQFSELKEKLFRERLSQLRLRLEEVGAERAPEYTEPLGGLQQSLKIRIQVAGIYKGFCLDVIRNKYECELQGAKQHLESEKMLLYDTLLGELQERIQRLEEDRQSLDISSEWWDDKLHSRSSSKAGDAMPPSKRKKAPLVSGPYIVYMLQEIDILEDWTAIKKARAAVSPQKRKADGP

Target ORF information:

RefSeq Version	NM_001374047.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus breast cancer metastasis-suppressor 1 (Brms1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001374047.1

ORF Insert Sequence:

ATGCCCATCC AGCCTTCAGG CAAAGAAACA GAAGAGATGG AGGCAGAAGG GGATTCAGCA 
GCTGAGATGA ACGGGGAGGC AGATGAGAGC GAGGAGGAGC GGAGTGGCAG CCAAACTGAG 
TCAGAGGAGG AGAGCTCAGA GATGGATGAT GAAGACTATG AGCGGCGCCG CAGTGAGTGT 
GTCAGTGAGA TGCTGGACCT GGAAAAGCAG TTCTCAGAGC TGAAGGAGAA GTTGTTCAGG 
GAACGGCTCA GCCAGCTACG TTTACGACTG GAGGAAGTGG GGGCTGAGAG AGCCCCAGAA 
TATACGGAAC CTCTTGGCGG GCTGCAGCAG AGCCTGAAGA TCCGCATTCA GGTGGCAGGG 
ATCTACAAGG GCTTCTGTCT GGATGTGATC AGGAATAAGT ATGAGTGTGA ACTCCAGGGA 
GCCAAACAAC ACCTGGAGAG TGAGAAGATG CTGCTGTATG ACACACTGCT TGGGGAGCTG 
CAGGAGCGGA TCCAGAGGCT GGAGGAGGAC CGGCAGAGCC TTGACATCAG CTCTGAGTGG 
TGGGACGACA AACTACATAG CAGAAGCAGC TCCAAGGCCG GGGACGCCAT GCCACCCAGC 
AAGAGGAAGA AGGCACCGCT CGTTTCTGGT CCATACATTG TGTACATGCT GCAAGAGATC 
GACATCCTGG AAGACTGGAC AGCCATCAAA AAGGCCAGGG CAGCAGTGTC CCCTCAGAAG 
AGAAAAGCAG ACGGACCCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Brms1 ( NM_001374048.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001360977.1 Mus musculus breast cancer metastasis-suppressor 1 (Brms1), transcript variant 3, mRNA.

Price & Availability↑

CloneID	OMu90564
Clone ID Related Accession (Same CDS sequence)	NM_001374048.1
Accession Version	NM_001374048.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 648bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-09
Organism	Mus musculus(house mouse)
Product	breast cancer metastasis-suppressor 1 homolog isoform 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC124502.4. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMN00849378, SAMN00849380 [ECO:0000348] ##Evidence-Data-END##

ATGCCCATCC AGCCTTCAGG CAAAGAAACA GAAGAGATGG AGGCAGAAGG GGATTCAGCA 
GCTGAGATGA ACGGGGAGGC AGATGAGAGC GAGGAGGAGC GGAGTGGCAG CCAAACTGAG 
TCAGAGGAGG AGAGCTCAGA GATGGATGAT GAAGACTATG AGCGGCGCCG CAGTGAGTGT 
GTCAGTGAGA TGCTGGACCT GGAAAAGCAG TTCTCAGAGC TGAAGGAGAA GTTGTTCAGG 
GAACGGCTCA GCCAGCTACG TTTACGACTG GAGGAAGTGG GGGCTGAGAG AGCCCCAGAA 
TATACGGAAC CTCTTGGCGG GCTGCAGCAG AGCCTGAAGA TCCGCATTCA GGTGGCAGGG 
ATCTACAAGG GCTTCTGTCT GGATGTGATC AGGAATAAGT ATGAGTGTGA ACTCCAGGGA 
GCCAAACAAC ACCTGGAGAG TGAGAAGATG CTGCTGTATG ACACACTGCT TGGGGAGCTG 
CAGGAGCGGA TCCAGAGGCT GGAGGAGGAC CGGCAGAGCC TTGACATCAG CTCTGGTCCA 
TACATTGTGT ACATGCTGCA AGAGATCGAC ATCCTGGAAG ACTGGACAGC CATCAAAAAG 
GCCAGGGCAG CAGTGTCCCC TCAGAAGAGA AAAGCAGACG GACCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001360977.1
CDS	136..783
Translation	MPIQPSGKETEEMEAEGDSAAEMNGEADESEEERSGSQTESEEESSEMDDEDYERRRSECVSEMLDLEKQFSELKEKLFRERLSQLRLRLEEVGAERAPEYTEPLGGLQQSLKIRIQVAGIYKGFCLDVIRNKYECELQGAKQHLESEKMLLYDTLLGELQERIQRLEEDRQSLDISSGPYIVYMLQEIDILEDWTAIKKARAAVSPQKRKADGP

Target ORF information:

RefSeq Version	NM_001374048.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus breast cancer metastasis-suppressor 1 (Brms1), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001374048.1

ORF Insert Sequence:

ATGCCCATCC AGCCTTCAGG CAAAGAAACA GAAGAGATGG AGGCAGAAGG GGATTCAGCA 
GCTGAGATGA ACGGGGAGGC AGATGAGAGC GAGGAGGAGC GGAGTGGCAG CCAAACTGAG 
TCAGAGGAGG AGAGCTCAGA GATGGATGAT GAAGACTATG AGCGGCGCCG CAGTGAGTGT 
GTCAGTGAGA TGCTGGACCT GGAAAAGCAG TTCTCAGAGC TGAAGGAGAA GTTGTTCAGG 
GAACGGCTCA GCCAGCTACG TTTACGACTG GAGGAAGTGG GGGCTGAGAG AGCCCCAGAA 
TATACGGAAC CTCTTGGCGG GCTGCAGCAG AGCCTGAAGA TCCGCATTCA GGTGGCAGGG 
ATCTACAAGG GCTTCTGTCT GGATGTGATC AGGAATAAGT ATGAGTGTGA ACTCCAGGGA 
GCCAAACAAC ACCTGGAGAG TGAGAAGATG CTGCTGTATG ACACACTGCT TGGGGAGCTG 
CAGGAGCGGA TCCAGAGGCT GGAGGAGGAC CGGCAGAGCC TTGACATCAG CTCTGGTCCA 
TACATTGTGT ACATGCTGCA AGAGATCGAC ATCCTGGAAG ACTGGACAGC CATCAAAAAG 
GCCAGGGCAG CAGTGTCCCC TCAGAAGAGA AAAGCAGACG GACCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

High-throughput discovery of novel developmental phenotypes.
Nature537(7621)508-514(2016 09)
Dickinson ME,Flenniken AM,Ji X,Teboul L,Wong MD,White JK,Meehan TF,Weninger WJ,Westerberg H,Adissu H,Baker CN,Bower L,Brown JM,Caddle LB,Chiani F,Clary D,Cleak J,Daly MJ,Denegre JM,Doe B,Dolan ME,Edie SM,Fuchs H,Gailus-Durner V,Galli A,Gambadoro A,Gallegos J,Guo S,Horner NR,Hsu CW,Johnson SJ,Kalaga S,Keith LC,Lanoue L,Lawson TN,Lek M,Mark M,Marschall S,Mason J,McElwee ML,Newbigging S,Nutter LM,Peterson KA,Ramirez-Solis R,Rowland DJ,Ryder E,Samocha KE,Seavitt JR,Selloum M,Szoke-Kovacs Z,Tamura M,Trainor AG,Tudose I,Wakana S,Warren J,Wendling O,West DB,Wong L,Yoshiki A,,,,,,,,,MacArthur DG,Tocchini-Valentini GP,Gao X,Flicek P,Bradley A,Skarnes WC,Justice MJ,Parkinson HE,Moore M,Wells S,Braun RE,Svenson KL,de Angelis MH,Herault Y,Mohun T,Mallon AM,Henkelman RM,Brown SD,Adams DJ,Lloyd KC,McKerlie C,Beaudet AL,Bu?an M,Murray SA

Ubiquitous Brms1 expression is critical for mammary carcinoma metastasis suppression via promotion of apoptosis.
Clinical & experimental metastasis29(4)315-25(2012 Apr)
Cook LM,Cao X,Dowell AE,Debies MT,Edmonds MD,Beck BH,Kesterson RA,Desmond RA,Frost AR,Hurst DR,Welch DR

Identification of essential sequences for cellular localization in BRMS1 metastasis suppressor.
PloS one4(7)e6433(2009 Jul)
Rivera J,Meg?as D,Navas C,Bravo J

Suppression of murine mammary carcinoma metastasis by the murine ortholog of breast cancer metastasis suppressor 1 (Brms1).
Cancer letters235(2)260-5(2006 Apr)
Samant RS,Debies MT,Hurst DR,Moore BP,Shevde LA,Welch DR

Breast cancer metastasis suppressor 1 (BRMS1) forms complexes with retinoblastoma-binding protein 1 (RBP1) and the mSin3 histone deacetylase complex and represses transcription.
The Journal of biological chemistry279(2)1562-9(2004 Jan)
Meehan WJ,Samant RS,Hopper JE,Carrozza MJ,Shevde LA,Workman JL,Eckert KA,Verderame MF,Welch DR

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Brms1 cDNA ORF clone, Mus musculus(house mouse)

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gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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