Trpt1 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Trpt1
Entrez Gene ID	107328
Full Name	tRNA phosphotransferase 1
Synonyms	AU079016,AW045591,Tpt1,Tpt1h
General protein information	Preferred Names tRNA phosphotransferase 1 Names tRNA 2'-phosphotransferase 1 EST-MNCb3719 mTPT1 tRNA splicing 2' phosphotransferase 1 homolog
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 19 Map Location: 19\|19 A

mRNA and Protein(s)

mRNA	Protein	Name
XM_006526577.2	XP_006526640.1	tRNA 2'-phosphotransferase 1 isoform X1
XM_006526579.2	XP_006526642.1	tRNA 2'-phosphotransferase 1 isoform X3
XM_006526577.4	XP_006526640.1	tRNA 2'-phosphotransferase 1 isoform X1
XM_006526578.4	XP_006526641.1	tRNA 2'-phosphotransferase 1 isoform X2
XM_006526579.3	XP_006526642.1	tRNA 2'-phosphotransferase 1 isoform X3
NM_153597.2	NP_705825.2	tRNA 2'-phosphotransferase 1
XM_006526577.3	XP_006526640.1	tRNA 2'-phosphotransferase 1 isoform X1
XM_006526578.3	XP_006526641.1	tRNA 2'-phosphotransferase 1 isoform X2

Pathways from BioSystems

Homologies

Rattus norvegicus (Norway rat)	Trpt1	NP_001099801.1
Drosophila melanogaster (fruit fly)	CG33057	NP_788477.1
Schizosaccharomyces pombe (fission yeast)	SPAC2C4.12c	NP_594515.2
Arabidopsis thaliana (thale cress)	emb1067	NP_182058.2
Homo sapiens (human)	TRPT1	NP_001153861.1
Danio rerio (zebrafish)	trpt1	NP_001013309.2
Arabidopsis thaliana (thale cress)	AT5G23600	NP_197750.1
Bos taurus (cattle)	TRPT1	NP_001160074.1
Xenopus tropicalis (tropical clawed frog)	trpt1	XP_002940273.1
Pan troglodytes (chimpanzee)	TRPT1	XP_508523.4
	LOC717911	XP_001115358.2
Canis lupus familiaris (dog)	TRPT1	XP_540886.4
Mus musculus (house mouse)	Trpt1	NP_705825.2
Oryza sativa (rice)	Os06g0134050	NP_001174584.1

Gene Ontology
Bibliography

Related articles in PubMed

An intact unfolded protein response in Trpt1 knockout mice reveals phylogenic divergence in pathways for RNA ligation.
Harding HP, Lackey JG, Hsu HC, Zhang Y, Deng J, Xu RM, Damha MJ, Ron D
RNA (New York, N.Y.)14(2)225-32(2008 Feb)

SAGE2Splice: unmapped SAGE tags reveal novel splice junctions.
Kuo BY, Chen Y, Bohacec S, Johansson O, Wasserman WW, Simpson EM
PLoS computational biology2(4)e34(2006 Apr)

Spermatogonia-dependent expression of testicular genes in mice.
Tanaka K, Tamura H, Tanaka H, Katoh M, Futamata Y, Seki N, Nishimune Y, Hara T
Developmental biology246(2)466-79(2002 Jun)

A functional homolog of a yeast tRNA splicing enzyme is conserved in higher eukaryotes and in Escherichia coli.
Spinelli SL, Malik HS, Consaul SA, Phizicky EM
Proceedings of the National Academy of Sciences of the United States of America95(24)14136-41(1998 Nov)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Inactivation of the Trpt1 gene, encoding the only known mammalian homolog of Tpt1p, eliminates all detectable 2'-phosphotransferase activity from cultured mouse cells but has no measurable effect on spliced Xbp-1 translation.
Title: An intact unfolded protein response in Trpt1 knockout mice reveals phylogenic divergence in pathways for RNA ligation.

The following Trpt1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Trpt1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu02957	NM_153597.2 Latest version!	Mus musculus tRNA phosphotransferase 1 (Trpt1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$139.30 $199.00
OMu02957	XM_006526577.4 Latest version!	Mus musculus tRNA phosphotransferase 1 (Trpt1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$139.30 $199.00
OMu55175	XM_006526578.4 Latest version!	Mus musculus tRNA phosphotransferase 1 (Trpt1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$139.30 $199.00
OMu55176	XM_006526579.3 Latest version!	Mus musculus tRNA phosphotransferase 1 (Trpt1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$69.30 $99.00
View Old Accession Versions >>
OMu02957	XM_006526577.2	Mus musculus tRNA phosphotransferase 1 (Trpt1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$139.30 $199.00
OMu55176	XM_006526579.2	Mus musculus tRNA phosphotransferase 1 (Trpt1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00
OMu02957	XM_006526577.3	Mus musculus tRNA phosphotransferase 1 (Trpt1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$139.30 $199.00
OMu55175	XM_006526578.3	Mus musculus tRNA phosphotransferase 1 (Trpt1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$139.30 $199.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Trpt1 ( XM_006526578.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006526641.1 Mus musculus tRNA phosphotransferase 1 (Trpt1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OMu55175
Clone ID Related Accession (Same CDS sequence)	XM_006526578.4 , XM_006526578.3
Accession Version	XM_006526578.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 702bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	tRNA 2'-phosphotransferase 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000085.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006526578.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAACGCCC CTGGAGGAAG GAGGAAGGAA GGTAGAAGGA CTCATAGACC CCGGGAACAG 
GACCGAAATG TGCAGCTGTC TAAGGCTCTG TCCTATGCCC TTCGCCACGG GGCCTTGAAG 
CTGGGACTTC CCATGCGAGC TGATGGCTTT GTGCCCCTGC AAGCCCTCCT GCAGCTGCCC 
CAGTTCCACA GCTTTTCCAT TGAAGATGTG CAGCTCGTGG TCAATACCAA CGAAAAGCAG 
CGGTTTACCC TGCAGCCAGG CGAGCCGAGC ACTGGGCTCC TCATCAGGGC CAATCAGGGC 
CACTCCCTAC AAGTCCCTGA GTTGGAACTG ACGCCCCTGG AGACACCACA AGCCCTGCCC 
TTGACACTAG TCCATGGTAC ATTCTGGAAG CACTGGCCAT CTATTCTACT AAAGGGCTTG 
TCACGCCAAG GAAGGACACA CATCCACTTG GCCTCAGGAT TGCCTGGGGA CCCTGGTGTC 
ATCAGCGGCA TTCGTCCAAA TTGTGAGGTG GCGGTGTTCA TCGATGGACC CCTAGCCCTC 
ACAGGGAATG CTGAAGGCTT CCTGCTTCCC AAGTACTTCA AGGAGGCCCT GCAGCTTCGA 
CCTACACGAA AGCCTCTCTC CTTGGCTGGT GATAAAGAGA CAGAGACTCA GAGTGGCCCC 
AAGCTCAGTT CCAGAGGAGG AAGAAGAAAG ATCCAACAAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006526641.1
CDS	323..1024
Translation	MNAPGGRRKEGRRTHRPREQDRNVQLSKALSYALRHGALKLGLPMRADGFVPLQALLQLPQFHSFSIEDVQLVVNTNEKQRFTLQPGEPSTGLLIRANQGHSLQVPELELTPLETPQALPLTLVHGTFWKHWPSILLKGLSRQGRTHIHLASGLPGDPGVISGIRPNCEVAVFIDGPLALTGNAEGFLLPKYFKEALQLRPTRKPLSLAGDKETETQSGPKLSSRGGRRKIQQ

Target ORF information:

RefSeq Version	XM_006526578.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus tRNA phosphotransferase 1 (Trpt1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006526578.3

ORF Insert Sequence:

ATGAACGCCC CTGGAGGAAG GAGGAAGGAA GGTAGAAGGA CTCATAGACC CCGGGAACAG 
GACCGAAATG TGCAGCTGTC TAAGGCTCTG TCCTATGCCC TTCGCCACGG GGCCTTGAAG 
CTGGGACTTC CCATGCGAGC TGATGGCTTT GTGCCCCTGC AAGCCCTCCT GCAGCTGCCC 
CAGTTCCACA GCTTTTCCAT TGAAGATGTG CAGCTCGTGG TCAATACCAA CGAAAAGCAG 
CGGTTTACCC TGCAGCCAGG CGAGCCGAGC ACTGGGCTCC TCATCAGGGC CAATCAGGGC 
CACTCCCTAC AAGTCCCTGA GTTGGAACTG ACGCCCCTGG AGACACCACA AGCCCTGCCC 
TTGACACTAG TCCATGGTAC ATTCTGGAAG CACTGGCCAT CTATTCTACT AAAGGGCTTG 
TCACGCCAAG GAAGGACACA CATCCACTTG GCCTCAGGAT TGCCTGGGGA CCCTGGTGTC 
ATCAGCGGCA TTCGTCCAAA TTGTGAGGTG GCGGTGTTCA TCGATGGACC CCTAGCCCTC 
ACAGGGAATG CTGAAGGCTT CCTGCTTCCC AAGTACTTCA AGGAGGCCCT GCAGCTTCGA 
CCTACACGAA AGCCTCTCTC CTTGGCTGGT GATAAAGAGA CAGAGACTCA GAGTGGCCCC 
AAGCTCAGTT CCAGAGGAGG AAGAAGAAAG ATCCAACAAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Trpt1 ( XM_006526579.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006526642.1 Mus musculus tRNA phosphotransferase 1 (Trpt1), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OMu55176
Clone ID Related Accession (Same CDS sequence)	XM_006526579.2 , XM_006526579.3
Accession Version	XM_006526579.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 405bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	tRNA 2'-phosphotransferase 1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000085.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Feb 9, 2015 this sequence version replaced XM_006526579.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAACGCCC CTGGAGGAAG GAGGAAGGAA GGTAGAAGGA CTCATAGACC CCGGGAACAG 
GACCGAAATG TGCAGCTGTC TAAGGCTCTG TCCTATGCCC TTCGCCACGG GGCCTTGAAG 
CTGGGACTTC CCATGCGAGC TGGCATTCGT CCAAATTGTG AGGTGGCGGT GTTCATCGAT 
GGACCCCTAG CCCTCACAGA TGGAATCCCT TTCTTCTGCT CCGCCAATGG TGTGATTCTG 
ACTCCAGGGA ATGCTGAAGG CTTCCTGCTT CCCAAGTACT TCAAGGAGGC CCTGCAGCTT 
CGACCTACAC GAAAGCCTCT CTCCTTGGCT GGTGATAAAG AGACAGAGAC TCAGAGTGGC 
CCCAAGCTCA GTTCCAGAGG AGGAAGAAGA AAGATCCAAC AATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006526642.1
CDS	328..732
Translation	MNAPGGRRKEGRRTHRPREQDRNVQLSKALSYALRHGALKLGLPMRAGIRPNCEVAVFIDGPLALTDGIPFFCSANGVILTPGNAEGFLLPKYFKEALQLRPTRKPLSLAGDKETETQSGPKLSSRGGRRKIQQ

Target ORF information:

RefSeq Version	XM_006526579.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus tRNA phosphotransferase 1 (Trpt1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006526579.2

ORF Insert Sequence:

ATGAACGCCC CTGGAGGAAG GAGGAAGGAA GGTAGAAGGA CTCATAGACC CCGGGAACAG 
GACCGAAATG TGCAGCTGTC TAAGGCTCTG TCCTATGCCC TTCGCCACGG GGCCTTGAAG 
CTGGGACTTC CCATGCGAGC TGGCATTCGT CCAAATTGTG AGGTGGCGGT GTTCATCGAT 
GGACCCCTAG CCCTCACAGA TGGAATCCCT TTCTTCTGCT CCGCCAATGG TGTGATTCTG 
ACTCCAGGGA ATGCTGAAGG CTTCCTGCTT CCCAAGTACT TCAAGGAGGC CCTGCAGCTT 
CGACCTACAC GAAAGCCTCT CTCCTTGGCT GGTGATAAAG AGACAGAGAC TCAGAGTGGC 
CCCAAGCTCA GTTCCAGAGG AGGAAGAAGA AAGATCCAAC AATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Trpt1 ( NM_153597.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_705825.2 Mus musculus tRNA phosphotransferase 1 (Trpt1), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OMu02957
Clone ID Related Accession (Same CDS sequence)	XM_006526577.2 , XM_006526577.4 , NM_153597.2 , XM_006526577.3
Accession Version	NM_153597.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 750bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-19
Organism	Mus musculus(house mouse)
Product	tRNA 2'-phosphotransferase 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK038830.1 and BC027575.1. On Mar 14, 2009 this sequence version replaced NM_153597.1. Transcript Variant: This variant (1) represents the longer transcript and is protein-coding. ##Evidence-Data-START## Transcript exon combination :: BC027575.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAACGCCC CTGGAGGAAG GAGGAAGGAA GGTAGAAGGA CTCATAGACC CCGGGAACAG 
GACCGAAATG TGCAGCTGTC TAAGGCTCTG TCCTATGCCC TTCGCCACGG GGCCTTGAAG 
CTGGGACTTC CCATGCGAGC TGATGGCTTT GTGCCCCTGC AAGCCCTCCT GCAGCTGCCC 
CAGTTCCACA GCTTTTCCAT TGAAGATGTG CAGCTCGTGG TCAATACCAA CGAAAAGCAG 
CGGTTTACCC TGCAGCCAGG CGAGCCGAGC ACTGGGCTCC TCATCAGGGC CAATCAGGGC 
CACTCCCTAC AAGTCCCTGA GTTGGAACTG ACGCCCCTGG AGACACCACA AGCCCTGCCC 
TTGACACTAG TCCATGGTAC ATTCTGGAAG CACTGGCCAT CTATTCTACT AAAGGGCTTG 
TCACGCCAAG GAAGGACACA CATCCACTTG GCCTCAGGAT TGCCTGGGGA CCCTGGTGTC 
ATCAGCGGCA TTCGTCCAAA TTGTGAGGTG GCGGTGTTCA TCGATGGACC CCTAGCCCTC 
ACAGATGGAA TCCCTTTCTT CTGCTCCGCC AATGGTGTGA TTCTGACTCC AGGGAATGCT 
GAAGGCTTCC TGCTTCCCAA GTACTTCAAG GAGGCCCTGC AGCTTCGACC TACACGAAAG 
CCTCTCTCCT TGGCTGGTGA TAAAGAGACA GAGACTCAGA GTGGCCCCAA GCTCAGTTCC 
AGAGGAGGAA GAAGAAAGAT CCAACAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_705825.2
CDS	335..1084
Translation	MNAPGGRRKEGRRTHRPREQDRNVQLSKALSYALRHGALKLGLPMRADGFVPLQALLQLPQFHSFSIEDVQLVVNTNEKQRFTLQPGEPSTGLLIRANQGHSLQVPELELTPLETPQALPLTLVHGTFWKHWPSILLKGLSRQGRTHIHLASGLPGDPGVISGIRPNCEVAVFIDGPLALTDGIPFFCSANGVILTPGNAEGFLLPKYFKEALQLRPTRKPLSLAGDKETETQSGPKLSSRGGRRKIQQ

Target ORF information:

RefSeq Version	NM_153597.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus tRNA phosphotransferase 1 (Trpt1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_153597.2

ORF Insert Sequence:

ATGAACGCCC CTGGAGGAAG GAGGAAGGAA GGTAGAAGGA CTCATAGACC CCGGGAACAG 
GACCGAAATG TGCAGCTGTC TAAGGCTCTG TCCTATGCCC TTCGCCACGG GGCCTTGAAG 
CTGGGACTTC CCATGCGAGC TGATGGCTTT GTGCCCCTGC AAGCCCTCCT GCAGCTGCCC 
CAGTTCCACA GCTTTTCCAT TGAAGATGTG CAGCTCGTGG TCAATACCAA CGAAAAGCAG 
CGGTTTACCC TGCAGCCAGG CGAGCCGAGC ACTGGGCTCC TCATCAGGGC CAATCAGGGC 
CACTCCCTAC AAGTCCCTGA GTTGGAACTG ACGCCCCTGG AGACACCACA AGCCCTGCCC 
TTGACACTAG TCCATGGTAC ATTCTGGAAG CACTGGCCAT CTATTCTACT AAAGGGCTTG 
TCACGCCAAG GAAGGACACA CATCCACTTG GCCTCAGGAT TGCCTGGGGA CCCTGGTGTC 
ATCAGCGGCA TTCGTCCAAA TTGTGAGGTG GCGGTGTTCA TCGATGGACC CCTAGCCCTC 
ACAGATGGAA TCCCTTTCTT CTGCTCCGCC AATGGTGTGA TTCTGACTCC AGGGAATGCT 
GAAGGCTTCC TGCTTCCCAA GTACTTCAAG GAGGCCCTGC AGCTTCGACC TACACGAAAG 
CCTCTCTCCT TGGCTGGTGA TAAAGAGACA GAGACTCAGA GTGGCCCCAA GCTCAGTTCC 
AGAGGAGGAA GAAGAAAGAT CCAACAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Trpt1 ( XM_006526577.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006526640.1 Mus musculus tRNA phosphotransferase 1 (Trpt1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu02957
Clone ID Related Accession (Same CDS sequence)	XM_006526577.2 , XM_006526577.4 , NM_153597.2 , XM_006526577.3
Accession Version	XM_006526577.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 750bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-02-08
Organism	Mus musculus(House mouse)
Product	tRNA 2'-phosphotransferase 1 isoform X1
Comment

ATGAACGCCC CTGGAGGAAG GAGGAAGGAA GGTAGAAGGA CTCATAGACC CCGGGAACAG 
GACCGAAATG TGCAGCTGTC TAAGGCTCTG TCCTATGCCC TTCGCCACGG GGCCTTGAAG 
CTGGGACTTC CCATGCGAGC TGATGGCTTT GTGCCCCTGC AAGCCCTCCT GCAGCTGCCC 
CAGTTCCACA GCTTTTCCAT TGAAGATGTG CAGCTCGTGG TCAATACCAA CGAAAAGCAG 
CGGTTTACCC TGCAGCCAGG CGAGCCGAGC ACTGGGCTCC TCATCAGGGC CAATCAGGGC 
CACTCCCTAC AAGTCCCTGA GTTGGAACTG ACGCCCCTGG AGACACCACA AGCCCTGCCC 
TTGACACTAG TCCATGGTAC ATTCTGGAAG CACTGGCCAT CTATTCTACT AAAGGGCTTG 
TCACGCCAAG GAAGGACACA CATCCACTTG GCCTCAGGAT TGCCTGGGGA CCCTGGTGTC 
ATCAGCGGCA TTCGTCCAAA TTGTGAGGTG GCGGTGTTCA TCGATGGACC CCTAGCCCTC 
ACAGATGGAA TCCCTTTCTT CTGCTCCGCC AATGGTGTGA TTCTGACTCC AGGGAATGCT 
GAAGGCTTCC TGCTTCCCAA GTACTTCAAG GAGGCCCTGC AGCTTCGACC TACACGAAAG 
CCTCTCTCCT TGGCTGGTGA TAAAGAGACA GAGACTCAGA GTGGCCCCAA GCTCAGTTCC 
AGAGGAGGAA GAAGAAAGAT CCAACAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006526640.1
Translation	MNAPGGRRKEGRRTHRPREQDRNVQLSKALSYALRHGALKLGLPMRADGFVPLQALLQLPQFHSFSIEDVQLVVNTNEKQRFTLQPGEPSTGLLIRANQGHSLQVPELELTPLETPQALPLTLVHGTFWKHWPSILLKGLSRQGRTHIHLASGLPGDPGVISGIRPNCEVAVFIDGPLALTDGIPFFCSANGVILTPGNAEGFLLPKYFKEALQLRPTRKPLSLAGDKETETQSGPKLSSRGGRRKIQQ

Target ORF information:

RefSeq Version	XM_006526577.2
Organism	Mus musculus(House mouse)
Definition	Mus musculus tRNA phosphotransferase 1 (Trpt1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006526577.2

ORF Insert Sequence:

ATGAACGCCC CTGGAGGAAG GAGGAAGGAA GGTAGAAGGA CTCATAGACC CCGGGAACAG 
GACCGAAATG TGCAGCTGTC TAAGGCTCTG TCCTATGCCC TTCGCCACGG GGCCTTGAAG 
CTGGGACTTC CCATGCGAGC TGATGGCTTT GTGCCCCTGC AAGCCCTCCT GCAGCTGCCC 
CAGTTCCACA GCTTTTCCAT TGAAGATGTG CAGCTCGTGG TCAATACCAA CGAAAAGCAG 
CGGTTTACCC TGCAGCCAGG CGAGCCGAGC ACTGGGCTCC TCATCAGGGC CAATCAGGGC 
CACTCCCTAC AAGTCCCTGA GTTGGAACTG ACGCCCCTGG AGACACCACA AGCCCTGCCC 
TTGACACTAG TCCATGGTAC ATTCTGGAAG CACTGGCCAT CTATTCTACT AAAGGGCTTG 
TCACGCCAAG GAAGGACACA CATCCACTTG GCCTCAGGAT TGCCTGGGGA CCCTGGTGTC 
ATCAGCGGCA TTCGTCCAAA TTGTGAGGTG GCGGTGTTCA TCGATGGACC CCTAGCCCTC 
ACAGATGGAA TCCCTTTCTT CTGCTCCGCC AATGGTGTGA TTCTGACTCC AGGGAATGCT 
GAAGGCTTCC TGCTTCCCAA GTACTTCAAG GAGGCCCTGC AGCTTCGACC TACACGAAAG 
CCTCTCTCCT TGGCTGGTGA TAAAGAGACA GAGACTCAGA GTGGCCCCAA GCTCAGTTCC 
AGAGGAGGAA GAAGAAAGAT CCAACAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Trpt1 ( XM_006526577.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006526640.1 Mus musculus tRNA phosphotransferase 1 (Trpt1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu02957
Clone ID Related Accession (Same CDS sequence)	XM_006526577.2 , XM_006526577.4 , NM_153597.2 , XM_006526577.3
Accession Version	XM_006526577.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 750bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	tRNA 2'-phosphotransferase 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000085.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006526577.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAACGCCC CTGGAGGAAG GAGGAAGGAA GGTAGAAGGA CTCATAGACC CCGGGAACAG 
GACCGAAATG TGCAGCTGTC TAAGGCTCTG TCCTATGCCC TTCGCCACGG GGCCTTGAAG 
CTGGGACTTC CCATGCGAGC TGATGGCTTT GTGCCCCTGC AAGCCCTCCT GCAGCTGCCC 
CAGTTCCACA GCTTTTCCAT TGAAGATGTG CAGCTCGTGG TCAATACCAA CGAAAAGCAG 
CGGTTTACCC TGCAGCCAGG CGAGCCGAGC ACTGGGCTCC TCATCAGGGC CAATCAGGGC 
CACTCCCTAC AAGTCCCTGA GTTGGAACTG ACGCCCCTGG AGACACCACA AGCCCTGCCC 
TTGACACTAG TCCATGGTAC ATTCTGGAAG CACTGGCCAT CTATTCTACT AAAGGGCTTG 
TCACGCCAAG GAAGGACACA CATCCACTTG GCCTCAGGAT TGCCTGGGGA CCCTGGTGTC 
ATCAGCGGCA TTCGTCCAAA TTGTGAGGTG GCGGTGTTCA TCGATGGACC CCTAGCCCTC 
ACAGATGGAA TCCCTTTCTT CTGCTCCGCC AATGGTGTGA TTCTGACTCC AGGGAATGCT 
GAAGGCTTCC TGCTTCCCAA GTACTTCAAG GAGGCCCTGC AGCTTCGACC TACACGAAAG 
CCTCTCTCCT TGGCTGGTGA TAAAGAGACA GAGACTCAGA GTGGCCCCAA GCTCAGTTCC 
AGAGGAGGAA GAAGAAAGAT CCAACAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006526640.1
CDS	101..850
Translation	MNAPGGRRKEGRRTHRPREQDRNVQLSKALSYALRHGALKLGLPMRADGFVPLQALLQLPQFHSFSIEDVQLVVNTNEKQRFTLQPGEPSTGLLIRANQGHSLQVPELELTPLETPQALPLTLVHGTFWKHWPSILLKGLSRQGRTHIHLASGLPGDPGVISGIRPNCEVAVFIDGPLALTDGIPFFCSANGVILTPGNAEGFLLPKYFKEALQLRPTRKPLSLAGDKETETQSGPKLSSRGGRRKIQQ

Target ORF information:

RefSeq Version	XM_006526577.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus tRNA phosphotransferase 1 (Trpt1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006526577.4

ORF Insert Sequence:

ATGAACGCCC CTGGAGGAAG GAGGAAGGAA GGTAGAAGGA CTCATAGACC CCGGGAACAG 
GACCGAAATG TGCAGCTGTC TAAGGCTCTG TCCTATGCCC TTCGCCACGG GGCCTTGAAG 
CTGGGACTTC CCATGCGAGC TGATGGCTTT GTGCCCCTGC AAGCCCTCCT GCAGCTGCCC 
CAGTTCCACA GCTTTTCCAT TGAAGATGTG CAGCTCGTGG TCAATACCAA CGAAAAGCAG 
CGGTTTACCC TGCAGCCAGG CGAGCCGAGC ACTGGGCTCC TCATCAGGGC CAATCAGGGC 
CACTCCCTAC AAGTCCCTGA GTTGGAACTG ACGCCCCTGG AGACACCACA AGCCCTGCCC 
TTGACACTAG TCCATGGTAC ATTCTGGAAG CACTGGCCAT CTATTCTACT AAAGGGCTTG 
TCACGCCAAG GAAGGACACA CATCCACTTG GCCTCAGGAT TGCCTGGGGA CCCTGGTGTC 
ATCAGCGGCA TTCGTCCAAA TTGTGAGGTG GCGGTGTTCA TCGATGGACC CCTAGCCCTC 
ACAGATGGAA TCCCTTTCTT CTGCTCCGCC AATGGTGTGA TTCTGACTCC AGGGAATGCT 
GAAGGCTTCC TGCTTCCCAA GTACTTCAAG GAGGCCCTGC AGCTTCGACC TACACGAAAG 
CCTCTCTCCT TGGCTGGTGA TAAAGAGACA GAGACTCAGA GTGGCCCCAA GCTCAGTTCC 
AGAGGAGGAA GAAGAAAGAT CCAACAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Trpt1 ( XM_006526577.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006526640.1 Mus musculus tRNA phosphotransferase 1 (Trpt1), transcript variant X1, mRNA.

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CloneID	OMu02957
Clone ID Related Accession (Same CDS sequence)	XM_006526577.2 , XM_006526577.4 , NM_153597.2 , XM_006526577.3
Accession Version	XM_006526577.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 750bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	tRNA 2'-phosphotransferase 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000085.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006526577.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAACGCCC CTGGAGGAAG GAGGAAGGAA GGTAGAAGGA CTCATAGACC CCGGGAACAG 
GACCGAAATG TGCAGCTGTC TAAGGCTCTG TCCTATGCCC TTCGCCACGG GGCCTTGAAG 
CTGGGACTTC CCATGCGAGC TGATGGCTTT GTGCCCCTGC AAGCCCTCCT GCAGCTGCCC 
CAGTTCCACA GCTTTTCCAT TGAAGATGTG CAGCTCGTGG TCAATACCAA CGAAAAGCAG 
CGGTTTACCC TGCAGCCAGG CGAGCCGAGC ACTGGGCTCC TCATCAGGGC CAATCAGGGC 
CACTCCCTAC AAGTCCCTGA GTTGGAACTG ACGCCCCTGG AGACACCACA AGCCCTGCCC 
TTGACACTAG TCCATGGTAC ATTCTGGAAG CACTGGCCAT CTATTCTACT AAAGGGCTTG 
TCACGCCAAG GAAGGACACA CATCCACTTG GCCTCAGGAT TGCCTGGGGA CCCTGGTGTC 
ATCAGCGGCA TTCGTCCAAA TTGTGAGGTG GCGGTGTTCA TCGATGGACC CCTAGCCCTC 
ACAGATGGAA TCCCTTTCTT CTGCTCCGCC AATGGTGTGA TTCTGACTCC AGGGAATGCT 
GAAGGCTTCC TGCTTCCCAA GTACTTCAAG GAGGCCCTGC AGCTTCGACC TACACGAAAG 
CCTCTCTCCT TGGCTGGTGA TAAAGAGACA GAGACTCAGA GTGGCCCCAA GCTCAGTTCC 
AGAGGAGGAA GAAGAAAGAT CCAACAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006526640.1
CDS	243..992
Translation	MNAPGGRRKEGRRTHRPREQDRNVQLSKALSYALRHGALKLGLPMRADGFVPLQALLQLPQFHSFSIEDVQLVVNTNEKQRFTLQPGEPSTGLLIRANQGHSLQVPELELTPLETPQALPLTLVHGTFWKHWPSILLKGLSRQGRTHIHLASGLPGDPGVISGIRPNCEVAVFIDGPLALTDGIPFFCSANGVILTPGNAEGFLLPKYFKEALQLRPTRKPLSLAGDKETETQSGPKLSSRGGRRKIQQ

Target ORF information:

RefSeq Version	XM_006526577.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus tRNA phosphotransferase 1 (Trpt1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006526577.3

ORF Insert Sequence:

ATGAACGCCC CTGGAGGAAG GAGGAAGGAA GGTAGAAGGA CTCATAGACC CCGGGAACAG 
GACCGAAATG TGCAGCTGTC TAAGGCTCTG TCCTATGCCC TTCGCCACGG GGCCTTGAAG 
CTGGGACTTC CCATGCGAGC TGATGGCTTT GTGCCCCTGC AAGCCCTCCT GCAGCTGCCC 
CAGTTCCACA GCTTTTCCAT TGAAGATGTG CAGCTCGTGG TCAATACCAA CGAAAAGCAG 
CGGTTTACCC TGCAGCCAGG CGAGCCGAGC ACTGGGCTCC TCATCAGGGC CAATCAGGGC 
CACTCCCTAC AAGTCCCTGA GTTGGAACTG ACGCCCCTGG AGACACCACA AGCCCTGCCC 
TTGACACTAG TCCATGGTAC ATTCTGGAAG CACTGGCCAT CTATTCTACT AAAGGGCTTG 
TCACGCCAAG GAAGGACACA CATCCACTTG GCCTCAGGAT TGCCTGGGGA CCCTGGTGTC 
ATCAGCGGCA TTCGTCCAAA TTGTGAGGTG GCGGTGTTCA TCGATGGACC CCTAGCCCTC 
ACAGATGGAA TCCCTTTCTT CTGCTCCGCC AATGGTGTGA TTCTGACTCC AGGGAATGCT 
GAAGGCTTCC TGCTTCCCAA GTACTTCAAG GAGGCCCTGC AGCTTCGACC TACACGAAAG 
CCTCTCTCCT TGGCTGGTGA TAAAGAGACA GAGACTCAGA GTGGCCCCAA GCTCAGTTCC 
AGAGGAGGAA GAAGAAAGAT CCAACAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Trpt1 ( XM_006526578.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006526641.1 Mus musculus tRNA phosphotransferase 1 (Trpt1), transcript variant X2, mRNA.

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CloneID	OMu55175
Clone ID Related Accession (Same CDS sequence)	XM_006526578.4 , XM_006526578.3
Accession Version	XM_006526578.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 702bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	tRNA 2'-phosphotransferase 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000085.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006526578.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAACGCCC CTGGAGGAAG GAGGAAGGAA GGTAGAAGGA CTCATAGACC CCGGGAACAG 
GACCGAAATG TGCAGCTGTC TAAGGCTCTG TCCTATGCCC TTCGCCACGG GGCCTTGAAG 
CTGGGACTTC CCATGCGAGC TGATGGCTTT GTGCCCCTGC AAGCCCTCCT GCAGCTGCCC 
CAGTTCCACA GCTTTTCCAT TGAAGATGTG CAGCTCGTGG TCAATACCAA CGAAAAGCAG 
CGGTTTACCC TGCAGCCAGG CGAGCCGAGC ACTGGGCTCC TCATCAGGGC CAATCAGGGC 
CACTCCCTAC AAGTCCCTGA GTTGGAACTG ACGCCCCTGG AGACACCACA AGCCCTGCCC 
TTGACACTAG TCCATGGTAC ATTCTGGAAG CACTGGCCAT CTATTCTACT AAAGGGCTTG 
TCACGCCAAG GAAGGACACA CATCCACTTG GCCTCAGGAT TGCCTGGGGA CCCTGGTGTC 
ATCAGCGGCA TTCGTCCAAA TTGTGAGGTG GCGGTGTTCA TCGATGGACC CCTAGCCCTC 
ACAGGGAATG CTGAAGGCTT CCTGCTTCCC AAGTACTTCA AGGAGGCCCT GCAGCTTCGA 
CCTACACGAA AGCCTCTCTC CTTGGCTGGT GATAAAGAGA CAGAGACTCA GAGTGGCCCC 
AAGCTCAGTT CCAGAGGAGG AAGAAGAAAG ATCCAACAAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006526641.1
CDS	356..1057
Translation	MNAPGGRRKEGRRTHRPREQDRNVQLSKALSYALRHGALKLGLPMRADGFVPLQALLQLPQFHSFSIEDVQLVVNTNEKQRFTLQPGEPSTGLLIRANQGHSLQVPELELTPLETPQALPLTLVHGTFWKHWPSILLKGLSRQGRTHIHLASGLPGDPGVISGIRPNCEVAVFIDGPLALTGNAEGFLLPKYFKEALQLRPTRKPLSLAGDKETETQSGPKLSSRGGRRKIQQ

Target ORF information:

RefSeq Version	XM_006526578.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus tRNA phosphotransferase 1 (Trpt1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006526578.4

ORF Insert Sequence:

ATGAACGCCC CTGGAGGAAG GAGGAAGGAA GGTAGAAGGA CTCATAGACC CCGGGAACAG 
GACCGAAATG TGCAGCTGTC TAAGGCTCTG TCCTATGCCC TTCGCCACGG GGCCTTGAAG 
CTGGGACTTC CCATGCGAGC TGATGGCTTT GTGCCCCTGC AAGCCCTCCT GCAGCTGCCC 
CAGTTCCACA GCTTTTCCAT TGAAGATGTG CAGCTCGTGG TCAATACCAA CGAAAAGCAG 
CGGTTTACCC TGCAGCCAGG CGAGCCGAGC ACTGGGCTCC TCATCAGGGC CAATCAGGGC 
CACTCCCTAC AAGTCCCTGA GTTGGAACTG ACGCCCCTGG AGACACCACA AGCCCTGCCC 
TTGACACTAG TCCATGGTAC ATTCTGGAAG CACTGGCCAT CTATTCTACT AAAGGGCTTG 
TCACGCCAAG GAAGGACACA CATCCACTTG GCCTCAGGAT TGCCTGGGGA CCCTGGTGTC 
ATCAGCGGCA TTCGTCCAAA TTGTGAGGTG GCGGTGTTCA TCGATGGACC CCTAGCCCTC 
ACAGGGAATG CTGAAGGCTT CCTGCTTCCC AAGTACTTCA AGGAGGCCCT GCAGCTTCGA 
CCTACACGAA AGCCTCTCTC CTTGGCTGGT GATAAAGAGA CAGAGACTCA GAGTGGCCCC 
AAGCTCAGTT CCAGAGGAGG AAGAAGAAAG ATCCAACAAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Trpt1 ( XM_006526579.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006526642.1 Mus musculus tRNA phosphotransferase 1 (Trpt1), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OMu55176
Clone ID Related Accession (Same CDS sequence)	XM_006526579.2 , XM_006526579.3
Accession Version	XM_006526579.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 405bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	tRNA 2'-phosphotransferase 1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000085.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006526579.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAACGCCC CTGGAGGAAG GAGGAAGGAA GGTAGAAGGA CTCATAGACC CCGGGAACAG 
GACCGAAATG TGCAGCTGTC TAAGGCTCTG TCCTATGCCC TTCGCCACGG GGCCTTGAAG 
CTGGGACTTC CCATGCGAGC TGGCATTCGT CCAAATTGTG AGGTGGCGGT GTTCATCGAT 
GGACCCCTAG CCCTCACAGA TGGAATCCCT TTCTTCTGCT CCGCCAATGG TGTGATTCTG 
ACTCCAGGGA ATGCTGAAGG CTTCCTGCTT CCCAAGTACT TCAAGGAGGC CCTGCAGCTT 
CGACCTACAC GAAAGCCTCT CTCCTTGGCT GGTGATAAAG AGACAGAGAC TCAGAGTGGC 
CCCAAGCTCA GTTCCAGAGG AGGAAGAAGA AAGATCCAAC AATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006526642.1
CDS	311..715
Translation	MNAPGGRRKEGRRTHRPREQDRNVQLSKALSYALRHGALKLGLPMRAGIRPNCEVAVFIDGPLALTDGIPFFCSANGVILTPGNAEGFLLPKYFKEALQLRPTRKPLSLAGDKETETQSGPKLSSRGGRRKIQQ

Target ORF information:

RefSeq Version	XM_006526579.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus tRNA phosphotransferase 1 (Trpt1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006526579.3

ORF Insert Sequence:

ATGAACGCCC CTGGAGGAAG GAGGAAGGAA GGTAGAAGGA CTCATAGACC CCGGGAACAG 
GACCGAAATG TGCAGCTGTC TAAGGCTCTG TCCTATGCCC TTCGCCACGG GGCCTTGAAG 
CTGGGACTTC CCATGCGAGC TGGCATTCGT CCAAATTGTG AGGTGGCGGT GTTCATCGAT 
GGACCCCTAG CCCTCACAGA TGGAATCCCT TTCTTCTGCT CCGCCAATGG TGTGATTCTG 
ACTCCAGGGA ATGCTGAAGG CTTCCTGCTT CCCAAGTACT TCAAGGAGGC CCTGCAGCTT 
CGACCTACAC GAAAGCCTCT CTCCTTGGCT GGTGATAAAG AGACAGAGAC TCAGAGTGGC 
CCCAAGCTCA GTTCCAGAGG AGGAAGAAGA AAGATCCAAC AATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

An intact unfolded protein response in Trpt1 knockout mice reveals phylogenic divergence in pathways for RNA ligation.
RNA (New York, N.Y.)14(2)225-32(2008 Feb)
Harding HP,Lackey JG,Hsu HC,Zhang Y,Deng J,Xu RM,Damha MJ,Ron D

SAGE2Splice: unmapped SAGE tags reveal novel splice junctions.
PLoS computational biology2(4)e34(2006 Apr)
Kuo BY,Chen Y,Bohacec S,Johansson O,Wasserman WW,Simpson EM

Spermatogonia-dependent expression of testicular genes in mice.
Developmental biology246(2)466-79(2002 Jun)
Tanaka K,Tamura H,Tanaka H,Katoh M,Futamata Y,Seki N,Nishimune Y,Hara T

A functional homolog of a yeast tRNA splicing enzyme is conserved in higher eukaryotes and in Escherichia coli.
Proceedings of the National Academy of Sciences of the United States of America95(24)14136-41(1998 Nov)
Spinelli SL,Malik HS,Consaul SA,Phizicky EM

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Trpt1 cDNA ORF clone, Mus musculus(house mouse)

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gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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