Btaf1 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Btaf1
Entrez Gene ID	107182
Full Name	B-TFIID TATA-box binding protein associated factor 1
Synonyms	AI414500,AI447930,E430027O22Rik,TAF170
General protein information	Preferred Names B-TFIID TATA-box binding protein associated factor 1 Names TATA-binding protein-associated factor 172 BTAF1 RNA polymerase II, B-TFIID transcription factor-associated, (Mot1 homolog, S. cerevisiae) TBP-associated factor 170
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 19 Map Location: 19\|19 C2

mRNA and Protein(s)

mRNA	Protein	Name
XM_006526561.3	XP_006526624.1	TATA-binding protein-associated factor 172 isoform X1
XM_030250684.1	XP_030106544.1	TATA-binding protein-associated factor 172 isoform X2
NM_001080706.1	NP_001074175.1	TATA-binding protein-associated factor 172
XM_017318039.1	XP_017173528.1	TATA-binding protein-associated factor 172 isoform X2
XM_006526561.4	XP_006526624.1	TATA-binding protein-associated factor 172 isoform X1
XM_017318039.2	XP_017173528.1	TATA-binding protein-associated factor 172 isoform X2

Pathways from BioSystems

Homologies

Homo sapiens (human)	BTAF1	NP_003963.1
Danio rerio (zebrafish)	btaf1	XP_005169589.1
Saccharomyces cerevisiae (baker's yeast)	MOT1	NP_015243.1
Gallus gallus (chicken)	BTAF1	XP_421689.3
Eremothecium gossypii	AGOS_AEL256C	NP_984605.2
Magnaporthe oryzae (rice blast fungus)	MGG_04989	XP_003712489.1
Pan troglodytes (chimpanzee)	BTAF1	XP_003312799.1
Canis lupus familiaris (dog)	BTAF1	XP_003640032.1
Bos taurus (cattle)	BTAF1	XP_003587915.2
Arabidopsis thaliana (thale cress)	RGD3	NP_190996.3
Rattus norvegicus (Norway rat)	Btaf1	NP_001178846.1
Drosophila melanogaster (fruit fly)	Hel89B	NP_732097.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP001820	XP_321235.5
Schizosaccharomyces pombe (fission yeast)	mot1	NP_596080.2
	BTAF1	XP_002805795.1
Mus musculus (house mouse)	Btaf1	NP_001074175.1
Kluyveromyces lactis	KLLA0E23717g	XP_455023.1
Neurospora crassa	NCU07556	XP_958601.1
Xenopus tropicalis (tropical clawed frog)	btaf1	XP_002939139.2

Gene Ontology
Bibliography

Related articles in PubMed

Mot1, Ino80C, and NC2 Function Coordinately to Regulate Pervasive Transcription in Yeast and Mammals.
Xue Y, Pradhan SK, Sun F, Chronis C, Tran N, Su T, Van C, Vashisht A, Wohlschlegel J, Peterson CL, Timmers HTM, Kurdistani SK, Carey MF
Molecular cell67(4)594-607.e4(2017 Aug)

An ENU-induced point mutation in the mouse Btaf1 gene causes post-gastrulation embryonic lethality and protein instability.
Wansleeben C, van Gurp L, de Graaf P, Mousson F, Marc Timmers HT, Meijlink F
Mechanisms of development128(5-6)279-88(2011 May-Jun)

BayGenomics: a resource of insertional mutations in mouse embryonic stem cells.
Stryke D, Kawamoto M, Huang CC, Johns SJ, King LA, Harper CA, Meng EC, Lee RE, Yee A, L'Italien L, Chuang PT, Young SG, Skarnes WC, Babbitt PC, Ferrin TE
Nucleic acids research31(1)278-81(2003 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Mot1, Ino80 chromatin remodeling complex (Ino80C), and NC2 co-localize on chromatin and coordinately suppress pervasive transcription in murine embryonic stem cells.
Title: Mot1, Ino80C, and NC2 Function Coordinately to Regulate Pervasive Transcription in Yeast and Mammals.

An ENU-induced point mutation in the mouse Btaf1 gene causes post-gastrulation embryonic lethality and protein instability
Title: An ENU-induced point mutation in the mouse Btaf1 gene causes post-gastrulation embryonic lethality and protein instability.

The following Btaf1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Btaf1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu79877	XM_017318039.2 Latest version!	Mus musculus B-TFIID TATA-box binding protein associated factor 1 (Btaf1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OMu79877	XM_030250684.1 Latest version!	Mus musculus B-TFIID TATA-box binding protein associated factor 1 (Btaf1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OMu55167	XM_006526561.4 Latest version!	Mus musculus B-TFIID TATA-box binding protein associated factor 1 (Btaf1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OMu01417	NM_001080706.1 Latest version!	Mus musculus B-TFIID TATA-box binding protein associated factor 1 (Btaf1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
View Old Accession Versions >>
OMu55167	XM_006526561.3	Mus musculus B-TFIID TATA-box binding protein associated factor 1 (Btaf1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$811.30 $1159.00
OMu79877	XM_017318039.1	Mus musculus B-TFIID TATA-box binding protein associated factor 1 (Btaf1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	15-17	$559.30 $799.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Btaf1 ( XM_017318039.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017173528.1 Mus musculus B-TFIID TATA-box binding protein associated factor 1 (Btaf1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OMu79877
Clone ID Related Accession (Same CDS sequence)	XM_030250684.1 , XM_017318039.1 , XM_017318039.2
Accession Version	XM_017318039.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3231bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	TATA-binding protein-associated factor 172 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000085.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGAATG AATGTAAACA GTTTATATCA TCATTAGCTG ATGCACATAT TGAAGTTGGT 
AATAGAGTAA ACAACAATGT TTTAACAATA GATCAAGCAA ACGACCTGGT AACCACTATT 
TTTAATGAAG TCACATCAAC TTTTGATTTA AATCCTCAAG TGTTACAGCA GTTAGATAGT 
AAACGGCATC AGGTCCAAAT GACAGTTGCA GAGACGAACC AGGAGTGGCA AGTACTGCAG 
CTGAGAGTAC ACACGTTTGC TGCGTGTGCT GTTGTGAGCT TGCAGCAGCT TCCGGAGAAA 
CTAAATCCTA TCATAAAACC ATTAATGGAA ACGATTAAAA AAGAAGAGAA TACGCTAGTG 
CAGAACTATG CTGCTCAGTA CATAGCTAAG CTTCTTCAGC AGTGTACAAC AAGGACACCA 
TGCCCTAATT CAAAAGTTAT TAAGAACCTC TGTAGCTCTC TTTGTGTGGA CCCATACCTC 
ACTCCCTGTG TTACGTGTCC AGTACCAACA CAAAGTGGCC AGGAAAATTC TAAAGGATCC 
AACTCAGAAA AGGATGGAAT GCACCATACT GTCACCAAGC ACAGGGGGAT CATTACACTC 
TACAGGCATC AGAAAGCAGC ATTTGCTATC ACCAGCAGGC GAGGTCCCAT CCCCAAAGCA 
ATCAAAGCTC AGATAGCAGA TCTACCTGCA GGAAGTAGTG GGACTATCCT GGTTGAACTT 
GATGAGGGTC AGAAGCCTTA CCTGGTACAG CGGAGAGGAG CTGAATTTGC CTTAACAACT 
ATAGTGAAGC ATTTTGGTGC TGAAATGGCA GTGAAGTTGC CACATCTTTG GGATGCTATG 
GTTGGCCCAT TGAAGTCTAT GATTGACCTA AATAATTTTG ATGGAAAGTC CCTCCTGGAA 
AGAGGTGATG TTCCTGCTCA AGAATTGGTG AATTCCCTGC AAGTGTTTGA AATAGCAGCA 
GCTTCCATGG ACTCTGCACT TCATCCCTTG CTGGTACAGC ACTTGCCTCA TCTTTATATG 
TGCCTTCAGT ACCCCAGCAC TGCAGTGAGG CACATGGCGG CTCGTTGCAT AGGTGTCATG 
AGTAAAATAG CTACCATGGA AACAATGAAT ATTTTTTTGG AGAAGGTACT TCCTTGGTTG 
GGAGCAATTG ATGACAATGT CAAGCAAGAA GGTGCAATTG AAGCACTTGC CTGTGTTATG 
GAACAATTGG ATGTCGGTAT TGTTCCATAT ATTGTCCTTT TAGTTGTTCC TGTATTGGGA 
AGGATGAGTG ATCAGACAGA CAGTGTGAGA TTCATGGCTA CTCAGTGCTT TGCAACACTG 
ATTAGACTGA TGCCACTTGA GGCAGGCATT CCAGACCCTC CAAACATGTC AGAAGAATTA 
ATCCAGTTGA AAGCAAAAGA GCGACACTTT TTAGAGCAAT TGTTAGATGG GAAGAAGTTA 
GAAAATTACA AGATCCCAGT GCCAATCAAT GCTGAACTCA GGAAATATCA GCAGGATGGT 
GTGAACTGGT TAGCCTTTCT CAATAAGTAT AAGCTTCATG GAATTCTGTG TGATGACATG 
GGCTTAGGAA AAACATTACA GTCCATCTGC ATTCTAGCAG GAGATCACTG TCAGAGGGCC 
CAGGAATATG CAAGATCGAA ATTGGCAGAA TGTATGCCAC TTCCTTCCTT GGTGGTTTGT 
CCACCAACAT TAACAGGTCA TTGGGTAGAT GAAGTAGGTA AATTTTGTTC CAGAGAATAT 
CTCAATCCGT TGCATTATAC TGGCCCTCCC ACTGAAAGGA TAAGGTTGCA ACATCAAGTA 
AAAAGGCACA ATCTGATAGT AGCCTCATAT GATGTTGTGA GGAATGACAT AGACTTTTTT 
AGAAATATTA AATTTAACTA CTGTATTCTT GATGAAGGCC ATGTCATAAA AAATGGAAAA 
ACCAAGTTGT CCAAAGCAGT AAAGCAGCTG ACTGCTAATT ACAGGATTAT TCTTTCTGGA 
ACACCAATCC AGAACAATGT TTTGGAGTTG TGGTCATTAT TTGATTTCCT TATGCCTGGA 
TTTTTGGGTA CTGAACGCCA GTTTGCTGCT CGATATGGCA AACCTATATT AGCGAGTAGG 
GATGCACGAA GCTCCAGTCG AGAACAAGAA GCAGGTGTCC TGGCAATGGA CGCTCTGCAC 
CGCCAAGTCC TGCCGTTCCT TCTCAGAAGA ATGAAAGAAG ATGTTTTGCA GGATCTTCCA 
CCTAAAATTA TTCAAGACTA TTACTGTACT CTCAGCCCTC TCCAGGTTCA GCTTTATGAA 
GATTTTGCTA AGTCGCGTGC CAAGTGTGAT GTTGATGAGA CCGTGTCTTC AGCTGCACTT 
TCAGAAGAAA CTGAAAAACC AAAACTTAAA GCGACAGGTC ATGTATTCCA GGCGTTACAA 
TATTTACGTA AGCTCTGCAA CCATCCAGCA CTGGTCTTAA CCCCTCAGCA TCCAGAATTC 
AAGAGCACTA CTGAAAAACT GACCGTTCAG AATTCTTCTC TTCATGATAT TCAACACGCG 
CCTAAGCTCT CAGCCTTGAA ACAACTGCTG TTGGACTGTG GTTTGGGGAA TGGCAGTTCT 
ACAGAGAGTG GCACAGAGTC TGTTGTGGCA CAGCACAGAA TACTGATCTT CTGTCAGCTT 
AAAAGCATGC TTGATATAGT AGAACATGAT CTGCTCAAGC CTCACCTGCC CTCTGTCACT 
TACTTGAGAC TAGATGGCAG CATACCTCCT GGACAGCGGC ATTCCATTGT TTCCCGGTTT 
AACAACGACC CCTCCATCGA TGTTCTTCTA CTCACAACTC ACGTTGGTGG CCTGGGCCTG 
AACTTGACTG GAGCTGACAC TGTGGTGTTT GTGGAGCATG ACTGGAACCC TATGCGAGAT 
CTGCAGGCCA TGGACCGGGC CCATCGTATT GGGCAGAAAC GTGTGGTTAA TGTCTACCGG 
TTGATAACCA GAGGAACACT AGAAGAAAAG ATAATGGGCT TGCAGAAATT CAAGATGAAC 
ATCGCAAATA CTGTTATTAG CCAAGAAAAC TCTAGCTTAC AGAGTATGGG CACTGATCAA 
CTTCTTGACC TGTTCACTCT AGATAAGGAT GGAAAAGCAG AAAAGGCTGA CAGCTCCACT 
TCTGGGAAAG CAAGCATGAA ATCAGTTCTT GAGAACCTGA GTGACCTCTG GGACGCCGAG 
CAGTATGATT CGGAGTACAA CCTGGAGACC TTCATGCGCT CTCTCGAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017173528.1
CDS	129..3359
Translation	MQNECKQFISSLADAHIEVGNRVNNNVLTIDQANDLVTTIFNEVTSTFDLNPQVLQQLDSKRHQVQMTVAETNQEWQVLQLRVHTFAACAVVSLQQLPEKLNPIIKPLMETIKKEENTLVQNYAAQYIAKLLQQCTTRTPCPNSKVIKNLCSSLCVDPYLTPCVTCPVPTQSGQENSKGSNSEKDGMHHTVTKHRGIITLYRHQKAAFAITSRRGPIPKAIKAQIADLPAGSSGTILVELDEGQKPYLVQRRGAEFALTTIVKHFGAEMAVKLPHLWDAMVGPLKSMIDLNNFDGKSLLERGDVPAQELVNSLQVFEIAAASMDSALHPLLVQHLPHLYMCLQYPSTAVRHMAARCIGVMSKIATMETMNIFLEKVLPWLGAIDDNVKQEGAIEALACVMEQLDVGIVPYIVLLVVPVLGRMSDQTDSVRFMATQCFATLIRLMPLEAGIPDPPNMSEELIQLKAKERHFLEQLLDGKKLENYKIPVPINAELRKYQQDGVNWLAFLNKYKLHGILCDDMGLGKTLQSICILAGDHCQRAQEYARSKLAECMPLPSLVVCPPTLTGHWVDEVGKFCSREYLNPLHYTGPPTERIRLQHQVKRHNLIVASYDVVRNDIDFFRNIKFNYCILDEGHVIKNGKTKLSKAVKQLTANYRIILSGTPIQNNVLELWSLFDFLMPGFLGTERQFAARYGKPILASRDARSSSREQEAGVLAMDALHRQVLPFLLRRMKEDVLQDLPPKIIQDYYCTLSPLQVQLYEDFAKSRAKCDVDETVSSAALSEETEKPKLKATGHVFQALQYLRKLCNHPALVLTPQHPEFKSTTEKLTVQNSSLHDIQHAPKLSALKQLLLDCGLGNGSSTESGTESVVAQHRILIFCQLKSMLDIVEHDLLKPHLPSVTYLRLDGSIPPGQRHSIVSRFNNDPSIDVLLLTTHVGGLGLNLTGADTVVFVEHDWNPMRDLQAMDRAHRIGQKRVVNVYRLITRGTLEEKIMGLQKFKMNIANTVISQENSSLQSMGTDQLLDLFTLDKDGKAEKADSSTSGKASMKSVLENLSDLWDAEQYDSEYNLETFMRSLE

Target ORF information:

RefSeq Version	XM_017318039.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus B-TFIID TATA-box binding protein associated factor 1 (Btaf1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017318039.1

ORF Insert Sequence:

ATGCAGAATG AATGTAAACA GTTTATATCA TCATTAGCTG ATGCACATAT TGAAGTTGGT 
AATAGAGTAA ACAACAATGT TTTAACAATA GATCAAGCAA ACGACCTGGT AACCACTATT 
TTTAATGAAG TCACATCAAC TTTTGATTTA AATCCTCAAG TGTTACAGCA GTTAGATAGT 
AAACGGCATC AGGTCCAAAT GACAGTTGCA GAGACGAACC AGGAGTGGCA AGTACTGCAG 
CTGAGAGTAC ACACGTTTGC TGCGTGTGCT GTTGTGAGCT TGCAGCAGCT TCCGGAGAAA 
CTAAATCCTA TCATAAAACC ATTAATGGAA ACGATTAAAA AAGAAGAGAA TACGCTAGTG 
CAGAACTATG CTGCTCAGTA CATAGCTAAG CTTCTTCAGC AGTGTACAAC AAGGACACCA 
TGCCCTAATT CAAAAGTTAT TAAGAACCTC TGTAGCTCTC TTTGTGTGGA CCCATACCTC 
ACTCCCTGTG TTACGTGTCC AGTACCAACA CAAAGTGGCC AGGAAAATTC TAAAGGATCC 
AACTCAGAAA AGGATGGAAT GCACCATACT GTCACCAAGC ACAGGGGGAT CATTACACTC 
TACAGGCATC AGAAAGCAGC ATTTGCTATC ACCAGCAGGC GAGGTCCCAT CCCCAAAGCA 
ATCAAAGCTC AGATAGCAGA TCTACCTGCA GGAAGTAGTG GGACTATCCT GGTTGAACTT 
GATGAGGGTC AGAAGCCTTA CCTGGTACAG CGGAGAGGAG CTGAATTTGC CTTAACAACT 
ATAGTGAAGC ATTTTGGTGC TGAAATGGCA GTGAAGTTGC CACATCTTTG GGATGCTATG 
GTTGGCCCAT TGAAGTCTAT GATTGACCTA AATAATTTTG ATGGAAAGTC CCTCCTGGAA 
AGAGGTGATG TTCCTGCTCA AGAATTGGTG AATTCCCTGC AAGTGTTTGA AATAGCAGCA 
GCTTCCATGG ACTCTGCACT TCATCCCTTG CTGGTACAGC ACTTGCCTCA TCTTTATATG 
TGCCTTCAGT ACCCCAGCAC TGCAGTGAGG CACATGGCGG CTCGTTGCAT AGGTGTCATG 
AGTAAAATAG CTACCATGGA AACAATGAAT ATTTTTTTGG AGAAGGTACT TCCTTGGTTG 
GGAGCAATTG ATGACAATGT CAAGCAAGAA GGTGCAATTG AAGCACTTGC CTGTGTTATG 
GAACAATTGG ATGTCGGTAT TGTTCCATAT ATTGTCCTTT TAGTTGTTCC TGTATTGGGA 
AGGATGAGTG ATCAGACAGA CAGTGTGAGA TTCATGGCTA CTCAGTGCTT TGCAACACTG 
ATTAGACTGA TGCCACTTGA GGCAGGCATT CCAGACCCTC CAAACATGTC AGAAGAATTA 
ATCCAGTTGA AAGCAAAAGA GCGACACTTT TTAGAGCAAT TGTTAGATGG GAAGAAGTTA 
GAAAATTACA AGATCCCAGT GCCAATCAAT GCTGAACTCA GGAAATATCA GCAGGATGGT 
GTGAACTGGT TAGCCTTTCT CAATAAGTAT AAGCTTCATG GAATTCTGTG TGATGACATG 
GGCTTAGGAA AAACATTACA GTCCATCTGC ATTCTAGCAG GAGATCACTG TCAGAGGGCC 
CAGGAATATG CAAGATCGAA ATTGGCAGAA TGTATGCCAC TTCCTTCCTT GGTGGTTTGT 
CCACCAACAT TAACAGGTCA TTGGGTAGAT GAAGTAGGTA AATTTTGTTC CAGAGAATAT 
CTCAATCCGT TGCATTATAC TGGCCCTCCC ACTGAAAGGA TAAGGTTGCA ACATCAAGTA 
AAAAGGCACA ATCTGATAGT AGCCTCATAT GATGTTGTGA GGAATGACAT AGACTTTTTT 
AGAAATATTA AATTTAACTA CTGTATTCTT GATGAAGGCC ATGTCATAAA AAATGGAAAA 
ACCAAGTTGT CCAAAGCAGT AAAGCAGCTG ACTGCTAATT ACAGGATTAT TCTTTCTGGA 
ACACCAATCC AGAACAATGT TTTGGAGTTG TGGTCATTAT TTGATTTCCT TATGCCTGGA 
TTTTTGGGTA CTGAACGCCA GTTTGCTGCT CGATATGGCA AACCTATATT AGCGAGTAGG 
GATGCACGAA GCTCCAGTCG AGAACAAGAA GCAGGTGTCC TGGCAATGGA CGCTCTGCAC 
CGCCAAGTCC TGCCGTTCCT TCTCAGAAGA ATGAAAGAAG ATGTTTTGCA GGATCTTCCA 
CCTAAAATTA TTCAAGACTA TTACTGTACT CTCAGCCCTC TCCAGGTTCA GCTTTATGAA 
GATTTTGCTA AGTCGCGTGC CAAGTGTGAT GTTGATGAGA CCGTGTCTTC AGCTGCACTT 
TCAGAAGAAA CTGAAAAACC AAAACTTAAA GCGACAGGTC ATGTATTCCA GGCGTTACAA 
TATTTACGTA AGCTCTGCAA CCATCCAGCA CTGGTCTTAA CCCCTCAGCA TCCAGAATTC 
AAGAGCACTA CTGAAAAACT GACCGTTCAG AATTCTTCTC TTCATGATAT TCAACACGCG 
CCTAAGCTCT CAGCCTTGAA ACAACTGCTG TTGGACTGTG GTTTGGGGAA TGGCAGTTCT 
ACAGAGAGTG GCACAGAGTC TGTTGTGGCA CAGCACAGAA TACTGATCTT CTGTCAGCTT 
AAAAGCATGC TTGATATAGT AGAACATGAT CTGCTCAAGC CTCACCTGCC CTCTGTCACT 
TACTTGAGAC TAGATGGCAG CATACCTCCT GGACAGCGGC ATTCCATTGT TTCCCGGTTT 
AACAACGACC CCTCCATCGA TGTTCTTCTA CTCACAACTC ACGTTGGTGG CCTGGGCCTG 
AACTTGACTG GAGCTGACAC TGTGGTGTTT GTGGAGCATG ACTGGAACCC TATGCGAGAT 
CTGCAGGCCA TGGACCGGGC CCATCGTATT GGGCAGAAAC GTGTGGTTAA TGTCTACCGG 
TTGATAACCA GAGGAACACT AGAAGAAAAG ATAATGGGCT TGCAGAAATT CAAGATGAAC 
ATCGCAAATA CTGTTATTAG CCAAGAAAAC TCTAGCTTAC AGAGTATGGG CACTGATCAA 
CTTCTTGACC TGTTCACTCT AGATAAGGAT GGAAAAGCAG AAAAGGCTGA CAGCTCCACT 
TCTGGGAAAG CAAGCATGAA ATCAGTTCTT GAGAACCTGA GTGACCTCTG GGACGCCGAG 
CAGTATGATT CGGAGTACAA CCTGGAGACC TTCATGCGCT CTCTCGAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Btaf1 ( XM_030250684.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030106544.1 Mus musculus B-TFIID TATA-box binding protein associated factor 1 (Btaf1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OMu79877
Clone ID Related Accession (Same CDS sequence)	XM_030250684.1 , XM_017318039.1 , XM_017318039.2
Accession Version	XM_030250684.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3231bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	TATA-binding protein-associated factor 172 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000085.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGAATG AATGTAAACA GTTTATATCA TCATTAGCTG ATGCACATAT TGAAGTTGGT 
AATAGAGTAA ACAACAATGT TTTAACAATA GATCAAGCAA ACGACCTGGT AACCACTATT 
TTTAATGAAG TCACATCAAC TTTTGATTTA AATCCTCAAG TGTTACAGCA GTTAGATAGT 
AAACGGCATC AGGTCCAAAT GACAGTTGCA GAGACGAACC AGGAGTGGCA AGTACTGCAG 
CTGAGAGTAC ACACGTTTGC TGCGTGTGCT GTTGTGAGCT TGCAGCAGCT TCCGGAGAAA 
CTAAATCCTA TCATAAAACC ATTAATGGAA ACGATTAAAA AAGAAGAGAA TACGCTAGTG 
CAGAACTATG CTGCTCAGTA CATAGCTAAG CTTCTTCAGC AGTGTACAAC AAGGACACCA 
TGCCCTAATT CAAAAGTTAT TAAGAACCTC TGTAGCTCTC TTTGTGTGGA CCCATACCTC 
ACTCCCTGTG TTACGTGTCC AGTACCAACA CAAAGTGGCC AGGAAAATTC TAAAGGATCC 
AACTCAGAAA AGGATGGAAT GCACCATACT GTCACCAAGC ACAGGGGGAT CATTACACTC 
TACAGGCATC AGAAAGCAGC ATTTGCTATC ACCAGCAGGC GAGGTCCCAT CCCCAAAGCA 
ATCAAAGCTC AGATAGCAGA TCTACCTGCA GGAAGTAGTG GGACTATCCT GGTTGAACTT 
GATGAGGGTC AGAAGCCTTA CCTGGTACAG CGGAGAGGAG CTGAATTTGC CTTAACAACT 
ATAGTGAAGC ATTTTGGTGC TGAAATGGCA GTGAAGTTGC CACATCTTTG GGATGCTATG 
GTTGGCCCAT TGAAGTCTAT GATTGACCTA AATAATTTTG ATGGAAAGTC CCTCCTGGAA 
AGAGGTGATG TTCCTGCTCA AGAATTGGTG AATTCCCTGC AAGTGTTTGA AATAGCAGCA 
GCTTCCATGG ACTCTGCACT TCATCCCTTG CTGGTACAGC ACTTGCCTCA TCTTTATATG 
TGCCTTCAGT ACCCCAGCAC TGCAGTGAGG CACATGGCGG CTCGTTGCAT AGGTGTCATG 
AGTAAAATAG CTACCATGGA AACAATGAAT ATTTTTTTGG AGAAGGTACT TCCTTGGTTG 
GGAGCAATTG ATGACAATGT CAAGCAAGAA GGTGCAATTG AAGCACTTGC CTGTGTTATG 
GAACAATTGG ATGTCGGTAT TGTTCCATAT ATTGTCCTTT TAGTTGTTCC TGTATTGGGA 
AGGATGAGTG ATCAGACAGA CAGTGTGAGA TTCATGGCTA CTCAGTGCTT TGCAACACTG 
ATTAGACTGA TGCCACTTGA GGCAGGCATT CCAGACCCTC CAAACATGTC AGAAGAATTA 
ATCCAGTTGA AAGCAAAAGA GCGACACTTT TTAGAGCAAT TGTTAGATGG GAAGAAGTTA 
GAAAATTACA AGATCCCAGT GCCAATCAAT GCTGAACTCA GGAAATATCA GCAGGATGGT 
GTGAACTGGT TAGCCTTTCT CAATAAGTAT AAGCTTCATG GAATTCTGTG TGATGACATG 
GGCTTAGGAA AAACATTACA GTCCATCTGC ATTCTAGCAG GAGATCACTG TCAGAGGGCC 
CAGGAATATG CAAGATCGAA ATTGGCAGAA TGTATGCCAC TTCCTTCCTT GGTGGTTTGT 
CCACCAACAT TAACAGGTCA TTGGGTAGAT GAAGTAGGTA AATTTTGTTC CAGAGAATAT 
CTCAATCCGT TGCATTATAC TGGCCCTCCC ACTGAAAGGA TAAGGTTGCA ACATCAAGTA 
AAAAGGCACA ATCTGATAGT AGCCTCATAT GATGTTGTGA GGAATGACAT AGACTTTTTT 
AGAAATATTA AATTTAACTA CTGTATTCTT GATGAAGGCC ATGTCATAAA AAATGGAAAA 
ACCAAGTTGT CCAAAGCAGT AAAGCAGCTG ACTGCTAATT ACAGGATTAT TCTTTCTGGA 
ACACCAATCC AGAACAATGT TTTGGAGTTG TGGTCATTAT TTGATTTCCT TATGCCTGGA 
TTTTTGGGTA CTGAACGCCA GTTTGCTGCT CGATATGGCA AACCTATATT AGCGAGTAGG 
GATGCACGAA GCTCCAGTCG AGAACAAGAA GCAGGTGTCC TGGCAATGGA CGCTCTGCAC 
CGCCAAGTCC TGCCGTTCCT TCTCAGAAGA ATGAAAGAAG ATGTTTTGCA GGATCTTCCA 
CCTAAAATTA TTCAAGACTA TTACTGTACT CTCAGCCCTC TCCAGGTTCA GCTTTATGAA 
GATTTTGCTA AGTCGCGTGC CAAGTGTGAT GTTGATGAGA CCGTGTCTTC AGCTGCACTT 
TCAGAAGAAA CTGAAAAACC AAAACTTAAA GCGACAGGTC ATGTATTCCA GGCGTTACAA 
TATTTACGTA AGCTCTGCAA CCATCCAGCA CTGGTCTTAA CCCCTCAGCA TCCAGAATTC 
AAGAGCACTA CTGAAAAACT GACCGTTCAG AATTCTTCTC TTCATGATAT TCAACACGCG 
CCTAAGCTCT CAGCCTTGAA ACAACTGCTG TTGGACTGTG GTTTGGGGAA TGGCAGTTCT 
ACAGAGAGTG GCACAGAGTC TGTTGTGGCA CAGCACAGAA TACTGATCTT CTGTCAGCTT 
AAAAGCATGC TTGATATAGT AGAACATGAT CTGCTCAAGC CTCACCTGCC CTCTGTCACT 
TACTTGAGAC TAGATGGCAG CATACCTCCT GGACAGCGGC ATTCCATTGT TTCCCGGTTT 
AACAACGACC CCTCCATCGA TGTTCTTCTA CTCACAACTC ACGTTGGTGG CCTGGGCCTG 
AACTTGACTG GAGCTGACAC TGTGGTGTTT GTGGAGCATG ACTGGAACCC TATGCGAGAT 
CTGCAGGCCA TGGACCGGGC CCATCGTATT GGGCAGAAAC GTGTGGTTAA TGTCTACCGG 
TTGATAACCA GAGGAACACT AGAAGAAAAG ATAATGGGCT TGCAGAAATT CAAGATGAAC 
ATCGCAAATA CTGTTATTAG CCAAGAAAAC TCTAGCTTAC AGAGTATGGG CACTGATCAA 
CTTCTTGACC TGTTCACTCT AGATAAGGAT GGAAAAGCAG AAAAGGCTGA CAGCTCCACT 
TCTGGGAAAG CAAGCATGAA ATCAGTTCTT GAGAACCTGA GTGACCTCTG GGACGCCGAG 
CAGTATGATT CGGAGTACAA CCTGGAGACC TTCATGCGCT CTCTCGAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030106544.1
CDS	1250..4480
Translation	MQNECKQFISSLADAHIEVGNRVNNNVLTIDQANDLVTTIFNEVTSTFDLNPQVLQQLDSKRHQVQMTVAETNQEWQVLQLRVHTFAACAVVSLQQLPEKLNPIIKPLMETIKKEENTLVQNYAAQYIAKLLQQCTTRTPCPNSKVIKNLCSSLCVDPYLTPCVTCPVPTQSGQENSKGSNSEKDGMHHTVTKHRGIITLYRHQKAAFAITSRRGPIPKAIKAQIADLPAGSSGTILVELDEGQKPYLVQRRGAEFALTTIVKHFGAEMAVKLPHLWDAMVGPLKSMIDLNNFDGKSLLERGDVPAQELVNSLQVFEIAAASMDSALHPLLVQHLPHLYMCLQYPSTAVRHMAARCIGVMSKIATMETMNIFLEKVLPWLGAIDDNVKQEGAIEALACVMEQLDVGIVPYIVLLVVPVLGRMSDQTDSVRFMATQCFATLIRLMPLEAGIPDPPNMSEELIQLKAKERHFLEQLLDGKKLENYKIPVPINAELRKYQQDGVNWLAFLNKYKLHGILCDDMGLGKTLQSICILAGDHCQRAQEYARSKLAECMPLPSLVVCPPTLTGHWVDEVGKFCSREYLNPLHYTGPPTERIRLQHQVKRHNLIVASYDVVRNDIDFFRNIKFNYCILDEGHVIKNGKTKLSKAVKQLTANYRIILSGTPIQNNVLELWSLFDFLMPGFLGTERQFAARYGKPILASRDARSSSREQEAGVLAMDALHRQVLPFLLRRMKEDVLQDLPPKIIQDYYCTLSPLQVQLYEDFAKSRAKCDVDETVSSAALSEETEKPKLKATGHVFQALQYLRKLCNHPALVLTPQHPEFKSTTEKLTVQNSSLHDIQHAPKLSALKQLLLDCGLGNGSSTESGTESVVAQHRILIFCQLKSMLDIVEHDLLKPHLPSVTYLRLDGSIPPGQRHSIVSRFNNDPSIDVLLLTTHVGGLGLNLTGADTVVFVEHDWNPMRDLQAMDRAHRIGQKRVVNVYRLITRGTLEEKIMGLQKFKMNIANTVISQENSSLQSMGTDQLLDLFTLDKDGKAEKADSSTSGKASMKSVLENLSDLWDAEQYDSEYNLETFMRSLE

Target ORF information:

RefSeq Version	XM_030250684.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus B-TFIID TATA-box binding protein associated factor 1 (Btaf1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030250684.1

ORF Insert Sequence:

ATGCAGAATG AATGTAAACA GTTTATATCA TCATTAGCTG ATGCACATAT TGAAGTTGGT 
AATAGAGTAA ACAACAATGT TTTAACAATA GATCAAGCAA ACGACCTGGT AACCACTATT 
TTTAATGAAG TCACATCAAC TTTTGATTTA AATCCTCAAG TGTTACAGCA GTTAGATAGT 
AAACGGCATC AGGTCCAAAT GACAGTTGCA GAGACGAACC AGGAGTGGCA AGTACTGCAG 
CTGAGAGTAC ACACGTTTGC TGCGTGTGCT GTTGTGAGCT TGCAGCAGCT TCCGGAGAAA 
CTAAATCCTA TCATAAAACC ATTAATGGAA ACGATTAAAA AAGAAGAGAA TACGCTAGTG 
CAGAACTATG CTGCTCAGTA CATAGCTAAG CTTCTTCAGC AGTGTACAAC AAGGACACCA 
TGCCCTAATT CAAAAGTTAT TAAGAACCTC TGTAGCTCTC TTTGTGTGGA CCCATACCTC 
ACTCCCTGTG TTACGTGTCC AGTACCAACA CAAAGTGGCC AGGAAAATTC TAAAGGATCC 
AACTCAGAAA AGGATGGAAT GCACCATACT GTCACCAAGC ACAGGGGGAT CATTACACTC 
TACAGGCATC AGAAAGCAGC ATTTGCTATC ACCAGCAGGC GAGGTCCCAT CCCCAAAGCA 
ATCAAAGCTC AGATAGCAGA TCTACCTGCA GGAAGTAGTG GGACTATCCT GGTTGAACTT 
GATGAGGGTC AGAAGCCTTA CCTGGTACAG CGGAGAGGAG CTGAATTTGC CTTAACAACT 
ATAGTGAAGC ATTTTGGTGC TGAAATGGCA GTGAAGTTGC CACATCTTTG GGATGCTATG 
GTTGGCCCAT TGAAGTCTAT GATTGACCTA AATAATTTTG ATGGAAAGTC CCTCCTGGAA 
AGAGGTGATG TTCCTGCTCA AGAATTGGTG AATTCCCTGC AAGTGTTTGA AATAGCAGCA 
GCTTCCATGG ACTCTGCACT TCATCCCTTG CTGGTACAGC ACTTGCCTCA TCTTTATATG 
TGCCTTCAGT ACCCCAGCAC TGCAGTGAGG CACATGGCGG CTCGTTGCAT AGGTGTCATG 
AGTAAAATAG CTACCATGGA AACAATGAAT ATTTTTTTGG AGAAGGTACT TCCTTGGTTG 
GGAGCAATTG ATGACAATGT CAAGCAAGAA GGTGCAATTG AAGCACTTGC CTGTGTTATG 
GAACAATTGG ATGTCGGTAT TGTTCCATAT ATTGTCCTTT TAGTTGTTCC TGTATTGGGA 
AGGATGAGTG ATCAGACAGA CAGTGTGAGA TTCATGGCTA CTCAGTGCTT TGCAACACTG 
ATTAGACTGA TGCCACTTGA GGCAGGCATT CCAGACCCTC CAAACATGTC AGAAGAATTA 
ATCCAGTTGA AAGCAAAAGA GCGACACTTT TTAGAGCAAT TGTTAGATGG GAAGAAGTTA 
GAAAATTACA AGATCCCAGT GCCAATCAAT GCTGAACTCA GGAAATATCA GCAGGATGGT 
GTGAACTGGT TAGCCTTTCT CAATAAGTAT AAGCTTCATG GAATTCTGTG TGATGACATG 
GGCTTAGGAA AAACATTACA GTCCATCTGC ATTCTAGCAG GAGATCACTG TCAGAGGGCC 
CAGGAATATG CAAGATCGAA ATTGGCAGAA TGTATGCCAC TTCCTTCCTT GGTGGTTTGT 
CCACCAACAT TAACAGGTCA TTGGGTAGAT GAAGTAGGTA AATTTTGTTC CAGAGAATAT 
CTCAATCCGT TGCATTATAC TGGCCCTCCC ACTGAAAGGA TAAGGTTGCA ACATCAAGTA 
AAAAGGCACA ATCTGATAGT AGCCTCATAT GATGTTGTGA GGAATGACAT AGACTTTTTT 
AGAAATATTA AATTTAACTA CTGTATTCTT GATGAAGGCC ATGTCATAAA AAATGGAAAA 
ACCAAGTTGT CCAAAGCAGT AAAGCAGCTG ACTGCTAATT ACAGGATTAT TCTTTCTGGA 
ACACCAATCC AGAACAATGT TTTGGAGTTG TGGTCATTAT TTGATTTCCT TATGCCTGGA 
TTTTTGGGTA CTGAACGCCA GTTTGCTGCT CGATATGGCA AACCTATATT AGCGAGTAGG 
GATGCACGAA GCTCCAGTCG AGAACAAGAA GCAGGTGTCC TGGCAATGGA CGCTCTGCAC 
CGCCAAGTCC TGCCGTTCCT TCTCAGAAGA ATGAAAGAAG ATGTTTTGCA GGATCTTCCA 
CCTAAAATTA TTCAAGACTA TTACTGTACT CTCAGCCCTC TCCAGGTTCA GCTTTATGAA 
GATTTTGCTA AGTCGCGTGC CAAGTGTGAT GTTGATGAGA CCGTGTCTTC AGCTGCACTT 
TCAGAAGAAA CTGAAAAACC AAAACTTAAA GCGACAGGTC ATGTATTCCA GGCGTTACAA 
TATTTACGTA AGCTCTGCAA CCATCCAGCA CTGGTCTTAA CCCCTCAGCA TCCAGAATTC 
AAGAGCACTA CTGAAAAACT GACCGTTCAG AATTCTTCTC TTCATGATAT TCAACACGCG 
CCTAAGCTCT CAGCCTTGAA ACAACTGCTG TTGGACTGTG GTTTGGGGAA TGGCAGTTCT 
ACAGAGAGTG GCACAGAGTC TGTTGTGGCA CAGCACAGAA TACTGATCTT CTGTCAGCTT 
AAAAGCATGC TTGATATAGT AGAACATGAT CTGCTCAAGC CTCACCTGCC CTCTGTCACT 
TACTTGAGAC TAGATGGCAG CATACCTCCT GGACAGCGGC ATTCCATTGT TTCCCGGTTT 
AACAACGACC CCTCCATCGA TGTTCTTCTA CTCACAACTC ACGTTGGTGG CCTGGGCCTG 
AACTTGACTG GAGCTGACAC TGTGGTGTTT GTGGAGCATG ACTGGAACCC TATGCGAGAT 
CTGCAGGCCA TGGACCGGGC CCATCGTATT GGGCAGAAAC GTGTGGTTAA TGTCTACCGG 
TTGATAACCA GAGGAACACT AGAAGAAAAG ATAATGGGCT TGCAGAAATT CAAGATGAAC 
ATCGCAAATA CTGTTATTAG CCAAGAAAAC TCTAGCTTAC AGAGTATGGG CACTGATCAA 
CTTCTTGACC TGTTCACTCT AGATAAGGAT GGAAAAGCAG AAAAGGCTGA CAGCTCCACT 
TCTGGGAAAG CAAGCATGAA ATCAGTTCTT GAGAACCTGA GTGACCTCTG GGACGCCGAG 
CAGTATGATT CGGAGTACAA CCTGGAGACC TTCATGCGCT CTCTCGAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Btaf1 ( XM_017318039.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017173528.1 Mus musculus B-TFIID TATA-box binding protein associated factor 1 (Btaf1), transcript variant X3, mRNA.

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CloneID	OMu79877
Clone ID Related Accession (Same CDS sequence)	XM_030250684.1 , XM_017318039.1 , XM_017318039.2
Accession Version	XM_017318039.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3231bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	TATA-binding protein-associated factor 172 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000085.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_017318039.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGAATG AATGTAAACA GTTTATATCA TCATTAGCTG ATGCACATAT TGAAGTTGGT 
AATAGAGTAA ACAACAATGT TTTAACAATA GATCAAGCAA ACGACCTGGT AACCACTATT 
TTTAATGAAG TCACATCAAC TTTTGATTTA AATCCTCAAG TGTTACAGCA GTTAGATAGT 
AAACGGCATC AGGTCCAAAT GACAGTTGCA GAGACGAACC AGGAGTGGCA AGTACTGCAG 
CTGAGAGTAC ACACGTTTGC TGCGTGTGCT GTTGTGAGCT TGCAGCAGCT TCCGGAGAAA 
CTAAATCCTA TCATAAAACC ATTAATGGAA ACGATTAAAA AAGAAGAGAA TACGCTAGTG 
CAGAACTATG CTGCTCAGTA CATAGCTAAG CTTCTTCAGC AGTGTACAAC AAGGACACCA 
TGCCCTAATT CAAAAGTTAT TAAGAACCTC TGTAGCTCTC TTTGTGTGGA CCCATACCTC 
ACTCCCTGTG TTACGTGTCC AGTACCAACA CAAAGTGGCC AGGAAAATTC TAAAGGATCC 
AACTCAGAAA AGGATGGAAT GCACCATACT GTCACCAAGC ACAGGGGGAT CATTACACTC 
TACAGGCATC AGAAAGCAGC ATTTGCTATC ACCAGCAGGC GAGGTCCCAT CCCCAAAGCA 
ATCAAAGCTC AGATAGCAGA TCTACCTGCA GGAAGTAGTG GGACTATCCT GGTTGAACTT 
GATGAGGGTC AGAAGCCTTA CCTGGTACAG CGGAGAGGAG CTGAATTTGC CTTAACAACT 
ATAGTGAAGC ATTTTGGTGC TGAAATGGCA GTGAAGTTGC CACATCTTTG GGATGCTATG 
GTTGGCCCAT TGAAGTCTAT GATTGACCTA AATAATTTTG ATGGAAAGTC CCTCCTGGAA 
AGAGGTGATG TTCCTGCTCA AGAATTGGTG AATTCCCTGC AAGTGTTTGA AATAGCAGCA 
GCTTCCATGG ACTCTGCACT TCATCCCTTG CTGGTACAGC ACTTGCCTCA TCTTTATATG 
TGCCTTCAGT ACCCCAGCAC TGCAGTGAGG CACATGGCGG CTCGTTGCAT AGGTGTCATG 
AGTAAAATAG CTACCATGGA AACAATGAAT ATTTTTTTGG AGAAGGTACT TCCTTGGTTG 
GGAGCAATTG ATGACAATGT CAAGCAAGAA GGTGCAATTG AAGCACTTGC CTGTGTTATG 
GAACAATTGG ATGTCGGTAT TGTTCCATAT ATTGTCCTTT TAGTTGTTCC TGTATTGGGA 
AGGATGAGTG ATCAGACAGA CAGTGTGAGA TTCATGGCTA CTCAGTGCTT TGCAACACTG 
ATTAGACTGA TGCCACTTGA GGCAGGCATT CCAGACCCTC CAAACATGTC AGAAGAATTA 
ATCCAGTTGA AAGCAAAAGA GCGACACTTT TTAGAGCAAT TGTTAGATGG GAAGAAGTTA 
GAAAATTACA AGATCCCAGT GCCAATCAAT GCTGAACTCA GGAAATATCA GCAGGATGGT 
GTGAACTGGT TAGCCTTTCT CAATAAGTAT AAGCTTCATG GAATTCTGTG TGATGACATG 
GGCTTAGGAA AAACATTACA GTCCATCTGC ATTCTAGCAG GAGATCACTG TCAGAGGGCC 
CAGGAATATG CAAGATCGAA ATTGGCAGAA TGTATGCCAC TTCCTTCCTT GGTGGTTTGT 
CCACCAACAT TAACAGGTCA TTGGGTAGAT GAAGTAGGTA AATTTTGTTC CAGAGAATAT 
CTCAATCCGT TGCATTATAC TGGCCCTCCC ACTGAAAGGA TAAGGTTGCA ACATCAAGTA 
AAAAGGCACA ATCTGATAGT AGCCTCATAT GATGTTGTGA GGAATGACAT AGACTTTTTT 
AGAAATATTA AATTTAACTA CTGTATTCTT GATGAAGGCC ATGTCATAAA AAATGGAAAA 
ACCAAGTTGT CCAAAGCAGT AAAGCAGCTG ACTGCTAATT ACAGGATTAT TCTTTCTGGA 
ACACCAATCC AGAACAATGT TTTGGAGTTG TGGTCATTAT TTGATTTCCT TATGCCTGGA 
TTTTTGGGTA CTGAACGCCA GTTTGCTGCT CGATATGGCA AACCTATATT AGCGAGTAGG 
GATGCACGAA GCTCCAGTCG AGAACAAGAA GCAGGTGTCC TGGCAATGGA CGCTCTGCAC 
CGCCAAGTCC TGCCGTTCCT TCTCAGAAGA ATGAAAGAAG ATGTTTTGCA GGATCTTCCA 
CCTAAAATTA TTCAAGACTA TTACTGTACT CTCAGCCCTC TCCAGGTTCA GCTTTATGAA 
GATTTTGCTA AGTCGCGTGC CAAGTGTGAT GTTGATGAGA CCGTGTCTTC AGCTGCACTT 
TCAGAAGAAA CTGAAAAACC AAAACTTAAA GCGACAGGTC ATGTATTCCA GGCGTTACAA 
TATTTACGTA AGCTCTGCAA CCATCCAGCA CTGGTCTTAA CCCCTCAGCA TCCAGAATTC 
AAGAGCACTA CTGAAAAACT GACCGTTCAG AATTCTTCTC TTCATGATAT TCAACACGCG 
CCTAAGCTCT CAGCCTTGAA ACAACTGCTG TTGGACTGTG GTTTGGGGAA TGGCAGTTCT 
ACAGAGAGTG GCACAGAGTC TGTTGTGGCA CAGCACAGAA TACTGATCTT CTGTCAGCTT 
AAAAGCATGC TTGATATAGT AGAACATGAT CTGCTCAAGC CTCACCTGCC CTCTGTCACT 
TACTTGAGAC TAGATGGCAG CATACCTCCT GGACAGCGGC ATTCCATTGT TTCCCGGTTT 
AACAACGACC CCTCCATCGA TGTTCTTCTA CTCACAACTC ACGTTGGTGG CCTGGGCCTG 
AACTTGACTG GAGCTGACAC TGTGGTGTTT GTGGAGCATG ACTGGAACCC TATGCGAGAT 
CTGCAGGCCA TGGACCGGGC CCATCGTATT GGGCAGAAAC GTGTGGTTAA TGTCTACCGG 
TTGATAACCA GAGGAACACT AGAAGAAAAG ATAATGGGCT TGCAGAAATT CAAGATGAAC 
ATCGCAAATA CTGTTATTAG CCAAGAAAAC TCTAGCTTAC AGAGTATGGG CACTGATCAA 
CTTCTTGACC TGTTCACTCT AGATAAGGAT GGAAAAGCAG AAAAGGCTGA CAGCTCCACT 
TCTGGGAAAG CAAGCATGAA ATCAGTTCTT GAGAACCTGA GTGACCTCTG GGACGCCGAG 
CAGTATGATT CGGAGTACAA CCTGGAGACC TTCATGCGCT CTCTCGAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017173528.1
CDS	770..4000
Translation	MQNECKQFISSLADAHIEVGNRVNNNVLTIDQANDLVTTIFNEVTSTFDLNPQVLQQLDSKRHQVQMTVAETNQEWQVLQLRVHTFAACAVVSLQQLPEKLNPIIKPLMETIKKEENTLVQNYAAQYIAKLLQQCTTRTPCPNSKVIKNLCSSLCVDPYLTPCVTCPVPTQSGQENSKGSNSEKDGMHHTVTKHRGIITLYRHQKAAFAITSRRGPIPKAIKAQIADLPAGSSGTILVELDEGQKPYLVQRRGAEFALTTIVKHFGAEMAVKLPHLWDAMVGPLKSMIDLNNFDGKSLLERGDVPAQELVNSLQVFEIAAASMDSALHPLLVQHLPHLYMCLQYPSTAVRHMAARCIGVMSKIATMETMNIFLEKVLPWLGAIDDNVKQEGAIEALACVMEQLDVGIVPYIVLLVVPVLGRMSDQTDSVRFMATQCFATLIRLMPLEAGIPDPPNMSEELIQLKAKERHFLEQLLDGKKLENYKIPVPINAELRKYQQDGVNWLAFLNKYKLHGILCDDMGLGKTLQSICILAGDHCQRAQEYARSKLAECMPLPSLVVCPPTLTGHWVDEVGKFCSREYLNPLHYTGPPTERIRLQHQVKRHNLIVASYDVVRNDIDFFRNIKFNYCILDEGHVIKNGKTKLSKAVKQLTANYRIILSGTPIQNNVLELWSLFDFLMPGFLGTERQFAARYGKPILASRDARSSSREQEAGVLAMDALHRQVLPFLLRRMKEDVLQDLPPKIIQDYYCTLSPLQVQLYEDFAKSRAKCDVDETVSSAALSEETEKPKLKATGHVFQALQYLRKLCNHPALVLTPQHPEFKSTTEKLTVQNSSLHDIQHAPKLSALKQLLLDCGLGNGSSTESGTESVVAQHRILIFCQLKSMLDIVEHDLLKPHLPSVTYLRLDGSIPPGQRHSIVSRFNNDPSIDVLLLTTHVGGLGLNLTGADTVVFVEHDWNPMRDLQAMDRAHRIGQKRVVNVYRLITRGTLEEKIMGLQKFKMNIANTVISQENSSLQSMGTDQLLDLFTLDKDGKAEKADSSTSGKASMKSVLENLSDLWDAEQYDSEYNLETFMRSLE

Target ORF information:

RefSeq Version	XM_017318039.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus B-TFIID TATA-box binding protein associated factor 1 (Btaf1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017318039.2

ORF Insert Sequence:

ATGCAGAATG AATGTAAACA GTTTATATCA TCATTAGCTG ATGCACATAT TGAAGTTGGT 
AATAGAGTAA ACAACAATGT TTTAACAATA GATCAAGCAA ACGACCTGGT AACCACTATT 
TTTAATGAAG TCACATCAAC TTTTGATTTA AATCCTCAAG TGTTACAGCA GTTAGATAGT 
AAACGGCATC AGGTCCAAAT GACAGTTGCA GAGACGAACC AGGAGTGGCA AGTACTGCAG 
CTGAGAGTAC ACACGTTTGC TGCGTGTGCT GTTGTGAGCT TGCAGCAGCT TCCGGAGAAA 
CTAAATCCTA TCATAAAACC ATTAATGGAA ACGATTAAAA AAGAAGAGAA TACGCTAGTG 
CAGAACTATG CTGCTCAGTA CATAGCTAAG CTTCTTCAGC AGTGTACAAC AAGGACACCA 
TGCCCTAATT CAAAAGTTAT TAAGAACCTC TGTAGCTCTC TTTGTGTGGA CCCATACCTC 
ACTCCCTGTG TTACGTGTCC AGTACCAACA CAAAGTGGCC AGGAAAATTC TAAAGGATCC 
AACTCAGAAA AGGATGGAAT GCACCATACT GTCACCAAGC ACAGGGGGAT CATTACACTC 
TACAGGCATC AGAAAGCAGC ATTTGCTATC ACCAGCAGGC GAGGTCCCAT CCCCAAAGCA 
ATCAAAGCTC AGATAGCAGA TCTACCTGCA GGAAGTAGTG GGACTATCCT GGTTGAACTT 
GATGAGGGTC AGAAGCCTTA CCTGGTACAG CGGAGAGGAG CTGAATTTGC CTTAACAACT 
ATAGTGAAGC ATTTTGGTGC TGAAATGGCA GTGAAGTTGC CACATCTTTG GGATGCTATG 
GTTGGCCCAT TGAAGTCTAT GATTGACCTA AATAATTTTG ATGGAAAGTC CCTCCTGGAA 
AGAGGTGATG TTCCTGCTCA AGAATTGGTG AATTCCCTGC AAGTGTTTGA AATAGCAGCA 
GCTTCCATGG ACTCTGCACT TCATCCCTTG CTGGTACAGC ACTTGCCTCA TCTTTATATG 
TGCCTTCAGT ACCCCAGCAC TGCAGTGAGG CACATGGCGG CTCGTTGCAT AGGTGTCATG 
AGTAAAATAG CTACCATGGA AACAATGAAT ATTTTTTTGG AGAAGGTACT TCCTTGGTTG 
GGAGCAATTG ATGACAATGT CAAGCAAGAA GGTGCAATTG AAGCACTTGC CTGTGTTATG 
GAACAATTGG ATGTCGGTAT TGTTCCATAT ATTGTCCTTT TAGTTGTTCC TGTATTGGGA 
AGGATGAGTG ATCAGACAGA CAGTGTGAGA TTCATGGCTA CTCAGTGCTT TGCAACACTG 
ATTAGACTGA TGCCACTTGA GGCAGGCATT CCAGACCCTC CAAACATGTC AGAAGAATTA 
ATCCAGTTGA AAGCAAAAGA GCGACACTTT TTAGAGCAAT TGTTAGATGG GAAGAAGTTA 
GAAAATTACA AGATCCCAGT GCCAATCAAT GCTGAACTCA GGAAATATCA GCAGGATGGT 
GTGAACTGGT TAGCCTTTCT CAATAAGTAT AAGCTTCATG GAATTCTGTG TGATGACATG 
GGCTTAGGAA AAACATTACA GTCCATCTGC ATTCTAGCAG GAGATCACTG TCAGAGGGCC 
CAGGAATATG CAAGATCGAA ATTGGCAGAA TGTATGCCAC TTCCTTCCTT GGTGGTTTGT 
CCACCAACAT TAACAGGTCA TTGGGTAGAT GAAGTAGGTA AATTTTGTTC CAGAGAATAT 
CTCAATCCGT TGCATTATAC TGGCCCTCCC ACTGAAAGGA TAAGGTTGCA ACATCAAGTA 
AAAAGGCACA ATCTGATAGT AGCCTCATAT GATGTTGTGA GGAATGACAT AGACTTTTTT 
AGAAATATTA AATTTAACTA CTGTATTCTT GATGAAGGCC ATGTCATAAA AAATGGAAAA 
ACCAAGTTGT CCAAAGCAGT AAAGCAGCTG ACTGCTAATT ACAGGATTAT TCTTTCTGGA 
ACACCAATCC AGAACAATGT TTTGGAGTTG TGGTCATTAT TTGATTTCCT TATGCCTGGA 
TTTTTGGGTA CTGAACGCCA GTTTGCTGCT CGATATGGCA AACCTATATT AGCGAGTAGG 
GATGCACGAA GCTCCAGTCG AGAACAAGAA GCAGGTGTCC TGGCAATGGA CGCTCTGCAC 
CGCCAAGTCC TGCCGTTCCT TCTCAGAAGA ATGAAAGAAG ATGTTTTGCA GGATCTTCCA 
CCTAAAATTA TTCAAGACTA TTACTGTACT CTCAGCCCTC TCCAGGTTCA GCTTTATGAA 
GATTTTGCTA AGTCGCGTGC CAAGTGTGAT GTTGATGAGA CCGTGTCTTC AGCTGCACTT 
TCAGAAGAAA CTGAAAAACC AAAACTTAAA GCGACAGGTC ATGTATTCCA GGCGTTACAA 
TATTTACGTA AGCTCTGCAA CCATCCAGCA CTGGTCTTAA CCCCTCAGCA TCCAGAATTC 
AAGAGCACTA CTGAAAAACT GACCGTTCAG AATTCTTCTC TTCATGATAT TCAACACGCG 
CCTAAGCTCT CAGCCTTGAA ACAACTGCTG TTGGACTGTG GTTTGGGGAA TGGCAGTTCT 
ACAGAGAGTG GCACAGAGTC TGTTGTGGCA CAGCACAGAA TACTGATCTT CTGTCAGCTT 
AAAAGCATGC TTGATATAGT AGAACATGAT CTGCTCAAGC CTCACCTGCC CTCTGTCACT 
TACTTGAGAC TAGATGGCAG CATACCTCCT GGACAGCGGC ATTCCATTGT TTCCCGGTTT 
AACAACGACC CCTCCATCGA TGTTCTTCTA CTCACAACTC ACGTTGGTGG CCTGGGCCTG 
AACTTGACTG GAGCTGACAC TGTGGTGTTT GTGGAGCATG ACTGGAACCC TATGCGAGAT 
CTGCAGGCCA TGGACCGGGC CCATCGTATT GGGCAGAAAC GTGTGGTTAA TGTCTACCGG 
TTGATAACCA GAGGAACACT AGAAGAAAAG ATAATGGGCT TGCAGAAATT CAAGATGAAC 
ATCGCAAATA CTGTTATTAG CCAAGAAAAC TCTAGCTTAC AGAGTATGGG CACTGATCAA 
CTTCTTGACC TGTTCACTCT AGATAAGGAT GGAAAAGCAG AAAAGGCTGA CAGCTCCACT 
TCTGGGAAAG CAAGCATGAA ATCAGTTCTT GAGAACCTGA GTGACCTCTG GGACGCCGAG 
CAGTATGATT CGGAGTACAA CCTGGAGACC TTCATGCGCT CTCTCGAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Btaf1 ( XM_006526561.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006526624.1 Mus musculus B-TFIID TATA-box binding protein associated factor 1 (Btaf1), transcript variant X1, mRNA.

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CloneID	OMu55167
Clone ID Related Accession (Same CDS sequence)	XM_006526561.3 , XM_006526561.4
Accession Version	XM_006526561.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4941bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	TATA-binding protein-associated factor 172 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000085.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006526561.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTAATA GACAGAAGAA CAAAGCTAAA AGAATGGCCA AGTTATTTGC AAAACAGAGA 
TCCAGGGATG CAGTGGAAAC GAATGAGAAG AGCAATGACA GCACTGATGG AGAGCCAGAA 
GAAAAGAGAC GGAAAGTAGC AAATGTTGTT ATTAATCAGA CTGCAAGTGA TTCCAAAGTC 
TTGATAGATA ATGTTCCAGA AAGTTCTTCC TTAATTGAAG AGACAAATGA ATGGCCTTTG 
GAAAGCTTTT GTGAAGAACT TTGCAATGAC CTTTTTAATC CCTCCTGGGA GGTTCGACAT 
GGGGCAGGTA CTGGACTCAG AGAAATTCTT AAAGCTCATG GGAAAAGTGG TGGTAAAATG 
GGAGACAGCA CTTTAGAGGA GATGATTCAG CAGCATCAAG AGTGGTTGGA AGACTTAGTC 
ATTAGACTCC TTTGTGTTTT TGCGTTGGAC AGATTTGGAG ACTTCGTTTC TGATGAAGTT 
GTAGCGCCAG TTCGTGAGAC TTGTGCTCAA ACGTTAGGTG TGGTTTTAAA GCACATGAAC 
GAAACAGGAG TTCATAAGAC AGTGGATGTG CTGCTGAAAT TATTGACACA AGAACAGTGG 
GAAGTTAGAC ATGGTGGTCT TCTGGGAATA AAATATGCTT TGGCAGTTCG TCAGGATGTA 
ATTAATACTT TATTGCCTAA AGTCTTAACT CGAATAATCG AAGGACTCCA GGACCTTGAT 
GACGATGTTA GAGCTGTTGC TGCAGCATCC CTGGTTCCTG TAGTAGAAAG CCTTGTCTAC 
CTTCAGACAC AAAAGGTACC CTCCATTATA AATACCTTAT GGGATTCTCT TCTGGAATTA 
GATGATCTAA CAGCTTCAAC AAATAGTATT ATGACTCTCC TTTCATCCAT GTTAACTTAC 
CCTCAGGTCC AGCAATGCAG TATTCAACAG TCACTCACAG TTTTAGTCCC ACGTGTCTGG 
CCATTTTTGC ATCACACTAT ATCTTCAGTT CGAAGAGCAG CATTGGAAAC TCTCTTTACA 
TTATTATCAA CACAGGACGA GAACTCTTCA TCTTGGCTCA TACCTATCCT GTCGGATATG 
CTGAGACACA TTTTTCAGTT TTGTGTTTTA GAAAGCAGCC AGGAAATTCT GGATCTTATT 
CACAAGGTTT GGATGGAGCT TTTGAGCAAG GCGTCAGTTC AGTATTTGGT AGCAGCTGCA 
TGCCCATGGA TGAGTGCTTG GCTTTGCCTA ATGATGCAGC CTTCTCACTT ACCTATTGAT 
TTAAATATGC TGCTGGAAGT AAAAGCTAGA GCCAAGGAAA AAACAGGTGG TAAGGTACGG 
CAAGGTCAAA TCCAGAATAA AGAAGTGCTT CAGGAGTATA TTGCTGGTGC AGACACCATC 
ATGGAAGACC CAGCGACCAG AGATTTTGTA GTTATGAGGG CCAGGATGAT GGCAGCTAAG 
TTGTTAGGAG CACTCTGTTG CTATATTTGT GATCCAAGTG TAAATATGGT AAACCAGGAA 
ATTAAACCAG CGGATTCCCT TGGCCAGTTA CTGCTCTTCT ATTTGAATTC CAAATCTGCT 
TTACAGAGAA TTAGTGTTGC GTTAGTAATC TGTGAGTGGG CTGCTTTACA AAAGGAGTGT 
AAAGCTGTTA CCCTAGCTGT GCAACCGCGT TTACTTGATA TCCTTTCAGA ACATTTGTAT 
TATGATGAAA TTGCTGTTCC GTTCACACGT ATGCAGAATG AATGTAAACA GTTTATATCA 
TCATTAGCTG ATGCACATAT TGAAGTTGGT AATAGAGTAA ACAACAATGT TTTAACAATA 
GATCAAGCAA ACGACCTGGT AACCACTATT TTTAATGAAG TCACATCAAC TTTTGATTTA 
AATCCTCAAG TGTTACAGCA GTTAGATAGT AAACGGCATC AGGTCCAAAT GACAGTTGCA 
GAGACGAACC AGGAGTGGCA AGTACTGCAG CTGAGAGTAC ACACGTTTGC TGCGTGTGCT 
GTTGTGAGCT TGCAGCAGCT TCCGGAGAAA CTAAATCCTA TCATAAAACC ATTAATGGAA 
ACGATTAAAA AAGAAGAGAA TACGCTAGTG CAGAACTATG CTGCTCAGTA CATAGCTAAG 
CTTCTTCAGC AGTGTACAAC AAGGACACCA TGCCCTAATT CAAAAGTTAT TAAGAACCTC 
TGTAGCTCTC TTTGTGTGGA CCCATACCTC ACTCCCTGTG TTACGTGTCC AGTACCAACA 
CAAAGTGGCC AGGAAAATTC TAAAGGATCC AACTCAGAAA AGGATGGAAT GCACCATACT 
GTCACCAAGC ACAGGGGGAT CATTACACTC TACAGGCATC AGAAAGCAGC ATTTGCTATC 
ACCAGCAGGC GAGGTCCCAT CCCCAAAGCA ATCAAAGCTC AGATAGCAGA TCTACCTGCA 
GGAAGTAGTG GGACTATCCT GGTTGAACTT GATGAGGGTC AGAAGCCTTA CCTGGTACAG 
CGGAGAGGAG CTGAATTTGC CTTAACAACT ATAGTGAAGC ATTTTGGTGC TGAAATGGCA 
GTGAAGTTGC CACATCTTTG GGATGCTATG GTTGGCCCAT TGAAGTCTAT GATTGACCTA 
AATAATTTTG ATGGAAAGTC CCTCCTGGAA AGAGGTGATG TTCCTGCTCA AGAATTGGTG 
AATTCCCTGC AAGTGTTTGA AATAGCAGCA GCTTCCATGG ACTCTGCACT TCATCCCTTG 
CTGGTACAGC ACTTGCCTCA TCTTTATATG TGCCTTCAGT ACCCCAGCAC TGCAGTGAGG 
CACATGGCGG CTCGTTGCAT AGGTGTCATG AGTAAAATAG CTACCATGGA AACAATGAAT 
ATTTTTTTGG AGAAGGTACT TCCTTGGTTG GGAGCAATTG ATGACAATGT CAAGCAAGAA 
GGTGCAATTG AAGCACTTGC CTGTGTTATG GAACAATTGG ATGTCGGTAT TGTTCCATAT 
ATTGTCCTTT TAGTTGTTCC TGTATTGGGA AGGATGAGTG ATCAGACAGA CAGTGTGAGA 
TTCATGGCTA CTCAGTGCTT TGCAACACTG ATTAGACTGA TGCCACTTGA GGCAGGCATT 
CCAGACCCTC CAAACATGTC AGAAGAATTA ATCCAGTTGA AAGCAAAAGA GCGACACTTT 
TTAGAGCAAT TGTTAGATGG GAAGAAGTTA GAAAATTACA AGATCCCAGT GCCAATCAAT 
GCTGAACTCA GGAAATATCA GCAGGATGGT GTGAACTGGT TAGCCTTTCT CAATAAGTAT 
AAGCTTCATG GAATTCTGTG TGATGACATG GGCTTAGGAA AAACATTACA GTCCATCTGC 
ATTCTAGCAG GAGATCACTG TCAGAGGGCC CAGGAATATG CAAGATCGAA ATTGGCAGAA 
TGTATGCCAC TTCCTTCCTT GGTGGTTTGT CCACCAACAT TAACAGGTCA TTGGGTAGAT 
GAAGTAGGTA AATTTTGTTC CAGAGAATAT CTCAATCCGT TGCATTATAC TGGCCCTCCC 
ACTGAAAGGA TAAGGTTGCA ACATCAAGTA AAAAGGCACA ATCTGATAGT AGCCTCATAT 
GATGTTGTGA GGAATGACAT AGACTTTTTT AGAAATATTA AATTTAACTA CTGTATTCTT 
GATGAAGGCC ATGTCATAAA AAATGGAAAA ACCAAGTTGT CCAAAGCAGT AAAGCAGCTG 
ACTGCTAATT ACAGGATTAT TCTTTCTGGA ACACCAATCC AGAACAATGT TTTGGAGTTG 
TGGTCATTAT TTGATTTCCT TATGCCTGGA TTTTTGGGTA CTGAACGCCA GTTTGCTGCT 
CGATATGGCA AACCTATATT AGCGAGTAGG GATGCACGAA GCTCCAGTCG AGAACAAGAA 
GCAGGTGTCC TGGCAATGGA CGCTCTGCAC CGCCAAGTCC TGCCGTTCCT TCTCAGAAGA 
ATGAAAGAAG ATGTTTTGCA GGATCTTCCA CCTAAAATTA TTCAAGACTA TTACTGTACT 
CTCAGCCCTC TCCAGGTTCA GCTTTATGAA GATTTTGCTA AGTCGCGTGC CAAGTGTGAT 
GTTGATGAGA CCGTGTCTTC AGCTGCACTT TCAGAAGAAA CTGAAAAACC AAAACTTAAA 
GCGACAGGTC ATGTATTCCA GGCGTTACAA TATTTACGTA AGCTCTGCAA CCATCCAGCA 
CTGGTCTTAA CCCCTCAGCA TCCAGAATTC AAGAGCACTA CTGAAAAACT GACCGTTCAG 
AATTCTTCTC TTCATGATAT TCAACACGCG CCTAAGCTCT CAGCCTTGAA ACAACTGCTG 
TTGGACTGTG GTTTGGGGAA TGGCAGTTCT ACAGAGAGTG GCACAGAGTC TGTTGTGGCA 
CAGCACAGAA TACTGATCTT CTGTCAGCTT AAAAGCATGC TTGATATAGT AGAACATGAT 
CTGCTCAAGC CTCACCTGCC CTCTGTCACT TACTTGAGAC TAGATGGCAG CATACCTCCT 
GGACAGCGGC ATTCCATTGT TTCCCGGTTT AACAACGACC CCTCCATCGA TGTTCTTCTA 
CTCACAACTC ACGTTGGTGG CCTGGGCCTG AACTTGACTG GAGCTGACAC TGTGGTGTTT 
GTGGAGCATG ACTGGAACCC TATGCGAGAT CTGCAGGCCA TGGACCGGGC CCATCGTATT 
GGGCAGAAAC GTGTGGTTAA TGTCTACCGG TTGATAACCA GAGGAACACT AGAAGAAAAG 
ATAATGGGCT TGCAGAAATT CAAGATGAAC ATCGCAAATA CTGTTATTAG CCAAGAAAAC 
TCTAGCTTAC AGAGTATGGG CACTGATCAA CTTCTTGACC TGTTCACTCT AGATAAGGAT 
GGAAAAGCAG AAAAGGCTGA CAGCTCCACT TCTGGGAAAG CAAGCATGAA ATCAGTTCTT 
GAGAACCTGA GTGACCTCTG GGACGCCGAG CAGTATGATT CGGAGTACAA CCTGGAGACC 
TTCATGCGCT CTCTCGAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006526624.1
CDS	117..5057
Translation	MSNRQKNKAKRMAKLFAKQRSRDAVETNEKSNDSTDGEPEEKRRKVANVVINQTASDSKVLIDNVPESSSLIEETNEWPLESFCEELCNDLFNPSWEVRHGAGTGLREILKAHGKSGGKMGDSTLEEMIQQHQEWLEDLVIRLLCVFALDRFGDFVSDEVVAPVRETCAQTLGVVLKHMNETGVHKTVDVLLKLLTQEQWEVRHGGLLGIKYALAVRQDVINTLLPKVLTRIIEGLQDLDDDVRAVAAASLVPVVESLVYLQTQKVPSIINTLWDSLLELDDLTASTNSIMTLLSSMLTYPQVQQCSIQQSLTVLVPRVWPFLHHTISSVRRAALETLFTLLSTQDENSSSWLIPILSDMLRHIFQFCVLESSQEILDLIHKVWMELLSKASVQYLVAAACPWMSAWLCLMMQPSHLPIDLNMLLEVKARAKEKTGGKVRQGQIQNKEVLQEYIAGADTIMEDPATRDFVVMRARMMAAKLLGALCCYICDPSVNMVNQEIKPADSLGQLLLFYLNSKSALQRISVALVICEWAALQKECKAVTLAVQPRLLDILSEHLYYDEIAVPFTRMQNECKQFISSLADAHIEVGNRVNNNVLTIDQANDLVTTIFNEVTSTFDLNPQVLQQLDSKRHQVQMTVAETNQEWQVLQLRVHTFAACAVVSLQQLPEKLNPIIKPLMETIKKEENTLVQNYAAQYIAKLLQQCTTRTPCPNSKVIKNLCSSLCVDPYLTPCVTCPVPTQSGQENSKGSNSEKDGMHHTVTKHRGIITLYRHQKAAFAITSRRGPIPKAIKAQIADLPAGSSGTILVELDEGQKPYLVQRRGAEFALTTIVKHFGAEMAVKLPHLWDAMVGPLKSMIDLNNFDGKSLLERGDVPAQELVNSLQVFEIAAASMDSALHPLLVQHLPHLYMCLQYPSTAVRHMAARCIGVMSKIATMETMNIFLEKVLPWLGAIDDNVKQEGAIEALACVMEQLDVGIVPYIVLLVVPVLGRMSDQTDSVRFMATQCFATLIRLMPLEAGIPDPPNMSEELIQLKAKERHFLEQLLDGKKLENYKIPVPINAELRKYQQDGVNWLAFLNKYKLHGILCDDMGLGKTLQSICILAGDHCQRAQEYARSKLAECMPLPSLVVCPPTLTGHWVDEVGKFCSREYLNPLHYTGPPTERIRLQHQVKRHNLIVASYDVVRNDIDFFRNIKFNYCILDEGHVIKNGKTKLSKAVKQLTANYRIILSGTPIQNNVLELWSLFDFLMPGFLGTERQFAARYGKPILASRDARSSSREQEAGVLAMDALHRQVLPFLLRRMKEDVLQDLPPKIIQDYYCTLSPLQVQLYEDFAKSRAKCDVDETVSSAALSEETEKPKLKATGHVFQALQYLRKLCNHPALVLTPQHPEFKSTTEKLTVQNSSLHDIQHAPKLSALKQLLLDCGLGNGSSTESGTESVVAQHRILIFCQLKSMLDIVEHDLLKPHLPSVTYLRLDGSIPPGQRHSIVSRFNNDPSIDVLLLTTHVGGLGLNLTGADTVVFVEHDWNPMRDLQAMDRAHRIGQKRVVNVYRLITRGTLEEKIMGLQKFKMNIANTVISQENSSLQSMGTDQLLDLFTLDKDGKAEKADSSTSGKASMKSVLENLSDLWDAEQYDSEYNLETFMRSLE

Target ORF information:

RefSeq Version	XM_006526561.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus B-TFIID TATA-box binding protein associated factor 1 (Btaf1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006526561.4

ORF Insert Sequence:

ATGAGTAATA GACAGAAGAA CAAAGCTAAA AGAATGGCCA AGTTATTTGC AAAACAGAGA 
TCCAGGGATG CAGTGGAAAC GAATGAGAAG AGCAATGACA GCACTGATGG AGAGCCAGAA 
GAAAAGAGAC GGAAAGTAGC AAATGTTGTT ATTAATCAGA CTGCAAGTGA TTCCAAAGTC 
TTGATAGATA ATGTTCCAGA AAGTTCTTCC TTAATTGAAG AGACAAATGA ATGGCCTTTG 
GAAAGCTTTT GTGAAGAACT TTGCAATGAC CTTTTTAATC CCTCCTGGGA GGTTCGACAT 
GGGGCAGGTA CTGGACTCAG AGAAATTCTT AAAGCTCATG GGAAAAGTGG TGGTAAAATG 
GGAGACAGCA CTTTAGAGGA GATGATTCAG CAGCATCAAG AGTGGTTGGA AGACTTAGTC 
ATTAGACTCC TTTGTGTTTT TGCGTTGGAC AGATTTGGAG ACTTCGTTTC TGATGAAGTT 
GTAGCGCCAG TTCGTGAGAC TTGTGCTCAA ACGTTAGGTG TGGTTTTAAA GCACATGAAC 
GAAACAGGAG TTCATAAGAC AGTGGATGTG CTGCTGAAAT TATTGACACA AGAACAGTGG 
GAAGTTAGAC ATGGTGGTCT TCTGGGAATA AAATATGCTT TGGCAGTTCG TCAGGATGTA 
ATTAATACTT TATTGCCTAA AGTCTTAACT CGAATAATCG AAGGACTCCA GGACCTTGAT 
GACGATGTTA GAGCTGTTGC TGCAGCATCC CTGGTTCCTG TAGTAGAAAG CCTTGTCTAC 
CTTCAGACAC AAAAGGTACC CTCCATTATA AATACCTTAT GGGATTCTCT TCTGGAATTA 
GATGATCTAA CAGCTTCAAC AAATAGTATT ATGACTCTCC TTTCATCCAT GTTAACTTAC 
CCTCAGGTCC AGCAATGCAG TATTCAACAG TCACTCACAG TTTTAGTCCC ACGTGTCTGG 
CCATTTTTGC ATCACACTAT ATCTTCAGTT CGAAGAGCAG CATTGGAAAC TCTCTTTACA 
TTATTATCAA CACAGGACGA GAACTCTTCA TCTTGGCTCA TACCTATCCT GTCGGATATG 
CTGAGACACA TTTTTCAGTT TTGTGTTTTA GAAAGCAGCC AGGAAATTCT GGATCTTATT 
CACAAGGTTT GGATGGAGCT TTTGAGCAAG GCGTCAGTTC AGTATTTGGT AGCAGCTGCA 
TGCCCATGGA TGAGTGCTTG GCTTTGCCTA ATGATGCAGC CTTCTCACTT ACCTATTGAT 
TTAAATATGC TGCTGGAAGT AAAAGCTAGA GCCAAGGAAA AAACAGGTGG TAAGGTACGG 
CAAGGTCAAA TCCAGAATAA AGAAGTGCTT CAGGAGTATA TTGCTGGTGC AGACACCATC 
ATGGAAGACC CAGCGACCAG AGATTTTGTA GTTATGAGGG CCAGGATGAT GGCAGCTAAG 
TTGTTAGGAG CACTCTGTTG CTATATTTGT GATCCAAGTG TAAATATGGT AAACCAGGAA 
ATTAAACCAG CGGATTCCCT TGGCCAGTTA CTGCTCTTCT ATTTGAATTC CAAATCTGCT 
TTACAGAGAA TTAGTGTTGC GTTAGTAATC TGTGAGTGGG CTGCTTTACA AAAGGAGTGT 
AAAGCTGTTA CCCTAGCTGT GCAACCGCGT TTACTTGATA TCCTTTCAGA ACATTTGTAT 
TATGATGAAA TTGCTGTTCC GTTCACACGT ATGCAGAATG AATGTAAACA GTTTATATCA 
TCATTAGCTG ATGCACATAT TGAAGTTGGT AATAGAGTAA ACAACAATGT TTTAACAATA 
GATCAAGCAA ACGACCTGGT AACCACTATT TTTAATGAAG TCACATCAAC TTTTGATTTA 
AATCCTCAAG TGTTACAGCA GTTAGATAGT AAACGGCATC AGGTCCAAAT GACAGTTGCA 
GAGACGAACC AGGAGTGGCA AGTACTGCAG CTGAGAGTAC ACACGTTTGC TGCGTGTGCT 
GTTGTGAGCT TGCAGCAGCT TCCGGAGAAA CTAAATCCTA TCATAAAACC ATTAATGGAA 
ACGATTAAAA AAGAAGAGAA TACGCTAGTG CAGAACTATG CTGCTCAGTA CATAGCTAAG 
CTTCTTCAGC AGTGTACAAC AAGGACACCA TGCCCTAATT CAAAAGTTAT TAAGAACCTC 
TGTAGCTCTC TTTGTGTGGA CCCATACCTC ACTCCCTGTG TTACGTGTCC AGTACCAACA 
CAAAGTGGCC AGGAAAATTC TAAAGGATCC AACTCAGAAA AGGATGGAAT GCACCATACT 
GTCACCAAGC ACAGGGGGAT CATTACACTC TACAGGCATC AGAAAGCAGC ATTTGCTATC 
ACCAGCAGGC GAGGTCCCAT CCCCAAAGCA ATCAAAGCTC AGATAGCAGA TCTACCTGCA 
GGAAGTAGTG GGACTATCCT GGTTGAACTT GATGAGGGTC AGAAGCCTTA CCTGGTACAG 
CGGAGAGGAG CTGAATTTGC CTTAACAACT ATAGTGAAGC ATTTTGGTGC TGAAATGGCA 
GTGAAGTTGC CACATCTTTG GGATGCTATG GTTGGCCCAT TGAAGTCTAT GATTGACCTA 
AATAATTTTG ATGGAAAGTC CCTCCTGGAA AGAGGTGATG TTCCTGCTCA AGAATTGGTG 
AATTCCCTGC AAGTGTTTGA AATAGCAGCA GCTTCCATGG ACTCTGCACT TCATCCCTTG 
CTGGTACAGC ACTTGCCTCA TCTTTATATG TGCCTTCAGT ACCCCAGCAC TGCAGTGAGG 
CACATGGCGG CTCGTTGCAT AGGTGTCATG AGTAAAATAG CTACCATGGA AACAATGAAT 
ATTTTTTTGG AGAAGGTACT TCCTTGGTTG GGAGCAATTG ATGACAATGT CAAGCAAGAA 
GGTGCAATTG AAGCACTTGC CTGTGTTATG GAACAATTGG ATGTCGGTAT TGTTCCATAT 
ATTGTCCTTT TAGTTGTTCC TGTATTGGGA AGGATGAGTG ATCAGACAGA CAGTGTGAGA 
TTCATGGCTA CTCAGTGCTT TGCAACACTG ATTAGACTGA TGCCACTTGA GGCAGGCATT 
CCAGACCCTC CAAACATGTC AGAAGAATTA ATCCAGTTGA AAGCAAAAGA GCGACACTTT 
TTAGAGCAAT TGTTAGATGG GAAGAAGTTA GAAAATTACA AGATCCCAGT GCCAATCAAT 
GCTGAACTCA GGAAATATCA GCAGGATGGT GTGAACTGGT TAGCCTTTCT CAATAAGTAT 
AAGCTTCATG GAATTCTGTG TGATGACATG GGCTTAGGAA AAACATTACA GTCCATCTGC 
ATTCTAGCAG GAGATCACTG TCAGAGGGCC CAGGAATATG CAAGATCGAA ATTGGCAGAA 
TGTATGCCAC TTCCTTCCTT GGTGGTTTGT CCACCAACAT TAACAGGTCA TTGGGTAGAT 
GAAGTAGGTA AATTTTGTTC CAGAGAATAT CTCAATCCGT TGCATTATAC TGGCCCTCCC 
ACTGAAAGGA TAAGGTTGCA ACATCAAGTA AAAAGGCACA ATCTGATAGT AGCCTCATAT 
GATGTTGTGA GGAATGACAT AGACTTTTTT AGAAATATTA AATTTAACTA CTGTATTCTT 
GATGAAGGCC ATGTCATAAA AAATGGAAAA ACCAAGTTGT CCAAAGCAGT AAAGCAGCTG 
ACTGCTAATT ACAGGATTAT TCTTTCTGGA ACACCAATCC AGAACAATGT TTTGGAGTTG 
TGGTCATTAT TTGATTTCCT TATGCCTGGA TTTTTGGGTA CTGAACGCCA GTTTGCTGCT 
CGATATGGCA AACCTATATT AGCGAGTAGG GATGCACGAA GCTCCAGTCG AGAACAAGAA 
GCAGGTGTCC TGGCAATGGA CGCTCTGCAC CGCCAAGTCC TGCCGTTCCT TCTCAGAAGA 
ATGAAAGAAG ATGTTTTGCA GGATCTTCCA CCTAAAATTA TTCAAGACTA TTACTGTACT 
CTCAGCCCTC TCCAGGTTCA GCTTTATGAA GATTTTGCTA AGTCGCGTGC CAAGTGTGAT 
GTTGATGAGA CCGTGTCTTC AGCTGCACTT TCAGAAGAAA CTGAAAAACC AAAACTTAAA 
GCGACAGGTC ATGTATTCCA GGCGTTACAA TATTTACGTA AGCTCTGCAA CCATCCAGCA 
CTGGTCTTAA CCCCTCAGCA TCCAGAATTC AAGAGCACTA CTGAAAAACT GACCGTTCAG 
AATTCTTCTC TTCATGATAT TCAACACGCG CCTAAGCTCT CAGCCTTGAA ACAACTGCTG 
TTGGACTGTG GTTTGGGGAA TGGCAGTTCT ACAGAGAGTG GCACAGAGTC TGTTGTGGCA 
CAGCACAGAA TACTGATCTT CTGTCAGCTT AAAAGCATGC TTGATATAGT AGAACATGAT 
CTGCTCAAGC CTCACCTGCC CTCTGTCACT TACTTGAGAC TAGATGGCAG CATACCTCCT 
GGACAGCGGC ATTCCATTGT TTCCCGGTTT AACAACGACC CCTCCATCGA TGTTCTTCTA 
CTCACAACTC ACGTTGGTGG CCTGGGCCTG AACTTGACTG GAGCTGACAC TGTGGTGTTT 
GTGGAGCATG ACTGGAACCC TATGCGAGAT CTGCAGGCCA TGGACCGGGC CCATCGTATT 
GGGCAGAAAC GTGTGGTTAA TGTCTACCGG TTGATAACCA GAGGAACACT AGAAGAAAAG 
ATAATGGGCT TGCAGAAATT CAAGATGAAC ATCGCAAATA CTGTTATTAG CCAAGAAAAC 
TCTAGCTTAC AGAGTATGGG CACTGATCAA CTTCTTGACC TGTTCACTCT AGATAAGGAT 
GGAAAAGCAG AAAAGGCTGA CAGCTCCACT TCTGGGAAAG CAAGCATGAA ATCAGTTCTT 
GAGAACCTGA GTGACCTCTG GGACGCCGAG CAGTATGATT CGGAGTACAA CCTGGAGACC 
TTCATGCGCT CTCTCGAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Btaf1 ( NM_001080706.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001074175.1 Mus musculus B-TFIID TATA-box binding protein associated factor 1 (Btaf1), mRNA.

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CloneID	OMu01417
Clone ID Related Accession (Same CDS sequence)	NM_001080706.1
Accession Version	NM_001080706.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5547bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-25
Organism	Mus musculus(house mouse)
Product	TATA-binding protein-associated factor 172
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AC118931.8. On Jan 21, 2007 this sequence version replaced XM_129248.8. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: AB287295.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849384, SAMN00849387 [ECO:0000348] ##Evidence-Data-END##

ATGGCGGTCT CCAGGCTTGA TCGTCTTTTT ATCTTACTGG ATACTGGTAC TACTCCAGTT 
ACAAGAAAGG CTGCTGCACA GCAACTTGGA GAAGTGGTGA AGCTTCATCC TCATGAATTA 
AATAATTTGT TATCTAAGGT ATTAATATAT TTAAGGAGTA CAAATTGGGA TACCCGGATT 
GCAGCAGGAC AAGCTGTTGA AGCTATAGTG AAAAACGTGC CTGAGTGGAA TCCAGTGCCA 
AGAACCAAAC AAGAACCTAC AGAGAGTTCT ATGGAAGATT CATCTACTAC AGATCGATTA 
AATTTTGACA GATTTGATAT ATGCAGATTG TTACAGCATG GGGCATCACT TTTGGGGTCT 
GCTGGTGCAG AATTTGAAGT CCAAGATGAA AAATCAGGTG AAGTGGATCC TAAAGAGAGG 
ATAGCACGCC AACGGAAATT ATTACAGAAG AAACTTGGAC TTAATATGGG AGAAGCAATT 
GGAATGAGTA CTGAAGAACT CTTCAACGAT GAAGATTTGG ATTATACCCC AACTTCAGCA 
GCCCTTGTAA ACAAACAATC TACTCTCCAA GCAGCTGAAT TGATTGATTC AGAATTCCGA 
GCAGGAATGA GTAATAGACA GAAGAACAAA GCTAAAAGAA TGGCCAAGTT ATTTGCAAAA 
CAGAGATCCA GGGATGCAGT GGAAACGAAT GAGAAGAGCA ATGACAGCAC TGATGGAGAG 
CCAGAAGAAA AGAGACGGAA AGTAGCAAAT GTTGTTATTA ATCAGACTGC AAGTGATTCC 
AAAGTCTTGA TAGATAATGT TCCAGAAAGT TCTTCCTTAA TTGAAGAGAC AAATGAATGG 
CCTTTGGAAA GCTTTTGTGA AGAACTTTGC AATGACCTTT TTAATCCCTC CTGGGAGGTT 
CGACATGGGG CAGGTACTGG ACTCAGAGAA ATTCTTAAAG CTCATGGGAA AAGTGGTGGT 
AAAATGGGAG ACAGCACTTT AGAGGAGATG ATTCAGCAGC ATCAAGAGTG GTTGGAAGAC 
TTAGTCATTA GACTCCTTTG TGTTTTTGCG TTGGACAGAT TTGGAGACTT CGTTTCTGAT 
GAAGTTGTAG CGCCAGTTCG TGAGACTTGT GCTCAAACGT TAGGTGTGGT TTTAAAGCAC 
ATGAACGAAA CAGGAGTTCA TAAGACAGTG GATGTGCTGC TGAAATTATT GACACAAGAA 
CAGTGGGAAG TTAGACATGG TGGTCTTCTG GGAATAAAAT ATGCTTTGGC AGTTCGTCAG 
GATGTAATTA ATACTTTATT GCCTAAAGTC TTAACTCGAA TAATCGAAGG ACTCCAGGAC 
CTTGATGACG ATGTTAGAGC TGTTGCTGCA GCATCCCTGG TTCCTGTAGT AGAAAGCCTT 
GTCTACCTTC AGACACAAAA GGTACCCTCC ATTATAAATA CCTTATGGGA TTCTCTTCTG 
GAATTAGATG ATCTAACAGC TTCAACAAAT AGTATTATGA CTCTCCTTTC ATCCATGTTA 
ACTTACCCTC AGGTCCAGCA ATGCAGTATT CAACAGTCAC TCACAGTTTT AGTCCCACGT 
GTCTGGCCAT TTTTGCATCA CACTATATCT TCAGTTCGAA GAGCAGCATT GGAAACTCTC 
TTTACATTAT TATCAACACA GGACGAGAAC TCTTCATCTT GGCTCATACC TATCCTGTCG 
GATATGCTGA GACACATTTT TCAGTTTTGT GTTTTAGAAA GCAGCCAGGA AATTCTGGAT 
CTTATTCACA AGGTTTGGAT GGAGCTTTTG AGCAAGGCGT CAGTTCAGTA TTTGGTAGCA 
GCTGCATGCC CATGGATGAG TGCTTGGCTT TGCCTAATGA TGCAGCCTTC TCACTTACCT 
ATTGATTTAA ATATGCTGCT GGAAGTAAAA GCTAGAGCCA AGGAAAAAAC AGGTGGTAAG 
GTACGGCAAG GTCAAATCCA GAATAAAGAA GTGCTTCAGG AGTATATTGC TGGTGCAGAC 
ACCATCATGG AAGACCCAGC GACCAGAGAT TTTGTAGTTA TGAGGGCCAG GATGATGGCA 
GCTAAGTTGT TAGGAGCACT CTGTTGCTAT ATTTGTGATC CAAGTGTAAA TATGGTAAAC 
CAGGAAATTA AACCAGCGGA TTCCCTTGGC CAGTTACTGC TCTTCTATTT GAATTCCAAA 
TCTGCTTTAC AGAGAATTAG TGTTGCGTTA GTAATCTGTG AGTGGGCTGC TTTACAAAAG 
GAGTGTAAAG CTGTTACCCT AGCTGTGCAA CCGCGTTTAC TTGATATCCT TTCAGAACAT 
TTGTATTATG ATGAAATTGC TGTTCCGTTC ACACGTATGC AGAATGAATG TAAACAGTTT 
ATATCATCAT TAGCTGATGC ACATATTGAA GTTGGTAATA GAGTAAACAA CAATGTTTTA 
ACAATAGATC AAGCAAACGA CCTGGTAACC ACTATTTTTA ATGAAGTCAC ATCAACTTTT 
GATTTAAATC CTCAAGTGTT ACAGCAGTTA GATAGTAAAC GGCATCAGGT CCAAATGACA 
GTTGCAGAGA CGAACCAGGA GTGGCAAGTA CTGCAGCTGA GAGTACACAC GTTTGCTGCG 
TGTGCTGTTG TGAGCTTGCA GCAGCTTCCG GAGAAACTAA ATCCTATCAT AAAACCATTA 
ATGGAAACGA TTAAAAAAGA AGAGAATACG CTAGTGCAGA ACTATGCTGC TCAGTACATA 
GCTAAGCTTC TTCAGCAGTG TACAACAAGG ACACCATGCC CTAATTCAAA AGTTATTAAG 
AACCTCTGTA GCTCTCTTTG TGTGGACCCA TACCTCACTC CCTGTGTTAC GTGTCCAGTA 
CCAACACAAA GTGGCCAGGA AAATTCTAAA GGATCCAACT CAGAAAAGGA TGGAATGCAC 
CATACTGTCA CCAAGCACAG GGGGATCATT ACACTCTACA GGCATCAGAA AGCAGCATTT 
GCTATCACCA GCAGGCGAGG TCCCATCCCC AAAGCAATCA AAGCTCAGAT AGCAGATCTA 
CCTGCAGGAA GTAGTGGGAC TATCCTGGTT GAACTTGATG AGGGTCAGAA GCCTTACCTG 
GTACAGCGGA GAGGAGCTGA ATTTGCCTTA ACAACTATAG TGAAGCATTT TGGTGCTGAA 
ATGGCAGTGA AGTTGCCACA TCTTTGGGAT GCTATGGTTG GCCCATTGAA GTCTATGATT 
GACCTAAATA ATTTTGATGG AAAGTCCCTC CTGGAAAGAG GTGATGTTCC TGCTCAAGAA 
TTGGTGAATT CCCTGCAAGT GTTTGAAATA GCAGCAGCTT CCATGGACTC TGCACTTCAT 
CCCTTGCTGG TACAGCACTT GCCTCATCTT TATATGTGCC TTCAGTACCC CAGCACTGCA 
GTGAGGCACA TGGCGGCTCG TTGCATAGGT GTCATGAGTA AAATAGCTAC CATGGAAACA 
ATGAATATTT TTTTGGAGAA GGTACTTCCT TGGTTGGGAG CAATTGATGA CAATGTCAAG 
CAAGAAGGTG CAATTGAAGC ACTTGCCTGT GTTATGGAAC AATTGGATGT CGGTATTGTT 
CCATATATTG TCCTTTTAGT TGTTCCTGTA TTGGGAAGGA TGAGTGATCA GACAGACAGT 
GTGAGATTCA TGGCTACTCA GTGCTTTGCA ACACTGATTA GACTGATGCC ACTTGAGGCA 
GGCATTCCAG ACCCTCCAAA CATGTCAGAA GAATTAATCC AGTTGAAAGC AAAAGAGCGA 
CACTTTTTAG AGCAATTGTT AGATGGGAAG AAGTTAGAAA ATTACAAGAT CCCAGTGCCA 
ATCAATGCTG AACTCAGGAA ATATCAGCAG GATGGTGTGA ACTGGTTAGC CTTTCTCAAT 
AAGTATAAGC TTCATGGAAT TCTGTGTGAT GACATGGGCT TAGGAAAAAC ATTACAGTCC 
ATCTGCATTC TAGCAGGAGA TCACTGTCAG AGGGCCCAGG AATATGCAAG ATCGAAATTG 
GCAGAATGTA TGCCACTTCC TTCCTTGGTG GTTTGTCCAC CAACATTAAC AGGTCATTGG 
GTAGATGAAG TAGGTAAATT TTGTTCCAGA GAATATCTCA ATCCGTTGCA TTATACTGGC 
CCTCCCACTG AAAGGATAAG GTTGCAACAT CAAGTAAAAA GGCACAATCT GATAGTAGCC 
TCATATGATG TTGTGAGGAA TGACATAGAC TTTTTTAGAA ATATTAAATT TAACTACTGT 
ATTCTTGATG AAGGCCATGT CATAAAAAAT GGAAAAACCA AGTTGTCCAA AGCAGTAAAG 
CAGCTGACTG CTAATTACAG GATTATTCTT TCTGGAACAC CAATCCAGAA CAATGTTTTG 
GAGTTGTGGT CATTATTTGA TTTCCTTATG CCTGGATTTT TGGGTACTGA ACGCCAGTTT 
GCTGCTCGAT ATGGCAAACC TATATTAGCG AGTAGGGATG CACGAAGCTC CAGTCGAGAA 
CAAGAAGCAG GTGTCCTGGC AATGGACGCT CTGCACCGCC AAGTCCTGCC GTTCCTTCTC 
AGAAGAATGA AAGAAGATGT TTTGCAGGAT CTTCCACCTA AAATTATTCA AGACTATTAC 
TGTACTCTCA GCCCTCTCCA GGTTCAGCTT TATGAAGATT TTGCTAAGTC GCGTGCCAAG 
TGTGATGTTG ATGAGACCGT GTCTTCAGCT GCACTTTCAG AAGAAACTGA AAAACCAAAA 
CTTAAAGCGA CAGGTCATGT ATTCCAGGCG TTACAATATT TACGTAAGCT CTGCAACCAT 
CCAGCACTGG TCTTAACCCC TCAGCATCCA GAATTCAAGA GCACTACTGA AAAACTGACC 
GTTCAGAATT CTTCTCTTCA TGATATTCAA CACGCGCCTA AGCTCTCAGC CTTGAAACAA 
CTGCTGTTGG ACTGTGGTTT GGGGAATGGC AGTTCTACAG AGAGTGGCAC AGAGTCTGTT 
GTGGCACAGC ACAGAATACT GATCTTCTGT CAGCTTAAAA GCATGCTTGA TATAGTAGAA 
CATGATCTGC TCAAGCCTCA CCTGCCCTCT GTCACTTACT TGAGACTAGA TGGCAGCATA 
CCTCCTGGAC AGCGGCATTC CATTGTTTCC CGGTTTAACA ACGACCCCTC CATCGATGTT 
CTTCTACTCA CAACTCACGT TGGTGGCCTG GGCCTGAACT TGACTGGAGC TGACACTGTG 
GTGTTTGTGG AGCATGACTG GAACCCTATG CGAGATCTGC AGGCCATGGA CCGGGCCCAT 
CGTATTGGGC AGAAACGTGT GGTTAATGTC TACCGGTTGA TAACCAGAGG AACACTAGAA 
GAAAAGATAA TGGGCTTGCA GAAATTCAAG ATGAACATCG CAAATACTGT TATTAGCCAA 
GAAAACTCTA GCTTACAGAG TATGGGCACT GATCAACTTC TTGACCTGTT CACTCTAGAT 
AAGGATGGAA AAGCAGAAAA GGCTGACAGC TCCACTTCTG GGAAAGCAAG CATGAAATCA 
GTTCTTGAGA ACCTGAGTGA CCTCTGGGAC GCCGAGCAGT ATGATTCGGA GTACAACCTG 
GAGACCTTCA TGCGCTCTCT CGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001074175.1
CDS	310..5856
Translation	MAVSRLDRLFILLDTGTTPVTRKAAAQQLGEVVKLHPHELNNLLSKVLIYLRSTNWDTRIAAGQAVEAIVKNVPEWNPVPRTKQEPTESSMEDSSTTDRLNFDRFDICRLLQHGASLLGSAGAEFEVQDEKSGEVDPKERIARQRKLLQKKLGLNMGEAIGMSTEELFNDEDLDYTPTSAALVNKQSTLQAAELIDSEFRAGMSNRQKNKAKRMAKLFAKQRSRDAVETNEKSNDSTDGEPEEKRRKVANVVINQTASDSKVLIDNVPESSSLIEETNEWPLESFCEELCNDLFNPSWEVRHGAGTGLREILKAHGKSGGKMGDSTLEEMIQQHQEWLEDLVIRLLCVFALDRFGDFVSDEVVAPVRETCAQTLGVVLKHMNETGVHKTVDVLLKLLTQEQWEVRHGGLLGIKYALAVRQDVINTLLPKVLTRIIEGLQDLDDDVRAVAAASLVPVVESLVYLQTQKVPSIINTLWDSLLELDDLTASTNSIMTLLSSMLTYPQVQQCSIQQSLTVLVPRVWPFLHHTISSVRRAALETLFTLLSTQDENSSSWLIPILSDMLRHIFQFCVLESSQEILDLIHKVWMELLSKASVQYLVAAACPWMSAWLCLMMQPSHLPIDLNMLLEVKARAKEKTGGKVRQGQIQNKEVLQEYIAGADTIMEDPATRDFVVMRARMMAAKLLGALCCYICDPSVNMVNQEIKPADSLGQLLLFYLNSKSALQRISVALVICEWAALQKECKAVTLAVQPRLLDILSEHLYYDEIAVPFTRMQNECKQFISSLADAHIEVGNRVNNNVLTIDQANDLVTTIFNEVTSTFDLNPQVLQQLDSKRHQVQMTVAETNQEWQVLQLRVHTFAACAVVSLQQLPEKLNPIIKPLMETIKKEENTLVQNYAAQYIAKLLQQCTTRTPCPNSKVIKNLCSSLCVDPYLTPCVTCPVPTQSGQENSKGSNSEKDGMHHTVTKHRGIITLYRHQKAAFAITSRRGPIPKAIKAQIADLPAGSSGTILVELDEGQKPYLVQRRGAEFALTTIVKHFGAEMAVKLPHLWDAMVGPLKSMIDLNNFDGKSLLERGDVPAQELVNSLQVFEIAAASMDSALHPLLVQHLPHLYMCLQYPSTAVRHMAARCIGVMSKIATMETMNIFLEKVLPWLGAIDDNVKQEGAIEALACVMEQLDVGIVPYIVLLVVPVLGRMSDQTDSVRFMATQCFATLIRLMPLEAGIPDPPNMSEELIQLKAKERHFLEQLLDGKKLENYKIPVPINAELRKYQQDGVNWLAFLNKYKLHGILCDDMGLGKTLQSICILAGDHCQRAQEYARSKLAECMPLPSLVVCPPTLTGHWVDEVGKFCSREYLNPLHYTGPPTERIRLQHQVKRHNLIVASYDVVRNDIDFFRNIKFNYCILDEGHVIKNGKTKLSKAVKQLTANYRIILSGTPIQNNVLELWSLFDFLMPGFLGTERQFAARYGKPILASRDARSSSREQEAGVLAMDALHRQVLPFLLRRMKEDVLQDLPPKIIQDYYCTLSPLQVQLYEDFAKSRAKCDVDETVSSAALSEETEKPKLKATGHVFQALQYLRKLCNHPALVLTPQHPEFKSTTEKLTVQNSSLHDIQHAPKLSALKQLLLDCGLGNGSSTESGTESVVAQHRILIFCQLKSMLDIVEHDLLKPHLPSVTYLRLDGSIPPGQRHSIVSRFNNDPSIDVLLLTTHVGGLGLNLTGADTVVFVEHDWNPMRDLQAMDRAHRIGQKRVVNVYRLITRGTLEEKIMGLQKFKMNIANTVISQENSSLQSMGTDQLLDLFTLDKDGKAEKADSSTSGKASMKSVLENLSDLWDAEQYDSEYNLETFMRSLE

Target ORF information:

RefSeq Version	NM_001080706.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus B-TFIID TATA-box binding protein associated factor 1 (Btaf1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001080706.1

ORF Insert Sequence:

ATGGCGGTCT CCAGGCTTGA TCGTCTTTTT ATCTTACTGG ATACTGGTAC TACTCCAGTT 
ACAAGAAAGG CTGCTGCACA GCAACTTGGA GAAGTGGTGA AGCTTCATCC TCATGAATTA 
AATAATTTGT TATCTAAGGT ATTAATATAT TTAAGGAGTA CAAATTGGGA TACCCGGATT 
GCAGCAGGAC AAGCTGTTGA AGCTATAGTG AAAAACGTGC CTGAGTGGAA TCCAGTGCCA 
AGAACCAAAC AAGAACCTAC AGAGAGTTCT ATGGAAGATT CATCTACTAC AGATCGATTA 
AATTTTGACA GATTTGATAT ATGCAGATTG TTACAGCATG GGGCATCACT TTTGGGGTCT 
GCTGGTGCAG AATTTGAAGT CCAAGATGAA AAATCAGGTG AAGTGGATCC TAAAGAGAGG 
ATAGCACGCC AACGGAAATT ATTACAGAAG AAACTTGGAC TTAATATGGG AGAAGCAATT 
GGAATGAGTA CTGAAGAACT CTTCAACGAT GAAGATTTGG ATTATACCCC AACTTCAGCA 
GCCCTTGTAA ACAAACAATC TACTCTCCAA GCAGCTGAAT TGATTGATTC AGAATTCCGA 
GCAGGAATGA GTAATAGACA GAAGAACAAA GCTAAAAGAA TGGCCAAGTT ATTTGCAAAA 
CAGAGATCCA GGGATGCAGT GGAAACGAAT GAGAAGAGCA ATGACAGCAC TGATGGAGAG 
CCAGAAGAAA AGAGACGGAA AGTAGCAAAT GTTGTTATTA ATCAGACTGC AAGTGATTCC 
AAAGTCTTGA TAGATAATGT TCCAGAAAGT TCTTCCTTAA TTGAAGAGAC AAATGAATGG 
CCTTTGGAAA GCTTTTGTGA AGAACTTTGC AATGACCTTT TTAATCCCTC CTGGGAGGTT 
CGACATGGGG CAGGTACTGG ACTCAGAGAA ATTCTTAAAG CTCATGGGAA AAGTGGTGGT 
AAAATGGGAG ACAGCACTTT AGAGGAGATG ATTCAGCAGC ATCAAGAGTG GTTGGAAGAC 
TTAGTCATTA GACTCCTTTG TGTTTTTGCG TTGGACAGAT TTGGAGACTT CGTTTCTGAT 
GAAGTTGTAG CGCCAGTTCG TGAGACTTGT GCTCAAACGT TAGGTGTGGT TTTAAAGCAC 
ATGAACGAAA CAGGAGTTCA TAAGACAGTG GATGTGCTGC TGAAATTATT GACACAAGAA 
CAGTGGGAAG TTAGACATGG TGGTCTTCTG GGAATAAAAT ATGCTTTGGC AGTTCGTCAG 
GATGTAATTA ATACTTTATT GCCTAAAGTC TTAACTCGAA TAATCGAAGG ACTCCAGGAC 
CTTGATGACG ATGTTAGAGC TGTTGCTGCA GCATCCCTGG TTCCTGTAGT AGAAAGCCTT 
GTCTACCTTC AGACACAAAA GGTACCCTCC ATTATAAATA CCTTATGGGA TTCTCTTCTG 
GAATTAGATG ATCTAACAGC TTCAACAAAT AGTATTATGA CTCTCCTTTC ATCCATGTTA 
ACTTACCCTC AGGTCCAGCA ATGCAGTATT CAACAGTCAC TCACAGTTTT AGTCCCACGT 
GTCTGGCCAT TTTTGCATCA CACTATATCT TCAGTTCGAA GAGCAGCATT GGAAACTCTC 
TTTACATTAT TATCAACACA GGACGAGAAC TCTTCATCTT GGCTCATACC TATCCTGTCG 
GATATGCTGA GACACATTTT TCAGTTTTGT GTTTTAGAAA GCAGCCAGGA AATTCTGGAT 
CTTATTCACA AGGTTTGGAT GGAGCTTTTG AGCAAGGCGT CAGTTCAGTA TTTGGTAGCA 
GCTGCATGCC CATGGATGAG TGCTTGGCTT TGCCTAATGA TGCAGCCTTC TCACTTACCT 
ATTGATTTAA ATATGCTGCT GGAAGTAAAA GCTAGAGCCA AGGAAAAAAC AGGTGGTAAG 
GTACGGCAAG GTCAAATCCA GAATAAAGAA GTGCTTCAGG AGTATATTGC TGGTGCAGAC 
ACCATCATGG AAGACCCAGC GACCAGAGAT TTTGTAGTTA TGAGGGCCAG GATGATGGCA 
GCTAAGTTGT TAGGAGCACT CTGTTGCTAT ATTTGTGATC CAAGTGTAAA TATGGTAAAC 
CAGGAAATTA AACCAGCGGA TTCCCTTGGC CAGTTACTGC TCTTCTATTT GAATTCCAAA 
TCTGCTTTAC AGAGAATTAG TGTTGCGTTA GTAATCTGTG AGTGGGCTGC TTTACAAAAG 
GAGTGTAAAG CTGTTACCCT AGCTGTGCAA CCGCGTTTAC TTGATATCCT TTCAGAACAT 
TTGTATTATG ATGAAATTGC TGTTCCGTTC ACACGTATGC AGAATGAATG TAAACAGTTT 
ATATCATCAT TAGCTGATGC ACATATTGAA GTTGGTAATA GAGTAAACAA CAATGTTTTA 
ACAATAGATC AAGCAAACGA CCTGGTAACC ACTATTTTTA ATGAAGTCAC ATCAACTTTT 
GATTTAAATC CTCAAGTGTT ACAGCAGTTA GATAGTAAAC GGCATCAGGT CCAAATGACA 
GTTGCAGAGA CGAACCAGGA GTGGCAAGTA CTGCAGCTGA GAGTACACAC GTTTGCTGCG 
TGTGCTGTTG TGAGCTTGCA GCAGCTTCCG GAGAAACTAA ATCCTATCAT AAAACCATTA 
ATGGAAACGA TTAAAAAAGA AGAGAATACG CTAGTGCAGA ACTATGCTGC TCAGTACATA 
GCTAAGCTTC TTCAGCAGTG TACAACAAGG ACACCATGCC CTAATTCAAA AGTTATTAAG 
AACCTCTGTA GCTCTCTTTG TGTGGACCCA TACCTCACTC CCTGTGTTAC GTGTCCAGTA 
CCAACACAAA GTGGCCAGGA AAATTCTAAA GGATCCAACT CAGAAAAGGA TGGAATGCAC 
CATACTGTCA CCAAGCACAG GGGGATCATT ACACTCTACA GGCATCAGAA AGCAGCATTT 
GCTATCACCA GCAGGCGAGG TCCCATCCCC AAAGCAATCA AAGCTCAGAT AGCAGATCTA 
CCTGCAGGAA GTAGTGGGAC TATCCTGGTT GAACTTGATG AGGGTCAGAA GCCTTACCTG 
GTACAGCGGA GAGGAGCTGA ATTTGCCTTA ACAACTATAG TGAAGCATTT TGGTGCTGAA 
ATGGCAGTGA AGTTGCCACA TCTTTGGGAT GCTATGGTTG GCCCATTGAA GTCTATGATT 
GACCTAAATA ATTTTGATGG AAAGTCCCTC CTGGAAAGAG GTGATGTTCC TGCTCAAGAA 
TTGGTGAATT CCCTGCAAGT GTTTGAAATA GCAGCAGCTT CCATGGACTC TGCACTTCAT 
CCCTTGCTGG TACAGCACTT GCCTCATCTT TATATGTGCC TTCAGTACCC CAGCACTGCA 
GTGAGGCACA TGGCGGCTCG TTGCATAGGT GTCATGAGTA AAATAGCTAC CATGGAAACA 
ATGAATATTT TTTTGGAGAA GGTACTTCCT TGGTTGGGAG CAATTGATGA CAATGTCAAG 
CAAGAAGGTG CAATTGAAGC ACTTGCCTGT GTTATGGAAC AATTGGATGT CGGTATTGTT 
CCATATATTG TCCTTTTAGT TGTTCCTGTA TTGGGAAGGA TGAGTGATCA GACAGACAGT 
GTGAGATTCA TGGCTACTCA GTGCTTTGCA ACACTGATTA GACTGATGCC ACTTGAGGCA 
GGCATTCCAG ACCCTCCAAA CATGTCAGAA GAATTAATCC AGTTGAAAGC AAAAGAGCGA 
CACTTTTTAG AGCAATTGTT AGATGGGAAG AAGTTAGAAA ATTACAAGAT CCCAGTGCCA 
ATCAATGCTG AACTCAGGAA ATATCAGCAG GATGGTGTGA ACTGGTTAGC CTTTCTCAAT 
AAGTATAAGC TTCATGGAAT TCTGTGTGAT GACATGGGCT TAGGAAAAAC ATTACAGTCC 
ATCTGCATTC TAGCAGGAGA TCACTGTCAG AGGGCCCAGG AATATGCAAG ATCGAAATTG 
GCAGAATGTA TGCCACTTCC TTCCTTGGTG GTTTGTCCAC CAACATTAAC AGGTCATTGG 
GTAGATGAAG TAGGTAAATT TTGTTCCAGA GAATATCTCA ATCCGTTGCA TTATACTGGC 
CCTCCCACTG AAAGGATAAG GTTGCAACAT CAAGTAAAAA GGCACAATCT GATAGTAGCC 
TCATATGATG TTGTGAGGAA TGACATAGAC TTTTTTAGAA ATATTAAATT TAACTACTGT 
ATTCTTGATG AAGGCCATGT CATAAAAAAT GGAAAAACCA AGTTGTCCAA AGCAGTAAAG 
CAGCTGACTG CTAATTACAG GATTATTCTT TCTGGAACAC CAATCCAGAA CAATGTTTTG 
GAGTTGTGGT CATTATTTGA TTTCCTTATG CCTGGATTTT TGGGTACTGA ACGCCAGTTT 
GCTGCTCGAT ATGGCAAACC TATATTAGCG AGTAGGGATG CACGAAGCTC CAGTCGAGAA 
CAAGAAGCAG GTGTCCTGGC AATGGACGCT CTGCACCGCC AAGTCCTGCC GTTCCTTCTC 
AGAAGAATGA AAGAAGATGT TTTGCAGGAT CTTCCACCTA AAATTATTCA AGACTATTAC 
TGTACTCTCA GCCCTCTCCA GGTTCAGCTT TATGAAGATT TTGCTAAGTC GCGTGCCAAG 
TGTGATGTTG ATGAGACCGT GTCTTCAGCT GCACTTTCAG AAGAAACTGA AAAACCAAAA 
CTTAAAGCGA CAGGTCATGT ATTCCAGGCG TTACAATATT TACGTAAGCT CTGCAACCAT 
CCAGCACTGG TCTTAACCCC TCAGCATCCA GAATTCAAGA GCACTACTGA AAAACTGACC 
GTTCAGAATT CTTCTCTTCA TGATATTCAA CACGCGCCTA AGCTCTCAGC CTTGAAACAA 
CTGCTGTTGG ACTGTGGTTT GGGGAATGGC AGTTCTACAG AGAGTGGCAC AGAGTCTGTT 
GTGGCACAGC ACAGAATACT GATCTTCTGT CAGCTTAAAA GCATGCTTGA TATAGTAGAA 
CATGATCTGC TCAAGCCTCA CCTGCCCTCT GTCACTTACT TGAGACTAGA TGGCAGCATA 
CCTCCTGGAC AGCGGCATTC CATTGTTTCC CGGTTTAACA ACGACCCCTC CATCGATGTT 
CTTCTACTCA CAACTCACGT TGGTGGCCTG GGCCTGAACT TGACTGGAGC TGACACTGTG 
GTGTTTGTGG AGCATGACTG GAACCCTATG CGAGATCTGC AGGCCATGGA CCGGGCCCAT 
CGTATTGGGC AGAAACGTGT GGTTAATGTC TACCGGTTGA TAACCAGAGG AACACTAGAA 
GAAAAGATAA TGGGCTTGCA GAAATTCAAG ATGAACATCG CAAATACTGT TATTAGCCAA 
GAAAACTCTA GCTTACAGAG TATGGGCACT GATCAACTTC TTGACCTGTT CACTCTAGAT 
AAGGATGGAA AAGCAGAAAA GGCTGACAGC TCCACTTCTG GGAAAGCAAG CATGAAATCA 
GTTCTTGAGA ACCTGAGTGA CCTCTGGGAC GCCGAGCAGT ATGATTCGGA GTACAACCTG 
GAGACCTTCA TGCGCTCTCT CGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Btaf1 ( XM_006526561.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006526624.1 Mus musculus B-TFIID TATA-box binding protein associated factor 1 (Btaf1), transcript variant X1, mRNA.

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CloneID	OMu55167
Clone ID Related Accession (Same CDS sequence)	XM_006526561.3 , XM_006526561.4
Accession Version	XM_006526561.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4941bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	TATA-binding protein-associated factor 172 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000085.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006526561.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTAATA GACAGAAGAA CAAAGCTAAA AGAATGGCCA AGTTATTTGC AAAACAGAGA 
TCCAGGGATG CAGTGGAAAC GAATGAGAAG AGCAATGACA GCACTGATGG AGAGCCAGAA 
GAAAAGAGAC GGAAAGTAGC AAATGTTGTT ATTAATCAGA CTGCAAGTGA TTCCAAAGTC 
TTGATAGATA ATGTTCCAGA AAGTTCTTCC TTAATTGAAG AGACAAATGA ATGGCCTTTG 
GAAAGCTTTT GTGAAGAACT TTGCAATGAC CTTTTTAATC CCTCCTGGGA GGTTCGACAT 
GGGGCAGGTA CTGGACTCAG AGAAATTCTT AAAGCTCATG GGAAAAGTGG TGGTAAAATG 
GGAGACAGCA CTTTAGAGGA GATGATTCAG CAGCATCAAG AGTGGTTGGA AGACTTAGTC 
ATTAGACTCC TTTGTGTTTT TGCGTTGGAC AGATTTGGAG ACTTCGTTTC TGATGAAGTT 
GTAGCGCCAG TTCGTGAGAC TTGTGCTCAA ACGTTAGGTG TGGTTTTAAA GCACATGAAC 
GAAACAGGAG TTCATAAGAC AGTGGATGTG CTGCTGAAAT TATTGACACA AGAACAGTGG 
GAAGTTAGAC ATGGTGGTCT TCTGGGAATA AAATATGCTT TGGCAGTTCG TCAGGATGTA 
ATTAATACTT TATTGCCTAA AGTCTTAACT CGAATAATCG AAGGACTCCA GGACCTTGAT 
GACGATGTTA GAGCTGTTGC TGCAGCATCC CTGGTTCCTG TAGTAGAAAG CCTTGTCTAC 
CTTCAGACAC AAAAGGTACC CTCCATTATA AATACCTTAT GGGATTCTCT TCTGGAATTA 
GATGATCTAA CAGCTTCAAC AAATAGTATT ATGACTCTCC TTTCATCCAT GTTAACTTAC 
CCTCAGGTCC AGCAATGCAG TATTCAACAG TCACTCACAG TTTTAGTCCC ACGTGTCTGG 
CCATTTTTGC ATCACACTAT ATCTTCAGTT CGAAGAGCAG CATTGGAAAC TCTCTTTACA 
TTATTATCAA CACAGGACGA GAACTCTTCA TCTTGGCTCA TACCTATCCT GTCGGATATG 
CTGAGACACA TTTTTCAGTT TTGTGTTTTA GAAAGCAGCC AGGAAATTCT GGATCTTATT 
CACAAGGTTT GGATGGAGCT TTTGAGCAAG GCGTCAGTTC AGTATTTGGT AGCAGCTGCA 
TGCCCATGGA TGAGTGCTTG GCTTTGCCTA ATGATGCAGC CTTCTCACTT ACCTATTGAT 
TTAAATATGC TGCTGGAAGT AAAAGCTAGA GCCAAGGAAA AAACAGGTGG TAAGGTACGG 
CAAGGTCAAA TCCAGAATAA AGAAGTGCTT CAGGAGTATA TTGCTGGTGC AGACACCATC 
ATGGAAGACC CAGCGACCAG AGATTTTGTA GTTATGAGGG CCAGGATGAT GGCAGCTAAG 
TTGTTAGGAG CACTCTGTTG CTATATTTGT GATCCAAGTG TAAATATGGT AAACCAGGAA 
ATTAAACCAG CGGATTCCCT TGGCCAGTTA CTGCTCTTCT ATTTGAATTC CAAATCTGCT 
TTACAGAGAA TTAGTGTTGC GTTAGTAATC TGTGAGTGGG CTGCTTTACA AAAGGAGTGT 
AAAGCTGTTA CCCTAGCTGT GCAACCGCGT TTACTTGATA TCCTTTCAGA ACATTTGTAT 
TATGATGAAA TTGCTGTTCC GTTCACACGT ATGCAGAATG AATGTAAACA GTTTATATCA 
TCATTAGCTG ATGCACATAT TGAAGTTGGT AATAGAGTAA ACAACAATGT TTTAACAATA 
GATCAAGCAA ACGACCTGGT AACCACTATT TTTAATGAAG TCACATCAAC TTTTGATTTA 
AATCCTCAAG TGTTACAGCA GTTAGATAGT AAACGGCATC AGGTCCAAAT GACAGTTGCA 
GAGACGAACC AGGAGTGGCA AGTACTGCAG CTGAGAGTAC ACACGTTTGC TGCGTGTGCT 
GTTGTGAGCT TGCAGCAGCT TCCGGAGAAA CTAAATCCTA TCATAAAACC ATTAATGGAA 
ACGATTAAAA AAGAAGAGAA TACGCTAGTG CAGAACTATG CTGCTCAGTA CATAGCTAAG 
CTTCTTCAGC AGTGTACAAC AAGGACACCA TGCCCTAATT CAAAAGTTAT TAAGAACCTC 
TGTAGCTCTC TTTGTGTGGA CCCATACCTC ACTCCCTGTG TTACGTGTCC AGTACCAACA 
CAAAGTGGCC AGGAAAATTC TAAAGGATCC AACTCAGAAA AGGATGGAAT GCACCATACT 
GTCACCAAGC ACAGGGGGAT CATTACACTC TACAGGCATC AGAAAGCAGC ATTTGCTATC 
ACCAGCAGGC GAGGTCCCAT CCCCAAAGCA ATCAAAGCTC AGATAGCAGA TCTACCTGCA 
GGAAGTAGTG GGACTATCCT GGTTGAACTT GATGAGGGTC AGAAGCCTTA CCTGGTACAG 
CGGAGAGGAG CTGAATTTGC CTTAACAACT ATAGTGAAGC ATTTTGGTGC TGAAATGGCA 
GTGAAGTTGC CACATCTTTG GGATGCTATG GTTGGCCCAT TGAAGTCTAT GATTGACCTA 
AATAATTTTG ATGGAAAGTC CCTCCTGGAA AGAGGTGATG TTCCTGCTCA AGAATTGGTG 
AATTCCCTGC AAGTGTTTGA AATAGCAGCA GCTTCCATGG ACTCTGCACT TCATCCCTTG 
CTGGTACAGC ACTTGCCTCA TCTTTATATG TGCCTTCAGT ACCCCAGCAC TGCAGTGAGG 
CACATGGCGG CTCGTTGCAT AGGTGTCATG AGTAAAATAG CTACCATGGA AACAATGAAT 
ATTTTTTTGG AGAAGGTACT TCCTTGGTTG GGAGCAATTG ATGACAATGT CAAGCAAGAA 
GGTGCAATTG AAGCACTTGC CTGTGTTATG GAACAATTGG ATGTCGGTAT TGTTCCATAT 
ATTGTCCTTT TAGTTGTTCC TGTATTGGGA AGGATGAGTG ATCAGACAGA CAGTGTGAGA 
TTCATGGCTA CTCAGTGCTT TGCAACACTG ATTAGACTGA TGCCACTTGA GGCAGGCATT 
CCAGACCCTC CAAACATGTC AGAAGAATTA ATCCAGTTGA AAGCAAAAGA GCGACACTTT 
TTAGAGCAAT TGTTAGATGG GAAGAAGTTA GAAAATTACA AGATCCCAGT GCCAATCAAT 
GCTGAACTCA GGAAATATCA GCAGGATGGT GTGAACTGGT TAGCCTTTCT CAATAAGTAT 
AAGCTTCATG GAATTCTGTG TGATGACATG GGCTTAGGAA AAACATTACA GTCCATCTGC 
ATTCTAGCAG GAGATCACTG TCAGAGGGCC CAGGAATATG CAAGATCGAA ATTGGCAGAA 
TGTATGCCAC TTCCTTCCTT GGTGGTTTGT CCACCAACAT TAACAGGTCA TTGGGTAGAT 
GAAGTAGGTA AATTTTGTTC CAGAGAATAT CTCAATCCGT TGCATTATAC TGGCCCTCCC 
ACTGAAAGGA TAAGGTTGCA ACATCAAGTA AAAAGGCACA ATCTGATAGT AGCCTCATAT 
GATGTTGTGA GGAATGACAT AGACTTTTTT AGAAATATTA AATTTAACTA CTGTATTCTT 
GATGAAGGCC ATGTCATAAA AAATGGAAAA ACCAAGTTGT CCAAAGCAGT AAAGCAGCTG 
ACTGCTAATT ACAGGATTAT TCTTTCTGGA ACACCAATCC AGAACAATGT TTTGGAGTTG 
TGGTCATTAT TTGATTTCCT TATGCCTGGA TTTTTGGGTA CTGAACGCCA GTTTGCTGCT 
CGATATGGCA AACCTATATT AGCGAGTAGG GATGCACGAA GCTCCAGTCG AGAACAAGAA 
GCAGGTGTCC TGGCAATGGA CGCTCTGCAC CGCCAAGTCC TGCCGTTCCT TCTCAGAAGA 
ATGAAAGAAG ATGTTTTGCA GGATCTTCCA CCTAAAATTA TTCAAGACTA TTACTGTACT 
CTCAGCCCTC TCCAGGTTCA GCTTTATGAA GATTTTGCTA AGTCGCGTGC CAAGTGTGAT 
GTTGATGAGA CCGTGTCTTC AGCTGCACTT TCAGAAGAAA CTGAAAAACC AAAACTTAAA 
GCGACAGGTC ATGTATTCCA GGCGTTACAA TATTTACGTA AGCTCTGCAA CCATCCAGCA 
CTGGTCTTAA CCCCTCAGCA TCCAGAATTC AAGAGCACTA CTGAAAAACT GACCGTTCAG 
AATTCTTCTC TTCATGATAT TCAACACGCG CCTAAGCTCT CAGCCTTGAA ACAACTGCTG 
TTGGACTGTG GTTTGGGGAA TGGCAGTTCT ACAGAGAGTG GCACAGAGTC TGTTGTGGCA 
CAGCACAGAA TACTGATCTT CTGTCAGCTT AAAAGCATGC TTGATATAGT AGAACATGAT 
CTGCTCAAGC CTCACCTGCC CTCTGTCACT TACTTGAGAC TAGATGGCAG CATACCTCCT 
GGACAGCGGC ATTCCATTGT TTCCCGGTTT AACAACGACC CCTCCATCGA TGTTCTTCTA 
CTCACAACTC ACGTTGGTGG CCTGGGCCTG AACTTGACTG GAGCTGACAC TGTGGTGTTT 
GTGGAGCATG ACTGGAACCC TATGCGAGAT CTGCAGGCCA TGGACCGGGC CCATCGTATT 
GGGCAGAAAC GTGTGGTTAA TGTCTACCGG TTGATAACCA GAGGAACACT AGAAGAAAAG 
ATAATGGGCT TGCAGAAATT CAAGATGAAC ATCGCAAATA CTGTTATTAG CCAAGAAAAC 
TCTAGCTTAC AGAGTATGGG CACTGATCAA CTTCTTGACC TGTTCACTCT AGATAAGGAT 
GGAAAAGCAG AAAAGGCTGA CAGCTCCACT TCTGGGAAAG CAAGCATGAA ATCAGTTCTT 
GAGAACCTGA GTGACCTCTG GGACGCCGAG CAGTATGATT CGGAGTACAA CCTGGAGACC 
TTCATGCGCT CTCTCGAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006526624.1
CDS	122..5062
Translation	MSNRQKNKAKRMAKLFAKQRSRDAVETNEKSNDSTDGEPEEKRRKVANVVINQTASDSKVLIDNVPESSSLIEETNEWPLESFCEELCNDLFNPSWEVRHGAGTGLREILKAHGKSGGKMGDSTLEEMIQQHQEWLEDLVIRLLCVFALDRFGDFVSDEVVAPVRETCAQTLGVVLKHMNETGVHKTVDVLLKLLTQEQWEVRHGGLLGIKYALAVRQDVINTLLPKVLTRIIEGLQDLDDDVRAVAAASLVPVVESLVYLQTQKVPSIINTLWDSLLELDDLTASTNSIMTLLSSMLTYPQVQQCSIQQSLTVLVPRVWPFLHHTISSVRRAALETLFTLLSTQDENSSSWLIPILSDMLRHIFQFCVLESSQEILDLIHKVWMELLSKASVQYLVAAACPWMSAWLCLMMQPSHLPIDLNMLLEVKARAKEKTGGKVRQGQIQNKEVLQEYIAGADTIMEDPATRDFVVMRARMMAAKLLGALCCYICDPSVNMVNQEIKPADSLGQLLLFYLNSKSALQRISVALVICEWAALQKECKAVTLAVQPRLLDILSEHLYYDEIAVPFTRMQNECKQFISSLADAHIEVGNRVNNNVLTIDQANDLVTTIFNEVTSTFDLNPQVLQQLDSKRHQVQMTVAETNQEWQVLQLRVHTFAACAVVSLQQLPEKLNPIIKPLMETIKKEENTLVQNYAAQYIAKLLQQCTTRTPCPNSKVIKNLCSSLCVDPYLTPCVTCPVPTQSGQENSKGSNSEKDGMHHTVTKHRGIITLYRHQKAAFAITSRRGPIPKAIKAQIADLPAGSSGTILVELDEGQKPYLVQRRGAEFALTTIVKHFGAEMAVKLPHLWDAMVGPLKSMIDLNNFDGKSLLERGDVPAQELVNSLQVFEIAAASMDSALHPLLVQHLPHLYMCLQYPSTAVRHMAARCIGVMSKIATMETMNIFLEKVLPWLGAIDDNVKQEGAIEALACVMEQLDVGIVPYIVLLVVPVLGRMSDQTDSVRFMATQCFATLIRLMPLEAGIPDPPNMSEELIQLKAKERHFLEQLLDGKKLENYKIPVPINAELRKYQQDGVNWLAFLNKYKLHGILCDDMGLGKTLQSICILAGDHCQRAQEYARSKLAECMPLPSLVVCPPTLTGHWVDEVGKFCSREYLNPLHYTGPPTERIRLQHQVKRHNLIVASYDVVRNDIDFFRNIKFNYCILDEGHVIKNGKTKLSKAVKQLTANYRIILSGTPIQNNVLELWSLFDFLMPGFLGTERQFAARYGKPILASRDARSSSREQEAGVLAMDALHRQVLPFLLRRMKEDVLQDLPPKIIQDYYCTLSPLQVQLYEDFAKSRAKCDVDETVSSAALSEETEKPKLKATGHVFQALQYLRKLCNHPALVLTPQHPEFKSTTEKLTVQNSSLHDIQHAPKLSALKQLLLDCGLGNGSSTESGTESVVAQHRILIFCQLKSMLDIVEHDLLKPHLPSVTYLRLDGSIPPGQRHSIVSRFNNDPSIDVLLLTTHVGGLGLNLTGADTVVFVEHDWNPMRDLQAMDRAHRIGQKRVVNVYRLITRGTLEEKIMGLQKFKMNIANTVISQENSSLQSMGTDQLLDLFTLDKDGKAEKADSSTSGKASMKSVLENLSDLWDAEQYDSEYNLETFMRSLE

Target ORF information:

RefSeq Version	XM_006526561.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus B-TFIID TATA-box binding protein associated factor 1 (Btaf1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006526561.3

ORF Insert Sequence:

ATGAGTAATA GACAGAAGAA CAAAGCTAAA AGAATGGCCA AGTTATTTGC AAAACAGAGA 
TCCAGGGATG CAGTGGAAAC GAATGAGAAG AGCAATGACA GCACTGATGG AGAGCCAGAA 
GAAAAGAGAC GGAAAGTAGC AAATGTTGTT ATTAATCAGA CTGCAAGTGA TTCCAAAGTC 
TTGATAGATA ATGTTCCAGA AAGTTCTTCC TTAATTGAAG AGACAAATGA ATGGCCTTTG 
GAAAGCTTTT GTGAAGAACT TTGCAATGAC CTTTTTAATC CCTCCTGGGA GGTTCGACAT 
GGGGCAGGTA CTGGACTCAG AGAAATTCTT AAAGCTCATG GGAAAAGTGG TGGTAAAATG 
GGAGACAGCA CTTTAGAGGA GATGATTCAG CAGCATCAAG AGTGGTTGGA AGACTTAGTC 
ATTAGACTCC TTTGTGTTTT TGCGTTGGAC AGATTTGGAG ACTTCGTTTC TGATGAAGTT 
GTAGCGCCAG TTCGTGAGAC TTGTGCTCAA ACGTTAGGTG TGGTTTTAAA GCACATGAAC 
GAAACAGGAG TTCATAAGAC AGTGGATGTG CTGCTGAAAT TATTGACACA AGAACAGTGG 
GAAGTTAGAC ATGGTGGTCT TCTGGGAATA AAATATGCTT TGGCAGTTCG TCAGGATGTA 
ATTAATACTT TATTGCCTAA AGTCTTAACT CGAATAATCG AAGGACTCCA GGACCTTGAT 
GACGATGTTA GAGCTGTTGC TGCAGCATCC CTGGTTCCTG TAGTAGAAAG CCTTGTCTAC 
CTTCAGACAC AAAAGGTACC CTCCATTATA AATACCTTAT GGGATTCTCT TCTGGAATTA 
GATGATCTAA CAGCTTCAAC AAATAGTATT ATGACTCTCC TTTCATCCAT GTTAACTTAC 
CCTCAGGTCC AGCAATGCAG TATTCAACAG TCACTCACAG TTTTAGTCCC ACGTGTCTGG 
CCATTTTTGC ATCACACTAT ATCTTCAGTT CGAAGAGCAG CATTGGAAAC TCTCTTTACA 
TTATTATCAA CACAGGACGA GAACTCTTCA TCTTGGCTCA TACCTATCCT GTCGGATATG 
CTGAGACACA TTTTTCAGTT TTGTGTTTTA GAAAGCAGCC AGGAAATTCT GGATCTTATT 
CACAAGGTTT GGATGGAGCT TTTGAGCAAG GCGTCAGTTC AGTATTTGGT AGCAGCTGCA 
TGCCCATGGA TGAGTGCTTG GCTTTGCCTA ATGATGCAGC CTTCTCACTT ACCTATTGAT 
TTAAATATGC TGCTGGAAGT AAAAGCTAGA GCCAAGGAAA AAACAGGTGG TAAGGTACGG 
CAAGGTCAAA TCCAGAATAA AGAAGTGCTT CAGGAGTATA TTGCTGGTGC AGACACCATC 
ATGGAAGACC CAGCGACCAG AGATTTTGTA GTTATGAGGG CCAGGATGAT GGCAGCTAAG 
TTGTTAGGAG CACTCTGTTG CTATATTTGT GATCCAAGTG TAAATATGGT AAACCAGGAA 
ATTAAACCAG CGGATTCCCT TGGCCAGTTA CTGCTCTTCT ATTTGAATTC CAAATCTGCT 
TTACAGAGAA TTAGTGTTGC GTTAGTAATC TGTGAGTGGG CTGCTTTACA AAAGGAGTGT 
AAAGCTGTTA CCCTAGCTGT GCAACCGCGT TTACTTGATA TCCTTTCAGA ACATTTGTAT 
TATGATGAAA TTGCTGTTCC GTTCACACGT ATGCAGAATG AATGTAAACA GTTTATATCA 
TCATTAGCTG ATGCACATAT TGAAGTTGGT AATAGAGTAA ACAACAATGT TTTAACAATA 
GATCAAGCAA ACGACCTGGT AACCACTATT TTTAATGAAG TCACATCAAC TTTTGATTTA 
AATCCTCAAG TGTTACAGCA GTTAGATAGT AAACGGCATC AGGTCCAAAT GACAGTTGCA 
GAGACGAACC AGGAGTGGCA AGTACTGCAG CTGAGAGTAC ACACGTTTGC TGCGTGTGCT 
GTTGTGAGCT TGCAGCAGCT TCCGGAGAAA CTAAATCCTA TCATAAAACC ATTAATGGAA 
ACGATTAAAA AAGAAGAGAA TACGCTAGTG CAGAACTATG CTGCTCAGTA CATAGCTAAG 
CTTCTTCAGC AGTGTACAAC AAGGACACCA TGCCCTAATT CAAAAGTTAT TAAGAACCTC 
TGTAGCTCTC TTTGTGTGGA CCCATACCTC ACTCCCTGTG TTACGTGTCC AGTACCAACA 
CAAAGTGGCC AGGAAAATTC TAAAGGATCC AACTCAGAAA AGGATGGAAT GCACCATACT 
GTCACCAAGC ACAGGGGGAT CATTACACTC TACAGGCATC AGAAAGCAGC ATTTGCTATC 
ACCAGCAGGC GAGGTCCCAT CCCCAAAGCA ATCAAAGCTC AGATAGCAGA TCTACCTGCA 
GGAAGTAGTG GGACTATCCT GGTTGAACTT GATGAGGGTC AGAAGCCTTA CCTGGTACAG 
CGGAGAGGAG CTGAATTTGC CTTAACAACT ATAGTGAAGC ATTTTGGTGC TGAAATGGCA 
GTGAAGTTGC CACATCTTTG GGATGCTATG GTTGGCCCAT TGAAGTCTAT GATTGACCTA 
AATAATTTTG ATGGAAAGTC CCTCCTGGAA AGAGGTGATG TTCCTGCTCA AGAATTGGTG 
AATTCCCTGC AAGTGTTTGA AATAGCAGCA GCTTCCATGG ACTCTGCACT TCATCCCTTG 
CTGGTACAGC ACTTGCCTCA TCTTTATATG TGCCTTCAGT ACCCCAGCAC TGCAGTGAGG 
CACATGGCGG CTCGTTGCAT AGGTGTCATG AGTAAAATAG CTACCATGGA AACAATGAAT 
ATTTTTTTGG AGAAGGTACT TCCTTGGTTG GGAGCAATTG ATGACAATGT CAAGCAAGAA 
GGTGCAATTG AAGCACTTGC CTGTGTTATG GAACAATTGG ATGTCGGTAT TGTTCCATAT 
ATTGTCCTTT TAGTTGTTCC TGTATTGGGA AGGATGAGTG ATCAGACAGA CAGTGTGAGA 
TTCATGGCTA CTCAGTGCTT TGCAACACTG ATTAGACTGA TGCCACTTGA GGCAGGCATT 
CCAGACCCTC CAAACATGTC AGAAGAATTA ATCCAGTTGA AAGCAAAAGA GCGACACTTT 
TTAGAGCAAT TGTTAGATGG GAAGAAGTTA GAAAATTACA AGATCCCAGT GCCAATCAAT 
GCTGAACTCA GGAAATATCA GCAGGATGGT GTGAACTGGT TAGCCTTTCT CAATAAGTAT 
AAGCTTCATG GAATTCTGTG TGATGACATG GGCTTAGGAA AAACATTACA GTCCATCTGC 
ATTCTAGCAG GAGATCACTG TCAGAGGGCC CAGGAATATG CAAGATCGAA ATTGGCAGAA 
TGTATGCCAC TTCCTTCCTT GGTGGTTTGT CCACCAACAT TAACAGGTCA TTGGGTAGAT 
GAAGTAGGTA AATTTTGTTC CAGAGAATAT CTCAATCCGT TGCATTATAC TGGCCCTCCC 
ACTGAAAGGA TAAGGTTGCA ACATCAAGTA AAAAGGCACA ATCTGATAGT AGCCTCATAT 
GATGTTGTGA GGAATGACAT AGACTTTTTT AGAAATATTA AATTTAACTA CTGTATTCTT 
GATGAAGGCC ATGTCATAAA AAATGGAAAA ACCAAGTTGT CCAAAGCAGT AAAGCAGCTG 
ACTGCTAATT ACAGGATTAT TCTTTCTGGA ACACCAATCC AGAACAATGT TTTGGAGTTG 
TGGTCATTAT TTGATTTCCT TATGCCTGGA TTTTTGGGTA CTGAACGCCA GTTTGCTGCT 
CGATATGGCA AACCTATATT AGCGAGTAGG GATGCACGAA GCTCCAGTCG AGAACAAGAA 
GCAGGTGTCC TGGCAATGGA CGCTCTGCAC CGCCAAGTCC TGCCGTTCCT TCTCAGAAGA 
ATGAAAGAAG ATGTTTTGCA GGATCTTCCA CCTAAAATTA TTCAAGACTA TTACTGTACT 
CTCAGCCCTC TCCAGGTTCA GCTTTATGAA GATTTTGCTA AGTCGCGTGC CAAGTGTGAT 
GTTGATGAGA CCGTGTCTTC AGCTGCACTT TCAGAAGAAA CTGAAAAACC AAAACTTAAA 
GCGACAGGTC ATGTATTCCA GGCGTTACAA TATTTACGTA AGCTCTGCAA CCATCCAGCA 
CTGGTCTTAA CCCCTCAGCA TCCAGAATTC AAGAGCACTA CTGAAAAACT GACCGTTCAG 
AATTCTTCTC TTCATGATAT TCAACACGCG CCTAAGCTCT CAGCCTTGAA ACAACTGCTG 
TTGGACTGTG GTTTGGGGAA TGGCAGTTCT ACAGAGAGTG GCACAGAGTC TGTTGTGGCA 
CAGCACAGAA TACTGATCTT CTGTCAGCTT AAAAGCATGC TTGATATAGT AGAACATGAT 
CTGCTCAAGC CTCACCTGCC CTCTGTCACT TACTTGAGAC TAGATGGCAG CATACCTCCT 
GGACAGCGGC ATTCCATTGT TTCCCGGTTT AACAACGACC CCTCCATCGA TGTTCTTCTA 
CTCACAACTC ACGTTGGTGG CCTGGGCCTG AACTTGACTG GAGCTGACAC TGTGGTGTTT 
GTGGAGCATG ACTGGAACCC TATGCGAGAT CTGCAGGCCA TGGACCGGGC CCATCGTATT 
GGGCAGAAAC GTGTGGTTAA TGTCTACCGG TTGATAACCA GAGGAACACT AGAAGAAAAG 
ATAATGGGCT TGCAGAAATT CAAGATGAAC ATCGCAAATA CTGTTATTAG CCAAGAAAAC 
TCTAGCTTAC AGAGTATGGG CACTGATCAA CTTCTTGACC TGTTCACTCT AGATAAGGAT 
GGAAAAGCAG AAAAGGCTGA CAGCTCCACT TCTGGGAAAG CAAGCATGAA ATCAGTTCTT 
GAGAACCTGA GTGACCTCTG GGACGCCGAG CAGTATGATT CGGAGTACAA CCTGGAGACC 
TTCATGCGCT CTCTCGAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Mot1, Ino80C, and NC2 Function Coordinately to Regulate Pervasive Transcription in Yeast and Mammals.
Molecular cell67(4)594-607.e4(2017 Aug)
Xue Y,Pradhan SK,Sun F,Chronis C,Tran N,Su T,Van C,Vashisht A,Wohlschlegel J,Peterson CL,Timmers HTM,Kurdistani SK,Carey MF

An ENU-induced point mutation in the mouse Btaf1 gene causes post-gastrulation embryonic lethality and protein instability.
Mechanisms of development128(5-6)279-88(2011 May-Jun)
Wansleeben C,van Gurp L,de Graaf P,Mousson F,Marc Timmers HT,Meijlink F

BayGenomics: a resource of insertional mutations in mouse embryonic stem cells.
Nucleic acids research31(1)278-81(2003 Jan)
Stryke D,Kawamoto M,Huang CC,Johns SJ,King LA,Harper CA,Meng EC,Lee RE,Yee A,L'Italien L,Chuang PT,Young SG,Skarnes WC,Babbitt PC,Ferrin TE

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Btaf1 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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