Iars cDNA ORF clone, Mus musculus(house mouse)

The following Iars gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Iars cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OMu03174 NM_172015.3
Latest version!
Mus musculus isoleucine-tRNA synthetase (Iars), mRNA. pcDNA3.1-C-(k)DYK or customized vector 16 $643.30
$919.00
OMu03174 XM_006516826.2
Latest version!
Mus musculus isoleucine-tRNA synthetase (Iars), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 16 $643.30
$919.00
View Old Accession Versions >>
OMu03174 XM_006516826.1 Mus musculus isoleucine-tRNA synthetase (Iars), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 16 $643.30
$919.00
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OMu03174
    Clone ID Related Accession (Same CDS sequence) XM_006516826.1 , XM_006516826.2 , NM_172015.3
    Accession Version NM_172015.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3789bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-10-20
    Organism Mus musculus(house mouse)
    Product isoleucine--tRNA ligase, cytoplasmic
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BY139910.1, AK133856.1 and BB052629.1. On Jul 22, 2009 this sequence version replaced NM_172015.2. ##Evidence-Data-START## Transcript exon combination :: BC067029.1, SRR1660811.10450.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGGTTCAAC AAGTTCCAGA AAACATCAGT TTTCCTGCTG AAGAAGAAAA AATCTTGGAG 
    TTCTGGTCCA AACATAACTG TTTCCAGGAA TGCCTCAAAC AGTCAAAACT CAGGCCAAAG
    TTTACCTTCT ATGATGGGCC TCCCTTTGCT ACTGGACTGC CTCACTATGG ACACATACTT
    GCTGGGACAA TCAAAGATAT CGTTACGAGA TACGCTCACC AGAGCGGGTT TCACGTTGAC
    AGAAGATTCG GGTGGGACTG TCACGGCTTA CCTGTGGAGT ACGAAATCGA TAAGACACTG
    GGCATCAAAG GCCCGGAGGA TGTGGCCAAA ATGGGGATTG CAGAGTACAA CAAGCAGTGC
    CGAGCAATCG TGATGAGATA CTCCGCTGAG TGGAAGTCTA CTGTTACCAG ACTCGGCCGA
    TGGATCGACT TTGACAATGA CTACAAAACA CTATACCCAC AGTTCATGGA ATCAGTCTGG
    TGGGTCTTCA AGCAGCTGTA TGACAAAGGC CTCGTGTACA GAGGCGTGAA GGTCATGCCC
    TTCTCCACCG CGTGTGGCAC TCCACTCTCC AACTTCGAAT CTAACCAGAA TTACAAGGAT
    GTTCAGGATC CCTCAGTATT TGTAACATTC CCTTTGGAGG AAGATGAAAA TACATCCTTA
    GTTGCTTGGA CAACCACTCC TTGGACTCTA CCCAGTAACC TTGCACTATG CGTCAATCCA
    GAAATACAGT ATGTGAAGAT CAAAGATGTG GCCAGGGGAA AATTATTCAT CTTAACGGAA
    GCCAGATTAT CGGCCCTCTA TAAGCAAGAG AGCGACTATG AGATACTTGA AAGGTTTCCT
    GGTGCCTCTC TCAAAGGCAA GAAATACAAG CCGTTGTTTG ACTACTTTAT TAAGTGTAAG
    GAGAACGGTG CCTTCACCGT GCTTGTCGAC CACTATGTGA AGGATGAAGA AGGCACAGGC
    GTTGTGCACC AGGCTCCTTA CTTTGGTGCT GATGATCATC GTGTCTGTAT GGACTTCAAC
    ATTATTCAGA AAGACTCAGT CCCTGTTTGT CCTGTGGATG CTTCAGGCTG CTTCACAGAG
    GAAGTGACAC ATTTTGTGGG GCAGTATGTG AAGGATGCTG ACAAAAATAT CATTAGGATG
    TTGAAGGAGC AGGGCCGGCT GCTGGCGGCG GGCACGTTCA CTCACAGCTA CCCTTTCTGC
    TGGAGGTCAG ACACGCCCCT CATTTACAAG TCTGTGCCCA GCTGGTTTGT GCGGGTGGAG
    CCCATGGTGG ACCAGCTACT GAAGAACAAC GACCTGTGCT ACTGGGTCCC AGAGTTTGTA
    CGAGAAAAAC GGTTTGGAAA TTGGCTGAAG GAGGCCCGTG ACTGGGCAAT TTCCAGAAAC
    AGATACTGGG GCACCCCCAT CCCACTGTGG GTCAGCGAAG ACTTGGAGGA GGTAGTGTGC
    ATTGGATCAG TGGCAGAGCT TGAAGAACTG TCGGGAACAA AAATCTCAGA TCTCCACAGA
    GAGAGCATCG ACCACCTGAC CATCCCATCC CGCTGCGGGA AAGCACCATT GCGGCGCGTC
    TCTGAGGTGT TTGACTGCTG GTTCGAGAGT GGAAGTATGC CCTATGCCCA GGTCCATTAT
    CCGTTCGAGA GCAAGCGCGA GTTTGAGGAT GCCTTTCCTG CAGACTTCAT TGCTGAAGGC
    ATCGATCAAA CCAGAGGATG GTTTTACACC CTGCTGGTGC TAGCCACAGC GCTCTTTGGA
    CAGCCACCTT TCAAGAATGT GATTGTGAAT GGCCTCATCT TGGCAAGTGA TGGCCAAAAA
    ATGAGCAAGC GGAAAAAGAA CTACCCAGAT CCAGTTTCCA TCATCGATAA GTATGGTGCG
    GACGCCCTCA GGTTATATCT GATCAATTCC CCTGTGGTAA GAGCAGAAAA CCTGCGCTTC
    AAGGAGGAAG GTGTGCGGGA TGTCCTCAAA GATGTGCTGC TCCCGTGGTA CAACGCTTAC
    CGCTTCTTCA TCCAGAACGT CTTTAGGCTC CACAAGGAAG AAGAAGTGAA GTTCCTATAC
    AACGAGCATA CAGTCAGAGA AAGCCCTAAC ATCACCGACC GCTGGGTTCT GTCCTTCATG
    CAGTCGCTCC TTGGCTTCTT TGAGACCGAA ATGGCAGCTT ACAGGCTGTA CACTGTGGTA
    CCTCGCCTGG TCAAGTTTGT AGATATTCTA ACCAACTGGT ATGTCCGAAT GAACAGGAGG
    AGGTTAAAGG GTGAGAGTGG GGTGGAAGAC TGTGTCATGG CTCTGGAGAC ACTGTTCAGT
    GTCCTGCTTT CTCTGTGCAG ACTGATGGCC CCTTACACGC CCTTTCTGAC TGAGCTGATG
    TACCAGAATC TAAAGCTGCT GATTGATCCT GCTTCTCTCC GGGACAAGGA CACACTCAGC
    ATCCACTACC TCATGCTGCC TCGAGTTCGA GAGGAGCTGA TTGACAAGAA AACTGAGAAC
    GCCGTGTCTA GGATGCAGTC TGTGATCGAG CTTGGAAGAG TGATACGAGA CCGAAAAACT
    ATTCCAATAA AGTATCCTCT GAAGGAAATT GTGGTCATCC ACCAAGACCC AGAAGCTCTA
    GAGGATATCA GGTCCTTGGA GAAGTATATC ATTGAGGAAT TAAATGTTCG AAAAGTCACG
    CTGTCTACTG ATAAAAACAA GTACGGTATC CGACTAAGGG CTGAACCAGA TCACATGGTC
    CTTGGGAAGC GCCTGAAGGG GGCCTTCAAA GCGGTGATGA TGGCCATAAA GCGGCTGAGC
    AATGAGGAGC TGGAGCGGTT TCAGAAGAGC GGGAGCATTG TTGTGGAAGG CCACGAACTG
    CATGAGGAAG ACATTCGCCT CATGTACACC TTTGACCAGG CCACGGGAGG GACCGCGCAG
    TTTGAAGCAC ACTCTGATGC TCAGGCTCTA GTTCTCTTAG ATGTCACACC TGACCAGTCA
    ATGGTGGATG AGGGCATGGC TCGGGAAGTC ATCAATCGCA TCCAGAAGCT TCGAAAGAAG
    TGCAATCTGG TTCCAACGGA TGAAATAACA GTTTACTATA ATGCAAAATC TGAAGGAAGA
    TACTTGAATA ATGTCATTGA AAGCCACACT GACTTCATAT TTGCCACAAT CAAAGCTCCC
    TTGAAGCCAT ATCCAGTTCC AACATCAGAT AATATCCTTA TTCAAGAGCA AACACAGTTA
    AAGGGGTCCG AGCTGGAAAT CACGCTCACC AAAGGATCGT GTGTGCCTGG CCCTGCTTGT
    GCTTATGTGA ACCTTAACAT TTGTGCAAAT GGCACCGAGC AAGGTGGAGT GTTGCTCCTG
    GAGAATCCGA AAGGTGATAA TCAGCTGAAC CTGGTGAAAC TGAAGACTGT TGTTACCAGC
    GTATTTGGTG TTAAAAATGC AAAGCTGTCA GTCTTCCACG GTGAAACAGA AATACAGAAC
    CAAACTGACT TGCTGAGCCT CAGTGGGAGG ACGCTGTGTG TGACTGCAGG GGCATCCCCG
    TCTCCCATCA GCAGCCCCAG CACCCTGCTG TGTCAGTACC TCAACCTGCA GCTCCTGAAC
    GCAGAGCCAC AAGAGTGTTT AACAGGAACT GTGGGCACCC TCCTGCTTGA AAACCCACTC
    GGACAGAACG GCCTCACCCA CCAAGGGCTT GTGCATGAAG CAGCCAAGGT GTTCGGACTG
    AGGAGCAGGA GGCTAAGGCT GTTCCTGAAT GAGACTCAAA CACAAGAAAT TACAGAAGAT
    ATCCCAATGA AGACTTTGAA TATGAAGACT GTATATGTTT CTGTCTTGCC AACAACTGCA
    GATGGCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_742012.2
    CDS82..3870
    Translation

    Target ORF information:

    RefSeq Version NM_172015.3
    Organism Mus musculus(house mouse)
    Definition Mus musculus isoleucine-tRNA synthetase (Iars), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_172015.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGGTTCAAC AAGTTCCAGA AAACATCAGT TTTCCTGCTG AAGAAGAAAA AATCTTGGAG 
    TTCTGGTCCA AACATAACTG TTTCCAGGAA TGCCTCAAAC AGTCAAAACT CAGGCCAAAG
    TTTACCTTCT ATGATGGGCC TCCCTTTGCT ACTGGACTGC CTCACTATGG ACACATACTT
    GCTGGGACAA TCAAAGATAT CGTTACGAGA TACGCTCACC AGAGCGGGTT TCACGTTGAC
    AGAAGATTCG GGTGGGACTG TCACGGCTTA CCTGTGGAGT ACGAAATCGA TAAGACACTG
    GGCATCAAAG GCCCGGAGGA TGTGGCCAAA ATGGGGATTG CAGAGTACAA CAAGCAGTGC
    CGAGCAATCG TGATGAGATA CTCCGCTGAG TGGAAGTCTA CTGTTACCAG ACTCGGCCGA
    TGGATCGACT TTGACAATGA CTACAAAACA CTATACCCAC AGTTCATGGA ATCAGTCTGG
    TGGGTCTTCA AGCAGCTGTA TGACAAAGGC CTCGTGTACA GAGGCGTGAA GGTCATGCCC
    TTCTCCACCG CGTGTGGCAC TCCACTCTCC AACTTCGAAT CTAACCAGAA TTACAAGGAT
    GTTCAGGATC CCTCAGTATT TGTAACATTC CCTTTGGAGG AAGATGAAAA TACATCCTTA
    GTTGCTTGGA CAACCACTCC TTGGACTCTA CCCAGTAACC TTGCACTATG CGTCAATCCA
    GAAATACAGT ATGTGAAGAT CAAAGATGTG GCCAGGGGAA AATTATTCAT CTTAACGGAA
    GCCAGATTAT CGGCCCTCTA TAAGCAAGAG AGCGACTATG AGATACTTGA AAGGTTTCCT
    GGTGCCTCTC TCAAAGGCAA GAAATACAAG CCGTTGTTTG ACTACTTTAT TAAGTGTAAG
    GAGAACGGTG CCTTCACCGT GCTTGTCGAC CACTATGTGA AGGATGAAGA AGGCACAGGC
    GTTGTGCACC AGGCTCCTTA CTTTGGTGCT GATGATCATC GTGTCTGTAT GGACTTCAAC
    ATTATTCAGA AAGACTCAGT CCCTGTTTGT CCTGTGGATG CTTCAGGCTG CTTCACAGAG
    GAAGTGACAC ATTTTGTGGG GCAGTATGTG AAGGATGCTG ACAAAAATAT CATTAGGATG
    TTGAAGGAGC AGGGCCGGCT GCTGGCGGCG GGCACGTTCA CTCACAGCTA CCCTTTCTGC
    TGGAGGTCAG ACACGCCCCT CATTTACAAG TCTGTGCCCA GCTGGTTTGT GCGGGTGGAG
    CCCATGGTGG ACCAGCTACT GAAGAACAAC GACCTGTGCT ACTGGGTCCC AGAGTTTGTA
    CGAGAAAAAC GGTTTGGAAA TTGGCTGAAG GAGGCCCGTG ACTGGGCAAT TTCCAGAAAC
    AGATACTGGG GCACCCCCAT CCCACTGTGG GTCAGCGAAG ACTTGGAGGA GGTAGTGTGC
    ATTGGATCAG TGGCAGAGCT TGAAGAACTG TCGGGAACAA AAATCTCAGA TCTCCACAGA
    GAGAGCATCG ACCACCTGAC CATCCCATCC CGCTGCGGGA AAGCACCATT GCGGCGCGTC
    TCTGAGGTGT TTGACTGCTG GTTCGAGAGT GGAAGTATGC CCTATGCCCA GGTCCATTAT
    CCGTTCGAGA GCAAGCGCGA GTTTGAGGAT GCCTTTCCTG CAGACTTCAT TGCTGAAGGC
    ATCGATCAAA CCAGAGGATG GTTTTACACC CTGCTGGTGC TAGCCACAGC GCTCTTTGGA
    CAGCCACCTT TCAAGAATGT GATTGTGAAT GGCCTCATCT TGGCAAGTGA TGGCCAAAAA
    ATGAGCAAGC GGAAAAAGAA CTACCCAGAT CCAGTTTCCA TCATCGATAA GTATGGTGCG
    GACGCCCTCA GGTTATATCT GATCAATTCC CCTGTGGTAA GAGCAGAAAA CCTGCGCTTC
    AAGGAGGAAG GTGTGCGGGA TGTCCTCAAA GATGTGCTGC TCCCGTGGTA CAACGCTTAC
    CGCTTCTTCA TCCAGAACGT CTTTAGGCTC CACAAGGAAG AAGAAGTGAA GTTCCTATAC
    AACGAGCATA CAGTCAGAGA AAGCCCTAAC ATCACCGACC GCTGGGTTCT GTCCTTCATG
    CAGTCGCTCC TTGGCTTCTT TGAGACCGAA ATGGCAGCTT ACAGGCTGTA CACTGTGGTA
    CCTCGCCTGG TCAAGTTTGT AGATATTCTA ACCAACTGGT ATGTCCGAAT GAACAGGAGG
    AGGTTAAAGG GTGAGAGTGG GGTGGAAGAC TGTGTCATGG CTCTGGAGAC ACTGTTCAGT
    GTCCTGCTTT CTCTGTGCAG ACTGATGGCC CCTTACACGC CCTTTCTGAC TGAGCTGATG
    TACCAGAATC TAAAGCTGCT GATTGATCCT GCTTCTCTCC GGGACAAGGA CACACTCAGC
    ATCCACTACC TCATGCTGCC TCGAGTTCGA GAGGAGCTGA TTGACAAGAA AACTGAGAAC
    GCCGTGTCTA GGATGCAGTC TGTGATCGAG CTTGGAAGAG TGATACGAGA CCGAAAAACT
    ATTCCAATAA AGTATCCTCT GAAGGAAATT GTGGTCATCC ACCAAGACCC AGAAGCTCTA
    GAGGATATCA GGTCCTTGGA GAAGTATATC ATTGAGGAAT TAAATGTTCG AAAAGTCACG
    CTGTCTACTG ATAAAAACAA GTACGGTATC CGACTAAGGG CTGAACCAGA TCACATGGTC
    CTTGGGAAGC GCCTGAAGGG GGCCTTCAAA GCGGTGATGA TGGCCATAAA GCGGCTGAGC
    AATGAGGAGC TGGAGCGGTT TCAGAAGAGC GGGAGCATTG TTGTGGAAGG CCACGAACTG
    CATGAGGAAG ACATTCGCCT CATGTACACC TTTGACCAGG CCACGGGAGG GACCGCGCAG
    TTTGAAGCAC ACTCTGATGC TCAGGCTCTA GTTCTCTTAG ATGTCACACC TGACCAGTCA
    ATGGTGGATG AGGGCATGGC TCGGGAAGTC ATCAATCGCA TCCAGAAGCT TCGAAAGAAG
    TGCAATCTGG TTCCAACGGA TGAAATAACA GTTTACTATA ATGCAAAATC TGAAGGAAGA
    TACTTGAATA ATGTCATTGA AAGCCACACT GACTTCATAT TTGCCACAAT CAAAGCTCCC
    TTGAAGCCAT ATCCAGTTCC AACATCAGAT AATATCCTTA TTCAAGAGCA AACACAGTTA
    AAGGGGTCCG AGCTGGAAAT CACGCTCACC AAAGGATCGT GTGTGCCTGG CCCTGCTTGT
    GCTTATGTGA ACCTTAACAT TTGTGCAAAT GGCACCGAGC AAGGTGGAGT GTTGCTCCTG
    GAGAATCCGA AAGGTGATAA TCAGCTGAAC CTGGTGAAAC TGAAGACTGT TGTTACCAGC
    GTATTTGGTG TTAAAAATGC AAAGCTGTCA GTCTTCCACG GTGAAACAGA AATACAGAAC
    CAAACTGACT TGCTGAGCCT CAGTGGGAGG ACGCTGTGTG TGACTGCAGG GGCATCCCCG
    TCTCCCATCA GCAGCCCCAG CACCCTGCTG TGTCAGTACC TCAACCTGCA GCTCCTGAAC
    GCAGAGCCAC AAGAGTGTTT AACAGGAACT GTGGGCACCC TCCTGCTTGA AAACCCACTC
    GGACAGAACG GCCTCACCCA CCAAGGGCTT GTGCATGAAG CAGCCAAGGT GTTCGGACTG
    AGGAGCAGGA GGCTAAGGCT GTTCCTGAAT GAGACTCAAA CACAAGAAAT TACAGAAGAT
    ATCCCAATGA AGACTTTGAA TATGAAGACT GTATATGTTT CTGTCTTGCC AACAACTGCA
    GATGGCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu03174
    Clone ID Related Accession (Same CDS sequence) XM_006516826.1 , XM_006516826.2 , NM_172015.3
    Accession Version XM_006516826.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3789bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-02-08
    Organism Mus musculus(House mouse)
    Product isoleucine--tRNA ligase, cytoplasmic isoform X1
    Comment

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGGTTCAAC AAGTTCCAGA AAACATCAGT TTTCCTGCTG AAGAAGAAAA AATCTTGGAG 
    TTCTGGTCCA AACATAACTG TTTCCAGGAA TGCCTCAAAC AGTCAAAACT CAGGCCAAAG
    TTTACCTTCT ATGATGGGCC TCCCTTTGCT ACTGGACTGC CTCACTATGG ACACATACTT
    GCTGGGACAA TCAAAGATAT CGTTACGAGA TACGCTCACC AGAGCGGGTT TCACGTTGAC
    AGAAGATTCG GGTGGGACTG TCACGGCTTA CCTGTGGAGT ACGAAATCGA TAAGACACTG
    GGCATCAAAG GCCCGGAGGA TGTGGCCAAA ATGGGGATTG CAGAGTACAA CAAGCAGTGC
    CGAGCAATCG TGATGAGATA CTCCGCTGAG TGGAAGTCTA CTGTTACCAG ACTCGGCCGA
    TGGATCGACT TTGACAATGA CTACAAAACA CTATACCCAC AGTTCATGGA ATCAGTCTGG
    TGGGTCTTCA AGCAGCTGTA TGACAAAGGC CTCGTGTACA GAGGCGTGAA GGTCATGCCC
    TTCTCCACCG CGTGTGGCAC TCCACTCTCC AACTTCGAAT CTAACCAGAA TTACAAGGAT
    GTTCAGGATC CCTCAGTATT TGTAACATTC CCTTTGGAGG AAGATGAAAA TACATCCTTA
    GTTGCTTGGA CAACCACTCC TTGGACTCTA CCCAGTAACC TTGCACTATG CGTCAATCCA
    GAAATACAGT ATGTGAAGAT CAAAGATGTG GCCAGGGGAA AATTATTCAT CTTAACGGAA
    GCCAGATTAT CGGCCCTCTA TAAGCAAGAG AGCGACTATG AGATACTTGA AAGGTTTCCT
    GGTGCCTCTC TCAAAGGCAA GAAATACAAG CCGTTGTTTG ACTACTTTAT TAAGTGTAAG
    GAGAACGGTG CCTTCACCGT GCTTGTCGAC CACTATGTGA AGGATGAAGA AGGCACAGGC
    GTTGTGCACC AGGCTCCTTA CTTTGGTGCT GATGATCATC GTGTCTGTAT GGACTTCAAC
    ATTATTCAGA AAGACTCAGT CCCTGTTTGT CCTGTGGATG CTTCAGGCTG CTTCACAGAG
    GAAGTGACAC ATTTTGTGGG GCAGTATGTG AAGGATGCTG ACAAAAATAT CATTAGGATG
    TTGAAGGAGC AGGGCCGGCT GCTGGCGGCG GGCACGTTCA CTCACAGCTA CCCTTTCTGC
    TGGAGGTCAG ACACGCCCCT CATTTACAAG TCTGTGCCCA GCTGGTTTGT GCGGGTGGAG
    CCCATGGTGG ACCAGCTACT GAAGAACAAC GACCTGTGCT ACTGGGTCCC AGAGTTTGTA
    CGAGAAAAAC GGTTTGGAAA TTGGCTGAAG GAGGCCCGTG ACTGGGCAAT TTCCAGAAAC
    AGATACTGGG GCACCCCCAT CCCACTGTGG GTCAGCGAAG ACTTGGAGGA GGTAGTGTGC
    ATTGGATCAG TGGCAGAGCT TGAAGAACTG TCGGGAACAA AAATCTCAGA TCTCCACAGA
    GAGAGCATCG ACCACCTGAC CATCCCATCC CGCTGCGGGA AAGCACCATT GCGGCGCGTC
    TCTGAGGTGT TTGACTGCTG GTTCGAGAGT GGAAGTATGC CCTATGCCCA GGTCCATTAT
    CCGTTCGAGA GCAAGCGCGA GTTTGAGGAT GCCTTTCCTG CAGACTTCAT TGCTGAAGGC
    ATCGATCAAA CCAGAGGATG GTTTTACACC CTGCTGGTGC TAGCCACAGC GCTCTTTGGA
    CAGCCACCTT TCAAGAATGT GATTGTGAAT GGCCTCATCT TGGCAAGTGA TGGCCAAAAA
    ATGAGCAAGC GGAAAAAGAA CTACCCAGAT CCAGTTTCCA TCATCGATAA GTATGGTGCG
    GACGCCCTCA GGTTATATCT GATCAATTCC CCTGTGGTAA GAGCAGAAAA CCTGCGCTTC
    AAGGAGGAAG GTGTGCGGGA TGTCCTCAAA GATGTGCTGC TCCCGTGGTA CAACGCTTAC
    CGCTTCTTCA TCCAGAACGT CTTTAGGCTC CACAAGGAAG AAGAAGTGAA GTTCCTATAC
    AACGAGCATA CAGTCAGAGA AAGCCCTAAC ATCACCGACC GCTGGGTTCT GTCCTTCATG
    CAGTCGCTCC TTGGCTTCTT TGAGACCGAA ATGGCAGCTT ACAGGCTGTA CACTGTGGTA
    CCTCGCCTGG TCAAGTTTGT AGATATTCTA ACCAACTGGT ATGTCCGAAT GAACAGGAGG
    AGGTTAAAGG GTGAGAGTGG GGTGGAAGAC TGTGTCATGG CTCTGGAGAC ACTGTTCAGT
    GTCCTGCTTT CTCTGTGCAG ACTGATGGCC CCTTACACGC CCTTTCTGAC TGAGCTGATG
    TACCAGAATC TAAAGCTGCT GATTGATCCT GCTTCTCTCC GGGACAAGGA CACACTCAGC
    ATCCACTACC TCATGCTGCC TCGAGTTCGA GAGGAGCTGA TTGACAAGAA AACTGAGAAC
    GCCGTGTCTA GGATGCAGTC TGTGATCGAG CTTGGAAGAG TGATACGAGA CCGAAAAACT
    ATTCCAATAA AGTATCCTCT GAAGGAAATT GTGGTCATCC ACCAAGACCC AGAAGCTCTA
    GAGGATATCA GGTCCTTGGA GAAGTATATC ATTGAGGAAT TAAATGTTCG AAAAGTCACG
    CTGTCTACTG ATAAAAACAA GTACGGTATC CGACTAAGGG CTGAACCAGA TCACATGGTC
    CTTGGGAAGC GCCTGAAGGG GGCCTTCAAA GCGGTGATGA TGGCCATAAA GCGGCTGAGC
    AATGAGGAGC TGGAGCGGTT TCAGAAGAGC GGGAGCATTG TTGTGGAAGG CCACGAACTG
    CATGAGGAAG ACATTCGCCT CATGTACACC TTTGACCAGG CCACGGGAGG GACCGCGCAG
    TTTGAAGCAC ACTCTGATGC TCAGGCTCTA GTTCTCTTAG ATGTCACACC TGACCAGTCA
    ATGGTGGATG AGGGCATGGC TCGGGAAGTC ATCAATCGCA TCCAGAAGCT TCGAAAGAAG
    TGCAATCTGG TTCCAACGGA TGAAATAACA GTTTACTATA ATGCAAAATC TGAAGGAAGA
    TACTTGAATA ATGTCATTGA AAGCCACACT GACTTCATAT TTGCCACAAT CAAAGCTCCC
    TTGAAGCCAT ATCCAGTTCC AACATCAGAT AATATCCTTA TTCAAGAGCA AACACAGTTA
    AAGGGGTCCG AGCTGGAAAT CACGCTCACC AAAGGATCGT GTGTGCCTGG CCCTGCTTGT
    GCTTATGTGA ACCTTAACAT TTGTGCAAAT GGCACCGAGC AAGGTGGAGT GTTGCTCCTG
    GAGAATCCGA AAGGTGATAA TCAGCTGAAC CTGGTGAAAC TGAAGACTGT TGTTACCAGC
    GTATTTGGTG TTAAAAATGC AAAGCTGTCA GTCTTCCACG GTGAAACAGA AATACAGAAC
    CAAACTGACT TGCTGAGCCT CAGTGGGAGG ACGCTGTGTG TGACTGCAGG GGCATCCCCG
    TCTCCCATCA GCAGCCCCAG CACCCTGCTG TGTCAGTACC TCAACCTGCA GCTCCTGAAC
    GCAGAGCCAC AAGAGTGTTT AACAGGAACT GTGGGCACCC TCCTGCTTGA AAACCCACTC
    GGACAGAACG GCCTCACCCA CCAAGGGCTT GTGCATGAAG CAGCCAAGGT GTTCGGACTG
    AGGAGCAGGA GGCTAAGGCT GTTCCTGAAT GAGACTCAAA CACAAGAAAT TACAGAAGAT
    ATCCCAATGA AGACTTTGAA TATGAAGACT GTATATGTTT CTGTCTTGCC AACAACTGCA
    GATGGCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006516889.1
    Translation

    Target ORF information:

    RefSeq Version XM_006516826.1
    Organism Mus musculus(House mouse)
    Definition Mus musculus isoleucine-tRNA synthetase (Iars), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006516826.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGGTTCAAC AAGTTCCAGA AAACATCAGT TTTCCTGCTG AAGAAGAAAA AATCTTGGAG 
    TTCTGGTCCA AACATAACTG TTTCCAGGAA TGCCTCAAAC AGTCAAAACT CAGGCCAAAG
    TTTACCTTCT ATGATGGGCC TCCCTTTGCT ACTGGACTGC CTCACTATGG ACACATACTT
    GCTGGGACAA TCAAAGATAT CGTTACGAGA TACGCTCACC AGAGCGGGTT TCACGTTGAC
    AGAAGATTCG GGTGGGACTG TCACGGCTTA CCTGTGGAGT ACGAAATCGA TAAGACACTG
    GGCATCAAAG GCCCGGAGGA TGTGGCCAAA ATGGGGATTG CAGAGTACAA CAAGCAGTGC
    CGAGCAATCG TGATGAGATA CTCCGCTGAG TGGAAGTCTA CTGTTACCAG ACTCGGCCGA
    TGGATCGACT TTGACAATGA CTACAAAACA CTATACCCAC AGTTCATGGA ATCAGTCTGG
    TGGGTCTTCA AGCAGCTGTA TGACAAAGGC CTCGTGTACA GAGGCGTGAA GGTCATGCCC
    TTCTCCACCG CGTGTGGCAC TCCACTCTCC AACTTCGAAT CTAACCAGAA TTACAAGGAT
    GTTCAGGATC CCTCAGTATT TGTAACATTC CCTTTGGAGG AAGATGAAAA TACATCCTTA
    GTTGCTTGGA CAACCACTCC TTGGACTCTA CCCAGTAACC TTGCACTATG CGTCAATCCA
    GAAATACAGT ATGTGAAGAT CAAAGATGTG GCCAGGGGAA AATTATTCAT CTTAACGGAA
    GCCAGATTAT CGGCCCTCTA TAAGCAAGAG AGCGACTATG AGATACTTGA AAGGTTTCCT
    GGTGCCTCTC TCAAAGGCAA GAAATACAAG CCGTTGTTTG ACTACTTTAT TAAGTGTAAG
    GAGAACGGTG CCTTCACCGT GCTTGTCGAC CACTATGTGA AGGATGAAGA AGGCACAGGC
    GTTGTGCACC AGGCTCCTTA CTTTGGTGCT GATGATCATC GTGTCTGTAT GGACTTCAAC
    ATTATTCAGA AAGACTCAGT CCCTGTTTGT CCTGTGGATG CTTCAGGCTG CTTCACAGAG
    GAAGTGACAC ATTTTGTGGG GCAGTATGTG AAGGATGCTG ACAAAAATAT CATTAGGATG
    TTGAAGGAGC AGGGCCGGCT GCTGGCGGCG GGCACGTTCA CTCACAGCTA CCCTTTCTGC
    TGGAGGTCAG ACACGCCCCT CATTTACAAG TCTGTGCCCA GCTGGTTTGT GCGGGTGGAG
    CCCATGGTGG ACCAGCTACT GAAGAACAAC GACCTGTGCT ACTGGGTCCC AGAGTTTGTA
    CGAGAAAAAC GGTTTGGAAA TTGGCTGAAG GAGGCCCGTG ACTGGGCAAT TTCCAGAAAC
    AGATACTGGG GCACCCCCAT CCCACTGTGG GTCAGCGAAG ACTTGGAGGA GGTAGTGTGC
    ATTGGATCAG TGGCAGAGCT TGAAGAACTG TCGGGAACAA AAATCTCAGA TCTCCACAGA
    GAGAGCATCG ACCACCTGAC CATCCCATCC CGCTGCGGGA AAGCACCATT GCGGCGCGTC
    TCTGAGGTGT TTGACTGCTG GTTCGAGAGT GGAAGTATGC CCTATGCCCA GGTCCATTAT
    CCGTTCGAGA GCAAGCGCGA GTTTGAGGAT GCCTTTCCTG CAGACTTCAT TGCTGAAGGC
    ATCGATCAAA CCAGAGGATG GTTTTACACC CTGCTGGTGC TAGCCACAGC GCTCTTTGGA
    CAGCCACCTT TCAAGAATGT GATTGTGAAT GGCCTCATCT TGGCAAGTGA TGGCCAAAAA
    ATGAGCAAGC GGAAAAAGAA CTACCCAGAT CCAGTTTCCA TCATCGATAA GTATGGTGCG
    GACGCCCTCA GGTTATATCT GATCAATTCC CCTGTGGTAA GAGCAGAAAA CCTGCGCTTC
    AAGGAGGAAG GTGTGCGGGA TGTCCTCAAA GATGTGCTGC TCCCGTGGTA CAACGCTTAC
    CGCTTCTTCA TCCAGAACGT CTTTAGGCTC CACAAGGAAG AAGAAGTGAA GTTCCTATAC
    AACGAGCATA CAGTCAGAGA AAGCCCTAAC ATCACCGACC GCTGGGTTCT GTCCTTCATG
    CAGTCGCTCC TTGGCTTCTT TGAGACCGAA ATGGCAGCTT ACAGGCTGTA CACTGTGGTA
    CCTCGCCTGG TCAAGTTTGT AGATATTCTA ACCAACTGGT ATGTCCGAAT GAACAGGAGG
    AGGTTAAAGG GTGAGAGTGG GGTGGAAGAC TGTGTCATGG CTCTGGAGAC ACTGTTCAGT
    GTCCTGCTTT CTCTGTGCAG ACTGATGGCC CCTTACACGC CCTTTCTGAC TGAGCTGATG
    TACCAGAATC TAAAGCTGCT GATTGATCCT GCTTCTCTCC GGGACAAGGA CACACTCAGC
    ATCCACTACC TCATGCTGCC TCGAGTTCGA GAGGAGCTGA TTGACAAGAA AACTGAGAAC
    GCCGTGTCTA GGATGCAGTC TGTGATCGAG CTTGGAAGAG TGATACGAGA CCGAAAAACT
    ATTCCAATAA AGTATCCTCT GAAGGAAATT GTGGTCATCC ACCAAGACCC AGAAGCTCTA
    GAGGATATCA GGTCCTTGGA GAAGTATATC ATTGAGGAAT TAAATGTTCG AAAAGTCACG
    CTGTCTACTG ATAAAAACAA GTACGGTATC CGACTAAGGG CTGAACCAGA TCACATGGTC
    CTTGGGAAGC GCCTGAAGGG GGCCTTCAAA GCGGTGATGA TGGCCATAAA GCGGCTGAGC
    AATGAGGAGC TGGAGCGGTT TCAGAAGAGC GGGAGCATTG TTGTGGAAGG CCACGAACTG
    CATGAGGAAG ACATTCGCCT CATGTACACC TTTGACCAGG CCACGGGAGG GACCGCGCAG
    TTTGAAGCAC ACTCTGATGC TCAGGCTCTA GTTCTCTTAG ATGTCACACC TGACCAGTCA
    ATGGTGGATG AGGGCATGGC TCGGGAAGTC ATCAATCGCA TCCAGAAGCT TCGAAAGAAG
    TGCAATCTGG TTCCAACGGA TGAAATAACA GTTTACTATA ATGCAAAATC TGAAGGAAGA
    TACTTGAATA ATGTCATTGA AAGCCACACT GACTTCATAT TTGCCACAAT CAAAGCTCCC
    TTGAAGCCAT ATCCAGTTCC AACATCAGAT AATATCCTTA TTCAAGAGCA AACACAGTTA
    AAGGGGTCCG AGCTGGAAAT CACGCTCACC AAAGGATCGT GTGTGCCTGG CCCTGCTTGT
    GCTTATGTGA ACCTTAACAT TTGTGCAAAT GGCACCGAGC AAGGTGGAGT GTTGCTCCTG
    GAGAATCCGA AAGGTGATAA TCAGCTGAAC CTGGTGAAAC TGAAGACTGT TGTTACCAGC
    GTATTTGGTG TTAAAAATGC AAAGCTGTCA GTCTTCCACG GTGAAACAGA AATACAGAAC
    CAAACTGACT TGCTGAGCCT CAGTGGGAGG ACGCTGTGTG TGACTGCAGG GGCATCCCCG
    TCTCCCATCA GCAGCCCCAG CACCCTGCTG TGTCAGTACC TCAACCTGCA GCTCCTGAAC
    GCAGAGCCAC AAGAGTGTTT AACAGGAACT GTGGGCACCC TCCTGCTTGA AAACCCACTC
    GGACAGAACG GCCTCACCCA CCAAGGGCTT GTGCATGAAG CAGCCAAGGT GTTCGGACTG
    AGGAGCAGGA GGCTAAGGCT GTTCCTGAAT GAGACTCAAA CACAAGAAAT TACAGAAGAT
    ATCCCAATGA AGACTTTGAA TATGAAGACT GTATATGTTT CTGTCTTGCC AACAACTGCA
    GATGGCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu03174
    Clone ID Related Accession (Same CDS sequence) XM_006516826.1 , XM_006516826.2 , NM_172015.3
    Accession Version XM_006516826.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3789bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product isoleucine--tRNA ligase, cytoplasmic isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000079.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006516826.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGGTTCAAC AAGTTCCAGA AAACATCAGT TTTCCTGCTG AAGAAGAAAA AATCTTGGAG 
    TTCTGGTCCA AACATAACTG TTTCCAGGAA TGCCTCAAAC AGTCAAAACT CAGGCCAAAG
    TTTACCTTCT ATGATGGGCC TCCCTTTGCT ACTGGACTGC CTCACTATGG ACACATACTT
    GCTGGGACAA TCAAAGATAT CGTTACGAGA TACGCTCACC AGAGCGGGTT TCACGTTGAC
    AGAAGATTCG GGTGGGACTG TCACGGCTTA CCTGTGGAGT ACGAAATCGA TAAGACACTG
    GGCATCAAAG GCCCGGAGGA TGTGGCCAAA ATGGGGATTG CAGAGTACAA CAAGCAGTGC
    CGAGCAATCG TGATGAGATA CTCCGCTGAG TGGAAGTCTA CTGTTACCAG ACTCGGCCGA
    TGGATCGACT TTGACAATGA CTACAAAACA CTATACCCAC AGTTCATGGA ATCAGTCTGG
    TGGGTCTTCA AGCAGCTGTA TGACAAAGGC CTCGTGTACA GAGGCGTGAA GGTCATGCCC
    TTCTCCACCG CGTGTGGCAC TCCACTCTCC AACTTCGAAT CTAACCAGAA TTACAAGGAT
    GTTCAGGATC CCTCAGTATT TGTAACATTC CCTTTGGAGG AAGATGAAAA TACATCCTTA
    GTTGCTTGGA CAACCACTCC TTGGACTCTA CCCAGTAACC TTGCACTATG CGTCAATCCA
    GAAATACAGT ATGTGAAGAT CAAAGATGTG GCCAGGGGAA AATTATTCAT CTTAACGGAA
    GCCAGATTAT CGGCCCTCTA TAAGCAAGAG AGCGACTATG AGATACTTGA AAGGTTTCCT
    GGTGCCTCTC TCAAAGGCAA GAAATACAAG CCGTTGTTTG ACTACTTTAT TAAGTGTAAG
    GAGAACGGTG CCTTCACCGT GCTTGTCGAC CACTATGTGA AGGATGAAGA AGGCACAGGC
    GTTGTGCACC AGGCTCCTTA CTTTGGTGCT GATGATCATC GTGTCTGTAT GGACTTCAAC
    ATTATTCAGA AAGACTCAGT CCCTGTTTGT CCTGTGGATG CTTCAGGCTG CTTCACAGAG
    GAAGTGACAC ATTTTGTGGG GCAGTATGTG AAGGATGCTG ACAAAAATAT CATTAGGATG
    TTGAAGGAGC AGGGCCGGCT GCTGGCGGCG GGCACGTTCA CTCACAGCTA CCCTTTCTGC
    TGGAGGTCAG ACACGCCCCT CATTTACAAG TCTGTGCCCA GCTGGTTTGT GCGGGTGGAG
    CCCATGGTGG ACCAGCTACT GAAGAACAAC GACCTGTGCT ACTGGGTCCC AGAGTTTGTA
    CGAGAAAAAC GGTTTGGAAA TTGGCTGAAG GAGGCCCGTG ACTGGGCAAT TTCCAGAAAC
    AGATACTGGG GCACCCCCAT CCCACTGTGG GTCAGCGAAG ACTTGGAGGA GGTAGTGTGC
    ATTGGATCAG TGGCAGAGCT TGAAGAACTG TCGGGAACAA AAATCTCAGA TCTCCACAGA
    GAGAGCATCG ACCACCTGAC CATCCCATCC CGCTGCGGGA AAGCACCATT GCGGCGCGTC
    TCTGAGGTGT TTGACTGCTG GTTCGAGAGT GGAAGTATGC CCTATGCCCA GGTCCATTAT
    CCGTTCGAGA GCAAGCGCGA GTTTGAGGAT GCCTTTCCTG CAGACTTCAT TGCTGAAGGC
    ATCGATCAAA CCAGAGGATG GTTTTACACC CTGCTGGTGC TAGCCACAGC GCTCTTTGGA
    CAGCCACCTT TCAAGAATGT GATTGTGAAT GGCCTCATCT TGGCAAGTGA TGGCCAAAAA
    ATGAGCAAGC GGAAAAAGAA CTACCCAGAT CCAGTTTCCA TCATCGATAA GTATGGTGCG
    GACGCCCTCA GGTTATATCT GATCAATTCC CCTGTGGTAA GAGCAGAAAA CCTGCGCTTC
    AAGGAGGAAG GTGTGCGGGA TGTCCTCAAA GATGTGCTGC TCCCGTGGTA CAACGCTTAC
    CGCTTCTTCA TCCAGAACGT CTTTAGGCTC CACAAGGAAG AAGAAGTGAA GTTCCTATAC
    AACGAGCATA CAGTCAGAGA AAGCCCTAAC ATCACCGACC GCTGGGTTCT GTCCTTCATG
    CAGTCGCTCC TTGGCTTCTT TGAGACCGAA ATGGCAGCTT ACAGGCTGTA CACTGTGGTA
    CCTCGCCTGG TCAAGTTTGT AGATATTCTA ACCAACTGGT ATGTCCGAAT GAACAGGAGG
    AGGTTAAAGG GTGAGAGTGG GGTGGAAGAC TGTGTCATGG CTCTGGAGAC ACTGTTCAGT
    GTCCTGCTTT CTCTGTGCAG ACTGATGGCC CCTTACACGC CCTTTCTGAC TGAGCTGATG
    TACCAGAATC TAAAGCTGCT GATTGATCCT GCTTCTCTCC GGGACAAGGA CACACTCAGC
    ATCCACTACC TCATGCTGCC TCGAGTTCGA GAGGAGCTGA TTGACAAGAA AACTGAGAAC
    GCCGTGTCTA GGATGCAGTC TGTGATCGAG CTTGGAAGAG TGATACGAGA CCGAAAAACT
    ATTCCAATAA AGTATCCTCT GAAGGAAATT GTGGTCATCC ACCAAGACCC AGAAGCTCTA
    GAGGATATCA GGTCCTTGGA GAAGTATATC ATTGAGGAAT TAAATGTTCG AAAAGTCACG
    CTGTCTACTG ATAAAAACAA GTACGGTATC CGACTAAGGG CTGAACCAGA TCACATGGTC
    CTTGGGAAGC GCCTGAAGGG GGCCTTCAAA GCGGTGATGA TGGCCATAAA GCGGCTGAGC
    AATGAGGAGC TGGAGCGGTT TCAGAAGAGC GGGAGCATTG TTGTGGAAGG CCACGAACTG
    CATGAGGAAG ACATTCGCCT CATGTACACC TTTGACCAGG CCACGGGAGG GACCGCGCAG
    TTTGAAGCAC ACTCTGATGC TCAGGCTCTA GTTCTCTTAG ATGTCACACC TGACCAGTCA
    ATGGTGGATG AGGGCATGGC TCGGGAAGTC ATCAATCGCA TCCAGAAGCT TCGAAAGAAG
    TGCAATCTGG TTCCAACGGA TGAAATAACA GTTTACTATA ATGCAAAATC TGAAGGAAGA
    TACTTGAATA ATGTCATTGA AAGCCACACT GACTTCATAT TTGCCACAAT CAAAGCTCCC
    TTGAAGCCAT ATCCAGTTCC AACATCAGAT AATATCCTTA TTCAAGAGCA AACACAGTTA
    AAGGGGTCCG AGCTGGAAAT CACGCTCACC AAAGGATCGT GTGTGCCTGG CCCTGCTTGT
    GCTTATGTGA ACCTTAACAT TTGTGCAAAT GGCACCGAGC AAGGTGGAGT GTTGCTCCTG
    GAGAATCCGA AAGGTGATAA TCAGCTGAAC CTGGTGAAAC TGAAGACTGT TGTTACCAGC
    GTATTTGGTG TTAAAAATGC AAAGCTGTCA GTCTTCCACG GTGAAACAGA AATACAGAAC
    CAAACTGACT TGCTGAGCCT CAGTGGGAGG ACGCTGTGTG TGACTGCAGG GGCATCCCCG
    TCTCCCATCA GCAGCCCCAG CACCCTGCTG TGTCAGTACC TCAACCTGCA GCTCCTGAAC
    GCAGAGCCAC AAGAGTGTTT AACAGGAACT GTGGGCACCC TCCTGCTTGA AAACCCACTC
    GGACAGAACG GCCTCACCCA CCAAGGGCTT GTGCATGAAG CAGCCAAGGT GTTCGGACTG
    AGGAGCAGGA GGCTAAGGCT GTTCCTGAAT GAGACTCAAA CACAAGAAAT TACAGAAGAT
    ATCCCAATGA AGACTTTGAA TATGAAGACT GTATATGTTT CTGTCTTGCC AACAACTGCA
    GATGGCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006516889.1
    CDS126..3914
    Translation

    Target ORF information:

    RefSeq Version XM_006516826.2
    Organism Mus musculus(house mouse)
    Definition Mus musculus isoleucine-tRNA synthetase (Iars), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006516826.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGGTTCAAC AAGTTCCAGA AAACATCAGT TTTCCTGCTG AAGAAGAAAA AATCTTGGAG 
    TTCTGGTCCA AACATAACTG TTTCCAGGAA TGCCTCAAAC AGTCAAAACT CAGGCCAAAG
    TTTACCTTCT ATGATGGGCC TCCCTTTGCT ACTGGACTGC CTCACTATGG ACACATACTT
    GCTGGGACAA TCAAAGATAT CGTTACGAGA TACGCTCACC AGAGCGGGTT TCACGTTGAC
    AGAAGATTCG GGTGGGACTG TCACGGCTTA CCTGTGGAGT ACGAAATCGA TAAGACACTG
    GGCATCAAAG GCCCGGAGGA TGTGGCCAAA ATGGGGATTG CAGAGTACAA CAAGCAGTGC
    CGAGCAATCG TGATGAGATA CTCCGCTGAG TGGAAGTCTA CTGTTACCAG ACTCGGCCGA
    TGGATCGACT TTGACAATGA CTACAAAACA CTATACCCAC AGTTCATGGA ATCAGTCTGG
    TGGGTCTTCA AGCAGCTGTA TGACAAAGGC CTCGTGTACA GAGGCGTGAA GGTCATGCCC
    TTCTCCACCG CGTGTGGCAC TCCACTCTCC AACTTCGAAT CTAACCAGAA TTACAAGGAT
    GTTCAGGATC CCTCAGTATT TGTAACATTC CCTTTGGAGG AAGATGAAAA TACATCCTTA
    GTTGCTTGGA CAACCACTCC TTGGACTCTA CCCAGTAACC TTGCACTATG CGTCAATCCA
    GAAATACAGT ATGTGAAGAT CAAAGATGTG GCCAGGGGAA AATTATTCAT CTTAACGGAA
    GCCAGATTAT CGGCCCTCTA TAAGCAAGAG AGCGACTATG AGATACTTGA AAGGTTTCCT
    GGTGCCTCTC TCAAAGGCAA GAAATACAAG CCGTTGTTTG ACTACTTTAT TAAGTGTAAG
    GAGAACGGTG CCTTCACCGT GCTTGTCGAC CACTATGTGA AGGATGAAGA AGGCACAGGC
    GTTGTGCACC AGGCTCCTTA CTTTGGTGCT GATGATCATC GTGTCTGTAT GGACTTCAAC
    ATTATTCAGA AAGACTCAGT CCCTGTTTGT CCTGTGGATG CTTCAGGCTG CTTCACAGAG
    GAAGTGACAC ATTTTGTGGG GCAGTATGTG AAGGATGCTG ACAAAAATAT CATTAGGATG
    TTGAAGGAGC AGGGCCGGCT GCTGGCGGCG GGCACGTTCA CTCACAGCTA CCCTTTCTGC
    TGGAGGTCAG ACACGCCCCT CATTTACAAG TCTGTGCCCA GCTGGTTTGT GCGGGTGGAG
    CCCATGGTGG ACCAGCTACT GAAGAACAAC GACCTGTGCT ACTGGGTCCC AGAGTTTGTA
    CGAGAAAAAC GGTTTGGAAA TTGGCTGAAG GAGGCCCGTG ACTGGGCAAT TTCCAGAAAC
    AGATACTGGG GCACCCCCAT CCCACTGTGG GTCAGCGAAG ACTTGGAGGA GGTAGTGTGC
    ATTGGATCAG TGGCAGAGCT TGAAGAACTG TCGGGAACAA AAATCTCAGA TCTCCACAGA
    GAGAGCATCG ACCACCTGAC CATCCCATCC CGCTGCGGGA AAGCACCATT GCGGCGCGTC
    TCTGAGGTGT TTGACTGCTG GTTCGAGAGT GGAAGTATGC CCTATGCCCA GGTCCATTAT
    CCGTTCGAGA GCAAGCGCGA GTTTGAGGAT GCCTTTCCTG CAGACTTCAT TGCTGAAGGC
    ATCGATCAAA CCAGAGGATG GTTTTACACC CTGCTGGTGC TAGCCACAGC GCTCTTTGGA
    CAGCCACCTT TCAAGAATGT GATTGTGAAT GGCCTCATCT TGGCAAGTGA TGGCCAAAAA
    ATGAGCAAGC GGAAAAAGAA CTACCCAGAT CCAGTTTCCA TCATCGATAA GTATGGTGCG
    GACGCCCTCA GGTTATATCT GATCAATTCC CCTGTGGTAA GAGCAGAAAA CCTGCGCTTC
    AAGGAGGAAG GTGTGCGGGA TGTCCTCAAA GATGTGCTGC TCCCGTGGTA CAACGCTTAC
    CGCTTCTTCA TCCAGAACGT CTTTAGGCTC CACAAGGAAG AAGAAGTGAA GTTCCTATAC
    AACGAGCATA CAGTCAGAGA AAGCCCTAAC ATCACCGACC GCTGGGTTCT GTCCTTCATG
    CAGTCGCTCC TTGGCTTCTT TGAGACCGAA ATGGCAGCTT ACAGGCTGTA CACTGTGGTA
    CCTCGCCTGG TCAAGTTTGT AGATATTCTA ACCAACTGGT ATGTCCGAAT GAACAGGAGG
    AGGTTAAAGG GTGAGAGTGG GGTGGAAGAC TGTGTCATGG CTCTGGAGAC ACTGTTCAGT
    GTCCTGCTTT CTCTGTGCAG ACTGATGGCC CCTTACACGC CCTTTCTGAC TGAGCTGATG
    TACCAGAATC TAAAGCTGCT GATTGATCCT GCTTCTCTCC GGGACAAGGA CACACTCAGC
    ATCCACTACC TCATGCTGCC TCGAGTTCGA GAGGAGCTGA TTGACAAGAA AACTGAGAAC
    GCCGTGTCTA GGATGCAGTC TGTGATCGAG CTTGGAAGAG TGATACGAGA CCGAAAAACT
    ATTCCAATAA AGTATCCTCT GAAGGAAATT GTGGTCATCC ACCAAGACCC AGAAGCTCTA
    GAGGATATCA GGTCCTTGGA GAAGTATATC ATTGAGGAAT TAAATGTTCG AAAAGTCACG
    CTGTCTACTG ATAAAAACAA GTACGGTATC CGACTAAGGG CTGAACCAGA TCACATGGTC
    CTTGGGAAGC GCCTGAAGGG GGCCTTCAAA GCGGTGATGA TGGCCATAAA GCGGCTGAGC
    AATGAGGAGC TGGAGCGGTT TCAGAAGAGC GGGAGCATTG TTGTGGAAGG CCACGAACTG
    CATGAGGAAG ACATTCGCCT CATGTACACC TTTGACCAGG CCACGGGAGG GACCGCGCAG
    TTTGAAGCAC ACTCTGATGC TCAGGCTCTA GTTCTCTTAG ATGTCACACC TGACCAGTCA
    ATGGTGGATG AGGGCATGGC TCGGGAAGTC ATCAATCGCA TCCAGAAGCT TCGAAAGAAG
    TGCAATCTGG TTCCAACGGA TGAAATAACA GTTTACTATA ATGCAAAATC TGAAGGAAGA
    TACTTGAATA ATGTCATTGA AAGCCACACT GACTTCATAT TTGCCACAAT CAAAGCTCCC
    TTGAAGCCAT ATCCAGTTCC AACATCAGAT AATATCCTTA TTCAAGAGCA AACACAGTTA
    AAGGGGTCCG AGCTGGAAAT CACGCTCACC AAAGGATCGT GTGTGCCTGG CCCTGCTTGT
    GCTTATGTGA ACCTTAACAT TTGTGCAAAT GGCACCGAGC AAGGTGGAGT GTTGCTCCTG
    GAGAATCCGA AAGGTGATAA TCAGCTGAAC CTGGTGAAAC TGAAGACTGT TGTTACCAGC
    GTATTTGGTG TTAAAAATGC AAAGCTGTCA GTCTTCCACG GTGAAACAGA AATACAGAAC
    CAAACTGACT TGCTGAGCCT CAGTGGGAGG ACGCTGTGTG TGACTGCAGG GGCATCCCCG
    TCTCCCATCA GCAGCCCCAG CACCCTGCTG TGTCAGTACC TCAACCTGCA GCTCCTGAAC
    GCAGAGCCAC AAGAGTGTTT AACAGGAACT GTGGGCACCC TCCTGCTTGA AAACCCACTC
    GGACAGAACG GCCTCACCCA CCAAGGGCTT GTGCATGAAG CAGCCAAGGT GTTCGGACTG
    AGGAGCAGGA GGCTAAGGCT GTTCCTGAAT GAGACTCAAA CACAAGAAAT TACAGAAGAT
    ATCCCAATGA AGACTTTGAA TATGAAGACT GTATATGTTT CTGTCTTGCC AACAACTGCA
    GATGGCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.