Smg6 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Smg6
Entrez Gene ID	103677
Full Name	Smg-6 homolog, nonsense mediated mRNA decay factor (C. elegans)
Synonyms	AI317223,AU041178
General protein information	Preferred Names Smg-6 homolog, nonsense mediated mRNA decay factor (C. elegans) Names telomerase-binding protein EST1A EST1-like protein A ever shorter telomeres 1A telomerase subunit EST1A
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 11 Map Location: 11\|11 B5

mRNA and Protein(s)

mRNA	Protein	Name
XM_011248660.1	XP_011246962.1	telomerase-binding protein EST1A isoform X2
NM_001002764.2	NP_001002764.1	telomerase-binding protein EST1A
XM_011248659.3	XP_011246961.1	telomerase-binding protein EST1A isoform X1
XM_011248660.2	XP_011246962.1	telomerase-binding protein EST1A isoform X2
NM_001002764.1	NP_001002764.1	telomerase-binding protein EST1A
XM_030245450.1	XP_030101310.1	telomerase-binding protein EST1A isoform X2
XM_011248659.2	XP_011246961.1	telomerase-binding protein EST1A isoform X1

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	SMG6	XP_548319.3
	LOC721291	XP_002800291.1
Gallus gallus (chicken)	LOC101750600	XP_004946606.1
Homo sapiens (human)	SMG6	NP_060045.4
Danio rerio (zebrafish)	LOC100334020	XP_005172798.1
Pan troglodytes (chimpanzee)	SMG6	XP_003315338.1
Bos taurus (cattle)	SMG6	NP_001192756.1
Rattus norvegicus (Norway rat)	Smg6	NP_001099278.1
Xenopus tropicalis (tropical clawed frog)	smg6	XP_002937186.2
Mus musculus (house mouse)	Smg6	NP_001002764.1

Gene Ontology
Bibliography

Related articles in PubMed

Smg6/Est1 licenses embryonic stem cell differentiation via nonsense-mediated mRNA decay.
Li T, Shi Y, Wang P, Guachalla LM, Sun B, Joerss T, Chen YS, Groth M, Krueger A, Platzer M, Yang YG, Rudolph KL, Wang ZQ
The EMBO journal34(12)1630-47(2015 Jun)

Characterization of an exchangeable gene trap using pU-17 carrying a stop codon-beta geo cassette.
Taniwaki T, Haruna K, Nakamura H, Sekimoto T, Oike Y, Imaizumi T, Saito F, Muta M, Soejima Y, Utoh A, Nakagata N, Araki M, Yamamura K, Araki K
Development, growth & differentiation47(3)163-72(2005 Apr)

Tagging genes with cassette-exchange sites.
Cobellis G, Nicolaus G, Iovino M, Romito A, Marra E, Barbarisi M, Sardiello M, Di Giorgio FP, Iovino N, Zollo M, Ballabio A, Cortese R
Nucleic acids research33(4)e44(2005 Mar)

Prediction of the coding sequences of mouse homologues of KIAA gene: III. the complete nucleotide sequences of 500 mouse KIAA-homologous cDNAs identified by screening of terminal sequences of cDNA clones randomly sampled from size-fractionated libraries.
Okazaki N, Kikuno R, Ohara R, Inamoto S, Koseki H, Hiraoka S, Saga Y, Nagase T, Ohara O, Koga H
DNA research : an international journal for rapid publication of reports on genes and genomes10(4)167-80(2003 Aug)

BayGenomics: a resource of insertional mutations in mouse embryonic stem cells.
Stryke D, Kawamoto M, Huang CC, Johns SJ, King LA, Harper CA, Meng EC, Lee RE, Yee A, L'Italien L, Chuang PT, Young SG, Skarnes WC, Babbitt PC, Ferrin TE
Nucleic acids research31(1)278-81(2003 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Smg6/Est1 licenses embryonic stem cell differentiation via nonsense-mediated mRNA decay.
Title: Smg6/Est1 licenses embryonic stem cell differentiation via nonsense-mediated mRNA decay.

The following Smg6 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Smg6 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu72063	XM_030245450.1 Latest version!	Mus musculus Smg-6 homolog, nonsense mediated mRNA decay factor (C. elegans) (Smg6), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
OMu72063	XM_011248660.2 Latest version!	Mus musculus Smg-6 homolog, nonsense mediated mRNA decay factor (C. elegans) (Smg6), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
OMu14550	NM_001002764.2 Latest version!	Mus musculus Smg-6 homolog, nonsense mediated mRNA decay factor (C. elegans) (Smg6), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	16	$832.30 $1189.00
OMu72062	XM_011248659.3 Latest version!	Mus musculus Smg-6 homolog, nonsense mediated mRNA decay factor (C. elegans) (Smg6), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
View Old Accession Versions >>
OMu72063	XM_011248660.1	Mus musculus Smg-6 homolog, nonsense mediated mRNA decay factor (C. elegans) (Smg6), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$307.30 $439.00
OMu14550	NM_001002764.1	Mus musculus Smg-6 homolog, nonsense mediated mRNA decay factor (C. elegans) (Smg6), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	16	$832.30 $1189.00
OMu72062	XM_011248659.2	Mus musculus Smg-6 homolog, nonsense mediated mRNA decay factor (C. elegans) (Smg6), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$727.30 $1039.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Smg6 ( XM_011248660.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011246962.1 Mus musculus Smg-6 homolog, nonsense mediated mRNA decay factor (C. elegans) (Smg6), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OMu72063
Clone ID Related Accession (Same CDS sequence)	XM_011248660.1 , XM_011248660.2 , XM_030245450.1
Accession Version	XM_011248660.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1536bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	telomerase-binding protein EST1A isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000077.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGACAT TCCCTGCAGT GGCTGAGAAG GTCCTTAAGG AGTTCCAAGT ATTGCTGCAG 
CATAGCCCAT CTCCTATTGG AAGTACCCGT ATGCTGCAGC TTATGACCAT CAACATGTTT 
GCCGTGCATA ACTCCCAGTT GAAAGACTGC TTCTCAGAGG AGTGTCGTTC TGTGATCCAG 
GAACAAGCTG CATCCTTGGG CTTGGCTATG TTTTCTCTAC TGGTCCAGCG CTGCACATGC 
CTACTTAAGG ACTCTGCTAA AGCTCAGCTG TCCTCTCCTG AGGACCAAGA GGACCAAGAT 
GACATCAAGG TATCTTCCTT CGTCCCGGAC CTGAAGGAGC TGCTCCCCAG TGTGAAAGTC 
TGGTCTGATT GGATGCTTGG CTACCCAGAC ACCTGGAATC CTCCACCCAC ATCTTTGGAT 
CTGCCCTTGC AGGTTGCTGT GGATGTCTGG TCAACACTAG CTGATTTCTG TAACATACTG 
ACTGCAGTCA ATCAGTCTGA GGTGCCACTG TACAAGGACC CAGATGATGA CCTCACCCTG 
CTTATCCTGG AAGAGGATCG GCTTCTCTCT GGCTTTGTCC CCTTGCTGGC TGCCCCTCAG 
GACCCCTGCT ACGTGGAGAA AACCTCGGAT AAGGTTATTG CAGCTGACTG CAAAAGGGTC 
ACAGTGCTGA AGTATTTTCT GGAAGCCCTT TGTGGACAAG AAGAGCCTCT GCTGGCATTC 
AAGGGTGGAA AATATGTGTC AGTGGCACCC GTCCCAGACA CCATGGGAAA GGAAATGGGA 
AGCCAAGAGG GAAAACAACT GGAAGATGAG GAAGAAGATG TGGTGATTGA AGACTTTGAG 
GAAGATTCAG AGGCTGAAGG CAGTGGGGGT GAGGATGACA TCAGGGAACT TCGGGCCAAG 
AAGCTGGCTC TAGCAAGGAA GATAGCTGAG CAGCAGCGTC GCCAGGAAAA GATCCAGGCT 
GTTTTGGAAG ACCAGAGTCA GATGAGGCAG ATGGAGCTGG AGATCAGACC CTTGTTCCTC 
GTACCAGATA CCAACGGCTT CATTGACCAC CTGGCCAGTC TGGCTCGGCT GCTGGAGAGC 
AGGAAGTACA TCCTGGTGGT GCCCCTCATC GTGATCAATG AGCTGGACGG CCTCGCCAAA 
GGGCAGGAGA CAGACCACCG GGCTGGGGGC TATGCCCGTG TGGTGCAAGA AAAGGCCCGA 
AAATCCATTG AGTTCCTCGA ACGGAGATTT GAGAGTCGGG ACTCCTGCCT GCGGGCCTTG 
ACCAGCCGTG GCAATGAACT TGAATCTATC GCCTTCCGAA GTGAGGACAT TACTGGACAG 
CTGGGTAACA ACGATGACCT GATCCTCTCT TGCTGCCTGC ACTACTGCAA AGACAAGGCT 
AAGGATTACA TGCCCACCAG CAAAGAGGAG CCAATCCGCC TGTTACGGGA GGTGGTGTTG 
CTGACCGATG ATCGGAACCT TCGTGTAAAG GCGCTGACAA GGAATGTGCC TGTGAGGGAC 
ATCCCAGCCT TCCTTACGTG GGCCCAGGTG GGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011246962.1
CDS	138..1673
Translation	METFPAVAEKVLKEFQVLLQHSPSPIGSTRMLQLMTINMFAVHNSQLKDCFSEECRSVIQEQAASLGLAMFSLLVQRCTCLLKDSAKAQLSSPEDQEDQDDIKVSSFVPDLKELLPSVKVWSDWMLGYPDTWNPPPTSLDLPLQVAVDVWSTLADFCNILTAVNQSEVPLYKDPDDDLTLLILEEDRLLSGFVPLLAAPQDPCYVEKTSDKVIAADCKRVTVLKYFLEALCGQEEPLLAFKGGKYVSVAPVPDTMGKEMGSQEGKQLEDEEEDVVIEDFEEDSEAEGSGGEDDIRELRAKKLALARKIAEQQRRQEKIQAVLEDQSQMRQMELEIRPLFLVPDTNGFIDHLASLARLLESRKYILVVPLIVINELDGLAKGQETDHRAGGYARVVQEKARKSIEFLERRFESRDSCLRALTSRGNELESIAFRSEDITGQLGNNDDLILSCCLHYCKDKAKDYMPTSKEEPIRLLREVVLLTDDRNLRVKALTRNVPVRDIPAFLTWAQVG

Target ORF information:

RefSeq Version	XM_011248660.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus Smg-6 homolog, nonsense mediated mRNA decay factor (C. elegans) (Smg6), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011248660.1

ORF Insert Sequence:

ATGGAGACAT TCCCTGCAGT GGCTGAGAAG GTCCTTAAGG AGTTCCAAGT ATTGCTGCAG 
CATAGCCCAT CTCCTATTGG AAGTACCCGT ATGCTGCAGC TTATGACCAT CAACATGTTT 
GCCGTGCATA ACTCCCAGTT GAAAGACTGC TTCTCAGAGG AGTGTCGTTC TGTGATCCAG 
GAACAAGCTG CATCCTTGGG CTTGGCTATG TTTTCTCTAC TGGTCCAGCG CTGCACATGC 
CTACTTAAGG ACTCTGCTAA AGCTCAGCTG TCCTCTCCTG AGGACCAAGA GGACCAAGAT 
GACATCAAGG TATCTTCCTT CGTCCCGGAC CTGAAGGAGC TGCTCCCCAG TGTGAAAGTC 
TGGTCTGATT GGATGCTTGG CTACCCAGAC ACCTGGAATC CTCCACCCAC ATCTTTGGAT 
CTGCCCTTGC AGGTTGCTGT GGATGTCTGG TCAACACTAG CTGATTTCTG TAACATACTG 
ACTGCAGTCA ATCAGTCTGA GGTGCCACTG TACAAGGACC CAGATGATGA CCTCACCCTG 
CTTATCCTGG AAGAGGATCG GCTTCTCTCT GGCTTTGTCC CCTTGCTGGC TGCCCCTCAG 
GACCCCTGCT ACGTGGAGAA AACCTCGGAT AAGGTTATTG CAGCTGACTG CAAAAGGGTC 
ACAGTGCTGA AGTATTTTCT GGAAGCCCTT TGTGGACAAG AAGAGCCTCT GCTGGCATTC 
AAGGGTGGAA AATATGTGTC AGTGGCACCC GTCCCAGACA CCATGGGAAA GGAAATGGGA 
AGCCAAGAGG GAAAACAACT GGAAGATGAG GAAGAAGATG TGGTGATTGA AGACTTTGAG 
GAAGATTCAG AGGCTGAAGG CAGTGGGGGT GAGGATGACA TCAGGGAACT TCGGGCCAAG 
AAGCTGGCTC TAGCAAGGAA GATAGCTGAG CAGCAGCGTC GCCAGGAAAA GATCCAGGCT 
GTTTTGGAAG ACCAGAGTCA GATGAGGCAG ATGGAGCTGG AGATCAGACC CTTGTTCCTC 
GTACCAGATA CCAACGGCTT CATTGACCAC CTGGCCAGTC TGGCTCGGCT GCTGGAGAGC 
AGGAAGTACA TCCTGGTGGT GCCCCTCATC GTGATCAATG AGCTGGACGG CCTCGCCAAA 
GGGCAGGAGA CAGACCACCG GGCTGGGGGC TATGCCCGTG TGGTGCAAGA AAAGGCCCGA 
AAATCCATTG AGTTCCTCGA ACGGAGATTT GAGAGTCGGG ACTCCTGCCT GCGGGCCTTG 
ACCAGCCGTG GCAATGAACT TGAATCTATC GCCTTCCGAA GTGAGGACAT TACTGGACAG 
CTGGGTAACA ACGATGACCT GATCCTCTCT TGCTGCCTGC ACTACTGCAA AGACAAGGCT 
AAGGATTACA TGCCCACCAG CAAAGAGGAG CCAATCCGCC TGTTACGGGA GGTGGTGTTG 
CTGACCGATG ATCGGAACCT TCGTGTAAAG GCGCTGACAA GGAATGTGCC TGTGAGGGAC 
ATCCCAGCCT TCCTTACGTG GGCCCAGGTG GGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Smg6 ( XM_011248660.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011246962.1 Mus musculus Smg-6 homolog, nonsense mediated mRNA decay factor (C. elegans) (Smg6), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OMu72063
Clone ID Related Accession (Same CDS sequence)	XM_011248660.1 , XM_011248660.2 , XM_030245450.1
Accession Version	XM_011248660.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1536bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	telomerase-binding protein EST1A isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000077.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_011248660.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGACAT TCCCTGCAGT GGCTGAGAAG GTCCTTAAGG AGTTCCAAGT ATTGCTGCAG 
CATAGCCCAT CTCCTATTGG AAGTACCCGT ATGCTGCAGC TTATGACCAT CAACATGTTT 
GCCGTGCATA ACTCCCAGTT GAAAGACTGC TTCTCAGAGG AGTGTCGTTC TGTGATCCAG 
GAACAAGCTG CATCCTTGGG CTTGGCTATG TTTTCTCTAC TGGTCCAGCG CTGCACATGC 
CTACTTAAGG ACTCTGCTAA AGCTCAGCTG TCCTCTCCTG AGGACCAAGA GGACCAAGAT 
GACATCAAGG TATCTTCCTT CGTCCCGGAC CTGAAGGAGC TGCTCCCCAG TGTGAAAGTC 
TGGTCTGATT GGATGCTTGG CTACCCAGAC ACCTGGAATC CTCCACCCAC ATCTTTGGAT 
CTGCCCTTGC AGGTTGCTGT GGATGTCTGG TCAACACTAG CTGATTTCTG TAACATACTG 
ACTGCAGTCA ATCAGTCTGA GGTGCCACTG TACAAGGACC CAGATGATGA CCTCACCCTG 
CTTATCCTGG AAGAGGATCG GCTTCTCTCT GGCTTTGTCC CCTTGCTGGC TGCCCCTCAG 
GACCCCTGCT ACGTGGAGAA AACCTCGGAT AAGGTTATTG CAGCTGACTG CAAAAGGGTC 
ACAGTGCTGA AGTATTTTCT GGAAGCCCTT TGTGGACAAG AAGAGCCTCT GCTGGCATTC 
AAGGGTGGAA AATATGTGTC AGTGGCACCC GTCCCAGACA CCATGGGAAA GGAAATGGGA 
AGCCAAGAGG GAAAACAACT GGAAGATGAG GAAGAAGATG TGGTGATTGA AGACTTTGAG 
GAAGATTCAG AGGCTGAAGG CAGTGGGGGT GAGGATGACA TCAGGGAACT TCGGGCCAAG 
AAGCTGGCTC TAGCAAGGAA GATAGCTGAG CAGCAGCGTC GCCAGGAAAA GATCCAGGCT 
GTTTTGGAAG ACCAGAGTCA GATGAGGCAG ATGGAGCTGG AGATCAGACC CTTGTTCCTC 
GTACCAGATA CCAACGGCTT CATTGACCAC CTGGCCAGTC TGGCTCGGCT GCTGGAGAGC 
AGGAAGTACA TCCTGGTGGT GCCCCTCATC GTGATCAATG AGCTGGACGG CCTCGCCAAA 
GGGCAGGAGA CAGACCACCG GGCTGGGGGC TATGCCCGTG TGGTGCAAGA AAAGGCCCGA 
AAATCCATTG AGTTCCTCGA ACGGAGATTT GAGAGTCGGG ACTCCTGCCT GCGGGCCTTG 
ACCAGCCGTG GCAATGAACT TGAATCTATC GCCTTCCGAA GTGAGGACAT TACTGGACAG 
CTGGGTAACA ACGATGACCT GATCCTCTCT TGCTGCCTGC ACTACTGCAA AGACAAGGCT 
AAGGATTACA TGCCCACCAG CAAAGAGGAG CCAATCCGCC TGTTACGGGA GGTGGTGTTG 
CTGACCGATG ATCGGAACCT TCGTGTAAAG GCGCTGACAA GGAATGTGCC TGTGAGGGAC 
ATCCCAGCCT TCCTTACGTG GGCCCAGGTG GGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011246962.1
CDS	214..1749
Translation	METFPAVAEKVLKEFQVLLQHSPSPIGSTRMLQLMTINMFAVHNSQLKDCFSEECRSVIQEQAASLGLAMFSLLVQRCTCLLKDSAKAQLSSPEDQEDQDDIKVSSFVPDLKELLPSVKVWSDWMLGYPDTWNPPPTSLDLPLQVAVDVWSTLADFCNILTAVNQSEVPLYKDPDDDLTLLILEEDRLLSGFVPLLAAPQDPCYVEKTSDKVIAADCKRVTVLKYFLEALCGQEEPLLAFKGGKYVSVAPVPDTMGKEMGSQEGKQLEDEEEDVVIEDFEEDSEAEGSGGEDDIRELRAKKLALARKIAEQQRRQEKIQAVLEDQSQMRQMELEIRPLFLVPDTNGFIDHLASLARLLESRKYILVVPLIVINELDGLAKGQETDHRAGGYARVVQEKARKSIEFLERRFESRDSCLRALTSRGNELESIAFRSEDITGQLGNNDDLILSCCLHYCKDKAKDYMPTSKEEPIRLLREVVLLTDDRNLRVKALTRNVPVRDIPAFLTWAQVG

Target ORF information:

RefSeq Version	XM_011248660.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus Smg-6 homolog, nonsense mediated mRNA decay factor (C. elegans) (Smg6), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011248660.2

ORF Insert Sequence:

ATGGAGACAT TCCCTGCAGT GGCTGAGAAG GTCCTTAAGG AGTTCCAAGT ATTGCTGCAG 
CATAGCCCAT CTCCTATTGG AAGTACCCGT ATGCTGCAGC TTATGACCAT CAACATGTTT 
GCCGTGCATA ACTCCCAGTT GAAAGACTGC TTCTCAGAGG AGTGTCGTTC TGTGATCCAG 
GAACAAGCTG CATCCTTGGG CTTGGCTATG TTTTCTCTAC TGGTCCAGCG CTGCACATGC 
CTACTTAAGG ACTCTGCTAA AGCTCAGCTG TCCTCTCCTG AGGACCAAGA GGACCAAGAT 
GACATCAAGG TATCTTCCTT CGTCCCGGAC CTGAAGGAGC TGCTCCCCAG TGTGAAAGTC 
TGGTCTGATT GGATGCTTGG CTACCCAGAC ACCTGGAATC CTCCACCCAC ATCTTTGGAT 
CTGCCCTTGC AGGTTGCTGT GGATGTCTGG TCAACACTAG CTGATTTCTG TAACATACTG 
ACTGCAGTCA ATCAGTCTGA GGTGCCACTG TACAAGGACC CAGATGATGA CCTCACCCTG 
CTTATCCTGG AAGAGGATCG GCTTCTCTCT GGCTTTGTCC CCTTGCTGGC TGCCCCTCAG 
GACCCCTGCT ACGTGGAGAA AACCTCGGAT AAGGTTATTG CAGCTGACTG CAAAAGGGTC 
ACAGTGCTGA AGTATTTTCT GGAAGCCCTT TGTGGACAAG AAGAGCCTCT GCTGGCATTC 
AAGGGTGGAA AATATGTGTC AGTGGCACCC GTCCCAGACA CCATGGGAAA GGAAATGGGA 
AGCCAAGAGG GAAAACAACT GGAAGATGAG GAAGAAGATG TGGTGATTGA AGACTTTGAG 
GAAGATTCAG AGGCTGAAGG CAGTGGGGGT GAGGATGACA TCAGGGAACT TCGGGCCAAG 
AAGCTGGCTC TAGCAAGGAA GATAGCTGAG CAGCAGCGTC GCCAGGAAAA GATCCAGGCT 
GTTTTGGAAG ACCAGAGTCA GATGAGGCAG ATGGAGCTGG AGATCAGACC CTTGTTCCTC 
GTACCAGATA CCAACGGCTT CATTGACCAC CTGGCCAGTC TGGCTCGGCT GCTGGAGAGC 
AGGAAGTACA TCCTGGTGGT GCCCCTCATC GTGATCAATG AGCTGGACGG CCTCGCCAAA 
GGGCAGGAGA CAGACCACCG GGCTGGGGGC TATGCCCGTG TGGTGCAAGA AAAGGCCCGA 
AAATCCATTG AGTTCCTCGA ACGGAGATTT GAGAGTCGGG ACTCCTGCCT GCGGGCCTTG 
ACCAGCCGTG GCAATGAACT TGAATCTATC GCCTTCCGAA GTGAGGACAT TACTGGACAG 
CTGGGTAACA ACGATGACCT GATCCTCTCT TGCTGCCTGC ACTACTGCAA AGACAAGGCT 
AAGGATTACA TGCCCACCAG CAAAGAGGAG CCAATCCGCC TGTTACGGGA GGTGGTGTTG 
CTGACCGATG ATCGGAACCT TCGTGTAAAG GCGCTGACAA GGAATGTGCC TGTGAGGGAC 
ATCCCAGCCT TCCTTACGTG GGCCCAGGTG GGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Smg6 ( XM_030245450.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030101310.1 Mus musculus Smg-6 homolog, nonsense mediated mRNA decay factor (C. elegans) (Smg6), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OMu72063
Clone ID Related Accession (Same CDS sequence)	XM_011248660.1 , XM_011248660.2 , XM_030245450.1
Accession Version	XM_030245450.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1536bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	telomerase-binding protein EST1A isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000077.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGACAT TCCCTGCAGT GGCTGAGAAG GTCCTTAAGG AGTTCCAAGT ATTGCTGCAG 
CATAGCCCAT CTCCTATTGG AAGTACCCGT ATGCTGCAGC TTATGACCAT CAACATGTTT 
GCCGTGCATA ACTCCCAGTT GAAAGACTGC TTCTCAGAGG AGTGTCGTTC TGTGATCCAG 
GAACAAGCTG CATCCTTGGG CTTGGCTATG TTTTCTCTAC TGGTCCAGCG CTGCACATGC 
CTACTTAAGG ACTCTGCTAA AGCTCAGCTG TCCTCTCCTG AGGACCAAGA GGACCAAGAT 
GACATCAAGG TATCTTCCTT CGTCCCGGAC CTGAAGGAGC TGCTCCCCAG TGTGAAAGTC 
TGGTCTGATT GGATGCTTGG CTACCCAGAC ACCTGGAATC CTCCACCCAC ATCTTTGGAT 
CTGCCCTTGC AGGTTGCTGT GGATGTCTGG TCAACACTAG CTGATTTCTG TAACATACTG 
ACTGCAGTCA ATCAGTCTGA GGTGCCACTG TACAAGGACC CAGATGATGA CCTCACCCTG 
CTTATCCTGG AAGAGGATCG GCTTCTCTCT GGCTTTGTCC CCTTGCTGGC TGCCCCTCAG 
GACCCCTGCT ACGTGGAGAA AACCTCGGAT AAGGTTATTG CAGCTGACTG CAAAAGGGTC 
ACAGTGCTGA AGTATTTTCT GGAAGCCCTT TGTGGACAAG AAGAGCCTCT GCTGGCATTC 
AAGGGTGGAA AATATGTGTC AGTGGCACCC GTCCCAGACA CCATGGGAAA GGAAATGGGA 
AGCCAAGAGG GAAAACAACT GGAAGATGAG GAAGAAGATG TGGTGATTGA AGACTTTGAG 
GAAGATTCAG AGGCTGAAGG CAGTGGGGGT GAGGATGACA TCAGGGAACT TCGGGCCAAG 
AAGCTGGCTC TAGCAAGGAA GATAGCTGAG CAGCAGCGTC GCCAGGAAAA GATCCAGGCT 
GTTTTGGAAG ACCAGAGTCA GATGAGGCAG ATGGAGCTGG AGATCAGACC CTTGTTCCTC 
GTACCAGATA CCAACGGCTT CATTGACCAC CTGGCCAGTC TGGCTCGGCT GCTGGAGAGC 
AGGAAGTACA TCCTGGTGGT GCCCCTCATC GTGATCAATG AGCTGGACGG CCTCGCCAAA 
GGGCAGGAGA CAGACCACCG GGCTGGGGGC TATGCCCGTG TGGTGCAAGA AAAGGCCCGA 
AAATCCATTG AGTTCCTCGA ACGGAGATTT GAGAGTCGGG ACTCCTGCCT GCGGGCCTTG 
ACCAGCCGTG GCAATGAACT TGAATCTATC GCCTTCCGAA GTGAGGACAT TACTGGACAG 
CTGGGTAACA ACGATGACCT GATCCTCTCT TGCTGCCTGC ACTACTGCAA AGACAAGGCT 
AAGGATTACA TGCCCACCAG CAAAGAGGAG CCAATCCGCC TGTTACGGGA GGTGGTGTTG 
CTGACCGATG ATCGGAACCT TCGTGTAAAG GCGCTGACAA GGAATGTGCC TGTGAGGGAC 
ATCCCAGCCT TCCTTACGTG GGCCCAGGTG GGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030101310.1
CDS	71..1606
Translation	METFPAVAEKVLKEFQVLLQHSPSPIGSTRMLQLMTINMFAVHNSQLKDCFSEECRSVIQEQAASLGLAMFSLLVQRCTCLLKDSAKAQLSSPEDQEDQDDIKVSSFVPDLKELLPSVKVWSDWMLGYPDTWNPPPTSLDLPLQVAVDVWSTLADFCNILTAVNQSEVPLYKDPDDDLTLLILEEDRLLSGFVPLLAAPQDPCYVEKTSDKVIAADCKRVTVLKYFLEALCGQEEPLLAFKGGKYVSVAPVPDTMGKEMGSQEGKQLEDEEEDVVIEDFEEDSEAEGSGGEDDIRELRAKKLALARKIAEQQRRQEKIQAVLEDQSQMRQMELEIRPLFLVPDTNGFIDHLASLARLLESRKYILVVPLIVINELDGLAKGQETDHRAGGYARVVQEKARKSIEFLERRFESRDSCLRALTSRGNELESIAFRSEDITGQLGNNDDLILSCCLHYCKDKAKDYMPTSKEEPIRLLREVVLLTDDRNLRVKALTRNVPVRDIPAFLTWAQVG

Target ORF information:

RefSeq Version	XM_030245450.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus Smg-6 homolog, nonsense mediated mRNA decay factor (C. elegans) (Smg6), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030245450.1

ORF Insert Sequence:

ATGGAGACAT TCCCTGCAGT GGCTGAGAAG GTCCTTAAGG AGTTCCAAGT ATTGCTGCAG 
CATAGCCCAT CTCCTATTGG AAGTACCCGT ATGCTGCAGC TTATGACCAT CAACATGTTT 
GCCGTGCATA ACTCCCAGTT GAAAGACTGC TTCTCAGAGG AGTGTCGTTC TGTGATCCAG 
GAACAAGCTG CATCCTTGGG CTTGGCTATG TTTTCTCTAC TGGTCCAGCG CTGCACATGC 
CTACTTAAGG ACTCTGCTAA AGCTCAGCTG TCCTCTCCTG AGGACCAAGA GGACCAAGAT 
GACATCAAGG TATCTTCCTT CGTCCCGGAC CTGAAGGAGC TGCTCCCCAG TGTGAAAGTC 
TGGTCTGATT GGATGCTTGG CTACCCAGAC ACCTGGAATC CTCCACCCAC ATCTTTGGAT 
CTGCCCTTGC AGGTTGCTGT GGATGTCTGG TCAACACTAG CTGATTTCTG TAACATACTG 
ACTGCAGTCA ATCAGTCTGA GGTGCCACTG TACAAGGACC CAGATGATGA CCTCACCCTG 
CTTATCCTGG AAGAGGATCG GCTTCTCTCT GGCTTTGTCC CCTTGCTGGC TGCCCCTCAG 
GACCCCTGCT ACGTGGAGAA AACCTCGGAT AAGGTTATTG CAGCTGACTG CAAAAGGGTC 
ACAGTGCTGA AGTATTTTCT GGAAGCCCTT TGTGGACAAG AAGAGCCTCT GCTGGCATTC 
AAGGGTGGAA AATATGTGTC AGTGGCACCC GTCCCAGACA CCATGGGAAA GGAAATGGGA 
AGCCAAGAGG GAAAACAACT GGAAGATGAG GAAGAAGATG TGGTGATTGA AGACTTTGAG 
GAAGATTCAG AGGCTGAAGG CAGTGGGGGT GAGGATGACA TCAGGGAACT TCGGGCCAAG 
AAGCTGGCTC TAGCAAGGAA GATAGCTGAG CAGCAGCGTC GCCAGGAAAA GATCCAGGCT 
GTTTTGGAAG ACCAGAGTCA GATGAGGCAG ATGGAGCTGG AGATCAGACC CTTGTTCCTC 
GTACCAGATA CCAACGGCTT CATTGACCAC CTGGCCAGTC TGGCTCGGCT GCTGGAGAGC 
AGGAAGTACA TCCTGGTGGT GCCCCTCATC GTGATCAATG AGCTGGACGG CCTCGCCAAA 
GGGCAGGAGA CAGACCACCG GGCTGGGGGC TATGCCCGTG TGGTGCAAGA AAAGGCCCGA 
AAATCCATTG AGTTCCTCGA ACGGAGATTT GAGAGTCGGG ACTCCTGCCT GCGGGCCTTG 
ACCAGCCGTG GCAATGAACT TGAATCTATC GCCTTCCGAA GTGAGGACAT TACTGGACAG 
CTGGGTAACA ACGATGACCT GATCCTCTCT TGCTGCCTGC ACTACTGCAA AGACAAGGCT 
AAGGATTACA TGCCCACCAG CAAAGAGGAG CCAATCCGCC TGTTACGGGA GGTGGTGTTG 
CTGACCGATG ATCGGAACCT TCGTGTAAAG GCGCTGACAA GGAATGTGCC TGTGAGGGAC 
ATCCCAGCCT TCCTTACGTG GGCCCAGGTG GGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Smg6 ( NM_001002764.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001002764.1 Mus musculus Smg-6 homolog, nonsense mediated mRNA decay factor (C. elegans) (Smg6), mRNA.

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CloneID	OMu14550
Clone ID Related Accession (Same CDS sequence)	NM_001002764.2 , NM_001002764.1
Accession Version	NM_001002764.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4257bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-26
Organism	Mus musculus(house mouse)
Product	telomerase-binding protein EST1A
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BY109058.1 and BC066040.1. On Jul 4, 2018 this sequence version replaced NM_001002764.1. ##Evidence-Data-START## Transcript exon combination :: BC047279.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCGGAGG GGTTGGAGCG TGTGCGGATC TCCGCATCGG AACTGCGAGG GATCCTGGCC 
ACTCTGGCTC CGCAGGCCGG GAGCAGAGAA AACATGAAGG AACTAAAGGA ACCCAGACAG 
CGCAAAGATA ATAGGCGTCC AGATTTGGAA ATCTATAAGC CTGGACTTTC TCGACTAAGG 
AACAGACCTA AAACTAAGGA GGCTTCTGGG AATGAGGAAT TTAAGGATGA GATAGTAAAT 
GACAGAGATT CTTCTGCTGT TGGGAATGAT ACACAGCTTA TTCAAGTTTG CAAGGAATTG 
GACAGCCAAC AGCAAAATGG CCCTATAGAT GCAGAAAATA GTCAGGCACA GGAAACTTTT 
CCAAAGACTG TTGGACTAGA GGACCGAAGT CTAAAGATCA TCAAAAGAAG CAAGAAACCA 
GACCTGCAGA TCTACCAGCC TGGAAGGCGG TTGCAGACCA TTACCAAAGA GTCAGCAGGC 
AGGGCAGATG AAGAAGAGAT CCTCAACCAA GTGGAGCAGT TGAGGATAGA GGAGGATGAG 
TGTAAGGGAG AAGCTATAAA AGAAGAAGTT AATAACAAAC CGGATAAAAC CGAGATAGAA 
AAGCACCAAA GTAATGACCG TGTAAGAACT GCCAAGGGAG AAAAGGGAAA AAAGATTGAA 
AAGGGGGAGG GGTCAAAGAA GGTGGCTGAT GACTCTGTCC CAGGAAAGCC TGGCTCTGTA 
AAGAGATACT CGAGATCAGA CAAGAGAAGG AATCGCTACC GCACTTGCAG TACCAGCTCA 
GCTGGTAGCA ACAACAGTGC TGAGGGAGCT GGCCTGACCG ATAATGGATG TCGCCGGCGG 
CGCCAGGATC GGGCCAAGGA GAGGCCACGA CTGAAGAAGC AGGTATCTTT GTCCTCAACT 
GATTCCTTAG ATGAGGACAG AGTTGATGAG CCTGATGTCC TAGGGTCCAG GAGGAGCTCA 
GAAAGGAAGA AGCATTTAGA AAGAAATTGG TCTGGCTGTG GTGAGGGTGA ACAGAAAAGC 
AATGGTAAAG AAAACCGAAG TGCCCTTCGT GTCACTTTTG ATGCAGAAAC CATGAGTAAA 
GACTCCCCTG TGGTGAGGTC AGTCAAAGAT AATGTGGATA GAATGAAGTC TGACAAAGGC 
CCAAGCAGTG GGGGTAAGGG CTCTGAGAAG CAGGAGCTCA GACACCCGAG ACAAGAACTT 
CGAGATCGTG GTCGTGGTAT CCTGATTCTG CCTGCCCACA CTGCCCTATC TGTCAGTTCA 
TCAGGTTCTC CGGAGTCTAC ACCTTTGGGA CCTCGACTTT TATTTGGATC TGGTAGTAAG 
GGATCTCGGA GTTGGGGCCG TGGAGGCACT ACCCGCCGAC TATGGGACCC AAACAATCCT 
GACCAGAAGC CTGCCCTAAA AAGCCAGACA CCTCAGCTAC ATTTCTTGGA CACTGATGAT 
GAAATTAGCC CTACATCTTG GGGTGATTCA CGTCAGGCCC AAGCATCTTA CTATAAATTT 
CAAAACTCTG ATAACCCCTA TTATTACCCT CGGACACCAG GCCCTGCCTC TCAGTATCCC 
TATGCAGGCT ATAGCCCTCT GCAGTACCCA GTAGGCCCTA CAAATGGTAT GTATCCAGGG 
GCTTACTACC CGGGCTATCC TGCCCCCTCA GGACAATATG TGTGTAGCCC TCTCCCTGCT 
AGCACCATGA GTCCTGAGGA AATAGAACAG CACGTGCGGA ACATGCAGCA ACAGGAGCTC 
CATCGGCTTC TCCGAGTGGC TGACAACCAG GAACTGCAGC TCAGCAACCT GCTTTCCAGG 
GACCGCATCA GCACTGAGGG CATGGAGAAG ATGGCTCAGC TCAGAACTGA ACTACTGCAG 
CTGTACGAGC GCTGTATTCT ATTAGATATT GAATTCTCCG ACAGTCAGAA TGTAGATCAG 
ATCCTGTGGA AGAACGCTTT CTATCAGGTG ATTGAGAAGT TCAGGCAGCT TCTCAAGGAT 
CCAAACAGTG AGAACCCAGA GCAGATTCGG AACAGACTCT TGGAGCTCTT AGATGAGGGC 
AGTGACTTCT TTGATAGTTT GCTTCAGAAA CTACAGGTTA CTTACAAGTT CAAACTGGAG 
GACTACATGG ATGGGCTTGC CATTCGAAGC AAGCCTTTAC GAAAAACAGT GAAATATGCC 
TTGATCAGTG CTCAACGAAG TATGATTTGC CAAGGAGATA TCTCTAGGTA TCGGGAGCAA 
GCCAACGATA CAGCAAACTA TGGGAAGGCT CGCAGTTGGT ATCTGAAGGC TCAGCACATT 
GCTCCCAAGA ATGGGCGCCC CTATAACCAG TTGGCTCTAC TGGCAGTGTA TACGAGGAGG 
AAGCTTGATG CTGTATATTA CTATATGCGC AGTTTAGCAG CCAGCAACCC TATCCTGACT 
GCCAAGGAGA GTCTCATGAG TTTATTTGAA GAGACTAAAC GAAAGGCTGA ACAGATGGAA 
AAGAAGCAGC ATGAGGAATT TGATATGAGC CCTGATAAGT GGCGAAAAGG AAAGAAGTCT 
ACTTTCCGGC ATGTTGGGGA TGACACTACT CGCCTGGAGA TTTGGATTCA TCCATCACAT 
TCTCGGTCTG CCCAGGGCAC TGAGTCTGGG AAGGATTCTG AGCAGGAGAA TGGTCTGGGC 
AGCCTGAGTC CCAGTGATCT GAACAAAAGG TTCATCCTCA GTTTTCTCCA TGCCCATGGG 
AAGCTGTTTA CCCGGATTGG GATGGAGACA TTCCCTGCAG TGGCTGAGAA GGTCCTTAAG 
GAGTTCCAAG TATTGCTGCA GCATAGCCCA TCTCCTATTG GAAGTACCCG TATGCTGCAG 
CTTATGACCA TCAACATGTT TGCCGTGCAT AACTCCCAGT TGAAAGACTG CTTCTCAGAG 
GAGTGTCGTT CTGTGATCCA GGAACAAGCT GCATCCTTGG GCTTGGCTAT GTTTTCTCTA 
CTGGTCCAGC GCTGCACATG CCTACTTAAG GACTCTGCTA AAGCTCAGCT GTCCTCTCCT 
GAGGACCAAG AGGACCAAGA TGACATCAAG GTATCTTCCT TCGTCCCGGA CCTGAAGGAG 
CTGCTCCCCA GTGTGAAAGT CTGGTCTGAT TGGATGCTTG GCTACCCAGA CACCTGGAAT 
CCTCCACCCA CATCTTTGGA TCTGCCCTTG CAGGTTGCTG TGGATGTCTG GTCAACACTA 
GCTGATTTCT GTAACATACT GACTGCAGTC AATCAGTCTG AGGTGCCACT GTACAAGGAC 
CCAGATGATG ACCTCACCCT GCTTATCCTG GAAGAGGATC GGCTTCTCTC TGGCTTTGTC 
CCCTTGCTGG CTGCCCCTCA GGACCCCTGC TACGTGGAGA AAACCTCGGA TAAGGTTATT 
GCAGCTGACT GCAAAAGGGT CACAGTGCTG AAGTATTTTC TGGAAGCCCT TTGTGGACAA 
GAAGAGCCTC TGCTGGCATT CAAGGGTGGA AAATATGTGT CAGTGGCACC CGTCCCAGAC 
ACCATGGGAA AGGAAATGGG AAGCCAAGAG GGAAAACAAC TGGAAGATGA GGAAGAAGAT 
GTGGTGATTG AAGACTTTGA GGAAGATTCA GAGGCTGAAG GCAGTGGGGG TGAGGATGAC 
ATCAGGGAAC TTCGGGCCAA GAAGCTGGCT CTAGCAAGGA AGATAGCTGA GCAGCAGCGT 
CGCCAGGAAA AGATCCAGGC TGTTTTGGAA GACCAGAGTC AGATGAGGCA GATGGAGCTG 
GAGATCAGAC CCTTGTTCCT CGTACCAGAT ACCAACGGCT TCATTGACCA CCTGGCCAGT 
CTGGCTCGGC TGCTGGAGAG CAGGAAGTAC ATCCTGGTGG TGCCCCTCAT CGTGATCAAT 
GAGCTGGACG GCCTCGCCAA AGGGCAGGAG ACAGACCACC GGGCTGGGGG CTATGCCCGT 
GTGGTGCAAG AAAAGGCCCG AAAATCCATT GAGTTCCTCG AACGGAGATT TGAGAGTCGG 
GACTCCTGCC TGCGGGCCTT GACCAGCCGT GGCAATGAAC TTGAATCTAT CGCCTTCCGA 
AGTGAGGACA TTACTGGACA GCTGGGTAAC AACGATGACC TGATCCTCTC TTGCTGCCTG 
CACTACTGCA AAGACAAGGC TAAGGATTAC ATGCCCACCA GCAAAGAGGA GCCAATCCGC 
CTGTTACGGG AGGTGGTGTT GCTGACCGAT GATCGGAACC TTCGTGTAAA GGCGCTGACA 
AGGAATGTGC CTGTGAGGGA CATCCCAGCC TTCCTTACGT GGGCCCAGGT GGGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001002764.1
CDS	63..4319
Translation	MAEGLERVRISASELRGILATLAPQAGSRENMKELKEPRQRKDNRRPDLEIYKPGLSRLRNRPKTKEASGNEEFKDEIVNDRDSSAVGNDTQLIQVCKELDSQQQNGPIDAENSQAQETFPKTVGLEDRSLKIIKRSKKPDLQIYQPGRRLQTITKESAGRADEEEILNQVEQLRIEEDECKGEAIKEEVNNKPDKTEIEKHQSNDRVRTAKGEKGKKIEKGEGSKKVADDSVPGKPGSVKRYSRSDKRRNRYRTCSTSSAGSNNSAEGAGLTDNGCRRRRQDRAKERPRLKKQVSLSSTDSLDEDRVDEPDVLGSRRSSERKKHLERNWSGCGEGEQKSNGKENRSALRVTFDAETMSKDSPVVRSVKDNVDRMKSDKGPSSGGKGSEKQELRHPRQELRDRGRGILILPAHTALSVSSSGSPESTPLGPRLLFGSGSKGSRSWGRGGTTRRLWDPNNPDQKPALKSQTPQLHFLDTDDEISPTSWGDSRQAQASYYKFQNSDNPYYYPRTPGPASQYPYAGYSPLQYPVGPTNGMYPGAYYPGYPAPSGQYVCSPLPASTMSPEEIEQHVRNMQQQELHRLLRVADNQELQLSNLLSRDRISTEGMEKMAQLRTELLQLYERCILLDIEFSDSQNVDQILWKNAFYQVIEKFRQLLKDPNSENPEQIRNRLLELLDEGSDFFDSLLQKLQVTYKFKLEDYMDGLAIRSKPLRKTVKYALISAQRSMICQGDISRYREQANDTANYGKARSWYLKAQHIAPKNGRPYNQLALLAVYTRRKLDAVYYYMRSLAASNPILTAKESLMSLFEETKRKAEQMEKKQHEEFDMSPDKWRKGKKSTFRHVGDDTTRLEIWIHPSHSRSAQGTESGKDSEQENGLGSLSPSDLNKRFILSFLHAHGKLFTRIGMETFPAVAEKVLKEFQVLLQHSPSPIGSTRMLQLMTINMFAVHNSQLKDCFSEECRSVIQEQAASLGLAMFSLLVQRCTCLLKDSAKAQLSSPEDQEDQDDIKVSSFVPDLKELLPSVKVWSDWMLGYPDTWNPPPTSLDLPLQVAVDVWSTLADFCNILTAVNQSEVPLYKDPDDDLTLLILEEDRLLSGFVPLLAAPQDPCYVEKTSDKVIAADCKRVTVLKYFLEALCGQEEPLLAFKGGKYVSVAPVPDTMGKEMGSQEGKQLEDEEEDVVIEDFEEDSEAEGSGGEDDIRELRAKKLALARKIAEQQRRQEKIQAVLEDQSQMRQMELEIRPLFLVPDTNGFIDHLASLARLLESRKYILVVPLIVINELDGLAKGQETDHRAGGYARVVQEKARKSIEFLERRFESRDSCLRALTSRGNELESIAFRSEDITGQLGNNDDLILSCCLHYCKDKAKDYMPTSKEEPIRLLREVVLLTDDRNLRVKALTRNVPVRDIPAFLTWAQVG

Target ORF information:

RefSeq Version	NM_001002764.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus Smg-6 homolog, nonsense mediated mRNA decay factor (C. elegans) (Smg6), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001002764.2

ORF Insert Sequence:

ATGGCGGAGG GGTTGGAGCG TGTGCGGATC TCCGCATCGG AACTGCGAGG GATCCTGGCC 
ACTCTGGCTC CGCAGGCCGG GAGCAGAGAA AACATGAAGG AACTAAAGGA ACCCAGACAG 
CGCAAAGATA ATAGGCGTCC AGATTTGGAA ATCTATAAGC CTGGACTTTC TCGACTAAGG 
AACAGACCTA AAACTAAGGA GGCTTCTGGG AATGAGGAAT TTAAGGATGA GATAGTAAAT 
GACAGAGATT CTTCTGCTGT TGGGAATGAT ACACAGCTTA TTCAAGTTTG CAAGGAATTG 
GACAGCCAAC AGCAAAATGG CCCTATAGAT GCAGAAAATA GTCAGGCACA GGAAACTTTT 
CCAAAGACTG TTGGACTAGA GGACCGAAGT CTAAAGATCA TCAAAAGAAG CAAGAAACCA 
GACCTGCAGA TCTACCAGCC TGGAAGGCGG TTGCAGACCA TTACCAAAGA GTCAGCAGGC 
AGGGCAGATG AAGAAGAGAT CCTCAACCAA GTGGAGCAGT TGAGGATAGA GGAGGATGAG 
TGTAAGGGAG AAGCTATAAA AGAAGAAGTT AATAACAAAC CGGATAAAAC CGAGATAGAA 
AAGCACCAAA GTAATGACCG TGTAAGAACT GCCAAGGGAG AAAAGGGAAA AAAGATTGAA 
AAGGGGGAGG GGTCAAAGAA GGTGGCTGAT GACTCTGTCC CAGGAAAGCC TGGCTCTGTA 
AAGAGATACT CGAGATCAGA CAAGAGAAGG AATCGCTACC GCACTTGCAG TACCAGCTCA 
GCTGGTAGCA ACAACAGTGC TGAGGGAGCT GGCCTGACCG ATAATGGATG TCGCCGGCGG 
CGCCAGGATC GGGCCAAGGA GAGGCCACGA CTGAAGAAGC AGGTATCTTT GTCCTCAACT 
GATTCCTTAG ATGAGGACAG AGTTGATGAG CCTGATGTCC TAGGGTCCAG GAGGAGCTCA 
GAAAGGAAGA AGCATTTAGA AAGAAATTGG TCTGGCTGTG GTGAGGGTGA ACAGAAAAGC 
AATGGTAAAG AAAACCGAAG TGCCCTTCGT GTCACTTTTG ATGCAGAAAC CATGAGTAAA 
GACTCCCCTG TGGTGAGGTC AGTCAAAGAT AATGTGGATA GAATGAAGTC TGACAAAGGC 
CCAAGCAGTG GGGGTAAGGG CTCTGAGAAG CAGGAGCTCA GACACCCGAG ACAAGAACTT 
CGAGATCGTG GTCGTGGTAT CCTGATTCTG CCTGCCCACA CTGCCCTATC TGTCAGTTCA 
TCAGGTTCTC CGGAGTCTAC ACCTTTGGGA CCTCGACTTT TATTTGGATC TGGTAGTAAG 
GGATCTCGGA GTTGGGGCCG TGGAGGCACT ACCCGCCGAC TATGGGACCC AAACAATCCT 
GACCAGAAGC CTGCCCTAAA AAGCCAGACA CCTCAGCTAC ATTTCTTGGA CACTGATGAT 
GAAATTAGCC CTACATCTTG GGGTGATTCA CGTCAGGCCC AAGCATCTTA CTATAAATTT 
CAAAACTCTG ATAACCCCTA TTATTACCCT CGGACACCAG GCCCTGCCTC TCAGTATCCC 
TATGCAGGCT ATAGCCCTCT GCAGTACCCA GTAGGCCCTA CAAATGGTAT GTATCCAGGG 
GCTTACTACC CGGGCTATCC TGCCCCCTCA GGACAATATG TGTGTAGCCC TCTCCCTGCT 
AGCACCATGA GTCCTGAGGA AATAGAACAG CACGTGCGGA ACATGCAGCA ACAGGAGCTC 
CATCGGCTTC TCCGAGTGGC TGACAACCAG GAACTGCAGC TCAGCAACCT GCTTTCCAGG 
GACCGCATCA GCACTGAGGG CATGGAGAAG ATGGCTCAGC TCAGAACTGA ACTACTGCAG 
CTGTACGAGC GCTGTATTCT ATTAGATATT GAATTCTCCG ACAGTCAGAA TGTAGATCAG 
ATCCTGTGGA AGAACGCTTT CTATCAGGTG ATTGAGAAGT TCAGGCAGCT TCTCAAGGAT 
CCAAACAGTG AGAACCCAGA GCAGATTCGG AACAGACTCT TGGAGCTCTT AGATGAGGGC 
AGTGACTTCT TTGATAGTTT GCTTCAGAAA CTACAGGTTA CTTACAAGTT CAAACTGGAG 
GACTACATGG ATGGGCTTGC CATTCGAAGC AAGCCTTTAC GAAAAACAGT GAAATATGCC 
TTGATCAGTG CTCAACGAAG TATGATTTGC CAAGGAGATA TCTCTAGGTA TCGGGAGCAA 
GCCAACGATA CAGCAAACTA TGGGAAGGCT CGCAGTTGGT ATCTGAAGGC TCAGCACATT 
GCTCCCAAGA ATGGGCGCCC CTATAACCAG TTGGCTCTAC TGGCAGTGTA TACGAGGAGG 
AAGCTTGATG CTGTATATTA CTATATGCGC AGTTTAGCAG CCAGCAACCC TATCCTGACT 
GCCAAGGAGA GTCTCATGAG TTTATTTGAA GAGACTAAAC GAAAGGCTGA ACAGATGGAA 
AAGAAGCAGC ATGAGGAATT TGATATGAGC CCTGATAAGT GGCGAAAAGG AAAGAAGTCT 
ACTTTCCGGC ATGTTGGGGA TGACACTACT CGCCTGGAGA TTTGGATTCA TCCATCACAT 
TCTCGGTCTG CCCAGGGCAC TGAGTCTGGG AAGGATTCTG AGCAGGAGAA TGGTCTGGGC 
AGCCTGAGTC CCAGTGATCT GAACAAAAGG TTCATCCTCA GTTTTCTCCA TGCCCATGGG 
AAGCTGTTTA CCCGGATTGG GATGGAGACA TTCCCTGCAG TGGCTGAGAA GGTCCTTAAG 
GAGTTCCAAG TATTGCTGCA GCATAGCCCA TCTCCTATTG GAAGTACCCG TATGCTGCAG 
CTTATGACCA TCAACATGTT TGCCGTGCAT AACTCCCAGT TGAAAGACTG CTTCTCAGAG 
GAGTGTCGTT CTGTGATCCA GGAACAAGCT GCATCCTTGG GCTTGGCTAT GTTTTCTCTA 
CTGGTCCAGC GCTGCACATG CCTACTTAAG GACTCTGCTA AAGCTCAGCT GTCCTCTCCT 
GAGGACCAAG AGGACCAAGA TGACATCAAG GTATCTTCCT TCGTCCCGGA CCTGAAGGAG 
CTGCTCCCCA GTGTGAAAGT CTGGTCTGAT TGGATGCTTG GCTACCCAGA CACCTGGAAT 
CCTCCACCCA CATCTTTGGA TCTGCCCTTG CAGGTTGCTG TGGATGTCTG GTCAACACTA 
GCTGATTTCT GTAACATACT GACTGCAGTC AATCAGTCTG AGGTGCCACT GTACAAGGAC 
CCAGATGATG ACCTCACCCT GCTTATCCTG GAAGAGGATC GGCTTCTCTC TGGCTTTGTC 
CCCTTGCTGG CTGCCCCTCA GGACCCCTGC TACGTGGAGA AAACCTCGGA TAAGGTTATT 
GCAGCTGACT GCAAAAGGGT CACAGTGCTG AAGTATTTTC TGGAAGCCCT TTGTGGACAA 
GAAGAGCCTC TGCTGGCATT CAAGGGTGGA AAATATGTGT CAGTGGCACC CGTCCCAGAC 
ACCATGGGAA AGGAAATGGG AAGCCAAGAG GGAAAACAAC TGGAAGATGA GGAAGAAGAT 
GTGGTGATTG AAGACTTTGA GGAAGATTCA GAGGCTGAAG GCAGTGGGGG TGAGGATGAC 
ATCAGGGAAC TTCGGGCCAA GAAGCTGGCT CTAGCAAGGA AGATAGCTGA GCAGCAGCGT 
CGCCAGGAAA AGATCCAGGC TGTTTTGGAA GACCAGAGTC AGATGAGGCA GATGGAGCTG 
GAGATCAGAC CCTTGTTCCT CGTACCAGAT ACCAACGGCT TCATTGACCA CCTGGCCAGT 
CTGGCTCGGC TGCTGGAGAG CAGGAAGTAC ATCCTGGTGG TGCCCCTCAT CGTGATCAAT 
GAGCTGGACG GCCTCGCCAA AGGGCAGGAG ACAGACCACC GGGCTGGGGG CTATGCCCGT 
GTGGTGCAAG AAAAGGCCCG AAAATCCATT GAGTTCCTCG AACGGAGATT TGAGAGTCGG 
GACTCCTGCC TGCGGGCCTT GACCAGCCGT GGCAATGAAC TTGAATCTAT CGCCTTCCGA 
AGTGAGGACA TTACTGGACA GCTGGGTAAC AACGATGACC TGATCCTCTC TTGCTGCCTG 
CACTACTGCA AAGACAAGGC TAAGGATTAC ATGCCCACCA GCAAAGAGGA GCCAATCCGC 
CTGTTACGGG AGGTGGTGTT GCTGACCGAT GATCGGAACC TTCGTGTAAA GGCGCTGACA 
AGGAATGTGC CTGTGAGGGA CATCCCAGCC TTCCTTACGT GGGCCCAGGT GGGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Smg6 ( NM_001002764.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001002764.1 Mus musculus Smg-6 homolog, nonsense mediated mRNA decay factor (C. elegans) (Smg6), mRNA.

Price & Availability↑

CloneID	OMu14550
Clone ID Related Accession (Same CDS sequence)	NM_001002764.2 , NM_001002764.1
Accession Version	NM_001002764.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4257bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-12-26
Organism	Mus musculus(house mouse)
Product	telomerase-binding protein EST1A
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC066040.1. On Jul 26, 2005 this sequence version replaced NM_146022.1. ##Evidence-Data-START## Transcript exon combination :: BC066040.1, BC047279.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END##

ATGGCGGAGG GGTTGGAGCG TGTGCGGATC TCCGCATCGG AACTGCGAGG GATCCTGGCC 
ACTCTGGCTC CGCAGGCCGG GAGCAGAGAA AACATGAAGG AACTAAAGGA ACCCAGACAG 
CGCAAAGATA ATAGGCGTCC AGATTTGGAA ATCTATAAGC CTGGACTTTC TCGACTAAGG 
AACAGACCTA AAACTAAGGA GGCTTCTGGG AATGAGGAAT TTAAGGATGA GATAGTAAAT 
GACAGAGATT CTTCTGCTGT TGGGAATGAT ACACAGCTTA TTCAAGTTTG CAAGGAATTG 
GACAGCCAAC AGCAAAATGG CCCTATAGAT GCAGAAAATA GTCAGGCACA GGAAACTTTT 
CCAAAGACTG TTGGACTAGA GGACCGAAGT CTAAAGATCA TCAAAAGAAG CAAGAAACCA 
GACCTGCAGA TCTACCAGCC TGGAAGGCGG TTGCAGACCA TTACCAAAGA GTCAGCAGGC 
AGGGCAGATG AAGAAGAGAT CCTCAACCAA GTGGAGCAGT TGAGGATAGA GGAGGATGAG 
TGTAAGGGAG AAGCTATAAA AGAAGAAGTT AATAACAAAC CGGATAAAAC CGAGATAGAA 
AAGCACCAAA GTAATGACCG TGTAAGAACT GCCAAGGGAG AAAAGGGAAA AAAGATTGAA 
AAGGGGGAGG GGTCAAAGAA GGTGGCTGAT GACTCTGTCC CAGGAAAGCC TGGCTCTGTA 
AAGAGATACT CGAGATCAGA CAAGAGAAGG AATCGCTACC GCACTTGCAG TACCAGCTCA 
GCTGGTAGCA ACAACAGTGC TGAGGGAGCT GGCCTGACCG ATAATGGATG TCGCCGGCGG 
CGCCAGGATC GGGCCAAGGA GAGGCCACGA CTGAAGAAGC AGGTATCTTT GTCCTCAACT 
GATTCCTTAG ATGAGGACAG AGTTGATGAG CCTGATGTCC TAGGGTCCAG GAGGAGCTCA 
GAAAGGAAGA AGCATTTAGA AAGAAATTGG TCTGGCTGTG GTGAGGGTGA ACAGAAAAGC 
AATGGTAAAG AAAACCGAAG TGCCCTTCGT GTCACTTTTG ATGCAGAAAC CATGAGTAAA 
GACTCCCCTG TGGTGAGGTC AGTCAAAGAT AATGTGGATA GAATGAAGTC TGACAAAGGC 
CCAAGCAGTG GGGGTAAGGG CTCTGAGAAG CAGGAGCTCA GACACCCGAG ACAAGAACTT 
CGAGATCGTG GTCGTGGTAT CCTGATTCTG CCTGCCCACA CTGCCCTATC TGTCAGTTCA 
TCAGGTTCTC CGGAGTCTAC ACCTTTGGGA CCTCGACTTT TATTTGGATC TGGTAGTAAG 
GGATCTCGGA GTTGGGGCCG TGGAGGCACT ACCCGCCGAC TATGGGACCC AAACAATCCT 
GACCAGAAGC CTGCCCTAAA AAGCCAGACA CCTCAGCTAC ATTTCTTGGA CACTGATGAT 
GAAATTAGCC CTACATCTTG GGGTGATTCA CGTCAGGCCC AAGCATCTTA CTATAAATTT 
CAAAACTCTG ATAACCCCTA TTATTACCCT CGGACACCAG GCCCTGCCTC TCAGTATCCC 
TATGCAGGCT ATAGCCCTCT GCAGTACCCA GTAGGCCCTA CAAATGGTAT GTATCCAGGG 
GCTTACTACC CGGGCTATCC TGCCCCCTCA GGACAATATG TGTGTAGCCC TCTCCCTGCT 
AGCACCATGA GTCCTGAGGA AATAGAACAG CACGTGCGGA ACATGCAGCA ACAGGAGCTC 
CATCGGCTTC TCCGAGTGGC TGACAACCAG GAACTGCAGC TCAGCAACCT GCTTTCCAGG 
GACCGCATCA GCACTGAGGG CATGGAGAAG ATGGCTCAGC TCAGAACTGA ACTACTGCAG 
CTGTACGAGC GCTGTATTCT ATTAGATATT GAATTCTCCG ACAGTCAGAA TGTAGATCAG 
ATCCTGTGGA AGAACGCTTT CTATCAGGTG ATTGAGAAGT TCAGGCAGCT TCTCAAGGAT 
CCAAACAGTG AGAACCCAGA GCAGATTCGG AACAGACTCT TGGAGCTCTT AGATGAGGGC 
AGTGACTTCT TTGATAGTTT GCTTCAGAAA CTACAGGTTA CTTACAAGTT CAAACTGGAG 
GACTACATGG ATGGGCTTGC CATTCGAAGC AAGCCTTTAC GAAAAACAGT GAAATATGCC 
TTGATCAGTG CTCAACGAAG TATGATTTGC CAAGGAGATA TCTCTAGGTA TCGGGAGCAA 
GCCAACGATA CAGCAAACTA TGGGAAGGCT CGCAGTTGGT ATCTGAAGGC TCAGCACATT 
GCTCCCAAGA ATGGGCGCCC CTATAACCAG TTGGCTCTAC TGGCAGTGTA TACGAGGAGG 
AAGCTTGATG CTGTATATTA CTATATGCGC AGTTTAGCAG CCAGCAACCC TATCCTGACT 
GCCAAGGAGA GTCTCATGAG TTTATTTGAA GAGACTAAAC GAAAGGCTGA ACAGATGGAA 
AAGAAGCAGC ATGAGGAATT TGATATGAGC CCTGATAAGT GGCGAAAAGG AAAGAAGTCT 
ACTTTCCGGC ATGTTGGGGA TGACACTACT CGCCTGGAGA TTTGGATTCA TCCATCACAT 
TCTCGGTCTG CCCAGGGCAC TGAGTCTGGG AAGGATTCTG AGCAGGAGAA TGGTCTGGGC 
AGCCTGAGTC CCAGTGATCT GAACAAAAGG TTCATCCTCA GTTTTCTCCA TGCCCATGGG 
AAGCTGTTTA CCCGGATTGG GATGGAGACA TTCCCTGCAG TGGCTGAGAA GGTCCTTAAG 
GAGTTCCAAG TATTGCTGCA GCATAGCCCA TCTCCTATTG GAAGTACCCG TATGCTGCAG 
CTTATGACCA TCAACATGTT TGCCGTGCAT AACTCCCAGT TGAAAGACTG CTTCTCAGAG 
GAGTGTCGTT CTGTGATCCA GGAACAAGCT GCATCCTTGG GCTTGGCTAT GTTTTCTCTA 
CTGGTCCAGC GCTGCACATG CCTACTTAAG GACTCTGCTA AAGCTCAGCT GTCCTCTCCT 
GAGGACCAAG AGGACCAAGA TGACATCAAG GTATCTTCCT TCGTCCCGGA CCTGAAGGAG 
CTGCTCCCCA GTGTGAAAGT CTGGTCTGAT TGGATGCTTG GCTACCCAGA CACCTGGAAT 
CCTCCACCCA CATCTTTGGA TCTGCCCTTG CAGGTTGCTG TGGATGTCTG GTCAACACTA 
GCTGATTTCT GTAACATACT GACTGCAGTC AATCAGTCTG AGGTGCCACT GTACAAGGAC 
CCAGATGATG ACCTCACCCT GCTTATCCTG GAAGAGGATC GGCTTCTCTC TGGCTTTGTC 
CCCTTGCTGG CTGCCCCTCA GGACCCCTGC TACGTGGAGA AAACCTCGGA TAAGGTTATT 
GCAGCTGACT GCAAAAGGGT CACAGTGCTG AAGTATTTTC TGGAAGCCCT TTGTGGACAA 
GAAGAGCCTC TGCTGGCATT CAAGGGTGGA AAATATGTGT CAGTGGCACC CGTCCCAGAC 
ACCATGGGAA AGGAAATGGG AAGCCAAGAG GGAAAACAAC TGGAAGATGA GGAAGAAGAT 
GTGGTGATTG AAGACTTTGA GGAAGATTCA GAGGCTGAAG GCAGTGGGGG TGAGGATGAC 
ATCAGGGAAC TTCGGGCCAA GAAGCTGGCT CTAGCAAGGA AGATAGCTGA GCAGCAGCGT 
CGCCAGGAAA AGATCCAGGC TGTTTTGGAA GACCAGAGTC AGATGAGGCA GATGGAGCTG 
GAGATCAGAC CCTTGTTCCT CGTACCAGAT ACCAACGGCT TCATTGACCA CCTGGCCAGT 
CTGGCTCGGC TGCTGGAGAG CAGGAAGTAC ATCCTGGTGG TGCCCCTCAT CGTGATCAAT 
GAGCTGGACG GCCTCGCCAA AGGGCAGGAG ACAGACCACC GGGCTGGGGG CTATGCCCGT 
GTGGTGCAAG AAAAGGCCCG AAAATCCATT GAGTTCCTCG AACGGAGATT TGAGAGTCGG 
GACTCCTGCC TGCGGGCCTT GACCAGCCGT GGCAATGAAC TTGAATCTAT CGCCTTCCGA 
AGTGAGGACA TTACTGGACA GCTGGGTAAC AACGATGACC TGATCCTCTC TTGCTGCCTG 
CACTACTGCA AAGACAAGGC TAAGGATTAC ATGCCCACCA GCAAAGAGGA GCCAATCCGC 
CTGTTACGGG AGGTGGTGTT GCTGACCGAT GATCGGAACC TTCGTGTAAA GGCGCTGACA 
AGGAATGTGC CTGTGAGGGA CATCCCAGCC TTCCTTACGT GGGCCCAGGT GGGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001002764.1
CDS	45..4301
Translation	MAEGLERVRISASELRGILATLAPQAGSRENMKELKEPRQRKDNRRPDLEIYKPGLSRLRNRPKTKEASGNEEFKDEIVNDRDSSAVGNDTQLIQVCKELDSQQQNGPIDAENSQAQETFPKTVGLEDRSLKIIKRSKKPDLQIYQPGRRLQTITKESAGRADEEEILNQVEQLRIEEDECKGEAIKEEVNNKPDKTEIEKHQSNDRVRTAKGEKGKKIEKGEGSKKVADDSVPGKPGSVKRYSRSDKRRNRYRTCSTSSAGSNNSAEGAGLTDNGCRRRRQDRAKERPRLKKQVSLSSTDSLDEDRVDEPDVLGSRRSSERKKHLERNWSGCGEGEQKSNGKENRSALRVTFDAETMSKDSPVVRSVKDNVDRMKSDKGPSSGGKGSEKQELRHPRQELRDRGRGILILPAHTALSVSSSGSPESTPLGPRLLFGSGSKGSRSWGRGGTTRRLWDPNNPDQKPALKSQTPQLHFLDTDDEISPTSWGDSRQAQASYYKFQNSDNPYYYPRTPGPASQYPYAGYSPLQYPVGPTNGMYPGAYYPGYPAPSGQYVCSPLPASTMSPEEIEQHVRNMQQQELHRLLRVADNQELQLSNLLSRDRISTEGMEKMAQLRTELLQLYERCILLDIEFSDSQNVDQILWKNAFYQVIEKFRQLLKDPNSENPEQIRNRLLELLDEGSDFFDSLLQKLQVTYKFKLEDYMDGLAIRSKPLRKTVKYALISAQRSMICQGDISRYREQANDTANYGKARSWYLKAQHIAPKNGRPYNQLALLAVYTRRKLDAVYYYMRSLAASNPILTAKESLMSLFEETKRKAEQMEKKQHEEFDMSPDKWRKGKKSTFRHVGDDTTRLEIWIHPSHSRSAQGTESGKDSEQENGLGSLSPSDLNKRFILSFLHAHGKLFTRIGMETFPAVAEKVLKEFQVLLQHSPSPIGSTRMLQLMTINMFAVHNSQLKDCFSEECRSVIQEQAASLGLAMFSLLVQRCTCLLKDSAKAQLSSPEDQEDQDDIKVSSFVPDLKELLPSVKVWSDWMLGYPDTWNPPPTSLDLPLQVAVDVWSTLADFCNILTAVNQSEVPLYKDPDDDLTLLILEEDRLLSGFVPLLAAPQDPCYVEKTSDKVIAADCKRVTVLKYFLEALCGQEEPLLAFKGGKYVSVAPVPDTMGKEMGSQEGKQLEDEEEDVVIEDFEEDSEAEGSGGEDDIRELRAKKLALARKIAEQQRRQEKIQAVLEDQSQMRQMELEIRPLFLVPDTNGFIDHLASLARLLESRKYILVVPLIVINELDGLAKGQETDHRAGGYARVVQEKARKSIEFLERRFESRDSCLRALTSRGNELESIAFRSEDITGQLGNNDDLILSCCLHYCKDKAKDYMPTSKEEPIRLLREVVLLTDDRNLRVKALTRNVPVRDIPAFLTWAQVG

Target ORF information:

RefSeq Version	NM_001002764.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus Smg-6 homolog, nonsense mediated mRNA decay factor (C. elegans) (Smg6), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001002764.1

ORF Insert Sequence:

ATGGCGGAGG GGTTGGAGCG TGTGCGGATC TCCGCATCGG AACTGCGAGG GATCCTGGCC 
ACTCTGGCTC CGCAGGCCGG GAGCAGAGAA AACATGAAGG AACTAAAGGA ACCCAGACAG 
CGCAAAGATA ATAGGCGTCC AGATTTGGAA ATCTATAAGC CTGGACTTTC TCGACTAAGG 
AACAGACCTA AAACTAAGGA GGCTTCTGGG AATGAGGAAT TTAAGGATGA GATAGTAAAT 
GACAGAGATT CTTCTGCTGT TGGGAATGAT ACACAGCTTA TTCAAGTTTG CAAGGAATTG 
GACAGCCAAC AGCAAAATGG CCCTATAGAT GCAGAAAATA GTCAGGCACA GGAAACTTTT 
CCAAAGACTG TTGGACTAGA GGACCGAAGT CTAAAGATCA TCAAAAGAAG CAAGAAACCA 
GACCTGCAGA TCTACCAGCC TGGAAGGCGG TTGCAGACCA TTACCAAAGA GTCAGCAGGC 
AGGGCAGATG AAGAAGAGAT CCTCAACCAA GTGGAGCAGT TGAGGATAGA GGAGGATGAG 
TGTAAGGGAG AAGCTATAAA AGAAGAAGTT AATAACAAAC CGGATAAAAC CGAGATAGAA 
AAGCACCAAA GTAATGACCG TGTAAGAACT GCCAAGGGAG AAAAGGGAAA AAAGATTGAA 
AAGGGGGAGG GGTCAAAGAA GGTGGCTGAT GACTCTGTCC CAGGAAAGCC TGGCTCTGTA 
AAGAGATACT CGAGATCAGA CAAGAGAAGG AATCGCTACC GCACTTGCAG TACCAGCTCA 
GCTGGTAGCA ACAACAGTGC TGAGGGAGCT GGCCTGACCG ATAATGGATG TCGCCGGCGG 
CGCCAGGATC GGGCCAAGGA GAGGCCACGA CTGAAGAAGC AGGTATCTTT GTCCTCAACT 
GATTCCTTAG ATGAGGACAG AGTTGATGAG CCTGATGTCC TAGGGTCCAG GAGGAGCTCA 
GAAAGGAAGA AGCATTTAGA AAGAAATTGG TCTGGCTGTG GTGAGGGTGA ACAGAAAAGC 
AATGGTAAAG AAAACCGAAG TGCCCTTCGT GTCACTTTTG ATGCAGAAAC CATGAGTAAA 
GACTCCCCTG TGGTGAGGTC AGTCAAAGAT AATGTGGATA GAATGAAGTC TGACAAAGGC 
CCAAGCAGTG GGGGTAAGGG CTCTGAGAAG CAGGAGCTCA GACACCCGAG ACAAGAACTT 
CGAGATCGTG GTCGTGGTAT CCTGATTCTG CCTGCCCACA CTGCCCTATC TGTCAGTTCA 
TCAGGTTCTC CGGAGTCTAC ACCTTTGGGA CCTCGACTTT TATTTGGATC TGGTAGTAAG 
GGATCTCGGA GTTGGGGCCG TGGAGGCACT ACCCGCCGAC TATGGGACCC AAACAATCCT 
GACCAGAAGC CTGCCCTAAA AAGCCAGACA CCTCAGCTAC ATTTCTTGGA CACTGATGAT 
GAAATTAGCC CTACATCTTG GGGTGATTCA CGTCAGGCCC AAGCATCTTA CTATAAATTT 
CAAAACTCTG ATAACCCCTA TTATTACCCT CGGACACCAG GCCCTGCCTC TCAGTATCCC 
TATGCAGGCT ATAGCCCTCT GCAGTACCCA GTAGGCCCTA CAAATGGTAT GTATCCAGGG 
GCTTACTACC CGGGCTATCC TGCCCCCTCA GGACAATATG TGTGTAGCCC TCTCCCTGCT 
AGCACCATGA GTCCTGAGGA AATAGAACAG CACGTGCGGA ACATGCAGCA ACAGGAGCTC 
CATCGGCTTC TCCGAGTGGC TGACAACCAG GAACTGCAGC TCAGCAACCT GCTTTCCAGG 
GACCGCATCA GCACTGAGGG CATGGAGAAG ATGGCTCAGC TCAGAACTGA ACTACTGCAG 
CTGTACGAGC GCTGTATTCT ATTAGATATT GAATTCTCCG ACAGTCAGAA TGTAGATCAG 
ATCCTGTGGA AGAACGCTTT CTATCAGGTG ATTGAGAAGT TCAGGCAGCT TCTCAAGGAT 
CCAAACAGTG AGAACCCAGA GCAGATTCGG AACAGACTCT TGGAGCTCTT AGATGAGGGC 
AGTGACTTCT TTGATAGTTT GCTTCAGAAA CTACAGGTTA CTTACAAGTT CAAACTGGAG 
GACTACATGG ATGGGCTTGC CATTCGAAGC AAGCCTTTAC GAAAAACAGT GAAATATGCC 
TTGATCAGTG CTCAACGAAG TATGATTTGC CAAGGAGATA TCTCTAGGTA TCGGGAGCAA 
GCCAACGATA CAGCAAACTA TGGGAAGGCT CGCAGTTGGT ATCTGAAGGC TCAGCACATT 
GCTCCCAAGA ATGGGCGCCC CTATAACCAG TTGGCTCTAC TGGCAGTGTA TACGAGGAGG 
AAGCTTGATG CTGTATATTA CTATATGCGC AGTTTAGCAG CCAGCAACCC TATCCTGACT 
GCCAAGGAGA GTCTCATGAG TTTATTTGAA GAGACTAAAC GAAAGGCTGA ACAGATGGAA 
AAGAAGCAGC ATGAGGAATT TGATATGAGC CCTGATAAGT GGCGAAAAGG AAAGAAGTCT 
ACTTTCCGGC ATGTTGGGGA TGACACTACT CGCCTGGAGA TTTGGATTCA TCCATCACAT 
TCTCGGTCTG CCCAGGGCAC TGAGTCTGGG AAGGATTCTG AGCAGGAGAA TGGTCTGGGC 
AGCCTGAGTC CCAGTGATCT GAACAAAAGG TTCATCCTCA GTTTTCTCCA TGCCCATGGG 
AAGCTGTTTA CCCGGATTGG GATGGAGACA TTCCCTGCAG TGGCTGAGAA GGTCCTTAAG 
GAGTTCCAAG TATTGCTGCA GCATAGCCCA TCTCCTATTG GAAGTACCCG TATGCTGCAG 
CTTATGACCA TCAACATGTT TGCCGTGCAT AACTCCCAGT TGAAAGACTG CTTCTCAGAG 
GAGTGTCGTT CTGTGATCCA GGAACAAGCT GCATCCTTGG GCTTGGCTAT GTTTTCTCTA 
CTGGTCCAGC GCTGCACATG CCTACTTAAG GACTCTGCTA AAGCTCAGCT GTCCTCTCCT 
GAGGACCAAG AGGACCAAGA TGACATCAAG GTATCTTCCT TCGTCCCGGA CCTGAAGGAG 
CTGCTCCCCA GTGTGAAAGT CTGGTCTGAT TGGATGCTTG GCTACCCAGA CACCTGGAAT 
CCTCCACCCA CATCTTTGGA TCTGCCCTTG CAGGTTGCTG TGGATGTCTG GTCAACACTA 
GCTGATTTCT GTAACATACT GACTGCAGTC AATCAGTCTG AGGTGCCACT GTACAAGGAC 
CCAGATGATG ACCTCACCCT GCTTATCCTG GAAGAGGATC GGCTTCTCTC TGGCTTTGTC 
CCCTTGCTGG CTGCCCCTCA GGACCCCTGC TACGTGGAGA AAACCTCGGA TAAGGTTATT 
GCAGCTGACT GCAAAAGGGT CACAGTGCTG AAGTATTTTC TGGAAGCCCT TTGTGGACAA 
GAAGAGCCTC TGCTGGCATT CAAGGGTGGA AAATATGTGT CAGTGGCACC CGTCCCAGAC 
ACCATGGGAA AGGAAATGGG AAGCCAAGAG GGAAAACAAC TGGAAGATGA GGAAGAAGAT 
GTGGTGATTG AAGACTTTGA GGAAGATTCA GAGGCTGAAG GCAGTGGGGG TGAGGATGAC 
ATCAGGGAAC TTCGGGCCAA GAAGCTGGCT CTAGCAAGGA AGATAGCTGA GCAGCAGCGT 
CGCCAGGAAA AGATCCAGGC TGTTTTGGAA GACCAGAGTC AGATGAGGCA GATGGAGCTG 
GAGATCAGAC CCTTGTTCCT CGTACCAGAT ACCAACGGCT TCATTGACCA CCTGGCCAGT 
CTGGCTCGGC TGCTGGAGAG CAGGAAGTAC ATCCTGGTGG TGCCCCTCAT CGTGATCAAT 
GAGCTGGACG GCCTCGCCAA AGGGCAGGAG ACAGACCACC GGGCTGGGGG CTATGCCCGT 
GTGGTGCAAG AAAAGGCCCG AAAATCCATT GAGTTCCTCG AACGGAGATT TGAGAGTCGG 
GACTCCTGCC TGCGGGCCTT GACCAGCCGT GGCAATGAAC TTGAATCTAT CGCCTTCCGA 
AGTGAGGACA TTACTGGACA GCTGGGTAAC AACGATGACC TGATCCTCTC TTGCTGCCTG 
CACTACTGCA AAGACAAGGC TAAGGATTAC ATGCCCACCA GCAAAGAGGA GCCAATCCGC 
CTGTTACGGG AGGTGGTGTT GCTGACCGAT GATCGGAACC TTCGTGTAAA GGCGCTGACA 
AGGAATGTGC CTGTGAGGGA CATCCCAGCC TTCCTTACGT GGGCCCAGGT GGGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Smg6 ( XM_011248659.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011246961.1 Mus musculus Smg-6 homolog, nonsense mediated mRNA decay factor (C. elegans) (Smg6), transcript variant X1, mRNA.

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CloneID	OMu72062
Clone ID Related Accession (Same CDS sequence)	XM_011248659.3 , XM_011248659.2
Accession Version	XM_011248659.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4218bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	telomerase-binding protein EST1A isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000077.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_011248659.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACTTGGA TACAAGAGAA TACAACTCCC CTCTATTTCC ATAACTCAAA AAACATGAAG 
GAACTAAAGG AACCCAGACA GCGCAAAGAT AATAGGCGTC CAGATTTGGA AATCTATAAG 
CCTGGACTTT CTCGACTAAG GAACAGACCT AAAACTAAGG AGGCTTCTGG GAATGAGGAA 
TTTAAGGATG AGATAGTAAA TGACAGAGAT TCTTCTGCTG TTGGGAATGA TACACAGCTT 
ATTCAAGTTT GCAAGGAATT GGACAGCCAA CAGCAAAATG GCCCTATAGA TGCAGAAAAT 
AGTCAGGCAC AGGAAACTTT TCCAAAGACT GTTGGACTAG AGGACCGAAG TCTAAAGATC 
ATCAAAAGAA GCAAGAAACC AGACCTGCAG ATCTACCAGC CTGGAAGGCG GTTGCAGACC 
ATTACCAAAG AGTCAGCAGG CAGGGCAGAT GAAGAAGAGA TCCTCAACCA AGTGGAGCAG 
TTGAGGATAG AGGAGGATGA GTGTAAGGGA GAAGCTATAA AAGAAGAAGT TAATAACAAA 
CCGGATAAAA CCGAGATAGA AAAGCACCAA AGTAATGACC GTGTAAGAAC TGCCAAGGGA 
GAAAAGGGAA AAAAGATTGA AAAGGGGGAG GGGTCAAAGA AGGTGGCTGA TGACTCTGTC 
CCAGGAAAGC CTGGCTCTGT AAAGAGATAC TCGAGATCAG ACAAGAGAAG GAATCGCTAC 
CGCACTTGCA GTACCAGCTC AGCTGGTAGC AACAACAGTG CTGAGGGAGC TGGCCTGACC 
GATAATGGAT GTCGCCGGCG GCGCCAGGAT CGGGCCAAGG AGAGGCCACG ACTGAAGAAG 
CAGGTATCTT TGTCCTCAAC TGATTCCTTA GATGAGGACA GAGTTGATGA GCCTGATGTC 
CTAGGGTCCA GGAGGAGCTC AGAAAGGAAG AAGCATTTAG AAAGAAATTG GTCTGGCTGT 
GGTGAGGGTG AACAGAAAAG CAATGGTAAA GAAAACCGAA GTGCCCTTCG TGTCACTTTT 
GATGCAGAAA CCATGAGTAA AGACTCCCCT GTGGTGAGGT CAGTCAAAGA TAATGTGGAT 
AGAATGAAGT CTGACAAAGG CCCAAGCAGT GGGGGTAAGG GCTCTGAGAA GCAGGAGCTC 
AGACACCCGA GACAAGAACT TCGAGATCGT GGTCGTGGTA TCCTGATTCT GCCTGCCCAC 
ACTGCCCTAT CTGTCAGTTC ATCAGGTTCT CCGGAGTCTA CACCTTTGGG ACCTCGACTT 
TTATTTGGAT CTGGTAGTAA GGGATCTCGG AGTTGGGGCC GTGGAGGCAC TACCCGCCGA 
CTATGGGACC CAAACAATCC TGACCAGAAG CCTGCCCTAA AAAGCCAGAC ACCTCAGCTA 
CATTTCTTGG ACACTGATGA TGAAATTAGC CCTACATCTT GGGGTGATTC ACGTCAGGCC 
CAAGCATCTT ACTATAAATT TCAAAACTCT GATAACCCCT ATTATTACCC TCGGACACCA 
GGCCCTGCCT CTCAGTATCC CTATGCAGGC TATAGCCCTC TGCAGTACCC AGTAGGCCCT 
ACAAATGGTA TGTATCCAGG GGCTTACTAC CCGGGCTATC CTGCCCCCTC AGGACAATAT 
GTGTGTAGCC CTCTCCCTGC TAGCACCATG AGTCCTGAGG AAATAGAACA GCACGTGCGG 
AACATGCAGC AACAGGAGCT CCATCGGCTT CTCCGAGTGG CTGACAACCA GGAACTGCAG 
CTCAGCAACC TGCTTTCCAG GGACCGCATC AGCACTGAGG GCATGGAGAA GATGGCTCAG 
CTCAGAACTG AACTACTGCA GCTGTACGAG CGCTGTATTC TATTAGATAT TGAATTCTCC 
GACAGTCAGA ATGTAGATCA GATCCTGTGG AAGAACGCTT TCTATCAGGT GATTGAGAAG 
TTCAGGCAGC TTCTCAAGGA TCCAAACAGT GAGAACCCAG AGCAGATTCG GAACAGACTC 
TTGGAGCTCT TAGATGAGGG CAGTGACTTC TTTGATAGTT TGCTTCAGAA ACTACAGGTT 
ACTTACAAGT TCAAACTGGA GGACTACATG GATGGGCTTG CCATTCGAAG CAAGCCTTTA 
CGAAAAACAG TGAAATATGC CTTGATCAGT GCTCAACGAA GTATGATTTG CCAAGGAGAT 
ATCTCTAGGT ATCGGGAGCA AGCCAACGAT ACAGCAAACT ATGGGAAGGC TCGCAGTTGG 
TATCTGAAGG CTCAGCACAT TGCTCCCAAG AATGGGCGCC CCTATAACCA GTTGGCTCTA 
CTGGCAGTGT ATACGAGGAG GAAGCTTGAT GCTGTATATT ACTATATGCG CAGTTTAGCA 
GCCAGCAACC CTATCCTGAC TGCCAAGGAG AGTCTCATGA GTTTATTTGA AGAGACTAAA 
CGAAAGGCTG AACAGATGGA AAAGAAGCAG CATGAGGAAT TTGATATGAG CCCTGATAAG 
TGGCGAAAAG GAAAGAAGTC TACTTTCCGG CATGTTGGGG ATGACACTAC TCGCCTGGAG 
ATTTGGATTC ATCCATCACA TTCTCGGTCT GCCCAGGGCA CTGAGTCTGG GAAGGATTCT 
GAGCAGGAGA ATGGTCTGGG CAGCCTGAGT CCCAGTGATC TGAACAAAAG GTTCATCCTC 
AGTTTTCTCC ATGCCCATGG GAAGCTGTTT ACCCGGATTG GGATGGAGAC ATTCCCTGCA 
GTGGCTGAGA AGGTCCTTAA GGAGTTCCAA GTATTGCTGC AGCATAGCCC ATCTCCTATT 
GGAAGTACCC GTATGCTGCA GCTTATGACC ATCAACATGT TTGCCGTGCA TAACTCCCAG 
TTGAAAGACT GCTTCTCAGA GGAGTGTCGT TCTGTGATCC AGGAACAAGC TGCATCCTTG 
GGCTTGGCTA TGTTTTCTCT ACTGGTCCAG CGCTGCACAT GCCTACTTAA GGACTCTGCT 
AAAGCTCAGC TGTCCTCTCC TGAGGACCAA GAGGACCAAG ATGACATCAA GGTATCTTCC 
TTCGTCCCGG ACCTGAAGGA GCTGCTCCCC AGTGTGAAAG TCTGGTCTGA TTGGATGCTT 
GGCTACCCAG ACACCTGGAA TCCTCCACCC ACATCTTTGG ATCTGCCCTT GCAGGTTGCT 
GTGGATGTCT GGTCAACACT AGCTGATTTC TGTAACATAC TGACTGCAGT CAATCAGTCT 
GAGGTGCCAC TGTACAAGGA CCCAGATGAT GACCTCACCC TGCTTATCCT GGAAGAGGAT 
CGGCTTCTCT CTGGCTTTGT CCCCTTGCTG GCTGCCCCTC AGGACCCCTG CTACGTGGAG 
AAAACCTCGG ATAAGGTTAT TGCAGCTGAC TGCAAAAGGG TCACAGTGCT GAAGTATTTT 
CTGGAAGCCC TTTGTGGACA AGAAGAGCCT CTGCTGGCAT TCAAGGGTGG AAAATATGTG 
TCAGTGGCAC CCGTCCCAGA CACCATGGGA AAGGAAATGG GAAGCCAAGA GGGAAAACAA 
CTGGAAGATG AGGAAGAAGA TGTGGTGATT GAAGACTTTG AGGAAGATTC AGAGGCTGAA 
GGCAGTGGGG GTGAGGATGA CATCAGGGAA CTTCGGGCCA AGAAGCTGGC TCTAGCAAGG 
AAGATAGCTG AGCAGCAGCG TCGCCAGGAA AAGATCCAGG CTGTTTTGGA AGACCAGAGT 
CAGATGAGGC AGATGGAGCT GGAGATCAGA CCCTTGTTCC TCGTACCAGA TACCAACGGC 
TTCATTGACC ACCTGGCCAG TCTGGCTCGG CTGCTGGAGA GCAGGAAGTA CATCCTGGTG 
GTGCCCCTCA TCGTGATCAA TGAGCTGGAC GGCCTCGCCA AAGGGCAGGA GACAGACCAC 
CGGGCTGGGG GCTATGCCCG TGTGGTGCAA GAAAAGGCCC GAAAATCCAT TGAGTTCCTC 
GAACGGAGAT TTGAGAGTCG GGACTCCTGC CTGCGGGCCT TGACCAGCCG TGGCAATGAA 
CTTGAATCTA TCGCCTTCCG AAGTGAGGAC ATTACTGGAC AGCTGGGTAA CAACGATGAC 
CTGATCCTCT CTTGCTGCCT GCACTACTGC AAAGACAAGG CTAAGGATTA CATGCCCACC 
AGCAAAGAGG AGCCAATCCG CCTGTTACGG GAGGTGGTGT TGCTGACCGA TGATCGGAAC 
CTTCGTGTAA AGGCGCTGAC AAGGAATGTG CCTGTGAGGG ACATCCCAGC CTTCCTTACG 
TGGGCCCAGG TGGGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011246961.1
CDS	383..4600
Translation	MTWIQENTTPLYFHNSKNMKELKEPRQRKDNRRPDLEIYKPGLSRLRNRPKTKEASGNEEFKDEIVNDRDSSAVGNDTQLIQVCKELDSQQQNGPIDAENSQAQETFPKTVGLEDRSLKIIKRSKKPDLQIYQPGRRLQTITKESAGRADEEEILNQVEQLRIEEDECKGEAIKEEVNNKPDKTEIEKHQSNDRVRTAKGEKGKKIEKGEGSKKVADDSVPGKPGSVKRYSRSDKRRNRYRTCSTSSAGSNNSAEGAGLTDNGCRRRRQDRAKERPRLKKQVSLSSTDSLDEDRVDEPDVLGSRRSSERKKHLERNWSGCGEGEQKSNGKENRSALRVTFDAETMSKDSPVVRSVKDNVDRMKSDKGPSSGGKGSEKQELRHPRQELRDRGRGILILPAHTALSVSSSGSPESTPLGPRLLFGSGSKGSRSWGRGGTTRRLWDPNNPDQKPALKSQTPQLHFLDTDDEISPTSWGDSRQAQASYYKFQNSDNPYYYPRTPGPASQYPYAGYSPLQYPVGPTNGMYPGAYYPGYPAPSGQYVCSPLPASTMSPEEIEQHVRNMQQQELHRLLRVADNQELQLSNLLSRDRISTEGMEKMAQLRTELLQLYERCILLDIEFSDSQNVDQILWKNAFYQVIEKFRQLLKDPNSENPEQIRNRLLELLDEGSDFFDSLLQKLQVTYKFKLEDYMDGLAIRSKPLRKTVKYALISAQRSMICQGDISRYREQANDTANYGKARSWYLKAQHIAPKNGRPYNQLALLAVYTRRKLDAVYYYMRSLAASNPILTAKESLMSLFEETKRKAEQMEKKQHEEFDMSPDKWRKGKKSTFRHVGDDTTRLEIWIHPSHSRSAQGTESGKDSEQENGLGSLSPSDLNKRFILSFLHAHGKLFTRIGMETFPAVAEKVLKEFQVLLQHSPSPIGSTRMLQLMTINMFAVHNSQLKDCFSEECRSVIQEQAASLGLAMFSLLVQRCTCLLKDSAKAQLSSPEDQEDQDDIKVSSFVPDLKELLPSVKVWSDWMLGYPDTWNPPPTSLDLPLQVAVDVWSTLADFCNILTAVNQSEVPLYKDPDDDLTLLILEEDRLLSGFVPLLAAPQDPCYVEKTSDKVIAADCKRVTVLKYFLEALCGQEEPLLAFKGGKYVSVAPVPDTMGKEMGSQEGKQLEDEEEDVVIEDFEEDSEAEGSGGEDDIRELRAKKLALARKIAEQQRRQEKIQAVLEDQSQMRQMELEIRPLFLVPDTNGFIDHLASLARLLESRKYILVVPLIVINELDGLAKGQETDHRAGGYARVVQEKARKSIEFLERRFESRDSCLRALTSRGNELESIAFRSEDITGQLGNNDDLILSCCLHYCKDKAKDYMPTSKEEPIRLLREVVLLTDDRNLRVKALTRNVPVRDIPAFLTWAQVG

Target ORF information:

RefSeq Version	XM_011248659.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus Smg-6 homolog, nonsense mediated mRNA decay factor (C. elegans) (Smg6), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011248659.3

ORF Insert Sequence:

ATGACTTGGA TACAAGAGAA TACAACTCCC CTCTATTTCC ATAACTCAAA AAACATGAAG 
GAACTAAAGG AACCCAGACA GCGCAAAGAT AATAGGCGTC CAGATTTGGA AATCTATAAG 
CCTGGACTTT CTCGACTAAG GAACAGACCT AAAACTAAGG AGGCTTCTGG GAATGAGGAA 
TTTAAGGATG AGATAGTAAA TGACAGAGAT TCTTCTGCTG TTGGGAATGA TACACAGCTT 
ATTCAAGTTT GCAAGGAATT GGACAGCCAA CAGCAAAATG GCCCTATAGA TGCAGAAAAT 
AGTCAGGCAC AGGAAACTTT TCCAAAGACT GTTGGACTAG AGGACCGAAG TCTAAAGATC 
ATCAAAAGAA GCAAGAAACC AGACCTGCAG ATCTACCAGC CTGGAAGGCG GTTGCAGACC 
ATTACCAAAG AGTCAGCAGG CAGGGCAGAT GAAGAAGAGA TCCTCAACCA AGTGGAGCAG 
TTGAGGATAG AGGAGGATGA GTGTAAGGGA GAAGCTATAA AAGAAGAAGT TAATAACAAA 
CCGGATAAAA CCGAGATAGA AAAGCACCAA AGTAATGACC GTGTAAGAAC TGCCAAGGGA 
GAAAAGGGAA AAAAGATTGA AAAGGGGGAG GGGTCAAAGA AGGTGGCTGA TGACTCTGTC 
CCAGGAAAGC CTGGCTCTGT AAAGAGATAC TCGAGATCAG ACAAGAGAAG GAATCGCTAC 
CGCACTTGCA GTACCAGCTC AGCTGGTAGC AACAACAGTG CTGAGGGAGC TGGCCTGACC 
GATAATGGAT GTCGCCGGCG GCGCCAGGAT CGGGCCAAGG AGAGGCCACG ACTGAAGAAG 
CAGGTATCTT TGTCCTCAAC TGATTCCTTA GATGAGGACA GAGTTGATGA GCCTGATGTC 
CTAGGGTCCA GGAGGAGCTC AGAAAGGAAG AAGCATTTAG AAAGAAATTG GTCTGGCTGT 
GGTGAGGGTG AACAGAAAAG CAATGGTAAA GAAAACCGAA GTGCCCTTCG TGTCACTTTT 
GATGCAGAAA CCATGAGTAA AGACTCCCCT GTGGTGAGGT CAGTCAAAGA TAATGTGGAT 
AGAATGAAGT CTGACAAAGG CCCAAGCAGT GGGGGTAAGG GCTCTGAGAA GCAGGAGCTC 
AGACACCCGA GACAAGAACT TCGAGATCGT GGTCGTGGTA TCCTGATTCT GCCTGCCCAC 
ACTGCCCTAT CTGTCAGTTC ATCAGGTTCT CCGGAGTCTA CACCTTTGGG ACCTCGACTT 
TTATTTGGAT CTGGTAGTAA GGGATCTCGG AGTTGGGGCC GTGGAGGCAC TACCCGCCGA 
CTATGGGACC CAAACAATCC TGACCAGAAG CCTGCCCTAA AAAGCCAGAC ACCTCAGCTA 
CATTTCTTGG ACACTGATGA TGAAATTAGC CCTACATCTT GGGGTGATTC ACGTCAGGCC 
CAAGCATCTT ACTATAAATT TCAAAACTCT GATAACCCCT ATTATTACCC TCGGACACCA 
GGCCCTGCCT CTCAGTATCC CTATGCAGGC TATAGCCCTC TGCAGTACCC AGTAGGCCCT 
ACAAATGGTA TGTATCCAGG GGCTTACTAC CCGGGCTATC CTGCCCCCTC AGGACAATAT 
GTGTGTAGCC CTCTCCCTGC TAGCACCATG AGTCCTGAGG AAATAGAACA GCACGTGCGG 
AACATGCAGC AACAGGAGCT CCATCGGCTT CTCCGAGTGG CTGACAACCA GGAACTGCAG 
CTCAGCAACC TGCTTTCCAG GGACCGCATC AGCACTGAGG GCATGGAGAA GATGGCTCAG 
CTCAGAACTG AACTACTGCA GCTGTACGAG CGCTGTATTC TATTAGATAT TGAATTCTCC 
GACAGTCAGA ATGTAGATCA GATCCTGTGG AAGAACGCTT TCTATCAGGT GATTGAGAAG 
TTCAGGCAGC TTCTCAAGGA TCCAAACAGT GAGAACCCAG AGCAGATTCG GAACAGACTC 
TTGGAGCTCT TAGATGAGGG CAGTGACTTC TTTGATAGTT TGCTTCAGAA ACTACAGGTT 
ACTTACAAGT TCAAACTGGA GGACTACATG GATGGGCTTG CCATTCGAAG CAAGCCTTTA 
CGAAAAACAG TGAAATATGC CTTGATCAGT GCTCAACGAA GTATGATTTG CCAAGGAGAT 
ATCTCTAGGT ATCGGGAGCA AGCCAACGAT ACAGCAAACT ATGGGAAGGC TCGCAGTTGG 
TATCTGAAGG CTCAGCACAT TGCTCCCAAG AATGGGCGCC CCTATAACCA GTTGGCTCTA 
CTGGCAGTGT ATACGAGGAG GAAGCTTGAT GCTGTATATT ACTATATGCG CAGTTTAGCA 
GCCAGCAACC CTATCCTGAC TGCCAAGGAG AGTCTCATGA GTTTATTTGA AGAGACTAAA 
CGAAAGGCTG AACAGATGGA AAAGAAGCAG CATGAGGAAT TTGATATGAG CCCTGATAAG 
TGGCGAAAAG GAAAGAAGTC TACTTTCCGG CATGTTGGGG ATGACACTAC TCGCCTGGAG 
ATTTGGATTC ATCCATCACA TTCTCGGTCT GCCCAGGGCA CTGAGTCTGG GAAGGATTCT 
GAGCAGGAGA ATGGTCTGGG CAGCCTGAGT CCCAGTGATC TGAACAAAAG GTTCATCCTC 
AGTTTTCTCC ATGCCCATGG GAAGCTGTTT ACCCGGATTG GGATGGAGAC ATTCCCTGCA 
GTGGCTGAGA AGGTCCTTAA GGAGTTCCAA GTATTGCTGC AGCATAGCCC ATCTCCTATT 
GGAAGTACCC GTATGCTGCA GCTTATGACC ATCAACATGT TTGCCGTGCA TAACTCCCAG 
TTGAAAGACT GCTTCTCAGA GGAGTGTCGT TCTGTGATCC AGGAACAAGC TGCATCCTTG 
GGCTTGGCTA TGTTTTCTCT ACTGGTCCAG CGCTGCACAT GCCTACTTAA GGACTCTGCT 
AAAGCTCAGC TGTCCTCTCC TGAGGACCAA GAGGACCAAG ATGACATCAA GGTATCTTCC 
TTCGTCCCGG ACCTGAAGGA GCTGCTCCCC AGTGTGAAAG TCTGGTCTGA TTGGATGCTT 
GGCTACCCAG ACACCTGGAA TCCTCCACCC ACATCTTTGG ATCTGCCCTT GCAGGTTGCT 
GTGGATGTCT GGTCAACACT AGCTGATTTC TGTAACATAC TGACTGCAGT CAATCAGTCT 
GAGGTGCCAC TGTACAAGGA CCCAGATGAT GACCTCACCC TGCTTATCCT GGAAGAGGAT 
CGGCTTCTCT CTGGCTTTGT CCCCTTGCTG GCTGCCCCTC AGGACCCCTG CTACGTGGAG 
AAAACCTCGG ATAAGGTTAT TGCAGCTGAC TGCAAAAGGG TCACAGTGCT GAAGTATTTT 
CTGGAAGCCC TTTGTGGACA AGAAGAGCCT CTGCTGGCAT TCAAGGGTGG AAAATATGTG 
TCAGTGGCAC CCGTCCCAGA CACCATGGGA AAGGAAATGG GAAGCCAAGA GGGAAAACAA 
CTGGAAGATG AGGAAGAAGA TGTGGTGATT GAAGACTTTG AGGAAGATTC AGAGGCTGAA 
GGCAGTGGGG GTGAGGATGA CATCAGGGAA CTTCGGGCCA AGAAGCTGGC TCTAGCAAGG 
AAGATAGCTG AGCAGCAGCG TCGCCAGGAA AAGATCCAGG CTGTTTTGGA AGACCAGAGT 
CAGATGAGGC AGATGGAGCT GGAGATCAGA CCCTTGTTCC TCGTACCAGA TACCAACGGC 
TTCATTGACC ACCTGGCCAG TCTGGCTCGG CTGCTGGAGA GCAGGAAGTA CATCCTGGTG 
GTGCCCCTCA TCGTGATCAA TGAGCTGGAC GGCCTCGCCA AAGGGCAGGA GACAGACCAC 
CGGGCTGGGG GCTATGCCCG TGTGGTGCAA GAAAAGGCCC GAAAATCCAT TGAGTTCCTC 
GAACGGAGAT TTGAGAGTCG GGACTCCTGC CTGCGGGCCT TGACCAGCCG TGGCAATGAA 
CTTGAATCTA TCGCCTTCCG AAGTGAGGAC ATTACTGGAC AGCTGGGTAA CAACGATGAC 
CTGATCCTCT CTTGCTGCCT GCACTACTGC AAAGACAAGG CTAAGGATTA CATGCCCACC 
AGCAAAGAGG AGCCAATCCG CCTGTTACGG GAGGTGGTGT TGCTGACCGA TGATCGGAAC 
CTTCGTGTAA AGGCGCTGAC AAGGAATGTG CCTGTGAGGG ACATCCCAGC CTTCCTTACG 
TGGGCCCAGG TGGGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Smg6 ( XM_011248659.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011246961.1 Mus musculus Smg-6 homolog, nonsense mediated mRNA decay factor (C. elegans) (Smg6), transcript variant X1, mRNA.

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CloneID	OMu72062
Clone ID Related Accession (Same CDS sequence)	XM_011248659.3 , XM_011248659.2
Accession Version	XM_011248659.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4218bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	telomerase-binding protein EST1A isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000077.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_011248659.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACTTGGA TACAAGAGAA TACAACTCCC CTCTATTTCC ATAACTCAAA AAACATGAAG 
GAACTAAAGG AACCCAGACA GCGCAAAGAT AATAGGCGTC CAGATTTGGA AATCTATAAG 
CCTGGACTTT CTCGACTAAG GAACAGACCT AAAACTAAGG AGGCTTCTGG GAATGAGGAA 
TTTAAGGATG AGATAGTAAA TGACAGAGAT TCTTCTGCTG TTGGGAATGA TACACAGCTT 
ATTCAAGTTT GCAAGGAATT GGACAGCCAA CAGCAAAATG GCCCTATAGA TGCAGAAAAT 
AGTCAGGCAC AGGAAACTTT TCCAAAGACT GTTGGACTAG AGGACCGAAG TCTAAAGATC 
ATCAAAAGAA GCAAGAAACC AGACCTGCAG ATCTACCAGC CTGGAAGGCG GTTGCAGACC 
ATTACCAAAG AGTCAGCAGG CAGGGCAGAT GAAGAAGAGA TCCTCAACCA AGTGGAGCAG 
TTGAGGATAG AGGAGGATGA GTGTAAGGGA GAAGCTATAA AAGAAGAAGT TAATAACAAA 
CCGGATAAAA CCGAGATAGA AAAGCACCAA AGTAATGACC GTGTAAGAAC TGCCAAGGGA 
GAAAAGGGAA AAAAGATTGA AAAGGGGGAG GGGTCAAAGA AGGTGGCTGA TGACTCTGTC 
CCAGGAAAGC CTGGCTCTGT AAAGAGATAC TCGAGATCAG ACAAGAGAAG GAATCGCTAC 
CGCACTTGCA GTACCAGCTC AGCTGGTAGC AACAACAGTG CTGAGGGAGC TGGCCTGACC 
GATAATGGAT GTCGCCGGCG GCGCCAGGAT CGGGCCAAGG AGAGGCCACG ACTGAAGAAG 
CAGGTATCTT TGTCCTCAAC TGATTCCTTA GATGAGGACA GAGTTGATGA GCCTGATGTC 
CTAGGGTCCA GGAGGAGCTC AGAAAGGAAG AAGCATTTAG AAAGAAATTG GTCTGGCTGT 
GGTGAGGGTG AACAGAAAAG CAATGGTAAA GAAAACCGAA GTGCCCTTCG TGTCACTTTT 
GATGCAGAAA CCATGAGTAA AGACTCCCCT GTGGTGAGGT CAGTCAAAGA TAATGTGGAT 
AGAATGAAGT CTGACAAAGG CCCAAGCAGT GGGGGTAAGG GCTCTGAGAA GCAGGAGCTC 
AGACACCCGA GACAAGAACT TCGAGATCGT GGTCGTGGTA TCCTGATTCT GCCTGCCCAC 
ACTGCCCTAT CTGTCAGTTC ATCAGGTTCT CCGGAGTCTA CACCTTTGGG ACCTCGACTT 
TTATTTGGAT CTGGTAGTAA GGGATCTCGG AGTTGGGGCC GTGGAGGCAC TACCCGCCGA 
CTATGGGACC CAAACAATCC TGACCAGAAG CCTGCCCTAA AAAGCCAGAC ACCTCAGCTA 
CATTTCTTGG ACACTGATGA TGAAATTAGC CCTACATCTT GGGGTGATTC ACGTCAGGCC 
CAAGCATCTT ACTATAAATT TCAAAACTCT GATAACCCCT ATTATTACCC TCGGACACCA 
GGCCCTGCCT CTCAGTATCC CTATGCAGGC TATAGCCCTC TGCAGTACCC AGTAGGCCCT 
ACAAATGGTA TGTATCCAGG GGCTTACTAC CCGGGCTATC CTGCCCCCTC AGGACAATAT 
GTGTGTAGCC CTCTCCCTGC TAGCACCATG AGTCCTGAGG AAATAGAACA GCACGTGCGG 
AACATGCAGC AACAGGAGCT CCATCGGCTT CTCCGAGTGG CTGACAACCA GGAACTGCAG 
CTCAGCAACC TGCTTTCCAG GGACCGCATC AGCACTGAGG GCATGGAGAA GATGGCTCAG 
CTCAGAACTG AACTACTGCA GCTGTACGAG CGCTGTATTC TATTAGATAT TGAATTCTCC 
GACAGTCAGA ATGTAGATCA GATCCTGTGG AAGAACGCTT TCTATCAGGT GATTGAGAAG 
TTCAGGCAGC TTCTCAAGGA TCCAAACAGT GAGAACCCAG AGCAGATTCG GAACAGACTC 
TTGGAGCTCT TAGATGAGGG CAGTGACTTC TTTGATAGTT TGCTTCAGAA ACTACAGGTT 
ACTTACAAGT TCAAACTGGA GGACTACATG GATGGGCTTG CCATTCGAAG CAAGCCTTTA 
CGAAAAACAG TGAAATATGC CTTGATCAGT GCTCAACGAA GTATGATTTG CCAAGGAGAT 
ATCTCTAGGT ATCGGGAGCA AGCCAACGAT ACAGCAAACT ATGGGAAGGC TCGCAGTTGG 
TATCTGAAGG CTCAGCACAT TGCTCCCAAG AATGGGCGCC CCTATAACCA GTTGGCTCTA 
CTGGCAGTGT ATACGAGGAG GAAGCTTGAT GCTGTATATT ACTATATGCG CAGTTTAGCA 
GCCAGCAACC CTATCCTGAC TGCCAAGGAG AGTCTCATGA GTTTATTTGA AGAGACTAAA 
CGAAAGGCTG AACAGATGGA AAAGAAGCAG CATGAGGAAT TTGATATGAG CCCTGATAAG 
TGGCGAAAAG GAAAGAAGTC TACTTTCCGG CATGTTGGGG ATGACACTAC TCGCCTGGAG 
ATTTGGATTC ATCCATCACA TTCTCGGTCT GCCCAGGGCA CTGAGTCTGG GAAGGATTCT 
GAGCAGGAGA ATGGTCTGGG CAGCCTGAGT CCCAGTGATC TGAACAAAAG GTTCATCCTC 
AGTTTTCTCC ATGCCCATGG GAAGCTGTTT ACCCGGATTG GGATGGAGAC ATTCCCTGCA 
GTGGCTGAGA AGGTCCTTAA GGAGTTCCAA GTATTGCTGC AGCATAGCCC ATCTCCTATT 
GGAAGTACCC GTATGCTGCA GCTTATGACC ATCAACATGT TTGCCGTGCA TAACTCCCAG 
TTGAAAGACT GCTTCTCAGA GGAGTGTCGT TCTGTGATCC AGGAACAAGC TGCATCCTTG 
GGCTTGGCTA TGTTTTCTCT ACTGGTCCAG CGCTGCACAT GCCTACTTAA GGACTCTGCT 
AAAGCTCAGC TGTCCTCTCC TGAGGACCAA GAGGACCAAG ATGACATCAA GGTATCTTCC 
TTCGTCCCGG ACCTGAAGGA GCTGCTCCCC AGTGTGAAAG TCTGGTCTGA TTGGATGCTT 
GGCTACCCAG ACACCTGGAA TCCTCCACCC ACATCTTTGG ATCTGCCCTT GCAGGTTGCT 
GTGGATGTCT GGTCAACACT AGCTGATTTC TGTAACATAC TGACTGCAGT CAATCAGTCT 
GAGGTGCCAC TGTACAAGGA CCCAGATGAT GACCTCACCC TGCTTATCCT GGAAGAGGAT 
CGGCTTCTCT CTGGCTTTGT CCCCTTGCTG GCTGCCCCTC AGGACCCCTG CTACGTGGAG 
AAAACCTCGG ATAAGGTTAT TGCAGCTGAC TGCAAAAGGG TCACAGTGCT GAAGTATTTT 
CTGGAAGCCC TTTGTGGACA AGAAGAGCCT CTGCTGGCAT TCAAGGGTGG AAAATATGTG 
TCAGTGGCAC CCGTCCCAGA CACCATGGGA AAGGAAATGG GAAGCCAAGA GGGAAAACAA 
CTGGAAGATG AGGAAGAAGA TGTGGTGATT GAAGACTTTG AGGAAGATTC AGAGGCTGAA 
GGCAGTGGGG GTGAGGATGA CATCAGGGAA CTTCGGGCCA AGAAGCTGGC TCTAGCAAGG 
AAGATAGCTG AGCAGCAGCG TCGCCAGGAA AAGATCCAGG CTGTTTTGGA AGACCAGAGT 
CAGATGAGGC AGATGGAGCT GGAGATCAGA CCCTTGTTCC TCGTACCAGA TACCAACGGC 
TTCATTGACC ACCTGGCCAG TCTGGCTCGG CTGCTGGAGA GCAGGAAGTA CATCCTGGTG 
GTGCCCCTCA TCGTGATCAA TGAGCTGGAC GGCCTCGCCA AAGGGCAGGA GACAGACCAC 
CGGGCTGGGG GCTATGCCCG TGTGGTGCAA GAAAAGGCCC GAAAATCCAT TGAGTTCCTC 
GAACGGAGAT TTGAGAGTCG GGACTCCTGC CTGCGGGCCT TGACCAGCCG TGGCAATGAA 
CTTGAATCTA TCGCCTTCCG AAGTGAGGAC ATTACTGGAC AGCTGGGTAA CAACGATGAC 
CTGATCCTCT CTTGCTGCCT GCACTACTGC AAAGACAAGG CTAAGGATTA CATGCCCACC 
AGCAAAGAGG AGCCAATCCG CCTGTTACGG GAGGTGGTGT TGCTGACCGA TGATCGGAAC 
CTTCGTGTAA AGGCGCTGAC AAGGAATGTG CCTGTGAGGG ACATCCCAGC CTTCCTTACG 
TGGGCCCAGG TGGGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011246961.1
CDS	383..4600
Translation	MTWIQENTTPLYFHNSKNMKELKEPRQRKDNRRPDLEIYKPGLSRLRNRPKTKEASGNEEFKDEIVNDRDSSAVGNDTQLIQVCKELDSQQQNGPIDAENSQAQETFPKTVGLEDRSLKIIKRSKKPDLQIYQPGRRLQTITKESAGRADEEEILNQVEQLRIEEDECKGEAIKEEVNNKPDKTEIEKHQSNDRVRTAKGEKGKKIEKGEGSKKVADDSVPGKPGSVKRYSRSDKRRNRYRTCSTSSAGSNNSAEGAGLTDNGCRRRRQDRAKERPRLKKQVSLSSTDSLDEDRVDEPDVLGSRRSSERKKHLERNWSGCGEGEQKSNGKENRSALRVTFDAETMSKDSPVVRSVKDNVDRMKSDKGPSSGGKGSEKQELRHPRQELRDRGRGILILPAHTALSVSSSGSPESTPLGPRLLFGSGSKGSRSWGRGGTTRRLWDPNNPDQKPALKSQTPQLHFLDTDDEISPTSWGDSRQAQASYYKFQNSDNPYYYPRTPGPASQYPYAGYSPLQYPVGPTNGMYPGAYYPGYPAPSGQYVCSPLPASTMSPEEIEQHVRNMQQQELHRLLRVADNQELQLSNLLSRDRISTEGMEKMAQLRTELLQLYERCILLDIEFSDSQNVDQILWKNAFYQVIEKFRQLLKDPNSENPEQIRNRLLELLDEGSDFFDSLLQKLQVTYKFKLEDYMDGLAIRSKPLRKTVKYALISAQRSMICQGDISRYREQANDTANYGKARSWYLKAQHIAPKNGRPYNQLALLAVYTRRKLDAVYYYMRSLAASNPILTAKESLMSLFEETKRKAEQMEKKQHEEFDMSPDKWRKGKKSTFRHVGDDTTRLEIWIHPSHSRSAQGTESGKDSEQENGLGSLSPSDLNKRFILSFLHAHGKLFTRIGMETFPAVAEKVLKEFQVLLQHSPSPIGSTRMLQLMTINMFAVHNSQLKDCFSEECRSVIQEQAASLGLAMFSLLVQRCTCLLKDSAKAQLSSPEDQEDQDDIKVSSFVPDLKELLPSVKVWSDWMLGYPDTWNPPPTSLDLPLQVAVDVWSTLADFCNILTAVNQSEVPLYKDPDDDLTLLILEEDRLLSGFVPLLAAPQDPCYVEKTSDKVIAADCKRVTVLKYFLEALCGQEEPLLAFKGGKYVSVAPVPDTMGKEMGSQEGKQLEDEEEDVVIEDFEEDSEAEGSGGEDDIRELRAKKLALARKIAEQQRRQEKIQAVLEDQSQMRQMELEIRPLFLVPDTNGFIDHLASLARLLESRKYILVVPLIVINELDGLAKGQETDHRAGGYARVVQEKARKSIEFLERRFESRDSCLRALTSRGNELESIAFRSEDITGQLGNNDDLILSCCLHYCKDKAKDYMPTSKEEPIRLLREVVLLTDDRNLRVKALTRNVPVRDIPAFLTWAQVG

Target ORF information:

RefSeq Version	XM_011248659.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus Smg-6 homolog, nonsense mediated mRNA decay factor (C. elegans) (Smg6), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011248659.2

ORF Insert Sequence:

ATGACTTGGA TACAAGAGAA TACAACTCCC CTCTATTTCC ATAACTCAAA AAACATGAAG 
GAACTAAAGG AACCCAGACA GCGCAAAGAT AATAGGCGTC CAGATTTGGA AATCTATAAG 
CCTGGACTTT CTCGACTAAG GAACAGACCT AAAACTAAGG AGGCTTCTGG GAATGAGGAA 
TTTAAGGATG AGATAGTAAA TGACAGAGAT TCTTCTGCTG TTGGGAATGA TACACAGCTT 
ATTCAAGTTT GCAAGGAATT GGACAGCCAA CAGCAAAATG GCCCTATAGA TGCAGAAAAT 
AGTCAGGCAC AGGAAACTTT TCCAAAGACT GTTGGACTAG AGGACCGAAG TCTAAAGATC 
ATCAAAAGAA GCAAGAAACC AGACCTGCAG ATCTACCAGC CTGGAAGGCG GTTGCAGACC 
ATTACCAAAG AGTCAGCAGG CAGGGCAGAT GAAGAAGAGA TCCTCAACCA AGTGGAGCAG 
TTGAGGATAG AGGAGGATGA GTGTAAGGGA GAAGCTATAA AAGAAGAAGT TAATAACAAA 
CCGGATAAAA CCGAGATAGA AAAGCACCAA AGTAATGACC GTGTAAGAAC TGCCAAGGGA 
GAAAAGGGAA AAAAGATTGA AAAGGGGGAG GGGTCAAAGA AGGTGGCTGA TGACTCTGTC 
CCAGGAAAGC CTGGCTCTGT AAAGAGATAC TCGAGATCAG ACAAGAGAAG GAATCGCTAC 
CGCACTTGCA GTACCAGCTC AGCTGGTAGC AACAACAGTG CTGAGGGAGC TGGCCTGACC 
GATAATGGAT GTCGCCGGCG GCGCCAGGAT CGGGCCAAGG AGAGGCCACG ACTGAAGAAG 
CAGGTATCTT TGTCCTCAAC TGATTCCTTA GATGAGGACA GAGTTGATGA GCCTGATGTC 
CTAGGGTCCA GGAGGAGCTC AGAAAGGAAG AAGCATTTAG AAAGAAATTG GTCTGGCTGT 
GGTGAGGGTG AACAGAAAAG CAATGGTAAA GAAAACCGAA GTGCCCTTCG TGTCACTTTT 
GATGCAGAAA CCATGAGTAA AGACTCCCCT GTGGTGAGGT CAGTCAAAGA TAATGTGGAT 
AGAATGAAGT CTGACAAAGG CCCAAGCAGT GGGGGTAAGG GCTCTGAGAA GCAGGAGCTC 
AGACACCCGA GACAAGAACT TCGAGATCGT GGTCGTGGTA TCCTGATTCT GCCTGCCCAC 
ACTGCCCTAT CTGTCAGTTC ATCAGGTTCT CCGGAGTCTA CACCTTTGGG ACCTCGACTT 
TTATTTGGAT CTGGTAGTAA GGGATCTCGG AGTTGGGGCC GTGGAGGCAC TACCCGCCGA 
CTATGGGACC CAAACAATCC TGACCAGAAG CCTGCCCTAA AAAGCCAGAC ACCTCAGCTA 
CATTTCTTGG ACACTGATGA TGAAATTAGC CCTACATCTT GGGGTGATTC ACGTCAGGCC 
CAAGCATCTT ACTATAAATT TCAAAACTCT GATAACCCCT ATTATTACCC TCGGACACCA 
GGCCCTGCCT CTCAGTATCC CTATGCAGGC TATAGCCCTC TGCAGTACCC AGTAGGCCCT 
ACAAATGGTA TGTATCCAGG GGCTTACTAC CCGGGCTATC CTGCCCCCTC AGGACAATAT 
GTGTGTAGCC CTCTCCCTGC TAGCACCATG AGTCCTGAGG AAATAGAACA GCACGTGCGG 
AACATGCAGC AACAGGAGCT CCATCGGCTT CTCCGAGTGG CTGACAACCA GGAACTGCAG 
CTCAGCAACC TGCTTTCCAG GGACCGCATC AGCACTGAGG GCATGGAGAA GATGGCTCAG 
CTCAGAACTG AACTACTGCA GCTGTACGAG CGCTGTATTC TATTAGATAT TGAATTCTCC 
GACAGTCAGA ATGTAGATCA GATCCTGTGG AAGAACGCTT TCTATCAGGT GATTGAGAAG 
TTCAGGCAGC TTCTCAAGGA TCCAAACAGT GAGAACCCAG AGCAGATTCG GAACAGACTC 
TTGGAGCTCT TAGATGAGGG CAGTGACTTC TTTGATAGTT TGCTTCAGAA ACTACAGGTT 
ACTTACAAGT TCAAACTGGA GGACTACATG GATGGGCTTG CCATTCGAAG CAAGCCTTTA 
CGAAAAACAG TGAAATATGC CTTGATCAGT GCTCAACGAA GTATGATTTG CCAAGGAGAT 
ATCTCTAGGT ATCGGGAGCA AGCCAACGAT ACAGCAAACT ATGGGAAGGC TCGCAGTTGG 
TATCTGAAGG CTCAGCACAT TGCTCCCAAG AATGGGCGCC CCTATAACCA GTTGGCTCTA 
CTGGCAGTGT ATACGAGGAG GAAGCTTGAT GCTGTATATT ACTATATGCG CAGTTTAGCA 
GCCAGCAACC CTATCCTGAC TGCCAAGGAG AGTCTCATGA GTTTATTTGA AGAGACTAAA 
CGAAAGGCTG AACAGATGGA AAAGAAGCAG CATGAGGAAT TTGATATGAG CCCTGATAAG 
TGGCGAAAAG GAAAGAAGTC TACTTTCCGG CATGTTGGGG ATGACACTAC TCGCCTGGAG 
ATTTGGATTC ATCCATCACA TTCTCGGTCT GCCCAGGGCA CTGAGTCTGG GAAGGATTCT 
GAGCAGGAGA ATGGTCTGGG CAGCCTGAGT CCCAGTGATC TGAACAAAAG GTTCATCCTC 
AGTTTTCTCC ATGCCCATGG GAAGCTGTTT ACCCGGATTG GGATGGAGAC ATTCCCTGCA 
GTGGCTGAGA AGGTCCTTAA GGAGTTCCAA GTATTGCTGC AGCATAGCCC ATCTCCTATT 
GGAAGTACCC GTATGCTGCA GCTTATGACC ATCAACATGT TTGCCGTGCA TAACTCCCAG 
TTGAAAGACT GCTTCTCAGA GGAGTGTCGT TCTGTGATCC AGGAACAAGC TGCATCCTTG 
GGCTTGGCTA TGTTTTCTCT ACTGGTCCAG CGCTGCACAT GCCTACTTAA GGACTCTGCT 
AAAGCTCAGC TGTCCTCTCC TGAGGACCAA GAGGACCAAG ATGACATCAA GGTATCTTCC 
TTCGTCCCGG ACCTGAAGGA GCTGCTCCCC AGTGTGAAAG TCTGGTCTGA TTGGATGCTT 
GGCTACCCAG ACACCTGGAA TCCTCCACCC ACATCTTTGG ATCTGCCCTT GCAGGTTGCT 
GTGGATGTCT GGTCAACACT AGCTGATTTC TGTAACATAC TGACTGCAGT CAATCAGTCT 
GAGGTGCCAC TGTACAAGGA CCCAGATGAT GACCTCACCC TGCTTATCCT GGAAGAGGAT 
CGGCTTCTCT CTGGCTTTGT CCCCTTGCTG GCTGCCCCTC AGGACCCCTG CTACGTGGAG 
AAAACCTCGG ATAAGGTTAT TGCAGCTGAC TGCAAAAGGG TCACAGTGCT GAAGTATTTT 
CTGGAAGCCC TTTGTGGACA AGAAGAGCCT CTGCTGGCAT TCAAGGGTGG AAAATATGTG 
TCAGTGGCAC CCGTCCCAGA CACCATGGGA AAGGAAATGG GAAGCCAAGA GGGAAAACAA 
CTGGAAGATG AGGAAGAAGA TGTGGTGATT GAAGACTTTG AGGAAGATTC AGAGGCTGAA 
GGCAGTGGGG GTGAGGATGA CATCAGGGAA CTTCGGGCCA AGAAGCTGGC TCTAGCAAGG 
AAGATAGCTG AGCAGCAGCG TCGCCAGGAA AAGATCCAGG CTGTTTTGGA AGACCAGAGT 
CAGATGAGGC AGATGGAGCT GGAGATCAGA CCCTTGTTCC TCGTACCAGA TACCAACGGC 
TTCATTGACC ACCTGGCCAG TCTGGCTCGG CTGCTGGAGA GCAGGAAGTA CATCCTGGTG 
GTGCCCCTCA TCGTGATCAA TGAGCTGGAC GGCCTCGCCA AAGGGCAGGA GACAGACCAC 
CGGGCTGGGG GCTATGCCCG TGTGGTGCAA GAAAAGGCCC GAAAATCCAT TGAGTTCCTC 
GAACGGAGAT TTGAGAGTCG GGACTCCTGC CTGCGGGCCT TGACCAGCCG TGGCAATGAA 
CTTGAATCTA TCGCCTTCCG AAGTGAGGAC ATTACTGGAC AGCTGGGTAA CAACGATGAC 
CTGATCCTCT CTTGCTGCCT GCACTACTGC AAAGACAAGG CTAAGGATTA CATGCCCACC 
AGCAAAGAGG AGCCAATCCG CCTGTTACGG GAGGTGGTGT TGCTGACCGA TGATCGGAAC 
CTTCGTGTAA AGGCGCTGAC AAGGAATGTG CCTGTGAGGG ACATCCCAGC CTTCCTTACG 
TGGGCCCAGG TGGGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Smg6/Est1 licenses embryonic stem cell differentiation via nonsense-mediated mRNA decay.
The EMBO journal34(12)1630-47(2015 Jun)
Li T,Shi Y,Wang P,Guachalla LM,Sun B,Joerss T,Chen YS,Groth M,Krueger A,Platzer M,Yang YG,Rudolph KL,Wang ZQ

Characterization of an exchangeable gene trap using pU-17 carrying a stop codon-beta geo cassette.
Development, growth & differentiation47(3)163-72(2005 Apr)
Taniwaki T,Haruna K,Nakamura H,Sekimoto T,Oike Y,Imaizumi T,Saito F,Muta M,Soejima Y,Utoh A,Nakagata N,Araki M,Yamamura K,Araki K

Tagging genes with cassette-exchange sites.
Nucleic acids research33(4)e44(2005 Mar)
Cobellis G,Nicolaus G,Iovino M,Romito A,Marra E,Barbarisi M,Sardiello M,Di Giorgio FP,Iovino N,Zollo M,Ballabio A,Cortese R

Prediction of the coding sequences of mouse homologues of KIAA gene: III. the complete nucleotide sequences of 500 mouse KIAA-homologous cDNAs identified by screening of terminal sequences of cDNA clones randomly sampled from size-fractionated libraries.
DNA research : an international journal for rapid publication of reports on genes and genomes10(4)167-80(2003 Aug)
Okazaki N,Kikuno R,Ohara R,Inamoto S,Koseki H,Hiraoka S,Saga Y,Nagase T,Ohara O,Koga H

BayGenomics: a resource of insertional mutations in mouse embryonic stem cells.
Nucleic acids research31(1)278-81(2003 Jan)
Stryke D,Kawamoto M,Huang CC,Johns SJ,King LA,Harper CA,Meng EC,Lee RE,Yee A,L'Italien L,Chuang PT,Young SG,Skarnes WC,Babbitt PC,Ferrin TE

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Smg6 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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