Chpf2 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Chpf2
Entrez Gene ID	100910
Full Name	chondroitin polymerizing factor 2
Synonyms	2010209O12Rik,AW060945,mKIAA1402,mir-671
General protein information	Preferred Names chondroitin polymerizing factor 2 Names chondroitin sulfate glucuronyltransferase
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 5 Map Location: 5\|5 A3

mRNA and Protein(s)

mRNA	Protein	Name
XM_017320583.1	XP_017176072.1	chondroitin sulfate glucuronyltransferase isoform X1
NM_133913.2	NP_598674.2	chondroitin sulfate glucuronyltransferase precursor

Pathways from BioSystems

Homologies

Rattus norvegicus (Norway rat)	Chpf2	NP_001100044.1
Homo sapiens (human)	CHPF2	NP_001271224.1
Canis lupus familiaris (dog)	CHPF2	XP_539923.4
Mus musculus (house mouse)	Chpf2	NP_598674.2
Bos taurus (cattle)	CHPF2	NP_001193852.1
Gallus gallus (chicken)	CHPF2	XP_423717.3
Pan troglodytes (chimpanzee)	CHPF2	XP_003951246.1
Danio rerio (zebrafish)	chpf2	XP_001337205.1

Gene Ontology
Bibliography

Related articles in PubMed

miRBase: integrating microRNA annotation and deep-sequencing data.
Kozomara A, Griffiths-Jones S
Nucleic acids research39(Database issue)D152-7(2011 Jan)

A mouse knockout library for secreted and transmembrane proteins.
Tang T, Li L, Tang J, Li Y, Lin WY, Martin F, Grant D, Solloway M, Parker L, Ye W, Forrest W, Ghilardi N, Oravecz T, Platt KA, Rice DS, Hansen GM, Abuin A, Eberhart DE, Godowski P, Holt KH, Peterson A, Zambrowicz BP, de Sauvage FJ
Nature biotechnology28(7)749-55(2010 Jul)

Prediction of the coding sequences of mouse homologues of KIAA gene: II. The complete nucleotide sequences of 400 mouse KIAA-homologous cDNAs identified by screening of terminal sequences of cDNA clones randomly sampled from size-fractionated libraries.
Okazaki N, Kikuno R, Ohara R, Inamoto S, Aizawa H, Yuasa S, Nakajima D, Nagase T, Ohara O, Koga H
DNA research : an international journal for rapid publication of reports on genes and genomes10(1)35-48(2003 Feb)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Chpf2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Chpf2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu10350	NM_133913.2 Latest version!	Mus musculus chondroitin polymerizing factor 2 (Chpf2), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$447.30 $639.00
OMu10350	XM_017320583.1 Latest version!	Mus musculus chondroitin polymerizing factor 2 (Chpf2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$447.30 $639.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Chpf2 ( NM_133913.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_598674.2 Mus musculus chondroitin polymerizing factor 2 (Chpf2), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OMu10350
Clone ID Related Accession (Same CDS sequence)	XM_017320583.1 , NM_133913.2
Accession Version	NM_133913.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2307bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-04-05
Organism	Mus musculus(house mouse)
Product	chondroitin sulfate glucuronyltransferase precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK122504.1 and BC036970.1. On Oct 26, 2007 this sequence version replaced NM_133913.1. ##Evidence-Data-START## Transcript exon combination :: SRR1660813.206649.1, SRR1660811.188527.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END##

ATGCGACTGA GCTCCCTGCT GGCTCTGCTG CGGCCAGTGC TCCCCCTCAT CCTAGGACTG 
TCTCTGGGAT GCAGCCTGAG CCTCCTGCGG GTTTCCTGGA TCCAGGGTGA GGGAGAAGAT 
CCCTGTGTAG AGGCTGTGGG GAAGCCAGGA GGGCCACAGA ATCCAGACTC AAAAAATGGG 
CTGGACCAAG GCGATGAAGA CTTCAGACCC AGGATTGTGC CTTACTACAG GGATCCTAAC 
AAGCCCTACA AGAAGGTGCT CAGGACTCGG TATATACAGA CAGAGCTGGG CTCTCGTGAG 
CGCCTGCTGG TGGCTGTCCT GACTTCTCGA GCCACACTGT CCACCCTGGC CGTCGCGGTT 
AACCGTACAG TAGCACATCA CTTCCCTCGG TTACTCTACT TCACAGGGCA GCGAGGGGCC 
CGGGCTCCTG CAGGGATGCA GGTGGTGTCT CATGGAGATG AGCGGCCTGC CTGGCTCATG 
TCAGAGACCC TGCGCCACCT TCACACACAC TTTGGGGCAG ACTATGACTG GTTCTTCATC 
ATGCAGGACG ACACATATGT GCAGGCCCCC AGATTGGCAG CTCTTGCTGG TCACCTTAGC 
ATCAACCAAG ACCTGTACTT AGGCCATGCA GAGGAATTCA TTGGTACAGG AGAGCAGGCC 
CGGTACTGCC ATGGGGGCTT TGGCTACCTG TTGTCACGGA GTCTCCTATT GCGCTTGCGG 
CCACATCTGG ATGGCTGCCG AGGGGACATT CTCAGTGCCC GGCCTGATGA ATGGCTTGGC 
CGCTGCCTCA TCGACACTCT GGGCATCGGC TGTGTCTCAC AGCACCAGGG GCAACAGTAT 
CGCTCCTTCG AACTGGCCAA GAATAGGGAC CCTGAGAAGG AGGAGAGCCC TGCTTTCCTG 
AGTGCCTTCG CCGTTCACCC GGTCCCCGAG GGCACCCTCA TGTACCGGCT CCACAAACGC 
TTCAGTGCTC TGGAGTTGGA GCGGGCTTAC AGCGAAATAG AACAACTGCA GGCCCAGATC 
CGGAACCTGA CGGTGCTGAC CCCTGAGGGG GAGGCAGGGC TAAGCTGGCC TGTTGGGCTC 
CCGGCCCCTT TCACTCCACA CTCTCGCTTC GAGGTGCTGG GCTGGGACTA CTTCACGGAG 
CAACACACCT TCGCCTGTGC AGACGGGGCT CCCAAGTGTC CCCTGCAGGG GGCTAGCAGG 
GCAGATGTAG GGGACGCTGT GGACACAGCT CTGGAGCAGC TCAATCGGCG CTATCAGCCC 
CGCCTGCGCT TCCGGAAGCA GCGGCTTCTC AATGGCTACC GGCGCTTTGA CCCTGCAAGG 
GGCATGGAAT ACACCCTCGA CTTGCTGCTG GAAGCTGTGA CTCAGCGAGG GCACAGACGC 
TCCCTGGCCC GCAGAGTGAG CCTGCTGAGG CCACTGAGCA GAGTGGAAAT CCTGCCTATG 
CCCTATGTCA CCGAGGCTAC CCGGGTGCAA CTGGTGCTGC CACTCCTGGT GGCTGAAGCT 
CCGGCCGCCT TGGCTTTTCT TGAGGCCTTT GCTGCCAGTG TTCTGGAGCC TCGAGAACAT 
GCATTGCTCA CCCTGTTGTT GGTCTATGGG CCCCGGGAAG GCCGAGGGGG CCCTGACCCA 
TTTCTCGGAG TGAAGGCTGC AGCAGCTGAG TTAGAACGAC GGTACCCAGG GGCGAGGCTG 
GCCTGGCTCG CTGTGCGAGC AGAGGCCCCT TCGCAGGTGC GACTCATGGA TGTCATCTCC 
AAGAAACACC CTGTGGACAC ACTCTTCTTC CTTACCACCG TGTGGACAAG ACCTGGGTCG 
GAAGTCCTCA ACCGCTGCCG TATGAATGCA ATCTCGGGCT GGCAGGCCTT CTTTCCGGTC 
CACTTTCAGG AGTTCAACCC TGCTCTGTCA CCACAGAGAT CTCCCCCAGG TGTCCCTGGG 
GCTGATCCCC CCTCCCCTGG TGTGGACCCT TCTAGGGGGG CTCCGGTCGG GGGCAGATTT 
GACCGGCAGG CTTCAGCAGA GGGCTGCTTC TACAATGCTG ACTACCTGGC AGCCCGCGCC 
CGGCTGGCTG GCGAACTGGC AGGCCAGGAA GAGGAGGAAG CCCTGGAGGG GCTGGAGGTG 
ATGGATGTTT TCCTCCGGTT CTCAGGGCTC CACCTCTTTC GAGCCGTAGA GCCAGGGCTG 
GTGCAGAAGT TCTCTCTGCG GGACTGTAGC CCCCGGCTCA GCGAGGAACT GTACCACCGT 
TGTCGCCTCA GCAATCTGGA GGGGCTGGGG GCCCGTACCC AGCTAGCCAT GGCTCTGTTT 
GAACAGGAAC AGGCCAACAG CACTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_598674.2
CDS	1289..3595
Translation	MRLSSLLALLRPVLPLILGLSLGCSLSLLRVSWIQGEGEDPCVEAVGKPGGPQNPDSKNGLDQGDEDFRPRIVPYYRDPNKPYKKVLRTRYIQTELGSRERLLVAVLTSRATLSTLAVAVNRTVAHHFPRLLYFTGQRGARAPAGMQVVSHGDERPAWLMSETLRHLHTHFGADYDWFFIMQDDTYVQAPRLAALAGHLSINQDLYLGHAEEFIGTGEQARYCHGGFGYLLSRSLLLRLRPHLDGCRGDILSARPDEWLGRCLIDTLGIGCVSQHQGQQYRSFELAKNRDPEKEESPAFLSAFAVHPVPEGTLMYRLHKRFSALELERAYSEIEQLQAQIRNLTVLTPEGEAGLSWPVGLPAPFTPHSRFEVLGWDYFTEQHTFACADGAPKCPLQGASRADVGDAVDTALEQLNRRYQPRLRFRKQRLLNGYRRFDPARGMEYTLDLLLEAVTQRGHRRSLARRVSLLRPLSRVEILPMPYVTEATRVQLVLPLLVAEAPAALAFLEAFAASVLEPREHALLTLLLVYGPREGRGGPDPFLGVKAAAAELERRYPGARLAWLAVRAEAPSQVRLMDVISKKHPVDTLFFLTTVWTRPGSEVLNRCRMNAISGWQAFFPVHFQEFNPALSPQRSPPGVPGADPPSPGVDPSRGAPVGGRFDRQASAEGCFYNADYLAARARLAGELAGQEEEEALEGLEVMDVFLRFSGLHLFRAVEPGLVQKFSLRDCSPRLSEELYHRCRLSNLEGLGARTQLAMALFEQEQANST

Target ORF information:

RefSeq Version	NM_133913.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus chondroitin polymerizing factor 2 (Chpf2), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_133913.2

ORF Insert Sequence:

ATGCGACTGA GCTCCCTGCT GGCTCTGCTG CGGCCAGTGC TCCCCCTCAT CCTAGGACTG 
TCTCTGGGAT GCAGCCTGAG CCTCCTGCGG GTTTCCTGGA TCCAGGGTGA GGGAGAAGAT 
CCCTGTGTAG AGGCTGTGGG GAAGCCAGGA GGGCCACAGA ATCCAGACTC AAAAAATGGG 
CTGGACCAAG GCGATGAAGA CTTCAGACCC AGGATTGTGC CTTACTACAG GGATCCTAAC 
AAGCCCTACA AGAAGGTGCT CAGGACTCGG TATATACAGA CAGAGCTGGG CTCTCGTGAG 
CGCCTGCTGG TGGCTGTCCT GACTTCTCGA GCCACACTGT CCACCCTGGC CGTCGCGGTT 
AACCGTACAG TAGCACATCA CTTCCCTCGG TTACTCTACT TCACAGGGCA GCGAGGGGCC 
CGGGCTCCTG CAGGGATGCA GGTGGTGTCT CATGGAGATG AGCGGCCTGC CTGGCTCATG 
TCAGAGACCC TGCGCCACCT TCACACACAC TTTGGGGCAG ACTATGACTG GTTCTTCATC 
ATGCAGGACG ACACATATGT GCAGGCCCCC AGATTGGCAG CTCTTGCTGG TCACCTTAGC 
ATCAACCAAG ACCTGTACTT AGGCCATGCA GAGGAATTCA TTGGTACAGG AGAGCAGGCC 
CGGTACTGCC ATGGGGGCTT TGGCTACCTG TTGTCACGGA GTCTCCTATT GCGCTTGCGG 
CCACATCTGG ATGGCTGCCG AGGGGACATT CTCAGTGCCC GGCCTGATGA ATGGCTTGGC 
CGCTGCCTCA TCGACACTCT GGGCATCGGC TGTGTCTCAC AGCACCAGGG GCAACAGTAT 
CGCTCCTTCG AACTGGCCAA GAATAGGGAC CCTGAGAAGG AGGAGAGCCC TGCTTTCCTG 
AGTGCCTTCG CCGTTCACCC GGTCCCCGAG GGCACCCTCA TGTACCGGCT CCACAAACGC 
TTCAGTGCTC TGGAGTTGGA GCGGGCTTAC AGCGAAATAG AACAACTGCA GGCCCAGATC 
CGGAACCTGA CGGTGCTGAC CCCTGAGGGG GAGGCAGGGC TAAGCTGGCC TGTTGGGCTC 
CCGGCCCCTT TCACTCCACA CTCTCGCTTC GAGGTGCTGG GCTGGGACTA CTTCACGGAG 
CAACACACCT TCGCCTGTGC AGACGGGGCT CCCAAGTGTC CCCTGCAGGG GGCTAGCAGG 
GCAGATGTAG GGGACGCTGT GGACACAGCT CTGGAGCAGC TCAATCGGCG CTATCAGCCC 
CGCCTGCGCT TCCGGAAGCA GCGGCTTCTC AATGGCTACC GGCGCTTTGA CCCTGCAAGG 
GGCATGGAAT ACACCCTCGA CTTGCTGCTG GAAGCTGTGA CTCAGCGAGG GCACAGACGC 
TCCCTGGCCC GCAGAGTGAG CCTGCTGAGG CCACTGAGCA GAGTGGAAAT CCTGCCTATG 
CCCTATGTCA CCGAGGCTAC CCGGGTGCAA CTGGTGCTGC CACTCCTGGT GGCTGAAGCT 
CCGGCCGCCT TGGCTTTTCT TGAGGCCTTT GCTGCCAGTG TTCTGGAGCC TCGAGAACAT 
GCATTGCTCA CCCTGTTGTT GGTCTATGGG CCCCGGGAAG GCCGAGGGGG CCCTGACCCA 
TTTCTCGGAG TGAAGGCTGC AGCAGCTGAG TTAGAACGAC GGTACCCAGG GGCGAGGCTG 
GCCTGGCTCG CTGTGCGAGC AGAGGCCCCT TCGCAGGTGC GACTCATGGA TGTCATCTCC 
AAGAAACACC CTGTGGACAC ACTCTTCTTC CTTACCACCG TGTGGACAAG ACCTGGGTCG 
GAAGTCCTCA ACCGCTGCCG TATGAATGCA ATCTCGGGCT GGCAGGCCTT CTTTCCGGTC 
CACTTTCAGG AGTTCAACCC TGCTCTGTCA CCACAGAGAT CTCCCCCAGG TGTCCCTGGG 
GCTGATCCCC CCTCCCCTGG TGTGGACCCT TCTAGGGGGG CTCCGGTCGG GGGCAGATTT 
GACCGGCAGG CTTCAGCAGA GGGCTGCTTC TACAATGCTG ACTACCTGGC AGCCCGCGCC 
CGGCTGGCTG GCGAACTGGC AGGCCAGGAA GAGGAGGAAG CCCTGGAGGG GCTGGAGGTG 
ATGGATGTTT TCCTCCGGTT CTCAGGGCTC CACCTCTTTC GAGCCGTAGA GCCAGGGCTG 
GTGCAGAAGT TCTCTCTGCG GGACTGTAGC CCCCGGCTCA GCGAGGAACT GTACCACCGT 
TGTCGCCTCA GCAATCTGGA GGGGCTGGGG GCCCGTACCC AGCTAGCCAT GGCTCTGTTT 
GAACAGGAAC AGGCCAACAG CACTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Chpf2 ( XM_017320583.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017176072.1 Mus musculus chondroitin polymerizing factor 2 (Chpf2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu10350
Clone ID Related Accession (Same CDS sequence)	XM_017320583.1 , NM_133913.2
Accession Version	XM_017320583.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2307bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	chondroitin sulfate glucuronyltransferase isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000071.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGACTGA GCTCCCTGCT GGCTCTGCTG CGGCCAGTGC TCCCCCTCAT CCTAGGACTG 
TCTCTGGGAT GCAGCCTGAG CCTCCTGCGG GTTTCCTGGA TCCAGGGTGA GGGAGAAGAT 
CCCTGTGTAG AGGCTGTGGG GAAGCCAGGA GGGCCACAGA ATCCAGACTC AAAAAATGGG 
CTGGACCAAG GCGATGAAGA CTTCAGACCC AGGATTGTGC CTTACTACAG GGATCCTAAC 
AAGCCCTACA AGAAGGTGCT CAGGACTCGG TATATACAGA CAGAGCTGGG CTCTCGTGAG 
CGCCTGCTGG TGGCTGTCCT GACTTCTCGA GCCACACTGT CCACCCTGGC CGTCGCGGTT 
AACCGTACAG TAGCACATCA CTTCCCTCGG TTACTCTACT TCACAGGGCA GCGAGGGGCC 
CGGGCTCCTG CAGGGATGCA GGTGGTGTCT CATGGAGATG AGCGGCCTGC CTGGCTCATG 
TCAGAGACCC TGCGCCACCT TCACACACAC TTTGGGGCAG ACTATGACTG GTTCTTCATC 
ATGCAGGACG ACACATATGT GCAGGCCCCC AGATTGGCAG CTCTTGCTGG TCACCTTAGC 
ATCAACCAAG ACCTGTACTT AGGCCATGCA GAGGAATTCA TTGGTACAGG AGAGCAGGCC 
CGGTACTGCC ATGGGGGCTT TGGCTACCTG TTGTCACGGA GTCTCCTATT GCGCTTGCGG 
CCACATCTGG ATGGCTGCCG AGGGGACATT CTCAGTGCCC GGCCTGATGA ATGGCTTGGC 
CGCTGCCTCA TCGACACTCT GGGCATCGGC TGTGTCTCAC AGCACCAGGG GCAACAGTAT 
CGCTCCTTCG AACTGGCCAA GAATAGGGAC CCTGAGAAGG AGGAGAGCCC TGCTTTCCTG 
AGTGCCTTCG CCGTTCACCC GGTCCCCGAG GGCACCCTCA TGTACCGGCT CCACAAACGC 
TTCAGTGCTC TGGAGTTGGA GCGGGCTTAC AGCGAAATAG AACAACTGCA GGCCCAGATC 
CGGAACCTGA CGGTGCTGAC CCCTGAGGGG GAGGCAGGGC TAAGCTGGCC TGTTGGGCTC 
CCGGCCCCTT TCACTCCACA CTCTCGCTTC GAGGTGCTGG GCTGGGACTA CTTCACGGAG 
CAACACACCT TCGCCTGTGC AGACGGGGCT CCCAAGTGTC CCCTGCAGGG GGCTAGCAGG 
GCAGATGTAG GGGACGCTGT GGACACAGCT CTGGAGCAGC TCAATCGGCG CTATCAGCCC 
CGCCTGCGCT TCCGGAAGCA GCGGCTTCTC AATGGCTACC GGCGCTTTGA CCCTGCAAGG 
GGCATGGAAT ACACCCTCGA CTTGCTGCTG GAAGCTGTGA CTCAGCGAGG GCACAGACGC 
TCCCTGGCCC GCAGAGTGAG CCTGCTGAGG CCACTGAGCA GAGTGGAAAT CCTGCCTATG 
CCCTATGTCA CCGAGGCTAC CCGGGTGCAA CTGGTGCTGC CACTCCTGGT GGCTGAAGCT 
CCGGCCGCCT TGGCTTTTCT TGAGGCCTTT GCTGCCAGTG TTCTGGAGCC TCGAGAACAT 
GCATTGCTCA CCCTGTTGTT GGTCTATGGG CCCCGGGAAG GCCGAGGGGG CCCTGACCCA 
TTTCTCGGAG TGAAGGCTGC AGCAGCTGAG TTAGAACGAC GGTACCCAGG GGCGAGGCTG 
GCCTGGCTCG CTGTGCGAGC AGAGGCCCCT TCGCAGGTGC GACTCATGGA TGTCATCTCC 
AAGAAACACC CTGTGGACAC ACTCTTCTTC CTTACCACCG TGTGGACAAG ACCTGGGTCG 
GAAGTCCTCA ACCGCTGCCG TATGAATGCA ATCTCGGGCT GGCAGGCCTT CTTTCCGGTC 
CACTTTCAGG AGTTCAACCC TGCTCTGTCA CCACAGAGAT CTCCCCCAGG TGTCCCTGGG 
GCTGATCCCC CCTCCCCTGG TGTGGACCCT TCTAGGGGGG CTCCGGTCGG GGGCAGATTT 
GACCGGCAGG CTTCAGCAGA GGGCTGCTTC TACAATGCTG ACTACCTGGC AGCCCGCGCC 
CGGCTGGCTG GCGAACTGGC AGGCCAGGAA GAGGAGGAAG CCCTGGAGGG GCTGGAGGTG 
ATGGATGTTT TCCTCCGGTT CTCAGGGCTC CACCTCTTTC GAGCCGTAGA GCCAGGGCTG 
GTGCAGAAGT TCTCTCTGCG GGACTGTAGC CCCCGGCTCA GCGAGGAACT GTACCACCGT 
TGTCGCCTCA GCAATCTGGA GGGGCTGGGG GCCCGTACCC AGCTAGCCAT GGCTCTGTTT 
GAACAGGAAC AGGCCAACAG CACTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017176072.1
CDS	2048..4354
Translation	MRLSSLLALLRPVLPLILGLSLGCSLSLLRVSWIQGEGEDPCVEAVGKPGGPQNPDSKNGLDQGDEDFRPRIVPYYRDPNKPYKKVLRTRYIQTELGSRERLLVAVLTSRATLSTLAVAVNRTVAHHFPRLLYFTGQRGARAPAGMQVVSHGDERPAWLMSETLRHLHTHFGADYDWFFIMQDDTYVQAPRLAALAGHLSINQDLYLGHAEEFIGTGEQARYCHGGFGYLLSRSLLLRLRPHLDGCRGDILSARPDEWLGRCLIDTLGIGCVSQHQGQQYRSFELAKNRDPEKEESPAFLSAFAVHPVPEGTLMYRLHKRFSALELERAYSEIEQLQAQIRNLTVLTPEGEAGLSWPVGLPAPFTPHSRFEVLGWDYFTEQHTFACADGAPKCPLQGASRADVGDAVDTALEQLNRRYQPRLRFRKQRLLNGYRRFDPARGMEYTLDLLLEAVTQRGHRRSLARRVSLLRPLSRVEILPMPYVTEATRVQLVLPLLVAEAPAALAFLEAFAASVLEPREHALLTLLLVYGPREGRGGPDPFLGVKAAAAELERRYPGARLAWLAVRAEAPSQVRLMDVISKKHPVDTLFFLTTVWTRPGSEVLNRCRMNAISGWQAFFPVHFQEFNPALSPQRSPPGVPGADPPSPGVDPSRGAPVGGRFDRQASAEGCFYNADYLAARARLAGELAGQEEEEALEGLEVMDVFLRFSGLHLFRAVEPGLVQKFSLRDCSPRLSEELYHRCRLSNLEGLGARTQLAMALFEQEQANST

Target ORF information:

RefSeq Version	XM_017320583.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus chondroitin polymerizing factor 2 (Chpf2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017320583.1

ORF Insert Sequence:

ATGCGACTGA GCTCCCTGCT GGCTCTGCTG CGGCCAGTGC TCCCCCTCAT CCTAGGACTG 
TCTCTGGGAT GCAGCCTGAG CCTCCTGCGG GTTTCCTGGA TCCAGGGTGA GGGAGAAGAT 
CCCTGTGTAG AGGCTGTGGG GAAGCCAGGA GGGCCACAGA ATCCAGACTC AAAAAATGGG 
CTGGACCAAG GCGATGAAGA CTTCAGACCC AGGATTGTGC CTTACTACAG GGATCCTAAC 
AAGCCCTACA AGAAGGTGCT CAGGACTCGG TATATACAGA CAGAGCTGGG CTCTCGTGAG 
CGCCTGCTGG TGGCTGTCCT GACTTCTCGA GCCACACTGT CCACCCTGGC CGTCGCGGTT 
AACCGTACAG TAGCACATCA CTTCCCTCGG TTACTCTACT TCACAGGGCA GCGAGGGGCC 
CGGGCTCCTG CAGGGATGCA GGTGGTGTCT CATGGAGATG AGCGGCCTGC CTGGCTCATG 
TCAGAGACCC TGCGCCACCT TCACACACAC TTTGGGGCAG ACTATGACTG GTTCTTCATC 
ATGCAGGACG ACACATATGT GCAGGCCCCC AGATTGGCAG CTCTTGCTGG TCACCTTAGC 
ATCAACCAAG ACCTGTACTT AGGCCATGCA GAGGAATTCA TTGGTACAGG AGAGCAGGCC 
CGGTACTGCC ATGGGGGCTT TGGCTACCTG TTGTCACGGA GTCTCCTATT GCGCTTGCGG 
CCACATCTGG ATGGCTGCCG AGGGGACATT CTCAGTGCCC GGCCTGATGA ATGGCTTGGC 
CGCTGCCTCA TCGACACTCT GGGCATCGGC TGTGTCTCAC AGCACCAGGG GCAACAGTAT 
CGCTCCTTCG AACTGGCCAA GAATAGGGAC CCTGAGAAGG AGGAGAGCCC TGCTTTCCTG 
AGTGCCTTCG CCGTTCACCC GGTCCCCGAG GGCACCCTCA TGTACCGGCT CCACAAACGC 
TTCAGTGCTC TGGAGTTGGA GCGGGCTTAC AGCGAAATAG AACAACTGCA GGCCCAGATC 
CGGAACCTGA CGGTGCTGAC CCCTGAGGGG GAGGCAGGGC TAAGCTGGCC TGTTGGGCTC 
CCGGCCCCTT TCACTCCACA CTCTCGCTTC GAGGTGCTGG GCTGGGACTA CTTCACGGAG 
CAACACACCT TCGCCTGTGC AGACGGGGCT CCCAAGTGTC CCCTGCAGGG GGCTAGCAGG 
GCAGATGTAG GGGACGCTGT GGACACAGCT CTGGAGCAGC TCAATCGGCG CTATCAGCCC 
CGCCTGCGCT TCCGGAAGCA GCGGCTTCTC AATGGCTACC GGCGCTTTGA CCCTGCAAGG 
GGCATGGAAT ACACCCTCGA CTTGCTGCTG GAAGCTGTGA CTCAGCGAGG GCACAGACGC 
TCCCTGGCCC GCAGAGTGAG CCTGCTGAGG CCACTGAGCA GAGTGGAAAT CCTGCCTATG 
CCCTATGTCA CCGAGGCTAC CCGGGTGCAA CTGGTGCTGC CACTCCTGGT GGCTGAAGCT 
CCGGCCGCCT TGGCTTTTCT TGAGGCCTTT GCTGCCAGTG TTCTGGAGCC TCGAGAACAT 
GCATTGCTCA CCCTGTTGTT GGTCTATGGG CCCCGGGAAG GCCGAGGGGG CCCTGACCCA 
TTTCTCGGAG TGAAGGCTGC AGCAGCTGAG TTAGAACGAC GGTACCCAGG GGCGAGGCTG 
GCCTGGCTCG CTGTGCGAGC AGAGGCCCCT TCGCAGGTGC GACTCATGGA TGTCATCTCC 
AAGAAACACC CTGTGGACAC ACTCTTCTTC CTTACCACCG TGTGGACAAG ACCTGGGTCG 
GAAGTCCTCA ACCGCTGCCG TATGAATGCA ATCTCGGGCT GGCAGGCCTT CTTTCCGGTC 
CACTTTCAGG AGTTCAACCC TGCTCTGTCA CCACAGAGAT CTCCCCCAGG TGTCCCTGGG 
GCTGATCCCC CCTCCCCTGG TGTGGACCCT TCTAGGGGGG CTCCGGTCGG GGGCAGATTT 
GACCGGCAGG CTTCAGCAGA GGGCTGCTTC TACAATGCTG ACTACCTGGC AGCCCGCGCC 
CGGCTGGCTG GCGAACTGGC AGGCCAGGAA GAGGAGGAAG CCCTGGAGGG GCTGGAGGTG 
ATGGATGTTT TCCTCCGGTT CTCAGGGCTC CACCTCTTTC GAGCCGTAGA GCCAGGGCTG 
GTGCAGAAGT TCTCTCTGCG GGACTGTAGC CCCCGGCTCA GCGAGGAACT GTACCACCGT 
TGTCGCCTCA GCAATCTGGA GGGGCTGGGG GCCCGTACCC AGCTAGCCAT GGCTCTGTTT 
GAACAGGAAC AGGCCAACAG CACTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

miRBase: integrating microRNA annotation and deep-sequencing data.
Nucleic acids research39(Database issue)D152-7(2011 Jan)
Kozomara A,Griffiths-Jones S

A mouse knockout library for secreted and transmembrane proteins.
Nature biotechnology28(7)749-55(2010 Jul)
Tang T,Li L,Tang J,Li Y,Lin WY,Martin F,Grant D,Solloway M,Parker L,Ye W,Forrest W,Ghilardi N,Oravecz T,Platt KA,Rice DS,Hansen GM,Abuin A,Eberhart DE,Godowski P,Holt KH,Peterson A,Zambrowicz BP,de Sauvage FJ

Prediction of the coding sequences of mouse homologues of KIAA gene: II. The complete nucleotide sequences of 400 mouse KIAA-homologous cDNAs identified by screening of terminal sequences of cDNA clones randomly sampled from size-fractionated libraries.
DNA research : an international journal for rapid publication of reports on genes and genomes10(1)35-48(2003 Feb)
Okazaki N,Kikuno R,Ohara R,Inamoto S,Aizawa H,Yuasa S,Nakajima D,Nagase T,Ohara O,Koga H