Nhsl2 cDNA ORF clone, Mus musculus(house mouse)

The following Nhsl2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Nhsl2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OMu56083 XM_006527724.4
Latest version!
Mus musculus NHS-like 2 (Nhsl2), transcript variant X12, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $475.30
$679.00
OMu56084 XM_006527725.4
Latest version!
Mus musculus NHS-like 2 (Nhsl2), transcript variant X13, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $475.30
$679.00
OMu56085 XM_006527726.4
Latest version!
Mus musculus NHS-like 2 (Nhsl2), transcript variant X14, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $475.30
$679.00
OMu56086 XM_006527727.4
Latest version!
Mus musculus NHS-like 2 (Nhsl2), transcript variant X15, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $475.30
$679.00
OMu56087 XM_006527728.4
Latest version!
Mus musculus NHS-like 2 (Nhsl2), transcript variant X16, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $475.30
$679.00
OMu56088 XM_006527729.4
Latest version!
Mus musculus NHS-like 2 (Nhsl2), transcript variant X18, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $475.30
$679.00
OMu56088 XM_030251165.1
Latest version!
Mus musculus NHS-like 2 (Nhsl2), transcript variant X17, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $475.30
$679.00
OMu56076 XM_030251161.1
Latest version!
Mus musculus NHS-like 2 (Nhsl2), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OMu56077 XM_006527718.4
Latest version!
Mus musculus NHS-like 2 (Nhsl2), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
OMu56078 XM_030251162.1
Latest version!
Mus musculus NHS-like 2 (Nhsl2), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
OMu56079 XM_006527720.4
Latest version!
Mus musculus NHS-like 2 (Nhsl2), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
OMu56080 XM_006527721.4
Latest version!
Mus musculus NHS-like 2 (Nhsl2), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
OMu56081 XM_030251163.1
Latest version!
Mus musculus NHS-like 2 (Nhsl2), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
OMu56082 XM_006527723.4
Latest version!
Mus musculus NHS-like 2 (Nhsl2), transcript variant X11, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
OMu75773 XM_011247499.2
Latest version!
Mus musculus NHS-like 2 (Nhsl2), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OMu75774 XM_011247501.3
Latest version!
Mus musculus NHS-like 2 (Nhsl2), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
OMu75775 XM_011247502.3
Latest version!
Mus musculus NHS-like 2 (Nhsl2), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
OMu83043 XM_017318335.1
Latest version!
Mus musculus NHS-like 2 (Nhsl2), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
OMu83044 XM_017318336.1
Latest version!
Mus musculus NHS-like 2 (Nhsl2), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
OMu90352 XM_030251164.1
Latest version!
Mus musculus NHS-like 2 (Nhsl2), transcript variant X10, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
OMu13929 NM_001163610.1
Latest version!
Mus musculus NHS-like 2 (Nhsl2), mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $643.30
$919.00
View Old Accession Versions >>
OMu56079 XM_006527720.3 Mus musculus NHS-like 2 (Nhsl2), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $559.30
$799.00
OMu75773 XM_011247499.1 Mus musculus NHS-like 2 (Nhsl2), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $643.30
$919.00
OMu75774 XM_011247501.2 Mus musculus NHS-like 2 (Nhsl2), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $559.30
$799.00
OMu56088 XM_006527729.3 Mus musculus NHS-like 2 (Nhsl2), transcript variant X15, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $475.30
$679.00
OMu75775 XM_011247502.2 Mus musculus NHS-like 2 (Nhsl2), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $559.30
$799.00
OMu56086 XM_006527727.3 Mus musculus NHS-like 2 (Nhsl2), transcript variant X13, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $475.30
$679.00
OMu56077 XM_006527718.3 Mus musculus NHS-like 2 (Nhsl2), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $559.30
$799.00
OMu56082 XM_006527723.3 Mus musculus NHS-like 2 (Nhsl2), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $559.30
$799.00
OMu56083 XM_006527724.3 Mus musculus NHS-like 2 (Nhsl2), transcript variant X10, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $475.30
$679.00
OMu56085 XM_006527726.3 Mus musculus NHS-like 2 (Nhsl2), transcript variant X12, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $475.30
$679.00
OMu56080 XM_006527721.3 Mus musculus NHS-like 2 (Nhsl2), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $559.30
$799.00
OMu56084 XM_006527725.3 Mus musculus NHS-like 2 (Nhsl2), transcript variant X11, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $475.30
$679.00
OMu56087 XM_006527728.3 Mus musculus NHS-like 2 (Nhsl2), transcript variant X14, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $475.30
$679.00
Hide Old Accession Versions >>

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone 30% OFF
2-4 Clone 40% OFF
5 or more Clone 50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OMu56083
    Clone ID Related Accession (Same CDS sequence) XM_006527724.4 , XM_006527724.3
    Accession Version XM_006527724.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2973bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-21
    Organism Mus musculus(house mouse)
    Product NHS-like protein 2 isoform X10
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000086.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006527724.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGATGGGCA ACTCTCACCA CAAGCAACCG AGGAGTAAGA GCCAAAGCAG CAGGATGCAT 
    TCAGCAACAG GGCAGCAGTT TGATAGACAT GCAAGTTTTC GACACTCGTT GTTTAACACA
    GAGACAGCCG TGAACCCCAA GTCCACCCTG AGGCGGAGGC GGACCATTAT TGGATTCTCT
    AACTTTTCTC AGCGAGACCA AGGTCACAGC AGCAGCCCCA CAGGCAGCCT GGCCCGCTCT
    GCCACCTCAG ACATCAGGCC TGGCCACTCA GCTCCACAAG TTGTTCAGGG AAGAGTTGCT
    GTTGGGCAGG AAGCTCGGTT CCCAAGTCTC ACCTCACCAG GACTGAGACA CTCTTCTAGT
    GAGCCCGGAG ACGCTCACCA GGCACGCAGT GCCTCAGACC ATCCCGGCAT GGAGAGCATG
    GCAGTGGTGT ACAGTGTCCC CAGTTCTTGC AATGGACCAA CAGAGTCAAC ATTCTCCACT
    TCCTGGAAGG GAGATGCTTT CACATACATG ACCCCAAGTG CCAGCAGCCA GGGCAATCAA
    GTCAGTGAAA ATGGAAAAAA CCCTTCCTCG GGCAATTCTT GGGTCCCTCT GAACACACTC
    CCACCTCTGG TTCCTAAGGA GGCGGCCACG CTCTTTGTCA CCCGTGATAA CCCAGCAGGA
    TGCACTGGGC TACCCAGCTA CTCTGAGCAC CCCACTCTAC GAAGACAGAT ACCAGAAAGA
    CCTCCCAAGA TTGGCCTTCT TGCCCGTGGT ACCTCAAGGC TGGAAACAGG CCCAGGTGGG
    ACCAACAGGT TCCGGGAGCG GTCACTGTCT GTACCCACAG ACTCGGGTGT TACCTCAGTG
    GACTATGACG AAGAACAGAA GACCAGTGAG ACCCGCATCC TGCCTTATGC CAGTACAAGC
    TCCGAGGGCA GTAACAGTAC TGACAACATT GCAGCCCTCA GCACTGAGCA GGAGGCACGG
    CACAGAAGGC AAAGATCCAA AAGTATTTCA CTCAAGAAGG CCAAAAAGAA GCCCTCTCCA
    CCCATGCGAA GTGTCTCACT CGTCAAAGAT GAGCCAGCCC TCCCACCAGA AGGCGAATTG
    GTACTGCCCA AGGACCAGAG GCCTAGGAGC CTTTGCCTCT CCTTGGAACA CCAAGGGCAC
    CACCCACCTC ACCCAGATGC TCAAGGTCAC CCAGCTGTGC CAATGCTCAA AGATCCAGGA
    AGTACGCAGT TCTCTCACCA CTGGTATCTT ACTGACTGGA AGTCTGGTGA CACCTACCAG
    TCCTTGTCCA GCTCCAGCAC TGCCACCGGC ACCACGGTCA TTGAGTGCAC TCAAGTTCAG
    GGAAGCTCAG AGTCTCTGGC CTCCCCTTCC ACCTCCAGAG CGACAACACC CTCCCAGCTC
    TCCATTGAGG TGGAAGCCAG GGAAGTAGCC TCTCCTGGGA GGCCCACTGG ACTGATGTCC
    CCTTCCAGTG GTTACTCCAG CCAGTCAGAG ACACCCACAC CCACTGTCTC CATGTCCTTG
    ACCCTGGGCC ACTTACCTCC TCCAAGTGCT AGTGTCAGAG TACGTCCAGT GGTACCAGAA
    AGGAAGTCAT CACTGCCCCC AACATCACCA ATGGAGAAAA TCTGCAAGTC ACGGTTATCA
    TTTGACCTAC CACTGACCTC TTCAACCACC CTGGATCTGT CGGGGATGAG TATCTCCATC
    CGCAGCAAAA CCAAGGTGAG CCGCCATCAC TCTGATACCA ATTTTGGGGT CAAACTGGCA
    CAGAAAACTA GTCCAAACCA ACCGATCATG CCCATGGTTA CTCAGTCTGA CCTCCGTTCT
    GTTCGCCTGA GGTCAGTCAG CAAGTCTGAG CCAGAAGACG ACATTGAGAG TCCAGATTAC
    ATAGAGGAAC CTGGAGCAGA GGAAGTCTTC ACCATGCCAG AGAGAAAAGT GAAACCTCCC
    ATAGCCGAGA AACCTCCATT GGCCCGAAGG CCTCCAAGTT TGGTCCACAG GCCGCCCTCT
    CTCCCTGGGG AGTATCCACT AACTTCTCCT ACTATGGCTA TGGCATCCAG GAGCTCCATT
    CCACACATGA AGCAGCTTCC CCAAGACAGC TACACAGTGT TGCGGAAACC AAAGTCACCC
    AGCTTCCCCG GGGAGTCAAC AGCATCCTCC AGCCTTGTCC TCTCACCTCT TGCCAGTTCC
    TCTGGTGCTT TCTTCTCAGG AACACAGCAA CCTCCCCAGG CCAGTGTAGA AGACGGGGGC
    CCCAAGGTGA GAGCCCTGCC TGAAAGAATC GGCCTCCAGA GCCAGGAAGA AGCTGAGAAA
    AAGATGACCA AGATTCCACC TCCGGTACCA AAAAAGCCCA GTGTGCTCTA CCTGCCTCTC
    ACCTCCCCTG TAGCTCAAAT GGATGCCTGC ATGGCAGAAC CAAGGCTACC TTTCAGCCCC
    ATCATCACCC TGGAGGAAGA TGGCAAGTGT CCCTCCACTG GTGATGACCA GAAGTCACCT
    GGTAAAGGGG TGACTTCGCC TCTTCACACT GACACAGAAA AAGAGGCCAT CTCTCCAGGG
    AGGTCAGTGG AACCGAGTGC TGAAGAGAAA AGTTTAATCA GTGATAAAAC AGCCGAATGG
    ATTGCAGAGG AGGAGGATGA CGTGTTTGTG GCTTCCCGCA CAACTGAAGA TTTATTTACT
    GTGATACACA GGTCCAAAAG AAAGCTACTG GGCTGGAAAG AGACTGGGGA GGGCTTCACG
    GGAAGCAAAC CCAGCTCCCA TTCACCAGTG AAGAATACAG CTGATTCTCC CACCGGTGAG
    GCTGCTGCTG CCCCAGGGCC AAGTAGCAGT GCCTGCCTAG ATGCTGGTAG AAATGATGAT
    TTCAAGGCCT TGCTCCAGAA GAAGGGAAGT AAGGCAACTC CCAGGACCCG CCCCTCAGCG
    GCCGAGCTGC TGAAGACCAC TAACCCTCTG GCTCGAAGGA TTATTGCACA GTTCTCAAAA
    GACTACGAAC CCACTGACAA CCCCAGTACC TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006527787.1
    CDS275..3247
    Translation

    Target ORF information:

    RefSeq Version XM_006527724.3
    Organism Mus musculus(house mouse)
    Definition Mus musculus NHS-like 2 (Nhsl2), transcript variant X10, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006527724.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGATGGGCA ACTCTCACCA CAAGCAACCG AGGAGTAAGA GCCAAAGCAG CAGGATGCAT 
    TCAGCAACAG GGCAGCAGTT TGATAGACAT GCAAGTTTTC GACACTCGTT GTTTAACACA
    GAGACAGCCG TGAACCCCAA GTCCACCCTG AGGCGGAGGC GGACCATTAT TGGATTCTCT
    AACTTTTCTC AGCGAGACCA AGGTCACAGC AGCAGCCCCA CAGGCAGCCT GGCCCGCTCT
    GCCACCTCAG ACATCAGGCC TGGCCACTCA GCTCCACAAG TTGTTCAGGG AAGAGTTGCT
    GTTGGGCAGG AAGCTCGGTT CCCAAGTCTC ACCTCACCAG GACTGAGACA CTCTTCTAGT
    GAGCCCGGAG ACGCTCACCA GGCACGCAGT GCCTCAGACC ATCCCGGCAT GGAGAGCATG
    GCAGTGGTGT ACAGTGTCCC CAGTTCTTGC AATGGACCAA CAGAGTCAAC ATTCTCCACT
    TCCTGGAAGG GAGATGCTTT CACATACATG ACCCCAAGTG CCAGCAGCCA GGGCAATCAA
    GTCAGTGAAA ATGGAAAAAA CCCTTCCTCG GGCAATTCTT GGGTCCCTCT GAACACACTC
    CCACCTCTGG TTCCTAAGGA GGCGGCCACG CTCTTTGTCA CCCGTGATAA CCCAGCAGGA
    TGCACTGGGC TACCCAGCTA CTCTGAGCAC CCCACTCTAC GAAGACAGAT ACCAGAAAGA
    CCTCCCAAGA TTGGCCTTCT TGCCCGTGGT ACCTCAAGGC TGGAAACAGG CCCAGGTGGG
    ACCAACAGGT TCCGGGAGCG GTCACTGTCT GTACCCACAG ACTCGGGTGT TACCTCAGTG
    GACTATGACG AAGAACAGAA GACCAGTGAG ACCCGCATCC TGCCTTATGC CAGTACAAGC
    TCCGAGGGCA GTAACAGTAC TGACAACATT GCAGCCCTCA GCACTGAGCA GGAGGCACGG
    CACAGAAGGC AAAGATCCAA AAGTATTTCA CTCAAGAAGG CCAAAAAGAA GCCCTCTCCA
    CCCATGCGAA GTGTCTCACT CGTCAAAGAT GAGCCAGCCC TCCCACCAGA AGGCGAATTG
    GTACTGCCCA AGGACCAGAG GCCTAGGAGC CTTTGCCTCT CCTTGGAACA CCAAGGGCAC
    CACCCACCTC ACCCAGATGC TCAAGGTCAC CCAGCTGTGC CAATGCTCAA AGATCCAGGA
    AGTACGCAGT TCTCTCACCA CTGGTATCTT ACTGACTGGA AGTCTGGTGA CACCTACCAG
    TCCTTGTCCA GCTCCAGCAC TGCCACCGGC ACCACGGTCA TTGAGTGCAC TCAAGTTCAG
    GGAAGCTCAG AGTCTCTGGC CTCCCCTTCC ACCTCCAGAG CGACAACACC CTCCCAGCTC
    TCCATTGAGG TGGAAGCCAG GGAAGTAGCC TCTCCTGGGA GGCCCACTGG ACTGATGTCC
    CCTTCCAGTG GTTACTCCAG CCAGTCAGAG ACACCCACAC CCACTGTCTC CATGTCCTTG
    ACCCTGGGCC ACTTACCTCC TCCAAGTGCT AGTGTCAGAG TACGTCCAGT GGTACCAGAA
    AGGAAGTCAT CACTGCCCCC AACATCACCA ATGGAGAAAA TCTGCAAGTC ACGGTTATCA
    TTTGACCTAC CACTGACCTC TTCAACCACC CTGGATCTGT CGGGGATGAG TATCTCCATC
    CGCAGCAAAA CCAAGGTGAG CCGCCATCAC TCTGATACCA ATTTTGGGGT CAAACTGGCA
    CAGAAAACTA GTCCAAACCA ACCGATCATG CCCATGGTTA CTCAGTCTGA CCTCCGTTCT
    GTTCGCCTGA GGTCAGTCAG CAAGTCTGAG CCAGAAGACG ACATTGAGAG TCCAGATTAC
    ATAGAGGAAC CTGGAGCAGA GGAAGTCTTC ACCATGCCAG AGAGAAAAGT GAAACCTCCC
    ATAGCCGAGA AACCTCCATT GGCCCGAAGG CCTCCAAGTT TGGTCCACAG GCCGCCCTCT
    CTCCCTGGGG AGTATCCACT AACTTCTCCT ACTATGGCTA TGGCATCCAG GAGCTCCATT
    CCACACATGA AGCAGCTTCC CCAAGACAGC TACACAGTGT TGCGGAAACC AAAGTCACCC
    AGCTTCCCCG GGGAGTCAAC AGCATCCTCC AGCCTTGTCC TCTCACCTCT TGCCAGTTCC
    TCTGGTGCTT TCTTCTCAGG AACACAGCAA CCTCCCCAGG CCAGTGTAGA AGACGGGGGC
    CCCAAGGTGA GAGCCCTGCC TGAAAGAATC GGCCTCCAGA GCCAGGAAGA AGCTGAGAAA
    AAGATGACCA AGATTCCACC TCCGGTACCA AAAAAGCCCA GTGTGCTCTA CCTGCCTCTC
    ACCTCCCCTG TAGCTCAAAT GGATGCCTGC ATGGCAGAAC CAAGGCTACC TTTCAGCCCC
    ATCATCACCC TGGAGGAAGA TGGCAAGTGT CCCTCCACTG GTGATGACCA GAAGTCACCT
    GGTAAAGGGG TGACTTCGCC TCTTCACACT GACACAGAAA AAGAGGCCAT CTCTCCAGGG
    AGGTCAGTGG AACCGAGTGC TGAAGAGAAA AGTTTAATCA GTGATAAAAC AGCCGAATGG
    ATTGCAGAGG AGGAGGATGA CGTGTTTGTG GCTTCCCGCA CAACTGAAGA TTTATTTACT
    GTGATACACA GGTCCAAAAG AAAGCTACTG GGCTGGAAAG AGACTGGGGA GGGCTTCACG
    GGAAGCAAAC CCAGCTCCCA TTCACCAGTG AAGAATACAG CTGATTCTCC CACCGGTGAG
    GCTGCTGCTG CCCCAGGGCC AAGTAGCAGT GCCTGCCTAG ATGCTGGTAG AAATGATGAT
    TTCAAGGCCT TGCTCCAGAA GAAGGGAAGT AAGGCAACTC CCAGGACCCG CCCCTCAGCG
    GCCGAGCTGC TGAAGACCAC TAACCCTCTG GCTCGAAGGA TTATTGCACA GTTCTCAAAA
    GACTACGAAC CCACTGACAA CCCCAGTACC TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu56084
    Clone ID Related Accession (Same CDS sequence) XM_006527725.3 , XM_006527725.4
    Accession Version XM_006527725.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2946bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-21
    Organism Mus musculus(house mouse)
    Product NHS-like protein 2 isoform X11
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000086.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006527725.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGGAGAGAG GCGAGTCTCT TACCCTCTTC TGGAGCCGGG GAGGGCAGCA GTTTGATAGA 
    CATGCAAGTT TTCGACACTC GTTGTTTAAC ACAGAGACAG CCGTGAACCC CAAGTCCACC
    CTGAGGCGGA GGCGGACCAT TATTGGATTC TCTAACTTTT CTCAGCGAGA CCAAGGTCAC
    AGCAGCAGCC CCACAGGCAG CCTGGCCCGC TCTGCCACCT CAGACATCAG GCCTGGCCAC
    TCAGCTCCAC AAGTTGTTCA GGGAAGAGTT GCTGTTGGGC AGGAAGCTCG GTTCCCAAGT
    CTCACCTCAC CAGGACTGAG ACACTCTTCT AGTGAGCCCG GAGACGCTCA CCAGGCACGC
    AGTGCCTCAG ACCATCCCGG CATGGAGAGC ATGGCAGTGG TGTACAGTGT CCCCAGTTCT
    TGCAATGGAC CAACAGAGTC AACATTCTCC ACTTCCTGGA AGGGAGATGC TTTCACATAC
    ATGACCCCAA GTGCCAGCAG CCAGGGCAAT CAAGTCAGTG AAAATGGAAA AAACCCTTCC
    TCGGGCAATT CTTGGGTCCC TCTGAACACA CTCCCACCTC TGGTTCCTAA GGAGGCGGCC
    ACGCTCTTTG TCACCCGTGA TAACCCAGCA GGATGCACTG GGCTACCCAG CTACTCTGAG
    CACCCCACTC TACGAAGACA GATACCAGAA AGACCTCCCA AGATTGGCCT TCTTGCCCGT
    GGTACCTCAA GGCTGGAAAC AGGCCCAGGT GGGACCAACA GGTTCCGGGA GCGGTCACTG
    TCTGTACCCA CAGACTCGGG TGTTACCTCA GTGGACTATG ACGAAGAACA GAAGACCAGT
    GAGACCCGCA TCCTGCCTTA TGCCAGTACA AGCTCCGAGG GCAGTAACAG TACTGACAAC
    ATTGCAGCCC TCAGCACTGA GCAGGAGGCA CGGCACAGAA GGCAAAGATC CAAAAGTATT
    TCACTCAAGA AGGCCAAAAA GAAGCCCTCT CCACCCATGC GAAGTGTCTC ACTCGTCAAA
    GATGAGCCAG CCCTCCCACC AGAAGGCGAA TTGGTACTGC CCAAGGACCA GAGGCCTAGG
    AGCCTTTGCC TCTCCTTGGA ACACCAAGGG CACCACCCAC CTCACCCAGA TGCTCAAGGT
    CACCCAGCTG TGCCAATGCT CAAAGATCCA GGAAGTACGC AGTTCTCTCA CCACTGGTAT
    CTTACTGACT GGAAGTCTGG TGACACCTAC CAGTCCTTGT CCAGCTCCAG CACTGCCACC
    GGCACCACGG TCATTGAGTG CACTCAAGTT CAGGGAAGCT CAGAGTCTCT GGCCTCCCCT
    TCCACCTCCA GAGCGACAAC ACCCTCCCAG CTCTCCATTG AGGTGGAAGC CAGGGAAGTA
    GCCTCTCCTG GGAGGCCCAC TGGACTGATG TCCCCTTCCA GTGGTTACTC CAGCCAGTCA
    GAGACACCCA CACCCACTGT CTCCATGTCC TTGACCCTGG GCCACTTACC TCCTCCAAGT
    GCTAGTGTCA GAGTACGTCC AGTGGTACCA GAAAGGAAGT CATCACTGCC CCCAACATCA
    CCAATGGAGA AAATCTGCAA GTCACGGTTA TCATTTGACC TACCACTGAC CTCTTCAACC
    ACCCTGGATC TGTCGGGGAT GAGTATCTCC ATCCGCAGCA AAACCAAGGT GAGCCGCCAT
    CACTCTGATA CCAATTTTGG GGTCAAACTG GCACAGAAAA CTAGTCCAAA CCAACCGATC
    ATGCCCATGG TTACTCAGTC TGACCTCCGT TCTGTTCGCC TGAGGTCAGT CAGCAAGTCT
    GAGCCAGAAG ACGACATTGA GAGTCCAGAT TACATAGAGG AACCTGGAGC AGAGGAAGTC
    TTCACCATGC CAGAGAGAAA AGTGAAACCT CCCATAGCCG AGAAACCTCC ATTGGCCCGA
    AGGCCTCCAA GTTTGGTCCA CAGGCCGCCC TCTCTCCCTG GGGAGTATCC ACTAACTTCT
    CCTACTATGG CTATGGCATC CAGGAGCTCC ATTCCACACA TGAAGCAGCT TCCCCAAGAC
    AGCTACACAG TGTTGCGGAA ACCAAAGTCA CCCAGCTTCC CCGGGGAGTC AACAGCATCC
    TCCAGCCTTG TCCTCTCACC TCTTGCCAGT TCCTCTGGTG CTTTCTTCTC AGGAACACAG
    CAACCTCCCC AGGCCAGTGT AGAAGACGGG GGCCCCAAGG TGAGAGCCCT GCCTGAAAGA
    ATCGGCCTCC AGAGCCAGGA AGAAGCTGAG AAAAAGATGA CCAAGATTCC ACCTCCGGTA
    CCAAAAAAGC CCAGTGTGCT CTACCTGCCT CTCACCTCCC CTGTAGCTCA AATGGATGCC
    TGCATGGCAG AACCAAGGCT ACCTTTCAGC CCCATCATCA CCCTGGAGGA AGATGGCAAG
    TGTCCCTCCA CTGGTGATGA CCAGAAGTCA CCTGGTAAAG GGGTGACTTC GCCTCTTCAC
    ACTGACACAG AAAAAGAGGC CATCTCTCCA GGGAGGTCAG TGGAACCGAG TGCTGAAGAG
    AAAAGTTTAA TCAGTGATAA AACAGCCGAA TGGATTGCAG AGGAGGAGGA TGACGTGTTT
    GTGGCTTCCC GCACAACTGA AGATTTATTT ACTGTGATAC ACAGGTCCAA AAGAAAGCTA
    CTGGGCTGGA AAGAGACTGG GGAGGGCTTC ACGGGAAGCA AACCCAGCTC CCATTCACCA
    GTGAAGAATA CAGCTGATTC TCCCACCGGT GAGGCTGCTG CTGCCCCAGG GCCAAGTAGC
    AGTGCCTGCC TAGATGCTGG TAGAAATGAT GATTTCAAGG CCTTGCTCCA GAAGAAGGGA
    AGTAAGGCAA CTCCCAGGAC CCGCCCCTCA GCGGCCGAGC TGCTGAAGAC CACTAACCCT
    CTGGCTCGAA GGATTATTGC ACAGTTCTCA AAAGACTACG AACCCACTGA CAACCCCAGT
    ACCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006527788.1
    CDS1915..4860
    Translation

    Target ORF information:

    RefSeq Version XM_006527725.3
    Organism Mus musculus(house mouse)
    Definition Mus musculus NHS-like 2 (Nhsl2), transcript variant X11, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006527725.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGGAGAGAG GCGAGTCTCT TACCCTCTTC TGGAGCCGGG GAGGGCAGCA GTTTGATAGA 
    CATGCAAGTT TTCGACACTC GTTGTTTAAC ACAGAGACAG CCGTGAACCC CAAGTCCACC
    CTGAGGCGGA GGCGGACCAT TATTGGATTC TCTAACTTTT CTCAGCGAGA CCAAGGTCAC
    AGCAGCAGCC CCACAGGCAG CCTGGCCCGC TCTGCCACCT CAGACATCAG GCCTGGCCAC
    TCAGCTCCAC AAGTTGTTCA GGGAAGAGTT GCTGTTGGGC AGGAAGCTCG GTTCCCAAGT
    CTCACCTCAC CAGGACTGAG ACACTCTTCT AGTGAGCCCG GAGACGCTCA CCAGGCACGC
    AGTGCCTCAG ACCATCCCGG CATGGAGAGC ATGGCAGTGG TGTACAGTGT CCCCAGTTCT
    TGCAATGGAC CAACAGAGTC AACATTCTCC ACTTCCTGGA AGGGAGATGC TTTCACATAC
    ATGACCCCAA GTGCCAGCAG CCAGGGCAAT CAAGTCAGTG AAAATGGAAA AAACCCTTCC
    TCGGGCAATT CTTGGGTCCC TCTGAACACA CTCCCACCTC TGGTTCCTAA GGAGGCGGCC
    ACGCTCTTTG TCACCCGTGA TAACCCAGCA GGATGCACTG GGCTACCCAG CTACTCTGAG
    CACCCCACTC TACGAAGACA GATACCAGAA AGACCTCCCA AGATTGGCCT TCTTGCCCGT
    GGTACCTCAA GGCTGGAAAC AGGCCCAGGT GGGACCAACA GGTTCCGGGA GCGGTCACTG
    TCTGTACCCA CAGACTCGGG TGTTACCTCA GTGGACTATG ACGAAGAACA GAAGACCAGT
    GAGACCCGCA TCCTGCCTTA TGCCAGTACA AGCTCCGAGG GCAGTAACAG TACTGACAAC
    ATTGCAGCCC TCAGCACTGA GCAGGAGGCA CGGCACAGAA GGCAAAGATC CAAAAGTATT
    TCACTCAAGA AGGCCAAAAA GAAGCCCTCT CCACCCATGC GAAGTGTCTC ACTCGTCAAA
    GATGAGCCAG CCCTCCCACC AGAAGGCGAA TTGGTACTGC CCAAGGACCA GAGGCCTAGG
    AGCCTTTGCC TCTCCTTGGA ACACCAAGGG CACCACCCAC CTCACCCAGA TGCTCAAGGT
    CACCCAGCTG TGCCAATGCT CAAAGATCCA GGAAGTACGC AGTTCTCTCA CCACTGGTAT
    CTTACTGACT GGAAGTCTGG TGACACCTAC CAGTCCTTGT CCAGCTCCAG CACTGCCACC
    GGCACCACGG TCATTGAGTG CACTCAAGTT CAGGGAAGCT CAGAGTCTCT GGCCTCCCCT
    TCCACCTCCA GAGCGACAAC ACCCTCCCAG CTCTCCATTG AGGTGGAAGC CAGGGAAGTA
    GCCTCTCCTG GGAGGCCCAC TGGACTGATG TCCCCTTCCA GTGGTTACTC CAGCCAGTCA
    GAGACACCCA CACCCACTGT CTCCATGTCC TTGACCCTGG GCCACTTACC TCCTCCAAGT
    GCTAGTGTCA GAGTACGTCC AGTGGTACCA GAAAGGAAGT CATCACTGCC CCCAACATCA
    CCAATGGAGA AAATCTGCAA GTCACGGTTA TCATTTGACC TACCACTGAC CTCTTCAACC
    ACCCTGGATC TGTCGGGGAT GAGTATCTCC ATCCGCAGCA AAACCAAGGT GAGCCGCCAT
    CACTCTGATA CCAATTTTGG GGTCAAACTG GCACAGAAAA CTAGTCCAAA CCAACCGATC
    ATGCCCATGG TTACTCAGTC TGACCTCCGT TCTGTTCGCC TGAGGTCAGT CAGCAAGTCT
    GAGCCAGAAG ACGACATTGA GAGTCCAGAT TACATAGAGG AACCTGGAGC AGAGGAAGTC
    TTCACCATGC CAGAGAGAAA AGTGAAACCT CCCATAGCCG AGAAACCTCC ATTGGCCCGA
    AGGCCTCCAA GTTTGGTCCA CAGGCCGCCC TCTCTCCCTG GGGAGTATCC ACTAACTTCT
    CCTACTATGG CTATGGCATC CAGGAGCTCC ATTCCACACA TGAAGCAGCT TCCCCAAGAC
    AGCTACACAG TGTTGCGGAA ACCAAAGTCA CCCAGCTTCC CCGGGGAGTC AACAGCATCC
    TCCAGCCTTG TCCTCTCACC TCTTGCCAGT TCCTCTGGTG CTTTCTTCTC AGGAACACAG
    CAACCTCCCC AGGCCAGTGT AGAAGACGGG GGCCCCAAGG TGAGAGCCCT GCCTGAAAGA
    ATCGGCCTCC AGAGCCAGGA AGAAGCTGAG AAAAAGATGA CCAAGATTCC ACCTCCGGTA
    CCAAAAAAGC CCAGTGTGCT CTACCTGCCT CTCACCTCCC CTGTAGCTCA AATGGATGCC
    TGCATGGCAG AACCAAGGCT ACCTTTCAGC CCCATCATCA CCCTGGAGGA AGATGGCAAG
    TGTCCCTCCA CTGGTGATGA CCAGAAGTCA CCTGGTAAAG GGGTGACTTC GCCTCTTCAC
    ACTGACACAG AAAAAGAGGC CATCTCTCCA GGGAGGTCAG TGGAACCGAG TGCTGAAGAG
    AAAAGTTTAA TCAGTGATAA AACAGCCGAA TGGATTGCAG AGGAGGAGGA TGACGTGTTT
    GTGGCTTCCC GCACAACTGA AGATTTATTT ACTGTGATAC ACAGGTCCAA AAGAAAGCTA
    CTGGGCTGGA AAGAGACTGG GGAGGGCTTC ACGGGAAGCA AACCCAGCTC CCATTCACCA
    GTGAAGAATA CAGCTGATTC TCCCACCGGT GAGGCTGCTG CTGCCCCAGG GCCAAGTAGC
    AGTGCCTGCC TAGATGCTGG TAGAAATGAT GATTTCAAGG CCTTGCTCCA GAAGAAGGGA
    AGTAAGGCAA CTCCCAGGAC CCGCCCCTCA GCGGCCGAGC TGCTGAAGAC CACTAACCCT
    CTGGCTCGAA GGATTATTGC ACAGTTCTCA AAAGACTACG AACCCACTGA CAACCCCAGT
    ACCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu56085
    Clone ID Related Accession (Same CDS sequence) XM_006527726.4 , XM_006527726.3
    Accession Version XM_006527726.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2931bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-21
    Organism Mus musculus(house mouse)
    Product NHS-like protein 2 isoform X12
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000086.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006527726.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    ATGGCAGCAC AGTCTGGAGC TTGGAATGGG CAGCAGTTTG ATAGACATGC AAGTTTTCGA 
    CACTCGTTGT TTAACACAGA GACAGCCGTG AACCCCAAGT CCACCCTGAG GCGGAGGCGG
    ACCATTATTG GATTCTCTAA CTTTTCTCAG CGAGACCAAG GTCACAGCAG CAGCCCCACA
    GGCAGCCTGG CCCGCTCTGC CACCTCAGAC ATCAGGCCTG GCCACTCAGC TCCACAAGTT
    GTTCAGGGAA GAGTTGCTGT TGGGCAGGAA GCTCGGTTCC CAAGTCTCAC CTCACCAGGA
    CTGAGACACT CTTCTAGTGA GCCCGGAGAC GCTCACCAGG CACGCAGTGC CTCAGACCAT
    CCCGGCATGG AGAGCATGGC AGTGGTGTAC AGTGTCCCCA GTTCTTGCAA TGGACCAACA
    GAGTCAACAT TCTCCACTTC CTGGAAGGGA GATGCTTTCA CATACATGAC CCCAAGTGCC
    AGCAGCCAGG GCAATCAAGT CAGTGAAAAT GGAAAAAACC CTTCCTCGGG CAATTCTTGG
    GTCCCTCTGA ACACACTCCC ACCTCTGGTT CCTAAGGAGG CGGCCACGCT CTTTGTCACC
    CGTGATAACC CAGCAGGATG CACTGGGCTA CCCAGCTACT CTGAGCACCC CACTCTACGA
    AGACAGATAC CAGAAAGACC TCCCAAGATT GGCCTTCTTG CCCGTGGTAC CTCAAGGCTG
    GAAACAGGCC CAGGTGGGAC CAACAGGTTC CGGGAGCGGT CACTGTCTGT ACCCACAGAC
    TCGGGTGTTA CCTCAGTGGA CTATGACGAA GAACAGAAGA CCAGTGAGAC CCGCATCCTG
    CCTTATGCCA GTACAAGCTC CGAGGGCAGT AACAGTACTG ACAACATTGC AGCCCTCAGC
    ACTGAGCAGG AGGCACGGCA CAGAAGGCAA AGATCCAAAA GTATTTCACT CAAGAAGGCC
    AAAAAGAAGC CCTCTCCACC CATGCGAAGT GTCTCACTCG TCAAAGATGA GCCAGCCCTC
    CCACCAGAAG GCGAATTGGT ACTGCCCAAG GACCAGAGGC CTAGGAGCCT TTGCCTCTCC
    TTGGAACACC AAGGGCACCA CCCACCTCAC CCAGATGCTC AAGGTCACCC AGCTGTGCCA
    ATGCTCAAAG ATCCAGGAAG TACGCAGTTC TCTCACCACT GGTATCTTAC TGACTGGAAG
    TCTGGTGACA CCTACCAGTC CTTGTCCAGC TCCAGCACTG CCACCGGCAC CACGGTCATT
    GAGTGCACTC AAGTTCAGGG AAGCTCAGAG TCTCTGGCCT CCCCTTCCAC CTCCAGAGCG
    ACAACACCCT CCCAGCTCTC CATTGAGGTG GAAGCCAGGG AAGTAGCCTC TCCTGGGAGG
    CCCACTGGAC TGATGTCCCC TTCCAGTGGT TACTCCAGCC AGTCAGAGAC ACCCACACCC
    ACTGTCTCCA TGTCCTTGAC CCTGGGCCAC TTACCTCCTC CAAGTGCTAG TGTCAGAGTA
    CGTCCAGTGG TACCAGAAAG GAAGTCATCA CTGCCCCCAA CATCACCAAT GGAGAAAATC
    TGCAAGTCAC GGTTATCATT TGACCTACCA CTGACCTCTT CAACCACCCT GGATCTGTCG
    GGGATGAGTA TCTCCATCCG CAGCAAAACC AAGGTGAGCC GCCATCACTC TGATACCAAT
    TTTGGGGTCA AACTGGCACA GAAAACTAGT CCAAACCAAC CGATCATGCC CATGGTTACT
    CAGTCTGACC TCCGTTCTGT TCGCCTGAGG TCAGTCAGCA AGTCTGAGCC AGAAGACGAC
    ATTGAGAGTC CAGATTACAT AGAGGAACCT GGAGCAGAGG AAGTCTTCAC CATGCCAGAG
    AGAAAAGTGA AACCTCCCAT AGCCGAGAAA CCTCCATTGG CCCGAAGGCC TCCAAGTTTG
    GTCCACAGGC CGCCCTCTCT CCCTGGGGAG TATCCACTAA CTTCTCCTAC TATGGCTATG
    GCATCCAGGA GCTCCATTCC ACACATGAAG CAGCTTCCCC AAGACAGCTA CACAGTGTTG
    CGGAAACCAA AGTCACCCAG CTTCCCCGGG GAGTCAACAG CATCCTCCAG CCTTGTCCTC
    TCACCTCTTG CCAGTTCCTC TGGTGCTTTC TTCTCAGGAA CACAGCAACC TCCCCAGGCC
    AGTGTAGAAG ACGGGGGCCC CAAGGTGAGA GCCCTGCCTG AAAGAATCGG CCTCCAGAGC
    CAGGAAGAAG CTGAGAAAAA GATGACCAAG ATTCCACCTC CGGTACCAAA AAAGCCCAGT
    GTGCTCTACC TGCCTCTCAC CTCCCCTGTA GCTCAAATGG ATGCCTGCAT GGCAGAACCA
    AGGCTACCTT TCAGCCCCAT CATCACCCTG GAGGAAGATG GCAAGTGTCC CTCCACTGGT
    GATGACCAGA AGTCACCTGG TAAAGGGGTG ACTTCGCCTC TTCACACTGA CACAGAAAAA
    GAGGCCATCT CTCCAGGGAG GTCAGTGGAA CCGAGTGCTG AAGAGAAAAG TTTAATCAGT
    GATAAAACAG CCGAATGGAT TGCAGAGGAG GAGGATGACG TGTTTGTGGC TTCCCGCACA
    ACTGAAGATT TATTTACTGT GATACACAGG TCCAAAAGAA AGCTACTGGG CTGGAAAGAG
    ACTGGGGAGG GCTTCACGGG AAGCAAACCC AGCTCCCATT CACCAGTGAA GAATACAGCT
    GATTCTCCCA CCGGTGAGGC TGCTGCTGCC CCAGGGCCAA GTAGCAGTGC CTGCCTAGAT
    GCTGGTAGAA ATGATGATTT CAAGGCCTTG CTCCAGAAGA AGGGAAGTAA GGCAACTCCC
    AGGACCCGCC CCTCAGCGGC CGAGCTGCTG AAGACCACTA ACCCTCTGGC TCGAAGGATT
    ATTGCACAGT TCTCAAAAGA CTACGAACCC ACTGACAACC CCAGTACCTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006527789.1
    CDS154..3084
    Translation

    Target ORF information:

    RefSeq Version XM_006527726.3
    Organism Mus musculus(house mouse)
    Definition Mus musculus NHS-like 2 (Nhsl2), transcript variant X12, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006527726.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    ATGGCAGCAC AGTCTGGAGC TTGGAATGGG CAGCAGTTTG ATAGACATGC AAGTTTTCGA 
    CACTCGTTGT TTAACACAGA GACAGCCGTG AACCCCAAGT CCACCCTGAG GCGGAGGCGG
    ACCATTATTG GATTCTCTAA CTTTTCTCAG CGAGACCAAG GTCACAGCAG CAGCCCCACA
    GGCAGCCTGG CCCGCTCTGC CACCTCAGAC ATCAGGCCTG GCCACTCAGC TCCACAAGTT
    GTTCAGGGAA GAGTTGCTGT TGGGCAGGAA GCTCGGTTCC CAAGTCTCAC CTCACCAGGA
    CTGAGACACT CTTCTAGTGA GCCCGGAGAC GCTCACCAGG CACGCAGTGC CTCAGACCAT
    CCCGGCATGG AGAGCATGGC AGTGGTGTAC AGTGTCCCCA GTTCTTGCAA TGGACCAACA
    GAGTCAACAT TCTCCACTTC CTGGAAGGGA GATGCTTTCA CATACATGAC CCCAAGTGCC
    AGCAGCCAGG GCAATCAAGT CAGTGAAAAT GGAAAAAACC CTTCCTCGGG CAATTCTTGG
    GTCCCTCTGA ACACACTCCC ACCTCTGGTT CCTAAGGAGG CGGCCACGCT CTTTGTCACC
    CGTGATAACC CAGCAGGATG CACTGGGCTA CCCAGCTACT CTGAGCACCC CACTCTACGA
    AGACAGATAC CAGAAAGACC TCCCAAGATT GGCCTTCTTG CCCGTGGTAC CTCAAGGCTG
    GAAACAGGCC CAGGTGGGAC CAACAGGTTC CGGGAGCGGT CACTGTCTGT ACCCACAGAC
    TCGGGTGTTA CCTCAGTGGA CTATGACGAA GAACAGAAGA CCAGTGAGAC CCGCATCCTG
    CCTTATGCCA GTACAAGCTC CGAGGGCAGT AACAGTACTG ACAACATTGC AGCCCTCAGC
    ACTGAGCAGG AGGCACGGCA CAGAAGGCAA AGATCCAAAA GTATTTCACT CAAGAAGGCC
    AAAAAGAAGC CCTCTCCACC CATGCGAAGT GTCTCACTCG TCAAAGATGA GCCAGCCCTC
    CCACCAGAAG GCGAATTGGT ACTGCCCAAG GACCAGAGGC CTAGGAGCCT TTGCCTCTCC
    TTGGAACACC AAGGGCACCA CCCACCTCAC CCAGATGCTC AAGGTCACCC AGCTGTGCCA
    ATGCTCAAAG ATCCAGGAAG TACGCAGTTC TCTCACCACT GGTATCTTAC TGACTGGAAG
    TCTGGTGACA CCTACCAGTC CTTGTCCAGC TCCAGCACTG CCACCGGCAC CACGGTCATT
    GAGTGCACTC AAGTTCAGGG AAGCTCAGAG TCTCTGGCCT CCCCTTCCAC CTCCAGAGCG
    ACAACACCCT CCCAGCTCTC CATTGAGGTG GAAGCCAGGG AAGTAGCCTC TCCTGGGAGG
    CCCACTGGAC TGATGTCCCC TTCCAGTGGT TACTCCAGCC AGTCAGAGAC ACCCACACCC
    ACTGTCTCCA TGTCCTTGAC CCTGGGCCAC TTACCTCCTC CAAGTGCTAG TGTCAGAGTA
    CGTCCAGTGG TACCAGAAAG GAAGTCATCA CTGCCCCCAA CATCACCAAT GGAGAAAATC
    TGCAAGTCAC GGTTATCATT TGACCTACCA CTGACCTCTT CAACCACCCT GGATCTGTCG
    GGGATGAGTA TCTCCATCCG CAGCAAAACC AAGGTGAGCC GCCATCACTC TGATACCAAT
    TTTGGGGTCA AACTGGCACA GAAAACTAGT CCAAACCAAC CGATCATGCC CATGGTTACT
    CAGTCTGACC TCCGTTCTGT TCGCCTGAGG TCAGTCAGCA AGTCTGAGCC AGAAGACGAC
    ATTGAGAGTC CAGATTACAT AGAGGAACCT GGAGCAGAGG AAGTCTTCAC CATGCCAGAG
    AGAAAAGTGA AACCTCCCAT AGCCGAGAAA CCTCCATTGG CCCGAAGGCC TCCAAGTTTG
    GTCCACAGGC CGCCCTCTCT CCCTGGGGAG TATCCACTAA CTTCTCCTAC TATGGCTATG
    GCATCCAGGA GCTCCATTCC ACACATGAAG CAGCTTCCCC AAGACAGCTA CACAGTGTTG
    CGGAAACCAA AGTCACCCAG CTTCCCCGGG GAGTCAACAG CATCCTCCAG CCTTGTCCTC
    TCACCTCTTG CCAGTTCCTC TGGTGCTTTC TTCTCAGGAA CACAGCAACC TCCCCAGGCC
    AGTGTAGAAG ACGGGGGCCC CAAGGTGAGA GCCCTGCCTG AAAGAATCGG CCTCCAGAGC
    CAGGAAGAAG CTGAGAAAAA GATGACCAAG ATTCCACCTC CGGTACCAAA AAAGCCCAGT
    GTGCTCTACC TGCCTCTCAC CTCCCCTGTA GCTCAAATGG ATGCCTGCAT GGCAGAACCA
    AGGCTACCTT TCAGCCCCAT CATCACCCTG GAGGAAGATG GCAAGTGTCC CTCCACTGGT
    GATGACCAGA AGTCACCTGG TAAAGGGGTG ACTTCGCCTC TTCACACTGA CACAGAAAAA
    GAGGCCATCT CTCCAGGGAG GTCAGTGGAA CCGAGTGCTG AAGAGAAAAG TTTAATCAGT
    GATAAAACAG CCGAATGGAT TGCAGAGGAG GAGGATGACG TGTTTGTGGC TTCCCGCACA
    ACTGAAGATT TATTTACTGT GATACACAGG TCCAAAAGAA AGCTACTGGG CTGGAAAGAG
    ACTGGGGAGG GCTTCACGGG AAGCAAACCC AGCTCCCATT CACCAGTGAA GAATACAGCT
    GATTCTCCCA CCGGTGAGGC TGCTGCTGCC CCAGGGCCAA GTAGCAGTGC CTGCCTAGAT
    GCTGGTAGAA ATGATGATTT CAAGGCCTTG CTCCAGAAGA AGGGAAGTAA GGCAACTCCC
    AGGACCCGCC CCTCAGCGGC CGAGCTGCTG AAGACCACTA ACCCTCTGGC TCGAAGGATT
    ATTGCACAGT TCTCAAAAGA CTACGAACCC ACTGACAACC CCAGTACCTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu56086
    Clone ID Related Accession (Same CDS sequence) XM_006527727.3 , XM_006527727.4
    Accession Version XM_006527727.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2841bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-21
    Organism Mus musculus(house mouse)
    Product NHS-like protein 2 isoform X13
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000086.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006527727.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    ATGATGGGCA ACTCTCACCA CAAGCAACCG AGGAGTAAGA GCCAAAGCAG CAGGATGCAT 
    TCAGCAACAG GTCACAGCAG CAGCCCCACA GGCAGCCTGG CCCGCTCTGC CACCTCAGAC
    ATCAGGCCTG GCCACTCAGC TCCACAAGTT GTTCAGGGAA GAGTTGCTGT TGGGCAGGAA
    GCTCGGTTCC CAAGTCTCAC CTCACCAGGA CTGAGACACT CTTCTAGTGA GCCCGGAGAC
    GCTCACCAGG CACGCAGTGC CTCAGACCAT CCCGGCATGG AGAGCATGGC AGTGGTGTAC
    AGTGTCCCCA GTTCTTGCAA TGGACCAACA GAGTCAACAT TCTCCACTTC CTGGAAGGGA
    GATGCTTTCA CATACATGAC CCCAAGTGCC AGCAGCCAGG GCAATCAAGT CAGTGAAAAT
    GGAAAAAACC CTTCCTCGGG CAATTCTTGG GTCCCTCTGA ACACACTCCC ACCTCTGGTT
    CCTAAGGAGG CGGCCACGCT CTTTGTCACC CGTGATAACC CAGCAGGATG CACTGGGCTA
    CCCAGCTACT CTGAGCACCC CACTCTACGA AGACAGATAC CAGAAAGACC TCCCAAGATT
    GGCCTTCTTG CCCGTGGTAC CTCAAGGCTG GAAACAGGCC CAGGTGGGAC CAACAGGTTC
    CGGGAGCGGT CACTGTCTGT ACCCACAGAC TCGGGTGTTA CCTCAGTGGA CTATGACGAA
    GAACAGAAGA CCAGTGAGAC CCGCATCCTG CCTTATGCCA GTACAAGCTC CGAGGGCAGT
    AACAGTACTG ACAACATTGC AGCCCTCAGC ACTGAGCAGG AGGCACGGCA CAGAAGGCAA
    AGATCCAAAA GTATTTCACT CAAGAAGGCC AAAAAGAAGC CCTCTCCACC CATGCGAAGT
    GTCTCACTCG TCAAAGATGA GCCAGCCCTC CCACCAGAAG GCGAATTGGT ACTGCCCAAG
    GACCAGAGGC CTAGGAGCCT TTGCCTCTCC TTGGAACACC AAGGGCACCA CCCACCTCAC
    CCAGATGCTC AAGGTCACCC AGCTGTGCCA ATGCTCAAAG ATCCAGGAAG TACGCAGTTC
    TCTCACCACT GGTATCTTAC TGACTGGAAG TCTGGTGACA CCTACCAGTC CTTGTCCAGC
    TCCAGCACTG CCACCGGCAC CACGGTCATT GAGTGCACTC AAGTTCAGGG AAGCTCAGAG
    TCTCTGGCCT CCCCTTCCAC CTCCAGAGCG ACAACACCCT CCCAGCTCTC CATTGAGGTG
    GAAGCCAGGG AAGTAGCCTC TCCTGGGAGG CCCACTGGAC TGATGTCCCC TTCCAGTGGT
    TACTCCAGCC AGTCAGAGAC ACCCACACCC ACTGTCTCCA TGTCCTTGAC CCTGGGCCAC
    TTACCTCCTC CAAGTGCTAG TGTCAGAGTA CGTCCAGTGG TACCAGAAAG GAAGTCATCA
    CTGCCCCCAA CATCACCAAT GGAGAAAATC TGCAAGTCAC GGTTATCATT TGACCTACCA
    CTGACCTCTT CAACCACCCT GGATCTGTCG GGGATGAGTA TCTCCATCCG CAGCAAAACC
    AAGGTGAGCC GCCATCACTC TGATACCAAT TTTGGGGTCA AACTGGCACA GAAAACTAGT
    CCAAACCAAC CGATCATGCC CATGGTTACT CAGTCTGACC TCCGTTCTGT TCGCCTGAGG
    TCAGTCAGCA AGTCTGAGCC AGAAGACGAC ATTGAGAGTC CAGATTACAT AGAGGAACCT
    GGAGCAGAGG AAGTCTTCAC CATGCCAGAG AGAAAAGTGA AACCTCCCAT AGCCGAGAAA
    CCTCCATTGG CCCGAAGGCC TCCAAGTTTG GTCCACAGGC CGCCCTCTCT CCCTGGGGAG
    TATCCACTAA CTTCTCCTAC TATGGCTATG GCATCCAGGA GCTCCATTCC ACACATGAAG
    CAGCTTCCCC AAGACAGCTA CACAGTGTTG CGGAAACCAA AGTCACCCAG CTTCCCCGGG
    GAGTCAACAG CATCCTCCAG CCTTGTCCTC TCACCTCTTG CCAGTTCCTC TGGTGCTTTC
    TTCTCAGGAA CACAGCAACC TCCCCAGGCC AGTGTAGAAG ACGGGGGCCC CAAGGTGAGA
    GCCCTGCCTG AAAGAATCGG CCTCCAGAGC CAGGAAGAAG CTGAGAAAAA GATGACCAAG
    ATTCCACCTC CGGTACCAAA AAAGCCCAGT GTGCTCTACC TGCCTCTCAC CTCCCCTGTA
    GCTCAAATGG ATGCCTGCAT GGCAGAACCA AGGCTACCTT TCAGCCCCAT CATCACCCTG
    GAGGAAGATG GCAAGTGTCC CTCCACTGGT GATGACCAGA AGTCACCTGG TAAAGGGGTG
    ACTTCGCCTC TTCACACTGA CACAGAAAAA GAGGCCATCT CTCCAGGGAG GTCAGTGGAA
    CCGAGTGCTG AAGAGAAAAG TTTAATCAGT GATAAAACAG CCGAATGGAT TGCAGAGGAG
    GAGGATGACG TGTTTGTGGC TTCCCGCACA ACTGAAGATT TATTTACTGT GATACACAGG
    TCCAAAAGAA AGCTACTGGG CTGGAAAGAG ACTGGGGAGG GCTTCACGGG AAGCAAACCC
    AGCTCCCATT CACCAGTGAA GAATACAGCT GATTCTCCCA CCGGTGAGGC TGCTGCTGCC
    CCAGGGCCAA GTAGCAGTGC CTGCCTAGAT GCTGGTAGAA ATGATGATTT CAAGGCCTTG
    CTCCAGAAGA AGGGAAGTAA GGCAACTCCC AGGACCCGCC CCTCAGCGGC CGAGCTGCTG
    AAGACCACTA ACCCTCTGGC TCGAAGGATT ATTGCACAGT TCTCAAAAGA CTACGAACCC
    ACTGACAACC CCAGTACCTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006527790.1
    CDS275..3115
    Translation

    Target ORF information:

    RefSeq Version XM_006527727.3
    Organism Mus musculus(house mouse)
    Definition Mus musculus NHS-like 2 (Nhsl2), transcript variant X13, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006527727.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    ATGATGGGCA ACTCTCACCA CAAGCAACCG AGGAGTAAGA GCCAAAGCAG CAGGATGCAT 
    TCAGCAACAG GTCACAGCAG CAGCCCCACA GGCAGCCTGG CCCGCTCTGC CACCTCAGAC
    ATCAGGCCTG GCCACTCAGC TCCACAAGTT GTTCAGGGAA GAGTTGCTGT TGGGCAGGAA
    GCTCGGTTCC CAAGTCTCAC CTCACCAGGA CTGAGACACT CTTCTAGTGA GCCCGGAGAC
    GCTCACCAGG CACGCAGTGC CTCAGACCAT CCCGGCATGG AGAGCATGGC AGTGGTGTAC
    AGTGTCCCCA GTTCTTGCAA TGGACCAACA GAGTCAACAT TCTCCACTTC CTGGAAGGGA
    GATGCTTTCA CATACATGAC CCCAAGTGCC AGCAGCCAGG GCAATCAAGT CAGTGAAAAT
    GGAAAAAACC CTTCCTCGGG CAATTCTTGG GTCCCTCTGA ACACACTCCC ACCTCTGGTT
    CCTAAGGAGG CGGCCACGCT CTTTGTCACC CGTGATAACC CAGCAGGATG CACTGGGCTA
    CCCAGCTACT CTGAGCACCC CACTCTACGA AGACAGATAC CAGAAAGACC TCCCAAGATT
    GGCCTTCTTG CCCGTGGTAC CTCAAGGCTG GAAACAGGCC CAGGTGGGAC CAACAGGTTC
    CGGGAGCGGT CACTGTCTGT ACCCACAGAC TCGGGTGTTA CCTCAGTGGA CTATGACGAA
    GAACAGAAGA CCAGTGAGAC CCGCATCCTG CCTTATGCCA GTACAAGCTC CGAGGGCAGT
    AACAGTACTG ACAACATTGC AGCCCTCAGC ACTGAGCAGG AGGCACGGCA CAGAAGGCAA
    AGATCCAAAA GTATTTCACT CAAGAAGGCC AAAAAGAAGC CCTCTCCACC CATGCGAAGT
    GTCTCACTCG TCAAAGATGA GCCAGCCCTC CCACCAGAAG GCGAATTGGT ACTGCCCAAG
    GACCAGAGGC CTAGGAGCCT TTGCCTCTCC TTGGAACACC AAGGGCACCA CCCACCTCAC
    CCAGATGCTC AAGGTCACCC AGCTGTGCCA ATGCTCAAAG ATCCAGGAAG TACGCAGTTC
    TCTCACCACT GGTATCTTAC TGACTGGAAG TCTGGTGACA CCTACCAGTC CTTGTCCAGC
    TCCAGCACTG CCACCGGCAC CACGGTCATT GAGTGCACTC AAGTTCAGGG AAGCTCAGAG
    TCTCTGGCCT CCCCTTCCAC CTCCAGAGCG ACAACACCCT CCCAGCTCTC CATTGAGGTG
    GAAGCCAGGG AAGTAGCCTC TCCTGGGAGG CCCACTGGAC TGATGTCCCC TTCCAGTGGT
    TACTCCAGCC AGTCAGAGAC ACCCACACCC ACTGTCTCCA TGTCCTTGAC CCTGGGCCAC
    TTACCTCCTC CAAGTGCTAG TGTCAGAGTA CGTCCAGTGG TACCAGAAAG GAAGTCATCA
    CTGCCCCCAA CATCACCAAT GGAGAAAATC TGCAAGTCAC GGTTATCATT TGACCTACCA
    CTGACCTCTT CAACCACCCT GGATCTGTCG GGGATGAGTA TCTCCATCCG CAGCAAAACC
    AAGGTGAGCC GCCATCACTC TGATACCAAT TTTGGGGTCA AACTGGCACA GAAAACTAGT
    CCAAACCAAC CGATCATGCC CATGGTTACT CAGTCTGACC TCCGTTCTGT TCGCCTGAGG
    TCAGTCAGCA AGTCTGAGCC AGAAGACGAC ATTGAGAGTC CAGATTACAT AGAGGAACCT
    GGAGCAGAGG AAGTCTTCAC CATGCCAGAG AGAAAAGTGA AACCTCCCAT AGCCGAGAAA
    CCTCCATTGG CCCGAAGGCC TCCAAGTTTG GTCCACAGGC CGCCCTCTCT CCCTGGGGAG
    TATCCACTAA CTTCTCCTAC TATGGCTATG GCATCCAGGA GCTCCATTCC ACACATGAAG
    CAGCTTCCCC AAGACAGCTA CACAGTGTTG CGGAAACCAA AGTCACCCAG CTTCCCCGGG
    GAGTCAACAG CATCCTCCAG CCTTGTCCTC TCACCTCTTG CCAGTTCCTC TGGTGCTTTC
    TTCTCAGGAA CACAGCAACC TCCCCAGGCC AGTGTAGAAG ACGGGGGCCC CAAGGTGAGA
    GCCCTGCCTG AAAGAATCGG CCTCCAGAGC CAGGAAGAAG CTGAGAAAAA GATGACCAAG
    ATTCCACCTC CGGTACCAAA AAAGCCCAGT GTGCTCTACC TGCCTCTCAC CTCCCCTGTA
    GCTCAAATGG ATGCCTGCAT GGCAGAACCA AGGCTACCTT TCAGCCCCAT CATCACCCTG
    GAGGAAGATG GCAAGTGTCC CTCCACTGGT GATGACCAGA AGTCACCTGG TAAAGGGGTG
    ACTTCGCCTC TTCACACTGA CACAGAAAAA GAGGCCATCT CTCCAGGGAG GTCAGTGGAA
    CCGAGTGCTG AAGAGAAAAG TTTAATCAGT GATAAAACAG CCGAATGGAT TGCAGAGGAG
    GAGGATGACG TGTTTGTGGC TTCCCGCACA ACTGAAGATT TATTTACTGT GATACACAGG
    TCCAAAAGAA AGCTACTGGG CTGGAAAGAG ACTGGGGAGG GCTTCACGGG AAGCAAACCC
    AGCTCCCATT CACCAGTGAA GAATACAGCT GATTCTCCCA CCGGTGAGGC TGCTGCTGCC
    CCAGGGCCAA GTAGCAGTGC CTGCCTAGAT GCTGGTAGAA ATGATGATTT CAAGGCCTTG
    CTCCAGAAGA AGGGAAGTAA GGCAACTCCC AGGACCCGCC CCTCAGCGGC CGAGCTGCTG
    AAGACCACTA ACCCTCTGGC TCGAAGGATT ATTGCACAGT TCTCAAAAGA CTACGAACCC
    ACTGACAACC CCAGTACCTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu56087
    Clone ID Related Accession (Same CDS sequence) XM_006527728.3 , XM_006527728.4
    Accession Version XM_006527728.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2799bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-21
    Organism Mus musculus(house mouse)
    Product NHS-like protein 2 isoform X14
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000086.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006527728.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    ATGGCAGCAC AGTCTGGAGC TTGGAATGGT CACAGCAGCA GCCCCACAGG CAGCCTGGCC 
    CGCTCTGCCA CCTCAGACAT CAGGCCTGGC CACTCAGCTC CACAAGTTGT TCAGGGAAGA
    GTTGCTGTTG GGCAGGAAGC TCGGTTCCCA AGTCTCACCT CACCAGGACT GAGACACTCT
    TCTAGTGAGC CCGGAGACGC TCACCAGGCA CGCAGTGCCT CAGACCATCC CGGCATGGAG
    AGCATGGCAG TGGTGTACAG TGTCCCCAGT TCTTGCAATG GACCAACAGA GTCAACATTC
    TCCACTTCCT GGAAGGGAGA TGCTTTCACA TACATGACCC CAAGTGCCAG CAGCCAGGGC
    AATCAAGTCA GTGAAAATGG AAAAAACCCT TCCTCGGGCA ATTCTTGGGT CCCTCTGAAC
    ACACTCCCAC CTCTGGTTCC TAAGGAGGCG GCCACGCTCT TTGTCACCCG TGATAACCCA
    GCAGGATGCA CTGGGCTACC CAGCTACTCT GAGCACCCCA CTCTACGAAG ACAGATACCA
    GAAAGACCTC CCAAGATTGG CCTTCTTGCC CGTGGTACCT CAAGGCTGGA AACAGGCCCA
    GGTGGGACCA ACAGGTTCCG GGAGCGGTCA CTGTCTGTAC CCACAGACTC GGGTGTTACC
    TCAGTGGACT ATGACGAAGA ACAGAAGACC AGTGAGACCC GCATCCTGCC TTATGCCAGT
    ACAAGCTCCG AGGGCAGTAA CAGTACTGAC AACATTGCAG CCCTCAGCAC TGAGCAGGAG
    GCACGGCACA GAAGGCAAAG ATCCAAAAGT ATTTCACTCA AGAAGGCCAA AAAGAAGCCC
    TCTCCACCCA TGCGAAGTGT CTCACTCGTC AAAGATGAGC CAGCCCTCCC ACCAGAAGGC
    GAATTGGTAC TGCCCAAGGA CCAGAGGCCT AGGAGCCTTT GCCTCTCCTT GGAACACCAA
    GGGCACCACC CACCTCACCC AGATGCTCAA GGTCACCCAG CTGTGCCAAT GCTCAAAGAT
    CCAGGAAGTA CGCAGTTCTC TCACCACTGG TATCTTACTG ACTGGAAGTC TGGTGACACC
    TACCAGTCCT TGTCCAGCTC CAGCACTGCC ACCGGCACCA CGGTCATTGA GTGCACTCAA
    GTTCAGGGAA GCTCAGAGTC TCTGGCCTCC CCTTCCACCT CCAGAGCGAC AACACCCTCC
    CAGCTCTCCA TTGAGGTGGA AGCCAGGGAA GTAGCCTCTC CTGGGAGGCC CACTGGACTG
    ATGTCCCCTT CCAGTGGTTA CTCCAGCCAG TCAGAGACAC CCACACCCAC TGTCTCCATG
    TCCTTGACCC TGGGCCACTT ACCTCCTCCA AGTGCTAGTG TCAGAGTACG TCCAGTGGTA
    CCAGAAAGGA AGTCATCACT GCCCCCAACA TCACCAATGG AGAAAATCTG CAAGTCACGG
    TTATCATTTG ACCTACCACT GACCTCTTCA ACCACCCTGG ATCTGTCGGG GATGAGTATC
    TCCATCCGCA GCAAAACCAA GGTGAGCCGC CATCACTCTG ATACCAATTT TGGGGTCAAA
    CTGGCACAGA AAACTAGTCC AAACCAACCG ATCATGCCCA TGGTTACTCA GTCTGACCTC
    CGTTCTGTTC GCCTGAGGTC AGTCAGCAAG TCTGAGCCAG AAGACGACAT TGAGAGTCCA
    GATTACATAG AGGAACCTGG AGCAGAGGAA GTCTTCACCA TGCCAGAGAG AAAAGTGAAA
    CCTCCCATAG CCGAGAAACC TCCATTGGCC CGAAGGCCTC CAAGTTTGGT CCACAGGCCG
    CCCTCTCTCC CTGGGGAGTA TCCACTAACT TCTCCTACTA TGGCTATGGC ATCCAGGAGC
    TCCATTCCAC ACATGAAGCA GCTTCCCCAA GACAGCTACA CAGTGTTGCG GAAACCAAAG
    TCACCCAGCT TCCCCGGGGA GTCAACAGCA TCCTCCAGCC TTGTCCTCTC ACCTCTTGCC
    AGTTCCTCTG GTGCTTTCTT CTCAGGAACA CAGCAACCTC CCCAGGCCAG TGTAGAAGAC
    GGGGGCCCCA AGGTGAGAGC CCTGCCTGAA AGAATCGGCC TCCAGAGCCA GGAAGAAGCT
    GAGAAAAAGA TGACCAAGAT TCCACCTCCG GTACCAAAAA AGCCCAGTGT GCTCTACCTG
    CCTCTCACCT CCCCTGTAGC TCAAATGGAT GCCTGCATGG CAGAACCAAG GCTACCTTTC
    AGCCCCATCA TCACCCTGGA GGAAGATGGC AAGTGTCCCT CCACTGGTGA TGACCAGAAG
    TCACCTGGTA AAGGGGTGAC TTCGCCTCTT CACACTGACA CAGAAAAAGA GGCCATCTCT
    CCAGGGAGGT CAGTGGAACC GAGTGCTGAA GAGAAAAGTT TAATCAGTGA TAAAACAGCC
    GAATGGATTG CAGAGGAGGA GGATGACGTG TTTGTGGCTT CCCGCACAAC TGAAGATTTA
    TTTACTGTGA TACACAGGTC CAAAAGAAAG CTACTGGGCT GGAAAGAGAC TGGGGAGGGC
    TTCACGGGAA GCAAACCCAG CTCCCATTCA CCAGTGAAGA ATACAGCTGA TTCTCCCACC
    GGTGAGGCTG CTGCTGCCCC AGGGCCAAGT AGCAGTGCCT GCCTAGATGC TGGTAGAAAT
    GATGATTTCA AGGCCTTGCT CCAGAAGAAG GGAAGTAAGG CAACTCCCAG GACCCGCCCC
    TCAGCGGCCG AGCTGCTGAA GACCACTAAC CCTCTGGCTC GAAGGATTAT TGCACAGTTC
    TCAAAAGACT ACGAACCCAC TGACAACCCC AGTACCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006527791.1
    CDS153..2951
    Translation

    Target ORF information:

    RefSeq Version XM_006527728.3
    Organism Mus musculus(house mouse)
    Definition Mus musculus NHS-like 2 (Nhsl2), transcript variant X14, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006527728.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    ATGGCAGCAC AGTCTGGAGC TTGGAATGGT CACAGCAGCA GCCCCACAGG CAGCCTGGCC 
    CGCTCTGCCA CCTCAGACAT CAGGCCTGGC CACTCAGCTC CACAAGTTGT TCAGGGAAGA
    GTTGCTGTTG GGCAGGAAGC TCGGTTCCCA AGTCTCACCT CACCAGGACT GAGACACTCT
    TCTAGTGAGC CCGGAGACGC TCACCAGGCA CGCAGTGCCT CAGACCATCC CGGCATGGAG
    AGCATGGCAG TGGTGTACAG TGTCCCCAGT TCTTGCAATG GACCAACAGA GTCAACATTC
    TCCACTTCCT GGAAGGGAGA TGCTTTCACA TACATGACCC CAAGTGCCAG CAGCCAGGGC
    AATCAAGTCA GTGAAAATGG AAAAAACCCT TCCTCGGGCA ATTCTTGGGT CCCTCTGAAC
    ACACTCCCAC CTCTGGTTCC TAAGGAGGCG GCCACGCTCT TTGTCACCCG TGATAACCCA
    GCAGGATGCA CTGGGCTACC CAGCTACTCT GAGCACCCCA CTCTACGAAG ACAGATACCA
    GAAAGACCTC CCAAGATTGG CCTTCTTGCC CGTGGTACCT CAAGGCTGGA AACAGGCCCA
    GGTGGGACCA ACAGGTTCCG GGAGCGGTCA CTGTCTGTAC CCACAGACTC GGGTGTTACC
    TCAGTGGACT ATGACGAAGA ACAGAAGACC AGTGAGACCC GCATCCTGCC TTATGCCAGT
    ACAAGCTCCG AGGGCAGTAA CAGTACTGAC AACATTGCAG CCCTCAGCAC TGAGCAGGAG
    GCACGGCACA GAAGGCAAAG ATCCAAAAGT ATTTCACTCA AGAAGGCCAA AAAGAAGCCC
    TCTCCACCCA TGCGAAGTGT CTCACTCGTC AAAGATGAGC CAGCCCTCCC ACCAGAAGGC
    GAATTGGTAC TGCCCAAGGA CCAGAGGCCT AGGAGCCTTT GCCTCTCCTT GGAACACCAA
    GGGCACCACC CACCTCACCC AGATGCTCAA GGTCACCCAG CTGTGCCAAT GCTCAAAGAT
    CCAGGAAGTA CGCAGTTCTC TCACCACTGG TATCTTACTG ACTGGAAGTC TGGTGACACC
    TACCAGTCCT TGTCCAGCTC CAGCACTGCC ACCGGCACCA CGGTCATTGA GTGCACTCAA
    GTTCAGGGAA GCTCAGAGTC TCTGGCCTCC CCTTCCACCT CCAGAGCGAC AACACCCTCC
    CAGCTCTCCA TTGAGGTGGA AGCCAGGGAA GTAGCCTCTC CTGGGAGGCC CACTGGACTG
    ATGTCCCCTT CCAGTGGTTA CTCCAGCCAG TCAGAGACAC CCACACCCAC TGTCTCCATG
    TCCTTGACCC TGGGCCACTT ACCTCCTCCA AGTGCTAGTG TCAGAGTACG TCCAGTGGTA
    CCAGAAAGGA AGTCATCACT GCCCCCAACA TCACCAATGG AGAAAATCTG CAAGTCACGG
    TTATCATTTG ACCTACCACT GACCTCTTCA ACCACCCTGG ATCTGTCGGG GATGAGTATC
    TCCATCCGCA GCAAAACCAA GGTGAGCCGC CATCACTCTG ATACCAATTT TGGGGTCAAA
    CTGGCACAGA AAACTAGTCC AAACCAACCG ATCATGCCCA TGGTTACTCA GTCTGACCTC
    CGTTCTGTTC GCCTGAGGTC AGTCAGCAAG TCTGAGCCAG AAGACGACAT TGAGAGTCCA
    GATTACATAG AGGAACCTGG AGCAGAGGAA GTCTTCACCA TGCCAGAGAG AAAAGTGAAA
    CCTCCCATAG CCGAGAAACC TCCATTGGCC CGAAGGCCTC CAAGTTTGGT CCACAGGCCG
    CCCTCTCTCC CTGGGGAGTA TCCACTAACT TCTCCTACTA TGGCTATGGC ATCCAGGAGC
    TCCATTCCAC ACATGAAGCA GCTTCCCCAA GACAGCTACA CAGTGTTGCG GAAACCAAAG
    TCACCCAGCT TCCCCGGGGA GTCAACAGCA TCCTCCAGCC TTGTCCTCTC ACCTCTTGCC
    AGTTCCTCTG GTGCTTTCTT CTCAGGAACA CAGCAACCTC CCCAGGCCAG TGTAGAAGAC
    GGGGGCCCCA AGGTGAGAGC CCTGCCTGAA AGAATCGGCC TCCAGAGCCA GGAAGAAGCT
    GAGAAAAAGA TGACCAAGAT TCCACCTCCG GTACCAAAAA AGCCCAGTGT GCTCTACCTG
    CCTCTCACCT CCCCTGTAGC TCAAATGGAT GCCTGCATGG CAGAACCAAG GCTACCTTTC
    AGCCCCATCA TCACCCTGGA GGAAGATGGC AAGTGTCCCT CCACTGGTGA TGACCAGAAG
    TCACCTGGTA AAGGGGTGAC TTCGCCTCTT CACACTGACA CAGAAAAAGA GGCCATCTCT
    CCAGGGAGGT CAGTGGAACC GAGTGCTGAA GAGAAAAGTT TAATCAGTGA TAAAACAGCC
    GAATGGATTG CAGAGGAGGA GGATGACGTG TTTGTGGCTT CCCGCACAAC TGAAGATTTA
    TTTACTGTGA TACACAGGTC CAAAAGAAAG CTACTGGGCT GGAAAGAGAC TGGGGAGGGC
    TTCACGGGAA GCAAACCCAG CTCCCATTCA CCAGTGAAGA ATACAGCTGA TTCTCCCACC
    GGTGAGGCTG CTGCTGCCCC AGGGCCAAGT AGCAGTGCCT GCCTAGATGC TGGTAGAAAT
    GATGATTTCA AGGCCTTGCT CCAGAAGAAG GGAAGTAAGG CAACTCCCAG GACCCGCCCC
    TCAGCGGCCG AGCTGCTGAA GACCACTAAC CCTCTGGCTC GAAGGATTAT TGCACAGTTC
    TCAAAAGACT ACGAACCCAC TGACAACCCC AGTACCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu56088
    Clone ID Related Accession (Same CDS sequence) XM_006527729.3 , XM_030251165.1 , XM_006527729.4
    Accession Version XM_006527729.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2565bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-21
    Organism Mus musculus(house mouse)
    Product NHS-like protein 2 isoform X15
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000086.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006527729.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    ATGGAGAGCA TGGCAGTGGT GTACAGTGTC CCCAGTTCTT GCAATGGACC AACAGAGTCA 
    ACATTCTCCA CTTCCTGGAA GGGAGATGCT TTCACATACA TGACCCCAAG TGCCAGCAGC
    CAGGGCAATC AAGTCAGTGA AAATGGAAAA AACCCTTCCT CGGGCAATTC TTGGGTCCCT
    CTGAACACAC TCCCACCTCT GGTTCCTAAG GAGGCGGCCA CGCTCTTTGT CACCCGTGAT
    AACCCAGCAG GATGCACTGG GCTACCCAGC TACTCTGAGC ACCCCACTCT ACGAAGACAG
    ATACCAGAAA GACCTCCCAA GATTGGCCTT CTTGCCCGTG GTACCTCAAG GCTGGAAACA
    GGCCCAGGTG GGACCAACAG GTTCCGGGAG CGGTCACTGT CTGTACCCAC AGACTCGGGT
    GTTACCTCAG TGGACTATGA CGAAGAACAG AAGACCAGTG AGACCCGCAT CCTGCCTTAT
    GCCAGTACAA GCTCCGAGGG CAGTAACAGT ACTGACAACA TTGCAGCCCT CAGCACTGAG
    CAGGAGGCAC GGCACAGAAG GCAAAGATCC AAAAGTATTT CACTCAAGAA GGCCAAAAAG
    AAGCCCTCTC CACCCATGCG AAGTGTCTCA CTCGTCAAAG ATGAGCCAGC CCTCCCACCA
    GAAGGCGAAT TGGTACTGCC CAAGGACCAG AGGCCTAGGA GCCTTTGCCT CTCCTTGGAA
    CACCAAGGGC ACCACCCACC TCACCCAGAT GCTCAAGGTC ACCCAGCTGT GCCAATGCTC
    AAAGATCCAG GAAGTACGCA GTTCTCTCAC CACTGGTATC TTACTGACTG GAAGTCTGGT
    GACACCTACC AGTCCTTGTC CAGCTCCAGC ACTGCCACCG GCACCACGGT CATTGAGTGC
    ACTCAAGTTC AGGGAAGCTC AGAGTCTCTG GCCTCCCCTT CCACCTCCAG AGCGACAACA
    CCCTCCCAGC TCTCCATTGA GGTGGAAGCC AGGGAAGTAG CCTCTCCTGG GAGGCCCACT
    GGACTGATGT CCCCTTCCAG TGGTTACTCC AGCCAGTCAG AGACACCCAC ACCCACTGTC
    TCCATGTCCT TGACCCTGGG CCACTTACCT CCTCCAAGTG CTAGTGTCAG AGTACGTCCA
    GTGGTACCAG AAAGGAAGTC ATCACTGCCC CCAACATCAC CAATGGAGAA AATCTGCAAG
    TCACGGTTAT CATTTGACCT ACCACTGACC TCTTCAACCA CCCTGGATCT GTCGGGGATG
    AGTATCTCCA TCCGCAGCAA AACCAAGGTG AGCCGCCATC ACTCTGATAC CAATTTTGGG
    GTCAAACTGG CACAGAAAAC TAGTCCAAAC CAACCGATCA TGCCCATGGT TACTCAGTCT
    GACCTCCGTT CTGTTCGCCT GAGGTCAGTC AGCAAGTCTG AGCCAGAAGA CGACATTGAG
    AGTCCAGATT ACATAGAGGA ACCTGGAGCA GAGGAAGTCT TCACCATGCC AGAGAGAAAA
    GTGAAACCTC CCATAGCCGA GAAACCTCCA TTGGCCCGAA GGCCTCCAAG TTTGGTCCAC
    AGGCCGCCCT CTCTCCCTGG GGAGTATCCA CTAACTTCTC CTACTATGGC TATGGCATCC
    AGGAGCTCCA TTCCACACAT GAAGCAGCTT CCCCAAGACA GCTACACAGT GTTGCGGAAA
    CCAAAGTCAC CCAGCTTCCC CGGGGAGTCA ACAGCATCCT CCAGCCTTGT CCTCTCACCT
    CTTGCCAGTT CCTCTGGTGC TTTCTTCTCA GGAACACAGC AACCTCCCCA GGCCAGTGTA
    GAAGACGGGG GCCCCAAGGT GAGAGCCCTG CCTGAAAGAA TCGGCCTCCA GAGCCAGGAA
    GAAGCTGAGA AAAAGATGAC CAAGATTCCA CCTCCGGTAC CAAAAAAGCC CAGTGTGCTC
    TACCTGCCTC TCACCTCCCC TGTAGCTCAA ATGGATGCCT GCATGGCAGA ACCAAGGCTA
    CCTTTCAGCC CCATCATCAC CCTGGAGGAA GATGGCAAGT GTCCCTCCAC TGGTGATGAC
    CAGAAGTCAC CTGGTAAAGG GGTGACTTCG CCTCTTCACA CTGACACAGA AAAAGAGGCC
    ATCTCTCCAG GGAGGTCAGT GGAACCGAGT GCTGAAGAGA AAAGTTTAAT CAGTGATAAA
    ACAGCCGAAT GGATTGCAGA GGAGGAGGAT GACGTGTTTG TGGCTTCCCG CACAACTGAA
    GATTTATTTA CTGTGATACA CAGGTCCAAA AGAAAGCTAC TGGGCTGGAA AGAGACTGGG
    GAGGGCTTCA CGGGAAGCAA ACCCAGCTCC CATTCACCAG TGAAGAATAC AGCTGATTCT
    CCCACCGGTG AGGCTGCTGC TGCCCCAGGG CCAAGTAGCA GTGCCTGCCT AGATGCTGGT
    AGAAATGATG ATTTCAAGGC CTTGCTCCAG AAGAAGGGAA GTAAGGCAAC TCCCAGGACC
    CGCCCCTCAG CGGCCGAGCT GCTGAAGACC ACTAACCCTC TGGCTCGAAG GATTATTGCA
    CAGTTCTCAA AAGACTACGA ACCCACTGAC AACCCCAGTA CCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006527792.1
    CDS2006..4570
    Translation

    Target ORF information:

    RefSeq Version XM_006527729.3
    Organism Mus musculus(house mouse)
    Definition Mus musculus NHS-like 2 (Nhsl2), transcript variant X15, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006527729.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    ATGGAGAGCA TGGCAGTGGT GTACAGTGTC CCCAGTTCTT GCAATGGACC AACAGAGTCA 
    ACATTCTCCA CTTCCTGGAA GGGAGATGCT TTCACATACA TGACCCCAAG TGCCAGCAGC
    CAGGGCAATC AAGTCAGTGA AAATGGAAAA AACCCTTCCT CGGGCAATTC TTGGGTCCCT
    CTGAACACAC TCCCACCTCT GGTTCCTAAG GAGGCGGCCA CGCTCTTTGT CACCCGTGAT
    AACCCAGCAG GATGCACTGG GCTACCCAGC TACTCTGAGC ACCCCACTCT ACGAAGACAG
    ATACCAGAAA GACCTCCCAA GATTGGCCTT CTTGCCCGTG GTACCTCAAG GCTGGAAACA
    GGCCCAGGTG GGACCAACAG GTTCCGGGAG CGGTCACTGT CTGTACCCAC AGACTCGGGT
    GTTACCTCAG TGGACTATGA CGAAGAACAG AAGACCAGTG AGACCCGCAT CCTGCCTTAT
    GCCAGTACAA GCTCCGAGGG CAGTAACAGT ACTGACAACA TTGCAGCCCT CAGCACTGAG
    CAGGAGGCAC GGCACAGAAG GCAAAGATCC AAAAGTATTT CACTCAAGAA GGCCAAAAAG
    AAGCCCTCTC CACCCATGCG AAGTGTCTCA CTCGTCAAAG ATGAGCCAGC CCTCCCACCA
    GAAGGCGAAT TGGTACTGCC CAAGGACCAG AGGCCTAGGA GCCTTTGCCT CTCCTTGGAA
    CACCAAGGGC ACCACCCACC TCACCCAGAT GCTCAAGGTC ACCCAGCTGT GCCAATGCTC
    AAAGATCCAG GAAGTACGCA GTTCTCTCAC CACTGGTATC TTACTGACTG GAAGTCTGGT
    GACACCTACC AGTCCTTGTC CAGCTCCAGC ACTGCCACCG GCACCACGGT CATTGAGTGC
    ACTCAAGTTC AGGGAAGCTC AGAGTCTCTG GCCTCCCCTT CCACCTCCAG AGCGACAACA
    CCCTCCCAGC TCTCCATTGA GGTGGAAGCC AGGGAAGTAG CCTCTCCTGG GAGGCCCACT
    GGACTGATGT CCCCTTCCAG TGGTTACTCC AGCCAGTCAG AGACACCCAC ACCCACTGTC
    TCCATGTCCT TGACCCTGGG CCACTTACCT CCTCCAAGTG CTAGTGTCAG AGTACGTCCA
    GTGGTACCAG AAAGGAAGTC ATCACTGCCC CCAACATCAC CAATGGAGAA AATCTGCAAG
    TCACGGTTAT CATTTGACCT ACCACTGACC TCTTCAACCA CCCTGGATCT GTCGGGGATG
    AGTATCTCCA TCCGCAGCAA AACCAAGGTG AGCCGCCATC ACTCTGATAC CAATTTTGGG
    GTCAAACTGG CACAGAAAAC TAGTCCAAAC CAACCGATCA TGCCCATGGT TACTCAGTCT
    GACCTCCGTT CTGTTCGCCT GAGGTCAGTC AGCAAGTCTG AGCCAGAAGA CGACATTGAG
    AGTCCAGATT ACATAGAGGA ACCTGGAGCA GAGGAAGTCT TCACCATGCC AGAGAGAAAA
    GTGAAACCTC CCATAGCCGA GAAACCTCCA TTGGCCCGAA GGCCTCCAAG TTTGGTCCAC
    AGGCCGCCCT CTCTCCCTGG GGAGTATCCA CTAACTTCTC CTACTATGGC TATGGCATCC
    AGGAGCTCCA TTCCACACAT GAAGCAGCTT CCCCAAGACA GCTACACAGT GTTGCGGAAA
    CCAAAGTCAC CCAGCTTCCC CGGGGAGTCA ACAGCATCCT CCAGCCTTGT CCTCTCACCT
    CTTGCCAGTT CCTCTGGTGC TTTCTTCTCA GGAACACAGC AACCTCCCCA GGCCAGTGTA
    GAAGACGGGG GCCCCAAGGT GAGAGCCCTG CCTGAAAGAA TCGGCCTCCA GAGCCAGGAA
    GAAGCTGAGA AAAAGATGAC CAAGATTCCA CCTCCGGTAC CAAAAAAGCC CAGTGTGCTC
    TACCTGCCTC TCACCTCCCC TGTAGCTCAA ATGGATGCCT GCATGGCAGA ACCAAGGCTA
    CCTTTCAGCC CCATCATCAC CCTGGAGGAA GATGGCAAGT GTCCCTCCAC TGGTGATGAC
    CAGAAGTCAC CTGGTAAAGG GGTGACTTCG CCTCTTCACA CTGACACAGA AAAAGAGGCC
    ATCTCTCCAG GGAGGTCAGT GGAACCGAGT GCTGAAGAGA AAAGTTTAAT CAGTGATAAA
    ACAGCCGAAT GGATTGCAGA GGAGGAGGAT GACGTGTTTG TGGCTTCCCG CACAACTGAA
    GATTTATTTA CTGTGATACA CAGGTCCAAA AGAAAGCTAC TGGGCTGGAA AGAGACTGGG
    GAGGGCTTCA CGGGAAGCAA ACCCAGCTCC CATTCACCAG TGAAGAATAC AGCTGATTCT
    CCCACCGGTG AGGCTGCTGC TGCCCCAGGG CCAAGTAGCA GTGCCTGCCT AGATGCTGGT
    AGAAATGATG ATTTCAAGGC CTTGCTCCAG AAGAAGGGAA GTAAGGCAAC TCCCAGGACC
    CGCCCCTCAG CGGCCGAGCT GCTGAAGACC ACTAACCCTC TGGCTCGAAG GATTATTGCA
    CAGTTCTCAA AAGACTACGA ACCCACTGAC AACCCCAGTA CCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu56083
    Clone ID Related Accession (Same CDS sequence) XM_006527724.4 , XM_006527724.3
    Accession Version XM_006527724.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2973bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product NHS-like protein 2 isoform X12
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000086.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006527724.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGATGGGCA ACTCTCACCA CAAGCAACCG AGGAGTAAGA GCCAAAGCAG CAGGATGCAT 
    TCAGCAACAG GGCAGCAGTT TGATAGACAT GCAAGTTTTC GACACTCGTT GTTTAACACA
    GAGACAGCCG TGAACCCCAA GTCCACCCTG AGGCGGAGGC GGACCATTAT TGGATTCTCT
    AACTTTTCTC AGCGAGACCA AGGTCACAGC AGCAGCCCCA CAGGCAGCCT GGCCCGCTCT
    GCCACCTCAG ACATCAGGCC TGGCCACTCA GCTCCACAAG TTGTTCAGGG AAGAGTTGCT
    GTTGGGCAGG AAGCTCGGTT CCCAAGTCTC ACCTCACCAG GACTGAGACA CTCTTCTAGT
    GAGCCCGGAG ACGCTCACCA GGCACGCAGT GCCTCAGACC ATCCCGGCAT GGAGAGCATG
    GCAGTGGTGT ACAGTGTCCC CAGTTCTTGC AATGGACCAA CAGAGTCAAC ATTCTCCACT
    TCCTGGAAGG GAGATGCTTT CACATACATG ACCCCAAGTG CCAGCAGCCA GGGCAATCAA
    GTCAGTGAAA ATGGAAAAAA CCCTTCCTCG GGCAATTCTT GGGTCCCTCT GAACACACTC
    CCACCTCTGG TTCCTAAGGA GGCGGCCACG CTCTTTGTCA CCCGTGATAA CCCAGCAGGA
    TGCACTGGGC TACCCAGCTA CTCTGAGCAC CCCACTCTAC GAAGACAGAT ACCAGAAAGA
    CCTCCCAAGA TTGGCCTTCT TGCCCGTGGT ACCTCAAGGC TGGAAACAGG CCCAGGTGGG
    ACCAACAGGT TCCGGGAGCG GTCACTGTCT GTACCCACAG ACTCGGGTGT TACCTCAGTG
    GACTATGACG AAGAACAGAA GACCAGTGAG ACCCGCATCC TGCCTTATGC CAGTACAAGC
    TCCGAGGGCA GTAACAGTAC TGACAACATT GCAGCCCTCA GCACTGAGCA GGAGGCACGG
    CACAGAAGGC AAAGATCCAA AAGTATTTCA CTCAAGAAGG CCAAAAAGAA GCCCTCTCCA
    CCCATGCGAA GTGTCTCACT CGTCAAAGAT GAGCCAGCCC TCCCACCAGA AGGCGAATTG
    GTACTGCCCA AGGACCAGAG GCCTAGGAGC CTTTGCCTCT CCTTGGAACA CCAAGGGCAC
    CACCCACCTC ACCCAGATGC TCAAGGTCAC CCAGCTGTGC CAATGCTCAA AGATCCAGGA
    AGTACGCAGT TCTCTCACCA CTGGTATCTT ACTGACTGGA AGTCTGGTGA CACCTACCAG
    TCCTTGTCCA GCTCCAGCAC TGCCACCGGC ACCACGGTCA TTGAGTGCAC TCAAGTTCAG
    GGAAGCTCAG AGTCTCTGGC CTCCCCTTCC ACCTCCAGAG CGACAACACC CTCCCAGCTC
    TCCATTGAGG TGGAAGCCAG GGAAGTAGCC TCTCCTGGGA GGCCCACTGG ACTGATGTCC
    CCTTCCAGTG GTTACTCCAG CCAGTCAGAG ACACCCACAC CCACTGTCTC CATGTCCTTG
    ACCCTGGGCC ACTTACCTCC TCCAAGTGCT AGTGTCAGAG TACGTCCAGT GGTACCAGAA
    AGGAAGTCAT CACTGCCCCC AACATCACCA ATGGAGAAAA TCTGCAAGTC ACGGTTATCA
    TTTGACCTAC CACTGACCTC TTCAACCACC CTGGATCTGT CGGGGATGAG TATCTCCATC
    CGCAGCAAAA CCAAGGTGAG CCGCCATCAC TCTGATACCA ATTTTGGGGT CAAACTGGCA
    CAGAAAACTA GTCCAAACCA ACCGATCATG CCCATGGTTA CTCAGTCTGA CCTCCGTTCT
    GTTCGCCTGA GGTCAGTCAG CAAGTCTGAG CCAGAAGACG ACATTGAGAG TCCAGATTAC
    ATAGAGGAAC CTGGAGCAGA GGAAGTCTTC ACCATGCCAG AGAGAAAAGT GAAACCTCCC
    ATAGCCGAGA AACCTCCATT GGCCCGAAGG CCTCCAAGTT TGGTCCACAG GCCGCCCTCT
    CTCCCTGGGG AGTATCCACT AACTTCTCCT ACTATGGCTA TGGCATCCAG GAGCTCCATT
    CCACACATGA AGCAGCTTCC CCAAGACAGC TACACAGTGT TGCGGAAACC AAAGTCACCC
    AGCTTCCCCG GGGAGTCAAC AGCATCCTCC AGCCTTGTCC TCTCACCTCT TGCCAGTTCC
    TCTGGTGCTT TCTTCTCAGG AACACAGCAA CCTCCCCAGG CCAGTGTAGA AGACGGGGGC
    CCCAAGGTGA GAGCCCTGCC TGAAAGAATC GGCCTCCAGA GCCAGGAAGA AGCTGAGAAA
    AAGATGACCA AGATTCCACC TCCGGTACCA AAAAAGCCCA GTGTGCTCTA CCTGCCTCTC
    ACCTCCCCTG TAGCTCAAAT GGATGCCTGC ATGGCAGAAC CAAGGCTACC TTTCAGCCCC
    ATCATCACCC TGGAGGAAGA TGGCAAGTGT CCCTCCACTG GTGATGACCA GAAGTCACCT
    GGTAAAGGGG TGACTTCGCC TCTTCACACT GACACAGAAA AAGAGGCCAT CTCTCCAGGG
    AGGTCAGTGG AACCGAGTGC TGAAGAGAAA AGTTTAATCA GTGATAAAAC AGCCGAATGG
    ATTGCAGAGG AGGAGGATGA CGTGTTTGTG GCTTCCCGCA CAACTGAAGA TTTATTTACT
    GTGATACACA GGTCCAAAAG AAAGCTACTG GGCTGGAAAG AGACTGGGGA GGGCTTCACG
    GGAAGCAAAC CCAGCTCCCA TTCACCAGTG AAGAATACAG CTGATTCTCC CACCGGTGAG
    GCTGCTGCTG CCCCAGGGCC AAGTAGCAGT GCCTGCCTAG ATGCTGGTAG AAATGATGAT
    TTCAAGGCCT TGCTCCAGAA GAAGGGAAGT AAGGCAACTC CCAGGACCCG CCCCTCAGCG
    GCCGAGCTGC TGAAGACCAC TAACCCTCTG GCTCGAAGGA TTATTGCACA GTTCTCAAAA
    GACTACGAAC CCACTGACAA CCCCAGTACC TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006527787.1
    CDS9856..12828
    Translation

    Target ORF information:

    RefSeq Version XM_006527724.4
    Organism Mus musculus(house mouse)
    Definition Mus musculus NHS-like 2 (Nhsl2), transcript variant X12, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006527724.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGATGGGCA ACTCTCACCA CAAGCAACCG AGGAGTAAGA GCCAAAGCAG CAGGATGCAT 
    TCAGCAACAG GGCAGCAGTT TGATAGACAT GCAAGTTTTC GACACTCGTT GTTTAACACA
    GAGACAGCCG TGAACCCCAA GTCCACCCTG AGGCGGAGGC GGACCATTAT TGGATTCTCT
    AACTTTTCTC AGCGAGACCA AGGTCACAGC AGCAGCCCCA CAGGCAGCCT GGCCCGCTCT
    GCCACCTCAG ACATCAGGCC TGGCCACTCA GCTCCACAAG TTGTTCAGGG AAGAGTTGCT
    GTTGGGCAGG AAGCTCGGTT CCCAAGTCTC ACCTCACCAG GACTGAGACA CTCTTCTAGT
    GAGCCCGGAG ACGCTCACCA GGCACGCAGT GCCTCAGACC ATCCCGGCAT GGAGAGCATG
    GCAGTGGTGT ACAGTGTCCC CAGTTCTTGC AATGGACCAA CAGAGTCAAC ATTCTCCACT
    TCCTGGAAGG GAGATGCTTT CACATACATG ACCCCAAGTG CCAGCAGCCA GGGCAATCAA
    GTCAGTGAAA ATGGAAAAAA CCCTTCCTCG GGCAATTCTT GGGTCCCTCT GAACACACTC
    CCACCTCTGG TTCCTAAGGA GGCGGCCACG CTCTTTGTCA CCCGTGATAA CCCAGCAGGA
    TGCACTGGGC TACCCAGCTA CTCTGAGCAC CCCACTCTAC GAAGACAGAT ACCAGAAAGA
    CCTCCCAAGA TTGGCCTTCT TGCCCGTGGT ACCTCAAGGC TGGAAACAGG CCCAGGTGGG
    ACCAACAGGT TCCGGGAGCG GTCACTGTCT GTACCCACAG ACTCGGGTGT TACCTCAGTG
    GACTATGACG AAGAACAGAA GACCAGTGAG ACCCGCATCC TGCCTTATGC CAGTACAAGC
    TCCGAGGGCA GTAACAGTAC TGACAACATT GCAGCCCTCA GCACTGAGCA GGAGGCACGG
    CACAGAAGGC AAAGATCCAA AAGTATTTCA CTCAAGAAGG CCAAAAAGAA GCCCTCTCCA
    CCCATGCGAA GTGTCTCACT CGTCAAAGAT GAGCCAGCCC TCCCACCAGA AGGCGAATTG
    GTACTGCCCA AGGACCAGAG GCCTAGGAGC CTTTGCCTCT CCTTGGAACA CCAAGGGCAC
    CACCCACCTC ACCCAGATGC TCAAGGTCAC CCAGCTGTGC CAATGCTCAA AGATCCAGGA
    AGTACGCAGT TCTCTCACCA CTGGTATCTT ACTGACTGGA AGTCTGGTGA CACCTACCAG
    TCCTTGTCCA GCTCCAGCAC TGCCACCGGC ACCACGGTCA TTGAGTGCAC TCAAGTTCAG
    GGAAGCTCAG AGTCTCTGGC CTCCCCTTCC ACCTCCAGAG CGACAACACC CTCCCAGCTC
    TCCATTGAGG TGGAAGCCAG GGAAGTAGCC TCTCCTGGGA GGCCCACTGG ACTGATGTCC
    CCTTCCAGTG GTTACTCCAG CCAGTCAGAG ACACCCACAC CCACTGTCTC CATGTCCTTG
    ACCCTGGGCC ACTTACCTCC TCCAAGTGCT AGTGTCAGAG TACGTCCAGT GGTACCAGAA
    AGGAAGTCAT CACTGCCCCC AACATCACCA ATGGAGAAAA TCTGCAAGTC ACGGTTATCA
    TTTGACCTAC CACTGACCTC TTCAACCACC CTGGATCTGT CGGGGATGAG TATCTCCATC
    CGCAGCAAAA CCAAGGTGAG CCGCCATCAC TCTGATACCA ATTTTGGGGT CAAACTGGCA
    CAGAAAACTA GTCCAAACCA ACCGATCATG CCCATGGTTA CTCAGTCTGA CCTCCGTTCT
    GTTCGCCTGA GGTCAGTCAG CAAGTCTGAG CCAGAAGACG ACATTGAGAG TCCAGATTAC
    ATAGAGGAAC CTGGAGCAGA GGAAGTCTTC ACCATGCCAG AGAGAAAAGT GAAACCTCCC
    ATAGCCGAGA AACCTCCATT GGCCCGAAGG CCTCCAAGTT TGGTCCACAG GCCGCCCTCT
    CTCCCTGGGG AGTATCCACT AACTTCTCCT ACTATGGCTA TGGCATCCAG GAGCTCCATT
    CCACACATGA AGCAGCTTCC CCAAGACAGC TACACAGTGT TGCGGAAACC AAAGTCACCC
    AGCTTCCCCG GGGAGTCAAC AGCATCCTCC AGCCTTGTCC TCTCACCTCT TGCCAGTTCC
    TCTGGTGCTT TCTTCTCAGG AACACAGCAA CCTCCCCAGG CCAGTGTAGA AGACGGGGGC
    CCCAAGGTGA GAGCCCTGCC TGAAAGAATC GGCCTCCAGA GCCAGGAAGA AGCTGAGAAA
    AAGATGACCA AGATTCCACC TCCGGTACCA AAAAAGCCCA GTGTGCTCTA CCTGCCTCTC
    ACCTCCCCTG TAGCTCAAAT GGATGCCTGC ATGGCAGAAC CAAGGCTACC TTTCAGCCCC
    ATCATCACCC TGGAGGAAGA TGGCAAGTGT CCCTCCACTG GTGATGACCA GAAGTCACCT
    GGTAAAGGGG TGACTTCGCC TCTTCACACT GACACAGAAA AAGAGGCCAT CTCTCCAGGG
    AGGTCAGTGG AACCGAGTGC TGAAGAGAAA AGTTTAATCA GTGATAAAAC AGCCGAATGG
    ATTGCAGAGG AGGAGGATGA CGTGTTTGTG GCTTCCCGCA CAACTGAAGA TTTATTTACT
    GTGATACACA GGTCCAAAAG AAAGCTACTG GGCTGGAAAG AGACTGGGGA GGGCTTCACG
    GGAAGCAAAC CCAGCTCCCA TTCACCAGTG AAGAATACAG CTGATTCTCC CACCGGTGAG
    GCTGCTGCTG CCCCAGGGCC AAGTAGCAGT GCCTGCCTAG ATGCTGGTAG AAATGATGAT
    TTCAAGGCCT TGCTCCAGAA GAAGGGAAGT AAGGCAACTC CCAGGACCCG CCCCTCAGCG
    GCCGAGCTGC TGAAGACCAC TAACCCTCTG GCTCGAAGGA TTATTGCACA GTTCTCAAAA
    GACTACGAAC CCACTGACAA CCCCAGTACC TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu56084
    Clone ID Related Accession (Same CDS sequence) XM_006527725.3 , XM_006527725.4
    Accession Version XM_006527725.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2946bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product NHS-like protein 2 isoform X13
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000086.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006527725.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGGAGAGAG GCGAGTCTCT TACCCTCTTC TGGAGCCGGG GAGGGCAGCA GTTTGATAGA 
    CATGCAAGTT TTCGACACTC GTTGTTTAAC ACAGAGACAG CCGTGAACCC CAAGTCCACC
    CTGAGGCGGA GGCGGACCAT TATTGGATTC TCTAACTTTT CTCAGCGAGA CCAAGGTCAC
    AGCAGCAGCC CCACAGGCAG CCTGGCCCGC TCTGCCACCT CAGACATCAG GCCTGGCCAC
    TCAGCTCCAC AAGTTGTTCA GGGAAGAGTT GCTGTTGGGC AGGAAGCTCG GTTCCCAAGT
    CTCACCTCAC CAGGACTGAG ACACTCTTCT AGTGAGCCCG GAGACGCTCA CCAGGCACGC
    AGTGCCTCAG ACCATCCCGG CATGGAGAGC ATGGCAGTGG TGTACAGTGT CCCCAGTTCT
    TGCAATGGAC CAACAGAGTC AACATTCTCC ACTTCCTGGA AGGGAGATGC TTTCACATAC
    ATGACCCCAA GTGCCAGCAG CCAGGGCAAT CAAGTCAGTG AAAATGGAAA AAACCCTTCC
    TCGGGCAATT CTTGGGTCCC TCTGAACACA CTCCCACCTC TGGTTCCTAA GGAGGCGGCC
    ACGCTCTTTG TCACCCGTGA TAACCCAGCA GGATGCACTG GGCTACCCAG CTACTCTGAG
    CACCCCACTC TACGAAGACA GATACCAGAA AGACCTCCCA AGATTGGCCT TCTTGCCCGT
    GGTACCTCAA GGCTGGAAAC AGGCCCAGGT GGGACCAACA GGTTCCGGGA GCGGTCACTG
    TCTGTACCCA CAGACTCGGG TGTTACCTCA GTGGACTATG ACGAAGAACA GAAGACCAGT
    GAGACCCGCA TCCTGCCTTA TGCCAGTACA AGCTCCGAGG GCAGTAACAG TACTGACAAC
    ATTGCAGCCC TCAGCACTGA GCAGGAGGCA CGGCACAGAA GGCAAAGATC CAAAAGTATT
    TCACTCAAGA AGGCCAAAAA GAAGCCCTCT CCACCCATGC GAAGTGTCTC ACTCGTCAAA
    GATGAGCCAG CCCTCCCACC AGAAGGCGAA TTGGTACTGC CCAAGGACCA GAGGCCTAGG
    AGCCTTTGCC TCTCCTTGGA ACACCAAGGG CACCACCCAC CTCACCCAGA TGCTCAAGGT
    CACCCAGCTG TGCCAATGCT CAAAGATCCA GGAAGTACGC AGTTCTCTCA CCACTGGTAT
    CTTACTGACT GGAAGTCTGG TGACACCTAC CAGTCCTTGT CCAGCTCCAG CACTGCCACC
    GGCACCACGG TCATTGAGTG CACTCAAGTT CAGGGAAGCT CAGAGTCTCT GGCCTCCCCT
    TCCACCTCCA GAGCGACAAC ACCCTCCCAG CTCTCCATTG AGGTGGAAGC CAGGGAAGTA
    GCCTCTCCTG GGAGGCCCAC TGGACTGATG TCCCCTTCCA GTGGTTACTC CAGCCAGTCA
    GAGACACCCA CACCCACTGT CTCCATGTCC TTGACCCTGG GCCACTTACC TCCTCCAAGT
    GCTAGTGTCA GAGTACGTCC AGTGGTACCA GAAAGGAAGT CATCACTGCC CCCAACATCA
    CCAATGGAGA AAATCTGCAA GTCACGGTTA TCATTTGACC TACCACTGAC CTCTTCAACC
    ACCCTGGATC TGTCGGGGAT GAGTATCTCC ATCCGCAGCA AAACCAAGGT GAGCCGCCAT
    CACTCTGATA CCAATTTTGG GGTCAAACTG GCACAGAAAA CTAGTCCAAA CCAACCGATC
    ATGCCCATGG TTACTCAGTC TGACCTCCGT TCTGTTCGCC TGAGGTCAGT CAGCAAGTCT
    GAGCCAGAAG ACGACATTGA GAGTCCAGAT TACATAGAGG AACCTGGAGC AGAGGAAGTC
    TTCACCATGC CAGAGAGAAA AGTGAAACCT CCCATAGCCG AGAAACCTCC ATTGGCCCGA
    AGGCCTCCAA GTTTGGTCCA CAGGCCGCCC TCTCTCCCTG GGGAGTATCC ACTAACTTCT
    CCTACTATGG CTATGGCATC CAGGAGCTCC ATTCCACACA TGAAGCAGCT TCCCCAAGAC
    AGCTACACAG TGTTGCGGAA ACCAAAGTCA CCCAGCTTCC CCGGGGAGTC AACAGCATCC
    TCCAGCCTTG TCCTCTCACC TCTTGCCAGT TCCTCTGGTG CTTTCTTCTC AGGAACACAG
    CAACCTCCCC AGGCCAGTGT AGAAGACGGG GGCCCCAAGG TGAGAGCCCT GCCTGAAAGA
    ATCGGCCTCC AGAGCCAGGA AGAAGCTGAG AAAAAGATGA CCAAGATTCC ACCTCCGGTA
    CCAAAAAAGC CCAGTGTGCT CTACCTGCCT CTCACCTCCC CTGTAGCTCA AATGGATGCC
    TGCATGGCAG AACCAAGGCT ACCTTTCAGC CCCATCATCA CCCTGGAGGA AGATGGCAAG
    TGTCCCTCCA CTGGTGATGA CCAGAAGTCA CCTGGTAAAG GGGTGACTTC GCCTCTTCAC
    ACTGACACAG AAAAAGAGGC CATCTCTCCA GGGAGGTCAG TGGAACCGAG TGCTGAAGAG
    AAAAGTTTAA TCAGTGATAA AACAGCCGAA TGGATTGCAG AGGAGGAGGA TGACGTGTTT
    GTGGCTTCCC GCACAACTGA AGATTTATTT ACTGTGATAC ACAGGTCCAA AAGAAAGCTA
    CTGGGCTGGA AAGAGACTGG GGAGGGCTTC ACGGGAAGCA AACCCAGCTC CCATTCACCA
    GTGAAGAATA CAGCTGATTC TCCCACCGGT GAGGCTGCTG CTGCCCCAGG GCCAAGTAGC
    AGTGCCTGCC TAGATGCTGG TAGAAATGAT GATTTCAAGG CCTTGCTCCA GAAGAAGGGA
    AGTAAGGCAA CTCCCAGGAC CCGCCCCTCA GCGGCCGAGC TGCTGAAGAC CACTAACCCT
    CTGGCTCGAA GGATTATTGC ACAGTTCTCA AAAGACTACG AACCCACTGA CAACCCCAGT
    ACCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006527788.1
    CDS5960..8905
    Translation

    Target ORF information:

    RefSeq Version XM_006527725.4
    Organism Mus musculus(house mouse)
    Definition Mus musculus NHS-like 2 (Nhsl2), transcript variant X13, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006527725.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGGAGAGAG GCGAGTCTCT TACCCTCTTC TGGAGCCGGG GAGGGCAGCA GTTTGATAGA 
    CATGCAAGTT TTCGACACTC GTTGTTTAAC ACAGAGACAG CCGTGAACCC CAAGTCCACC
    CTGAGGCGGA GGCGGACCAT TATTGGATTC TCTAACTTTT CTCAGCGAGA CCAAGGTCAC
    AGCAGCAGCC CCACAGGCAG CCTGGCCCGC TCTGCCACCT CAGACATCAG GCCTGGCCAC
    TCAGCTCCAC AAGTTGTTCA GGGAAGAGTT GCTGTTGGGC AGGAAGCTCG GTTCCCAAGT
    CTCACCTCAC CAGGACTGAG ACACTCTTCT AGTGAGCCCG GAGACGCTCA CCAGGCACGC
    AGTGCCTCAG ACCATCCCGG CATGGAGAGC ATGGCAGTGG TGTACAGTGT CCCCAGTTCT
    TGCAATGGAC CAACAGAGTC AACATTCTCC ACTTCCTGGA AGGGAGATGC TTTCACATAC
    ATGACCCCAA GTGCCAGCAG CCAGGGCAAT CAAGTCAGTG AAAATGGAAA AAACCCTTCC
    TCGGGCAATT CTTGGGTCCC TCTGAACACA CTCCCACCTC TGGTTCCTAA GGAGGCGGCC
    ACGCTCTTTG TCACCCGTGA TAACCCAGCA GGATGCACTG GGCTACCCAG CTACTCTGAG
    CACCCCACTC TACGAAGACA GATACCAGAA AGACCTCCCA AGATTGGCCT TCTTGCCCGT
    GGTACCTCAA GGCTGGAAAC AGGCCCAGGT GGGACCAACA GGTTCCGGGA GCGGTCACTG
    TCTGTACCCA CAGACTCGGG TGTTACCTCA GTGGACTATG ACGAAGAACA GAAGACCAGT
    GAGACCCGCA TCCTGCCTTA TGCCAGTACA AGCTCCGAGG GCAGTAACAG TACTGACAAC
    ATTGCAGCCC TCAGCACTGA GCAGGAGGCA CGGCACAGAA GGCAAAGATC CAAAAGTATT
    TCACTCAAGA AGGCCAAAAA GAAGCCCTCT CCACCCATGC GAAGTGTCTC ACTCGTCAAA
    GATGAGCCAG CCCTCCCACC AGAAGGCGAA TTGGTACTGC CCAAGGACCA GAGGCCTAGG
    AGCCTTTGCC TCTCCTTGGA ACACCAAGGG CACCACCCAC CTCACCCAGA TGCTCAAGGT
    CACCCAGCTG TGCCAATGCT CAAAGATCCA GGAAGTACGC AGTTCTCTCA CCACTGGTAT
    CTTACTGACT GGAAGTCTGG TGACACCTAC CAGTCCTTGT CCAGCTCCAG CACTGCCACC
    GGCACCACGG TCATTGAGTG CACTCAAGTT CAGGGAAGCT CAGAGTCTCT GGCCTCCCCT
    TCCACCTCCA GAGCGACAAC ACCCTCCCAG CTCTCCATTG AGGTGGAAGC CAGGGAAGTA
    GCCTCTCCTG GGAGGCCCAC TGGACTGATG TCCCCTTCCA GTGGTTACTC CAGCCAGTCA
    GAGACACCCA CACCCACTGT CTCCATGTCC TTGACCCTGG GCCACTTACC TCCTCCAAGT
    GCTAGTGTCA GAGTACGTCC AGTGGTACCA GAAAGGAAGT CATCACTGCC CCCAACATCA
    CCAATGGAGA AAATCTGCAA GTCACGGTTA TCATTTGACC TACCACTGAC CTCTTCAACC
    ACCCTGGATC TGTCGGGGAT GAGTATCTCC ATCCGCAGCA AAACCAAGGT GAGCCGCCAT
    CACTCTGATA CCAATTTTGG GGTCAAACTG GCACAGAAAA CTAGTCCAAA CCAACCGATC
    ATGCCCATGG TTACTCAGTC TGACCTCCGT TCTGTTCGCC TGAGGTCAGT CAGCAAGTCT
    GAGCCAGAAG ACGACATTGA GAGTCCAGAT TACATAGAGG AACCTGGAGC AGAGGAAGTC
    TTCACCATGC CAGAGAGAAA AGTGAAACCT CCCATAGCCG AGAAACCTCC ATTGGCCCGA
    AGGCCTCCAA GTTTGGTCCA CAGGCCGCCC TCTCTCCCTG GGGAGTATCC ACTAACTTCT
    CCTACTATGG CTATGGCATC CAGGAGCTCC ATTCCACACA TGAAGCAGCT TCCCCAAGAC
    AGCTACACAG TGTTGCGGAA ACCAAAGTCA CCCAGCTTCC CCGGGGAGTC AACAGCATCC
    TCCAGCCTTG TCCTCTCACC TCTTGCCAGT TCCTCTGGTG CTTTCTTCTC AGGAACACAG
    CAACCTCCCC AGGCCAGTGT AGAAGACGGG GGCCCCAAGG TGAGAGCCCT GCCTGAAAGA
    ATCGGCCTCC AGAGCCAGGA AGAAGCTGAG AAAAAGATGA CCAAGATTCC ACCTCCGGTA
    CCAAAAAAGC CCAGTGTGCT CTACCTGCCT CTCACCTCCC CTGTAGCTCA AATGGATGCC
    TGCATGGCAG AACCAAGGCT ACCTTTCAGC CCCATCATCA CCCTGGAGGA AGATGGCAAG
    TGTCCCTCCA CTGGTGATGA CCAGAAGTCA CCTGGTAAAG GGGTGACTTC GCCTCTTCAC
    ACTGACACAG AAAAAGAGGC CATCTCTCCA GGGAGGTCAG TGGAACCGAG TGCTGAAGAG
    AAAAGTTTAA TCAGTGATAA AACAGCCGAA TGGATTGCAG AGGAGGAGGA TGACGTGTTT
    GTGGCTTCCC GCACAACTGA AGATTTATTT ACTGTGATAC ACAGGTCCAA AAGAAAGCTA
    CTGGGCTGGA AAGAGACTGG GGAGGGCTTC ACGGGAAGCA AACCCAGCTC CCATTCACCA
    GTGAAGAATA CAGCTGATTC TCCCACCGGT GAGGCTGCTG CTGCCCCAGG GCCAAGTAGC
    AGTGCCTGCC TAGATGCTGG TAGAAATGAT GATTTCAAGG CCTTGCTCCA GAAGAAGGGA
    AGTAAGGCAA CTCCCAGGAC CCGCCCCTCA GCGGCCGAGC TGCTGAAGAC CACTAACCCT
    CTGGCTCGAA GGATTATTGC ACAGTTCTCA AAAGACTACG AACCCACTGA CAACCCCAGT
    ACCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu56085
    Clone ID Related Accession (Same CDS sequence) XM_006527726.4 , XM_006527726.3
    Accession Version XM_006527726.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2931bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product NHS-like protein 2 isoform X14
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000086.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006527726.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    ATGGCAGCAC AGTCTGGAGC TTGGAATGGG CAGCAGTTTG ATAGACATGC AAGTTTTCGA 
    CACTCGTTGT TTAACACAGA GACAGCCGTG AACCCCAAGT CCACCCTGAG GCGGAGGCGG
    ACCATTATTG GATTCTCTAA CTTTTCTCAG CGAGACCAAG GTCACAGCAG CAGCCCCACA
    GGCAGCCTGG CCCGCTCTGC CACCTCAGAC ATCAGGCCTG GCCACTCAGC TCCACAAGTT
    GTTCAGGGAA GAGTTGCTGT TGGGCAGGAA GCTCGGTTCC CAAGTCTCAC CTCACCAGGA
    CTGAGACACT CTTCTAGTGA GCCCGGAGAC GCTCACCAGG CACGCAGTGC CTCAGACCAT
    CCCGGCATGG AGAGCATGGC AGTGGTGTAC AGTGTCCCCA GTTCTTGCAA TGGACCAACA
    GAGTCAACAT TCTCCACTTC CTGGAAGGGA GATGCTTTCA CATACATGAC CCCAAGTGCC
    AGCAGCCAGG GCAATCAAGT CAGTGAAAAT GGAAAAAACC CTTCCTCGGG CAATTCTTGG
    GTCCCTCTGA ACACACTCCC ACCTCTGGTT CCTAAGGAGG CGGCCACGCT CTTTGTCACC
    CGTGATAACC CAGCAGGATG CACTGGGCTA CCCAGCTACT CTGAGCACCC CACTCTACGA
    AGACAGATAC CAGAAAGACC TCCCAAGATT GGCCTTCTTG CCCGTGGTAC CTCAAGGCTG
    GAAACAGGCC CAGGTGGGAC CAACAGGTTC CGGGAGCGGT CACTGTCTGT ACCCACAGAC
    TCGGGTGTTA CCTCAGTGGA CTATGACGAA GAACAGAAGA CCAGTGAGAC CCGCATCCTG
    CCTTATGCCA GTACAAGCTC CGAGGGCAGT AACAGTACTG ACAACATTGC AGCCCTCAGC
    ACTGAGCAGG AGGCACGGCA CAGAAGGCAA AGATCCAAAA GTATTTCACT CAAGAAGGCC
    AAAAAGAAGC CCTCTCCACC CATGCGAAGT GTCTCACTCG TCAAAGATGA GCCAGCCCTC
    CCACCAGAAG GCGAATTGGT ACTGCCCAAG GACCAGAGGC CTAGGAGCCT TTGCCTCTCC
    TTGGAACACC AAGGGCACCA CCCACCTCAC CCAGATGCTC AAGGTCACCC AGCTGTGCCA
    ATGCTCAAAG ATCCAGGAAG TACGCAGTTC TCTCACCACT GGTATCTTAC TGACTGGAAG
    TCTGGTGACA CCTACCAGTC CTTGTCCAGC TCCAGCACTG CCACCGGCAC CACGGTCATT
    GAGTGCACTC AAGTTCAGGG AAGCTCAGAG TCTCTGGCCT CCCCTTCCAC CTCCAGAGCG
    ACAACACCCT CCCAGCTCTC CATTGAGGTG GAAGCCAGGG AAGTAGCCTC TCCTGGGAGG
    CCCACTGGAC TGATGTCCCC TTCCAGTGGT TACTCCAGCC AGTCAGAGAC ACCCACACCC
    ACTGTCTCCA TGTCCTTGAC CCTGGGCCAC TTACCTCCTC CAAGTGCTAG TGTCAGAGTA
    CGTCCAGTGG TACCAGAAAG GAAGTCATCA CTGCCCCCAA CATCACCAAT GGAGAAAATC
    TGCAAGTCAC GGTTATCATT TGACCTACCA CTGACCTCTT CAACCACCCT GGATCTGTCG
    GGGATGAGTA TCTCCATCCG CAGCAAAACC AAGGTGAGCC GCCATCACTC TGATACCAAT
    TTTGGGGTCA AACTGGCACA GAAAACTAGT CCAAACCAAC CGATCATGCC CATGGTTACT
    CAGTCTGACC TCCGTTCTGT TCGCCTGAGG TCAGTCAGCA AGTCTGAGCC AGAAGACGAC
    ATTGAGAGTC CAGATTACAT AGAGGAACCT GGAGCAGAGG AAGTCTTCAC CATGCCAGAG
    AGAAAAGTGA AACCTCCCAT AGCCGAGAAA CCTCCATTGG CCCGAAGGCC TCCAAGTTTG
    GTCCACAGGC CGCCCTCTCT CCCTGGGGAG TATCCACTAA CTTCTCCTAC TATGGCTATG
    GCATCCAGGA GCTCCATTCC ACACATGAAG CAGCTTCCCC AAGACAGCTA CACAGTGTTG
    CGGAAACCAA AGTCACCCAG CTTCCCCGGG GAGTCAACAG CATCCTCCAG CCTTGTCCTC
    TCACCTCTTG CCAGTTCCTC TGGTGCTTTC TTCTCAGGAA CACAGCAACC TCCCCAGGCC
    AGTGTAGAAG ACGGGGGCCC CAAGGTGAGA GCCCTGCCTG AAAGAATCGG CCTCCAGAGC
    CAGGAAGAAG CTGAGAAAAA GATGACCAAG ATTCCACCTC CGGTACCAAA AAAGCCCAGT
    GTGCTCTACC TGCCTCTCAC CTCCCCTGTA GCTCAAATGG ATGCCTGCAT GGCAGAACCA
    AGGCTACCTT TCAGCCCCAT CATCACCCTG GAGGAAGATG GCAAGTGTCC CTCCACTGGT
    GATGACCAGA AGTCACCTGG TAAAGGGGTG ACTTCGCCTC TTCACACTGA CACAGAAAAA
    GAGGCCATCT CTCCAGGGAG GTCAGTGGAA CCGAGTGCTG AAGAGAAAAG TTTAATCAGT
    GATAAAACAG CCGAATGGAT TGCAGAGGAG GAGGATGACG TGTTTGTGGC TTCCCGCACA
    ACTGAAGATT TATTTACTGT GATACACAGG TCCAAAAGAA AGCTACTGGG CTGGAAAGAG
    ACTGGGGAGG GCTTCACGGG AAGCAAACCC AGCTCCCATT CACCAGTGAA GAATACAGCT
    GATTCTCCCA CCGGTGAGGC TGCTGCTGCC CCAGGGCCAA GTAGCAGTGC CTGCCTAGAT
    GCTGGTAGAA ATGATGATTT CAAGGCCTTG CTCCAGAAGA AGGGAAGTAA GGCAACTCCC
    AGGACCCGCC CCTCAGCGGC CGAGCTGCTG AAGACCACTA ACCCTCTGGC TCGAAGGATT
    ATTGCACAGT TCTCAAAAGA CTACGAACCC ACTGACAACC CCAGTACCTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006527789.1
    CDS146..3076
    Translation

    Target ORF information:

    RefSeq Version XM_006527726.4
    Organism Mus musculus(house mouse)
    Definition Mus musculus NHS-like 2 (Nhsl2), transcript variant X14, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006527726.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    ATGGCAGCAC AGTCTGGAGC TTGGAATGGG CAGCAGTTTG ATAGACATGC AAGTTTTCGA 
    CACTCGTTGT TTAACACAGA GACAGCCGTG AACCCCAAGT CCACCCTGAG GCGGAGGCGG
    ACCATTATTG GATTCTCTAA CTTTTCTCAG CGAGACCAAG GTCACAGCAG CAGCCCCACA
    GGCAGCCTGG CCCGCTCTGC CACCTCAGAC ATCAGGCCTG GCCACTCAGC TCCACAAGTT
    GTTCAGGGAA GAGTTGCTGT TGGGCAGGAA GCTCGGTTCC CAAGTCTCAC CTCACCAGGA
    CTGAGACACT CTTCTAGTGA GCCCGGAGAC GCTCACCAGG CACGCAGTGC CTCAGACCAT
    CCCGGCATGG AGAGCATGGC AGTGGTGTAC AGTGTCCCCA GTTCTTGCAA TGGACCAACA
    GAGTCAACAT TCTCCACTTC CTGGAAGGGA GATGCTTTCA CATACATGAC CCCAAGTGCC
    AGCAGCCAGG GCAATCAAGT CAGTGAAAAT GGAAAAAACC CTTCCTCGGG CAATTCTTGG
    GTCCCTCTGA ACACACTCCC ACCTCTGGTT CCTAAGGAGG CGGCCACGCT CTTTGTCACC
    CGTGATAACC CAGCAGGATG CACTGGGCTA CCCAGCTACT CTGAGCACCC CACTCTACGA
    AGACAGATAC CAGAAAGACC TCCCAAGATT GGCCTTCTTG CCCGTGGTAC CTCAAGGCTG
    GAAACAGGCC CAGGTGGGAC CAACAGGTTC CGGGAGCGGT CACTGTCTGT ACCCACAGAC
    TCGGGTGTTA CCTCAGTGGA CTATGACGAA GAACAGAAGA CCAGTGAGAC CCGCATCCTG
    CCTTATGCCA GTACAAGCTC CGAGGGCAGT AACAGTACTG ACAACATTGC AGCCCTCAGC
    ACTGAGCAGG AGGCACGGCA CAGAAGGCAA AGATCCAAAA GTATTTCACT CAAGAAGGCC
    AAAAAGAAGC CCTCTCCACC CATGCGAAGT GTCTCACTCG TCAAAGATGA GCCAGCCCTC
    CCACCAGAAG GCGAATTGGT ACTGCCCAAG GACCAGAGGC CTAGGAGCCT TTGCCTCTCC
    TTGGAACACC AAGGGCACCA CCCACCTCAC CCAGATGCTC AAGGTCACCC AGCTGTGCCA
    ATGCTCAAAG ATCCAGGAAG TACGCAGTTC TCTCACCACT GGTATCTTAC TGACTGGAAG
    TCTGGTGACA CCTACCAGTC CTTGTCCAGC TCCAGCACTG CCACCGGCAC CACGGTCATT
    GAGTGCACTC AAGTTCAGGG AAGCTCAGAG TCTCTGGCCT CCCCTTCCAC CTCCAGAGCG
    ACAACACCCT CCCAGCTCTC CATTGAGGTG GAAGCCAGGG AAGTAGCCTC TCCTGGGAGG
    CCCACTGGAC TGATGTCCCC TTCCAGTGGT TACTCCAGCC AGTCAGAGAC ACCCACACCC
    ACTGTCTCCA TGTCCTTGAC CCTGGGCCAC TTACCTCCTC CAAGTGCTAG TGTCAGAGTA
    CGTCCAGTGG TACCAGAAAG GAAGTCATCA CTGCCCCCAA CATCACCAAT GGAGAAAATC
    TGCAAGTCAC GGTTATCATT TGACCTACCA CTGACCTCTT CAACCACCCT GGATCTGTCG
    GGGATGAGTA TCTCCATCCG CAGCAAAACC AAGGTGAGCC GCCATCACTC TGATACCAAT
    TTTGGGGTCA AACTGGCACA GAAAACTAGT CCAAACCAAC CGATCATGCC CATGGTTACT
    CAGTCTGACC TCCGTTCTGT TCGCCTGAGG TCAGTCAGCA AGTCTGAGCC AGAAGACGAC
    ATTGAGAGTC CAGATTACAT AGAGGAACCT GGAGCAGAGG AAGTCTTCAC CATGCCAGAG
    AGAAAAGTGA AACCTCCCAT AGCCGAGAAA CCTCCATTGG CCCGAAGGCC TCCAAGTTTG
    GTCCACAGGC CGCCCTCTCT CCCTGGGGAG TATCCACTAA CTTCTCCTAC TATGGCTATG
    GCATCCAGGA GCTCCATTCC ACACATGAAG CAGCTTCCCC AAGACAGCTA CACAGTGTTG
    CGGAAACCAA AGTCACCCAG CTTCCCCGGG GAGTCAACAG CATCCTCCAG CCTTGTCCTC
    TCACCTCTTG CCAGTTCCTC TGGTGCTTTC TTCTCAGGAA CACAGCAACC TCCCCAGGCC
    AGTGTAGAAG ACGGGGGCCC CAAGGTGAGA GCCCTGCCTG AAAGAATCGG CCTCCAGAGC
    CAGGAAGAAG CTGAGAAAAA GATGACCAAG ATTCCACCTC CGGTACCAAA AAAGCCCAGT
    GTGCTCTACC TGCCTCTCAC CTCCCCTGTA GCTCAAATGG ATGCCTGCAT GGCAGAACCA
    AGGCTACCTT TCAGCCCCAT CATCACCCTG GAGGAAGATG GCAAGTGTCC CTCCACTGGT
    GATGACCAGA AGTCACCTGG TAAAGGGGTG ACTTCGCCTC TTCACACTGA CACAGAAAAA
    GAGGCCATCT CTCCAGGGAG GTCAGTGGAA CCGAGTGCTG AAGAGAAAAG TTTAATCAGT
    GATAAAACAG CCGAATGGAT TGCAGAGGAG GAGGATGACG TGTTTGTGGC TTCCCGCACA
    ACTGAAGATT TATTTACTGT GATACACAGG TCCAAAAGAA AGCTACTGGG CTGGAAAGAG
    ACTGGGGAGG GCTTCACGGG AAGCAAACCC AGCTCCCATT CACCAGTGAA GAATACAGCT
    GATTCTCCCA CCGGTGAGGC TGCTGCTGCC CCAGGGCCAA GTAGCAGTGC CTGCCTAGAT
    GCTGGTAGAA ATGATGATTT CAAGGCCTTG CTCCAGAAGA AGGGAAGTAA GGCAACTCCC
    AGGACCCGCC CCTCAGCGGC CGAGCTGCTG AAGACCACTA ACCCTCTGGC TCGAAGGATT
    ATTGCACAGT TCTCAAAAGA CTACGAACCC ACTGACAACC CCAGTACCTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu56086
    Clone ID Related Accession (Same CDS sequence) XM_006527727.3 , XM_006527727.4
    Accession Version XM_006527727.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2841bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product NHS-like protein 2 isoform X15
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000086.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006527727.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    ATGATGGGCA ACTCTCACCA CAAGCAACCG AGGAGTAAGA GCCAAAGCAG CAGGATGCAT 
    TCAGCAACAG GTCACAGCAG CAGCCCCACA GGCAGCCTGG CCCGCTCTGC CACCTCAGAC
    ATCAGGCCTG GCCACTCAGC TCCACAAGTT GTTCAGGGAA GAGTTGCTGT TGGGCAGGAA
    GCTCGGTTCC CAAGTCTCAC CTCACCAGGA CTGAGACACT CTTCTAGTGA GCCCGGAGAC
    GCTCACCAGG CACGCAGTGC CTCAGACCAT CCCGGCATGG AGAGCATGGC AGTGGTGTAC
    AGTGTCCCCA GTTCTTGCAA TGGACCAACA GAGTCAACAT TCTCCACTTC CTGGAAGGGA
    GATGCTTTCA CATACATGAC CCCAAGTGCC AGCAGCCAGG GCAATCAAGT CAGTGAAAAT
    GGAAAAAACC CTTCCTCGGG CAATTCTTGG GTCCCTCTGA ACACACTCCC ACCTCTGGTT
    CCTAAGGAGG CGGCCACGCT CTTTGTCACC CGTGATAACC CAGCAGGATG CACTGGGCTA
    CCCAGCTACT CTGAGCACCC CACTCTACGA AGACAGATAC CAGAAAGACC TCCCAAGATT
    GGCCTTCTTG CCCGTGGTAC CTCAAGGCTG GAAACAGGCC CAGGTGGGAC CAACAGGTTC
    CGGGAGCGGT CACTGTCTGT ACCCACAGAC TCGGGTGTTA CCTCAGTGGA CTATGACGAA
    GAACAGAAGA CCAGTGAGAC CCGCATCCTG CCTTATGCCA GTACAAGCTC CGAGGGCAGT
    AACAGTACTG ACAACATTGC AGCCCTCAGC ACTGAGCAGG AGGCACGGCA CAGAAGGCAA
    AGATCCAAAA GTATTTCACT CAAGAAGGCC AAAAAGAAGC CCTCTCCACC CATGCGAAGT
    GTCTCACTCG TCAAAGATGA GCCAGCCCTC CCACCAGAAG GCGAATTGGT ACTGCCCAAG
    GACCAGAGGC CTAGGAGCCT TTGCCTCTCC TTGGAACACC AAGGGCACCA CCCACCTCAC
    CCAGATGCTC AAGGTCACCC AGCTGTGCCA ATGCTCAAAG ATCCAGGAAG TACGCAGTTC
    TCTCACCACT GGTATCTTAC TGACTGGAAG TCTGGTGACA CCTACCAGTC CTTGTCCAGC
    TCCAGCACTG CCACCGGCAC CACGGTCATT GAGTGCACTC AAGTTCAGGG AAGCTCAGAG
    TCTCTGGCCT CCCCTTCCAC CTCCAGAGCG ACAACACCCT CCCAGCTCTC CATTGAGGTG
    GAAGCCAGGG AAGTAGCCTC TCCTGGGAGG CCCACTGGAC TGATGTCCCC TTCCAGTGGT
    TACTCCAGCC AGTCAGAGAC ACCCACACCC ACTGTCTCCA TGTCCTTGAC CCTGGGCCAC
    TTACCTCCTC CAAGTGCTAG TGTCAGAGTA CGTCCAGTGG TACCAGAAAG GAAGTCATCA
    CTGCCCCCAA CATCACCAAT GGAGAAAATC TGCAAGTCAC GGTTATCATT TGACCTACCA
    CTGACCTCTT CAACCACCCT GGATCTGTCG GGGATGAGTA TCTCCATCCG CAGCAAAACC
    AAGGTGAGCC GCCATCACTC TGATACCAAT TTTGGGGTCA AACTGGCACA GAAAACTAGT
    CCAAACCAAC CGATCATGCC CATGGTTACT CAGTCTGACC TCCGTTCTGT TCGCCTGAGG
    TCAGTCAGCA AGTCTGAGCC AGAAGACGAC ATTGAGAGTC CAGATTACAT AGAGGAACCT
    GGAGCAGAGG AAGTCTTCAC CATGCCAGAG AGAAAAGTGA AACCTCCCAT AGCCGAGAAA
    CCTCCATTGG CCCGAAGGCC TCCAAGTTTG GTCCACAGGC CGCCCTCTCT CCCTGGGGAG
    TATCCACTAA CTTCTCCTAC TATGGCTATG GCATCCAGGA GCTCCATTCC ACACATGAAG
    CAGCTTCCCC AAGACAGCTA CACAGTGTTG CGGAAACCAA AGTCACCCAG CTTCCCCGGG
    GAGTCAACAG CATCCTCCAG CCTTGTCCTC TCACCTCTTG CCAGTTCCTC TGGTGCTTTC
    TTCTCAGGAA CACAGCAACC TCCCCAGGCC AGTGTAGAAG ACGGGGGCCC CAAGGTGAGA
    GCCCTGCCTG AAAGAATCGG CCTCCAGAGC CAGGAAGAAG CTGAGAAAAA GATGACCAAG
    ATTCCACCTC CGGTACCAAA AAAGCCCAGT GTGCTCTACC TGCCTCTCAC CTCCCCTGTA
    GCTCAAATGG ATGCCTGCAT GGCAGAACCA AGGCTACCTT TCAGCCCCAT CATCACCCTG
    GAGGAAGATG GCAAGTGTCC CTCCACTGGT GATGACCAGA AGTCACCTGG TAAAGGGGTG
    ACTTCGCCTC TTCACACTGA CACAGAAAAA GAGGCCATCT CTCCAGGGAG GTCAGTGGAA
    CCGAGTGCTG AAGAGAAAAG TTTAATCAGT GATAAAACAG CCGAATGGAT TGCAGAGGAG
    GAGGATGACG TGTTTGTGGC TTCCCGCACA ACTGAAGATT TATTTACTGT GATACACAGG
    TCCAAAAGAA AGCTACTGGG CTGGAAAGAG ACTGGGGAGG GCTTCACGGG AAGCAAACCC
    AGCTCCCATT CACCAGTGAA GAATACAGCT GATTCTCCCA CCGGTGAGGC TGCTGCTGCC
    CCAGGGCCAA GTAGCAGTGC CTGCCTAGAT GCTGGTAGAA ATGATGATTT CAAGGCCTTG
    CTCCAGAAGA AGGGAAGTAA GGCAACTCCC AGGACCCGCC CCTCAGCGGC CGAGCTGCTG
    AAGACCACTA ACCCTCTGGC TCGAAGGATT ATTGCACAGT TCTCAAAAGA CTACGAACCC
    ACTGACAACC CCAGTACCTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006527790.1
    CDS9856..12696
    Translation

    Target ORF information:

    RefSeq Version XM_006527727.4
    Organism Mus musculus(house mouse)
    Definition Mus musculus NHS-like 2 (Nhsl2), transcript variant X15, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006527727.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    ATGATGGGCA ACTCTCACCA CAAGCAACCG AGGAGTAAGA GCCAAAGCAG CAGGATGCAT 
    TCAGCAACAG GTCACAGCAG CAGCCCCACA GGCAGCCTGG CCCGCTCTGC CACCTCAGAC
    ATCAGGCCTG GCCACTCAGC TCCACAAGTT GTTCAGGGAA GAGTTGCTGT TGGGCAGGAA
    GCTCGGTTCC CAAGTCTCAC CTCACCAGGA CTGAGACACT CTTCTAGTGA GCCCGGAGAC
    GCTCACCAGG CACGCAGTGC CTCAGACCAT CCCGGCATGG AGAGCATGGC AGTGGTGTAC
    AGTGTCCCCA GTTCTTGCAA TGGACCAACA GAGTCAACAT TCTCCACTTC CTGGAAGGGA
    GATGCTTTCA CATACATGAC CCCAAGTGCC AGCAGCCAGG GCAATCAAGT CAGTGAAAAT
    GGAAAAAACC CTTCCTCGGG CAATTCTTGG GTCCCTCTGA ACACACTCCC ACCTCTGGTT
    CCTAAGGAGG CGGCCACGCT CTTTGTCACC CGTGATAACC CAGCAGGATG CACTGGGCTA
    CCCAGCTACT CTGAGCACCC CACTCTACGA AGACAGATAC CAGAAAGACC TCCCAAGATT
    GGCCTTCTTG CCCGTGGTAC CTCAAGGCTG GAAACAGGCC CAGGTGGGAC CAACAGGTTC
    CGGGAGCGGT CACTGTCTGT ACCCACAGAC TCGGGTGTTA CCTCAGTGGA CTATGACGAA
    GAACAGAAGA CCAGTGAGAC CCGCATCCTG CCTTATGCCA GTACAAGCTC CGAGGGCAGT
    AACAGTACTG ACAACATTGC AGCCCTCAGC ACTGAGCAGG AGGCACGGCA CAGAAGGCAA
    AGATCCAAAA GTATTTCACT CAAGAAGGCC AAAAAGAAGC CCTCTCCACC CATGCGAAGT
    GTCTCACTCG TCAAAGATGA GCCAGCCCTC CCACCAGAAG GCGAATTGGT ACTGCCCAAG
    GACCAGAGGC CTAGGAGCCT TTGCCTCTCC TTGGAACACC AAGGGCACCA CCCACCTCAC
    CCAGATGCTC AAGGTCACCC AGCTGTGCCA ATGCTCAAAG ATCCAGGAAG TACGCAGTTC
    TCTCACCACT GGTATCTTAC TGACTGGAAG TCTGGTGACA CCTACCAGTC CTTGTCCAGC
    TCCAGCACTG CCACCGGCAC CACGGTCATT GAGTGCACTC AAGTTCAGGG AAGCTCAGAG
    TCTCTGGCCT CCCCTTCCAC CTCCAGAGCG ACAACACCCT CCCAGCTCTC CATTGAGGTG
    GAAGCCAGGG AAGTAGCCTC TCCTGGGAGG CCCACTGGAC TGATGTCCCC TTCCAGTGGT
    TACTCCAGCC AGTCAGAGAC ACCCACACCC ACTGTCTCCA TGTCCTTGAC CCTGGGCCAC
    TTACCTCCTC CAAGTGCTAG TGTCAGAGTA CGTCCAGTGG TACCAGAAAG GAAGTCATCA
    CTGCCCCCAA CATCACCAAT GGAGAAAATC TGCAAGTCAC GGTTATCATT TGACCTACCA
    CTGACCTCTT CAACCACCCT GGATCTGTCG GGGATGAGTA TCTCCATCCG CAGCAAAACC
    AAGGTGAGCC GCCATCACTC TGATACCAAT TTTGGGGTCA AACTGGCACA GAAAACTAGT
    CCAAACCAAC CGATCATGCC CATGGTTACT CAGTCTGACC TCCGTTCTGT TCGCCTGAGG
    TCAGTCAGCA AGTCTGAGCC AGAAGACGAC ATTGAGAGTC CAGATTACAT AGAGGAACCT
    GGAGCAGAGG AAGTCTTCAC CATGCCAGAG AGAAAAGTGA AACCTCCCAT AGCCGAGAAA
    CCTCCATTGG CCCGAAGGCC TCCAAGTTTG GTCCACAGGC CGCCCTCTCT CCCTGGGGAG
    TATCCACTAA CTTCTCCTAC TATGGCTATG GCATCCAGGA GCTCCATTCC ACACATGAAG
    CAGCTTCCCC AAGACAGCTA CACAGTGTTG CGGAAACCAA AGTCACCCAG CTTCCCCGGG
    GAGTCAACAG CATCCTCCAG CCTTGTCCTC TCACCTCTTG CCAGTTCCTC TGGTGCTTTC
    TTCTCAGGAA CACAGCAACC TCCCCAGGCC AGTGTAGAAG ACGGGGGCCC CAAGGTGAGA
    GCCCTGCCTG AAAGAATCGG CCTCCAGAGC CAGGAAGAAG CTGAGAAAAA GATGACCAAG
    ATTCCACCTC CGGTACCAAA AAAGCCCAGT GTGCTCTACC TGCCTCTCAC CTCCCCTGTA
    GCTCAAATGG ATGCCTGCAT GGCAGAACCA AGGCTACCTT TCAGCCCCAT CATCACCCTG
    GAGGAAGATG GCAAGTGTCC CTCCACTGGT GATGACCAGA AGTCACCTGG TAAAGGGGTG
    ACTTCGCCTC TTCACACTGA CACAGAAAAA GAGGCCATCT CTCCAGGGAG GTCAGTGGAA
    CCGAGTGCTG AAGAGAAAAG TTTAATCAGT GATAAAACAG CCGAATGGAT TGCAGAGGAG
    GAGGATGACG TGTTTGTGGC TTCCCGCACA ACTGAAGATT TATTTACTGT GATACACAGG
    TCCAAAAGAA AGCTACTGGG CTGGAAAGAG ACTGGGGAGG GCTTCACGGG AAGCAAACCC
    AGCTCCCATT CACCAGTGAA GAATACAGCT GATTCTCCCA CCGGTGAGGC TGCTGCTGCC
    CCAGGGCCAA GTAGCAGTGC CTGCCTAGAT GCTGGTAGAA ATGATGATTT CAAGGCCTTG
    CTCCAGAAGA AGGGAAGTAA GGCAACTCCC AGGACCCGCC CCTCAGCGGC CGAGCTGCTG
    AAGACCACTA ACCCTCTGGC TCGAAGGATT ATTGCACAGT TCTCAAAAGA CTACGAACCC
    ACTGACAACC CCAGTACCTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu56087
    Clone ID Related Accession (Same CDS sequence) XM_006527728.3 , XM_006527728.4
    Accession Version XM_006527728.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2799bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product NHS-like protein 2 isoform X16
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000086.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006527728.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    ATGGCAGCAC AGTCTGGAGC TTGGAATGGT CACAGCAGCA GCCCCACAGG CAGCCTGGCC 
    CGCTCTGCCA CCTCAGACAT CAGGCCTGGC CACTCAGCTC CACAAGTTGT TCAGGGAAGA
    GTTGCTGTTG GGCAGGAAGC TCGGTTCCCA AGTCTCACCT CACCAGGACT GAGACACTCT
    TCTAGTGAGC CCGGAGACGC TCACCAGGCA CGCAGTGCCT CAGACCATCC CGGCATGGAG
    AGCATGGCAG TGGTGTACAG TGTCCCCAGT TCTTGCAATG GACCAACAGA GTCAACATTC
    TCCACTTCCT GGAAGGGAGA TGCTTTCACA TACATGACCC CAAGTGCCAG CAGCCAGGGC
    AATCAAGTCA GTGAAAATGG AAAAAACCCT TCCTCGGGCA ATTCTTGGGT CCCTCTGAAC
    ACACTCCCAC CTCTGGTTCC TAAGGAGGCG GCCACGCTCT TTGTCACCCG TGATAACCCA
    GCAGGATGCA CTGGGCTACC CAGCTACTCT GAGCACCCCA CTCTACGAAG ACAGATACCA
    GAAAGACCTC CCAAGATTGG CCTTCTTGCC CGTGGTACCT CAAGGCTGGA AACAGGCCCA
    GGTGGGACCA ACAGGTTCCG GGAGCGGTCA CTGTCTGTAC CCACAGACTC GGGTGTTACC
    TCAGTGGACT ATGACGAAGA ACAGAAGACC AGTGAGACCC GCATCCTGCC TTATGCCAGT
    ACAAGCTCCG AGGGCAGTAA CAGTACTGAC AACATTGCAG CCCTCAGCAC TGAGCAGGAG
    GCACGGCACA GAAGGCAAAG ATCCAAAAGT ATTTCACTCA AGAAGGCCAA AAAGAAGCCC
    TCTCCACCCA TGCGAAGTGT CTCACTCGTC AAAGATGAGC CAGCCCTCCC ACCAGAAGGC
    GAATTGGTAC TGCCCAAGGA CCAGAGGCCT AGGAGCCTTT GCCTCTCCTT GGAACACCAA
    GGGCACCACC CACCTCACCC AGATGCTCAA GGTCACCCAG CTGTGCCAAT GCTCAAAGAT
    CCAGGAAGTA CGCAGTTCTC TCACCACTGG TATCTTACTG ACTGGAAGTC TGGTGACACC
    TACCAGTCCT TGTCCAGCTC CAGCACTGCC ACCGGCACCA CGGTCATTGA GTGCACTCAA
    GTTCAGGGAA GCTCAGAGTC TCTGGCCTCC CCTTCCACCT CCAGAGCGAC AACACCCTCC
    CAGCTCTCCA TTGAGGTGGA AGCCAGGGAA GTAGCCTCTC CTGGGAGGCC CACTGGACTG
    ATGTCCCCTT CCAGTGGTTA CTCCAGCCAG TCAGAGACAC CCACACCCAC TGTCTCCATG
    TCCTTGACCC TGGGCCACTT ACCTCCTCCA AGTGCTAGTG TCAGAGTACG TCCAGTGGTA
    CCAGAAAGGA AGTCATCACT GCCCCCAACA TCACCAATGG AGAAAATCTG CAAGTCACGG
    TTATCATTTG ACCTACCACT GACCTCTTCA ACCACCCTGG ATCTGTCGGG GATGAGTATC
    TCCATCCGCA GCAAAACCAA GGTGAGCCGC CATCACTCTG ATACCAATTT TGGGGTCAAA
    CTGGCACAGA AAACTAGTCC AAACCAACCG ATCATGCCCA TGGTTACTCA GTCTGACCTC
    CGTTCTGTTC GCCTGAGGTC AGTCAGCAAG TCTGAGCCAG AAGACGACAT TGAGAGTCCA
    GATTACATAG AGGAACCTGG AGCAGAGGAA GTCTTCACCA TGCCAGAGAG AAAAGTGAAA
    CCTCCCATAG CCGAGAAACC TCCATTGGCC CGAAGGCCTC CAAGTTTGGT CCACAGGCCG
    CCCTCTCTCC CTGGGGAGTA TCCACTAACT TCTCCTACTA TGGCTATGGC ATCCAGGAGC
    TCCATTCCAC ACATGAAGCA GCTTCCCCAA GACAGCTACA CAGTGTTGCG GAAACCAAAG
    TCACCCAGCT TCCCCGGGGA GTCAACAGCA TCCTCCAGCC TTGTCCTCTC ACCTCTTGCC
    AGTTCCTCTG GTGCTTTCTT CTCAGGAACA CAGCAACCTC CCCAGGCCAG TGTAGAAGAC
    GGGGGCCCCA AGGTGAGAGC CCTGCCTGAA AGAATCGGCC TCCAGAGCCA GGAAGAAGCT
    GAGAAAAAGA TGACCAAGAT TCCACCTCCG GTACCAAAAA AGCCCAGTGT GCTCTACCTG
    CCTCTCACCT CCCCTGTAGC TCAAATGGAT GCCTGCATGG CAGAACCAAG GCTACCTTTC
    AGCCCCATCA TCACCCTGGA GGAAGATGGC AAGTGTCCCT CCACTGGTGA TGACCAGAAG
    TCACCTGGTA AAGGGGTGAC TTCGCCTCTT CACACTGACA CAGAAAAAGA GGCCATCTCT
    CCAGGGAGGT CAGTGGAACC GAGTGCTGAA GAGAAAAGTT TAATCAGTGA TAAAACAGCC
    GAATGGATTG CAGAGGAGGA GGATGACGTG TTTGTGGCTT CCCGCACAAC TGAAGATTTA
    TTTACTGTGA TACACAGGTC CAAAAGAAAG CTACTGGGCT GGAAAGAGAC TGGGGAGGGC
    TTCACGGGAA GCAAACCCAG CTCCCATTCA CCAGTGAAGA ATACAGCTGA TTCTCCCACC
    GGTGAGGCTG CTGCTGCCCC AGGGCCAAGT AGCAGTGCCT GCCTAGATGC TGGTAGAAAT
    GATGATTTCA AGGCCTTGCT CCAGAAGAAG GGAAGTAAGG CAACTCCCAG GACCCGCCCC
    TCAGCGGCCG AGCTGCTGAA GACCACTAAC CCTCTGGCTC GAAGGATTAT TGCACAGTTC
    TCAAAAGACT ACGAACCCAC TGACAACCCC AGTACCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006527791.1
    CDS145..2943
    Translation

    Target ORF information:

    RefSeq Version XM_006527728.4
    Organism Mus musculus(house mouse)
    Definition Mus musculus NHS-like 2 (Nhsl2), transcript variant X16, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006527728.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    ATGGCAGCAC AGTCTGGAGC TTGGAATGGT CACAGCAGCA GCCCCACAGG CAGCCTGGCC 
    CGCTCTGCCA CCTCAGACAT CAGGCCTGGC CACTCAGCTC CACAAGTTGT TCAGGGAAGA
    GTTGCTGTTG GGCAGGAAGC TCGGTTCCCA AGTCTCACCT CACCAGGACT GAGACACTCT
    TCTAGTGAGC CCGGAGACGC TCACCAGGCA CGCAGTGCCT CAGACCATCC CGGCATGGAG
    AGCATGGCAG TGGTGTACAG TGTCCCCAGT TCTTGCAATG GACCAACAGA GTCAACATTC
    TCCACTTCCT GGAAGGGAGA TGCTTTCACA TACATGACCC CAAGTGCCAG CAGCCAGGGC
    AATCAAGTCA GTGAAAATGG AAAAAACCCT TCCTCGGGCA ATTCTTGGGT CCCTCTGAAC
    ACACTCCCAC CTCTGGTTCC TAAGGAGGCG GCCACGCTCT TTGTCACCCG TGATAACCCA
    GCAGGATGCA CTGGGCTACC CAGCTACTCT GAGCACCCCA CTCTACGAAG ACAGATACCA
    GAAAGACCTC CCAAGATTGG CCTTCTTGCC CGTGGTACCT CAAGGCTGGA AACAGGCCCA
    GGTGGGACCA ACAGGTTCCG GGAGCGGTCA CTGTCTGTAC CCACAGACTC GGGTGTTACC
    TCAGTGGACT ATGACGAAGA ACAGAAGACC AGTGAGACCC GCATCCTGCC TTATGCCAGT
    ACAAGCTCCG AGGGCAGTAA CAGTACTGAC AACATTGCAG CCCTCAGCAC TGAGCAGGAG
    GCACGGCACA GAAGGCAAAG ATCCAAAAGT ATTTCACTCA AGAAGGCCAA AAAGAAGCCC
    TCTCCACCCA TGCGAAGTGT CTCACTCGTC AAAGATGAGC CAGCCCTCCC ACCAGAAGGC
    GAATTGGTAC TGCCCAAGGA CCAGAGGCCT AGGAGCCTTT GCCTCTCCTT GGAACACCAA
    GGGCACCACC CACCTCACCC AGATGCTCAA GGTCACCCAG CTGTGCCAAT GCTCAAAGAT
    CCAGGAAGTA CGCAGTTCTC TCACCACTGG TATCTTACTG ACTGGAAGTC TGGTGACACC
    TACCAGTCCT TGTCCAGCTC CAGCACTGCC ACCGGCACCA CGGTCATTGA GTGCACTCAA
    GTTCAGGGAA GCTCAGAGTC TCTGGCCTCC CCTTCCACCT CCAGAGCGAC AACACCCTCC
    CAGCTCTCCA TTGAGGTGGA AGCCAGGGAA GTAGCCTCTC CTGGGAGGCC CACTGGACTG
    ATGTCCCCTT CCAGTGGTTA CTCCAGCCAG TCAGAGACAC CCACACCCAC TGTCTCCATG
    TCCTTGACCC TGGGCCACTT ACCTCCTCCA AGTGCTAGTG TCAGAGTACG TCCAGTGGTA
    CCAGAAAGGA AGTCATCACT GCCCCCAACA TCACCAATGG AGAAAATCTG CAAGTCACGG
    TTATCATTTG ACCTACCACT GACCTCTTCA ACCACCCTGG ATCTGTCGGG GATGAGTATC
    TCCATCCGCA GCAAAACCAA GGTGAGCCGC CATCACTCTG ATACCAATTT TGGGGTCAAA
    CTGGCACAGA AAACTAGTCC AAACCAACCG ATCATGCCCA TGGTTACTCA GTCTGACCTC
    CGTTCTGTTC GCCTGAGGTC AGTCAGCAAG TCTGAGCCAG AAGACGACAT TGAGAGTCCA
    GATTACATAG AGGAACCTGG AGCAGAGGAA GTCTTCACCA TGCCAGAGAG AAAAGTGAAA
    CCTCCCATAG CCGAGAAACC TCCATTGGCC CGAAGGCCTC CAAGTTTGGT CCACAGGCCG
    CCCTCTCTCC CTGGGGAGTA TCCACTAACT TCTCCTACTA TGGCTATGGC ATCCAGGAGC
    TCCATTCCAC ACATGAAGCA GCTTCCCCAA GACAGCTACA CAGTGTTGCG GAAACCAAAG
    TCACCCAGCT TCCCCGGGGA GTCAACAGCA TCCTCCAGCC TTGTCCTCTC ACCTCTTGCC
    AGTTCCTCTG GTGCTTTCTT CTCAGGAACA CAGCAACCTC CCCAGGCCAG TGTAGAAGAC
    GGGGGCCCCA AGGTGAGAGC CCTGCCTGAA AGAATCGGCC TCCAGAGCCA GGAAGAAGCT
    GAGAAAAAGA TGACCAAGAT TCCACCTCCG GTACCAAAAA AGCCCAGTGT GCTCTACCTG
    CCTCTCACCT CCCCTGTAGC TCAAATGGAT GCCTGCATGG CAGAACCAAG GCTACCTTTC
    AGCCCCATCA TCACCCTGGA GGAAGATGGC AAGTGTCCCT CCACTGGTGA TGACCAGAAG
    TCACCTGGTA AAGGGGTGAC TTCGCCTCTT CACACTGACA CAGAAAAAGA GGCCATCTCT
    CCAGGGAGGT CAGTGGAACC GAGTGCTGAA GAGAAAAGTT TAATCAGTGA TAAAACAGCC
    GAATGGATTG CAGAGGAGGA GGATGACGTG TTTGTGGCTT CCCGCACAAC TGAAGATTTA
    TTTACTGTGA TACACAGGTC CAAAAGAAAG CTACTGGGCT GGAAAGAGAC TGGGGAGGGC
    TTCACGGGAA GCAAACCCAG CTCCCATTCA CCAGTGAAGA ATACAGCTGA TTCTCCCACC
    GGTGAGGCTG CTGCTGCCCC AGGGCCAAGT AGCAGTGCCT GCCTAGATGC TGGTAGAAAT
    GATGATTTCA AGGCCTTGCT CCAGAAGAAG GGAAGTAAGG CAACTCCCAG GACCCGCCCC
    TCAGCGGCCG AGCTGCTGAA GACCACTAAC CCTCTGGCTC GAAGGATTAT TGCACAGTTC
    TCAAAAGACT ACGAACCCAC TGACAACCCC AGTACCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu56088
    Clone ID Related Accession (Same CDS sequence) XM_006527729.3 , XM_030251165.1 , XM_006527729.4
    Accession Version XM_030251165.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2565bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product NHS-like protein 2 isoform X17
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000086.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    ATGGAGAGCA TGGCAGTGGT GTACAGTGTC CCCAGTTCTT GCAATGGACC AACAGAGTCA 
    ACATTCTCCA CTTCCTGGAA GGGAGATGCT TTCACATACA TGACCCCAAG TGCCAGCAGC
    CAGGGCAATC AAGTCAGTGA AAATGGAAAA AACCCTTCCT CGGGCAATTC TTGGGTCCCT
    CTGAACACAC TCCCACCTCT GGTTCCTAAG GAGGCGGCCA CGCTCTTTGT CACCCGTGAT
    AACCCAGCAG GATGCACTGG GCTACCCAGC TACTCTGAGC ACCCCACTCT ACGAAGACAG
    ATACCAGAAA GACCTCCCAA GATTGGCCTT CTTGCCCGTG GTACCTCAAG GCTGGAAACA
    GGCCCAGGTG GGACCAACAG GTTCCGGGAG CGGTCACTGT CTGTACCCAC AGACTCGGGT
    GTTACCTCAG TGGACTATGA CGAAGAACAG AAGACCAGTG AGACCCGCAT CCTGCCTTAT
    GCCAGTACAA GCTCCGAGGG CAGTAACAGT ACTGACAACA TTGCAGCCCT CAGCACTGAG
    CAGGAGGCAC GGCACAGAAG GCAAAGATCC AAAAGTATTT CACTCAAGAA GGCCAAAAAG
    AAGCCCTCTC CACCCATGCG AAGTGTCTCA CTCGTCAAAG ATGAGCCAGC CCTCCCACCA
    GAAGGCGAAT TGGTACTGCC CAAGGACCAG AGGCCTAGGA GCCTTTGCCT CTCCTTGGAA
    CACCAAGGGC ACCACCCACC TCACCCAGAT GCTCAAGGTC ACCCAGCTGT GCCAATGCTC
    AAAGATCCAG GAAGTACGCA GTTCTCTCAC CACTGGTATC TTACTGACTG GAAGTCTGGT
    GACACCTACC AGTCCTTGTC CAGCTCCAGC ACTGCCACCG GCACCACGGT CATTGAGTGC
    ACTCAAGTTC AGGGAAGCTC AGAGTCTCTG GCCTCCCCTT CCACCTCCAG AGCGACAACA
    CCCTCCCAGC TCTCCATTGA GGTGGAAGCC AGGGAAGTAG CCTCTCCTGG GAGGCCCACT
    GGACTGATGT CCCCTTCCAG TGGTTACTCC AGCCAGTCAG AGACACCCAC ACCCACTGTC
    TCCATGTCCT TGACCCTGGG CCACTTACCT CCTCCAAGTG CTAGTGTCAG AGTACGTCCA
    GTGGTACCAG AAAGGAAGTC ATCACTGCCC CCAACATCAC CAATGGAGAA AATCTGCAAG
    TCACGGTTAT CATTTGACCT ACCACTGACC TCTTCAACCA CCCTGGATCT GTCGGGGATG
    AGTATCTCCA TCCGCAGCAA AACCAAGGTG AGCCGCCATC ACTCTGATAC CAATTTTGGG
    GTCAAACTGG CACAGAAAAC TAGTCCAAAC CAACCGATCA TGCCCATGGT TACTCAGTCT
    GACCTCCGTT CTGTTCGCCT GAGGTCAGTC AGCAAGTCTG AGCCAGAAGA CGACATTGAG
    AGTCCAGATT ACATAGAGGA ACCTGGAGCA GAGGAAGTCT TCACCATGCC AGAGAGAAAA
    GTGAAACCTC CCATAGCCGA GAAACCTCCA TTGGCCCGAA GGCCTCCAAG TTTGGTCCAC
    AGGCCGCCCT CTCTCCCTGG GGAGTATCCA CTAACTTCTC CTACTATGGC TATGGCATCC
    AGGAGCTCCA TTCCACACAT GAAGCAGCTT CCCCAAGACA GCTACACAGT GTTGCGGAAA
    CCAAAGTCAC CCAGCTTCCC CGGGGAGTCA ACAGCATCCT CCAGCCTTGT CCTCTCACCT
    CTTGCCAGTT CCTCTGGTGC TTTCTTCTCA GGAACACAGC AACCTCCCCA GGCCAGTGTA
    GAAGACGGGG GCCCCAAGGT GAGAGCCCTG CCTGAAAGAA TCGGCCTCCA GAGCCAGGAA
    GAAGCTGAGA AAAAGATGAC CAAGATTCCA CCTCCGGTAC CAAAAAAGCC CAGTGTGCTC
    TACCTGCCTC TCACCTCCCC TGTAGCTCAA ATGGATGCCT GCATGGCAGA ACCAAGGCTA
    CCTTTCAGCC CCATCATCAC CCTGGAGGAA GATGGCAAGT GTCCCTCCAC TGGTGATGAC
    CAGAAGTCAC CTGGTAAAGG GGTGACTTCG CCTCTTCACA CTGACACAGA AAAAGAGGCC
    ATCTCTCCAG GGAGGTCAGT GGAACCGAGT GCTGAAGAGA AAAGTTTAAT CAGTGATAAA
    ACAGCCGAAT GGATTGCAGA GGAGGAGGAT GACGTGTTTG TGGCTTCCCG CACAACTGAA
    GATTTATTTA CTGTGATACA CAGGTCCAAA AGAAAGCTAC TGGGCTGGAA AGAGACTGGG
    GAGGGCTTCA CGGGAAGCAA ACCCAGCTCC CATTCACCAG TGAAGAATAC AGCTGATTCT
    CCCACCGGTG AGGCTGCTGC TGCCCCAGGG CCAAGTAGCA GTGCCTGCCT AGATGCTGGT
    AGAAATGATG ATTTCAAGGC CTTGCTCCAG AAGAAGGGAA GTAAGGCAAC TCCCAGGACC
    CGCCCCTCAG CGGCCGAGCT GCTGAAGACC ACTAACCCTC TGGCTCGAAG GATTATTGCA
    CAGTTCTCAA AAGACTACGA ACCCACTGAC AACCCCAGTA CCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_030107025.1
    CDS2138..4702
    Translation

    Target ORF information:

    RefSeq Version XM_030251165.1
    Organism Mus musculus(house mouse)
    Definition Mus musculus NHS-like 2 (Nhsl2), transcript variant X17, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_030251165.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    ATGGAGAGCA TGGCAGTGGT GTACAGTGTC CCCAGTTCTT GCAATGGACC AACAGAGTCA 
    ACATTCTCCA CTTCCTGGAA GGGAGATGCT TTCACATACA TGACCCCAAG TGCCAGCAGC
    CAGGGCAATC AAGTCAGTGA AAATGGAAAA AACCCTTCCT CGGGCAATTC TTGGGTCCCT
    CTGAACACAC TCCCACCTCT GGTTCCTAAG GAGGCGGCCA CGCTCTTTGT CACCCGTGAT
    AACCCAGCAG GATGCACTGG GCTACCCAGC TACTCTGAGC ACCCCACTCT ACGAAGACAG
    ATACCAGAAA GACCTCCCAA GATTGGCCTT CTTGCCCGTG GTACCTCAAG GCTGGAAACA
    GGCCCAGGTG GGACCAACAG GTTCCGGGAG CGGTCACTGT CTGTACCCAC AGACTCGGGT
    GTTACCTCAG TGGACTATGA CGAAGAACAG AAGACCAGTG AGACCCGCAT CCTGCCTTAT
    GCCAGTACAA GCTCCGAGGG CAGTAACAGT ACTGACAACA TTGCAGCCCT CAGCACTGAG
    CAGGAGGCAC GGCACAGAAG GCAAAGATCC AAAAGTATTT CACTCAAGAA GGCCAAAAAG
    AAGCCCTCTC CACCCATGCG AAGTGTCTCA CTCGTCAAAG ATGAGCCAGC CCTCCCACCA
    GAAGGCGAAT TGGTACTGCC CAAGGACCAG AGGCCTAGGA GCCTTTGCCT CTCCTTGGAA
    CACCAAGGGC ACCACCCACC TCACCCAGAT GCTCAAGGTC ACCCAGCTGT GCCAATGCTC
    AAAGATCCAG GAAGTACGCA GTTCTCTCAC CACTGGTATC TTACTGACTG GAAGTCTGGT
    GACACCTACC AGTCCTTGTC CAGCTCCAGC ACTGCCACCG GCACCACGGT CATTGAGTGC
    ACTCAAGTTC AGGGAAGCTC AGAGTCTCTG GCCTCCCCTT CCACCTCCAG AGCGACAACA
    CCCTCCCAGC TCTCCATTGA GGTGGAAGCC AGGGAAGTAG CCTCTCCTGG GAGGCCCACT
    GGACTGATGT CCCCTTCCAG TGGTTACTCC AGCCAGTCAG AGACACCCAC ACCCACTGTC
    TCCATGTCCT TGACCCTGGG CCACTTACCT CCTCCAAGTG CTAGTGTCAG AGTACGTCCA
    GTGGTACCAG AAAGGAAGTC ATCACTGCCC CCAACATCAC CAATGGAGAA AATCTGCAAG
    TCACGGTTAT CATTTGACCT ACCACTGACC TCTTCAACCA CCCTGGATCT GTCGGGGATG
    AGTATCTCCA TCCGCAGCAA AACCAAGGTG AGCCGCCATC ACTCTGATAC CAATTTTGGG
    GTCAAACTGG CACAGAAAAC TAGTCCAAAC CAACCGATCA TGCCCATGGT TACTCAGTCT
    GACCTCCGTT CTGTTCGCCT GAGGTCAGTC AGCAAGTCTG AGCCAGAAGA CGACATTGAG
    AGTCCAGATT ACATAGAGGA ACCTGGAGCA GAGGAAGTCT TCACCATGCC AGAGAGAAAA
    GTGAAACCTC CCATAGCCGA GAAACCTCCA TTGGCCCGAA GGCCTCCAAG TTTGGTCCAC
    AGGCCGCCCT CTCTCCCTGG GGAGTATCCA CTAACTTCTC CTACTATGGC TATGGCATCC
    AGGAGCTCCA TTCCACACAT GAAGCAGCTT CCCCAAGACA GCTACACAGT GTTGCGGAAA
    CCAAAGTCAC CCAGCTTCCC CGGGGAGTCA ACAGCATCCT CCAGCCTTGT CCTCTCACCT
    CTTGCCAGTT CCTCTGGTGC TTTCTTCTCA GGAACACAGC AACCTCCCCA GGCCAGTGTA
    GAAGACGGGG GCCCCAAGGT GAGAGCCCTG CCTGAAAGAA TCGGCCTCCA GAGCCAGGAA
    GAAGCTGAGA AAAAGATGAC CAAGATTCCA CCTCCGGTAC CAAAAAAGCC CAGTGTGCTC
    TACCTGCCTC TCACCTCCCC TGTAGCTCAA ATGGATGCCT GCATGGCAGA ACCAAGGCTA
    CCTTTCAGCC CCATCATCAC CCTGGAGGAA GATGGCAAGT GTCCCTCCAC TGGTGATGAC
    CAGAAGTCAC CTGGTAAAGG GGTGACTTCG CCTCTTCACA CTGACACAGA AAAAGAGGCC
    ATCTCTCCAG GGAGGTCAGT GGAACCGAGT GCTGAAGAGA AAAGTTTAAT CAGTGATAAA
    ACAGCCGAAT GGATTGCAGA GGAGGAGGAT GACGTGTTTG TGGCTTCCCG CACAACTGAA
    GATTTATTTA CTGTGATACA CAGGTCCAAA AGAAAGCTAC TGGGCTGGAA AGAGACTGGG
    GAGGGCTTCA CGGGAAGCAA ACCCAGCTCC CATTCACCAG TGAAGAATAC AGCTGATTCT
    CCCACCGGTG AGGCTGCTGC TGCCCCAGGG CCAAGTAGCA GTGCCTGCCT AGATGCTGGT
    AGAAATGATG ATTTCAAGGC CTTGCTCCAG AAGAAGGGAA GTAAGGCAAC TCCCAGGACC
    CGCCCCTCAG CGGCCGAGCT GCTGAAGACC ACTAACCCTC TGGCTCGAAG GATTATTGCA
    CAGTTCTCAA AAGACTACGA ACCCACTGAC AACCCCAGTA CCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu56088
    Clone ID Related Accession (Same CDS sequence) XM_006527729.3 , XM_030251165.1 , XM_006527729.4
    Accession Version XM_006527729.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2565bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product NHS-like protein 2 isoform X17
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000086.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006527729.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    ATGGAGAGCA TGGCAGTGGT GTACAGTGTC CCCAGTTCTT GCAATGGACC AACAGAGTCA 
    ACATTCTCCA CTTCCTGGAA GGGAGATGCT TTCACATACA TGACCCCAAG TGCCAGCAGC
    CAGGGCAATC AAGTCAGTGA AAATGGAAAA AACCCTTCCT CGGGCAATTC TTGGGTCCCT
    CTGAACACAC TCCCACCTCT GGTTCCTAAG GAGGCGGCCA CGCTCTTTGT CACCCGTGAT
    AACCCAGCAG GATGCACTGG GCTACCCAGC TACTCTGAGC ACCCCACTCT ACGAAGACAG
    ATACCAGAAA GACCTCCCAA GATTGGCCTT CTTGCCCGTG GTACCTCAAG GCTGGAAACA
    GGCCCAGGTG GGACCAACAG GTTCCGGGAG CGGTCACTGT CTGTACCCAC AGACTCGGGT
    GTTACCTCAG TGGACTATGA CGAAGAACAG AAGACCAGTG AGACCCGCAT CCTGCCTTAT
    GCCAGTACAA GCTCCGAGGG CAGTAACAGT ACTGACAACA TTGCAGCCCT CAGCACTGAG
    CAGGAGGCAC GGCACAGAAG GCAAAGATCC AAAAGTATTT CACTCAAGAA GGCCAAAAAG
    AAGCCCTCTC CACCCATGCG AAGTGTCTCA CTCGTCAAAG ATGAGCCAGC CCTCCCACCA
    GAAGGCGAAT TGGTACTGCC CAAGGACCAG AGGCCTAGGA GCCTTTGCCT CTCCTTGGAA
    CACCAAGGGC ACCACCCACC TCACCCAGAT GCTCAAGGTC ACCCAGCTGT GCCAATGCTC
    AAAGATCCAG GAAGTACGCA GTTCTCTCAC CACTGGTATC TTACTGACTG GAAGTCTGGT
    GACACCTACC AGTCCTTGTC CAGCTCCAGC ACTGCCACCG GCACCACGGT CATTGAGTGC
    ACTCAAGTTC AGGGAAGCTC AGAGTCTCTG GCCTCCCCTT CCACCTCCAG AGCGACAACA
    CCCTCCCAGC TCTCCATTGA GGTGGAAGCC AGGGAAGTAG CCTCTCCTGG GAGGCCCACT
    GGACTGATGT CCCCTTCCAG TGGTTACTCC AGCCAGTCAG AGACACCCAC ACCCACTGTC
    TCCATGTCCT TGACCCTGGG CCACTTACCT CCTCCAAGTG CTAGTGTCAG AGTACGTCCA
    GTGGTACCAG AAAGGAAGTC ATCACTGCCC CCAACATCAC CAATGGAGAA AATCTGCAAG
    TCACGGTTAT CATTTGACCT ACCACTGACC TCTTCAACCA CCCTGGATCT GTCGGGGATG
    AGTATCTCCA TCCGCAGCAA AACCAAGGTG AGCCGCCATC ACTCTGATAC CAATTTTGGG
    GTCAAACTGG CACAGAAAAC TAGTCCAAAC CAACCGATCA TGCCCATGGT TACTCAGTCT
    GACCTCCGTT CTGTTCGCCT GAGGTCAGTC AGCAAGTCTG AGCCAGAAGA CGACATTGAG
    AGTCCAGATT ACATAGAGGA ACCTGGAGCA GAGGAAGTCT TCACCATGCC AGAGAGAAAA
    GTGAAACCTC CCATAGCCGA GAAACCTCCA TTGGCCCGAA GGCCTCCAAG TTTGGTCCAC
    AGGCCGCCCT CTCTCCCTGG GGAGTATCCA CTAACTTCTC CTACTATGGC TATGGCATCC
    AGGAGCTCCA TTCCACACAT GAAGCAGCTT CCCCAAGACA GCTACACAGT GTTGCGGAAA
    CCAAAGTCAC CCAGCTTCCC CGGGGAGTCA ACAGCATCCT CCAGCCTTGT CCTCTCACCT
    CTTGCCAGTT CCTCTGGTGC TTTCTTCTCA GGAACACAGC AACCTCCCCA GGCCAGTGTA
    GAAGACGGGG GCCCCAAGGT GAGAGCCCTG CCTGAAAGAA TCGGCCTCCA GAGCCAGGAA
    GAAGCTGAGA AAAAGATGAC CAAGATTCCA CCTCCGGTAC CAAAAAAGCC CAGTGTGCTC
    TACCTGCCTC TCACCTCCCC TGTAGCTCAA ATGGATGCCT GCATGGCAGA ACCAAGGCTA
    CCTTTCAGCC CCATCATCAC CCTGGAGGAA GATGGCAAGT GTCCCTCCAC TGGTGATGAC
    CAGAAGTCAC CTGGTAAAGG GGTGACTTCG CCTCTTCACA CTGACACAGA AAAAGAGGCC
    ATCTCTCCAG GGAGGTCAGT GGAACCGAGT GCTGAAGAGA AAAGTTTAAT CAGTGATAAA
    ACAGCCGAAT GGATTGCAGA GGAGGAGGAT GACGTGTTTG TGGCTTCCCG CACAACTGAA
    GATTTATTTA CTGTGATACA CAGGTCCAAA AGAAAGCTAC TGGGCTGGAA AGAGACTGGG
    GAGGGCTTCA CGGGAAGCAA ACCCAGCTCC CATTCACCAG TGAAGAATAC AGCTGATTCT
    CCCACCGGTG AGGCTGCTGC TGCCCCAGGG CCAAGTAGCA GTGCCTGCCT AGATGCTGGT
    AGAAATGATG ATTTCAAGGC CTTGCTCCAG AAGAAGGGAA GTAAGGCAAC TCCCAGGACC
    CGCCCCTCAG CGGCCGAGCT GCTGAAGACC ACTAACCCTC TGGCTCGAAG GATTATTGCA
    CAGTTCTCAA AAGACTACGA ACCCACTGAC AACCCCAGTA CCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006527792.1
    CDS2006..4570
    Translation

    Target ORF information:

    RefSeq Version XM_006527729.4
    Organism Mus musculus(house mouse)
    Definition Mus musculus NHS-like 2 (Nhsl2), transcript variant X18, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006527729.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    ATGGAGAGCA TGGCAGTGGT GTACAGTGTC CCCAGTTCTT GCAATGGACC AACAGAGTCA 
    ACATTCTCCA CTTCCTGGAA GGGAGATGCT TTCACATACA TGACCCCAAG TGCCAGCAGC
    CAGGGCAATC AAGTCAGTGA AAATGGAAAA AACCCTTCCT CGGGCAATTC TTGGGTCCCT
    CTGAACACAC TCCCACCTCT GGTTCCTAAG GAGGCGGCCA CGCTCTTTGT CACCCGTGAT
    AACCCAGCAG GATGCACTGG GCTACCCAGC TACTCTGAGC ACCCCACTCT ACGAAGACAG
    ATACCAGAAA GACCTCCCAA GATTGGCCTT CTTGCCCGTG GTACCTCAAG GCTGGAAACA
    GGCCCAGGTG GGACCAACAG GTTCCGGGAG CGGTCACTGT CTGTACCCAC AGACTCGGGT
    GTTACCTCAG TGGACTATGA CGAAGAACAG AAGACCAGTG AGACCCGCAT CCTGCCTTAT
    GCCAGTACAA GCTCCGAGGG CAGTAACAGT ACTGACAACA TTGCAGCCCT CAGCACTGAG
    CAGGAGGCAC GGCACAGAAG GCAAAGATCC AAAAGTATTT CACTCAAGAA GGCCAAAAAG
    AAGCCCTCTC CACCCATGCG AAGTGTCTCA CTCGTCAAAG ATGAGCCAGC CCTCCCACCA
    GAAGGCGAAT TGGTACTGCC CAAGGACCAG AGGCCTAGGA GCCTTTGCCT CTCCTTGGAA
    CACCAAGGGC ACCACCCACC TCACCCAGAT GCTCAAGGTC ACCCAGCTGT GCCAATGCTC
    AAAGATCCAG GAAGTACGCA GTTCTCTCAC CACTGGTATC TTACTGACTG GAAGTCTGGT
    GACACCTACC AGTCCTTGTC CAGCTCCAGC ACTGCCACCG GCACCACGGT CATTGAGTGC
    ACTCAAGTTC AGGGAAGCTC AGAGTCTCTG GCCTCCCCTT CCACCTCCAG AGCGACAACA
    CCCTCCCAGC TCTCCATTGA GGTGGAAGCC AGGGAAGTAG CCTCTCCTGG GAGGCCCACT
    GGACTGATGT CCCCTTCCAG TGGTTACTCC AGCCAGTCAG AGACACCCAC ACCCACTGTC
    TCCATGTCCT TGACCCTGGG CCACTTACCT CCTCCAAGTG CTAGTGTCAG AGTACGTCCA
    GTGGTACCAG AAAGGAAGTC ATCACTGCCC CCAACATCAC CAATGGAGAA AATCTGCAAG
    TCACGGTTAT CATTTGACCT ACCACTGACC TCTTCAACCA CCCTGGATCT GTCGGGGATG
    AGTATCTCCA TCCGCAGCAA AACCAAGGTG AGCCGCCATC ACTCTGATAC CAATTTTGGG
    GTCAAACTGG CACAGAAAAC TAGTCCAAAC CAACCGATCA TGCCCATGGT TACTCAGTCT
    GACCTCCGTT CTGTTCGCCT GAGGTCAGTC AGCAAGTCTG AGCCAGAAGA CGACATTGAG
    AGTCCAGATT ACATAGAGGA ACCTGGAGCA GAGGAAGTCT TCACCATGCC AGAGAGAAAA
    GTGAAACCTC CCATAGCCGA GAAACCTCCA TTGGCCCGAA GGCCTCCAAG TTTGGTCCAC
    AGGCCGCCCT CTCTCCCTGG GGAGTATCCA CTAACTTCTC CTACTATGGC TATGGCATCC
    AGGAGCTCCA TTCCACACAT GAAGCAGCTT CCCCAAGACA GCTACACAGT GTTGCGGAAA
    CCAAAGTCAC CCAGCTTCCC CGGGGAGTCA ACAGCATCCT CCAGCCTTGT CCTCTCACCT
    CTTGCCAGTT CCTCTGGTGC TTTCTTCTCA GGAACACAGC AACCTCCCCA GGCCAGTGTA
    GAAGACGGGG GCCCCAAGGT GAGAGCCCTG CCTGAAAGAA TCGGCCTCCA GAGCCAGGAA
    GAAGCTGAGA AAAAGATGAC CAAGATTCCA CCTCCGGTAC CAAAAAAGCC CAGTGTGCTC
    TACCTGCCTC TCACCTCCCC TGTAGCTCAA ATGGATGCCT GCATGGCAGA ACCAAGGCTA
    CCTTTCAGCC CCATCATCAC CCTGGAGGAA GATGGCAAGT GTCCCTCCAC TGGTGATGAC
    CAGAAGTCAC CTGGTAAAGG GGTGACTTCG CCTCTTCACA CTGACACAGA AAAAGAGGCC
    ATCTCTCCAG GGAGGTCAGT GGAACCGAGT GCTGAAGAGA AAAGTTTAAT CAGTGATAAA
    ACAGCCGAAT GGATTGCAGA GGAGGAGGAT GACGTGTTTG TGGCTTCCCG CACAACTGAA
    GATTTATTTA CTGTGATACA CAGGTCCAAA AGAAAGCTAC TGGGCTGGAA AGAGACTGGG
    GAGGGCTTCA CGGGAAGCAA ACCCAGCTCC CATTCACCAG TGAAGAATAC AGCTGATTCT
    CCCACCGGTG AGGCTGCTGC TGCCCCAGGG CCAAGTAGCA GTGCCTGCCT AGATGCTGGT
    AGAAATGATG ATTTCAAGGC CTTGCTCCAG AAGAAGGGAA GTAAGGCAAC TCCCAGGACC
    CGCCCCTCAG CGGCCGAGCT GCTGAAGACC ACTAACCCTC TGGCTCGAAG GATTATTGCA
    CAGTTCTCAA AAGACTACGA ACCCACTGAC AACCCCAGTA CCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu56076
    Clone ID Related Accession (Same CDS sequence) XM_030251161.1
    Accession Version XM_030251161.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3765bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product NHS-like protein 2 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000086.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGAGGAGGT GGGCGTGGGA GGAGACCAAC GTGCGGCGAC AGGGCGACCC GGCGCAAGCG 
    AGCCCGGGCC ACACCCACAG GGGCTCGGTC CGGGACGCGT TACCTCGTGG CAGGGGTCGG
    AGAGAGAGCA GGCAGCAGCT CGGGACCTGG AGGCGGCAGG CGCAGGAGAC AGACGAGACG
    CTGGCAGAGG TGACGCCAAC AGAGGCAGGC AGAGTAGGAG GGAAGGGACT GCGGCAGGAT
    CTGTCGAGGA AAAATCTTGC AGACAGCGAT CCCTCTCGCC TTTTAAGCTC AGCTACGCGC
    GCCCCCCGCA GGGGCGGGCC TTCGGGAACC GAGGGAGCCC ACTGGCTCCC GCGGCTGCGC
    TCTCACTCAC TCTCTCCTTC CCTAGCTGCA GCTAACTCGG GTCGGGAAAA TGCGACAGCG
    ACTGCCCACT CGAGGTCGTC ATGGCGACAG CCAGTGAACG TGTTCCTCTC CTCGGGCAGG
    CCCCCGAGTG TAGAGGAACT GCTTCGCGAG GCGCAGCTCA ATCTCCAGAG CCTGTTGCAA
    GAAGAATATG AGGAACAGTA TTCGGAGGCC AGACTTCTGG GGCAGACCTT CCGCTCTTCT
    GATGGGGCCC CTGAGCCTAC TCCCAGCCCA AGGCCCCAGT CTGCCAAGCG TCTGGAGTTT
    GTACTGATGC CTGCAAAACG GCAGCTGAGC GAGGATGAGA CCACGACCCA GGGTGTGAGG
    GCCCCTGAGG CCTGCCTGAG CCTGTCTACT GCCAACAAGC AAAGTGCCTG GAATGACCCC
    TTCCCTCTAC CCATCCTAAA GGAGAGGCTC TGGCTTCAGC CCTGCTCCAC ACAGTCTGAC
    CTTGTGCCAA TCAACATCTC TGGGCAGCAG TTTGATAGAC ATGCAAGTTT TCGACACTCG
    TTGTTTAACA CAGAGACAGC CGTGAACCCC AAGTCCACCC TGAGGCGGAG GCGGACCATT
    ATTGGATTCT CTAACTTTTC TCAGCGAGAC CAAGGTCACA GCAGCAGCCC CACAGGCAGC
    CTGGCCCGCT CTGCCACCTC AGACATCAGG CCTGGCCACT CAGCTCCACA AGTTGTTCAG
    GGAAGAGTTG CTGTTGGGCA GGAAGCTCGG TTCCCAAGTC TCACCTCACC AGGACTGAGA
    CACTCTTCTA GTGAGCCCGG AGACGCTCAC CAGGCACGCA GTGCCTCAGA CCATCCCGGC
    ATGGAGAGCA TGGCAGTGGT GTACAGTGTC CCCAGTTCTT GCAATGGACC AACAGAGTCA
    ACATTCTCCA CTTCCTGGAA GGGAGATGCT TTCACATACA TGACCCCAAG TGCCAGCAGC
    CAGGGCAATC AAGTCAGTGA AAATGGAAAA AACCCTTCCT CGGGCAATTC TTGGGTCCCT
    CTGAACACAC TCCCACCTCT GGTTCCTAAG GAGGCGGCCA CGCTCTTTGT CACCCGTGAT
    AACCCAGCAG GATGCACTGG GCTACCCAGC TACTCTGAGC ACCCCACTCT ACGAAGACAG
    ATACCAGAAA GACCTCCCAA GATTGGCCTT CTTGCCCGTG GTACCTCAAG GCTGGAAACA
    GGCCCAGGTG GGACCAACAG GTTCCGGGAG CGGTCACTGT CTGTACCCAC AGACTCGGGT
    GTTACCTCAG TGGACTATGA CGAAGAACAG AAGACCAGTG AGACCCGCAT CCTGCCTTAT
    GCCAGTACAA GCTCCGAGGG CAGTAACAGT ACTGACAACA TTGCAGCCCT CAGCACTGAG
    CAGGAGGCAC GGCACAGAAG GCAAAGATCC AAAAGTATTT CACTCAAGAA GGCCAAAAAG
    AAGCCCTCTC CACCCATGCG AAGTGTCTCA CTCGTCAAAG ATGAGCCAGC CCTCCCACCA
    GAAGGCGAAT TGGTACTGCC CAAGGACCAG AGGCCTAGGA GCCTTTGCCT CTCCTTGGAA
    CACCAAGGGC ACCACCCACC TCACCCAGAT GCTCAAGGTC ACCCAGCTGT GCCAATGCTC
    AAAGATCCAG GAAGTACGCA GTTCTCTCAC CACTGGTATC TTACTGACTG GAAGTCTGGT
    GACACCTACC AGTCCTTGTC CAGCTCCAGC ACTGCCACCG GCACCACGGT CATTGAGTGC
    ACTCAAGTTC AGGGAAGCTC AGAGTCTCTG GCCTCCCCTT CCACCTCCAG AGCGACAACA
    CCCTCCCAGC TCTCCATTGA GGTGGAAGCC AGGGAAGTAG CCTCTCCTGG GAGGCCCACT
    GGACTGATGT CCCCTTCCAG TGGTTACTCC AGCCAGTCAG AGACACCCAC ACCCACTGTC
    TCCATGTCCT TGACCCTGGG CCACTTACCT CCTCCAAGTG CTAGTGTCAG AGTACGTCCA
    GTGGTACCAG AAAGGAAGTC ATCACTGCCC CCAACATCAC CAATGGAGAA AATCTGCAAG
    TCACGGTTAT CATTTGACCT ACCACTGACC TCTTCAACCA CCCTGGATCT GTCGGGGATG
    AGTATCTCCA TCCGCAGCAA AACCAAGGTG AGCCGCCATC ACTCTGATAC CAATTTTGGG
    GTCAAACTGG CACAGAAAAC TAGTCCAAAC CAACCGATCA TGCCCATGGT TACTCAGTCT
    GACCTCCGTT CTGTTCGCCT GAGGTCAGTC AGCAAGTCTG AGCCAGAAGA CGACATTGAG
    AGTCCAGATT ACATAGAGGA ACCTGGAGCA GAGGAAGTCT TCACCATGCC AGAGAGAAAA
    GTGAAACCTC CCATAGCCGA GAAACCTCCA TTGGCCCGAA GGCCTCCAAG TTTGGTCCAC
    AGGCCGCCCT CTCTCCCTGG GGAGTATCCA CTAACTTCTC CTACTATGGC TATGGCATCC
    AGGAGCTCCA TTCCACACAT GAAGCAGCTT CCCCAAGACA GCTACACAGT GTTGCGGAAA
    CCAAAGTCAC CCAGCTTCCC CGGGGAGTCA ACAGCATCCT CCAGCCTTGT CCTCTCACCT
    CTTGCCAGTT CCTCTGGTGC TTTCTTCTCA GGAACACAGC AACCTCCCCA GGCCAGTGTA
    GAAGACGGGG GCCCCAAGGT GAGAGCCCTG CCTGAAAGAA TCGGCCTCCA GAGCCAGGAA
    GAAGCTGAGA AAAAGATGAC CAAGATTCCA CCTCCGGTAC CAAAAAAGCC CAGTGTGCTC
    TACCTGCCTC TCACCTCCCC TGTAGCTCAA ATGGATGCCT GCATGGCAGA ACCAAGGCTA
    CCTTTCAGCC CCATCATCAC CCTGGAGGAA GATGGCAAGT GTCCCTCCAC TGGTGATGAC
    CAGAAGTCAC CTGGTAAAGG GGTGACTTCG CCTCTTCACA CTGACACAGA AAAAGAGGCC
    ATCTCTCCAG GGAGGTCAGT GGAACCGAGT GCTGAAGAGA AAAGTTTAAT CAGTGATAAA
    ACAGCCGAAT GGATTGCAGA GGAGGAGGAT GACGTGTTTG TGGCTTCCCG CACAACTGAA
    GATTTATTTA CTGTGATACA CAGGTCCAAA AGAAAGCTAC TGGGCTGGAA AGAGACTGGG
    GAGGGCTTCA CGGGAAGCAA ACCCAGCTCC CATTCACCAG TGAAGAATAC AGCTGATTCT
    CCCACCGGTG AGGCTGCTGC TGCCCCAGGG CCAAGTAGCA GTGCCTGCCT AGATGCTGGT
    AGAAATGATG ATTTCAAGGC CTTGCTCCAG AAGAAGGGAA GTAAGGCAAC TCCCAGGACC
    CGCCCCTCAG CGGCCGAGCT GCTGAAGACC ACTAACCCTC TGGCTCGAAG GATTATTGCA
    CAGTTCTCAA AAGACTACGA ACCCACTGAC AACCCCAGTA CCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_030107021.1
    CDS20..3784
    Translation

    Target ORF information:

    RefSeq Version XM_030251161.1
    Organism Mus musculus(house mouse)
    Definition Mus musculus NHS-like 2 (Nhsl2), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_030251161.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGAGGAGGT GGGCGTGGGA GGAGACCAAC GTGCGGCGAC AGGGCGACCC GGCGCAAGCG 
    AGCCCGGGCC ACACCCACAG GGGCTCGGTC CGGGACGCGT TACCTCGTGG CAGGGGTCGG
    AGAGAGAGCA GGCAGCAGCT CGGGACCTGG AGGCGGCAGG CGCAGGAGAC AGACGAGACG
    CTGGCAGAGG TGACGCCAAC AGAGGCAGGC AGAGTAGGAG GGAAGGGACT GCGGCAGGAT
    CTGTCGAGGA AAAATCTTGC AGACAGCGAT CCCTCTCGCC TTTTAAGCTC AGCTACGCGC
    GCCCCCCGCA GGGGCGGGCC TTCGGGAACC GAGGGAGCCC ACTGGCTCCC GCGGCTGCGC
    TCTCACTCAC TCTCTCCTTC CCTAGCTGCA GCTAACTCGG GTCGGGAAAA TGCGACAGCG
    ACTGCCCACT CGAGGTCGTC ATGGCGACAG CCAGTGAACG TGTTCCTCTC CTCGGGCAGG
    CCCCCGAGTG TAGAGGAACT GCTTCGCGAG GCGCAGCTCA ATCTCCAGAG CCTGTTGCAA
    GAAGAATATG AGGAACAGTA TTCGGAGGCC AGACTTCTGG GGCAGACCTT CCGCTCTTCT
    GATGGGGCCC CTGAGCCTAC TCCCAGCCCA AGGCCCCAGT CTGCCAAGCG TCTGGAGTTT
    GTACTGATGC CTGCAAAACG GCAGCTGAGC GAGGATGAGA CCACGACCCA GGGTGTGAGG
    GCCCCTGAGG CCTGCCTGAG CCTGTCTACT GCCAACAAGC AAAGTGCCTG GAATGACCCC
    TTCCCTCTAC CCATCCTAAA GGAGAGGCTC TGGCTTCAGC CCTGCTCCAC ACAGTCTGAC
    CTTGTGCCAA TCAACATCTC TGGGCAGCAG TTTGATAGAC ATGCAAGTTT TCGACACTCG
    TTGTTTAACA CAGAGACAGC CGTGAACCCC AAGTCCACCC TGAGGCGGAG GCGGACCATT
    ATTGGATTCT CTAACTTTTC TCAGCGAGAC CAAGGTCACA GCAGCAGCCC CACAGGCAGC
    CTGGCCCGCT CTGCCACCTC AGACATCAGG CCTGGCCACT CAGCTCCACA AGTTGTTCAG
    GGAAGAGTTG CTGTTGGGCA GGAAGCTCGG TTCCCAAGTC TCACCTCACC AGGACTGAGA
    CACTCTTCTA GTGAGCCCGG AGACGCTCAC CAGGCACGCA GTGCCTCAGA CCATCCCGGC
    ATGGAGAGCA TGGCAGTGGT GTACAGTGTC CCCAGTTCTT GCAATGGACC AACAGAGTCA
    ACATTCTCCA CTTCCTGGAA GGGAGATGCT TTCACATACA TGACCCCAAG TGCCAGCAGC
    CAGGGCAATC AAGTCAGTGA AAATGGAAAA AACCCTTCCT CGGGCAATTC TTGGGTCCCT
    CTGAACACAC TCCCACCTCT GGTTCCTAAG GAGGCGGCCA CGCTCTTTGT CACCCGTGAT
    AACCCAGCAG GATGCACTGG GCTACCCAGC TACTCTGAGC ACCCCACTCT ACGAAGACAG
    ATACCAGAAA GACCTCCCAA GATTGGCCTT CTTGCCCGTG GTACCTCAAG GCTGGAAACA
    GGCCCAGGTG GGACCAACAG GTTCCGGGAG CGGTCACTGT CTGTACCCAC AGACTCGGGT
    GTTACCTCAG TGGACTATGA CGAAGAACAG AAGACCAGTG AGACCCGCAT CCTGCCTTAT
    GCCAGTACAA GCTCCGAGGG CAGTAACAGT ACTGACAACA TTGCAGCCCT CAGCACTGAG
    CAGGAGGCAC GGCACAGAAG GCAAAGATCC AAAAGTATTT CACTCAAGAA GGCCAAAAAG
    AAGCCCTCTC CACCCATGCG AAGTGTCTCA CTCGTCAAAG ATGAGCCAGC CCTCCCACCA
    GAAGGCGAAT TGGTACTGCC CAAGGACCAG AGGCCTAGGA GCCTTTGCCT CTCCTTGGAA
    CACCAAGGGC ACCACCCACC TCACCCAGAT GCTCAAGGTC ACCCAGCTGT GCCAATGCTC
    AAAGATCCAG GAAGTACGCA GTTCTCTCAC CACTGGTATC TTACTGACTG GAAGTCTGGT
    GACACCTACC AGTCCTTGTC CAGCTCCAGC ACTGCCACCG GCACCACGGT CATTGAGTGC
    ACTCAAGTTC AGGGAAGCTC AGAGTCTCTG GCCTCCCCTT CCACCTCCAG AGCGACAACA
    CCCTCCCAGC TCTCCATTGA GGTGGAAGCC AGGGAAGTAG CCTCTCCTGG GAGGCCCACT
    GGACTGATGT CCCCTTCCAG TGGTTACTCC AGCCAGTCAG AGACACCCAC ACCCACTGTC
    TCCATGTCCT TGACCCTGGG CCACTTACCT CCTCCAAGTG CTAGTGTCAG AGTACGTCCA
    GTGGTACCAG AAAGGAAGTC ATCACTGCCC CCAACATCAC CAATGGAGAA AATCTGCAAG
    TCACGGTTAT CATTTGACCT ACCACTGACC TCTTCAACCA CCCTGGATCT GTCGGGGATG
    AGTATCTCCA TCCGCAGCAA AACCAAGGTG AGCCGCCATC ACTCTGATAC CAATTTTGGG
    GTCAAACTGG CACAGAAAAC TAGTCCAAAC CAACCGATCA TGCCCATGGT TACTCAGTCT
    GACCTCCGTT CTGTTCGCCT GAGGTCAGTC AGCAAGTCTG AGCCAGAAGA CGACATTGAG
    AGTCCAGATT ACATAGAGGA ACCTGGAGCA GAGGAAGTCT TCACCATGCC AGAGAGAAAA
    GTGAAACCTC CCATAGCCGA GAAACCTCCA TTGGCCCGAA GGCCTCCAAG TTTGGTCCAC
    AGGCCGCCCT CTCTCCCTGG GGAGTATCCA CTAACTTCTC CTACTATGGC TATGGCATCC
    AGGAGCTCCA TTCCACACAT GAAGCAGCTT CCCCAAGACA GCTACACAGT GTTGCGGAAA
    CCAAAGTCAC CCAGCTTCCC CGGGGAGTCA ACAGCATCCT CCAGCCTTGT CCTCTCACCT
    CTTGCCAGTT CCTCTGGTGC TTTCTTCTCA GGAACACAGC AACCTCCCCA GGCCAGTGTA
    GAAGACGGGG GCCCCAAGGT GAGAGCCCTG CCTGAAAGAA TCGGCCTCCA GAGCCAGGAA
    GAAGCTGAGA AAAAGATGAC CAAGATTCCA CCTCCGGTAC CAAAAAAGCC CAGTGTGCTC
    TACCTGCCTC TCACCTCCCC TGTAGCTCAA ATGGATGCCT GCATGGCAGA ACCAAGGCTA
    CCTTTCAGCC CCATCATCAC CCTGGAGGAA GATGGCAAGT GTCCCTCCAC TGGTGATGAC
    CAGAAGTCAC CTGGTAAAGG GGTGACTTCG CCTCTTCACA CTGACACAGA AAAAGAGGCC
    ATCTCTCCAG GGAGGTCAGT GGAACCGAGT GCTGAAGAGA AAAGTTTAAT CAGTGATAAA
    ACAGCCGAAT GGATTGCAGA GGAGGAGGAT GACGTGTTTG TGGCTTCCCG CACAACTGAA
    GATTTATTTA CTGTGATACA CAGGTCCAAA AGAAAGCTAC TGGGCTGGAA AGAGACTGGG
    GAGGGCTTCA CGGGAAGCAA ACCCAGCTCC CATTCACCAG TGAAGAATAC AGCTGATTCT
    CCCACCGGTG AGGCTGCTGC TGCCCCAGGG CCAAGTAGCA GTGCCTGCCT AGATGCTGGT
    AGAAATGATG ATTTCAAGGC CTTGCTCCAG AAGAAGGGAA GTAAGGCAAC TCCCAGGACC
    CGCCCCTCAG CGGCCGAGCT GCTGAAGACC ACTAACCCTC TGGCTCGAAG GATTATTGCA
    CAGTTCTCAA AAGACTACGA ACCCACTGAC AACCCCAGTA CCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu56077
    Clone ID Related Accession (Same CDS sequence) XM_006527718.4 , XM_006527718.3
    Accession Version XM_006527718.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3450bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product NHS-like protein 2 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000086.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006527718.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGATGGGCA ACTCTCACCA CAAGCAACCG AGGAGTAAGA GCCAAAGCAG CAGGATGCAT 
    TCAGCAACAG CTGCAGCTAA CTCGGGTCGG GAAAATGCGA CAGCGACTGC CCACTCGAGG
    TCGTCATGGC GACAGCCAGT GAACGTGTTC CTCTCCTCGG GCAGGCCCCC GAGTGTAGAG
    GAACTGCTTC GCGAGGCGCA GCTCAATCTC CAGAGCCTGT TGCAAGAAGA ATATGAGGAA
    CAGTATTCGG AGGCCAGACT TCTGGGGCAG ACCTTCCGCT CTTCTGATGG GGCCCCTGAG
    CCTACTCCCA GCCCAAGGCC CCAGTCTGCC AAGCGTCTGG AGTTTGTACT GATGCCTGCA
    AAACGGCAGC TGAGCGAGGA TGAGACCACG ACCCAGGGTG TGAGGGCCCC TGAGGCCTGC
    CTGAGCCTGT CTACTGCCAA CAAGCAAAGT GCCTGGAATG ACCCCTTCCC TCTACCCATC
    CTAAAGGAGA GGCTCTGGCT TCAGCCCTGC TCCACACAGT CTGACCTTGT GCCAATCAAC
    ATCTCTGGGC AGCAGTTTGA TAGACATGCA AGTTTTCGAC ACTCGTTGTT TAACACAGAG
    ACAGCCGTGA ACCCCAAGTC CACCCTGAGG CGGAGGCGGA CCATTATTGG ATTCTCTAAC
    TTTTCTCAGC GAGACCAAGG TCACAGCAGC AGCCCCACAG GCAGCCTGGC CCGCTCTGCC
    ACCTCAGACA TCAGGCCTGG CCACTCAGCT CCACAAGTTG TTCAGGGAAG AGTTGCTGTT
    GGGCAGGAAG CTCGGTTCCC AAGTCTCACC TCACCAGGAC TGAGACACTC TTCTAGTGAG
    CCCGGAGACG CTCACCAGGC ACGCAGTGCC TCAGACCATC CCGGCATGGA GAGCATGGCA
    GTGGTGTACA GTGTCCCCAG TTCTTGCAAT GGACCAACAG AGTCAACATT CTCCACTTCC
    TGGAAGGGAG ATGCTTTCAC ATACATGACC CCAAGTGCCA GCAGCCAGGG CAATCAAGTC
    AGTGAAAATG GAAAAAACCC TTCCTCGGGC AATTCTTGGG TCCCTCTGAA CACACTCCCA
    CCTCTGGTTC CTAAGGAGGC GGCCACGCTC TTTGTCACCC GTGATAACCC AGCAGGATGC
    ACTGGGCTAC CCAGCTACTC TGAGCACCCC ACTCTACGAA GACAGATACC AGAAAGACCT
    CCCAAGATTG GCCTTCTTGC CCGTGGTACC TCAAGGCTGG AAACAGGCCC AGGTGGGACC
    AACAGGTTCC GGGAGCGGTC ACTGTCTGTA CCCACAGACT CGGGTGTTAC CTCAGTGGAC
    TATGACGAAG AACAGAAGAC CAGTGAGACC CGCATCCTGC CTTATGCCAG TACAAGCTCC
    GAGGGCAGTA ACAGTACTGA CAACATTGCA GCCCTCAGCA CTGAGCAGGA GGCACGGCAC
    AGAAGGCAAA GATCCAAAAG TATTTCACTC AAGAAGGCCA AAAAGAAGCC CTCTCCACCC
    ATGCGAAGTG TCTCACTCGT CAAAGATGAG CCAGCCCTCC CACCAGAAGG CGAATTGGTA
    CTGCCCAAGG ACCAGAGGCC TAGGAGCCTT TGCCTCTCCT TGGAACACCA AGGGCACCAC
    CCACCTCACC CAGATGCTCA AGGTCACCCA GCTGTGCCAA TGCTCAAAGA TCCAGGAAGT
    ACGCAGTTCT CTCACCACTG GTATCTTACT GACTGGAAGT CTGGTGACAC CTACCAGTCC
    TTGTCCAGCT CCAGCACTGC CACCGGCACC ACGGTCATTG AGTGCACTCA AGTTCAGGGA
    AGCTCAGAGT CTCTGGCCTC CCCTTCCACC TCCAGAGCGA CAACACCCTC CCAGCTCTCC
    ATTGAGGTGG AAGCCAGGGA AGTAGCCTCT CCTGGGAGGC CCACTGGACT GATGTCCCCT
    TCCAGTGGTT ACTCCAGCCA GTCAGAGACA CCCACACCCA CTGTCTCCAT GTCCTTGACC
    CTGGGCCACT TACCTCCTCC AAGTGCTAGT GTCAGAGTAC GTCCAGTGGT ACCAGAAAGG
    AAGTCATCAC TGCCCCCAAC ATCACCAATG GAGAAAATCT GCAAGTCACG GTTATCATTT
    GACCTACCAC TGACCTCTTC AACCACCCTG GATCTGTCGG GGATGAGTAT CTCCATCCGC
    AGCAAAACCA AGGTGAGCCG CCATCACTCT GATACCAATT TTGGGGTCAA ACTGGCACAG
    AAAACTAGTC CAAACCAACC GATCATGCCC ATGGTTACTC AGTCTGACCT CCGTTCTGTT
    CGCCTGAGGT CAGTCAGCAA GTCTGAGCCA GAAGACGACA TTGAGAGTCC AGATTACATA
    GAGGAACCTG GAGCAGAGGA AGTCTTCACC ATGCCAGAGA GAAAAGTGAA ACCTCCCATA
    GCCGAGAAAC CTCCATTGGC CCGAAGGCCT CCAAGTTTGG TCCACAGGCC GCCCTCTCTC
    CCTGGGGAGT ATCCACTAAC TTCTCCTACT ATGGCTATGG CATCCAGGAG CTCCATTCCA
    CACATGAAGC AGCTTCCCCA AGACAGCTAC ACAGTGTTGC GGAAACCAAA GTCACCCAGC
    TTCCCCGGGG AGTCAACAGC ATCCTCCAGC CTTGTCCTCT CACCTCTTGC CAGTTCCTCT
    GGTGCTTTCT TCTCAGGAAC ACAGCAACCT CCCCAGGCCA GTGTAGAAGA CGGGGGCCCC
    AAGGTGAGAG CCCTGCCTGA AAGAATCGGC CTCCAGAGCC AGGAAGAAGC TGAGAAAAAG
    ATGACCAAGA TTCCACCTCC GGTACCAAAA AAGCCCAGTG TGCTCTACCT GCCTCTCACC
    TCCCCTGTAG CTCAAATGGA TGCCTGCATG GCAGAACCAA GGCTACCTTT CAGCCCCATC
    ATCACCCTGG AGGAAGATGG CAAGTGTCCC TCCACTGGTG ATGACCAGAA GTCACCTGGT
    AAAGGGGTGA CTTCGCCTCT TCACACTGAC ACAGAAAAAG AGGCCATCTC TCCAGGGAGG
    TCAGTGGAAC CGAGTGCTGA AGAGAAAAGT TTAATCAGTG ATAAAACAGC CGAATGGATT
    GCAGAGGAGG AGGATGACGT GTTTGTGGCT TCCCGCACAA CTGAAGATTT ATTTACTGTG
    ATACACAGGT CCAAAAGAAA GCTACTGGGC TGGAAAGAGA CTGGGGAGGG CTTCACGGGA
    AGCAAACCCA GCTCCCATTC ACCAGTGAAG AATACAGCTG ATTCTCCCAC CGGTGAGGCT
    GCTGCTGCCC CAGGGCCAAG TAGCAGTGCC TGCCTAGATG CTGGTAGAAA TGATGATTTC
    AAGGCCTTGC TCCAGAAGAA GGGAAGTAAG GCAACTCCCA GGACCCGCCC CTCAGCGGCC
    GAGCTGCTGA AGACCACTAA CCCTCTGGCT CGAAGGATTA TTGCACAGTT CTCAAAAGAC
    TACGAACCCA CTGACAACCC CAGTACCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006527781.1
    CDS9856..13305
    Translation

    Target ORF information:

    RefSeq Version XM_006527718.4
    Organism Mus musculus(house mouse)
    Definition Mus musculus NHS-like 2 (Nhsl2), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006527718.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGATGGGCA ACTCTCACCA CAAGCAACCG AGGAGTAAGA GCCAAAGCAG CAGGATGCAT 
    TCAGCAACAG CTGCAGCTAA CTCGGGTCGG GAAAATGCGA CAGCGACTGC CCACTCGAGG
    TCGTCATGGC GACAGCCAGT GAACGTGTTC CTCTCCTCGG GCAGGCCCCC GAGTGTAGAG
    GAACTGCTTC GCGAGGCGCA GCTCAATCTC CAGAGCCTGT TGCAAGAAGA ATATGAGGAA
    CAGTATTCGG AGGCCAGACT TCTGGGGCAG ACCTTCCGCT CTTCTGATGG GGCCCCTGAG
    CCTACTCCCA GCCCAAGGCC CCAGTCTGCC AAGCGTCTGG AGTTTGTACT GATGCCTGCA
    AAACGGCAGC TGAGCGAGGA TGAGACCACG ACCCAGGGTG TGAGGGCCCC TGAGGCCTGC
    CTGAGCCTGT CTACTGCCAA CAAGCAAAGT GCCTGGAATG ACCCCTTCCC TCTACCCATC
    CTAAAGGAGA GGCTCTGGCT TCAGCCCTGC TCCACACAGT CTGACCTTGT GCCAATCAAC
    ATCTCTGGGC AGCAGTTTGA TAGACATGCA AGTTTTCGAC ACTCGTTGTT TAACACAGAG
    ACAGCCGTGA ACCCCAAGTC CACCCTGAGG CGGAGGCGGA CCATTATTGG ATTCTCTAAC
    TTTTCTCAGC GAGACCAAGG TCACAGCAGC AGCCCCACAG GCAGCCTGGC CCGCTCTGCC
    ACCTCAGACA TCAGGCCTGG CCACTCAGCT CCACAAGTTG TTCAGGGAAG AGTTGCTGTT
    GGGCAGGAAG CTCGGTTCCC AAGTCTCACC TCACCAGGAC TGAGACACTC TTCTAGTGAG
    CCCGGAGACG CTCACCAGGC ACGCAGTGCC TCAGACCATC CCGGCATGGA GAGCATGGCA
    GTGGTGTACA GTGTCCCCAG TTCTTGCAAT GGACCAACAG AGTCAACATT CTCCACTTCC
    TGGAAGGGAG ATGCTTTCAC ATACATGACC CCAAGTGCCA GCAGCCAGGG CAATCAAGTC
    AGTGAAAATG GAAAAAACCC TTCCTCGGGC AATTCTTGGG TCCCTCTGAA CACACTCCCA
    CCTCTGGTTC CTAAGGAGGC GGCCACGCTC TTTGTCACCC GTGATAACCC AGCAGGATGC
    ACTGGGCTAC CCAGCTACTC TGAGCACCCC ACTCTACGAA GACAGATACC AGAAAGACCT
    CCCAAGATTG GCCTTCTTGC CCGTGGTACC TCAAGGCTGG AAACAGGCCC AGGTGGGACC
    AACAGGTTCC GGGAGCGGTC ACTGTCTGTA CCCACAGACT CGGGTGTTAC CTCAGTGGAC
    TATGACGAAG AACAGAAGAC CAGTGAGACC CGCATCCTGC CTTATGCCAG TACAAGCTCC
    GAGGGCAGTA ACAGTACTGA CAACATTGCA GCCCTCAGCA CTGAGCAGGA GGCACGGCAC
    AGAAGGCAAA GATCCAAAAG TATTTCACTC AAGAAGGCCA AAAAGAAGCC CTCTCCACCC
    ATGCGAAGTG TCTCACTCGT CAAAGATGAG CCAGCCCTCC CACCAGAAGG CGAATTGGTA
    CTGCCCAAGG ACCAGAGGCC TAGGAGCCTT TGCCTCTCCT TGGAACACCA AGGGCACCAC
    CCACCTCACC CAGATGCTCA AGGTCACCCA GCTGTGCCAA TGCTCAAAGA TCCAGGAAGT
    ACGCAGTTCT CTCACCACTG GTATCTTACT GACTGGAAGT CTGGTGACAC CTACCAGTCC
    TTGTCCAGCT CCAGCACTGC CACCGGCACC ACGGTCATTG AGTGCACTCA AGTTCAGGGA
    AGCTCAGAGT CTCTGGCCTC CCCTTCCACC TCCAGAGCGA CAACACCCTC CCAGCTCTCC
    ATTGAGGTGG AAGCCAGGGA AGTAGCCTCT CCTGGGAGGC CCACTGGACT GATGTCCCCT
    TCCAGTGGTT ACTCCAGCCA GTCAGAGACA CCCACACCCA CTGTCTCCAT GTCCTTGACC
    CTGGGCCACT TACCTCCTCC AAGTGCTAGT GTCAGAGTAC GTCCAGTGGT ACCAGAAAGG
    AAGTCATCAC TGCCCCCAAC ATCACCAATG GAGAAAATCT GCAAGTCACG GTTATCATTT
    GACCTACCAC TGACCTCTTC AACCACCCTG GATCTGTCGG GGATGAGTAT CTCCATCCGC
    AGCAAAACCA AGGTGAGCCG CCATCACTCT GATACCAATT TTGGGGTCAA ACTGGCACAG
    AAAACTAGTC CAAACCAACC GATCATGCCC ATGGTTACTC AGTCTGACCT CCGTTCTGTT
    CGCCTGAGGT CAGTCAGCAA GTCTGAGCCA GAAGACGACA TTGAGAGTCC AGATTACATA
    GAGGAACCTG GAGCAGAGGA AGTCTTCACC ATGCCAGAGA GAAAAGTGAA ACCTCCCATA
    GCCGAGAAAC CTCCATTGGC CCGAAGGCCT CCAAGTTTGG TCCACAGGCC GCCCTCTCTC
    CCTGGGGAGT ATCCACTAAC TTCTCCTACT ATGGCTATGG CATCCAGGAG CTCCATTCCA
    CACATGAAGC AGCTTCCCCA AGACAGCTAC ACAGTGTTGC GGAAACCAAA GTCACCCAGC
    TTCCCCGGGG AGTCAACAGC ATCCTCCAGC CTTGTCCTCT CACCTCTTGC CAGTTCCTCT
    GGTGCTTTCT TCTCAGGAAC ACAGCAACCT CCCCAGGCCA GTGTAGAAGA CGGGGGCCCC
    AAGGTGAGAG CCCTGCCTGA AAGAATCGGC CTCCAGAGCC AGGAAGAAGC TGAGAAAAAG
    ATGACCAAGA TTCCACCTCC GGTACCAAAA AAGCCCAGTG TGCTCTACCT GCCTCTCACC
    TCCCCTGTAG CTCAAATGGA TGCCTGCATG GCAGAACCAA GGCTACCTTT CAGCCCCATC
    ATCACCCTGG AGGAAGATGG CAAGTGTCCC TCCACTGGTG ATGACCAGAA GTCACCTGGT
    AAAGGGGTGA CTTCGCCTCT TCACACTGAC ACAGAAAAAG AGGCCATCTC TCCAGGGAGG
    TCAGTGGAAC CGAGTGCTGA AGAGAAAAGT TTAATCAGTG ATAAAACAGC CGAATGGATT
    GCAGAGGAGG AGGATGACGT GTTTGTGGCT TCCCGCACAA CTGAAGATTT ATTTACTGTG
    ATACACAGGT CCAAAAGAAA GCTACTGGGC TGGAAAGAGA CTGGGGAGGG CTTCACGGGA
    AGCAAACCCA GCTCCCATTC ACCAGTGAAG AATACAGCTG ATTCTCCCAC CGGTGAGGCT
    GCTGCTGCCC CAGGGCCAAG TAGCAGTGCC TGCCTAGATG CTGGTAGAAA TGATGATTTC
    AAGGCCTTGC TCCAGAAGAA GGGAAGTAAG GCAACTCCCA GGACCCGCCC CTCAGCGGCC
    GAGCTGCTGA AGACCACTAA CCCTCTGGCT CGAAGGATTA TTGCACAGTT CTCAAAAGAC
    TACGAACCCA CTGACAACCC CAGTACCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu56078
    Clone ID Related Accession (Same CDS sequence) XM_030251162.1
    Accession Version XM_030251162.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3444bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product NHS-like protein 2 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000086.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGAGGAGGT GGGCGTGGGA GGAGACCAAC GTGCGGCGAC AGGGCGACCC GGCGCAAGCG 
    AGCCCGGGCC ACACCCACAG GGGCTCGGTC CGGGACGCGT TACCTCGTGG CAGGGGTCGG
    AGAGAGAGCA GGCAGCAGCT CGGGACCTGG AGGCGGCAGG CGCAGGAGAC AGACGAGACG
    CTGGCAGAGG TGACGCCAAC AGAGGCAGGC AGAGTAGGAG GGAAGGGACT GCGGCAGGAT
    CTGTCGAGGA AAAATCTTGC AGACAGCGAT CCCTCTCGCC TTTTAAGCTC AGCTACGCGC
    GCCCCCCGCA GGGGCGGGCC TTCGGGAACC GAGGGAGCCC ACTGGCTCCC GCGGCTGCGC
    TCTCACTCAC TCTCTCCTTC CCTAGCTGCA GCTAACTCGG GTCGGGAAAA TGCGACAGCG
    ACTGCCCACT CGAGGTCGTC ATGGCGACAG CCAGTGAACG TGTTCCTCTC CTCGGGCAGG
    CCCCCGAGTG TAGAGGAACT GCTTCGCGAG GCGCAGCTCA ATCTCCAGAG CCTGTTGCAA
    GGGCAGCAGT TTGATAGACA TGCAAGTTTT CGACACTCGT TGTTTAACAC AGAGACAGCC
    GTGAACCCCA AGTCCACCCT GAGGCGGAGG CGGACCATTA TTGGATTCTC TAACTTTTCT
    CAGCGAGACC AAGGTCACAG CAGCAGCCCC ACAGGCAGCC TGGCCCGCTC TGCCACCTCA
    GACATCAGGC CTGGCCACTC AGCTCCACAA GTTGTTCAGG GAAGAGTTGC TGTTGGGCAG
    GAAGCTCGGT TCCCAAGTCT CACCTCACCA GGACTGAGAC ACTCTTCTAG TGAGCCCGGA
    GACGCTCACC AGGCACGCAG TGCCTCAGAC CATCCCGGCA TGGAGAGCAT GGCAGTGGTG
    TACAGTGTCC CCAGTTCTTG CAATGGACCA ACAGAGTCAA CATTCTCCAC TTCCTGGAAG
    GGAGATGCTT TCACATACAT GACCCCAAGT GCCAGCAGCC AGGGCAATCA AGTCAGTGAA
    AATGGAAAAA ACCCTTCCTC GGGCAATTCT TGGGTCCCTC TGAACACACT CCCACCTCTG
    GTTCCTAAGG AGGCGGCCAC GCTCTTTGTC ACCCGTGATA ACCCAGCAGG ATGCACTGGG
    CTACCCAGCT ACTCTGAGCA CCCCACTCTA CGAAGACAGA TACCAGAAAG ACCTCCCAAG
    ATTGGCCTTC TTGCCCGTGG TACCTCAAGG CTGGAAACAG GCCCAGGTGG GACCAACAGG
    TTCCGGGAGC GGTCACTGTC TGTACCCACA GACTCGGGTG TTACCTCAGT GGACTATGAC
    GAAGAACAGA AGACCAGTGA GACCCGCATC CTGCCTTATG CCAGTACAAG CTCCGAGGGC
    AGTAACAGTA CTGACAACAT TGCAGCCCTC AGCACTGAGC AGGAGGCACG GCACAGAAGG
    CAAAGATCCA AAAGTATTTC ACTCAAGAAG GCCAAAAAGA AGCCCTCTCC ACCCATGCGA
    AGTGTCTCAC TCGTCAAAGA TGAGCCAGCC CTCCCACCAG AAGGCGAATT GGTACTGCCC
    AAGGACCAGA GGCCTAGGAG CCTTTGCCTC TCCTTGGAAC ACCAAGGGCA CCACCCACCT
    CACCCAGATG CTCAAGGTCA CCCAGCTGTG CCAATGCTCA AAGATCCAGG AAGTACGCAG
    TTCTCTCACC ACTGGTATCT TACTGACTGG AAGTCTGGTG ACACCTACCA GTCCTTGTCC
    AGCTCCAGCA CTGCCACCGG CACCACGGTC ATTGAGTGCA CTCAAGTTCA GGGAAGCTCA
    GAGTCTCTGG CCTCCCCTTC CACCTCCAGA GCGACAACAC CCTCCCAGCT CTCCATTGAG
    GTGGAAGCCA GGGAAGTAGC CTCTCCTGGG AGGCCCACTG GACTGATGTC CCCTTCCAGT
    GGTTACTCCA GCCAGTCAGA GACACCCACA CCCACTGTCT CCATGTCCTT GACCCTGGGC
    CACTTACCTC CTCCAAGTGC TAGTGTCAGA GTACGTCCAG TGGTACCAGA AAGGAAGTCA
    TCACTGCCCC CAACATCACC AATGGAGAAA ATCTGCAAGT CACGGTTATC ATTTGACCTA
    CCACTGACCT CTTCAACCAC CCTGGATCTG TCGGGGATGA GTATCTCCAT CCGCAGCAAA
    ACCAAGGTGA GCCGCCATCA CTCTGATACC AATTTTGGGG TCAAACTGGC ACAGAAAACT
    AGTCCAAACC AACCGATCAT GCCCATGGTT ACTCAGTCTG ACCTCCGTTC TGTTCGCCTG
    AGGTCAGTCA GCAAGTCTGA GCCAGAAGAC GACATTGAGA GTCCAGATTA CATAGAGGAA
    CCTGGAGCAG AGGAAGTCTT CACCATGCCA GAGAGAAAAG TGAAACCTCC CATAGCCGAG
    AAACCTCCAT TGGCCCGAAG GCCTCCAAGT TTGGTCCACA GGCCGCCCTC TCTCCCTGGG
    GAGTATCCAC TAACTTCTCC TACTATGGCT ATGGCATCCA GGAGCTCCAT TCCACACATG
    AAGCAGCTTC CCCAAGACAG CTACACAGTG TTGCGGAAAC CAAAGTCACC CAGCTTCCCC
    GGGGAGTCAA CAGCATCCTC CAGCCTTGTC CTCTCACCTC TTGCCAGTTC CTCTGGTGCT
    TTCTTCTCAG GAACACAGCA ACCTCCCCAG GCCAGTGTAG AAGACGGGGG CCCCAAGGTG
    AGAGCCCTGC CTGAAAGAAT CGGCCTCCAG AGCCAGGAAG AAGCTGAGAA AAAGATGACC
    AAGATTCCAC CTCCGGTACC AAAAAAGCCC AGTGTGCTCT ACCTGCCTCT CACCTCCCCT
    GTAGCTCAAA TGGATGCCTG CATGGCAGAA CCAAGGCTAC CTTTCAGCCC CATCATCACC
    CTGGAGGAAG ATGGCAAGTG TCCCTCCACT GGTGATGACC AGAAGTCACC TGGTAAAGGG
    GTGACTTCGC CTCTTCACAC TGACACAGAA AAAGAGGCCA TCTCTCCAGG GAGGTCAGTG
    GAACCGAGTG CTGAAGAGAA AAGTTTAATC AGTGATAAAA CAGCCGAATG GATTGCAGAG
    GAGGAGGATG ACGTGTTTGT GGCTTCCCGC ACAACTGAAG ATTTATTTAC TGTGATACAC
    AGGTCCAAAA GAAAGCTACT GGGCTGGAAA GAGACTGGGG AGGGCTTCAC GGGAAGCAAA
    CCCAGCTCCC ATTCACCAGT GAAGAATACA GCTGATTCTC CCACCGGTGA GGCTGCTGCT
    GCCCCAGGGC CAAGTAGCAG TGCCTGCCTA GATGCTGGTA GAAATGATGA TTTCAAGGCC
    TTGCTCCAGA AGAAGGGAAG TAAGGCAACT CCCAGGACCC GCCCCTCAGC GGCCGAGCTG
    CTGAAGACCA CTAACCCTCT GGCTCGAAGG ATTATTGCAC AGTTCTCAAA AGACTACGAA
    CCCACTGACA ACCCCAGTAC CTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_030107022.1
    CDS20..3463
    Translation

    Target ORF information:

    RefSeq Version XM_030251162.1
    Organism Mus musculus(house mouse)
    Definition Mus musculus NHS-like 2 (Nhsl2), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_030251162.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGAGGAGGT GGGCGTGGGA GGAGACCAAC GTGCGGCGAC AGGGCGACCC GGCGCAAGCG 
    AGCCCGGGCC ACACCCACAG GGGCTCGGTC CGGGACGCGT TACCTCGTGG CAGGGGTCGG
    AGAGAGAGCA GGCAGCAGCT CGGGACCTGG AGGCGGCAGG CGCAGGAGAC AGACGAGACG
    CTGGCAGAGG TGACGCCAAC AGAGGCAGGC AGAGTAGGAG GGAAGGGACT GCGGCAGGAT
    CTGTCGAGGA AAAATCTTGC AGACAGCGAT CCCTCTCGCC TTTTAAGCTC AGCTACGCGC
    GCCCCCCGCA GGGGCGGGCC TTCGGGAACC GAGGGAGCCC ACTGGCTCCC GCGGCTGCGC
    TCTCACTCAC TCTCTCCTTC CCTAGCTGCA GCTAACTCGG GTCGGGAAAA TGCGACAGCG
    ACTGCCCACT CGAGGTCGTC ATGGCGACAG CCAGTGAACG TGTTCCTCTC CTCGGGCAGG
    CCCCCGAGTG TAGAGGAACT GCTTCGCGAG GCGCAGCTCA ATCTCCAGAG CCTGTTGCAA
    GGGCAGCAGT TTGATAGACA TGCAAGTTTT CGACACTCGT TGTTTAACAC AGAGACAGCC
    GTGAACCCCA AGTCCACCCT GAGGCGGAGG CGGACCATTA TTGGATTCTC TAACTTTTCT
    CAGCGAGACC AAGGTCACAG CAGCAGCCCC ACAGGCAGCC TGGCCCGCTC TGCCACCTCA
    GACATCAGGC CTGGCCACTC AGCTCCACAA GTTGTTCAGG GAAGAGTTGC TGTTGGGCAG
    GAAGCTCGGT TCCCAAGTCT CACCTCACCA GGACTGAGAC ACTCTTCTAG TGAGCCCGGA
    GACGCTCACC AGGCACGCAG TGCCTCAGAC CATCCCGGCA TGGAGAGCAT GGCAGTGGTG
    TACAGTGTCC CCAGTTCTTG CAATGGACCA ACAGAGTCAA CATTCTCCAC TTCCTGGAAG
    GGAGATGCTT TCACATACAT GACCCCAAGT GCCAGCAGCC AGGGCAATCA AGTCAGTGAA
    AATGGAAAAA ACCCTTCCTC GGGCAATTCT TGGGTCCCTC TGAACACACT CCCACCTCTG
    GTTCCTAAGG AGGCGGCCAC GCTCTTTGTC ACCCGTGATA ACCCAGCAGG ATGCACTGGG
    CTACCCAGCT ACTCTGAGCA CCCCACTCTA CGAAGACAGA TACCAGAAAG ACCTCCCAAG
    ATTGGCCTTC TTGCCCGTGG TACCTCAAGG CTGGAAACAG GCCCAGGTGG GACCAACAGG
    TTCCGGGAGC GGTCACTGTC TGTACCCACA GACTCGGGTG TTACCTCAGT GGACTATGAC
    GAAGAACAGA AGACCAGTGA GACCCGCATC CTGCCTTATG CCAGTACAAG CTCCGAGGGC
    AGTAACAGTA CTGACAACAT TGCAGCCCTC AGCACTGAGC AGGAGGCACG GCACAGAAGG
    CAAAGATCCA AAAGTATTTC ACTCAAGAAG GCCAAAAAGA AGCCCTCTCC ACCCATGCGA
    AGTGTCTCAC TCGTCAAAGA TGAGCCAGCC CTCCCACCAG AAGGCGAATT GGTACTGCCC
    AAGGACCAGA GGCCTAGGAG CCTTTGCCTC TCCTTGGAAC ACCAAGGGCA CCACCCACCT
    CACCCAGATG CTCAAGGTCA CCCAGCTGTG CCAATGCTCA AAGATCCAGG AAGTACGCAG
    TTCTCTCACC ACTGGTATCT TACTGACTGG AAGTCTGGTG ACACCTACCA GTCCTTGTCC
    AGCTCCAGCA CTGCCACCGG CACCACGGTC ATTGAGTGCA CTCAAGTTCA GGGAAGCTCA
    GAGTCTCTGG CCTCCCCTTC CACCTCCAGA GCGACAACAC CCTCCCAGCT CTCCATTGAG
    GTGGAAGCCA GGGAAGTAGC CTCTCCTGGG AGGCCCACTG GACTGATGTC CCCTTCCAGT
    GGTTACTCCA GCCAGTCAGA GACACCCACA CCCACTGTCT CCATGTCCTT GACCCTGGGC
    CACTTACCTC CTCCAAGTGC TAGTGTCAGA GTACGTCCAG TGGTACCAGA AAGGAAGTCA
    TCACTGCCCC CAACATCACC AATGGAGAAA ATCTGCAAGT CACGGTTATC ATTTGACCTA
    CCACTGACCT CTTCAACCAC CCTGGATCTG TCGGGGATGA GTATCTCCAT CCGCAGCAAA
    ACCAAGGTGA GCCGCCATCA CTCTGATACC AATTTTGGGG TCAAACTGGC ACAGAAAACT
    AGTCCAAACC AACCGATCAT GCCCATGGTT ACTCAGTCTG ACCTCCGTTC TGTTCGCCTG
    AGGTCAGTCA GCAAGTCTGA GCCAGAAGAC GACATTGAGA GTCCAGATTA CATAGAGGAA
    CCTGGAGCAG AGGAAGTCTT CACCATGCCA GAGAGAAAAG TGAAACCTCC CATAGCCGAG
    AAACCTCCAT TGGCCCGAAG GCCTCCAAGT TTGGTCCACA GGCCGCCCTC TCTCCCTGGG
    GAGTATCCAC TAACTTCTCC TACTATGGCT ATGGCATCCA GGAGCTCCAT TCCACACATG
    AAGCAGCTTC CCCAAGACAG CTACACAGTG TTGCGGAAAC CAAAGTCACC CAGCTTCCCC
    GGGGAGTCAA CAGCATCCTC CAGCCTTGTC CTCTCACCTC TTGCCAGTTC CTCTGGTGCT
    TTCTTCTCAG GAACACAGCA ACCTCCCCAG GCCAGTGTAG AAGACGGGGG CCCCAAGGTG
    AGAGCCCTGC CTGAAAGAAT CGGCCTCCAG AGCCAGGAAG AAGCTGAGAA AAAGATGACC
    AAGATTCCAC CTCCGGTACC AAAAAAGCCC AGTGTGCTCT ACCTGCCTCT CACCTCCCCT
    GTAGCTCAAA TGGATGCCTG CATGGCAGAA CCAAGGCTAC CTTTCAGCCC CATCATCACC
    CTGGAGGAAG ATGGCAAGTG TCCCTCCACT GGTGATGACC AGAAGTCACC TGGTAAAGGG
    GTGACTTCGC CTCTTCACAC TGACACAGAA AAAGAGGCCA TCTCTCCAGG GAGGTCAGTG
    GAACCGAGTG CTGAAGAGAA AAGTTTAATC AGTGATAAAA CAGCCGAATG GATTGCAGAG
    GAGGAGGATG ACGTGTTTGT GGCTTCCCGC ACAACTGAAG ATTTATTTAC TGTGATACAC
    AGGTCCAAAA GAAAGCTACT GGGCTGGAAA GAGACTGGGG AGGGCTTCAC GGGAAGCAAA
    CCCAGCTCCC ATTCACCAGT GAAGAATACA GCTGATTCTC CCACCGGTGA GGCTGCTGCT
    GCCCCAGGGC CAAGTAGCAG TGCCTGCCTA GATGCTGGTA GAAATGATGA TTTCAAGGCC
    TTGCTCCAGA AGAAGGGAAG TAAGGCAACT CCCAGGACCC GCCCCTCAGC GGCCGAGCTG
    CTGAAGACCA CTAACCCTCT GGCTCGAAGG ATTATTGCAC AGTTCTCAAA AGACTACGAA
    CCCACTGACA ACCCCAGTAC CTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu56079
    Clone ID Related Accession (Same CDS sequence) XM_006527720.4 , XM_006527720.3
    Accession Version XM_006527720.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3423bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product NHS-like protein 2 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000086.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006527720.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGGAGAGAG GCGAGTCTCT TACCCTCTTC TGGAGCCGGG GAGCTGCAGC TAACTCGGGT 
    CGGGAAAATG CGACAGCGAC TGCCCACTCG AGGTCGTCAT GGCGACAGCC AGTGAACGTG
    TTCCTCTCCT CGGGCAGGCC CCCGAGTGTA GAGGAACTGC TTCGCGAGGC GCAGCTCAAT
    CTCCAGAGCC TGTTGCAAGA AGAATATGAG GAACAGTATT CGGAGGCCAG ACTTCTGGGG
    CAGACCTTCC GCTCTTCTGA TGGGGCCCCT GAGCCTACTC CCAGCCCAAG GCCCCAGTCT
    GCCAAGCGTC TGGAGTTTGT ACTGATGCCT GCAAAACGGC AGCTGAGCGA GGATGAGACC
    ACGACCCAGG GTGTGAGGGC CCCTGAGGCC TGCCTGAGCC TGTCTACTGC CAACAAGCAA
    AGTGCCTGGA ATGACCCCTT CCCTCTACCC ATCCTAAAGG AGAGGCTCTG GCTTCAGCCC
    TGCTCCACAC AGTCTGACCT TGTGCCAATC AACATCTCTG GGCAGCAGTT TGATAGACAT
    GCAAGTTTTC GACACTCGTT GTTTAACACA GAGACAGCCG TGAACCCCAA GTCCACCCTG
    AGGCGGAGGC GGACCATTAT TGGATTCTCT AACTTTTCTC AGCGAGACCA AGGTCACAGC
    AGCAGCCCCA CAGGCAGCCT GGCCCGCTCT GCCACCTCAG ACATCAGGCC TGGCCACTCA
    GCTCCACAAG TTGTTCAGGG AAGAGTTGCT GTTGGGCAGG AAGCTCGGTT CCCAAGTCTC
    ACCTCACCAG GACTGAGACA CTCTTCTAGT GAGCCCGGAG ACGCTCACCA GGCACGCAGT
    GCCTCAGACC ATCCCGGCAT GGAGAGCATG GCAGTGGTGT ACAGTGTCCC CAGTTCTTGC
    AATGGACCAA CAGAGTCAAC ATTCTCCACT TCCTGGAAGG GAGATGCTTT CACATACATG
    ACCCCAAGTG CCAGCAGCCA GGGCAATCAA GTCAGTGAAA ATGGAAAAAA CCCTTCCTCG
    GGCAATTCTT GGGTCCCTCT GAACACACTC CCACCTCTGG TTCCTAAGGA GGCGGCCACG
    CTCTTTGTCA CCCGTGATAA CCCAGCAGGA TGCACTGGGC TACCCAGCTA CTCTGAGCAC
    CCCACTCTAC GAAGACAGAT ACCAGAAAGA CCTCCCAAGA TTGGCCTTCT TGCCCGTGGT
    ACCTCAAGGC TGGAAACAGG CCCAGGTGGG ACCAACAGGT TCCGGGAGCG GTCACTGTCT
    GTACCCACAG ACTCGGGTGT TACCTCAGTG GACTATGACG AAGAACAGAA GACCAGTGAG
    ACCCGCATCC TGCCTTATGC CAGTACAAGC TCCGAGGGCA GTAACAGTAC TGACAACATT
    GCAGCCCTCA GCACTGAGCA GGAGGCACGG CACAGAAGGC AAAGATCCAA AAGTATTTCA
    CTCAAGAAGG CCAAAAAGAA GCCCTCTCCA CCCATGCGAA GTGTCTCACT CGTCAAAGAT
    GAGCCAGCCC TCCCACCAGA AGGCGAATTG GTACTGCCCA AGGACCAGAG GCCTAGGAGC
    CTTTGCCTCT CCTTGGAACA CCAAGGGCAC CACCCACCTC ACCCAGATGC TCAAGGTCAC
    CCAGCTGTGC CAATGCTCAA AGATCCAGGA AGTACGCAGT TCTCTCACCA CTGGTATCTT
    ACTGACTGGA AGTCTGGTGA CACCTACCAG TCCTTGTCCA GCTCCAGCAC TGCCACCGGC
    ACCACGGTCA TTGAGTGCAC TCAAGTTCAG GGAAGCTCAG AGTCTCTGGC CTCCCCTTCC
    ACCTCCAGAG CGACAACACC CTCCCAGCTC TCCATTGAGG TGGAAGCCAG GGAAGTAGCC
    TCTCCTGGGA GGCCCACTGG ACTGATGTCC CCTTCCAGTG GTTACTCCAG CCAGTCAGAG
    ACACCCACAC CCACTGTCTC CATGTCCTTG ACCCTGGGCC ACTTACCTCC TCCAAGTGCT
    AGTGTCAGAG TACGTCCAGT GGTACCAGAA AGGAAGTCAT CACTGCCCCC AACATCACCA
    ATGGAGAAAA TCTGCAAGTC ACGGTTATCA TTTGACCTAC CACTGACCTC TTCAACCACC
    CTGGATCTGT CGGGGATGAG TATCTCCATC CGCAGCAAAA CCAAGGTGAG CCGCCATCAC
    TCTGATACCA ATTTTGGGGT CAAACTGGCA CAGAAAACTA GTCCAAACCA ACCGATCATG
    CCCATGGTTA CTCAGTCTGA CCTCCGTTCT GTTCGCCTGA GGTCAGTCAG CAAGTCTGAG
    CCAGAAGACG ACATTGAGAG TCCAGATTAC ATAGAGGAAC CTGGAGCAGA GGAAGTCTTC
    ACCATGCCAG AGAGAAAAGT GAAACCTCCC ATAGCCGAGA AACCTCCATT GGCCCGAAGG
    CCTCCAAGTT TGGTCCACAG GCCGCCCTCT CTCCCTGGGG AGTATCCACT AACTTCTCCT
    ACTATGGCTA TGGCATCCAG GAGCTCCATT CCACACATGA AGCAGCTTCC CCAAGACAGC
    TACACAGTGT TGCGGAAACC AAAGTCACCC AGCTTCCCCG GGGAGTCAAC AGCATCCTCC
    AGCCTTGTCC TCTCACCTCT TGCCAGTTCC TCTGGTGCTT TCTTCTCAGG AACACAGCAA
    CCTCCCCAGG CCAGTGTAGA AGACGGGGGC CCCAAGGTGA GAGCCCTGCC TGAAAGAATC
    GGCCTCCAGA GCCAGGAAGA AGCTGAGAAA AAGATGACCA AGATTCCACC TCCGGTACCA
    AAAAAGCCCA GTGTGCTCTA CCTGCCTCTC ACCTCCCCTG TAGCTCAAAT GGATGCCTGC
    ATGGCAGAAC CAAGGCTACC TTTCAGCCCC ATCATCACCC TGGAGGAAGA TGGCAAGTGT
    CCCTCCACTG GTGATGACCA GAAGTCACCT GGTAAAGGGG TGACTTCGCC TCTTCACACT
    GACACAGAAA AAGAGGCCAT CTCTCCAGGG AGGTCAGTGG AACCGAGTGC TGAAGAGAAA
    AGTTTAATCA GTGATAAAAC AGCCGAATGG ATTGCAGAGG AGGAGGATGA CGTGTTTGTG
    GCTTCCCGCA CAACTGAAGA TTTATTTACT GTGATACACA GGTCCAAAAG AAAGCTACTG
    GGCTGGAAAG AGACTGGGGA GGGCTTCACG GGAAGCAAAC CCAGCTCCCA TTCACCAGTG
    AAGAATACAG CTGATTCTCC CACCGGTGAG GCTGCTGCTG CCCCAGGGCC AAGTAGCAGT
    GCCTGCCTAG ATGCTGGTAG AAATGATGAT TTCAAGGCCT TGCTCCAGAA GAAGGGAAGT
    AAGGCAACTC CCAGGACCCG CCCCTCAGCG GCCGAGCTGC TGAAGACCAC TAACCCTCTG
    GCTCGAAGGA TTATTGCACA GTTCTCAAAA GACTACGAAC CCACTGACAA CCCCAGTACC
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006527783.1
    CDS5963..9385
    Translation

    Target ORF information:

    RefSeq Version XM_006527720.4
    Organism Mus musculus(house mouse)
    Definition Mus musculus NHS-like 2 (Nhsl2), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006527720.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGGAGAGAG GCGAGTCTCT TACCCTCTTC TGGAGCCGGG GAGCTGCAGC TAACTCGGGT 
    CGGGAAAATG CGACAGCGAC TGCCCACTCG AGGTCGTCAT GGCGACAGCC AGTGAACGTG
    TTCCTCTCCT CGGGCAGGCC CCCGAGTGTA GAGGAACTGC TTCGCGAGGC GCAGCTCAAT
    CTCCAGAGCC TGTTGCAAGA AGAATATGAG GAACAGTATT CGGAGGCCAG ACTTCTGGGG
    CAGACCTTCC GCTCTTCTGA TGGGGCCCCT GAGCCTACTC CCAGCCCAAG GCCCCAGTCT
    GCCAAGCGTC TGGAGTTTGT ACTGATGCCT GCAAAACGGC AGCTGAGCGA GGATGAGACC
    ACGACCCAGG GTGTGAGGGC CCCTGAGGCC TGCCTGAGCC TGTCTACTGC CAACAAGCAA
    AGTGCCTGGA ATGACCCCTT CCCTCTACCC ATCCTAAAGG AGAGGCTCTG GCTTCAGCCC
    TGCTCCACAC AGTCTGACCT TGTGCCAATC AACATCTCTG GGCAGCAGTT TGATAGACAT
    GCAAGTTTTC GACACTCGTT GTTTAACACA GAGACAGCCG TGAACCCCAA GTCCACCCTG
    AGGCGGAGGC GGACCATTAT TGGATTCTCT AACTTTTCTC AGCGAGACCA AGGTCACAGC
    AGCAGCCCCA CAGGCAGCCT GGCCCGCTCT GCCACCTCAG ACATCAGGCC TGGCCACTCA
    GCTCCACAAG TTGTTCAGGG AAGAGTTGCT GTTGGGCAGG AAGCTCGGTT CCCAAGTCTC
    ACCTCACCAG GACTGAGACA CTCTTCTAGT GAGCCCGGAG ACGCTCACCA GGCACGCAGT
    GCCTCAGACC ATCCCGGCAT GGAGAGCATG GCAGTGGTGT ACAGTGTCCC CAGTTCTTGC
    AATGGACCAA CAGAGTCAAC ATTCTCCACT TCCTGGAAGG GAGATGCTTT CACATACATG
    ACCCCAAGTG CCAGCAGCCA GGGCAATCAA GTCAGTGAAA ATGGAAAAAA CCCTTCCTCG
    GGCAATTCTT GGGTCCCTCT GAACACACTC CCACCTCTGG TTCCTAAGGA GGCGGCCACG
    CTCTTTGTCA CCCGTGATAA CCCAGCAGGA TGCACTGGGC TACCCAGCTA CTCTGAGCAC
    CCCACTCTAC GAAGACAGAT ACCAGAAAGA CCTCCCAAGA TTGGCCTTCT TGCCCGTGGT
    ACCTCAAGGC TGGAAACAGG CCCAGGTGGG ACCAACAGGT TCCGGGAGCG GTCACTGTCT
    GTACCCACAG ACTCGGGTGT TACCTCAGTG GACTATGACG AAGAACAGAA GACCAGTGAG
    ACCCGCATCC TGCCTTATGC CAGTACAAGC TCCGAGGGCA GTAACAGTAC TGACAACATT
    GCAGCCCTCA GCACTGAGCA GGAGGCACGG CACAGAAGGC AAAGATCCAA AAGTATTTCA
    CTCAAGAAGG CCAAAAAGAA GCCCTCTCCA CCCATGCGAA GTGTCTCACT CGTCAAAGAT
    GAGCCAGCCC TCCCACCAGA AGGCGAATTG GTACTGCCCA AGGACCAGAG GCCTAGGAGC
    CTTTGCCTCT CCTTGGAACA CCAAGGGCAC CACCCACCTC ACCCAGATGC TCAAGGTCAC
    CCAGCTGTGC CAATGCTCAA AGATCCAGGA AGTACGCAGT TCTCTCACCA CTGGTATCTT
    ACTGACTGGA AGTCTGGTGA CACCTACCAG TCCTTGTCCA GCTCCAGCAC TGCCACCGGC
    ACCACGGTCA TTGAGTGCAC TCAAGTTCAG GGAAGCTCAG AGTCTCTGGC CTCCCCTTCC
    ACCTCCAGAG CGACAACACC CTCCCAGCTC TCCATTGAGG TGGAAGCCAG GGAAGTAGCC
    TCTCCTGGGA GGCCCACTGG ACTGATGTCC CCTTCCAGTG GTTACTCCAG CCAGTCAGAG
    ACACCCACAC CCACTGTCTC CATGTCCTTG ACCCTGGGCC ACTTACCTCC TCCAAGTGCT
    AGTGTCAGAG TACGTCCAGT GGTACCAGAA AGGAAGTCAT CACTGCCCCC AACATCACCA
    ATGGAGAAAA TCTGCAAGTC ACGGTTATCA TTTGACCTAC CACTGACCTC TTCAACCACC
    CTGGATCTGT CGGGGATGAG TATCTCCATC CGCAGCAAAA CCAAGGTGAG CCGCCATCAC
    TCTGATACCA ATTTTGGGGT CAAACTGGCA CAGAAAACTA GTCCAAACCA ACCGATCATG
    CCCATGGTTA CTCAGTCTGA CCTCCGTTCT GTTCGCCTGA GGTCAGTCAG CAAGTCTGAG
    CCAGAAGACG ACATTGAGAG TCCAGATTAC ATAGAGGAAC CTGGAGCAGA GGAAGTCTTC
    ACCATGCCAG AGAGAAAAGT GAAACCTCCC ATAGCCGAGA AACCTCCATT GGCCCGAAGG
    CCTCCAAGTT TGGTCCACAG GCCGCCCTCT CTCCCTGGGG AGTATCCACT AACTTCTCCT
    ACTATGGCTA TGGCATCCAG GAGCTCCATT CCACACATGA AGCAGCTTCC CCAAGACAGC
    TACACAGTGT TGCGGAAACC AAAGTCACCC AGCTTCCCCG GGGAGTCAAC AGCATCCTCC
    AGCCTTGTCC TCTCACCTCT TGCCAGTTCC TCTGGTGCTT TCTTCTCAGG AACACAGCAA
    CCTCCCCAGG CCAGTGTAGA AGACGGGGGC CCCAAGGTGA GAGCCCTGCC TGAAAGAATC
    GGCCTCCAGA GCCAGGAAGA AGCTGAGAAA AAGATGACCA AGATTCCACC TCCGGTACCA
    AAAAAGCCCA GTGTGCTCTA CCTGCCTCTC ACCTCCCCTG TAGCTCAAAT GGATGCCTGC
    ATGGCAGAAC CAAGGCTACC TTTCAGCCCC ATCATCACCC TGGAGGAAGA TGGCAAGTGT
    CCCTCCACTG GTGATGACCA GAAGTCACCT GGTAAAGGGG TGACTTCGCC TCTTCACACT
    GACACAGAAA AAGAGGCCAT CTCTCCAGGG AGGTCAGTGG AACCGAGTGC TGAAGAGAAA
    AGTTTAATCA GTGATAAAAC AGCCGAATGG ATTGCAGAGG AGGAGGATGA CGTGTTTGTG
    GCTTCCCGCA CAACTGAAGA TTTATTTACT GTGATACACA GGTCCAAAAG AAAGCTACTG
    GGCTGGAAAG AGACTGGGGA GGGCTTCACG GGAAGCAAAC CCAGCTCCCA TTCACCAGTG
    AAGAATACAG CTGATTCTCC CACCGGTGAG GCTGCTGCTG CCCCAGGGCC AAGTAGCAGT
    GCCTGCCTAG ATGCTGGTAG AAATGATGAT TTCAAGGCCT TGCTCCAGAA GAAGGGAAGT
    AAGGCAACTC CCAGGACCCG CCCCTCAGCG GCCGAGCTGC TGAAGACCAC TAACCCTCTG
    GCTCGAAGGA TTATTGCACA GTTCTCAAAA GACTACGAAC CCACTGACAA CCCCAGTACC
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu56080
    Clone ID Related Accession (Same CDS sequence) XM_006527721.3 , XM_006527721.4
    Accession Version XM_006527721.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3408bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product NHS-like protein 2 isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000086.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006527721.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    ATGGCAGCAC AGTCTGGAGC TTGGAATGCT GCAGCTAACT CGGGTCGGGA AAATGCGACA 
    GCGACTGCCC ACTCGAGGTC GTCATGGCGA CAGCCAGTGA ACGTGTTCCT CTCCTCGGGC
    AGGCCCCCGA GTGTAGAGGA ACTGCTTCGC GAGGCGCAGC TCAATCTCCA GAGCCTGTTG
    CAAGAAGAAT ATGAGGAACA GTATTCGGAG GCCAGACTTC TGGGGCAGAC CTTCCGCTCT
    TCTGATGGGG CCCCTGAGCC TACTCCCAGC CCAAGGCCCC AGTCTGCCAA GCGTCTGGAG
    TTTGTACTGA TGCCTGCAAA ACGGCAGCTG AGCGAGGATG AGACCACGAC CCAGGGTGTG
    AGGGCCCCTG AGGCCTGCCT GAGCCTGTCT ACTGCCAACA AGCAAAGTGC CTGGAATGAC
    CCCTTCCCTC TACCCATCCT AAAGGAGAGG CTCTGGCTTC AGCCCTGCTC CACACAGTCT
    GACCTTGTGC CAATCAACAT CTCTGGGCAG CAGTTTGATA GACATGCAAG TTTTCGACAC
    TCGTTGTTTA ACACAGAGAC AGCCGTGAAC CCCAAGTCCA CCCTGAGGCG GAGGCGGACC
    ATTATTGGAT TCTCTAACTT TTCTCAGCGA GACCAAGGTC ACAGCAGCAG CCCCACAGGC
    AGCCTGGCCC GCTCTGCCAC CTCAGACATC AGGCCTGGCC ACTCAGCTCC ACAAGTTGTT
    CAGGGAAGAG TTGCTGTTGG GCAGGAAGCT CGGTTCCCAA GTCTCACCTC ACCAGGACTG
    AGACACTCTT CTAGTGAGCC CGGAGACGCT CACCAGGCAC GCAGTGCCTC AGACCATCCC
    GGCATGGAGA GCATGGCAGT GGTGTACAGT GTCCCCAGTT CTTGCAATGG ACCAACAGAG
    TCAACATTCT CCACTTCCTG GAAGGGAGAT GCTTTCACAT ACATGACCCC AAGTGCCAGC
    AGCCAGGGCA ATCAAGTCAG TGAAAATGGA AAAAACCCTT CCTCGGGCAA TTCTTGGGTC
    CCTCTGAACA CACTCCCACC TCTGGTTCCT AAGGAGGCGG CCACGCTCTT TGTCACCCGT
    GATAACCCAG CAGGATGCAC TGGGCTACCC AGCTACTCTG AGCACCCCAC TCTACGAAGA
    CAGATACCAG AAAGACCTCC CAAGATTGGC CTTCTTGCCC GTGGTACCTC AAGGCTGGAA
    ACAGGCCCAG GTGGGACCAA CAGGTTCCGG GAGCGGTCAC TGTCTGTACC CACAGACTCG
    GGTGTTACCT CAGTGGACTA TGACGAAGAA CAGAAGACCA GTGAGACCCG CATCCTGCCT
    TATGCCAGTA CAAGCTCCGA GGGCAGTAAC AGTACTGACA ACATTGCAGC CCTCAGCACT
    GAGCAGGAGG CACGGCACAG AAGGCAAAGA TCCAAAAGTA TTTCACTCAA GAAGGCCAAA
    AAGAAGCCCT CTCCACCCAT GCGAAGTGTC TCACTCGTCA AAGATGAGCC AGCCCTCCCA
    CCAGAAGGCG AATTGGTACT GCCCAAGGAC CAGAGGCCTA GGAGCCTTTG CCTCTCCTTG
    GAACACCAAG GGCACCACCC ACCTCACCCA GATGCTCAAG GTCACCCAGC TGTGCCAATG
    CTCAAAGATC CAGGAAGTAC GCAGTTCTCT CACCACTGGT ATCTTACTGA CTGGAAGTCT
    GGTGACACCT ACCAGTCCTT GTCCAGCTCC AGCACTGCCA CCGGCACCAC GGTCATTGAG
    TGCACTCAAG TTCAGGGAAG CTCAGAGTCT CTGGCCTCCC CTTCCACCTC CAGAGCGACA
    ACACCCTCCC AGCTCTCCAT TGAGGTGGAA GCCAGGGAAG TAGCCTCTCC TGGGAGGCCC
    ACTGGACTGA TGTCCCCTTC CAGTGGTTAC TCCAGCCAGT CAGAGACACC CACACCCACT
    GTCTCCATGT CCTTGACCCT GGGCCACTTA CCTCCTCCAA GTGCTAGTGT CAGAGTACGT
    CCAGTGGTAC CAGAAAGGAA GTCATCACTG CCCCCAACAT CACCAATGGA GAAAATCTGC
    AAGTCACGGT TATCATTTGA CCTACCACTG ACCTCTTCAA CCACCCTGGA TCTGTCGGGG
    ATGAGTATCT CCATCCGCAG CAAAACCAAG GTGAGCCGCC ATCACTCTGA TACCAATTTT
    GGGGTCAAAC TGGCACAGAA AACTAGTCCA AACCAACCGA TCATGCCCAT GGTTACTCAG
    TCTGACCTCC GTTCTGTTCG CCTGAGGTCA GTCAGCAAGT CTGAGCCAGA AGACGACATT
    GAGAGTCCAG ATTACATAGA GGAACCTGGA GCAGAGGAAG TCTTCACCAT GCCAGAGAGA
    AAAGTGAAAC CTCCCATAGC CGAGAAACCT CCATTGGCCC GAAGGCCTCC AAGTTTGGTC
    CACAGGCCGC CCTCTCTCCC TGGGGAGTAT CCACTAACTT CTCCTACTAT GGCTATGGCA
    TCCAGGAGCT CCATTCCACA CATGAAGCAG CTTCCCCAAG ACAGCTACAC AGTGTTGCGG
    AAACCAAAGT CACCCAGCTT CCCCGGGGAG TCAACAGCAT CCTCCAGCCT TGTCCTCTCA
    CCTCTTGCCA GTTCCTCTGG TGCTTTCTTC TCAGGAACAC AGCAACCTCC CCAGGCCAGT
    GTAGAAGACG GGGGCCCCAA GGTGAGAGCC CTGCCTGAAA GAATCGGCCT CCAGAGCCAG
    GAAGAAGCTG AGAAAAAGAT GACCAAGATT CCACCTCCGG TACCAAAAAA GCCCAGTGTG
    CTCTACCTGC CTCTCACCTC CCCTGTAGCT CAAATGGATG CCTGCATGGC AGAACCAAGG
    CTACCTTTCA GCCCCATCAT CACCCTGGAG GAAGATGGCA AGTGTCCCTC CACTGGTGAT
    GACCAGAAGT CACCTGGTAA AGGGGTGACT TCGCCTCTTC ACACTGACAC AGAAAAAGAG
    GCCATCTCTC CAGGGAGGTC AGTGGAACCG AGTGCTGAAG AGAAAAGTTT AATCAGTGAT
    AAAACAGCCG AATGGATTGC AGAGGAGGAG GATGACGTGT TTGTGGCTTC CCGCACAACT
    GAAGATTTAT TTACTGTGAT ACACAGGTCC AAAAGAAAGC TACTGGGCTG GAAAGAGACT
    GGGGAGGGCT TCACGGGAAG CAAACCCAGC TCCCATTCAC CAGTGAAGAA TACAGCTGAT
    TCTCCCACCG GTGAGGCTGC TGCTGCCCCA GGGCCAAGTA GCAGTGCCTG CCTAGATGCT
    GGTAGAAATG ATGATTTCAA GGCCTTGCTC CAGAAGAAGG GAAGTAAGGC AACTCCCAGG
    ACCCGCCCCT CAGCGGCCGA GCTGCTGAAG ACCACTAACC CTCTGGCTCG AAGGATTATT
    GCACAGTTCT CAAAAGACTA CGAACCCACT GACAACCCCA GTACCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006527784.1
    CDS152..3559
    Translation

    Target ORF information:

    RefSeq Version XM_006527721.4
    Organism Mus musculus(house mouse)
    Definition Mus musculus NHS-like 2 (Nhsl2), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006527721.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    ATGGCAGCAC AGTCTGGAGC TTGGAATGCT GCAGCTAACT CGGGTCGGGA AAATGCGACA 
    GCGACTGCCC ACTCGAGGTC GTCATGGCGA CAGCCAGTGA ACGTGTTCCT CTCCTCGGGC
    AGGCCCCCGA GTGTAGAGGA ACTGCTTCGC GAGGCGCAGC TCAATCTCCA GAGCCTGTTG
    CAAGAAGAAT ATGAGGAACA GTATTCGGAG GCCAGACTTC TGGGGCAGAC CTTCCGCTCT
    TCTGATGGGG CCCCTGAGCC TACTCCCAGC CCAAGGCCCC AGTCTGCCAA GCGTCTGGAG
    TTTGTACTGA TGCCTGCAAA ACGGCAGCTG AGCGAGGATG AGACCACGAC CCAGGGTGTG
    AGGGCCCCTG AGGCCTGCCT GAGCCTGTCT ACTGCCAACA AGCAAAGTGC CTGGAATGAC
    CCCTTCCCTC TACCCATCCT AAAGGAGAGG CTCTGGCTTC AGCCCTGCTC CACACAGTCT
    GACCTTGTGC CAATCAACAT CTCTGGGCAG CAGTTTGATA GACATGCAAG TTTTCGACAC
    TCGTTGTTTA ACACAGAGAC AGCCGTGAAC CCCAAGTCCA CCCTGAGGCG GAGGCGGACC
    ATTATTGGAT TCTCTAACTT TTCTCAGCGA GACCAAGGTC ACAGCAGCAG CCCCACAGGC
    AGCCTGGCCC GCTCTGCCAC CTCAGACATC AGGCCTGGCC ACTCAGCTCC ACAAGTTGTT
    CAGGGAAGAG TTGCTGTTGG GCAGGAAGCT CGGTTCCCAA GTCTCACCTC ACCAGGACTG
    AGACACTCTT CTAGTGAGCC CGGAGACGCT CACCAGGCAC GCAGTGCCTC AGACCATCCC
    GGCATGGAGA GCATGGCAGT GGTGTACAGT GTCCCCAGTT CTTGCAATGG ACCAACAGAG
    TCAACATTCT CCACTTCCTG GAAGGGAGAT GCTTTCACAT ACATGACCCC AAGTGCCAGC
    AGCCAGGGCA ATCAAGTCAG TGAAAATGGA AAAAACCCTT CCTCGGGCAA TTCTTGGGTC
    CCTCTGAACA CACTCCCACC TCTGGTTCCT AAGGAGGCGG CCACGCTCTT TGTCACCCGT
    GATAACCCAG CAGGATGCAC TGGGCTACCC AGCTACTCTG AGCACCCCAC TCTACGAAGA
    CAGATACCAG AAAGACCTCC CAAGATTGGC CTTCTTGCCC GTGGTACCTC AAGGCTGGAA
    ACAGGCCCAG GTGGGACCAA CAGGTTCCGG GAGCGGTCAC TGTCTGTACC CACAGACTCG
    GGTGTTACCT CAGTGGACTA TGACGAAGAA CAGAAGACCA GTGAGACCCG CATCCTGCCT
    TATGCCAGTA CAAGCTCCGA GGGCAGTAAC AGTACTGACA ACATTGCAGC CCTCAGCACT
    GAGCAGGAGG CACGGCACAG AAGGCAAAGA TCCAAAAGTA TTTCACTCAA GAAGGCCAAA
    AAGAAGCCCT CTCCACCCAT GCGAAGTGTC TCACTCGTCA AAGATGAGCC AGCCCTCCCA
    CCAGAAGGCG AATTGGTACT GCCCAAGGAC CAGAGGCCTA GGAGCCTTTG CCTCTCCTTG
    GAACACCAAG GGCACCACCC ACCTCACCCA GATGCTCAAG GTCACCCAGC TGTGCCAATG
    CTCAAAGATC CAGGAAGTAC GCAGTTCTCT CACCACTGGT ATCTTACTGA CTGGAAGTCT
    GGTGACACCT ACCAGTCCTT GTCCAGCTCC AGCACTGCCA CCGGCACCAC GGTCATTGAG
    TGCACTCAAG TTCAGGGAAG CTCAGAGTCT CTGGCCTCCC CTTCCACCTC CAGAGCGACA
    ACACCCTCCC AGCTCTCCAT TGAGGTGGAA GCCAGGGAAG TAGCCTCTCC TGGGAGGCCC
    ACTGGACTGA TGTCCCCTTC CAGTGGTTAC TCCAGCCAGT CAGAGACACC CACACCCACT
    GTCTCCATGT CCTTGACCCT GGGCCACTTA CCTCCTCCAA GTGCTAGTGT CAGAGTACGT
    CCAGTGGTAC CAGAAAGGAA GTCATCACTG CCCCCAACAT CACCAATGGA GAAAATCTGC
    AAGTCACGGT TATCATTTGA CCTACCACTG ACCTCTTCAA CCACCCTGGA TCTGTCGGGG
    ATGAGTATCT CCATCCGCAG CAAAACCAAG GTGAGCCGCC ATCACTCTGA TACCAATTTT
    GGGGTCAAAC TGGCACAGAA AACTAGTCCA AACCAACCGA TCATGCCCAT GGTTACTCAG
    TCTGACCTCC GTTCTGTTCG CCTGAGGTCA GTCAGCAAGT CTGAGCCAGA AGACGACATT
    GAGAGTCCAG ATTACATAGA GGAACCTGGA GCAGAGGAAG TCTTCACCAT GCCAGAGAGA
    AAAGTGAAAC CTCCCATAGC CGAGAAACCT CCATTGGCCC GAAGGCCTCC AAGTTTGGTC
    CACAGGCCGC CCTCTCTCCC TGGGGAGTAT CCACTAACTT CTCCTACTAT GGCTATGGCA
    TCCAGGAGCT CCATTCCACA CATGAAGCAG CTTCCCCAAG ACAGCTACAC AGTGTTGCGG
    AAACCAAAGT CACCCAGCTT CCCCGGGGAG TCAACAGCAT CCTCCAGCCT TGTCCTCTCA
    CCTCTTGCCA GTTCCTCTGG TGCTTTCTTC TCAGGAACAC AGCAACCTCC CCAGGCCAGT
    GTAGAAGACG GGGGCCCCAA GGTGAGAGCC CTGCCTGAAA GAATCGGCCT CCAGAGCCAG
    GAAGAAGCTG AGAAAAAGAT GACCAAGATT CCACCTCCGG TACCAAAAAA GCCCAGTGTG
    CTCTACCTGC CTCTCACCTC CCCTGTAGCT CAAATGGATG CCTGCATGGC AGAACCAAGG
    CTACCTTTCA GCCCCATCAT CACCCTGGAG GAAGATGGCA AGTGTCCCTC CACTGGTGAT
    GACCAGAAGT CACCTGGTAA AGGGGTGACT TCGCCTCTTC ACACTGACAC AGAAAAAGAG
    GCCATCTCTC CAGGGAGGTC AGTGGAACCG AGTGCTGAAG AGAAAAGTTT AATCAGTGAT
    AAAACAGCCG AATGGATTGC AGAGGAGGAG GATGACGTGT TTGTGGCTTC CCGCACAACT
    GAAGATTTAT TTACTGTGAT ACACAGGTCC AAAAGAAAGC TACTGGGCTG GAAAGAGACT
    GGGGAGGGCT TCACGGGAAG CAAACCCAGC TCCCATTCAC CAGTGAAGAA TACAGCTGAT
    TCTCCCACCG GTGAGGCTGC TGCTGCCCCA GGGCCAAGTA GCAGTGCCTG CCTAGATGCT
    GGTAGAAATG ATGATTTCAA GGCCTTGCTC CAGAAGAAGG GAAGTAAGGC AACTCCCAGG
    ACCCGCCCCT CAGCGGCCGA GCTGCTGAAG ACCACTAACC CTCTGGCTCG AAGGATTATT
    GCACAGTTCT CAAAAGACTA CGAACCCACT GACAACCCCA GTACCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu56081
    Clone ID Related Accession (Same CDS sequence) XM_030251163.1
    Accession Version XM_030251163.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3312bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product NHS-like protein 2 isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000086.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    ATGAGGAGGT GGGCGTGGGA GGAGACCAAC GTGCGGCGAC AGGGCGACCC GGCGCAAGCG 
    AGCCCGGGCC ACACCCACAG GGGCTCGGTC CGGGACGCGT TACCTCGTGG CAGGGGTCGG
    AGAGAGAGCA GGCAGCAGCT CGGGACCTGG AGGCGGCAGG CGCAGGAGAC AGACGAGACG
    CTGGCAGAGG TGACGCCAAC AGAGGCAGGC AGAGTAGGAG GGAAGGGACT GCGGCAGGAT
    CTGTCGAGGA AAAATCTTGC AGACAGCGAT CCCTCTCGCC TTTTAAGCTC AGCTACGCGC
    GCCCCCCGCA GGGGCGGGCC TTCGGGAACC GAGGGAGCCC ACTGGCTCCC GCGGCTGCGC
    TCTCACTCAC TCTCTCCTTC CCTAGCTGCA GCTAACTCGG GTCGGGAAAA TGCGACAGCG
    ACTGCCCACT CGAGGTCGTC ATGGCGACAG CCAGTGAACG TGTTCCTCTC CTCGGGCAGG
    CCCCCGAGTG TAGAGGAACT GCTTCGCGAG GCGCAGCTCA ATCTCCAGAG CCTGTTGCAA
    GGTCACAGCA GCAGCCCCAC AGGCAGCCTG GCCCGCTCTG CCACCTCAGA CATCAGGCCT
    GGCCACTCAG CTCCACAAGT TGTTCAGGGA AGAGTTGCTG TTGGGCAGGA AGCTCGGTTC
    CCAAGTCTCA CCTCACCAGG ACTGAGACAC TCTTCTAGTG AGCCCGGAGA CGCTCACCAG
    GCACGCAGTG CCTCAGACCA TCCCGGCATG GAGAGCATGG CAGTGGTGTA CAGTGTCCCC
    AGTTCTTGCA ATGGACCAAC AGAGTCAACA TTCTCCACTT CCTGGAAGGG AGATGCTTTC
    ACATACATGA CCCCAAGTGC CAGCAGCCAG GGCAATCAAG TCAGTGAAAA TGGAAAAAAC
    CCTTCCTCGG GCAATTCTTG GGTCCCTCTG AACACACTCC CACCTCTGGT TCCTAAGGAG
    GCGGCCACGC TCTTTGTCAC CCGTGATAAC CCAGCAGGAT GCACTGGGCT ACCCAGCTAC
    TCTGAGCACC CCACTCTACG AAGACAGATA CCAGAAAGAC CTCCCAAGAT TGGCCTTCTT
    GCCCGTGGTA CCTCAAGGCT GGAAACAGGC CCAGGTGGGA CCAACAGGTT CCGGGAGCGG
    TCACTGTCTG TACCCACAGA CTCGGGTGTT ACCTCAGTGG ACTATGACGA AGAACAGAAG
    ACCAGTGAGA CCCGCATCCT GCCTTATGCC AGTACAAGCT CCGAGGGCAG TAACAGTACT
    GACAACATTG CAGCCCTCAG CACTGAGCAG GAGGCACGGC ACAGAAGGCA AAGATCCAAA
    AGTATTTCAC TCAAGAAGGC CAAAAAGAAG CCCTCTCCAC CCATGCGAAG TGTCTCACTC
    GTCAAAGATG AGCCAGCCCT CCCACCAGAA GGCGAATTGG TACTGCCCAA GGACCAGAGG
    CCTAGGAGCC TTTGCCTCTC CTTGGAACAC CAAGGGCACC ACCCACCTCA CCCAGATGCT
    CAAGGTCACC CAGCTGTGCC AATGCTCAAA GATCCAGGAA GTACGCAGTT CTCTCACCAC
    TGGTATCTTA CTGACTGGAA GTCTGGTGAC ACCTACCAGT CCTTGTCCAG CTCCAGCACT
    GCCACCGGCA CCACGGTCAT TGAGTGCACT CAAGTTCAGG GAAGCTCAGA GTCTCTGGCC
    TCCCCTTCCA CCTCCAGAGC GACAACACCC TCCCAGCTCT CCATTGAGGT GGAAGCCAGG
    GAAGTAGCCT CTCCTGGGAG GCCCACTGGA CTGATGTCCC CTTCCAGTGG TTACTCCAGC
    CAGTCAGAGA CACCCACACC CACTGTCTCC ATGTCCTTGA CCCTGGGCCA CTTACCTCCT
    CCAAGTGCTA GTGTCAGAGT ACGTCCAGTG GTACCAGAAA GGAAGTCATC ACTGCCCCCA
    ACATCACCAA TGGAGAAAAT CTGCAAGTCA CGGTTATCAT TTGACCTACC ACTGACCTCT
    TCAACCACCC TGGATCTGTC GGGGATGAGT ATCTCCATCC GCAGCAAAAC CAAGGTGAGC
    CGCCATCACT CTGATACCAA TTTTGGGGTC AAACTGGCAC AGAAAACTAG TCCAAACCAA
    CCGATCATGC CCATGGTTAC TCAGTCTGAC CTCCGTTCTG TTCGCCTGAG GTCAGTCAGC
    AAGTCTGAGC CAGAAGACGA CATTGAGAGT CCAGATTACA TAGAGGAACC TGGAGCAGAG
    GAAGTCTTCA CCATGCCAGA GAGAAAAGTG AAACCTCCCA TAGCCGAGAA ACCTCCATTG
    GCCCGAAGGC CTCCAAGTTT GGTCCACAGG CCGCCCTCTC TCCCTGGGGA GTATCCACTA
    ACTTCTCCTA CTATGGCTAT GGCATCCAGG AGCTCCATTC CACACATGAA GCAGCTTCCC
    CAAGACAGCT ACACAGTGTT GCGGAAACCA AAGTCACCCA GCTTCCCCGG GGAGTCAACA
    GCATCCTCCA GCCTTGTCCT CTCACCTCTT GCCAGTTCCT CTGGTGCTTT CTTCTCAGGA
    ACACAGCAAC CTCCCCAGGC CAGTGTAGAA GACGGGGGCC CCAAGGTGAG AGCCCTGCCT
    GAAAGAATCG GCCTCCAGAG CCAGGAAGAA GCTGAGAAAA AGATGACCAA GATTCCACCT
    CCGGTACCAA AAAAGCCCAG TGTGCTCTAC CTGCCTCTCA CCTCCCCTGT AGCTCAAATG
    GATGCCTGCA TGGCAGAACC AAGGCTACCT TTCAGCCCCA TCATCACCCT GGAGGAAGAT
    GGCAAGTGTC CCTCCACTGG TGATGACCAG AAGTCACCTG GTAAAGGGGT GACTTCGCCT
    CTTCACACTG ACACAGAAAA AGAGGCCATC TCTCCAGGGA GGTCAGTGGA ACCGAGTGCT
    GAAGAGAAAA GTTTAATCAG TGATAAAACA GCCGAATGGA TTGCAGAGGA GGAGGATGAC
    GTGTTTGTGG CTTCCCGCAC AACTGAAGAT TTATTTACTG TGATACACAG GTCCAAAAGA
    AAGCTACTGG GCTGGAAAGA GACTGGGGAG GGCTTCACGG GAAGCAAACC CAGCTCCCAT
    TCACCAGTGA AGAATACAGC TGATTCTCCC ACCGGTGAGG CTGCTGCTGC CCCAGGGCCA
    AGTAGCAGTG CCTGCCTAGA TGCTGGTAGA AATGATGATT TCAAGGCCTT GCTCCAGAAG
    AAGGGAAGTA AGGCAACTCC CAGGACCCGC CCCTCAGCGG CCGAGCTGCT GAAGACCACT
    AACCCTCTGG CTCGAAGGAT TATTGCACAG TTCTCAAAAG ACTACGAACC CACTGACAAC
    CCCAGTACCT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_030107023.1
    CDS20..3331
    Translation

    Target ORF information:

    RefSeq Version XM_030251163.1
    Organism Mus musculus(house mouse)
    Definition Mus musculus NHS-like 2 (Nhsl2), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_030251163.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    ATGAGGAGGT GGGCGTGGGA GGAGACCAAC GTGCGGCGAC AGGGCGACCC GGCGCAAGCG 
    AGCCCGGGCC ACACCCACAG GGGCTCGGTC CGGGACGCGT TACCTCGTGG CAGGGGTCGG
    AGAGAGAGCA GGCAGCAGCT CGGGACCTGG AGGCGGCAGG CGCAGGAGAC AGACGAGACG
    CTGGCAGAGG TGACGCCAAC AGAGGCAGGC AGAGTAGGAG GGAAGGGACT GCGGCAGGAT
    CTGTCGAGGA AAAATCTTGC AGACAGCGAT CCCTCTCGCC TTTTAAGCTC AGCTACGCGC
    GCCCCCCGCA GGGGCGGGCC TTCGGGAACC GAGGGAGCCC ACTGGCTCCC GCGGCTGCGC
    TCTCACTCAC TCTCTCCTTC CCTAGCTGCA GCTAACTCGG GTCGGGAAAA TGCGACAGCG
    ACTGCCCACT CGAGGTCGTC ATGGCGACAG CCAGTGAACG TGTTCCTCTC CTCGGGCAGG
    CCCCCGAGTG TAGAGGAACT GCTTCGCGAG GCGCAGCTCA ATCTCCAGAG CCTGTTGCAA
    GGTCACAGCA GCAGCCCCAC AGGCAGCCTG GCCCGCTCTG CCACCTCAGA CATCAGGCCT
    GGCCACTCAG CTCCACAAGT TGTTCAGGGA AGAGTTGCTG TTGGGCAGGA AGCTCGGTTC
    CCAAGTCTCA CCTCACCAGG ACTGAGACAC TCTTCTAGTG AGCCCGGAGA CGCTCACCAG
    GCACGCAGTG CCTCAGACCA TCCCGGCATG GAGAGCATGG CAGTGGTGTA CAGTGTCCCC
    AGTTCTTGCA ATGGACCAAC AGAGTCAACA TTCTCCACTT CCTGGAAGGG AGATGCTTTC
    ACATACATGA CCCCAAGTGC CAGCAGCCAG GGCAATCAAG TCAGTGAAAA TGGAAAAAAC
    CCTTCCTCGG GCAATTCTTG GGTCCCTCTG AACACACTCC CACCTCTGGT TCCTAAGGAG
    GCGGCCACGC TCTTTGTCAC CCGTGATAAC CCAGCAGGAT GCACTGGGCT ACCCAGCTAC
    TCTGAGCACC CCACTCTACG AAGACAGATA CCAGAAAGAC CTCCCAAGAT TGGCCTTCTT
    GCCCGTGGTA CCTCAAGGCT GGAAACAGGC CCAGGTGGGA CCAACAGGTT CCGGGAGCGG
    TCACTGTCTG TACCCACAGA CTCGGGTGTT ACCTCAGTGG ACTATGACGA AGAACAGAAG
    ACCAGTGAGA CCCGCATCCT GCCTTATGCC AGTACAAGCT CCGAGGGCAG TAACAGTACT
    GACAACATTG CAGCCCTCAG CACTGAGCAG GAGGCACGGC ACAGAAGGCA AAGATCCAAA
    AGTATTTCAC TCAAGAAGGC CAAAAAGAAG CCCTCTCCAC CCATGCGAAG TGTCTCACTC
    GTCAAAGATG AGCCAGCCCT CCCACCAGAA GGCGAATTGG TACTGCCCAA GGACCAGAGG
    CCTAGGAGCC TTTGCCTCTC CTTGGAACAC CAAGGGCACC ACCCACCTCA CCCAGATGCT
    CAAGGTCACC CAGCTGTGCC AATGCTCAAA GATCCAGGAA GTACGCAGTT CTCTCACCAC
    TGGTATCTTA CTGACTGGAA GTCTGGTGAC ACCTACCAGT CCTTGTCCAG CTCCAGCACT
    GCCACCGGCA CCACGGTCAT TGAGTGCACT CAAGTTCAGG GAAGCTCAGA GTCTCTGGCC
    TCCCCTTCCA CCTCCAGAGC GACAACACCC TCCCAGCTCT CCATTGAGGT GGAAGCCAGG
    GAAGTAGCCT CTCCTGGGAG GCCCACTGGA CTGATGTCCC CTTCCAGTGG TTACTCCAGC
    CAGTCAGAGA CACCCACACC CACTGTCTCC ATGTCCTTGA CCCTGGGCCA CTTACCTCCT
    CCAAGTGCTA GTGTCAGAGT ACGTCCAGTG GTACCAGAAA GGAAGTCATC ACTGCCCCCA
    ACATCACCAA TGGAGAAAAT CTGCAAGTCA CGGTTATCAT TTGACCTACC ACTGACCTCT
    TCAACCACCC TGGATCTGTC GGGGATGAGT ATCTCCATCC GCAGCAAAAC CAAGGTGAGC
    CGCCATCACT CTGATACCAA TTTTGGGGTC AAACTGGCAC AGAAAACTAG TCCAAACCAA
    CCGATCATGC CCATGGTTAC TCAGTCTGAC CTCCGTTCTG TTCGCCTGAG GTCAGTCAGC
    AAGTCTGAGC CAGAAGACGA CATTGAGAGT CCAGATTACA TAGAGGAACC TGGAGCAGAG
    GAAGTCTTCA CCATGCCAGA GAGAAAAGTG AAACCTCCCA TAGCCGAGAA ACCTCCATTG
    GCCCGAAGGC CTCCAAGTTT GGTCCACAGG CCGCCCTCTC TCCCTGGGGA GTATCCACTA
    ACTTCTCCTA CTATGGCTAT GGCATCCAGG AGCTCCATTC CACACATGAA GCAGCTTCCC
    CAAGACAGCT ACACAGTGTT GCGGAAACCA AAGTCACCCA GCTTCCCCGG GGAGTCAACA
    GCATCCTCCA GCCTTGTCCT CTCACCTCTT GCCAGTTCCT CTGGTGCTTT CTTCTCAGGA
    ACACAGCAAC CTCCCCAGGC CAGTGTAGAA GACGGGGGCC CCAAGGTGAG AGCCCTGCCT
    GAAAGAATCG GCCTCCAGAG CCAGGAAGAA GCTGAGAAAA AGATGACCAA GATTCCACCT
    CCGGTACCAA AAAAGCCCAG TGTGCTCTAC CTGCCTCTCA CCTCCCCTGT AGCTCAAATG
    GATGCCTGCA TGGCAGAACC AAGGCTACCT TTCAGCCCCA TCATCACCCT GGAGGAAGAT
    GGCAAGTGTC CCTCCACTGG TGATGACCAG AAGTCACCTG GTAAAGGGGT GACTTCGCCT
    CTTCACACTG ACACAGAAAA AGAGGCCATC TCTCCAGGGA GGTCAGTGGA ACCGAGTGCT
    GAAGAGAAAA GTTTAATCAG TGATAAAACA GCCGAATGGA TTGCAGAGGA GGAGGATGAC
    GTGTTTGTGG CTTCCCGCAC AACTGAAGAT TTATTTACTG TGATACACAG GTCCAAAAGA
    AAGCTACTGG GCTGGAAAGA GACTGGGGAG GGCTTCACGG GAAGCAAACC CAGCTCCCAT
    TCACCAGTGA AGAATACAGC TGATTCTCCC ACCGGTGAGG CTGCTGCTGC CCCAGGGCCA
    AGTAGCAGTG CCTGCCTAGA TGCTGGTAGA AATGATGATT TCAAGGCCTT GCTCCAGAAG
    AAGGGAAGTA AGGCAACTCC CAGGACCCGC CCCTCAGCGG CCGAGCTGCT GAAGACCACT
    AACCCTCTGG CTCGAAGGAT TATTGCACAG TTCTCAAAAG ACTACGAACC CACTGACAAC
    CCCAGTACCT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu56082
    Clone ID Related Accession (Same CDS sequence) XM_006527723.4 , XM_006527723.3
    Accession Version XM_006527723.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3099bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product NHS-like protein 2 isoform X11
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000086.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006527723.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    ATGCCTGCAA AACGGCAGCT GAGCGAGGAT GAGACCACGA CCCAGGGTGT GAGGGCCCCT 
    GAGGCCTGCC TGAGCCTGTC TACTGCCAAC AAGCAAAGTG CCTGGAATGA CCCCTTCCCT
    CTACCCATCC TAAAGGAGAG GCTCTGGCTT CAGCCCTGCT CCACACAGTC TGACCTTGTG
    CCAATCAACA TCTCTGGGCA GCAGTTTGAT AGACATGCAA GTTTTCGACA CTCGTTGTTT
    AACACAGAGA CAGCCGTGAA CCCCAAGTCC ACCCTGAGGC GGAGGCGGAC CATTATTGGA
    TTCTCTAACT TTTCTCAGCG AGACCAAGGT CACAGCAGCA GCCCCACAGG CAGCCTGGCC
    CGCTCTGCCA CCTCAGACAT CAGGCCTGGC CACTCAGCTC CACAAGTTGT TCAGGGAAGA
    GTTGCTGTTG GGCAGGAAGC TCGGTTCCCA AGTCTCACCT CACCAGGACT GAGACACTCT
    TCTAGTGAGC CCGGAGACGC TCACCAGGCA CGCAGTGCCT CAGACCATCC CGGCATGGAG
    AGCATGGCAG TGGTGTACAG TGTCCCCAGT TCTTGCAATG GACCAACAGA GTCAACATTC
    TCCACTTCCT GGAAGGGAGA TGCTTTCACA TACATGACCC CAAGTGCCAG CAGCCAGGGC
    AATCAAGTCA GTGAAAATGG AAAAAACCCT TCCTCGGGCA ATTCTTGGGT CCCTCTGAAC
    ACACTCCCAC CTCTGGTTCC TAAGGAGGCG GCCACGCTCT TTGTCACCCG TGATAACCCA
    GCAGGATGCA CTGGGCTACC CAGCTACTCT GAGCACCCCA CTCTACGAAG ACAGATACCA
    GAAAGACCTC CCAAGATTGG CCTTCTTGCC CGTGGTACCT CAAGGCTGGA AACAGGCCCA
    GGTGGGACCA ACAGGTTCCG GGAGCGGTCA CTGTCTGTAC CCACAGACTC GGGTGTTACC
    TCAGTGGACT ATGACGAAGA ACAGAAGACC AGTGAGACCC GCATCCTGCC TTATGCCAGT
    ACAAGCTCCG AGGGCAGTAA CAGTACTGAC AACATTGCAG CCCTCAGCAC TGAGCAGGAG
    GCACGGCACA GAAGGCAAAG ATCCAAAAGT ATTTCACTCA AGAAGGCCAA AAAGAAGCCC
    TCTCCACCCA TGCGAAGTGT CTCACTCGTC AAAGATGAGC CAGCCCTCCC ACCAGAAGGC
    GAATTGGTAC TGCCCAAGGA CCAGAGGCCT AGGAGCCTTT GCCTCTCCTT GGAACACCAA
    GGGCACCACC CACCTCACCC AGATGCTCAA GGTCACCCAG CTGTGCCAAT GCTCAAAGAT
    CCAGGAAGTA CGCAGTTCTC TCACCACTGG TATCTTACTG ACTGGAAGTC TGGTGACACC
    TACCAGTCCT TGTCCAGCTC CAGCACTGCC ACCGGCACCA CGGTCATTGA GTGCACTCAA
    GTTCAGGGAA GCTCAGAGTC TCTGGCCTCC CCTTCCACCT CCAGAGCGAC AACACCCTCC
    CAGCTCTCCA TTGAGGTGGA AGCCAGGGAA GTAGCCTCTC CTGGGAGGCC CACTGGACTG
    ATGTCCCCTT CCAGTGGTTA CTCCAGCCAG TCAGAGACAC CCACACCCAC TGTCTCCATG
    TCCTTGACCC TGGGCCACTT ACCTCCTCCA AGTGCTAGTG TCAGAGTACG TCCAGTGGTA
    CCAGAAAGGA AGTCATCACT GCCCCCAACA TCACCAATGG AGAAAATCTG CAAGTCACGG
    TTATCATTTG ACCTACCACT GACCTCTTCA ACCACCCTGG ATCTGTCGGG GATGAGTATC
    TCCATCCGCA GCAAAACCAA GGTGAGCCGC CATCACTCTG ATACCAATTT TGGGGTCAAA
    CTGGCACAGA AAACTAGTCC AAACCAACCG ATCATGCCCA TGGTTACTCA GTCTGACCTC
    CGTTCTGTTC GCCTGAGGTC AGTCAGCAAG TCTGAGCCAG AAGACGACAT TGAGAGTCCA
    GATTACATAG AGGAACCTGG AGCAGAGGAA GTCTTCACCA TGCCAGAGAG AAAAGTGAAA
    CCTCCCATAG CCGAGAAACC TCCATTGGCC CGAAGGCCTC CAAGTTTGGT CCACAGGCCG
    CCCTCTCTCC CTGGGGAGTA TCCACTAACT TCTCCTACTA TGGCTATGGC ATCCAGGAGC
    TCCATTCCAC ACATGAAGCA GCTTCCCCAA GACAGCTACA CAGTGTTGCG GAAACCAAAG
    TCACCCAGCT TCCCCGGGGA GTCAACAGCA TCCTCCAGCC TTGTCCTCTC ACCTCTTGCC
    AGTTCCTCTG GTGCTTTCTT CTCAGGAACA CAGCAACCTC CCCAGGCCAG TGTAGAAGAC
    GGGGGCCCCA AGGTGAGAGC CCTGCCTGAA AGAATCGGCC TCCAGAGCCA GGAAGAAGCT
    GAGAAAAAGA TGACCAAGAT TCCACCTCCG GTACCAAAAA AGCCCAGTGT GCTCTACCTG
    CCTCTCACCT CCCCTGTAGC TCAAATGGAT GCCTGCATGG CAGAACCAAG GCTACCTTTC
    AGCCCCATCA TCACCCTGGA GGAAGATGGC AAGTGTCCCT CCACTGGTGA TGACCAGAAG
    TCACCTGGTA AAGGGGTGAC TTCGCCTCTT CACACTGACA CAGAAAAAGA GGCCATCTCT
    CCAGGGAGGT CAGTGGAACC GAGTGCTGAA GAGAAAAGTT TAATCAGTGA TAAAACAGCC
    GAATGGATTG CAGAGGAGGA GGATGACGTG TTTGTGGCTT CCCGCACAAC TGAAGATTTA
    TTTACTGTGA TACACAGGTC CAAAAGAAAG CTACTGGGCT GGAAAGAGAC TGGGGAGGGC
    TTCACGGGAA GCAAACCCAG CTCCCATTCA CCAGTGAAGA ATACAGCTGA TTCTCCCACC
    GGTGAGGCTG CTGCTGCCCC AGGGCCAAGT AGCAGTGCCT GCCTAGATGC TGGTAGAAAT
    GATGATTTCA AGGCCTTGCT CCAGAAGAAG GGAAGTAAGG CAACTCCCAG GACCCGCCCC
    TCAGCGGCCG AGCTGCTGAA GACCACTAAC CCTCTGGCTC GAAGGATTAT TGCACAGTTC
    TCAAAAGACT ACGAACCCAC TGACAACCCC AGTACCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006527786.1
    CDS297..3395
    Translation

    Target ORF information:

    RefSeq Version XM_006527723.4
    Organism Mus musculus(house mouse)
    Definition Mus musculus NHS-like 2 (Nhsl2), transcript variant X11, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006527723.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    ATGCCTGCAA AACGGCAGCT GAGCGAGGAT GAGACCACGA CCCAGGGTGT GAGGGCCCCT 
    GAGGCCTGCC TGAGCCTGTC TACTGCCAAC AAGCAAAGTG CCTGGAATGA CCCCTTCCCT
    CTACCCATCC TAAAGGAGAG GCTCTGGCTT CAGCCCTGCT CCACACAGTC TGACCTTGTG
    CCAATCAACA TCTCTGGGCA GCAGTTTGAT AGACATGCAA GTTTTCGACA CTCGTTGTTT
    AACACAGAGA CAGCCGTGAA CCCCAAGTCC ACCCTGAGGC GGAGGCGGAC CATTATTGGA
    TTCTCTAACT TTTCTCAGCG AGACCAAGGT CACAGCAGCA GCCCCACAGG CAGCCTGGCC
    CGCTCTGCCA CCTCAGACAT CAGGCCTGGC CACTCAGCTC CACAAGTTGT TCAGGGAAGA
    GTTGCTGTTG GGCAGGAAGC TCGGTTCCCA AGTCTCACCT CACCAGGACT GAGACACTCT
    TCTAGTGAGC CCGGAGACGC TCACCAGGCA CGCAGTGCCT CAGACCATCC CGGCATGGAG
    AGCATGGCAG TGGTGTACAG TGTCCCCAGT TCTTGCAATG GACCAACAGA GTCAACATTC
    TCCACTTCCT GGAAGGGAGA TGCTTTCACA TACATGACCC CAAGTGCCAG CAGCCAGGGC
    AATCAAGTCA GTGAAAATGG AAAAAACCCT TCCTCGGGCA ATTCTTGGGT CCCTCTGAAC
    ACACTCCCAC CTCTGGTTCC TAAGGAGGCG GCCACGCTCT TTGTCACCCG TGATAACCCA
    GCAGGATGCA CTGGGCTACC CAGCTACTCT GAGCACCCCA CTCTACGAAG ACAGATACCA
    GAAAGACCTC CCAAGATTGG CCTTCTTGCC CGTGGTACCT CAAGGCTGGA AACAGGCCCA
    GGTGGGACCA ACAGGTTCCG GGAGCGGTCA CTGTCTGTAC CCACAGACTC GGGTGTTACC
    TCAGTGGACT ATGACGAAGA ACAGAAGACC AGTGAGACCC GCATCCTGCC TTATGCCAGT
    ACAAGCTCCG AGGGCAGTAA CAGTACTGAC AACATTGCAG CCCTCAGCAC TGAGCAGGAG
    GCACGGCACA GAAGGCAAAG ATCCAAAAGT ATTTCACTCA AGAAGGCCAA AAAGAAGCCC
    TCTCCACCCA TGCGAAGTGT CTCACTCGTC AAAGATGAGC CAGCCCTCCC ACCAGAAGGC
    GAATTGGTAC TGCCCAAGGA CCAGAGGCCT AGGAGCCTTT GCCTCTCCTT GGAACACCAA
    GGGCACCACC CACCTCACCC AGATGCTCAA GGTCACCCAG CTGTGCCAAT GCTCAAAGAT
    CCAGGAAGTA CGCAGTTCTC TCACCACTGG TATCTTACTG ACTGGAAGTC TGGTGACACC
    TACCAGTCCT TGTCCAGCTC CAGCACTGCC ACCGGCACCA CGGTCATTGA GTGCACTCAA
    GTTCAGGGAA GCTCAGAGTC TCTGGCCTCC CCTTCCACCT CCAGAGCGAC AACACCCTCC
    CAGCTCTCCA TTGAGGTGGA AGCCAGGGAA GTAGCCTCTC CTGGGAGGCC CACTGGACTG
    ATGTCCCCTT CCAGTGGTTA CTCCAGCCAG TCAGAGACAC CCACACCCAC TGTCTCCATG
    TCCTTGACCC TGGGCCACTT ACCTCCTCCA AGTGCTAGTG TCAGAGTACG TCCAGTGGTA
    CCAGAAAGGA AGTCATCACT GCCCCCAACA TCACCAATGG AGAAAATCTG CAAGTCACGG
    TTATCATTTG ACCTACCACT GACCTCTTCA ACCACCCTGG ATCTGTCGGG GATGAGTATC
    TCCATCCGCA GCAAAACCAA GGTGAGCCGC CATCACTCTG ATACCAATTT TGGGGTCAAA
    CTGGCACAGA AAACTAGTCC AAACCAACCG ATCATGCCCA TGGTTACTCA GTCTGACCTC
    CGTTCTGTTC GCCTGAGGTC AGTCAGCAAG TCTGAGCCAG AAGACGACAT TGAGAGTCCA
    GATTACATAG AGGAACCTGG AGCAGAGGAA GTCTTCACCA TGCCAGAGAG AAAAGTGAAA
    CCTCCCATAG CCGAGAAACC TCCATTGGCC CGAAGGCCTC CAAGTTTGGT CCACAGGCCG
    CCCTCTCTCC CTGGGGAGTA TCCACTAACT TCTCCTACTA TGGCTATGGC ATCCAGGAGC
    TCCATTCCAC ACATGAAGCA GCTTCCCCAA GACAGCTACA CAGTGTTGCG GAAACCAAAG
    TCACCCAGCT TCCCCGGGGA GTCAACAGCA TCCTCCAGCC TTGTCCTCTC ACCTCTTGCC
    AGTTCCTCTG GTGCTTTCTT CTCAGGAACA CAGCAACCTC CCCAGGCCAG TGTAGAAGAC
    GGGGGCCCCA AGGTGAGAGC CCTGCCTGAA AGAATCGGCC TCCAGAGCCA GGAAGAAGCT
    GAGAAAAAGA TGACCAAGAT TCCACCTCCG GTACCAAAAA AGCCCAGTGT GCTCTACCTG
    CCTCTCACCT CCCCTGTAGC TCAAATGGAT GCCTGCATGG CAGAACCAAG GCTACCTTTC
    AGCCCCATCA TCACCCTGGA GGAAGATGGC AAGTGTCCCT CCACTGGTGA TGACCAGAAG
    TCACCTGGTA AAGGGGTGAC TTCGCCTCTT CACACTGACA CAGAAAAAGA GGCCATCTCT
    CCAGGGAGGT CAGTGGAACC GAGTGCTGAA GAGAAAAGTT TAATCAGTGA TAAAACAGCC
    GAATGGATTG CAGAGGAGGA GGATGACGTG TTTGTGGCTT CCCGCACAAC TGAAGATTTA
    TTTACTGTGA TACACAGGTC CAAAAGAAAG CTACTGGGCT GGAAAGAGAC TGGGGAGGGC
    TTCACGGGAA GCAAACCCAG CTCCCATTCA CCAGTGAAGA ATACAGCTGA TTCTCCCACC
    GGTGAGGCTG CTGCTGCCCC AGGGCCAAGT AGCAGTGCCT GCCTAGATGC TGGTAGAAAT
    GATGATTTCA AGGCCTTGCT CCAGAAGAAG GGAAGTAAGG CAACTCCCAG GACCCGCCCC
    TCAGCGGCCG AGCTGCTGAA GACCACTAAC CCTCTGGCTC GAAGGATTAT TGCACAGTTC
    TCAAAAGACT ACGAACCCAC TGACAACCCC AGTACCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu75773
    Clone ID Related Accession (Same CDS sequence) XM_011247499.2 , XM_011247499.1
    Accession Version XM_011247499.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3729bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product NHS-like protein 2 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000086.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_011247499.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGCCGTTCT ACCGGCGCAC TGTGGTGCCC CAGCGTCTGT GCCCGCGCAA CCCTCCGCAG 
    CCGCTGGCTG AGCTCCGCGA CGTGAGCCAC CTGGCCGCGC TCAGTCTGCT CCGCCAGCTC
    GCAGACCTCT GCGGCCACTC GCTGGCTCTG CTGGAGGACC TCGAGGGGCA CCTGCTGGCT
    CTGGGGCGCC GCACCGACAG CCTGTACCGG CGCACCGTCC GGCTCCGCCG CCGCCTTCCC
    TGTCGCCTGC TTGGCCCGGA GGATGATGAA GAATTGCTAG GTGCCTGCCA CTGGGTGAAC
    GGGACTCGCA TCCTGAGGGC AAGGGAGCTT GCTGATGTAG CCAGCACAGC TGCAGCTAAC
    TCGGGTCGGG AAAATGCGAC AGCGACTGCC CACTCGAGGT CGTCATGGCG ACAGCCAGTG
    AACGTGTTCC TCTCCTCGGG CAGGCCCCCG AGTGTAGAGG AACTGCTTCG CGAGGCGCAG
    CTCAATCTCC AGAGCCTGTT GCAAGAAGAA TATGAGGAAC AGTATTCGGA GGCCAGACTT
    CTGGGGCAGA CCTTCCGCTC TTCTGATGGG GCCCCTGAGC CTACTCCCAG CCCAAGGCCC
    CAGTCTGCCA AGCGTCTGGA GTTTGTACTG ATGCCTGCAA AACGGCAGCT GAGCGAGGAT
    GAGACCACGA CCCAGGGTGT GAGGGCCCCT GAGGCCTGCC TGAGCCTGTC TACTGCCAAC
    AAGCAAAGTG CCTGGAATGA CCCCTTCCCT CTACCCATCC TAAAGGAGAG GCTCTGGCTT
    CAGCCCTGCT CCACACAGTC TGACCTTGTG CCAATCAACA TCTCTGGGCA GCAGTTTGAT
    AGACATGCAA GTTTTCGACA CTCGTTGTTT AACACAGAGA CAGCCGTGAA CCCCAAGTCC
    ACCCTGAGGC GGAGGCGGAC CATTATTGGA TTCTCTAACT TTTCTCAGCG AGACCAAGGT
    CACAGCAGCA GCCCCACAGG CAGCCTGGCC CGCTCTGCCA CCTCAGACAT CAGGCCTGGC
    CACTCAGCTC CACAAGTTGT TCAGGGAAGA GTTGCTGTTG GGCAGGAAGC TCGGTTCCCA
    AGTCTCACCT CACCAGGACT GAGACACTCT TCTAGTGAGC CCGGAGACGC TCACCAGGCA
    CGCAGTGCCT CAGACCATCC CGGCATGGAG AGCATGGCAG TGGTGTACAG TGTCCCCAGT
    TCTTGCAATG GACCAACAGA GTCAACATTC TCCACTTCCT GGAAGGGAGA TGCTTTCACA
    TACATGACCC CAAGTGCCAG CAGCCAGGGC AATCAAGTCA GTGAAAATGG AAAAAACCCT
    TCCTCGGGCA ATTCTTGGGT CCCTCTGAAC ACACTCCCAC CTCTGGTTCC TAAGGAGGCG
    GCCACGCTCT TTGTCACCCG TGATAACCCA GCAGGATGCA CTGGGCTACC CAGCTACTCT
    GAGCACCCCA CTCTACGAAG ACAGATACCA GAAAGACCTC CCAAGATTGG CCTTCTTGCC
    CGTGGTACCT CAAGGCTGGA AACAGGCCCA GGTGGGACCA ACAGGTTCCG GGAGCGGTCA
    CTGTCTGTAC CCACAGACTC GGGTGTTACC TCAGTGGACT ATGACGAAGA ACAGAAGACC
    AGTGAGACCC GCATCCTGCC TTATGCCAGT ACAAGCTCCG AGGGCAGTAA CAGTACTGAC
    AACATTGCAG CCCTCAGCAC TGAGCAGGAG GCACGGCACA GAAGGCAAAG ATCCAAAAGT
    ATTTCACTCA AGAAGGCCAA AAAGAAGCCC TCTCCACCCA TGCGAAGTGT CTCACTCGTC
    AAAGATGAGC CAGCCCTCCC ACCAGAAGGC GAATTGGTAC TGCCCAAGGA CCAGAGGCCT
    AGGAGCCTTT GCCTCTCCTT GGAACACCAA GGGCACCACC CACCTCACCC AGATGCTCAA
    GGTCACCCAG CTGTGCCAAT GCTCAAAGAT CCAGGAAGTA CGCAGTTCTC TCACCACTGG
    TATCTTACTG ACTGGAAGTC TGGTGACACC TACCAGTCCT TGTCCAGCTC CAGCACTGCC
    ACCGGCACCA CGGTCATTGA GTGCACTCAA GTTCAGGGAA GCTCAGAGTC TCTGGCCTCC
    CCTTCCACCT CCAGAGCGAC AACACCCTCC CAGCTCTCCA TTGAGGTGGA AGCCAGGGAA
    GTAGCCTCTC CTGGGAGGCC CACTGGACTG ATGTCCCCTT CCAGTGGTTA CTCCAGCCAG
    TCAGAGACAC CCACACCCAC TGTCTCCATG TCCTTGACCC TGGGCCACTT ACCTCCTCCA
    AGTGCTAGTG TCAGAGTACG TCCAGTGGTA CCAGAAAGGA AGTCATCACT GCCCCCAACA
    TCACCAATGG AGAAAATCTG CAAGTCACGG TTATCATTTG ACCTACCACT GACCTCTTCA
    ACCACCCTGG ATCTGTCGGG GATGAGTATC TCCATCCGCA GCAAAACCAA GGTGAGCCGC
    CATCACTCTG ATACCAATTT TGGGGTCAAA CTGGCACAGA AAACTAGTCC AAACCAACCG
    ATCATGCCCA TGGTTACTCA GTCTGACCTC CGTTCTGTTC GCCTGAGGTC AGTCAGCAAG
    TCTGAGCCAG AAGACGACAT TGAGAGTCCA GATTACATAG AGGAACCTGG AGCAGAGGAA
    GTCTTCACCA TGCCAGAGAG AAAAGTGAAA CCTCCCATAG CCGAGAAACC TCCATTGGCC
    CGAAGGCCTC CAAGTTTGGT CCACAGGCCG CCCTCTCTCC CTGGGGAGTA TCCACTAACT
    TCTCCTACTA TGGCTATGGC ATCCAGGAGC TCCATTCCAC ACATGAAGCA GCTTCCCCAA
    GACAGCTACA CAGTGTTGCG GAAACCAAAG TCACCCAGCT TCCCCGGGGA GTCAACAGCA
    TCCTCCAGCC TTGTCCTCTC ACCTCTTGCC AGTTCCTCTG GTGCTTTCTT CTCAGGAACA
    CAGCAACCTC CCCAGGCCAG TGTAGAAGAC GGGGGCCCCA AGGTGAGAGC CCTGCCTGAA
    AGAATCGGCC TCCAGAGCCA GGAAGAAGCT GAGAAAAAGA TGACCAAGAT TCCACCTCCG
    GTACCAAAAA AGCCCAGTGT GCTCTACCTG CCTCTCACCT CCCCTGTAGC TCAAATGGAT
    GCCTGCATGG CAGAACCAAG GCTACCTTTC AGCCCCATCA TCACCCTGGA GGAAGATGGC
    AAGTGTCCCT CCACTGGTGA TGACCAGAAG TCACCTGGTA AAGGGGTGAC TTCGCCTCTT
    CACACTGACA CAGAAAAAGA GGCCATCTCT CCAGGGAGGT CAGTGGAACC GAGTGCTGAA
    GAGAAAAGTT TAATCAGTGA TAAAACAGCC GAATGGATTG CAGAGGAGGA GGATGACGTG
    TTTGTGGCTT CCCGCACAAC TGAAGATTTA TTTACTGTGA TACACAGGTC CAAAAGAAAG
    CTACTGGGCT GGAAAGAGAC TGGGGAGGGC TTCACGGGAA GCAAACCCAG CTCCCATTCA
    CCAGTGAAGA ATACAGCTGA TTCTCCCACC GGTGAGGCTG CTGCTGCCCC AGGGCCAAGT
    AGCAGTGCCT GCCTAGATGC TGGTAGAAAT GATGATTTCA AGGCCTTGCT CCAGAAGAAG
    GGAAGTAAGG CAACTCCCAG GACCCGCCCC TCAGCGGCCG AGCTGCTGAA GACCACTAAC
    CCTCTGGCTC GAAGGATTAT TGCACAGTTC TCAAAAGACT ACGAACCCAC TGACAACCCC
    AGTACCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011245801.1
    CDS308..4036
    Translation

    Target ORF information:

    RefSeq Version XM_011247499.2
    Organism Mus musculus(house mouse)
    Definition Mus musculus NHS-like 2 (Nhsl2), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011247499.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGCCGTTCT ACCGGCGCAC TGTGGTGCCC CAGCGTCTGT GCCCGCGCAA CCCTCCGCAG 
    CCGCTGGCTG AGCTCCGCGA CGTGAGCCAC CTGGCCGCGC TCAGTCTGCT CCGCCAGCTC
    GCAGACCTCT GCGGCCACTC GCTGGCTCTG CTGGAGGACC TCGAGGGGCA CCTGCTGGCT
    CTGGGGCGCC GCACCGACAG CCTGTACCGG CGCACCGTCC GGCTCCGCCG CCGCCTTCCC
    TGTCGCCTGC TTGGCCCGGA GGATGATGAA GAATTGCTAG GTGCCTGCCA CTGGGTGAAC
    GGGACTCGCA TCCTGAGGGC AAGGGAGCTT GCTGATGTAG CCAGCACAGC TGCAGCTAAC
    TCGGGTCGGG AAAATGCGAC AGCGACTGCC CACTCGAGGT CGTCATGGCG ACAGCCAGTG
    AACGTGTTCC TCTCCTCGGG CAGGCCCCCG AGTGTAGAGG AACTGCTTCG CGAGGCGCAG
    CTCAATCTCC AGAGCCTGTT GCAAGAAGAA TATGAGGAAC AGTATTCGGA GGCCAGACTT
    CTGGGGCAGA CCTTCCGCTC TTCTGATGGG GCCCCTGAGC CTACTCCCAG CCCAAGGCCC
    CAGTCTGCCA AGCGTCTGGA GTTTGTACTG ATGCCTGCAA AACGGCAGCT GAGCGAGGAT
    GAGACCACGA CCCAGGGTGT GAGGGCCCCT GAGGCCTGCC TGAGCCTGTC TACTGCCAAC
    AAGCAAAGTG CCTGGAATGA CCCCTTCCCT CTACCCATCC TAAAGGAGAG GCTCTGGCTT
    CAGCCCTGCT CCACACAGTC TGACCTTGTG CCAATCAACA TCTCTGGGCA GCAGTTTGAT
    AGACATGCAA GTTTTCGACA CTCGTTGTTT AACACAGAGA CAGCCGTGAA CCCCAAGTCC
    ACCCTGAGGC GGAGGCGGAC CATTATTGGA TTCTCTAACT TTTCTCAGCG AGACCAAGGT
    CACAGCAGCA GCCCCACAGG CAGCCTGGCC CGCTCTGCCA CCTCAGACAT CAGGCCTGGC
    CACTCAGCTC CACAAGTTGT TCAGGGAAGA GTTGCTGTTG GGCAGGAAGC TCGGTTCCCA
    AGTCTCACCT CACCAGGACT GAGACACTCT TCTAGTGAGC CCGGAGACGC TCACCAGGCA
    CGCAGTGCCT CAGACCATCC CGGCATGGAG AGCATGGCAG TGGTGTACAG TGTCCCCAGT
    TCTTGCAATG GACCAACAGA GTCAACATTC TCCACTTCCT GGAAGGGAGA TGCTTTCACA
    TACATGACCC CAAGTGCCAG CAGCCAGGGC AATCAAGTCA GTGAAAATGG AAAAAACCCT
    TCCTCGGGCA ATTCTTGGGT CCCTCTGAAC ACACTCCCAC CTCTGGTTCC TAAGGAGGCG
    GCCACGCTCT TTGTCACCCG TGATAACCCA GCAGGATGCA CTGGGCTACC CAGCTACTCT
    GAGCACCCCA CTCTACGAAG ACAGATACCA GAAAGACCTC CCAAGATTGG CCTTCTTGCC
    CGTGGTACCT CAAGGCTGGA AACAGGCCCA GGTGGGACCA ACAGGTTCCG GGAGCGGTCA
    CTGTCTGTAC CCACAGACTC GGGTGTTACC TCAGTGGACT ATGACGAAGA ACAGAAGACC
    AGTGAGACCC GCATCCTGCC TTATGCCAGT ACAAGCTCCG AGGGCAGTAA CAGTACTGAC
    AACATTGCAG CCCTCAGCAC TGAGCAGGAG GCACGGCACA GAAGGCAAAG ATCCAAAAGT
    ATTTCACTCA AGAAGGCCAA AAAGAAGCCC TCTCCACCCA TGCGAAGTGT CTCACTCGTC
    AAAGATGAGC CAGCCCTCCC ACCAGAAGGC GAATTGGTAC TGCCCAAGGA CCAGAGGCCT
    AGGAGCCTTT GCCTCTCCTT GGAACACCAA GGGCACCACC CACCTCACCC AGATGCTCAA
    GGTCACCCAG CTGTGCCAAT GCTCAAAGAT CCAGGAAGTA CGCAGTTCTC TCACCACTGG
    TATCTTACTG ACTGGAAGTC TGGTGACACC TACCAGTCCT TGTCCAGCTC CAGCACTGCC
    ACCGGCACCA CGGTCATTGA GTGCACTCAA GTTCAGGGAA GCTCAGAGTC TCTGGCCTCC
    CCTTCCACCT CCAGAGCGAC AACACCCTCC CAGCTCTCCA TTGAGGTGGA AGCCAGGGAA
    GTAGCCTCTC CTGGGAGGCC CACTGGACTG ATGTCCCCTT CCAGTGGTTA CTCCAGCCAG
    TCAGAGACAC CCACACCCAC TGTCTCCATG TCCTTGACCC TGGGCCACTT ACCTCCTCCA
    AGTGCTAGTG TCAGAGTACG TCCAGTGGTA CCAGAAAGGA AGTCATCACT GCCCCCAACA
    TCACCAATGG AGAAAATCTG CAAGTCACGG TTATCATTTG ACCTACCACT GACCTCTTCA
    ACCACCCTGG ATCTGTCGGG GATGAGTATC TCCATCCGCA GCAAAACCAA GGTGAGCCGC
    CATCACTCTG ATACCAATTT TGGGGTCAAA CTGGCACAGA AAACTAGTCC AAACCAACCG
    ATCATGCCCA TGGTTACTCA GTCTGACCTC CGTTCTGTTC GCCTGAGGTC AGTCAGCAAG
    TCTGAGCCAG AAGACGACAT TGAGAGTCCA GATTACATAG AGGAACCTGG AGCAGAGGAA
    GTCTTCACCA TGCCAGAGAG AAAAGTGAAA CCTCCCATAG CCGAGAAACC TCCATTGGCC
    CGAAGGCCTC CAAGTTTGGT CCACAGGCCG CCCTCTCTCC CTGGGGAGTA TCCACTAACT
    TCTCCTACTA TGGCTATGGC ATCCAGGAGC TCCATTCCAC ACATGAAGCA GCTTCCCCAA
    GACAGCTACA CAGTGTTGCG GAAACCAAAG TCACCCAGCT TCCCCGGGGA GTCAACAGCA
    TCCTCCAGCC TTGTCCTCTC ACCTCTTGCC AGTTCCTCTG GTGCTTTCTT CTCAGGAACA
    CAGCAACCTC CCCAGGCCAG TGTAGAAGAC GGGGGCCCCA AGGTGAGAGC CCTGCCTGAA
    AGAATCGGCC TCCAGAGCCA GGAAGAAGCT GAGAAAAAGA TGACCAAGAT TCCACCTCCG
    GTACCAAAAA AGCCCAGTGT GCTCTACCTG CCTCTCACCT CCCCTGTAGC TCAAATGGAT
    GCCTGCATGG CAGAACCAAG GCTACCTTTC AGCCCCATCA TCACCCTGGA GGAAGATGGC
    AAGTGTCCCT CCACTGGTGA TGACCAGAAG TCACCTGGTA AAGGGGTGAC TTCGCCTCTT
    CACACTGACA CAGAAAAAGA GGCCATCTCT CCAGGGAGGT CAGTGGAACC GAGTGCTGAA
    GAGAAAAGTT TAATCAGTGA TAAAACAGCC GAATGGATTG CAGAGGAGGA GGATGACGTG
    TTTGTGGCTT CCCGCACAAC TGAAGATTTA TTTACTGTGA TACACAGGTC CAAAAGAAAG
    CTACTGGGCT GGAAAGAGAC TGGGGAGGGC TTCACGGGAA GCAAACCCAG CTCCCATTCA
    CCAGTGAAGA ATACAGCTGA TTCTCCCACC GGTGAGGCTG CTGCTGCCCC AGGGCCAAGT
    AGCAGTGCCT GCCTAGATGC TGGTAGAAAT GATGATTTCA AGGCCTTGCT CCAGAAGAAG
    GGAAGTAAGG CAACTCCCAG GACCCGCCCC TCAGCGGCCG AGCTGCTGAA GACCACTAAC
    CCTCTGGCTC GAAGGATTAT TGCACAGTTC TCAAAAGACT ACGAACCCAC TGACAACCCC
    AGTACCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu75774
    Clone ID Related Accession (Same CDS sequence) XM_011247501.3 , XM_011247501.2
    Accession Version XM_011247501.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3252bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product NHS-like protein 2 isoform X8
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000086.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_011247501.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGCCGTTCT ACCGGCGCAC TGTGGTGCCC CAGCGTCTGT GCCCGCGCAA CCCTCCGCAG 
    CCGCTGGCTG AGCTCCGCGA CGTGAGCCAC CTGGCCGCGC TCAGTCTGCT CCGCCAGCTC
    GCAGACCTCT GCGGCCACTC GCTGGCTCTG CTGGAGGACC TCGAGGGGCA CCTGCTGGCT
    CTGGGGCGCC GCACCGACAG CCTGTACCGG CGCACCGTCC GGCTCCGCCG CCGCCTTCCC
    TGTCGCCTGC TTGGCCCGGA GGATGATGAA GAATTGCTAG GTGCCTGCCA CTGGGTGAAC
    GGGACTCGCA TCCTGAGGGC AAGGGAGCTT GCTGATGTAG CCAGCACAGG GCAGCAGTTT
    GATAGACATG CAAGTTTTCG ACACTCGTTG TTTAACACAG AGACAGCCGT GAACCCCAAG
    TCCACCCTGA GGCGGAGGCG GACCATTATT GGATTCTCTA ACTTTTCTCA GCGAGACCAA
    GGTCACAGCA GCAGCCCCAC AGGCAGCCTG GCCCGCTCTG CCACCTCAGA CATCAGGCCT
    GGCCACTCAG CTCCACAAGT TGTTCAGGGA AGAGTTGCTG TTGGGCAGGA AGCTCGGTTC
    CCAAGTCTCA CCTCACCAGG ACTGAGACAC TCTTCTAGTG AGCCCGGAGA CGCTCACCAG
    GCACGCAGTG CCTCAGACCA TCCCGGCATG GAGAGCATGG CAGTGGTGTA CAGTGTCCCC
    AGTTCTTGCA ATGGACCAAC AGAGTCAACA TTCTCCACTT CCTGGAAGGG AGATGCTTTC
    ACATACATGA CCCCAAGTGC CAGCAGCCAG GGCAATCAAG TCAGTGAAAA TGGAAAAAAC
    CCTTCCTCGG GCAATTCTTG GGTCCCTCTG AACACACTCC CACCTCTGGT TCCTAAGGAG
    GCGGCCACGC TCTTTGTCAC CCGTGATAAC CCAGCAGGAT GCACTGGGCT ACCCAGCTAC
    TCTGAGCACC CCACTCTACG AAGACAGATA CCAGAAAGAC CTCCCAAGAT TGGCCTTCTT
    GCCCGTGGTA CCTCAAGGCT GGAAACAGGC CCAGGTGGGA CCAACAGGTT CCGGGAGCGG
    TCACTGTCTG TACCCACAGA CTCGGGTGTT ACCTCAGTGG ACTATGACGA AGAACAGAAG
    ACCAGTGAGA CCCGCATCCT GCCTTATGCC AGTACAAGCT CCGAGGGCAG TAACAGTACT
    GACAACATTG CAGCCCTCAG CACTGAGCAG GAGGCACGGC ACAGAAGGCA AAGATCCAAA
    AGTATTTCAC TCAAGAAGGC CAAAAAGAAG CCCTCTCCAC CCATGCGAAG TGTCTCACTC
    GTCAAAGATG AGCCAGCCCT CCCACCAGAA GGCGAATTGG TACTGCCCAA GGACCAGAGG
    CCTAGGAGCC TTTGCCTCTC CTTGGAACAC CAAGGGCACC ACCCACCTCA CCCAGATGCT
    CAAGGTCACC CAGCTGTGCC AATGCTCAAA GATCCAGGAA GTACGCAGTT CTCTCACCAC
    TGGTATCTTA CTGACTGGAA GTCTGGTGAC ACCTACCAGT CCTTGTCCAG CTCCAGCACT
    GCCACCGGCA CCACGGTCAT TGAGTGCACT CAAGTTCAGG GAAGCTCAGA GTCTCTGGCC
    TCCCCTTCCA CCTCCAGAGC GACAACACCC TCCCAGCTCT CCATTGAGGT GGAAGCCAGG
    GAAGTAGCCT CTCCTGGGAG GCCCACTGGA CTGATGTCCC CTTCCAGTGG TTACTCCAGC
    CAGTCAGAGA CACCCACACC CACTGTCTCC ATGTCCTTGA CCCTGGGCCA CTTACCTCCT
    CCAAGTGCTA GTGTCAGAGT ACGTCCAGTG GTACCAGAAA GGAAGTCATC ACTGCCCCCA
    ACATCACCAA TGGAGAAAAT CTGCAAGTCA CGGTTATCAT TTGACCTACC ACTGACCTCT
    TCAACCACCC TGGATCTGTC GGGGATGAGT ATCTCCATCC GCAGCAAAAC CAAGGTGAGC
    CGCCATCACT CTGATACCAA TTTTGGGGTC AAACTGGCAC AGAAAACTAG TCCAAACCAA
    CCGATCATGC CCATGGTTAC TCAGTCTGAC CTCCGTTCTG TTCGCCTGAG GTCAGTCAGC
    AAGTCTGAGC CAGAAGACGA CATTGAGAGT CCAGATTACA TAGAGGAACC TGGAGCAGAG
    GAAGTCTTCA CCATGCCAGA GAGAAAAGTG AAACCTCCCA TAGCCGAGAA ACCTCCATTG
    GCCCGAAGGC CTCCAAGTTT GGTCCACAGG CCGCCCTCTC TCCCTGGGGA GTATCCACTA
    ACTTCTCCTA CTATGGCTAT GGCATCCAGG AGCTCCATTC CACACATGAA GCAGCTTCCC
    CAAGACAGCT ACACAGTGTT GCGGAAACCA AAGTCACCCA GCTTCCCCGG GGAGTCAACA
    GCATCCTCCA GCCTTGTCCT CTCACCTCTT GCCAGTTCCT CTGGTGCTTT CTTCTCAGGA
    ACACAGCAAC CTCCCCAGGC CAGTGTAGAA GACGGGGGCC CCAAGGTGAG AGCCCTGCCT
    GAAAGAATCG GCCTCCAGAG CCAGGAAGAA GCTGAGAAAA AGATGACCAA GATTCCACCT
    CCGGTACCAA AAAAGCCCAG TGTGCTCTAC CTGCCTCTCA CCTCCCCTGT AGCTCAAATG
    GATGCCTGCA TGGCAGAACC AAGGCTACCT TTCAGCCCCA TCATCACCCT GGAGGAAGAT
    GGCAAGTGTC CCTCCACTGG TGATGACCAG AAGTCACCTG GTAAAGGGGT GACTTCGCCT
    CTTCACACTG ACACAGAAAA AGAGGCCATC TCTCCAGGGA GGTCAGTGGA ACCGAGTGCT
    GAAGAGAAAA GTTTAATCAG TGATAAAACA GCCGAATGGA TTGCAGAGGA GGAGGATGAC
    GTGTTTGTGG CTTCCCGCAC AACTGAAGAT TTATTTACTG TGATACACAG GTCCAAAAGA
    AAGCTACTGG GCTGGAAAGA GACTGGGGAG GGCTTCACGG GAAGCAAACC CAGCTCCCAT
    TCACCAGTGA AGAATACAGC TGATTCTCCC ACCGGTGAGG CTGCTGCTGC CCCAGGGCCA
    AGTAGCAGTG CCTGCCTAGA TGCTGGTAGA AATGATGATT TCAAGGCCTT GCTCCAGAAG
    AAGGGAAGTA AGGCAACTCC CAGGACCCGC CCCTCAGCGG CCGAGCTGCT GAAGACCACT
    AACCCTCTGG CTCGAAGGAT TATTGCACAG TTCTCAAAAG ACTACGAACC CACTGACAAC
    CCCAGTACCT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011245803.1
    CDS308..3559
    Translation

    Target ORF information:

    RefSeq Version XM_011247501.3
    Organism Mus musculus(house mouse)
    Definition Mus musculus NHS-like 2 (Nhsl2), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011247501.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGCCGTTCT ACCGGCGCAC TGTGGTGCCC CAGCGTCTGT GCCCGCGCAA CCCTCCGCAG 
    CCGCTGGCTG AGCTCCGCGA CGTGAGCCAC CTGGCCGCGC TCAGTCTGCT CCGCCAGCTC
    GCAGACCTCT GCGGCCACTC GCTGGCTCTG CTGGAGGACC TCGAGGGGCA CCTGCTGGCT
    CTGGGGCGCC GCACCGACAG CCTGTACCGG CGCACCGTCC GGCTCCGCCG CCGCCTTCCC
    TGTCGCCTGC TTGGCCCGGA GGATGATGAA GAATTGCTAG GTGCCTGCCA CTGGGTGAAC
    GGGACTCGCA TCCTGAGGGC AAGGGAGCTT GCTGATGTAG CCAGCACAGG GCAGCAGTTT
    GATAGACATG CAAGTTTTCG ACACTCGTTG TTTAACACAG AGACAGCCGT GAACCCCAAG
    TCCACCCTGA GGCGGAGGCG GACCATTATT GGATTCTCTA ACTTTTCTCA GCGAGACCAA
    GGTCACAGCA GCAGCCCCAC AGGCAGCCTG GCCCGCTCTG CCACCTCAGA CATCAGGCCT
    GGCCACTCAG CTCCACAAGT TGTTCAGGGA AGAGTTGCTG TTGGGCAGGA AGCTCGGTTC
    CCAAGTCTCA CCTCACCAGG ACTGAGACAC TCTTCTAGTG AGCCCGGAGA CGCTCACCAG
    GCACGCAGTG CCTCAGACCA TCCCGGCATG GAGAGCATGG CAGTGGTGTA CAGTGTCCCC
    AGTTCTTGCA ATGGACCAAC AGAGTCAACA TTCTCCACTT CCTGGAAGGG AGATGCTTTC
    ACATACATGA CCCCAAGTGC CAGCAGCCAG GGCAATCAAG TCAGTGAAAA TGGAAAAAAC
    CCTTCCTCGG GCAATTCTTG GGTCCCTCTG AACACACTCC CACCTCTGGT TCCTAAGGAG
    GCGGCCACGC TCTTTGTCAC CCGTGATAAC CCAGCAGGAT GCACTGGGCT ACCCAGCTAC
    TCTGAGCACC CCACTCTACG AAGACAGATA CCAGAAAGAC CTCCCAAGAT TGGCCTTCTT
    GCCCGTGGTA CCTCAAGGCT GGAAACAGGC CCAGGTGGGA CCAACAGGTT CCGGGAGCGG
    TCACTGTCTG TACCCACAGA CTCGGGTGTT ACCTCAGTGG ACTATGACGA AGAACAGAAG
    ACCAGTGAGA CCCGCATCCT GCCTTATGCC AGTACAAGCT CCGAGGGCAG TAACAGTACT
    GACAACATTG CAGCCCTCAG CACTGAGCAG GAGGCACGGC ACAGAAGGCA AAGATCCAAA
    AGTATTTCAC TCAAGAAGGC CAAAAAGAAG CCCTCTCCAC CCATGCGAAG TGTCTCACTC
    GTCAAAGATG AGCCAGCCCT CCCACCAGAA GGCGAATTGG TACTGCCCAA GGACCAGAGG
    CCTAGGAGCC TTTGCCTCTC CTTGGAACAC CAAGGGCACC ACCCACCTCA CCCAGATGCT
    CAAGGTCACC CAGCTGTGCC AATGCTCAAA GATCCAGGAA GTACGCAGTT CTCTCACCAC
    TGGTATCTTA CTGACTGGAA GTCTGGTGAC ACCTACCAGT CCTTGTCCAG CTCCAGCACT
    GCCACCGGCA CCACGGTCAT TGAGTGCACT CAAGTTCAGG GAAGCTCAGA GTCTCTGGCC
    TCCCCTTCCA CCTCCAGAGC GACAACACCC TCCCAGCTCT CCATTGAGGT GGAAGCCAGG
    GAAGTAGCCT CTCCTGGGAG GCCCACTGGA CTGATGTCCC CTTCCAGTGG TTACTCCAGC
    CAGTCAGAGA CACCCACACC CACTGTCTCC ATGTCCTTGA CCCTGGGCCA CTTACCTCCT
    CCAAGTGCTA GTGTCAGAGT ACGTCCAGTG GTACCAGAAA GGAAGTCATC ACTGCCCCCA
    ACATCACCAA TGGAGAAAAT CTGCAAGTCA CGGTTATCAT TTGACCTACC ACTGACCTCT
    TCAACCACCC TGGATCTGTC GGGGATGAGT ATCTCCATCC GCAGCAAAAC CAAGGTGAGC
    CGCCATCACT CTGATACCAA TTTTGGGGTC AAACTGGCAC AGAAAACTAG TCCAAACCAA
    CCGATCATGC CCATGGTTAC TCAGTCTGAC CTCCGTTCTG TTCGCCTGAG GTCAGTCAGC
    AAGTCTGAGC CAGAAGACGA CATTGAGAGT CCAGATTACA TAGAGGAACC TGGAGCAGAG
    GAAGTCTTCA CCATGCCAGA GAGAAAAGTG AAACCTCCCA TAGCCGAGAA ACCTCCATTG
    GCCCGAAGGC CTCCAAGTTT GGTCCACAGG CCGCCCTCTC TCCCTGGGGA GTATCCACTA
    ACTTCTCCTA CTATGGCTAT GGCATCCAGG AGCTCCATTC CACACATGAA GCAGCTTCCC
    CAAGACAGCT ACACAGTGTT GCGGAAACCA AAGTCACCCA GCTTCCCCGG GGAGTCAACA
    GCATCCTCCA GCCTTGTCCT CTCACCTCTT GCCAGTTCCT CTGGTGCTTT CTTCTCAGGA
    ACACAGCAAC CTCCCCAGGC CAGTGTAGAA GACGGGGGCC CCAAGGTGAG AGCCCTGCCT
    GAAAGAATCG GCCTCCAGAG CCAGGAAGAA GCTGAGAAAA AGATGACCAA GATTCCACCT
    CCGGTACCAA AAAAGCCCAG TGTGCTCTAC CTGCCTCTCA CCTCCCCTGT AGCTCAAATG
    GATGCCTGCA TGGCAGAACC AAGGCTACCT TTCAGCCCCA TCATCACCCT GGAGGAAGAT
    GGCAAGTGTC CCTCCACTGG TGATGACCAG AAGTCACCTG GTAAAGGGGT GACTTCGCCT
    CTTCACACTG ACACAGAAAA AGAGGCCATC TCTCCAGGGA GGTCAGTGGA ACCGAGTGCT
    GAAGAGAAAA GTTTAATCAG TGATAAAACA GCCGAATGGA TTGCAGAGGA GGAGGATGAC
    GTGTTTGTGG CTTCCCGCAC AACTGAAGAT TTATTTACTG TGATACACAG GTCCAAAAGA
    AAGCTACTGG GCTGGAAAGA GACTGGGGAG GGCTTCACGG GAAGCAAACC CAGCTCCCAT
    TCACCAGTGA AGAATACAGC TGATTCTCCC ACCGGTGAGG CTGCTGCTGC CCCAGGGCCA
    AGTAGCAGTG CCTGCCTAGA TGCTGGTAGA AATGATGATT TCAAGGCCTT GCTCCAGAAG
    AAGGGAAGTA AGGCAACTCC CAGGACCCGC CCCTCAGCGG CCGAGCTGCT GAAGACCACT
    AACCCTCTGG CTCGAAGGAT TATTGCACAG TTCTCAAAAG ACTACGAACC CACTGACAAC
    CCCAGTACCT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu75775
    Clone ID Related Accession (Same CDS sequence) XM_011247502.2 , XM_011247502.3
    Accession Version XM_011247502.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3120bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product NHS-like protein 2 isoform X9
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000086.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_011247502.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    ATGCCGTTCT ACCGGCGCAC TGTGGTGCCC CAGCGTCTGT GCCCGCGCAA CCCTCCGCAG 
    CCGCTGGCTG AGCTCCGCGA CGTGAGCCAC CTGGCCGCGC TCAGTCTGCT CCGCCAGCTC
    GCAGACCTCT GCGGCCACTC GCTGGCTCTG CTGGAGGACC TCGAGGGGCA CCTGCTGGCT
    CTGGGGCGCC GCACCGACAG CCTGTACCGG CGCACCGTCC GGCTCCGCCG CCGCCTTCCC
    TGTCGCCTGC TTGGCCCGGA GGATGATGAA GAATTGCTAG GTGCCTGCCA CTGGGTGAAC
    GGGACTCGCA TCCTGAGGGC AAGGGAGCTT GCTGATGTAG CCAGCACAGG TCACAGCAGC
    AGCCCCACAG GCAGCCTGGC CCGCTCTGCC ACCTCAGACA TCAGGCCTGG CCACTCAGCT
    CCACAAGTTG TTCAGGGAAG AGTTGCTGTT GGGCAGGAAG CTCGGTTCCC AAGTCTCACC
    TCACCAGGAC TGAGACACTC TTCTAGTGAG CCCGGAGACG CTCACCAGGC ACGCAGTGCC
    TCAGACCATC CCGGCATGGA GAGCATGGCA GTGGTGTACA GTGTCCCCAG TTCTTGCAAT
    GGACCAACAG AGTCAACATT CTCCACTTCC TGGAAGGGAG ATGCTTTCAC ATACATGACC
    CCAAGTGCCA GCAGCCAGGG CAATCAAGTC AGTGAAAATG GAAAAAACCC TTCCTCGGGC
    AATTCTTGGG TCCCTCTGAA CACACTCCCA CCTCTGGTTC CTAAGGAGGC GGCCACGCTC
    TTTGTCACCC GTGATAACCC AGCAGGATGC ACTGGGCTAC CCAGCTACTC TGAGCACCCC
    ACTCTACGAA GACAGATACC AGAAAGACCT CCCAAGATTG GCCTTCTTGC CCGTGGTACC
    TCAAGGCTGG AAACAGGCCC AGGTGGGACC AACAGGTTCC GGGAGCGGTC ACTGTCTGTA
    CCCACAGACT CGGGTGTTAC CTCAGTGGAC TATGACGAAG AACAGAAGAC CAGTGAGACC
    CGCATCCTGC CTTATGCCAG TACAAGCTCC GAGGGCAGTA ACAGTACTGA CAACATTGCA
    GCCCTCAGCA CTGAGCAGGA GGCACGGCAC AGAAGGCAAA GATCCAAAAG TATTTCACTC
    AAGAAGGCCA AAAAGAAGCC CTCTCCACCC ATGCGAAGTG TCTCACTCGT CAAAGATGAG
    CCAGCCCTCC CACCAGAAGG CGAATTGGTA CTGCCCAAGG ACCAGAGGCC TAGGAGCCTT
    TGCCTCTCCT TGGAACACCA AGGGCACCAC CCACCTCACC CAGATGCTCA AGGTCACCCA
    GCTGTGCCAA TGCTCAAAGA TCCAGGAAGT ACGCAGTTCT CTCACCACTG GTATCTTACT
    GACTGGAAGT CTGGTGACAC CTACCAGTCC TTGTCCAGCT CCAGCACTGC CACCGGCACC
    ACGGTCATTG AGTGCACTCA AGTTCAGGGA AGCTCAGAGT CTCTGGCCTC CCCTTCCACC
    TCCAGAGCGA CAACACCCTC CCAGCTCTCC ATTGAGGTGG AAGCCAGGGA AGTAGCCTCT
    CCTGGGAGGC CCACTGGACT GATGTCCCCT TCCAGTGGTT ACTCCAGCCA GTCAGAGACA
    CCCACACCCA CTGTCTCCAT GTCCTTGACC CTGGGCCACT TACCTCCTCC AAGTGCTAGT
    GTCAGAGTAC GTCCAGTGGT ACCAGAAAGG AAGTCATCAC TGCCCCCAAC ATCACCAATG
    GAGAAAATCT GCAAGTCACG GTTATCATTT GACCTACCAC TGACCTCTTC AACCACCCTG
    GATCTGTCGG GGATGAGTAT CTCCATCCGC AGCAAAACCA AGGTGAGCCG CCATCACTCT
    GATACCAATT TTGGGGTCAA ACTGGCACAG AAAACTAGTC CAAACCAACC GATCATGCCC
    ATGGTTACTC AGTCTGACCT CCGTTCTGTT CGCCTGAGGT CAGTCAGCAA GTCTGAGCCA
    GAAGACGACA TTGAGAGTCC AGATTACATA GAGGAACCTG GAGCAGAGGA AGTCTTCACC
    ATGCCAGAGA GAAAAGTGAA ACCTCCCATA GCCGAGAAAC CTCCATTGGC CCGAAGGCCT
    CCAAGTTTGG TCCACAGGCC GCCCTCTCTC CCTGGGGAGT ATCCACTAAC TTCTCCTACT
    ATGGCTATGG CATCCAGGAG CTCCATTCCA CACATGAAGC AGCTTCCCCA AGACAGCTAC
    ACAGTGTTGC GGAAACCAAA GTCACCCAGC TTCCCCGGGG AGTCAACAGC ATCCTCCAGC
    CTTGTCCTCT CACCTCTTGC CAGTTCCTCT GGTGCTTTCT TCTCAGGAAC ACAGCAACCT
    CCCCAGGCCA GTGTAGAAGA CGGGGGCCCC AAGGTGAGAG CCCTGCCTGA AAGAATCGGC
    CTCCAGAGCC AGGAAGAAGC TGAGAAAAAG ATGACCAAGA TTCCACCTCC GGTACCAAAA
    AAGCCCAGTG TGCTCTACCT GCCTCTCACC TCCCCTGTAG CTCAAATGGA TGCCTGCATG
    GCAGAACCAA GGCTACCTTT CAGCCCCATC ATCACCCTGG AGGAAGATGG CAAGTGTCCC
    TCCACTGGTG ATGACCAGAA GTCACCTGGT AAAGGGGTGA CTTCGCCTCT TCACACTGAC
    ACAGAAAAAG AGGCCATCTC TCCAGGGAGG TCAGTGGAAC CGAGTGCTGA AGAGAAAAGT
    TTAATCAGTG ATAAAACAGC CGAATGGATT GCAGAGGAGG AGGATGACGT GTTTGTGGCT
    TCCCGCACAA CTGAAGATTT ATTTACTGTG ATACACAGGT CCAAAAGAAA GCTACTGGGC
    TGGAAAGAGA CTGGGGAGGG CTTCACGGGA AGCAAACCCA GCTCCCATTC ACCAGTGAAG
    AATACAGCTG ATTCTCCCAC CGGTGAGGCT GCTGCTGCCC CAGGGCCAAG TAGCAGTGCC
    TGCCTAGATG CTGGTAGAAA TGATGATTTC AAGGCCTTGC TCCAGAAGAA GGGAAGTAAG
    GCAACTCCCA GGACCCGCCC CTCAGCGGCC GAGCTGCTGA AGACCACTAA CCCTCTGGCT
    CGAAGGATTA TTGCACAGTT CTCAAAAGAC TACGAACCCA CTGACAACCC CAGTACCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011245804.1
    CDS308..3427
    Translation

    Target ORF information:

    RefSeq Version XM_011247502.3
    Organism Mus musculus(house mouse)
    Definition Mus musculus NHS-like 2 (Nhsl2), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011247502.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    ATGCCGTTCT ACCGGCGCAC TGTGGTGCCC CAGCGTCTGT GCCCGCGCAA CCCTCCGCAG 
    CCGCTGGCTG AGCTCCGCGA CGTGAGCCAC CTGGCCGCGC TCAGTCTGCT CCGCCAGCTC
    GCAGACCTCT GCGGCCACTC GCTGGCTCTG CTGGAGGACC TCGAGGGGCA CCTGCTGGCT
    CTGGGGCGCC GCACCGACAG CCTGTACCGG CGCACCGTCC GGCTCCGCCG CCGCCTTCCC
    TGTCGCCTGC TTGGCCCGGA GGATGATGAA GAATTGCTAG GTGCCTGCCA CTGGGTGAAC
    GGGACTCGCA TCCTGAGGGC AAGGGAGCTT GCTGATGTAG CCAGCACAGG TCACAGCAGC
    AGCCCCACAG GCAGCCTGGC CCGCTCTGCC ACCTCAGACA TCAGGCCTGG CCACTCAGCT
    CCACAAGTTG TTCAGGGAAG AGTTGCTGTT GGGCAGGAAG CTCGGTTCCC AAGTCTCACC
    TCACCAGGAC TGAGACACTC TTCTAGTGAG CCCGGAGACG CTCACCAGGC ACGCAGTGCC
    TCAGACCATC CCGGCATGGA GAGCATGGCA GTGGTGTACA GTGTCCCCAG TTCTTGCAAT
    GGACCAACAG AGTCAACATT CTCCACTTCC TGGAAGGGAG ATGCTTTCAC ATACATGACC
    CCAAGTGCCA GCAGCCAGGG CAATCAAGTC AGTGAAAATG GAAAAAACCC TTCCTCGGGC
    AATTCTTGGG TCCCTCTGAA CACACTCCCA CCTCTGGTTC CTAAGGAGGC GGCCACGCTC
    TTTGTCACCC GTGATAACCC AGCAGGATGC ACTGGGCTAC CCAGCTACTC TGAGCACCCC
    ACTCTACGAA GACAGATACC AGAAAGACCT CCCAAGATTG GCCTTCTTGC CCGTGGTACC
    TCAAGGCTGG AAACAGGCCC AGGTGGGACC AACAGGTTCC GGGAGCGGTC ACTGTCTGTA
    CCCACAGACT CGGGTGTTAC CTCAGTGGAC TATGACGAAG AACAGAAGAC CAGTGAGACC
    CGCATCCTGC CTTATGCCAG TACAAGCTCC GAGGGCAGTA ACAGTACTGA CAACATTGCA
    GCCCTCAGCA CTGAGCAGGA GGCACGGCAC AGAAGGCAAA GATCCAAAAG TATTTCACTC
    AAGAAGGCCA AAAAGAAGCC CTCTCCACCC ATGCGAAGTG TCTCACTCGT CAAAGATGAG
    CCAGCCCTCC CACCAGAAGG CGAATTGGTA CTGCCCAAGG ACCAGAGGCC TAGGAGCCTT
    TGCCTCTCCT TGGAACACCA AGGGCACCAC CCACCTCACC CAGATGCTCA AGGTCACCCA
    GCTGTGCCAA TGCTCAAAGA TCCAGGAAGT ACGCAGTTCT CTCACCACTG GTATCTTACT
    GACTGGAAGT CTGGTGACAC CTACCAGTCC TTGTCCAGCT CCAGCACTGC CACCGGCACC
    ACGGTCATTG AGTGCACTCA AGTTCAGGGA AGCTCAGAGT CTCTGGCCTC CCCTTCCACC
    TCCAGAGCGA CAACACCCTC CCAGCTCTCC ATTGAGGTGG AAGCCAGGGA AGTAGCCTCT
    CCTGGGAGGC CCACTGGACT GATGTCCCCT TCCAGTGGTT ACTCCAGCCA GTCAGAGACA
    CCCACACCCA CTGTCTCCAT GTCCTTGACC CTGGGCCACT TACCTCCTCC AAGTGCTAGT
    GTCAGAGTAC GTCCAGTGGT ACCAGAAAGG AAGTCATCAC TGCCCCCAAC ATCACCAATG
    GAGAAAATCT GCAAGTCACG GTTATCATTT GACCTACCAC TGACCTCTTC AACCACCCTG
    GATCTGTCGG GGATGAGTAT CTCCATCCGC AGCAAAACCA AGGTGAGCCG CCATCACTCT
    GATACCAATT TTGGGGTCAA ACTGGCACAG AAAACTAGTC CAAACCAACC GATCATGCCC
    ATGGTTACTC AGTCTGACCT CCGTTCTGTT CGCCTGAGGT CAGTCAGCAA GTCTGAGCCA
    GAAGACGACA TTGAGAGTCC AGATTACATA GAGGAACCTG GAGCAGAGGA AGTCTTCACC
    ATGCCAGAGA GAAAAGTGAA ACCTCCCATA GCCGAGAAAC CTCCATTGGC CCGAAGGCCT
    CCAAGTTTGG TCCACAGGCC GCCCTCTCTC CCTGGGGAGT ATCCACTAAC TTCTCCTACT
    ATGGCTATGG CATCCAGGAG CTCCATTCCA CACATGAAGC AGCTTCCCCA AGACAGCTAC
    ACAGTGTTGC GGAAACCAAA GTCACCCAGC TTCCCCGGGG AGTCAACAGC ATCCTCCAGC
    CTTGTCCTCT CACCTCTTGC CAGTTCCTCT GGTGCTTTCT TCTCAGGAAC ACAGCAACCT
    CCCCAGGCCA GTGTAGAAGA CGGGGGCCCC AAGGTGAGAG CCCTGCCTGA AAGAATCGGC
    CTCCAGAGCC AGGAAGAAGC TGAGAAAAAG ATGACCAAGA TTCCACCTCC GGTACCAAAA
    AAGCCCAGTG TGCTCTACCT GCCTCTCACC TCCCCTGTAG CTCAAATGGA TGCCTGCATG
    GCAGAACCAA GGCTACCTTT CAGCCCCATC ATCACCCTGG AGGAAGATGG CAAGTGTCCC
    TCCACTGGTG ATGACCAGAA GTCACCTGGT AAAGGGGTGA CTTCGCCTCT TCACACTGAC
    ACAGAAAAAG AGGCCATCTC TCCAGGGAGG TCAGTGGAAC CGAGTGCTGA AGAGAAAAGT
    TTAATCAGTG ATAAAACAGC CGAATGGATT GCAGAGGAGG AGGATGACGT GTTTGTGGCT
    TCCCGCACAA CTGAAGATTT ATTTACTGTG ATACACAGGT CCAAAAGAAA GCTACTGGGC
    TGGAAAGAGA CTGGGGAGGG CTTCACGGGA AGCAAACCCA GCTCCCATTC ACCAGTGAAG
    AATACAGCTG ATTCTCCCAC CGGTGAGGCT GCTGCTGCCC CAGGGCCAAG TAGCAGTGCC
    TGCCTAGATG CTGGTAGAAA TGATGATTTC AAGGCCTTGC TCCAGAAGAA GGGAAGTAAG
    GCAACTCCCA GGACCCGCCC CTCAGCGGCC GAGCTGCTGA AGACCACTAA CCCTCTGGCT
    CGAAGGATTA TTGCACAGTT CTCAAAAGAC TACGAACCCA CTGACAACCC CAGTACCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu83043
    Clone ID Related Accession (Same CDS sequence) XM_017318335.1
    Accession Version XM_017318335.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3408bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-21
    Organism Mus musculus(house mouse)
    Product NHS-like protein 2 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000086.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    ATGCCGTTCT ACCGGCGCAC TGTGGTGCCC CAGCGTCTGT GCCCGCGCAA CCCTCCGCAG 
    CCGCTGGCTG AGCTCCGCGA CGTGAGCCAC CTGGCCGCGC TCAGTCTGCT CCGCCAGCTC
    GCAGACCTCT GCGGCCACTC GCTGGCTCTG CTGGAGGACC TCGAGGGGCA CCTGCTGGCT
    CTGGGGCGCC GCACCGACAG CCTGTACCGG CGCACCGTCC GGCTCCGCCG CCGCCTTCCC
    TGTCGCCTGC TTGGCCCGGA GGATGATGAA GAATTGCTAG GTGCCTGCCA CTGGGTGAAC
    GGGACTCGCA TCCTGAGGGC AAGGGAGCTT GCTGATGTAG CCAGCACAGC TGCAGCTAAC
    TCGGGTCGGG AAAATGCGAC AGCGACTGCC CACTCGAGGT CGTCATGGCG ACAGCCAGTG
    AACGTGTTCC TCTCCTCGGG CAGGCCCCCG AGTGTAGAGG AACTGCTTCG CGAGGCGCAG
    CTCAATCTCC AGAGCCTGTT GCAAGGGCAG CAGTTTGATA GACATGCAAG TTTTCGACAC
    TCGTTGTTTA ACACAGAGAC AGCCGTGAAC CCCAAGTCCA CCCTGAGGCG GAGGCGGACC
    ATTATTGGAT TCTCTAACTT TTCTCAGCGA GACCAAGGTC ACAGCAGCAG CCCCACAGGC
    AGCCTGGCCC GCTCTGCCAC CTCAGACATC AGGCCTGGCC ACTCAGCTCC ACAAGTTGTT
    CAGGGAAGAG TTGCTGTTGG GCAGGAAGCT CGGTTCCCAA GTCTCACCTC ACCAGGACTG
    AGACACTCTT CTAGTGAGCC CGGAGACGCT CACCAGGCAC GCAGTGCCTC AGACCATCCC
    GGCATGGAGA GCATGGCAGT GGTGTACAGT GTCCCCAGTT CTTGCAATGG ACCAACAGAG
    TCAACATTCT CCACTTCCTG GAAGGGAGAT GCTTTCACAT ACATGACCCC AAGTGCCAGC
    AGCCAGGGCA ATCAAGTCAG TGAAAATGGA AAAAACCCTT CCTCGGGCAA TTCTTGGGTC
    CCTCTGAACA CACTCCCACC TCTGGTTCCT AAGGAGGCGG CCACGCTCTT TGTCACCCGT
    GATAACCCAG CAGGATGCAC TGGGCTACCC AGCTACTCTG AGCACCCCAC TCTACGAAGA
    CAGATACCAG AAAGACCTCC CAAGATTGGC CTTCTTGCCC GTGGTACCTC AAGGCTGGAA
    ACAGGCCCAG GTGGGACCAA CAGGTTCCGG GAGCGGTCAC TGTCTGTACC CACAGACTCG
    GGTGTTACCT CAGTGGACTA TGACGAAGAA CAGAAGACCA GTGAGACCCG CATCCTGCCT
    TATGCCAGTA CAAGCTCCGA GGGCAGTAAC AGTACTGACA ACATTGCAGC CCTCAGCACT
    GAGCAGGAGG CACGGCACAG AAGGCAAAGA TCCAAAAGTA TTTCACTCAA GAAGGCCAAA
    AAGAAGCCCT CTCCACCCAT GCGAAGTGTC TCACTCGTCA AAGATGAGCC AGCCCTCCCA
    CCAGAAGGCG AATTGGTACT GCCCAAGGAC CAGAGGCCTA GGAGCCTTTG CCTCTCCTTG
    GAACACCAAG GGCACCACCC ACCTCACCCA GATGCTCAAG GTCACCCAGC TGTGCCAATG
    CTCAAAGATC CAGGAAGTAC GCAGTTCTCT CACCACTGGT ATCTTACTGA CTGGAAGTCT
    GGTGACACCT ACCAGTCCTT GTCCAGCTCC AGCACTGCCA CCGGCACCAC GGTCATTGAG
    TGCACTCAAG TTCAGGGAAG CTCAGAGTCT CTGGCCTCCC CTTCCACCTC CAGAGCGACA
    ACACCCTCCC AGCTCTCCAT TGAGGTGGAA GCCAGGGAAG TAGCCTCTCC TGGGAGGCCC
    ACTGGACTGA TGTCCCCTTC CAGTGGTTAC TCCAGCCAGT CAGAGACACC CACACCCACT
    GTCTCCATGT CCTTGACCCT GGGCCACTTA CCTCCTCCAA GTGCTAGTGT CAGAGTACGT
    CCAGTGGTAC CAGAAAGGAA GTCATCACTG CCCCCAACAT CACCAATGGA GAAAATCTGC
    AAGTCACGGT TATCATTTGA CCTACCACTG ACCTCTTCAA CCACCCTGGA TCTGTCGGGG
    ATGAGTATCT CCATCCGCAG CAAAACCAAG GTGAGCCGCC ATCACTCTGA TACCAATTTT
    GGGGTCAAAC TGGCACAGAA AACTAGTCCA AACCAACCGA TCATGCCCAT GGTTACTCAG
    TCTGACCTCC GTTCTGTTCG CCTGAGGTCA GTCAGCAAGT CTGAGCCAGA AGACGACATT
    GAGAGTCCAG ATTACATAGA GGAACCTGGA GCAGAGGAAG TCTTCACCAT GCCAGAGAGA
    AAAGTGAAAC CTCCCATAGC CGAGAAACCT CCATTGGCCC GAAGGCCTCC AAGTTTGGTC
    CACAGGCCGC CCTCTCTCCC TGGGGAGTAT CCACTAACTT CTCCTACTAT GGCTATGGCA
    TCCAGGAGCT CCATTCCACA CATGAAGCAG CTTCCCCAAG ACAGCTACAC AGTGTTGCGG
    AAACCAAAGT CACCCAGCTT CCCCGGGGAG TCAACAGCAT CCTCCAGCCT TGTCCTCTCA
    CCTCTTGCCA GTTCCTCTGG TGCTTTCTTC TCAGGAACAC AGCAACCTCC CCAGGCCAGT
    GTAGAAGACG GGGGCCCCAA GGTGAGAGCC CTGCCTGAAA GAATCGGCCT CCAGAGCCAG
    GAAGAAGCTG AGAAAAAGAT GACCAAGATT CCACCTCCGG TACCAAAAAA GCCCAGTGTG
    CTCTACCTGC CTCTCACCTC CCCTGTAGCT CAAATGGATG CCTGCATGGC AGAACCAAGG
    CTACCTTTCA GCCCCATCAT CACCCTGGAG GAAGATGGCA AGTGTCCCTC CACTGGTGAT
    GACCAGAAGT CACCTGGTAA AGGGGTGACT TCGCCTCTTC ACACTGACAC AGAAAAAGAG
    GCCATCTCTC CAGGGAGGTC AGTGGAACCG AGTGCTGAAG AGAAAAGTTT AATCAGTGAT
    AAAACAGCCG AATGGATTGC AGAGGAGGAG GATGACGTGT TTGTGGCTTC CCGCACAACT
    GAAGATTTAT TTACTGTGAT ACACAGGTCC AAAAGAAAGC TACTGGGCTG GAAAGAGACT
    GGGGAGGGCT TCACGGGAAG CAAACCCAGC TCCCATTCAC CAGTGAAGAA TACAGCTGAT
    TCTCCCACCG GTGAGGCTGC TGCTGCCCCA GGGCCAAGTA GCAGTGCCTG CCTAGATGCT
    GGTAGAAATG ATGATTTCAA GGCCTTGCTC CAGAAGAAGG GAAGTAAGGC AACTCCCAGG
    ACCCGCCCCT CAGCGGCCGA GCTGCTGAAG ACCACTAACC CTCTGGCTCG AAGGATTATT
    GCACAGTTCT CAAAAGACTA CGAACCCACT GACAACCCCA GTACCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017173824.1
    CDS894..4301
    Translation

    Target ORF information:

    RefSeq Version XM_017318335.1
    Organism Mus musculus(house mouse)
    Definition Mus musculus NHS-like 2 (Nhsl2), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017318335.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    ATGCCGTTCT ACCGGCGCAC TGTGGTGCCC CAGCGTCTGT GCCCGCGCAA CCCTCCGCAG 
    CCGCTGGCTG AGCTCCGCGA CGTGAGCCAC CTGGCCGCGC TCAGTCTGCT CCGCCAGCTC
    GCAGACCTCT GCGGCCACTC GCTGGCTCTG CTGGAGGACC TCGAGGGGCA CCTGCTGGCT
    CTGGGGCGCC GCACCGACAG CCTGTACCGG CGCACCGTCC GGCTCCGCCG CCGCCTTCCC
    TGTCGCCTGC TTGGCCCGGA GGATGATGAA GAATTGCTAG GTGCCTGCCA CTGGGTGAAC
    GGGACTCGCA TCCTGAGGGC AAGGGAGCTT GCTGATGTAG CCAGCACAGC TGCAGCTAAC
    TCGGGTCGGG AAAATGCGAC AGCGACTGCC CACTCGAGGT CGTCATGGCG ACAGCCAGTG
    AACGTGTTCC TCTCCTCGGG CAGGCCCCCG AGTGTAGAGG AACTGCTTCG CGAGGCGCAG
    CTCAATCTCC AGAGCCTGTT GCAAGGGCAG CAGTTTGATA GACATGCAAG TTTTCGACAC
    TCGTTGTTTA ACACAGAGAC AGCCGTGAAC CCCAAGTCCA CCCTGAGGCG GAGGCGGACC
    ATTATTGGAT TCTCTAACTT TTCTCAGCGA GACCAAGGTC ACAGCAGCAG CCCCACAGGC
    AGCCTGGCCC GCTCTGCCAC CTCAGACATC AGGCCTGGCC ACTCAGCTCC ACAAGTTGTT
    CAGGGAAGAG TTGCTGTTGG GCAGGAAGCT CGGTTCCCAA GTCTCACCTC ACCAGGACTG
    AGACACTCTT CTAGTGAGCC CGGAGACGCT CACCAGGCAC GCAGTGCCTC AGACCATCCC
    GGCATGGAGA GCATGGCAGT GGTGTACAGT GTCCCCAGTT CTTGCAATGG ACCAACAGAG
    TCAACATTCT CCACTTCCTG GAAGGGAGAT GCTTTCACAT ACATGACCCC AAGTGCCAGC
    AGCCAGGGCA ATCAAGTCAG TGAAAATGGA AAAAACCCTT CCTCGGGCAA TTCTTGGGTC
    CCTCTGAACA CACTCCCACC TCTGGTTCCT AAGGAGGCGG CCACGCTCTT TGTCACCCGT
    GATAACCCAG CAGGATGCAC TGGGCTACCC AGCTACTCTG AGCACCCCAC TCTACGAAGA
    CAGATACCAG AAAGACCTCC CAAGATTGGC CTTCTTGCCC GTGGTACCTC AAGGCTGGAA
    ACAGGCCCAG GTGGGACCAA CAGGTTCCGG GAGCGGTCAC TGTCTGTACC CACAGACTCG
    GGTGTTACCT CAGTGGACTA TGACGAAGAA CAGAAGACCA GTGAGACCCG CATCCTGCCT
    TATGCCAGTA CAAGCTCCGA GGGCAGTAAC AGTACTGACA ACATTGCAGC CCTCAGCACT
    GAGCAGGAGG CACGGCACAG AAGGCAAAGA TCCAAAAGTA TTTCACTCAA GAAGGCCAAA
    AAGAAGCCCT CTCCACCCAT GCGAAGTGTC TCACTCGTCA AAGATGAGCC AGCCCTCCCA
    CCAGAAGGCG AATTGGTACT GCCCAAGGAC CAGAGGCCTA GGAGCCTTTG CCTCTCCTTG
    GAACACCAAG GGCACCACCC ACCTCACCCA GATGCTCAAG GTCACCCAGC TGTGCCAATG
    CTCAAAGATC CAGGAAGTAC GCAGTTCTCT CACCACTGGT ATCTTACTGA CTGGAAGTCT
    GGTGACACCT ACCAGTCCTT GTCCAGCTCC AGCACTGCCA CCGGCACCAC GGTCATTGAG
    TGCACTCAAG TTCAGGGAAG CTCAGAGTCT CTGGCCTCCC CTTCCACCTC CAGAGCGACA
    ACACCCTCCC AGCTCTCCAT TGAGGTGGAA GCCAGGGAAG TAGCCTCTCC TGGGAGGCCC
    ACTGGACTGA TGTCCCCTTC CAGTGGTTAC TCCAGCCAGT CAGAGACACC CACACCCACT
    GTCTCCATGT CCTTGACCCT GGGCCACTTA CCTCCTCCAA GTGCTAGTGT CAGAGTACGT
    CCAGTGGTAC CAGAAAGGAA GTCATCACTG CCCCCAACAT CACCAATGGA GAAAATCTGC
    AAGTCACGGT TATCATTTGA CCTACCACTG ACCTCTTCAA CCACCCTGGA TCTGTCGGGG
    ATGAGTATCT CCATCCGCAG CAAAACCAAG GTGAGCCGCC ATCACTCTGA TACCAATTTT
    GGGGTCAAAC TGGCACAGAA AACTAGTCCA AACCAACCGA TCATGCCCAT GGTTACTCAG
    TCTGACCTCC GTTCTGTTCG CCTGAGGTCA GTCAGCAAGT CTGAGCCAGA AGACGACATT
    GAGAGTCCAG ATTACATAGA GGAACCTGGA GCAGAGGAAG TCTTCACCAT GCCAGAGAGA
    AAAGTGAAAC CTCCCATAGC CGAGAAACCT CCATTGGCCC GAAGGCCTCC AAGTTTGGTC
    CACAGGCCGC CCTCTCTCCC TGGGGAGTAT CCACTAACTT CTCCTACTAT GGCTATGGCA
    TCCAGGAGCT CCATTCCACA CATGAAGCAG CTTCCCCAAG ACAGCTACAC AGTGTTGCGG
    AAACCAAAGT CACCCAGCTT CCCCGGGGAG TCAACAGCAT CCTCCAGCCT TGTCCTCTCA
    CCTCTTGCCA GTTCCTCTGG TGCTTTCTTC TCAGGAACAC AGCAACCTCC CCAGGCCAGT
    GTAGAAGACG GGGGCCCCAA GGTGAGAGCC CTGCCTGAAA GAATCGGCCT CCAGAGCCAG
    GAAGAAGCTG AGAAAAAGAT GACCAAGATT CCACCTCCGG TACCAAAAAA GCCCAGTGTG
    CTCTACCTGC CTCTCACCTC CCCTGTAGCT CAAATGGATG CCTGCATGGC AGAACCAAGG
    CTACCTTTCA GCCCCATCAT CACCCTGGAG GAAGATGGCA AGTGTCCCTC CACTGGTGAT
    GACCAGAAGT CACCTGGTAA AGGGGTGACT TCGCCTCTTC ACACTGACAC AGAAAAAGAG
    GCCATCTCTC CAGGGAGGTC AGTGGAACCG AGTGCTGAAG AGAAAAGTTT AATCAGTGAT
    AAAACAGCCG AATGGATTGC AGAGGAGGAG GATGACGTGT TTGTGGCTTC CCGCACAACT
    GAAGATTTAT TTACTGTGAT ACACAGGTCC AAAAGAAAGC TACTGGGCTG GAAAGAGACT
    GGGGAGGGCT TCACGGGAAG CAAACCCAGC TCCCATTCAC CAGTGAAGAA TACAGCTGAT
    TCTCCCACCG GTGAGGCTGC TGCTGCCCCA GGGCCAAGTA GCAGTGCCTG CCTAGATGCT
    GGTAGAAATG ATGATTTCAA GGCCTTGCTC CAGAAGAAGG GAAGTAAGGC AACTCCCAGG
    ACCCGCCCCT CAGCGGCCGA GCTGCTGAAG ACCACTAACC CTCTGGCTCG AAGGATTATT
    GCACAGTTCT CAAAAGACTA CGAACCCACT GACAACCCCA GTACCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu83044
    Clone ID Related Accession (Same CDS sequence) XM_017318336.1
    Accession Version XM_017318336.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3276bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-21
    Organism Mus musculus(house mouse)
    Product NHS-like protein 2 isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000086.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGCCGTTCT ACCGGCGCAC TGTGGTGCCC CAGCGTCTGT GCCCGCGCAA CCCTCCGCAG 
    CCGCTGGCTG AGCTCCGCGA CGTGAGCCAC CTGGCCGCGC TCAGTCTGCT CCGCCAGCTC
    GCAGACCTCT GCGGCCACTC GCTGGCTCTG CTGGAGGACC TCGAGGGGCA CCTGCTGGCT
    CTGGGGCGCC GCACCGACAG CCTGTACCGG CGCACCGTCC GGCTCCGCCG CCGCCTTCCC
    TGTCGCCTGC TTGGCCCGGA GGATGATGAA GAATTGCTAG GTGCCTGCCA CTGGGTGAAC
    GGGACTCGCA TCCTGAGGGC AAGGGAGCTT GCTGATGTAG CCAGCACAGC TGCAGCTAAC
    TCGGGTCGGG AAAATGCGAC AGCGACTGCC CACTCGAGGT CGTCATGGCG ACAGCCAGTG
    AACGTGTTCC TCTCCTCGGG CAGGCCCCCG AGTGTAGAGG AACTGCTTCG CGAGGCGCAG
    CTCAATCTCC AGAGCCTGTT GCAAGGTCAC AGCAGCAGCC CCACAGGCAG CCTGGCCCGC
    TCTGCCACCT CAGACATCAG GCCTGGCCAC TCAGCTCCAC AAGTTGTTCA GGGAAGAGTT
    GCTGTTGGGC AGGAAGCTCG GTTCCCAAGT CTCACCTCAC CAGGACTGAG ACACTCTTCT
    AGTGAGCCCG GAGACGCTCA CCAGGCACGC AGTGCCTCAG ACCATCCCGG CATGGAGAGC
    ATGGCAGTGG TGTACAGTGT CCCCAGTTCT TGCAATGGAC CAACAGAGTC AACATTCTCC
    ACTTCCTGGA AGGGAGATGC TTTCACATAC ATGACCCCAA GTGCCAGCAG CCAGGGCAAT
    CAAGTCAGTG AAAATGGAAA AAACCCTTCC TCGGGCAATT CTTGGGTCCC TCTGAACACA
    CTCCCACCTC TGGTTCCTAA GGAGGCGGCC ACGCTCTTTG TCACCCGTGA TAACCCAGCA
    GGATGCACTG GGCTACCCAG CTACTCTGAG CACCCCACTC TACGAAGACA GATACCAGAA
    AGACCTCCCA AGATTGGCCT TCTTGCCCGT GGTACCTCAA GGCTGGAAAC AGGCCCAGGT
    GGGACCAACA GGTTCCGGGA GCGGTCACTG TCTGTACCCA CAGACTCGGG TGTTACCTCA
    GTGGACTATG ACGAAGAACA GAAGACCAGT GAGACCCGCA TCCTGCCTTA TGCCAGTACA
    AGCTCCGAGG GCAGTAACAG TACTGACAAC ATTGCAGCCC TCAGCACTGA GCAGGAGGCA
    CGGCACAGAA GGCAAAGATC CAAAAGTATT TCACTCAAGA AGGCCAAAAA GAAGCCCTCT
    CCACCCATGC GAAGTGTCTC ACTCGTCAAA GATGAGCCAG CCCTCCCACC AGAAGGCGAA
    TTGGTACTGC CCAAGGACCA GAGGCCTAGG AGCCTTTGCC TCTCCTTGGA ACACCAAGGG
    CACCACCCAC CTCACCCAGA TGCTCAAGGT CACCCAGCTG TGCCAATGCT CAAAGATCCA
    GGAAGTACGC AGTTCTCTCA CCACTGGTAT CTTACTGACT GGAAGTCTGG TGACACCTAC
    CAGTCCTTGT CCAGCTCCAG CACTGCCACC GGCACCACGG TCATTGAGTG CACTCAAGTT
    CAGGGAAGCT CAGAGTCTCT GGCCTCCCCT TCCACCTCCA GAGCGACAAC ACCCTCCCAG
    CTCTCCATTG AGGTGGAAGC CAGGGAAGTA GCCTCTCCTG GGAGGCCCAC TGGACTGATG
    TCCCCTTCCA GTGGTTACTC CAGCCAGTCA GAGACACCCA CACCCACTGT CTCCATGTCC
    TTGACCCTGG GCCACTTACC TCCTCCAAGT GCTAGTGTCA GAGTACGTCC AGTGGTACCA
    GAAAGGAAGT CATCACTGCC CCCAACATCA CCAATGGAGA AAATCTGCAA GTCACGGTTA
    TCATTTGACC TACCACTGAC CTCTTCAACC ACCCTGGATC TGTCGGGGAT GAGTATCTCC
    ATCCGCAGCA AAACCAAGGT GAGCCGCCAT CACTCTGATA CCAATTTTGG GGTCAAACTG
    GCACAGAAAA CTAGTCCAAA CCAACCGATC ATGCCCATGG TTACTCAGTC TGACCTCCGT
    TCTGTTCGCC TGAGGTCAGT CAGCAAGTCT GAGCCAGAAG ACGACATTGA GAGTCCAGAT
    TACATAGAGG AACCTGGAGC AGAGGAAGTC TTCACCATGC CAGAGAGAAA AGTGAAACCT
    CCCATAGCCG AGAAACCTCC ATTGGCCCGA AGGCCTCCAA GTTTGGTCCA CAGGCCGCCC
    TCTCTCCCTG GGGAGTATCC ACTAACTTCT CCTACTATGG CTATGGCATC CAGGAGCTCC
    ATTCCACACA TGAAGCAGCT TCCCCAAGAC AGCTACACAG TGTTGCGGAA ACCAAAGTCA
    CCCAGCTTCC CCGGGGAGTC AACAGCATCC TCCAGCCTTG TCCTCTCACC TCTTGCCAGT
    TCCTCTGGTG CTTTCTTCTC AGGAACACAG CAACCTCCCC AGGCCAGTGT AGAAGACGGG
    GGCCCCAAGG TGAGAGCCCT GCCTGAAAGA ATCGGCCTCC AGAGCCAGGA AGAAGCTGAG
    AAAAAGATGA CCAAGATTCC ACCTCCGGTA CCAAAAAAGC CCAGTGTGCT CTACCTGCCT
    CTCACCTCCC CTGTAGCTCA AATGGATGCC TGCATGGCAG AACCAAGGCT ACCTTTCAGC
    CCCATCATCA CCCTGGAGGA AGATGGCAAG TGTCCCTCCA CTGGTGATGA CCAGAAGTCA
    CCTGGTAAAG GGGTGACTTC GCCTCTTCAC ACTGACACAG AAAAAGAGGC CATCTCTCCA
    GGGAGGTCAG TGGAACCGAG TGCTGAAGAG AAAAGTTTAA TCAGTGATAA AACAGCCGAA
    TGGATTGCAG AGGAGGAGGA TGACGTGTTT GTGGCTTCCC GCACAACTGA AGATTTATTT
    ACTGTGATAC ACAGGTCCAA AAGAAAGCTA CTGGGCTGGA AAGAGACTGG GGAGGGCTTC
    ACGGGAAGCA AACCCAGCTC CCATTCACCA GTGAAGAATA CAGCTGATTC TCCCACCGGT
    GAGGCTGCTG CTGCCCCAGG GCCAAGTAGC AGTGCCTGCC TAGATGCTGG TAGAAATGAT
    GATTTCAAGG CCTTGCTCCA GAAGAAGGGA AGTAAGGCAA CTCCCAGGAC CCGCCCCTCA
    GCGGCCGAGC TGCTGAAGAC CACTAACCCT CTGGCTCGAA GGATTATTGC ACAGTTCTCA
    AAAGACTACG AACCCACTGA CAACCCCAGT ACCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017173825.1
    CDS893..4168
    Translation

    Target ORF information:

    RefSeq Version XM_017318336.1
    Organism Mus musculus(house mouse)
    Definition Mus musculus NHS-like 2 (Nhsl2), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017318336.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGCCGTTCT ACCGGCGCAC TGTGGTGCCC CAGCGTCTGT GCCCGCGCAA CCCTCCGCAG 
    CCGCTGGCTG AGCTCCGCGA CGTGAGCCAC CTGGCCGCGC TCAGTCTGCT CCGCCAGCTC
    GCAGACCTCT GCGGCCACTC GCTGGCTCTG CTGGAGGACC TCGAGGGGCA CCTGCTGGCT
    CTGGGGCGCC GCACCGACAG CCTGTACCGG CGCACCGTCC GGCTCCGCCG CCGCCTTCCC
    TGTCGCCTGC TTGGCCCGGA GGATGATGAA GAATTGCTAG GTGCCTGCCA CTGGGTGAAC
    GGGACTCGCA TCCTGAGGGC AAGGGAGCTT GCTGATGTAG CCAGCACAGC TGCAGCTAAC
    TCGGGTCGGG AAAATGCGAC AGCGACTGCC CACTCGAGGT CGTCATGGCG ACAGCCAGTG
    AACGTGTTCC TCTCCTCGGG CAGGCCCCCG AGTGTAGAGG AACTGCTTCG CGAGGCGCAG
    CTCAATCTCC AGAGCCTGTT GCAAGGTCAC AGCAGCAGCC CCACAGGCAG CCTGGCCCGC
    TCTGCCACCT CAGACATCAG GCCTGGCCAC TCAGCTCCAC AAGTTGTTCA GGGAAGAGTT
    GCTGTTGGGC AGGAAGCTCG GTTCCCAAGT CTCACCTCAC CAGGACTGAG ACACTCTTCT
    AGTGAGCCCG GAGACGCTCA CCAGGCACGC AGTGCCTCAG ACCATCCCGG CATGGAGAGC
    ATGGCAGTGG TGTACAGTGT CCCCAGTTCT TGCAATGGAC CAACAGAGTC AACATTCTCC
    ACTTCCTGGA AGGGAGATGC TTTCACATAC ATGACCCCAA GTGCCAGCAG CCAGGGCAAT
    CAAGTCAGTG AAAATGGAAA AAACCCTTCC TCGGGCAATT CTTGGGTCCC TCTGAACACA
    CTCCCACCTC TGGTTCCTAA GGAGGCGGCC ACGCTCTTTG TCACCCGTGA TAACCCAGCA
    GGATGCACTG GGCTACCCAG CTACTCTGAG CACCCCACTC TACGAAGACA GATACCAGAA
    AGACCTCCCA AGATTGGCCT TCTTGCCCGT GGTACCTCAA GGCTGGAAAC AGGCCCAGGT
    GGGACCAACA GGTTCCGGGA GCGGTCACTG TCTGTACCCA CAGACTCGGG TGTTACCTCA
    GTGGACTATG ACGAAGAACA GAAGACCAGT GAGACCCGCA TCCTGCCTTA TGCCAGTACA
    AGCTCCGAGG GCAGTAACAG TACTGACAAC ATTGCAGCCC TCAGCACTGA GCAGGAGGCA
    CGGCACAGAA GGCAAAGATC CAAAAGTATT TCACTCAAGA AGGCCAAAAA GAAGCCCTCT
    CCACCCATGC GAAGTGTCTC ACTCGTCAAA GATGAGCCAG CCCTCCCACC AGAAGGCGAA
    TTGGTACTGC CCAAGGACCA GAGGCCTAGG AGCCTTTGCC TCTCCTTGGA ACACCAAGGG
    CACCACCCAC CTCACCCAGA TGCTCAAGGT CACCCAGCTG TGCCAATGCT CAAAGATCCA
    GGAAGTACGC AGTTCTCTCA CCACTGGTAT CTTACTGACT GGAAGTCTGG TGACACCTAC
    CAGTCCTTGT CCAGCTCCAG CACTGCCACC GGCACCACGG TCATTGAGTG CACTCAAGTT
    CAGGGAAGCT CAGAGTCTCT GGCCTCCCCT TCCACCTCCA GAGCGACAAC ACCCTCCCAG
    CTCTCCATTG AGGTGGAAGC CAGGGAAGTA GCCTCTCCTG GGAGGCCCAC TGGACTGATG
    TCCCCTTCCA GTGGTTACTC CAGCCAGTCA GAGACACCCA CACCCACTGT CTCCATGTCC
    TTGACCCTGG GCCACTTACC TCCTCCAAGT GCTAGTGTCA GAGTACGTCC AGTGGTACCA
    GAAAGGAAGT CATCACTGCC CCCAACATCA CCAATGGAGA AAATCTGCAA GTCACGGTTA
    TCATTTGACC TACCACTGAC CTCTTCAACC ACCCTGGATC TGTCGGGGAT GAGTATCTCC
    ATCCGCAGCA AAACCAAGGT GAGCCGCCAT CACTCTGATA CCAATTTTGG GGTCAAACTG
    GCACAGAAAA CTAGTCCAAA CCAACCGATC ATGCCCATGG TTACTCAGTC TGACCTCCGT
    TCTGTTCGCC TGAGGTCAGT CAGCAAGTCT GAGCCAGAAG ACGACATTGA GAGTCCAGAT
    TACATAGAGG AACCTGGAGC AGAGGAAGTC TTCACCATGC CAGAGAGAAA AGTGAAACCT
    CCCATAGCCG AGAAACCTCC ATTGGCCCGA AGGCCTCCAA GTTTGGTCCA CAGGCCGCCC
    TCTCTCCCTG GGGAGTATCC ACTAACTTCT CCTACTATGG CTATGGCATC CAGGAGCTCC
    ATTCCACACA TGAAGCAGCT TCCCCAAGAC AGCTACACAG TGTTGCGGAA ACCAAAGTCA
    CCCAGCTTCC CCGGGGAGTC AACAGCATCC TCCAGCCTTG TCCTCTCACC TCTTGCCAGT
    TCCTCTGGTG CTTTCTTCTC AGGAACACAG CAACCTCCCC AGGCCAGTGT AGAAGACGGG
    GGCCCCAAGG TGAGAGCCCT GCCTGAAAGA ATCGGCCTCC AGAGCCAGGA AGAAGCTGAG
    AAAAAGATGA CCAAGATTCC ACCTCCGGTA CCAAAAAAGC CCAGTGTGCT CTACCTGCCT
    CTCACCTCCC CTGTAGCTCA AATGGATGCC TGCATGGCAG AACCAAGGCT ACCTTTCAGC
    CCCATCATCA CCCTGGAGGA AGATGGCAAG TGTCCCTCCA CTGGTGATGA CCAGAAGTCA
    CCTGGTAAAG GGGTGACTTC GCCTCTTCAC ACTGACACAG AAAAAGAGGC CATCTCTCCA
    GGGAGGTCAG TGGAACCGAG TGCTGAAGAG AAAAGTTTAA TCAGTGATAA AACAGCCGAA
    TGGATTGCAG AGGAGGAGGA TGACGTGTTT GTGGCTTCCC GCACAACTGA AGATTTATTT
    ACTGTGATAC ACAGGTCCAA AAGAAAGCTA CTGGGCTGGA AAGAGACTGG GGAGGGCTTC
    ACGGGAAGCA AACCCAGCTC CCATTCACCA GTGAAGAATA CAGCTGATTC TCCCACCGGT
    GAGGCTGCTG CTGCCCCAGG GCCAAGTAGC AGTGCCTGCC TAGATGCTGG TAGAAATGAT
    GATTTCAAGG CCTTGCTCCA GAAGAAGGGA AGTAAGGCAA CTCCCAGGAC CCGCCCCTCA
    GCGGCCGAGC TGCTGAAGAC CACTAACCCT CTGGCTCGAA GGATTATTGC ACAGTTCTCA
    AAAGACTACG AACCCACTGA CAACCCCAGT ACCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu90352
    Clone ID Related Accession (Same CDS sequence) XM_030251164.1
    Accession Version XM_030251164.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3102bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product NHS-like protein 2 isoform X10
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000086.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    ATGGAGAGAG GCGAGTCTCT TACCCTCTTC TGGAGCCGGG GAGCTGCAGC TAACTCGGGT 
    CGGGAAAATG CGACAGCGAC TGCCCACTCG AGGTCGTCAT GGCGACAGCC AGTGAACGTG
    TTCCTCTCCT CGGGCAGGCC CCCGAGTGTA GAGGAACTGC TTCGCGAGGC GCAGCTCAAT
    CTCCAGAGCC TGTTGCAAGG GCAGCAGTTT GATAGACATG CAAGTTTTCG ACACTCGTTG
    TTTAACACAG AGACAGCCGT GAACCCCAAG TCCACCCTGA GGCGGAGGCG GACCATTATT
    GGATTCTCTA ACTTTTCTCA GCGAGACCAA GGTCACAGCA GCAGCCCCAC AGGCAGCCTG
    GCCCGCTCTG CCACCTCAGA CATCAGGCCT GGCCACTCAG CTCCACAAGT TGTTCAGGGA
    AGAGTTGCTG TTGGGCAGGA AGCTCGGTTC CCAAGTCTCA CCTCACCAGG ACTGAGACAC
    TCTTCTAGTG AGCCCGGAGA CGCTCACCAG GCACGCAGTG CCTCAGACCA TCCCGGCATG
    GAGAGCATGG CAGTGGTGTA CAGTGTCCCC AGTTCTTGCA ATGGACCAAC AGAGTCAACA
    TTCTCCACTT CCTGGAAGGG AGATGCTTTC ACATACATGA CCCCAAGTGC CAGCAGCCAG
    GGCAATCAAG TCAGTGAAAA TGGAAAAAAC CCTTCCTCGG GCAATTCTTG GGTCCCTCTG
    AACACACTCC CACCTCTGGT TCCTAAGGAG GCGGCCACGC TCTTTGTCAC CCGTGATAAC
    CCAGCAGGAT GCACTGGGCT ACCCAGCTAC TCTGAGCACC CCACTCTACG AAGACAGATA
    CCAGAAAGAC CTCCCAAGAT TGGCCTTCTT GCCCGTGGTA CCTCAAGGCT GGAAACAGGC
    CCAGGTGGGA CCAACAGGTT CCGGGAGCGG TCACTGTCTG TACCCACAGA CTCGGGTGTT
    ACCTCAGTGG ACTATGACGA AGAACAGAAG ACCAGTGAGA CCCGCATCCT GCCTTATGCC
    AGTACAAGCT CCGAGGGCAG TAACAGTACT GACAACATTG CAGCCCTCAG CACTGAGCAG
    GAGGCACGGC ACAGAAGGCA AAGATCCAAA AGTATTTCAC TCAAGAAGGC CAAAAAGAAG
    CCCTCTCCAC CCATGCGAAG TGTCTCACTC GTCAAAGATG AGCCAGCCCT CCCACCAGAA
    GGCGAATTGG TACTGCCCAA GGACCAGAGG CCTAGGAGCC TTTGCCTCTC CTTGGAACAC
    CAAGGGCACC ACCCACCTCA CCCAGATGCT CAAGGTCACC CAGCTGTGCC AATGCTCAAA
    GATCCAGGAA GTACGCAGTT CTCTCACCAC TGGTATCTTA CTGACTGGAA GTCTGGTGAC
    ACCTACCAGT CCTTGTCCAG CTCCAGCACT GCCACCGGCA CCACGGTCAT TGAGTGCACT
    CAAGTTCAGG GAAGCTCAGA GTCTCTGGCC TCCCCTTCCA CCTCCAGAGC GACAACACCC
    TCCCAGCTCT CCATTGAGGT GGAAGCCAGG GAAGTAGCCT CTCCTGGGAG GCCCACTGGA
    CTGATGTCCC CTTCCAGTGG TTACTCCAGC CAGTCAGAGA CACCCACACC CACTGTCTCC
    ATGTCCTTGA CCCTGGGCCA CTTACCTCCT CCAAGTGCTA GTGTCAGAGT ACGTCCAGTG
    GTACCAGAAA GGAAGTCATC ACTGCCCCCA ACATCACCAA TGGAGAAAAT CTGCAAGTCA
    CGGTTATCAT TTGACCTACC ACTGACCTCT TCAACCACCC TGGATCTGTC GGGGATGAGT
    ATCTCCATCC GCAGCAAAAC CAAGGTGAGC CGCCATCACT CTGATACCAA TTTTGGGGTC
    AAACTGGCAC AGAAAACTAG TCCAAACCAA CCGATCATGC CCATGGTTAC TCAGTCTGAC
    CTCCGTTCTG TTCGCCTGAG GTCAGTCAGC AAGTCTGAGC CAGAAGACGA CATTGAGAGT
    CCAGATTACA TAGAGGAACC TGGAGCAGAG GAAGTCTTCA CCATGCCAGA GAGAAAAGTG
    AAACCTCCCA TAGCCGAGAA ACCTCCATTG GCCCGAAGGC CTCCAAGTTT GGTCCACAGG
    CCGCCCTCTC TCCCTGGGGA GTATCCACTA ACTTCTCCTA CTATGGCTAT GGCATCCAGG
    AGCTCCATTC CACACATGAA GCAGCTTCCC CAAGACAGCT ACACAGTGTT GCGGAAACCA
    AAGTCACCCA GCTTCCCCGG GGAGTCAACA GCATCCTCCA GCCTTGTCCT CTCACCTCTT
    GCCAGTTCCT CTGGTGCTTT CTTCTCAGGA ACACAGCAAC CTCCCCAGGC CAGTGTAGAA
    GACGGGGGCC CCAAGGTGAG AGCCCTGCCT GAAAGAATCG GCCTCCAGAG CCAGGAAGAA
    GCTGAGAAAA AGATGACCAA GATTCCACCT CCGGTACCAA AAAAGCCCAG TGTGCTCTAC
    CTGCCTCTCA CCTCCCCTGT AGCTCAAATG GATGCCTGCA TGGCAGAACC AAGGCTACCT
    TTCAGCCCCA TCATCACCCT GGAGGAAGAT GGCAAGTGTC CCTCCACTGG TGATGACCAG
    AAGTCACCTG GTAAAGGGGT GACTTCGCCT CTTCACACTG ACACAGAAAA AGAGGCCATC
    TCTCCAGGGA GGTCAGTGGA ACCGAGTGCT GAAGAGAAAA GTTTAATCAG TGATAAAACA
    GCCGAATGGA TTGCAGAGGA GGAGGATGAC GTGTTTGTGG CTTCCCGCAC AACTGAAGAT
    TTATTTACTG TGATACACAG GTCCAAAAGA AAGCTACTGG GCTGGAAAGA GACTGGGGAG
    GGCTTCACGG GAAGCAAACC CAGCTCCCAT TCACCAGTGA AGAATACAGC TGATTCTCCC
    ACCGGTGAGG CTGCTGCTGC CCCAGGGCCA AGTAGCAGTG CCTGCCTAGA TGCTGGTAGA
    AATGATGATT TCAAGGCCTT GCTCCAGAAG AAGGGAAGTA AGGCAACTCC CAGGACCCGC
    CCCTCAGCGG CCGAGCTGCT GAAGACCACT AACCCTCTGG CTCGAAGGAT TATTGCACAG
    TTCTCAAAAG ACTACGAACC CACTGACAAC CCCAGTACCT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_030107024.1
    CDS5961..9062
    Translation

    Target ORF information:

    RefSeq Version XM_030251164.1
    Organism Mus musculus(house mouse)
    Definition Mus musculus NHS-like 2 (Nhsl2), transcript variant X10, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_030251164.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    ATGGAGAGAG GCGAGTCTCT TACCCTCTTC TGGAGCCGGG GAGCTGCAGC TAACTCGGGT 
    CGGGAAAATG CGACAGCGAC TGCCCACTCG AGGTCGTCAT GGCGACAGCC AGTGAACGTG
    TTCCTCTCCT CGGGCAGGCC CCCGAGTGTA GAGGAACTGC TTCGCGAGGC GCAGCTCAAT
    CTCCAGAGCC TGTTGCAAGG GCAGCAGTTT GATAGACATG CAAGTTTTCG ACACTCGTTG
    TTTAACACAG AGACAGCCGT GAACCCCAAG TCCACCCTGA GGCGGAGGCG GACCATTATT
    GGATTCTCTA ACTTTTCTCA GCGAGACCAA GGTCACAGCA GCAGCCCCAC AGGCAGCCTG
    GCCCGCTCTG CCACCTCAGA CATCAGGCCT GGCCACTCAG CTCCACAAGT TGTTCAGGGA
    AGAGTTGCTG TTGGGCAGGA AGCTCGGTTC CCAAGTCTCA CCTCACCAGG ACTGAGACAC
    TCTTCTAGTG AGCCCGGAGA CGCTCACCAG GCACGCAGTG CCTCAGACCA TCCCGGCATG
    GAGAGCATGG CAGTGGTGTA CAGTGTCCCC AGTTCTTGCA ATGGACCAAC AGAGTCAACA
    TTCTCCACTT CCTGGAAGGG AGATGCTTTC ACATACATGA CCCCAAGTGC CAGCAGCCAG
    GGCAATCAAG TCAGTGAAAA TGGAAAAAAC CCTTCCTCGG GCAATTCTTG GGTCCCTCTG
    AACACACTCC CACCTCTGGT TCCTAAGGAG GCGGCCACGC TCTTTGTCAC CCGTGATAAC
    CCAGCAGGAT GCACTGGGCT ACCCAGCTAC TCTGAGCACC CCACTCTACG AAGACAGATA
    CCAGAAAGAC CTCCCAAGAT TGGCCTTCTT GCCCGTGGTA CCTCAAGGCT GGAAACAGGC
    CCAGGTGGGA CCAACAGGTT CCGGGAGCGG TCACTGTCTG TACCCACAGA CTCGGGTGTT
    ACCTCAGTGG ACTATGACGA AGAACAGAAG ACCAGTGAGA CCCGCATCCT GCCTTATGCC
    AGTACAAGCT CCGAGGGCAG TAACAGTACT GACAACATTG CAGCCCTCAG CACTGAGCAG
    GAGGCACGGC ACAGAAGGCA AAGATCCAAA AGTATTTCAC TCAAGAAGGC CAAAAAGAAG
    CCCTCTCCAC CCATGCGAAG TGTCTCACTC GTCAAAGATG AGCCAGCCCT CCCACCAGAA
    GGCGAATTGG TACTGCCCAA GGACCAGAGG CCTAGGAGCC TTTGCCTCTC CTTGGAACAC
    CAAGGGCACC ACCCACCTCA CCCAGATGCT CAAGGTCACC CAGCTGTGCC AATGCTCAAA
    GATCCAGGAA GTACGCAGTT CTCTCACCAC TGGTATCTTA CTGACTGGAA GTCTGGTGAC
    ACCTACCAGT CCTTGTCCAG CTCCAGCACT GCCACCGGCA CCACGGTCAT TGAGTGCACT
    CAAGTTCAGG GAAGCTCAGA GTCTCTGGCC TCCCCTTCCA CCTCCAGAGC GACAACACCC
    TCCCAGCTCT CCATTGAGGT GGAAGCCAGG GAAGTAGCCT CTCCTGGGAG GCCCACTGGA
    CTGATGTCCC CTTCCAGTGG TTACTCCAGC CAGTCAGAGA CACCCACACC CACTGTCTCC
    ATGTCCTTGA CCCTGGGCCA CTTACCTCCT CCAAGTGCTA GTGTCAGAGT ACGTCCAGTG
    GTACCAGAAA GGAAGTCATC ACTGCCCCCA ACATCACCAA TGGAGAAAAT CTGCAAGTCA
    CGGTTATCAT TTGACCTACC ACTGACCTCT TCAACCACCC TGGATCTGTC GGGGATGAGT
    ATCTCCATCC GCAGCAAAAC CAAGGTGAGC CGCCATCACT CTGATACCAA TTTTGGGGTC
    AAACTGGCAC AGAAAACTAG TCCAAACCAA CCGATCATGC CCATGGTTAC TCAGTCTGAC
    CTCCGTTCTG TTCGCCTGAG GTCAGTCAGC AAGTCTGAGC CAGAAGACGA CATTGAGAGT
    CCAGATTACA TAGAGGAACC TGGAGCAGAG GAAGTCTTCA CCATGCCAGA GAGAAAAGTG
    AAACCTCCCA TAGCCGAGAA ACCTCCATTG GCCCGAAGGC CTCCAAGTTT GGTCCACAGG
    CCGCCCTCTC TCCCTGGGGA GTATCCACTA ACTTCTCCTA CTATGGCTAT GGCATCCAGG
    AGCTCCATTC CACACATGAA GCAGCTTCCC CAAGACAGCT ACACAGTGTT GCGGAAACCA
    AAGTCACCCA GCTTCCCCGG GGAGTCAACA GCATCCTCCA GCCTTGTCCT CTCACCTCTT
    GCCAGTTCCT CTGGTGCTTT CTTCTCAGGA ACACAGCAAC CTCCCCAGGC CAGTGTAGAA
    GACGGGGGCC CCAAGGTGAG AGCCCTGCCT GAAAGAATCG GCCTCCAGAG CCAGGAAGAA
    GCTGAGAAAA AGATGACCAA GATTCCACCT CCGGTACCAA AAAAGCCCAG TGTGCTCTAC
    CTGCCTCTCA CCTCCCCTGT AGCTCAAATG GATGCCTGCA TGGCAGAACC AAGGCTACCT
    TTCAGCCCCA TCATCACCCT GGAGGAAGAT GGCAAGTGTC CCTCCACTGG TGATGACCAG
    AAGTCACCTG GTAAAGGGGT GACTTCGCCT CTTCACACTG ACACAGAAAA AGAGGCCATC
    TCTCCAGGGA GGTCAGTGGA ACCGAGTGCT GAAGAGAAAA GTTTAATCAG TGATAAAACA
    GCCGAATGGA TTGCAGAGGA GGAGGATGAC GTGTTTGTGG CTTCCCGCAC AACTGAAGAT
    TTATTTACTG TGATACACAG GTCCAAAAGA AAGCTACTGG GCTGGAAAGA GACTGGGGAG
    GGCTTCACGG GAAGCAAACC CAGCTCCCAT TCACCAGTGA AGAATACAGC TGATTCTCCC
    ACCGGTGAGG CTGCTGCTGC CCCAGGGCCA AGTAGCAGTG CCTGCCTAGA TGCTGGTAGA
    AATGATGATT TCAAGGCCTT GCTCCAGAAG AAGGGAAGTA AGGCAACTCC CAGGACCCGC
    CCCTCAGCGG CCGAGCTGCT GAAGACCACT AACCCTCTGG CTCGAAGGAT TATTGCACAG
    TTCTCAAAAG ACTACGAACC CACTGACAAC CCCAGTACCT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu13929
    Clone ID Related Accession (Same CDS sequence) NM_001163610.1
    Accession Version NM_001163610.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3660bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-27
    Organism Mus musculus(house mouse)
    Product NHS-like protein 2
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AL808026.10 and AL807784.11. On or before Jul 26, 2009 this sequence version replaced XR_031588.1, XM_919948.2, XM_896183.2, XM_001478287.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##RefSeq-Attributes-START## inferred exon combination :: based on alignments, homology ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGCCGTTCT ACCGGCGCAC TGTGGTGCCC CAGCGTCTGT GCCCGCGCAA CCCTCCGCAG 
    CCGCTGGCTG AGCTCCGCGA CGTGAGCCAC CTGGCCGCGC TCAGTCTGCT CCGCCAGCTC
    GCAGACCTCT GCGGCCACTC GCTGGCTCTG CTGGAGGACC TCGAGGGGCA CCTGCTGGCT
    CTGGGGCGCC GCACCGACAG CCTGTACCGG CGCACCGTCC GGCTCCGCCG CCGCCTTCCC
    TGTCGCCTGC TTGGCCCGGA GGATGATGAA GAATTGCTAG CTGCAGCTAA CTCGGGTCGG
    GAAAATGCGA CAGCGACTGC CCACTCGAGG TCGTCATGGC GACAGCCAGT GAACGTGTTC
    CTCTCCTCGG GCAGGCCCCC GAGTGTAGAG GAACTGCTTC GCGAGGCGCA GCTCAATCTC
    CAGAGCCTGT TGCAAGAAGA ATATGAGGAA CAGTATTCGG AGGCCAGACT TCTGGGGCAG
    ACCTTCCGCT CTTCTGATGG GGCCCCTGAG CCTACTCCCA GCCCAAGGCC CCAGTCTGCC
    AAGCGTCTGG AGTTTGTACT GATGCCTGCA AAACGGCAGC TGAGCGAGGA TGAGACCACG
    ACCCAGGGTG TGAGGGCCCC TGAGGCCTGC CTGAGCCTGT CTACTGCCAA CAAGCAAAGT
    GCCTGGAATG ACCCCTTCCC TCTACCCATC CTAAAGGAGA GGCTCTGGCT TCAGCCCTGC
    TCCACACAGT CTGACCTTGT GCCAATCAAC ATCTCTGGGC AGCAGTTTGA TAGACATGCA
    AGTTTTCGAC ACTCGTTGTT TAACACAGAG ACAGCCGTGA ACCCCAAGTC CACCCTGAGG
    CGGAGGCGGA CCATTATTGG ATTCTCTAAC TTTTCTCAGC GAGACCAAGG TCACAGCAGC
    AGCCCCACAG GCAGCCTGGC CCGCTCTGCC ACCTCAGACA TCAGGCCTGG CCACTCAGCT
    CCACAAGTTG TTCAGGGAAG AGTTGCTGTT GGGCAGGAAG CTCGGTTCCC AAGTCTCACC
    TCACCAGGAC TGAGACACTC TTCTAGTGAG CCCGGAGACG CTCACCAGGC ACGCAGTGCC
    TCAGACCATC CCGGCATGGA GAGCATGGCA GTGGTGTACA GTGTCCCCAG TTCTTGCAAT
    GGACCAACAG AGTCAACATT CTCCACTTCC TGGAAGGGAG ATGCTTTCAC ATACATGACC
    CCAAGTGCCA GCAGCCAGGG CAATCAAGTC AGTGAAAATG GAAAAAACCC TTCCTCGGGC
    AATTCTTGGG TCCCTCTGAA CACACTCCCA CCTCTGGTTC CTAAGGAGGC GGCCACGCTC
    TTTGTCACCC GTGATAACCC AGCAGGATGC ACTGGGCTAC CCAGCTACTC TGAGCACCCC
    ACTCTACGAA GACAGATACC AGAAAGACCT CCCAAGATTG GCCTTCTTGC CCGTGGTACC
    TCAAGGCTGG AAACAGGCCC AGGTGGGACC AACAGGTTCC GGGAGCGGTC ACTGTCTGTA
    CCCACAGACT CGGGTGTTAC CTCAGTGGAC TATGACGAAG AACAGAAGAC CAGTGAGACC
    CGCATCCTGC CTTATGCCAG TACAAGCTCC GAGGGCAGTA ACAGTACTGA CAACATTGCA
    GCCCTCAGCA CTGAGCAGGA GGCACGGCAC AGAAGGCAAA GATCCAAAAG TATTTCACTC
    AAGAAGGCCA AAAAGAAGCC CTCTCCACCC ATGCGAAGTG TCTCACTCGT CAAAGATGAG
    CCAGCCCTCC CACCAGAAGG CGAATTGGTA CTGCCCAAGG ACCAGAGGCC TAGGAGCCTT
    TGCCTCTCCT TGGAACACCA AGGGCACCAC CCACCTCACC CAGATGCTCA AGGTCACCCA
    GCTGTGCCAA TGCTCAAAGA TCCAGGAAGT ACGCAGTTCT CTCACCACTG GTATCTTACT
    GACTGGAAGT CTGGTGACAC CTACCAGTCC TTGTCCAGCT CCAGCACTGC CACCGGCACC
    ACGGTCATTG AGTGCACTCA AGTTCAGGGA AGCTCAGAGT CTCTGGCCTC CCCTTCCACC
    TCCAGAGCGA CAACACCCTC CCAGCTCTCC ATTGAGGTGG AAGCCAGGGA AGTAGCCTCT
    CCTGGGAGGC CCACTGGACT GATGTCCCCT TCCAGTGGTT ACTCCAGCCA GTCAGAGACA
    CCCACACCCA CTGTCTCCAT GTCCTTGACC CTGGGCCACT TACCTCCTCC AAGTGCTAGT
    GTCAGAGTAC GTCCAGTGGT ACCAGAAAGG AAGTCATCAC TGCCCCCAAC ATCACCAATG
    GAGAAAATCT GCAAGTCACG GTTATCATTT GACCTACCAC TGACCTCTTC AACCACCCTG
    GATCTGTCGG GGATGAGTAT CTCCATCCGC AGCAAAACCA AGGTGAGCCG CCATCACTCT
    GATACCAATT TTGGGGTCAA ACTGGCACAG AAAACTAGTC CAAACCAACC GATCATGCCC
    ATGGTTACTC AGTCTGACCT CCGTTCTGTT CGCCTGAGGT CAGTCAGCAA GTCTGAGCCA
    GAAGACGACA TTGAGAGTCC AGATTACATA GAGGAACCTG GAGCAGAGGA AGTCTTCACC
    ATGCCAGAGA GAAAAGTGAA ACCTCCCATA GCCGAGAAAC CTCCATTGGC CCGAAGGCCT
    CCAAGTTTGG TCCACAGGCC GCCCTCTCTC CCTGGGGAGT ATCCACTAAC TTCTCCTACT
    ATGGCTATGG CATCCAGGAG CTCCATTCCA CACATGAAGC AGCTTCCCCA AGACAGCTAC
    ACAGTGTTGC GGAAACCAAA GTCACCCAGC TTCCCCGGGG AGTCAACAGC ATCCTCCAGC
    CTTGTCCTCT CACCTCTTGC CAGTTCCTCT GGTGCTTTCT TCTCAGGAAC ACAGCAACCT
    CCCCAGGCCA GTGTAGAAGA CGGGGGCCCC AAGGTGAGAG CCCTGCCTGA AAGAATCGGC
    CTCCAGAGCC AGGAAGAAGC TGAGAAAAAG ATGACCAAGA TTCCACCTCC GGTACCAAAA
    AAGCCCAGTG TGCTCTACCT GCCTCTCACC TCCCCTGTAG CTCAAATGGA TGCCTGCATG
    GCAGAACCAA GGCTACCTTT CAGCCCCATC ATCACCCTGG AGGAAGATGG CAAGTGTCCC
    TCCACTGGTG ATGACCAGAA GTCACCTGGT AAAGGGGTGA CTTCGCCTCT TCACACTGAC
    ACAGAAAAAG AGGCCATCTC TCCAGGGAGG TCAGTGGAAC CGAGTGCTGA AGAGAAAAGT
    TTAATCAGTG ATAAAACAGC CGAATGGATT GCAGAGGAGG AGGATGACGT GTTTGTGGCT
    TCCCGCACAA CTGAAGATTT ATTTACTGTG ATACACAGGT CCAAAAGAAA GCTACTGGGC
    TGGAAAGAGA CTGGGGAGGG CTTCACGGGA AGCAAACCCA GCTCCCATTC ACCAGTGAAG
    AATACAGCTG ATTCTCCCAC CGGTGAGGCT GCTGCTGCCC CAGGGCCAAG TAGCAGTGCC
    TGCCTAGATG CTGGTAGAAA TGATGATTTC AAGGCCTTGC TCCAGAAGAA GGGAAGTAAG
    GCAACTCCCA GGACCCGCCC CTCAGCGGCC GAGCTGCTGA AGACCACTAA CCCTCTGGCT
    CGAAGGATTA TTGCACAGTT CTCAAAAGAC TACGAACCCA CTGACAACCC CAGTACCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001157082.1
    CDS184..3843
    Translation

    Target ORF information:

    RefSeq Version NM_001163610.1
    Organism Mus musculus(house mouse)
    Definition Mus musculus NHS-like 2 (Nhsl2), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001163610.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGCCGTTCT ACCGGCGCAC TGTGGTGCCC CAGCGTCTGT GCCCGCGCAA CCCTCCGCAG 
    CCGCTGGCTG AGCTCCGCGA CGTGAGCCAC CTGGCCGCGC TCAGTCTGCT CCGCCAGCTC
    GCAGACCTCT GCGGCCACTC GCTGGCTCTG CTGGAGGACC TCGAGGGGCA CCTGCTGGCT
    CTGGGGCGCC GCACCGACAG CCTGTACCGG CGCACCGTCC GGCTCCGCCG CCGCCTTCCC
    TGTCGCCTGC TTGGCCCGGA GGATGATGAA GAATTGCTAG CTGCAGCTAA CTCGGGTCGG
    GAAAATGCGA CAGCGACTGC CCACTCGAGG TCGTCATGGC GACAGCCAGT GAACGTGTTC
    CTCTCCTCGG GCAGGCCCCC GAGTGTAGAG GAACTGCTTC GCGAGGCGCA GCTCAATCTC
    CAGAGCCTGT TGCAAGAAGA ATATGAGGAA CAGTATTCGG AGGCCAGACT TCTGGGGCAG
    ACCTTCCGCT CTTCTGATGG GGCCCCTGAG CCTACTCCCA GCCCAAGGCC CCAGTCTGCC
    AAGCGTCTGG AGTTTGTACT GATGCCTGCA AAACGGCAGC TGAGCGAGGA TGAGACCACG
    ACCCAGGGTG TGAGGGCCCC TGAGGCCTGC CTGAGCCTGT CTACTGCCAA CAAGCAAAGT
    GCCTGGAATG ACCCCTTCCC TCTACCCATC CTAAAGGAGA GGCTCTGGCT TCAGCCCTGC
    TCCACACAGT CTGACCTTGT GCCAATCAAC ATCTCTGGGC AGCAGTTTGA TAGACATGCA
    AGTTTTCGAC ACTCGTTGTT TAACACAGAG ACAGCCGTGA ACCCCAAGTC CACCCTGAGG
    CGGAGGCGGA CCATTATTGG ATTCTCTAAC TTTTCTCAGC GAGACCAAGG TCACAGCAGC
    AGCCCCACAG GCAGCCTGGC CCGCTCTGCC ACCTCAGACA TCAGGCCTGG CCACTCAGCT
    CCACAAGTTG TTCAGGGAAG AGTTGCTGTT GGGCAGGAAG CTCGGTTCCC AAGTCTCACC
    TCACCAGGAC TGAGACACTC TTCTAGTGAG CCCGGAGACG CTCACCAGGC ACGCAGTGCC
    TCAGACCATC CCGGCATGGA GAGCATGGCA GTGGTGTACA GTGTCCCCAG TTCTTGCAAT
    GGACCAACAG AGTCAACATT CTCCACTTCC TGGAAGGGAG ATGCTTTCAC ATACATGACC
    CCAAGTGCCA GCAGCCAGGG CAATCAAGTC AGTGAAAATG GAAAAAACCC TTCCTCGGGC
    AATTCTTGGG TCCCTCTGAA CACACTCCCA CCTCTGGTTC CTAAGGAGGC GGCCACGCTC
    TTTGTCACCC GTGATAACCC AGCAGGATGC ACTGGGCTAC CCAGCTACTC TGAGCACCCC
    ACTCTACGAA GACAGATACC AGAAAGACCT CCCAAGATTG GCCTTCTTGC CCGTGGTACC
    TCAAGGCTGG AAACAGGCCC AGGTGGGACC AACAGGTTCC GGGAGCGGTC ACTGTCTGTA
    CCCACAGACT CGGGTGTTAC CTCAGTGGAC TATGACGAAG AACAGAAGAC CAGTGAGACC
    CGCATCCTGC CTTATGCCAG TACAAGCTCC GAGGGCAGTA ACAGTACTGA CAACATTGCA
    GCCCTCAGCA CTGAGCAGGA GGCACGGCAC AGAAGGCAAA GATCCAAAAG TATTTCACTC
    AAGAAGGCCA AAAAGAAGCC CTCTCCACCC ATGCGAAGTG TCTCACTCGT CAAAGATGAG
    CCAGCCCTCC CACCAGAAGG CGAATTGGTA CTGCCCAAGG ACCAGAGGCC TAGGAGCCTT
    TGCCTCTCCT TGGAACACCA AGGGCACCAC CCACCTCACC CAGATGCTCA AGGTCACCCA
    GCTGTGCCAA TGCTCAAAGA TCCAGGAAGT ACGCAGTTCT CTCACCACTG GTATCTTACT
    GACTGGAAGT CTGGTGACAC CTACCAGTCC TTGTCCAGCT CCAGCACTGC CACCGGCACC
    ACGGTCATTG AGTGCACTCA AGTTCAGGGA AGCTCAGAGT CTCTGGCCTC CCCTTCCACC
    TCCAGAGCGA CAACACCCTC CCAGCTCTCC ATTGAGGTGG AAGCCAGGGA AGTAGCCTCT
    CCTGGGAGGC CCACTGGACT GATGTCCCCT TCCAGTGGTT ACTCCAGCCA GTCAGAGACA
    CCCACACCCA CTGTCTCCAT GTCCTTGACC CTGGGCCACT TACCTCCTCC AAGTGCTAGT
    GTCAGAGTAC GTCCAGTGGT ACCAGAAAGG AAGTCATCAC TGCCCCCAAC ATCACCAATG
    GAGAAAATCT GCAAGTCACG GTTATCATTT GACCTACCAC TGACCTCTTC AACCACCCTG
    GATCTGTCGG GGATGAGTAT CTCCATCCGC AGCAAAACCA AGGTGAGCCG CCATCACTCT
    GATACCAATT TTGGGGTCAA ACTGGCACAG AAAACTAGTC CAAACCAACC GATCATGCCC
    ATGGTTACTC AGTCTGACCT CCGTTCTGTT CGCCTGAGGT CAGTCAGCAA GTCTGAGCCA
    GAAGACGACA TTGAGAGTCC AGATTACATA GAGGAACCTG GAGCAGAGGA AGTCTTCACC
    ATGCCAGAGA GAAAAGTGAA ACCTCCCATA GCCGAGAAAC CTCCATTGGC CCGAAGGCCT
    CCAAGTTTGG TCCACAGGCC GCCCTCTCTC CCTGGGGAGT ATCCACTAAC TTCTCCTACT
    ATGGCTATGG CATCCAGGAG CTCCATTCCA CACATGAAGC AGCTTCCCCA AGACAGCTAC
    ACAGTGTTGC GGAAACCAAA GTCACCCAGC TTCCCCGGGG AGTCAACAGC ATCCTCCAGC
    CTTGTCCTCT CACCTCTTGC CAGTTCCTCT GGTGCTTTCT TCTCAGGAAC ACAGCAACCT
    CCCCAGGCCA GTGTAGAAGA CGGGGGCCCC AAGGTGAGAG CCCTGCCTGA AAGAATCGGC
    CTCCAGAGCC AGGAAGAAGC TGAGAAAAAG ATGACCAAGA TTCCACCTCC GGTACCAAAA
    AAGCCCAGTG TGCTCTACCT GCCTCTCACC TCCCCTGTAG CTCAAATGGA TGCCTGCATG
    GCAGAACCAA GGCTACCTTT CAGCCCCATC ATCACCCTGG AGGAAGATGG CAAGTGTCCC
    TCCACTGGTG ATGACCAGAA GTCACCTGGT AAAGGGGTGA CTTCGCCTCT TCACACTGAC
    ACAGAAAAAG AGGCCATCTC TCCAGGGAGG TCAGTGGAAC CGAGTGCTGA AGAGAAAAGT
    TTAATCAGTG ATAAAACAGC CGAATGGATT GCAGAGGAGG AGGATGACGT GTTTGTGGCT
    TCCCGCACAA CTGAAGATTT ATTTACTGTG ATACACAGGT CCAAAAGAAA GCTACTGGGC
    TGGAAAGAGA CTGGGGAGGG CTTCACGGGA AGCAAACCCA GCTCCCATTC ACCAGTGAAG
    AATACAGCTG ATTCTCCCAC CGGTGAGGCT GCTGCTGCCC CAGGGCCAAG TAGCAGTGCC
    TGCCTAGATG CTGGTAGAAA TGATGATTTC AAGGCCTTGC TCCAGAAGAA GGGAAGTAAG
    GCAACTCCCA GGACCCGCCC CTCAGCGGCC GAGCTGCTGA AGACCACTAA CCCTCTGGCT
    CGAAGGATTA TTGCACAGTT CTCAAAAGAC TACGAACCCA CTGACAACCC CAGTACCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu56077
    Clone ID Related Accession (Same CDS sequence) XM_006527718.4 , XM_006527718.3
    Accession Version XM_006527718.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3450bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-21
    Organism Mus musculus(house mouse)
    Product NHS-like protein 2 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000086.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006527718.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGATGGGCA ACTCTCACCA CAAGCAACCG AGGAGTAAGA GCCAAAGCAG CAGGATGCAT 
    TCAGCAACAG CTGCAGCTAA CTCGGGTCGG GAAAATGCGA CAGCGACTGC CCACTCGAGG
    TCGTCATGGC GACAGCCAGT GAACGTGTTC CTCTCCTCGG GCAGGCCCCC GAGTGTAGAG
    GAACTGCTTC GCGAGGCGCA GCTCAATCTC CAGAGCCTGT TGCAAGAAGA ATATGAGGAA
    CAGTATTCGG AGGCCAGACT TCTGGGGCAG ACCTTCCGCT CTTCTGATGG GGCCCCTGAG
    CCTACTCCCA GCCCAAGGCC CCAGTCTGCC AAGCGTCTGG AGTTTGTACT GATGCCTGCA
    AAACGGCAGC TGAGCGAGGA TGAGACCACG ACCCAGGGTG TGAGGGCCCC TGAGGCCTGC
    CTGAGCCTGT CTACTGCCAA CAAGCAAAGT GCCTGGAATG ACCCCTTCCC TCTACCCATC
    CTAAAGGAGA GGCTCTGGCT TCAGCCCTGC TCCACACAGT CTGACCTTGT GCCAATCAAC
    ATCTCTGGGC AGCAGTTTGA TAGACATGCA AGTTTTCGAC ACTCGTTGTT TAACACAGAG
    ACAGCCGTGA ACCCCAAGTC CACCCTGAGG CGGAGGCGGA CCATTATTGG ATTCTCTAAC
    TTTTCTCAGC GAGACCAAGG TCACAGCAGC AGCCCCACAG GCAGCCTGGC CCGCTCTGCC
    ACCTCAGACA TCAGGCCTGG CCACTCAGCT CCACAAGTTG TTCAGGGAAG AGTTGCTGTT
    GGGCAGGAAG CTCGGTTCCC AAGTCTCACC TCACCAGGAC TGAGACACTC TTCTAGTGAG
    CCCGGAGACG CTCACCAGGC ACGCAGTGCC TCAGACCATC CCGGCATGGA GAGCATGGCA
    GTGGTGTACA GTGTCCCCAG TTCTTGCAAT GGACCAACAG AGTCAACATT CTCCACTTCC
    TGGAAGGGAG ATGCTTTCAC ATACATGACC CCAAGTGCCA GCAGCCAGGG CAATCAAGTC
    AGTGAAAATG GAAAAAACCC TTCCTCGGGC AATTCTTGGG TCCCTCTGAA CACACTCCCA
    CCTCTGGTTC CTAAGGAGGC GGCCACGCTC TTTGTCACCC GTGATAACCC AGCAGGATGC
    ACTGGGCTAC CCAGCTACTC TGAGCACCCC ACTCTACGAA GACAGATACC AGAAAGACCT
    CCCAAGATTG GCCTTCTTGC CCGTGGTACC TCAAGGCTGG AAACAGGCCC AGGTGGGACC
    AACAGGTTCC GGGAGCGGTC ACTGTCTGTA CCCACAGACT CGGGTGTTAC CTCAGTGGAC
    TATGACGAAG AACAGAAGAC CAGTGAGACC CGCATCCTGC CTTATGCCAG TACAAGCTCC
    GAGGGCAGTA ACAGTACTGA CAACATTGCA GCCCTCAGCA CTGAGCAGGA GGCACGGCAC
    AGAAGGCAAA GATCCAAAAG TATTTCACTC AAGAAGGCCA AAAAGAAGCC CTCTCCACCC
    ATGCGAAGTG TCTCACTCGT CAAAGATGAG CCAGCCCTCC CACCAGAAGG CGAATTGGTA
    CTGCCCAAGG ACCAGAGGCC TAGGAGCCTT TGCCTCTCCT TGGAACACCA AGGGCACCAC
    CCACCTCACC CAGATGCTCA AGGTCACCCA GCTGTGCCAA TGCTCAAAGA TCCAGGAAGT
    ACGCAGTTCT CTCACCACTG GTATCTTACT GACTGGAAGT CTGGTGACAC CTACCAGTCC
    TTGTCCAGCT CCAGCACTGC CACCGGCACC ACGGTCATTG AGTGCACTCA AGTTCAGGGA
    AGCTCAGAGT CTCTGGCCTC CCCTTCCACC TCCAGAGCGA CAACACCCTC CCAGCTCTCC
    ATTGAGGTGG AAGCCAGGGA AGTAGCCTCT CCTGGGAGGC CCACTGGACT GATGTCCCCT
    TCCAGTGGTT ACTCCAGCCA GTCAGAGACA CCCACACCCA CTGTCTCCAT GTCCTTGACC
    CTGGGCCACT TACCTCCTCC AAGTGCTAGT GTCAGAGTAC GTCCAGTGGT ACCAGAAAGG
    AAGTCATCAC TGCCCCCAAC ATCACCAATG GAGAAAATCT GCAAGTCACG GTTATCATTT
    GACCTACCAC TGACCTCTTC AACCACCCTG GATCTGTCGG GGATGAGTAT CTCCATCCGC
    AGCAAAACCA AGGTGAGCCG CCATCACTCT GATACCAATT TTGGGGTCAA ACTGGCACAG
    AAAACTAGTC CAAACCAACC GATCATGCCC ATGGTTACTC AGTCTGACCT CCGTTCTGTT
    CGCCTGAGGT CAGTCAGCAA GTCTGAGCCA GAAGACGACA TTGAGAGTCC AGATTACATA
    GAGGAACCTG GAGCAGAGGA AGTCTTCACC ATGCCAGAGA GAAAAGTGAA ACCTCCCATA
    GCCGAGAAAC CTCCATTGGC CCGAAGGCCT CCAAGTTTGG TCCACAGGCC GCCCTCTCTC
    CCTGGGGAGT ATCCACTAAC TTCTCCTACT ATGGCTATGG CATCCAGGAG CTCCATTCCA
    CACATGAAGC AGCTTCCCCA AGACAGCTAC ACAGTGTTGC GGAAACCAAA GTCACCCAGC
    TTCCCCGGGG AGTCAACAGC ATCCTCCAGC CTTGTCCTCT CACCTCTTGC CAGTTCCTCT
    GGTGCTTTCT TCTCAGGAAC ACAGCAACCT CCCCAGGCCA GTGTAGAAGA CGGGGGCCCC
    AAGGTGAGAG CCCTGCCTGA AAGAATCGGC CTCCAGAGCC AGGAAGAAGC TGAGAAAAAG
    ATGACCAAGA TTCCACCTCC GGTACCAAAA AAGCCCAGTG TGCTCTACCT GCCTCTCACC
    TCCCCTGTAG CTCAAATGGA TGCCTGCATG GCAGAACCAA GGCTACCTTT CAGCCCCATC
    ATCACCCTGG AGGAAGATGG CAAGTGTCCC TCCACTGGTG ATGACCAGAA GTCACCTGGT
    AAAGGGGTGA CTTCGCCTCT TCACACTGAC ACAGAAAAAG AGGCCATCTC TCCAGGGAGG
    TCAGTGGAAC CGAGTGCTGA AGAGAAAAGT TTAATCAGTG ATAAAACAGC CGAATGGATT
    GCAGAGGAGG AGGATGACGT GTTTGTGGCT TCCCGCACAA CTGAAGATTT ATTTACTGTG
    ATACACAGGT CCAAAAGAAA GCTACTGGGC TGGAAAGAGA CTGGGGAGGG CTTCACGGGA
    AGCAAACCCA GCTCCCATTC ACCAGTGAAG AATACAGCTG ATTCTCCCAC CGGTGAGGCT
    GCTGCTGCCC CAGGGCCAAG TAGCAGTGCC TGCCTAGATG CTGGTAGAAA TGATGATTTC
    AAGGCCTTGC TCCAGAAGAA GGGAAGTAAG GCAACTCCCA GGACCCGCCC CTCAGCGGCC
    GAGCTGCTGA AGACCACTAA CCCTCTGGCT CGAAGGATTA TTGCACAGTT CTCAAAAGAC
    TACGAACCCA CTGACAACCC CAGTACCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006527781.1
    CDS278..3727
    Translation

    Target ORF information:

    RefSeq Version XM_006527718.3
    Organism Mus musculus(house mouse)
    Definition Mus musculus NHS-like 2 (Nhsl2), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006527718.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGATGGGCA ACTCTCACCA CAAGCAACCG AGGAGTAAGA GCCAAAGCAG CAGGATGCAT 
    TCAGCAACAG CTGCAGCTAA CTCGGGTCGG GAAAATGCGA CAGCGACTGC CCACTCGAGG
    TCGTCATGGC GACAGCCAGT GAACGTGTTC CTCTCCTCGG GCAGGCCCCC GAGTGTAGAG
    GAACTGCTTC GCGAGGCGCA GCTCAATCTC CAGAGCCTGT TGCAAGAAGA ATATGAGGAA
    CAGTATTCGG AGGCCAGACT TCTGGGGCAG ACCTTCCGCT CTTCTGATGG GGCCCCTGAG
    CCTACTCCCA GCCCAAGGCC CCAGTCTGCC AAGCGTCTGG AGTTTGTACT GATGCCTGCA
    AAACGGCAGC TGAGCGAGGA TGAGACCACG ACCCAGGGTG TGAGGGCCCC TGAGGCCTGC
    CTGAGCCTGT CTACTGCCAA CAAGCAAAGT GCCTGGAATG ACCCCTTCCC TCTACCCATC
    CTAAAGGAGA GGCTCTGGCT TCAGCCCTGC TCCACACAGT CTGACCTTGT GCCAATCAAC
    ATCTCTGGGC AGCAGTTTGA TAGACATGCA AGTTTTCGAC ACTCGTTGTT TAACACAGAG
    ACAGCCGTGA ACCCCAAGTC CACCCTGAGG CGGAGGCGGA CCATTATTGG ATTCTCTAAC
    TTTTCTCAGC GAGACCAAGG TCACAGCAGC AGCCCCACAG GCAGCCTGGC CCGCTCTGCC
    ACCTCAGACA TCAGGCCTGG CCACTCAGCT CCACAAGTTG TTCAGGGAAG AGTTGCTGTT
    GGGCAGGAAG CTCGGTTCCC AAGTCTCACC TCACCAGGAC TGAGACACTC TTCTAGTGAG
    CCCGGAGACG CTCACCAGGC ACGCAGTGCC TCAGACCATC CCGGCATGGA GAGCATGGCA
    GTGGTGTACA GTGTCCCCAG TTCTTGCAAT GGACCAACAG AGTCAACATT CTCCACTTCC
    TGGAAGGGAG ATGCTTTCAC ATACATGACC CCAAGTGCCA GCAGCCAGGG CAATCAAGTC
    AGTGAAAATG GAAAAAACCC TTCCTCGGGC AATTCTTGGG TCCCTCTGAA CACACTCCCA
    CCTCTGGTTC CTAAGGAGGC GGCCACGCTC TTTGTCACCC GTGATAACCC AGCAGGATGC
    ACTGGGCTAC CCAGCTACTC TGAGCACCCC ACTCTACGAA GACAGATACC AGAAAGACCT
    CCCAAGATTG GCCTTCTTGC CCGTGGTACC TCAAGGCTGG AAACAGGCCC AGGTGGGACC
    AACAGGTTCC GGGAGCGGTC ACTGTCTGTA CCCACAGACT CGGGTGTTAC CTCAGTGGAC
    TATGACGAAG AACAGAAGAC CAGTGAGACC CGCATCCTGC CTTATGCCAG TACAAGCTCC
    GAGGGCAGTA ACAGTACTGA CAACATTGCA GCCCTCAGCA CTGAGCAGGA GGCACGGCAC
    AGAAGGCAAA GATCCAAAAG TATTTCACTC AAGAAGGCCA AAAAGAAGCC CTCTCCACCC
    ATGCGAAGTG TCTCACTCGT CAAAGATGAG CCAGCCCTCC CACCAGAAGG CGAATTGGTA
    CTGCCCAAGG ACCAGAGGCC TAGGAGCCTT TGCCTCTCCT TGGAACACCA AGGGCACCAC
    CCACCTCACC CAGATGCTCA AGGTCACCCA GCTGTGCCAA TGCTCAAAGA TCCAGGAAGT
    ACGCAGTTCT CTCACCACTG GTATCTTACT GACTGGAAGT CTGGTGACAC CTACCAGTCC
    TTGTCCAGCT CCAGCACTGC CACCGGCACC ACGGTCATTG AGTGCACTCA AGTTCAGGGA
    AGCTCAGAGT CTCTGGCCTC CCCTTCCACC TCCAGAGCGA CAACACCCTC CCAGCTCTCC
    ATTGAGGTGG AAGCCAGGGA AGTAGCCTCT CCTGGGAGGC CCACTGGACT GATGTCCCCT
    TCCAGTGGTT ACTCCAGCCA GTCAGAGACA CCCACACCCA CTGTCTCCAT GTCCTTGACC
    CTGGGCCACT TACCTCCTCC AAGTGCTAGT GTCAGAGTAC GTCCAGTGGT ACCAGAAAGG
    AAGTCATCAC TGCCCCCAAC ATCACCAATG GAGAAAATCT GCAAGTCACG GTTATCATTT
    GACCTACCAC TGACCTCTTC AACCACCCTG GATCTGTCGG GGATGAGTAT CTCCATCCGC
    AGCAAAACCA AGGTGAGCCG CCATCACTCT GATACCAATT TTGGGGTCAA ACTGGCACAG
    AAAACTAGTC CAAACCAACC GATCATGCCC ATGGTTACTC AGTCTGACCT CCGTTCTGTT
    CGCCTGAGGT CAGTCAGCAA GTCTGAGCCA GAAGACGACA TTGAGAGTCC AGATTACATA
    GAGGAACCTG GAGCAGAGGA AGTCTTCACC ATGCCAGAGA GAAAAGTGAA ACCTCCCATA
    GCCGAGAAAC CTCCATTGGC CCGAAGGCCT CCAAGTTTGG TCCACAGGCC GCCCTCTCTC
    CCTGGGGAGT ATCCACTAAC TTCTCCTACT ATGGCTATGG CATCCAGGAG CTCCATTCCA
    CACATGAAGC AGCTTCCCCA AGACAGCTAC ACAGTGTTGC GGAAACCAAA GTCACCCAGC
    TTCCCCGGGG AGTCAACAGC ATCCTCCAGC CTTGTCCTCT CACCTCTTGC CAGTTCCTCT
    GGTGCTTTCT TCTCAGGAAC ACAGCAACCT CCCCAGGCCA GTGTAGAAGA CGGGGGCCCC
    AAGGTGAGAG CCCTGCCTGA AAGAATCGGC CTCCAGAGCC AGGAAGAAGC TGAGAAAAAG
    ATGACCAAGA TTCCACCTCC GGTACCAAAA AAGCCCAGTG TGCTCTACCT GCCTCTCACC
    TCCCCTGTAG CTCAAATGGA TGCCTGCATG GCAGAACCAA GGCTACCTTT CAGCCCCATC
    ATCACCCTGG AGGAAGATGG CAAGTGTCCC TCCACTGGTG ATGACCAGAA GTCACCTGGT
    AAAGGGGTGA CTTCGCCTCT TCACACTGAC ACAGAAAAAG AGGCCATCTC TCCAGGGAGG
    TCAGTGGAAC CGAGTGCTGA AGAGAAAAGT TTAATCAGTG ATAAAACAGC CGAATGGATT
    GCAGAGGAGG AGGATGACGT GTTTGTGGCT TCCCGCACAA CTGAAGATTT ATTTACTGTG
    ATACACAGGT CCAAAAGAAA GCTACTGGGC TGGAAAGAGA CTGGGGAGGG CTTCACGGGA
    AGCAAACCCA GCTCCCATTC ACCAGTGAAG AATACAGCTG ATTCTCCCAC CGGTGAGGCT
    GCTGCTGCCC CAGGGCCAAG TAGCAGTGCC TGCCTAGATG CTGGTAGAAA TGATGATTTC
    AAGGCCTTGC TCCAGAAGAA GGGAAGTAAG GCAACTCCCA GGACCCGCCC CTCAGCGGCC
    GAGCTGCTGA AGACCACTAA CCCTCTGGCT CGAAGGATTA TTGCACAGTT CTCAAAAGAC
    TACGAACCCA CTGACAACCC CAGTACCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu56079
    Clone ID Related Accession (Same CDS sequence) XM_006527720.4 , XM_006527720.3
    Accession Version XM_006527720.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3423bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-21
    Organism Mus musculus(house mouse)
    Product NHS-like protein 2 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000086.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006527720.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGGAGAGAG GCGAGTCTCT TACCCTCTTC TGGAGCCGGG GAGCTGCAGC TAACTCGGGT 
    CGGGAAAATG CGACAGCGAC TGCCCACTCG AGGTCGTCAT GGCGACAGCC AGTGAACGTG
    TTCCTCTCCT CGGGCAGGCC CCCGAGTGTA GAGGAACTGC TTCGCGAGGC GCAGCTCAAT
    CTCCAGAGCC TGTTGCAAGA AGAATATGAG GAACAGTATT CGGAGGCCAG ACTTCTGGGG
    CAGACCTTCC GCTCTTCTGA TGGGGCCCCT GAGCCTACTC CCAGCCCAAG GCCCCAGTCT
    GCCAAGCGTC TGGAGTTTGT ACTGATGCCT GCAAAACGGC AGCTGAGCGA GGATGAGACC
    ACGACCCAGG GTGTGAGGGC CCCTGAGGCC TGCCTGAGCC TGTCTACTGC CAACAAGCAA
    AGTGCCTGGA ATGACCCCTT CCCTCTACCC ATCCTAAAGG AGAGGCTCTG GCTTCAGCCC
    TGCTCCACAC AGTCTGACCT TGTGCCAATC AACATCTCTG GGCAGCAGTT TGATAGACAT
    GCAAGTTTTC GACACTCGTT GTTTAACACA GAGACAGCCG TGAACCCCAA GTCCACCCTG
    AGGCGGAGGC GGACCATTAT TGGATTCTCT AACTTTTCTC AGCGAGACCA AGGTCACAGC
    AGCAGCCCCA CAGGCAGCCT GGCCCGCTCT GCCACCTCAG ACATCAGGCC TGGCCACTCA
    GCTCCACAAG TTGTTCAGGG AAGAGTTGCT GTTGGGCAGG AAGCTCGGTT CCCAAGTCTC
    ACCTCACCAG GACTGAGACA CTCTTCTAGT GAGCCCGGAG ACGCTCACCA GGCACGCAGT
    GCCTCAGACC ATCCCGGCAT GGAGAGCATG GCAGTGGTGT ACAGTGTCCC CAGTTCTTGC
    AATGGACCAA CAGAGTCAAC ATTCTCCACT TCCTGGAAGG GAGATGCTTT CACATACATG
    ACCCCAAGTG CCAGCAGCCA GGGCAATCAA GTCAGTGAAA ATGGAAAAAA CCCTTCCTCG
    GGCAATTCTT GGGTCCCTCT GAACACACTC CCACCTCTGG TTCCTAAGGA GGCGGCCACG
    CTCTTTGTCA CCCGTGATAA CCCAGCAGGA TGCACTGGGC TACCCAGCTA CTCTGAGCAC
    CCCACTCTAC GAAGACAGAT ACCAGAAAGA CCTCCCAAGA TTGGCCTTCT TGCCCGTGGT
    ACCTCAAGGC TGGAAACAGG CCCAGGTGGG ACCAACAGGT TCCGGGAGCG GTCACTGTCT
    GTACCCACAG ACTCGGGTGT TACCTCAGTG GACTATGACG AAGAACAGAA GACCAGTGAG
    ACCCGCATCC TGCCTTATGC CAGTACAAGC TCCGAGGGCA GTAACAGTAC TGACAACATT
    GCAGCCCTCA GCACTGAGCA GGAGGCACGG CACAGAAGGC AAAGATCCAA AAGTATTTCA
    CTCAAGAAGG CCAAAAAGAA GCCCTCTCCA CCCATGCGAA GTGTCTCACT CGTCAAAGAT
    GAGCCAGCCC TCCCACCAGA AGGCGAATTG GTACTGCCCA AGGACCAGAG GCCTAGGAGC
    CTTTGCCTCT CCTTGGAACA CCAAGGGCAC CACCCACCTC ACCCAGATGC TCAAGGTCAC
    CCAGCTGTGC CAATGCTCAA AGATCCAGGA AGTACGCAGT TCTCTCACCA CTGGTATCTT
    ACTGACTGGA AGTCTGGTGA CACCTACCAG TCCTTGTCCA GCTCCAGCAC TGCCACCGGC
    ACCACGGTCA TTGAGTGCAC TCAAGTTCAG GGAAGCTCAG AGTCTCTGGC CTCCCCTTCC
    ACCTCCAGAG CGACAACACC CTCCCAGCTC TCCATTGAGG TGGAAGCCAG GGAAGTAGCC
    TCTCCTGGGA GGCCCACTGG ACTGATGTCC CCTTCCAGTG GTTACTCCAG CCAGTCAGAG
    ACACCCACAC CCACTGTCTC CATGTCCTTG ACCCTGGGCC ACTTACCTCC TCCAAGTGCT
    AGTGTCAGAG TACGTCCAGT GGTACCAGAA AGGAAGTCAT CACTGCCCCC AACATCACCA
    ATGGAGAAAA TCTGCAAGTC ACGGTTATCA TTTGACCTAC CACTGACCTC TTCAACCACC
    CTGGATCTGT CGGGGATGAG TATCTCCATC CGCAGCAAAA CCAAGGTGAG CCGCCATCAC
    TCTGATACCA ATTTTGGGGT CAAACTGGCA CAGAAAACTA GTCCAAACCA ACCGATCATG
    CCCATGGTTA CTCAGTCTGA CCTCCGTTCT GTTCGCCTGA GGTCAGTCAG CAAGTCTGAG
    CCAGAAGACG ACATTGAGAG TCCAGATTAC ATAGAGGAAC CTGGAGCAGA GGAAGTCTTC
    ACCATGCCAG AGAGAAAAGT GAAACCTCCC ATAGCCGAGA AACCTCCATT GGCCCGAAGG
    CCTCCAAGTT TGGTCCACAG GCCGCCCTCT CTCCCTGGGG AGTATCCACT AACTTCTCCT
    ACTATGGCTA TGGCATCCAG GAGCTCCATT CCACACATGA AGCAGCTTCC CCAAGACAGC
    TACACAGTGT TGCGGAAACC AAAGTCACCC AGCTTCCCCG GGGAGTCAAC AGCATCCTCC
    AGCCTTGTCC TCTCACCTCT TGCCAGTTCC TCTGGTGCTT TCTTCTCAGG AACACAGCAA
    CCTCCCCAGG CCAGTGTAGA AGACGGGGGC CCCAAGGTGA GAGCCCTGCC TGAAAGAATC
    GGCCTCCAGA GCCAGGAAGA AGCTGAGAAA AAGATGACCA AGATTCCACC TCCGGTACCA
    AAAAAGCCCA GTGTGCTCTA CCTGCCTCTC ACCTCCCCTG TAGCTCAAAT GGATGCCTGC
    ATGGCAGAAC CAAGGCTACC TTTCAGCCCC ATCATCACCC TGGAGGAAGA TGGCAAGTGT
    CCCTCCACTG GTGATGACCA GAAGTCACCT GGTAAAGGGG TGACTTCGCC TCTTCACACT
    GACACAGAAA AAGAGGCCAT CTCTCCAGGG AGGTCAGTGG AACCGAGTGC TGAAGAGAAA
    AGTTTAATCA GTGATAAAAC AGCCGAATGG ATTGCAGAGG AGGAGGATGA CGTGTTTGTG
    GCTTCCCGCA CAACTGAAGA TTTATTTACT GTGATACACA GGTCCAAAAG AAAGCTACTG
    GGCTGGAAAG AGACTGGGGA GGGCTTCACG GGAAGCAAAC CCAGCTCCCA TTCACCAGTG
    AAGAATACAG CTGATTCTCC CACCGGTGAG GCTGCTGCTG CCCCAGGGCC AAGTAGCAGT
    GCCTGCCTAG ATGCTGGTAG AAATGATGAT TTCAAGGCCT TGCTCCAGAA GAAGGGAAGT
    AAGGCAACTC CCAGGACCCG CCCCTCAGCG GCCGAGCTGC TGAAGACCAC TAACCCTCTG
    GCTCGAAGGA TTATTGCACA GTTCTCAAAA GACTACGAAC CCACTGACAA CCCCAGTACC
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006527783.1
    CDS1918..5340
    Translation

    Target ORF information:

    RefSeq Version XM_006527720.3
    Organism Mus musculus(house mouse)
    Definition Mus musculus NHS-like 2 (Nhsl2), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006527720.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGGAGAGAG GCGAGTCTCT TACCCTCTTC TGGAGCCGGG GAGCTGCAGC TAACTCGGGT 
    CGGGAAAATG CGACAGCGAC TGCCCACTCG AGGTCGTCAT GGCGACAGCC AGTGAACGTG
    TTCCTCTCCT CGGGCAGGCC CCCGAGTGTA GAGGAACTGC TTCGCGAGGC GCAGCTCAAT
    CTCCAGAGCC TGTTGCAAGA AGAATATGAG GAACAGTATT CGGAGGCCAG ACTTCTGGGG
    CAGACCTTCC GCTCTTCTGA TGGGGCCCCT GAGCCTACTC CCAGCCCAAG GCCCCAGTCT
    GCCAAGCGTC TGGAGTTTGT ACTGATGCCT GCAAAACGGC AGCTGAGCGA GGATGAGACC
    ACGACCCAGG GTGTGAGGGC CCCTGAGGCC TGCCTGAGCC TGTCTACTGC CAACAAGCAA
    AGTGCCTGGA ATGACCCCTT CCCTCTACCC ATCCTAAAGG AGAGGCTCTG GCTTCAGCCC
    TGCTCCACAC AGTCTGACCT TGTGCCAATC AACATCTCTG GGCAGCAGTT TGATAGACAT
    GCAAGTTTTC GACACTCGTT GTTTAACACA GAGACAGCCG TGAACCCCAA GTCCACCCTG
    AGGCGGAGGC GGACCATTAT TGGATTCTCT AACTTTTCTC AGCGAGACCA AGGTCACAGC
    AGCAGCCCCA CAGGCAGCCT GGCCCGCTCT GCCACCTCAG ACATCAGGCC TGGCCACTCA
    GCTCCACAAG TTGTTCAGGG AAGAGTTGCT GTTGGGCAGG AAGCTCGGTT CCCAAGTCTC
    ACCTCACCAG GACTGAGACA CTCTTCTAGT GAGCCCGGAG ACGCTCACCA GGCACGCAGT
    GCCTCAGACC ATCCCGGCAT GGAGAGCATG GCAGTGGTGT ACAGTGTCCC CAGTTCTTGC
    AATGGACCAA CAGAGTCAAC ATTCTCCACT TCCTGGAAGG GAGATGCTTT CACATACATG
    ACCCCAAGTG CCAGCAGCCA GGGCAATCAA GTCAGTGAAA ATGGAAAAAA CCCTTCCTCG
    GGCAATTCTT GGGTCCCTCT GAACACACTC CCACCTCTGG TTCCTAAGGA GGCGGCCACG
    CTCTTTGTCA CCCGTGATAA CCCAGCAGGA TGCACTGGGC TACCCAGCTA CTCTGAGCAC
    CCCACTCTAC GAAGACAGAT ACCAGAAAGA CCTCCCAAGA TTGGCCTTCT TGCCCGTGGT
    ACCTCAAGGC TGGAAACAGG CCCAGGTGGG ACCAACAGGT TCCGGGAGCG GTCACTGTCT
    GTACCCACAG ACTCGGGTGT TACCTCAGTG GACTATGACG AAGAACAGAA GACCAGTGAG
    ACCCGCATCC TGCCTTATGC CAGTACAAGC TCCGAGGGCA GTAACAGTAC TGACAACATT
    GCAGCCCTCA GCACTGAGCA GGAGGCACGG CACAGAAGGC AAAGATCCAA AAGTATTTCA
    CTCAAGAAGG CCAAAAAGAA GCCCTCTCCA CCCATGCGAA GTGTCTCACT CGTCAAAGAT
    GAGCCAGCCC TCCCACCAGA AGGCGAATTG GTACTGCCCA AGGACCAGAG GCCTAGGAGC
    CTTTGCCTCT CCTTGGAACA CCAAGGGCAC CACCCACCTC ACCCAGATGC TCAAGGTCAC
    CCAGCTGTGC CAATGCTCAA AGATCCAGGA AGTACGCAGT TCTCTCACCA CTGGTATCTT
    ACTGACTGGA AGTCTGGTGA CACCTACCAG TCCTTGTCCA GCTCCAGCAC TGCCACCGGC
    ACCACGGTCA TTGAGTGCAC TCAAGTTCAG GGAAGCTCAG AGTCTCTGGC CTCCCCTTCC
    ACCTCCAGAG CGACAACACC CTCCCAGCTC TCCATTGAGG TGGAAGCCAG GGAAGTAGCC
    TCTCCTGGGA GGCCCACTGG ACTGATGTCC CCTTCCAGTG GTTACTCCAG CCAGTCAGAG
    ACACCCACAC CCACTGTCTC CATGTCCTTG ACCCTGGGCC ACTTACCTCC TCCAAGTGCT
    AGTGTCAGAG TACGTCCAGT GGTACCAGAA AGGAAGTCAT CACTGCCCCC AACATCACCA
    ATGGAGAAAA TCTGCAAGTC ACGGTTATCA TTTGACCTAC CACTGACCTC TTCAACCACC
    CTGGATCTGT CGGGGATGAG TATCTCCATC CGCAGCAAAA CCAAGGTGAG CCGCCATCAC
    TCTGATACCA ATTTTGGGGT CAAACTGGCA CAGAAAACTA GTCCAAACCA ACCGATCATG
    CCCATGGTTA CTCAGTCTGA CCTCCGTTCT GTTCGCCTGA GGTCAGTCAG CAAGTCTGAG
    CCAGAAGACG ACATTGAGAG TCCAGATTAC ATAGAGGAAC CTGGAGCAGA GGAAGTCTTC
    ACCATGCCAG AGAGAAAAGT GAAACCTCCC ATAGCCGAGA AACCTCCATT GGCCCGAAGG
    CCTCCAAGTT TGGTCCACAG GCCGCCCTCT CTCCCTGGGG AGTATCCACT AACTTCTCCT
    ACTATGGCTA TGGCATCCAG GAGCTCCATT CCACACATGA AGCAGCTTCC CCAAGACAGC
    TACACAGTGT TGCGGAAACC AAAGTCACCC AGCTTCCCCG GGGAGTCAAC AGCATCCTCC
    AGCCTTGTCC TCTCACCTCT TGCCAGTTCC TCTGGTGCTT TCTTCTCAGG AACACAGCAA
    CCTCCCCAGG CCAGTGTAGA AGACGGGGGC CCCAAGGTGA GAGCCCTGCC TGAAAGAATC
    GGCCTCCAGA GCCAGGAAGA AGCTGAGAAA AAGATGACCA AGATTCCACC TCCGGTACCA
    AAAAAGCCCA GTGTGCTCTA CCTGCCTCTC ACCTCCCCTG TAGCTCAAAT GGATGCCTGC
    ATGGCAGAAC CAAGGCTACC TTTCAGCCCC ATCATCACCC TGGAGGAAGA TGGCAAGTGT
    CCCTCCACTG GTGATGACCA GAAGTCACCT GGTAAAGGGG TGACTTCGCC TCTTCACACT
    GACACAGAAA AAGAGGCCAT CTCTCCAGGG AGGTCAGTGG AACCGAGTGC TGAAGAGAAA
    AGTTTAATCA GTGATAAAAC AGCCGAATGG ATTGCAGAGG AGGAGGATGA CGTGTTTGTG
    GCTTCCCGCA CAACTGAAGA TTTATTTACT GTGATACACA GGTCCAAAAG AAAGCTACTG
    GGCTGGAAAG AGACTGGGGA GGGCTTCACG GGAAGCAAAC CCAGCTCCCA TTCACCAGTG
    AAGAATACAG CTGATTCTCC CACCGGTGAG GCTGCTGCTG CCCCAGGGCC AAGTAGCAGT
    GCCTGCCTAG ATGCTGGTAG AAATGATGAT TTCAAGGCCT TGCTCCAGAA GAAGGGAAGT
    AAGGCAACTC CCAGGACCCG CCCCTCAGCG GCCGAGCTGC TGAAGACCAC TAACCCTCTG
    GCTCGAAGGA TTATTGCACA GTTCTCAAAA GACTACGAAC CCACTGACAA CCCCAGTACC
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu56080
    Clone ID Related Accession (Same CDS sequence) XM_006527721.3 , XM_006527721.4
    Accession Version XM_006527721.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3408bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-21
    Organism Mus musculus(house mouse)
    Product NHS-like protein 2 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000086.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006527721.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    ATGGCAGCAC AGTCTGGAGC TTGGAATGCT GCAGCTAACT CGGGTCGGGA AAATGCGACA 
    GCGACTGCCC ACTCGAGGTC GTCATGGCGA CAGCCAGTGA ACGTGTTCCT CTCCTCGGGC
    AGGCCCCCGA GTGTAGAGGA ACTGCTTCGC GAGGCGCAGC TCAATCTCCA GAGCCTGTTG
    CAAGAAGAAT ATGAGGAACA GTATTCGGAG GCCAGACTTC TGGGGCAGAC CTTCCGCTCT
    TCTGATGGGG CCCCTGAGCC TACTCCCAGC CCAAGGCCCC AGTCTGCCAA GCGTCTGGAG
    TTTGTACTGA TGCCTGCAAA ACGGCAGCTG AGCGAGGATG AGACCACGAC CCAGGGTGTG
    AGGGCCCCTG AGGCCTGCCT GAGCCTGTCT ACTGCCAACA AGCAAAGTGC CTGGAATGAC
    CCCTTCCCTC TACCCATCCT AAAGGAGAGG CTCTGGCTTC AGCCCTGCTC CACACAGTCT
    GACCTTGTGC CAATCAACAT CTCTGGGCAG CAGTTTGATA GACATGCAAG TTTTCGACAC
    TCGTTGTTTA ACACAGAGAC AGCCGTGAAC CCCAAGTCCA CCCTGAGGCG GAGGCGGACC
    ATTATTGGAT TCTCTAACTT TTCTCAGCGA GACCAAGGTC ACAGCAGCAG CCCCACAGGC
    AGCCTGGCCC GCTCTGCCAC CTCAGACATC AGGCCTGGCC ACTCAGCTCC ACAAGTTGTT
    CAGGGAAGAG TTGCTGTTGG GCAGGAAGCT CGGTTCCCAA GTCTCACCTC ACCAGGACTG
    AGACACTCTT CTAGTGAGCC CGGAGACGCT CACCAGGCAC GCAGTGCCTC AGACCATCCC
    GGCATGGAGA GCATGGCAGT GGTGTACAGT GTCCCCAGTT CTTGCAATGG ACCAACAGAG
    TCAACATTCT CCACTTCCTG GAAGGGAGAT GCTTTCACAT ACATGACCCC AAGTGCCAGC
    AGCCAGGGCA ATCAAGTCAG TGAAAATGGA AAAAACCCTT CCTCGGGCAA TTCTTGGGTC
    CCTCTGAACA CACTCCCACC TCTGGTTCCT AAGGAGGCGG CCACGCTCTT TGTCACCCGT
    GATAACCCAG CAGGATGCAC TGGGCTACCC AGCTACTCTG AGCACCCCAC TCTACGAAGA
    CAGATACCAG AAAGACCTCC CAAGATTGGC CTTCTTGCCC GTGGTACCTC AAGGCTGGAA
    ACAGGCCCAG GTGGGACCAA CAGGTTCCGG GAGCGGTCAC TGTCTGTACC CACAGACTCG
    GGTGTTACCT CAGTGGACTA TGACGAAGAA CAGAAGACCA GTGAGACCCG CATCCTGCCT
    TATGCCAGTA CAAGCTCCGA GGGCAGTAAC AGTACTGACA ACATTGCAGC CCTCAGCACT
    GAGCAGGAGG CACGGCACAG AAGGCAAAGA TCCAAAAGTA TTTCACTCAA GAAGGCCAAA
    AAGAAGCCCT CTCCACCCAT GCGAAGTGTC TCACTCGTCA AAGATGAGCC AGCCCTCCCA
    CCAGAAGGCG AATTGGTACT GCCCAAGGAC CAGAGGCCTA GGAGCCTTTG CCTCTCCTTG
    GAACACCAAG GGCACCACCC ACCTCACCCA GATGCTCAAG GTCACCCAGC TGTGCCAATG
    CTCAAAGATC CAGGAAGTAC GCAGTTCTCT CACCACTGGT ATCTTACTGA CTGGAAGTCT
    GGTGACACCT ACCAGTCCTT GTCCAGCTCC AGCACTGCCA CCGGCACCAC GGTCATTGAG
    TGCACTCAAG TTCAGGGAAG CTCAGAGTCT CTGGCCTCCC CTTCCACCTC CAGAGCGACA
    ACACCCTCCC AGCTCTCCAT TGAGGTGGAA GCCAGGGAAG TAGCCTCTCC TGGGAGGCCC
    ACTGGACTGA TGTCCCCTTC CAGTGGTTAC TCCAGCCAGT CAGAGACACC CACACCCACT
    GTCTCCATGT CCTTGACCCT GGGCCACTTA CCTCCTCCAA GTGCTAGTGT CAGAGTACGT
    CCAGTGGTAC CAGAAAGGAA GTCATCACTG CCCCCAACAT CACCAATGGA GAAAATCTGC
    AAGTCACGGT TATCATTTGA CCTACCACTG ACCTCTTCAA CCACCCTGGA TCTGTCGGGG
    ATGAGTATCT CCATCCGCAG CAAAACCAAG GTGAGCCGCC ATCACTCTGA TACCAATTTT
    GGGGTCAAAC TGGCACAGAA AACTAGTCCA AACCAACCGA TCATGCCCAT GGTTACTCAG
    TCTGACCTCC GTTCTGTTCG CCTGAGGTCA GTCAGCAAGT CTGAGCCAGA AGACGACATT
    GAGAGTCCAG ATTACATAGA GGAACCTGGA GCAGAGGAAG TCTTCACCAT GCCAGAGAGA
    AAAGTGAAAC CTCCCATAGC CGAGAAACCT CCATTGGCCC GAAGGCCTCC AAGTTTGGTC
    CACAGGCCGC CCTCTCTCCC TGGGGAGTAT CCACTAACTT CTCCTACTAT GGCTATGGCA
    TCCAGGAGCT CCATTCCACA CATGAAGCAG CTTCCCCAAG ACAGCTACAC AGTGTTGCGG
    AAACCAAAGT CACCCAGCTT CCCCGGGGAG TCAACAGCAT CCTCCAGCCT TGTCCTCTCA
    CCTCTTGCCA GTTCCTCTGG TGCTTTCTTC TCAGGAACAC AGCAACCTCC CCAGGCCAGT
    GTAGAAGACG GGGGCCCCAA GGTGAGAGCC CTGCCTGAAA GAATCGGCCT CCAGAGCCAG
    GAAGAAGCTG AGAAAAAGAT GACCAAGATT CCACCTCCGG TACCAAAAAA GCCCAGTGTG
    CTCTACCTGC CTCTCACCTC CCCTGTAGCT CAAATGGATG CCTGCATGGC AGAACCAAGG
    CTACCTTTCA GCCCCATCAT CACCCTGGAG GAAGATGGCA AGTGTCCCTC CACTGGTGAT
    GACCAGAAGT CACCTGGTAA AGGGGTGACT TCGCCTCTTC ACACTGACAC AGAAAAAGAG
    GCCATCTCTC CAGGGAGGTC AGTGGAACCG AGTGCTGAAG AGAAAAGTTT AATCAGTGAT
    AAAACAGCCG AATGGATTGC AGAGGAGGAG GATGACGTGT TTGTGGCTTC CCGCACAACT
    GAAGATTTAT TTACTGTGAT ACACAGGTCC AAAAGAAAGC TACTGGGCTG GAAAGAGACT
    GGGGAGGGCT TCACGGGAAG CAAACCCAGC TCCCATTCAC CAGTGAAGAA TACAGCTGAT
    TCTCCCACCG GTGAGGCTGC TGCTGCCCCA GGGCCAAGTA GCAGTGCCTG CCTAGATGCT
    GGTAGAAATG ATGATTTCAA GGCCTTGCTC CAGAAGAAGG GAAGTAAGGC AACTCCCAGG
    ACCCGCCCCT CAGCGGCCGA GCTGCTGAAG ACCACTAACC CTCTGGCTCG AAGGATTATT
    GCACAGTTCT CAAAAGACTA CGAACCCACT GACAACCCCA GTACCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006527784.1
    CDS3615..7022
    Translation

    Target ORF information:

    RefSeq Version XM_006527721.3
    Organism Mus musculus(house mouse)
    Definition Mus musculus NHS-like 2 (Nhsl2), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006527721.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    ATGGCAGCAC AGTCTGGAGC TTGGAATGCT GCAGCTAACT CGGGTCGGGA AAATGCGACA 
    GCGACTGCCC ACTCGAGGTC GTCATGGCGA CAGCCAGTGA ACGTGTTCCT CTCCTCGGGC
    AGGCCCCCGA GTGTAGAGGA ACTGCTTCGC GAGGCGCAGC TCAATCTCCA GAGCCTGTTG
    CAAGAAGAAT ATGAGGAACA GTATTCGGAG GCCAGACTTC TGGGGCAGAC CTTCCGCTCT
    TCTGATGGGG CCCCTGAGCC TACTCCCAGC CCAAGGCCCC AGTCTGCCAA GCGTCTGGAG
    TTTGTACTGA TGCCTGCAAA ACGGCAGCTG AGCGAGGATG AGACCACGAC CCAGGGTGTG
    AGGGCCCCTG AGGCCTGCCT GAGCCTGTCT ACTGCCAACA AGCAAAGTGC CTGGAATGAC
    CCCTTCCCTC TACCCATCCT AAAGGAGAGG CTCTGGCTTC AGCCCTGCTC CACACAGTCT
    GACCTTGTGC CAATCAACAT CTCTGGGCAG CAGTTTGATA GACATGCAAG TTTTCGACAC
    TCGTTGTTTA ACACAGAGAC AGCCGTGAAC CCCAAGTCCA CCCTGAGGCG GAGGCGGACC
    ATTATTGGAT TCTCTAACTT TTCTCAGCGA GACCAAGGTC ACAGCAGCAG CCCCACAGGC
    AGCCTGGCCC GCTCTGCCAC CTCAGACATC AGGCCTGGCC ACTCAGCTCC ACAAGTTGTT
    CAGGGAAGAG TTGCTGTTGG GCAGGAAGCT CGGTTCCCAA GTCTCACCTC ACCAGGACTG
    AGACACTCTT CTAGTGAGCC CGGAGACGCT CACCAGGCAC GCAGTGCCTC AGACCATCCC
    GGCATGGAGA GCATGGCAGT GGTGTACAGT GTCCCCAGTT CTTGCAATGG ACCAACAGAG
    TCAACATTCT CCACTTCCTG GAAGGGAGAT GCTTTCACAT ACATGACCCC AAGTGCCAGC
    AGCCAGGGCA ATCAAGTCAG TGAAAATGGA AAAAACCCTT CCTCGGGCAA TTCTTGGGTC
    CCTCTGAACA CACTCCCACC TCTGGTTCCT AAGGAGGCGG CCACGCTCTT TGTCACCCGT
    GATAACCCAG CAGGATGCAC TGGGCTACCC AGCTACTCTG AGCACCCCAC TCTACGAAGA
    CAGATACCAG AAAGACCTCC CAAGATTGGC CTTCTTGCCC GTGGTACCTC AAGGCTGGAA
    ACAGGCCCAG GTGGGACCAA CAGGTTCCGG GAGCGGTCAC TGTCTGTACC CACAGACTCG
    GGTGTTACCT CAGTGGACTA TGACGAAGAA CAGAAGACCA GTGAGACCCG CATCCTGCCT
    TATGCCAGTA CAAGCTCCGA GGGCAGTAAC AGTACTGACA ACATTGCAGC CCTCAGCACT
    GAGCAGGAGG CACGGCACAG AAGGCAAAGA TCCAAAAGTA TTTCACTCAA GAAGGCCAAA
    AAGAAGCCCT CTCCACCCAT GCGAAGTGTC TCACTCGTCA AAGATGAGCC AGCCCTCCCA
    CCAGAAGGCG AATTGGTACT GCCCAAGGAC CAGAGGCCTA GGAGCCTTTG CCTCTCCTTG
    GAACACCAAG GGCACCACCC ACCTCACCCA GATGCTCAAG GTCACCCAGC TGTGCCAATG
    CTCAAAGATC CAGGAAGTAC GCAGTTCTCT CACCACTGGT ATCTTACTGA CTGGAAGTCT
    GGTGACACCT ACCAGTCCTT GTCCAGCTCC AGCACTGCCA CCGGCACCAC GGTCATTGAG
    TGCACTCAAG TTCAGGGAAG CTCAGAGTCT CTGGCCTCCC CTTCCACCTC CAGAGCGACA
    ACACCCTCCC AGCTCTCCAT TGAGGTGGAA GCCAGGGAAG TAGCCTCTCC TGGGAGGCCC
    ACTGGACTGA TGTCCCCTTC CAGTGGTTAC TCCAGCCAGT CAGAGACACC CACACCCACT
    GTCTCCATGT CCTTGACCCT GGGCCACTTA CCTCCTCCAA GTGCTAGTGT CAGAGTACGT
    CCAGTGGTAC CAGAAAGGAA GTCATCACTG CCCCCAACAT CACCAATGGA GAAAATCTGC
    AAGTCACGGT TATCATTTGA CCTACCACTG ACCTCTTCAA CCACCCTGGA TCTGTCGGGG
    ATGAGTATCT CCATCCGCAG CAAAACCAAG GTGAGCCGCC ATCACTCTGA TACCAATTTT
    GGGGTCAAAC TGGCACAGAA AACTAGTCCA AACCAACCGA TCATGCCCAT GGTTACTCAG
    TCTGACCTCC GTTCTGTTCG CCTGAGGTCA GTCAGCAAGT CTGAGCCAGA AGACGACATT
    GAGAGTCCAG ATTACATAGA GGAACCTGGA GCAGAGGAAG TCTTCACCAT GCCAGAGAGA
    AAAGTGAAAC CTCCCATAGC CGAGAAACCT CCATTGGCCC GAAGGCCTCC AAGTTTGGTC
    CACAGGCCGC CCTCTCTCCC TGGGGAGTAT CCACTAACTT CTCCTACTAT GGCTATGGCA
    TCCAGGAGCT CCATTCCACA CATGAAGCAG CTTCCCCAAG ACAGCTACAC AGTGTTGCGG
    AAACCAAAGT CACCCAGCTT CCCCGGGGAG TCAACAGCAT CCTCCAGCCT TGTCCTCTCA
    CCTCTTGCCA GTTCCTCTGG TGCTTTCTTC TCAGGAACAC AGCAACCTCC CCAGGCCAGT
    GTAGAAGACG GGGGCCCCAA GGTGAGAGCC CTGCCTGAAA GAATCGGCCT CCAGAGCCAG
    GAAGAAGCTG AGAAAAAGAT GACCAAGATT CCACCTCCGG TACCAAAAAA GCCCAGTGTG
    CTCTACCTGC CTCTCACCTC CCCTGTAGCT CAAATGGATG CCTGCATGGC AGAACCAAGG
    CTACCTTTCA GCCCCATCAT CACCCTGGAG GAAGATGGCA AGTGTCCCTC CACTGGTGAT
    GACCAGAAGT CACCTGGTAA AGGGGTGACT TCGCCTCTTC ACACTGACAC AGAAAAAGAG
    GCCATCTCTC CAGGGAGGTC AGTGGAACCG AGTGCTGAAG AGAAAAGTTT AATCAGTGAT
    AAAACAGCCG AATGGATTGC AGAGGAGGAG GATGACGTGT TTGTGGCTTC CCGCACAACT
    GAAGATTTAT TTACTGTGAT ACACAGGTCC AAAAGAAAGC TACTGGGCTG GAAAGAGACT
    GGGGAGGGCT TCACGGGAAG CAAACCCAGC TCCCATTCAC CAGTGAAGAA TACAGCTGAT
    TCTCCCACCG GTGAGGCTGC TGCTGCCCCA GGGCCAAGTA GCAGTGCCTG CCTAGATGCT
    GGTAGAAATG ATGATTTCAA GGCCTTGCTC CAGAAGAAGG GAAGTAAGGC AACTCCCAGG
    ACCCGCCCCT CAGCGGCCGA GCTGCTGAAG ACCACTAACC CTCTGGCTCG AAGGATTATT
    GCACAGTTCT CAAAAGACTA CGAACCCACT GACAACCCCA GTACCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu56082
    Clone ID Related Accession (Same CDS sequence) XM_006527723.4 , XM_006527723.3
    Accession Version XM_006527723.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3099bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-21
    Organism Mus musculus(house mouse)
    Product NHS-like protein 2 isoform X9
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000086.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006527723.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    ATGCCTGCAA AACGGCAGCT GAGCGAGGAT GAGACCACGA CCCAGGGTGT GAGGGCCCCT 
    GAGGCCTGCC TGAGCCTGTC TACTGCCAAC AAGCAAAGTG CCTGGAATGA CCCCTTCCCT
    CTACCCATCC TAAAGGAGAG GCTCTGGCTT CAGCCCTGCT CCACACAGTC TGACCTTGTG
    CCAATCAACA TCTCTGGGCA GCAGTTTGAT AGACATGCAA GTTTTCGACA CTCGTTGTTT
    AACACAGAGA CAGCCGTGAA CCCCAAGTCC ACCCTGAGGC GGAGGCGGAC CATTATTGGA
    TTCTCTAACT TTTCTCAGCG AGACCAAGGT CACAGCAGCA GCCCCACAGG CAGCCTGGCC
    CGCTCTGCCA CCTCAGACAT CAGGCCTGGC CACTCAGCTC CACAAGTTGT TCAGGGAAGA
    GTTGCTGTTG GGCAGGAAGC TCGGTTCCCA AGTCTCACCT CACCAGGACT GAGACACTCT
    TCTAGTGAGC CCGGAGACGC TCACCAGGCA CGCAGTGCCT CAGACCATCC CGGCATGGAG
    AGCATGGCAG TGGTGTACAG TGTCCCCAGT TCTTGCAATG GACCAACAGA GTCAACATTC
    TCCACTTCCT GGAAGGGAGA TGCTTTCACA TACATGACCC CAAGTGCCAG CAGCCAGGGC
    AATCAAGTCA GTGAAAATGG AAAAAACCCT TCCTCGGGCA ATTCTTGGGT CCCTCTGAAC
    ACACTCCCAC CTCTGGTTCC TAAGGAGGCG GCCACGCTCT TTGTCACCCG TGATAACCCA
    GCAGGATGCA CTGGGCTACC CAGCTACTCT GAGCACCCCA CTCTACGAAG ACAGATACCA
    GAAAGACCTC CCAAGATTGG CCTTCTTGCC CGTGGTACCT CAAGGCTGGA AACAGGCCCA
    GGTGGGACCA ACAGGTTCCG GGAGCGGTCA CTGTCTGTAC CCACAGACTC GGGTGTTACC
    TCAGTGGACT ATGACGAAGA ACAGAAGACC AGTGAGACCC GCATCCTGCC TTATGCCAGT
    ACAAGCTCCG AGGGCAGTAA CAGTACTGAC AACATTGCAG CCCTCAGCAC TGAGCAGGAG
    GCACGGCACA GAAGGCAAAG ATCCAAAAGT ATTTCACTCA AGAAGGCCAA AAAGAAGCCC
    TCTCCACCCA TGCGAAGTGT CTCACTCGTC AAAGATGAGC CAGCCCTCCC ACCAGAAGGC
    GAATTGGTAC TGCCCAAGGA CCAGAGGCCT AGGAGCCTTT GCCTCTCCTT GGAACACCAA
    GGGCACCACC CACCTCACCC AGATGCTCAA GGTCACCCAG CTGTGCCAAT GCTCAAAGAT
    CCAGGAAGTA CGCAGTTCTC TCACCACTGG TATCTTACTG ACTGGAAGTC TGGTGACACC
    TACCAGTCCT TGTCCAGCTC CAGCACTGCC ACCGGCACCA CGGTCATTGA GTGCACTCAA
    GTTCAGGGAA GCTCAGAGTC TCTGGCCTCC CCTTCCACCT CCAGAGCGAC AACACCCTCC
    CAGCTCTCCA TTGAGGTGGA AGCCAGGGAA GTAGCCTCTC CTGGGAGGCC CACTGGACTG
    ATGTCCCCTT CCAGTGGTTA CTCCAGCCAG TCAGAGACAC CCACACCCAC TGTCTCCATG
    TCCTTGACCC TGGGCCACTT ACCTCCTCCA AGTGCTAGTG TCAGAGTACG TCCAGTGGTA
    CCAGAAAGGA AGTCATCACT GCCCCCAACA TCACCAATGG AGAAAATCTG CAAGTCACGG
    TTATCATTTG ACCTACCACT GACCTCTTCA ACCACCCTGG ATCTGTCGGG GATGAGTATC
    TCCATCCGCA GCAAAACCAA GGTGAGCCGC CATCACTCTG ATACCAATTT TGGGGTCAAA
    CTGGCACAGA AAACTAGTCC AAACCAACCG ATCATGCCCA TGGTTACTCA GTCTGACCTC
    CGTTCTGTTC GCCTGAGGTC AGTCAGCAAG TCTGAGCCAG AAGACGACAT TGAGAGTCCA
    GATTACATAG AGGAACCTGG AGCAGAGGAA GTCTTCACCA TGCCAGAGAG AAAAGTGAAA
    CCTCCCATAG CCGAGAAACC TCCATTGGCC CGAAGGCCTC CAAGTTTGGT CCACAGGCCG
    CCCTCTCTCC CTGGGGAGTA TCCACTAACT TCTCCTACTA TGGCTATGGC ATCCAGGAGC
    TCCATTCCAC ACATGAAGCA GCTTCCCCAA GACAGCTACA CAGTGTTGCG GAAACCAAAG
    TCACCCAGCT TCCCCGGGGA GTCAACAGCA TCCTCCAGCC TTGTCCTCTC ACCTCTTGCC
    AGTTCCTCTG GTGCTTTCTT CTCAGGAACA CAGCAACCTC CCCAGGCCAG TGTAGAAGAC
    GGGGGCCCCA AGGTGAGAGC CCTGCCTGAA AGAATCGGCC TCCAGAGCCA GGAAGAAGCT
    GAGAAAAAGA TGACCAAGAT TCCACCTCCG GTACCAAAAA AGCCCAGTGT GCTCTACCTG
    CCTCTCACCT CCCCTGTAGC TCAAATGGAT GCCTGCATGG CAGAACCAAG GCTACCTTTC
    AGCCCCATCA TCACCCTGGA GGAAGATGGC AAGTGTCCCT CCACTGGTGA TGACCAGAAG
    TCACCTGGTA AAGGGGTGAC TTCGCCTCTT CACACTGACA CAGAAAAAGA GGCCATCTCT
    CCAGGGAGGT CAGTGGAACC GAGTGCTGAA GAGAAAAGTT TAATCAGTGA TAAAACAGCC
    GAATGGATTG CAGAGGAGGA GGATGACGTG TTTGTGGCTT CCCGCACAAC TGAAGATTTA
    TTTACTGTGA TACACAGGTC CAAAAGAAAG CTACTGGGCT GGAAAGAGAC TGGGGAGGGC
    TTCACGGGAA GCAAACCCAG CTCCCATTCA CCAGTGAAGA ATACAGCTGA TTCTCCCACC
    GGTGAGGCTG CTGCTGCCCC AGGGCCAAGT AGCAGTGCCT GCCTAGATGC TGGTAGAAAT
    GATGATTTCA AGGCCTTGCT CCAGAAGAAG GGAAGTAAGG CAACTCCCAG GACCCGCCCC
    TCAGCGGCCG AGCTGCTGAA GACCACTAAC CCTCTGGCTC GAAGGATTAT TGCACAGTTC
    TCAAAAGACT ACGAACCCAC TGACAACCCC AGTACCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006527786.1
    CDS298..3396
    Translation

    Target ORF information:

    RefSeq Version XM_006527723.3
    Organism Mus musculus(house mouse)
    Definition Mus musculus NHS-like 2 (Nhsl2), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006527723.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    ATGCCTGCAA AACGGCAGCT GAGCGAGGAT GAGACCACGA CCCAGGGTGT GAGGGCCCCT 
    GAGGCCTGCC TGAGCCTGTC TACTGCCAAC AAGCAAAGTG CCTGGAATGA CCCCTTCCCT
    CTACCCATCC TAAAGGAGAG GCTCTGGCTT CAGCCCTGCT CCACACAGTC TGACCTTGTG
    CCAATCAACA TCTCTGGGCA GCAGTTTGAT AGACATGCAA GTTTTCGACA CTCGTTGTTT
    AACACAGAGA CAGCCGTGAA CCCCAAGTCC ACCCTGAGGC GGAGGCGGAC CATTATTGGA
    TTCTCTAACT TTTCTCAGCG AGACCAAGGT CACAGCAGCA GCCCCACAGG CAGCCTGGCC
    CGCTCTGCCA CCTCAGACAT CAGGCCTGGC CACTCAGCTC CACAAGTTGT TCAGGGAAGA
    GTTGCTGTTG GGCAGGAAGC TCGGTTCCCA AGTCTCACCT CACCAGGACT GAGACACTCT
    TCTAGTGAGC CCGGAGACGC TCACCAGGCA CGCAGTGCCT CAGACCATCC CGGCATGGAG
    AGCATGGCAG TGGTGTACAG TGTCCCCAGT TCTTGCAATG GACCAACAGA GTCAACATTC
    TCCACTTCCT GGAAGGGAGA TGCTTTCACA TACATGACCC CAAGTGCCAG CAGCCAGGGC
    AATCAAGTCA GTGAAAATGG AAAAAACCCT TCCTCGGGCA ATTCTTGGGT CCCTCTGAAC
    ACACTCCCAC CTCTGGTTCC TAAGGAGGCG GCCACGCTCT TTGTCACCCG TGATAACCCA
    GCAGGATGCA CTGGGCTACC CAGCTACTCT GAGCACCCCA CTCTACGAAG ACAGATACCA
    GAAAGACCTC CCAAGATTGG CCTTCTTGCC CGTGGTACCT CAAGGCTGGA AACAGGCCCA
    GGTGGGACCA ACAGGTTCCG GGAGCGGTCA CTGTCTGTAC CCACAGACTC GGGTGTTACC
    TCAGTGGACT ATGACGAAGA ACAGAAGACC AGTGAGACCC GCATCCTGCC TTATGCCAGT
    ACAAGCTCCG AGGGCAGTAA CAGTACTGAC AACATTGCAG CCCTCAGCAC TGAGCAGGAG
    GCACGGCACA GAAGGCAAAG ATCCAAAAGT ATTTCACTCA AGAAGGCCAA AAAGAAGCCC
    TCTCCACCCA TGCGAAGTGT CTCACTCGTC AAAGATGAGC CAGCCCTCCC ACCAGAAGGC
    GAATTGGTAC TGCCCAAGGA CCAGAGGCCT AGGAGCCTTT GCCTCTCCTT GGAACACCAA
    GGGCACCACC CACCTCACCC AGATGCTCAA GGTCACCCAG CTGTGCCAAT GCTCAAAGAT
    CCAGGAAGTA CGCAGTTCTC TCACCACTGG TATCTTACTG ACTGGAAGTC TGGTGACACC
    TACCAGTCCT TGTCCAGCTC CAGCACTGCC ACCGGCACCA CGGTCATTGA GTGCACTCAA
    GTTCAGGGAA GCTCAGAGTC TCTGGCCTCC CCTTCCACCT CCAGAGCGAC AACACCCTCC
    CAGCTCTCCA TTGAGGTGGA AGCCAGGGAA GTAGCCTCTC CTGGGAGGCC CACTGGACTG
    ATGTCCCCTT CCAGTGGTTA CTCCAGCCAG TCAGAGACAC CCACACCCAC TGTCTCCATG
    TCCTTGACCC TGGGCCACTT ACCTCCTCCA AGTGCTAGTG TCAGAGTACG TCCAGTGGTA
    CCAGAAAGGA AGTCATCACT GCCCCCAACA TCACCAATGG AGAAAATCTG CAAGTCACGG
    TTATCATTTG ACCTACCACT GACCTCTTCA ACCACCCTGG ATCTGTCGGG GATGAGTATC
    TCCATCCGCA GCAAAACCAA GGTGAGCCGC CATCACTCTG ATACCAATTT TGGGGTCAAA
    CTGGCACAGA AAACTAGTCC AAACCAACCG ATCATGCCCA TGGTTACTCA GTCTGACCTC
    CGTTCTGTTC GCCTGAGGTC AGTCAGCAAG TCTGAGCCAG AAGACGACAT TGAGAGTCCA
    GATTACATAG AGGAACCTGG AGCAGAGGAA GTCTTCACCA TGCCAGAGAG AAAAGTGAAA
    CCTCCCATAG CCGAGAAACC TCCATTGGCC CGAAGGCCTC CAAGTTTGGT CCACAGGCCG
    CCCTCTCTCC CTGGGGAGTA TCCACTAACT TCTCCTACTA TGGCTATGGC ATCCAGGAGC
    TCCATTCCAC ACATGAAGCA GCTTCCCCAA GACAGCTACA CAGTGTTGCG GAAACCAAAG
    TCACCCAGCT TCCCCGGGGA GTCAACAGCA TCCTCCAGCC TTGTCCTCTC ACCTCTTGCC
    AGTTCCTCTG GTGCTTTCTT CTCAGGAACA CAGCAACCTC CCCAGGCCAG TGTAGAAGAC
    GGGGGCCCCA AGGTGAGAGC CCTGCCTGAA AGAATCGGCC TCCAGAGCCA GGAAGAAGCT
    GAGAAAAAGA TGACCAAGAT TCCACCTCCG GTACCAAAAA AGCCCAGTGT GCTCTACCTG
    CCTCTCACCT CCCCTGTAGC TCAAATGGAT GCCTGCATGG CAGAACCAAG GCTACCTTTC
    AGCCCCATCA TCACCCTGGA GGAAGATGGC AAGTGTCCCT CCACTGGTGA TGACCAGAAG
    TCACCTGGTA AAGGGGTGAC TTCGCCTCTT CACACTGACA CAGAAAAAGA GGCCATCTCT
    CCAGGGAGGT CAGTGGAACC GAGTGCTGAA GAGAAAAGTT TAATCAGTGA TAAAACAGCC
    GAATGGATTG CAGAGGAGGA GGATGACGTG TTTGTGGCTT CCCGCACAAC TGAAGATTTA
    TTTACTGTGA TACACAGGTC CAAAAGAAAG CTACTGGGCT GGAAAGAGAC TGGGGAGGGC
    TTCACGGGAA GCAAACCCAG CTCCCATTCA CCAGTGAAGA ATACAGCTGA TTCTCCCACC
    GGTGAGGCTG CTGCTGCCCC AGGGCCAAGT AGCAGTGCCT GCCTAGATGC TGGTAGAAAT
    GATGATTTCA AGGCCTTGCT CCAGAAGAAG GGAAGTAAGG CAACTCCCAG GACCCGCCCC
    TCAGCGGCCG AGCTGCTGAA GACCACTAAC CCTCTGGCTC GAAGGATTAT TGCACAGTTC
    TCAAAAGACT ACGAACCCAC TGACAACCCC AGTACCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu75773
    Clone ID Related Accession (Same CDS sequence) XM_011247499.2 , XM_011247499.1
    Accession Version XM_011247499.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3729bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-21
    Organism Mus musculus(house mouse)
    Product NHS-like protein 2 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000086.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGCCGTTCT ACCGGCGCAC TGTGGTGCCC CAGCGTCTGT GCCCGCGCAA CCCTCCGCAG 
    CCGCTGGCTG AGCTCCGCGA CGTGAGCCAC CTGGCCGCGC TCAGTCTGCT CCGCCAGCTC
    GCAGACCTCT GCGGCCACTC GCTGGCTCTG CTGGAGGACC TCGAGGGGCA CCTGCTGGCT
    CTGGGGCGCC GCACCGACAG CCTGTACCGG CGCACCGTCC GGCTCCGCCG CCGCCTTCCC
    TGTCGCCTGC TTGGCCCGGA GGATGATGAA GAATTGCTAG GTGCCTGCCA CTGGGTGAAC
    GGGACTCGCA TCCTGAGGGC AAGGGAGCTT GCTGATGTAG CCAGCACAGC TGCAGCTAAC
    TCGGGTCGGG AAAATGCGAC AGCGACTGCC CACTCGAGGT CGTCATGGCG ACAGCCAGTG
    AACGTGTTCC TCTCCTCGGG CAGGCCCCCG AGTGTAGAGG AACTGCTTCG CGAGGCGCAG
    CTCAATCTCC AGAGCCTGTT GCAAGAAGAA TATGAGGAAC AGTATTCGGA GGCCAGACTT
    CTGGGGCAGA CCTTCCGCTC TTCTGATGGG GCCCCTGAGC CTACTCCCAG CCCAAGGCCC
    CAGTCTGCCA AGCGTCTGGA GTTTGTACTG ATGCCTGCAA AACGGCAGCT GAGCGAGGAT
    GAGACCACGA CCCAGGGTGT GAGGGCCCCT GAGGCCTGCC TGAGCCTGTC TACTGCCAAC
    AAGCAAAGTG CCTGGAATGA CCCCTTCCCT CTACCCATCC TAAAGGAGAG GCTCTGGCTT
    CAGCCCTGCT CCACACAGTC TGACCTTGTG CCAATCAACA TCTCTGGGCA GCAGTTTGAT
    AGACATGCAA GTTTTCGACA CTCGTTGTTT AACACAGAGA CAGCCGTGAA CCCCAAGTCC
    ACCCTGAGGC GGAGGCGGAC CATTATTGGA TTCTCTAACT TTTCTCAGCG AGACCAAGGT
    CACAGCAGCA GCCCCACAGG CAGCCTGGCC CGCTCTGCCA CCTCAGACAT CAGGCCTGGC
    CACTCAGCTC CACAAGTTGT TCAGGGAAGA GTTGCTGTTG GGCAGGAAGC TCGGTTCCCA
    AGTCTCACCT CACCAGGACT GAGACACTCT TCTAGTGAGC CCGGAGACGC TCACCAGGCA
    CGCAGTGCCT CAGACCATCC CGGCATGGAG AGCATGGCAG TGGTGTACAG TGTCCCCAGT
    TCTTGCAATG GACCAACAGA GTCAACATTC TCCACTTCCT GGAAGGGAGA TGCTTTCACA
    TACATGACCC CAAGTGCCAG CAGCCAGGGC AATCAAGTCA GTGAAAATGG AAAAAACCCT
    TCCTCGGGCA ATTCTTGGGT CCCTCTGAAC ACACTCCCAC CTCTGGTTCC TAAGGAGGCG
    GCCACGCTCT TTGTCACCCG TGATAACCCA GCAGGATGCA CTGGGCTACC CAGCTACTCT
    GAGCACCCCA CTCTACGAAG ACAGATACCA GAAAGACCTC CCAAGATTGG CCTTCTTGCC
    CGTGGTACCT CAAGGCTGGA AACAGGCCCA GGTGGGACCA ACAGGTTCCG GGAGCGGTCA
    CTGTCTGTAC CCACAGACTC GGGTGTTACC TCAGTGGACT ATGACGAAGA ACAGAAGACC
    AGTGAGACCC GCATCCTGCC TTATGCCAGT ACAAGCTCCG AGGGCAGTAA CAGTACTGAC
    AACATTGCAG CCCTCAGCAC TGAGCAGGAG GCACGGCACA GAAGGCAAAG ATCCAAAAGT
    ATTTCACTCA AGAAGGCCAA AAAGAAGCCC TCTCCACCCA TGCGAAGTGT CTCACTCGTC
    AAAGATGAGC CAGCCCTCCC ACCAGAAGGC GAATTGGTAC TGCCCAAGGA CCAGAGGCCT
    AGGAGCCTTT GCCTCTCCTT GGAACACCAA GGGCACCACC CACCTCACCC AGATGCTCAA
    GGTCACCCAG CTGTGCCAAT GCTCAAAGAT CCAGGAAGTA CGCAGTTCTC TCACCACTGG
    TATCTTACTG ACTGGAAGTC TGGTGACACC TACCAGTCCT TGTCCAGCTC CAGCACTGCC
    ACCGGCACCA CGGTCATTGA GTGCACTCAA GTTCAGGGAA GCTCAGAGTC TCTGGCCTCC
    CCTTCCACCT CCAGAGCGAC AACACCCTCC CAGCTCTCCA TTGAGGTGGA AGCCAGGGAA
    GTAGCCTCTC CTGGGAGGCC CACTGGACTG ATGTCCCCTT CCAGTGGTTA CTCCAGCCAG
    TCAGAGACAC CCACACCCAC TGTCTCCATG TCCTTGACCC TGGGCCACTT ACCTCCTCCA
    AGTGCTAGTG TCAGAGTACG TCCAGTGGTA CCAGAAAGGA AGTCATCACT GCCCCCAACA
    TCACCAATGG AGAAAATCTG CAAGTCACGG TTATCATTTG ACCTACCACT GACCTCTTCA
    ACCACCCTGG ATCTGTCGGG GATGAGTATC TCCATCCGCA GCAAAACCAA GGTGAGCCGC
    CATCACTCTG ATACCAATTT TGGGGTCAAA CTGGCACAGA AAACTAGTCC AAACCAACCG
    ATCATGCCCA TGGTTACTCA GTCTGACCTC CGTTCTGTTC GCCTGAGGTC AGTCAGCAAG
    TCTGAGCCAG AAGACGACAT TGAGAGTCCA GATTACATAG AGGAACCTGG AGCAGAGGAA
    GTCTTCACCA TGCCAGAGAG AAAAGTGAAA CCTCCCATAG CCGAGAAACC TCCATTGGCC
    CGAAGGCCTC CAAGTTTGGT CCACAGGCCG CCCTCTCTCC CTGGGGAGTA TCCACTAACT
    TCTCCTACTA TGGCTATGGC ATCCAGGAGC TCCATTCCAC ACATGAAGCA GCTTCCCCAA
    GACAGCTACA CAGTGTTGCG GAAACCAAAG TCACCCAGCT TCCCCGGGGA GTCAACAGCA
    TCCTCCAGCC TTGTCCTCTC ACCTCTTGCC AGTTCCTCTG GTGCTTTCTT CTCAGGAACA
    CAGCAACCTC CCCAGGCCAG TGTAGAAGAC GGGGGCCCCA AGGTGAGAGC CCTGCCTGAA
    AGAATCGGCC TCCAGAGCCA GGAAGAAGCT GAGAAAAAGA TGACCAAGAT TCCACCTCCG
    GTACCAAAAA AGCCCAGTGT GCTCTACCTG CCTCTCACCT CCCCTGTAGC TCAAATGGAT
    GCCTGCATGG CAGAACCAAG GCTACCTTTC AGCCCCATCA TCACCCTGGA GGAAGATGGC
    AAGTGTCCCT CCACTGGTGA TGACCAGAAG TCACCTGGTA AAGGGGTGAC TTCGCCTCTT
    CACACTGACA CAGAAAAAGA GGCCATCTCT CCAGGGAGGT CAGTGGAACC GAGTGCTGAA
    GAGAAAAGTT TAATCAGTGA TAAAACAGCC GAATGGATTG CAGAGGAGGA GGATGACGTG
    TTTGTGGCTT CCCGCACAAC TGAAGATTTA TTTACTGTGA TACACAGGTC CAAAAGAAAG
    CTACTGGGCT GGAAAGAGAC TGGGGAGGGC TTCACGGGAA GCAAACCCAG CTCCCATTCA
    CCAGTGAAGA ATACAGCTGA TTCTCCCACC GGTGAGGCTG CTGCTGCCCC AGGGCCAAGT
    AGCAGTGCCT GCCTAGATGC TGGTAGAAAT GATGATTTCA AGGCCTTGCT CCAGAAGAAG
    GGAAGTAAGG CAACTCCCAG GACCCGCCCC TCAGCGGCCG AGCTGCTGAA GACCACTAAC
    CCTCTGGCTC GAAGGATTAT TGCACAGTTC TCAAAAGACT ACGAACCCAC TGACAACCCC
    AGTACCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011245801.1
    CDS900..4628
    Translation

    Target ORF information:

    RefSeq Version XM_011247499.1
    Organism Mus musculus(house mouse)
    Definition Mus musculus NHS-like 2 (Nhsl2), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011247499.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGCCGTTCT ACCGGCGCAC TGTGGTGCCC CAGCGTCTGT GCCCGCGCAA CCCTCCGCAG 
    CCGCTGGCTG AGCTCCGCGA CGTGAGCCAC CTGGCCGCGC TCAGTCTGCT CCGCCAGCTC
    GCAGACCTCT GCGGCCACTC GCTGGCTCTG CTGGAGGACC TCGAGGGGCA CCTGCTGGCT
    CTGGGGCGCC GCACCGACAG CCTGTACCGG CGCACCGTCC GGCTCCGCCG CCGCCTTCCC
    TGTCGCCTGC TTGGCCCGGA GGATGATGAA GAATTGCTAG GTGCCTGCCA CTGGGTGAAC
    GGGACTCGCA TCCTGAGGGC AAGGGAGCTT GCTGATGTAG CCAGCACAGC TGCAGCTAAC
    TCGGGTCGGG AAAATGCGAC AGCGACTGCC CACTCGAGGT CGTCATGGCG ACAGCCAGTG
    AACGTGTTCC TCTCCTCGGG CAGGCCCCCG AGTGTAGAGG AACTGCTTCG CGAGGCGCAG
    CTCAATCTCC AGAGCCTGTT GCAAGAAGAA TATGAGGAAC AGTATTCGGA GGCCAGACTT
    CTGGGGCAGA CCTTCCGCTC TTCTGATGGG GCCCCTGAGC CTACTCCCAG CCCAAGGCCC
    CAGTCTGCCA AGCGTCTGGA GTTTGTACTG ATGCCTGCAA AACGGCAGCT GAGCGAGGAT
    GAGACCACGA CCCAGGGTGT GAGGGCCCCT GAGGCCTGCC TGAGCCTGTC TACTGCCAAC
    AAGCAAAGTG CCTGGAATGA CCCCTTCCCT CTACCCATCC TAAAGGAGAG GCTCTGGCTT
    CAGCCCTGCT CCACACAGTC TGACCTTGTG CCAATCAACA TCTCTGGGCA GCAGTTTGAT
    AGACATGCAA GTTTTCGACA CTCGTTGTTT AACACAGAGA CAGCCGTGAA CCCCAAGTCC
    ACCCTGAGGC GGAGGCGGAC CATTATTGGA TTCTCTAACT TTTCTCAGCG AGACCAAGGT
    CACAGCAGCA GCCCCACAGG CAGCCTGGCC CGCTCTGCCA CCTCAGACAT CAGGCCTGGC
    CACTCAGCTC CACAAGTTGT TCAGGGAAGA GTTGCTGTTG GGCAGGAAGC TCGGTTCCCA
    AGTCTCACCT CACCAGGACT GAGACACTCT TCTAGTGAGC CCGGAGACGC TCACCAGGCA
    CGCAGTGCCT CAGACCATCC CGGCATGGAG AGCATGGCAG TGGTGTACAG TGTCCCCAGT
    TCTTGCAATG GACCAACAGA GTCAACATTC TCCACTTCCT GGAAGGGAGA TGCTTTCACA
    TACATGACCC CAAGTGCCAG CAGCCAGGGC AATCAAGTCA GTGAAAATGG AAAAAACCCT
    TCCTCGGGCA ATTCTTGGGT CCCTCTGAAC ACACTCCCAC CTCTGGTTCC TAAGGAGGCG
    GCCACGCTCT TTGTCACCCG TGATAACCCA GCAGGATGCA CTGGGCTACC CAGCTACTCT
    GAGCACCCCA CTCTACGAAG ACAGATACCA GAAAGACCTC CCAAGATTGG CCTTCTTGCC
    CGTGGTACCT CAAGGCTGGA AACAGGCCCA GGTGGGACCA ACAGGTTCCG GGAGCGGTCA
    CTGTCTGTAC CCACAGACTC GGGTGTTACC TCAGTGGACT ATGACGAAGA ACAGAAGACC
    AGTGAGACCC GCATCCTGCC TTATGCCAGT ACAAGCTCCG AGGGCAGTAA CAGTACTGAC
    AACATTGCAG CCCTCAGCAC TGAGCAGGAG GCACGGCACA GAAGGCAAAG ATCCAAAAGT
    ATTTCACTCA AGAAGGCCAA AAAGAAGCCC TCTCCACCCA TGCGAAGTGT CTCACTCGTC
    AAAGATGAGC CAGCCCTCCC ACCAGAAGGC GAATTGGTAC TGCCCAAGGA CCAGAGGCCT
    AGGAGCCTTT GCCTCTCCTT GGAACACCAA GGGCACCACC CACCTCACCC AGATGCTCAA
    GGTCACCCAG CTGTGCCAAT GCTCAAAGAT CCAGGAAGTA CGCAGTTCTC TCACCACTGG
    TATCTTACTG ACTGGAAGTC TGGTGACACC TACCAGTCCT TGTCCAGCTC CAGCACTGCC
    ACCGGCACCA CGGTCATTGA GTGCACTCAA GTTCAGGGAA GCTCAGAGTC TCTGGCCTCC
    CCTTCCACCT CCAGAGCGAC AACACCCTCC CAGCTCTCCA TTGAGGTGGA AGCCAGGGAA
    GTAGCCTCTC CTGGGAGGCC CACTGGACTG ATGTCCCCTT CCAGTGGTTA CTCCAGCCAG
    TCAGAGACAC CCACACCCAC TGTCTCCATG TCCTTGACCC TGGGCCACTT ACCTCCTCCA
    AGTGCTAGTG TCAGAGTACG TCCAGTGGTA CCAGAAAGGA AGTCATCACT GCCCCCAACA
    TCACCAATGG AGAAAATCTG CAAGTCACGG TTATCATTTG ACCTACCACT GACCTCTTCA
    ACCACCCTGG ATCTGTCGGG GATGAGTATC TCCATCCGCA GCAAAACCAA GGTGAGCCGC
    CATCACTCTG ATACCAATTT TGGGGTCAAA CTGGCACAGA AAACTAGTCC AAACCAACCG
    ATCATGCCCA TGGTTACTCA GTCTGACCTC CGTTCTGTTC GCCTGAGGTC AGTCAGCAAG
    TCTGAGCCAG AAGACGACAT TGAGAGTCCA GATTACATAG AGGAACCTGG AGCAGAGGAA
    GTCTTCACCA TGCCAGAGAG AAAAGTGAAA CCTCCCATAG CCGAGAAACC TCCATTGGCC
    CGAAGGCCTC CAAGTTTGGT CCACAGGCCG CCCTCTCTCC CTGGGGAGTA TCCACTAACT
    TCTCCTACTA TGGCTATGGC ATCCAGGAGC TCCATTCCAC ACATGAAGCA GCTTCCCCAA
    GACAGCTACA CAGTGTTGCG GAAACCAAAG TCACCCAGCT TCCCCGGGGA GTCAACAGCA
    TCCTCCAGCC TTGTCCTCTC ACCTCTTGCC AGTTCCTCTG GTGCTTTCTT CTCAGGAACA
    CAGCAACCTC CCCAGGCCAG TGTAGAAGAC GGGGGCCCCA AGGTGAGAGC CCTGCCTGAA
    AGAATCGGCC TCCAGAGCCA GGAAGAAGCT GAGAAAAAGA TGACCAAGAT TCCACCTCCG
    GTACCAAAAA AGCCCAGTGT GCTCTACCTG CCTCTCACCT CCCCTGTAGC TCAAATGGAT
    GCCTGCATGG CAGAACCAAG GCTACCTTTC AGCCCCATCA TCACCCTGGA GGAAGATGGC
    AAGTGTCCCT CCACTGGTGA TGACCAGAAG TCACCTGGTA AAGGGGTGAC TTCGCCTCTT
    CACACTGACA CAGAAAAAGA GGCCATCTCT CCAGGGAGGT CAGTGGAACC GAGTGCTGAA
    GAGAAAAGTT TAATCAGTGA TAAAACAGCC GAATGGATTG CAGAGGAGGA GGATGACGTG
    TTTGTGGCTT CCCGCACAAC TGAAGATTTA TTTACTGTGA TACACAGGTC CAAAAGAAAG
    CTACTGGGCT GGAAAGAGAC TGGGGAGGGC TTCACGGGAA GCAAACCCAG CTCCCATTCA
    CCAGTGAAGA ATACAGCTGA TTCTCCCACC GGTGAGGCTG CTGCTGCCCC AGGGCCAAGT
    AGCAGTGCCT GCCTAGATGC TGGTAGAAAT GATGATTTCA AGGCCTTGCT CCAGAAGAAG
    GGAAGTAAGG CAACTCCCAG GACCCGCCCC TCAGCGGCCG AGCTGCTGAA GACCACTAAC
    CCTCTGGCTC GAAGGATTAT TGCACAGTTC TCAAAAGACT ACGAACCCAC TGACAACCCC
    AGTACCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu75774
    Clone ID Related Accession (Same CDS sequence) XM_011247501.3 , XM_011247501.2
    Accession Version XM_011247501.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3252bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-21
    Organism Mus musculus(house mouse)
    Product NHS-like protein 2 isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000086.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_011247501.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGCCGTTCT ACCGGCGCAC TGTGGTGCCC CAGCGTCTGT GCCCGCGCAA CCCTCCGCAG 
    CCGCTGGCTG AGCTCCGCGA CGTGAGCCAC CTGGCCGCGC TCAGTCTGCT CCGCCAGCTC
    GCAGACCTCT GCGGCCACTC GCTGGCTCTG CTGGAGGACC TCGAGGGGCA CCTGCTGGCT
    CTGGGGCGCC GCACCGACAG CCTGTACCGG CGCACCGTCC GGCTCCGCCG CCGCCTTCCC
    TGTCGCCTGC TTGGCCCGGA GGATGATGAA GAATTGCTAG GTGCCTGCCA CTGGGTGAAC
    GGGACTCGCA TCCTGAGGGC AAGGGAGCTT GCTGATGTAG CCAGCACAGG GCAGCAGTTT
    GATAGACATG CAAGTTTTCG ACACTCGTTG TTTAACACAG AGACAGCCGT GAACCCCAAG
    TCCACCCTGA GGCGGAGGCG GACCATTATT GGATTCTCTA ACTTTTCTCA GCGAGACCAA
    GGTCACAGCA GCAGCCCCAC AGGCAGCCTG GCCCGCTCTG CCACCTCAGA CATCAGGCCT
    GGCCACTCAG CTCCACAAGT TGTTCAGGGA AGAGTTGCTG TTGGGCAGGA AGCTCGGTTC
    CCAAGTCTCA CCTCACCAGG ACTGAGACAC TCTTCTAGTG AGCCCGGAGA CGCTCACCAG
    GCACGCAGTG CCTCAGACCA TCCCGGCATG GAGAGCATGG CAGTGGTGTA CAGTGTCCCC
    AGTTCTTGCA ATGGACCAAC AGAGTCAACA TTCTCCACTT CCTGGAAGGG AGATGCTTTC
    ACATACATGA CCCCAAGTGC CAGCAGCCAG GGCAATCAAG TCAGTGAAAA TGGAAAAAAC
    CCTTCCTCGG GCAATTCTTG GGTCCCTCTG AACACACTCC CACCTCTGGT TCCTAAGGAG
    GCGGCCACGC TCTTTGTCAC CCGTGATAAC CCAGCAGGAT GCACTGGGCT ACCCAGCTAC
    TCTGAGCACC CCACTCTACG AAGACAGATA CCAGAAAGAC CTCCCAAGAT TGGCCTTCTT
    GCCCGTGGTA CCTCAAGGCT GGAAACAGGC CCAGGTGGGA CCAACAGGTT CCGGGAGCGG
    TCACTGTCTG TACCCACAGA CTCGGGTGTT ACCTCAGTGG ACTATGACGA AGAACAGAAG
    ACCAGTGAGA CCCGCATCCT GCCTTATGCC AGTACAAGCT CCGAGGGCAG TAACAGTACT
    GACAACATTG CAGCCCTCAG CACTGAGCAG GAGGCACGGC ACAGAAGGCA AAGATCCAAA
    AGTATTTCAC TCAAGAAGGC CAAAAAGAAG CCCTCTCCAC CCATGCGAAG TGTCTCACTC
    GTCAAAGATG AGCCAGCCCT CCCACCAGAA GGCGAATTGG TACTGCCCAA GGACCAGAGG
    CCTAGGAGCC TTTGCCTCTC CTTGGAACAC CAAGGGCACC ACCCACCTCA CCCAGATGCT
    CAAGGTCACC CAGCTGTGCC AATGCTCAAA GATCCAGGAA GTACGCAGTT CTCTCACCAC
    TGGTATCTTA CTGACTGGAA GTCTGGTGAC ACCTACCAGT CCTTGTCCAG CTCCAGCACT
    GCCACCGGCA CCACGGTCAT TGAGTGCACT CAAGTTCAGG GAAGCTCAGA GTCTCTGGCC
    TCCCCTTCCA CCTCCAGAGC GACAACACCC TCCCAGCTCT CCATTGAGGT GGAAGCCAGG
    GAAGTAGCCT CTCCTGGGAG GCCCACTGGA CTGATGTCCC CTTCCAGTGG TTACTCCAGC
    CAGTCAGAGA CACCCACACC CACTGTCTCC ATGTCCTTGA CCCTGGGCCA CTTACCTCCT
    CCAAGTGCTA GTGTCAGAGT ACGTCCAGTG GTACCAGAAA GGAAGTCATC ACTGCCCCCA
    ACATCACCAA TGGAGAAAAT CTGCAAGTCA CGGTTATCAT TTGACCTACC ACTGACCTCT
    TCAACCACCC TGGATCTGTC GGGGATGAGT ATCTCCATCC GCAGCAAAAC CAAGGTGAGC
    CGCCATCACT CTGATACCAA TTTTGGGGTC AAACTGGCAC AGAAAACTAG TCCAAACCAA
    CCGATCATGC CCATGGTTAC TCAGTCTGAC CTCCGTTCTG TTCGCCTGAG GTCAGTCAGC
    AAGTCTGAGC CAGAAGACGA CATTGAGAGT CCAGATTACA TAGAGGAACC TGGAGCAGAG
    GAAGTCTTCA CCATGCCAGA GAGAAAAGTG AAACCTCCCA TAGCCGAGAA ACCTCCATTG
    GCCCGAAGGC CTCCAAGTTT GGTCCACAGG CCGCCCTCTC TCCCTGGGGA GTATCCACTA
    ACTTCTCCTA CTATGGCTAT GGCATCCAGG AGCTCCATTC CACACATGAA GCAGCTTCCC
    CAAGACAGCT ACACAGTGTT GCGGAAACCA AAGTCACCCA GCTTCCCCGG GGAGTCAACA
    GCATCCTCCA GCCTTGTCCT CTCACCTCTT GCCAGTTCCT CTGGTGCTTT CTTCTCAGGA
    ACACAGCAAC CTCCCCAGGC CAGTGTAGAA GACGGGGGCC CCAAGGTGAG AGCCCTGCCT
    GAAAGAATCG GCCTCCAGAG CCAGGAAGAA GCTGAGAAAA AGATGACCAA GATTCCACCT
    CCGGTACCAA AAAAGCCCAG TGTGCTCTAC CTGCCTCTCA CCTCCCCTGT AGCTCAAATG
    GATGCCTGCA TGGCAGAACC AAGGCTACCT TTCAGCCCCA TCATCACCCT GGAGGAAGAT
    GGCAAGTGTC CCTCCACTGG TGATGACCAG AAGTCACCTG GTAAAGGGGT GACTTCGCCT
    CTTCACACTG ACACAGAAAA AGAGGCCATC TCTCCAGGGA GGTCAGTGGA ACCGAGTGCT
    GAAGAGAAAA GTTTAATCAG TGATAAAACA GCCGAATGGA TTGCAGAGGA GGAGGATGAC
    GTGTTTGTGG CTTCCCGCAC AACTGAAGAT TTATTTACTG TGATACACAG GTCCAAAAGA
    AAGCTACTGG GCTGGAAAGA GACTGGGGAG GGCTTCACGG GAAGCAAACC CAGCTCCCAT
    TCACCAGTGA AGAATACAGC TGATTCTCCC ACCGGTGAGG CTGCTGCTGC CCCAGGGCCA
    AGTAGCAGTG CCTGCCTAGA TGCTGGTAGA AATGATGATT TCAAGGCCTT GCTCCAGAAG
    AAGGGAAGTA AGGCAACTCC CAGGACCCGC CCCTCAGCGG CCGAGCTGCT GAAGACCACT
    AACCCTCTGG CTCGAAGGAT TATTGCACAG TTCTCAAAAG ACTACGAACC CACTGACAAC
    CCCAGTACCT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011245803.1
    CDS890..4141
    Translation

    Target ORF information:

    RefSeq Version XM_011247501.2
    Organism Mus musculus(house mouse)
    Definition Mus musculus NHS-like 2 (Nhsl2), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011247501.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGCCGTTCT ACCGGCGCAC TGTGGTGCCC CAGCGTCTGT GCCCGCGCAA CCCTCCGCAG 
    CCGCTGGCTG AGCTCCGCGA CGTGAGCCAC CTGGCCGCGC TCAGTCTGCT CCGCCAGCTC
    GCAGACCTCT GCGGCCACTC GCTGGCTCTG CTGGAGGACC TCGAGGGGCA CCTGCTGGCT
    CTGGGGCGCC GCACCGACAG CCTGTACCGG CGCACCGTCC GGCTCCGCCG CCGCCTTCCC
    TGTCGCCTGC TTGGCCCGGA GGATGATGAA GAATTGCTAG GTGCCTGCCA CTGGGTGAAC
    GGGACTCGCA TCCTGAGGGC AAGGGAGCTT GCTGATGTAG CCAGCACAGG GCAGCAGTTT
    GATAGACATG CAAGTTTTCG ACACTCGTTG TTTAACACAG AGACAGCCGT GAACCCCAAG
    TCCACCCTGA GGCGGAGGCG GACCATTATT GGATTCTCTA ACTTTTCTCA GCGAGACCAA
    GGTCACAGCA GCAGCCCCAC AGGCAGCCTG GCCCGCTCTG CCACCTCAGA CATCAGGCCT
    GGCCACTCAG CTCCACAAGT TGTTCAGGGA AGAGTTGCTG TTGGGCAGGA AGCTCGGTTC
    CCAAGTCTCA CCTCACCAGG ACTGAGACAC TCTTCTAGTG AGCCCGGAGA CGCTCACCAG
    GCACGCAGTG CCTCAGACCA TCCCGGCATG GAGAGCATGG CAGTGGTGTA CAGTGTCCCC
    AGTTCTTGCA ATGGACCAAC AGAGTCAACA TTCTCCACTT CCTGGAAGGG AGATGCTTTC
    ACATACATGA CCCCAAGTGC CAGCAGCCAG GGCAATCAAG TCAGTGAAAA TGGAAAAAAC
    CCTTCCTCGG GCAATTCTTG GGTCCCTCTG AACACACTCC CACCTCTGGT TCCTAAGGAG
    GCGGCCACGC TCTTTGTCAC CCGTGATAAC CCAGCAGGAT GCACTGGGCT ACCCAGCTAC
    TCTGAGCACC CCACTCTACG AAGACAGATA CCAGAAAGAC CTCCCAAGAT TGGCCTTCTT
    GCCCGTGGTA CCTCAAGGCT GGAAACAGGC CCAGGTGGGA CCAACAGGTT CCGGGAGCGG
    TCACTGTCTG TACCCACAGA CTCGGGTGTT ACCTCAGTGG ACTATGACGA AGAACAGAAG
    ACCAGTGAGA CCCGCATCCT GCCTTATGCC AGTACAAGCT CCGAGGGCAG TAACAGTACT
    GACAACATTG CAGCCCTCAG CACTGAGCAG GAGGCACGGC ACAGAAGGCA AAGATCCAAA
    AGTATTTCAC TCAAGAAGGC CAAAAAGAAG CCCTCTCCAC CCATGCGAAG TGTCTCACTC
    GTCAAAGATG AGCCAGCCCT CCCACCAGAA GGCGAATTGG TACTGCCCAA GGACCAGAGG
    CCTAGGAGCC TTTGCCTCTC CTTGGAACAC CAAGGGCACC ACCCACCTCA CCCAGATGCT
    CAAGGTCACC CAGCTGTGCC AATGCTCAAA GATCCAGGAA GTACGCAGTT CTCTCACCAC
    TGGTATCTTA CTGACTGGAA GTCTGGTGAC ACCTACCAGT CCTTGTCCAG CTCCAGCACT
    GCCACCGGCA CCACGGTCAT TGAGTGCACT CAAGTTCAGG GAAGCTCAGA GTCTCTGGCC
    TCCCCTTCCA CCTCCAGAGC GACAACACCC TCCCAGCTCT CCATTGAGGT GGAAGCCAGG
    GAAGTAGCCT CTCCTGGGAG GCCCACTGGA CTGATGTCCC CTTCCAGTGG TTACTCCAGC
    CAGTCAGAGA CACCCACACC CACTGTCTCC ATGTCCTTGA CCCTGGGCCA CTTACCTCCT
    CCAAGTGCTA GTGTCAGAGT ACGTCCAGTG GTACCAGAAA GGAAGTCATC ACTGCCCCCA
    ACATCACCAA TGGAGAAAAT CTGCAAGTCA CGGTTATCAT TTGACCTACC ACTGACCTCT
    TCAACCACCC TGGATCTGTC GGGGATGAGT ATCTCCATCC GCAGCAAAAC CAAGGTGAGC
    CGCCATCACT CTGATACCAA TTTTGGGGTC AAACTGGCAC AGAAAACTAG TCCAAACCAA
    CCGATCATGC CCATGGTTAC TCAGTCTGAC CTCCGTTCTG TTCGCCTGAG GTCAGTCAGC
    AAGTCTGAGC CAGAAGACGA CATTGAGAGT CCAGATTACA TAGAGGAACC TGGAGCAGAG
    GAAGTCTTCA CCATGCCAGA GAGAAAAGTG AAACCTCCCA TAGCCGAGAA ACCTCCATTG
    GCCCGAAGGC CTCCAAGTTT GGTCCACAGG CCGCCCTCTC TCCCTGGGGA GTATCCACTA
    ACTTCTCCTA CTATGGCTAT GGCATCCAGG AGCTCCATTC CACACATGAA GCAGCTTCCC
    CAAGACAGCT ACACAGTGTT GCGGAAACCA AAGTCACCCA GCTTCCCCGG GGAGTCAACA
    GCATCCTCCA GCCTTGTCCT CTCACCTCTT GCCAGTTCCT CTGGTGCTTT CTTCTCAGGA
    ACACAGCAAC CTCCCCAGGC CAGTGTAGAA GACGGGGGCC CCAAGGTGAG AGCCCTGCCT
    GAAAGAATCG GCCTCCAGAG CCAGGAAGAA GCTGAGAAAA AGATGACCAA GATTCCACCT
    CCGGTACCAA AAAAGCCCAG TGTGCTCTAC CTGCCTCTCA CCTCCCCTGT AGCTCAAATG
    GATGCCTGCA TGGCAGAACC AAGGCTACCT TTCAGCCCCA TCATCACCCT GGAGGAAGAT
    GGCAAGTGTC CCTCCACTGG TGATGACCAG AAGTCACCTG GTAAAGGGGT GACTTCGCCT
    CTTCACACTG ACACAGAAAA AGAGGCCATC TCTCCAGGGA GGTCAGTGGA ACCGAGTGCT
    GAAGAGAAAA GTTTAATCAG TGATAAAACA GCCGAATGGA TTGCAGAGGA GGAGGATGAC
    GTGTTTGTGG CTTCCCGCAC AACTGAAGAT TTATTTACTG TGATACACAG GTCCAAAAGA
    AAGCTACTGG GCTGGAAAGA GACTGGGGAG GGCTTCACGG GAAGCAAACC CAGCTCCCAT
    TCACCAGTGA AGAATACAGC TGATTCTCCC ACCGGTGAGG CTGCTGCTGC CCCAGGGCCA
    AGTAGCAGTG CCTGCCTAGA TGCTGGTAGA AATGATGATT TCAAGGCCTT GCTCCAGAAG
    AAGGGAAGTA AGGCAACTCC CAGGACCCGC CCCTCAGCGG CCGAGCTGCT GAAGACCACT
    AACCCTCTGG CTCGAAGGAT TATTGCACAG TTCTCAAAAG ACTACGAACC CACTGACAAC
    CCCAGTACCT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu75775
    Clone ID Related Accession (Same CDS sequence) XM_011247502.2 , XM_011247502.3
    Accession Version XM_011247502.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3120bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-21
    Organism Mus musculus(house mouse)
    Product NHS-like protein 2 isoform X8
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000086.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_011247502.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    ATGCCGTTCT ACCGGCGCAC TGTGGTGCCC CAGCGTCTGT GCCCGCGCAA CCCTCCGCAG 
    CCGCTGGCTG AGCTCCGCGA CGTGAGCCAC CTGGCCGCGC TCAGTCTGCT CCGCCAGCTC
    GCAGACCTCT GCGGCCACTC GCTGGCTCTG CTGGAGGACC TCGAGGGGCA CCTGCTGGCT
    CTGGGGCGCC GCACCGACAG CCTGTACCGG CGCACCGTCC GGCTCCGCCG CCGCCTTCCC
    TGTCGCCTGC TTGGCCCGGA GGATGATGAA GAATTGCTAG GTGCCTGCCA CTGGGTGAAC
    GGGACTCGCA TCCTGAGGGC AAGGGAGCTT GCTGATGTAG CCAGCACAGG TCACAGCAGC
    AGCCCCACAG GCAGCCTGGC CCGCTCTGCC ACCTCAGACA TCAGGCCTGG CCACTCAGCT
    CCACAAGTTG TTCAGGGAAG AGTTGCTGTT GGGCAGGAAG CTCGGTTCCC AAGTCTCACC
    TCACCAGGAC TGAGACACTC TTCTAGTGAG CCCGGAGACG CTCACCAGGC ACGCAGTGCC
    TCAGACCATC CCGGCATGGA GAGCATGGCA GTGGTGTACA GTGTCCCCAG TTCTTGCAAT
    GGACCAACAG AGTCAACATT CTCCACTTCC TGGAAGGGAG ATGCTTTCAC ATACATGACC
    CCAAGTGCCA GCAGCCAGGG CAATCAAGTC AGTGAAAATG GAAAAAACCC TTCCTCGGGC
    AATTCTTGGG TCCCTCTGAA CACACTCCCA CCTCTGGTTC CTAAGGAGGC GGCCACGCTC
    TTTGTCACCC GTGATAACCC AGCAGGATGC ACTGGGCTAC CCAGCTACTC TGAGCACCCC
    ACTCTACGAA GACAGATACC AGAAAGACCT CCCAAGATTG GCCTTCTTGC CCGTGGTACC
    TCAAGGCTGG AAACAGGCCC AGGTGGGACC AACAGGTTCC GGGAGCGGTC ACTGTCTGTA
    CCCACAGACT CGGGTGTTAC CTCAGTGGAC TATGACGAAG AACAGAAGAC CAGTGAGACC
    CGCATCCTGC CTTATGCCAG TACAAGCTCC GAGGGCAGTA ACAGTACTGA CAACATTGCA
    GCCCTCAGCA CTGAGCAGGA GGCACGGCAC AGAAGGCAAA GATCCAAAAG TATTTCACTC
    AAGAAGGCCA AAAAGAAGCC CTCTCCACCC ATGCGAAGTG TCTCACTCGT CAAAGATGAG
    CCAGCCCTCC CACCAGAAGG CGAATTGGTA CTGCCCAAGG ACCAGAGGCC TAGGAGCCTT
    TGCCTCTCCT TGGAACACCA AGGGCACCAC CCACCTCACC CAGATGCTCA AGGTCACCCA
    GCTGTGCCAA TGCTCAAAGA TCCAGGAAGT ACGCAGTTCT CTCACCACTG GTATCTTACT
    GACTGGAAGT CTGGTGACAC CTACCAGTCC TTGTCCAGCT CCAGCACTGC CACCGGCACC
    ACGGTCATTG AGTGCACTCA AGTTCAGGGA AGCTCAGAGT CTCTGGCCTC CCCTTCCACC
    TCCAGAGCGA CAACACCCTC CCAGCTCTCC ATTGAGGTGG AAGCCAGGGA AGTAGCCTCT
    CCTGGGAGGC CCACTGGACT GATGTCCCCT TCCAGTGGTT ACTCCAGCCA GTCAGAGACA
    CCCACACCCA CTGTCTCCAT GTCCTTGACC CTGGGCCACT TACCTCCTCC AAGTGCTAGT
    GTCAGAGTAC GTCCAGTGGT ACCAGAAAGG AAGTCATCAC TGCCCCCAAC ATCACCAATG
    GAGAAAATCT GCAAGTCACG GTTATCATTT GACCTACCAC TGACCTCTTC AACCACCCTG
    GATCTGTCGG GGATGAGTAT CTCCATCCGC AGCAAAACCA AGGTGAGCCG CCATCACTCT
    GATACCAATT TTGGGGTCAA ACTGGCACAG AAAACTAGTC CAAACCAACC GATCATGCCC
    ATGGTTACTC AGTCTGACCT CCGTTCTGTT CGCCTGAGGT CAGTCAGCAA GTCTGAGCCA
    GAAGACGACA TTGAGAGTCC AGATTACATA GAGGAACCTG GAGCAGAGGA AGTCTTCACC
    ATGCCAGAGA GAAAAGTGAA ACCTCCCATA GCCGAGAAAC CTCCATTGGC CCGAAGGCCT
    CCAAGTTTGG TCCACAGGCC GCCCTCTCTC CCTGGGGAGT ATCCACTAAC TTCTCCTACT
    ATGGCTATGG CATCCAGGAG CTCCATTCCA CACATGAAGC AGCTTCCCCA AGACAGCTAC
    ACAGTGTTGC GGAAACCAAA GTCACCCAGC TTCCCCGGGG AGTCAACAGC ATCCTCCAGC
    CTTGTCCTCT CACCTCTTGC CAGTTCCTCT GGTGCTTTCT TCTCAGGAAC ACAGCAACCT
    CCCCAGGCCA GTGTAGAAGA CGGGGGCCCC AAGGTGAGAG CCCTGCCTGA AAGAATCGGC
    CTCCAGAGCC AGGAAGAAGC TGAGAAAAAG ATGACCAAGA TTCCACCTCC GGTACCAAAA
    AAGCCCAGTG TGCTCTACCT GCCTCTCACC TCCCCTGTAG CTCAAATGGA TGCCTGCATG
    GCAGAACCAA GGCTACCTTT CAGCCCCATC ATCACCCTGG AGGAAGATGG CAAGTGTCCC
    TCCACTGGTG ATGACCAGAA GTCACCTGGT AAAGGGGTGA CTTCGCCTCT TCACACTGAC
    ACAGAAAAAG AGGCCATCTC TCCAGGGAGG TCAGTGGAAC CGAGTGCTGA AGAGAAAAGT
    TTAATCAGTG ATAAAACAGC CGAATGGATT GCAGAGGAGG AGGATGACGT GTTTGTGGCT
    TCCCGCACAA CTGAAGATTT ATTTACTGTG ATACACAGGT CCAAAAGAAA GCTACTGGGC
    TGGAAAGAGA CTGGGGAGGG CTTCACGGGA AGCAAACCCA GCTCCCATTC ACCAGTGAAG
    AATACAGCTG ATTCTCCCAC CGGTGAGGCT GCTGCTGCCC CAGGGCCAAG TAGCAGTGCC
    TGCCTAGATG CTGGTAGAAA TGATGATTTC AAGGCCTTGC TCCAGAAGAA GGGAAGTAAG
    GCAACTCCCA GGACCCGCCC CTCAGCGGCC GAGCTGCTGA AGACCACTAA CCCTCTGGCT
    CGAAGGATTA TTGCACAGTT CTCAAAAGAC TACGAACCCA CTGACAACCC CAGTACCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011245804.1
    CDS888..4007
    Translation

    Target ORF information:

    RefSeq Version XM_011247502.2
    Organism Mus musculus(house mouse)
    Definition Mus musculus NHS-like 2 (Nhsl2), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011247502.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    ATGCCGTTCT ACCGGCGCAC TGTGGTGCCC CAGCGTCTGT GCCCGCGCAA CCCTCCGCAG 
    CCGCTGGCTG AGCTCCGCGA CGTGAGCCAC CTGGCCGCGC TCAGTCTGCT CCGCCAGCTC
    GCAGACCTCT GCGGCCACTC GCTGGCTCTG CTGGAGGACC TCGAGGGGCA CCTGCTGGCT
    CTGGGGCGCC GCACCGACAG CCTGTACCGG CGCACCGTCC GGCTCCGCCG CCGCCTTCCC
    TGTCGCCTGC TTGGCCCGGA GGATGATGAA GAATTGCTAG GTGCCTGCCA CTGGGTGAAC
    GGGACTCGCA TCCTGAGGGC AAGGGAGCTT GCTGATGTAG CCAGCACAGG TCACAGCAGC
    AGCCCCACAG GCAGCCTGGC CCGCTCTGCC ACCTCAGACA TCAGGCCTGG CCACTCAGCT
    CCACAAGTTG TTCAGGGAAG AGTTGCTGTT GGGCAGGAAG CTCGGTTCCC AAGTCTCACC
    TCACCAGGAC TGAGACACTC TTCTAGTGAG CCCGGAGACG CTCACCAGGC ACGCAGTGCC
    TCAGACCATC CCGGCATGGA GAGCATGGCA GTGGTGTACA GTGTCCCCAG TTCTTGCAAT
    GGACCAACAG AGTCAACATT CTCCACTTCC TGGAAGGGAG ATGCTTTCAC ATACATGACC
    CCAAGTGCCA GCAGCCAGGG CAATCAAGTC AGTGAAAATG GAAAAAACCC TTCCTCGGGC
    AATTCTTGGG TCCCTCTGAA CACACTCCCA CCTCTGGTTC CTAAGGAGGC GGCCACGCTC
    TTTGTCACCC GTGATAACCC AGCAGGATGC ACTGGGCTAC CCAGCTACTC TGAGCACCCC
    ACTCTACGAA GACAGATACC AGAAAGACCT CCCAAGATTG GCCTTCTTGC CCGTGGTACC
    TCAAGGCTGG AAACAGGCCC AGGTGGGACC AACAGGTTCC GGGAGCGGTC ACTGTCTGTA
    CCCACAGACT CGGGTGTTAC CTCAGTGGAC TATGACGAAG AACAGAAGAC CAGTGAGACC
    CGCATCCTGC CTTATGCCAG TACAAGCTCC GAGGGCAGTA ACAGTACTGA CAACATTGCA
    GCCCTCAGCA CTGAGCAGGA GGCACGGCAC AGAAGGCAAA GATCCAAAAG TATTTCACTC
    AAGAAGGCCA AAAAGAAGCC CTCTCCACCC ATGCGAAGTG TCTCACTCGT CAAAGATGAG
    CCAGCCCTCC CACCAGAAGG CGAATTGGTA CTGCCCAAGG ACCAGAGGCC TAGGAGCCTT
    TGCCTCTCCT TGGAACACCA AGGGCACCAC CCACCTCACC CAGATGCTCA AGGTCACCCA
    GCTGTGCCAA TGCTCAAAGA TCCAGGAAGT ACGCAGTTCT CTCACCACTG GTATCTTACT
    GACTGGAAGT CTGGTGACAC CTACCAGTCC TTGTCCAGCT CCAGCACTGC CACCGGCACC
    ACGGTCATTG AGTGCACTCA AGTTCAGGGA AGCTCAGAGT CTCTGGCCTC CCCTTCCACC
    TCCAGAGCGA CAACACCCTC CCAGCTCTCC ATTGAGGTGG AAGCCAGGGA AGTAGCCTCT
    CCTGGGAGGC CCACTGGACT GATGTCCCCT TCCAGTGGTT ACTCCAGCCA GTCAGAGACA
    CCCACACCCA CTGTCTCCAT GTCCTTGACC CTGGGCCACT TACCTCCTCC AAGTGCTAGT
    GTCAGAGTAC GTCCAGTGGT ACCAGAAAGG AAGTCATCAC TGCCCCCAAC ATCACCAATG
    GAGAAAATCT GCAAGTCACG GTTATCATTT GACCTACCAC TGACCTCTTC AACCACCCTG
    GATCTGTCGG GGATGAGTAT CTCCATCCGC AGCAAAACCA AGGTGAGCCG CCATCACTCT
    GATACCAATT TTGGGGTCAA ACTGGCACAG AAAACTAGTC CAAACCAACC GATCATGCCC
    ATGGTTACTC AGTCTGACCT CCGTTCTGTT CGCCTGAGGT CAGTCAGCAA GTCTGAGCCA
    GAAGACGACA TTGAGAGTCC AGATTACATA GAGGAACCTG GAGCAGAGGA AGTCTTCACC
    ATGCCAGAGA GAAAAGTGAA ACCTCCCATA GCCGAGAAAC CTCCATTGGC CCGAAGGCCT
    CCAAGTTTGG TCCACAGGCC GCCCTCTCTC CCTGGGGAGT ATCCACTAAC TTCTCCTACT
    ATGGCTATGG CATCCAGGAG CTCCATTCCA CACATGAAGC AGCTTCCCCA AGACAGCTAC
    ACAGTGTTGC GGAAACCAAA GTCACCCAGC TTCCCCGGGG AGTCAACAGC ATCCTCCAGC
    CTTGTCCTCT CACCTCTTGC CAGTTCCTCT GGTGCTTTCT TCTCAGGAAC ACAGCAACCT
    CCCCAGGCCA GTGTAGAAGA CGGGGGCCCC AAGGTGAGAG CCCTGCCTGA AAGAATCGGC
    CTCCAGAGCC AGGAAGAAGC TGAGAAAAAG ATGACCAAGA TTCCACCTCC GGTACCAAAA
    AAGCCCAGTG TGCTCTACCT GCCTCTCACC TCCCCTGTAG CTCAAATGGA TGCCTGCATG
    GCAGAACCAA GGCTACCTTT CAGCCCCATC ATCACCCTGG AGGAAGATGG CAAGTGTCCC
    TCCACTGGTG ATGACCAGAA GTCACCTGGT AAAGGGGTGA CTTCGCCTCT TCACACTGAC
    ACAGAAAAAG AGGCCATCTC TCCAGGGAGG TCAGTGGAAC CGAGTGCTGA AGAGAAAAGT
    TTAATCAGTG ATAAAACAGC CGAATGGATT GCAGAGGAGG AGGATGACGT GTTTGTGGCT
    TCCCGCACAA CTGAAGATTT ATTTACTGTG ATACACAGGT CCAAAAGAAA GCTACTGGGC
    TGGAAAGAGA CTGGGGAGGG CTTCACGGGA AGCAAACCCA GCTCCCATTC ACCAGTGAAG
    AATACAGCTG ATTCTCCCAC CGGTGAGGCT GCTGCTGCCC CAGGGCCAAG TAGCAGTGCC
    TGCCTAGATG CTGGTAGAAA TGATGATTTC AAGGCCTTGC TCCAGAAGAA GGGAAGTAAG
    GCAACTCCCA GGACCCGCCC CTCAGCGGCC GAGCTGCTGA AGACCACTAA CCCTCTGGCT
    CGAAGGATTA TTGCACAGTT CTCAAAAGAC TACGAACCCA CTGACAACCC CAGTACCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.