Kcnj13 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Kcnj13
Entrez Gene ID	100040591
Full Name	potassium inwardly-rectifying channel, subfamily J, member 13
General protein information	Preferred Names potassium inwardly-rectifying channel, subfamily J, member 13 Names inward rectifier potassium channel 13 inward rectifier K(+) channel Kir7.1
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 1 Map Location: 1 C5\|1

mRNA and Protein(s)

mRNA	Protein	Name
NM_001110227.2	NP_001103697.1	inward rectifier potassium channel 13

Pathways from BioSystems

Homologies

Gallus gallus (chicken)	KCNJ13	XP_004936982.1
Xenopus tropicalis (tropical clawed frog)	kcnj13	NP_001096437.1
Rattus norvegicus (Norway rat)	Kcnj13	NP_446060.1
Homo sapiens (human)	KCNJ13	NP_002233.2
	KCNJ13	XP_001114542.1
Pan troglodytes (chimpanzee)	KCNJ13	XP_003309569.1
Bos taurus (cattle)	KCNJ13	NP_001180183.1
Canis lupus familiaris (dog)	KCNJ13	XP_005635917.1
Mus musculus (house mouse)	Kcnj13	NP_001103697.1
Danio rerio (zebrafish)	kcnj13	NP_001039014.1

Gene Ontology
Bibliography

Related articles in PubMed

G-protein-independent coupling of MC4R to Kir7.1 in hypothalamic neurons.
Ghamari-Langroudi M, Digby GJ, Sebag JA, Millhauser GL, Palomino R, Matthews R, Gillyard T, Panaro BL, Tough IR, Cox HM, Denton JS, Cone RD
Nature520(7545)94-8(2015 Apr)

CRISPR-engineered mosaicism rapidly reveals that loss of Kcnj13 function in mice mimics human disease phenotypes.
Zhong H, Chen Y, Li Y, Chen R, Mardon G
Scientific reports58366(2015 Feb)

Cleft Palate, Moderate Lung Developmental Retardation and Early Postnatal Lethality in Mice Deficient in the Kir7.1 Inwardly Rectifying K+ Channel.
Villanueva S, Burgos J, L?pez-Cayuqueo KI, Lai KM, Valenzuela DM, Cid LP, Sep?lveda FV
PloS one10(9)e0139284(2015)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Coupling of MC4R to Kir7.1 may explain unusual aspects of the control of energy homeostasis by melanocortin signalling, including the gene dosage effect of MC4R and the sustained effects of AgRP on food intake.
Title: G-protein-independent coupling of MC4R to Kir7.1 in hypothalamic neurons.

These results suggest that KCNJ13 expression is required for RPE cells to maintain photoreceptor survival.
Title: CRISPR-engineered mosaicism rapidly reveals that loss of Kcnj13 function in mice mimics human disease phenotypes.

Kir7.1 regulates the transition from quiescence to contractions in the pregnant uterus.
Title: The inwardly rectifying K+ channel KIR7.1 controls uterine excitability throughout pregnancy.

The following Kcnj13 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Kcnj13 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu04537	NM_001110227.2 Latest version!	Mus musculus potassium inwardly-rectifying channel, subfamily J, member 13 (Kcnj13), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Kcnj13 ( NM_001110227.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001103697.1 Mus musculus potassium inwardly-rectifying channel, subfamily J, member 13 (Kcnj13), mRNA.

Price & Availability↑

CloneID	OMu04537
Clone ID Related Accession (Same CDS sequence)	NM_001110227.2
Accession Version	NM_001110227.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1083bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-28
Organism	Mus musculus(house mouse)
Product	inward rectifier potassium channel 13
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from CJ131832.1, CK623705.1 and AC157811.3. On Sep 1, 2015 this sequence version replaced NM_001110227.1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: CJ132004.1, CJ131649.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164131, SAMN01164134 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGACAGCA GTAATTGTAA AGTGAATGCT CCTCTCCTGA GTCAAAGACA CCGGAGGATG 
GTCACAAAAG ACGGCCACAG CACACTTCAG ATGGATGGTG CTCAAAGGGG CCTTGTGTAT 
CTCCGAGATG CATGGGGAAT CCTAATGGAC ATGCGCTGGC GCTGGATGAT GCTGGTCTTT 
TCTGCTTCTT TTGTTGTCCA CTGGCTTGTC TTTGCAGTCC TCTGGTATGC TGTAGCTGAG 
ATGAATGGTG ATCTGGAAAT AGACCACGAC GTCCCACCTG AAAACCACAC TATCTGTGTG 
AAGCACATCA CCAGCTTCAC AGCTGCATTC TCCTTCTCTC TGGAGACACA ACTTACAATT 
GGTTATGGTA CCATGTTTCC CAGTGGTGAC TGTCCAAGTG CAATCGCCTT ACTTGCCATA 
CAAATGCTCC TAGGCCTCAT GCTAGAGGCT TTTATCACAG GTGCCTTTGT GGCGAAGATT 
GCACGGCCAA AAAATAGAGC TTTCTCAATT CGATTCACTG ACTTAGCAGT TGTAGCTCAC 
AAAGATGGCA AACCTAATCT TATCTTCCAA GTGGCCAACA CCCGGCCCAG CCCTCTGACC 
AACGTTCGTG TCTCTGCTGT ACTCTATCAG GAAAGAGAAA ATGGTGAACT TTACCAGACC 
AGTGTGGACT TCCACCTTGA TGGCATCAGT TCTGAGGAAT GTCCATTCTT CATCTTCCCG 
CTAACCTATT ACCATACTAT CTCACCATCA AGTCCTCTAG CTACCCTGCT CCAACATGAG 
ACTCCTCCAC ACTTTGAATT AGTTGTGTTC CTCTCAGCAA TGCAAGAAGG CACTGGGGAA 
ATCTGCCAAA GGAGGACATC CTACCTGCCC TCTGAGATCA TGTTACATCA CCGATTTGCA 
GCTCTAATGA CTCGAGGTTC CAAAGGTGAG TATCAAGTCA AGATGGAGAA TTTTGACAAG 
ACTGTTCCTG AACATCCAAC ACCTGTGGTC TCTAAGAGTC CACACAGGAC TGACCTAGAT 
ATTCATATCA ATGGACAAAG CATTGACAAT TTCCAGATTG CTGAAACAGG GCTGACAGAA 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001103697.1
CDS	135..1217
Translation	MDSSNCKVNAPLLSQRHRRMVTKDGHSTLQMDGAQRGLVYLRDAWGILMDMRWRWMMLVFSASFVVHWLVFAVLWYAVAEMNGDLEIDHDVPPENHTICVKHITSFTAAFSFSLETQLTIGYGTMFPSGDCPSAIALLAIQMLLGLMLEAFITGAFVAKIARPKNRAFSIRFTDLAVVAHKDGKPNLIFQVANTRPSPLTNVRVSAVLYQERENGELYQTSVDFHLDGISSEECPFFIFPLTYYHTISPSSPLATLLQHETPPHFELVVFLSAMQEGTGEICQRRTSYLPSEIMLHHRFAALMTRGSKGEYQVKMENFDKTVPEHPTPVVSKSPHRTDLDIHINGQSIDNFQIAETGLTE

Target ORF information:

RefSeq Version	NM_001110227.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus potassium inwardly-rectifying channel, subfamily J, member 13 (Kcnj13), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001110227.2

ORF Insert Sequence:

ATGGACAGCA GTAATTGTAA AGTGAATGCT CCTCTCCTGA GTCAAAGACA CCGGAGGATG 
GTCACAAAAG ACGGCCACAG CACACTTCAG ATGGATGGTG CTCAAAGGGG CCTTGTGTAT 
CTCCGAGATG CATGGGGAAT CCTAATGGAC ATGCGCTGGC GCTGGATGAT GCTGGTCTTT 
TCTGCTTCTT TTGTTGTCCA CTGGCTTGTC TTTGCAGTCC TCTGGTATGC TGTAGCTGAG 
ATGAATGGTG ATCTGGAAAT AGACCACGAC GTCCCACCTG AAAACCACAC TATCTGTGTG 
AAGCACATCA CCAGCTTCAC AGCTGCATTC TCCTTCTCTC TGGAGACACA ACTTACAATT 
GGTTATGGTA CCATGTTTCC CAGTGGTGAC TGTCCAAGTG CAATCGCCTT ACTTGCCATA 
CAAATGCTCC TAGGCCTCAT GCTAGAGGCT TTTATCACAG GTGCCTTTGT GGCGAAGATT 
GCACGGCCAA AAAATAGAGC TTTCTCAATT CGATTCACTG ACTTAGCAGT TGTAGCTCAC 
AAAGATGGCA AACCTAATCT TATCTTCCAA GTGGCCAACA CCCGGCCCAG CCCTCTGACC 
AACGTTCGTG TCTCTGCTGT ACTCTATCAG GAAAGAGAAA ATGGTGAACT TTACCAGACC 
AGTGTGGACT TCCACCTTGA TGGCATCAGT TCTGAGGAAT GTCCATTCTT CATCTTCCCG 
CTAACCTATT ACCATACTAT CTCACCATCA AGTCCTCTAG CTACCCTGCT CCAACATGAG 
ACTCCTCCAC ACTTTGAATT AGTTGTGTTC CTCTCAGCAA TGCAAGAAGG CACTGGGGAA 
ATCTGCCAAA GGAGGACATC CTACCTGCCC TCTGAGATCA TGTTACATCA CCGATTTGCA 
GCTCTAATGA CTCGAGGTTC CAAAGGTGAG TATCAAGTCA AGATGGAGAA TTTTGACAAG 
ACTGTTCCTG AACATCCAAC ACCTGTGGTC TCTAAGAGTC CACACAGGAC TGACCTAGAT 
ATTCATATCA ATGGACAAAG CATTGACAAT TTCCAGATTG CTGAAACAGG GCTGACAGAA 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

G-protein-independent coupling of MC4R to Kir7.1 in hypothalamic neurons.
Nature520(7545)94-8(2015 Apr)
Ghamari-Langroudi M,Digby GJ,Sebag JA,Millhauser GL,Palomino R,Matthews R,Gillyard T,Panaro BL,Tough IR,Cox HM,Denton JS,Cone RD

CRISPR-engineered mosaicism rapidly reveals that loss of Kcnj13 function in mice mimics human disease phenotypes.
Scientific reports58366(2015 Feb)
Zhong H,Chen Y,Li Y,Chen R,Mardon G

Cleft Palate, Moderate Lung Developmental Retardation and Early Postnatal Lethality in Mice Deficient in the Kir7.1 Inwardly Rectifying K+ Channel.
PloS one10(9)e0139284(2015)
Villanueva S,Burgos J,L?pez-Cayuqueo KI,Lai KM,Valenzuela DM,Cid LP,Sep?lveda FV