Rasa1 cDNA ORF clone, Mus caroli(Ryukyu mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Rasa1
Entrez Gene ID	110308133
Full Name	RAS p21 protein activator 1
Gene Type	protein-coding
Organism	Mus caroli(Ryukyu mouse)

mRNA and Protein(s)

mRNA	Protein	Name
XM_021180504.1	XP_021036163.1	ras GTPase-activating protein 1
XM_021180504.2	XP_021036163.1	ras GTPase-activating protein 1

Pathways from BioSystems
Gene Ontology

The following Rasa1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Rasa1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMv29923	XM_021180504.2 Latest version!	Mus caroli RAS p21 protein activator 1 (Rasa1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$629.30 $899.00
View Old Accession Versions >>
OMv29923	XM_021180504.1	Mus caroli RAS p21 protein activator 1 (Rasa1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$629.30 $899.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Rasa1 ( XM_021180504.1 ) cDNA ORF clone, Mus caroli(Ryukyu mouse) -> XP_021036163.1 Mus caroli RAS p21 protein activator 1 (Rasa1), mRNA.

Price & Availability↑

CloneID	OMv29923
Clone ID Related Accession (Same CDS sequence)	XM_021180504.1 , XM_021180504.2
Accession Version	XM_021180504.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3117bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-05-20
Organism	Mus caroli(Ryukyu mouse)
Product	ras GTPase-activating protein 1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_034582.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus caroli Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGGCGG CCGAGGCGGG CAGTGAGGAA GGCGGCCCTG CGACGGCCGG GGCCGGCGGC 
GGCGCGGCCA CGGGCTCCAG CGCCTACTCG GCAGCGTGCC GGGTCAAGTT ACCCGCGGCC 
CTGCCGGTGG CAGCCGCCCC TTGTCCCGGG CTGGCGGAGG CCGACTTGGT TGCGGCCCTC 
GGTGGCGGAG CCGCGTCGGG GTCCGGGTTC CTGGGGACCG GACCGGTGGC CGGTGTCTTG 
GGGGGCGCCG CGCTGACCGG CAGTGCTGCT GCTGGCGTGG CTGGTGCTGC GGCCGCCGGA 
CCTGCTGGAG ACGTCGCTTT CACCAAGGGG ACTCTGTCTT TGCCTGCTGA GACGCTCGGG 
CCGGGCGGCG GATTCCCTCC TCTGCCGCCG CCTCCTCAGC TGCCCCCTTT GGGCTCCGGT 
CTGGGGACCG TGGATGAAGG TGACTCCCTG GATGGACCAG AATACGAAGA GGAAGAGGTG 
GCCATACCGC TGACCGCACC TCCCACTAAC CAGTGGTATC ATGGAAAACT TGATAGAACT 
ATAGCAGAAG AACGCCTCAG GCAGGCAGGG AAATCTGGAA GCTATCTTAT AAGAGAGAGT 
GATCGGAGGC CGGGGTCCTT TGTCCTTTCA TTCCTTAGTC AGACAAATGT TGTCAATCAC 
TTTAGGATTA TTGCTATGTG TGGTGATTAC TACATTGGTG GAAGGCGATT TTCTTCACTC 
TCAGACCTAA TAGGTTATTA CAGTCATGTT TCTTGTTTAC TTAAAGGAGA AAAATTACTC 
TATCCAGTTG CACCACCAGA GCCAGTAGAA GATAGAAGGC GTGTGCGAGC CATTCTGCCC 
TACACAAAAG TACCAGACAC TGATGAAATA AGTTTCTTAA AAGGAGATAT GTTCATTGTT 
CACAATGAGT TAGAAGATGG ATGGATGTGG GTTACCAATC TAAGAACAGA TGAGCAAGGC 
CTTATTGTTG AAGACCTAGT GGAAGAGGTG AGCCGAGAAG AAGATCCACA TGAAGGCAAA 
ATATGGTTCC ACGGGAAAAT TTCCAAACAA GAAGCTTATA ATTTATTAAT GACAGTTGGA 
CAAGTCTGCA GCTTTCTTGT GAGGCCCTCA GATAATACTC CTGGAGATTA TTCCCTGTAT 
TTTCGGACCA ATGAAAATAT TCAGCGATTT AAAATTTGTC CAACACCTAA CAACCAGTTT 
ATGATGGGAG GCCGCTATTA TAACAGTATT GGGGACATCA TAGACCATTA TCGGAAAGAA 
CAAATTGTTG AAGGCTATTA TCTTAAGGAA CCTGTACCAA TGCAGGATCA AGGGCAAGTA 
CTCAATGACA CAGTGGATGG GAAGGAAATC TATAATACAA TTCGTCGTAA AACAAAGGAC 
GCTTTTTATA AAAATATTGT TAAGAAAGGT TATCTTCTGA AGAAGGGTAA AGGAAAACGT 
TGGAAAAATT TATATTTTAT CTTAGAAGGC AGTGATGCTC AACTTATTTA TTTTGAAAGT 
GAAAAACGAG CTACAAAACC AAAAGGGCTA ATAGATCTTA GTGTGTGTTC TGTCTATGTT 
GTCCATGACA GTCTCTTCGG CAGGCCAAAC TGTTTTCAAA TAGTAGTTCA GCACTTTAGT 
GAAGAACATT ACATCTTTTA CTTTGCAGGA GAAACTCCAG AACAAGCAGA GGATTGGATG 
AAAGGTCTGC AAGCATTTTG CAGTTTACGG AAAAGTAGCC CAGGGACATC TAATAAACGC 
CTTCGTCAGG TCAGCAGCCT TGTTTTACAT ATTGAAGAAG CCCATAAACT ACCAGTAAAA 
CACTTTACTA ATCCATATTG TAACATCTAC TTGAATAGCG TCCAAGTAGC AAAAACTCAT 
GCAAGGGAGG GGCAAAACCC AGTATGGTCA GAGGAGTTTG TCTTTGATGA TCTTCCTCCT 
GACATCAATA GATTTGAAAT CACACTTAGT AATAAAACAA AGAAAAGCAA AGATCCAGAT 
ATCTTATTTA TGCGTTGCCA GTTGAGCAGG TTACAGAAAG GACATGCAAC AGATGAATGG 
TTTCTGCTAA GTTCCCATAT ACCACTAAAA GGTATTGAAC CAGGATCTTT GCGTGTTCGA 
GCACGATATT CCATGGAAAA AATCATGCCA GAAGAAGAGT ACAGTGAATT TAAAGAGCTT 
ATACTGCAAA AGGAACTTCA TGTCGTCTAT GCCTTATCAC ATGTATGTGG ACAAGACCGA 
ACACTACTGG CCAGCATCCT ACTAAAGATA TTTCTTCATG AAAAGCTTGA ATCATTGTTG 
TTATGCACAC TAAATGACAG AGAGATAAGT ATGGAAGATG AAGCCACTAC CCTATTTCGA 
GCTACAACAC TTGCAAGTAC CTTGATGGAG CAGTATATGA AAGCTACTGC CACACAATTT 
GTTCATCATG CTTTGAAAGA CTCCATTTTA AAGATTATGG AAAGCAAGCA ATCATGTGAG 
TTAAGTCCGT CAAAGTTAGA AAAAAATGAA GATGTGAACA CTAATTTAGC ACACCTATTG 
AGCATACTTT CAGAGCTTGT GGAGAAAATA TTCATGGCTT CAGAAATACT TCCACCGACA 
CTGAGATATA TCTATGGGTG TTTACAGAAA TCAGTTCAGC ATAAGTGGCC TACAAATAAC 
ACCATGAGAA CAAGAGTCGT TAGTGGTTTT GTTTTTCTAC GCCTTATCTG CCCTGCTATC 
CTGAATCCAC GGATGTTTAA TATCATCTCA GATTCCCCAT CTCCTATTGC TGCAAGAACA 
CTGACATTAG TGGCTAAATC TGTGCAGAAT TTAGCAAATC TTGTGGAATT TGGAGCTAAG 
GAGCCTTATA TGGAAGGTGT CAACCCATTT ATAAAAAGTA ATAAACATCG CATGATCATG 
TTTTTAGATG AACTTGGGAA TGTACCTGAA CTTCCGGACA CTACAGAGCA TTCTAGAACT 
GACCTGTCTC GTGACTTAGC AGCATTGCAT GAGATCTGTG TAGCTCATTC AGATGAACTG 
AGAACGCTCA GTAATGAGCG TGGTGTACAG CAGCATGTAC TGAAAAAGCT TCTGGCAATA 
ACAGAACTGC TTCAACAAAA ACAAAACCAG TACACAAAAA CCAATGACAT CAGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021036163.1
CDS	581..3697
Translation	MMAAEAGSEEGGPATAGAGGGAATGSSAYSAACRVKLPAALPVAAAPCPGLAEADLVAALGGGAASGSGFLGTGPVAGVLGGAALTGSAAAGVAGAAAAGPAGDVAFTKGTLSLPAETLGPGGGFPPLPPPPQLPPLGSGLGTVDEGDSLDGPEYEEEEVAIPLTAPPTNQWYHGKLDRTIAEERLRQAGKSGSYLIRESDRRPGSFVLSFLSQTNVVNHFRIIAMCGDYYIGGRRFSSLSDLIGYYSHVSCLLKGEKLLYPVAPPEPVEDRRRVRAILPYTKVPDTDEISFLKGDMFIVHNELEDGWMWVTNLRTDEQGLIVEDLVEEVSREEDPHEGKIWFHGKISKQEAYNLLMTVGQVCSFLVRPSDNTPGDYSLYFRTNENIQRFKICPTPNNQFMMGGRYYNSIGDIIDHYRKEQIVEGYYLKEPVPMQDQGQVLNDTVDGKEIYNTIRRKTKDAFYKNIVKKGYLLKKGKGKRWKNLYFILEGSDAQLIYFESEKRATKPKGLIDLSVCSVYVVHDSLFGRPNCFQIVVQHFSEEHYIFYFAGETPEQAEDWMKGLQAFCSLRKSSPGTSNKRLRQVSSLVLHIEEAHKLPVKHFTNPYCNIYLNSVQVAKTHAREGQNPVWSEEFVFDDLPPDINRFEITLSNKTKKSKDPDILFMRCQLSRLQKGHATDEWFLLSSHIPLKGIEPGSLRVRARYSMEKIMPEEEYSEFKELILQKELHVVYALSHVCGQDRTLLASILLKIFLHEKLESLLLCTLNDREISMEDEATTLFRATTLASTLMEQYMKATATQFVHHALKDSILKIMESKQSCELSPSKLEKNEDVNTNLAHLLSILSELVEKIFMASEILPPTLRYIYGCLQKSVQHKWPTNNTMRTRVVSGFVFLRLICPAILNPRMFNIISDSPSPIAARTLTLVAKSVQNLANLVEFGAKEPYMEGVNPFIKSNKHRMIMFLDELGNVPELPDTTEHSRTDLSRDLAALHEICVAHSDELRTLSNERGVQQHVLKKLLAITELLQQKQNQYTKTNDIR

Target ORF information:

RefSeq Version	XM_021180504.1
Organism	Mus caroli(Ryukyu mouse)
Definition	Mus caroli RAS p21 protein activator 1 (Rasa1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021180504.1

ORF Insert Sequence:

ATGATGGCGG CCGAGGCGGG CAGTGAGGAA GGCGGCCCTG CGACGGCCGG GGCCGGCGGC 
GGCGCGGCCA CGGGCTCCAG CGCCTACTCG GCAGCGTGCC GGGTCAAGTT ACCCGCGGCC 
CTGCCGGTGG CAGCCGCCCC TTGTCCCGGG CTGGCGGAGG CCGACTTGGT TGCGGCCCTC 
GGTGGCGGAG CCGCGTCGGG GTCCGGGTTC CTGGGGACCG GACCGGTGGC CGGTGTCTTG 
GGGGGCGCCG CGCTGACCGG CAGTGCTGCT GCTGGCGTGG CTGGTGCTGC GGCCGCCGGA 
CCTGCTGGAG ACGTCGCTTT CACCAAGGGG ACTCTGTCTT TGCCTGCTGA GACGCTCGGG 
CCGGGCGGCG GATTCCCTCC TCTGCCGCCG CCTCCTCAGC TGCCCCCTTT GGGCTCCGGT 
CTGGGGACCG TGGATGAAGG TGACTCCCTG GATGGACCAG AATACGAAGA GGAAGAGGTG 
GCCATACCGC TGACCGCACC TCCCACTAAC CAGTGGTATC ATGGAAAACT TGATAGAACT 
ATAGCAGAAG AACGCCTCAG GCAGGCAGGG AAATCTGGAA GCTATCTTAT AAGAGAGAGT 
GATCGGAGGC CGGGGTCCTT TGTCCTTTCA TTCCTTAGTC AGACAAATGT TGTCAATCAC 
TTTAGGATTA TTGCTATGTG TGGTGATTAC TACATTGGTG GAAGGCGATT TTCTTCACTC 
TCAGACCTAA TAGGTTATTA CAGTCATGTT TCTTGTTTAC TTAAAGGAGA AAAATTACTC 
TATCCAGTTG CACCACCAGA GCCAGTAGAA GATAGAAGGC GTGTGCGAGC CATTCTGCCC 
TACACAAAAG TACCAGACAC TGATGAAATA AGTTTCTTAA AAGGAGATAT GTTCATTGTT 
CACAATGAGT TAGAAGATGG ATGGATGTGG GTTACCAATC TAAGAACAGA TGAGCAAGGC 
CTTATTGTTG AAGACCTAGT GGAAGAGGTG AGCCGAGAAG AAGATCCACA TGAAGGCAAA 
ATATGGTTCC ACGGGAAAAT TTCCAAACAA GAAGCTTATA ATTTATTAAT GACAGTTGGA 
CAAGTCTGCA GCTTTCTTGT GAGGCCCTCA GATAATACTC CTGGAGATTA TTCCCTGTAT 
TTTCGGACCA ATGAAAATAT TCAGCGATTT AAAATTTGTC CAACACCTAA CAACCAGTTT 
ATGATGGGAG GCCGCTATTA TAACAGTATT GGGGACATCA TAGACCATTA TCGGAAAGAA 
CAAATTGTTG AAGGCTATTA TCTTAAGGAA CCTGTACCAA TGCAGGATCA AGGGCAAGTA 
CTCAATGACA CAGTGGATGG GAAGGAAATC TATAATACAA TTCGTCGTAA AACAAAGGAC 
GCTTTTTATA AAAATATTGT TAAGAAAGGT TATCTTCTGA AGAAGGGTAA AGGAAAACGT 
TGGAAAAATT TATATTTTAT CTTAGAAGGC AGTGATGCTC AACTTATTTA TTTTGAAAGT 
GAAAAACGAG CTACAAAACC AAAAGGGCTA ATAGATCTTA GTGTGTGTTC TGTCTATGTT 
GTCCATGACA GTCTCTTCGG CAGGCCAAAC TGTTTTCAAA TAGTAGTTCA GCACTTTAGT 
GAAGAACATT ACATCTTTTA CTTTGCAGGA GAAACTCCAG AACAAGCAGA GGATTGGATG 
AAAGGTCTGC AAGCATTTTG CAGTTTACGG AAAAGTAGCC CAGGGACATC TAATAAACGC 
CTTCGTCAGG TCAGCAGCCT TGTTTTACAT ATTGAAGAAG CCCATAAACT ACCAGTAAAA 
CACTTTACTA ATCCATATTG TAACATCTAC TTGAATAGCG TCCAAGTAGC AAAAACTCAT 
GCAAGGGAGG GGCAAAACCC AGTATGGTCA GAGGAGTTTG TCTTTGATGA TCTTCCTCCT 
GACATCAATA GATTTGAAAT CACACTTAGT AATAAAACAA AGAAAAGCAA AGATCCAGAT 
ATCTTATTTA TGCGTTGCCA GTTGAGCAGG TTACAGAAAG GACATGCAAC AGATGAATGG 
TTTCTGCTAA GTTCCCATAT ACCACTAAAA GGTATTGAAC CAGGATCTTT GCGTGTTCGA 
GCACGATATT CCATGGAAAA AATCATGCCA GAAGAAGAGT ACAGTGAATT TAAAGAGCTT 
ATACTGCAAA AGGAACTTCA TGTCGTCTAT GCCTTATCAC ATGTATGTGG ACAAGACCGA 
ACACTACTGG CCAGCATCCT ACTAAAGATA TTTCTTCATG AAAAGCTTGA ATCATTGTTG 
TTATGCACAC TAAATGACAG AGAGATAAGT ATGGAAGATG AAGCCACTAC CCTATTTCGA 
GCTACAACAC TTGCAAGTAC CTTGATGGAG CAGTATATGA AAGCTACTGC CACACAATTT 
GTTCATCATG CTTTGAAAGA CTCCATTTTA AAGATTATGG AAAGCAAGCA ATCATGTGAG 
TTAAGTCCGT CAAAGTTAGA AAAAAATGAA GATGTGAACA CTAATTTAGC ACACCTATTG 
AGCATACTTT CAGAGCTTGT GGAGAAAATA TTCATGGCTT CAGAAATACT TCCACCGACA 
CTGAGATATA TCTATGGGTG TTTACAGAAA TCAGTTCAGC ATAAGTGGCC TACAAATAAC 
ACCATGAGAA CAAGAGTCGT TAGTGGTTTT GTTTTTCTAC GCCTTATCTG CCCTGCTATC 
CTGAATCCAC GGATGTTTAA TATCATCTCA GATTCCCCAT CTCCTATTGC TGCAAGAACA 
CTGACATTAG TGGCTAAATC TGTGCAGAAT TTAGCAAATC TTGTGGAATT TGGAGCTAAG 
GAGCCTTATA TGGAAGGTGT CAACCCATTT ATAAAAAGTA ATAAACATCG CATGATCATG 
TTTTTAGATG AACTTGGGAA TGTACCTGAA CTTCCGGACA CTACAGAGCA TTCTAGAACT 
GACCTGTCTC GTGACTTAGC AGCATTGCAT GAGATCTGTG TAGCTCATTC AGATGAACTG 
AGAACGCTCA GTAATGAGCG TGGTGTACAG CAGCATGTAC TGAAAAAGCT TCTGGCAATA 
ACAGAACTGC TTCAACAAAA ACAAAACCAG TACACAAAAA CCAATGACAT CAGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Rasa1 ( XM_021180504.2 ) cDNA ORF clone, Mus caroli(Ryukyu mouse) -> XP_021036163.1 Mus caroli RAS p21 protein activator 1 (Rasa1), mRNA.

Price & Availability↑

CloneID	OMv29923
Clone ID Related Accession (Same CDS sequence)	XM_021180504.1 , XM_021180504.2
Accession Version	XM_021180504.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3117bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-06-09
Organism	Mus caroli(Ryukyu mouse)
Product	ras GTPase-activating protein 1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_034582.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process On Jun 10, 2019 this sequence version replaced XM_021180504.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus caroli Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGGCGG CCGAGGCGGG CAGTGAGGAA GGCGGCCCTG CGACGGCCGG GGCCGGCGGC 
GGCGCGGCCA CGGGCTCCAG CGCCTACTCG GCAGCGTGCC GGGTCAAGTT ACCCGCGGCC 
CTGCCGGTGG CAGCCGCCCC TTGTCCCGGG CTGGCGGAGG CCGACTTGGT TGCGGCCCTC 
GGTGGCGGAG CCGCGTCGGG GTCCGGGTTC CTGGGGACCG GACCGGTGGC CGGTGTCTTG 
GGGGGCGCCG CGCTGACCGG CAGTGCTGCT GCTGGCGTGG CTGGTGCTGC GGCCGCCGGA 
CCTGCTGGAG ACGTCGCTTT CACCAAGGGG ACTCTGTCTT TGCCTGCTGA GACGCTCGGG 
CCGGGCGGCG GATTCCCTCC TCTGCCGCCG CCTCCTCAGC TGCCCCCTTT GGGCTCCGGT 
CTGGGGACCG TGGATGAAGG TGACTCCCTG GATGGACCAG AATACGAAGA GGAAGAGGTG 
GCCATACCGC TGACCGCACC TCCCACTAAC CAGTGGTATC ATGGAAAACT TGATAGAACT 
ATAGCAGAAG AACGCCTCAG GCAGGCAGGG AAATCTGGAA GCTATCTTAT AAGAGAGAGT 
GATCGGAGGC CGGGGTCCTT TGTCCTTTCA TTCCTTAGTC AGACAAATGT TGTCAATCAC 
TTTAGGATTA TTGCTATGTG TGGTGATTAC TACATTGGTG GAAGGCGATT TTCTTCACTC 
TCAGACCTAA TAGGTTATTA CAGTCATGTT TCTTGTTTAC TTAAAGGAGA AAAATTACTC 
TATCCAGTTG CACCACCAGA GCCAGTAGAA GATAGAAGGC GTGTGCGAGC CATTCTGCCC 
TACACAAAAG TACCAGACAC TGATGAAATA AGTTTCTTAA AAGGAGATAT GTTCATTGTT 
CACAATGAGT TAGAAGATGG ATGGATGTGG GTTACCAATC TAAGAACAGA TGAGCAAGGC 
CTTATTGTTG AAGACCTAGT GGAAGAGGTG AGCCGAGAAG AAGATCCACA TGAAGGCAAA 
ATATGGTTCC ACGGGAAAAT TTCCAAACAA GAAGCTTATA ATTTATTAAT GACAGTTGGA 
CAAGTCTGCA GCTTTCTTGT GAGGCCCTCA GATAATACTC CTGGAGATTA TTCCCTGTAT 
TTTCGGACCA ATGAAAATAT TCAGCGATTT AAAATTTGTC CAACACCTAA CAACCAGTTT 
ATGATGGGAG GCCGCTATTA TAACAGTATT GGGGACATCA TAGACCATTA TCGGAAAGAA 
CAAATTGTTG AAGGCTATTA TCTTAAGGAA CCTGTACCAA TGCAGGATCA AGGGCAAGTA 
CTCAATGACA CAGTGGATGG GAAGGAAATC TATAATACAA TTCGTCGTAA AACAAAGGAC 
GCTTTTTATA AAAATATTGT TAAGAAAGGT TATCTTCTGA AGAAGGGTAA AGGAAAACGT 
TGGAAAAATT TATATTTTAT CTTAGAAGGC AGTGATGCTC AACTTATTTA TTTTGAAAGT 
GAAAAACGAG CTACAAAACC AAAAGGGCTA ATAGATCTTA GTGTGTGTTC TGTCTATGTT 
GTCCATGACA GTCTCTTCGG CAGGCCAAAC TGTTTTCAAA TAGTAGTTCA GCACTTTAGT 
GAAGAACATT ACATCTTTTA CTTTGCAGGA GAAACTCCAG AACAAGCAGA GGATTGGATG 
AAAGGTCTGC AAGCATTTTG CAGTTTACGG AAAAGTAGCC CAGGGACATC TAATAAACGC 
CTTCGTCAGG TCAGCAGCCT TGTTTTACAT ATTGAAGAAG CCCATAAACT ACCAGTAAAA 
CACTTTACTA ATCCATATTG TAACATCTAC TTGAATAGCG TCCAAGTAGC AAAAACTCAT 
GCAAGGGAGG GGCAAAACCC AGTATGGTCA GAGGAGTTTG TCTTTGATGA TCTTCCTCCT 
GACATCAATA GATTTGAAAT CACACTTAGT AATAAAACAA AGAAAAGCAA AGATCCAGAT 
ATCTTATTTA TGCGTTGCCA GTTGAGCAGG TTACAGAAAG GACATGCAAC AGATGAATGG 
TTTCTGCTAA GTTCCCATAT ACCACTAAAA GGTATTGAAC CAGGATCTTT GCGTGTTCGA 
GCACGATATT CCATGGAAAA AATCATGCCA GAAGAAGAGT ACAGTGAATT TAAAGAGCTT 
ATACTGCAAA AGGAACTTCA TGTCGTCTAT GCCTTATCAC ATGTATGTGG ACAAGACCGA 
ACACTACTGG CCAGCATCCT ACTAAAGATA TTTCTTCATG AAAAGCTTGA ATCATTGTTG 
TTATGCACAC TAAATGACAG AGAGATAAGT ATGGAAGATG AAGCCACTAC CCTATTTCGA 
GCTACAACAC TTGCAAGTAC CTTGATGGAG CAGTATATGA AAGCTACTGC CACACAATTT 
GTTCATCATG CTTTGAAAGA CTCCATTTTA AAGATTATGG AAAGCAAGCA ATCATGTGAG 
TTAAGTCCGT CAAAGTTAGA AAAAAATGAA GATGTGAACA CTAATTTAGC ACACCTATTG 
AGCATACTTT CAGAGCTTGT GGAGAAAATA TTCATGGCTT CAGAAATACT TCCACCGACA 
CTGAGATATA TCTATGGGTG TTTACAGAAA TCAGTTCAGC ATAAGTGGCC TACAAATAAC 
ACCATGAGAA CAAGAGTCGT TAGTGGTTTT GTTTTTCTAC GCCTTATCTG CCCTGCTATC 
CTGAATCCAC GGATGTTTAA TATCATCTCA GATTCCCCAT CTCCTATTGC TGCAAGAACA 
CTGACATTAG TGGCTAAATC TGTGCAGAAT TTAGCAAATC TTGTGGAATT TGGAGCTAAG 
GAGCCTTATA TGGAAGGTGT CAACCCATTT ATAAAAAGTA ATAAACATCG CATGATCATG 
TTTTTAGATG AACTTGGGAA TGTACCTGAA CTTCCGGACA CTACAGAGCA TTCTAGAACT 
GACCTGTCTC GTGACTTAGC AGCATTGCAT GAGATCTGTG TAGCTCATTC AGATGAACTG 
AGAACGCTCA GTAATGAGCG TGGTGTACAG CAGCATGTAC TGAAAAAGCT TCTGGCAATA 
ACAGAACTGC TTCAACAAAA ACAAAACCAG TACACAAAAA CCAATGACAT CAGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021036163.1
CDS	581..3697
Translation	MMAAEAGSEEGGPATAGAGGGAATGSSAYSAACRVKLPAALPVAAAPCPGLAEADLVAALGGGAASGSGFLGTGPVAGVLGGAALTGSAAAGVAGAAAAGPAGDVAFTKGTLSLPAETLGPGGGFPPLPPPPQLPPLGSGLGTVDEGDSLDGPEYEEEEVAIPLTAPPTNQWYHGKLDRTIAEERLRQAGKSGSYLIRESDRRPGSFVLSFLSQTNVVNHFRIIAMCGDYYIGGRRFSSLSDLIGYYSHVSCLLKGEKLLYPVAPPEPVEDRRRVRAILPYTKVPDTDEISFLKGDMFIVHNELEDGWMWVTNLRTDEQGLIVEDLVEEVSREEDPHEGKIWFHGKISKQEAYNLLMTVGQVCSFLVRPSDNTPGDYSLYFRTNENIQRFKICPTPNNQFMMGGRYYNSIGDIIDHYRKEQIVEGYYLKEPVPMQDQGQVLNDTVDGKEIYNTIRRKTKDAFYKNIVKKGYLLKKGKGKRWKNLYFILEGSDAQLIYFESEKRATKPKGLIDLSVCSVYVVHDSLFGRPNCFQIVVQHFSEEHYIFYFAGETPEQAEDWMKGLQAFCSLRKSSPGTSNKRLRQVSSLVLHIEEAHKLPVKHFTNPYCNIYLNSVQVAKTHAREGQNPVWSEEFVFDDLPPDINRFEITLSNKTKKSKDPDILFMRCQLSRLQKGHATDEWFLLSSHIPLKGIEPGSLRVRARYSMEKIMPEEEYSEFKELILQKELHVVYALSHVCGQDRTLLASILLKIFLHEKLESLLLCTLNDREISMEDEATTLFRATTLASTLMEQYMKATATQFVHHALKDSILKIMESKQSCELSPSKLEKNEDVNTNLAHLLSILSELVEKIFMASEILPPTLRYIYGCLQKSVQHKWPTNNTMRTRVVSGFVFLRLICPAILNPRMFNIISDSPSPIAARTLTLVAKSVQNLANLVEFGAKEPYMEGVNPFIKSNKHRMIMFLDELGNVPELPDTTEHSRTDLSRDLAALHEICVAHSDELRTLSNERGVQQHVLKKLLAITELLQQKQNQYTKTNDIR

Target ORF information:

RefSeq Version	XM_021180504.2
Organism	Mus caroli(Ryukyu mouse)
Definition	Mus caroli RAS p21 protein activator 1 (Rasa1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021180504.2

ORF Insert Sequence:

ATGATGGCGG CCGAGGCGGG CAGTGAGGAA GGCGGCCCTG CGACGGCCGG GGCCGGCGGC 
GGCGCGGCCA CGGGCTCCAG CGCCTACTCG GCAGCGTGCC GGGTCAAGTT ACCCGCGGCC 
CTGCCGGTGG CAGCCGCCCC TTGTCCCGGG CTGGCGGAGG CCGACTTGGT TGCGGCCCTC 
GGTGGCGGAG CCGCGTCGGG GTCCGGGTTC CTGGGGACCG GACCGGTGGC CGGTGTCTTG 
GGGGGCGCCG CGCTGACCGG CAGTGCTGCT GCTGGCGTGG CTGGTGCTGC GGCCGCCGGA 
CCTGCTGGAG ACGTCGCTTT CACCAAGGGG ACTCTGTCTT TGCCTGCTGA GACGCTCGGG 
CCGGGCGGCG GATTCCCTCC TCTGCCGCCG CCTCCTCAGC TGCCCCCTTT GGGCTCCGGT 
CTGGGGACCG TGGATGAAGG TGACTCCCTG GATGGACCAG AATACGAAGA GGAAGAGGTG 
GCCATACCGC TGACCGCACC TCCCACTAAC CAGTGGTATC ATGGAAAACT TGATAGAACT 
ATAGCAGAAG AACGCCTCAG GCAGGCAGGG AAATCTGGAA GCTATCTTAT AAGAGAGAGT 
GATCGGAGGC CGGGGTCCTT TGTCCTTTCA TTCCTTAGTC AGACAAATGT TGTCAATCAC 
TTTAGGATTA TTGCTATGTG TGGTGATTAC TACATTGGTG GAAGGCGATT TTCTTCACTC 
TCAGACCTAA TAGGTTATTA CAGTCATGTT TCTTGTTTAC TTAAAGGAGA AAAATTACTC 
TATCCAGTTG CACCACCAGA GCCAGTAGAA GATAGAAGGC GTGTGCGAGC CATTCTGCCC 
TACACAAAAG TACCAGACAC TGATGAAATA AGTTTCTTAA AAGGAGATAT GTTCATTGTT 
CACAATGAGT TAGAAGATGG ATGGATGTGG GTTACCAATC TAAGAACAGA TGAGCAAGGC 
CTTATTGTTG AAGACCTAGT GGAAGAGGTG AGCCGAGAAG AAGATCCACA TGAAGGCAAA 
ATATGGTTCC ACGGGAAAAT TTCCAAACAA GAAGCTTATA ATTTATTAAT GACAGTTGGA 
CAAGTCTGCA GCTTTCTTGT GAGGCCCTCA GATAATACTC CTGGAGATTA TTCCCTGTAT 
TTTCGGACCA ATGAAAATAT TCAGCGATTT AAAATTTGTC CAACACCTAA CAACCAGTTT 
ATGATGGGAG GCCGCTATTA TAACAGTATT GGGGACATCA TAGACCATTA TCGGAAAGAA 
CAAATTGTTG AAGGCTATTA TCTTAAGGAA CCTGTACCAA TGCAGGATCA AGGGCAAGTA 
CTCAATGACA CAGTGGATGG GAAGGAAATC TATAATACAA TTCGTCGTAA AACAAAGGAC 
GCTTTTTATA AAAATATTGT TAAGAAAGGT TATCTTCTGA AGAAGGGTAA AGGAAAACGT 
TGGAAAAATT TATATTTTAT CTTAGAAGGC AGTGATGCTC AACTTATTTA TTTTGAAAGT 
GAAAAACGAG CTACAAAACC AAAAGGGCTA ATAGATCTTA GTGTGTGTTC TGTCTATGTT 
GTCCATGACA GTCTCTTCGG CAGGCCAAAC TGTTTTCAAA TAGTAGTTCA GCACTTTAGT 
GAAGAACATT ACATCTTTTA CTTTGCAGGA GAAACTCCAG AACAAGCAGA GGATTGGATG 
AAAGGTCTGC AAGCATTTTG CAGTTTACGG AAAAGTAGCC CAGGGACATC TAATAAACGC 
CTTCGTCAGG TCAGCAGCCT TGTTTTACAT ATTGAAGAAG CCCATAAACT ACCAGTAAAA 
CACTTTACTA ATCCATATTG TAACATCTAC TTGAATAGCG TCCAAGTAGC AAAAACTCAT 
GCAAGGGAGG GGCAAAACCC AGTATGGTCA GAGGAGTTTG TCTTTGATGA TCTTCCTCCT 
GACATCAATA GATTTGAAAT CACACTTAGT AATAAAACAA AGAAAAGCAA AGATCCAGAT 
ATCTTATTTA TGCGTTGCCA GTTGAGCAGG TTACAGAAAG GACATGCAAC AGATGAATGG 
TTTCTGCTAA GTTCCCATAT ACCACTAAAA GGTATTGAAC CAGGATCTTT GCGTGTTCGA 
GCACGATATT CCATGGAAAA AATCATGCCA GAAGAAGAGT ACAGTGAATT TAAAGAGCTT 
ATACTGCAAA AGGAACTTCA TGTCGTCTAT GCCTTATCAC ATGTATGTGG ACAAGACCGA 
ACACTACTGG CCAGCATCCT ACTAAAGATA TTTCTTCATG AAAAGCTTGA ATCATTGTTG 
TTATGCACAC TAAATGACAG AGAGATAAGT ATGGAAGATG AAGCCACTAC CCTATTTCGA 
GCTACAACAC TTGCAAGTAC CTTGATGGAG CAGTATATGA AAGCTACTGC CACACAATTT 
GTTCATCATG CTTTGAAAGA CTCCATTTTA AAGATTATGG AAAGCAAGCA ATCATGTGAG 
TTAAGTCCGT CAAAGTTAGA AAAAAATGAA GATGTGAACA CTAATTTAGC ACACCTATTG 
AGCATACTTT CAGAGCTTGT GGAGAAAATA TTCATGGCTT CAGAAATACT TCCACCGACA 
CTGAGATATA TCTATGGGTG TTTACAGAAA TCAGTTCAGC ATAAGTGGCC TACAAATAAC 
ACCATGAGAA CAAGAGTCGT TAGTGGTTTT GTTTTTCTAC GCCTTATCTG CCCTGCTATC 
CTGAATCCAC GGATGTTTAA TATCATCTCA GATTCCCCAT CTCCTATTGC TGCAAGAACA 
CTGACATTAG TGGCTAAATC TGTGCAGAAT TTAGCAAATC TTGTGGAATT TGGAGCTAAG 
GAGCCTTATA TGGAAGGTGT CAACCCATTT ATAAAAAGTA ATAAACATCG CATGATCATG 
TTTTTAGATG AACTTGGGAA TGTACCTGAA CTTCCGGACA CTACAGAGCA TTCTAGAACT 
GACCTGTCTC GTGACTTAGC AGCATTGCAT GAGATCTGTG TAGCTCATTC AGATGAACTG 
AGAACGCTCA GTAATGAGCG TGGTGTACAG CAGCATGTAC TGAAAAAGCT TCTGGCAATA 
ACAGAACTGC TTCAACAAAA ACAAAACCAG TACACAAAAA CCAATGACAT CAGGTAG