Tjp1 gene cDNA ORF clone, Mus caroli(Ryukyu mouse)

mRNA	Protein	Name
XM_021166165.1	XP_021021824.1	tight junction protein ZO-1 isoform X2
XM_021166166.1	XP_021021825.1	tight junction protein ZO-1 isoform X3
XM_029479116.1	XP_029334976.1	tight junction protein ZO-1 isoform X1
XM_021166166.2	XP_021021825.1	tight junction protein ZO-1 isoform X9
XM_021166164.1	XP_021021823.1	tight junction protein ZO-1 isoform X1
XM_029479117.1	XP_029334977.1	tight junction protein ZO-1 isoform X2
XM_029479118.1	XP_029334978.1	tight junction protein ZO-1 isoform X3
XM_029479120.1	XP_029334980.1	tight junction protein ZO-1 isoform X5
XM_021166167.1	XP_021021826.1	tight junction protein ZO-1 isoform X4
XM_021166164.2	XP_021021823.1	tight junction protein ZO-1 isoform X6
XM_029479121.1	XP_029334981.1	tight junction protein ZO-1 isoform X7
XM_021166167.2	XP_021021826.1	tight junction protein ZO-1 isoform X10
XM_029479119.1	XP_029334979.1	tight junction protein ZO-1 isoform X4
XM_021166165.2	XP_021021824.1	tight junction protein ZO-1 isoform X8

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMv03978	XM_021166164.2 Latest version!	Mus caroli tight junction protein 1 (Tjp1), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OMv03979	XM_021166165.2 Latest version!	Mus caroli tight junction protein 1 (Tjp1), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OMv03980	XM_021166166.2 Latest version!	Mus caroli tight junction protein 1 (Tjp1), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OMv03981	XM_021166167.2 Latest version!	Mus caroli tight junction protein 1 (Tjp1), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
OMv74613	XM_029479116.1 Latest version!	Mus caroli tight junction protein 1 (Tjp1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OMv74614	XM_029479117.1 Latest version!	Mus caroli tight junction protein 1 (Tjp1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OMv74615	XM_029479118.1 Latest version!	Mus caroli tight junction protein 1 (Tjp1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OMv74616	XM_029479119.1 Latest version!	Mus caroli tight junction protein 1 (Tjp1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OMv74617	XM_029479120.1 Latest version!	Mus caroli tight junction protein 1 (Tjp1), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OMv74618	XM_029479121.1 Latest version!	Mus caroli tight junction protein 1 (Tjp1), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
View Old Accession Versions >>
OMv03980	XM_021166166.1	Mus caroli tight junction protein 1 (Tjp1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OMv03978	XM_021166164.1	Mus caroli tight junction protein 1 (Tjp1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OMv03981	XM_021166167.1	Mus caroli tight junction protein 1 (Tjp1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
OMv03979	XM_021166165.1	Mus caroli tight junction protein 1 (Tjp1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
Hide Old Accession Versions >>

Tjp1 ( XM_021166164.1 ) cDNA ORF clone, Mus caroli(Ryukyu mouse) -> XP_021021823.1 Mus caroli tight junction protein 1 (Tjp1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMv03978
Clone ID Related Accession (Same CDS sequence)	XM_021166164.1 , XM_021166164.2
Accession Version	XM_021166164.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5298bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-05-20
Organism	Mus caroli(Ryukyu mouse)
Product	tight junction protein ZO-1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_034576.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus caroli Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCACCA GGGCCGCGGC CGCTAAGAGC ACAGCAATGG AGGAAACGGC TATATGGGAA 
CAGCACACAG TGATGCTTCA CAGGGCTCCT GGGTTTGGAT TTGGAATTGC AATCTCTGGT 
GGAAGAGATA ATCCTCATTT TCAGAGTGGG GAAACCTCCA TAGTGATTTC TGATGTGCTA 
AAAGGAGGGC CAGCTGAAGG ACAGCTACAG GAAAATGACC GAGTTGCAAT GGTTAATGGA 
GTTTCAATGG ATAACGTTGA ACATGCTTTT GCTGTTCAGC AGCTAAGGAA AAGTGGGAAA 
AACGCAAAAA TTACTATGCG AAGGAAGAAG AAAGTTCAGA TCCCTGTAAG TCACCCAGAT 
CCTGAGCCGG TGTCTGATAA TGAAGATGAT AGCTACGACG AAGAAGTACA TGATCCAAGA 
GCTGGCCGCA GTGCTTTAGC GAACAGAAGG AGCGAGAAGA GCTGGGCAAG GGATAGGAGT 
GCAAGCAGGG AGAGGAGCCT GTCCCCTCGC TCGGACAGGC GGTCGGTGGC CTCCAGTCAG 
CCCGCAAAAC CCACCAAGGT CGCACTGGTG AAGTCTCGGA AAAATGAAGA ATATGGTCTT 
CGATTGGCCA GCCATATATT TGTAAAGGAA ATTTCACAAG ATAGTTTGGC AGCAAGAGAT 
GGTAACATTC AAGAAGGGGA TGTTGTCTTG AAGATAAATG GTACTGTGAC AGAAAATATG 
TCATTGACAG ATGCAAAAAC ACTGATAGAA AGGTCTAAAG GCAAGTTAAA AATGGTAGTG 
CAAAGAGATG AGCGGGCTAC CTTACTGAAC GTCCCTGACC TTTCGGATAG TATCCATTCT 
GCTAATGCCT CGGAAAGAGA TGACATTTCA GAAATTCAGT CACTAGCATC AGACCATTCA 
GGTCGCTCGC ATGACAGGCC ACCCCGCCGC AGCCAGTCAC GATCTCCTGA CCAACGTTCA 
GAGCCCTCCG ATCATTCCAC ACAGTCTCCA CAGCAGCCCA GCAATGGCAG TCTCCGGAGC 
AGAGAGCAAG AGCGAATGTC TAAACCTGGG GCCATCTCAA CTCCTGTAAA ACATGCAGAT 
GATCATACAC CCAAAGCAGT GGAAGAAGTT ACAGTTGAGA AAAATGAGAA GCAGACACCC 
ACTCTTCCAG AACCGAAGCC TGTATATGCT CAAGTTGGAC AACCAGATGT GGATTTACCC 
GTCAGCCCTT CTGATGGTGT TCTGCCTAAT TCAACTCATG AAGATGGGAT ACTTAGGCCC 
AGCATGAAAC TGGTAAAATT CAGAAAAGGA GATAGTGTGG GTTTGCGACT AGCTGGTGGA 
AATGATGTCG GAATATTTGT AGCTGGCGTT CTAGAAGATA GCCCTGCAGC CAAAGAAGGC 
TTAGAGGAAG GTGATCAAAT TCTCAGGGTG AACAATGTAG ATTTCACAAA TATCATAAGA 
GAAGAGGCTG TCCTTTTCCT CCTTGACCTC CCTAAAGGTG AAGAAGTGAC CATCCTGGCT 
CAGAAGAAGA AGGGTGTTTA TCGCCGCATT GTAGAATCAG ATGTAGGAGA TTCATTCTAT 
ATTAGAACGC ATTTTGAGTA TGAAAAAGAA TCTCCTTACG GACTTAGTTT TAACAAAGGA 
GAGGTGTTCC GGGTCGTGGA TACTTTATAC AATGGAAAGC TGGGCTCTTG GCTTGCCATT 
CGAATTGGCA AAAATCATAA GGAGGTAGAA CGAGGCATCA TCCCTAATAA GAACAGAGCT 
GAGCAGTTAG CCAGTGTACA GTACACACTT CCAAAGACAG CGGGTGGTGA TCGGGCAGAC 
TTCTGGAGGT TTCGAGGTCT TCGCAGCTCC AAGAGAAATC TTCGAAAAAG CAGAGAGGAC 
TTGTCAGCTC AGCCAGTTCA AACTAAGTTC CCAGCTTATG AAAGGGTTGT TCTTCGAGAA 
GCTGGATTCC TAAGACCTGT AACCATCTTT GGACCAATAG CTGATGTTGC CAGAGAAAAG 
TTGGCAAGAG AGGAACCAGA TATCTATCAG ATTGCAAAAA GTGAACCACG AGATGCTGGG 
ACTGACCATC GTAGCTCTGG CATCATTCGC CTTCATACAA TAAAGCAAAT CATAGATCAG 
GATAAACATG CGTTATTAGA TGTAACGCCA AATGCGGTTG ATCGTCTTAA TTATGCGCAG 
TGGTATCCAA TTGTTGTGTT CCTTAACCCT GACTCTAAGC AAGGTGTAAA AACAATGAGG 
ATGAGGTTAT GTCCGGAGTC TCGGAAAAGC GCCAGGAAGC TATATGAACG CTCTCATAAG 
CTTCGCAAAA ACAATCACCA CCTTTTCACA ACTACAATTA ACTTAAACTC AATGAATGAT 
GGTTGGTACG GTGCCCTGAA AGAAGCCATT CAGCAGCAAC AGAACCAGCT GGTGTGGGTC 
TCTGAGGGGA AGGCGGATGG TGCTACAAGT GATGACCTTG ATTTGCATGA TGATCGTCTG 
TCCTACCTGT CAGCCCCAGG TAGTGAGTAC TCAATGTATA GCACGGACAG TAGACACACT 
TCTGACTATG AAGACACAGA TACAGAAGGC GGGGCCTACA CTGATCAAGA ACTAGATGAA 
ACTCTTAATG ATGAGGTGGG GACTCCCCCG GAGTCTGCCA TTACACNGTC CTCTGAGCCT 
GTAAGAGAGG ATTCCTCTGG GATGCATCAT GAAAACCAGA CCTATCCTCC TTACTCACCA 
CAAGCGCAGC CACAAGCTAT TCATAGACTA GACTCCCCTG GACTTAAGCC AGCCTCTCAA 
CAGAAAGCAG AAGCCTCATC TCCAGTCCCT TACCTTTCGC CTGAAACAAA CCCAGCATCG 
TCAGCCTCTG CAGTTAATCC TAATGTCAGT GTAACTAATG TCAGCCTGGA GGAGCCTGCC 
CCAGCCCCTC CCGCCTCGCA CGCATCCCAG GCTGGTGGTT TAGGAGCACC AGGTGCTGAG 
GCAGCTCACG TAGTGCTCAG AGGTGAAGGA CCACCATTGC CGCCGCATGC AGACCCAGCA 
CAGGTGTATA GAAAGGAGCC GTATCCTGAA GAAATGATGA GACAAAACCA TATTTTAAAA 
CAACCAGCTC TTGGTCACCC AGGGCAGAGG CCAGATAAAG AGCCAAATCT AGCCTATGAA 
CCCCAACTTC CATATANAGA AAAACAAGCC AGCAGAGACC TTGAACAGCC GTCATACAGG 
TATGAGGCCT CAAGCTACCC AGACCAGTTT TCTCGGAACT ATGACCATCG CCTACGGTTT 
GAAGATCGAA TCCCTACCTA TGAAGACCAG TGGTCATATT ATGATGACAA ACAGCCCTAC 
CAACCTCGGC CTTTTGAGAA TCAGCATCCC CGAGACCTGG ACTCCAGACA ACATCCNGAG 
GAGGCTTCAG AACGAGGTTA TNTCCAGCGT TTTGAAGAGC CAGCCCCTCT GTCATACGAC 
AGTAGAACAC GCTATGAGCA GCTGCCTCGA ACCTCTACTC TACGACATGA AGAGCAGCCA 
GCCCCTGCAT ATGAGGTGCA CAACAGGTAC AGGCCAGAGG CACAGCCCTA TTCTTCAACA 
GGTCCTAAGT CATCTGAGCC CAAGCAGTAC TTTGACCAGT ACCCGCGAAG TTATGAGCAA 
GTACCACCAC CAGGCTTTAC CTCCAAAACA GGCCATTACG AGCCTCTCCA TGGTGCTGCA 
GTTGTCCCTC CTCTGATACC TTCCTCTCAA CATAAGCCAG AAGTCCTGCC CTCGGCTACC 
AAACCACAGC CTCCACCCCC AACCCTAACT GAGGAGGAGG AGGATCCAGC AATGAAACCA 
CAGTCCGTGC TCANCAGAGT CAAAATGTTT GAAAACAAAA GATCTGCGTC TTTGGAGAAC 
AAGAAAGATG TGAATGACAC TGCCAGCTTT AAGCCTCCAG AAGTAGCATC TAAACCTCCA 
AGTGCTTCTC TTGCTGGCCC TAAACCTGTT CCTCAGAGTC AGTTTAGTGA GCANGACAAA 
ACACTCTACA GGCTCCCAGA GCCTCAGAAA CCTCAAGTGA AGCCACCCGA NGATATTGTT 
CGATCAAATC ATTACGACCC TGAAGAGGAT GAAGAATATT ACAGGAAACA GCTCTCTTAC 
TTTGANCGAA GAAGTTTTGA GAGCAAGCCT TCTGCACATC TTCCTGCTGG CCATCACTCA 
GAGCCTGCTA AGCCAGTCCA TTCTCAGAGC CAGCCCAACT TTTCTAGTTA CTCTTCAAAG 
GGAAAACCCG AAACTGATGC TGTGGATAGA TCATTCAGTG AGAAACGTTA TGATCCAGCC 
CAGGCCACGC CTCCTCCTCC TCCGTTGCCC TCACAGTACA GCCAGCCAGC TCCCCCTCTG 
TCCAGCTCTT CTCTCCACAT ACATTCCAAG GGCGCCCAGG GCGAAGGCAA TTCAGTATCA 
TTGGATTTTC AGAACTCATA TATGTCCAAA CCAGACCCAC CCCCATCTCA GAGTAAACCA 
GCAACTTTCA GACCACCAAC CCGAGAAGAC CCTCCTCAGA CTTTCTATCC CCAGAAANGT 
TTCCCAGACA AAGCTCCAGT TAANGGAGCT GAGCAGACTC AGAAAACCAT CACTCCAGTG 
TACAATCGAT TCACACCAAA GCCGTACACA AGTTCTGCCC GGCCGTTTGA ACGCAAATTT 
GAAAGTCCGA AGTTCAACCA TAATCTTCTG CCAAGTGAAA CTGTACATAA ACCTGAATTG 
TCTTCAAAAA CTCCCACTTC TCCAAAAACT CTTATGAAAG CTCATAGTTC AACACAGCCT 
CCAGAGTTTG ACAGTGGAGT TGAAACTTTC TCTGTTCACA CAGATNAGCC TAAATATCAA 
ATGAATAATA TCAGCACCAT GCCTAAAGCT GTCCCTGTGA GTCCTTCAGC TGTGGAAGAA 
GATGAAGATG AGGATGGTCA TACTGTAGTG GCTACAGCTC GTGGCATTTT TAACAGCAAT 
GGTGGTGTGT TGAGTTCCAT AGAAACCGGT GTTAGTATAA TTATCCCACA AGGAGCCATT 
CCTGAAGGAA TTGAACAAGA AATCTATTTC AAAGTCTGCA GAGACAATAG CATTCTCCCA 
CCTTTAGATA AAGAGAAAGG TGAAACTCTG CTAAGTCCCC TAGTGATGTG TGGGCCCCAT 
GGCCTCAAGT TCCTGAAGCC CGTGGAGCTG CGCTTGCCAC ACTGTGCGTC CATGACTCCT 
GACGGTTGGT CTTTTGCTCT AAAATCATCC GACTCCTCGT CGGGTGACCC TAAAACCTGG 
CAAAACAAGT GTCTTCCTGG AGATCCGAAT TACCTTGTTG GAGCCAACTG TGTTTCTGTC 
CTGATTGACC ACTTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021021823.1
CDS	38..5335
Translation	MSTRAAAAKSTAMEETAIWEQHTVMLHRAPGFGFGIAISGGRDNPHFQSGETSIVISDVLKGGPAEGQLQENDRVAMVNGVSMDNVEHAFAVQQLRKSGKNAKITMRRKKKVQIPVSHPDPEPVSDNEDDSYDEEVHDPRAGRSALANRRSEKSWARDRSASRERSLSPRSDRRSVASSQPAKPTKVALVKSRKNEEYGLRLASHIFVKEISQDSLAARDGNIQEGDVVLKINGTVTENMSLTDAKTLIERSKGKLKMVVQRDERATLLNVPDLSDSIHSANASERDDISEIQSLASDHSGRSHDRPPRRSQSRSPDQRSEPSDHSTQSPQQPSNGSLRSREQERMSKPGAISTPVKHADDHTPKAVEEVTVEKNEKQTPTLPEPKPVYAQVGQPDVDLPVSPSDGVLPNSTHEDGILRPSMKLVKFRKGDSVGLRLAGGNDVGIFVAGVLEDSPAAKEGLEEGDQILRVNNVDFTNIIREEAVLFLLDLPKGEEVTILAQKKKGVYRRIVESDVGDSFYIRTHFEYEKESPYGLSFNKGEVFRVVDTLYNGKLGSWLAIRIGKNHKEVERGIIPNKNRAEQLASVQYTLPKTAGGDRADFWRFRGLRSSKRNLRKSREDLSAQPVQTKFPAYERVVLREAGFLRPVTIFGPIADVAREKLAREEPDIYQIAKSEPRDAGTDHRSSGIIRLHTIKQIIDQDKHALLDVTPNAVDRLNYAQWYPIVVFLNPDSKQGVKTMRMRLCPESRKSARKLYERSHKLRKNNHHLFTTTINLNSMNDGWYGALKEAIQQQQNQLVWVSEGKADGATSDDLDLHDDRLSYLSAPGSEYSMYSTDSRHTSDYEDTDTEGGAYTDQELDETLNDEVGTPPESAITnullSSEPVREDSSGMHHENQTYPPYSPQAQPQAIHRLDSPGLKPASQQKAEASSPVPYLSPETNPASSASAVNPNVSVTNVSLEEPAPAPPASHASQAGGLGAPGAEAAHVVLRGEGPPLPPHADPAQVYRKEPYPEEMMRQNHILKQPALGHPGQRPDKEPNLAYEPQLPYnullEKQASRDLEQPSYRYEASSYPDQFSRNYDHRLRFEDRIPTYEDQWSYYDDKQPYQPRPFENQHPRDLDSRQHnullEEASERGYnullQRFEEPAPLSYDSRTRYEQLPRTSTLRHEEQPAPAYEVHNRYRPEAQPYSSTGPKSSEPKQYFDQYPRSYEQVPPPGFTSKTGHYEPLHGAAVVPPLIPSSQHKPEVLPSATKPQPPPPTLTEEEEDPAMKPQSVLnullRVKMFENKRSASLENKKDVNDTASFKPPEVASKPPSASLAGPKPVPQSQFSEnullDKTLYRLPEPQKPQVKPPnullDIVRSNHYDPEEDEEYYRKQLSYFnullRRSFESKPSAHLPAGHHSEPAKPVHSQSQPNFSSYSSKGKPETDAVDRSFSEKRYDPAQATPPPPPLPSQYSQPAPPLSSSSLHIHSKGAQGEGNSVSLDFQNSYMSKPDPPPSQSKPATFRPPTREDPPQTFYPQKnullFPDKAPVnullGAEQTQKTITPVYNRFTPKPYTSSARPFERKFESPKFNHNLLPSETVHKPELSSKTPTSPKTLMKAHSSTQPPEFDSGVETFSVHTDnullPKYQMNNISTMPKAVPVSPSAVEEDEDEDGHTVVATARGIFNSNGGVLSSIETGVSIIIPQGAIPEGIEQEIYFKVCRDNSILPPLDKEKGETLLSPLVMCGPHGLKFLKPVELRLPHCASMTPDGWSFALKSSDSSSGDPKTWQNKCLPGDPNYLVGANCVSVLIDHF

Target ORF information:

RefSeq Version	XM_021166164.1
Organism	Mus caroli(Ryukyu mouse)
Definition	Mus caroli tight junction protein 1 (Tjp1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021166164.1

ORF Insert Sequence:

ATGTCCACCA GGGCCGCGGC CGCTAAGAGC ACAGCAATGG AGGAAACGGC TATATGGGAA 
CAGCACACAG TGATGCTTCA CAGGGCTCCT GGGTTTGGAT TTGGAATTGC AATCTCTGGT 
GGAAGAGATA ATCCTCATTT TCAGAGTGGG GAAACCTCCA TAGTGATTTC TGATGTGCTA 
AAAGGAGGGC CAGCTGAAGG ACAGCTACAG GAAAATGACC GAGTTGCAAT GGTTAATGGA 
GTTTCAATGG ATAACGTTGA ACATGCTTTT GCTGTTCAGC AGCTAAGGAA AAGTGGGAAA 
AACGCAAAAA TTACTATGCG AAGGAAGAAG AAAGTTCAGA TCCCTGTAAG TCACCCAGAT 
CCTGAGCCGG TGTCTGATAA TGAAGATGAT AGCTACGACG AAGAAGTACA TGATCCAAGA 
GCTGGCCGCA GTGCTTTAGC GAACAGAAGG AGCGAGAAGA GCTGGGCAAG GGATAGGAGT 
GCAAGCAGGG AGAGGAGCCT GTCCCCTCGC TCGGACAGGC GGTCGGTGGC CTCCAGTCAG 
CCCGCAAAAC CCACCAAGGT CGCACTGGTG AAGTCTCGGA AAAATGAAGA ATATGGTCTT 
CGATTGGCCA GCCATATATT TGTAAAGGAA ATTTCACAAG ATAGTTTGGC AGCAAGAGAT 
GGTAACATTC AAGAAGGGGA TGTTGTCTTG AAGATAAATG GTACTGTGAC AGAAAATATG 
TCATTGACAG ATGCAAAAAC ACTGATAGAA AGGTCTAAAG GCAAGTTAAA AATGGTAGTG 
CAAAGAGATG AGCGGGCTAC CTTACTGAAC GTCCCTGACC TTTCGGATAG TATCCATTCT 
GCTAATGCCT CGGAAAGAGA TGACATTTCA GAAATTCAGT CACTAGCATC AGACCATTCA 
GGTCGCTCGC ATGACAGGCC ACCCCGCCGC AGCCAGTCAC GATCTCCTGA CCAACGTTCA 
GAGCCCTCCG ATCATTCCAC ACAGTCTCCA CAGCAGCCCA GCAATGGCAG TCTCCGGAGC 
AGAGAGCAAG AGCGAATGTC TAAACCTGGG GCCATCTCAA CTCCTGTAAA ACATGCAGAT 
GATCATACAC CCAAAGCAGT GGAAGAAGTT ACAGTTGAGA AAAATGAGAA GCAGACACCC 
ACTCTTCCAG AACCGAAGCC TGTATATGCT CAAGTTGGAC AACCAGATGT GGATTTACCC 
GTCAGCCCTT CTGATGGTGT TCTGCCTAAT TCAACTCATG AAGATGGGAT ACTTAGGCCC 
AGCATGAAAC TGGTAAAATT CAGAAAAGGA GATAGTGTGG GTTTGCGACT AGCTGGTGGA 
AATGATGTCG GAATATTTGT AGCTGGCGTT CTAGAAGATA GCCCTGCAGC CAAAGAAGGC 
TTAGAGGAAG GTGATCAAAT TCTCAGGGTG AACAATGTAG ATTTCACAAA TATCATAAGA 
GAAGAGGCTG TCCTTTTCCT CCTTGACCTC CCTAAAGGTG AAGAAGTGAC CATCCTGGCT 
CAGAAGAAGA AGGGTGTTTA TCGCCGCATT GTAGAATCAG ATGTAGGAGA TTCATTCTAT 
ATTAGAACGC ATTTTGAGTA TGAAAAAGAA TCTCCTTACG GACTTAGTTT TAACAAAGGA 
GAGGTGTTCC GGGTCGTGGA TACTTTATAC AATGGAAAGC TGGGCTCTTG GCTTGCCATT 
CGAATTGGCA AAAATCATAA GGAGGTAGAA CGAGGCATCA TCCCTAATAA GAACAGAGCT 
GAGCAGTTAG CCAGTGTACA GTACACACTT CCAAAGACAG CGGGTGGTGA TCGGGCAGAC 
TTCTGGAGGT TTCGAGGTCT TCGCAGCTCC AAGAGAAATC TTCGAAAAAG CAGAGAGGAC 
TTGTCAGCTC AGCCAGTTCA AACTAAGTTC CCAGCTTATG AAAGGGTTGT TCTTCGAGAA 
GCTGGATTCC TAAGACCTGT AACCATCTTT GGACCAATAG CTGATGTTGC CAGAGAAAAG 
TTGGCAAGAG AGGAACCAGA TATCTATCAG ATTGCAAAAA GTGAACCACG AGATGCTGGG 
ACTGACCATC GTAGCTCTGG CATCATTCGC CTTCATACAA TAAAGCAAAT CATAGATCAG 
GATAAACATG CGTTATTAGA TGTAACGCCA AATGCGGTTG ATCGTCTTAA TTATGCGCAG 
TGGTATCCAA TTGTTGTGTT CCTTAACCCT GACTCTAAGC AAGGTGTAAA AACAATGAGG 
ATGAGGTTAT GTCCGGAGTC TCGGAAAAGC GCCAGGAAGC TATATGAACG CTCTCATAAG 
CTTCGCAAAA ACAATCACCA CCTTTTCACA ACTACAATTA ACTTAAACTC AATGAATGAT 
GGTTGGTACG GTGCCCTGAA AGAAGCCATT CAGCAGCAAC AGAACCAGCT GGTGTGGGTC 
TCTGAGGGGA AGGCGGATGG TGCTACAAGT GATGACCTTG ATTTGCATGA TGATCGTCTG 
TCCTACCTGT CAGCCCCAGG TAGTGAGTAC TCAATGTATA GCACGGACAG TAGACACACT 
TCTGACTATG AAGACACAGA TACAGAAGGC GGGGCCTACA CTGATCAAGA ACTAGATGAA 
ACTCTTAATG ATGAGGTGGG GACTCCCCCG GAGTCTGCCA TTACACNGTC CTCTGAGCCT 
GTAAGAGAGG ATTCCTCTGG GATGCATCAT GAAAACCAGA CCTATCCTCC TTACTCACCA 
CAAGCGCAGC CACAAGCTAT TCATAGACTA GACTCCCCTG GACTTAAGCC AGCCTCTCAA 
CAGAAAGCAG AAGCCTCATC TCCAGTCCCT TACCTTTCGC CTGAAACAAA CCCAGCATCG 
TCAGCCTCTG CAGTTAATCC TAATGTCAGT GTAACTAATG TCAGCCTGGA GGAGCCTGCC 
CCAGCCCCTC CCGCCTCGCA CGCATCCCAG GCTGGTGGTT TAGGAGCACC AGGTGCTGAG 
GCAGCTCACG TAGTGCTCAG AGGTGAAGGA CCACCATTGC CGCCGCATGC AGACCCAGCA 
CAGGTGTATA GAAAGGAGCC GTATCCTGAA GAAATGATGA GACAAAACCA TATTTTAAAA 
CAACCAGCTC TTGGTCACCC AGGGCAGAGG CCAGATAAAG AGCCAAATCT AGCCTATGAA 
CCCCAACTTC CATATANAGA AAAACAAGCC AGCAGAGACC TTGAACAGCC GTCATACAGG 
TATGAGGCCT CAAGCTACCC AGACCAGTTT TCTCGGAACT ATGACCATCG CCTACGGTTT 
GAAGATCGAA TCCCTACCTA TGAAGACCAG TGGTCATATT ATGATGACAA ACAGCCCTAC 
CAACCTCGGC CTTTTGAGAA TCAGCATCCC CGAGACCTGG ACTCCAGACA ACATCCNGAG 
GAGGCTTCAG AACGAGGTTA TNTCCAGCGT TTTGAAGAGC CAGCCCCTCT GTCATACGAC 
AGTAGAACAC GCTATGAGCA GCTGCCTCGA ACCTCTACTC TACGACATGA AGAGCAGCCA 
GCCCCTGCAT ATGAGGTGCA CAACAGGTAC AGGCCAGAGG CACAGCCCTA TTCTTCAACA 
GGTCCTAAGT CATCTGAGCC CAAGCAGTAC TTTGACCAGT ACCCGCGAAG TTATGAGCAA 
GTACCACCAC CAGGCTTTAC CTCCAAAACA GGCCATTACG AGCCTCTCCA TGGTGCTGCA 
GTTGTCCCTC CTCTGATACC TTCCTCTCAA CATAAGCCAG AAGTCCTGCC CTCGGCTACC 
AAACCACAGC CTCCACCCCC AACCCTAACT GAGGAGGAGG AGGATCCAGC AATGAAACCA 
CAGTCCGTGC TCANCAGAGT CAAAATGTTT GAAAACAAAA GATCTGCGTC TTTGGAGAAC 
AAGAAAGATG TGAATGACAC TGCCAGCTTT AAGCCTCCAG AAGTAGCATC TAAACCTCCA 
AGTGCTTCTC TTGCTGGCCC TAAACCTGTT CCTCAGAGTC AGTTTAGTGA GCANGACAAA 
ACACTCTACA GGCTCCCAGA GCCTCAGAAA CCTCAAGTGA AGCCACCCGA NGATATTGTT 
CGATCAAATC ATTACGACCC TGAAGAGGAT GAAGAATATT ACAGGAAACA GCTCTCTTAC 
TTTGANCGAA GAAGTTTTGA GAGCAAGCCT TCTGCACATC TTCCTGCTGG CCATCACTCA 
GAGCCTGCTA AGCCAGTCCA TTCTCAGAGC CAGCCCAACT TTTCTAGTTA CTCTTCAAAG 
GGAAAACCCG AAACTGATGC TGTGGATAGA TCATTCAGTG AGAAACGTTA TGATCCAGCC 
CAGGCCACGC CTCCTCCTCC TCCGTTGCCC TCACAGTACA GCCAGCCAGC TCCCCCTCTG 
TCCAGCTCTT CTCTCCACAT ACATTCCAAG GGCGCCCAGG GCGAAGGCAA TTCAGTATCA 
TTGGATTTTC AGAACTCATA TATGTCCAAA CCAGACCCAC CCCCATCTCA GAGTAAACCA 
GCAACTTTCA GACCACCAAC CCGAGAAGAC CCTCCTCAGA CTTTCTATCC CCAGAAANGT 
TTCCCAGACA AAGCTCCAGT TAANGGAGCT GAGCAGACTC AGAAAACCAT CACTCCAGTG 
TACAATCGAT TCACACCAAA GCCGTACACA AGTTCTGCCC GGCCGTTTGA ACGCAAATTT 
GAAAGTCCGA AGTTCAACCA TAATCTTCTG CCAAGTGAAA CTGTACATAA ACCTGAATTG 
TCTTCAAAAA CTCCCACTTC TCCAAAAACT CTTATGAAAG CTCATAGTTC AACACAGCCT 
CCAGAGTTTG ACAGTGGAGT TGAAACTTTC TCTGTTCACA CAGATNAGCC TAAATATCAA 
ATGAATAATA TCAGCACCAT GCCTAAAGCT GTCCCTGTGA GTCCTTCAGC TGTGGAAGAA 
GATGAAGATG AGGATGGTCA TACTGTAGTG GCTACAGCTC GTGGCATTTT TAACAGCAAT 
GGTGGTGTGT TGAGTTCCAT AGAAACCGGT GTTAGTATAA TTATCCCACA AGGAGCCATT 
CCTGAAGGAA TTGAACAAGA AATCTATTTC AAAGTCTGCA GAGACAATAG CATTCTCCCA 
CCTTTAGATA AAGAGAAAGG TGAAACTCTG CTAAGTCCCC TAGTGATGTG TGGGCCCCAT 
GGCCTCAAGT TCCTGAAGCC CGTGGAGCTG CGCTTGCCAC ACTGTGCGTC CATGACTCCT 
GACGGTTGGT CTTTTGCTCT AAAATCATCC GACTCCTCGT CGGGTGACCC TAAAACCTGG 
CAAAACAAGT GTCTTCCTGG AGATCCGAAT TACCTTGTTG GAGCCAACTG TGTTTCTGTC 
CTGATTGACC ACTTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Tjp1 ( XM_021166164.2 ) cDNA ORF clone, Mus caroli(Ryukyu mouse) -> XP_021021823.1 Mus caroli tight junction protein 1 (Tjp1), transcript variant X6, mRNA.

Price & Availability↑

CloneID	OMv03978
Clone ID Related Accession (Same CDS sequence)	XM_021166164.1 , XM_021166164.2
Accession Version	XM_021166164.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5298bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-06-09
Organism	Mus caroli(Ryukyu mouse)
Product	tight junction protein ZO-1 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_034576.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process On Jun 10, 2019 this sequence version replaced XM_021166164.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus caroli Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCACCA GGGCCGCGGC CGCTAAGAGC ACAGCAATGG AGGAAACGGC TATATGGGAA 
CAGCACACAG TGATGCTTCA CAGGGCTCCT GGGTTTGGAT TTGGAATTGC AATCTCTGGT 
GGAAGAGATA ATCCTCATTT TCAGAGTGGG GAAACCTCCA TAGTGATTTC TGATGTGCTA 
AAAGGAGGGC CAGCTGAAGG ACAGCTACAG GAAAATGACC GAGTTGCAAT GGTTAATGGA 
GTTTCAATGG ATAACGTTGA ACATGCTTTT GCTGTTCAGC AGCTAAGGAA AAGTGGGAAA 
AACGCAAAAA TTACTATGCG AAGGAAGAAG AAAGTTCAGA TCCCTGTAAG TCACCCAGAT 
CCTGAGCCGG TGTCTGATAA TGAAGATGAT AGCTACGACG AAGAAGTACA TGATCCAAGA 
GCTGGCCGCA GTGCTTTAGC GAACAGAAGG AGCGAGAAGA GCTGGGCAAG GGATAGGAGT 
GCAAGCAGGG AGAGGAGCCT GTCCCCTCGC TCGGACAGGC GGTCGGTGGC CTCCAGTCAG 
CCCGCAAAAC CCACCAAGGT CGCACTGGTG AAGTCTCGGA AAAATGAAGA ATATGGTCTT 
CGATTGGCCA GCCATATATT TGTAAAGGAA ATTTCACAAG ATAGTTTGGC AGCAAGAGAT 
GGTAACATTC AAGAAGGGGA TGTTGTCTTG AAGATAAATG GTACTGTGAC AGAAAATATG 
TCATTGACAG ATGCAAAAAC ACTGATAGAA AGGTCTAAAG GCAAGTTAAA AATGGTAGTG 
CAAAGAGATG AGCGGGCTAC CTTACTGAAC GTCCCTGACC TTTCGGATAG TATCCATTCT 
GCTAATGCCT CGGAAAGAGA TGACATTTCA GAAATTCAGT CACTAGCATC AGACCATTCA 
GGTCGCTCGC ATGACAGGCC ACCCCGCCGC AGCCAGTCAC GATCTCCTGA CCAACGTTCA 
GAGCCCTCCG ATCATTCCAC ACAGTCTCCA CAGCAGCCCA GCAATGGCAG TCTCCGGAGC 
AGAGAGCAAG AGCGAATGTC TAAACCTGGG GCCATCTCAA CTCCTGTAAA ACATGCAGAT 
GATCATACAC CCAAAGCAGT GGAAGAAGTT ACAGTTGAGA AAAATGAGAA GCAGACACCC 
ACTCTTCCAG AACCGAAGCC TGTATATGCT CAAGTTGGAC AACCAGATGT GGATTTACCC 
GTCAGCCCTT CTGATGGTGT TCTGCCTAAT TCAACTCATG AAGATGGGAT ACTTAGGCCC 
AGCATGAAAC TGGTAAAATT CAGAAAAGGA GATAGTGTGG GTTTGCGACT AGCTGGTGGA 
AATGATGTCG GAATATTTGT AGCTGGCGTT CTAGAAGATA GCCCTGCAGC CAAAGAAGGC 
TTAGAGGAAG GTGATCAAAT TCTCAGGGTG AACAATGTAG ATTTCACAAA TATCATAAGA 
GAAGAGGCTG TCCTTTTCCT CCTTGACCTC CCTAAAGGTG AAGAAGTGAC CATCCTGGCT 
CAGAAGAAGA AGGGTGTTTA TCGCCGCATT GTAGAATCAG ATGTAGGAGA TTCATTCTAT 
ATTAGAACGC ATTTTGAGTA TGAAAAAGAA TCTCCTTACG GACTTAGTTT TAACAAAGGA 
GAGGTGTTCC GGGTCGTGGA TACTTTATAC AATGGAAAGC TGGGCTCTTG GCTTGCCATT 
CGAATTGGCA AAAATCATAA GGAGGTAGAA CGAGGCATCA TCCCTAATAA GAACAGAGCT 
GAGCAGTTAG CCAGTGTACA GTACACACTT CCAAAGACAG CGGGTGGTGA TCGGGCAGAC 
TTCTGGAGGT TTCGAGGTCT TCGCAGCTCC AAGAGAAATC TTCGAAAAAG CAGAGAGGAC 
TTGTCAGCTC AGCCAGTTCA AACTAAGTTC CCAGCTTATG AAAGGGTTGT TCTTCGAGAA 
GCTGGATTCC TAAGACCTGT AACCATCTTT GGACCAATAG CTGATGTTGC CAGAGAAAAG 
TTGGCAAGAG AGGAACCAGA TATCTATCAG ATTGCAAAAA GTGAACCACG AGATGCTGGG 
ACTGACCATC GTAGCTCTGG CATCATTCGC CTTCATACAA TAAAGCAAAT CATAGATCAG 
GATAAACATG CGTTATTAGA TGTAACGCCA AATGCGGTTG ATCGTCTTAA TTATGCGCAG 
TGGTATCCAA TTGTTGTGTT CCTTAACCCT GACTCTAAGC AAGGTGTAAA AACAATGAGG 
ATGAGGTTAT GTCCGGAGTC TCGGAAAAGC GCCAGGAAGC TATATGAACG CTCTCATAAG 
CTTCGCAAAA ACAATCACCA CCTTTTCACA ACTACAATTA ACTTAAACTC AATGAATGAT 
GGTTGGTACG GTGCCCTGAA AGAAGCCATT CAGCAGCAAC AGAACCAGCT GGTGTGGGTC 
TCTGAGGGGA AGGCGGATGG TGCTACAAGT GATGACCTTG ATTTGCATGA TGATCGTCTG 
TCCTACCTGT CAGCCCCAGG TAGTGAGTAC TCAATGTATA GCACGGACAG TAGACACACT 
TCTGACTATG AAGACACAGA TACAGAAGGC GGGGCCTACA CTGATCAAGA ACTAGATGAA 
ACTCTTAATG ATGAGGTGGG GACTCCCCCG GAGTCTGCCA TTACACNGTC CTCTGAGCCT 
GTAAGAGAGG ATTCCTCTGG GATGCATCAT GAAAACCAGA CCTATCCTCC TTACTCACCA 
CAAGCGCAGC CACAAGCTAT TCATAGACTA GACTCCCCTG GACTTAAGCC AGCCTCTCAA 
CAGAAAGCAG AAGCCTCATC TCCAGTCCCT TACCTTTCGC CTGAAACAAA CCCAGCATCG 
TCAGCCTCTG CAGTTAATCC TAATGTCAGT GTAACTAATG TCAGCCTGGA GGAGCCTGCC 
CCAGCCCCTC CCGCCTCGCA CGCATCCCAG GCTGGTGGTT TAGGAGCACC AGGTGCTGAG 
GCAGCTCACG TAGTGCTCAG AGGTGAAGGA CCACCATTGC CGCCGCATGC AGACCCAGCA 
CAGGTGTATA GAAAGGAGCC GTATCCTGAA GAAATGATGA GACAAAACCA TATTTTAAAA 
CAACCAGCTC TTGGTCACCC AGGGCAGAGG CCAGATAAAG AGCCAAATCT AGCCTATGAA 
CCCCAACTTC CATATANAGA AAAACAAGCC AGCAGAGACC TTGAACAGCC GTCATACAGG 
TATGAGGCCT CAAGCTACCC AGACCAGTTT TCTCGGAACT ATGACCATCG CCTACGGTTT 
GAAGATCGAA TCCCTACCTA TGAAGACCAG TGGTCATATT ATGATGACAA ACAGCCCTAC 
CAACCTCGGC CTTTTGAGAA TCAGCATCCC CGAGACCTGG ACTCCAGACA ACATCCNGAG 
GAGGCTTCAG AACGAGGTTA TNTCCAGCGT TTTGAAGAGC CAGCCCCTCT GTCATACGAC 
AGTAGAACAC GCTATGAGCA GCTGCCTCGA ACCTCTACTC TACGACATGA AGAGCAGCCA 
GCCCCTGCAT ATGAGGTGCA CAACAGGTAC AGGCCAGAGG CACAGCCCTA TTCTTCAACA 
GGTCCTAAGT CATCTGAGCC CAAGCAGTAC TTTGACCAGT ACCCGCGAAG TTATGAGCAA 
GTACCACCAC CAGGCTTTAC CTCCAAAACA GGCCATTACG AGCCTCTCCA TGGTGCTGCA 
GTTGTCCCTC CTCTGATACC TTCCTCTCAA CATAAGCCAG AAGTCCTGCC CTCGGCTACC 
AAACCACAGC CTCCACCCCC AACCCTAACT GAGGAGGAGG AGGATCCAGC AATGAAACCA 
CAGTCCGTGC TCANCAGAGT CAAAATGTTT GAAAACAAAA GATCTGCGTC TTTGGAGAAC 
AAGAAAGATG TGAATGACAC TGCCAGCTTT AAGCCTCCAG AAGTAGCATC TAAACCTCCA 
AGTGCTTCTC TTGCTGGCCC TAAACCTGTT CCTCAGAGTC AGTTTAGTGA GCANGACAAA 
ACACTCTACA GGCTCCCAGA GCCTCAGAAA CCTCAAGTGA AGCCACCCGA NGATATTGTT 
CGATCAAATC ATTACGACCC TGAAGAGGAT GAAGAATATT ACAGGAAACA GCTCTCTTAC 
TTTGANCGAA GAAGTTTTGA GAGCAAGCCT TCTGCACATC TTCCTGCTGG CCATCACTCA 
GAGCCTGCTA AGCCAGTCCA TTCTCAGAGC CAGCCCAACT TTTCTAGTTA CTCTTCAAAG 
GGAAAACCCG AAACTGATGC TGTGGATAGA TCATTCAGTG AGAAACGTTA TGATCCAGCC 
CAGGCCACGC CTCCTCCTCC TCCGTTGCCC TCACAGTACA GCCAGCCAGC TCCCCCTCTG 
TCCAGCTCTT CTCTCCACAT ACATTCCAAG GGCGCCCAGG GCGAAGGCAA TTCAGTATCA 
TTGGATTTTC AGAACTCATA TATGTCCAAA CCAGACCCAC CCCCATCTCA GAGTAAACCA 
GCAACTTTCA GACCACCAAC CCGAGAAGAC CCTCCTCAGA CTTTCTATCC CCAGAAANGT 
TTCCCAGACA AAGCTCCAGT TAANGGAGCT GAGCAGACTC AGAAAACCAT CACTCCAGTG 
TACAATCGAT TCACACCAAA GCCGTACACA AGTTCTGCCC GGCCGTTTGA ACGCAAATTT 
GAAAGTCCGA AGTTCAACCA TAATCTTCTG CCAAGTGAAA CTGTACATAA ACCTGAATTG 
TCTTCAAAAA CTCCCACTTC TCCAAAAACT CTTATGAAAG CTCATAGTTC AACACAGCCT 
CCAGAGTTTG ACAGTGGAGT TGAAACTTTC TCTGTTCACA CAGATNAGCC TAAATATCAA 
ATGAATAATA TCAGCACCAT GCCTAAAGCT GTCCCTGTGA GTCCTTCAGC TGTGGAAGAA 
GATGAAGATG AGGATGGTCA TACTGTAGTG GCTACAGCTC GTGGCATTTT TAACAGCAAT 
GGTGGTGTGT TGAGTTCCAT AGAAACCGGT GTTAGTATAA TTATCCCACA AGGAGCCATT 
CCTGAAGGAA TTGAACAAGA AATCTATTTC AAAGTCTGCA GAGACAATAG CATTCTCCCA 
CCTTTAGATA AAGAGAAAGG TGAAACTCTG CTAAGTCCCC TAGTGATGTG TGGGCCCCAT 
GGCCTCAAGT TCCTGAAGCC CGTGGAGCTG CGCTTGCCAC ACTGTGCGTC CATGACTCCT 
GACGGTTGGT CTTTTGCTCT AAAATCATCC GACTCCTCGT CGGGTGACCC TAAAACCTGG 
CAAAACAAGT GTCTTCCTGG AGATCCGAAT TACCTTGTTG GAGCCAACTG TGTTTCTGTC 
CTGATTGACC ACTTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021021823.1
CDS	65..5362
Translation	MSTRAAAAKSTAMEETAIWEQHTVMLHRAPGFGFGIAISGGRDNPHFQSGETSIVISDVLKGGPAEGQLQENDRVAMVNGVSMDNVEHAFAVQQLRKSGKNAKITMRRKKKVQIPVSHPDPEPVSDNEDDSYDEEVHDPRAGRSALANRRSEKSWARDRSASRERSLSPRSDRRSVASSQPAKPTKVALVKSRKNEEYGLRLASHIFVKEISQDSLAARDGNIQEGDVVLKINGTVTENMSLTDAKTLIERSKGKLKMVVQRDERATLLNVPDLSDSIHSANASERDDISEIQSLASDHSGRSHDRPPRRSQSRSPDQRSEPSDHSTQSPQQPSNGSLRSREQERMSKPGAISTPVKHADDHTPKAVEEVTVEKNEKQTPTLPEPKPVYAQVGQPDVDLPVSPSDGVLPNSTHEDGILRPSMKLVKFRKGDSVGLRLAGGNDVGIFVAGVLEDSPAAKEGLEEGDQILRVNNVDFTNIIREEAVLFLLDLPKGEEVTILAQKKKGVYRRIVESDVGDSFYIRTHFEYEKESPYGLSFNKGEVFRVVDTLYNGKLGSWLAIRIGKNHKEVERGIIPNKNRAEQLASVQYTLPKTAGGDRADFWRFRGLRSSKRNLRKSREDLSAQPVQTKFPAYERVVLREAGFLRPVTIFGPIADVAREKLAREEPDIYQIAKSEPRDAGTDHRSSGIIRLHTIKQIIDQDKHALLDVTPNAVDRLNYAQWYPIVVFLNPDSKQGVKTMRMRLCPESRKSARKLYERSHKLRKNNHHLFTTTINLNSMNDGWYGALKEAIQQQQNQLVWVSEGKADGATSDDLDLHDDRLSYLSAPGSEYSMYSTDSRHTSDYEDTDTEGGAYTDQELDETLNDEVGTPPESAITnullSSEPVREDSSGMHHENQTYPPYSPQAQPQAIHRLDSPGLKPASQQKAEASSPVPYLSPETNPASSASAVNPNVSVTNVSLEEPAPAPPASHASQAGGLGAPGAEAAHVVLRGEGPPLPPHADPAQVYRKEPYPEEMMRQNHILKQPALGHPGQRPDKEPNLAYEPQLPYnullEKQASRDLEQPSYRYEASSYPDQFSRNYDHRLRFEDRIPTYEDQWSYYDDKQPYQPRPFENQHPRDLDSRQHnullEEASERGYnullQRFEEPAPLSYDSRTRYEQLPRTSTLRHEEQPAPAYEVHNRYRPEAQPYSSTGPKSSEPKQYFDQYPRSYEQVPPPGFTSKTGHYEPLHGAAVVPPLIPSSQHKPEVLPSATKPQPPPPTLTEEEEDPAMKPQSVLnullRVKMFENKRSASLENKKDVNDTASFKPPEVASKPPSASLAGPKPVPQSQFSEnullDKTLYRLPEPQKPQVKPPnullDIVRSNHYDPEEDEEYYRKQLSYFnullRRSFESKPSAHLPAGHHSEPAKPVHSQSQPNFSSYSSKGKPETDAVDRSFSEKRYDPAQATPPPPPLPSQYSQPAPPLSSSSLHIHSKGAQGEGNSVSLDFQNSYMSKPDPPPSQSKPATFRPPTREDPPQTFYPQKnullFPDKAPVnullGAEQTQKTITPVYNRFTPKPYTSSARPFERKFESPKFNHNLLPSETVHKPELSSKTPTSPKTLMKAHSSTQPPEFDSGVETFSVHTDnullPKYQMNNISTMPKAVPVSPSAVEEDEDEDGHTVVATARGIFNSNGGVLSSIETGVSIIIPQGAIPEGIEQEIYFKVCRDNSILPPLDKEKGETLLSPLVMCGPHGLKFLKPVELRLPHCASMTPDGWSFALKSSDSSSGDPKTWQNKCLPGDPNYLVGANCVSVLIDHF

Target ORF information:

RefSeq Version	XM_021166164.2
Organism	Mus caroli(Ryukyu mouse)
Definition	Mus caroli tight junction protein 1 (Tjp1), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021166164.2

ORF Insert Sequence:

ATGTCCACCA GGGCCGCGGC CGCTAAGAGC ACAGCAATGG AGGAAACGGC TATATGGGAA 
CAGCACACAG TGATGCTTCA CAGGGCTCCT GGGTTTGGAT TTGGAATTGC AATCTCTGGT 
GGAAGAGATA ATCCTCATTT TCAGAGTGGG GAAACCTCCA TAGTGATTTC TGATGTGCTA 
AAAGGAGGGC CAGCTGAAGG ACAGCTACAG GAAAATGACC GAGTTGCAAT GGTTAATGGA 
GTTTCAATGG ATAACGTTGA ACATGCTTTT GCTGTTCAGC AGCTAAGGAA AAGTGGGAAA 
AACGCAAAAA TTACTATGCG AAGGAAGAAG AAAGTTCAGA TCCCTGTAAG TCACCCAGAT 
CCTGAGCCGG TGTCTGATAA TGAAGATGAT AGCTACGACG AAGAAGTACA TGATCCAAGA 
GCTGGCCGCA GTGCTTTAGC GAACAGAAGG AGCGAGAAGA GCTGGGCAAG GGATAGGAGT 
GCAAGCAGGG AGAGGAGCCT GTCCCCTCGC TCGGACAGGC GGTCGGTGGC CTCCAGTCAG 
CCCGCAAAAC CCACCAAGGT CGCACTGGTG AAGTCTCGGA AAAATGAAGA ATATGGTCTT 
CGATTGGCCA GCCATATATT TGTAAAGGAA ATTTCACAAG ATAGTTTGGC AGCAAGAGAT 
GGTAACATTC AAGAAGGGGA TGTTGTCTTG AAGATAAATG GTACTGTGAC AGAAAATATG 
TCATTGACAG ATGCAAAAAC ACTGATAGAA AGGTCTAAAG GCAAGTTAAA AATGGTAGTG 
CAAAGAGATG AGCGGGCTAC CTTACTGAAC GTCCCTGACC TTTCGGATAG TATCCATTCT 
GCTAATGCCT CGGAAAGAGA TGACATTTCA GAAATTCAGT CACTAGCATC AGACCATTCA 
GGTCGCTCGC ATGACAGGCC ACCCCGCCGC AGCCAGTCAC GATCTCCTGA CCAACGTTCA 
GAGCCCTCCG ATCATTCCAC ACAGTCTCCA CAGCAGCCCA GCAATGGCAG TCTCCGGAGC 
AGAGAGCAAG AGCGAATGTC TAAACCTGGG GCCATCTCAA CTCCTGTAAA ACATGCAGAT 
GATCATACAC CCAAAGCAGT GGAAGAAGTT ACAGTTGAGA AAAATGAGAA GCAGACACCC 
ACTCTTCCAG AACCGAAGCC TGTATATGCT CAAGTTGGAC AACCAGATGT GGATTTACCC 
GTCAGCCCTT CTGATGGTGT TCTGCCTAAT TCAACTCATG AAGATGGGAT ACTTAGGCCC 
AGCATGAAAC TGGTAAAATT CAGAAAAGGA GATAGTGTGG GTTTGCGACT AGCTGGTGGA 
AATGATGTCG GAATATTTGT AGCTGGCGTT CTAGAAGATA GCCCTGCAGC CAAAGAAGGC 
TTAGAGGAAG GTGATCAAAT TCTCAGGGTG AACAATGTAG ATTTCACAAA TATCATAAGA 
GAAGAGGCTG TCCTTTTCCT CCTTGACCTC CCTAAAGGTG AAGAAGTGAC CATCCTGGCT 
CAGAAGAAGA AGGGTGTTTA TCGCCGCATT GTAGAATCAG ATGTAGGAGA TTCATTCTAT 
ATTAGAACGC ATTTTGAGTA TGAAAAAGAA TCTCCTTACG GACTTAGTTT TAACAAAGGA 
GAGGTGTTCC GGGTCGTGGA TACTTTATAC AATGGAAAGC TGGGCTCTTG GCTTGCCATT 
CGAATTGGCA AAAATCATAA GGAGGTAGAA CGAGGCATCA TCCCTAATAA GAACAGAGCT 
GAGCAGTTAG CCAGTGTACA GTACACACTT CCAAAGACAG CGGGTGGTGA TCGGGCAGAC 
TTCTGGAGGT TTCGAGGTCT TCGCAGCTCC AAGAGAAATC TTCGAAAAAG CAGAGAGGAC 
TTGTCAGCTC AGCCAGTTCA AACTAAGTTC CCAGCTTATG AAAGGGTTGT TCTTCGAGAA 
GCTGGATTCC TAAGACCTGT AACCATCTTT GGACCAATAG CTGATGTTGC CAGAGAAAAG 
TTGGCAAGAG AGGAACCAGA TATCTATCAG ATTGCAAAAA GTGAACCACG AGATGCTGGG 
ACTGACCATC GTAGCTCTGG CATCATTCGC CTTCATACAA TAAAGCAAAT CATAGATCAG 
GATAAACATG CGTTATTAGA TGTAACGCCA AATGCGGTTG ATCGTCTTAA TTATGCGCAG 
TGGTATCCAA TTGTTGTGTT CCTTAACCCT GACTCTAAGC AAGGTGTAAA AACAATGAGG 
ATGAGGTTAT GTCCGGAGTC TCGGAAAAGC GCCAGGAAGC TATATGAACG CTCTCATAAG 
CTTCGCAAAA ACAATCACCA CCTTTTCACA ACTACAATTA ACTTAAACTC AATGAATGAT 
GGTTGGTACG GTGCCCTGAA AGAAGCCATT CAGCAGCAAC AGAACCAGCT GGTGTGGGTC 
TCTGAGGGGA AGGCGGATGG TGCTACAAGT GATGACCTTG ATTTGCATGA TGATCGTCTG 
TCCTACCTGT CAGCCCCAGG TAGTGAGTAC TCAATGTATA GCACGGACAG TAGACACACT 
TCTGACTATG AAGACACAGA TACAGAAGGC GGGGCCTACA CTGATCAAGA ACTAGATGAA 
ACTCTTAATG ATGAGGTGGG GACTCCCCCG GAGTCTGCCA TTACACNGTC CTCTGAGCCT 
GTAAGAGAGG ATTCCTCTGG GATGCATCAT GAAAACCAGA CCTATCCTCC TTACTCACCA 
CAAGCGCAGC CACAAGCTAT TCATAGACTA GACTCCCCTG GACTTAAGCC AGCCTCTCAA 
CAGAAAGCAG AAGCCTCATC TCCAGTCCCT TACCTTTCGC CTGAAACAAA CCCAGCATCG 
TCAGCCTCTG CAGTTAATCC TAATGTCAGT GTAACTAATG TCAGCCTGGA GGAGCCTGCC 
CCAGCCCCTC CCGCCTCGCA CGCATCCCAG GCTGGTGGTT TAGGAGCACC AGGTGCTGAG 
GCAGCTCACG TAGTGCTCAG AGGTGAAGGA CCACCATTGC CGCCGCATGC AGACCCAGCA 
CAGGTGTATA GAAAGGAGCC GTATCCTGAA GAAATGATGA GACAAAACCA TATTTTAAAA 
CAACCAGCTC TTGGTCACCC AGGGCAGAGG CCAGATAAAG AGCCAAATCT AGCCTATGAA 
CCCCAACTTC CATATANAGA AAAACAAGCC AGCAGAGACC TTGAACAGCC GTCATACAGG 
TATGAGGCCT CAAGCTACCC AGACCAGTTT TCTCGGAACT ATGACCATCG CCTACGGTTT 
GAAGATCGAA TCCCTACCTA TGAAGACCAG TGGTCATATT ATGATGACAA ACAGCCCTAC 
CAACCTCGGC CTTTTGAGAA TCAGCATCCC CGAGACCTGG ACTCCAGACA ACATCCNGAG 
GAGGCTTCAG AACGAGGTTA TNTCCAGCGT TTTGAAGAGC CAGCCCCTCT GTCATACGAC 
AGTAGAACAC GCTATGAGCA GCTGCCTCGA ACCTCTACTC TACGACATGA AGAGCAGCCA 
GCCCCTGCAT ATGAGGTGCA CAACAGGTAC AGGCCAGAGG CACAGCCCTA TTCTTCAACA 
GGTCCTAAGT CATCTGAGCC CAAGCAGTAC TTTGACCAGT ACCCGCGAAG TTATGAGCAA 
GTACCACCAC CAGGCTTTAC CTCCAAAACA GGCCATTACG AGCCTCTCCA TGGTGCTGCA 
GTTGTCCCTC CTCTGATACC TTCCTCTCAA CATAAGCCAG AAGTCCTGCC CTCGGCTACC 
AAACCACAGC CTCCACCCCC AACCCTAACT GAGGAGGAGG AGGATCCAGC AATGAAACCA 
CAGTCCGTGC TCANCAGAGT CAAAATGTTT GAAAACAAAA GATCTGCGTC TTTGGAGAAC 
AAGAAAGATG TGAATGACAC TGCCAGCTTT AAGCCTCCAG AAGTAGCATC TAAACCTCCA 
AGTGCTTCTC TTGCTGGCCC TAAACCTGTT CCTCAGAGTC AGTTTAGTGA GCANGACAAA 
ACACTCTACA GGCTCCCAGA GCCTCAGAAA CCTCAAGTGA AGCCACCCGA NGATATTGTT 
CGATCAAATC ATTACGACCC TGAAGAGGAT GAAGAATATT ACAGGAAACA GCTCTCTTAC 
TTTGANCGAA GAAGTTTTGA GAGCAAGCCT TCTGCACATC TTCCTGCTGG CCATCACTCA 
GAGCCTGCTA AGCCAGTCCA TTCTCAGAGC CAGCCCAACT TTTCTAGTTA CTCTTCAAAG 
GGAAAACCCG AAACTGATGC TGTGGATAGA TCATTCAGTG AGAAACGTTA TGATCCAGCC 
CAGGCCACGC CTCCTCCTCC TCCGTTGCCC TCACAGTACA GCCAGCCAGC TCCCCCTCTG 
TCCAGCTCTT CTCTCCACAT ACATTCCAAG GGCGCCCAGG GCGAAGGCAA TTCAGTATCA 
TTGGATTTTC AGAACTCATA TATGTCCAAA CCAGACCCAC CCCCATCTCA GAGTAAACCA 
GCAACTTTCA GACCACCAAC CCGAGAAGAC CCTCCTCAGA CTTTCTATCC CCAGAAANGT 
TTCCCAGACA AAGCTCCAGT TAANGGAGCT GAGCAGACTC AGAAAACCAT CACTCCAGTG 
TACAATCGAT TCACACCAAA GCCGTACACA AGTTCTGCCC GGCCGTTTGA ACGCAAATTT 
GAAAGTCCGA AGTTCAACCA TAATCTTCTG CCAAGTGAAA CTGTACATAA ACCTGAATTG 
TCTTCAAAAA CTCCCACTTC TCCAAAAACT CTTATGAAAG CTCATAGTTC AACACAGCCT 
CCAGAGTTTG ACAGTGGAGT TGAAACTTTC TCTGTTCACA CAGATNAGCC TAAATATCAA 
ATGAATAATA TCAGCACCAT GCCTAAAGCT GTCCCTGTGA GTCCTTCAGC TGTGGAAGAA 
GATGAAGATG AGGATGGTCA TACTGTAGTG GCTACAGCTC GTGGCATTTT TAACAGCAAT 
GGTGGTGTGT TGAGTTCCAT AGAAACCGGT GTTAGTATAA TTATCCCACA AGGAGCCATT 
CCTGAAGGAA TTGAACAAGA AATCTATTTC AAAGTCTGCA GAGACAATAG CATTCTCCCA 
CCTTTAGATA AAGAGAAAGG TGAAACTCTG CTAAGTCCCC TAGTGATGTG TGGGCCCCAT 
GGCCTCAAGT TCCTGAAGCC CGTGGAGCTG CGCTTGCCAC ACTGTGCGTC CATGACTCCT 
GACGGTTGGT CTTTTGCTCT AAAATCATCC GACTCCTCGT CGGGTGACCC TAAAACCTGG 
CAAAACAAGT GTCTTCCTGG AGATCCGAAT TACCTTGTTG GAGCCAACTG TGTTTCTGTC 
CTGATTGACC ACTTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Tjp1 ( XM_021166165.1 ) cDNA ORF clone, Mus caroli(Ryukyu mouse) -> XP_021021824.1 Mus caroli tight junction protein 1 (Tjp1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OMv03979
Clone ID Related Accession (Same CDS sequence)	XM_021166165.1 , XM_021166165.2
Accession Version	XM_021166165.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5238bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-05-20
Organism	Mus caroli(Ryukyu mouse)
Product	tight junction protein ZO-1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_034576.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus caroli Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCACCA GGGCCGCGGC CGCTAAGAGC ACAGCAATGG AGGAAACGGC TATATGGGAA 
CAGCACACAG TGATGCTTCA CAGGGCTCCT GGGTTTGGAT TTGGAATTGC AATCTCTGGT 
GGAAGAGATA ATCCTCATTT TCAGAGTGGG GAAACCTCCA TAGTGATTTC TGATGTGCTA 
AAAGGAGGGC CAGCTGAAGG ACAGCTACAG GAAAATGACC GAGTTGCAAT GGTTAATGGA 
GTTTCAATGG ATAACGTTGA ACATGCTTTT GCTGTTCAGC AGCTAAGGAA AAGTGGGAAA 
AACGCAAAAA TTACTATGCG AAGGAAGAAG AAAGTTCAGA TCCCTGTAAG TCACCCAGAT 
CCTGAGCCGG TGTCTGATAA TGAAGATGAT AGCTACGACG AAGAAGTACA TGATCCAAGA 
GCTGGCCGCA GTGCTTTAGC GAACAGAAGG AGCGAGAAGA GCTGGGCAAG GGATAGGAGT 
GCAAGCAGGG AGAGGAGCCT GTCCCCTCGC TCGGACAGGC GGTCGGTGGC CTCCAGTCAG 
CCCGCAAAAC CCACCAAGGT CGCACTGGTG AAGTCTCGGA AAAATGAAGA ATATGGTCTT 
CGATTGGCCA GCCATATATT TGTAAAGGAA ATTTCACAAG ATAGTTTGGC AGCAAGAGAT 
GGTAACATTC AAGAAGGGGA TGTTGTCTTG AAGATAAATG GTACTGTGAC AGAAAATATG 
TCATTGACAG ATGCAAAAAC ACTGATAGAA AGGTCTAAAG GCAAGTTAAA AATGGTAGTG 
CAAAGAGATG AGCGGGCTAC CTTACTGAAC GTCCCTGACC TTTCGGATAG TATCCATTCT 
GCTAATGCCT CGGAAAGAGA TGACATTTCA GAAATTCAGT CACTAGCATC AGACCATTCA 
GGTCGCTCGC ATGACAGGCC ACCCCGCCGC AGCCAGTCAC GATCTCCTGA CCAACGTTCA 
GAGCCCTCCG ATCATTCCAC ACAGTCTCCA CAGCAGCCCA GCAATGGCAG TCTCCGGAGC 
AGAGAGCAAG AGCGAATGTC TAAACCTGGG GCCATCTCAA CTCCTGTAAA ACATGCAGAT 
GATCATACAC CCAAAGCAGT GGAAGAAGTT ACAGTTGAGA AAAATGAGAA GCAGACACCC 
ACTCTTCCAG AACCGAAGCC TGTATATGCT CAAGTTGGAC AACCAGATGT GGATTTACCC 
GTCAGCCCTT CTGATGGTGT TCTGCCTAAT TCAACTCATG AAGATGGGAT ACTTAGGCCC 
AGCATGAAAC TGGTAAAATT CAGAAAAGGA GATAGTGTGG GTTTGCGACT AGCTGGTGGA 
AATGATGTCG GAATATTTGT AGCTGGCGTT CTAGAAGATA GCCCTGCAGC CAAAGAAGGC 
TTAGAGGAAG GTGATCAAAT TCTCAGGGTG AACAATGTAG ATTTCACAAA TATCATAAGA 
GAAGAGGCTG TCCTTTTCCT CCTTGACCTC CCTAAAGGTG AAGAAGTGAC CATCCTGGCT 
CAGAAGAAGA AGGGTGTTTA TCGCCGCATT GTAGAATCAG ATGTAGGAGA TTCATTCTAT 
ATTAGAACGC ATTTTGAGTA TGAAAAAGAA TCTCCTTACG GACTTAGTTT TAACAAAGGA 
GAGGTGTTCC GGGTCGTGGA TACTTTATAC AATGGAAAGC TGGGCTCTTG GCTTGCCATT 
CGAATTGGCA AAAATCATAA GGAGGTAGAA CGAGGCATCA TCCCTAATAA GAACAGAGCT 
GAGCAGTTAG CCAGTGTACA GTACACACTT CCAAAGACAG CGGGTGGTGA TCGGGCAGAC 
TTCTGGAGGT TTCGAGGTCT TCGCAGCTCC AAGAGAAATC TTCGAAAAAG CAGAGAGGAC 
TTGTCAGCTC AGCCAGTTCA AACTAAGTTC CCAGCTTATG AAAGGGTTGT TCTTCGAGAA 
GCTGGATTCC TAAGACCTGT AACCATCTTT GGACCAATAG CTGATGTTGC CAGAGAAAAG 
TTGGCAAGAG AGGAACCAGA TATCTATCAG ATTGCAAAAA GTGAACCACG AGATGCTGGG 
ACTGACCATC GTAGCTCTGG CATCATTCGC CTTCATACAA TAAAGCAAAT CATAGATCAG 
GATAAACATG CGTTATTAGA TGTAACGCCA AATGCGGTTG ATCGTCTTAA TTATGCGCAG 
TGGTATCCAA TTGTTGTGTT CCTTAACCCT GACTCTAAGC AAGGTGTAAA AACAATGAGG 
ATGAGGTTAT GTCCGGAGTC TCGGAAAAGC GCCAGGAAGC TATATGAACG CTCTCATAAG 
CTTCGCAAAA ACAATCACCA CCTTTTCACA ACTACAATTA ACTTAAACTC AATGAATGAT 
GGTTGGTACG GTGCCCTGAA AGAAGCCATT CAGCAGCAAC AGAACCAGCT GGTGTGGGTC 
TCTGAGGGGA AGGCGGATGG TGCTACAAGT GATGACCTTG ATTTGCATGA TGATCGTCTG 
TCCTACCTGT CAGCCCCAGG TAGTGAGTAC TCAATGTATA GCACGGACAG TAGACACACT 
TCTGACTATG AAGACACAGA TACAGAAGGC GGGGCCTACA CTGATCAAGA ACTAGATGAA 
ACTCTTAATG ATGAGGTGGG GACTCCCCCG GAGTCTGCCA TTACACNGTC CTCTGAGCCT 
GTAAGAGAGG ATTCCTCTGG GATGCATCAT GAAAACCAGA CCTATCCTCC TTACTCACCA 
CAAGCGCAGC CACAAGCTAT TCATAGACTA GACTCCCCTG GACTTAAGCC AGCCTCTCAA 
CAGAAAGCAG AAGCCTCATC TCCAGTCCCT TACCTTTCGC CTGAAACAAA CCCAGCATCG 
TCAGCCTCTG CAGTTAATCC TAATGTCAGT GTAACTAATG TCAGCCTGGA GGAGCCTGCC 
CCAGCCCCTC CCGCCTCGCA CGCATCCCAG GCTGGTGGTT TAGGAGCACC AGGTGCTGAG 
GCAGCTCACG TAGTGCTCAG AGGTGAAGGA CCACCATTGC CGCCGCATGC AGACCCAGCA 
CAGGTGTATA GAAAGGAGCC GTATCCTGAA GAAATGATGA GACAAAACCA TATTTTAAAA 
CAACCAGCTC TTGGTCACCC AGGGCAGAGG CCAGATAAAG AGCCAAATCT AGCCTATGAA 
CCCCAACTTC CATATANAGA AAAACAAGCC AGCAGAGACC TTGAACAGCC GTCATACAGG 
TATGAGGCCT CAAGCTACCC AGACCAGTTT TCTCGGAACT ATGACCATCG CCTACGGTTT 
GAAGATCGAA TCCCTACCTA TGAAGACCAG TGGTCATATT ATGATGACAA ACAGCCCTAC 
CAACCTCGGC CTTTTGAGAA TCAGCATCCC CGAGACCTGG ACTCCAGACA ACATCCNGAG 
GAGGCTTCAG AACGAGGTTA TNTCCAGCGT TTTGAAGAGC CAGCCCCTCT GTCATACGAC 
AGTAGAACAC GCTATGAGCA GCTGCCTCGA ACCTCTACTC TACGACATGA AGAGCAGCCA 
GCCCCTGCAT ATGAGGTGCA CAACAGGTAC AGGCCAGAGG CACAGCCCTA TTCTTCAACA 
GGTCCTAAGT CATCTGAGCC CAAGCAGTAC TTTGACCAGT ACCCGCGAAG TTATGAGCAA 
GTACCACCAC CAGGCTTTAC CTCCAAAACA GGCCATTACG AGCCTCTCCA TGGTGCTGCA 
GTTGTCCCTC CTCTGATACC TTCCTCTCAA CATAAGCCAG AAGTCCTGCC CTCGGCTACC 
AAACCACAGC CTCCACCCCC AACCCTAACT GAGGAGGAGG AGGATCCAGC AATGAAACCA 
CAGTCCGTGC TCANCAGAGT CAAAATGTTT GAAAACAAAA GATCTGCGTC TTTGGAGAAC 
AAGAAAGATG TGAATGACAC TGCCAGCTTT AAGCCTCCAG AAGTAGCATC TAAACCTCCA 
AGTGCTTCTC TTGCTGGCCC TAAACCTGTT CCTCAGAGTC AGTTTAGTGA GCANGACAAA 
ACACTCTACA GGCTCCCAGA GCCTCAGAAA CCTCAAGTGA AGCCACCCGA NGATATTGTT 
CGATCAAATC ATTACGACCC TGAAGAGGAT GAAGAATATT ACAGGAAACA GCTCTCTTAC 
TTTGANCGAA GAAGTTTTGA GAGCAAGCCT TCTGCACATC TTCCTGCTGG CCATCACTCA 
GAGCCTGCTA AGCCAGTCCA TTCTCAGAGC CAGCCCAACT TTTCTAGTTA CTCTTCAAAG 
GGAAAACCCG AAACTGATGC TGTGGATAGA TCATTCAGTG AGAAACGTTA TGATCCAGCC 
CAGGCCACGC CTCCTCCTCC TCCGTTGCCC TCACAGTACA GCCAGCCAGC TCCCCCTCTG 
TCCAGCTCTT CTCTCCACAT ACATTCCAAG GGCGCCCAGG GCGAAGGCAA TTCAGTATCA 
TTGGATTTTC AGAACTCATA TATGTCCAAA CCAGACCCAC CCCCATCTCA GAGTAAACCA 
GCAACTTTCA GACCACCAAC CCGAGAAGAC CCTCCTCAGA CTTTCTATCC CCAGAAANGT 
TTCCCAGACA AAGCTCCAGT TAANGGAGCT GAGCAGACTC AGAAAACCAT CACTCCAGTG 
TACAATCGAT TCACACCAAA GCCGTACACA AGTTCTGCCC GGCCGTTTGA ACGCAAATTT 
GAAAGTCCGA AGTTCAACCA TAATCTTCTG CCAAGTGAAA CTGTACATAA ACCTGAATTG 
TCTTCAAAAA CTCCCACTTC TCCAAAAACT CTTATGAAAG CTCATAGTTC AACACAGCCT 
CCAGAGTTTG ACAGTGGAGT TGAAACTTTC TCTGTTCACA CAGATNAGCC TAAATATCAA 
ATGAATAATA TCAGCACCAT GCCTAAAGCT GTCCCTGTGA GTCCTTCAGC TGTGGAAGAA 
GATGAAGATG AGGATGGTCA TACTGTAGTG GCTACAGCTC GTGGCATTTT TAACAGCAAT 
GGTGGTGTGT TGAGTTCCAT AGAAACCGGT GTTAGTATAA TTATCCCACA AGGAGCCATT 
CCTGAAGGAA TTGAACAAGA AATCTATTTC AAAGTCTGCA GAGACAATAG CATTCTCCCA 
CCTTTAGATA AAGAGAAAGG TGAAACTCTG CTAAGTCCCC TAGTGATGTG TGGGCCCCAT 
GGCCTCAAGT TCCTGAAGCC CGTGGAGCTG CGCTTGCCAC ACTGTGACCC TAAAACCTGG 
CAAAACAAGT GTCTTCCTGG AGATCCGAAT TACCTTGTTG GAGCCAACTG TGTTTCTGTC 
CTGATTGACC ACTTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021021824.1
CDS	38..5275
Translation	MSTRAAAAKSTAMEETAIWEQHTVMLHRAPGFGFGIAISGGRDNPHFQSGETSIVISDVLKGGPAEGQLQENDRVAMVNGVSMDNVEHAFAVQQLRKSGKNAKITMRRKKKVQIPVSHPDPEPVSDNEDDSYDEEVHDPRAGRSALANRRSEKSWARDRSASRERSLSPRSDRRSVASSQPAKPTKVALVKSRKNEEYGLRLASHIFVKEISQDSLAARDGNIQEGDVVLKINGTVTENMSLTDAKTLIERSKGKLKMVVQRDERATLLNVPDLSDSIHSANASERDDISEIQSLASDHSGRSHDRPPRRSQSRSPDQRSEPSDHSTQSPQQPSNGSLRSREQERMSKPGAISTPVKHADDHTPKAVEEVTVEKNEKQTPTLPEPKPVYAQVGQPDVDLPVSPSDGVLPNSTHEDGILRPSMKLVKFRKGDSVGLRLAGGNDVGIFVAGVLEDSPAAKEGLEEGDQILRVNNVDFTNIIREEAVLFLLDLPKGEEVTILAQKKKGVYRRIVESDVGDSFYIRTHFEYEKESPYGLSFNKGEVFRVVDTLYNGKLGSWLAIRIGKNHKEVERGIIPNKNRAEQLASVQYTLPKTAGGDRADFWRFRGLRSSKRNLRKSREDLSAQPVQTKFPAYERVVLREAGFLRPVTIFGPIADVAREKLAREEPDIYQIAKSEPRDAGTDHRSSGIIRLHTIKQIIDQDKHALLDVTPNAVDRLNYAQWYPIVVFLNPDSKQGVKTMRMRLCPESRKSARKLYERSHKLRKNNHHLFTTTINLNSMNDGWYGALKEAIQQQQNQLVWVSEGKADGATSDDLDLHDDRLSYLSAPGSEYSMYSTDSRHTSDYEDTDTEGGAYTDQELDETLNDEVGTPPESAITnullSSEPVREDSSGMHHENQTYPPYSPQAQPQAIHRLDSPGLKPASQQKAEASSPVPYLSPETNPASSASAVNPNVSVTNVSLEEPAPAPPASHASQAGGLGAPGAEAAHVVLRGEGPPLPPHADPAQVYRKEPYPEEMMRQNHILKQPALGHPGQRPDKEPNLAYEPQLPYnullEKQASRDLEQPSYRYEASSYPDQFSRNYDHRLRFEDRIPTYEDQWSYYDDKQPYQPRPFENQHPRDLDSRQHnullEEASERGYnullQRFEEPAPLSYDSRTRYEQLPRTSTLRHEEQPAPAYEVHNRYRPEAQPYSSTGPKSSEPKQYFDQYPRSYEQVPPPGFTSKTGHYEPLHGAAVVPPLIPSSQHKPEVLPSATKPQPPPPTLTEEEEDPAMKPQSVLnullRVKMFENKRSASLENKKDVNDTASFKPPEVASKPPSASLAGPKPVPQSQFSEnullDKTLYRLPEPQKPQVKPPnullDIVRSNHYDPEEDEEYYRKQLSYFnullRRSFESKPSAHLPAGHHSEPAKPVHSQSQPNFSSYSSKGKPETDAVDRSFSEKRYDPAQATPPPPPLPSQYSQPAPPLSSSSLHIHSKGAQGEGNSVSLDFQNSYMSKPDPPPSQSKPATFRPPTREDPPQTFYPQKnullFPDKAPVnullGAEQTQKTITPVYNRFTPKPYTSSARPFERKFESPKFNHNLLPSETVHKPELSSKTPTSPKTLMKAHSSTQPPEFDSGVETFSVHTDnullPKYQMNNISTMPKAVPVSPSAVEEDEDEDGHTVVATARGIFNSNGGVLSSIETGVSIIIPQGAIPEGIEQEIYFKVCRDNSILPPLDKEKGETLLSPLVMCGPHGLKFLKPVELRLPHCDPKTWQNKCLPGDPNYLVGANCVSVLIDHF

Target ORF information:

RefSeq Version	XM_021166165.1
Organism	Mus caroli(Ryukyu mouse)
Definition	Mus caroli tight junction protein 1 (Tjp1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021166165.1

ORF Insert Sequence:

ATGTCCACCA GGGCCGCGGC CGCTAAGAGC ACAGCAATGG AGGAAACGGC TATATGGGAA 
CAGCACACAG TGATGCTTCA CAGGGCTCCT GGGTTTGGAT TTGGAATTGC AATCTCTGGT 
GGAAGAGATA ATCCTCATTT TCAGAGTGGG GAAACCTCCA TAGTGATTTC TGATGTGCTA 
AAAGGAGGGC CAGCTGAAGG ACAGCTACAG GAAAATGACC GAGTTGCAAT GGTTAATGGA 
GTTTCAATGG ATAACGTTGA ACATGCTTTT GCTGTTCAGC AGCTAAGGAA AAGTGGGAAA 
AACGCAAAAA TTACTATGCG AAGGAAGAAG AAAGTTCAGA TCCCTGTAAG TCACCCAGAT 
CCTGAGCCGG TGTCTGATAA TGAAGATGAT AGCTACGACG AAGAAGTACA TGATCCAAGA 
GCTGGCCGCA GTGCTTTAGC GAACAGAAGG AGCGAGAAGA GCTGGGCAAG GGATAGGAGT 
GCAAGCAGGG AGAGGAGCCT GTCCCCTCGC TCGGACAGGC GGTCGGTGGC CTCCAGTCAG 
CCCGCAAAAC CCACCAAGGT CGCACTGGTG AAGTCTCGGA AAAATGAAGA ATATGGTCTT 
CGATTGGCCA GCCATATATT TGTAAAGGAA ATTTCACAAG ATAGTTTGGC AGCAAGAGAT 
GGTAACATTC AAGAAGGGGA TGTTGTCTTG AAGATAAATG GTACTGTGAC AGAAAATATG 
TCATTGACAG ATGCAAAAAC ACTGATAGAA AGGTCTAAAG GCAAGTTAAA AATGGTAGTG 
CAAAGAGATG AGCGGGCTAC CTTACTGAAC GTCCCTGACC TTTCGGATAG TATCCATTCT 
GCTAATGCCT CGGAAAGAGA TGACATTTCA GAAATTCAGT CACTAGCATC AGACCATTCA 
GGTCGCTCGC ATGACAGGCC ACCCCGCCGC AGCCAGTCAC GATCTCCTGA CCAACGTTCA 
GAGCCCTCCG ATCATTCCAC ACAGTCTCCA CAGCAGCCCA GCAATGGCAG TCTCCGGAGC 
AGAGAGCAAG AGCGAATGTC TAAACCTGGG GCCATCTCAA CTCCTGTAAA ACATGCAGAT 
GATCATACAC CCAAAGCAGT GGAAGAAGTT ACAGTTGAGA AAAATGAGAA GCAGACACCC 
ACTCTTCCAG AACCGAAGCC TGTATATGCT CAAGTTGGAC AACCAGATGT GGATTTACCC 
GTCAGCCCTT CTGATGGTGT TCTGCCTAAT TCAACTCATG AAGATGGGAT ACTTAGGCCC 
AGCATGAAAC TGGTAAAATT CAGAAAAGGA GATAGTGTGG GTTTGCGACT AGCTGGTGGA 
AATGATGTCG GAATATTTGT AGCTGGCGTT CTAGAAGATA GCCCTGCAGC CAAAGAAGGC 
TTAGAGGAAG GTGATCAAAT TCTCAGGGTG AACAATGTAG ATTTCACAAA TATCATAAGA 
GAAGAGGCTG TCCTTTTCCT CCTTGACCTC CCTAAAGGTG AAGAAGTGAC CATCCTGGCT 
CAGAAGAAGA AGGGTGTTTA TCGCCGCATT GTAGAATCAG ATGTAGGAGA TTCATTCTAT 
ATTAGAACGC ATTTTGAGTA TGAAAAAGAA TCTCCTTACG GACTTAGTTT TAACAAAGGA 
GAGGTGTTCC GGGTCGTGGA TACTTTATAC AATGGAAAGC TGGGCTCTTG GCTTGCCATT 
CGAATTGGCA AAAATCATAA GGAGGTAGAA CGAGGCATCA TCCCTAATAA GAACAGAGCT 
GAGCAGTTAG CCAGTGTACA GTACACACTT CCAAAGACAG CGGGTGGTGA TCGGGCAGAC 
TTCTGGAGGT TTCGAGGTCT TCGCAGCTCC AAGAGAAATC TTCGAAAAAG CAGAGAGGAC 
TTGTCAGCTC AGCCAGTTCA AACTAAGTTC CCAGCTTATG AAAGGGTTGT TCTTCGAGAA 
GCTGGATTCC TAAGACCTGT AACCATCTTT GGACCAATAG CTGATGTTGC CAGAGAAAAG 
TTGGCAAGAG AGGAACCAGA TATCTATCAG ATTGCAAAAA GTGAACCACG AGATGCTGGG 
ACTGACCATC GTAGCTCTGG CATCATTCGC CTTCATACAA TAAAGCAAAT CATAGATCAG 
GATAAACATG CGTTATTAGA TGTAACGCCA AATGCGGTTG ATCGTCTTAA TTATGCGCAG 
TGGTATCCAA TTGTTGTGTT CCTTAACCCT GACTCTAAGC AAGGTGTAAA AACAATGAGG 
ATGAGGTTAT GTCCGGAGTC TCGGAAAAGC GCCAGGAAGC TATATGAACG CTCTCATAAG 
CTTCGCAAAA ACAATCACCA CCTTTTCACA ACTACAATTA ACTTAAACTC AATGAATGAT 
GGTTGGTACG GTGCCCTGAA AGAAGCCATT CAGCAGCAAC AGAACCAGCT GGTGTGGGTC 
TCTGAGGGGA AGGCGGATGG TGCTACAAGT GATGACCTTG ATTTGCATGA TGATCGTCTG 
TCCTACCTGT CAGCCCCAGG TAGTGAGTAC TCAATGTATA GCACGGACAG TAGACACACT 
TCTGACTATG AAGACACAGA TACAGAAGGC GGGGCCTACA CTGATCAAGA ACTAGATGAA 
ACTCTTAATG ATGAGGTGGG GACTCCCCCG GAGTCTGCCA TTACACNGTC CTCTGAGCCT 
GTAAGAGAGG ATTCCTCTGG GATGCATCAT GAAAACCAGA CCTATCCTCC TTACTCACCA 
CAAGCGCAGC CACAAGCTAT TCATAGACTA GACTCCCCTG GACTTAAGCC AGCCTCTCAA 
CAGAAAGCAG AAGCCTCATC TCCAGTCCCT TACCTTTCGC CTGAAACAAA CCCAGCATCG 
TCAGCCTCTG CAGTTAATCC TAATGTCAGT GTAACTAATG TCAGCCTGGA GGAGCCTGCC 
CCAGCCCCTC CCGCCTCGCA CGCATCCCAG GCTGGTGGTT TAGGAGCACC AGGTGCTGAG 
GCAGCTCACG TAGTGCTCAG AGGTGAAGGA CCACCATTGC CGCCGCATGC AGACCCAGCA 
CAGGTGTATA GAAAGGAGCC GTATCCTGAA GAAATGATGA GACAAAACCA TATTTTAAAA 
CAACCAGCTC TTGGTCACCC AGGGCAGAGG CCAGATAAAG AGCCAAATCT AGCCTATGAA 
CCCCAACTTC CATATANAGA AAAACAAGCC AGCAGAGACC TTGAACAGCC GTCATACAGG 
TATGAGGCCT CAAGCTACCC AGACCAGTTT TCTCGGAACT ATGACCATCG CCTACGGTTT 
GAAGATCGAA TCCCTACCTA TGAAGACCAG TGGTCATATT ATGATGACAA ACAGCCCTAC 
CAACCTCGGC CTTTTGAGAA TCAGCATCCC CGAGACCTGG ACTCCAGACA ACATCCNGAG 
GAGGCTTCAG AACGAGGTTA TNTCCAGCGT TTTGAAGAGC CAGCCCCTCT GTCATACGAC 
AGTAGAACAC GCTATGAGCA GCTGCCTCGA ACCTCTACTC TACGACATGA AGAGCAGCCA 
GCCCCTGCAT ATGAGGTGCA CAACAGGTAC AGGCCAGAGG CACAGCCCTA TTCTTCAACA 
GGTCCTAAGT CATCTGAGCC CAAGCAGTAC TTTGACCAGT ACCCGCGAAG TTATGAGCAA 
GTACCACCAC CAGGCTTTAC CTCCAAAACA GGCCATTACG AGCCTCTCCA TGGTGCTGCA 
GTTGTCCCTC CTCTGATACC TTCCTCTCAA CATAAGCCAG AAGTCCTGCC CTCGGCTACC 
AAACCACAGC CTCCACCCCC AACCCTAACT GAGGAGGAGG AGGATCCAGC AATGAAACCA 
CAGTCCGTGC TCANCAGAGT CAAAATGTTT GAAAACAAAA GATCTGCGTC TTTGGAGAAC 
AAGAAAGATG TGAATGACAC TGCCAGCTTT AAGCCTCCAG AAGTAGCATC TAAACCTCCA 
AGTGCTTCTC TTGCTGGCCC TAAACCTGTT CCTCAGAGTC AGTTTAGTGA GCANGACAAA 
ACACTCTACA GGCTCCCAGA GCCTCAGAAA CCTCAAGTGA AGCCACCCGA NGATATTGTT 
CGATCAAATC ATTACGACCC TGAAGAGGAT GAAGAATATT ACAGGAAACA GCTCTCTTAC 
TTTGANCGAA GAAGTTTTGA GAGCAAGCCT TCTGCACATC TTCCTGCTGG CCATCACTCA 
GAGCCTGCTA AGCCAGTCCA TTCTCAGAGC CAGCCCAACT TTTCTAGTTA CTCTTCAAAG 
GGAAAACCCG AAACTGATGC TGTGGATAGA TCATTCAGTG AGAAACGTTA TGATCCAGCC 
CAGGCCACGC CTCCTCCTCC TCCGTTGCCC TCACAGTACA GCCAGCCAGC TCCCCCTCTG 
TCCAGCTCTT CTCTCCACAT ACATTCCAAG GGCGCCCAGG GCGAAGGCAA TTCAGTATCA 
TTGGATTTTC AGAACTCATA TATGTCCAAA CCAGACCCAC CCCCATCTCA GAGTAAACCA 
GCAACTTTCA GACCACCAAC CCGAGAAGAC CCTCCTCAGA CTTTCTATCC CCAGAAANGT 
TTCCCAGACA AAGCTCCAGT TAANGGAGCT GAGCAGACTC AGAAAACCAT CACTCCAGTG 
TACAATCGAT TCACACCAAA GCCGTACACA AGTTCTGCCC GGCCGTTTGA ACGCAAATTT 
GAAAGTCCGA AGTTCAACCA TAATCTTCTG CCAAGTGAAA CTGTACATAA ACCTGAATTG 
TCTTCAAAAA CTCCCACTTC TCCAAAAACT CTTATGAAAG CTCATAGTTC AACACAGCCT 
CCAGAGTTTG ACAGTGGAGT TGAAACTTTC TCTGTTCACA CAGATNAGCC TAAATATCAA 
ATGAATAATA TCAGCACCAT GCCTAAAGCT GTCCCTGTGA GTCCTTCAGC TGTGGAAGAA 
GATGAAGATG AGGATGGTCA TACTGTAGTG GCTACAGCTC GTGGCATTTT TAACAGCAAT 
GGTGGTGTGT TGAGTTCCAT AGAAACCGGT GTTAGTATAA TTATCCCACA AGGAGCCATT 
CCTGAAGGAA TTGAACAAGA AATCTATTTC AAAGTCTGCA GAGACAATAG CATTCTCCCA 
CCTTTAGATA AAGAGAAAGG TGAAACTCTG CTAAGTCCCC TAGTGATGTG TGGGCCCCAT 
GGCCTCAAGT TCCTGAAGCC CGTGGAGCTG CGCTTGCCAC ACTGTGACCC TAAAACCTGG 
CAAAACAAGT GTCTTCCTGG AGATCCGAAT TACCTTGTTG GAGCCAACTG TGTTTCTGTC 
CTGATTGACC ACTTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Tjp1 ( XM_021166165.2 ) cDNA ORF clone, Mus caroli(Ryukyu mouse) -> XP_021021824.1 Mus caroli tight junction protein 1 (Tjp1), transcript variant X8, mRNA.

Price & Availability↑

CloneID	OMv03979
Clone ID Related Accession (Same CDS sequence)	XM_021166165.1 , XM_021166165.2
Accession Version	XM_021166165.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5238bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-06-09
Organism	Mus caroli(Ryukyu mouse)
Product	tight junction protein ZO-1 isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_034576.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process On Jun 10, 2019 this sequence version replaced XM_021166165.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus caroli Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCACCA GGGCCGCGGC CGCTAAGAGC ACAGCAATGG AGGAAACGGC TATATGGGAA 
CAGCACACAG TGATGCTTCA CAGGGCTCCT GGGTTTGGAT TTGGAATTGC AATCTCTGGT 
GGAAGAGATA ATCCTCATTT TCAGAGTGGG GAAACCTCCA TAGTGATTTC TGATGTGCTA 
AAAGGAGGGC CAGCTGAAGG ACAGCTACAG GAAAATGACC GAGTTGCAAT GGTTAATGGA 
GTTTCAATGG ATAACGTTGA ACATGCTTTT GCTGTTCAGC AGCTAAGGAA AAGTGGGAAA 
AACGCAAAAA TTACTATGCG AAGGAAGAAG AAAGTTCAGA TCCCTGTAAG TCACCCAGAT 
CCTGAGCCGG TGTCTGATAA TGAAGATGAT AGCTACGACG AAGAAGTACA TGATCCAAGA 
GCTGGCCGCA GTGCTTTAGC GAACAGAAGG AGCGAGAAGA GCTGGGCAAG GGATAGGAGT 
GCAAGCAGGG AGAGGAGCCT GTCCCCTCGC TCGGACAGGC GGTCGGTGGC CTCCAGTCAG 
CCCGCAAAAC CCACCAAGGT CGCACTGGTG AAGTCTCGGA AAAATGAAGA ATATGGTCTT 
CGATTGGCCA GCCATATATT TGTAAAGGAA ATTTCACAAG ATAGTTTGGC AGCAAGAGAT 
GGTAACATTC AAGAAGGGGA TGTTGTCTTG AAGATAAATG GTACTGTGAC AGAAAATATG 
TCATTGACAG ATGCAAAAAC ACTGATAGAA AGGTCTAAAG GCAAGTTAAA AATGGTAGTG 
CAAAGAGATG AGCGGGCTAC CTTACTGAAC GTCCCTGACC TTTCGGATAG TATCCATTCT 
GCTAATGCCT CGGAAAGAGA TGACATTTCA GAAATTCAGT CACTAGCATC AGACCATTCA 
GGTCGCTCGC ATGACAGGCC ACCCCGCCGC AGCCAGTCAC GATCTCCTGA CCAACGTTCA 
GAGCCCTCCG ATCATTCCAC ACAGTCTCCA CAGCAGCCCA GCAATGGCAG TCTCCGGAGC 
AGAGAGCAAG AGCGAATGTC TAAACCTGGG GCCATCTCAA CTCCTGTAAA ACATGCAGAT 
GATCATACAC CCAAAGCAGT GGAAGAAGTT ACAGTTGAGA AAAATGAGAA GCAGACACCC 
ACTCTTCCAG AACCGAAGCC TGTATATGCT CAAGTTGGAC AACCAGATGT GGATTTACCC 
GTCAGCCCTT CTGATGGTGT TCTGCCTAAT TCAACTCATG AAGATGGGAT ACTTAGGCCC 
AGCATGAAAC TGGTAAAATT CAGAAAAGGA GATAGTGTGG GTTTGCGACT AGCTGGTGGA 
AATGATGTCG GAATATTTGT AGCTGGCGTT CTAGAAGATA GCCCTGCAGC CAAAGAAGGC 
TTAGAGGAAG GTGATCAAAT TCTCAGGGTG AACAATGTAG ATTTCACAAA TATCATAAGA 
GAAGAGGCTG TCCTTTTCCT CCTTGACCTC CCTAAAGGTG AAGAAGTGAC CATCCTGGCT 
CAGAAGAAGA AGGGTGTTTA TCGCCGCATT GTAGAATCAG ATGTAGGAGA TTCATTCTAT 
ATTAGAACGC ATTTTGAGTA TGAAAAAGAA TCTCCTTACG GACTTAGTTT TAACAAAGGA 
GAGGTGTTCC GGGTCGTGGA TACTTTATAC AATGGAAAGC TGGGCTCTTG GCTTGCCATT 
CGAATTGGCA AAAATCATAA GGAGGTAGAA CGAGGCATCA TCCCTAATAA GAACAGAGCT 
GAGCAGTTAG CCAGTGTACA GTACACACTT CCAAAGACAG CGGGTGGTGA TCGGGCAGAC 
TTCTGGAGGT TTCGAGGTCT TCGCAGCTCC AAGAGAAATC TTCGAAAAAG CAGAGAGGAC 
TTGTCAGCTC AGCCAGTTCA AACTAAGTTC CCAGCTTATG AAAGGGTTGT TCTTCGAGAA 
GCTGGATTCC TAAGACCTGT AACCATCTTT GGACCAATAG CTGATGTTGC CAGAGAAAAG 
TTGGCAAGAG AGGAACCAGA TATCTATCAG ATTGCAAAAA GTGAACCACG AGATGCTGGG 
ACTGACCATC GTAGCTCTGG CATCATTCGC CTTCATACAA TAAAGCAAAT CATAGATCAG 
GATAAACATG CGTTATTAGA TGTAACGCCA AATGCGGTTG ATCGTCTTAA TTATGCGCAG 
TGGTATCCAA TTGTTGTGTT CCTTAACCCT GACTCTAAGC AAGGTGTAAA AACAATGAGG 
ATGAGGTTAT GTCCGGAGTC TCGGAAAAGC GCCAGGAAGC TATATGAACG CTCTCATAAG 
CTTCGCAAAA ACAATCACCA CCTTTTCACA ACTACAATTA ACTTAAACTC AATGAATGAT 
GGTTGGTACG GTGCCCTGAA AGAAGCCATT CAGCAGCAAC AGAACCAGCT GGTGTGGGTC 
TCTGAGGGGA AGGCGGATGG TGCTACAAGT GATGACCTTG ATTTGCATGA TGATCGTCTG 
TCCTACCTGT CAGCCCCAGG TAGTGAGTAC TCAATGTATA GCACGGACAG TAGACACACT 
TCTGACTATG AAGACACAGA TACAGAAGGC GGGGCCTACA CTGATCAAGA ACTAGATGAA 
ACTCTTAATG ATGAGGTGGG GACTCCCCCG GAGTCTGCCA TTACACNGTC CTCTGAGCCT 
GTAAGAGAGG ATTCCTCTGG GATGCATCAT GAAAACCAGA CCTATCCTCC TTACTCACCA 
CAAGCGCAGC CACAAGCTAT TCATAGACTA GACTCCCCTG GACTTAAGCC AGCCTCTCAA 
CAGAAAGCAG AAGCCTCATC TCCAGTCCCT TACCTTTCGC CTGAAACAAA CCCAGCATCG 
TCAGCCTCTG CAGTTAATCC TAATGTCAGT GTAACTAATG TCAGCCTGGA GGAGCCTGCC 
CCAGCCCCTC CCGCCTCGCA CGCATCCCAG GCTGGTGGTT TAGGAGCACC AGGTGCTGAG 
GCAGCTCACG TAGTGCTCAG AGGTGAAGGA CCACCATTGC CGCCGCATGC AGACCCAGCA 
CAGGTGTATA GAAAGGAGCC GTATCCTGAA GAAATGATGA GACAAAACCA TATTTTAAAA 
CAACCAGCTC TTGGTCACCC AGGGCAGAGG CCAGATAAAG AGCCAAATCT AGCCTATGAA 
CCCCAACTTC CATATANAGA AAAACAAGCC AGCAGAGACC TTGAACAGCC GTCATACAGG 
TATGAGGCCT CAAGCTACCC AGACCAGTTT TCTCGGAACT ATGACCATCG CCTACGGTTT 
GAAGATCGAA TCCCTACCTA TGAAGACCAG TGGTCATATT ATGATGACAA ACAGCCCTAC 
CAACCTCGGC CTTTTGAGAA TCAGCATCCC CGAGACCTGG ACTCCAGACA ACATCCNGAG 
GAGGCTTCAG AACGAGGTTA TNTCCAGCGT TTTGAAGAGC CAGCCCCTCT GTCATACGAC 
AGTAGAACAC GCTATGAGCA GCTGCCTCGA ACCTCTACTC TACGACATGA AGAGCAGCCA 
GCCCCTGCAT ATGAGGTGCA CAACAGGTAC AGGCCAGAGG CACAGCCCTA TTCTTCAACA 
GGTCCTAAGT CATCTGAGCC CAAGCAGTAC TTTGACCAGT ACCCGCGAAG TTATGAGCAA 
GTACCACCAC CAGGCTTTAC CTCCAAAACA GGCCATTACG AGCCTCTCCA TGGTGCTGCA 
GTTGTCCCTC CTCTGATACC TTCCTCTCAA CATAAGCCAG AAGTCCTGCC CTCGGCTACC 
AAACCACAGC CTCCACCCCC AACCCTAACT GAGGAGGAGG AGGATCCAGC AATGAAACCA 
CAGTCCGTGC TCANCAGAGT CAAAATGTTT GAAAACAAAA GATCTGCGTC TTTGGAGAAC 
AAGAAAGATG TGAATGACAC TGCCAGCTTT AAGCCTCCAG AAGTAGCATC TAAACCTCCA 
AGTGCTTCTC TTGCTGGCCC TAAACCTGTT CCTCAGAGTC AGTTTAGTGA GCANGACAAA 
ACACTCTACA GGCTCCCAGA GCCTCAGAAA CCTCAAGTGA AGCCACCCGA NGATATTGTT 
CGATCAAATC ATTACGACCC TGAAGAGGAT GAAGAATATT ACAGGAAACA GCTCTCTTAC 
TTTGANCGAA GAAGTTTTGA GAGCAAGCCT TCTGCACATC TTCCTGCTGG CCATCACTCA 
GAGCCTGCTA AGCCAGTCCA TTCTCAGAGC CAGCCCAACT TTTCTAGTTA CTCTTCAAAG 
GGAAAACCCG AAACTGATGC TGTGGATAGA TCATTCAGTG AGAAACGTTA TGATCCAGCC 
CAGGCCACGC CTCCTCCTCC TCCGTTGCCC TCACAGTACA GCCAGCCAGC TCCCCCTCTG 
TCCAGCTCTT CTCTCCACAT ACATTCCAAG GGCGCCCAGG GCGAAGGCAA TTCAGTATCA 
TTGGATTTTC AGAACTCATA TATGTCCAAA CCAGACCCAC CCCCATCTCA GAGTAAACCA 
GCAACTTTCA GACCACCAAC CCGAGAAGAC CCTCCTCAGA CTTTCTATCC CCAGAAANGT 
TTCCCAGACA AAGCTCCAGT TAANGGAGCT GAGCAGACTC AGAAAACCAT CACTCCAGTG 
TACAATCGAT TCACACCAAA GCCGTACACA AGTTCTGCCC GGCCGTTTGA ACGCAAATTT 
GAAAGTCCGA AGTTCAACCA TAATCTTCTG CCAAGTGAAA CTGTACATAA ACCTGAATTG 
TCTTCAAAAA CTCCCACTTC TCCAAAAACT CTTATGAAAG CTCATAGTTC AACACAGCCT 
CCAGAGTTTG ACAGTGGAGT TGAAACTTTC TCTGTTCACA CAGATNAGCC TAAATATCAA 
ATGAATAATA TCAGCACCAT GCCTAAAGCT GTCCCTGTGA GTCCTTCAGC TGTGGAAGAA 
GATGAAGATG AGGATGGTCA TACTGTAGTG GCTACAGCTC GTGGCATTTT TAACAGCAAT 
GGTGGTGTGT TGAGTTCCAT AGAAACCGGT GTTAGTATAA TTATCCCACA AGGAGCCATT 
CCTGAAGGAA TTGAACAAGA AATCTATTTC AAAGTCTGCA GAGACAATAG CATTCTCCCA 
CCTTTAGATA AAGAGAAAGG TGAAACTCTG CTAAGTCCCC TAGTGATGTG TGGGCCCCAT 
GGCCTCAAGT TCCTGAAGCC CGTGGAGCTG CGCTTGCCAC ACTGTGACCC TAAAACCTGG 
CAAAACAAGT GTCTTCCTGG AGATCCGAAT TACCTTGTTG GAGCCAACTG TGTTTCTGTC 
CTGATTGACC ACTTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021021824.1
CDS	65..5302
Translation	MSTRAAAAKSTAMEETAIWEQHTVMLHRAPGFGFGIAISGGRDNPHFQSGETSIVISDVLKGGPAEGQLQENDRVAMVNGVSMDNVEHAFAVQQLRKSGKNAKITMRRKKKVQIPVSHPDPEPVSDNEDDSYDEEVHDPRAGRSALANRRSEKSWARDRSASRERSLSPRSDRRSVASSQPAKPTKVALVKSRKNEEYGLRLASHIFVKEISQDSLAARDGNIQEGDVVLKINGTVTENMSLTDAKTLIERSKGKLKMVVQRDERATLLNVPDLSDSIHSANASERDDISEIQSLASDHSGRSHDRPPRRSQSRSPDQRSEPSDHSTQSPQQPSNGSLRSREQERMSKPGAISTPVKHADDHTPKAVEEVTVEKNEKQTPTLPEPKPVYAQVGQPDVDLPVSPSDGVLPNSTHEDGILRPSMKLVKFRKGDSVGLRLAGGNDVGIFVAGVLEDSPAAKEGLEEGDQILRVNNVDFTNIIREEAVLFLLDLPKGEEVTILAQKKKGVYRRIVESDVGDSFYIRTHFEYEKESPYGLSFNKGEVFRVVDTLYNGKLGSWLAIRIGKNHKEVERGIIPNKNRAEQLASVQYTLPKTAGGDRADFWRFRGLRSSKRNLRKSREDLSAQPVQTKFPAYERVVLREAGFLRPVTIFGPIADVAREKLAREEPDIYQIAKSEPRDAGTDHRSSGIIRLHTIKQIIDQDKHALLDVTPNAVDRLNYAQWYPIVVFLNPDSKQGVKTMRMRLCPESRKSARKLYERSHKLRKNNHHLFTTTINLNSMNDGWYGALKEAIQQQQNQLVWVSEGKADGATSDDLDLHDDRLSYLSAPGSEYSMYSTDSRHTSDYEDTDTEGGAYTDQELDETLNDEVGTPPESAITnullSSEPVREDSSGMHHENQTYPPYSPQAQPQAIHRLDSPGLKPASQQKAEASSPVPYLSPETNPASSASAVNPNVSVTNVSLEEPAPAPPASHASQAGGLGAPGAEAAHVVLRGEGPPLPPHADPAQVYRKEPYPEEMMRQNHILKQPALGHPGQRPDKEPNLAYEPQLPYnullEKQASRDLEQPSYRYEASSYPDQFSRNYDHRLRFEDRIPTYEDQWSYYDDKQPYQPRPFENQHPRDLDSRQHnullEEASERGYnullQRFEEPAPLSYDSRTRYEQLPRTSTLRHEEQPAPAYEVHNRYRPEAQPYSSTGPKSSEPKQYFDQYPRSYEQVPPPGFTSKTGHYEPLHGAAVVPPLIPSSQHKPEVLPSATKPQPPPPTLTEEEEDPAMKPQSVLnullRVKMFENKRSASLENKKDVNDTASFKPPEVASKPPSASLAGPKPVPQSQFSEnullDKTLYRLPEPQKPQVKPPnullDIVRSNHYDPEEDEEYYRKQLSYFnullRRSFESKPSAHLPAGHHSEPAKPVHSQSQPNFSSYSSKGKPETDAVDRSFSEKRYDPAQATPPPPPLPSQYSQPAPPLSSSSLHIHSKGAQGEGNSVSLDFQNSYMSKPDPPPSQSKPATFRPPTREDPPQTFYPQKnullFPDKAPVnullGAEQTQKTITPVYNRFTPKPYTSSARPFERKFESPKFNHNLLPSETVHKPELSSKTPTSPKTLMKAHSSTQPPEFDSGVETFSVHTDnullPKYQMNNISTMPKAVPVSPSAVEEDEDEDGHTVVATARGIFNSNGGVLSSIETGVSIIIPQGAIPEGIEQEIYFKVCRDNSILPPLDKEKGETLLSPLVMCGPHGLKFLKPVELRLPHCDPKTWQNKCLPGDPNYLVGANCVSVLIDHF

Target ORF information:

RefSeq Version	XM_021166165.2
Organism	Mus caroli(Ryukyu mouse)
Definition	Mus caroli tight junction protein 1 (Tjp1), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021166165.2

ORF Insert Sequence:

ATGTCCACCA GGGCCGCGGC CGCTAAGAGC ACAGCAATGG AGGAAACGGC TATATGGGAA 
CAGCACACAG TGATGCTTCA CAGGGCTCCT GGGTTTGGAT TTGGAATTGC AATCTCTGGT 
GGAAGAGATA ATCCTCATTT TCAGAGTGGG GAAACCTCCA TAGTGATTTC TGATGTGCTA 
AAAGGAGGGC CAGCTGAAGG ACAGCTACAG GAAAATGACC GAGTTGCAAT GGTTAATGGA 
GTTTCAATGG ATAACGTTGA ACATGCTTTT GCTGTTCAGC AGCTAAGGAA AAGTGGGAAA 
AACGCAAAAA TTACTATGCG AAGGAAGAAG AAAGTTCAGA TCCCTGTAAG TCACCCAGAT 
CCTGAGCCGG TGTCTGATAA TGAAGATGAT AGCTACGACG AAGAAGTACA TGATCCAAGA 
GCTGGCCGCA GTGCTTTAGC GAACAGAAGG AGCGAGAAGA GCTGGGCAAG GGATAGGAGT 
GCAAGCAGGG AGAGGAGCCT GTCCCCTCGC TCGGACAGGC GGTCGGTGGC CTCCAGTCAG 
CCCGCAAAAC CCACCAAGGT CGCACTGGTG AAGTCTCGGA AAAATGAAGA ATATGGTCTT 
CGATTGGCCA GCCATATATT TGTAAAGGAA ATTTCACAAG ATAGTTTGGC AGCAAGAGAT 
GGTAACATTC AAGAAGGGGA TGTTGTCTTG AAGATAAATG GTACTGTGAC AGAAAATATG 
TCATTGACAG ATGCAAAAAC ACTGATAGAA AGGTCTAAAG GCAAGTTAAA AATGGTAGTG 
CAAAGAGATG AGCGGGCTAC CTTACTGAAC GTCCCTGACC TTTCGGATAG TATCCATTCT 
GCTAATGCCT CGGAAAGAGA TGACATTTCA GAAATTCAGT CACTAGCATC AGACCATTCA 
GGTCGCTCGC ATGACAGGCC ACCCCGCCGC AGCCAGTCAC GATCTCCTGA CCAACGTTCA 
GAGCCCTCCG ATCATTCCAC ACAGTCTCCA CAGCAGCCCA GCAATGGCAG TCTCCGGAGC 
AGAGAGCAAG AGCGAATGTC TAAACCTGGG GCCATCTCAA CTCCTGTAAA ACATGCAGAT 
GATCATACAC CCAAAGCAGT GGAAGAAGTT ACAGTTGAGA AAAATGAGAA GCAGACACCC 
ACTCTTCCAG AACCGAAGCC TGTATATGCT CAAGTTGGAC AACCAGATGT GGATTTACCC 
GTCAGCCCTT CTGATGGTGT TCTGCCTAAT TCAACTCATG AAGATGGGAT ACTTAGGCCC 
AGCATGAAAC TGGTAAAATT CAGAAAAGGA GATAGTGTGG GTTTGCGACT AGCTGGTGGA 
AATGATGTCG GAATATTTGT AGCTGGCGTT CTAGAAGATA GCCCTGCAGC CAAAGAAGGC 
TTAGAGGAAG GTGATCAAAT TCTCAGGGTG AACAATGTAG ATTTCACAAA TATCATAAGA 
GAAGAGGCTG TCCTTTTCCT CCTTGACCTC CCTAAAGGTG AAGAAGTGAC CATCCTGGCT 
CAGAAGAAGA AGGGTGTTTA TCGCCGCATT GTAGAATCAG ATGTAGGAGA TTCATTCTAT 
ATTAGAACGC ATTTTGAGTA TGAAAAAGAA TCTCCTTACG GACTTAGTTT TAACAAAGGA 
GAGGTGTTCC GGGTCGTGGA TACTTTATAC AATGGAAAGC TGGGCTCTTG GCTTGCCATT 
CGAATTGGCA AAAATCATAA GGAGGTAGAA CGAGGCATCA TCCCTAATAA GAACAGAGCT 
GAGCAGTTAG CCAGTGTACA GTACACACTT CCAAAGACAG CGGGTGGTGA TCGGGCAGAC 
TTCTGGAGGT TTCGAGGTCT TCGCAGCTCC AAGAGAAATC TTCGAAAAAG CAGAGAGGAC 
TTGTCAGCTC AGCCAGTTCA AACTAAGTTC CCAGCTTATG AAAGGGTTGT TCTTCGAGAA 
GCTGGATTCC TAAGACCTGT AACCATCTTT GGACCAATAG CTGATGTTGC CAGAGAAAAG 
TTGGCAAGAG AGGAACCAGA TATCTATCAG ATTGCAAAAA GTGAACCACG AGATGCTGGG 
ACTGACCATC GTAGCTCTGG CATCATTCGC CTTCATACAA TAAAGCAAAT CATAGATCAG 
GATAAACATG CGTTATTAGA TGTAACGCCA AATGCGGTTG ATCGTCTTAA TTATGCGCAG 
TGGTATCCAA TTGTTGTGTT CCTTAACCCT GACTCTAAGC AAGGTGTAAA AACAATGAGG 
ATGAGGTTAT GTCCGGAGTC TCGGAAAAGC GCCAGGAAGC TATATGAACG CTCTCATAAG 
CTTCGCAAAA ACAATCACCA CCTTTTCACA ACTACAATTA ACTTAAACTC AATGAATGAT 
GGTTGGTACG GTGCCCTGAA AGAAGCCATT CAGCAGCAAC AGAACCAGCT GGTGTGGGTC 
TCTGAGGGGA AGGCGGATGG TGCTACAAGT GATGACCTTG ATTTGCATGA TGATCGTCTG 
TCCTACCTGT CAGCCCCAGG TAGTGAGTAC TCAATGTATA GCACGGACAG TAGACACACT 
TCTGACTATG AAGACACAGA TACAGAAGGC GGGGCCTACA CTGATCAAGA ACTAGATGAA 
ACTCTTAATG ATGAGGTGGG GACTCCCCCG GAGTCTGCCA TTACACNGTC CTCTGAGCCT 
GTAAGAGAGG ATTCCTCTGG GATGCATCAT GAAAACCAGA CCTATCCTCC TTACTCACCA 
CAAGCGCAGC CACAAGCTAT TCATAGACTA GACTCCCCTG GACTTAAGCC AGCCTCTCAA 
CAGAAAGCAG AAGCCTCATC TCCAGTCCCT TACCTTTCGC CTGAAACAAA CCCAGCATCG 
TCAGCCTCTG CAGTTAATCC TAATGTCAGT GTAACTAATG TCAGCCTGGA GGAGCCTGCC 
CCAGCCCCTC CCGCCTCGCA CGCATCCCAG GCTGGTGGTT TAGGAGCACC AGGTGCTGAG 
GCAGCTCACG TAGTGCTCAG AGGTGAAGGA CCACCATTGC CGCCGCATGC AGACCCAGCA 
CAGGTGTATA GAAAGGAGCC GTATCCTGAA GAAATGATGA GACAAAACCA TATTTTAAAA 
CAACCAGCTC TTGGTCACCC AGGGCAGAGG CCAGATAAAG AGCCAAATCT AGCCTATGAA 
CCCCAACTTC CATATANAGA AAAACAAGCC AGCAGAGACC TTGAACAGCC GTCATACAGG 
TATGAGGCCT CAAGCTACCC AGACCAGTTT TCTCGGAACT ATGACCATCG CCTACGGTTT 
GAAGATCGAA TCCCTACCTA TGAAGACCAG TGGTCATATT ATGATGACAA ACAGCCCTAC 
CAACCTCGGC CTTTTGAGAA TCAGCATCCC CGAGACCTGG ACTCCAGACA ACATCCNGAG 
GAGGCTTCAG AACGAGGTTA TNTCCAGCGT TTTGAAGAGC CAGCCCCTCT GTCATACGAC 
AGTAGAACAC GCTATGAGCA GCTGCCTCGA ACCTCTACTC TACGACATGA AGAGCAGCCA 
GCCCCTGCAT ATGAGGTGCA CAACAGGTAC AGGCCAGAGG CACAGCCCTA TTCTTCAACA 
GGTCCTAAGT CATCTGAGCC CAAGCAGTAC TTTGACCAGT ACCCGCGAAG TTATGAGCAA 
GTACCACCAC CAGGCTTTAC CTCCAAAACA GGCCATTACG AGCCTCTCCA TGGTGCTGCA 
GTTGTCCCTC CTCTGATACC TTCCTCTCAA CATAAGCCAG AAGTCCTGCC CTCGGCTACC 
AAACCACAGC CTCCACCCCC AACCCTAACT GAGGAGGAGG AGGATCCAGC AATGAAACCA 
CAGTCCGTGC TCANCAGAGT CAAAATGTTT GAAAACAAAA GATCTGCGTC TTTGGAGAAC 
AAGAAAGATG TGAATGACAC TGCCAGCTTT AAGCCTCCAG AAGTAGCATC TAAACCTCCA 
AGTGCTTCTC TTGCTGGCCC TAAACCTGTT CCTCAGAGTC AGTTTAGTGA GCANGACAAA 
ACACTCTACA GGCTCCCAGA GCCTCAGAAA CCTCAAGTGA AGCCACCCGA NGATATTGTT 
CGATCAAATC ATTACGACCC TGAAGAGGAT GAAGAATATT ACAGGAAACA GCTCTCTTAC 
TTTGANCGAA GAAGTTTTGA GAGCAAGCCT TCTGCACATC TTCCTGCTGG CCATCACTCA 
GAGCCTGCTA AGCCAGTCCA TTCTCAGAGC CAGCCCAACT TTTCTAGTTA CTCTTCAAAG 
GGAAAACCCG AAACTGATGC TGTGGATAGA TCATTCAGTG AGAAACGTTA TGATCCAGCC 
CAGGCCACGC CTCCTCCTCC TCCGTTGCCC TCACAGTACA GCCAGCCAGC TCCCCCTCTG 
TCCAGCTCTT CTCTCCACAT ACATTCCAAG GGCGCCCAGG GCGAAGGCAA TTCAGTATCA 
TTGGATTTTC AGAACTCATA TATGTCCAAA CCAGACCCAC CCCCATCTCA GAGTAAACCA 
GCAACTTTCA GACCACCAAC CCGAGAAGAC CCTCCTCAGA CTTTCTATCC CCAGAAANGT 
TTCCCAGACA AAGCTCCAGT TAANGGAGCT GAGCAGACTC AGAAAACCAT CACTCCAGTG 
TACAATCGAT TCACACCAAA GCCGTACACA AGTTCTGCCC GGCCGTTTGA ACGCAAATTT 
GAAAGTCCGA AGTTCAACCA TAATCTTCTG CCAAGTGAAA CTGTACATAA ACCTGAATTG 
TCTTCAAAAA CTCCCACTTC TCCAAAAACT CTTATGAAAG CTCATAGTTC AACACAGCCT 
CCAGAGTTTG ACAGTGGAGT TGAAACTTTC TCTGTTCACA CAGATNAGCC TAAATATCAA 
ATGAATAATA TCAGCACCAT GCCTAAAGCT GTCCCTGTGA GTCCTTCAGC TGTGGAAGAA 
GATGAAGATG AGGATGGTCA TACTGTAGTG GCTACAGCTC GTGGCATTTT TAACAGCAAT 
GGTGGTGTGT TGAGTTCCAT AGAAACCGGT GTTAGTATAA TTATCCCACA AGGAGCCATT 
CCTGAAGGAA TTGAACAAGA AATCTATTTC AAAGTCTGCA GAGACAATAG CATTCTCCCA 
CCTTTAGATA AAGAGAAAGG TGAAACTCTG CTAAGTCCCC TAGTGATGTG TGGGCCCCAT 
GGCCTCAAGT TCCTGAAGCC CGTGGAGCTG CGCTTGCCAC ACTGTGACCC TAAAACCTGG 
CAAAACAAGT GTCTTCCTGG AGATCCGAAT TACCTTGTTG GAGCCAACTG TGTTTCTGTC 
CTGATTGACC ACTTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Tjp1 ( XM_021166166.1 ) cDNA ORF clone, Mus caroli(Ryukyu mouse) -> XP_021021825.1 Mus caroli tight junction protein 1 (Tjp1), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OMv03980
Clone ID Related Accession (Same CDS sequence)	XM_021166166.1 , XM_021166166.2
Accession Version	XM_021166166.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5058bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-05-20
Organism	Mus caroli(Ryukyu mouse)
Product	tight junction protein ZO-1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_034576.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus caroli Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCACCA GGGCCGCGGC CGCTAAGAGC ACAGCAATGG AGGAAACGGC TATATGGGAA 
CAGCACACAG TGATGCTTCA CAGGGCTCCT GGGTTTGGAT TTGGAATTGC AATCTCTGGT 
GGAAGAGATA ATCCTCATTT TCAGAGTGGG GAAACCTCCA TAGTGATTTC TGATGTGCTA 
AAAGGAGGGC CAGCTGAAGG ACAGCTACAG GAAAATGACC GAGTTGCAAT GGTTAATGGA 
GTTTCAATGG ATAACGTTGA ACATGCTTTT GCTGTTCAGC AGCTAAGGAA AAGTGGGAAA 
AACGCAAAAA TTACTATGCG AAGGAAGAAG AAAGTTCAGA TCCCTGTAAG TCACCCAGAT 
CCTGAGCCGG TGTCTGATAA TGAAGATGAT AGCTACGACG AAGAAGTACA TGATCCAAGA 
GCTGGCCGCA GTGCTTTAGC GAACAGAAGG AGCGAGAAGA GCTGGGCAAG GGATAGGAGT 
GCAAGCAGGG AGAGGAGCCT GTCCCCTCGC TCGGACAGGC GGTCGGTGGC CTCCAGTCAG 
CCCGCAAAAC CCACCAAGGT CGCACTGGTG AAGTCTCGGA AAAATGAAGA ATATGGTCTT 
CGATTGGCCA GCCATATATT TGTAAAGGAA ATTTCACAAG ATAGTTTGGC AGCAAGAGAT 
GGTAACATTC AAGAAGGGGA TGTTGTCTTG AAGATAAATG GTACTGTGAC AGAAAATATG 
TCATTGACAG ATGCAAAAAC ACTGATAGAA AGGTCTAAAG GCAAGTTAAA AATGGTAGTG 
CAAAGAGATG AGCGGGCTAC CTTACTGAAC GTCCCTGACC TTTCGGATAG TATCCATTCT 
GCTAATGCCT CGGAAAGAGA TGACATTTCA GAAATTCAGT CACTAGCATC AGACCATTCA 
GGTCGCTCGC ATGACAGGCC ACCCCGCCGC AGCCAGTCAC GATCTCCTGA CCAACGTTCA 
GAGCCCTCCG ATCATTCCAC ACAGTCTCCA CAGCAGCCCA GCAATGGCAG TCTCCGGAGC 
AGAGAGCAAG AGCGAATGTC TAAACCTGGG GCCATCTCAA CTCCTGTAAA ACATGCAGAT 
GATCATACAC CCAAAGCAGT GGAAGAAGTT ACAGTTGAGA AAAATGAGAA GCAGACACCC 
ACTCTTCCAG AACCGAAGCC TGTATATGCT CAAGTTGGAC AACCAGATGT GGATTTACCC 
GTCAGCCCTT CTGATGGTGT TCTGCCTAAT TCAACTCATG AAGATGGGAT ACTTAGGCCC 
AGCATGAAAC TGGTAAAATT CAGAAAAGGA GATAGTGTGG GTTTGCGACT AGCTGGTGGA 
AATGATGTCG GAATATTTGT AGCTGGCGTT CTAGAAGATA GCCCTGCAGC CAAAGAAGGC 
TTAGAGGAAG GTGATCAAAT TCTCAGGGTG AACAATGTAG ATTTCACAAA TATCATAAGA 
GAAGAGGCTG TCCTTTTCCT CCTTGACCTC CCTAAAGGTG AAGAAGTGAC CATCCTGGCT 
CAGAAGAAGA AGGGTGTTTA TCGCCGCATT GTAGAATCAG ATGTAGGAGA TTCATTCTAT 
ATTAGAACGC ATTTTGAGTA TGAAAAAGAA TCTCCTTACG GACTTAGTTT TAACAAAGGA 
GAGGTGTTCC GGGTCGTGGA TACTTTATAC AATGGAAAGC TGGGCTCTTG GCTTGCCATT 
CGAATTGGCA AAAATCATAA GGAGGTAGAA CGAGGCATCA TCCCTAATAA GAACAGAGCT 
GAGCAGTTAG CCAGTGTACA GTACACACTT CCAAAGACAG CGGGTGGTGA TCGGGCAGAC 
TTCTGGAGGT TTCGAGGTCT TCGCAGCTCC AAGAGAAATC TTCGAAAAAG CAGAGAGGAC 
TTGTCAGCTC AGCCAGTTCA AACTAAGTTC CCAGCTTATG AAAGGGTTGT TCTTCGAGAA 
GCTGGATTCC TAAGACCTGT AACCATCTTT GGACCAATAG CTGATGTTGC CAGAGAAAAG 
TTGGCAAGAG AGGAACCAGA TATCTATCAG ATTGCAAAAA GTGAACCACG AGATGCTGGG 
ACTGACCATC GTAGCTCTGG CATCATTCGC CTTCATACAA TAAAGCAAAT CATAGATCAG 
GATAAACATG CGTTATTAGA TGTAACGCCA AATGCGGTTG ATCGTCTTAA TTATGCGCAG 
TGGTATCCAA TTGTTGTGTT CCTTAACCCT GACTCTAAGC AAGGTGTAAA AACAATGAGG 
ATGAGGTTAT GTCCGGAGTC TCGGAAAAGC GCCAGGAAGC TATATGAACG CTCTCATAAG 
CTTCGCAAAA ACAATCACCA CCTTTTCACA ACTACAATTA ACTTAAACTC AATGAATGAT 
GGTTGGTACG GTGCCCTGAA AGAAGCCATT CAGCAGCAAC AGAACCAGCT GGTGTGGGTC 
TCTGAGGGGA AGGCGGATGG TGCTACAAGT GATGACCTTG ATTTGCATGA TGATCGTCTG 
TCCTACCTGT CAGCCCCAGG TAGTGAGTAC TCAATGTATA GCACGGACAG TAGACACACT 
TCTGACTATG AAGACACAGA TACAGAAGGC GGGGCCTACA CTGATCAAGA ACTAGATGAA 
ACTCTTAATG ATGAGGTGGG GACTCCCCCG GAGTCTGCCA TTACACNGTC CTCTGAGCCT 
GTAAGAGAGG ATTCCTCTGG GATGCATCAT GAAAACCAGA CCTATCCTCC TTACTCACCA 
CAAGCGCAGC CACAAGCTAT TCATAGACTA GACTCCCCTG GACTTAAGCC AGCCTCTCAA 
CAGGTGTATA GAAAGGAGCC GTATCCTGAA GAAATGATGA GACAAAACCA TATTTTAAAA 
CAACCAGCTC TTGGTCACCC AGGGCAGAGG CCAGATAAAG AGCCAAATCT AGCCTATGAA 
CCCCAACTTC CATATANAGA AAAACAAGCC AGCAGAGACC TTGAACAGCC GTCATACAGG 
TATGAGGCCT CAAGCTACCC AGACCAGTTT TCTCGGAACT ATGACCATCG CCTACGGTTT 
GAAGATCGAA TCCCTACCTA TGAAGACCAG TGGTCATATT ATGATGACAA ACAGCCCTAC 
CAACCTCGGC CTTTTGAGAA TCAGCATCCC CGAGACCTGG ACTCCAGACA ACATCCNGAG 
GAGGCTTCAG AACGAGGTTA TNTCCAGCGT TTTGAAGAGC CAGCCCCTCT GTCATACGAC 
AGTAGAACAC GCTATGAGCA GCTGCCTCGA ACCTCTACTC TACGACATGA AGAGCAGCCA 
GCCCCTGCAT ATGAGGTGCA CAACAGGTAC AGGCCAGAGG CACAGCCCTA TTCTTCAACA 
GGTCCTAAGT CATCTGAGCC CAAGCAGTAC TTTGACCAGT ACCCGCGAAG TTATGAGCAA 
GTACCACCAC CAGGCTTTAC CTCCAAAACA GGCCATTACG AGCCTCTCCA TGGTGCTGCA 
GTTGTCCCTC CTCTGATACC TTCCTCTCAA CATAAGCCAG AAGTCCTGCC CTCGGCTACC 
AAACCACAGC CTCCACCCCC AACCCTAACT GAGGAGGAGG AGGATCCAGC AATGAAACCA 
CAGTCCGTGC TCANCAGAGT CAAAATGTTT GAAAACAAAA GATCTGCGTC TTTGGAGAAC 
AAGAAAGATG TGAATGACAC TGCCAGCTTT AAGCCTCCAG AAGTAGCATC TAAACCTCCA 
AGTGCTTCTC TTGCTGGCCC TAAACCTGTT CCTCAGAGTC AGTTTAGTGA GCANGACAAA 
ACACTCTACA GGCTCCCAGA GCCTCAGAAA CCTCAAGTGA AGCCACCCGA NGATATTGTT 
CGATCAAATC ATTACGACCC TGAAGAGGAT GAAGAATATT ACAGGAAACA GCTCTCTTAC 
TTTGANCGAA GAAGTTTTGA GAGCAAGCCT TCTGCACATC TTCCTGCTGG CCATCACTCA 
GAGCCTGCTA AGCCAGTCCA TTCTCAGAGC CAGCCCAACT TTTCTAGTTA CTCTTCAAAG 
GGAAAACCCG AAACTGATGC TGTGGATAGA TCATTCAGTG AGAAACGTTA TGATCCAGCC 
CAGGCCACGC CTCCTCCTCC TCCGTTGCCC TCACAGTACA GCCAGCCAGC TCCCCCTCTG 
TCCAGCTCTT CTCTCCACAT ACATTCCAAG GGCGCCCAGG GCGAAGGCAA TTCAGTATCA 
TTGGATTTTC AGAACTCATA TATGTCCAAA CCAGACCCAC CCCCATCTCA GAGTAAACCA 
GCAACTTTCA GACCACCAAC CCGAGAAGAC CCTCCTCAGA CTTTCTATCC CCAGAAANGT 
TTCCCAGACA AAGCTCCAGT TAANGGAGCT GAGCAGACTC AGAAAACCAT CACTCCAGTG 
TACAATCGAT TCACACCAAA GCCGTACACA AGTTCTGCCC GGCCGTTTGA ACGCAAATTT 
GAAAGTCCGA AGTTCAACCA TAATCTTCTG CCAAGTGAAA CTGTACATAA ACCTGAATTG 
TCTTCAAAAA CTCCCACTTC TCCAAAAACT CTTATGAAAG CTCATAGTTC AACACAGCCT 
CCAGAGTTTG ACAGTGGAGT TGAAACTTTC TCTGTTCACA CAGATNAGCC TAAATATCAA 
ATGAATAATA TCAGCACCAT GCCTAAAGCT GTCCCTGTGA GTCCTTCAGC TGTGGAAGAA 
GATGAAGATG AGGATGGTCA TACTGTAGTG GCTACAGCTC GTGGCATTTT TAACAGCAAT 
GGTGGTGTGT TGAGTTCCAT AGAAACCGGT GTTAGTATAA TTATCCCACA AGGAGCCATT 
CCTGAAGGAA TTGAACAAGA AATCTATTTC AAAGTCTGCA GAGACAATAG CATTCTCCCA 
CCTTTAGATA AAGAGAAAGG TGAAACTCTG CTAAGTCCCC TAGTGATGTG TGGGCCCCAT 
GGCCTCAAGT TCCTGAAGCC CGTGGAGCTG CGCTTGCCAC ACTGTGCGTC CATGACTCCT 
GACGGTTGGT CTTTTGCTCT AAAATCATCC GACTCCTCGT CGGGTGACCC TAAAACCTGG 
CAAAACAAGT GTCTTCCTGG AGATCCGAAT TACCTTGTTG GAGCCAACTG TGTTTCTGTC 
CTGATTGACC ACTTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021021825.1
CDS	38..5095
Translation	MSTRAAAAKSTAMEETAIWEQHTVMLHRAPGFGFGIAISGGRDNPHFQSGETSIVISDVLKGGPAEGQLQENDRVAMVNGVSMDNVEHAFAVQQLRKSGKNAKITMRRKKKVQIPVSHPDPEPVSDNEDDSYDEEVHDPRAGRSALANRRSEKSWARDRSASRERSLSPRSDRRSVASSQPAKPTKVALVKSRKNEEYGLRLASHIFVKEISQDSLAARDGNIQEGDVVLKINGTVTENMSLTDAKTLIERSKGKLKMVVQRDERATLLNVPDLSDSIHSANASERDDISEIQSLASDHSGRSHDRPPRRSQSRSPDQRSEPSDHSTQSPQQPSNGSLRSREQERMSKPGAISTPVKHADDHTPKAVEEVTVEKNEKQTPTLPEPKPVYAQVGQPDVDLPVSPSDGVLPNSTHEDGILRPSMKLVKFRKGDSVGLRLAGGNDVGIFVAGVLEDSPAAKEGLEEGDQILRVNNVDFTNIIREEAVLFLLDLPKGEEVTILAQKKKGVYRRIVESDVGDSFYIRTHFEYEKESPYGLSFNKGEVFRVVDTLYNGKLGSWLAIRIGKNHKEVERGIIPNKNRAEQLASVQYTLPKTAGGDRADFWRFRGLRSSKRNLRKSREDLSAQPVQTKFPAYERVVLREAGFLRPVTIFGPIADVAREKLAREEPDIYQIAKSEPRDAGTDHRSSGIIRLHTIKQIIDQDKHALLDVTPNAVDRLNYAQWYPIVVFLNPDSKQGVKTMRMRLCPESRKSARKLYERSHKLRKNNHHLFTTTINLNSMNDGWYGALKEAIQQQQNQLVWVSEGKADGATSDDLDLHDDRLSYLSAPGSEYSMYSTDSRHTSDYEDTDTEGGAYTDQELDETLNDEVGTPPESAITnullSSEPVREDSSGMHHENQTYPPYSPQAQPQAIHRLDSPGLKPASQQVYRKEPYPEEMMRQNHILKQPALGHPGQRPDKEPNLAYEPQLPYnullEKQASRDLEQPSYRYEASSYPDQFSRNYDHRLRFEDRIPTYEDQWSYYDDKQPYQPRPFENQHPRDLDSRQHnullEEASERGYnullQRFEEPAPLSYDSRTRYEQLPRTSTLRHEEQPAPAYEVHNRYRPEAQPYSSTGPKSSEPKQYFDQYPRSYEQVPPPGFTSKTGHYEPLHGAAVVPPLIPSSQHKPEVLPSATKPQPPPPTLTEEEEDPAMKPQSVLnullRVKMFENKRSASLENKKDVNDTASFKPPEVASKPPSASLAGPKPVPQSQFSEnullDKTLYRLPEPQKPQVKPPnullDIVRSNHYDPEEDEEYYRKQLSYFnullRRSFESKPSAHLPAGHHSEPAKPVHSQSQPNFSSYSSKGKPETDAVDRSFSEKRYDPAQATPPPPPLPSQYSQPAPPLSSSSLHIHSKGAQGEGNSVSLDFQNSYMSKPDPPPSQSKPATFRPPTREDPPQTFYPQKnullFPDKAPVnullGAEQTQKTITPVYNRFTPKPYTSSARPFERKFESPKFNHNLLPSETVHKPELSSKTPTSPKTLMKAHSSTQPPEFDSGVETFSVHTDnullPKYQMNNISTMPKAVPVSPSAVEEDEDEDGHTVVATARGIFNSNGGVLSSIETGVSIIIPQGAIPEGIEQEIYFKVCRDNSILPPLDKEKGETLLSPLVMCGPHGLKFLKPVELRLPHCASMTPDGWSFALKSSDSSSGDPKTWQNKCLPGDPNYLVGANCVSVLIDHF

Target ORF information:

RefSeq Version	XM_021166166.1
Organism	Mus caroli(Ryukyu mouse)
Definition	Mus caroli tight junction protein 1 (Tjp1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021166166.1

ORF Insert Sequence:

ATGTCCACCA GGGCCGCGGC CGCTAAGAGC ACAGCAATGG AGGAAACGGC TATATGGGAA 
CAGCACACAG TGATGCTTCA CAGGGCTCCT GGGTTTGGAT TTGGAATTGC AATCTCTGGT 
GGAAGAGATA ATCCTCATTT TCAGAGTGGG GAAACCTCCA TAGTGATTTC TGATGTGCTA 
AAAGGAGGGC CAGCTGAAGG ACAGCTACAG GAAAATGACC GAGTTGCAAT GGTTAATGGA 
GTTTCAATGG ATAACGTTGA ACATGCTTTT GCTGTTCAGC AGCTAAGGAA AAGTGGGAAA 
AACGCAAAAA TTACTATGCG AAGGAAGAAG AAAGTTCAGA TCCCTGTAAG TCACCCAGAT 
CCTGAGCCGG TGTCTGATAA TGAAGATGAT AGCTACGACG AAGAAGTACA TGATCCAAGA 
GCTGGCCGCA GTGCTTTAGC GAACAGAAGG AGCGAGAAGA GCTGGGCAAG GGATAGGAGT 
GCAAGCAGGG AGAGGAGCCT GTCCCCTCGC TCGGACAGGC GGTCGGTGGC CTCCAGTCAG 
CCCGCAAAAC CCACCAAGGT CGCACTGGTG AAGTCTCGGA AAAATGAAGA ATATGGTCTT 
CGATTGGCCA GCCATATATT TGTAAAGGAA ATTTCACAAG ATAGTTTGGC AGCAAGAGAT 
GGTAACATTC AAGAAGGGGA TGTTGTCTTG AAGATAAATG GTACTGTGAC AGAAAATATG 
TCATTGACAG ATGCAAAAAC ACTGATAGAA AGGTCTAAAG GCAAGTTAAA AATGGTAGTG 
CAAAGAGATG AGCGGGCTAC CTTACTGAAC GTCCCTGACC TTTCGGATAG TATCCATTCT 
GCTAATGCCT CGGAAAGAGA TGACATTTCA GAAATTCAGT CACTAGCATC AGACCATTCA 
GGTCGCTCGC ATGACAGGCC ACCCCGCCGC AGCCAGTCAC GATCTCCTGA CCAACGTTCA 
GAGCCCTCCG ATCATTCCAC ACAGTCTCCA CAGCAGCCCA GCAATGGCAG TCTCCGGAGC 
AGAGAGCAAG AGCGAATGTC TAAACCTGGG GCCATCTCAA CTCCTGTAAA ACATGCAGAT 
GATCATACAC CCAAAGCAGT GGAAGAAGTT ACAGTTGAGA AAAATGAGAA GCAGACACCC 
ACTCTTCCAG AACCGAAGCC TGTATATGCT CAAGTTGGAC AACCAGATGT GGATTTACCC 
GTCAGCCCTT CTGATGGTGT TCTGCCTAAT TCAACTCATG AAGATGGGAT ACTTAGGCCC 
AGCATGAAAC TGGTAAAATT CAGAAAAGGA GATAGTGTGG GTTTGCGACT AGCTGGTGGA 
AATGATGTCG GAATATTTGT AGCTGGCGTT CTAGAAGATA GCCCTGCAGC CAAAGAAGGC 
TTAGAGGAAG GTGATCAAAT TCTCAGGGTG AACAATGTAG ATTTCACAAA TATCATAAGA 
GAAGAGGCTG TCCTTTTCCT CCTTGACCTC CCTAAAGGTG AAGAAGTGAC CATCCTGGCT 
CAGAAGAAGA AGGGTGTTTA TCGCCGCATT GTAGAATCAG ATGTAGGAGA TTCATTCTAT 
ATTAGAACGC ATTTTGAGTA TGAAAAAGAA TCTCCTTACG GACTTAGTTT TAACAAAGGA 
GAGGTGTTCC GGGTCGTGGA TACTTTATAC AATGGAAAGC TGGGCTCTTG GCTTGCCATT 
CGAATTGGCA AAAATCATAA GGAGGTAGAA CGAGGCATCA TCCCTAATAA GAACAGAGCT 
GAGCAGTTAG CCAGTGTACA GTACACACTT CCAAAGACAG CGGGTGGTGA TCGGGCAGAC 
TTCTGGAGGT TTCGAGGTCT TCGCAGCTCC AAGAGAAATC TTCGAAAAAG CAGAGAGGAC 
TTGTCAGCTC AGCCAGTTCA AACTAAGTTC CCAGCTTATG AAAGGGTTGT TCTTCGAGAA 
GCTGGATTCC TAAGACCTGT AACCATCTTT GGACCAATAG CTGATGTTGC CAGAGAAAAG 
TTGGCAAGAG AGGAACCAGA TATCTATCAG ATTGCAAAAA GTGAACCACG AGATGCTGGG 
ACTGACCATC GTAGCTCTGG CATCATTCGC CTTCATACAA TAAAGCAAAT CATAGATCAG 
GATAAACATG CGTTATTAGA TGTAACGCCA AATGCGGTTG ATCGTCTTAA TTATGCGCAG 
TGGTATCCAA TTGTTGTGTT CCTTAACCCT GACTCTAAGC AAGGTGTAAA AACAATGAGG 
ATGAGGTTAT GTCCGGAGTC TCGGAAAAGC GCCAGGAAGC TATATGAACG CTCTCATAAG 
CTTCGCAAAA ACAATCACCA CCTTTTCACA ACTACAATTA ACTTAAACTC AATGAATGAT 
GGTTGGTACG GTGCCCTGAA AGAAGCCATT CAGCAGCAAC AGAACCAGCT GGTGTGGGTC 
TCTGAGGGGA AGGCGGATGG TGCTACAAGT GATGACCTTG ATTTGCATGA TGATCGTCTG 
TCCTACCTGT CAGCCCCAGG TAGTGAGTAC TCAATGTATA GCACGGACAG TAGACACACT 
TCTGACTATG AAGACACAGA TACAGAAGGC GGGGCCTACA CTGATCAAGA ACTAGATGAA 
ACTCTTAATG ATGAGGTGGG GACTCCCCCG GAGTCTGCCA TTACACNGTC CTCTGAGCCT 
GTAAGAGAGG ATTCCTCTGG GATGCATCAT GAAAACCAGA CCTATCCTCC TTACTCACCA 
CAAGCGCAGC CACAAGCTAT TCATAGACTA GACTCCCCTG GACTTAAGCC AGCCTCTCAA 
CAGGTGTATA GAAAGGAGCC GTATCCTGAA GAAATGATGA GACAAAACCA TATTTTAAAA 
CAACCAGCTC TTGGTCACCC AGGGCAGAGG CCAGATAAAG AGCCAAATCT AGCCTATGAA 
CCCCAACTTC CATATANAGA AAAACAAGCC AGCAGAGACC TTGAACAGCC GTCATACAGG 
TATGAGGCCT CAAGCTACCC AGACCAGTTT TCTCGGAACT ATGACCATCG CCTACGGTTT 
GAAGATCGAA TCCCTACCTA TGAAGACCAG TGGTCATATT ATGATGACAA ACAGCCCTAC 
CAACCTCGGC CTTTTGAGAA TCAGCATCCC CGAGACCTGG ACTCCAGACA ACATCCNGAG 
GAGGCTTCAG AACGAGGTTA TNTCCAGCGT TTTGAAGAGC CAGCCCCTCT GTCATACGAC 
AGTAGAACAC GCTATGAGCA GCTGCCTCGA ACCTCTACTC TACGACATGA AGAGCAGCCA 
GCCCCTGCAT ATGAGGTGCA CAACAGGTAC AGGCCAGAGG CACAGCCCTA TTCTTCAACA 
GGTCCTAAGT CATCTGAGCC CAAGCAGTAC TTTGACCAGT ACCCGCGAAG TTATGAGCAA 
GTACCACCAC CAGGCTTTAC CTCCAAAACA GGCCATTACG AGCCTCTCCA TGGTGCTGCA 
GTTGTCCCTC CTCTGATACC TTCCTCTCAA CATAAGCCAG AAGTCCTGCC CTCGGCTACC 
AAACCACAGC CTCCACCCCC AACCCTAACT GAGGAGGAGG AGGATCCAGC AATGAAACCA 
CAGTCCGTGC TCANCAGAGT CAAAATGTTT GAAAACAAAA GATCTGCGTC TTTGGAGAAC 
AAGAAAGATG TGAATGACAC TGCCAGCTTT AAGCCTCCAG AAGTAGCATC TAAACCTCCA 
AGTGCTTCTC TTGCTGGCCC TAAACCTGTT CCTCAGAGTC AGTTTAGTGA GCANGACAAA 
ACACTCTACA GGCTCCCAGA GCCTCAGAAA CCTCAAGTGA AGCCACCCGA NGATATTGTT 
CGATCAAATC ATTACGACCC TGAAGAGGAT GAAGAATATT ACAGGAAACA GCTCTCTTAC 
TTTGANCGAA GAAGTTTTGA GAGCAAGCCT TCTGCACATC TTCCTGCTGG CCATCACTCA 
GAGCCTGCTA AGCCAGTCCA TTCTCAGAGC CAGCCCAACT TTTCTAGTTA CTCTTCAAAG 
GGAAAACCCG AAACTGATGC TGTGGATAGA TCATTCAGTG AGAAACGTTA TGATCCAGCC 
CAGGCCACGC CTCCTCCTCC TCCGTTGCCC TCACAGTACA GCCAGCCAGC TCCCCCTCTG 
TCCAGCTCTT CTCTCCACAT ACATTCCAAG GGCGCCCAGG GCGAAGGCAA TTCAGTATCA 
TTGGATTTTC AGAACTCATA TATGTCCAAA CCAGACCCAC CCCCATCTCA GAGTAAACCA 
GCAACTTTCA GACCACCAAC CCGAGAAGAC CCTCCTCAGA CTTTCTATCC CCAGAAANGT 
TTCCCAGACA AAGCTCCAGT TAANGGAGCT GAGCAGACTC AGAAAACCAT CACTCCAGTG 
TACAATCGAT TCACACCAAA GCCGTACACA AGTTCTGCCC GGCCGTTTGA ACGCAAATTT 
GAAAGTCCGA AGTTCAACCA TAATCTTCTG CCAAGTGAAA CTGTACATAA ACCTGAATTG 
TCTTCAAAAA CTCCCACTTC TCCAAAAACT CTTATGAAAG CTCATAGTTC AACACAGCCT 
CCAGAGTTTG ACAGTGGAGT TGAAACTTTC TCTGTTCACA CAGATNAGCC TAAATATCAA 
ATGAATAATA TCAGCACCAT GCCTAAAGCT GTCCCTGTGA GTCCTTCAGC TGTGGAAGAA 
GATGAAGATG AGGATGGTCA TACTGTAGTG GCTACAGCTC GTGGCATTTT TAACAGCAAT 
GGTGGTGTGT TGAGTTCCAT AGAAACCGGT GTTAGTATAA TTATCCCACA AGGAGCCATT 
CCTGAAGGAA TTGAACAAGA AATCTATTTC AAAGTCTGCA GAGACAATAG CATTCTCCCA 
CCTTTAGATA AAGAGAAAGG TGAAACTCTG CTAAGTCCCC TAGTGATGTG TGGGCCCCAT 
GGCCTCAAGT TCCTGAAGCC CGTGGAGCTG CGCTTGCCAC ACTGTGCGTC CATGACTCCT 
GACGGTTGGT CTTTTGCTCT AAAATCATCC GACTCCTCGT CGGGTGACCC TAAAACCTGG 
CAAAACAAGT GTCTTCCTGG AGATCCGAAT TACCTTGTTG GAGCCAACTG TGTTTCTGTC 
CTGATTGACC ACTTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Tjp1 ( XM_021166166.2 ) cDNA ORF clone, Mus caroli(Ryukyu mouse) -> XP_021021825.1 Mus caroli tight junction protein 1 (Tjp1), transcript variant X9, mRNA.

Price & Availability↑

CloneID	OMv03980
Clone ID Related Accession (Same CDS sequence)	XM_021166166.1 , XM_021166166.2
Accession Version	XM_021166166.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5058bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-06-09
Organism	Mus caroli(Ryukyu mouse)
Product	tight junction protein ZO-1 isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_034576.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process On Jun 10, 2019 this sequence version replaced XM_021166166.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus caroli Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCACCA GGGCCGCGGC CGCTAAGAGC ACAGCAATGG AGGAAACGGC TATATGGGAA 
CAGCACACAG TGATGCTTCA CAGGGCTCCT GGGTTTGGAT TTGGAATTGC AATCTCTGGT 
GGAAGAGATA ATCCTCATTT TCAGAGTGGG GAAACCTCCA TAGTGATTTC TGATGTGCTA 
AAAGGAGGGC CAGCTGAAGG ACAGCTACAG GAAAATGACC GAGTTGCAAT GGTTAATGGA 
GTTTCAATGG ATAACGTTGA ACATGCTTTT GCTGTTCAGC AGCTAAGGAA AAGTGGGAAA 
AACGCAAAAA TTACTATGCG AAGGAAGAAG AAAGTTCAGA TCCCTGTAAG TCACCCAGAT 
CCTGAGCCGG TGTCTGATAA TGAAGATGAT AGCTACGACG AAGAAGTACA TGATCCAAGA 
GCTGGCCGCA GTGCTTTAGC GAACAGAAGG AGCGAGAAGA GCTGGGCAAG GGATAGGAGT 
GCAAGCAGGG AGAGGAGCCT GTCCCCTCGC TCGGACAGGC GGTCGGTGGC CTCCAGTCAG 
CCCGCAAAAC CCACCAAGGT CGCACTGGTG AAGTCTCGGA AAAATGAAGA ATATGGTCTT 
CGATTGGCCA GCCATATATT TGTAAAGGAA ATTTCACAAG ATAGTTTGGC AGCAAGAGAT 
GGTAACATTC AAGAAGGGGA TGTTGTCTTG AAGATAAATG GTACTGTGAC AGAAAATATG 
TCATTGACAG ATGCAAAAAC ACTGATAGAA AGGTCTAAAG GCAAGTTAAA AATGGTAGTG 
CAAAGAGATG AGCGGGCTAC CTTACTGAAC GTCCCTGACC TTTCGGATAG TATCCATTCT 
GCTAATGCCT CGGAAAGAGA TGACATTTCA GAAATTCAGT CACTAGCATC AGACCATTCA 
GGTCGCTCGC ATGACAGGCC ACCCCGCCGC AGCCAGTCAC GATCTCCTGA CCAACGTTCA 
GAGCCCTCCG ATCATTCCAC ACAGTCTCCA CAGCAGCCCA GCAATGGCAG TCTCCGGAGC 
AGAGAGCAAG AGCGAATGTC TAAACCTGGG GCCATCTCAA CTCCTGTAAA ACATGCAGAT 
GATCATACAC CCAAAGCAGT GGAAGAAGTT ACAGTTGAGA AAAATGAGAA GCAGACACCC 
ACTCTTCCAG AACCGAAGCC TGTATATGCT CAAGTTGGAC AACCAGATGT GGATTTACCC 
GTCAGCCCTT CTGATGGTGT TCTGCCTAAT TCAACTCATG AAGATGGGAT ACTTAGGCCC 
AGCATGAAAC TGGTAAAATT CAGAAAAGGA GATAGTGTGG GTTTGCGACT AGCTGGTGGA 
AATGATGTCG GAATATTTGT AGCTGGCGTT CTAGAAGATA GCCCTGCAGC CAAAGAAGGC 
TTAGAGGAAG GTGATCAAAT TCTCAGGGTG AACAATGTAG ATTTCACAAA TATCATAAGA 
GAAGAGGCTG TCCTTTTCCT CCTTGACCTC CCTAAAGGTG AAGAAGTGAC CATCCTGGCT 
CAGAAGAAGA AGGGTGTTTA TCGCCGCATT GTAGAATCAG ATGTAGGAGA TTCATTCTAT 
ATTAGAACGC ATTTTGAGTA TGAAAAAGAA TCTCCTTACG GACTTAGTTT TAACAAAGGA 
GAGGTGTTCC GGGTCGTGGA TACTTTATAC AATGGAAAGC TGGGCTCTTG GCTTGCCATT 
CGAATTGGCA AAAATCATAA GGAGGTAGAA CGAGGCATCA TCCCTAATAA GAACAGAGCT 
GAGCAGTTAG CCAGTGTACA GTACACACTT CCAAAGACAG CGGGTGGTGA TCGGGCAGAC 
TTCTGGAGGT TTCGAGGTCT TCGCAGCTCC AAGAGAAATC TTCGAAAAAG CAGAGAGGAC 
TTGTCAGCTC AGCCAGTTCA AACTAAGTTC CCAGCTTATG AAAGGGTTGT TCTTCGAGAA 
GCTGGATTCC TAAGACCTGT AACCATCTTT GGACCAATAG CTGATGTTGC CAGAGAAAAG 
TTGGCAAGAG AGGAACCAGA TATCTATCAG ATTGCAAAAA GTGAACCACG AGATGCTGGG 
ACTGACCATC GTAGCTCTGG CATCATTCGC CTTCATACAA TAAAGCAAAT CATAGATCAG 
GATAAACATG CGTTATTAGA TGTAACGCCA AATGCGGTTG ATCGTCTTAA TTATGCGCAG 
TGGTATCCAA TTGTTGTGTT CCTTAACCCT GACTCTAAGC AAGGTGTAAA AACAATGAGG 
ATGAGGTTAT GTCCGGAGTC TCGGAAAAGC GCCAGGAAGC TATATGAACG CTCTCATAAG 
CTTCGCAAAA ACAATCACCA CCTTTTCACA ACTACAATTA ACTTAAACTC AATGAATGAT 
GGTTGGTACG GTGCCCTGAA AGAAGCCATT CAGCAGCAAC AGAACCAGCT GGTGTGGGTC 
TCTGAGGGGA AGGCGGATGG TGCTACAAGT GATGACCTTG ATTTGCATGA TGATCGTCTG 
TCCTACCTGT CAGCCCCAGG TAGTGAGTAC TCAATGTATA GCACGGACAG TAGACACACT 
TCTGACTATG AAGACACAGA TACAGAAGGC GGGGCCTACA CTGATCAAGA ACTAGATGAA 
ACTCTTAATG ATGAGGTGGG GACTCCCCCG GAGTCTGCCA TTACACNGTC CTCTGAGCCT 
GTAAGAGAGG ATTCCTCTGG GATGCATCAT GAAAACCAGA CCTATCCTCC TTACTCACCA 
CAAGCGCAGC CACAAGCTAT TCATAGACTA GACTCCCCTG GACTTAAGCC AGCCTCTCAA 
CAGGTGTATA GAAAGGAGCC GTATCCTGAA GAAATGATGA GACAAAACCA TATTTTAAAA 
CAACCAGCTC TTGGTCACCC AGGGCAGAGG CCAGATAAAG AGCCAAATCT AGCCTATGAA 
CCCCAACTTC CATATANAGA AAAACAAGCC AGCAGAGACC TTGAACAGCC GTCATACAGG 
TATGAGGCCT CAAGCTACCC AGACCAGTTT TCTCGGAACT ATGACCATCG CCTACGGTTT 
GAAGATCGAA TCCCTACCTA TGAAGACCAG TGGTCATATT ATGATGACAA ACAGCCCTAC 
CAACCTCGGC CTTTTGAGAA TCAGCATCCC CGAGACCTGG ACTCCAGACA ACATCCNGAG 
GAGGCTTCAG AACGAGGTTA TNTCCAGCGT TTTGAAGAGC CAGCCCCTCT GTCATACGAC 
AGTAGAACAC GCTATGAGCA GCTGCCTCGA ACCTCTACTC TACGACATGA AGAGCAGCCA 
GCCCCTGCAT ATGAGGTGCA CAACAGGTAC AGGCCAGAGG CACAGCCCTA TTCTTCAACA 
GGTCCTAAGT CATCTGAGCC CAAGCAGTAC TTTGACCAGT ACCCGCGAAG TTATGAGCAA 
GTACCACCAC CAGGCTTTAC CTCCAAAACA GGCCATTACG AGCCTCTCCA TGGTGCTGCA 
GTTGTCCCTC CTCTGATACC TTCCTCTCAA CATAAGCCAG AAGTCCTGCC CTCGGCTACC 
AAACCACAGC CTCCACCCCC AACCCTAACT GAGGAGGAGG AGGATCCAGC AATGAAACCA 
CAGTCCGTGC TCANCAGAGT CAAAATGTTT GAAAACAAAA GATCTGCGTC TTTGGAGAAC 
AAGAAAGATG TGAATGACAC TGCCAGCTTT AAGCCTCCAG AAGTAGCATC TAAACCTCCA 
AGTGCTTCTC TTGCTGGCCC TAAACCTGTT CCTCAGAGTC AGTTTAGTGA GCANGACAAA 
ACACTCTACA GGCTCCCAGA GCCTCAGAAA CCTCAAGTGA AGCCACCCGA NGATATTGTT 
CGATCAAATC ATTACGACCC TGAAGAGGAT GAAGAATATT ACAGGAAACA GCTCTCTTAC 
TTTGANCGAA GAAGTTTTGA GAGCAAGCCT TCTGCACATC TTCCTGCTGG CCATCACTCA 
GAGCCTGCTA AGCCAGTCCA TTCTCAGAGC CAGCCCAACT TTTCTAGTTA CTCTTCAAAG 
GGAAAACCCG AAACTGATGC TGTGGATAGA TCATTCAGTG AGAAACGTTA TGATCCAGCC 
CAGGCCACGC CTCCTCCTCC TCCGTTGCCC TCACAGTACA GCCAGCCAGC TCCCCCTCTG 
TCCAGCTCTT CTCTCCACAT ACATTCCAAG GGCGCCCAGG GCGAAGGCAA TTCAGTATCA 
TTGGATTTTC AGAACTCATA TATGTCCAAA CCAGACCCAC CCCCATCTCA GAGTAAACCA 
GCAACTTTCA GACCACCAAC CCGAGAAGAC CCTCCTCAGA CTTTCTATCC CCAGAAANGT 
TTCCCAGACA AAGCTCCAGT TAANGGAGCT GAGCAGACTC AGAAAACCAT CACTCCAGTG 
TACAATCGAT TCACACCAAA GCCGTACACA AGTTCTGCCC GGCCGTTTGA ACGCAAATTT 
GAAAGTCCGA AGTTCAACCA TAATCTTCTG CCAAGTGAAA CTGTACATAA ACCTGAATTG 
TCTTCAAAAA CTCCCACTTC TCCAAAAACT CTTATGAAAG CTCATAGTTC AACACAGCCT 
CCAGAGTTTG ACAGTGGAGT TGAAACTTTC TCTGTTCACA CAGATNAGCC TAAATATCAA 
ATGAATAATA TCAGCACCAT GCCTAAAGCT GTCCCTGTGA GTCCTTCAGC TGTGGAAGAA 
GATGAAGATG AGGATGGTCA TACTGTAGTG GCTACAGCTC GTGGCATTTT TAACAGCAAT 
GGTGGTGTGT TGAGTTCCAT AGAAACCGGT GTTAGTATAA TTATCCCACA AGGAGCCATT 
CCTGAAGGAA TTGAACAAGA AATCTATTTC AAAGTCTGCA GAGACAATAG CATTCTCCCA 
CCTTTAGATA AAGAGAAAGG TGAAACTCTG CTAAGTCCCC TAGTGATGTG TGGGCCCCAT 
GGCCTCAAGT TCCTGAAGCC CGTGGAGCTG CGCTTGCCAC ACTGTGCGTC CATGACTCCT 
GACGGTTGGT CTTTTGCTCT AAAATCATCC GACTCCTCGT CGGGTGACCC TAAAACCTGG 
CAAAACAAGT GTCTTCCTGG AGATCCGAAT TACCTTGTTG GAGCCAACTG TGTTTCTGTC 
CTGATTGACC ACTTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021021825.1
CDS	65..5122
Translation	MSTRAAAAKSTAMEETAIWEQHTVMLHRAPGFGFGIAISGGRDNPHFQSGETSIVISDVLKGGPAEGQLQENDRVAMVNGVSMDNVEHAFAVQQLRKSGKNAKITMRRKKKVQIPVSHPDPEPVSDNEDDSYDEEVHDPRAGRSALANRRSEKSWARDRSASRERSLSPRSDRRSVASSQPAKPTKVALVKSRKNEEYGLRLASHIFVKEISQDSLAARDGNIQEGDVVLKINGTVTENMSLTDAKTLIERSKGKLKMVVQRDERATLLNVPDLSDSIHSANASERDDISEIQSLASDHSGRSHDRPPRRSQSRSPDQRSEPSDHSTQSPQQPSNGSLRSREQERMSKPGAISTPVKHADDHTPKAVEEVTVEKNEKQTPTLPEPKPVYAQVGQPDVDLPVSPSDGVLPNSTHEDGILRPSMKLVKFRKGDSVGLRLAGGNDVGIFVAGVLEDSPAAKEGLEEGDQILRVNNVDFTNIIREEAVLFLLDLPKGEEVTILAQKKKGVYRRIVESDVGDSFYIRTHFEYEKESPYGLSFNKGEVFRVVDTLYNGKLGSWLAIRIGKNHKEVERGIIPNKNRAEQLASVQYTLPKTAGGDRADFWRFRGLRSSKRNLRKSREDLSAQPVQTKFPAYERVVLREAGFLRPVTIFGPIADVAREKLAREEPDIYQIAKSEPRDAGTDHRSSGIIRLHTIKQIIDQDKHALLDVTPNAVDRLNYAQWYPIVVFLNPDSKQGVKTMRMRLCPESRKSARKLYERSHKLRKNNHHLFTTTINLNSMNDGWYGALKEAIQQQQNQLVWVSEGKADGATSDDLDLHDDRLSYLSAPGSEYSMYSTDSRHTSDYEDTDTEGGAYTDQELDETLNDEVGTPPESAITnullSSEPVREDSSGMHHENQTYPPYSPQAQPQAIHRLDSPGLKPASQQVYRKEPYPEEMMRQNHILKQPALGHPGQRPDKEPNLAYEPQLPYnullEKQASRDLEQPSYRYEASSYPDQFSRNYDHRLRFEDRIPTYEDQWSYYDDKQPYQPRPFENQHPRDLDSRQHnullEEASERGYnullQRFEEPAPLSYDSRTRYEQLPRTSTLRHEEQPAPAYEVHNRYRPEAQPYSSTGPKSSEPKQYFDQYPRSYEQVPPPGFTSKTGHYEPLHGAAVVPPLIPSSQHKPEVLPSATKPQPPPPTLTEEEEDPAMKPQSVLnullRVKMFENKRSASLENKKDVNDTASFKPPEVASKPPSASLAGPKPVPQSQFSEnullDKTLYRLPEPQKPQVKPPnullDIVRSNHYDPEEDEEYYRKQLSYFnullRRSFESKPSAHLPAGHHSEPAKPVHSQSQPNFSSYSSKGKPETDAVDRSFSEKRYDPAQATPPPPPLPSQYSQPAPPLSSSSLHIHSKGAQGEGNSVSLDFQNSYMSKPDPPPSQSKPATFRPPTREDPPQTFYPQKnullFPDKAPVnullGAEQTQKTITPVYNRFTPKPYTSSARPFERKFESPKFNHNLLPSETVHKPELSSKTPTSPKTLMKAHSSTQPPEFDSGVETFSVHTDnullPKYQMNNISTMPKAVPVSPSAVEEDEDEDGHTVVATARGIFNSNGGVLSSIETGVSIIIPQGAIPEGIEQEIYFKVCRDNSILPPLDKEKGETLLSPLVMCGPHGLKFLKPVELRLPHCASMTPDGWSFALKSSDSSSGDPKTWQNKCLPGDPNYLVGANCVSVLIDHF

Target ORF information:

RefSeq Version	XM_021166166.2
Organism	Mus caroli(Ryukyu mouse)
Definition	Mus caroli tight junction protein 1 (Tjp1), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021166166.2

ORF Insert Sequence:

ATGTCCACCA GGGCCGCGGC CGCTAAGAGC ACAGCAATGG AGGAAACGGC TATATGGGAA 
CAGCACACAG TGATGCTTCA CAGGGCTCCT GGGTTTGGAT TTGGAATTGC AATCTCTGGT 
GGAAGAGATA ATCCTCATTT TCAGAGTGGG GAAACCTCCA TAGTGATTTC TGATGTGCTA 
AAAGGAGGGC CAGCTGAAGG ACAGCTACAG GAAAATGACC GAGTTGCAAT GGTTAATGGA 
GTTTCAATGG ATAACGTTGA ACATGCTTTT GCTGTTCAGC AGCTAAGGAA AAGTGGGAAA 
AACGCAAAAA TTACTATGCG AAGGAAGAAG AAAGTTCAGA TCCCTGTAAG TCACCCAGAT 
CCTGAGCCGG TGTCTGATAA TGAAGATGAT AGCTACGACG AAGAAGTACA TGATCCAAGA 
GCTGGCCGCA GTGCTTTAGC GAACAGAAGG AGCGAGAAGA GCTGGGCAAG GGATAGGAGT 
GCAAGCAGGG AGAGGAGCCT GTCCCCTCGC TCGGACAGGC GGTCGGTGGC CTCCAGTCAG 
CCCGCAAAAC CCACCAAGGT CGCACTGGTG AAGTCTCGGA AAAATGAAGA ATATGGTCTT 
CGATTGGCCA GCCATATATT TGTAAAGGAA ATTTCACAAG ATAGTTTGGC AGCAAGAGAT 
GGTAACATTC AAGAAGGGGA TGTTGTCTTG AAGATAAATG GTACTGTGAC AGAAAATATG 
TCATTGACAG ATGCAAAAAC ACTGATAGAA AGGTCTAAAG GCAAGTTAAA AATGGTAGTG 
CAAAGAGATG AGCGGGCTAC CTTACTGAAC GTCCCTGACC TTTCGGATAG TATCCATTCT 
GCTAATGCCT CGGAAAGAGA TGACATTTCA GAAATTCAGT CACTAGCATC AGACCATTCA 
GGTCGCTCGC ATGACAGGCC ACCCCGCCGC AGCCAGTCAC GATCTCCTGA CCAACGTTCA 
GAGCCCTCCG ATCATTCCAC ACAGTCTCCA CAGCAGCCCA GCAATGGCAG TCTCCGGAGC 
AGAGAGCAAG AGCGAATGTC TAAACCTGGG GCCATCTCAA CTCCTGTAAA ACATGCAGAT 
GATCATACAC CCAAAGCAGT GGAAGAAGTT ACAGTTGAGA AAAATGAGAA GCAGACACCC 
ACTCTTCCAG AACCGAAGCC TGTATATGCT CAAGTTGGAC AACCAGATGT GGATTTACCC 
GTCAGCCCTT CTGATGGTGT TCTGCCTAAT TCAACTCATG AAGATGGGAT ACTTAGGCCC 
AGCATGAAAC TGGTAAAATT CAGAAAAGGA GATAGTGTGG GTTTGCGACT AGCTGGTGGA 
AATGATGTCG GAATATTTGT AGCTGGCGTT CTAGAAGATA GCCCTGCAGC CAAAGAAGGC 
TTAGAGGAAG GTGATCAAAT TCTCAGGGTG AACAATGTAG ATTTCACAAA TATCATAAGA 
GAAGAGGCTG TCCTTTTCCT CCTTGACCTC CCTAAAGGTG AAGAAGTGAC CATCCTGGCT 
CAGAAGAAGA AGGGTGTTTA TCGCCGCATT GTAGAATCAG ATGTAGGAGA TTCATTCTAT 
ATTAGAACGC ATTTTGAGTA TGAAAAAGAA TCTCCTTACG GACTTAGTTT TAACAAAGGA 
GAGGTGTTCC GGGTCGTGGA TACTTTATAC AATGGAAAGC TGGGCTCTTG GCTTGCCATT 
CGAATTGGCA AAAATCATAA GGAGGTAGAA CGAGGCATCA TCCCTAATAA GAACAGAGCT 
GAGCAGTTAG CCAGTGTACA GTACACACTT CCAAAGACAG CGGGTGGTGA TCGGGCAGAC 
TTCTGGAGGT TTCGAGGTCT TCGCAGCTCC AAGAGAAATC TTCGAAAAAG CAGAGAGGAC 
TTGTCAGCTC AGCCAGTTCA AACTAAGTTC CCAGCTTATG AAAGGGTTGT TCTTCGAGAA 
GCTGGATTCC TAAGACCTGT AACCATCTTT GGACCAATAG CTGATGTTGC CAGAGAAAAG 
TTGGCAAGAG AGGAACCAGA TATCTATCAG ATTGCAAAAA GTGAACCACG AGATGCTGGG 
ACTGACCATC GTAGCTCTGG CATCATTCGC CTTCATACAA TAAAGCAAAT CATAGATCAG 
GATAAACATG CGTTATTAGA TGTAACGCCA AATGCGGTTG ATCGTCTTAA TTATGCGCAG 
TGGTATCCAA TTGTTGTGTT CCTTAACCCT GACTCTAAGC AAGGTGTAAA AACAATGAGG 
ATGAGGTTAT GTCCGGAGTC TCGGAAAAGC GCCAGGAAGC TATATGAACG CTCTCATAAG 
CTTCGCAAAA ACAATCACCA CCTTTTCACA ACTACAATTA ACTTAAACTC AATGAATGAT 
GGTTGGTACG GTGCCCTGAA AGAAGCCATT CAGCAGCAAC AGAACCAGCT GGTGTGGGTC 
TCTGAGGGGA AGGCGGATGG TGCTACAAGT GATGACCTTG ATTTGCATGA TGATCGTCTG 
TCCTACCTGT CAGCCCCAGG TAGTGAGTAC TCAATGTATA GCACGGACAG TAGACACACT 
TCTGACTATG AAGACACAGA TACAGAAGGC GGGGCCTACA CTGATCAAGA ACTAGATGAA 
ACTCTTAATG ATGAGGTGGG GACTCCCCCG GAGTCTGCCA TTACACNGTC CTCTGAGCCT 
GTAAGAGAGG ATTCCTCTGG GATGCATCAT GAAAACCAGA CCTATCCTCC TTACTCACCA 
CAAGCGCAGC CACAAGCTAT TCATAGACTA GACTCCCCTG GACTTAAGCC AGCCTCTCAA 
CAGGTGTATA GAAAGGAGCC GTATCCTGAA GAAATGATGA GACAAAACCA TATTTTAAAA 
CAACCAGCTC TTGGTCACCC AGGGCAGAGG CCAGATAAAG AGCCAAATCT AGCCTATGAA 
CCCCAACTTC CATATANAGA AAAACAAGCC AGCAGAGACC TTGAACAGCC GTCATACAGG 
TATGAGGCCT CAAGCTACCC AGACCAGTTT TCTCGGAACT ATGACCATCG CCTACGGTTT 
GAAGATCGAA TCCCTACCTA TGAAGACCAG TGGTCATATT ATGATGACAA ACAGCCCTAC 
CAACCTCGGC CTTTTGAGAA TCAGCATCCC CGAGACCTGG ACTCCAGACA ACATCCNGAG 
GAGGCTTCAG AACGAGGTTA TNTCCAGCGT TTTGAAGAGC CAGCCCCTCT GTCATACGAC 
AGTAGAACAC GCTATGAGCA GCTGCCTCGA ACCTCTACTC TACGACATGA AGAGCAGCCA 
GCCCCTGCAT ATGAGGTGCA CAACAGGTAC AGGCCAGAGG CACAGCCCTA TTCTTCAACA 
GGTCCTAAGT CATCTGAGCC CAAGCAGTAC TTTGACCAGT ACCCGCGAAG TTATGAGCAA 
GTACCACCAC CAGGCTTTAC CTCCAAAACA GGCCATTACG AGCCTCTCCA TGGTGCTGCA 
GTTGTCCCTC CTCTGATACC TTCCTCTCAA CATAAGCCAG AAGTCCTGCC CTCGGCTACC 
AAACCACAGC CTCCACCCCC AACCCTAACT GAGGAGGAGG AGGATCCAGC AATGAAACCA 
CAGTCCGTGC TCANCAGAGT CAAAATGTTT GAAAACAAAA GATCTGCGTC TTTGGAGAAC 
AAGAAAGATG TGAATGACAC TGCCAGCTTT AAGCCTCCAG AAGTAGCATC TAAACCTCCA 
AGTGCTTCTC TTGCTGGCCC TAAACCTGTT CCTCAGAGTC AGTTTAGTGA GCANGACAAA 
ACACTCTACA GGCTCCCAGA GCCTCAGAAA CCTCAAGTGA AGCCACCCGA NGATATTGTT 
CGATCAAATC ATTACGACCC TGAAGAGGAT GAAGAATATT ACAGGAAACA GCTCTCTTAC 
TTTGANCGAA GAAGTTTTGA GAGCAAGCCT TCTGCACATC TTCCTGCTGG CCATCACTCA 
GAGCCTGCTA AGCCAGTCCA TTCTCAGAGC CAGCCCAACT TTTCTAGTTA CTCTTCAAAG 
GGAAAACCCG AAACTGATGC TGTGGATAGA TCATTCAGTG AGAAACGTTA TGATCCAGCC 
CAGGCCACGC CTCCTCCTCC TCCGTTGCCC TCACAGTACA GCCAGCCAGC TCCCCCTCTG 
TCCAGCTCTT CTCTCCACAT ACATTCCAAG GGCGCCCAGG GCGAAGGCAA TTCAGTATCA 
TTGGATTTTC AGAACTCATA TATGTCCAAA CCAGACCCAC CCCCATCTCA GAGTAAACCA 
GCAACTTTCA GACCACCAAC CCGAGAAGAC CCTCCTCAGA CTTTCTATCC CCAGAAANGT 
TTCCCAGACA AAGCTCCAGT TAANGGAGCT GAGCAGACTC AGAAAACCAT CACTCCAGTG 
TACAATCGAT TCACACCAAA GCCGTACACA AGTTCTGCCC GGCCGTTTGA ACGCAAATTT 
GAAAGTCCGA AGTTCAACCA TAATCTTCTG CCAAGTGAAA CTGTACATAA ACCTGAATTG 
TCTTCAAAAA CTCCCACTTC TCCAAAAACT CTTATGAAAG CTCATAGTTC AACACAGCCT 
CCAGAGTTTG ACAGTGGAGT TGAAACTTTC TCTGTTCACA CAGATNAGCC TAAATATCAA 
ATGAATAATA TCAGCACCAT GCCTAAAGCT GTCCCTGTGA GTCCTTCAGC TGTGGAAGAA 
GATGAAGATG AGGATGGTCA TACTGTAGTG GCTACAGCTC GTGGCATTTT TAACAGCAAT 
GGTGGTGTGT TGAGTTCCAT AGAAACCGGT GTTAGTATAA TTATCCCACA AGGAGCCATT 
CCTGAAGGAA TTGAACAAGA AATCTATTTC AAAGTCTGCA GAGACAATAG CATTCTCCCA 
CCTTTAGATA AAGAGAAAGG TGAAACTCTG CTAAGTCCCC TAGTGATGTG TGGGCCCCAT 
GGCCTCAAGT TCCTGAAGCC CGTGGAGCTG CGCTTGCCAC ACTGTGCGTC CATGACTCCT 
GACGGTTGGT CTTTTGCTCT AAAATCATCC GACTCCTCGT CGGGTGACCC TAAAACCTGG 
CAAAACAAGT GTCTTCCTGG AGATCCGAAT TACCTTGTTG GAGCCAACTG TGTTTCTGTC 
CTGATTGACC ACTTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Tjp1 ( XM_021166167.1 ) cDNA ORF clone, Mus caroli(Ryukyu mouse) -> XP_021021826.1 Mus caroli tight junction protein 1 (Tjp1), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OMv03981
Clone ID Related Accession (Same CDS sequence)	XM_021166167.1 , XM_021166167.2
Accession Version	XM_021166167.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4998bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-05-20
Organism	Mus caroli(Ryukyu mouse)
Product	tight junction protein ZO-1 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_034576.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus caroli Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCACCA GGGCCGCGGC CGCTAAGAGC ACAGCAATGG AGGAAACGGC TATATGGGAA 
CAGCACACAG TGATGCTTCA CAGGGCTCCT GGGTTTGGAT TTGGAATTGC AATCTCTGGT 
GGAAGAGATA ATCCTCATTT TCAGAGTGGG GAAACCTCCA TAGTGATTTC TGATGTGCTA 
AAAGGAGGGC CAGCTGAAGG ACAGCTACAG GAAAATGACC GAGTTGCAAT GGTTAATGGA 
GTTTCAATGG ATAACGTTGA ACATGCTTTT GCTGTTCAGC AGCTAAGGAA AAGTGGGAAA 
AACGCAAAAA TTACTATGCG AAGGAAGAAG AAAGTTCAGA TCCCTGTAAG TCACCCAGAT 
CCTGAGCCGG TGTCTGATAA TGAAGATGAT AGCTACGACG AAGAAGTACA TGATCCAAGA 
GCTGGCCGCA GTGCTTTAGC GAACAGAAGG AGCGAGAAGA GCTGGGCAAG GGATAGGAGT 
GCAAGCAGGG AGAGGAGCCT GTCCCCTCGC TCGGACAGGC GGTCGGTGGC CTCCAGTCAG 
CCCGCAAAAC CCACCAAGGT CGCACTGGTG AAGTCTCGGA AAAATGAAGA ATATGGTCTT 
CGATTGGCCA GCCATATATT TGTAAAGGAA ATTTCACAAG ATAGTTTGGC AGCAAGAGAT 
GGTAACATTC AAGAAGGGGA TGTTGTCTTG AAGATAAATG GTACTGTGAC AGAAAATATG 
TCATTGACAG ATGCAAAAAC ACTGATAGAA AGGTCTAAAG GCAAGTTAAA AATGGTAGTG 
CAAAGAGATG AGCGGGCTAC CTTACTGAAC GTCCCTGACC TTTCGGATAG TATCCATTCT 
GCTAATGCCT CGGAAAGAGA TGACATTTCA GAAATTCAGT CACTAGCATC AGACCATTCA 
GGTCGCTCGC ATGACAGGCC ACCCCGCCGC AGCCAGTCAC GATCTCCTGA CCAACGTTCA 
GAGCCCTCCG ATCATTCCAC ACAGTCTCCA CAGCAGCCCA GCAATGGCAG TCTCCGGAGC 
AGAGAGCAAG AGCGAATGTC TAAACCTGGG GCCATCTCAA CTCCTGTAAA ACATGCAGAT 
GATCATACAC CCAAAGCAGT GGAAGAAGTT ACAGTTGAGA AAAATGAGAA GCAGACACCC 
ACTCTTCCAG AACCGAAGCC TGTATATGCT CAAGTTGGAC AACCAGATGT GGATTTACCC 
GTCAGCCCTT CTGATGGTGT TCTGCCTAAT TCAACTCATG AAGATGGGAT ACTTAGGCCC 
AGCATGAAAC TGGTAAAATT CAGAAAAGGA GATAGTGTGG GTTTGCGACT AGCTGGTGGA 
AATGATGTCG GAATATTTGT AGCTGGCGTT CTAGAAGATA GCCCTGCAGC CAAAGAAGGC 
TTAGAGGAAG GTGATCAAAT TCTCAGGGTG AACAATGTAG ATTTCACAAA TATCATAAGA 
GAAGAGGCTG TCCTTTTCCT CCTTGACCTC CCTAAAGGTG AAGAAGTGAC CATCCTGGCT 
CAGAAGAAGA AGGGTGTTTA TCGCCGCATT GTAGAATCAG ATGTAGGAGA TTCATTCTAT 
ATTAGAACGC ATTTTGAGTA TGAAAAAGAA TCTCCTTACG GACTTAGTTT TAACAAAGGA 
GAGGTGTTCC GGGTCGTGGA TACTTTATAC AATGGAAAGC TGGGCTCTTG GCTTGCCATT 
CGAATTGGCA AAAATCATAA GGAGGTAGAA CGAGGCATCA TCCCTAATAA GAACAGAGCT 
GAGCAGTTAG CCAGTGTACA GTACACACTT CCAAAGACAG CGGGTGGTGA TCGGGCAGAC 
TTCTGGAGGT TTCGAGGTCT TCGCAGCTCC AAGAGAAATC TTCGAAAAAG CAGAGAGGAC 
TTGTCAGCTC AGCCAGTTCA AACTAAGTTC CCAGCTTATG AAAGGGTTGT TCTTCGAGAA 
GCTGGATTCC TAAGACCTGT AACCATCTTT GGACCAATAG CTGATGTTGC CAGAGAAAAG 
TTGGCAAGAG AGGAACCAGA TATCTATCAG ATTGCAAAAA GTGAACCACG AGATGCTGGG 
ACTGACCATC GTAGCTCTGG CATCATTCGC CTTCATACAA TAAAGCAAAT CATAGATCAG 
GATAAACATG CGTTATTAGA TGTAACGCCA AATGCGGTTG ATCGTCTTAA TTATGCGCAG 
TGGTATCCAA TTGTTGTGTT CCTTAACCCT GACTCTAAGC AAGGTGTAAA AACAATGAGG 
ATGAGGTTAT GTCCGGAGTC TCGGAAAAGC GCCAGGAAGC TATATGAACG CTCTCATAAG 
CTTCGCAAAA ACAATCACCA CCTTTTCACA ACTACAATTA ACTTAAACTC AATGAATGAT 
GGTTGGTACG GTGCCCTGAA AGAAGCCATT CAGCAGCAAC AGAACCAGCT GGTGTGGGTC 
TCTGAGGGGA AGGCGGATGG TGCTACAAGT GATGACCTTG ATTTGCATGA TGATCGTCTG 
TCCTACCTGT CAGCCCCAGG TAGTGAGTAC TCAATGTATA GCACGGACAG TAGACACACT 
TCTGACTATG AAGACACAGA TACAGAAGGC GGGGCCTACA CTGATCAAGA ACTAGATGAA 
ACTCTTAATG ATGAGGTGGG GACTCCCCCG GAGTCTGCCA TTACACNGTC CTCTGAGCCT 
GTAAGAGAGG ATTCCTCTGG GATGCATCAT GAAAACCAGA CCTATCCTCC TTACTCACCA 
CAAGCGCAGC CACAAGCTAT TCATAGACTA GACTCCCCTG GACTTAAGCC AGCCTCTCAA 
CAGGTGTATA GAAAGGAGCC GTATCCTGAA GAAATGATGA GACAAAACCA TATTTTAAAA 
CAACCAGCTC TTGGTCACCC AGGGCAGAGG CCAGATAAAG AGCCAAATCT AGCCTATGAA 
CCCCAACTTC CATATANAGA AAAACAAGCC AGCAGAGACC TTGAACAGCC GTCATACAGG 
TATGAGGCCT CAAGCTACCC AGACCAGTTT TCTCGGAACT ATGACCATCG CCTACGGTTT 
GAAGATCGAA TCCCTACCTA TGAAGACCAG TGGTCATATT ATGATGACAA ACAGCCCTAC 
CAACCTCGGC CTTTTGAGAA TCAGCATCCC CGAGACCTGG ACTCCAGACA ACATCCNGAG 
GAGGCTTCAG AACGAGGTTA TNTCCAGCGT TTTGAAGAGC CAGCCCCTCT GTCATACGAC 
AGTAGAACAC GCTATGAGCA GCTGCCTCGA ACCTCTACTC TACGACATGA AGAGCAGCCA 
GCCCCTGCAT ATGAGGTGCA CAACAGGTAC AGGCCAGAGG CACAGCCCTA TTCTTCAACA 
GGTCCTAAGT CATCTGAGCC CAAGCAGTAC TTTGACCAGT ACCCGCGAAG TTATGAGCAA 
GTACCACCAC CAGGCTTTAC CTCCAAAACA GGCCATTACG AGCCTCTCCA TGGTGCTGCA 
GTTGTCCCTC CTCTGATACC TTCCTCTCAA CATAAGCCAG AAGTCCTGCC CTCGGCTACC 
AAACCACAGC CTCCACCCCC AACCCTAACT GAGGAGGAGG AGGATCCAGC AATGAAACCA 
CAGTCCGTGC TCANCAGAGT CAAAATGTTT GAAAACAAAA GATCTGCGTC TTTGGAGAAC 
AAGAAAGATG TGAATGACAC TGCCAGCTTT AAGCCTCCAG AAGTAGCATC TAAACCTCCA 
AGTGCTTCTC TTGCTGGCCC TAAACCTGTT CCTCAGAGTC AGTTTAGTGA GCANGACAAA 
ACACTCTACA GGCTCCCAGA GCCTCAGAAA CCTCAAGTGA AGCCACCCGA NGATATTGTT 
CGATCAAATC ATTACGACCC TGAAGAGGAT GAAGAATATT ACAGGAAACA GCTCTCTTAC 
TTTGANCGAA GAAGTTTTGA GAGCAAGCCT TCTGCACATC TTCCTGCTGG CCATCACTCA 
GAGCCTGCTA AGCCAGTCCA TTCTCAGAGC CAGCCCAACT TTTCTAGTTA CTCTTCAAAG 
GGAAAACCCG AAACTGATGC TGTGGATAGA TCATTCAGTG AGAAACGTTA TGATCCAGCC 
CAGGCCACGC CTCCTCCTCC TCCGTTGCCC TCACAGTACA GCCAGCCAGC TCCCCCTCTG 
TCCAGCTCTT CTCTCCACAT ACATTCCAAG GGCGCCCAGG GCGAAGGCAA TTCAGTATCA 
TTGGATTTTC AGAACTCATA TATGTCCAAA CCAGACCCAC CCCCATCTCA GAGTAAACCA 
GCAACTTTCA GACCACCAAC CCGAGAAGAC CCTCCTCAGA CTTTCTATCC CCAGAAANGT 
TTCCCAGACA AAGCTCCAGT TAANGGAGCT GAGCAGACTC AGAAAACCAT CACTCCAGTG 
TACAATCGAT TCACACCAAA GCCGTACACA AGTTCTGCCC GGCCGTTTGA ACGCAAATTT 
GAAAGTCCGA AGTTCAACCA TAATCTTCTG CCAAGTGAAA CTGTACATAA ACCTGAATTG 
TCTTCAAAAA CTCCCACTTC TCCAAAAACT CTTATGAAAG CTCATAGTTC AACACAGCCT 
CCAGAGTTTG ACAGTGGAGT TGAAACTTTC TCTGTTCACA CAGATNAGCC TAAATATCAA 
ATGAATAATA TCAGCACCAT GCCTAAAGCT GTCCCTGTGA GTCCTTCAGC TGTGGAAGAA 
GATGAAGATG AGGATGGTCA TACTGTAGTG GCTACAGCTC GTGGCATTTT TAACAGCAAT 
GGTGGTGTGT TGAGTTCCAT AGAAACCGGT GTTAGTATAA TTATCCCACA AGGAGCCATT 
CCTGAAGGAA TTGAACAAGA AATCTATTTC AAAGTCTGCA GAGACAATAG CATTCTCCCA 
CCTTTAGATA AAGAGAAAGG TGAAACTCTG CTAAGTCCCC TAGTGATGTG TGGGCCCCAT 
GGCCTCAAGT TCCTGAAGCC CGTGGAGCTG CGCTTGCCAC ACTGTGACCC TAAAACCTGG 
CAAAACAAGT GTCTTCCTGG AGATCCGAAT TACCTTGTTG GAGCCAACTG TGTTTCTGTC 
CTGATTGACC ACTTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021021826.1
CDS	38..5035
Translation	MSTRAAAAKSTAMEETAIWEQHTVMLHRAPGFGFGIAISGGRDNPHFQSGETSIVISDVLKGGPAEGQLQENDRVAMVNGVSMDNVEHAFAVQQLRKSGKNAKITMRRKKKVQIPVSHPDPEPVSDNEDDSYDEEVHDPRAGRSALANRRSEKSWARDRSASRERSLSPRSDRRSVASSQPAKPTKVALVKSRKNEEYGLRLASHIFVKEISQDSLAARDGNIQEGDVVLKINGTVTENMSLTDAKTLIERSKGKLKMVVQRDERATLLNVPDLSDSIHSANASERDDISEIQSLASDHSGRSHDRPPRRSQSRSPDQRSEPSDHSTQSPQQPSNGSLRSREQERMSKPGAISTPVKHADDHTPKAVEEVTVEKNEKQTPTLPEPKPVYAQVGQPDVDLPVSPSDGVLPNSTHEDGILRPSMKLVKFRKGDSVGLRLAGGNDVGIFVAGVLEDSPAAKEGLEEGDQILRVNNVDFTNIIREEAVLFLLDLPKGEEVTILAQKKKGVYRRIVESDVGDSFYIRTHFEYEKESPYGLSFNKGEVFRVVDTLYNGKLGSWLAIRIGKNHKEVERGIIPNKNRAEQLASVQYTLPKTAGGDRADFWRFRGLRSSKRNLRKSREDLSAQPVQTKFPAYERVVLREAGFLRPVTIFGPIADVAREKLAREEPDIYQIAKSEPRDAGTDHRSSGIIRLHTIKQIIDQDKHALLDVTPNAVDRLNYAQWYPIVVFLNPDSKQGVKTMRMRLCPESRKSARKLYERSHKLRKNNHHLFTTTINLNSMNDGWYGALKEAIQQQQNQLVWVSEGKADGATSDDLDLHDDRLSYLSAPGSEYSMYSTDSRHTSDYEDTDTEGGAYTDQELDETLNDEVGTPPESAITnullSSEPVREDSSGMHHENQTYPPYSPQAQPQAIHRLDSPGLKPASQQVYRKEPYPEEMMRQNHILKQPALGHPGQRPDKEPNLAYEPQLPYnullEKQASRDLEQPSYRYEASSYPDQFSRNYDHRLRFEDRIPTYEDQWSYYDDKQPYQPRPFENQHPRDLDSRQHnullEEASERGYnullQRFEEPAPLSYDSRTRYEQLPRTSTLRHEEQPAPAYEVHNRYRPEAQPYSSTGPKSSEPKQYFDQYPRSYEQVPPPGFTSKTGHYEPLHGAAVVPPLIPSSQHKPEVLPSATKPQPPPPTLTEEEEDPAMKPQSVLnullRVKMFENKRSASLENKKDVNDTASFKPPEVASKPPSASLAGPKPVPQSQFSEnullDKTLYRLPEPQKPQVKPPnullDIVRSNHYDPEEDEEYYRKQLSYFnullRRSFESKPSAHLPAGHHSEPAKPVHSQSQPNFSSYSSKGKPETDAVDRSFSEKRYDPAQATPPPPPLPSQYSQPAPPLSSSSLHIHSKGAQGEGNSVSLDFQNSYMSKPDPPPSQSKPATFRPPTREDPPQTFYPQKnullFPDKAPVnullGAEQTQKTITPVYNRFTPKPYTSSARPFERKFESPKFNHNLLPSETVHKPELSSKTPTSPKTLMKAHSSTQPPEFDSGVETFSVHTDnullPKYQMNNISTMPKAVPVSPSAVEEDEDEDGHTVVATARGIFNSNGGVLSSIETGVSIIIPQGAIPEGIEQEIYFKVCRDNSILPPLDKEKGETLLSPLVMCGPHGLKFLKPVELRLPHCDPKTWQNKCLPGDPNYLVGANCVSVLIDHF

Target ORF information:

RefSeq Version	XM_021166167.1
Organism	Mus caroli(Ryukyu mouse)
Definition	Mus caroli tight junction protein 1 (Tjp1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021166167.1

ORF Insert Sequence:

ATGTCCACCA GGGCCGCGGC CGCTAAGAGC ACAGCAATGG AGGAAACGGC TATATGGGAA 
CAGCACACAG TGATGCTTCA CAGGGCTCCT GGGTTTGGAT TTGGAATTGC AATCTCTGGT 
GGAAGAGATA ATCCTCATTT TCAGAGTGGG GAAACCTCCA TAGTGATTTC TGATGTGCTA 
AAAGGAGGGC CAGCTGAAGG ACAGCTACAG GAAAATGACC GAGTTGCAAT GGTTAATGGA 
GTTTCAATGG ATAACGTTGA ACATGCTTTT GCTGTTCAGC AGCTAAGGAA AAGTGGGAAA 
AACGCAAAAA TTACTATGCG AAGGAAGAAG AAAGTTCAGA TCCCTGTAAG TCACCCAGAT 
CCTGAGCCGG TGTCTGATAA TGAAGATGAT AGCTACGACG AAGAAGTACA TGATCCAAGA 
GCTGGCCGCA GTGCTTTAGC GAACAGAAGG AGCGAGAAGA GCTGGGCAAG GGATAGGAGT 
GCAAGCAGGG AGAGGAGCCT GTCCCCTCGC TCGGACAGGC GGTCGGTGGC CTCCAGTCAG 
CCCGCAAAAC CCACCAAGGT CGCACTGGTG AAGTCTCGGA AAAATGAAGA ATATGGTCTT 
CGATTGGCCA GCCATATATT TGTAAAGGAA ATTTCACAAG ATAGTTTGGC AGCAAGAGAT 
GGTAACATTC AAGAAGGGGA TGTTGTCTTG AAGATAAATG GTACTGTGAC AGAAAATATG 
TCATTGACAG ATGCAAAAAC ACTGATAGAA AGGTCTAAAG GCAAGTTAAA AATGGTAGTG 
CAAAGAGATG AGCGGGCTAC CTTACTGAAC GTCCCTGACC TTTCGGATAG TATCCATTCT 
GCTAATGCCT CGGAAAGAGA TGACATTTCA GAAATTCAGT CACTAGCATC AGACCATTCA 
GGTCGCTCGC ATGACAGGCC ACCCCGCCGC AGCCAGTCAC GATCTCCTGA CCAACGTTCA 
GAGCCCTCCG ATCATTCCAC ACAGTCTCCA CAGCAGCCCA GCAATGGCAG TCTCCGGAGC 
AGAGAGCAAG AGCGAATGTC TAAACCTGGG GCCATCTCAA CTCCTGTAAA ACATGCAGAT 
GATCATACAC CCAAAGCAGT GGAAGAAGTT ACAGTTGAGA AAAATGAGAA GCAGACACCC 
ACTCTTCCAG AACCGAAGCC TGTATATGCT CAAGTTGGAC AACCAGATGT GGATTTACCC 
GTCAGCCCTT CTGATGGTGT TCTGCCTAAT TCAACTCATG AAGATGGGAT ACTTAGGCCC 
AGCATGAAAC TGGTAAAATT CAGAAAAGGA GATAGTGTGG GTTTGCGACT AGCTGGTGGA 
AATGATGTCG GAATATTTGT AGCTGGCGTT CTAGAAGATA GCCCTGCAGC CAAAGAAGGC 
TTAGAGGAAG GTGATCAAAT TCTCAGGGTG AACAATGTAG ATTTCACAAA TATCATAAGA 
GAAGAGGCTG TCCTTTTCCT CCTTGACCTC CCTAAAGGTG AAGAAGTGAC CATCCTGGCT 
CAGAAGAAGA AGGGTGTTTA TCGCCGCATT GTAGAATCAG ATGTAGGAGA TTCATTCTAT 
ATTAGAACGC ATTTTGAGTA TGAAAAAGAA TCTCCTTACG GACTTAGTTT TAACAAAGGA 
GAGGTGTTCC GGGTCGTGGA TACTTTATAC AATGGAAAGC TGGGCTCTTG GCTTGCCATT 
CGAATTGGCA AAAATCATAA GGAGGTAGAA CGAGGCATCA TCCCTAATAA GAACAGAGCT 
GAGCAGTTAG CCAGTGTACA GTACACACTT CCAAAGACAG CGGGTGGTGA TCGGGCAGAC 
TTCTGGAGGT TTCGAGGTCT TCGCAGCTCC AAGAGAAATC TTCGAAAAAG CAGAGAGGAC 
TTGTCAGCTC AGCCAGTTCA AACTAAGTTC CCAGCTTATG AAAGGGTTGT TCTTCGAGAA 
GCTGGATTCC TAAGACCTGT AACCATCTTT GGACCAATAG CTGATGTTGC CAGAGAAAAG 
TTGGCAAGAG AGGAACCAGA TATCTATCAG ATTGCAAAAA GTGAACCACG AGATGCTGGG 
ACTGACCATC GTAGCTCTGG CATCATTCGC CTTCATACAA TAAAGCAAAT CATAGATCAG 
GATAAACATG CGTTATTAGA TGTAACGCCA AATGCGGTTG ATCGTCTTAA TTATGCGCAG 
TGGTATCCAA TTGTTGTGTT CCTTAACCCT GACTCTAAGC AAGGTGTAAA AACAATGAGG 
ATGAGGTTAT GTCCGGAGTC TCGGAAAAGC GCCAGGAAGC TATATGAACG CTCTCATAAG 
CTTCGCAAAA ACAATCACCA CCTTTTCACA ACTACAATTA ACTTAAACTC AATGAATGAT 
GGTTGGTACG GTGCCCTGAA AGAAGCCATT CAGCAGCAAC AGAACCAGCT GGTGTGGGTC 
TCTGAGGGGA AGGCGGATGG TGCTACAAGT GATGACCTTG ATTTGCATGA TGATCGTCTG 
TCCTACCTGT CAGCCCCAGG TAGTGAGTAC TCAATGTATA GCACGGACAG TAGACACACT 
TCTGACTATG AAGACACAGA TACAGAAGGC GGGGCCTACA CTGATCAAGA ACTAGATGAA 
ACTCTTAATG ATGAGGTGGG GACTCCCCCG GAGTCTGCCA TTACACNGTC CTCTGAGCCT 
GTAAGAGAGG ATTCCTCTGG GATGCATCAT GAAAACCAGA CCTATCCTCC TTACTCACCA 
CAAGCGCAGC CACAAGCTAT TCATAGACTA GACTCCCCTG GACTTAAGCC AGCCTCTCAA 
CAGGTGTATA GAAAGGAGCC GTATCCTGAA GAAATGATGA GACAAAACCA TATTTTAAAA 
CAACCAGCTC TTGGTCACCC AGGGCAGAGG CCAGATAAAG AGCCAAATCT AGCCTATGAA 
CCCCAACTTC CATATANAGA AAAACAAGCC AGCAGAGACC TTGAACAGCC GTCATACAGG 
TATGAGGCCT CAAGCTACCC AGACCAGTTT TCTCGGAACT ATGACCATCG CCTACGGTTT 
GAAGATCGAA TCCCTACCTA TGAAGACCAG TGGTCATATT ATGATGACAA ACAGCCCTAC 
CAACCTCGGC CTTTTGAGAA TCAGCATCCC CGAGACCTGG ACTCCAGACA ACATCCNGAG 
GAGGCTTCAG AACGAGGTTA TNTCCAGCGT TTTGAAGAGC CAGCCCCTCT GTCATACGAC 
AGTAGAACAC GCTATGAGCA GCTGCCTCGA ACCTCTACTC TACGACATGA AGAGCAGCCA 
GCCCCTGCAT ATGAGGTGCA CAACAGGTAC AGGCCAGAGG CACAGCCCTA TTCTTCAACA 
GGTCCTAAGT CATCTGAGCC CAAGCAGTAC TTTGACCAGT ACCCGCGAAG TTATGAGCAA 
GTACCACCAC CAGGCTTTAC CTCCAAAACA GGCCATTACG AGCCTCTCCA TGGTGCTGCA 
GTTGTCCCTC CTCTGATACC TTCCTCTCAA CATAAGCCAG AAGTCCTGCC CTCGGCTACC 
AAACCACAGC CTCCACCCCC AACCCTAACT GAGGAGGAGG AGGATCCAGC AATGAAACCA 
CAGTCCGTGC TCANCAGAGT CAAAATGTTT GAAAACAAAA GATCTGCGTC TTTGGAGAAC 
AAGAAAGATG TGAATGACAC TGCCAGCTTT AAGCCTCCAG AAGTAGCATC TAAACCTCCA 
AGTGCTTCTC TTGCTGGCCC TAAACCTGTT CCTCAGAGTC AGTTTAGTGA GCANGACAAA 
ACACTCTACA GGCTCCCAGA GCCTCAGAAA CCTCAAGTGA AGCCACCCGA NGATATTGTT 
CGATCAAATC ATTACGACCC TGAAGAGGAT GAAGAATATT ACAGGAAACA GCTCTCTTAC 
TTTGANCGAA GAAGTTTTGA GAGCAAGCCT TCTGCACATC TTCCTGCTGG CCATCACTCA 
GAGCCTGCTA AGCCAGTCCA TTCTCAGAGC CAGCCCAACT TTTCTAGTTA CTCTTCAAAG 
GGAAAACCCG AAACTGATGC TGTGGATAGA TCATTCAGTG AGAAACGTTA TGATCCAGCC 
CAGGCCACGC CTCCTCCTCC TCCGTTGCCC TCACAGTACA GCCAGCCAGC TCCCCCTCTG 
TCCAGCTCTT CTCTCCACAT ACATTCCAAG GGCGCCCAGG GCGAAGGCAA TTCAGTATCA 
TTGGATTTTC AGAACTCATA TATGTCCAAA CCAGACCCAC CCCCATCTCA GAGTAAACCA 
GCAACTTTCA GACCACCAAC CCGAGAAGAC CCTCCTCAGA CTTTCTATCC CCAGAAANGT 
TTCCCAGACA AAGCTCCAGT TAANGGAGCT GAGCAGACTC AGAAAACCAT CACTCCAGTG 
TACAATCGAT TCACACCAAA GCCGTACACA AGTTCTGCCC GGCCGTTTGA ACGCAAATTT 
GAAAGTCCGA AGTTCAACCA TAATCTTCTG CCAAGTGAAA CTGTACATAA ACCTGAATTG 
TCTTCAAAAA CTCCCACTTC TCCAAAAACT CTTATGAAAG CTCATAGTTC AACACAGCCT 
CCAGAGTTTG ACAGTGGAGT TGAAACTTTC TCTGTTCACA CAGATNAGCC TAAATATCAA 
ATGAATAATA TCAGCACCAT GCCTAAAGCT GTCCCTGTGA GTCCTTCAGC TGTGGAAGAA 
GATGAAGATG AGGATGGTCA TACTGTAGTG GCTACAGCTC GTGGCATTTT TAACAGCAAT 
GGTGGTGTGT TGAGTTCCAT AGAAACCGGT GTTAGTATAA TTATCCCACA AGGAGCCATT 
CCTGAAGGAA TTGAACAAGA AATCTATTTC AAAGTCTGCA GAGACAATAG CATTCTCCCA 
CCTTTAGATA AAGAGAAAGG TGAAACTCTG CTAAGTCCCC TAGTGATGTG TGGGCCCCAT 
GGCCTCAAGT TCCTGAAGCC CGTGGAGCTG CGCTTGCCAC ACTGTGACCC TAAAACCTGG 
CAAAACAAGT GTCTTCCTGG AGATCCGAAT TACCTTGTTG GAGCCAACTG TGTTTCTGTC 
CTGATTGACC ACTTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Tjp1 ( XM_021166167.2 ) cDNA ORF clone, Mus caroli(Ryukyu mouse) -> XP_021021826.1 Mus caroli tight junction protein 1 (Tjp1), transcript variant X10, mRNA.

Price & Availability↑

CloneID	OMv03981
Clone ID Related Accession (Same CDS sequence)	XM_021166167.1 , XM_021166167.2
Accession Version	XM_021166167.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4998bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-06-09
Organism	Mus caroli(Ryukyu mouse)
Product	tight junction protein ZO-1 isoform X10
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_034576.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process On Jun 10, 2019 this sequence version replaced XM_021166167.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus caroli Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCACCA GGGCCGCGGC CGCTAAGAGC ACAGCAATGG AGGAAACGGC TATATGGGAA 
CAGCACACAG TGATGCTTCA CAGGGCTCCT GGGTTTGGAT TTGGAATTGC AATCTCTGGT 
GGAAGAGATA ATCCTCATTT TCAGAGTGGG GAAACCTCCA TAGTGATTTC TGATGTGCTA 
AAAGGAGGGC CAGCTGAAGG ACAGCTACAG GAAAATGACC GAGTTGCAAT GGTTAATGGA 
GTTTCAATGG ATAACGTTGA ACATGCTTTT GCTGTTCAGC AGCTAAGGAA AAGTGGGAAA 
AACGCAAAAA TTACTATGCG AAGGAAGAAG AAAGTTCAGA TCCCTGTAAG TCACCCAGAT 
CCTGAGCCGG TGTCTGATAA TGAAGATGAT AGCTACGACG AAGAAGTACA TGATCCAAGA 
GCTGGCCGCA GTGCTTTAGC GAACAGAAGG AGCGAGAAGA GCTGGGCAAG GGATAGGAGT 
GCAAGCAGGG AGAGGAGCCT GTCCCCTCGC TCGGACAGGC GGTCGGTGGC CTCCAGTCAG 
CCCGCAAAAC CCACCAAGGT CGCACTGGTG AAGTCTCGGA AAAATGAAGA ATATGGTCTT 
CGATTGGCCA GCCATATATT TGTAAAGGAA ATTTCACAAG ATAGTTTGGC AGCAAGAGAT 
GGTAACATTC AAGAAGGGGA TGTTGTCTTG AAGATAAATG GTACTGTGAC AGAAAATATG 
TCATTGACAG ATGCAAAAAC ACTGATAGAA AGGTCTAAAG GCAAGTTAAA AATGGTAGTG 
CAAAGAGATG AGCGGGCTAC CTTACTGAAC GTCCCTGACC TTTCGGATAG TATCCATTCT 
GCTAATGCCT CGGAAAGAGA TGACATTTCA GAAATTCAGT CACTAGCATC AGACCATTCA 
GGTCGCTCGC ATGACAGGCC ACCCCGCCGC AGCCAGTCAC GATCTCCTGA CCAACGTTCA 
GAGCCCTCCG ATCATTCCAC ACAGTCTCCA CAGCAGCCCA GCAATGGCAG TCTCCGGAGC 
AGAGAGCAAG AGCGAATGTC TAAACCTGGG GCCATCTCAA CTCCTGTAAA ACATGCAGAT 
GATCATACAC CCAAAGCAGT GGAAGAAGTT ACAGTTGAGA AAAATGAGAA GCAGACACCC 
ACTCTTCCAG AACCGAAGCC TGTATATGCT CAAGTTGGAC AACCAGATGT GGATTTACCC 
GTCAGCCCTT CTGATGGTGT TCTGCCTAAT TCAACTCATG AAGATGGGAT ACTTAGGCCC 
AGCATGAAAC TGGTAAAATT CAGAAAAGGA GATAGTGTGG GTTTGCGACT AGCTGGTGGA 
AATGATGTCG GAATATTTGT AGCTGGCGTT CTAGAAGATA GCCCTGCAGC CAAAGAAGGC 
TTAGAGGAAG GTGATCAAAT TCTCAGGGTG AACAATGTAG ATTTCACAAA TATCATAAGA 
GAAGAGGCTG TCCTTTTCCT CCTTGACCTC CCTAAAGGTG AAGAAGTGAC CATCCTGGCT 
CAGAAGAAGA AGGGTGTTTA TCGCCGCATT GTAGAATCAG ATGTAGGAGA TTCATTCTAT 
ATTAGAACGC ATTTTGAGTA TGAAAAAGAA TCTCCTTACG GACTTAGTTT TAACAAAGGA 
GAGGTGTTCC GGGTCGTGGA TACTTTATAC AATGGAAAGC TGGGCTCTTG GCTTGCCATT 
CGAATTGGCA AAAATCATAA GGAGGTAGAA CGAGGCATCA TCCCTAATAA GAACAGAGCT 
GAGCAGTTAG CCAGTGTACA GTACACACTT CCAAAGACAG CGGGTGGTGA TCGGGCAGAC 
TTCTGGAGGT TTCGAGGTCT TCGCAGCTCC AAGAGAAATC TTCGAAAAAG CAGAGAGGAC 
TTGTCAGCTC AGCCAGTTCA AACTAAGTTC CCAGCTTATG AAAGGGTTGT TCTTCGAGAA 
GCTGGATTCC TAAGACCTGT AACCATCTTT GGACCAATAG CTGATGTTGC CAGAGAAAAG 
TTGGCAAGAG AGGAACCAGA TATCTATCAG ATTGCAAAAA GTGAACCACG AGATGCTGGG 
ACTGACCATC GTAGCTCTGG CATCATTCGC CTTCATACAA TAAAGCAAAT CATAGATCAG 
GATAAACATG CGTTATTAGA TGTAACGCCA AATGCGGTTG ATCGTCTTAA TTATGCGCAG 
TGGTATCCAA TTGTTGTGTT CCTTAACCCT GACTCTAAGC AAGGTGTAAA AACAATGAGG 
ATGAGGTTAT GTCCGGAGTC TCGGAAAAGC GCCAGGAAGC TATATGAACG CTCTCATAAG 
CTTCGCAAAA ACAATCACCA CCTTTTCACA ACTACAATTA ACTTAAACTC AATGAATGAT 
GGTTGGTACG GTGCCCTGAA AGAAGCCATT CAGCAGCAAC AGAACCAGCT GGTGTGGGTC 
TCTGAGGGGA AGGCGGATGG TGCTACAAGT GATGACCTTG ATTTGCATGA TGATCGTCTG 
TCCTACCTGT CAGCCCCAGG TAGTGAGTAC TCAATGTATA GCACGGACAG TAGACACACT 
TCTGACTATG AAGACACAGA TACAGAAGGC GGGGCCTACA CTGATCAAGA ACTAGATGAA 
ACTCTTAATG ATGAGGTGGG GACTCCCCCG GAGTCTGCCA TTACACNGTC CTCTGAGCCT 
GTAAGAGAGG ATTCCTCTGG GATGCATCAT GAAAACCAGA CCTATCCTCC TTACTCACCA 
CAAGCGCAGC CACAAGCTAT TCATAGACTA GACTCCCCTG GACTTAAGCC AGCCTCTCAA 
CAGGTGTATA GAAAGGAGCC GTATCCTGAA GAAATGATGA GACAAAACCA TATTTTAAAA 
CAACCAGCTC TTGGTCACCC AGGGCAGAGG CCAGATAAAG AGCCAAATCT AGCCTATGAA 
CCCCAACTTC CATATANAGA AAAACAAGCC AGCAGAGACC TTGAACAGCC GTCATACAGG 
TATGAGGCCT CAAGCTACCC AGACCAGTTT TCTCGGAACT ATGACCATCG CCTACGGTTT 
GAAGATCGAA TCCCTACCTA TGAAGACCAG TGGTCATATT ATGATGACAA ACAGCCCTAC 
CAACCTCGGC CTTTTGAGAA TCAGCATCCC CGAGACCTGG ACTCCAGACA ACATCCNGAG 
GAGGCTTCAG AACGAGGTTA TNTCCAGCGT TTTGAAGAGC CAGCCCCTCT GTCATACGAC 
AGTAGAACAC GCTATGAGCA GCTGCCTCGA ACCTCTACTC TACGACATGA AGAGCAGCCA 
GCCCCTGCAT ATGAGGTGCA CAACAGGTAC AGGCCAGAGG CACAGCCCTA TTCTTCAACA 
GGTCCTAAGT CATCTGAGCC CAAGCAGTAC TTTGACCAGT ACCCGCGAAG TTATGAGCAA 
GTACCACCAC CAGGCTTTAC CTCCAAAACA GGCCATTACG AGCCTCTCCA TGGTGCTGCA 
GTTGTCCCTC CTCTGATACC TTCCTCTCAA CATAAGCCAG AAGTCCTGCC CTCGGCTACC 
AAACCACAGC CTCCACCCCC AACCCTAACT GAGGAGGAGG AGGATCCAGC AATGAAACCA 
CAGTCCGTGC TCANCAGAGT CAAAATGTTT GAAAACAAAA GATCTGCGTC TTTGGAGAAC 
AAGAAAGATG TGAATGACAC TGCCAGCTTT AAGCCTCCAG AAGTAGCATC TAAACCTCCA 
AGTGCTTCTC TTGCTGGCCC TAAACCTGTT CCTCAGAGTC AGTTTAGTGA GCANGACAAA 
ACACTCTACA GGCTCCCAGA GCCTCAGAAA CCTCAAGTGA AGCCACCCGA NGATATTGTT 
CGATCAAATC ATTACGACCC TGAAGAGGAT GAAGAATATT ACAGGAAACA GCTCTCTTAC 
TTTGANCGAA GAAGTTTTGA GAGCAAGCCT TCTGCACATC TTCCTGCTGG CCATCACTCA 
GAGCCTGCTA AGCCAGTCCA TTCTCAGAGC CAGCCCAACT TTTCTAGTTA CTCTTCAAAG 
GGAAAACCCG AAACTGATGC TGTGGATAGA TCATTCAGTG AGAAACGTTA TGATCCAGCC 
CAGGCCACGC CTCCTCCTCC TCCGTTGCCC TCACAGTACA GCCAGCCAGC TCCCCCTCTG 
TCCAGCTCTT CTCTCCACAT ACATTCCAAG GGCGCCCAGG GCGAAGGCAA TTCAGTATCA 
TTGGATTTTC AGAACTCATA TATGTCCAAA CCAGACCCAC CCCCATCTCA GAGTAAACCA 
GCAACTTTCA GACCACCAAC CCGAGAAGAC CCTCCTCAGA CTTTCTATCC CCAGAAANGT 
TTCCCAGACA AAGCTCCAGT TAANGGAGCT GAGCAGACTC AGAAAACCAT CACTCCAGTG 
TACAATCGAT TCACACCAAA GCCGTACACA AGTTCTGCCC GGCCGTTTGA ACGCAAATTT 
GAAAGTCCGA AGTTCAACCA TAATCTTCTG CCAAGTGAAA CTGTACATAA ACCTGAATTG 
TCTTCAAAAA CTCCCACTTC TCCAAAAACT CTTATGAAAG CTCATAGTTC AACACAGCCT 
CCAGAGTTTG ACAGTGGAGT TGAAACTTTC TCTGTTCACA CAGATNAGCC TAAATATCAA 
ATGAATAATA TCAGCACCAT GCCTAAAGCT GTCCCTGTGA GTCCTTCAGC TGTGGAAGAA 
GATGAAGATG AGGATGGTCA TACTGTAGTG GCTACAGCTC GTGGCATTTT TAACAGCAAT 
GGTGGTGTGT TGAGTTCCAT AGAAACCGGT GTTAGTATAA TTATCCCACA AGGAGCCATT 
CCTGAAGGAA TTGAACAAGA AATCTATTTC AAAGTCTGCA GAGACAATAG CATTCTCCCA 
CCTTTAGATA AAGAGAAAGG TGAAACTCTG CTAAGTCCCC TAGTGATGTG TGGGCCCCAT 
GGCCTCAAGT TCCTGAAGCC CGTGGAGCTG CGCTTGCCAC ACTGTGACCC TAAAACCTGG 
CAAAACAAGT GTCTTCCTGG AGATCCGAAT TACCTTGTTG GAGCCAACTG TGTTTCTGTC 
CTGATTGACC ACTTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021021826.1
CDS	65..5062
Translation	MSTRAAAAKSTAMEETAIWEQHTVMLHRAPGFGFGIAISGGRDNPHFQSGETSIVISDVLKGGPAEGQLQENDRVAMVNGVSMDNVEHAFAVQQLRKSGKNAKITMRRKKKVQIPVSHPDPEPVSDNEDDSYDEEVHDPRAGRSALANRRSEKSWARDRSASRERSLSPRSDRRSVASSQPAKPTKVALVKSRKNEEYGLRLASHIFVKEISQDSLAARDGNIQEGDVVLKINGTVTENMSLTDAKTLIERSKGKLKMVVQRDERATLLNVPDLSDSIHSANASERDDISEIQSLASDHSGRSHDRPPRRSQSRSPDQRSEPSDHSTQSPQQPSNGSLRSREQERMSKPGAISTPVKHADDHTPKAVEEVTVEKNEKQTPTLPEPKPVYAQVGQPDVDLPVSPSDGVLPNSTHEDGILRPSMKLVKFRKGDSVGLRLAGGNDVGIFVAGVLEDSPAAKEGLEEGDQILRVNNVDFTNIIREEAVLFLLDLPKGEEVTILAQKKKGVYRRIVESDVGDSFYIRTHFEYEKESPYGLSFNKGEVFRVVDTLYNGKLGSWLAIRIGKNHKEVERGIIPNKNRAEQLASVQYTLPKTAGGDRADFWRFRGLRSSKRNLRKSREDLSAQPVQTKFPAYERVVLREAGFLRPVTIFGPIADVAREKLAREEPDIYQIAKSEPRDAGTDHRSSGIIRLHTIKQIIDQDKHALLDVTPNAVDRLNYAQWYPIVVFLNPDSKQGVKTMRMRLCPESRKSARKLYERSHKLRKNNHHLFTTTINLNSMNDGWYGALKEAIQQQQNQLVWVSEGKADGATSDDLDLHDDRLSYLSAPGSEYSMYSTDSRHTSDYEDTDTEGGAYTDQELDETLNDEVGTPPESAITnullSSEPVREDSSGMHHENQTYPPYSPQAQPQAIHRLDSPGLKPASQQVYRKEPYPEEMMRQNHILKQPALGHPGQRPDKEPNLAYEPQLPYnullEKQASRDLEQPSYRYEASSYPDQFSRNYDHRLRFEDRIPTYEDQWSYYDDKQPYQPRPFENQHPRDLDSRQHnullEEASERGYnullQRFEEPAPLSYDSRTRYEQLPRTSTLRHEEQPAPAYEVHNRYRPEAQPYSSTGPKSSEPKQYFDQYPRSYEQVPPPGFTSKTGHYEPLHGAAVVPPLIPSSQHKPEVLPSATKPQPPPPTLTEEEEDPAMKPQSVLnullRVKMFENKRSASLENKKDVNDTASFKPPEVASKPPSASLAGPKPVPQSQFSEnullDKTLYRLPEPQKPQVKPPnullDIVRSNHYDPEEDEEYYRKQLSYFnullRRSFESKPSAHLPAGHHSEPAKPVHSQSQPNFSSYSSKGKPETDAVDRSFSEKRYDPAQATPPPPPLPSQYSQPAPPLSSSSLHIHSKGAQGEGNSVSLDFQNSYMSKPDPPPSQSKPATFRPPTREDPPQTFYPQKnullFPDKAPVnullGAEQTQKTITPVYNRFTPKPYTSSARPFERKFESPKFNHNLLPSETVHKPELSSKTPTSPKTLMKAHSSTQPPEFDSGVETFSVHTDnullPKYQMNNISTMPKAVPVSPSAVEEDEDEDGHTVVATARGIFNSNGGVLSSIETGVSIIIPQGAIPEGIEQEIYFKVCRDNSILPPLDKEKGETLLSPLVMCGPHGLKFLKPVELRLPHCDPKTWQNKCLPGDPNYLVGANCVSVLIDHF

Target ORF information:

RefSeq Version	XM_021166167.2
Organism	Mus caroli(Ryukyu mouse)
Definition	Mus caroli tight junction protein 1 (Tjp1), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021166167.2

ORF Insert Sequence:

ATGTCCACCA GGGCCGCGGC CGCTAAGAGC ACAGCAATGG AGGAAACGGC TATATGGGAA 
CAGCACACAG TGATGCTTCA CAGGGCTCCT GGGTTTGGAT TTGGAATTGC AATCTCTGGT 
GGAAGAGATA ATCCTCATTT TCAGAGTGGG GAAACCTCCA TAGTGATTTC TGATGTGCTA 
AAAGGAGGGC CAGCTGAAGG ACAGCTACAG GAAAATGACC GAGTTGCAAT GGTTAATGGA 
GTTTCAATGG ATAACGTTGA ACATGCTTTT GCTGTTCAGC AGCTAAGGAA AAGTGGGAAA 
AACGCAAAAA TTACTATGCG AAGGAAGAAG AAAGTTCAGA TCCCTGTAAG TCACCCAGAT 
CCTGAGCCGG TGTCTGATAA TGAAGATGAT AGCTACGACG AAGAAGTACA TGATCCAAGA 
GCTGGCCGCA GTGCTTTAGC GAACAGAAGG AGCGAGAAGA GCTGGGCAAG GGATAGGAGT 
GCAAGCAGGG AGAGGAGCCT GTCCCCTCGC TCGGACAGGC GGTCGGTGGC CTCCAGTCAG 
CCCGCAAAAC CCACCAAGGT CGCACTGGTG AAGTCTCGGA AAAATGAAGA ATATGGTCTT 
CGATTGGCCA GCCATATATT TGTAAAGGAA ATTTCACAAG ATAGTTTGGC AGCAAGAGAT 
GGTAACATTC AAGAAGGGGA TGTTGTCTTG AAGATAAATG GTACTGTGAC AGAAAATATG 
TCATTGACAG ATGCAAAAAC ACTGATAGAA AGGTCTAAAG GCAAGTTAAA AATGGTAGTG 
CAAAGAGATG AGCGGGCTAC CTTACTGAAC GTCCCTGACC TTTCGGATAG TATCCATTCT 
GCTAATGCCT CGGAAAGAGA TGACATTTCA GAAATTCAGT CACTAGCATC AGACCATTCA 
GGTCGCTCGC ATGACAGGCC ACCCCGCCGC AGCCAGTCAC GATCTCCTGA CCAACGTTCA 
GAGCCCTCCG ATCATTCCAC ACAGTCTCCA CAGCAGCCCA GCAATGGCAG TCTCCGGAGC 
AGAGAGCAAG AGCGAATGTC TAAACCTGGG GCCATCTCAA CTCCTGTAAA ACATGCAGAT 
GATCATACAC CCAAAGCAGT GGAAGAAGTT ACAGTTGAGA AAAATGAGAA GCAGACACCC 
ACTCTTCCAG AACCGAAGCC TGTATATGCT CAAGTTGGAC AACCAGATGT GGATTTACCC 
GTCAGCCCTT CTGATGGTGT TCTGCCTAAT TCAACTCATG AAGATGGGAT ACTTAGGCCC 
AGCATGAAAC TGGTAAAATT CAGAAAAGGA GATAGTGTGG GTTTGCGACT AGCTGGTGGA 
AATGATGTCG GAATATTTGT AGCTGGCGTT CTAGAAGATA GCCCTGCAGC CAAAGAAGGC 
TTAGAGGAAG GTGATCAAAT TCTCAGGGTG AACAATGTAG ATTTCACAAA TATCATAAGA 
GAAGAGGCTG TCCTTTTCCT CCTTGACCTC CCTAAAGGTG AAGAAGTGAC CATCCTGGCT 
CAGAAGAAGA AGGGTGTTTA TCGCCGCATT GTAGAATCAG ATGTAGGAGA TTCATTCTAT 
ATTAGAACGC ATTTTGAGTA TGAAAAAGAA TCTCCTTACG GACTTAGTTT TAACAAAGGA 
GAGGTGTTCC GGGTCGTGGA TACTTTATAC AATGGAAAGC TGGGCTCTTG GCTTGCCATT 
CGAATTGGCA AAAATCATAA GGAGGTAGAA CGAGGCATCA TCCCTAATAA GAACAGAGCT 
GAGCAGTTAG CCAGTGTACA GTACACACTT CCAAAGACAG CGGGTGGTGA TCGGGCAGAC 
TTCTGGAGGT TTCGAGGTCT TCGCAGCTCC AAGAGAAATC TTCGAAAAAG CAGAGAGGAC 
TTGTCAGCTC AGCCAGTTCA AACTAAGTTC CCAGCTTATG AAAGGGTTGT TCTTCGAGAA 
GCTGGATTCC TAAGACCTGT AACCATCTTT GGACCAATAG CTGATGTTGC CAGAGAAAAG 
TTGGCAAGAG AGGAACCAGA TATCTATCAG ATTGCAAAAA GTGAACCACG AGATGCTGGG 
ACTGACCATC GTAGCTCTGG CATCATTCGC CTTCATACAA TAAAGCAAAT CATAGATCAG 
GATAAACATG CGTTATTAGA TGTAACGCCA AATGCGGTTG ATCGTCTTAA TTATGCGCAG 
TGGTATCCAA TTGTTGTGTT CCTTAACCCT GACTCTAAGC AAGGTGTAAA AACAATGAGG 
ATGAGGTTAT GTCCGGAGTC TCGGAAAAGC GCCAGGAAGC TATATGAACG CTCTCATAAG 
CTTCGCAAAA ACAATCACCA CCTTTTCACA ACTACAATTA ACTTAAACTC AATGAATGAT 
GGTTGGTACG GTGCCCTGAA AGAAGCCATT CAGCAGCAAC AGAACCAGCT GGTGTGGGTC 
TCTGAGGGGA AGGCGGATGG TGCTACAAGT GATGACCTTG ATTTGCATGA TGATCGTCTG 
TCCTACCTGT CAGCCCCAGG TAGTGAGTAC TCAATGTATA GCACGGACAG TAGACACACT 
TCTGACTATG AAGACACAGA TACAGAAGGC GGGGCCTACA CTGATCAAGA ACTAGATGAA 
ACTCTTAATG ATGAGGTGGG GACTCCCCCG GAGTCTGCCA TTACACNGTC CTCTGAGCCT 
GTAAGAGAGG ATTCCTCTGG GATGCATCAT GAAAACCAGA CCTATCCTCC TTACTCACCA 
CAAGCGCAGC CACAAGCTAT TCATAGACTA GACTCCCCTG GACTTAAGCC AGCCTCTCAA 
CAGGTGTATA GAAAGGAGCC GTATCCTGAA GAAATGATGA GACAAAACCA TATTTTAAAA 
CAACCAGCTC TTGGTCACCC AGGGCAGAGG CCAGATAAAG AGCCAAATCT AGCCTATGAA 
CCCCAACTTC CATATANAGA AAAACAAGCC AGCAGAGACC TTGAACAGCC GTCATACAGG 
TATGAGGCCT CAAGCTACCC AGACCAGTTT TCTCGGAACT ATGACCATCG CCTACGGTTT 
GAAGATCGAA TCCCTACCTA TGAAGACCAG TGGTCATATT ATGATGACAA ACAGCCCTAC 
CAACCTCGGC CTTTTGAGAA TCAGCATCCC CGAGACCTGG ACTCCAGACA ACATCCNGAG 
GAGGCTTCAG AACGAGGTTA TNTCCAGCGT TTTGAAGAGC CAGCCCCTCT GTCATACGAC 
AGTAGAACAC GCTATGAGCA GCTGCCTCGA ACCTCTACTC TACGACATGA AGAGCAGCCA 
GCCCCTGCAT ATGAGGTGCA CAACAGGTAC AGGCCAGAGG CACAGCCCTA TTCTTCAACA 
GGTCCTAAGT CATCTGAGCC CAAGCAGTAC TTTGACCAGT ACCCGCGAAG TTATGAGCAA 
GTACCACCAC CAGGCTTTAC CTCCAAAACA GGCCATTACG AGCCTCTCCA TGGTGCTGCA 
GTTGTCCCTC CTCTGATACC TTCCTCTCAA CATAAGCCAG AAGTCCTGCC CTCGGCTACC 
AAACCACAGC CTCCACCCCC AACCCTAACT GAGGAGGAGG AGGATCCAGC AATGAAACCA 
CAGTCCGTGC TCANCAGAGT CAAAATGTTT GAAAACAAAA GATCTGCGTC TTTGGAGAAC 
AAGAAAGATG TGAATGACAC TGCCAGCTTT AAGCCTCCAG AAGTAGCATC TAAACCTCCA 
AGTGCTTCTC TTGCTGGCCC TAAACCTGTT CCTCAGAGTC AGTTTAGTGA GCANGACAAA 
ACACTCTACA GGCTCCCAGA GCCTCAGAAA CCTCAAGTGA AGCCACCCGA NGATATTGTT 
CGATCAAATC ATTACGACCC TGAAGAGGAT GAAGAATATT ACAGGAAACA GCTCTCTTAC 
TTTGANCGAA GAAGTTTTGA GAGCAAGCCT TCTGCACATC TTCCTGCTGG CCATCACTCA 
GAGCCTGCTA AGCCAGTCCA TTCTCAGAGC CAGCCCAACT TTTCTAGTTA CTCTTCAAAG 
GGAAAACCCG AAACTGATGC TGTGGATAGA TCATTCAGTG AGAAACGTTA TGATCCAGCC 
CAGGCCACGC CTCCTCCTCC TCCGTTGCCC TCACAGTACA GCCAGCCAGC TCCCCCTCTG 
TCCAGCTCTT CTCTCCACAT ACATTCCAAG GGCGCCCAGG GCGAAGGCAA TTCAGTATCA 
TTGGATTTTC AGAACTCATA TATGTCCAAA CCAGACCCAC CCCCATCTCA GAGTAAACCA 
GCAACTTTCA GACCACCAAC CCGAGAAGAC CCTCCTCAGA CTTTCTATCC CCAGAAANGT 
TTCCCAGACA AAGCTCCAGT TAANGGAGCT GAGCAGACTC AGAAAACCAT CACTCCAGTG 
TACAATCGAT TCACACCAAA GCCGTACACA AGTTCTGCCC GGCCGTTTGA ACGCAAATTT 
GAAAGTCCGA AGTTCAACCA TAATCTTCTG CCAAGTGAAA CTGTACATAA ACCTGAATTG 
TCTTCAAAAA CTCCCACTTC TCCAAAAACT CTTATGAAAG CTCATAGTTC AACACAGCCT 
CCAGAGTTTG ACAGTGGAGT TGAAACTTTC TCTGTTCACA CAGATNAGCC TAAATATCAA 
ATGAATAATA TCAGCACCAT GCCTAAAGCT GTCCCTGTGA GTCCTTCAGC TGTGGAAGAA 
GATGAAGATG AGGATGGTCA TACTGTAGTG GCTACAGCTC GTGGCATTTT TAACAGCAAT 
GGTGGTGTGT TGAGTTCCAT AGAAACCGGT GTTAGTATAA TTATCCCACA AGGAGCCATT 
CCTGAAGGAA TTGAACAAGA AATCTATTTC AAAGTCTGCA GAGACAATAG CATTCTCCCA 
CCTTTAGATA AAGAGAAAGG TGAAACTCTG CTAAGTCCCC TAGTGATGTG TGGGCCCCAT 
GGCCTCAAGT TCCTGAAGCC CGTGGAGCTG CGCTTGCCAC ACTGTGACCC TAAAACCTGG 
CAAAACAAGT GTCTTCCTGG AGATCCGAAT TACCTTGTTG GAGCCAACTG TGTTTCTGTC 
CTGATTGACC ACTTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Tjp1 ( XM_029479116.1 ) cDNA ORF clone, Mus caroli(Ryukyu mouse) -> XP_029334976.1 Mus caroli tight junction protein 1 (Tjp1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMv74613
Clone ID Related Accession (Same CDS sequence)	XM_029479116.1
Accession Version	XM_029479116.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5499bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-06-09
Organism	Mus caroli(Ryukyu mouse)
Product	tight junction protein ZO-1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_034576.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus caroli Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGTACC AGAAATACCT GACGGTGCTG CAGATGGCCA TCGGCGTCAC CCCGTCCAAC 
CGCGGCAGCC TCCTGCCGCT CAAGAGGAAG CTGTGGGTAA CTCCATCCTC TGAAAACCCG 
CATGGTGCTA CTTCCAATGT CAGCCAAGGA AAACCTTCTC TAAGACGAAT TAAAGGGAGA 
CTACATCGAA GCAAAAGCCT TGACAGTATC GACTTCTGTG AACTCACTAG CACAGCAATG 
GAGGAAACGG CTATATGGGA ACAGCACACA GTGACGCTTC ACAGGGCTCC TGGGTTTGGA 
TTTGGAATTG CAATCTCTGG TGGAAGAGAT AATCCTCATT TTCAGAGTGG GGAAACCTCC 
ATAGTGATTT CTGATGTGCT AAAAGGAGGG CCAGCTGAAG GACAGCTACA GGAAAATGAC 
CGAGTTGCAA TGGTTAATGG AGTTTCAATG GATAACGTTG AGCATGCTTT TGCTGTTCAG 
CAGCTAAGGA AAAGTGGGAA AAACGCAAAA ATTACTATCC GAAGGAAGAA GAAAGTTCAG 
ATCCCTGTAA GTCACCCAGA TCCTGAGCCG GTGTCTGATA ATGAAGATGA TAGCTACGAC 
GAAGAAGTAC ATGACCCAAG AGCTGGCCGC GGTGCTTTAG CGAACAGAAG GAGCGAGAAG 
AGCTGGGCAA GGGATAGGAG TGCAAGCAGG GAGAGGAGCC TGTCCCCTCG CTCGGACAGG 
CGGTCGGTGG CCTCCAGTCA GCCCGCAAAA CCCACCAAGG TCACACTGGT GAAGTCTCGG 
AAAAATGAAG AATATGGTCT TCGATTGGCC AGCCATATAT TTGTAAAGGA AATTTCACAA 
GATAGTTTGG CAGCAAGAGA TGGTAACATT CAAGAAGGGG ATGTTGTCTT GAAGATAAAT 
GGTACTGTGA CAGAAAATAT GTCATTGACA GATGCAAAAA CACTGATAGA AAGGTCTAAA 
GGCAAGTTAA AAATGGTAGT GCAAAGAGAT GAGCGGGCTA CCTTACTGAA CGTCCCTGAC 
CTTTCGGATA GTATCCATTC TGCTAATGCC TCGGAAAGAG ATGACATTTC AGAAATTCAG 
TCACTAGCAT CAGACCATTC AGGTCGCTCG CATGACAGGC CACCCCGCCG CAGCCAGTCA 
CGATCTCCTG ACCAACGTTC AGAGCCCTCC GATCATTCCA CACAGTCTCC ACAGCAGCCC 
AGCAATGGCA GTCTCCGGAG CAGAGAGCAA GAGCGAATGT CTAAACCTGG GGCCATCTCA 
ACTCCTGTAA AACATGCAGA TGATCATACA CCCAAAGCAG TGGAAGAAGT TACAGTTGAG 
AAAAATGAGA AGCAGACACC CACTCTTCCA GAACCGAAGC CTGTATATGC TCAAGTTGGA 
CAACCAGATG TGGATTTACC CGTCAGCCCT TCTGATGGTG TTCTGCCTAA TTCAACTCAT 
GAAGATGGGA TACTTAGGCC CAGCATGAAA CTGGTAAAAT TCAGAAAAGG AGATAGTGTG 
GGTTTGCGAC TAGCTGGTGG AAATGATGTC GGAATATTTG TAGCTGGCGT TCTAGAAGAT 
AGCCCTGCAG CCAAAGAAGG CTTAGAGGAA GGTGATCAAA TTCTCAGGGT GAACAATGTA 
GATTTCACAA ATATCATAAG AGAAGAGGCT GTCCTTTTCC TCCTTGACCT CCCTAAAGGT 
GAAGAAGTGA CCATCCTGGC TCAGAAGAAG AAGGGTGTTT ATCGCCGCAT TGTAGAATCA 
GATGTAGGAG ATTCATTCTA TATTAGAACG CATTTTGAGT ATGAAAAAGA ATCTCCTTAC 
GGACTTAGTT TTAACAAAGG AGAGGTGTTC CGGGTCGTGG ATACTTTATA CAATGGAAAG 
CTGGGCTCTT GGCTTGCCAT TCGAATTGGC AAAAATCATA AGGAGGTAGA ACGAGGCATC 
ATCCCTAATA AGAACAGAGC TGAGCAGTTA GCCAGTGTAC AGTACACACT TCCAAAGACA 
GCGGGTGGTG ATCGGGCAGA CTTCTGGAGG TTTCGAGGTC TTCGCAGCTC CAAGAGAAAT 
CTTCGAAAAA GCAGAGAGGA CTTGTCAGCT CAGCCAGTTC AAACTAAGTT CCCAGCTTAT 
GAAAGGGTTG TTCTTCGAGA AGCTGGATTC CTAAGACCTG TAACCATCTT TGGACCAATA 
GCTGATGTTG CCAGAGAAAA GTTGGCAAGA GAGGAACCAG ATATCTATCA GATTGCAAAA 
AGTGAACCAC GAGATGCTGG GACTGACCAT CGTAGCTCTG GCATCATTCG CCTTCATACA 
ATAAAGCAAA TCATAGATCA GGATAAACAT GCGTTATTAG ATGTAACGCC AAATGCGGTT 
GATCGTCTTA ATTATGCGCA GTGGTATCCA ATTGTTGTGT TCCTTAACCC TGACTCTAAG 
CAAGGTGTAA AAACAATGAG GATGAGGTTA TGTCCGGAGT CTCGGAAAAG CGCCAGGAAG 
CTATATGAAC GCTCTCATAA GCTTCGCAAA AACAATCACC ACCTTTTCAC AACTACAATT 
AACTTAAACT CAATGAATGA TGGTTGGTAC GGTGCCCTGA AAGAAGCCAT TCAGCAGCAA 
CAGAACCAGC TGGTGTGGGT CTCTGAGGGG AAGGCGGATG GTGCTACAAG TGATGACCTT 
GATTTGCATG ATGATCGTCT GTCCTACCTG TCAGCCCCAG GTAGTGAGTA CTCAATGTAT 
AGCACGGACA GTAGACACAC TTCTGACTAT GAAGACACAG ATACAGAAGG CGGGGCCTAC 
ACTGATCAAG AACTAGATGA AACTCTTAAT GATGAGGTGG GGACTCCCCC GGAGTCTGCC 
ATTACACNGT CCTCTGAGCC TGTAAGAGAG GATTCCTCTG GGATGCATCA TGAAAACCAG 
ACCTATCCTC CTTACTCACC ACAAGCGCAG CCACAAGCTA TTCATAGACT AGACTCCCCT 
GGACTTAAGC CAGCCTCTCA ACAGAAAGCA GAAGCCTCAT CTCCAGTCCC TTACCTTTCG 
CCTGAAACAA ACCCAGCATC GTCAGCCTCT GCAGTTAATC CTAATGTCAG TGTAACTAAT 
GTCAGCCTGG AGGAGCCTGC CCCAGCCCCT CCCGCCTCGC ACGCATCCCA GGCTGGTGGT 
TTAGGAGCAC CAGGTGCTGA GGCAGCTCAC GTAGTGCTCA GAGGTGAAGG ACCACCATTG 
CCGCCGCATG CAGACCCAGC ACAGGTGTAT AGAAAGGAGC CGTATCCTGA AGAAATGATG 
AGACAAAACC ATATTTTAAA ACAACCAGCT CTTGGTCACC CAGGGCAGAG GCCAGATAAA 
GAGCCAAATC TAGCCTATGA ACCCCAACTT CCATATANAG AAAAACAAGC CAGCAGAGAC 
CTTGAACAGC CGTCATACAG GTATGAGGCC TCAAGCTACC CAGACCAGTT TTCTCGGAAC 
TATGACCATC GCCTACGGTT TGAAGATCGA ATCCCTACCT ATGAAGACCA GTGGTCATAT 
TATGATGACA AACAGCCCTA CCAACCTCGG CCTTTTGAGA ATCAGCATCC CCGAGACCTG 
GACTCCAGAC AACATCCNGA GGAGGCTTCA GAACGAGGTT ATNTCCAGCG TTTTGAAGAG 
CCAGCCCCTC TGTCATACGA CAGTAGAACA CGCTATGAGC AGCTGCCTCG AACCTCTACT 
CTACGACATG AAGAGCAGCC AGCCCCTGCA TATGAGGTGC ACAACAGGTA CAGGCCAGAG 
GCACAGCCCT ATTCTTCAAC AGGTCCTAAG TCATCTGAGC CCAAGCAGTA CTTTGACCAG 
TACCCGCGAA GTTATGAGCA AGTACCACCA CCAGGCTTTA CCTCCAAAAC AGGCCATTAC 
GAGCCTCTCC ATGGTGCTGC AGTTGTCCCT CCTCTGATAC CTTCCTCTCA ACATAAGCCA 
GAAGTCCTGC CCTCGGCTAC CAAACCACAG CCTCCACCCC CAACCCTAAC TGAGGAGGAG 
GAGGATCCAG CAATGAAACC ACAGTCCGTG CTCANCAGAG TCAAAATGTT TGAAAACAAA 
AGATCTGCGT CTTTGGAGAA CAAGAAAGAT GTGAATGACA CTGCCAGCTT TAAGCCTCCA 
GAAGTAGCAT CTAAACCTCC AAGTGCTTCT CTTGCTGGCC CTAAACCTGT TCCTCAGAGT 
CAGTTTAGTG AGCANGACAA AACACTCTAC AGGCTCCCAG AGCCTCAGAA ACCTCAAGTG 
AAGCCACCCG ANGATATTGT TCGATCAAAT CATTACGACC CTGAAGAGGA TGAAGAATAT 
TACAGGAAAC AGCTCTCTTA CTTTGANCGA AGAAGTTTTG AGAGCAAGCC TTCTGCACAT 
CTTCCTGCTG GCCATCACTC AGAGCCTGCT AAGCCAGTCC ATTCTCAGAG CCAGCCCAAC 
TTTTCTAGTT ACTCTTCAAA GGGAAAACCC GAAACTGATG CTGTGGATAG ATCATTCAGT 
GAGAAACGTT ATGATCCAGC CCAGGCCACG CCTCCTCCTC CTCCGTTGCC CTCACAGTAC 
AGCCAGCCAG CTCCCCCTCT GTCCAGCTCT TCTCTCCACA TACATTCCAA GGGCGCCCAG 
GGCGAAGGCA ATTCAGTATC ATTGGATTTT CAGAACTCAT ATATGTCCAA ACCAGACCCA 
CCCCCATCTC AGAGTAAACC AGCAACTTTC AGACCACCAA CCCGAGAAGA CCCTCCTCAG 
ACTTTCTATC CCCAGAAANG TTTCCCAGAC AAAGCTCCAG TTAANGGAGC TGAGCAGACT 
CAGAAAACCA TCACTCCAGT GTACAATCGA TTCACACCAA AGCCGTACAC AAGTTCTGCC 
CGGCCGTTTG AACGCAAATT TGAAAGTCCG AAGTTCAACC ATAATCTTCT GCCAAGTGAA 
ACTGTACATA AACCTGAATT GTCTTCAAAA ACTCCCACTT CTCCAAAAAC TCTTATGAAA 
GCTCATAGTT CAACACAGCC TCCAGAGTTT GACAGTGGAG TTGAAACTTT CTCTGTTCAC 
ACAGATNAGC CTAAATATCA AATGAATAAT ATCAGCACCA TGCCTAAAGC TGTCCCTGTG 
AGTCCTTCAG CTGTGGAAGA AGATGAAGAT GAGGATGGTC ATACTGTAGT GGCTACAGCT 
CGTGGCATTT TTAACAGCAA TGGTGGTGTG TTGAGTTCCA TAGAAACCGG TGTTAGTATA 
ATTATCCCAC AAGGAGCCAT TCCTGAAGGA ATTGAACAAG AAATCTATTT CAAAGTCTGC 
AGAGACAATA GCATTCTCCC ACCTTTAGAT AAAGAGAAAG GTGAAACTCT GCTAAGTCCC 
CTAGTGATGT GTGGGCCCCA TGGCCTCAAG TTCCTGAAGC CCGTGGAGCT GCGCTTGCCA 
CACTGTGCGT CCATGACTCC TGACGGTTGG TCTTTTGCTC TAAAATCATC CGACTCCTCG 
TCGGGTGACC CTAAAACCTG GCAAAACAAG TGTCTTCCTG GAGATCCGAA TTACCTTGTT 
GGAGCCAACT GTGTTTCTGT CCTGATTGAC CACTTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_029334976.1
CDS	68..5566
Translation	MKYQKYLTVLQMAIGVTPSNRGSLLPLKRKLWVTPSSENPHGATSNVSQGKPSLRRIKGRLHRSKSLDSIDFCELTSTAMEETAIWEQHTVTLHRAPGFGFGIAISGGRDNPHFQSGETSIVISDVLKGGPAEGQLQENDRVAMVNGVSMDNVEHAFAVQQLRKSGKNAKITIRRKKKVQIPVSHPDPEPVSDNEDDSYDEEVHDPRAGRGALANRRSEKSWARDRSASRERSLSPRSDRRSVASSQPAKPTKVTLVKSRKNEEYGLRLASHIFVKEISQDSLAARDGNIQEGDVVLKINGTVTENMSLTDAKTLIERSKGKLKMVVQRDERATLLNVPDLSDSIHSANASERDDISEIQSLASDHSGRSHDRPPRRSQSRSPDQRSEPSDHSTQSPQQPSNGSLRSREQERMSKPGAISTPVKHADDHTPKAVEEVTVEKNEKQTPTLPEPKPVYAQVGQPDVDLPVSPSDGVLPNSTHEDGILRPSMKLVKFRKGDSVGLRLAGGNDVGIFVAGVLEDSPAAKEGLEEGDQILRVNNVDFTNIIREEAVLFLLDLPKGEEVTILAQKKKGVYRRIVESDVGDSFYIRTHFEYEKESPYGLSFNKGEVFRVVDTLYNGKLGSWLAIRIGKNHKEVERGIIPNKNRAEQLASVQYTLPKTAGGDRADFWRFRGLRSSKRNLRKSREDLSAQPVQTKFPAYERVVLREAGFLRPVTIFGPIADVAREKLAREEPDIYQIAKSEPRDAGTDHRSSGIIRLHTIKQIIDQDKHALLDVTPNAVDRLNYAQWYPIVVFLNPDSKQGVKTMRMRLCPESRKSARKLYERSHKLRKNNHHLFTTTINLNSMNDGWYGALKEAIQQQQNQLVWVSEGKADGATSDDLDLHDDRLSYLSAPGSEYSMYSTDSRHTSDYEDTDTEGGAYTDQELDETLNDEVGTPPESAITnullSSEPVREDSSGMHHENQTYPPYSPQAQPQAIHRLDSPGLKPASQQKAEASSPVPYLSPETNPASSASAVNPNVSVTNVSLEEPAPAPPASHASQAGGLGAPGAEAAHVVLRGEGPPLPPHADPAQVYRKEPYPEEMMRQNHILKQPALGHPGQRPDKEPNLAYEPQLPYnullEKQASRDLEQPSYRYEASSYPDQFSRNYDHRLRFEDRIPTYEDQWSYYDDKQPYQPRPFENQHPRDLDSRQHnullEEASERGYnullQRFEEPAPLSYDSRTRYEQLPRTSTLRHEEQPAPAYEVHNRYRPEAQPYSSTGPKSSEPKQYFDQYPRSYEQVPPPGFTSKTGHYEPLHGAAVVPPLIPSSQHKPEVLPSATKPQPPPPTLTEEEEDPAMKPQSVLnullRVKMFENKRSASLENKKDVNDTASFKPPEVASKPPSASLAGPKPVPQSQFSEnullDKTLYRLPEPQKPQVKPPnullDIVRSNHYDPEEDEEYYRKQLSYFnullRRSFESKPSAHLPAGHHSEPAKPVHSQSQPNFSSYSSKGKPETDAVDRSFSEKRYDPAQATPPPPPLPSQYSQPAPPLSSSSLHIHSKGAQGEGNSVSLDFQNSYMSKPDPPPSQSKPATFRPPTREDPPQTFYPQKnullFPDKAPVnullGAEQTQKTITPVYNRFTPKPYTSSARPFERKFESPKFNHNLLPSETVHKPELSSKTPTSPKTLMKAHSSTQPPEFDSGVETFSVHTDnullPKYQMNNISTMPKAVPVSPSAVEEDEDEDGHTVVATARGIFNSNGGVLSSIETGVSIIIPQGAIPEGIEQEIYFKVCRDNSILPPLDKEKGETLLSPLVMCGPHGLKFLKPVELRLPHCASMTPDGWSFALKSSDSSSGDPKTWQNKCLPGDPNYLVGANCVSVLIDHF

Target ORF information:

RefSeq Version	XM_029479116.1
Organism	Mus caroli(Ryukyu mouse)
Definition	Mus caroli tight junction protein 1 (Tjp1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_029479116.1

ORF Insert Sequence:

ATGAAGTACC AGAAATACCT GACGGTGCTG CAGATGGCCA TCGGCGTCAC CCCGTCCAAC 
CGCGGCAGCC TCCTGCCGCT CAAGAGGAAG CTGTGGGTAA CTCCATCCTC TGAAAACCCG 
CATGGTGCTA CTTCCAATGT CAGCCAAGGA AAACCTTCTC TAAGACGAAT TAAAGGGAGA 
CTACATCGAA GCAAAAGCCT TGACAGTATC GACTTCTGTG AACTCACTAG CACAGCAATG 
GAGGAAACGG CTATATGGGA ACAGCACACA GTGACGCTTC ACAGGGCTCC TGGGTTTGGA 
TTTGGAATTG CAATCTCTGG TGGAAGAGAT AATCCTCATT TTCAGAGTGG GGAAACCTCC 
ATAGTGATTT CTGATGTGCT AAAAGGAGGG CCAGCTGAAG GACAGCTACA GGAAAATGAC 
CGAGTTGCAA TGGTTAATGG AGTTTCAATG GATAACGTTG AGCATGCTTT TGCTGTTCAG 
CAGCTAAGGA AAAGTGGGAA AAACGCAAAA ATTACTATCC GAAGGAAGAA GAAAGTTCAG 
ATCCCTGTAA GTCACCCAGA TCCTGAGCCG GTGTCTGATA ATGAAGATGA TAGCTACGAC 
GAAGAAGTAC ATGACCCAAG AGCTGGCCGC GGTGCTTTAG CGAACAGAAG GAGCGAGAAG 
AGCTGGGCAA GGGATAGGAG TGCAAGCAGG GAGAGGAGCC TGTCCCCTCG CTCGGACAGG 
CGGTCGGTGG CCTCCAGTCA GCCCGCAAAA CCCACCAAGG TCACACTGGT GAAGTCTCGG 
AAAAATGAAG AATATGGTCT TCGATTGGCC AGCCATATAT TTGTAAAGGA AATTTCACAA 
GATAGTTTGG CAGCAAGAGA TGGTAACATT CAAGAAGGGG ATGTTGTCTT GAAGATAAAT 
GGTACTGTGA CAGAAAATAT GTCATTGACA GATGCAAAAA CACTGATAGA AAGGTCTAAA 
GGCAAGTTAA AAATGGTAGT GCAAAGAGAT GAGCGGGCTA CCTTACTGAA CGTCCCTGAC 
CTTTCGGATA GTATCCATTC TGCTAATGCC TCGGAAAGAG ATGACATTTC AGAAATTCAG 
TCACTAGCAT CAGACCATTC AGGTCGCTCG CATGACAGGC CACCCCGCCG CAGCCAGTCA 
CGATCTCCTG ACCAACGTTC AGAGCCCTCC GATCATTCCA CACAGTCTCC ACAGCAGCCC 
AGCAATGGCA GTCTCCGGAG CAGAGAGCAA GAGCGAATGT CTAAACCTGG GGCCATCTCA 
ACTCCTGTAA AACATGCAGA TGATCATACA CCCAAAGCAG TGGAAGAAGT TACAGTTGAG 
AAAAATGAGA AGCAGACACC CACTCTTCCA GAACCGAAGC CTGTATATGC TCAAGTTGGA 
CAACCAGATG TGGATTTACC CGTCAGCCCT TCTGATGGTG TTCTGCCTAA TTCAACTCAT 
GAAGATGGGA TACTTAGGCC CAGCATGAAA CTGGTAAAAT TCAGAAAAGG AGATAGTGTG 
GGTTTGCGAC TAGCTGGTGG AAATGATGTC GGAATATTTG TAGCTGGCGT TCTAGAAGAT 
AGCCCTGCAG CCAAAGAAGG CTTAGAGGAA GGTGATCAAA TTCTCAGGGT GAACAATGTA 
GATTTCACAA ATATCATAAG AGAAGAGGCT GTCCTTTTCC TCCTTGACCT CCCTAAAGGT 
GAAGAAGTGA CCATCCTGGC TCAGAAGAAG AAGGGTGTTT ATCGCCGCAT TGTAGAATCA 
GATGTAGGAG ATTCATTCTA TATTAGAACG CATTTTGAGT ATGAAAAAGA ATCTCCTTAC 
GGACTTAGTT TTAACAAAGG AGAGGTGTTC CGGGTCGTGG ATACTTTATA CAATGGAAAG 
CTGGGCTCTT GGCTTGCCAT TCGAATTGGC AAAAATCATA AGGAGGTAGA ACGAGGCATC 
ATCCCTAATA AGAACAGAGC TGAGCAGTTA GCCAGTGTAC AGTACACACT TCCAAAGACA 
GCGGGTGGTG ATCGGGCAGA CTTCTGGAGG TTTCGAGGTC TTCGCAGCTC CAAGAGAAAT 
CTTCGAAAAA GCAGAGAGGA CTTGTCAGCT CAGCCAGTTC AAACTAAGTT CCCAGCTTAT 
GAAAGGGTTG TTCTTCGAGA AGCTGGATTC CTAAGACCTG TAACCATCTT TGGACCAATA 
GCTGATGTTG CCAGAGAAAA GTTGGCAAGA GAGGAACCAG ATATCTATCA GATTGCAAAA 
AGTGAACCAC GAGATGCTGG GACTGACCAT CGTAGCTCTG GCATCATTCG CCTTCATACA 
ATAAAGCAAA TCATAGATCA GGATAAACAT GCGTTATTAG ATGTAACGCC AAATGCGGTT 
GATCGTCTTA ATTATGCGCA GTGGTATCCA ATTGTTGTGT TCCTTAACCC TGACTCTAAG 
CAAGGTGTAA AAACAATGAG GATGAGGTTA TGTCCGGAGT CTCGGAAAAG CGCCAGGAAG 
CTATATGAAC GCTCTCATAA GCTTCGCAAA AACAATCACC ACCTTTTCAC AACTACAATT 
AACTTAAACT CAATGAATGA TGGTTGGTAC GGTGCCCTGA AAGAAGCCAT TCAGCAGCAA 
CAGAACCAGC TGGTGTGGGT CTCTGAGGGG AAGGCGGATG GTGCTACAAG TGATGACCTT 
GATTTGCATG ATGATCGTCT GTCCTACCTG TCAGCCCCAG GTAGTGAGTA CTCAATGTAT 
AGCACGGACA GTAGACACAC TTCTGACTAT GAAGACACAG ATACAGAAGG CGGGGCCTAC 
ACTGATCAAG AACTAGATGA AACTCTTAAT GATGAGGTGG GGACTCCCCC GGAGTCTGCC 
ATTACACNGT CCTCTGAGCC TGTAAGAGAG GATTCCTCTG GGATGCATCA TGAAAACCAG 
ACCTATCCTC CTTACTCACC ACAAGCGCAG CCACAAGCTA TTCATAGACT AGACTCCCCT 
GGACTTAAGC CAGCCTCTCA ACAGAAAGCA GAAGCCTCAT CTCCAGTCCC TTACCTTTCG 
CCTGAAACAA ACCCAGCATC GTCAGCCTCT GCAGTTAATC CTAATGTCAG TGTAACTAAT 
GTCAGCCTGG AGGAGCCTGC CCCAGCCCCT CCCGCCTCGC ACGCATCCCA GGCTGGTGGT 
TTAGGAGCAC CAGGTGCTGA GGCAGCTCAC GTAGTGCTCA GAGGTGAAGG ACCACCATTG 
CCGCCGCATG CAGACCCAGC ACAGGTGTAT AGAAAGGAGC CGTATCCTGA AGAAATGATG 
AGACAAAACC ATATTTTAAA ACAACCAGCT CTTGGTCACC CAGGGCAGAG GCCAGATAAA 
GAGCCAAATC TAGCCTATGA ACCCCAACTT CCATATANAG AAAAACAAGC CAGCAGAGAC 
CTTGAACAGC CGTCATACAG GTATGAGGCC TCAAGCTACC CAGACCAGTT TTCTCGGAAC 
TATGACCATC GCCTACGGTT TGAAGATCGA ATCCCTACCT ATGAAGACCA GTGGTCATAT 
TATGATGACA AACAGCCCTA CCAACCTCGG CCTTTTGAGA ATCAGCATCC CCGAGACCTG 
GACTCCAGAC AACATCCNGA GGAGGCTTCA GAACGAGGTT ATNTCCAGCG TTTTGAAGAG 
CCAGCCCCTC TGTCATACGA CAGTAGAACA CGCTATGAGC AGCTGCCTCG AACCTCTACT 
CTACGACATG AAGAGCAGCC AGCCCCTGCA TATGAGGTGC ACAACAGGTA CAGGCCAGAG 
GCACAGCCCT ATTCTTCAAC AGGTCCTAAG TCATCTGAGC CCAAGCAGTA CTTTGACCAG 
TACCCGCGAA GTTATGAGCA AGTACCACCA CCAGGCTTTA CCTCCAAAAC AGGCCATTAC 
GAGCCTCTCC ATGGTGCTGC AGTTGTCCCT CCTCTGATAC CTTCCTCTCA ACATAAGCCA 
GAAGTCCTGC CCTCGGCTAC CAAACCACAG CCTCCACCCC CAACCCTAAC TGAGGAGGAG 
GAGGATCCAG CAATGAAACC ACAGTCCGTG CTCANCAGAG TCAAAATGTT TGAAAACAAA 
AGATCTGCGT CTTTGGAGAA CAAGAAAGAT GTGAATGACA CTGCCAGCTT TAAGCCTCCA 
GAAGTAGCAT CTAAACCTCC AAGTGCTTCT CTTGCTGGCC CTAAACCTGT TCCTCAGAGT 
CAGTTTAGTG AGCANGACAA AACACTCTAC AGGCTCCCAG AGCCTCAGAA ACCTCAAGTG 
AAGCCACCCG ANGATATTGT TCGATCAAAT CATTACGACC CTGAAGAGGA TGAAGAATAT 
TACAGGAAAC AGCTCTCTTA CTTTGANCGA AGAAGTTTTG AGAGCAAGCC TTCTGCACAT 
CTTCCTGCTG GCCATCACTC AGAGCCTGCT AAGCCAGTCC ATTCTCAGAG CCAGCCCAAC 
TTTTCTAGTT ACTCTTCAAA GGGAAAACCC GAAACTGATG CTGTGGATAG ATCATTCAGT 
GAGAAACGTT ATGATCCAGC CCAGGCCACG CCTCCTCCTC CTCCGTTGCC CTCACAGTAC 
AGCCAGCCAG CTCCCCCTCT GTCCAGCTCT TCTCTCCACA TACATTCCAA GGGCGCCCAG 
GGCGAAGGCA ATTCAGTATC ATTGGATTTT CAGAACTCAT ATATGTCCAA ACCAGACCCA 
CCCCCATCTC AGAGTAAACC AGCAACTTTC AGACCACCAA CCCGAGAAGA CCCTCCTCAG 
ACTTTCTATC CCCAGAAANG TTTCCCAGAC AAAGCTCCAG TTAANGGAGC TGAGCAGACT 
CAGAAAACCA TCACTCCAGT GTACAATCGA TTCACACCAA AGCCGTACAC AAGTTCTGCC 
CGGCCGTTTG AACGCAAATT TGAAAGTCCG AAGTTCAACC ATAATCTTCT GCCAAGTGAA 
ACTGTACATA AACCTGAATT GTCTTCAAAA ACTCCCACTT CTCCAAAAAC TCTTATGAAA 
GCTCATAGTT CAACACAGCC TCCAGAGTTT GACAGTGGAG TTGAAACTTT CTCTGTTCAC 
ACAGATNAGC CTAAATATCA AATGAATAAT ATCAGCACCA TGCCTAAAGC TGTCCCTGTG 
AGTCCTTCAG CTGTGGAAGA AGATGAAGAT GAGGATGGTC ATACTGTAGT GGCTACAGCT 
CGTGGCATTT TTAACAGCAA TGGTGGTGTG TTGAGTTCCA TAGAAACCGG TGTTAGTATA 
ATTATCCCAC AAGGAGCCAT TCCTGAAGGA ATTGAACAAG AAATCTATTT CAAAGTCTGC 
AGAGACAATA GCATTCTCCC ACCTTTAGAT AAAGAGAAAG GTGAAACTCT GCTAAGTCCC 
CTAGTGATGT GTGGGCCCCA TGGCCTCAAG TTCCTGAAGC CCGTGGAGCT GCGCTTGCCA 
CACTGTGCGT CCATGACTCC TGACGGTTGG TCTTTTGCTC TAAAATCATC CGACTCCTCG 
TCGGGTGACC CTAAAACCTG GCAAAACAAG TGTCTTCCTG GAGATCCGAA TTACCTTGTT 
GGAGCCAACT GTGTTTCTGT CCTGATTGAC CACTTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Tjp1 ( XM_029479117.1 ) cDNA ORF clone, Mus caroli(Ryukyu mouse) -> XP_029334977.1 Mus caroli tight junction protein 1 (Tjp1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OMv74614
Clone ID Related Accession (Same CDS sequence)	XM_029479117.1
Accession Version	XM_029479117.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5298bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-06-09
Organism	Mus caroli(Ryukyu mouse)
Product	tight junction protein ZO-1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_034576.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus caroli Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCGCCA GGGCCGCGGC CGCTAAGAGC ACAGCAATGG AGGAAACGGC TATATGGGAA 
CAGCACACAG TGACGCTTCA CAGGGCTCCT GGGTTTGGAT TTGGAATTGC AATCTCTGGT 
GGAAGAGATA ATCCTCATTT TCAGAGTGGG GAAACCTCCA TAGTGATTTC TGATGTGCTA 
AAAGGAGGGC CAGCTGAAGG ACAGCTACAG GAAAATGACC GAGTTGCAAT GGTTAATGGA 
GTTTCAATGG ATAACGTTGA GCATGCTTTT GCTGTTCAGC AGCTAAGGAA AAGTGGGAAA 
AACGCAAAAA TTACTATCCG AAGGAAGAAG AAAGTTCAGA TCCCTGTAAG TCACCCAGAT 
CCTGAGCCGG TGTCTGATAA TGAAGATGAT AGCTACGACG AAGAAGTACA TGACCCAAGA 
GCTGGCCGCG GTGCTTTAGC GAACAGAAGG AGCGAGAAGA GCTGGGCAAG GGATAGGAGT 
GCAAGCAGGG AGAGGAGCCT GTCCCCTCGC TCGGACAGGC GGTCGGTGGC CTCCAGTCAG 
CCCGCAAAAC CCACCAAGGT CACACTGGTG AAGTCTCGGA AAAATGAAGA ATATGGTCTT 
CGATTGGCCA GCCATATATT TGTAAAGGAA ATTTCACAAG ATAGTTTGGC AGCAAGAGAT 
GGTAACATTC AAGAAGGGGA TGTTGTCTTG AAGATAAATG GTACTGTGAC AGAAAATATG 
TCATTGACAG ATGCAAAAAC ACTGATAGAA AGGTCTAAAG GCAAGTTAAA AATGGTAGTG 
CAAAGAGATG AGCGGGCTAC CTTACTGAAC GTCCCTGACC TTTCGGATAG TATCCATTCT 
GCTAATGCCT CGGAAAGAGA TGACATTTCA GAAATTCAGT CACTAGCATC AGACCATTCA 
GGTCGCTCGC ATGACAGGCC ACCCCGCCGC AGCCAGTCAC GATCTCCTGA CCAACGTTCA 
GAGCCCTCCG ATCATTCCAC ACAGTCTCCA CAGCAGCCCA GCAATGGCAG TCTCCGGAGC 
AGAGAGCAAG AGCGAATGTC TAAACCTGGG GCCATCTCAA CTCCTGTAAA ACATGCAGAT 
GATCATACAC CCAAAGCAGT GGAAGAAGTT ACAGTTGAGA AAAATGAGAA GCAGACACCC 
ACTCTTCCAG AACCGAAGCC TGTATATGCT CAAGTTGGAC AACCAGATGT GGATTTACCC 
GTCAGCCCTT CTGATGGTGT TCTGCCTAAT TCAACTCATG AAGATGGGAT ACTTAGGCCC 
AGCATGAAAC TGGTAAAATT CAGAAAAGGA GATAGTGTGG GTTTGCGACT AGCTGGTGGA 
AATGATGTCG GAATATTTGT AGCTGGCGTT CTAGAAGATA GCCCTGCAGC CAAAGAAGGC 
TTAGAGGAAG GTGATCAAAT TCTCAGGGTG AACAATGTAG ATTTCACAAA TATCATAAGA 
GAAGAGGCTG TCCTTTTCCT CCTTGACCTC CCTAAAGGTG AAGAAGTGAC CATCCTGGCT 
CAGAAGAAGA AGGGTGTTTA TCGCCGCATT GTAGAATCAG ATGTAGGAGA TTCATTCTAT 
ATTAGAACGC ATTTTGAGTA TGAAAAAGAA TCTCCTTACG GACTTAGTTT TAACAAAGGA 
GAGGTGTTCC GGGTCGTGGA TACTTTATAC AATGGAAAGC TGGGCTCTTG GCTTGCCATT 
CGAATTGGCA AAAATCATAA GGAGGTAGAA CGAGGCATCA TCCCTAATAA GAACAGAGCT 
GAGCAGTTAG CCAGTGTACA GTACACACTT CCAAAGACAG CGGGTGGTGA TCGGGCAGAC 
TTCTGGAGGT TTCGAGGTCT TCGCAGCTCC AAGAGAAATC TTCGAAAAAG CAGAGAGGAC 
TTGTCAGCTC AGCCAGTTCA AACTAAGTTC CCAGCTTATG AAAGGGTTGT TCTTCGAGAA 
GCTGGATTCC TAAGACCTGT AACCATCTTT GGACCAATAG CTGATGTTGC CAGAGAAAAG 
TTGGCAAGAG AGGAACCAGA TATCTATCAG ATTGCAAAAA GTGAACCACG AGATGCTGGG 
ACTGACCATC GTAGCTCTGG CATCATTCGC CTTCATACAA TAAAGCAAAT CATAGATCAG 
GATAAACATG CGTTATTAGA TGTAACGCCA AATGCGGTTG ATCGTCTTAA TTATGCGCAG 
TGGTATCCAA TTGTTGTGTT CCTTAACCCT GACTCTAAGC AAGGTGTAAA AACAATGAGG 
ATGAGGTTAT GTCCGGAGTC TCGGAAAAGC GCCAGGAAGC TATATGAACG CTCTCATAAG 
CTTCGCAAAA ACAATCACCA CCTTTTCACA ACTACAATTA ACTTAAACTC AATGAATGAT 
GGTTGGTACG GTGCCCTGAA AGAAGCCATT CAGCAGCAAC AGAACCAGCT GGTGTGGGTC 
TCTGAGGGGA AGGCGGATGG TGCTACAAGT GATGACCTTG ATTTGCATGA TGATCGTCTG 
TCCTACCTGT CAGCCCCAGG TAGTGAGTAC TCAATGTATA GCACGGACAG TAGACACACT 
TCTGACTATG AAGACACAGA TACAGAAGGC GGGGCCTACA CTGATCAAGA ACTAGATGAA 
ACTCTTAATG ATGAGGTGGG GACTCCCCCG GAGTCTGCCA TTACACNGTC CTCTGAGCCT 
GTAAGAGAGG ATTCCTCTGG GATGCATCAT GAAAACCAGA CCTATCCTCC TTACTCACCA 
CAAGCGCAGC CACAAGCTAT TCATAGACTA GACTCCCCTG GACTTAAGCC AGCCTCTCAA 
CAGAAAGCAG AAGCCTCATC TCCAGTCCCT TACCTTTCGC CTGAAACAAA CCCAGCATCG 
TCAGCCTCTG CAGTTAATCC TAATGTCAGT GTAACTAATG TCAGCCTGGA GGAGCCTGCC 
CCAGCCCCTC CCGCCTCGCA CGCATCCCAG GCTGGTGGTT TAGGAGCACC AGGTGCTGAG 
GCAGCTCACG TAGTGCTCAG AGGTGAAGGA CCACCATTGC CGCCGCATGC AGACCCAGCA 
CAGGTGTATA GAAAGGAGCC GTATCCTGAA GAAATGATGA GACAAAACCA TATTTTAAAA 
CAACCAGCTC TTGGTCACCC AGGGCAGAGG CCAGATAAAG AGCCAAATCT AGCCTATGAA 
CCCCAACTTC CATATANAGA AAAACAAGCC AGCAGAGACC TTGAACAGCC GTCATACAGG 
TATGAGGCCT CAAGCTACCC AGACCAGTTT TCTCGGAACT ATGACCATCG CCTACGGTTT 
GAAGATCGAA TCCCTACCTA TGAAGACCAG TGGTCATATT ATGATGACAA ACAGCCCTAC 
CAACCTCGGC CTTTTGAGAA TCAGCATCCC CGAGACCTGG ACTCCAGACA ACATCCNGAG 
GAGGCTTCAG AACGAGGTTA TNTCCAGCGT TTTGAAGAGC CAGCCCCTCT GTCATACGAC 
AGTAGAACAC GCTATGAGCA GCTGCCTCGA ACCTCTACTC TACGACATGA AGAGCAGCCA 
GCCCCTGCAT ATGAGGTGCA CAACAGGTAC AGGCCAGAGG CACAGCCCTA TTCTTCAACA 
GGTCCTAAGT CATCTGAGCC CAAGCAGTAC TTTGACCAGT ACCCGCGAAG TTATGAGCAA 
GTACCACCAC CAGGCTTTAC CTCCAAAACA GGCCATTACG AGCCTCTCCA TGGTGCTGCA 
GTTGTCCCTC CTCTGATACC TTCCTCTCAA CATAAGCCAG AAGTCCTGCC CTCGGCTACC 
AAACCACAGC CTCCACCCCC AACCCTAACT GAGGAGGAGG AGGATCCAGC AATGAAACCA 
CAGTCCGTGC TCANCAGAGT CAAAATGTTT GAAAACAAAA GATCTGCGTC TTTGGAGAAC 
AAGAAAGATG TGAATGACAC TGCCAGCTTT AAGCCTCCAG AAGTAGCATC TAAACCTCCA 
AGTGCTTCTC TTGCTGGCCC TAAACCTGTT CCTCAGAGTC AGTTTAGTGA GCANGACAAA 
ACACTCTACA GGCTCCCAGA GCCTCAGAAA CCTCAAGTGA AGCCACCCGA NGATATTGTT 
CGATCAAATC ATTACGACCC TGAAGAGGAT GAAGAATATT ACAGGAAACA GCTCTCTTAC 
TTTGANCGAA GAAGTTTTGA GAGCAAGCCT TCTGCACATC TTCCTGCTGG CCATCACTCA 
GAGCCTGCTA AGCCAGTCCA TTCTCAGAGC CAGCCCAACT TTTCTAGTTA CTCTTCAAAG 
GGAAAACCCG AAACTGATGC TGTGGATAGA TCATTCAGTG AGAAACGTTA TGATCCAGCC 
CAGGCCACGC CTCCTCCTCC TCCGTTGCCC TCACAGTACA GCCAGCCAGC TCCCCCTCTG 
TCCAGCTCTT CTCTCCACAT ACATTCCAAG GGCGCCCAGG GCGAAGGCAA TTCAGTATCA 
TTGGATTTTC AGAACTCATA TATGTCCAAA CCAGACCCAC CCCCATCTCA GAGTAAACCA 
GCAACTTTCA GACCACCAAC CCGAGAAGAC CCTCCTCAGA CTTTCTATCC CCAGAAANGT 
TTCCCAGACA AAGCTCCAGT TAANGGAGCT GAGCAGACTC AGAAAACCAT CACTCCAGTG 
TACAATCGAT TCACACCAAA GCCGTACACA AGTTCTGCCC GGCCGTTTGA ACGCAAATTT 
GAAAGTCCGA AGTTCAACCA TAATCTTCTG CCAAGTGAAA CTGTACATAA ACCTGAATTG 
TCTTCAAAAA CTCCCACTTC TCCAAAAACT CTTATGAAAG CTCATAGTTC AACACAGCCT 
CCAGAGTTTG ACAGTGGAGT TGAAACTTTC TCTGTTCACA CAGATNAGCC TAAATATCAA 
ATGAATAATA TCAGCACCAT GCCTAAAGCT GTCCCTGTGA GTCCTTCAGC TGTGGAAGAA 
GATGAAGATG AGGATGGTCA TACTGTAGTG GCTACAGCTC GTGGCATTTT TAACAGCAAT 
GGTGGTGTGT TGAGTTCCAT AGAAACCGGT GTTAGTATAA TTATCCCACA AGGAGCCATT 
CCTGAAGGAA TTGAACAAGA AATCTATTTC AAAGTCTGCA GAGACAATAG CATTCTCCCA 
CCTTTAGATA AAGAGAAAGG TGAAACTCTG CTAAGTCCCC TAGTGATGTG TGGGCCCCAT 
GGCCTCAAGT TCCTGAAGCC CGTGGAGCTG CGCTTGCCAC ACTGTGCGTC CATGACTCCT 
GACGGTTGGT CTTTTGCTCT AAAATCATCC GACTCCTCGT CGGGTGACCC TAAAACCTGG 
CAAAACAAGT GTCTTCCTGG AGATCCGAAT TACCTTGTTG GAGCCAACTG TGTTTCTGTC 
CTGATTGACC ACTTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_029334977.1
CDS	420..5717
Translation	MSARAAAAKSTAMEETAIWEQHTVTLHRAPGFGFGIAISGGRDNPHFQSGETSIVISDVLKGGPAEGQLQENDRVAMVNGVSMDNVEHAFAVQQLRKSGKNAKITIRRKKKVQIPVSHPDPEPVSDNEDDSYDEEVHDPRAGRGALANRRSEKSWARDRSASRERSLSPRSDRRSVASSQPAKPTKVTLVKSRKNEEYGLRLASHIFVKEISQDSLAARDGNIQEGDVVLKINGTVTENMSLTDAKTLIERSKGKLKMVVQRDERATLLNVPDLSDSIHSANASERDDISEIQSLASDHSGRSHDRPPRRSQSRSPDQRSEPSDHSTQSPQQPSNGSLRSREQERMSKPGAISTPVKHADDHTPKAVEEVTVEKNEKQTPTLPEPKPVYAQVGQPDVDLPVSPSDGVLPNSTHEDGILRPSMKLVKFRKGDSVGLRLAGGNDVGIFVAGVLEDSPAAKEGLEEGDQILRVNNVDFTNIIREEAVLFLLDLPKGEEVTILAQKKKGVYRRIVESDVGDSFYIRTHFEYEKESPYGLSFNKGEVFRVVDTLYNGKLGSWLAIRIGKNHKEVERGIIPNKNRAEQLASVQYTLPKTAGGDRADFWRFRGLRSSKRNLRKSREDLSAQPVQTKFPAYERVVLREAGFLRPVTIFGPIADVAREKLAREEPDIYQIAKSEPRDAGTDHRSSGIIRLHTIKQIIDQDKHALLDVTPNAVDRLNYAQWYPIVVFLNPDSKQGVKTMRMRLCPESRKSARKLYERSHKLRKNNHHLFTTTINLNSMNDGWYGALKEAIQQQQNQLVWVSEGKADGATSDDLDLHDDRLSYLSAPGSEYSMYSTDSRHTSDYEDTDTEGGAYTDQELDETLNDEVGTPPESAITnullSSEPVREDSSGMHHENQTYPPYSPQAQPQAIHRLDSPGLKPASQQKAEASSPVPYLSPETNPASSASAVNPNVSVTNVSLEEPAPAPPASHASQAGGLGAPGAEAAHVVLRGEGPPLPPHADPAQVYRKEPYPEEMMRQNHILKQPALGHPGQRPDKEPNLAYEPQLPYnullEKQASRDLEQPSYRYEASSYPDQFSRNYDHRLRFEDRIPTYEDQWSYYDDKQPYQPRPFENQHPRDLDSRQHnullEEASERGYnullQRFEEPAPLSYDSRTRYEQLPRTSTLRHEEQPAPAYEVHNRYRPEAQPYSSTGPKSSEPKQYFDQYPRSYEQVPPPGFTSKTGHYEPLHGAAVVPPLIPSSQHKPEVLPSATKPQPPPPTLTEEEEDPAMKPQSVLnullRVKMFENKRSASLENKKDVNDTASFKPPEVASKPPSASLAGPKPVPQSQFSEnullDKTLYRLPEPQKPQVKPPnullDIVRSNHYDPEEDEEYYRKQLSYFnullRRSFESKPSAHLPAGHHSEPAKPVHSQSQPNFSSYSSKGKPETDAVDRSFSEKRYDPAQATPPPPPLPSQYSQPAPPLSSSSLHIHSKGAQGEGNSVSLDFQNSYMSKPDPPPSQSKPATFRPPTREDPPQTFYPQKnullFPDKAPVnullGAEQTQKTITPVYNRFTPKPYTSSARPFERKFESPKFNHNLLPSETVHKPELSSKTPTSPKTLMKAHSSTQPPEFDSGVETFSVHTDnullPKYQMNNISTMPKAVPVSPSAVEEDEDEDGHTVVATARGIFNSNGGVLSSIETGVSIIIPQGAIPEGIEQEIYFKVCRDNSILPPLDKEKGETLLSPLVMCGPHGLKFLKPVELRLPHCASMTPDGWSFALKSSDSSSGDPKTWQNKCLPGDPNYLVGANCVSVLIDHF

Target ORF information:

RefSeq Version	XM_029479117.1
Organism	Mus caroli(Ryukyu mouse)
Definition	Mus caroli tight junction protein 1 (Tjp1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_029479117.1

ORF Insert Sequence:

ATGTCCGCCA GGGCCGCGGC CGCTAAGAGC ACAGCAATGG AGGAAACGGC TATATGGGAA 
CAGCACACAG TGACGCTTCA CAGGGCTCCT GGGTTTGGAT TTGGAATTGC AATCTCTGGT 
GGAAGAGATA ATCCTCATTT TCAGAGTGGG GAAACCTCCA TAGTGATTTC TGATGTGCTA 
AAAGGAGGGC CAGCTGAAGG ACAGCTACAG GAAAATGACC GAGTTGCAAT GGTTAATGGA 
GTTTCAATGG ATAACGTTGA GCATGCTTTT GCTGTTCAGC AGCTAAGGAA AAGTGGGAAA 
AACGCAAAAA TTACTATCCG AAGGAAGAAG AAAGTTCAGA TCCCTGTAAG TCACCCAGAT 
CCTGAGCCGG TGTCTGATAA TGAAGATGAT AGCTACGACG AAGAAGTACA TGACCCAAGA 
GCTGGCCGCG GTGCTTTAGC GAACAGAAGG AGCGAGAAGA GCTGGGCAAG GGATAGGAGT 
GCAAGCAGGG AGAGGAGCCT GTCCCCTCGC TCGGACAGGC GGTCGGTGGC CTCCAGTCAG 
CCCGCAAAAC CCACCAAGGT CACACTGGTG AAGTCTCGGA AAAATGAAGA ATATGGTCTT 
CGATTGGCCA GCCATATATT TGTAAAGGAA ATTTCACAAG ATAGTTTGGC AGCAAGAGAT 
GGTAACATTC AAGAAGGGGA TGTTGTCTTG AAGATAAATG GTACTGTGAC AGAAAATATG 
TCATTGACAG ATGCAAAAAC ACTGATAGAA AGGTCTAAAG GCAAGTTAAA AATGGTAGTG 
CAAAGAGATG AGCGGGCTAC CTTACTGAAC GTCCCTGACC TTTCGGATAG TATCCATTCT 
GCTAATGCCT CGGAAAGAGA TGACATTTCA GAAATTCAGT CACTAGCATC AGACCATTCA 
GGTCGCTCGC ATGACAGGCC ACCCCGCCGC AGCCAGTCAC GATCTCCTGA CCAACGTTCA 
GAGCCCTCCG ATCATTCCAC ACAGTCTCCA CAGCAGCCCA GCAATGGCAG TCTCCGGAGC 
AGAGAGCAAG AGCGAATGTC TAAACCTGGG GCCATCTCAA CTCCTGTAAA ACATGCAGAT 
GATCATACAC CCAAAGCAGT GGAAGAAGTT ACAGTTGAGA AAAATGAGAA GCAGACACCC 
ACTCTTCCAG AACCGAAGCC TGTATATGCT CAAGTTGGAC AACCAGATGT GGATTTACCC 
GTCAGCCCTT CTGATGGTGT TCTGCCTAAT TCAACTCATG AAGATGGGAT ACTTAGGCCC 
AGCATGAAAC TGGTAAAATT CAGAAAAGGA GATAGTGTGG GTTTGCGACT AGCTGGTGGA 
AATGATGTCG GAATATTTGT AGCTGGCGTT CTAGAAGATA GCCCTGCAGC CAAAGAAGGC 
TTAGAGGAAG GTGATCAAAT TCTCAGGGTG AACAATGTAG ATTTCACAAA TATCATAAGA 
GAAGAGGCTG TCCTTTTCCT CCTTGACCTC CCTAAAGGTG AAGAAGTGAC CATCCTGGCT 
CAGAAGAAGA AGGGTGTTTA TCGCCGCATT GTAGAATCAG ATGTAGGAGA TTCATTCTAT 
ATTAGAACGC ATTTTGAGTA TGAAAAAGAA TCTCCTTACG GACTTAGTTT TAACAAAGGA 
GAGGTGTTCC GGGTCGTGGA TACTTTATAC AATGGAAAGC TGGGCTCTTG GCTTGCCATT 
CGAATTGGCA AAAATCATAA GGAGGTAGAA CGAGGCATCA TCCCTAATAA GAACAGAGCT 
GAGCAGTTAG CCAGTGTACA GTACACACTT CCAAAGACAG CGGGTGGTGA TCGGGCAGAC 
TTCTGGAGGT TTCGAGGTCT TCGCAGCTCC AAGAGAAATC TTCGAAAAAG CAGAGAGGAC 
TTGTCAGCTC AGCCAGTTCA AACTAAGTTC CCAGCTTATG AAAGGGTTGT TCTTCGAGAA 
GCTGGATTCC TAAGACCTGT AACCATCTTT GGACCAATAG CTGATGTTGC CAGAGAAAAG 
TTGGCAAGAG AGGAACCAGA TATCTATCAG ATTGCAAAAA GTGAACCACG AGATGCTGGG 
ACTGACCATC GTAGCTCTGG CATCATTCGC CTTCATACAA TAAAGCAAAT CATAGATCAG 
GATAAACATG CGTTATTAGA TGTAACGCCA AATGCGGTTG ATCGTCTTAA TTATGCGCAG 
TGGTATCCAA TTGTTGTGTT CCTTAACCCT GACTCTAAGC AAGGTGTAAA AACAATGAGG 
ATGAGGTTAT GTCCGGAGTC TCGGAAAAGC GCCAGGAAGC TATATGAACG CTCTCATAAG 
CTTCGCAAAA ACAATCACCA CCTTTTCACA ACTACAATTA ACTTAAACTC AATGAATGAT 
GGTTGGTACG GTGCCCTGAA AGAAGCCATT CAGCAGCAAC AGAACCAGCT GGTGTGGGTC 
TCTGAGGGGA AGGCGGATGG TGCTACAAGT GATGACCTTG ATTTGCATGA TGATCGTCTG 
TCCTACCTGT CAGCCCCAGG TAGTGAGTAC TCAATGTATA GCACGGACAG TAGACACACT 
TCTGACTATG AAGACACAGA TACAGAAGGC GGGGCCTACA CTGATCAAGA ACTAGATGAA 
ACTCTTAATG ATGAGGTGGG GACTCCCCCG GAGTCTGCCA TTACACNGTC CTCTGAGCCT 
GTAAGAGAGG ATTCCTCTGG GATGCATCAT GAAAACCAGA CCTATCCTCC TTACTCACCA 
CAAGCGCAGC CACAAGCTAT TCATAGACTA GACTCCCCTG GACTTAAGCC AGCCTCTCAA 
CAGAAAGCAG AAGCCTCATC TCCAGTCCCT TACCTTTCGC CTGAAACAAA CCCAGCATCG 
TCAGCCTCTG CAGTTAATCC TAATGTCAGT GTAACTAATG TCAGCCTGGA GGAGCCTGCC 
CCAGCCCCTC CCGCCTCGCA CGCATCCCAG GCTGGTGGTT TAGGAGCACC AGGTGCTGAG 
GCAGCTCACG TAGTGCTCAG AGGTGAAGGA CCACCATTGC CGCCGCATGC AGACCCAGCA 
CAGGTGTATA GAAAGGAGCC GTATCCTGAA GAAATGATGA GACAAAACCA TATTTTAAAA 
CAACCAGCTC TTGGTCACCC AGGGCAGAGG CCAGATAAAG AGCCAAATCT AGCCTATGAA 
CCCCAACTTC CATATANAGA AAAACAAGCC AGCAGAGACC TTGAACAGCC GTCATACAGG 
TATGAGGCCT CAAGCTACCC AGACCAGTTT TCTCGGAACT ATGACCATCG CCTACGGTTT 
GAAGATCGAA TCCCTACCTA TGAAGACCAG TGGTCATATT ATGATGACAA ACAGCCCTAC 
CAACCTCGGC CTTTTGAGAA TCAGCATCCC CGAGACCTGG ACTCCAGACA ACATCCNGAG 
GAGGCTTCAG AACGAGGTTA TNTCCAGCGT TTTGAAGAGC CAGCCCCTCT GTCATACGAC 
AGTAGAACAC GCTATGAGCA GCTGCCTCGA ACCTCTACTC TACGACATGA AGAGCAGCCA 
GCCCCTGCAT ATGAGGTGCA CAACAGGTAC AGGCCAGAGG CACAGCCCTA TTCTTCAACA 
GGTCCTAAGT CATCTGAGCC CAAGCAGTAC TTTGACCAGT ACCCGCGAAG TTATGAGCAA 
GTACCACCAC CAGGCTTTAC CTCCAAAACA GGCCATTACG AGCCTCTCCA TGGTGCTGCA 
GTTGTCCCTC CTCTGATACC TTCCTCTCAA CATAAGCCAG AAGTCCTGCC CTCGGCTACC 
AAACCACAGC CTCCACCCCC AACCCTAACT GAGGAGGAGG AGGATCCAGC AATGAAACCA 
CAGTCCGTGC TCANCAGAGT CAAAATGTTT GAAAACAAAA GATCTGCGTC TTTGGAGAAC 
AAGAAAGATG TGAATGACAC TGCCAGCTTT AAGCCTCCAG AAGTAGCATC TAAACCTCCA 
AGTGCTTCTC TTGCTGGCCC TAAACCTGTT CCTCAGAGTC AGTTTAGTGA GCANGACAAA 
ACACTCTACA GGCTCCCAGA GCCTCAGAAA CCTCAAGTGA AGCCACCCGA NGATATTGTT 
CGATCAAATC ATTACGACCC TGAAGAGGAT GAAGAATATT ACAGGAAACA GCTCTCTTAC 
TTTGANCGAA GAAGTTTTGA GAGCAAGCCT TCTGCACATC TTCCTGCTGG CCATCACTCA 
GAGCCTGCTA AGCCAGTCCA TTCTCAGAGC CAGCCCAACT TTTCTAGTTA CTCTTCAAAG 
GGAAAACCCG AAACTGATGC TGTGGATAGA TCATTCAGTG AGAAACGTTA TGATCCAGCC 
CAGGCCACGC CTCCTCCTCC TCCGTTGCCC TCACAGTACA GCCAGCCAGC TCCCCCTCTG 
TCCAGCTCTT CTCTCCACAT ACATTCCAAG GGCGCCCAGG GCGAAGGCAA TTCAGTATCA 
TTGGATTTTC AGAACTCATA TATGTCCAAA CCAGACCCAC CCCCATCTCA GAGTAAACCA 
GCAACTTTCA GACCACCAAC CCGAGAAGAC CCTCCTCAGA CTTTCTATCC CCAGAAANGT 
TTCCCAGACA AAGCTCCAGT TAANGGAGCT GAGCAGACTC AGAAAACCAT CACTCCAGTG 
TACAATCGAT TCACACCAAA GCCGTACACA AGTTCTGCCC GGCCGTTTGA ACGCAAATTT 
GAAAGTCCGA AGTTCAACCA TAATCTTCTG CCAAGTGAAA CTGTACATAA ACCTGAATTG 
TCTTCAAAAA CTCCCACTTC TCCAAAAACT CTTATGAAAG CTCATAGTTC AACACAGCCT 
CCAGAGTTTG ACAGTGGAGT TGAAACTTTC TCTGTTCACA CAGATNAGCC TAAATATCAA 
ATGAATAATA TCAGCACCAT GCCTAAAGCT GTCCCTGTGA GTCCTTCAGC TGTGGAAGAA 
GATGAAGATG AGGATGGTCA TACTGTAGTG GCTACAGCTC GTGGCATTTT TAACAGCAAT 
GGTGGTGTGT TGAGTTCCAT AGAAACCGGT GTTAGTATAA TTATCCCACA AGGAGCCATT 
CCTGAAGGAA TTGAACAAGA AATCTATTTC AAAGTCTGCA GAGACAATAG CATTCTCCCA 
CCTTTAGATA AAGAGAAAGG TGAAACTCTG CTAAGTCCCC TAGTGATGTG TGGGCCCCAT 
GGCCTCAAGT TCCTGAAGCC CGTGGAGCTG CGCTTGCCAC ACTGTGCGTC CATGACTCCT 
GACGGTTGGT CTTTTGCTCT AAAATCATCC GACTCCTCGT CGGGTGACCC TAAAACCTGG 
CAAAACAAGT GTCTTCCTGG AGATCCGAAT TACCTTGTTG GAGCCAACTG TGTTTCTGTC 
CTGATTGACC ACTTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Tjp1 ( XM_029479118.1 ) cDNA ORF clone, Mus caroli(Ryukyu mouse) -> XP_029334978.1 Mus caroli tight junction protein 1 (Tjp1), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OMv74615
Clone ID Related Accession (Same CDS sequence)	XM_029479118.1
Accession Version	XM_029479118.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5262bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-06-09
Organism	Mus caroli(Ryukyu mouse)
Product	tight junction protein ZO-1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_034576.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus caroli Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGAAA CGGCTATATG GGAACAGCAC ACAGTGACGC TTCACAGGGC TCCTGGGTTT 
GGATTTGGAA TTGCAATCTC TGGTGGAAGA GATAATCCTC ATTTTCAGAG TGGGGAAACC 
TCCATAGTGA TTTCTGATGT GCTAAAAGGA GGGCCAGCTG AAGGACAGCT ACAGGAAAAT 
GACCGAGTTG CAATGGTTAA TGGAGTTTCA ATGGATAACG TTGAGCATGC TTTTGCTGTT 
CAGCAGCTAA GGAAAAGTGG GAAAAACGCA AAAATTACTA TCCGAAGGAA GAAGAAAGTT 
CAGATCCCTG TAAGTCACCC AGATCCTGAG CCGGTGTCTG ATAATGAAGA TGATAGCTAC 
GACGAAGAAG TACATGACCC AAGAGCTGGC CGCGGTGCTT TAGCGAACAG AAGGAGCGAG 
AAGAGCTGGG CAAGGGATAG GAGTGCAAGC AGGGAGAGGA GCCTGTCCCC TCGCTCGGAC 
AGGCGGTCGG TGGCCTCCAG TCAGCCCGCA AAACCCACCA AGGTCACACT GGTGAAGTCT 
CGGAAAAATG AAGAATATGG TCTTCGATTG GCCAGCCATA TATTTGTAAA GGAAATTTCA 
CAAGATAGTT TGGCAGCAAG AGATGGTAAC ATTCAAGAAG GGGATGTTGT CTTGAAGATA 
AATGGTACTG TGACAGAAAA TATGTCATTG ACAGATGCAA AAACACTGAT AGAAAGGTCT 
AAAGGCAAGT TAAAAATGGT AGTGCAAAGA GATGAGCGGG CTACCTTACT GAACGTCCCT 
GACCTTTCGG ATAGTATCCA TTCTGCTAAT GCCTCGGAAA GAGATGACAT TTCAGAAATT 
CAGTCACTAG CATCAGACCA TTCAGGTCGC TCGCATGACA GGCCACCCCG CCGCAGCCAG 
TCACGATCTC CTGACCAACG TTCAGAGCCC TCCGATCATT CCACACAGTC TCCACAGCAG 
CCCAGCAATG GCAGTCTCCG GAGCAGAGAG CAAGAGCGAA TGTCTAAACC TGGGGCCATC 
TCAACTCCTG TAAAACATGC AGATGATCAT ACACCCAAAG CAGTGGAAGA AGTTACAGTT 
GAGAAAAATG AGAAGCAGAC ACCCACTCTT CCAGAACCGA AGCCTGTATA TGCTCAAGTT 
GGACAACCAG ATGTGGATTT ACCCGTCAGC CCTTCTGATG GTGTTCTGCC TAATTCAACT 
CATGAAGATG GGATACTTAG GCCCAGCATG AAACTGGTAA AATTCAGAAA AGGAGATAGT 
GTGGGTTTGC GACTAGCTGG TGGAAATGAT GTCGGAATAT TTGTAGCTGG CGTTCTAGAA 
GATAGCCCTG CAGCCAAAGA AGGCTTAGAG GAAGGTGATC AAATTCTCAG GGTGAACAAT 
GTAGATTTCA CAAATATCAT AAGAGAAGAG GCTGTCCTTT TCCTCCTTGA CCTCCCTAAA 
GGTGAAGAAG TGACCATCCT GGCTCAGAAG AAGAAGGGTG TTTATCGCCG CATTGTAGAA 
TCAGATGTAG GAGATTCATT CTATATTAGA ACGCATTTTG AGTATGAAAA AGAATCTCCT 
TACGGACTTA GTTTTAACAA AGGAGAGGTG TTCCGGGTCG TGGATACTTT ATACAATGGA 
AAGCTGGGCT CTTGGCTTGC CATTCGAATT GGCAAAAATC ATAAGGAGGT AGAACGAGGC 
ATCATCCCTA ATAAGAACAG AGCTGAGCAG TTAGCCAGTG TACAGTACAC ACTTCCAAAG 
ACAGCGGGTG GTGATCGGGC AGACTTCTGG AGGTTTCGAG GTCTTCGCAG CTCCAAGAGA 
AATCTTCGAA AAAGCAGAGA GGACTTGTCA GCTCAGCCAG TTCAAACTAA GTTCCCAGCT 
TATGAAAGGG TTGTTCTTCG AGAAGCTGGA TTCCTAAGAC CTGTAACCAT CTTTGGACCA 
ATAGCTGATG TTGCCAGAGA AAAGTTGGCA AGAGAGGAAC CAGATATCTA TCAGATTGCA 
AAAAGTGAAC CACGAGATGC TGGGACTGAC CATCGTAGCT CTGGCATCAT TCGCCTTCAT 
ACAATAAAGC AAATCATAGA TCAGGATAAA CATGCGTTAT TAGATGTAAC GCCAAATGCG 
GTTGATCGTC TTAATTATGC GCAGTGGTAT CCAATTGTTG TGTTCCTTAA CCCTGACTCT 
AAGCAAGGTG TAAAAACAAT GAGGATGAGG TTATGTCCGG AGTCTCGGAA AAGCGCCAGG 
AAGCTATATG AACGCTCTCA TAAGCTTCGC AAAAACAATC ACCACCTTTT CACAACTACA 
ATTAACTTAA ACTCAATGAA TGATGGTTGG TACGGTGCCC TGAAAGAAGC CATTCAGCAG 
CAACAGAACC AGCTGGTGTG GGTCTCTGAG GGGAAGGCGG ATGGTGCTAC AAGTGATGAC 
CTTGATTTGC ATGATGATCG TCTGTCCTAC CTGTCAGCCC CAGGTAGTGA GTACTCAATG 
TATAGCACGG ACAGTAGACA CACTTCTGAC TATGAAGACA CAGATACAGA AGGCGGGGCC 
TACACTGATC AAGAACTAGA TGAAACTCTT AATGATGAGG TGGGGACTCC CCCGGAGTCT 
GCCATTACAC NGTCCTCTGA GCCTGTAAGA GAGGATTCCT CTGGGATGCA TCATGAAAAC 
CAGACCTATC CTCCTTACTC ACCACAAGCG CAGCCACAAG CTATTCATAG ACTAGACTCC 
CCTGGACTTA AGCCAGCCTC TCAACAGAAA GCAGAAGCCT CATCTCCAGT CCCTTACCTT 
TCGCCTGAAA CAAACCCAGC ATCGTCAGCC TCTGCAGTTA ATCCTAATGT CAGTGTAACT 
AATGTCAGCC TGGAGGAGCC TGCCCCAGCC CCTCCCGCCT CGCACGCATC CCAGGCTGGT 
GGTTTAGGAG CACCAGGTGC TGAGGCAGCT CACGTAGTGC TCAGAGGTGA AGGACCACCA 
TTGCCGCCGC ATGCAGACCC AGCACAGGTG TATAGAAAGG AGCCGTATCC TGAAGAAATG 
ATGAGACAAA ACCATATTTT AAAACAACCA GCTCTTGGTC ACCCAGGGCA GAGGCCAGAT 
AAAGAGCCAA ATCTAGCCTA TGAACCCCAA CTTCCATATA NAGAAAAACA AGCCAGCAGA 
GACCTTGAAC AGCCGTCATA CAGGTATGAG GCCTCAAGCT ACCCAGACCA GTTTTCTCGG 
AACTATGACC ATCGCCTACG GTTTGAAGAT CGAATCCCTA CCTATGAAGA CCAGTGGTCA 
TATTATGATG ACAAACAGCC CTACCAACCT CGGCCTTTTG AGAATCAGCA TCCCCGAGAC 
CTGGACTCCA GACAACATCC NGAGGAGGCT TCAGAACGAG GTTATNTCCA GCGTTTTGAA 
GAGCCAGCCC CTCTGTCATA CGACAGTAGA ACACGCTATG AGCAGCTGCC TCGAACCTCT 
ACTCTACGAC ATGAAGAGCA GCCAGCCCCT GCATATGAGG TGCACAACAG GTACAGGCCA 
GAGGCACAGC CCTATTCTTC AACAGGTCCT AAGTCATCTG AGCCCAAGCA GTACTTTGAC 
CAGTACCCGC GAAGTTATGA GCAAGTACCA CCACCAGGCT TTACCTCCAA AACAGGCCAT 
TACGAGCCTC TCCATGGTGC TGCAGTTGTC CCTCCTCTGA TACCTTCCTC TCAACATAAG 
CCAGAAGTCC TGCCCTCGGC TACCAAACCA CAGCCTCCAC CCCCAACCCT AACTGAGGAG 
GAGGAGGATC CAGCAATGAA ACCACAGTCC GTGCTCANCA GAGTCAAAAT GTTTGAAAAC 
AAAAGATCTG CGTCTTTGGA GAACAAGAAA GATGTGAATG ACACTGCCAG CTTTAAGCCT 
CCAGAAGTAG CATCTAAACC TCCAAGTGCT TCTCTTGCTG GCCCTAAACC TGTTCCTCAG 
AGTCAGTTTA GTGAGCANGA CAAAACACTC TACAGGCTCC CAGAGCCTCA GAAACCTCAA 
GTGAAGCCAC CCGANGATAT TGTTCGATCA AATCATTACG ACCCTGAAGA GGATGAAGAA 
TATTACAGGA AACAGCTCTC TTACTTTGAN CGAAGAAGTT TTGAGAGCAA GCCTTCTGCA 
CATCTTCCTG CTGGCCATCA CTCAGAGCCT GCTAAGCCAG TCCATTCTCA GAGCCAGCCC 
AACTTTTCTA GTTACTCTTC AAAGGGAAAA CCCGAAACTG ATGCTGTGGA TAGATCATTC 
AGTGAGAAAC GTTATGATCC AGCCCAGGCC ACGCCTCCTC CTCCTCCGTT GCCCTCACAG 
TACAGCCAGC CAGCTCCCCC TCTGTCCAGC TCTTCTCTCC ACATACATTC CAAGGGCGCC 
CAGGGCGAAG GCAATTCAGT ATCATTGGAT TTTCAGAACT CATATATGTC CAAACCAGAC 
CCACCCCCAT CTCAGAGTAA ACCAGCAACT TTCAGACCAC CAACCCGAGA AGACCCTCCT 
CAGACTTTCT ATCCCCAGAA ANGTTTCCCA GACAAAGCTC CAGTTAANGG AGCTGAGCAG 
ACTCAGAAAA CCATCACTCC AGTGTACAAT CGATTCACAC CAAAGCCGTA CACAAGTTCT 
GCCCGGCCGT TTGAACGCAA ATTTGAAAGT CCGAAGTTCA ACCATAATCT TCTGCCAAGT 
GAAACTGTAC ATAAACCTGA ATTGTCTTCA AAAACTCCCA CTTCTCCAAA AACTCTTATG 
AAAGCTCATA GTTCAACACA GCCTCCAGAG TTTGACAGTG GAGTTGAAAC TTTCTCTGTT 
CACACAGATN AGCCTAAATA TCAAATGAAT AATATCAGCA CCATGCCTAA AGCTGTCCCT 
GTGAGTCCTT CAGCTGTGGA AGAAGATGAA GATGAGGATG GTCATACTGT AGTGGCTACA 
GCTCGTGGCA TTTTTAACAG CAATGGTGGT GTGTTGAGTT CCATAGAAAC CGGTGTTAGT 
ATAATTATCC CACAAGGAGC CATTCCTGAA GGAATTGAAC AAGAAATCTA TTTCAAAGTC 
TGCAGAGACA ATAGCATTCT CCCACCTTTA GATAAAGAGA AAGGTGAAAC TCTGCTAAGT 
CCCCTAGTGA TGTGTGGGCC CCATGGCCTC AAGTTCCTGA AGCCCGTGGA GCTGCGCTTG 
CCACACTGTG CGTCCATGAC TCCTGACGGT TGGTCTTTTG CTCTAAAATC ATCCGACTCC 
TCGTCGGGTG ACCCTAAAAC CTGGCAAAAC AAGTGTCTTC CTGGAGATCC GAATTACCTT 
GTTGGAGCCA ACTGTGTTTC TGTCCTGATT GACCACTTTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_029334978.1
CDS	99..5360
Translation	MEETAIWEQHTVTLHRAPGFGFGIAISGGRDNPHFQSGETSIVISDVLKGGPAEGQLQENDRVAMVNGVSMDNVEHAFAVQQLRKSGKNAKITIRRKKKVQIPVSHPDPEPVSDNEDDSYDEEVHDPRAGRGALANRRSEKSWARDRSASRERSLSPRSDRRSVASSQPAKPTKVTLVKSRKNEEYGLRLASHIFVKEISQDSLAARDGNIQEGDVVLKINGTVTENMSLTDAKTLIERSKGKLKMVVQRDERATLLNVPDLSDSIHSANASERDDISEIQSLASDHSGRSHDRPPRRSQSRSPDQRSEPSDHSTQSPQQPSNGSLRSREQERMSKPGAISTPVKHADDHTPKAVEEVTVEKNEKQTPTLPEPKPVYAQVGQPDVDLPVSPSDGVLPNSTHEDGILRPSMKLVKFRKGDSVGLRLAGGNDVGIFVAGVLEDSPAAKEGLEEGDQILRVNNVDFTNIIREEAVLFLLDLPKGEEVTILAQKKKGVYRRIVESDVGDSFYIRTHFEYEKESPYGLSFNKGEVFRVVDTLYNGKLGSWLAIRIGKNHKEVERGIIPNKNRAEQLASVQYTLPKTAGGDRADFWRFRGLRSSKRNLRKSREDLSAQPVQTKFPAYERVVLREAGFLRPVTIFGPIADVAREKLAREEPDIYQIAKSEPRDAGTDHRSSGIIRLHTIKQIIDQDKHALLDVTPNAVDRLNYAQWYPIVVFLNPDSKQGVKTMRMRLCPESRKSARKLYERSHKLRKNNHHLFTTTINLNSMNDGWYGALKEAIQQQQNQLVWVSEGKADGATSDDLDLHDDRLSYLSAPGSEYSMYSTDSRHTSDYEDTDTEGGAYTDQELDETLNDEVGTPPESAITnullSSEPVREDSSGMHHENQTYPPYSPQAQPQAIHRLDSPGLKPASQQKAEASSPVPYLSPETNPASSASAVNPNVSVTNVSLEEPAPAPPASHASQAGGLGAPGAEAAHVVLRGEGPPLPPHADPAQVYRKEPYPEEMMRQNHILKQPALGHPGQRPDKEPNLAYEPQLPYnullEKQASRDLEQPSYRYEASSYPDQFSRNYDHRLRFEDRIPTYEDQWSYYDDKQPYQPRPFENQHPRDLDSRQHnullEEASERGYnullQRFEEPAPLSYDSRTRYEQLPRTSTLRHEEQPAPAYEVHNRYRPEAQPYSSTGPKSSEPKQYFDQYPRSYEQVPPPGFTSKTGHYEPLHGAAVVPPLIPSSQHKPEVLPSATKPQPPPPTLTEEEEDPAMKPQSVLnullRVKMFENKRSASLENKKDVNDTASFKPPEVASKPPSASLAGPKPVPQSQFSEnullDKTLYRLPEPQKPQVKPPnullDIVRSNHYDPEEDEEYYRKQLSYFnullRRSFESKPSAHLPAGHHSEPAKPVHSQSQPNFSSYSSKGKPETDAVDRSFSEKRYDPAQATPPPPPLPSQYSQPAPPLSSSSLHIHSKGAQGEGNSVSLDFQNSYMSKPDPPPSQSKPATFRPPTREDPPQTFYPQKnullFPDKAPVnullGAEQTQKTITPVYNRFTPKPYTSSARPFERKFESPKFNHNLLPSETVHKPELSSKTPTSPKTLMKAHSSTQPPEFDSGVETFSVHTDnullPKYQMNNISTMPKAVPVSPSAVEEDEDEDGHTVVATARGIFNSNGGVLSSIETGVSIIIPQGAIPEGIEQEIYFKVCRDNSILPPLDKEKGETLLSPLVMCGPHGLKFLKPVELRLPHCASMTPDGWSFALKSSDSSSGDPKTWQNKCLPGDPNYLVGANCVSVLIDHF

Target ORF information:

RefSeq Version	XM_029479118.1
Organism	Mus caroli(Ryukyu mouse)
Definition	Mus caroli tight junction protein 1 (Tjp1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_029479118.1

ORF Insert Sequence:

ATGGAGGAAA CGGCTATATG GGAACAGCAC ACAGTGACGC TTCACAGGGC TCCTGGGTTT 
GGATTTGGAA TTGCAATCTC TGGTGGAAGA GATAATCCTC ATTTTCAGAG TGGGGAAACC 
TCCATAGTGA TTTCTGATGT GCTAAAAGGA GGGCCAGCTG AAGGACAGCT ACAGGAAAAT 
GACCGAGTTG CAATGGTTAA TGGAGTTTCA ATGGATAACG TTGAGCATGC TTTTGCTGTT 
CAGCAGCTAA GGAAAAGTGG GAAAAACGCA AAAATTACTA TCCGAAGGAA GAAGAAAGTT 
CAGATCCCTG TAAGTCACCC AGATCCTGAG CCGGTGTCTG ATAATGAAGA TGATAGCTAC 
GACGAAGAAG TACATGACCC AAGAGCTGGC CGCGGTGCTT TAGCGAACAG AAGGAGCGAG 
AAGAGCTGGG CAAGGGATAG GAGTGCAAGC AGGGAGAGGA GCCTGTCCCC TCGCTCGGAC 
AGGCGGTCGG TGGCCTCCAG TCAGCCCGCA AAACCCACCA AGGTCACACT GGTGAAGTCT 
CGGAAAAATG AAGAATATGG TCTTCGATTG GCCAGCCATA TATTTGTAAA GGAAATTTCA 
CAAGATAGTT TGGCAGCAAG AGATGGTAAC ATTCAAGAAG GGGATGTTGT CTTGAAGATA 
AATGGTACTG TGACAGAAAA TATGTCATTG ACAGATGCAA AAACACTGAT AGAAAGGTCT 
AAAGGCAAGT TAAAAATGGT AGTGCAAAGA GATGAGCGGG CTACCTTACT GAACGTCCCT 
GACCTTTCGG ATAGTATCCA TTCTGCTAAT GCCTCGGAAA GAGATGACAT TTCAGAAATT 
CAGTCACTAG CATCAGACCA TTCAGGTCGC TCGCATGACA GGCCACCCCG CCGCAGCCAG 
TCACGATCTC CTGACCAACG TTCAGAGCCC TCCGATCATT CCACACAGTC TCCACAGCAG 
CCCAGCAATG GCAGTCTCCG GAGCAGAGAG CAAGAGCGAA TGTCTAAACC TGGGGCCATC 
TCAACTCCTG TAAAACATGC AGATGATCAT ACACCCAAAG CAGTGGAAGA AGTTACAGTT 
GAGAAAAATG AGAAGCAGAC ACCCACTCTT CCAGAACCGA AGCCTGTATA TGCTCAAGTT 
GGACAACCAG ATGTGGATTT ACCCGTCAGC CCTTCTGATG GTGTTCTGCC TAATTCAACT 
CATGAAGATG GGATACTTAG GCCCAGCATG AAACTGGTAA AATTCAGAAA AGGAGATAGT 
GTGGGTTTGC GACTAGCTGG TGGAAATGAT GTCGGAATAT TTGTAGCTGG CGTTCTAGAA 
GATAGCCCTG CAGCCAAAGA AGGCTTAGAG GAAGGTGATC AAATTCTCAG GGTGAACAAT 
GTAGATTTCA CAAATATCAT AAGAGAAGAG GCTGTCCTTT TCCTCCTTGA CCTCCCTAAA 
GGTGAAGAAG TGACCATCCT GGCTCAGAAG AAGAAGGGTG TTTATCGCCG CATTGTAGAA 
TCAGATGTAG GAGATTCATT CTATATTAGA ACGCATTTTG AGTATGAAAA AGAATCTCCT 
TACGGACTTA GTTTTAACAA AGGAGAGGTG TTCCGGGTCG TGGATACTTT ATACAATGGA 
AAGCTGGGCT CTTGGCTTGC CATTCGAATT GGCAAAAATC ATAAGGAGGT AGAACGAGGC 
ATCATCCCTA ATAAGAACAG AGCTGAGCAG TTAGCCAGTG TACAGTACAC ACTTCCAAAG 
ACAGCGGGTG GTGATCGGGC AGACTTCTGG AGGTTTCGAG GTCTTCGCAG CTCCAAGAGA 
AATCTTCGAA AAAGCAGAGA GGACTTGTCA GCTCAGCCAG TTCAAACTAA GTTCCCAGCT 
TATGAAAGGG TTGTTCTTCG AGAAGCTGGA TTCCTAAGAC CTGTAACCAT CTTTGGACCA 
ATAGCTGATG TTGCCAGAGA AAAGTTGGCA AGAGAGGAAC CAGATATCTA TCAGATTGCA 
AAAAGTGAAC CACGAGATGC TGGGACTGAC CATCGTAGCT CTGGCATCAT TCGCCTTCAT 
ACAATAAAGC AAATCATAGA TCAGGATAAA CATGCGTTAT TAGATGTAAC GCCAAATGCG 
GTTGATCGTC TTAATTATGC GCAGTGGTAT CCAATTGTTG TGTTCCTTAA CCCTGACTCT 
AAGCAAGGTG TAAAAACAAT GAGGATGAGG TTATGTCCGG AGTCTCGGAA AAGCGCCAGG 
AAGCTATATG AACGCTCTCA TAAGCTTCGC AAAAACAATC ACCACCTTTT CACAACTACA 
ATTAACTTAA ACTCAATGAA TGATGGTTGG TACGGTGCCC TGAAAGAAGC CATTCAGCAG 
CAACAGAACC AGCTGGTGTG GGTCTCTGAG GGGAAGGCGG ATGGTGCTAC AAGTGATGAC 
CTTGATTTGC ATGATGATCG TCTGTCCTAC CTGTCAGCCC CAGGTAGTGA GTACTCAATG 
TATAGCACGG ACAGTAGACA CACTTCTGAC TATGAAGACA CAGATACAGA AGGCGGGGCC 
TACACTGATC AAGAACTAGA TGAAACTCTT AATGATGAGG TGGGGACTCC CCCGGAGTCT 
GCCATTACAC NGTCCTCTGA GCCTGTAAGA GAGGATTCCT CTGGGATGCA TCATGAAAAC 
CAGACCTATC CTCCTTACTC ACCACAAGCG CAGCCACAAG CTATTCATAG ACTAGACTCC 
CCTGGACTTA AGCCAGCCTC TCAACAGAAA GCAGAAGCCT CATCTCCAGT CCCTTACCTT 
TCGCCTGAAA CAAACCCAGC ATCGTCAGCC TCTGCAGTTA ATCCTAATGT CAGTGTAACT 
AATGTCAGCC TGGAGGAGCC TGCCCCAGCC CCTCCCGCCT CGCACGCATC CCAGGCTGGT 
GGTTTAGGAG CACCAGGTGC TGAGGCAGCT CACGTAGTGC TCAGAGGTGA AGGACCACCA 
TTGCCGCCGC ATGCAGACCC AGCACAGGTG TATAGAAAGG AGCCGTATCC TGAAGAAATG 
ATGAGACAAA ACCATATTTT AAAACAACCA GCTCTTGGTC ACCCAGGGCA GAGGCCAGAT 
AAAGAGCCAA ATCTAGCCTA TGAACCCCAA CTTCCATATA NAGAAAAACA AGCCAGCAGA 
GACCTTGAAC AGCCGTCATA CAGGTATGAG GCCTCAAGCT ACCCAGACCA GTTTTCTCGG 
AACTATGACC ATCGCCTACG GTTTGAAGAT CGAATCCCTA CCTATGAAGA CCAGTGGTCA 
TATTATGATG ACAAACAGCC CTACCAACCT CGGCCTTTTG AGAATCAGCA TCCCCGAGAC 
CTGGACTCCA GACAACATCC NGAGGAGGCT TCAGAACGAG GTTATNTCCA GCGTTTTGAA 
GAGCCAGCCC CTCTGTCATA CGACAGTAGA ACACGCTATG AGCAGCTGCC TCGAACCTCT 
ACTCTACGAC ATGAAGAGCA GCCAGCCCCT GCATATGAGG TGCACAACAG GTACAGGCCA 
GAGGCACAGC CCTATTCTTC AACAGGTCCT AAGTCATCTG AGCCCAAGCA GTACTTTGAC 
CAGTACCCGC GAAGTTATGA GCAAGTACCA CCACCAGGCT TTACCTCCAA AACAGGCCAT 
TACGAGCCTC TCCATGGTGC TGCAGTTGTC CCTCCTCTGA TACCTTCCTC TCAACATAAG 
CCAGAAGTCC TGCCCTCGGC TACCAAACCA CAGCCTCCAC CCCCAACCCT AACTGAGGAG 
GAGGAGGATC CAGCAATGAA ACCACAGTCC GTGCTCANCA GAGTCAAAAT GTTTGAAAAC 
AAAAGATCTG CGTCTTTGGA GAACAAGAAA GATGTGAATG ACACTGCCAG CTTTAAGCCT 
CCAGAAGTAG CATCTAAACC TCCAAGTGCT TCTCTTGCTG GCCCTAAACC TGTTCCTCAG 
AGTCAGTTTA GTGAGCANGA CAAAACACTC TACAGGCTCC CAGAGCCTCA GAAACCTCAA 
GTGAAGCCAC CCGANGATAT TGTTCGATCA AATCATTACG ACCCTGAAGA GGATGAAGAA 
TATTACAGGA AACAGCTCTC TTACTTTGAN CGAAGAAGTT TTGAGAGCAA GCCTTCTGCA 
CATCTTCCTG CTGGCCATCA CTCAGAGCCT GCTAAGCCAG TCCATTCTCA GAGCCAGCCC 
AACTTTTCTA GTTACTCTTC AAAGGGAAAA CCCGAAACTG ATGCTGTGGA TAGATCATTC 
AGTGAGAAAC GTTATGATCC AGCCCAGGCC ACGCCTCCTC CTCCTCCGTT GCCCTCACAG 
TACAGCCAGC CAGCTCCCCC TCTGTCCAGC TCTTCTCTCC ACATACATTC CAAGGGCGCC 
CAGGGCGAAG GCAATTCAGT ATCATTGGAT TTTCAGAACT CATATATGTC CAAACCAGAC 
CCACCCCCAT CTCAGAGTAA ACCAGCAACT TTCAGACCAC CAACCCGAGA AGACCCTCCT 
CAGACTTTCT ATCCCCAGAA ANGTTTCCCA GACAAAGCTC CAGTTAANGG AGCTGAGCAG 
ACTCAGAAAA CCATCACTCC AGTGTACAAT CGATTCACAC CAAAGCCGTA CACAAGTTCT 
GCCCGGCCGT TTGAACGCAA ATTTGAAAGT CCGAAGTTCA ACCATAATCT TCTGCCAAGT 
GAAACTGTAC ATAAACCTGA ATTGTCTTCA AAAACTCCCA CTTCTCCAAA AACTCTTATG 
AAAGCTCATA GTTCAACACA GCCTCCAGAG TTTGACAGTG GAGTTGAAAC TTTCTCTGTT 
CACACAGATN AGCCTAAATA TCAAATGAAT AATATCAGCA CCATGCCTAA AGCTGTCCCT 
GTGAGTCCTT CAGCTGTGGA AGAAGATGAA GATGAGGATG GTCATACTGT AGTGGCTACA 
GCTCGTGGCA TTTTTAACAG CAATGGTGGT GTGTTGAGTT CCATAGAAAC CGGTGTTAGT 
ATAATTATCC CACAAGGAGC CATTCCTGAA GGAATTGAAC AAGAAATCTA TTTCAAAGTC 
TGCAGAGACA ATAGCATTCT CCCACCTTTA GATAAAGAGA AAGGTGAAAC TCTGCTAAGT 
CCCCTAGTGA TGTGTGGGCC CCATGGCCTC AAGTTCCTGA AGCCCGTGGA GCTGCGCTTG 
CCACACTGTG CGTCCATGAC TCCTGACGGT TGGTCTTTTG CTCTAAAATC ATCCGACTCC 
TCGTCGGGTG ACCCTAAAAC CTGGCAAAAC AAGTGTCTTC CTGGAGATCC GAATTACCTT 
GTTGGAGCCA ACTGTGTTTC TGTCCTGATT GACCACTTTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Tjp1 ( XM_029479119.1 ) cDNA ORF clone, Mus caroli(Ryukyu mouse) -> XP_029334979.1 Mus caroli tight junction protein 1 (Tjp1), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OMv74616
Clone ID Related Accession (Same CDS sequence)	XM_029479119.1
Accession Version	XM_029479119.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5259bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-06-09
Organism	Mus caroli(Ryukyu mouse)
Product	tight junction protein ZO-1 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_034576.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus caroli Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGTACC AGAAATACCT GACGGTGCTG CAGATGGCCA TCGGCGTCAC CCCGTCCAAC 
CGCGGCAGCC TCCTGCCGCT CAAGAGGAAG CTGTGGGTAA CTCCATCCTC TGAAAACCCG 
CATGGTGCTA CTTCCAATGT CAGCCAAGGA AAACCTTCTC TAAGACGAAT TAAAGGGAGA 
CTACATCGAA GCAAAAGCCT TGACAGTATC GACTTCTGTG AACTCACTAG CACAGCAATG 
GAGGAAACGG CTATATGGGA ACAGCACACA GTGACGCTTC ACAGGGCTCC TGGGTTTGGA 
TTTGGAATTG CAATCTCTGG TGGAAGAGAT AATCCTCATT TTCAGAGTGG GGAAACCTCC 
ATAGTGATTT CTGATGTGCT AAAAGGAGGG CCAGCTGAAG GACAGCTACA GGAAAATGAC 
CGAGTTGCAA TGGTTAATGG AGTTTCAATG GATAACGTTG AGCATGCTTT TGCTGTTCAG 
CAGCTAAGGA AAAGTGGGAA AAACGCAAAA ATTACTATCC GAAGGAAGAA GAAAGTTCAG 
ATCCCTGTAA GTCACCCAGA TCCTGAGCCG GTGTCTGATA ATGAAGATGA TAGCTACGAC 
GAAGAAGTAC ATGACCCAAG AGCTGGCCGC GGTGCTTTAG CGAACAGAAG GAGCGAGAAG 
AGCTGGGCAA GGGATAGGAG TGCAAGCAGG GAGAGGAGCC TGTCCCCTCG CTCGGACAGG 
CGGTCGGTGG CCTCCAGTCA GCCCGCAAAA CCCACCAAGG TCACACTGGT GAAGTCTCGG 
AAAAATGAAG AATATGGTCT TCGATTGGCC AGCCATATAT TTGTAAAGGA AATTTCACAA 
GATAGTTTGG CAGCAAGAGA TGGTAACATT CAAGAAGGGG ATGTTGTCTT GAAGATAAAT 
GGTACTGTGA CAGAAAATAT GTCATTGACA GATGCAAAAA CACTGATAGA AAGGTCTAAA 
GGCAAGTTAA AAATGGTAGT GCAAAGAGAT GAGCGGGCTA CCTTACTGAA CGTCCCTGAC 
CTTTCGGATA GTATCCATTC TGCTAATGCC TCGGAAAGAG ATGACATTTC AGAAATTCAG 
TCACTAGCAT CAGACCATTC AGGTCGCTCG CATGACAGGC CACCCCGCCG CAGCCAGTCA 
CGATCTCCTG ACCAACGTTC AGAGCCCTCC GATCATTCCA CACAGTCTCC ACAGCAGCCC 
AGCAATGGCA GTCTCCGGAG CAGAGAGCAA GAGCGAATGT CTAAACCTGG GGCCATCTCA 
ACTCCTGTAA AACATGCAGA TGATCATACA CCCAAAGCAG TGGAAGAAGT TACAGTTGAG 
AAAAATGAGA AGCAGACACC CACTCTTCCA GAACCGAAGC CTGTATATGC TCAAGTTGGA 
CAACCAGATG TGGATTTACC CGTCAGCCCT TCTGATGGTG TTCTGCCTAA TTCAACTCAT 
GAAGATGGGA TACTTAGGCC CAGCATGAAA CTGGTAAAAT TCAGAAAAGG AGATAGTGTG 
GGTTTGCGAC TAGCTGGTGG AAATGATGTC GGAATATTTG TAGCTGGCGT TCTAGAAGAT 
AGCCCTGCAG CCAAAGAAGG CTTAGAGGAA GGTGATCAAA TTCTCAGGGT GAACAATGTA 
GATTTCACAA ATATCATAAG AGAAGAGGCT GTCCTTTTCC TCCTTGACCT CCCTAAAGGT 
GAAGAAGTGA CCATCCTGGC TCAGAAGAAG AAGGGTGTTT ATCGCCGCAT TGTAGAATCA 
GATGTAGGAG ATTCATTCTA TATTAGAACG CATTTTGAGT ATGAAAAAGA ATCTCCTTAC 
GGACTTAGTT TTAACAAAGG AGAGGTGTTC CGGGTCGTGG ATACTTTATA CAATGGAAAG 
CTGGGCTCTT GGCTTGCCAT TCGAATTGGC AAAAATCATA AGGAGGTAGA ACGAGGCATC 
ATCCCTAATA AGAACAGAGC TGAGCAGTTA GCCAGTGTAC AGTACACACT TCCAAAGACA 
GCGGGTGGTG ATCGGGCAGA CTTCTGGAGG TTTCGAGGTC TTCGCAGCTC CAAGAGAAAT 
CTTCGAAAAA GCAGAGAGGA CTTGTCAGCT CAGCCAGTTC AAACTAAGTT CCCAGCTTAT 
GAAAGGGTTG TTCTTCGAGA AGCTGGATTC CTAAGACCTG TAACCATCTT TGGACCAATA 
GCTGATGTTG CCAGAGAAAA GTTGGCAAGA GAGGAACCAG ATATCTATCA GATTGCAAAA 
AGTGAACCAC GAGATGCTGG GACTGACCAT CGTAGCTCTG GCATCATTCG CCTTCATACA 
ATAAAGCAAA TCATAGATCA GGATAAACAT GCGTTATTAG ATGTAACGCC AAATGCGGTT 
GATCGTCTTA ATTATGCGCA GTGGTATCCA ATTGTTGTGT TCCTTAACCC TGACTCTAAG 
CAAGGTGTAA AAACAATGAG GATGAGGTTA TGTCCGGAGT CTCGGAAAAG CGCCAGGAAG 
CTATATGAAC GCTCTCATAA GCTTCGCAAA AACAATCACC ACCTTTTCAC AACTACAATT 
AACTTAAACT CAATGAATGA TGGTTGGTAC GGTGCCCTGA AAGAAGCCAT TCAGCAGCAA 
CAGAACCAGC TGGTGTGGGT CTCTGAGGGG AAGGCGGATG GTGCTACAAG TGATGACCTT 
GATTTGCATG ATGATCGTCT GTCCTACCTG TCAGCCCCAG GTAGTGAGTA CTCAATGTAT 
AGCACGGACA GTAGACACAC TTCTGACTAT GAAGACACAG ATACAGAAGG CGGGGCCTAC 
ACTGATCAAG AACTAGATGA AACTCTTAAT GATGAGGTGG GGACTCCCCC GGAGTCTGCC 
ATTACACNGT CCTCTGAGCC TGTAAGAGAG GATTCCTCTG GGATGCATCA TGAAAACCAG 
ACCTATCCTC CTTACTCACC ACAAGCGCAG CCACAAGCTA TTCATAGACT AGACTCCCCT 
GGACTTAAGC CAGCCTCTCA ACAGGTGTAT AGAAAGGAGC CGTATCCTGA AGAAATGATG 
AGACAAAACC ATATTTTAAA ACAACCAGCT CTTGGTCACC CAGGGCAGAG GCCAGATAAA 
GAGCCAAATC TAGCCTATGA ACCCCAACTT CCATATANAG AAAAACAAGC CAGCAGAGAC 
CTTGAACAGC CGTCATACAG GTATGAGGCC TCAAGCTACC CAGACCAGTT TTCTCGGAAC 
TATGACCATC GCCTACGGTT TGAAGATCGA ATCCCTACCT ATGAAGACCA GTGGTCATAT 
TATGATGACA AACAGCCCTA CCAACCTCGG CCTTTTGAGA ATCAGCATCC CCGAGACCTG 
GACTCCAGAC AACATCCNGA GGAGGCTTCA GAACGAGGTT ATNTCCAGCG TTTTGAAGAG 
CCAGCCCCTC TGTCATACGA CAGTAGAACA CGCTATGAGC AGCTGCCTCG AACCTCTACT 
CTACGACATG AAGAGCAGCC AGCCCCTGCA TATGAGGTGC ACAACAGGTA CAGGCCAGAG 
GCACAGCCCT ATTCTTCAAC AGGTCCTAAG TCATCTGAGC CCAAGCAGTA CTTTGACCAG 
TACCCGCGAA GTTATGAGCA AGTACCACCA CCAGGCTTTA CCTCCAAAAC AGGCCATTAC 
GAGCCTCTCC ATGGTGCTGC AGTTGTCCCT CCTCTGATAC CTTCCTCTCA ACATAAGCCA 
GAAGTCCTGC CCTCGGCTAC CAAACCACAG CCTCCACCCC CAACCCTAAC TGAGGAGGAG 
GAGGATCCAG CAATGAAACC ACAGTCCGTG CTCANCAGAG TCAAAATGTT TGAAAACAAA 
AGATCTGCGT CTTTGGAGAA CAAGAAAGAT GTGAATGACA CTGCCAGCTT TAAGCCTCCA 
GAAGTAGCAT CTAAACCTCC AAGTGCTTCT CTTGCTGGCC CTAAACCTGT TCCTCAGAGT 
CAGTTTAGTG AGCANGACAA AACACTCTAC AGGCTCCCAG AGCCTCAGAA ACCTCAAGTG 
AAGCCACCCG ANGATATTGT TCGATCAAAT CATTACGACC CTGAAGAGGA TGAAGAATAT 
TACAGGAAAC AGCTCTCTTA CTTTGANCGA AGAAGTTTTG AGAGCAAGCC TTCTGCACAT 
CTTCCTGCTG GCCATCACTC AGAGCCTGCT AAGCCAGTCC ATTCTCAGAG CCAGCCCAAC 
TTTTCTAGTT ACTCTTCAAA GGGAAAACCC GAAACTGATG CTGTGGATAG ATCATTCAGT 
GAGAAACGTT ATGATCCAGC CCAGGCCACG CCTCCTCCTC CTCCGTTGCC CTCACAGTAC 
AGCCAGCCAG CTCCCCCTCT GTCCAGCTCT TCTCTCCACA TACATTCCAA GGGCGCCCAG 
GGCGAAGGCA ATTCAGTATC ATTGGATTTT CAGAACTCAT ATATGTCCAA ACCAGACCCA 
CCCCCATCTC AGAGTAAACC AGCAACTTTC AGACCACCAA CCCGAGAAGA CCCTCCTCAG 
ACTTTCTATC CCCAGAAANG TTTCCCAGAC AAAGCTCCAG TTAANGGAGC TGAGCAGACT 
CAGAAAACCA TCACTCCAGT GTACAATCGA TTCACACCAA AGCCGTACAC AAGTTCTGCC 
CGGCCGTTTG AACGCAAATT TGAAAGTCCG AAGTTCAACC ATAATCTTCT GCCAAGTGAA 
ACTGTACATA AACCTGAATT GTCTTCAAAA ACTCCCACTT CTCCAAAAAC TCTTATGAAA 
GCTCATAGTT CAACACAGCC TCCAGAGTTT GACAGTGGAG TTGAAACTTT CTCTGTTCAC 
ACAGATNAGC CTAAATATCA AATGAATAAT ATCAGCACCA TGCCTAAAGC TGTCCCTGTG 
AGTCCTTCAG CTGTGGAAGA AGATGAAGAT GAGGATGGTC ATACTGTAGT GGCTACAGCT 
CGTGGCATTT TTAACAGCAA TGGTGGTGTG TTGAGTTCCA TAGAAACCGG TGTTAGTATA 
ATTATCCCAC AAGGAGCCAT TCCTGAAGGA ATTGAACAAG AAATCTATTT CAAAGTCTGC 
AGAGACAATA GCATTCTCCC ACCTTTAGAT AAAGAGAAAG GTGAAACTCT GCTAAGTCCC 
CTAGTGATGT GTGGGCCCCA TGGCCTCAAG TTCCTGAAGC CCGTGGAGCT GCGCTTGCCA 
CACTGTGCGT CCATGACTCC TGACGGTTGG TCTTTTGCTC TAAAATCATC CGACTCCTCG 
TCGGGTGACC CTAAAACCTG GCAAAACAAG TGTCTTCCTG GAGATCCGAA TTACCTTGTT 
GGAGCCAACT GTGTTTCTGT CCTGATTGAC CACTTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_029334979.1
CDS	68..5326
Translation	MKYQKYLTVLQMAIGVTPSNRGSLLPLKRKLWVTPSSENPHGATSNVSQGKPSLRRIKGRLHRSKSLDSIDFCELTSTAMEETAIWEQHTVTLHRAPGFGFGIAISGGRDNPHFQSGETSIVISDVLKGGPAEGQLQENDRVAMVNGVSMDNVEHAFAVQQLRKSGKNAKITIRRKKKVQIPVSHPDPEPVSDNEDDSYDEEVHDPRAGRGALANRRSEKSWARDRSASRERSLSPRSDRRSVASSQPAKPTKVTLVKSRKNEEYGLRLASHIFVKEISQDSLAARDGNIQEGDVVLKINGTVTENMSLTDAKTLIERSKGKLKMVVQRDERATLLNVPDLSDSIHSANASERDDISEIQSLASDHSGRSHDRPPRRSQSRSPDQRSEPSDHSTQSPQQPSNGSLRSREQERMSKPGAISTPVKHADDHTPKAVEEVTVEKNEKQTPTLPEPKPVYAQVGQPDVDLPVSPSDGVLPNSTHEDGILRPSMKLVKFRKGDSVGLRLAGGNDVGIFVAGVLEDSPAAKEGLEEGDQILRVNNVDFTNIIREEAVLFLLDLPKGEEVTILAQKKKGVYRRIVESDVGDSFYIRTHFEYEKESPYGLSFNKGEVFRVVDTLYNGKLGSWLAIRIGKNHKEVERGIIPNKNRAEQLASVQYTLPKTAGGDRADFWRFRGLRSSKRNLRKSREDLSAQPVQTKFPAYERVVLREAGFLRPVTIFGPIADVAREKLAREEPDIYQIAKSEPRDAGTDHRSSGIIRLHTIKQIIDQDKHALLDVTPNAVDRLNYAQWYPIVVFLNPDSKQGVKTMRMRLCPESRKSARKLYERSHKLRKNNHHLFTTTINLNSMNDGWYGALKEAIQQQQNQLVWVSEGKADGATSDDLDLHDDRLSYLSAPGSEYSMYSTDSRHTSDYEDTDTEGGAYTDQELDETLNDEVGTPPESAITnullSSEPVREDSSGMHHENQTYPPYSPQAQPQAIHRLDSPGLKPASQQVYRKEPYPEEMMRQNHILKQPALGHPGQRPDKEPNLAYEPQLPYnullEKQASRDLEQPSYRYEASSYPDQFSRNYDHRLRFEDRIPTYEDQWSYYDDKQPYQPRPFENQHPRDLDSRQHnullEEASERGYnullQRFEEPAPLSYDSRTRYEQLPRTSTLRHEEQPAPAYEVHNRYRPEAQPYSSTGPKSSEPKQYFDQYPRSYEQVPPPGFTSKTGHYEPLHGAAVVPPLIPSSQHKPEVLPSATKPQPPPPTLTEEEEDPAMKPQSVLnullRVKMFENKRSASLENKKDVNDTASFKPPEVASKPPSASLAGPKPVPQSQFSEnullDKTLYRLPEPQKPQVKPPnullDIVRSNHYDPEEDEEYYRKQLSYFnullRRSFESKPSAHLPAGHHSEPAKPVHSQSQPNFSSYSSKGKPETDAVDRSFSEKRYDPAQATPPPPPLPSQYSQPAPPLSSSSLHIHSKGAQGEGNSVSLDFQNSYMSKPDPPPSQSKPATFRPPTREDPPQTFYPQKnullFPDKAPVnullGAEQTQKTITPVYNRFTPKPYTSSARPFERKFESPKFNHNLLPSETVHKPELSSKTPTSPKTLMKAHSSTQPPEFDSGVETFSVHTDnullPKYQMNNISTMPKAVPVSPSAVEEDEDEDGHTVVATARGIFNSNGGVLSSIETGVSIIIPQGAIPEGIEQEIYFKVCRDNSILPPLDKEKGETLLSPLVMCGPHGLKFLKPVELRLPHCASMTPDGWSFALKSSDSSSGDPKTWQNKCLPGDPNYLVGANCVSVLIDHF

Target ORF information:

RefSeq Version	XM_029479119.1
Organism	Mus caroli(Ryukyu mouse)
Definition	Mus caroli tight junction protein 1 (Tjp1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_029479119.1

ORF Insert Sequence:

ATGAAGTACC AGAAATACCT GACGGTGCTG CAGATGGCCA TCGGCGTCAC CCCGTCCAAC 
CGCGGCAGCC TCCTGCCGCT CAAGAGGAAG CTGTGGGTAA CTCCATCCTC TGAAAACCCG 
CATGGTGCTA CTTCCAATGT CAGCCAAGGA AAACCTTCTC TAAGACGAAT TAAAGGGAGA 
CTACATCGAA GCAAAAGCCT TGACAGTATC GACTTCTGTG AACTCACTAG CACAGCAATG 
GAGGAAACGG CTATATGGGA ACAGCACACA GTGACGCTTC ACAGGGCTCC TGGGTTTGGA 
TTTGGAATTG CAATCTCTGG TGGAAGAGAT AATCCTCATT TTCAGAGTGG GGAAACCTCC 
ATAGTGATTT CTGATGTGCT AAAAGGAGGG CCAGCTGAAG GACAGCTACA GGAAAATGAC 
CGAGTTGCAA TGGTTAATGG AGTTTCAATG GATAACGTTG AGCATGCTTT TGCTGTTCAG 
CAGCTAAGGA AAAGTGGGAA AAACGCAAAA ATTACTATCC GAAGGAAGAA GAAAGTTCAG 
ATCCCTGTAA GTCACCCAGA TCCTGAGCCG GTGTCTGATA ATGAAGATGA TAGCTACGAC 
GAAGAAGTAC ATGACCCAAG AGCTGGCCGC GGTGCTTTAG CGAACAGAAG GAGCGAGAAG 
AGCTGGGCAA GGGATAGGAG TGCAAGCAGG GAGAGGAGCC TGTCCCCTCG CTCGGACAGG 
CGGTCGGTGG CCTCCAGTCA GCCCGCAAAA CCCACCAAGG TCACACTGGT GAAGTCTCGG 
AAAAATGAAG AATATGGTCT TCGATTGGCC AGCCATATAT TTGTAAAGGA AATTTCACAA 
GATAGTTTGG CAGCAAGAGA TGGTAACATT CAAGAAGGGG ATGTTGTCTT GAAGATAAAT 
GGTACTGTGA CAGAAAATAT GTCATTGACA GATGCAAAAA CACTGATAGA AAGGTCTAAA 
GGCAAGTTAA AAATGGTAGT GCAAAGAGAT GAGCGGGCTA CCTTACTGAA CGTCCCTGAC 
CTTTCGGATA GTATCCATTC TGCTAATGCC TCGGAAAGAG ATGACATTTC AGAAATTCAG 
TCACTAGCAT CAGACCATTC AGGTCGCTCG CATGACAGGC CACCCCGCCG CAGCCAGTCA 
CGATCTCCTG ACCAACGTTC AGAGCCCTCC GATCATTCCA CACAGTCTCC ACAGCAGCCC 
AGCAATGGCA GTCTCCGGAG CAGAGAGCAA GAGCGAATGT CTAAACCTGG GGCCATCTCA 
ACTCCTGTAA AACATGCAGA TGATCATACA CCCAAAGCAG TGGAAGAAGT TACAGTTGAG 
AAAAATGAGA AGCAGACACC CACTCTTCCA GAACCGAAGC CTGTATATGC TCAAGTTGGA 
CAACCAGATG TGGATTTACC CGTCAGCCCT TCTGATGGTG TTCTGCCTAA TTCAACTCAT 
GAAGATGGGA TACTTAGGCC CAGCATGAAA CTGGTAAAAT TCAGAAAAGG AGATAGTGTG 
GGTTTGCGAC TAGCTGGTGG AAATGATGTC GGAATATTTG TAGCTGGCGT TCTAGAAGAT 
AGCCCTGCAG CCAAAGAAGG CTTAGAGGAA GGTGATCAAA TTCTCAGGGT GAACAATGTA 
GATTTCACAA ATATCATAAG AGAAGAGGCT GTCCTTTTCC TCCTTGACCT CCCTAAAGGT 
GAAGAAGTGA CCATCCTGGC TCAGAAGAAG AAGGGTGTTT ATCGCCGCAT TGTAGAATCA 
GATGTAGGAG ATTCATTCTA TATTAGAACG CATTTTGAGT ATGAAAAAGA ATCTCCTTAC 
GGACTTAGTT TTAACAAAGG AGAGGTGTTC CGGGTCGTGG ATACTTTATA CAATGGAAAG 
CTGGGCTCTT GGCTTGCCAT TCGAATTGGC AAAAATCATA AGGAGGTAGA ACGAGGCATC 
ATCCCTAATA AGAACAGAGC TGAGCAGTTA GCCAGTGTAC AGTACACACT TCCAAAGACA 
GCGGGTGGTG ATCGGGCAGA CTTCTGGAGG TTTCGAGGTC TTCGCAGCTC CAAGAGAAAT 
CTTCGAAAAA GCAGAGAGGA CTTGTCAGCT CAGCCAGTTC AAACTAAGTT CCCAGCTTAT 
GAAAGGGTTG TTCTTCGAGA AGCTGGATTC CTAAGACCTG TAACCATCTT TGGACCAATA 
GCTGATGTTG CCAGAGAAAA GTTGGCAAGA GAGGAACCAG ATATCTATCA GATTGCAAAA 
AGTGAACCAC GAGATGCTGG GACTGACCAT CGTAGCTCTG GCATCATTCG CCTTCATACA 
ATAAAGCAAA TCATAGATCA GGATAAACAT GCGTTATTAG ATGTAACGCC AAATGCGGTT 
GATCGTCTTA ATTATGCGCA GTGGTATCCA ATTGTTGTGT TCCTTAACCC TGACTCTAAG 
CAAGGTGTAA AAACAATGAG GATGAGGTTA TGTCCGGAGT CTCGGAAAAG CGCCAGGAAG 
CTATATGAAC GCTCTCATAA GCTTCGCAAA AACAATCACC ACCTTTTCAC AACTACAATT 
AACTTAAACT CAATGAATGA TGGTTGGTAC GGTGCCCTGA AAGAAGCCAT TCAGCAGCAA 
CAGAACCAGC TGGTGTGGGT CTCTGAGGGG AAGGCGGATG GTGCTACAAG TGATGACCTT 
GATTTGCATG ATGATCGTCT GTCCTACCTG TCAGCCCCAG GTAGTGAGTA CTCAATGTAT 
AGCACGGACA GTAGACACAC TTCTGACTAT GAAGACACAG ATACAGAAGG CGGGGCCTAC 
ACTGATCAAG AACTAGATGA AACTCTTAAT GATGAGGTGG GGACTCCCCC GGAGTCTGCC 
ATTACACNGT CCTCTGAGCC TGTAAGAGAG GATTCCTCTG GGATGCATCA TGAAAACCAG 
ACCTATCCTC CTTACTCACC ACAAGCGCAG CCACAAGCTA TTCATAGACT AGACTCCCCT 
GGACTTAAGC CAGCCTCTCA ACAGGTGTAT AGAAAGGAGC CGTATCCTGA AGAAATGATG 
AGACAAAACC ATATTTTAAA ACAACCAGCT CTTGGTCACC CAGGGCAGAG GCCAGATAAA 
GAGCCAAATC TAGCCTATGA ACCCCAACTT CCATATANAG AAAAACAAGC CAGCAGAGAC 
CTTGAACAGC CGTCATACAG GTATGAGGCC TCAAGCTACC CAGACCAGTT TTCTCGGAAC 
TATGACCATC GCCTACGGTT TGAAGATCGA ATCCCTACCT ATGAAGACCA GTGGTCATAT 
TATGATGACA AACAGCCCTA CCAACCTCGG CCTTTTGAGA ATCAGCATCC CCGAGACCTG 
GACTCCAGAC AACATCCNGA GGAGGCTTCA GAACGAGGTT ATNTCCAGCG TTTTGAAGAG 
CCAGCCCCTC TGTCATACGA CAGTAGAACA CGCTATGAGC AGCTGCCTCG AACCTCTACT 
CTACGACATG AAGAGCAGCC AGCCCCTGCA TATGAGGTGC ACAACAGGTA CAGGCCAGAG 
GCACAGCCCT ATTCTTCAAC AGGTCCTAAG TCATCTGAGC CCAAGCAGTA CTTTGACCAG 
TACCCGCGAA GTTATGAGCA AGTACCACCA CCAGGCTTTA CCTCCAAAAC AGGCCATTAC 
GAGCCTCTCC ATGGTGCTGC AGTTGTCCCT CCTCTGATAC CTTCCTCTCA ACATAAGCCA 
GAAGTCCTGC CCTCGGCTAC CAAACCACAG CCTCCACCCC CAACCCTAAC TGAGGAGGAG 
GAGGATCCAG CAATGAAACC ACAGTCCGTG CTCANCAGAG TCAAAATGTT TGAAAACAAA 
AGATCTGCGT CTTTGGAGAA CAAGAAAGAT GTGAATGACA CTGCCAGCTT TAAGCCTCCA 
GAAGTAGCAT CTAAACCTCC AAGTGCTTCT CTTGCTGGCC CTAAACCTGT TCCTCAGAGT 
CAGTTTAGTG AGCANGACAA AACACTCTAC AGGCTCCCAG AGCCTCAGAA ACCTCAAGTG 
AAGCCACCCG ANGATATTGT TCGATCAAAT CATTACGACC CTGAAGAGGA TGAAGAATAT 
TACAGGAAAC AGCTCTCTTA CTTTGANCGA AGAAGTTTTG AGAGCAAGCC TTCTGCACAT 
CTTCCTGCTG GCCATCACTC AGAGCCTGCT AAGCCAGTCC ATTCTCAGAG CCAGCCCAAC 
TTTTCTAGTT ACTCTTCAAA GGGAAAACCC GAAACTGATG CTGTGGATAG ATCATTCAGT 
GAGAAACGTT ATGATCCAGC CCAGGCCACG CCTCCTCCTC CTCCGTTGCC CTCACAGTAC 
AGCCAGCCAG CTCCCCCTCT GTCCAGCTCT TCTCTCCACA TACATTCCAA GGGCGCCCAG 
GGCGAAGGCA ATTCAGTATC ATTGGATTTT CAGAACTCAT ATATGTCCAA ACCAGACCCA 
CCCCCATCTC AGAGTAAACC AGCAACTTTC AGACCACCAA CCCGAGAAGA CCCTCCTCAG 
ACTTTCTATC CCCAGAAANG TTTCCCAGAC AAAGCTCCAG TTAANGGAGC TGAGCAGACT 
CAGAAAACCA TCACTCCAGT GTACAATCGA TTCACACCAA AGCCGTACAC AAGTTCTGCC 
CGGCCGTTTG AACGCAAATT TGAAAGTCCG AAGTTCAACC ATAATCTTCT GCCAAGTGAA 
ACTGTACATA AACCTGAATT GTCTTCAAAA ACTCCCACTT CTCCAAAAAC TCTTATGAAA 
GCTCATAGTT CAACACAGCC TCCAGAGTTT GACAGTGGAG TTGAAACTTT CTCTGTTCAC 
ACAGATNAGC CTAAATATCA AATGAATAAT ATCAGCACCA TGCCTAAAGC TGTCCCTGTG 
AGTCCTTCAG CTGTGGAAGA AGATGAAGAT GAGGATGGTC ATACTGTAGT GGCTACAGCT 
CGTGGCATTT TTAACAGCAA TGGTGGTGTG TTGAGTTCCA TAGAAACCGG TGTTAGTATA 
ATTATCCCAC AAGGAGCCAT TCCTGAAGGA ATTGAACAAG AAATCTATTT CAAAGTCTGC 
AGAGACAATA GCATTCTCCC ACCTTTAGAT AAAGAGAAAG GTGAAACTCT GCTAAGTCCC 
CTAGTGATGT GTGGGCCCCA TGGCCTCAAG TTCCTGAAGC CCGTGGAGCT GCGCTTGCCA 
CACTGTGCGT CCATGACTCC TGACGGTTGG TCTTTTGCTC TAAAATCATC CGACTCCTCG 
TCGGGTGACC CTAAAACCTG GCAAAACAAG TGTCTTCCTG GAGATCCGAA TTACCTTGTT 
GGAGCCAACT GTGTTTCTGT CCTGATTGAC CACTTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Tjp1 ( XM_029479120.1 ) cDNA ORF clone, Mus caroli(Ryukyu mouse) -> XP_029334980.1 Mus caroli tight junction protein 1 (Tjp1), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OMv74617
Clone ID Related Accession (Same CDS sequence)	XM_029479120.1
Accession Version	XM_029479120.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5199bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-06-09
Organism	Mus caroli(Ryukyu mouse)
Product	tight junction protein ZO-1 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_034576.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus caroli Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGTACC AGAAATACCT GACGGTGCTG CAGATGGCCA TCGGCGTCAC CCCGTCCAAC 
CGCGGCAGCC TCCTGCCGCT CAAGAGGAAG CTGTGGGTAA CTCCATCCTC TGAAAACCCG 
CATGGTGCTA CTTCCAATGT CAGCCAAGGA AAACCTTCTC TAAGACGAAT TAAAGGGAGA 
CTACATCGAA GCAAAAGCCT TGACAGTATC GACTTCTGTG AACTCACTAG CACAGCAATG 
GAGGAAACGG CTATATGGGA ACAGCACACA GTGACGCTTC ACAGGGCTCC TGGGTTTGGA 
TTTGGAATTG CAATCTCTGG TGGAAGAGAT AATCCTCATT TTCAGAGTGG GGAAACCTCC 
ATAGTGATTT CTGATGTGCT AAAAGGAGGG CCAGCTGAAG GACAGCTACA GGAAAATGAC 
CGAGTTGCAA TGGTTAATGG AGTTTCAATG GATAACGTTG AGCATGCTTT TGCTGTTCAG 
CAGCTAAGGA AAAGTGGGAA AAACGCAAAA ATTACTATCC GAAGGAAGAA GAAAGTTCAG 
ATCCCTGTAA GTCACCCAGA TCCTGAGCCG GTGTCTGATA ATGAAGATGA TAGCTACGAC 
GAAGAAGTAC ATGACCCAAG AGCTGGCCGC GGTGCTTTAG CGAACAGAAG GAGCGAGAAG 
AGCTGGGCAA GGGATAGGAG TGCAAGCAGG GAGAGGAGCC TGTCCCCTCG CTCGGACAGG 
CGGTCGGTGG CCTCCAGTCA GCCCGCAAAA CCCACCAAGG TCACACTGGT GAAGTCTCGG 
AAAAATGAAG AATATGGTCT TCGATTGGCC AGCCATATAT TTGTAAAGGA AATTTCACAA 
GATAGTTTGG CAGCAAGAGA TGGTAACATT CAAGAAGGGG ATGTTGTCTT GAAGATAAAT 
GGTACTGTGA CAGAAAATAT GTCATTGACA GATGCAAAAA CACTGATAGA AAGGTCTAAA 
GGCAAGTTAA AAATGGTAGT GCAAAGAGAT GAGCGGGCTA CCTTACTGAA CGTCCCTGAC 
CTTTCGGATA GTATCCATTC TGCTAATGCC TCGGAAAGAG ATGACATTTC AGAAATTCAG 
TCACTAGCAT CAGACCATTC AGGTCGCTCG CATGACAGGC CACCCCGCCG CAGCCAGTCA 
CGATCTCCTG ACCAACGTTC AGAGCCCTCC GATCATTCCA CACAGTCTCC ACAGCAGCCC 
AGCAATGGCA GTCTCCGGAG CAGAGAGCAA GAGCGAATGT CTAAACCTGG GGCCATCTCA 
ACTCCTGTAA AACATGCAGA TGATCATACA CCCAAAGCAG TGGAAGAAGT TACAGTTGAG 
AAAAATGAGA AGCAGACACC CACTCTTCCA GAACCGAAGC CTGTATATGC TCAAGTTGGA 
CAACCAGATG TGGATTTACC CGTCAGCCCT TCTGATGGTG TTCTGCCTAA TTCAACTCAT 
GAAGATGGGA TACTTAGGCC CAGCATGAAA CTGGTAAAAT TCAGAAAAGG AGATAGTGTG 
GGTTTGCGAC TAGCTGGTGG AAATGATGTC GGAATATTTG TAGCTGGCGT TCTAGAAGAT 
AGCCCTGCAG CCAAAGAAGG CTTAGAGGAA GGTGATCAAA TTCTCAGGGT GAACAATGTA 
GATTTCACAA ATATCATAAG AGAAGAGGCT GTCCTTTTCC TCCTTGACCT CCCTAAAGGT 
GAAGAAGTGA CCATCCTGGC TCAGAAGAAG AAGGGTGTTT ATCGCCGCAT TGTAGAATCA 
GATGTAGGAG ATTCATTCTA TATTAGAACG CATTTTGAGT ATGAAAAAGA ATCTCCTTAC 
GGACTTAGTT TTAACAAAGG AGAGGTGTTC CGGGTCGTGG ATACTTTATA CAATGGAAAG 
CTGGGCTCTT GGCTTGCCAT TCGAATTGGC AAAAATCATA AGGAGGTAGA ACGAGGCATC 
ATCCCTAATA AGAACAGAGC TGAGCAGTTA GCCAGTGTAC AGTACACACT TCCAAAGACA 
GCGGGTGGTG ATCGGGCAGA CTTCTGGAGG TTTCGAGGTC TTCGCAGCTC CAAGAGAAAT 
CTTCGAAAAA GCAGAGAGGA CTTGTCAGCT CAGCCAGTTC AAACTAAGTT CCCAGCTTAT 
GAAAGGGTTG TTCTTCGAGA AGCTGGATTC CTAAGACCTG TAACCATCTT TGGACCAATA 
GCTGATGTTG CCAGAGAAAA GTTGGCAAGA GAGGAACCAG ATATCTATCA GATTGCAAAA 
AGTGAACCAC GAGATGCTGG GACTGACCAT CGTAGCTCTG GCATCATTCG CCTTCATACA 
ATAAAGCAAA TCATAGATCA GGATAAACAT GCGTTATTAG ATGTAACGCC AAATGCGGTT 
GATCGTCTTA ATTATGCGCA GTGGTATCCA ATTGTTGTGT TCCTTAACCC TGACTCTAAG 
CAAGGTGTAA AAACAATGAG GATGAGGTTA TGTCCGGAGT CTCGGAAAAG CGCCAGGAAG 
CTATATGAAC GCTCTCATAA GCTTCGCAAA AACAATCACC ACCTTTTCAC AACTACAATT 
AACTTAAACT CAATGAATGA TGGTTGGTAC GGTGCCCTGA AAGAAGCCAT TCAGCAGCAA 
CAGAACCAGC TGGTGTGGGT CTCTGAGGGG AAGGCGGATG GTGCTACAAG TGATGACCTT 
GATTTGCATG ATGATCGTCT GTCCTACCTG TCAGCCCCAG GTAGTGAGTA CTCAATGTAT 
AGCACGGACA GTAGACACAC TTCTGACTAT GAAGACACAG ATACAGAAGG CGGGGCCTAC 
ACTGATCAAG AACTAGATGA AACTCTTAAT GATGAGGTGG GGACTCCCCC GGAGTCTGCC 
ATTACACNGT CCTCTGAGCC TGTAAGAGAG GATTCCTCTG GGATGCATCA TGAAAACCAG 
ACCTATCCTC CTTACTCACC ACAAGCGCAG CCACAAGCTA TTCATAGACT AGACTCCCCT 
GGACTTAAGC CAGCCTCTCA ACAGGTGTAT AGAAAGGAGC CGTATCCTGA AGAAATGATG 
AGACAAAACC ATATTTTAAA ACAACCAGCT CTTGGTCACC CAGGGCAGAG GCCAGATAAA 
GAGCCAAATC TAGCCTATGA ACCCCAACTT CCATATANAG AAAAACAAGC CAGCAGAGAC 
CTTGAACAGC CGTCATACAG GTATGAGGCC TCAAGCTACC CAGACCAGTT TTCTCGGAAC 
TATGACCATC GCCTACGGTT TGAAGATCGA ATCCCTACCT ATGAAGACCA GTGGTCATAT 
TATGATGACA AACAGCCCTA CCAACCTCGG CCTTTTGAGA ATCAGCATCC CCGAGACCTG 
GACTCCAGAC AACATCCNGA GGAGGCTTCA GAACGAGGTT ATNTCCAGCG TTTTGAAGAG 
CCAGCCCCTC TGTCATACGA CAGTAGAACA CGCTATGAGC AGCTGCCTCG AACCTCTACT 
CTACGACATG AAGAGCAGCC AGCCCCTGCA TATGAGGTGC ACAACAGGTA CAGGCCAGAG 
GCACAGCCCT ATTCTTCAAC AGGTCCTAAG TCATCTGAGC CCAAGCAGTA CTTTGACCAG 
TACCCGCGAA GTTATGAGCA AGTACCACCA CCAGGCTTTA CCTCCAAAAC AGGCCATTAC 
GAGCCTCTCC ATGGTGCTGC AGTTGTCCCT CCTCTGATAC CTTCCTCTCA ACATAAGCCA 
GAAGTCCTGC CCTCGGCTAC CAAACCACAG CCTCCACCCC CAACCCTAAC TGAGGAGGAG 
GAGGATCCAG CAATGAAACC ACAGTCCGTG CTCANCAGAG TCAAAATGTT TGAAAACAAA 
AGATCTGCGT CTTTGGAGAA CAAGAAAGAT GTGAATGACA CTGCCAGCTT TAAGCCTCCA 
GAAGTAGCAT CTAAACCTCC AAGTGCTTCT CTTGCTGGCC CTAAACCTGT TCCTCAGAGT 
CAGTTTAGTG AGCANGACAA AACACTCTAC AGGCTCCCAG AGCCTCAGAA ACCTCAAGTG 
AAGCCACCCG ANGATATTGT TCGATCAAAT CATTACGACC CTGAAGAGGA TGAAGAATAT 
TACAGGAAAC AGCTCTCTTA CTTTGANCGA AGAAGTTTTG AGAGCAAGCC TTCTGCACAT 
CTTCCTGCTG GCCATCACTC AGAGCCTGCT AAGCCAGTCC ATTCTCAGAG CCAGCCCAAC 
TTTTCTAGTT ACTCTTCAAA GGGAAAACCC GAAACTGATG CTGTGGATAG ATCATTCAGT 
GAGAAACGTT ATGATCCAGC CCAGGCCACG CCTCCTCCTC CTCCGTTGCC CTCACAGTAC 
AGCCAGCCAG CTCCCCCTCT GTCCAGCTCT TCTCTCCACA TACATTCCAA GGGCGCCCAG 
GGCGAAGGCA ATTCAGTATC ATTGGATTTT CAGAACTCAT ATATGTCCAA ACCAGACCCA 
CCCCCATCTC AGAGTAAACC AGCAACTTTC AGACCACCAA CCCGAGAAGA CCCTCCTCAG 
ACTTTCTATC CCCAGAAANG TTTCCCAGAC AAAGCTCCAG TTAANGGAGC TGAGCAGACT 
CAGAAAACCA TCACTCCAGT GTACAATCGA TTCACACCAA AGCCGTACAC AAGTTCTGCC 
CGGCCGTTTG AACGCAAATT TGAAAGTCCG AAGTTCAACC ATAATCTTCT GCCAAGTGAA 
ACTGTACATA AACCTGAATT GTCTTCAAAA ACTCCCACTT CTCCAAAAAC TCTTATGAAA 
GCTCATAGTT CAACACAGCC TCCAGAGTTT GACAGTGGAG TTGAAACTTT CTCTGTTCAC 
ACAGATNAGC CTAAATATCA AATGAATAAT ATCAGCACCA TGCCTAAAGC TGTCCCTGTG 
AGTCCTTCAG CTGTGGAAGA AGATGAAGAT GAGGATGGTC ATACTGTAGT GGCTACAGCT 
CGTGGCATTT TTAACAGCAA TGGTGGTGTG TTGAGTTCCA TAGAAACCGG TGTTAGTATA 
ATTATCCCAC AAGGAGCCAT TCCTGAAGGA ATTGAACAAG AAATCTATTT CAAAGTCTGC 
AGAGACAATA GCATTCTCCC ACCTTTAGAT AAAGAGAAAG GTGAAACTCT GCTAAGTCCC 
CTAGTGATGT GTGGGCCCCA TGGCCTCAAG TTCCTGAAGC CCGTGGAGCT GCGCTTGCCA 
CACTGTGACC CTAAAACCTG GCAAAACAAG TGTCTTCCTG GAGATCCGAA TTACCTTGTT 
GGAGCCAACT GTGTTTCTGT CCTGATTGAC CACTTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_029334980.1
CDS	68..5266
Translation	MKYQKYLTVLQMAIGVTPSNRGSLLPLKRKLWVTPSSENPHGATSNVSQGKPSLRRIKGRLHRSKSLDSIDFCELTSTAMEETAIWEQHTVTLHRAPGFGFGIAISGGRDNPHFQSGETSIVISDVLKGGPAEGQLQENDRVAMVNGVSMDNVEHAFAVQQLRKSGKNAKITIRRKKKVQIPVSHPDPEPVSDNEDDSYDEEVHDPRAGRGALANRRSEKSWARDRSASRERSLSPRSDRRSVASSQPAKPTKVTLVKSRKNEEYGLRLASHIFVKEISQDSLAARDGNIQEGDVVLKINGTVTENMSLTDAKTLIERSKGKLKMVVQRDERATLLNVPDLSDSIHSANASERDDISEIQSLASDHSGRSHDRPPRRSQSRSPDQRSEPSDHSTQSPQQPSNGSLRSREQERMSKPGAISTPVKHADDHTPKAVEEVTVEKNEKQTPTLPEPKPVYAQVGQPDVDLPVSPSDGVLPNSTHEDGILRPSMKLVKFRKGDSVGLRLAGGNDVGIFVAGVLEDSPAAKEGLEEGDQILRVNNVDFTNIIREEAVLFLLDLPKGEEVTILAQKKKGVYRRIVESDVGDSFYIRTHFEYEKESPYGLSFNKGEVFRVVDTLYNGKLGSWLAIRIGKNHKEVERGIIPNKNRAEQLASVQYTLPKTAGGDRADFWRFRGLRSSKRNLRKSREDLSAQPVQTKFPAYERVVLREAGFLRPVTIFGPIADVAREKLAREEPDIYQIAKSEPRDAGTDHRSSGIIRLHTIKQIIDQDKHALLDVTPNAVDRLNYAQWYPIVVFLNPDSKQGVKTMRMRLCPESRKSARKLYERSHKLRKNNHHLFTTTINLNSMNDGWYGALKEAIQQQQNQLVWVSEGKADGATSDDLDLHDDRLSYLSAPGSEYSMYSTDSRHTSDYEDTDTEGGAYTDQELDETLNDEVGTPPESAITnullSSEPVREDSSGMHHENQTYPPYSPQAQPQAIHRLDSPGLKPASQQVYRKEPYPEEMMRQNHILKQPALGHPGQRPDKEPNLAYEPQLPYnullEKQASRDLEQPSYRYEASSYPDQFSRNYDHRLRFEDRIPTYEDQWSYYDDKQPYQPRPFENQHPRDLDSRQHnullEEASERGYnullQRFEEPAPLSYDSRTRYEQLPRTSTLRHEEQPAPAYEVHNRYRPEAQPYSSTGPKSSEPKQYFDQYPRSYEQVPPPGFTSKTGHYEPLHGAAVVPPLIPSSQHKPEVLPSATKPQPPPPTLTEEEEDPAMKPQSVLnullRVKMFENKRSASLENKKDVNDTASFKPPEVASKPPSASLAGPKPVPQSQFSEnullDKTLYRLPEPQKPQVKPPnullDIVRSNHYDPEEDEEYYRKQLSYFnullRRSFESKPSAHLPAGHHSEPAKPVHSQSQPNFSSYSSKGKPETDAVDRSFSEKRYDPAQATPPPPPLPSQYSQPAPPLSSSSLHIHSKGAQGEGNSVSLDFQNSYMSKPDPPPSQSKPATFRPPTREDPPQTFYPQKnullFPDKAPVnullGAEQTQKTITPVYNRFTPKPYTSSARPFERKFESPKFNHNLLPSETVHKPELSSKTPTSPKTLMKAHSSTQPPEFDSGVETFSVHTDnullPKYQMNNISTMPKAVPVSPSAVEEDEDEDGHTVVATARGIFNSNGGVLSSIETGVSIIIPQGAIPEGIEQEIYFKVCRDNSILPPLDKEKGETLLSPLVMCGPHGLKFLKPVELRLPHCDPKTWQNKCLPGDPNYLVGANCVSVLIDHF

Target ORF information:

RefSeq Version	XM_029479120.1
Organism	Mus caroli(Ryukyu mouse)
Definition	Mus caroli tight junction protein 1 (Tjp1), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_029479120.1

ORF Insert Sequence:

ATGAAGTACC AGAAATACCT GACGGTGCTG CAGATGGCCA TCGGCGTCAC CCCGTCCAAC 
CGCGGCAGCC TCCTGCCGCT CAAGAGGAAG CTGTGGGTAA CTCCATCCTC TGAAAACCCG 
CATGGTGCTA CTTCCAATGT CAGCCAAGGA AAACCTTCTC TAAGACGAAT TAAAGGGAGA 
CTACATCGAA GCAAAAGCCT TGACAGTATC GACTTCTGTG AACTCACTAG CACAGCAATG 
GAGGAAACGG CTATATGGGA ACAGCACACA GTGACGCTTC ACAGGGCTCC TGGGTTTGGA 
TTTGGAATTG CAATCTCTGG TGGAAGAGAT AATCCTCATT TTCAGAGTGG GGAAACCTCC 
ATAGTGATTT CTGATGTGCT AAAAGGAGGG CCAGCTGAAG GACAGCTACA GGAAAATGAC 
CGAGTTGCAA TGGTTAATGG AGTTTCAATG GATAACGTTG AGCATGCTTT TGCTGTTCAG 
CAGCTAAGGA AAAGTGGGAA AAACGCAAAA ATTACTATCC GAAGGAAGAA GAAAGTTCAG 
ATCCCTGTAA GTCACCCAGA TCCTGAGCCG GTGTCTGATA ATGAAGATGA TAGCTACGAC 
GAAGAAGTAC ATGACCCAAG AGCTGGCCGC GGTGCTTTAG CGAACAGAAG GAGCGAGAAG 
AGCTGGGCAA GGGATAGGAG TGCAAGCAGG GAGAGGAGCC TGTCCCCTCG CTCGGACAGG 
CGGTCGGTGG CCTCCAGTCA GCCCGCAAAA CCCACCAAGG TCACACTGGT GAAGTCTCGG 
AAAAATGAAG AATATGGTCT TCGATTGGCC AGCCATATAT TTGTAAAGGA AATTTCACAA 
GATAGTTTGG CAGCAAGAGA TGGTAACATT CAAGAAGGGG ATGTTGTCTT GAAGATAAAT 
GGTACTGTGA CAGAAAATAT GTCATTGACA GATGCAAAAA CACTGATAGA AAGGTCTAAA 
GGCAAGTTAA AAATGGTAGT GCAAAGAGAT GAGCGGGCTA CCTTACTGAA CGTCCCTGAC 
CTTTCGGATA GTATCCATTC TGCTAATGCC TCGGAAAGAG ATGACATTTC AGAAATTCAG 
TCACTAGCAT CAGACCATTC AGGTCGCTCG CATGACAGGC CACCCCGCCG CAGCCAGTCA 
CGATCTCCTG ACCAACGTTC AGAGCCCTCC GATCATTCCA CACAGTCTCC ACAGCAGCCC 
AGCAATGGCA GTCTCCGGAG CAGAGAGCAA GAGCGAATGT CTAAACCTGG GGCCATCTCA 
ACTCCTGTAA AACATGCAGA TGATCATACA CCCAAAGCAG TGGAAGAAGT TACAGTTGAG 
AAAAATGAGA AGCAGACACC CACTCTTCCA GAACCGAAGC CTGTATATGC TCAAGTTGGA 
CAACCAGATG TGGATTTACC CGTCAGCCCT TCTGATGGTG TTCTGCCTAA TTCAACTCAT 
GAAGATGGGA TACTTAGGCC CAGCATGAAA CTGGTAAAAT TCAGAAAAGG AGATAGTGTG 
GGTTTGCGAC TAGCTGGTGG AAATGATGTC GGAATATTTG TAGCTGGCGT TCTAGAAGAT 
AGCCCTGCAG CCAAAGAAGG CTTAGAGGAA GGTGATCAAA TTCTCAGGGT GAACAATGTA 
GATTTCACAA ATATCATAAG AGAAGAGGCT GTCCTTTTCC TCCTTGACCT CCCTAAAGGT 
GAAGAAGTGA CCATCCTGGC TCAGAAGAAG AAGGGTGTTT ATCGCCGCAT TGTAGAATCA 
GATGTAGGAG ATTCATTCTA TATTAGAACG CATTTTGAGT ATGAAAAAGA ATCTCCTTAC 
GGACTTAGTT TTAACAAAGG AGAGGTGTTC CGGGTCGTGG ATACTTTATA CAATGGAAAG 
CTGGGCTCTT GGCTTGCCAT TCGAATTGGC AAAAATCATA AGGAGGTAGA ACGAGGCATC 
ATCCCTAATA AGAACAGAGC TGAGCAGTTA GCCAGTGTAC AGTACACACT TCCAAAGACA 
GCGGGTGGTG ATCGGGCAGA CTTCTGGAGG TTTCGAGGTC TTCGCAGCTC CAAGAGAAAT 
CTTCGAAAAA GCAGAGAGGA CTTGTCAGCT CAGCCAGTTC AAACTAAGTT CCCAGCTTAT 
GAAAGGGTTG TTCTTCGAGA AGCTGGATTC CTAAGACCTG TAACCATCTT TGGACCAATA 
GCTGATGTTG CCAGAGAAAA GTTGGCAAGA GAGGAACCAG ATATCTATCA GATTGCAAAA 
AGTGAACCAC GAGATGCTGG GACTGACCAT CGTAGCTCTG GCATCATTCG CCTTCATACA 
ATAAAGCAAA TCATAGATCA GGATAAACAT GCGTTATTAG ATGTAACGCC AAATGCGGTT 
GATCGTCTTA ATTATGCGCA GTGGTATCCA ATTGTTGTGT TCCTTAACCC TGACTCTAAG 
CAAGGTGTAA AAACAATGAG GATGAGGTTA TGTCCGGAGT CTCGGAAAAG CGCCAGGAAG 
CTATATGAAC GCTCTCATAA GCTTCGCAAA AACAATCACC ACCTTTTCAC AACTACAATT 
AACTTAAACT CAATGAATGA TGGTTGGTAC GGTGCCCTGA AAGAAGCCAT TCAGCAGCAA 
CAGAACCAGC TGGTGTGGGT CTCTGAGGGG AAGGCGGATG GTGCTACAAG TGATGACCTT 
GATTTGCATG ATGATCGTCT GTCCTACCTG TCAGCCCCAG GTAGTGAGTA CTCAATGTAT 
AGCACGGACA GTAGACACAC TTCTGACTAT GAAGACACAG ATACAGAAGG CGGGGCCTAC 
ACTGATCAAG AACTAGATGA AACTCTTAAT GATGAGGTGG GGACTCCCCC GGAGTCTGCC 
ATTACACNGT CCTCTGAGCC TGTAAGAGAG GATTCCTCTG GGATGCATCA TGAAAACCAG 
ACCTATCCTC CTTACTCACC ACAAGCGCAG CCACAAGCTA TTCATAGACT AGACTCCCCT 
GGACTTAAGC CAGCCTCTCA ACAGGTGTAT AGAAAGGAGC CGTATCCTGA AGAAATGATG 
AGACAAAACC ATATTTTAAA ACAACCAGCT CTTGGTCACC CAGGGCAGAG GCCAGATAAA 
GAGCCAAATC TAGCCTATGA ACCCCAACTT CCATATANAG AAAAACAAGC CAGCAGAGAC 
CTTGAACAGC CGTCATACAG GTATGAGGCC TCAAGCTACC CAGACCAGTT TTCTCGGAAC 
TATGACCATC GCCTACGGTT TGAAGATCGA ATCCCTACCT ATGAAGACCA GTGGTCATAT 
TATGATGACA AACAGCCCTA CCAACCTCGG CCTTTTGAGA ATCAGCATCC CCGAGACCTG 
GACTCCAGAC AACATCCNGA GGAGGCTTCA GAACGAGGTT ATNTCCAGCG TTTTGAAGAG 
CCAGCCCCTC TGTCATACGA CAGTAGAACA CGCTATGAGC AGCTGCCTCG AACCTCTACT 
CTACGACATG AAGAGCAGCC AGCCCCTGCA TATGAGGTGC ACAACAGGTA CAGGCCAGAG 
GCACAGCCCT ATTCTTCAAC AGGTCCTAAG TCATCTGAGC CCAAGCAGTA CTTTGACCAG 
TACCCGCGAA GTTATGAGCA AGTACCACCA CCAGGCTTTA CCTCCAAAAC AGGCCATTAC 
GAGCCTCTCC ATGGTGCTGC AGTTGTCCCT CCTCTGATAC CTTCCTCTCA ACATAAGCCA 
GAAGTCCTGC CCTCGGCTAC CAAACCACAG CCTCCACCCC CAACCCTAAC TGAGGAGGAG 
GAGGATCCAG CAATGAAACC ACAGTCCGTG CTCANCAGAG TCAAAATGTT TGAAAACAAA 
AGATCTGCGT CTTTGGAGAA CAAGAAAGAT GTGAATGACA CTGCCAGCTT TAAGCCTCCA 
GAAGTAGCAT CTAAACCTCC AAGTGCTTCT CTTGCTGGCC CTAAACCTGT TCCTCAGAGT 
CAGTTTAGTG AGCANGACAA AACACTCTAC AGGCTCCCAG AGCCTCAGAA ACCTCAAGTG 
AAGCCACCCG ANGATATTGT TCGATCAAAT CATTACGACC CTGAAGAGGA TGAAGAATAT 
TACAGGAAAC AGCTCTCTTA CTTTGANCGA AGAAGTTTTG AGAGCAAGCC TTCTGCACAT 
CTTCCTGCTG GCCATCACTC AGAGCCTGCT AAGCCAGTCC ATTCTCAGAG CCAGCCCAAC 
TTTTCTAGTT ACTCTTCAAA GGGAAAACCC GAAACTGATG CTGTGGATAG ATCATTCAGT 
GAGAAACGTT ATGATCCAGC CCAGGCCACG CCTCCTCCTC CTCCGTTGCC CTCACAGTAC 
AGCCAGCCAG CTCCCCCTCT GTCCAGCTCT TCTCTCCACA TACATTCCAA GGGCGCCCAG 
GGCGAAGGCA ATTCAGTATC ATTGGATTTT CAGAACTCAT ATATGTCCAA ACCAGACCCA 
CCCCCATCTC AGAGTAAACC AGCAACTTTC AGACCACCAA CCCGAGAAGA CCCTCCTCAG 
ACTTTCTATC CCCAGAAANG TTTCCCAGAC AAAGCTCCAG TTAANGGAGC TGAGCAGACT 
CAGAAAACCA TCACTCCAGT GTACAATCGA TTCACACCAA AGCCGTACAC AAGTTCTGCC 
CGGCCGTTTG AACGCAAATT TGAAAGTCCG AAGTTCAACC ATAATCTTCT GCCAAGTGAA 
ACTGTACATA AACCTGAATT GTCTTCAAAA ACTCCCACTT CTCCAAAAAC TCTTATGAAA 
GCTCATAGTT CAACACAGCC TCCAGAGTTT GACAGTGGAG TTGAAACTTT CTCTGTTCAC 
ACAGATNAGC CTAAATATCA AATGAATAAT ATCAGCACCA TGCCTAAAGC TGTCCCTGTG 
AGTCCTTCAG CTGTGGAAGA AGATGAAGAT GAGGATGGTC ATACTGTAGT GGCTACAGCT 
CGTGGCATTT TTAACAGCAA TGGTGGTGTG TTGAGTTCCA TAGAAACCGG TGTTAGTATA 
ATTATCCCAC AAGGAGCCAT TCCTGAAGGA ATTGAACAAG AAATCTATTT CAAAGTCTGC 
AGAGACAATA GCATTCTCCC ACCTTTAGAT AAAGAGAAAG GTGAAACTCT GCTAAGTCCC 
CTAGTGATGT GTGGGCCCCA TGGCCTCAAG TTCCTGAAGC CCGTGGAGCT GCGCTTGCCA 
CACTGTGACC CTAAAACCTG GCAAAACAAG TGTCTTCCTG GAGATCCGAA TTACCTTGTT 
GGAGCCAACT GTGTTTCTGT CCTGATTGAC CACTTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Tjp1 ( XM_029479121.1 ) cDNA ORF clone, Mus caroli(Ryukyu mouse) -> XP_029334981.1 Mus caroli tight junction protein 1 (Tjp1), transcript variant X7, mRNA.

Price & Availability↑

CloneID	OMv74618
Clone ID Related Accession (Same CDS sequence)	XM_029479121.1
Accession Version	XM_029479121.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5259bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-06-09
Organism	Mus caroli(Ryukyu mouse)
Product	tight junction protein ZO-1 isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_034576.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus caroli Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCACCA GGGCCGCGGC CGCTAAGAGC ACAGCAATGG AGGAAACGGC TATATGGGAA 
CAGCACACAG TGATGCTTCA CAGGGCTCCT GGGTTTGGAT TTGGAATTGC AATCTCTGGT 
GGAAGAGATA ATCCTCATTT TCAGAGTGGG GAAACCTCCA TAGTGATTTC TGATGTGCTA 
AAAGGAGGGC CAGCTGAAGG ACAGCTACAG GAAAATGACC GAGTTGCAAT GGTTAATGGA 
GTTTCAATGG ATAACGTTGA ACATGCTTTT GCTGTTCAGC AGCTAAGGAA AAGTGGGAAA 
AACGCAAAAA TTACTATGCG AAGGAAGAAG AAAGTTCAGA TCCCTGTAAG TCACCCAGAT 
CCTGAGCCGG TGTCTGATAA TGAAGATGAT AGCTACGACG AAGAAGTACA TGATCCAAGA 
GCTGGCCGCA GTGCTTTAGC GAACAGAAGG AGCGAGAAGA GCTGGGCAAG GGATAGGAGT 
GCAAGCAGGG AGAGGAGCCT GTCCCCTCGC TCGGACAGGC GGTCGGTGGC CTCCAGTCAG 
CCCGCAAAAC CCACCAAGGT CGCACTGGTG AAGTCTCGGA AAAATGAAGA ATATGGTCTT 
CGATTGGCCA GCCATATATT TGTAAAGGAA ATTTCACAAG ATAGTTTGGC AGCAAGAGAT 
GGTAACATTC AAGAAGGGGA TGTTGTCTTG AAGATAAATG GTACTGTGAC AGAAAATATG 
TCATTGACAG ATGCAAAAAC ACTGATAGAA AGGTCTAAAG GCAAGTTAAA AATGGTAGTG 
CAAAGAGATG AGCGGGCTAC CTTACTGAAC GTCCCTGACC TTTCGGATAG TATCCATTCT 
GCTAATGCCT CGGAAAGAGA TGACATTTCA GAAATTCAGT CACTAGCATC AGACCATTCA 
GGTCGCTCGC ATGACAGGCC ACCCCGCCGC AGCCAGTCAC GATCTCCTGA CCAACGTTCA 
GAGCCCTCCG ATCATTCCAC ACAGTCTCCA CAGCAGCCCA GCAATGGCAG TCTCCGGAGC 
AGAGAGCAAG AGCGAATGTC TAAACCTGGG GCCATCTCAA CTCCTGTAAA ACATGCAGAT 
GATCATACAC CCAAAGCAGT GGAAGAAGTT ACAGTTGAGA AAAATGAGAA GCAGACACCC 
ACTCTTCCAG AACCGAAGCC TGTATATGCT CAAGTTGGAC AACCAGATGT GGATTTACCC 
GTCAGCCCTT CTGATGGTGT TCTGCCTAAT TCAACTCATG AAGATGGGAT ACTTAGGCCC 
AGCATGAAAC TGGTAAAATT CAGAAAAGGA GATAGTGTGG GTTTGCGACT AGCTGGTGGA 
AATGATGTCG GAATATTTGT AGCTGGCGTT CTAGAAGATA GCCCTGCAGC CAAAGAAGGC 
TTAGAGGAAG GTGATCAAAT TCTCAGGGTG AACAATGTAG ATTTCACAAA TATCATAAGA 
GAAGAGGCTG TCCTTTTCCT CCTTGACCTC CCTAAAGGTG AAGAAGTGAC CATCCTGGCT 
CAGAAGAAGA AGGGTGTTTA TCGCCGCATT GTAGAATCAG ATGTAGGAGA TTCATTCTAT 
ATTAGAACGC ATTTTGAGTA TGAAAAAGAA TCTCCTTACG GACTTAGTTT TAACAAAGGA 
GAGGTGTTCC GGGTCGTGGA TACTTTATAC AATGGAAAGC TGGGCTCTTG GCTTGCCATT 
CGAATTGGCA AAAATCATAA GGAGGTAGAA CGAGGCATCA TCCCTAATAA GAACAGAGCT 
GAGCAGTTAG CCAGTGTACA GTACACACTT CCAAAGACAG CGGGTGGTGA TCGGGCAGAC 
TTCTGGAGGT TTCGAGGTCT TCGCAGCTCC AAGAGAAATC TTCGAAAAAG CAGAGAGGAC 
TTGTCAGCTC AGCCAGTTCA AACTAAGTTC CCAGCTTATG AAAGGGTTGT TCTTCGAGAA 
GCTGGATTCC TAAGACCTGT AACCATCTTT GGACCAATAG CTGATGTTGC CAGAGAAAAG 
TTGGCAAGAG AGGAACCAGA TATCTATCAG ATTGCAAAAA GTGAACCACG AGATGCTGGG 
ACTGACCATC GTAGCTCTGG CATCATTCGC CTTCATACAA TAAAGCAAAT CATAGATCAG 
GATAAACATG CGTTATTAGA TGTAACGCCA AATGCGGTTG ATCGTCTTAA TTATGCGCAG 
TGGTATCCAA TTGTTGTGTT CCTTAACCCT GACTCTAAGC AAGGTGTAAA AACAATGAGG 
ATGAGGTTAT GTCCGGAGTC TCGGAAAAGC GCCAGGAAGC TATATGAACG CTCTCATAAG 
CTTCGCAAAA ACAATCACCA CCTTTTCACA ACTACAATTA ACTTAAACTC AATGAATGAT 
GGTTGGTACG GTGCCCTGAA AGAAGCCATT CAGCAGCAAC AGAACCAGCT GGTGTGGGTC 
TCTGAGGGGA AGGCGGATGG TGCTACAAGT GATGACCTTG ATTTGCATGA TGATCGTCTG 
TCCTACCTGT CAGCCCCAGG TAGTGAGTAC TCAATGTATA GCACGGACAG TAGACACACT 
TCTGACTATG AAGACACAGA TACAGAAGGC GGGGCCTACA CTGATCAAGA ACTAGATGAA 
ACTCTTAATG ATGAGGTGGG GACTCCCCCG GAGTCTGCCA TTACACNGTC CTCTGAGCCT 
GTAAGAGAGG ATTCCTCTGG GATGCATCAT GAAAACCAGA CCTATCCTCC TTACTCACCA 
CAAGCGCAGC CACAAGCTAT TCATAGACTA GACTCCCCTG GACTTAAGCC AGCCTCTCAA 
CAGAAAGCAG AAGCCTCATC TCCAGTCCCT TACCTTTCGC CTGAAACAAA CCCAGCATCG 
TCAGCCTCTG CAGTTAATCC TAATGTCAGT GTAACTAATG TCAGCCTGGA GGAGCCTGCC 
CCAGCCCCTC CCGCCTCGCA CGCATCCCAG GCTGGTGGTT TAGGAGCACC AGGTGCTGAG 
GCAGCTCACG TAGTGCTCAG AGGTGAAGGA CCACCATTGC CGCCGCATGC AGACCCAGCA 
CAGGTGTATA GAAAGGAGCC GTATCCTGAA GAAATGATGA GACAAAACCA TATTTTAAAA 
CAACCAGCTC TTGGTCACCC AGGGCAGAGG CCAGATAAAG AGCCAAATCT AGCCTATGAA 
CCCCAACTTC CATATANAGA AAAACAAGCC AGCAGAGACC TTGAACAGCC GTCATACAGG 
TATGAGGCCT CAAGCTACCC AGACCAGTTT TCTCGGAACT ATGACCATCG CCTACGGTTT 
GAAGATCGAA TCCCTACCTA TGAAGACCAG TGGTCATATT ATGATGACAA ACAGCCCTAC 
CAACCTCGGC CTTTTGAGAA TCAGCATCCC CGAGACCTGG ACTCCAGACA ACATCCNGAG 
GAGGCTTCAG AACGAGGTTA TNTCCAGCGT TTTGAAGAGC CAGCCCCTCT GTCATACGAC 
AGTAGAACAC GCTATGAGCA GCTGCCTCGA ACCTCTACTC TACGACATGA AGAGCAGCCA 
GCCCCTGCAT ATGAGGTGCA CAACAGGTAC AGGCCAGAGG CACAGCCCTA TTCTTCAACA 
GGTCCTAAGT CATCTGAGCC CAAGCAGTAC TTTGACCAGT ACCCGCGAAG TTATGAGCAA 
GTACCACCAC CAGGCTTTAC CTCCAAAACA GGCCATTACG AGCCTCTCCA TGGTGCTGCA 
GTTGTCCCTC CTCTGATACC TTCCTCTCAA CATAAGCCAG AAGTCCTGCC CTCGGCTACC 
AAACCACAGC CTCCACCCCC AACCCTAACT GAGGAGGAGG AGGATCCAGC AATGAAACCA 
CAGTCCGTGC TCANCAGAGT CAAAATGTTT GAAAACAAAA GATCTGCGTC TTTGGAGAAC 
AAGAAAGATG TGAATGACAC TGCCAGCTTT AAGCCTCCAG AAGTAGCATC TAAACCTCCA 
AGTGCTTCTC TTGCTGGCCC TAAACCTGTT CCTCAGAGTC AGTTTAGTGA GCANGACAAA 
ACACTCTACA GGCTCCCAGA GCCTCAGAAA CCTCAAGTGA AGCCACCCGA NGATATTGTT 
CGATCAAATC ATTACGACCC TGAAGAGGAT GAAGAATATT ACAGGAAACA GCTCTCTTAC 
TTTGANCGAA GAAGTTTTGA GAGCAAGCCT TCTGCACATC TTCCTGCTGG CCATCACTCA 
GAGCCTGCTA AGCCAGTCCA TTCTCAGAGC CAGCCCAACT TTTCTAGTTA CTCTTCAAAG 
GGAAAACCCG AAACTGATGC TGTGGATAGA TCATTCAGTG AGAAACGTTA TGATCCAGCC 
CAGGCCACGC CTCCTCCTCC TCCGTTGCCC TCACAGTACA GCCAGCCAGC TCCCCCTCTG 
TCCAGCTCTT CTCTCCACAT ACATTCCAAG GGCGCCCAGG GCGAAGGCAA TTCAGTATCA 
TTGGATTTTC AGAACTCATA TATGTCCAAA CCAGACCCAC CCCCATCTCA GAGTAAACCA 
GCAACTTTCA GACCACCAAC CCGAGAAGAC CCTCCTCAGA CTTTCTATCC CCAGAAANGT 
TTCCCAGACA AAGCTCCAGT TAANGGAGCT GAGCAGACTC AGAAAACCAT CACTCCAGTG 
TACAATCGAT TCACACCAAA GCCGTACACA AGTTCTGCCC GGCCGTTTGA ACGCAAATTT 
GAAAGTCCGA AGTTCAACCA TAATCTTCTG CCAAGTGAAA CTGTACATAA ACCTGAATTG 
TCTTCAAAAA CTCCCACTTC TCCAAAAACT CTTATGAAAG CTCATAGTTC AACACAGCCT 
CCAGAGTTTG ACAGTGGAGT TGAAACTTTC TCTGTTCACA CAGATNAGCC TAAATATCAA 
ATGAATAATA TCAGCACCAT GCCTAAAGCT GTCCCTGTGA GTCCTTCAGC TGTGGAAGAA 
GATGAAGATG AGGATGGTCA TACTGTAGTG GCTACAGCTC GTGGCATTTT TAACAGCAAT 
GGTGGTGTGT TGAGTTCCAT AGAAACCGGT GTTAGTATAA TTATCCCACA AGGAGCCATT 
CCTGAAGGAA TTGAACAAGA AATCTATTTC AAAGTCTGCA GAGACAATAG CATTCTCCCA 
CCTTTAGATA AAGAGAAAGG TGAAACTCTG CTAAGTCCCC TAGTGATGTG TGGGCCCCAT 
GGCCTCAAGT TCCTGAAGCC CGTGGAGCTG CGCTTGCCAC ACTGTGCGTC CATGACTCCT 
GACGGTGACC CTAAAACCTG GCAAAACAAG TGTCTTCCTG GAGATCCGAA TTACCTTGTT 
GGAGCCAACT GTGTTTCTGT CCTGATTGAC CACTTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_029334981.1
CDS	65..5323
Translation	MSTRAAAAKSTAMEETAIWEQHTVMLHRAPGFGFGIAISGGRDNPHFQSGETSIVISDVLKGGPAEGQLQENDRVAMVNGVSMDNVEHAFAVQQLRKSGKNAKITMRRKKKVQIPVSHPDPEPVSDNEDDSYDEEVHDPRAGRSALANRRSEKSWARDRSASRERSLSPRSDRRSVASSQPAKPTKVALVKSRKNEEYGLRLASHIFVKEISQDSLAARDGNIQEGDVVLKINGTVTENMSLTDAKTLIERSKGKLKMVVQRDERATLLNVPDLSDSIHSANASERDDISEIQSLASDHSGRSHDRPPRRSQSRSPDQRSEPSDHSTQSPQQPSNGSLRSREQERMSKPGAISTPVKHADDHTPKAVEEVTVEKNEKQTPTLPEPKPVYAQVGQPDVDLPVSPSDGVLPNSTHEDGILRPSMKLVKFRKGDSVGLRLAGGNDVGIFVAGVLEDSPAAKEGLEEGDQILRVNNVDFTNIIREEAVLFLLDLPKGEEVTILAQKKKGVYRRIVESDVGDSFYIRTHFEYEKESPYGLSFNKGEVFRVVDTLYNGKLGSWLAIRIGKNHKEVERGIIPNKNRAEQLASVQYTLPKTAGGDRADFWRFRGLRSSKRNLRKSREDLSAQPVQTKFPAYERVVLREAGFLRPVTIFGPIADVAREKLAREEPDIYQIAKSEPRDAGTDHRSSGIIRLHTIKQIIDQDKHALLDVTPNAVDRLNYAQWYPIVVFLNPDSKQGVKTMRMRLCPESRKSARKLYERSHKLRKNNHHLFTTTINLNSMNDGWYGALKEAIQQQQNQLVWVSEGKADGATSDDLDLHDDRLSYLSAPGSEYSMYSTDSRHTSDYEDTDTEGGAYTDQELDETLNDEVGTPPESAITnullSSEPVREDSSGMHHENQTYPPYSPQAQPQAIHRLDSPGLKPASQQKAEASSPVPYLSPETNPASSASAVNPNVSVTNVSLEEPAPAPPASHASQAGGLGAPGAEAAHVVLRGEGPPLPPHADPAQVYRKEPYPEEMMRQNHILKQPALGHPGQRPDKEPNLAYEPQLPYnullEKQASRDLEQPSYRYEASSYPDQFSRNYDHRLRFEDRIPTYEDQWSYYDDKQPYQPRPFENQHPRDLDSRQHnullEEASERGYnullQRFEEPAPLSYDSRTRYEQLPRTSTLRHEEQPAPAYEVHNRYRPEAQPYSSTGPKSSEPKQYFDQYPRSYEQVPPPGFTSKTGHYEPLHGAAVVPPLIPSSQHKPEVLPSATKPQPPPPTLTEEEEDPAMKPQSVLnullRVKMFENKRSASLENKKDVNDTASFKPPEVASKPPSASLAGPKPVPQSQFSEnullDKTLYRLPEPQKPQVKPPnullDIVRSNHYDPEEDEEYYRKQLSYFnullRRSFESKPSAHLPAGHHSEPAKPVHSQSQPNFSSYSSKGKPETDAVDRSFSEKRYDPAQATPPPPPLPSQYSQPAPPLSSSSLHIHSKGAQGEGNSVSLDFQNSYMSKPDPPPSQSKPATFRPPTREDPPQTFYPQKnullFPDKAPVnullGAEQTQKTITPVYNRFTPKPYTSSARPFERKFESPKFNHNLLPSETVHKPELSSKTPTSPKTLMKAHSSTQPPEFDSGVETFSVHTDnullPKYQMNNISTMPKAVPVSPSAVEEDEDEDGHTVVATARGIFNSNGGVLSSIETGVSIIIPQGAIPEGIEQEIYFKVCRDNSILPPLDKEKGETLLSPLVMCGPHGLKFLKPVELRLPHCASMTPDGDPKTWQNKCLPGDPNYLVGANCVSVLIDHF

Target ORF information:

RefSeq Version	XM_029479121.1
Organism	Mus caroli(Ryukyu mouse)
Definition	Mus caroli tight junction protein 1 (Tjp1), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_029479121.1

ORF Insert Sequence:

ATGTCCACCA GGGCCGCGGC CGCTAAGAGC ACAGCAATGG AGGAAACGGC TATATGGGAA 
CAGCACACAG TGATGCTTCA CAGGGCTCCT GGGTTTGGAT TTGGAATTGC AATCTCTGGT 
GGAAGAGATA ATCCTCATTT TCAGAGTGGG GAAACCTCCA TAGTGATTTC TGATGTGCTA 
AAAGGAGGGC CAGCTGAAGG ACAGCTACAG GAAAATGACC GAGTTGCAAT GGTTAATGGA 
GTTTCAATGG ATAACGTTGA ACATGCTTTT GCTGTTCAGC AGCTAAGGAA AAGTGGGAAA 
AACGCAAAAA TTACTATGCG AAGGAAGAAG AAAGTTCAGA TCCCTGTAAG TCACCCAGAT 
CCTGAGCCGG TGTCTGATAA TGAAGATGAT AGCTACGACG AAGAAGTACA TGATCCAAGA 
GCTGGCCGCA GTGCTTTAGC GAACAGAAGG AGCGAGAAGA GCTGGGCAAG GGATAGGAGT 
GCAAGCAGGG AGAGGAGCCT GTCCCCTCGC TCGGACAGGC GGTCGGTGGC CTCCAGTCAG 
CCCGCAAAAC CCACCAAGGT CGCACTGGTG AAGTCTCGGA AAAATGAAGA ATATGGTCTT 
CGATTGGCCA GCCATATATT TGTAAAGGAA ATTTCACAAG ATAGTTTGGC AGCAAGAGAT 
GGTAACATTC AAGAAGGGGA TGTTGTCTTG AAGATAAATG GTACTGTGAC AGAAAATATG 
TCATTGACAG ATGCAAAAAC ACTGATAGAA AGGTCTAAAG GCAAGTTAAA AATGGTAGTG 
CAAAGAGATG AGCGGGCTAC CTTACTGAAC GTCCCTGACC TTTCGGATAG TATCCATTCT 
GCTAATGCCT CGGAAAGAGA TGACATTTCA GAAATTCAGT CACTAGCATC AGACCATTCA 
GGTCGCTCGC ATGACAGGCC ACCCCGCCGC AGCCAGTCAC GATCTCCTGA CCAACGTTCA 
GAGCCCTCCG ATCATTCCAC ACAGTCTCCA CAGCAGCCCA GCAATGGCAG TCTCCGGAGC 
AGAGAGCAAG AGCGAATGTC TAAACCTGGG GCCATCTCAA CTCCTGTAAA ACATGCAGAT 
GATCATACAC CCAAAGCAGT GGAAGAAGTT ACAGTTGAGA AAAATGAGAA GCAGACACCC 
ACTCTTCCAG AACCGAAGCC TGTATATGCT CAAGTTGGAC AACCAGATGT GGATTTACCC 
GTCAGCCCTT CTGATGGTGT TCTGCCTAAT TCAACTCATG AAGATGGGAT ACTTAGGCCC 
AGCATGAAAC TGGTAAAATT CAGAAAAGGA GATAGTGTGG GTTTGCGACT AGCTGGTGGA 
AATGATGTCG GAATATTTGT AGCTGGCGTT CTAGAAGATA GCCCTGCAGC CAAAGAAGGC 
TTAGAGGAAG GTGATCAAAT TCTCAGGGTG AACAATGTAG ATTTCACAAA TATCATAAGA 
GAAGAGGCTG TCCTTTTCCT CCTTGACCTC CCTAAAGGTG AAGAAGTGAC CATCCTGGCT 
CAGAAGAAGA AGGGTGTTTA TCGCCGCATT GTAGAATCAG ATGTAGGAGA TTCATTCTAT 
ATTAGAACGC ATTTTGAGTA TGAAAAAGAA TCTCCTTACG GACTTAGTTT TAACAAAGGA 
GAGGTGTTCC GGGTCGTGGA TACTTTATAC AATGGAAAGC TGGGCTCTTG GCTTGCCATT 
CGAATTGGCA AAAATCATAA GGAGGTAGAA CGAGGCATCA TCCCTAATAA GAACAGAGCT 
GAGCAGTTAG CCAGTGTACA GTACACACTT CCAAAGACAG CGGGTGGTGA TCGGGCAGAC 
TTCTGGAGGT TTCGAGGTCT TCGCAGCTCC AAGAGAAATC TTCGAAAAAG CAGAGAGGAC 
TTGTCAGCTC AGCCAGTTCA AACTAAGTTC CCAGCTTATG AAAGGGTTGT TCTTCGAGAA 
GCTGGATTCC TAAGACCTGT AACCATCTTT GGACCAATAG CTGATGTTGC CAGAGAAAAG 
TTGGCAAGAG AGGAACCAGA TATCTATCAG ATTGCAAAAA GTGAACCACG AGATGCTGGG 
ACTGACCATC GTAGCTCTGG CATCATTCGC CTTCATACAA TAAAGCAAAT CATAGATCAG 
GATAAACATG CGTTATTAGA TGTAACGCCA AATGCGGTTG ATCGTCTTAA TTATGCGCAG 
TGGTATCCAA TTGTTGTGTT CCTTAACCCT GACTCTAAGC AAGGTGTAAA AACAATGAGG 
ATGAGGTTAT GTCCGGAGTC TCGGAAAAGC GCCAGGAAGC TATATGAACG CTCTCATAAG 
CTTCGCAAAA ACAATCACCA CCTTTTCACA ACTACAATTA ACTTAAACTC AATGAATGAT 
GGTTGGTACG GTGCCCTGAA AGAAGCCATT CAGCAGCAAC AGAACCAGCT GGTGTGGGTC 
TCTGAGGGGA AGGCGGATGG TGCTACAAGT GATGACCTTG ATTTGCATGA TGATCGTCTG 
TCCTACCTGT CAGCCCCAGG TAGTGAGTAC TCAATGTATA GCACGGACAG TAGACACACT 
TCTGACTATG AAGACACAGA TACAGAAGGC GGGGCCTACA CTGATCAAGA ACTAGATGAA 
ACTCTTAATG ATGAGGTGGG GACTCCCCCG GAGTCTGCCA TTACACNGTC CTCTGAGCCT 
GTAAGAGAGG ATTCCTCTGG GATGCATCAT GAAAACCAGA CCTATCCTCC TTACTCACCA 
CAAGCGCAGC CACAAGCTAT TCATAGACTA GACTCCCCTG GACTTAAGCC AGCCTCTCAA 
CAGAAAGCAG AAGCCTCATC TCCAGTCCCT TACCTTTCGC CTGAAACAAA CCCAGCATCG 
TCAGCCTCTG CAGTTAATCC TAATGTCAGT GTAACTAATG TCAGCCTGGA GGAGCCTGCC 
CCAGCCCCTC CCGCCTCGCA CGCATCCCAG GCTGGTGGTT TAGGAGCACC AGGTGCTGAG 
GCAGCTCACG TAGTGCTCAG AGGTGAAGGA CCACCATTGC CGCCGCATGC AGACCCAGCA 
CAGGTGTATA GAAAGGAGCC GTATCCTGAA GAAATGATGA GACAAAACCA TATTTTAAAA 
CAACCAGCTC TTGGTCACCC AGGGCAGAGG CCAGATAAAG AGCCAAATCT AGCCTATGAA 
CCCCAACTTC CATATANAGA AAAACAAGCC AGCAGAGACC TTGAACAGCC GTCATACAGG 
TATGAGGCCT CAAGCTACCC AGACCAGTTT TCTCGGAACT ATGACCATCG CCTACGGTTT 
GAAGATCGAA TCCCTACCTA TGAAGACCAG TGGTCATATT ATGATGACAA ACAGCCCTAC 
CAACCTCGGC CTTTTGAGAA TCAGCATCCC CGAGACCTGG ACTCCAGACA ACATCCNGAG 
GAGGCTTCAG AACGAGGTTA TNTCCAGCGT TTTGAAGAGC CAGCCCCTCT GTCATACGAC 
AGTAGAACAC GCTATGAGCA GCTGCCTCGA ACCTCTACTC TACGACATGA AGAGCAGCCA 
GCCCCTGCAT ATGAGGTGCA CAACAGGTAC AGGCCAGAGG CACAGCCCTA TTCTTCAACA 
GGTCCTAAGT CATCTGAGCC CAAGCAGTAC TTTGACCAGT ACCCGCGAAG TTATGAGCAA 
GTACCACCAC CAGGCTTTAC CTCCAAAACA GGCCATTACG AGCCTCTCCA TGGTGCTGCA 
GTTGTCCCTC CTCTGATACC TTCCTCTCAA CATAAGCCAG AAGTCCTGCC CTCGGCTACC 
AAACCACAGC CTCCACCCCC AACCCTAACT GAGGAGGAGG AGGATCCAGC AATGAAACCA 
CAGTCCGTGC TCANCAGAGT CAAAATGTTT GAAAACAAAA GATCTGCGTC TTTGGAGAAC 
AAGAAAGATG TGAATGACAC TGCCAGCTTT AAGCCTCCAG AAGTAGCATC TAAACCTCCA 
AGTGCTTCTC TTGCTGGCCC TAAACCTGTT CCTCAGAGTC AGTTTAGTGA GCANGACAAA 
ACACTCTACA GGCTCCCAGA GCCTCAGAAA CCTCAAGTGA AGCCACCCGA NGATATTGTT 
CGATCAAATC ATTACGACCC TGAAGAGGAT GAAGAATATT ACAGGAAACA GCTCTCTTAC 
TTTGANCGAA GAAGTTTTGA GAGCAAGCCT TCTGCACATC TTCCTGCTGG CCATCACTCA 
GAGCCTGCTA AGCCAGTCCA TTCTCAGAGC CAGCCCAACT TTTCTAGTTA CTCTTCAAAG 
GGAAAACCCG AAACTGATGC TGTGGATAGA TCATTCAGTG AGAAACGTTA TGATCCAGCC 
CAGGCCACGC CTCCTCCTCC TCCGTTGCCC TCACAGTACA GCCAGCCAGC TCCCCCTCTG 
TCCAGCTCTT CTCTCCACAT ACATTCCAAG GGCGCCCAGG GCGAAGGCAA TTCAGTATCA 
TTGGATTTTC AGAACTCATA TATGTCCAAA CCAGACCCAC CCCCATCTCA GAGTAAACCA 
GCAACTTTCA GACCACCAAC CCGAGAAGAC CCTCCTCAGA CTTTCTATCC CCAGAAANGT 
TTCCCAGACA AAGCTCCAGT TAANGGAGCT GAGCAGACTC AGAAAACCAT CACTCCAGTG 
TACAATCGAT TCACACCAAA GCCGTACACA AGTTCTGCCC GGCCGTTTGA ACGCAAATTT 
GAAAGTCCGA AGTTCAACCA TAATCTTCTG CCAAGTGAAA CTGTACATAA ACCTGAATTG 
TCTTCAAAAA CTCCCACTTC TCCAAAAACT CTTATGAAAG CTCATAGTTC AACACAGCCT 
CCAGAGTTTG ACAGTGGAGT TGAAACTTTC TCTGTTCACA CAGATNAGCC TAAATATCAA 
ATGAATAATA TCAGCACCAT GCCTAAAGCT GTCCCTGTGA GTCCTTCAGC TGTGGAAGAA 
GATGAAGATG AGGATGGTCA TACTGTAGTG GCTACAGCTC GTGGCATTTT TAACAGCAAT 
GGTGGTGTGT TGAGTTCCAT AGAAACCGGT GTTAGTATAA TTATCCCACA AGGAGCCATT 
CCTGAAGGAA TTGAACAAGA AATCTATTTC AAAGTCTGCA GAGACAATAG CATTCTCCCA 
CCTTTAGATA AAGAGAAAGG TGAAACTCTG CTAAGTCCCC TAGTGATGTG TGGGCCCCAT 
GGCCTCAAGT TCCTGAAGCC CGTGGAGCTG CGCTTGCCAC ACTGTGCGTC CATGACTCCT 
GACGGTGACC CTAAAACCTG GCAAAACAAG TGTCTTCCTG GAGATCCGAA TTACCTTGTT 
GGAGCCAACT GTGTTTCTGT CCTGATTGAC CACTTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	Tjp1
Entrez Gene ID	110297425
Full Name	tight junction protein 1
Gene Type	protein-coding
Organism	Mus caroli(Ryukyu mouse)

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

Tjp1 cDNA ORF clone, Mus caroli(Ryukyu mouse)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Most Popular Services

Services & Products

見積依頼

Eメール

電話

FAX

オンラインフォーム