Pikfyve gene cDNA ORF clone, Mus caroli(Ryukyu mouse)

mRNA	Protein	Name
XM_029476064.1	XP_029331924.1	1-phosphatidylinositol 3-phosphate 5-kinase isoform X1
XM_021160793.2	XP_021016452.1	1-phosphatidylinositol 3-phosphate 5-kinase isoform X3
XM_029476096.1	XP_029331956.1	1-phosphatidylinositol 3-phosphate 5-kinase isoform X6
XM_021160800.1	XP_021016459.1	1-phosphatidylinositol 3-phosphate 5-kinase
XM_029476086.1	XP_029331946.1	1-phosphatidylinositol 3-phosphate 5-kinase isoform X4
XM_029476067.1	XP_029331927.1	1-phosphatidylinositol 3-phosphate 5-kinase isoform X1
XM_029476073.1	XP_029331933.1	1-phosphatidylinositol 3-phosphate 5-kinase isoform X2
XM_029476091.1	XP_029331951.1	1-phosphatidylinositol 3-phosphate 5-kinase isoform X5
XM_021160793.1	XP_021016452.1	1-phosphatidylinositol 3-phosphate 5-kinase
XM_021160800.2	XP_021016459.1	1-phosphatidylinositol 3-phosphate 5-kinase isoform X3
XM_029476100.1	XP_029331960.1	1-phosphatidylinositol 3-phosphate 5-kinase isoform X7

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMv66200	XM_029476100.1 Latest version!	Mus caroli phosphoinositide kinase, FYVE-type zinc finger containing (Pikfyve), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
OMv23070	XM_021160800.2 Latest version!	Mus caroli phosphoinositide kinase, FYVE-type zinc finger containing (Pikfyve), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00
OMv23070	XM_021160793.2 Latest version!	Mus caroli phosphoinositide kinase, FYVE-type zinc finger containing (Pikfyve), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00
OMv66195	XM_029476064.1 Latest version!	Mus caroli phosphoinositide kinase, FYVE-type zinc finger containing (Pikfyve), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OMv66195	XM_029476067.1 Latest version!	Mus caroli phosphoinositide kinase, FYVE-type zinc finger containing (Pikfyve), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OMv66196	XM_029476073.1 Latest version!	Mus caroli phosphoinositide kinase, FYVE-type zinc finger containing (Pikfyve), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OMv66197	XM_029476086.1 Latest version!	Mus caroli phosphoinositide kinase, FYVE-type zinc finger containing (Pikfyve), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OMv66198	XM_029476091.1 Latest version!	Mus caroli phosphoinositide kinase, FYVE-type zinc finger containing (Pikfyve), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OMv66199	XM_029476096.1 Latest version!	Mus caroli phosphoinositide kinase, FYVE-type zinc finger containing (Pikfyve), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
View Old Accession Versions >>
OMv23070	XM_021160793.1	Mus caroli phosphoinositide kinase, FYVE-type zinc finger containing (Pikfyve), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00
OMv23070	XM_021160800.1	Mus caroli phosphoinositide kinase, FYVE-type zinc finger containing (Pikfyve), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00
Hide Old Accession Versions >>

Pikfyve ( XM_029476100.1 ) cDNA ORF clone, Mus caroli(Ryukyu mouse) -> XP_029331960.1 Mus caroli phosphoinositide kinase, FYVE-type zinc finger containing (Pikfyve), transcript variant X9, mRNA.

Price & Availability↑

CloneID	OMv66200
Clone ID Related Accession (Same CDS sequence)	XM_029476100.1
Accession Version	XM_029476100.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1683bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-06-09
Organism	Mus caroli(Ryukyu mouse)
Product	1-phosphatidylinositol 3-phosphate 5-kinase isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_034570.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus caroli Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCACAG ATGACAAGAG TTCCCCGACA CTGGACTCTG CTAATGATTT GCCTCGCTCT 
CCCGCCAGTC CTTCTCACCT CACTCACTTT AAACCCTTGA CTCCTGACCA GGATGAGCCC 
CCCTTCAAGT CAGCATATAG TTCTTTTGTA AACCTTTTTC GCTTTAACAA AGAGCGGGGA 
GAAGGGGGCC AAGGAGAGCA GCAGTCTCCG AGTTCAAGTT GGGCCAGCCC TCAGATCCCT 
TCAAGAACAC AGTCTGTGAG GTCGCCTGTA CCTTATAAAA AACAGCTTAA TGAGGAGCTC 
CACCGGCGCT CTTCAGTGTT AGCAGAGAAC ACTTTGCCAC ATCCTCAGGA GAGCACAGAC 
TCCAGAAGGA AAGCAGAACC AGCCTGTGGA GGTCATGACC CACGTACAGC TGTTCAGCTT 
CGAAGCCTCA GCACAGTATT GAAACGCCTC AAAGAAATCA TGGAAGGAAA AAGCCAGGAC 
AGTGACCTGA AGCAATATTG GATGCCAGAT AGCCAGTGTA AAGAGTGCTA TGACTGCAGT 
GAAAAGTTTA CAACATTTAG GCGCAGACAC CATTGCAGAC TGTGTGGGCA GATTTTCTGC 
AGTCGTTGTT GTAATCAAGA AATCCCTGGA AAATTTATGG GCTATACAGG AGACCTCCGA 
GCATGCACCT ACTGTAGGAA AATAGCCTTA AGTTATGCTC ATTCCACAGA CAGTAATTCC 
ATTGGGGAAG ACTTGAATGC TCTTTCAGAT TCAACTTGCT CTGTGTCTAT ACTTGATCCA 
AGCGAACCTC GGACACCTGT TGGGAGTAGA AAAGCCAGTC GTAACATATT CTTAGAGGAT 
GATTTAGCCT GGCAAAGTTT GATTCATCCG GATTCCTCAA ATAGTGCTCT TTCAACAAGA 
CTCGTATCTG TTCAAGAGGA TGCTGGGAAG TCTCCTGCTC GGAACAGATC AGCCAGCATT 
ACTAATCTGT CACTGGATCG GTCTGGTTCT CCTATGGTTC CTTCATATGA GACATCTGTC 
AGTCCCCAGG CTAACCGAAA CTACATTAGG ACCGAGACGA CTGAGGATGA ACGCAAAATT 
CTTCTGGACA GTGCTCAGTT AAAGGATCTG TGGAAGAAAA TCTGCCATCA CACCAGTGGG 
ATGGAATTTC AAGATCACCG TTACTGGTTG AGAACACATC CCAACTGCAT TGTAGGAAAG 
GAATTAGTCA ACTGGCTAAT CAGAAATGGA CACATCGCTA CAAGGGCACA AGCTATAGCA 
ATTGGACAAG CAATGGTTGA TGGACGTTGG TTGGATTGTG TTAGTCATCA TGACCAGCTT 
TTCAGGGACG AATATGCGTT GTATAGACCA CTTCAGAGTA CAGAATTTTC TGAGACACCT 
TCTCCAGACA GTGACTCCGT GAACTCTGTG GAAGGACACT CCGAGCCATC CTGGTTTAAA 
GACATAAAAT TTGATGACAG TGACACAGAA CAGATTGCTG AAGAAGGTGA CGATAATTTG 
GCTAATTCTG CCAGTCCTAG CAAGCGCACC TCAGTCAGCA GTTTCCAGTC CACAGTGGAC 
AGTGACTCAG CCGCTTCTAT CAGCCTGAAC GTGGAGCTGG ACAACGTGAA CTTCCATATC 
AAGAAGCCCT CCAAGTACCC ACATGTGCCC CCTCACCCTG CTGACCAAAA AGGTAGGAGG 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_029331960.1
CDS	164..1846
Translation	MATDDKSSPTLDSANDLPRSPASPSHLTHFKPLTPDQDEPPFKSAYSSFVNLFRFNKERGEGGQGEQQSPSSSWASPQIPSRTQSVRSPVPYKKQLNEELHRRSSVLAENTLPHPQESTDSRRKAEPACGGHDPRTAVQLRSLSTVLKRLKEIMEGKSQDSDLKQYWMPDSQCKECYDCSEKFTTFRRRHHCRLCGQIFCSRCCNQEIPGKFMGYTGDLRACTYCRKIALSYAHSTDSNSIGEDLNALSDSTCSVSILDPSEPRTPVGSRKASRNIFLEDDLAWQSLIHPDSSNSALSTRLVSVQEDAGKSPARNRSASITNLSLDRSGSPMVPSYETSVSPQANRNYIRTETTEDERKILLDSAQLKDLWKKICHHTSGMEFQDHRYWLRTHPNCIVGKELVNWLIRNGHIATRAQAIAIGQAMVDGRWLDCVSHHDQLFRDEYALYRPLQSTEFSETPSPDSDSVNSVEGHSEPSWFKDIKFDDSDTEQIAEEGDDNLANSASPSKRTSVSSFQSTVDSDSAASISLNVELDNVNFHIKKPSKYPHVPPHPADQKGRR

Target ORF information:

RefSeq Version	XM_029476100.1
Organism	Mus caroli(Ryukyu mouse)
Definition	Mus caroli phosphoinositide kinase, FYVE-type zinc finger containing (Pikfyve), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_029476100.1

ORF Insert Sequence:

ATGGCCACAG ATGACAAGAG TTCCCCGACA CTGGACTCTG CTAATGATTT GCCTCGCTCT 
CCCGCCAGTC CTTCTCACCT CACTCACTTT AAACCCTTGA CTCCTGACCA GGATGAGCCC 
CCCTTCAAGT CAGCATATAG TTCTTTTGTA AACCTTTTTC GCTTTAACAA AGAGCGGGGA 
GAAGGGGGCC AAGGAGAGCA GCAGTCTCCG AGTTCAAGTT GGGCCAGCCC TCAGATCCCT 
TCAAGAACAC AGTCTGTGAG GTCGCCTGTA CCTTATAAAA AACAGCTTAA TGAGGAGCTC 
CACCGGCGCT CTTCAGTGTT AGCAGAGAAC ACTTTGCCAC ATCCTCAGGA GAGCACAGAC 
TCCAGAAGGA AAGCAGAACC AGCCTGTGGA GGTCATGACC CACGTACAGC TGTTCAGCTT 
CGAAGCCTCA GCACAGTATT GAAACGCCTC AAAGAAATCA TGGAAGGAAA AAGCCAGGAC 
AGTGACCTGA AGCAATATTG GATGCCAGAT AGCCAGTGTA AAGAGTGCTA TGACTGCAGT 
GAAAAGTTTA CAACATTTAG GCGCAGACAC CATTGCAGAC TGTGTGGGCA GATTTTCTGC 
AGTCGTTGTT GTAATCAAGA AATCCCTGGA AAATTTATGG GCTATACAGG AGACCTCCGA 
GCATGCACCT ACTGTAGGAA AATAGCCTTA AGTTATGCTC ATTCCACAGA CAGTAATTCC 
ATTGGGGAAG ACTTGAATGC TCTTTCAGAT TCAACTTGCT CTGTGTCTAT ACTTGATCCA 
AGCGAACCTC GGACACCTGT TGGGAGTAGA AAAGCCAGTC GTAACATATT CTTAGAGGAT 
GATTTAGCCT GGCAAAGTTT GATTCATCCG GATTCCTCAA ATAGTGCTCT TTCAACAAGA 
CTCGTATCTG TTCAAGAGGA TGCTGGGAAG TCTCCTGCTC GGAACAGATC AGCCAGCATT 
ACTAATCTGT CACTGGATCG GTCTGGTTCT CCTATGGTTC CTTCATATGA GACATCTGTC 
AGTCCCCAGG CTAACCGAAA CTACATTAGG ACCGAGACGA CTGAGGATGA ACGCAAAATT 
CTTCTGGACA GTGCTCAGTT AAAGGATCTG TGGAAGAAAA TCTGCCATCA CACCAGTGGG 
ATGGAATTTC AAGATCACCG TTACTGGTTG AGAACACATC CCAACTGCAT TGTAGGAAAG 
GAATTAGTCA ACTGGCTAAT CAGAAATGGA CACATCGCTA CAAGGGCACA AGCTATAGCA 
ATTGGACAAG CAATGGTTGA TGGACGTTGG TTGGATTGTG TTAGTCATCA TGACCAGCTT 
TTCAGGGACG AATATGCGTT GTATAGACCA CTTCAGAGTA CAGAATTTTC TGAGACACCT 
TCTCCAGACA GTGACTCCGT GAACTCTGTG GAAGGACACT CCGAGCCATC CTGGTTTAAA 
GACATAAAAT TTGATGACAG TGACACAGAA CAGATTGCTG AAGAAGGTGA CGATAATTTG 
GCTAATTCTG CCAGTCCTAG CAAGCGCACC TCAGTCAGCA GTTTCCAGTC CACAGTGGAC 
AGTGACTCAG CCGCTTCTAT CAGCCTGAAC GTGGAGCTGG ACAACGTGAA CTTCCATATC 
AAGAAGCCCT CCAAGTACCC ACATGTGCCC CCTCACCCTG CTGACCAAAA AGGTAGGAGG 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Pikfyve ( XM_021160793.2 ) cDNA ORF clone, Mus caroli(Ryukyu mouse) -> XP_021016452.1 Mus caroli phosphoinositide kinase, FYVE-type zinc finger containing (Pikfyve), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OMv23070
Clone ID Related Accession (Same CDS sequence)	XM_021160793.2 , XM_021160800.1 , XM_021160793.1 , XM_021160800.2
Accession Version	XM_021160793.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6294bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-06-09
Organism	Mus caroli(Ryukyu mouse)
Product	1-phosphatidylinositol 3-phosphate 5-kinase isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_034570.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process On Jun 10, 2019 this sequence version replaced XM_021160793.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus caroli Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCACAG ATGACAAGAG TTCCCCGACA CTGGACTCTG CTAATGATTT GCCTCGCTCT 
CCCGCCAGTC CTTCTCACCT CACTCACTTT AAACCCTTGA CTCCTGACCA GGATGAGCCC 
CCCTTCAAGT CAGCATATAG TTCTTTTGTA AACCTTTTTC GCTTTAACAA AGAGCGGGGA 
GAAGGGGGCC AAGGAGAGCA GCAGTCTCCG AGTTCAAGTT GGGCCAGCCC TCAGATCCCT 
TCAAGAACAC AGTCTGTGAG GTCGCCTGTA CCTTATAAAA AACAGCTTAA TGAGGAGCTC 
CACCGGCGCT CTTCAGTGTT AGACTCCAGA AGGAAAGCAG AACCAGCCTG TGGAGGTCAT 
GACCCACGTA CAGCTGTTCA GCTTCGAAGC CTCAGCACAG TATTGAAACG CCTCAAAGAA 
ATCATGGAAG GAAAAAGCCA GGACAGTGAC CTGAAGCAAT ATTGGATGCC AGATAGCCAG 
TGTAAAGAGT GCTATGACTG CAGTGAAAAG TTTACAACAT TTAGGCGCAG ACACCATTGC 
AGACTGTGTG GGCAGATTTT CTGCAGTCGT TGTTGTAATC AAGAAATCCC TGGAAAATTT 
ATGGGCTATA CAGGAGACCT CCGAGCATGC ACCTACTGTA GGAAAATAGC CTTAAGTTAT 
GCTCATTCCA CAGACAGTAA TTCCATTGGG GAAGACTTGA ATGCTCTTTC AGATTCAACT 
TGCTCTGTGT CTATACTTGA TCCAAGCGAA CCTCGGACAC CTGTTGGGAG TAGAAAAGCC 
AGTCGTAACA TATTCTTAGA GGATGATTTA GCCTGGCAAA GTTTGATTCA TCCGGATTCC 
TCAAATAGTG CTCTTTCAAC AAGACTCGTA TCTGTTCAAG AGGATGCTGG GAAGTCTCCT 
GCTCGGAACA GATCAGCCAG CATTACTAAT CTGTCACTGG ATCGGTCTGG TTCTCCTATG 
GTTCCTTCAT ATGAGACATC TGTCAGTCCC CAGGCTAACC GAAACTACAT TAGGACCGAG 
ACGACTGAGG ATGAACGCAA AATTCTTCTG GACAGTGCTC AGTTAAAGGA TCTGTGGAAG 
AAAATCTGCC ATCACACCAG TGGGATGGAA TTTCAAGATC ACCGTTACTG GTTGAGAACA 
CATCCCAACT GCATTGTAGG AAAGGAATTA GTCAACTGGC TAATCAGAAA TGGACACATC 
GCTACAAGGG CACAAGCTAT AGCAATTGGA CAAGCAATGG TTGATGGACG TTGGTTGGAT 
TGTGTTAGTC ATCATGACCA GCTTTTCAGG GACGAATATG CGTTGTATAG ACCACTTCAG 
AGTACAGAAT TTTCTGAGAC ACCTTCTCCA GACAGTGACT CCGTGAACTC TGTGGAAGGA 
CACTCCGAGC CATCCTGGTT TAAAGACATA AAATTTGATG ACAGTGACAC AGAACAGATT 
GCTGAAGAAG GTGACGATAA TTTGGCTAAT TCTGCCAGTC CTAGCAAGCG CACCTCAGTC 
AGCAGTTTCC AGTCCACAGT GGACAGTGAC TCAGCCGCTT CTATCAGCCT GAACGTGGAG 
CTGGACAACG TGAACTTCCA TATCAAGAAG CCCTCCAAGT ACCCACATGT GCCCCCTCAC 
CCTGCTGACC AAAAAGAGTA TTTGGTTTCT GACACTGGAG GACAGCAGCT CTCAATAAGT 
GATGCCTTCA TCAAAGAGTC CTTATTTAAT CGACGAGTAG AGGAAAAATC CAAAGAGCTG 
CCTTTTACCC CTTTGGGCTG GCATCATAAC AACCTGGAAC TCTTGCGAGA GGAGAATGAG 
GAGAAGCAAG CCATGGAAAG GCTGCTTTCA GCTAATCATA ACCACATGAT GGCCCTACTC 
CAGCAATTGC TTCAAAACGA GTCATTGTCA TCGTCTTGGA GGGACATCAT TGTGTCACTA 
GTCTGCCAGG TTGTTCAGAC AGTCCGACCT GACGTCAAGC ACCAGGATGA TGACATGGAT 
ATCCGTCAGT TTGTCCATAT CAAGAAGATC CCAGGTGGAA AGAAATTTGA TTCTGTGGTT 
GTCAATGGCT TTGTTTGTAC CAAGAACATT GCACATAAAA AGATGAATTC CTGTATTAAA 
AACCCCAAAA TCCTTCTGTT GAAGTGTTCT ATTGAGTATC TCTATAGAGA AGAAACTAAG 
TTTACCTGCA TTGATCCTAT TGTGCTTCAG GAAAGGGAAT TCTTGAAGAA TTATGTTCAA 
CGAATAGTTG ATGTTCGACC CACATTGGTT CTTGTTGAGA AAACCGTGTC TCGGATTGCT 
CAGGACATGT TACTGGAACA TGGCATTACT CTGGTCATTA ATGTAAAGTC ACAAGTTTTA 
GAAAGAATCA GTCGAATGAC CCAAGGTGAT TTAGTGGTGT CCATGGACCA GCTGCTCACC 
AAACCCCACT TGGGCACTTG CCACAAATTT TATATGCAGA TATTTCAGCT GCCTAATGAA 
CAAACCAAAA CACTGATGTT TTTTGAAGGC TGTCCACAGC ACCTAGGCTG CACAATCAAG 
CTCAGAGGAG GCTCTGATTA TGAGCTGGCT CGAGTTAAGG AGATCCTAAT ATTTATGATC 
TGTGTAGCTT ATCATTCTCA GCTAGAAATC TCTTTTCTCA TGGATGAGTT CGCTATGCCT 
CCAACGTTAA TGCAAAGCCC TTCGTTCCAT CTTCTGACGG AGGGACGGGG TGAAGAGGGA 
GCCTCTCAGG AGCAGGTCAG TGGCAGCTCC CTTCCTCAGG ATCCTGAGTG CCCTCGTGAG 
GCCCTGTCTT CTGACGATAG CACTTTGTTG GAATCAAGGA CTGTGCTAGA GAAGGGTGAA 
CTAGACAATA AAAGTATTCC ACAAGCTGTT GCCTCTTTGA AGCATCAAGA TTATACCACC 
CCCACTTGCC CAGCAGGTAT CCCCTGTGCT CTCTTTGCAT TGGTACCAGA GTCATTGTTG 
CCTCTCCATA TGGATCAACA GGATGCTGTA GGAAATGAAC AACCAGAGAC ATCACAACAA 
ACAGATGAGC AACAGGATCC CAAAAGCCAG ATGAAAGCTT TTAGAGACCC TCTACAGGAT 
GACACCGGAA TGTATGTTAC TGAGGAAGTC ACCTCCTCTG AAGATCAACG AAAGACTTAT 
GCCTTGACAT TTAAACAGGA ATTAAAAGAC GTAATCCTCT GTATCTCTCC AGTTATTACA 
TTCCGTGAAC CTTTCCTTTT AACTGAAAAG GGGATGAGAT GCTCAACTCG AGATTATTTT 
CCAGAGCAGA TTTACTGGTC TCCTCTTCTC AACAAAGAGG TGATGGAAAT GGAGAGCAGG 
AGGAAGAAAC AGCTGCTCAG GGATCTCTCT GGACTTCAGG GCATGAATGG CAGTGTTCAG 
GCCAAGTCTA TTCAAGTCTT ACCCTCACAT GAACTAGTGA GCACCAGGAT TGCTGAACAT 
CTGGGTGACA GCCAGACCTT GGGTAGAATG CTAGCTGATT ATCGAGCTAG AGGAGGAAGA 
ATTCAGTCAA AACATTTGGA CCCTTTTGTC CATTCAAAAG ATGCATCATG TACTTCAGGT 
GGAAAACCAG GAAACAAAAC TGAGAGTGAT GAAGAGAGGG GATTGATCCC AAGTGATGTA 
ATATGGCCAG CAAAGGTGGA CTGTCTGAAT CCTGCTAACC ACCAGAGGCT CTGTGTGCTC 
TTCAGCAGCT CTTCTGCCCA GTCCAGCAAT GCTCCCAGTG CTTGTGTCAG TCCTTGGATT 
GTAACAATGG AGTTTTATGG AAAGAATGAC CTTACACTGG GAATATTTTT AGAAAGATAC 
TGTTTCAGGT CTTCTTACCA GTGTCCGAGC ATGTTCTGTG ACACCCCCAT GGTTCATCAC 
ATTCGACGCT TTGTTCATGG CCAAGGCTGT GTACAGATAA TTCTGAAGGA GTTGGATTCT 
CCAGTGCCTG GATATCAACA TACAATTCTC ACATACTCCT GGTGCAGAAT CTGCAAACAA 
GTAACACCAG TTGTTGCTCT TTCAAATGAA TCCTGGTCTA TGTCATTTGC AAAGTACCTT 
GAACTTCGAT TTTATGGCCA CCAGTACACA CGCAGAGCCA ACGCTGAGCC CTGCGGTCAC 
TCTATCCACC ATGATTATCA CCAGTATTTC TCTTATAACC AGATGGTGGC ATCTTTCAGT 
TACTCTCCTA TTCGGCTTCT TGAAGTATGT GTTCCACTAC CAAAAATATT CATTAAGCGT 
CAAGCCCCAC TGAAGGTATC TCTTCTTCAG GACCTCAAGG ACTTTTTTCA GAAGGTTTCA 
CAGGTGTACC TAGCTGTTGA TGAGAGACTT GCATCCTTGA AAACGGATAC ATTTAGCAAA 
ACCAGAGAGG AAAAGATGGA AGATATCTTT GCACAAAAGG AGATGGAAGA GGGTGAGTTT 
AAGAACTGGA CAGAGAAGAT GCAAGCAAGG CTCATGTCTT CCTCTGTGGA TACCCCTCAG 
CAACTGCAGT CCATTTTTGA GTCACTGATT GCCAAGAAGC AAAGCCTCTG TGAGGTGCTC 
CAGGCGTGGA ACAGCAGGTT GCAGGACCTC TTCCAGCAGG AAAAAGGTAG AAAGAGGCCT 
TCAGTTCCTC CCAGTCCTGG GAGACTGAGA CAAGGTGAAG AAAGCAAGAT AAATGCAATG 
GACACATCTC CAAGGAATAT TTCTCCAGGA CTTCACAATG GAGAAAAAGA GGATCGCTTC 
TTGACAACCC TGTCCAGCCA GAGCTCTACG AGCTCCACCC ACCTCCAGCT GCCCACTCCT 
CCCGAGGCCC TGGCCGAGCA GGTAGTGGGA GGGCCGACTG ATCTGGATTC AGCCAGTGGT 
TCTGAAGATG TATTTGATGG TCATTTGCTG GGATCCACAG ACAGCCAGGT GAAGGAAAAG 
TCAACCATGA AAGCCATCTT TGCTAATTTG CTTCCAGGAA ACAGCTACAA TCCCATTCCA 
TTTCCTTTTG ATCCAGATAA ACACTACTTA ATGTATGAAC ATGAACGGGT GCCCATTGCT 
GTCTGTGAGA AAGAGCCCAG CTCCATCATT GCTTTTGCAC TCAGTTGTAA AGAATACCGA 
AATGCCTTAG AGGAATTGTC CAAAGCGACT CTGTGGAACA GTGCTGAAGA AGGGCTTCCA 
GCCAATAGTG CTTTAGATAA CAGACCTAAG AGTAGCAGCC CTATTAGAGT ACCTGAAATC 
AGTGGAGGAC AGACAAACCG CACAGGAGAA GCAGAACCTC AGCCAACCAA AAAAGCTTCA 
GGCATGTTGT CCTTCTTCAG AGGAACAGCA GGGAAGAGTC CTGACCTGTC TTCTCAGAAG 
AGGGAGACCT TACGTGGGGC AGACAGTGCT TACTACCAGG TTGGGCAGGC TGGCAAGGAG 
GGGTTGGAGA GTCAAGGCCT GGAGCCTCAA GATGAAGTAG ACGGAGGAGA TACACAGAAG 
AAACAACTCA CAAATCCTCA TGTGGAACTT CAATTTTCTG ATGCTAATGC CAAGTTTTAC 
TGTCGGCTGT ACTACGCGGG AGAGTTCCAT AAGATGCGTG AAGTGATTCT GGGCAGCAGC 
GAGGAGGATT TCATTCGCTC CCTTTCTCAC TCATCTCCCT GGCAGGCCCG GGGAGGCAAG 
TCAGGAGCTG CTTTCTATGC CACCGAAGAT GATAGATTCA TTCTGAAGCA AATGCCTCGT 
TTGGAAGTCC AGTCTTTCCT TGACTTTGCA CCACACTACT TCAATTATAT CACAAATGCT 
GTTCAACAAA AGAGGCCCAC CGCCTTGGCT AAAATTCTTG GAGTTTACAG AATTGGCTAT 
AAGAACTCTC AGAACAACAC TGAGAAGAAG TTAGATCTCC TTGTTATGGA AAATCTTTTC 
TATGGGAGAA AGATGGCACA GGTTTTTGAT TTGAAGGGTT CACTTAGGAA TCGAAATGTA 
AAAACTGACA CTGGGAAAGA GAGCTGTGAT GTGGTTCTGT TGGATGAAAA CCTCCTAAAG 
ATGGTTCGAG ACAACCCTCT CTATATTCGT TCCCATTCCA AATCTGTGCT GAGAACCTCC 
ATCCACAGCG ACGCCCATTT CCTTTCCAGC CACCTCATTA TAGACTATTC TCTGCTGGTT 
GGGCGAGATG ACACTAGCAA TGAGCTTGTG GTTGGCATCA TAGATTACAT TCGAACATTT 
ACATGGGACA AAAAACTTGA GATGGTTGTG AAGTCAACAG GAATTTTAGG AGGACAAGGT 
AAAATGCCAA CTGTGGTCTC TCCAGAGTTG TATAGGACTA GATTTTGTGA AGCAATGGAC 
AAGTATTTCT TGATGGTGCC AGACCACTGG ACAGGGTTGG ATCTGAATTG CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021016452.1
CDS	164..6457
Translation	MATDDKSSPTLDSANDLPRSPASPSHLTHFKPLTPDQDEPPFKSAYSSFVNLFRFNKERGEGGQGEQQSPSSSWASPQIPSRTQSVRSPVPYKKQLNEELHRRSSVLDSRRKAEPACGGHDPRTAVQLRSLSTVLKRLKEIMEGKSQDSDLKQYWMPDSQCKECYDCSEKFTTFRRRHHCRLCGQIFCSRCCNQEIPGKFMGYTGDLRACTYCRKIALSYAHSTDSNSIGEDLNALSDSTCSVSILDPSEPRTPVGSRKASRNIFLEDDLAWQSLIHPDSSNSALSTRLVSVQEDAGKSPARNRSASITNLSLDRSGSPMVPSYETSVSPQANRNYIRTETTEDERKILLDSAQLKDLWKKICHHTSGMEFQDHRYWLRTHPNCIVGKELVNWLIRNGHIATRAQAIAIGQAMVDGRWLDCVSHHDQLFRDEYALYRPLQSTEFSETPSPDSDSVNSVEGHSEPSWFKDIKFDDSDTEQIAEEGDDNLANSASPSKRTSVSSFQSTVDSDSAASISLNVELDNVNFHIKKPSKYPHVPPHPADQKEYLVSDTGGQQLSISDAFIKESLFNRRVEEKSKELPFTPLGWHHNNLELLREENEEKQAMERLLSANHNHMMALLQQLLQNESLSSSWRDIIVSLVCQVVQTVRPDVKHQDDDMDIRQFVHIKKIPGGKKFDSVVVNGFVCTKNIAHKKMNSCIKNPKILLLKCSIEYLYREETKFTCIDPIVLQEREFLKNYVQRIVDVRPTLVLVEKTVSRIAQDMLLEHGITLVINVKSQVLERISRMTQGDLVVSMDQLLTKPHLGTCHKFYMQIFQLPNEQTKTLMFFEGCPQHLGCTIKLRGGSDYELARVKEILIFMICVAYHSQLEISFLMDEFAMPPTLMQSPSFHLLTEGRGEEGASQEQVSGSSLPQDPECPREALSSDDSTLLESRTVLEKGELDNKSIPQAVASLKHQDYTTPTCPAGIPCALFALVPESLLPLHMDQQDAVGNEQPETSQQTDEQQDPKSQMKAFRDPLQDDTGMYVTEEVTSSEDQRKTYALTFKQELKDVILCISPVITFREPFLLTEKGMRCSTRDYFPEQIYWSPLLNKEVMEMESRRKKQLLRDLSGLQGMNGSVQAKSIQVLPSHELVSTRIAEHLGDSQTLGRMLADYRARGGRIQSKHLDPFVHSKDASCTSGGKPGNKTESDEERGLIPSDVIWPAKVDCLNPANHQRLCVLFSSSSAQSSNAPSACVSPWIVTMEFYGKNDLTLGIFLERYCFRSSYQCPSMFCDTPMVHHIRRFVHGQGCVQIILKELDSPVPGYQHTILTYSWCRICKQVTPVVALSNESWSMSFAKYLELRFYGHQYTRRANAEPCGHSIHHDYHQYFSYNQMVASFSYSPIRLLEVCVPLPKIFIKRQAPLKVSLLQDLKDFFQKVSQVYLAVDERLASLKTDTFSKTREEKMEDIFAQKEMEEGEFKNWTEKMQARLMSSSVDTPQQLQSIFESLIAKKQSLCEVLQAWNSRLQDLFQQEKGRKRPSVPPSPGRLRQGEESKINAMDTSPRNISPGLHNGEKEDRFLTTLSSQSSTSSTHLQLPTPPEALAEQVVGGPTDLDSASGSEDVFDGHLLGSTDSQVKEKSTMKAIFANLLPGNSYNPIPFPFDPDKHYLMYEHERVPIAVCEKEPSSIIAFALSCKEYRNALEELSKATLWNSAEEGLPANSALDNRPKSSSPIRVPEISGGQTNRTGEAEPQPTKKASGMLSFFRGTAGKSPDLSSQKRETLRGADSAYYQVGQAGKEGLESQGLEPQDEVDGGDTQKKQLTNPHVELQFSDANAKFYCRLYYAGEFHKMREVILGSSEEDFIRSLSHSSPWQARGGKSGAAFYATEDDRFILKQMPRLEVQSFLDFAPHYFNYITNAVQQKRPTALAKILGVYRIGYKNSQNNTEKKLDLLVMENLFYGRKMAQVFDLKGSLRNRNVKTDTGKESCDVVLLDENLLKMVRDNPLYIRSHSKSVLRTSIHSDAHFLSSHLIIDYSLLVGRDDTSNELVVGIIDYIRTFTWDKKLEMVVKSTGILGGQGKMPTVVSPELYRTRFCEAMDKYFLMVPDHWTGLDLNC

Target ORF information:

RefSeq Version	XM_021160793.2
Organism	Mus caroli(Ryukyu mouse)
Definition	Mus caroli phosphoinositide kinase, FYVE-type zinc finger containing (Pikfyve), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021160793.2

ORF Insert Sequence:

ATGGCCACAG ATGACAAGAG TTCCCCGACA CTGGACTCTG CTAATGATTT GCCTCGCTCT 
CCCGCCAGTC CTTCTCACCT CACTCACTTT AAACCCTTGA CTCCTGACCA GGATGAGCCC 
CCCTTCAAGT CAGCATATAG TTCTTTTGTA AACCTTTTTC GCTTTAACAA AGAGCGGGGA 
GAAGGGGGCC AAGGAGAGCA GCAGTCTCCG AGTTCAAGTT GGGCCAGCCC TCAGATCCCT 
TCAAGAACAC AGTCTGTGAG GTCGCCTGTA CCTTATAAAA AACAGCTTAA TGAGGAGCTC 
CACCGGCGCT CTTCAGTGTT AGACTCCAGA AGGAAAGCAG AACCAGCCTG TGGAGGTCAT 
GACCCACGTA CAGCTGTTCA GCTTCGAAGC CTCAGCACAG TATTGAAACG CCTCAAAGAA 
ATCATGGAAG GAAAAAGCCA GGACAGTGAC CTGAAGCAAT ATTGGATGCC AGATAGCCAG 
TGTAAAGAGT GCTATGACTG CAGTGAAAAG TTTACAACAT TTAGGCGCAG ACACCATTGC 
AGACTGTGTG GGCAGATTTT CTGCAGTCGT TGTTGTAATC AAGAAATCCC TGGAAAATTT 
ATGGGCTATA CAGGAGACCT CCGAGCATGC ACCTACTGTA GGAAAATAGC CTTAAGTTAT 
GCTCATTCCA CAGACAGTAA TTCCATTGGG GAAGACTTGA ATGCTCTTTC AGATTCAACT 
TGCTCTGTGT CTATACTTGA TCCAAGCGAA CCTCGGACAC CTGTTGGGAG TAGAAAAGCC 
AGTCGTAACA TATTCTTAGA GGATGATTTA GCCTGGCAAA GTTTGATTCA TCCGGATTCC 
TCAAATAGTG CTCTTTCAAC AAGACTCGTA TCTGTTCAAG AGGATGCTGG GAAGTCTCCT 
GCTCGGAACA GATCAGCCAG CATTACTAAT CTGTCACTGG ATCGGTCTGG TTCTCCTATG 
GTTCCTTCAT ATGAGACATC TGTCAGTCCC CAGGCTAACC GAAACTACAT TAGGACCGAG 
ACGACTGAGG ATGAACGCAA AATTCTTCTG GACAGTGCTC AGTTAAAGGA TCTGTGGAAG 
AAAATCTGCC ATCACACCAG TGGGATGGAA TTTCAAGATC ACCGTTACTG GTTGAGAACA 
CATCCCAACT GCATTGTAGG AAAGGAATTA GTCAACTGGC TAATCAGAAA TGGACACATC 
GCTACAAGGG CACAAGCTAT AGCAATTGGA CAAGCAATGG TTGATGGACG TTGGTTGGAT 
TGTGTTAGTC ATCATGACCA GCTTTTCAGG GACGAATATG CGTTGTATAG ACCACTTCAG 
AGTACAGAAT TTTCTGAGAC ACCTTCTCCA GACAGTGACT CCGTGAACTC TGTGGAAGGA 
CACTCCGAGC CATCCTGGTT TAAAGACATA AAATTTGATG ACAGTGACAC AGAACAGATT 
GCTGAAGAAG GTGACGATAA TTTGGCTAAT TCTGCCAGTC CTAGCAAGCG CACCTCAGTC 
AGCAGTTTCC AGTCCACAGT GGACAGTGAC TCAGCCGCTT CTATCAGCCT GAACGTGGAG 
CTGGACAACG TGAACTTCCA TATCAAGAAG CCCTCCAAGT ACCCACATGT GCCCCCTCAC 
CCTGCTGACC AAAAAGAGTA TTTGGTTTCT GACACTGGAG GACAGCAGCT CTCAATAAGT 
GATGCCTTCA TCAAAGAGTC CTTATTTAAT CGACGAGTAG AGGAAAAATC CAAAGAGCTG 
CCTTTTACCC CTTTGGGCTG GCATCATAAC AACCTGGAAC TCTTGCGAGA GGAGAATGAG 
GAGAAGCAAG CCATGGAAAG GCTGCTTTCA GCTAATCATA ACCACATGAT GGCCCTACTC 
CAGCAATTGC TTCAAAACGA GTCATTGTCA TCGTCTTGGA GGGACATCAT TGTGTCACTA 
GTCTGCCAGG TTGTTCAGAC AGTCCGACCT GACGTCAAGC ACCAGGATGA TGACATGGAT 
ATCCGTCAGT TTGTCCATAT CAAGAAGATC CCAGGTGGAA AGAAATTTGA TTCTGTGGTT 
GTCAATGGCT TTGTTTGTAC CAAGAACATT GCACATAAAA AGATGAATTC CTGTATTAAA 
AACCCCAAAA TCCTTCTGTT GAAGTGTTCT ATTGAGTATC TCTATAGAGA AGAAACTAAG 
TTTACCTGCA TTGATCCTAT TGTGCTTCAG GAAAGGGAAT TCTTGAAGAA TTATGTTCAA 
CGAATAGTTG ATGTTCGACC CACATTGGTT CTTGTTGAGA AAACCGTGTC TCGGATTGCT 
CAGGACATGT TACTGGAACA TGGCATTACT CTGGTCATTA ATGTAAAGTC ACAAGTTTTA 
GAAAGAATCA GTCGAATGAC CCAAGGTGAT TTAGTGGTGT CCATGGACCA GCTGCTCACC 
AAACCCCACT TGGGCACTTG CCACAAATTT TATATGCAGA TATTTCAGCT GCCTAATGAA 
CAAACCAAAA CACTGATGTT TTTTGAAGGC TGTCCACAGC ACCTAGGCTG CACAATCAAG 
CTCAGAGGAG GCTCTGATTA TGAGCTGGCT CGAGTTAAGG AGATCCTAAT ATTTATGATC 
TGTGTAGCTT ATCATTCTCA GCTAGAAATC TCTTTTCTCA TGGATGAGTT CGCTATGCCT 
CCAACGTTAA TGCAAAGCCC TTCGTTCCAT CTTCTGACGG AGGGACGGGG TGAAGAGGGA 
GCCTCTCAGG AGCAGGTCAG TGGCAGCTCC CTTCCTCAGG ATCCTGAGTG CCCTCGTGAG 
GCCCTGTCTT CTGACGATAG CACTTTGTTG GAATCAAGGA CTGTGCTAGA GAAGGGTGAA 
CTAGACAATA AAAGTATTCC ACAAGCTGTT GCCTCTTTGA AGCATCAAGA TTATACCACC 
CCCACTTGCC CAGCAGGTAT CCCCTGTGCT CTCTTTGCAT TGGTACCAGA GTCATTGTTG 
CCTCTCCATA TGGATCAACA GGATGCTGTA GGAAATGAAC AACCAGAGAC ATCACAACAA 
ACAGATGAGC AACAGGATCC CAAAAGCCAG ATGAAAGCTT TTAGAGACCC TCTACAGGAT 
GACACCGGAA TGTATGTTAC TGAGGAAGTC ACCTCCTCTG AAGATCAACG AAAGACTTAT 
GCCTTGACAT TTAAACAGGA ATTAAAAGAC GTAATCCTCT GTATCTCTCC AGTTATTACA 
TTCCGTGAAC CTTTCCTTTT AACTGAAAAG GGGATGAGAT GCTCAACTCG AGATTATTTT 
CCAGAGCAGA TTTACTGGTC TCCTCTTCTC AACAAAGAGG TGATGGAAAT GGAGAGCAGG 
AGGAAGAAAC AGCTGCTCAG GGATCTCTCT GGACTTCAGG GCATGAATGG CAGTGTTCAG 
GCCAAGTCTA TTCAAGTCTT ACCCTCACAT GAACTAGTGA GCACCAGGAT TGCTGAACAT 
CTGGGTGACA GCCAGACCTT GGGTAGAATG CTAGCTGATT ATCGAGCTAG AGGAGGAAGA 
ATTCAGTCAA AACATTTGGA CCCTTTTGTC CATTCAAAAG ATGCATCATG TACTTCAGGT 
GGAAAACCAG GAAACAAAAC TGAGAGTGAT GAAGAGAGGG GATTGATCCC AAGTGATGTA 
ATATGGCCAG CAAAGGTGGA CTGTCTGAAT CCTGCTAACC ACCAGAGGCT CTGTGTGCTC 
TTCAGCAGCT CTTCTGCCCA GTCCAGCAAT GCTCCCAGTG CTTGTGTCAG TCCTTGGATT 
GTAACAATGG AGTTTTATGG AAAGAATGAC CTTACACTGG GAATATTTTT AGAAAGATAC 
TGTTTCAGGT CTTCTTACCA GTGTCCGAGC ATGTTCTGTG ACACCCCCAT GGTTCATCAC 
ATTCGACGCT TTGTTCATGG CCAAGGCTGT GTACAGATAA TTCTGAAGGA GTTGGATTCT 
CCAGTGCCTG GATATCAACA TACAATTCTC ACATACTCCT GGTGCAGAAT CTGCAAACAA 
GTAACACCAG TTGTTGCTCT TTCAAATGAA TCCTGGTCTA TGTCATTTGC AAAGTACCTT 
GAACTTCGAT TTTATGGCCA CCAGTACACA CGCAGAGCCA ACGCTGAGCC CTGCGGTCAC 
TCTATCCACC ATGATTATCA CCAGTATTTC TCTTATAACC AGATGGTGGC ATCTTTCAGT 
TACTCTCCTA TTCGGCTTCT TGAAGTATGT GTTCCACTAC CAAAAATATT CATTAAGCGT 
CAAGCCCCAC TGAAGGTATC TCTTCTTCAG GACCTCAAGG ACTTTTTTCA GAAGGTTTCA 
CAGGTGTACC TAGCTGTTGA TGAGAGACTT GCATCCTTGA AAACGGATAC ATTTAGCAAA 
ACCAGAGAGG AAAAGATGGA AGATATCTTT GCACAAAAGG AGATGGAAGA GGGTGAGTTT 
AAGAACTGGA CAGAGAAGAT GCAAGCAAGG CTCATGTCTT CCTCTGTGGA TACCCCTCAG 
CAACTGCAGT CCATTTTTGA GTCACTGATT GCCAAGAAGC AAAGCCTCTG TGAGGTGCTC 
CAGGCGTGGA ACAGCAGGTT GCAGGACCTC TTCCAGCAGG AAAAAGGTAG AAAGAGGCCT 
TCAGTTCCTC CCAGTCCTGG GAGACTGAGA CAAGGTGAAG AAAGCAAGAT AAATGCAATG 
GACACATCTC CAAGGAATAT TTCTCCAGGA CTTCACAATG GAGAAAAAGA GGATCGCTTC 
TTGACAACCC TGTCCAGCCA GAGCTCTACG AGCTCCACCC ACCTCCAGCT GCCCACTCCT 
CCCGAGGCCC TGGCCGAGCA GGTAGTGGGA GGGCCGACTG ATCTGGATTC AGCCAGTGGT 
TCTGAAGATG TATTTGATGG TCATTTGCTG GGATCCACAG ACAGCCAGGT GAAGGAAAAG 
TCAACCATGA AAGCCATCTT TGCTAATTTG CTTCCAGGAA ACAGCTACAA TCCCATTCCA 
TTTCCTTTTG ATCCAGATAA ACACTACTTA ATGTATGAAC ATGAACGGGT GCCCATTGCT 
GTCTGTGAGA AAGAGCCCAG CTCCATCATT GCTTTTGCAC TCAGTTGTAA AGAATACCGA 
AATGCCTTAG AGGAATTGTC CAAAGCGACT CTGTGGAACA GTGCTGAAGA AGGGCTTCCA 
GCCAATAGTG CTTTAGATAA CAGACCTAAG AGTAGCAGCC CTATTAGAGT ACCTGAAATC 
AGTGGAGGAC AGACAAACCG CACAGGAGAA GCAGAACCTC AGCCAACCAA AAAAGCTTCA 
GGCATGTTGT CCTTCTTCAG AGGAACAGCA GGGAAGAGTC CTGACCTGTC TTCTCAGAAG 
AGGGAGACCT TACGTGGGGC AGACAGTGCT TACTACCAGG TTGGGCAGGC TGGCAAGGAG 
GGGTTGGAGA GTCAAGGCCT GGAGCCTCAA GATGAAGTAG ACGGAGGAGA TACACAGAAG 
AAACAACTCA CAAATCCTCA TGTGGAACTT CAATTTTCTG ATGCTAATGC CAAGTTTTAC 
TGTCGGCTGT ACTACGCGGG AGAGTTCCAT AAGATGCGTG AAGTGATTCT GGGCAGCAGC 
GAGGAGGATT TCATTCGCTC CCTTTCTCAC TCATCTCCCT GGCAGGCCCG GGGAGGCAAG 
TCAGGAGCTG CTTTCTATGC CACCGAAGAT GATAGATTCA TTCTGAAGCA AATGCCTCGT 
TTGGAAGTCC AGTCTTTCCT TGACTTTGCA CCACACTACT TCAATTATAT CACAAATGCT 
GTTCAACAAA AGAGGCCCAC CGCCTTGGCT AAAATTCTTG GAGTTTACAG AATTGGCTAT 
AAGAACTCTC AGAACAACAC TGAGAAGAAG TTAGATCTCC TTGTTATGGA AAATCTTTTC 
TATGGGAGAA AGATGGCACA GGTTTTTGAT TTGAAGGGTT CACTTAGGAA TCGAAATGTA 
AAAACTGACA CTGGGAAAGA GAGCTGTGAT GTGGTTCTGT TGGATGAAAA CCTCCTAAAG 
ATGGTTCGAG ACAACCCTCT CTATATTCGT TCCCATTCCA AATCTGTGCT GAGAACCTCC 
ATCCACAGCG ACGCCCATTT CCTTTCCAGC CACCTCATTA TAGACTATTC TCTGCTGGTT 
GGGCGAGATG ACACTAGCAA TGAGCTTGTG GTTGGCATCA TAGATTACAT TCGAACATTT 
ACATGGGACA AAAAACTTGA GATGGTTGTG AAGTCAACAG GAATTTTAGG AGGACAAGGT 
AAAATGCCAA CTGTGGTCTC TCCAGAGTTG TATAGGACTA GATTTTGTGA AGCAATGGAC 
AAGTATTTCT TGATGGTGCC AGACCACTGG ACAGGGTTGG ATCTGAATTG CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Pikfyve ( XM_021160800.1 ) cDNA ORF clone, Mus caroli(Ryukyu mouse) -> XP_021016459.1 Mus caroli phosphoinositide kinase, FYVE-type zinc finger containing (Pikfyve), transcript variant X2, mRNA.

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CloneID	OMv23070
Clone ID Related Accession (Same CDS sequence)	XM_021160793.2 , XM_021160800.1 , XM_021160793.1 , XM_021160800.2
Accession Version	XM_021160800.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6294bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-05-20
Organism	Mus caroli(Ryukyu mouse)
Product	1-phosphatidylinositol 3-phosphate 5-kinase
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_034570.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus caroli Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCACAG ATGACAAGAG TTCCCCGACA CTGGACTCTG CTAATGATTT GCCTCGCTCT 
CCCGCCAGTC CTTCTCACCT CACTCACTTT AAACCCTTGA CTCCTGACCA GGATGAGCCC 
CCCTTCAAGT CAGCATATAG TTCTTTTGTA AACCTTTTTC GCTTTAACAA AGAGCGGGGA 
GAAGGGGGCC AAGGAGAGCA GCAGTCTCCG AGTTCAAGTT GGGCCAGCCC TCAGATCCCT 
TCAAGAACAC AGTCTGTGAG GTCGCCTGTA CCTTATAAAA AACAGCTTAA TGAGGAGCTC 
CACCGGCGCT CTTCAGTGTT AGACTCCAGA AGGAAAGCAG AACCAGCCTG TGGAGGTCAT 
GACCCACGTA CAGCTGTTCA GCTTCGAAGC CTCAGCACAG TATTGAAACG CCTCAAAGAA 
ATCATGGAAG GAAAAAGCCA GGACAGTGAC CTGAAGCAAT ATTGGATGCC AGATAGCCAG 
TGTAAAGAGT GCTATGACTG CAGTGAAAAG TTTACAACAT TTAGGCGCAG ACACCATTGC 
AGACTGTGTG GGCAGATTTT CTGCAGTCGT TGTTGTAATC AAGAAATCCC TGGAAAATTT 
ATGGGCTATA CAGGAGACCT CCGAGCATGC ACCTACTGTA GGAAAATAGC CTTAAGTTAT 
GCTCATTCCA CAGACAGTAA TTCCATTGGG GAAGACTTGA ATGCTCTTTC AGATTCAACT 
TGCTCTGTGT CTATACTTGA TCCAAGCGAA CCTCGGACAC CTGTTGGGAG TAGAAAAGCC 
AGTCGTAACA TATTCTTAGA GGATGATTTA GCCTGGCAAA GTTTGATTCA TCCGGATTCC 
TCAAATAGTG CTCTTTCAAC AAGACTCGTA TCTGTTCAAG AGGATGCTGG GAAGTCTCCT 
GCTCGGAACA GATCAGCCAG CATTACTAAT CTGTCACTGG ATCGGTCTGG TTCTCCTATG 
GTTCCTTCAT ATGAGACATC TGTCAGTCCC CAGGCTAACC GAAACTACAT TAGGACCGAG 
ACGACTGAGG ATGAACGCAA AATTCTTCTG GACAGTGCTC AGTTAAAGGA TCTGTGGAAG 
AAAATCTGCC ATCACACCAG TGGGATGGAA TTTCAAGATC ACCGTTACTG GTTGAGAACA 
CATCCCAACT GCATTGTAGG AAAGGAATTA GTCAACTGGC TAATCAGAAA TGGACACATC 
GCTACAAGGG CACAAGCTAT AGCAATTGGA CAAGCAATGG TTGATGGACG TTGGTTGGAT 
TGTGTTAGTC ATCATGACCA GCTTTTCAGG GACGAATATG CGTTGTATAG ACCACTTCAG 
AGTACAGAAT TTTCTGAGAC ACCTTCTCCA GACAGTGACT CCGTGAACTC TGTGGAAGGA 
CACTCCGAGC CATCCTGGTT TAAAGACATA AAATTTGATG ACAGTGACAC AGAACAGATT 
GCTGAAGAAG GTGACGATAA TTTGGCTAAT TCTGCCAGTC CTAGCAAGCG CACCTCAGTC 
AGCAGTTTCC AGTCCACAGT GGACAGTGAC TCAGCCGCTT CTATCAGCCT GAACGTGGAG 
CTGGACAACG TGAACTTCCA TATCAAGAAG CCCTCCAAGT ACCCACATGT GCCCCCTCAC 
CCTGCTGACC AAAAAGAGTA TTTGGTTTCT GACACTGGAG GACAGCAGCT CTCAATAAGT 
GATGCCTTCA TCAAAGAGTC CTTATTTAAT CGACGAGTAG AGGAAAAATC CAAAGAGCTG 
CCTTTTACCC CTTTGGGCTG GCATCATAAC AACCTGGAAC TCTTGCGAGA GGAGAATGAG 
GAGAAGCAAG CCATGGAAAG GCTGCTTTCA GCTAATCATA ACCACATGAT GGCCCTACTC 
CAGCAATTGC TTCAAAACGA GTCATTGTCA TCGTCTTGGA GGGACATCAT TGTGTCACTA 
GTCTGCCAGG TTGTTCAGAC AGTCCGACCT GACGTCAAGC ACCAGGATGA TGACATGGAT 
ATCCGTCAGT TTGTCCATAT CAAGAAGATC CCAGGTGGAA AGAAATTTGA TTCTGTGGTT 
GTCAATGGCT TTGTTTGTAC CAAGAACATT GCACATAAAA AGATGAATTC CTGTATTAAA 
AACCCCAAAA TCCTTCTGTT GAAGTGTTCT ATTGAGTATC TCTATAGAGA AGAAACTAAG 
TTTACCTGCA TTGATCCTAT TGTGCTTCAG GAAAGGGAAT TCTTGAAGAA TTATGTTCAA 
CGAATAGTTG ATGTTCGACC CACATTGGTT CTTGTTGAGA AAACCGTGTC TCGGATTGCT 
CAGGACATGT TACTGGAACA TGGCATTACT CTGGTCATTA ATGTAAAGTC ACAAGTTTTA 
GAAAGAATCA GTCGAATGAC CCAAGGTGAT TTAGTGGTGT CCATGGACCA GCTGCTCACC 
AAACCCCACT TGGGCACTTG CCACAAATTT TATATGCAGA TATTTCAGCT GCCTAATGAA 
CAAACCAAAA CACTGATGTT TTTTGAAGGC TGTCCACAGC ACCTAGGCTG CACAATCAAG 
CTCAGAGGAG GCTCTGATTA TGAGCTGGCT CGAGTTAAGG AGATCCTAAT ATTTATGATC 
TGTGTAGCTT ATCATTCTCA GCTAGAAATC TCTTTTCTCA TGGATGAGTT CGCTATGCCT 
CCAACGTTAA TGCAAAGCCC TTCGTTCCAT CTTCTGACGG AGGGACGGGG TGAAGAGGGA 
GCCTCTCAGG AGCAGGTCAG TGGCAGCTCC CTTCCTCAGG ATCCTGAGTG CCCTCGTGAG 
GCCCTGTCTT CTGACGATAG CACTTTGTTG GAATCAAGGA CTGTGCTAGA GAAGGGTGAA 
CTAGACAATA AAAGTATTCC ACAAGCTGTT GCCTCTTTGA AGCATCAAGA TTATACCACC 
CCCACTTGCC CAGCAGGTAT CCCCTGTGCT CTCTTTGCAT TGGTACCAGA GTCATTGTTG 
CCTCTCCATA TGGATCAACA GGATGCTGTA GGAAATGAAC AACCAGAGAC ATCACAACAA 
ACAGATGAGC AACAGGATCC CAAAAGCCAG ATGAAAGCTT TTAGAGACCC TCTACAGGAT 
GACACCGGAA TGTATGTTAC TGAGGAAGTC ACCTCCTCTG AAGATCAACG AAAGACTTAT 
GCCTTGACAT TTAAACAGGA ATTAAAAGAC GTAATCCTCT GTATCTCTCC AGTTATTACA 
TTCCGTGAAC CTTTCCTTTT AACTGAAAAG GGGATGAGAT GCTCAACTCG AGATTATTTT 
CCAGAGCAGA TTTACTGGTC TCCTCTTCTC AACAAAGAGG TGATGGAAAT GGAGAGCAGG 
AGGAAGAAAC AGCTGCTCAG GGATCTCTCT GGACTTCAGG GCATGAATGG CAGTGTTCAG 
GCCAAGTCTA TTCAAGTCTT ACCCTCACAT GAACTAGTGA GCACCAGGAT TGCTGAACAT 
CTGGGTGACA GCCAGACCTT GGGTAGAATG CTAGCTGATT ATCGAGCTAG AGGAGGAAGA 
ATTCAGTCAA AACATTTGGA CCCTTTTGTC CATTCAAAAG ATGCATCATG TACTTCAGGT 
GGAAAACCAG GAAACAAAAC TGAGAGTGAT GAAGAGAGGG GATTGATCCC AAGTGATGTA 
ATATGGCCAG CAAAGGTGGA CTGTCTGAAT CCTGCTAACC ACCAGAGGCT CTGTGTGCTC 
TTCAGCAGCT CTTCTGCCCA GTCCAGCAAT GCTCCCAGTG CTTGTGTCAG TCCTTGGATT 
GTAACAATGG AGTTTTATGG AAAGAATGAC CTTACACTGG GAATATTTTT AGAAAGATAC 
TGTTTCAGGT CTTCTTACCA GTGTCCGAGC ATGTTCTGTG ACACCCCCAT GGTTCATCAC 
ATTCGACGCT TTGTTCATGG CCAAGGCTGT GTACAGATAA TTCTGAAGGA GTTGGATTCT 
CCAGTGCCTG GATATCAACA TACAATTCTC ACATACTCCT GGTGCAGAAT CTGCAAACAA 
GTAACACCAG TTGTTGCTCT TTCAAATGAA TCCTGGTCTA TGTCATTTGC AAAGTACCTT 
GAACTTCGAT TTTATGGCCA CCAGTACACA CGCAGAGCCA ACGCTGAGCC CTGCGGTCAC 
TCTATCCACC ATGATTATCA CCAGTATTTC TCTTATAACC AGATGGTGGC ATCTTTCAGT 
TACTCTCCTA TTCGGCTTCT TGAAGTATGT GTTCCACTAC CAAAAATATT CATTAAGCGT 
CAAGCCCCAC TGAAGGTATC TCTTCTTCAG GACCTCAAGG ACTTTTTTCA GAAGGTTTCA 
CAGGTGTACC TAGCTGTTGA TGAGAGACTT GCATCCTTGA AAACGGATAC ATTTAGCAAA 
ACCAGAGAGG AAAAGATGGA AGATATCTTT GCACAAAAGG AGATGGAAGA GGGTGAGTTT 
AAGAACTGGA CAGAGAAGAT GCAAGCAAGG CTCATGTCTT CCTCTGTGGA TACCCCTCAG 
CAACTGCAGT CCATTTTTGA GTCACTGATT GCCAAGAAGC AAAGCCTCTG TGAGGTGCTC 
CAGGCGTGGA ACAGCAGGTT GCAGGACCTC TTCCAGCAGG AAAAAGGTAG AAAGAGGCCT 
TCAGTTCCTC CCAGTCCTGG GAGACTGAGA CAAGGTGAAG AAAGCAAGAT AAATGCAATG 
GACACATCTC CAAGGAATAT TTCTCCAGGA CTTCACAATG GAGAAAAAGA GGATCGCTTC 
TTGACAACCC TGTCCAGCCA GAGCTCTACG AGCTCCACCC ACCTCCAGCT GCCCACTCCT 
CCCGAGGCCC TGGCCGAGCA GGTAGTGGGA GGGCCGACTG ATCTGGATTC AGCCAGTGGT 
TCTGAAGATG TATTTGATGG TCATTTGCTG GGATCCACAG ACAGCCAGGT GAAGGAAAAG 
TCAACCATGA AAGCCATCTT TGCTAATTTG CTTCCAGGAA ACAGCTACAA TCCCATTCCA 
TTTCCTTTTG ATCCAGATAA ACACTACTTA ATGTATGAAC ATGAACGGGT GCCCATTGCT 
GTCTGTGAGA AAGAGCCCAG CTCCATCATT GCTTTTGCAC TCAGTTGTAA AGAATACCGA 
AATGCCTTAG AGGAATTGTC CAAAGCGACT CTGTGGAACA GTGCTGAAGA AGGGCTTCCA 
GCCAATAGTG CTTTAGATAA CAGACCTAAG AGTAGCAGCC CTATTAGAGT ACCTGAAATC 
AGTGGAGGAC AGACAAACCG CACAGGAGAA GCAGAACCTC AGCCAACCAA AAAAGCTTCA 
GGCATGTTGT CCTTCTTCAG AGGAACAGCA GGGAAGAGTC CTGACCTGTC TTCTCAGAAG 
AGGGAGACCT TACGTGGGGC AGACAGTGCT TACTACCAGG TTGGGCAGGC TGGCAAGGAG 
GGGTTGGAGA GTCAAGGCCT GGAGCCTCAA GATGAAGTAG ACGGAGGAGA TACACAGAAG 
AAACAACTCA CAAATCCTCA TGTGGAACTT CAATTTTCTG ATGCTAATGC CAAGTTTTAC 
TGTCGGCTGT ACTACGCGGG AGAGTTCCAT AAGATGCGTG AAGTGATTCT GGGCAGCAGC 
GAGGAGGATT TCATTCGCTC CCTTTCTCAC TCATCTCCCT GGCAGGCCCG GGGAGGCAAG 
TCAGGAGCTG CTTTCTATGC CACCGAAGAT GATAGATTCA TTCTGAAGCA AATGCCTCGT 
TTGGAAGTCC AGTCTTTCCT TGACTTTGCA CCACACTACT TCAATTATAT CACAAATGCT 
GTTCAACAAA AGAGGCCCAC CGCCTTGGCT AAAATTCTTG GAGTTTACAG AATTGGCTAT 
AAGAACTCTC AGAACAACAC TGAGAAGAAG TTAGATCTCC TTGTTATGGA AAATCTTTTC 
TATGGGAGAA AGATGGCACA GGTTTTTGAT TTGAAGGGTT CACTTAGGAA TCGAAATGTA 
AAAACTGACA CTGGGAAAGA GAGCTGTGAT GTGGTTCTGT TGGATGAAAA CCTCCTAAAG 
ATGGTTCGAG ACAACCCTCT CTATATTCGT TCCCATTCCA AATCTGTGCT GAGAACCTCC 
ATCCACAGCG ACGCCCATTT CCTTTCCAGC CACCTCATTA TAGACTATTC TCTGCTGGTT 
GGGCGAGATG ACACTAGCAA TGAGCTTGTG GTTGGCATCA TAGATTACAT TCGAACATTT 
ACATGGGACA AAAAACTTGA GATGGTTGTG AAGTCAACAG GAATTTTAGG AGGACAAGGT 
AAAATGCCAA CTGTGGTCTC TCCAGAGTTG TATAGGACTA GATTTTGTGA AGCAATGGAC 
AAGTATTTCT TGATGGTGCC AGACCACTGG ACAGGGTTGG ATCTGAATTG CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021016459.1
CDS	76..6369
Translation	MATDDKSSPTLDSANDLPRSPASPSHLTHFKPLTPDQDEPPFKSAYSSFVNLFRFNKERGEGGQGEQQSPSSSWASPQIPSRTQSVRSPVPYKKQLNEELHRRSSVLDSRRKAEPACGGHDPRTAVQLRSLSTVLKRLKEIMEGKSQDSDLKQYWMPDSQCKECYDCSEKFTTFRRRHHCRLCGQIFCSRCCNQEIPGKFMGYTGDLRACTYCRKIALSYAHSTDSNSIGEDLNALSDSTCSVSILDPSEPRTPVGSRKASRNIFLEDDLAWQSLIHPDSSNSALSTRLVSVQEDAGKSPARNRSASITNLSLDRSGSPMVPSYETSVSPQANRNYIRTETTEDERKILLDSAQLKDLWKKICHHTSGMEFQDHRYWLRTHPNCIVGKELVNWLIRNGHIATRAQAIAIGQAMVDGRWLDCVSHHDQLFRDEYALYRPLQSTEFSETPSPDSDSVNSVEGHSEPSWFKDIKFDDSDTEQIAEEGDDNLANSASPSKRTSVSSFQSTVDSDSAASISLNVELDNVNFHIKKPSKYPHVPPHPADQKEYLVSDTGGQQLSISDAFIKESLFNRRVEEKSKELPFTPLGWHHNNLELLREENEEKQAMERLLSANHNHMMALLQQLLQNESLSSSWRDIIVSLVCQVVQTVRPDVKHQDDDMDIRQFVHIKKIPGGKKFDSVVVNGFVCTKNIAHKKMNSCIKNPKILLLKCSIEYLYREETKFTCIDPIVLQEREFLKNYVQRIVDVRPTLVLVEKTVSRIAQDMLLEHGITLVINVKSQVLERISRMTQGDLVVSMDQLLTKPHLGTCHKFYMQIFQLPNEQTKTLMFFEGCPQHLGCTIKLRGGSDYELARVKEILIFMICVAYHSQLEISFLMDEFAMPPTLMQSPSFHLLTEGRGEEGASQEQVSGSSLPQDPECPREALSSDDSTLLESRTVLEKGELDNKSIPQAVASLKHQDYTTPTCPAGIPCALFALVPESLLPLHMDQQDAVGNEQPETSQQTDEQQDPKSQMKAFRDPLQDDTGMYVTEEVTSSEDQRKTYALTFKQELKDVILCISPVITFREPFLLTEKGMRCSTRDYFPEQIYWSPLLNKEVMEMESRRKKQLLRDLSGLQGMNGSVQAKSIQVLPSHELVSTRIAEHLGDSQTLGRMLADYRARGGRIQSKHLDPFVHSKDASCTSGGKPGNKTESDEERGLIPSDVIWPAKVDCLNPANHQRLCVLFSSSSAQSSNAPSACVSPWIVTMEFYGKNDLTLGIFLERYCFRSSYQCPSMFCDTPMVHHIRRFVHGQGCVQIILKELDSPVPGYQHTILTYSWCRICKQVTPVVALSNESWSMSFAKYLELRFYGHQYTRRANAEPCGHSIHHDYHQYFSYNQMVASFSYSPIRLLEVCVPLPKIFIKRQAPLKVSLLQDLKDFFQKVSQVYLAVDERLASLKTDTFSKTREEKMEDIFAQKEMEEGEFKNWTEKMQARLMSSSVDTPQQLQSIFESLIAKKQSLCEVLQAWNSRLQDLFQQEKGRKRPSVPPSPGRLRQGEESKINAMDTSPRNISPGLHNGEKEDRFLTTLSSQSSTSSTHLQLPTPPEALAEQVVGGPTDLDSASGSEDVFDGHLLGSTDSQVKEKSTMKAIFANLLPGNSYNPIPFPFDPDKHYLMYEHERVPIAVCEKEPSSIIAFALSCKEYRNALEELSKATLWNSAEEGLPANSALDNRPKSSSPIRVPEISGGQTNRTGEAEPQPTKKASGMLSFFRGTAGKSPDLSSQKRETLRGADSAYYQVGQAGKEGLESQGLEPQDEVDGGDTQKKQLTNPHVELQFSDANAKFYCRLYYAGEFHKMREVILGSSEEDFIRSLSHSSPWQARGGKSGAAFYATEDDRFILKQMPRLEVQSFLDFAPHYFNYITNAVQQKRPTALAKILGVYRIGYKNSQNNTEKKLDLLVMENLFYGRKMAQVFDLKGSLRNRNVKTDTGKESCDVVLLDENLLKMVRDNPLYIRSHSKSVLRTSIHSDAHFLSSHLIIDYSLLVGRDDTSNELVVGIIDYIRTFTWDKKLEMVVKSTGILGGQGKMPTVVSPELYRTRFCEAMDKYFLMVPDHWTGLDLNC

Target ORF information:

RefSeq Version	XM_021160800.1
Organism	Mus caroli(Ryukyu mouse)
Definition	Mus caroli phosphoinositide kinase, FYVE-type zinc finger containing (Pikfyve), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021160800.1

ORF Insert Sequence:

ATGGCCACAG ATGACAAGAG TTCCCCGACA CTGGACTCTG CTAATGATTT GCCTCGCTCT 
CCCGCCAGTC CTTCTCACCT CACTCACTTT AAACCCTTGA CTCCTGACCA GGATGAGCCC 
CCCTTCAAGT CAGCATATAG TTCTTTTGTA AACCTTTTTC GCTTTAACAA AGAGCGGGGA 
GAAGGGGGCC AAGGAGAGCA GCAGTCTCCG AGTTCAAGTT GGGCCAGCCC TCAGATCCCT 
TCAAGAACAC AGTCTGTGAG GTCGCCTGTA CCTTATAAAA AACAGCTTAA TGAGGAGCTC 
CACCGGCGCT CTTCAGTGTT AGACTCCAGA AGGAAAGCAG AACCAGCCTG TGGAGGTCAT 
GACCCACGTA CAGCTGTTCA GCTTCGAAGC CTCAGCACAG TATTGAAACG CCTCAAAGAA 
ATCATGGAAG GAAAAAGCCA GGACAGTGAC CTGAAGCAAT ATTGGATGCC AGATAGCCAG 
TGTAAAGAGT GCTATGACTG CAGTGAAAAG TTTACAACAT TTAGGCGCAG ACACCATTGC 
AGACTGTGTG GGCAGATTTT CTGCAGTCGT TGTTGTAATC AAGAAATCCC TGGAAAATTT 
ATGGGCTATA CAGGAGACCT CCGAGCATGC ACCTACTGTA GGAAAATAGC CTTAAGTTAT 
GCTCATTCCA CAGACAGTAA TTCCATTGGG GAAGACTTGA ATGCTCTTTC AGATTCAACT 
TGCTCTGTGT CTATACTTGA TCCAAGCGAA CCTCGGACAC CTGTTGGGAG TAGAAAAGCC 
AGTCGTAACA TATTCTTAGA GGATGATTTA GCCTGGCAAA GTTTGATTCA TCCGGATTCC 
TCAAATAGTG CTCTTTCAAC AAGACTCGTA TCTGTTCAAG AGGATGCTGG GAAGTCTCCT 
GCTCGGAACA GATCAGCCAG CATTACTAAT CTGTCACTGG ATCGGTCTGG TTCTCCTATG 
GTTCCTTCAT ATGAGACATC TGTCAGTCCC CAGGCTAACC GAAACTACAT TAGGACCGAG 
ACGACTGAGG ATGAACGCAA AATTCTTCTG GACAGTGCTC AGTTAAAGGA TCTGTGGAAG 
AAAATCTGCC ATCACACCAG TGGGATGGAA TTTCAAGATC ACCGTTACTG GTTGAGAACA 
CATCCCAACT GCATTGTAGG AAAGGAATTA GTCAACTGGC TAATCAGAAA TGGACACATC 
GCTACAAGGG CACAAGCTAT AGCAATTGGA CAAGCAATGG TTGATGGACG TTGGTTGGAT 
TGTGTTAGTC ATCATGACCA GCTTTTCAGG GACGAATATG CGTTGTATAG ACCACTTCAG 
AGTACAGAAT TTTCTGAGAC ACCTTCTCCA GACAGTGACT CCGTGAACTC TGTGGAAGGA 
CACTCCGAGC CATCCTGGTT TAAAGACATA AAATTTGATG ACAGTGACAC AGAACAGATT 
GCTGAAGAAG GTGACGATAA TTTGGCTAAT TCTGCCAGTC CTAGCAAGCG CACCTCAGTC 
AGCAGTTTCC AGTCCACAGT GGACAGTGAC TCAGCCGCTT CTATCAGCCT GAACGTGGAG 
CTGGACAACG TGAACTTCCA TATCAAGAAG CCCTCCAAGT ACCCACATGT GCCCCCTCAC 
CCTGCTGACC AAAAAGAGTA TTTGGTTTCT GACACTGGAG GACAGCAGCT CTCAATAAGT 
GATGCCTTCA TCAAAGAGTC CTTATTTAAT CGACGAGTAG AGGAAAAATC CAAAGAGCTG 
CCTTTTACCC CTTTGGGCTG GCATCATAAC AACCTGGAAC TCTTGCGAGA GGAGAATGAG 
GAGAAGCAAG CCATGGAAAG GCTGCTTTCA GCTAATCATA ACCACATGAT GGCCCTACTC 
CAGCAATTGC TTCAAAACGA GTCATTGTCA TCGTCTTGGA GGGACATCAT TGTGTCACTA 
GTCTGCCAGG TTGTTCAGAC AGTCCGACCT GACGTCAAGC ACCAGGATGA TGACATGGAT 
ATCCGTCAGT TTGTCCATAT CAAGAAGATC CCAGGTGGAA AGAAATTTGA TTCTGTGGTT 
GTCAATGGCT TTGTTTGTAC CAAGAACATT GCACATAAAA AGATGAATTC CTGTATTAAA 
AACCCCAAAA TCCTTCTGTT GAAGTGTTCT ATTGAGTATC TCTATAGAGA AGAAACTAAG 
TTTACCTGCA TTGATCCTAT TGTGCTTCAG GAAAGGGAAT TCTTGAAGAA TTATGTTCAA 
CGAATAGTTG ATGTTCGACC CACATTGGTT CTTGTTGAGA AAACCGTGTC TCGGATTGCT 
CAGGACATGT TACTGGAACA TGGCATTACT CTGGTCATTA ATGTAAAGTC ACAAGTTTTA 
GAAAGAATCA GTCGAATGAC CCAAGGTGAT TTAGTGGTGT CCATGGACCA GCTGCTCACC 
AAACCCCACT TGGGCACTTG CCACAAATTT TATATGCAGA TATTTCAGCT GCCTAATGAA 
CAAACCAAAA CACTGATGTT TTTTGAAGGC TGTCCACAGC ACCTAGGCTG CACAATCAAG 
CTCAGAGGAG GCTCTGATTA TGAGCTGGCT CGAGTTAAGG AGATCCTAAT ATTTATGATC 
TGTGTAGCTT ATCATTCTCA GCTAGAAATC TCTTTTCTCA TGGATGAGTT CGCTATGCCT 
CCAACGTTAA TGCAAAGCCC TTCGTTCCAT CTTCTGACGG AGGGACGGGG TGAAGAGGGA 
GCCTCTCAGG AGCAGGTCAG TGGCAGCTCC CTTCCTCAGG ATCCTGAGTG CCCTCGTGAG 
GCCCTGTCTT CTGACGATAG CACTTTGTTG GAATCAAGGA CTGTGCTAGA GAAGGGTGAA 
CTAGACAATA AAAGTATTCC ACAAGCTGTT GCCTCTTTGA AGCATCAAGA TTATACCACC 
CCCACTTGCC CAGCAGGTAT CCCCTGTGCT CTCTTTGCAT TGGTACCAGA GTCATTGTTG 
CCTCTCCATA TGGATCAACA GGATGCTGTA GGAAATGAAC AACCAGAGAC ATCACAACAA 
ACAGATGAGC AACAGGATCC CAAAAGCCAG ATGAAAGCTT TTAGAGACCC TCTACAGGAT 
GACACCGGAA TGTATGTTAC TGAGGAAGTC ACCTCCTCTG AAGATCAACG AAAGACTTAT 
GCCTTGACAT TTAAACAGGA ATTAAAAGAC GTAATCCTCT GTATCTCTCC AGTTATTACA 
TTCCGTGAAC CTTTCCTTTT AACTGAAAAG GGGATGAGAT GCTCAACTCG AGATTATTTT 
CCAGAGCAGA TTTACTGGTC TCCTCTTCTC AACAAAGAGG TGATGGAAAT GGAGAGCAGG 
AGGAAGAAAC AGCTGCTCAG GGATCTCTCT GGACTTCAGG GCATGAATGG CAGTGTTCAG 
GCCAAGTCTA TTCAAGTCTT ACCCTCACAT GAACTAGTGA GCACCAGGAT TGCTGAACAT 
CTGGGTGACA GCCAGACCTT GGGTAGAATG CTAGCTGATT ATCGAGCTAG AGGAGGAAGA 
ATTCAGTCAA AACATTTGGA CCCTTTTGTC CATTCAAAAG ATGCATCATG TACTTCAGGT 
GGAAAACCAG GAAACAAAAC TGAGAGTGAT GAAGAGAGGG GATTGATCCC AAGTGATGTA 
ATATGGCCAG CAAAGGTGGA CTGTCTGAAT CCTGCTAACC ACCAGAGGCT CTGTGTGCTC 
TTCAGCAGCT CTTCTGCCCA GTCCAGCAAT GCTCCCAGTG CTTGTGTCAG TCCTTGGATT 
GTAACAATGG AGTTTTATGG AAAGAATGAC CTTACACTGG GAATATTTTT AGAAAGATAC 
TGTTTCAGGT CTTCTTACCA GTGTCCGAGC ATGTTCTGTG ACACCCCCAT GGTTCATCAC 
ATTCGACGCT TTGTTCATGG CCAAGGCTGT GTACAGATAA TTCTGAAGGA GTTGGATTCT 
CCAGTGCCTG GATATCAACA TACAATTCTC ACATACTCCT GGTGCAGAAT CTGCAAACAA 
GTAACACCAG TTGTTGCTCT TTCAAATGAA TCCTGGTCTA TGTCATTTGC AAAGTACCTT 
GAACTTCGAT TTTATGGCCA CCAGTACACA CGCAGAGCCA ACGCTGAGCC CTGCGGTCAC 
TCTATCCACC ATGATTATCA CCAGTATTTC TCTTATAACC AGATGGTGGC ATCTTTCAGT 
TACTCTCCTA TTCGGCTTCT TGAAGTATGT GTTCCACTAC CAAAAATATT CATTAAGCGT 
CAAGCCCCAC TGAAGGTATC TCTTCTTCAG GACCTCAAGG ACTTTTTTCA GAAGGTTTCA 
CAGGTGTACC TAGCTGTTGA TGAGAGACTT GCATCCTTGA AAACGGATAC ATTTAGCAAA 
ACCAGAGAGG AAAAGATGGA AGATATCTTT GCACAAAAGG AGATGGAAGA GGGTGAGTTT 
AAGAACTGGA CAGAGAAGAT GCAAGCAAGG CTCATGTCTT CCTCTGTGGA TACCCCTCAG 
CAACTGCAGT CCATTTTTGA GTCACTGATT GCCAAGAAGC AAAGCCTCTG TGAGGTGCTC 
CAGGCGTGGA ACAGCAGGTT GCAGGACCTC TTCCAGCAGG AAAAAGGTAG AAAGAGGCCT 
TCAGTTCCTC CCAGTCCTGG GAGACTGAGA CAAGGTGAAG AAAGCAAGAT AAATGCAATG 
GACACATCTC CAAGGAATAT TTCTCCAGGA CTTCACAATG GAGAAAAAGA GGATCGCTTC 
TTGACAACCC TGTCCAGCCA GAGCTCTACG AGCTCCACCC ACCTCCAGCT GCCCACTCCT 
CCCGAGGCCC TGGCCGAGCA GGTAGTGGGA GGGCCGACTG ATCTGGATTC AGCCAGTGGT 
TCTGAAGATG TATTTGATGG TCATTTGCTG GGATCCACAG ACAGCCAGGT GAAGGAAAAG 
TCAACCATGA AAGCCATCTT TGCTAATTTG CTTCCAGGAA ACAGCTACAA TCCCATTCCA 
TTTCCTTTTG ATCCAGATAA ACACTACTTA ATGTATGAAC ATGAACGGGT GCCCATTGCT 
GTCTGTGAGA AAGAGCCCAG CTCCATCATT GCTTTTGCAC TCAGTTGTAA AGAATACCGA 
AATGCCTTAG AGGAATTGTC CAAAGCGACT CTGTGGAACA GTGCTGAAGA AGGGCTTCCA 
GCCAATAGTG CTTTAGATAA CAGACCTAAG AGTAGCAGCC CTATTAGAGT ACCTGAAATC 
AGTGGAGGAC AGACAAACCG CACAGGAGAA GCAGAACCTC AGCCAACCAA AAAAGCTTCA 
GGCATGTTGT CCTTCTTCAG AGGAACAGCA GGGAAGAGTC CTGACCTGTC TTCTCAGAAG 
AGGGAGACCT TACGTGGGGC AGACAGTGCT TACTACCAGG TTGGGCAGGC TGGCAAGGAG 
GGGTTGGAGA GTCAAGGCCT GGAGCCTCAA GATGAAGTAG ACGGAGGAGA TACACAGAAG 
AAACAACTCA CAAATCCTCA TGTGGAACTT CAATTTTCTG ATGCTAATGC CAAGTTTTAC 
TGTCGGCTGT ACTACGCGGG AGAGTTCCAT AAGATGCGTG AAGTGATTCT GGGCAGCAGC 
GAGGAGGATT TCATTCGCTC CCTTTCTCAC TCATCTCCCT GGCAGGCCCG GGGAGGCAAG 
TCAGGAGCTG CTTTCTATGC CACCGAAGAT GATAGATTCA TTCTGAAGCA AATGCCTCGT 
TTGGAAGTCC AGTCTTTCCT TGACTTTGCA CCACACTACT TCAATTATAT CACAAATGCT 
GTTCAACAAA AGAGGCCCAC CGCCTTGGCT AAAATTCTTG GAGTTTACAG AATTGGCTAT 
AAGAACTCTC AGAACAACAC TGAGAAGAAG TTAGATCTCC TTGTTATGGA AAATCTTTTC 
TATGGGAGAA AGATGGCACA GGTTTTTGAT TTGAAGGGTT CACTTAGGAA TCGAAATGTA 
AAAACTGACA CTGGGAAAGA GAGCTGTGAT GTGGTTCTGT TGGATGAAAA CCTCCTAAAG 
ATGGTTCGAG ACAACCCTCT CTATATTCGT TCCCATTCCA AATCTGTGCT GAGAACCTCC 
ATCCACAGCG ACGCCCATTT CCTTTCCAGC CACCTCATTA TAGACTATTC TCTGCTGGTT 
GGGCGAGATG ACACTAGCAA TGAGCTTGTG GTTGGCATCA TAGATTACAT TCGAACATTT 
ACATGGGACA AAAAACTTGA GATGGTTGTG AAGTCAACAG GAATTTTAGG AGGACAAGGT 
AAAATGCCAA CTGTGGTCTC TCCAGAGTTG TATAGGACTA GATTTTGTGA AGCAATGGAC 
AAGTATTTCT TGATGGTGCC AGACCACTGG ACAGGGTTGG ATCTGAATTG CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Pikfyve ( XM_021160793.1 ) cDNA ORF clone, Mus caroli(Ryukyu mouse) -> XP_021016452.1 Mus caroli phosphoinositide kinase, FYVE-type zinc finger containing (Pikfyve), transcript variant X1, mRNA.

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CloneID	OMv23070
Clone ID Related Accession (Same CDS sequence)	XM_021160793.2 , XM_021160800.1 , XM_021160793.1 , XM_021160800.2
Accession Version	XM_021160793.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6294bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-05-20
Organism	Mus caroli(Ryukyu mouse)
Product	1-phosphatidylinositol 3-phosphate 5-kinase
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_034570.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus caroli Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCACAG ATGACAAGAG TTCCCCGACA CTGGACTCTG CTAATGATTT GCCTCGCTCT 
CCCGCCAGTC CTTCTCACCT CACTCACTTT AAACCCTTGA CTCCTGACCA GGATGAGCCC 
CCCTTCAAGT CAGCATATAG TTCTTTTGTA AACCTTTTTC GCTTTAACAA AGAGCGGGGA 
GAAGGGGGCC AAGGAGAGCA GCAGTCTCCG AGTTCAAGTT GGGCCAGCCC TCAGATCCCT 
TCAAGAACAC AGTCTGTGAG GTCGCCTGTA CCTTATAAAA AACAGCTTAA TGAGGAGCTC 
CACCGGCGCT CTTCAGTGTT AGACTCCAGA AGGAAAGCAG AACCAGCCTG TGGAGGTCAT 
GACCCACGTA CAGCTGTTCA GCTTCGAAGC CTCAGCACAG TATTGAAACG CCTCAAAGAA 
ATCATGGAAG GAAAAAGCCA GGACAGTGAC CTGAAGCAAT ATTGGATGCC AGATAGCCAG 
TGTAAAGAGT GCTATGACTG CAGTGAAAAG TTTACAACAT TTAGGCGCAG ACACCATTGC 
AGACTGTGTG GGCAGATTTT CTGCAGTCGT TGTTGTAATC AAGAAATCCC TGGAAAATTT 
ATGGGCTATA CAGGAGACCT CCGAGCATGC ACCTACTGTA GGAAAATAGC CTTAAGTTAT 
GCTCATTCCA CAGACAGTAA TTCCATTGGG GAAGACTTGA ATGCTCTTTC AGATTCAACT 
TGCTCTGTGT CTATACTTGA TCCAAGCGAA CCTCGGACAC CTGTTGGGAG TAGAAAAGCC 
AGTCGTAACA TATTCTTAGA GGATGATTTA GCCTGGCAAA GTTTGATTCA TCCGGATTCC 
TCAAATAGTG CTCTTTCAAC AAGACTCGTA TCTGTTCAAG AGGATGCTGG GAAGTCTCCT 
GCTCGGAACA GATCAGCCAG CATTACTAAT CTGTCACTGG ATCGGTCTGG TTCTCCTATG 
GTTCCTTCAT ATGAGACATC TGTCAGTCCC CAGGCTAACC GAAACTACAT TAGGACCGAG 
ACGACTGAGG ATGAACGCAA AATTCTTCTG GACAGTGCTC AGTTAAAGGA TCTGTGGAAG 
AAAATCTGCC ATCACACCAG TGGGATGGAA TTTCAAGATC ACCGTTACTG GTTGAGAACA 
CATCCCAACT GCATTGTAGG AAAGGAATTA GTCAACTGGC TAATCAGAAA TGGACACATC 
GCTACAAGGG CACAAGCTAT AGCAATTGGA CAAGCAATGG TTGATGGACG TTGGTTGGAT 
TGTGTTAGTC ATCATGACCA GCTTTTCAGG GACGAATATG CGTTGTATAG ACCACTTCAG 
AGTACAGAAT TTTCTGAGAC ACCTTCTCCA GACAGTGACT CCGTGAACTC TGTGGAAGGA 
CACTCCGAGC CATCCTGGTT TAAAGACATA AAATTTGATG ACAGTGACAC AGAACAGATT 
GCTGAAGAAG GTGACGATAA TTTGGCTAAT TCTGCCAGTC CTAGCAAGCG CACCTCAGTC 
AGCAGTTTCC AGTCCACAGT GGACAGTGAC TCAGCCGCTT CTATCAGCCT GAACGTGGAG 
CTGGACAACG TGAACTTCCA TATCAAGAAG CCCTCCAAGT ACCCACATGT GCCCCCTCAC 
CCTGCTGACC AAAAAGAGTA TTTGGTTTCT GACACTGGAG GACAGCAGCT CTCAATAAGT 
GATGCCTTCA TCAAAGAGTC CTTATTTAAT CGACGAGTAG AGGAAAAATC CAAAGAGCTG 
CCTTTTACCC CTTTGGGCTG GCATCATAAC AACCTGGAAC TCTTGCGAGA GGAGAATGAG 
GAGAAGCAAG CCATGGAAAG GCTGCTTTCA GCTAATCATA ACCACATGAT GGCCCTACTC 
CAGCAATTGC TTCAAAACGA GTCATTGTCA TCGTCTTGGA GGGACATCAT TGTGTCACTA 
GTCTGCCAGG TTGTTCAGAC AGTCCGACCT GACGTCAAGC ACCAGGATGA TGACATGGAT 
ATCCGTCAGT TTGTCCATAT CAAGAAGATC CCAGGTGGAA AGAAATTTGA TTCTGTGGTT 
GTCAATGGCT TTGTTTGTAC CAAGAACATT GCACATAAAA AGATGAATTC CTGTATTAAA 
AACCCCAAAA TCCTTCTGTT GAAGTGTTCT ATTGAGTATC TCTATAGAGA AGAAACTAAG 
TTTACCTGCA TTGATCCTAT TGTGCTTCAG GAAAGGGAAT TCTTGAAGAA TTATGTTCAA 
CGAATAGTTG ATGTTCGACC CACATTGGTT CTTGTTGAGA AAACCGTGTC TCGGATTGCT 
CAGGACATGT TACTGGAACA TGGCATTACT CTGGTCATTA ATGTAAAGTC ACAAGTTTTA 
GAAAGAATCA GTCGAATGAC CCAAGGTGAT TTAGTGGTGT CCATGGACCA GCTGCTCACC 
AAACCCCACT TGGGCACTTG CCACAAATTT TATATGCAGA TATTTCAGCT GCCTAATGAA 
CAAACCAAAA CACTGATGTT TTTTGAAGGC TGTCCACAGC ACCTAGGCTG CACAATCAAG 
CTCAGAGGAG GCTCTGATTA TGAGCTGGCT CGAGTTAAGG AGATCCTAAT ATTTATGATC 
TGTGTAGCTT ATCATTCTCA GCTAGAAATC TCTTTTCTCA TGGATGAGTT CGCTATGCCT 
CCAACGTTAA TGCAAAGCCC TTCGTTCCAT CTTCTGACGG AGGGACGGGG TGAAGAGGGA 
GCCTCTCAGG AGCAGGTCAG TGGCAGCTCC CTTCCTCAGG ATCCTGAGTG CCCTCGTGAG 
GCCCTGTCTT CTGACGATAG CACTTTGTTG GAATCAAGGA CTGTGCTAGA GAAGGGTGAA 
CTAGACAATA AAAGTATTCC ACAAGCTGTT GCCTCTTTGA AGCATCAAGA TTATACCACC 
CCCACTTGCC CAGCAGGTAT CCCCTGTGCT CTCTTTGCAT TGGTACCAGA GTCATTGTTG 
CCTCTCCATA TGGATCAACA GGATGCTGTA GGAAATGAAC AACCAGAGAC ATCACAACAA 
ACAGATGAGC AACAGGATCC CAAAAGCCAG ATGAAAGCTT TTAGAGACCC TCTACAGGAT 
GACACCGGAA TGTATGTTAC TGAGGAAGTC ACCTCCTCTG AAGATCAACG AAAGACTTAT 
GCCTTGACAT TTAAACAGGA ATTAAAAGAC GTAATCCTCT GTATCTCTCC AGTTATTACA 
TTCCGTGAAC CTTTCCTTTT AACTGAAAAG GGGATGAGAT GCTCAACTCG AGATTATTTT 
CCAGAGCAGA TTTACTGGTC TCCTCTTCTC AACAAAGAGG TGATGGAAAT GGAGAGCAGG 
AGGAAGAAAC AGCTGCTCAG GGATCTCTCT GGACTTCAGG GCATGAATGG CAGTGTTCAG 
GCCAAGTCTA TTCAAGTCTT ACCCTCACAT GAACTAGTGA GCACCAGGAT TGCTGAACAT 
CTGGGTGACA GCCAGACCTT GGGTAGAATG CTAGCTGATT ATCGAGCTAG AGGAGGAAGA 
ATTCAGTCAA AACATTTGGA CCCTTTTGTC CATTCAAAAG ATGCATCATG TACTTCAGGT 
GGAAAACCAG GAAACAAAAC TGAGAGTGAT GAAGAGAGGG GATTGATCCC AAGTGATGTA 
ATATGGCCAG CAAAGGTGGA CTGTCTGAAT CCTGCTAACC ACCAGAGGCT CTGTGTGCTC 
TTCAGCAGCT CTTCTGCCCA GTCCAGCAAT GCTCCCAGTG CTTGTGTCAG TCCTTGGATT 
GTAACAATGG AGTTTTATGG AAAGAATGAC CTTACACTGG GAATATTTTT AGAAAGATAC 
TGTTTCAGGT CTTCTTACCA GTGTCCGAGC ATGTTCTGTG ACACCCCCAT GGTTCATCAC 
ATTCGACGCT TTGTTCATGG CCAAGGCTGT GTACAGATAA TTCTGAAGGA GTTGGATTCT 
CCAGTGCCTG GATATCAACA TACAATTCTC ACATACTCCT GGTGCAGAAT CTGCAAACAA 
GTAACACCAG TTGTTGCTCT TTCAAATGAA TCCTGGTCTA TGTCATTTGC AAAGTACCTT 
GAACTTCGAT TTTATGGCCA CCAGTACACA CGCAGAGCCA ACGCTGAGCC CTGCGGTCAC 
TCTATCCACC ATGATTATCA CCAGTATTTC TCTTATAACC AGATGGTGGC ATCTTTCAGT 
TACTCTCCTA TTCGGCTTCT TGAAGTATGT GTTCCACTAC CAAAAATATT CATTAAGCGT 
CAAGCCCCAC TGAAGGTATC TCTTCTTCAG GACCTCAAGG ACTTTTTTCA GAAGGTTTCA 
CAGGTGTACC TAGCTGTTGA TGAGAGACTT GCATCCTTGA AAACGGATAC ATTTAGCAAA 
ACCAGAGAGG AAAAGATGGA AGATATCTTT GCACAAAAGG AGATGGAAGA GGGTGAGTTT 
AAGAACTGGA CAGAGAAGAT GCAAGCAAGG CTCATGTCTT CCTCTGTGGA TACCCCTCAG 
CAACTGCAGT CCATTTTTGA GTCACTGATT GCCAAGAAGC AAAGCCTCTG TGAGGTGCTC 
CAGGCGTGGA ACAGCAGGTT GCAGGACCTC TTCCAGCAGG AAAAAGGTAG AAAGAGGCCT 
TCAGTTCCTC CCAGTCCTGG GAGACTGAGA CAAGGTGAAG AAAGCAAGAT AAATGCAATG 
GACACATCTC CAAGGAATAT TTCTCCAGGA CTTCACAATG GAGAAAAAGA GGATCGCTTC 
TTGACAACCC TGTCCAGCCA GAGCTCTACG AGCTCCACCC ACCTCCAGCT GCCCACTCCT 
CCCGAGGCCC TGGCCGAGCA GGTAGTGGGA GGGCCGACTG ATCTGGATTC AGCCAGTGGT 
TCTGAAGATG TATTTGATGG TCATTTGCTG GGATCCACAG ACAGCCAGGT GAAGGAAAAG 
TCAACCATGA AAGCCATCTT TGCTAATTTG CTTCCAGGAA ACAGCTACAA TCCCATTCCA 
TTTCCTTTTG ATCCAGATAA ACACTACTTA ATGTATGAAC ATGAACGGGT GCCCATTGCT 
GTCTGTGAGA AAGAGCCCAG CTCCATCATT GCTTTTGCAC TCAGTTGTAA AGAATACCGA 
AATGCCTTAG AGGAATTGTC CAAAGCGACT CTGTGGAACA GTGCTGAAGA AGGGCTTCCA 
GCCAATAGTG CTTTAGATAA CAGACCTAAG AGTAGCAGCC CTATTAGAGT ACCTGAAATC 
AGTGGAGGAC AGACAAACCG CACAGGAGAA GCAGAACCTC AGCCAACCAA AAAAGCTTCA 
GGCATGTTGT CCTTCTTCAG AGGAACAGCA GGGAAGAGTC CTGACCTGTC TTCTCAGAAG 
AGGGAGACCT TACGTGGGGC AGACAGTGCT TACTACCAGG TTGGGCAGGC TGGCAAGGAG 
GGGTTGGAGA GTCAAGGCCT GGAGCCTCAA GATGAAGTAG ACGGAGGAGA TACACAGAAG 
AAACAACTCA CAAATCCTCA TGTGGAACTT CAATTTTCTG ATGCTAATGC CAAGTTTTAC 
TGTCGGCTGT ACTACGCGGG AGAGTTCCAT AAGATGCGTG AAGTGATTCT GGGCAGCAGC 
GAGGAGGATT TCATTCGCTC CCTTTCTCAC TCATCTCCCT GGCAGGCCCG GGGAGGCAAG 
TCAGGAGCTG CTTTCTATGC CACCGAAGAT GATAGATTCA TTCTGAAGCA AATGCCTCGT 
TTGGAAGTCC AGTCTTTCCT TGACTTTGCA CCACACTACT TCAATTATAT CACAAATGCT 
GTTCAACAAA AGAGGCCCAC CGCCTTGGCT AAAATTCTTG GAGTTTACAG AATTGGCTAT 
AAGAACTCTC AGAACAACAC TGAGAAGAAG TTAGATCTCC TTGTTATGGA AAATCTTTTC 
TATGGGAGAA AGATGGCACA GGTTTTTGAT TTGAAGGGTT CACTTAGGAA TCGAAATGTA 
AAAACTGACA CTGGGAAAGA GAGCTGTGAT GTGGTTCTGT TGGATGAAAA CCTCCTAAAG 
ATGGTTCGAG ACAACCCTCT CTATATTCGT TCCCATTCCA AATCTGTGCT GAGAACCTCC 
ATCCACAGCG ACGCCCATTT CCTTTCCAGC CACCTCATTA TAGACTATTC TCTGCTGGTT 
GGGCGAGATG ACACTAGCAA TGAGCTTGTG GTTGGCATCA TAGATTACAT TCGAACATTT 
ACATGGGACA AAAAACTTGA GATGGTTGTG AAGTCAACAG GAATTTTAGG AGGACAAGGT 
AAAATGCCAA CTGTGGTCTC TCCAGAGTTG TATAGGACTA GATTTTGTGA AGCAATGGAC 
AAGTATTTCT TGATGGTGCC AGACCACTGG ACAGGGTTGG ATCTGAATTG CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021016452.1
CDS	151..6444
Translation	MATDDKSSPTLDSANDLPRSPASPSHLTHFKPLTPDQDEPPFKSAYSSFVNLFRFNKERGEGGQGEQQSPSSSWASPQIPSRTQSVRSPVPYKKQLNEELHRRSSVLDSRRKAEPACGGHDPRTAVQLRSLSTVLKRLKEIMEGKSQDSDLKQYWMPDSQCKECYDCSEKFTTFRRRHHCRLCGQIFCSRCCNQEIPGKFMGYTGDLRACTYCRKIALSYAHSTDSNSIGEDLNALSDSTCSVSILDPSEPRTPVGSRKASRNIFLEDDLAWQSLIHPDSSNSALSTRLVSVQEDAGKSPARNRSASITNLSLDRSGSPMVPSYETSVSPQANRNYIRTETTEDERKILLDSAQLKDLWKKICHHTSGMEFQDHRYWLRTHPNCIVGKELVNWLIRNGHIATRAQAIAIGQAMVDGRWLDCVSHHDQLFRDEYALYRPLQSTEFSETPSPDSDSVNSVEGHSEPSWFKDIKFDDSDTEQIAEEGDDNLANSASPSKRTSVSSFQSTVDSDSAASISLNVELDNVNFHIKKPSKYPHVPPHPADQKEYLVSDTGGQQLSISDAFIKESLFNRRVEEKSKELPFTPLGWHHNNLELLREENEEKQAMERLLSANHNHMMALLQQLLQNESLSSSWRDIIVSLVCQVVQTVRPDVKHQDDDMDIRQFVHIKKIPGGKKFDSVVVNGFVCTKNIAHKKMNSCIKNPKILLLKCSIEYLYREETKFTCIDPIVLQEREFLKNYVQRIVDVRPTLVLVEKTVSRIAQDMLLEHGITLVINVKSQVLERISRMTQGDLVVSMDQLLTKPHLGTCHKFYMQIFQLPNEQTKTLMFFEGCPQHLGCTIKLRGGSDYELARVKEILIFMICVAYHSQLEISFLMDEFAMPPTLMQSPSFHLLTEGRGEEGASQEQVSGSSLPQDPECPREALSSDDSTLLESRTVLEKGELDNKSIPQAVASLKHQDYTTPTCPAGIPCALFALVPESLLPLHMDQQDAVGNEQPETSQQTDEQQDPKSQMKAFRDPLQDDTGMYVTEEVTSSEDQRKTYALTFKQELKDVILCISPVITFREPFLLTEKGMRCSTRDYFPEQIYWSPLLNKEVMEMESRRKKQLLRDLSGLQGMNGSVQAKSIQVLPSHELVSTRIAEHLGDSQTLGRMLADYRARGGRIQSKHLDPFVHSKDASCTSGGKPGNKTESDEERGLIPSDVIWPAKVDCLNPANHQRLCVLFSSSSAQSSNAPSACVSPWIVTMEFYGKNDLTLGIFLERYCFRSSYQCPSMFCDTPMVHHIRRFVHGQGCVQIILKELDSPVPGYQHTILTYSWCRICKQVTPVVALSNESWSMSFAKYLELRFYGHQYTRRANAEPCGHSIHHDYHQYFSYNQMVASFSYSPIRLLEVCVPLPKIFIKRQAPLKVSLLQDLKDFFQKVSQVYLAVDERLASLKTDTFSKTREEKMEDIFAQKEMEEGEFKNWTEKMQARLMSSSVDTPQQLQSIFESLIAKKQSLCEVLQAWNSRLQDLFQQEKGRKRPSVPPSPGRLRQGEESKINAMDTSPRNISPGLHNGEKEDRFLTTLSSQSSTSSTHLQLPTPPEALAEQVVGGPTDLDSASGSEDVFDGHLLGSTDSQVKEKSTMKAIFANLLPGNSYNPIPFPFDPDKHYLMYEHERVPIAVCEKEPSSIIAFALSCKEYRNALEELSKATLWNSAEEGLPANSALDNRPKSSSPIRVPEISGGQTNRTGEAEPQPTKKASGMLSFFRGTAGKSPDLSSQKRETLRGADSAYYQVGQAGKEGLESQGLEPQDEVDGGDTQKKQLTNPHVELQFSDANAKFYCRLYYAGEFHKMREVILGSSEEDFIRSLSHSSPWQARGGKSGAAFYATEDDRFILKQMPRLEVQSFLDFAPHYFNYITNAVQQKRPTALAKILGVYRIGYKNSQNNTEKKLDLLVMENLFYGRKMAQVFDLKGSLRNRNVKTDTGKESCDVVLLDENLLKMVRDNPLYIRSHSKSVLRTSIHSDAHFLSSHLIIDYSLLVGRDDTSNELVVGIIDYIRTFTWDKKLEMVVKSTGILGGQGKMPTVVSPELYRTRFCEAMDKYFLMVPDHWTGLDLNC

Target ORF information:

RefSeq Version	XM_021160793.1
Organism	Mus caroli(Ryukyu mouse)
Definition	Mus caroli phosphoinositide kinase, FYVE-type zinc finger containing (Pikfyve), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021160793.1

ORF Insert Sequence:

ATGGCCACAG ATGACAAGAG TTCCCCGACA CTGGACTCTG CTAATGATTT GCCTCGCTCT 
CCCGCCAGTC CTTCTCACCT CACTCACTTT AAACCCTTGA CTCCTGACCA GGATGAGCCC 
CCCTTCAAGT CAGCATATAG TTCTTTTGTA AACCTTTTTC GCTTTAACAA AGAGCGGGGA 
GAAGGGGGCC AAGGAGAGCA GCAGTCTCCG AGTTCAAGTT GGGCCAGCCC TCAGATCCCT 
TCAAGAACAC AGTCTGTGAG GTCGCCTGTA CCTTATAAAA AACAGCTTAA TGAGGAGCTC 
CACCGGCGCT CTTCAGTGTT AGACTCCAGA AGGAAAGCAG AACCAGCCTG TGGAGGTCAT 
GACCCACGTA CAGCTGTTCA GCTTCGAAGC CTCAGCACAG TATTGAAACG CCTCAAAGAA 
ATCATGGAAG GAAAAAGCCA GGACAGTGAC CTGAAGCAAT ATTGGATGCC AGATAGCCAG 
TGTAAAGAGT GCTATGACTG CAGTGAAAAG TTTACAACAT TTAGGCGCAG ACACCATTGC 
AGACTGTGTG GGCAGATTTT CTGCAGTCGT TGTTGTAATC AAGAAATCCC TGGAAAATTT 
ATGGGCTATA CAGGAGACCT CCGAGCATGC ACCTACTGTA GGAAAATAGC CTTAAGTTAT 
GCTCATTCCA CAGACAGTAA TTCCATTGGG GAAGACTTGA ATGCTCTTTC AGATTCAACT 
TGCTCTGTGT CTATACTTGA TCCAAGCGAA CCTCGGACAC CTGTTGGGAG TAGAAAAGCC 
AGTCGTAACA TATTCTTAGA GGATGATTTA GCCTGGCAAA GTTTGATTCA TCCGGATTCC 
TCAAATAGTG CTCTTTCAAC AAGACTCGTA TCTGTTCAAG AGGATGCTGG GAAGTCTCCT 
GCTCGGAACA GATCAGCCAG CATTACTAAT CTGTCACTGG ATCGGTCTGG TTCTCCTATG 
GTTCCTTCAT ATGAGACATC TGTCAGTCCC CAGGCTAACC GAAACTACAT TAGGACCGAG 
ACGACTGAGG ATGAACGCAA AATTCTTCTG GACAGTGCTC AGTTAAAGGA TCTGTGGAAG 
AAAATCTGCC ATCACACCAG TGGGATGGAA TTTCAAGATC ACCGTTACTG GTTGAGAACA 
CATCCCAACT GCATTGTAGG AAAGGAATTA GTCAACTGGC TAATCAGAAA TGGACACATC 
GCTACAAGGG CACAAGCTAT AGCAATTGGA CAAGCAATGG TTGATGGACG TTGGTTGGAT 
TGTGTTAGTC ATCATGACCA GCTTTTCAGG GACGAATATG CGTTGTATAG ACCACTTCAG 
AGTACAGAAT TTTCTGAGAC ACCTTCTCCA GACAGTGACT CCGTGAACTC TGTGGAAGGA 
CACTCCGAGC CATCCTGGTT TAAAGACATA AAATTTGATG ACAGTGACAC AGAACAGATT 
GCTGAAGAAG GTGACGATAA TTTGGCTAAT TCTGCCAGTC CTAGCAAGCG CACCTCAGTC 
AGCAGTTTCC AGTCCACAGT GGACAGTGAC TCAGCCGCTT CTATCAGCCT GAACGTGGAG 
CTGGACAACG TGAACTTCCA TATCAAGAAG CCCTCCAAGT ACCCACATGT GCCCCCTCAC 
CCTGCTGACC AAAAAGAGTA TTTGGTTTCT GACACTGGAG GACAGCAGCT CTCAATAAGT 
GATGCCTTCA TCAAAGAGTC CTTATTTAAT CGACGAGTAG AGGAAAAATC CAAAGAGCTG 
CCTTTTACCC CTTTGGGCTG GCATCATAAC AACCTGGAAC TCTTGCGAGA GGAGAATGAG 
GAGAAGCAAG CCATGGAAAG GCTGCTTTCA GCTAATCATA ACCACATGAT GGCCCTACTC 
CAGCAATTGC TTCAAAACGA GTCATTGTCA TCGTCTTGGA GGGACATCAT TGTGTCACTA 
GTCTGCCAGG TTGTTCAGAC AGTCCGACCT GACGTCAAGC ACCAGGATGA TGACATGGAT 
ATCCGTCAGT TTGTCCATAT CAAGAAGATC CCAGGTGGAA AGAAATTTGA TTCTGTGGTT 
GTCAATGGCT TTGTTTGTAC CAAGAACATT GCACATAAAA AGATGAATTC CTGTATTAAA 
AACCCCAAAA TCCTTCTGTT GAAGTGTTCT ATTGAGTATC TCTATAGAGA AGAAACTAAG 
TTTACCTGCA TTGATCCTAT TGTGCTTCAG GAAAGGGAAT TCTTGAAGAA TTATGTTCAA 
CGAATAGTTG ATGTTCGACC CACATTGGTT CTTGTTGAGA AAACCGTGTC TCGGATTGCT 
CAGGACATGT TACTGGAACA TGGCATTACT CTGGTCATTA ATGTAAAGTC ACAAGTTTTA 
GAAAGAATCA GTCGAATGAC CCAAGGTGAT TTAGTGGTGT CCATGGACCA GCTGCTCACC 
AAACCCCACT TGGGCACTTG CCACAAATTT TATATGCAGA TATTTCAGCT GCCTAATGAA 
CAAACCAAAA CACTGATGTT TTTTGAAGGC TGTCCACAGC ACCTAGGCTG CACAATCAAG 
CTCAGAGGAG GCTCTGATTA TGAGCTGGCT CGAGTTAAGG AGATCCTAAT ATTTATGATC 
TGTGTAGCTT ATCATTCTCA GCTAGAAATC TCTTTTCTCA TGGATGAGTT CGCTATGCCT 
CCAACGTTAA TGCAAAGCCC TTCGTTCCAT CTTCTGACGG AGGGACGGGG TGAAGAGGGA 
GCCTCTCAGG AGCAGGTCAG TGGCAGCTCC CTTCCTCAGG ATCCTGAGTG CCCTCGTGAG 
GCCCTGTCTT CTGACGATAG CACTTTGTTG GAATCAAGGA CTGTGCTAGA GAAGGGTGAA 
CTAGACAATA AAAGTATTCC ACAAGCTGTT GCCTCTTTGA AGCATCAAGA TTATACCACC 
CCCACTTGCC CAGCAGGTAT CCCCTGTGCT CTCTTTGCAT TGGTACCAGA GTCATTGTTG 
CCTCTCCATA TGGATCAACA GGATGCTGTA GGAAATGAAC AACCAGAGAC ATCACAACAA 
ACAGATGAGC AACAGGATCC CAAAAGCCAG ATGAAAGCTT TTAGAGACCC TCTACAGGAT 
GACACCGGAA TGTATGTTAC TGAGGAAGTC ACCTCCTCTG AAGATCAACG AAAGACTTAT 
GCCTTGACAT TTAAACAGGA ATTAAAAGAC GTAATCCTCT GTATCTCTCC AGTTATTACA 
TTCCGTGAAC CTTTCCTTTT AACTGAAAAG GGGATGAGAT GCTCAACTCG AGATTATTTT 
CCAGAGCAGA TTTACTGGTC TCCTCTTCTC AACAAAGAGG TGATGGAAAT GGAGAGCAGG 
AGGAAGAAAC AGCTGCTCAG GGATCTCTCT GGACTTCAGG GCATGAATGG CAGTGTTCAG 
GCCAAGTCTA TTCAAGTCTT ACCCTCACAT GAACTAGTGA GCACCAGGAT TGCTGAACAT 
CTGGGTGACA GCCAGACCTT GGGTAGAATG CTAGCTGATT ATCGAGCTAG AGGAGGAAGA 
ATTCAGTCAA AACATTTGGA CCCTTTTGTC CATTCAAAAG ATGCATCATG TACTTCAGGT 
GGAAAACCAG GAAACAAAAC TGAGAGTGAT GAAGAGAGGG GATTGATCCC AAGTGATGTA 
ATATGGCCAG CAAAGGTGGA CTGTCTGAAT CCTGCTAACC ACCAGAGGCT CTGTGTGCTC 
TTCAGCAGCT CTTCTGCCCA GTCCAGCAAT GCTCCCAGTG CTTGTGTCAG TCCTTGGATT 
GTAACAATGG AGTTTTATGG AAAGAATGAC CTTACACTGG GAATATTTTT AGAAAGATAC 
TGTTTCAGGT CTTCTTACCA GTGTCCGAGC ATGTTCTGTG ACACCCCCAT GGTTCATCAC 
ATTCGACGCT TTGTTCATGG CCAAGGCTGT GTACAGATAA TTCTGAAGGA GTTGGATTCT 
CCAGTGCCTG GATATCAACA TACAATTCTC ACATACTCCT GGTGCAGAAT CTGCAAACAA 
GTAACACCAG TTGTTGCTCT TTCAAATGAA TCCTGGTCTA TGTCATTTGC AAAGTACCTT 
GAACTTCGAT TTTATGGCCA CCAGTACACA CGCAGAGCCA ACGCTGAGCC CTGCGGTCAC 
TCTATCCACC ATGATTATCA CCAGTATTTC TCTTATAACC AGATGGTGGC ATCTTTCAGT 
TACTCTCCTA TTCGGCTTCT TGAAGTATGT GTTCCACTAC CAAAAATATT CATTAAGCGT 
CAAGCCCCAC TGAAGGTATC TCTTCTTCAG GACCTCAAGG ACTTTTTTCA GAAGGTTTCA 
CAGGTGTACC TAGCTGTTGA TGAGAGACTT GCATCCTTGA AAACGGATAC ATTTAGCAAA 
ACCAGAGAGG AAAAGATGGA AGATATCTTT GCACAAAAGG AGATGGAAGA GGGTGAGTTT 
AAGAACTGGA CAGAGAAGAT GCAAGCAAGG CTCATGTCTT CCTCTGTGGA TACCCCTCAG 
CAACTGCAGT CCATTTTTGA GTCACTGATT GCCAAGAAGC AAAGCCTCTG TGAGGTGCTC 
CAGGCGTGGA ACAGCAGGTT GCAGGACCTC TTCCAGCAGG AAAAAGGTAG AAAGAGGCCT 
TCAGTTCCTC CCAGTCCTGG GAGACTGAGA CAAGGTGAAG AAAGCAAGAT AAATGCAATG 
GACACATCTC CAAGGAATAT TTCTCCAGGA CTTCACAATG GAGAAAAAGA GGATCGCTTC 
TTGACAACCC TGTCCAGCCA GAGCTCTACG AGCTCCACCC ACCTCCAGCT GCCCACTCCT 
CCCGAGGCCC TGGCCGAGCA GGTAGTGGGA GGGCCGACTG ATCTGGATTC AGCCAGTGGT 
TCTGAAGATG TATTTGATGG TCATTTGCTG GGATCCACAG ACAGCCAGGT GAAGGAAAAG 
TCAACCATGA AAGCCATCTT TGCTAATTTG CTTCCAGGAA ACAGCTACAA TCCCATTCCA 
TTTCCTTTTG ATCCAGATAA ACACTACTTA ATGTATGAAC ATGAACGGGT GCCCATTGCT 
GTCTGTGAGA AAGAGCCCAG CTCCATCATT GCTTTTGCAC TCAGTTGTAA AGAATACCGA 
AATGCCTTAG AGGAATTGTC CAAAGCGACT CTGTGGAACA GTGCTGAAGA AGGGCTTCCA 
GCCAATAGTG CTTTAGATAA CAGACCTAAG AGTAGCAGCC CTATTAGAGT ACCTGAAATC 
AGTGGAGGAC AGACAAACCG CACAGGAGAA GCAGAACCTC AGCCAACCAA AAAAGCTTCA 
GGCATGTTGT CCTTCTTCAG AGGAACAGCA GGGAAGAGTC CTGACCTGTC TTCTCAGAAG 
AGGGAGACCT TACGTGGGGC AGACAGTGCT TACTACCAGG TTGGGCAGGC TGGCAAGGAG 
GGGTTGGAGA GTCAAGGCCT GGAGCCTCAA GATGAAGTAG ACGGAGGAGA TACACAGAAG 
AAACAACTCA CAAATCCTCA TGTGGAACTT CAATTTTCTG ATGCTAATGC CAAGTTTTAC 
TGTCGGCTGT ACTACGCGGG AGAGTTCCAT AAGATGCGTG AAGTGATTCT GGGCAGCAGC 
GAGGAGGATT TCATTCGCTC CCTTTCTCAC TCATCTCCCT GGCAGGCCCG GGGAGGCAAG 
TCAGGAGCTG CTTTCTATGC CACCGAAGAT GATAGATTCA TTCTGAAGCA AATGCCTCGT 
TTGGAAGTCC AGTCTTTCCT TGACTTTGCA CCACACTACT TCAATTATAT CACAAATGCT 
GTTCAACAAA AGAGGCCCAC CGCCTTGGCT AAAATTCTTG GAGTTTACAG AATTGGCTAT 
AAGAACTCTC AGAACAACAC TGAGAAGAAG TTAGATCTCC TTGTTATGGA AAATCTTTTC 
TATGGGAGAA AGATGGCACA GGTTTTTGAT TTGAAGGGTT CACTTAGGAA TCGAAATGTA 
AAAACTGACA CTGGGAAAGA GAGCTGTGAT GTGGTTCTGT TGGATGAAAA CCTCCTAAAG 
ATGGTTCGAG ACAACCCTCT CTATATTCGT TCCCATTCCA AATCTGTGCT GAGAACCTCC 
ATCCACAGCG ACGCCCATTT CCTTTCCAGC CACCTCATTA TAGACTATTC TCTGCTGGTT 
GGGCGAGATG ACACTAGCAA TGAGCTTGTG GTTGGCATCA TAGATTACAT TCGAACATTT 
ACATGGGACA AAAAACTTGA GATGGTTGTG AAGTCAACAG GAATTTTAGG AGGACAAGGT 
AAAATGCCAA CTGTGGTCTC TCCAGAGTTG TATAGGACTA GATTTTGTGA AGCAATGGAC 
AAGTATTTCT TGATGGTGCC AGACCACTGG ACAGGGTTGG ATCTGAATTG CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Pikfyve ( XM_021160800.2 ) cDNA ORF clone, Mus caroli(Ryukyu mouse) -> XP_021016459.1 Mus caroli phosphoinositide kinase, FYVE-type zinc finger containing (Pikfyve), transcript variant X5, mRNA.

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CloneID	OMv23070
Clone ID Related Accession (Same CDS sequence)	XM_021160793.2 , XM_021160800.1 , XM_021160793.1 , XM_021160800.2
Accession Version	XM_021160800.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6294bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-06-09
Organism	Mus caroli(Ryukyu mouse)
Product	1-phosphatidylinositol 3-phosphate 5-kinase isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_034570.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process On Jun 10, 2019 this sequence version replaced XM_021160800.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus caroli Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCACAG ATGACAAGAG TTCCCCGACA CTGGACTCTG CTAATGATTT GCCTCGCTCT 
CCCGCCAGTC CTTCTCACCT CACTCACTTT AAACCCTTGA CTCCTGACCA GGATGAGCCC 
CCCTTCAAGT CAGCATATAG TTCTTTTGTA AACCTTTTTC GCTTTAACAA AGAGCGGGGA 
GAAGGGGGCC AAGGAGAGCA GCAGTCTCCG AGTTCAAGTT GGGCCAGCCC TCAGATCCCT 
TCAAGAACAC AGTCTGTGAG GTCGCCTGTA CCTTATAAAA AACAGCTTAA TGAGGAGCTC 
CACCGGCGCT CTTCAGTGTT AGACTCCAGA AGGAAAGCAG AACCAGCCTG TGGAGGTCAT 
GACCCACGTA CAGCTGTTCA GCTTCGAAGC CTCAGCACAG TATTGAAACG CCTCAAAGAA 
ATCATGGAAG GAAAAAGCCA GGACAGTGAC CTGAAGCAAT ATTGGATGCC AGATAGCCAG 
TGTAAAGAGT GCTATGACTG CAGTGAAAAG TTTACAACAT TTAGGCGCAG ACACCATTGC 
AGACTGTGTG GGCAGATTTT CTGCAGTCGT TGTTGTAATC AAGAAATCCC TGGAAAATTT 
ATGGGCTATA CAGGAGACCT CCGAGCATGC ACCTACTGTA GGAAAATAGC CTTAAGTTAT 
GCTCATTCCA CAGACAGTAA TTCCATTGGG GAAGACTTGA ATGCTCTTTC AGATTCAACT 
TGCTCTGTGT CTATACTTGA TCCAAGCGAA CCTCGGACAC CTGTTGGGAG TAGAAAAGCC 
AGTCGTAACA TATTCTTAGA GGATGATTTA GCCTGGCAAA GTTTGATTCA TCCGGATTCC 
TCAAATAGTG CTCTTTCAAC AAGACTCGTA TCTGTTCAAG AGGATGCTGG GAAGTCTCCT 
GCTCGGAACA GATCAGCCAG CATTACTAAT CTGTCACTGG ATCGGTCTGG TTCTCCTATG 
GTTCCTTCAT ATGAGACATC TGTCAGTCCC CAGGCTAACC GAAACTACAT TAGGACCGAG 
ACGACTGAGG ATGAACGCAA AATTCTTCTG GACAGTGCTC AGTTAAAGGA TCTGTGGAAG 
AAAATCTGCC ATCACACCAG TGGGATGGAA TTTCAAGATC ACCGTTACTG GTTGAGAACA 
CATCCCAACT GCATTGTAGG AAAGGAATTA GTCAACTGGC TAATCAGAAA TGGACACATC 
GCTACAAGGG CACAAGCTAT AGCAATTGGA CAAGCAATGG TTGATGGACG TTGGTTGGAT 
TGTGTTAGTC ATCATGACCA GCTTTTCAGG GACGAATATG CGTTGTATAG ACCACTTCAG 
AGTACAGAAT TTTCTGAGAC ACCTTCTCCA GACAGTGACT CCGTGAACTC TGTGGAAGGA 
CACTCCGAGC CATCCTGGTT TAAAGACATA AAATTTGATG ACAGTGACAC AGAACAGATT 
GCTGAAGAAG GTGACGATAA TTTGGCTAAT TCTGCCAGTC CTAGCAAGCG CACCTCAGTC 
AGCAGTTTCC AGTCCACAGT GGACAGTGAC TCAGCCGCTT CTATCAGCCT GAACGTGGAG 
CTGGACAACG TGAACTTCCA TATCAAGAAG CCCTCCAAGT ACCCACATGT GCCCCCTCAC 
CCTGCTGACC AAAAAGAGTA TTTGGTTTCT GACACTGGAG GACAGCAGCT CTCAATAAGT 
GATGCCTTCA TCAAAGAGTC CTTATTTAAT CGACGAGTAG AGGAAAAATC CAAAGAGCTG 
CCTTTTACCC CTTTGGGCTG GCATCATAAC AACCTGGAAC TCTTGCGAGA GGAGAATGAG 
GAGAAGCAAG CCATGGAAAG GCTGCTTTCA GCTAATCATA ACCACATGAT GGCCCTACTC 
CAGCAATTGC TTCAAAACGA GTCATTGTCA TCGTCTTGGA GGGACATCAT TGTGTCACTA 
GTCTGCCAGG TTGTTCAGAC AGTCCGACCT GACGTCAAGC ACCAGGATGA TGACATGGAT 
ATCCGTCAGT TTGTCCATAT CAAGAAGATC CCAGGTGGAA AGAAATTTGA TTCTGTGGTT 
GTCAATGGCT TTGTTTGTAC CAAGAACATT GCACATAAAA AGATGAATTC CTGTATTAAA 
AACCCCAAAA TCCTTCTGTT GAAGTGTTCT ATTGAGTATC TCTATAGAGA AGAAACTAAG 
TTTACCTGCA TTGATCCTAT TGTGCTTCAG GAAAGGGAAT TCTTGAAGAA TTATGTTCAA 
CGAATAGTTG ATGTTCGACC CACATTGGTT CTTGTTGAGA AAACCGTGTC TCGGATTGCT 
CAGGACATGT TACTGGAACA TGGCATTACT CTGGTCATTA ATGTAAAGTC ACAAGTTTTA 
GAAAGAATCA GTCGAATGAC CCAAGGTGAT TTAGTGGTGT CCATGGACCA GCTGCTCACC 
AAACCCCACT TGGGCACTTG CCACAAATTT TATATGCAGA TATTTCAGCT GCCTAATGAA 
CAAACCAAAA CACTGATGTT TTTTGAAGGC TGTCCACAGC ACCTAGGCTG CACAATCAAG 
CTCAGAGGAG GCTCTGATTA TGAGCTGGCT CGAGTTAAGG AGATCCTAAT ATTTATGATC 
TGTGTAGCTT ATCATTCTCA GCTAGAAATC TCTTTTCTCA TGGATGAGTT CGCTATGCCT 
CCAACGTTAA TGCAAAGCCC TTCGTTCCAT CTTCTGACGG AGGGACGGGG TGAAGAGGGA 
GCCTCTCAGG AGCAGGTCAG TGGCAGCTCC CTTCCTCAGG ATCCTGAGTG CCCTCGTGAG 
GCCCTGTCTT CTGACGATAG CACTTTGTTG GAATCAAGGA CTGTGCTAGA GAAGGGTGAA 
CTAGACAATA AAAGTATTCC ACAAGCTGTT GCCTCTTTGA AGCATCAAGA TTATACCACC 
CCCACTTGCC CAGCAGGTAT CCCCTGTGCT CTCTTTGCAT TGGTACCAGA GTCATTGTTG 
CCTCTCCATA TGGATCAACA GGATGCTGTA GGAAATGAAC AACCAGAGAC ATCACAACAA 
ACAGATGAGC AACAGGATCC CAAAAGCCAG ATGAAAGCTT TTAGAGACCC TCTACAGGAT 
GACACCGGAA TGTATGTTAC TGAGGAAGTC ACCTCCTCTG AAGATCAACG AAAGACTTAT 
GCCTTGACAT TTAAACAGGA ATTAAAAGAC GTAATCCTCT GTATCTCTCC AGTTATTACA 
TTCCGTGAAC CTTTCCTTTT AACTGAAAAG GGGATGAGAT GCTCAACTCG AGATTATTTT 
CCAGAGCAGA TTTACTGGTC TCCTCTTCTC AACAAAGAGG TGATGGAAAT GGAGAGCAGG 
AGGAAGAAAC AGCTGCTCAG GGATCTCTCT GGACTTCAGG GCATGAATGG CAGTGTTCAG 
GCCAAGTCTA TTCAAGTCTT ACCCTCACAT GAACTAGTGA GCACCAGGAT TGCTGAACAT 
CTGGGTGACA GCCAGACCTT GGGTAGAATG CTAGCTGATT ATCGAGCTAG AGGAGGAAGA 
ATTCAGTCAA AACATTTGGA CCCTTTTGTC CATTCAAAAG ATGCATCATG TACTTCAGGT 
GGAAAACCAG GAAACAAAAC TGAGAGTGAT GAAGAGAGGG GATTGATCCC AAGTGATGTA 
ATATGGCCAG CAAAGGTGGA CTGTCTGAAT CCTGCTAACC ACCAGAGGCT CTGTGTGCTC 
TTCAGCAGCT CTTCTGCCCA GTCCAGCAAT GCTCCCAGTG CTTGTGTCAG TCCTTGGATT 
GTAACAATGG AGTTTTATGG AAAGAATGAC CTTACACTGG GAATATTTTT AGAAAGATAC 
TGTTTCAGGT CTTCTTACCA GTGTCCGAGC ATGTTCTGTG ACACCCCCAT GGTTCATCAC 
ATTCGACGCT TTGTTCATGG CCAAGGCTGT GTACAGATAA TTCTGAAGGA GTTGGATTCT 
CCAGTGCCTG GATATCAACA TACAATTCTC ACATACTCCT GGTGCAGAAT CTGCAAACAA 
GTAACACCAG TTGTTGCTCT TTCAAATGAA TCCTGGTCTA TGTCATTTGC AAAGTACCTT 
GAACTTCGAT TTTATGGCCA CCAGTACACA CGCAGAGCCA ACGCTGAGCC CTGCGGTCAC 
TCTATCCACC ATGATTATCA CCAGTATTTC TCTTATAACC AGATGGTGGC ATCTTTCAGT 
TACTCTCCTA TTCGGCTTCT TGAAGTATGT GTTCCACTAC CAAAAATATT CATTAAGCGT 
CAAGCCCCAC TGAAGGTATC TCTTCTTCAG GACCTCAAGG ACTTTTTTCA GAAGGTTTCA 
CAGGTGTACC TAGCTGTTGA TGAGAGACTT GCATCCTTGA AAACGGATAC ATTTAGCAAA 
ACCAGAGAGG AAAAGATGGA AGATATCTTT GCACAAAAGG AGATGGAAGA GGGTGAGTTT 
AAGAACTGGA CAGAGAAGAT GCAAGCAAGG CTCATGTCTT CCTCTGTGGA TACCCCTCAG 
CAACTGCAGT CCATTTTTGA GTCACTGATT GCCAAGAAGC AAAGCCTCTG TGAGGTGCTC 
CAGGCGTGGA ACAGCAGGTT GCAGGACCTC TTCCAGCAGG AAAAAGGTAG AAAGAGGCCT 
TCAGTTCCTC CCAGTCCTGG GAGACTGAGA CAAGGTGAAG AAAGCAAGAT AAATGCAATG 
GACACATCTC CAAGGAATAT TTCTCCAGGA CTTCACAATG GAGAAAAAGA GGATCGCTTC 
TTGACAACCC TGTCCAGCCA GAGCTCTACG AGCTCCACCC ACCTCCAGCT GCCCACTCCT 
CCCGAGGCCC TGGCCGAGCA GGTAGTGGGA GGGCCGACTG ATCTGGATTC AGCCAGTGGT 
TCTGAAGATG TATTTGATGG TCATTTGCTG GGATCCACAG ACAGCCAGGT GAAGGAAAAG 
TCAACCATGA AAGCCATCTT TGCTAATTTG CTTCCAGGAA ACAGCTACAA TCCCATTCCA 
TTTCCTTTTG ATCCAGATAA ACACTACTTA ATGTATGAAC ATGAACGGGT GCCCATTGCT 
GTCTGTGAGA AAGAGCCCAG CTCCATCATT GCTTTTGCAC TCAGTTGTAA AGAATACCGA 
AATGCCTTAG AGGAATTGTC CAAAGCGACT CTGTGGAACA GTGCTGAAGA AGGGCTTCCA 
GCCAATAGTG CTTTAGATAA CAGACCTAAG AGTAGCAGCC CTATTAGAGT ACCTGAAATC 
AGTGGAGGAC AGACAAACCG CACAGGAGAA GCAGAACCTC AGCCAACCAA AAAAGCTTCA 
GGCATGTTGT CCTTCTTCAG AGGAACAGCA GGGAAGAGTC CTGACCTGTC TTCTCAGAAG 
AGGGAGACCT TACGTGGGGC AGACAGTGCT TACTACCAGG TTGGGCAGGC TGGCAAGGAG 
GGGTTGGAGA GTCAAGGCCT GGAGCCTCAA GATGAAGTAG ACGGAGGAGA TACACAGAAG 
AAACAACTCA CAAATCCTCA TGTGGAACTT CAATTTTCTG ATGCTAATGC CAAGTTTTAC 
TGTCGGCTGT ACTACGCGGG AGAGTTCCAT AAGATGCGTG AAGTGATTCT GGGCAGCAGC 
GAGGAGGATT TCATTCGCTC CCTTTCTCAC TCATCTCCCT GGCAGGCCCG GGGAGGCAAG 
TCAGGAGCTG CTTTCTATGC CACCGAAGAT GATAGATTCA TTCTGAAGCA AATGCCTCGT 
TTGGAAGTCC AGTCTTTCCT TGACTTTGCA CCACACTACT TCAATTATAT CACAAATGCT 
GTTCAACAAA AGAGGCCCAC CGCCTTGGCT AAAATTCTTG GAGTTTACAG AATTGGCTAT 
AAGAACTCTC AGAACAACAC TGAGAAGAAG TTAGATCTCC TTGTTATGGA AAATCTTTTC 
TATGGGAGAA AGATGGCACA GGTTTTTGAT TTGAAGGGTT CACTTAGGAA TCGAAATGTA 
AAAACTGACA CTGGGAAAGA GAGCTGTGAT GTGGTTCTGT TGGATGAAAA CCTCCTAAAG 
ATGGTTCGAG ACAACCCTCT CTATATTCGT TCCCATTCCA AATCTGTGCT GAGAACCTCC 
ATCCACAGCG ACGCCCATTT CCTTTCCAGC CACCTCATTA TAGACTATTC TCTGCTGGTT 
GGGCGAGATG ACACTAGCAA TGAGCTTGTG GTTGGCATCA TAGATTACAT TCGAACATTT 
ACATGGGACA AAAAACTTGA GATGGTTGTG AAGTCAACAG GAATTTTAGG AGGACAAGGT 
AAAATGCCAA CTGTGGTCTC TCCAGAGTTG TATAGGACTA GATTTTGTGA AGCAATGGAC 
AAGTATTTCT TGATGGTGCC AGACCACTGG ACAGGGTTGG ATCTGAATTG CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021016459.1
CDS	101..6394
Translation	MATDDKSSPTLDSANDLPRSPASPSHLTHFKPLTPDQDEPPFKSAYSSFVNLFRFNKERGEGGQGEQQSPSSSWASPQIPSRTQSVRSPVPYKKQLNEELHRRSSVLDSRRKAEPACGGHDPRTAVQLRSLSTVLKRLKEIMEGKSQDSDLKQYWMPDSQCKECYDCSEKFTTFRRRHHCRLCGQIFCSRCCNQEIPGKFMGYTGDLRACTYCRKIALSYAHSTDSNSIGEDLNALSDSTCSVSILDPSEPRTPVGSRKASRNIFLEDDLAWQSLIHPDSSNSALSTRLVSVQEDAGKSPARNRSASITNLSLDRSGSPMVPSYETSVSPQANRNYIRTETTEDERKILLDSAQLKDLWKKICHHTSGMEFQDHRYWLRTHPNCIVGKELVNWLIRNGHIATRAQAIAIGQAMVDGRWLDCVSHHDQLFRDEYALYRPLQSTEFSETPSPDSDSVNSVEGHSEPSWFKDIKFDDSDTEQIAEEGDDNLANSASPSKRTSVSSFQSTVDSDSAASISLNVELDNVNFHIKKPSKYPHVPPHPADQKEYLVSDTGGQQLSISDAFIKESLFNRRVEEKSKELPFTPLGWHHNNLELLREENEEKQAMERLLSANHNHMMALLQQLLQNESLSSSWRDIIVSLVCQVVQTVRPDVKHQDDDMDIRQFVHIKKIPGGKKFDSVVVNGFVCTKNIAHKKMNSCIKNPKILLLKCSIEYLYREETKFTCIDPIVLQEREFLKNYVQRIVDVRPTLVLVEKTVSRIAQDMLLEHGITLVINVKSQVLERISRMTQGDLVVSMDQLLTKPHLGTCHKFYMQIFQLPNEQTKTLMFFEGCPQHLGCTIKLRGGSDYELARVKEILIFMICVAYHSQLEISFLMDEFAMPPTLMQSPSFHLLTEGRGEEGASQEQVSGSSLPQDPECPREALSSDDSTLLESRTVLEKGELDNKSIPQAVASLKHQDYTTPTCPAGIPCALFALVPESLLPLHMDQQDAVGNEQPETSQQTDEQQDPKSQMKAFRDPLQDDTGMYVTEEVTSSEDQRKTYALTFKQELKDVILCISPVITFREPFLLTEKGMRCSTRDYFPEQIYWSPLLNKEVMEMESRRKKQLLRDLSGLQGMNGSVQAKSIQVLPSHELVSTRIAEHLGDSQTLGRMLADYRARGGRIQSKHLDPFVHSKDASCTSGGKPGNKTESDEERGLIPSDVIWPAKVDCLNPANHQRLCVLFSSSSAQSSNAPSACVSPWIVTMEFYGKNDLTLGIFLERYCFRSSYQCPSMFCDTPMVHHIRRFVHGQGCVQIILKELDSPVPGYQHTILTYSWCRICKQVTPVVALSNESWSMSFAKYLELRFYGHQYTRRANAEPCGHSIHHDYHQYFSYNQMVASFSYSPIRLLEVCVPLPKIFIKRQAPLKVSLLQDLKDFFQKVSQVYLAVDERLASLKTDTFSKTREEKMEDIFAQKEMEEGEFKNWTEKMQARLMSSSVDTPQQLQSIFESLIAKKQSLCEVLQAWNSRLQDLFQQEKGRKRPSVPPSPGRLRQGEESKINAMDTSPRNISPGLHNGEKEDRFLTTLSSQSSTSSTHLQLPTPPEALAEQVVGGPTDLDSASGSEDVFDGHLLGSTDSQVKEKSTMKAIFANLLPGNSYNPIPFPFDPDKHYLMYEHERVPIAVCEKEPSSIIAFALSCKEYRNALEELSKATLWNSAEEGLPANSALDNRPKSSSPIRVPEISGGQTNRTGEAEPQPTKKASGMLSFFRGTAGKSPDLSSQKRETLRGADSAYYQVGQAGKEGLESQGLEPQDEVDGGDTQKKQLTNPHVELQFSDANAKFYCRLYYAGEFHKMREVILGSSEEDFIRSLSHSSPWQARGGKSGAAFYATEDDRFILKQMPRLEVQSFLDFAPHYFNYITNAVQQKRPTALAKILGVYRIGYKNSQNNTEKKLDLLVMENLFYGRKMAQVFDLKGSLRNRNVKTDTGKESCDVVLLDENLLKMVRDNPLYIRSHSKSVLRTSIHSDAHFLSSHLIIDYSLLVGRDDTSNELVVGIIDYIRTFTWDKKLEMVVKSTGILGGQGKMPTVVSPELYRTRFCEAMDKYFLMVPDHWTGLDLNC

Target ORF information:

RefSeq Version	XM_021160800.2
Organism	Mus caroli(Ryukyu mouse)
Definition	Mus caroli phosphoinositide kinase, FYVE-type zinc finger containing (Pikfyve), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021160800.2

ORF Insert Sequence:

ATGGCCACAG ATGACAAGAG TTCCCCGACA CTGGACTCTG CTAATGATTT GCCTCGCTCT 
CCCGCCAGTC CTTCTCACCT CACTCACTTT AAACCCTTGA CTCCTGACCA GGATGAGCCC 
CCCTTCAAGT CAGCATATAG TTCTTTTGTA AACCTTTTTC GCTTTAACAA AGAGCGGGGA 
GAAGGGGGCC AAGGAGAGCA GCAGTCTCCG AGTTCAAGTT GGGCCAGCCC TCAGATCCCT 
TCAAGAACAC AGTCTGTGAG GTCGCCTGTA CCTTATAAAA AACAGCTTAA TGAGGAGCTC 
CACCGGCGCT CTTCAGTGTT AGACTCCAGA AGGAAAGCAG AACCAGCCTG TGGAGGTCAT 
GACCCACGTA CAGCTGTTCA GCTTCGAAGC CTCAGCACAG TATTGAAACG CCTCAAAGAA 
ATCATGGAAG GAAAAAGCCA GGACAGTGAC CTGAAGCAAT ATTGGATGCC AGATAGCCAG 
TGTAAAGAGT GCTATGACTG CAGTGAAAAG TTTACAACAT TTAGGCGCAG ACACCATTGC 
AGACTGTGTG GGCAGATTTT CTGCAGTCGT TGTTGTAATC AAGAAATCCC TGGAAAATTT 
ATGGGCTATA CAGGAGACCT CCGAGCATGC ACCTACTGTA GGAAAATAGC CTTAAGTTAT 
GCTCATTCCA CAGACAGTAA TTCCATTGGG GAAGACTTGA ATGCTCTTTC AGATTCAACT 
TGCTCTGTGT CTATACTTGA TCCAAGCGAA CCTCGGACAC CTGTTGGGAG TAGAAAAGCC 
AGTCGTAACA TATTCTTAGA GGATGATTTA GCCTGGCAAA GTTTGATTCA TCCGGATTCC 
TCAAATAGTG CTCTTTCAAC AAGACTCGTA TCTGTTCAAG AGGATGCTGG GAAGTCTCCT 
GCTCGGAACA GATCAGCCAG CATTACTAAT CTGTCACTGG ATCGGTCTGG TTCTCCTATG 
GTTCCTTCAT ATGAGACATC TGTCAGTCCC CAGGCTAACC GAAACTACAT TAGGACCGAG 
ACGACTGAGG ATGAACGCAA AATTCTTCTG GACAGTGCTC AGTTAAAGGA TCTGTGGAAG 
AAAATCTGCC ATCACACCAG TGGGATGGAA TTTCAAGATC ACCGTTACTG GTTGAGAACA 
CATCCCAACT GCATTGTAGG AAAGGAATTA GTCAACTGGC TAATCAGAAA TGGACACATC 
GCTACAAGGG CACAAGCTAT AGCAATTGGA CAAGCAATGG TTGATGGACG TTGGTTGGAT 
TGTGTTAGTC ATCATGACCA GCTTTTCAGG GACGAATATG CGTTGTATAG ACCACTTCAG 
AGTACAGAAT TTTCTGAGAC ACCTTCTCCA GACAGTGACT CCGTGAACTC TGTGGAAGGA 
CACTCCGAGC CATCCTGGTT TAAAGACATA AAATTTGATG ACAGTGACAC AGAACAGATT 
GCTGAAGAAG GTGACGATAA TTTGGCTAAT TCTGCCAGTC CTAGCAAGCG CACCTCAGTC 
AGCAGTTTCC AGTCCACAGT GGACAGTGAC TCAGCCGCTT CTATCAGCCT GAACGTGGAG 
CTGGACAACG TGAACTTCCA TATCAAGAAG CCCTCCAAGT ACCCACATGT GCCCCCTCAC 
CCTGCTGACC AAAAAGAGTA TTTGGTTTCT GACACTGGAG GACAGCAGCT CTCAATAAGT 
GATGCCTTCA TCAAAGAGTC CTTATTTAAT CGACGAGTAG AGGAAAAATC CAAAGAGCTG 
CCTTTTACCC CTTTGGGCTG GCATCATAAC AACCTGGAAC TCTTGCGAGA GGAGAATGAG 
GAGAAGCAAG CCATGGAAAG GCTGCTTTCA GCTAATCATA ACCACATGAT GGCCCTACTC 
CAGCAATTGC TTCAAAACGA GTCATTGTCA TCGTCTTGGA GGGACATCAT TGTGTCACTA 
GTCTGCCAGG TTGTTCAGAC AGTCCGACCT GACGTCAAGC ACCAGGATGA TGACATGGAT 
ATCCGTCAGT TTGTCCATAT CAAGAAGATC CCAGGTGGAA AGAAATTTGA TTCTGTGGTT 
GTCAATGGCT TTGTTTGTAC CAAGAACATT GCACATAAAA AGATGAATTC CTGTATTAAA 
AACCCCAAAA TCCTTCTGTT GAAGTGTTCT ATTGAGTATC TCTATAGAGA AGAAACTAAG 
TTTACCTGCA TTGATCCTAT TGTGCTTCAG GAAAGGGAAT TCTTGAAGAA TTATGTTCAA 
CGAATAGTTG ATGTTCGACC CACATTGGTT CTTGTTGAGA AAACCGTGTC TCGGATTGCT 
CAGGACATGT TACTGGAACA TGGCATTACT CTGGTCATTA ATGTAAAGTC ACAAGTTTTA 
GAAAGAATCA GTCGAATGAC CCAAGGTGAT TTAGTGGTGT CCATGGACCA GCTGCTCACC 
AAACCCCACT TGGGCACTTG CCACAAATTT TATATGCAGA TATTTCAGCT GCCTAATGAA 
CAAACCAAAA CACTGATGTT TTTTGAAGGC TGTCCACAGC ACCTAGGCTG CACAATCAAG 
CTCAGAGGAG GCTCTGATTA TGAGCTGGCT CGAGTTAAGG AGATCCTAAT ATTTATGATC 
TGTGTAGCTT ATCATTCTCA GCTAGAAATC TCTTTTCTCA TGGATGAGTT CGCTATGCCT 
CCAACGTTAA TGCAAAGCCC TTCGTTCCAT CTTCTGACGG AGGGACGGGG TGAAGAGGGA 
GCCTCTCAGG AGCAGGTCAG TGGCAGCTCC CTTCCTCAGG ATCCTGAGTG CCCTCGTGAG 
GCCCTGTCTT CTGACGATAG CACTTTGTTG GAATCAAGGA CTGTGCTAGA GAAGGGTGAA 
CTAGACAATA AAAGTATTCC ACAAGCTGTT GCCTCTTTGA AGCATCAAGA TTATACCACC 
CCCACTTGCC CAGCAGGTAT CCCCTGTGCT CTCTTTGCAT TGGTACCAGA GTCATTGTTG 
CCTCTCCATA TGGATCAACA GGATGCTGTA GGAAATGAAC AACCAGAGAC ATCACAACAA 
ACAGATGAGC AACAGGATCC CAAAAGCCAG ATGAAAGCTT TTAGAGACCC TCTACAGGAT 
GACACCGGAA TGTATGTTAC TGAGGAAGTC ACCTCCTCTG AAGATCAACG AAAGACTTAT 
GCCTTGACAT TTAAACAGGA ATTAAAAGAC GTAATCCTCT GTATCTCTCC AGTTATTACA 
TTCCGTGAAC CTTTCCTTTT AACTGAAAAG GGGATGAGAT GCTCAACTCG AGATTATTTT 
CCAGAGCAGA TTTACTGGTC TCCTCTTCTC AACAAAGAGG TGATGGAAAT GGAGAGCAGG 
AGGAAGAAAC AGCTGCTCAG GGATCTCTCT GGACTTCAGG GCATGAATGG CAGTGTTCAG 
GCCAAGTCTA TTCAAGTCTT ACCCTCACAT GAACTAGTGA GCACCAGGAT TGCTGAACAT 
CTGGGTGACA GCCAGACCTT GGGTAGAATG CTAGCTGATT ATCGAGCTAG AGGAGGAAGA 
ATTCAGTCAA AACATTTGGA CCCTTTTGTC CATTCAAAAG ATGCATCATG TACTTCAGGT 
GGAAAACCAG GAAACAAAAC TGAGAGTGAT GAAGAGAGGG GATTGATCCC AAGTGATGTA 
ATATGGCCAG CAAAGGTGGA CTGTCTGAAT CCTGCTAACC ACCAGAGGCT CTGTGTGCTC 
TTCAGCAGCT CTTCTGCCCA GTCCAGCAAT GCTCCCAGTG CTTGTGTCAG TCCTTGGATT 
GTAACAATGG AGTTTTATGG AAAGAATGAC CTTACACTGG GAATATTTTT AGAAAGATAC 
TGTTTCAGGT CTTCTTACCA GTGTCCGAGC ATGTTCTGTG ACACCCCCAT GGTTCATCAC 
ATTCGACGCT TTGTTCATGG CCAAGGCTGT GTACAGATAA TTCTGAAGGA GTTGGATTCT 
CCAGTGCCTG GATATCAACA TACAATTCTC ACATACTCCT GGTGCAGAAT CTGCAAACAA 
GTAACACCAG TTGTTGCTCT TTCAAATGAA TCCTGGTCTA TGTCATTTGC AAAGTACCTT 
GAACTTCGAT TTTATGGCCA CCAGTACACA CGCAGAGCCA ACGCTGAGCC CTGCGGTCAC 
TCTATCCACC ATGATTATCA CCAGTATTTC TCTTATAACC AGATGGTGGC ATCTTTCAGT 
TACTCTCCTA TTCGGCTTCT TGAAGTATGT GTTCCACTAC CAAAAATATT CATTAAGCGT 
CAAGCCCCAC TGAAGGTATC TCTTCTTCAG GACCTCAAGG ACTTTTTTCA GAAGGTTTCA 
CAGGTGTACC TAGCTGTTGA TGAGAGACTT GCATCCTTGA AAACGGATAC ATTTAGCAAA 
ACCAGAGAGG AAAAGATGGA AGATATCTTT GCACAAAAGG AGATGGAAGA GGGTGAGTTT 
AAGAACTGGA CAGAGAAGAT GCAAGCAAGG CTCATGTCTT CCTCTGTGGA TACCCCTCAG 
CAACTGCAGT CCATTTTTGA GTCACTGATT GCCAAGAAGC AAAGCCTCTG TGAGGTGCTC 
CAGGCGTGGA ACAGCAGGTT GCAGGACCTC TTCCAGCAGG AAAAAGGTAG AAAGAGGCCT 
TCAGTTCCTC CCAGTCCTGG GAGACTGAGA CAAGGTGAAG AAAGCAAGAT AAATGCAATG 
GACACATCTC CAAGGAATAT TTCTCCAGGA CTTCACAATG GAGAAAAAGA GGATCGCTTC 
TTGACAACCC TGTCCAGCCA GAGCTCTACG AGCTCCACCC ACCTCCAGCT GCCCACTCCT 
CCCGAGGCCC TGGCCGAGCA GGTAGTGGGA GGGCCGACTG ATCTGGATTC AGCCAGTGGT 
TCTGAAGATG TATTTGATGG TCATTTGCTG GGATCCACAG ACAGCCAGGT GAAGGAAAAG 
TCAACCATGA AAGCCATCTT TGCTAATTTG CTTCCAGGAA ACAGCTACAA TCCCATTCCA 
TTTCCTTTTG ATCCAGATAA ACACTACTTA ATGTATGAAC ATGAACGGGT GCCCATTGCT 
GTCTGTGAGA AAGAGCCCAG CTCCATCATT GCTTTTGCAC TCAGTTGTAA AGAATACCGA 
AATGCCTTAG AGGAATTGTC CAAAGCGACT CTGTGGAACA GTGCTGAAGA AGGGCTTCCA 
GCCAATAGTG CTTTAGATAA CAGACCTAAG AGTAGCAGCC CTATTAGAGT ACCTGAAATC 
AGTGGAGGAC AGACAAACCG CACAGGAGAA GCAGAACCTC AGCCAACCAA AAAAGCTTCA 
GGCATGTTGT CCTTCTTCAG AGGAACAGCA GGGAAGAGTC CTGACCTGTC TTCTCAGAAG 
AGGGAGACCT TACGTGGGGC AGACAGTGCT TACTACCAGG TTGGGCAGGC TGGCAAGGAG 
GGGTTGGAGA GTCAAGGCCT GGAGCCTCAA GATGAAGTAG ACGGAGGAGA TACACAGAAG 
AAACAACTCA CAAATCCTCA TGTGGAACTT CAATTTTCTG ATGCTAATGC CAAGTTTTAC 
TGTCGGCTGT ACTACGCGGG AGAGTTCCAT AAGATGCGTG AAGTGATTCT GGGCAGCAGC 
GAGGAGGATT TCATTCGCTC CCTTTCTCAC TCATCTCCCT GGCAGGCCCG GGGAGGCAAG 
TCAGGAGCTG CTTTCTATGC CACCGAAGAT GATAGATTCA TTCTGAAGCA AATGCCTCGT 
TTGGAAGTCC AGTCTTTCCT TGACTTTGCA CCACACTACT TCAATTATAT CACAAATGCT 
GTTCAACAAA AGAGGCCCAC CGCCTTGGCT AAAATTCTTG GAGTTTACAG AATTGGCTAT 
AAGAACTCTC AGAACAACAC TGAGAAGAAG TTAGATCTCC TTGTTATGGA AAATCTTTTC 
TATGGGAGAA AGATGGCACA GGTTTTTGAT TTGAAGGGTT CACTTAGGAA TCGAAATGTA 
AAAACTGACA CTGGGAAAGA GAGCTGTGAT GTGGTTCTGT TGGATGAAAA CCTCCTAAAG 
ATGGTTCGAG ACAACCCTCT CTATATTCGT TCCCATTCCA AATCTGTGCT GAGAACCTCC 
ATCCACAGCG ACGCCCATTT CCTTTCCAGC CACCTCATTA TAGACTATTC TCTGCTGGTT 
GGGCGAGATG ACACTAGCAA TGAGCTTGTG GTTGGCATCA TAGATTACAT TCGAACATTT 
ACATGGGACA AAAAACTTGA GATGGTTGTG AAGTCAACAG GAATTTTAGG AGGACAAGGT 
AAAATGCCAA CTGTGGTCTC TCCAGAGTTG TATAGGACTA GATTTTGTGA AGCAATGGAC 
AAGTATTTCT TGATGGTGCC AGACCACTGG ACAGGGTTGG ATCTGAATTG CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Pikfyve ( XM_029476064.1 ) cDNA ORF clone, Mus caroli(Ryukyu mouse) -> XP_029331924.1 Mus caroli phosphoinositide kinase, FYVE-type zinc finger containing (Pikfyve), transcript variant X1, mRNA.

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CloneID	OMv66195
Clone ID Related Accession (Same CDS sequence)	XM_029476064.1 , XM_029476067.1
Accession Version	XM_029476064.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6330bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-06-09
Organism	Mus caroli(Ryukyu mouse)
Product	1-phosphatidylinositol 3-phosphate 5-kinase isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_034570.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus caroli Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCACAG ATGACAAGAG TTCCCCGACA CTGGACTCTG CTAATGATTT GCCTCGCTCT 
CCCGCCAGTC CTTCTCACCT CACTCACTTT AAACCCTTGA CTCCTGACCA GGATGAGCCC 
CCCTTCAAGT CAGCATATAG TTCTTTTGTA AACCTTTTTC GCTTTAACAA AGAGCGGGGA 
GAAGGGGGCC AAGGAGAGCA GCAGTCTCCG AGTTCAAGTT GGGCCAGCCC TCAGATCCCT 
TCAAGAACAC AGTCTGTGAG GTCGCCTGTA CCTTATAAAA AACAGCTTAA TGAGGAGCTC 
CACCGGCGCT CTTCAGTGTT AGCAGAGAAC ACTTTGCCAC ATCCTCAGGA GAGCACAGAC 
TCCAGAAGGA AAGCAGAACC AGCCTGTGGA GGTCATGACC CACGTACAGC TGTTCAGCTT 
CGAAGCCTCA GCACAGTATT GAAACGCCTC AAAGAAATCA TGGAAGGAAA AAGCCAGGAC 
AGTGACCTGA AGCAATATTG GATGCCAGAT AGCCAGTGTA AAGAGTGCTA TGACTGCAGT 
GAAAAGTTTA CAACATTTAG GCGCAGACAC CATTGCAGAC TGTGTGGGCA GATTTTCTGC 
AGTCGTTGTT GTAATCAAGA AATCCCTGGA AAATTTATGG GCTATACAGG AGACCTCCGA 
GCATGCACCT ACTGTAGGAA AATAGCCTTA AGTTATGCTC ATTCCACAGA CAGTAATTCC 
ATTGGGGAAG ACTTGAATGC TCTTTCAGAT TCAACTTGCT CTGTGTCTAT ACTTGATCCA 
AGCGAACCTC GGACACCTGT TGGGAGTAGA AAAGCCAGTC GTAACATATT CTTAGAGGAT 
GATTTAGCCT GGCAAAGTTT GATTCATCCG GATTCCTCAA ATAGTGCTCT TTCAACAAGA 
CTCGTATCTG TTCAAGAGGA TGCTGGGAAG TCTCCTGCTC GGAACAGATC AGCCAGCATT 
ACTAATCTGT CACTGGATCG GTCTGGTTCT CCTATGGTTC CTTCATATGA GACATCTGTC 
AGTCCCCAGG CTAACCGAAA CTACATTAGG ACCGAGACGA CTGAGGATGA ACGCAAAATT 
CTTCTGGACA GTGCTCAGTT AAAGGATCTG TGGAAGAAAA TCTGCCATCA CACCAGTGGG 
ATGGAATTTC AAGATCACCG TTACTGGTTG AGAACACATC CCAACTGCAT TGTAGGAAAG 
GAATTAGTCA ACTGGCTAAT CAGAAATGGA CACATCGCTA CAAGGGCACA AGCTATAGCA 
ATTGGACAAG CAATGGTTGA TGGACGTTGG TTGGATTGTG TTAGTCATCA TGACCAGCTT 
TTCAGGGACG AATATGCGTT GTATAGACCA CTTCAGAGTA CAGAATTTTC TGAGACACCT 
TCTCCAGACA GTGACTCCGT GAACTCTGTG GAAGGACACT CCGAGCCATC CTGGTTTAAA 
GACATAAAAT TTGATGACAG TGACACAGAA CAGATTGCTG AAGAAGGTGA CGATAATTTG 
GCTAATTCTG CCAGTCCTAG CAAGCGCACC TCAGTCAGCA GTTTCCAGTC CACAGTGGAC 
AGTGACTCAG CCGCTTCTAT CAGCCTGAAC GTGGAGCTGG ACAACGTGAA CTTCCATATC 
AAGAAGCCCT CCAAGTACCC ACATGTGCCC CCTCACCCTG CTGACCAAAA AGAGTATTTG 
GTTTCTGACA CTGGAGGACA GCAGCTCTCA ATAAGTGATG CCTTCATCAA AGAGTCCTTA 
TTTAATCGAC GAGTAGAGGA AAAATCCAAA GAGCTGCCTT TTACCCCTTT GGGCTGGCAT 
CATAACAACC TGGAACTCTT GCGAGAGGAG AATGAGGAGA AGCAAGCCAT GGAAAGGCTG 
CTTTCAGCTA ATCATAACCA CATGATGGCC CTACTCCAGC AATTGCTTCA AAACGAGTCA 
TTGTCATCGT CTTGGAGGGA CATCATTGTG TCACTAGTCT GCCAGGTTGT TCAGACAGTC 
CGACCTGACG TCAAGCACCA GGATGATGAC ATGGATATCC GTCAGTTTGT CCATATCAAG 
AAGATCCCAG GTGGAAAGAA ATTTGATTCT GTGGTTGTCA ATGGCTTTGT TTGTACCAAG 
AACATTGCAC ATAAAAAGAT GAATTCCTGT ATTAAAAACC CCAAAATCCT TCTGTTGAAG 
TGTTCTATTG AGTATCTCTA TAGAGAAGAA ACTAAGTTTA CCTGCATTGA TCCTATTGTG 
CTTCAGGAAA GGGAATTCTT GAAGAATTAT GTTCAACGAA TAGTTGATGT TCGACCCACA 
TTGGTTCTTG TTGAGAAAAC CGTGTCTCGG ATTGCTCAGG ACATGTTACT GGAACATGGC 
ATTACTCTGG TCATTAATGT AAAGTCACAA GTTTTAGAAA GAATCAGTCG AATGACCCAA 
GGTGATTTAG TGGTGTCCAT GGACCAGCTG CTCACCAAAC CCCACTTGGG CACTTGCCAC 
AAATTTTATA TGCAGATATT TCAGCTGCCT AATGAACAAA CCAAAACACT GATGTTTTTT 
GAAGGCTGTC CACAGCACCT AGGCTGCACA ATCAAGCTCA GAGGAGGCTC TGATTATGAG 
CTGGCTCGAG TTAAGGAGAT CCTAATATTT ATGATCTGTG TAGCTTATCA TTCTCAGCTA 
GAAATCTCTT TTCTCATGGA TGAGTTCGCT ATGCCTCCAA CGTTAATGCA AAGCCCTTCG 
TTCCATCTTC TGACGGAGGG ACGGGGTGAA GAGGGAGCCT CTCAGGAGCA GGTCAGTGGC 
AGCTCCCTTC CTCAGGATCC TGAGTGCCCT CGTGAGGCCC TGTCTTCTGA CGATAGCACT 
TTGTTGGAAT CAAGGACTGT GCTAGAGAAG GGTGAACTAG ACAATAAAAG TATTCCACAA 
GCTGTTGCCT CTTTGAAGCA TCAAGATTAT ACCACCCCCA CTTGCCCAGC AGGTATCCCC 
TGTGCTCTCT TTGCATTGGT ACCAGAGTCA TTGTTGCCTC TCCATATGGA TCAACAGGAT 
GCTGTAGGAA ATGAACAACC AGAGACATCA CAACAAACAG ATGAGCAACA GGATCCCAAA 
AGCCAGATGA AAGCTTTTAG AGACCCTCTA CAGGATGACA CCGGAATGTA TGTTACTGAG 
GAAGTCACCT CCTCTGAAGA TCAACGAAAG ACTTATGCCT TGACATTTAA ACAGGAATTA 
AAAGACGTAA TCCTCTGTAT CTCTCCAGTT ATTACATTCC GTGAACCTTT CCTTTTAACT 
GAAAAGGGGA TGAGATGCTC AACTCGAGAT TATTTTCCAG AGCAGATTTA CTGGTCTCCT 
CTTCTCAACA AAGAGGTGAT GGAAATGGAG AGCAGGAGGA AGAAACAGCT GCTCAGGGAT 
CTCTCTGGAC TTCAGGGCAT GAATGGCAGT GTTCAGGCCA AGTCTATTCA AGTCTTACCC 
TCACATGAAC TAGTGAGCAC CAGGATTGCT GAACATCTGG GTGACAGCCA GACCTTGGGT 
AGAATGCTAG CTGATTATCG AGCTAGAGGA GGAAGAATTC AGTCAAAACA TTTGGACCCT 
TTTGTCCATT CAAAAGATGC ATCATGTACT TCAGGTGGAA AACCAGGAAA CAAAACTGAG 
AGTGATGAAG AGAGGGGATT GATCCCAAGT GATGTAATAT GGCCAGCAAA GGTGGACTGT 
CTGAATCCTG CTAACCACCA GAGGCTCTGT GTGCTCTTCA GCAGCTCTTC TGCCCAGTCC 
AGCAATGCTC CCAGTGCTTG TGTCAGTCCT TGGATTGTAA CAATGGAGTT TTATGGAAAG 
AATGACCTTA CACTGGGAAT ATTTTTAGAA AGATACTGTT TCAGGTCTTC TTACCAGTGT 
CCGAGCATGT TCTGTGACAC CCCCATGGTT CATCACATTC GACGCTTTGT TCATGGCCAA 
GGCTGTGTAC AGATAATTCT GAAGGAGTTG GATTCTCCAG TGCCTGGATA TCAACATACA 
ATTCTCACAT ACTCCTGGTG CAGAATCTGC AAACAAGTAA CACCAGTTGT TGCTCTTTCA 
AATGAATCCT GGTCTATGTC ATTTGCAAAG TACCTTGAAC TTCGATTTTA TGGCCACCAG 
TACACACGCA GAGCCAACGC TGAGCCCTGC GGTCACTCTA TCCACCATGA TTATCACCAG 
TATTTCTCTT ATAACCAGAT GGTGGCATCT TTCAGTTACT CTCCTATTCG GCTTCTTGAA 
GTATGTGTTC CACTACCAAA AATATTCATT AAGCGTCAAG CCCCACTGAA GGTATCTCTT 
CTTCAGGACC TCAAGGACTT TTTTCAGAAG GTTTCACAGG TGTACCTAGC TGTTGATGAG 
AGACTTGCAT CCTTGAAAAC GGATACATTT AGCAAAACCA GAGAGGAAAA GATGGAAGAT 
ATCTTTGCAC AAAAGGAGAT GGAAGAGGGT GAGTTTAAGA ACTGGACAGA GAAGATGCAA 
GCAAGGCTCA TGTCTTCCTC TGTGGATACC CCTCAGCAAC TGCAGTCCAT TTTTGAGTCA 
CTGATTGCCA AGAAGCAAAG CCTCTGTGAG GTGCTCCAGG CGTGGAACAG CAGGTTGCAG 
GACCTCTTCC AGCAGGAAAA AGGTAGAAAG AGGCCTTCAG TTCCTCCCAG TCCTGGGAGA 
CTGAGACAAG GTGAAGAAAG CAAGATAAAT GCAATGGACA CATCTCCAAG GAATATTTCT 
CCAGGACTTC ACAATGGAGA AAAAGAGGAT CGCTTCTTGA CAACCCTGTC CAGCCAGAGC 
TCTACGAGCT CCACCCACCT CCAGCTGCCC ACTCCTCCCG AGGCCCTGGC CGAGCAGGTA 
GTGGGAGGGC CGACTGATCT GGATTCAGCC AGTGGTTCTG AAGATGTATT TGATGGTCAT 
TTGCTGGGAT CCACAGACAG CCAGGTGAAG GAAAAGTCAA CCATGAAAGC CATCTTTGCT 
AATTTGCTTC CAGGAAACAG CTACAATCCC ATTCCATTTC CTTTTGATCC AGATAAACAC 
TACTTAATGT ATGAACATGA ACGGGTGCCC ATTGCTGTCT GTGAGAAAGA GCCCAGCTCC 
ATCATTGCTT TTGCACTCAG TTGTAAAGAA TACCGAAATG CCTTAGAGGA ATTGTCCAAA 
GCGACTCTGT GGAACAGTGC TGAAGAAGGG CTTCCAGCCA ATAGTGCTTT AGATAACAGA 
CCTAAGAGTA GCAGCCCTAT TAGAGTACCT GAAATCAGTG GAGGACAGAC AAACCGCACA 
GGAGAAGCAG AACCTCAGCC AACCAAAAAA GCTTCAGGCA TGTTGTCCTT CTTCAGAGGA 
ACAGCAGGGA AGAGTCCTGA CCTGTCTTCT CAGAAGAGGG AGACCTTACG TGGGGCAGAC 
AGTGCTTACT ACCAGGTTGG GCAGGCTGGC AAGGAGGGGT TGGAGAGTCA AGGCCTGGAG 
CCTCAAGATG AAGTAGACGG AGGAGATACA CAGAAGAAAC AACTCACAAA TCCTCATGTG 
GAACTTCAAT TTTCTGATGC TAATGCCAAG TTTTACTGTC GGCTGTACTA CGCGGGAGAG 
TTCCATAAGA TGCGTGAAGT GATTCTGGGC AGCAGCGAGG AGGATTTCAT TCGCTCCCTT 
TCTCACTCAT CTCCCTGGCA GGCCCGGGGA GGCAAGTCAG GAGCTGCTTT CTATGCCACC 
GAAGATGATA GATTCATTCT GAAGCAAATG CCTCGTTTGG AAGTCCAGTC TTTCCTTGAC 
TTTGCACCAC ACTACTTCAA TTATATCACA AATGCTGTTC AACAAAAGAG GCCCACCGCC 
TTGGCTAAAA TTCTTGGAGT TTACAGAATT GGCTATAAGA ACTCTCAGAA CAACACTGAG 
AAGAAGTTAG ATCTCCTTGT TATGGAAAAT CTTTTCTATG GGAGAAAGAT GGCACAGGTT 
TTTGATTTGA AGGGTTCACT TAGGAATCGA AATGTAAAAA CTGACACTGG GAAAGAGAGC 
TGTGATGTGG TTCTGTTGGA TGAAAACCTC CTAAAGATGG TTCGAGACAA CCCTCTCTAT 
ATTCGTTCCC ATTCCAAATC TGTGCTGAGA ACCTCCATCC ACAGCGACGC CCATTTCCTT 
TCCAGCCACC TCATTATAGA CTATTCTCTG CTGGTTGGGC GAGATGACAC TAGCAATGAG 
CTTGTGGTTG GCATCATAGA TTACATTCGA ACATTTACAT GGGACAAAAA ACTTGAGATG 
GTTGTGAAGT CAACAGGAAT TTTAGGAGGA CAAGGTAAAA TGCCAACTGT GGTCTCTCCA 
GAGTTGTATA GGACTAGATT TTGTGAAGCA ATGGACAAGT ATTTCTTGAT GGTGCCAGAC 
CACTGGACAG GGTTGGATCT GAATTGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_029331924.1
CDS	164..6493
Translation	MATDDKSSPTLDSANDLPRSPASPSHLTHFKPLTPDQDEPPFKSAYSSFVNLFRFNKERGEGGQGEQQSPSSSWASPQIPSRTQSVRSPVPYKKQLNEELHRRSSVLAENTLPHPQESTDSRRKAEPACGGHDPRTAVQLRSLSTVLKRLKEIMEGKSQDSDLKQYWMPDSQCKECYDCSEKFTTFRRRHHCRLCGQIFCSRCCNQEIPGKFMGYTGDLRACTYCRKIALSYAHSTDSNSIGEDLNALSDSTCSVSILDPSEPRTPVGSRKASRNIFLEDDLAWQSLIHPDSSNSALSTRLVSVQEDAGKSPARNRSASITNLSLDRSGSPMVPSYETSVSPQANRNYIRTETTEDERKILLDSAQLKDLWKKICHHTSGMEFQDHRYWLRTHPNCIVGKELVNWLIRNGHIATRAQAIAIGQAMVDGRWLDCVSHHDQLFRDEYALYRPLQSTEFSETPSPDSDSVNSVEGHSEPSWFKDIKFDDSDTEQIAEEGDDNLANSASPSKRTSVSSFQSTVDSDSAASISLNVELDNVNFHIKKPSKYPHVPPHPADQKEYLVSDTGGQQLSISDAFIKESLFNRRVEEKSKELPFTPLGWHHNNLELLREENEEKQAMERLLSANHNHMMALLQQLLQNESLSSSWRDIIVSLVCQVVQTVRPDVKHQDDDMDIRQFVHIKKIPGGKKFDSVVVNGFVCTKNIAHKKMNSCIKNPKILLLKCSIEYLYREETKFTCIDPIVLQEREFLKNYVQRIVDVRPTLVLVEKTVSRIAQDMLLEHGITLVINVKSQVLERISRMTQGDLVVSMDQLLTKPHLGTCHKFYMQIFQLPNEQTKTLMFFEGCPQHLGCTIKLRGGSDYELARVKEILIFMICVAYHSQLEISFLMDEFAMPPTLMQSPSFHLLTEGRGEEGASQEQVSGSSLPQDPECPREALSSDDSTLLESRTVLEKGELDNKSIPQAVASLKHQDYTTPTCPAGIPCALFALVPESLLPLHMDQQDAVGNEQPETSQQTDEQQDPKSQMKAFRDPLQDDTGMYVTEEVTSSEDQRKTYALTFKQELKDVILCISPVITFREPFLLTEKGMRCSTRDYFPEQIYWSPLLNKEVMEMESRRKKQLLRDLSGLQGMNGSVQAKSIQVLPSHELVSTRIAEHLGDSQTLGRMLADYRARGGRIQSKHLDPFVHSKDASCTSGGKPGNKTESDEERGLIPSDVIWPAKVDCLNPANHQRLCVLFSSSSAQSSNAPSACVSPWIVTMEFYGKNDLTLGIFLERYCFRSSYQCPSMFCDTPMVHHIRRFVHGQGCVQIILKELDSPVPGYQHTILTYSWCRICKQVTPVVALSNESWSMSFAKYLELRFYGHQYTRRANAEPCGHSIHHDYHQYFSYNQMVASFSYSPIRLLEVCVPLPKIFIKRQAPLKVSLLQDLKDFFQKVSQVYLAVDERLASLKTDTFSKTREEKMEDIFAQKEMEEGEFKNWTEKMQARLMSSSVDTPQQLQSIFESLIAKKQSLCEVLQAWNSRLQDLFQQEKGRKRPSVPPSPGRLRQGEESKINAMDTSPRNISPGLHNGEKEDRFLTTLSSQSSTSSTHLQLPTPPEALAEQVVGGPTDLDSASGSEDVFDGHLLGSTDSQVKEKSTMKAIFANLLPGNSYNPIPFPFDPDKHYLMYEHERVPIAVCEKEPSSIIAFALSCKEYRNALEELSKATLWNSAEEGLPANSALDNRPKSSSPIRVPEISGGQTNRTGEAEPQPTKKASGMLSFFRGTAGKSPDLSSQKRETLRGADSAYYQVGQAGKEGLESQGLEPQDEVDGGDTQKKQLTNPHVELQFSDANAKFYCRLYYAGEFHKMREVILGSSEEDFIRSLSHSSPWQARGGKSGAAFYATEDDRFILKQMPRLEVQSFLDFAPHYFNYITNAVQQKRPTALAKILGVYRIGYKNSQNNTEKKLDLLVMENLFYGRKMAQVFDLKGSLRNRNVKTDTGKESCDVVLLDENLLKMVRDNPLYIRSHSKSVLRTSIHSDAHFLSSHLIIDYSLLVGRDDTSNELVVGIIDYIRTFTWDKKLEMVVKSTGILGGQGKMPTVVSPELYRTRFCEAMDKYFLMVPDHWTGLDLNC

Target ORF information:

RefSeq Version	XM_029476064.1
Organism	Mus caroli(Ryukyu mouse)
Definition	Mus caroli phosphoinositide kinase, FYVE-type zinc finger containing (Pikfyve), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_029476064.1

ORF Insert Sequence:

ATGGCCACAG ATGACAAGAG TTCCCCGACA CTGGACTCTG CTAATGATTT GCCTCGCTCT 
CCCGCCAGTC CTTCTCACCT CACTCACTTT AAACCCTTGA CTCCTGACCA GGATGAGCCC 
CCCTTCAAGT CAGCATATAG TTCTTTTGTA AACCTTTTTC GCTTTAACAA AGAGCGGGGA 
GAAGGGGGCC AAGGAGAGCA GCAGTCTCCG AGTTCAAGTT GGGCCAGCCC TCAGATCCCT 
TCAAGAACAC AGTCTGTGAG GTCGCCTGTA CCTTATAAAA AACAGCTTAA TGAGGAGCTC 
CACCGGCGCT CTTCAGTGTT AGCAGAGAAC ACTTTGCCAC ATCCTCAGGA GAGCACAGAC 
TCCAGAAGGA AAGCAGAACC AGCCTGTGGA GGTCATGACC CACGTACAGC TGTTCAGCTT 
CGAAGCCTCA GCACAGTATT GAAACGCCTC AAAGAAATCA TGGAAGGAAA AAGCCAGGAC 
AGTGACCTGA AGCAATATTG GATGCCAGAT AGCCAGTGTA AAGAGTGCTA TGACTGCAGT 
GAAAAGTTTA CAACATTTAG GCGCAGACAC CATTGCAGAC TGTGTGGGCA GATTTTCTGC 
AGTCGTTGTT GTAATCAAGA AATCCCTGGA AAATTTATGG GCTATACAGG AGACCTCCGA 
GCATGCACCT ACTGTAGGAA AATAGCCTTA AGTTATGCTC ATTCCACAGA CAGTAATTCC 
ATTGGGGAAG ACTTGAATGC TCTTTCAGAT TCAACTTGCT CTGTGTCTAT ACTTGATCCA 
AGCGAACCTC GGACACCTGT TGGGAGTAGA AAAGCCAGTC GTAACATATT CTTAGAGGAT 
GATTTAGCCT GGCAAAGTTT GATTCATCCG GATTCCTCAA ATAGTGCTCT TTCAACAAGA 
CTCGTATCTG TTCAAGAGGA TGCTGGGAAG TCTCCTGCTC GGAACAGATC AGCCAGCATT 
ACTAATCTGT CACTGGATCG GTCTGGTTCT CCTATGGTTC CTTCATATGA GACATCTGTC 
AGTCCCCAGG CTAACCGAAA CTACATTAGG ACCGAGACGA CTGAGGATGA ACGCAAAATT 
CTTCTGGACA GTGCTCAGTT AAAGGATCTG TGGAAGAAAA TCTGCCATCA CACCAGTGGG 
ATGGAATTTC AAGATCACCG TTACTGGTTG AGAACACATC CCAACTGCAT TGTAGGAAAG 
GAATTAGTCA ACTGGCTAAT CAGAAATGGA CACATCGCTA CAAGGGCACA AGCTATAGCA 
ATTGGACAAG CAATGGTTGA TGGACGTTGG TTGGATTGTG TTAGTCATCA TGACCAGCTT 
TTCAGGGACG AATATGCGTT GTATAGACCA CTTCAGAGTA CAGAATTTTC TGAGACACCT 
TCTCCAGACA GTGACTCCGT GAACTCTGTG GAAGGACACT CCGAGCCATC CTGGTTTAAA 
GACATAAAAT TTGATGACAG TGACACAGAA CAGATTGCTG AAGAAGGTGA CGATAATTTG 
GCTAATTCTG CCAGTCCTAG CAAGCGCACC TCAGTCAGCA GTTTCCAGTC CACAGTGGAC 
AGTGACTCAG CCGCTTCTAT CAGCCTGAAC GTGGAGCTGG ACAACGTGAA CTTCCATATC 
AAGAAGCCCT CCAAGTACCC ACATGTGCCC CCTCACCCTG CTGACCAAAA AGAGTATTTG 
GTTTCTGACA CTGGAGGACA GCAGCTCTCA ATAAGTGATG CCTTCATCAA AGAGTCCTTA 
TTTAATCGAC GAGTAGAGGA AAAATCCAAA GAGCTGCCTT TTACCCCTTT GGGCTGGCAT 
CATAACAACC TGGAACTCTT GCGAGAGGAG AATGAGGAGA AGCAAGCCAT GGAAAGGCTG 
CTTTCAGCTA ATCATAACCA CATGATGGCC CTACTCCAGC AATTGCTTCA AAACGAGTCA 
TTGTCATCGT CTTGGAGGGA CATCATTGTG TCACTAGTCT GCCAGGTTGT TCAGACAGTC 
CGACCTGACG TCAAGCACCA GGATGATGAC ATGGATATCC GTCAGTTTGT CCATATCAAG 
AAGATCCCAG GTGGAAAGAA ATTTGATTCT GTGGTTGTCA ATGGCTTTGT TTGTACCAAG 
AACATTGCAC ATAAAAAGAT GAATTCCTGT ATTAAAAACC CCAAAATCCT TCTGTTGAAG 
TGTTCTATTG AGTATCTCTA TAGAGAAGAA ACTAAGTTTA CCTGCATTGA TCCTATTGTG 
CTTCAGGAAA GGGAATTCTT GAAGAATTAT GTTCAACGAA TAGTTGATGT TCGACCCACA 
TTGGTTCTTG TTGAGAAAAC CGTGTCTCGG ATTGCTCAGG ACATGTTACT GGAACATGGC 
ATTACTCTGG TCATTAATGT AAAGTCACAA GTTTTAGAAA GAATCAGTCG AATGACCCAA 
GGTGATTTAG TGGTGTCCAT GGACCAGCTG CTCACCAAAC CCCACTTGGG CACTTGCCAC 
AAATTTTATA TGCAGATATT TCAGCTGCCT AATGAACAAA CCAAAACACT GATGTTTTTT 
GAAGGCTGTC CACAGCACCT AGGCTGCACA ATCAAGCTCA GAGGAGGCTC TGATTATGAG 
CTGGCTCGAG TTAAGGAGAT CCTAATATTT ATGATCTGTG TAGCTTATCA TTCTCAGCTA 
GAAATCTCTT TTCTCATGGA TGAGTTCGCT ATGCCTCCAA CGTTAATGCA AAGCCCTTCG 
TTCCATCTTC TGACGGAGGG ACGGGGTGAA GAGGGAGCCT CTCAGGAGCA GGTCAGTGGC 
AGCTCCCTTC CTCAGGATCC TGAGTGCCCT CGTGAGGCCC TGTCTTCTGA CGATAGCACT 
TTGTTGGAAT CAAGGACTGT GCTAGAGAAG GGTGAACTAG ACAATAAAAG TATTCCACAA 
GCTGTTGCCT CTTTGAAGCA TCAAGATTAT ACCACCCCCA CTTGCCCAGC AGGTATCCCC 
TGTGCTCTCT TTGCATTGGT ACCAGAGTCA TTGTTGCCTC TCCATATGGA TCAACAGGAT 
GCTGTAGGAA ATGAACAACC AGAGACATCA CAACAAACAG ATGAGCAACA GGATCCCAAA 
AGCCAGATGA AAGCTTTTAG AGACCCTCTA CAGGATGACA CCGGAATGTA TGTTACTGAG 
GAAGTCACCT CCTCTGAAGA TCAACGAAAG ACTTATGCCT TGACATTTAA ACAGGAATTA 
AAAGACGTAA TCCTCTGTAT CTCTCCAGTT ATTACATTCC GTGAACCTTT CCTTTTAACT 
GAAAAGGGGA TGAGATGCTC AACTCGAGAT TATTTTCCAG AGCAGATTTA CTGGTCTCCT 
CTTCTCAACA AAGAGGTGAT GGAAATGGAG AGCAGGAGGA AGAAACAGCT GCTCAGGGAT 
CTCTCTGGAC TTCAGGGCAT GAATGGCAGT GTTCAGGCCA AGTCTATTCA AGTCTTACCC 
TCACATGAAC TAGTGAGCAC CAGGATTGCT GAACATCTGG GTGACAGCCA GACCTTGGGT 
AGAATGCTAG CTGATTATCG AGCTAGAGGA GGAAGAATTC AGTCAAAACA TTTGGACCCT 
TTTGTCCATT CAAAAGATGC ATCATGTACT TCAGGTGGAA AACCAGGAAA CAAAACTGAG 
AGTGATGAAG AGAGGGGATT GATCCCAAGT GATGTAATAT GGCCAGCAAA GGTGGACTGT 
CTGAATCCTG CTAACCACCA GAGGCTCTGT GTGCTCTTCA GCAGCTCTTC TGCCCAGTCC 
AGCAATGCTC CCAGTGCTTG TGTCAGTCCT TGGATTGTAA CAATGGAGTT TTATGGAAAG 
AATGACCTTA CACTGGGAAT ATTTTTAGAA AGATACTGTT TCAGGTCTTC TTACCAGTGT 
CCGAGCATGT TCTGTGACAC CCCCATGGTT CATCACATTC GACGCTTTGT TCATGGCCAA 
GGCTGTGTAC AGATAATTCT GAAGGAGTTG GATTCTCCAG TGCCTGGATA TCAACATACA 
ATTCTCACAT ACTCCTGGTG CAGAATCTGC AAACAAGTAA CACCAGTTGT TGCTCTTTCA 
AATGAATCCT GGTCTATGTC ATTTGCAAAG TACCTTGAAC TTCGATTTTA TGGCCACCAG 
TACACACGCA GAGCCAACGC TGAGCCCTGC GGTCACTCTA TCCACCATGA TTATCACCAG 
TATTTCTCTT ATAACCAGAT GGTGGCATCT TTCAGTTACT CTCCTATTCG GCTTCTTGAA 
GTATGTGTTC CACTACCAAA AATATTCATT AAGCGTCAAG CCCCACTGAA GGTATCTCTT 
CTTCAGGACC TCAAGGACTT TTTTCAGAAG GTTTCACAGG TGTACCTAGC TGTTGATGAG 
AGACTTGCAT CCTTGAAAAC GGATACATTT AGCAAAACCA GAGAGGAAAA GATGGAAGAT 
ATCTTTGCAC AAAAGGAGAT GGAAGAGGGT GAGTTTAAGA ACTGGACAGA GAAGATGCAA 
GCAAGGCTCA TGTCTTCCTC TGTGGATACC CCTCAGCAAC TGCAGTCCAT TTTTGAGTCA 
CTGATTGCCA AGAAGCAAAG CCTCTGTGAG GTGCTCCAGG CGTGGAACAG CAGGTTGCAG 
GACCTCTTCC AGCAGGAAAA AGGTAGAAAG AGGCCTTCAG TTCCTCCCAG TCCTGGGAGA 
CTGAGACAAG GTGAAGAAAG CAAGATAAAT GCAATGGACA CATCTCCAAG GAATATTTCT 
CCAGGACTTC ACAATGGAGA AAAAGAGGAT CGCTTCTTGA CAACCCTGTC CAGCCAGAGC 
TCTACGAGCT CCACCCACCT CCAGCTGCCC ACTCCTCCCG AGGCCCTGGC CGAGCAGGTA 
GTGGGAGGGC CGACTGATCT GGATTCAGCC AGTGGTTCTG AAGATGTATT TGATGGTCAT 
TTGCTGGGAT CCACAGACAG CCAGGTGAAG GAAAAGTCAA CCATGAAAGC CATCTTTGCT 
AATTTGCTTC CAGGAAACAG CTACAATCCC ATTCCATTTC CTTTTGATCC AGATAAACAC 
TACTTAATGT ATGAACATGA ACGGGTGCCC ATTGCTGTCT GTGAGAAAGA GCCCAGCTCC 
ATCATTGCTT TTGCACTCAG TTGTAAAGAA TACCGAAATG CCTTAGAGGA ATTGTCCAAA 
GCGACTCTGT GGAACAGTGC TGAAGAAGGG CTTCCAGCCA ATAGTGCTTT AGATAACAGA 
CCTAAGAGTA GCAGCCCTAT TAGAGTACCT GAAATCAGTG GAGGACAGAC AAACCGCACA 
GGAGAAGCAG AACCTCAGCC AACCAAAAAA GCTTCAGGCA TGTTGTCCTT CTTCAGAGGA 
ACAGCAGGGA AGAGTCCTGA CCTGTCTTCT CAGAAGAGGG AGACCTTACG TGGGGCAGAC 
AGTGCTTACT ACCAGGTTGG GCAGGCTGGC AAGGAGGGGT TGGAGAGTCA AGGCCTGGAG 
CCTCAAGATG AAGTAGACGG AGGAGATACA CAGAAGAAAC AACTCACAAA TCCTCATGTG 
GAACTTCAAT TTTCTGATGC TAATGCCAAG TTTTACTGTC GGCTGTACTA CGCGGGAGAG 
TTCCATAAGA TGCGTGAAGT GATTCTGGGC AGCAGCGAGG AGGATTTCAT TCGCTCCCTT 
TCTCACTCAT CTCCCTGGCA GGCCCGGGGA GGCAAGTCAG GAGCTGCTTT CTATGCCACC 
GAAGATGATA GATTCATTCT GAAGCAAATG CCTCGTTTGG AAGTCCAGTC TTTCCTTGAC 
TTTGCACCAC ACTACTTCAA TTATATCACA AATGCTGTTC AACAAAAGAG GCCCACCGCC 
TTGGCTAAAA TTCTTGGAGT TTACAGAATT GGCTATAAGA ACTCTCAGAA CAACACTGAG 
AAGAAGTTAG ATCTCCTTGT TATGGAAAAT CTTTTCTATG GGAGAAAGAT GGCACAGGTT 
TTTGATTTGA AGGGTTCACT TAGGAATCGA AATGTAAAAA CTGACACTGG GAAAGAGAGC 
TGTGATGTGG TTCTGTTGGA TGAAAACCTC CTAAAGATGG TTCGAGACAA CCCTCTCTAT 
ATTCGTTCCC ATTCCAAATC TGTGCTGAGA ACCTCCATCC ACAGCGACGC CCATTTCCTT 
TCCAGCCACC TCATTATAGA CTATTCTCTG CTGGTTGGGC GAGATGACAC TAGCAATGAG 
CTTGTGGTTG GCATCATAGA TTACATTCGA ACATTTACAT GGGACAAAAA ACTTGAGATG 
GTTGTGAAGT CAACAGGAAT TTTAGGAGGA CAAGGTAAAA TGCCAACTGT GGTCTCTCCA 
GAGTTGTATA GGACTAGATT TTGTGAAGCA ATGGACAAGT ATTTCTTGAT GGTGCCAGAC 
CACTGGACAG GGTTGGATCT GAATTGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Pikfyve ( XM_029476067.1 ) cDNA ORF clone, Mus caroli(Ryukyu mouse) -> XP_029331927.1 Mus caroli phosphoinositide kinase, FYVE-type zinc finger containing (Pikfyve), transcript variant X2, mRNA.

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CloneID	OMv66195
Clone ID Related Accession (Same CDS sequence)	XM_029476064.1 , XM_029476067.1
Accession Version	XM_029476067.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6330bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-06-09
Organism	Mus caroli(Ryukyu mouse)
Product	1-phosphatidylinositol 3-phosphate 5-kinase isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_034570.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus caroli Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCACAG ATGACAAGAG TTCCCCGACA CTGGACTCTG CTAATGATTT GCCTCGCTCT 
CCCGCCAGTC CTTCTCACCT CACTCACTTT AAACCCTTGA CTCCTGACCA GGATGAGCCC 
CCCTTCAAGT CAGCATATAG TTCTTTTGTA AACCTTTTTC GCTTTAACAA AGAGCGGGGA 
GAAGGGGGCC AAGGAGAGCA GCAGTCTCCG AGTTCAAGTT GGGCCAGCCC TCAGATCCCT 
TCAAGAACAC AGTCTGTGAG GTCGCCTGTA CCTTATAAAA AACAGCTTAA TGAGGAGCTC 
CACCGGCGCT CTTCAGTGTT AGCAGAGAAC ACTTTGCCAC ATCCTCAGGA GAGCACAGAC 
TCCAGAAGGA AAGCAGAACC AGCCTGTGGA GGTCATGACC CACGTACAGC TGTTCAGCTT 
CGAAGCCTCA GCACAGTATT GAAACGCCTC AAAGAAATCA TGGAAGGAAA AAGCCAGGAC 
AGTGACCTGA AGCAATATTG GATGCCAGAT AGCCAGTGTA AAGAGTGCTA TGACTGCAGT 
GAAAAGTTTA CAACATTTAG GCGCAGACAC CATTGCAGAC TGTGTGGGCA GATTTTCTGC 
AGTCGTTGTT GTAATCAAGA AATCCCTGGA AAATTTATGG GCTATACAGG AGACCTCCGA 
GCATGCACCT ACTGTAGGAA AATAGCCTTA AGTTATGCTC ATTCCACAGA CAGTAATTCC 
ATTGGGGAAG ACTTGAATGC TCTTTCAGAT TCAACTTGCT CTGTGTCTAT ACTTGATCCA 
AGCGAACCTC GGACACCTGT TGGGAGTAGA AAAGCCAGTC GTAACATATT CTTAGAGGAT 
GATTTAGCCT GGCAAAGTTT GATTCATCCG GATTCCTCAA ATAGTGCTCT TTCAACAAGA 
CTCGTATCTG TTCAAGAGGA TGCTGGGAAG TCTCCTGCTC GGAACAGATC AGCCAGCATT 
ACTAATCTGT CACTGGATCG GTCTGGTTCT CCTATGGTTC CTTCATATGA GACATCTGTC 
AGTCCCCAGG CTAACCGAAA CTACATTAGG ACCGAGACGA CTGAGGATGA ACGCAAAATT 
CTTCTGGACA GTGCTCAGTT AAAGGATCTG TGGAAGAAAA TCTGCCATCA CACCAGTGGG 
ATGGAATTTC AAGATCACCG TTACTGGTTG AGAACACATC CCAACTGCAT TGTAGGAAAG 
GAATTAGTCA ACTGGCTAAT CAGAAATGGA CACATCGCTA CAAGGGCACA AGCTATAGCA 
ATTGGACAAG CAATGGTTGA TGGACGTTGG TTGGATTGTG TTAGTCATCA TGACCAGCTT 
TTCAGGGACG AATATGCGTT GTATAGACCA CTTCAGAGTA CAGAATTTTC TGAGACACCT 
TCTCCAGACA GTGACTCCGT GAACTCTGTG GAAGGACACT CCGAGCCATC CTGGTTTAAA 
GACATAAAAT TTGATGACAG TGACACAGAA CAGATTGCTG AAGAAGGTGA CGATAATTTG 
GCTAATTCTG CCAGTCCTAG CAAGCGCACC TCAGTCAGCA GTTTCCAGTC CACAGTGGAC 
AGTGACTCAG CCGCTTCTAT CAGCCTGAAC GTGGAGCTGG ACAACGTGAA CTTCCATATC 
AAGAAGCCCT CCAAGTACCC ACATGTGCCC CCTCACCCTG CTGACCAAAA AGAGTATTTG 
GTTTCTGACA CTGGAGGACA GCAGCTCTCA ATAAGTGATG CCTTCATCAA AGAGTCCTTA 
TTTAATCGAC GAGTAGAGGA AAAATCCAAA GAGCTGCCTT TTACCCCTTT GGGCTGGCAT 
CATAACAACC TGGAACTCTT GCGAGAGGAG AATGAGGAGA AGCAAGCCAT GGAAAGGCTG 
CTTTCAGCTA ATCATAACCA CATGATGGCC CTACTCCAGC AATTGCTTCA AAACGAGTCA 
TTGTCATCGT CTTGGAGGGA CATCATTGTG TCACTAGTCT GCCAGGTTGT TCAGACAGTC 
CGACCTGACG TCAAGCACCA GGATGATGAC ATGGATATCC GTCAGTTTGT CCATATCAAG 
AAGATCCCAG GTGGAAAGAA ATTTGATTCT GTGGTTGTCA ATGGCTTTGT TTGTACCAAG 
AACATTGCAC ATAAAAAGAT GAATTCCTGT ATTAAAAACC CCAAAATCCT TCTGTTGAAG 
TGTTCTATTG AGTATCTCTA TAGAGAAGAA ACTAAGTTTA CCTGCATTGA TCCTATTGTG 
CTTCAGGAAA GGGAATTCTT GAAGAATTAT GTTCAACGAA TAGTTGATGT TCGACCCACA 
TTGGTTCTTG TTGAGAAAAC CGTGTCTCGG ATTGCTCAGG ACATGTTACT GGAACATGGC 
ATTACTCTGG TCATTAATGT AAAGTCACAA GTTTTAGAAA GAATCAGTCG AATGACCCAA 
GGTGATTTAG TGGTGTCCAT GGACCAGCTG CTCACCAAAC CCCACTTGGG CACTTGCCAC 
AAATTTTATA TGCAGATATT TCAGCTGCCT AATGAACAAA CCAAAACACT GATGTTTTTT 
GAAGGCTGTC CACAGCACCT AGGCTGCACA ATCAAGCTCA GAGGAGGCTC TGATTATGAG 
CTGGCTCGAG TTAAGGAGAT CCTAATATTT ATGATCTGTG TAGCTTATCA TTCTCAGCTA 
GAAATCTCTT TTCTCATGGA TGAGTTCGCT ATGCCTCCAA CGTTAATGCA AAGCCCTTCG 
TTCCATCTTC TGACGGAGGG ACGGGGTGAA GAGGGAGCCT CTCAGGAGCA GGTCAGTGGC 
AGCTCCCTTC CTCAGGATCC TGAGTGCCCT CGTGAGGCCC TGTCTTCTGA CGATAGCACT 
TTGTTGGAAT CAAGGACTGT GCTAGAGAAG GGTGAACTAG ACAATAAAAG TATTCCACAA 
GCTGTTGCCT CTTTGAAGCA TCAAGATTAT ACCACCCCCA CTTGCCCAGC AGGTATCCCC 
TGTGCTCTCT TTGCATTGGT ACCAGAGTCA TTGTTGCCTC TCCATATGGA TCAACAGGAT 
GCTGTAGGAA ATGAACAACC AGAGACATCA CAACAAACAG ATGAGCAACA GGATCCCAAA 
AGCCAGATGA AAGCTTTTAG AGACCCTCTA CAGGATGACA CCGGAATGTA TGTTACTGAG 
GAAGTCACCT CCTCTGAAGA TCAACGAAAG ACTTATGCCT TGACATTTAA ACAGGAATTA 
AAAGACGTAA TCCTCTGTAT CTCTCCAGTT ATTACATTCC GTGAACCTTT CCTTTTAACT 
GAAAAGGGGA TGAGATGCTC AACTCGAGAT TATTTTCCAG AGCAGATTTA CTGGTCTCCT 
CTTCTCAACA AAGAGGTGAT GGAAATGGAG AGCAGGAGGA AGAAACAGCT GCTCAGGGAT 
CTCTCTGGAC TTCAGGGCAT GAATGGCAGT GTTCAGGCCA AGTCTATTCA AGTCTTACCC 
TCACATGAAC TAGTGAGCAC CAGGATTGCT GAACATCTGG GTGACAGCCA GACCTTGGGT 
AGAATGCTAG CTGATTATCG AGCTAGAGGA GGAAGAATTC AGTCAAAACA TTTGGACCCT 
TTTGTCCATT CAAAAGATGC ATCATGTACT TCAGGTGGAA AACCAGGAAA CAAAACTGAG 
AGTGATGAAG AGAGGGGATT GATCCCAAGT GATGTAATAT GGCCAGCAAA GGTGGACTGT 
CTGAATCCTG CTAACCACCA GAGGCTCTGT GTGCTCTTCA GCAGCTCTTC TGCCCAGTCC 
AGCAATGCTC CCAGTGCTTG TGTCAGTCCT TGGATTGTAA CAATGGAGTT TTATGGAAAG 
AATGACCTTA CACTGGGAAT ATTTTTAGAA AGATACTGTT TCAGGTCTTC TTACCAGTGT 
CCGAGCATGT TCTGTGACAC CCCCATGGTT CATCACATTC GACGCTTTGT TCATGGCCAA 
GGCTGTGTAC AGATAATTCT GAAGGAGTTG GATTCTCCAG TGCCTGGATA TCAACATACA 
ATTCTCACAT ACTCCTGGTG CAGAATCTGC AAACAAGTAA CACCAGTTGT TGCTCTTTCA 
AATGAATCCT GGTCTATGTC ATTTGCAAAG TACCTTGAAC TTCGATTTTA TGGCCACCAG 
TACACACGCA GAGCCAACGC TGAGCCCTGC GGTCACTCTA TCCACCATGA TTATCACCAG 
TATTTCTCTT ATAACCAGAT GGTGGCATCT TTCAGTTACT CTCCTATTCG GCTTCTTGAA 
GTATGTGTTC CACTACCAAA AATATTCATT AAGCGTCAAG CCCCACTGAA GGTATCTCTT 
CTTCAGGACC TCAAGGACTT TTTTCAGAAG GTTTCACAGG TGTACCTAGC TGTTGATGAG 
AGACTTGCAT CCTTGAAAAC GGATACATTT AGCAAAACCA GAGAGGAAAA GATGGAAGAT 
ATCTTTGCAC AAAAGGAGAT GGAAGAGGGT GAGTTTAAGA ACTGGACAGA GAAGATGCAA 
GCAAGGCTCA TGTCTTCCTC TGTGGATACC CCTCAGCAAC TGCAGTCCAT TTTTGAGTCA 
CTGATTGCCA AGAAGCAAAG CCTCTGTGAG GTGCTCCAGG CGTGGAACAG CAGGTTGCAG 
GACCTCTTCC AGCAGGAAAA AGGTAGAAAG AGGCCTTCAG TTCCTCCCAG TCCTGGGAGA 
CTGAGACAAG GTGAAGAAAG CAAGATAAAT GCAATGGACA CATCTCCAAG GAATATTTCT 
CCAGGACTTC ACAATGGAGA AAAAGAGGAT CGCTTCTTGA CAACCCTGTC CAGCCAGAGC 
TCTACGAGCT CCACCCACCT CCAGCTGCCC ACTCCTCCCG AGGCCCTGGC CGAGCAGGTA 
GTGGGAGGGC CGACTGATCT GGATTCAGCC AGTGGTTCTG AAGATGTATT TGATGGTCAT 
TTGCTGGGAT CCACAGACAG CCAGGTGAAG GAAAAGTCAA CCATGAAAGC CATCTTTGCT 
AATTTGCTTC CAGGAAACAG CTACAATCCC ATTCCATTTC CTTTTGATCC AGATAAACAC 
TACTTAATGT ATGAACATGA ACGGGTGCCC ATTGCTGTCT GTGAGAAAGA GCCCAGCTCC 
ATCATTGCTT TTGCACTCAG TTGTAAAGAA TACCGAAATG CCTTAGAGGA ATTGTCCAAA 
GCGACTCTGT GGAACAGTGC TGAAGAAGGG CTTCCAGCCA ATAGTGCTTT AGATAACAGA 
CCTAAGAGTA GCAGCCCTAT TAGAGTACCT GAAATCAGTG GAGGACAGAC AAACCGCACA 
GGAGAAGCAG AACCTCAGCC AACCAAAAAA GCTTCAGGCA TGTTGTCCTT CTTCAGAGGA 
ACAGCAGGGA AGAGTCCTGA CCTGTCTTCT CAGAAGAGGG AGACCTTACG TGGGGCAGAC 
AGTGCTTACT ACCAGGTTGG GCAGGCTGGC AAGGAGGGGT TGGAGAGTCA AGGCCTGGAG 
CCTCAAGATG AAGTAGACGG AGGAGATACA CAGAAGAAAC AACTCACAAA TCCTCATGTG 
GAACTTCAAT TTTCTGATGC TAATGCCAAG TTTTACTGTC GGCTGTACTA CGCGGGAGAG 
TTCCATAAGA TGCGTGAAGT GATTCTGGGC AGCAGCGAGG AGGATTTCAT TCGCTCCCTT 
TCTCACTCAT CTCCCTGGCA GGCCCGGGGA GGCAAGTCAG GAGCTGCTTT CTATGCCACC 
GAAGATGATA GATTCATTCT GAAGCAAATG CCTCGTTTGG AAGTCCAGTC TTTCCTTGAC 
TTTGCACCAC ACTACTTCAA TTATATCACA AATGCTGTTC AACAAAAGAG GCCCACCGCC 
TTGGCTAAAA TTCTTGGAGT TTACAGAATT GGCTATAAGA ACTCTCAGAA CAACACTGAG 
AAGAAGTTAG ATCTCCTTGT TATGGAAAAT CTTTTCTATG GGAGAAAGAT GGCACAGGTT 
TTTGATTTGA AGGGTTCACT TAGGAATCGA AATGTAAAAA CTGACACTGG GAAAGAGAGC 
TGTGATGTGG TTCTGTTGGA TGAAAACCTC CTAAAGATGG TTCGAGACAA CCCTCTCTAT 
ATTCGTTCCC ATTCCAAATC TGTGCTGAGA ACCTCCATCC ACAGCGACGC CCATTTCCTT 
TCCAGCCACC TCATTATAGA CTATTCTCTG CTGGTTGGGC GAGATGACAC TAGCAATGAG 
CTTGTGGTTG GCATCATAGA TTACATTCGA ACATTTACAT GGGACAAAAA ACTTGAGATG 
GTTGTGAAGT CAACAGGAAT TTTAGGAGGA CAAGGTAAAA TGCCAACTGT GGTCTCTCCA 
GAGTTGTATA GGACTAGATT TTGTGAAGCA ATGGACAAGT ATTTCTTGAT GGTGCCAGAC 
CACTGGACAG GGTTGGATCT GAATTGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_029331927.1
CDS	100..6429
Translation	MATDDKSSPTLDSANDLPRSPASPSHLTHFKPLTPDQDEPPFKSAYSSFVNLFRFNKERGEGGQGEQQSPSSSWASPQIPSRTQSVRSPVPYKKQLNEELHRRSSVLAENTLPHPQESTDSRRKAEPACGGHDPRTAVQLRSLSTVLKRLKEIMEGKSQDSDLKQYWMPDSQCKECYDCSEKFTTFRRRHHCRLCGQIFCSRCCNQEIPGKFMGYTGDLRACTYCRKIALSYAHSTDSNSIGEDLNALSDSTCSVSILDPSEPRTPVGSRKASRNIFLEDDLAWQSLIHPDSSNSALSTRLVSVQEDAGKSPARNRSASITNLSLDRSGSPMVPSYETSVSPQANRNYIRTETTEDERKILLDSAQLKDLWKKICHHTSGMEFQDHRYWLRTHPNCIVGKELVNWLIRNGHIATRAQAIAIGQAMVDGRWLDCVSHHDQLFRDEYALYRPLQSTEFSETPSPDSDSVNSVEGHSEPSWFKDIKFDDSDTEQIAEEGDDNLANSASPSKRTSVSSFQSTVDSDSAASISLNVELDNVNFHIKKPSKYPHVPPHPADQKEYLVSDTGGQQLSISDAFIKESLFNRRVEEKSKELPFTPLGWHHNNLELLREENEEKQAMERLLSANHNHMMALLQQLLQNESLSSSWRDIIVSLVCQVVQTVRPDVKHQDDDMDIRQFVHIKKIPGGKKFDSVVVNGFVCTKNIAHKKMNSCIKNPKILLLKCSIEYLYREETKFTCIDPIVLQEREFLKNYVQRIVDVRPTLVLVEKTVSRIAQDMLLEHGITLVINVKSQVLERISRMTQGDLVVSMDQLLTKPHLGTCHKFYMQIFQLPNEQTKTLMFFEGCPQHLGCTIKLRGGSDYELARVKEILIFMICVAYHSQLEISFLMDEFAMPPTLMQSPSFHLLTEGRGEEGASQEQVSGSSLPQDPECPREALSSDDSTLLESRTVLEKGELDNKSIPQAVASLKHQDYTTPTCPAGIPCALFALVPESLLPLHMDQQDAVGNEQPETSQQTDEQQDPKSQMKAFRDPLQDDTGMYVTEEVTSSEDQRKTYALTFKQELKDVILCISPVITFREPFLLTEKGMRCSTRDYFPEQIYWSPLLNKEVMEMESRRKKQLLRDLSGLQGMNGSVQAKSIQVLPSHELVSTRIAEHLGDSQTLGRMLADYRARGGRIQSKHLDPFVHSKDASCTSGGKPGNKTESDEERGLIPSDVIWPAKVDCLNPANHQRLCVLFSSSSAQSSNAPSACVSPWIVTMEFYGKNDLTLGIFLERYCFRSSYQCPSMFCDTPMVHHIRRFVHGQGCVQIILKELDSPVPGYQHTILTYSWCRICKQVTPVVALSNESWSMSFAKYLELRFYGHQYTRRANAEPCGHSIHHDYHQYFSYNQMVASFSYSPIRLLEVCVPLPKIFIKRQAPLKVSLLQDLKDFFQKVSQVYLAVDERLASLKTDTFSKTREEKMEDIFAQKEMEEGEFKNWTEKMQARLMSSSVDTPQQLQSIFESLIAKKQSLCEVLQAWNSRLQDLFQQEKGRKRPSVPPSPGRLRQGEESKINAMDTSPRNISPGLHNGEKEDRFLTTLSSQSSTSSTHLQLPTPPEALAEQVVGGPTDLDSASGSEDVFDGHLLGSTDSQVKEKSTMKAIFANLLPGNSYNPIPFPFDPDKHYLMYEHERVPIAVCEKEPSSIIAFALSCKEYRNALEELSKATLWNSAEEGLPANSALDNRPKSSSPIRVPEISGGQTNRTGEAEPQPTKKASGMLSFFRGTAGKSPDLSSQKRETLRGADSAYYQVGQAGKEGLESQGLEPQDEVDGGDTQKKQLTNPHVELQFSDANAKFYCRLYYAGEFHKMREVILGSSEEDFIRSLSHSSPWQARGGKSGAAFYATEDDRFILKQMPRLEVQSFLDFAPHYFNYITNAVQQKRPTALAKILGVYRIGYKNSQNNTEKKLDLLVMENLFYGRKMAQVFDLKGSLRNRNVKTDTGKESCDVVLLDENLLKMVRDNPLYIRSHSKSVLRTSIHSDAHFLSSHLIIDYSLLVGRDDTSNELVVGIIDYIRTFTWDKKLEMVVKSTGILGGQGKMPTVVSPELYRTRFCEAMDKYFLMVPDHWTGLDLNC

Target ORF information:

RefSeq Version	XM_029476067.1
Organism	Mus caroli(Ryukyu mouse)
Definition	Mus caroli phosphoinositide kinase, FYVE-type zinc finger containing (Pikfyve), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_029476067.1

ORF Insert Sequence:

ATGGCCACAG ATGACAAGAG TTCCCCGACA CTGGACTCTG CTAATGATTT GCCTCGCTCT 
CCCGCCAGTC CTTCTCACCT CACTCACTTT AAACCCTTGA CTCCTGACCA GGATGAGCCC 
CCCTTCAAGT CAGCATATAG TTCTTTTGTA AACCTTTTTC GCTTTAACAA AGAGCGGGGA 
GAAGGGGGCC AAGGAGAGCA GCAGTCTCCG AGTTCAAGTT GGGCCAGCCC TCAGATCCCT 
TCAAGAACAC AGTCTGTGAG GTCGCCTGTA CCTTATAAAA AACAGCTTAA TGAGGAGCTC 
CACCGGCGCT CTTCAGTGTT AGCAGAGAAC ACTTTGCCAC ATCCTCAGGA GAGCACAGAC 
TCCAGAAGGA AAGCAGAACC AGCCTGTGGA GGTCATGACC CACGTACAGC TGTTCAGCTT 
CGAAGCCTCA GCACAGTATT GAAACGCCTC AAAGAAATCA TGGAAGGAAA AAGCCAGGAC 
AGTGACCTGA AGCAATATTG GATGCCAGAT AGCCAGTGTA AAGAGTGCTA TGACTGCAGT 
GAAAAGTTTA CAACATTTAG GCGCAGACAC CATTGCAGAC TGTGTGGGCA GATTTTCTGC 
AGTCGTTGTT GTAATCAAGA AATCCCTGGA AAATTTATGG GCTATACAGG AGACCTCCGA 
GCATGCACCT ACTGTAGGAA AATAGCCTTA AGTTATGCTC ATTCCACAGA CAGTAATTCC 
ATTGGGGAAG ACTTGAATGC TCTTTCAGAT TCAACTTGCT CTGTGTCTAT ACTTGATCCA 
AGCGAACCTC GGACACCTGT TGGGAGTAGA AAAGCCAGTC GTAACATATT CTTAGAGGAT 
GATTTAGCCT GGCAAAGTTT GATTCATCCG GATTCCTCAA ATAGTGCTCT TTCAACAAGA 
CTCGTATCTG TTCAAGAGGA TGCTGGGAAG TCTCCTGCTC GGAACAGATC AGCCAGCATT 
ACTAATCTGT CACTGGATCG GTCTGGTTCT CCTATGGTTC CTTCATATGA GACATCTGTC 
AGTCCCCAGG CTAACCGAAA CTACATTAGG ACCGAGACGA CTGAGGATGA ACGCAAAATT 
CTTCTGGACA GTGCTCAGTT AAAGGATCTG TGGAAGAAAA TCTGCCATCA CACCAGTGGG 
ATGGAATTTC AAGATCACCG TTACTGGTTG AGAACACATC CCAACTGCAT TGTAGGAAAG 
GAATTAGTCA ACTGGCTAAT CAGAAATGGA CACATCGCTA CAAGGGCACA AGCTATAGCA 
ATTGGACAAG CAATGGTTGA TGGACGTTGG TTGGATTGTG TTAGTCATCA TGACCAGCTT 
TTCAGGGACG AATATGCGTT GTATAGACCA CTTCAGAGTA CAGAATTTTC TGAGACACCT 
TCTCCAGACA GTGACTCCGT GAACTCTGTG GAAGGACACT CCGAGCCATC CTGGTTTAAA 
GACATAAAAT TTGATGACAG TGACACAGAA CAGATTGCTG AAGAAGGTGA CGATAATTTG 
GCTAATTCTG CCAGTCCTAG CAAGCGCACC TCAGTCAGCA GTTTCCAGTC CACAGTGGAC 
AGTGACTCAG CCGCTTCTAT CAGCCTGAAC GTGGAGCTGG ACAACGTGAA CTTCCATATC 
AAGAAGCCCT CCAAGTACCC ACATGTGCCC CCTCACCCTG CTGACCAAAA AGAGTATTTG 
GTTTCTGACA CTGGAGGACA GCAGCTCTCA ATAAGTGATG CCTTCATCAA AGAGTCCTTA 
TTTAATCGAC GAGTAGAGGA AAAATCCAAA GAGCTGCCTT TTACCCCTTT GGGCTGGCAT 
CATAACAACC TGGAACTCTT GCGAGAGGAG AATGAGGAGA AGCAAGCCAT GGAAAGGCTG 
CTTTCAGCTA ATCATAACCA CATGATGGCC CTACTCCAGC AATTGCTTCA AAACGAGTCA 
TTGTCATCGT CTTGGAGGGA CATCATTGTG TCACTAGTCT GCCAGGTTGT TCAGACAGTC 
CGACCTGACG TCAAGCACCA GGATGATGAC ATGGATATCC GTCAGTTTGT CCATATCAAG 
AAGATCCCAG GTGGAAAGAA ATTTGATTCT GTGGTTGTCA ATGGCTTTGT TTGTACCAAG 
AACATTGCAC ATAAAAAGAT GAATTCCTGT ATTAAAAACC CCAAAATCCT TCTGTTGAAG 
TGTTCTATTG AGTATCTCTA TAGAGAAGAA ACTAAGTTTA CCTGCATTGA TCCTATTGTG 
CTTCAGGAAA GGGAATTCTT GAAGAATTAT GTTCAACGAA TAGTTGATGT TCGACCCACA 
TTGGTTCTTG TTGAGAAAAC CGTGTCTCGG ATTGCTCAGG ACATGTTACT GGAACATGGC 
ATTACTCTGG TCATTAATGT AAAGTCACAA GTTTTAGAAA GAATCAGTCG AATGACCCAA 
GGTGATTTAG TGGTGTCCAT GGACCAGCTG CTCACCAAAC CCCACTTGGG CACTTGCCAC 
AAATTTTATA TGCAGATATT TCAGCTGCCT AATGAACAAA CCAAAACACT GATGTTTTTT 
GAAGGCTGTC CACAGCACCT AGGCTGCACA ATCAAGCTCA GAGGAGGCTC TGATTATGAG 
CTGGCTCGAG TTAAGGAGAT CCTAATATTT ATGATCTGTG TAGCTTATCA TTCTCAGCTA 
GAAATCTCTT TTCTCATGGA TGAGTTCGCT ATGCCTCCAA CGTTAATGCA AAGCCCTTCG 
TTCCATCTTC TGACGGAGGG ACGGGGTGAA GAGGGAGCCT CTCAGGAGCA GGTCAGTGGC 
AGCTCCCTTC CTCAGGATCC TGAGTGCCCT CGTGAGGCCC TGTCTTCTGA CGATAGCACT 
TTGTTGGAAT CAAGGACTGT GCTAGAGAAG GGTGAACTAG ACAATAAAAG TATTCCACAA 
GCTGTTGCCT CTTTGAAGCA TCAAGATTAT ACCACCCCCA CTTGCCCAGC AGGTATCCCC 
TGTGCTCTCT TTGCATTGGT ACCAGAGTCA TTGTTGCCTC TCCATATGGA TCAACAGGAT 
GCTGTAGGAA ATGAACAACC AGAGACATCA CAACAAACAG ATGAGCAACA GGATCCCAAA 
AGCCAGATGA AAGCTTTTAG AGACCCTCTA CAGGATGACA CCGGAATGTA TGTTACTGAG 
GAAGTCACCT CCTCTGAAGA TCAACGAAAG ACTTATGCCT TGACATTTAA ACAGGAATTA 
AAAGACGTAA TCCTCTGTAT CTCTCCAGTT ATTACATTCC GTGAACCTTT CCTTTTAACT 
GAAAAGGGGA TGAGATGCTC AACTCGAGAT TATTTTCCAG AGCAGATTTA CTGGTCTCCT 
CTTCTCAACA AAGAGGTGAT GGAAATGGAG AGCAGGAGGA AGAAACAGCT GCTCAGGGAT 
CTCTCTGGAC TTCAGGGCAT GAATGGCAGT GTTCAGGCCA AGTCTATTCA AGTCTTACCC 
TCACATGAAC TAGTGAGCAC CAGGATTGCT GAACATCTGG GTGACAGCCA GACCTTGGGT 
AGAATGCTAG CTGATTATCG AGCTAGAGGA GGAAGAATTC AGTCAAAACA TTTGGACCCT 
TTTGTCCATT CAAAAGATGC ATCATGTACT TCAGGTGGAA AACCAGGAAA CAAAACTGAG 
AGTGATGAAG AGAGGGGATT GATCCCAAGT GATGTAATAT GGCCAGCAAA GGTGGACTGT 
CTGAATCCTG CTAACCACCA GAGGCTCTGT GTGCTCTTCA GCAGCTCTTC TGCCCAGTCC 
AGCAATGCTC CCAGTGCTTG TGTCAGTCCT TGGATTGTAA CAATGGAGTT TTATGGAAAG 
AATGACCTTA CACTGGGAAT ATTTTTAGAA AGATACTGTT TCAGGTCTTC TTACCAGTGT 
CCGAGCATGT TCTGTGACAC CCCCATGGTT CATCACATTC GACGCTTTGT TCATGGCCAA 
GGCTGTGTAC AGATAATTCT GAAGGAGTTG GATTCTCCAG TGCCTGGATA TCAACATACA 
ATTCTCACAT ACTCCTGGTG CAGAATCTGC AAACAAGTAA CACCAGTTGT TGCTCTTTCA 
AATGAATCCT GGTCTATGTC ATTTGCAAAG TACCTTGAAC TTCGATTTTA TGGCCACCAG 
TACACACGCA GAGCCAACGC TGAGCCCTGC GGTCACTCTA TCCACCATGA TTATCACCAG 
TATTTCTCTT ATAACCAGAT GGTGGCATCT TTCAGTTACT CTCCTATTCG GCTTCTTGAA 
GTATGTGTTC CACTACCAAA AATATTCATT AAGCGTCAAG CCCCACTGAA GGTATCTCTT 
CTTCAGGACC TCAAGGACTT TTTTCAGAAG GTTTCACAGG TGTACCTAGC TGTTGATGAG 
AGACTTGCAT CCTTGAAAAC GGATACATTT AGCAAAACCA GAGAGGAAAA GATGGAAGAT 
ATCTTTGCAC AAAAGGAGAT GGAAGAGGGT GAGTTTAAGA ACTGGACAGA GAAGATGCAA 
GCAAGGCTCA TGTCTTCCTC TGTGGATACC CCTCAGCAAC TGCAGTCCAT TTTTGAGTCA 
CTGATTGCCA AGAAGCAAAG CCTCTGTGAG GTGCTCCAGG CGTGGAACAG CAGGTTGCAG 
GACCTCTTCC AGCAGGAAAA AGGTAGAAAG AGGCCTTCAG TTCCTCCCAG TCCTGGGAGA 
CTGAGACAAG GTGAAGAAAG CAAGATAAAT GCAATGGACA CATCTCCAAG GAATATTTCT 
CCAGGACTTC ACAATGGAGA AAAAGAGGAT CGCTTCTTGA CAACCCTGTC CAGCCAGAGC 
TCTACGAGCT CCACCCACCT CCAGCTGCCC ACTCCTCCCG AGGCCCTGGC CGAGCAGGTA 
GTGGGAGGGC CGACTGATCT GGATTCAGCC AGTGGTTCTG AAGATGTATT TGATGGTCAT 
TTGCTGGGAT CCACAGACAG CCAGGTGAAG GAAAAGTCAA CCATGAAAGC CATCTTTGCT 
AATTTGCTTC CAGGAAACAG CTACAATCCC ATTCCATTTC CTTTTGATCC AGATAAACAC 
TACTTAATGT ATGAACATGA ACGGGTGCCC ATTGCTGTCT GTGAGAAAGA GCCCAGCTCC 
ATCATTGCTT TTGCACTCAG TTGTAAAGAA TACCGAAATG CCTTAGAGGA ATTGTCCAAA 
GCGACTCTGT GGAACAGTGC TGAAGAAGGG CTTCCAGCCA ATAGTGCTTT AGATAACAGA 
CCTAAGAGTA GCAGCCCTAT TAGAGTACCT GAAATCAGTG GAGGACAGAC AAACCGCACA 
GGAGAAGCAG AACCTCAGCC AACCAAAAAA GCTTCAGGCA TGTTGTCCTT CTTCAGAGGA 
ACAGCAGGGA AGAGTCCTGA CCTGTCTTCT CAGAAGAGGG AGACCTTACG TGGGGCAGAC 
AGTGCTTACT ACCAGGTTGG GCAGGCTGGC AAGGAGGGGT TGGAGAGTCA AGGCCTGGAG 
CCTCAAGATG AAGTAGACGG AGGAGATACA CAGAAGAAAC AACTCACAAA TCCTCATGTG 
GAACTTCAAT TTTCTGATGC TAATGCCAAG TTTTACTGTC GGCTGTACTA CGCGGGAGAG 
TTCCATAAGA TGCGTGAAGT GATTCTGGGC AGCAGCGAGG AGGATTTCAT TCGCTCCCTT 
TCTCACTCAT CTCCCTGGCA GGCCCGGGGA GGCAAGTCAG GAGCTGCTTT CTATGCCACC 
GAAGATGATA GATTCATTCT GAAGCAAATG CCTCGTTTGG AAGTCCAGTC TTTCCTTGAC 
TTTGCACCAC ACTACTTCAA TTATATCACA AATGCTGTTC AACAAAAGAG GCCCACCGCC 
TTGGCTAAAA TTCTTGGAGT TTACAGAATT GGCTATAAGA ACTCTCAGAA CAACACTGAG 
AAGAAGTTAG ATCTCCTTGT TATGGAAAAT CTTTTCTATG GGAGAAAGAT GGCACAGGTT 
TTTGATTTGA AGGGTTCACT TAGGAATCGA AATGTAAAAA CTGACACTGG GAAAGAGAGC 
TGTGATGTGG TTCTGTTGGA TGAAAACCTC CTAAAGATGG TTCGAGACAA CCCTCTCTAT 
ATTCGTTCCC ATTCCAAATC TGTGCTGAGA ACCTCCATCC ACAGCGACGC CCATTTCCTT 
TCCAGCCACC TCATTATAGA CTATTCTCTG CTGGTTGGGC GAGATGACAC TAGCAATGAG 
CTTGTGGTTG GCATCATAGA TTACATTCGA ACATTTACAT GGGACAAAAA ACTTGAGATG 
GTTGTGAAGT CAACAGGAAT TTTAGGAGGA CAAGGTAAAA TGCCAACTGT GGTCTCTCCA 
GAGTTGTATA GGACTAGATT TTGTGAAGCA ATGGACAAGT ATTTCTTGAT GGTGCCAGAC 
CACTGGACAG GGTTGGATCT GAATTGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Pikfyve ( XM_029476073.1 ) cDNA ORF clone, Mus caroli(Ryukyu mouse) -> XP_029331933.1 Mus caroli phosphoinositide kinase, FYVE-type zinc finger containing (Pikfyve), transcript variant X3, mRNA.

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CloneID	OMv66196
Clone ID Related Accession (Same CDS sequence)	XM_029476073.1
Accession Version	XM_029476073.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6327bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-06-09
Organism	Mus caroli(Ryukyu mouse)
Product	1-phosphatidylinositol 3-phosphate 5-kinase isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_034570.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus caroli Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCACAG ATGACAAGAG TTCCCCGACA CTGGACTCTG CTAATGATTT GCCTCGCTCT 
CCCGCCAGTC CTTCTCACCT CACTCACTTT AAACCCTTGA CTCCTGACCA GGATGAGCCC 
CCCTTCAAGT CAGCATATAG TTCTTTTGTA AACCTTTTTC GCTTTAACAA AGAGCGGGGA 
GAAGGGGGCC AAGGAGAGCA GCAGTCTCCG AGTTCAAGTT GGGCCAGCCC TCAGATCCCT 
TCAAGAACAC AGTCTGTGAG GTCGCCTGTA CCTTATAAAA AACAGCTTAA TGAGGAGCTC 
CACCGGCGCT CTTCAGTGTT AGAGAACACT TTGCCACATC CTCAGGAGAG CACAGACTCC 
AGAAGGAAAG CAGAACCAGC CTGTGGAGGT CATGACCCAC GTACAGCTGT TCAGCTTCGA 
AGCCTCAGCA CAGTATTGAA ACGCCTCAAA GAAATCATGG AAGGAAAAAG CCAGGACAGT 
GACCTGAAGC AATATTGGAT GCCAGATAGC CAGTGTAAAG AGTGCTATGA CTGCAGTGAA 
AAGTTTACAA CATTTAGGCG CAGACACCAT TGCAGACTGT GTGGGCAGAT TTTCTGCAGT 
CGTTGTTGTA ATCAAGAAAT CCCTGGAAAA TTTATGGGCT ATACAGGAGA CCTCCGAGCA 
TGCACCTACT GTAGGAAAAT AGCCTTAAGT TATGCTCATT CCACAGACAG TAATTCCATT 
GGGGAAGACT TGAATGCTCT TTCAGATTCA ACTTGCTCTG TGTCTATACT TGATCCAAGC 
GAACCTCGGA CACCTGTTGG GAGTAGAAAA GCCAGTCGTA ACATATTCTT AGAGGATGAT 
TTAGCCTGGC AAAGTTTGAT TCATCCGGAT TCCTCAAATA GTGCTCTTTC AACAAGACTC 
GTATCTGTTC AAGAGGATGC TGGGAAGTCT CCTGCTCGGA ACAGATCAGC CAGCATTACT 
AATCTGTCAC TGGATCGGTC TGGTTCTCCT ATGGTTCCTT CATATGAGAC ATCTGTCAGT 
CCCCAGGCTA ACCGAAACTA CATTAGGACC GAGACGACTG AGGATGAACG CAAAATTCTT 
CTGGACAGTG CTCAGTTAAA GGATCTGTGG AAGAAAATCT GCCATCACAC CAGTGGGATG 
GAATTTCAAG ATCACCGTTA CTGGTTGAGA ACACATCCCA ACTGCATTGT AGGAAAGGAA 
TTAGTCAACT GGCTAATCAG AAATGGACAC ATCGCTACAA GGGCACAAGC TATAGCAATT 
GGACAAGCAA TGGTTGATGG ACGTTGGTTG GATTGTGTTA GTCATCATGA CCAGCTTTTC 
AGGGACGAAT ATGCGTTGTA TAGACCACTT CAGAGTACAG AATTTTCTGA GACACCTTCT 
CCAGACAGTG ACTCCGTGAA CTCTGTGGAA GGACACTCCG AGCCATCCTG GTTTAAAGAC 
ATAAAATTTG ATGACAGTGA CACAGAACAG ATTGCTGAAG AAGGTGACGA TAATTTGGCT 
AATTCTGCCA GTCCTAGCAA GCGCACCTCA GTCAGCAGTT TCCAGTCCAC AGTGGACAGT 
GACTCAGCCG CTTCTATCAG CCTGAACGTG GAGCTGGACA ACGTGAACTT CCATATCAAG 
AAGCCCTCCA AGTACCCACA TGTGCCCCCT CACCCTGCTG ACCAAAAAGA GTATTTGGTT 
TCTGACACTG GAGGACAGCA GCTCTCAATA AGTGATGCCT TCATCAAAGA GTCCTTATTT 
AATCGACGAG TAGAGGAAAA ATCCAAAGAG CTGCCTTTTA CCCCTTTGGG CTGGCATCAT 
AACAACCTGG AACTCTTGCG AGAGGAGAAT GAGGAGAAGC AAGCCATGGA AAGGCTGCTT 
TCAGCTAATC ATAACCACAT GATGGCCCTA CTCCAGCAAT TGCTTCAAAA CGAGTCATTG 
TCATCGTCTT GGAGGGACAT CATTGTGTCA CTAGTCTGCC AGGTTGTTCA GACAGTCCGA 
CCTGACGTCA AGCACCAGGA TGATGACATG GATATCCGTC AGTTTGTCCA TATCAAGAAG 
ATCCCAGGTG GAAAGAAATT TGATTCTGTG GTTGTCAATG GCTTTGTTTG TACCAAGAAC 
ATTGCACATA AAAAGATGAA TTCCTGTATT AAAAACCCCA AAATCCTTCT GTTGAAGTGT 
TCTATTGAGT ATCTCTATAG AGAAGAAACT AAGTTTACCT GCATTGATCC TATTGTGCTT 
CAGGAAAGGG AATTCTTGAA GAATTATGTT CAACGAATAG TTGATGTTCG ACCCACATTG 
GTTCTTGTTG AGAAAACCGT GTCTCGGATT GCTCAGGACA TGTTACTGGA ACATGGCATT 
ACTCTGGTCA TTAATGTAAA GTCACAAGTT TTAGAAAGAA TCAGTCGAAT GACCCAAGGT 
GATTTAGTGG TGTCCATGGA CCAGCTGCTC ACCAAACCCC ACTTGGGCAC TTGCCACAAA 
TTTTATATGC AGATATTTCA GCTGCCTAAT GAACAAACCA AAACACTGAT GTTTTTTGAA 
GGCTGTCCAC AGCACCTAGG CTGCACAATC AAGCTCAGAG GAGGCTCTGA TTATGAGCTG 
GCTCGAGTTA AGGAGATCCT AATATTTATG ATCTGTGTAG CTTATCATTC TCAGCTAGAA 
ATCTCTTTTC TCATGGATGA GTTCGCTATG CCTCCAACGT TAATGCAAAG CCCTTCGTTC 
CATCTTCTGA CGGAGGGACG GGGTGAAGAG GGAGCCTCTC AGGAGCAGGT CAGTGGCAGC 
TCCCTTCCTC AGGATCCTGA GTGCCCTCGT GAGGCCCTGT CTTCTGACGA TAGCACTTTG 
TTGGAATCAA GGACTGTGCT AGAGAAGGGT GAACTAGACA ATAAAAGTAT TCCACAAGCT 
GTTGCCTCTT TGAAGCATCA AGATTATACC ACCCCCACTT GCCCAGCAGG TATCCCCTGT 
GCTCTCTTTG CATTGGTACC AGAGTCATTG TTGCCTCTCC ATATGGATCA ACAGGATGCT 
GTAGGAAATG AACAACCAGA GACATCACAA CAAACAGATG AGCAACAGGA TCCCAAAAGC 
CAGATGAAAG CTTTTAGAGA CCCTCTACAG GATGACACCG GAATGTATGT TACTGAGGAA 
GTCACCTCCT CTGAAGATCA ACGAAAGACT TATGCCTTGA CATTTAAACA GGAATTAAAA 
GACGTAATCC TCTGTATCTC TCCAGTTATT ACATTCCGTG AACCTTTCCT TTTAACTGAA 
AAGGGGATGA GATGCTCAAC TCGAGATTAT TTTCCAGAGC AGATTTACTG GTCTCCTCTT 
CTCAACAAAG AGGTGATGGA AATGGAGAGC AGGAGGAAGA AACAGCTGCT CAGGGATCTC 
TCTGGACTTC AGGGCATGAA TGGCAGTGTT CAGGCCAAGT CTATTCAAGT CTTACCCTCA 
CATGAACTAG TGAGCACCAG GATTGCTGAA CATCTGGGTG ACAGCCAGAC CTTGGGTAGA 
ATGCTAGCTG ATTATCGAGC TAGAGGAGGA AGAATTCAGT CAAAACATTT GGACCCTTTT 
GTCCATTCAA AAGATGCATC ATGTACTTCA GGTGGAAAAC CAGGAAACAA AACTGAGAGT 
GATGAAGAGA GGGGATTGAT CCCAAGTGAT GTAATATGGC CAGCAAAGGT GGACTGTCTG 
AATCCTGCTA ACCACCAGAG GCTCTGTGTG CTCTTCAGCA GCTCTTCTGC CCAGTCCAGC 
AATGCTCCCA GTGCTTGTGT CAGTCCTTGG ATTGTAACAA TGGAGTTTTA TGGAAAGAAT 
GACCTTACAC TGGGAATATT TTTAGAAAGA TACTGTTTCA GGTCTTCTTA CCAGTGTCCG 
AGCATGTTCT GTGACACCCC CATGGTTCAT CACATTCGAC GCTTTGTTCA TGGCCAAGGC 
TGTGTACAGA TAATTCTGAA GGAGTTGGAT TCTCCAGTGC CTGGATATCA ACATACAATT 
CTCACATACT CCTGGTGCAG AATCTGCAAA CAAGTAACAC CAGTTGTTGC TCTTTCAAAT 
GAATCCTGGT CTATGTCATT TGCAAAGTAC CTTGAACTTC GATTTTATGG CCACCAGTAC 
ACACGCAGAG CCAACGCTGA GCCCTGCGGT CACTCTATCC ACCATGATTA TCACCAGTAT 
TTCTCTTATA ACCAGATGGT GGCATCTTTC AGTTACTCTC CTATTCGGCT TCTTGAAGTA 
TGTGTTCCAC TACCAAAAAT ATTCATTAAG CGTCAAGCCC CACTGAAGGT ATCTCTTCTT 
CAGGACCTCA AGGACTTTTT TCAGAAGGTT TCACAGGTGT ACCTAGCTGT TGATGAGAGA 
CTTGCATCCT TGAAAACGGA TACATTTAGC AAAACCAGAG AGGAAAAGAT GGAAGATATC 
TTTGCACAAA AGGAGATGGA AGAGGGTGAG TTTAAGAACT GGACAGAGAA GATGCAAGCA 
AGGCTCATGT CTTCCTCTGT GGATACCCCT CAGCAACTGC AGTCCATTTT TGAGTCACTG 
ATTGCCAAGA AGCAAAGCCT CTGTGAGGTG CTCCAGGCGT GGAACAGCAG GTTGCAGGAC 
CTCTTCCAGC AGGAAAAAGG TAGAAAGAGG CCTTCAGTTC CTCCCAGTCC TGGGAGACTG 
AGACAAGGTG AAGAAAGCAA GATAAATGCA ATGGACACAT CTCCAAGGAA TATTTCTCCA 
GGACTTCACA ATGGAGAAAA AGAGGATCGC TTCTTGACAA CCCTGTCCAG CCAGAGCTCT 
ACGAGCTCCA CCCACCTCCA GCTGCCCACT CCTCCCGAGG CCCTGGCCGA GCAGGTAGTG 
GGAGGGCCGA CTGATCTGGA TTCAGCCAGT GGTTCTGAAG ATGTATTTGA TGGTCATTTG 
CTGGGATCCA CAGACAGCCA GGTGAAGGAA AAGTCAACCA TGAAAGCCAT CTTTGCTAAT 
TTGCTTCCAG GAAACAGCTA CAATCCCATT CCATTTCCTT TTGATCCAGA TAAACACTAC 
TTAATGTATG AACATGAACG GGTGCCCATT GCTGTCTGTG AGAAAGAGCC CAGCTCCATC 
ATTGCTTTTG CACTCAGTTG TAAAGAATAC CGAAATGCCT TAGAGGAATT GTCCAAAGCG 
ACTCTGTGGA ACAGTGCTGA AGAAGGGCTT CCAGCCAATA GTGCTTTAGA TAACAGACCT 
AAGAGTAGCA GCCCTATTAG AGTACCTGAA ATCAGTGGAG GACAGACAAA CCGCACAGGA 
GAAGCAGAAC CTCAGCCAAC CAAAAAAGCT TCAGGCATGT TGTCCTTCTT CAGAGGAACA 
GCAGGGAAGA GTCCTGACCT GTCTTCTCAG AAGAGGGAGA CCTTACGTGG GGCAGACAGT 
GCTTACTACC AGGTTGGGCA GGCTGGCAAG GAGGGGTTGG AGAGTCAAGG CCTGGAGCCT 
CAAGATGAAG TAGACGGAGG AGATACACAG AAGAAACAAC TCACAAATCC TCATGTGGAA 
CTTCAATTTT CTGATGCTAA TGCCAAGTTT TACTGTCGGC TGTACTACGC GGGAGAGTTC 
CATAAGATGC GTGAAGTGAT TCTGGGCAGC AGCGAGGAGG ATTTCATTCG CTCCCTTTCT 
CACTCATCTC CCTGGCAGGC CCGGGGAGGC AAGTCAGGAG CTGCTTTCTA TGCCACCGAA 
GATGATAGAT TCATTCTGAA GCAAATGCCT CGTTTGGAAG TCCAGTCTTT CCTTGACTTT 
GCACCACACT ACTTCAATTA TATCACAAAT GCTGTTCAAC AAAAGAGGCC CACCGCCTTG 
GCTAAAATTC TTGGAGTTTA CAGAATTGGC TATAAGAACT CTCAGAACAA CACTGAGAAG 
AAGTTAGATC TCCTTGTTAT GGAAAATCTT TTCTATGGGA GAAAGATGGC ACAGGTTTTT 
GATTTGAAGG GTTCACTTAG GAATCGAAAT GTAAAAACTG ACACTGGGAA AGAGAGCTGT 
GATGTGGTTC TGTTGGATGA AAACCTCCTA AAGATGGTTC GAGACAACCC TCTCTATATT 
CGTTCCCATT CCAAATCTGT GCTGAGAACC TCCATCCACA GCGACGCCCA TTTCCTTTCC 
AGCCACCTCA TTATAGACTA TTCTCTGCTG GTTGGGCGAG ATGACACTAG CAATGAGCTT 
GTGGTTGGCA TCATAGATTA CATTCGAACA TTTACATGGG ACAAAAAACT TGAGATGGTT 
GTGAAGTCAA CAGGAATTTT AGGAGGACAA GGTAAAATGC CAACTGTGGT CTCTCCAGAG 
TTGTATAGGA CTAGATTTTG TGAAGCAATG GACAAGTATT TCTTGATGGT GCCAGACCAC 
TGGACAGGGT TGGATCTGAA TTGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_029331933.1
CDS	164..6490
Translation	MATDDKSSPTLDSANDLPRSPASPSHLTHFKPLTPDQDEPPFKSAYSSFVNLFRFNKERGEGGQGEQQSPSSSWASPQIPSRTQSVRSPVPYKKQLNEELHRRSSVLENTLPHPQESTDSRRKAEPACGGHDPRTAVQLRSLSTVLKRLKEIMEGKSQDSDLKQYWMPDSQCKECYDCSEKFTTFRRRHHCRLCGQIFCSRCCNQEIPGKFMGYTGDLRACTYCRKIALSYAHSTDSNSIGEDLNALSDSTCSVSILDPSEPRTPVGSRKASRNIFLEDDLAWQSLIHPDSSNSALSTRLVSVQEDAGKSPARNRSASITNLSLDRSGSPMVPSYETSVSPQANRNYIRTETTEDERKILLDSAQLKDLWKKICHHTSGMEFQDHRYWLRTHPNCIVGKELVNWLIRNGHIATRAQAIAIGQAMVDGRWLDCVSHHDQLFRDEYALYRPLQSTEFSETPSPDSDSVNSVEGHSEPSWFKDIKFDDSDTEQIAEEGDDNLANSASPSKRTSVSSFQSTVDSDSAASISLNVELDNVNFHIKKPSKYPHVPPHPADQKEYLVSDTGGQQLSISDAFIKESLFNRRVEEKSKELPFTPLGWHHNNLELLREENEEKQAMERLLSANHNHMMALLQQLLQNESLSSSWRDIIVSLVCQVVQTVRPDVKHQDDDMDIRQFVHIKKIPGGKKFDSVVVNGFVCTKNIAHKKMNSCIKNPKILLLKCSIEYLYREETKFTCIDPIVLQEREFLKNYVQRIVDVRPTLVLVEKTVSRIAQDMLLEHGITLVINVKSQVLERISRMTQGDLVVSMDQLLTKPHLGTCHKFYMQIFQLPNEQTKTLMFFEGCPQHLGCTIKLRGGSDYELARVKEILIFMICVAYHSQLEISFLMDEFAMPPTLMQSPSFHLLTEGRGEEGASQEQVSGSSLPQDPECPREALSSDDSTLLESRTVLEKGELDNKSIPQAVASLKHQDYTTPTCPAGIPCALFALVPESLLPLHMDQQDAVGNEQPETSQQTDEQQDPKSQMKAFRDPLQDDTGMYVTEEVTSSEDQRKTYALTFKQELKDVILCISPVITFREPFLLTEKGMRCSTRDYFPEQIYWSPLLNKEVMEMESRRKKQLLRDLSGLQGMNGSVQAKSIQVLPSHELVSTRIAEHLGDSQTLGRMLADYRARGGRIQSKHLDPFVHSKDASCTSGGKPGNKTESDEERGLIPSDVIWPAKVDCLNPANHQRLCVLFSSSSAQSSNAPSACVSPWIVTMEFYGKNDLTLGIFLERYCFRSSYQCPSMFCDTPMVHHIRRFVHGQGCVQIILKELDSPVPGYQHTILTYSWCRICKQVTPVVALSNESWSMSFAKYLELRFYGHQYTRRANAEPCGHSIHHDYHQYFSYNQMVASFSYSPIRLLEVCVPLPKIFIKRQAPLKVSLLQDLKDFFQKVSQVYLAVDERLASLKTDTFSKTREEKMEDIFAQKEMEEGEFKNWTEKMQARLMSSSVDTPQQLQSIFESLIAKKQSLCEVLQAWNSRLQDLFQQEKGRKRPSVPPSPGRLRQGEESKINAMDTSPRNISPGLHNGEKEDRFLTTLSSQSSTSSTHLQLPTPPEALAEQVVGGPTDLDSASGSEDVFDGHLLGSTDSQVKEKSTMKAIFANLLPGNSYNPIPFPFDPDKHYLMYEHERVPIAVCEKEPSSIIAFALSCKEYRNALEELSKATLWNSAEEGLPANSALDNRPKSSSPIRVPEISGGQTNRTGEAEPQPTKKASGMLSFFRGTAGKSPDLSSQKRETLRGADSAYYQVGQAGKEGLESQGLEPQDEVDGGDTQKKQLTNPHVELQFSDANAKFYCRLYYAGEFHKMREVILGSSEEDFIRSLSHSSPWQARGGKSGAAFYATEDDRFILKQMPRLEVQSFLDFAPHYFNYITNAVQQKRPTALAKILGVYRIGYKNSQNNTEKKLDLLVMENLFYGRKMAQVFDLKGSLRNRNVKTDTGKESCDVVLLDENLLKMVRDNPLYIRSHSKSVLRTSIHSDAHFLSSHLIIDYSLLVGRDDTSNELVVGIIDYIRTFTWDKKLEMVVKSTGILGGQGKMPTVVSPELYRTRFCEAMDKYFLMVPDHWTGLDLNC

Target ORF information:

RefSeq Version	XM_029476073.1
Organism	Mus caroli(Ryukyu mouse)
Definition	Mus caroli phosphoinositide kinase, FYVE-type zinc finger containing (Pikfyve), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_029476073.1

ORF Insert Sequence:

ATGGCCACAG ATGACAAGAG TTCCCCGACA CTGGACTCTG CTAATGATTT GCCTCGCTCT 
CCCGCCAGTC CTTCTCACCT CACTCACTTT AAACCCTTGA CTCCTGACCA GGATGAGCCC 
CCCTTCAAGT CAGCATATAG TTCTTTTGTA AACCTTTTTC GCTTTAACAA AGAGCGGGGA 
GAAGGGGGCC AAGGAGAGCA GCAGTCTCCG AGTTCAAGTT GGGCCAGCCC TCAGATCCCT 
TCAAGAACAC AGTCTGTGAG GTCGCCTGTA CCTTATAAAA AACAGCTTAA TGAGGAGCTC 
CACCGGCGCT CTTCAGTGTT AGAGAACACT TTGCCACATC CTCAGGAGAG CACAGACTCC 
AGAAGGAAAG CAGAACCAGC CTGTGGAGGT CATGACCCAC GTACAGCTGT TCAGCTTCGA 
AGCCTCAGCA CAGTATTGAA ACGCCTCAAA GAAATCATGG AAGGAAAAAG CCAGGACAGT 
GACCTGAAGC AATATTGGAT GCCAGATAGC CAGTGTAAAG AGTGCTATGA CTGCAGTGAA 
AAGTTTACAA CATTTAGGCG CAGACACCAT TGCAGACTGT GTGGGCAGAT TTTCTGCAGT 
CGTTGTTGTA ATCAAGAAAT CCCTGGAAAA TTTATGGGCT ATACAGGAGA CCTCCGAGCA 
TGCACCTACT GTAGGAAAAT AGCCTTAAGT TATGCTCATT CCACAGACAG TAATTCCATT 
GGGGAAGACT TGAATGCTCT TTCAGATTCA ACTTGCTCTG TGTCTATACT TGATCCAAGC 
GAACCTCGGA CACCTGTTGG GAGTAGAAAA GCCAGTCGTA ACATATTCTT AGAGGATGAT 
TTAGCCTGGC AAAGTTTGAT TCATCCGGAT TCCTCAAATA GTGCTCTTTC AACAAGACTC 
GTATCTGTTC AAGAGGATGC TGGGAAGTCT CCTGCTCGGA ACAGATCAGC CAGCATTACT 
AATCTGTCAC TGGATCGGTC TGGTTCTCCT ATGGTTCCTT CATATGAGAC ATCTGTCAGT 
CCCCAGGCTA ACCGAAACTA CATTAGGACC GAGACGACTG AGGATGAACG CAAAATTCTT 
CTGGACAGTG CTCAGTTAAA GGATCTGTGG AAGAAAATCT GCCATCACAC CAGTGGGATG 
GAATTTCAAG ATCACCGTTA CTGGTTGAGA ACACATCCCA ACTGCATTGT AGGAAAGGAA 
TTAGTCAACT GGCTAATCAG AAATGGACAC ATCGCTACAA GGGCACAAGC TATAGCAATT 
GGACAAGCAA TGGTTGATGG ACGTTGGTTG GATTGTGTTA GTCATCATGA CCAGCTTTTC 
AGGGACGAAT ATGCGTTGTA TAGACCACTT CAGAGTACAG AATTTTCTGA GACACCTTCT 
CCAGACAGTG ACTCCGTGAA CTCTGTGGAA GGACACTCCG AGCCATCCTG GTTTAAAGAC 
ATAAAATTTG ATGACAGTGA CACAGAACAG ATTGCTGAAG AAGGTGACGA TAATTTGGCT 
AATTCTGCCA GTCCTAGCAA GCGCACCTCA GTCAGCAGTT TCCAGTCCAC AGTGGACAGT 
GACTCAGCCG CTTCTATCAG CCTGAACGTG GAGCTGGACA ACGTGAACTT CCATATCAAG 
AAGCCCTCCA AGTACCCACA TGTGCCCCCT CACCCTGCTG ACCAAAAAGA GTATTTGGTT 
TCTGACACTG GAGGACAGCA GCTCTCAATA AGTGATGCCT TCATCAAAGA GTCCTTATTT 
AATCGACGAG TAGAGGAAAA ATCCAAAGAG CTGCCTTTTA CCCCTTTGGG CTGGCATCAT 
AACAACCTGG AACTCTTGCG AGAGGAGAAT GAGGAGAAGC AAGCCATGGA AAGGCTGCTT 
TCAGCTAATC ATAACCACAT GATGGCCCTA CTCCAGCAAT TGCTTCAAAA CGAGTCATTG 
TCATCGTCTT GGAGGGACAT CATTGTGTCA CTAGTCTGCC AGGTTGTTCA GACAGTCCGA 
CCTGACGTCA AGCACCAGGA TGATGACATG GATATCCGTC AGTTTGTCCA TATCAAGAAG 
ATCCCAGGTG GAAAGAAATT TGATTCTGTG GTTGTCAATG GCTTTGTTTG TACCAAGAAC 
ATTGCACATA AAAAGATGAA TTCCTGTATT AAAAACCCCA AAATCCTTCT GTTGAAGTGT 
TCTATTGAGT ATCTCTATAG AGAAGAAACT AAGTTTACCT GCATTGATCC TATTGTGCTT 
CAGGAAAGGG AATTCTTGAA GAATTATGTT CAACGAATAG TTGATGTTCG ACCCACATTG 
GTTCTTGTTG AGAAAACCGT GTCTCGGATT GCTCAGGACA TGTTACTGGA ACATGGCATT 
ACTCTGGTCA TTAATGTAAA GTCACAAGTT TTAGAAAGAA TCAGTCGAAT GACCCAAGGT 
GATTTAGTGG TGTCCATGGA CCAGCTGCTC ACCAAACCCC ACTTGGGCAC TTGCCACAAA 
TTTTATATGC AGATATTTCA GCTGCCTAAT GAACAAACCA AAACACTGAT GTTTTTTGAA 
GGCTGTCCAC AGCACCTAGG CTGCACAATC AAGCTCAGAG GAGGCTCTGA TTATGAGCTG 
GCTCGAGTTA AGGAGATCCT AATATTTATG ATCTGTGTAG CTTATCATTC TCAGCTAGAA 
ATCTCTTTTC TCATGGATGA GTTCGCTATG CCTCCAACGT TAATGCAAAG CCCTTCGTTC 
CATCTTCTGA CGGAGGGACG GGGTGAAGAG GGAGCCTCTC AGGAGCAGGT CAGTGGCAGC 
TCCCTTCCTC AGGATCCTGA GTGCCCTCGT GAGGCCCTGT CTTCTGACGA TAGCACTTTG 
TTGGAATCAA GGACTGTGCT AGAGAAGGGT GAACTAGACA ATAAAAGTAT TCCACAAGCT 
GTTGCCTCTT TGAAGCATCA AGATTATACC ACCCCCACTT GCCCAGCAGG TATCCCCTGT 
GCTCTCTTTG CATTGGTACC AGAGTCATTG TTGCCTCTCC ATATGGATCA ACAGGATGCT 
GTAGGAAATG AACAACCAGA GACATCACAA CAAACAGATG AGCAACAGGA TCCCAAAAGC 
CAGATGAAAG CTTTTAGAGA CCCTCTACAG GATGACACCG GAATGTATGT TACTGAGGAA 
GTCACCTCCT CTGAAGATCA ACGAAAGACT TATGCCTTGA CATTTAAACA GGAATTAAAA 
GACGTAATCC TCTGTATCTC TCCAGTTATT ACATTCCGTG AACCTTTCCT TTTAACTGAA 
AAGGGGATGA GATGCTCAAC TCGAGATTAT TTTCCAGAGC AGATTTACTG GTCTCCTCTT 
CTCAACAAAG AGGTGATGGA AATGGAGAGC AGGAGGAAGA AACAGCTGCT CAGGGATCTC 
TCTGGACTTC AGGGCATGAA TGGCAGTGTT CAGGCCAAGT CTATTCAAGT CTTACCCTCA 
CATGAACTAG TGAGCACCAG GATTGCTGAA CATCTGGGTG ACAGCCAGAC CTTGGGTAGA 
ATGCTAGCTG ATTATCGAGC TAGAGGAGGA AGAATTCAGT CAAAACATTT GGACCCTTTT 
GTCCATTCAA AAGATGCATC ATGTACTTCA GGTGGAAAAC CAGGAAACAA AACTGAGAGT 
GATGAAGAGA GGGGATTGAT CCCAAGTGAT GTAATATGGC CAGCAAAGGT GGACTGTCTG 
AATCCTGCTA ACCACCAGAG GCTCTGTGTG CTCTTCAGCA GCTCTTCTGC CCAGTCCAGC 
AATGCTCCCA GTGCTTGTGT CAGTCCTTGG ATTGTAACAA TGGAGTTTTA TGGAAAGAAT 
GACCTTACAC TGGGAATATT TTTAGAAAGA TACTGTTTCA GGTCTTCTTA CCAGTGTCCG 
AGCATGTTCT GTGACACCCC CATGGTTCAT CACATTCGAC GCTTTGTTCA TGGCCAAGGC 
TGTGTACAGA TAATTCTGAA GGAGTTGGAT TCTCCAGTGC CTGGATATCA ACATACAATT 
CTCACATACT CCTGGTGCAG AATCTGCAAA CAAGTAACAC CAGTTGTTGC TCTTTCAAAT 
GAATCCTGGT CTATGTCATT TGCAAAGTAC CTTGAACTTC GATTTTATGG CCACCAGTAC 
ACACGCAGAG CCAACGCTGA GCCCTGCGGT CACTCTATCC ACCATGATTA TCACCAGTAT 
TTCTCTTATA ACCAGATGGT GGCATCTTTC AGTTACTCTC CTATTCGGCT TCTTGAAGTA 
TGTGTTCCAC TACCAAAAAT ATTCATTAAG CGTCAAGCCC CACTGAAGGT ATCTCTTCTT 
CAGGACCTCA AGGACTTTTT TCAGAAGGTT TCACAGGTGT ACCTAGCTGT TGATGAGAGA 
CTTGCATCCT TGAAAACGGA TACATTTAGC AAAACCAGAG AGGAAAAGAT GGAAGATATC 
TTTGCACAAA AGGAGATGGA AGAGGGTGAG TTTAAGAACT GGACAGAGAA GATGCAAGCA 
AGGCTCATGT CTTCCTCTGT GGATACCCCT CAGCAACTGC AGTCCATTTT TGAGTCACTG 
ATTGCCAAGA AGCAAAGCCT CTGTGAGGTG CTCCAGGCGT GGAACAGCAG GTTGCAGGAC 
CTCTTCCAGC AGGAAAAAGG TAGAAAGAGG CCTTCAGTTC CTCCCAGTCC TGGGAGACTG 
AGACAAGGTG AAGAAAGCAA GATAAATGCA ATGGACACAT CTCCAAGGAA TATTTCTCCA 
GGACTTCACA ATGGAGAAAA AGAGGATCGC TTCTTGACAA CCCTGTCCAG CCAGAGCTCT 
ACGAGCTCCA CCCACCTCCA GCTGCCCACT CCTCCCGAGG CCCTGGCCGA GCAGGTAGTG 
GGAGGGCCGA CTGATCTGGA TTCAGCCAGT GGTTCTGAAG ATGTATTTGA TGGTCATTTG 
CTGGGATCCA CAGACAGCCA GGTGAAGGAA AAGTCAACCA TGAAAGCCAT CTTTGCTAAT 
TTGCTTCCAG GAAACAGCTA CAATCCCATT CCATTTCCTT TTGATCCAGA TAAACACTAC 
TTAATGTATG AACATGAACG GGTGCCCATT GCTGTCTGTG AGAAAGAGCC CAGCTCCATC 
ATTGCTTTTG CACTCAGTTG TAAAGAATAC CGAAATGCCT TAGAGGAATT GTCCAAAGCG 
ACTCTGTGGA ACAGTGCTGA AGAAGGGCTT CCAGCCAATA GTGCTTTAGA TAACAGACCT 
AAGAGTAGCA GCCCTATTAG AGTACCTGAA ATCAGTGGAG GACAGACAAA CCGCACAGGA 
GAAGCAGAAC CTCAGCCAAC CAAAAAAGCT TCAGGCATGT TGTCCTTCTT CAGAGGAACA 
GCAGGGAAGA GTCCTGACCT GTCTTCTCAG AAGAGGGAGA CCTTACGTGG GGCAGACAGT 
GCTTACTACC AGGTTGGGCA GGCTGGCAAG GAGGGGTTGG AGAGTCAAGG CCTGGAGCCT 
CAAGATGAAG TAGACGGAGG AGATACACAG AAGAAACAAC TCACAAATCC TCATGTGGAA 
CTTCAATTTT CTGATGCTAA TGCCAAGTTT TACTGTCGGC TGTACTACGC GGGAGAGTTC 
CATAAGATGC GTGAAGTGAT TCTGGGCAGC AGCGAGGAGG ATTTCATTCG CTCCCTTTCT 
CACTCATCTC CCTGGCAGGC CCGGGGAGGC AAGTCAGGAG CTGCTTTCTA TGCCACCGAA 
GATGATAGAT TCATTCTGAA GCAAATGCCT CGTTTGGAAG TCCAGTCTTT CCTTGACTTT 
GCACCACACT ACTTCAATTA TATCACAAAT GCTGTTCAAC AAAAGAGGCC CACCGCCTTG 
GCTAAAATTC TTGGAGTTTA CAGAATTGGC TATAAGAACT CTCAGAACAA CACTGAGAAG 
AAGTTAGATC TCCTTGTTAT GGAAAATCTT TTCTATGGGA GAAAGATGGC ACAGGTTTTT 
GATTTGAAGG GTTCACTTAG GAATCGAAAT GTAAAAACTG ACACTGGGAA AGAGAGCTGT 
GATGTGGTTC TGTTGGATGA AAACCTCCTA AAGATGGTTC GAGACAACCC TCTCTATATT 
CGTTCCCATT CCAAATCTGT GCTGAGAACC TCCATCCACA GCGACGCCCA TTTCCTTTCC 
AGCCACCTCA TTATAGACTA TTCTCTGCTG GTTGGGCGAG ATGACACTAG CAATGAGCTT 
GTGGTTGGCA TCATAGATTA CATTCGAACA TTTACATGGG ACAAAAAACT TGAGATGGTT 
GTGAAGTCAA CAGGAATTTT AGGAGGACAA GGTAAAATGC CAACTGTGGT CTCTCCAGAG 
TTGTATAGGA CTAGATTTTG TGAAGCAATG GACAAGTATT TCTTGATGGT GCCAGACCAC 
TGGACAGGGT TGGATCTGAA TTGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Pikfyve ( XM_029476086.1 ) cDNA ORF clone, Mus caroli(Ryukyu mouse) -> XP_029331946.1 Mus caroli phosphoinositide kinase, FYVE-type zinc finger containing (Pikfyve), transcript variant X6, mRNA.

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CloneID	OMv66197
Clone ID Related Accession (Same CDS sequence)	XM_029476086.1
Accession Version	XM_029476086.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6246bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-06-09
Organism	Mus caroli(Ryukyu mouse)
Product	1-phosphatidylinositol 3-phosphate 5-kinase isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_034570.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus caroli Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCACAG ATGACAAGAG TTCCCCGACA CTGGACTCTG CTAATGATTT GCCTCGCTCT 
CCCGCCAGTC CTTCTCACCT CACTCACTTT AAACCCTTGA CTCCTGACCA GGATGAGCCC 
CCCTTCAAGT CAGCATATAG TTCTTTTGTA AACCTTTTTC GCTTTAACAA AGAGCGGGGA 
GAAGGGGGCC AAGGAGAGCA GCAGTCTCCG AGTTCAAGTT GGGCCAGCCC TCAGATCCCT 
TCAAGAACAC AGTCTGTGAG GTCGCCTGTA CCTTATAAAA AACAGCTTAA TGAGGAGCTC 
CACCGGCGCT CTTCAGTGTT AGCAGAGAAC ACTTTGCCAC ATCCTCAGGA GAGCACAGAC 
TCCAGAAGGA AAGCAGAACC AGCCTGTGGA GGTCATGACC CACGTACAGC TGTTCAGCTT 
CGAAGCCTCA GCACAGTATT GAAACGCCTC AAAGAAATCA TGGAAGGAAA AAGCCAGGAC 
AGTGACCTGA AGCAATATTG GATGCCAGAT AGCCAGTGTA AAGAGTGCTA TGACTGCAGT 
GAAAAGTTTA CAACATTTAG GCGCAGACAC CATTGCAGAC TGTGTGGGCA GATTTTCTGC 
AGTCGTTGTT GTAATCAAGA AATCCCTGGA AAATTTATGG GCTATACAGG AGACCTCCGA 
GCATGCACCT ACTGTAGGAA AATAGCCTTA AGTTATGCTC ATTCCACAGA CAGTAATTCC 
ATTGGGGAAG ACTTGAATGC TCTTTCAGAT TCAACTTGCT CTGTGTCTAT ACTTGATCCA 
AGCGAACCTC GGACACCTGT TGGGAGTAGA AAAGCCAGTC GTAACATATT CTTAGAGGAT 
GATTTAGCCT GGCAAAGTTT GATTCATCCG GATTCCTCAA ATAGTGCTCT TTCAACAAGA 
CTCGTATCTG TTCAAGAGGA TGCTGGGAAG TCTCCTGCTC GGAACAGATC AGCCAGCATT 
ACTAATCTGT CACTGGATCG GTCTGGTTCT CCTATGGTTC CTTCATATGA GACATCTGTC 
AGTCCCCAGG CTAACCGAAA CTACATTAGG ACCGAGACGA CTGAGGATGA ACGCAAAATT 
CTTCTGGACA GTGCTCAGTT AAAGGATCTG TGGAAGAAAA TCTGCCATCA CACCAGTGGG 
ATGGAATTTC AAGATCACCG TTACTGGTTG AGAACACATC CCAACTGCAT TGTAGGAAAG 
GAATTAGTCA ACTGGCTAAT CAGAAATGGA CACATCGCTA CAAGGGCACA AGCTATAGCA 
ATTGGACAAG CAATGGTTGA TGGACGTTGG TTGGATTGTG TTAGTCATCA TGACCAGCTT 
TTCAGGGACG AATATGCGTT GTATAGACCA CTTCAGAGTA CAGAATTTTC TGAGACACCT 
TCTCCAGACA GTGACTCCGT GAACTCTGTG GAAGGACACT CCGAGCCATC CTGGTTTAAA 
GACATAAAAT TTGATGACAG TGACACAGAA CAGATTGCTG AAGAAGGTGA CGATAATTTG 
GCTAATTCTG CCAGTCCTAG CAAGCGCACC TCAGTCAGCA GTTTCCAGTC CACAGTGGAC 
AGTGACTCAG CCGCTTCTAT CAGCCTGAAC GTGGAGCTGG ACAACGTGAA CTTCCATATC 
AAGAAGCCCT CCAAGTACCC ACATGTGCCC CCTCACCCTG CTGACCAAAA AGAGTATTTG 
GTTTCTGACA CTGGAGGACA GCAGCTCTCA ATAAGTGATG CCTTCATCAA AGAGTCCTTA 
TTTAATCGAC GAGTAGAGGA AAAATCCAAA GAGCTGCCTT TTACCCCTTT GGGCTGGCAT 
CATAACAACC TGGAACTCTT GCGAGAGGAG AATGAGGAGA AGCAAGCCAT GGAAAGGCTG 
CTTTCAGCTA ATCATAACCA CATGATGGCC CTACTCCAGC AATTGCTTCA AAACGAGTCA 
TTGTCATCGT CTTGGAGGGA CATCATTGTG TCACTAGTCT GCCAGGTTGT TCAGACAGTC 
CGACCTGACG TCAAGCACCA GGATGATGAC ATGGATATCC GTCAGTTTGT CCATATCAAG 
AAGATCCCAG GTGGAAAGAA ATTTGATTCT GTGGTTGTCA ATGGCTTTGT TTGTACCAAG 
AACATTGCAC ATAAAAAGAT GAATTCCTGT ATTAAAAACC CCAAAATCCT TCTGTTGAAG 
TGTTCTATTG AGTATCTCTA TAGAGAAGAA ACTAAGTTTA CCTGCATTGA TCCTATTGTG 
CTTCAGGAAA GGGAATTCTT GAAGAATTAT GTTCAACGAA TAGTTGATGT TCGACCCACA 
TTGGTTCTTG TTGAGAAAAC CGTGTCTCGG ATTGCTCAGG ACATGTTACT GGAACATGGC 
ATTACTCTGG TCATTAATGT AAAGTCACAA GTTTTAGAAA GAATCAGTCG AATGACCCAA 
GGTGATTTAG TGGTGTCCAT GGACCAGCTG CTCACCAAAC CCCACTTGGG CACTTGCCAC 
AAATTTTATA TGCAGATATT TCAGCTGCCT AATGAACAAA CCAAAACACT GATGTTTTTT 
GAAGGCTGTC CACAGCACCT AGGCTGCACA ATCAAGCTCA GAGGAGGCTC TGATTATGAG 
CTGGCTCGAG TTAAGGAGAT CCTAATATTT ATGATCTGTG TAGCTTATCA TTCTCAGCTA 
GAAATCTCTT TTCTCATGGA TGAGTTCGCT ATGCCTCCAA CGTTAATGCA AAGCCCTTCG 
TTCCATCTTC TGACGGAGGG ACGGGGTGAA GAGGGAGCCT CTCAGGAGCA GGTCAGTGGC 
AGCTCCCTTC CTCAGGATCC TGAGTGCCCT CGTGAGGCCC TGTCTTCTGA CGATAGCACT 
TTGTTGGAAT CAAGGACTGT GCTAGAGAAG GGTGAACTAG ACAATAAAAG TATTCCACAA 
GCTGTTGCCT CTTTGAAGCA TCAAGATTAT ACCACCCCCA CTTGCCCAGC AGGTATCCCC 
TGTGCTCTCT TTGCATTGGT ACCAGAGTCA TTGTTGCCTC TCCATATGGA TCAACAGGAT 
GCTGTAGGAA ATGAACAACC AGAGACATCA CAACAAACAG ATGAGCAACA GGATCCCAAA 
AGCCAGATGA AAGCTTTTAG AGACCCTCTA CAGGATGACA CCGGAATGTA TGTTACTGAG 
GAAGTCACCT CCTCTGAAGA TCAACGAAAG ACTTATGCCT TGACATTTAA ACAGGAATTA 
AAAGACGTAA TCCTCTGTAT CTCTCCAGTT ATTACATTCC GTGAACCTTT CCTTTTAACT 
GAAAAGGGGA TGAGATGCTC AACTCGAGAT TATTTTCCAG AGCAGATTTA CTGGTCTCCT 
CTTCTCAACA AAGAGGTGAT GGAAATGGAG AGCAGGAGGA AGAAACAGCT GCTCAGGGAT 
CTCTCTGGAC TTCAGGGCAT GAATGGCAGT GTTCAGGCCA AGTCTATTCA AGTCTTACCC 
TCACATGAAC TAGTGAGCAC CAGGATTGCT GAACATCTGG GTGACAGCCA GACCTTGGGT 
AGAATGCTAG CTGATTATCG AGCTAGAGGA GGAAGAATTC AGTCAAAACA TTTGGACCCT 
TTTGTCCATT CAAAAGATGC ATCATGTACT TCAGGTGGAA AACCAGGAAA CAAAACTGAG 
AGTGATGAAG AGAGGGGATT GATCCCAAGT GATGTAATAT GGCCAGCAAA GGTGGACTGT 
CTGAATCCTG CTAACCACCA GAGGCTCTGT GTGCTCTTCA GCAGCTCTTC TGCCCAGTCC 
AGCAATGCTC CCAGTGCTTG TGTCAGTCCT TGGATTGTAA CAATGGAGTT TTATGGAAAG 
AATGACCTTA CACTGGGAAT ATTTTTAGAA AGATACTGTT TCAGGTCTTC TTACCAGTGT 
CCGAGCATGT TCTGTGACAC CCCCATGGTT CATCACATTC GACGCTTTGT TCATGGCCAA 
GGCTGTGTAC AGATAATTCT GAAGGAGTTG GATTCTCCAG TGCCTGGATA TCAACATACA 
ATTCTCACAT ACTCCTGGTG CAGAATCTGC AAACAAGTAA CACCAGTTGT TGCTCTTTCA 
AATGAATCCT GGTCTATGTC ATTTGCAAAG TACCTTGAAC TTCGATTTTA TGGCCACCAG 
TACACACGCA GAGCCAACGC TGAGCCCTGC GGTCACTCTA TCCACCATGA TTATCACCAG 
TATTTCTCTT ATAACCAGAT GGTGGCATCT TTCAGTTACT CTCCTATTCG GCTTCTTGAA 
GTATGTGTTC CACTACCAAA AATATTCATT AAGCGTCAAG CCCCACTGAA GGTATCTCTT 
CTTCAGGACC TCAAGGACTT TTTTCAGAAG GTTTCACAGG TGTACCTAGC TGTTGATGAG 
AGACTTGCAT CCTTGAAAAC GGATACATTT AGCAAAACCA GAGAGGAAAA GATGGAAGAT 
ATCTTTGCAC AAAAGGAGAT GGAAGAGGGT GAGTTTAAGA ACTGGACAGA GAAGATGCAA 
GCAAGGCTCA TGTCTTCCTC TGTGGATACC CCTCAGCAAC TGCAGTCCAT TTTTGAGTCA 
CTGATTGCCA AGAAGCAAAG CCTCTGTGAG GTGCTCCAGG CGTGGAACAG CAGGTTGCAG 
GACCTCTTCC AGCAGGAAAA AGGTAGAAAG AGGCCTTCAG TTCCTCCCAG TCCTGGGAGA 
CTGAGACAAG GTGAAGAAAG CAAGATAAAT GCAATGGACA CATCTCCAAG GAATATTTCT 
CCAGGACTTC ACAATGGAGA AAAAGAGGAT CGCTTCTTGA CAACCCTGTC CAGCCAGAGC 
TCTACGAGCT CCACCCACCT CCAGCTGCCC ACTCCTCCCG AGGCCCTGGC CGAGCAGGTA 
GTGGGAGGGC CGACTGATCT GGATTCAGCC AGTGGTTCTG AAGATGTATT TGATGGTCAT 
TTGCTGGGAT CCACAGACAG CCAGGTGAAG GAAAAGTCAA CCATGAAAGC CATCTTTGCT 
AATTTGCTTC CAGGAAACAG CTACAATCCC ATTCCATTTC CTTTTGATCC AGATAAACAC 
TACTTAATGT ATGAACATGA ACGGGTGCCC ATTGCTGTCT GTGAGAAAGA GCCCAGCTCC 
ATCATTGCTT TTGCACTCAG TGCTTTAGAT AACAGACCTA AGAGTAGCAG CCCTATTAGA 
GTACCTGAAA TCAGTGGAGG ACAGACAAAC CGCACAGGAG AAGCAGAACC TCAGCCAACC 
AAAAAAGCTT CAGGCATGTT GTCCTTCTTC AGAGGAACAG CAGGGAAGAG TCCTGACCTG 
TCTTCTCAGA AGAGGGAGAC CTTACGTGGG GCAGACAGTG CTTACTACCA GGTTGGGCAG 
GCTGGCAAGG AGGGGTTGGA GAGTCAAGGC CTGGAGCCTC AAGATGAAGT AGACGGAGGA 
GATACACAGA AGAAACAACT CACAAATCCT CATGTGGAAC TTCAATTTTC TGATGCTAAT 
GCCAAGTTTT ACTGTCGGCT GTACTACGCG GGAGAGTTCC ATAAGATGCG TGAAGTGATT 
CTGGGCAGCA GCGAGGAGGA TTTCATTCGC TCCCTTTCTC ACTCATCTCC CTGGCAGGCC 
CGGGGAGGCA AGTCAGGAGC TGCTTTCTAT GCCACCGAAG ATGATAGATT CATTCTGAAG 
CAAATGCCTC GTTTGGAAGT CCAGTCTTTC CTTGACTTTG CACCACACTA CTTCAATTAT 
ATCACAAATG CTGTTCAACA AAAGAGGCCC ACCGCCTTGG CTAAAATTCT TGGAGTTTAC 
AGAATTGGCT ATAAGAACTC TCAGAACAAC ACTGAGAAGA AGTTAGATCT CCTTGTTATG 
GAAAATCTTT TCTATGGGAG AAAGATGGCA CAGGTTTTTG ATTTGAAGGG TTCACTTAGG 
AATCGAAATG TAAAAACTGA CACTGGGAAA GAGAGCTGTG ATGTGGTTCT GTTGGATGAA 
AACCTCCTAA AGATGGTTCG AGACAACCCT CTCTATATTC GTTCCCATTC CAAATCTGTG 
CTGAGAACCT CCATCCACAG CGACGCCCAT TTCCTTTCCA GCCACCTCAT TATAGACTAT 
TCTCTGCTGG TTGGGCGAGA TGACACTAGC AATGAGCTTG TGGTTGGCAT CATAGATTAC 
ATTCGAACAT TTACATGGGA CAAAAAACTT GAGATGGTTG TGAAGTCAAC AGGAATTTTA 
GGAGGACAAG GTAAAATGCC AACTGTGGTC TCTCCAGAGT TGTATAGGAC TAGATTTTGT 
GAAGCAATGG ACAAGTATTT CTTGATGGTG CCAGACCACT GGACAGGGTT GGATCTGAAT 
TGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_029331946.1
CDS	164..6409
Translation	MATDDKSSPTLDSANDLPRSPASPSHLTHFKPLTPDQDEPPFKSAYSSFVNLFRFNKERGEGGQGEQQSPSSSWASPQIPSRTQSVRSPVPYKKQLNEELHRRSSVLAENTLPHPQESTDSRRKAEPACGGHDPRTAVQLRSLSTVLKRLKEIMEGKSQDSDLKQYWMPDSQCKECYDCSEKFTTFRRRHHCRLCGQIFCSRCCNQEIPGKFMGYTGDLRACTYCRKIALSYAHSTDSNSIGEDLNALSDSTCSVSILDPSEPRTPVGSRKASRNIFLEDDLAWQSLIHPDSSNSALSTRLVSVQEDAGKSPARNRSASITNLSLDRSGSPMVPSYETSVSPQANRNYIRTETTEDERKILLDSAQLKDLWKKICHHTSGMEFQDHRYWLRTHPNCIVGKELVNWLIRNGHIATRAQAIAIGQAMVDGRWLDCVSHHDQLFRDEYALYRPLQSTEFSETPSPDSDSVNSVEGHSEPSWFKDIKFDDSDTEQIAEEGDDNLANSASPSKRTSVSSFQSTVDSDSAASISLNVELDNVNFHIKKPSKYPHVPPHPADQKEYLVSDTGGQQLSISDAFIKESLFNRRVEEKSKELPFTPLGWHHNNLELLREENEEKQAMERLLSANHNHMMALLQQLLQNESLSSSWRDIIVSLVCQVVQTVRPDVKHQDDDMDIRQFVHIKKIPGGKKFDSVVVNGFVCTKNIAHKKMNSCIKNPKILLLKCSIEYLYREETKFTCIDPIVLQEREFLKNYVQRIVDVRPTLVLVEKTVSRIAQDMLLEHGITLVINVKSQVLERISRMTQGDLVVSMDQLLTKPHLGTCHKFYMQIFQLPNEQTKTLMFFEGCPQHLGCTIKLRGGSDYELARVKEILIFMICVAYHSQLEISFLMDEFAMPPTLMQSPSFHLLTEGRGEEGASQEQVSGSSLPQDPECPREALSSDDSTLLESRTVLEKGELDNKSIPQAVASLKHQDYTTPTCPAGIPCALFALVPESLLPLHMDQQDAVGNEQPETSQQTDEQQDPKSQMKAFRDPLQDDTGMYVTEEVTSSEDQRKTYALTFKQELKDVILCISPVITFREPFLLTEKGMRCSTRDYFPEQIYWSPLLNKEVMEMESRRKKQLLRDLSGLQGMNGSVQAKSIQVLPSHELVSTRIAEHLGDSQTLGRMLADYRARGGRIQSKHLDPFVHSKDASCTSGGKPGNKTESDEERGLIPSDVIWPAKVDCLNPANHQRLCVLFSSSSAQSSNAPSACVSPWIVTMEFYGKNDLTLGIFLERYCFRSSYQCPSMFCDTPMVHHIRRFVHGQGCVQIILKELDSPVPGYQHTILTYSWCRICKQVTPVVALSNESWSMSFAKYLELRFYGHQYTRRANAEPCGHSIHHDYHQYFSYNQMVASFSYSPIRLLEVCVPLPKIFIKRQAPLKVSLLQDLKDFFQKVSQVYLAVDERLASLKTDTFSKTREEKMEDIFAQKEMEEGEFKNWTEKMQARLMSSSVDTPQQLQSIFESLIAKKQSLCEVLQAWNSRLQDLFQQEKGRKRPSVPPSPGRLRQGEESKINAMDTSPRNISPGLHNGEKEDRFLTTLSSQSSTSSTHLQLPTPPEALAEQVVGGPTDLDSASGSEDVFDGHLLGSTDSQVKEKSTMKAIFANLLPGNSYNPIPFPFDPDKHYLMYEHERVPIAVCEKEPSSIIAFALSALDNRPKSSSPIRVPEISGGQTNRTGEAEPQPTKKASGMLSFFRGTAGKSPDLSSQKRETLRGADSAYYQVGQAGKEGLESQGLEPQDEVDGGDTQKKQLTNPHVELQFSDANAKFYCRLYYAGEFHKMREVILGSSEEDFIRSLSHSSPWQARGGKSGAAFYATEDDRFILKQMPRLEVQSFLDFAPHYFNYITNAVQQKRPTALAKILGVYRIGYKNSQNNTEKKLDLLVMENLFYGRKMAQVFDLKGSLRNRNVKTDTGKESCDVVLLDENLLKMVRDNPLYIRSHSKSVLRTSIHSDAHFLSSHLIIDYSLLVGRDDTSNELVVGIIDYIRTFTWDKKLEMVVKSTGILGGQGKMPTVVSPELYRTRFCEAMDKYFLMVPDHWTGLDLNC

Target ORF information:

RefSeq Version	XM_029476086.1
Organism	Mus caroli(Ryukyu mouse)
Definition	Mus caroli phosphoinositide kinase, FYVE-type zinc finger containing (Pikfyve), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_029476086.1

ORF Insert Sequence:

ATGGCCACAG ATGACAAGAG TTCCCCGACA CTGGACTCTG CTAATGATTT GCCTCGCTCT 
CCCGCCAGTC CTTCTCACCT CACTCACTTT AAACCCTTGA CTCCTGACCA GGATGAGCCC 
CCCTTCAAGT CAGCATATAG TTCTTTTGTA AACCTTTTTC GCTTTAACAA AGAGCGGGGA 
GAAGGGGGCC AAGGAGAGCA GCAGTCTCCG AGTTCAAGTT GGGCCAGCCC TCAGATCCCT 
TCAAGAACAC AGTCTGTGAG GTCGCCTGTA CCTTATAAAA AACAGCTTAA TGAGGAGCTC 
CACCGGCGCT CTTCAGTGTT AGCAGAGAAC ACTTTGCCAC ATCCTCAGGA GAGCACAGAC 
TCCAGAAGGA AAGCAGAACC AGCCTGTGGA GGTCATGACC CACGTACAGC TGTTCAGCTT 
CGAAGCCTCA GCACAGTATT GAAACGCCTC AAAGAAATCA TGGAAGGAAA AAGCCAGGAC 
AGTGACCTGA AGCAATATTG GATGCCAGAT AGCCAGTGTA AAGAGTGCTA TGACTGCAGT 
GAAAAGTTTA CAACATTTAG GCGCAGACAC CATTGCAGAC TGTGTGGGCA GATTTTCTGC 
AGTCGTTGTT GTAATCAAGA AATCCCTGGA AAATTTATGG GCTATACAGG AGACCTCCGA 
GCATGCACCT ACTGTAGGAA AATAGCCTTA AGTTATGCTC ATTCCACAGA CAGTAATTCC 
ATTGGGGAAG ACTTGAATGC TCTTTCAGAT TCAACTTGCT CTGTGTCTAT ACTTGATCCA 
AGCGAACCTC GGACACCTGT TGGGAGTAGA AAAGCCAGTC GTAACATATT CTTAGAGGAT 
GATTTAGCCT GGCAAAGTTT GATTCATCCG GATTCCTCAA ATAGTGCTCT TTCAACAAGA 
CTCGTATCTG TTCAAGAGGA TGCTGGGAAG TCTCCTGCTC GGAACAGATC AGCCAGCATT 
ACTAATCTGT CACTGGATCG GTCTGGTTCT CCTATGGTTC CTTCATATGA GACATCTGTC 
AGTCCCCAGG CTAACCGAAA CTACATTAGG ACCGAGACGA CTGAGGATGA ACGCAAAATT 
CTTCTGGACA GTGCTCAGTT AAAGGATCTG TGGAAGAAAA TCTGCCATCA CACCAGTGGG 
ATGGAATTTC AAGATCACCG TTACTGGTTG AGAACACATC CCAACTGCAT TGTAGGAAAG 
GAATTAGTCA ACTGGCTAAT CAGAAATGGA CACATCGCTA CAAGGGCACA AGCTATAGCA 
ATTGGACAAG CAATGGTTGA TGGACGTTGG TTGGATTGTG TTAGTCATCA TGACCAGCTT 
TTCAGGGACG AATATGCGTT GTATAGACCA CTTCAGAGTA CAGAATTTTC TGAGACACCT 
TCTCCAGACA GTGACTCCGT GAACTCTGTG GAAGGACACT CCGAGCCATC CTGGTTTAAA 
GACATAAAAT TTGATGACAG TGACACAGAA CAGATTGCTG AAGAAGGTGA CGATAATTTG 
GCTAATTCTG CCAGTCCTAG CAAGCGCACC TCAGTCAGCA GTTTCCAGTC CACAGTGGAC 
AGTGACTCAG CCGCTTCTAT CAGCCTGAAC GTGGAGCTGG ACAACGTGAA CTTCCATATC 
AAGAAGCCCT CCAAGTACCC ACATGTGCCC CCTCACCCTG CTGACCAAAA AGAGTATTTG 
GTTTCTGACA CTGGAGGACA GCAGCTCTCA ATAAGTGATG CCTTCATCAA AGAGTCCTTA 
TTTAATCGAC GAGTAGAGGA AAAATCCAAA GAGCTGCCTT TTACCCCTTT GGGCTGGCAT 
CATAACAACC TGGAACTCTT GCGAGAGGAG AATGAGGAGA AGCAAGCCAT GGAAAGGCTG 
CTTTCAGCTA ATCATAACCA CATGATGGCC CTACTCCAGC AATTGCTTCA AAACGAGTCA 
TTGTCATCGT CTTGGAGGGA CATCATTGTG TCACTAGTCT GCCAGGTTGT TCAGACAGTC 
CGACCTGACG TCAAGCACCA GGATGATGAC ATGGATATCC GTCAGTTTGT CCATATCAAG 
AAGATCCCAG GTGGAAAGAA ATTTGATTCT GTGGTTGTCA ATGGCTTTGT TTGTACCAAG 
AACATTGCAC ATAAAAAGAT GAATTCCTGT ATTAAAAACC CCAAAATCCT TCTGTTGAAG 
TGTTCTATTG AGTATCTCTA TAGAGAAGAA ACTAAGTTTA CCTGCATTGA TCCTATTGTG 
CTTCAGGAAA GGGAATTCTT GAAGAATTAT GTTCAACGAA TAGTTGATGT TCGACCCACA 
TTGGTTCTTG TTGAGAAAAC CGTGTCTCGG ATTGCTCAGG ACATGTTACT GGAACATGGC 
ATTACTCTGG TCATTAATGT AAAGTCACAA GTTTTAGAAA GAATCAGTCG AATGACCCAA 
GGTGATTTAG TGGTGTCCAT GGACCAGCTG CTCACCAAAC CCCACTTGGG CACTTGCCAC 
AAATTTTATA TGCAGATATT TCAGCTGCCT AATGAACAAA CCAAAACACT GATGTTTTTT 
GAAGGCTGTC CACAGCACCT AGGCTGCACA ATCAAGCTCA GAGGAGGCTC TGATTATGAG 
CTGGCTCGAG TTAAGGAGAT CCTAATATTT ATGATCTGTG TAGCTTATCA TTCTCAGCTA 
GAAATCTCTT TTCTCATGGA TGAGTTCGCT ATGCCTCCAA CGTTAATGCA AAGCCCTTCG 
TTCCATCTTC TGACGGAGGG ACGGGGTGAA GAGGGAGCCT CTCAGGAGCA GGTCAGTGGC 
AGCTCCCTTC CTCAGGATCC TGAGTGCCCT CGTGAGGCCC TGTCTTCTGA CGATAGCACT 
TTGTTGGAAT CAAGGACTGT GCTAGAGAAG GGTGAACTAG ACAATAAAAG TATTCCACAA 
GCTGTTGCCT CTTTGAAGCA TCAAGATTAT ACCACCCCCA CTTGCCCAGC AGGTATCCCC 
TGTGCTCTCT TTGCATTGGT ACCAGAGTCA TTGTTGCCTC TCCATATGGA TCAACAGGAT 
GCTGTAGGAA ATGAACAACC AGAGACATCA CAACAAACAG ATGAGCAACA GGATCCCAAA 
AGCCAGATGA AAGCTTTTAG AGACCCTCTA CAGGATGACA CCGGAATGTA TGTTACTGAG 
GAAGTCACCT CCTCTGAAGA TCAACGAAAG ACTTATGCCT TGACATTTAA ACAGGAATTA 
AAAGACGTAA TCCTCTGTAT CTCTCCAGTT ATTACATTCC GTGAACCTTT CCTTTTAACT 
GAAAAGGGGA TGAGATGCTC AACTCGAGAT TATTTTCCAG AGCAGATTTA CTGGTCTCCT 
CTTCTCAACA AAGAGGTGAT GGAAATGGAG AGCAGGAGGA AGAAACAGCT GCTCAGGGAT 
CTCTCTGGAC TTCAGGGCAT GAATGGCAGT GTTCAGGCCA AGTCTATTCA AGTCTTACCC 
TCACATGAAC TAGTGAGCAC CAGGATTGCT GAACATCTGG GTGACAGCCA GACCTTGGGT 
AGAATGCTAG CTGATTATCG AGCTAGAGGA GGAAGAATTC AGTCAAAACA TTTGGACCCT 
TTTGTCCATT CAAAAGATGC ATCATGTACT TCAGGTGGAA AACCAGGAAA CAAAACTGAG 
AGTGATGAAG AGAGGGGATT GATCCCAAGT GATGTAATAT GGCCAGCAAA GGTGGACTGT 
CTGAATCCTG CTAACCACCA GAGGCTCTGT GTGCTCTTCA GCAGCTCTTC TGCCCAGTCC 
AGCAATGCTC CCAGTGCTTG TGTCAGTCCT TGGATTGTAA CAATGGAGTT TTATGGAAAG 
AATGACCTTA CACTGGGAAT ATTTTTAGAA AGATACTGTT TCAGGTCTTC TTACCAGTGT 
CCGAGCATGT TCTGTGACAC CCCCATGGTT CATCACATTC GACGCTTTGT TCATGGCCAA 
GGCTGTGTAC AGATAATTCT GAAGGAGTTG GATTCTCCAG TGCCTGGATA TCAACATACA 
ATTCTCACAT ACTCCTGGTG CAGAATCTGC AAACAAGTAA CACCAGTTGT TGCTCTTTCA 
AATGAATCCT GGTCTATGTC ATTTGCAAAG TACCTTGAAC TTCGATTTTA TGGCCACCAG 
TACACACGCA GAGCCAACGC TGAGCCCTGC GGTCACTCTA TCCACCATGA TTATCACCAG 
TATTTCTCTT ATAACCAGAT GGTGGCATCT TTCAGTTACT CTCCTATTCG GCTTCTTGAA 
GTATGTGTTC CACTACCAAA AATATTCATT AAGCGTCAAG CCCCACTGAA GGTATCTCTT 
CTTCAGGACC TCAAGGACTT TTTTCAGAAG GTTTCACAGG TGTACCTAGC TGTTGATGAG 
AGACTTGCAT CCTTGAAAAC GGATACATTT AGCAAAACCA GAGAGGAAAA GATGGAAGAT 
ATCTTTGCAC AAAAGGAGAT GGAAGAGGGT GAGTTTAAGA ACTGGACAGA GAAGATGCAA 
GCAAGGCTCA TGTCTTCCTC TGTGGATACC CCTCAGCAAC TGCAGTCCAT TTTTGAGTCA 
CTGATTGCCA AGAAGCAAAG CCTCTGTGAG GTGCTCCAGG CGTGGAACAG CAGGTTGCAG 
GACCTCTTCC AGCAGGAAAA AGGTAGAAAG AGGCCTTCAG TTCCTCCCAG TCCTGGGAGA 
CTGAGACAAG GTGAAGAAAG CAAGATAAAT GCAATGGACA CATCTCCAAG GAATATTTCT 
CCAGGACTTC ACAATGGAGA AAAAGAGGAT CGCTTCTTGA CAACCCTGTC CAGCCAGAGC 
TCTACGAGCT CCACCCACCT CCAGCTGCCC ACTCCTCCCG AGGCCCTGGC CGAGCAGGTA 
GTGGGAGGGC CGACTGATCT GGATTCAGCC AGTGGTTCTG AAGATGTATT TGATGGTCAT 
TTGCTGGGAT CCACAGACAG CCAGGTGAAG GAAAAGTCAA CCATGAAAGC CATCTTTGCT 
AATTTGCTTC CAGGAAACAG CTACAATCCC ATTCCATTTC CTTTTGATCC AGATAAACAC 
TACTTAATGT ATGAACATGA ACGGGTGCCC ATTGCTGTCT GTGAGAAAGA GCCCAGCTCC 
ATCATTGCTT TTGCACTCAG TGCTTTAGAT AACAGACCTA AGAGTAGCAG CCCTATTAGA 
GTACCTGAAA TCAGTGGAGG ACAGACAAAC CGCACAGGAG AAGCAGAACC TCAGCCAACC 
AAAAAAGCTT CAGGCATGTT GTCCTTCTTC AGAGGAACAG CAGGGAAGAG TCCTGACCTG 
TCTTCTCAGA AGAGGGAGAC CTTACGTGGG GCAGACAGTG CTTACTACCA GGTTGGGCAG 
GCTGGCAAGG AGGGGTTGGA GAGTCAAGGC CTGGAGCCTC AAGATGAAGT AGACGGAGGA 
GATACACAGA AGAAACAACT CACAAATCCT CATGTGGAAC TTCAATTTTC TGATGCTAAT 
GCCAAGTTTT ACTGTCGGCT GTACTACGCG GGAGAGTTCC ATAAGATGCG TGAAGTGATT 
CTGGGCAGCA GCGAGGAGGA TTTCATTCGC TCCCTTTCTC ACTCATCTCC CTGGCAGGCC 
CGGGGAGGCA AGTCAGGAGC TGCTTTCTAT GCCACCGAAG ATGATAGATT CATTCTGAAG 
CAAATGCCTC GTTTGGAAGT CCAGTCTTTC CTTGACTTTG CACCACACTA CTTCAATTAT 
ATCACAAATG CTGTTCAACA AAAGAGGCCC ACCGCCTTGG CTAAAATTCT TGGAGTTTAC 
AGAATTGGCT ATAAGAACTC TCAGAACAAC ACTGAGAAGA AGTTAGATCT CCTTGTTATG 
GAAAATCTTT TCTATGGGAG AAAGATGGCA CAGGTTTTTG ATTTGAAGGG TTCACTTAGG 
AATCGAAATG TAAAAACTGA CACTGGGAAA GAGAGCTGTG ATGTGGTTCT GTTGGATGAA 
AACCTCCTAA AGATGGTTCG AGACAACCCT CTCTATATTC GTTCCCATTC CAAATCTGTG 
CTGAGAACCT CCATCCACAG CGACGCCCAT TTCCTTTCCA GCCACCTCAT TATAGACTAT 
TCTCTGCTGG TTGGGCGAGA TGACACTAGC AATGAGCTTG TGGTTGGCAT CATAGATTAC 
ATTCGAACAT TTACATGGGA CAAAAAACTT GAGATGGTTG TGAAGTCAAC AGGAATTTTA 
GGAGGACAAG GTAAAATGCC AACTGTGGTC TCTCCAGAGT TGTATAGGAC TAGATTTTGT 
GAAGCAATGG ACAAGTATTT CTTGATGGTG CCAGACCACT GGACAGGGTT GGATCTGAAT 
TGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Pikfyve ( XM_029476091.1 ) cDNA ORF clone, Mus caroli(Ryukyu mouse) -> XP_029331951.1 Mus caroli phosphoinositide kinase, FYVE-type zinc finger containing (Pikfyve), transcript variant X7, mRNA.

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CloneID	OMv66198
Clone ID Related Accession (Same CDS sequence)	XM_029476091.1
Accession Version	XM_029476091.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6162bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-06-09
Organism	Mus caroli(Ryukyu mouse)
Product	1-phosphatidylinositol 3-phosphate 5-kinase isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_034570.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus caroli Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCACAG ATGACAAGAG TTCCCCGACA CTGGACTCTG CTAATGATTT GCCTCGCTCT 
CCCGCCAGTC CTTCTCACCT CACTCACTTT AAACCCTTGA CTCCTGACCA GGATGAGCCC 
CCCTTCAAGT CAGCATATAG TTCTTTTGTA AACCTTTTTC GCTTTAACAA AGAGCGGGGA 
GAAGGGGGCC AAGGAGAGCA GCAGTCTCCG AGTTCAAGTT GGGCCAGCCC TCAGATCCCT 
TCAAGAACAC AGTCTGTGAG GTCGCCTGTA CCTTATAAAA AACAGCTTAA TGAGGAGCTC 
CACCGGCGCT CTTCAGTGTT AGCAGAGAAC ACTTTGCCAC ATCCTCAGGA GAGCACAGAC 
TCCAGAAGGA AAGCAGAACC AGCCTGTGGA GGTCATGACC CACGTACAGC TGTTCAGCTT 
CGAAGCCTCA GCACAGTATT GAAACGCCTC AAAGAAATCA TGGAAGGAAA AAGCCAGGAC 
AGTGACCTGA AGCAATATTG GATGCCAGAT AGCCAGTGTA AAGAGTGCTA TGACTGCAGT 
GAAAAGTTTA CAACATTTAG GCGCAGACAC CATTGCAGAC TGTGTGGGCA GATTTTCTGC 
AGTCGTTGTT GTAATCAAGA AATCCCTGGA AAATTTATGG GCTATACAGG AGACCTCCGA 
GCATGCACCT ACTGTAGGAA AATAGCCTTA AGTTATGCTC ATTCCACAGA CAGTAATTCC 
ATTGGGGAAG ACTTGAATGC TCTTTCAGAT TCAACTTGCT CTGTGTCTAT ACTTGATCCA 
AGCGAACCTC GGACACCTGT TGGGAGTAGA AAAGCCAGTC GTAACATATT CTTAGAGGAT 
GATTTAGCCT GGCAAAGTTT GATTCATCCG GATTCCTCAA ATAGTGCTCT TTCAACAAGA 
CTCGTATCTG TTCAAGAGGA TGCTGGGAAG TCTCCTGCTC GGAACAGATC AGCCAGCATT 
ACTAATCTGT CACTGGATCG GTCTGGTTCT CCTATGGTTC CTTCATATGA GACATCTGTC 
AGTCCCCAGG CTAACCGAAA CTACATTAGG ACCGAGACGA CTGAGGATGA ACGCAAAATT 
CTTCTGGACA GTGCTCAGTT AAAGGATCTG TGGAAGAAAA TCTGCCATCA CACCAGTGGG 
ATGGAATTTC AAGATCACCG TTACTGGTTG AGAACACATC CCAACTGCAT TGTAGGAAAG 
GAATTAGTCA ACTGGCTAAT CAGAAATGGA CACATCGCTA CAAGGGCACA AGCTATAGCA 
ATTGGACAAG CAATGGTTGA TGGACGTTGG TTGGATTGTG TTAGTCATCA TGACCAGCTT 
TTCAGGGACG AATATGCGTT GTATAGACCA CTTCAGAGTA CAGAATTTTC TGAGACACCT 
TCTCCAGACA GTGACTCCGT GAACTCTGTG GAAGGACACT CCGAGCCATC CTGGTTTAAA 
GACATAAAAT TTGATGACAG TGACACAGAA CAGATTGCTG AAGAAGGTGA CGATAATTTG 
GCTAAGTATT TGGTTTCTGA CACTGGAGGA CAGCAGCTCT CAATAAGTGA TGCCTTCATC 
AAAGAGTCCT TATTTAATCG ACGAGTAGAG GAAAAATCCA AAGAGCTGCC TTTTACCCCT 
TTGGGCTGGC ATCATAACAA CCTGGAACTC TTGCGAGAGG AGAATGAGGA GAAGCAAGCC 
ATGGAAAGGC TGCTTTCAGC TAATCATAAC CACATGATGG CCCTACTCCA GCAATTGCTT 
CAAAACGAGT CATTGTCATC GTCTTGGAGG GACATCATTG TGTCACTAGT CTGCCAGGTT 
GTTCAGACAG TCCGACCTGA CGTCAAGCAC CAGGATGATG ACATGGATAT CCGTCAGTTT 
GTCCATATCA AGAAGATCCC AGGTGGAAAG AAATTTGATT CTGTGGTTGT CAATGGCTTT 
GTTTGTACCA AGAACATTGC ACATAAAAAG ATGAATTCCT GTATTAAAAA CCCCAAAATC 
CTTCTGTTGA AGTGTTCTAT TGAGTATCTC TATAGAGAAG AAACTAAGTT TACCTGCATT 
GATCCTATTG TGCTTCAGGA AAGGGAATTC TTGAAGAATT ATGTTCAACG AATAGTTGAT 
GTTCGACCCA CATTGGTTCT TGTTGAGAAA ACCGTGTCTC GGATTGCTCA GGACATGTTA 
CTGGAACATG GCATTACTCT GGTCATTAAT GTAAAGTCAC AAGTTTTAGA AAGAATCAGT 
CGAATGACCC AAGGTGATTT AGTGGTGTCC ATGGACCAGC TGCTCACCAA ACCCCACTTG 
GGCACTTGCC ACAAATTTTA TATGCAGATA TTTCAGCTGC CTAATGAACA AACCAAAACA 
CTGATGTTTT TTGAAGGCTG TCCACAGCAC CTAGGCTGCA CAATCAAGCT CAGAGGAGGC 
TCTGATTATG AGCTGGCTCG AGTTAAGGAG ATCCTAATAT TTATGATCTG TGTAGCTTAT 
CATTCTCAGC TAGAAATCTC TTTTCTCATG GATGAGTTCG CTATGCCTCC AACGTTAATG 
CAAAGCCCTT CGTTCCATCT TCTGACGGAG GGACGGGGTG AAGAGGGAGC CTCTCAGGAG 
CAGGTCAGTG GCAGCTCCCT TCCTCAGGAT CCTGAGTGCC CTCGTGAGGC CCTGTCTTCT 
GACGATAGCA CTTTGTTGGA ATCAAGGACT GTGCTAGAGA AGGGTGAACT AGACAATAAA 
AGTATTCCAC AAGCTGTTGC CTCTTTGAAG CATCAAGATT ATACCACCCC CACTTGCCCA 
GCAGGTATCC CCTGTGCTCT CTTTGCATTG GTACCAGAGT CATTGTTGCC TCTCCATATG 
GATCAACAGG ATGCTGTAGG AAATGAACAA CCAGAGACAT CACAACAAAC AGATGAGCAA 
CAGGATCCCA AAAGCCAGAT GAAAGCTTTT AGAGACCCTC TACAGGATGA CACCGGAATG 
TATGTTACTG AGGAAGTCAC CTCCTCTGAA GATCAACGAA AGACTTATGC CTTGACATTT 
AAACAGGAAT TAAAAGACGT AATCCTCTGT ATCTCTCCAG TTATTACATT CCGTGAACCT 
TTCCTTTTAA CTGAAAAGGG GATGAGATGC TCAACTCGAG ATTATTTTCC AGAGCAGATT 
TACTGGTCTC CTCTTCTCAA CAAAGAGGTG ATGGAAATGG AGAGCAGGAG GAAGAAACAG 
CTGCTCAGGG ATCTCTCTGG ACTTCAGGGC ATGAATGGCA GTGTTCAGGC CAAGTCTATT 
CAAGTCTTAC CCTCACATGA ACTAGTGAGC ACCAGGATTG CTGAACATCT GGGTGACAGC 
CAGACCTTGG GTAGAATGCT AGCTGATTAT CGAGCTAGAG GAGGAAGAAT TCAGTCAAAA 
CATTTGGACC CTTTTGTCCA TTCAAAAGAT GCATCATGTA CTTCAGGTGG AAAACCAGGA 
AACAAAACTG AGAGTGATGA AGAGAGGGGA TTGATCCCAA GTGATGTAAT ATGGCCAGCA 
AAGGTGGACT GTCTGAATCC TGCTAACCAC CAGAGGCTCT GTGTGCTCTT CAGCAGCTCT 
TCTGCCCAGT CCAGCAATGC TCCCAGTGCT TGTGTCAGTC CTTGGATTGT AACAATGGAG 
TTTTATGGAA AGAATGACCT TACACTGGGA ATATTTTTAG AAAGATACTG TTTCAGGTCT 
TCTTACCAGT GTCCGAGCAT GTTCTGTGAC ACCCCCATGG TTCATCACAT TCGACGCTTT 
GTTCATGGCC AAGGCTGTGT ACAGATAATT CTGAAGGAGT TGGATTCTCC AGTGCCTGGA 
TATCAACATA CAATTCTCAC ATACTCCTGG TGCAGAATCT GCAAACAAGT AACACCAGTT 
GTTGCTCTTT CAAATGAATC CTGGTCTATG TCATTTGCAA AGTACCTTGA ACTTCGATTT 
TATGGCCACC AGTACACACG CAGAGCCAAC GCTGAGCCCT GCGGTCACTC TATCCACCAT 
GATTATCACC AGTATTTCTC TTATAACCAG ATGGTGGCAT CTTTCAGTTA CTCTCCTATT 
CGGCTTCTTG AAGTATGTGT TCCACTACCA AAAATATTCA TTAAGCGTCA AGCCCCACTG 
AAGGTATCTC TTCTTCAGGA CCTCAAGGAC TTTTTTCAGA AGGTTTCACA GGTGTACCTA 
GCTGTTGATG AGAGACTTGC ATCCTTGAAA ACGGATACAT TTAGCAAAAC CAGAGAGGAA 
AAGATGGAAG ATATCTTTGC ACAAAAGGAG ATGGAAGAGG GTGAGTTTAA GAACTGGACA 
GAGAAGATGC AAGCAAGGCT CATGTCTTCC TCTGTGGATA CCCCTCAGCA ACTGCAGTCC 
ATTTTTGAGT CACTGATTGC CAAGAAGCAA AGCCTCTGTG AGGTGCTCCA GGCGTGGAAC 
AGCAGGTTGC AGGACCTCTT CCAGCAGGAA AAAGGTAGAA AGAGGCCTTC AGTTCCTCCC 
AGTCCTGGGA GACTGAGACA AGGTGAAGAA AGCAAGATAA ATGCAATGGA CACATCTCCA 
AGGAATATTT CTCCAGGACT TCACAATGGA GAAAAAGAGG ATCGCTTCTT GACAACCCTG 
TCCAGCCAGA GCTCTACGAG CTCCACCCAC CTCCAGCTGC CCACTCCTCC CGAGGCCCTG 
GCCGAGCAGG TAGTGGGAGG GCCGACTGAT CTGGATTCAG CCAGTGGTTC TGAAGATGTA 
TTTGATGGTC ATTTGCTGGG ATCCACAGAC AGCCAGGTGA AGGAAAAGTC AACCATGAAA 
GCCATCTTTG CTAATTTGCT TCCAGGAAAC AGCTACAATC CCATTCCATT TCCTTTTGAT 
CCAGATAAAC ACTACTTAAT GTATGAACAT GAACGGGTGC CCATTGCTGT CTGTGAGAAA 
GAGCCCAGCT CCATCATTGC TTTTGCACTC AGTTGTAAAG AATACCGAAA TGCCTTAGAG 
GAATTGTCCA AAGCGACTCT GTGGAACAGT GCTGAAGAAG GGCTTCCAGC CAATAGTGCT 
TTAGATAACA GACCTAAGAG TAGCAGCCCT ATTAGAGTAC CTGAAATCAG TGGAGGACAG 
ACAAACCGCA CAGGAGAAGC AGAACCTCAG CCAACCAAAA AAGCTTCAGG CATGTTGTCC 
TTCTTCAGAG GAACAGCAGG GAAGAGTCCT GACCTGTCTT CTCAGAAGAG GGAGACCTTA 
CGTGGGGCAG ACAGTGCTTA CTACCAGGTT GGGCAGGCTG GCAAGGAGGG GTTGGAGAGT 
CAAGGCCTGG AGCCTCAAGA TGAAGTAGAC GGAGGAGATA CACAGAAGAA ACAACTCACA 
AATCCTCATG TGGAACTTCA ATTTTCTGAT GCTAATGCCA AGTTTTACTG TCGGCTGTAC 
TACGCGGGAG AGTTCCATAA GATGCGTGAA GTGATTCTGG GCAGCAGCGA GGAGGATTTC 
ATTCGCTCCC TTTCTCACTC ATCTCCCTGG CAGGCCCGGG GAGGCAAGTC AGGAGCTGCT 
TTCTATGCCA CCGAAGATGA TAGATTCATT CTGAAGCAAA TGCCTCGTTT GGAAGTCCAG 
TCTTTCCTTG ACTTTGCACC ACACTACTTC AATTATATCA CAAATGCTGT TCAACAAAAG 
AGGCCCACCG CCTTGGCTAA AATTCTTGGA GTTTACAGAA TTGGCTATAA GAACTCTCAG 
AACAACACTG AGAAGAAGTT AGATCTCCTT GTTATGGAAA ATCTTTTCTA TGGGAGAAAG 
ATGGCACAGG TTTTTGATTT GAAGGGTTCA CTTAGGAATC GAAATGTAAA AACTGACACT 
GGGAAAGAGA GCTGTGATGT GGTTCTGTTG GATGAAAACC TCCTAAAGAT GGTTCGAGAC 
AACCCTCTCT ATATTCGTTC CCATTCCAAA TCTGTGCTGA GAACCTCCAT CCACAGCGAC 
GCCCATTTCC TTTCCAGCCA CCTCATTATA GACTATTCTC TGCTGGTTGG GCGAGATGAC 
ACTAGCAATG AGCTTGTGGT TGGCATCATA GATTACATTC GAACATTTAC ATGGGACAAA 
AAACTTGAGA TGGTTGTGAA GTCAACAGGA ATTTTAGGAG GACAAGGTAA AATGCCAACT 
GTGGTCTCTC CAGAGTTGTA TAGGACTAGA TTTTGTGAAG CAATGGACAA GTATTTCTTG 
ATGGTGCCAG ACCACTGGAC AGGGTTGGAT CTGAATTGCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_029331951.1
CDS	164..6325
Translation	MATDDKSSPTLDSANDLPRSPASPSHLTHFKPLTPDQDEPPFKSAYSSFVNLFRFNKERGEGGQGEQQSPSSSWASPQIPSRTQSVRSPVPYKKQLNEELHRRSSVLAENTLPHPQESTDSRRKAEPACGGHDPRTAVQLRSLSTVLKRLKEIMEGKSQDSDLKQYWMPDSQCKECYDCSEKFTTFRRRHHCRLCGQIFCSRCCNQEIPGKFMGYTGDLRACTYCRKIALSYAHSTDSNSIGEDLNALSDSTCSVSILDPSEPRTPVGSRKASRNIFLEDDLAWQSLIHPDSSNSALSTRLVSVQEDAGKSPARNRSASITNLSLDRSGSPMVPSYETSVSPQANRNYIRTETTEDERKILLDSAQLKDLWKKICHHTSGMEFQDHRYWLRTHPNCIVGKELVNWLIRNGHIATRAQAIAIGQAMVDGRWLDCVSHHDQLFRDEYALYRPLQSTEFSETPSPDSDSVNSVEGHSEPSWFKDIKFDDSDTEQIAEEGDDNLAKYLVSDTGGQQLSISDAFIKESLFNRRVEEKSKELPFTPLGWHHNNLELLREENEEKQAMERLLSANHNHMMALLQQLLQNESLSSSWRDIIVSLVCQVVQTVRPDVKHQDDDMDIRQFVHIKKIPGGKKFDSVVVNGFVCTKNIAHKKMNSCIKNPKILLLKCSIEYLYREETKFTCIDPIVLQEREFLKNYVQRIVDVRPTLVLVEKTVSRIAQDMLLEHGITLVINVKSQVLERISRMTQGDLVVSMDQLLTKPHLGTCHKFYMQIFQLPNEQTKTLMFFEGCPQHLGCTIKLRGGSDYELARVKEILIFMICVAYHSQLEISFLMDEFAMPPTLMQSPSFHLLTEGRGEEGASQEQVSGSSLPQDPECPREALSSDDSTLLESRTVLEKGELDNKSIPQAVASLKHQDYTTPTCPAGIPCALFALVPESLLPLHMDQQDAVGNEQPETSQQTDEQQDPKSQMKAFRDPLQDDTGMYVTEEVTSSEDQRKTYALTFKQELKDVILCISPVITFREPFLLTEKGMRCSTRDYFPEQIYWSPLLNKEVMEMESRRKKQLLRDLSGLQGMNGSVQAKSIQVLPSHELVSTRIAEHLGDSQTLGRMLADYRARGGRIQSKHLDPFVHSKDASCTSGGKPGNKTESDEERGLIPSDVIWPAKVDCLNPANHQRLCVLFSSSSAQSSNAPSACVSPWIVTMEFYGKNDLTLGIFLERYCFRSSYQCPSMFCDTPMVHHIRRFVHGQGCVQIILKELDSPVPGYQHTILTYSWCRICKQVTPVVALSNESWSMSFAKYLELRFYGHQYTRRANAEPCGHSIHHDYHQYFSYNQMVASFSYSPIRLLEVCVPLPKIFIKRQAPLKVSLLQDLKDFFQKVSQVYLAVDERLASLKTDTFSKTREEKMEDIFAQKEMEEGEFKNWTEKMQARLMSSSVDTPQQLQSIFESLIAKKQSLCEVLQAWNSRLQDLFQQEKGRKRPSVPPSPGRLRQGEESKINAMDTSPRNISPGLHNGEKEDRFLTTLSSQSSTSSTHLQLPTPPEALAEQVVGGPTDLDSASGSEDVFDGHLLGSTDSQVKEKSTMKAIFANLLPGNSYNPIPFPFDPDKHYLMYEHERVPIAVCEKEPSSIIAFALSCKEYRNALEELSKATLWNSAEEGLPANSALDNRPKSSSPIRVPEISGGQTNRTGEAEPQPTKKASGMLSFFRGTAGKSPDLSSQKRETLRGADSAYYQVGQAGKEGLESQGLEPQDEVDGGDTQKKQLTNPHVELQFSDANAKFYCRLYYAGEFHKMREVILGSSEEDFIRSLSHSSPWQARGGKSGAAFYATEDDRFILKQMPRLEVQSFLDFAPHYFNYITNAVQQKRPTALAKILGVYRIGYKNSQNNTEKKLDLLVMENLFYGRKMAQVFDLKGSLRNRNVKTDTGKESCDVVLLDENLLKMVRDNPLYIRSHSKSVLRTSIHSDAHFLSSHLIIDYSLLVGRDDTSNELVVGIIDYIRTFTWDKKLEMVVKSTGILGGQGKMPTVVSPELYRTRFCEAMDKYFLMVPDHWTGLDLNC

Target ORF information:

RefSeq Version	XM_029476091.1
Organism	Mus caroli(Ryukyu mouse)
Definition	Mus caroli phosphoinositide kinase, FYVE-type zinc finger containing (Pikfyve), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_029476091.1

ORF Insert Sequence:

ATGGCCACAG ATGACAAGAG TTCCCCGACA CTGGACTCTG CTAATGATTT GCCTCGCTCT 
CCCGCCAGTC CTTCTCACCT CACTCACTTT AAACCCTTGA CTCCTGACCA GGATGAGCCC 
CCCTTCAAGT CAGCATATAG TTCTTTTGTA AACCTTTTTC GCTTTAACAA AGAGCGGGGA 
GAAGGGGGCC AAGGAGAGCA GCAGTCTCCG AGTTCAAGTT GGGCCAGCCC TCAGATCCCT 
TCAAGAACAC AGTCTGTGAG GTCGCCTGTA CCTTATAAAA AACAGCTTAA TGAGGAGCTC 
CACCGGCGCT CTTCAGTGTT AGCAGAGAAC ACTTTGCCAC ATCCTCAGGA GAGCACAGAC 
TCCAGAAGGA AAGCAGAACC AGCCTGTGGA GGTCATGACC CACGTACAGC TGTTCAGCTT 
CGAAGCCTCA GCACAGTATT GAAACGCCTC AAAGAAATCA TGGAAGGAAA AAGCCAGGAC 
AGTGACCTGA AGCAATATTG GATGCCAGAT AGCCAGTGTA AAGAGTGCTA TGACTGCAGT 
GAAAAGTTTA CAACATTTAG GCGCAGACAC CATTGCAGAC TGTGTGGGCA GATTTTCTGC 
AGTCGTTGTT GTAATCAAGA AATCCCTGGA AAATTTATGG GCTATACAGG AGACCTCCGA 
GCATGCACCT ACTGTAGGAA AATAGCCTTA AGTTATGCTC ATTCCACAGA CAGTAATTCC 
ATTGGGGAAG ACTTGAATGC TCTTTCAGAT TCAACTTGCT CTGTGTCTAT ACTTGATCCA 
AGCGAACCTC GGACACCTGT TGGGAGTAGA AAAGCCAGTC GTAACATATT CTTAGAGGAT 
GATTTAGCCT GGCAAAGTTT GATTCATCCG GATTCCTCAA ATAGTGCTCT TTCAACAAGA 
CTCGTATCTG TTCAAGAGGA TGCTGGGAAG TCTCCTGCTC GGAACAGATC AGCCAGCATT 
ACTAATCTGT CACTGGATCG GTCTGGTTCT CCTATGGTTC CTTCATATGA GACATCTGTC 
AGTCCCCAGG CTAACCGAAA CTACATTAGG ACCGAGACGA CTGAGGATGA ACGCAAAATT 
CTTCTGGACA GTGCTCAGTT AAAGGATCTG TGGAAGAAAA TCTGCCATCA CACCAGTGGG 
ATGGAATTTC AAGATCACCG TTACTGGTTG AGAACACATC CCAACTGCAT TGTAGGAAAG 
GAATTAGTCA ACTGGCTAAT CAGAAATGGA CACATCGCTA CAAGGGCACA AGCTATAGCA 
ATTGGACAAG CAATGGTTGA TGGACGTTGG TTGGATTGTG TTAGTCATCA TGACCAGCTT 
TTCAGGGACG AATATGCGTT GTATAGACCA CTTCAGAGTA CAGAATTTTC TGAGACACCT 
TCTCCAGACA GTGACTCCGT GAACTCTGTG GAAGGACACT CCGAGCCATC CTGGTTTAAA 
GACATAAAAT TTGATGACAG TGACACAGAA CAGATTGCTG AAGAAGGTGA CGATAATTTG 
GCTAAGTATT TGGTTTCTGA CACTGGAGGA CAGCAGCTCT CAATAAGTGA TGCCTTCATC 
AAAGAGTCCT TATTTAATCG ACGAGTAGAG GAAAAATCCA AAGAGCTGCC TTTTACCCCT 
TTGGGCTGGC ATCATAACAA CCTGGAACTC TTGCGAGAGG AGAATGAGGA GAAGCAAGCC 
ATGGAAAGGC TGCTTTCAGC TAATCATAAC CACATGATGG CCCTACTCCA GCAATTGCTT 
CAAAACGAGT CATTGTCATC GTCTTGGAGG GACATCATTG TGTCACTAGT CTGCCAGGTT 
GTTCAGACAG TCCGACCTGA CGTCAAGCAC CAGGATGATG ACATGGATAT CCGTCAGTTT 
GTCCATATCA AGAAGATCCC AGGTGGAAAG AAATTTGATT CTGTGGTTGT CAATGGCTTT 
GTTTGTACCA AGAACATTGC ACATAAAAAG ATGAATTCCT GTATTAAAAA CCCCAAAATC 
CTTCTGTTGA AGTGTTCTAT TGAGTATCTC TATAGAGAAG AAACTAAGTT TACCTGCATT 
GATCCTATTG TGCTTCAGGA AAGGGAATTC TTGAAGAATT ATGTTCAACG AATAGTTGAT 
GTTCGACCCA CATTGGTTCT TGTTGAGAAA ACCGTGTCTC GGATTGCTCA GGACATGTTA 
CTGGAACATG GCATTACTCT GGTCATTAAT GTAAAGTCAC AAGTTTTAGA AAGAATCAGT 
CGAATGACCC AAGGTGATTT AGTGGTGTCC ATGGACCAGC TGCTCACCAA ACCCCACTTG 
GGCACTTGCC ACAAATTTTA TATGCAGATA TTTCAGCTGC CTAATGAACA AACCAAAACA 
CTGATGTTTT TTGAAGGCTG TCCACAGCAC CTAGGCTGCA CAATCAAGCT CAGAGGAGGC 
TCTGATTATG AGCTGGCTCG AGTTAAGGAG ATCCTAATAT TTATGATCTG TGTAGCTTAT 
CATTCTCAGC TAGAAATCTC TTTTCTCATG GATGAGTTCG CTATGCCTCC AACGTTAATG 
CAAAGCCCTT CGTTCCATCT TCTGACGGAG GGACGGGGTG AAGAGGGAGC CTCTCAGGAG 
CAGGTCAGTG GCAGCTCCCT TCCTCAGGAT CCTGAGTGCC CTCGTGAGGC CCTGTCTTCT 
GACGATAGCA CTTTGTTGGA ATCAAGGACT GTGCTAGAGA AGGGTGAACT AGACAATAAA 
AGTATTCCAC AAGCTGTTGC CTCTTTGAAG CATCAAGATT ATACCACCCC CACTTGCCCA 
GCAGGTATCC CCTGTGCTCT CTTTGCATTG GTACCAGAGT CATTGTTGCC TCTCCATATG 
GATCAACAGG ATGCTGTAGG AAATGAACAA CCAGAGACAT CACAACAAAC AGATGAGCAA 
CAGGATCCCA AAAGCCAGAT GAAAGCTTTT AGAGACCCTC TACAGGATGA CACCGGAATG 
TATGTTACTG AGGAAGTCAC CTCCTCTGAA GATCAACGAA AGACTTATGC CTTGACATTT 
AAACAGGAAT TAAAAGACGT AATCCTCTGT ATCTCTCCAG TTATTACATT CCGTGAACCT 
TTCCTTTTAA CTGAAAAGGG GATGAGATGC TCAACTCGAG ATTATTTTCC AGAGCAGATT 
TACTGGTCTC CTCTTCTCAA CAAAGAGGTG ATGGAAATGG AGAGCAGGAG GAAGAAACAG 
CTGCTCAGGG ATCTCTCTGG ACTTCAGGGC ATGAATGGCA GTGTTCAGGC CAAGTCTATT 
CAAGTCTTAC CCTCACATGA ACTAGTGAGC ACCAGGATTG CTGAACATCT GGGTGACAGC 
CAGACCTTGG GTAGAATGCT AGCTGATTAT CGAGCTAGAG GAGGAAGAAT TCAGTCAAAA 
CATTTGGACC CTTTTGTCCA TTCAAAAGAT GCATCATGTA CTTCAGGTGG AAAACCAGGA 
AACAAAACTG AGAGTGATGA AGAGAGGGGA TTGATCCCAA GTGATGTAAT ATGGCCAGCA 
AAGGTGGACT GTCTGAATCC TGCTAACCAC CAGAGGCTCT GTGTGCTCTT CAGCAGCTCT 
TCTGCCCAGT CCAGCAATGC TCCCAGTGCT TGTGTCAGTC CTTGGATTGT AACAATGGAG 
TTTTATGGAA AGAATGACCT TACACTGGGA ATATTTTTAG AAAGATACTG TTTCAGGTCT 
TCTTACCAGT GTCCGAGCAT GTTCTGTGAC ACCCCCATGG TTCATCACAT TCGACGCTTT 
GTTCATGGCC AAGGCTGTGT ACAGATAATT CTGAAGGAGT TGGATTCTCC AGTGCCTGGA 
TATCAACATA CAATTCTCAC ATACTCCTGG TGCAGAATCT GCAAACAAGT AACACCAGTT 
GTTGCTCTTT CAAATGAATC CTGGTCTATG TCATTTGCAA AGTACCTTGA ACTTCGATTT 
TATGGCCACC AGTACACACG CAGAGCCAAC GCTGAGCCCT GCGGTCACTC TATCCACCAT 
GATTATCACC AGTATTTCTC TTATAACCAG ATGGTGGCAT CTTTCAGTTA CTCTCCTATT 
CGGCTTCTTG AAGTATGTGT TCCACTACCA AAAATATTCA TTAAGCGTCA AGCCCCACTG 
AAGGTATCTC TTCTTCAGGA CCTCAAGGAC TTTTTTCAGA AGGTTTCACA GGTGTACCTA 
GCTGTTGATG AGAGACTTGC ATCCTTGAAA ACGGATACAT TTAGCAAAAC CAGAGAGGAA 
AAGATGGAAG ATATCTTTGC ACAAAAGGAG ATGGAAGAGG GTGAGTTTAA GAACTGGACA 
GAGAAGATGC AAGCAAGGCT CATGTCTTCC TCTGTGGATA CCCCTCAGCA ACTGCAGTCC 
ATTTTTGAGT CACTGATTGC CAAGAAGCAA AGCCTCTGTG AGGTGCTCCA GGCGTGGAAC 
AGCAGGTTGC AGGACCTCTT CCAGCAGGAA AAAGGTAGAA AGAGGCCTTC AGTTCCTCCC 
AGTCCTGGGA GACTGAGACA AGGTGAAGAA AGCAAGATAA ATGCAATGGA CACATCTCCA 
AGGAATATTT CTCCAGGACT TCACAATGGA GAAAAAGAGG ATCGCTTCTT GACAACCCTG 
TCCAGCCAGA GCTCTACGAG CTCCACCCAC CTCCAGCTGC CCACTCCTCC CGAGGCCCTG 
GCCGAGCAGG TAGTGGGAGG GCCGACTGAT CTGGATTCAG CCAGTGGTTC TGAAGATGTA 
TTTGATGGTC ATTTGCTGGG ATCCACAGAC AGCCAGGTGA AGGAAAAGTC AACCATGAAA 
GCCATCTTTG CTAATTTGCT TCCAGGAAAC AGCTACAATC CCATTCCATT TCCTTTTGAT 
CCAGATAAAC ACTACTTAAT GTATGAACAT GAACGGGTGC CCATTGCTGT CTGTGAGAAA 
GAGCCCAGCT CCATCATTGC TTTTGCACTC AGTTGTAAAG AATACCGAAA TGCCTTAGAG 
GAATTGTCCA AAGCGACTCT GTGGAACAGT GCTGAAGAAG GGCTTCCAGC CAATAGTGCT 
TTAGATAACA GACCTAAGAG TAGCAGCCCT ATTAGAGTAC CTGAAATCAG TGGAGGACAG 
ACAAACCGCA CAGGAGAAGC AGAACCTCAG CCAACCAAAA AAGCTTCAGG CATGTTGTCC 
TTCTTCAGAG GAACAGCAGG GAAGAGTCCT GACCTGTCTT CTCAGAAGAG GGAGACCTTA 
CGTGGGGCAG ACAGTGCTTA CTACCAGGTT GGGCAGGCTG GCAAGGAGGG GTTGGAGAGT 
CAAGGCCTGG AGCCTCAAGA TGAAGTAGAC GGAGGAGATA CACAGAAGAA ACAACTCACA 
AATCCTCATG TGGAACTTCA ATTTTCTGAT GCTAATGCCA AGTTTTACTG TCGGCTGTAC 
TACGCGGGAG AGTTCCATAA GATGCGTGAA GTGATTCTGG GCAGCAGCGA GGAGGATTTC 
ATTCGCTCCC TTTCTCACTC ATCTCCCTGG CAGGCCCGGG GAGGCAAGTC AGGAGCTGCT 
TTCTATGCCA CCGAAGATGA TAGATTCATT CTGAAGCAAA TGCCTCGTTT GGAAGTCCAG 
TCTTTCCTTG ACTTTGCACC ACACTACTTC AATTATATCA CAAATGCTGT TCAACAAAAG 
AGGCCCACCG CCTTGGCTAA AATTCTTGGA GTTTACAGAA TTGGCTATAA GAACTCTCAG 
AACAACACTG AGAAGAAGTT AGATCTCCTT GTTATGGAAA ATCTTTTCTA TGGGAGAAAG 
ATGGCACAGG TTTTTGATTT GAAGGGTTCA CTTAGGAATC GAAATGTAAA AACTGACACT 
GGGAAAGAGA GCTGTGATGT GGTTCTGTTG GATGAAAACC TCCTAAAGAT GGTTCGAGAC 
AACCCTCTCT ATATTCGTTC CCATTCCAAA TCTGTGCTGA GAACCTCCAT CCACAGCGAC 
GCCCATTTCC TTTCCAGCCA CCTCATTATA GACTATTCTC TGCTGGTTGG GCGAGATGAC 
ACTAGCAATG AGCTTGTGGT TGGCATCATA GATTACATTC GAACATTTAC ATGGGACAAA 
AAACTTGAGA TGGTTGTGAA GTCAACAGGA ATTTTAGGAG GACAAGGTAA AATGCCAACT 
GTGGTCTCTC CAGAGTTGTA TAGGACTAGA TTTTGTGAAG CAATGGACAA GTATTTCTTG 
ATGGTGCCAG ACCACTGGAC AGGGTTGGAT CTGAATTGCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Pikfyve ( XM_029476096.1 ) cDNA ORF clone, Mus caroli(Ryukyu mouse) -> XP_029331956.1 Mus caroli phosphoinositide kinase, FYVE-type zinc finger containing (Pikfyve), transcript variant X8, mRNA.

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CloneID	OMv66199
Clone ID Related Accession (Same CDS sequence)	XM_029476096.1
Accession Version	XM_029476096.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6159bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-06-09
Organism	Mus caroli(Ryukyu mouse)
Product	1-phosphatidylinositol 3-phosphate 5-kinase isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_034570.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus caroli Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCACAG ATGACAAGAG TTCCCCGACA CTGGACTCTG CTAATGATTT GCCTCGCTCT 
CCCGCCAGTC CTTCTCACCT CACTCACTTT AAACCCTTGA CTCCTGACCA GGATGAGCCC 
CCCTTCAAGT CAGCATATAG TTCTTTTGTA AACCTTTTTC GCTTTAACAA AGAGCGGGGA 
GAAGGGGGCC AAGGAGAGCA GCAGTCTCCG AGTTCAAGTT GGGCCAGCCC TCAGATCCCT 
TCAAGAACAC AGTCTGTGAG GTCGCCTGTA CCTTATAAAA AACAGCTTAA TGAGGAGCTC 
CACCGGCGCT CTTCAGTGTT AGAGAACACT TTGCCACATC CTCAGGAGAG CACAGACTCC 
AGAAGGAAAG CAGAACCAGC CTGTGGAGGT CATGACCCAC GTACAGCTGT TCAGCTTCGA 
AGCCTCAGCA CAGTATTGAA ACGCCTCAAA GAAATCATGG AAGGAAAAAG CCAGGACAGT 
GACCTGAAGC AATATTGGAT GCCAGATAGC CAGTGTAAAG AGTGCTATGA CTGCAGTGAA 
AAGTTTACAA CATTTAGGCG CAGACACCAT TGCAGACTGT GTGGGCAGAT TTTCTGCAGT 
CGTTGTTGTA ATCAAGAAAT CCCTGGAAAA TTTATGGGCT ATACAGGAGA CCTCCGAGCA 
TGCACCTACT GTAGGAAAAT AGCCTTAAGT TATGCTCATT CCACAGACAG TAATTCCATT 
GGGGAAGACT TGAATGCTCT TTCAGATTCA ACTTGCTCTG TGTCTATACT TGATCCAAGC 
GAACCTCGGA CACCTGTTGG GAGTAGAAAA GCCAGTCGTA ACATATTCTT AGAGGATGAT 
TTAGCCTGGC AAAGTTTGAT TCATCCGGAT TCCTCAAATA GTGCTCTTTC AACAAGACTC 
GTATCTGTTC AAGAGGATGC TGGGAAGTCT CCTGCTCGGA ACAGATCAGC CAGCATTACT 
AATCTGTCAC TGGATCGGTC TGGTTCTCCT ATGGTTCCTT CATATGAGAC ATCTGTCAGT 
CCCCAGGCTA ACCGAAACTA CATTAGGACC GAGACGACTG AGGATGAACG CAAAATTCTT 
CTGGACAGTG CTCAGTTAAA GGATCTGTGG AAGAAAATCT GCCATCACAC CAGTGGGATG 
GAATTTCAAG ATCACCGTTA CTGGTTGAGA ACACATCCCA ACTGCATTGT AGGAAAGGAA 
TTAGTCAACT GGCTAATCAG AAATGGACAC ATCGCTACAA GGGCACAAGC TATAGCAATT 
GGACAAGCAA TGGTTGATGG ACGTTGGTTG GATTGTGTTA GTCATCATGA CCAGCTTTTC 
AGGGACGAAT ATGCGTTGTA TAGACCACTT CAGAGTACAG AATTTTCTGA GACACCTTCT 
CCAGACAGTG ACTCCGTGAA CTCTGTGGAA GGACACTCCG AGCCATCCTG GTTTAAAGAC 
ATAAAATTTG ATGACAGTGA CACAGAACAG ATTGCTGAAG AAGGTGACGA TAATTTGGCT 
AAGTATTTGG TTTCTGACAC TGGAGGACAG CAGCTCTCAA TAAGTGATGC CTTCATCAAA 
GAGTCCTTAT TTAATCGACG AGTAGAGGAA AAATCCAAAG AGCTGCCTTT TACCCCTTTG 
GGCTGGCATC ATAACAACCT GGAACTCTTG CGAGAGGAGA ATGAGGAGAA GCAAGCCATG 
GAAAGGCTGC TTTCAGCTAA TCATAACCAC ATGATGGCCC TACTCCAGCA ATTGCTTCAA 
AACGAGTCAT TGTCATCGTC TTGGAGGGAC ATCATTGTGT CACTAGTCTG CCAGGTTGTT 
CAGACAGTCC GACCTGACGT CAAGCACCAG GATGATGACA TGGATATCCG TCAGTTTGTC 
CATATCAAGA AGATCCCAGG TGGAAAGAAA TTTGATTCTG TGGTTGTCAA TGGCTTTGTT 
TGTACCAAGA ACATTGCACA TAAAAAGATG AATTCCTGTA TTAAAAACCC CAAAATCCTT 
CTGTTGAAGT GTTCTATTGA GTATCTCTAT AGAGAAGAAA CTAAGTTTAC CTGCATTGAT 
CCTATTGTGC TTCAGGAAAG GGAATTCTTG AAGAATTATG TTCAACGAAT AGTTGATGTT 
CGACCCACAT TGGTTCTTGT TGAGAAAACC GTGTCTCGGA TTGCTCAGGA CATGTTACTG 
GAACATGGCA TTACTCTGGT CATTAATGTA AAGTCACAAG TTTTAGAAAG AATCAGTCGA 
ATGACCCAAG GTGATTTAGT GGTGTCCATG GACCAGCTGC TCACCAAACC CCACTTGGGC 
ACTTGCCACA AATTTTATAT GCAGATATTT CAGCTGCCTA ATGAACAAAC CAAAACACTG 
ATGTTTTTTG AAGGCTGTCC ACAGCACCTA GGCTGCACAA TCAAGCTCAG AGGAGGCTCT 
GATTATGAGC TGGCTCGAGT TAAGGAGATC CTAATATTTA TGATCTGTGT AGCTTATCAT 
TCTCAGCTAG AAATCTCTTT TCTCATGGAT GAGTTCGCTA TGCCTCCAAC GTTAATGCAA 
AGCCCTTCGT TCCATCTTCT GACGGAGGGA CGGGGTGAAG AGGGAGCCTC TCAGGAGCAG 
GTCAGTGGCA GCTCCCTTCC TCAGGATCCT GAGTGCCCTC GTGAGGCCCT GTCTTCTGAC 
GATAGCACTT TGTTGGAATC AAGGACTGTG CTAGAGAAGG GTGAACTAGA CAATAAAAGT 
ATTCCACAAG CTGTTGCCTC TTTGAAGCAT CAAGATTATA CCACCCCCAC TTGCCCAGCA 
GGTATCCCCT GTGCTCTCTT TGCATTGGTA CCAGAGTCAT TGTTGCCTCT CCATATGGAT 
CAACAGGATG CTGTAGGAAA TGAACAACCA GAGACATCAC AACAAACAGA TGAGCAACAG 
GATCCCAAAA GCCAGATGAA AGCTTTTAGA GACCCTCTAC AGGATGACAC CGGAATGTAT 
GTTACTGAGG AAGTCACCTC CTCTGAAGAT CAACGAAAGA CTTATGCCTT GACATTTAAA 
CAGGAATTAA AAGACGTAAT CCTCTGTATC TCTCCAGTTA TTACATTCCG TGAACCTTTC 
CTTTTAACTG AAAAGGGGAT GAGATGCTCA ACTCGAGATT ATTTTCCAGA GCAGATTTAC 
TGGTCTCCTC TTCTCAACAA AGAGGTGATG GAAATGGAGA GCAGGAGGAA GAAACAGCTG 
CTCAGGGATC TCTCTGGACT TCAGGGCATG AATGGCAGTG TTCAGGCCAA GTCTATTCAA 
GTCTTACCCT CACATGAACT AGTGAGCACC AGGATTGCTG AACATCTGGG TGACAGCCAG 
ACCTTGGGTA GAATGCTAGC TGATTATCGA GCTAGAGGAG GAAGAATTCA GTCAAAACAT 
TTGGACCCTT TTGTCCATTC AAAAGATGCA TCATGTACTT CAGGTGGAAA ACCAGGAAAC 
AAAACTGAGA GTGATGAAGA GAGGGGATTG ATCCCAAGTG ATGTAATATG GCCAGCAAAG 
GTGGACTGTC TGAATCCTGC TAACCACCAG AGGCTCTGTG TGCTCTTCAG CAGCTCTTCT 
GCCCAGTCCA GCAATGCTCC CAGTGCTTGT GTCAGTCCTT GGATTGTAAC AATGGAGTTT 
TATGGAAAGA ATGACCTTAC ACTGGGAATA TTTTTAGAAA GATACTGTTT CAGGTCTTCT 
TACCAGTGTC CGAGCATGTT CTGTGACACC CCCATGGTTC ATCACATTCG ACGCTTTGTT 
CATGGCCAAG GCTGTGTACA GATAATTCTG AAGGAGTTGG ATTCTCCAGT GCCTGGATAT 
CAACATACAA TTCTCACATA CTCCTGGTGC AGAATCTGCA AACAAGTAAC ACCAGTTGTT 
GCTCTTTCAA ATGAATCCTG GTCTATGTCA TTTGCAAAGT ACCTTGAACT TCGATTTTAT 
GGCCACCAGT ACACACGCAG AGCCAACGCT GAGCCCTGCG GTCACTCTAT CCACCATGAT 
TATCACCAGT ATTTCTCTTA TAACCAGATG GTGGCATCTT TCAGTTACTC TCCTATTCGG 
CTTCTTGAAG TATGTGTTCC ACTACCAAAA ATATTCATTA AGCGTCAAGC CCCACTGAAG 
GTATCTCTTC TTCAGGACCT CAAGGACTTT TTTCAGAAGG TTTCACAGGT GTACCTAGCT 
GTTGATGAGA GACTTGCATC CTTGAAAACG GATACATTTA GCAAAACCAG AGAGGAAAAG 
ATGGAAGATA TCTTTGCACA AAAGGAGATG GAAGAGGGTG AGTTTAAGAA CTGGACAGAG 
AAGATGCAAG CAAGGCTCAT GTCTTCCTCT GTGGATACCC CTCAGCAACT GCAGTCCATT 
TTTGAGTCAC TGATTGCCAA GAAGCAAAGC CTCTGTGAGG TGCTCCAGGC GTGGAACAGC 
AGGTTGCAGG ACCTCTTCCA GCAGGAAAAA GGTAGAAAGA GGCCTTCAGT TCCTCCCAGT 
CCTGGGAGAC TGAGACAAGG TGAAGAAAGC AAGATAAATG CAATGGACAC ATCTCCAAGG 
AATATTTCTC CAGGACTTCA CAATGGAGAA AAAGAGGATC GCTTCTTGAC AACCCTGTCC 
AGCCAGAGCT CTACGAGCTC CACCCACCTC CAGCTGCCCA CTCCTCCCGA GGCCCTGGCC 
GAGCAGGTAG TGGGAGGGCC GACTGATCTG GATTCAGCCA GTGGTTCTGA AGATGTATTT 
GATGGTCATT TGCTGGGATC CACAGACAGC CAGGTGAAGG AAAAGTCAAC CATGAAAGCC 
ATCTTTGCTA ATTTGCTTCC AGGAAACAGC TACAATCCCA TTCCATTTCC TTTTGATCCA 
GATAAACACT ACTTAATGTA TGAACATGAA CGGGTGCCCA TTGCTGTCTG TGAGAAAGAG 
CCCAGCTCCA TCATTGCTTT TGCACTCAGT TGTAAAGAAT ACCGAAATGC CTTAGAGGAA 
TTGTCCAAAG CGACTCTGTG GAACAGTGCT GAAGAAGGGC TTCCAGCCAA TAGTGCTTTA 
GATAACAGAC CTAAGAGTAG CAGCCCTATT AGAGTACCTG AAATCAGTGG AGGACAGACA 
AACCGCACAG GAGAAGCAGA ACCTCAGCCA ACCAAAAAAG CTTCAGGCAT GTTGTCCTTC 
TTCAGAGGAA CAGCAGGGAA GAGTCCTGAC CTGTCTTCTC AGAAGAGGGA GACCTTACGT 
GGGGCAGACA GTGCTTACTA CCAGGTTGGG CAGGCTGGCA AGGAGGGGTT GGAGAGTCAA 
GGCCTGGAGC CTCAAGATGA AGTAGACGGA GGAGATACAC AGAAGAAACA ACTCACAAAT 
CCTCATGTGG AACTTCAATT TTCTGATGCT AATGCCAAGT TTTACTGTCG GCTGTACTAC 
GCGGGAGAGT TCCATAAGAT GCGTGAAGTG ATTCTGGGCA GCAGCGAGGA GGATTTCATT 
CGCTCCCTTT CTCACTCATC TCCCTGGCAG GCCCGGGGAG GCAAGTCAGG AGCTGCTTTC 
TATGCCACCG AAGATGATAG ATTCATTCTG AAGCAAATGC CTCGTTTGGA AGTCCAGTCT 
TTCCTTGACT TTGCACCACA CTACTTCAAT TATATCACAA ATGCTGTTCA ACAAAAGAGG 
CCCACCGCCT TGGCTAAAAT TCTTGGAGTT TACAGAATTG GCTATAAGAA CTCTCAGAAC 
AACACTGAGA AGAAGTTAGA TCTCCTTGTT ATGGAAAATC TTTTCTATGG GAGAAAGATG 
GCACAGGTTT TTGATTTGAA GGGTTCACTT AGGAATCGAA ATGTAAAAAC TGACACTGGG 
AAAGAGAGCT GTGATGTGGT TCTGTTGGAT GAAAACCTCC TAAAGATGGT TCGAGACAAC 
CCTCTCTATA TTCGTTCCCA TTCCAAATCT GTGCTGAGAA CCTCCATCCA CAGCGACGCC 
CATTTCCTTT CCAGCCACCT CATTATAGAC TATTCTCTGC TGGTTGGGCG AGATGACACT 
AGCAATGAGC TTGTGGTTGG CATCATAGAT TACATTCGAA CATTTACATG GGACAAAAAA 
CTTGAGATGG TTGTGAAGTC AACAGGAATT TTAGGAGGAC AAGGTAAAAT GCCAACTGTG 
GTCTCTCCAG AGTTGTATAG GACTAGATTT TGTGAAGCAA TGGACAAGTA TTTCTTGATG 
GTGCCAGACC ACTGGACAGG GTTGGATCTG AATTGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_029331956.1
CDS	164..6322
Translation	MATDDKSSPTLDSANDLPRSPASPSHLTHFKPLTPDQDEPPFKSAYSSFVNLFRFNKERGEGGQGEQQSPSSSWASPQIPSRTQSVRSPVPYKKQLNEELHRRSSVLENTLPHPQESTDSRRKAEPACGGHDPRTAVQLRSLSTVLKRLKEIMEGKSQDSDLKQYWMPDSQCKECYDCSEKFTTFRRRHHCRLCGQIFCSRCCNQEIPGKFMGYTGDLRACTYCRKIALSYAHSTDSNSIGEDLNALSDSTCSVSILDPSEPRTPVGSRKASRNIFLEDDLAWQSLIHPDSSNSALSTRLVSVQEDAGKSPARNRSASITNLSLDRSGSPMVPSYETSVSPQANRNYIRTETTEDERKILLDSAQLKDLWKKICHHTSGMEFQDHRYWLRTHPNCIVGKELVNWLIRNGHIATRAQAIAIGQAMVDGRWLDCVSHHDQLFRDEYALYRPLQSTEFSETPSPDSDSVNSVEGHSEPSWFKDIKFDDSDTEQIAEEGDDNLAKYLVSDTGGQQLSISDAFIKESLFNRRVEEKSKELPFTPLGWHHNNLELLREENEEKQAMERLLSANHNHMMALLQQLLQNESLSSSWRDIIVSLVCQVVQTVRPDVKHQDDDMDIRQFVHIKKIPGGKKFDSVVVNGFVCTKNIAHKKMNSCIKNPKILLLKCSIEYLYREETKFTCIDPIVLQEREFLKNYVQRIVDVRPTLVLVEKTVSRIAQDMLLEHGITLVINVKSQVLERISRMTQGDLVVSMDQLLTKPHLGTCHKFYMQIFQLPNEQTKTLMFFEGCPQHLGCTIKLRGGSDYELARVKEILIFMICVAYHSQLEISFLMDEFAMPPTLMQSPSFHLLTEGRGEEGASQEQVSGSSLPQDPECPREALSSDDSTLLESRTVLEKGELDNKSIPQAVASLKHQDYTTPTCPAGIPCALFALVPESLLPLHMDQQDAVGNEQPETSQQTDEQQDPKSQMKAFRDPLQDDTGMYVTEEVTSSEDQRKTYALTFKQELKDVILCISPVITFREPFLLTEKGMRCSTRDYFPEQIYWSPLLNKEVMEMESRRKKQLLRDLSGLQGMNGSVQAKSIQVLPSHELVSTRIAEHLGDSQTLGRMLADYRARGGRIQSKHLDPFVHSKDASCTSGGKPGNKTESDEERGLIPSDVIWPAKVDCLNPANHQRLCVLFSSSSAQSSNAPSACVSPWIVTMEFYGKNDLTLGIFLERYCFRSSYQCPSMFCDTPMVHHIRRFVHGQGCVQIILKELDSPVPGYQHTILTYSWCRICKQVTPVVALSNESWSMSFAKYLELRFYGHQYTRRANAEPCGHSIHHDYHQYFSYNQMVASFSYSPIRLLEVCVPLPKIFIKRQAPLKVSLLQDLKDFFQKVSQVYLAVDERLASLKTDTFSKTREEKMEDIFAQKEMEEGEFKNWTEKMQARLMSSSVDTPQQLQSIFESLIAKKQSLCEVLQAWNSRLQDLFQQEKGRKRPSVPPSPGRLRQGEESKINAMDTSPRNISPGLHNGEKEDRFLTTLSSQSSTSSTHLQLPTPPEALAEQVVGGPTDLDSASGSEDVFDGHLLGSTDSQVKEKSTMKAIFANLLPGNSYNPIPFPFDPDKHYLMYEHERVPIAVCEKEPSSIIAFALSCKEYRNALEELSKATLWNSAEEGLPANSALDNRPKSSSPIRVPEISGGQTNRTGEAEPQPTKKASGMLSFFRGTAGKSPDLSSQKRETLRGADSAYYQVGQAGKEGLESQGLEPQDEVDGGDTQKKQLTNPHVELQFSDANAKFYCRLYYAGEFHKMREVILGSSEEDFIRSLSHSSPWQARGGKSGAAFYATEDDRFILKQMPRLEVQSFLDFAPHYFNYITNAVQQKRPTALAKILGVYRIGYKNSQNNTEKKLDLLVMENLFYGRKMAQVFDLKGSLRNRNVKTDTGKESCDVVLLDENLLKMVRDNPLYIRSHSKSVLRTSIHSDAHFLSSHLIIDYSLLVGRDDTSNELVVGIIDYIRTFTWDKKLEMVVKSTGILGGQGKMPTVVSPELYRTRFCEAMDKYFLMVPDHWTGLDLNC

Target ORF information:

RefSeq Version	XM_029476096.1
Organism	Mus caroli(Ryukyu mouse)
Definition	Mus caroli phosphoinositide kinase, FYVE-type zinc finger containing (Pikfyve), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_029476096.1

ORF Insert Sequence:

ATGGCCACAG ATGACAAGAG TTCCCCGACA CTGGACTCTG CTAATGATTT GCCTCGCTCT 
CCCGCCAGTC CTTCTCACCT CACTCACTTT AAACCCTTGA CTCCTGACCA GGATGAGCCC 
CCCTTCAAGT CAGCATATAG TTCTTTTGTA AACCTTTTTC GCTTTAACAA AGAGCGGGGA 
GAAGGGGGCC AAGGAGAGCA GCAGTCTCCG AGTTCAAGTT GGGCCAGCCC TCAGATCCCT 
TCAAGAACAC AGTCTGTGAG GTCGCCTGTA CCTTATAAAA AACAGCTTAA TGAGGAGCTC 
CACCGGCGCT CTTCAGTGTT AGAGAACACT TTGCCACATC CTCAGGAGAG CACAGACTCC 
AGAAGGAAAG CAGAACCAGC CTGTGGAGGT CATGACCCAC GTACAGCTGT TCAGCTTCGA 
AGCCTCAGCA CAGTATTGAA ACGCCTCAAA GAAATCATGG AAGGAAAAAG CCAGGACAGT 
GACCTGAAGC AATATTGGAT GCCAGATAGC CAGTGTAAAG AGTGCTATGA CTGCAGTGAA 
AAGTTTACAA CATTTAGGCG CAGACACCAT TGCAGACTGT GTGGGCAGAT TTTCTGCAGT 
CGTTGTTGTA ATCAAGAAAT CCCTGGAAAA TTTATGGGCT ATACAGGAGA CCTCCGAGCA 
TGCACCTACT GTAGGAAAAT AGCCTTAAGT TATGCTCATT CCACAGACAG TAATTCCATT 
GGGGAAGACT TGAATGCTCT TTCAGATTCA ACTTGCTCTG TGTCTATACT TGATCCAAGC 
GAACCTCGGA CACCTGTTGG GAGTAGAAAA GCCAGTCGTA ACATATTCTT AGAGGATGAT 
TTAGCCTGGC AAAGTTTGAT TCATCCGGAT TCCTCAAATA GTGCTCTTTC AACAAGACTC 
GTATCTGTTC AAGAGGATGC TGGGAAGTCT CCTGCTCGGA ACAGATCAGC CAGCATTACT 
AATCTGTCAC TGGATCGGTC TGGTTCTCCT ATGGTTCCTT CATATGAGAC ATCTGTCAGT 
CCCCAGGCTA ACCGAAACTA CATTAGGACC GAGACGACTG AGGATGAACG CAAAATTCTT 
CTGGACAGTG CTCAGTTAAA GGATCTGTGG AAGAAAATCT GCCATCACAC CAGTGGGATG 
GAATTTCAAG ATCACCGTTA CTGGTTGAGA ACACATCCCA ACTGCATTGT AGGAAAGGAA 
TTAGTCAACT GGCTAATCAG AAATGGACAC ATCGCTACAA GGGCACAAGC TATAGCAATT 
GGACAAGCAA TGGTTGATGG ACGTTGGTTG GATTGTGTTA GTCATCATGA CCAGCTTTTC 
AGGGACGAAT ATGCGTTGTA TAGACCACTT CAGAGTACAG AATTTTCTGA GACACCTTCT 
CCAGACAGTG ACTCCGTGAA CTCTGTGGAA GGACACTCCG AGCCATCCTG GTTTAAAGAC 
ATAAAATTTG ATGACAGTGA CACAGAACAG ATTGCTGAAG AAGGTGACGA TAATTTGGCT 
AAGTATTTGG TTTCTGACAC TGGAGGACAG CAGCTCTCAA TAAGTGATGC CTTCATCAAA 
GAGTCCTTAT TTAATCGACG AGTAGAGGAA AAATCCAAAG AGCTGCCTTT TACCCCTTTG 
GGCTGGCATC ATAACAACCT GGAACTCTTG CGAGAGGAGA ATGAGGAGAA GCAAGCCATG 
GAAAGGCTGC TTTCAGCTAA TCATAACCAC ATGATGGCCC TACTCCAGCA ATTGCTTCAA 
AACGAGTCAT TGTCATCGTC TTGGAGGGAC ATCATTGTGT CACTAGTCTG CCAGGTTGTT 
CAGACAGTCC GACCTGACGT CAAGCACCAG GATGATGACA TGGATATCCG TCAGTTTGTC 
CATATCAAGA AGATCCCAGG TGGAAAGAAA TTTGATTCTG TGGTTGTCAA TGGCTTTGTT 
TGTACCAAGA ACATTGCACA TAAAAAGATG AATTCCTGTA TTAAAAACCC CAAAATCCTT 
CTGTTGAAGT GTTCTATTGA GTATCTCTAT AGAGAAGAAA CTAAGTTTAC CTGCATTGAT 
CCTATTGTGC TTCAGGAAAG GGAATTCTTG AAGAATTATG TTCAACGAAT AGTTGATGTT 
CGACCCACAT TGGTTCTTGT TGAGAAAACC GTGTCTCGGA TTGCTCAGGA CATGTTACTG 
GAACATGGCA TTACTCTGGT CATTAATGTA AAGTCACAAG TTTTAGAAAG AATCAGTCGA 
ATGACCCAAG GTGATTTAGT GGTGTCCATG GACCAGCTGC TCACCAAACC CCACTTGGGC 
ACTTGCCACA AATTTTATAT GCAGATATTT CAGCTGCCTA ATGAACAAAC CAAAACACTG 
ATGTTTTTTG AAGGCTGTCC ACAGCACCTA GGCTGCACAA TCAAGCTCAG AGGAGGCTCT 
GATTATGAGC TGGCTCGAGT TAAGGAGATC CTAATATTTA TGATCTGTGT AGCTTATCAT 
TCTCAGCTAG AAATCTCTTT TCTCATGGAT GAGTTCGCTA TGCCTCCAAC GTTAATGCAA 
AGCCCTTCGT TCCATCTTCT GACGGAGGGA CGGGGTGAAG AGGGAGCCTC TCAGGAGCAG 
GTCAGTGGCA GCTCCCTTCC TCAGGATCCT GAGTGCCCTC GTGAGGCCCT GTCTTCTGAC 
GATAGCACTT TGTTGGAATC AAGGACTGTG CTAGAGAAGG GTGAACTAGA CAATAAAAGT 
ATTCCACAAG CTGTTGCCTC TTTGAAGCAT CAAGATTATA CCACCCCCAC TTGCCCAGCA 
GGTATCCCCT GTGCTCTCTT TGCATTGGTA CCAGAGTCAT TGTTGCCTCT CCATATGGAT 
CAACAGGATG CTGTAGGAAA TGAACAACCA GAGACATCAC AACAAACAGA TGAGCAACAG 
GATCCCAAAA GCCAGATGAA AGCTTTTAGA GACCCTCTAC AGGATGACAC CGGAATGTAT 
GTTACTGAGG AAGTCACCTC CTCTGAAGAT CAACGAAAGA CTTATGCCTT GACATTTAAA 
CAGGAATTAA AAGACGTAAT CCTCTGTATC TCTCCAGTTA TTACATTCCG TGAACCTTTC 
CTTTTAACTG AAAAGGGGAT GAGATGCTCA ACTCGAGATT ATTTTCCAGA GCAGATTTAC 
TGGTCTCCTC TTCTCAACAA AGAGGTGATG GAAATGGAGA GCAGGAGGAA GAAACAGCTG 
CTCAGGGATC TCTCTGGACT TCAGGGCATG AATGGCAGTG TTCAGGCCAA GTCTATTCAA 
GTCTTACCCT CACATGAACT AGTGAGCACC AGGATTGCTG AACATCTGGG TGACAGCCAG 
ACCTTGGGTA GAATGCTAGC TGATTATCGA GCTAGAGGAG GAAGAATTCA GTCAAAACAT 
TTGGACCCTT TTGTCCATTC AAAAGATGCA TCATGTACTT CAGGTGGAAA ACCAGGAAAC 
AAAACTGAGA GTGATGAAGA GAGGGGATTG ATCCCAAGTG ATGTAATATG GCCAGCAAAG 
GTGGACTGTC TGAATCCTGC TAACCACCAG AGGCTCTGTG TGCTCTTCAG CAGCTCTTCT 
GCCCAGTCCA GCAATGCTCC CAGTGCTTGT GTCAGTCCTT GGATTGTAAC AATGGAGTTT 
TATGGAAAGA ATGACCTTAC ACTGGGAATA TTTTTAGAAA GATACTGTTT CAGGTCTTCT 
TACCAGTGTC CGAGCATGTT CTGTGACACC CCCATGGTTC ATCACATTCG ACGCTTTGTT 
CATGGCCAAG GCTGTGTACA GATAATTCTG AAGGAGTTGG ATTCTCCAGT GCCTGGATAT 
CAACATACAA TTCTCACATA CTCCTGGTGC AGAATCTGCA AACAAGTAAC ACCAGTTGTT 
GCTCTTTCAA ATGAATCCTG GTCTATGTCA TTTGCAAAGT ACCTTGAACT TCGATTTTAT 
GGCCACCAGT ACACACGCAG AGCCAACGCT GAGCCCTGCG GTCACTCTAT CCACCATGAT 
TATCACCAGT ATTTCTCTTA TAACCAGATG GTGGCATCTT TCAGTTACTC TCCTATTCGG 
CTTCTTGAAG TATGTGTTCC ACTACCAAAA ATATTCATTA AGCGTCAAGC CCCACTGAAG 
GTATCTCTTC TTCAGGACCT CAAGGACTTT TTTCAGAAGG TTTCACAGGT GTACCTAGCT 
GTTGATGAGA GACTTGCATC CTTGAAAACG GATACATTTA GCAAAACCAG AGAGGAAAAG 
ATGGAAGATA TCTTTGCACA AAAGGAGATG GAAGAGGGTG AGTTTAAGAA CTGGACAGAG 
AAGATGCAAG CAAGGCTCAT GTCTTCCTCT GTGGATACCC CTCAGCAACT GCAGTCCATT 
TTTGAGTCAC TGATTGCCAA GAAGCAAAGC CTCTGTGAGG TGCTCCAGGC GTGGAACAGC 
AGGTTGCAGG ACCTCTTCCA GCAGGAAAAA GGTAGAAAGA GGCCTTCAGT TCCTCCCAGT 
CCTGGGAGAC TGAGACAAGG TGAAGAAAGC AAGATAAATG CAATGGACAC ATCTCCAAGG 
AATATTTCTC CAGGACTTCA CAATGGAGAA AAAGAGGATC GCTTCTTGAC AACCCTGTCC 
AGCCAGAGCT CTACGAGCTC CACCCACCTC CAGCTGCCCA CTCCTCCCGA GGCCCTGGCC 
GAGCAGGTAG TGGGAGGGCC GACTGATCTG GATTCAGCCA GTGGTTCTGA AGATGTATTT 
GATGGTCATT TGCTGGGATC CACAGACAGC CAGGTGAAGG AAAAGTCAAC CATGAAAGCC 
ATCTTTGCTA ATTTGCTTCC AGGAAACAGC TACAATCCCA TTCCATTTCC TTTTGATCCA 
GATAAACACT ACTTAATGTA TGAACATGAA CGGGTGCCCA TTGCTGTCTG TGAGAAAGAG 
CCCAGCTCCA TCATTGCTTT TGCACTCAGT TGTAAAGAAT ACCGAAATGC CTTAGAGGAA 
TTGTCCAAAG CGACTCTGTG GAACAGTGCT GAAGAAGGGC TTCCAGCCAA TAGTGCTTTA 
GATAACAGAC CTAAGAGTAG CAGCCCTATT AGAGTACCTG AAATCAGTGG AGGACAGACA 
AACCGCACAG GAGAAGCAGA ACCTCAGCCA ACCAAAAAAG CTTCAGGCAT GTTGTCCTTC 
TTCAGAGGAA CAGCAGGGAA GAGTCCTGAC CTGTCTTCTC AGAAGAGGGA GACCTTACGT 
GGGGCAGACA GTGCTTACTA CCAGGTTGGG CAGGCTGGCA AGGAGGGGTT GGAGAGTCAA 
GGCCTGGAGC CTCAAGATGA AGTAGACGGA GGAGATACAC AGAAGAAACA ACTCACAAAT 
CCTCATGTGG AACTTCAATT TTCTGATGCT AATGCCAAGT TTTACTGTCG GCTGTACTAC 
GCGGGAGAGT TCCATAAGAT GCGTGAAGTG ATTCTGGGCA GCAGCGAGGA GGATTTCATT 
CGCTCCCTTT CTCACTCATC TCCCTGGCAG GCCCGGGGAG GCAAGTCAGG AGCTGCTTTC 
TATGCCACCG AAGATGATAG ATTCATTCTG AAGCAAATGC CTCGTTTGGA AGTCCAGTCT 
TTCCTTGACT TTGCACCACA CTACTTCAAT TATATCACAA ATGCTGTTCA ACAAAAGAGG 
CCCACCGCCT TGGCTAAAAT TCTTGGAGTT TACAGAATTG GCTATAAGAA CTCTCAGAAC 
AACACTGAGA AGAAGTTAGA TCTCCTTGTT ATGGAAAATC TTTTCTATGG GAGAAAGATG 
GCACAGGTTT TTGATTTGAA GGGTTCACTT AGGAATCGAA ATGTAAAAAC TGACACTGGG 
AAAGAGAGCT GTGATGTGGT TCTGTTGGAT GAAAACCTCC TAAAGATGGT TCGAGACAAC 
CCTCTCTATA TTCGTTCCCA TTCCAAATCT GTGCTGAGAA CCTCCATCCA CAGCGACGCC 
CATTTCCTTT CCAGCCACCT CATTATAGAC TATTCTCTGC TGGTTGGGCG AGATGACACT 
AGCAATGAGC TTGTGGTTGG CATCATAGAT TACATTCGAA CATTTACATG GGACAAAAAA 
CTTGAGATGG TTGTGAAGTC AACAGGAATT TTAGGAGGAC AAGGTAAAAT GCCAACTGTG 
GTCTCTCCAG AGTTGTATAG GACTAGATTT TGTGAAGCAA TGGACAAGTA TTTCTTGATG 
GTGCCAGACC ACTGGACAGG GTTGGATCTG AATTGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	Pikfyve
Entrez Gene ID	110293039
Full Name	phosphoinositide kinase, FYVE-type zinc finger containing
Gene Type	protein-coding
Organism	Mus caroli(Ryukyu mouse)

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

Pikfyve cDNA ORF clone, Mus caroli(Ryukyu mouse)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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