Ppef1 cDNA ORF clone, Mus caroli(Ryukyu mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Ppef1
Entrez Gene ID	110286808
Full Name	protein phosphatase with EF-hand domain 1
Gene Type	protein-coding
Organism	Mus caroli(Ryukyu mouse)

mRNA and Protein(s)

mRNA	Protein	Name
XM_021153415.1	XP_021009074.1	serine/threonine-protein phosphatase with EF-hands 1

Pathways from BioSystems
Gene Ontology

The following Ppef1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Ppef1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMv10205	XM_021153415.1 Latest version!	Mus caroli protein phosphatase with EF-hand domain 1 (Ppef1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Ppef1 ( XM_021153415.1 ) cDNA ORF clone, Mus caroli(Ryukyu mouse) -> XP_021009074.1 Mus caroli protein phosphatase with EF-hand domain 1 (Ppef1), mRNA.

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CloneID	OMv10205
Clone ID Related Accession (Same CDS sequence)	XM_021153415.1
Accession Version	XM_021153415.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2013bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-06-09
Organism	Mus caroli(Ryukyu mouse)
Product	serine/threonine-protein phosphatase with EF-hands 1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_034589.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus caroli Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGCTGTG GCACTTCTTC AAAAAAAGGC AACAAATCTA AGAAAGAGAA CGCAGAGCTA 
ATCTCCAGTA TCAACTTGGT GACTTCAGCA TCATTCATAA CGGACCACTC AAGGGACCTA 
ATGATAAAAG CCGCATTAGT CATCCAAAAT TGGTACCGGC GGTACAGAGC TCGACTGAGG 
GCCAGACAAC ATTATGCCCT CGCCATCTTC CAGTCTATTG AATATTCTGA CGAGCAAGGT 
CAAATGCAGC TATCCAGCTT CTTTTCGTTC ATGTTGGAAA ACTATACAAA GACAAATAAT 
GAAGATTCAG CATTAGTAAC TCGAATTTTT GACAACACCA GGGGGGAAAG CCAAATCAAG 
GATAGAGATG AATTTTTGGG GTTGATAGAA GTGCCTGACT CCTATGATGG TCCTCGGCTG 
CAGTTTCCTC TGACGTTTAC TGACATTCAT ATACTTCTTC AGGCCTTCAA GCAACAGCAG 
ATACTTCATG CTCACTACGT CTTAGAGGTG CTATTTGAAG CTAGGAAAGT CCTGAAGCAA 
ATGCCGAATA TCTCTCATGT AAAAACCTTT CCCGCCAAAG AGATAACAAT CTGTGGTGAT 
TTGCATGGGA AACTGGATGA TCTTATGCTT ATTTTCTATA AGAATGGCCT GCCTTCAGAG 
AACAATCCAT ATGTTTTTAA TGGTGACTTT GTAGATCGAG GAAACAATTC CATGGAGATC 
CTAATGATCC TGCTTGTTTG TTTTCTTGTC TACCCCTCTG ACCTGCACCT CAACAGAGGG 
AACCATGAAG ACTTTATGAT GAACCTGAGG TATGGCTTCA CAAAAGAAAT TTTACAAAAA 
TACAAGCTAC ATGGGAGAAA AATCTTACAA GTCTTGGAAG AAGTCTATAC CTGGCTCCCA 
ATCGGTACAA TTATTGACAA CGAAATCCTG GTCATACATG GTGGGATATC AGAATCCACA 
GACTTGAATA CACTCCACCA GTTACAAAGA AATAAAATGA AATCTGTGTT GATGCCGCCA 
GTGTTGATAA ATCAAGAGAC TGGCGTAAAG AAAAATAAAT CAGGTTCAAA TTATGTTGAA 
CCAGACAAGA CTCCTTCTGA AGACTTGACA AAGCAGGAAT GGGAACAAAT AGTTGACATT 
CTGTGGAGTG ACCCCAGAGG CAAAAAAGGC TGTTATCCAA ACACAAGCCG AGGAGGGGGC 
TGCTATTTTG GACCAGATGT TACCTCTAAG GTTCTTAGTA AAAACCAGTT GAAGATGCTC 
ATTAGGTCTC ATGAGTGTAA ACCCGACGGG TATGAAGTCT CTCATGATGG AAAGGTCATT 
ACTGTATTTT CTGCTTCTAA TTATTATGAA GAAGGCAGCA ACCGAGGAGC TTACATCAGA 
CTAAGTTATG GTACAATGCC ACAATTTTTC CAGTACCAAG TAACTAGTAC ATCATGCCTG 
AACCCTCTTC ACCAAAGGAT GAATGCTATG GAATCCAGTG CTTTCAAAAT CCTAAAGGAA 
AAAATGATTT CACGAAAAAC TGATCTCATC AACGCTTTCG AGCTCCGTGA CCACAGTAGG 
TCAGGAAGGA TTTCATTGGC CGAGTGGGCA TTTTCTATGG AGAGCATTTT GGGGCTGAAC 
TTACCATGGA GGTCTCTGAG TTCACACCTG GTAACCATTG ATTCCAGCGG AAGTGTTGAC 
TACATGTCCA GCTTTGACGA TATTCGTATT GAAAAGCCTA TGAAAGATAT GAAGTCCAAT 
CTAACTGAAA CTATGTACAG ATACCGATCT GACCTGAAAA TCATATTTAA TATCATCGAC 
AGTGATCAAT CAGGTCTGAT CTCCATGGAT GAATTTCGGA CCATGTGGAA ACTTTTCAAT 
GCTCATTACA AAGCTCATAT TGATGATTCC CAAATTGATG AGCTTGCCAG CATAGTGGAC 
TTCAACAAAG ATGGCAACAT AGACTTTAAT GAGTTTTTAA AGGCTTTCTA TGTAGTACAC 
AAATATGATA AGCCAGGGAG CAGTCTTGCT TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021009074.1
CDS	348..2360
Translation	MGCGTSSKKGNKSKKENAELISSINLVTSASFITDHSRDLMIKAALVIQNWYRRYRARLRARQHYALAIFQSIEYSDEQGQMQLSSFFSFMLENYTKTNNEDSALVTRIFDNTRGESQIKDRDEFLGLIEVPDSYDGPRLQFPLTFTDIHILLQAFKQQQILHAHYVLEVLFEARKVLKQMPNISHVKTFPAKEITICGDLHGKLDDLMLIFYKNGLPSENNPYVFNGDFVDRGNNSMEILMILLVCFLVYPSDLHLNRGNHEDFMMNLRYGFTKEILQKYKLHGRKILQVLEEVYTWLPIGTIIDNEILVIHGGISESTDLNTLHQLQRNKMKSVLMPPVLINQETGVKKNKSGSNYVEPDKTPSEDLTKQEWEQIVDILWSDPRGKKGCYPNTSRGGGCYFGPDVTSKVLSKNQLKMLIRSHECKPDGYEVSHDGKVITVFSASNYYEEGSNRGAYIRLSYGTMPQFFQYQVTSTSCLNPLHQRMNAMESSAFKILKEKMISRKTDLINAFELRDHSRSGRISLAEWAFSMESILGLNLPWRSLSSHLVTIDSSGSVDYMSSFDDIRIEKPMKDMKSNLTETMYRYRSDLKIIFNIIDSDQSGLISMDEFRTMWKLFNAHYKAHIDDSQIDELASIVDFNKDGNIDFNEFLKAFYVVHKYDKPGSSLA

Target ORF information:

RefSeq Version	XM_021153415.1
Organism	Mus caroli(Ryukyu mouse)
Definition	Mus caroli protein phosphatase with EF-hand domain 1 (Ppef1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021153415.1

ORF Insert Sequence:

ATGGGCTGTG GCACTTCTTC AAAAAAAGGC AACAAATCTA AGAAAGAGAA CGCAGAGCTA 
ATCTCCAGTA TCAACTTGGT GACTTCAGCA TCATTCATAA CGGACCACTC AAGGGACCTA 
ATGATAAAAG CCGCATTAGT CATCCAAAAT TGGTACCGGC GGTACAGAGC TCGACTGAGG 
GCCAGACAAC ATTATGCCCT CGCCATCTTC CAGTCTATTG AATATTCTGA CGAGCAAGGT 
CAAATGCAGC TATCCAGCTT CTTTTCGTTC ATGTTGGAAA ACTATACAAA GACAAATAAT 
GAAGATTCAG CATTAGTAAC TCGAATTTTT GACAACACCA GGGGGGAAAG CCAAATCAAG 
GATAGAGATG AATTTTTGGG GTTGATAGAA GTGCCTGACT CCTATGATGG TCCTCGGCTG 
CAGTTTCCTC TGACGTTTAC TGACATTCAT ATACTTCTTC AGGCCTTCAA GCAACAGCAG 
ATACTTCATG CTCACTACGT CTTAGAGGTG CTATTTGAAG CTAGGAAAGT CCTGAAGCAA 
ATGCCGAATA TCTCTCATGT AAAAACCTTT CCCGCCAAAG AGATAACAAT CTGTGGTGAT 
TTGCATGGGA AACTGGATGA TCTTATGCTT ATTTTCTATA AGAATGGCCT GCCTTCAGAG 
AACAATCCAT ATGTTTTTAA TGGTGACTTT GTAGATCGAG GAAACAATTC CATGGAGATC 
CTAATGATCC TGCTTGTTTG TTTTCTTGTC TACCCCTCTG ACCTGCACCT CAACAGAGGG 
AACCATGAAG ACTTTATGAT GAACCTGAGG TATGGCTTCA CAAAAGAAAT TTTACAAAAA 
TACAAGCTAC ATGGGAGAAA AATCTTACAA GTCTTGGAAG AAGTCTATAC CTGGCTCCCA 
ATCGGTACAA TTATTGACAA CGAAATCCTG GTCATACATG GTGGGATATC AGAATCCACA 
GACTTGAATA CACTCCACCA GTTACAAAGA AATAAAATGA AATCTGTGTT GATGCCGCCA 
GTGTTGATAA ATCAAGAGAC TGGCGTAAAG AAAAATAAAT CAGGTTCAAA TTATGTTGAA 
CCAGACAAGA CTCCTTCTGA AGACTTGACA AAGCAGGAAT GGGAACAAAT AGTTGACATT 
CTGTGGAGTG ACCCCAGAGG CAAAAAAGGC TGTTATCCAA ACACAAGCCG AGGAGGGGGC 
TGCTATTTTG GACCAGATGT TACCTCTAAG GTTCTTAGTA AAAACCAGTT GAAGATGCTC 
ATTAGGTCTC ATGAGTGTAA ACCCGACGGG TATGAAGTCT CTCATGATGG AAAGGTCATT 
ACTGTATTTT CTGCTTCTAA TTATTATGAA GAAGGCAGCA ACCGAGGAGC TTACATCAGA 
CTAAGTTATG GTACAATGCC ACAATTTTTC CAGTACCAAG TAACTAGTAC ATCATGCCTG 
AACCCTCTTC ACCAAAGGAT GAATGCTATG GAATCCAGTG CTTTCAAAAT CCTAAAGGAA 
AAAATGATTT CACGAAAAAC TGATCTCATC AACGCTTTCG AGCTCCGTGA CCACAGTAGG 
TCAGGAAGGA TTTCATTGGC CGAGTGGGCA TTTTCTATGG AGAGCATTTT GGGGCTGAAC 
TTACCATGGA GGTCTCTGAG TTCACACCTG GTAACCATTG ATTCCAGCGG AAGTGTTGAC 
TACATGTCCA GCTTTGACGA TATTCGTATT GAAAAGCCTA TGAAAGATAT GAAGTCCAAT 
CTAACTGAAA CTATGTACAG ATACCGATCT GACCTGAAAA TCATATTTAA TATCATCGAC 
AGTGATCAAT CAGGTCTGAT CTCCATGGAT GAATTTCGGA CCATGTGGAA ACTTTTCAAT 
GCTCATTACA AAGCTCATAT TGATGATTCC CAAATTGATG AGCTTGCCAG CATAGTGGAC 
TTCAACAAAG ATGGCAACAT AGACTTTAAT GAGTTTTTAA AGGCTTTCTA TGTAGTACAC 
AAATATGATA AGCCAGGGAG CAGTCTTGCT TAA